LOCUS       DAEEUE010000001        89763 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.001, whole genome shotgun sequence.
ACCESSION   DAEEUE010000001 DAEEUE010000000
VERSION     DAEEUE010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 89763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89763
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.001"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..258)
                     /locus_tag="KLK84_000001"
     CDS             complement(<1..258)
                     /locus_tag="KLK84_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE2007504.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQI"
     gene            complement(428..811)
                     /gene="rplQ"
                     /locus_tag="KLK84_000002"
     CDS             complement(428..811)
                     /gene="rplQ"
                     /locus_tag="KLK84_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBE2007505.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            complement(852..1841)
                     /gene="rpoA"
                     /locus_tag="KLK84_000003"
     CDS             complement(852..1841)
                     /gene="rpoA"
                     /locus_tag="KLK84_000003"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBE2007506.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            complement(1867..2487)
                     /gene="rpsD"
                     /locus_tag="KLK84_000004"
     CDS             complement(1867..2487)
                     /gene="rpsD"
                     /locus_tag="KLK84_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBE2007507.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            complement(2521..2910)
                     /gene="rpsK"
                     /locus_tag="KLK84_000005"
     CDS             complement(2521..2910)
                     /gene="rpsK"
                     /locus_tag="KLK84_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBE2007508.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            complement(2927..3283)
                     /gene="rpsM"
                     /locus_tag="KLK84_000006"
     CDS             complement(2927..3283)
                     /gene="rpsM"
                     /locus_tag="KLK84_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBE2007509.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            complement(3430..3546)
                     /gene="rpmJ"
                     /locus_tag="KLK84_000007"
     CDS             complement(3430..3546)
                     /gene="rpmJ"
                     /locus_tag="KLK84_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBE2007510.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            complement(3578..4909)
                     /gene="secY"
                     /locus_tag="KLK84_000008"
     CDS             complement(3578..4909)
                     /gene="secY"
                     /locus_tag="KLK84_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBE2007511.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            complement(4917..5351)
                     /gene="rplO"
                     /locus_tag="KLK84_000009"
     CDS             complement(4917..5351)
                     /gene="rplO"
                     /locus_tag="KLK84_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBE2007512.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            complement(5355..5534)
                     /gene="rpmD"
                     /locus_tag="KLK84_000010"
     CDS             complement(5355..5534)
                     /gene="rpmD"
                     /locus_tag="KLK84_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBE2007513.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            complement(5538..6041)
                     /gene="rpsE"
                     /locus_tag="KLK84_000011"
     CDS             complement(5538..6041)
                     /gene="rpsE"
                     /locus_tag="KLK84_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBE2007514.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            complement(6056..6409)
                     /gene="rplR"
                     /locus_tag="KLK84_000012"
     CDS             complement(6056..6409)
                     /gene="rplR"
                     /locus_tag="KLK84_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBE2007515.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            complement(6419..6952)
                     /gene="rplF"
                     /locus_tag="KLK84_000013"
     CDS             complement(6419..6952)
                     /gene="rplF"
                     /locus_tag="KLK84_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBE2007516.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            complement(6965..7357)
                     /gene="rpsH"
                     /locus_tag="KLK84_000014"
     CDS             complement(6965..7357)
                     /gene="rpsH"
                     /locus_tag="KLK84_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBE2007517.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            complement(7391..7696)
                     /gene="rpsN"
                     /locus_tag="KLK84_000015"
     CDS             complement(7391..7696)
                     /gene="rpsN"
                     /locus_tag="KLK84_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBE2007518.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            complement(7711..8250)
                     /gene="rplE"
                     /locus_tag="KLK84_000016"
     CDS             complement(7711..8250)
                     /gene="rplE"
                     /locus_tag="KLK84_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBE2007519.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            complement(8265..8579)
                     /gene="rplX"
                     /locus_tag="KLK84_000017"
     CDS             complement(8265..8579)
                     /gene="rplX"
                     /locus_tag="KLK84_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBE2007520.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            complement(8590..8961)
                     /gene="rplN"
                     /locus_tag="KLK84_000018"
     CDS             complement(8590..8961)
                     /gene="rplN"
                     /locus_tag="KLK84_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBE2007521.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            complement(9126..9380)
                     /gene="rpsQ"
                     /locus_tag="KLK84_000019"
     CDS             complement(9126..9380)
                     /gene="rpsQ"
                     /locus_tag="KLK84_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBE2007522.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            complement(9380..9571)
                     /gene="rpmC"
                     /locus_tag="KLK84_000020"
     CDS             complement(9380..9571)
                     /gene="rpmC"
                     /locus_tag="KLK84_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBE2007523.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            complement(9571..9981)
                     /gene="rplP"
                     /locus_tag="KLK84_000021"
     CDS             complement(9571..9981)
                     /gene="rplP"
                     /locus_tag="KLK84_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBE2007524.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            complement(9994..10695)
                     /gene="rpsC"
                     /locus_tag="KLK84_000022"
     CDS             complement(9994..10695)
                     /gene="rpsC"
                     /locus_tag="KLK84_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBE2007525.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            complement(10713..11045)
                     /gene="rplV"
                     /locus_tag="KLK84_000023"
     CDS             complement(10713..11045)
                     /gene="rplV"
                     /locus_tag="KLK84_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBE2007526.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            complement(11060..11338)
                     /gene="rpsS"
                     /locus_tag="KLK84_000024"
     CDS             complement(11060..11338)
                     /gene="rpsS"
                     /locus_tag="KLK84_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBE2007527.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            complement(11355..12176)
                     /gene="rplB"
                     /locus_tag="KLK84_000025"
     CDS             complement(11355..12176)
                     /gene="rplB"
                     /locus_tag="KLK84_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBE2007528.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            complement(12194..12496)
                     /gene="rplW"
                     /locus_tag="KLK84_000026"
     CDS             complement(12194..12496)
                     /gene="rplW"
                     /locus_tag="KLK84_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBE2007529.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            complement(12493..13098)
                     /gene="rplD"
                     /locus_tag="KLK84_000027"
     CDS             complement(12493..13098)
                     /gene="rplD"
                     /locus_tag="KLK84_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBE2007530.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            complement(13109..13738)
                     /gene="rplC"
                     /locus_tag="KLK84_000028"
     CDS             complement(13109..13738)
                     /gene="rplC"
                     /locus_tag="KLK84_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBE2007531.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            complement(13771..14082)
                     /gene="rpsJ"
                     /locus_tag="KLK84_000029"
     CDS             complement(13771..14082)
                     /gene="rpsJ"
                     /locus_tag="KLK84_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBE2007532.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            14463..14930
                     /locus_tag="KLK84_000030"
     CDS             14463..14930
                     /locus_tag="KLK84_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBE2007533.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            complement(14927..15403)
                     /gene="bfr"
                     /locus_tag="KLK84_000031"
     CDS             complement(14927..15403)
                     /gene="bfr"
                     /locus_tag="KLK84_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HBE2007534.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(15476..15670)
                     /gene="bfd"
                     /locus_tag="KLK84_000032"
     CDS             complement(15476..15670)
                     /gene="bfd"
                     /locus_tag="KLK84_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HBE2007535.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            complement(15853..17037)
                     /gene="tuf"
                     /locus_tag="KLK84_000033"
     CDS             complement(15853..17037)
                     /gene="tuf"
                     /locus_tag="KLK84_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBE2007536.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVG
                     VLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFR
                     TTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKV
                     LG"
     gene            complement(17108..19222)
                     /gene="fusA"
                     /locus_tag="KLK84_000034"
     CDS             complement(17108..19222)
                     /gene="fusA"
                     /locus_tag="KLK84_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBE2007537.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(19319..19789)
                     /gene="rpsG"
                     /locus_tag="KLK84_000035"
     CDS             complement(19319..19789)
                     /gene="rpsG"
                     /locus_tag="KLK84_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBE2007538.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(19886..20260)
                     /gene="rpsL"
                     /locus_tag="KLK84_000036"
     CDS             complement(19886..20260)
                     /gene="rpsL"
                     /locus_tag="KLK84_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBE2007539.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(20386..20673)
                     /gene="tusB"
                     /locus_tag="KLK84_000037"
     CDS             complement(20386..20673)
                     /gene="tusB"
                     /locus_tag="KLK84_000037"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HBE2007540.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTVQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(20681..21040)
                     /gene="tusC"
                     /locus_tag="KLK84_000038"
     CDS             complement(20681..21040)
                     /gene="tusC"
                     /locus_tag="KLK84_000038"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HBE2007541.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDNLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(21040..21426)
                     /gene="tusD"
                     /locus_tag="KLK84_000039"
     CDS             complement(21040..21426)
                     /gene="tusD"
                     /locus_tag="KLK84_000039"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HBE2007542.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(21426..22148)
                     /locus_tag="KLK84_000040"
     CDS             complement(21426..22148)
                     /locus_tag="KLK84_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBE2007543.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(22315..23127)
                     /gene="fkpA"
                     /locus_tag="KLK84_000041"
     CDS             complement(22315..23127)
                     /gene="fkpA"
                     /locus_tag="KLK84_000041"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBE2007544.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            23348..23566
                     /locus_tag="KLK84_000042"
     CDS             23348..23566
                     /locus_tag="KLK84_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HBE2007545.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(23615..24205)
                     /gene="slyD"
                     /locus_tag="KLK84_000043"
     CDS             complement(23615..24205)
                     /gene="slyD"
                     /locus_tag="KLK84_000043"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBE2007546.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(24300..24500)
                     /locus_tag="KLK84_000044"
     CDS             complement(24300..24500)
                     /locus_tag="KLK84_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HBE2007547.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(24510..26247)
                     /gene="kefB"
                     /locus_tag="KLK84_000045"
                     /pseudo
     CDS             complement(24510..26247)
                     /gene="kefB"
                     /locus_tag="KLK84_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(26247..26798)
                     /gene="kefG"
                     /locus_tag="KLK84_000046"
     CDS             complement(26247..26798)
                     /gene="kefG"
                     /locus_tag="KLK84_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HBE2007548.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            26929..28842
                     /locus_tag="KLK84_000047"
     CDS             26929..28842
                     /locus_tag="KLK84_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE2007549.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            28842..29864
                     /locus_tag="KLK84_000048"
     CDS             28842..29864
                     /locus_tag="KLK84_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBE2007550.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            29858..30076
                     /locus_tag="KLK84_000049"
     CDS             29858..30076
                     /locus_tag="KLK84_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HBE2007551.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            30130..30999
                     /locus_tag="KLK84_000050"
     CDS             30130..30999
                     /locus_tag="KLK84_000050"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HBE2007552.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(31054..31458)
                     /locus_tag="KLK84_000051"
     CDS             complement(31054..31458)
                     /locus_tag="KLK84_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HBE2007553.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            31760..32392
                     /gene="crp"
                     /locus_tag="KLK84_000052"
     CDS             31760..32392
                     /gene="crp"
                     /locus_tag="KLK84_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HBE2007554.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            32443..34533
                     /locus_tag="KLK84_000053"
     CDS             32443..34533
                     /locus_tag="KLK84_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBE2007555.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(34600..35820)
                     /gene="argD/dapC"
                     /locus_tag="KLK84_000054"
     CDS             complement(34600..35820)
                     /gene="argD/dapC"
                     /locus_tag="KLK84_000054"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HBE2007556.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(35906..36469)
                     /gene="pabA"
                     /locus_tag="KLK84_000055"
     CDS             complement(35906..36469)
                     /gene="pabA"
                     /locus_tag="KLK84_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HBE2007557.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(36501..37103)
                     /locus_tag="KLK84_000056"
     CDS             complement(36501..37103)
                     /locus_tag="KLK84_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HBE2007558.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(37093..37260)
                     /locus_tag="KLK84_000057"
     CDS             complement(37093..37260)
                     /locus_tag="KLK84_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HBE2007559.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(37365..37946)
                     /gene="ppiA"
                     /locus_tag="KLK84_000058"
                     /gene_synonym="rotA"
     CDS             complement(37365..37946)
                     /gene="ppiA"
                     /locus_tag="KLK84_000058"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HBE2007560.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            38217..39398
                     /gene="tsgA"
                     /locus_tag="KLK84_000059"
     CDS             38217..39398
                     /gene="tsgA"
                     /locus_tag="KLK84_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HBE2007561.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            39660..42203
                     /gene="nirB"
                     /locus_tag="KLK84_000060"
     CDS             39660..42203
                     /gene="nirB"
                     /locus_tag="KLK84_000060"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HBE2007562.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            42200..42526
                     /gene="nirD"
                     /locus_tag="KLK84_000061"
     CDS             42200..42526
                     /gene="nirD"
                     /locus_tag="KLK84_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HBE2007563.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            42652..43458
                     /gene="nirC"
                     /locus_tag="KLK84_000062"
     CDS             42652..43458
                     /gene="nirC"
                     /locus_tag="KLK84_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HBE2007564.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            43477..44850
                     /gene="cobA"
                     /locus_tag="KLK84_000063"
     CDS             43477..44850
                     /gene="cobA"
                     /locus_tag="KLK84_000063"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBE2007565.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            45097..45264
                     /locus_tag="KLK84_000064"
     CDS             45097..45264
                     /locus_tag="KLK84_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HBE2007566.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            45559..46896
                     /gene="frlA"
                     /locus_tag="KLK84_000065"
                     /pseudo
     CDS             45559..46896
                     /gene="frlA"
                     /locus_tag="KLK84_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            46917..47939
                     /gene="frlB"
                     /locus_tag="KLK84_000066"
     CDS             46917..47939
                     /gene="frlB"
                     /locus_tag="KLK84_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HBE2007567.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            47989..48819
                     /gene="frlC"
                     /locus_tag="KLK84_000067"
     CDS             47989..48819
                     /gene="frlC"
                     /locus_tag="KLK84_000067"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HBE2007568.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            48816..49601
                     /gene="frlD"
                     /locus_tag="KLK84_000068"
     CDS             48816..49601
                     /gene="frlD"
                     /locus_tag="KLK84_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HBE2007569.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            49701..50432
                     /gene="phoB"
                     /locus_tag="KLK84_000069"
     CDS             49701..50432
                     /gene="phoB"
                     /locus_tag="KLK84_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HBE2007570.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(50592..51677)
                     /locus_tag="KLK84_000070"
     CDS             complement(50592..51677)
                     /locus_tag="KLK84_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBE2007571.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPGGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(51689..52993)
                     /locus_tag="KLK84_000071"
     CDS             complement(51689..52993)
                     /locus_tag="KLK84_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HBE2007572.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(53005..53358)
                     /locus_tag="KLK84_000072"
     CDS             complement(53005..53358)
                     /locus_tag="KLK84_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HBE2007573.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(53369..54247)
                     /locus_tag="KLK84_000073"
     CDS             complement(53369..54247)
                     /locus_tag="KLK84_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HBE2007574.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(54244..55470)
                     /locus_tag="KLK84_000074"
     CDS             complement(54244..55470)
                     /locus_tag="KLK84_000074"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBE2007575.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(55471..56634)
                     /locus_tag="KLK84_000075"
     CDS             complement(55471..56634)
                     /locus_tag="KLK84_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HBE2007576.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(56718..57080)
                     /locus_tag="KLK84_000076"
     CDS             complement(56718..57080)
                     /locus_tag="KLK84_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBE2007577.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(57097..58002)
                     /locus_tag="KLK84_000077"
     CDS             complement(57097..58002)
                     /locus_tag="KLK84_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2007578.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(58272..59276)
                     /gene="trpS"
                     /locus_tag="KLK84_000078"
     CDS             complement(58272..59276)
                     /gene="trpS"
                     /locus_tag="KLK84_000078"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBE2007579.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCVYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(59269..60027)
                     /gene="gph"
                     /locus_tag="KLK84_000079"
     CDS             complement(59269..60027)
                     /gene="gph"
                     /locus_tag="KLK84_000079"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HBE2007580.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(60020..60697)
                     /gene="rpe"
                     /locus_tag="KLK84_000080"
     CDS             complement(60020..60697)
                     /gene="rpe"
                     /locus_tag="KLK84_000080"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBE2007581.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(60715..61551)
                     /gene="dam"
                     /locus_tag="KLK84_000081"
     CDS             complement(60715..61551)
                     /gene="dam"
                     /locus_tag="KLK84_000081"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBE2007582.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(61658..62944)
                     /gene="damX"
                     /locus_tag="KLK84_000082"
     CDS             complement(61658..62944)
                     /gene="damX"
                     /locus_tag="KLK84_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HBE2007583.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDSAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(63036..64124)
                     /gene="aroB"
                     /locus_tag="KLK84_000083"
     CDS             complement(63036..64124)
                     /gene="aroB"
                     /locus_tag="KLK84_000083"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBE2007584.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(64181..64702)
                     /gene="aroK"
                     /locus_tag="KLK84_000084"
     CDS             complement(64181..64702)
                     /gene="aroK"
                     /locus_tag="KLK84_000084"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HBE2007585.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(65103..66341)
                     /gene="hofQ"
                     /locus_tag="KLK84_000085"
     CDS             complement(65103..66341)
                     /gene="hofQ"
                     /locus_tag="KLK84_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HBE2007586.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(66253..66657)
                     /gene="hofP"
                     /locus_tag="KLK84_000086"
     CDS             complement(66253..66657)
                     /gene="hofP"
                     /locus_tag="KLK84_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HBE2007587.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKSPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(66647..67087)
                     /gene="hofO"
                     /locus_tag="KLK84_000087"
     CDS             complement(66647..67087)
                     /gene="hofO"
                     /locus_tag="KLK84_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HBE2007588.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(67071..67610)
                     /gene="hofN"
                     /locus_tag="KLK84_000088"
     CDS             complement(67071..67610)
                     /gene="hofN"
                     /locus_tag="KLK84_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HBE2007589.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(67610..68389)
                     /gene="hofM"
                     /locus_tag="KLK84_000089"
     CDS             complement(67610..68389)
                     /gene="hofM"
                     /locus_tag="KLK84_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HBE2007590.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            68509..71061
                     /gene="mrcA"
                     /locus_tag="KLK84_000090"
     CDS             68509..71061
                     /gene="mrcA"
                     /locus_tag="KLK84_000090"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HBE2007591.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(71226..71786)
                     /gene="nudE"
                     /locus_tag="KLK84_000091"
     CDS             complement(71226..71786)
                     /gene="nudE"
                     /locus_tag="KLK84_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HBE2007592.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            72106..74241
                     /gene="igaA"
                     /locus_tag="KLK84_000092"
     CDS             72106..74241
                     /gene="igaA"
                     /locus_tag="KLK84_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HBE2007593.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            74306..74974
                     /gene="yrfG"
                     /locus_tag="KLK84_000093"
     CDS             74306..74974
                     /gene="yrfG"
                     /locus_tag="KLK84_000093"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HBE2007594.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            74985..75386
                     /gene="hslR"
                     /locus_tag="KLK84_000094"
     CDS             74985..75386
                     /gene="hslR"
                     /locus_tag="KLK84_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HBE2007595.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            75411..76280
                     /gene="hslO"
                     /locus_tag="KLK84_000095"
     CDS             75411..76280
                     /gene="hslO"
                     /locus_tag="KLK84_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBE2007596.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(76424..78148)
                     /locus_tag="KLK84_000096"
     CDS             complement(76424..78148)
                     /locus_tag="KLK84_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HBE2007597.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTPMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     FAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            78527..80149
                     /gene="pckA"
                     /locus_tag="KLK84_000097"
     CDS             78527..80149
                     /gene="pckA"
                     /locus_tag="KLK84_000097"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBE2007598.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(80225..81577)
                     /gene="envZ"
                     /locus_tag="KLK84_000098"
     CDS             complement(80225..81577)
                     /gene="envZ"
                     /locus_tag="KLK84_000098"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HBE2007599.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(81574..82293)
                     /gene="ompR"
                     /locus_tag="KLK84_000099"
     CDS             complement(81574..82293)
                     /gene="ompR"
                     /locus_tag="KLK84_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HBE2007600.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            82521..82997
                     /gene="greB"
                     /locus_tag="KLK84_000100"
     CDS             82521..82997
                     /gene="greB"
                     /locus_tag="KLK84_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HBE2007601.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            83094..85415
                     /locus_tag="KLK84_000101"
     CDS             83094..85415
                     /locus_tag="KLK84_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBE2007602.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            85853..86080
                     /gene="feoA"
                     /locus_tag="KLK84_000102"
     CDS             85853..86080
                     /gene="feoA"
                     /locus_tag="KLK84_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HBE2007603.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            86097..88418
                     /gene="feoB"
                     /locus_tag="KLK84_000103"
     CDS             86097..88418
                     /gene="feoB"
                     /locus_tag="KLK84_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HBE2007604.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            88418..88654
                     /gene="feoC"
                     /locus_tag="KLK84_000104"
     CDS             88418..88654
                     /gene="feoC"
                     /locus_tag="KLK84_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HBE2007605.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            88857..89744
                     /gene="rpnA"
                     /locus_tag="KLK84_000105"
     CDS             88857..89744
                     /gene="rpnA"
                     /locus_tag="KLK84_000105"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HBE2007606.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASRLAP"
BASE COUNT        21634 a        24236 c        22435 g        21458 t
ORIGIN      
        1 gatttgaggc tctgttaatt ccttctttct gttggcataa aggatggcgt caagttgagc
       61 gaatgaaact gaatcgggca atggccatgc gataccggat gcaagaaatc gctgaaaaag
      121 cgtatgtatt gtggaatgac tgagacccag acgctgagcg atggcccgga tggtcagttt
      181 atcttcaaat cttaaacgca gggcatcagg caaataagaa cggaagcagg gaatatcttt
      241 tgttgtctgg gaattcatcg ttcgtgtcca tcaatataga tgggcgcgat tgttgccaga
      301 caggacaatt ttcacaagac gtcgctgatg gggcgcttac caaaaagagt acagctattg
      361 tagataagtg gggatactac gggtataaaa aaacccgccg gggcgggttt ttttacattg
      421 cttcagatta ctctgcagca gcttctgctt tctctgaacg atcaaccagc tcgatgtaag
      481 ccatcggcgc gttgtcgcct gcacggaagc cacacttcag aatacgagtg taaccaccgg
      541 cacggctcgc gaaacgcggg cccagttcgt taaacagttt tgccacgatc tcgttatcac
      601 gagtacgggc gaatgccaga cgacgattag caacgctatc agtcttggca agagtaatca
      661 gcggctcaac tacgcggcgc agctctttcg ctttaggcag agtcgtcttg atgatttcat
      721 gacgaaccag tgaacctgcc atattgcgga acatagcctg gcgatggctg ctgttgcggt
      781 tcagttgacg accactctta cgatggcgca tgaccttatc cttctcagta aaaccttaac
      841 ctgtgatccg gttactcgtc agcgatgctt gccggtggcc agttttccaa gcgcatgccc
      901 agagacagtc cacgggaagc cagcacgtct ttaatctcag taagagattt tttaccaagg
      961 ttaggcgttt taaggagctc aacctcggta cgctgtacca gatcaccgat atagtggata
     1021 gcttctgctt taaggcagtt agcagagcgg acagtcaatt ccagatcgtc aacagggcgc
     1081 agcaggatcg gatcgaactc tggtttctct tctttcactt caggctgacg tacatcacgt
     1141 aagtcaacga aagcttccag ttgttcagcc agaatggttg ccgcacgacg aatcgcctct
     1201 tcaggatcga ttgtgccgtt ggtttccatt tcgatgacca gcttgtccag gtcggtacgc
     1261 tgttctacac gcgctgcttc aacattgtag gcaatacgct ccacagggct gtagcatgcg
     1321 tcgaccagca gacggccgat tgggcgctca tcttcttccg aatgaattcg ggtagaagcc
     1381 ggcacataac cacgaccgcg ctgaactttg atacgcatgc taatagacgc gttctcatcg
     1441 gtcaggtggc agatcacgtg ctgcggcttg acgatttcga catcaccgtc gtgggtgata
     1501 tcggctgcag tcacagggcc aatgccagat ttattcaagg taagaataac ttcatctttg
     1561 ccctgaactc tcaccgccag ccctttcagg ttgagcagga tttccaggat atcttcctga
     1621 acgccttctt tggtgctgta ctcatgtagt acaccatcaa tctcaacctc ggtcaccgcg
     1681 caacccggca tcgatgagag cagaatacgg cgcagtgcgt tacccagagt atggccaaag
     1741 ccacgctcta aaggctcaag ggtcaccttg gcgtgcgtcg aactcacttg ctcgatatca
     1801 accaggcgcg gttttagaaa ctctgtcaca gaaccctgca ttgtgtcctc tctttggtac
     1861 taagctttac ttggagtaaa gctcgacgat caggtgttcg ttaatgtccg cagacagatc
     1921 agaacgctcc ggcttacgct taaacgtacc ttccatcttg ccagcatcaa cttccagcca
     1981 agttggcttt tcacgctgct cagccagctc cagagcggct ttcacgcgag actgcttctt
     2041 cgctttctca cgaatgctta caacgtcatt cggactaacc tgataagaag cgatgttaac
     2101 aacacgaccg tttaccataa ttgctttatg gctaaccagc tgacgtgctt ctgcacgagt
     2161 ggcaccgaag cccatacggt atacaacgtt gtccagacga ccttccagca gagccaacag
     2221 gttttcaccg gtgttgcctt tcagacgtgc tgcttctttg tagtagttac ggaactgacg
     2281 ctccagcaca ccatagatac ggcgaacttt ttgcttttca cgcaactgca caccatagtc
     2341 agacagacgc ggtttacgcg caccgtgctg gccaggagct tgttcaattt tacacttggt
     2401 atcgatcgcg cgaacgccag acttaaggaa taagtcggtg ccctcacgac ggctcagctt
     2461 gagcttagga cccaaatatc ttgccatttt ctttctccaa caaacctgga aaacgaggcg
     2521 ttatacgcga cgttttttcg gcggacgaca accgttatga gggatcggag tcacatcagt
     2581 aatgttagtg atgcggaaac ctgcggcgtt cagagcacga atagtagatt cgcggcctgg
     2641 acccggacct ttaaccataa cttccagatt cttgatgccg tattctttca cggcgtcagc
     2701 gcaacgctct gctgcaacct gagctgcaaa cggagtggat ttgcgagaac cacggaaacc
     2761 ggaaccaccg gctgttgccc aacccaacgc gttaccctga cgatcagtga tagtcacgat
     2821 ggtgttgttg aaagaagcat ggatatgagc cacgccgtca gagacttgtt ttcttacacg
     2881 tttacgtgca cgaattggtg cctttgccat tattcaatca ccccgattat ttcttgatcg
     2941 gtttgcgcgg acccttacgg gtacgtgcgt tggtcttggt acgctgaccg cgaaccggga
     3001 gaccacgacg atgacgcaaa ccgcgatagc aaccaagatc catcaggcgc ttgatgctca
     3061 tgctgatttc acggcgcaga tcaccttcaa cgacaaattt ggcaacttcg tcacgcagcg
     3121 tgtcgatttg tccttcagac agctcactga tcttaacatc ttcagcgata cccgctgcag
     3181 ccaggatggc tttagaacgg gtcttgccga cgccataaat cgaagttaat gcgattacgg
     3241 catgcttatg atcaggaatg ttaatgcctg ctatacgggc cactatgcac tcctactatt
     3301 taatatgtac gttccatgct gaaaagcccg ttttcaggat actcaaatgg aaacgtacag
     3361 acatacaaaa gattggctgg ctaatctagc cagctcaacc caactttgca agaaaaatat
     3421 gcgaaaaaat cagccttggc gctgtttatg cttcggctcg gcactgcaaa tcacacggat
     3481 gacaccatca cgcttaacga ttttgcagtt acggcataat ttcttgacgg aagcacgaac
     3541 tttcattttt actctccgta acttctcggg cgaccaatta tcggccgtag cctttcaggt
     3601 tcgccttctt caatgcagac tcatactgac tggacatcat cagagtttgc acttgagcca
     3661 taaagtccat aatcacgaca acaacgataa gcagtgaggt cccaccgaag tagaacggta
     3721 ctttcattgc atcacgcatg aactccggga tcaggcagat aaaggtaata tacagcgcac
     3781 caaccaaggt caggcgggtc attactttat cgatatactt cgccgtttgc tctcccggac
     3841 gaattcctgg tacaaatgca ccggacttct tcaggttatc tgctgtttca cgcgggttga
     3901 aaaccaacgc cgtgtagaag aaacagaaga agatgattgc agacgcatag agtaacacat
     3961 aaagcggttg cccaggctgc aaatacagcg aaattgttgt cagccagttc caaccagtac
     4021 cgcccccgaa ccatgacgcg atggtcgccg ggaacagaat aatactggaa gcgaagattg
     4081 ccgggattac ccccgccata ttcactttca gcggtaaatg tgtgctctgt gcagcataga
     4141 cacgacgacc ttgctgacgt ttcgcgtagt ttaccacaat gcggcgttga ccacgctcaa
     4201 caaacacaac aaagaacgtc actgcaaata ctaatactgc aaccaacagc aacacgagga
     4261 agtgcaggtc gccttgacgc gcttgctcga tagtatgggc aatggctggc gggagtcccg
     4321 cgacaatacc ggcgaagata atgattgaaa taccgttgcc gatacctcgt tcagtgatct
     4381 gttcgcccaa ccacatcagg aacatcgttc ctgtgaccag acttacaaca gcggtgaagt
     4441 agaatgcaaa gcccgggtta atcaccaggc cttgcatacc aggcatattc ggcagaccgg
     4501 tagcaatacc gatcgactgg aatattgcca gcaccagagt accgtaacgg gtgtactggc
     4561 tgatcttacg acgaccagac tccccttctt tcttaatttc tgccaacgtt gggtgaacca
     4621 ccgtcagcag ctggataatg atcgacgccg aaatatacgg catgatcccc agagcaaaga
     4681 tagaagcacg gctgagagca ccaccagaga acatgttaaa catctcaatg atggtgcctc
     4741 gctgttgctc aagcagtttg gcaagtacag cggcatcaat accagggatc ggaataaaag
     4801 agccaatacg gaacacaatc agcgcaccga taacaaacag cagtctgcgt ttcagctcgc
     4861 ctaagccacc tttggcactt tgaaaatcta atcccggttg tttagccatc tgctacttat
     4921 tcctcgattt taccgccagc agcttcgata gcagcacgag cgcctttagt aacacgcagg
     4981 ccacgaacag ttaccggagt cgttacttcg ccagccagga tcactttcgc gaactcgatc
     5041 tggataccga taatgttagc cgctttcagc gtgttcaggt ctacaacacc gccttctact
     5101 ttagccaggt cagatagacg aacttcggct gtaatcgctg ctttacgaga agtgaagccg
     5161 aatttcggca gacgacggta cagaggcatc tgaccaccct cgaaaccgcg acgtacgcca
     5221 ccgccagaac gagacttctg acctttgtga ccacgaccac cggttttacc gaggccagaa
     5281 ccgataccac gacccaggcg tttacccgcc tttttggagc cttcggccgg agacagagta
     5341 tttaaacgca tctcttactc ctcaacttta accatgaagg aaaccgcgtt gatcataccg
     5401 cgaatagcag gagtatcctc gcgctctacg gtgtgaccaa tacgacgcag acccaagcca
     5461 agcagcgttg ccttgtgttt cggcagacga ccgattgcac tgcgggtttg agtaatttta
     5521 atagtctttg ccatggttta tttccccaga atttcttcaa cggatttacc acgcttggca
     5581 gcgaccattt ctggagaatt catattttcc aggccatcaa tagttgcacg aaccacgttg
     5641 atcgggttgg tggaaccata tgctttagcc agaacgttat gaaccccagc gacttccaga
     5701 acggcgcgca ttgcaccacc ggcgatgata ccggtacctt cggaagccgg ctgcatgaat
     5761 acgcgagaac ccgtgtgaac acctttaaca gggtgttgta gagtgccgtt attcagcgcg
     5821 acgttaatca tattgcgacg ggctttttcc atcgctttct ggatcgctgc tggaacttca
     5881 cgcgctttac cgtaaccaaa accaacgcga ccgttaccat cgccaactac agtcagagct
     5941 gtgaaggaga aaatacgacc acctttaacg gttttagata cgcggtttac cgcgatcagc
     6001 ttttcctgca gttcgccagc ttgtttttcg atgtgagcca tcttacacct ctaccttaga
     6061 actgaaggcc agcttcacgg gcagcatctg ccagtgcctg gacacgacca tgatattgga
     6121 acccggaacg gtcaaaggat acatctttga tgcctttttc cagagcgcgt tcagcgacag
     6181 ctttacccac agctgcagcc gcgtctttgt taccggtgta cttcagttgt tcagcgatag
     6241 ctttttctac agtagaagca gctaccagaa cttcagaacc gttcggtgca attacctgtg
     6301 cgtaaatgtg acgcggggta cgatgtacca ccaggcgagt tgcgcccagc tcctggagct
     6361 tgcggcgtgc gcgggtcgca cgacggatac gagcagattt cttatccata gtgttacctt
     6421 acttcttctt agcctctttg gtacgcacga cttcgtcggc gtaacgaaca cccttgcctt
     6481 tgtaaggctc aggacgacgg taggcgcgca gatccgctgc aacctggccg atcacctgct
     6541 tatcagcgcc tttcagcacg atttcagtct gagtcggaca ttcagcagtg atacccgcag
     6601 gcagctgatg gtcaacagga tgagagaaac ccagagacag gttaatcaca ttgcctttaa
     6661 ccgctgcacg gtaacctaca ccaaccagct gcagcttctt agtgaagcct tcggtaacac
     6721 cgataaccat tgagttcagc agggcacgcg cggtaccagc ctgtgcccaa ccgtctgcgt
     6781 aaccatcacg cggaccgaag gtcagggtat tatctgcatg tttaacttca acagcatcgt
     6841 tgagagtacg agtcagctcg ccgtttttac ctttgatcgt aataacctga ccgttgattt
     6901 ttacgtcaac gccggcagga acaacgaccg gtgctttagc aacacgagac attttttcct
     6961 ccgattaggc tacgtagcag ataatttcgc caccaagacc agcctggcgc gctgcacgat
     7021 cagtcataac acctttagag gtagaaacaa ctgcgatacc cagacccgcc ataactttcg
     7081 gcagctcatc tttacgttta tagatgcgca gacctgggcg gctgacacgc tgaatgcttt
     7141 ctacaacagc tttgccctgg aaatacttca gagtaagttc cagttcaggc ttggtgtcgc
     7201 cttcaacttt aaaatcttca ataaaacctt cttccttcag cacgttggcg attgccactt
     7261 tcagcttgga ggaaggcatg gtgaccgcag ctttgttcgc ggcctgaccg ttacggatac
     7321 gggtcagcat atccgcgatc ggatcttgca tgctcatctg tctttactcc cgtgattcaa
     7381 ttggtgacaa ttaccagcta gcctttttca gacccgggat ttcaccgcgc atagcggctt
     7441 cacggacctt aatacggctc aacccgaact tccgcaggaa accatgcgga cgacctgttt
     7501 gacggcagcg gttacgctga cgagacgggc tggaatcacg cggcagagtc tgcagcttga
     7561 gaacagcgtt ccaacgatct tcgtcggaag cgttcacatc agagatgatc gctttcagtt
     7621 cagcgcgttt cgcgaagtat ttatcagcta aagctacgcg ttttacttcg cgtgctttca
     7681 ttgattgctt agccatttag taaccctacc ttacttgcgg aacgggaagt caaaggcagc
     7741 cagcagagcg cggccttctt cgtcagattt cgcagtagtg gtaatggtaa tatccaaacc
     7801 acgaacgcgg tcgactttat cgtagtcgat ttctgggaag atgatctgct cacggacacc
     7861 catgctgtag ttaccacgac cgtcgaaaga cttagcggac aggccacgga agtcacggat
     7921 acgaggtaca gcaatagtga tcaggcgctc aaagaactcc cacatgcgtt cgccacgcag
     7981 agttacttta cagccgatcg gatagccctg acggattttg aagcctgcaa cagatttgcg
     8041 tgctttggtg atcagcggtt tttgaccgga gattgctgcc aggtctgctg ctgcgttatc
     8101 cagcagtttt ttgtcagcga tcgcttcacc aacacccatg ttcagggtga tcttctcgac
     8161 ccgagggact tgcatgacag aattgtagtt aaactcagtc atgagttttt taactacttc
     8221 gtctttgtag taatcatgca gtttcgccat cgtactactc caaattactt gatagtttcg
     8281 ctgttagact tgaagaaacg gacttttttg ccgtcttcga atctaaagcc tacacggtca
     8341 gccttgccgg ttgccgcatt gaagattgct acgttggaaa cctgaatagc ggcttctttt
     8401 tcaacgatgc cacccggttg gttcagggcc ggaaccggct tctgatgttt cttaaccagg
     8461 ttgatacctt caacaatgac cttgccggaa gacaggacat tcttaacttt accgcgttta
     8521 cctttatctt taccggttaa cacgataact tcgtcatcac gacggatttt cgctgccatg
     8581 attcgctcct tagagtactt ctggtgccag agagataatt ttcatgaact tctcactacg
     8641 aagctcacga gttaccggcc caaaaatacg cgtaccgata ggctgctcgc tgttgttgtt
     8701 cagaagaaca caagcattac catcgaagcg aatgacagaa ccgtccgggc gacgaacacc
     8761 cttcttggtg cgcaccacta ccgccttcag cacatcacct tttttgacct taccacgcgg
     8821 aattgcttct ttgatggtga tcttgatgat gtcgcctacg cctgcgtagc gacggtgcga
     8881 gccacccaga accttgatac acattacgcg acgtgcaccg gagttgtcgg cgacgttcag
     8941 catagtctgt tcttggatca ttttagtgct ccgctaatgt caactactac tgagacccga
     9001 aaatcaggtc gttaaaaatc cccatatcga gggcgcggca ttataacacc gcttcaagga
     9061 tatgggtaga aaaaataaac ggctcatttc tgagccgttt attcgtatcg agagagtgta
     9121 ctgtattaca gaaccgcttt ctctacaacg cgaaccagcg tccaggattt agtcttggac
     9181 agcggacggc attcgcggat ttcaaccacg tcaccgatac cgcattcgtt gttctcgtca
     9241 tgtacgtgca gtttggtcgt acgcttgatg aatttaccgt agatcgggtg tttcacaaaa
     9301 cgttcgatag caacaacaat ggatttctcc attttgtcgc taacaacgcg accttgcaga
     9361 gtacggattt tatcggtcat tacgcacccg ccttctcgtt cagtaaagtc ttaacgcgtg
     9421 cgacatcgcg acgcacttgc ttcaacaggt gagactgttg cagctggcca cttgcagcct
     9481 gcatacgcag gttgaactgc tcacgcagca ggttcagcag ctcggtgttc agctcttcaa
     9541 tgctcttctc acgcagctct tttgctttca ttacatcacc gtcttagtta caaaggtggt
     9601 tttaatcggc agtttcgctg ctgccagctt gaatgcttca cgggccagct cttccggaac
     9661 accgtccatt tcatacagga ctttacccgg ctgaatcaag gcaacccaat actccacgtt
     9721 acctttacct ttacccatac gcactgccag cggcttttca gtgatcggtt tgtccgggaa
     9781 cacacggatc cagatcttac cttgacgctt aactgcacgg gtcatagcac gacgtgctgc
     9841 ttcgatctga cgggcagtca gacgaccacg gccaacagct ttcagaccga agctgccgaa
     9901 gctaacatcc gtaccctgcg ccagaccgcg gttacggcct ttgtgcattt tacggaattt
     9961 tgtacgcttt ggttgtaaca tcagcgacgc tccttattta cggcctttac gctgctgctt
    10021 tttaggctga gcagccggtt tttccggttg ttcaacagca gccataccac ccaggatctc
    10081 gcctttgaag atccacactt taacgccgat tacaccgtaa gtggtgtgcg cttcagaggt
    10141 gttgtagtcg atgtcagcac gcagagtgtg cagcggtacg cgaccttcgc ggtaccattc
    10201 ggtacgtgcg atttccgcgc cgcccagacg gccgctaact tcaactttaa tacctttagc
    10261 gcccagacgc attgcgttct gtacagcacg cttcatagca cgacggaaca taacgcgacg
    10321 ttccagctga gaagtgatgc tgtcagcaac cagttttgcg tccagttcag gcttacgaac
    10381 ttcggcgatg ttgatctgtg caggaacgcc agcgatgtcc gctacgacct tacgcagttt
    10441 ttctacgtct tcaccttttt taccgataac gatacccggg cgagcagtgt gaatggttac
    10501 acggatgctc ttagccggac gctcgataac gatacgagat acggacgctt tagccagttc
    10561 cttagtcagg tactgacgta ctttaaaatc gctgtccagg ttgtcagcga attctttggt
    10621 gttcgcaaac caagtagagt tccatggttt tacaataccc aagcgaatac cattaggatg
    10681 tactttctga cccattgcta gtctccagag tctcagcgat cggacacaac cacagtgatg
    10741 tggctggtgc gcttcaggat gcgatctgca cgaccttttg cacgcggcat aatgcgcttc
    10801 atgctcgggc cttcgtctac gaaaattttc gtaactttca gatcgtcaat gtcagcgcca
    10861 tcgttgtgtt cagcgttagc aatggcagat tccagaactt tcttgacctg tacagccgct
    10921 ttcttgttgg tgtaggtcaa aatatccaga gcctgcgaca ctttcttacc gcgaatcagg
    10981 tcagcaacaa ggcgaacctt ctgagcagaa gaacgagcat ggcgatgttt agcaatagtt
    11041 tccatctctt cctcctacct tatttcttct tcgctttttt atcagcagcg tggccgcgat
    11101 aagtacgagt cggtgcgaat tcacccagtt tgtgaccgac catttcgtcg gttacaaata
    11161 ccggaacgtg ctgacgacca ttatggacag cgatggtcaa accgatcatg ttaggaaaga
    11221 tcgttgaacg acgggaccaa gtgcgcaggg gcttcttgtc tccgctttcc accgctttct
    11281 ctaccttctt cagcaagtgc aggtcaataa aaggaccttt cttgagagaa cgtggcatgg
    11341 cttatcctct aaaattattt gctacggcga cgtacgatga atttatcagt acgcttgttg
    11401 ctgcgggtct tcttaccttt ggtctgaacg ccccacggag ttaccgggtg cttaccaaag
    11461 ttacgacctt caccaccacc atgtgggtgg tctaccgggt tcatcgcggt accgcgaacg
    11521 gtcggacgaa caccacgcca gcgtgcagca cctgctttac ccagaacgcg cagcatatgc
    11581 tcagcattgc caacttcgcc cagagttgca cggcagtctg cttctacttt acgcatttca
    11641 ccagaacgca gacgcagggt gacataagca ccatcacgag caacgatctg aacgtaagta
    11701 ccagcggaac gtgccagctg accgccttta cctggtttca tttctacgtt atgaacagta
    11761 gaaccaaccg ggatgttgcg catcggcagg gtgttacctg gtttgattgc agcatcaacg
    11821 ccagactgaa tctggtcgcc agctttcagg cctttagggg ccaggatgta acggcgttca
    11881 ccgtctttgt acagaaccag cgcgatgttc gcggaacggt tcggatcgta ctcaagacgt
    11941 tcaacaactg ccgggatacc gtctttgttg cgtttgaagt caacaatacg gtaagcctgc
    12001 ttgtggccac caccgatatg acgagtggtg atacggccat tgttgttacg accaccggat
    12061 ttgctgtttt tttccagcaa cggagcaaaa ggtttgccct tgtgcagctc agggttaacc
    12121 actttaacta cgtggcgacg acccggagat gtcggtttac atttaacaac tgccattgta
    12181 ttactcctcc gacttactca gcgccgccaa cgaagtccag attctggcct tctttcaggg
    12241 tgacgtaagc ttttttccag tcgctacgac gaccgatacg ctgtccgtga cgtttaactt
    12301 tccctttaac taccagggtg ttaacgactt cgacttcgac ttcaaacagt ttctgcacag
    12361 cagctttgat ttctgctttg gtcgcgtctt tcgcaacttt gagtacgatg gtgttggatt
    12421 tttccatcgc agtagacgct ttttcagaaa cgtgcggtgc acgcagcacc ttcagcagac
    12481 gttcttcacg aatcatgcca gcatctcctc aacttgctta acagcatcag cagtcattac
    12541 gactttgtcg aaggcgatca ggctaaccgg gtcgatacca gttgcatcgc gtacgtcaac
    12601 cttctgcagg ttgcgcgcag ccaggaacag gttttcgtcc agctcaccgg tgatgatcag
    12661 cacatcttcc agagccatgt ctttcagttt ctgtgccagc agcttagttt tcggcgcttc
    12721 tacagagaac ttctcgacaa cgatcagacg atcctgacgt accagttcgg acaggatgct
    12781 tttcagcgcg ccgcggtaca tcttcttgtt aactttttga ctgtggtcct gcggacgagc
    12841 agcaaaggtc acgccaccag aacgccagat cgggctcttg atagaaccag aacgcgcacg
    12901 gccggtgcct ttctggcgcc acggtttttt accggaacca gttacttcag cacgagtctt
    12961 ctgagcacga gtaccctgac gagcaccagc tgcataagca acaacaacct ggtgaaccag
    13021 cgcttcgttg aaatcacgac cgaaggtagt ttcggaaaca gtcagcgcgc tctgcgcgtc
    13081 tttcaatact aattccattg ctatctcctt acgccttcac agctggttta acgatcaggt
    13141 cgctaccggt tgcacccggg acagcacctt taaccagcag caggttgcgc tcagcgtcaa
    13201 cgcgtactac gtcaaggctc tgaacggtta cacgttcgtt acccatctga cctgccattt
    13261 tcttgccttt gaacactttg cccggagtct ggttctgacc gatagaaccc ggaacgcggt
    13321 gagacaagga gttaccgtga gtagcgtcct gggtacggaa gttccagcgc ttaacggtac
    13381 ctgcgaaacc tttaccttta gaggtgccag ttacgtcaac ttttttaacg tcagcaaaca
    13441 gttcaacgct aatgctctga cctacagtga actcttcgcc ttcagccagg cggaattccc
    13501 acagaccacg gccagcttct acgccagctt tagcgaagtg gccagcttca ggcttggtca
    13561 cacggttagc ttttttagca ccggtggtca cctgaatagc acggtagcca tcgttagcca
    13621 ggtctttaac ctgagtaacg cggtttgctt caacttcgat tacggttact gggatagaaa
    13681 cgccgtcttc tgtgaagata cgggtcatac ccactttttt accgactaaa ccaatcattg
    13741 tttcaacctc tcaatcgctc aatgacctga ttaacccagg ctgatctgca cgtctacacc
    13801 ggcagccaga tccagacgca tcagagcatc aacggttttc tcggttggct caacgatgtc
    13861 aaccagacgc aagtgagtac ggatttcgta ctgatcgcgc gcgtctttgt tgacgtgcgg
    13921 ggagatcaga acagtgaagc gctctttgcg tgtcggcagc gggatcggac cacggacctg
    13981 cgcaccagtg cgcttggcag tctcgacgat ttccgcggtt gcttgatcga tcagacgatg
    14041 atcaaacgct ttcaggcgga tacggattct ttggttctgc atgagaccag agctccaatt
    14101 attttataaa cgaaaatgat tactcctcag acccattacg attgatggga gagtgtaacc
    14161 gttcttacgt agctccccga ttgggagcat tgttaggtag ccaaattcgg ctaactgagg
    14221 ttcagattga acctgctgtc aactacgaca agcccgcgca ttatacgcaa atctgagcct
    14281 gacgcaagca tcgggtagaa attaatcgca acgcgcagat tgcgagctgc attccacgaa
    14341 atttgtaagg ttgtgaagat agcgtttttc accggaatca ggttaacaaa tcagcgctct
    14401 ctctctgttg ctgagcatcc ggagttattc gatggtgata ctccaattac agaggatgtt
    14461 ttatggacac tacccttccc tttctaattt tgtacgcttg tttatcagcc ctactttttt
    14521 tctgggatgc aaagcatggc ttgttacctg acagatttac gtgcccgtta ctctggtcag
    14581 gtcttttgtt ctatcaagtt tgtcatcctg atggtttagc cgatgcgtta tggggggcaa
    14641 ttgttggtta cggcacattc gctgtcattt actgggggta tcgcatactg cgacataaag
    14701 aaggattagg ctatggtgat gtgaagtttc tcgccgccct tggcgcctgg cattcatggg
    14761 ccttcttacc acgattggtt ttccttgctg cctcattcgc ttgcggagcc gtagttattg
    14821 gtctgctcat gagaggaaaa gaatcattaa aaaacccgct gccttttggg ccatttctgg
    14881 cggctgcggg tttcgttata ggctgggata gtttgctgat aggccgttaa tctttaactt
    14941 taatttgtga ttgcaggtaa ttttgtaggc cgattttacc gatgagatcc agttcagttt
    15001 cgagccagtc gatatgacct tcttcatcag caaggatctc gatcatcata tcgcggctaa
    15061 catagtcatg aacgctgtcg gcatatgcga tagcctcacg cagatccttc gcgccttcca
    15121 gttctaatcg caggtcagat tgcagcatct cctcgacatc ttcaccaatc cccagtttgc
    15181 ctaaatcctg taagttgggg attccttcta aaaacaaaat acgctcgata tatttatcag
    15241 cgtgtttcat ctcatcgatg gattcatggt attcgacgtc attgagacgc atcaggcccc
    15301 agtttttaaa catccgggca tggagaaaat actgattgat tgcgacaagc tcatttccca
    15361 atagtttatt gagataattt atgattttaa catcaccttt cattttatag tccctccgct
    15421 tccactatta gagcgtagat ggggctagca ggatgtcaaa aaataagcgt gggcttcagg
    15481 caatctcttt aaattctggc atctgcatca actcgtcctg catcacttcg cgcgcggcgc
    15541 gaatgcactt accgcattga tttcccacag gaataaactt acgcaattgt tggaaagatt
    15601 gcggatgaaa ctgacggact gcctggcgaa tttttttgtc acttacacca ttacacaaac
    15661 aaacgtacat gattgctccc gttcaaattc tgcacaaagt gtaaatgaga atggttatga
    15721 ttacaatagt acagttttat ttgcgccata gttcagcgag caaaattacg actaaatatt
    15781 tcgagctcaa attacgggca gcaaaaaggg cgccgaagcg cccttttcaa ttcaaaacta
    15841 attaacgtgt aattagccca gaactttagc aacaacgccc gcgccaacgg tacggccgcc
    15901 ttcacggatt gcgaaacgca gaccgtcgtc catcgcgatc gggtggatca gggtaacaac
    15961 cattttgatg ttgtcgcccg gcattaccat ctctacgcct tccggcagtt cgatggtacc
    16021 agtcacgtca gtagtacgga agtagaactg cggacggtag cctttgaaga acggagtatg
    16081 acggccgcct tcatctttgg acagaatgta cacttcagat tcgaacttgg tgtgcggctt
    16141 gatggtgccc ggcttagcca gtacctgacc acgttcgatt tcttcacgtt tgataccacg
    16201 cagcagaaca cctacgttct caccagcacg gccttcgtcc agcagtttgc ggaacatttc
    16261 aacgccagta caggtagact tctgagtctc tttgatacca acgatttcaa cttcttcacc
    16321 aactttgatg ataccgcgtt ctacacgacc ggtaacaacg gtaccacgac cggagatgga
    16381 gaatacgtct tcgatcggca gcaggaacgg cttgtcaatc gcacgctctg gttccggaat
    16441 ataagaatcc aggaagccag ccagttccag gattttcgct tcccactctg cgtcgccttc
    16501 cagcgctttc agagcagaac cacgaacgat cggagtgtcg tcgcccggga agtcgtactg
    16561 agacagaagt tcacgaactt ccatttcaac cagttccagc agctcttcgt catcaaccat
    16621 gtcgcatttg ttcaggaaca cgatgatgta cggaacgcct acctgacgac ccagcaggat
    16681 gtgctcacga gtctgcggca tcgggccgtc agtcgcagca actaccagga tcgcgccgtc
    16741 catctgagca gcaccggtga tcatgttttt aacatagtcg gcgtgccccg ggcagtctac
    16801 gtgtgcgtag tgacgggtcg gggtgtcata ttcaacgtga gaagtgttga tggtgatacc
    16861 acgagctttt tcttccggcg cgttatcgat ctggtcgaat gcacgagcag caccgccgta
    16921 ggttttagcc agtacggtgg tgattgcagc ggtcagagta gttttaccgt ggtcaacgtg
    16981 gccgatagta ccaacgttaa cgtgcggttt tgtacgttca aatttttctt tagacacggc
    17041 tatattcctt actatagtgc tctccccttc aggagagagc acgggacttt ggtattaacc
    17101 cttaggctta tttaccacgg gcttcaatta cggcctgagc aacgttactc ggcgcttcat
    17161 catacttcag gaattccata gtgtatgatg cacgaccttt ggtcagagaa cgcagctgag
    17221 ttgcgtatcc gaacatttca gacagcggta cttcagcgtg gatcttaacg ccagtaactt
    17281 cagattcctg acctttgagc ataccacgac gacggctcaa gtcaccgata acgtcaccgg
    17341 tgttctcttc cggagtttct acttcaacct tcatgatcgg ctcaagcaga actggtttcg
    17401 ctttcttaaa gccttcttta aaggcgatag aagcagccag tttaaacgcc agttcagagg
    17461 agtcaacgtc atggtaagaa ccgaagtgca gacgaatacc catgtctact accgggtagc
    17521 ctgccagcgg acctgctttc agctgttcct ggataccttt atcaacggcc gggatgtatt
    17581 cgccagggat tacaccacct ttaatgtcgt tgatgaactc gtagcctttc gggtttgaac
    17641 ccggctccag cgggtacatg tcgataacaa catgaccata ctgaccacga ccaccagact
    17701 gtttcgcgtg tttaccttca acatcggtaa ctttctggcg gatagtttca cggtaagcaa
    17761 cctgcggttt acctacgttc gcttcaacgt tgaattcacg cttcatacgg tcaacgatga
    17821 tgtcgaggtg cagttcgccc atacccgcga tgatggtctg gttagattct tcgtcagtcc
    17881 atacacggaa agacgggtct tctttagcca gacggcccag agccagaccc attttttcct
    17941 ggtcagcttt ggttttcggt tcaactgcga tggagattac cggctcaggg aattccatac
    18001 gttccagaat gatcggcgca tccgggtcac acagggtgtc accagtggtt acgtctttca
    18061 gaccgatagc agcagcgatg tcgcccgcgc gaacttcttt gatctcttca cgtttgttag
    18121 cgtgcatctg aacgatacga ccgaaacgct cacgtgcagc tttcacggag ttcagtacgg
    18181 tatcaccaga gttaaccaca ccggagtaaa cacggaagaa ggtcaggtta ccaacaaacg
    18241 ggtcggtagc gattttgaac gccagtgcag agaacggctc gtcatcactt gcgtgacgtt
    18301 cagccggagt gtctttaccg tcgtccagga taccgttgat cgcaggtacg tcaaccgggg
    18361 atggcaggta atcaattacc gcatccagca tcgcctgaac acctttgttc ttgaacgcag
    18421 aaccacaggt taccaggatg atttcgttgt tcagaacgcg ctgacgcaga gcacctttga
    18481 tttctgcttc agtcagttct tcaccaccca ggtatttttc catcagctct tcagaagctt
    18541 cagctgcgga ttcgatcagg ttctggtgcc attcgttagc cagttcaacc atgtctgccg
    18601 ggatatcttc gtattcgaag gttacgccct ggtcagcgtc gttccagttg atagctttca
    18661 ttttcaccag gtcaacaaca ccggtgaaat gttcttcagc accaatcgcc agctgcagcg
    18721 gaaccgggtt cgcgcccaga cgggttttga tctggttaac aactttcagg aagttcgcac
    18781 ccacgcggtc cattttgtta acgaacgcaa tgcgcggaac tttatatttg tttgcctgac
    18841 gccatacggt ttcagactgc ggctgaacac caccaactgc gcagtaaacc attaccgcac
    18901 catcgagaac acgcatggaa cgttctactt cgattgtgaa gtcaacgtgc cccggggtgt
    18961 cgatgatgtt gatgcgatgc ggctcatact gcttagccat accagaccag aatgcagtag
    19021 tcgcagcgga agtgatggta ataccacgtt cctgctcctg ctccatccag tccatggttg
    19081 cagcgccgtc atgaacttca ccgattttat ggtttacacc ggtgtagaac agaatacgtt
    19141 cggtagtagt ggttttaccg gcgtcgatgt gcgcactgat accgatgtta cggtagcgtg
    19201 cgatgggtgt tgtacgagcc atttgtttcc tcgtttatct tttaggcgtt caatttaagt
    19261 agcccaaagc gggctgctta ctggaagcgc ccgcctggtg actaaaactc cgaagggatt
    19321 accaacggta gtgtgcgaac gccttgttgg cttcggccat acggtgaacg tcttcacgtt
    19381 tcttaactgc agtacctttg ttttctgcag catcagaaag ttcgttcgcc aggcgcagag
    19441 ccatggattt atcaccgcgt ttacgagcag cttcaacgat ccaacgcatt gccagagcat
    19501 tacgacgaac cggacggact tcaactggta cctgataagt agaaccacca acgcggcgag
    19561 acttaacttc tacagtcggg cgcacgtttt cgagagctac ttcgaatgct tccagttcag
    19621 atttaccaga gcgctgagcc agggtctcca gcgcgctgta tacgatagat tcagcagtag
    19681 attttttacc atctaccatc aggatattta caaatttagc cagcagttct gatccgaact
    19741 tcggatccgg cagaatttta cgctgaccaa tgacgcgacg acgtggcatg gaaatactcc
    19801 gttgttaatt caggattgtc caaaactcta cgagtttagt ttgacattta agttaaaacg
    19861 tttggcctta cttaacggag aaccattaag ccttaggacg cttcacgcca tacttggaac
    19921 gagcctgctt acggtcttta acgccggagc agtcaagcgc accacgtacg gtgtggtaac
    19981 gaacacccgg gaggtcttta acacgaccgc cacggatcag gatcacggag tgctcctgca
    20041 ggttgtgacc ttcaccaccg atgtaggaag tcacttcgaa accgttagtc agacgaacac
    20101 ggcatacttt acgcagcgcg gagttcggtt ttttaggagt ggtagtatat acacgagtac
    20161 atacgccacg tttttgcggg catgcttcca gcgcaggcac gttgcttttc gcaactttgc
    20221 gagcacgtgg tttgcgtacc agctggttaa ctgttgccat taaatagctc ctggttttag
    20281 cttttgcttc gtaaacacgt aataaaacgt cctcacacaa tatgaggacg ccgaatttta
    20341 gggcgatgcc gaaaaggtgt caagaaatat acaacgatcc cgccatcacc aggccatctg
    20401 gctggagtgc ttaaccgtaa gtctgacgaa atcagtatag tcaatgggaa tgatgtcgtt
    20461 caaaatttga acagtcaaac cgcgggcaat aaggtcttcg ttcagggcat agaccttaat
    20521 gggggcatta cgcagacttt caaggtagcg gttaccgtca actgcggcag ttacgccatc
    20581 ttgcaataat agcagttcgt ctccttcact gagcagacgc agcagcgcag caaaatccgt
    20641 cagccagggt gaacgatgta acgtgtgcag cataaacgcc tcaaaacctc aaaataacat
    20701 cgtagttggc gagttcgcgg cgtaaggcat ctgcttcgag cggcgtggct tcgacaacaa
    20761 agggtgtctg cggatctaac ccgcgttcgc gcagtgaagc cgcacaaacc cagcactgtt
    20821 caatgtcgta cagactcaac aatttaaaag tggcaatgta atcacgcgcc agcactgcat
    20881 cgggcttttg tcctgacagc agctgaaaaa cgccatcagc aataaagaag acggccagat
    20941 tgtcagttaa tgcggaagtt gccagtaaag catctaaacc ttcccgacca gcagctgtac
    21001 catgaggtgc agtagaaaaa acaaacgcaa ttcgtttcat cagaactgta ccaccctgtc
    21061 acaggtcagc gaggcttccg ccagcgcgcc aagtccgctt aaggtaaatc ccggctgaag
    21121 gtttgacgaa gccagcccca gccttcctgc ttcagtttca tcgacgatac cgcggcgtaa
    21181 cgctgccgct acgcagatat tcagcgccac accatgttgc gcattcaact gttgccagcc
    21241 gcgcacaagg tcaaattcgt cgcttgccgg tgaggtcagt tggttagcgt tatagacccc
    21301 ttcccgatag aaaaagacgc tgcttaactt atggccttct gctatcagcg cctgcgcaaa
    21361 ctgaaaagca ctactcgcct gttgcgtacc gtatgctggc ccggtcacca cgatggcaaa
    21421 acgcattact tatcttgccc ctgaaaatca ccgctcttga actggcggat gtagagatag
    21481 acagtgtgtt tggagatgtt caggcggtca gcaacctggt tgatcgcatc tttaatatcg
    21541 aagatccctt tctcgtagag attcagcacg atctgacgat ttttggcgtt attagaaaca
    21601 ttgcgatcgg cattcacttc ttcgatggtg aactccagcg tttgggtaac cagatcttca
    21661 acagaagagg caaagttgac gcttgaaccg acatccgggg tttctggcgg cacaaaagtg
    21721 ctcataatct gcgagaaggg aacatcaaga ttcatattga tgcacagcag accaattaca
    21781 cgctgttcgc ggttacgaat cgcgatagtc agggacttca ttaatacgcc gcttttggcg
    21841 cgagtaaagt agcatttaga aacgctgcta tccgctcccg tcatatcgtg cagcatacgt
    21901 agcgccaggt cagtaattgg cgaaccaatc ttccggcctg tatgttcacc gttagcaatg
    21961 cgaatggctg aacattttag atcctgcaaa gagtgcaaaa cgatttcaca gtgggagcca
    22021 ataagcatcg ctaacccgtc caccaccgct tcgtaggatt tcagaatatc aaaatcggtc
    22081 tggtcgaaag gacgttgatc cagtaaatcc aactcactgg tttcgttggt taaaagcgac
    22141 ctggacatga aaaaaaacac tccttttcag gagcctgtcg ttaacttttc agggcaggct
    22201 cattaatgat gcgggtaact aaattaatac agcggaggtt ccgctttcca gcactaatta
    22261 tatccggcct gtaataaaaa aaccgccgcc tggtcaggcg gcggttctta atgcttattt
    22321 tttagcagaa tctgcggctt tcgcatcagc ttccggcttt gcatcagcct tcggcgctgg
    22381 tttcacatcc agcagctcta cgtcaaacac tagggtagaa ttcggtggga tccccggaac
    22441 acccgctttg ccgtaagcca gttctggtgg aataaccagt ttgatcttac cgcctttctt
    22501 gatgttcttc agaccttctg tccaacccgg gataacaccg tccagacgga aagaaagcgg
    22561 ttcaccacgg gtgtaagagt tgtcgaactc tttaccgtcg atcagcgtac ctttgtagtt
    22621 cactacaaca gtatcgctgt ctttcggtgc ttcgccttta ccggcttcta ctacctgata
    22681 aaccagacca gttgaagagg ttttcacacc tttctctttg gcaaatttct cgcggtactc
    22741 tttacctttt gcttcgttat cagccgcgtc tttttccatc ttcgcctgag cagaagactt
    22801 cacgcgagct tcgaatgctt gcagagtctg ttcgatctct tggtcggaga gtttgctctt
    22861 atcagcaaat gcatcctgaa caccagcgat cagctgatct ttatccagtt tgatgcccag
    22921 tttttcttgt tctttaagag agttttccat gtaacgaccc agcgaagcac ccagtgcata
    22981 agctgatttc tgatcgtcat ttttgaacga tgctttgctg tctgcagttg tagcaggttt
    23041 tgcagcttca gcagcaaaag tgattggtgc atgcagggca acggccattg tggtcgccag
    23101 tagcgttact ttaaacagtg atttcatcca tatctccagg atcggggcat ctcaccccag
    23161 ggttaactat tatcagaagg gtactataaa gcgttgtcga acaaatctac atacagacac
    23221 gccctattat catctatttt cagactcttt ttgtttaaat tagtttcgat gaccgcgaaa
    23281 tgagtgctgt ctcgggcagc aaagttaagt agaatccgcg gcgaccattc gacaaaagag
    23341 gtgaatcatg caggatttat cattggaagc acgcctggca gagctggaga gccgactggc
    23401 ttttcaggaa atcaccattg aagaactgaa cgtcacggtg accgctcatg aaatggagat
    23461 ggcgaaactg cgcgatcatc tgcgtctgct gaccgagaag ttaaaagcca gccagccgtc
    23521 gaacatcgcg tcgcaggctg aagaaacgcc accgccacat tattgaggcg taaaaaaagc
    23581 ggggattccc cgcttttttg tcaccttttc ggtattagtg gcaaccacaa ccgccgttgc
    23641 ctttaccgcc acagcagcct tcgccaccgt gttcatgacc gtgatcgtgg ccatgaccgc
    23701 cgcagcaacc gtcgtggtcg tgatcgtggt gatgatcgtg cgcgccgtga acgtggccat
    23761 gagccagttc ttcttcggtc gcttcgcgaa tcgccacaac ttcaacgttg aatttcaggt
    23821 tctgaccggc cagcatgtgg ttaccatcaa ccactacgtg atcgtcttca accgcagtga
    23881 tttcaaccgg taccggaccc tggtcagttt cagccaggaa acgcatacct acctgcagtt
    23941 catcaacgcc cataaatacg tctttaggaa cacgttgcac taggttttcg tcgtactgac
    24001 cgtaagcgtc gttcgcgcca acagcgacat caaatttgtc gccaacttca tgaccttcca
    24061 gcgccgtttc caggccagag atcagggaac cgtgaccatg caggtagtcc agcggcgcac
    24121 tcaccggaga ctcatcaacc aacacaccgt cttctgtacg tacctgatag gccaggctga
    24181 ccaccaggtc ttttgctact ttcatgatat ctcctgagca tgggaagaat agtggcgcag
    24241 attgtagcgg aattctgcag ccgtgtactc actagcttaa aaaaactcgg cgcatatcgc
    24301 tagtccggat gaaaaatccc gatcacttgc tcatctttgt gaacgtgatc gcgggcttct
    24361 ttgtctgctt ctcgcatctg atgtccgcac ttaacacatt caacaatatc aatattattt
    24421 tcgcgccaca tcgccattga atcctgcgcc tgacaggccg ggcattttgc gcccgctata
    24481 aaacgttttc ggattgccat ccttaccctc tactcaaatt catcccagcc gtccagctgg
    24541 cgtcgttctt gttgcatttc acgctggaaa atctcttcca gttcgcgtcg ggcttccctg
    24601 gcgcgagaaa tttgtacggt atcagcatgc atcgggatta actcccgcag cattcgcata
    24661 tccaggcggc gaaaatgcag ttgcgcccgc tgtgcctgat gcggatgcat gccaagcgtg
    24721 accagcgtct tgcgccccag ctctaacgca ctggagaatg tttcacggga aaactgcgtc
    24781 acccctgcct gtaataactc atgcgcttcc acacgtccgc gcgctcgcgc aagaatatgc
    24841 aaatgcggaa agtgctgctg gcatatttcc accagcttca tggtgtcttc cggctcgtta
    24901 caggtaatga cgatagactc agcggcctct gcacccgcag aacgtaaaag atcgacctgc
    24961 gtggcgtcgc cgtaataaac tttgtagcca tatttacgca tcaggttaac ggcgctgata
    25021 tcccgctcca gcacggtaat gcgcatttta tttgccatca gcaaacgacc aatcacctga
    25081 ccaaaacgcc cgaagcccac gacaatgacc tggggtttat catcgttgac ccacggtttt
    25141 tcgtcttctt cttccggtcc gttaaactgg cgggatagcc atttatccac cagcttcatc
    25201 agcaacggcg ccatctggtc gccctggaat aaacgttgtg aagaagcggt agaaaagagg
    25261 acaaaggcaa actcaccgcc ctgactcaac acgccagcaa actgcatccg ctctgagcta
    25321 cgcacgccat acaatcgcgc cagcagatac agcacgagaa ttttcaccgc caccagcaca
    25381 accacgctta tcactaccca caacagatgg gtataaagca ccccgaggtt gagcgacatg
    25441 ccgacagaga taaagaacaa accgagcagc aagcctttga agggatcgat agccgtttcc
    25501 agttcatggc gatattcact ttccgccagc agcacgcccg caataaatgt accgagcgcc
    25561 atcgacagcc ccagcgcatc cataaacaat gcggaaccca acaccagcag cagcgtcgcg
    25621 gcggtgaaca cttcccgcac gccagaagct gcaataaagc ggaataccgg acgcagtaaa
    25681 tagcgcccac caatcagcat gccgacaaac tccagcacct tcatgccgat cttcatccag
    25741 tcgaaatgtt cgtctgccga ccccgccaac aacggcacta acgccagtgc tgggatcact
    25801 gccagatcct gaaacagcag aaccgaaaac ccgagctggc cggattcgct gcgattcatc
    25861 cctttctcac gcatcaattg caacgccatt gcagttgaag acatcgcaaa gccaatgcca
    25921 ccgaccaccg ccgcctgcca ggcgaaatcc gtcagcatca ataatcccgc cagcaacgcc
    25981 gcgcttaaca gcacctgtgc cgcgcctacg ccaaaaatcg aacgccgcag ttgccaaagt
    26041 ttggagggat tcaactcaag gccgatgata aacatcagga ataccacgcc gagttccgaa
    26101 aagtggagga tctcatcgac gtcgctaata aaccccagcc cccacgggcc aattgcaatc
    26161 cccgccagca aatatcccaa cacagcgcca atccccagcc gcgaagagaa acagcactcc
    26221 tgcgagtaaa aaatcggaac cttccatcag cggcctcctg gagacagcgg atttgccaac
    26281 cagtcaccgt aggctctggc gtggctcgcc agctcctttg cgctttgccg tctcgcccag
    26341 taaataatga tgggacttaa ccaatgcatc cggcacatgc ccgccgccag ttcaaagggg
    26401 cgcagcacat cgctcatcgg gtagcgattc agcgcgtcat aacggtaagc actttccggc
    26461 tcgccggtgg taatcacgtt acgccagtac tttcccgcca gttggtttcc tcccggcccg
    26521 ctggcaaaac cacgacttaa tacccggtcc agccactctt tcagtagcgc cgggcagcta
    26581 taggtataaa gaggatgctg aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca
    26641 cgggggatat caataaaaaa atcgggatag tgcgcgtaaa ggtcgtgcac ggtaacattg
    26701 ctgagctgcg tggccggttt aagcagtacc cggtttgcca ccgagtcctg agattccgga
    26761 tgggcataca gcagcaaaac tttcgctggc tgagacatca tccccctccc ggtatggttt
    26821 ttgtgtatag tcgctgtttt gggctaccat tgcgcccggt gcggcagctc gcccatacat
    26881 tacattatca taatgataag ttaacatagt ctgaacatac ggcgccttat gattgttttc
    26941 tcctcgttac aaattcgtcg cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc
    27001 aaccccgggc agaaagtcgg cctggtgggt aaaaacggct gtggtaaatc taccctgctg
    27061 gcattgctga aaaatgaaat cagcgccgac ggcggcagct acacctttcc gggaagctgg
    27121 caactggcgt gggtgaatca ggaaacgcca gcgttaccgc aagcggcgct ggaatatgtc
    27181 attgacggcg accgtgaata tcgtcaactg gaagcgcagc tgcacgacgc caacgaacgt
    27241 aacgacgggc acgccattgc gaccattcat agcaagctgg atgctattga cgcatggagt
    27301 attcgctccc gtgccgccag cctgctgcac ggcctcggtt tcagcaatga acaactggag
    27361 cgcccggtaa gtgatttctc cggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg
    27421 atttgccgtt cagacttgct gctgctcgac gaaccgacta accacctcga tctcgatgcc
    27481 gttatctggc tggaaaaatg gttgaagagc tatcagggca cgctgatcct gatctctcac
    27541 gaccgcgact tcctcgatcc gatcgttgat aaaattattc atatcgaaca acaaagcatg
    27601 ttcgagtaca ccggcaacta cagttcgttt gaagtacagc gcgccacccg tctggcgcag
    27661 caacaagcga tgtacgaaag ccagcaggaa cgcgtggcac atctgcaaag ttatatcgat
    27721 cgtttccgtg ccaaagccac caaagcgaag caggcccaga gccgcattaa gatgctggaa
    27781 cgtatggagc tgattgcccc cgcacacgtc gacaacccgt tccgctttag cttccgcgcg
    27841 ccggaaagcc tgccaaatcc gttactgaag atggaaaagg tcagcgcagg ctatggcgat
    27901 cgcattattc tcgactcgat taaattgaat ctggtccccg gctcgcgcat tggtctgtta
    27961 ggccgcaacg gcgcaggtaa atcgacatta atcaaactgt tagccggtga acttgcgcca
    28021 gtcagcggtg aaattggtct ggcgaaaggg atcaagctcg gctacttcgc ccagcatcaa
    28081 cttgaatacc tgcgcgccga cgaatcacct attcaacatc tggcacgttt agcgccgcag
    28141 gagctggaac aaaaactgcg tgactacctc ggcggctttg gtttcctggg cgataaagta
    28201 accgaagaaa cgcgccgctt ctccggtggg gaaaaagccc gcctggtgct ggcattaatt
    28261 gtctggcagc gtccgaatct gctgctgctc gacgaaccga ctaaccacct tgacctcgac
    28321 atgcgtcagg cactcaccga agcattaatc gagtttgaag gcgcgctggt tgtcgtttcg
    28381 cacgaccgtc atttgctgcg ttccaccact gacgatctct acctggttca cgatcgtaaa
    28441 gtcgaaccgt tcgacggcga tctggaagat tatcaacagt ggttgagcga cgtacaaaag
    28501 caggaaaacc agaccgacga agcgccaaaa gagaacgcga acagcgccca ggcacgtaaa
    28561 gatcagaagc gtcgggaagc ggagctgcgt gcgcaaaccc agccgctgcg taaagagatt
    28621 gcccgtctgg aaaaagagat ggagaagctg aacgcgcaac tggcgcaggc ggaagagaaa
    28681 ctcggcgaca gcgaactgta tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa
    28741 cagcaagcca gcgccaaatc cggcctggaa gagtgcgaaa tggcgtggct ggaagcccag
    28801 gagcagcttg agcagatgct gctggaaggc caaagcaact gatggcgcag ataacgacga
    28861 ccgatgccaa tgaatttagc agcagtgctg aattcacccc tatgcgcggc tttagcaatt
    28921 gtcatctaca aactatgctg ccgcgtttgt ttcgtcgtca ggtgaaattc accccgtact
    28981 ggcagcggct ggagttgccc gacggcgatt ttgtcgatct cgcgtggagt gaagaccccg
    29041 cacaggcgaa acataaaccg cgtttagtgg tgtttcacgg gctggaaggc agtctcaata
    29101 gcccttacgc ccacggtctg gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga
    29161 tgcattttcg cggatgcagc ggtgaaccaa accgtatgca ccgcatttac cattcgggcg
    29221 aaaccgaaga cgccagttgg tttttacgct ggctgcaacg cgaatttggt catgcgccaa
    29281 cggctgccgt cggctattcg ctcggcggta atatgctggc ctgtttgctg gcaaaggaag
    29341 gcaatgatct cccggttgat gcggcggtga ttgtctctgc gccgtttatg ctggaagcct
    29401 gtagctatca tatggaaaag ggcttttccc gcgtttatca gcgttacttg ctgaacctgt
    29461 taaaagccaa tgccgcgcgc aagctggcag cctaccccgg aacgctgccg attaatctcg
    29521 cgcagttaaa atcggtacgt cgcatccgtg aatttgacga tctaatcacc gccagaattc
    29581 acggctacgc cgacgctatc gactattatc gtcagtgtag cgccatgcca atgctgaacc
    29641 ggatcgccaa accgacgctg attattcacg ccaaagacga tccgtttatg gatcatcagg
    29701 tgatcccaaa accggaaagt ctcccctcgc aggtggagta tcaactgact gaacatggcg
    29761 gtcatgttgg ctttattggc ggtacattac ttcatccgca aatgtggctg gagtcacgca
    29821 ttcctgactg gttaacaacg tatctggagg cgaaatcatg ttgattccgt ggcaagacct
    29881 ctcccccgaa acgctggaaa atttaattga aagctttgtg ttacgtgaag gcaccgatta
    29941 tggtgaacat gagcgtacac ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg
    30001 cggagaagcg gtgctggtat ggtcggagct gcacgaaacg gtcaatatta tgccgcgcag
    30061 tcagtttcgc gaataacctg caaccgtcgg cggacgagat aatagtgata gctaagtcat
    30121 ggagttacta tgtctgccaa acatccggtc attgcggtaa caggatccag cggcgcgggg
    30181 accaccacca ccagcctcgc gtttcgtaaa atattcgcgc agttaaatct gcatgcagct
    30241 gaggtggaag gcgacagttt tcaccgttac acccgcccgg aaatggacat ggcgatccgc
    30301 aaagcgcgcg acgccgggcg gcatatcagc tacttcggcc ccgaagcgaa cgacttcggc
    30361 ctgctggaac aaaccttcat tgaatacggt cagagcggca aagggaaatc tcgcaaatat
    30421 ctgcatacct acgacgaagc cgtaccctgg aatcaggtac cgggaacatt caccccctgg
    30481 caacctttac cagaacccac tgatgtactg ttttatgaag gtttacacgg cggcgtagtc
    30541 acgccacagc ataacgttgc gcagcatgtg gacttactgg tcggcgtagt gcctatcgtt
    30601 aaccttgagt ggattcaaaa actgatccgc gacaccagcg agcgcgggca ctcacgagaa
    30661 gcagtgatgg actcggtagt gcgttcaatg gaagactata tcaactacat cacaccgcag
    30721 ttttcccgca cccatcttaa cttccagcgc gttcccaccg tcgacacttc aaacccgttc
    30781 gcggcaaaag gtatcccgtc gctggatgaa agctttgtgg tgatccattt tcgtaatctg
    30841 gaagggatcg atttcccttg gctgctggcg atgttgcaag gctcattcat ttcccacatc
    30901 aatacgttag tggtacctgg cggtaaaatg gggctggcaa tggaattaat tatgctgccg
    30961 ctggtgcaac gattgatgga aggaaagaaa atcgagtaac tttgctatta tgccggataa
    31021 aatactatcc ggcttcacaa cgggatagtt aagtcacgcg gcaaccactt catacgagtg
    31081 agtaatattc accgcttttt ccagcatcaa cgccactgaa caatatttct cggcagagag
    31141 atcaaccgca cgcgcaaccg ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag
    31201 attaatgtgc gtaaacaggc gtggtgcctc ttcgcggcgt tcagaggtca attttacttc
    31261 acaatcgacc acatcctgac gccctttttg caggatcgaa accacatcga tggcactgca
    31321 accacccgcc gccatcaaca ccatttccat cggacttggt gctttatcgc ctgagttgcc
    31381 gtccattaaa atctggtgac cagaggcgga ttcgcccaga aaagttaacc cttcgaccca
    31441 cttcactcgc gcttgcattt ttgctactcc actgcgtcaa ttttcctgac agagtacgcg
    31501 tactaaccaa atcgcgcaac ggaaggcgac ctgggtcatg ctgaagcgag acaccaggag
    31561 acacaaagcg aaagctatgc taaaacagtc aggatgctac agtaatacat tgatgtactg
    31621 catgtatgca aaggacgtca cattaccgtg cagtacagtt gatagcccct tcccaggtag
    31681 cgggaagcat atttcggcaa tccagagaca gcggcgttat ctggctctgg agaaagctta
    31741 taacagagga taaccgcgca tggtgcttgg caaaccgcaa acagacccga ctctcgaatg
    31801 gttcttgtct cattgccaca ttcataagta cccatccaag agcacgctta ttcaccaggg
    31861 tgaaaaagcg gaaacgctgt actacatcgt taaaggctct gtggcagtgc tgatcaaaga
    31921 cgaagagggt aaagaaatga tcctctccta tctgaatcag ggtgatttta ttggcgaact
    31981 gggcctgttt gaagagggcc aggaacgtag cgcatgggta cgtgcgaaaa ccgcctgtga
    32041 agtggctgaa atttcgtaca aaaaatttcg ccaattgatt caggtaaacc cggacattct
    32101 gatgcgtctg tctgcacaga tggcgcgtcg tctgcaagtc acttcagaga aagtgggcaa
    32161 cctggcgttc ctcgacgtga cgggccgcat tgcacagact ctgctgaacc tggcaaaaca
    32221 accagatgct atgactcacc cggacggtat gcaaatcaaa attacccgtc aggaaatcgg
    32281 tcagattgtc ggctgttctc gtgaaaccgt gggacgcatt ctgaagatgc tggaagatca
    32341 gaacctgatc tccgcacacg gtaaaaccat cgtcgtttac ggcactcgtt aatcccgtcg
    32401 gagtggcgcg ttacctggta gcgcgccatt ttgtttcccc cgatgtggcg cagactgatt
    32461 tatcaccccg atatcaacta tgcacttcga caaacgctgg tgctatgttt gcccgtggcc
    32521 gttgggttaa tgcttggcga attacgattc ggtctgctct tctccctcgt tcctgcctgt
    32581 tgcaatattg cgggccttga tacgcctcat aaacgttttt tcaaacactt aatcattggt
    32641 gcgtcgctgt ttgccacctg tagcttactg acacagctgc tactggtaaa agatgttccc
    32701 ctgccctttt tgctgaccgg attaacgctg gtacttggcg tcactgctga gctggggcca
    32761 ttgcacgcaa aattgcttcc tgcatcgctg ctcgccgcca tttttaccct cagtctggcg
    32821 ggatacatgc cggtctggga accattgctc atctatgcgt tgggcactct ctggtacgga
    32881 ttgtttaact ggttttggtt ctggatctgg cgcgaacaac cgctgcgcga gtcactaagt
    32941 ctgctgtacc gtgaactggc agattattgt gaagccaaat acagcctgct tacccagcac
    33001 accgatcctg aaaaagcgct gccaccgctg ctggtgcgcc agcaaaaagc ggtcgattta
    33061 attactcagt gctatcagca aatgcatatg ctttccgcgc aaaacaatac cgattacaag
    33121 cggatgctgc gtattttcca ggaggcgctg gatttgcagg aacatatttc ggtcagtttg
    33181 catcagccgg aagaggtgca aaaactggtc gagcgtagcc atgcggaaga agttatccgc
    33241 tggaatgcgc aaaccgtcgc cgctcgcctg cgcgtgctgg ctgatgacat tctttaccat
    33301 cgcctgccaa cgcgttttac gatggaaaag caaattggcg cactggaaaa aatcgcccgc
    33361 cagcatccgg ataatccggt tgggcaattc tgctactggc atttcagccg catcgcccgc
    33421 gtgctgcgca cccaaaaacc gctctatgcc cgtgacttac tggccgataa acagcggcga
    33481 atgccgttgc ttccggcact gaaaagttat ctgtcactaa agtctccggc gctacgcaat
    33541 gccggacgac tcagtgtgat gttaagcgtt gccagcctga tgggcaccgt gctgcatctg
    33601 ccgaagtcgt actggatcct gatgacggta ttgctggtga cacaaaatgg ctatggcgca
    33661 acccgtctga ggattgtgaa tcgctccgtg ggaaccgtgg tcgggttaat cattgcgggc
    33721 gtagcgctgc actttaaaat tcccgaaagt tacaccctga cgttgatgct gattaccacc
    33781 ctcgccagct acctgatatt gcgcaaaaac tacggctggg caacggtcgg ttttactatt
    33841 accgcagtgt ataccctgca actgttgtgg ctgaacggtg agcagtacat ccttccacgt
    33901 cttatcgata ccattattgg ttgtttgatt gctttcggtg gtaccgtctg gctgtggccg
    33961 cagtggcaga gcgggttatt gcgtaaaaac gcccatgatg ctttagaagc ctatcaggaa
    34021 gcgattcgct tgattctgag cgaggacccg caaccaacgc cactggcctg gcagagaatg
    34081 cgggtaaatc aggcgcataa cactctgtat aactcattga atcaggcgat gcaggaaccg
    34141 gcattcaaca gccattatct ggcagatatg aaactgtggg taacgcacag ccagtttatt
    34201 gttgagcata ttaatgccat gaccacgctg gcgcgggaac accgggcatt gccacctgaa
    34261 ctggcacaag agtatttaca gtcttgtgaa atcgccattc agcgttgtca gcagcgactg
    34321 gagtatgacg aaccgggtag ttctggcgat gccaatatca tggatgcgcc ggagatgcag
    34381 ccgcacgaag gcgcggcagg tacgctggag cagcatttac agcgggttat tggccatctg
    34441 aacaccatgc acaccatttc gtcgatggca tggcgtcagc gaccgcatca cgggatttgg
    34501 ctgagtcgca agttgcggga ttcgaaggcg taatacaggc taaaccatcg gataaggcat
    34561 tcacgccgca tccgacatat tttgcctgat acgtcttatt tacgccccaa ccaccttcgc
    34621 caccgcgtgg gcgaaacgtt gcatcccttc atcgatatcc gcatcttcca ccaccagcga
    34681 cggtgctaaa cgcatcacat ccggtccggc attcagcacc attacgccaa cctctgcacc
    34741 cgcatacagg aaatcacgcg cccgaccttt gtagtgtggt ttcagctccg cgccaattaa
    34801 cagccccata ccgcgaatat cgctaaacac atcgtactgc tgatcgatct tctgcaaatg
    34861 gtcaacaaaa cgctggcgtt tcgcctgaat gccttccagc acttcagggg tattgatgat
    34921 atcaaacgcc gcgcccgcta ctgcacaggc cagaggatta ccgccgtagg tggaaccgtg
    34981 agaaccagga tgaaacgcag aagcaatttc cgccgtggtc agcatggcgc taatcgggaa
    35041 gccgccgcct aacgctttcg cagaagtcag aatatccggc gtcacaccgt agtacctgta
    35101 agcaaacaaa tcgccagtcc gccccatccc gcactgcact tcatcaaaca ccaataatgc
    35161 ctgatgttga tcgcacagct cgcgcaagcc ctgcaaaaac tctggcgtcg ctgccgtcac
    35221 accgccctcg ccctggatcg gctcaaccac caccgcacag gtgtgatcat ccatcaccgc
    35281 tttcactgca tggagatcgt taaagggaac gtggatgatg tctgccggtt tcggcccaaa
    35341 gccgtcggaa tattttggct gcccacccac cgaaacggta aacagcgagc gaccatgaaa
    35401 agcgttatgg aaggcaataa ttttggtttt gaacgggcta tgacgcacac aggcgtaatg
    35461 gcgtgccagt ttaaaggcgg tttcgttagc ttccgtgccg gagttcataa acaccacgcg
    35521 ttcggcaaac gtcgcctcaa tcagtttgcg cccaagacgc agcgccggtt cattggtgaa
    35581 aacgttactg atatgccaca gagtttcgcc ctgggttttt aacgcgttca ccagcgcagg
    35641 atggcaatgg cccaacgccg taactgcaat gccacccgcg aaatcgacat actccttgcc
    35701 ttgctgatcc cagattcggc tgccctgacc ttttaccgga ataaactctg ccggagcata
    35761 aatcggcagg atcacttcat cgaaagtcgc gcgtgtaatt gctgtttgtt caattgccat
    35821 ctcatgatca ccctgttacg cataaataaa tgtgaaatta taatcacaaa atatgcataa
    35881 aaaatcactg aatggcaatc ggaaatcaga gatgcaggaa attagccagc agttgatgtc
    35941 cttgttcgct aagaatactt tctggatgga actgcacacc ttccagatcc cactggcgat
    36001 ggcgaatccc cataatctct cgggtttcgc tccaggccgt cacgtcaaag cacgctggta
    36061 atgagtcagg ttccaccacc agcgaatggt agcgtgtcac ggtaagtgga tttgccagcc
    36121 cccgaaatac gccctcaccg ctatgtgtaa tcggcgaggt tttgccgtgc atgacctttg
    36181 cggcgcgcac aactttgccg ccaaatgcct gcgccattgc ctgatgaccg aggcagacgc
    36241 caagaatcgg caagcgcccg gcatagtggc gaataacgtc aagggagatc ccggcttcat
    36301 ctggcgtaca ggggccaggt gagatgacaa ttttttgtgg tttaagggcg tcgatatccg
    36361 ccagcgtcaa cgcatcgttg cgcttaacca gcacatccgc ccccagttca caaaagtact
    36421 ggtagaggtt ccaggtaaaa gaatcgtagt tatctataag caggatcatg gcggctccgg
    36481 tacgaaagaa ccgcgctatt ttactcagat tccccggctt cgcttaccac tttgctaaat
    36541 atcatctgca gtgcggtgag atcccccatt gccccactct gatttgcctg attccaggcc
    36601 tctttttcga taccctgcca gctcagttga tatccggcat gaatcgccag ttgctcgaag
    36661 aagatccgct gtgccagacc acttcccacc cggaagggat gcagcacgtt gatttcacaa
    36721 tagtaatgcg ccagccgctc gacgaacttc gctttctcca ggccaaccag ataaccttct
    36781 tcctccagat cctgcatcag ggcattgccc tctttttcga tataagcaaa gtggcagaac
    36841 ggcgtatcgc cctgataaat atcaacttca cgcagttgcc ctgcccagtc gaaaatatcc
    36901 tggtacagct ggcgatggat agtgcgcaaa tgcggtaaac cgcgcaccag cggaccaagc
    36961 tcaatggtcg cagcacgcag cgccgtcatt tcgtaagcgg cctgttccag ccgctgctgc
    37021 tggcggatgt tcagccggtt acgcatgata tcaaggccag gataaagata cggatcgcgc
    37081 ccttcgccga atttatcgct catagtgcct cctcagctct tcaaggcgcg ctaaagcctc
    37141 tgcggcagta agagtgacta aaggcatctc gacgccttca agacggcgac tggcctggaa
    37201 attacgatta cgctgaagct cccagagacg ggacttttgc ttatcggtga gtttcttcac
    37261 ttgatgcctc cctgaacgtg tccatttgcc acaagtataa gcggcaaatc cgggttacgc
    37321 cgggagagca gaagcgcggg caagattgcc cgcgcgagaa atcattacgg caggacttta
    37381 gcggaaagga taactaccgg ttttgacggc acattctggt acggaccaac atcatgagtc
    37441 ggcacctggg aaatcttatc ggcaacgtcc atgcctttca ccactttacc aaataccgcg
    37501 taaccgaaat cacgctgacc atggtcaagg aaggcgttat cggcaacgtt gataaagaac
    37561 tggctggtgg cgctgtcttt gtcagcggta cgcgccatcg cgatggtgcc acgcgtgttg
    37621 cgcaggccgt tatcggcttc atttttgatt ggcgggtttg gttttttctg ctgcatctgc
    37681 tcggtgaaac cgccgccctg aatcataaag ccaggaatga cgcggtgaaa ggtagtgttg
    37741 ttataaaaac cgctgttcac ataatcgaca aagttttgca cagacactgg cgctttttgt
    37801 ttatccagct ccagttcgat gttaccagct gaggttgtca acaatacgtg cgggtcccct
    37861 ttcgctgcca ttgctgcggg agaaagagca gaaagagcag aaagagcgaa aacagcagcc
    37921 atcgccgcca gggtcgattt gaacatgaga tttccttaac aaacagagca gaaaaaaagc
    37981 aagtgaagag attctaaata gccttcaaga acaaggccat ccctttacct aattttacgt
    38041 atctgaaata tatgtaacct gacgatcact ttttattccg tgatcaaaat cacctcttaa
    38101 aatgcaattt agcaaccgat tgcaataaaa catttaaaca gatcataaaa tcacctaaaa
    38161 tcgcccgctc gcggcatatc ccgctatgcc attttatttt tgaacgcagg ccaatcatga
    38221 ctaacagcaa tcgcatcaag ctcacatgga ttagctttct ctcctacgca ctgactggtg
    38281 cgttggttat tgtcaccggg atggtgatgg gaaatatcgc cgattatttc aatctgcctg
    38341 tttccagtat gagtaacacc ttcaccttcc tcaacgccgg cattttaatc tctatcttcc
    38401 tcaacgcctg gctgatggaa atcgtcccgt tgaaaacgca gttacgtttt ggctttctcc
    38461 tgatggtgct ggcggttgcc ggtttgatgt tcagccacag cctggcgctg ttctcgacgg
    38521 cgatgttcat tctcggggtg gtcagcggca ttactatgtc gattggtaca ttcctgataa
    38581 cacaaatgta tgaagggcgt cagcgcggtt cccgcctgtt atttactgac tccttcttca
    38641 gtatggctgg gatgattttc ccaatgatcg ccgcgtttct actggcgcgc agcattgagt
    38701 ggtactgggt ttatgcctgc atcgggctgg tgtatgtcgc tatttttatt ctgaccttcg
    38761 gctgtgagtt cccggcactg ggcaaacgtg caccaaaaac ggatgctccg gtagagaaag
    38821 aaaagtgggg gatcggcgta ctgtttctct ctgttgcggc gctgtgctac atcctcggtc
    38881 agttaggttt tatctcctgg gtgcctgagt atgccaaagg cctgggcatg agcctgaacg
    38941 acgcgggcac gctggtgagt aacttctgga tgtcatacat ggtcggcatg tgggcgttca
    39001 gctttattct tcgcttcttt gatttgcaac gcattctgac cgtactggct ggtctggctg
    39061 cgattctgat gtacgtcttt aacaccggaa caccagtaca tatggcgtgg tcaattctcg
    39121 ctctgggctt cttctccagc gcgatctata ccaccatcat caccctgggt tcacagcaga
    39181 ccaaagtacc gtcgccaaaa ctggttaact ttgtcctgac ctgcggaacc atcggtacta
    39241 tgttgacctt tgtggttacc ggcccgattg ttgaacatag cggtccgcag gcggcactgc
    39301 ttacagcaaa cggtctgtac gctgtcgtct ttgtgatgtg cttcctgtta ggtttcgtca
    39361 gccgtcaccg tcagcataac accctgacct ctcattaatt gctaatgccg gacggcacta
    39421 tcgtcgtccg gccttttcct ctcttccccc gctacgtgca tctatttcta taaacccgtt
    39481 cattttgtcc attttttgca caaacatgaa atgtcagaca attccgtgac ttaagaaaat
    39541 ttatacaaat cagcaatata ccccttaagg agtatataaa ggtgaatttg atttacatca
    39601 ataagcgggg ttgctgaatc gttaaggtag gcggtaatag aaaagaaatc gaggcaaaaa
    39661 tgagcaaagt cagactcgca attatcggta acggtatggt cggccatcgc tttatcgaag
    39721 atcttcttga taaatctgat gcggccaact ttgatattac cgttttctgt gaagaaccgc
    39781 gcatcgctta tgaccgcgta cacctctcgt cttacttctc tcaccacacc gccgaagagc
    39841 tgtcgctggt gcgcgaaggc ttctacgaga aacacggcat caaagttctg gtcggcgaac
    39901 gcgctatcac catcaaccgt caggagaagg tgattcactc cagcgccgga cgtaccgttt
    39961 tttatgacaa gctgatcatg gcaaccggtt cctacccgtg gatcccacca atcaaaggtt
    40021 ctgatactca ggactgcttt gtctatcgca ccattgaaga cctcaacgcc attgaatcct
    40081 gcgcccgtcg cagcaaacgc ggtgccgttg ttggtggcgg cctgttaggt ctggaagccg
    40141 ctggcgcgct gaaaaactta ggtattgaaa cccacgttat cgaatttgcc cctatgctga
    40201 tggcggaaca gcttgatcag atgggtggcg agcagctgcg tcgcaaaatc gaaagtatgg
    40261 gcgtgcgcgt tcacaccagc aaaaacaccc ttgagattgt gcaggaaggt gtcgaagcgc
    40321 gtaaaaccat gcgttttgcc gacggcagcg aactggaagt cgactttatc gtcttctcta
    40381 ccggtatccg tccgcgcgat aagctggcaa cccagtgtgg tctggacgtt gctccgcgtg
    40441 gggggattgt cattaatgat tcctgccaga cttccgatcc ggatatctac gccatcggtg
    40501 aatgcgcaag ctggaacaac cgtgtatttg gtctggtagc accaggctac aaaatggcgc
    40561 aggtcgccgt tgaccatatt ctgggtagcg aaaacgcctt tgaaggtgct gaccttagcg
    40621 ccaagctgaa actgctgggc gtagacgtag gtggtattgg tgatgcgcac ggtcgcacgc
    40681 ctggcgcacg tagctatgtt tacctcgacg aaagtaaaga gatctacaaa cgcctgattg
    40741 tcagcgaaga caacaaaacc ctgctcggtg cggtactggt gggcgatacc agcgactacg
    40801 gcaacctgct gcaactggtg ctgaacgcta tcgagctgcc ggaaaacccg gattctctga
    40861 ttctgccggc gcactcgggt agcggtaagc cgtctatcgg tgttgataaa ctgccggaca
    40921 gcgcgcaaat ctgctcctgc ttcgacgtca ctaaaggtga tctgattgct gccatcaaca
    40981 aaggctgcca cacagttgcg gcgctgaaag ctgaaaccaa agcgggtact ggctgcggtg
    41041 gctgtatccc gctggtcact caggtactga acgcggaact ggcgaaacag ggcatcgaag
    41101 ttaacaacaa cctgtgcgaa cactttgctt attcgcgtca ggaactgttc catttgatcc
    41161 gcgttgaagg cattaaaacc ttcgaagaac tgctggcgaa acacggcaaa ggctacggtt
    41221 gtgaagtttg taaaccaacc gtcggttcgc tgctggcctc ctgctggaac gaatacattc
    41281 tgaagccgga acatactccg ctgcaggatt ctaacgacaa cttcctcgct aacatccaga
    41341 aagacggcac ctactcggtg atcccgcgtt ctccgggcgg tgaaatcacc ccggaagggc
    41401 tgatggcagt gggtcgtatc gcgcgtgaat ttaatctcta caccaaaatc accggctccc
    41461 agcgcctggc gatgtttggc gcacagaaag acgatctgcc ggaaatctgg cgtcagctga
    41521 ttgaagctgg cttcgaaacc ggtcatgcct atgcgaaagc actgcgtatg gcgaaaacct
    41581 gcgtgggtag cacctggtgc cgctacggtg ttggcgatag cgtcggcctc ggcgtggaac
    41641 tggaaaaccg ctacaaaggc atccgtacgc cgcacaaaat gaagttcggt gtctccggct
    41701 gtacccgtga atgttcagaa gctcagggta aagacgtggg tattatcgcc actgaaaaag
    41761 gctggaacct gtatgtttgc ggtaacggcg gcatgaaacc gcgtcatgcg gatctgctgg
    41821 cagcggatat cgatcgcgaa acgctgatca aatatctcga ccgcttcatg atgttctaca
    41881 tccgtaccgc cgacaaactg acgcgtaccg cgccgtggtt agaaaacctc gaaggcggca
    41941 tcgattacct gaaagcggtg atcattgacg acaaactggg gctgaacgct catctggaag
    42001 aagagatggc gcgcctgcgt gaagcggtag tgtgtgagtg gactgaaacg gtcaatacac
    42061 cgtccgcgca gactcgcttc aaacacttca tcaacagcga caagcgtgac ccgaacgtgc
    42121 agatggtgcc agagcgcgaa cagcaccgtc cggcaacgcc gtatgaacgt atcccggtaa
    42181 ctctggtgga ggacaacgca tgagccagtg gaaagacatc tgcaaaatcg atgacatcct
    42241 gcctgaaacc ggcgtctgcg cactgttagg tgacaagcag gtagcgattt tccgcccgta
    42301 tcacagcgac caggtgtttg cgatcagcaa cattgatccg ttcttcgagt ccagcgtgct
    42361 atcacgcggg ctgattgcgg aacaccaggg cgagctgtgg gtcgccagcc cgctgaaaaa
    42421 acagcgtttt cgcttaagtg atggcttgtg catggaagac gaacagtttt ccgtcaaaca
    42481 ttacgaagca cgagtgaaag acggcgtggt gcagctgcgc ggttaatgtt ttaacgggag
    42541 gcgcaatgcc tccccttttt gcatggtcct gtaatgacct tcggtatatt gcaggacatt
    42601 ttttaaactt tttgttttat tttttgtttt tattttttaa aggataatca aatgtttaca
    42661 gacactatta ataagtgtgc ggctaatgct gcgcgcattg cacgcctgtc ggcaaataac
    42721 ccgctcggct tttgggtcag ctccgccatg gcgggcgcat atgtgggtct tgggatcatc
    42781 ctgattttca cgctcggtaa tttgctcgac ccgtccgtac gtccgctggt gatgggcgcg
    42841 acctttggta tcgccttaac gctggtgatt atcgccggtt ctgaactgtt caccggacac
    42901 accatgttcc tcacctttgg ggtaaaagcg ggcagcatca gccacgggca aatgtgggca
    42961 atcctgccgc aaacctggct gggtaacctg gtcggttccg tcttcgttgc catgctctat
    43021 agctggggcg gcggtagcct gctgccggta gataccagca tcgttcactc cgtcgcgctg
    43081 gctaaaacca ctgcaccggc aatggtactg ttcttcaaag gtgcattgtg taactggctg
    43141 gtttgcctag caatctggat ggcgctgcgc actgaagggg cggcgaaatt tatcgctatc
    43201 tggtggtgtc tgctggcatt tatcgcgtcc ggctacgagc actctatcgc taacatgacg
    43261 ctgttcgcgc tctcctggtt cggcaaccac agcgaagcct acacgctggc gggtattggt
    43321 cataacctgc tgtgggtgac gctgggtaat actttatcag gtgccgtatt catgggattg
    43381 ggttattggt atgctacgcc gaaagcgaat cgtccggttg cggacaaatt taatcaaact
    43441 gaaacagctg ccggttaatt actaaggggt ttttacgtgg atcatttgcc tatattttgc
    43501 caattacgcg atcgcgactg tctgattgtc ggcggtggtg atgtcgcgga acgcaaagca
    43561 aggttgctgt tagacgcagg cgctcgctta acggtgaatg cattagcgtt tattccacag
    43621 ttcaccgcat gggcagatgc aggcatgtta accctcgtcg aagggccatt tgatgaaagc
    43681 cttctcgaca cctgctggct ggcgattgca gcgacggatg atgacacgct taaccagcgc
    43741 gtcagcgaag ccgctgaagc tcgtcgcatc ttctgtaacg tagttgatgc gccgaaagcc
    43801 gccagcttta ttatgccgtc gattattgac cgctcaccgc taatggtagc ggtctcctct
    43861 ggcggcacct ctccggttct ggcgcgcctg ttgcgcgaaa aactcgaatc actgctgccg
    43921 ttgcatctgg gccaggtggc gaaatatgcc gggcaattac gcgggcgagt gaaacaacag
    43981 ttcgccacga tgagtgagcg tcgccgtttc tgggagaaat tgttcgttaa cgaccgcctg
    44041 gcgcagtcgc tggcaaacaa cgatcagaaa gccattactg aaacgaccga acagttaatc
    44101 aacgaaccgc tcgaccatcg cggtgaagtg gtgctggttg gtgcaggtcc gggcgatgcc
    44161 gggctgctga cgctgaaagg actgcaacaa attcagcagg cagatgtggt ggtctacgac
    44221 cgtctggttt ctgacgatat tatgaatctg gtacgccgcg atgctgatcg cgttttcgtc
    44281 ggcaaacgcg cgggatacca ctgtgtaccg caggaagaga ttaaccagat cctgctgcgg
    44341 gaagcgcaaa aaggcaaacg cgtggtgcgg ctgaaaggtg gcgatccgtt tatttttggc
    44401 cgtggtggcg aagagctgga aacactgtgc aacgcgggta ttccgttctc ggtggttccg
    44461 ggtattaccg cagcttctgg ttgctctgcc tattcgggta ttccactcac gcatcgcgat
    44521 tatgcccaga gcgtacgctt aattaccgga cacttaaaaa ccggtggcga gctggactgg
    44581 gaaaacctgg cggcagaaaa acagacgctg gtgttctata tggggttgaa tcaggccgcg
    44641 actattcagc aaaagctgat tgaacacggt atgcctggcg aaatgccggt ggcaattgtc
    44701 gaaaacggaa cggcagtcac gcagcgcgtg attgacggta cgctcacgca actcggtgaa
    44761 cttgctcagc aaatgaacag tccatcgcta attattattg gtcgggttgt tggcctgcgc
    44821 gataaactga actggttctc caatcattaa tttaacccgg gccagagaat tctggccttc
    44881 ttaatgactc tttttatata ttcagcaaat aaaacataac cccttaataa atattatctg
    44941 ctaatgggtt atattcaccg ttacagcgac ttacaaaatt aagtcattcg ctcactgacg
    45001 ccaattaaat attcctgtat cgttcttaac aagcagtctg cttatattca tataatcaat
    45061 gaatattaat taatatataa tacataggga atgtaaatga acaaatttat taaagttgca
    45121 ctggtaggtg cagtactggc tacgttaact gcatgtactg gtcatattga aaaccgtgat
    45181 aagaactgct cttacgacta cctgctgcac ccggcaattt ctatttctaa aatcattggc
    45241 ggttgcggtc ctactgcaca gtaagtctct gacagaaatc ggctaacacc ggtttaattg
    45301 cccgacgccc tctggatttt ccgagggcgt atttgtttct atgtccttct tgcccccgcc
    45361 cgtaaaaata ttttcacttt aaattcaatt ttataactac atccattagc cacagcgcga
    45421 tcctgtgcga aattttgtga tcttcctcca cattacataa cataatatgt tgttatattc
    45481 atcatgcatt gtcatgttac cttttaaatg actgcaaact ctcccctaca acgtattgga
    45541 caagaaaaag gtatcgctat gggaagccaa gaactccaac gcaagctcgg attttgggcc
    45601 gttcttgcaa tcgccgtcgg gacaaccgtc ggctccggta tttttgtatc tgtgggtgaa
    45661 gtggcaaaag cagcgggcac gccgtggctt acggtgctcg cgtttgtcat tggcgggtta
    45721 attgtgatcc cgcaaatgtg cgtctatgcg gaactatcca ccgcttatcc ggaaaatggc
    45781 gcagattatg tttatctgaa aaatgccgga agccgaccgc tggctttcct ctccggctgg
    45841 gccagcttct gggccaacga tgcgccgtca ttgtcgatta tggcgctggc gattgtcagc
    45901 aatcttggct ttttaacgcc tatcgatccg ttgctcggta aatttatcgc cgccggatta
    45961 attatcgcct ttatgttgct acacctgcgc tccgttgaag gcggcgcagc gtttcagacg
    46021 ctaattacca tcgccaaaat tatcccgttc actatcgtca ttggccttgg gatcttctgg
    46081 tttaaagcgg agaattttgc cgcccttacc accactgcga ttggcgcaac gggcagcttt
    46141 atggcgctgc tggcggggat ctctgccacc agttggtcgt ataccggcat ggcctctatc
    46201 tgttatatga ccggcgaaat taaaaacccc ggaaaaacca tgccacgagc gctgattggt
    46261 tcctgtctgc tggttctggt gctctacacc ctgctggcgc tggtgatttc cggcctgatg
    46321 cccttcgaca aactcgccaa ttctgaaacg ccgatttccg acgccctgac ctagatcccc
    46381 gcactcggca gcaccgctgg gctctttgtt gccatcacgg cgatgatcgt cattcttggt
    46441 tcgctttcca gctgcgtgat gtaccagccg cggctggaat acgcgatggc gaaagacaac
    46501 ctgttcttta aatgcttcgg ccatgtgcat ccgaaataca acacgccgga tgtctccatc
    46561 atcctgcaag gggcgctggg gatcttcttc atcttcgttt ccgatctcac cagcctgctg
    46621 ggttatttca ccctggtgat gtgtttcaaa aataccctca ccttcggctc catcatctgg
    46681 tgtcgtaaac gcgacgatta caaaccgctg tggcgtactc cggctttcgg gctgatgacc
    46741 accctcgcca ttgcgtcaag cctcattctg gtcgcctcaa cctttgtctg ggcaccgatt
    46801 cccggcctta tctgcgccgt catcgttatt gctactggtc tgcctgctta cgccttctgg
    46861 gcgaagcgta gccgccagct caacgctttg tcgtaatgtt atctgaagaa aataaaatgt
    46921 tggatattga taaaagcacc gtggactttc tggtcaccga aaatatggtt caggaagtgg
    46981 aaaaagttct cagccatgac gttccgctgg tgcacgccat cgtggaagag atggtgaagc
    47041 gcgacattga tcgtatttat ttcgttgcct gcggctcgcc actcaacgcg gcgcaaacgg
    47101 cgaaacatct ggcggatcgc ttttccgatc ttcaggtcta cgccatttcc ggctgggagt
    47161 tctgcgataa caccccgtat cgcctcgacg atcgttgcgc ggtgattggc gtttctgact
    47221 acggtaaaac ggaagaggta atcaaagcgc tggagctggg ccgggcctgc ggcgcactca
    47281 ctgcggcgtt caccaaacgc gcggacagcc cgattacctc ggcggcggaa tttagcattg
    47341 attatcaggc cgactgtatc tgggaaattc acctgctgct ctgctacagc gtggtgctgg
    47401 agatgatcac ccgcctcgcg ccgaacgcgg aaatcggcaa gatcaaaaac gatctcaagc
    47461 agttgccgaa tgcgctcggt catctggtac gcacctggga agaaaaaggc cgccagcttg
    47521 gtgaactggc cagccagtgg ccgatgattt ataccgttgc tgcgggtccg ctgcgtccgc
    47581 tgggttacaa agaaggcatt gtaacgctga tggaatttac ctggacgcac ggctgcgtga
    47641 ttgagagcgg agagttccgc catggcccgc tggagattgt cgaaccgggc gttccgttcc
    47701 tgttcctgct cggcaatgat gaaagtcgcc acaccaccga acgcgccatt aactttgtta
    47761 aacagcgtac tgacaacgtg atcgtcatcg attacgccga aatttcgcaa gggctgcacc
    47821 cgtggctggc accgttcctg atgttcgtgc caatggagtg gctctgctac tacctgtcta
    47881 tttacaaaga tcacaacccg gatgaacgcc gctattacgg tggtctggtg gaatattaat
    47941 ccctctcccg gcccggcaac gggccggatt tacgcaagga gttacccgat gaaaacaggt
    48001 atgtttacct gcggccacca gcggctgcct attgaacacg catttcgtga tgcaagcgag
    48061 ctgggttacg acggcatcga aatttggggc ggtcgcccgc acgcgttcgc gccggactta
    48121 aaagcgggcg gcatcaaaca aatcaaggcg ctggcgcaga cgtatcagat gccgattatc
    48181 ggttacacgc cagaaaccaa cggctatccg tataacatga tgctgggcga tgaacatatg
    48241 cgtcgtgaaa gcctggacat gatcaagctg gcgatagata tggcaaaaga gatgaacgcg
    48301 ggttatacgc tgatttctgc ggcccacgcg ggctatctca cgccgcctaa cgttatctgg
    48361 gggcggctgg cagagaactt aagcgaactg tgtgagtacg cggaaaacat cggcatggat
    48421 ctgatcctcg aaccgttaac gccgtatgaa tcgaacgtcg tgtgtaatgc taatgatgtg
    48481 cttcatgcgc tggcgctggt gccttcgccg cgcctgttca gcatggtcga catttgcgcg
    48541 ccgtatgtcc aggcggaacc ggtgatgagt tatttcgaca aactgggcga taaattacgt
    48601 catctgcata ttgtcgacag cgacggggcc agcgacacgc attacattcc tggagaaggc
    48661 aaaatgccgc tgcgggaact gatgcgcgat attattgatc ggggctatga gggttactgt
    48721 acggtggagc tggtgacgat gtatatgaac gagcccagac tctatgcccg ccaggcgctg
    48781 gaacgctttc gcgcgctgct gccggaggat gagagatgaa aaccctggcg acaatcggcg
    48841 ataactgcgt cgatatctac ccgcaactga ataaagcgtt ttctggcggt aatgcggtca
    48901 atgtggcggt gtactgcact cgctacggca tacagccggg atgcattacc tgggtgggtg
    48961 acgatgacta cggcacaaag ctgaagcagg atctcgcccg catgggcgtc gatatcagcc
    49021 atgtccatac gaaacacggc gttaccgcac aaactcaggt ggaactgcac gacaatgatc
    49081 gcgtttttgg cgactacacc gaaggcgtga tggccgactt tgccctgagt gaagaggatt
    49141 acgcctggct ggcgcagtat gacattgtgc acgcggcaat ctggggacat gcggaagacg
    49201 cattcccaca gctgcacgct gcgggcaaac ttaccgcttt cgacttctcc gacaagtggg
    49261 acagcccgct ctggcagaca ctggtgccgc atctcgattt tgcctttgcc tccgcaccgc
    49321 aagaagacga agcgctgcgt ctgaagatga aagcgattgt tgcccgtggc gcaggaacag
    49381 tgattgtcac gctgggtgaa aacggcagca ttgcctggga tggcgcgcag ttctggcgtc
    49441 aggctcctga accggtgacg gttatcgaca ccatgggtgc cggagattcg ttcattgccg
    49501 gattcctttg cggctggtct gcggggatga cattaccgca ggcgatggcg cagggaacgg
    49561 cgtgcgcggc gaaaaccatt cagtaccacg gtgcctggta ggtataacgt tggcgtgagc
    49621 atcttcacgc caacgtgctg ttacttgccg gaaaacgacc ctataatccg agtaattcat
    49681 tctttatttc agggtcgatt atgtcagcta cggaccgcta ctctcatcaa ctcctctacg
    49741 ctaccgtccg ccagcggctg ctggatgata tcgcgcaggg ggtttaccag gccgggcaac
    49801 agatccctac cgaaaacgag ctttgtacac aatataacgt cagccgcatt accattcgca
    49861 aagccatcag cgacttagtg gcagacggcg tactgatccg ctggcaggga aaaggcacct
    49921 ttgtacaaag ccagaaagtt gaaaacgccc tgcttactgt cagtggtttt accgattttg
    49981 gcgtctcaca aggcaagtcg acgaaagaga aagtgatcga acaggaacgg gtcagcgccg
    50041 cgccgttttg cgaaaagctg aacatccccg gaaacagcga agtgttccat ctctgccggg
    50101 tgatgtatct cgataaagag ccgctgttta ttgatagttc atggatcccg ctgtcgcgtt
    50161 atcctgactt tgatgagatt tacgtcgaag gaagctccac ctatcagtta tttcaggagc
    50221 gttttgacac gcgagtggtc agcgacaaaa agaccatcga tatctttgcc gccacccgcc
    50281 cgcaggcaaa atggctgaaa tgcgaactgg gcgaaccgtt gtttcgcatc agcaaaatcg
    50341 cctttgatca gaatgacaaa ccggtgcacg tctccgaact cttctgccgc gccaatcgca
    50401 tcaccttaac gattgataat aaaagacatt aaccgtaggc cggataagat gcgccagcat
    50461 cgcatccggc gatgctggcg cactgaattt tacatcccgc acgttcccct caccctaacc
    50521 ctctccccaa aggggcgagg ggaccgttca gtgcggaatt tagcttgatt gccgaacggt
    50581 gctatccagc attaaatact cgcaatactc tcacgcaaaa tccgcagcac cgtctcttca
    50641 ccgctgcgat tcgggttaat gcgaatcgca caatgttcga gttgtggatt cgcctggcga
    50701 aacgttcctg aaagtcgata gaagagcggc gggatttcat atttcgactc tgcaccgacc
    50761 gggtaaggca acgcgccacg cttttgcgct tcttccagca ccctggcggc aatcagctga
    50821 tgaaactcga caatcaacac cttcgactgc gcattggcaa tgacggcgct tttcacttcc
    50881 gccaccgcgc cgccgttaag caaagccagc aaccgttcgg atactcccgc ctgcaccgca
    50941 tgcatcactg gcgcgaacac cagaccacgc aagacttcca gcgcctgtga gccctggatc
    51001 tggctaccgc cggagtaaag cgtggcgcga atccgactaa taacatcggc atcgccaacc
    51061 actgcaccaa cgccccccgg cccaaacagc ttgaagcagg agaatgtcga gacattcgcg
    51121 ccgcattcac agccgattcg cgctaccttc atcaccgcat agttatcatc ggttaacgct
    51181 ggaacacctg ccgcgcgcaa cgttgccagc acatctgcca gcacatagct gtcctgcggc
    51241 tgctggcgcg tatgctgcac cagcgcggca tcaggttgtt gctcgtcgac gacctgcttc
    51301 agtgccgaca ggtcattgaa atcagcagta ataagcgtca gccccatctg ctcaataata
    51361 acccgtgtcg tcgggtaaac aggcgcgtca tgcaccagaa gacgctgccc cggtttgagc
    51421 aaagccgcca gcccggcgcg aatcgcgcca gtccccgcgc cctgcaccag cgccgcggcc
    51481 tgtgcgtgaa atgcatcagc cagcacctgc tccacccgtt gggtaatacg cggttgattc
    51541 agacttggag ttaaccctaa atcaccgcca gcaagaaact cgctgcctgg aaaatggcgg
    51601 caaatagtgt ccaccagcgc aaactgcttt tgctgcgctt cagcaagagt caggctttgc
    51661 aaaggaaacg tcttcatagg tgccttcctt aagccggaac gaacaggcca agccagtaaa
    51721 gaacattcag cagaataccg gtgatcatta ctgccacgac cggtgccgcc attttctgta
    51781 ccggacggcc cagggattcg ttgaggaagt aaatggcaac cgcgatagag aatccggtat
    51841 aacccgccat cttaattgct gcgaaaatcg aaccgaccag cagcgccact tccatcagca
    51901 tattcatggc gttacggatg ttatccgacg cattacgcac cgacgggtag cgtcccagcc
    51961 atttgccgat cgaacgaagc agcaagactt ccgccgaaat aaccactgcg cctaataccg
    52021 ctgcaaccat cggattcggc gagagatagc ccaccgcata aacaaaggta aagcccgcaa
    52081 ctgcatacac acccgttgct aaggcggtgg tggcaatcaa cggcacaaac cccagtccgc
    52141 gcataaattc tgccagagcc gcctgattaa tcagcgtttg cgattgttcc ggcgtcacgc
    52201 ctgcggaata cgctttctcc agtgtgaaga tcgaaacttc gctaccagca aaaatcttca
    52261 tactggcaac ggcggcaatc aatgctccca cgatggcgat ataaggtaag tttttgataa
    52321 tccgcgacgt gcgctcttca aacaccgaaa gcccgctggc atcgatgtca ttttcatcac
    52381 gatgacgcag gtcgtgagtt atcgcgatcc ccagcagcat caccatgcca ataaagattt
    52441 cgatggattc agggttaaga tgtgggaaat agcgcacgac taccacacgg gtcatcagaa
    52501 caaccacggc ggcgaccaga ctttgcttcc agccaaactg gtagaaaatc gccaccagcg
    52561 ggaacagtgc aaaagcagaa accaccggcg agcttaattc cccgaggcta cctaatacat
    52621 ccaccggcag cgcggtcagc agctggttaa ctggcaacag gcaagtaagg atcaacacgc
    52681 cccagatagc gccaagacca aacgccatca ggctgtttat cgccagtacg ccgaggatat
    52741 cggtaggaag aaagagtaac catgcgttga gcagcccggt tttcagggta aaagagatcc
    52801 ccaccgaggc cacaaaacca atgctcagac caaaagcgat actccccgcc tcgcgacggt
    52861 tcatatagcc ttcaatcagt tgcggcagga tcgggtggat gccgtcatga aaaacagccg
    52921 ccgagcgatg cgccagaagc gatgtcatac ccgtcaggca cgccaccacg ataatctgaa
    52981 tatacagatc catagtcgtg ccttttattt cagatggtta atcaacatcg gaatcgcgtg
    53041 ttcgacgtgc tcaacggaaa ggccaaacgc cactttccct tcagcgatca ttttggcgat
    53101 atgctcgtct ttcgctttaa tgcctggttt ggcaatggtg caacttttgt tatagccaat
    53161 caccgcgatg gcaatcgaca atgccgcgcc cgcaccggta ttacacgcgc caatgtaata
    53221 atctagttgc ccggatttca ccttcattgc cgcttccatg tcgttgtgaa tgaaaacttc
    53281 aaaacagcca ggagccgtcg cttcaatggt ttttttaatc tgctcacgct gtaagcctgc
    53341 aacgccaatc tttttcatga gtctgtcctt attggaaaaa ttgagaggga ttttcacgta
    53401 acatcacatc cacatcggcc tgactgaatc ctgactggcg caattgcgga ataaaggtgg
    53461 tcaataaata gtcatagcca taaccaccat tggcttttaa atgggagcgg cgcgtaatat
    53521 ccatcgacag catgacgcgg ttcagcaacc cacggtcacg tagcgcgtgg agcatcgcaa
    53581 tgcgcttttc gtccgggtag taactgttct taccaatggt gtcgaactgc acgtacgcgc
    53641 cgagatcgat catcttcaaa atgttgtcga ggttgtcttt cagatcgcag tgaccaacgg
    53701 tgacgcgcga aagatcaact ccgtgggctt gcaacagagc cagttgttcc agccccatgg
    53761 tgctgaacga cgtatgcgtg gagatcgggc gtccggtctg gttatgcgcc agcgcagcgg
    53821 caataaatac cttctcttcc agcggcgtaa tctttccttc gctggagccg atctccgcga
    53881 tgatcccggc tttcagctcc gtgccatcga taccctgttc aatttcatcg accatctcct
    53941 gcgccagttc ctgcacgctg cgggtcacca catgttccgg gaaaaacgca tcctggtaat
    54001 aaccagtaca ggccaccacg ttgatcccgg tttcgcgcat tacatcgagc ataaattgcg
    54061 cattgcgccc catgtaacgg ttggtcatct caatcacatt acgtacaccc cgggtcatca
    54121 ggtcgttcat ctcctggcaa atgaacgcat actgatcaag gcggcagtcc acgttgtttt
    54181 taaagccgga gagatcaata tgcagatgct catgggccag ggtgtaaccc gtcggatcaa
    54241 aactcatagg gtgtctcctg caaaccggag ggaggaaaga aaagagcgac cattttgtgg
    54301 cggtggcgca cggaaaaatt cacacacggt agctcccaca tcagaaagcg tggtgcgcac
    54361 accgagctgc gtggcgacca gcccttgctg ataaaccagc actggcacca cttcacgggt
    54421 atggtggctg tgaccaatgg tcggatcgtt accgtgatcc gccatcacga ccaggcaatc
    54481 atctggctgc atcgcctcaa caagccgggc aaggttacgg tcaacgacct gcaaacgttc
    54541 ggcataacgt gcgacgtctt ctgcatgacc agcgaggtcg gtttcctgga tattgatgca
    54601 aataaacgcc gtcggatggg tgttaaattc gtcgagggtg atatccataa tccgctggct
    54661 atccaccagg ttttgccagc tcacaccata aggattgctg acgatatctg ccaccttacc
    54721 caccagcacg gttggcacgc ccgcttcata cagtttttgc ggcacctgca ctttttcatc
    54781 aacaccgtag cccatatgca cgacctggaa gccgttgtca taagcgccag aacgcggcgc
    54841 attaatacca ataaagcgcc cttctttgct ttctgcggca tcgagaatac gctgactgtt
    54901 ggttaacagg ccgccaaatg taatgacccg accgacctgt acctggtcac gcacgatacg
    54961 accaattttg attgcgtcgt caaaagagat cacagagaga ttggcggtaa tgttatagac
    55021 ttggcctaaa tccgcctcga gattatcgcc aatcgcaacc gcctgattga ccgacagaaa
    55081 ttgcagatca tcgccacggc gctccacctg ccagccagcg gaaactaatg cctgctcaac
    55141 acggtcaatc acatcgcgaa aaggcatccg cagcggcggt aacgggcgcg tgcctaaaat
    55201 ttcctgatgc cccataaagg tatcgccacc ttcatgttgc agctctgcca cgccccaggt
    55261 tgcggaatct gacggctgca tatcgcctgg cgcataaccc aatgtgttga ttagccccag
    55321 cttctccagc gttggtagct gcaaatgcgg caactggctc aggatgtgac cacaggtatt
    55381 cgccccggca tcttgcgggc gcaccagcgt gacatctttc attgccccta cgccaaagct
    55441 atcaatcact aacaccacaa atcgcgccat ttatgcctcc agagggttgc cgaggctgtc
    55501 ataacgacca actatttctg gttcgccgcg ctgaataccg gacaccagca ccacatcgct
    55561 acgggtgaca aaaatctgcg tgcgaaaaca gagcaccact gcactgctca ccggatactc
    55621 gcctgccagc ggcagggtgt agtcgatact gctgtcatcc acagttttga gattggtttc
    55681 agtaatcttt tgattttctg gcgtaaacac cagcgcatgt tgcgcatgac cacgacggta
    55741 gtaaccgccg ccgtagcagt agctgtcgcc acggaaatga tgggagattt cacttaacca
    55801 gagcatcgcg atacgttcag gctgatcgcc ctgctggttt gccggaatag taccggtcag
    55861 cgcatgaccg ggttcggcat gagtcgcacc gtattccgcc agcaatggca gcgaagtgca
    55921 gctggtcgct gaaggtacgt tcagttgctc aatcgcaata ccagatttcg ccagttgatc
    55981 ccgtgcttgt accagcgtgt gaagattcgg tgttggcaaa actttcccag cagcctcatc
    56041 ccaaagcagg caagggaaat gggttagtcc ggctaaatgc agccccggca gtttcttaat
    56101 ttcagcaatc acctcgtgca gcgaatgctg tacaaaaccg ctctcctgcc ccggataaag
    56161 aaaatcatcg tcgctataaa ctttgagcag cacaggctgg acccgtccgg ccttaaccgc
    56221 tgccgcagaa acttcccgcg ctttgtcgag agtaaacacg gtgatgatgt cggtgccctg
    56281 ttcaacggcg tcagcaacct gatgacaagg gatttgtacc agatgcccct gatgcgccac
    56341 tggcaaacca gcgcggcgca tgactcgcgc ctctttgtaa tccaccgcca caatgccgct
    56401 gtagcctaat gccagcaatt tttccgccag ccacggatta cgaccaaatt gcttggtcat
    56461 cagatacagt tcaatgccgt aaagccgcgc cgtctcaatg agccgcttac cgttttccag
    56521 tatctgatcc acgtcgatca cccagctgtc cggggcgatt ttgccctgct gccacaaact
    56581 tagtgcggcg gaaatcagcg ccgggttctg gcgtttcaat gcttctacaa acatcttgtt
    56641 gcctcgtttc tgttccttga attaaatatt catttttttg aatatttagg ttgtgcgacg
    56701 tggatttgtc gggagcttca aacctcttcg ttagcagcca tccataatcc ataaagattg
    56761 gccagaaaat agccttcttc acacgggtta acttccagag cgaattcctt caacaactcc
    56821 tgatgcaatt gcacaacggc ttgccagtgg ctggattgcg ccagttccgc cagcaactcg
    56881 ttatccagcg gctctatttc ttcaccacgg cgactgcgca tcagtgcact cgccatatgt
    56941 gtcatcgcca tcgttccttg ctcactgcgc accggcagat ggcactcttt ttccagtacg
    57001 ttgacgacct gcatcatgcc tttgcagacg tccttatcaa taacgcctgc ctcgcaaagc
    57061 aggttgagtc tggtttccat atcagatgcc tttcgtttaa taactgggtt cctgttcccc
    57121 actcaccacc cgttgttgat gggcaagcag ggcgtgttta tctacaacag cacgcagcaa
    57181 ttgttgcatt gggtaatcat cgactcgcgt caggaccacg gcttcggtgg cctgtaaaaa
    57241 tcgcggatcg tctgttagcg gcgtcgcctc taatcccagc atggtcagtt cgttttccgc
    57301 caccacgttc cagatcaccg catcgacatc gcctttaaca atgcgttgta aactctcgtg
    57361 ataagagaga tcgactcgtt ccacatcact atcgccaaaa aagacatcgg tcatgatttt
    57421 ctgatccgcc gaacgactat cgagacctac tcgcttaaca tttccagact cacctttgcg
    57481 gcatatcagt tgatgttcac caacatacgt atgcgggcca agctcgaggg cgatgcacaa
    57541 attgttttgg ctgagataac tttccgccgc caggcgggaa actactgcca tgtcatagac
    57601 accgttaagt aaacactcca cgcgaatatc cgcgccgcgc atatgcgcat agtaaaaagg
    57661 aatgccatca aactgggctt tcaatccgct cgctaaacct tcgtacaagc gggtataggg
    57721 caaaggcatc gcacatacca cgttgttgat atccacatga ctcagcaatg ctttgttatc
    57781 catctcgaca agataactgc cattgcgccc acggcgttca atccgtatcg ctccgcttga
    57841 ttccagcgtt ttcaacgcgg cctgcgtcag gccaaccgat gaacggcatt cagttgccag
    57901 ctcatctatg gttttcagtc gattaccgca tttttcaccc aacaaataac gggccagtgt
    57961 cgtgatgacg acgccttctt ttttgataaa cgttcgacgc ataatatatt ttcaataaaa
    58021 tgaatattta tatcttcatt aaaatgaaga taagtggcga cgacgagatg tggaaacggc
    58081 gaggcgcttc acattttttc gggttgttgc gaaaagagga taaaaaaacc gggtttcccc
    58141 ggtttcagag tgatgacgaa gtgatttatt cgacaatgcg agcaaagggt aattcccact
    58201 ggcgcttgcc tgctcccctc accctaaccc tctccccaaa ggggcgaggg gaccgtccga
    58261 gcgttttgat attacggctt cgccacaaaa ccaatcgctt cgtacaccgc tttcagcgta
    58321 cgggaagcgt gtacactggc tttttctgcg ccgtctttca tcacctgttg caggaaggct
    58381 tcatcgttgc ggaaacggtg atagcgttcc tgcaattcag tcagcatacc ggaaacggca
    58441 tcagccactt cacctttcag atgaccatac atcttgcctt cgaactgttt ttccagttct
    58501 gggatgctct ggcccgttac cgcagaaagg atatccagca ggttggaaac gcccgctttg
    58561 ttctgcacat cgtagcgaac taccggcggc tcgtcggagt cagtgaccgc gcgtttgatt
    58621 ttcttcacta ccgatttcgg atcttccagc aggccgataa cgttattgcg gttatcgtca
    58681 gacttggaca tcttcttggt cggctccagc agcgacatta cgcgcgcgcc agatttcgga
    58741 ataaacggct ccggcacctt aaagatctcg ccatacagcg cgttgaaacg ctgggcgata
    58801 tcgcgactca gttccaggtg ctgtttctgg tcttcaccca ccggtaccag attagtttga
    58861 tacagcagga tgtccgcagc catcagcacc ggatagccaa acagaccagc gttgatgttc
    58921 tcggcataac gcgcagattt atctttaaac tgcgtcatgc gactcagttc gccgaagtag
    58981 gtatagcagt tcagtgccca gcctaactgc gcatgttctg gtacgtggga ctgaacaaaa
    59041 atggtgcttt tctcaggatc gataccacaa gccagataca aggccagcgt atccaacgtc
    59101 gctttacgca gcttctgtgc atcctggcgc acggtgatcg cgtgttggtc aacgatacag
    59161 taaacgcaat ggtagtcatc ctgcatgttt acccactgac gcagcgcacc catgtagtta
    59221 ccaatggtca attcacctga gggctgtgcg ccactaaaaa cgatgggctt agtcattttt
    59281 cgattcctga ttttcgctat gcggaagccc taatgcgggc agaaggtcat ttatagactg
    59341 ataaattaca tcaggctggc tgagatcgat agcctcgccg tagttgtatc cgtaggttaa
    59401 gccaactgat gggcaacctg ccgcttttgc cgcctgaata tcattccgtg agtcgccgac
    59461 aaacagcatc tgttgtgggg caattcccat ccgctcagcc accagtaaca gcgggtccgg
    59521 atgcggtttt ttgttttgca catcatcgcc gccgataacc acgctaaagt atttggcaat
    59581 atctaaggct tcgagcagcg gcgcgacgaa cggcgtcggt ttgttggtga ccaagcctag
    59641 cggcaggcct ttagcctgca acgcgcccaa cgtatcggca acgtgcggga acaaaaacgt
    59701 cccctcttcg gcaacctcgc catagtagcg atcgaacagt ttacgcagaa tacgtacctg
    59761 ttcttctgcc ggaatgtcgt catcaacggg cggtttcccc attgttttac gcagagtcgc
    59821 acgttcctga cgcgcccagg tcaatgcgcg ctccatcaga acatctgcgc cgttaccaat
    59881 ccaggtaata acgcgttctt cacctgcgac gggcaactcc agcgcataca gcgccatatc
    59941 taccgcagca gcaagaccag gagcactgtc gaccagcgta ccatcaagat caaaagcgac
    60001 gccgcgaata tcttcaaact tattcatgac ttacctttgc cagttcactg cgcatttcat
    60061 caatgacttt tttatagtct ggctggtcga agattgccga accggcgacg aacatatccg
    60121 cgcccgccgc agcgatttcg ccaatgttgt tcaccttcac gccaccgtcc acttccagac
    60181 gaatgtcaaa gccagactcg tcgatacggc gacgtacttc gcgcagttta tccagtgttt
    60241 gaggaatgaa agactgaccg ccgaaaccag ggttgacgga catcagcagg atcacatcca
    60301 gcttatccat cacgtaatcc agatagctca gaggtgtcgc cgggttaaat accagacccg
    60361 ctttacagcc attttcttta atcagttgca gcgtgcggtc aacatgctcg gaggcttctg
    60421 gatgaaaggt aatgatgctg gcaccagcgg cagcgaaatc aggcacaatg cgatcgacgg
    60481 gtttcaccat cagatgtacg tcgatagggg cggtaatgcc gtagttacgc aaggatttca
    60541 gcaccattgg cccaatcgtc agattgggaa catagtggtt atccatgacg tcaaaatgca
    60601 cgacatcagc gccagctgcc agggcttttg cggtatcttc acccaggcgg gcaaaatcag
    60661 ccgacagaat tgagggggca atcaaatact gtttcatccg cttctccttg agaattattt
    60721 tttcgcgggt gaaacgactc ctggtttgta caaagccagc agttcgtcca cctttttacg
    60781 tgtgccgccg ttgctgctta tactgcgtcg aactttgacg acatgcaatt ttgcgcgctg
    60841 ataccactca cgcgttaaca tcgtatcgtg attggagatc agcactggaa tatggcgctc
    60901 aaccagacct tcggcgatct ccgccagatg cgcttgttgt tcaagcgtaa aactgtttgt
    60961 gtgatacgcc gtaaagttgg cggtcgcaga cagcggtgca taaggcggat cgcaatagac
    61021 gacggatgca tcatctgcgc gctccatgct atcggcgtaa gactcacaat agaaaaaggc
    61081 attctgcgct ttttcagcga agtgatacaa ctctgcttcc gggaaatagg gttttttgta
    61141 gcggccaaac ggcacgttaa actcaccgcg cagattgtaa cgacacaggc cgttgtaacc
    61201 gtagcggttc aaatataaaa acagtaccgc ccgacggaac ggatcctggc ttttgttgaa
    61261 ctcttcgcgg aactgatagt aaacctcggc gcaatttgtt tcgggaacaa acagctcgcg
    61321 tgcggcctgt acgtactcat cagtacgcat cttcacaatg ttatagagac tgatcaggtc
    61381 gctattgata tcggcaagga tataacgaga aaagtcggtg ttgagaaaca ccgacccggc
    61441 acctacaaaa ggctcaacca gacattcgcc cttgggcaaa tgccgtttaa tatcatcaag
    61501 caggggatac ttgccccctg cccacttcaa aaaagcgcga tttttcttca tgccgattaa
    61561 ctaattacac cttctccggc tgtggagaaa gctccgacag catccagcgc ttcaggcagc
    61621 tcccgcaacg ataaaccatt gcgggagata accttgatta cttcagatcg gcctgtacct
    61681 gacgcagcgg tttcgcccac gggtttttgg cctgaacatc tgctggcaat gtagataccg
    61741 cttttttcgc ctcttctttc gaagcgtaca cgccagaaac caggacatac cacggctgac
    61801 cattacgcgt cgtttcatag acaacgtagt ttttcagatt ctctttcttc gcccaaccgt
    61861 tcaggttgtc gtagttagag gaactgctca gctgcagagt gtaatggctg gacggtgccg
    61921 atttcaacga accaacatta cctgcggtct tccctccagc ggctggtgtt gccgtggttt
    61981 gcgccgggga tgccgtctgt actggagccg tagtcgccgt ctcttttggt gctggcgtag
    62041 aagtcgcagc cggtgccttc gtgctcgcca caggggcagc tggttcagta cgcttcggcg
    62101 tctgtgctac cggcttcggc tccgttttca cggttgcttg cggttttttc ggttcaatca
    62161 ccgcctgctg acgaactggg cgcgtagtgg acggacgttc agcggtttgc gttttcgccg
    62221 agtcacgcga tgcattgcca ttgcgaacag gcgcaaccgt tgcgggttca gtcggcaacg
    62281 tggaattgac cgccacattg ttcagctgtt gctgattttg tggctgggtc agcgcattgt
    62341 tcaggtcacc ctgcacttca acacgttgtt gaccatccgt tgccaccggg gtttgccctt
    62401 gagtcggcgt agaagagatc ggtggcagcg aaacatcctg ctgagtattt tccgcagacg
    62461 tggttcccgg cgctggctgc acgccattcg cctgatcggt cgcattgcca gcaagatcaa
    62521 tactcttctc gccagacgcg gtttgatcgc tggaagtggt cgagggggct tttagcgcag
    62581 aaccgatacc gatgatcaac agcagtagaa ccagaatgcc gacgcccatc atcatatact
    62641 gacgagaagc gggtttactg gctgcttttt tgcgcttacg cggacgacgc tctacgcgct
    62701 cttcatccac ggtttcatct tcggattcgt caatttcttc ttcgatttcc ggttcctcat
    62761 tgcgctcttt ttgcgcacga gtcggacggc gatcgtcagt gtcatcaagt tcaatatcat
    62821 caaaattgat ctgcggttcg ccacgttcag tacgctcaga acgttcagaa gattgacgag
    62881 aacgaccagt acgacgatcg ctgggatcgg gtttcagctc gtcttctggt ttgaattcat
    62941 ccatttaaca ccccactaaa aggttaatgc ttaccacgtt gcaattaacc tgaagctaat
    63001 agaccgcttg ataagcggcc tgacctttct tgttgttacg ctgattgaca atcggcaatg
    63061 gcgttaagaa caagctcgtg cgaaacgccg ctgcgaactt cactcttacc aattgccaac
    63121 ggaagaatta agcgcatctc tcccgcaagg actttcttgt cacgcagcat atgcggtaaa
    63181 tacgcctgcg cggacatttc gcgcggccca ttgaccggta acccagcccg cgtgagcagg
    63241 gttataatac gctgcgtttc ggcagaacta aactgcccga gacgttccga cgtccgcgcc
    63301 gccatcacca tacccgcagc gaccgcttca ccatgtaacc aattgccata ccccatttca
    63361 gcttcaatgg catgaccaaa ggtgtgtccc agattcagta aagcacgtaa cccggtttcg
    63421 cgctcgtcgg cggcgacaac ttctgccttc agttcacaac aacggcgaat acagtacgcc
    63481 attgccggac cgtccagacg caacaacgca tccagattct cttccagcca gttaaaaaac
    63541 gcaccgtcaa gaataatgcc gtatttgatg acttctgcca gccccgacgc taactcacgc
    63601 gggggaagcg ttttcagaca gtcgagatcc accaccactg aagcaggctg gtagaacgcg
    63661 ccaatcatgt ttttaccgag gggatggttg accgcagttt tgccgccaac ggaggaatcg
    63721 acctgcgaca gtaacgtcgt cgggacttga atgaaacgaa caccgcgctg ataactcgcc
    63781 gccgcgaagc cggtcagatc gcccactacg ccgccgccaa gcgccaccag cgtagtatcg
    63841 cgaccatgcg gcttttgtaa caacgccgta aagacggtat cgagtacagc taggctttta
    63901 tactgctcgc cgtcagggag gataacgcta tcgacgttaa cacccgcctg ttcaagtacg
    63961 ccgcggacct tatcgagata cagaggagcc agggtttcgc tggtgaccaa catgacctgc
    64021 tcgcccgatt tcagcggtaa gaatgaagct ggttcattaa acaaaccaga tgcgatggta
    64081 attgggtaac tacgttcccc gagagtaacg acaatcctct ccataacgcg acatccacct
    64141 taattactgt acccgcagac gagtgtatat aaagccagaa ttagttgctt tccagcatgt
    64201 gaataatctg gtttgcaacc actttagcgc tttgatcatc agtacgaatg gtcacgtcgg
    64261 caatctcttc atacagcgga ttgcgttcat tggccaacgc ttccagaact tcacgcggcg
    64321 gtgtttcaac gtgcagcaac gggcgttttt tatcacgctg cgtgcgtgca agttgctttt
    64381 cgatggtcgt ttcaagataa acgacaacgc cacgagcgga aagacggtta cgcgtttcac
    64441 gggatttcac agagccgccg ccagtagcca gcacaatacc ctgtttctcg gtcaactcat
    64501 tgatgacctt ttcttcgcga tcgcggaagc cttcttcgcc ttctaaatcg aaaacccagc
    64561 ccacatcagc tccggttcgt ttctcaatct cttgatcgga atcgtaaaat tccatattga
    64621 gctgttgagc taactggcgc ccaatagtgc tttttccggc acccataggc ccaaccagaa
    64681 agatattgcg tttctctgcc attttttcgg tactactaag actattcgtt aatgataaac
    64741 ccgcttcgct cagagagcgc cgcaggacat gaactgaaac ctcataagat attgcgagag
    64801 tcagactgaa aattatctca atactccagc gggtttggca actgaataaa tcacccatcc
    64861 ctctgcatat ctggtcgctg caagcgcgct gccttgctac caccgctctg gcgataaatc
    64921 accgggtaag attagcgtaa aaaagacagc aaaatgccgc ctgaatgata aatcatcatc
    64981 atggaacgca ggcagcgaag agcaccaact ctcaaatcgg tactccttgt atgctaaata
    65041 cctgcgcgcg tcaaatagat gaaacacgtt cagcataaaa acattaccgc ttttacggct
    65101 gtttactcac tggaaaccag tcgtggcgtg ataaacacca ctaactcgcg tcgttcatct
    65161 tcttttccgt catgacgaaa taattgcccg aaccagggaa tgtcgccaag caacggtacg
    65221 ctatcctgac ccgatttatt tttacgggta aaaatgccgc ccagcgccaa cgtttctccg
    65281 ctttttacct cgacctgcgt ttcgatctcc tgcttatcaa tcgccagcac ttcgccatcg
    65341 gcctgctgta gcacctgccc cggaacgttc tggctgatgt gtaatttcag ccggatgcga
    65401 cctttttgta acaccgtggg cgtgacctcc atccccagga cggcctcttt aaattccacc
    65461 gacgtcgcgc cactttcccc gctggaaacc tgatatggaa tttcgctctc ctgtttaatg
    65521 ctggcaggct gaagatgtga ggccagcaga cgcggactgg cgataatatc cagctgctgt
    65581 ttttgttcga gcgcggaaag ctcaagatcc agcaagcgtc cgttgatgcg cccaatgtta
    65641 aaaccgacat gcgttgtcgc cgtcgctacg gagaggtcgc taccaagcgt ggtgacttgc
    65701 ccaacgccac cagcgtgttg cgcatcggcc agcgtccatt tcacgcctaa ctcacgcaaa
    65761 cttttttcat taatggtgac aatatgcgcc gacagctcaa cctgcccgac cggcagatcc
    65821 atttgcgcta cccactgttc aagcgcgctt aacgccgttt tgttatctcg tagcaaaagg
    65881 cgattggtgc gtttatcgac ggtcatactc cctttggcac tcagtagctt ctcccccgct
    65941 ttcgccagtt ctcccgcgtc ggcgtattgc agggttatac tgcgattttc cagcggcaga
    66001 tttgcctgcg cccgcgcctg ctccgcctcc tggcgggcga tattgtcatt ctgccaggca
    66061 atggaatgca ctgagagaat gttgccttcc tgccgcgtta tcagtccggc gcttttcact
    66121 acagtttgta gtgcctgctt ccagggaaca tctgttagat gtaacgacac cgtaccgctg
    66181 acgtctggcg acacgaccag gttcaacttc tcctgttcag ccagcgcctg caacacctga
    66241 gctaccggaa cgtcatccac catcagcgtc actttttgcg gctttgccgc ctgtacgccg
    66301 ggtatcagca tcaacagtag tgcggctatc cattgcttca tttgtatctc cttgccgttg
    66361 ccacaaccat tgtggcggtt cgcagtttgt cccggtaccc agcgttagta cgtctggcgt
    66421 cagctgtaaa attgtccagc cgttttccag cacatcgttt tgctgcaccc gtcgccattt
    66481 cttttgcccg tcttttatta caccgatgat gcgctcgcct cgccctacca tcccctgata
    66541 gcgccactgg ctaagttcgc taatccggca tagatcttcc ggcgatttaa aagggtcacg
    66601 cataccggtt aaaaggcaca atgcaatacc tgccaacaac cagcgtttaa ccctcatgcg
    66661 gcatctccag ttgtagcgtg aacaaaagat catcaccttc cacgcttaac gaaaaacggc
    66721 tcacgctgac gttgcgctct gccagccgtg taaatgccga cggcactgct tcccacagcg
    66781 ttttcaacgc caactcgcct ccctgcgcgg atggatgcca ggaaaccagt tgcgcgccgg
    66841 ataactgaaa atccagtggc gaaaagggca gcgttttttc ctcgctgaag ggagtggtgt
    66901 ctaccaggcg atacagtgcg gcccactgct ggtgatgact tgcccgtagt cgaattaatg
    66961 cgtcgcgctc ttcatggtgt gtcgatgata gaaaaatgag cgtaactaac atcagcaacc
    67021 agaatgccca gcaaaactgg cggaggcggg gtgatgtggc gaaccaccag tcaaagaaca
    67081 tgttcatcgc taaccttcct tgttaactga tactcaaatt gccagcgtcc ctgcgcatcc
    67141 tgctgcgtgg ctccccgctg attgagatga aaagacgcat cctggcggag tgacgtttct
    67201 agtgcgttta acgcggtaat gcttgttgtc agccccttga tctccagcgt tccctgctgc
    67261 cagcttatcg ttgtcagcca ggcgtgctcg ggtaaaagcg ccgccagtgc ttccagcgca
    67321 gattgccagt cgcgggtaaa ttgtcgctgg cgctgccgtt gcgaacgctg ttcgcgtaac
    67381 tgctgttgct ccagcaaacg tggcttcgtt atctgtaagc tgcgggcgag ttgttgttcc
    67441 gcctgaagca aaactgcgtc tatgcgcgct tcggcgctgc ctgtcagacg cagtattagc
    67501 gttatcccga cggccagcag cagaggcgca acgaacatca gcaaccagaa acgcagaaaa
    67561 gcggtccggc gttgctgtcg ccagggcaaa aaattaattg gcgggttcat cagtacgcct
    67621 tcccaagcgc cagccccagc gcgatggcaa agtctccacc aggcggcggt agcggcggct
    67681 gacgaacaga aacggcctcc caggggtcaa atccgccttc gccacatatc gcgacgctgt
    67741 cgggatcaac ggataacgct gccgccagct ccttcgcgct agtcatcccc accgccagtt
    67801 tgcgccccca gctatagcgc gtcgcccaca gccactgttc gttatcacgc caggccagac
    67861 attgctgatg agaaggtaaa aaaggcagga atcgctgtaa tgcactggca tccggggtga
    67921 tcgcactaac atgaacacgc aacctttctg ccagcgtaag cagcgttgcc agctctttgc
    67981 tttgcgcggc agtcacgtta taagcggggc tgagtgagtc ttcgctgtaa tcgaagcgca
    68041 gggagtccgg atccatatcc agctcgcggg ccatcgtccc tgacagccag gccgtttgct
    68101 cccgctcacc aagggacatc gacgggcgcg gaaatgaccg ctgtaatgtg cgactggcgg
    68161 gaaacgccaa cataatgtga tgacgctgcg gcagttcgcg actccacggt aacaacgttt
    68221 tagccagctg ctgcgcatca acaatccgcc catctttgat aatgtcgtgc gccagcggca
    68281 accgccacca gcgttgcaaa aagcattctt ttgcgccccg cacgatcgca accgctgccg
    68341 cttcttgctg ttgtaaatgc aaaccaattt gccagatctt aaatgtcatt gtgatgatct
    68401 ccttatcacc cgtcactctg acgggtatat caatgcgtct ggcttgcctt tatactaccg
    68461 cgcgtttgtt tataaactgc ccaaatgaaa ctaaatggga aatttccagt gaagttcgta
    68521 aagtattttt tgatccttgc agtctgttgc attctgctgg gagcaggctc gatttatggc
    68581 ctataccgct acatcgagcc acaactgccg gatgtggtga cattaaaaga tgttcgcctg
    68641 caaattccga tgcagattta cagcgccgat ggcgagctga ttgctcaata cggtgagaaa
    68701 cgtcgtattc cggttacgtt ggatcaaatc ccaccggaga tggtgaaagc ctttatcgcg
    68761 acggaagaca gccgcttcta cgagcatcac ggagttgacc cggtggggat cttccgtgcg
    68821 gcaagtgtgg cgctgttctc cggccacgcg tcacaagggg cgagtaccat tacccagcag
    68881 ctggcgagaa atttcttcct cagtccagaa cgcacgatga tgcgtaagat taaggaagtc
    68941 ttcctcgcga ttcgcattga acagttgctg acgaaagacg aaatcctcga gctttatctg
    69001 aacaagattt accttggtta ccgcgcctat ggtgtcggtg ctgcggcaca agtctatttc
    69061 ggtaaaacgg tcgaccaact gacgctgaac gaaatggcgg tgatagccgg gttgccgaaa
    69121 gcgccttcca cctttaatcc gctctactca atggatcgtg ccgtcgcgcg gcgtaacgtc
    69181 gtactgtcgc ggatgctgga tgaagggtat atcacccagc aacagttcga tcagacacgc
    69241 actgaggcga ttaacgctaa ttatcacgcg ccggagattg ctttctctgc gccgtacctg
    69301 agcgaaatgg tgcgccagga gatgtataac cgttatggcg aaagtgccta tgaagacggt
    69361 tatcgcattt acaccaccat cacccgcaaa gtgcagcagg ccgcgcagca ggcggtacgt
    69421 aataacgtgc tggactacga catgcgccac ggctatcgcg gcccggcaaa tgtgctgtgg
    69481 aaagtgggcg agtcggcgtg ggataacaac aagattaccg atacgctgaa ggcgctgccg
    69541 acctatggtc cgctgctgcc tgccgcagtc accagcgcca atcctcaggg agcgacggcg
    69601 atgctggcgg acgggtcgac cgtcgcattg agtatggaag gcgttcgctg ggcgcgtcct
    69661 taccgttcgg atactcagca gggaccgacg ccgcgtaaag tgaccgatgt tctgcaaacg
    69721 ggtcagcaaa tctgggttcg tcaggttggc gatgcatggt ggctggcaca agtgccggaa
    69781 gtgaactcgg cgctggtgtc gatcaatccg caaaatggtg ccgttatggc gctggtcggt
    69841 ggctttgatt tcaatcagag caagtttaac cgcgccaccc aggcactgcg tcaggtgggt
    69901 tccaacatca aaccgttcct ctacaccgcg gcgatggata aaggtctgac gctggcaagt
    69961 atgttgaacg atgtgccaat ttctcgctgg gatgcaggtg ccggttctga ctggcagccg
    70021 aagaactcac caccgcagta tgctggtcca attcgcttac gtcaggggct gggtcagtcg
    70081 aaaaacgtgg tgatggtacg tgcaatgcgg gcgatgggcg tcgactacgc tgcagaatat
    70141 ctgcaacgct tcggcttccc ggcgcaaaac attgtccaca ccgaatcgct ggcgctgggt
    70201 tcagcgtcct tcaccccaat gcaggtggcg cgcggctacg cggtcatggc gaacggcggc
    70261 ttcctagtgg acccgtggtt tatcagcaaa attgaaaacg atcagggcgg cgtgattttc
    70321 gaagcgaaac cgaaagtagc ctgcccggaa tgcgatattc cagtgattta cggtgatacg
    70381 cagaaatcga acgtgctgga aaataacgat gttgaagatg tcgctatctc ccgcgagcag
    70441 cagaatgttt ctgtaccaat gccgcagctg gagcaggcaa atcaggcgtt agtggcgaag
    70501 actggcgcgc aggagtacgc accgcacgtc atcaacactc cgctggcatt cctgattaag
    70561 agtgctttga acaccaatat ctttggtgaa ccaggctggc agggtaccgg ctggcgtgca
    70621 ggtcgtgatt tgcagcgtcg cgatatcggc gggaaaaccg gtaccactaa cagttcgaaa
    70681 gatgcgtggt tctcgggtta cggtccgggc gttgtgacct cggtctggat tggctttgat
    70741 gatcaccgtc gtaatctcgg tcatacaacg gcttccggag cgattaaaga tcagatctca
    70801 ggttacgaag gcggtgccaa gagtgcccag cctgcatggg acgcttatat gaaagccgtt
    70861 cttgaaggtg tgccggagca gccgctgacg ccgcctccgg gtattgtgac ggtgaatatc
    70921 gatcgcagca ccgggcagtt agctaatggt ggcaacagcc gcgaagagta tttcatcgaa
    70981 ggtacgcagc cgacacaaca ggcggtgcac gaggtgggaa cgaccattat cgataatggt
    71041 gaggcacagg aattgttctg attaaaaagg cgcttcggcg ccttttcagt tggctgacaa
    71101 agtgcatttg tttatgccgg atgcgggtga acgcgttatc cggctaacaa aatcgtgaaa
    71161 aatcaatata ttgcaggaac cccataagcc tgataagcgt tgtgcatcag gcaaacttca
    71221 cgcatttaca ctcgcccctg ccctttcaac cattcgcgca cgaggaacag cgcgctgaca
    71281 ttacgcgctt cgttgaagtc agggtcttcc agcaaatcca tcatatgcgc cagcggccag
    71341 cgcacctgtg gtagcggctc tggctcatcg ccttccagcg attccgggta gagatcttgc
    71401 gcgaccacga tattcatttt gctggaaaag taagacggtg ccatgctgag cttcttcaaa
    71461 aaagtcagat cgttcgcacc aaatccaacc tcttctttta gctcgcggtt agcggcttcg
    71521 tagacgcttt cacccggatc aattaatcct ttcgaaaaac ctaattcgta ggattcagtt
    71581 cccactgcgt attcgcggat caggatcagg tgatcatcca caatcggcac aatcatcact
    71641 gcttcccggt tggttggacg cattcgttca taaacacgcc gcacgccatt gctgaactcc
    71701 agatccacgc tctcgacggt aaacagtcgg gaacgggcta cagtttcaac attcagaatg
    71761 gtgggttttt gtaatgattt gttcatcgtg ggatctatgc tgtgaaatca gcggttattg
    71821 tgcgatatcg gacacgcttt cggcaatgtg aattgcatgt tatttacatt tatgtaactt
    71881 aataaataat cgtcctcaaa tcaaattaaa agtcaatagg ttgaaataac tccaggaatt
    71941 tgctgatatt ccgccttcag agggtttgct atgatcagcg gttactgtga tgtgcttaat
    72001 gatgctcaag ttaagctcca cgcttgccga tagccaaccg cagaatcatg tattgtgtcc
    72061 ggtgcgactg accacgcctg acagactaag taagatgggg aaagcatgag caccattgtg
    72121 atttttttag ctgctttgct ggcctgctca ctacttgcgg gatggctgat aaaagtgcga
    72181 tccagacggc gtcagctgcc ctggaccaac gccttcgcgg atgcgcaaac gcgtaaactc
    72241 acacctgaag aacgtagcgc cgttgaaaat tatcttgaga gcctgacgca ggtattacag
    72301 gtgcctggcc caacgggagc tagcgcggca ccgatctctc tggcgctgaa tgccgaaagc
    72361 aacaacgtca tgatgctgac acacgctatc acacgttacg gcatctctac cgacgatccg
    72421 aataaatggc gttactacct cgattcggta gaagtccacc tgcccccctt ctgggaacag
    72481 tacatcaacg atgagaatac cgttgaactg attcataccg attcgctgcc gttggttatt
    72541 tcactcaacg gtcatacgct gcaggagtac atgcaggaaa cgcgcggcta tgccttgcaa
    72601 cctgttccgt ccacgcaggc gtcgattcgc ggggaagaga gtgagcaaat cgagctactc
    72661 aatattcgca aagaaacgca tgaagaatat gcgctgagtc gtccgcgcgg gctgcgtgaa
    72721 gcgttgctga tcgtcgcctc cttcctgatg ttctttttct gcctgattac cccggatgta
    72781 tttgttccgt ggctggcagg cggcgcatta ctgctgctgg gcgcaggtct gtgggggcta
    72841 ttcgcgcccc cggcaaaatc ctccctgcga gaaattcatt gtctgcgcgg tacaccccgt
    72901 cgttggggat tgtttggcga aaacgatcag gaacagatca acaatatttc actcggtatt
    72961 atcgacctgg tctatcccgc acactggcag ccatacattg ctcaggatct cggtcaacaa
    73021 accgatattg atatctatct cgaccgccac gtagtgcgtc aggggcgata tctttcgctg
    73081 catgatgaag taaaaaactt tccgttacag cactggctgc gcagtacgat tatcgctgcg
    73141 ggctcgctgc tggtgctgtt tatgctgtta ttctggatcc cgctggatat gccgctgaaa
    73201 ttcactctct catggatgaa aggcgcgcag accattgaag ccaccagcgt aaaacaactg
    73261 gctgacgctg gcgtgcgggt aggcgataca ttgcgtatta gcggtacggg aatgtgtaat
    73321 attcgcactt ccggaacctg gagcgcgaaa accaattcac cttttttacc gtttgactgc
    73381 tcgcagatca tctggaatga cgcccgctca ttaccattac cagaatctga actggtcaac
    73441 aaagcgacgg cattgactga agcagttaat cgccagctgc accctaaacc ggaagatgaa
    73501 tctcgcgtca gtgcctcatt gcgttcagcg attcaaaaat ccggcatggt attgcttgat
    73561 gattttggcg acattgtact gaagacagcg gatttatgtt ctgccaaaga tgactgtgtg
    73621 cgactgaaaa atgcgctggt caatctcggc aacagtaaag actgggacgc gctggtaaaa
    73681 cgcgccaacg ccgggaagct cgatggcgtg aatgtgttat tacgcccggt gagtgcggaa
    73741 tcgctggata acctggtggc aacctccacc gcgccgttta tcacgcatga aacggcgcga
    73801 gcggcacaat cactaaacag tccggccccc ggcggattcc tgattgtcag cgacgaaggc
    73861 agcgattttg ttgatcagcc ctggccttcg gcatcacttt acgactaccc gccgcaagaa
    73921 cagtggaacg ctttccagaa actggcacaa atgctgatgc atacgccgtt taacgccgaa
    73981 ggtatcgtca caaaaatctt cactgacgcc aatggtacgc agcatattgg ccttcatccg
    74041 atcccggatc gttccggcct gtggcgctat ctcagcacca cattgctgct actgacgatg
    74101 ctgggtagcg ccatttacaa tggcgtacag gcctggcgtc gttaccagcg tcatcgcact
    74161 cgcatgatgg agattcaggc ctattatgaa agctgcctga acccgcaact gatcacccct
    74221 tcagaaagcc ttatcgaata acacgtttgc gcggcaggtt atgctaccct gtcgcgcaaa
    74281 ttgcttcact ctggagattt ccctcatgca tatcaacatt gcctggcagg acgtagatac
    74341 cgttctgctg gatatggacg gcacgttgct cgacctcgcc ttcgataact atttctggca
    74401 aaagctggtg cctgaaacat ggggcgcgaa aaacggggtt acgccacagg aagcgatgga
    74461 atatatgcgc cagcaatatc acgacgtaca gcatacgcta aactggtact gtcttgatta
    74521 ctggagtgag caactgggtc tggatatctg tgcgatgacc accgagatgg gaccgcgcgc
    74581 cgtactgcgt gaagatacca ttccgtttct tgaggcactg aaagccagcg gtaagcagcg
    74641 aattttgctc accaatgctc atccgcacaa cctggcggta aaacttgagc atacgggtct
    74701 ggacgcacac cttgatttat tactttccac ccacacattt ggttatccga aagaggatca
    74761 gcggttatgg catgcggtgg ccgaagctac gggtctgaaa gctgaaagaa cgctgtttat
    74821 tgatgacagc gaagcgattc tcgatgctgc cgcgcaattt ggtattcgtt actgcctcgg
    74881 cgtgactaat cctgattccg ggattgccga gaaacagtat caacgccatc cgtcactgaa
    74941 tgactaccgc cgcctgatcc cctcgctaat gtgaaggaga cgccatgaaa gagaaacctg
    75001 ctgttgaggt tcgactggat aaatggctat gggctgcccg tttttataaa acccgcgcgc
    75061 tggcccgtga aatgattgaa ggcggtaagg tgcattacaa cgggcagcgc agcaagccga
    75121 gcaaaatcgt cgagctgaat gccacgctca ctctgcgcca gggaaatgac gaacgcacgg
    75181 tgattgtaaa ggcgattact gaacagcgtc gccccgccag cgaggcagcc ttgctgtatg
    75241 aagagactgc ggaaagtgta gagaaacgcg aaaaaatggc gatggcacgt aaacttaatg
    75301 ccttaaccat gccgcacccg gaccgacgcc cggacaaaaa agagcgccgc gacctgttac
    75361 gatttaaaca cggcgacagt gaataactgt cacctgcaag agagatgatt atgccgcaac
    75421 atgaccaatt acatcgctat ctgtttgaaa attttgccgt tcgcggcgaa ctggtaaccg
    75481 tttcggaaac cctgcaacag atccttgaga accacgatta tccgcagccc gttaaaaacg
    75541 tgctggcaga actgctggtt gcgaccagcc tgttaaccgc tacgctgaag tttgatggtg
    75601 atatcaccgt acagctgcag ggcgacggtc cgatgaatct ggcggttatt aacggtaaca
    75661 ataaccagca aatgcgcggt gtggcgcgcg tgcagggcga aattccagaa aatgccgacc
    75721 tgaaaacgct ggtcggcaat ggttacgtgg tgatcaccat taccccgagc gaaggcgaac
    75781 gctatcaggg cgtagttggt ctggaaggtg ataccctggc ggcctgccta gaagattact
    75841 ttatgcgttc tgaacagctg ccgacgcgcc tgtttattcg caccggcgac gtagacggca
    75901 aaccggctgc aggcggtatg ttgttgcagg taatgcctgc gcaaaatacc caacaggacg
    75961 actttgacca cctggcgacg ctaaccgaaa ccatcaaaac cgaagaactg ctgaccttac
    76021 cggcaaacga agtgttgtgg cgtttgtatc acgaagaaga ggtgacggtt tacgatccgc
    76081 aggatgtgga gttcaaatgc acctgctcgc gtgaacgttg cgccgatgcg ctgaaaacgc
    76141 tgcctgatga agaagttgat agcatcctgg cggaagatgg cgaaattgac atgcattgtg
    76201 attactgcgg taaccactat ctgttcaatg cgatggatat tgctgaaatc cgcaacaacg
    76261 cgtctccggc agatccgtaa gttcattaat gagtatgtcc ggcagagagg gctctgccgg
    76321 attttacgac ttaatggcct gatgtgctgc gcttaccagc gcctatgaaa taaatcgcaa
    76381 attattttgg tcagatacag cgtcgtatcc gataaaagcg ttgtcactta cttaagttta
    76441 tttccagcgt ttcaccatct accgtcagat cgcgccacgc tttcggcctc gtccccagtt
    76501 ttccatcctt cgcagctgtg agtaattttt ctttcgttat ttcgttcgga agtaaactca
    76561 tatcaagcgc gtcccattct tttctgtctg agtcaaagcc atagacaatg actctgtcat
    76621 cattaaaagc aaacaggatc cgctccgcct gaccatcact attcagatct tgctcgacca
    76681 gtacgcaggc gtctttttca atacaggtca tcacgttata gcgatcctgg attaaggccg
    76741 accagaatgt cgcatccggt ttaacagaac caggggcaat taacacattt tcggcgaata
    76801 ctttttccga tacctgttcc tgaagatgtt gctctccatc taacgccatc agcagatccc
    76861 gcgcgcgttt cgggtctttc ataaacccgg catcgctttt cagcgactca agcgccgcac
    76921 gtccatagcg accgctctgt tcgagcatgt agatagttac ctggtctggc gtgtttttgc
    76981 cgctctgata acgcgccata tgactgttca cgctaatacg catactgtcc agcaccggcg
    77041 aattaagaag caccagtatc accaacacca ataacgaaac cgcaaggttc actttgccct
    77101 gaagaacaag gggattttgc ccattacgcc agacgatact gacaaaatac cccagcgacc
    77161 acaccagtaa caccagctcc gccagcgcgc cctgcaagcg gtcgacagtc cagccgtact
    77221 gagcgacccg cagccataac ccccaggtgg cgacgaacac atacagcgga gcaaccagca
    77281 aagcggtttt aatcaggcaa cgcaacggcc ccgtccaggg aagtgacgct ttttgcggat
    77341 cgcggacaat agccattaag atcaattgca aaaaggccag cgttaacagc aacccggcgg
    77401 cggagatgtg gcgagaaatc gcgctcaggc ccgtaaacgg cagggtgatg ataaacatcg
    77461 gggttagcaa tgatactaac ggcagcaacc ccgtggcgat tagcgtgaac aacttttgaa
    77521 tagagtcgat taaacgtgac tgtgtacgcg cgaggatcac cgccagtgcg gtaaccagac
    77581 caaatgtaag atacataaac cagtcggttg caaaaaagag cgtattgaaa aacgtgatgc
    77641 caacaagttt aaacaactca ccccacagta aaagcaccag ccacgtcagg ccattggaga
    77701 gaaaaatcac cagtaatata agtacgttat gccatactga ctggtaaaag tagctatagc
    77761 ggctactgcc attgcggata cgaaggcttt gttgtatcca cggtagcaac aacattgcca
    77821 tcaacagcag atagcagcca aaatcccaaa tcgccttttc agctctccag gggttcatgc
    77881 catcagtttg ccacttcagc caaccgctca tccctgtcgt ggcaataaac accagcgcca
    77941 gccatcccca aaggcgcttc tgtttaaaag agatcacgga gaagagtaaa acggatgaaa
    78001 acgcgacggt cgcaggcacg ccataaacaa tccaactgtg attttttccg gcaagccagg
    78061 cgataagcag gtagcaaacc agcccctgaa gtaatccggt agcaatcatc ccccagcgtg
    78121 ttgccggtga aagttcaacg ttatccatgt tggttatcca gaatcaaaag gtgggttaat
    78181 tatcgcatcc gggcagtagt attttgcttt tttcagaaaa taatcaaaaa aagttagcgt
    78241 ggtgaatcga tactttaccg gttgaatttg catcaatttc attcaggaat gcgattccac
    78301 tcacaatatt cccgccatat aaaccaagat ttaacctttt gagaacattt tccacaccta
    78361 aaatgctatt tctgcgataa tagcaaccgt ttcgtgacag gaatcacgga gttttttgtc
    78421 aaatatgaat ttctccagat acgtaaatct atgagccttg tcgcggttaa cacccccaaa
    78481 aagactttac tattcaggca atacatattg gctaaggagc agtgaaatgc gcgttaacaa
    78541 tggtttgccc ccgcaagaac tcgaggctta tggtatcagt gacgtacatg atatcgttta
    78601 caacccaagc tacgacctgc tgtatcagga agagctcgat ccgagcctga caggttatga
    78661 gcgcggggtg ttaactaatc tgggtgccgt tgccgtcgat accgggatct tcaccggtcg
    78721 ttcaccaaaa gataagtata tcgtccgtga cgataccact cgcgatactt tctggtgggc
    78781 agacaaaggc aaaggtaaga acgacaacaa acctctctct ccggaaacct ggcagcatct
    78841 gaaaggcctg gtgaccaggc agctttccgg caaacgtctg ttcgttgtcg acgctttctg
    78901 tggtgcgaac ccggatactc gtctttccgt ccgtttcatc accgaagtgg cctggcaggc
    78961 gcattttgtc aaaaacatgt ttattcgccc gagcgatgaa gagctggcag gtttcaaacc
    79021 agactttatc gttatgaacg gcgcgaagtg cactaacccg cagtggaaag aacagggtct
    79081 gaactccgaa aacttcgtgg cgtttaacct gaccgagcgc atgcagctga ttggcggcac
    79141 ctggtacggc ggcgaaatga agaaagggat gttctcgatg atgaactacc tgctgccgct
    79201 gaaaggtatc gcttctatgc actgctccgc caacgttggc gagaaaggcg atgttgcggt
    79261 gttcttcggc ctttccggca ccggtaaaac caccctttcc accgacccga aacgccgcct
    79321 gattggcgat gacgaacacg gctgggacga tgacggcgtg tttaatttcg aaggcggctg
    79381 ctacgcaaaa actatcaagc tgtcgaaaga agcggaacct gaaatctaca acgctatccg
    79441 tcgtgatgcg ctgctggaaa acgtcaccgt gcgtgaagat ggcactatcg actttgatga
    79501 tggttcaaaa accgagaaca cccgcgtttc ttatccaatc tatcacatcg ataacatcgt
    79561 taagccggtt tccaaagcgg gccacgcgac taaggttatc ttcctgactg ctgatgcttt
    79621 cggcgtgttg ccgccggttt ctcgcctgac tgccgatcaa acccagtatc acttcctctc
    79681 tggcttcacc gccaaactgg ccggtactga gcgtggcatc accgaaccga cgccaacctt
    79741 ctccgcttgc ttcggcgcgg cgttcctgtc gctgcacccg actcagtacg cagaagtgct
    79801 ggtgaaacgt atgcaggcgg cgggcgcgca ggcttatctg gttaacactg gctggaacgg
    79861 cactggcaaa cgtatctcga ttaaagatac ccgcgccatt atcgacgcca tcctcaacgg
    79921 ttcgctggat aatgcagaaa ccttcactct gccgatgttt aacctggcga tcccaaccga
    79981 actgccgggc gtagacacga agattctcga tccgcgtaac acctacgctt ctccggaaca
    80041 gtggcaggaa aaagccgaaa ccctggcgaa actgtttatc gacaacttcg ataaatacac
    80101 cgacacccct gcgggtgccg cgctggtagc ggctggtccg aaactgtaat gatttgaagc
    80161 tggagaatat ctatccagta tcttatagaa agcaaaacgg gaggcacctt cgcctcccgt
    80221 ttatttaccc ttcttttgtc gtgccctgcg cccgcgttac cggcactggc agccaggcgc
    80281 gaatggaaag cccgccccgc tcgctggtgc caagctccag catcccgtta tggttatcca
    80341 cgatacgctg cacaattgcc agccctaatc ccgtgccgct aatggtgcgc gcactgtcgc
    80401 cgcggacaaa cggctggaac aggtgcttac gttgttccgg cgcaattccc ggaccgtcat
    80461 cttccacctg gaaccaggcg cgattcggct ccgttccgct gctgactttg atccagccat
    80521 tgccataacg ggcggcgttg accaccatat tcgccaccgc gcgtttgatc gacagcgggt
    80581 gcattttcac ttcaatgctg ccggggtaaa gcgcggtttc aatttcccgc tcatagccac
    80641 tttcggcagc aatcacctca ccgagcactg cattaagatc cgccatttcc atcggcatct
    80701 cctgcccggt gcgcaggtag tcgataaact gctcaatgat ggcgttgcac tcttcgatat
    80761 ctttattgat cgattctgcc agatagccat cctgctcgct catcatctca gtcgccaggc
    80821 gaatacgcgt cagcggcgtg cgcaagtcgt gacttacccc cgccatcagc agcgtgcggt
    80881 catccgccag ttgcttaaca ccagccgcca tatggttaaa ggcacgggta acggaacgca
    80941 cctccgaagc gccatattca cgcagcggcg gcggaataat ccctttacca acctgcaagg
    81001 ctgcgtgttc gagatcgacc aacggtcggt tctggatacg aataaacagc cacgccccgc
    81061 ctatcgccaa tagcataatc gccagcgtat agcggaacag cggagagaaa tcgccctgat
    81121 gaatttcggt cagcggcacg cgtacccaga tattgggcga cagccaggtt ttcagccaga
    81181 cgacaggcga acttttgttg acctcaacgc gcacttccgt cgggccgccc agttgctgtg
    81241 ccatctgatg gcttaagaat tcatagtgtt gcgcccaacg cagacctgcc tcttcggcag
    81301 cctcgttgga gtagagagag atccccagct cacggtagat ctcccgacgg aaagcgggag
    81361 gcacaaccaa ctgcgtgccg tcctccagtt gcagtttgtc ggtcatcaac atacgcactt
    81421 cgtacgcgag gactttatta aactgctgga ggctcggcaa aatcgcgaag ttcagcacca
    81481 ccagataagt cgtcaccagg ctggcgaaca gcaaggtgac gatgagcaat aacgtacggg
    81541 caaatgaact tcgtggcgag aagcgcaatc gcctcatgct ttagagccgt ccggtacaaa
    81601 gacgtagccc aggccccaaa cggtctgaat gtaacgcgga tgcgctggat cttcttctac
    81661 catgcggcgc agacgcgaaa tctgcacgtc gatggagcgt tccattgcgg aatattcacg
    81721 accacgggca aggttcatca gcttatcgcg ggagagcggc tcacgcggat ggctgaccag
    81781 tgccttcagt accgcaaact caccgctggt gagcggcatc ggctcatctt cgcggaacat
    81841 ttcgcgcgta ccgaggttaa gtttgaactt accgaaagca attaccgcct cttcctgtga
    81901 cggtgcgcct ggcagttcgt tcgcctgacg acgcagcacc gcacgaatac gggccagcag
    81961 ttcacgcggg ttaaacggtt ttggaatgta gtcgtcagcg ccaatctcca ggcctacgat
    82021 acggtccact tcttcccctt tcgccgtcac cataatgatc ggcatcgggt tgctctgact
    82081 acgaagacgt cggcaaatcg acaagccatc ttcaccaggt aacattaaat ccagtaccat
    82141 aagatggaaa gattcacgag tcagcaggcg atccatctgt tctgcattag cgacgcttcg
    82201 aacctggaag ccttgttcgg tgagataacg ttccagcagc gcacgcaggc gcatgtcgtc
    82261 atcgaccacc agaatcttgt agttctcttg cattgtttgt actcccaaag gttcgcaaca
    82321 atttgtaagc gtgtattctt aaaaaagctc acgttcgtca ccagctaaat ctggtataaa
    82381 tttcagccta aattgttaca aagcatatta aacagcagct taagtataca atttattcgg
    82441 cgaaacatta ttgattctgt tgatatgatc acgttatacc caatgtgcgc attatcaaac
    82501 agacaaaggg aatcaacgag atgaaaacgc ccctggttac ccgggaaggg tatgaaaaac
    82561 tcaaacaaga gcttaattat ctctggcgtg aagaacgccc ggaggtcaca aaaaaggtga
    82621 cctgggccgc aagtctgggc gaccgcagcg aaaatgctga ctatcagtat aataaaaagc
    82681 gtctgcgtga aatcgaccgt cgcgtgcgct atctcactaa atgcctggaa aatctcaaaa
    82741 tcgtcgatta ctcccctcag caggaaggca aagtattttt tggcgcgtgg gtggagattg
    82801 aaaacgacga tggcgtgact caccgtttcc gtattgtcgg ctacgatgaa atttttggcc
    82861 gtaaagatta catctctatc gattccccga tggcccgcgc attgctgaaa aaagaagtcg
    82921 gcgatctggc ggtggtgaat acccctgccg gggaagcgaa ctggtatgtt aatgctatcg
    82981 agtacgtgaa accgtaagga agagtcttaa cctcctgccg atggctggca tttttgccag
    83041 ccagtccgta taactatccc ctgatttttg atccgaaaag atgaactcaa accatgatga
    83101 atgattcgtt ctgccgcatt attgcgggtg aaattcaggc gcgcccggaa caggttgacg
    83161 ctgccgttcg cctgcttgac gaagggaata ccgtgccgtt tatcgcacgt tatcgtaagg
    83221 aaatcaccgg cggtctggat gacacgcagc tgcgtaatct ggaaacgcgt ctgagctatc
    83281 tgcgcgagct ggaagagaga cgtcaggcga tcctcaaatc catttccgag caaggcaaac
    83341 tcaccgatga tctggcgaag gccatcaacg ccaccctaag caaaaccgaa ctcgaagacc
    83401 tctacctgcc ctacaaacct aaacgccgca cccgcgggca aatcgccatt gaagcagggc
    83461 ttgagccgtt ggctgacctg ctgtggagcg atccgtcaca cacgccagaa gtcgccgctg
    83521 cacaatatgt tgatgccgat aaaggcgtgg cagataccaa agccgcgctg gatggcgcgc
    83581 gctatatcct gatggaacgg tttgccgaag atgccgcgct gctggcgaaa gtgcgtgatt
    83641 atctgtggaa gaacgcgcat ttggtttcta cggtggtgag cggtaaagaa gaggaagggg
    83701 cgaaattccg cgactatttc gatcatcacg aaccgttgtc cacggtgcct tctcaccgcg
    83761 cgctggcgat gttccgtggg cgtaacgaag gcgtactcca gctttcgctg aatgccgatc
    83821 cgcagttcga tgagccgccc aaagagagct attgcgagca aatcatcatg gatcaccttg
    83881 gcctgcgcct gaacaatgcc ccggcggata gctggcgcaa aagcgtagtg agctggacct
    83941 ggcgcatcaa ggtgctgatg catctggaaa ccgaactgat gggtaccgtg cgcgaacgcg
    84001 cggaagatga agcaatcaac gtctttgccc gtaacctgca cgatctgctg atggcggccc
    84061 ctgccggact gcgtgcaacg atgggcctcg atccgggtct gcgtaccggg gtaaaagtgg
    84121 cggtggtcga tgccactggc aaactggtgg cgaccgatac catttacccg cacaccggac
    84181 aagccgcaaa agcagcgatg accgttgctg ccttgtgtga aaaacataac gttgaactgg
    84241 tggcgatcgg caacggtaca gcttcccgcg aaactgaacg tttctatctc gacgtgcaga
    84301 agcagttccc gaaagtgacc gcacagaaag tgatcgtcag cgaagctggc gcgtcggttt
    84361 actcagcttc cgagctggca gcgcaggagt tcccggatct cgacgtttcg ctgcgtggtg
    84421 cggtatctat cgcccgccgt ttgcaggatc cgctggcgga gctggtgaaa atcgatccga
    84481 aatctatcgg cgtaggtcag tatcagcatg acgtcagcca gacgcaactg gcccgcaaac
    84541 tggatgcagt agtagaagac tgcgtaaacg ccgttggcgt cgatctcaac accgcttctg
    84601 ttccgctatt aacccgcgtg gcgggcctga cgcgcatgat ggcgcaaaac atcgtggcct
    84661 ggcgcgatga gaacggccag ttccagaacc gtcagcaact gctgaaagtc agccgtctgg
    84721 ggccgaaagc cttcgagcag tgcgcgggct tcctgcgcat taaccacggt gataacccgc
    84781 tggacgcctc taccgttcat ccggaagcct atccggtggt ggaacgcatt ctggcagcaa
    84841 cacagcaggc actgaaagat ctgatgggta acagcagcga actgcgtaac ctgaaagcgt
    84901 ctgactttac tgatgaaaaa ttcggtgtgc cgacagtaac tgacatcatc aaagagctgg
    84961 aaaaaccggg tcgcgatccg cgtccggaat ttaaaaccgc tcagtttgcc gatggcgtcg
    85021 agacaatgaa cgacctgcaa ccgggtatga tcctcgaagg cgcagtgacc aacgtcacca
    85081 actttggcgc gtttgtcgat attggcgtgc atcaggacgg cctggttcac atctcttcat
    85141 tatcgaacaa gtttgtggaa gatccgcata ccgtggtgaa agcgggcgac attgtgaagg
    85201 tgaaagtgct ggaagtggat cttcagcgta aacgtatcgc cctgactatg cgtctggacg
    85261 agcagcctgg cgaaaccaac tctcgtcgcg gcggcggtaa tgaacgcccg caaaacaacc
    85321 gcccggcagc caaaccacgc ggtcgtgaag cgcagcctgc cggtaatagc gcgatgatgg
    85381 atgcgctggc ggcagcgatg ggtaaaaaac gttaaacgcc cggactggcc cctaaacctg
    85441 cgcgataagg cgttcacgcc gcatctggca acgaacgctt tgcctgacat aaaagtgccg
    85501 gagaatatct ccggcacttt tattccacag ccaaactcac aatatactcc gttaatattt
    85561 atcacttcat taacaactga aaccttaatt aaacattagc cagtccgggt aactcactat
    85621 tcgaattata ttttcgctgc gatataacct tgagccacat caacattgag tcagattatt
    85681 attcaaacca acattcgcac acattttaag tattgctgat agaaaccatt ctcattatca
    85741 ttgtgttgtt gattatttaa tctctccttt gttggcaaat catctggtct catgtcgcgg
    85801 tcaaacgccc catgaggtag ttatccagtt aatgagaaac aagtaggcac ctatgcaata
    85861 cactccagat actgcgtgga aaatcactgg cttttcccgt gaaatcagcc cggcatatcg
    85921 ccaaaaactg ctttctctag gcatgttacc tggctcctct tttgatgtgg tgcgcgtcgc
    85981 tccactcggc gaccccattc atatcgaaac ccgtcgtgtg agcctggtat tacgcaaaaa
    86041 agatctggcc ttattagaag tggaagcggt ttcctgttaa tacggtgata acaacaatga
    86101 aaaaattaac cattggctta attggtaatc caaattctgg caagacaacc ttatttaacc
    86161 agctcactgg cgcacgtcag cgtgtaggta actgggctgg cgttaccgtc gaacgtaaag
    86221 aagggcaatt ctccaccacc gatcatcagg tcacgctggt ggacctgccc ggcacctatt
    86281 ctctgaccac catctcatcg cagacctcgc tcgatgagca aatcgcctgt cactacattt
    86341 tgagtggcga cgccgacctg ctgattaacg tggtggatgc gtctaacctt gagcgtaacc
    86401 tgtacctgac gctgcaactg ctggaactcg gcattccctg catcgtggcg ctgaacatgc
    86461 tcgacattgc cgagaagcaa aatattcgta ttgaaattga tgctctatcg gcacgtcttg
    86521 gctgcccggt gatcccgctg gtttcaaccc gtggtcgcgg tattgaagcg ctcaagctgg
    86581 cgattgatcg ctataaagct aacgagaatg tggaactggt gcattacgca cagccgctac
    86641 tcaacgaagc agattcactg gcaaaagtga tgccttccga catcccgctg aaacaacgtc
    86701 gctggctggg cctgcaaatg ctggaaggcg atatctacag ccgcgcctac gccggtgaag
    86761 cgtcgcagca tctggatgcc accctcgccc gtctgcgtaa tgaaatggat gatccggcgc
    86821 tgcacattgc cgatgcgcgt taccagtgca ttgctgccat ctgtgatgtg gtaagcaaca
    86881 ccctgacggc agaacccagc cgtttcacca ctgcggtaga taaaatcgtg ctcaaccgtt
    86941 tcctcggtct gccgattttc ctctttgtga tgtacctgat gttcctgctg gctatcaaca
    87001 tcggcggggc gttacagccg ctgtttaacg tcggctccgt ggcgctattt gtgcatggta
    87061 ttcaatggat tggctacacg ctccacttcc cggactggct gactatcttc ctcgcccagg
    87121 gcctgggtgg cggtattaac accgtgctgc cactggtgcc gcagatcggc atgatgtacc
    87181 tgttcctctc cttccttgag gactccggct atatggcgcg tgcagcgttt gtgatggacc
    87241 gcctgatgca ggcgctgggc ttgccgggga aatcctttgt gccgctgatc gtcggtttcg
    87301 gttgtaacgt accgtcggta atgggcgcac gtacgcttga tgcaccgcgt gaacgtctga
    87361 tgaccatcat gatggcaccg tttatgtcct gcggcgcgcg tctggctatc ttcgcagtat
    87421 tcgcggctgc cttcttcggg cagaacggtg ccctggcggt tttctcgctg tatatgctcg
    87481 gtatcgtgat ggcggtgctg actggcctga tgctcaagta caccatcatg cgcggtgaag
    87541 cgacgccgtt tgtcatggag ctgccggtct accatgtacc acacgttaaa agcctgatta
    87601 tccagacctg gcagcgtctg aagggcttcg ttctgcgtgc tggtaaagtg attatcatcg
    87661 tcagcatttt cctgagcgct ttcaacagct tctcgttgag cgggaaaatc gtcgataaca
    87721 tcaacgactc ggcgctggct tccgtcagcc gggtgataac ccctgtcttc aagccgattg
    87781 gcgtacatga agataactgg caggcaacgg ttggcctgtt tacgggcgca atggcaaaag
    87841 aagtggtggt gggtacgctc aacaccctct acaccgcaga aaatattcag gacgaagagt
    87901 tcaatccggc ggaattcaac ctcggtgaag agctgttcag tgcggtagat gaaacctggc
    87961 agagcctgaa agacaccttc agccttagcg tactgatgaa ccccattgaa gctagcaaag
    88021 gcaacggtga aatgggtacc ggggcgatgg gcgtgatgga tcagaaattc ggtagcgcag
    88081 ctgccgcata cagctacctg attttcgtcc tgctgtatgt accatgtatc tcggtgatgg
    88141 gagccatcgc ccgtgaatca agccgtggct ggatgggctt ctccatcctg tgggggctga
    88201 atatcgctta ctcactggca acattgttct atcaggtcgc cagctacagt cagcacccaa
    88261 cttacagcct ggtgtgcatt ctggcggtaa tcctgtttaa catcgtagct atcggtctgc
    88321 tgcgccgcgc gcgtagccgg gtggatatcg aactgctggc aacccgcaag tcggtaagca
    88381 gttgctgcgc agccagcacc accggtgatt gccattaatg gcttcactta ttcaggtacg
    88441 tgatttgctg gcgttacggg gccgtatgga agcgacccag ataagccaga cattgaacac
    88501 tccgcagcca atgattaacg ccatgctgca acagctggaa agcatgggca aagccgtgcg
    88561 gattcaggaa gaacctgacg gctgcctctc tggcagttgt aaaagctgcc cggaaggcaa
    88621 agcctgtctg cgcgagtggt gggcgctgcg ttaaccatac tccatcgcct gttttgaaaa
    88681 gcccggtatg cgtttgcatc cgggcttttt tgcgtgcggc tttccataaa aatgcaattc
    88741 ttgcagcgca acgtaagttc ctctgaaagc gtctcgcagg gatgaaaact cgctaatgca
    88801 cagatgtgga gtggcgcgta gagtcgcggc attcaaacaa caggtgaagg aacgccatga
    88861 gtaaaaagca gagttccacc ccacatgatg cgctgttcaa actcttttta cgccaaccgg
    88921 agacggcacg cgattttctt gcgtttcatt taccggcacc cattcacgcg ctctgtgata
    88981 tgaaaaccct caagctggag tcgaggagct ttattgatga cgatctgcgt gaaagctatt
    89041 ccgatgtgct gtggtcggtg aaaacggaac aaggaccagg atacatctat tgtctgattg
    89101 aacatcaaag cacctcaaac aaactgatcg catttcgcat gatgcgttac gctattgccg
    89161 caatgcaaaa tcaccttgat gctggataca aaacgttgcc gatggtggtg ccattgttgt
    89221 tttaccacgg tattgaaagc ccctatccct attcgctgtg ttggctggat tgtttcgccg
    89281 atcccaaact ggcaaggcag ctttatgcct ccgcatttcc gctgattgat gtcaccgtca
    89341 tgcctgatga tgaaatcatg cagcaccgac gtatggcgct gctggagtta attcaaaaac
    89401 atattcgtca acgcgatctg atggggctgg tagagcaaat ggcctgctta ttaagtagtg
    89461 gatacgctaa tgacagacaa atcaaagggc tgtttaatta catactgcaa actggcgacg
    89521 ctgtacgttt taacgatttt atcgacggcg ttgccgaacg ttcaccgaaa cacaaggaga
    89581 gtttaatgac tattgcggaa agattgcggc aggaggggga acaatccaaa gccctgcata
    89641 tagccaaaat aatgcttgaa tccggagtcc ctcttgcaga catcatgcga tttaccgggc
    89701 tgtcagaaga agagttggct gcggcgagta gactggcccc ctgaatctcc agacaaccag
    89761 tat
//
LOCUS       DAEEUE010000002        84294 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.002, whole genome shotgun sequence.
ACCESSION   DAEEUE010000002 DAEEUE010000000
VERSION     DAEEUE010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 84294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84294
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.002"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            311..1744
                     /gene="cscA"
                     /locus_tag="KLK84_000106"
     CDS             311..1744
                     /gene="cscA"
                     /locus_tag="KLK84_000106"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBE2007607.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1752..2810)
                     /locus_tag="KLK84_000107"
     CDS             complement(1752..2810)
                     /locus_tag="KLK84_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBE2007608.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2978..3196
                     /locus_tag="KLK84_000108"
                     /pseudo
     CDS             2978..3196
                     /locus_tag="KLK84_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3214..4542
                     /gene="dsdA"
                     /locus_tag="KLK84_000109"
     CDS             3214..4542
                     /gene="dsdA"
                     /locus_tag="KLK84_000109"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HBE2007609.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4650..6188)
                     /gene="emrY"
                     /locus_tag="KLK84_000110"
     CDS             complement(4650..6188)
                     /gene="emrY"
                     /locus_tag="KLK84_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HBE2007610.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6188..7351)
                     /gene="emrK"
                     /locus_tag="KLK84_000111"
     CDS             complement(6188..7351)
                     /gene="emrK"
                     /locus_tag="KLK84_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HBE2007611.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7767..8381
                     /gene="evgA"
                     /locus_tag="KLK84_000112"
     CDS             7767..8381
                     /gene="evgA"
                     /locus_tag="KLK84_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HBE2007612.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8386..11979
                     /gene="evgS"
                     /locus_tag="KLK84_000113"
     CDS             8386..11979
                     /gene="evgS"
                     /locus_tag="KLK84_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HBE2007613.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(12035..13180)
                     /gene="yfdE"
                     /locus_tag="KLK84_000114"
     CDS             complement(12035..13180)
                     /gene="yfdE"
                     /locus_tag="KLK84_000114"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HBE2007614.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13254..14198)
                     /gene="yfdV"
                     /locus_tag="KLK84_000115"
     CDS             complement(13254..14198)
                     /gene="yfdV"
                     /locus_tag="KLK84_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HBE2007615.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14268..15962)
                     /gene="oxc"
                     /locus_tag="KLK84_000116"
     CDS             complement(14268..15962)
                     /gene="oxc"
                     /locus_tag="KLK84_000116"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HBE2007616.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(16016..17266)
                     /gene="frc"
                     /locus_tag="KLK84_000117"
     CDS             complement(16016..17266)
                     /gene="frc"
                     /locus_tag="KLK84_000117"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HBE2007617.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17779..18402)
                     /locus_tag="KLK84_000118"
     CDS             complement(17779..18402)
                     /locus_tag="KLK84_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HBE2007618.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18699..18974
                     /locus_tag="KLK84_000119"
     CDS             18699..18974
                     /locus_tag="KLK84_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2007619.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(19051..19293)
                     /locus_tag="KLK84_000120"
     CDS             complement(19051..19293)
                     /locus_tag="KLK84_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HBE2007620.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19646..20566
                     /gene="lpxP"
                     /locus_tag="KLK84_000121"
     CDS             19646..20566
                     /gene="lpxP"
                     /locus_tag="KLK84_000121"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HBE2007621.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20921..20992
                     /gene="ypdK"
                     /locus_tag="KLK84_000122"
     CDS             20921..20992
                     /gene="ypdK"
                     /locus_tag="KLK84_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HBE2007622.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(21057..22295)
                     /gene="alaC"
                     /locus_tag="KLK84_000123"
     CDS             complement(21057..22295)
                     /gene="alaC"
                     /locus_tag="KLK84_000123"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HBE2007623.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22672..24158
                     /locus_tag="KLK84_000124"
                     /pseudo
     CDS             22672..24158
                     /locus_tag="KLK84_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            24173..24907
                     /gene="ypdB"
                     /locus_tag="KLK84_000125"
     CDS             24173..24907
                     /gene="ypdB"
                     /locus_tag="KLK84_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HBE2007624.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24920..25777
                     /locus_tag="KLK84_000126"
     CDS             24920..25777
                     /locus_tag="KLK84_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE2007625.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQTQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFSLTP
                     GEYSARFQG"
     gene            complement(25780..28011)
                     /gene="ptsP"
                     /locus_tag="KLK84_000127"
     CDS             complement(25780..28011)
                     /gene="ptsP"
                     /locus_tag="KLK84_000127"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBE2007626.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIMLTLGANEGMNHVKVFSQLARKLVNKNFRQSLFAAQDAQSILTLLETE
                     LTF"
     gene            complement(28036..29073)
                     /gene="ypdE"
                     /locus_tag="KLK84_000128"
     CDS             complement(28036..29073)
                     /gene="ypdE"
                     /locus_tag="KLK84_000128"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBE2007627.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVIGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29073..30156)
                     /gene="ypdF"
                     /locus_tag="KLK84_000129"
                     /pseudo
     CDS             complement(29073..30156)
                     /gene="ypdF"
                     /locus_tag="KLK84_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30171..31418)
                     /locus_tag="KLK84_000130"
     CDS             complement(30171..31418)
                     /locus_tag="KLK84_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBE2007628.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31440..31766)
                     /locus_tag="KLK84_000131"
     CDS             complement(31440..31766)
                     /locus_tag="KLK84_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HBE2007629.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(31985..32950)
                     /gene="glk"
                     /locus_tag="KLK84_000132"
     CDS             complement(31985..32950)
                     /gene="glk"
                     /locus_tag="KLK84_000132"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HBE2007630.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33154..34410
                     /locus_tag="KLK84_000133"
     CDS             33154..34410
                     /locus_tag="KLK84_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HBE2007631.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34525..34851
                     /locus_tag="KLK84_000134"
     CDS             34525..34851
                     /locus_tag="KLK84_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBE2007632.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(34992..36230)
                     /locus_tag="KLK84_000135"
     CDS             complement(34992..36230)
                     /locus_tag="KLK84_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBE2007633.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36566..37768
                     /gene="nupC"
                     /locus_tag="KLK84_000136"
     CDS             36566..37768
                     /gene="nupC"
                     /locus_tag="KLK84_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HBE2007634.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(37818..39930)
                     /locus_tag="KLK84_000137"
                     /pseudo
     CDS             complement(37818..39930)
                     /locus_tag="KLK84_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40138..40213)
                     /locus_tag="KLK84_000138"
     tRNA            complement(40138..40213)
                     /locus_tag="KLK84_000138"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40178..40180),aa:Ala,seq:ggc)
     gene            complement(40253..40328)
                     /locus_tag="KLK84_000139"
     tRNA            complement(40253..40328)
                     /locus_tag="KLK84_000139"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40293..40295),aa:Ala,seq:ggc)
     gene            40549..40908
                     /locus_tag="KLK84_000140"
     CDS             40549..40908
                     /locus_tag="KLK84_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBE2007635.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            40910..41302
                     /locus_tag="KLK84_000141"
     CDS             40910..41302
                     /locus_tag="KLK84_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBE2007636.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41354..42769)
                     /gene="gltX"
                     /locus_tag="KLK84_000142"
     CDS             complement(41354..42769)
                     /gene="gltX"
                     /locus_tag="KLK84_000142"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBE2007637.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43028..43103
                     /locus_tag="KLK84_000143"
     tRNA            43028..43103
                     /locus_tag="KLK84_000143"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43061..43063,aa:Val,seq:tac)
     gene            43148..43223
                     /locus_tag="KLK84_000144"
     tRNA            43148..43223
                     /locus_tag="KLK84_000144"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43181..43183,aa:Val,seq:tac)
     gene            43228..43303
                     /locus_tag="KLK84_000145"
     tRNA            43228..43303
                     /locus_tag="KLK84_000145"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43261..43263,aa:Lys,seq:ttt)
     gene            43334..43459
                     /locus_tag="KLK84_000146"
     ncRNA           43334..43459
                     /ncRNA_class="other"
                     /locus_tag="KLK84_000146"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            43735..44076
                     /locus_tag="KLK84_000147"
     CDS             43735..44076
                     /locus_tag="KLK84_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HBE2007638.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(44067..44993)
                     /locus_tag="KLK84_000148"
     CDS             complement(44067..44993)
                     /locus_tag="KLK84_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE2007639.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            45083..46081
                     /locus_tag="KLK84_000149"
     CDS             45083..46081
                     /locus_tag="KLK84_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HBE2007640.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(46078..46296)
                     /locus_tag="KLK84_000150"
     CDS             complement(46078..46296)
                     /locus_tag="KLK84_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HBE2007641.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46298..48313)
                     /gene="ligA"
                     /locus_tag="KLK84_000151"
     CDS             complement(46298..48313)
                     /gene="ligA"
                     /locus_tag="KLK84_000151"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBE2007642.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48384..49370)
                     /gene="zipA"
                     /locus_tag="KLK84_000152"
     CDS             complement(48384..49370)
                     /gene="zipA"
                     /locus_tag="KLK84_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HBE2007643.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            49600..50361
                     /gene="cysZ"
                     /locus_tag="KLK84_000153"
     CDS             49600..50361
                     /gene="cysZ"
                     /locus_tag="KLK84_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HBE2007644.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50546..51517
                     /gene="cysK"
                     /locus_tag="KLK84_000154"
     CDS             50546..51517
                     /gene="cysK"
                     /locus_tag="KLK84_000154"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBE2007645.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            51901..52158
                     /gene="ptsH"
                     /locus_tag="KLK84_000155"
     CDS             51901..52158
                     /gene="ptsH"
                     /locus_tag="KLK84_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HBE2007646.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52203..53930
                     /gene="ptsI"
                     /locus_tag="KLK84_000156"
     CDS             52203..53930
                     /gene="ptsI"
                     /locus_tag="KLK84_000156"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HBE2007647.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            53971..54480
                     /gene="crr"
                     /locus_tag="KLK84_000157"
     CDS             53971..54480
                     /gene="crr"
                     /locus_tag="KLK84_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBE2007648.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(54522..55373)
                     /gene="pdxK"
                     /locus_tag="KLK84_000158"
                     /gene_synonym="thiJ"
     CDS             complement(54522..55373)
                     /gene="pdxK"
                     /locus_tag="KLK84_000158"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HBE2007649.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            55478..55852
                     /locus_tag="KLK84_000159"
     CDS             55478..55852
                     /locus_tag="KLK84_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HBE2007650.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            55885..56608
                     /locus_tag="KLK84_000160"
                     /pseudo
     CDS             55885..56608
                     /locus_tag="KLK84_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(56812..57723)
                     /gene="cysM"
                     /locus_tag="KLK84_000161"
     CDS             complement(56812..57723)
                     /gene="cysM"
                     /locus_tag="KLK84_000161"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HBE2007651.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(57857..58954)
                     /gene="cysA"
                     /locus_tag="KLK84_000162"
     CDS             complement(57857..58954)
                     /gene="cysA"
                     /locus_tag="KLK84_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HBE2007652.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(58944..59819)
                     /gene="cysW"
                     /locus_tag="KLK84_000163"
     CDS             complement(58944..59819)
                     /gene="cysW"
                     /locus_tag="KLK84_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HBE2007653.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(59819..60652)
                     /gene="cysT"
                     /locus_tag="KLK84_000164"
     CDS             complement(59819..60652)
                     /gene="cysT"
                     /locus_tag="KLK84_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HBE2007654.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDS
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(60652..61668)
                     /gene="cysP"
                     /locus_tag="KLK84_000165"
     CDS             complement(60652..61668)
                     /gene="cysP"
                     /locus_tag="KLK84_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HBE2007655.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(61972..62763)
                     /gene="ucpA"
                     /locus_tag="KLK84_000166"
     CDS             complement(61972..62763)
                     /gene="ucpA"
                     /locus_tag="KLK84_000166"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HBE2007656.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(62950..63750)
                     /gene="murR"
                     /locus_tag="KLK84_000167"
     CDS             complement(62950..63750)
                     /gene="murR"
                     /locus_tag="KLK84_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HBE2007657.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            63914..64810
                     /gene="murQ"
                     /locus_tag="KLK84_000168"
     CDS             63914..64810
                     /gene="murQ"
                     /locus_tag="KLK84_000168"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBE2007658.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            64814..66238
                     /gene="murP"
                     /locus_tag="KLK84_000169"
     CDS             64814..66238
                     /gene="murP"
                     /locus_tag="KLK84_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HBE2007659.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(66418..67317)
                     /gene="yfeX"
                     /locus_tag="KLK84_000170"
     CDS             complement(66418..67317)
                     /gene="yfeX"
                     /locus_tag="KLK84_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HBE2007660.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(67413..67988)
                     /locus_tag="KLK84_000171"
     CDS             complement(67413..67988)
                     /locus_tag="KLK84_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HBE2007661.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(68049..68498)
                     /locus_tag="KLK84_000172"
     CDS             complement(68049..68498)
                     /locus_tag="KLK84_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HBE2007662.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(68485..68910)
                     /locus_tag="KLK84_000173"
     CDS             complement(68485..68910)
                     /locus_tag="KLK84_000173"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HBE2007663.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            69124..69993
                     /gene="amiA"
                     /locus_tag="KLK84_000174"
     CDS             69124..69993
                     /gene="amiA"
                     /locus_tag="KLK84_000174"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HBE2007664.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            69997..70896
                     /gene="hemF"
                     /locus_tag="KLK84_000175"
     CDS             69997..70896
                     /gene="hemF"
                     /locus_tag="KLK84_000175"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HBE2007665.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(70902..71954)
                     /gene="eutR"
                     /locus_tag="KLK84_000176"
                     /pseudo
     CDS             complement(70902..71954)
                     /gene="eutR"
                     /locus_tag="KLK84_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(72000..72500)
                     /gene="eutK"
                     /locus_tag="KLK84_000177"
     CDS             complement(72000..72500)
                     /gene="eutK"
                     /locus_tag="KLK84_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HBE2007666.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(72513..73172)
                     /gene="eutL"
                     /locus_tag="KLK84_000178"
     CDS             complement(72513..73172)
                     /gene="eutL"
                     /locus_tag="KLK84_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBE2007667.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            74354..75145
                     /locus_tag="KLK84_000179"
     CDS             74354..75145
                     /locus_tag="KLK84_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2007668.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            75865..76260
                     /locus_tag="KLK84_000180"
     CDS             75865..76260
                     /locus_tag="KLK84_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBE2007669.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            76278..76541
                     /locus_tag="KLK84_000181"
     CDS             76278..76541
                     /locus_tag="KLK84_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2007670.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            76564..77106
                     /locus_tag="KLK84_000182"
     CDS             76564..77106
                     /locus_tag="KLK84_000182"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2007671.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMHKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            77245..77532
                     /locus_tag="KLK84_000183"
     CDS             77245..77532
                     /locus_tag="KLK84_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2007672.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            77571..77777
                     /locus_tag="KLK84_000184"
     CDS             77571..77777
                     /locus_tag="KLK84_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2007673.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            78089..78574
                     /locus_tag="KLK84_000185"
     CDS             78089..78574
                     /locus_tag="KLK84_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBE2007674.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            78555..78833
                     /locus_tag="KLK84_000186"
     CDS             78555..78833
                     /locus_tag="KLK84_000186"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2007675.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            78826..80286
                     /locus_tag="KLK84_000187"
     CDS             78826..80286
                     /locus_tag="KLK84_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBE2007676.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            80320..81129
                     /gene="murI"
                     /locus_tag="KLK84_000188"
     CDS             80320..81129
                     /gene="murI"
                     /locus_tag="KLK84_000188"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBE2007677.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(81248..81942)
                     /locus_tag="KLK84_000189"
     CDS             complement(join(81248..81696,81696..81942))
                     /locus_tag="KLK84_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE2007678.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(82461..83834)
                     /gene="eutA"
                     /locus_tag="KLK84_000190"
     CDS             complement(82461..83834)
                     /gene="eutA"
                     /locus_tag="KLK84_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBE2007679.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(83809..84288)
                     /gene="eutP"
                     /locus_tag="KLK84_000191"
     CDS             complement(83809..84288)
                     /gene="eutP"
                     /locus_tag="KLK84_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HBE2007680.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
BASE COUNT        21902 a        21329 c        20003 g        21060 t
ORIGIN      
        1 aatccggaaa caagtgagcg tttccggatt cttacacagc cactggatcg gtcaactgat
       61 ccttaactga tcggcattaa tactttggct gacatttttt tcccttaaat tcatctgagt
      121 tacgcatagt gataaacctc tttctcgcaa aatcgtcatg gatttactaa aacatgcata
      181 ttcgatcaca aaacgccata gttaacgtta acatttgcga tattcatcgc atttatgaaa
      241 gtaagggact atatttttat aaaagttaac gttaataatt caccaaattt gcttaaccag
      301 gatgattaaa atgacgcaat ctcgattgca tgcggcgcaa aacgcactag caaaacttca
      361 tgagcaccgg ggtaacactt tctatcccca ttttcacctc gcgcctcctg ccggatggat
      421 gaacgatcca aacggcctga tctggtttaa cgatcgttat cacgcgtttt atcaacatca
      481 cccgatgagc gaacactggg gaccaatgca ctggggacat gccaccagtg acgatatgat
      541 ccactggcag catgagccta ttgcgctagc gccaggagac gataatgaca aagacgggtg
      601 tttttcaggt agtgctgtcg atgacaatgg tgtcctctca cttatctaca ccggacacgt
      661 ctggctcgat ggtgcaggta atgacgatgc aattcgcgaa gtacaatgtc tggctaccag
      721 tcgggatggt attcatttcg agaaacaggg tgtgatcctc actccaccag aaggaatcat
      781 gcacttccgc gatcctaaag tgtggcgtga agccgacaca tggtggatgg tagtcggggc
      841 gaaagatcca ggcaacacgg ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg
      901 ggccttcgat cgcgtactgg cccacgctga tgcgggtgaa agctatatgt gggaatgtcc
      961 ggactttttc agccttggcg atcagcatta tctgatgttt tccccgcagg gaatgaatgc
     1021 cgagggatac agttaccgaa atcgctttca aagtggcgta atacccggaa tgtggtcgcc
     1081 aggacgactt tttgcacaat ccgggcattt tactgaactt gataacgggc atgactttta
     1141 tgcaccacaa agctttttag cgaaggatgg tcggcgtatt gttatcggct ggatggatat
     1201 gtgggaatcg ccaatgccct caaaacgtga aggatgggca ggctgcatga cgctggcgcg
     1261 cgagctatca gagagcaatg gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt
     1321 acgccagcag catcaatctg tctctccccg cagaatcagc aataaatatg ttttgcagga
     1381 aaacgcgcaa gcagttgaga ttcagttgca gtgggcgctg aagaacagtg atgccgaaca
     1441 ttacggatta cagctcggca ctggaatgcg gctgtatatt gataaccaat ctgagcgact
     1501 tattttgtgg cggtattacc cacacgagaa tttagacggc taccgtagta ttcccctccc
     1561 gcagcgtgac acgctcgccc taaggatatt tatcgataca tcatccgtgg aagtatttat
     1621 taacgacggg gaagcggtga tgagtagtcg aatctatccg cagccagaag aacgggaact
     1681 gtcgctttat gcctcccacg gagtggctgt gctgcaacat ggagcactct ggctactggg
     1741 ttaacataat atcaggtgga acaacggatc aacagcgggc aagggatccg cgtcactctt
     1801 cccccttcac gaccttcaat aatatgcaat gcagcttccc gcccgataat gtcatgtgga
     1861 agctgaattg tggtcagcgg cggtaaaaac agatgcccga cgccaaccag attatcaaag
     1921 cccattacag cgacatcctg cgggatacgt acccccttcg ccaaaagaac ctgataagcc
     1981 acaaaggctg cgcgatcgtt accacatatc agaacatcaa aatctggttt gcccgatttg
     2041 aagtgagcat tgagtaaact tgcgagatcg gtgtagtgat catcacctgt tgccatgtga
     2101 aattgtttca cctcagccag atctcgtcca gcatcacgcc aagcctgctc aaatccctgc
     2161 cgacgatacc ctgttgccaa cgcactttcc ggtagccaga agcataatgg ttgacgatag
     2221 cccgccgcga gcaaatgctg tgttgattca tattgtgcag tgtaatcatc agggatataa
     2281 ctgggtaacg ctgggtcatc cgccacacag ttcgccaata caatattttc accatacaga
     2341 gactcaggca gcgtgatatg tcgcagcccc attgtagtat agataatgcc atccggacgg
     2401 tgggcaagca gctgacgtgc cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa
     2461 aaactattcc agccgaactc gctggcggtt tgctcaatgg caagcagaat atcaacagag
     2521 aaaggagtgg tagccgtgtc ctgcgccagc acggcgagag tcgacggctt acgtccttga
     2581 gcgcgcatct tacgggcgga aagatcagga acataattca gggtctggat tgcctgcaat
     2641 acgcggtcac gcgttgcagg acgcacagat tctgcattat gcatcacccg ggagactgtc
     2701 atcatcgaca ctcccgccag gcgtgcgaca tcctttaatg aagccatacc caagccgttt
     2761 gccgtaaaac gggcactgta gcagaaacag acgtcactgg cgagatccaa cgccctatca
     2821 cctgacacag caatacaata aaaaataaca ataattcccg gacaattgtc cccaattccg
     2881 cctctgttct cgccaacgag taaccatata ggcaagtaag taaaacatgc caactggcat
     2941 cgccaacctg gagaagaaaa tataaatatg gatcattgtg ctgccactgt atcccgacat
     3001 cagcccggaa attattgcga ttgctatcgg ttcaggtgca attggctgca cgatcgttac
     3061 ggactcgctt ttctggctgg tgaagcaata ttgcggcgct acgctcaatg aaacatttaa
     3121 atactatacg acagcgacat ttatcgcttc agtcgtcgct ctggcgggca cattcctgct
     3181 gtcatttatc atctaagcgc aaagagacgt actatggaaa acgctaaaat gaactcgctc
     3241 atcgcccagt atccgttggt aaaggatctg gttgctctta aagaaaccac ctggtttaat
     3301 cctagcacga cctcattggc tgaaggttta ccttatgttg gcctgaccga acaggatgtt
     3361 caggacgccc atgcgcgctt atcccgtttt gcaccctatc tggcaaaagc atttgctgaa
     3421 acagctgcca ctggggggat tattgaatca gaactggttg ccattccggc tatgcaaaaa
     3481 cggctggaaa aggaatatca acaaccgatc agcgggcaac tcctactgaa aaaagatagc
     3541 catttgccca tttccggctc tataaaagca cgcggcggga tttatgaagt cctggcacat
     3601 gcagaaaaac tggctctgga agcggggttg ctgacgcttg atgatgacta cagcaaactg
     3661 ctttctccgg agtttaaaca gttctttagc caatacagca ttgctgtggg ctcaaccgga
     3721 aatctggggt tatcaatcgg cattatgagc gcccgcattg gctttaaggt gacagttcat
     3781 atgtctgctg atgcccgggc atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc
     3841 gtggaatatg agcaagatta tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct
     3901 gacccgaact gtttctttat tgatgacgaa aattcccgca cgttgttcct tgggtattcc
     3961 gttgctggcc agcgtcttaa agcgcaattt gcccagcaag gccgtatcgt cgatgctgat
     4021 aaccctctgt ttgtctatct gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc
     4081 gggcttaaac tggcgtttgg cgatcatgtt cactgctttt ttgccgaacc aacgcactcc
     4141 ccttgtatgt tgttaggcgt ccatacagga ttacacgatc agatttctgt tcaggatatt
     4201 ggtatcgaca accttaccgc agcggatggc cttgcagttg gtcgcgcatc atgctttgtc
     4261 gggcgggcaa tggagcgtct gctggatggc ttctataccc ttagcgatca aaccatgtat
     4321 gacatgcttg gctggctggc gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg
     4381 ggtatggccg gacctcagcg cgtgtgtgca tcagtaagtt accaacagat gcacggtttc
     4441 agcgcagaac aattgcgtaa tgccactcat ctggtgtggg cgacgggagg tggaatggtg
     4501 ccggaagaag agatgaatca atatctggca aaaggccgtt aataacgttt caacgcaaca
     4561 tcgcaatcct ttccctgggt gagcgatgct gccgatggcg cagacttaag atccccggtc
     4621 ttacccgcta taaccccctc cttttatcat cacccaacgc ctttcgctgt aaacggcggt
     4681 ttcgcaaacc aaaccaaaac cgtaagtaag ataaaagcaa tagccgccat acggaaaatc
     4741 tcatttgcag aaatagaaag tgactgctgg gttatttcat tattaatttc attaagaact
     4801 cctgaaagcg aaccatagta tttatccata atttgtgacg atgaattaaa cacggggtta
     4861 aattgatcga tggttgctgt caactgacta tggtgtaacg attcgcgtcg tccccacagc
     4921 gtcattgtca acgacgtacc aactgatcct gacaaggtac gaaaaaaatt actcatactc
     4981 gaggcattgg caaatttatt atctggcaag cctgaaaacg aaatcgttgt taagggtaaa
     5041 aagaaacagg caacggcgaa tccctggaaa aactgtggca aaatgatgcc tgtaaaatca
     5101 atcgttggca taaatgtcac agaacgccag taatagcaaa ccgcatacat caaaaaactg
     5161 aatgtcacta acacccgcat gtctatttta ttgccataac gtcctatcaa aggtgaaatc
     5221 aatagtggca tgatgccgat gggcgcataa gcaagcccgg cccatatcgc attatacccc
     5281 atcgttttct ggagtaactg cggcataagg acgatcgctc cagagtaaaa taaatacgcg
     5341 caggtgatac tcacaatacc aatggtgaaa ttacgggact taaacaaact gagatcaaga
     5401 atcgggttct ctgaggtcga ttcccaaatg actaaagaga tcagagaaat aactgatact
     5461 actgttaata ttattattgt actcgagttg aaccaatcca gatcgcgccc tttatcaagc
     5521 ataatttgca agccaccaac accgagcact aacagggtca gtcttggtag attcattttt
     5581 accggtgaag tttcagtttc tcttccttta agtaaggtta agcataatgt caggacgata
     5641 atccccatag ggacattgat taaaaatatc caaccccagc taaagttatc gcaaatatag
     5701 ccgcccaata tcggcccaca tatcggagcg ataatcacgg tcattgacca taatgccaga
     5761 gcaaatgttc ttttttctgg tggataattc cttaataata aactctgtga cagtggaatt
     5821 aacggccccg ccattaatcc ctgaacgact ctaaaaaata tcagcacatc aagattggtc
     5881 gataaactac acattaagga agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt
     5941 aattcgccta ttctttgtgc cagcctgccg gtaacaggga tcgcaatggc atttgctacg
     6001 ccaaacgagg tgataaccca ggtgccttcg tctgttgatg ctcccagaaa gccagatatt
     6061 gtcggtattg cgacgttaga aatagtggaa tccaacattt gcataaacgt cgctaatgac
     6121 aatgcaatag taacgcacca taacgtccca ccggttaatg gtgccggagt tgatttagtg
     6181 attgccatta aagttgtcca ttatgcgaaa taatgttgct aatttctttt tctatcggac
     6241 tggtatcgat aactaaagcc ttactggtat aagcaggcat ggaggtcacg gttgaagcca
     6301 gatcaggcat ctcagcaatg tcttcgtcct tcgtatcaat agttgcagtc atcgataaac
     6361 caatgcgcaa ggggtgttcc atgagttctt ttggatcaag agaaacttca accggtacac
     6421 gctgaacgat tttgatccag ttccctgtcg cattttgtgc aggtaataag gagaacgcat
     6481 tgccggttcc catattgatc cctgtcaccc gaccatgaaa cacaacattt tcaccataaa
     6541 gatcgctgat aatattgact gattgaccaa tccgtacatc cgtgagttgt gtttctttaa
     6601 agttggcatt aacccacatt tgacgtgccg gtacgacagc cattaacgat tgtccggaac
     6661 tcactgtttc gccgacctga acacttcgct gggcaatata gccagtaacc ggactcctta
     6721 tatccgtacg tttaagcacc aaccaggctt ctttggttgc atccgccgct tcaactactt
     6781 gtggctgacg gtttaatggt gtgttcatta ctaaagcttt attcgcttta taagcctgga
     6841 tagcggcatt cagtgccgct ttgctactta ttaacgtatc tttggtatgc tccagcgttt
     6901 cttttgaaat aaccccctgc ttcgctaaag gcacacgacg gttataatct tctaaagatt
     6961 gttgatactg aatacgtgct gaagcgactt cggcactgta ttgtttatcc tgtaagtaca
     7021 gtttattcgt ttgccgaaca atatttgcca gattattttt agctttattg agtgcgatag
     7081 tggcatcagt tttatccagt gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt
     7141 tatgattaac tacagtgaca ctacctgaga cttgtgcaga aattggatct gcattccccg
     7201 tgacataggc gtcatctgta ctaatcatgt cttctaattc cattgaccaa taggcatagg
     7261 cacctgaaaa cgcaataaat aaaactatca ccaataaaga aaagtatttt tttctgttag
     7321 actgtttttt atttgaatta atctgttcca ctattatctc tcatttcgca tagatgaaat
     7381 ttatgaattg aatgaaaaga gtattcattc ttcatatcaa acacagtgca tgtgttgcac
     7441 attatcccag gaacgttgga ggcctaattt aaatgaggtg caaaaaaaac aacagaggac
     7501 taaaccgtgg cttttgcaat acaattctta cgcctgtagg attagtgaga agacttatag
     7561 tgctaacttg aaactataaa tcatcggtat aatccctgat tttattgttg acatttcatt
     7621 tatgccgact atttatatgg tatacttgtc gaattatctt aaaggaaact cagattttct
     7681 tatttttatt gagaaaatga gatgacgcct tatgtctgta ttactacagg gagaagggag
     7741 atgcttcatt gcaaagggaa taatctatga acgcaataat tattgatgac catcctcttg
     7801 ctatcgcagc aattcgtaat ttattgatca aaaacgatat tgaaatctta gcggagttaa
     7861 ctgaaggcgg aagcgccgtt cagcgggtgg aaacacttaa gcctgatata gtcatcattg
     7921 atgtcgatat ccccggagtt aacggtatcc aagtgttaga aacgctgagg aagcgccaat
     7981 atagcggaat tattattatc gtctccgcta aaaatgacca tttttacggg aaacattgtg
     8041 ctgatgctgg cgctaatggt ttcgtgagta aaaaagaagg catgaacaat atcattgcgg
     8101 ctattgaagc tgcaaaaaat ggctactgct atttcccctt ctctctcaac cggtttgttg
     8161 gaagtttaac gtccgaccag caaaaactcg actccttatc gaaacaagaa attagtgtca
     8221 tgcggtatat tcttgatggc aaggataata atgacattgc ggaaaaaatg ttcatcagca
     8281 acaaaactgt cagcacgtat aaaagtcgcc tgatggaaaa attagaatgt aaatcactga
     8341 tggatcttta cacattcgcg caacgtaaca aaatcggcta atcacatgaa gtttttaccc
     8401 tatttttttc ttctctgttg tggtctttgg tcgaccataa gtttcgcaga cgaagattac
     8461 atcgaatatc gtggcatcag tagtaacaac cgtgtcacac ttgatccagt acgtctgagc
     8521 aacaaggaat tacgttggtt agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc
     8581 caaacggcta ccttgttgca taccgattcg cagcaacagg ttcgtggtat taatgctgat
     8641 tatttaaatc ttttaaaaag agcgttaaat atcaaattaa cactccggga atacgcagat
     8701 catcaaaaag caatggacgc gcttgcagaa ggtgaagtcg atatagtgtt atcacattta
     8761 gttacttcgc cgcctcttaa taatgacatt gctgcaacca aaccattgat aattaccttt
     8821 ccggcgctgg taaccaccct tcacgactca atgcgaccgc ttacctcacc aaaaccagta
     8881 aatattgctc gggtagcaaa ttacccccca gacgaggtaa ttcatcaatc atttccaaaa
     8941 gcaacaatta tctcttttac aaatttatat caggcattag catccgtctc agctgggcac
     9001 aatgattact ttattggtag taacatcatt accagcagta tgatttcccg ctatttcact
     9061 cactccttaa atgtagtgaa atattataac tcgccgcgtc aatataattt tttcttgacc
     9121 agaaaagaat ctgtcattct taatgaagta ctcaatagat ttgttgatgc tttaacaaat
     9181 gaagttcgct atgaagtatc acaaaattgg cttgatacag gaaacctggc ctttctgaac
     9241 aaaccattag aactcactga acatgaaaaa cagtggatta agcagcatcc caatttaaag
     9301 gtgctggaaa atccttactc gcccccctat tctatgacgg atgaaaatgg ctcggttcgg
     9361 ggcgttatgg gggacattct taatattatt accttgcaaa caggtttaaa tttttctccg
     9421 atcaccgttt cacacaatat ccatgctgga acacaactta gccccggagg atgggatata
     9481 atacctggcg ctatttatag tgaagatcga gaaaataatg ttttatttgc tgaagccttc
     9541 ataacaacgc cttacgtttt tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa
     9601 aaaggaatga aagttgccat tccatattat tatgagctgc attcgcaatt aaaagagatg
     9661 tatccggagg ttgaatggat acaggtcgat aatgccagcg ctgcatttca caaggttaag
     9721 gaaggtgaac ttgatgctct ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat
     9781 tactatccta atgaacttta tcattttctt attcctggcg ttccgaatgc atcgctttcg
     9841 ttcgcttttc ctcgcggaga accggaactt aaggatatta ttaataaagc actgaatgca
     9901 attcccccaa gcgaagttct gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc
     9961 attgacacat gggacctata tagcgagcaa ttttatattg ttacgacatt atccgtttta
    10021 ttagttggca gtagcctttt atggggattc tacctgttac gctcagttcg tcgtcgtaaa
    10081 gtcattcagg gtgatttaga aaaccaaata tcattccgaa aagcactctc ggattcctta
    10141 ccgaatccaa cttatgttgt aaactggcaa ggtaatgtca ttagtcataa tagtgctttt
    10201 gaacattatt tcactgcgga ttactacaaa aatgcaatgt taccattaga aaacagtgat
    10261 tcacccttta aagatgtttt ttctaatgcg catgaagtca cagcagaaac gaaagaaaat
    10321 cgaacaatat acacacaggt atttgaaatt gataatggca tcgagaaaag atgcattaat
    10381 cactggcata cattatgcaa tcttcctgca agtgacaatg cagtatatat ttgtggttgg
    10441 caagatatta ctgaaacgcg tgatctaatt aatgcactcg aggtagaaaa aaataaagcg
    10501 ataaaggcta ccgtagcaaa aagtcagttt ctggcaacga tgagtcacga aataagaaca
    10561 ccaataagct ctattatggg cttcctggaa cttctgtcgg gttctggtct tagcaaggag
    10621 caacgggtgg aggcgatttc acttgcctac gccaccggac aatcactcct cggcttaatt
    10681 ggtgaaatcc ttgatgtcga caaaattgaa tcgggtaact atcaacttca accacaatgg
    10741 gtcgatatcc ctactttagt ccagaacact tgtcactctt tcggtgcgat tgctgcaagc
    10801 aaatcgatcg cattaagttg cagcagtaca tttcctgatc attacctggt taagatcgac
    10861 cctcaggcgt ttaagcaggt cttatcaaat ttactgagta atgctctcaa atttaccacc
    10921 gagggggcag taaaaattac gacctccctg ggtcacattg atgacaacca cgctgttatc
    10981 aaaatgacga ttatggattc tggaagtgga ttatcgcagg aagaacaaca acaactgttt
    11041 aagcgctaca gccaaacaag tgcaggtcgt cagcaaacag gttctggttt aggcttaatg
    11101 atctgcaaag aattaattaa aaatatgcag ggcgatttat cattagaaag tcatccaggc
    11161 ataggaacaa catttacgat cacaatcccg gtagaaatta gccagcaagt ggcgactgtc
    11221 gaggcaaaag cagaacaacc catcacacta cctgaaaagt tgagcatatt aatcgcggat
    11281 gatcatccga ccaacaggct attactcaaa cgccagctaa atctattagg atatgatgtt
    11341 gatgaagcca ctgatggtgt gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg
    11401 cttattactg acgttaatat gccgaatatg gatggttttg agttgactcg caaactccgt
    11461 gagcaaaatt cttccttacc catctggggg cttacagcca acgcacaggc taacgaacgt
    11521 gaaaaagggt taagttgcgg catgaactta tgtttgttca aaccgttgac cctggatgta
    11581 ctgaaaacac atttaagtca gttacaccag gttgcgcata ttgcacctca gtatcgccac
    11641 cttgatatcg aagccctgaa aaataatacg gcgaatgatc tacaactgat gcaggagatt
    11701 ctcatgactt tccagcatga aacgcataaa gatctacccg ctgcgtttca tgcactagaa
    11761 gctggcgata acagaacttt ccatcagtgt attcatcgca tccacggtgc ggctaacatc
    11821 ctgaatttgc aaaagttgat taatattagc catcagttag aaataacacc tgcttcagat
    11881 gacagtaagc ctgaaattct tcagctgctg aactctgtta aagaacacat tgcagagctg
    11941 gaccaggaga ttgctgtttt ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc
    12001 taatgtggga gctatttacc agcacatctt acatttatga tgagaactcc tggcgaattt
    12061 gttccccatg ctggtcgagc gttgccgctc ccggcataac atgcgggtcc gcgcagccgc
    12121 tgatttttat cggattaccc ggcatcatta ttcctccggc ttcaatcaac atatttctcg
    12181 cctgagtttg tggcaaatta atggcctcag ccacacttaa taacggcgcg acgggcacac
    12241 caacttcatg tattttggct aaccaaactt cagctgcctg cgtttttaac gttcgctcaa
    12301 tatattgttt aagaatagcc tggttttgta cgcgtaaaat attgctgcta aatcggggat
    12361 cattaaccag ttccgtaagc tccagtgcct ggcataacgc agaaaaaagc ttgtcattac
    12421 cacaacaaat cgtaatcggc ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg
    12481 gatggcgatt tcccaggcgt tgtggtgact tccctgtcgc aatatatgcc atcagaccat
    12541 gctccagaaa actcagcgtg gcatcaaaca tcgctatatc gacatgcgcc cctctctggc
    12601 tcttttcgcg gccataaagt gcactcacta ttccgctgaa taaatagaca ccgccgcata
    12661 gatccgcaag agaggtacca acacgcactg gcggagcatc agggtaccct gtttccatca
    12721 ttatcccact cattgcctga atgatggtat cgtaggcagg agcatctttt agcggaccgg
    12781 tatgtccgaa acctgacgat gaagcatata tgaggcgcgg gttgatttct tgtaaccttt
    12841 cccatgaaaa ccccagtttt tccattgtac ctaggcgaaa attctcagct aatacatcag
    12901 cttgtttgag catatttata aatatacttt tatcgtgatc attctttaaa tcaagaacca
    12961 cactctcttt gccatgatta ataaaactgt aatagagtga ctgtccatcc acatagggac
    13021 caaatgtgcg ggtatcatca ccatgacctg gtggctcaac tttaattacc cttgcgccca
    13081 tattataaag aagttgagtt ccgaaaggtc cattaaggac atgtgtcata tcgataacta
    13141 ataagccttc aaacggccct ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa
    13201 gccgaaggtg aaaagacact tcggcattta tgtaaaatca ataacatgat taattatgaa
    13261 accagtcgac tgacataaat ccacaaggga gccgtgacga caaagcccag tacgctcaca
    13321 gccaatgacg ctgtaccagt acgggtgtag acattaaacc ggctggcaat aatgattccg
    13381 gagaatgccg gcggtaatgc gcctgccagt accatcatct gcaaatgttc gctgttcaaa
    13441 tgacatgcca taccaacgag aagcagcgcc agtggcatca gaatcaactt caggaaggtg
    13501 ttataagcaa tttcggcact gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg
    13561 aataccgcta cccctgagtt agccttcgca atcagattaa aggttgggtc ccatgctgcc
    13621 ggaattttta ccccaaccag caccaggatc gttgccagaa caggtgccca tactactggc
    13681 tcctttgccg cagaaattaa tgcgctcaga ttactattct tcttaccatc cgctcctgag
    13741 gaaggattca gcaaatacag accaatagga atagtaattg cgttaacaat aatagaaata
    13801 attgctacca ctaaacctgt tgatacggaa tcaccataaa taggatcgag aactgcaaac
    13861 cccaggaatc caatggtagg tgaacccgca attaatgcac atacagctgc ttctgcatgg
    13921 gtacgtttaa aaaatttgta gcaaccaaac caggagaaaa agaaacatcc gacaataacc
    13981 acaagtgata caagggtcag acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta
    14041 atagaaacaa atagagccgc aggaagagcg tagtttaata ccagtttatt aaatgcgcgg
    14101 gcttgatctt ctgaaaaagt ttctcgtctg ccgctaaaat aacccaacag catgattacg
    14161 ataataggca ataaatcgcc aataaaaaat gttagcataa tagtatttcc gataagggtc
    14221 ccctcaccat cgcataatga gttggcaaga ggagacgatg tcagtattta attaccagcg
    14281 acttgttttg ggttaagttt ggtaatatgg ccactttcag ttcctgctgc agggtcgata
    14341 accacattaa taatggtcgg tttgcgcgac tgaataccgg tggttaaagc atgacgaagt
    14401 tcatctgtcg tggtgacgtt atagccaacg ccacgaaacg catccattaa tttgtcatac
    14461 cttgcatggt gcaacagatc cgttggtgat ggtgcaccag cgccactgag atcaacaccg
    14521 tctcctctgt agatgccgcc attattaaaa ataacgatcg tcaccggcag gttatatcga
    14581 caaatcgttt caatttccat cccactgaaa ccaaaagcac tatcaccttc aatggcgaca
    14641 accggagaac cagaagtcac gctagcaccg atggcatagc ccataccgat gcccatgaca
    14701 ccccaggtac cacaatccag acgacgacgt ggtttataca tatcaataat atttcgtgca
    14761 ttatccaggg tatttgcacc ttcattaact aaataaatat cctggttctc gcgcaatacg
    14821 tcgcgcacag cacttaatgc attaaagtaa tttaatggtt gagtatctgt acttaatttt
    14881 tcatgcattt tttgtgcatt ttgctgcttg tggatattta aaatatcgcg ccataccagt
    14941 ggagtcgtaa atgtgttttg tttcagttct gccagcatac cttgcatact ggatgcaata
    15001 tcaccaacga ctggcacagc aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc
    15061 agttgaataa actgtgtatc tgccgcccat ccttttttac cgtgtgccaa taaccaattc
    15121 agtcgtgcac caacaagcat gacaacgtca gcatttgcca gggcaaacga acgcgcagct
    15181 gccgcagaaa gtggatgcgt atcttcaagg atccctttcg ccatagacat tggcaggaat
    15241 ggaatctgag cactttcaat aaattcacga agctgttcat cagcttgtga atacgccgcg
    15301 cctttgccaa ggataattaa tggccgttca gcttttgcta aaagcgaaat tgcgctagtg
    15361 actgacttcg ggcatggcaa taatgctggc gacggatttt caactttaac aatcgtggtt
    15421 aacgcttcgt ctttttccat cgtcgcggcc aggacatttg ctggcaaatc aagataaact
    15481 ccgccagggc gacccgatac agagacccgg atagcgcgtg ccaatgcaat gccaagatcc
    15541 tgcggctgat taacgcgaaa tgctgctttg gcatacggtt ttgccgcatt catttggtcc
    15601 agctcttcat aatcaccttg ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc
    15661 atgataatcg gaaaaccatt taccgttgcg ttggccaatg cggtcaaacc attgaggaat
    15721 cctggcgcag aaactgtcag gcagatcccc ggtttttggg taagaaaacc gcttgccgca
    15781 gcggcatagc ctgccgactg ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg
    15841 gcatggcgtg ccatatccgt cacaggaata cctacaacac cataaatagt gtcaatatta
    15901 ttctgtttta atgcttcaac gatgatatgc ataccatctg tcatttgaag ttgatctgac
    15961 atgattaata acctcgaaat ttttataaat tgggagccag aagcccccgt taatatcaga
    16021 tggcgtggtt ttgcttcatt gcagcaattt catcgtcgct ataacccagc tcctgcaata
    16081 cagcagcggt atgttcacct aatagcggcg cagctttaat atccggcgta aaggcagaga
    16141 atttcattgg acaaccaacg gtcagatatt ttccacgcaa cggttgttcc acttcaacaa
    16201 cactgccact ttggcgcaaa gagggatcaa gtgaaatttc tttcatactt aaaaccggtg
    16261 cacaaggaat atcaaactga gtcaaatagg ccaccgcttc atgtttatca atagtgacag
    16321 tgtatttttc gatttcagca aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac
    16381 tgtatgccgg atcggtaatc cattctggtt tgccgatggc tttacaggtg ttttcccagt
    16441 tttgctcctg aatagtgaaa taaatatagg cgttaggatc ggtttcccag cctttacatt
    16501 tcaggatcca gccaggctga ccgccaccac ctgcattacc accgcgggga actgcatcac
    16561 caaatgtacc attcggatac tgcgggtatt cttccagata acccaattta tcgagacgct
    16621 gctggtcacg taatttcacg cggcaaaggt tcaatacggc atcctgcatt gacatggtga
    16681 ctcgttgccc acgccccgtt ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca
    16741 aatgcattcc ggtgttgctg tcacccaacg ctgcagcgct taccagcggc ggaccatccc
    16801 aaaaacccgt agtggatgcc gcgccacccg ctgcctgagc aacgttttca taggctttta
    16861 cattcacata aggcgaacac tcatcaaacc ctttgatcga accaaaaatc agacgtggat
    16921 tgatttcttg aatatgctcc caggtgaagc ccatgtgatc aatggcccct ggatgaaagt
    16981 tctcgactaa gatatcagct tcgcggatca gcttttccat tacctctttg ccttccgctg
    17041 ttttggtatt taactcaata gaacgtttgt tactgttaag catggtgaag taaagcgcat
    17101 cgatatcagg aatatctcgc agttggtgac gcgttacgtc accaacgccg ggacgttcaa
    17161 ttttaataac gtcagcgcca aaccaggcca gcatttgagt acaagatggg ccagattgca
    17221 caccggtgaa atcgagaact ttaattcctt gaagtggagt tgacatagca atacctatta
    17281 gttaattaaa tatgtgttaa tttagcgttt atttaaggca taacgccctc tataaataat
    17341 ctgcaaaata tcttcgtagc ggcactcgat atttttaaat tcctgtatca agtactgaaa
    17401 attattttat cgccacttcc aattcctttc tgcatggtaa gtagtgagga cgaaaagaag
    17461 aatatgataa atattgattc gtggtaatca gagagagtcg cgacatttgt gatgttattc
    17521 ttacaactgt aagaagcgcc ggtgatttac aagtaagagt atgattacgc tttttaaagc
    17581 gtaccataaa gcataacata ataaatgatt tgtgatgaca cgggattatt gcggtttact
    17641 ttgaggaaaa taaaatgcgt gtttatagaa ccgcgagcgc caaataattt tatgaccaca
    17701 acctgtcgta ttgtggtcat aaaaaattgt cggctggagg gagttaaatc ctttacctca
    17761 atatactgcc tgtcagcatt agttcacaaa cagtgcttcg ctatcgacga tgattccatc
    17821 ttccgcacct ttaagcgcca ggtttgcctc gtagtattgc tttttatcaa gcagtttctg
    17881 cgcatcagca agtgcattac gagtctgttt aagcggcatc agatattggt tttccattac
    17941 gccaacaccg gccaggcgaa gctcttccat tgcgcccttt ttatcccctt tcgccatttt
    18001 ttcattggca atttttatcg cggcttcttt ttcgggtgtt gctacataac tctcagaaat
    18061 tccgacagat gcattaatga caatatattg gtcatcattg agattggtct ttttacccgg
    18121 cttagcgaac tttgcccatt cggtactgtc atctgacagc aaagcggaag cttcattggt
    18181 cagttctttt gccttttccg gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc
    18241 acgcatcgca tataaacctt gttcagagat acgctctgct gctgccgttt tctgggtttg
    18301 ctgagtttgc tgttgtgccg ctaccggtgc attatcagca gcccatacag ttgaagatgc
    18361 cagaattgct gtgaccatcg tagccataat taaacgtttc atacttacct cattctttaa
    18421 cagttttaga aaacgccgcc atcatattgg gtgagcatga aatgagaaat cagagagcaa
    18481 cggtcatttt gaaagggtta tcttacaatt gtagttatta tcgcaaagcg gcatttagtc
    18541 ttacagttgt aggaatatgc ctcctgtcgc agagaactat tcttatatac cacaaagaga
    18601 tgtcatttat tatattgatg taaatattga ttaattatat gtaaatacag ccataacatc
    18661 gtatggttct cagtgaacca ttttcaggat aactttctat gaaagtaaac ttaatacttt
    18721 tcagcttatt tttattggtc tctattatgg catgcaatgt ttttgcattt tccatttctg
    18781 gtggtgtaag tgagaggagc tataaagaga ctgaaaaaac atcagcgatg acgaccacac
    18841 actctacaaa acttcagcca tcacaggcga ttttgtttaa gatgagagaa gatgtgccac
    18901 cattaaacct cacagaagaa atcacgccta cttatccgac aaaggcgaat tatcttattc
    18961 atcctgtgcg atagcgtctg tcacatggca ggtgaacaat catgtctgat gctgatgtga
    19021 acacctgccc aaatgagcca tcctgaccca ctatcgcctg ccatacccca ccataaagcg
    19081 aatcatcaag ccacaagccg gggcaagaaa cataaatatc cccgtcacca gttcattacc
    19141 gccagttaac cacatggtga agtaataaat gagtgcagcg aggatcacca tgccgaagaa
    19201 agaagagacc gccgatacca cgttcagtac ctgaccgatg tagccgctta cgcagacaat
    19261 acacagggcg agaaacatcc ataaataaat catagctcaa cctcctgccg cgctaaaaaa
    19321 cctgtaactt cagtcacttt aatagtaaga caatccttaa cctctggcaa tttttgctaa
    19381 gcatcaatga aaacagcacg ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt
    19441 gcacgattaa taatcagtag ctgaaagcag tcagccagtg ataaagtaga gccaccgcat
    19501 tcatgctcct cgtggttatg tcctgactag tctttacact ctttacagga accattgtcg
    19561 tacatgatgg cccaaccaat tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa
    19621 caattcgtat ctacagaagg taactatgtt tccacaatgc aaattttccc gcgagtttct
    19681 acatcctcgc tactggctca catggtttgg gcttggtgta ctctggcttt gggtacagct
    19741 tccttatcct gttctctgct ttctcggcac gcgtattggc gcaatggcgc gaccattcct
    19801 gaaacgtcgt gaatctatcg cccgtaaaaa cctggaactt tgtttcccgc agcattctgc
    19861 ggaagaacgc gagaagatga ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga
    19921 aaccggcatg gcatggttct ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg
    19981 gttggataac cttaaacgcg cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca
    20041 ttttatgtcg ctggaactgg gcggccgcgt gatgggactg tgccaaccaa tgatggctac
    20101 ctatcgtcca cataataatc agctgatgga atgggtgcag acccgtgggc gcatgcgctc
    20161 taacaaagcg atgatcggca gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg
    20221 tgaagcggta tggtttgctc ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc
    20281 gttctttgcg gtggaaaatg tcgccacaac caatggcacc tatgttctct cccgtctctc
    20341 tggcgcagcc atgttgaccg taacgatggt aagaaaagcg gattacagcg gatatcgttt
    20401 gttcatcacc ccagagatgg aaggctaccc gacagatgaa aatcaagccg ctgcttatat
    20461 gaacaagatt atcgagaaag agatcatgcg cgcaccggag cagtacctct ggatccaccg
    20521 tcgctttaaa acgcgcccgg tgggagaatc gtcgttgtac atttaacaaa ctgctcttac
    20581 tctttccctc ctcaaatcgg ccatgataac ctcatgattt ttatagcttt tttcttaagt
    20641 tttttccttc gctgaatatc taattattac cgtcagaaat taaactgtcg ccgatatgag
    20701 acacttgtaa gttactaaaa gtaaataaga aaatgcctct tgtctcgttt cagttttagg
    20761 cgtacattag cgccgtctcg agcagtttag ctcagaattc tggggtgatg cccacggtaa
    20821 aatacagggc ttttaaggtc gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa
    20881 gtcattcagg cgtaacagta attacgcgga gagatgtaaa gtgaaatatt tctttatggg
    20941 catttctttt atggtcatcg tttgggccgg tacttttgcc ctgatgatct aaaagcaaac
    21001 tgtgcaatga aaaacaggag ccatcaggct cctgtttgca tttctgacga cggtgtttat
    21061 tccgcgtttt cgtgaatatg tttgctgctg acgggtagta aaccgtcggc acggaacatc
    21121 gctttaatcc ctctgatggc ctgacgaata cggtcgtggt tttcaatcag tgcaaagcga
    21181 acatgcgtat caccatagtc gccaaagcca atccccggcg agacacaaac cttcgcttcg
    21241 ttaaggagct tcttggcaaa ttccagcgat cccatggccg catatggttc cgggattttc
    21301 gcccagacat acatcgaagc cttcggcatt tcgaccatcc agcccgcttc atgcagccct
    21361 ttaaccaata catcacgacg gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga
    21421 tcgccctcga gtgccgcaat cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga
    21481 tagcttttga ttcgcgccag ggcactgacc agcgttttgt tgccgaccat aaagccgata
    21541 cgccagcccg ccatgttgta gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt
    21601 gcacccggca cctgcataat tgacggcgct ttccagccat cgtagacgat atcggcatag
    21661 gccaggtcat ggaccaccag cacatcgtag catttcgcca gcgccacaac cttttcgaag
    21721 aactccagtt ccacgcattg cgcggttggg ttcgacggga agccgaggat catcatcttc
    21781 ggtttcggat aactttcacg aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc
    21841 accagcggca ctgagcgtac ctgcgccccg gcaatcaccg cgccataaat atggatcggg
    21901 taacttggat tcggcaccag caccgtgtca ccatgatcca gcgtcgccag catcagatgc
    21961 gccaggccct ctttcgaacc aatagtgacg atggcttctg attccgggtc gatttcaacg
    22021 tcgtagcgat cctgatacca gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa
    22081 gtggagtaac catgcgtgtc cgggcgctgg gccacagtac ataatttttc gacgatatgc
    22141 ggcggagtcg caccgtccgg gttacccatg ctgaaatcga taatatcttc gccgcgccga
    22201 cgcgcagcca ttttcagttc agcggtgatg ttaaaaacgt agggcgggag acgatcaatg
    22261 cgcgtaaagc gacgttcagg gcgagtgtca gccataattt tctcagatta acgttagcgc
    22321 ccggaccgtc cgagcgacgc tgccacaatg gtggctgttt tgaaaatagc ctgattaatt
    22381 tctgtctgtc cagcgtttta gaacattttt atcaatggat ttaaatgagg aactccactt
    22441 caaaaacagg aaatttttct acgctaacaa gaaaatcacc accaatttta ttaacattat
    22501 aatttcaata aatttaccag ctagaagtgc attatcaaga gatacattgc taatacacca
    22561 tttatctttc aatccccttt taaatttatg gtttttcctc aaaaacggcc tgctggtcat
    22621 cagccttcag gttacctatc atagaggttt aatccttatt cagagtcacc cgtgcacgaa
    22681 atattcaaca tgctgctggc ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc
    22741 tttctcatcc gtatccgcct gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa
    22801 gaattgctcg ccgttaccgc cattttctcg ctgttcgccc tgttcagcac ctggtccggc
    22861 gttcccgtag aaggctcgct ggtgaacgta cgtattatcg cggtgatgtc cggcgggatt
    22921 ctgtttggcc cgtgggtagg catcattacc ggcgtgattg cgggtattca ccggtattta
    22981 attgatatcg gcggcgtgac ggcgatcccc tgctttatca ccagcattct ggcgggttgt
    23041 atatcaggct ggataaacct gaaaatcccc aaagcacagc gctggcgctg gatatcgcca
    23101 acaaaacgct accgctgttt cgccatgtca atagcgagtc attacgcaag gtctgcgaaa
    23161 ttatccgcga tgacattcac gccgatgcgg tggcgattac taataccgat catgtgctgg
    23221 cctatgttgg cgtgggtgaa cataactatc agaatggcga tgacttcatt agcccgacta
    23281 cccgtcaggc gatgaattac ggaaaaatca tcattaaaaa caatgatgaa gcccaccgca
    23341 caccagagat tcattccatg ctggtgatcc cattgtggga gaaaggggtc gtgaccggaa
    23401 cgctgaaaat ttactactgc cacgcgcatc agatcacctc gtcattacag gaaatggcgg
    23461 tcggtctgtc gcaaatcatc tccacgcaac tggaggtttc acgcgccgag cagctacgtg
    23521 aaatggcaaa taaggcagag cttcgcgccc tgcaaagcaa aattaatccc cattttctgt
    23581 ttaacgctct gaacgctatt tcatcgtcaa tccgtctgaa tccggatacc gctcgccagt
    23641 tgatctttaa tctgtcgcgt tatctgcgct ataacattga attaaaagac gatgagcaaa
    23701 tcgatatcaa aaaagagctg tatcaaatta aagactatat tgccattgag caggcccgct
    23761 ttggcgacaa gctgacggtt atctatgata ttgatgaaga ggtgaattgc tgcattccca
    23821 gcctgctgat ccagccgttg gtggagaacg ccattgtcca cggtattcag ccttgcaaag
    23881 gtaaaggcgt tgtcaccatc agcgttgcag agtgcggaaa tcgggtacgc attgcggtgc
    23941 gagataccgg gcacggcatc gttccaaagg tgattgagcg ggtcgaagcg aatgaaatgc
    24001 cgggcaataa aattggcctg ctgaatgtcc atcatcgcgt gaagttattg tatggcgagg
    24061 ggctgcatat ccgccgcctg gagccgggga cggaaattgc gttttacatt cctaaccaac
    24121 gcaccccagt cgcctcacag gctacgttat tgctttgagc cggagtgata ttgtgaaagt
    24181 catcattgtt gaagacgaat tcctggcaca acaggaactg agctggctaa ttaaagagca
    24241 cagccagatg gagattgtcg gcacctttga cgacggtctg gacgtgttga agtttttgca
    24301 gcataaccgc gtcgacgcca tttttctgga tatcaatatt ccgtcgctgg atggcgtgtt
    24361 gctggcgcaa aacatcagcc agttcgccca taaaccgttt attgtgttca tcaccgcgtg
    24421 gaaagaacat gcggtagaag cgtttgaact ggaggcgttt gactacattc tcaaaccgta
    24481 tcaggagtca cgtattaccg ggatgctgca aaaactggaa gcggcctggc aacaacagca
    24541 gaccagcagt acgcctgccg cgacggtaac gcgtgagaat gacaccatta atctggtgaa
    24601 agatgagcga ataatcgtca cgccaattaa cgatatctat tacgccgaag cgcacgagaa
    24661 aatgaccttt gtctatacgc ggcgtgaatc ctacgtaatg ccgatgaaca ttaccgaatt
    24721 ttgcagcaaa ctgccgccgt cgcatttttt ccgctgccat cgctcatttt gtgtcaatct
    24781 gaacaaaata cgcgaaatcg aaccgtggtt taataacacc tacattctgc gactgaaaga
    24841 tctggatttt gaagtgccgg tcagccgcag caaagtgaaa gaatttcgcc agttaatgca
    24901 tctttaatcg ggaactttca tgaaagcacc aggcttgccc gccgatcaac aatttttcgc
    24961 cgatctgttc agcggcctag tgcttaaccc gcaactactg gggcgcgtct ggtttgccag
    25021 ccagcctgcc tcgttaccgg tgggcagttt atgtattgat tttccccgtc tggatatcgt
    25081 gctgcgcggc gaatacggca acctgctgga agcaaagcag cagcgaatgg tggaaggaga
    25141 aatgctgttt attccggcac gcgcggctaa tttaccgatc aacaacaaac cggtgatgct
    25201 gttaagcctg gtgttcgcac cgacctggct tgggttatcg ttttacgata gccgcaccac
    25261 gtcgttgttg catcctgctc gccagaccca gcttcccagc ctgcaacgcg gtgaaggtga
    25321 agcgatgctt accgccctca cccatcttag ccgttcgccg ctggagcaga atatcattca
    25381 gccactggtg ttaagtttgc tgcatctttg ccgcaacgtg gtgaatatgc cgccgggtaa
    25441 ttcacaaccg cgcggcgatt ttctctatca cagcatttgt aactgggttc aggataatta
    25501 tgcccagccg ctcacccgcg agagcgtggc gcagtttttt aatatcacgc ccaatcatct
    25561 gtcaaaactg tttgctcagc atggaacgat gcgttttatc gagtatgtgc gttgggtgcg
    25621 aatggcgaag gcgaggatga ttttgcagaa atatcatctg tcaattcatg aagtggcaca
    25681 gcgttgcggt tttccggata gcgactattt ttgtcgcgtt ttccggcgtc agtttagtct
    25741 gacgccggga gagtacagcg cccgttttca gggctaacgt cagaaggtta attctgtttc
    25801 cagcagcgtc aggatacttt gtgcatcttg cgcggcaaac aacgactggc ggaagttttt
    25861 attcaccagt ttacgcgcca gctgcgagaa gactttcaca tgattcatcc cttcgttagc
    25921 acccagcgtc agcatgatat tctccagcgc cagcagtggg cgaacgtctt cttccggcgt
    25981 aaaggcggtg agtaacgctt caatttcctg cgcactgcgg cattcacatg cctgacgcgc
    26041 cagttcccga cacgcctcgc tatccagttg acgaagctgg cttttcaccg ccggaatacg
    26101 cgggctactc atactcagct catccaggcc cagcccaagc agtagcggca gataacggct
    26161 ttcaccgccc agttcaccgc aaatgcctac ccatttgccc cgctgatgcg cagtggtaac
    26221 tatttgctgc aacatgcgca ggaacgatgg cgtaatcggg ttatatagcg gcgatacgcg
    26281 cgggttatta cgatcgaccg catacagata ctgggtcata tcgttggagc cgatactgaa
    26341 gaaatcgacc tcatcgcaga agtggtcgat gatgtagcac accgacggca cttccaccat
    26401 gatcccaagc gtaatcgtct ctgcatgacg caggccatcg cgcttaagct caacgatcgc
    26461 tttttggatc tcgcctttca cccataagat ctgatcgagg ctgtgaacca tcgggatcat
    26521 caactgggcg ttgccgaaac tggcggcgcg caaaatggcc cgcagttgag tgcggaacag
    26581 gccagcaaat tccgggtaaa tacgtaccgc gcgatagccg aggaacgggt tctcttcctg
    26641 gggaatattc agataaggaa tgcttttatc gccgccgatg tccatcgtgc ggaagataat
    26701 cggcttgtcg cccgccgcca gtagcacctg ctggtaggct tcaaattgct cctgctcgtc
    26761 cggcgcgctg tcgcgatcca tatagagcat ttcggtacgg aacagcccga caccttccgc
    26821 gccgttggca aacgcgcctg gcgcttccag agcggtgccg atattcgccg caatatcaat
    26881 acgcttgtta tcacgggaat aggccagctg cgcggcagcc tgcgcctgtt gtttttggcg
    26941 tttatccgcc agcgtctgcg cgacctgata ataaccgctc accgcgtcat tcgggttaat
    27001 cgccagcacg ccgcactggg cgtcaagcac tgcaggttgc ccggcataac gggcaatcgc
    27061 atccagcggc aagccactca gtaccgggat cgccgaggca cgggccagaa tcagtgtatg
    27121 cgaggtgcgc ccggttttct ccagaatcat acccgcaaga tttttcaaat caaggctcaa
    27181 aaactggctt ggggttaaat cttcagccac cagaatggtc ggtttttcca gcaccagatt
    27241 gttgcgcggc ttcagttccg gccaggtgat atgcagcaac tgttcgctga tatcgcgaat
    27301 gtcgctgacc cgctcgcgca gataatcgct ggcagaagca gatagtttgg cgcaaacctg
    27361 ctccatattg ctgatgatcg ccgcccccag tccctgatgc tgttctgtca tcaggcgacg
    27421 gatattgcct gcaaattcat catcctgaat cagcgacaaa tgagcgctga ggatagtttt
    27481 gctttcgccg tcacgctcac gcaattgctg attcagttgc tcggcaagcg ttgccaggct
    27541 gtgctccagc cgggtggaat cttgcgcact ggcggggatt gcccgataac tgtcgaggct
    27601 gtcgctttgt aacagggtca gcgtacccac gccgacgccg cttgccagca cattgccgta
    27661 cagtaaatcc gggttaaggc ggcttaatga acgcggcagc ggatgcgccg tcagttctgc
    27721 ctgcgtcggc tgaacgctgt cgctgtcgat aaagcgcacc aggatgtact cttccagcac
    27781 gcgccgcgcc tgctcttcat cgctgccgct aatgttcagg ctgcaactgt cattaaataa
    27841 ggtgccagtt ccaatcaacg ccagcgagct tttagcatca gcctttgcgt tctggcgatg
    27901 gttgataaag gtgatttcgc tttgccactg gctgcactgt tctttaagtt cccacgccgg
    27961 acgagcatgt agaccgttag gcaaaggaca gagaaattga atcgttaaca taaccgctcc
    28021 gggttaatca ggagatcatc tgaaatccgt cagttgaaca accgtctcac gcgtaagacg
    28081 ttgaataagg gcagataaaa gttgctccat ctgcaaaatg tcgcggcaat cggcaatcga
    28141 tgccgcgcaa tgtccgtggc gggtggcagg ccctattacc aatgtgggta cgccagtacc
    28201 ggttaagtgc accgctccac cgtccgtgcc gccgttactg aacatatccg cctgcaacgg
    28261 aacgccaatt tctgccgcca cggtttcaat ccacgcggta agttttggcg gcgcaatcag
    28321 cgatttatcg cttaacacca gcatcggccc gttaccaatc tgacgatggt tagccgcgcc
    28381 ataatcaaag tttttcgccc agcaggcggt atcaagcaca atggcgacgt ccggcgacac
    28441 cgcgcgggta gcagtttgcc cgccgcgtaa tcccacctct tcgctggaac ttgccaccag
    28501 ccacacttcc gcaggcagtt cggcgtcgtg cagctcacgt agtaacgtca ccagcagata
    28561 gcaactgagg cgatcatcaa aggctttgcc catcacccgc tggtgaggga ggacctgaaa
    28621 agtggtatcg aaggtgacgc gatcgcctgg acgaattccc gcctgcatca cttcgtcata
    28681 ggtgcgcgcg ccaatgtcca cgcgcatggc gctaacgtca ttcccctgcc ggtcgccgtc
    28741 aagcaggcct ggaattttgc actcttcacg ggtggtgatg cgcaccggct gcagctggcg
    28801 ggcagccatg cgcacgttgc caaccggcag cacgtcaata gcgccttcgc gggagatgct
    28861 gcgcaccata aatcccactt cgtccatatg ggcgcagatc atcaccttcg gacctgtcga
    28921 ttcattgagg cggatcagca ccgatcccag accatcaaat cgcacttctt tttgcaggcg
    28981 agccgcttct tccagcagga tctgccgcac ttcctgttcc gaggaggcga tcgcatctgc
    29041 ctcgctcaac gcttttaata gcgataaatc cattatgcct ctcccgtgag caacactgtt
    29101 ttcggcatgg cgtagagcac ttctgcgcct tgcggggtaa ccagcacaac atcttcgatg
    29161 cgcacgcccc cttgccctag caaataaatc cccggctcca cggtcagcaa catgcctggc
    29221 tgtagcgtcg tggtgtcccg cggtgaaaaa cgcggatctt catggacttc aatgccgata
    29281 gcgtgaccag tgttatgacc gaaatagtcg ccaaatccag cctcggtaat gacccggcgc
    29341 gcggcgtcgt caatctgctg gcagcgcacg ccggggcgaa ttgcagagat tgccgcgagc
    29401 tgtgcctgca ggacaatctg atagacgtca aacagcgggt gagattcggc gctcacccct
    29461 tcgccattca ccagcaaggt gcgcgtcata tcagcagtag ccctgataca gcgcaccaaa
    29521 atcgagagtg acaaactcgc ccgctgcaac aatcttgtcg ctggctttgc cgtgcggcag
    29581 cgccccgcgc cagccgctgg cgacaatggt gtcaaaagag gctttttctg cgccctgctg
    29641 gcgcataaac cactccagtt cagcggctat ctcgcgctcg ctcatccccg cctgaataaa
    29701 gcggcgaata tgctccgctc cgcgatcggc aatcccacag gcgaggcgga ttatctccac
    29761 ctcctctggc gttttgattt gccgcagcac atccggcgtg gcgctaacca gtttcgcatt
    29821 gagttcagac ttccagcggt gcgcggtttc ccaactcacc tgctggcctt caaaaccgag
    29881 cgtttgcaac tgctcatcgg caatgatttg attgacgata gtggtaagcg tgttcgtcgc
    29941 gtcaagcaaa tgcagctggt agccttgcgc acgggcttcc acctcaacga aatagcgcga
    30001 atctaccaga atgtgcgcac tttcacggct aataaccaca tagcctgatc cggtggagat
    30061 ccccagatgc ggctgtttgt tctgccgtga ggagagaagc accgcatcca gttgttgcac
    30121 cttaagccag ttgcgcagcg aagcgagtaa tgtcatctgg attttccttc ttacaggcta
    30181 tcgattaaca atttgccttt gcggaacatc atcagacgca ggaacaccac catcagcgca
    30241 gtaatgattg cccccagcgc gatccccgcc atataaacgc ccaggttagt gaccagcggc
    30301 catgcccaga tagcggattc cgggaaccat tgcactgcgc ccagccagac agctgcggtt
    30361 gagccgacaa tcgcgccgac catatatgac ggaatagcgg tgatggggct ttccagcgca
    30421 aacggaatcg caccctcact aatccccata aaggccagga acatcgcggt cttaccctgc
    30481 ggataaagtt gagcgttgaa caggcgtttg ccggttaaac gacggtcaat aatggtcgcc
    30541 agacccagac caatcggcgg aataacgata gcgatagaac gagcggtgac cggtaatacg
    30601 tggtcagtgg taaagctgaa ggcaacgaaa cctgcggctt tgttgatcgg gccaccaagg
    30661 tcgattgctg tcgccgcggc gatccccatt gcgtacatca gcgcgccctt ctcacctgcg
    30721 gcagtcagca cagtacggat accgccgttg atccagccac caaagggagt gatgacgtag
    30781 tacatcgcca gcatgacaaa aatggcggaa agaatcggta gcaaaaatgt ggttttgaac
    30841 gctaacagga aatccggtaa ctggattttc tggttcatcc atttcacaag gtaaccggca
    30901 acgatagaaa taatcagcgc accaatgaag gtggacggca ccggcgaaga ggtcgcccat
    30961 tgcatagtgc tgggatcgaa gttcagcagt tgcgtcggct gggtagacat caacccgccg
    31021 ataaaaccag ccgggaacgc cagtttgcca ccgatagagt tcgccacaaa tgcagcaaac
    31081 atcggaatgg caaaaccaaa caacacgccg ccgaaggact gcgacagcca ggcaaattta
    31141 agtaaggaga ggtcgaaacc ggagaatttc ccgctattaa gagcatccat gatgccgatc
    31201 tcagcaggaa ttttaagcca gctataagca atcagctggc tgaaagcgag gatcacaccg
    31261 cccataatta aggtcggcac catgcgtgaa atccctgaca tcacatgctg cgggagttca
    31321 ccccagaagc tgcttttaga ggcctgcgga ttcttaactg ccgctgccgc accggatgcg
    31381 ccaggcacaa cggttgcact gcgtttttta atggccataa caattatccc tgtcgaatat
    31441 tactgttgtt cagaggcaat catctcttcg atttctttga tgatgcccgc agcgttttta
    31501 attgcgtcct gcaaagtgat ttcataaacg tcgcgtgatt cgaaacgttc gttatcttcc
    31561 ggggtaactg ccacggaatg gatgatgatg gtcgcttcgg cgatatcctg cgccgtcagg
    31621 cgattctgga tgccgtccgc gccctgggtt tcaattttca cttcataacc ggcttctacc
    31681 gccgcttctt ccagcacctg agcggccata aaagtgtgag ctaggcccat tgggcaggca
    31741 cataaggcaa tcagtttctt actcatcgtt aattcctcgt tcagtgatag ttgacgagtg
    31801 aaattatgaa cgtgcaaaac aaatcgccgt taccagacaa ataatgcatt tactggagaa
    31861 ttaatcccgc tgtacggaag tgtgacgcag gtcggccttg tgaggggaca caaagaaaaa
    31921 ttgaattaac tgtatgattt aaaagattat cgggagagtt acctcccgat ataacaggaa
    31981 ggatttacag aatgtgacct aaggtctggc gtaaatgtgc accggaaccg agaagacccg
    32041 gatagtcatg gacgatgaga tacaccggaa tatcatggac atattcttta aagcgccctt
    32101 tatcttcaaa tgcggcacgg aaaccggagg ctttgaagaa ctcaaggaag cgcggcacga
    32161 taccgcccgc aataaacacg ccgccaaatg tcccgagatt gagcgccaga ttgccgccaa
    32221 aacggcccat aatgacgcaa aacagcgaca atgcgcggcg gcaatcggtg cagctgtcag
    32281 ccagcgcgcg ttcggtaata tcttttggct tgagattttc tggcaggcgg ttgtcagctt
    32341 tcacaattgc gcgatacaaa ttcaccagcc cagggccaga aagcacgcgc tccgccgaaa
    32401 catgaccaat ttccgcacgc aatatttcga ggataatggc ctcttcttca ctattcggcg
    32461 caaaatcaac gtgaccgcct tcgcccggca agcttaccca acgcttatcg acatggacca
    32521 gatgcgcaac cccaagcccc gttccggcac cgtaaaccgc aataggctta ccttcgaccg
    32581 gttctgcgcc accaaactga atcagatgct cttttttcag catcgggatc gccatcgata
    32641 cagcggtaaa atcgttaata atttccagat ggctaaaacc gagattcttt ttcatttcgg
    32701 caattgagaa cgcccaggta tggttggtca ttgccaccca gtcaccggta attgggcaag
    32761 cgatggcaat acagccgtct ttcacctcga ccttatgttc ttcaagataa acgcgaatga
    32821 ccgcttcgag gctggggtaa tcaagccctg aataggtctt agcctgcgag atttcaccac
    32881 tggcaatatc acacagagca agacgtgcgt tggtgccgcc cacatcaccg actaatgcat
    32941 actttgtcat tcttcaactg ctccgctaaa gtcaaaataa ttctttctca cactgtaaat
    33001 acctgggggc ataacaacaa cgccggaaag gcaggctccc tgtaaatatc gatctgggtc
    33061 acacaattac tttatcgttt cagcaccaat tgcagcgatg cctttttgca agctgggcaa
    33121 actaagtatc tgaccccgca taaggaatag aacatgctcc atccgcgagc cagaaccatg
    33181 ttgttattat cgctccccgc cgtggcaatt gggattgcgt ccagtcttat tctgattgtg
    33241 gtgatgaaaa tcgcctcggt attacagaat ttgctctggc aacgactgcc gggaactctg
    33301 gggatagccc aggattcacc cctctggatc atcggtgtat taacgctaac gggtattgcg
    33361 gtggggttgg ttatccgttt cagccagggt catgccggac cagaccccgc ctgtgaaccg
    33421 ctgatcggcg caccggttcc gccctctgcg ctacctggac ttatcgtagc attaattctc
    33481 ggtcttgctg gcggcgtcag cctggggccg gaacatccga tcatgaccgt caatatcgcc
    33541 cttgccgtcg ccattggcgc tcgtctgtta ccgcgcgtca accgaatgga gtggactatt
    33601 ttagcctctg ccggaaccat cggcgcgctg tttggtacac ctgttgcggc ggcgttgata
    33661 ttttcgcaaa ccttaaatgg cagtagtgaa gttccgctat gggatcgtct ttttgcgccg
    33721 ttaatggcgg cagcagctgg tgcacttact accggattat ttttccatcc ccatttttca
    33781 ctgcccattg cgcattacgg acagatggaa atgaccgata ttctcagcgg tgcaattgtc
    33841 gcggcgattg ccatcgcagc agggatggtc gccgtatggt gcttaccacg gttgcacgcg
    33901 atgatgaatc aaatgaaaaa tccggtgctc gtgctgggta ttggcggatt tattctcgga
    33961 attctggggg ttattggtgg accagtttcg ctgtttaaag ggctggatga gatgcagcag
    34021 atggtggcaa atcaggcttt cagcaccagc gattactttt tgctggcggt aattaaactt
    34081 gccgccctgg tcgttgctgc cgccagtggc tttcgcggtg ggcgaatctt cccggcagtg
    34141 tttgtcggcg tggcattagg gttgatgctg catgagcacg ttcccgccgt tccagcggca
    34201 ataaccgttt cctgcgctat tctcggcatc gtgctggtgg taacacgcga tggctggtta
    34261 agtcttttta tggcggcagt cgttgtaccc aataccacat tgctaccgct gctctgtatc
    34321 gtcatgcttc cggcatggct gttattagca ggtaagccga tgatgatggt caatcgtccg
    34381 aagcaacagc caccccacga taatgtttag caaaaaatgc tctcctttat gatttaagag
    34441 ttatggcgat gatacggggc tttacctccc cgtaatattg cgttaacagg ccgctgacgg
    34501 cttcgatcgt gaaggagaat aacaatgttc aggtcactgt ttctggcggc cgccctgatg
    34561 gcatttaccc cgcttgcagc aaatgcaggt gaaatcaccc tactgccatc gatcaaatta
    34621 caaattggcg atcgcgatca ttacggtaat tactgggacg gtggtcactg gcgagatcgt
    34681 gattactggc atcgcaatta tgaatggcgc aaaaaccgct ggtggcgtca tgataatggc
    34741 tatcatcgtg gctgggataa gcgtaaagca tatgagcgtg gctatcgtga aggctggcgc
    34801 gatcgtgacg atcatcgcgg aaaaggccgc ggacatgggc accgccatta aacgcgtcct
    34861 ccaatggagc acaatgcctg atgcgacgct agcgcgtctt atcaggccta caaaccggct
    34921 ctttaacgcg tagggcggat aaggcgttta cgccgcatcc gccaatagtg ccagatgcga
    34981 cgctcattca actacaatcc cagcgccgtc cccaccaaca accagatatt cagcgccacg
    35041 accagcacta caatcaccca gcctgtctgt tttacgcgtt tgctgttcac cagatcgccc
    35101 atcaacttgc tgtcactggt gaaaatcagc agtggaacca gcgccagggc gataccaaaa
    35161 cttaacagca cctgactcat aaccagaatc cgtgtcggat ctaatcccat cagaatgaca
    35221 ataaatgacg gcaacatggt gactgtacga cgcacccaca gcgggatatg gaagcgaatg
    35281 aatccctgca tcaccacctg ccccgccagt gtccccacca ccgttgagga cagtccggca
    35341 gcaaccagac ttaacccaaa gaccgttgcc gcagcatggc ttaacagcgg ttgcagcgtc
    35401 agataagcct catcaagatc ggcaacacca gtatgaccag aaaagtggaa cgccgccgca
    35461 gctgtagcca tcatcaccag attgacaaaa ccggcaatcg tcatggcgat agccacatcc
    35521 catttggtgg cggaataacg ttgttgacgc gaaccgccat gtaaatgctg agtgagcgaa
    35581 gagtgcaaat aaatcacatg cggcataatc gtcgccccta acacgcctgc tgccaggaag
    35641 accgcctccg aagtaggtaa actcgggatc accattcctt tacccagctg cgccaggtta
    35701 ggctgggaga aaatcaactc gacaatgtaa gccgcggcaa caaacaacag taacccgcca
    35761 atcactttct ccagcggttt ttgcccgcga cgttgcagca ttaaaatcag gaaagtcgcg
    35821 atccccgtca gcaccgcgcc ctgcaacaac gaaacaccaa gaatgagttt aaaaccgatc
    35881 gccgcaccaa taaattccgc caggtcggtt gccatcgcaa taatttctgc ctgaacccaa
    35941 tagaaccaca ctacgggacg cggatagtga tcacgaatct gctccgccag atttttaccg
    36001 gtggcaatcc ctagtttggc agagaggatc tgaatcagca tcgccatcag gttggcccaa
    36061 acgacaaccc acagtagctg atagccgaag ctagcacccg cctgaatatt ggtcgcaaag
    36121 ttaccgggat cgatataacc aatcgccgca atgaacgcag gtcccattaa tgcgagcctc
    36181 atcttgcgcg ccgcccgtcc gctgctactc tcaacgcgat agttcgtcat cttgtgcctc
    36241 taaaacatag cctttgctat gtttcatgct atgctaaacg agaatgatta tcaaattcat
    36301 ttaaatggat tgtggtgatt tctctgatag accaagatta tgattacgaa aagattgatg
    36361 acttgaatgt tatggttatg tttaatgtta gcacatttac ataactttca gcttccatac
    36421 acaacatagc agaaatgtat gacagatcac tatttttgaa gcctgtcaca ggacgtcatt
    36481 atagtgtgtg tcagatctcg ttttccttaa ccatgttaca tagaatgtgc acggaaattt
    36541 aacctgcctc atatttggag caaatatgga ccgcgtcctt cattttgtac tggcacttgc
    36601 cgttgttgtg attctcgcac tgctggtaag cagcgaccgc aaaaaaattc gtatccgtta
    36661 tgttattcaa ctgcttgtta tcgaagtgtt actggcgtgg ttcttcctga actccgacgt
    36721 tggtttaggc ttcgtgaaag gcttctccga aatgttcgaa aaactgctcg gatttgccaa
    36781 cgaagggact aacttcgtct ttggtagcat gaatgatcaa ggcctggcat tcttcttcct
    36841 gaaagtgctg tgcccaatcg tctttatctc tgcactgatc ggtattctcc agcacattcg
    36901 cgtgttgccg gtgattatcc gcgcaattgg tttcctgctc tccaaagtca acggtatggg
    36961 caaactggaa tcctttaacg ccgtcagctc cctgattctg ggtcagtcag aaaactttat
    37021 tgcctataaa gatatcctcg gcaaaatctc ccgtaatcgt atgtacacca tggctgccac
    37081 ggcaatgtcc accgtgtcga tgtccatcgt tggtgcatac atgaccatgc tggaaccgaa
    37141 atacgtcgtt gctgcgctgg tactgaacat gttcagcacc tttatcgtgc tgtcgctgat
    37201 caatccttac cgtgttgatg ccagtgaaga aaacatccag atgtccaacc tgcacgaagg
    37261 tcagagcttc ttcgaaatgc tgggtgaata cattctggca ggtttcaaag ttgccattat
    37321 cgttgccgcg atgctgatcg gctttatcgc cctgatcgcc gcgctgaacg cactgtttgc
    37381 caccgttact ggctggtttg gctacagcat ctccttccag ggcatcctgg gctacatctt
    37441 ctatccgatt gcatgggtga tgggtgttcc ttccagtgaa gcactgcaag tgggcagtat
    37501 catggcgacc aaactggttt ccaacgagtt cgttgcgatg atggatctgc agaaaattgc
    37561 ttccacgctc tctccgcgtg ctgaaggcat catctctgtg ttcctagttt ccttcgctaa
    37621 cttctcttca atcgggatta tcgcaggtgc agttaaaggc ctgaatgaag agcaaggtaa
    37681 cgtggtttct cgcttcggtc tgaaactggt ttacggctct accctggtga gtgtgctgtc
    37741 tgcgtcaatc gcagcactgg tgctgtaaaa ccatacataa aaaagccggg gatcattccc
    37801 cggctttttt acgcctgtta atcagctaat ggctgcgggc gaccaatcaa atacccttgc
    37861 agatattgca ccccgagctt atgcaatagc gcctgctgct gttgcgtctc gacaaactcc
    37921 gcgaccacac tcaatgactt cgctttcgcc agatcggtaa ttgatctcac aatcatcgca
    37981 tccagcgtat tcgtgacaat atctttcaca aagacgccat caattttgat gatatcagcc
    38041 tgcaaacgct ttaaccgttc gtagttggca tatccggtgc caaagtcatc aatcgcaatc
    38101 cggaaaccaa acttatgcag ctgctcgatg ttgtacatgc tgctttctgc gttagaaaac
    38161 gcctgctcct cggtgatctc aagaatgacc gcctgcgggg agatgtgata acgcttaaac
    38221 agacgaataa tccgcccggc aatattcttt tgcagcagcg tgagcggcat taaattgact
    38281 gaaaagcgcg gtcctttttt gtcgcaaggg tgtgttgcca gccacttcaa caaggattcc
    38341 agcacttgca aatcaaaacg cgcgctaagg ttaaactgag caataagggg cagaaactta
    38401 tccggggtca taatgccgcc gtcatatttc agtcgcgcga ggatctcatc ataaccttca
    38461 ccctctttgt tgcgaattgg ctgggcgtag agcagcaaat caccctgatc taacgccgtg
    38521 cgaatggtat tcagcaatag cacctgttta gtggtctgcc cggaaaccat ctcctctctg
    38581 ctatccagcg ccagcacatg atgatgtgcg caggattgct ccgccagcca gcttaactgc
    38641 cccaacaagg gttgcagggt ttcctgattt ccatcaaaac gcccccaggc agcaccatag
    38701 cccatatcca gcccggtatt gttccagtga atttgccgac tattcaggat gttaaccata
    38761 tgctggagtc gcccttccgt ttccggcccg ctcagcacta acagcagttc actacccggc
    38821 aattgataca acttttcgtt ttcctgcatc aacggcagca gcgtacggca aattgagcgg
    38881 atacagtgaa cgcgcatcat taagccgtaa tgacgactca taaactcaag attatcaatg
    38941 cgcaggcagc aaaaactctt gcccgcctct tgctccggcg cttgttccaa cgcacgaaag
    39001 ttgggtagga gcgttaacgg atctgttagc gcctgcaaat gccagcggcg attaagccat
    39061 tcactgcgat gataaatgcg caccatatag agcaggcaaa cgctaaagga aatcagcacc
    39121 gcgagaataa atgccagcga atattcggtt tccacccctt gcaaaaagtt ctggttgtaa
    39181 ttcagaaggc aaagcgttga aacagcccag gtgagattta aaaacggata gcgaagatta
    39241 ccgaccccga gggtaaagat gatgaagaaa acgggtacca ggtaaccggc aataaagtcg
    39301 ttttcataag gtgtgcacaa cagaagtagc agcacgctta acgctgccag ccagcttaag
    39361 gtaaatgcga gtgagatagt agaaaagcat gttgtaaatc agcactgcgg tgaaaaggct
    39421 tagtaaatca acgaccgtga aaatggcatc cgcatcgccg aaaaaggtgg atatctttag
    39481 cgggaaatca aagaagctcc ccacaagata catgctgcat ttgatgccga tcggcgtcac
    39541 caaaccaagc cagaacagac gttgccagac atatcggctg gtcaatccat aacgccaacg
    39601 cgtccccagc tgccagcgta atattgcgca ggcacacaac acgacaaacg tctggctgcc
    39661 gagcatgacc gcagtctgta aaaaagagag atgaaagttc cacagattgg tgcaaaacat
    39721 cccgcataaa acgggaacga caccgcgcca gccaaagata aacaagattg acagcattac
    39781 gcacagtggc atccacgcca gaaagatata actggaatgg ataatggcaa gtggcgaaat
    39841 aaaacgggat agctgaataa gtaccacggt gagcgtaaac gctagtgtga atatcttgat
    39901 attttttatc aggttatgct ccacaaacat gagtacacta ctctcactga tcggggtttt
    39961 caggcacaca aataataagt ttattcgcat cgcgaagcaa acaggggcca gcctgttgcc
    40021 gatttttcat ccaatgatgc tttcaccagg cggcgctaac ccggacgtag actgtattac
    40081 aaaagcggca aaaagcagag acaaaaaacc cccgctttgc agcgagggtt ggaaatttgg
    40141 tggagctaag cgggatcgaa ccgctgacct cttgcatgcc atgcaagcgc tctcccagct
    40201 gagctatagc cccacgatgc gtttacgtac caaatttgct gggtgcaaaa tttggtggag
    40261 ctaagcggga tcgaaccgct gacctcttgc atgccatgca agcgctctcc cagctgagct
    40321 atagccccgt cacgtaaagc ttgtcgagtt gacgggcggc atcatatgaa ttccgcccga
    40381 atgtgtcaac ggcaaattgc aacgtgtagt ttcaatcgct gaaaaatcag gcaaatgaac
    40441 aattttggaa aaccgctcgc attcagtagt tattcatgtc acggtttcct gtaaagtggt
    40501 gttataaaat gaactactaa tagacccaca tacattcagg gaattgttat gttcaaggag
    40561 aggatgacac cagatgaact tgccagactg accggctata gccgccagac cattaataaa
    40621 tgggtacgca aggaaggctg gacaacgtca ccaaaacctg gcgtccaggg tggcaaagcc
    40681 cgactggttc acgtcaatga acaggttcgt gaatatattc gcaatgctga acgtccagaa
    40741 ggtcagggag aagcgcctgc cctttctggt gatgcgccgc ttgaagtatt gctggtgaca
    40801 ctggcgaaag agatgacgcc agttgaacaa aaacagttta catccttgct tctgcgggaa
    40861 gggattatcg gattgttaca acgcttaggg attcgcgata gcaaataata tgaaaagatt
    40921 acgcaataaa atgaccaccg aagaactggc tgaatgcctc ggtgtggcta aacaaactgt
    40981 taaccgttgg accagagaaa aaggctggaa aacggaaaaa tttcccggcg tgaaaggcgg
    41041 tcgtgccaga cttattctgg tcgatacgca agtttgcgag tttattcaga acacgccagc
    41101 cttccataac acaccaatgt tgatggaagc tgaagagaga attgctgaat acgctcctgg
    41161 agcacgcgcc ccggcgtatc gacaaatcat caacgcgata gataatatga cagacattga
    41221 gcaggaaaaa gtcgcgcaat ttttgtcacg tgaaggaatt cgcaatttcc tcgcccgtct
    41281 cgatattgac gaatcagcat aaataaaaaa cggcaggata ttatctcctg ccgtttatct
    41341 ttttacacgc taattactgc tgattttcgc gttcagcaat aaaatccagc gctttgttga
    41401 tacgctcgat actgcgggtc ttaccaattg cgtgaacggt aacatccagt gctggagact
    41461 gccccgcacc ggttacggcg acacgcagcg gcataccaac tttacccata cccacttcca
    41521 gctcatccgc cgtcgcctga atagcgtgat gaacgttttc agcggtccag tcagtaatcg
    41581 cggccagttt gtcacgaacc acttccagcg gctgacgcgc taccggacgc agatgttttt
    41641 tcgccgcatc ggcatcgaac tcagcaaaat cttcgtagaa ataacggcag ctctgtgcca
    41701 tctctttcag cgtcttgcag cgttcgccca gcagtttcac cagatcagcc agctgcgggc
    41761 cgttacgggt atcgatattt tcctgctcaa tgtgccactg taagtgagta gcaacatact
    41821 ccggcggcag cgcgttaatg tagtgatggt tcagccacag cagcttgtcg gtgttgaacg
    41881 cactggcaga tttgctgacg gcattcaaag tgaagtattt gatcatctct tcacgagtga
    41941 agatttcctg atcgccgtgg gaccagccca gacgcaccag atagttcagc agtgcttctg
    42001 gcaaataacc gtcatcacga tactgcatta cgctgactgc cccgtgacgt ttggacagtt
    42061 ttttaccgtc atcgccattg atcatagaaa cgtgcgcgta aaccggcacc ggcgctttca
    42121 gggccttaag aatgttgatc tggcgcggcg tgttgttgat atggtcttcg ccacggataa
    42181 cgtgggtgat ttccatatcc cagtcatcga caaccacaca gaagttatag gttggggaac
    42241 catcggtacg gcggataata agatcgtcca gttcctggtt gctgaactcg atcggaccac
    42301 ggatctgatc gtcaaaaaca acagaacctt cctgcgggtt agcaaaacgt acaacacacg
    42361 gttcatcatc agcatgatgc tcatggctgt ggcggcagcg accgtcataa cgcggcttct
    42421 cacctttcgc catttgctct tcgcgcagcg cttccaggcg ctctttagag caatagcatt
    42481 tataagcagt gccctcttcc agcatctgat cgatcaccgc gttgtagcga tcaaaacgtt
    42541 tggtctggta gtacggacct tcatcccact ccaggctcag ccagttcatg ccatccataa
    42601 tggcttcgat agcttccggc gtggaacgct caagatcggt gtcttcaata cgcagcacga
    42661 actcaccgcc gtggttacgt gcaaaaagcc aggagtaaag agcagtacgc gcgccgccaa
    42721 cgtgcagata gcctgttggg cttggcgcga agcgagtttt gattttcatg aaatggcctt
    42781 acgtttagaa agatgccgac aaccggcaaa tcctggaaaa attgagtggg cgatattcta
    42841 tcactcccgc ctgattcctc aatgtagttc gggtttctac cttacgcatt attgttttcg
    42901 ttgacaaatt gcgcaacacg gtctgttttg cgatcatctt gcttaaattt acgacgaacg
    42961 aacaatttct ttagaaaatg cgttgactca ttttgaactc tccctataat gcgactccac
    43021 acagcggggg tgattagctc agctgggaga gcacctccct tacaaggagg gggtcggcgg
    43081 ttcgatcccg tcatcaccca ccaactactt tatgtagtct ccgccgtgta gcaagaaatt
    43141 gagaagtggg tgattagctc agctgggaga gcacctccct tacaaggagg gggtcggcgg
    43201 ttcgataccg tcatcaccca ccacttcggg tcgttagctc agttggtaga gcagttgact
    43261 tttaatcaat tggtcgcagg ttcgaatcct gcacgaccca ccaatgtaaa aaagcgccct
    43321 aaaggcgctt ttttgctatc tgcgatactc aaagattcga acctgcagca ggtttgagtt
    43381 gagcgcagcg aaacaacgga gccgctcgcg gcgacggccc gaagggcgag cgaagcgagt
    43441 catcctgcac gacccaccaa tgcaaaaaag cgccctaaag gcgctttttt gctatctgcg
    43501 atactcaaag attcgaacct gcagcaggtt tgagttgagc gcagcgaaac aacggagccg
    43561 ctcgcggcga cggcccgaaa ggcgagcgaa gcgagtcatc ctgcacgacc caccaatgca
    43621 aaaaagcgcc ctaaaggcgc ttttttgcta tctgcgattt gtaagattga tttttcttct
    43681 caagttcctt gttcttctac cgatattacc tcgtacagtg acgaggactg aacaatggcc
    43741 actatctctt caacttcaat tccttccata caaacacaga gtagtaacag ggcatcacaa
    43801 ggtagcgatg ttgcttcaca aatcgcgcgg ataagtcagc aaatcatcaa actcactcaa
    43861 caaattaaag agattgtaga cactagcggc agtgctgaag acaagcaaaa acaggccgaa
    43921 ttgattcaac agcaaatcac cctactggaa acacaactgg cgcagttgca gaaacagcag
    43981 gcagaaaagg cgcaggagaa agagcagcgt ttgtctctga atgtatccct tctaaatcct
    44041 gttgaaaaaa cgactcatat cgatatctat atctgataca acggatcccc ttcccgacca
    44101 gccattagcg cattgccctg gtctcgtacc acgtcccata acgcttcagc ggcggtggaa
    44161 agtgaacggt tcttcctgcg caccagcatc aactggcgtt caaccaccgg cgtaatgcgc
    44221 ttaaccacca gtgggctacc ttcaggcaat ggtagcgcca gtgcaggaag aatactgatg
    44281 ccgatgcctg cggctaccat cgggaacagc gtcgccggat gtccaatctc ctgcacaata
    44341 ttcgcctgaa taccattgcg cgccagcgct gcgtcaatca gcggtcggct gcctgacgcg
    44401 taatcctgca acaccaattt cgctccctgt aatgcctgcc agggtacata atcttccacg
    44461 gccaaagcgc tatcgcgatg gcaaagcaga aagaaaggct cggaaagaat cgcttcacat
    44521 tgcagatcgc ccactgggcc tggatcgatg acgatgccaa agtcgacatc tccctggcga
    44581 atactttcca tcacccactg ctgcggacga tcatgcagga caaactgaat atctggatag
    44641 cggcgatggc tttcagcaat gcattgcgga ataagatgcg cggaaatggt ctggctggca
    44701 gcgacccgaa cttttccgct cagttgttgc cccatacgcc cggtatcgcg caacgtgctg
    44761 ttaagttcat ccagcagtcg ctcaagacgc aaagccaact gctgccctgc atctgtaagc
    44821 accacttcac gcgtggttct gtccagcagg cgaacaccag tatgattttc cagctccttc
    44881 acactgtgac ttactgccga ctggctcaag ccgatacgct ctcctgcacg actaaaactt
    44941 ttctcctgcg ctactgtgac gaaaaccttt aattgtttta aagaataatt catctgtttt
    45001 cttcatgaat ggatgcaata aatcaatttt atttctcaaa ctggaagaag cacaatagaa
    45061 ccatcgatca tctggagtct ttatgaaact ttttcgtatc ctcgatcctt tcaccttaac
    45121 cctgatcacg gtggtgttgc tggcctcttt ctttccggcc agaggcgatt tcgtcccctt
    45181 ctttgaaaat ctgaccaccg cagctattgc cctgctgttc tttatgcacg gcgcgaagtt
    45241 gtcgcgtgag gcgattattg ctggcggtgg tcactggcga ctgcatttgt gggtaatgtg
    45301 cagcaccttc gtgctgtttc cgattctggg tgtactgttt gcctggtgga aaccggtaaa
    45361 tgtcgacccg atgctctact ccggttttct ctacttgtgc attctcccgg ctaccgtgca
    45421 gtctgcaatc gccttcacgt caatggcggg cggtaacgtc gcggcggcgg tttgttctgc
    45481 gtcagcatcc agcctgctgg ggattttcct ttcaccattg ctggttggtc tggtgatgaa
    45541 tgttcacggt gcaaggggca gccttgagca ggtcggtaaa attatgctgc aactgctgct
    45601 gccgtttgtg ttggggcatc tttcccggcc gtggattggt gactgggtgt cgcgcaataa
    45661 aaaatggatt gcgaaaactg accagacgtc cattctgttg gtggtttata cagcgttcag
    45721 cgaagccgtc gttaatggta tctggcataa agttggctgg ggatcattgc tgtttatcgt
    45781 ggtggtcagc tgcgttcttc tggctatcgt gattgtagtt aacgtcttta tggcacgccg
    45841 actgagcttc aataaggcag atgaaattac tatcgtcttt tgtggttcga aaaagagtct
    45901 ggcaaatggc atcccgatgg caaacattct gttccccaca tcggtgatcg gtatgatggt
    45961 gctgcccctg atgattttcc atcagatcca attgatggtc tgtgcggtgc tggcgcgtcg
    46021 atacaaacgc cagaccgaac agttacaggc gcagcaggaa agcagcgccg ataaagctta
    46081 aagcggacgc ttcaggggct ggaccagttg cgtcagcccc tcggttttaa tcagcagcgt
    46141 gatttgcatt agctcaccga gcttccctgc cgggaactca tctttgcggg caaaccacag
    46201 cagatactcc tccggcaggt caattaagcg acgccctttg tatttgccaa acggcattat
    46261 cgtattggct atttcaatca gctgctcttt ttccatctca gctacccagc aaacgcagca
    46321 tttccgcttc gtcgatgact tcaatgccca gttcctgcgc cttcgccagt ttagatcctg
    46381 cagcttcacc cgctatcacc agatcggttt tcttcgacac gctgcccgcg actttcgcgc
    46441 ccagttcgac cagtcgagct ttagcgtcat cacgcgacat ctggcttaag ctgcccgtaa
    46501 gcaccacggt tttaccagca aacgggctgt caatctcttc cgcgttgata acgatcggcg
    46561 caggccagtg aacaccttcc gccaacagct cgctgatgac attgcggttg ctttcttcgg
    46621 caaagaagtt gtgaacgtgg gatgcaacga caatgccaac atcaggcacc ttttgcagct
    46681 cttcaatcga agcggcttcc agcgcttcca gcgtgccgaa atatgccgcc agacctgctg
    46741 cggtggcctc gccgacttca cggatgccaa gtgcatagag gaagcgagca aaggtggttt
    46801 ctttcgcttt ttccagcgcg ttaaccacgt tttgtgccaa ttttggcccc atacgctcca
    46861 gtccggtcag tttgcctgcg gtgagtttga acagatctgc cggagtatgg acatattctt
    46921 tttcaaccag ctgatcgatg attttgtcgc ccattccgtc aacatccatc gcacggcggg
    46981 aaacaaagtg tttcagcgac tctttacgct gcgcaccgca aatcaggcca ccggtacagc
    47041 gggcaaccgc ttcaccttcc acacgctcaa cgtcagaacc acataccgga caatgcgtcg
    47101 ggaatacaac ctcacgggta tcttccgggc gttcagaaag cacgacgtta accacctgcg
    47161 ggatcacgtc gccagcgcgg cgaatcacca ctttatcgcc aatgcgtaaa ccaagacgtt
    47221 cgatttcatc cgcattgtgt aaggttgcgt tactcaccag cacgccagca acatggacag
    47281 gttccagacg cgcaacaggc gtaatcgcgc cagtacgccc aacctgaaac tcgacgtcac
    47341 gcacaaaggt catctgctcc tgcgccggga atttaaacgc aaccgcccag cgcggggcac
    47401 gcgcgacaaa gccaagctgc tcctgctgtt ccagtgagtt gaccttaatc accacgccgt
    47461 cgatatcaaa gcccagcgtc gggcggtctt cttccacttt gtgatagaac gccagcactt
    47521 cttccgccga ttcacaaagc gttacccgat cgctgaccgg caccccccac tttttaaatt
    47581 gcagtaaacg gccaagatga gtatccggca gctcgccacc ttccagaaca ccaacgccat
    47641 agcagaaaaa agtgagcggt cgcttcgctg taatacgcgg atcaagctga cgcagtgaac
    47701 cagctgccgc attacgtggg ttagcaaaaa ctttcccgcc cgtgcgtcgc gcatcttcgt
    47761 taatcttttc gaaccccgcc tgcggcagga acacttcacc acgcacttcc agacgcgccg
    47821 ggatattctc tccgtgcagc ttcagcggaa tggcgcgaat agtacgcaca ttagacgtga
    47881 tatcttcccc ggtggtgcca tcgccacggg tcgcggcact gactaaaacg ccattttcat
    47941 acagaatact gacggcaagg ccatccagct tcagctcaca gcaccaggtg actttctcgt
    48001 tgtttttcag acggtcctgc acacgtttgt tgaaagcaag aaagctttct tcatcaaaaa
    48061 cgttatccag tgacagcatt ggtacttcat ggcgtatctg gctgaaagct gccagcggcg
    48121 cagcacctac acgttgggta ggcgaatcag gcgtaatcag ttctggatgt ttggtttcca
    48181 gctcgcgcag ttcgcgcatc agcctgtcgt attcagcgtc gggaatttcc ggcgcatcca
    48241 tcacatgata aagatattca tgatggcaaa gcgtcgttcg cagttctgtc agttgttgtt
    48301 cgattgattc catatcgcac catcaatgct aaaaaccccc gacaagcggg ggttcgaaga
    48361 ggagttaatt tgccttaagt gtatcaggcg ttggcgtctt tgacttcgcg gatgatgtcc
    48421 tggtactcgc gcaatttctg cggagtcatc atacggcgct gatcgtcaag cacgacaccg
    48481 cccacttcat cggcaatatg ctgcgcagat tgcagcatca gcttgaagtt ctgcagctcg
    48541 tcaccgtaag acggtacctg cataaagata gtgacacccg gagtagtgaa atccttcatt
    48601 tcaggatcaa aggttcccgg tttcaccata ttcgccaggc tgaataacgc cgggccgctg
    48661 ccatccgggc taagatgacg atggtaaata ttcatatcgc caaaaatgaa gcccgcttgt
    48721 tgaatgctgt taagaagcag ttcaccgttt agctcgctac cgtgatgcgc cgcgacgttc
    48781 atgataatca ccgcttcttt gcgcttcggt ttatccataa ctggagccgg ttccgctaca
    48841 ggctcaggct gtggtgccgc tacgggttct gcaggctgga aagcctgttg tgccggttgc
    48901 ggtgctgaat gcacaggctg cggcgctggc gcgacctgtg gcgaaactgg ctgctgcaac
    48961 ggctgttcag gctgcggctg ataggcaggc tgctgcaccg gctgcgctgg acgcggagca
    49021 tgttgcggcg gtacctgcgc ttcaggcggc tgctggaccg gttgacgcgg ctgcgcagac
    49081 gcataaggcg gtaggtactg gtgttgcggc gacggacgag cagcctcatg ctcctgagcg
    49141 ttagccgggg catgattcac gcggtgaaca cgaacctcac caacgccctc atcatcttcg
    49201 acatcctcgt cataagaatc gtcgtcacgt tttgacttca ttcgttttaa tggccgatcg
    49261 cggaacatag aagatcgttc tttacggctg gtccagaaac catgtaccag taaagcgatt
    49321 atggcgatcg cgccaacaat gattaatatc agacgcaaat cctgcatcat tatattctct
    49381 gttgttctaa caccttgcca ccacggcaaa catttactca ctaagagtat ttgccgatta
    49441 cctcaagtgc aagtgcacta ttaactttca cagcacaaag atagatgaaa tcgtgctttt
    49501 tgctgttttt tcgaacatat cctaactgtc cattgcgcaa ttacccggta aaatacgcag
    49561 aattttcctg ggattggtca aaaggagctc atcctgatta tggtttcatc attcacatct
    49621 gccccacgca gcggttttta ctattttgcg caaggctgga agctcgtctc gcaacctggg
    49681 attcggcgtt tcgttatttt accgctgctg gtcaatattt tgttgatggg gggcgcattc
    49741 tggtggctct ttacacagct cgatgtctgg atcccgactt tcatgagtta cgttccggac
    49801 tggctacaat ggctgagtta tctgttgtgg cctctggctg tcatctctgt gctgttagtg
    49861 tttggctatt tcttctccac gattgctaac tggattgccg ctccgtttaa cggtttattg
    49921 gctgaacaac tggaagcacg attgactggc gctacaccgc cagataccgg gattttcggt
    49981 atcatgaaag atgtgccgcg aatcatgaaa cgcgaatggc aaaaatttgt ctggtatctg
    50041 ccgcgcgcaa ttgtattact aattctttac ttcatccctg gtattgggca aaccgtcgcg
    50101 ccggtactgt ggttcctgtt tagcgcctgg atgttagcca tccagtattg cgattacccc
    50161 ttcgataacc acaaagtgcc gtttaaagag atgcgcaccg ccctgcgcac acgcaaaatc
    50221 accaatatgc agtttggtgc tttaaccagc ctgtttacga tgatcccgct gcttaatctg
    50281 ttcatcatgc ccgttgccgt ttgtggcgcg acggcgatgt gggtcgattg ctatcgcgat
    50341 aaacacgcga tgtggcggta acaatctacc ggttattttg taaaccgttt gtgtgaaaca
    50401 ggggtggctt atgccgcccc ttattccatc ttgcatgtca ttatttccct tctgtatata
    50461 gatatgctaa atccttactt ccgcatattc tctgagcggg tatgctacct gttgtatccc
    50521 aatttcatac agttaaggac aggccatgag taagattttt gaagataact cgctgactat
    50581 cggtcacacg ccgctggttc gcctgaatcg catcggtaac ggacgcattc tggcgaaggt
    50641 ggaatctcgt aaccccagct tcagcgttaa gtgccgtatc ggtgccaaca tgatttggga
    50701 tgccgaaaag cgcggcgtgc tgaaaccagg cgttgaactg gttgaaccga ccagcggtaa
    50761 taccgggatt gcactggcct atgtagctgc cgctcgcggt tacaaactca ccctgaccat
    50821 gccagaaacc atgagtattg aacgccgcaa gctgctgaaa gcgttaggtg caaacctggt
    50881 gctgacggaa ggtgctaaag gcatgaaagg cgcaatccaa aaagcagaag aaattgtcgc
    50941 cagcaatcca gagaaatacc tgctgctgca acaattcagc aatccggcaa accctgaaat
    51001 tcacgaaaaa accaccggtc cggaaatctg ggaagatacc gacggtcagg ttgatgtatt
    51061 tattgctggc gttgggactg gcggtacgct gactggcgtc agccgctaca ttaaaggcac
    51121 caaaggcaag accgatctta tctctgtcgc cgttgagcca accgattctc cagttatcgc
    51181 ccaggtgctg gcaggtgaag agattaaacc tggcccgcat aaaattcagg gtattggcgc
    51241 tggttttatc ccggctaacc tcgatctcaa gctggtcgat aaagtcattg gcatcaccaa
    51301 tgaagaagcc atttctaccg cccgtcgtct aatggaagaa gaaggtattc ttgcaggtat
    51361 ctcttctgga gcagctgttg ccgcagcgtt gaaactacaa gaagatgaaa gctttaccaa
    51421 caagaatatt gtggttattc taccatcatc gggtgagcgt tatttaagca ccgcattgtt
    51481 tgccgatctc ttcactgaga aagaattgca acagtaatgc cagcttgtta aaaatgcgta
    51541 aaaaagcacc tttttaggtg cttttttgtg gcctgcttca aactttcgcc cctcctggca
    51601 ttgattcagc ctgtcggaac tggtatttaa ccagactaat tattttgatg cgcgaaatta
    51661 atcgttacag gaaaagccaa agctgaatcg attttatgat ttggttcaat tcttccttta
    51721 gcggcataat gcttaatgac gtacgaaacg tcagcggtca acacccgcca gcaatggact
    51781 gtattgcgct cttcgtgcgt cgcgtctgtt aaaaactggc gctaacaata caggctaaag
    51841 tcgaaccgcc aggctagact ttagttccac aacactaaac ctataagttg gggaaataca
    51901 atgttccagc aagaagttac cattaccgct ccgaacggtc tgcacacccg ccctgctgcc
    51961 cagtttgtaa aagaagctaa gggcttcact tctgaaatta ctgtgacttc caacggcaaa
    52021 agcgccagcg cgaaaagcct gtttaaactg cagactctgg gcctgactca aggtaccgtt
    52081 gtgactatct ccgcagaagg cgaagacgag cagaaagcgg ttgaacatct ggttaaactg
    52141 atggcggaac tcgagtaatt tcccgggttc ttttaaaaat cagtcacaag taaggtaggg
    52201 ttatgatttc aggcatttta gcatccccgg gtatcgcttt cggtaaagct ctgcttctga
    52261 aagaagacga aattgtcatt gaccggaaaa aaatttctgc cgaccaggtt gatcaggaag
    52321 ttgaacgttt tctgagcggt cgtgccaagg catcagccca gctggaaacg atcaaaacga
    52381 aagctggtga aacgttcggt gaagaaaaag aagccatctt tgaagggcat attatgctgc
    52441 tcgaagatga ggagctggag caggaaatca tagccctgat taaagataaa cacatgacag
    52501 ctgacgcagc tgctcatgaa gttatcgaag gtcaggcttc tgccctggaa gagctggatg
    52561 atgaatacct gaaagaacgt gcggctgacg tacgtgatat cggtaagcgc ctgctgcgca
    52621 acatcctggg cctgaagatt atcgacctga gcgccattca ggatgaagtc attctggttg
    52681 ccgctgacct gacgccgtcc gaaaccgcac agctgaacct gaagaaggtg ctgggtttca
    52741 tcaccgacgc gggtggccgt acttcccaca cctctatcat ggcgcgttct ctggaactgc
    52801 ctgctatcgt gggtaccggt agcgtcacct ctcaggtgaa aaatgacgac tatctgattc
    52861 tggatgccgt aaataatcag gtttacgtca atccaaccaa cgaagttatt gataaaatgc
    52921 gcgctgttca ggagcaagtg gcttctgaaa aagcagagct tgctaaactg aaagatctgc
    52981 cagctattac gctggacggt caccaggtag aagtatgcgc taacattggt acggttcgtg
    53041 acgttgaagg tgcagagcgt aacggcgctg aaggcgttgg tctgtatcgt actgagttcc
    53101 tgttcatgga ccgcgacgcg ctgcccactg aagaagaaca gtttgctgct tacaaagcag
    53161 tggctgaagc gtgtggctcg caggcggtta tcgttcgtac catggacatc ggcggcgaca
    53221 aagagctgcc atacatgaac ttcccgaaag aagagaaccc gttcctcggc tggcgcgcta
    53281 tccgtatcgc gatggatcgt aaagagatcc tgcgcgatca gctccgcgct atcctgcgtg
    53341 cctcggcttt cggtaaattg cgcattatgt tcccgatgat catctctgtt gaagaagtgc
    53401 gtgcactgcg caaagagatc gaaatctaca aacaggaact gcgcgacgaa ggtaaagcgt
    53461 ttgacgagtc aattgaaatc ggcgtaatgg tggaaacacc ggctgccgca acaattgcac
    53521 gtcatttagc caaagaagtt gatttcttta gtatcggcac caatgattta acgcagtaca
    53581 ctctggcagt tgaccgtggt aatgatatga tttcacacct ttaccagcca atgtcaccgt
    53641 ccgtgctgaa cttgatcaag caagttattg atgcttctca tgctgaaggc aaatggactg
    53701 gcatgtgtgg tgagcttgct ggcgatgaac gtgctacact tctgttgctg gggatgggtc
    53761 tggacgaatt ctctatgagc gccatttcta tcccgcgcat taagaagatt atccgtaaca
    53821 cgaacttcga agatgcgaag gtgttagcag agcaggctct tgctcaaccg acaacggacg
    53881 agttaatgac gctggttaac aagttcattg aagaaaaaac aatctgctaa tccacgagat
    53941 gcggcccaat ttactgctta ggagaagatc atgggtttgt tcgataaact gaaatctctg
    54001 gtttccgacg acaaaaagga taccggaact attgagatca ttgctccgct ctctggcgag
    54061 atcgtcaata tcgaagacgt gccggatgtc gtttttgcgg aaaaaatcgt tggtgatggt
    54121 attgctatca aaccaacggg taacaaaatg gtcgcgcctg tagacggtac cattggtaaa
    54181 atctttgaaa ccaatcacgc gttctctatc gaatctgata gcggcgttga actgttcgtc
    54241 cacttcggta tcgacaccgt tgaactgaaa ggcgaaggct tcaagcgtat tgctgaagaa
    54301 ggtcagcgcg tgaaagttgg cgatactgtc attgaatttg atctgccgct gctggaagag
    54361 aaagccaagt ctaccctgac tccggttgtt atctccaaca tggacgaaat caaagaactg
    54421 atcaaactgt ccggtagcgt aaccgtgggt gaaaccccgg ttatccgcat caagaagtaa
    54481 ttcttgccgc agtgaaaaat ggcgcccatc ggcgccattt tttatgcttc cgccagcggc
    54541 ggcaaaatca attcatcgct ctcatgctgc tgggtgtagc gcatcacttc cagtacgcgc
    54601 aaccccgcac ggtgcaccgc atcgtttaac gccttccctt tcagcaaacc actgataagc
    54661 tgagcacaaa acaggtcgcc agtccctttc aggtcggttt ttacccgtga atgggaaatg
    54721 acattcacgc tgtcggcact gaccaccacc acctgcatct cctgattttc ttcattaccg
    54781 gaggcgctgg taatcaccac ccattttaat gtgtctgaaa gcagactttt tgcggcagca
    54841 atggcactgt cgagatcgcg gcaattttta ccggtcagga tttccagctc aaagatattg
    54901 ggggtaattc cctgcgccag cggcagtaaa tattgtcgat acgcctcggg aaggtcaggt
    54961 ttgacataaa ttccgctatc aatatcgcca atcaccggat cgaccatgat caataggtca
    55021 ggatggtctt tgcgtagcgc agtcagccac tcggcaagga ttttgatttg cgatgccgtt
    55081 cccatatagc ccgtggttac agcacgaagt tggcgcagcg catcacgctc ctgaagcgca
    55141 cgcaaatagc cgctaaacca ttcgtccgga atcgcaccac cgtagaaagt gtcataatgc
    55201 ggcgtattgc tcagcaatac cgtcggcacg gcaaagacat tcaagccgtt ctgttttata
    55261 gcaggcaccg caatgctgtt gcctacgctt ccgtacacta cctgcgactg cacggcgacg
    55321 atatccgcct gcagcgccct actcttatcg ttaaacaaca acaaactact catttaattt
    55381 tttctccttg ccgatgatcc tcatcgtatt ccaaccgaaa ctttacctga ttctggcagt
    55441 caaatcggct attacaaaac aaagataagg taattcagtg aagaaaatca tttgtctggc
    55501 cattacgctg ctaatgacac tccccgctta cgcgaagtta actgttcatg aagaagcccg
    55561 catcaacgcc atgctggagg gattagcaca gaaaaaggat ttgatatttg tgcgcaacgg
    55621 tgatgaacat acctgcgatg aagcggtttc ccacctgcgt ctgaagctcg gcaatacccg
    55681 taaccgcatt gacactgccg agcagtttat tgataaggta gcttcgtcgt catcgattac
    55741 cgggaagccg tatatcgtga agatccccgg taagagcgat gagaacgcac agcctttttt
    55801 acatgcgtta attgcgcaga cggataaaac ggtgcctgcg gaaggaaatt aatccgcttt
    55861 ggtaaggcat ttacaggagg taacatgaaa aaatgcttta tttatcacga tgaaaaatcg
    55921 aataaatttt ggtggataga ttacgaaggg gatagtttag ctgtcaacta tggcaaggta
    55981 ggtagtattg gtaaattcca tacaaaagaa ttcgataatg aagaacagtg tctgaaagaa
    56041 gccagtaaat tgattgccgc aaaaatgaaa aaaggatatc aagaaactcc agactttaac
    56101 ttcatggatc tctactattt tgatgatgaa gagattgggt tacatcttaa aacgtcacac
    56161 ccaaacttcc agtgccattt tactgatcca ctttatatgt gttgctggga tgaagaatct
    56221 ccttttggta gcgatgaagg tgctgatgct ctaaacgaac ttgaaaatag cctccgttct
    56281 ggactgtgct gatttccctc aaatgttaat tgaaactatg tggggtatga aatacatcgc
    56341 tatggacagt attcttgaag aggatgtgcg tgcgcaatta ctagccgatg aaatgagctc
    56401 tatccagagc aatatgatta cctacgcgac tgcattcggt cagatcaaag tcatgggtaa
    56461 aatctcccat aaacttaaaa agatgggact caatgcacta gcgcgtcatc agcttaccgc
    56521 aaaaattctt caatggggtg acggtcagga ctcaccaata cttcaaaaaa tgattgatga
    56581 ccttactgct tttccacacg aaaattaaaa actgcatttg tcggcagcaa caactgccga
    56641 caaatcagat tattgacaaa gtgcactttg tttatgccgg atgcggcgta aacgcctgat
    56701 ccggcataaa aatattgcta attcaatata ttgcagaaac attgtaggcc tgataagcgg
    56761 agcgcatcag gcagttttgc gtttgtcatc agtctccaat gctattaatc cttaaatccc
    56821 tgccccctga ctaaaatgct cttccccaaa cactccggtg gagaggtagc gatcgccacg
    56881 atcgcagatg atcgctacca ccaccgcacc agggttagct tttgccaccc gcagtgctcc
    56941 agcaaccgcg ccgcctgagc tgacgccaca gaatattcct tcccgcaccg ccagttcgcg
    57001 catggtgttt tccgcatcgc gctgatgaat atccagcacc tcatccacca gcgcagcgtt
    57061 gaaaatcccc ggcagatatt ccgcaggcca gcggcgaatg cccggaatac tgctgccttc
    57121 ttccggttgc aggccaacaa tggtcaccgg tttggattgt tcgcgcataa agcatgaaac
    57181 gccggtgata gtgccggtcg tccccatgct ggagacaaaa tgagtgatgc gcccgccggt
    57241 ttgctgccag atttccggcc cggtggtggt gtaatgcgca taagggttat cgggattatt
    57301 gaactgatcg agcagctttc cttcgccacg attcgccatc tccagcgcca gatcgcgcgc
    57361 accttccatg ccctgctctt tggtgacaag aatcagttcc gcaccataag cacgcatcgc
    57421 cgcacggcgt tcctggctca tgttgtcggg catcagcaat ttcatgcgat agcctttcag
    57481 cgcggcaatc attgccagcg caatgccggt gttaccactg gtggcttcga ttaagacatc
    57541 acccggttta atttccccgc gcttttccgc ctcgacgatc atcgaaagtg ccgcacgatc
    57601 tttcaccgaa cctgccgggt tattgccttc cagttttaac cacacttcac tgccgttatc
    57661 cggccccatt cgctgcaact tcaccagagg cgtattgcct attgtttgtt ctaatgtact
    57721 cacaatctct atccatacgt ggtgttgcct gatgcgacgc ttgcgcgtct tatcaggcct
    57781 acaggttaca aaccttgcca ttaaaaagcc cggatcgcgg gaagcgcctc cgggcgttta
    57841 acattcactc aacctatcag gcgctttgtg cgagagcaag ttcctcatcg cgggtttcaa
    57901 tacgctcgtc gccgttatac agccgcgcat gttgcagacc aacgaataaa cgctcgccat
    57961 gctgcggggc atcgtcgcca tgcatcacga ccgtcagcgg ttcgttgtac caccccagcg
    58021 gctgcaccac taattgggtg tagtgacctt tcgggctggc ttccagtacc tgtaccggca
    58081 gcggcgaatc gagactggta cggcggctga tatccacttc ccacgggcgc aggaagagat
    58141 ccaccggccc ctgatacgca ggtgtgtagc ccagcggcca gcgatgcgcg ccaacatgga
    58201 actgcccgcc gcgaatggtt ccctgcaggc ggttcacttc gcccataaat tcgagtacaa
    58261 aacgggtcgc tggttcgcgc catacctgat tcggcgcatc agcctgttca atattcccct
    58321 gactcatcac cactacacga tcggctactt cggtcgcctc ttcctggtcg tgggtcacaa
    58381 acacgctggt gaatttcagc tcttcatgca gttgacgcag ccagcgacgc agctctttac
    58441 gcacctgcgc atccagcgcg ccgaacggtt catcaagcag cagaatttgc ggttctacag
    58501 ccagcgcgcg cgccagcgcc acacgctgtt tctggccgcc ggaaagctgc gccggataac
    58561 gatccgccag atgcgcaagc tggaccattt ccagcaattt tgtcactttc gctttgatgg
    58621 ctgcggcatt cgggcgctcg cgacgcggca gcaccgtcag gccaaaagtg atattgtcga
    58681 ataccgtcat atggcggaac agcgcgtaat gctggaacac gaaaccgact ttacgatcgc
    58741 gcgcgtgcag gcgactcacg tcggtgccgt ggaagcgaat atgcccgctg gtttgatgct
    58801 ccagcccggc gataatccgc agcagcgtgg ttttcccgga accggacggc cccagcaacg
    58861 cgaccatctg accagaagga atatccagtg agatatcgtt cagcacctgg gtgcgaccaa
    58921 aagacttctt gatattggca atctcaatgc tcatgatgct cctcctgctg cgcgcgtttt
    58981 tcctgattct ccaggcgcca ctgcaacata ctctttaaaa acagggtgat aatcgccatc
    59041 aacgtcaaca gcgcggcggc ggtaaaggag ccgacggtgt tgtagtcctg ctccaacaat
    59101 tcaatctgta acggcagcga aagtgtttcg ccgcgaatcg agccggaaac caccgacact
    59161 gcaccaaact cgccaatcgc gcgggcgttg gtcagcacca cgccgtaaag cagcgcccag
    59221 cgaatattcg gcaatgtgac gcgacggaac atctgccagc cggacgcgcc aagcaaaatc
    59281 gccgcttcgt cttcctggct gccctggctt aacatcaccg gcaccagctc gcgcaccaca
    59341 aacggacacg tcacaaagat ggtgaccagc accattccgg gccaggagaa cataatttgc
    59401 agattatgtt catccagcca gccgccgagc gggccgttgg agccgtagaa cagcagatac
    59461 accagaccgg caaccaccgg cgatacagca aacggaatgt ccagtagcgt cagcagtaac
    59521 tggcgtcccg ggaagttaaa gcgcgtcacc agccaggcca gcagaatgcc gaacaccagg
    59581 tttaccggta cggcaatcag cgcgatcatc accgtcagcc agatggcgtg cagcatgtcc
    59641 ggatcggcca gattctgtaa aaccggcatc agccccttgc tgaatgcctg cacgaagatg
    59701 taaatcatcg gcaccagcag gatgaacgcc gaaaccagca tcccgatgcc aatcagaaac
    59761 catttgcccc agttaatcgg gcgcgcgtca taacgcttca attgggtaac ttccgccatt
    59821 aatgacctac cacacgccga ccaaagcgac tttgcagagt gttaattgag aacagcagca
    59881 gcagagatgc cgcgaggatc accgaagcaa tcgcgctcgc tgccgggtaa tcaaactcct
    59941 gtaagcgcac aaaaatcatc agcgacgtca cttccgtctt ccacgcgata tttccggcga
    60001 taaaaatcac cgcgccaaat tcaccaagac tacgggtaaa cgacagcgcc acgcccgcca
    60061 ccagcgccgg agaaagctcc ggcagcacca ctttgcagaa actctgccag cgcgttgcac
    60121 caagcgtttc cgccgcttct tcatattccg ggcctaactc ttccagcacc ggctgcacgg
    60181 tacgcaccac aaacggaatg ctggtaaagg ccatcgccac cgcaatacca agccaggtat
    60241 aggtgacttt gatatcaaac ttcgccagcc attcaccgta aaaaccgttt acggaaaaga
    60301 gcgaggccag cgttaaaccg gcgactgccg ttggcagcgc aaagggtgaa tccatcagcg
    60361 catcaagcag cgtgcggccc ggaaagcgat aacgtgttaa aatccacgcc atcagcagac
    60421 cgaaaacgcc gttaaaaatc gatgccacaa acgccgacag cagcgtcact ttgtaggccg
    60481 cgaccacctg cgggttggtg atcacctccc agtactgcgc ccagctcatc tcagaaagct
    60541 gcatcaccag cgcagagagc ggcagcagca atatcaggca cacaaacagc agactggtgc
    60601 caaggcttaa agtaaagccc ggcagcacgc gtcttgaaga aactgcaaac attacttacg
    60661 ccccgccgct aacagcttgt ctaactcgcc gccgctggtg aagtgggttt tcatcacttc
    60721 cggccatgag ccaaatttgt cttccacgcg gaacagttcg gtctgcggga atttatcttt
    60781 cagtttgtcc atgacttccg ggttattcac acggtagtaa tagtcggtga tgatggtctg
    60841 cgcctgtggg ctgtagagcc agttcaggta ggcttttgcg gctttttccg taccgttggc
    60901 ctgcacattt ttatcgaccc acgccaccgg gaattccgcc agaatattgg ttttcgggat
    60961 caccacttca aagccctgtg cttcatactg tttacggatg ttgttcactt ccgattcaaa
    61021 gctaatcagc acatcgccca agccacgctc ggcaaaagtg gtggttgcgc cacgaccgcc
    61081 ggtatcgaac acttcaacgt ttttcaggaa ctgggtcata aactgttcgg ttttggcttt
    61141 atcgccaccg tcagctttat ccgctgcgcc ccatgccgcc agataggtat aacgcgcgtt
    61201 acccgacgtt ttcgggttcg ggaaaatcag cttcacgtcg gagcgcacca ggtcgttcca
    61261 gtcgtggata ttcttcgggt tgcccttacg caccaggaag cccatggtgg agtagaacgg
    61321 cgagctatta ttcggcaggc gcgactgcca gtcagccggg atcagcttgc ctttgtcgtg
    61381 caggatttgt acgtcggtca cctggttata agtgacaacg tctgctttta agccctgcaa
    61441 aatcgccagc gcctgttttg atgacccggc atgagattgt tttatcgtca gtttgtcgcc
    61501 gccgttatct tttgcccatt gctgttcaaa cggcggattc agggcggcaa acagctcgcg
    61561 ggagacgtca taagaactgt tcagcagttc cgttgcctgt acatggcccg ccagcagcag
    61621 agaagcgacc agcgcgagtg agttcttttt cagtaagtta acggccattg cgcaccctta
    61681 taaatttaat gactttctaa tagccatcat atttataacg gacgtgaaag gagtaacggt
    61741 tttatatacc gtttggtgat ttggaagtta aaaagggaat aagaacttgg ttcaacaggc
    61801 gtaattgttg caggcagttt ggacacggac agcgcggaga aaccggagcg tacacatagt
    61861 acgtgaggat ttcgagcact gcccggggcc aaaatgacaa atgaaatagc ctggtgaact
    61921 tagttcaaga cgaaatcctc cccacacatg cagggaggaa acagaggtga atcagatacc
    61981 gacgctaacc gtctccggca gtgtgctgcc gccatcaatc acattctgtg taccggttaa
    62041 atagctggat tcatccgatg cgaggaaggc cgccagttcg ccgacttcca gcggatcggc
    62101 gaggcgacac agcgggattg cttttgccat ttcagtcagc accgattctg gatcttccgg
    62161 gttcgactgg cgggcaatgc tttccgccat tggcgtgcgg acgtatcccg ggcagatggc
    62221 gttaacgcga ataccggatt gcgcgtactc caccgccagc gatttagtca ggccaacaat
    62281 cgccgctttc gttaaggcat acgccgtttc gccaggatcg gccaccatat caccagtgac
    62341 tgaagacatc atcacaatgc gaccatcttt gcgcgcaatc atctccggca gcaccgcctt
    62401 cgtgacgttc catacgcctt taatattgat atcaatatgg aaatcgcgat cttcatcgct
    62461 catatcgagg aaactgccca gacgacaaac gcctgcgtta ttcaccagga tatcaatgcg
    62521 cccttctttt tccttcgcgc gtttgatagc tgcggctacc gacgccgggt cacgcacatc
    62581 ggcgacaacc gccgtacagc gatgaccacg accacacagt tcgtccgcta gcttttcgat
    62641 ctcaggggag atatccagca agattaggtt cgcgccatga cgtgcaaaag ttctggcaat
    62701 tccttcgcca attccctgca atgcgcccgt aatcagtgct gtcttgcccg tgagtttacc
    62761 cattttaatg ctctccttgt taatacagag cgttacactc cattccctct gaaaatacag
    62821 gataaacaaa atattgctgc ggatcagctc actaattcag gaaaatctca atccagtcgc
    62881 tgctttatgc atcatttcag gcgttgtgtt agctcactgc tgcgctgaat cattttcaat
    62941 gacgccacat cattgatgtt gcacaagacc aacaaacagt aaatcggtaa cagaattttg
    63001 cgccgtgcgg gtagacatcg acgagctacg ccattcggtt tcaccggaaa cggtatcgag
    63061 cgtgaaatgc gccagccgcc gcagcggaga gtcagccaga gaagtaatgg caatcaccgt
    63121 tgccccctgc tttcgtgccg cttctgcgca caagacaatc tcttttttac tgccgctgta
    63181 agagatagcg atttgtacat cgcctttttt caacgcctgg gaaacggtgg cttgtacgtg
    63241 cgtgtcggct tcgcaggcca cgcgatagcc aattttcatc agtttgaacg acagatcgcg
    63301 ccccaccagc gccgatccgc ccaggcccgt tatctggata aacggtgctt tactgatgac
    63361 ctcaatgatt ttttgcagcc gcgcgtagtc gaacaacgca caggtttgct ccagcgccag
    63421 ctctttttcg cgattcagtt tgcgggcaat cacttccagc gaatcatcac tggtaatcga
    63481 actgtgcagg tgcagcgccg tggcattcgt tttctcccgg ctggcactgt attcgccaat
    63541 taacgccata cgcaattcag taaatccttg cgcgccgagc ttctgggcaa atttcacaat
    63601 gctcgactgg ctgataccga gctgtttcgc catctgccgt gaagagacgg atttcagctc
    63661 gctcactctg gcccgcagaa agtcagcaat tttttgctcg ttcccggtaa attcactttc
    63721 cgcattgcgt attttcgtca ggtacaacat cttccactct cctgggctgg cagcaaaaat
    63781 tccgcttaag ttcttatgac gctcttcaca ctctgcgagt gtagactcaa tgattccttt
    63841 aagtctgtca attcgggata tatgattcca tacatctgca attatttgat cgtaaatagt
    63901 aaggtcacca ccgatgcaac tagaaaagat gattactgaa ggctcgaaca ccgcctcggc
    63961 tgaaattgac cgcgtatcga cgctggaaat gtgccggatt atcaacgatg aagataaaac
    64021 cgtaccgcct gccgttgatc gcgtcctgcc ggatatcgcc gcggcgatcg atgttatcca
    64081 cgcccaggtc agcggcggcg gtcgtctgat ttatctcggt gcgggaacat ccggtcgtct
    64141 ggggattctg gatgccagcg aatgtccgcc cacctacggc gtgaaaccgg gtctggtggt
    64201 tggtttgatt gctggaggcg aatatgccat tcagcacgcg gtggaaggag ctgaagatag
    64261 ccgggaaggt ggtgttaacg atctgaaaaa tattaattta acggcacagg atgtggtggt
    64321 tggcattgct gccagcggtc gcacgccgta tgtgattgcc ggactggaat acgcacgcca
    64381 gctcggctgc cgcacagtgg gaatttcctg taatccgggg agcgccgttt caaccaccgc
    64441 tgagtttgcc attacaccga ttgtaggtgc cgaagttgtt accggttctt cgcggatgaa
    64501 agcaggtaca gcgcagaaac tggtgctcaa tatgctttcc accgggctga tgattaaatc
    64561 cggcaaagtg ttcggcaacc tgatggtcga tgtggtcgcc accaacgaaa aactgcatgt
    64621 gcgccaggtc aatatcgtta aaaacgccac cggatgtaat gccgaacaag cggaagcggc
    64681 gttaattgcc tgcgagcgca actgtaaaac ggccattgtg atggtgctga aaaatctcga
    64741 tgccgcagaa gctaaaaaac gcctggatca acacggcggc tttattcgtc aggttttaga
    64801 caaggaataa cccatggcca aagagatcag cagtgaactt ctgaacacca ttcttacccg
    64861 tgtcggcgga ccgggaaata tcgccagttg tggtaactgt atgacgcgcc tgcgtctggg
    64921 tgtacatgac agttcactgg ttgatcccaa tatcaaaacg ctggaaggcg tgaagggcgt
    64981 cattttgacc agcgaccagg tgcaggtcgt ttttgggcct ggtaaagcgc atcgtgccgc
    65041 aaaagcgatg agcgagctgc tgggcgatgc accggtacag gatgccgcag aaatcgccgc
    65101 ccagaacaaa cgtcagttaa aagccaaaca aacctccggc gtgcaacaat ttctcgccaa
    65161 attcgccacc atcttcacgc cgctgatccc cggttttatt gccgccggtc tgctgctggg
    65221 gatagcgacg ttaattgcca cggtgatgca cgttccggca gatgctcagg gcacgctacc
    65281 cgacgcgctg aattttatga aggtgttcag caaaggtttg ttcaccttcc tagtgattct
    65341 ggtgggctat aacgccgccc aggcatttgg cggcacgggc gtcaatggcg caattattgc
    65401 cgcactcttt ttgctcggtt ataaccccgc tgcaaccacc ggttactacg ccggttttca
    65461 cgatttcttt ggtctgccca tcgatccgcg cggcaatatt atcggcgtgc tgattgccgc
    65521 gtgggcctgc gcccgcattg aaggcatggt gcgccgcttt atgccggacg atctcgacat
    65581 gctgctgacc tcgttaatca ccctgctgat caccgctacg cttgcgtacc tgatcattat
    65641 gccgctgggt ggctggttat tcgaaggtat gtcatggctg tttatgcacc tgaacagtaa
    65701 tccgttcggt tgtgcggttt tagccgggct gttcctgatc gccgtggtat ttggcgtgca
    65761 tcagggcttt attcctgttt acctcgcgtt aatggacagc cagggattca acagcttatt
    65821 tcccatcctt tcaatggcag gcgcgggcca ggtgggcgcg gcgctggcac tctactggcg
    65881 ggcgcaaccg cacagtgcgc tacgcagtca ggtacgcggg gcgattattc ccggcctgct
    65941 gggcgttggt gaaccgctga tttacggtgt caccctgccc cgcatgaagc cgtttgttac
    66001 cgcctgttta ggcggcgcgg cgggcggttt gtttatcgga ttgatagcct ggtggggtct
    66061 gccgatgggc ttaaacagcg cctttgggcc gtctggtctg gtggcgctgc cgctgatgac
    66121 ttccgcacaa ggcatccttc cggcaatggc ggtttatgct ggcgggattc tggtggcatg
    66181 ggtttgcggg tttattttca ccacgctctt tggctgccgt aacgtcaatc tggactgata
    66241 ttatgaaacg gacaatgctc tatctttctc tgctggctgt tagttgtagc gtcagcgccg
    66301 caaaataccc tgttctgaca gaaggtagtt gatcaagtat atgcggcgtt aaagcagtaa
    66361 taaaaaggcc agtcagtaat gttactgcct ggcctttaat caatgaatca gaaacgctta
    66421 cagcgccatc aacttgtcca gcgacggtgc gaaataatag ccgccggtta ccggtttggt
    66481 gaaacgcaac atcgcatcac gtttgccatc ggtatcgcca aacatgctca gcagttgctg
    66541 ctcaatgtta tgcagacgcg cgcagtaggc gcagaagtac agaccgtgag tgccactggc
    66601 ggtgccgtac ggcaggctct ggcgaacaat cttcagccct ttgccatctt ctttcagatc
    66661 aacgcgggtg aggtgagagg tttccggacg ttcgtcgccg tcaatctctt cgttggcctc
    66721 tttggtgcgc ccgatcatca tctcctgatc gtgaacgctc atccggttga gctgcttcag
    66781 gttgtgctcc caacgctgca caaacacata gctgccgccg gaatccacgc cgtctttgat
    66841 aaccgccact tcgcggcgcg tctcttcacc cgccgggttt tctgtaccgt caacaaagcc
    66901 gctcaggtca cgctcttcca cccaacggaa gccgtggatc tcttctttca cttcaatgca
    66961 gtcaccaaag gcttccatcg ccgcctgagc gacagagaag tttacgtcgt gacgcagaga
    67021 aagaatgtgg atcaacacat cgaactgggt cgtcggcgca aggcctttac cgtagcccgg
    67081 aaaatctttc agctcttctg ccccaacgcc gccgctcaga gcgcgccagg tgttgttacc
    67141 aaaggcaacc accgcaccaa gatgcgcgtc cgggaatttc gcttcaaaag ttgccagttt
    67201 gtcggcaaat gttttactgg ccgcacgcag ggcgtcaact tcccctttca cgttggcttc
    67261 gatccaaatc gccgcgcggc aatgttctgg caaaatgcca ctctgaacct gagacattgt
    67321 tcctcctgaa aataagaatg ccacgcaatc cgtggcattg ttgagcgcta ttttacgtgt
    67381 tttttcgcga cggcggtttg ttcagacgca aattaacgcc gccagataat tttactgact
    67441 ttccagtttt tcagggtatc gtccgaaggc attaaccctt caggaccacg ccattccccg
    67501 ctgaactggt agctaatatg ttgactgcct tcggctttac attcgacggc acgattatca
    67561 tcaccatcag ctttttggca attgccaaat gctttgctgt aaaggtcgct aaacggtgta
    67621 ccgattttaa caccggtgtc agcaggaata tcgctatcca gcacgtcaat gcgactgatc
    67681 gtgccttgat cgccgttaat caccatcgcc acgttgtcgc ctttcatcac ttcaaaaaag
    67741 cgcaccacgt tgccgttcgc ggttttcatt ccgctgcgca ggcgataatc gccatcaagc
    67801 gcatcagcaa tggcttgttc ttgcagtggt gtggacgccg ttaattcacc cacgccctgc
    67861 tcgctcactt tggtggacga tccaaaccag ttccacgggt tagcggcaga ccagttaact
    67921 gacgacatcg tggaacagcc ggtcagcatc aacggcatag cgcataacat taaacgcagc
    67981 gatttcatgt cacttccttt ggttattcaa taacgttgct tggagtgcaa attcaccaaa
    68041 aagtgccgtt attctttttc ttcattaaaa caagcgcgta aacgtcgatt ggtcagcagc
    68101 cagattaacg ccacaatatc cgccactacc agcgccagac cgataccgct aacggattca
    68161 ccgttcagcc acagccacgg ttgccagcaa agtaaaacca cctgcgccaa cagcaacaga
    68221 aaatagagca catgccaggt gcgaggaaac gaagcccgcc gaccgctcaa caaaaacgcc
    68281 agcaccgccg gaataccggg aatcagcccc agccagaaat tatcgtgatc gggataaaac
    68341 agatttagca gcgcagtacc ctgctcacgc gacgcaccgg caatgacaaa cagcacccag
    68401 gttcgcgcct gaagcaatag cacaagccag aagagcaagg gtaaacgcag gcgtccgtgc
    68461 gcatcataat ggacaggatg gaactcagta ctcttcatct tcaatcaaac gcttacccag
    68521 actcagcacg tcggcgtgtt catatcccag gcgttcatac attccgagca ccatgtcgtt
    68581 atcttccggc acgttgatct gaattttcgg gcagccgcga gcaatcagct ttttctccag
    68641 ccggttaagc aacgcattgg caattccacg cccacgaaac tctggatgca cgccaagata
    68701 ataagcagac ccgcgatgcc cgtcataacc gcccatcacc gttccgacca cttcaccgtt
    68761 tacctcagcg accaaaaaca aactgacgtc atggttcatc ttacgctcga tgtccatttc
    68821 cggatcgttc cacggacgca gcaagtcgca acgctcccaa agggtgatga cctcttcgaa
    68881 atcttcctgg cgaaatacgc gtatctccat ggtattcgtt acctttttgc gggttaaaag
    68941 gctgattatg gcgtgaacgg tcgaattagc caatatctga cgaaaatcgg ttgaaaaagt
    69001 ggcataatgg ggagttgtca actattgaaa tgaaaagtaa aacaattctc aacagcaaac
    69061 cgtcgtaacg gattacgcga tacgatataa catctggaac tttattatta caactcaggc
    69121 cgtatgagca cttttaaacc actaaaaaca ctcacttcgc gccgccaggt gctgaaagcc
    69181 ggtttagctg ccctgacgtt gtcaggaatg tcgcaagcca ttgccaaaga cgaaccttta
    69241 aaaaccagca acggacacag caagccgaaa gccaaaaaat ctggcggcaa acgtgtcgtt
    69301 gttctcgatc caggccacgg cggaattgat accggagcga tcggacgcaa cggttcgaaa
    69361 gaaaaacatg tggtgctggc gattgctaaa aacgtccgtt ccattttgcg taatcatggg
    69421 attgatgcgc gtttaacgcg ttctggcgat acgtttatcc cactttacga tcgcgttgaa
    69481 atcgcccata aacatggcgc agatctgttt atgtcaattc atgccgatgg ctttaccaac
    69541 ccgaaagctg ccggtgcttc ggtatttgcc ctctctaacc gtggggcaag tagcgcaatg
    69601 gcgaaatacc tgtctgaacg cgaaaaccgc gccgatgaag ttgccggtaa aaaggcgact
    69661 gacaaggatc acctattgca gcaagtactg tttgatctgg tgcaaaccga tactattaaa
    69721 aacagcctga cgcttggctc gcatattctg aaaaagatta agccggtgca taaactgcac
    69781 agccgcaaca ccgaacaagc cgcgtttgtg gtgttgaaat cgccgtcggt tccttcggtg
    69841 ctggtggaaa cctcgtttat caccaacccg gaagaagagc ggctgttagg cacggcggcg
    69901 tttcgtcaga aaatcgccac agcgattgct gaaggcgtga tcagttattt ccactggttc
    69961 gacaaccaga aagcacattc gaaaaagcga taagttatga aacccgacgc acaccaggtt
    70021 aaacagtttc tgctcaacct tcaggatacg atttgtcagc agctgaccgc cgtcgatggc
    70081 gcagaatttg tcgaagatag ttggcagcgc gaagctggcg gcggcgggcg cagtcgggtg
    70141 ttgcgtaatg gtggtgtttt cgaacaggcg ggcgtcaact tttctcacgt ccacggtgag
    70201 gcgatgcctg cttccgccac cgctcatcgc ccggaacttg ccgggcgcag tttcgaggcg
    70261 atgggcgttt cactggtagt gcatccgcat aacccgtatg ttcccaccag ccacgcgaat
    70321 gtgcgttttt ttattgccga aaaaccgggt gcagagcccg tttggtggtt tggcggcggc
    70381 ttcgatttaa cccctttcta tggttttgaa gaagacgcca ttcactggca ccgcaccgcc
    70441 cgtgacctgt gccagccatt tggtgaagac gtttatcccc gttacaaaaa gtggtgcgac
    70501 gattacttct acctcaaaca tcgcaacgaa cagcgcggca ttggcgggct gttctttgat
    70561 gatctgaaca cgccagattt cgaccactgt tttgccttta tgcaggcggt aggcaaaggc
    70621 tacaccgacg cttatttacc aattgtagag cgacgtaaag cgatggccta cggcgagcgc
    70681 gagcgcaatt ttcagctcta ccgtcgcggt cgttatgtcg agttcaatct ggtctgggat
    70741 cgcggcacgc tgtttggcct gcaaactggc gggcgcaccg agtcgatcct gatgtcaatg
    70801 ccgccactgg tacgctggga gtatgattat cagccaaaag atggcagccc agaagcggcg
    70861 ttaagtgagt ttattaaggt cagggattgg gtgtaactcc ctcaccccca ttcccgcatc
    70921 cgctgatgca acgtcaacga ctgcttctcg gcaaacagct gctggtaatc cgtggcaaat
    70981 tgccccagat gccagaatcc ccactgcatg gcggcgtctt ttaccgttgt gctttgcgac
    71041 cacgggctta tcagttcgcg gcgtacggcg ttcaggcgaa tgcgtttcag ccacgcgttt
    71101 gggccaatgc ctaaaatagc gtgaaacgcg ttttgtagcg tgcggcgact gacatgcagt
    71161 tgattacaca aatccagcac cgtcaccggt tcggacatgt tttccagcac atattcacgg
    71221 gcgcgggaaa gcaaccgacg gtaactctga tgactgatgc tttccgccgt caccattggt
    71281 tgcgcttctt ccagcatcgc ccccatcgcc attagcaaat tatcccccag cacttttcgc
    71341 actgcaggct gatggagatt ttccgggttc tcgcaaaacg tcgccagcgc ctgttggaca
    71401 aagccccaca gcgcggcttt atgctgctct ttcacttcca gcgccgactg gctacgcagc
    71461 atatgtaata cccgatccgg attgtgcaaa aagttagcct gacaggtgat gacatcttca
    71521 gacagcacta cgcccaggat cgtataatcg tccggcgtac ttaattcaaa ctcggttcca
    71581 ccggggcgcg tcgctatttc tgcgcttccc agacattgcg aaccgataaa tccctgctca
    71641 ccgcgcgtcg ccggaatgcc aaaccagaac gagttcggcc agaccaggca cgactgacgc
    71701 agcgccagac cggtgtattc acgaaaaacc tgaatatcat cgagtagaat ttccgtaaat
    71761 tcaccatgaa acttgcccgg atgcagctga tcgtaaatct gctgctaggc ggtaatcgtc
    71821 aaagcatgtt catagacatc cgttgtctgt cgttgatgaa cattatccac ttcgaccttc
    71881 ggcgtgagct tcaggttttc gggtaagggt tcatgataaa gatggtgcaa attggctgta
    71941 cgggtctttt tcatgatgtt aatgccgggt gttgtaggac acccggcacc tccgacaggt
    72001 taatggggct tgagacgata acgactactg cgtttacgta acgtcccggc agaaaagagc
    72061 tgttcgagcg catttctggc tttttccagc ggccagccaa agtgggcggc cacttctcct
    72121 gccgtcattc cctgacatac tgatgttaac agcgccaaca actcgtcagc agattccgcg
    72181 acgataactg gcgcgtcggg ttcctttacg ggttgcttcg gctggcggtt aaagccagta
    72241 accagccact gggtgtcatc gtctggccga ccgatctcct tacggctgat gacacgcccg
    72301 gtacgcatcg cggcagcaca acctgcgtcc agcgccgcac gacacgccgc cagatcgcct
    72361 tccaccacca gcgttaagcg accagggtca agcacttcgt gactgagcag acgaacgtta
    72421 gctgctttga gcatggcatc cgctgcatct atcgcggcga ccatgccgtc cacttccagc
    72481 aatcccagtg cattgatcat cggcaacctc cgttacgcac gctggattgg attacgcgcg
    72541 atttccagca ctgcatcggt aaaggcgtta caggctgctt tacacgcggc ctggctaccg
    72601 gttaaaaatg ctgccgagta gttggtttca gacggtggcg ggacgtaggt caccagctgt
    72661 acgtcggcag atttcaacgc ggcatcaatg ccgtaggtcg cttccagcgg cggtgccacc
    72721 agatacgcca tcggatcgcc aagcgtgatc ccggcggttg atgaaagata agaaccggta
    72781 cgcgaaacca catgcgccag gaaggcggtg ttttctgcgt cattggccca ctggaaagct
    72841 gcgccatttt caatatgtgc aaccatcgca tccagaccgg cacgcacttc cgccgggttt
    72901 ggcccaccaa gcataatcag cacttcaccg gcagtcggtg acgggccgtg tgccgctccc
    72961 gcatacagcg agcgaccata taccacttcg accatcgctt gtttggtcgc ttcgtcagcc
    73021 gcaatgtagg ttacgtcgtc agaatcggca gaaatcagcc cgaggctacg aatatgcggc
    73081 ggcaatttta gttcacgcgc aaattcagcg ttaacggagg caatcacccg catggcggtt
    73141 accgacggtc gaatcaaatc taaagctggc atgatgcctc cttatcgggt catgttgatg
    73201 ccggatgctt tctgctccag catacgtttc gccaaatcca caatgacggc acagcagcac
    73261 gaaagagaag caaaagtcag ctacctggta cactaggcag cgggtcagca aaggggggca
    73321 aatgcccctt ttttcatgtc agataatcag atcattacct caataaacta ataatcacat
    73381 cgcggttaca accagtttga tagtgatagc tcaaacccag acgtggcgct ggctgcaagc
    73441 aaaataagcc gcaaaaatgc gaccccatgt ttactagccg caaaaatgcg gtcctatttg
    73501 ccagaacctt ttattattca attggttagt gcaagaaaaa gcgtgattac tgttcaagaa
    73561 gtatccagaa aggtcatgat tttgacgctg aaaaaaaata ggtgggcatt tttgcccact
    73621 cactagccgc aaatttgccc actcactagc cgcatttttg cggcccaaag taataaatac
    73681 agagaaataa taaatacaga tcagcgccaa gatttttcgt cttttcggcg gaatcatgag
    73741 actagtgctt acgtcctgat aacctccaga gcagacctta acttctgtgc agcaggacta
    73801 cgctcattgc cgctcggcta ggtcaaatcc tcacgataaa gctgttcgga ttgtgaccat
    73861 aatgttaaag gactctttaa atcattcctt gacgaattaa ctagctggct taagcggaat
    73921 agcatgcagg ataaaaacaa aaccagctta gcagtgggga gagcttcgct tcgctcgtct
    73981 cctggggcca atgctcaatg aaacgacgta aggcacggag cgctcaccat aggtagacac
    74041 ataaacatct gagagcgcac cttctatcgc gctacgcgct caataaaaac caacgacacc
    74101 gccacactgc aagaccaatg gtttttatcg cgtatacaga ccagacagga aacagcagca
    74161 aagcagcgta tagccaggtg cagacagcac actcacacga cgcacagcag gcttaagtga
    74221 tcactcagga aactttatcc ccgccgccga tcaccgcgaa ctatcatgac cgcgccaaat
    74281 tcgaaaggaa tggcccatag aacgcgatga acacacataa gcgctacact tacttaacgt
    74341 cgccacagag cgattgagag cgcttaaaat cgatgcgatt tcattaaatt tttctcactc
    74401 gcctgaatgg tggcgatcag gactgatcgc aacattgccc cgcctcgata ttgagcgcat
    74461 aatagataca tacattaagc ggaaaagaga taagtttatt gtgaaaatca gggggttaca
    74521 tatcatgcgc atatcagaac aacaacggca gatcctcgac tatctggcaa aaaacgaatc
    74581 aatcaccgca cgacaagccg cagatctcgt ttatggtggc aatgttaccc gcgtccaggt
    74641 tgacgccgcc cgccgctcac tcatgacgat ggttagtaat gggctaatca ggaaacaagg
    74701 ccgcgctttc gtagctgaaa gacatatcaa tcacgcttat agccaggacg ttgaagaatt
    74761 gcgccgccag atcctttggg tattcagcaa acagggtcag gagtatttcc tttctgaaca
    74821 cccaaatttc gccgccaaag agttaacagt aagcggattg tcgtgcgcac tggattatct
    74881 tgtcggtgaa ggttgcgtta cttacaagca gctatggcac gccctgaata cgcttgtaga
    74941 agatggttta ctcattgcca cgcgtgagaa catcgaatac tggataatgc cgcgttcagg
    75001 tcgccgcaat catgcacggt atatttcagg caacagatac agaacgccgg actttaatcc
    75061 gacacaggaa gatcgggacc gtgccgaaat gatgtgcagg gaacaggaga aaagaattat
    75121 ggataagtta ttttcgatgc agtgaacaca agcgggcgca atgcccgttt ttattgcctt
    75181 attccacgcc tgacgaatca atttgatcac acctgagaaa atgcatagcc agaagttaag
    75241 gccgttatat tgcgattggg agcgttttaa acggtatcaa tttaattaat tctgatgtgg
    75301 tgatttgcag gcaaaaaaaa cccgccggag aagcgggaaa tagagacata tcgttgtagt
    75361 tattaacaga tcactttgag gtcgagggcg gctaaccctc gctaatatta tcccgctatt
    75421 accttgcggg tgcaaataat cttttattgc tttgatgatt taataaatag cactttttgc
    75481 taacaaaaac tgctgtcaat atatggccaa aaatgtcaac agaaaaaata aggtttataa
    75541 tattaagtat cacatgggta atgggttcag ggaatcggaa tccaccgcct cacggaaatt
    75601 caaaatttgc ttcttctttc ctccgtgggc ggcttctttt tcgtatcgag gtatttatga
    75661 aagcaataac attagaacag ttcgcagcaa tggcgaatgt atcgaccaga acgctgaggc
    75721 gctggctaaa aaaaccatca accggagtcc ctttgccatt taccccacgc ggtactcgcc
    75781 aattattctg ggagcacgaa tgcaaagaat ggttgtacag aacagaacga taataatcac
    75841 gcatcacatt ttgaggtaaa catcatgacc aatatcgaaa gattagaacg ctgtgccgat
    75901 attatgcggc gcagatggat ttatgaccct gaagcgggtt tattgtttag ccgtgaaaca
    75961 aaagacgtta tcaaaggcag catgaccaat aaagggcatt tggttgtaac ggtacatgaa
    76021 ggtaaattca aagtgacact cacctatcag aaagcctgct atgtatacac atacggcgct
    76081 tatgacgaga cattatatga agtgcaccac gttaatttga acaaacagga taaccgcatc
    76141 aagaatattc gcctgatgcc gaaagaaaag caccgccgga tacataaaaa cgtccgcaaa
    76201 cttattcggc tgggccacac acaatggctt caaccgcaat taattgaaca atcactataa
    76261 acacaggagt aaataccatg cattcattag acagttattt tcagcgcacg acagccccta
    76321 aatcagccgc ccaggaacgc cgtgaggaat tccatgagaa agtgatgcgt tctgctgatt
    76381 atattgcgga caagttcgtg gaaacggttc gtccacttgt tgatgaagta gctgataaac
    76441 ttcagtctga aatgccggaa gatatggaag gcactgcaaa gcggcgactc atttgcgaat
    76501 taagtcgtcg ttttggcgtg tcaataagcg cttttaaata atcgaaaaaa ggggggaaag
    76561 ataatggacg catttgaggt actggcaaat aaaggcgctg aactggtagc acagcgtgat
    76621 aaaaccgcca acgaagggga acgcgcactg ctaaataaac agattaaagc gatccgtatg
    76681 gcccagttta aattaatcag taatgaagtt atcgaaacaa ttaaaccgca gatcccacaa
    76741 atcgttgctg acgctattaa agccgccgga ctggagaaac gcattgccgg gctaaaaacg
    76801 aacgcagaga aaatggagat gcacaaacag agcggtgctc cgaatcccga tgagtacttc
    76861 tcaccggatg ttgagtttat ggcgcaggtt gaggaacgtc tgggtagctg tctcaccgag
    76921 gaacagcgtc gctattttga tggagtggat agttcggcag gtattgacct gaactcgtat
    76981 ttcggtcgtg aaattgagca ttttgatgcc gccccgtcta tgccggagcc agaaccagaa
    77041 atggccaccg atcctgaagc tgagcaacgg aaacgcgtat tcaatgcgat agggattcgg
    77101 tactgatcgg ataaaagaaa gccgggaatt accccggctg acttaacaat gtgaccaatg
    77161 aacaaatcgg acaagacaaa caggataagc agtctcgaaa caatgttcgg gaactatatt
    77221 agcacaagtc cggagaaaat catcatgttg gcactacgca tcatgcaggg catcgctaag
    77281 accctcgcag aacatgtctt agacctgaaa cattcgccac tgagcaaaca ggccatgaag
    77341 cgccaaacgc ttcgtctgtg ggctgaatat tcgcttggaa ctatcaataa aatcatcgac
    77401 atgaaatcgg ggccgagcaa ccagagcgcg gaggaaatgg agttcatcag gcgtttaatt
    77461 ctcattcgca gagacattca tagccagtta cacagcgtgg gaatcgatat taatgatggg
    77521 acgggcgatt aattttcgga caagtactat gcgcgcgcga ggagctgaac atgaagcaag
    77581 atattgcaga caggctggag atactcgaag gccagcgggc agaagcgaaa caactacgta
    77641 agcaggccag aagagcgcac aggaacaacg aagcagagtt actaaccaaa tacatcagtt
    77701 tcaccaatta ctgcatctac gagtgttaca aggaagatgc agaggactgg ctggattcat
    77761 tgccagaaca gtactgagca gaataacacc acgtcatgtt gttctgaggt gagcatatca
    77821 cctctgttca ggtggccaaa tttagtgtgc cacttcagca tcaccgttga aatcacaata
    77881 acgatttttt tgtaaatatg ctctcacata tgtaggaata gaccaggttg gcattgaaac
    77941 acatctacca tgaccataat aaagtaattt tttgatatgt ttatggtttt atattaatta
    78001 aaatatatct aaaattgata gttttaaaat tgcaaaatcc agattgtgtt tgtaatgttt
    78061 aatctgattg tattgagtgg cgtttattat gaaaaataaa tcaggtaatt cttgttatag
    78121 agaagcagtc gatattcata gctcacttga gctttcattt atattgcata aatatcattt
    78181 gcgtgtagat gagaagaaat ctcgaaatgt attcagcgcc atacgtgatg agctcatcct
    78241 tgatggggat tcacgacaaa acttagctac tttctgtcaa acatggcttg atgatgaaat
    78301 ccgtgagttg cttaatttag ctattgataa aaacatgatt tataaagatg aatatccaca
    78361 aatggcggaa cctgaaggtc gctgtgtgcg tatgctttcc gattcgtgga gtttccccga
    78421 ttcgcgacac accttaggtt gctcaaccat tggttcatct gaagctgcta tgctcggccg
    78481 cttggcgtta aaatggcagt ggtgtaaaaa acgcgaagta caaggtaaat caaccgaacc
    78541 tgatttgcga tcctgtgcaa atatgctggc ataaattctg ccgttactgt gatgtgaaat
    78601 tgtgtgaaat tccacttgat gatcatcacc tgataatgaa tgctaaagag gttttgaagc
    78661 atgtcgatga aaacactatt ggtgtcgtta cgactttagg tattacttat ttttgtaaag
    78721 cagattgcgt tagtgttttt aagataagtg ttaaagttaa tattagtttt tatatttaca
    78781 tcgtaattat atgtttctat tttaaaaatg ttcgagagta taaaaatgtc taatgctaat
    78841 atcgcaccca aaagtaagaa aatgacagtg gggacactcg ctatcatgaa tataactgct
    78901 gtagtaagtc tacgatttat accttctgaa gcagaatacg gcttaggtgc cattttttac
    78961 tatacctttg ctgcaattat gtttttagta cctgttgcct ttgttgcggc tgagctagct
    79021 acaacatatc cacaaaaagg gggggcattt cgatggatag gagaagcatt tggggctcgc
    79081 tggggttttg taggaatgct tatgacctgg ctacaagtca tcccctactt tccaacagta
    79141 ctgacatttg gtgcagtctc ggttgcattt attgatgttg atatgggggt ggcagaatca
    79201 attgccagta ataaatggta tattttattt ttcgtactat ttgtttactg gggagctatt
    79261 tgtgttgcat tacgtggcgt tggtattttc tcgcaggtat ctaagtggtg cggtattata
    79321 gggacaatta ttccagctat tatccttgtt attcttggtt tctcctatct gtggttcagc
    79381 ggtaaaccag cggtgatccc attaggttgg ggtgatcttt tccctaatat agctaacttc
    79441 cagaatattg ttcttgccgc cagcatattt cttgcttatg cagggatgga aatgaatgcg
    79501 gtccatgtta acgatttaga taatccaact aaaaaatacc caattgcaat taccatcgct
    79561 tctataggta ctattgcaat attcctgctc agtactttag gggtcgcctt tattattcct
    79621 acagataaaa ttagtctaac tcaaagtttg ttggttgcct acgatatgct gttcgaatgg
    79681 gctggagtgc catggcttgg ctctgtgatg gcatttatgc tagcaattgg cgtactgggt
    79741 ggggtggtta cttggattgc agggcctaat acaggtgtat tagcgatcgc gaaagctggt
    79801 tatttaccta agtttttcca aaaaaccaat cgtcatggaa tggggcacca tctaatgttt
    79861 gtacaaggta taattgtctc agttctttct gttacttttg tgataatgcc ttcagttcaa
    79921 gcagcttttc agattttatc acagttaaca gttatgctct atttagttat gtatatgctc
    79981 atgttcgcta gtgctatcta tctacgacat tctcaacctg atacaaaacg cccatatcga
    80041 gcccctgctt tatttttctg ggcaacactt ggattcttag ggtcttttct ggcttttgta
    80101 ttaagtttta ttccgcccga gcaaatccca actggtagtc caaccagcta tgttctttat
    80161 ttgttagtat tagttgccct atttttatta attccaatat taatttacgc cttccgaaaa
    80221 tctgactgga aagatccaaa ctctgatttc gaaccatttt cttgggaaaa aagaactagt
    80281 aattaaattt tccataagct acaaaagatg aggttaaata tgtttagtgc tgaatctcca
    80341 attggtgtaa tagactcagg tgtaggtgga ttgacatcgg taaaggaaat aattaatttg
    80401 cttcctggtg aagatattat ctattgtgga gataatatga atgcacctta tggcaaccgt
    80461 tctgccgatg atatcatcag tttgactaaa aaaatgctga cttttttaca gtcaaggaat
    80521 gtcaaactag ttgctgtcgc atgtaatact atatcctcaa ctttggaatc cgaagaatac
    80581 agcggatatg ctaagagctt tccgtttcct atcttatcca tcattgaacc tgcagtagaa
    80641 gatgtgattc gccagcaata taaaaatgtt ggtattatcg ctactgagtt tactattaaa
    80701 acggggtgtc acaaagagct aataaaaaaa ttgaattcaa caataaatgt atttggagaa
    80761 ccaagcaaaa atcttgcaat gttaattgag gaaggtaatc ttaatgctcc tgcaatcttg
    80821 aatgacatca aaaaacatgt taaccattta atttccctcc atccagtaaa tgaaattatt
    80881 ctcggctgta ctcactatcc aattgttcag aatttttttg aagcagttgc accggatata
    80941 aaatttatta atcctgcaca tgatcaggct atctctatta aaaatcactt gggacagcta
    81001 aatttattaa actcctcaaa tataggaact ttgcaaataa atactagtgg aagtatggag
    81061 atctacaaaa cggtactagg ggaattgagc attacgaaac ctcatacatt cagtatcagg
    81121 cagttttaaa tttatccggt gcaccgtaaa atcccgtcct tctgaactgc accccaaaag
    81181 ttggacacca actgagtaag gtgcatttta tggacaaacc cagttacata ctgggtagtg
    81241 catccaatta gtagaacatg tgtttttcga taaacgctcc gatcactttt tcgtggatct
    81301 caactgagcg tgagaagcag attgttttac gacccaaccg cttaatgcgg gtgcgtagcg
    81361 tcagattatt acgctcaatg cgttgggtga atattttgcc ggtcagatgc ttattcttcg
    81421 gcacctcccg gccatagctg ccccagtcat cgctggtcag catgccgatg ttgaagggtg
    81481 taagcagtgc cagtagctcc cggcacgttt gatcggttcg gggaccaaaa gtgtaggcca
    81541 gtacaccgcc tgttttggtg ttgtacgcgt acccgagcca atgttgccgg gctttactgc
    81601 caacgtagct ccattgctca tcaagctcgc agataaacgc cacatcagca tgggcaacag
    81661 gcgaagaagt tattcgcttt ggcgcgagtt ttttaaagtc cggatgacgg tgttactacc
    81721 aattttcagt gtcctggcgg tatcgcgaac cccggcacca ttaaaggcca tttcagttat
    81781 cagctctttc atacccggct tacgtgcttg ataagtataa gtgaactgaa acacacggtg
    81841 gcagtcacgg cagcgaagtc tgtcacggcc tttagggttc tgaccatggc ggtaaacctg
    81901 agctgactga caacggggac aatgaatatt aacgctggtc atgagataac ctcaaaagcc
    81961 cgtattatac atcagattca actaattaga ggcatcaccc atccagcatt ataaaacata
    82021 ctaacaataa ctaaattata ataatcacaa cttacaatca aataataaat gcataagatt
    82081 aacaatgatt ttttcgggtt atatttatct aaaaaagcag aatattatgt aacttattat
    82141 tttctaagcg aaaatttatt gtgcggtcag actaacaaac ttgcgtacca cccgaagaac
    82201 atgcgaagat ttaagtattc tttctttttt ggtttttatt tttcattttc tcttttcttc
    82261 tctttccggt tctgttctgg ttttggtttt ttccttttta tttttattct ttcttcaggg
    82321 ttatgctaaa acaccaaaac atttttcacc acaaaagcat ccaatcatct tgccagacca
    82381 atcaccttaa ccggatacac tgccggatat aatcccacta acacccagac accaagcacc
    82441 atacctgata actaccgtat tcaggaagga aatgcgagtg atttcaccgt caccggcaca
    82501 accgatccgc caaaaagagg cgtaccaatg tcgatatagt cccccgcgcg gacaatcact
    82561 tcgtcaatga ctgccaacgg gagttgttgt agctgtgggc gcaacaacat gcccagagct
    82621 ttaccaaagt cctgcccggc caccaccagc aggggatgcg gattcggaaa acgcgcgacg
    82681 aaatcgacca gcgcgttgat gaccgtcaga accgcggcgt aacgcacagg cagcgaggcg
    82741 ggaagcgcca gcacgtacac gtcagttttg ggatcaagat ccagctgaag cagcgcctgt
    82801 tgccaggcac tcaccagatc cgtttcatca atcgggatcg ccaccggcaa attgcgcagc
    82861 ggcagttgta cgccctccag ccagattgtg ctgccagaga gcgaaagggt atgtgcaccc
    82921 gcgccaatca ccgtggagcg tacggtttgc gccggaaact gcacattcat ctcacgcagg
    82981 cgcggatggt catgcagcgc cgttgccaga agtgggccaa tatcggcaaa acagaacggg
    83041 ttggcgggct ggtggcgata acattcgccc acgcctccag aaagcgtaat gatttcgggc
    83101 gtaacacctg cgggcagcaa accggtttgc atcaatgcct gcgcgagcgg cgaaagcgtt
    83161 ccgtcaatca cttcgacaat cagcgccgcc atccgccggg taacctgcac cagctgcgcg
    83221 ccggtcagcg aacgggcgtc agtgcctgca ccgaagcact catccacaat catctgcccc
    83281 ggtttatgag cgtaaaccac gcgcccctgg ctgtcggttt ccagcaggcg accaccgacg
    83341 ttgagacagg cagtgccgct gatttttccg gcatcgaaca gggcgtagtt cgcggtgcca
    83401 ccgccgatat cgatattcag tacccgacac agccgttgtt cagaaagggt ttgcgccccg
    83461 gctccgtgac cggcgatcac ggattcgagg tgcggcccgg cgctggcgac gacaaaatcg
    83521 cccagcgact gtttttcctt catcactttt tccatcagct gtgcgaccag gctttcggta
    83581 aactggcctt ccggcagctg ggcaatgatg gtttcgcgga tgcgctggct ttcggttttg
    83641 tcggctggca cgctggcggg cacggacgcc gttaccggga tcgattcatc acattcggtg
    83701 atggtaaaac ccagtagatc cgccacttca cgggcttccg gggtaataat gctggcgcgc
    83761 agaaccacgg acattgcctg ttcgccgcgc gcgtgtgctt cacgaatatc attcgctgtg
    83821 atgagttttt tcacctgctt cctcctgttt ggtgagagat gccagataat ccaccagttg
    83881 ctgcacgctt tgcgggtcgt ggctgtttag ctcgaaaatc ggctcttcga aacccgtctc
    83941 aagcaataac tttcgcgttg cggcgacgtc agcatctggc atgtccgttt tactgatgac
    84001 ggcgatttgc cgtttgctga cgccaatatc cagcaacccg gcaggcaacc gactttccgg
    84061 gtcatttgcg ccatggacat agatcagcat gtctacatcc tgtagcgtgg taattaaggc
    84121 gtgataccag cggggatggc taaaatattc acccggcgta tcaatatcgc ccttatcatt
    84181 aaattccacg gccagtgttt ttctggcgag ggtataattc ccctgcaacg cattaaataa
    84241 cgttgttttt cctgccccga cgctacccac aaacgcaatt cgtttcatga atgc
//
LOCUS       DAEEUE010000003        78636 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.003, whole genome shotgun sequence.
ACCESSION   DAEEUE010000003 DAEEUE010000000
VERSION     DAEEUE010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 78636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78636
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.003"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            499..1114
                     /locus_tag="KLK84_000192"
                     /pseudo
     CDS             499..1114
                     /locus_tag="KLK84_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188400.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1217..1540
                     /locus_tag="KLK84_000193"
     CDS             1217..1540
                     /locus_tag="KLK84_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HBE2007681.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1607..3064)
                     /gene="nuoN"
                     /locus_tag="KLK84_000194"
     CDS             complement(1607..3064)
                     /gene="nuoN"
                     /locus_tag="KLK84_000194"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HBE2007682.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3071..4600)
                     /gene="nuoM"
                     /locus_tag="KLK84_000195"
     CDS             complement(3071..4600)
                     /gene="nuoM"
                     /locus_tag="KLK84_000195"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HBE2007683.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4764..6605)
                     /gene="nuoL"
                     /locus_tag="KLK84_000196"
     CDS             complement(4764..6605)
                     /gene="nuoL"
                     /locus_tag="KLK84_000196"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HBE2007684.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVFSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6602..6904)
                     /gene="nuoK"
                     /locus_tag="KLK84_000197"
     CDS             complement(6602..6904)
                     /gene="nuoK"
                     /locus_tag="KLK84_000197"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HBE2007685.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6901..7455)
                     /gene="nuoJ"
                     /locus_tag="KLK84_000198"
     CDS             complement(6901..7455)
                     /gene="nuoJ"
                     /locus_tag="KLK84_000198"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HBE2007686.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7467..8009)
                     /gene="nuoI"
                     /locus_tag="KLK84_000199"
     CDS             complement(7467..8009)
                     /gene="nuoI"
                     /locus_tag="KLK84_000199"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HBE2007687.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8024..9001)
                     /gene="nuoH"
                     /locus_tag="KLK84_000200"
     CDS             complement(8024..9001)
                     /gene="nuoH"
                     /locus_tag="KLK84_000200"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HBE2007688.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8998..11724)
                     /gene="nuoG"
                     /locus_tag="KLK84_000201"
     CDS             complement(8998..11724)
                     /gene="nuoG"
                     /locus_tag="KLK84_000201"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HBE2007689.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11777..13114)
                     /gene="nuoF"
                     /locus_tag="KLK84_000202"
     CDS             complement(11777..13114)
                     /gene="nuoF"
                     /locus_tag="KLK84_000202"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBE2007690.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13111..13611)
                     /gene="nuoE"
                     /locus_tag="KLK84_000203"
     CDS             complement(13111..13611)
                     /gene="nuoE"
                     /locus_tag="KLK84_000203"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HBE2007691.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13614..15416)
                     /gene="nuoC"
                     /locus_tag="KLK84_000204"
     CDS             complement(13614..15416)
                     /gene="nuoC"
                     /locus_tag="KLK84_000204"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HBE2007692.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15510..16172)
                     /gene="nuoB"
                     /locus_tag="KLK84_000205"
     CDS             complement(15510..16172)
                     /gene="nuoB"
                     /locus_tag="KLK84_000205"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HBE2007693.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16188..16631)
                     /gene="nuoA"
                     /locus_tag="KLK84_000206"
     CDS             complement(16188..16631)
                     /gene="nuoA"
                     /locus_tag="KLK84_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HBE2007694.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17262..18200)
                     /gene="lrhA"
                     /locus_tag="KLK84_000207"
     CDS             complement(17262..18200)
                     /gene="lrhA"
                     /locus_tag="KLK84_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HBE2007695.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19120..20337
                     /gene="alaA"
                     /locus_tag="KLK84_000208"
     CDS             19120..20337
                     /gene="alaA"
                     /locus_tag="KLK84_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HBE2007696.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20421..21020
                     /gene="yfbR"
                     /locus_tag="KLK84_000209"
     CDS             20421..21020
                     /gene="yfbR"
                     /locus_tag="KLK84_000209"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HBE2007697.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21079..22911)
                     /locus_tag="KLK84_000210"
     CDS             complement(21079..22911)
                     /locus_tag="KLK84_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBE2007698.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22998..23648)
                     /gene="hxpA"
                     /locus_tag="KLK84_000211"
     CDS             complement(22998..23648)
                     /gene="hxpA"
                     /locus_tag="KLK84_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HBE2007699.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23659..24153)
                     /locus_tag="KLK84_000212"
     CDS             complement(23659..24153)
                     /locus_tag="KLK84_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HBE2007700.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24236..24691)
                     /locus_tag="KLK84_000213"
     CDS             complement(24236..24691)
                     /locus_tag="KLK84_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HBE2007701.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25029..26231
                     /gene="ackA"
                     /locus_tag="KLK84_000214"
     CDS             25029..26231
                     /gene="ackA"
                     /locus_tag="KLK84_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBE2007702.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26306..28450
                     /gene="pta"
                     /locus_tag="KLK84_000215"
     CDS             26306..28450
                     /gene="pta"
                     /locus_tag="KLK84_000215"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBE2007703.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28640..30160
                     /gene="yfcC"
                     /locus_tag="KLK84_000216"
     CDS             28640..30160
                     /gene="yfcC"
                     /locus_tag="KLK84_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBE2007704.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30193..30735)
                     /gene="yfcD"
                     /locus_tag="KLK84_000217"
     CDS             complement(30193..30735)
                     /gene="yfcD"
                     /locus_tag="KLK84_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HBE2007705.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30793..31344)
                     /gene="yfcE"
                     /locus_tag="KLK84_000218"
     CDS             complement(30793..31344)
                     /gene="yfcE"
                     /locus_tag="KLK84_000218"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBE2007706.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31400..32044)
                     /gene="yfcF"
                     /locus_tag="KLK84_000219"
     CDS             complement(31400..32044)
                     /gene="yfcF"
                     /locus_tag="KLK84_000219"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HBE2007707.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32180..32827
                     /gene="yfcG"
                     /locus_tag="KLK84_000220"
     CDS             32180..32827
                     /gene="yfcG"
                     /locus_tag="KLK84_000220"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HBE2007708.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32884..33246
                     /gene="folX"
                     /locus_tag="KLK84_000221"
     CDS             32884..33246
                     /gene="folX"
                     /locus_tag="KLK84_000221"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HBE2007709.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33267..34160
                     /locus_tag="KLK84_000222"
     CDS             33267..34160
                     /locus_tag="KLK84_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HBE2007710.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34208..35098)
                     /gene="rpnB"
                     /locus_tag="KLK84_000223"
     CDS             complement(34208..35098)
                     /gene="rpnB"
                     /locus_tag="KLK84_000223"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HBE2007711.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35295..36068)
                     /gene="hisP"
                     /locus_tag="KLK84_000224"
     CDS             complement(35295..36068)
                     /gene="hisP"
                     /locus_tag="KLK84_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HBE2007712.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36076..36792)
                     /locus_tag="KLK84_000225"
     CDS             complement(36076..36792)
                     /locus_tag="KLK84_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE2007713.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36789..37475)
                     /gene="hisQ"
                     /locus_tag="KLK84_000226"
     CDS             complement(36789..37475)
                     /gene="hisQ"
                     /locus_tag="KLK84_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HBE2007714.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37565..38347)
                     /gene="hisJ"
                     /locus_tag="KLK84_000227"
     CDS             complement(37565..38347)
                     /gene="hisJ"
                     /locus_tag="KLK84_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HBE2007715.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38568..39350)
                     /gene="argT"
                     /locus_tag="KLK84_000228"
     CDS             complement(38568..39350)
                     /gene="argT"
                     /locus_tag="KLK84_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HBE2007716.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39616..40185)
                     /gene="ubiX"
                     /locus_tag="KLK84_000229"
     CDS             complement(39616..40185)
                     /gene="ubiX"
                     /locus_tag="KLK84_000229"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HBE2007717.1"
                     /translation="MKRLIVGISGASGAIYGVRLLRVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40280..41797)
                     /gene="purF"
                     /locus_tag="KLK84_000230"
     CDS             complement(40280..41797)
                     /gene="purF"
                     /locus_tag="KLK84_000230"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBE2007718.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41834..42322)
                     /gene="cvpA"
                     /locus_tag="KLK84_000231"
     CDS             complement(41834..42322)
                     /gene="cvpA"
                     /locus_tag="KLK84_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HBE2007719.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42581..43243)
                     /gene="dedD"
                     /locus_tag="KLK84_000232"
     CDS             complement(42581..43243)
                     /gene="dedD"
                     /locus_tag="KLK84_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HBE2007720.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43233..44501)
                     /gene="folC"
                     /locus_tag="KLK84_000233"
     CDS             complement(43233..44501)
                     /gene="folC"
                     /locus_tag="KLK84_000233"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBE2007721.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44571..45485)
                     /gene="accD"
                     /locus_tag="KLK84_000234"
     CDS             complement(44571..45485)
                     /gene="accD"
                     /locus_tag="KLK84_000234"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HBE2007722.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45641..46300)
                     /locus_tag="KLK84_000235"
     CDS             complement(45641..46300)
                     /locus_tag="KLK84_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBE2007723.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46383..47195)
                     /gene="truA"
                     /locus_tag="KLK84_000236"
     CDS             complement(46383..47195)
                     /gene="truA"
                     /locus_tag="KLK84_000236"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBE2007724.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47195..48208)
                     /locus_tag="KLK84_000237"
     CDS             complement(47195..48208)
                     /locus_tag="KLK84_000237"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBE2007725.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48274..49410)
                     /gene="pdxB"
                     /locus_tag="KLK84_000238"
     CDS             complement(48274..49410)
                     /gene="pdxB"
                     /locus_tag="KLK84_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HBE2007726.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49509..50504
                     /gene="flk"
                     /locus_tag="KLK84_000239"
     CDS             49509..50504
                     /gene="flk"
                     /locus_tag="KLK84_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HBE2007727.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50501..51679)
                     /locus_tag="KLK84_000240"
     CDS             complement(50501..51679)
                     /locus_tag="KLK84_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE2007728.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(51934..53175)
                     /gene="fabB"
                     /locus_tag="KLK84_000241"
     CDS             complement(51934..53175)
                     /gene="fabB"
                     /locus_tag="KLK84_000241"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HBE2007729.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53334..55340
                     /gene="mnmC"
                     /locus_tag="KLK84_000242"
     CDS             53334..55340
                     /gene="mnmC"
                     /locus_tag="KLK84_000242"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HBE2007730.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55461..55739)
                     /locus_tag="KLK84_000243"
     CDS             complement(55461..55739)
                     /locus_tag="KLK84_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HBE2007731.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55773..56321)
                     /gene="epmC"
                     /locus_tag="KLK84_000244"
     CDS             complement(55773..56321)
                     /gene="epmC"
                     /locus_tag="KLK84_000244"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HBE2007732.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56321..57130)
                     /locus_tag="KLK84_000245"
     CDS             complement(56321..57130)
                     /locus_tag="KLK84_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBE2007733.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57130..57954)
                     /gene="mepA"
                     /locus_tag="KLK84_000246"
     CDS             complement(57130..57954)
                     /gene="mepA"
                     /locus_tag="KLK84_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HBE2007734.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(57958..59043)
                     /gene="aroC"
                     /locus_tag="KLK84_000247"
     CDS             complement(57958..59043)
                     /gene="aroC"
                     /locus_tag="KLK84_000247"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBE2007735.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59078..60010)
                     /gene="prmB"
                     /locus_tag="KLK84_000248"
     CDS             complement(59078..60010)
                     /gene="prmB"
                     /locus_tag="KLK84_000248"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBE2007736.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            60176..60727
                     /gene="smrB"
                     /locus_tag="KLK84_000249"
     CDS             60176..60727
                     /gene="smrB"
                     /locus_tag="KLK84_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HBE2007737.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60831..61721)
                     /locus_tag="KLK84_000250"
     CDS             complement(60831..61721)
                     /locus_tag="KLK84_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HBE2007738.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61708..62232)
                     /locus_tag="KLK84_000251"
     CDS             complement(61708..62232)
                     /locus_tag="KLK84_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE2007739.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62229..62699)
                     /locus_tag="KLK84_000252"
     CDS             complement(62229..62699)
                     /locus_tag="KLK84_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE2007740.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62696..63244)
                     /locus_tag="KLK84_000253"
     CDS             complement(62696..63244)
                     /locus_tag="KLK84_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE2007741.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63249..63971)
                     /locus_tag="KLK84_000254"
                     /pseudo
     CDS             complement(63249..63971)
                     /locus_tag="KLK84_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(63991..64713)
                     /locus_tag="KLK84_000255"
     CDS             complement(63991..64713)
                     /locus_tag="KLK84_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBE2007742.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(64773..66005)
                     /locus_tag="KLK84_000256"
     CDS             complement(64773..66005)
                     /locus_tag="KLK84_000256"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBE2007743.1"
                     /translation="MRADWQTNYQHTRSNDDDEFSGDETQKKWEWSRYYARRALPSLK
                     AKLALGEDYLRSDIFDGFNYVGGSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQM
                     GRVIYETQVPAGPFRIQDLGDSVSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVR
                     YKIMMGRPQEWGHHVEGEFFSGAEASWGIANGWSLYGGALGDENYQSAALGVGRDLST
                     FGAVAFDVTHSHTKLDKDTAYGKGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENF
                     MTMSEYLDASDSGMVRTGNDKEMYTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYN
                     IMLSHYFNMGSIRNVSISMTGYRYEYDNQADKGMYISLSMPWGDNSTVSYNGTMAVGR
                     TAVRSVISAVSMTRLTIS"
     gene            complement(66054..66632)
                     /locus_tag="KLK84_000257"
                     /pseudo
     CDS             complement(66054..66632)
                     /locus_tag="KLK84_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499769.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(66714..67277)
                     /locus_tag="KLK84_000258"
     CDS             complement(66714..67277)
                     /locus_tag="KLK84_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBE2007744.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
     gene            complement(67961..68446)
                     /gene="sixA"
                     /locus_tag="KLK84_000259"
     CDS             complement(67961..68446)
                     /gene="sixA"
                     /locus_tag="KLK84_000259"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HBE2007745.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(68649..70793)
                     /gene="fadJ"
                     /locus_tag="KLK84_000260"
                     /pseudo
     CDS             complement(68649..70793)
                     /gene="fadJ"
                     /locus_tag="KLK84_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
     gene            complement(70793..72103)
                     /gene="fadI"
                     /locus_tag="KLK84_000261"
     CDS             complement(70793..72103)
                     /gene="fadI"
                     /locus_tag="KLK84_000261"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HBE2007746.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(72283..72567)
                     /locus_tag="KLK84_000262"
     CDS             complement(72283..72567)
                     /locus_tag="KLK84_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HBE2007747.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            72939..74279
                     /gene="fadL"
                     /locus_tag="KLK84_000263"
     CDS             72939..74279
                     /gene="fadL"
                     /locus_tag="KLK84_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HBE2007748.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            75030..75704
                     /locus_tag="KLK84_000264"
     CDS             75030..75704
                     /locus_tag="KLK84_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2007749.1"
                     /translation="MIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGKSEPTDLF
                     TWYGKDKKGDSLAIVIKNKNGNDYLFLGYYDQDDYHIQRGIRINGDSLTQYCSENARS
                     ASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDDAKF
                     RRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTKYQNEINQLSALG
                     VNFDDI"
     gene            complement(75886..76641)
                     /gene="mlaA"
                     /locus_tag="KLK84_000265"
     CDS             complement(75886..76641)
                     /gene="mlaA"
                     /locus_tag="KLK84_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HBE2007750.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            76935..77867
                     /locus_tag="KLK84_000266"
     CDS             76935..77867
                     /locus_tag="KLK84_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBE2007751.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHAELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            77941..78015
                     /locus_tag="KLK84_000267"
     tRNA            77941..78015
                     /locus_tag="KLK84_000267"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:77974..77976,aa:Arg,seq:cct)
     gene            78177..>78636
                     /gene="intS"
                     /locus_tag="KLK84_000268"
     CDS             78177..>78636
                     /gene="intS"
                     /locus_tag="KLK84_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HBE2007752.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
BASE COUNT        19909 a        21014 c        19300 g        18413 t
ORIGIN      
        1 aatcaatgct tcttatacac aattagaaat aaaacactca caacccaaaa atagagcatc
       61 cggcaaaaga gtgagttaac atcattgtta atcaattcga ccagtcagta aatatgctga
      121 ctggtcgaac ataatttgct aaaaagtcat cactgactga tgtcagtccc accatctgcc
      181 agttcaatct acgggtattt ccagcatatc gcggcacgcg attgctggtc tgttgatgta
      241 gtggattgta gttgctgact tttcttaccc tcagatttct gccccacttg tcaattcaat
      301 ctgccaataa ataacataat tatattttta ttcctcacag aaattccttt ttaaaaatag
      361 cgaacccact gtaaaaaaca ctttttgtat acctctacca agcgtaataa aataaacgct
      421 cataattcat tataaataat aatgaaataa cgccttttat tgataattta catatgaaaa
      481 aaattttcct tttgttatat tatttctgtt ttcactccct ctgattattt ttaccaaccc
      541 tgggtcactg cattatcgcc aacgccccga cacgtaagtc ctgagcaatt agaaaaaaca
      601 gttcgttatc ttacacaaac tgtgcatcca cgtaatgccg gcaatattga taatctgaat
      661 aggtccgcag agtatataaa agagttcttt gtcagtagcg gtgccagagt taccgcgcag
      721 ggcgtccccg attggctatt acgactctgc gcctggaagc cagaattacc cttacccggc
      781 aatgtcctgg ctttatcccg atcggggaga ctttattgct gtggtcggca gaatgcagga
      841 tatcaacgcc gttcgtcagg taaaagcggc attgttgtca tcccgggatt tatctgttta
      901 ttctataaat gccccagggt ttattcccgg tattgatttt tctgatcacc tcaattattg
      961 gcaacacgat attcccgccg taatgattac tgacaccgct ttttatcgta ataaacaata
     1021 ccacttgccc ggtgataccg cagacagatt gaattatcag aaaatggctc aggtggtaga
     1081 tggtgttata accttgttat acaacagtaa ataataaaaa tttctcgagg cgggtccaga
     1141 aacagcgaca tgtacatttt attttccctc gtctgtttta agatgcgaaa cataaaattc
     1201 aatggaggaa aaataaatgg gaatgattgg ctattttgca gaaattgact cagaaaagat
     1261 taaccaactg cttgagtcta ctgaaaaacc tttgatggat aatatacatg acacgctttc
     1321 aggattacgc cgactggaat cactggatcg aagtgagctg cgtaaacagt tttccattaa
     1381 gcgactcaat gaaatggaga tttatccggg agtgaccttt tcagaagagt tagaggggca
     1441 actctttgcc tcgatcatgc ttgatatgga aaagctgatt agcgcgtatc gacgtatgct
     1501 gcgacaaggg aatcatgcgc tgacggttat tgttgggtga tggtgtcaga gcgccaaaaa
     1561 aaaccgccga acatgtcggc ggctttctga cttacaaact aacagtttac atcagcggca
     1621 ttgctaaccg cacaatacta atcagcggtt gtggccatac acccagcacc agtaccaaca
     1681 gtgcagaaat cagcaccacg ataccgcccg cgctgtactg ccagtttgac ggtgcatcgc
     1741 gacccggttg ttccggtgcg tgcaggtaca ggctcaccgc cacgcgcagg tagtagtaga
     1801 ggccgattgc cgaaccgaca accacggcac ccaccagcca ccacaagtgt gcctggacac
     1861 cgaccgccag cacgtagaac ttaccgataa agcccagcgt catcgggata ccggccagag
     1921 acagcatcat caccgtcatc actgccgcga ggatcggacg atgccagaac agaccgcggt
     1981 aagagaacag ggaatcagca tccgggccac gatacgggct ggacatcagg ctgaccacgc
     2041 cgaacgcgcc gaggctgctg aacagataac cggccaggta aacccctacc gcttccatcg
     2101 acatctcgcc ggtttgcagc gcaatcagcg ccaccagcag atagccgagg tgagagatag
     2161 atgagtaacc gagcagacgt ttgatgttgg tctggctcaa cgccatcagg ttaccgaaga
     2221 tgatggaggc aaaggcgata atcgccagta ctacgcgaat cgcttcgctg tcacccaccg
     2281 gtgcgtagag gaacagacgc atcaccacac cgaagatagc gattttgctc gccgtcgcca
     2341 ggaaagtgga aaccggcgca ggcgcgccct ggtatacgtc tggcgtccac aggtggaacg
     2401 gcaccagaga gagtttgaag ccgaggccaa caatcatcat gccgaaacct gccagcaaca
     2461 gcggctcgtt gagcataccg tcgccgaggt ttttacccag cgcgacaaac gacaggtcgc
     2521 cagactgcgc atacaccagc gccataccga acagcaggaa agaagacgct gcggcagaaa
     2581 ggatggtgta tttgatactg gcttccagcg aacgtttctg gcggaaagcg taaccgacca
     2641 ggccaaacag cggcaaagag atcagttcaa taccgaggaa cagagacgcc agatggtttg
     2701 cattcgccag caggatcccg cccagcgcgg caattaacac caacaggtag aactcatcct
     2761 tgttgtcgtt atagccttca agccacgggt aggcgaaagt acaggtggcg aggctcgcca
     2821 acaataccag cccggtgtaa agcatggcga aaccatcaac gcgcatcagc ggcgtaacgt
     2881 ccatagcgcc cgcctggcca acaaaccaga gcgaaaccag cgccgcgtta agcccaataa
     2941 ccgagagcgt agcgttgagg aaatgattgc gtcgccacgc aatggagagc atcacaacca
     3001 ccaccgtcaa gccgacgatc agcaacggta gcagtgcgat caggttttgt ggagttattg
     3061 tcatggcgat ttacggcctt gtagtagtaa cggaattaac aaaccactgc tggatattgc
     3121 caatcgcgga gtgcgaggta tccagaatcg gctgcggata gaagcccagc agtaccagca
     3181 gcaccaccag caacaggatc ataaacagct cacgcagcga catccctggc agttcctggc
     3241 tggcaatctg gcttttcgct ttaccgaagt aagcgcgatg caacatcgcc agcgaataaa
     3301 cagatgcaaa gaccagccca aaggtagaga taacggtaat caccgggaca acctggaagc
     3361 tgccgaacag aatcataaat tcgccgacga agttaccggt gccaggcatc ccaagcgttg
     3421 ccaccgcaaa gaacagcgac agtgctggca gccatttcat cttgctccac agaccgccca
     3481 tcatgcgcat gtcgcgggta tggatacgtt cataaagctg accgcagaga ataaacagac
     3541 ccgccgccga caagccgtgc gcaatcatct ggattaccgc gccctggtag gccaactggc
     3601 tgccggtgta gattgcaatc agcacgaagc ccatgtggga aaccgaggtg taggcgatca
     3661 gacgtttgat atcggtctga gcgaaggtca tccacgcacc gtagaagatg ccgataacac
     3721 ccagccacat agcgattggc gcgaactctg ccgacgcgtt cgggaacagc ggcagggaga
     3781 aacgcaacaa accgtaagcg gcggttttca gcaagatccc cgcgaggtcc acggaaccgg
     3841 cggtcggagc ctgggagtgc gcatccggca gccagccatg cagcggaacc accggcattt
     3901 tgactgcgaa ggcgatgaag aagcccagca tcaacagata ttccacacca ctggacattg
     3961 gcgtattcag cagctcttca tagttgaagg tccagacgcc ggtcgcattg tagtgaacaa
     4021 aaaccagcgc caggatggcg atcaacatca ccagaccact cgcctgggtg taaatgaaga
     4081 acttggttgc cgccgtgata cgcgttttac cgtcagaggc tttatgcccc cacagtgcga
     4141 tcaggaagta catcggcacc agcatcattt cccagaagaa gaagaacagg aacatgtcga
     4201 tggcaaggaa cacgccgata acgccgccca ggatccacat caggttgagg tggaagaagc
     4261 cctgatattt ttcgatctct ttccacgaac atagtaccgc cagcacaccg agcagaccgg
     4321 tcagcacgac catcagcagc gacagaccgt caatggcgag atgaatagag ataccaaaac
     4381 gcgggatcca cggcatgtcg aattcagact gccactgcgg aattccggcg gattgcgtca
     4441 gtgaataacc gccctgcaac cacagttgca gcgacagcgc cagcgtcaat cccatggtga
     4501 tcagcgcgat ccagcgtggc accttgacgc caaagcgttc ggtctgccag cacaggaagc
     4561 cgccaataaa gggaattaat attagccagg gtagtaacat ggcgatcttt attccttgta
     4621 aaagtcccgt caggaccgga ttttcaacga attctcacga cagaaaattc tcttcggact
     4681 gggggtagtg tcggatgcgc cgcgaaggca gcttatccga cctacgttct tatatgccca
     4741 ggggcaatcc cacaatcctt aactcaacgc agtaccatca acagtgccag caccacgacc
     4801 gcaccgatgc tcatggatgc cacataccag cgcagataac cgttctcgct taacagcaga
     4861 cctttacctg caaagcggga aaaaaccgcc gggatgttca tcattgagtt cagcggatcg
     4921 cgtttcagca accaggcaat acccaggaac ggcttgacga acactttgtc gtacagccag
     4981 tcaaagcccc aagcgttgta ccaccaggta ctcagcaggc ggcccggcgc actgttggcg
     5041 atggaggtca ccagagaacg tttacccagc cacagccagg ctgccagcag aatgccgacc
     5101 accgcgacca cgccagaggt aatttccagg gtcaacatgc tgccgtgcgc cagttccgtc
     5161 gtttgcggaa gcacgccctg cagcggcggt acaatcagtg cgccaacgaa ggtggaaagg
     5221 atcagcagca caatcagcgg caggctgtga gttacccctt tcacggcgtg agcgtgaatt
     5281 tgttcttttc cgtggaagac gatgaaaatc atacggaagg tgtagagcga ggtcataaac
     5341 gcaccgacca gacctgccac catcagattg atatgaccat tcgccatcgc acccgcgagg
     5401 atctcatcct tactgaagaa gcccgcagtg accagcggca gtgccgacag tgctgcgccg
     5461 cccaccagga agcagagata aaccagcgga atagatttac gcagaccgcc catcttgaag
     5521 atgttctgtt cgtgatggca ggccagaatg acggaaccgg atgccaggaa cagcagcgct
     5581 ttaaagaacg cgtgagtcat caagtggaaa atcgccgcat cccatgcctg cacgccaagc
     5641 gcgaggaaca tgtagccaat ctggctcatg gtagagtaag cgagaacacg tttgatgtcg
     5701 gtctgtacca gcgcggcaaa accggccagc agcagcgtaa ccgccccgac aatacccacc
     5761 agatgcagaa cttccggcgt catcaggaac aggccgtggg tacgggcgat caggtagaca
     5821 cccgcggtta ccatcgttgc ggcgtggatc agcgcggaga caggcgtcgg gcccgccatc
     5881 gcgtcagcaa gccatgtctg caacggcaac tgcgcagatt taccgaccgc accgcccagc
     5941 agcatcagcg tcgcccacat cagcatgtta ttgccgtcag caaagtgcgc tggtgccagt
     6001 tccaccattt cgcggaagtt cagggtgccc agttcgttgt aaagaatgaa cagtgcgaaa
     6061 gcgaggaaaa cgtcacccac acgggtcacg acgaacgctt tcattgctgc tgcgccattc
     6121 ttcggatcgg tgtaatagaa accgatcagc agataggagc acaggcccac gccttcccag
     6181 ccgaggtaca tcagcagcag gttatcggcg agaaccagaa ctaccatgct ggcgatgaac
     6241 aggttggtgt aggcgaagaa gcgagagtag ccctcttcac cgcgcatgta ccaggaggcg
     6301 tacatgtgaa taaggaaacc cacaccagtg accaccgaga gcatggtcag cgacaggccg
     6361 tccagcacca ggttaaaacc gatgttaaag tcgcctaccg acatccacgt ccacagcggc
     6421 tggctgtatg cctgctcgcc gttagcgaag aaatcaacgc cgataaaggc ggttaccagc
     6481 gccgccaggc ccacagagcc aacgccgacg atcgccgaaa cgttttcaga ccagcgccca
     6541 cgggagaacg ccagcaggac gaagccaatc aatggcaaaa taatggttaa ggcaagcatg
     6601 ttcatccgcg catctcactt actgaatcga tgttcaggtt ctggcgacga cggtgaagtt
     6661 gcagcagcag cgcaaggccg atactcgctt ctgccgccgc gaggctgatg gcgagaatgt
     6721 acatcacctg accgtcggtc tgaccccagt agcttccggc gaccacaaag gccagcgcgg
     6781 aggcgttaat catgatttcc agaccaatca gcataaacag cagattgcga cggataacca
     6841 gaccggttaa gccaagaacg aataagattg ccgcgaggat cagtccatgt tgtaagggga
     6901 tcatgcgtgc tcctccgttt ttcttttcgc gctgtcgtct ttacgattgc tcagcacttc
     6961 acccgcacgc tcttcacgac cgacgtggaa ggccacaacc agacccgcga gcagcagcat
     7021 agaagccagt tccaccgcca gtacgtaagg cccgaacagc gtaataccca ctgctttagc
     7081 actgattggc gtaccgtcga taccctgatc gttaacaccg aggatggcgt aaacaatcac
     7141 caccagcatg atggccgaca aaattgccgg gccaatccac acctgcggtt tcagccactg
     7201 gcgttcctgt tcgatttctg aaccgcccag gttgagcatc atcaccacga acacgaacag
     7261 caccataatg gcacccgcgt agacgataat ttccagcgca ccggcgaagt aagcgcctag
     7321 tgagaagaac accccggaaa tcgccagcag cgaaataatc aggtacagca gtgcgtgtac
     7381 cggattggta tgggtgatca ctcgcaaggt cgcaagtatg gctatcaggc cacagatata
     7441 aaaagcgaac tccattgccc ctctccttac ggtaacaggc tcttgacgtc gataggcttg
     7501 gcttcgttct ctgcttcgcc cttatctttg ccgtcgattg ccatacctgc catccggtag
     7561 aagttatatt ccgggtattt gcccggaccg gagatcagca gatcctcttt ctcgtaaacc
     7621 agatcctggc gcttgtattc ccccatttcg aaatccgggg ttaactgaat cgccgtggtc
     7681 ggacaggctt cttcgcacag accacagaaa atgcagcgtg agaagttaat gcggaaaaat
     7741 tccggatacc agcgaccgtc tttggtttct gctttttgca gcgagataca gccgaccggg
     7801 caggctaccg cacagaggtt acaggctacg caacgctctt cgccgtccgg gtcgcgggtc
     7861 agaacgatac gaccacgata acggggcggc agatagaccg gctcttccgg gtacattcgc
     7921 gtttcgcgtt tggcgaacgc gtgcaggccg atcatccaga tactacgaac ctgggtgccg
     7981 aaacctacta acaattcttt taaggtcatg gtcttattgc cccttattgc gcctgccaga
     8041 gaatgacagc cgccgttacc agcaagttga tcagcgtcag cggcaggcag attttccagc
     8101 cgaaggacat tacccggtca taacgcggac gcggtaacga cgcacgaatc aaaatgaaca
     8161 tcatcataaa gaacgcggtt ttcagcgcga accagatgaa tggcggtaac aacgggcctt
     8221 gccagccacc gaagaacagc gtcaccatca atgcagagat ggtcacaatc ccgatgtact
     8281 cacccacgaa gaacagaccg aacttcatac cggaatattc aatgtggtaa ccatccgcca
     8341 gttcctgctc ggcttccggc tggtcaaacg ggtgacggtg acacaccgcc acgcccgcga
     8401 tggcaaaggt aataaaacca aagaattgcg ggatgacgtt ccacacatgc gcctggctgt
     8461 tgacgatgtc ggtcatgttg aatgaaccgg cctgcgccac cacgcccatc agggaaagcc
     8521 cgaggaacac ttcgtagctc agggtctgcg cagaagcacg catcgcaccc agcagcgagt
     8581 atttgttgtt acttgaccag cccgcaaaca gcaccgcgta aaccgccaga cctgccatca
     8641 tcaggaagaa caaaatcccg atgttcaggt cggcaaccac ccaacccgga ctgactggca
     8701 caattgcaaa ggccagcagc agcgaggtaa aggcaatcat cggtgccagg gtaaagatga
     8761 cgcgatccga gaatttcggg atccagtctt ctttaaagaa cattttgatc atgtccgcaa
     8821 ccagctggag cgaaccgccc cagccaacac ggttaggtcc ataacggttc tggaacagac
     8881 ccagcaggcg acgttcgcca aagctcatga atgccccaca ggtgacaacc accagcagga
     8941 tcaccaccgc tttgaggatg gtcagcagga tctcaatcag ttccggtgat atccaactca
     9001 ttgttgtgcc tccttgagat cctcaagatg cgcgccagcc agcaccggag caatgccgga
     9061 catacccatc ggcaagccca cctgccctgc cgtcagtcct tcggcgattt caaccggcag
     9121 cgtgaccgtg ttgccatcgt aactaaagga gacgcgtgta cctgcgttca cacccaactt
     9181 cgcggcatcc gctgggttga gtttgatgta cggctgcggc atacggctct ggaagaccgg
     9241 agcacgctgt gacaattcat cgctgccaaa caggtggtag tacggcgcga tacgccattt
     9301 cccgtcctgc ggctggaagc gtgccggtac gctggtgaag taatccagac cgttttcgct
     9361 ggtttcaaac agacgcacgc ccggatcgcc aaagcgcagt ttgccgccca cttcgtcctg
     9421 gaatttgttc cacgcctgcg gggagttcca gcccggcgcc caggcaaacg gcacttgcga
     9481 acggtgcgcg gtcggctggt tgttaccttc catcgagaag gtgaacatgg tgtcaatatc
     9541 ctgcggctga cgcggctcat gaacgctgat attggcgcgc atggcggtac gaccgctgta
     9601 acggtgcggt tcacgggcca gtttctgccc acgaatacgg aatgtcgcat ccggcgcagc
     9661 atctttgata cccgccagtt ccgggatttt cgccacaaca gcgtcaatta catggtcgag
     9721 ctgcgtccag tccacttcac ggctcagcaa ggtgctgtgc agcgagtgca gccagcgcca
     9781 gctttccagc atgacagttt tgctgtcgta ataagccgga tcgtaaacct ggaagaaacg
     9841 ttgggcgcga ccttcattat taattaccgt accgtcgctt tcagcaaagc tggcagagga
     9901 aagcaccaga tgggcgtttt ccatgatcgc tgtgcgttga tgatcaacca ccatcaccag
     9961 cggcgcttta gccagcgcag cattcacgcg ggtagcagaa gcgtaacgat gcagatcgtt
    10021 ttccaacacc accaccgcgt cggcgcgtcc ggtttccagt tcggttaacg cttcttcaag
    10081 cgaaccgcca cccataatgc ccagccccat gctgttgacg gaacgggcaa tcatggtgat
    10141 accaacgtca gcgccgcgac ctttcagggc tttcgcgacg ttagccgcag cctgaatcac
    10201 ctctatgcta ccggcgttcg tcccggagat aatcaacggt ttcttcgcac ctgccagtgc
    10261 ctgcacgatg acgtcgattt tgttttgcag ctcaggttcg ataccgtcaa ccgctggtgc
    10321 ggagttatcc agcgcatggg cgatggcaaa acctaaacgc gcctgatctt caaccggtgc
    10381 gcggtaagtc cacgccgcga tatcatccag acgagtgtca tcaacgttgg taacaaacag
    10441 cggatgcttc gcacgttgac cgatgttgag gattgccgca atctgccagt cagccacttt
    10501 ctgtgctgca gccatttcgc gcgctttacc tttcaccgcc tgacgcactg ccagtgcgac
    10561 gcgcgcgccg gtctgggtaa cgtcttcgcc cagcaccagt accgcatcgt aagattcgat
    10621 ttcgcgcaga gccggagtat aaatgccgcc ttcacgcagc actttcagcg ccagttgcag
    10681 acgttcctgc tcaccgtgag cgataccggt gtagaagttt tcttcgccca ccagttcacg
    10741 cagcgcaaag ttgctttcca cgctggcacg cggagaacca ataccgatca ctttcttcga
    10801 ctgacgcaga atatctgccg cgccctgcat tgcctgttcg gcgttgaggg taatgaaatc
    10861 atcgccacga cgctgtactg gctgacgcgg acgatccttc aggttgacgt aaccgtaacc
    10921 gaaacgacca cggtcgcaga ggaagtagtg gtttaccgta ccgttgtaac ggttttcgat
    10981 acgacgcagt tcgccgtaac gttcaccggg gctgatgtta cagccgatgg aacattgctg
    11041 gcagatgctc ggcgcaaact gcatatccca tttacggttg taacgctcgg agtgcgtttt
    11101 gtcggtaaat acgccggtcg ggcaaatttc gaccaggtta ccggaaaatt cgctttccag
    11161 cgtgccgtct tccgggcgac cgaagtagac gttgtcgtgc gcgccataaa cgcccagatc
    11221 tgtaccgtca gcgtaatctt tgtagtaacg cacacagcgg tagcaggcga tgcagcggtt
    11281 catttcgtga gagatgaatg gccccaaatc ctgattacgg tgggtacgtt tggtgaaacg
    11341 gtagcgacgg aagctgtgtc cggtcatcac ggtcatatcc tgaagatggc agttaccgcc
    11401 ttcttcacat accggacagt cgtgcgggtg gttggtcatt aaccactcga ccacactttc
    11461 acggaactgt ttcgcttctt cgtcgtcaat ggaaataaag gtgccatcgg aagccggtgt
    11521 catacaggac atcaccaggc gaccacgcgt gtcttccgcg ttttggtatt gcttcaccgc
    11581 acactggcgg caagcaccga cgcttcccag cgccggatgc cagcaaaagt aaggaatatc
    11641 aaggcccaga gacagacaag cttccagcag gttgtccgct ccgttgacct cgtattcttt
    11701 gccgtctaca tgaattgtag ccattagcat gcttccagtt ttctcggtca gggaccgagc
    11761 gttaatcgaa attcggttac cagcgctctt tcagcaggtt cggctgaatc ccattaatca
    11821 aatgggtatt gctgaacggc tgtttgattc ccgcctcaaa ttcttcgcgg aaatatttga
    11881 tggcgctctg taacggctcc actgcaccag gtgcgtgggc acagaaagtt ttacccgggc
    11941 ctaagaatcg acacagttgc tcaagtgttt cgatatcgcc cggctggcct tcgccacgct
    12001 ccagcgcacg cagaattttc acgctccacg gcagaccgtc gcggcacggc gtacaccagc
    12061 cgcaggactc acgggcgaaa aactcttcca ggttacgcac cagcgacacc atgttgatct
    12121 catggtcaac cgccatcgcc agcgccgtac ccagacggct gcccgcttta ccgatacttt
    12181 cgaattccat cggcagatca aggtgcgctt cggtcaggaa gtcagtcccc gcgccgcctg
    12241 gctgccaggc tttaaatttc agaccatcac gcataccacc ggcgtaatct tcgaggatct
    12301 cgcgtgcggt ggtgccgaac ggcagttccc acagtcccgg atttttcacc cgaccggaga
    12361 agcccatcag cttggtgcca gcatctttac ttttcgagat gttctgatac cactccacgc
    12421 cgttagcgag gatcgccgga acgttacaca gggtttcgac gttgttgaca caggtcggtt
    12481 tgccccatac gccggaggtt gccgggaagg ggggtttcga gcgtgggtta gcacgacgcc
    12541 cttccaggga gttgattaac gctgtttctt ccccgcagat gtagcgccct gccccggtat
    12601 ggacgaacag ttcgaagtcg aaacctgttc ccataatgtt tttgccaagc aagcccgctt
    12661 cagtggcttc ggcaatggcg cggcgcagat ttactgccgc ttcgatatat tcgccacgca
    12721 ggaagatgta gccacggtaa gctttcagcg caaacgcgga gatgagcata ccttccacca
    12781 gcaggtgcgg cagttgctcc atcaacaggc ggtctttata ggtgcccggc tccatttcat
    12841 cggcgttaca cagcaggtaa cggatgttca tggattcgtc tttcggcatc aggctccact
    12901 tcaggccagt cgaaaagccc gcgccgccgc gccctttcag accagcgtct tttacctgat
    12961 taacgatttc gtccggagac agcccggtca gcgccttacg cgccccttcg taaccgtttt
    13021 tgctgcggta ttcgtccagc cacactggct gtttgtcatc gcgcagacgc caggtcagcg
    13081 gatgcgtttc gggagtacgg ataatgtttt tcatttatac cgctccagca gttcagggat
    13141 cgcttccggg gtcagatgcg cgtgagtgtc ctcatcgatc atcatgtttg gccctttatc
    13201 acagttcccc aggcagcaag ttggcagcag cgtaaagcgg ccatcaaatg tcgtttgccc
    13261 tggtttgatg ttcagctttt tctcgagcgc cgcctgaata ccctgatacc cgttgatatg
    13321 acagaccacg ctgtcgcaat aacggatcac atggcgacca accggctggc ggaagatctg
    13381 actgtagaac gttgccacac cttcgacgtc gcttgccgga atacccagca catcggcgat
    13441 cgcgtggatc gcaccatccg gcacccagcc acgctgcttc tgaacgattt tcagcgcttc
    13501 aatggacgcc gcacgcgggt cttcgtagtg gtgcatctcg tgctcaatcg cttcacgctc
    13561 tgccgcactc agctcaaaag cctcggtttg tggttgttga ttctcgtgca taattagcgg
    13621 tccacatctg acataacaaa atcgatactg cccagataaa caatcaggtc agacaccagg
    13681 ctgccgcgga tcgccgccgg aatttgctgc aaatgcgcat aactcggcgt gcggatacga
    13741 gtgcgataac tcatggtgct accgtcgctg gtcaggtagt aactgttgat ccccttggtc
    13801 gcctcaatca tctggaaaga ttcattggca ggcatcaccg gcccccacga cacttgcagg
    13861 aagtgggtga tcagggtttc gatatgttgc agcgtgcgct ctttcggcgg cggcgtggtc
    13921 agcgggtgat ccgctttgaa cgggccttcc ggcatgttgt tgaggcactg ctcaagaatg
    13981 cgcagactct ggcgcagctc ttccactttc agcattacgc gggtgtagca gtcagaaaca
    14041 ccaccaccaa ccgggatttc aaagtcgaag ttttcatagc cagaataagg acgcgcctta
    14101 cgcacgtcga agtcgatccc ggtagcacgc aggcccgcgc cagtggtgcc ccactccagc
    14161 gcctctttcg cgccataggc ggcaacgccc tgggaacgac ctttcagaat ggtattttgc
    14221 agcgccgctt tctcgtaaga cgccagacgt ttcggcatcc agtcgaggaa ctcacgcagc
    14281 agacgatccc agccgcgcgg caggtcgtgc gctacgccgc caatacggaa ccacgccggg
    14341 tgcatacgga aacccgtgat cgcttccacc agatcgtaaa ttttctgacg atcggtaaag
    14401 gcgaagaaca cgggcgtcat tgcgccgacg tcctgaataa aggtcgagat gtacagcagg
    14461 tggctgttga tacggaacag ttcggagagc ataacgcgaa tgacgttaac gcgatccggc
    14521 acggtgatcc cggccagttt ctctaccgcc agcacgtaag gcatttcgtt aacgcagccg
    14581 ccgaggtatt caatacggtc ggtatacgga atgtagctgt gccaggactg gcgctcgccc
    14641 attttctccg caccacggtg gtggtagccg atgtctggta cgcagtcgac aatctcttcg
    14701 ccgtcgagtt gtaaaacgat acggaaagcc ccgtgcgccg acgggtggtt cggaccgagg
    14761 ttgaggaaca tgaagtcctc gttttcggtg ccgcgcttca tcccccactc ttccggtttg
    14821 aaggtcaggg cttccatctc cagatcctgt ttggctttgg tcagctcaaa cggcgagaat
    14881 tcggtagcgc gcgccgggta atctttacgc agcgggtgac ctttccaggt ttgcggcatc
    14941 atgatgcggc gcaggttcgg gtgaccgtcg aaagtaatgc caaacagatc ccaggtttca
    15001 cgctcatacc agttagcgtt cgggaacagt ttggtgaagg tcggtacgtg caggtcgttt
    15061 tctgccagcg ccaccttcag catgatgtcg cggttacgat cgatagaaat cagatggtag
    15121 aaaacggaaa aatccgcggc aggtaaccct tcgcggtgtg tgcgcagacg ttcgtccatg
    15181 ccgtgtaagt caaacagcat gacgtaaggt ttcggcagtt tctttaagaa atcgccaact
    15241 tccagtaatt gttcacgctt gatccacaca acgggaaccc cggtgcgagt cgcctgaaca
    15301 gtaaaggcat ccggcccaaa acggttgcgc agttcgccaa ttaccggatc atcaagatga
    15361 tcgcgggtct gccaggcggg ttcttgcgcg gttaagtcgg tcatattgtt caccattgca
    15421 aatggtccgt ggtgactgtc ggcgcagtaa gcttcgcgaa atagatttag gaataagcga
    15481 agaaaaatcc ctttgccgac aggcgcaaat taaatctcgt caggtgtacg caggttggtt
    15541 acggcaatgc gttcaccgcg cttgcgttcg cgctctgatt gcatattggc gcgataaacg
    15601 ccctgatcgc caaccaccca ggagagcgga cgacgttctt tgccgataga ttcctgcaac
    15661 agcatcagtg cctgcatgta cgcttcagga cgcggcgggc agcccgggat atacacatca
    15721 accgggatga atttatcgac gccctgcaca acggaataaa tatcgtacat accaccagag
    15781 ttggcacagg cacccattga gataacccat tttggttcca gcatctggtc atacagacgc
    15841 tgaataaccg gtgccatttt ggtaaagcag gttcctgcaa ccaccatcag gtcagcctga
    15901 cgcggcgaag cacgcaatac ttctgcgcca aaacgcgcca cgtcatgcac cgcggtaaac
    15961 gaagtcacca tctcaacgta acagcaggaa agaccgaagt tatacggcca aattgagttt
    16021 ttacgacccc agttaaccat gtcattgagc ttgcccataa acacgttttt gttaacttct
    16081 tgctccagag ggtcggttac gatctcctgc ttttgcaggg ggtaacggtc gttctcaccg
    16141 ttgggatcta tgcgggtgag cgtataatcc atcttaatgc ctcgcggtta gcgttgacga
    16201 ttagcgatac tgttcgtttc cgggttcata cgctcgcggc gtgaacgcgc gggcgtccag
    16261 tccagcgcgc caatacgcac cagataaacc agacctgcca gtaacacaaa aataaaaatt
    16321 gcagcttcca caaagcctac ccagccgctt tcgcggatag aggttgacca tgcgaacaga
    16381 tacagcgctt caacgtcgaa gataacgaag aacatggcca ccagataaaa cttggcggac
    16441 aggcgtaagc gggcggagcc gaccgagtcg ataccggatt caaacggcac gtttttcgac
    16501 ctcgcgcgtg cgcgaccgcc taaaaaccaa ccgcctacca gcatcaggca acacaggcca
    16561 atggcaacga taagaaagat agcgaatgcc cagtgatgag cgatgacttc agtggatgtt
    16621 gacatactca ttgcttactc atcaaaagta gcgccagatt cactgttctt cacggcagat
    16681 ggacgccaca tcgattcatg gggaggaata aaaaaaacct tacaataact gtagaaattc
    16741 ttttatacag ctaattgatg tggtctttta ctcctttcta taaccttttg tcaactttaa
    16801 caaaagcttc ttcacattag tttacataat atcaacacca ttagcattta atgccctttc
    16861 accccagatc cttgacgact ccaggataat tagatgttgt tgaatcgtgt ccgttgtgaa
    16921 gcaatggaaa aaatacgggt ctattttgac aggaatttgt gtcgattcct ccccccaaaa
    16981 gagagtattt tcttgatctg tgacacgctt ttgttattcc ataacaaaaa cgcagcaaca
    17041 aatttacgta ttttttaaca tcattgtagc aggtgatttt tttcaggcga ttatttgtgc
    17101 attcgggacg tgaatctctg gtgattgaaa aataaacagt tttgtacgtt ctgcactatg
    17161 cgaggaagga tttttactaa aaaaaagccg ctggggttta aaacaccccc agcggctcgt
    17221 tttttacact attgtctcag gaattatcta tcgtccgtcg attactcgat atccctttca
    17281 atcaacaatg aatcatcccc ttccggagca gacattgggc tgtattgcca cggattatgg
    17341 tagctctcca tcgcctgata gatcacctgg gccagctcat tattactgga cgggtcatag
    17401 cacagcaaat attcggtatc aggtagcggc ggtaagccat ctactccact cagcacacgc
    17461 aggtccgggc tcatcatctc aaccggcctt gccgtcacgc caagaccggc tttcactgcc
    17521 gcacgaacgg ccggaagcgt cgaggcgaca taagccagtc gccatggaat atctacttta
    17581 ttaagcgtcg ccagcaccat atcgcgaaac ggacttggat catccagcaa tacaagaggg
    17641 atcggctcgc ctttttgcaa tacgtattcc gctgcgcagt accagtgtgt tggcgaggta
    17701 cgcaggttca atgccttaaa cgctgaagga cgatgggtgg ttaccatcaa atccacttcc
    17761 tgggattcca gcatttctgc catataggca ttacgtttaa cgcggacatc cagcgctaat
    17821 ttcggataaa ccgaactcac gcgatttaac aggaaaggta agatcgtatc ggcagattca
    17881 tctgaagcac cgatagttaa cacgccctga agattactga acattaatga tgagcaggcc
    17941 tcatcattaa aacgcaggat tttcctggcg taaccaagaa gttgaatgcc atgttcagtt
    18001 aacagtttgt tgcgaccgtg acgagcgaac agttctttcc caacgagttg ttccagacgc
    18061 tgcatttgct gacttacggc ggactgagta cgacacacag cggcagctgc ggcagcaaaa
    18121 gtgttcagat cggcaacagc aacaaatgtt ctcagcagat cgaggtcgag gttaattatc
    18181 ggacgatttg cacttatcat atattatcac ttactggcgg ctcatactga gctggttaat
    18241 gctgtgcaca cacaaacaag caattccatt tgtaatgtgc ctccctggca gtttcatccg
    18301 gaacccggac gaaagtaaaa atgcatatga gttgcactaa aaaagcgact cacattgttc
    18361 cgttataatg cctgaagtag atcacagaat atatcttcag ggatcgcata tctattaagt
    18421 tactcactct tttctattta tgacatgcgc gtgtttgtat aaatgtaaat gtgagtcctt
    18481 gttccactct cgtgcagcat cgctggtcat acgcgaacac gtacaaacag caatggtgaa
    18541 gcggcatcaa gagcagggat ccgtcattta tgcgagcatt ttaccccaaa aaccttttat
    18601 ttataagggt cattgcgaat tatctgatgc aaagttatgt tatgttaggc aaagtaatct
    18661 tctattatta ataagcacat caaaaccttt ttgaatatta aataataatt aattagcatc
    18721 atcctcattc attaattccg cttaacaata gtttcacacc ttacccccat tgcgacacct
    18781 cggaaatcat cacgctgtta aactctccgt aatgcggaaa tatcatcatc gcgtttcatt
    18841 agtgaattct taaatgaggc attttcacac aattatctta cagataaaaa aaccagactt
    18901 gcaattaaga attagaacaa ttcactatat aacattgcat gtaaagcata tacacctcat
    18961 tattttgtca ttattaagtt attaacagca caatcgagcc ttcccctctg gcaaaatctt
    19021 attctgcaga ccttcaaaac accgtcctgg gggagtacat tgttctaagc tgacttccac
    19081 ggcagggagt ggcgataaca gcaaaaaagg tcaagattca tgtcccccat tgaaaaatcc
    19141 agcaaattag agaatgtctg ttatgacatc cgtggtccgg tgctgaaaga agcaaaacgc
    19201 ctggaagaag aaggtaacaa ggtactgaaa ctgaacatcg gcaacccagc cccattcggt
    19261 tttgacgcgc cagatgaaat cctcgttgac gtgatacgca acctgcctac cgctcaaggg
    19321 tattgcgatt ccaaagggct ttactccgcg cgtaaagcca tcatgcagca ctaccaggct
    19381 cgtggcatgc gtgatgttac cgtggaagat atttacatcg gcaatggtgt atcggagctt
    19441 atcgttcagg caatgcaggc attgctgaac agcggggacg aaatgttggt tcctgcacca
    19501 gattacccac tctggaccgc ggcggtttcg ctttccagcg gtaaagcggt gcattatctt
    19561 tgcgatgaat cctctgactg gttcccggac ctcgatgata ttcgcgctaa aattacgcct
    19621 cgtacgcgtg ggattgttat tatcaaccca aataacccaa ccggcgcggt atattccaaa
    19681 gagcttttaa tggagattgt ggagattgca cgtcagcata atctcattat cttcgccgat
    19741 gaaatttatg acaaaattct ctacgacgat gctgagcatc actcaattgc gccgctggca
    19801 cctgacctgc tgaccattac ctttaacgga ctgtcgaaaa cgtaccgcgt tgcaggcttc
    19861 cgtcaggggt ggatggtgtt gaacgggccg aaaaaacacg ccaaaggcta catcgaaggt
    19921 ctggaaatgc tggcttcaat gcgcctgtgt gctaacgttc ctgcgcaaca cgccattcag
    19981 accgcgctgg gtggttatca gagcatcagt gaatttatta cccctggcgg tcgtctttat
    20041 gagcagcgta accgcgcgtg ggaactgatc aacgatattc cgggcgtttc ctgcgtgaaa
    20101 cctcgtggtg cgctgtatat gttcccgaaa atcgacgcca aacgctttaa cattcatgac
    20161 gatcagaaaa tggtgctgga tttcctgttg caggaaaaag ttctgttggt gcaagggacg
    20221 gcattcaact ggccgtggcc ggatcacttc cgcattgtca cgctaccgcg tgtcgatgat
    20281 atcgagctgt ctttgagcaa gttcgcgcgt ttcctttctg gttatcatca gctgtaatct
    20341 taatttcact gccggagatt gcatccggca gtgttatccc gccacaatga cctgatgatg
    20401 tcatcatacg taaggtcact atgaaacaga gccatttttt tgcccatctc tcccgcctga
    20461 aactcattaa ccgctggccg ctaatgcgca acgtgcggac ggaaaatgtg tccgaacaca
    20521 gtttgcaggt agcgatggtc gcccatgcgc tggcagctat caaaaatcgg aaatttggcg
    20581 gtaatgtcaa cgccgaacgt atcgctttac tggcgatgta ccacgatgcc tcagaagtgc
    20641 tcaccggcga tctccctact ccggtgaaat acttcaatgc gcaaatcgct caggagtaca
    20701 aggctattga aaaaatcgct cagcagaaac tggtcgatat ggttccggaa gaactgcggg
    20761 atatctttgc gccgttaatt gacgagcatg catatagcga tgaagaaaaa tcgctggtga
    20821 aacaggcaga tgcactgtgt gcatatctga aatgtctgga agaactcgcg gccggaaata
    20881 atgaattctt gctggcaaaa acgcgactgg aagcgacgct tgaagcgcgt cgcagccagg
    20941 agatggacta cttcatggaa gtatttgttc ccagcttcca tctttcgctc gatgagatta
    21001 gccaggattc accgctgtaa gcagccggag tctgcgtcgc atcagtcaat aagcgccgga
    21061 tgcgacatca ggctcttgtc aaaacggaaa cagcatcggg atcatcacca cacaaaccgc
    21121 catcacgata atggtgaacg gtacccccaa cttcacaaag tcactgaagc tgtaatttcc
    21181 cggacctaaa accagggtgt taacaggtga agacaccggt gtcataaagg cggcggatgc
    21241 tgccatcgcc acgaccatcg caaatggata aggcgacact cccatcgttt tggcagcagc
    21301 cagcgcaatc ggagccatca acaccgccgt cgcggtatta gagataaaca gcccaataac
    21361 cgccgacaag acaaacaaac accccagcat catatgtggc ccataaccgc cgccaatatc
    21421 catcagccct ttcaccgcca gcgcgacacc tcccgttttc tgtaatgcca cagcaaacgg
    21481 catcatccca acgatcaaaa taatgctcgg ccagtgaatg gatttatagg cgctttcagc
    21541 atctatacag cggaatttcc ccatcagcag gcaggcgatg atagcggcaa caggattagg
    21601 aatttcattt gtcagcatta acgccaccat tagcaccaga cagaaaatgg catggggtgc
    21661 ctggctgtgc gcgggcgatg cttcactcac ctcttccggt aagttcagcg ctacgaagtc
    21721 gcggccctgt ttggccagca taccgatcag tttccagtta cccacaacca ggatgatatc
    21781 gcccagcagc agaggctcat ccgccagcga accttccagc gccacgccat tgcgcttgag
    21841 ccccaccaca ttcagtccgt agcgggtacg aaaaccaatt tcgcgcaccg atttaccaat
    21901 cagttctgac tcaggaatta atgaaatctc tgccatgccc acatcaaggg cctggtcaga
    21961 aaaatactcg ccgcgcagta ccatcggctc cagcaattgc tcactacaaa attgccggag
    22021 atcgacatca gccgcagaca tatcaataag caaaacgtca cgcgcgcgaa attcagaaac
    22081 cccattaacg ttcacgataa cgcgacgaaa acgccgccag cgttcaacac cgatgacgtt
    22141 agcgccataa cgctcacgta atttgagatc atccagccgt tgacctatca ttggcgatcc
    22201 ggggcgaata gccagacgtc gcgcacgccc ggtcagtcga tattcacgga taagatcgcg
    22261 aaaggttcga cgcgtccagc cttcgcgctg cggggtctgg gtatcccctt tcagcatgaa
    22321 acgcatcact aacatataca aaatacccag caccagcacg accaggccaa taggtgttac
    22381 gctaaagaaa ctgaagccgt gatagccttc acgcagcaat tcactgttga ctaccaggtt
    22441 cggcggcgtc gccaccagcg tcatcatgcc gctaatcagc ccggcaaaac ttaacggcat
    22501 catcagacgt gacggcgaag tttgcatacg catggcaacg cttaacacca cgggaataaa
    22561 gatagcgaca acgctggttg aactcataaa cgcgccaagc cccgcgacgg tcagcatcaa
    22621 caaaaccaac attttgattt cactgttgcc cgccactttg accagccatg ttcccattac
    22681 ggtggcaaca ccggtacgga ccaaaccatc gccaataata aacaaggcgg caatcaggac
    22741 aacgttagga tcagaaaagc cggaaaatac ttcggggacc gtcagcgttc cgcttaatgc
    22801 aaacgcgaca ataacaaaca aagcgaccgc atccatacgc actctgcccg tcgcaaacaa
    22861 gacgatggca acggccagta atgaaagaac ccaaatcaat tcaccgttca caacttatcc
    22921 ttgttaattg agggggatga cttgattctg ccataaaaaa gccccgacga gacggggcta
    22981 aatcatgatc aggtgtttca ctgaataata acatcgccat ttggctgttt ggtcacagta
    23041 atttgctcca gactgtggag gaccaaatcg acctcattca ggcgcggggt atctgccgga
    23101 gcgttaaccg caatgacatg gcaacccgcc gccaggccag aaagcacgcc agcgggagca
    23161 tcttccacca ccacacactc ctgcggcgca agccccagca gctgcgcacc taacagatac
    23221 gcatcaggtt ctggttttcc gcgcttcact cgctcagcgg ttacaaacac ctctggtgcg
    23281 ggaagccccg ctattttatg gcgcgctcgc gctaccggca tggagccaga agtcacaatg
    23341 gcccacggaa tacctgcttt attcaaatga ttgagtaagg cgattgcccc aggaagcgcg
    23401 gtaatacctt cggtttccgt ggcctcgatt tgctccagac gcgtaaactc ggcggcaata
    23461 tcagcctcgg atttgcccgc cataaaatgg cgcaaagagg tgatcgcctg tttaccgtga
    23521 atgaaagcca gcacctcttc cggcgctaac ccatgacgtc tggcccagtt gctccacgcc
    23581 cgttctaccg caggcaggga atccaccagc gttccatcaa gatcaaacag aaaacctttg
    23641 caccgcacgc gggcctcctc aggcgttgat aatttgatta atttcgttgg cgctcaaatg
    23701 gtactgacgc gggcaggcat gccacacatt aagcatgcgc tgatattttt cccacattgg
    23761 cgtctgggcg ttaaaaccat gagtcccagc gtcaaaatgg gtatagcgcc cttccacatt
    23821 aaccataaag cggacataac cgaggtaacg tgcttcagtg gcggcgtcaa agccgaggaa
    23881 ggtgacacga cgttcatcga tggattgctg gtcctgcaaa ttagaccagg aaacatacaa
    23941 cgcatgatac atctccataa tgtcgatgat agtgcggcag gtttcttctt tcagctcgcc
    24001 aaactcgcga tccaattcac gcatctgtaa tccgtaacca cgctcaataa ttgtttgcag
    24061 gcgacggtaa cgttcagcat ttgccggatc gagcatagtc atcatcttgt actgattaga
    24121 caaaataaga cgttgcgcgt tggtcatttc cattgttgac tcctgtatca ctctactacg
    24181 gtgaaaaaaa agaaggctga gtatgccttc ttttatatgc gtaatcaggg gtcaattaca
    24241 aatcatcaag gaaagtttta tccagttgtt tgaaggcgcg cttaagcgtg tcagctaatg
    24301 cctggtaatc aggcttgcct tcaacgggtg ccaacacctg tccagactcc tgcaatttac
    24361 cgcgaacttc ataaaaccag ttaaggattg cagggggtaa tggcgttaca gaacgcttgc
    24421 ccagccacca caatccctgc atgggtaaac ttaaggcgaa cagtgcagtg gcaactgccg
    24481 gcccaagcag accgcccagg gcaatctgcc agcagagagt aaatacggcg atcggcggca
    24541 taaaacggat cgcataacgc gtcatcttga taacgcgatt ttcgacaaag accggggcaa
    24601 ggcgtttttc cagtggccac gtctttgagt aatgctgtcc gcggcgaaac aagctaaaaa
    24661 aattaacaga acgattatcc ggcgttgaca tgcttcacct caacttcaca tataaagatt
    24721 caaaaatttg tgcaaattca caactcagcg ggacaacgtt caaaacattt tgtcttccat
    24781 acccactatc aggtatcctt tagcagcctg aaggcctaag tagtacatat tcattgagtc
    24841 gtcaaattca tatacattat gccattggct gaaaattacg caaaatggca tagactcaag
    24901 atatttcttc catcatgcaa aaaaaatttg cagtgcatga tgttaatcat aaatgtcggt
    24961 gtcatcatgc gctacgctct atggctccct gacgtttttt tagccacgta tcaattatag
    25021 gtacttccat gtcgagtaag ttagtactgg ttctgaactg cggtagttct tcactgaaat
    25081 ttgccatcat cgatgcagta aatggtgaag agtacctttc tggtttagcc gaatgtttcc
    25141 acctgcccga agcacgtatc aaatggaaaa tggacggcaa taaacaggaa gcggctttag
    25201 gtgcaggcgc cgctcacagc gaagcgctca actttatcgt taatactatt ctggcacaaa
    25261 aaccagaact gtctgcgcag ctgactgcta tcggtcaccg tatcgtacac ggcggcgaaa
    25321 agtataccag ctccgtagtg atcgatgagt ctgttattca gggtatcaaa gatgcagctt
    25381 cttttgcacc gctgcacaac ccggctcacc tgatcggtat cgaagaagct ctgaaatctt
    25441 tcccacagct gaaagacaaa aacgttgctg tatttgacac cgcgttccac cagactatgc
    25501 cggaagagtc ttacctctac gccctgccgt acaacctgta caaagagcac ggcatccgtc
    25561 gttacggcgc gcacggcacc agccacttct atgtaaccca ggaagcggca aaaatgctga
    25621 acaaaccggt agaagaactg aacatcatca cctgccacct gggcaacggt ggttccgttt
    25681 ctgctatccg caacggtaaa tgcgttgaca cctctatggg tctgaccccg ctggaaggtc
    25741 tggtcatggg tacccgttct ggtgatatcg atccggcgat catcttccac ctgcacgaca
    25801 ccctgggcat gagcgttgac gcaatcaaca aactgctgac caaagagtct ggcctgctgg
    25861 gtctgaccga agtgaccagc gactgccgct atgttgaaga caactacgcg acgaaagaag
    25921 acgcgaagcg cgcaatggac gtttactgcc accgcctggc gaaatacatc ggtgcctaca
    25981 ctgcgctgat ggatggtcgt ctggacgctg ttgcattcac tggtggtatc ggtgaaaatg
    26041 ccgcgatggt tcgtgaactg tctctgggca aactgggcgt gctgggcttt gaagttgatc
    26101 atgaacgcaa cctggctgca cgtttcggca aatctggttt catcaacaaa gaaggtaccc
    26161 gtcctgcggt ggttatccca accaacgaag aactggttat cgcgcaagac gcgagccgcc
    26221 tgactgcctg atttcacacc gccagctcag ctggcggtgc tgttttgtaa cccgccaaat
    26281 cggcggtaac gaaagaggat aaaccgtgtc ccgtattatt atgctgatcc ctaccggaac
    26341 cagcgtcggt ctgaccagcg tcagccttgg cgtgatccgt gcaatggaac gcaaaggcgt
    26401 tcgtctgagc gttttcaaac ctatcgctca gccgcgtacc ggtggcgatg cgcccgatca
    26461 gactacgact atcgtgcgtg cgaactcttc caccacgacg gccgctgaac cgctgaaaat
    26521 gagctacgtt gaaggtctgc tttccagcaa tcagaaagat gtgctgatgg aagagatcgt
    26581 cgcaaactac cacgctaaca ccaaagacgc tgaagtcgtt ctggttgaag gtctggtccc
    26641 gacacgtaag caccagtttg cccagtctct gaactacgaa atcgctaaaa cgctgaatgc
    26701 ggaaatcgtc ttcgttatgt ctcagggcac tgacaccccg gaacagctga aagagcgtat
    26761 cgaactgacc cgcaacagct tcggtggtgc caaaaacacc aacatcaccg gcgttatcgt
    26821 taacaaactg aacgcaccgg ttgatgaaca gggtcgtact cgcccggatc tgtccgagat
    26881 tttcgacgac tcttccaaag ctaaagtaaa caatgttgat ccggcgaagc tgcaagaatc
    26941 cagcccgctg ccggttctcg gcgctgtgcc gtggagcttt gacctgatcg cgactcgtgc
    27001 gatcgatatg gctcgccacc tgaatgcgac catcatcaac gaaggcgaca tcaatactcg
    27061 ccgcgttaaa tccgtcactt tctgcgcacg cagcattccg cacatgctgg agcacttccg
    27121 tgccggttct ctgctggtga cttccgcaga ccgtcctgac gtgctggtgg ccgcttgcct
    27181 ggcagccatg aacggcgtag aaatcggtgc cctgctgctg actggcggct acgaaatgga
    27241 cgcgcgcatt tctaaactgt gcgaacgtgc tttcgctacc ggcctgccgg tatttatggt
    27301 gaacaccaac acctggcaga cctctctgag cctgcagagc ttcaacctgg aagttccggt
    27361 tgacgatcac gaacgtatcg agaaagttca ggaatacgtt gctaactaca tcaacgctga
    27421 ctggatcgaa tctctgactg ccacttctga gcgcagccgt cgtctgtctc cgccagcgtt
    27481 ccgttatcag ctgactgaac tggcgcgcaa agcgggcaaa cgtatcgttc tgccggaagg
    27541 tgacgaaccg cgtaccgtta aagcagccgc tatctgtgct gaacgtggta tcgcaacttg
    27601 cgtactgctg ggtaatccgg cagagatcaa ccgtgttgca gcgtctcagg gtgtagaact
    27661 aggtgcaggc attgaaatcg ttgatccaga agtggttcgc gaaagctatg ttggtcgtct
    27721 ggtcgagctg cgtaagaaca aaggcatgac cgaaaccgtt gcccgcgaac agctggaaga
    27781 caacgtggtg ctcggtacgc tgatgctgga acaggatgaa gttgatggtc tggtttccgg
    27841 tgctgttcac actaccgcaa acaccatccg tccgccgctg cagctgatca aaactgcacc
    27901 gggcagctcc ctggtatctt ccgtgttctt catgctgctg ccggaacagg tttacgttta
    27961 cggtgactgt gcgatcaacc cggatccgac cgctgaacag ctggcagaaa tcgcgattca
    28021 gtccgctgat tccgctgcgg ccttcggtat cgaaccgcgc gttgctatgc tctcctactc
    28081 caccggtact tctggtgcag gtagcgacgt agaaaaagtt cgcgaagcaa ctcgtctggc
    28141 gcaggaaaaa cgtcctgacc tgatgatcga cggtccgctg cagtacgacg ctgcggtaat
    28201 ggctgacgtt gcgaaatcca aagcgccgaa ctctccggtt gcaggtcgcg ctaccgtgtt
    28261 catcttcccg gatctgaaca ccggtaacac cacctacaaa gcggtacagc gttctgccga
    28321 cctgatctcc atcgggccga tgctgcaggg tatgcgcaaa ccggttaacg acctgtcccg
    28381 tggcgcactg gttgacgata tcgtctacac catcgcgctg actgcgattc agtctgcaca
    28441 gcagcagtaa tctcgtcatc atccacagct ttgcgctgcg gatatctgaa ccggaaataa
    28501 tcactatttc cggtttttta ttctcttaat ttgcattaat cctttctgat tatcttgctt
    28561 aactgcgctg catcaatgaa ttgcgccatc tcactttgca tacttaccac tttgttttgt
    28621 gcaagggaat atttgcgcta tgtccgcaat cactgaatcc aaaccaacaa gaagatgggc
    28681 aatgcccgat acgttggtga ttatcttttt tgttgctatt ttaaccagtc ttgccacctg
    28741 ggtagttccg gtggggatgt ttgacagtca ggaagtgcag tatcaggttg atggtcaaac
    28801 aaaaacacgc aaagtcgtag atccacactc atttcgcatt ctgactaacg aagcaggcga
    28861 acctgagtat caccgcgtac agctgttcac gacgggcgat gaacgcccgg gcctgatgaa
    28921 cttcccgttt gaagggttaa cctcaggatc gaaatacggg acagccgttg gcatcatcat
    28981 gtttatgctg gtgattggcg gcgcgtttgg cattgtgatg cgtacaggaa ccattgataa
    29041 cggtatcctg gcgcttattc gccatacccg cgggaatgaa attctcttta ttcctgcgct
    29101 gtttattctg ttttcacttg gcggcgcggt atttggtatg ggagaagagg ccgtcgcctt
    29161 tgccattatc atcgcaccgc taatggtccg gctgggctat gacagtatta ccaccgtcct
    29221 ggtgacttat attgccacgc aaatcggttt tgccagttcg tggatgaacc cgttttgtgt
    29281 ggttgttgct caggggattg ccggcgttcc ggtgctttct ggctccgggt tgcgcatcgt
    29341 ggtgtgggtt atcgccactc tgattggcct gatctttacc atggtgtacg cctcacgagt
    29401 gaaaaagaat cctcttctgt cacgcgtgca tgagtccgac cgcttctttc gtgaaaagca
    29461 ggcggatgtt gaacaacgtc cgtttacctt tggtgactgg ctggtattga ttgtcctgac
    29521 agccgtaatg gtctgggtga tttggggcgt gatcgttaat gcctggttta ttccagaaat
    29581 tgccagccag ttcttcacca tgggtctggt gattggcatc atcggcgttg ttttccgcct
    29641 taacggcatg acggttaata ccatggcttc atcctttacc gaaggggcgc gaatgatgat
    29701 cgcccctgcc ctgctggtgg gtttcgccaa agggattttg ctgctggttg gtaatggtga
    29761 agcaggtaat gccagcgtgt taaatactat cctcaacagc attgccaatg ccattagcgg
    29821 tctggacaac gcggtcgcgg cctggtttat gttgctcttc caggctgtat ttaatttctt
    29881 cgtgacgtcc ggttctggtc aggcggcatt aactatgccg ttactggcac cgcttggcga
    29941 tctggtcggt gttaaccgtc aggttaccgt gctggcgttc cagtttggtg atggcttcag
    30001 ccacatcatt tacccaacct cagcttcgtt aatggcaacg ctcggtgtgt gccgggtgga
    30061 cttccgtaac tggctgaagg tgggcgcgac cctgcttgga ctgctgttta ttatgtccag
    30121 cgtcgtagtg atcggcgctc agttgatggg ctaccactaa aaatgttaag agccgcaatt
    30181 gcggctcttt tttcattctg ccgtttcagt ctctacggct tcatttttgg cattgcgctt
    30241 catccacaac gccagcgctt tcagcgagtc tggagtgaac tcatcgcagc gtgcggtgat
    30301 ttcttccggc gtcagccagc aaactccact gacttcatct tcctgtaggg cgaagggacc
    30361 gtgagagacg caactgaaca acgcgcccca gacacggcaa tttttatctt cgaaatagaa
    30421 ctgcccgtgc tcggcaaagg ggacaccggc aatgcccaac tcttcttccg cttcgcgacg
    30481 cgcggattcc agcagttgct catcggcctg aactacaccg cctgcggtcg catctaacat
    30541 gccgggtaaa aagtcttttg tctcggtacg acgctggacc agaattttgc ccatgccatc
    30601 atgcacgacg atgtaagttg cacgatgacg cagacactgt gcccgcattt gttcccggct
    30661 ggcttgtgca atgacttcgt tctcttcatt gacaatatcc acccattcag tacttgccaa
    30721 acgacgctgt tccattatca ggaaaccttc tttttctggc gctcttacgg cgcattttga
    30781 gttgtgggta aattacggat taatcgcgac ctgcgcaatg atactttgat cattgagtgc
    30841 gattacacta agtacgtcat tatccagcat gccataaccc gccggattac cgcctttcgg
    30901 aatactcacc gagccggggt tgaagtggaa aatctccccc cgctgttctg ccactggcag
    30961 atgggtatga ccgtacacca gcacatcgtt ctggtttaaa gcaggtagat tttccgggcc
    31021 aaaaagatga ccatgcgtca aaaacagacg ttgtttttcc agtaatacct gttgccacgg
    31081 cgcggttatc gggaaatgca gcatcatttg atccacttcg ctgtcgcagt tgccacgcac
    31141 agcgataacc ttatgtgcca cttcattaag ccgttcagcg actttggctg gcgcgtaacc
    31201 ctccggtaaa gcattacgcg ggccgtgatt caacacgtcg ccaaggatca ccagccactg
    31261 ggcaccgctt tggacaaaca actccacaac acgttccgtc gccggtaacg acccatgaat
    31321 gtccgatgca aacatcagtt tcatcactca ctcctcgtcg aaaaaacgta agctatgata
    31381 ctggattctg tcgttgctat cagccagatt gcttcgccga aagtgcaata aaacgctgga
    31441 cagacgctcg ctgccactgg aatgtcgcat aatccaccag gcgttctggc acctcatcgc
    31501 catgtagtac caggcggtta atcattagcg ccagatcagt atcagcaatg caccattcac
    31561 caaataaatt cggctgaccc agtgctaaca aatgttctgc catcgcgaac agtttctctg
    31621 cactggcttt tccctcggcc gttagtggcg cttttttcgc ccccgcaaag acaacatccg
    31681 tcggacgctc ttcgcggatg ggcatcagat cgctgcgcag ccaggcctga atctgtcgtg
    31741 cacgcgcacg attttctaaa tcaagcggat aaatacgttc ccaggtcggt ggcgcaaatc
    31801 gatcttccag atactccgca atggcagaag attcactcaa ctcaaaatca tcgatttgta
    31861 ataacggcac acggcgtgtc tgaccgtaac cttgccacgt cggctgcaaa tgttcaccgc
    31921 tgtcgaggtc gatggtcttg atatgaaacg acaggccttt ttcctgcaac gccacccagg
    31981 cggataacac ataaggggag aaaaagtggg catctgacca aagcgtgata gcgggtttac
    32041 tcattatgtc ctcggcaata aagggggctg gctatcaaac tatagagcct ttttgtcact
    32101 gtcacgtgac gaatactcac gtaagtcgcc gcgctttcta tacttgtggt gttcagatga
    32161 ttaaacaacc ggagctgcaa tgatcgatct ctatttcgcc ccgacaccca atggtcacaa
    32221 aattacgctg tttctcgaag aagcagagct tgattatcgc ttgattaagg tagacctggg
    32281 gaaaggcggt cagtttcgtc cggaattttt gcgcatttcg cccaacaaca aaattccggc
    32341 aattgttgat cattctcctg ccgatggcgg cgaaccgtta agcctctttg agtctggtgc
    32401 cattttgttg tatctggctg agaaaacagg actctttttg agtcatgaaa cgcgtgagcg
    32461 cgccgccaca ttacagtggt tattctggca ggtaggcgga ctggggccga tgcttgggca
    32521 aaatcatcat tttaatcacg cagcccccca aaccattcct tacgctattg aacgttatca
    32581 ggttgaaact cagcgtcttt accatgtact aaacaagcgg ctggaaaact cgccctggct
    32641 gggaggcgag aactacagca ttgcggatat tgcctgctgg ccgtgggtta atgcctggac
    32701 tcgcctgcga attgacctcg caatgtatcc ggcagtcaag aactggcatg agcggatccg
    32761 ttcgcgccct gccaccgggc aggcactgct aaaagcacaa ctcggtgatg agcgttcgga
    32821 tagttaacag aaacaggttc tcgtgtatta tttcatccta agtaaaacaa cggagaacct
    32881 gcaatggcac aacctgccgc tattattcgt ataaagaacc ttcgtttgcg tacgtttatc
    32941 ggaattaagg aagaagaaat taacaaccgt caggatattg ttatcaatgt gacgatccac
    33001 taccccgccg ataaagcgcg cactagcgaa gatatcaacg atgcgctgaa ttatcgcacc
    33061 gtaacgaaaa acattattca gcatgtagag aataaccgtt tctctttgct ggaaaaatta
    33121 actcaggatg tgctcgatat cgcacgtgaa catcactggg tgacgtatgc tgaagtggag
    33181 atcgataaac tgcacgcgct gcgctacgcc gattcggtat ccatgacctt aagctggcag
    33241 cgttaatcgc catatcggga ggctgcatga acatagtgat caccggaggg acgggattaa
    33301 ttggtcgcca tttgattccg cgtttgctgg agctgggcca tcaaattacg gtagtgacgc
    33361 gtaacccgca gaaagccagt tccgttctcg gccctcgggt gacactatgg caagggcttg
    33421 ccgatcaaag caacctcaac ggcgttgatg cggtaatcaa cctggccgga gaaccgattg
    33481 ctgataaacg ctggactcac aagcaaaaag agcgtctctg ccaaagccgc tggaatatca
    33541 cgcaaaaact ggtcgatttg attaatgcta gcgacacgcc gccgtcggta ctcatttccg
    33601 gctcggcaac gggctattat ggcgacttag gtgaagtggt ggttaccgaa gaggaaccgc
    33661 cgcataacga atttactcat aaactctgcg cccgctggga agagattgcc tgccgggcgc
    33721 aaagtgacaa aacgcgagtg tgcctgctgc gaaccggcgt ggtactggca ccggacggcg
    33781 gtattctcgg taaaatgctg ccaccgtttc gtcttggcct gggcgggccg attggttccg
    33841 gtcggcagta tctggcctgg attcatattg atgatatggt caacggcatt ctctggctgc
    33901 tggataacga gctgcgcggg ccatttaata tggtttcgcc ctacccggta cgcaatgaac
    33961 aatttgccca tgcgctcggt catgcgctgc atcgcccggc tattttgcgc gtccccacaa
    34021 ccgccattcg gctgttaatg ggcgaatctt cagtactggt attaggtgga caacgcgcgc
    34081 tgcctaaacg gctggaagaa gcgggttttg cgtttcgctg gtacgattta gaagaggcgc
    34141 tggcggatgt cgttcgctga tgtggtttac agcaaacatc cgccagttaa ctccctgtgt
    34201 tacaggatta gtggctttgc gcgataagat cgtctggtga aagtcgggtc accatcataa
    34261 ctaactctct gtctaaacct ctatccagca tctcctgagc aatacgcagg gcttcttcgt
    34321 gtttgccctg cattgcgcct tcttcacgta atctgtcagc aatggtcatc agtttctcct
    34381 tttcttgtgg tgcgcgttct gttatctcac caataaatgc acggaaacgc tgggcatccc
    34441 ctgtttgtaa tacgtaatta aacagggctt ttagctgtct gtcattagtg ttccctgtaa
    34501 ctagcagcga aacaatttgg tcgactaatc ccaacagatc gcgctgacga atatgtttct
    34561 gaattaactc caacagcgcc attttgcggt gttgcataat ctcgtcatcc ggcaccacgg
    34621 taatatccac caacggaaac gctgatgaat atattttgcg ggctatagca ggctcggcaa
    34681 attcatcaag ccagcagagt gaataaggat aaggacttct gcaaccatga taaaacagca
    34741 tcgggatcac caatggaagc tctttatagc ccgcatcaag atggttttgc attgccgcaa
    34801 tggaataacg catcatgcga aaagccatta attcttccgg cttactttgg tgctctatca
    34861 ctacataaat ataacccact ccctcctgcg ttttcacaga ccacaagagg tcggaataat
    34921 attgccgcag gtcttcatca ataaaactgt ttggttctag tttaagcgtc gttaaatcac
    34981 acagtttgcg cagcggcgcg ggaagatgaa tattaataaa attccgcgcg gtgtctggat
    35041 ggcgtaaaaa agatttaaat accgcatcat gcggcgtgga agttgtcgat attgtcatgg
    35101 cgttccgtca cccctcaaat ttaagatgac gcgacaataa ccacgattaa cacccaatgc
    35161 agcaggcagt ttttatcttt ggacagcgaa ttcagaagta tttttagcgg actgcaactt
    35221 ttacatttca gcggaagctg actcgcaaat tccccttgaa aatactccgg cagcgttagc
    35281 caccggagag gaaattattt cagcgatccc ttaaggaact gttgcagacg agggctttgc
    35341 gggttgccaa ataactgctc cggcgcgccc tcttcttcta ttttcccctg atggaggaaa
    35401 atgacgtgag tagaaacatg gcgagcaaag cccatttcgt gagtcactac caccatggtt
    35461 ttcccctctt ctgccagttg ctgcataata cgtaacactt cgcctaccag ttcaggatcg
    35521 agcgccgagg taggttcatc aaacagtaaa acttctggtt ccatcgccag cgcccgcgcg
    35581 atagaaacac gctgttgctg accgccggaa agatgcaccg gatatttccc ctgcgcacgt
    35641 tcgtctatcc cgactttcgc cagatacttc accgcccgct cgcgcgcttc ctgcttgctc
    35701 aggcccaaca cctgaatcgg cgcttccatg acgttttcca acaccgtcat atggctccag
    35761 agattgaagt gctggaatac catcgtcagg cgtgtgcgca gtaagcgcag ttgattttta
    35821 tcggcgactt tgagttgacc gtctttgtcg cgcaccagat tgatcgtctg gccattgacc
    35881 acgatcgatc cttcactcgg tttttcgagg aagttaatgc agcgcagaaa ggtacttttc
    35941 cccgatcccg acgatccgat gatgcttatt acatctccgg cattcgcttg cagtgagacc
    36001 cctttcagca cttcgtgttc gccatagcgt ttgtgcaaat cgataacgtt taatttattc
    36061 tcggacatcg tattctcagt gcgttgaaga aggtttcaca tgctgcaacc agcgtttttc
    36121 cgctctgcga aagaggctga tcaggacata agagatgatt aaatagagca ccgcggcaat
    36181 gccgaaggcg gtaaaaggtt gatacgtggc ggcgttaata tcgcgggcta ttttcagcag
    36241 atccggcaca gtggccgtaa acgccagcgc agtagagtgc agcatcagga tcacttcgtt
    36301 gctgtatgcc ggtaacgcaa tacgcagcgc agaaggcaaa ataatgcagc gatacatttt
    36361 aaaggtcgag aagccatagg ctctggcggc ttcaatttcc ccatgcggaa ccgaacggat
    36421 tgccccagca aaaatctcgg tagtgtaagc gcaggtgtta agcgtcagcg ccagcacggt
    36481 acagttcagg ccactgcgga agaaagcgtt aaggaattcg gttcccttaa caatctcaag
    36541 cgtgtacatg ccggaataga acaccagcaa ctgaacatac agcggcgtac cgcgaaaaat
    36601 ataggtaaat aaccagattg gaaactggat gtatttatta ctggagacac gaccaatcgc
    36661 cagaaacagc gccaggactc cgcctatcac taccgacaaa ataagcagcc acagagtgat
    36721 cgccacacca gtaaagcgat aaccgtcggt ccacagcagc ggtttccagt attcatgtaa
    36781 gatttcgatc acaggtcagc cctcttcaca cccacggagt agcggcgctc aaggaacagc
    36841 agcacaccat tggaaacggt ggtgaaaacc aggtaaatca cgccacagac gatggcgaaa
    36901 tagaacggtt cccaggtact tttgcctgcc agctgcgtgg ctttgaccac atcttccagg
    36961 ccgagtaacg aaaccagtgc ggtagatttg aggatcacct gccagttgtt gccaatgcct
    37021 ggcagggcgt aacgcatcat cgccggaaac atgatccgcc gaaacacttg cccgcgagta
    37081 aaaccgaacg ccgtcgccgc ctctatatgc cctttcggca ctgccataaa cgcgccacgg
    37141 aacgtttcgg taaaataagc accgtagata aaaccgagag tgataatacc agcgaccatc
    37201 ggatcgatat caatctgccc gacgcccatc gcctccgtca ccatgtttag cgcaatctgc
    37261 aaaccataga aaatcagcag catcaacacc aagtccggca cgcctcgaat cagcgtggtg
    37321 tacccttcga aaatcagccc cgaaagccga ttttgcgaga gcttaccgcc agcgccaatt
    37381 aaaccgatga ttacagcgag cactacagag ctgatagcca gctccagcgt gacgagcgca
    37441 ccctgtaaaa taacacctga aaacccatac aacatgctgc ctgtcctgtc gtgtgtggtg
    37501 aattaccgcc tcttgtctcc ctccggttac ccgaagggag cgggctttct ttcaccgatg
    37561 gcgattaacc accataaaca tcaaaatcga agtacttttt cgctaatttc tcgtaagtac
    37621 cgtcagcgcg catttcggca aaggctttgt tcagtgcctc gcgcagttcg ttatcttctt
    37681 tacgcaggcc catgccggtc cctacgccaa acagtttttc atctttaaca gacggaccac
    37741 cgaatttgta atctttaccg acgggttgtt tgaggaaacc ttcgctggca gcgacctcat
    37801 cctggaacgc ggcatcaata cgtccggcag tcaggtcaga ataaatgttg tcctgcccct
    37861 gatacgagac gatttcaatg ccttttggtg cccagtgttc attaccgaac gtctcctggg
    37921 tggtgccctg caatacgcct acccgtttgc ctttcagcga ctcgactgtc ggctgaatgt
    37981 cagaattttt cgccaccacc agacgagaat cggcagcgta cagtttgtcg gtgaaggcaa
    38041 tttcttgctg acgtttttcc gtaatggaaa gcgatgacat gatggcgtca atcttcttcg
    38101 cttttaagga cgggattaac gcatccagcg ggttttcgac aaacgtacat tgcgtattga
    38161 tgcgcttgca caactccttc gccagctcga tatcgaaacc aaccagttcg ccttgtgaat
    38221 tttttgattc aaatggcgca taggtcgggt cggtaccgat gcggatgttt tgcggaattg
    38281 cagcaagtgc cgcagttgcg ctggagaagg ccagaaccag agagagcgat agcaccagtt
    38341 ttttcataat gtcctcaact gacagtcttt ttatagggaa tatttacagg tttgttacac
    38401 ttatcgtgcc ataaaattga ccatcaaggc aaaatatccc gccccgttag acgtattatt
    38461 tttcattaaa aatgcttaag tatgtatttc aatgcaccac gatagtgcaa caaacctggc
    38521 gtgcaccatg atgatcattc catcaggtac agcttcccag cgacgtatca gtcaccgtag
    38581 acattaaagt cgaaatactt tttcgccatc ttgtcgtagg tgccgtcctg acgcagctcg
    38641 ccaagcgcct tattgaaggc agccgtcagt tcagcatcat ctttacgtag ccctacaccg
    38701 gtgccatcac cgaagtattt tttgtctttt actgatgagc cagcaaaagc gaaatcttta
    38761 ccagcaggtt gcttgaggaa tccttcgctg gcagcaactt catcttgtaa cgcagcatcc
    38821 agacgtcctg cagccagatc ggaatagacc aaatcctggt tggcataggc caccacatcc
    38881 acgcctttac tacgccaggt ctcgttagcg taagcttcct gggttgatcc ctgcagcaca
    38941 ccaacatgtt tacctttcag tgaatccagc gttggctgaa tcggtgaacc tttggcagca
    39001 atcaaacgag aatctgcggc gtacagcttg tcggagaagg caatctcctg ctgacgttta
    39061 tcggtaatgg aaagcgacga aataatagcg tcgatttttt tcgctttcag tgaggggatc
    39121 agcgcgtcaa agtcactggc aacccaggta catttcacct gcatccgttt gcacatctcg
    39181 ttaccgagat cgatatcaaa gccaacaaaa tcacctttag catctttcga cgagaacagc
    39241 gcataggtgg tatcggttcc gatacgtacc gtctccggta gcgccgcata gctggaagcc
    39301 gctgcggaga gaccgactaa taaagacaga gcgagaatcg acttcttcat acataaccct
    39361 caagtgaaaa tggctttttt atgttgtgta ttgtgttgtg tgtttgcagg ctctttcatg
    39421 caggtcttat gccatcttga cgacaacagt aacattcaac gttaaatatg ttaataagac
    39481 gttgcattat tgtcctgaag ttgaagatag caggtatagc ggttgaatcg cagcgtttcg
    39541 attgaatggc agaaacaaaa tgtcgaggat ttgatcgcgg ttgcaaaatt gccctgaaac
    39601 agggcaacag cggagttatg cgccctgcca gcgggcaaag agatcttcag gaagggttat
    39661 tgcaaactgg tcaagaacac gattaaccgt ctgatttatc acatcatcaa gggattgcgg
    39721 gcgatgataa aacgccggaa cgggaggcat aatcaccgca ccgatttctg ccgcctgagt
    39781 cattaaacgc agatggccta agtgcaatgg tgtttcacgc acgcagagca ccaacgggcg
    39841 acgctctttc agcaccacat ctgccgcacg ggtcagtaag ccatcagtat agctatggac
    39901 aatgccggac agggttttga ttgaacaggg taaaatcacc atccccagcg tctggaaaga
    39961 accggaagag atgctggcgg aaatatcgcg cgcatcgagc gtgacatcag ctaatgcctg
    40021 cacttcgcgc agggaaaaat ccgtttcgag ggataaggtc tggcgcgccg cctggctcat
    40081 caccagatgc gtttcgatat ctgtgacatc gcgcagaacc cgtaataagc gcacgccata
    40141 aatcgcgccg ctggcaccgc tgatgcctac aatgagtcgt ttcataaata gttgccctgt
    40201 cagacttgca ggcagacttt gcagtatttc ggcgggagtt gcaagtcagg gtgccagacc
    40261 ggcaccctca gcgaaggcat catccttcgt tatgcatttc gagattttcc acttcgttct
    40321 gacgttgcac tgctttggcg tcatcattac gtaacgtatc gaggaaatcg aggtagccct
    40381 gatcaacatc tttggtgacg tagacgccgt tgaacaccga gcattcaaac tgctggatat
    40441 ccggattttc agcgcgaacg gcgtcgatca gatcgttcag atcctggaaa atcaacccgt
    40501 cagcaccgat gatctggcga atttcatcaa cttcgcgacc gtgagcgatc agttccgtgg
    40561 cgctcggcat atcaatacca taaacgttcg ggaagcgaat ttccggtgcc gcagaagcga
    40621 ggtacacttt cttcgctccg gcttcgcgtg ccatctcgat aatctgctca gaagtggtgc
    40681 cacggacgat ggagtcgtcg accagcagga cgtttttatc gcggaactcg gcgcggttgg
    40741 cattcagttt acggcgcacg gacttacgac gcagctgctg gcccggcatg ataaaggtgc
    40801 ggccaacata gcggttttta acgaagccct ggcggtacgg tttgcccaga atacgagcaa
    40861 tttccagcgc gatatcacac gaggtttctg ggatcgggat caccacgtcg atatccagat
    40921 cttcccattc gcgggcaatt ttctcgccca gtttcgtgcc catattcaca cgcgcgctgt
    40981 aaacggaaat tttgtcgata aacgagtccg ggcgggcaaa gtatacatac tcaaacaggc
    41041 acggattgct gaccggattg tcagcacatt gacgggtaaa caactgccct tcttcagtga
    41101 tgtaaatcgc ttcgcccggc gcgacgtcac gcaggaaatc aaagcccagc gtatcgagcg
    41161 ctacgctttc ggaagcgacc atatattctg tacggttctc gtcaatatca cgttttccca
    41221 gtaccagcgg acgaatcccg tttggatcgc ggaaagcaac cataccgtgg ccgataatca
    41281 tcgccacaca ggcatacgcg ccgcggatta agcggtttgt ggcagcaatg gcagcgaaaa
    41341 tattgtcggc ttccagcggg tagtggcgga agttgtccag ctcgctggcg aagatattaa
    41401 gcagaatttc cgagtcggaa gtggtgttga tgtggcggcg tttttcttca aacagttttt
    41461 tacgcaactc gtgagcgttg gtcagattgc cgttgtgggc aagcgtaatg ccatacgggg
    41521 agttaacgta aaacggctgc gcttcagagg cgctggagct gccagccgta gggtaacgca
    41581 catgaccaat gcccatattg ccctgcaaac gctgcatatg gcgagcttca aatacatcgc
    41641 tcaccagccc gttcgcttta cgcaaacgga agcagttatt ggcatctatg gtgatgatgc
    41701 cggcggcatc ctgaccgcga tgctgaagca ccgttaaggc atcataaatc gactggttaa
    41761 ccggcataac accggcgata ccgacaatac cgcacatacg tctttttcct cgttaagcca
    41821 catctcagag cacttacgct ctgggcaaga aacttgacga gctttgcaga taatcaaaaa
    41881 accatctgat gataaaactg aattgcggga tcagctgtga tttgctccag tcttcacttt
    41941 tcgacacccc ggtaaaggag tcgagaaaga agagaatggc ggcaacaatc aacacaccgc
    42001 gcaacgcacc gaaacagacg cccagcaccc gatcggtgcc tgacaacccc gttttctcca
    42061 ccaactggcc tatcacgaag ttcacgatag caccaacgat cagggtagcg ataaacagta
    42121 ccgcgatggc aatcccattt cgaaccagtt cgtcttcaaa gcccgtaaac cagactgaca
    42181 ggtaagtgta gtaatgactg gcaacaaaga aagcacaacc ccatgtcacc agcgataacg
    42241 cttcacgaac aaagccgtgg atcaggctaa ccagagagga aaaagcaatc accgcgatta
    42301 tggcgtaatc aatccagacc atatgtgtcc cacgatttta cgccctgtca tcctgttcgg
    42361 ggcgcattct aacagaaaaa gaaaacgttt gcgtagggat ttccttcccg cgcatcaata
    42421 aaaatggcgc tgaaaaaata ttcaacgcca tcgacttttt atgcctttgc ggcatcgtgc
    42481 aatgcgtgtc ggatgcggcg taaacgcctt atccgaccta cggttctaac actgcccaag
    42541 cctgataaga cgcgccagcg tcgcatcagg caagactgta ttaattcggc gtatagccca
    42601 ttaccacgcc acttaagcca gaaagttgct tcaactcacc cagcgaacct ttcagcttat
    42661 ctttcgaggc atccggccca accagaatac gggtaatttt accctgcact ggcgtggatg
    42721 gcgacgtata aacccgataa ccggcaccgc gcagcttacc gacaatctca ttcactttat
    42781 cggcattttt cagcgcaccc agttgcacaa cataggcttt acccgttggc gctgcttttt
    42841 cttccacgac cggcttcggt tctggcttcg gcgcaggtgg tgcttcaacc tttggtttag
    42901 gcggctccac cggtttcggc ttcggtgggg cgaccggtgc aggttccggt tcaaactcgg
    42961 tgttattggc tgcaatagtg gccggatcga gcgacggtgc tgcggcatca cctgcccgca
    43021 cctcttccgc tgcgccttcc ggcggctgcg tcggcaacgc ctgggtggcg gctggcatca
    43081 tatcaggctc atcacgatcg cccgctttcg gcaccagcgg gatagccgcg aactcatcct
    43141 gataatgttt tttctgcccg tccagcagcc ctggaagtac aatcaccccc agcgccacca
    43201 gcacgatcgt gcccactaac cgattctgaa acttacttgc caccgcttct cctcgcgtca
    43261 atcacttcca tgacatgtgc gaccgtgtgg aaagaaccac acaccagcac ggtgtcttcc
    43321 gctttagcgt ccgccattgc ggcatcccat gcctgcgcaa cgctatcaaa tgatttgccg
    43381 ttacccaaat gctcaagcag ttgttctgcc gtggcaccgc gcggcccttc cagtggcgca
    43441 caataccagt catcaaccac gcttttcaac caggccagag ttccggcaat atctttatca
    43501 tgtagcatac cgataaccgc cagcacgcgc ccgtttttcg gtagcgcttt catacgcccg
    43561 gtgagatatt ccgccgcatg tggattatgc gcgacatcaa aaataacgcg tggcgactcg
    43621 ctcacaatct ggaaacgtcc cggcaaaatt gcgctggcaa tcccgtcgcg aatggcattt
    43681 tcactgactt ccagcccgct ggcacgcagt gccgccagcg ccgttgcggc attcggttgc
    43741 gggacaagcg gcaacggcag attttccagc gtgccgtgag catcgctaaa cgcccagtca
    43801 tgatcggtga cggaatagtt ccactcaacg ccccgacgtt gtaacagtgc acctttttcc
    43861 tgcgccacat cagcaatggt agaaggcatt tccggctcac cgacaattgc cggtttttcg
    43921 ctgcggaaga tgcctgcttt ctcgcgacca atactttcgc gatctggacc cagccagtcg
    43981 gtatgatcca gcgcaatact ggttactacc gcgacatcgg catcgacaat attggttgcg
    44041 tccagacgac cgcccagccc tacttccaga atcaccacgt caagttgtgc ctgcttgaac
    44101 agccacaacg ccgacagcgt accgtactcg aaataggtca gggaaatatc accgcgtgcc
    44161 gattcaatct ccgcaaaaga ggcggtgtgg gccgattccg gcaattcctg gccctgcaca
    44221 cgtacgcgct cggtataacg caccagatga ggcgaactgt agacgcccac tttgtaccct
    44281 gccgccatca gaatcgactc cagcgtacgg caggtggtgc ctttgccatt cgtacccgca
    44341 acggtaaaca caaatggcgc tggtttcagg acgccaagac gcgccgcgac ctggctcacg
    44401 cgctcaaggc cgagatcgat agttttactg tgcaggtttt ccagataaga aagccacgaa
    44461 gccagaggcg acgcggcttg aggagtgcgt ttgataatca tggtatccgc tgattcgtta
    44521 cggtgagaat agcaaaaggg cagagccagt ggccctgccc ttatcagtta tcaggcctca
    44581 ggttcctgat ccggtaccgg gggtaccact acgccttcac gcggcgcttc aggattcggc
    44641 gctggcagat tcatcaactt cgccagaatg ctcgccagtt tcaggcgcat ttccggacga
    44701 cggacgatca tgtcgatcgc gcctttctcg atcaggaatt cactgcgctg gaatctaggc
    44761 ggcagttttt cgcgaacggt ctgttcgata acacgcggac cggcaaagcc gattaacgct
    44821 ttaggttcag cgatgttgag atcgcccagc atggcgaagc ttgcagaaac accgcccatc
    44881 gtcgggtcgg tcagcacgga gatgtacggc aagccgcgct cctgcatttt tgccagtgcc
    44941 gcagaggttt tcgccatctg catcagcgac atcagtgctt cctgcatacg tgcgccacca
    45001 gaggcggaga agcagatcag cgggcagtta tcttcctgcg cctgctcaac ggcacgcacg
    45061 aaacgtgcac ccacaacaga ccccattgaa ccgcccataa aggcgaactc gaatgccgca
    45121 gcgacaaccg gcattccata cagagtgcct ttcatcacca ccagcgcatc tttttcgccg
    45181 gtttctttct gcgcagatgc cagacggtct ttatacttct tggagtcacg aaacttcagc
    45241 tcatctttcg gctcaagctc gctacccagc tccacaaggc ttccttcatc taacaggcta
    45301 tgcaggcgat tacgcgctgt catacgcatg tgatggtcac acttcggaca gacctcaaga
    45361 ttacgttcca gctcagcgcg gtataaaacc tgaccgcagc tatcacactt agtccacacc
    45421 ccttcaggaa tgctcgcctt gcgggtggga gtaatgttgc ttttaattcg ttcaatccag
    45481 ctcattaggg acctttctgt ctgaacctgg ttcgatgcca gttttatctt tggggacgca
    45541 taatgccatt tttgccccca acagaccatg aatgttgcac attaaaacat aacagcccga
    45601 aactttggat aaaaaagtgg tcgaaccgcg gagttacttt ttattttgcg gcgcgtgccg
    45661 cagcgcgttt gtgacggatt atttcgatga cgcccggcaa aatagaaacc acaataatcc
    45721 cgacgatcag cagcttaagg ttatcctgaa ccatcgggat tgtaccgaag aaatagcctg
    45781 cgtaggtaaa aagcagtacc cacaacagtg cgccgatcac gttataagca gcgaaatgac
    45841 ggtacgacat gtggcccatt cccgcaacaa acggggcgaa cgttctgacg atcggcacaa
    45901 aacgggcgag aataatcgtt ttgccgccat gtttctcata aaactgatgg gttttgtcga
    45961 gataactgcg acggaaaatt ttcgaattgg gattactgaa taacttttca ccgaacaacc
    46021 gcccgatagt gtaattgacc gcgtcaccca caatcgcggc gatcagcatc agcactacca
    46081 tcatatggac gttgagatcg ttggtttcca gtgatgccaa cgctccggca acaaacagca
    46141 atgaatcacc cggtaaaaac ggcattacca ccagaccggt ttcacagaac aaaattaaga
    46201 acaaaatggc ataaacccag acgccgtact ccgcgactag ctctgccaga tgcacgtcaa
    46261 tgtgcaggat aaaatcgatg aggaaataaa tcaggtccat atttgcctat gccttgtact
    46321 cgtcatcttt caggctgtaa ctacgttggc tacagcctga aatgttccga gcattattct
    46381 cgttagtccg ccagaaatag cgggcccatt ggcggttttg gaagatcata ccggtcaggg
    46441 taatccaccg cgaccagata cagcccttcc gcttttgccg ttgctgccgc cagcgttctg
    46501 tcctttgccg ccagcaactc tgctatccag ctctccggct ggttgtgggc accgacttcc
    46561 atcaggctgc cgacaatatt cctgaccata tgatgtacaa aggcattcgc tttgatatca
    46621 accaccacat aaggaccgtg acgcgtgaca ttaatatgca taacgttgcg ccacggggtt
    46681 cgggactggc actgcaccgc acggaacgag gtgaaatcat tctcgcccag caagcattgc
    46741 gcagcccgat gcatccgttc agcgtccagc ggttcgtaaa aatgggttac ccctttactc
    46801 agtaccgccg ggcgcagccg atgattgtag atgatgtagc gataacggcg agccgtggcg
    46861 ctaaatcgtg catgaaaatc atcaggtaca gctttaaccc aacgcacggc gatgtcacca
    46921 ggtaaattcg catttacgcc caaggtccac gccgcgtctt tgcgctgcgc ggttgtttcg
    46981 aaatgcacca cttgcccggt accgtgtacc cctgcgtcag tacgcccggc gcagaagacg
    47041 gtgatgggtt cgttcgccac ctgggagagc gccttttcca gcttctcctg cacactgcgg
    47101 acttcattct gccgttgcca gccgtaatac ttactgccgt cgtactcaat gcccagcgca
    47161 attttataaa ctggcagttg ttgctggtcg gacattagta cagatactcc tgcaccagtt
    47221 tctcggcgat ttttactgcc atcagcgcgc cgccaaagcg aacgttatcg gccaccgacc
    47281 agaactggac ttgctccggc ataccgtagt cattacgcac gcagccaaca gaaagatgcg
    47341 gcgtacccga agcatcccct acctgagtcg ggaattcgtt ctcttcagag agcacaatat
    47401 cttcgccctg agcaaacgca tcacgcgctt cttctgccgc cagcggacgc agtgcttcaa
    47461 agttgaccat ctgggcatga ccgtagaata ccggtgcctg gacgacgctt gccgaaatca
    47521 tcagcccttc gtcctgcagg attttgcgta cttcgtcaac gatacgacgt tcttcacgca
    47581 cgctaccttc gctatccggc aacaacggca gcatattgaa cgccagttga cgaccgaaga
    47641 aatcttcttc gtcaatcgga atgccgttga gcaatttcgc actctgcccc gctaacgcat
    47701 cgaccgcttt tttgccctgg gcggaggctg aaatcaggct ggtaacactg atacgcgaca
    47761 aaccgccctg atcgattaac ggtttcagtg ccgccagcag ctggctggtc agactgtctg
    47821 gtacggcgat gacattccgg ttgcggtaat cggtcagtac aaacgggttt acttccggca
    47881 ccaccagcgg tacgtcgggt tcgagagcaa acagtccgct gctgtcgatc accaggcaac
    47941 ctgagttggt cgcttcttca acccaggcag cggtagcttc tttgcctgcg acaaaaaatg
    48001 ccagctgcgc ctgcgtccag tcgaattcag cggcatcctg cacggtgatt gttttaccac
    48061 caaagcgcag ttgttcgcct gcgctttcgt tacgtgccag tgcataaatt tccccaaccg
    48121 ggaactgacg ttcagccagc gtttcaagca gggcttcgcc catagcgcca gttgcgccca
    48181 ggacggcaat gttccagcct tcagacatgg tggtttactc cagaaatagc aaagctccct
    48241 gccaatgtta cagcagagag catgaagaag agattaacgt gccggatgat gaacggcgtt
    48301 aaaacccagt ttacacagca atgatgccgc actggcgtca tcacaaatta catacagaga
    48361 tgaccattca cggcgctcaa gatagttttt gcgcagttta tcgaactcac ccggtatccc
    48421 ggcgacttta cgcagcggtg cgtcatcgcg gcgcacatca tacaccaaat gcaccagcct
    48481 tttcagcgtc ggttgatcga gcggaccatg cagcgtaatg cggccaaact caggcgcggg
    48541 cagcaacgta tccagcgcaa cgtgttgttc atgcccaata aacttgctat aggcttcaaa
    48601 cacttgcgtc gtgccacgcg ctttaccctc cagggtatag cctgcgatat gcggcgtgcc
    48661 gatatccact tttgtcagca attccacatt aagttccggc tcgccttccc agacatccag
    48721 caccacgctt aacttctggc cttcattcag gcaggtcagc agcgcggtat tatcgacgac
    48781 tgcgccacgg caggcgttaa tcagaatcgc tccgggcttc aggctacgga tcagtttttc
    48841 atccgccaga tgtagcgttt tgtacggacc atctttaaag agtggcgtat ggaaagtcag
    48901 aatatccgcg cgctggacta actcatccag cgagcggaaa tcaccctcat ccccacggtc
    48961 ggcgcgaggc ggatcgcaaa gtaaggtttt aatccctaac gcttccagtc gcgcctgcaa
    49021 tcgacgtcca acgttaccaa cgcccacgat ccccaccgta cggtcgtgca gtgaaaatcc
    49081 atcgcgttcg gcaagcatca gcagggagga gaaaacatat tccaccaccg caatcgcatt
    49141 acagccaggt gcagcggaaa aaccaattcc cgcctgcttc aaccatgctt catcgacatg
    49201 gtcggtcccc gcagtggcag ttccaacaaa tttaatgggt tttcctgcca gcaaagattc
    49261 attcactttc gtgaccgaac gcaccatcag cgcatccgcg tctgccagtt gagcgacggg
    49321 gattggacgc ccgggaaccg cgatcacctc acccaaacgg ctaaataagt cgcgggcata
    49381 aggcatattt tcatcaacaa ggattttcac gtttgtgtta cctgtatgag acgagagtta
    49441 accggacaag tatgccataa tctcgcggcc aggcatactt gcgaagattt caggtataag
    49501 gatacgtaat gatacaacct atttccggcc ctcctcctgg gcagccacca ggtcagggag
    49561 ataacctgcc gtctggcgcg ggcaatcagc ctttatccag tcagcaacgt acttcgctgg
    49621 aaagcttaat gacgaaagtg acctcactga cgcaacaaca aagagcagaa ctgtgggcgg
    49681 gtatcaggca cgatattggt ctgtcgggag attcaccgct gctttcgcgt cacttccctg
    49741 ccgctgagca taatctggcg caacgtctgc tggccgcgca aaaaagccat tctgcccgcc
    49801 agcttttagc gcaattaggg gagtatttac gtctggggaa tcatcgtcag gcggtcacgg
    49861 attatatccg tcataacttt ggtcagacgc cgctgaatca gctctcaccg gagcaattaa
    49921 aaaccattct caccctgttg caggaaggga agatggttat tccgcaacca cagcagcgcg
    49981 aggcgaccga ccgtccttta ttaccggcgg agcacaatgc gctaaaacag ctggtgacca
    50041 aacttgcggc ggcaacgggg gaacccagca aacagatctg gcaatcgatg ctggaacttt
    50101 ccggggtgaa agatggcgag ttaattccag cgaaactgtt taaccatctg gtgacctggc
    50161 tacaggcgcg tcagacgcta agccagcaaa atacgccgac gctggaatca ctacagatgg
    50221 cgctaaaaca acctttagat gccagtgaac tggcggcgtt atcggcatat atccagcaaa
    50281 aatatggcct ttctgcgcaa tcatcgcttt cttctgccca ggccgaggat attcttaatc
    50341 agctttatca acggcgggtt aaagggattg atccgcgtga tatgcaaccg ctgcttaatc
    50401 cttttccacc gatgatggac acgttgcaaa atatggcaac gcgtcccgcg ctgtggatac
    50461 tgttagtcgc gattatcctg atgctggtct ggctggttcg ttaaccccga cgaaatgaca
    50521 gtatcgtgac aataataccc agcaccgcag agatcgcccc ggcaagaaat accgaagagt
    50581 aaccaaacgt ggtcgccagc attcccgcca gcggcccgga gacgccgagg gcgatatcct
    50641 gaaacgcggc gtaaccgccc agtgcggtgc cgcgaacttg tgaggggacg cgtttaacca
    50701 cctccacgcc cagcgcagga aagataagcg aacatccggc tccggttaac gccgcgcccg
    50761 ctaatgcgac ccatgcacct ggggcttgcc agagcagcaa caagcccacc gtttctacaa
    50821 gcagagagac aatcgccact ttcacgccgc caaaacggtc cggcatccag ccaaacatga
    50881 cgcgcatcac gacaaatgcg ccgccaaacg cggtaagagt aaagcccgcc atcgcccatc
    50941 ctttgctggc aaagtagagc gaaacgaaag tcccgataac cgcaaaacca acgccttgta
    51001 gtgccagccc taaccctggt ttccagataa gcccgacaac gctccacagc gatggacgtt
    51061 ctcccgccag ggccggtact ttgcgcactg tgccgttaca ggcccacgcc agtaagggta
    51121 ataccattgt ggtgatcgcc agtgcggcaa aaccgtaatg gctatgaatc aacaggccaa
    51181 gcggcgcacc aacagcgagg gcaccgtaaa tcgccattcc gttccatgac atcactttgc
    51241 cagagtgttt tggccctacg atgcctaacc cccaggtcag agcgcctgtc agtaactggc
    51301 tttcaccaaa cccaagaatc aaacgcccga tgaccaacag ggcaaatttg aacggtgcgg
    51361 agacaggcaa aatcgccgcc agcagcaacg cgccgccagc cagaccacaa gctaacatcc
    51421 cctgaagcgc cgaacgtttt gcgccatatt gatcggccag tcgcccggcg taaccacgcg
    51481 tcagcaccgt agccagaaac tgaatcccga cggcaatgcc gaccatggta ttgccatagc
    51541 ccagatcatg atgaacaaac agcgggataa ccggcaacgg caaccctacg gtcatgtagg
    51601 tgagaaaaac cgcaaaagag atgcggaaga gcgaaaaact ggcagaagat cgtgtttcgg
    51661 tttggcttac agcagtcatg cattactcca gaatgcagcg caaggcgagg agtatccccg
    51721 tctcatctct ctggtttcag ggttacggtg cgttggcagg atttaacgcg tacgtctttt
    51781 cagaaggaaa tcgacaaagc gggaagtttg cctagaactg gtggcgattg tcaatgatgt
    51841 gaaaaaggga accatcaggt tcccttttgc gttagtgccg gaggccgcat tggcgcgtaa
    51901 cgtcggatgc gacgctggcg cgtcttatcc gacctactcc ggcctactgc gaattaatct
    51961 tttcagcttg cgcattacca gcgtggcgtt ggtgccgccg aagccgaagc tgttagacat
    52021 aacagtggtc agttcgcgat cggtcgtttc ggtcacgatg ttcagacccg cagcctgctc
    52081 gtccagctct tcaatgttga tgctcggggc gataaagccg tgttccagca tcagcagaga
    52141 gtagatagct tcctgcacac ccgcagcgcc cagtgagtga ccggtcatgg ctttggttgc
    52201 agaaatcgcc gggctcttat cgccgaacac ttcacggata gctgccagct ctttcacgtc
    52261 gccaaccgga gtcgaagtac cgtgagagtt caggtaatcg attggggtat caacgccatg
    52321 catcgccatc tgcatacaac gtactgcgcc ttcgccagac ggagcaacca tgtctgcacc
    52381 atcagaggtt gcgccgtagc caacgatttc agcatagatg taagcaccac gcgccagcgc
    52441 gtgttccagc tcttcaacca ctaccatacc gccgccgcca gcgataacga aaccgtcacg
    52501 gtgagcgtcg taagtacggg aggctttttc cggggtgtcg ttgtatttag tagatagcgc
    52561 acccatcgcg tcgaattcgc aggccatttc ccagcacagc tcttcgccgc cgccagcaaa
    52621 cacgatgtcc tgtttgccca gttggatctg ctctactgcg ttaccgatac agtgtgcgga
    52681 agtcgcacac gcggagctga tggagtagtt aacgccatga attttaaacg gcgtggcgag
    52741 acaggcagaa acgccggatg ccatcgcttt agtgaccaca tacgggccaa ccgctttcag
    52801 gccgcgcggg ccgcgcattg cgtcagcgcc gaacacctgg aaacgcgggg aaccgccgcc
    52861 ggaacctgca atcaggccaa cgcgcgggtt attctggtaa gcttccggag agaggcccgc
    52921 atcagcgatt gcctgctcca tagaaaggaa tgcataaatg gatgcgtcgc tcataaagcg
    52981 cacaactttg cggtcaatga ggccagtggt atccagtttt atgttgcccc agacgtggct
    53041 acgcatgccg gaatccttca gctcctgaga gaaagtgatc cctgaacgtc cttcacgcag
    53101 agatgccagg acttcctgct ggttattacc gatgctggaa acaatgccca ggccagtaat
    53161 cactgcacgt ttcattcaat acctctgtaa gtcgcacata gagtaagttt cgaatgcaca
    53221 atagcgtaca cttgtacgcc gaacaagtcc gatcagccat ttaatagaga tatttgcgca
    53281 gccttacaca catcgctaag atcgagccac cgcctgtaag acgagtaact tacgtgaaac
    53341 actactccat acaacctgcc aacctcgaat ttaatgctga gggtacacct gtttcccgag
    53401 attttgacga tgtctatttt tccaacgata acgggctgga agagacgcgt tatgtttttc
    53461 tgggaggtaa ccaattagag gtacgctttc ctgagcatcc acatcctctg tttgtgatag
    53521 cagagagcgg cttcggcacc ggattaaact tcctgacgct atggaaggca tttgatcagt
    53581 ttcgcgaagc gcatccgcaa gcgcaattac aacgcttaca tttcattagt tttgagaaat
    53641 ttcccctcac ccgtgcggat ttagccttag cgcatcaaca ctggccggaa ctggctccgt
    53701 gggcagaaca acttcaggcg cagtggccaa tgcccttgcc cggttgccat cgtttattgc
    53761 tcgatgaagg ccgcgtgacg ctggatttat ggtttggcga tattaacgaa ctgaccagcc
    53821 aactggacga ttcgctaaat caaaaagtag atgcctggtt tctggacggc tttgcgccag
    53881 ctaaaaaccc ggatatgtgg acgcaaaatc tgtttaacgc catggcaagg ttggcgcgtc
    53941 caggcggcac gctggcgaca tttacgtctg ccggttttgt ccgccgcggt ttgcaggaag
    54001 ccggattcac gatgcaaaaa cgtaagggct ttgggcgcaa acgggaaatg ctttgtgggg
    54061 tgatggaaca gacattacca ctcccctgct ccacgccgtg gtttaatcgt acgggcagca
    54121 gcaaacggga agtagcgatt atcggcggtg gtattgccag cgcgttgttg tcgctggcgt
    54181 tattacggcg cggctggcag gtagcgcttt attgcgcgga tgaagccccc gcgctgggtg
    54241 cttccggcaa tcgccagggg gcgctgtatc cgttattaag caaacacgat gaggcgctaa
    54301 accgcttttt ctctaatgcg tttacttttg ctcgtcggtt ttacgaccaa ttacccgtta
    54361 aatttgatca tgactggtgc ggcgtcacgc agttaggctg ggatgagaaa agccagcata
    54421 aaatcgcaca gatgttgtca atggatttac ccgcagaact ggctgtagcc gttgaggcaa
    54481 atgcggttga acaaattacg ggcgttgcga caaattgcag cggcattact tatccgcaag
    54541 gtggttggct gtgcccagca gaactgaccc gtaatgtgct ggaactggcg caacagcagg
    54601 gtttgcagat ttattatcaa tatcagttac agaatttatc ccgtaaggat gactgttggt
    54661 tgttgaattt tgcaggagat cagcaagcaa cacacagcgt agtggtactg gcgaacgggc
    54721 atcaaatcag ccgtttcagc caaacgtcga ctctcccggt gtattcggtt gccgggcagg
    54781 tcagccatat tccgacaacg ccggaactgg cagagctgaa gcaggtgctg tgctatgacg
    54841 gttatctcac gccacaaaat ccggcgaatc aacatcattg tattggtgcc agttatcatc
    54901 gcggcagcga agatacggcg tacagtgagg acgatcagca gcagaatcgc cagcggttga
    54961 ttgattgttt cccgcaggca cagtgggcaa aagaggttga tgtcagtgat aaagaggcgc
    55021 gctgcggtgt gcgttgtgcc acccgcgatc atctgccaat ggtaggcaat gttcccgatt
    55081 atgaggcaac actcgtggaa tatgcgtcgt tggcggagca aaaagatgag gcggtaagcg
    55141 cgccggtttt tgacgatctc tttatgtttg cggctttagg ttctcgcggt ttgtgttctg
    55201 ccccgctgtg tgccgagatt ctggcggcgc agatgagcga cgaaccgatt ccgatggatg
    55261 ccagtacgct ggcggcgtta aacccgaatc ggttatgggt gcggaaattg ttgaagggta
    55321 aagcggttaa ggcggggtaa tctgctctcg cagtgtttgc cggatgcggc gtgaacgcct
    55381 tatccaacct acgtgtgacg tgtaggcctg ataagacgcg ataagcgtcg catcaggcat
    55441 ggtgctccag acgccgcaac ttacagctgt gacgcctgct gaaacaggtt ttcccacata
    55501 tcggtcacca gcgcctggtc acgcggcgac aattcaccgg caccaatggc tttttccaga
    55561 ctctggctaa cggtcgtatg caccgcctga gcggagtggt catcaccact ttccagttct
    55621 gcgatggcta atgtcaggtg gccacgcaaa tacccactgg caaacaactc atcatcactg
    55681 gcatggtcaa ccatgccgtc gattaatgcc agaatgcgtg attcaaactc cgcgatcatc
    55741 ttctttcctc attgtaaaac gtggcgctga cttcagttga gcgcttccgg ccacgggaac
    55801 tgttccgccg taagttccgg cgtgtgataa taattttgta gtgctttaat gaagcgtgcc
    55861 gggcgttccg gaatgccatt tgtcagataa tccatcacct gcgcatgtac ccgccgttga
    55921 aacaccacac gatccggttc gaaatcccct tccagattgt cgcaactgac gttaaacgga
    55981 tatccagccg ccacgcagaa taaccagtcg aacgcctgcg gcttcacttc aacatcttca
    56041 aactggcttt gcgtctgggc atcgcgtccg tccgggcaat accagtagcc gaaatcaacc
    56101 agctcacggc gcgcttttcc ggcaatacac cagtgcgaaa tctcatgaat tgcgctggca
    56161 taaaagccat gagcaaagac gattcggtta tacggtactt ccgcatcagc aggaagatag
    56221 atcggttcgt cgtcgccttt aatcagacgg gtattaaatt catcggcaaa gcagctatta
    56281 aaaatttcaa tcaactgctc gtagtggtgt gtactgttca ttagttcatc cccaaccagt
    56341 ggaggatctc ctgtccgtgg ctatcgtaaa gtaatttggc actcatcacc gccgagacga
    56401 taacaatcat cgggcggatc agcttttgtc ctttgctcaa caccaaccgt gaacccatgc
    56461 gcgcgccgag gaactgcccc accagcatga caaagcccgt cgcccaaatc actttgccgc
    56521 cgagaataaa cagcagcaga ccgccaatgt ttgacgttgc gttgagtaat ttggcgtgag
    56581 ccgtggcttt ggcgaggttg aatccgcaca gcataacgaa ggccagcgcg taaaacgatc
    56641 cggcagccgg gccaaagaat ccgtcataaa aaccgacgca gccaccggca attaacgcga
    56701 agggtaaacc gtacatccgg cgctggcgat cttcttcacc cagctttggc atcagcaaga
    56761 aatagaggcc gatacaaatc accagaatgg gcaaaatctg ccgtaagaca tcagcctgaa
    56821 cgtactgtac caacagtgcg ccgctcattg agccgacaaa ggtcatggca atattgagtt
    56881 tctgatcgct taaactaacc actttgcggc gaataaagta gatagtggcg gaaatagagc
    56941 cgccgcaggc ttgcagttta ttggttgcca gcgcgttagc gggagacatt cccgcagcca
    57001 tcaatgccgg aatggtgagt aacccaccgc caccggcaat cgagtcgata aatcccgcca
    57061 gcatggcgac aaaaaagaga actcccagca acagcggaga aaccataaac aggctattaa
    57121 acgtttccat tagatcacgt gctcatccag tagcgcctgg caggaaggcg gcaacggagg
    57181 cggtgtcttc ttctcaggct ttgttgttcc cggttttgga ggttcaaacc agctttgcag
    57241 ttctgccccg caaccatcgc ctgatggcgg taaaggttga tcttcacact ccagactatc
    57301 ggcagggcaa cgtaatcgta catgcatatg cgcgcgatgc tggaaccagg gtcgcacttt
    57361 gcgcaaccag tcgcgatcgg tgcccgcatc aaggcaaagt tgttgtttaa tcgccggatt
    57421 aacaaaaatg cgcgtgacgt ctttgtcctg ggcggcgagt ttgatcaagc tgaaaatttc
    57481 tggcttccac agcgtggaga caacgtgttt accgtcgcgg gaaactaagt ccagtgcttg
    57541 cgggcgcaag agctgcgcgg aggtccagcg agttttcggc agttgcagaa agatatcgac
    57601 atccagtccg gtctggtggc tggcatgacc gccgttgaaa cgcccaccag cgggcatccc
    57661 catatcgcca atcagcaccg tacccatgcc cagattgctc acctggctac tcagacgctg
    57721 gataaacatc accagatccg ggtgaccgaa atagcgacgc tgatcggtac gcatgacctg
    57781 ataatgttcg gactgtatcg gcaacgtgtc agcgccgaca atacagccat tagaaaaact
    57841 gcctatcgat tgtgcgctac ccggcacagg ttgggttatt ttttgccacg gcgtcgctgc
    57901 caggctagcg ctactggcaa gcagagccag cagcgcaatc gcggttttgt tcatttttta
    57961 ccagcgtgga atatcagtct tcacatcggc attttgcgcc cgctgccgta acaggtgatc
    58021 cattaaaacg atcgccagca tcgcttcagc gatcggcact gcacggatcc cgacacaggg
    58081 atcgtgacgg cctttggtga tcatctcaac ttcttcgcca aagcggttaa tagtgcgccc
    58141 cggcacggta atgctggagg tcggtttcag cgccatatga gcaatgattt gctgcccgct
    58201 gctgataccg ccgaaaatgc cgcccgcatg gttgctctgg aaaccgtctt tggtgatttc
    58261 gtcgcggttc tggctgccgc gcagcgccac tacgtcaaaa ccgtcgccaa tttccacgcc
    58321 tttcaccgcg ttgatgctca tcagcgcatg ggcgatgtcg gcatccaggc ggtcaaagac
    58381 cggctcgcca agtccggcgg ggacaccact ggcaacaacg gtgactttcg cgccgatgga
    58441 gtcgccctct tttttcagcg cgcgcatcag ttcatctaac gcgtcgattt tgtcggggtc
    58501 cgggcagaag aacggatttt gttcaaccag cgaccagtct ttgatttcca gcggaatgtc
    58561 gcccatctgg gtcaggcagc cgcggatttc aataccaaat ttctcggcga gatatttttt
    58621 ggcaatcgcc cctgccgcca cgcgcatggc ggtttcgcgg gcggaagaac gtccaccgcc
    58681 gcgataatcg cgcagaccgt atttttgttc gtaggtgtaa tcagcatggc ctggacggaa
    58741 gacgtcttta attgcgctgt aatcctgaga acgctggtcg gtattttcga tcaacaagcc
    58801 aatgctggtg ccagtagtaa cgccttcaaa aacaccggag agtattttga cctgatccgg
    58861 ctcgcggcgc tgggtggtat agcgcgatgt cccagggcga cgacgatcaa ggtcatgttg
    58921 caggtccgct tccgtcagcg gaatgcctgg cggaacacca tcgacgatgc agccgagcgc
    58981 cagcccgtgc gattcgccga aggtggttac gcgaaagagt tgtccaattg tgtttccagc
    59041 catcacggct ccgttattgt tgtgtttgcg tgtttactta atctttataa atcgcgaaat
    59101 gttctcgtgc ggcaataagc tgctctttag tgagcataaa cacaccatcg ccgccgttat
    59161 caaactccag ccaggtgaac ggaacatcgg gatattgttc cataagatgt accatgctgt
    59221 tgcccacttc acaaatcaac acgccatcat cagcaaggta atctgccgcg ttaccgagaa
    59281 tgcgacgcgt cagtttcagg ccgtcggtgc cagatgccag gcccagttcc ggctcgtgac
    59341 ggtattcgtt tggcaggtcg gacatatctt ccgcgtcgac atacggcggg ttagtgacaa
    59401 tcagatcgta ttgcactttc ggcaaatcac ggaacagatc ggaacgaatc ggaatgacgt
    59461 tgtggatcag accgtgttct tcgatgttct gttcggcaac cgccagcgcg tcgggagaga
    59521 tatctaccgc gtcgacttcc gcttccggga aggcataagc acaggcaatg gcgatgcagc
    59581 cgctaccggt acacatatct aaaatatgct gcggttgctt gctgatgagt ccggcaaatt
    59641 tattgttgat cagttcacca atcggcgagc gcggcaccag cacgcgttca tcgacgtaaa
    59701 attcatgacc gcagaaccag gctttgttgg tcaggtaagc caccggaatg cgctcgttga
    59761 cgcggcggat cacgcgttca acaatacggt gtttttcgct ggaggtcaga cgcgcggtgc
    59821 gcatatcttc cgggatatcc agcggcaggt agagcgatgg taacaccagc tgtacggctt
    59881 catcccacgg gttatcggtg ccgtgaccgt accagatatt tgccgcgctg aagcggctta
    59941 ccgaccagcg caacatgtcc tgaatggttt gcagctcatt tactgcttca tcaacgaaaa
    60001 ttttatccac gtattcctcc agggcatgat cgcaataatt tcggcggcta gtttgccatg
    60061 aagatgacga taaatcagca ttcacgcgcg gtgagtgagg aaaaatacgt ttaaaacgat
    60121 cgattgtgct acgagtcggg taaactgtag gaaaattaga aatagagaca gataaatgaa
    60181 aaagaaaaca acacttagcg aggaggacca ggctctgttt cgccagttga tggcggggac
    60241 tcgcaagatt aagcaggaca cgattgtcca ccgaccgcag cgtaaaaaaa tcagcgaagt
    60301 gccggtgaaa cgcttgatcc aggagcaggc tgatgccagc cattatttct ccgatgagtt
    60361 tcagccgtta ttaaataccg aaggtccggt gaaatatgtt cgcccggatg tcagccattt
    60421 tgaggcgaag aaactgcgcc gcggcgatta ttcaccggaa ttgtttttgg atttacacgg
    60481 tctgacgcag ctgcaggcca agcaggaact gggcgcgttg attgccgcct gtcgccgcga
    60541 acatgtgttt tgtgcctgcg tgatgcatgg gcacgggaag catattttga agcaacaaac
    60601 accgctgtgg ctggcacaac atccgcatgt gatggcattt caccaggcac cgaaagagta
    60661 cggcggtgat gcggctttgt tggtgttgat tgaagtggaa gaatggttgc cgccggagtt
    60721 gccctgaatg ctggcagcaa atgccggagc agcgtaaacg ccttatccgg cctacaaata
    60781 tgtgataatt caataaattg caattcactt cgtaggcctg ataagacgca tcagagcaat
    60841 actacgaact tcagttcatt caatacatac tgaaaacgac acgtcgaatc aatcggttgc
    60901 ccactactgg tattcacatt caccacaata tcgtgtagta ctttatccgt taccggatga
    60961 cgagttggcg taaccgttac cgttgattta tcacaaccgt aagaggtaaa ggttaacaat
    61021 tctgataccg cgcggcagta tgctcctctg ccatcattat cagaaaaatt actgccatca
    61081 cagagtctgc cgccttccat caggtaggtc gaaagcgtgg tggtcactgt tgaccccgtg
    61141 gactgaattc gtaacgcctt attatggctg acataatttt cactccagga gatatcgtat
    61201 gaagcgaccg gtatcgttgg gcatattgtg ttatgcactg taggggccgt atagcttcca
    61261 aatccgggta aatctgtata ggaataaaag gatgcgatcc aagggcaacc ataatcatca
    61321 gatgcaacgg taattttaaa cgtcatatat gcacaacgaa aaattttcgt atctatgcca
    61381 gggcaatttg agttattaat tgtatcaaag tcagtcagta ccaaagttgg agctgaaccc
    61441 gacccagacc aggaaataac ccgcaacgta tcgcctttat acgcaaaaga atcatatttc
    61501 ccatatgcaa cctcctttgg cgttatcacc gtaacattga aaacactgct cgctacagca
    61561 ccggttacct tgtccatggc ataataaact ctcacactgt caaaactcgc actcataagt
    61621 ttatctccag cataactctt agatgcgccg acaacactca gaacgagcag cattaggctc
    61681 gcgaaaatta tggtccattt tttcatttca gaaatactca aaggttgccg tcactgtggc
    61741 actaaagtct ccggacgtta catctcgccc gctctttgcc tgtaaccagg tattaaaatt
    61801 taggctgtta tttccctgat tcagcgggag cgtgacgcct gttgtattgt tgattggcac
    61861 cgatgtgcct gccgcagttt caataccaat cccagcgccg gatgctgatg acgaggagtc
    61921 aaaggccaga aagccgggta gagcactatc ttctgttcct gtcaacgtta ctttgacatt
    61981 aaacaatgta gaactatgac aatctttcaa ttgaaacacc aatggcactc gctctgactg
    62041 cccaaggttc attaaatcac tggcggcaat ggcaggaaaa tgtacttctg ccaactggct
    62101 accattaatc actggcgtac acgatttact gattaaattg ccggtaaagt gcaaattatc
    62161 tcctgctatt gtggttccac tgcccagcaa catcaggcta ctaataaaaa acaacgtacg
    62221 ctttttcttc attggtaatc tacctgtaac gtggcccagg cctcaaagtc cgcctctggc
    62281 agaatgacac cactcttttt tactggcaca gccgttaaca ccggtaaatc ggtttcattc
    62341 accacaagtg gcgtattgat cgtaaaactc tgccctgcct gtttgagctg aatgcctaaa
    62401 ccagtgatat tgctgcttac tgcagcatta tcaaaatcgc ttggtgtgcc actccagctc
    62461 aaggtcattt ccattaatgg gtcgcgaacc gccgtatcgc aggtaatgga atacggcacg
    62521 gtttgctcat aattgctgcc attaatgtca tcaataagca catcctgaaa agttatttca
    62581 atggtttgag cgttgctaat cgtgcagtta ggcggctgca ctaaataacc gtgaaaagag
    62641 acgtttgaga gtgccatcga gcaaaagccc cacagcaaaa taagcgagat ccgtttcatt
    62701 ggtaatccac cacaattgtg gcactggcgt taaattcagc tgccgctaac gttgtaccac
    62761 tctgtttcac cggcacagct tccagaacgg gaactcccga ggaaaaatta aatggcaacc
    62821 atgagccaga ggttaagtcg agtattgtgt gatcactact tttctgaacc cgaataccaa
    62881 acccaggaat acccgtgctg ataactgttt caccgttaat cacgaccgtt gtcgcctgca
    62941 tctgtagacg caggtcatcc attgccagat tgttacaaac aagcgagtag tcaatgggac
    63001 gtttataatc aaccctattg atcttattaa tgaatacatt accaaactca actgccgcac
    63061 catttaccgt acaaggagga ggggcatcga caagcacccg taaattcata cttactgact
    63121 tactcgctgc aactgcaaag ccgctaccaa cacaacatat cagtaaccca gttaccgccc
    63181 agacagaaca agatcctttc atactggctc tcccggcctc agcctttgcg gctctgctct
    63241 tcatcgacct agcaactgtt accgttgcaa gtgaaaaaga gcggcatccg agcgccatag
    63301 tcgttgacat aggtcagtac cggcacgcta cccatcttca cattcagcgg tgcagaggtt
    63361 ttcggcgaca tcactattag cgagaaaccg gctgccggat tgccattttt ctgtgtgctg
    63421 gcattactga aaatgacgta atacgacgtc gggttattga cggtaaattc ctgaccgttg
    63481 cgggttaaag taatcttata ttgctagggg tttttcatgc tgacgttctc cagcgccttc
    63541 ggtcgccaga acaacttaat acgcgtttgt agtgctattt gcagcgtgtt ggctttgttt
    63601 gattttggcg ggatctcgcg cacgttgaag tagaacaggc tttcacgatc ggcaggcaat
    63661 ttgttgatat ccggcattcc ctgcactttt acctgcccgt tcatcattga atcgatgcgt
    63721 tgcaccggtg gcagaacagt taacggtgaa ctgatcttgt tacccttttc atcttcgatc
    63781 cagctttggg ccagataagg cagtttcgga tcgttattgc gcaacgtcac gctgattgat
    63841 ttgtcgcttt cattaaatat caaacgggta cgatctggcg ttacagaagc ctgggcagac
    63901 aaactggcag cgctaagcag taacgctagc gtcgtagcac ccaatttggc agagcaaagt
    63961 aaatcagaca tggatcattc cttaatcacg ttattggtta gctgaaacag aaacaggggg
    64021 attggtgggt gtcacctgtt gcgtctgctc ctgaatgaca ggctttatgt catcaggaac
    64081 aaccggcgcg acgttgcctt tatgctggca tggcagtaac aagccgttaa acagatcggc
    64141 aggcagcggg tccggcaggt tgatatcgca atgcgcaaca ccactccaga acacactcat
    64201 gtgttcgcca ggtttcaccc cagccagata aacattgccg tcatcatcga caaggcccac
    64261 tgtctgctcg ttatcatttt ttacttctgc gccaaacgga ggatggctgc cgtcttgtaa
    64321 acgcagcacc gccatcgctt tttgaccact gatgacggca aatttgcggt agccaatcgc
    64381 cccttcagtt agcgtggctt gtaccaccga ctgggttgct tcggcgtttt ccggcagttt
    64441 gttgaggtcg atatacgcct gattgcggta atagttattg acgtcagaaa cgacggcttt
    64501 accaaacata ttggtataaa cagcagcacc gttaccttct accggaacat cggcaacgcc
    64561 atcggcatca atcaacaagc gtgtcccgcc catattctgg gtacggtgaa gtgcgccacc
    64621 gtgggcagta agcgttgcgc cgccctgtaa cgacaagccc gcagaggtgt attgcccttc
    64681 atggtagttc gcactgaggt caacctgcgc cagcgaacca tcatggctgt aatagccgtc
    64741 aacgctggtg tgtttgtcac tggtgccgac gttcaactga tagtgagtcg cgtcatcgac
    64801 acggctgaaa taaccgacct gactgctgtc cgtcccactg ccatagtacc gttatagcta
    64861 acggtactgt tgtcgcccca cggcatactg agcgaaatgt acatgccttt gtcggcctgg
    64921 ttgtcatact cgtaacggta gccagtcatc gagatgctga cattacgaat actgcccata
    64981 ttgaaatagt gcgagagcat gatgttgtag tttgtctgct cctcgcgatc ccagtaggta
    65041 tggcgggtat agttgagata aaccgaaaca cccgcatcgc ggaagttctg gttataagtg
    65101 gcggtgtaca tctctttgtc gttgcccgtg cgtaccattc cgctgtcact ggcatccaga
    65161 tactcgctca tggtcataaa gttctcttcc gagaagcgat atccagcaaa agtaacgcgg
    65221 ctgttgagct ggtcaaagtc tttggaataa ctcacacgga aggagttacc gtccagcgaa
    65281 cctttgccat aagcggtgtc tttatccagt ttggtgtgcg agtgagtaac atcaaacgcg
    65341 accgcgccga atgtagacaa atcgcgaccg acaccaagcg ccgcagactg atagttttca
    65401 tctcccagtg cgccgccata taacgaccag ccgttagcga tcccccagga agcttcagca
    65461 ccagaaaaaa attcaccctc gacatggtgt ccccactctt gcggacggcc catcatgatc
    65521 ttatagcgaa cctgacctgg acgagtgagg tatggcatcg aggcggtgct gatgtcatat
    65581 tcctgcacct ggccgttctg ttcttcaatg cgaatatgca acgtaccgga gacagaatca
    65641 ccaagatcct gaatacgaaa cggtccagcc ggaacctgcg tttcgtaaat cacacgcccc
    65701 atctggctga cggtcacttt tgctgtggtg tgtgccacgc cggaaatgtc tggcgcgtag
    65761 ccgcgcagat tgggaggcaa catttgatcg tcagtactga cactgccacc aacatagtta
    65821 aaaccatcaa aaatatcgga tctgaggtaa tcctcgccca gcgccagttt ggcttttaat
    65881 gatggtaacg cccgcctggc atagtagcga ctccactccc attttttttg agtttcatcg
    65941 ccgctgaatt catcgtcatc attactgcga gtatgttgat agttagtctg ccagtcagca
    66001 cgcatacgcc acggccctag gttaacccca accgtcccgt tgccgctgat ctcgttacta
    66061 tcatcaccgc cattttcttc gtgccgggtt tgtgcgttga tgctgtagtc cgcgacgatc
    66121 ccggagatgc cgtcatccca acgtgaaggc ggatcccagt cgggataagt atattcgagg
    66181 taagcctgtg gcagtgaaat gactaatgcg gactggctta aatcagcctt aatttccatg
    66241 ccttccagtt gaccggattt caggcattta gcatcgtggc tccattgcag atttttcgcc
    66301 acgtcttctt ttaaaccaaa ctgcgctacc agttccggtg tcagacaagc atagcttttg
    66361 ctcgcgtcat cttcaccagc ataccagtaa atatcgtact cttccgccaa tggctgttta
    66421 tttagttgaa cctgtaaatt gtattttccg ggctcaacat atccctagct ggaaaaacgc
    66481 ttcagatcaa tttttgtgtc gccttttaat tccagaaaac gggaatcaaa ctgaatgtcg
    66541 tcttcagccc agacactgct ataactccct gacattgcca gcgcaatgca ccacggaaga
    66601 atccgtaatc gaaaaagaga atggtcaggc atacacgttt ctatccgttt ttattcttgt
    66661 tcggtcaaaa tacatttaaa tcccccatag cagtgtcgct atgggggtat gccttacagg
    66721 taagtaatct ggaaggtggt atttgcttca aaattaccca gatctggcgc atcagctgcg
    66781 ccgaccagcc aggcgttaaa gttcagtgtc tgcttcgctt tgcctttgtt ggtagaagtg
    66841 tctttcacta ttttctggtc aatacccatg ccgcttttgt aagaggtacc cagagagtca
    66901 ccgatcgcca ggctaacatt gttaaatgcc gcaccggtat cggtattgaa aatggtgtaa
    66961 taattgccgc tatttgcaga agaaacggta ccagtaaagg tagtggtcat tgtttcctga
    67021 gtatcaaata cgcaatcttg cagacgaatc tggaaacttt tcggcgtggt ggtgccgtta
    67081 tttttcagtt tttcggcacc gatatcaccc atatcaactt ccagtttgtg atcgtctgga
    67141 acgatagaac acactgagtc ttcaatggtg ccgtaaaaac gtgcagtacc gttgttaccc
    67201 gcagcgattg ttgccgtaga ggtgaacacg cccatcacca ttgccgcagc aatagctgtt
    67261 ttaacaaact tactcattat ttgtccttaa actataaata attaaaaata taaacattgc
    67321 aatacattga ttcagtcaat agccaatgtt tatacccagc cattgaatac ggcatgcgac
    67381 ccaaaaagaa caggtcgtag aaaattgtat tcattggtgt ggggccgcat agtgatgttt
    67441 ccacacccct gatccaattt aattttacta aagagtaaat gtagatttaa ttaatatatt
    67501 gacagaagaa aggttatctt aaggacatcc ttaaaaaata cggaataagt atttagaaaa
    67561 aatagaaagt atatcaataa tgaatatcaa cagcttaaaa aacaaacaat gaacatataa
    67621 tgcgattggc atttatccac ttttgacaat ataatatata aacactatag ccagaattta
    67681 atcaagtttc aattacatcc tgcctgatta ttcaactcta ttttctctat tatctgtatt
    67741 aataaatgac aatgagtgtc aaacagatat tattcatttc aacatgtaac tttgggaaaa
    67801 atcaagatac gcgcagaaga ataatttccg aatgaatgta gaaagtgttt tatttataga
    67861 taaggagagt cttcgatagt agtgaggttg acagatatcc gctgcctaat gagcaattat
    67921 gcccaatgaa tctacctcat tgggcataat ttggaacaca tcagatagct tttgccatct
    67981 tcagattaca cgggctcatc tgccagttga atgtaccgtt tccgctttca tcaagcgtta
    68041 cgctggcaat ggccgaggtg gtaaacatag gcggcgtttc gcccggacat aactcggcaa
    68101 ccagataccc gactaacggt aagtgagaga tcaccagtac cgaagcaaca ccttcattgg
    68161 tcaacgcctg caaataggca ctgaccagac caacatcgcc gcagggcgtt aactccggca
    68221 gaacttctgc actggaaggc aggttcagac aatcccctac ttcttccagt gtttgctcgg
    68281 ctcgcaggaa cgggctcacc agaacgcgtt cgatttccac tttttgacct ttcagccagt
    68341 tcgccatcag gcgagattcg tcacaaccat tagtggtcag aggacgaacc gaatcactgg
    68401 cggaatcgag ggctgcgtcg ccgtgacgca tgataaaaac ttgcatattg caccgctttt
    68461 gttaaccaga ttcgcccgtt ttctttcatc tgaaaccaga gaaagaaaac ggtggccggc
    68521 attgtgcctt atccattcac cgaatgaaac gctgtttttt acctcaatgg cgtaagtata
    68581 gtcaatatgc gtttacattt tgaccaacac tccgccattc agcgcggatt catatagctt
    68641 tgaccttctt attgcaggtc agttgcagtt gttttccaaa aactttcccc acgcgcgccc
    68701 atctcgacca aacgctcgca aggggtaaaa cgggaaccat actgcgtggc cagtcgttgc
    68761 attattgcaa ccacttcgcc cgcgccgaga gaatcgatat agcggaacgg tccaccgaga
    68821 aatggcggaa aaccaatgcc aaataccgca ccaatatccc cgtcacgcac gctacggata
    68881 acctgctcat caacacaacg tactgcttca ttcagcatca acatcacaca ccgttcagca
    68941 acctgcggtg cggagagtca cccctgccct tgtgcgccaa tgagcgggta aatggcagga
    69001 tcgacctgtt ttttgctttt acgccctttc tgaccataaa gatagaaacc ccgaccattt
    69061 tttctgcctt tgcgatcgtc gttcaaaatt gaagaaacaa catttgcagg cgcgctaaaa
    69121 cgttctccat aagcggcttc cagtacagga ataattttag tcccggtgtc gattcctacc
    69181 tcatccaaaa gttggattgg gcctaccgga aaaccaaatt tcactagcgc ggcatcaatg
    69241 tgctctaccc gttcaccttc ggtcagcatg cggatagctt cattaatgta aggcgctaag
    69301 atgcgattga cgtaaaaccc ggctttgtca cgcacgacaa tgggcgtttt gccctgtttt
    69361 ttcgccagtt ttaccgtagt ggcgatggtt tgcgccgatg tacccgcatg aggaataatc
    69421 tctaccagcg gcattttttc caccggactg aagaaatgca ggccaataac ttgctcaggt
    69481 cgcgcggcgt gagcggcgat atcaccaatc ggtaaagatg acgtgttcga agcaaaaatg
    69541 gtatgagcgg cgcaattttg ctccacttcc gccaccatct gttgttttaa ttcgagattt
    69601 tcaaacaccg cttcaataat cagatcgcga tgggcaaagc cgcgatagtc cgtcgttccg
    69661 gagattaatg ccagctgttt gtcacgttcg ctggctttga gatgacgacg gcgaactttg
    69721 ccctccagct gatcccaact gtacttcagc gcatgattta tgccctgcgg gttgatatct
    69781 ttaattctga ccggaagccc cgctttacaa gcagtgacat aagcaatacc gccgcccatc
    69841 aagccaccac ctaaaattcc cacgctgttt aatgccgcag gcggtgcatc actaccggga
    69901 tctttcttca cgtccgtact ggcaaaaaag atactacgca aagcttgcga ttgcggcgtc
    69961 atcgccagtt cgccaaaggc gcgagcttca gcgtcatagc cgctgctggt gccctgcgct
    70021 aatcccgttt caacaacctc caggatgcgt tctgtcgccg gataattgcc ttgagttttg
    70081 tgttctgttt tcttgccgac cattttgaac agcagcgcac gacctaacgg ccccgccaga
    70141 atacgctcgc gtacaggtag agggcgggaa gatgggcgat ccttcttcgc cagctcaaca
    70201 gcggcttcca gcaaaatgga gtgcggaaca acgtcatcca ccagccccag ctttaatgcc
    70261 tgtttcgccc gaagttgttt tccggtgaga atcatctcta atgctgtact gacgcctatc
    70321 agacgcggta aacgctgggt gccgcctgaa ccgggtaaca atccaagttg tacttcagac
    70381 aaaccgagca ccgttttagg atcgtcagta caaacgcgac cgtggcacgc cagcgccagc
    70441 tccagcccac cgcccaggca agcgccatga atagccgcga taaccggaat gggcaaagca
    70501 tgaatctccg ccatcaactg ttggccctgc cgcgccagtg cttccgcttc ttgcgccgtt
    70561 ttgcagttgc caatcatgtt gatgtctgct ccggcgataa aattgtccgg tttcgcggag
    70621 ataaacacca cgccgcgcaa ctctttgttt tcacggagtt gcttaataat ggcgcgcacc
    70681 tgcgaggcaa attccgcctt cagggtattc attttctcat ccggtacgtc gatggtgatg
    70741 atagcaatgt tgtccagacg aacattaaga gtaaacgctg atgccatttc cattattccg
    70801 cctccagaac cattgccgcg ccaagcccac cggcagcaca ggcggtaact aaaccaaatc
    70861 caccgccgcg acggcgaagt tcatgcaatg tctgggtaat catccgcgcg ccggtcgccg
    70921 cgaagggatg tccgtaagca atcgaaccgc caagcacgtt aaatttgcta tcgtccactt
    70981 cgccagtggc atgtgcacgc cccagtactt cacgagcaaa acgttcacta cccagcaact
    71041 gaatattcgc cagtgtctga gctgcaaagg cttcgtgcat atcgatcaat gtcagttcgc
    71101 tcatcgtcaa accggcacgt tccagcgcca gcggtgttga ccaggctgga ccgagcaaca
    71161 tgtcctgcca gacatcaatc gcagtaaatg cgtagctgcg cagatacccc agcggcacca
    71221 gccctaattc tttcgcccgg gattcagtca tcaggatcac cgctgccgcg ccatcggtca
    71281 gcggcgtact gtttgccgcc gttaccgttc cgtgtttgcg atcaaacgcc gggcgcagct
    71341 ttgcgtaatc ggcaagcgag gaattaccgc gaatattgtt gtcttcgaca agcggttgtt
    71401 tataaggagg gataaaggca gtcatcacct cttctttgag ttttccgtct gaccatgcct
    71461 gagcggcacg ctgatgcgaa cggtgcgcta atgcatcttg ctgttctcgg gtgatgccgt
    71521 aggttttcgc catttgttcg gcggtgtcgc ccatccgcaa gccagtagaa tattctgcta
    71581 ccgcaggcgg tacgggcatt aagtcgcgca aacgcaggcg agagaagagt ttcaggcgct
    71641 ggctcatggt tcgagctttg ttgacatcaa ccagcacgtg cgccagtttt ttactgacgc
    71701 caattggcaa tacggaagag gaatctgccc caccggcaat ccccgctcgg atagttcccg
    71761 ccatcaggct ttctgcgacg tttgcaaccg cctggaaact ggtagcgcaa gcgcggctga
    71821 cgctgtaagc atcggtatgc acattcattc cggtaccgag aacaatttca cgcgcaatgt
    71881 tgggggcttc aggcatttgt acgacctgac caaagaccag ctgttcaatc acttcagcgg
    71941 ggatctcgct gcgtgccagc agttcgccta ccaccatctt ccctaaatca accgtgggaa
    72001 tgccatgaaa agccgtcgcc tggcgggcaa aaggcgtacg taaaccgcta acaatggcga
    72061 tacgatcgcc ctggcgggta accagtggta aaacctgacc cataacactc ccctgtaaaa
    72121 aaataaataa agtggtctga cctgatcata gtcttaacca tttttttaca tttagccaag
    72181 cggagaaaaa ggaaagtggg agctatgaca cagtgaagac agaagaaaga aaagaaaacg
    72241 cccctgccag caactgacag gggcgtacgt acccgagagg aattaacgca gacccagctg
    72301 gaagatcagc atttcagctt cgcaggcaaa agtgaaatcg atatccaggc gcacaccgtc
    72361 agactcttca gtgaaagtcg gggagatttt gcacggttcg gattccacgc tacgggattt
    72421 ttcagtcagc gccgccagcg tttgttctgc ttctgcgcgg tttgcaaaca cgcggctgta
    72481 agacgcggtg cagtcggagt tgtccataat ggtgccaaca tccatacagc agcaaaccgg
    72541 ggtttcatca gcactacatt tactcatcgt tgatttcctc tgtatgtgca cccaaggtgc
    72601 cagataaacg ttgtggatat tttacgcttc cggaaagtgc tgctccagtt gttaattctg
    72661 caaaatcgga taagtgaccg aaatcacact taaaaatgat ctaaaacaaa attcacccga
    72721 atccgcgagt gcgcaacctc caaattttgc cagctggatc gcgtttctta gatcatattt
    72781 gaaaaaagat ggaaacatac ttgcaacatt ctagctggtc cgacctatac tctcgccact
    72841 ggtctgattt ctaagatgta cctcagaccc tacacttcgc gctcctgtta cagcacgtaa
    72901 catagtttgt ataaaaataa atcattgagg ttatggtcat gagccagaaa accctgttta
    72961 caaagtctgc tctcgcagtc gcagtggcac ttatctccac ccaggcctgg tcggcaggct
    73021 ttcagttaaa cgaattttct tcctctggcc tgggccgggc ttattcaggg gaaggcgcaa
    73081 ttgccgatga tgcaggtaac gttagccgta accccgcatt gattaccatg tttgaccgcc
    73141 cgacattttc tgcgggtgcg gtttatattg acccggatgt gaatatcagc ggaacgtctc
    73201 cgtctggtcg tagcctgaaa gccgataaca tcgcgcctac ggcatgggtt ccgaacatgc
    73261 actttgttgc accgattaac gaccaatttg gttggggcgc ttctattacc tctaactatg
    73321 gtctggcaac agagtttaat gatacttatg caggcggctc tgtcgggggt acaactgacc
    73381 ttgaaaccat gaacctgaac ttaagcggtg cgtatcgctt aaataatgca tggagctttg
    73441 gtcttggttt caacgccatc tacgctcgcg cgaaaattga acgtttcgca ggcgatctgg
    73501 ggcagttggt tgctggtcag attatgcaat ctcctgccgg gcaaactcct caagggcaag
    73561 cattggcagc taccgccaac ggtatcgaca gtaataccaa aatcgctcat ctgaacggta
    73621 accagtgggg ctttggctgg aacgccggaa tcctgtatga actggataaa aataaccgct
    73681 atgcactgac ctaccgttct gaagtgaaaa ttgacttcaa aggtaactac agcagcgatc
    73741 ttaatcgtgc gtttaataac tacggtttgc caattcctac cgcgacaggt ggcgcaacgc
    73801 aatcgggtta tctgacgctg aacctgcctg aaatgtggga agtgtcaggt tataaccgtg
    73861 ttgatccaca gtgggcgatt cactatagcc tggcttacac cagctggagt cagttccagc
    73921 agctgaaagc gacctcaacc agtggcgaca cgctgttcca gaaacatgaa ggctttaaag
    73981 atgcttaccg catcgcgttg ggtaccactt attactacga tgataactgg accttccgta
    74041 ccggtatcgc ctttgatgac agcccagttc cggctcagaa tcgttctatc tccattccgg
    74101 accaggaccg tttctggctg agtgcaggta cgacttacgc atttaataaa gatgcttcag
    74161 tcgacgttgg tgtttcttat atgcacggtc agagcgtgaa aattaacgaa ggcccatacc
    74221 agttcgagtc tgaaggtaaa gcctggctgt tcggtactaa ctttaactac gcgttctgat
    74281 aacgcgttcg cctagataaa gtcacctgct atgcaggtga ctttaactcc cccacttcac
    74341 cgaaagtagt ttcctcattg cttgcctcct gagttttgca aacagcctga tggcagcttg
    74401 ccctttcaaa tcagtaagta gtatccacag acaaacctga agggaaatgc atattttcaa
    74461 gcgttctgct cgtccttctc aaagagttta cttttctgca tttccaggat actcaacccc
    74521 cctggctatt gtgcgcttat acactcaaat taaagatagg ttctaaataa atgagcgttt
    74581 tttgatagtc tatttcatta ggtaatatat atttgtaaca aatcaatcaa catggaataa
    74641 aatcatgcta ccatctattt caatcaacaa taccagcgca gcttacccag aatccatcaa
    74701 tgaaaataac aatgatgaaa ttaatggatt agtacaagag ttcaaaaacc atttttaatg
    74761 gtaaggaagg aataagcacc tgtattaaac atctacttga gcttataaaa aacgccatac
    74821 gagtaaacga cgatccttat agatctaata ttaataattc ctcagttact tatattgata
    74881 ttggctccaa tgatacagac catattacta ttggtatcga caaccaagaa ccaatagaat
    74941 tacctgcgaa ctataaagac aaagaactcg tccgtactat cattaatgac aacatagttg
    75001 agaagactca tgatatcaat aacaaggaaa tgatcttcag cgcattaaaa gaaatatatg
    75061 atggagatcc tggttttatc ttcgataaga tatcacacaa actcagacat acggtaacgg
    75121 aatttgatga gagcgggaaa agcgaaccaa cggacttatt tacctggtac ggtaaagata
    75181 aaaaaggcga ctctctcgct attgtaatta aaaataaaaa cggaaatgat tacttatttc
    75241 tcggttacta cgatcaggac gactaccaca ttcaaagagg aattcgtatt aatggtgata
    75301 gtctcaccca atattgtagt gaaaacgcca ggagtgcttc agcgtggttt gaaagcagta
    75361 aagctatcat ggcagaatca tttgcaactg gttccgatca tcaggttgta aacgagctca
    75421 acggggaaag actgagagaa cctaacgaag tttttaaacg tttaggtcga gcaataagat
    75481 ataattttca agtggacgat gcaaaattta gacgcgataa tgtaaaagaa ataatttcta
    75541 ctttattcgc taacaaagtt gacgttgacc atcctgaaaa taaatacaaa gactttaaga
    75601 atctcgaaga taaagttgaa aaaagacttc aaaatcgcca gacgaaatat caaaatgaaa
    75661 ttaatcaact atctgcacta ggtgttaatt ttgatgatat ttaaaattat ttttccagtt
    75721 ttttgatttt tcaacgcctg aatatcggaa atagtcagtg acaaacatca gtatttttac
    75781 atcattacgt ccgggtcatt gtcggtttat ctccttttac ttgtggtaat tgagggataa
    75841 caacaaaaaa ggtgagtttt gcgactcacc ttttttattt gtttcttatt cagaatcgat
    75901 atcttttaaa tcatcctgaa tcgcttgcgc gttcggattt tcctgcggtt tgagttcgcc
    75961 gccattagcg atgaaatcat gacgctggaa gtacgcttcg cgcaccataa tataaggatc
    76021 ggacgactga cgcagcagac catcggaatc cagcagctga gcgcgggttt cgatcccttc
    76081 aagcgtccat ttacccacag acatcggcca ggtcagccag gaaagaaccg ggtaaagagc
    76141 atccgccata tcaccaccgt catcacgcag cgtgaagcta ccgtagaacg gtaactgaac
    76201 gtaaggccca taacccacgc cataatgacc aagcgtacta ccgaagcggt gaggttcagt
    76261 ccgttgcagt ttcgggttcg ccatccctgc aacatcaata aagccgccca tccccaaaat
    76321 ggtgttcagg aaaaagcggg taaagtggac catcccctga taagggtcgc cctgcaagaa
    76381 gtagttaacc atcaccgcag gttcttcaag gttgccagta aagttgctca aaccattacg
    76441 cgccggttgc ggaacataat cacgccaggc gacagcgacc ggtcgaacaa tatacgggtc
    76501 taatacgttg aagttgaagt tgtacatggt gcggttgaac ccttctaacg ggtcagaacg
    76561 cccttgctga tctgtaccgg aactcgcaca ccccaccaga agcgtagttc ccagagcaag
    76621 cgccgacagg cgaagcttca taaatgtctc cctgtttttt tatggcttat gcagtttgcc
    76681 atccatgacg gaacgatacc gtatccgcct gtttaggtgt gggcgattgt aacagcacgt
    76741 caactgatgt ccagacgccc tgatttgctg atttgatcat agcctggtaa tcgccgccct
    76801 gtaggctact tgattctata gaaacagaaa aaggcaaacg cagccttttc tacattttca
    76861 gagtaactcc cgccagttgc gagcaaaaaa gccgctacgc tttagctata cgtgctaatt
    76921 caagagaaga gaccatggac aacgacaaaa ttgatcaaca cagcgacgaa attgaagttg
    76981 agagcgaaga aaaagagcgc ggcaaaaaaa tagaaataga tgaagaccga ctcccctccc
    77041 gagcgatggc aattcatgag catatccgcc aggatggtga aaaagagcta gaacgcgacg
    77101 caatggcgct actgtggtca gccattgcgg cgggtctgtc gatgggcgct tcgttactgg
    77161 caaaagggat atttcatgcc gaactggaag gtgtgccggg cagcttcttg ctggagaatc
    77221 tcggttatac ctttggtttt attatcgtca taatggcccg ccagcaatta tttaccgaaa
    77281 acaccgtgac tgcggtacta cccgtcatgc aaaaaccgac aatgagcaac gttggcttac
    77341 ttatgcggtt atggggagtc gtgctgctgg gtaatattct cgggactggt attgctgcgt
    77401 gggcatttga atatatgcct atcttcaatg aagaaactcg cgatgcattt gtcaaaatcg
    77461 gcatggatgt gatgaagaac acccccagcg agatgtttgc caacgcgatc atttccggct
    77521 ggctgatcgc cactatggta tggatgtttc ctgcagcagg tgcggcaaag attgtggtga
    77581 ttatattgat gacctggctt attgccctgg gtgacaccac ccatatcgtg gtcggttctg
    77641 ttgaaatcct ctatctggtg tttaacggta cgctgcactg gagcgatttc atctggccct
    77701 tcgcactacc tactttagcg gggaacatct gcggcggcac ctttatcttc gcgttaatga
    77761 gtcatgcaca gattcgtaac gacatgagca acaagcgtaa agcagaagca cgccaaaaag
    77821 cagaacgtgc ggaaaacatt aagaaaaatg ataaaaaccc agcataaatg gcgagggttt
    77881 aagcaatcga gcggcagcgt acttaccccg cagtccatta gcgggtatac tcgccgcatt
    77941 gtcctcttag ttaaatggat ataacgagcc cctcctaagg gctaattgca ggttcgattc
    78001 ctgcagggga caccatttat cagttcgctc ccatccgtac cagtccgcaa aatcccctga
    78061 atatcaagcc ttccgtatat tcacagttcg tcatggttcg cgtcagatcg ttgacagccg
    78121 cactccatga cgggtaaaaa gtggataaaa taattttacc caccggattt ttacccatgc
    78181 tcaccgttaa gcagattgaa gcagcaaagc cgaaagaaaa accataccgc cttctcgatg
    78241 gtaatggcct gtacctttat gtccctgtat ccgggaaaaa ggtatggcag cttcgctaca
    78301 agattgacgg taaggagaaa atcctgaccg tcggaaaata tccgcttatg actttgcagg
    78361 aggcaaggga taaagcatgg actgcgagga aagacatctc ggctggcatc gatccggtaa
    78421 aggcgaaaaa ggcttcgtct aacaacaatt catttagtgc gatttacaag gaatggtacg
    78481 aacacaagaa gcaagtctgg tcagtagggt atgcgactga acttgcaaaa atgtttgatg
    78541 acgacatttt acccatcatc ggcggccttg agattcagga tattgagccg atgcaactgc
    78601 tggaagtaat ccgcagattt gaagatcgcg gtgcaa
//
LOCUS       DAEEUE010000004        75608 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.004, whole genome shotgun sequence.
ACCESSION   DAEEUE010000004 DAEEUE010000000
VERSION     DAEEUE010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75608
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.004"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(435..641)
                     /locus_tag="KLK84_000269"
     CDS             complement(435..641)
                     /locus_tag="KLK84_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HBE2007753.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            954..1043
                     /gene="kdpF"
                     /locus_tag="KLK84_000270"
     CDS             954..1043
                     /gene="kdpF"
                     /locus_tag="KLK84_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBE2007754.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1043..2716
                     /gene="kdpA"
                     /locus_tag="KLK84_000271"
     CDS             1043..2716
                     /gene="kdpA"
                     /locus_tag="KLK84_000271"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBE2007755.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2739..4786
                     /gene="kdpB"
                     /locus_tag="KLK84_000272"
                     /pseudo
     CDS             2739..4786
                     /gene="kdpB"
                     /locus_tag="KLK84_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4795..5367
                     /gene="kdpC"
                     /locus_tag="KLK84_000273"
     CDS             4795..5367
                     /gene="kdpC"
                     /locus_tag="KLK84_000273"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBE2007756.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5360..8040
                     /gene="kdpD"
                     /locus_tag="KLK84_000274"
                     /pseudo
     CDS             5360..8040
                     /gene="kdpD"
                     /locus_tag="KLK84_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            8037..8714
                     /gene="kdpE"
                     /locus_tag="KLK84_000275"
     CDS             8037..8714
                     /gene="kdpE"
                     /locus_tag="KLK84_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HBE2007757.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSGVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8989..9093
                     /locus_tag="KLK84_000276"
     CDS             8989..9093
                     /locus_tag="KLK84_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HBE2007758.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9404..11592
                     /gene="speF"
                     /locus_tag="KLK84_000277"
                     /pseudo
     CDS             9404..11592
                     /gene="speF"
                     /locus_tag="KLK84_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11589..12908
                     /gene="potE"
                     /locus_tag="KLK84_000278"
     CDS             11589..12908
                     /gene="potE"
                     /locus_tag="KLK84_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HBE2007759.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12964..14604)
                     /gene="pgm"
                     /locus_tag="KLK84_000279"
     CDS             complement(12964..14604)
                     /gene="pgm"
                     /locus_tag="KLK84_000279"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HBE2007760.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14630..15175)
                     /gene="seqA"
                     /locus_tag="KLK84_000280"
     CDS             complement(14630..15175)
                     /gene="seqA"
                     /locus_tag="KLK84_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HBE2007761.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15360..16124
                     /gene="ybfF"
                     /locus_tag="KLK84_000281"
     CDS             15360..16124
                     /gene="ybfF"
                     /locus_tag="KLK84_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBE2007762.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16264..16557
                     /gene="ybfE"
                     /locus_tag="KLK84_000282"
     CDS             16264..16557
                     /gene="ybfE"
                     /locus_tag="KLK84_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HBE2007763.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16697..17227
                     /gene="fldA"
                     /locus_tag="KLK84_000283"
     CDS             16697..17227
                     /gene="fldA"
                     /locus_tag="KLK84_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HBE2007764.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17437..17523
                     /gene="uof"
                     /locus_tag="KLK84_000284"
     CDS             17437..17523
                     /gene="uof"
                     /locus_tag="KLK84_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HBE2007765.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17516..17962
                     /gene="fur"
                     /locus_tag="KLK84_000285"
     CDS             17516..17962
                     /gene="fur"
                     /locus_tag="KLK84_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HBE2007766.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18046..18372)
                     /locus_tag="KLK84_000286"
     CDS             complement(18046..18372)
                     /locus_tag="KLK84_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2007767.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18409..19815)
                     /gene="chiP"
                     /locus_tag="KLK84_000287"
     CDS             complement(18409..19815)
                     /gene="chiP"
                     /locus_tag="KLK84_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HBE2007768.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(20394..22058)
                     /gene="glnS"
                     /locus_tag="KLK84_000288"
     CDS             complement(20394..22058)
                     /gene="glnS"
                     /locus_tag="KLK84_000288"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HBE2007769.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22261..24207)
                     /gene="nagE"
                     /locus_tag="KLK84_000289"
     CDS             complement(22261..24207)
                     /gene="nagE"
                     /locus_tag="KLK84_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HBE2007770.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            24540..25340
                     /gene="nagB"
                     /locus_tag="KLK84_000290"
     CDS             24540..25340
                     /gene="nagB"
                     /locus_tag="KLK84_000290"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBE2007771.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRLFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25400..26548
                     /gene="nagA"
                     /locus_tag="KLK84_000291"
     CDS             25400..26548
                     /gene="nagA"
                     /locus_tag="KLK84_000291"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBE2007772.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            26557..27777
                     /gene="nagC"
                     /locus_tag="KLK84_000292"
                     /gene_synonym="nagR"
     CDS             26557..27777
                     /gene="nagC"
                     /locus_tag="KLK84_000292"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HBE2007773.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27825..28577
                     /gene="nagD"
                     /locus_tag="KLK84_000293"
     CDS             27825..28577
                     /gene="nagD"
                     /locus_tag="KLK84_000293"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HBE2007774.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            28974..30638
                     /gene="asnB"
                     /locus_tag="KLK84_000294"
     CDS             28974..30638
                     /gene="asnB"
                     /locus_tag="KLK84_000294"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HBE2007775.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            31017..31093
                     /locus_tag="KLK84_000295"
     tRNA            31017..31093
                     /locus_tag="KLK84_000295"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31051..31053,aa:Met,seq:cat)
     gene            31102..31186
                     /locus_tag="KLK84_000296"
     tRNA            31102..31186
                     /locus_tag="KLK84_000296"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31136..31138,aa:Leu,seq:tag)
     gene            31210..31284
                     /locus_tag="KLK84_000297"
     tRNA            31210..31284
                     /locus_tag="KLK84_000297"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31242..31244,aa:Gln,seq:ttg)
     gene            31319..31393
                     /locus_tag="KLK84_000298"
     tRNA            31319..31393
                     /locus_tag="KLK84_000298"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31351..31353,aa:Gln,seq:ttg)
     gene            31409..31485
                     /locus_tag="KLK84_000299"
     tRNA            31409..31485
                     /locus_tag="KLK84_000299"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31443..31445,aa:Met,seq:cat)
     gene            31534..31608
                     /locus_tag="KLK84_000300"
     tRNA            31534..31608
                     /locus_tag="KLK84_000300"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31566..31568,aa:Gln,seq:ctg)
     gene            31646..31720
                     /locus_tag="KLK84_000301"
     tRNA            31646..31720
                     /locus_tag="KLK84_000301"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31678..31680,aa:Gln,seq:ctg)
     gene            complement(31874..33049)
                     /gene="ubiF"
                     /locus_tag="KLK84_000302"
     CDS             complement(31874..33049)
                     /gene="ubiF"
                     /locus_tag="KLK84_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HBE2007776.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            33195..34619
                     /gene="miaB"
                     /locus_tag="KLK84_000303"
     CDS             33195..34619
                     /gene="miaB"
                     /locus_tag="KLK84_000303"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBE2007777.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            34772..35812
                     /locus_tag="KLK84_000304"
     CDS             34772..35812
                     /locus_tag="KLK84_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBE2007778.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            35809..36276
                     /gene="ybeY"
                     /locus_tag="KLK84_000305"
     CDS             35809..36276
                     /gene="ybeY"
                     /locus_tag="KLK84_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBE2007779.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            36366..37244
                     /gene="corC"
                     /locus_tag="KLK84_000306"
     CDS             36366..37244
                     /gene="corC"
                     /locus_tag="KLK84_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HBE2007780.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            37269..38807
                     /gene="lnt"
                     /locus_tag="KLK84_000307"
     CDS             37269..38807
                     /gene="lnt"
                     /locus_tag="KLK84_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HBE2007781.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            39204..40112
                     /gene="gltI"
                     /locus_tag="KLK84_000308"
     CDS             39204..40112
                     /gene="gltI"
                     /locus_tag="KLK84_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HBE2007782.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            40282..41022
                     /gene="gltJ"
                     /locus_tag="KLK84_000309"
     CDS             40282..41022
                     /gene="gltJ"
                     /locus_tag="KLK84_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HBE2007783.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            41022..41696
                     /gene="gltK"
                     /locus_tag="KLK84_000310"
     CDS             41022..41696
                     /gene="gltK"
                     /locus_tag="KLK84_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HBE2007784.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            41696..42421
                     /gene="gltL"
                     /locus_tag="KLK84_000311"
     CDS             41696..42421
                     /gene="gltL"
                     /locus_tag="KLK84_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HBE2007785.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            42539..43474
                     /gene="rihA"
                     /locus_tag="KLK84_000312"
     CDS             42539..43474
                     /gene="rihA"
                     /locus_tag="KLK84_000312"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HBE2007786.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            43558..45219
                     /gene="hscC"
                     /locus_tag="KLK84_000313"
                     /pseudo
     CDS             43558..45219
                     /gene="hscC"
                     /locus_tag="KLK84_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(45279..46730)
                     /gene="djlC"
                     /locus_tag="KLK84_000314"
     CDS             complement(45279..46730)
                     /gene="djlC"
                     /locus_tag="KLK84_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HBE2007787.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(46727..47434)
                     /locus_tag="KLK84_000315"
     CDS             complement(46727..47434)
                     /locus_tag="KLK84_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBE2007788.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            47536..48090
                     /locus_tag="KLK84_000316"
     CDS             47536..48090
                     /locus_tag="KLK84_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBE2007789.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLRWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(48100..49527)
                     /locus_tag="KLK84_000317"
     CDS             complement(48100..49527)
                     /locus_tag="KLK84_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HBE2007790.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLSSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHEGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(49524..50231)
                     /locus_tag="KLK84_000318"
     CDS             complement(49524..50231)
                     /locus_tag="KLK84_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBE2007791.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            50395..51372
                     /locus_tag="KLK84_000319"
     CDS             50395..51372
                     /locus_tag="KLK84_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HBE2007792.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(51442..51924)
                     /locus_tag="KLK84_000320"
     CDS             complement(51442..51924)
                     /locus_tag="KLK84_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HBE2007793.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            52159..54741
                     /gene="leuS"
                     /locus_tag="KLK84_000321"
     CDS             52159..54741
                     /gene="leuS"
                     /locus_tag="KLK84_000321"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBE2007794.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            54756..55337
                     /gene="lptE"
                     /locus_tag="KLK84_000322"
     CDS             54756..55337
                     /gene="lptE"
                     /locus_tag="KLK84_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HBE2007795.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            55337..56368
                     /gene="holA"
                     /locus_tag="KLK84_000323"
     CDS             55337..56368
                     /gene="holA"
                     /locus_tag="KLK84_000323"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBE2007796.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            56370..57011
                     /gene="nadD"
                     /locus_tag="KLK84_000324"
     CDS             56370..57011
                     /gene="nadD"
                     /locus_tag="KLK84_000324"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBE2007797.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            57035..57646
                     /locus_tag="KLK84_000325"
     CDS             57035..57646
                     /locus_tag="KLK84_000325"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HBE2007798.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            57906..58223
                     /gene="rsfS"
                     /locus_tag="KLK84_000326"
                     /gene_synonym="rsfA"
     CDS             57906..58223
                     /gene="rsfS"
                     /locus_tag="KLK84_000326"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBE2007799.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            58227..58694
                     /gene="rlmH"
                     /locus_tag="KLK84_000327"
     CDS             58227..58694
                     /gene="rlmH"
                     /locus_tag="KLK84_000327"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBE2007800.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            58725..60626
                     /gene="mrdA"
                     /locus_tag="KLK84_000328"
     CDS             58725..60626
                     /gene="mrdA"
                     /locus_tag="KLK84_000328"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HBE2007801.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            60629..61741
                     /gene="mrdB"
                     /locus_tag="KLK84_000329"
                     /gene_synonym="rodA"
     CDS             60629..61741
                     /gene="mrdB"
                     /locus_tag="KLK84_000329"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HBE2007802.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGMWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            61752..62840
                     /gene="rlpA"
                     /locus_tag="KLK84_000330"
     CDS             61752..62840
                     /gene="rlpA"
                     /locus_tag="KLK84_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HBE2007803.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPSVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            62979..64190
                     /gene="dacA"
                     /locus_tag="KLK84_000331"
     CDS             62979..64190
                     /gene="dacA"
                     /locus_tag="KLK84_000331"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HBE2007804.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            64302..64565
                     /locus_tag="KLK84_000332"
     CDS             64302..64565
                     /locus_tag="KLK84_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HBE2007805.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            64666..65307
                     /gene="lipB"
                     /locus_tag="KLK84_000333"
     CDS             64666..65307
                     /gene="lipB"
                     /locus_tag="KLK84_000333"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HBE2007806.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            65566..66519
                     /locus_tag="KLK84_000334"
     CDS             65566..66519
                     /locus_tag="KLK84_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBE2007807.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            66728..67693
                     /gene="lipA"
                     /locus_tag="KLK84_000335"
     CDS             66728..67693
                     /gene="lipA"
                     /locus_tag="KLK84_000335"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HBE2007808.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(67794..67997)
                     /gene="tatE"
                     /locus_tag="KLK84_000336"
     CDS             complement(67794..67997)
                     /gene="tatE"
                     /locus_tag="KLK84_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HBE2007809.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(68126..68914)
                     /locus_tag="KLK84_000337"
     CDS             complement(68126..68914)
                     /locus_tag="KLK84_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HBE2007810.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            69007..69390
                     /gene="crcB"
                     /locus_tag="KLK84_000338"
     CDS             69007..69390
                     /gene="crcB"
                     /locus_tag="KLK84_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HBE2007811.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(69444..69653)
                     /gene="cspE"
                     /locus_tag="KLK84_000339"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(69444..69653)
                     /gene="cspE"
                     /locus_tag="KLK84_000339"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBE2007812.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(69828..70388)
                     /gene="pagP"
                     /locus_tag="KLK84_000340"
     CDS             complement(69828..70388)
                     /gene="pagP"
                     /locus_tag="KLK84_000340"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HBE2007813.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            70976..72361
                     /gene="dcuC"
                     /locus_tag="KLK84_000341"
     CDS             70976..72361
                     /gene="dcuC"
                     /locus_tag="KLK84_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBE2007814.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTDK"
     gene            complement(72402..72743)
                     /locus_tag="KLK84_000342"
                     /pseudo
     CDS             complement(72402..72743)
                     /locus_tag="KLK84_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(73081..73590)
                     /locus_tag="KLK84_000343"
     CDS             complement(73081..73590)
                     /locus_tag="KLK84_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBE2007815.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            73753..73998
                     /locus_tag="KLK84_000344"
     CDS             73753..73998
                     /locus_tag="KLK84_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE2007816.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            73973..75604
                     /locus_tag="KLK84_000345"
                     /pseudo
     CDS             73973..75604
                     /locus_tag="KLK84_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
BASE COUNT        18609 a        18986 c        19205 g        18808 t
ORIGIN      
        1 cataacatct tattggatta gatatgttaa caattaatta ttcttaatat atcaataaag
       61 tatatattac tctctttact atctgttttt attgcggtag cgatactaat aacaaaattc
      121 ggaatttatt tagatgctgg aattagaaaa agggatgacg gctttctcac cttaccctga
      181 agtttcacga aagtgtgtaa ggaagtatgc aatctaactg gagatttctg atgatctagt
      241 cgcttagagc gacaatgaag gtgaccgcat cctgggtagc cctatgaaag gatactattg
      301 aaacgtgatg ctaaaacagt gatgaaatga ataatacgat ggaaacgtct tccatgttaa
      361 ttatcatgga agacgttgat gttgaacacc taaaccagcc ccctgatacg aggctggctt
      421 aagcgcgcag gcaatcagta aaaatcaccg gttgcctttt cggcctgatc catccacacc
      481 ggtttatcgc tggttttcga ccagacgcgg tgcaggtagc tgtaaaagcg ggcgcggtct
      541 ttccagaaaa gcatgaaagg cagcgccaga acgcccgctg caaccgcata tgtacggcgt
      601 aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc ccccttttgt
      661 atctacccgg tgaatagcac cggaaaaatg aatttgttta tctgatgaaa atagtaccgc
      721 cttttgtgta attttactac tcatccgacc acttattttt gcttattgat ggtttattta
      781 cattcacact gtaattaaat tacacaaaag ttaaattaat actaaccatc agttaaatca
      841 tggcctttgc catttttata ctttttttac accccgtccg cagatttttg cgaaatcttt
      901 gcagccagaa ttctactctt ccggtatcac ttttaggcca ctggaggtgc actgtgagtg
      961 caggcgtgat agccggcgtg ttgctggtgt ttttattact gggttatttg gtttatgccc
     1021 tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgattg ccacgttttt
     1081 actggtatta atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc tgattaatga
     1141 cattcctctt ccaggtacag cgggcgttga acgtatactc tttcgtttac ctggcgtctc
     1201 tgaccatgag atgaactgga agcaatatct ttgtgccatt ctcggcctga acatgctggg
     1261 gctggcggtg ctgtttttta tgttgctcgg tcagcactat ctgccgctta atccacagca
     1321 gttgccgggg ctgtcgtggg atctggcgct gaataccgcc gtcagctttg tcacgaatac
     1381 caactggcaa tcttatagcg gtgaaaccac gttgagctat ttcagccaga tggcaggctt
     1441 aacggtacaa aactttcttt ctgccgccag cgggattgcg gtgatttttg ccttcatccg
     1501 tgcgtttact cgccagagca tgagcacact cgggaatgcc tgggtcgatc tgctacgcat
     1561 cacgttatgg gtgctagtcc ctgtggcgtt gttgatcgca ctgtttttta ttcaacaagg
     1621 tgcgcagcaa aactttctgc cctatcaggc agtgaatacc gttgaaggag cgcaacagct
     1681 gttacccatg ggaccagtag cttctcagga agcgatcaag atgctcggta ctaacggcgg
     1741 tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt
     1801 cgtgcagatg ctggcgatct tcttgatccc aacagcgctg tgctttgcct ttggtgaagt
     1861 gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat
     1921 ctgcgtaggc gtggttatgt gggcagaagt tcagggtaat cctcatctgc tggcgctcgg
     1981 cgcagacagc agcatcaata tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag
     2041 cctgtttgcg gtcgtgacta cggcggcgtc ctgtggcgcg gtgattgcga tgcatgattc
     2101 gtttaccgct ctcggcggca tggtgccgat gtggctaatg caaattggtg aagtggtgtt
     2161 cggcggtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg cggtgtttat
     2221 tgccgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatag acgtacgcga
     2281 gatgaaactg actgcactgg cgattctggt tactccaaca ctggtactga tgggcgcggc
     2341 gttggcgatg atgaccgacg ccggacgtag cgccatgctt aaccctagcc cgcatggttt
     2401 tagcgaagtg ctgtacgccg tgtcgtccgc cgctaacaac aacggcagcg cctttgccgg
     2461 attaagcgcc aactctccgt tctggaactg tttactggcg ttctgcatgt ttgtcggtcg
     2521 cttcggggtg attatcccgg tgatggcaat tgccggttcg ctggtgagta aaaagagcca
     2581 acccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc tgttaatcgg
     2641 caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg gtccggtggc
     2701 ggaatatctc tcctgatgat attgagtgag cactgaatat gagtcgtaaa caactggcgc
     2761 tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc
     2821 aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat cggcagtctg ctgaccacct
     2881 gtattagcat cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt agcgcggcaa
     2941 ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg ctggcagaag
     3001 gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc tttgcccgca
     3061 agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat caactgcgta
     3121 aaggtgacat cgtgctggta gaagctggcg atattatccc ctgcgatggt gaagtcattg
     3181 aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg
     3241 aatccggcgg cgattttgcc tccgtcaccg gcggcacgcg tattctttct gactggctgg
     3301 tgattgagtg tagcgttaac cccggtgaga catttctgga tcggatgatc gcgatggtgg
     3361 aaggcgcaca gcgacgcaaa acaccgaacg agattgccct gactatcctg ctgattgctc
     3421 tgaccatcgt ctttttactg gcaaccgcca cgctgtggcc gttttccgcg tggggcggta
     3481 atgccgtgag tgtaacggta ctggtggcgc tgctggtctg tctgatccca accactattg
     3541 gtggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga
     3601 ttgccaccag cggacgtgca gttgaagcgg caggtgacgt tgacgttctg ctactggata
     3661 aaaccggcac catcacactc ggtaaccgtc aggcgtcgga gtttatcccc gcgcagggcg
     3721 tggatgaaaa aacgctggct gacgccgcac aactggcttc gctggctgat gaaacgccgg
     3781 aaggtcgcag tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc
     3841 agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca
     3901 tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat attgaggcta
     3961 acggcggtca cttccctgcc gatgttgatc aaaaagtcga tcaggttgcg cgtcaaggag
     4021 ccacgctgct ggtggtggtg gaaggttctc gtgtgctggg cgttattgcg ctgaaagata
     4081 tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg caaatgggca ttaaaacggt
     4141 gatgattacc ggcgataacc gtctgactgc cgccgcgata gctgcggaag cgggtgtcga
     4201 tgattttctc gccgaagcga caccggaggc caagctggca ttgatccgtc agtatcaggc
     4261 ggaaggtcgt ttggtagcga tgaccggcga cagcaccaac gatgctacgg cgctggcgca
     4321 ggcagatgtc gcggtggcga tgaactcagg cacccaggcg gctaaagagg cgggcaatat
     4381 ggtggatctc gactctaacc cgaccaagtt gatcgaggtg gtgcacattg gcaaacagat
     4441 gctgatgacc cgtggctcgc tgaccacctt cagcattgcc aacgatgtgg cgaaatactt
     4501 cgccattatt ccggcggcgt tcgcggcaac atatccgcag ttaaatgcgc tgaacatcat
     4561 gcgcctgcat tcgcccgact ccgccattct cagtgcggtc attttcaacg ccttgattat
     4621 cgtctttttg attcctctgg cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc
     4681 catgttgcgc cgtaacttat ggatttacgg tctgggtggg ctgctggtgc cgtttattgg
     4741 tatcaaagtc attgatttac tgctaaccgt ttgtagtctg gtgtgaggtt taccatgagt
     4801 ggattacgtc cggcattatc gacatttctc tttctgttat tgattactgg cggcgtttac
     4861 ccgctgctga ccaccgcact ggggcaatgg tggtatccct ggcaggccaa cggttcgttg
     4921 attcgtgaag gtgatacggt gcgcggttcg gcattaatcg ggcaaaattt taccggcaac
     4981 ggctattttc atggtcgccc gtcggcaaca gcagaaatgc cctataatcc acaggcttct
     5041 ggcgggagca atctggcagt cagtaacccg gagctggata aacagatagc cgcacgcgtt
     5101 gccgcattac gggccgctaa cccggacgcc agcacgaacg ttccggttga actggtgacg
     5161 gcgtcggcaa gcggactgga caataacatc accccgcaag cggcggcctg gcagatccca
     5221 cgcatcgcaa aagcgcgtaa tctcagcgtt gaacagctca cgcagctgat cgcaaaatac
     5281 agccaacaac cgctggtgaa atatatcggc cagccggtcg tcaacattgt cgaactcaat
     5341 ctggcactgg ataagcttga tgaataacga acccttacgt cccgaccccg accgtctgct
     5401 ggaacaaact gccgcgccgc atcgggggaa gctgaaagtt ttcttcagtg cctgtgcagg
     5461 cgtcgggaag acctgggcga tgctggcaga agcccagcga ctgcgggcgc aagggctgga
     5521 tattgtggtt ggtgtggtag aaacccatgg gcggaaagat accgccgcca tgcttgaagg
     5581 gctggctgtt ctgcctctga aacgccaggc gtaccgtggg cggcatatca gcgagtttga
     5641 tctcgatgcc gccctcgccc gccgtccggc gctgatctta atggacgaac tggcgcacag
     5701 taatgcgcca ggttctcgtc atcctaaacg ctggcaggat atcgaagaac tgctggaagc
     5761 tggcattgat gttttcacaa ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt
     5821 cagcggcgtc accggaattc aggtacggga aaccgtgccc gatccttttt tcgatgccgc
     5881 cgacgacgtg gtgctggtgg acttgccccc ggacgatctg cgccagcggc tgaaagaagg
     5941 caaagtctat attgccgggc aggcggagcg cgccattgaa cattttttcc gcaaaggcaa
     6001 tctgatcgcc ctgcgcgaac tggcactgcg ccgtactgcc gatcgcgttg atgagcaaat
     6061 gcgcgcctgg cgggggcatc ctggcgaaga gaaagtctgg cacacgcgcg acgcgatcct
     6121 tttatgcatc ggccataaca ccggcagcga aaactggtcc gcgcggcggc gcggctgacg
     6181 tcacggctgg ggagcgtctg gcacgcggtg tatgttgaaa cccctgccct gcatcgctta
     6241 ccggagaaaa aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg
     6301 gagacggcaa cactttctga tccagcggaa gagaaagcgg tagtgcgtta tgcccgtgaa
     6361 cataatctcg gcaagattat tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa
     6421 acatttgctg accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt
     6481 gatgaaccac ccgcccgcac gattaacaac gcgccggata gccgctcatt taaagacaag
     6541 tggcgggtac agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta
     6601 attgccatgc agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt
     6661 ggcgtggtgg tggcgctatt ttatggacgc tggccttcgg tggttgccac cgtcattaat
     6721 gtggtgagtt tcgatctctt ttttatcgcc ccacgcggca cgctcgccgt ctctgatgtg
     6781 caatatctgc tgaccttcgc ggtgatgtta accgtcgggc tggtgatcgg gaaccttact
     6841 gctggcgtgc gttatcaggc gcgggtagcc cgttaccgcg agcaacgcac acggcactta
     6901 tatgaaatgt cgaaagctct ggcggtgggc cgcagtccgc aggacatcgc cgccaccagc
     6961 gaacaattta ttgcctccac gtttcatgcc cgcagtcagg tgttgttgcc cgatgacaac
     7021 ggtaaattgc agccgttaac acatccgcaa ggagttacgc catgggacga tgccatcgcg
     7081 cagtggagtt ttgataaagg cctgcctgcg ggcgcgggca ccgacacgtt acccggtgta
     7141 ccgtaccaga ttttgccgct aaaaagcggc gagaaaacct acgggctggt ggtggtggag
     7201 ccggggaatc tgcgccagtt gatgatcccg gaacagcagc gcctgctgga gacttttacg
     7261 ctgttagtcg ccaatgccct tgagcggctg acgctaaccg ccagcgaaga acaggcgcgg
     7321 atggcaagcg aacgtgaaca gatccgcaac gccctgctgg cagcgctttc gcatgattta
     7381 cgcacgccgc ttacggtgct gtttggtcag gcagaaatct taacgctcga tctggcaagc
     7441 gaaggatcac cccacgcccg ccaggccagc gagattcgtc agcatgtgct gaacactacc
     7501 cgactggtga ataatctact ggatatggcg cgaattcagt ccggcggctt taatttgaag
     7561 aaagagtggt taacgctgga agaagtagtc ggcagcgcgc tgcaaatgcc ggaaccgggt
     7621 ttatcgtcgc ccatcaatct ttctctgcca gaaccgctga ccttaatcca cgttgacggg
     7681 ctactctttg aacgggtgct gattaatctg ctggagaacg cggtgaaata tgcgggtgcg
     7741 caggccgaaa ttggtatcga tgcccacgtt gagggcgaaa atctacaact ggatgtctgg
     7801 gataacggcc ccggtcttcc gccaggccag gagcagacga tatttgataa gtttgctcgc
     7861 gggaataaag agtcggcggt gccgggtgtc gggcttgggt tggcaatttg tcgggcgatt
     7921 gtggatgtac acggcggcac cattaccgcg ttcaaccgac cggaaggtgg tgcctgtttt
     7981 cgtgttacac ttccccagca aaccgctcct gaacttgaag actttcatga ggatatgtga
     8041 caaacgttct gattgttgaa gatgaacagg ctattcgtcg ctttctgcgc acggcgctgg
     8101 agggcgacgg gatgcgcgtc tttgaggccg aaactctgca acgcggcttg ctggaagcgg
     8161 caacccgtaa gccagatttg attattctcg atctcggcct gcccgatggt gatgggattg
     8221 agtttatccg cgacctgcgc cagtggagcc cggtgccggt gattgtgctt tccgcacgca
     8281 gcgaagagag cgacaagatc gccgcgctgg atgccggagc ggatgattat ctgagtaagc
     8341 cgtttggtat tggcgaattg caggcccgtc tgcgcgtcgc tttacgccgc cactccgcca
     8401 ccgccgcgcc cgatccgctg gtgaaatttt ccggtgttac cgtcgattta gccgcccgcg
     8461 tgattcaccg gggtgaggaa gaggtgcatc tcacaccaat tgagttccgc ctgctggcgg
     8521 tgctgctcaa caatgccgga aaagtactca cccagcgcca gctccttaac caggtgtggg
     8581 ggccaaacgc ggtcgaacac agtcactatt tgcgtattta tatgggacat ctgcgacaaa
     8641 aactggaaca ggatccggcc cggcctcgcc acttcattac tgaaaccggt attgggtatc
     8701 ggtttatgct ttgaattgtt attcaataaa ttcgcttaaa tagtgattaa tttattatta
     8761 aacctgcgcc gaatattatt atttggcagg tttaatttct taacgcaaat ataaaacacg
     8821 aaattacaac atttaagtaa caaaacagca tctaatatgg cgatattcat aattaatgat
     8881 ttattatctg gggtatgatc gttttttgtt gatcttcttc acagattata gccatttcat
     8941 ggatagaata actctacctt caactgacac agcaagcggt aaaggtaaat ggaaaataat
     9001 aaccgcttaa tgcctcatat aaggcggaca acccatatca tgaagtttgc ccatcgtaat
     9061 agttttgact ttcatttctt taatgcccgc tagtcttctg actaaagggc accccaacgt
     9121 acaggtctcc ctgactttaa gcattacagg ttaatacctg tattcctcgg tgctcatata
     9181 ctgctacccc ttttaaactc taaataattc gagtcgcagc acttgcaact tgaggtatga
     9241 cgagtatagc cagttaccgg gctggtctgg gttattgcat ctgcaaaaag caaactactg
     9301 atttatttat cagcggtgga gctttgcttt ttttccgacg tgatcgattt ctcctttgag
     9361 aaattgagga cctgctatta cctgaaataa agagatgaat aaaatgtcag aattaaaaat
     9421 tgccgttagc cgttcttgcc cggattgttt ttccactcat cgtgcatgcg tgaatattga
     9481 cgaaagtaat tatattgacg ttgccgccat tattttatca gtcaatgatg ttgaacgtgg
     9541 aaaactcgat aaaatagacg ctactggcta tggcattcct gtttttattg caacggaaaa
     9601 tgaagaacgt gtaccagcag aatatctgcc acgtatttct ggtgtcttcg agcattgcga
     9661 atcacggaaa gagttttatg gacgtcagtt agaaaccgct gctagccatt atgaaacgca
     9721 actgcgcccg cccttcttcc gcgcactggt cgattatgtc aatcagggca atagcgcttt
     9781 tgactgcccg ggtcatcagg gaggtgagtt tttccgtcgt catccggcag ggaatcagtt
     9841 cgtggagtac ttcggagaaa cgctgttccg ttccgactta tgcaacgcag acgtagcaat
     9901 gggcgatctg ctgattcacg aaggcgcgcc atgcattgca cagcaacatg cggcaaaagt
     9961 gtttaatgcc gataaaacat acttcgttct aaatggcact tcatcttcta acaaagtggt
    10021 tttaaacgcc ctgctaacac cgggtgatct ggtgctgttt gatcgcaata accacaaatc
    10081 taaccaccac ggagcgttgc tacaggctgg tgcaacaccg gtttatctgg aaacggcacg
    10141 taacccgtat ggctttatcg gtggcattga tgcgcactgt tttgaagaaa gttacctgcg
    10201 tgagctgatc gcggaagtcg caccgcagcg ggcaaaagag gctcgtcctt tccgcctcgc
    10261 tgtgattcag ttaggcacct acgacggtac gatttataac gcccgccagg tggtggataa
    10321 aattggtcat ctgtgtgact acattctgtt tgactccgca tgggttggct atgagcaatt
    10381 tatcccgatg atggctgact gttcgccgct gttgctggat cttaatgaga acgatccggg
    10441 tattctagtt acgcaatccg tacataaaca gcaggctggt ttttctcaaa cttcacaaat
    10501 ccataaaaaa gacagccaca tcaaagggca acagcgttat gtaccgcaca aacgcatgaa
    10561 caacgccttt atgatgcacg cctccaccag cccgttctat ccgctgtttg ccgcactgga
    10621 tatcaacgcc aaaatgcatg aaggtgtcag cggtcgtaat atgtggatgg attgtgtggt
    10681 aaatggcatt aatgcccgca aactgatcct cgataactgt cagcatattc gtccgttcgt
    10741 acctgaactg gtggatggta aaccctggca gtcgtatgaa acagcgcaaa ttgcggttga
    10801 tctgcgcttc ttccagtttg taccagggga acactggcat tcttttgaag gctatgcaga
    10861 gaatcaatac tttgtcgatc cttgcaaact gttgctgaca accccaggta ttgatgcacg
    10921 taacggcgaa tatgaagcgt tcggtgtacc cgcgacgatt cttgctaact tcctgcgcga
    10981 aaatggcgta gtgccggaaa aatgcgatct taactccatc ctcttcctgc tgactccggc
    11041 agaagatatg gccaaacttc agcaacttgt gccctgctgg tacgcttcga aaaactgctt
    11101 gagtccgacg cgccattagc agaagtgctg ccttccatct acaaacagca tgaagagcgc
    11161 tacgccggtt ataccctgcg tcagttgtgc caggaaatgc atgatttgta tgcccgccac
    11221 aacgtgaaac aactgcaaaa agagatgttc cgcaaagcgc acttcccaca cgtcagcatg
    11281 aatccgcaag aagccaacta cgcctattta cgcggtgaag tggagctggt tcgtctgccg
    11341 gatgccgaag gtcgtatcgc tgccgaaggg gcgcttcctt atcctccggg tgtgctgtgt
    11401 gttgttccgg gtgaaatctg gggtggtgct gttttgcgtg ctctggaaga agggatcaac
    11461 ctgctgccag gttttgcacc ggagctgcag ggtgtctata tcgaagaaca tgatggtcgt
    11521 aagcaagttt ggtgctatgt catcaagcct cgtgatgcgc aaagcaccct gttgaaaggg
    11581 gaaaaattat gagtcaggct aaatcgaaca aaatgggcgt cgttcagtta accatactga
    11641 cgatggtcaa catgatgggc tccggtatca tcatgctgcc gacaaagctt gccgaagttg
    11701 ggacaatttc aattatctcc tggctggtga cagccgtagg ctcaatggca ctggcatggg
    11761 cattcgcaaa atgcggtatg ttcagccgta aatcaggtgg tatgggcggt tatgccgagt
    11821 atgctttcgg taaatccggt aactttattg cgaactatac ctacggcgtc tcactgctga
    11881 ttgctaacgt cgcgattgct atttcggcgg ttggttacgg caccgaactg ctcggcgcga
    11941 gtttgtcgcc agtgcagatt ggtcttgcga ccatcggggt gctgtggatt tgtaccgtgg
    12001 ctaactttgg tggtgcgcgc attaccgggc aaatcagtag cattaccgtg tgggggggca
    12061 ttattccggt ggtaggcctg tgcattatcg gctggttctg gtttagcccg acgctttacg
    12121 ttgattcctg gaatccgcat catgcaccgt tcttcagtgc agtaggttct tccatcgcta
    12181 tgacgctgtg ggcttttctt ggtctggagt ctgcgtgtgc gaatactgat gtagtggaaa
    12241 acccggaacg taatgtgcca atcgcggtac tcggcggtac gttaggtgcg gcggtgattt
    12301 atatcgtctc caccaacgtg attgcaggga ttgtgcctaa tatggagctg gcaaattcaa
    12361 cggcaccatt tggcctggcc ttcgcacaga tgtttacacc agaagtgggt aaagtcatta
    12421 tggcgctgat ggtgatgtcc tgctgcggtt cgctgcttgg ctggcagttc accattgccc
    12481 aggtgtttaa atcttcatct gatgaaggct acttccctaa aattttctcc cgtgtaacca
    12541 aagtggatgc accggtgcag ggaatgttga ccattgtgat tatccagagc gggttggcgc
    12601 tgatgacgat tagcccgtcg ctaaacagtc agttcaacgt gctggttaac ctggccgtgg
    12661 tgaccaatat cattccgtat attctgtcaa tggcggcatt agtcattatt cagaaggtcg
    12721 ctaatgtgcc cccctcaaaa gcgaaagttg caaactttgt tgcttttgtt ggcgcgatgt
    12781 atagctttta tgcgctgtac tcatccgggg aagaagccat gctgtacggt tccatcgtga
    12841 ccttccttgg ttggacactg tatggtctgg tatcaccacg ctttgaactg aaaaataaac
    12901 acggttaaaa aagcaaagta aaaaagggcg atcttgcgac cgctcttttt ttattaaatg
    12961 tgtttacgcg tttttcagaa cttcgctaac aatctcaacc gcttctttct caatctgctt
    13021 gcgatgttct tcaccgagga agctttcgca gtagatctta tatgcgtctt ccgtgcctga
    13081 cggacgcgcg gcgaaccagc cgttgtcagt catcactttc agaccgccaa tagaagcacc
    13141 gttgcccgga gcagcagtca ggcgcgcggt gatcgggtca cctgccaggg tgctggcgct
    13201 caccatttcc ggagacagct tagacagcgc cgctttttgt gcggaagtcg cagctgcctg
    13261 caaacggttg tagctcggcg caccaaagcg ttttgccagt tcgttgtagt gttcctgcgg
    13321 gttcttaccg gtgacagcgg tgatttccgc cgccagcaga cacataatga tgccgtcttt
    13381 gtcggtggac cacggcgtgc cgtcgaaacg caggaaggaa gcccctgcgc tctcttcgcc
    13441 gccaaagccg aagctgccgt cgaacagacc atcaacaaac catttgaaac ctaccggtac
    13501 ttctaccagc ttacggccca agtcattgac cacacggtcg atcatcgcag aagaaaccag
    13561 cgttttaccg acagcaacat ctttgcccca ctgcggacga tgctggaaca ggtagttgat
    13621 cgccaccgcc aggtagtggt tcggattcat caaacctgcc ggagtgacga taccgtgacg
    13681 gtcataatcc gggtcgttag caaacgccag atcgaactta tcacgcagtg ccagcagacc
    13741 cgccatcgca cactcggagg agcagtccat acggatcgca ccgtctttat cgaggtgcat
    13801 aaagcggaag gtttgatcga cctgatcgtt aacgatggtc aggttgaggt tgtaatactc
    13861 gccaatgcgc ttccagtatt cgataccgga accgcccaac ggatcaacgc ccagcgtcag
    13921 gcccgctttc tgaatcgccg ccatatcaac gatatcggcc agcccttcca cgaacggctg
    13981 caccagatcc tgctctttca catagccgga tgccatcgct tcgtcgaggg agatacgctt
    14041 cacgcctttc aggccatcgg ccatcagtgc gttggccctg tcttccacca ctttggtgac
    14101 gttagtatca gccgggccac catttggcgg attgtacttg ataccaccat cttccggcgg
    14161 gttatgggac ggtgtaatca cgataccgtc tgccagcggg ccaccttttt tattgtgaac
    14221 caggatggca ttggaaacgg caggcgtcgg agtgaagcca ttgttttcct gcacaatgac
    14281 atcaacgccg ttcgctgcca gcacttccag tacggaaatg aacgcaggct cggacagggc
    14341 gtgagtatct ttacccacat agcaagggcc agtgatgccg tttttcgcac gttcttcagc
    14401 aattgcctga gcgatcgcca gaatgtgcgg ctcgttaaag ctgtggcgcg ctgcactgcc
    14461 acggtgaccg gaagtaccga atttcaccgc gtgctccgca ttccctgctt ctggtttcag
    14521 tacataatat tgcgccgtca gttgggcgac gttaatcaaa tcactctgtt gtgcaggttg
    14581 gcctgcacga ttgtggattg ccattgcttt gtcctttgtc tgcaacgttt tagatagttc
    14641 cgcaaacctt ctcaatcaat tccgccggga attgcatcga ctgcatgatg tgttcgatca
    14701 tgctgcattt acggccggtg ttggtgttgg tgatcaccca atacggcgtg cctggcacat
    14761 gtttcggctt ggtctgatta ccatttttca gcagcgtttg ttcatctgcc gcaaagtaca
    14821 cgcgtgtacg accgtgcaac gattccgttg cttcggcaaa cgcctgggcg tcaagagaat
    14881 atagtgtaga caacagcagc ataaagcgat tgaccgctcg cttttgctct gcgtattcgt
    14941 ccgaaagcag aagttcacgc attgcgcgaa ccttgtcttt aatcgttttg accggcttcg
    15001 cttcgacgat agcaggtgac gcaacgcgaa cctctttcgt caccggagca gcaggctgtg
    15061 atgcggcgga aaatttcaac atacgccgta aaatgtcgga tgcgctctcg ccgatatgct
    15121 tagtgtggct ggcaatatag ctgtagagtt catcatcaac ttcaatcgtt ttcatcttaa
    15181 tccagtgcag tgtcttagct gaatacaagt cgccaagagt atatgaataa atcccggcag
    15241 cggatagcgt caagcctggc ttgcagaaaa actcggaata aaccttattt gttgcagccc
    15301 tgtggcacaa tggtcaacat aataccctaa cccgacaacg cgaaaaaaag aactttgcca
    15361 tgaaattaaa tatccgcgcg caaactgcac aaaaccagca caataattct cccatcgttc
    15421 ttgtccatgg tctgtttggt agcctcgaca accttggcgt actggctcgc gatctggtaa
    15481 acgatcacaa tatcatccag gttgatatgc gtaaccacgg tctttcaccg agagagccgg
    15541 taatgaatta cccggcgatg gcgcaggatc ttgttgatac cctggatgca ctgcagatcg
    15601 acaaagcaac atttatcggt cactccatgg gcggtaaagc ggtaatggca cttactgcac
    15661 tggcccccga tcgcatcgat aaactggtgg cgatcgatat cgcaccggtc gactatcacg
    15721 tacgccgtca tgatgagatt ttcgcagcta tcaacgcggt cagtgaatcg gacgcacaaa
    15781 ctcgccagca agcagcagca ataatgcgcc agcatcttaa tgaagaaggg gtgattcagt
    15841 ttctgttgaa atcttttgtt gacggggagt ggcgctttaa cgtgccggta ttgtgggatc
    15901 agtatccgca tattgtgggt tgggagaaaa tcccggcatg ggatcaccct gccctgttta
    15961 tccctggcgg caattctccg tatgttagcg agcagtaccg tgatgattta ctggcacaat
    16021 ttccacaggc acgggcgcat gtgattgcag gcgcgggtca ctgggtccat gctgaaaaac
    16081 cggatgcggt attacgcgct atccgtcgct atctcaatga ttaacgcaac tgattaaaaa
    16141 cccagcgtcc gcgtcgcttt ccgcgcgtgg gcgttggcgt gactcatcgc gctgatgtat
    16201 tatggcgcgc tatccatccg ggcagaggcc tggctgattg tttcccccga agtcaccaag
    16261 atcatggcca aagaacaaac ggaccgtacg acattagatc tgttcgcgca cgagcgtcga
    16321 ccgggacgac cgaaaactaa tccgctttcg cgcgatgaac agctgcgtat taataaacgc
    16381 aaccagctaa aacgcgacaa agtacgtggc cttaagcgtg tcgaactgaa gctgaacgcg
    16441 gaagctgtcg aggcgctgaa cgagctggcg gaatcgcgta atatgagccg tagcgaactg
    16501 atcgaagaga tgttaatgca gcaactggcg gcgctgcgta gtcagggcat cgtttaaatt
    16561 tccactttca tgtagcacag tgtgcagtcc tgctcgtttg ctgattccgc aagactgcct
    16621 gttctgctat gattgccttt atccgtgggc aattttccac ccccatttca ataagtttca
    16681 agaggttatt tcactcatgg ctatcactgg catctttttc ggcagcgaca ccggtaatac
    16741 cgaaaatatc gcaaaaatga ttcaaaaaca gcttggtaaa gacgttgccg atgtccatga
    16801 cattgcaaaa agcagcaaag aagatctgga agcttacgac attctgctgc tgggcatccc
    16861 aacctggtat tacggcgaag cgcagtgtga ctgggatgac ttcttcccga ctctcgaaga
    16921 gattgatttc aatggtaaac tggttgcgct gtttggttgt ggtgaccagg aagattacgc
    16981 cgaatatttc tgcgacgcat tgggcaccat ccgcgacatc attgaaccgc gcggtgcaac
    17041 catcgttggt cactggccaa ctgcgggcta tcatttcgaa gcatcaaaag gtctggcaga
    17101 tgacgaccac tttgtcggtc tggctatcga cgaagaccgt cagccggaac tgaccgctga
    17161 acgtgtagaa aaatgggtta aacagatttc tgaagagttg catctcgacg aaattcttaa
    17221 tgcctgatgt gatgcggcgt agactcatgt ctacgccgta ttaatagata atgccaatca
    17281 aaataattgc tacaaatttg taacttttgc tgttgtacct gtacaatgtc ccagtgttca
    17341 agtggccttg ccgttgtaaa tgtaagctgt gccacgtttt tattaacaat atttgccagg
    17401 gacttgtggt tttcatttag gcgtggcaat tctataatga tacgcattat ctcaagagca
    17461 aattctgtca cttcttctaa tgaagtgaac cgcttagtaa caggacagat tccgcatgac
    17521 tgataacaat accgccctaa agaaagctgg cctgaaagta acgcttcctc gtttaaaaat
    17581 cctggaagtt cttcaggagc cggacaacca tcacgtcagt gcggaagatt tatacaaacg
    17641 tctgatcgat atgggtgaag aaattggtct ggctacggta tatcgcgtac tgaaccagtt
    17701 tgacgacgct ggtatcgtca cccgccacaa ttttgaaggc ggtaaatccg tatttgaact
    17761 gacacagcaa catcaccacg atcacctgat ttgcctcgac tgcggcaagg ttatcgaatt
    17821 tagtgatgat tccatcgaag cgcgtcagcg tgaaattgcc gcaaaacatg gcattcgcct
    17881 gactaaccac agtctctatc tttacggtca ctgtgccgaa ggcgattgcc gcgaagatga
    17941 gcacgcgcac gaaggcaaat aagccagcct gaacgagaaa agccaacctg cgggttggct
    18001 tttttataca agggaaataa gaaaagcgag agttacagct ctcacttatt tgttattact
    18061 gcgtatttcc tgccagacct tatcacaatc ggctttcacc gcctgatcat ttccggtttg
    18121 cgttgcacgc agacactgct gataatccag tacgcgtaca ctttcctggt cggcaaactg
    18181 ctgatgttgt ttctctttct tcagcacgtt taacacgctc tggcaggctt caattttttc
    18241 tggcgaacct tgcgcggtgt tgatacaggc gctgtatgcc tctttcagac ggctgtcttc
    18301 ctgtggtgcg gttgattgcg cacaagctac cagccccgat gccatcatgg cgatgagaat
    18361 cagttttttc attgaatgcg gcaggcacaa aacctgccgc gtcgggcatc agaagatggt
    18421 gaatggtgcg attaccataa atttcacgtc acgctcatcc tggaagatgt taccgtaacc
    18481 gccgccccag cttgggatgt cggagtggtt gtcgtattcg gtgaagtgca gtttgaacat
    18541 cgtgcctttg gcgcgaccgt cctgaatggt gtagaccgca tccaggctgt aggcagactc
    18601 ttcaatagtc cggtttttgt cgtagtacgc atccggattg ctctgccagg tcgcaggttt
    18661 agcatcccat gcgtaaacgt aggaagcgcc gatggcgaag cctggaagat tccagttttt
    18721 caggtcatac atcgcactga agaagaccgc tttttcgccg ttagcgttga agtcggaacg
    18781 gttatcccac cagatatcca ggcggccgtt ggaggaagcg taggttgggg tcatacgttg
    18841 caggaagtat ccctgctgac tgtcagcctt aatccaggtg ccttcgaggc gcaaatccac
    18901 tacgtcagcc gcccggtaac caaaggtcaa cgcttgcagc caggcggtgc cgtcataaag
    18961 atcgttgacg ctgcgatcgt caactttatc gcgggtacca tagaactggt agctggtggt
    19021 taacgggcta ccggcgatat caaatttgta gctggctttg gcaaaatatt gatcgatata
    19081 cccttccgcc tgaccaaatg ccgcttccag tacgaagtta tttttgaagt cgtatttcgc
    19141 accgatggag tgcagataat caactttggt ggttttatcg ttctgataaa actcatccat
    19201 ttccagatgc cacggcgctt tgtattcgtt ggtccacatg taggagaaac tcaaggcacc
    19261 agcatcgccg taatcaaaat tcgccccggc ttccgccccc tgataagtac ccggcataaa
    19321 gctccagtgc ggcgctaaca gcgtttgacc ggttggctga atgtaacctg ccctcgccca
    19381 aaccggaccg tatttaaatt tggccgcagc tttatacagg cttataccgc ttttgtcgcc
    19441 ggaccagtct tcgtcatagg ctttattact ttttgaaaac gcgatttcgt tcgggtggga
    19501 gctgtcgccg ttttccgcca tttcaatcgc cgtaaacgca gcaatatcaa ggccgaacat
    19561 atcagcagca taaccggact gaaaatcgag gttggcattc caggtggagt gagaaaggtt
    19621 ggttttgtat ttgtcgccgt cggtaacatc tttacggtcg cgttcacgct gccagtaata
    19681 gataccgccg gttaaggttg aatcgtcgat gaatccttcg gcgcgagcct ccggcattgc
    19741 cataaagccc gacattgctg taacaccggc aatagccagc gccagcgtac tacgtttgcc
    19801 actaaacgta cgcatgggtt aatcctcttt gacgtataaa ttgctgcacc aaaggtgaac
    19861 agcgaaaaaa aataaaaatt aaaaaagtag cgttgagaaa agtctcgctg actacaggaa
    19921 cagactattt ttcgcgacgc gaattatcaa tctctttctg taaccagaat gtaagaacat
    19981 gagcaaccgc acatattttg atgatttttg ttacaaggct taagtttgcg gttgaaaggc
    20041 ttgcagaaga ataaaaacgt gaaattccag caagatgaaa gcggttacat tttatttaat
    20101 tatgttttgt gattttaaat ttattttatg gataaattgc gaagcggaat taattcgcga
    20161 agaaaagcag aaaaaacgcc gcagagcgcg gcgtttggga ttgtttgcta tttcttgccg
    20221 gatgcggcgt gaacgcctta tccggcctac aaaagcacgc aaattcaata tattgcagag
    20281 atcatgtagg cctgataagc gaagcgcatc aggcaattta gcgtttgaaa tttattgtct
    20341 atttattgcc ggatgcgacg taaatgcctc acccggcttg gtagaactta aaattactcg
    20401 cctactttcg cccaggtatc acgcagccca acggtgcggt taaataccgg tttttcggcc
    20461 gtggaatggc ggctgtcgag gcagaagtaa ccttcacgct caaactggaa tgctttaccc
    20521 gcaaccgcat ctttcagcga cggttcagca aagccctgtt tgatcaccag cgattccggg
    20581 ttaatcaccg acaggaaatc atccgcagca cctgggttag gcacgctgaa cagacgatca
    20641 tacaaacgga tttcaaccgg cagcgcatgt gccgcgctca cccagtgaat aacacctttg
    20701 actttacgac catctgccgg atctttgctt aaggtgtcgg cgtcataagt acagaagatg
    20761 gtggtgatat taccttcggc atctttctcc acgcgttcag ccttaatgac gtaagcatta
    20821 cgcagacgca cttctttacc cagcaccaga cgtttgtact gcttgttagc ttcttcgcgg
    20881 aaatcggcgc gatcgatcca aatttcaccg ctaaacggca cctgacggct ccccatttcc
    20941 ggtttgttcg gatggttcgg catggtgacc atttcgcctt cgccctgata gttttcgata
    21001 accagtttca ccggatcgat aaccgccatt gcgcgcggcg cattttcgtt gagatcttca
    21061 cggatgcagg attccagcga cgccatctca atggtgttgt cctgcttggt cacgccgatg
    21121 cgtttgcaga actcacgaat agaagccgca gtgtaaccac gacgacgcag accggaaatg
    21181 gtcggcatac gcgggtcatc ccagccttca acgtgcttgt cggtcaccag caggttcagc
    21241 ttacgcttgg acatcacagt gtattccaga ttcaggcgcg agaattcgta ctggcgcgga
    21301 tgaacaggaa tggtgatgtt atccagtacc cagtcgtaca gacgacggtt gtcctggaac
    21361 tcaagcgtac acagagagtg cgtaatacct tccagcgcat cgctgatgca atgggtgaag
    21421 tcgtacatcg ggtagatgca ccacttgttg ccagtctggt ggtgttcagc aaatttaatg
    21481 cggtacagca ccggatcgcg cattacgata aatggcgacg ccatgtcgat tttcgcacgc
    21541 aggcaggctt taccttcttc aaaaccaccg gcacgcattt tttcgaacag cgccaagttc
    21601 tcttcaacgc tgcggtcgcg gtacgggctg tttttacccg gctgcgtcag ggtaccgcgg
    21661 tattcgcgga tctgttccgg tgtcagttca tcaacgtacg ccaggccttt attgatcagt
    21721 tcgatcgcat aggcgtggag ctgatcaaaa taatcggagg agtaacggac gttaccagac
    21781 cagtgaaaac ctaaccactc tacgtcgttt ttgatcgaat cgacatactc gatatcttct
    21841 tttaccgggt tagtgtcgtc gaaacgcagg ttgcactggc ctttatagtc ctgggcgatc
    21901 ccgaagttca ggcagataga tttcgcatgg ccaatatgca gatagccatt cggctccggc
    21961 gggaaacggg tgtgtactgt gatgtgctta ccactggcca gatcttcatc gatgatctga
    22021 cggataaagt tagtcgggcg ggcttctgcc tcactcatcg tggattcctc aaagcgtaaa
    22081 caacgtataa cggcgtatga tcttataagc gggacaggct gacaactgtt agttttttaa
    22141 aattgataat ctaacaagga ttatggggat gattgatgaa aaaaaaagcg gcagagaaga
    22201 tctccaccgc ttgtcattgt tggatgcgac gctgccgtgt cgcatcaggc ataaagcaga
    22261 ttactttttg atttcataca gcggtgtttg acccgccaca acatggccct gagctttaat
    22321 gagcaagcca ctgaaatcgt cgatattgct gcaaaccacc gggctaatca tcgagcgggc
    22381 gttagcgttc aggtaatcca gatccatttc cagaatcggt tgccctgcgc ttacctgcgc
    22441 accctcttcc accagacgtt taaagccttt accttccagc gctacggtgt cgatacccat
    22501 atggacgacg atctccgcgc ctttttcggt ttccaggcag aacgcgtggt tggtgttgaa
    22561 gattttcacg attgtccctg cggctggtga tacgacgatt ttatctgtcg gtttcaccgc
    22621 cacaccgtca cccaccgctt tgctggcgaa tgcttcgtca ggaacctgat ccagtgccac
    22681 gacatcaccg gtaatcggcg ataccagctc cgcgatagat accgcgtttg gtacagcctg
    22741 cggttttgct acaggcgcgg cagttgccgg agttgcttca gctgacgcag cggctaccgg
    22801 accacgggca acgactttct tcatcgcatc gccgatggat tctgctttcg cgccaacaat
    22861 cacctgaata gtctgtttgt tcagtttcac tacaccagaa gcacccagac gtttacacat
    22921 cgtatcgttg acgcgggcag agtcagccac ggtcaggcgc agacgggtaa tacaggcatc
    22981 aatcgctttc aggttgtcag taccgccaac cgcagcaata tagttggttg ccagttgagt
    23041 cagaccttct tcagtattgc tgttggcttc ttcagtaacg atctcgtctt ctttatcttc
    23101 acgacccggc gttttcaggt tgaacatgcg gataaccaaa ctgaacacca cgaagtagat
    23161 agcgaagaag ataacgccca tcaccagcag catccagacg ttctggctgg cggcaggcag
    23221 gttatacatc aacgcgtagt cgatagcacc cgcagagaaa gagaagccgg catggatacc
    23281 cagcagcgtt gccacaaaca ggctgatacc ggtcagcagt gcgtgcagga ggtacagcag
    23341 cggcgcaagg aacatgaaca ggaattccag cggctcagtc acaccggtca ggaacgcagt
    23401 aacagcaaca gaaagcagca tcccgccaac catcggacga cgctctttcg gtgctgcgaa
    23461 gtacatcgcc agcgccgcac ccggcagacc gaacatcatg atcgggaaga agccggacat
    23521 gaacatcccc gcggtgccgt caccagcgta gaaacggttg atgtcgccgt ggaaaaccgt
    23581 acccgccgcg ttggtgaatt caccaatctg gaaccaggcg atggtgttca gcacctgatg
    23641 cagaccggtt gggatcagca gacggttgat gaaaccaaag ataccggaac ccagcgcgcc
    23701 cgcagaaacg atccactcgc cgcctgcatg gatagcgtgc tgtaccggcg gccagacgta
    23761 accaaaaatg gccgccagca ccagacagaa gaagccggtg gcgatcggca caaagcgttt
    23821 gccgccgaag aagctcagga agtccggcag tttaatatcg gaccaacggt tataggctgc
    23881 gccaccaacc agaccggtaa tgatacccgc cagtacaccc atgttaattt ctgggttgat
    23941 ggtcaccatc gctttggtta acacaaagta acctaccgca cccgccagcg ccgccgcacc
    24001 tgcgctgtct ttcgaccagc tggatgccac accgatggcg aagattaatg cgaggttatc
    24061 aaaaattgca ccgcccgcct gggcaataaa cgcaacgtta agtaaatctg gctgaccgaa
    24121 tcgcagcaac agcgccgcca ccggcagcac cgcgataggg agctgtaacg ccctaccgag
    24181 tcgctggaaa aaacctaaaa tattcatctt attcccccta cgagaaccct atttggctcg
    24241 tttaaagccg tatttttatt ttgctgcaaa ttttactgcc gatgttctgt aatcagattg
    24301 ttagatcatc tgctacagag tgtgtgaaaa tttaattcgc atcgcaaatt aaacgcgtgt
    24361 cttttgtgag ttttgtcacc aaatatcgtt atcatcactc ccttttactg gctaaaccag
    24421 aaaacttatt ttatcattca aaaaatcaag tcggattgac gcctgtctgc gcaaatccag
    24481 gttacgctta aagatgtcta atccgccaac ggcttacatt ttacttattg aggtgaataa
    24541 tgagactgat ccccctgact accgctgaac aggtcggcaa atgggctgct cgccatatcg
    24601 tcaatcgtat caatgcgttc aaaccaactg ccgatcgtct gtttgtactg ggcctgccga
    24661 ctggcggcac gccgatgacc acctataaag cgttagtcga aatgcataaa gcaggccagg
    24721 tcagctttaa gcacgttgtc accttcaaca tggacgaata tgtcggtctg ccgaaagagc
    24781 atccggaaag ctactacagc tttatgcacc gtaatttctt cgatcacgtt gatattccag
    24841 cagaaaacat caaccttctc aacggcaatg ccccggatat cgatgccgag tgccgccagt
    24901 atgaagaaaa aatccgttct tacggaaaaa ttcatctgtt tatgggcggt gtaggtaacg
    24961 acggtcatat tgcatttaac gaaccggcgt cttctctggc ttctcgtact cgtatcaaaa
    25021 ccctgactca tgacactcgc gtcgcgaact ctcgtttctt tgataacgat gtcaatcagg
    25081 tgccaaaata tgccctgact gtcggtgttg gtacactgct ggatgccgaa gaagtgatga
    25141 ttatggtgct gggtagccag aaagcactgg cgctgcaggc cgccgttgaa ggttgcgtga
    25201 accatatgtg gaccatcagc tgtctgcaac tgcatccgaa agcgatcatg gtgtgcgatg
    25261 aaccttccac catggagctg aaagttaaga ctttaagata tttcaatgaa ttagaagcag
    25321 aaaatatcaa aggtctgtaa ttgttatccc tgccctggtt ccttgctcag ggcaatattt
    25381 tttaaaatcg ggggtcagaa tgtatgcatt aacccagggc cggatcttta ccggccacga
    25441 atttcttgat gaccacgcgg ttgttatcgc tgatggcctg attaaaagcg tctgtccggt
    25501 agcggaactg ccgccagaga tcgaacaacg ttcactgaac ggggccattc tctcccccgg
    25561 ttttatcgat gtgcagttaa acggctgcgg cggcgtacag tttaacgaca ccgctgaagc
    25621 ggtcagcgtg gaaacgctgg aaatcatgca gaaagccaat gagaaatcag gctgtactaa
    25681 ctatctgccg acgcttatca ccaccagcga tgagctgatg aaacagggcg tgcgcgttat
    25741 gcgtgagtac ctggcaaaac atccgaatca ggcgttaggt ctgcatctgg aaggtccgtg
    25801 gctgaatctg gtaaaaaaag gcacccataa tccgaatttt gtgcgtaagc ctgatgccgc
    25861 gctggtcgat ttcctgtgtg aaaacgccga cgtcattacc aaagtgaccc tggcaccgga
    25921 aatggttcct gcggaagtca tcagcaaact ggcaaatgcc gggattgtgg tttctgccgg
    25981 tcactccaac gcgacgttga aagaagcaaa agccggtttc cgcgcgggga ttacctttgc
    26041 cacccatctg tacaacgcga tgccgtatat taccggtcgt gaaccgggcc tggcgggcgc
    26101 gatcctcgac gaagctgaca tttattgcgg tattatcgct gatggcctgc atgttgatta
    26161 cgccaacatt cgtaacgcta aacgccttaa aggcgacaaa ctgtgtctgg ttaccgacgc
    26221 caccgcgcca gcaggtgcca acattgaaca gttcattttt gcgggtaaaa caatatacta
    26281 ccgtaacgga ctttgtgtgg atgagaacgg tacgttaagc ggttcatcct taaccatgat
    26341 tgaaggcgtg cgtaatctgg tcgaacattg cggtatcgca ctggatgaag tgctgcgtat
    26401 ggcgacgctc tatccggcgc gtgcgattga cgttgaaaaa cgtctcggca cgctcgccgc
    26461 aggtaaagta gccaacctga ccgcattcac acctgatttt aaaatcacca agaccatcgt
    26521 taacggtaac gaggtcgtaa ctcaataaga gaaagtatga caccaggcgg acaagctcag
    26581 ataggtaatg ttgatctcgt aaaacagctt aacagcgcgg cggtttatcg cctgattgac
    26641 cagtacgggc caatctcgcg gattcagatt gccgagcaaa gccagcttgc ccccgccagc
    26701 gtaaccaaaa ttacgcgtca gcttatcgaa cgcgggctga tcaaagaagt tgatcagcag
    26761 gcctccaccg ggggccgccg cgctatctcc atcgtcaccg aaacccgcaa tttccacgca
    26821 atcggcgtac ggcttggtcg tcatgacgcc accatcactc tgtttgatct cagcagcaaa
    26881 gtgctggcag aagaacatta cccgctgccg gaacgtaccc agcagacgct ggaacatgcc
    26941 ctgctgaatg ccattgctca gtttattgat agctaccagc gcaagctgcg cgagctaatc
    27001 gccatttcgg tgatcctgcc agggcttgtt gacccggaca gcggcaaaat tcattacatg
    27061 ccgcatattc aggtagaaaa ctgggggctg gtagaagcac tggaagaacg ttttaaagtg
    27121 acctgtttcg ttggtcacga tatccgtagt ctggcgctgg cggagcacta cttcggtgca
    27181 agtcaggatt gcgaagactc cattctggtg cgtgtccatc gcggaaccgg ggccgggatt
    27241 atctctaacg ggcgcatttt tattggccgc aacggtaacg tcggtgaaat tggccatatt
    27301 caggtcgaac cgctgggtga acgctgccac tgcggcaact ttggctgcct ggaaactatc
    27361 gccgccaacg ctgccattga acaacgggtg ttgaatctgt taaagcaggg ctaccagagc
    27421 cgtgtgccac tggacgactg caccatcaaa actatctgca aagccgcgaa caaaggcgat
    27481 agcctggcct cggaagtgat tgagtatgtc ggtcgtcatc tgggtaaaac catcgccatt
    27541 gctatcaact tatttaatcc gcaaaaaatt gttattgctg gtgaaatcac cgaagccgat
    27601 aaagtgctgc tccctgctat tgaaagctgc attaataccc aagcgctgaa ggcgtttcgt
    27661 actaatctgc cggtggtacg ttctgagctg gatcaccgct cggcaatcgg cgcttttgcg
    27721 ctggtaaaac gcgccatgct caacggtatt ttgctccagc atttgctgga aaattaatgt
    27781 gcttttatag tggcgcttat tgttgtcaat attctgggta gtccatgacc attaaaaatg
    27841 taatttgcga tatcgacggc gtgctgatgc acgataacgt cgccgtaccg ggtgcagcgg
    27901 aatttttgca cgggattatg gataaaggcc tgccgctggt gttgctgacc aactatcctt
    27961 cgcagactgg gcaagatctg gcgaaccgct ttgccactgc cggtgtcgat gtgccggata
    28021 gcgtgtttta tacctctgcg atggcgaccg ccgatttcct gcgtcgccag gaaggcaaga
    28081 aagcgtatgt ggtgggcgaa ggcgcactga ttcatgaact gtacaaagcc ggtttcacta
    28141 ttaccgatgt gaaccctgat ttcgtgattg ttggcgaaac acgttcctac aactgggaca
    28201 tgatgcataa agcagcctat ttcgtcgcta acggtgcacg ctttatcgcc accaacccgg
    28261 acacccacgg gcgcggtttt tatcccgctt gtggcgcgtt gtgtgcaggg attgagaaaa
    28321 tctccgggcg caaaccgttc tatgttggta agcccagccc gtggatcatc cgcgcggcat
    28381 taaacaaaat gcaggcgcat tcggaagaaa cagtgattgt cggcgataac ctgcgtaccg
    28441 atattctggc gggcttccag gccggtctgg agacgattct ggtgctttct ggtgtttcat
    28501 cgctcgacga tatcgacagt atgccgttcc gcccaagctg gatttacccg tcagtcgctg
    28561 aaatcgacgt tatctgaata ccacccacgt ctgcggacgt ggttggctct gatgacaaac
    28621 gccaaactgc ctgatgcgct acgcttatca ggcctacgca gctcctgcaa tatattgaat
    28681 tttcatgctt ttgtaggccg gataaggcgt tcacgccgca tccggcatga acaaatcgca
    28741 ctttatcagc aatttggccc ccgtaaaata taaccattca ataaaacccg ctaaaaatta
    28801 tcgattcatc aataaataca tcctcacacc agaccctttt tcaccaatcg acaatcacca
    28861 ttacgttttt tatttttgca ctgccaaata gcttgcgcac agtgctgctt tgcggcattt
    28921 ttttaaacaa gcaaacacaa caagcaacaa ataccaggtt aacggagaag gttatgtgtt
    28981 caatttttgg cgtattcgat atcaaaacag acgcagttga gctgcgtaag aaagcactcg
    29041 agctgtcacg cctgatgcgt catcgtggcc cggactggtc cggtatttat gccagcgata
    29101 acgccattct cgctcacgaa cgtctgtcaa ttgttgacgt taacgcgggg gcgcaacctc
    29161 tctacaacca acaaaaaaac cacgtactgg cggtaaacgg tgaaatctac aaccaccagg
    29221 cattgcgcgc tgaatatggc gatcgttatc agttccagac agggtctgac tgtgaagtga
    29281 tcctcgcgct gtatcaggaa aaagggccgg aatttctcga cgacttgcag ggcatgtttg
    29341 cctttgccct gtacgacagc gaaaaagatg cctacctgat tggtcgcgac catctgggga
    29401 tcatcccact gtatatgggg tatgacgaac acggtcagct gtatgtggcc tcagaaatga
    29461 aatcgctggt gccagtttgc cgcacgatta aagagttccc ggcggggagc tatttgtgga
    29521 gtcaggacgg cgaaatccgt tcttattatc atcgcgactg gttcgacttt gatgcagtga
    29581 aagataacgt gaccgacaaa aacgagctgc gtcaggcact ggaagattcc gttaaaagcc
    29641 atctgatgtc tgatgtgcct tacggtgtgc tactttctgg tggtctggat tcctcaatta
    29701 tttccgctat caccaagaaa tacgcagccc gtcgcgtgga agatcaggaa cgctctgaag
    29761 cctggtggcc gcagttacac tcctttgctg taggtctgcc gggttcaccg gatcttaaag
    29821 cagcccagga agtggcaaac catctgggca cggtgcatca cgaaattcac ttcactgtac
    29881 aggaaggtct ggatgccatc cgcgacgtga tttaccacat cgaaacttat gatgtgacca
    29941 ctattcgcgc ttcaacaccg atgtatttaa tgtcgcgtaa gatcaaggcg atgggcatta
    30001 aaatggtgct gtccggtgaa ggttctgatg aagtgtttgg cggttatctt tacttccata
    30061 aagcgcccaa cgctaaagaa ctgcatgaag agacggtgcg taaactgctg gccttgcata
    30121 tgtatgactg cgcgcgcgcc aacaaagcga tgtcagcctg gggcgtggaa gcacgcgttc
    30181 cgttcctcga caaaaaattc cttgatgtgg cgatgcgcat taacccgcag gataaaatgt
    30241 gcggtaacgg caaaatggaa aaacacatcc tgcgtgaatg ttttgagtca tacctgcccg
    30301 caagcgtggc ctggcggcag aaagagcagt tctccgatgg cgtcggttac agttggatcg
    30361 acaccctgaa agaagtggcg gcgcagcagg tttctgatca gcaactggaa actgcccgct
    30421 tccgcttccc gtacaacacg ccgacctcaa aagaagcgta tctgtaccgc gagatctttg
    30481 aagaactgtt cccacttccg agtgccgctg agtgcgtgcc gggcggtcct tccgtcgcgt
    30541 gttcttcagc taaagcgatc gaatgggatg aagcgttcaa aaaaatggac gatccgtctg
    30601 gtcgtgcggt tggcgttcac cagtcggcgt ataagtaaga aatgcggtac tgccccggag
    30661 aaatccgggg ctgttttagt tattgcgctg cttgttgatt ctgatatgca gtaaggaaat
    30721 gttccgggtt tgccacatca ctgtttgaca actcatcact gtaggccagt atttcataga
    30781 ttaatttccc accactattg ttgaattccg aatttcatcc tgtacgacaa aagaatggct
    30841 gtagggtggg gaaaaacaca aagtaaacaa taattgacga atatagcgcc acgctgttcg
    30901 caacctaacc aaacagtcac tttcgagcaa ttttccttga aaaagaggtt gacgctgcaa
    30961 ggctctatac gcataatgcg ccccgcaacg ccgataaggt atcgcgaaaa aaagatggct
    31021 acgtagctca gttggttaga gcacatcact cataatgatg gggtcacagg ttcgaatccc
    31081 gtcgtagcca ccatcttttt tgcgggagtg gcgaaattgg tagacgcacc agatttaggt
    31141 tctggcgccg caaggtgtgc gagttcaagt ctcgcctccc gcaccattca ccagaaagcg
    31201 ttgtacggat ggggtatcgc caagcggtaa ggcaccggtt tttgataccg gcattccctg
    31261 gttcgaatcc aggtacccca gccatcttct tcgagtaagc ggttcaccgc ccggttattg
    31321 gggtatcgcc aagcggtaag gcaccggttt ttgataccgg cattccctgg ttcgaatcca
    31381 ggtaccccag ccatcgaaga aataatctgg ctacgtagct cagttggtta gagcacatca
    31441 ctcataatga tggggtcaca ggttcgaatc ccgtcgtagc caccaaattc tgaatgtgtc
    31501 gaatacgttc ggcaaattca aaaaccaatt tgttggggta tcgccaagcg gtaaggcacc
    31561 ggattctgat tccggcattc cgaggttcga atcctcgtac cccagccaat ttattcaaga
    31621 cgcttacatc gtaagtgcac ccagttgggg tatcgccaag cggtaaggca ccggattctg
    31681 attccggcat tccgaggttc gaatcctcgt accccagcca cattaaaaaa gctcgcttcg
    31741 gcgagctttt tgcttttctg cgttcattca atgtcgaatg cgatgttgac acgtcttatc
    31801 cttcaatgtc ggatgcgacg ctgccgcgtc ttatccgacc tacggctggc acgcatccgg
    31861 caatgttgta aggctacaac cctaacgcat atttcagcgc ctgacgtttc aacacgccag
    31921 cacgctctgc cgccattaac ccgagattac gcacaaaacg cagcggcggc agattattac
    31981 tgaatccggc ataaaacaga tccataccac tttgcataat gaagttatcc gccatgcgcc
    32041 gcatctggta acgcttgagg acaggataac tggcccacgc ttcaccgtag ctgcgggcat
    32101 tcaccagaac atcaatcagg gcatcgacat cacgataacc aagattcacc ccctgccccg
    32161 ccagcggatg gatggtatgc gcggcatcgc ccaccagcgc aagccccggc tgcacatact
    32221 gcaacgcatg tcggcgcgtc agcggaaacg caccagcggc aagcggtgta acgtaaccca
    32281 gacgcgacgg gaaatgcttc gcgatttccg cctggagttg cgccatattc atattctgca
    32341 actggcgaat acgcgccgga gagtcatacc acaccagcga tgcccagtta tcaaacaacg
    32401 gcagaaacgc acgcggtccg tccggggtaa attgctgcca ggtgctgtcg ccgggatcgt
    32461 tctcacactg tacgctaatc aacatgcacg actgcgcata ctgccaggca tgaacgccaa
    32521 ttcccgccat ctgccgcacc tgagaatttg cgccgtcggc accaatcacc agtttcgcgc
    32581 ggatcgtttc accgcccttc agttccagct cctgaagatc attatctcga tgtaatgcaa
    32641 tcagcgaggt tggcacacgt aacgttactt tcggatgcgc ttccagcgcc tgccacaacg
    32701 cctgttgcag gactgtgttt tccaccatgt agccaagcaa cggcaacttt aattcggctg
    32761 cgtcaaatac cacatgtgcc gtttcccact cccaggtttc cagtctgcgg taaggatggc
    32821 aacgcatagc ctgtactgca tcccagaccc ctaacccttt aagcaatgat accgaagccg
    32881 cgctgatcgc cgagatccgc acgtccggtt ggctgtcagc gacaaacggt gcaggttctg
    32941 cgtgctcgat caccgttacc gaaaatccgt gctgtgccag ccccagcgcc agtgcgccgc
    33001 cgaccattcc tccgccgaca atggcaattt ccgttggttg atttgtcatg gtcaatcatc
    33061 ctgttagcaa tatgctttaa gtttacagga tttttacccc caagggctga taacgctcat
    33121 actggtcaca acggcagcaa agcattacac tatgcgccct gcattcctgg ctactatttc
    33181 gcaagagcaa gtcgatgacc aaaaaactcc atattaaaac ctggggctgt cagatgaacg
    33241 agtacgattc atcgaagatg gccgatctgc tggatgccac ccacggctat caactgaccg
    33301 acgtggcgga agaagcggat gtgctactgc tgaacacctg ctcaatccgc gagaaggctc
    33361 aggaaaaagt cttccatcag ttgggtcgct ggaaactgtt aaaagagaag aatccagacc
    33421 tgattatcgg cgtcggtggc tgcgtggcat cgcaagaagg cgagcacatt cgccagcgcg
    33481 cccactatgt cgatattatt tttgggccgc aaacgctgca ccgtctgccg gagatgatca
    33541 actccgtgcg cggcgaccgc agcccggttg tagatatcag cttcccggaa atcgagaagt
    33601 ttgaccgtct gccggaaccg cgcgccgaag ggccgaccgc gtttgtctcc atcatggaag
    33661 gctgcaataa atattgcacc tactgcgtgg tgccttacac ccgtggtgaa gaggtaagcc
    33721 gtccgtctga cgatattctg tttgagattg cccagcttgc ggctcagggc gtgcgtgaag
    33781 tcaacctgct cggtcagaat gtgaatgcct ggcgtggtga gaactacgac ggcaccaccg
    33841 gatcgtttgc cgatctgttg cgtctggttg ctgcgatcga cgggatcgat cgtattcgct
    33901 ttaccaccag ccatccgatc gaattcaccg acgatatcat cgaagtgtac cgcgacacgc
    33961 ctgagctggt gagcttcctg catctgccgg tacagagcgg ttccgatcgc attctgaacc
    34021 tgatgggacg tacccatacg gcgctggagt acaaagcgat catccgtaaa ctgcgtgcgg
    34081 cgcgtccgga tattcagatc agttctgact tcattgttgg cttccctggc gaaaccaccg
    34141 aagacttcga gaaaacgatg aagctgattg ccgacgtcaa tttcgacatg agctacagct
    34201 ttatcttctc tgcacgtcca ggaacgcctg ccgccgatat ggttgatgat gttccggaag
    34261 aagagaagaa gcagcgtctg tatattctgc aagaacgtat caatcagcaa gcgatggcat
    34321 ggagccgtcg gatgctcggc accacccagc gtattctggt agaagggact tcgcgtaaga
    34381 gcatcatgga gctttccggg cgtaccgaaa ataaccgcgt ggtcaacttc gagggcacac
    34441 cggatatgat cggtaaattc gtcgatgtag aaattaccga cgtctacccg aactctctgc
    34501 gcggtaaagt ggtgcgaact gaagatgaaa tgggtctgcg catggcagaa acaccggagt
    34561 cggtgattgc ccgcacccgc aaagaaaacg accttggcgt gggttattat cagccgtaat
    34621 tctcaggcct gccgttccgg tgggccttgt tttttctctc tttatcccca tctttatcgc
    34681 aatgcttgcg cctttttcct gtggcgtgaa taatttcctt ataggccggt gaatgttcag
    34741 cttccggcgg catacagata agaggaacgg tttgaacata gacactcgcg aaatcaccct
    34801 ggagccagca gacaatgcgc gtctgttgag cctgtgcggc ccgtttgatg acaacatcaa
    34861 gcagctcgaa cgccgtctcg gcatcgagat caatcgccgc gataaccact ttaaactgac
    34921 cggccgtccg atttgcgtca ccgctgcggc agacattctg cgtagcctgt atgtcgatac
    34981 tgccccgatg cgcggtcaga ttcaggatat cgaaccggaa cagatccacc ttgcgattaa
    35041 agaagcgcgg gtactggagc aaagcgcgga gagcgtgccg gagtacggca aagcggtcaa
    35101 tatcaaaact aaacgcggcg taattaagcc gcgcacgcca aaccaggcgc agtacatcgc
    35161 caatattctc gaccatgaca ttaccttcgg cgttggcccg gcgggtacgg gtaaaaccta
    35221 cctggcagtg gctgcggcag ttgatgccct ggagcgtcag gaaattcgcc gtattctgct
    35281 gactcgtccg gcggtcgaag ccggtgagaa actgggcttc ctgcctggcg atttaagcca
    35341 gaaagtagac ccgtatctgc gcccactgta cgacgcgctg tttgaaatgc tgggctttga
    35401 gaaagtcgag aaactgattg agcgcaacgt tattgaagtc gcgccgctgg cctatatgcg
    35461 tggtcgtacg ctgaacgacg cgtttatcat tctcgatgag agccagaaca ctaccatcga
    35521 acagatgaag atgttcctga cccgtatcgg ttttaactca aaagcggtta tcaccggcga
    35581 cgtcacacag atcgacctgc cgcgtaatac taaatcaggc ttacgtcatg ccattgaagt
    35641 gctggccgat gtcgaagaga tcagctttaa cttcttccac agcgaagacg tggttcgtca
    35701 cccggtggtg gcgcgtatcg ttaacgctta tgaagcctgg gaagaagcag aacaaaaacg
    35761 taaagcggcg ctggcagcag aacgcaagcg cgaagaacag gaacaaaaat gagtcaggtg
    35821 atcctcgatt tacaactggc atgtgaagat aattccgggt taccggaaga gagccagttt
    35881 caaacatggc tgaatgcggt gatcccgcag tttcaggaag agtcggaagt gactattcgc
    35941 gtggtcgata ccgccgaaag ccacagtctg aatctgacct atcgcggtaa ggataagccg
    36001 accaacgtgc tctccttccc gtttgaagtg ccgcctggca tggagatgtc gctactgggc
    36061 gatctggtta tctgccgtca ggtggttgag aaggaagctc aggagcaagg caaaccactg
    36121 gaggcgcatt gggcgcatat ggtggtgcac ggcagtctgc atttgttagg ttacgatcac
    36181 attgaagatg acgaagcaga agaaatggaa gccctcgaaa cagagattat gcttgctctg
    36241 ggctatgagg atccgtacat tgccgagaaa gaataatttg ccaggcattt gactggcaga
    36301 cgtccccgcc gttggtgatt aacgacggcg ttttgattaa ctaacatgag aacccatacg
    36361 acgccatgag cgacgacaat tcacacagta gtgacacgat aagcaacaag aagggatttt
    36421 tctccctgtt actcagccaa cttttccacg gtgaaccgaa aaaccgtgac gaactgctgg
    36481 cgctgatccg tgattccggg cagaacgacc ttatcgacga agatacgcgc gatatgctcg
    36541 aaggggtgat ggacatcgca gaccaacgcg ttcgcgacat catgatcccc cgctcccaga
    36601 tgattaccct gaaacgcaac cagacgctgg acgaatgcct tgatgtcatc atcgagtccg
    36661 cccactcacg tttcccggtg attagcgaag acaaagatca cattgaaggg attctgatgg
    36721 cgaaagactt gctgccgttt atgcgcagcg atgctgaagc cttcagcatg gacaaagtgt
    36781 tacgtcaggc ggttgtcgtt cctgaaagta agcgcgtaga ccggatgctg aaagagtttc
    36841 gctctcagcg ttaccacatg gcgatcgtta ttgacgaatt cggtggggtt tccggtctgg
    36901 tgaccattga agacatcctg gaactgattg ttggtgagat tgaagacgag tatgacgaag
    36961 aagatgatat cgacttccgt cagctgagtc gtcatacctg gaccgtgcgc gcactggctt
    37021 ccattgaaga cttcaacgaa gcgttcggca cccactttag cgatgaagaa gtcgacacta
    37081 tcggtggtct ggtgatgcag gcgtttgggc atcttccggc gcgtggcgaa actatcgaca
    37141 tcgacggtta ccagttcaaa gtggcgatgg ccgatagtcg gcgtattatt caggttcatg
    37201 tcaaaatccc ggatgactca ccccagccaa agctggatga ataaaaccga aactggatag
    37261 ataactacat ggcttttgcc tcattaattg aacgccagcg tattcgcctg ctgctggcgt
    37321 tattattcgg tgcctgcgga acgctggcct tctctcctta cgacgtctgg cctgcggcga
    37381 ttatttcgct gatggggctt caggcgttga cctttaaccg tcgcccactc cagtctgccg
    37441 ctattggctt ttgctgggga tttggcctct ttggcagcgg tattaactgg gtctatgtca
    37501 gcatcgcgac ctttggcggg atgcctggcc cggttaacat cttcctggtg gtgctgctgg
    37561 cggcgtattt gtcgctgtat accggactgt ttgctggcgt gctgtcgcgt ctgtggccga
    37621 aaaccacctg gctgcgcgtg gcgattgcca cccctgctct ctggcaagtg accgagtttc
    37681 tgcgcggttg ggtgctgact ggcttcccgt ggttacagtt cggctatagc cagattgatg
    37741 gcccgttaaa agggctggca ccgctaatgg gcgtggaagc cattaacttc ctgctgatga
    37801 tggttagtgg cctgctggca ctggcgttgg tcaaacgcaa ctggcgtccg ctggtggtgg
    37861 ccgtcgtgct gtttgcccta cccttcccgc tgcgttacat ccaatggttt accccgcaac
    37921 cggagaaaac cattcaggtt tcgatggttc agggcgatat tccgcaatcg ctgaaatggg
    37981 acgaaggcca gcttcttaat acgctaaaga tttactacaa cgcaacggca ccgctgatgg
    38041 gcaaatcatc gttgattatc tggccggagt cggcgataac cgatctggaa attaatcagc
    38101 aaccgttcct caaagcactg gacggtgagt tgcgtgataa aggtagctcg ctggtaaccg
    38161 ggattgtcga cgcgcgtctc aataagcaga accgctacga tacctacaac accatcatca
    38221 cgctgggtaa aggtgcaccg tacagctacg aatcagccga tcgctataac aaaaaccatc
    38281 tggtgccgtt tggcgagttt gtcccgctgg agtcgattct gcgtccgtta gcaccgttct
    38341 ttgatctgcc gatgtcgtcg ttcagccgtg ggccatatat ccagccgccg ctgtcggcaa
    38401 atggtattga gcttactgcg gctatttgct acgagatcat tctcgccgag caagtgcgcg
    38461 ataacttccg cccggatacc gactacctgc tgactatctc caacgatgcg tggtttggta
    38521 aatctattgg tccatggcaa cacttccaga tggcgcgaat gcgtgcgctg gagctggcgc
    38581 gcccactgtt gcgcagcacc aacaacggca ttacggcggt gattggcccg cagggtgaga
    38641 ttcaggcgat gatcccgcag ttcgcccgcg aggtgttaac cactaacgtg acgccgacca
    38701 ccggactcac accatacgca cgtaccggca actggccgct gtgggtgctg acggcactgt
    38761 ttggttttgc tgctgtgttg atgagtctgc gtcagcgacg taaataatcc cttcttatcg
    38821 tgccggaata catcccatat ttcggcacgc tcaccggatg cggcatcagt gcattatccg
    38881 gataacaata ttctcctatc caactttggc acatctattg ctttgttata caaggcaaag
    38941 cttgaaccag catcagtaca acccggttgc accacagcaa tccaccggta gcaccaaaag
    39001 agtgcatttt aagaattttg cttcagaatg gtgcggtgca cctcgcaaaa acaaacaata
    39061 aaacatcaat atctttacat ttagccaaac taaatgttac ctaacaggca caacactgca
    39121 caataaagtt gcagacgata acaacacaaa cactcacaac gggtatccat gcgttcttaa
    39181 cgcagaagat aaaggtgttg gatatgcaat tacgtaaacc tgccacagca atcctcgccc
    39241 tggcgctttc cgcaggactg gcacaggcag atgacgccgc cccggcagca ggcagtacgc
    39301 tggacaaaat cgccaaaaac ggtgtgattg tcgtcggtca ccgtgaatct tcagtacctt
    39361 tctcttatta cgacaatcag caaaaagtgg tgggttactc gcaggattac tccaacgcca
    39421 ttgttgaagc agtgaaaaag aaactcaaca aaccggactt gcaggtaaaa ctgattccga
    39481 ttacctcaca aaaccgtatt ccactgctgc aaaacggcac tttcgatttt gaatgtggtt
    39541 ctaccaccaa caacgtcgaa cgccaaaaac aggcggcttt ctctgacact attttcgtgg
    39601 tcggtacgcg cctgttgacc aaaaagggcg gcgatatcaa agattttgcc gacctgaaag
    39661 gcaaagccgt agtcgtcact tccggcacta cctctgaagt tttgctcaac aaactgaatg
    39721 aagagcaaaa aatgaatatg cgcatcatca gcgccaaaga tcacggtgac tctttccgca
    39781 ccctggaaag cggtcgtgcc gttgccttta tgatggatga cgctctgctg gccggtgaac
    39841 gtgcgaaagc gaagaaacca gacaactggg atatcgtcgg caagccgcag tctcaggaag
    39901 cctacggttg catgttgcgt aaagacgatc cgcagttcaa aaagctgatg gatgacacta
    39961 tcgctcaggt gcagacctcc ggtgaagcgg aaaaatggtt tgataaatgg ttcaaaaatc
    40021 caattccgcc gaaaaacctg aacatgaatt tcgaactgtc agacgaaatg aaagcactgt
    40081 tcaaagaacc gaatgacaag gcactgaact aattacaaga accaggggcg gaaattccag
    40141 ccctctcgat tgttacgtag cacggacaga ctatacgcct gatggtcgtt ccccatcggg
    40201 cctgaaaacc gcaatacgct gggtaacaat cttcgagggt agcagttaac gctgctaccc
    40261 tcttttttct ggagtagatt tatgtctata gactggaact ggggtatttt tttacaacaa
    40321 gccccgttcg gcaataccac ctatcttggt tgggtctgga gtggttttca ggtgacgatc
    40381 gctttgtcga tctgcgcctg gattatcgct ttcctcgtcg gttcattttt cggcatttta
    40441 cgtaccgttc caaaccgttt tctctccggt ctgggtacgt tgtatgtcga actgttccgc
    40501 aacgtgccgt tgattgtgca attctttacc tggtatctgg tgatcccgga gctgctgccg
    40561 gagaaaatcg gcatgtggtt taaggccgag ctggatccca atattcagtt tttcctttca
    40621 tccatgctct gcctggggct gtttaccgcc gcccgtgttt gcgaacaggt acgcgccgct
    40681 attcagtcgc taccgcgtgg gcagaagaat gccgcgctgg cgatgggcct gacgctaccg
    40741 cagacttatc gatacgtact gctgcctaat gcttatcgcg ttatcgtccc gccgatgact
    40801 tccgagatga tgaacctggt gaaaaactcc gccatcgcct cgaccatcgg tctggtggat
    40861 atggcggcgc aggcgggtaa attgctcgac tactcggctc acgcctggga gtcgtttacc
    40921 gccatcacgc ttgcgtatgt gttgattaac gcttttatca tgctggtgat gacgctggtt
    40981 gaacgtaaag ttcgcctgcc cggcaacatg gggggcaaat aatgtacgag tttgactgga
    41041 gttccattgt tccttccctg ccatatctgc tcgacgggct ggtgatcacc ctgaaaatca
    41101 ccgtcacggc agtcgtgatt ggtattttgt ggggtaccat gctggcggtg atgcgcttgt
    41161 ccagcttcgc gcctgtttcg tggtttgcca aagcctacgt taacgtattc cgctcgattc
    41221 ctttagtcat ggttttgctg tggttttact tgattgtgcc gggatttctg caaaatgtgc
    41281 tgggattatc gccaaaaaat gatattcgtt taatttcggc gatggtggcg ttttcgatgt
    41341 ttgaagcggc ctactattca gagattatcc gtgccggtat tcaaagtatt tcccgtggtc
    41401 agtcgagcgc cgcgctggcg ttggggatga ctcactggca gtcgatgaaa ctgattattc
    41461 tgccgcaggc tttccgcgcg atggtgccgc tgctgctcac tcagggcatc gtactgttcc
    41521 aggatacctc gctggtgtat gtgttatctc tggccgattt cttccgtacc gcctcaacca
    41581 ttggtgagcg tgatggtacg caggtcgaga tgatcctgtt tgccggattt gtttatttcg
    41641 ttattagcct tagcgcgtcg ttgttggtca gctacttgaa aagaaggaca gcataatgat
    41701 taccctgaaa aatgtttcaa aatggtatgg tcactttcag gtgctgaccg actgctcaac
    41761 cgaagtgaaa aaaggcgaag tggtggtggt ttgcggcccg tctggttccg gcaaatcaac
    41821 gctgattaaa accgtcaacg gcctcgaacc agtgcagcaa ggtgaaatca ccgtcgatgg
    41881 tatcgtggtt aacgacaaga aaaccgatct ggcaaagctg cgctcccgcg tcgggatggt
    41941 attccagcat ttcgagctgt tccctcatct gtcgattatc gaaaacctga ccctggcgca
    42001 ggtgaaagtg cttaaacgcg ataaagcgcc cgctcgtgaa aaagcattga aattgctgga
    42061 gcgcgtggga ctttccgcac atgccaataa atttccggcg cagctttccg gcggtcagca
    42121 gcagcgcgtg gcaatcgctc gcgcattgtg tatggctcct attgcgatgc tgttcgacga
    42181 accgacatcg gcgctggatc cggagatgat caacgaagtg ctggacgtga tggtggaact
    42241 ggcgaacgaa ggaatgacca tgatggtggt gacccacgaa atgggctttg cccgcaaagt
    42301 ggcgaatcgg gtgatcttta tggacgaggg taaaattgtc gaagactcgc cgaaagacgc
    42361 tttcttcgat gatccgaaat cggaccgcgc aaaagacttc ctcgcgaaaa tcctgcatta
    42421 atcactctgg cgcacatacc caattgcgcg ccatctcacg ctttatcgtt tcgcccctcg
    42481 tatctgttac atcctggcgt tacccttgtc gcaaagaagc acacaacaag gagcaacaat
    42541 ggcactgcca attctgttag attgcgaccc aggtcatgat gacgctatcg caatagttct
    42601 cgcactcgcc tcaccagagc ttgatgtcaa agcaattacg tcttccgccg gaaaccagac
    42661 accagaaaaa accttacgca atgttctgcg tatgctgacc ttgcttaatc gcaccgatat
    42721 tccggtggca ggcggcgcgg taaaaccgtt aatgcgtgag ttgattatcg cggacaatgt
    42781 gcacggcgaa agcggtctcg acggcccggc attaccggaa ccgacgttcg caccgcaaaa
    42841 ctgtacggcg gtggagctga tggcgaaaac gctgcgcgaa agtgcggaac ctgtcacgat
    42901 tgtttctacc ggaccgcaaa ctaacgttgc cttgctgctc aatagccatc cggaactgca
    42961 tagcaaaatt gcccgtatcg tgattatggg cggcgcaatg gggcttggta actggacgcc
    43021 tgcggctgaa tttaatattt acgttgaccc ggaagcggca gaaattgtct tccagtcagg
    43081 gatcccggtg gtgatggccg gtctggatgt tactcataaa gcgcaaatcc acgttgaaga
    43141 caccgagcgt ttccgcgcga ttggcaaccc tgtttcaacc attgttgccg aactgctgga
    43201 tttcttcctc gaatatcata aagacgaaaa atggggcttt gtcggcgcac cgctgcatga
    43261 cccatgcacc atcgcctggc tgttgaaacc cgagttgttt accactgttg agcgctgggt
    43321 tggcgtggaa acacagggga aatataccca gggtatgacg gttgttgatt attattatct
    43381 gactggcaat aaaccgaatg ccactgtaat tgtcgatgtt gatcgtcagg gctttgttga
    43441 tttactggct gatcgtctga aattttacgc ttaacactcg tcattgatta agatcaccac
    43501 gcgttacctg ggtggtctta atctatttat ctctttttct ttcaagatat aaatgcaatg
    43561 gataatgcag aactcgccat aggtatcgat ctcggcacta ccaatagttt aattgccgtc
    43621 tggaaagacg gtgccgcgca attaattcca aataagttcg gtgaatattt aacaccatcc
    43681 ataattagca tggatgaaaa taatcatatt ttagtcggaa aaccggctgt atcacggcgt
    43741 acttcgcatc cggataaaac ggcagcgtta tttaaacgtg caatgggcag ctggcggtta
    43801 ggcagcgaca catttaacgc gccagaactg tcctctttgg tattacgctc attaaaagaa
    43861 gatgccgaag aatttctgca acgtccgatt aaagatgtgg tgatctccgt tccggcttat
    43921 ttcagtgatg aacaacgcaa gcatacccgt ttagcagcgg acttagccgg gttaaatgcg
    43981 gtacgcttaa ttaatgaacc cacagcagct gcgatggcgt atggcctgca tacccaacaa
    44041 aatacccgtt cgctggtgtt tgatctcggt ggcggcacgt ttgacgttac ggtgcttgag
    44101 tacgccacgc cggtgattga agttcacgcc tccgctggcg acaacttcct cggtggcgaa
    44161 gattttaccc atatgttggt cgatgacgtt ttaaagcgcg cggatgtcac aaggaccacg
    44221 cttaacgaga gtgaactggc agccttgtac gcctgtgtgg aagcggcaaa atgtagcaat
    44281 caatcgccaa tgcacattcg ctggtagtac caggatgaaa cgcgggaatg cgaattttac
    44341 gagaacgaac tggaagattt gtggttgccg ctgctcaatc gcttgcgagt gccgattgaa
    44401 caggcgttgc gcgatgcgcg tctgaagccg agtcaaatcg acagtctggt gctggttggc
    44461 ggcgcgtcac aaatgccgct ggtgcagcga atcgccgtgc gtctgtttgg caaattaccg
    44521 tatcaaagtt acgatccgag caccattgtc gcgctgggcg cagcaatcca ggccgcctgc
    44581 cgcttacgca gtgaagatat tgaagaagtg atcctcactg atatttgccc ttactcgttg
    44641 ggcgttgagg ttaaccgcca gggcgtttcc ggcattttct cgccgattat tgaacgaaac
    44701 accactgtgc ccgtgtcgcg tgtagaaact tattcaacca tgcacccgga acaggattca
    44761 attacggtta acgtctatca gggagaaaac cacaaagtta aaaacaacat tctggtggaa
    44821 tccttcgatg tgccgttgaa gaaaaccggg gcttatcagt cgattgatat tcgctttagt
    44881 tatgatatca acgggttgct tgaagttgac gtgcttctgg aagacggcag cgttaagtcc
    44941 agagtgatta accacagccc ggtaacattg agcgcgcagc agattgaaga gagtcggacg
    45001 cggttatccg cattgaaaat ttatccgcgc gatatgctca tcaatcgcac ctttaaagcc
    45061 aaactggaag agttgtgggc gcgggcgctg ggtgacgagc gagaagagat tggccgggtg
    45121 atcaccgatt ttgatgcggc gctgcagtca aacgatatgg cccgcgtcga tgaggtcagg
    45181 cggcgggcga gcgtttattt agccattgag acctcataat cacggctgcc ggatgcggcg
    45241 taaatgctgc atccggcatc ggcgcaaaaa acacatcgtc aactaaacaa catcgcgatt
    45301 ttgccgagag cataacctat aaccagcacc gttaaactga ttttgataat gctggtaatg
    45361 ctctgtggtt tttcaccctc atcatttttt tcttcatggt tttcaggtgt tggctcatcg
    45421 acatcttcat cctccggctc gagttttact ttcgtcaaca gcatccgggt gataatggcc
    45481 tctaatggat gagcatctcc gccttccagc cttgtattaa gacgggccag catctcttgt
    45541 ttcggaaacg cttcaagtac tacctgttcc gctacccgat acaaatccag taacggtgaa
    45601 tcttcttctg tctcgctacc gactctcgat ttccaccacg cccatgcttt gcgtttgaag
    45661 aataacgact caataaacga ttgacctaac ggcgataatt ttccgctatg taaaatttcc
    45721 gcccataaca gccgcgtccg ggcgctctca ccaggaacat atttcagcgg ttgaccttgc
    45781 gcatctacca tgctccgcgc ttcgataacc atcactaata acgcgaaata atccgggcaa
    45841 tgacgcttgc tccactgcaa caacaaatta tcagcttgtg tagatgggta actttcccag
    45901 tacgcgccga gatcagcaag caggctttcc ccctcgccgc agtacaaacg ttgtgcacac
    45961 tggtaatacc gggcatcttc gctttctggt tcttccgctt gccactgttt ggctacagga
    46021 atgagttcca ccatacctgt ttgcaccgag ctgtaccagc gcaacagttt gcgatgtaac
    46081 tttgcatcat caggaatgaa ccacggtcct tccatcgcca aaaacgcagt gacatagtca
    46141 ggatggtaac ggcagcaacg ttccagcgcg aagtaatatt caacagtctg gttctgtaac
    46201 gcgacgggat attccagaag gctaaggaaa tcgaaacaat caccagcctg aatggcctca
    46261 agaaaagcct ccctttcttc ctcatccatt ccttcgctgt cattaaacga atgccagttc
    46321 aaacgctctg ccaacaaaga agcacaattt aatgacagat atcgcgcttc tatagcgatt
    46381 gcacacagcg gccagcgtaa ttgatcgaca tcctccatgt tccaggtatt taattgctga
    46441 ataaatttct gccaggcgga gggctgaaaa cgatcacttt ctgaatccag taatgtcctg
    46501 aatgcacgta gtatttcatg ttcagcggtg gcatcttttt cttcatcatc tgcttcctcg
    46561 acaggattta ccgccagtcg cagcgcctct tcataggcct ggcgtaatgc tttaaacccc
    46621 tgcggatcgg tttcgggatg acacaacggt aagcgagcaa gataagcctg gcggataatg
    46681 tctatttgcg tcgtgctttc aatttcgagg atttgccaac atgttttcat aggtcgctca
    46741 catccttaag cgttaccggg cattcaggct ggacagttaa tggctgccag ggaagcgtgg
    46801 catagaaacg gtcgcttccc gctgccagct cttcgaacat ccgactcccg gcgctatcgt
    46861 atttgtacag ggttagcgcc tcgcgtagcg aagcgttgtc ttcgcactga gaagattgcc
    46921 agtagagacg ccctagtgaa taggcagaaa aatagtgcat ccagctgtga tagtaatggt
    46981 gagctcgtgc atagacgcgc gattgcagcc ataaactctc ttcttccgtc agccagttgt
    47041 tgagcacacc ggcccgtgac agaaacccca ttcgaacata atcccaggct ttgattcccc
    47101 cttctccaca gcacatagcg gtttgggcaa cgaattgggt ataaatcaac cctctctccg
    47161 agccaccagc aattaatgct ttccactctt caggggaaga gcgttgccat gtcagatata
    47221 agccagctaa atcattggca tgaccatcat cagtcatctg ataaatagtc tgaattaaat
    47281 catcacgatt atttattccc caggatgatg agagatcgaa ttcttttatc ccggtataca
    47341 tttttggcga gtcatgcgcg ggtttgcaac tctgattaag tatttccatg ggcgcagaaa
    47401 gcgcaaaaag aagatattgt tcttccttat tcatttttta ataatccctt aagtgcatta
    47461 actgactact tttttattat tgatgagtat aaacttgacg ctttcaaaat aaaaagaaaa
    47521 tcgaagcatt cacacatgaa taaaaaatta atgtatatat tcgcaatttt tatagttgca
    47581 gcaattacct gtattagcca acccaagaaa acgatgttgc gtgataaagc catggtgaat
    47641 tatgcctttg attatttaag atcaccaggc agtctaccgt taaccacggc agccacggag
    47701 ctttccgcga ttcatggtca ctcaacatcg caatatcgcc ttggcgaatt ttatcttcat
    47761 ggtagcgacg gtaaaccact ggattataca caggcgagat actggtatga gcaatcagca
    47821 gaacaggaaa atccgcgcgc acaaagcaaa ctgaggtgga tctacctgaa aggcctggga
    47881 gtcaaacccg atacccgtaa agcaattctc tggtataagg aagcagctga acaagggtat
    47941 gctcatgctc aatatacttt gggtttgatc tacagaaatg gcactggtat taatgttaac
    48001 cattatgaat ctcaaaaatg gttaaaactg gccgccaaac aacattacaa aaatgcggaa
    48061 agattacttg ccggacttcc cgcacattaa cgtcgactgt cacccgaaga gatggagtat
    48121 tttccctatc aaaccggcaa agataaaaat gtagaaaata attttgatga cgcccagaca
    48181 gcccagcccg ccatcatcaa cgggcattgg ctgtggttgc ggctccggct cgacggtttc
    48241 ctgaccggta aaattcgccg cctgaattaa taatcgacga ataattaatg cttccagtgg
    48301 acaacgatct tctgcatcaa ggcggctcac aatcggcatt aatgattctt ctgtcggttg
    48361 gctggcctgc actaattgtt tggcaactaa atataatggc cataagggat gttctgtatc
    48421 acgcgtgagt gagatccgcg gcttacgtcc ttcgtgcaaa accatctcga ccagtgaacg
    48481 ccccagcgca gagagttttt catcgctgag tatttcgtat aaatgaaaac gcgtacgggc
    48541 gctgtcgccg gggacataaa gtagcggttt tccctgatcg ttaaccagat cacgcgcttc
    48601 aatcatcatc accactaatg ggtaataata gacccgatgc tgacggcacc attgcaacat
    48661 taaagcatcc gcctgggtgg agggatactc gcgccagtag taacaaagtt ctgccagcag
    48721 gctttcgcct tctccacaat agacgcgttg tgcgtattca taataacgcg catcttcatt
    48781 gtcaggttca gcttcccgcc acgaccgggc gtaatccagg agttcgggga ttcccaggcg
    48841 gcttgcgcta taccagcgga gtaaatttct gtgcagtttt gcatcatcgg ggataatcac
    48901 tgcgcgatgt tgcgctaaat acaatgtcag ccattctgga tgcgatgacc aaatccgagc
    48961 gaacatttgg taaaaatcga tggtgtcatt ttgcacggca actggcagat gcagaatggt
    49021 ctggaagtta aaaacattcc ccttcgcaat ggcatataaa aaggattcca gttcttcttc
    49081 gtctatttcc ccgacgtttt cctcctgcaa ccaccgcaat ctttctgcta ataacaccac
    49141 gcactcgaag gataaatggg cagtgttcat ggcgattgtg cacagcgacc agcgtaattc
    49201 atcaatctct tccatcgagc aataatttaa ttgctgaatg aatcgctgcc aggcggagga
    49261 cagaaaacgt tcgctatcag aggcaagtaa cgcgcgaaag gcgagcagaa tttcatgttc
    49321 tgctacctca tattcttctg gttgccaaac agatttagca ggcgactgcg caatccgtag
    49381 cgcctcctca tacgcctgac gaagttgttt aaaaccctgc ggatcggttt ctggatgaaa
    49441 ggacggtaac agcgccagat aagcgcggcg gataatatcg acgtcagtcg tttcctctat
    49501 atcgaggatc ttccagcaat ttttcataag tcgctcatat ccttaagcgt ttccgggcat
    49561 gtggggtagt cagccagtgg ttgccaggac aaggtagggt aatacgcatc atcactggca
    49621 aataattgat aaaacatatc attgccggaa tcgtcgtatt ctttacgcgc taagaattcg
    49681 cgtaataaag gcagatgatc atcttcggga gactgccaat attgccgacc aaaggtataa
    49741 ccggcaaagt attgccgcca gttactataa taacgtagag cccgtagatg aatacgcgac
    49801 tggatccata gactttcttc ttcactcaac cagttattaa gtacgcccat tcgacttaaa
    49861 aaacccatcc ggacataatc ccatgcttta attcctccct caccacagca ttcggctgtg
    49921 ctggcgacaa attgcgcata agcctgacct tgctcattta attcagcaag atagtcacgc
    49981 cattcacatg gagaataacg aaaccagcgg tgataaaacc ctgccaaccg agcggcatga
    50041 ccgttctcga tcattcggtg aattatgcgc agcaggtcat cacggtcatc aattccccat
    50101 gaggaagaaa ggtcaaaaaa gcatgtacct aaatacattt tgggggatga atgcgaaggt
    50161 aaacagcatt cattgcgtat ttccatcgga gtggaaagtg caaacaatat tttttgcgac
    50221 cccatgtcca tataatttat ccttaaacat tcatttaatg aatgtaaact acatttcgag
    50281 gctcagttat ttatactttt ctgacgcaac ataatacgca taaaataagg cttacgcaaa
    50341 aatggaatac ataaaaatat aatatatttt atctacaacc ataaggaaat gataatgatt
    50401 tttacgtcaa gttgctgcga taatttatca atagatgaga ttatcgaacg tgctgaaaaa
    50461 ggcgattgcg aggctcaata tattgttggg ttttattata atcgcgatag cgcaattgac
    50521 tctccggacg acgaaaaggc cttttactgg ctgaagctgg ccgctgagca aggtcattgt
    50581 gaagcacagt attccttagg gcaaaagtat accgaggata aaagccgtca taaagataat
    50641 gagcaagcca tcttctggct gaaaaaagct gccctacaag gccatacttt cgcttccaac
    50701 gcccttggct ggatactgga tcgtggagaa gaccccaact ataaagaagc ggttacctgg
    50761 tatcagatag ccgcggagag cggaatgtct tatgcgcaaa ataatcttgg atggatgtac
    50821 agaaatggca acggagtcgc acaagactat acgctggcat ttttctggta caaacaagct
    50881 gcattacaag gccatagtta cgcacaagac aatctggccg atctttataa agacggagaa
    50941 ggcgttgctc aaaacaagac actcgccgca ttctggtatt tgaaaagcgc acagcagggt
    51001 aatcggcacg cccagtttca aattgcatgg gattataacg ctggcgaagg ggtggaccag
    51061 gactataagc aagcgatgta ctggtatctg aaggctgccg ctcaggaaag cgtcggcgct
    51121 tacgtcaaca tcggttatat gtataaacac ggacaaggcg ttgagaaaga ttatcaggct
    51181 gcctttgaat ggtttacgaa agccgctgaa tgcaatgacg ccactgcctg gtataacctg
    51241 gccattatgt atcactacgg agaaggaaga cctgtcgatc tccgacaggc tctcgacctg
    51301 tatcgtaaag ttcagtcatc cggaaccaga gatgtcagtc aggaaattcg tgagactgaa
    51361 gctttactgt agaaataaaa acaagggccg gggaatttca ttctgcccat attctcttta
    51421 ttgctgtgcg aaatcagact attacggctc aaacgggcgt ctctggaact ggtcataacc
    51481 acatttcggg catagcgtca gcacttccgg tgtgtagatc gggagatgga agtgacattt
    51541 ctcgcagacc agatttccca gcccgaccac ttctccgctg tgataaaccc catgatgatt
    51601 gaggtcctgg aaaatttcgc gccattcaag ctgcgtttta tcggtgatgt ctgccagctc
    51661 ctgccacaag ctttctttaa tcacccgcat aaagacgctg tcagattctt ctttcaggct
    51721 ctcttcatag ctcatggcga actcttccag gtcacgtctg acagctcgcg tcagctcatc
    51781 gatctcggtt cgcgttaact cccctgtttt tattacgcgc tcgcgcgcct gttccaccag
    51841 tgcgtcgata tcacgttcgc cattgcgcag gcgttcgttc agtgacgcga ccagttcacg
    51901 gtaatattga gcaaccttgt tcatcgtttc atctcccggg tcgttaactg actataataa
    51961 tagacgttct ttgttccttg ctttgctaat acgcccacaa actctgtaaa ttaatgtacg
    52021 aataatccgg gcatcatttc aacgtcgagt gcgcgaaagg ctgttttgac gcggacgttt
    52081 gggctatgct atgcggatct gaaaaaccac ctcaacgcta catttgtagc cgtattgaaa
    52141 acaggaccac tggctgccat gcaagagcaa taccgcccgg aagagataga atccaaagta
    52201 cagcttcatt gggatgagaa gcgcacattt gaagtaaccg aagacgagag caaagagaag
    52261 tattactgcc tgtctatgct tccctatcct tctggtcgac tacacatggg ccacgtacgt
    52321 aactacacca tcggtgacgt gatcgcccgt taccagcgca tgctgggcaa aaacgttctg
    52381 cagccgatcg gctgggacgc gtttggtctg cctgcggaag gcgcggcggt gaaaaacaac
    52441 accgctccgg caccgtggac gtacgacaac atcgcgtata tgaaaaacca gctcaaaatg
    52501 ctgggctttg gttatgactg gagccgcgag ctggcaacct gtacgccgga atactaccgt
    52561 tgggaacaga aattcttcac cgagctgtat aaaaaaggcc tggtatataa aaagacttct
    52621 gcggtcaact ggtgcccgaa cgaccagacc gtactggcga acgaacaagt tatcgacggc
    52681 tgctgctggc gctgcgatac caaagttgaa cgtaaagaga tcccgcagtg gtttatcaaa
    52741 atcactgctt acgctgacga gctgctcaac gatctggata aactggatca ctggccagac
    52801 accgttaaaa ccatgcagcg taactggatc ggtcgttccg aaggcgtgga gatcaccttc
    52861 aacgttaacg actatgacaa cacgctgacc gtttacacta cccgcccgga cacctttatg
    52921 ggttgtacct acctggcggt agctgcgggt catccgctgg cgcagaaagc ggcggaaaat
    52981 aatcctgaac tggcggcctt tattgacgaa tgccgtaaca ccaaagttgc cgaagctgaa
    53041 atggcgacga tggagaaaaa aggcgtcgat actggcttta aagctgttca cccattaacg
    53101 ggcgaagaaa ttcccgtttg ggcagcaaac ttcgtattga tggagtacgg cacgggcgca
    53161 gttatggcgg ttccgggtca cgaccagcgc gactacgagt ttgcctctaa atacggcctg
    53221 aacatcaaac cggttatcct ggcggctgac ggctctgagc cagatctttc tcagcaagcc
    53281 ctgactgaaa aaggcgtgct gttcaactct ggcgagttca acggtcttga ccatgaagcg
    53341 gccttcaacg ccatcgccga taaactgact gcgatgggcg ttggcgagcg taaagtgaac
    53401 taccgcctgc gcgactgggg tgtttctcgc cagcgttact ggggcgcgcc gattccgatg
    53461 gtgacgctgg aagacggtac cgtaatgcca accccggacg accagctgcc ggtgatcctg
    53521 ccggaagatg tggtgatgga cggcattacc agcccgatta aagcagatcc ggagtgggca
    53581 aaaactaccg ttaacggtat gccagcgctg cgtgaaaccg acactttcga cacctttatg
    53641 gagtcctcct ggtactatgc gcgctacact tgcccagagt acaaagaagg tatgctggat
    53701 tccgaagcgg ctaactactg gctgccggtg gatatctaca ttggtggtat cgaacacgcc
    53761 attatgcacc tgctctactt ccgcttcttc cacaaactga tgcgtgatgc aggcatggtg
    53821 aactctgacg aaccagcgaa acagttgctg tgtcagggta tggtgctggc agatgccttc
    53881 tactatgttg gcgaaaacgg cgaacgtaac tgggtttccc cggttgatgc tatcgttgaa
    53941 cgtgacgaga aaggccgtat cgtgaaagcg aaagatgcgg caggccatga actggtttat
    54001 accggcatga gcaaaatgtc caagtcgaag aacaacggta tcgacccgca ggtgatggtt
    54061 gaacgttacg gcgcggacac cgttcgtctg tttatgatgt ttgcttctcc ggctgatatg
    54121 actctcgaat ggcaggaatc cggtgtggaa ggggctaacc gcttcctgaa acgtgtctgg
    54181 aaactggtgt acgagcacac agcaaaaggt gatgttgcgg cactgaacgt tgatgcgctg
    54241 actgaagatc agaaagcgct gcgtcgcgat gtgcataaaa ctatcgctaa agtgaccgat
    54301 gatatcggcc gtcgtcagac cttcaacacc gcaattgcgg cgattatgga gctgatgaac
    54361 aaactggcga aagcaccgac cgatggcgag caggatcgcg ctctgatgca ggaagcactg
    54421 caggccgttg tccgtatgct caacccgttc accccgcaca tctgcttcac gctgtggcag
    54481 gaactcaaag gcgaaggcga tatcgacaac gcgccgtggc cggttgctga cgaaaaagcg
    54541 atggtggaag actccacgct ggtcgtggtg caggttaacg gtaaagtccg tgccaaaatc
    54601 accgttccgg tggacgcaac ggaagaacag gttcgcgaac gtgctggcca ggaacatctg
    54661 gtagcaaaat atcttgatgg cgttactgta cgtaaagtga tttacgtacc aggtaaactc
    54721 ctcaatctgg tcgttggcta agcgcgggag gaagcgtgcg atatctggca acactgttgt
    54781 tatctctggc ggtgttaatc accgccgggt gtggctggca tctgcgtgac accacgcagg
    54841 ttccttccac tatgaaggtc atgatcctgg actcaggcga tccgaacggg ccattaagcc
    54901 gtgcggtgcg taaccagtta cgtctgaatg gtgtcgagtt gcttgataaa gaaaccacgc
    54961 gtaaggacgt tccatccctg cgtttgggta aagtgagcat cgcgaaagat accgcatcgg
    55021 tattccgtaa cggtcaaaca gcagagtatc agatgatcat gacggtgaat gcgaccgtgt
    55081 tgatccccgg ccgtgatatc tacccgatta gcgccaaagt cttccgttca ttcttcgata
    55141 acccgcaaat ggcgttagcg aaagataacg aacaagacat gatcgtaaaa gagatgtacg
    55201 accgtgctgc cgaacagctg attcgtaagc tgccaagcat ccgtgctgcg gatattcgtt
    55261 ccgacgaaga acagacgtcg accacaacgg atactccggc aacgcctgca cgcgtctcca
    55321 ccatgctggg taactgatga ttcggttgta cccggaacaa ctccgcgcgc agctcaatga
    55381 agggctgcgc gcggcgtatc ttttacttgg taacgatcct ctgttattgc aggaaagcca
    55441 ggacgctgtt cgccaggtag ctgcggcaca aggattcgaa gaacaccaca ctttttccat
    55501 tgatcccaac actgactgga atgcgatctt ttcgttatgc caggctatga gtctgtttgc
    55561 cagtcgacaa acgctattgc tgttgttacc agaaaacgga ccgaatgcgg cgatcaatga
    55621 acaacttctc acactcaccg gacttctgca tgacgacctg ctattgatcg tccgcggtaa
    55681 taaattaagc aaagcgcaag aaaatgccgc ctggtttact gctcttgcga atcgcagcgt
    55741 gcaggtgacc tgtcagacac cggagcaggc tcagcttccc cgctgggttg ctgtgcgcgc
    55801 aaaacagctc aacttagaac tggatgacgc ggcgaatcag gtgctctgct actgttatga
    55861 aggtaacctg ctggcgctgg ctcaggcact ggagcgttta tcgctgctct ggccagacgg
    55921 caaactgaca ttaccgcgcg ttgaacaggc ggtgaatgat gccgcgcatt tcaccccttt
    55981 tcattgggtt gatgctttgt tgatgggaaa aagtaagcgc gcgttgcata ttcttcagca
    56041 actgcgtctg gaaggcagcg agccggttat tttgttgcgc acattacaac gtgaactgtt
    56101 gttactggtt aacctgaaac gccagtctgc ccatacgcca ctgcgtgcgt tgtttgataa
    56161 gcatcgggta tggcagaacc gccggggcat gatgggcgag gcgttaaatc gcttaagtca
    56221 gccgcagtta cgtcaggccg tgcaactcct gacacgaacg gaactcaccc tcaaacaaga
    56281 ttacggtcag tcagtgtggg cagagctgga agggttatct cttctgttgt gccataaacc
    56341 cctggcggac gtatttatcg acggttgata tgaaatcttt acaggctctg tttggcggca
    56401 cctttgatcc ggtgcactat ggtcatctaa aacccgtgga aacgctggcg aatttgattg
    56461 gtctgacgcg ggtcacaatc atccctaata atgttcctcc gcatcgtccc cagccggaag
    56521 cgaacagcgt gcagcgtaaa cacatgcttg aactggcgat tgccgacaag ccattattta
    56581 ctcttgatga acgcgagcta aagcgcaatg ccccctctta cactgcgcaa acactgaaag
    56641 agtggcggca ggaacaagga ccggacgtgc cgctggcgtt tattattggt caggattcat
    56701 tgctgacctt tccgacctgg tacgaatacg aaacgatact cgacaatgca catttgatcg
    56761 tctgtcggcg tccaggttac ccacttgaaa tggcgcaacc gcaataccag caatggctgg
    56821 aagatcattt gacacataac ccggaagatc ttcaccttca gcctgccggt aaaatttatc
    56881 tggctgaaac gccgtggttt aacatctcgg cgaccatcat ccgcgaacgt ttgcaaaacg
    56941 gtgaatcgtg tgaggattta ttgccggaac cggtattgac ttacattaac caacaaggct
    57001 tgtatcgctg atacctgtcg ttacggaggg catgatgcga ctgtggttaa ttcgtcatgg
    57061 cgaaacacaa gcgaatgtcg atggtctgta cagcggtcat gcgcccacac ccctgaccgc
    57121 acgcggtatt gagcaagcgc aaaatctgca tacactgcta gatgatgttt cctttgacct
    57181 ggttttatgc agtgaactgg aacgggcaca gcataccgcg cgactggttc tcagtgaccg
    57241 ccagctcccc gttcatatca tccctgaact caacgaaatg ttttttggcg actgggagat
    57301 gcgacatcat cgcgacctca tgcaagaaga tgccgaaaac tatagcgcgt ggtgcaatga
    57361 ctggcagcat gctatcccca cgaatggtga aggatttcag gcattttcgc aacgtgtgga
    57421 acgctttatc gcaaggctta gtgaatatca gcactatcag aatattttaa tcgtcagcca
    57481 tcagggcgta ctgagtctgt taattgctcg tttaattggc atgcctgccg agtctatgtg
    57541 gcattttcgc gttgaccagg ggtgttggag cgccattgat atcaaccaaa aattcgcgac
    57601 gctacgcgtc ctcaatagcc gtgccatcgg ggtcgagaac gcatgacttt tctgtttttt
    57661 tacgggtaag ccgcaacgac cattgacagc cccgggcagg ctgatattct ccgcagccag
    57721 actttttccg ccagacacga ctttgtagaa attgttttac aaaaatggcg atgcaatctg
    57781 cggcgcgggg tgggatgata gcccactttc gaaagccgat tcggcgacaa ttgtcccgaa
    57841 atcgcctctg gttcaggtat actgacagac catttttatc tatttgattc acccaggggg
    57901 aaaacttgca gggtaaagca ctccaggatt ttgttatcga caaaattgat gacctcaaag
    57961 gtcaggacat catcgcctta gacgttcaag gcaaatccag catcaccgac tgcatgatca
    58021 tctgtacggg tacgtccagc cgtcatgtta tgtccattgc tgaccacgtt gtgcaggagt
    58081 ctcgcgcagc gggcctgtta ccgctcggcg tagaaggtga aaacagcgcc gactggattg
    58141 tcgtggattt gggcgatgtg attgtccatg tcatgcagga agagagccgt cgcctgtatg
    58201 aactggaaaa actctggagt taatgcgtga agctgcaact tgtcgccgtg ggaacgaaaa
    58261 tgccggactg ggtacaaacc ggttttaccg agtacctgcg tcgttttccg aaagatatgc
    58321 ccttcgagct gattgaaatt ccggccggca aacgcggcaa gaatgcggac atcaagcgca
    58381 tactcgacaa agagggtgag cagatgttgg cggccgcagg caaaaaccgc attgtcaccc
    58441 tcgatattcc aggcaagccc tgggatacgc cgcagttagc cgctgagctg gaacgctgga
    58501 agctggatgg tcgcgacgta agtctactga ttggcgggcc tgaagggttg tcgcctgcct
    58561 gtaaagcggc ggctgagcag agctggtcgc tgtcggcgct taccctcccc catccgctgg
    58621 ttcgcgtgct ggtcgcagag agtctgtacc gggcgtggag catcaccacc aaccatcctt
    58681 atcaccgtga gtgataaggg agctttgagt agaaaacgca gcggatgaaa ctacagaact
    58741 cttttcgcga ctatacggca gagtccgcgc tgtttgtgcg ccgggcgctg gtcgcctttt
    58801 tggggatttt gctgctgacc ggcgtgctta tcgccaacct gtataatctg caaattgttc
    58861 gctttaccga ctaccagacc cgctctaatg aaaaccgcat taagctggtg cctatcgcgc
    58921 ccagccgcgg cattatctac gaccgtaacg gtatccctct ggccctcaac cgcactatct
    58981 accagataga aatgatgccg gagaaagtcg ataacgtgca gcaaacgctg gacgctttgc
    59041 gcagcgtggt agatctgacc gatgacgata ttgctgcatt ccgaaaagag cgcgcacgtt
    59101 cacaccgttt cacctctatt ccggtgaaaa ctaacctaac cgaagtacaa gtagctcgct
    59161 ttgccgtcaa tcagtaccgt tttccgggtg tcgaagttaa aggctataaa cgtcgttact
    59221 atccttacgg ttcggcgttg acccacgtca tcggctatgt gtcgaaaatc aacgataaag
    59281 acgtcgaacg cctgaataat gacggcaaac tggccaacta tgcggcaacg catgatattg
    59341 gtaagctggg cattgagcgt tactatgaag acgtgctgca cggtcagacc ggttatgaag
    59401 aggttgaagt taacaaccgt gggcgtgtta tccgccagtt aaaagaagta ccaccgcaag
    59461 ccggacacga tatttacctg acgctggatc tcaaactcca gcaatatatt gaaacgctgc
    59521 tggcgggtag ccgcgcagct gtggttgtta ccgaaccgcg tacaggtggg gtgctggcgc
    59581 tggtttccac gcctagttat gacccaaacc tgtttgttga cggtatctcc agcaaagatt
    59641 attccgcctt gctgaacgac ccgaatacac cgctggtgaa ccgcgccaca cagggggttt
    59701 atcctcccgc gtctacagtt aaaccctatg tggcggtttc ggcattaagc gccggggtga
    59761 tcacgcgcaa tacgacgctg tttgacccag gctggtggca actaccaggg tcggaaaaac
    59821 gttatcgtga ctggaaaaaa tggggccacg ggcgtctgaa tgtcacaaga tcgctggaag
    59881 aatctgcgga taccttcttc tatcaggtgg cctacgatat ggggatcgat cgcctctccg
    59941 aatggatggg taaattcggt tatggtcatt acaccggtat cgacctggcg gaagaacgtt
    60001 ccggcaacat gcctacccgc gaatggaaac agaaacgctt taaaaaaccg tggtatcagg
    60061 gtgacaccat tccggttggt atcggccagg gttactggac agcgacccca atccagatga
    60121 gtaaggcact gatgatcctg attaatgacg gtatcgtgaa ggttccgcat ttgctgatga
    60181 gcaccgccga agacggcaaa caggtgccat gggtacaacc gcatgaaccg cccgtcggcg
    60241 atattcattc cggttactgg gagctggcga aagacggtat gtacggtgtt gctaaccgcc
    60301 ctaatggtac ggcgaataaa tactttgcca gcgcaccgta caaaattgcg gcgaaatctg
    60361 gtaccgctca ggtcttcggt ctgaaagcga acgaaaccta taatgcgcac aaaattgccg
    60421 agcgtttacg tgaccacaaa ctgatgaccg cctttgcgcc atacaacaat ccgcaagtgg
    60481 ctgtcgccat gattctggag aacggcggcg cgggtccggc ggttggtacg ctgatgcgcc
    60541 agatcctcga ccacattatg ctgggtgata acaacaccga tctgcctgcg gaaaatccag
    60601 cggttgccgc agcggaggac cattaatcat gacggataat ccgaataaaa aaacattctg
    60661 ggataaagtc catctcgatc ccacaatgct gctgatttta ctggcattgc tggtttacag
    60721 cgccctggtt atctggagcg ccagcggtca ggatattggc atgatggagc gtaaaatcgg
    60781 ccaaatcgcg atgggtctgg tcatcatggt ggtgatggcg caaattcctc cacgcgttta
    60841 tgaaggctgg gccccctatc tctatatcat ctgtattatt ttgctggtgg cggtagatgc
    60901 tttcggagcc atctctaaag gcgctcaacg ctggctggac ctcggtattg ttcgttttca
    60961 gccgtcggaa attgccaaaa tagccgtacc actgatggtt gcgcgcttta tcaaccgcga
    61021 cgtttgcccg ccatcgttga agaatactgg catcgcgctg gtgctgatat ttatgcccac
    61081 gctgctggtg gctgcacagc ctgacctggg aacatcaatc ctcgttgcgc tttccggtct
    61141 gtttgtactg ttcctctctg gccttagctg gcgtctgatt ggcgtcgcag tagtgctggt
    61201 agcggcgttc attccgattc tgtggttctt cctgatgcat gattaccagc gccagcgcgt
    61261 aatgatgctc ctggacccgg aatcagaccc actcggcgcg ggctatcaca ttattcagtc
    61321 taaaattgct attggctccg gcggattacg cggcaaaggc tggctgcacg gcactcagtc
    61381 acagcttgaa tttctccccg aacgccatac tgactttatc ttcgcggtac tggcggaaga
    61441 gctgggatta gtgggcattc tgattctgct cgctctctac attctgctga tcatgcgcgg
    61501 gatgtggata gccgccagag cgcaaaccac ctttggtcgc gtcatggctg gtggcttaat
    61561 gctgatatta ttcgtttatg tcttcgtaaa tattggtatg gtaagcggta ttctgccggt
    61621 tgtaggggtt ccgctcccac tggtcagtta tggaggatcg gcgctaattg tgctgatggc
    61681 tgggttcggg attgtaatgt caatccacac ccacaggaaa atgttgtcga aaagcgtgta
    61741 agaggtgcgc aatgcgtaag cagtggctcg ggatctgcat cgcggcagga atgctcgcgg
    61801 catgtacaag cgatgatggt cagcaacaga cggtaagtgt accgcagcct gcggtatgta
    61861 acggccccat agttgaaatt agcggggcgg acccgcgttt cgaaccactg aacgcgacgg
    61921 caaatcagga ttaccagcgc gacggtaaaa gctacaaaat cgtgcaggat ccgtctcgat
    61981 ttagccaggc gggactggcg gcaatctatg atgccgaacc aggcagtaac ctgacggcct
    62041 ctggcgaagc tttcgatccg acaaagctga cggcggccca tccaacgctt ccgatcccca
    62101 gctacgccag aatcactaac ctggctaacg ggcgaatgat cgtggtgcgc attaatgatc
    62161 gcggccctta cggcaacgac cgcgttattt cgctttctcg cgcggcagct gaccgtctta
    62221 acacgtcaaa caacaccaaa gttcgtatcg atccgattat tgtcgcccag gatggttcgc
    62281 tttctggtcc tggtatggct tgtaccacag tcgccaaaca gacttacgcc ctgcctgcac
    62341 ctcccgattt aagcggtggc gcgggaacaa gttcagtgtc tggcccgcag ggtgacattc
    62401 ttccggtcag taattcgacg ctaaaaagcg aagatccgac cggcgcgccg gtaaccagta
    62461 gcggtttcct cggcgctcca acgaccttag cgcctagtgt actggaaggc agcgaaccga
    62521 cgcctgcgcc acagcccgtt gttacagctt cgtcgacaac gcctgcaacc tcgcctgcaa
    62581 tggtgacacc gcaagccgcc tcgcaaagcg ccagcggcaa ctttatggtg caagtcgggg
    62641 ccgtaagcga tcaggctcgt gcgcaacagt accaacagca actgggacag aagttcggcg
    62701 tccccggtcg cgtaactcaa aatggcgcgg tctggcggat ccagcttggc ccattcgcca
    62761 gcaaagccga agccagtacc ttgcagcaac gtttgcaaac cgaagcccaa ttacagtcat
    62821 ttattactac cgcgcagtag cgtaaagcag gcatctgatg tgtcaatttc tgtaaatgac
    62881 gttaacaaag tcacgcggaa agtcagatgc ctgccggtag tgcatttgct atagtagggc
    62941 acttttttaa ttccatcacg gatgtcgtag ttcagaccat gaataccatt ttttccgctc
    63001 gtatcatgaa gcgcctggcg ctcaccacgg ctctttgcac agcctttatc tctgctgcac
    63061 atgccgatga cctgaatatc aaaactatga tcccgggtgt accgcagatc gatgcggagt
    63121 cctacatcct gattgactat aactccggca aagtgctcgc cgaacagaac gcagatgtcc
    63181 gccgcgatcc tgccagcctg accaaaatga tgaccagtta cgttatcggc caggcaatga
    63241 aagccggtaa atttaaagaa actgatttag tcactatcgg caacgacgca tgggccaccg
    63301 gtaacccggt gtttaaaggt tcttcgctga tgttcctcaa accgggcatg caggttccgg
    63361 tttctcagct gatccgcggt attaacctgc aatcgggtaa cgatgcttgt gtcgccatgg
    63421 ccgattttgc cgctggtagc caggacgctt ttgttggctt gatgaacagc tacgttaacg
    63481 cactgggcct gaaaaatacc cacttccaga cggtacatgg tctggatgct gatggtcagt
    63541 acagctccgc gcgagatatg gcgctgatcg gccaggcatt gatccgtgac gtaccgaatg
    63601 aatactcgat ctataaagaa aaagaattta cgtttaacgg tattcgccag ctgaaccgta
    63661 acggcctgtt atgggataac agcctgaatg tcgacggcat caaaaccgga cacactgaca
    63721 aagcaggtta caatcttgtt gcttctgcga ctgaaggcca gatgcgcttg atttctgcgg
    63781 tgatgggtgg acgtactttt aaaggccgtg aagccgaaag taaaaaactg ctaacctggg
    63841 gcttccgttt cttcgaaacc gttaacccac tgaaagtagg taaagagttc gcctctgaac
    63901 cggtttggtt tagtgattct gatcgcgctt cgttaggggt tgataaagac gtgtacctga
    63961 ccattccgcg tggtcgcatg aaagatctga aagccagcta tgtgctgaac agcagtgaat
    64021 tgcatgcgcc gctgcaaaag aatcaggtcg tcggaactat caacttccag cttgatggca
    64081 aaacgatcga gcaacgcccg ctggttgtgt tgcaagaaat cccggaaggt aacttcttcg
    64141 gcaaaatcat tgattacatt aaattaatgt tccatcactg gtttggttaa aaaattgaac
    64201 acttgaaagt gtaatttccg tccccatata ctaagcatca gtaaaaaaac tcccgccttc
    64261 tggcgggagt tgctatttaa ttacgttacg ccggagctga catgaaaacc aaacttaacg
    64321 aactgcttga attccctact ccttttactt acaaagttat ggggcaggcg ttacctgagc
    64381 tggttgatca ggtggttgaa gtggtacagc gccatgcgcc aggtgactac accccaacgg
    64441 taaaaccaag cagcaaaggc aactaccact cggtatctat cactatcaac gccactcata
    64501 tcgagcaggt tgaaacactg tatgaagaac tgggcaaaat cgatattgtc cgcatggttc
    64561 tgtaactcgc ttctccgtta cccggctcct ggtcgggtaa ctcccctctc ctcgctgtga
    64621 tatacttttc ccccactttt actcattctc cacggagatg ccgttttgta tcaggataaa
    64681 attcttgtcc gccagctcgg tcttcagcct tacgagccaa tctcccgggc tatgcatgaa
    64741 ttcaccgata cccgcgataa tagtaccctt gatgaaatct ggctggtcga gcactatccg
    64801 gtattcaccc aaggtcaggc aggaaaagcg gagcacattt taatgccggg tgatattccg
    64861 gtgatccaga gcgatcgcgg tgggcaggtg acttatcacg ggccgggaca acaggtgatg
    64921 tatgtgttgc ttaacctgaa acgccgtaaa ctcggtgtgc gtgaactggt gaccttgctt
    64981 gagcaaacag tggtgaatac cctggctgaa ctgggtatag aagcgcatcc tcgggctgac
    65041 gcgcctggtg tctatgtcgg ggaaaagaaa atttgctcac tgggtttacg aattcgacgc
    65101 ggttgttcat tccacggtct ggcattaaac gtcaatatgg atctttcacc atttttacgt
    65161 attaatcctt gtgggtatgc cggaatggaa atggctaaaa tatcacaatg gaaacccgaa
    65221 gcgacgacta ataatattgc tccacgttta ctggaaaata ttttagcgct actaaacaat
    65281 ccggacttcg aatatattac cgcttaattc catacatcaa tggcccaatt tacactgggt
    65341 cattactcat aatacctgct aatgctttac atctccttcg cgtttgtata ttttctttat
    65401 cacttgcaga aatgcggtgc cacgccttgc tccgtaactc accccattga ttaacctatt
    65461 gaaatagttg attatggtga gcaaaagcgt tccgttccaa ttagacataa gattattgta
    65521 attttttaaa tattattttc ccatgaataa aattggagta agtgcgtgga tagtaataat
    65581 caaattgaac cctgtttaag cagaaagtcg tcagaaggta aaccacaaat atttacaacc
    65641 ttacgaaata ttgatcttaa ccttctgact atttttgaag ctgtatatgt acataaaggg
    65701 atcgttaatg cagcgaaagt gcttaatctg accccctcgg caatcagtca gtctattcag
    65761 aaactgcgcg ttatattccc tgacccattg tttattcgca aaggccaggg tgtcactcct
    65821 accgcatttg cgatgcatct acatgagtat atcagtcagg gccttgagtc cattcttggc
    65881 gcgctggata tcgaaggaag ctatgataag caacgaacga taactattgc gaccactccc
    65941 tcggtcggag ccctggtcct tcctgtcatc tatcgggcga ttaaaactca ctatccgcag
    66001 cttttactgc gcaacccgcc catcagcgac gcggaaaacc aactcagtca gtttcaaacc
    66061 gatctcatca tcgataacat gttttgcacc aatcgtacgg tgcaacatca tgttctgttc
    66121 accgacaata tggtgttaat ttgccgcaag ggaaatccac tactctcttt agaagatgac
    66181 agagagacta tcgacaacgc tgcgcatgta cttctgttac cggaagggca aaatttcagc
    66241 ggtctgcggc agagagttca agagatgttt ccggaccggc aaatcaattt caccagctac
    66301 aacattttga caatcgctgc actggttgcc aacagtgaca tgttagcgat tattcccagc
    66361 cgtttttata acctgtttag tcgttgctgg ccgctggaaa aactgccttt tccgtcctta
    66421 aatgaggagc aaatagattt ctctatccac tacaacaaat tcatcctgcg tgatcccatc
    66481 ctgcacggag taatagatgt tatccgtaat gcattttgaa aaagtattgt tacagccaga
    66541 tcacagcgca gaacgataaa atggcacaat ccaacaacaa ttttacattt tcgcgaccgc
    66601 tttggctgct ttcaggtccg tttcaatgat atactgccag tcgttaattc aaaaatagtt
    66661 gataattaca acaatctatt gaattgaaac gctttccttc gtaattcgca actggaacac
    66721 gcacgctatg agtaaaccca ttgtgatgga acgcggtgtt aaataccgcg atgccgataa
    66781 gatggccctt atcccggtta aaaacgtggc aacagagcgc gaagccctgc tgcgcaagcc
    66841 ggaatggatg aaaatcaagc ttccagcgga ctctacacgt atccagggca tcaaagccgc
    66901 aatgcgcaaa aatggcctgc attctgtctg cgaggaagcc tcctgcccta acctggcgga
    66961 atgcttcaac cacggcacag caacgtttat gatcctcggc gctatttgta cccgccgttg
    67021 tccgttctgt gatgttgccc acggtcgccc ggtagctcct gatgccaatg aaccagtgaa
    67081 actggcgcag accattgccg atatggcgct gcgttatgtg gttatcacct ccgttgaccg
    67141 tgatgacctg cgcgatggcg gtgcccaaca ctttgcggat tgtattactg ccattcggga
    67201 aaaaagcccg caaatcaaaa ttgaaactct ggtgccggat ttccgcggtc gaatggatcg
    67261 tgctctggat attctgactg caacgccccc agatgtgttc aaccataacc tggaaaacgt
    67321 accgcgtatt taccggcagg tacggcctgg cgcagattac aactggtcgc tgaagctgct
    67381 ggaacgcttt aaagaagcgc atccggaaat cccgaccaag tctggtctga tggtgggtct
    67441 gggtgaaacc aatgaagaaa ttattgaggt aatgcgcgac ctgcgccgtc atggtgtgac
    67501 gatgttaacg ctggggcaat atttgcagcc aagccgccat cacctgccgg ttcaacgtta
    67561 cgttagcccg gatgagttcg acgaaatgaa agccgaagcg ctggcgatgg gctttaccca
    67621 tgctgcatgc ggtccgtttg tccgctcttc ttaccacgcc gatttgcagg cgaaaggaat
    67681 ggaagttaag taatcctgta ataattactt acatctgtct gataaaaaac ccgctttttg
    67741 aagcgggttt tttgtatcga acaagatatt gagggagcgt cctgctcgcc acgtcactct
    67801 ttatgagaga gcttttcagc ctgaagatca acgtctgcgc cttttttcgc cgcagcatcg
    67861 tcatcattca tcgccttctt gaacccttta atggccgctc caaggtctcc gcccagcgta
    67921 cgtaacttct tagtcccaaa cagcagaacg accagcgccg caactaccag cagtttggta
    67981 atactaatct cacccataga taccttcttg acatataacc ggcaacagaa cgcgccttaa
    68041 aaaccagaga atattaacgg tcattcgacg cgcgcacaca atagcaatct gtaacaaggt
    68101 gaatcaagcg ccgagtaaaa aaacatcata ataattgcgg cggcgcaaag cgacggttgt
    68161 ttaagatggg cagttgcgcg cgcacctgac gcacacgttc gggcgtcact tccgccataa
    68221 tgagtgcagg catttctgac gctgccgcaa tggtgacgcc aaagggatct ataatccggc
    68281 tttgcccgat atttttgttc ccgcactccc ccgccgccac catataacag gtggtatcca
    68341 gcgcacgagc ggcaagcaac gttgaccagt gatgctcttt gagcggccca cgaacccagg
    68401 cggcaggaag taccaggatt tcagctccct gtaaagcctg tgccagcgcc agctctggaa
    68461 agcgtaagtc ataacaggtc atcagaccga ccttcatccc ttccacctcc agtaacggag
    68521 ctatttcatt accagcatca acatggcgtg attcctgaat ggcaaatgca tcatagagat
    68581 gcagtttggc ataacgggcg acgatgtttc ctgcctgaag tgccaccagc atattccatg
    68641 cgcgccccgg cgttgaagga agatgaatcg tcagaattgt cgtcatcatg ttacgtttac
    68701 tttctcgccg taaacgtccg aggaattcgc cttccagcag ctgtgctgat ttaaccgata
    68761 gatctgcatc atgatcatcg cgcgccagca atgcttccgg caggacaaac agcgatacgt
    68821 cgttttccgc cgcctgcgcc atcaacgagg cacaaatctc agcgttcttt tcccacacag
    68881 atgtaacagc aaactgtcct gcagcaacca gcattgccac ctcctgtcag agtcgtaaaa
    68941 ttcgccaacg acgattattc ggcgttacac ttatcactca tacaaatcaa atagcaggat
    69001 tttgcagtgt tacaacttct tttagcagtt tttattggcg gtggtacggg aagcgtagcg
    69061 agatggctgt taagtatgcg atttaacccg ctgcatcagg cgattccgtt ggggacgctg
    69121 gcagcaaacc tgattggggc attcatcata ggaatgggat tcgcctggtt cagcaggatg
    69181 acgaacattg atccagtgtg gaaagtatta atcaccaccg gattttgtgg cggtctaaca
    69241 accttctcaa cattttcggc agaagtggtg tttttgttac aagagggccg ctttggctgg
    69301 gcattactga acgttttcgt caaccttctg gggtcttttg ccatgaccgc actggcattc
    69361 tggctgtttt cggcctcaac cgtacactaa agaccaaaaa aaacccgctg attaagcggg
    69421 ttttgaattc ttgctgacgt atcttacaga gcgattacgt ttgcagcaga agggcctttg
    69481 gcaccgttag tgatttcgaa ctctacgcgc tgaccttcag caagagtttt aaaaccatta
    69541 gtctggattg cagagaagtg tacgaacacg tctttgctgc cgtcttccgg agtaatgaaa
    69601 ccgaatcctt tggactcatt aaaccactta acgttacctt taatcttaga catcaaaatt
    69661 acctttacat gaaaaataga cacaaatgct gtgtcggtta tcagtacacc aattgtggta
    69721 cgctttgtcc agtgcaactt tactaaaaag tgataaaagt cgcgttattt ttgatgttgc
    69781 ttattttagt tattgatttt aaagaagtta ctaaaacttc atttgtctca aaactgaaag
    69841 cgcatccagg caaagtacac attgccattg ttgtaggtac ccggaatgta ggtcatctga
    69901 aaagtcactg ggccataacc cacggaggcc aatggcagta gaaccgggag agggatgtaa
    69961 ttccagttat cgcgtgccgt tacgccagcg gtgaatccca gacctaaatg aaaattttca
    70021 tccgccagcg gtcgccaggt actttcccat ccgtatccgg caatcggttc ccatttgttc
    70081 cacgagtcct taaatgccat ggcatacagg ccatgccagt ttcctttttc atcccaacgc
    70141 gacaggccaa aaccgccacc ccacggtcgc tcgttatagc gatcggtttt ttctttgtcg
    70201 taagcgaaac gtgcatgcca ggtgatggca ggaatatata aatcataatg ttcaggctgt
    70261 tgccaggttt gtgcaatatt ttctctaaac gttgtcatcc tctcatctgc gttagcaaaa
    70321 actttaccaa cgctgattaa ctgaataaaa acaaaggaaa agatagcgac atatttactc
    70381 acgttcattt gtgaccataa aacatttatc aaaaatctac tactagcata gcaaagctac
    70441 aattaacata accttaatag acccaacata aagaataatc tgaatagctg ctctatttat
    70501 ttccgcttgt tcaagttaaa tatttccgcg catcgattaa agatgatgcg tcgttttctg
    70561 cgatgggaat agtcaaaaaa gaaaaaccaa gtcttttttg atgacaaaat gcaatcaagg
    70621 aaaaattatt ttattttttt aacgagatac aacaatcatc ttaacgaagt atataatata
    70681 tatcgtcaac catgctcata ttagattatt ttttgagcgc acagccgcag ctaacaccat
    70741 tgcaattaac aaatttgcat caatccacca tcaatttaca cacattatta tgtgataatt
    70801 gccaaccgct aaatatgcgt tttgttatct atgtataaaa acagcaactt caatgtctta
    70861 atggcagttt ttcttgattt taatcagcat tcatcgccaa tttattgggc atattttttc
    70921 cttaagcttt aggaagtttt tattatttac tttgggcctg gagacaggaa aaattatgct
    70981 gacattcatt gaactcctta ttggggttgt ggttattgtg ggtgtggctc gctacatcat
    71041 taaagggtat tccgccactg gtgtgttatt tgtcggtggc ctgttattgc tgattatcag
    71101 tgccattatg gggcacaaag tgttaccgtc cagccaggct tcaacaggct acagcgccac
    71161 ggatatcgtt gaatacgtta aaatattact aatgagccgc ggcggcgacc tcggcatgat
    71221 gattatgatg ctgtgtggct ttgccgctta catgacccat atcggcgcga atgatatggt
    71281 ggtcaagctg gcgtcaaaac cattgcagta tattaactcc ccttacctgc tgatgattgc
    71341 cgcctatttt gttgcttgtc tgatgtcact ggccgtctct tccgcaaccg gtctgggtgt
    71401 tttgctgatg gcaaccctgt ttccggtgat ggtaaacgtt ggtatcagtc gtggcgcagc
    71461 agctgccatt tgtgcctccc cggcggcgat tattctcgca ccgacttcag gggatgtggt
    71521 gctggcggcg caggcttccg aaatgtcgct gattgacttc gccttcaaaa cgacgctgcc
    71581 tatctcaatt gctgcaatta tcggcatggc gatcgcccac ttcttctggc aacgttatct
    71641 ggataaaaaa gagcacatct ctcatgaaat gttagatgtc agtgaaatca ccaccactgc
    71701 ccccgcgttt tatgccattt tgccgttcac gccgatcatc ggtgtactga tttttgacgg
    71761 taaatggggt ccgcaattac acatcatcac tattctggtg atttgtatgc tgattgcctc
    71821 cattctggag ttcctccgca gctttaatac ccagaaagtt ttctctggtc tggaagtggc
    71881 ttatcgcggg atggcagatg cgtttgctaa cgtggtgatg ctgctggttg ccgctggggt
    71941 attcgctcag gggcttagca ccatcggctt tattcaaagt ctgatttcta tcgctacctc
    72001 gtttggttcg gcgagtatca tcctgatgct ggtattggtg attctgacca tgctggcggc
    72061 agtcacgacc ggttcaggca atgcgccgtt ttatgcgttt gttgagatga tcccgaaact
    72121 ggcgcactct tccggcatta acccggcgta tttgactatc ccgatgctgc aggcgtcaaa
    72181 cctgggtcgt accctatcac ccgtttctgg cgtagtcgtt gcggttgccg ggatggcgaa
    72241 aatctcacca tttgaagtcg taaaacgcac ctcggtgccg gtgcttgttg ggctagtgat
    72301 tgttatcgtt gctacagagc tgatggtgcc aggaacggca gcagcggtca cagacaagta
    72361 aatagtaatg ccggaggcaa gtttcctccg gctttatcat gtcacccact gtggtatatg
    72421 cgttgtggtc tgccaacttt gccgtgaaca atttcagcaa taatcagatg gcggctggcg
    72481 caatattcaa gataacgcct ggcagtggtg cggctgatgg tcagtgcctg cgccaccgtt
    72541 tctgccgtat gttgcacacc aggctcttta aacagttttc gcaccgcgtt tagcgtcaag
    72601 gggtcaatgc cggtcggcag ttcgtctttg ggttcaccgc gagcataggc attaaacatc
    72661 tcatcaattt gcttctggct ggcgctatca atactttcca gcatatgttt acgctggcgg
    72721 aaacgggtta gcgtttgccc cagtgtattg attcacttga agtacgaaaa aaccgggaga
    72781 acattggatt attcgggatc tgatgggatt agatttggtg gggcttgcaa gcctgtagta
    72841 caaattttag gcgttaacca atgaaacgcg aaagatagta aaaaattgct tttgtttcat
    72901 tgaaaaaacg aaaaacaaaa acactataaa tcatttcaat catagcttca aaaaacgttc
    72961 aaaaccgata acaaccaggc tgtaaccaaa tgactcatat cacaaatcag ctcatgccgt
    73021 ttaggtatgt tacatgtgtg attatgtgag gtgaagtatg ttttagctgg ctcatggttg
    73081 ttatacggct tttttcgcat cttgtggttc caacaaagga gctacaggct cttcctgttt
    73141 atgaacacca tacattgaca aaatctcttg gcgagtttct ggaggtaatt tatgcagcgc
    73201 caaagctgtt tgtagtgttt ggcgatcaat acccagcgtt gccaataggg tgtcaacgga
    73261 gatggagggg cgttcttgca ttaatccgga aagcccatat ttagccatgt ttagctctat
    73321 ctctcggact tcattttctc ctagtttatt gattaacagg gagaacattt tcaatgtatc
    73381 agcattcatt tcttcgatag caattttttg catagccggt attgccggga tatattcaac
    73441 cccaggcccg cgacgccctt cttgtgaacg cttcggccat ccttctttct ttgctcgttt
    73501 atgcaccccc tgtattgtcc caggcattcc atccagttca gtaatttgtt tgggtgtcat
    73561 ccactggttt atctcatttg cgcttttcat aaactaagtt taccagattt atctatataa
    73621 aaatcaatgg attacaaaat aaaccacatt aactgatata aaccggtttt gtttatttgg
    73681 taatgtataa aacagataat caagacgaat tatttgtttt ggtaaccctt tatcatacgc
    73741 aatatggttg gtgtgcgaaa gatgagtaac gattggcatc gggccgacgt gattgcggcc
    73801 ctcaaaaaaa caggaacccc tctggcaaaa ttgtccagac gttcaggtta ttcaaccggg
    73861 acgcttgcaa atgcattagt caggccatgg cctaaaggtg aacgcattat tgctgacgcc
    73921 ttaaacatga aacctgagga aatttggcct agtagatatc taagggatca caatgagaga
    73981 gtggatatca gcaattgaat gcatgggttt atctggttta cctggttcat taatgggggt
    74041 tcacaaaaga gctcagagag aaggatggct caagagaaga agagaaggaa ttagaggggt
    74101 tgcttacgaa taccatatat cttctcttcc taacattgct cgagctgaag ttttgctcca
    74161 acaaggaaaa tttaaaacca gtcagggatg ttttgaaatc gcccgcccca cgctggaagc
    74221 tcatgattat gatcgtgagg cactatggag caaatgggat aaagccagcg attcccagcg
    74281 cagcctggct gaaaaatggt tgccctcaat tcaggccaca gacgaaatgc tgaaccaggg
    74341 gatttcaacg aaaacggctt ttgcgaccgt tgcagggcat taccaggtca gcgcatccac
    74401 tttgcgggac aagtattacc aggtacagaa gtttgcgaag cctgactggg cggctgcact
    74461 tgttgatgga cgtggagcat cccgtcgcaa tgttcacaaa agtgaatttg acgaggatgc
    74521 ctggcagttt ctgattgcag attatctgcg accggaaaaa cccgccttcc gcaaatgtta
    74581 tgagcgtctg gaactggcag cccgcgagca tggctggagt attccctccc gtgccacggc
    74641 ctttcgccgg attcagcaac tggacgaggc aatggttgtt gcctgtcgtg aaggtgaaca
    74701 tgcactgatg catctgatac cggcacagca gcgaactgtg gaacacctgg acgccatgca
    74761 gtggatcaac ggcgacggtt atctgcataa cgtctttgta cgctggttta acggtgatgt
    74821 gatccgtccg aaaacatggt tctggcagga tgtgaaaacc cgaaaaattc tgggctggcg
    74881 ctgcgatgtg agcgagaaca ttgattcaat tcgcctctcg ttcatggatg ttgtgactcg
    74941 ctacagtatc ccggaggatt ttcacatcac cattgataac acccgtggtg ctgcgaataa
    75001 atggctgacg ggaggcgcgc ccaatcgcta ccgctttaag gtaaaagagg acgatccaaa
    75061 aggactgttt ttactgatgg gtgcgaaaat gcactggaca agcgttgttg ccggtaaagg
    75121 ctggggccag gcaaaacctg ttgaacgtgc tttcggtgtt ggtgggcttg aggaatacgt
    75181 tgataagcat ccggcactgg ctggcgcata tacggggcca aatccgcagg caaaacctga
    75241 taactatggc gaccacgctg ttgatgcaga gctgtttctg aaaacctttg ccgaaggtgt
    75301 ggcgatgttc aatgccagaa caggccgtga aacagaaatg tgcgggggca aactctcgtt
    75361 tgatgatgtt ttcgagcgtg aatacgccag aacgattgtg cgtaagccaa ccgaagaaca
    75421 aaaacggatg ctgttactgc ctgccgaggc ggtgaacgtt tcacgcaaag gcgagtttac
    75481 gcttaaagtt ggcggctccc ttaaaggcgc gaaaaacgtt tattacaaca tggcattaat
    75541 gaatgccgga gtgaaaaaag ttgtggtcag atttgatcca cagcagttac acagcacggt
    75601 ttattgag
//
LOCUS       DAEEUE010000005        72215 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.005, whole genome shotgun sequence.
ACCESSION   DAEEUE010000005 DAEEUE010000000
VERSION     DAEEUE010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72215
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.005"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            28..1149
                     /gene="roxA"
                     /locus_tag="KLK84_000346"
                     /gene_synonym="ycfD"
     CDS             28..1149
                     /gene="roxA"
                     /locus_tag="KLK84_000346"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HBE2007817.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(1198..2424)
                     /gene="pepT"
                     /locus_tag="KLK84_000347"
     CDS             complement(1198..2424)
                     /gene="pepT"
                     /locus_tag="KLK84_000347"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBE2007818.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            2674..3810
                     /gene="potA"
                     /locus_tag="KLK84_000348"
     CDS             2674..3810
                     /gene="potA"
                     /locus_tag="KLK84_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HBE2007819.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            3794..4651
                     /gene="potB"
                     /locus_tag="KLK84_000349"
     CDS             3794..4651
                     /gene="potB"
                     /locus_tag="KLK84_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HBE2007820.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            4648..5442
                     /gene="potC"
                     /locus_tag="KLK84_000350"
     CDS             4648..5442
                     /gene="potC"
                     /locus_tag="KLK84_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HBE2007821.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            5439..6485
                     /gene="potD"
                     /locus_tag="KLK84_000351"
     CDS             5439..6485
                     /gene="potD"
                     /locus_tag="KLK84_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HBE2007822.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(6641..7462)
                     /gene="cobB"
                     /locus_tag="KLK84_000352"
     CDS             complement(6641..7462)
                     /gene="cobB"
                     /locus_tag="KLK84_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBE2007823.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(7478..8389)
                     /gene="nagK"
                     /locus_tag="KLK84_000353"
     CDS             complement(7478..8389)
                     /gene="nagK"
                     /locus_tag="KLK84_000353"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBE2007824.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(8418..9662)
                     /gene="lolE"
                     /locus_tag="KLK84_000354"
     CDS             complement(8418..9662)
                     /gene="lolE"
                     /locus_tag="KLK84_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HBE2007825.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(9662..10363)
                     /gene="lolD"
                     /locus_tag="KLK84_000355"
     CDS             complement(9662..10363)
                     /gene="lolD"
                     /locus_tag="KLK84_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HBE2007826.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(10356..11555)
                     /gene="lolC"
                     /locus_tag="KLK84_000356"
     CDS             complement(10356..11555)
                     /gene="lolC"
                     /locus_tag="KLK84_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HBE2007827.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            11817..12890
                     /locus_tag="KLK84_000357"
     CDS             11817..12890
                     /locus_tag="KLK84_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HBE2007828.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            13018..16464
                     /gene="mfd"
                     /locus_tag="KLK84_000358"
     CDS             13018..16464
                     /gene="mfd"
                     /locus_tag="KLK84_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBE2007829.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            16607..17569
                     /gene="ldtC"
                     /locus_tag="KLK84_000359"
     CDS             16607..17569
                     /gene="ldtC"
                     /locus_tag="KLK84_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HBE2007830.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(17652..17864)
                     /locus_tag="KLK84_000360"
     CDS             complement(17652..17864)
                     /locus_tag="KLK84_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBE2007831.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            18149..18781
                     /gene="comR"
                     /locus_tag="KLK84_000361"
     CDS             18149..18781
                     /gene="comR"
                     /locus_tag="KLK84_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HBE2007832.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(18843..19382)
                     /locus_tag="KLK84_000362"
     CDS             complement(18843..19382)
                     /locus_tag="KLK84_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBE2007833.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(19609..20913)
                     /gene="ndh"
                     /locus_tag="KLK84_000363"
     CDS             complement(19609..20913)
                     /gene="ndh"
                     /locus_tag="KLK84_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HBE2007834.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISTAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(21321..21863)
                     /locus_tag="KLK84_000364"
     CDS             complement(21321..21863)
                     /locus_tag="KLK84_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBE2007835.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(21886..22911)
                     /gene="nagZ"
                     /locus_tag="KLK84_000365"
     CDS             complement(21886..22911)
                     /gene="nagZ"
                     /locus_tag="KLK84_000365"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HBE2007836.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(22922..23746)
                     /gene="thiK"
                     /locus_tag="KLK84_000366"
     CDS             complement(22922..23746)
                     /gene="thiK"
                     /locus_tag="KLK84_000366"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HBE2007837.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(23727..24368)
                     /gene="lpoB"
                     /locus_tag="KLK84_000367"
     CDS             complement(23727..24368)
                     /gene="lpoB"
                     /locus_tag="KLK84_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HBE2007838.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(24383..24760)
                     /locus_tag="KLK84_000368"
     CDS             complement(24383..24760)
                     /locus_tag="KLK84_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HBE2007839.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(24763..25122)
                     /gene="hinT"
                     /locus_tag="KLK84_000369"
     CDS             complement(24763..25122)
                     /gene="hinT"
                     /locus_tag="KLK84_000369"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HBE2007840.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            25253..25434
                     /locus_tag="KLK84_000370"
                     /pseudo
     CDS             25253..25434
                     /locus_tag="KLK84_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012138106.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            25467..27652
                     /gene="fhuE"
                     /locus_tag="KLK84_000371"
                     /pseudo
     CDS             25467..27652
                     /gene="fhuE"
                     /locus_tag="KLK84_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(27712..29145)
                     /gene="ptsG"
                     /locus_tag="KLK84_000372"
     CDS             complement(27712..29145)
                     /gene="ptsG"
                     /locus_tag="KLK84_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HBE2007841.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(29440..30237)
                     /locus_tag="KLK84_000373"
     CDS             complement(29440..30237)
                     /locus_tag="KLK84_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBE2007842.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(30248..31252)
                     /gene="holB"
                     /locus_tag="KLK84_000374"
     CDS             complement(30248..31252)
                     /gene="holB"
                     /locus_tag="KLK84_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBE2007843.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(31249..31890)
                     /locus_tag="KLK84_000375"
     CDS             complement(31249..31890)
                     /locus_tag="KLK84_000375"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBE2007844.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(31880..32902)
                     /gene="yceG"
                     /locus_tag="KLK84_000376"
     CDS             complement(31880..32902)
                     /gene="yceG"
                     /locus_tag="KLK84_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HBE2007845.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(32905..33714)
                     /gene="pabC"
                     /locus_tag="KLK84_000377"
     CDS             complement(32905..33714)
                     /gene="pabC"
                     /locus_tag="KLK84_000377"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HBE2007846.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(33834..35075)
                     /gene="fabF"
                     /locus_tag="KLK84_000378"
     CDS             complement(33834..35075)
                     /gene="fabF"
                     /locus_tag="KLK84_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBE2007847.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(35162..35398)
                     /gene="acpP"
                     /locus_tag="KLK84_000379"
     CDS             complement(35162..35398)
                     /gene="acpP"
                     /locus_tag="KLK84_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBE2007848.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(35609..36343)
                     /gene="fabG"
                     /locus_tag="KLK84_000380"
     CDS             complement(35609..36343)
                     /gene="fabG"
                     /locus_tag="KLK84_000380"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HBE2007849.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(36356..37285)
                     /gene="fabD"
                     /locus_tag="KLK84_000381"
     CDS             complement(36356..37285)
                     /gene="fabD"
                     /locus_tag="KLK84_000381"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBE2007850.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(37301..38254)
                     /gene="fabH"
                     /locus_tag="KLK84_000382"
     CDS             complement(37301..38254)
                     /gene="fabH"
                     /locus_tag="KLK84_000382"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HBE2007851.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(38322..39392)
                     /gene="plsX"
                     /locus_tag="KLK84_000383"
     CDS             complement(38322..39392)
                     /gene="plsX"
                     /locus_tag="KLK84_000383"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBE2007852.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(39473..39646)
                     /gene="rpmF"
                     /locus_tag="KLK84_000384"
     CDS             complement(39473..39646)
                     /gene="rpmF"
                     /locus_tag="KLK84_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBE2007853.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(39698..40219)
                     /gene="yceD"
                     /locus_tag="KLK84_000385"
     CDS             complement(39698..40219)
                     /gene="yceD"
                     /locus_tag="KLK84_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HBE2007854.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            40418..41002
                     /gene="yceF"
                     /locus_tag="KLK84_000386"
     CDS             40418..41002
                     /gene="yceF"
                     /locus_tag="KLK84_000386"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HBE2007855.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(41114..42073)
                     /gene="rluC"
                     /locus_tag="KLK84_000387"
     CDS             complement(41114..42073)
                     /gene="rluC"
                     /locus_tag="KLK84_000387"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HBE2007856.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            42646..45831
                     /gene="rne"
                     /locus_tag="KLK84_000388"
     CDS             42646..45831
                     /gene="rne"
                     /locus_tag="KLK84_000388"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HBE2007857.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACTSPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(46027..46980)
                     /gene="flgL"
                     /locus_tag="KLK84_000389"
     CDS             complement(46027..46980)
                     /gene="flgL"
                     /locus_tag="KLK84_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBE2007858.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNKLESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(46992..48634)
                     /gene="flgK"
                     /locus_tag="KLK84_000390"
                     /pseudo
     CDS             complement(46992..48634)
                     /gene="flgK"
                     /locus_tag="KLK84_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(48700..49641)
                     /gene="flgJ"
                     /locus_tag="KLK84_000391"
     CDS             complement(48700..49641)
                     /gene="flgJ"
                     /locus_tag="KLK84_000391"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HBE2007859.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(49641..50738)
                     /gene="flgI"
                     /locus_tag="KLK84_000392"
     CDS             complement(49641..50738)
                     /gene="flgI"
                     /locus_tag="KLK84_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HBE2007860.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(50750..51448)
                     /gene="flgH"
                     /locus_tag="KLK84_000393"
     CDS             complement(50750..51448)
                     /gene="flgH"
                     /locus_tag="KLK84_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HBE2007861.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(51501..52283)
                     /gene="flgG"
                     /locus_tag="KLK84_000394"
     CDS             complement(51501..52283)
                     /gene="flgG"
                     /locus_tag="KLK84_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HBE2007862.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(52421..53172)
                     /gene="flgF"
                     /locus_tag="KLK84_000395"
                     /pseudo
     CDS             complement(52421..53172)
                     /gene="flgF"
                     /locus_tag="KLK84_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(53192..54400)
                     /gene="flgE"
                     /locus_tag="KLK84_000396"
     CDS             complement(53192..54400)
                     /gene="flgE"
                     /locus_tag="KLK84_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HBE2007863.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQD
                     QILNTRVNLR"
     gene            complement(54425..55120)
                     /gene="flgD"
                     /locus_tag="KLK84_000397"
     CDS             complement(54425..55120)
                     /gene="flgD"
                     /locus_tag="KLK84_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HBE2007864.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(55132..55535)
                     /gene="flgC"
                     /locus_tag="KLK84_000398"
                     /pseudo
     CDS             complement(55132..55535)
                     /gene="flgC"
                     /locus_tag="KLK84_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(55539..55955)
                     /gene="flgB"
                     /locus_tag="KLK84_000399"
     CDS             complement(55539..55955)
                     /gene="flgB"
                     /locus_tag="KLK84_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBE2007865.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            56110..56769
                     /gene="flgA"
                     /locus_tag="KLK84_000400"
     CDS             56110..56769
                     /gene="flgA"
                     /locus_tag="KLK84_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HBE2007866.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            56845..57138
                     /gene="flgM"
                     /locus_tag="KLK84_000401"
     CDS             56845..57138
                     /gene="flgM"
                     /locus_tag="KLK84_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HBE2007867.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            57143..57559
                     /gene="flgN"
                     /locus_tag="KLK84_000402"
     CDS             57143..57559
                     /gene="flgN"
                     /locus_tag="KLK84_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HBE2007868.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(57599..59134)
                     /gene="murJ"
                     /locus_tag="KLK84_000403"
     CDS             complement(57599..59134)
                     /gene="murJ"
                     /locus_tag="KLK84_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBE2007869.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(59301..60167)
                     /locus_tag="KLK84_000404"
     CDS             complement(59301..60167)
                     /locus_tag="KLK84_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBE2007870.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(60169..60816)
                     /locus_tag="KLK84_000405"
                     /pseudo
     CDS             complement(60169..60816)
                     /locus_tag="KLK84_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
     gene            complement(60827..61411)
                     /gene="rimJ"
                     /locus_tag="KLK84_000406"
     CDS             complement(60827..61411)
                     /gene="rimJ"
                     /locus_tag="KLK84_000406"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HBE2007871.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            61647..62855
                     /gene="mdtH"
                     /locus_tag="KLK84_000407"
     CDS             61647..62855
                     /gene="mdtH"
                     /locus_tag="KLK84_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HBE2007872.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            62919..63566
                     /gene="grxB"
                     /locus_tag="KLK84_000408"
     CDS             62919..63566
                     /gene="grxB"
                     /locus_tag="KLK84_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HBE2007873.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            63700..64260
                     /locus_tag="KLK84_000409"
     CDS             63700..64260
                     /locus_tag="KLK84_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBE2007874.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            64366..65412
                     /gene="pyrC"
                     /locus_tag="KLK84_000410"
     CDS             64366..65412
                     /gene="pyrC"
                     /locus_tag="KLK84_000410"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBE2007875.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            65486..65731
                     /gene="dinI"
                     /locus_tag="KLK84_000411"
     CDS             65486..65731
                     /gene="dinI"
                     /locus_tag="KLK84_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HBE2007876.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            66021..66275
                     /gene="bssS"
                     /locus_tag="KLK84_000412"
     CDS             66021..66275
                     /gene="bssS"
                     /locus_tag="KLK84_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HBE2007877.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            66390..67508
                     /gene="solA"
                     /locus_tag="KLK84_000413"
     CDS             66390..67508
                     /gene="solA"
                     /locus_tag="KLK84_000413"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HBE2007878.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            67556..67669
                     /locus_tag="KLK84_000414"
     CDS             67556..67669
                     /locus_tag="KLK84_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HBE2007879.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            67930..68496
                     /locus_tag="KLK84_000415"
     CDS             67930..68496
                     /locus_tag="KLK84_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HBE2007880.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            68500..69075
                     /locus_tag="KLK84_000416"
     CDS             68500..69075
                     /locus_tag="KLK84_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HBE2007881.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(69117..70169)
                     /locus_tag="KLK84_000417"
     CDS             complement(69117..70169)
                     /locus_tag="KLK84_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBE2007882.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            70394..71314
                     /gene="lpxL"
                     /locus_tag="KLK84_000418"
     CDS             70394..71314
                     /gene="lpxL"
                     /locus_tag="KLK84_000418"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HBE2007883.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            71486..71683
                     /locus_tag="KLK84_000419"
     CDS             71486..71683
                     /locus_tag="KLK84_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBE2007884.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGADFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            71707..>72215
                     /locus_tag="KLK84_000420"
     CDS             71707..>72215
                     /locus_tag="KLK84_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBE2007885.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPGFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVS"
BASE COUNT        17574 a        19181 c        17938 g        17522 t
ORIGIN      
        1 gcagatacca gcctgtggat gcttaacatg gaataccaac tcactcttaa ctggcccgat
       61 tttcttgaac gtcactggca gaaacgcccg gtggtgttaa aacgcggctt taataatttt
      121 attgacccga tctctccaga cgagttggcg ggtctggcga tggaaagcga agttgacagt
      181 cgactggtca gtcaccagga tggcaaatgg caggtcagcc acggcccgtt cgaaagctac
      241 gatcatctcg gtgaaaccaa ctggtcatta ctggtacagg cagtaaacca ctggcatgag
      301 ccgaccgccg cgctgatgcg accgttccgt gaactaccgg actggcgtat tgatgatctg
      361 atgatttctt tttctgtacc tggcggcggc gtcggcccgc atctcgatca gtacgacgtg
      421 tttatcattc agggtaccgg acgtcgtcgc tggcgagtgg gcgaaaagct gcaaatgaaa
      481 cagcactgcc cacatccgga tctgttacag gtcgatccgt tcgaagccat catcgatgaa
      541 gagctggagc ctggtgatat tctttatatt ccgccaggat tcccgcatga aggctacgcg
      601 ctggaaaatg cgatgaacta ttccgtgggt tttcgcgcgc caaatacgcg ggaattaatt
      661 agcggatttg ccgattatgt gctgcaacgt gaactgggcg gcaactacta cagcgatccg
      721 gatgttccac ctcgtgctca tcctgcggat gttctgccgc aagagatgga taaactgcgt
      781 gagatgatgc tcgaattgat caaccagccg gaacacttta agcaatggtt tggcgagttt
      841 atatcccagt cacgtcatga actggatatc gcgccgccgg aaccgcctta tcagccagat
      901 gaaatctacg atgcgctgaa acaaggtgat gtgctggtgc gcctgggtgg tctgcgcgta
      961 ttgcgcattg gcgacgacgt gtatgccaat ggtgagaaga tcgattcccc gcaccgtccg
     1021 gcactggatg cactcgccag caacattgcg ctgactgcgg agaattttgg cgatgcgctg
     1081 gaagatccgt cattcctcgc gatgctcgcg gcgctggtca atagcgggta ttggttcttc
     1141 gaagggtaag tttggattta agccggatgc agcatcacat gccgcatccc ttttcgctta
     1201 cttccgttgc gccgttaact cggcaatacg gacgatcacc tgcaccgctt tttccatacc
     1261 ttccagaatc acaaactcat gcttaccatg atagttgtaa ccgccagtga acaggttcgg
     1321 gcaaggtaat cccataaacg acaactgcgc gccgtcggta ccgccgcgga tcggtttcag
     1381 ttccggttca atatcgcagt cacgcatcgc ctgctgggcg atatcgagaa tatgcggatg
     1441 ctcaaccact ttctcgcgca tattgtagta actgtcttca atcaccaatt caatgtagca
     1501 atcaggatgt aaccctttgc ccactttttt ggcgatctcc atcattttac gtttacgcgc
     1561 ttcaaactgt ttacggtcga aatcacggat gatgtagtgc atatcggccc gttcaacggt
     1621 gcctttcatg ctcgccaggt gatagaaacc ttcatagcct tctgtcattt ccgggctttc
     1681 atccgccgga acttccgcat gaatacgtgc cgccagcgac agcgcattta ccatcactcc
     1741 tttcgccgtg cccggatgaa cattgttacc gacaattttt atattgaccg atgcggcgtt
     1801 gaagttttca aactccagtt cgcctacgcc accaccgtca acagtgtaag cccagcgggc
     1861 atcgaaggca tcaacatcaa aatgtttcgc ccctttgccc acttcttcat ccggggtaaa
     1921 ggcgacgcga atatcaccat gcggaatgtt tttctgttgc aataccgcca gcgcggtcat
     1981 gatttctgca atacctgctt tgtcatcagc acctaacaag gttttaccat cggtggtaat
     2041 caacgtctga cccagtagct gatgcagcac cgggaacata accggtgata aaacttcatc
     2101 gccgataccc agcgcaatat cgccaccgcg atagttttca acaatttgcg gattaacatt
     2161 tttgccgctg caatccggtg aggtatccac atgagaaata aagccaatcg ccgggatatc
     2221 gccagggacg ttagccggta acgtcgccat caaagtgccc ttctcactta aggtcacatt
     2281 gataagcccc atttcttcga gctgctcttt cagcagatgc aataacttcc attggccttc
     2341 cgtgctggga acctgtctca cccctgcttt tgattgggta tccagagaca cgtagttcaa
     2401 aaatcgctca agtagtttat ccatgtagtc accctcactt tttgtgacaa cattattaag
     2461 aagcaagaaa agacaaatat tgcgtcaggt cacttttacc cctgcaagcg ggaatattta
     2521 tcagcattac cttaatgaat ataaagctaa gccagtaatt cacaacaaag attggcgatt
     2581 caagcggttt gccgtagaat taccgccctc attaagagtc accaaggtgg ttaaccacaa
     2641 accccgcatc ggtaagccat ccgttgcgtt tacatgggac agagtaaaaa attgaataaa
     2701 caaccgagtt cgctttcacc gctggtgcaa ttggcgggaa ttcgcaaatg ctttgatggt
     2761 aaagaggtca ttccccagct ggatctgact atcaacaatg gcgagttcct cacgctgctt
     2821 ggcccttctg gctgcggtaa aacaaccgtt cttcgcctga ttgcaggtct ggaaactgtt
     2881 gattccggac gcatcatgct ggataacgag gacatcaccc acgttccggc ggaaaaccgc
     2941 tatgtgaaca ctgttttcca aagctatgca cttttccccc acatgaccgt gttcgaaaat
     3001 gtggcctttg ggttgcgcat gcaaaaaacc cccgctgctg aaattacgcc ccgcgtgatg
     3061 gaagccctgc ggatggtgca gctggaaacc ttcgctcaac gtaaaccgca tcagctctct
     3121 ggtggtcaac agcaacgcgt cgccattgct cgcgcggtgg ttaacaagcc tcgtctgttg
     3181 ttgctggatg agtcgctctc agcgctggat tacaaactgc gtaagcaaat gcagaacgag
     3241 ctgaaagcgt tacagcgtaa gcttggcatt acattcgttt tcgtgactca cgaccaggaa
     3301 gaagcactca ccatgtcgga caggattgtg gtgatgcgcg atggtcgcat tgagcaagac
     3361 ggcacgccgc gtgaaatcta cgaagagccg aaaaacctgt ttgttgccgg gttcattggc
     3421 gaaattaata tgtttaacgc cacggtgatc gagcgactcg acgagcagcg cgtacgcgcc
     3481 aacgttgaag gccgcgaatg taatatctac gttaacttcg ccgttgaacc ggggcaaaaa
     3541 ctgcatgttc tgctgcgccc ggaagactta cgtgttgaag agattaacga cgacaaccac
     3601 gctgaagggc tgattggtta cgttcgtgag cgtaactaca aaggcatgac gctggagtcg
     3661 gttgttgaac tggaaaatgg caagatggtg atggtcagcg aattcttcaa tgaagacgat
     3721 cctgactttg accactctct cgaccaaaaa atggccatta attgggtaga aagctgggag
     3781 gtcgtactgg ctgatgaaga acacaagtaa gttccagaat gtagtgattg tcactattgt
     3841 cggttggctt gtgttgtttg tctttctgcc caacctgatg atcattggca ccagcttttt
     3901 gacccgcgac gacgccagtt tcgtcaaaat ggtctttacg ctggataact acacgcgtct
     3961 gctcgatccg ctctattttg aagtgctatt gcactcgctg aatatggcgc tgatcgccac
     4021 cctcgcctgc ctggtgctgg gctatccatt tgcctggttt ctggcgaagc tgccacacaa
     4081 ggtgcgtccg ctgctgctgt ttctgctgat tgttccgttc tggaccaact cattgattcg
     4141 tatctacggg ctgaaaattt tcctcagcac caaaggctat ctcaacgagt ttttgctctg
     4201 gctgggcgtt atcgacacac caatccgtat catgttcacg cccagtgcgg tgattatcgg
     4261 tctggtttac attctgctgc cgtttatggt gatgccgctg tactccagta tcgaaaaact
     4321 ggataagccg ttgcttgagg cggcgcgcga tctcggtgcc agcaagttac agacttttat
     4381 ccgcatcatt attccactga cgatgccggg gattattgcc ggatgtctgc tggtgatgct
     4441 gccagcgatg ggcctgttct atgtgtccga cctgatgggc ggtgcgaaaa acctgctgat
     4501 cggtaacgtc atcaaggtac agttcctcaa tattcgtgac tggccgtttg gtgcagccac
     4561 cagcattacg ctgactatcg taatgggcct gatgttgctg gtttactggc gcgcttctcg
     4621 tttgctgaat aagaaggtgg aactcgaatg atcggtcgac tgcttcgcgg cggttttatg
     4681 accgctatct acgcgtacct gtatatccca atcattattt tgattgtgaa ctcctttaac
     4741 agctcgcgct ttggcatcaa ctggcagggt tttaccacca aatggtatag cctgctgatg
     4801 aacaacgaca gcctgttaca ggcagcgcag cattcactaa caatggcggt gttttcggcg
     4861 acgtttgcta cgcttatcgg ttcactgacg gcagttgcgc tgtaccgtta tcgctttcgc
     4921 ggtaagccgt tcgttagcgg aatgctgttt gtggtaatga tgtcaccaga tatcgtgatg
     4981 gcaatttcgc tgctggtgct gtttatgctg ctgggtattc agcttggctt ctggtcgctg
     5041 ctgttctcgc atatcacctt ctgcctgcca tttgtggtgg tgacggtgta ttcgcgcctg
     5101 aaaggttttg acgtgcggat gctggaagcg gcgaaagatc tcggtgccag cgaatttacc
     5161 attctgcgaa aaatcattct gccactggca atgccagcgg tggcggcggg ctgggtgtta
     5221 agctttaccc tgtcgatgga cgatgtggta gtttcttcat tcgtcaccgg accaagttat
     5281 gaaattctgc cgttaaaaat ttattcgatg gtcaaagtcg gcgtatcgcc ggaagttaac
     5341 gcgctggcaa ccatattact ggtgctgtcg ctggtgatgg ttattgccag ccagcttatt
     5401 gctcgtgata aaacgaaagg taacacaggg gacgttaaat gaaaaaatgg tcacgccacc
     5461 tgctcgcggc gggtgctctg gcactgggca tgagcgccgc tcacgccgat gacaacaaca
     5521 cgctgtattt ctacaactgg accgagtacg tgccgccagg actgcttgaa cagttcacca
     5581 aagaaaccgg tattaaggtt atctattcga cttacgagtc gaacgaaacc atgtacgcca
     5641 agctgaaaac gtacaaagac ggtgcctatg acctggtggt tccttcaacc tattacgtcg
     5701 ataaaatgcg taaagagggg atgatccaga agatcgacaa gtcgaagtta acgaacttca
     5761 gcaatctcga tccagacatg ctcaacaagc cttttgaccc gaataacgac tactccattc
     5821 cgtatatctg gggtgcgacg gcgattggtg ttaacggtga tgcggtggat ccgaaatctg
     5881 tcaccagctg ggccgatctg tggaagccag agtacaaagg cagcctgctg ttgaccgacg
     5941 atgcccgtga agtgttccag atggcgctgc gtaagctggg ctactccggt aacaccaccg
     6001 atccgaaaga gattgaagct gcatataacg agctgaaaaa actgatgcca aacgtcgcag
     6061 cgtttaactc cgataacccg gctaacccgt acatggaagg cgaagttaac ctcggcatga
     6121 tctggaacgg ttctgctttc gttgcacgcc aggcgggtac gcctattgac gtggtgtggc
     6181 cgaaagaagg cggcatattc tggatggaca gcctggcgat cccggcaaat gccaaaaaca
     6241 aagaaggcgc gctgaaattg atcaacttcc tgctgcgccc ggatgtggca aaacaggttg
     6301 ctgaaactat cggttatcca acgccaaacc ttgcggcgcg taagctgtta agtccagaag
     6361 tggcaaacga taagacgctc tacccggatg ctgaaaccat taaaaatggc gaatggcaga
     6421 atgacgttgg cgcagccagc agcatttatg aagagtatta tcagaagctg aaagcaggac
     6481 gttaattcag caagcgggcg gtggtattac cgcccgtttt ttagttagca aggatgtggc
     6541 taatgagtca ggacagtaaa gttttatccc gctctttcgt cggtattgtg gtccgcttgt
     6601 aggcctgata agcgtagcgc atcaggcaat gcttcctgct ttataatccc tccagcaact
     6661 tttcaacaaa ttctggcacc acctggcttg ccgggccgta atatttctcg gcaaattcat
     6721 taccaacctg actcggttca agattcagtt caacggtgtg agcaccatgt agtttggctt
     6781 cgtgtacaaa cccagccgcc ggatagacat gcccggaagt accaatggca ataaagacat
     6841 ccgccatcga caacgccata taaatttcat ccatgccgag tggcatttcg ccaaaccaaa
     6901 cgacgtgcgg gcgcaagggt gccgggaact ggcaacaatg gcatttatct tctggcgtaa
     6961 cgtctccggt ccagtcgaga acctgaccac tttgcgaaca acgaactttc agcaattccc
     7021 catgcatatg aatgacattg gtattacctg cgcgttcatg caggttgtct atattctgcg
     7081 ttaccagcaa aaagcgatcg ccgagagcgt cttgcagttt agccagcgca agatgcgcgg
     7141 cgttaggctg aatttctggc tgctgcagct gtcgacgacg ggcgttataa aacgcttgca
     7201 ccagttcagg atcgcgatcg aaaccttccg gcgttgccac atcttcaacc cgatgttctt
     7261 cccacaggcc atctgcggcg cgaaaggtac gaatacctga ttccgcagaa attcctgccc
     7321 ctgtcagtac gagtactctt ggtttttcca ttgcttccgg caccacttta tctctgaaaa
     7381 aaatacgctg acgcaaacgc tcgcgcaggc gacgtttatt tttacgaaaa cgacttaacc
     7441 gatgaccccg acgcgacagc atagcaacct ctgttgttta atcggttaga tgtaggaagg
     7501 ccgcaccacg cattcctccc gcatcaccgt ggcgcgcgcg ttcaatgcgc ggaacacgag
     7561 ctacaggtaa gagatgacaa ggcaacctgt ccgccagttg cgttgtgatt gccgggaaat
     7621 tcgataagcc accaccaatg acgaccaggt cagggtcaac aatggtcagg atatttccca
     7681 gagaaaccgc taataaatcc agataacgct caacgtgcgc ccttgcctgc tcatcgcctt
     7741 gatcataaag cgcaataatt tcgggagcct gcaacggttg atgataatag tgttgataca
     7801 gccacgcaaa accgcgacca gacagataat tttcaatgca gccatgctga ccacagccgc
     7861 agcggcgtaa cgggaaatcc aaccccatca tggttaacgc atcaaccggc agacgcatat
     7921 ggccaaactc gccggtaatg tagcttttac cagtaatcgg tttgccgttg aaaaccagcc
     7981 cgccgccaac gccggtgccg agaattaacc ccatcaccag tggatattgc gtaaattcat
     8041 catcccaggc ttctgaaagg gcaaaacagt tggcatcgtt atcaaggcgt acatcgcgat
     8101 caagacgtgc gctcaggtcg gcacgcagcg gtttgccgct ggcagcgggg acattagcgg
     8161 catacagctt accatcttct gtttccggca ttcccggaat accgatgccg acagaacctt
     8221 tacagccaaa acgttggtca gcttcagcta ccagctcaca cactgcatct aaaaatgcgt
     8281 catagctgtc acgcggtgtc ggcacccgct tttcccactg caactgccga ccgctatcaa
     8341 acacgccaag cgcaattttt gttccaccaa tatcaaaccc gtaatacatt gccgctcctt
     8401 gttttaatgt actgccttta ctggccgcta aggactcgcg cagggtcaat attactggcg
     8461 cgccgcgccg gataccaact tgccaaaaga ctcagcaaca atgctgtgac cagtacgtag
     8521 aagacgtcca gccagtgcaa ttccgatggc aggaagtcaa taaaataaat atcgctggag
     8581 aggaactgat gaccgatcag cttttcaatc cactcaataa tcggggtaag ttgcagtgaa
     8641 acaactacgc cgataaccac cccacacagg ctgccgaaca gccctgccag caatccatac
     8701 cagacaaaga tggcgcgaat taaaccatct ttcgccccca gcgttcttaa tactgcgata
     8761 tcgccactct tgtctttcac cgccatcact aaggtggaga cgatgttgaa acaggccacg
     8821 ccaatcacca gtaccatcgc cagatacata atggcgcgga tcatctggat atcgcgatac
     8881 atatagccgt aagtaccaat ccaactttta atataaacat agctgttggt cacttcaccc
     8941 gcatcgcgta ccagcttatt ggcgttgaaa acatccgtca ttttaagggc aatacctgac
     9001 acgctggaac ccatatcaag atattgttgg gcatccgcca gcgggatcat ggcaaaactg
     9061 tgatcgagtt gaccactcaa ctgcaaaata ccggcaatgt gcaaacgcac acgttttggc
     9121 tgcatcagtt tatgctcggg attcgagttg gggatcatga tcgacaccca atcgccctgc
     9181 ttcactttca gcgcatccgc cacgcctttg ccgatgataa tttgctgttc gcccgcttta
     9241 aaattgcgcc acgcatcccc ctgaacaaac gagggtaatg cgctcagacg ctgttcctgt
     9301 tgtgggttaa cgcccttcac ctggattgcg cgcagattcg ctccactttc caccagaccg
     9361 gtgaaattga tatacggcgc agcggcggca atacccggca ctttctgcac gttatccagt
     9421 gcttcctgcc agttagtcca tggctgattt accgcttcga tttcgccatg cggcaccacc
     9481 gccagaatgc ggttattcag ttcgcgttca aagccgttca tcgcgcttaa gccgacgatc
     9541 aataccgcca cgccgagggc aatgccaatg gtagaaatga cagagattag cgacaccatg
     9601 ccgccgcgtc gccgtccgcg actaaaacgc aggccaatca ataacgataa aggcatcgcc
     9661 attactccgc ccccatcagg ctcagttccg ccgtcagacg tccatcacgc atttccagtt
     9721 ggcggctcat acgtttcgcc agttgcaggt cgtgagtgac caccaggaaa gcggtgccct
     9781 gcaagcgatt caactcccca agcaactgga agatgctgtc ggcgttacgc gcatcgaggt
     9841 taccggtagg ttcatccgcc agtaccagac gcggattatt gaccagcgca cgggcaatcg
     9901 ctacacgctg gcgttcgccg ccagaaagtt cagatgggcg gtgattcgca cgatgctcca
     9961 gccccaccgc ttttaacatc tccagtgcac agctgttgat ttcagcgggc tttttcttgc
    10021 caatcagcag tggcatagcc acgttttcca gggcagtaaa atccggcagc aggtggtgaa
    10081 actgataaat aaagcccagc ttctggttgc gcagttctgc tttcgccgct gaagacagtt
    10141 tgctcatcgg ctgaccgtta aagatcacat cgccggaggt tggcgtatcc agcccgccta
    10201 acaggtgcag caaggtactt ttaccggaac cagagctacc gacaattgcc atcatttcac
    10261 cttcgcccac gctgaaactg acattgagca gcacatcggt ttgcacactg ccttcctgat
    10321 agcgtttgca caggttgtcg cattgcaaca ggatcttatt cataacgtaa agcctcagcg
    10381 ggttgtgtgg cggcagcgcg ccatgaaggg taaagcgtag acagcagcgc gatagccatc
    10441 gccaccagcg caataacaat gacctgtaaa ggttcgatag ccaccggcag cgccgcgcca
    10501 tcaagcagga cgccgattat cggcatcaga ttatttaact ggctggcaag cagtgcgcca
    10561 agcgccgctc cgaggattgc accgataatc ccggcgctag ccccttgcac cataaagacc
    10621 atcatgattt gtcgcggagt taagccttgc gtttgcagga tcgcgacttc gccctgcttc
    10681 tccatcacca tcagccccag cgaggtaata atgttaaacg ccgcaacagc gacaatcagg
    10741 ctcagcagta agcccatcat atttttttcc atgcgtacgg cctggaacag ctcgccttta
    10801 cggtcacgcc agtcctgcca tttgctgcct tcaggcagtt tttgctgact taaagagtca
    10861 actctcagcg gctcatccag ccacaaacgc cagccggtaa tattggctgc cggataacgc
    10921 atcagacgcg atgcatcctc aatattcacc agcatttcat agccatcgac ttcactgttg
    10981 gcggcgaaag tgccaatcac attgaacagg cgctgacttg gaatacgccc catcggcgtg
    11041 aactggctgg cagatggcac catcacgcgg atttgatcac cgcgattgac gcccaactgt
    11101 gaggcaagtt gttcgccgag gatgacatta tatttccccg gctcgaggtc agtttgtttc
    11161 acattgacca gatacggcgt tagtggatct ttttgcgccg ggtcgatacc gagcatcacc
    11221 ccgaccgcca cgctgcgcgc gctttgcagt accacatcac cggtagtaat aggtgcgacg
    11281 cgattaacgc cgtccagttt gactgccgtt tccgggagtt gctgcgggtt aagagagcca
    11341 tgctcagaag agagaattgc ctgtggcatc aggccaagga tgttgttttg cagctcgcgc
    11401 tcaaagccgt tcatcactga caatactgtg accagcgcca tcaccccgag ggtaatgccg
    11461 atggtagaaa gccaggagac gaaacgaccg aagcgatcca ctgcacgccc acgcatgtaa
    11521 cgcaggccaa taaatagagc gacaggttgg tacatgaaat ccgtctggtt gctgtagcaa
    11581 agacacgagt atataagcga aagcaagatg ggtaaacgtc tgaactgtaa gtgatcgtca
    11641 gggctaatct gaaaagcccc cgtaaatcaa tcggctaaat tgccatgttt tatcatcaat
    11701 cttgccgcct gatcttaatc tgaaaaatca tccaatacag acagataccc attacacctc
    11761 gggtcataac cagcaggatc aaccgcatgt aaattaaaaa agagcgtgtt gttctgatga
    11821 aacaaaaaga gctatggatt aaccagatca aagggttatg tatttgtctg gtggtgattt
    11881 atcactcggt cattaccttt tatccgcata tgaccacttt tcagcatccg ttatcggaag
    11941 tcctgagcaa atgctggatc tatttcaatc tttaccttgc cccctttcgt atgccggttt
    12001 ttttctttat atccggctat ttgattcgcc gctatatcga cagcgtaccg tggggaaatt
    12061 gtctcgataa acggatctgg aacatcttct gggtgctggc gctttggggc gtggtgcagt
    12121 ggctggcgtt aagtgcgctg aaccagtggc tggcacctga gcgcgattta agcaatgcct
    12181 ccaatgccgc ttatgccgat tccaccggtg agttcctgca cgggatgatc accgccagca
    12241 ccagcttgtg gtatctgtat gcgttaattg tctatttcgt ggtatgtaaa atttttagcc
    12301 gtctggcgct gccactattc gccttgtttg tactgctgag tgtggcggtt aatttcgttc
    12361 ccacgccgtg gtggggaatg aacagtgtga tacgcaattt gccttattac agccttggcg
    12421 catggtttgg cgcaacaata atgacctgtg ttaaagaggt gccgttgcgc cgccatctgc
    12481 tgatggcttc tttgcttacc gttctggcgg tcggtgcctg gttgtttact atctcgctgc
    12541 tgttgtcgct ggtatcgatt gtggtgatca tgaagctgtt ttatcagtac gagcaacgat
    12601 tcggtatgcg ctccaccagc ctgctgaatg tgattggctc caacaccatt gctatctaca
    12661 ccacccatcg cattctggtt gaaatattca gcttaactct gcttgcgcaa atgaacgcag
    12721 cacgctggtc gccgcaagtc gaactgacac tcctgctggt ttatcccttt gtcagtttgt
    12781 tcatctgtac tgttgcgggc ttgctggtaa gaaaactttc acagcgtgca ttcagcgatc
    12841 tgttgttctc cccgccttca ctgcccgctg ccgtcagtta ctctcgttaa accatcaccc
    12901 cagccggggc gattcaattt gctaaaccat gtcgattacg gataatgaac agcattgctt
    12961 atcagagata tgcccccata tgttgaggca tatcctaacg agaatctgac aaccgttatg
    13021 cctgaacaat atcgttatac gctgcccgtc aaagcgggtg agcagcgtct gctgggcgag
    13081 ttaaccggcg cagcctgtgc aacgctggta gcggaaattg ccgaacgtca cgccggtccg
    13141 gtggtactca ttgcaccaga tatgcaaaat gctctgcgtt tgcatgatga aatcagccag
    13201 ttcaccgatc agatggtgat gaatctggcg gactgggaaa ctcttcccta cgacagtttt
    13261 tcgcctcatc aggacattat ctcctcgcgc ctttccaccc tttaccagct accgacgatg
    13321 cagcgtggca tactgattgt tccggtgaat acgcttatgc agcgcgtttg cccacacagt
    13381 tttctccacg gtcatgcgct ggtgatgaaa aaaggtcagc gcctgtcacg agatgcatta
    13441 cgaacccaac tggacagcgc cggttatcgc catgttgacc aggtgatgga gcacggcgaa
    13501 tacgccacgc gcggcgcgtt gctggatctc ttcccgatgg ggagtgagct gccttatcgt
    13561 cttgatttct ttgatgatga aatcgacagc ctgcgggtgt ttgacgtcga cagccagcgc
    13621 acgctggagg aagtagaagc gatcaatctg ctgcccgcgc acgaatttcc gaccgataaa
    13681 gcggcaattg aactgttccg cagccagtgg cgcgatacct tcgaagtgaa gcgcgatccg
    13741 gaacatattt accagcaagt gagtaaaggc acattacctg ctgggatcga gtactggcag
    13801 ccattgttct tcagcgaacc actgccgccg ctgttcagtt atttccctgc caataccttg
    13861 ctcgtgaata ctggcgatct ggaaaccagt gccgaacgtt tccaagctga cacgctggcg
    13921 cgttttgaga atcgcggcgt cgatccgatg cgcccgctgt tgccaccaca atcgctctgg
    13981 ctgcgggtgg acgagctctt ctcagagctg aaaaactggc cccgagtgca gctaaaaact
    14041 gaacatttac cgacaaaagc cgcgaatgcc aatttaggtt tccagaaact gccagacctg
    14101 gccgttcagg cgcaacaaaa agcgccgctg gatgcgctgc gtaagttcct cgagactttc
    14161 gacggtccgg tggtgttctc ggtagaaagt gaaggtcgcc gtgaagcgct gggtgaactg
    14221 ctcgcacgaa ttaaaattgc tccgcaacgc attatgcgtc ttgatgaagc cagcgaccgt
    14281 gggcgttatc tgatgattgg cgctgccgaa catggttttg tcgataaggc gcgtaatctg
    14341 gcgctgatct gcgaaagcga tctgctcggt gaacgtgttg cccgccgtcg tcaggattct
    14401 cgccgcacca tcaaccccga tacactgatc cgtaaccttg cggagctgca tattggtcag
    14461 ccggtggtcc atctggagca cggcgtcggt cgctatgccg gaatgaccac gctggaagct
    14521 ggcggcatta ctggcgagta cctgatgctc acctatgcca acgacgccaa actgtatgtt
    14581 ccggtgtcgt cactgcatct gattagccgt tacgcaggtg gcgcggaaga aaacgccccg
    14641 ctgcataaac ttggcggcga tgcgtggtca cgcgcgcggc agaaagcggc ggaaaaagtg
    14701 cgtgatgtgg cggcggaatt gctggatatc tacgcgcaac gcgccgccaa agagggcttc
    14761 gcgtttaaac acgatcgtga gcagtatcag ttattctgcg acagcttccc gtttgaaacc
    14821 acgccggatc aggcgcaggc cattaatgcg gtacttagcg acatgtgtca gccgctggca
    14881 atggatcgtc tggtgtgcgg cgatgttggc tttggtaaaa cagaagtggc gatgcgcgcc
    14941 gctttcctgg cagtagataa ccacaagcag gtagcggtgt tggtgcctac cacccttctt
    15001 gcgcagcagc attacgacaa cttccgcgac cgtttcgcca actggccggt acgtatcgaa
    15061 atgatctccc gtttccgcag cgccaaagag cagacgcaaa tccttgcgga agtggcggaa
    15121 gggaaaatcg atattctgat cggtacgcac aaactgctgc aaagtgacgt caagtttaaa
    15181 gatttaggcc tgctgattgt cgatgaagaa caccgcttcg gggtgcgtca taaagagcgc
    15241 attaaagcga tgcgcgcgaa cgtggatatt ctgacgctta ctgcaacgcc gatcccacgt
    15301 acgctgaata tggcaatgag cggaatgcgt gacctgtcga ttatcgccac gccgcccgcc
    15361 cgtcgtctgg cagttaaaac ctttgtccgt gagtatgaca gcctggtggt ccgggaggcg
    15421 atcctgcgtg aaattttgcg cggaggacag gtttattatc tctacaatga tgtggaaaac
    15481 attcagaaag ccgccgaacg gctggcagaa ctggtgcctg aagcacggat tgccatcggt
    15541 cacgggcaga tgcgcgagcg cgaactggaa cgggtgatga atgatttcca tcatcaacgt
    15601 ttcaacgtgc tggtttgtac caccattatc gaaaccggga tcgacatccc gacagccaac
    15661 accattatca ttgaacgtgc ggatcacttc ggtctggcgc agctgcacca gttacgcggt
    15721 cgcgtcggac gttcacatca tcaggcatat gcatggctgc tgacgccgca tccaaaagcg
    15781 atgactaccg atgcacaaaa acgtcttgaa gcgattgcct cgctggaaga tctcggggca
    15841 ggttttgcgc tggcaacgca cgatctggag attcgcggcg cgggtgaact gcttggcgaa
    15901 gaacaaagcg gttcaatgga aaccatcggt ttctcgctgt atatggagtt gctggaaaac
    15961 gccgtcgatg cactgaaagc cggacgcgag ccgtcgctgg aagatctcac cagccagcaa
    16021 acagaagtcg agctgcggat gccgtcgcta ttgccagatg atttcatccc tgacgtgaac
    16081 acgcgtctgt cgttctacaa acgtattgcc agcgccaaaa cggaaaacga actggaagag
    16141 atcaaagtcg agcttatcga tcgcttcggc ctgctgccag atccggcgcg taccctgctg
    16201 gatattgccc gtctgcgcca gcaagcgcag aaactgggga tcaggaagct ggaaggtaat
    16261 gagaaaggcg gggtgatcga atttgccgag aagaatcacg ttaatccggc ctggttgatt
    16321 ggtttgctgc aaaaacagcc gcagcattac cgccttgatg gtccgacgcg cctgaaattt
    16381 attcaggatt tgagtgagcg gaaaacgcgt atcgaatggg tacgccagtt tatgcgtgaa
    16441 ctggaagaga acgcgatcgc ttaatgtaaa tcgggggcgt ttctgcgccc ccatctgcaa
    16501 catttacaaa ttctttgcac tttcctgcac tatccgacac tgtcaccatc cataattcag
    16561 gcttatctgt ttattacaat aaccttatat ttattatgta tttttggtga tgatcaaaac
    16621 gcgtttttct cgctggctaa cgttttttac gttcgccgct gccgtggcgc tggcgctacc
    16681 ggcaaaagcc aacacctggc cgctgccgcc agcgagcagt cgtctggttg gcgaaaacaa
    16741 atttcatgtg gtggaaaatg acggtggttc tctggaagcc atcgccaaaa aatataacgt
    16801 cggctttctc gctctgttac aagctaaccc cggcgttgat ccttacgtac cgcgcgcggg
    16861 cagcgtgtta acgatcccgt tgcaaaccct acttccagac gcgccgcgcg aaggcattgt
    16921 gatcaacatt gcggagctgc gtctctatta ctacccgccg ggtaaaaatt cggtaaccgt
    16981 gtatccaata ggtattggtc agttaggtgg tgacacgctg acaccgacaa tggtgaccac
    17041 cgtttcagac aaacgtgcaa acccaacctg gacgccaacg gcaaacatcc gcgcccgtta
    17101 taaagcacag ggaattgagt tgcctgcggt agtgccggct ggaccggata acccaatggg
    17161 ccatcatgcg attcgtctgg cggcctatgg cggcgtttat ttgcttcacg gtacgaacgc
    17221 cgatttcggc attggcatgc gggtaagttc tggctgtatt cgtctgcggg atgacgatat
    17281 caaaacactc tttagccagg tcaccccagg caccaaagtg aatatcatca acactccgat
    17341 aaaagtctct gccgaaccaa acggtgcgcg tctggttgaa gtacatcagc cgctgtcaga
    17401 gaagattgat gacgatccgc agctgctgcc aattacgctg aatagcgcaa tgcaatcatt
    17461 taaagatgca gcacaaactg acgctgaagt gatgcaacat gtgatggatg tccgttccgg
    17521 gatgccggtg gatgtccgcc gtcatcaagt gagcccacaa actctgtaaa tcctggataa
    17581 aaaaaatccc cgcgacacgg catgtcgcgg ggaaaaaatc agcagtagca ggcattaatg
    17641 agggttaatg cttatttata aattactgct gttccatgga gggtattcgg accggttaca
    17701 gaagtaatac ggaaagattt tgcgcccatc tcatccgctt tttgcgccag ctgctcttcc
    17761 agcgatccca gatttgtccc cgcgttagca ctgatagtac cgactttttg ttggccttct
    17821 ggcgttgact gaacttcgac agccgcaaag ttggcaaatg acatggagct taaaatcgcc
    17881 gcagcgatga ggtttttacg tttttcataa tagtggcctt atgcagatga atgacgtcgc
    17941 aagaaattac ttaacgatcg ataactaaat gatagatgtg atctggatca catacaagat
    18001 attttttata acaatcatta attaatttaa aaatctttaa atttcagtca aatagaaagc
    18061 aattattttt atgggatctg gactggtgaa cggaggtgat tatttttata ataacctttc
    18121 attcacagtc accaggtaca acggcatcat ggcaactgac tcaacacaat gtgtaaaaaa
    18181 aagccgtggc cgcccaaaag tgttcgacag ggatgccgcg cttgataagg ccatgaaatt
    18241 gttctggcaa cacggttatg aagcgacttc tcttgcggac ctcgtcgaag caaccggagc
    18301 caaagcgccc acgctatacg cggaatttac caacaaagag gggttatttc gtgccgttct
    18361 cgaccgctat atcgatcgtt ttgccgctaa gcatgaagca cagctgtttt gtgaagagaa
    18421 aagcgtggag tctgcgctgg ctgactattt tgcggccatc gccaactgct ttaccagcaa
    18481 agacaccccg gcaggctgct tcatgatcaa caactgcacc accctctccc cagattcagg
    18541 agatatagcc aatacgttga aatcacgcca tgcgatgcaa gagcgcactt tgcagcagtt
    18601 tttatgtcaa cgacaagcgc gcggggaaat cccgacccac tgtgacgtga cacatctggc
    18661 agaattcctt aattgtatta ttcaggggat gtcgatcagc gcacgcgaag gtgcatcgct
    18721 ggaaaaactg atgcagattg ccggaacgac tttgcgttta tggcccgaac tggtgaaata
    18781 agtcgcaaaa aaggcccctc agcctgagcg aatgaggggc caaattgcag agtacagcct
    18841 tgttatactt tgttattcaa aatcagttgc ccgttactat ctagcgggat ctgcgtaccc
    18901 ggatcgcggt ccatgcggat tttgccctgc tgatcgccaa tcttataggt cacatcataa
    18961 ccgagcattt tttctgactt gtcatacacc gttttacaac gctgttgcgt agtcgtgtaa
    19021 gtatcgcttt cctggagaga gccctggatc tggttaccgg catatccacc acccagcgcc
    19081 cccacaacag tggcgacatc tttaccgcga ccaccaccaa actgatgccc tatcacgcca
    19141 ccagcaacag cgccgagcac cgacccggta atgcgatttt catcctgcac cggtcgacga
    19201 tgggtcactg tgacgttgcg acactcctga cgcggtgttt taaccgtttc cttgattggg
    19261 gttgcagaaa caacctgagc gtattgcggg ccccgttcaa acacgttcag actggccact
    19321 gccgctacgc ccaacgcagc tgcgacacca atcccgatac ccgccaacat tgatttattc
    19381 acgggaacac ctccttcttt gcagttatgc caatcatgca agcgaaggag aaaagcagct
    19441 atcagacaga agatgaaaag cgcgggaatg actggaaata cttaaggatt cggaggagtc
    19501 tgaaggaatg cctgatgcga cgcttaacgc gtcttatcag gcctaccgct cgtaccattt
    19561 gtccagggcg gataaggcgt ttataccaca tccgccagtg tacgtcgatt aatgcaactt
    19621 caaacgcgga cggataacgc ggttaatact ccccaccagc atcattaatc cggttttaaa
    19681 gtaaccatgc agcgcaatct gatgcattcg gtatagcgag atatatacaa agcgcgcaat
    19741 tcgtccttca atcatcattg agccgcgcgt caggttaccc atcaggctac caacggtgga
    19801 gaagttcgac agcgatacca gcgaaccatg atctttatac tgataatttt tcagcggctt
    19861 accgttcatc tgcgccagaa tgttgttcat tgcgcaagtc gccatctggt gtgcagcctg
    19921 agcacgcggc ggaacaaagc ccccttccgg acgcgggcat gacgcgcagt cgccaatagc
    19981 gtaaatgtct ggatcgcgag tggtttgcag cgtcggttcc accaccagct ggttgatacg
    20041 gttagtttca agaccaccga tatctttcag gaagtctggc gctttgatcc cggctgccca
    20101 taccatcaga tcagcctcaa tatattcgcc atctttagtg tgcaggccgc cttcatcagc
    20161 actggtgacc atggtttgcg tcagcacgcg aacgccaagt ttcgttagct cgttgtgggc
    20221 cgcagtagag atacgtggcg gtaatgcagg caaaatacgt tctcccgctt ctaccagcgt
    20281 tacgttcagg gcttcgttgg tcaggccttt gtaaccgtag ctgtgcagtt gcttgatcgc
    20341 gttgtgcaat tcagcggaga gttctacacc cgtcgcgccg ccgccgacaa tcgcaatgtt
    20401 cactttgcca tttgcgccca ggttggcaga gtatttcagg aacagattca gcatctcctg
    20461 gtggaagcga cgcgcctggt gcgggttatc gaggaaaatg cagttctctt tgacgcctgg
    20521 cgtattgaaa tcgttagagg tgctacccag cgccatgacc agggtgtcat aggcgatttt
    20581 acgttccgga accaacagtt cacctttctc gtcgcgtagt tctgcaatag tgattgtttt
    20641 cgcttcacga tcaatatcaa tgacggaacc caactggaac tggaaaccat gattgcgcgc
    20701 atgggccaga tagctcaacg catcgacgcc ttcatcaagc gagccagtcg ccacttcgtg
    20761 cagcagtggt ttccacaggt ggctgtggtt acgatcgacc agcgtaattt tggctttttt
    20821 cttacgtccc agcttatgcc ccagctgtgt tgccatttcc agcccaccag caccgccgcc
    20881 gacaatcaca atctttttca atggcgtagt caacgtgacc cccttaaatt tattaaccaa
    20941 ttgttaatta atggttatta acatagctct taattaacaa caggttacag caatgaaaat
    21001 gttcttcgct attgagaata acatgaatgg tgcattggtc ataccaaaat tgatgttaat
    21061 caagttttgt tgcttaaaag ataggtaact ttaccggggg agatcaaaaa atagagttta
    21121 ttgtttatta tttgagcaaa ttcggaaggg taatataggg tgaagcgtat atcgagggcg
    21181 tttggtcgtc ctgataagat gcgcagcatc gcatcaggca ttgatgctcg actgtcggat
    21241 gcggcgtgga cgccttatcc gacttacaga attcggagcg catcaggtac cggcaaggtt
    21301 acctgatgcg acgagggcat ttacccgagg gttttgaacg ctttaatgcg ctgtaaatgc
    21361 ggggagatat tcttgaattt gtgcgtctgc tcttcgtccc agacaatctc gtaataatga
    21421 tgcaactctt cagatgtccg ctggctgtta agtgcttcat cattacgcga caaaataacc
    21481 agacaacgat cgcgattctt ctcgcggaag ttggtcacac acttagtggc aatatcggca
    21541 tactcttccg ggcgatcaat cttgccttcc atgttctcat aagggaacaa attagggttg
    21601 aagatcacct ggcggatatc gcaaagaaaa ccaatccgtt ccgcccagta tccgcccagg
    21661 ccaacgccgc aaattagcgg acgctcgtca acgttcagtt gcaacatttt gtccacttct
    21721 ttaagcagat gctgcatatc atgtttcgga tgccgcgtac tgtagcttat caagcgtaca
    21781 tccgggtcaa taaactgcaa ttgtaagact ttctcgtggt tacccggact gttagagtca
    21841 aaaccgtgta aatagataat catcgcttcc tcacataagc cagggttagt gacctgcttt
    21901 ctcttcctgc cagcgttcat gtaactgatt cagacgggtg ctgatcgctt tccagcgagc
    21961 cgagtccatc agttcctgtc gcgaaaatga acctttatga tacaaacgtg taacacgttc
    22021 tgccttgatc ggtgacagat tatctaacac gctgacggcc cctttacgat tattgcagac
    22081 caggatcata tcgcaacccg catccagtga tgcctgcccg cgttcggcat aactgcccat
    22141 aatcgcggca ccttccatcg ataaatcgtc agagaaaatc acgccgtcaa aacccagttc
    22201 ctgacgcaaa acggttttca gccagtaggg agaaccgctc gccggacgcg gatcaacatc
    22261 actgtagatc acatgcgcag gcataatggc gtcgagttta ttttcgcgga ttaaggaact
    22321 gaagaccgac atatctttag cgcgaatctc cgcttgtgga cgtggatcgc acggtgtttc
    22381 tttgtgtgag tctgccgtta ctgcaccgtg tcctgggaag tgtttcccgg tcgttttcat
    22441 tccggcttca tgcataccat caataaaccg gctggcaatt gccagggctt tttgtggatc
    22501 ggcatgataa gaacgctcgc caatcgccgc gctgatatgc ccgacatcca gcactggcgc
    22561 aaagctgata tcaatatcca tagcgatcat ttcgctggcc atcagccaac ccgcctcttg
    22621 cgccagtttg ccgccctctt ccattcccaa cagcgcagca aaggattgtg ctgccggtaa
    22681 gcgggtaaaa ccttcgcgaa aacgctgcac gcgtccacct tcctgatcta ccgccaccac
    22741 cagatgattg cgcgatgctg cgcggatctg gcgcaccagt tcacgtaact gggcaggatc
    22801 atgatagtta cgcgtaaaga gaatcagccc tcccaccagc ggatgcgcca gtatttcacg
    22861 ctcttccgcg tccagttcgt aaccttcgac atccaacatt actggaccca cactgctctc
    22921 cttattgttt tattaatagc tgccgccagg tgtcatcggc cagcctgata aattgttgat
    22981 cgccggtttg tcgccagcgg tactcaaacc accctccttt gagcatcagc agccagggaa
    23041 accatcgcct gacctgacgc cataattgcg ccggataaat cttcgcgcga gtggcatagt
    23101 cattgaccaa ttgccggtgc tgttcagtat tttccaccca caccgccgcc agttccagcg
    23161 cgatatcacc atctccagca tactcccagt cgatgagttt taaccctgat gcgctatgca
    23221 ctaaatttcc ggcgtggaca tccatatgca atggacttaa gcgtaaaggt cgtggttccc
    23281 gcgctttgcg cagacgtttt aacattcgca gccaacccac agtccgccgc gccggatcgc
    23341 tttgctgcca gtacagttcc agtaagggca acagcgttat tcgccagcca aaacgtggtt
    23401 gttgatgtag ataatacagc aagcctgcca gttcgttggt atccggcaaa tacgttttta
    23461 ccgcgccggg cagatagtcg actaccatcc agtcacggag atataaatgc ggcttcggtg
    23521 caatgcttgc gggtagttgt gataaagccc gatactggcg caagaacgcg gactgcggcg
    23581 catcaggatc gtgcggctga cgcacaacaa aacgctggcc ctgatgttca atgagaaaac
    23641 taccgccact aagcccacta ttaaacgtcg tgacgggatg aaactgcggg aaaaagcgcg
    23701 acagcaattc gtcgcgcgtg atgggattat tgctgcgaaa cggcaccttt acctgaccag
    23761 ataatttcgc ccgtctgcac cagcatcagc tgcatttgta gggtcggagc gttaacgttg
    23821 ccagaggcgc tggagtacag cacgtaatga gcgccgacat tgcgggcaat gcctatggct
    23881 ttactacggg tgcctaaact gtcctgcggc gacaaaccta actgttgctt agccatcgac
    23941 agttgctggg cggaaaccag ggtaaatttc ccgttattag ccagcgcatt tcgcagcgtt
    24001 tcggtcgctt ctgcggcatt cagcgaaccg ttagtacggt tgttaacgct atcaaccagc
    24061 aggacgctac ccgcagtcac cccgtcagcc ccaagcatct tactgaccat cggctgcatt
    24121 gcgccattcc agtcataatg gcgaatatgc ggcgcaggcg gtgccgttcg atcttcgtgc
    24181 tcaattgggc ctggctgctg cgggatcgtc ggcaccgagg gcactgtggg gacaggctgt
    24241 tgtggctcgg ctggttgttc cggcgctggt ttcacttctt ctaccggtgc aggttcacgt
    24301 tgccccacac acccggcgag aaacatcgcc agcgcggtaa tcaaggcgta gcgactcatt
    24361 tttgtcatca agattcaccc ctttacaaat atagataaag tctgactttg tgcgccccca
    24421 gaaaattggc gctgccgtac agcgttaccg ccgaatgtgc gggaatggta acgctgcgtg
    24481 gcctttccag aggatgcatc tccagccctc tggcgtcata ccagtaaaaa cgataatgaa
    24541 cggtaacggg ttcttgcctt tcgttataaa gcgttgagga tgctgaaggt tgaatatcag
    24601 acgtcgaaag gacgggcttt tctgcactga tgcccgcagc caataaagat gactccatca
    24661 ccagcgattg ctcatcattc accggaattt ccggatgtga acgacagccc accagcagta
    24721 acaacaccag agacaccagc ccaaagcatc cttttctcat cgttacagac ctttatgcgc
    24781 cagcattggt cccagcggac ggccacccaa caagtgcata tggatgtggt aaacctcttg
    24841 tccgccatgg cggttggtat tcatgatcag acgatagcca tcttcggcaa taccttcttg
    24901 ctcagcaatt tttgccgcta cggtgatcat gcgtcccagc gcctgctcat gctcagctga
    24961 cacgtcgttc acagtcggaa tgagaatatt cggaatgatc agaatatgcg ttggcgcttg
    25021 cggcgaaata tcgcgaaacg ccgttaccag atcatcctgg tagacgatat cggaggggat
    25081 ctcacgacga ataattttgc tgaatatagt ttcttctgcc acgacatttt cctttttcaa
    25141 atagccatgc gttgcgccat gctacgccgc gccacactgc gagtatagag tatggtcgag
    25201 ttaaacctgc tctttcaatc ttacctcacg atttcttaag caaaaaatcg ttttgctgcc
    25261 gtatatatcg ccattattcc catttctgcc tgcgaatgcg ctgaaaagac attctctgtt
    25321 tggtttacac gacaaatatg aatgcgtata tttctcattt gcatttgcat ttgcatttgc
    25381 agcaaaatta ttcgcacact aaatataacc gctcatcacc ctcctccggc atgagcctga
    25441 cgacaacata aaccaagaga tttcaaatgc tttcaacaca atttaacagg gataatcaat
    25501 atcaagtcat caccaaaccg tcactacttg ccggttgcat agcactggca ctattacctt
    25561 ctgccgcttt tgctgcacca gccactgaag aaacggtgat tgttgagggt tcagccacag
    25621 ctccagatga tggcgaaaat gattacagcg taacgtctac ctctgcgggt accaaaatgc
    25681 agatgactca acgtgatatt cctcagtcgg tcactattgt tagccagcag cggatggaag
    25741 atcagcagtt acaaacgctg ggcgaagtga tggaaaacac gctggggatc agcaaaagtc
    25801 aggcggattc cgatcgtgct ctttattatt cccgcggatt ccagatcgat aactatatgg
    25861 ttgatggtat ccccacctat tttgaatcgc gctggaatct gggcgacgca ctttctgata
    25921 tggcactgtt tgaacgcgta gaagtagtgc gtggcgcgac aggactcatg accgggacgg
    25981 gtaatccatc tgcggcaatt aatatggttc gaaaacacgc gacctgtcgt gaatttaaag
    26041 gcgatgtctc ggcggaatac ggtagctgga acaaagaacg gtatgtggcg gatttacaaa
    26101 gcccactcac cgaagacggt aaaatccgcg cgcgaattgt cggcggctac cagaataacg
    26161 actcatggct ggaccgctac aatagtgaaa agaccttctt ctcgggcatt gtcgatgctg
    26221 atttaggcga tcttacgacg ctttcagccg gttacgaata tcagcgcatt gatgttaata
    26281 gcccaacctg gggcggttta ccgcgctgga atactgatgg cagcagcaac agttacgatc
    26341 gcgcacgcag taccgcacct gactgggcgt acaacgataa agagatcaac aaggtcttta
    26401 tgaccctgaa gcagcggttt gctgatacct ggcaagcgac actgaatgcc acccactctg
    26461 aagtcgaatt tgacagcaaa atgatgtatg tcgatgccta tgtaaacaaa gcggatggta
    26521 tgctggttgg gccatacagt aattatggac ctggctttga tcatgtcggc ggcaccggtt
    26581 ggaacagtgg caaacgtaaa gttgatgcgc tggatttgtt cgctgacggt agttatgaat
    26641 tgtttggtcg tcagcacaat ctaatgtttg gtggcagtta cagcaaacaa aacaatcgtt
    26701 cagttcatgg gccaacatct tcccggatga aattggcagt ttctacaact ttaatggcaa
    26761 tttcccacaa gccgactggt catcacagag cctggcgcag gacgatacca cacatatgaa
    26821 atcgttatat gctgccactc gtgtcaccct tgccgatccg ctgcatctga tcctcggcgc
    26881 acgttatacc aactggcggg ttgatacgct gacttacagc atggagaaaa accacaccac
    26941 gccttacgct ggtctggtgt ttgacatcaa tgacaactgg tcgacctacg ccagctatac
    27001 ctctattttc cagccgcaaa atgatcgtga cagttcaggc aaatatctgg ctccaatcac
    27061 cggtaacaac tacgagctgg gtctgaaatc ggactggatg aatagccgtc tgaccaccac
    27121 gttagccatc ttccgtattg agcaggataa tgtcgctcag tccaccggta cacctatccc
    27181 cggcagcaac ggcgaaaccg cctataaagc ggtggatggg acagtcagta aaggggtgga
    27241 atttgaactc aacggcgcaa ttaccgacaa ctggcagctg acatttggcg caacgcgcta
    27301 tcttgcagag gataacgaag gaaacgccgt taatcctaat ctgccacgca ccacggttaa
    27361 aatgttcacc agctatcggt tgcctgtcat gccagagttg acagtcggcg gtggtgttaa
    27421 ctggcaaaat cgcgtgtata ccgacaccgt gacaccgtat ggcaccttcc gcgccgagca
    27481 aggtagctac gcgctggtgg atctcttcac ccgctaccag gtgacgaaaa acttctcgtt
    27541 acaggggaac gtcaataacc tgttcgacaa aacctacgat accaacatgg aaggttctat
    27601 cgtctacggc gcaccgcgta atttcagcat taccggcacg tatcaattct gaataacacc
    27661 tgtaaaaaag gcagccatct ggctgcctta gtctccccaa cgtcttacgg attagtggtt
    27721 acggatgtac tcatccatct cggttttcag gttatcggat ttagtaccga aaatcgcctg
    27781 aacaccagaa ccagcaacca ctacgcccgc tgcgcccagt ttcttcaggc cagcctgatc
    27841 cactttagac acatcagcaa cgctgacgcg cagacgggta atacatgcgt cgaggttagt
    27901 aatgttttct ttaccaccaa atgcagcaac cagagccggt gccatttcgc tggtacctgt
    27961 tgcttttgca tcttcagtcg cgtcttcacg acccggcgtt ttcagatcca gtgctttaat
    28021 cagcacgcgg aagatggtgt agtaaacaat cgcataaccg ataccgacga tcggtaacag
    28081 ccacagtttg ctgctgttac cagacagaac gatgaagtcg atcagaccgt gcgagaacga
    28141 cgtaccgtca cgcatcccca gaagaataca gattgggaat gccaggcctg ccagaatcgc
    28201 gtggatgatg tacaggatcg gcgcaacgaa catgaagaag aactcgatcg gctcggtgat
    28261 accggtcagg aacgaggtca gcgccgcgga gatcataata ccgcccactt tcgcacggtt
    28321 ttctggttta gcagagtgcc agatagcaat tgcggcagct ggcagaccgt acattttgaa
    28381 caggaagcca ccagacagtt tacccgcagt cgggtcaccc gccatataac gcggaatgtc
    28441 gccgtggaaa acctgacctg ctgcgttggt gtattcacca atctgcatct ggaaaggtac
    28501 gttccagatg tggtgcagac caaacggtac caggcaacgt tcgatgaaac cgtaaatgcc
    28561 aaacgcaact accgggttct ggtaagcagc ccactgagag aaggtctgga ttgcagaacc
    28621 aatcggcggc caaatgaagg acagcacaac gccagtaaag atggcagcca ggccagaaat
    28681 gatcggcaca aagcgtttac cggcaaagaa gccaagatac tcaggcagct taatacggta
    28741 gaaacggtta aacatgtacg ctgcaatcgc accggagata atccctccga gtacgccagt
    28801 atccgccaag tgtttagagg cgatttcttc agcaggtaaa tgcagtacca gtggcgcaac
    28861 cacggccatg gttttaacca tgatgccata ggcaacaact gcggccagcg cggatacgcc
    28921 atcgttattg gtaaagccga gggcgacacc gatcgcaaaa atcagtggca tgtttgcaaa
    28981 gacggaaccg cctgcttctg ccataacatg cgatacaacg gcgggcagcc agctgaaatt
    29041 cgcggaaccg acgcccagca gaatacctgc gataggtagt acggataccg gcagcatcag
    29101 cgatttaccg accttttgca ggttagcaaa tgcattctta aacataattg agagtgctcc
    29161 tgagtatggg tgcttttttt acgttctcac gcgtggcaag gggggagagc ctcgccgtgt
    29221 acagggcatc taagcgccct ttatttatta cacagagtaa aataattcag tgccaatttg
    29281 tttgacggct atcacgtttc aacttcagat cattggagaa gttgcacatt ttgacaaaac
    29341 atatgtcaca atatttcaaa cgcggttatt caccgcccaa ttttcggcgg tgaactttac
    29401 tcgttttagc cattaattac gagctttaaa aaaactcatt caacggatgg attgaaggcg
    29461 ggaagcgtcg atgtgaaaca gacgggcgaa gttatcggtg gttacctgcg ccagttcttc
    29521 aacggcaaca cctttcaaca cagccatgta ttctgcaacg tcacgaacca tcgcaggttg
    29581 attctctttt cctcgatgcg gtaccggcgc aaggtaaggt gagtcagttt ccaccagtaa
    29641 tcgatccagg gggacataac gcgcagcatc gcgcagttgc tccgcattac ggaaggtcac
    29701 aatgccggaa aaggagatgt aaaatccgag atccagtaat ttacccgccg tttctctgtc
    29761 ctctgtaaaa cagtgtagta cgccaccgca atccgtcact ttttcttcgc gcagaattgc
    29821 cagcgtatcg gcacgggcgt cacgggtatg gacgataacc ggcttgttca gttcacgacc
    29881 aatctggata tgatggatga aggactcttg ctgacgtact ttagtttccg gtgtgtaata
    29941 ataatccagc ccggtttcac ccagcgctac aacaccctct tctgccgcca gacggcgtaa
    30001 atcttctaca tcgtagggat cattctggtt gagtggatgc acgccacaag aaaatacgac
    30061 gttgtcacgt tcgcctacca gatcccgcat atgtaaataa cccggtaatg ttgtggcgac
    30121 tgccagacaa aatttcacat cgcgtgcggc ggctttcgcc agaacgtcat ccacgtcctt
    30181 atgcaaagat tcataatcca gaccatcgag atggcagtgt gagtcgacta aaaacatgat
    30241 gtctctctta aagatgagga accggtagca caacgcccgg ttgcaggtaa tgctcaatac
    30301 gcagtaaaag atcggtgatg agaagctcgc ggttgatgcc tgtgacagac attaactgtt
    30361 cacgaatgtg gcaaacatcc cccagtatag cctgcaggcg cgagggagaa agatggtttg
    30421 ccagttcggc gaccagtccc ggcacatcaa cattggtcac ctgcgcagcg ccatgatggc
    30481 gttttagcgc atccatcagc aacgttgcca gccagtgtaa acgcgccgga gcttgttcat
    30541 gattaagggc cgccagcagc gaataccagt cgcccgatgg cacgctatat gccaacgcct
    30601 gacacaatgt ttcacgagcc tgccagttat ctccctgaaa caacgccagt gccgcgccag
    30661 gcgaaccggc gcttaagcgc aatgcggcaa gtaatgcatc ctgtgacatt gtcacttcgc
    30721 gtgaaagcca agtcacggcg tactgttctg gaggcggcgc aaggtaatgt aaccgacaac
    30781 gactacgtaa tgttgccagt aaacgttcag gctcgcgggt agccaggaaa aaccaggttt
    30841 ctgctggtgg ctcttcaagc gttttcagca atgcgttagc cgcggcgtcg gtcagtaagg
    30901 cagcatcggt tacccagaca actttcgcac cacctaggcg tgcgtgctca ttcagctttt
    30961 cggtgacctc acgtaccgca tcaatgccca gcgtattttt ccctttttcg ggagccaggg
    31021 tgtagtaatc gggatgcgtg ccagcctgca tcaactgaca tccacgacag tgaccgcaac
    31081 ttttgtggcc ctgcggttgt tggcagagta aataacggct cagggcgtag attaaagcat
    31141 catcgcccat gcccggtaac gcctgaatga gtagcgcatg gtgacctctt ccggcctgat
    31201 agctggctac cagtttttcg aaatcaggtc gtaaccatgg ataccatctc atgcgtccaa
    31261 ctccttcacc cagtgggtca cggtagtgcg gattgcatcc atcacggcct ccagcggctg
    31321 ggtggcatca atggtatgaa tacttttatc ttgtgcagcc agttccagat agcgggcgcg
    31381 ggtgcgatta aagaaatcga aagactcttg ctcaatacga tccagctcgc cgcgcgcacg
    31441 cgcgcgtttt aagccaactt ccggggtaac atcgagatag agcgttaagt ctgggcgaaa
    31501 atccccgaga acagcatcac gcagtgttgc cagcatatgt tggtcaatac cacgtcctcc
    31561 gccctgatac gcctgagtgg aaagatcgtg gcgatcgcca atcacccagg tgccgttagc
    31621 cagcgcaggt ttgatgaccg tttctaccag ttgaacgcgc gcggcataaa acatcagaac
    31681 ttcggcttta tcggtaatga cttcatcgcc taccgatttg atatccagca ccaggcttct
    31741 taacttttcg gcaagttgcg taccgccagg ttcccgagtg aaaaccatgt cgcggatacc
    31801 cagttgctcg agcgtctcaa ccaccacatt acgcgcggta gttttgcctg cgccttccag
    31861 cccctcaatg acgatatact tactgcgcat ttttttcctt aagcactttc agataatcct
    31921 gcacagactt gttatgactg gcaagattgg tattaaacgt gtgaccacct ttaccatcgg
    31981 ccacaaaata gagatacggc gtttttgccg gatgcgcagc agccttcagc gaatccgccc
    32041 ccggcgtagc tatcgcaccc ggcggcaaac cggtaatggt ataggtgtta tacgctgtcg
    32101 gcgtttccag gtctgcacga gaaagtttgc cattataacg ctctcccatc ccgtaaatca
    32161 cggttgggtc ggtctgcagg cgcataccaa tgcgtaaacg gttgataaat accgaggcaa
    32221 cctgatcgcg ttcactggca acggcggttt ctttttcgat aattgatgcc atcgtcacca
    32281 gctgattttt atctttataa ggcagaccgt ccgcacgccc ttcccaggcg ctatcgaccg
    32341 ctttcaccat tttcttgtga actcgcttga gtaacgcgac atcggtggta ttggcggtat
    32401 acatcaaggt gtctggccag aaccaacctt caatccactc cgggttttcc agttcaagtg
    32461 cctgcgctac ggtggcgtac ttatcgtcac tcagcgtgtg cttgatatac ggagcctcac
    32521 gcaattgctt gaggtagtcg ctcagacgca tcccttctac cagtcgcagc gggaactgtg
    32581 cttctttacc gctttccaac aatttcagca tctcgcgcac ggtcatctgc ggtgtaaagc
    32641 ggtaagtccc ggctttaaag tgagaaagat ccggttcgat acgcagcagc cattgaaaaa
    32701 cccgtgggcg attgatgatc ttatcagcat aaagctgttc accgagcgcc agacgtccgg
    32761 tccctggctt cagggtaaat atcgtctctt ctttgataag caatttgctg tcggcaagat
    32821 ggcgaacctt ccagacgccc acaccagcgg cgatacccag taccaccagc aataacaaga
    32881 taatcaataa cacttttttc atgactaatt cgggcgctca caaagtgggg ctaaatattc
    32941 atataacgtt gctgacgaaa aggtgacatc gccacaggca cgtacgggca tcactggcat
    33001 taacgcatta caaataacca tctcatctgc ctgcaacgcc tcctccagcg aggcttgcac
    33061 ttcgacaagc tgataagagg attgtgccag taaacggata cagaattgtc gcataatgcc
    33121 gttaacacct gcctgatcca gtcgcggcgt ataaactaca ttgcccttcc gccagaacaa
    33181 attagccgca cagcattccg taacccaccc ttcgctgtca aggaccagcg cctcatcagc
    33241 gtttgtctgc tcaagatgag agcgaatcaa tacttgctca agccgattaa gatgtttaat
    33301 acctgcaaga tgaggattgc gccccagccg caccgggctt agcgccaacg tcatcccttc
    33361 gttacgcaaa cggtcgtaat gtgcaggata agccgtaacg gagagaatcc gcgttgctgg
    33421 tccgctgttc aatgtgctgt accctcgccc gccactaccg cgactgatca cgactttcag
    33481 cacaccattt tgctgttctg ctgccagcgt tttcatctct tgttcaagct gaggccagaa
    33541 gtcacaggaa atcatcaacc gctgacaagc atcctgtagt cgcaggatat gcgccgataa
    33601 caaactgact ttaccgtcga taactctggc ggtggtaaaa caaccatcac caaactgcgt
    33661 tgcccgatcg cttactgcca gcgattcctg cttataaccg ttaattaaga acataccggc
    33721 tccttatcag agaattgtta gtgtggcagc atgttcacta cggaacaagt cggaataaaa
    33781 gctaagaaaa aaggcccgca agcggacctt ttatatgggt ggaaaatgac aacttagatc
    33841 tttttaaaga tcaaagaacc attagtgcca ccgaagccga aggagttaca cagcgtgtat
    33901 tccattccgc tgacctgacg tgcttcatgc ggtacgaaat ccagatcgca accttcatcc
    33961 gggttatcca ggttgatggt tggcggaaca gcctgatcgc gcagcgccag gatggagtag
    34021 atagattcta ctgcacccgc cgcacctaac aggtgaccgg tcatagattt cgtggagctt
    34081 accaacacac ggcttgcagc ttcaccgaag atggttttca ccgcctgcgc ttcagcttta
    34141 tcgccagccg gcgtagaagt accgtgcgcg ttaacgtagc caatctgact cgcttcaatg
    34201 cctgcatcac gcagagcatt tgccattgcc agagctgcgc ctgcgccatt ttctggcggt
    34261 gacgtcatat gataagcatc gctgctcata ccaaagccga cgagttcagc gtaaattttc
    34321 gcaccgcgtt ttttcgcgtg ttcgtactct tcaagtacca gcataccggc accatcgccc
    34381 agtacgaagc catcacgctc tttatcccac gggcggctcg ccgcttgcgg gttatcattg
    34441 cgggtagata atgcacgtgc cgcgccaaaa ccaccaacgc ccagcggcgt actggctttc
    34501 tctgcgccac ctgcaaccat cacgtcagca tcgccatacg cgataatacg cgcagcatgg
    34561 ccaatgttgt gcacgccgga agtacaggcg gtcgcgatag agatgcttgg gccacgcagg
    34621 ccatacatga tagtcagatg acctgccacc atgttcacaa tcgttgacgg aacgaagaat
    34681 gggctgatct tacgtggacc accgttcatc agagatgtgt ggttttcttc gatcagtccg
    34741 aggccgccaa tcccggagcc aattgcggca ccaatgcggg ttgcgttctc ttccgttatt
    34801 tcaaggccag aatcctgcat ggcctgaacg ccagcgacaa ttccatattg aatgaaggca
    34861 tccatcttgc gctgttcttt gcgcgagata atgtcctcac agttaaaatc ctttactaag
    34921 ccagcaaatt tcgttgcata ggcgctagta tcgaaatggt cgattaggct gatgccactc
    34981 tgaccggcaa gcagagcttt ccaggtagac tctacggtat tgccgacagg agacaacatg
    35041 cccagtccgg tcacaactac acgacgctta gacacgtttg tcctccaagg agggaaaaat
    35101 gattctagtg ggacaaaaag ataaaactca ggcggtcgaa cgaccgcctg gagatgttca
    35161 cttacgcctg gtggccgttg atgtaatcaa tggcagcctg aacggtggtg attttctcag
    35221 cttcttcgtc cggaatctca gtatcaaact cttcttccag agccattacc agctcaacgg
    35281 tgtcaagaga atccgcgccc aggtcttcaa cgaaagaagc attgttggta acttcttcct
    35341 gcttaacgcc cagctgttcg ccgataattt tcttaacgcg ttcttcgata gtgctcatac
    35401 tcttaaattt cctatcaaaa ctcgctttcg cgatggtttt cgtagtgtat aaaatgttga
    35461 aaaatttgca actaaatccc ggcaggtctt accacgattt tacgttattt tgaggcctta
    35521 ccccccaata acgcaaatca ttttgcacta attattactc ataaccacgc agactgcgcg
    35581 caacatgagc attttgtgca aatcgcggtc agaccatgta catcccgccg ttcacatgca
    35641 aagtttcacc cgtgatgtaa gctgcttcgt cggatgccag gaatgcaacc gcgttggcga
    35701 tttcctgtgc gccgccgagg cgacccgcag gaacctgcgc caggataccc gcacgctggt
    35761 catcgctcag cgcacgtgtc atgtccgttt caataaagcc cggagcaaca acgtttacag
    35821 taataccgcg tgacgcaact tcgcgcgcca gtgatttact gaagccgatc aagcccgctt
    35881 tcgccgcagc gtagttggcc tgaccgccat ttcccatggt accaaccaca gaaccgatag
    35941 tgataatacg accatgacgc tttttcatca tagcgcgcat taccgctttt gacagacgga
    36001 aaacagatga aaggttagtt tcgataatat cgttccactc ttcatctttc attcgcatta
    36061 acaggttatc acgagtgata ccggcattat tgaccaggat atccacttca ccaaattctg
    36121 cgcgaatttt ttccagaaca gattcgatag atgccgggtc ggtcacattc aacatcagac
    36181 ctttgccgtt ggcacctaaa taatcactga tcgcctgagc gccattttca ctggtcgcag
    36241 tgccaataac tttcgcgcca cgggctgcga gcgtttcagc aattgcgcgg ccaattccgc
    36301 ggcttgcacc ggttaccagt gcgatttttc cttcaaaatt catgattttc ctcttttaaa
    36361 gctcgagcgc cgctgccatc gctgaaggtt cgttcagcgc cgaggcggtc agggtgtcga
    36421 caatgcgttt cgtcaggcca gtaagcacct tgcccgggcc gacttcatag agatgttcta
    36481 cgccttgcgc tgccatgtac tcaacagact tcgtccactg aaccgggtta tacaactgac
    36541 gtaccagtgc gtcacggatg gcatcaccat tggtttcgca tttcacatca acgttattca
    36601 caacagaaac tgttggtgcg ttaaaggtga ttttcgctaa ttctactgcc agtttgtcgg
    36661 ctgctggttt catcagcgca cagtgagacg gtacgctcac tggtaacggc agcgcgcgtt
    36721 ttgcgccagc agctttacag gcagcgccag cacgctcaac cgcttcttta tgaccggcaa
    36781 taaccacctg tcccggagag ttaaagttta ccggagaaac gacctgacct tctgcagctt
    36841 cttcacacgc tttcgcaata ggcgcatcat ccagaccgat gattgccgcc atagcgcccg
    36901 tgccttccgg tacggcttct tgcatgaact taccgcgcat ctcaaccaga cgcaccgcat
    36961 cagcgaaatc aatcacgcca gcgcaaacca gcgcggagta ttcccccagg ctgtgaccgg
    37021 ccatcattgc cggtgcttta ccgccctgct gctgccatac gcgatacagc gcaacagatg
    37081 cagtcaacag cgctggctgg gtttgccagg ttttattcag ttcttcagct ggcccctgct
    37141 gggtcagcgc ccacaggtcg tagcccagcg ccgcagaagc ttcagcaaac gtttcttcga
    37201 caattggata gctcgccgcc atatcagcca gcattccaac ggtttgagaa ccctgtccag
    37261 ggaacacaaa tgcaaattgc gtcatgtttt aatccttatc ctagaaacga accagcgcag
    37321 agccccaagt gaatccaccg ccaaaggctt caagcagaac caactgcccc ggcttaatgc
    37381 gcccgtcgcg tacagcttca tccagcgcgc acgggacaga ggccgcagag gtattaccgt
    37441 ggcgatccag cgtcaccacg acattgtcca tcgacatacc gagttttttc gccgttgcac
    37501 tgataatacg caggttagcc tgatgcggaa ccagccagtc cagttgagaa cggtcaagat
    37561 tattcgccgc cagcgtctca tcaacgatgt gcgccagttc cgttaccgca accttgaaga
    37621 cttcgttgcc cgccatcgtc agatgaattg aattctctgg attcacgcgg tcggcgtttg
    37681 gcagcgtcag caattcacca taactaccgt cggcatgcag atgggtggaa atgattcccg
    37741 gctcttcaga ggcagccagc accgcagcgc ccgcgccatc gccaaaaata ataatagtcc
    37801 cacgatctgt tggatcgcag gtgcgcgcca gtacatcgga accgacgacc agagcatact
    37861 tcaccgcccc agatttcacg tattgatcgg ctacgcttaa tgcataggtg aaacctgcac
    37921 aggctgctgc aacgtcaaat gccgggcaac ctttaatgtc caacatgctt tgaatctgac
    37981 aagctgcgct cgggaaagcg tgcgtagcag aagtcgttgc cacaacgatc aggccaatct
    38041 ggtctttctc aatgcccgcc atctcaattg cgcgtgtcgc cgcttcaaag cccatggttg
    38101 aaacggtttc gtttggcgcg gcaatgtggc gttcgcggat accggtacga gtgacaatcc
    38161 actcgtcaga ggtgtccacc attttttcca aatcggcgtt tgtccgcact tgttcgggca
    38221 gatagctgcc agtaccaata atcttcgtat acatgtacgc tcagtcactt ttcggttata
    38281 taccgtcact tgcaaactgc gagttcgctg gcagcgtcct gctaccgcag agttccgctt
    38341 ttgccaccgt ccagcagctc aaaaccagct gggtatacag attccaggcg agcggcaatt
    38401 cgctgaggaa cttgtcgctg caccgcctgc actgcctgtt caatcgcgac cgcaaaagct
    38461 cgctgattgg ctgcaccatg actttttatc accgtgccgc gcaatcctaa cagacaggcg
    38521 ccgttatact ggtcggggtt gaggtgactg aatcgcctcg tcaggctctt ttgtagccaa
    38581 cgctttaata acagtagcca ccacgaccgt tttttccctt caccctgaga tttcagcaga
    38641 gaaaggaaca tcctgacaac accttccatc gtctttaacg tgacatttcc tgtaaagccg
    38701 tcacaaacca gcacatctgt cttgccagtt aacaactcat tggcttcaag atagccgata
    38761 taattgatag aagggattgt tttaagcacc gctgaggcat cccgaatact gtcaagaccc
    38821 tttacttctt cttcaccaat attgagcaac gccacgcgag gattgggaat ttccaccacc
    38881 tcttcagcca gaactgagcc cataatggca aattgcacca acattgtgct gtcacaatcg
    38941 acgttggccc ctaaatcgag gaccaccgtt ttgccctttt gctgatgtgg taataccgtc
    39001 accagcgccg gacgctcaat cccctccagg ggcttgagta ataattttgc cagccccatc
    39061 agtgccccgg tattaccggc actgacacag gcttgcgctc gaccttcttt caccagctcc
    39121 agggccatac gcattgaact cccacgactg gcgcggatag cttgcgaagg ccgggcatca
    39181 ctggcgataa ctgactgcgc aggaataatc tgcagacgcg aacgttgttc aaagtcagct
    39241 ttagcaagta atggcgtgat ggcgtcggga ttgccgacta aaagaagagt gagttgcgaa
    39301 ttagaattca gtgcctgcaa tgctgcaggc actgtcacgg aagggccaaa atcccctccc
    39361 atgacatcta acgccagggt tagacgtgtc aaggtatcgt cgccgcccgg tttggtcttt
    39421 ccccagttgc ctggggaaaa tcctcactaa gcttcatcac gcagacgcgt gattacttag
    39481 cgatgacctt gcggccgcgg tagtaaccgt cggcagtgat gtggtgacgc aggtgttttt
    39541 caccagaagt tttgtctaca gacaggctgg tgactgcggt cagcgcgtca tgggaacgac
    39601 gcatgccacg tttggaacgg gttggtttat tctgttgtac ggccatggac cttactcctc
    39661 aattacggtt tatccccgat ccaacgggga gcaccaatta cttacgcttt aagctggcta
    39721 atacggcaaa tgggtttggc ttttgcactt cttcaggcag ttcaccaaag accatgtccg
    39781 cttcggacac ttcacagtgt tcagaatcat gcaccggaac taccggcaag gcgaggatga
    39841 tttcatcttc aaccattgca agcagatcga tttcaccgaa ttcgttaacc tcaatcggtt
    39901 catacgcttc cggcagtgct tcagcctgtt cgtctgaacg cacaggacta aaacaatacg
    39961 ttgtgtagac ctgatgagta aacggcttcc cgcaacgctg acactcgagc gttaccgtca
    40021 ccttcgcatc gccgtttaac actgcgagac gttggttatc gatagcgaac gacatggagc
    40081 attccacatc actgtccaca ctgactacgg attcggcgac gcgctcaacc tgatcagggg
    40141 tatagatacc ctggtaatca aggcgttttt gagccgtacg aaccggatcg agagtcaggg
    40201 gtaattttac cttttgcata gggcgcgcat attaactttg taacgtcata gagtcaaaga
    40261 aaaaggcagc ctgtggttgc cttttgccaa taattcgcac acattgcggg ttactgcttt
    40321 attttcgttc agtgctgata acgaacatca acctgcaaaa cgaagcgcat agtttaaaat
    40381 ggctatcatc aatacgctat atctgatgga aaaaaatatg cctaaactta ttttagcctc
    40441 cacctcgcca tggcgtcgcg ctcttctgga aaaactgcaa atctcttttg aatgtgcagc
    40501 acctgaggtc gacgaaaccc cacgcagcga cgaatcaccg cgacagttgg tgcttcgact
    40561 ggcacaagaa aaagcgcaat ctctggcgtc acgttatccg gatcatttaa ttattggttc
    40621 agaccaggta tgtgttcttg atggcgaaat caccggtaaa ccgttaacgg aagaaaatgc
    40681 ccgtctgcaa ttacgcaaag ccagcggcaa tatcgtcacc ttctatactg gactggcgtt
    40741 gtttaattcg gcgaatgggc atctacaaac agaagtggag ccttttgacg tccatttccg
    40801 tcacctaagc gaggcggaga ttgataatta cgtgcgtaag gagcatcccc tgcactgcgc
    40861 gggtagcttt aagagtgaag gatttggcat tacgctgttt gagcgtttag agggacgtga
    40921 ccctaacacg ctggttggtt taccgctaat cgcgctgtgt cagatgttac gtcgggaagg
    40981 gaaaaacccg ctgatgggat agttgtttgc gccggatgcg gttacgtcgc atccggcaat
    41041 cggtgcagac ggcctgatgc ggcgtaaacg ccttatcagg ccaaccgccc attatcaggc
    41101 ttatatgctt gtattagcgc gcgttacgca gcttttgcaa acaacgcttc aaaccttcat
    41161 ccatcggcgc ttcgatacgc atcacctcac cggtccccgg atgggtaaac ttcaacgctg
    41221 cggcgtgcag gaacagacga tttaatcccg tgcctgcttc agtgagctgt ctgtcaaatt
    41281 cacggtcacc gtagcgatcg tcaaaggcaa tcggatgacc cgcatactgt gtatgcacac
    41341 ggatctgatg agtacgtccg gttaccgggc tacaacgcac cagggtggca aatgcatagc
    41401 gttcttccac tttaaagcgt gtttccgacg gtttgccttc ctgactgaca cgcacgatac
    41461 gttcgccgct ttgcagaata tttttcaata acggcgcttg aacgctcttc acatgcgact
    41521 gccactgacc gcgcaccagc gccaggtaat ctttttgcat tcctttttca cgtaattgct
    41581 catgcagaga acgcaacgcc gagcgttttt tcgctaccag caacacacct gaagtgtccc
    41641 ggtcgagacg atgaaccagt tcaaggaacc gcgcttccgg gcgcaacgcc cgcaaacctt
    41701 cgataacgcc gaagcttaaa ccactgccgc catgtaccgc cgtaccggaa ggtttattca
    41761 gcaccaggat atgatcatct tcatataaga tgacgtccgc cagcgccgcc accttttgca
    41821 gatgtggcga aaccgcctct tcttcccgct cagcaacgcg aaccggtggg atacgcacct
    41881 catcacccgc ttcgagttta tattcaggct taatacgttt tttgttcacc cgcacttcac
    41941 ctttacgcaa aatacggtaa atcatacttt ttggtacgcc tttcaattgg gtacgcaaaa
    42001 agttatcgat acgttgcccc gcttcgtcag cggtgatagc aacaattttt acggatggag
    42061 tctctgtttt catggtgggc gattctaatt agccaacagg attcgcgcca ctcatttttc
    42121 tatgcttata tttagttttg caccttatta cttcactacg tgatcacttt attgatggtt
    42181 attaaaccaa tcaccagcaa gaagtgaaaa aactgtgagt aagcgggtga taaatggtaa
    42241 aagtcatctt gctataacaa ggcttgcagt ggaataatga ggccgtttcc gtgtccatcc
    42301 ttgttaaaac aagaaatttt acggaataac ccattttgcc cgaccgatca tccacgcagc
    42361 aatggcgtaa gacgtattga tctttcaggc agttagcggg ctgcgggttg cagtccttac
    42421 cggtagatgg aaatatttct ggagagtaat acccagtctg tttctttgat aattgcgctg
    42481 tttttccgca tgaaaaacgg gcaaccgaca ctctgcgcct ctttgagctg acgataaccg
    42541 tgaggttggc gacgcgacta gacacgaggc catcggttca cacccggaaa ggcgttactt
    42601 tgcccgcagc ttagtcgtca atgtaagaat aatgagtaag ttacgatgaa aagaatgtta
    42661 atcaacgcaa ctcagcagga agagttgcgc gttgcccttg tagatgggca gcgtctgtat
    42721 gacctggata tcgaaagtcc agggcacgag cagaaaaagg caaacatcta caaaggtaaa
    42781 atcacccgca ttgaaccgag tctggaagct gcttttgttg attacggcgc tgaacgtcac
    42841 ggtttcctcc cactaaaaga aattgcccgc gaatatttcc ctgctaacta cagtgctcat
    42901 ggtcgtccca acattaaaga tgtgttgcgt gaaggtcagg aagtcattgt tcagatcgat
    42961 aaagaagagc gcggcaacaa aggcgcggca ttaaccacct ttatcagtct ggcgggtagc
    43021 tatctggttc tgatgccaaa taacccgcgc gcaggtggca tttctcgccg tatcgaaggc
    43081 gatgaccgta ccgaattaaa agaagcactg gcaagccttg aactgccgga aggcatgggg
    43141 cttatcgtgc gcaccgctgg cgtaggcaaa tctgctgagg cgctgcaatg ggatttaagc
    43201 ttccgcctga aacactggga agccatcaaa aaagccgctg aaagccgccc ggctccgttc
    43261 ctgattcatc aggagagcaa cgtaatcgtt cgcgccttcc gcgattattt acgtcaggat
    43321 atcggcgaaa tccttatcga taacccgaaa gtcctcgaac tggcacgtca gcatatcgct
    43381 gcattaggtc gcccggattt cagcagcaaa atcaaactgt acaccggcga gatcccgctg
    43441 ttcagccact accagatcga gtcgcagatc gagtccgcct tccagcgtga agtccgtctg
    43501 ccatccggtg gttccattgt tatcgacagc accgaagcgt taacagccat cgacatcaac
    43561 tccgcacgcg cgacccgcgg cggcgatatc gaagaaactg cgtttaacac caacctcgaa
    43621 gccgccgatg agattgctcg tcagctgcgc ctgcgtgacc tcggcggcct gattgttatc
    43681 gacttcatcg acatgacgcc agtacgccac cagcgtgcgg tagaaaaccg tctgcgtgaa
    43741 gcggtgcgtc aggaccgtgc gcgtattcaa atcagccata tttctcgctt tggcctgctg
    43801 gaaatgtccc gccagcgcct gagcccatca ctgggtgaat ccagccatca cgtctgcccg
    43861 cgctgctccg gtaccggtac cgtgcgtgac aacgaatcgc tgtcgctctc tattctgcgt
    43921 ttgatcgaag aagaagcgct gaaagagaac acccaggaag ttcacgccat tgttcctgtg
    43981 ccaatcgctt cttatctgct gaatgaaaaa cgttctgcgg tgaatgcgat tgaaacgcgt
    44041 caggacggtg ttcgctgcgt gattgtgcca aacgatcaga tggaaacccc gcactaccac
    44101 gtgttgcgcg tgcgcaaagg ggaagaaacg ccgaccttaa gctacatgct gccgaagctg
    44161 catgaagaag cgatggcgct gccgtctgaa gaagagttcg ctgaacgtaa gcgtcctgaa
    44221 caacctgcac tggcaacctt tgccatgccg gatgtaccgc cagcgccaac cccagctgaa
    44281 cctgccgcga ctgtcgtagc cccagcaccg aaagctgcaa cggcaacacc agcagctcct
    44341 gcacaacctg ggctgttaag ccgcttcttc ggcgcactga aagcgctgtt cagcggtggt
    44401 gaagaagcca aaccgaccga gcaaccaaca ccgaaagcag aagcgaaacc ggaacgtcaa
    44461 caggatcgtc gcaaacctcg tcagagcaac cgccgtgacc gtaatgagcg ccgcgacacc
    44521 cgtagtgaac gtactgaagg cagcgataat cgcgaagaaa accgtcgtaa tcgtcgccag
    44581 gcacagcagc aaactgccga gacgcgtgag agccgtcagc aggctgaggt aacggaaaaa
    44641 gcacgtacca ccgacgagca gcaagcgccg cgtcgtgaac gtagccgccg ccgtaacgat
    44701 gataaacgtc aggcgcaaca agaagcgaag gcgctgaatg ttgaagagca atctgttcag
    44761 gaaaccgaac aggaagaacg tgtacgtccg gttcagccgc gtcgtaaaca gcgtcagctc
    44821 aatcagaaag tgcgttacga gcaaagcgta gccgaagaag cggtagtcgc accggtggtt
    44881 gaagaaactg ccgctgccga accaattgtt caggaagctc cagctccacg cacagaactg
    44941 gtgaaagtcc cgctgccagt cgtagcgcaa actgcaccag aacagcaaga agagaacaat
    45001 gctgataacc gtgacaacgg tggcatgccg cgtcgttctc gccgctcgcc tcgtcacctg
    45061 cgcgtgagtg gtcagcgtcg tcgtcgctat cgtgacgagc gttatccaac ccagtcgcca
    45121 atgccgttga ccgtagcgtg cacgtctccg gaattggcct ctggcaaagt ctggatccgc
    45181 tatccaattg tacgtccgca agatgtacaa gttgaagagc agcgcgaaca ggaagaagta
    45241 caagtgcagc cgatggtgac tgaggtccct gtcgccgccg ctgtcgaacc tgttgttagc
    45301 gcgccagttg ttgaagaaat ggctgaagtc gtagaagccc ccgtaccggt tgccgaaccg
    45361 caaccggaag tggttgaaac gacgcatcct gaagtgattg ccgccgcggt aactgaacag
    45421 ccgcaggtga ttaccgagtc tgatgttgcc gtagcccagg aagtcgcaga acacgcagaa
    45481 ccggtggttg aaccgcagga agagacggca gatattgaag aagttgccga aactgctgag
    45541 gttgtggttg ctgaacctga agttgttgct caacctgccg cgccagtcgt cgctgaagtc
    45601 gcagcagaag ttgaaacggt ggccgcggtt gaaccagaga tcaccgttga gcataaccac
    45661 gctaccgcgc caatgacgcg cgctccggca ccggaatatg ttccggaggc accgcgtcac
    45721 agtgactggc agcgccctac ttttgccttc gaaggtaaag gtgccgcagg tggtcatacg
    45781 gcaacgcatc atgcctctgc cgctcctgcg cgtccgcaac ctgttgagta ataattagct
    45841 caaagtaatc aagccctggt aactgccagg gcttttttat ttcatgtttg aatcaacata
    45901 ttaaaattca tcaaatggca tccttgctaa ccaacaatgc aaaataggca aaaaaatgcc
    45961 cgccccggcg atcccagagc gggcagacaa aacataccca gtttcatgat atgttttaaa
    46021 gcgagcttat ttgttgagct ggaagagcga caatccctgc atatcggtaa atgctttata
    46081 cgatgcctgc aatgccgttt gctgcatgat gtaagatgaa atagttgcat tccagtccac
    46141 atcaaccaga tcgctcatct gctgcgtttg ccctaaagcg cgatcgctac ctaaggaatc
    46201 cagcgactcc agtttgttca gctgcgtgcc taattccgcg cgaacggtaa ggacattatt
    46261 cagcgagttt ttcagtccac ggttggtttt atccaatgcc gcagcggcga tttctttatc
    46321 cgcttcgcta tccgcgaccg gcgttttcag ggctgcgatg gcgctatcca gcatggcaaa
    46381 aagattggtt tcagaagcgc taccgtctgg ttccgctacc gcgttactgg taatactgtc
    46441 gaaaattttg tcgcccgtat gtcctatcac catcgaacgc gaagcatcga cctgttgctt
    46501 aatactttct gttccgccaa cataatcacc gttagcctca ctaaacggcg cagtctctgt
    46561 tttataaccg gcaaaaatgt agcgcccatt accgtcagtg gtgttcgcca gattcagcaa
    46621 ctggtcacga agcccctgaa tatccgtagc cagcgaggcc cggtcatcgt cactcaaggt
    46681 gccattgctg gcgtagacaa ttttttcctg agcattctgg atagcagtgg tgacctggct
    46741 aagtacactc tcttccagtg acactttttg agtggcgaaa gtacgtgcca gcgtgtactg
    46801 gctgttttgc gcctgtgcct gggagagaac aacagcttgt gatgcagcaa tgggatcgtc
    46861 tgaagggtta acgactcgct tacccgtcga catctgctcg ccgtacttca tccattctgc
    46921 ctgagaattg gtgatgccgc gcatgttttg ctggtacatc atctgggtac tgaaacgcat
    46981 tttatctccc cttagcgaat gttaatcagc gcatcaaaaa tcgcgtttgc cgtctgcaga
    47041 acctgcgcat tcgccaggta atactgctga aaacgttgca gatttccgta ctcctcatcg
    47101 agattgacac cggaaatcga ctgctgctga ttggaaagct gcgtcaccac attaccttgc
    47161 gtggtgctac tggttttcaa cgtcgcggtt ttattaccga tatcactcac taacgaggca
    47221 taagcgtcgt taaaggattt cgcaccgccc accgttttac tgttgctttg cagatccagc
    47281 agggcctgac cgttgcggtt gtcgctatca cccgcatctt cttcgctcgc catcgcgatt
    47341 ttcgcttcgt cggtgattaa tacatccatg ttgacgatgg cgtcacttac tggtttcagc
    47401 gtgaagctgt cgttaacggc aggcgttcct gtaaacgtca actccagacc atcaaatgcc
    47461 actttaccgt tggcatctgg tgtcgccgta aaagtggtat tgctggcaag gcgggtgacc
    47521 tgccactgat tattatcgaa cgagattttg taatctgtcg ccagtaccac ggaggcatca
    47581 gttaccgtgg caccgatcgc aacgtcacct ttgtttttcg tgttttgcag aaccgcgggc
    47641 ttaccgatag taaagaaatc ttcaccagca tcgccgttag catcaaatcc ggctttgtgt
    47701 tggctgttga aagcctcggc aaatgccagc gccagttgtc caagcgtatt acgcgtctgg
    47761 tccagatcct gagaacggaa tgtcagaatg ccgcccagcg acccggtatt cagtaatttc
    47821 tccgggatct caatattgcc tgccgtccca tcaacgtaag cgacagtcgt acgagaaggg
    47881 tcggcgctgg aaggaactgc cgccagttgc cgcgccgtac ttccctgaac cagtgagtaa
    47941 ccattggcat cgtgatgtta taagtaccgc catcctgaac gctgacttct acgccaacaa
    48001 tctggtttaa ttcgctcacc agttgatcgc gttgatccag cagattgtta ggtgacgccc
    48061 ctgcccccac gcctgtcagg cgcgagattt gatcgttcag gctggcaatt tgtttagcgt
    48121 aattgttgat ctgatcaacg ctggcaccta tcgcgatatt gacctgtttg tcctggtcgc
    48181 gcagatattg atcggtggtt ttaaactgat tcaccaaccc ttctgatttc ccaatcagcg
    48241 cctggcgcgc cgccgggtct tccgcgttac tcaccagcgt ttgcaggctg gtgaagaaat
    48301 cctgcatctg tgttgccagc gaagaggtac tggtggagag catattgtcg attttcgaca
    48361 tctgctcata gcgggcggtc aggccgctac tttgcgtctg cgcagcacgt aactggttgg
    48421 taataaacgt atcatactca cgctgcacac cagaaacgta gacgccattg ccaacccagc
    48481 cgccagcgcc caacgtgcta ttggcctgcg ccataatagt ggtttggcgg gtatatccgg
    48541 caacgttata gctggagata ttattacttg ccgtatttaa cgccgcctgg gccgcgttca
    48601 gtccgctcat ggcgttattg atcaagctgg acatggaggt tccttataag ccttcaatta
    48661 cagagtatta tcggcagcga cccgccggac ttgagttatt cagaacagat tatcaatgtt
    48721 catgctgtag gttttgctca ccttgtcgct tatcgatttc atctgctgga tcatgttggt
    48781 gagtttacgg gcatagtgag gatcggtagc atagcccgcg tcctgtagga cctgcgcccc
    48841 ctgttccgca ctcgcggcgg tcgtcacggc ggcgtagcgc gggttacgtg ttaacagccc
    48901 aacgtaatcc gacaatgctt ccagatacga gctgtagacc cgaaacttcg cttttacttt
    48961 cttcgcttcg ccgttttcat attcagtcgt ggtgatttca gtgacttgcc ctttccagtt
    49021 gccagaggct ttgacaccaa acaggttata gcttggctcg ccgttttcgc ggcggatttg
    49081 ccgttgcccc caaccagatt caagcgcagc ctgagcgagg atcaaatgat gtggcacacc
    49141 gctttgctgg cttgccagtt gggcgggcag cgagagttgc gcgaggaatg ctctactgtc
    49201 acccggcagc gaatcatcgt agttacgtgg tacggccttt tgcaccagct gcgaaagcgt
    49261 ctggttttga taacgcacca cggtttcgag cgggaatttc atcggtgctg ctggcgtgga
    49321 ctcctctggc aatggttgtt ctggcgtcat ctgtttaacc atcatctctg caagccccag
    49381 acctttgccc gccgtcatct gttgggcaat ttgctggtca tacatactgg tatacagacg
    49441 agtgtgctcg ctgctgaaca ggccatcttt tggtaaagcg tcgcgcatgc ttttcaacat
    49501 catctgcacg aacatccctt ccacctgacg ggccaccgga cggatatttg ccgccggatc
    49561 ttcgctcgct ttcgccttta gttcgttgag tgattgcgca tcccaggccg cacttgccag
    49621 tagtttgctg tcgctgatca tcagatgatt tccagttttg cccgcagaca tcccgcactt
    49681 tgcattgatt gcaaaataga catcagatcc atcggcgtag cgcccagcgc attgagcgca
    49741 cgcaccacgt tattgaggct ggcgctggaa cgtacgcttt gcagcgaacc gccgctctgg
    49801 cgtaaatcga tctgcgtttg tggagtaaca acagtctgtc caccaccaaa cggtgtatct
    49861 ggctggctga cattggcctg acgattaact gttactgaga gatttccctg cgctaccgcg
    49921 cagctgtcga gggtcacttc gcgattcatc accaccgaac cggtgcgcga gttaatcact
    49981 actttagcgt cctgcggggt gacattaacc tgcatattct ggatatcggc aaggaaacgg
    50041 acctgggaac tgttgccact tggtacgcgc acctgaatag tccgcgcatc taacgcggtg
    50101 gcgctgccat atccacgcac gcggttgatg gtgtcagcga tttgctgcgc catgctgaaa
    50161 tcttcgtcgt taagttgcaa attaagggta ttcccgacgc caaactggct gggcaattca
    50221 cgttcaataa ccgcaccatt ggtgatccgt ccaccgttca gttggttaac ctgaacactg
    50281 ctaccgcctg cggaggctcc tgcgccgcca accagaatat tgccctgcgc cagcgcatac
    50341 acctgactgt caacgccctt aagcggtgtc atcaacaacg tacctccacg caagcttttg
    50401 gcattaccca tggaagaaac caccacatcg atggtttgcc cctgacgtcc aaacggagga
    50461 agtgacgctg tcaccattac cgcagcgacg ttttttagct gcatattggt gcccgtcgga
    50521 acggtaattc ccagctgtga gagcatgtta ttaagcgttt gtgtggtaaa cggcgtctgg
    50581 gttgtctggt caccagtgcc atccagcccc actaccaagc catagccaat cagtgagttt
    50641 tgccttaccc cctgaacact ggtgagatcg cgaatacgct cagcctgagc cgccgtcgta
    50701 accagtagaa gaattaatgc agagagaaat ttaatcacca cagcctcact tacattggcg
    50761 acaggttaag gaagaaacgc tgcaaccagc ccatattttg cgcttcgtta atgtagccat
    50821 tgcctacgta ttcaatacgc gcatccgcca cctgagtaga cggtacggta ttgctgccgc
    50881 tgatagtgcg tggattaacc acgcccgaga agcgaataaa ttcggtaccc tgattaatgg
    50941 caatctgttt ttcacccacc acatgcaggt tgccgttgac cagtacctgg tcaaccgtca
    51001 ccgtcaacgt gccgctaaag gtattgctgg cattggcccc gccctttccg ttgaacgtgt
    51061 taccaccgga ggcttcgaca tcggcacgag cgttaccaaa cagcccctgc aaatagcgcg
    51121 gcacagtatc aaagccaaaa ttagttttac cgtcacggct ggcattcgca gaggagcttt
    51181 tgctggcgct gacgttctcc tgcaacacga tggtcagcgt atcgccaata ttgcgtggtc
    51241 gacgatcttc aaacagcggt tgatagccat agttaatcgg ctgagcagac tggaaaatag
    51301 aaccgttggc gacgggcgtc ggaccgggaa ccggttgtgc actggtcgcc ccctgcacca
    51361 gcggcgtgga gggtatccag gcgcagccgg ttagtgaaag caccaacaag ctggaaatgg
    51421 cataagtatg cgcagcgttt ttttgcatgg ctatcatctt caaaaatcag taaaccggtg
    51481 aacctgccac cggttatgcc ttagagttgc gtcagttttt gcagcatctg atcggtggtg
    51541 gacaccgctt tactgttgat ttcgtaagcg cgttgcacct gaatcatatt gaccagttct
    51601 tccgccacgt tgacgttaga cgtttcaaca tacccttgat acagcagtcc cgcgccgttc
    51661 aggcccggcg tgctttcgtt cggtgcaccg gaggattgcg tttcggtgta gaggttttcg
    51721 ccaatgctct ccagtccggt atcattcata aaggtggtga gattgagctg cccaacctga
    51781 accggagctg cctggccttg ttgggttacg ctgaccacgc catcacgacc gatggtgata
    51841 cttaacgcat tcgccggaat ggtgatcgct ggctgcacct gaaaaccacc agccgtcacc
    51901 agctgcccgt tctgatccac ctggaaagag ccgtcacgga tataggctga tgagccatct
    51961 ggcaacatta cctggaaaaa gccctgccct ttaatcgcga catctttgct gttattggtc
    52021 tgcgacagat tgccctggct gtgtaagcgt tcagtggcga ccgggcgtac ccccgtgccg
    52081 atttgtaatc cggagggtaa ggtggtttgt tcggaagact gtgcccccgg ctggcgaatg
    52141 gtttgataaa gcagatcttc aaacaccgcg cgctgacgct taaaaccgtt agtactgacg
    52201 tttgccaggt tgttggcaat gacgtccata ttggtttgct gggcgtcaag gcccgttttg
    52261 gcgatccata atgaactgat catataatgt cctgtagtgg attgctgaca agcagagcag
    52321 aagcacgcga cagtgtgaat ttcccctcac cctaaccctc tccccaaagg ggcgagggaa
    52381 tagtccgcgc gcttttattg caatgtcatc aatcgttcaa ttaactcatc gacagcagtt
    52441 ggttggcacg gcctgcgtta tcatcgacgc tgctgatcac cttcatctgc atttcaaaac
    52501 gccgcgcgct ggcaatcatg tcgctcattg ccgcaacggc attgacgtta ctgccttcca
    52561 gaacccccga catcacacgc aaggttggat ctgcctgcag taccggccct cgcgtggcct
    52621 gggtttctgc gcttaaacga aaaatgccgt cgtcaccgcg ctgcacttcg ctgcccgtgg
    52681 ctttcaccag tttcagacgc cctactggcg caactgtatt tgccggatcg cccggattga
    52741 gcgccgagat tgtgccatcg gcagcaatcg tgatttccgc cccttccggc acagcaattg
    52801 gccccgcctc gcctatcacc ggatgcccct gaattgtcag ttgcccggtg ggatcaacct
    52861 gaatgctgcc attacgcgta tacccttcgc tgccgtcagc ggtctgcacg gccagccatc
    52921 ctgctgcaac gcaacgtcca gcgggcgcga ggtgtaatcc atcttgccgg gcgtcatgtc
    52981 tgcacccggt gttgacgccg ttaccagcgt gcgcgtgggc agtgaaagcc cttccaccgg
    53041 cactgcgcgc aaggcgttta actgcgcgcg aaaaccgggc gtagaggcat tggccagatt
    53101 actggcggtt accgcctgtt gattcagcgt ctggctggct gctcccatcg cagtataaat
    53161 tgcgtgatcc attgagctat cccgtcagcg attagcgtaa gttaacccgc gtgttgagga
    53221 tctggtcctg ggttttgatg gtctgggcgt tagacttata gttacgctgg gcaacgatca
    53281 tattgaccag ttctttactg agatcgacgt tggacgcttc cagcgcaccg ttggtcaggg
    53341 tgccaaagtt tcccgtcccg gctgtcccca acagcgccac gccagaagat tgcgtcgcag
    53401 accagacgtt gtcgccttcg gatgccagac cttcgttgtt ggcaaagttc gccagtacaa
    53461 tctgccccag cagttgggtt tgttcgttgg aattgttgcc gacaaccgta ccgtcatcat
    53521 tgatttgata actcaccaga tcgcccggtt tgtagccatt ctgggtggtt gccacaatgt
    53581 tgttagcgcc ggtattttgc tgcatggagt tgaggaagct cagactaaac gtggcgggtt
    53641 ctgcaccgtt aattgcgccg gttgcgatat tattcgccat cgcaccatcc actaatgtgc
    53701 cattagcatt aaattccagt gttgtcgctg tcttcgcaat gctgtttgga tcactgctat
    53761 cctgggtgta gacctgccag ttattatccc cggtcttcac aaaatagacg ctcatgtcat
    53821 gagcattacc ctgactgtcg aaaacagtca ccgaaccttt tttgttatag ctatccgcat
    53881 tgctggcgct gaatggcgta acagtaggaa gcggatcact ggaattcagg ttgatctgca
    53941 tcgacgccgt ggtggtagtt ttcgctgcca tcagggtatt cgggatcgaa atattggtcg
    54001 gattcgcccc ttgctgaata gtcggcggcg taccggttac cgggtaaccc gtcagctgta
    54061 aaccttgcgt attcaacagg ttacggtttt catccagctt aaattgtccg ttacggctgt
    54121 agaacaccga accgttgctg tctaccagac ggaaaaaacc gttctggctg atagcaacgt
    54181 ccagacctcg cccggtgttg gtggtcgtgc catcggtaaa gtcctgagtg ataccggcaa
    54241 cttttacccc cagtcccact ttcgaaccgg caaacatatc ggcaaaagag gccgtgcctg
    54301 atttaaagcc gtaggtggcg gagttggcga tattgttgcc aataacatcg aggttggtgg
    54361 cggcagcgtt taatccgcta accgcttgag aaaaggccat gactgactcc tgaaatgtga
    54421 aggcttaaat tatctgccgt acttcgtcga gggtggtggt gccgtaagtg ccgagatcca
    54481 gcgtattacc gctgttgccg cggatcacac cctgcaccag agcaaactgc agcggctggg
    54541 caaccagttg tgtaccaccg ttactggcgc taatcgctac attgtaagaa ccgttcggcg
    54601 cagtgctgcc atcagtcaac gaaccgtccc aggtgaaact gtgaactccg gcggtcagtt
    54661 caccaatatc aatggtgcgc acaaccgcgc catttttatc ggtgatggtg gccgtaactt
    54721 tgtctgccgc ctgttgcagc tcaacaccaa acggcgtggt cgtggtcaca gccccttctt
    54781 cactgccggt tcccgcaaga acagttgtgc cggggatcat cacgccgtga ccgatcaggt
    54841 tactggcctg taacgactgg ctgttatcaa tctgtccgga aatagatccg agcgtggtat
    54901 tgagtttttc aatcccgctg accgtgctga tttgtgccaa ttgcgacgtc agctcgttgt
    54961 tttccattgg attggtcggg tcctggtttt tcagctgcgc caccagcaaa gtcagaaaac
    55021 tgctttgtaa atctgcggcg ttgctgcccg tgagcgaact actgctggtg gtactgacgc
    55081 cggtatttgt cggatcggtg gtggttaccg caatggacat agctttctcc tttattgacc
    55141 gagcgtaagg gttttcagca tcatgctttt caccgtgttg agcacttcaa cattggcctg
    55201 atagctgcgt gacgccgaca tggtgttaac catctccccg acgacatcaa cgttcggcat
    55261 ttttacgtag ccctttgcat ctgccagcgg attacctggt tcataaacca gtttgtccgg
    55321 ggcctgactt tcttaacatc ggcaaccttt acgccgcctg tcgcagcacc tggtgcagcg
    55381 ttaacctgga ataccacctg ttttgcccga tatggctgtc catcaggacc ggtcacgcta
    55441 tcagcatttg ccagattact ggccgccacg ttcaggcgct gggactgggc agttaacgcc
    55501 gacccggcga tatcaaaaat attcagcagt gccatccgtt aatttccgct ctgtaaaacg
    55561 ttcatcatgc ctttgatttg cccgctcaac gcgctaaggc tcatctggta ttgcaggctg
    55621 ttatcggcaa actgggtgcg ttcgcgatcc atatcgacgg tattaccgtc aagcgaaggc
    55681 tggtccggaa tacggtattg cagttctgcg gtaggaggcg tcagcgcctg cgccgggatg
    55741 tgttgcgttg aggtcatcgt cagtgcaacc acactggttg catcccgtcc acgttgcatg
    55801 acttttttaa gttcactggc aaaatcgata tcgcgcgcct gataaccagg ggtatcggca
    55861 ttggcgatgt ttgctgccag cacttcctga cgctgggcgc gcagattgag cgcctcttgt
    55921 tgaaaacgta aggcggcgtc gagcttatcg agcatatctc ctccgcaggt atcaaaattc
    55981 tgccatcagc ttaaatgcct ttacgcgcgc gttatcggcg gaataaacgc aaaatgggtc
    56041 gctatttatg ccgttgatgg tcattgcgga caggtacaat tcacgttgta gaaatggctg
    56101 ggggcgaaaa tgctgacaat aaaacgtagc gtggcgatca tcgcgatact gttcagtccg
    56161 ttaagtacgg cgagcaatct cacatcgcaa ttgcacaact tttttagcgc ccaactcgcg
    56221 ggggtaagtg atgaggttcg tgtttctatt cgtacagcgc ccaatctact accgccatgc
    56281 gagcagccat tgctttcgat gagcaataat tcccgcctgt ggggcaatgt gaatgtgttg
    56341 gcacgctgcg gtaacgacaa acgatattta caggttaatg tgcaggccac aggaaattat
    56401 gtggttgccg cgatgcctat tgtgcgggga ggaaagctgg aagctggcaa tgtcaaactg
    56461 aaacgtggac gcctggatac cctgccaccg cgtacggtgc tggatatcaa tcaacttgtt
    56521 gatgccgtta gcctgcgcga tctatcaccc gatcaaccta tccagttaac ccagtttcgc
    56581 caggcatggc gggtaaaagc gggacaacgc gtcaatgtga tcgccagcgg tgatgggttt
    56641 agcgccaacg cagaaggtca ggcgctgaac aatgcagccg tcgcacagaa tgcgcgggtg
    56701 cgcatggtat cgggacaggt agtcagcggc gttgttgatg cagatgggaa tattcttata
    56761 aacctgtaag ctgttaaaga ttacccgtcc cttgccgata aataagcaac acatgataaa
    56821 agcgccctca atgaggaata aaccatgagt attgatcgca cttcgcctct gaagcctgta
    56881 agcaccgttc aaccgcgcga aaccaccgac gcgccggtaa cgaacagccg ggcggcaaaa
    56941 acaaccgcct ccaccagcac cagtgtgacg ttaagcgacg cgcaagcaaa actgatgcaa
    57001 cccggcagca gtgatatcaa tcttgaacgt gtcgaagcgt taaaactggc gattcgtaac
    57061 ggtgaactaa aaatggacac cggcaaaatt gccgatgcgc tgatcaacga agcgcagcaa
    57121 gacttgcaga gtaactgatc gtatgacacg tcttgcagag atcctcgacc agatgtccgc
    57181 tgtgcttaac gatctcaaaa cggtaatgga tcaagagcag caacatctct ctatggggca
    57241 gatcaacggc agccagttgc aatggattac agaacaaaaa agctcactgc tggcgacgct
    57301 ggattacctc gaacagttac gcaagaaaga acccaataca gcaaatagcg ttgatattag
    57361 tcaacgctgg caggaaatta ctgtgaaaac gcagcaacta cgccaaatga atcaacataa
    57421 cggctggtta ctggaaggac agattgagcg caatcaacag gcgctggaaa tgttgaaacc
    57481 gcatcaggaa ccgacgctat atggggcgaa cggtcagacc tcaacacccc atcgcggcag
    57541 taaaaagatt tcgatctgaa tattatctcc ggcctgcact gcaggccgga atgcattgtt
    57601 acaccgtccg gcgggcaaat tctttaactt tgaagcccag taccgccagt gcagcgaagt
    57661 acgcggcaat ccccgccagc acgaccgcca ttaaacgcag taagcgccag ggcatggtac
    57721 ccaatgacca ctccggcatg atatgtaaca tacctaaaag cacgccagac atcaccagta
    57781 ccgccaccac cagacgcaac agaaacgcca tccagccggg ttgcggagta aagattttct
    57841 gcttacgcaa ctgccagtaa agcagcgaag cattcagaca cgccgccaga ccaatagaaa
    57901 gtgacagccc ggcatgtttc aacggaccaa taaacgccag gttcatcaat tgcgttaaaa
    57961 tcagcgtaac gatggcaatt ttcactggcg ttttaatgtc ctggcgggaa taaaagccag
    58021 gagccaacac tttcactaca atcaggccaa tcaaacccac cgagtaggca attaacgccc
    58081 gctgggtcat cagcgcatca aacgcggtaa atttaccgta ctggaacagc gaaacggtta
    58141 acggacctga aagaatgccc aacgcaaccg cactcggcag tgccaacagg aaacaaagac
    58201 gcaaccccca gtccatcaaa cggttgtatt catcatgatt gccactggca aaacttttcg
    58261 acagcgacgg cagcaaaatg gtgccaagcg ccacgcccag cacaccggag ggaaactcca
    58321 ttaagcggtc ggcgtaatac atccaagaca ccgaaccgga agcaagaaac gaggcaaaaa
    58381 tggtgttgat gattaaggag atctggctca cagagacgcc aaggatcgcc ggtcccatct
    58441 gtttcaccac gcgcattgct ccagcatcgt ggaagttaat gcgcggcagg accagcatgc
    58501 cgatcttctt caggtgcggt agctgataca ccagctgcag gatgccgccg accgtaacag
    58561 cccacgccag cgccagcacc ggtgggttaa agtacggtgc ggcaaacagc gcgaaaccaa
    58621 tcatgctgat gttaagcagt gttggcgcaa acgccggaat cgagaagcgg ttccacgtat
    58681 tcagaatcgc tcccaccagc gacgccaggg agatcagcaa gatataggga aaggtaatct
    58741 ttagtagctg gctggtcagg gcaaatttgt cagctgtgtc agcgaagcct ggcgcggtca
    58801 ccatgatcac ccacggtgcg gcgagcatgc cagcgaccgt cacaaccgcc agcgcaagtg
    58861 tcagcaggcc agaaacataa gagacaaaga cccgcgtggc gtcttcaccc tgcttacttt
    58921 tatattccgc cagaatcggt acaaatgcct gggaaaatgc cccttcggca aagatacggc
    58981 gtaacaagtt aggaagttta aaagcgacga aaaaggcgtc ggttgccatc cctgcgccaa
    59041 agattctggc gacaattgcg tctcgtgcga agccaagcac acgcgaaaac atggtcatcg
    59101 agctgacggc ggccagcgat tttaataaat tcatcggtgt tctaatccgg acccacgggc
    59161 aaaacgcctg caaggcaggc gctgaaagag gcgaatagtc tacctagatt atggtgaatt
    59221 gctaccgcca gatgttacag ggtttattca ctcatcgcat cgcgccagat cttgtcaacg
    59281 atacgttgcg ccagcactgc ctattcgccg gcggtttgcg gaactgtctg attttgcacg
    59341 cattcaatga agtgccgcgc acagccgaca aatccacgtt gctcaagagt gctctgccaa
    59401 ccaggaatcg gtttattcac tacaccctgc ccgcgctcct cacgccattc gcgcatatcc
    59461 gtaatgtcga tgagcgcacc gtcagtcacg gcctgcacgg tttcacgctg acttccggca
    59521 cggcgatgca tacaagtggt gatctgcaaa ggaccggccg aaaaatggtg ctcggcaaac
    59581 agcatttcgc cagcgtcgtt agtcagtagc gtaccgccat ccagagaggc tttgccgccc
    59641 gacaaccaca gcgcggtatc caccacatgc agataatcat ccagcaacgt gaaataaaga
    59701 tcgtgtggcc cgacgctatt gctacgatgt ttatccattc ttagcgaggc tgcggtggcg
    59761 agttgcgttt ttaactcacc gtagagtggt gcgaaacgac ggttaaaacc gaccatcaac
    59821 gtcagttttt tccgcgccgc cagttccacc agcctttcag catcgcgcag attttctgcc
    59881 agcggtttat cgacacagac atgtaccccc gcattgagta acgtactgac cacgtcaaag
    59941 tggctggcgg tgctggagtg cacaaaaacc gcatcacaac tggcggcaag gctggataac
    60001 gaatcggcat aaggaatgcg ccagctgtca caaattggca gggctttggc gcgcgtaggc
    60061 gaccaggctc cttgtaacgt ccagtcagac gctgccgcca gcaccggtaa ccacgctttt
    60121 tgcgcaatgc cgcctaatcc cactacgccg atacgtaatt ttttcacttt aatctcccag
    60181 atgggccagc aacgaatcaa gacgttgttt cagttctgcc acttcgattt ccagggcttc
    60241 gacgcgggtc tataaatcac cgtcaaccat gttcgacata gccgttaccg ccggctgatc
    60301 ttcaacctca ccactgaaaa gatgcatgta gcggctttcg cgtttacccg gttcgcgggc
    60361 cagacgcacc acaaaaggac catcttcgcg attcgccagt tgttccagcg tcgactccac
    60421 ttccgccata tcgctgaatt catacattcg cgcggcgcgg ctgcgcagtt cacccggcgt
    60481 ctgggcacca cgcaataaca acgtggtgat taacgccact tctgcggcgc tcagtttcag
    60541 atcgccaaat tctgaattac aaaaacgttg ctcatatttg gtgacccgat taccaaaacc
    60601 gctcactgtg cgtagataat gacgtttgac cagattatcc agctgttcct gcacttcgga
    60661 ttcactcaga ttcatgaccg gtttacggtt cgttttctga ttacaggccg tgactacacc
    60721 attgactgag agcgggtatt gctccggcgt ggtcacctgc ttttccagca agcaaccaat
    60781 tacgcgagct tccaaggcag taagttgata tttcatgaga ttctccttag cggccgggcg
    60841 tccagtctgg ggtagttaat gccgtcagta cgtgatcgcg ccattgtcca tcaatcaaca
    60901 gatagtcttt cgcatagcct tctttttcaa aacccagtcg cgccagtaaa tcaccgctgc
    60961 gtttattgtg cggcatataa ttagccataa tgcgatgaat atgttgggtg cgctgcatat
    61021 aacgaatggc tgcggtcagg gcttcaaaca tgagtccttt gccctgccat ttttgcccaa
    61081 tcgaataacc gagatagcag gcatgaaaag agccgcgaac aacattggaa aaattggcaa
    61141 cgccaataat ctctttttcg tccgggtcga ataagccaaa gtagaaagct gaaccttgtt
    61201 tatgaaattc gttaatcatc cctagcctgg cctgccagcc tgatggataa cagtggcttt
    61261 cgtcgcgcac tggctcccag ggcttgagga aatggcgatt ctctgcgtaa taatccgcca
    61321 gacgccaggc atcacgatca tgcaccagac gcacgaccag tcggtctgtg gttaagcgca
    61381 cttttggcac gttactgcga tagccaaaca ttctatacct actccttccc gtaacgtctt
    61441 taatacgctg taagtattac tatacctgcg caaccagacc ctgtgaaaag tccaaaatgc
    61501 cattttctgc attattcaca tatttgataa aaactctcta tcaaagcgac gttttgataa
    61561 aaaaatattg tccccgaagt gatgggaaaa gtcttagcgg caatgcagaa tagaaagtgc
    61621 tcatctttta tttcccggga ggggaaatgt cccgcgtatc gcaggcgagg aacctgggta
    61681 aatatttcct gctcatcgat aatatgctgg tcgtgctggg gttctttgtt gtcttcccgc
    61741 tgatctctat ccgcttcgtt gatcaaatgg gctgggccgc cgtcatggtc ggtattgctc
    61801 tcggtctacg ccagtttatt cagcaaggtc tgggtatttt cggcggcgca attgccgacc
    61861 gctttggtgc caaaccgatg attgttaccg gtatgctgat gcgcgccgcc ggattcgcca
    61921 caatgggtat cgcccacgaa ccgtggctat tgtggttttc atgcctgctc tcgggactcg
    61981 gcggcacgtt gtttgatccg ccgcgttcgg cgctggtggt gaaactaatc cgtccacaac
    62041 aacgtggtcg ttttttctcg ctgttgatga tgcaggacag tgccagtgcg gtcattggcg
    62101 cattgttggg gagctggctg ttgcaatacg actttcgcct ggtctgcgcc acaggggccg
    62161 ttttgtttgt gctatgtgcg gcgttcaatg cgtggttgtt accggcatgg aaactctcca
    62221 ccgtacgcac gcccgttcgc gaaggcatga cccgcgtgat gcgtgacaag cgttttgtca
    62281 cctatgtcct gacgctggcg ggttactaca tgctggctgt acaagtgatg ctgatgctgc
    62341 caattatggt caacgacgtg gctggcgcgc cctctgccgt taaatggatg tatgccattg
    62401 aagcgtgtct gtcgttaacg ttgctctacc ctatcgcccg ctggagtgaa aagcattttc
    62461 gtctggaaca ccggttgatg gctgggctgt tgataatgtc attaagcatg atgccggtgg
    62521 gcatggtcag cggcctacaa caacttttca ccctgatttg tctgttttat atcgggtcga
    62581 tcattgccga gcctgcgcgt gaaaccttaa gtgcttcgct ggcggacgca agagctcgcg
    62641 gcagctatat ggggtttagc cgtctgggtc tggcgattgg cggcgctatt ggttatatcg
    62701 gtggtggctg gctgtttgac ctgggcaaat cggcgcacca gccagagctt ccgtggatga
    62761 tgctgggaat tattggcatc ttcactttcc ttgcgctggg ttggcagttt agtcagaaac
    62821 gcgccgcgcg tcgtttgctg gaacgcgacg cctgatttga tactcccccc tgactcctcc
    62881 atactgaaag tgaactgaca gccaaatgga ggagtcacgt gaagctatac atttacgatc
    62941 actgccctta ctgcctcaaa gcccgcatga ttttcggcct gaaaaatatc cccgtcgaat
    63001 tacatgttct gctcaacgac gacgcagaaa cacccacccg gatggtcggt caaaaacagg
    63061 ttcccattct gcaaaaagat gacagccgct atatgccaga aagcatggat atcgttcact
    63121 atgtcgataa actcgacggc aaaccgttac tgaccggcaa acgttcccct gccattgaag
    63181 agtggctgcg caaggtcaat ggctacgcca acaaactgct gttgccgcgt tttgccaaat
    63241 cggcatttga tgagttttct actcccgccg cgcgcaaata tttcgtcgac aagaaagagg
    63301 ccagcgcggg taattttgcc gacctgctgg cccactctga cggtctgatt aagaatatca
    63361 gcgatgattt gcgtgcgctg gacaaactga tcgtcaaacc gaacgccgtg aatggcgaac
    63421 tttcggaaga tgatattcag ctatttccgc tactgcgtaa tctgacgctg gtagccggaa
    63481 tcaactggcc aagccgcgtt gctaattacc gcgataatat ggcgaaacag acacaaatca
    63541 atttgttatc atcaatggcg atttaatcct gacccgccgc ggcagcctgt attcgcgggt
    63601 tgccgccgtt ttcagttttt tctgctggcg agtttatcgc tggtgacaca tgctgcgttg
    63661 tgtcaggata ttgacgcaga tttcatgtcg aggactacca tgaacaagtt tttatttgcc
    63721 gctgcattga tcgtcagtgg cctgctcgtt ggctgtaatc aactcaccca atacaccatc
    63781 accgaacaag aaattaacca gtcgcttgcg aaacataata atttctcaaa agatatcggt
    63841 ttacccggcg tggctgacgc ccatattgtt ctgacaaact tgaccagcca aattggtcgc
    63901 gaagagccga ataaggttac cctaaccgga gacgccaatc tggacatgaa ctccctgttc
    63961 ggtagtcaga aagcgaccat gaaactgaag ctgaaagcgc tgcccgtgtt tgataaagaa
    64021 aaaggtgcga tcttcctgaa agagatggaa gtggtcgatg cgacggtaca accggaaaaa
    64081 atgcaaacgg tgatgcaaac gttgcttccc tatttgaacc aggcattacg caattacttt
    64141 aaccagcaac ctgcttacgt cctgcgcgaa gatggcagtc agggcgaagc aatggcgaaa
    64201 aaactggcga aaggcattga agtgaagcca ggcgaaattg tcattccgtt tacggattaa
    64261 tgacgagggc gcattcgcgc cctttatttt tcgtgcaaag gaaaacgttt ccgcttatcc
    64321 tttgtgtccg gcaaaaacat cccttcagcc ggagcataga gattaatgac tgcaccatcc
    64381 caggtattaa agatccgccg cccagacgac tggcaccttc acctccgcga tggcgacatg
    64441 ttaaaaactg tcgtgccgta taccagcgaa atttatggac gggctatcgt aatgcccaat
    64501 ctggctccgc ccgtgaccac agttgaggct gccgtggcgt atcgccaacg tattcttgac
    64561 gccgtacctg ccgggcacga tttcacccca ttgatgacct gttatttaac agattcgctg
    64621 gatcctaatg agctggagcg cggatttaat gaagccgtgt tcaccgctgc aaaactttac
    64681 ccggcaaacg caaccactaa ctccagccac ggcgtgacgt caattgacgc aatcatgccg
    64741 gtacttgagc gcatggaaaa aatcggtatg ccgctactgg tgcatggtga agtgacacat
    64801 gcagatatcg acatttttga tcgtgaagcg cgctttatag aaagcgtgat ggaacctctg
    64861 cgccagcgcc tgactgcgct gaaagtcgtt tttgagcaca tcaccaccaa agatgctgcc
    64921 gactatgtcc gtgacggaaa tgaacggctg gctgccacca tcactccgca gcatctgatg
    64981 tttaaccgca accatatgct ggttggtggc gtgcgtccgc acctgtattg tttacccatc
    65041 ctcaaacgca atattcacca acaggcattg cgtgaactgg tcgccagcgg ttttaatcga
    65101 gtattcctcg gtacggattc tgcgccacat gcacgtcatc gcaaagagag cagttgcggc
    65161 tgcgcgggct gcttcaacgc cccaaccgcg ctgggcagtt acgctaccgt ctttgaagag
    65221 atgaatgctc tgcagcactt tgaagcattc tgttctgtaa acggcccgca gttctatggc
    65281 ttgccggtca acgacacatt catcgaactg gtacgtgaag agcaacaggt tgctgaaagc
    65341 atcgcactga ctgatgacac cctggtgcca ttcctcgccg gggaaacggt acgctggtcc
    65401 gttaaacaat aaaaatcgta gcttcctgtt gtcattaggt tattttacct gtataaataa
    65461 ccagtatgtt caacaggggg ctattatgcg aattgaagtc accatagcga aaacttctcc
    65521 attgccagct ggggctattg acgccctggc tggcgaactt tcccgccgta ttcagtatgc
    65581 gtttcctgat aatgaaggcc acgtatcggt acgttatgcc gcagcgaata atttatcggt
    65641 tattggcgca acaaaagaag ataaacagcg cattagcgaa attctccagg aaacgtggga
    65701 aagcgccgat gactggtttg tcagcgaata atatgcagtg atttttgttt ttgccggatc
    65761 gccccggctt ttttatttta cttacgaatt ttgagtacaa aatttctaca cttcctcaaa
    65821 ataattgcac aatatgctaa aaaaatatga atgacatgct gttttattgt tcatataaat
    65881 cttaaaagca gaaatttatc tgttcccccc tttaaaaatt cggtgaatac ccttacttat
    65941 tggtatactg aagttgcttc agataaaaac gcattgaacc tcgaataacg ttgtctagta
    66001 acacgaatta gggggccatg atggaaaaaa ataatgaagt cattcagact catccgctcg
    66061 tagggtggga catcagcacc gttgatagct atgatgcgct gatgttgcgt ttgcactacc
    66121 agaccccaaa taagtccgag caggaaggga ctgaagttgg tcagacgctc tggttaacca
    66181 ctgatgttgc cagacaattt atttcgatat tagaagcagg aatcgccaaa attgaatccg
    66241 gtgatttccc ggtaaacgag tatcggcgtc attaacatga catgaccatc cacccaaaag
    66301 gcacctcacc ggtgccttta ccatttctgg gttgtatatc tgcattcagt taattactct
    66361 gcttaaacca tgagagcaga gagaaaaaaa tgaaatacga tctcatcatt attggcagcg
    66421 gttccgtagg cgctgccgcc gggtattatg caacccgcgc cggtttaaac gtgctaatga
    66481 ccgacgccca tatgccaccg catcaacacg gcagccacca cggcgatacg cgattaattc
    66541 gccatgctta tggtgaaggc gaaaagtatg tcccgttggt cctccgcgcg caaacgctgt
    66601 gggatgaact ttcccgccac aacgaagatg atcccatttt tgtacgctct ggtgtcatta
    66661 acctcggccc ggctgactcc gcatttctcg ccaacgtcgc ccacagcgcc gaacagtggc
    66721 aactcaacgt tgaaaagctc gatgcgcaag ggattatggc ccgctggcca gaaatacgcg
    66781 tcccggacaa ctacatcggc ttatttgaga ctgattccgg ttttttgcgc agcgaactgg
    66841 cgattaaaac ctggatccaa ctggcgaagg aagcgggctg tgcgcaactg ttcaactgcc
    66901 cggtcaccgc aattcgtcat gacgatgatg gcgtaactat tgaaacggtt gacggtgagt
    66961 atcaggcgaa aaaagcgatt gtctgcgcgg gaacatgggt aaaagacctg ctcccggagc
    67021 tgcctgtcca gcccgtacgt aaagtatttg cctggtatca ggccgatggc cgctatagcg
    67081 tgaagaataa attcccggcg tttaccggtg aactgcccaa tggcgatcaa tattatggtt
    67141 ttccggcaga aaacgacgcg ttgaagattg gcaaacataa cggaggccag gttatccatt
    67201 cagcggatga acgtgttccg tttgcggaag tggccagtga tgggtcagaa gccttcccgt
    67261 tcttgcgcaa tgtattgccg ggtatcggtt gctgcctgta cggcgctgcc tgcacctatg
    67321 ataattcgcc tgacgaagat tttattatcg ataccctacc cggccacgat aatacactgc
    67381 tcattaccgg cctgagtggg cacggtttta aatttgcgtc agttttaggg gaaatagctg
    67441 ccgattttgc gcaagaccaa aaaagcgatt ttgatttgac gccattcagg ctttcccgct
    67501 tccaataatc atcattacgg cctccagaat gaggccgttt ttacaggagt atttaatgcg
    67561 tcggctgttg cactatctca tcaataatat tcgcgagcat ttgatgcttt atctttttct
    67621 ctggggattg ttggcaatta tggatctgat ctatgtgttt tatttttagt tttccgcact
    67681 gacataaact gacaaatatt taatctccct ttcatttagc gtgaaaaatt gaatgatatt
    67741 attcctgctt ctacagcact caccagacat ctctgatgct gaacccacta aacttatgaa
    67801 gattctttca ttattaattc aaaattattg aaataaaaat aaatatcatt caatttgtta
    67861 tttttgtgtt gcaattcatt ctccgacacg ccattttctt tctctcgcaa attcgtattg
    67921 gatgcactta tgtcattcac aaatacccct gaacgctatg gtgttatatc tgcggccttc
    67981 cattggctaa gcgcaattat tgtctacggc atgtttgctt taggtctgtg gatggtcacg
    68041 ctcagttatt acgatggctg gtatcacaaa gcacccgaac tgcataaaag catcggtatt
    68101 ttgttaatga tggggctggt tattcgcgtt ctgtggcgtg tcatatctcc accgccgggt
    68161 ccgctgccga gttattcgcc catgactcgt cttgccgcaa aagctggaca tctcgcattg
    68221 tacctattgc tgttcgccat cggcatcagc ggctatctga tctcaactgc cgatggtaaa
    68281 ccgatcagcg tttttggctg gtttgacgtc cccgcgaccc ttgccgacgc tggcgcacag
    68341 gcagactttg ccggtgccct gcatttttgg ctcgcgtgga gcgtcgtagt actgtctgtt
    68401 atgcacggat ttatggccct gaagcatcat ttcatcgata aagacgacac tctgaagcgc
    68461 atgctgggaa agtcgtcatc tgactatgga gtataaaaaa tgaaaaaaag cctgcttggt
    68521 ttaaccttcg cgtccctgat gttctctgcc ggttcagcgg ttgccgccga ttacaaaatt
    68581 gacaaagaag gtcagcacgc ctttgttaac ttccgcatac agcatcttgg ctatagctgg
    68641 ttatatggca cctttaaaga tttcgacggt acttttacct ttgatgaaaa aaatccggct
    68701 gccgataaag tgaatgtgac aattaacacc accagcgtcg atactaacca cgccgaacgc
    68761 gataaacatc ttcgcagtgc ggattttctc aataccacaa aatatccaca ggcaacattc
    68821 acctccacca gcgtgaagaa agacggtgac gaactggata ttaccggcga tctgacgctg
    68881 aatggcgtaa ccaaacccgt cacgctggaa gcgaaattaa ttggtcaggg cgacgaccca
    68941 tggggcggta aacgtgcagg ctttgaggcc gaaggcaaaa ttaagctcaa agacttcaat
    69001 atcaagacag atttaggtcc agcttctcag gaagtggatc tgattatttc agtggaaggc
    69061 gtacagcaga agtaagagtg aaatgttgcc ggatggcacg catccggcaa tgatatttat
    69121 tctgttggat caggaatacc cagtgttgta ttcagacgtc cacgagactt attaaagatc
    69181 ttattgccat tttcacgtcc tgcccgacgg cgtcgctgtt cttctggcgg taacgcgctt
    69241 tcttcgcagc aaatctcact acaacaacct ttgtattttt ccgcgcatac tggacactga
    69301 ataaacagca ggtggcagcc atcattttta cagttggtat ggctgtcgca cggcgcgccg
    69361 cactggtggc aatgcgcgat aatctcatcc gatatacgtt cgcccatccg ctcgtcaaaa
    69421 acaaaatttt tgccaataaa acgcaccggc aagccctgct cgcgcgcctt acgggcgtat
    69481 tcaataattc cgccctcgat atgccagact ttgttgaatc cgttatgttt catccaggca
    69541 ctggcctttt cacaacgaat gccgccggtg cagtacatga cgattttttt atctttatgt
    69601 gcctgcatca tctcgactgc ttttggcagc tgctcacgga aggtatctgc cggaatttcg
    69661 agcgcgtttt caaagtgccc cacttcatac tcatagtggt tacgcatgtc gataaatagt
    69721 gcatcgggat cgtcaagcat ggcgttcact tccgccgctt gcagatactc accaacattg
    69781 ctggcatcaa agtgaggatc gtcaataccg tcggcaacaa tgcgatcgcg taccttcatg
    69841 cgcagtaccc agaaggattt cccgtcatca tccaacgcga tattcaggcg taaaccctct
    69901 aaagccgggt cgaaggcata gagctgcgcg cgaaatgttt caacattgct cgcaggtacg
    69961 ctgatttgcg cgttaatgcc ctcatgcgcc agatacactc gcccaaaaac attcagcgcg
    70021 gtaaacagct gatataaagc gtcacgggtc gccttaggat cggcgatgtg gaaatacttg
    70081 taaaacgaaa tggtggttcg cggttcgctc tcagccaaca ttttggcttt taacgcgtcg
    70141 ttggaaatgc ggttgtgtaa cactggcatg gtgtacggtt cctgcgagat gggaaagtaa
    70201 aaatccgcgg catgatatag caattatcga taattaacat ccacacattt tacgctacat
    70261 ttgcgcatta aaaattattt gttatttaca agcgcggcaa tttcgcccag tcttcagcca
    70321 caaattttgg ttgcgggcga aaaaatgcga caatacatac aattgcccgt ataggttgaa
    70381 aaacaggatt gatatgacga atctacccaa gttctccacc gcactgcttc atccgcgtta
    70441 ttggttaacc tggttgggta ttggcgtact ttggttggtc gtgcaattgc cctacccgat
    70501 tatctaccgc ctcggttgtg gattaggaaa actggcgtta cgttttatga aacgacgcgc
    70561 aaaaattgtg catcgcaacc tggaactgtg cttcccggaa atgagcgaac aagaacgccg
    70621 taaaatggtg gtgaagaatt tcgaatccgt tggcatgggc ctgatggaaa ccggtatggc
    70681 gtggttctgg ccggaccgcc gaatcgcccg ctggacggaa gtgatcggca tggaacacat
    70741 tcgtgacgtg caggcgcaaa aacgcggcat cctgttagtt ggcatccatt ttctgacact
    70801 ggagctgggc gcgcggcagt ttggtatgca ggaaccgggg attggcgttt accgcccgaa
    70861 cgataatcca ctgattgact ggctacaaac ctggggccgt ttgcgctcaa ataaatcgat
    70921 gctcgaccgc aaagatttaa aaggcatgat taaagccctg aaaaaaggcg aagtggtctg
    70981 gtacgcacca gatcatgatt acggcccgcg ctcaagcgtt ttcgtcccgt tatttgccgt
    71041 tgagcaggct gcgaccacga ccggaacctg gatgctggca cggatgtccg gcgcatgtct
    71101 ggtgcccttc gttccacgcc gtaagccaga tggcaaaggg tatcaattga ttatgctgcc
    71161 accagagtgt tctccgccac tggatgatgc cgaaactacc gccgcgtgga tgaacaaagt
    71221 ggtcgaaaaa tgcatcatga tggcaccaga gcagtatatg tggttacacc gtcgctttaa
    71281 aacacgcccg gaaggcgttc cttcacgcta ttaaatctcc catgccggat gcttcagaat
    71341 ggcatccggc attaccacag caaatccccc tgatttagcg ataaaagctc tctggattgc
    71401 gccccctgga agtcgggcgc ataattagtg tgtttatctt ttcttcttat gttcaccgcg
    71461 cctggcgcac caacagcgga ttgctatgtc accctgtgaa aatgacaccc ctataaactg
    71521 gaaacgaaac ctgatcgtcg cctggctagg ctgttttctt accggggccg acttcagtct
    71581 ggtaatgccc ttcttacccc tctacgttga gcagcttggt gttaccggcc actccgccct
    71641 gaatatgtgg tccggtattg tcttcagtat aatgggttcc taaacacatc attttaggta
    71701 ttgaatatga gacgggcaat cagctacatc agattttcaa gtgaacgaca actgaaaggg
    71761 gactctgtac gccgacaatc taagctggtt actgactggc ttgataagaa ccctgggttt
    71821 tacttggatt catccttgag ctttaaagat ttggggaaaa gtgcttttag tggtaagcac
    71881 ctgaaaggtg gtctaggtga ctttttaacc gctatcgaga aagggcttgt taaagctggt
    71941 gatacgctac taatcgagag tttagaccgt ctgtcacgtc aggatattga tattgcttct
    72001 gagctattac gccgtatctt aagggctggt gtagatgtag tcacactgag cgatggagaa
    72061 cattacacac gagaaagttt aaaagacccg ttagccctga ttaaaagcat tctgataatg
    72121 cagcgggcgc atgaggaaag tttgagaaag tctgagcgtg tccaagctgc atggaaccgt
    72181 aaaaaagaac tcatctccga aggtatcaag gtatc
//
LOCUS       DAEEUE010000006        70704 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.006, whole genome shotgun sequence.
ACCESSION   DAEEUE010000006 DAEEUE010000000
VERSION     DAEEUE010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70704
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.006"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..795
                     /locus_tag="KLK84_000421"
     CDS             <1..795
                     /locus_tag="KLK84_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBE2007886.1"
                     /translation="WLPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLD
                     DDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAF
                     ALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQ
                     FVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILY
                     LSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            806..1897
                     /locus_tag="KLK84_000422"
     CDS             806..1897
                     /locus_tag="KLK84_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE2007887.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTAAITVMVR"
     gene            complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="KLK84_000423"
     CDS             complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="KLK84_000423"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBE2007888.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2865..4901
                     /gene="lpoA"
                     /locus_tag="KLK84_000424"
     CDS             2865..4901
                     /gene="lpoA"
                     /locus_tag="KLK84_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HBE2007889.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4859..5254
                     /locus_tag="KLK84_000425"
     CDS             4859..5254
                     /locus_tag="KLK84_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBE2007890.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5274..5864
                     /gene="diaA"
                     /locus_tag="KLK84_000426"
     CDS             5274..5864
                     /gene="diaA"
                     /locus_tag="KLK84_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HBE2007891.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5874..6449
                     /gene="yraP"
                     /locus_tag="KLK84_000427"
     CDS             5874..6449
                     /gene="yraP"
                     /locus_tag="KLK84_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HBE2007892.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6563..7603)
                     /locus_tag="KLK84_000428"
     CDS             complement(6563..7603)
                     /locus_tag="KLK84_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBE2007893.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMALLIATLVQKWVRETLQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVCILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7676..8311)
                     /locus_tag="KLK84_000429"
     CDS             complement(7676..8311)
                     /locus_tag="KLK84_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBE2007894.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8439..8957
                     /gene="yhbO"
                     /locus_tag="KLK84_000430"
     CDS             8439..8957
                     /gene="yhbO"
                     /locus_tag="KLK84_000430"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HBE2007895.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8937..9380)
                     /locus_tag="KLK84_000431"
     CDS             complement(8937..9380)
                     /locus_tag="KLK84_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HBE2007896.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9431..9733
                     /gene="yhbQ"
                     /locus_tag="KLK84_000432"
     CDS             9431..9733
                     /gene="yhbQ"
                     /locus_tag="KLK84_000432"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HBE2007897.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9720..10223)
                     /locus_tag="KLK84_000433"
     CDS             complement(9720..10223)
                     /locus_tag="KLK84_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBE2007898.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10217..10741)
                     /locus_tag="KLK84_000434"
     CDS             complement(10217..10741)
                     /locus_tag="KLK84_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HBE2007899.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10950..11945
                     /locus_tag="KLK84_000435"
     CDS             10950..11945
                     /locus_tag="KLK84_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBE2007900.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTSLGAYHRKWQ"
     gene            11954..12832
                     /locus_tag="KLK84_000436"
     CDS             11954..12832
                     /locus_tag="KLK84_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBE2007901.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12913..13920
                     /locus_tag="KLK84_000437"
     CDS             12913..13920
                     /locus_tag="KLK84_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HBE2007902.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="KLK84_000438"
     CDS             complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="KLK84_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HBE2007903.1"
                     /translation="MATRTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="KLK84_000439"
     CDS             complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="KLK84_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HBE2007904.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="KLK84_000440"
     CDS             complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="KLK84_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HBE2007905.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="KLK84_000441"
     CDS             complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="KLK84_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HBE2007906.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="KLK84_000442"
     CDS             complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="KLK84_000442"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBE2007907.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="KLK84_000443"
     CDS             complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="KLK84_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBE2007908.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21298..22242)
                     /gene="truB"
                     /locus_tag="KLK84_000444"
     CDS             complement(21298..22242)
                     /gene="truB"
                     /locus_tag="KLK84_000444"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBE2007909.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="KLK84_000445"
     CDS             complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="KLK84_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBE2007910.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22807..25455)
                     /gene="infB"
                     /locus_tag="KLK84_000446"
     CDS             complement(22807..25455)
                     /gene="infB"
                     /locus_tag="KLK84_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBE2007911.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="KLK84_000447"
     CDS             complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="KLK84_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBE2007912.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="KLK84_000448"
     CDS             complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="KLK84_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBE2007913.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27654..27730)
                     /locus_tag="KLK84_000449"
     tRNA            complement(27654..27730)
                     /locus_tag="KLK84_000449"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27694..27696),aa:Met,seq:cat)
     gene            28078..29421
                     /gene="argG"
                     /locus_tag="KLK84_000450"
     CDS             28078..29421
                     /gene="argG"
                     /locus_tag="KLK84_000450"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBE2007914.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29429..31054)
                     /locus_tag="KLK84_000451"
                     /pseudo
     CDS             complement(29429..31054)
                     /locus_tag="KLK84_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245267.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31513..31599)
                     /locus_tag="KLK84_000452"
     tRNA            complement(31513..31599)
                     /locus_tag="KLK84_000452"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31563..31565),aa:Leu,seq:gag)
     gene            complement(31614..31946)
                     /gene="secG"
                     /locus_tag="KLK84_000453"
     CDS             complement(31614..31946)
                     /gene="secG"
                     /locus_tag="KLK84_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBE2007915.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32174..33511)
                     /gene="glmM"
                     /locus_tag="KLK84_000454"
     CDS             complement(32174..33511)
                     /gene="glmM"
                     /locus_tag="KLK84_000454"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBE2007916.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33504..34352)
                     /gene="folP"
                     /locus_tag="KLK84_000455"
     CDS             complement(33504..34352)
                     /gene="folP"
                     /locus_tag="KLK84_000455"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBE2007917.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34442..36376)
                     /gene="ftsH"
                     /locus_tag="KLK84_000456"
     CDS             complement(34442..36376)
                     /gene="ftsH"
                     /locus_tag="KLK84_000456"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBE2007918.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36476..37105)
                     /gene="rlmE"
                     /locus_tag="KLK84_000457"
     CDS             complement(36476..37105)
                     /gene="rlmE"
                     /locus_tag="KLK84_000457"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HBE2007919.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37231..37524
                     /gene="yhbY"
                     /locus_tag="KLK84_000458"
     CDS             37231..37524
                     /gene="yhbY"
                     /locus_tag="KLK84_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBE2007920.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37680..38156)
                     /gene="greA"
                     /locus_tag="KLK84_000459"
     CDS             complement(37680..38156)
                     /gene="greA"
                     /locus_tag="KLK84_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBE2007921.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38404..39837
                     /gene="dacB"
                     /locus_tag="KLK84_000460"
     CDS             38404..39837
                     /gene="dacB"
                     /locus_tag="KLK84_000460"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBE2007922.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(40023..41195)
                     /gene="cgtA"
                     /locus_tag="KLK84_000461"
     CDS             complement(40023..41195)
                     /gene="cgtA"
                     /locus_tag="KLK84_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HBE2007923.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKVIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41211..42176)
                     /locus_tag="KLK84_000462"
     CDS             complement(41211..42176)
                     /locus_tag="KLK84_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBE2007924.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42303..42560)
                     /gene="rpmA"
                     /locus_tag="KLK84_000463"
     CDS             complement(42303..42560)
                     /gene="rpmA"
                     /locus_tag="KLK84_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBE2007925.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42581..42892)
                     /gene="rplU"
                     /locus_tag="KLK84_000464"
     CDS             complement(42581..42892)
                     /gene="rplU"
                     /locus_tag="KLK84_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBE2007926.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43150..44121
                     /gene="ispB"
                     /locus_tag="KLK84_000465"
     CDS             43150..44121
                     /gene="ispB"
                     /locus_tag="KLK84_000465"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HBE2007927.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44348..44626
                     /gene="sfsB"
                     /locus_tag="KLK84_000466"
     CDS             44348..44626
                     /gene="sfsB"
                     /locus_tag="KLK84_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HBE2007928.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44674..45933)
                     /gene="murA"
                     /locus_tag="KLK84_000467"
     CDS             complement(44674..45933)
                     /gene="murA"
                     /locus_tag="KLK84_000467"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBE2007929.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45988..46242)
                     /gene="ibaG"
                     /locus_tag="KLK84_000468"
     CDS             complement(45988..46242)
                     /gene="ibaG"
                     /locus_tag="KLK84_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HBE2007930.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46402..46695)
                     /gene="mlaB"
                     /locus_tag="KLK84_000469"
     CDS             complement(46402..46695)
                     /gene="mlaB"
                     /locus_tag="KLK84_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HBE2007931.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46695..47330)
                     /gene="mlaC"
                     /locus_tag="KLK84_000470"
     CDS             complement(46695..47330)
                     /gene="mlaC"
                     /locus_tag="KLK84_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HBE2007932.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47349..47900)
                     /gene="mlaD"
                     /locus_tag="KLK84_000471"
     CDS             complement(47349..47900)
                     /gene="mlaD"
                     /locus_tag="KLK84_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HBE2007933.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47905..48687)
                     /gene="mlaE"
                     /locus_tag="KLK84_000472"
     CDS             complement(47905..48687)
                     /gene="mlaE"
                     /locus_tag="KLK84_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HBE2007934.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48695..49504)
                     /gene="mlaF"
                     /locus_tag="KLK84_000473"
     CDS             complement(48695..49504)
                     /gene="mlaF"
                     /locus_tag="KLK84_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HBE2007935.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49714..50679
                     /locus_tag="KLK84_000474"
     CDS             49714..50679
                     /locus_tag="KLK84_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBE2007936.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50693..51679
                     /gene="kdsD"
                     /locus_tag="KLK84_000475"
     CDS             50693..51679
                     /gene="kdsD"
                     /locus_tag="KLK84_000475"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HBE2007937.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51700..52266
                     /gene="kdsC"
                     /locus_tag="KLK84_000476"
     CDS             51700..52266
                     /gene="kdsC"
                     /locus_tag="KLK84_000476"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HBE2007938.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52263..52838
                     /gene="lptC"
                     /locus_tag="KLK84_000477"
     CDS             52263..52838
                     /gene="lptC"
                     /locus_tag="KLK84_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HBE2007939.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52807..53364
                     /gene="lptA"
                     /locus_tag="KLK84_000478"
     CDS             52807..53364
                     /gene="lptA"
                     /locus_tag="KLK84_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HBE2007940.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53371..54096
                     /gene="lptB"
                     /locus_tag="KLK84_000479"
     CDS             53371..54096
                     /gene="lptB"
                     /locus_tag="KLK84_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HBE2007941.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLISH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54144..55577
                     /gene="rpoN"
                     /locus_tag="KLK84_000480"
     CDS             54144..55577
                     /gene="rpoN"
                     /locus_tag="KLK84_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBE2007942.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55600..55887
                     /gene="hpf"
                     /locus_tag="KLK84_000481"
     CDS             55600..55887
                     /gene="hpf"
                     /locus_tag="KLK84_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HBE2007943.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56005..56496
                     /gene="ptsN"
                     /locus_tag="KLK84_000482"
     CDS             56005..56496
                     /gene="ptsN"
                     /locus_tag="KLK84_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HBE2007944.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56542..57396
                     /gene="rapZ"
                     /locus_tag="KLK84_000483"
     CDS             56542..57396
                     /gene="rapZ"
                     /locus_tag="KLK84_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBE2007945.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57393..57665
                     /gene="npr"
                     /locus_tag="KLK84_000484"
     CDS             57393..57665
                     /gene="npr"
                     /locus_tag="KLK84_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HBE2007946.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57878..58504
                     /gene="yrbL"
                     /locus_tag="KLK84_000485"
     CDS             57878..58504
                     /gene="yrbL"
                     /locus_tag="KLK84_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HBE2007947.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGGDKEIRRE
                     LKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCRYE
                     EDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLIPL
                     ATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58501..59228)
                     /gene="mtgA"
                     /locus_tag="KLK84_000486"
                     /pseudo
     CDS             complement(58501..59228)
                     /gene="mtgA"
                     /locus_tag="KLK84_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59225..59878)
                     /gene="elbB"
                     /locus_tag="KLK84_000487"
     CDS             complement(59225..59878)
                     /gene="elbB"
                     /locus_tag="KLK84_000487"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HBE2007948.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGVGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60108..62444)
                     /gene="arcB"
                     /locus_tag="KLK84_000488"
     CDS             complement(60108..62444)
                     /gene="arcB"
                     /locus_tag="KLK84_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HBE2007949.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLELNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62540..63469)
                     /locus_tag="KLK84_000489"
     CDS             complement(62540..63469)
                     /locus_tag="KLK84_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBE2007950.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAVRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64144..68604
                     /gene="gltB"
                     /locus_tag="KLK84_000490"
     CDS             64144..68604
                     /gene="gltB"
                     /locus_tag="KLK84_000490"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HBE2007951.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68617..70035
                     /gene="gltD"
                     /locus_tag="KLK84_000491"
     CDS             68617..70035
                     /gene="gltD"
                     /locus_tag="KLK84_000491"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HBE2007952.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70219..70698
                     /locus_tag="KLK84_000492"
     CDS             70219..70698
                     /locus_tag="KLK84_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HBE2007953.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT        17313 a        19178 c        17549 g        16664 t
ORIGIN      
        1 tggttaccgc gtagcctggt ttcctatcag atgaccagcc agaaagatcg cccaacccaa
       61 catgaaatgc gtcttgatgg ctcactgctg gatgatggtc gcctgagcta tagcctggaa
      121 caaagtctgg atgacgataa caaccataac agtagcctga acgccagtta ccgttcacct
      181 tatggaacct tcagtgccgg atacagttac ggtaatgaca gcagccaata caattacggc
      241 gttaccggcg gcgtggttat ccatcctcat ggcgtgacgc tctcgcaata tctgggcaac
      301 gcttttgcgc ttatcgatgc taacggggct tctggcgtga ggatacaaaa ctatccgggg
      361 attgctactg atccctttgg ctatgcagtg gttccttatc tcacgactta tcaggaaaac
      421 cgtctctcgg tagatactac gcagctgccc gataacgtcg atcttgaaca aacaacacag
      481 tttgtggtgc ccaacagagg tgcaatggta gcggcgcgtt tcaacgccaa tatcggttat
      541 cgcgtacttg ttacagtcag cgatcgcaac ggtaaaccgt tgccctttgg cgctcttgcc
      601 agcaacgatg atacggggca acaaagtatc gtcgatgagg gcggcatact atatctctct
      661 gggatatcga gtaaatcaca aagctggact gtacgctggg gaaatcaggc agatcaacaa
      721 tgtcagtttg cttttagtac accggattca gaaccaacaa cctctgtatt acaaggcaca
      781 gcgcagtgcc attaaggata aaaaaatgaa aagagcgcct cttataacag gacttttgtt
      841 gatatccaca tcctgcgctt atgcctcctc aggagggtgt ggagccgaca gcactagcgg
      901 tgcgacaaat tacagcagtg tggttgatga tgttacggtg aaccagacag ataacgtgac
      961 aggacgggag tttacctctg caacgctaag tagcactaac tggcaatacg cctgttcctg
     1021 ctctgcgggt aaggcagtta aacttgtcta tatggtcagc cccgtactta ccaccactgg
     1081 acatcagaca ggatattaca aactcaatga cagcctggat attaaaacca cattacaggc
     1141 aaacgacatt ccaggactca caaccgacca ggttgtctct gttaacaccc gattcacaca
     1201 gataaaaagc agcaccgtat attctgctgc aacccaaacg ggtgtttgcc agggtgacac
     1261 gtctcgttat ggacccgtta atattggtgc aaataccacc tttaccctgt atgtcaccaa
     1321 gccatttctc ggctcgatga ccattccgaa aacggatatt gccgtcatta aaggcgcgtg
     1381 ggtcgatgga atgggaagcc cgtctacagg tgacttccat gatttagtca agttatcgat
     1441 tcagggaaat ctcaccgccc cacagtcgtg caaaattaat cagggcgatg ttattaaagt
     1501 taattttgga ttcatcaatg gtcagaagtt taccacccgc aatgccatgc cagacggttt
     1561 tactccagta gactttgata tcacttatga ctgtggtgat acttcaaaga ttaaaaactc
     1621 gttgcaaatg cgcatcgacg gtacaactgg ggtagtagac cagtacaacc tggtcgccag
     1681 gcgaagaagt tcagacaatg tgcccgatgt cggtattcgt attgaaaatc tcggcggcgg
     1741 agttgcaaat attccttttc agaacggtat ccttcccgtt gatccttccg ggcatggcac
     1801 cgtcaatatg cgcgcctggc cagttaatct ggtcggtggt gagctggaaa caggaaaatt
     1861 tcagggcaca gccgccatta ccgtcatggt gcggtaaaag aagacgttac gttttgatta
     1921 acgcaacgct tcggcactgt tacccctgct gctccagcgc atacttatac agcgcatttt
     1981 tcttcacgcc atgaatttct gcggccagcg ccgccgcttt tttcagcggc agttctgcct
     2041 gtagcagcgc cagcgtgcgc agggcatcgg cgggtaagtc ttcttcctgt gctttatgac
     2101 cttcgacaat cagcaccatt tcgcctttgc gacggttttc atcttccttt acccacgcca
     2161 gcagctcgcc aacgggcgcg ccgtgaatgg tttcccaggt tttggtcagc tcacgcgcca
     2221 gaaccacgta gcgggattcg cctaataccg caacgatatc ttccaggcta tctaacagac
     2281 ggtgggtaga ttcataaaaa atcagcgtgc gcggctccgc ttcaatggct tttagcgcat
     2341 cacggcggcc ttttgatttg gcaggtaaaa agccttcgta acagaaacgg tcagatggta
     2401 aacccgcggc gcttaacgca gtgatagcag cacacggtcc gggtagcggc accacacgaa
     2461 tccccgcttc acggcaggta cgcaccagat ggtagccagg atcgttaatt agcggcgttc
     2521 cggcatcgga aaccagcgca atgttttgcc cctcttgcag ctttgccagc agcgtttcgg
     2581 ctttttgttg ttcgttatgg tcgtgcagcg caaacaaccg ggcattaatc ccaaaatgtt
     2641 gcagcaataa accggtgtga cgagtatcct cggcggcaat cagatcaacg gcctgtaata
     2701 cctctaacgt acgctgggtg atatccgcca gattgccgat tggcgtcggt acaatgtaaa
     2761 gctggccctg agaattatcc gccgattggt gttgtttcat tgtgtcgtcc gtattgccga
     2821 tttaatattg agcattgcgt aaaaaaaata tcactggata cattatggta ccctcaacat
     2881 tttctcgttt gaaagccgcg cgttgtctgc ctgttgttct ggcagccctg attttcgccg
     2941 gttgtggcac ccatactccc gatcagtcca ctgcttatat gcagggcacg gcgcaggctg
     3001 attctgcctt ttatcttcag cagatgcagc aaagctctga tgataccagg atcaactggc
     3061 aattactcgc cattcgtgca ctggtgaaag aaggtaaaac cgggcaggcg gttgagttgt
     3121 ttaaccaact accgcaagaa ctgaacgatt ctcagcgtcg cgagaaaaca ctgctggcgg
     3181 cagagattaa actggcgcag aaagattttg ctggcgcgca aaacttgctg gcgaaaatca
     3241 cacctgccga tttagaacaa aaccagcaag cgcgttactg gcaggcaaaa atcgatgcca
     3301 gccaggggcg tccttccatt gatttactgc gcgcgttaat tgctcaggaa ccgctgcttg
     3361 gcgcgaaaga aaaacagcag aatattgatg ccacctggca ggcgctctcc tccatgactc
     3421 aggaacaggc gaatacgctg gtgatcaacg ccgacgaaaa tattctgcaa ggctggctgg
     3481 atctgcagcg cgtctggttt gataaccgta acgatcccga catgatgaaa gccgggatcg
     3541 ccgactggca gaaacgttat ccgaacaatc cgggcgcgaa aatgctgcca acgcagttgg
     3601 ttaacgtaaa agcgtttaaa ccagcctcga ccaacaaaat cgccctgctg ttgccgctga
     3661 atggccaggc agcggtattt ggtcgcacta ttcagcaagg ctttgaagcc gcgaaaaata
     3721 tcggcactca gccagtggcg gctcaggtag ctgccgcacc tgccgcagac gtagcagaac
     3781 aacctcagcc gcaaactgcg gatagcgttg ccagcccggc acaagcctcg gttagcgatc
     3841 tgaccggtga tcagcctgcg gcccagccgg tgcctgtaag cgccccggcg acaagcaccg
     3901 cagcggtaag cgcacccgca aatccatccg cagagctgaa aatctacgat acctcatcac
     3961 aaccacttag ccagatctta agccaggttc agcaggatgg cgcgagtatt gtggttggtc
     4021 cgttgctgaa aaataacgtt gaagagttgc tgaagagcaa caccccgctg aacgtactgg
     4081 cactgaacca gccggagaat atcgaaaacc gcgtcaatat ttgttacttc gcgctttcac
     4141 cggaagacga agcgcgcgat gcagcgcgtc atattcgtga ccagggtaaa caagcgccgc
     4201 tggtgctgat cccacgcagt gcattgggcg atcgcgtagc caatgcgttt gcgcaagagt
     4261 ggcagaaact gggcggcggc accgttctgc aacaaaaatt tggttccacc agcgaattac
     4321 gcgcgggtgt taacggcggt tctggtattg ctttaacggg tagcccgatt actcccagag
     4381 cgacaaccga ctccggcatg acgaccaaca atccaacgct gcaaaccacg ccaaccgatg
     4441 accagttcac caataatggc ggtcgtgtcg atgcggtcta cattgtggca acgccgggtg
     4501 aaatcgcttt tatcaaaccg atgatcgcca tgcgtaacgg tagccagagc ggtgcaacgc
     4561 tgtacgccag ttcccgcagc gcacaaggca ccgcaggccc ggatttccga ctggagatgg
     4621 aaggcttgca gtacagcgaa atcccgatgc tggcaggcgg taatctgccg ttaatgcagc
     4681 aggcgctcag cgcggtgaac aacgattatt cgctggctcg catgtatgcg atgggcgttg
     4741 atgcctggtc gttggcaaat catttctcac aaatgcgcca ggttcagggt tttgaaatca
     4801 acggtaatac cggaagtctg acggctaacc cggattgcgt gattaacagg aagttatcat
     4861 ggctacagta ccaacaaggt caggtagtcc ccgccagtta accaccaaac agaccggcga
     4921 tgcgtgggaa gcacaagcgc gtcgctggct ggaaggcaaa ggactgcggt ttatcgccgc
     4981 taacgtgaac gagcgtggcg gcgagatcga tctgataatg cgtgaaggcc tgaccaccgt
     5041 ttttgtcgag gtacgctatc gccgctctgc gctttatggc ggcgcggcag ccagtgtgac
     5101 ccgcagcaaa caacacaaat tattacagac tgcccgcttg tggctcgcgc gtcataatgg
     5161 gagttttgat actgtggatt gccggttcga tgtggtagcc ttcaccggga atgaggttga
     5221 gtggattaag gatgccttta atgaccactc ataattaagg tttaaggatt agcgtgcaag
     5281 aaagaattaa agcttgcttc actgaaagca ttcaaactca aattgcggcg gcagaggcgc
     5341 ttccggatgc catctcccgt gcagccatga cgctggttca gtctctgctc aatggcaaca
     5401 aaatcctctg ttgtggtaat ggaacttccg ctgccaatgc acagcatttt gctgccagca
     5461 tgatcaaccg tttcgaaacg gagcggccca gcttacctgc cattgcacta aatactgata
     5521 atgttgtctt aacggcgatt gccaacgatc gcttacatga tgaagtgtat gcaaaacagg
     5581 tgcgggcgct gggtcatgcg ggagatgtat tgttagccat ttccacccgt ggcaacagcc
     5641 gcgatattgt taaagcagtt gaagccgccg ttacgcgtga tatgaccatt gtggcattga
     5701 ccggctatga cggcggcgaa cttgcaggtt tgttagggcc acaggatgtg gagatccgca
     5761 ttccttcgca tcgtagtgct cgcattcagg aaatgcatat gctgacggta aattgcctgt
     5821 gcgatctgat cgataacacg cttttccctc accaggatga ttaaggagaa tacatgaagg
     5881 cattatcgcc aatcgcagtc cttatttccg cgctgctgtt gcaaggttgt gttgccgctg
     5941 ccgtagtggg taccgctgct gtgggtacca aagccgcaac tgacccacgc agtgtcggca
     6001 cccaggtgga cgatggtacc ctggaagtgc gcgtgaacag cgcattgtcg aaagacgaac
     6061 agattaagaa agaaacccgt attaatgtca cggcctatca gggcaaagtg ctgctggttg
     6121 ggcagtcacc aaatgctgaa ctttcggctc gcgccaaaca gattgctatg ggcgtagacg
     6181 gtgccaacga agtgtataac gagattcgtc agggccagcc gattggtctg ggcgaagcat
     6241 ctaacgatac gtggatcacc accaaagtgc gttcgcagct cttaaccagc gacctggtga
     6301 aatcgtccaa cgtgaaagtg accaccgaaa acggtgaagt gttcctgatg gggctggtga
     6361 ctgaacgtga agcgaaagcg gcggcagata ttgccagccg ggtgagcggc gtaaagcggg
     6421 ttaccacagc gtttacgttt attaaatagc agccccttgt aatgcctgat gcgacgcttg
     6481 ccgcgtctta tcaggcctac aacgatgcaa accgtaggtc ggataaggcg tttacgccgc
     6541 atccgacatg ttttccctca aatcagaaca acagcgccag cccgccgaca atcacaccag
     6601 acactgccac catcgcccct gtcagccata aggctttcgc cgggaacgct ttgcgcagca
     6661 taatcagtga cggcaaactc actgccggga gcgtcatcaa caatgccagc gccggagcgg
     6721 ttcccatacc tgccagcatc atcgtttgta caatcggaat ttctgctgcc gtgggaatga
     6781 caaacaagca tcctgctacc gccatcgcca ccacccacat caggctgtta tcgacagcac
     6841 catcggcatg ggggaataac cagacgcgtg cggcacccaa caccagtact gcaaggatgc
     6901 aaaccgggat cgtactccag aaaagtgtcc atagcgccct gccccagcgg ctaaaaaaac
     6961 cgctctgtgc ttccggtatg tcaatttcga ccggtgcctg cgtttgcagt gtttcacgca
     7021 cccatttttg caccagcgtc gcaatcagca acgccatcac cagcccggcc accagacgaa
     7081 tcgccgcaaa accccagccg aggacaaacc ccataaacac cagcgtcgcc gggtttaaca
     7141 ccggattgcc catccagaat gccagcgcac cgcccatcga tacctgttgg cgacgcattc
     7201 ccgccgcgac cggagccgca cagcaggtac acatcatgcc cggcagcgaa aacagcgttc
     7261 ccagcagcgt gccgcgaaag cgcgattgcc caagcgtacg caacaaccag tcacgcggga
     7321 tcagcacctg aatcaacgaa ccgaggatca cccccagcac cgccgctttc catactgcga
     7381 ggaaatagat catcgcgtaa tccaacgccg cttgccacgg attagcatcc gcctgcgcaa
     7441 ggatagattt accgatactg tgggtttcgg ctgcagtaaa cgctttgccg tagtaaggtt
     7501 gccatttcac ataccagaga ccggcaatga caacgagaaa gaaaagcgcg ggtttccacc
     7561 actgaatggg cgttgccgcc tgagatgaag actgaccagt catagcattc cccaggaata
     7621 agttatgata attgagcgcg tgaatattac gctcactatc aattcttggg aataattatt
     7681 cagctctttt gcgtaattct gaagagctta aaatcgtcac gccttcatgc tcaggccgca
     7741 tcgcctctgc cagcatgacc cgcgccacgt ctctcgcatc aatggatttc cagttacctg
     7801 gtaacaaacg gaacagcggc gcaaaaagcg tttcgttcat ccgctgtttg ctacgatcgc
     7861 ccagtaacat cgacgggcga gcaatggtca atttcggcca gttctgggca attaatgctt
     7921 cttccatctc ccctttgacg cggttataga aaaacggcga gtgggcattg gcacccatag
     7981 cactgaccac cagcatatgc tgcgcgccca gtcgccgccc ggttaatgcg gtatccacta
     8041 ccagcgtgta atcggcatga ataaacgcct ctttgctccc cgcttctcgc cgcgtggtgc
     8101 cgagacaaca aaacacaatg tcgatgggtt cggtgacctg cgccaacgcg tcactcagtt
     8161 gcggatcatg gggattaaac accccgggca tatcgcccaa cggacgtcgc gtcggcgcag
     8221 caatggcgtt aactttcggt tcattaatca acatccgcag caggtgaccg cccaccagcc
     8281 ccgttgcgcc tgtaatcagt acctgactca tcttcgctcc tttacagaat tgtctgcctt
     8341 gcgctccccg gctcatgcac cagtcttaat agaccatgag gtaattatcc ccgattgtgg
     8401 aaaattcgcc tcatccaatg caacaacgcg gaggaagcat gagtaagaaa attgccgttt
     8461 taatcactga tgaatttgaa gattcagaat ttacttcacc cgcagacgag ttccgtaaag
     8521 ccggacacga agtgattact attgaaaaac aagcgggtaa aacggtgaaa ggcaaaaaag
     8581 gagaagccag cgtgaccatc gataaatcca tcgatgaagt gacgccagcg gagtttgatg
     8641 ccctgttgct accgggtggt cattcaccgg attatctgcg tggcgacaac cgttttgtca
     8701 cctttacccg tgattttgtg aatagcggca aacctgtttt cgccatctgt cacggtccgc
     8761 agctactgat cagcgccgat gtgattcggg ggcgcaaact gaccgcagtt aaaccgatca
     8821 ttattgatgt taaaaatgcg ggcgtggaat tttacgatca ggaagtcgtg gttgataaag
     8881 atcagctggt taccagccgg acaccggacg atctgccagc gtttaaccgc gaggcgttac
     8941 gcctgctcgg tgcctgagtc gcgtagccaa atcatttttt taccaaagcc cagcgtgttg
     9001 tcggtgaatt tgatttcatc gagccggatt tcccacaccg gggccgacag cattctggca
     9061 accggaaagc gacgattgta cgccttgcgc gcgaggtcgc tttcctcacc ttccagcctg
     9121 cggatctcac ctttaaactg cacaccgcga attaacgcta ccgttttcgg ctgaccgttt
     9181 accgttccgg caaccgcagc ctgcggccca ctcatctgcg cgtggcgcgt tttttcttcc
     9241 gtcaaaatgt agaaggcgac tttctgcgca tcaaaaagat aaaaggcatt agcgcaccag
     9301 agttcccctt cctgctgcac acaccaggtg acaacatgtt gttttgccag ccaacggctg
     9361 atggcgatga gtgtttccat tgctgttctc ttttatactg tgggcctgac cttaacatgc
     9421 gatgcagacg atgacaccct ggtttcttta cttgatccgt accgccgaca ataagcttta
     9481 taccgggatc accacggatg tcgaacgccg ctatcagcag caccaaagcg gcaaaggggc
     9541 gaaagcactg cgcgggaaag gagaactgac gctggcgttt tctgcgccag tgggcgaccg
     9601 ttcgctggca ctacgggcgg aatatcgcgt taagcaactg accaaacggc aaaaagagcg
     9661 tctggtagcg gaaggcgcag ggtttgcaga actgttgagc agcctgcaaa ccccggagat
     9721 taaaagcgat tgaaatgctc gtgatactca accagaccgg ttacgccatt cagcgcgtca
     9781 tccgctaaac gatgtaccag gaaggcgctt tcagtgcccg gccagcggca acgcagatcg
     9841 tgatgcgccg ccagttcaaa gccgaaacga ctgtacagcg ccggatcgcc cagcgtcacc
     9901 actgcggcat agccgaactc attaagcgaa tcgagtcctt catagaccag ttggcgtgcc
     9961 agcccctgtc cacggtattt ttcatcaacc gccaacggtg ccatgccgac ccattgcagg
    10021 tcttcgccct gcacatcaac cggactaaat gccacatagc caatgacctg accttcgtca
    10081 tctgtcgcca ccagccccag cgtcagaaag ccatcttcac gcagatcgtg aaccagcttc
    10141 gcttccgcat cactttcgaa tgagcgacgc agcagggcat caatacccgg cgcatcaatg
    10201 ggaatttcta ctcgaattag catggttcac ctaccgatgt ctgtttggtt tcaggcgcgg
    10261 ttttcattcc cgcctcaaca aaatccgcca gttgcagcag catcatgcgt aaggctttcg
    10321 gcatttgctc cagttcaatg gcgtccatca ggtttttcac atacaccccc agctccgtat
    10381 cgccttcaat caccagccga cgctggaaga agagcgtatc cggatcttgt ttacgcgccg
    10441 caatcatcag cagatcgctg gcgtcggcac taaaactcac atcagcttgc gtgttctggc
    10501 tgaccaccag tttgccattc accaccgagg taaaccattg caggtcaata tcacgcacat
    10561 gaatacttaa ccagcggcct tcaagaaact ccagctcgcc atcatccagc gcctgacgga
    10621 attgccagct taagacctgc tcaagaacct ggcgttttag cgcaaatggc gtcagtttta
    10681 ccggtacact caacagagat ggccccaaat gcacaatacg ggaacgcagt ttatccaaca
    10741 cgagttttac tccctgtttc aacaatcatc ctattttgcc atatcagaaa aataacatag
    10801 cggtataaat caacaattcc atatgaaatt gctgctacca ccaatacaac tttaactgcc
    10861 ttaaatcaaa aattgtcgca gcaaggttaa ctaaaatccc agttcgttaa catttttgcg
    10921 ttttgatagc gcaaccttca ggaaaaatta tggagctgct ctgccctgcc ggaaatctcc
    10981 cggcgcttaa ggcggccatc gaaaacggcg cagatgctgt ttatatcggg ctaaaagatg
    11041 ataccaatgc ccgtcacttc gccggcctta actttaccga gaaaaaattg caggaagcgg
    11101 tgagttttgt ccatcaacat cgccgcaaac ttcacatcgc gattaacact tttgcgcatc
    11161 cggacggtta cgcccgttgg cagcgtgccg tggatatggc ggcgcagctg ggtgccgacg
    11221 cgctgatcct cgccgacctc gccatgctgg agtacgccgc cgagcgttac ccgcatattg
    11281 aacgtcatgt gtcagtgcag gcttcggcga ccaatgaaga ggcgattaac ttttatcatc
    11341 gccattttga cgttgcccgc gtggtgctgc cgcgcgtgtt gtcgattcat caggtgaaac
    11401 agctggcacg ggttactcct gtcccgctgg aagtgtttgc tttcggcagc ctgtgcatta
    11461 tgtcggaagg ccgttgctat ctgtcgtcgt atctgacggg tgagtcgccc aacactgtgg
    11521 gtgcgtgttc tccggcccgt ttcgtgcgct ggcaacaaac gccgcagggg ctggaatccc
    11581 gcctgaacga agtgctgatc gaccgttatc aggacggcga aaacgcaggt tatccgacgc
    11641 tgtgtaaagg gcgttatctg gtggacggcg agcgctatca cgcgctggaa gaaccaacca
    11701 gtctcaatac cctggaactg ctgccggagt taatggctgc gaatattgct tcggtgaaaa
    11761 ttgaaggccg ccagcgtagc ccggcgtatg tcagccaggt ggcgaaagtc tggcgtcagg
    11821 ctatcgaccg ttgtaaggcc gatccgcaaa acttcgtacc gcaaagcgcg tggatggaga
    11881 cgctcgggtc gatgtccgaa ggcacgcaaa cctcccttgg cgcgtatcac cgtaaatggc
    11941 agtgagaaga gcaatgaaat attccttagg gccagtgctg tggtactggc caaaagagac
    12001 gctggaagat ttttatcagc aagccgccgc cagcagcgcc gacgtgattt atcttggtga
    12061 agcggtatgc agcaagcgcc gggcaaccaa agttggcgac tgtctggaga tggcaaaatc
    12121 gctcgccgga agtggcaagc agattgtgct ctccacgctg gcgctggtgc aggcatcctc
    12181 tgaactgggc gaactgaaac gctatgttga gaacggtgaa tttctgattg aagccagcga
    12241 tctcggcgtg gtgaatatgt gcgctgaacg caaactgccg ttcgtcgccg ggcacgcgct
    12301 gaactgctac aacgcggtga cgctgaaaat attgctcaaa cagggcatga tgcgctggtg
    12361 tatgccggtg gagctttccc gcgactggct ggtgaatctg cttaatcagt gcgatgagct
    12421 gggcattcgc aaccagtttg aagtggaagt cctgagctac ggtcatctac cgctggccta
    12481 ctccgcccgc tgctttaccg cgcgttcgga agaccgcccg aaagatgagt gtgaaacctg
    12541 ctgcattaag tatccgaacg ggcgcaacgt gctgtcgcag gaaaaccaac aagtgtttgt
    12601 actcaatggc attcagacca tgagcggcta cgtttacaac ctcggtaacg agctggcatc
    12661 catgcaggga ttggttgatg tagtccgcct gtcaccgcag ggcactgaca ctttcgcgat
    12721 gctcgacgcc ttccgcgcta atgaaaatgg cgctgtgcca ctgccgttga cggcaaacag
    12781 cgactgtaac ggctactggc ggcggctggc aggactggag ctgcaagcct aagtaaatag
    12841 ctcactttgt taacaacttt aactattctt taatgcagta ttaaagatta atcggtaaca
    12901 aagtgagctg ttatgactga taaaaccatt gcgttttcgc tactcgatct ggcccccata
    12961 cccgaaggct cttcagcgcg ggaagcgttc tcccactctc tcgatctcgc ccgtctggct
    13021 gaaaagcgcg gctatcatcg ctactggctg gcagaacacc acaatatgac cggcattgcc
    13081 agtgctgcaa catcggtatt gattggctat ctggcggcga ataccaccac gctgcatctg
    13141 gggtctggcg gcgtgatgtt gcctaaccac ttaccgttgg tcattgccga acagttcggc
    13201 acactcaata cgctctatcc ggggcgaatc gatttggggc tgggtcgtgc tccgggtagt
    13261 gaccaacgga caatgatggc gctacgtcgt catatgagcg gcgatattga taatttcccc
    13321 cgcgatgtcg cggagctggt ggactggttt gacgcccgcg atcccaatcc gcatgtgcgc
    13381 ccggtaccag gctatggcga gaaaatcccc gtgtggttgt taggctccag cctttacagt
    13441 gcacaactgg cggcgcagct tggtctgcca tttgcgtttg cctcacactt cgcgccggat
    13501 atgttgttcc aggcgctgca tctttatcgc agtaacttca aaccgtcggc tcggctggaa
    13561 aaaccatacg cgatggtgtg catcaatatt atcgccgccg acagcaaccg cgacgctgaa
    13621 tttctgttta cctcaatgca gcaagccttt gtgaagctgc gccgtggcga aaccgggcaa
    13681 ctgccgccgc cgattcaaaa tatggatcag ttctggtcac cgtctgagca gtatgacgtg
    13741 cagcaggcgc tgagtatgtc gttggtaggc gataaagcga aagtgcgtca tggtttgcag
    13801 tcgatcctgc gcgaaaccga cgctgatgag attatggtta acgggcagat tttcgaccac
    13861 caggcgcgcc tgcattcgtt tgagctggcg atggatgtta aggaagagtt gttgggatag
    13921 tgtgtcttaa cgcgggaagc cttatccgag ctggcaacgc tgtcctacgt aggcctgata
    13981 agcgaagcgc atcaggcatt gtgtaggcag cagaaatgtc ggataaggca ccgctgatta
    14041 ctgatacacc ggcagtaaat taaagctcga taaaatatgc accagtgcgt tgccgacgcc
    14101 aaacaccaga atcagcgcaa tcatcggctt gccaccccag acgcggaatt tcgggctgcc
    14161 aaagcgttta cgcgatgcgc gggccagcag tgccggaaca attgccgccc agatggtagc
    14221 cgctaaacca gcataaccaa tggcatacag gaatccgttc gggaacagca gaccacccac
    14281 aacaggcggg gcaaaggtca gcaatgccgt tttcaagcgg cccacagccg agtcgtcgaa
    14341 accaaacaga tctgccagat agtcaaacaa acccagcgtt acgccaagga acgaactcgc
    14401 taccgcaaag tttgagaaca cgaccagcag aaggtccagg ctacggctgt tcagcacgcc
    14461 gcttaatgct tgtaccaaca catcaatatt accgcccttc tcggcgatgc cgataaactc
    14521 cggacgaggg atgttgccca ttgtcgccag caaccagatg gtatacagcg ccagtgccat
    14581 cagcgtaccg tacaccaggc atttcacgat ggttttcgga tctttgccgt aatacttcat
    14641 caggcttggc acgttaccgt gataaccaaa cgacgccagg cagaacggca gggtcatcag
    14701 caaatacggc gcataagatg cattgctttc ggcaacgttg aacaatgtcg caggctgcac
    14761 atgccccagc aggctaccga aggtgaggaa gaaggtaatg actttcgccc ccagcacaat
    14821 cgccgtcatg cggctgacgg ctttagtact caaccacacc acaaacgcta ccagcagtgc
    14881 aaaaccaaaa cccgccgccc gtgccgggac gtttagcgac atctctgcga aggtgtgatg
    14941 cagaatcgaa ccactggcag aaatataggc ataggtcagg atatagagca caaaggcaat
    15001 ggaaatgccg ttgaccacgt tccagccttt gcccagcaaa tctttggtga tggtgtcaaa
    15061 actcgaaccg attcggtaat tcaggttagc ttccagaatc atcaagccgg aatgcagcat
    15121 acagaaccag gtaaagatca gcgctgccat tgaccagaaa aaccacgccc cggacatgac
    15181 cactggcaga gaaaacatcc ctgcgccaat aatggtgccg ccgataatca ccacgccgcc
    15241 aagcagcgac ggtgacgttt gggtggtggt tcgtgttgcc atgagggctt ctctccagtg
    15301 aaaaatagtg cgactgcgtt gttatgcatt gcactgtacc agtacatgag tacaaaagac
    15361 agaaaaaaag ccccgatggt taaaatcggg gctgtatata ttattttaca gattgtgttc
    15421 gctgttcagc gatgattacg catcaccacc gaaacgacga cgaccggtag agtcatcacg
    15481 acgcggtgcg cggccttcac gacgttcgcc gctaaaacga cgaccatcac cacgaccacc
    15541 ttcacggcgt tcaccgctga agttacgacc gccttcacga cgctcgccac cgaaaccacg
    15601 accaccgcca cgacgctcac cgccagtatg cggctgtgca tcgcccagta actgcatgtt
    15661 catcggcttg ttgagaatgc gagtgcgcgt aaagtgttgc agcacttcac ccggcatacc
    15721 tttcggcagt tcgatggtgg agtgagaagc aaacagcttg atgttaccga tgtaacggct
    15781 gctgatgtcg ccttcgttag caatcgcgcc aacgatatga cgaacttcaa caccatcatc
    15841 gcggcccact tcaatgcggt acagctgcat atcgccaaca tcacgacgtt cacgacgcgg
    15901 acgatcttca cggtcgccac gcgggccacg gtcgttacga tcgcgcggac cacggtcatc
    15961 acggtcacgg aattcacgtt tcggacgcat cggcgcatct ggcggaacaa tcagagtacg
    16021 ttcaccctgt gccattttca gcagtgccgc agccagagtt tcgagatcca gctcttcacc
    16081 ttctgcagtc ggctgaattt tgctcagcag tgcgcggtat tgatccagat cgctgctttc
    16141 cagctgctgc tgtactttag cggcgaattt ttccagacgg cgtttgccca gcagttctgc
    16201 gttcggcagt tctacttccg gaatagtcag cttcatagta cgttcaatgt tgcgcagcag
    16261 acgacgctcg cggttctcaa cgaacagcag cgcgcggcca gcacgacccg cacgaccggt
    16321 acgaccgata cggtgaacgt aagactcaga atccatcggg atatcgtagt taaccaccag
    16381 gctgatacgc tcaacgtcca ggccacgggc cgcaacgtcg gtcgcaatca ggatgtccag
    16441 acgaccgtct ttaaggcgtt ccagtgtctg ctcacgcagc gcctggttca tgtcaccgtt
    16501 cagcgcggcg ctattgtagc cgttacgctc aagagcttca gccacttcca gagtcgcgtt
    16561 tttggtacga acgaagataa tcgccgcatc aaaatcttcc gcttccagga aacgtaccag
    16621 tgcttcgttt ttacgcatac cccacacagt ccagtagctc tggctgatgt caggacgggt
    16681 agtcacgctg gactgaatac gcacttcctg cggctctttc ataaagcggc gggtaatgcg
    16741 acgaatcgct tccggcatgg ttgcagagaa cagagcggtc tgatgacctt ccgggatctg
    16801 cgccataatg gtttcaacgt cttcgatgaa gcccatgcgc agcatttcgt cagcttcatc
    16861 cagaaccaga ccgctcagtt tagagaggtc cagagtgcca cgtttcaggt ggtccagcag
    16921 acggcccgga gtaccgacaa cgatctgcgg cccctgacgc agggcgcgta attgcacgtc
    16981 ataacgctgg ccgccgtaca gggcaaccac atttacgccg cgcatgtgtt tagagaaatc
    17041 cgtcattgct tcagcaacct gtaccgccag ttcgcgggtc ggtgccagca ccagaatctg
    17101 tggtgctttc agctcaggat caagattctg cagcagaggt aaagagaatg ctgcagtttt
    17161 tccgctcccc gtctgggcca tacccagaac gtcgcggcca ttcagcagat gtggaataca
    17221 ctctgcctga attggagatg gtttttcgta acccagatcg ttaagggctt caaggatagg
    17281 agccttcagg cccagatctg caaaagtggt ttcgaattca gccatgtagt acgtgtgcct
    17341 caaaattaat ggcggccagt ctacataact catcatgaaa ttgatcagca attttcattg
    17401 aaaagtgtga accggctcaa agtaggtgta ttaacgaaca acaacgccct cacccgttaa
    17461 ggtgatggca atcaaaaaag attacgggct gatgtgtacg tcagctattg ctggtccgat
    17521 tctgccaggt catcttggtc ctggcccagg agcgataatt ccaacaatgc gtatcggtgc
    17581 tcaacaaagt tatgaacgtt gttggcaacc gccagtttga acagtgccgt ggcgctgtcc
    17641 aaatccccca gacttaggta gtacttacct aaatagaagt tggtttcact gagatgctca
    17701 gcgagcgagg tgttatccgt tgcgtccgcc ttgagccttt ccattaacgt ttgttcgcta
    17761 atgttgccca ggtagaactc gacaatgttc catccccact gttccttatc cgatttttcg
    17821 aagtgctgtt tcaatacttc tttagcctgc ttctcatcga gcttctgctc ggcgagataa
    17881 agccacagac tacggaaagg atcattggga tcgtcttgat aaaacgccag cagatcatct
    17941 tgcgctaact tgtcacgacc gccgtaatat aatgcaatcc cgcgattcaa gtgcgcgtag
    18001 ttgtaagttg gatcaagctc aagtacagaa tcaaacgctt cataggcagc atcaaaattg
    18061 cctgcctgcg ttaaatatat gcctaagtaa ttgaatactt caggcatatc cggtcggatt
    18121 gccagcgctt gcgaaaaatc gttacgcgct aatgccctca gaccgagact atcatacaac
    18181 actccgcgct catataaaag ctgtgcgcgt tcgtcatcgg ttaaagcccg actggcaagg
    18241 atttgttcca tacgtgccag aatcacttcc tgctgtaaag tcggttgcaa tggtaccgcg
    18301 aggacttcac ttttacgcca ggaagtatta ctgcatcctg caagcgtaag tgctgtcgca
    18361 acgaaacacc agcgcaaaaa aggcttcatt tcccactccc gaagaccacg gttgaatgaa
    18421 cgtcctgttc ccggttgcta acaaggcgtc ctgcccggtt aaaagccccc cgccgcagcg
    18481 gagggcaaat ggcaacctta ctcgccctgt tcagcagccg gagcttccgg tgctgcagca
    18541 ggttgagact gctcagtcgc ttctttaatg ctcagacgga tacggccctg gcgatcaact
    18601 tccagaactt tcaccggtac ttcctgaccc atctgcaggt aatcggtcac tttctcaacg
    18661 cgtttgtcag cgatttgaga gatgtgtacc agaccttctt taccgccgcc gatggcaaca
    18721 aatgcgccaa agtcaacgat acgggtcact ttaccagtgt agacgcggcc cacttcgatt
    18781 tctgcagtga tctcttcgat acgacgaata gcatgtttcg ctttctcgcc gtcggtcgct
    18841 gcaatcttca cagtaccgtc atcttcgatt tcgatggtgg tgccagtttc ttcggtcagg
    18901 gcacggataa cagagccgcc tttaccgata acgtctttga tcttgtccgg gttgatcttg
    18961 atggtatgga tacgtggtgc gaactcagag atatcgccac gcggcgcgtt gatcgcctgt
    19021 tccattacgc ccaggatatg cagacgcgca cctttagcct ggttcagcgc aacctgcatg
    19081 atctctttgg tgataccttc aattttgata tccatctgca atgcagagat accctcgcgg
    19141 gaacccgcaa ctttgaagtc catatcgccc aggtgatctt cgtcgcccaa aatgtcagac
    19201 agtacaacgt agttgtcgcc ttctttcacc agacccattg cgatacccgc aacggcagct
    19261 ttgatcggca cacctgcgtc catcagcgcc agagacgcgc cgcacacgga agccatagaa
    19321 gaggaaccgt tggattcagt gatttcagac acaacacgta cggtgtacgg gaatttgtcc
    19381 atatccggca tgactgccag cacgccgcgc ttcgccagac gaccgtgacc aatttcacga
    19441 cgcttcggag aaccgaccat gccggtttcg cctacggagt acggagggaa gttgtagtgg
    19501 aacaggaagg tatcggtacg ttcgcccatc agttcatcaa gaacctgcgc gtcacgagca
    19561 gtacccagcg ttgcggtaac cagcgcctgc gtttcaccac gggtgaacag cgcagaaccg
    19621 tgagtacgcg gcagcacgcc agtacgcaca tccagaccac ggatcatatc tttttcacga
    19681 ccgtcgatac gcggttcgcc tgccagtacg cggctacgaa caacgttttt ctcgatcgcg
    19741 tgcagaattt cacccagttc gttttcgtcc agggtttcgt cttcagcaag cagcgtcgcg
    19801 atggtttcag atttgatgac atcaacctgc gcataacgct cttgtttgtc ggtgatgcgg
    19861 taagcatcgc tcaggcgagc ttcagccagt gcagcaacgc gcgcgttcag cgcttcgttt
    19921 accggctccg gctgccagtc ccaacgtggt ttaccggctt ctttcaccag ttcattgatg
    19981 ttctgaataa caacctgctg ttgttcatga ccgaacacta ctgcgcccag catctggtct
    20041 tcgctcagca gttcagcttc agattcaacc atcagtacag cggcttcagt accggcaaca
    20101 accagatcca gtttgctctc tttcaactcg tcctgagtcg ggttcagtac gtactggtca
    20161 ttgatgtaac ctacgcgagc agcaccaatc ggaccattga acggaatacc agacagagac
    20221 agtgctgcgg aagcaccaat catcgcgacg atatccgggt taacttgcgg gttaacagaa
    20281 accacggtgg cgataacctg aacttcgttg acgaagcctt ccgggaacag cggccgaatc
    20341 gggcggtcaa tcagacgcgc gatcagggtt tcgccttcgc ttgggcggcc ttcacgacgg
    20401 aagaagctac ccgggatacg accagcagcg taggtacgct cctgatagtt aacggtcagt
    20461 gggaagaagt cctgacctgg tttggctttt ttctggccaa caacggtaac aaataccgcg
    20521 gtgtcatcca tgctaaccat aacagcggca gtagcctgac gagccatcat gccggtttcc
    20581 agagtcacgg tgtgttggcc gtactggaat ttacgaacga tcggattaag caatgtaata
    20641 tcctttctct ttcttagaca gtaccttacg gcactggtgt taatacccga tcttctgcgc
    20701 atcctcgcga ctaatgacaa ccctaaccca gctctatgtg ggtaaagcct ctcattagcc
    20761 gcgcgaacct ctgcaacgga agatcattca tagcaacaat acattagttt ccagtgaatt
    20821 gctgccgtca gcttgaaaaa aggggccact caggccccct tttctgaaac tcgcaagaat
    20881 tagcgacgca gacccaggcg ctcgatgagc tgggtgtaac gtgctacgtc tttacgtttc
    20941 aggtagtcga gcagtttacg acgctgagaa accatgcgca gcagaccacg acggctgtgg
    21001 tgatcttttt tgtgctctgc aaagtggccc tgcaggtggt tgatctgtgc agtcagcagt
    21061 gctacctgaa cttcagtaga accggtgtcg tttgcgtcac gaccaaactc agaaacgatt
    21121 ttagctgttg cttcagtact tagagacatt ttaaaactcc aaagtatata gaatgaaagg
    21181 acgccgatct ctaattcagc gatcccagtg tacgttacgc aaagtgttaa acaatttacg
    21241 cgacgttaag cggcagtatt ctactcgtag cgacctgtta tcgcaagacg gttaacatta
    21301 cgccgggtat tcaaccacca ggcgacgagg cgcaacgcgg ccttcatcgt caatttcgcc
    21361 cataccgata aatttgccgt tctcaccttc tgtaacgcga accagtcctt ccagcggcgc
    21421 accggatgta cgaactgggt taccattttt gaagtaaaca gaagacgtta acggaagatt
    21481 caccaccggg tagtccgaag ctggactgtc cattggcatc agtaatggat caagtaactc
    21541 cgcagccgga atatcctgct gttcggcttg ttcgaccagt tcacgcagat gttccagggt
    21601 cacaatccgt tcaaccggat atttacttac cgccagacga cgcaggtaaa taacatgcgc
    21661 gccacaaccg agtttttcac ccaggtcatc aatgatggtg cggatataag tgccttttga
    21721 gcagtgaatt tccagctcca gctcattgcc ttcgtggcga ataaacagca attcataaac
    21781 ggtaatcggg cgcgcttcac gcggtacttc aatgccctga cgcgcatatt cgtacagttt
    21841 tttaccctga tatttcagtg ctgaatacat cgaagggatc tgttcgatat cgccacggaa
    21901 agtatccagc gccgccgcca gttgctctgc gctaaaggtt accggacgtt cttcaacgat
    21961 ttgtccgtcg gcatcagaag tatcggtacg ctgtccaaga cgcgcaatga cccgatagcg
    22021 tttgtcggag tccagcagat actgggaaaa cttcgtcgct tccccgaggc aaattggcaa
    22081 catgccagtt gccagcgggt ccagcgcacc ggtatgcccg gcacggttgg cgttataaat
    22141 ccgtttcact ttttgcagcg catcattgct ggacataccc tgcggtttat ccagcaacaa
    22201 aacgccgtta atgtcgcgac cgcgacgacg aggacgactc attagtcctc cttgctgtcg
    22261 tccgggttaa cacgacgttc ttcgtcatgt ttgaccacgc tggtcaccag gtttgacatg
    22321 cgcatccctt caaccagaga gttgtcgtag aagaaggtca gttccggcac gatacgcagg
    22381 cgcatcgctt tccccagcag gctgcggatg aaaccagaag cttcttgcaa cgctttgatg
    22441 cccgctttaa ccgcgtcttc atctttgtcg ttgaggaacg tcacatatac tttggcatac
    22501 gccaggtcgc gagacatttc gacaccggaa acggtggtca tcatgcccag gcgaggatct
    22561 ttaatttcac gctgcaggat gagagcgatc tctttttgca tttcctgcgc tacgcgctgc
    22621 gggcgaccaa attctttcgc cataataaat tctcctgaca aaaaaggggc tgttagcccc
    22681 tttttaaaat taatttcagg tggaagggct gttcacgttg gcctgataag acgcgccagc
    22741 gtcacatcag gcaatccatg ccggatgcgg cgtaaacgcc ttatcccgca tggaacccta
    22801 aaaaccttaa gcgatggtac gttggatctc gatgatttcg aatacttcga tcacatcgcc
    22861 agtgcggacg tcgttgtagt tcttaacgcc gataccacat tccataccgt tacggacttc
    22921 gttaacgtca tctttgaagc ggcgcaggga ctccagctcg ccttcgtaga taaccacgtt
    22981 gtcacgcaga acgcggatcg ggttgtgacg tttaaccaca ccttcggtaa ccatacagcc
    23041 tgcgatggca ccaaatttcg gcgatttgaa cacgtcacga acttccgcca gaccgataat
    23101 ctgctgtttc agttccggag acagcatacc gctcatcgcc gctttcactt cgtcaatcag
    23161 gttatagatg acggagtagt aacgcagatc caggctttcc gcttcaatca ctttacgtgc
    23221 agaggcatca gcacgtacgt tgaagccaac caggatggcg ttggacgccg cagccagggt
    23281 ggcgtcggtt tcggtgatac cacctacgcc agaaccgatg atcttcactt taacttcgtc
    23341 agtagacagt ttcagcaagg agtcggagat cgcttccaca gaaccctgta cgtctgcctt
    23401 caggacgatg ttcacttcgt gaacttcgcc ttcggtcatg ttggcgaaca tgttctcgag
    23461 tttagatttc tgctgacgcg ccagtttaac ttcgcggaat ttaccctgac gatacagtgc
    23521 aacttcacgc gctttcttct cgtcacgtac aacggtaact tcatcacccg cagccggtac
    23581 gccggacagg ccgaggattt ccaccggaat ggacggaccc gcttccagca cttcctgacc
    23641 cagttcgtta cgcatcgcac gaacacgacc gtattcgaag ccacacagaa cgatatcgcc
    23701 cttgtgcaga gtaccttcac gtaccagaac ggtagcaacc ggaccacgac ctttatcgag
    23761 gaaggattcg ataaccgcac cgctcgccat acctttacgt accgctttca gctccagaac
    23821 ttccgcctgc agcaggatag cgtccagcag ttcatcgata ccagtacccg ctttcgcaga
    23881 cacatgtacg aactggcttt caccgcccca ctcttccggc aggatgccgt actgggagag
    23941 ttcgttctta acgcgatccg gatcggcttc tggtttatcg atcttgttca ctgcaacgac
    24001 caccggaacc tgcgccgctt tcgcgtgctg gattgcttcg atcgtctgcg gcattacgcc
    24061 gtcgtcggca gcaacaacca ggactacgat gtccgttgcc tgcgcaccac gagcacgcat
    24121 tgaagtaaac gcggcgtgcc ccggggtgtc caggaaggtg atcatgccgt tttcagtttc
    24181 aacgtggtat gcaccaatgt gctgggtaat gccgcccgct tcgccagagg ccactttcgt
    24241 tgaacgaatg tagtccagca gagaggtttt accgtggtca acgtgaccca tgatggtcac
    24301 aaccggcgcg cgcggttcag ccgcagcacc cgtgtcacgg tcgctcatta ccgcctcttc
    24361 cagctcgttt tcacgacgca ggataacttt atggcccatc tcttcagcaa ccagctgtgc
    24421 ggtttcctga tcgataacct ggttgatggt tgccattgcg cccagtttca tcatcgcttt
    24481 gatgacctga gagcctttaa ccgccatctt gttcgccagt tcgccaacgg tgatagtttc
    24541 gccgatcaca acgtcacggt taacggcctg agcaggcttc tggaagcctt gctgcagcga
    24601 agaaccttta cgttttccgc ctttaccgcc acgtactgct gcgcgtgctt cttcacgatc
    24661 agcttttgat tcagcgtgtt tgttgccttt cttcggacgc gctgctttcg cgttacgacc
    24721 acggccacgg ccgccttcga cttcacgatc gctttcgtct tctgcctggc gagcatgttg
    24781 agaagtagtg acgtgataat cgctggaatc ttcagtcggt tccgcgttat cagtccattt
    24841 gttttcttcc gccatacgac gtgcttcttc agcaacgcga cgtgcttctt cttcgagttt
    24901 acgacgcgct tcttcttcag ctttacgctt gagctctgca gcttcctgct cacggcgggc
    24961 tttttcagcc tgggcgtttt tagtcatatc gtcttgttga ttgctcactt tgtctttttc
    25021 cgcagcttca cgtttcgctt gttcagcagc ttcacgctta gcttgttctg cggcctcacg
    25081 ttcagctttt tgttgcgcct cgcgtttagc cgattcttct gcctcacgac gggcttgctc
    25141 ttccgctgca aggcgttcag cctcttgcgg atcgcgtttc acaaaggtgc gtttcttgcg
    25201 gacttcgatt tgtaccgatt tgctttttcc accggtacca ggaatgttaa gggtgctgcg
    25261 tgttttacgt tgcagcgtca atttgtccgg gcctgaattt ttctgattca ggtggtcaat
    25321 caaagtctgt ttctcttgtg cagacacaga gtcgtcagca gacttccgga tacctgcatc
    25381 agcaaattgc tgtaccaggc gttccacgga ggtctgtcgc tctgcggcca gcgttttaat
    25441 cgttacatct gtcatgctgt tccttcctgc tacagtttat tacgcttcgt caccgaacca
    25501 gcaaatatta cgggcagcca taatcagtgc tccggctttt tcgtcggtca acccttcgat
    25561 atcagccaga tcatcaatgc cctgttcggc gagatcttcc agcgtacaaa cgccacgggc
    25621 ggccagtttg aatgccaaat cacgatctac cccttcaagg ttcagcagat cgtcagccgg
    25681 tttgttatca ccgaggcttt cttcctgggc ctgtgcaatg gtggccagtg catttttagc
    25741 acgctcgcgc agtgcttcaa cggtcggctc atcaaggcct tcgatttcca acagctcttt
    25801 catcggcaca taggccagtt cttccagcgt cgagaaacct tcttctacca gaacagtcgc
    25861 gaagtcttcg tcgatgtcga gatatttggt gaaggtgtcg atcgctgcgt gcgcttccgc
    25921 ctgatgctta gcctgcaggt cgtcaacggt cattacgttg agttcccaac cgctcagctg
    25981 cgaagccaga cgcacgttct gaccgttacg gccaatcgcc tgcgccaggt taccagcttc
    26041 aacggcgata tccatggtgt gtttatcttc atccaccacg atagaagcaa cgtctgccgg
    26101 tgccattgcg ttaatcacaa actgcgccgg gttatcatcc cacaggacga tatcgatacg
    26161 ctcgccgccc agttcagtag aaaccgcctg aacacgcgcg ccacgcatac ctacgcaagc
    26221 acctaccgga tcgatacgtt tatcgttggt tttcaccgcg attttcgcac gagaacccgg
    26281 gtcgcgagcc gctgctttaa tttcaatcac ttcttcgccg atttccggca cttcaatacg
    26341 gaacagttcg atcagcattt ccggcttgga acgagtgacg aacagttgcg cgccacgcgc
    26401 ttccgggcga acggaataaa gcacgccacg aacgcggtcg ccagggcgga agttttcacg
    26461 cggcagcata tcttcgcgca ggatcacggc ttcagcgttg ttgcccagat ccagagagat
    26521 gttgtcgcgg tttacttttt tcaccacgcc ggtgatgatt tcaccttcgt gttcacggaa
    26581 ctgatcaacc accatcgcac gttcggcttc acgcactttc tgcacgataa cctgttttgc
    26641 cgtctgggtg gtgatacggt caaaggtaac agactcaatc tgatcttcaa cgtaatcgcc
    26701 caagttcagg ctttcatctt cataacgtgc ggcttcaagg gtgatttcct tggtcggctg
    26761 ggtgacttca tcaacaacta accagcgacg gaaggtgtca aaatcaccgc ttttgcgatc
    26821 gatctgtaca cggacgtcga tctcttgctc atattttttc tttgttgctg tcgccagcgc
    26881 gctttccaat gcttcgaaaa tcttctcgcg aggtagcgcc ttttcattgg atacggcttc
    26941 aactacagcc aaaatttctt tgttcatcgc gggcttttca cctcatccag actattaaaa
    27001 gtggggaacc aggttcgcct tctggatatt actcagcgcg aacacttcat ctttaccttc
    27061 gacggtaact gtgatcattt caccgtctac cgctttgata acgccctgcc atttacgacg
    27121 gttttgtacc gccatacgga gaaccagagt cacctcttct ccgacaaaac gggcgtagtg
    27181 ttcagccgtg aacagtgggc gatcgagacc cggtgaggag acttccaggt tataagcaac
    27241 ggtgatggga tcttcaacat ccagcacagc acttacctgg tggctcacat cagcacaatc
    27301 atcaacattg atgccatctt cactatcaat atagatgcgc agtgtggatg tgcgaccgcg
    27361 aataaattcg atgcctacca gttcaaaacc cagggcctca actggcgcag taatcatctc
    27421 tgttaatttt tgctctaatg tggacaagcc cacccccaag acataaaaaa agggcctaaa
    27481 gcccagttat tctgtagtca gataacaaaa aaccccgata aatcggggct ttatataact
    27541 gaaccctata accgcaactg cggtctggag cactttccag aaggattttt tcaaatccca
    27601 ctacgaaggc cgaagtcttc acagtatatt tgaaaaagga ctctaaggga aagtggttgc
    27661 gggggccgga tttgaaccga cgaccttcgg gttatgagcc cgacgagcta ccaagctgct
    27721 ccaccccgcg cctgaaacgt ggcaaattct actcgttttg ggtaaaaaat gcaaatactg
    27781 ctgggatttg gtgtaccgag acgggacgta aaatctgcag gcattatagt gatccatgcc
    27841 acattttgtc aacgtttatt gctaatcacg tgaatgaata tccagttcac tttcatttgt
    27901 tgaatacttt tgccttcccc tgctctccct taagcgcatt attttacaaa aaacacacta
    27961 aactcttcct gtctccgata aaagatgatt aaatgaaaac tcatttattt tgcataaaaa
    28021 ttcagttaga gcggaaatcc aggctcatca tcagttaatt aagcagggtg ttattttatg
    28081 acgacgattc tcaagcatct cccggtaggt caacgtattg gtatcgcttt ttccggcggt
    28141 ctggacacca gtgccgcact gctgtggatg cgacaaaagg gagcggttcc ttatgcatat
    28201 actgcaaacc tgggccagcc agacgaagag gattatgatg cgatccctcg tcgtgccatg
    28261 gaatacggcg cggagaacgc acgtctgatc gactgccgca aacaactggt ggccgaaggt
    28321 attgccgcta ttcagtgtgg cgcatttcat aacaccaccg gcggcctgac ctatttcaac
    28381 acgacgccgc tgggccgcgc cgtgactggt accatgctgg ttgctgcgat gaaagaagat
    28441 ggcgtgaata tctggggtga cggtagcacc tataaaggaa acgatatcga acgtttctat
    28501 cgttatggtc tgctgaccaa tgctgaactg cagatttaca aaccgtggct tgatactgac
    28561 tttattgatg aactgggcgg ccgtcatgag atgtctgaat ttatgattgc ctgcggtttc
    28621 gactacaaaa tgtctgtcga aaaagcctac tccacagact ccaacatgct tggtgcaacg
    28681 catgaagcga aggatctgga atacctcaac tccagcgtca aaatcgtcaa cccgattatg
    28741 ggcgtgaaat tctgggatga gagcgtgaag atcccggcag aagaagtcac agtacgcttt
    28801 gagcaaggtc atccggtggc gctgaacggt aaaaccttta gcgacgacgt agaaatgatg
    28861 ctggaagcta accgcatcgg cggtcgtcac ggcctgggca tgagcgacca gattgagaac
    28921 cgtatcatcg aagcgaaaag ccgtggtatt tacgaagctc cggggatggc actgctgcac
    28981 attgcgtatg aacgtctgtt gaccggtatt cacaacgaag acaccattga gcagtatcac
    29041 gcgcatggtc gtcagttggg ccgtctgctg taccaggggc gttggtttga ctcccaggcg
    29101 ctgatgctgc gtgactctct gcaacgctgg gttgccagcc agatcactgg tgaagttacc
    29161 ctggagctgc gccgtgggaa cgattattca atcctgaata ccgtctcaga gaacctgacc
    29221 tacaagccag agcgtctgac gatggaaaaa ggcgactcgg tgttctcgcc agatgatcgt
    29281 attggtcaat tgaccatgcg taatctggat atcactgata cccgagagaa actttttggt
    29341 tatgccaaaa ctggcctgct ttcctcctct gccacttcag gcctgccgca ggtggagaat
    29401 atggaaaaca aaggccagta attcgacatc aaccctgccc ttcaggggtg gggttacctt
    29461 gcttatcctc tgtaaactcc tttctgagca tcacataatc gaaaacatca tggtttgcat
    29521 ccacaacccg tgagtctcct ttatagtgtg caatcacttc ctcaagcgtt tgcggctgtt
    29581 ccggctttgt tacccccatc cacgcattaa taaggtaatt attgtaagct gtcgaaaaga
    29641 tattgttttc cgttgtgcga tccacgccat ccccaagaac ggggctatac cagataaaca
    29701 tcgggacatg atatgcctgc tggctagcct ccctgcctcc atgaaaatag acgttcttct
    29761 tcgtagggtc acgttccagg ccgtggtcgg caaaatacat gaccgaggcg cggcgatctt
    29821 ttaataattc aaaaacctga cctagcaaac tatctgtgta atgaatggag ttgtcatagc
    29881 aggcatcctg atcgtcctgc ggttgaaaca cggcgctgga ttgcggatag gcgctacaag
    29941 ccggttcatg gcttccattt aaatgaagaa caatcagttt cttttgctgc gtattttgct
    30001 gtaatgcttg cgataaatgc ggcaacaaca attcatcaaa tcctctgaca taaactgttt
    30061 ccatggcgcg catggcgata ctggtaactg ctgtaccatt ctgccgaaaa gcggattgcg
    30121 agcttagcca gaaagtctga aatcctgctt gattagccat attaataatg ttgtcggggt
    30181 aattatgaat gtcatgactc aaaacagaat cagcagttaa agaaagggga accgacagcg
    30241 cagtgtaagg tgcgccgctt attgcttgat taaacagttt gatctgtttt ctttgtgctt
    30301 caacttgcgg tgtcgtagag cgtgtatatc catacaaaga catattgtcg acacgtacag
    30361 actcccccac aatcaacacg taggtatcaa tacttgtatc cctgactgat aattgaaaat
    30421 acggcaccgt gtttgcaatt gagagtaatc tttgatgctc tttcgctgct aaagcaaaat
    30481 agttgagatt gaaaaacggc gtataggtag caaatcacga cgctagtata tatggactga
    30541 acgctttttt attttttgca tctttataag caaattgaca agcggaaaat aaactgcccg
    30601 aaatgacaat cagcaataat attcctgtca ctttttttgt cggcaaggaa acatcatatt
    30661 ttattattac tgccaaaaaa agaagggata aaaaagcaaa agcacataga taagggatat
    30721 acatccccag cattttcaca acttcatccg gatcactctg cagcacacta atcgcaaatc
    30781 catcattgaa agttgtacca aaggtacacc atgaatagag gctaatactc atgtctgccg
    30841 cagtcagtaa cacaaatggt gcggcaacaa gcaggcgaag cggaagggat gatatacgtt
    30901 taacaaccag gatggtcagg acgaagaaca gcaatcgctg aaccatatgc gctccggaag
    30961 aggcgactaa gttcgtaata ccaaaaacaa taacacaaag atacaacaac caatgagatt
    31021 taaaatcttc tagcaaattt gttgaatact tcatccagaa atccaagttc acaaccaata
    31081 taaacaatta tcaacaatgt tcaattttat atcagacatg tttttcaact ctgatccata
    31141 tcagaccgaa acactgttag ttttttcttt ttaaatgatg cgaccattgc gttctcagta
    31201 attaaataac attatcaatg cgtctctaaa cattacgcag aacgtagcca tcagtagtta
    31261 tactgacagg agcaggatgg tttatcgaga agtaaacata tccgtattcg taccggaact
    31321 ggagttgaca caataaagtg ccaattatgt cagcagtagg gaaaagttta taactaaggc
    31381 gtaccggcac catcgtttca aggtaccagc tacgagtaaa gcaactggac gagatacaga
    31441 tacctgacaa ccattcatca gaccaggacc aaaacgaaaa gagacgcttt tcagcgtctc
    31501 ttttctggaa tttggtaccg aggacgggac ttgaacccgt aagccctatt gggcactacc
    31561 acctcaaggt agcgtgtcta ccaattccac cacctcggca cggatactac tttttagttc
    31621 gggatatcgc tggtcggctt agccggagca gctggctgag tttgttcggt tttcgccggt
    31681 gcactcagat tttcccattc gctacctcta ttggttttgt tgctattgat gttacccagc
    31741 accagactga tgatgaagaa taacgttgcc agcagcgccg tcatgcgggt catgaagtta
    31801 ccagaaccac ttgaaccaaa cagcgtagcg gaagcgcctg ctccgaagga ggctcccata
    31861 tcagcgcctt taccttgctg cagcatgatc agaccaacaa ggccaattgc cacaataagg
    31921 aaaactacta aaagagcttc gtacataatc aacctgttcc ttgcggagtt gccgcgtacc
    31981 aatgcttcaa ccaataaagc gggagttttt aatctttccc actgaagcgg gtgtgaatac
    32041 taaccaaaac gaatgacctt cgcaagggca attttatcgc attgtatcaa ctgcggaaaa
    32101 aaacagcaaa accgatgtgt tcgccgagaa aaaaggcgac aatcgccgcc tttttagcca
    32161 gttatctaac gctttaaacg gcttttactg catcggcgat gcggtgtgca aattcagtca
    32221 cctgcgcttc gtcttcgcct tccaccatca cgcgaattaa cggttcggtg ccggatttac
    32281 gcagcaacac gcgtccacgg ttgcccagcg cagcttcaac ctctgcggtc acggctttaa
    32341 ctgactcatg ctcaagcgga tcgccgctac ctgcggtgta acgtacgtta accagaatct
    32401 gcgggaacat tttcatgccg ctgcaaaggt cgtgcaggct catatggtta cgtgccatcg
    32461 ccgccagcac ctgcaagcca gcaacgatgc cgtcaccggt agtggtttta tccagcagga
    32521 tcacatgacc ggaattctct gcaccgatac gccagccttt ctcctgcatt ttttccagta
    32581 cgtagcggtc acccactttc gcgcgcgcaa atggaattcc taactgtttc agcgccagtt
    32641 caagccccat gttgctcatc aatgtaccca cagcaccacc acgcagctgg ccctgacgaa
    32701 gaccttcacg cgcgatgata tacatgatct gatcgccatc gactttattg ccttcatggt
    32761 caaccataat cacgcgatcg ccgtcgccgt cgaaggcaat accgagatcc gctttttcag
    32821 ccagcacacg agcctggagc gcgcgaacgt cggtagcccc cacttcggca ttgatgttta
    32881 caccgtttgg ctcacaaccg atagcgataa cgttcgcccc cagttcgcgc agcacgttcg
    32941 gcgcgatgtg ataagtcgca ccgtttgcac aatccaccac aatcttcagt tcactgaggc
    33001 taagttcgtt cgggaacgtg gctttgcaaa actcgatata gcgacccgcg gcatcaacga
    33061 tacggctggc tttacccagt tctgccgaat caacgcagct gatctccttt tccatttccg
    33121 cttcgatggc ctcttctacc gcatccggca gtttagtgcc gtcgatagag aagaatttaa
    33181 tcccattatc gtagaacggg ttatgcgatg cagatatcac aattccggcc tctgcgcgga
    33241 aggtacgcgt cagataagcc acggccggtg ttggcatcgg gccagtgaag agtgcggaaa
    33301 ggcccgctgc cgccagaccc gcttccagtg ctgactccag catatagcca gaaatacgcg
    33361 tgtctttacc aataataatc ttacgggagc cgtggcgcgc cagcacttta cccgcggccc
    33421 aacccagctt aagcacaaaa tcaggtgtga tcggcgcatc ccctacacga ccacgaatcc
    33481 catcggtacc gaaatattta cgattactca tagcgtttgt tttcctttgc agacagagtg
    33541 gcttccacca cccgcatcgc ttctacggtt tctttgacgt catgaacacg aatgatgtgc
    33601 gcgccttgca ttgcggcaat gaccgcacag gccagactac cgctcaggcg ctcggacggc
    33661 cccacgttca gcagctgccc aatcatcgat tttcgtgaca tacccaccaa cagcggcagg
    33721 ttgaaatggt gaaattcagc caggcgcgcc agtaatgaat agttatggga gagattttta
    33781 ccgaaaccga atccggggtc gagcaacaat ttctcttttg cgatacccgc ctgctcgcaa
    33841 cgtgctattt gctcaataaa gtagcgattc acttctgcaa agacatcgtc atacttcgga
    33901 gcttcctgca tggtttttgg atttccctgc atatgcatca gacaaaccgg taaaccggtt
    33961 tctgcagccg cctccagagc gccaggttcg gaaagggagc ggatatcatt aataatgtga
    34021 gcgccaactt tcgctgactc acggatgact tctggtttgg atgtatcgac cgagatccag
    34081 acttcgaagc gttgagcaat tgcctcaacc acaggaataa cacgttgcaa ctcttcttca
    34141 acgctaactt ccgccgcccc tgggcgcgtg gactcgccac caacgtcaat gatcgtcgcg
    34201 ccagcattga tcatcagatt cgcatgtttc accgcatcta tcagcgagtt atgcgtgcca
    34261 ccatccgaaa aggaatcagg cgtgacgttg aggatcccca ttacgtgagg atggctaagg
    34321 tccagtgaag taccctgggc aaagagtttc attatgttat ccctggtatg aattgataag
    34381 aaaaaacccc ggagcacgcc ccggggtttt cggtacaaat acagtcatct gatgcgggaa
    34441 cttacttgtc gcctaactgc tctgacatgg tgttacccgg gttcggcgta cgcggttcat
    34501 caaccggacg aggagccttt ggactaccat tgtcgccaga attgttagaa gcgcctggtt
    34561 cttcccagcc cgctggcgga cgtacatcgc gacgtgccat caggtcatca atctgcggtg
    34621 cgtcgatagt ctcatatttc atgagagcat ctttcatcgc atgcagaata tccatattgt
    34681 cggtcagaag ctgacgcgca cgattatagt tacgctcaat cagtgctttc acttcctggt
    34741 cgatgatacg tgcagtttca tcggacatat gtttcgcttt cgctacgcta cggccgagga
    34801 atacttcacc ttcttcttcc gcgtacagca gtggacccaa tttctcagag aagccccact
    34861 gagtcaccat gttacgtgcc aggttggtcg caactttaat atcgttggac gcaccggtag
    34921 aaacatgttc cggcccgtag atgatctctt ctgccagacg accaccgtac agcgtagaaa
    34981 tctggctttc cagtttctga cggctggcgc tgattgcgtc gccctcaggc aagaagaaag
    35041 tcacacccag cgcacgaccg cgtgggataa tcgtcacttt atgcaccgga tcgtgttccg
    35101 gcaccaggcg accgataatc gcatgacccg cttcgtggta agccgttgat tctttctgcg
    35161 cttccgtcat caccatggag cgacgttccg cacccatcat gattttgtct ttcgctttct
    35221 cgaactcaac catcgacaca acgcgtttgt tgccacgagc agcgaacagt gccgcttcgt
    35281 tcaccaggtt cgccaggtca gcaccggaga aaccaggagt accacgggca atgattgccg
    35341 cgtcgatatc gggtgccaat ggtacgcgac gcatgtgaac tttcaggatc tgctcacgac
    35401 cgcgaacatc tggcaagccg accacaacct ggcggtcgaa acggccagga cgcagcaggg
    35461 ccgggtcgag aacgtccgga cggttagtcg cggcgataac gatgatacct tcgttacctt
    35521 cgaagccatc catctcaacc agcatctggt tcagagtctg ttcacgttca tcgtgaccac
    35581 cgcccagacc agcgccacgc tggcggccta cggcgtcgat ttcatcgata aagatgatgc
    35641 acggtgccgc tttcttcgcc tgttcgaaca tgtcacgaac acgggatgca cccacaccga
    35701 cgaacatttc tacgaagtca gaaccggaga tagtaaagaa cggaactttc gcttcgcctg
    35761 caatcgcttt cgccagcaac gttttaccgg tacccggagg accgaccatc aggacgcctt
    35821 tcgggatctt accgccgagt ttctggaagc ggctcggctc gcgcagatac tctaccagtt
    35881 cagcaacttc ttcttttgct tcgtcgcagc ccgcaacgtc agcaaaggtc gttttgatct
    35941 gatcttccgt cagcatgcgc gctttgctct taccaaacga catggcacct ttgccaccgc
    36001 cgccctgcat ttgacgcatg aagaagatcc agacaccaat cagcaacagc atcgggaacc
    36061 aggagatgaa gatagaagcc agcaggcttg gttcttcagg cggttcaccg acaaccttga
    36121 cgttcttggt caacaggtta tccagtaatt tcggatcctg aaccggaatg taagtggtat
    36181 aacggttact atctttcttg gtaacgttga tttcacgtcc gttgatacgc gcttcacgaa
    36241 cctggtcgtt attgacctct tgtaggaagg tagagtaatc caccttacgg ccattagact
    36301 cgctgggccc aaagctctgg aatactgaca tcagcacaac ggcaatgacc agccagagta
    36361 ttaggttttt cgccatgtca ctcaagggat taacctctta ttacaactgt gttaaaaaca
    36421 gcgtcaggat actctatatc cagcatcttt caaactttcg tctgaaatct cccggttagg
    36481 gtttacgccc ggtcgctaca atatacactt ctcgcgaacg tgcacgagaa gagtccggct
    36541 tacgaacttt gaccttcgta aacagggagc gaatttccct tagatactca tcgaaacctt
    36601 cgccctggaa caccttcact acaaaactgc cacctggcgc taatacatca cgacacattt
    36661 ctagcgccag ttccaccaga tacatggctc gggggatatc caccgccggt gttccgctca
    36721 tgtttggtgc catatcggac atgacaacct ggactttgct gtcgccaacg cgctccagca
    36781 gtgctttcat caccagttca tcacgaaaat cgccctgaag aaagtccaca ccaacgatag
    36841 gatccatagg taaaagatcg caagcgatga tgcggccttt gccgccaatt tgggtgacca
    36901 catattgtga ccaaccaccc ggagcagcac caaggtcgac aaccgtcatt cccggtttaa
    36961 agagtttgtc actttgctgt atttcatcaa gtttaaacca ggcacgggaa cgtaatccct
    37021 ttttctgtgc ctgttgaaca tatttatcgc taaagtgttc ctgaagccag cggctggagc
    37081 tggcagaacg cttcttacct gtcatttaac tttcccatgg ggataactca tcgtaaccaa
    37141 ttgcgtaaat ttttacgcgc ctatttggtg atatatggga gatggcggta gaatgacccg
    37201 ttttcaatcc caacgtaagc aaaaaatacg atgaatctga gtactaaaca aaaacagcac
    37261 ctgaaaggtc tggcacatcc gctcaagcca gttgttctgc ttggcagtaa tggtttgacc
    37321 gaaggggtgc tggccgagat tgaacaagcg ttagagcacc atgaactcat caaggtgaaa
    37381 atcgccaccg aagatcgcga aacaaaaacc ttgatcgtgg aagctatcgt gcgcgaaacc
    37441 ggcgcctgta atgtacaggt catcggtaaa acgctggtgc tttatcgccc aactaaagaa
    37501 cgtaaaatct cgctgccacg ctaagattat cctaaagtta cacacatttg ctgtgtaaaa
    37561 cgaggggttt tccgcaggca ggagagcaaa atgccacgct ctgttcgttg ataaaaggcc
    37621 gcagagcggc ctttttcctt tctttacaat acatcaacat cttgagtatt gggtaattct
    37681 tacaggtatt ccaccttaat tacttcaaat tctacttcgc cgcccggcgt tttgatgacc
    37741 acaacatcat cttcttcttt gccgatcagg ccacgagcaa taggcgagtt tacagaaatc
    37801 aggttttgtt taaagtcagc ttcgtcatcg ccaacgatgc gataagtctg ttcttcgtca
    37861 gaatccagat tcagcaccgt tacggtagca ccaaaaataa cgcgcccatt gttgggcatt
    37921 ttggtgacat caatcacctg cgcgtttgac agcttggctt cgatgtcttt gatacggcct
    37981 tcgcagaagc cctgctgttc acgagctgcg tggtattcgg cgttttcttt caggtcgcca
    38041 tgctcacgcg cttccgcgat agcagcaatg atttcaggac ggcgcacaga tttcagaaaa
    38101 tccagctctt cgcgtaattt ttcagcgccg cgtaaggtca tcggaatagc ttgcatttgt
    38161 tatacctctt gaatattcct gatggggcaa gtcttcaccc accccggctg ttcggcctgc
    38221 ccggcataac tcctcaccgg gaccagaagc aaaaaaatac cgacccgggt acaagtccca
    38281 ggtcagctac aattcacatt ttgatggtca ttttaccctg aagttcccga agggtcatcg
    38341 tttactttat agggcgttgc gccgtagtat gacggctcga ttccaggttg ttagcgcgag
    38401 attatgcgat tttccagatt tatcatcgga ttgaccagct gtatagcgtt cagtgttcag
    38461 gccgcgaatg ttgatgagta cgttactcaa ctccccgcag gtgccaacct tgccctgatg
    38521 gtgcaaaaag tcggtgcgtc ggcccccgct attgattacc acagtcagca gatggcgctg
    38581 cccgccagta cccagaaagt gattactgcg ctggcggcgt tgattcaact cggccccgat
    38641 tttcgtttca ccacgacgct tgaaaccaaa ggcaatgtgg aaaacggcgt acttaagggt
    38701 gacttagtgg cgcgatttgg tgccgatccg acgttaaaac gtcaggatat tcgcaatatg
    38761 gtcgcgactt tgaaaaaatc tggcgtcaac caaatcgatg gcaatgtgtt gatagatacc
    38821 tccattttcg ccagccacga taaagccccc ggctggccat ggaatgacat gacacaatgc
    38881 tttagcgctc cgcctgctgc cgccatagtt gaccgcaact gtttctccgt ctcgctctac
    38941 agtgccccaa agcctggtga tatggctttt atacgcgtgg catcttatta ccccgttacg
    39001 atgttcagcc aggtacgcac cctcccccgt ggttctgccg aagcgcaata ctgcgaactg
    39061 gatgtggtgc caagcgacct gaaccgcttt acgctgacgg gatgcctgcc acaacgttct
    39121 gagccgctcc cgttggcttt tgccgtgcag gatggagcca gctatgccgg tgcaattctg
    39181 aaagatgagt taaaacaggc gggtatcacc tggagcggaa cactgctgcg ccagactcag
    39241 gttaacgaac ctggaacggt agttgccagt aaacagtcgg ccccgctgca cgatctgctt
    39301 aagattatgc tgaaaaagtc ggacaacatg atcgccgata cggttttccg catgatcggc
    39361 catgcgcgct tcaatgtgcc tggaacatgg cgggctgggt cggacgccgt gcgtcagatc
    39421 ctgcgccagc aagccggtgt cgatattggt aacaccatta ttgccgatgg ttcagggctt
    39481 tcgcggcata acctgattgc ccccgccacc atgatgcagg tgctgcaata cattgcccaa
    39541 cacgacaatg aacttaactt tatctccatg ctgccgcttg cgggctatga cggctctttg
    39601 cagtaccgtg caggtctgca tcaggcgggc gtggatggca aagtctcagc gaaaaccggt
    39661 tcgttgcagg gggtatataa cctggcggga ttcattacca cagcgagcgg gcaacgaatg
    39721 gcatttgtac aatatctttc tggctatgca gtaaaacccg cggatcagcg taatcgccgt
    39781 attccgttag tgcgttttga aagccgtttg tacaaagata tttatcagaa caattagtca
    39841 aaaagaaacc ccggcgcatg gccggggttt cagattattg acaaagtgcg ctttgtttat
    39901 gccggatgcg gcgtgaacgc cttatccggc ctacaaaatc gtgcaaattc aatatattgc
    39961 aatcatcttg taggcctgat aagtgtagcg catcaggctt atttggcatt ggtcattagt
    40021 gattaacgct tgtaaatgaa ctcaacgcct tcttcgtcgt cttcgtccca gtcgtcatcc
    40081 cagtcttcat catcctcttc agcaatctct tcaagctgct gacggtgata atcatcccac
    40141 atgaattcga ctttctctgg ctgtttcgct tcttcagcct gcacgaccgg gttttcaatg
    40201 ataaaggtca tcacatccca gcagagatct ttcacgccca gtccgctcgc cgcagagatc
    40261 agataatatt tatcttccca gcccagcgct tcagcgatca ctttcgcttt ctcttcggct
    40321 tctgccttat ccagcagatc gatcttgttg aagactaacc aacgcggttt cgccgccaga
    40381 tcctggctgt atttttccag ctcgctgata ataatacgcg cgttttcaac cggatcggtg
    40441 ccgtcaatcg gatcgatatc gatgaggtgc aacaggacgc ggcaacgttc caggtgcttc
    40501 aggaagcgaa tgcccagacc tgcgccttcc gcagcgcctt caatcagtcc tggaatatcg
    40561 gcaacaacga agctcttttc gttgtccatt cgtaccacac ccagacttgg caccagagtg
    40621 gtaaacggat aatccgccac tttcggttta gccgccgata ccgcacgaat aaaggtcgat
    40681 ttacccgcgt ttggcatccc caacataccg acgtcagcca gcagcatcag ctccagcagc
    40741 agctcacgct tatcgcccgg cgtaccgtta gttttctgcc gtggtgtacg gttaactgac
    40801 gatttgaaac gggtattgcc cagaccgtgc cagccgccct tagcaaccag cagacgctga
    40861 ccgtgtttag tcatatcacc catggtttca ccagtaccct ggtcgattac acgcgtacct
    40921 accggcactt taatcgtcac gtctttaccg cgcttaccgg tacaatcgcg gcttgcgccg
    40981 ttctgaccgc gctctgcacg gaaagatttt tcaaaacgat aatcgataag cgtgttcagg
    41041 ttctcgtcgg cttccatcca tacgtcacca ccatcaccgc cgtcgccgcc atccgggccg
    41101 cctttcggaa tatacttttc gcggcggaag ctcacgcaac cattaccgcc atcacctgca
    41161 acgaccagaa tcgatgcttc atcaacaaac ttcattttat tctccgtaaa tcattcgcct
    41221 gcgcggggtt gcgataccac cgttgtatgc ttacgtaatc cgccccaaat acgatgacca
    41281 atggcggaat acatcgcgcc cgcaaccacg acaaacgcac cgagataacc taaaaggttt
    41341 aacatcggtc tggcgaagaa atcgggccag gccagtgata aaagatctga aaaaaacagc
    41401 gtaaacagtg gggtgagcgt gatgatcgcg ctcacctgcg ctgcctgcca gcgagccatc
    41461 gcttccgcca aggcgccata tcctaccaag gtattcagtc cgcaaaaaat taaacatgcg
    41521 agctgccagt ggctaagctg cgctatcact ccaggctttg ccagagggaa gagcgcgatt
    41581 gtacataaag tgtacagtaa aaacaggatc tgcggtgagg ccagccgacg caataaaacc
    41641 ttttgcgcca cgccataact cacccaaacc gtcgccgcac cgaccccaaa gataactccc
    41701 caggtgtaat cggtgagctt tgtaaatatc tcgaccagac tggtgttaaa aaacatcacc
    41761 agaccgctca ggagcatcaa tgcccctaca acctgagtgc tgcgcatttt ctctttcagg
    41821 ataaatacgc tggcaaccat catgccaact ggcgagagtt gcccaatcac ctgcgaagcg
    41881 gtcggactca ggtattgcaa ggatgagctg aacaggatga agttcccaaa cagcccggcg
    41941 gtcgccactg ccaacaaaat caaccagcgt ggcttacgaa acacgcgtaa tggcggcaac
    42001 ctcttcttca ccgcaagaat ggcaccaagg ccaatactcg ccatcaagaa acggtaaaac
    42061 acgattgtcg gaggttccat cacctccagc acctgcttca ttgcgattgg caacgccccc
    42121 cagcaaattg ctgtggtaag cgccaaaaga atgccaatgc ctgcctgctg cttcataccg
    42181 tattccctgc aaagcgcatt tgcggattaa cgtcgcacca tgcgcatttt taccggttat
    42241 cgaatgtaaa aagccccgca acgtgttgcg gggctttcat ccgttaccgg gacgcgaaaa
    42301 acttattcag cttcgatgct gataaattta cggtttttcg ggcctttaac ttcgaatttc
    42361 actttaccgt ctgctttagc aaacagagtg tggtcacgac cgcaacctac gttagcgcca
    42421 gcgtggaatt tggtaccacg ttgacgaacg atgatgctac ccgccagaac ggattcgcca
    42481 ccgaaacgct taacgcccaa gcgtttagct tctgaatcgc gaccgttacg tgtggagccg
    42541 ccagcctttt tatgtgccat ttgaaatctc tcctcaggtc ttaggcgctg atgccagtaa
    42601 ttttcacatc agtgaaccac tgacgatggc cctgctgctt acgatagtgt ttacgacgac
    42661 gaaacttaac gattttaact ttctcgccac gaccgtgagc aacaacttca gctttgatta
    42721 cgccgccatc aacgaaagga acgccgattt tgacttcttc accgtttgcg atcatcagca
    42781 cttcagcgaa ctcaacagtt tcgccagttg cgatgtccag cttttccagg cgaacggtct
    42841 gaccttcgct tactcggtgt tgtttaccac cactttggaa aaccgcgtac atataaaact
    42901 ccgcttccgc gcacaccttt tcaatgattc agagtgcgct ataaatattc acaatagggc
    42961 gcgaatatta cgcaaaacgc acgcctttga caagtgctac agtcaataca cgaagaaaaa
    43021 aacacaactt gtacggtaac gtttatctgt gccatttttt cagtacaatt accctatatt
    43081 cctaaccata aaccctaagt tgcctttgtt cacagtaagg taatcggggc gaaaagcccg
    43141 gcttttgcga tgaatttaga aaaaatcaat gagttaaccg cgcaagatat ggcgggtgtt
    43201 aatgcggcaa tccttgagca gcttaattcc gacgtccaac tgatcaatca gttaggctat
    43261 tacatcgtca gcggcggcgg taaacgtatt cgtccgatga ttgctgtact ggctgcacga
    43321 gctgttggct atgagggaaa tgcgcatgtc accattgctg ccctgatcga gtttatccac
    43381 acggcgactc tgctacacga cgacgttgtg gatgaatcag atatgcgcag gggtaaagct
    43441 accgccaacg ccgcatttgg caatgccgcc agcgtgctgg taggcgattt tatttatacc
    43501 cgcgcgttcc agatgatgac cagcctcggt tcactcaaag tgctggaagt catgtcagaa
    43561 gctgtaaacg tcatcgcaga aggtgaagtt ctgcaactga tgaacgttaa cgatccggac
    43621 atcactgaag aaaactacat gcgcgttatc tatagcaaaa ccgcgcgtct gtttgaggct
    43681 gccgcgcagt gttccgggat tctggctggc tgtacgccgg aggaggagaa aggcctgcag
    43741 gattatgggc gctatctcgg cactgctttc cagttgatcg acgatttact cgattacaat
    43801 gccgatggcg aacagttagg taaaaacgtc ggcgacgatc tgaacgaagg taaaccgacg
    43861 ctgccgctgc tgcatgcgat gcatcatggc acaccagaac aggcacagat gatccgtacc
    43921 gccatcgaac aaggtaacgg tcgccatctt ctggaaccgg ttctggaagc aatgaacgct
    43981 tgtggatctc ttgaatggac gcgtcagcgc gccgaggaag aagcagacaa agccatcgca
    44041 gcgttacagg tgctcccgga caccccttgg cgagaagcac tcatcggcct cgcgcacatc
    44101 gctgttcaac gcgatcgtta atcccctccc ttcatcccgc gcagtgcgcg ggatgattcc
    44161 agtaaattcc ataaaaacac taattcagcg ctataacacc tgaaaaaagg gcgataaatc
    44221 tttatatatt ctgaatattc acactcttta caggaacttt ttagcgcaat agaccatcag
    44281 gagtatagtg atgcccgaca gaagaagtgt tctgaatgaa agcgactaac ttaaggagtg
    44341 aggaaagatg gaaagtaatt tcattgactg gcatcccgct gacatcattg caggtttgcg
    44401 caagaaagga acgtcaatgg cggcggaatc tcgcagaaat ggtttgagtt cctcaacgct
    44461 ggcaaacgca ttatcgcgcc catggccgaa aggagagatg attattgcga aagccctggg
    44521 aactgacccc tgggttatct ggccatcacg ctaccatgat ccgcagaccc atgagtttat
    44581 cgacagaacg cagttgatgc gtagctacac taaaccgaaa aaatgagtgg tctggcggta
    44641 gccccgcgaa cggggctgcc agctctcaga cgattactcg cctttcacac gctcaatatt
    44701 tgcacctaaa gcgcgcagtt tgtcttcaat gcgttcgtag ccacgatcga tgtgataaat
    44761 acgatcaacc accgtcgtcc cttccgcaat acagccagcc agcaccaggc ttgctgatgc
    44821 acgcagatcg gttgccataa cctgtgcacc agaaagtttt tcaacaccat gacaaataac
    44881 ggtattgctt tcgatttcgg cgtgcgcgcc catacggctc agctctggca catgcataaa
    44941 gcggttttca aagaccgttt cggtgatgaa cccggtccct tcagccacca ggttcaacag
    45001 cgtgaactgg gcctgcatat cggtcgggaa tgccggatgc ggcgcggtac gtacgttaac
    45061 agccttcgga cgtttgccat gcatatccag gctaatccag tcttcgccga cttcgatgtc
    45121 cgctccagca tcacgcagtt tcgccagcac ggcgtccaga gtatctggct gcgcgttacg
    45181 gcagataatt ttgccacgag agatcgccgc cgccaccagg aaagtaccgg tttcgatacg
    45241 atccggcagc acgcgataga caccgccgcc taaacgttcc acaccttcga tgacgatacg
    45301 atcggtgccc tgaccgctaa ttttcgcacc cagcgtaatc aggaagttcg cggtatcgac
    45361 gatttccggt tcacgcgctg cgttttcaat aatcgtggtg ccttccgcca gggttgcagc
    45421 acacatgatg gtcaccgttg cgccaacgct gactttatcc atcacgatat gtgcgccttt
    45481 cagacgacca tcgacggaag ctttaacgta accttcttcc agtttgatgg tcgcgcctaa
    45541 ttgttcgagg ccagaaatgt gtagatcaac cggacgcgca ccgatcgtac aaccgccagg
    45601 tagtgaaact tgcccctgac caaagcgcgc taccagcggc cccagcgccc agatagaagc
    45661 acgcatggtt ttaaccagat cgtaaggtgc gcagaataca ttaacgtcgc gggcatcaat
    45721 atgcacagaa ccattacgtt ctactttcgc acccagctgg cttagcagtt tcattgatgt
    45781 atcgacgtct ttcagtttcg ggacgttctg gatctctacc ggttcttccg ccagtagtgc
    45841 ggcaaaaaga ataggcagag cagcattttt agcgccggaa attgtgactt cgccctggag
    45901 cttcgttggc ccctgaacac gaaatttatc catttagttt gttctcagtt aacaattcat
    45961 atccgctacc ggcgaatcgc ccatagctca aaagccgttc agtttgcgat cgcgcgccca
    46021 ctccgcaggg gtatacgctt tgatcgacac agcatgaatg cggttatccg caatatattc
    46081 catcagcggg ccatagaccg tctgctgttt tttaacccga ctcataccgt caaacaactc
    46141 acccacggca ataacctgaa agtggctgcc atcgccggaa acgtggactt cctggaggga
    46201 gagagcgttc atcaacacgc tctgaatttc attattttcc atgggatctt caatcatcag
    46261 ttaataaacc agcgaaacat cttagagcaa agttgcgctg gcataaataa gcaaaaagcc
    46321 tcgctgataa atcagacaag gctcgacttg caggcaggtt tgccggacag gcggttaacg
    46381 ccatatccgg cctgaaaaaa tttaacgagg cagaacatca gcaggcaaat tatacaattt
    46441 cgcgagggta tacactttgt cgtttacccc ctgaagcgtc acgttgttgc cctgcttttt
    46501 cgccagatcg ataagatgga gcagcagtgc cagtcccccc gtatccacgc gggacacacg
    46561 gctaagatcg atgcaggtaa tccccttcac cgcctcctca cgcatttccc aaagcggtag
    46621 cagaacgtcc tgatccagct ctccggataa cgccagcgtg tcacccgtct gcatccagct
    46681 cagtgactcg ctcattattt tttctcttcc agagtgattt tctgttgaga aatcgatttc
    46741 agttgcgcag tcaggccgtc gatacctttg gtacgcagca gcgttcccca ctcgttttgt
    46801 ttggtggtga tcatactgac gccttcagca atcatgtcgt aagcttgcca gtttcccgtc
    46861 tgggagtttt tacgccactg gaagtccaga cgcaccggcg gacggccatt cgggtcaata
    46921 atggtaacgc gaataggcac aatggtttta tcgcccagcg gctgttctgg cgcaatctga
    46981 taggtttgac cgtgatacat cgccagcgcc tgaccgtaag cctgcttcag gtactcacgg
    47041 aaagcggcaa agtaggcttc acgttgagca ggggtcgcac tcttgtaata ctggcccagc
    47101 accagcgcac cggcgtattt cacctgtacg tatggcagca gttcctgatc aacaatggta
    47161 cgcagataat ccgggttggc ccgaatttgc ggttgctcat tcttcaggcg atcgaacgtt
    47221 ttctgcgccg cctcgtccat cagcttataa ggattggtct ggtctgccgc ggttgccgca
    47281 ctcagaggtg caatcaccag caaagcgacc atcattaaac gtttaaacat gcgtcggttc
    47341 tcctgaaatt atttcgttgt acccacaggt tcagtggttt cattattacc tggcgcagca
    47401 gctggcgcat cgccactatt cttattgtca tcgcctttac taccgtaaag gaactgacca
    47461 atgagatctt ccagcaccat cgcagacttg gtgtcctgaa ttgtatcgcc atccttcagg
    47521 atagcagtcc ccagttccgg gtcttcaaaa ccgacgttta atgccagata ttgttctccc
    47581 agcaggccgg aagtacgaat gctcagcgaa ctggtatcag gaatgtggtt ataacgttgt
    47641 tcaatttcca gcgttacgcg cggcagatag gttttcgggt ccagcgtaat atccgccacc
    47701 cggcccacaa caacaccacc aatgctgacc ggagagcgcg ctttcaggcc gccaatgtta
    47761 tcgaacgtcg cataaagcgt gtaggtcgat tcagtacgta tggacgtcac gttcgccgcc
    47821 ttcaggcaaa caaacagcgc cgccagcagt gctgctaata aaaagatacc cacccaaatt
    47881 tcattttttt tcgtttgcat gaactcaatt cccaaacatc aatgcggtca gcacaaaatc
    47941 cagccccaga acagccagag acgagtggac aacggtgcga gtggttgccc ggctaatccc
    48001 ggcagacgtc gggatggcgt cgtagccgtt aaacaacgaa atccacgtca ccgtgatggc
    48061 gaacaccacg ctcttaatca gacagttgac cagatccata cgccagtcga cggcattttg
    48121 cattgccgac cagaagaacc cgctatcaat gcctttccaa ctgacgccga ccagcgatcc
    48181 gccccagatc cccacggcga cgaagataac cgtcaacagt ggtaatgaaa taaccccagc
    48241 ccagaaacgg ggagaaataa cccgacgcag cggatccacc gccatcatct ccatactgga
    48301 gagttgctct gtagcgcgca tcaggccgat ttctgcggtt aacgccgaac cagcacgccc
    48361 ggcaaacaac agcgcggcaa ccaccggccc cagttcacgc agtagcgata acgccaccag
    48421 catacccaga ctggtttccg cgctataagt ggtcagaacc agataacctt gcagccccaa
    48481 caccattccg atgaacacgc cagaaactac aataatcagc atcgacagga cgccgacatt
    48541 atagagctgg cgcaccagca gcggcgcatg tttgcgaaat tccggtttgc cgaccagcgc
    48601 attgaataac attaacccgg cccgcccgaa cgttctcagg gttttaatcc ctttatgtcc
    48661 gagcgacgcc agcgcattta acagcatgag tggcttaact ccctggtaaa agatcagcgt
    48721 gataatcgcc ggcaggatag cggaacggaa caggcccgtc agctatcccg tccagaaact
    48781 gacgtacgcg cggatcagga ttcgtctgca acgcctgggc actgccatga gcgacaattt
    48841 ttttgtccgc caggatccag gcgtgatccg caatacttaa cacttccggc acatcgtgag
    48901 aaaccaccac acaagtcacg cccagcgcgc tgttcagctc agaaatcagc ttcaccagta
    48961 cgcccatggt gatgggatct tgcccaacaa aaggttcatc aaacatgatg agatccggct
    49021 ccagcgcaat cgcacgcgcc agcgctgcac gccgcgccat cccaccggaa agttcagaag
    49081 gcattagttt agccgctcca cgcagcccca cggcctccag cttcatcatc accgtactat
    49141 gcaacaatgg cgcgggaagt tgggtatgtt cgcgcagtgg ataggcgacg ttgtcaaata
    49201 cgttcatatc agtgaacaac gccccggact gaaataacat gctcatccgt ttacgcactg
    49261 tatacaggcg cgaacgagac atcgccggaa tattctcacc atcgaaaagg atctcaccat
    49321 gatctggtgc gatttgcccg ccaatcagac ggagtagcgt cgttttaccg atgcccgatg
    49381 gccccatgat cgccgtgatc ttccctcgcg gcacggtcag ggaaatatta tcgaagatgc
    49441 agcgattgcc acgcgtaaaa ctgacatcgc gcatatcgac taaattcgcc acagactgct
    49501 ccataattca cccttcgtct tgcgttgatt ttctaagcat ggcgctcaat ttaaccttga
    49561 acccaacata tttacagaat attacccgcc gtggttagcg aaagctggca tttgttttac
    49621 tttttagccg cataaagtca aaattaagaa tccgttacgg ctttctgaaa atcttcagcg
    49681 gaccggcgag tatacctgaa gaaaggacgt tagatgcttt tagctacggc actgttaatt
    49741 gttggtttac ttttggtcgt ttacagtgcc gaccgcctgg tttttgccgc gtctattctt
    49801 tgccgaacct ttggcatccc gccgctgatc atcggcatga cggtggtcag tattggtaca
    49861 tcgttaccag aaatcatcgt ctcgcttgcc gcgtctctgc acgaacaacg cgatttagcc
    49921 gttggtacag ccctcggctc aaacattatc aatatattgc tgatcctcgg tctggctgcg
    49981 ctggttcgtc cttttaccgt ccattccgat gttctgcgcc gtgaattacc cttaatgttg
    50041 ttggtcagcg tggtggccgg ttccgtactc tatgacggac aacttagtcg aagcgatggt
    50101 atctttctcc tctttctggc tgtgctatgg ctgctgttca ttgttaaact tgcacgtcag
    50161 gggactgaca gcctgaccag agagcagctt gcagagctgc cgcgtgacgg cggattgccc
    50221 gtcgcgtttt tatggctcgg cattgcgctt atcatcatgc cagtggccac gcggatggtg
    50281 gttgataacg ccacggtgct ggcgaattac tttgccatca gcgagttgac gatggctctg
    50341 acggcaattg ctatcggaac cagtctgccg gaactggcaa ccgcaatagc gggggttcgc
    50401 aaaggtgaaa acgacattgc tgtcggaaat atcattggcg caaacatttt taatattgtc
    50461 atcgtgttgg gtttacccgc gctgataacg ccaggagaga ttgatccact ggcgtacagt
    50521 cgtgactaca gcgtgatgtt gctggtgagc attatttttg cgttgctgtg ctggcggcgc
    50581 tccccgcaac cgggccgtgg tgtaggggta ttattaactg gcggatttat cgtatggctg
    50641 gcgatgttgt actggttatc gccaatactc gttgaataac tggaaacgca ttatgtcgca
    50701 cgtagagtta caaccgggtt ttgactttca gcaagcaggt aaagaagtcc tggcgattga
    50761 acgtgaatgc ctggcggagc ttgatcaata catcaatcag aatttcacgc ttgcctgtga
    50821 aaagatgttc tggtgtaaag ggaaagttgt cgtcatgggg atggggaaat cggggcacat
    50881 cgggcgcaaa atggccgcaa cgtttgccag caccggtaca ccttcatttt tcgtccatcc
    50941 tagtgaagcc gcgcatggtg atttaggcat ggtcacccca caggatgtgg tgattgctat
    51001 ctctaactca ggtgaatcca gcgaaatcac ggccttaatt ccagtgctta agcgtcttca
    51061 cgtaccgtta atctgcatca ccggtcgccc ggagagcagc atggcgcgcg ccgcagatgt
    51121 gcatctgtgt gttaaagtag cgaaagaagc ctgtccgtta gggctggcac cgaccagcag
    51181 caccaccgcc acgctggtta tgggcgatgc cctcgctgtc gcgctgttaa aagcacgcgg
    51241 ctttactgct gaagattttg cgctctcaca cccaggcggc gcactgggtc gtaaacttct
    51301 gctgcgcgta aacgatatta tgcatacggg cgatgagatc ccgcatgtta agaaaacggc
    51361 cagtctgcgt gacgcattgc tggaagttac ccgcaaaaat cttggtatga ctgtcatttg
    51421 cgatgacaat atgatgattg aaggcatctt taccgacggt gatttacgcc gtgtcttcga
    51481 tatgggcgtg gatgttcgtc agttaagtat tgccgatgtg atgacgccgg ggggaatacg
    51541 tgtgcgccct ggcattctgg ccgttgaggc actgaactta atgcagtccc gccatatcac
    51601 ctccgtgatg gttgccgatg gcgaccattt actcggtgtg ttacatatgc atgatttact
    51661 gcgtgcaggc gtagtgtaaa gattcaagga taaacaacaa tgagcaaagc aggtgcgtcg
    51721 cttgcgacct gttacggccc tgtcagcgcc gacgttatgg caaaagcaga gaacattcgt
    51781 ctgctgatcc tcgacgttga tggcgtactg tcagatggcc tgatttatat gggcaataac
    51841 ggcgaagagc tgaaagcgtt caatgttcgt gacggttatg gcattcgttg tgcgctcacc
    51901 tctgatattg aagtcgctat cattaccggg cgaaaggcta aactggtaga agatcgttgt
    51961 gccacattgg ggatcactca cttgtatcag gggcagtcaa acaaactgat cgcctttagc
    52021 gatttgctgg aaaaattggc gattgccccg gaaaatgtgg cttatgttgg cgatgatctc
    52081 atcgactggc cggtaatgga aaaagtgggt ttaagcgtcg ccgtggccga tgcgcatcca
    52141 ctgttgatcc cgcgcgccga ttacgtgacg cgcattgctg gcggtcgtgg cgcagtgcgc
    52201 gaagtttgcg acttattact cctggcgcag ggcaaactgg atgaagccaa agggcaatcg
    52261 atatgagtaa agccagacgt tgggttatca ttgtgctatc actggcggtt ctggtgatga
    52321 tcggcattaa tatggccgaa aaagacgata ccgcccaggt ggtcgtcaac aacaatgatc
    52381 ccacctataa aagcgagcat acggacacgc tcgtctataa cccagaaggg gcactaagct
    52441 atcgattgat tgctcaacac gttgaatatt attccgatca ggccgtttcg tggtttacgc
    52501 agccggtact taccacgttt gataaggata aaatcccgac atggtccgta aaagcagata
    52561 aagccaagct gaccaatgac cggatgctct atttatatgg acacgttgaa gtcaacgcac
    52621 ttgtgccaga ctctcaactt cgcagaatca cgacggataa cgcgcagatc aatctggtga
    52681 cgcaggatgt tacctctgaa gacctcgtca cgttatacgg aacaacattt aactccagcg
    52741 gtctgaaaat gcgcggcaac ttacgcagca agaacgccga gctgattgaa aaggttagaa
    52801 catcctatga aattcaaaac aaacaaactc agccttaatc ttgtgcttgc cagctcactt
    52861 ctggccgcca gcattccggc atttgccgta accggagaca ctgatcagcc gatccacatt
    52921 gaatcggacc agcaatctct tgatatgcaa ggcaacgtgg ttacctttac cggtaatgtc
    52981 atcgtcaccc agggcaccat caaaattaat gccgacaaag tggtcgttac ccgtccgggc
    53041 ggcgaacaag gtaaagaagt gattgacgga tacggtaaac cggcaacgtt ctaccagatg
    53101 caggacaacg gtaaacccgt tgaaggtcac gcttcccaga tgcactacga actggcaaaa
    53161 gattttgtcg ttctgacggg taatgcttat ctgcagcagg tcgatagcaa cattaagggc
    53221 gataagatca cttacctggt gaaagagcag aaaatgcagg ctttcagcga caaaggcaag
    53281 cgcgtaacca ccgttctggt gccgtcgcag ctgcaggaca aaaacaacaa aggccagacc
    53341 ccggcacaga agaagggtaa ttaattcgtt atggcaacat taactgcaaa gaaccttgca
    53401 aaagcctata aaggccgtcg cgtggtagaa gacgtcagcc tgaccgtcaa ctccggggaa
    53461 attgtcggtc tgctggggcc aaacggtgcc ggtaagacca ccactttcta catggttgta
    53521 ggcattgtgc cgcgcgatgc gggcaacatc attattgatg atgacgatat cagtctgctg
    53581 cctctgcatg cacgcgcgcg ccgcggtatc ggctatctgc cacaggaagc ctccattttc
    53641 cgtcgcctca gcgtttacga taacctgatg gcggtactgc aaattcgtga cgacttgtct
    53701 gctgaacaac gtgaagaccg cgcgaacgag ctgatggaag agtttcacat tgagcacctg
    53761 cgtgacagca tggggcagtc actctccggg ggtgaacgtc gccgtgtaga aattgcccgc
    53821 gcactggctg cgaatccgaa atttattctg ctcgacgaac cgtttgccgg ggttgacccg
    53881 atctcggtta tcgacattaa acgcatcatt gaacacctgc gcgacagcgg cctgggcgtg
    53941 ctgatcactg accacaacgt gcgtgaaaca ctggcggttt gtgaacgcgc ttatatcgtc
    54001 agtcaggggc atttgatctc ccacggcacg cctacagaaa tcttacaaga cgaacacgtt
    54061 aagcgtgtat accttgggga agacttcaga ctctgatagg gtagaagttt gcgacgtttt
    54121 agcaggagag tacgattctg aacatgaagc aaggtttgca actcaggctt agccaacaac
    54181 tggcgatgac gccacagctc caacaggcaa ttcgtctgtt gcagttgtcg acgctggaac
    54241 ttcagcagga gctacagcag gcgctggaga gtaatccgct gcttgagcaa atcgacactc
    54301 atgaagaaat cgacacccgc gaaacgcaag acagtgaaac gctggacacc gccgacgcgc
    54361 tcgaacaaaa agagatgccg gaagagctgc cgctcgatgc cagttgggac accatttaca
    54421 ccgctggtac accatccggc accagcggtg actacattga cgacgagctg ccggtctacc
    54481 agggcgaaac gacgcagacc ttgcaggatt acctgatgtg gcaggtcgag ctgacaccgt
    54541 tttccgacac tgaccgcgct attgctacct ctatcgtcga tgccgttgat gaaaccggtt
    54601 atctgactgt cccgctggaa gatattctcg aaagtatggg cgatgaagag atcgacatcg
    54661 acgaggttga agccgtcctt aagcggatcc aacggtttga cccggtcggt gtagcggcaa
    54721 aagatctgcg tgactgcctg ctaatccaac tctcccaatt cgataagacc acgccgtggc
    54781 tggaagaggc cagattgatc attagcgatc atctcgatct gttagccaat cacgacttcc
    54841 gcactttaat gcgcgtcacg cgtctgaaag aagatgtgct gaaagaagcc gtcaatctga
    54901 tccagtcgct cgatccgcgc cccgggcagt cgatccagac tggcgaacct gagtatgtca
    54961 ttccagatgt gctggtgcgt aagcataacg gtcactggac ggtagaactc aacagtgaca
    55021 gcattccgcg tctgcaaatc aaccagcact acgcctcgat gtgcaataac gcgcgcaacg
    55081 atggtgacag ccagtttatc cgcagcaatc tgcaggatgc caaatggttg atcaagagtc
    55141 tggaaagccg taacgatacg ctactgcgcg tgagtcgctg tatcgttgaa cagcagcaag
    55201 ccttctttga gcaaggtgaa gaatatatga aaccgatggt actggccgat atcgcccagg
    55261 ctgtcgaaat gcatgaatcg acgatatctc gcgtgaccac gcaaaaatac ctgcatagtc
    55321 cacgaggcat ttttgaactg aagtatttct tttccagtca cgtcaatacc gagggcggcg
    55381 gcgaagcttc ctccacggcg attcgtgcac tggtgaagaa attaatcgcg gcggaaaacc
    55441 cagcgaaacc gttgagcgac agcaagttaa cctctttgct gtcggaacaa ggtatcatgg
    55501 tggcacgccg cactgttgcg aagtaccgag agtctttatc cattccgccg tcaaaccagc
    55561 gtaaacagct cgtttgaccc aaccgataag gaagacacta tgcagctcaa cattaccgga
    55621 aataacgtcg agatcaccga ggcactgcgc gaatttgtta cagctaaatt tgccaaactt
    55681 gagcaatatt ttgaccgaat caaccaggtc tatgttgttc tgaaagtgga gaaagtcacc
    55741 cacacctcag atgcaacact gcatgtaaac ggtggtgaaa ttcatgccag cgcggaaggt
    55801 caggacatgt acgctgccat tgatggctta attgataagc tggcacgcca gttgaccaaa
    55861 cataaagata aactgaaaca acactaattg tccgggcaat tagcatgtgc atggcggcct
    55921 gttgtgcggc acaacgggcc atttgtacgg ttaatgctcc gagcctgttc cactgtttga
    55981 gtgggcaggt tcttaggtga aattatgaca aataatgata caactctaca gcttagcagt
    56041 gttcttaaca gggaatgtac gcgaagccgc gtccactgtc agagcaaaaa acgcgccctg
    56101 gaaatcatca gcgagctggc ggcgaaacaa cttagcctgc cgcctcaggt ggtttttgaa
    56161 gctatcctga cgcgggaaaa aatgggcagt accggtatcg gcaatggtat tgccattccg
    56221 catggcaaac ttgaagaaga tactctgcgt gccgttggcg ttttcgttca gctcgaaacg
    56281 cctatagctt tcgatgccat cgacaaccaa ccggtggact tactttttgc cctgctggtg
    56341 ccggcagacc aaactaaaac gcacctgcat actctgtcgc tggtggcgaa acgtctggcg
    56401 gacaaaacca tctgccgccg tttacgcgca gcccagagcg atgaagagct gtatcaaatc
    56461 attacggata ccgaaggtac tccggatgaa gcgtagttat tcggtaatgt ctcttttaga
    56521 cgttgtgagg agaaacagta catggtactg atgatcgtca gcggacgttc aggttcaggt
    56581 aaatctgtcg ccctgcgtgc gctggaagat atgggttttt actgcgtgga taaccttccc
    56641 gtagtgttgt tacccgatct ggctcgaact ctggccgatc gcgagatttc tgccgccgtc
    56701 agcattgatg ttcgtaatat gccggagtca ccagaaatat tcgaacaggc gatgagtaac
    56761 ctgcctgacg ctttctcacc gcaactactg ttcctggatg ccgaccgtaa taccttaatt
    56821 cgtcgttaca gtgacacgcg ccgactgcat ccgctttcca gcaaaaacct gtcgctggaa
    56881 agtgctatcg acaaagaaag cgatttgctg gagcctctgc gttcgcgagc ggatctgatt
    56941 gtcgatacct cagaaatgtc cgttcacgag ctggctgaaa tgctgcgtac ccgtctgctg
    57001 ggtaaacgtg aacgcgaact gaccatggtc tttgagtctt tcggcttcaa acacggtatc
    57061 cctatcgatg cagattacgt ctttgacgtg cgcttcttgc cgaacccgca ctgggatccg
    57121 aaactgcgtc caatgacagg tcttgataaa cctgtcgccg cgttcctcga ccgccacaca
    57181 gaagtacaca attttatcta ccagacgcga agctatcttg agctatggtt acctatgctg
    57241 gaaaccaaca accgtagcta cctgacggtc gccattggtt gtaccggcgg gaagcaccgt
    57301 tcggtgtata ttgcagagca actggcagac tacttccgct cgcgcggtaa aaacgtccag
    57361 tcacgccatc gtacgctgga aaaacgtaaa ccatgaccgt caagcaaact gttgaaatca
    57421 caaacaagct gggcatgcat gcccggcctg caatgaagct gtttgaatta atgcagggtt
    57481 ttgacgctga agtgctgtta cgtaatgacg aaggcaccga ggctgaagcc aacagcgtta
    57541 ttgcgctgct gatgttggat tctgccaaag gacggcagat tgaagttgaa gcgaccggtc
    57601 cacaggaaga ggaagcactg gccgccgtta tcgccctctt taattctggt tttgatgaag
    57661 attaatcttc atcactttga catacaactc ccttcaagct cccccgacaa taagaaaatc
    57721 acgtactgaa atcgttctca atcaacgtca tttgtacatt ttgtgcgctt ttcactttca
    57781 gaagaacctt aagaaaacct taagaggcat tgtttaggtt ttgtttaagt taatcgacca
    57841 tactggagat cgtcagaaaa tatttccagg agatggcatg attcgcttat ctgaacaaag
    57901 tcccctgggc accggaagac atcggaaatg ttatgcgcat ccggaagatg cccaacgctg
    57961 tatcaagatt gtctaccatc gtggtggcga taaagagatc cgccgtgagt taaagtacta
    58021 cgcgcatctt ggtcgccggt taaaagactg gagtggaata ccgcgctatc acggtaccgt
    58081 tgagacggat tgcggaaccg gttatgtcta cgatgtgatt gccgattttg acggcaagcc
    58141 ttccattacg ctgaccgaat ttgctgaaca atgccgttac gaagaagaca ttgcacaact
    58201 gcgccagcta ctgaaacagc tgaagcgtta tttgcaggat aaccgtatcg tgacgatgtc
    58261 gctgaagccg cagaatatcc tctgtcaccg catcagtgag tcagaggtta tcccggtcgt
    58321 ctgcgacaac attggtgaaa gcacattgat tccactggcg acctggtcga agtggtgttg
    58381 tctgcgtaag caagagcggt tatggaaacg atttatcgcc caaccggcgc tggctattgc
    58441 cctgcagaaa gatctgcaac ctcgtgagag caaaacgctg gccctcactt cgcgcgaagc
    58501 ttaatccagc tggtgttgtt gcataaacgg ctcaccgcct aactgataca tctgccgtaa
    58561 aatccacgcc tgacggctac gcacgtagcc cgatggcgag gagactttga aacgaagtgg
    58621 attaggtaat acagctgcca gtaatgcagc ttccgaccgg gtaagtttgc tcgcgggttt
    58681 gtggaaataa cgttgtgccg cagcttcgac gccaaacaca ccgtcgccaa attcggcgat
    58741 attcaggtaa accgtcagga tacgcttttt gctccagact gtttctatcc ccagcgttaa
    58801 tccagcttcc agcccttttc gtacccagct acgcccatcc cataaaaaga gattttggcc
    58861 gtctgctgag aaatcgttga agcaccgcga atacggtttt cattgcgctc gttgtgcgcc
    58921 agggctttct caatggaagc gacatcaaag ccccagtgct caggaaattt ctgatcttct
    58981 gcggcaatca ccgccagtcc catccacggc gagatttgat ccatactgac ccagtcagaa
    59041 tgtgccacgt aacgaaaatt gccatgcagc caggcgctga cctgtcgctc gaccattacc
    59101 gctgagaagg gaacaggcgc aacgctaaac aacgcgatgc ccccgcccca gaaaacggcg
    59161 aggacaacca ttaaccgcaa aagaaaacgg cgaacgaaac taaacaccgt taagcggctt
    59221 ttactcattc agccagaacc agcacgcggg aaaccagctt atcaatgccg ctcgccgctt
    59281 ctgcaatgtt ctgcgccagc atatatgctg gggtggtgac aatcttattg tcttcatcaa
    59341 ccacgatatc atcgacagga cacggcacat gctccgcgcc catctcttcc agcacttctg
    59401 cggtatcgat atcagtaccg atggtcaaac gcagcgggaa atcgaaaatt ttcggcagca
    59461 tcgccggggc aatacacata aaaccaagcg gttttccggc ctgatgcatc gcttgtgcca
    59521 gcgcctttaa ttcacggtca acggtgcatt cgctaccaag actggcaaaa ttgcttaaat
    59581 tcttcgccgc gccaaccccc cccggcacaa tcaacgcatc cagttcagcg gcatcggcct
    59641 gggccagagg acggatttca ccgcgcgtta ttcgtgccgc ttcaatcagc acattgcgcg
    59701 tttccgtcat cgcttcgcca gttaaatggt tgataacatc aacctgctgc ttatccggtg
    59761 caaagcagac cgcctgcgca ccgctgcgtg aaatagctaa caacgtcaac accgcttcat
    59821 gaatttcaga accgtcatag acgccgcatc cgctcagaat tacgccaatt ttcttcattg
    59881 tgatcatcct ttcgcaattg actgaaacac attaattttt ttaataaaaa tggtacgcat
    59941 cacacattta actgattcat gtaacaaatc atttaagttt tgctatctta actgcgtgcg
    60001 gcctgaaaaa cagtgctgtg cccttgtaac tcatcataat aatttacggc gcagccaaga
    60061 tttccctggt gttggcgcag tattcgcgca ccccggtcta gccggggtca ttttttagtg
    60121 gcttttgcca cccacgcttt cagcacttct acgtcgtgac gccactcttc tttcatctct
    60181 tcaatccatt caccgacgtt atcttcccag gcgggaaggt caggagactg aatttgctga
    60241 cccagctgtt gcagatggcg taaccccact gaccccgccg cacctttaat tttatgtcct
    60301 tcctcaacaa tgcctttttt atcctgcgcc gtcagattca actccagcac gctgacatag
    60361 cccggcatca tcttctcaaa caccgctaac ccgtcggtga tcagcttcgg tcctacaagt
    60421 tcgagatact gttccagcat gggaatatcg agcaatgctt ctgatttact gttctcttct
    60481 gtcgtcaccg tactctcctc atcatcctgg gtatcccaga atttcttgat catcgcggtt
    60541 agcgccggaa ccgaaagcgg cttactcagc acatcatcca ttccagcatt gaggtactct
    60601 tgtttgtctt tcagcacgtt agcggttaag gccaccagcg gcggtaaatc ctcgcgcgga
    60661 taacgcttcg tcagttcacg agagatatcc agcccggtca tatctggcaa ctgaatatcc
    60721 agcaacacca ggtcgtattc gcccggttta aacatctcca gcgccgcctt gccggtcatg
    60781 gcgacatcaa cgctgttacc taatttttcc agcacagaac gcgcgacaat cacgttcagt
    60841 tcaatgtctt ccaccagcag cacattcagc gccggtaaag gcatatcgtc ttcatcaaac
    60901 gcatcatcga cctcttctgc caccgacggt gcgtggatcg tcaacgtaaa ggttgaacct
    60961 ttgccctgtt cactggtaac cgtaatatcg ccgcccatat ttttcgccag acgacgagaa
    61021 acggccagac caataccggt gccggtggca ggtttaccgc catgactgtc tttcacctgg
    61081 taatacatgg cgaagatttt atccagctca tcctgcggaa tgccgatacc agaatcttcc
    61141 acttcaaaat gcagcatatc gccttcatcg tagcgcacgc gcacggtaac ctggccttgc
    61201 tgggtgaatt tgacggcgtt actgatgagg ttccacagga tctgccgtaa acgcgtcccg
    61261 tcggtaatga cctgatgcgg taatggcagc gtaggctcca ggttaaagcg caatcctttt
    61321 tgttgcgcct gcaatgcgga gagattttcc agatcggcaa ggaagctggt gaaatcaacc
    61381 ggctgattat caagctggac cttgcgccgt tccatcttat ccatgtcgat aatatcgtta
    61441 aagatattcc ccagcgtgac ggccgaaaca tggatagttt tgagatattt ttcctgctcg
    61501 gcggtgagtt cggtatccag cagaatgcgg ctcaggccga cgataccgtt cagcggtgta
    61561 cgcaattcgt gactgatggt ggaaataaac gtcgttttgt cgcggctggc gcgttcaagc
    61621 gcatcctgat accgcttacg ctcggtaatg tcgcgaccaa agcccatcaa accgtgacgt
    61681 ttacccacgc ggtcgtagta cggcacttta cggatttcaa agcaggcttt gcgcccgtcc
    61741 gggtaatcca gccactgttc ataggtcagt gacacattat gacggaacac tttttcatcg
    61801 gtttcaatga cttttgcggc ggcttccggt gagtaaacat cagcaggttt caggtgaacc
    61861 agttgttttt cgctttttcc ggtcagcagc tccatcgcgc ggttacagcc ggaaaactct
    61921 ttatcttcgt tacgataaaa aaccaagtcg ggtgaagcat caaggaagga acgtaagaat
    61981 gaggattgct gctcgagctg aatttgtgtc tcttcgcgct ctttgatttc aattttcagt
    62041 tggccgaagg tttcctgcag ttctgcttcc gctttttcac gaacggcaat ttcctgattt
    62101 agctgggcaa tattatcttt taactgaacg ttgaggctca aatcgcgctc gcgcatctcc
    62161 tccagttttt gcaccagccg tgacagacgt tgtcgtgact cctccagttg ctcgacgacc
    62221 accgatagaa agtagaccgc ccacggcgta atcagcaaac caaagaagat agaacgaata
    62281 acatcaatgc tttcgacctg accatgcagc accatggtta ccgccatttg taccacaatg
    62341 gcaagaacga cgagggccag cgccagcaac attgagaagc gcaccagacc taacttcatc
    62401 atcaggtcaa cataatactg cgccagcaga cgaatttgct tcattaggga attccttcac
    62461 gacaacctgc cccaataata cccaattctg acagttacgt tataaattgt gtgagaaatg
    62521 cggaattccc ctcaacgacc tactccgttg gaggtagcca cggacgtccc agcgccgatc
    62581 cctgtacgcc atgttcattc agatagcgat ccagctcgac cattcccgtc catcgatttt
    62641 cgcaccacag cggagcaagc agcgtcgggc gacgggcgct ggcggaaatg cggtggtaga
    62701 tcacttccgg tggcgtatgg cgaatcattt ctccggcagt gagcgtataa tcctccagtt
    62761 caataccgtt caaacgcacc gcttcccagg ctttcgccat aatgctgcct ttcacaatat
    62821 gcagcggatg cagctttatg ccgtccacgc tggtttcaac cactcgctcc agcgtttgca
    62881 aacattcagc ctgcccttcg ccaggcaggc cgacaattaa gtgggaacac accttcagcc
    62941 cgcgctgacg tgccagctgg gttgtacgct gataacaggc aaaatcatgg ccgcggttga
    63001 tgcgatgcag tgttttgtcg tgggcggttt gtagccccag ctccagccac acttcgtaac
    63061 cctggtcctt atattcgcaa agcagatcca gcaccgcatc cggcacgcag tccgggcggg
    63121 taccaacaca caaaccgaca atattggcct ggctcaccgc ctgctgatac atagaacgca
    63181 gcacctgaac ttccgcaaag gtgctggtat acgcctgaaa gtaggccaga tagcgtttag
    63241 cgcggttaac taaattcgcc tggtgcgcca gttgctcggc aatggaacga tgctgctgcg
    63301 cttcatcggc aaacgaggca acattacaga atgtgcagcc accacgcccg atggtaccgt
    63361 cacggttagg gcagctaaaa ccgccgtgca gcgtcagctt atgcaccttt tgcccataac
    63421 gacgggtgag atcaccacca aacatattga ctaatttctg taactgcata atctgataga
    63481 ccgcgccttg aaaagaggcc aaagcctgcc atttttagct caattcggcg atgacctggg
    63541 tcaatcgtcc cggcctgctt ttatcaactg cataatcaat caaaattacc gaaatttcat
    63601 gcacaatcac ataaatcact tttgcttatc ttgtgtcaga tttttttatc tccttatgga
    63661 ttttaggcaa aaacagtagc atgaaacgtc attaccaatt aaggcagtat aaaatgctgg
    63721 ttttctcgtc agttcaaggc aggataaggg ttaacacgcc tttatgacag tcaggaattg
    63781 actgtttctc taacgactct ccttttagcc ttaaagataa aatctatttt aatttcagct
    63841 atttaataaa gaattttgcg ctaaagcaca tttctgtacc aataagcttg ccatttgacc
    63901 tgtatcagct ttcccgataa gttggaaatc cgctggaagc tttctggatg agcagcctgc
    63961 tcatcatatt tatgcagtaa ttgagatccc ctcttcaccg tattaaccga tgcgaaaagg
    64021 acaacaaggg ggcgaatgcg aggcgcgcgt atgacacgca aaccccgtcg ccacgctctt
    64081 tctgtgcccg tgcgcagcgg ttcggaagtg gggttcccgc agagcctggg ggaggttcac
    64141 gatatgttgt acgataaatc ccttgagagg gataactgtg gtttcggcct gatcgcccac
    64201 atagaaggcg aacctagcca caaggtagtg cgtactgcaa tacacgcact ggcccgcatg
    64261 cagcaccgtg gcgcgattct cgccgatggt aaaaccggcg acggttgcgg cttgctgtta
    64321 caaaaaccgg atcgcttttt tcgcatcgtt gcgcaggagc gcggctggcg tttagcaaaa
    64381 aactacgctg tcgggatgct cttcctgaat aaagatcctg aactcgccgc tgccgcacgc
    64441 cgcatcgttg aagaagaact gcaacgcgaa accttgtcga ttgtgggctg gcgtgatgtc
    64501 cccactaacg aaggcgtgct gggtgaaatc gccctctcct ctctgccacg cattgagcaa
    64561 atttttgtga acgccccggc aggctggcgt ccacgcgata tggagcgccg tctgtttatc
    64621 gcccgccgcc gcattgaaaa gcgtctcgaa gccgacaaag acttctacgt ctgtagcctg
    64681 tcgaatctgg tgaacatcta taaaggtctg tgtatgccgg cggatctgcc gcgcttttat
    64741 ctggatcttg cggacctgcg tctggaatcg gccatttgcc tgttccacca gcgcttctcc
    64801 actaacaccg taccgcgctg gccgctggcg caaccgttcc gctatctggc gcataacggt
    64861 gaaatcaaca ccatcaccgg taaccgccaa tgggcgcgtg cgcgtaccta taaattccag
    64921 acgccgctga tccctgacct gcacgacgcc gcgccattcg tcaacgaaac cggttctgac
    64981 tccagttcca tggataacat gctggaactg ctgctggcag gcgggatgga tatcatccgc
    65041 gccatgcgcc tgttagtgcc acccgcctgg cagaacaacc cggatatgga cccggaactg
    65101 cgtgccttct ttgactttaa ctccatgcat atggagccgt gggatggtcc ggcgggcatc
    65161 gtgatgtccg atggtcgctt cgctgcctgt aacctcgacc gtaacggtct gcgtccggca
    65221 cgctacgtca ttaccaaaga caagctcatc acctgcgcct ctgaagtcgg tatctgggat
    65281 taccagcctg acgaagtggt cgaaaaaggc cgcgtcgggc caggcgaact gatggtgatc
    65341 gacactcgca gtgggcgtat tctgcactcg gcagaaaccg atgacgatct gaaaagccgc
    65401 cacccttata aagagtggat ggagaaaaac gtccgccgac tggtaccgtt tgaagatctg
    65461 cccgatgaag aagtgggtag ccgcgaactg gacgacgaca cgcttgccag ctaccagaaa
    65521 cagtttaact acagcgcgga agagctggac tccgtaattc gcgtactggg cgaaaacggt
    65581 caggaagcgg tcggttcgat gggcgatgat accccattcg ccgtgctctc cagtcagccg
    65641 cgcattattt acgactactt ccgccagcag tttgcccagg tgactaaccc gccaatcgac
    65701 ccgctgcgtg aagcgcatgt tatgtcgctc gccaccagta tcggtcgtga aatgaacgtc
    65761 ttttgcgaag cagagggcca ggcgcaccgt ttaagcttta aatcgccgat tctgctctac
    65821 tccgatttca aacagctcac gacgatgaaa gaggagcact accgcgcaga tacgctggat
    65881 atcacctttg acgtcactaa aaccacgctc gaagcgacag tcaaagagct atgcgacaaa
    65941 gccgaaaaaa tggtacgtag cggcaccgtg ttgctggtgc tctccgaccg gaatatcgct
    66001 aaagatcgcc tgccggttcc ggctccgatg gcggttggcg cgatccagac ccgtctggtc
    66061 gatcaaagcc tgcgttgcga tgccaacatc atcgtcgaaa ccgccagcgc ccgcgatccg
    66121 caccacttcg ccgtgttgct gggtttcggc gcgacggcta tttatccgta ccttgcctat
    66181 gaaacgctgg gccgcctggt agacacccac gcgattgcca aagattatcg taccgtgatg
    66241 ctcaactacc gtaacggcat caacaaaggc ttgtacaaaa tcatgtccaa aatgggcatc
    66301 tccaccatcg cctcttaccg ctgctcgaaa ctgtttgaag cggtcggtct acacgatgat
    66361 gtagtgggcc tgtgcttcca gggcgcggtc agccgcattg gtggggcaag ctttgaagac
    66421 ttccagcagg atctgctgaa tctgtcgaaa cgtgcctggc tggcgcgcaa gccgattagc
    66481 cagggcggcc tgctgaaata cgtccacggc ggcgaatacc acgcctacaa cccggacgtg
    66541 gtgcgcacgc tgcaacaggc ggtacaaagc ggcgagtaca gcgactatca ggaatacgcg
    66601 aagctggtta atgagcgtcc ggcaaccacg ctgcgcgatc tgctggcaat tacgccgggt
    66661 gaaaacgcgg tcaacattgc tgatgttgaa ccggcaagcg aactgtttaa acgctttgat
    66721 accgccgcga tgtctatcgg cgcgttaagc ccggaagccc acgaggcgct ggcggaagcg
    66781 atgaatagca tcggcggtaa ctcgaactcc ggtgaaggcg gcgaagaccc ggcgcgctat
    66841 ggcactaaca aagtgtcgcg catcaagcag gtggcttctg gtcgcttcgg cgttactccg
    66901 gcgtatctgg tcaatgccga cgtcattcag attaaagtcg cccagggcgc gaagccgggc
    66961 gaaggtggtc agttgccggg cgacaaagtc actccttaca tcgccaaact gcgctattcg
    67021 gtgcccggag tgacgctgat ctccccgccg ccgcaccacg atatctactc tatcgaagac
    67081 ttagcgcagc ttattttcga cctcaagcag gttaacccga aagcgatgat ctccgttaag
    67141 ctggtgtccg aaccaggcgt tggcaccatc gcgactggcg tggcgaaagc ttatgcggac
    67201 ttgatcacca tcgcgggcta tgacggcggc accggcgcaa gcccgctgtc gtcagtgaaa
    67261 tatgcaggct gcccgtggga actcggtctg gtggaaaccc aacaggcgct ggttgccaac
    67321 ggcttgcgtc ataaaattcg tttgcaggtt gatggcggcc tgaaaacggg tgtcgatatc
    67381 atcaaagcgg cgattctcgg cgcagaaagc ttcggcttcg gcactggccc aatggtggcg
    67441 ctcggctgta aatatctgcg tatttgccac ctgaacaact gcgccacggg tgtagcaact
    67501 caggatgaca aactgcgtaa gaatcactat cacggcctgc cgttcaaggt gactaattac
    67561 tttgagttta tcgcccgtga aacccgcgag ctgatggcac agctgggagt aacgcgtctg
    67621 gtggatctga ttggtcgcac cgacctgctg aaagagctgg acggtttcac cgccaaacag
    67681 cagaaactgg cgctgtcgaa gctgctggag actgccgagc cacatgcagg taagtcactc
    67741 tactgcactg aaaacaaccc gccgtttgat aacggcctac tgaacgcgca gttgctgcaa
    67801 caggcgaaac cgtttgtcga tgaacgccag agcaaaacct tctggttcga tatccgcaac
    67861 accgaccgtt ctgtcggcgc gtcgctttca ggctatatcg cccagacgca cggcgatcag
    67921 gggctggcag ccgatcctat caaagcgtac ttcaacggca ccgcaggcca gagtttcggt
    67981 gtgtggaacg cgggcggcgt ggaactgtac ctgaccgggg atgccaacga ctatgtcggt
    68041 aaaggcatgg cgggcggctt aatcgccatt cgtcctccgg ttggttccgc cttccgcagc
    68101 catgaagcaa gcattatcgg caacacctgc ctgtatggcg cgaccggtgg tcgtctgtat
    68161 gccgcaggcc gcgcgggtga acgtttcggc gtgcggaact ccggtgctat caccgtggta
    68221 gaaggcattg gcgacaacgg ctgtgaatat atgacgggcg gtattgtctg tattctgggt
    68281 aaaaccggcg ttaacttcgg cgcgggcatg accggcggct tcgcttacgt tctcgatgaa
    68341 agcggtgatt tccgcaaacg cgttaacccg gaactggtcg aagtcttaag cgtcgacgat
    68401 ctggcgatcc atgaagagca tctgcgcggt cttatcaccg agcatgttca gcataccggt
    68461 tctcagcgcg gtgaagagat tctggcgaac tggtcaacct tcgccacgaa attcgcgctg
    68521 gttaaaccga agtccagtga tgtaaaagca ctgctgggtc accgtagtcg tagcgcagca
    68581 gagttgcgcg tgcaggcgca gtaaggggta gcaacaatga gtcagaatgt ttatcaattt
    68641 atcgacctgc agcgcgttga tccgccaaag aaaccgctga agatccgtaa aattgagttt
    68701 gttgaaattt acgagccgtt ttccgaaggc caggccaaag cgcaggctga ccgctgtctg
    68761 tcgtgcggca acccatactg cgagtggaaa tgcccggtac acaactacat cccaaactgg
    68821 ctgaagctcg ccaacgaggg gcgtattttt gaagcggcag aactgtcgca tcagaccaac
    68881 acactgccgg aagtatgcgg acgtgtctgc ccgcaagacc gtctgtgcga aggttcctgc
    68941 accctgaacg atgagttcgg tgcggtgacc atcggcaaca ttgagcgtta tatcaatgat
    69001 aaagcgttcg aaatgggctg gcgtccggat atgtccggcg tgaagcaaac cggtaaaaaa
    69061 gtggcgatta tcggtgcagg cccggcaggt ctggcgtgtg cggatgtcct gacgcgtaac
    69121 ggcgtaaaag ccgttgtctt cgaccgtcat ccagaaattg gcgggctgct gaccttcggt
    69181 attccggcct tcaagctgga aaaagaggta atgacgcgtc gccgtgaaat cttcaccggc
    69241 atgggtattg aattcaaact caataccgaa gtgggccgcg acgtacagct ggacgatctg
    69301 ctgagtgatt acgatgccgt gttccttggc gttgggactt atcagtcaat gcgcggcggg
    69361 ctggaaaacg aagacgccga tggcgtgtac gcagcgctgc cgttccttat cgccaacacc
    69421 aaacagttaa tgggctttgg tgaaacccgc gacgaaccgt tcgtcagcat ggaaggcaaa
    69481 cgcgtggtag tacttggcgg tggcgacact gcgatggact gcgtgcgcac gtctgtacgc
    69541 cagggggcaa agcacgttac ctgtgcctat cgtcgtgatg aagagaacat gccgggttcc
    69601 cgccgcgaag tgaaaaacgc gcgggaagaa ggcgtagagt tcaaattcaa cgtccagcca
    69661 ctggggattg aagtgaacgg taacggcaaa gtcagcggcg taaaaatggt acgtaccgaa
    69721 atgggcgaac cggatgccaa aggccgtcgc cgcgcggaga tcgttgcagg ttccgaacat
    69781 atcgttccgg cagatgcggt gatcatggcg tttggtttcc gtccacacaa catggaatgg
    69841 ctggcaaaac acagcgtcga gctggattca caaggccgca tcatcgctcc ggaaggcaac
    69901 gacaacgctt tccagaccag caacccgaaa atctttgctg gcggcgatat cgtccgtggt
    69961 tccgatctgg tcgttaccgc catcgccgaa ggtcgcaaag cggcagacgg cattatgaac
    70021 tggctggaag tttaagctct gtcccagcgc cgcctcctca gaaaagagtc ggcgctgaaa
    70081 ttctcctttc tttctggaaa tcgcctcgca taatctgaag aaaaccaatg taatacgctc
    70141 atctggattg ttaccggtag atgcccggcg tatagctgat tccattattc aacgcagcgc
    70201 catataaagg gacagataat ggaatcgctc tcagagagaa cctcaacagg ctaccagcaa
    70261 atccacaacg gcattattca tctggtcgat agcgcccgaa cggaaacggt acgtagcgtt
    70321 aacgcgctga tgaccgcgac gtactgggaa attggccgac ggattgtcga atttgaacaa
    70381 ggtggtgagg ccagagcagc ttatggtgca cagttgattg atcggctatc gaaggattta
    70441 agtcgacggt ataaacgtgg ggtctctgca aaaaacttac gccaaatgag gcttttttac
    70501 ctcttttttc aacatgttga aattcgccag acagtgtctg gcgaattaac accattggga
    70561 attccgcaga caccatctgc agaattccca tcggcaaaga tttggcagac gctgtctgcc
    70621 aaattgagag gctgtttgat atttccacag cttttgtatt gcatctccta ttttcggttt
    70681 gtatattcat gcaaataaac gaag
//
LOCUS       DAEEUE010000007        67100 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.007, whole genome shotgun sequence.
ACCESSION   DAEEUE010000007 DAEEUE010000000
VERSION     DAEEUE010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67100
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.007"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(64..1068)
                     /gene="cra"
                     /locus_tag="KLK84_000493"
     CDS             complement(64..1068)
                     /gene="cra"
                     /locus_tag="KLK84_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HBE2007954.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1150..1236)
                     /locus_tag="KLK84_000494"
     CDS             complement(1150..1236)
                     /locus_tag="KLK84_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HBE2007955.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1248..1739)
                     /gene="ilvN"
                     /locus_tag="KLK84_000495"
     CDS             complement(1248..1739)
                     /gene="ilvN"
                     /locus_tag="KLK84_000495"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBE2007956.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1742..3466)
                     /gene="ilvI"
                     /locus_tag="KLK84_000496"
     CDS             complement(1742..3466)
                     /gene="ilvI"
                     /locus_tag="KLK84_000496"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HBE2007957.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3585..3629
                     /locus_tag="KLK84_000497"
     CDS             3585..3629
                     /locus_tag="KLK84_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2007958.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3784..4728)
                     /gene="leuO"
                     /locus_tag="KLK84_000498"
     CDS             complement(3784..4728)
                     /gene="leuO"
                     /locus_tag="KLK84_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HBE2007959.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5388..5474
                     /gene="leuL"
                     /locus_tag="KLK84_000499"
     CDS             5388..5474
                     /gene="leuL"
                     /locus_tag="KLK84_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HBE2007960.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5567..7138
                     /gene="leuA"
                     /locus_tag="KLK84_000500"
     CDS             5567..7138
                     /gene="leuA"
                     /locus_tag="KLK84_000500"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBE2007961.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7138..8229
                     /gene="leuB"
                     /locus_tag="KLK84_000501"
     CDS             7138..8229
                     /gene="leuB"
                     /locus_tag="KLK84_000501"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBE2007962.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8232..9632
                     /gene="leuC"
                     /locus_tag="KLK84_000502"
     CDS             8232..9632
                     /gene="leuC"
                     /locus_tag="KLK84_000502"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBE2007963.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9643..10248
                     /gene="leuD"
                     /locus_tag="KLK84_000503"
     CDS             9643..10248
                     /gene="leuD"
                     /locus_tag="KLK84_000503"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBE2007964.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10297..11475)
                     /gene="setA"
                     /locus_tag="KLK84_000504"
     CDS             complement(10297..11475)
                     /gene="setA"
                     /locus_tag="KLK84_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HBE2007965.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="KLK84_000505"
     CDS             complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="KLK84_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HBE2007966.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11797..13452
                     /gene="sgrR"
                     /locus_tag="KLK84_000506"
     CDS             11797..13452
                     /gene="sgrR"
                     /locus_tag="KLK84_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HBE2007967.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13486..13585
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13616..14599
                     /gene="thiB"
                     /locus_tag="KLK84_000507"
     CDS             13616..14599
                     /gene="thiB"
                     /locus_tag="KLK84_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HBE2007968.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14575..16185
                     /gene="thiP"
                     /locus_tag="KLK84_000508"
     CDS             14575..16185
                     /gene="thiP"
                     /locus_tag="KLK84_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HBE2007969.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16169..16867
                     /gene="thiQ"
                     /locus_tag="KLK84_000509"
     CDS             16169..16867
                     /gene="thiQ"
                     /locus_tag="KLK84_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HBE2007970.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16981..17745)
                     /locus_tag="KLK84_000510"
     CDS             complement(16981..17745)
                     /locus_tag="KLK84_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBE2007971.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17831..18709)
                     /gene="araC"
                     /locus_tag="KLK84_000511"
     CDS             complement(17831..18709)
                     /gene="araC"
                     /locus_tag="KLK84_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HBE2007972.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQR
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19048..20748
                     /gene="araB"
                     /locus_tag="KLK84_000512"
     CDS             19048..20748
                     /gene="araB"
                     /locus_tag="KLK84_000512"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HBE2007973.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20759..22261
                     /gene="araA"
                     /locus_tag="KLK84_000513"
     CDS             20759..22261
                     /gene="araA"
                     /locus_tag="KLK84_000513"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBE2007974.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22461..23156
                     /gene="araD"
                     /locus_tag="KLK84_000514"
     CDS             22461..23156
                     /gene="araD"
                     /locus_tag="KLK84_000514"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBE2007975.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23231..25582
                     /gene="polB"
                     /locus_tag="KLK84_000515"
     CDS             23231..25582
                     /gene="polB"
                     /locus_tag="KLK84_000515"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HBE2007976.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25747..28653
                     /gene="rapA"
                     /locus_tag="KLK84_000516"
     CDS             25747..28653
                     /gene="rapA"
                     /locus_tag="KLK84_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HBE2007977.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSSEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQELAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHTILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28665..29324
                     /gene="rluA"
                     /locus_tag="KLK84_000517"
     CDS             28665..29324
                     /gene="rluA"
                     /locus_tag="KLK84_000517"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HBE2007978.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="KLK84_000518"
     CDS             complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="KLK84_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HBE2007979.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30511..32865
                     /gene="lptD"
                     /locus_tag="KLK84_000519"
     CDS             30511..32865
                     /gene="lptD"
                     /locus_tag="KLK84_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HBE2007980.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32918..34204
                     /gene="surA"
                     /locus_tag="KLK84_000520"
     CDS             32918..34204
                     /gene="surA"
                     /locus_tag="KLK84_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HBE2007981.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34204..35193
                     /gene="pdxA"
                     /locus_tag="KLK84_000521"
     CDS             34204..35193
                     /gene="pdxA"
                     /locus_tag="KLK84_000521"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HBE2007982.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35190..36011
                     /gene="rsmA"
                     /locus_tag="KLK84_000522"
     CDS             35190..36011
                     /gene="rsmA"
                     /locus_tag="KLK84_000522"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBE2007983.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36014..36391
                     /gene="apaG"
                     /locus_tag="KLK84_000523"
     CDS             36014..36391
                     /gene="apaG"
                     /locus_tag="KLK84_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HBE2007984.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGTIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36398..37240
                     /gene="apaH"
                     /locus_tag="KLK84_000524"
     CDS             36398..37240
                     /gene="apaH"
                     /locus_tag="KLK84_000524"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HBE2007985.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37318..37797)
                     /gene="folA"
                     /locus_tag="KLK84_000525"
     CDS             complement(37318..37797)
                     /gene="folA"
                     /locus_tag="KLK84_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HBE2007986.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="KLK84_000526"
     CDS             complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="KLK84_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HBE2007987.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="KLK84_000527"
     CDS             complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="KLK84_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HBE2007988.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40421..41815)
                     /locus_tag="KLK84_000528"
     CDS             complement(40421..41815)
                     /locus_tag="KLK84_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE2007989.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="KLK84_000529"
     CDS             complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="KLK84_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HBE2007990.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42158..43444)
                     /locus_tag="KLK84_000530"
     CDS             complement(42158..43444)
                     /locus_tag="KLK84_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBE2007991.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43495..44436)
                     /locus_tag="KLK84_000531"
                     /pseudo
     CDS             complement(43495..44436)
                     /locus_tag="KLK84_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44451..45221)
                     /locus_tag="KLK84_000532"
     CDS             complement(44451..45221)
                     /locus_tag="KLK84_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HBE2007992.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45693..47207
                     /gene="caiT"
                     /locus_tag="KLK84_000533"
     CDS             45693..47207
                     /gene="caiT"
                     /locus_tag="KLK84_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HBE2007993.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47238..48380
                     /gene="caiA"
                     /locus_tag="KLK84_000534"
     CDS             47238..48380
                     /gene="caiA"
                     /locus_tag="KLK84_000534"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HBE2007994.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48509..49726
                     /gene="caiB"
                     /locus_tag="KLK84_000535"
     CDS             48509..49726
                     /gene="caiB"
                     /locus_tag="KLK84_000535"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HBE2007995.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49800..51353
                     /gene="caiC"
                     /locus_tag="KLK84_000536"
     CDS             49800..51353
                     /gene="caiC"
                     /locus_tag="KLK84_000536"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HBE2007996.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51462..52247
                     /gene="caiD"
                     /locus_tag="KLK84_000537"
     CDS             51462..52247
                     /gene="caiD"
                     /locus_tag="KLK84_000537"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HBE2007997.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52253..52843
                     /gene="caiE"
                     /locus_tag="KLK84_000538"
     CDS             52253..52843
                     /gene="caiE"
                     /locus_tag="KLK84_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HBE2007998.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52929..53324)
                     /gene="caiF"
                     /locus_tag="KLK84_000539"
     CDS             complement(52929..53324)
                     /gene="caiF"
                     /locus_tag="KLK84_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HBE2007999.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(53586..56807)
                     /gene="carB"
                     /locus_tag="KLK84_000540"
     CDS             complement(53586..56807)
                     /gene="carB"
                     /locus_tag="KLK84_000540"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBE2008000.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56825..57973)
                     /gene="carA"
                     /locus_tag="KLK84_000541"
     CDS             complement(56825..57973)
                     /gene="carA"
                     /locus_tag="KLK84_000541"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBE2008001.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58429..59250)
                     /gene="dapB"
                     /locus_tag="KLK84_000542"
     CDS             complement(58429..59250)
                     /gene="dapB"
                     /locus_tag="KLK84_000542"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBE2008002.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59417..60331)
                     /gene="rihC"
                     /locus_tag="KLK84_000543"
     CDS             complement(59417..60331)
                     /gene="rihC"
                     /locus_tag="KLK84_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HBE2008003.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60397..61347)
                     /gene="ispH"
                     /locus_tag="KLK84_000544"
                     /gene_synonym="lytB"
     CDS             complement(60397..61347)
                     /gene="ispH"
                     /locus_tag="KLK84_000544"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBE2008004.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61349..61798)
                     /gene="fkpB"
                     /locus_tag="KLK84_000545"
     CDS             complement(61349..61798)
                     /gene="fkpB"
                     /locus_tag="KLK84_000545"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBE2008005.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61885..62379)
                     /gene="lspA"
                     /locus_tag="KLK84_000546"
     CDS             complement(61885..62379)
                     /gene="lspA"
                     /locus_tag="KLK84_000546"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBE2008006.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62379..65195)
                     /gene="ileS"
                     /locus_tag="KLK84_000547"
     CDS             complement(62379..65195)
                     /gene="ileS"
                     /locus_tag="KLK84_000547"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBE2008007.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(65238..66179)
                     /gene="ribF"
                     /locus_tag="KLK84_000548"
     CDS             complement(65238..66179)
                     /gene="ribF"
                     /locus_tag="KLK84_000548"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBE2008008.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(66187..66405)
                     /locus_tag="KLK84_000549"
     CDS             complement(66187..66405)
                     /locus_tag="KLK84_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HBE2008009.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            66508..66771
                     /gene="rpsT"
                     /locus_tag="KLK84_000550"
     CDS             66508..66771
                     /gene="rpsT"
                     /locus_tag="KLK84_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBE2008010.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        16290 a        18259 c        17267 g        15284 t
ORIGIN      
        1 tgtccagtcc cgtactctac gcgccagagg gaaattcacc tggcgcgtat ttttgttcgc
       61 ggattagcta cggctgagca cgccgcggcg atagagatta cgtttaatgc gcgttaaacc
      121 aggttttggc ttacgcggtt cgtccaggct tgccaggaca atctccagca cacgctctgc
      181 gacatcgcgg tgacgttgag ccactgccag caccggacac tgtaagaagt cgagcagttc
      241 gttatcgcca aaggtggcaa ttgccaggtc agaaggcagt ttgccgtcgc gacgcagcgt
      301 gacatccatc actccttgca acaacgcaaa cgacgttgtg aacagcgcct gcggcatcgg
      361 atgcgtttcc agccattttt cgaataactg ggcagccgcc tcccgctcat agctgttggc
      421 atacaggaaa tgcacttcgc gcggatcatc tttccaagca gtacggaaac cttgttcacg
      481 caggaagctg acagaaagct ccggcagcgc gccaagataa agcaccgtct cggcgggaaa
      541 cttacgtaac tcttccgcca gcatttcggc atcatcctga tcggcaccaa ccacgctggt
      601 gaaatgttca cgatcgaggg cgcggtccag cgcgacaatc gggaacgggt cgttagccca
      661 gcgttgataa aaaggatgct caggtggcaa cgacgtcgaa acaataatgg catcaacctg
      721 acgctgtaaa aggtgctcaa tgcagcgcat ttcgttgtct ggctgatctt ctgagcaggc
      781 aatcagcagt tgataacccc gttgccgcgc ctggcgttca agatagttag cgatgcgggt
      841 atagctggtg ttctccagat cggggatcac aagaccaata gaacgtgtgc gtccagcacg
      901 aagcccagct gccacggcgt tcgggtggta attgtgctca cgcaccacag ccatgacttt
      961 ttcaacggtt ttgtcgctca cacggtattg cttcgctttg ccgttaataa catagcttgc
     1021 agtggtccgc gacactcccg ccagccgagc gatttcatcc agtttcacaa ttgccccttg
     1081 cgtaaaatgt aaaaaccata gccattgtac tggtatgggt taaattcatt aacatctaag
     1141 cgcagaaaat taactgcggc aactgctttt attccaggtt cccactgatt tcgcaaaaaa
     1201 agcccaacgt gacatgttgg gctgcaaatt gcgcactgag atcattctca acgcattatt
     1261 ttatcgccgc gcgaaagtcc gaccacacca gagcgagcaa cttccacaat tttcgccaca
     1321 tcgcgaatcg atgctaaaaa tgcatcaagc ttatcgctgg tgcctgctaa ttgaacggta
     1381 taaagcgagg gggtgacatc gataatttgc ccacggaata tttccgtatt acgtttcact
     1441 tcgtcacgcc cgtaaccgct ggcctgaatt ttcaccagca tgatttcccg ctcaacatgc
     1501 gcgccctgcc ccaactcact cacgcgcaag acatcgacca gcttgtgtaa ttgcttttcg
     1561 atctgctcaa gtactttttc atcgcccacg gtctggatgg tcatacgcga taatgtcgga
     1621 tcgtcggttg gcgcaacggt caggctttca atgttgtagc ctcgctggga aaaaaggcca
     1681 atcacgcggg ataacgcgcc tgattcattt tcgagtaaga ctgataatat ccggcgcata
     1741 atcaggttct ctccgttttg cttaaccaca tttcatccat tccgcccccg cgaatctgca
     1801 tcgggtagac gtgctcactg ccatcgacgg taacatcaac aaacaccagg cgattattgc
     1861 gcacctgttc cagcgcctcg ctaagtttgc tttccagctc atgcggatga gagatctgga
     1921 tcccgacgtg accataggct tccgccagac ggacgaaatc gggtagtgat tgcatataag
     1981 attgtgaatg acggccggaa tagatcatgt cctgccactg tttcaccatc cccagatagc
     2041 ggttattgag attcaccacc agtacgggca actcgtattg caacgcggta gacagttcct
     2101 ggatgttcat ctgaatactg ccgtcgccag tgacgcaaac cacggtttct tctggcaacg
     2161 ccattttgac gcccagtgcc gcaggtaaac caaaacccat cgtgccgaga ccgccggaat
     2221 tgatccagcg acgcggtttg tcgaatggat aataaagcgc agcaaacatc tggtgctgcc
     2281 cgacatcgga cgtcacgtaa gcgtccccat tagtcaaccg ccaaagagtc tcgatcaccg
     2341 cctgcggttt aatcttttca ctgtgagtgt catatttcag gcactgaaga gcgcgccact
     2401 gttcaatctg ctgccaccag tcgcggatct catccagcgg ttgatgggcg gattcttgcg
     2461 acaggagttc aagcatttgt tcgaggacct ggcgagcatc ccccacaatc gggatatccg
     2521 cagtcacggt tttagaaatg gaagtaggat caatatcgat atgcagaaca gtggcatttg
     2581 ggcagtactt tgccagattg ttcgtcgttc ggtcatcaaa tcgtaccccg acggcgaaaa
     2641 tcacatccgc gttatgcatc gtcatattgg cttcgtaggt accgtgcatt cccagcatgc
     2701 ccagtgcctg acgatgcgtt gccggaaacg cccccagccc catcaatgag gaaacaacgg
     2761 gcagattcaa cgcctccacc gtttctttca actgctgatg gcagcccgcc atgattgccc
     2821 caccgcctac gtagacaacc ggtttttttg ccgctaccag cgtttgcaga gcacgcttaa
     2881 tttgcccttt atgtccggta gtagtgggat tgtaagaacg catactgacc gactcagggc
     2941 agacataggg taatttgttc gccggattaa gaatatcttt cggtaaatca acgaccactg
     3001 gtcctgggcg accacttgcc gccagccaga aagccttttt cagcacctgc ggaatgtctt
     3061 ccgtttgctt aaccagaaaa ctgtgtttaa ccaccggtcg cgaaatcccc accatgtcgc
     3121 actcctgaaa ggcatcgtaa cctatcaacg aggtcgctac ctgcccggaa aggacaacta
     3181 atggaatgga atccatataa gcggtggcga tgccggtaat cgcattggtc gcccctggac
     3241 ccgacgttac cagcacgacg ccgacttccc cggtcgcacg cgccaggcca tcggccatat
     3301 gcaccgccgc ctgctcatga cgaactaata catgatcaat accacccacg gtatgcaatg
     3361 catcgtaaat atcaaggact gcgcctccgg gataaccgaa tacttgttta acgccctgat
     3421 cgataagcga tcggacgacc atctcggctc cagacaacat ctccatggcc tgcctcactg
     3481 tttgacggaa aaaatgtgta aaacacatta accgtccgat cttgcttaag caattggcag
     3541 ccagagacat aaacgcaggg taataagaat aaacaggagg aaaggtgaaa agaaacgata
     3601 aaatcctcca ttacgaagga ttaaattgat aataaatcgg ctgaatccca caacttacag
     3661 caatttctct atttttttaa ttaatcctaa aatagagagt ccacacattc agaataaaat
     3721 tctacattca ataaaaaatg cagaataaac cagacattca tgtctggcct attctgcatt
     3781 cagttagcgt ttgcaaattg agactaattg ttcttccatc cactgatggc ctttatcgcg
     3841 cccggcagct tcatgccaag agagataaca ggttctgctg ttttgtttta acggcagcgg
     3901 taatacctgt aattctaagg attcagcgaa ctcttcagcc agccaacgcg gcgcaatagc
     3961 gaccaaatgc gtttgcgaca ccacgctaag tacgctcatc attgccatgc cctgatacgc
     4021 gatactggct tgcttatcta ccgtgtcata ccaaggttga ctaaatgacg cgaaacgatc
     4081 gagcgaaacc gccgcatgtt gttcgttata aacatcatgt ttcagtaacg ggcctttaat
     4141 tgttggatga tttttgctgg ctaccagcac catttcatct ttaaataatg gcacgctggt
     4201 aaattcagga cgatggaagt cttcataact aatcacaaac tccgtttcct gataacgcag
     4261 ctgatgttca gtgttctgat ttaatgaaga cttgaacata acatgtatat ttggcgcaat
     4321 ctgctcaatg tgattataaa tctgcgaggt cagaatgctg tctaacgggc tgcaaacaca
     4381 aagatgaaat acacgttcac tgctcgcggg ttcaaaacct gaaccaggca attcattttg
     4441 tactagttgc aatgcctggc gaactgaacc aaaaagttga aatgcgcgag cagtcggttg
     4501 aataccacgg ccataacgaa caaaaagctc gtcattaaac atcaccttca ggcgtgcaac
     4561 agcgttactg accgcaggtt gcgacatacc cagaacatga gcggcacggg taatattttg
     4621 ctcctgcatc acggcatcga aaacggttaa taagttgaga tcgaccatgc gtagctgtgg
     4681 cttgcttaac tccgccgtct ctgaatgatc tgtttgtacc tctggcatac ttaactccac
     4741 tgtcacactt aactcccttt cccttattgg aatgcagaga aataatcctg aagaatatga
     4801 tttatcatgc atataaaata agaaaaagca aaatgagtaa aattcggaaa acataaagat
     4861 acgggttctg ataaaaacag aatccattgc cgtggaatga gtcatttacc acagcataat
     4921 aatccataat gtttttgttg cgaaaacaat ctaatcataa ctaccgcgaa tactcaatca
     4981 tctacaaaat ggattaaatg tgcttattta ataattaatt tatatattta atgcattaat
     5041 tcttaacatt aattgatcaa taatattcac caaatcaata tcaaaaaaaa tcgcaaaaca
     5101 tataattcaa tacaaatcat caggataggt tttgcaacgc gtgcattttg tccccttttt
     5161 cctcgttgat tagatgcaaa aatttatgct gaaatatgtc aaccgatgaa aagcgtcggt
     5221 agttaagcag aaattaatat cgcttacttt aaccaccgta gcacaattag ctaattttac
     5281 ggatgcagaa ctcacgctgg cgggacgttt ttattgcgtc agggttgaca tccgtttttg
     5341 tatccagtaa ctctaaaagc atatcgcatt catctggagc tgatttaatg actcacatcg
     5401 ttcgctttat cggtctacta ctactaaacg catcttcttt gcgcggtaga cgagtgagcg
     5461 gcatccagca ttaagccagc acgcagtcaa acaaaaaacc cgcgccattg cgcgggtttt
     5521 tttatgcccg aagcgaggcg ctctaaaaga gacaaggacc caaaccatga gccagcaagt
     5581 cattattttc gataccacat tgcgcgacgg tgaacaggcg ttacaggcaa gcttgagtgt
     5641 gaaagaaaaa ctgcaaattg cgctggccct tgagcgtatg ggtgttgacg tgatggaagt
     5701 cggtttcccc gtctcttcgc cgggcgattt tgaatcggtg caaaccatcg cccgccaggt
     5761 taaaaacagc cgcgtatgtg cgttagctcg ctgcgtggaa aaagatatcg acgtggcggc
     5821 cgaatccctg aaagtcgccg aagccttccg tattcatacc tttattgcta cctccccaat
     5881 gcacatcgcc accaagctgc gcagcaccct agacgaggtg atcgaacgcg ctatctatat
     5941 ggtgaaacgc gcccgtaatt acaccgatga tgttgaattt tcttgcgaag atgccgggcg
     6001 tacacccatt gccgatctgg cgcgagtggt cgaagcggcg attaatgccg gtgccaccac
     6061 catcaacatt ccggacaccg tgggctacac catgccgttt gagttcgccg gaatcatcag
     6121 cggcctgtat gaacgcgtac ctaacatcga caaagccatt atttccgtac atacccacga
     6181 cgatttaggc ctggcggtcg gcaactcact ggcggcggta catgccggtg cacgccaggt
     6241 ggaaggtgca atgaacggga tcggcgagcg tgccggtaac tgttcgctgg aagaagtcat
     6301 catggcgatt aaagttcgta aggatattct caatgtccac accgccatta atcaccaaga
     6361 gatatggcgc accagccagt tagttagcca gatttgtaat atgccgatcc cggcaaacaa
     6421 agccattgtt ggcagcggcg cattcgctca ctcctccggt atccaccagg atggcgtgct
     6481 gaaaaaccgc gaaaactacg aaatcatgac accagaatct attggtctga accaaatcca
     6541 gctgaatctg acctctcgtt cggggcgtgc ggcggtgaaa catcgcatgg atgagatggg
     6601 gtataaagaa agtgaatata atttagacaa tttgtacgat gctttcctga agctggcgga
     6661 caaaaaaggt caggtgtttg attacgatct ggaggcgctg gccttcatcg gtaagcagca
     6721 agaagagccg gagcatttcc gtctggatta cttcagcgtg cagtctggct ctaacgatat
     6781 cgccaccgcc gccgtcaaac tggcctgtgg cgaagaagtc aaagcagaag ccgccaacgg
     6841 taacggtccg gtcgatgccg tctatcaggc aattaaccgc atcactgact ataacgtcga
     6901 actggtgaaa tacagcctga ccgccaaagg tcacggtaaa gatgcgctgg gtcaggtgga
     6961 tattgtcacc aactacaacg gtcgccgctt ccacggcgtc ggcctggcca ccgatattgt
     7021 cgagtcctcc gccaaagcca tggtgcacgt actgaacaat atctggcgtg ccgcagaagt
     7081 cgaaaaagag ttgcaacgca aaactcaaca caacgaaaac aacaaggaaa ccgtgtgatg
     7141 tcgaagaatt accatattgc cgtattgccg ggagacggta ttggtccgga agtgatgacc
     7201 caggcgctga aagtgctgaa tgccgtgcgc aaccgctttg cgatgcgcat caccaccagc
     7261 cattacgatg taggcggcgc agccattgat aaccacgggc aaccactgcc gcctgcgacg
     7321 gttgaaggtt gtgagcaagc cgatgccgtg ctgtttggct cggtaggcgg cccgaagtgg
     7381 gaacatttac caccagacca gcaaccagaa cgcggcgcgc tgctgccgct gcgtaagcac
     7441 ttcaaattat tcagcaacct gcgcccggca aaactgtatc aggggctgga agcattctgt
     7501 ccgctgcgtg cagacattgc cgctaacggc ttcgacatcc agtgcgtgcg cgaactgacc
     7561 ggcggcatct atttcggtca gccaaaaggc cgcgaaggta gcggacaata tgaaaaagcg
     7621 tttgataccg aggtgtatca ccgttttgag atcgagcgta ttgcccgcat cgcgtttgaa
     7681 tctgctcgta aacgtcgcca caaagtcacc tcaatcgaca aagctaacgt gctgcaatcc
     7741 tctattttat ggcgtgaaat cgtcaacgag atcgccacgg aatacccgga tgtcgaactg
     7801 gcgcatatgt acatcgacaa cgccaccatg cagctgatta aagatccatc acagtttgac
     7861 gttctgctgt gctccaacct gtttggcgac attctgtctg acgagtgcgc aatgatcact
     7921 ggctcgatgg gcatgttgcc ttccgccagc ctgaacgagc aaggttttgg actgtatgaa
     7981 ccggcgggcg gctcggcacc agatatcgca ggcaaaaaca tcgccaaccc gattgcacaa
     8041 atcctgtcgc tggcactgct gctgcgttac agcctggatg ccgatgatgc ggcttccgcc
     8101 attgaacgcg ccattaaccg tgcattagaa gaaggcattc gcaccgggga tttagcccgt
     8161 ggcgctgccg ccgttagtac cgatgaaatg ggcgatatca ttgcccgcta tgtggcagaa
     8221 ggggtgtaat catggctaaa acgttatacg aaaaattatt cgacgctcac gtagtgtacg
     8281 aagccgaaaa cgaaaccccg ctgttatata tcgaccgcca cctggtgcat gaagtgacct
     8341 caccgcaggc gttcgatggc ctgcgcgccc acggtcgccc ggtacgtcag ccgggcaaaa
     8401 ccttcgccac tatggatcac aacgtctcta cccagaccaa agacattaat gcctgtggtg
     8461 aaatggcgcg catccagatg caggagctga ttaaaaactg caaagaattt ggcgtcgagc
     8521 tgtatgacct gaatcacccg tatcagggga tcgtccacgt aatggggccg gaacagggcg
     8581 tcaccttgcc ggggatgacc attgtctgcg gcgactcgca taccgccacc cacggcgcgt
     8641 ttggcgcact ggcctttggt atcggcactt ccgaagttga acacgtactg gcaacacaaa
     8701 ccctgaaaca gggccgtgcg aagaccatga aaattgaagt ccagggcaaa gccgcgccgg
     8761 gcattaccgc aaaagatatc gtgctggcaa ttatcggtaa aactggcagc gcaggcggca
     8821 ccgggcatgt ggtggagttt tgcggcgaag caattcgtga tttaagcatg gaaggtcgta
     8881 tgaccctgtg caatatggcg atcgaaatgg gcgcaaaagc cggtctggtt gctcctgacg
     8941 aaaccacctt taactatgtc aaaggccgtc tgcatgcgcc gaaaggcaaa gatttcgacg
     9001 acgccgttgc ctactggaaa accctgcaaa ccgacgaagg cgcaactttc gataccgttg
     9061 tcactctgca agcagaagaa atttcaccgc aggtcacctg gggcaccaat cccggccagg
     9121 tgatttctgt gaacgacaat attcccgatc cggcttcgtt tgccgatccg gttgaacgtg
     9181 cgtcggcaga aaaagcgctg gcctatatgg ggctgaaacc gggtattccg ctgaccgaag
     9241 tggctatcga caaagtgttt atcggttcct gtaccaactc gcgcattgaa gatttacgcg
     9301 cggcagcgga gatcgccaaa gggcgaaaag tcgcgccagg cgtgcaggca ctggtggttc
     9361 ccggctctgg cccggtaaaa gcccaggcgg aagcggaagg tctggataaa atatttattg
     9421 aagccggttt tgaatggcgc ttgcctggct gctcaatgtg tctggcgatg aacaacgacc
     9481 gtctgaatcc gggcgaacgt tgtgcatcca ccagcaaccg taactttgaa ggccgccagg
     9541 ggcgcggcgg gcgcacgcat ctggtcagcc cggcaatggc tgccgctgct gctgtgaccg
     9601 gacatttcgc cgacattcgc aacattaaat aaggagcaca ccatggcaga gaaatttatc
     9661 aaacacacag gcctggtggt tccgctggat gccgccaatg tcgataccga tgcaatcatc
     9721 ccgaaacagt ttttgcagaa agtgacccgt acgggttttg gcgcgcatct gtttaacgac
     9781 tggcgttttc tcgatgaaaa aggccaacag ccaaacccgg acttcgtgct gaacttcccg
     9841 cagtatcagg gcgcttccat tttgctggca cgagaaaact tcggctgtgg ctcctcgcgt
     9901 gagcacgcgc cctgggcatt gaccgactac ggttttaaag tggtgattgc gccgagtttt
     9961 gctgacatct tctacggcaa tagctttaac aaccagctgc tgccggtgaa attaagcgat
    10021 gcagaagtgg acgaactgtt tgcgctggtg aaagccaatc cggggatcca tttcgacgtg
    10081 gatctggaag cgcaagaggt gaaagcggga gagaaaacct atcgctttac catcgatgcc
    10141 ttccgccgcc actgcatgat gaatggtctg gacagcatcg gcctgaccct gcagcacgac
    10201 gacgctattg cgtcttatga agcaaaacaa cctgcgttta tgaattagac cccttgcccg
    10261 gtcaaatgac cgggctttcc gctatcgtcc acgtcatcaa acgtctttaa cctttgcggt
    10321 taaaaataat gcgacaacag aaataatcgc aattacccag tagacagcaa agtgccccca
    10381 actttgtgca attgctccct gaataacacc agccagaatt accccggtag agatagtgtt
    10441 ggtaaataag gtggtagctg cccccgctct tccaggcatt aaatcctgaa accatagcat
    10501 cccaataccc gcaacaatgc cgataaatac agcgttaaaa agttgcagcg tcatcaacgc
    10561 catacggcta tgaaagaaaa tcaatccagt gtaaaacagc actcctgcca ccactgcaat
    10621 gaccatcatt cgccgcttac cataacgttt gacatagtag ccagccagaa tcattgctgg
    10681 tatttccagt ccagctgccg tccccatcag gaaacccgcc agtttgtctg gcaatcctaa
    10741 atcgctactg atccacaatg gcatatcaat aatgtacata gtgttgcagg tccacattaa
    10801 cgtcgaggcg acaaataaca tccgtacgtt actatcctgc cagtcacctt gcattgataa
    10861 agcattttcc gacggcagtt ctacccgcgc cacagacgga agcataaatg caatcaatac
    10921 cagactgagt gtgaatatcc cggcggcaat cgaaaacatc actgtaaagc cgtaattcaa
    10981 cgccagcata aaggccagcg gtggaccgat tacccatgcc agagaaagct gcgcacgcat
    11041 caccgagcta aacatcacca cttctcgcgc cgagttatcc gcgtattccc gcgccagagc
    11101 aaataactgt ggcattgccg tattggccag agatgccaga agcacaccac aggtgataag
    11161 cgtcagataa tgacgattaa atgcaaacag tagcgcattg ccaatagcca tcaaacagca
    11221 aaatataatc agttttcgcc gatcgccctg actgtcagaa cgttttgcca accagaggct
    11281 tacgccgatc ccagcaatag cattcaccgt ataaaagagt ccgatccaga aaggttgcgc
    11341 gccaacctca cgactcagaa ataagctcaa tgtaggagcc tgtagcgccc cggccacccc
    11401 catcataaaa gcgaccagca taaatgccgc gtaaacaccg ttcatacggc gagccatcgt
    11461 cattatccag atcatacgtt ccctttttag cgcggcgaga ataaaaaaaa ccagcaggta
    11521 taatctgctg gcgggtgatt ttacaccaat actcagtcac acatgatgca ggcaagtcaa
    11581 ctttcagaat tgcggtcatc ccactgcatc agtccttcaa gcatggttaa tcgttgtggg
    11641 acgcttaacc aacgcaacca gcacaacttc gctgtcgcgg taaaatagtg ctgataaaac
    11701 tgacgcatgg ggcacctcct tgcttcatcg ttaggaatta ttgacttaat atagggaaaa
    11761 taaaattgct gtcttttgcg caggagttcc ccttttatgc catctgctcg tctgcaacaa
    11821 cagttcatcc gcctgtggca atgctgcgag ggtaaatcgc aggacacaac gctcaacgaa
    11881 ctggcagcgt tattgagctg ctcgcgtcgt catatgcgta ccctgctcaa caccatgcag
    11941 gatcgcggct ggctgacgtg ggaagcggaa gtcgggcgcg gtaaacgctc gcgtctgaca
    12001 ttcctctata ccgggctggc gcttcagcaa cagcgggcgg aagacctgct ggagcaggat
    12061 cgtatcgacc aactagtgca gttggttggc gacaaagcga ctgtgcggca aatgctggtt
    12121 tctcatctgg gccgcagctt ccgccagggg cggcacatcc tgcgcgtgct ctactatcgt
    12181 ccgttgcgta atctgctacc tggcagcgca ttgcgccgtt ccgaaaccca tattgcccgg
    12241 caaatcttca gttcgctaac gcgcataaat gaggaaaatg gggaactgga agcagacatc
    12301 gcccaccact ggcagcaaat atcaccgctt cactggcgtt tctttttgcg tccaggagtc
    12361 cattttcatc atggtcgtga actggaaatg gacgacgtga tcgcctcttt aaaacgaatc
    12421 aatacgctgc cgctctattc gcatattacg gacattgtgt cgccgacgcc ctggacgctg
    12481 gatatccatc tcacgcagcc ggaccgctgg ttgccgttac tgctggggca agttccggcg
    12541 atgatcctgc cgtgcgaatg ggaaaccctc agtaactttg ccagccatcc tattggcacc
    12601 ggtccgtatg cggtgattcg caacagcacc aatcaactga aaattcaggc attcgatgac
    12661 ttcttcggtt accgggcatt aatcgacgaa gttaacgtct gggttctgcc ggaaattgcc
    12721 gacgagccag ccggtggtct gatgctaaaa ggtccacagg gcgagaaaaa agagattgaa
    12781 agccgcctgg aggaaggttg ctactattta ctgttcgaca gccgcaccca tcgcggggcg
    12841 aatcagcaag tcagggactg ggtaagctat gtgctttctc caactaatct ggtctatttc
    12901 gctgaggaac agtaccagca actgtggttc ccggcttatg gactgctccc ccgttggcac
    12961 catgcccgca ccataaagag cgaaaaaccg gctggcctgg aaagcctcac cctgaccttt
    13021 tatcaggatc acagtgagca tcgggtgatt gccgggatca tgcagcagat tctggcaagt
    13081 caccaggtca cgctggaaat caaagagatc agctacgatc agtggcatga aggagagatc
    13141 gagagcgata tctggcttaa tagcgccaac tttacgctgc cgctggattt ttcgctgttc
    13201 gcgcacttgt gcgaggtgcc gctgctccaa cactgtattc cgatcgactg gcaagccgac
    13261 gccgcccgct ggcgcaatgg cgaaatgaac ctggcgaact ggtgccagca actggtcgcc
    13321 agtaaagcaa tggtgccgct tatccaccac tggctgatca ttcagggaca acgcagtatg
    13381 cgcggcctgc gtatgaatac cctcggctgg ttcgatttta aatcagcgtg gtttgcgcca
    13441 ccggatccat gattgctggt agataacaaa atcactacac taacgccgtt ctcaacgggg
    13501 tgccacgcaa acgcgtgcgc tgagaaaata cccgtcgaac ctgatccgga taacgccggc
    13561 gaagggattt gaggctcctt ctcaagtcct ttgccactct tttttgaggt gcaaagtgtt
    13621 aaaaaaatgt cttcccctgc tgttgctgtg cacagcgccc gttttcgcta aacccgttct
    13681 gactgtttat acctacgatt ccttcgccgc cgactggggg cctggtccaa agatcaaaaa
    13741 agcttttgaa gccgactgta actgcgaact gaaactggtg gcgctggaag atggcgtttc
    13801 gcttctcaac cgtctacgga tggaaggcaa aaatagtaaa gccgatgtgg tgctggggct
    13861 ggataacaat ctgttagacg ccgccagtaa aaccggacta tttgccaaaa gcggtgtggc
    13921 agcggatgcc gttaacgttc ccggcggctg gaataatgac actttcgtcc cgtttgatta
    13981 cggctatttc gccttcgtct atgacaagaa caaactgaaa aacccgccgc aaagcctgaa
    14041 agaattggtt gagagcgatc agaactggcg ggtgatttat caggatccgc gtaccagcac
    14101 gccggggctg ggtctgctgt tgtggatgca aaaagtctat ggcgatgacg ccccacaagc
    14161 ctggcaaaaa ctggcgaaga aaaccgtcac ggtcaccaaa ggctggagcg aagcctacgg
    14221 cctgttttta aaaggtgaaa gcgatctggt actgagttac accacctctc cggcttatca
    14281 cattctcgaa gagaaaaaag ataactacgc cgccgcgaac tttagcgaag gtcactatct
    14341 gcaggtggaa gtcgccgccc gcaccgctgc cagcaagcag ccggagctgg cgcaaaaatt
    14401 cctccagttt atggtttctc cggcttttca gaatgcgatc ccaaccggta actggatgta
    14461 tccggtggca aatgtcacgc tgcctgccgg gtttgaacaa ttgaccaaac cggccaccac
    14521 gctggagttc acaccagctg aagtggcggc acaacgtcag gcatggatta gcgaatggca
    14581 acgcgccgtc agccgttaat tcccggctgg ttaattccag gtgtaagcgc cgccacgctg
    14641 gtggtagcgg ttgcgctcgc ggcgtttctc gccctgtggt ggaacgcgcc gcagggtaac
    14701 tgggtggcag tctggcagga cagctacctg tggcatgtgg tgcgcttctc cttctggcag
    14761 gcgtttctct cggcgctact ctctgtcgta cccgcgatat tcctcgcccg cgccctctat
    14821 cgcaggcgct ttccgggtcg gctggcgctg ttgcgtctgt gcgcaatgac cttgatcctc
    14881 ccggtgctag ttgctgtttt cggcattctt agcgtctatg gtcgtcaggg ctggctggca
    14941 tcgctctgcc aatcgctcgg tctggagtgg accttttcgc cctacggcct gcaaggcatt
    15001 ttgctggcgc acgtattttt taatctgccg atggcgagcc gcttattact ccaggcactg
    15061 gaaaacattc ccggcgaaca acgtcagctt gccgcccagc ttgggatgcg cggctggcat
    15121 tttttccgct tcgtcgaatg gccgtggcta cggcgacaaa tcccgccggt tgccgcgctt
    15181 atctttatgc tctgtttcgc cagcttcgcc accgtgctat cgctgggcgg cggtccgcag
    15241 gcaaccacta tcgagctggc tatttatcag gcgctgagtt acgactacga tcctgcccgc
    15301 gcggcgatgc tggcgctgct ccagatggtg tgttgccttg ggctggtgct gttgagtcag
    15361 cgactaagta aggccattgc gcccggcacc acgctgctgc aaggctggcg cgatccggac
    15421 gatcgtctgc atagccgcat ttgcgacacg gtgttaatag tgctggcgct gctgctgttg
    15481 ctgccaccgt tgctggcggt gatcgtcgat ggggtaaatc gccagttgcc ggaagtgctg
    15541 gcacaaccgg tgctgtggca ggcgctgtgg acctcgttgc gtattgcgct ggcggcaggt
    15601 gtattgtgcg tagtgctgac catgatgctg ctatggagca gtcgcgaact gcgggcgcgg
    15661 cagaaaatgc tggcgggtca ggcgctggag atgagcggca tgttgatcct cgccatgccg
    15721 gggattgtgc tggctaccgg cttcttttta ctgctcaaca acactatcgg cctgccacaa
    15781 tctgctgacg gcattgtgat tttcaccaat gcgttaatgg cgatccctta tgcgctgaaa
    15841 gtactggaaa acccgatgcg cgatatcacc gcccgctaca gtatgttgtg tcagtcgctg
    15901 ggcattgaag gctggtcgcg cttaaaagtg gtcgagctgc gcgccctgaa acgtccactg
    15961 gcgcaggcgc tggcttttgc ctgcgtgctg tcgattggtg attttggcgt agtggcgttg
    16021 ttcggtaacg atgatttccg taccctgccg ttttatctct accagcaaat tggctcctat
    16081 cgcagccagg acggcgcggt caccgcgtta attctgctac tactctgttt tctgctgttt
    16141 accgtgattg aaaaactacc ggggcgaaat gttaaaactg actgatatca cctggcttta
    16201 tcaccatttg ccgatgcgtt ttagcttaac ggtagaacgc ggcgagcagg tggcaatcct
    16261 cgggccaagc ggcgcgggta aaagtaccct gctgaatttg attgccggtt ttctgacgcc
    16321 agccagcggt tcgctgacta tcgatggtgt tgatcacacc acgacgccgc cgtcacgccg
    16381 tccggtgtcg atgctgtttc aggagaacaa cctgttcagc catctgacgg tcgcgcagaa
    16441 catcgggctg gggctaaatc cggggttgaa actgaacgcg gcacagcagg agaaaatgca
    16501 cgcgattgcc cgtcagatgg ggattgataa tttaatggcg cggttaccgg gcgagctttc
    16561 cggcggtcag cgacagcgcg tggcgttagc gcgttgtctg gtgcgcgaac agccgatttt
    16621 attgctcgat gaaccgttct ctgcgctcga tccggcgtta cgtcaggaga tgctgacgct
    16681 ggtgagcacg agctgccagc agcaaaaaat gacgctattg atggtgtcgc acagtgtgga
    16741 agatgcggcg cggatcgcca cacgctcggt ggtggtcgcc gacgggcgca tcgcctggca
    16801 gggtaagact gaagaattgt tgagcggtaa ggcgagtgct tcggcaatat tggggattac
    16861 gggttagtat gcactgtcag atgcggcgtg aacaccttat ccgacctacg gacagtgcgt
    16921 tttgtaggcc tgataagacg cggtcagcgt cgcatgaggc aacccgcaca agacaatccc
    16981 ctaaacccca accactttac gcaaaatatc gatatacacc ggcatcaacg ggtggcgaat
    17041 taacaccacc agcgccacca cgccgatggc agaaatcaac ggcgtcagcc acaacaaacg
    17101 gccgcgcgac aaataatgac ttaaacgatc ggttgcttta ccgctgcgcc ataaccgcca
    17161 gcacagccag ccaccaaccc acaaaaatac cgccgtcgcc agcagcaacc atttaaactc
    17221 gccgctctgc attccggctg gaatatcgat cgccgcgccc gccagaatcc caggcaggaa
    17281 gtaaaacggt ggccacagca ggcagccgat aatattcggc gtaatgaatt ttgccaccgg
    17341 cagatccagc attcccgcca ccattggcac cagcggacgc gtcgggccaa caaagcgacc
    17401 gaccagaatg gtgaacatgc tgtgttgatg caacgcatgt tcagttttat cgagtaatgc
    17461 tttgtttttc ttcagaaatg accagcgatg caacggcttt ttaaaacgcc agcccagcca
    17521 gaaggagatc cagtcgccca gcagacaacc gacaatccct gccagccaag cgtgccagaa
    17581 acttaactcg ccgctgccaa tcagcgctcc cagccccgcc atcagcaccg taccgggtag
    17641 aatcaaaccg accagcgcca gcgactccag aaaggccacc aacaccaccg ccatcaatga
    17701 atacacggta gattgggtaa taaagtgttc cagcaatgct tgcataatgt gcctgtcaaa
    17761 tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca attgtctgat
    17821 tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
    17881 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
    17941 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
    18001 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaaccgct
    18061 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
    18121 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacacgat
    18181 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
    18241 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
    18301 tttgcccaaa caggtcgctg aaatacggct ggtgcgcttc atccgggcga aagaaacccg
    18361 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
    18421 aaacccactg gtgataccat tcgcgagcct ccggatgaca accgtagtga tgaatctctc
    18481 ctggcgggaa cagcaaaata tcacccggtc ggcagacaaa ttctcgtccc tgatttttca
    18541 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
    18601 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
    18661 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
    18721 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
    18781 tcttttactg gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt
    18841 aacaaagcag gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
    18901 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
    18961 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
    19021 acccgttttt ttggatggag tgaaacgatg gcgattgcaa ttggcctcga ttttggcagt
    19081 gattctgtgc gagctttggc ggtggactgc gccagcggtg aagagatcgc caccagcgta
    19141 gagtggtatc cccgttggca aaaagggcaa ttttgtgatg ccccgaataa ccagttccgt
    19201 catcatccgc gtgactacat tgagtcaatg gaagcggcac tgaaaaccgt gcttgcagag
    19261 cttagcgtcg aacagcgcgc agctgtggtc gggattggcg ttgacagtac cggctcgacg
    19321 cccgcaccga ttgatgccga cggtaacgtg ctggcgctgc gcccggagtt tgccgaaaac
    19381 ccgaacgcga tgttcgtatt gtggaaagac cacactgcgg ttgaagaagc ggaagagatt
    19441 acccgtttgt gccacgcgcc gggcaacgtt gactactccc gctatattgg cggtatttat
    19501 tccagcgaat ggttctgggc aaaaatcctg catgtgactc gccaagacag cgccgtggcg
    19561 caatctgccg catcgtggat tgagctgtgc gactgggtgc cagctctgct ttccggtacc
    19621 acccgcccgc aggatattcg tcgcggacgt tgcagcgccg ggcataaatc tctgtggcac
    19681 gaaagctggg gcggcttgcc gccagccagt ttctttgatg agctggaccc gatcctcaat
    19741 cgccatttgc cttccccgct gttcactgac acctggactg ccgatattcc ggtgggcacc
    19801 ttatgcccgg aatgggcgca gcgtctcggc ctgcctgaaa gcgtggtgat ttccggcggc
    19861 gcgtttgact gccatatggg cgcagttggc gcaggcgcac agcctaacgc actggtaaaa
    19921 gttatcggta cttccacctg cgacattctg attgccgaca aacagagcgt tggcgagcgg
    19981 gcagttaaag gtatttgcgg tcaggttgat ggcagcgtgg tgcctggatt tatcggtctg
    20041 gaagcaggcc aatcggcgtt tggtgatatc tacgcctggt ttggtcgcgt actcggctgg
    20101 ccgctggaac agcttgccgc ccagcatccg gaactgaaag cgcaaatcaa cgccagccag
    20161 aaacaactgc ttccggcgct gaccgaagca tgggccaaaa atccgtctct ggatcacctg
    20221 ccggtggtgc tcgactggtt taacggtcgt cgcacgccaa acgctaacca acgcctgaaa
    20281 ggggtgatta ccgatcttaa cctcgctacc gacactccgc tgctgttcgg cggtttgatt
    20341 gctgccaccg cctttggcgc acgcgcaatc atggagtgct ttaccgatca ggggatcgcc
    20401 gtcaataacg tgatggcgct gggcggcatc gcgcggaaaa accaggtcat tatgcaggcc
    20461 tgctgcgacg tgctgaatcg cccgctgcaa attgttgcct ctgaccagtg ctgtgcgctc
    20521 ggtgcggcga tttttgctgc cgtcgccgcg aaagtgcacg cagacatccc atcagcccag
    20581 caaaaaatgg ccagtgcggt agagaaaacc ctgcaaccgc gcagcgaaca ggcacaacgc
    20641 tttgaacagc tttatcgccg ctatcagcaa tgggcgatga gcgccgaaca acactatctt
    20701 ccaacttccg ccccggcaca ggctgcccag gccgttgcga ctctataagg acacgataat
    20761 ggcgattttt gataattatg aagtgtggtt tgtcattggc agccagcatc tgtatggccc
    20821 ggaaaccctg cgtcaggtca cccaacatgc cgagcacgtt gttaatgcgc tgaatacgga
    20881 agcgaaactg ccctacaaac tggtgttgaa accgctgggc accacgccgg atgaaatcac
    20941 cgctatttgc cgcgacgcga attacgacga tcgttgcgct ggtctggtgg tgtggctgca
    21001 caccttctcc ccggccaaaa tgtggatcaa cggcctgacc atgctcaaca aaccgttgct
    21061 gcaattccac acccagttca acgcggcgct gccgtgggac agtatcgata tggactttat
    21121 gaacctgaac cagactgcac atggcggtcg cgagttcggc ttcattggcg cgcgtatgcg
    21181 tcagcaacat gccgtcgtta ccggtcactg gcaggataaa caagcccatg agcgtatcgg
    21241 ctcctggatg cgtcaggcgg tctctaaaca ggatacccgt catctgaaag tctgccgatt
    21301 tggcgataac atgcgtgaag tggcggtcac cgatggcgat aaagttgccg cacagatcaa
    21361 gttcggtttc tccgtcaata cctgggcggt tggcgatctg gtgcaggtgg tgaactccat
    21421 cagcgacggc gatgttaacg cgctggtcga tgagtacgaa agctgctaca ccatgacgcc
    21481 tgccacacaa atccacggcg aaaaacgaca gaacgtgctg gaagcggcgc gtattgagct
    21541 ggggatgaag cgtttcctgg aacaaggtgg cttccacgcg ttcaccacca cctttgaaga
    21601 tttgcacggt ctgaaacagc ttccaggtct gcccgtacag cgtctgatgc agcagggtta
    21661 cggctttgcg ggcgaaggcg actggaaaac cgccgccctg cttcgcatca tgaaggtgat
    21721 gtcaaccggt ctgcagggcg gcacctcctt tatggaggac tacacctatc acttcgagaa
    21781 aggtaatgac ctggtgctcg gctcccatat gctggaagtc tgcccgtcga ttgccgtaga
    21841 agagaaaccg atcctcgacg ttcagcatct cggtattggt ggtaaggacg atcctgcccg
    21901 actgatcttc aatacccaaa ccggtccagc gattgtcgcc agcctgattg atctcggcga
    21961 tcgttaccgt ctgctggtta actgtatcga cacggtgaaa acaccgcact ccctgccgaa
    22021 actgccggtg gcgaatgcac tgtggaaagc gcaaccggat ctgccaactg cttccgaagc
    22081 gtggatcctc gctggtggcg cgcaccatac cgtcttcagc catgcgctga acctcaacga
    22141 tatgcgccag ttcgccgaga tgcacgacat tgaaatcacg gtgattgata acgacacccg
    22201 cctgccagcg tttaaagacg cactgcgctg gaacgaagtg tattacggat ttcgtcgcta
    22261 agtagccaca tcaggtatgt aacgcctgat gcgacgctga cgcgtcttat ctggcctaca
    22321 cgctgcgatt ttgtaggccg gataagcaaa gcgcatccgg cattcaacgc ctgatgcgac
    22381 gctggcgcgt cttatcaggc ctacgcgctg cgattttgta gaccggataa gcgaagcgca
    22441 tccggcacga aggagtcaac atgttaaaag atctcaaacg ccaggtatta gaagccaacc
    22501 tggcgctgcc aaaacacaac ctagtcacgc tcacatgggg caacgtcagc gccgttgatc
    22561 gcgagcgcgg cgtctttgtg atcaaacctt ccggcgtcga ttacagcgtc atgaccgctg
    22621 acgatatggt cgtggttagc atcgaaaccg gtgaagtggt tgaaggtacg aaaaagccct
    22681 cctccgacac gccaactcac cggctgctct atcaggcatt cccctccatt ggcggcattg
    22741 tgcatacgca ctcgcgccac gccaccatct gggcgcaggc gggtcagtcg attccagcaa
    22801 ccggcaccac ccacgccgac tatttctacg gcaccattcc ctgcacccgc aaaatgaccg
    22861 acgcagaaat caacggcgaa tatgagtggg aaaccggtaa cgtcatcgta gaaacctttg
    22921 aaaaacaggg tatcgatgca gcgcaaatgc ccggcgttct ggtccattcc cacggcccgt
    22981 ttgcatgggg caaaaatgcc gaagatgcgg tgcataacgc catcgtgctg gaagaggtcg
    23041 cttatatggg gatattctgc cgtcagttag cgccgcagtt accggatatg cagcaaacgc
    23101 tgctggataa acactatctg cgtaagcatg gcgcgaaggc atattacggg cagtaatgac
    23161 tgtataaaac cacagccaat caaacgaaac caggctatac tcaagcctgg ttttttgatg
    23221 gattttcagc gtggcgcagg caggttttat cttaacccga cactggcggg acaccccgca
    23281 agggacagaa gtctccttct ggctggcgac ggacaacggg ccgttgcagg ttacgcttgc
    23341 accgcaagag tccgtggcgt ttattcacgc cgatcaggtt ccccgcgctc agcatatttt
    23401 gcagggtgaa caaggctttc gtctgacacc gctggcgtta aaggattttc accgccagcc
    23461 ggtgtatggc ctttactgtc gcgcccatcg ccaattgatg aattacgaaa agcgtctgcg
    23521 tgaaggtggc gttaccgtct acgaggccga tgtgcgcccg ccagaacgct atctgatgga
    23581 gcggtttatc acctcaccgg tgtgggtcga gggtgatatg cacaatggca ctatcgttaa
    23641 tgcccgtctg aaaccgcatc ccgactatcg tccgccgctc aagtgggttt ctatagatat
    23701 tgaaaccacc cgccacggtg agctgtactg catcggcctg gaaggctgcg ggcagcgcat
    23761 cgtttatatg ctggggccgg agaatggcga cgcctcctcg cttgatttcg aactggaata
    23821 cgtcgccagc cgcccgctgc tgctggaaaa actcaacgcc tggtttgcaa accacgatcc
    23881 cgatgtgatc atcggctgga acgtggtgca gttcgatctg cgaatgctgc aaaaacatgc
    23941 cgagcgttac cgtcttccgc tgcgtcttgg gcgcgataat agcgagctgg agtggcgcga
    24001 gcacggcttt aaaaacggcg tcttttttgc ccaggctaaa ggtcggctaa ttatcgacgg
    24061 tatcgaggcg ctgaaatccg cgttctggaa tttctcttca ttctcgctgg aaactgtcgc
    24121 tcaggagcta ttaggcgaag gaaaatctat cgataacccg tgggatcgaa tggacgaaat
    24181 tgaccgccgt ttcgccgaag ataaacctgc gctggcaact tataacctga aagattgcga
    24241 gctggtgacg cagatcttcc acaaaactga aatcatgcca tttttactcg aacgagcaac
    24301 ggtgaacggc ctgccggtgg atcggcacgg cggttcggtg gcagcgtttg gtcatctcta
    24361 ttttccgcga atgcatcgcg ctggttatgt cgcgcctaat ctcggcgaag tgccgccgca
    24421 cgccagccct ggcggctacg tgatggattc acggccaggg ctttatgatt cagtgctggt
    24481 gctggactat aaaagcctgt acccgtcgat catccgcacc tttctgattg atcccgtcgg
    24541 gctggttgaa ggcatggcgc aacctgatcc agagcacagt accgaaggtt ttctcgatgc
    24601 ctggttctca cgggagaaac attgcctgcc ggagattgtg actaacatct ggcacgggcg
    24661 cgatgaagcc aaacgtcagg gtaataagcc gctttcgcag gcgctgaaga tcattatgaa
    24721 tgccttttat ggcgtgctcg gcaccaccgc ctgccgcttc ttcgatccgc gcctggcatc
    24781 gtcgatcacc atgcgtggtc atcagatcat gcggcagacc aaagcattga ttgaagcgca
    24841 gggctacgac attatctacg gagataccga ctcgactttt gtctggctaa aaggtgcaca
    24901 ttcggaagaa gaggcggcga aaatcggtcg tgcactggtg cagcacgtta acgcctggtg
    24961 ggcggaaacg ctgcaaaaac aacggctgac cagcgcatta gaactggagt atgaaactca
    25021 tttctgccgt tttcttatgc caaccattcg cggagctgat accggcagta aaaagcgcta
    25081 cgccgggctg attcaggagg gcgacaagca gcggatggtg tttaaagggc tggaaaccgt
    25141 gcgcaccgac tggacgccgc tggcccagca gtttcagcag gagctgtacc tgcgtatctt
    25201 ccgcaacgag ccatatcagg agtacgtccg ccaaaccatc gacaaactga tggcgggtga
    25261 actggatgcg cggctggttt accgtaaacg ccttcgccgt ccgctgagcg aatatcagcg
    25321 taatgttccg cctcatgtac gcgccgctcg ccttgccgat gaagaaaatc aaaagcgcgg
    25381 tcgccccttg caatatcaga atcgcggcac cattaagtac gtatggacca ccaacggccc
    25441 ggagccgctg gactaccaac gttcaccact ggattacgaa cactatctga cccgccagct
    25501 acaacccgtg gcggagggaa tactcccttt tattgaggat aattttgcta cacttatgac
    25561 cgggcaactt gggctatttt gagcaaaaaa aagagttcgc cagataccat tttgatgcgt
    25621 gacgaatgct ttgccatcca gtaccatagc gccctttcca ttcctggacc tgaataacac
    25681 cactacctca taagcacggt agcgggtggt tattgcctgc aattaaagat atagagccga
    25741 acacatatgc cttttacact tggtcaacgc tggatcagcg atacagaaag cgaattggga
    25801 cttggaaccg ttgtcgcggt ggatgcgcga actgtcactt tacttttccc atctactggt
    25861 gaaaaccgtc tgtacgcacg cagtgattcc cccgtgaccc gcgtgatgtt caaccctggt
    25921 gataccatta ccagccatga cggctggcag atgcaagtcg aagaagtaaa agaagaaaat
    25981 ggcttgctga cctatatcgg tactcgcctg gatactgaag agtccggcgt agccctgcgt
    26041 gaagttttcc ttgatagcaa actggtgttc agcaaaccgc aggaccgtct gtttgccggg
    26101 cagattgacc gtatggaccg ctttgcgctg cgttatcgcg cgcgtaagta ttccagcgaa
    26161 cagttccgta tgccgtacag cggcctgcgc ggtcagcgta ccagcctgat cccgcatcag
    26221 ctcaacatcg ctcatgatgt tggtcgccgc cacgcgccgc gcgtcctgct ggctgacgaa
    26281 gtgggtttag ggaaaaccat tgaagccggg atgatcctgc atcagcaact gctctctggc
    26341 gctgctgaac gtgtgctgat tatcgtcccg gaaaccttac agcatcagtg gctggtagaa
    26401 atgctgcgcc gtttcaacct gcgctttgct ctgtttgatg atgagcgtta tgccgaagct
    26461 cagcacgatg cttacaaccc gtttgacacc gaacagctgg tgatttgctc gctggatttt
    26521 gcccgtcgta gcaaacagcg cttggaacat ctctgtgaag ccgaatggga cctgctggtg
    26581 gtcgatgaag cgcatcacct ggtgtggagc gaagatgcgc caagccgtga atatcaggcc
    26641 attgaacaac tggcagagca cgtgccgggc gttctgctgc tgaccgcgac cccggaacag
    26701 ctggggatgg aaagccactt cgcccgtctg cgtctgctgg acccgaaccg tttccacgat
    26761 tttgcgcagt tcgttgaaga gcagaaaaat tatcgtccgg ttgcggacgc cgttgccatg
    26821 ctgctggcag gtaacaaact gagcaatgac gaactgaaca tgctcggcga gatgatcggc
    26881 gagcaggata tcgagccgct gttgcaggca gcaaacagcg acagcgaaga tgcccagagc
    26941 gcccgtcagg agctggtttc gatgctgatg gatcgccacg gcaccagccg cgtgctgttc
    27001 cgtaacacgc gtaacggtgt gaaaggattc ccgaaacgcg agctgcacac cattaagctg
    27061 ccgctaccga cgcagtatca gacggctatt aaagtctccg gcattatggg cgcacgtaaa
    27121 agttcggaag atcgtgctcg cgatatgctc tacccggagc gtatttatca ggaatttgaa
    27181 ggtgataacg ccacctggtg gaacttcgat ccgcgcgttg agtggctgat gggctacctg
    27241 accagccatc gctctcagaa agtgctggtg atctgcgcca aagctgccac tgcgctgcaa
    27301 ctggagcagg tactgcgcga acgtgaaggt attcgcgctg cggtgttcca cgaaggtatg
    27361 tcgattatcg aacgtgaccg cgctgccgcc tggtttgccg aagaagacac cggcgcacag
    27421 gtactgctgt gctcagaaat cggttctgaa ggacgtaact ttcagttcgc cagccacatg
    27481 gtgatgtttg acctgccatt caacccggat ctactggagc agcgtattgg tcgtctggat
    27541 cgtatcggcc aggcgcacga tattcagatc catgtgcctt atctggagaa aaccgctcag
    27601 tcggtgctgg tgcgctggta tcacgaaggt ctggatgcat ttgagcacac ctgcccgacc
    27661 ggacgcacta tttacgatag cgtatacaac gatctgatta actatctggc ttcaccggat
    27721 caaaccgaag gctttgacga tctgatcaaa agctgccgcg agcaacatga agcgctgaaa
    27781 gcacagctgg aacagggtcg tgaccgcctg ctggaaatcc actccaacgg tggcgaaaaa
    27841 gcccaggaac tggcagaaag cattgaagag caggatgacg ataccaacct gatcgccttc
    27901 gccatgaacc tgttcgatat tatcggtatc aatcaggacg atcgcggcga caacatgatc
    27961 gtgctgacgc cgtccgatca tatgctggtg ccggacttcc ctggcctgtc ggaagatggc
    28021 atcaccatca cctttgatcg tgaagtggcg ctggcgcgtg aagatgcaca gtttattacc
    28081 tgggagcatc cgctgatccg caacggtctg gatctgatcc tttctggcga taccggtagc
    28141 agcacgattt cactgttaaa aaacaaagcg ttgccggtag gtacgctgtt ggttgaactg
    28201 atttatgtgg ttgaagccca ggctccgaag cagttgcagc tcaaccgctt cctgccaccg
    28261 acgccggtac gtatgctgct ggataaaaac ggcaacaacc tggcggcgca ggtagagttt
    28321 gaaaccttta accgccagct taacgcggtt aaccgtcaca ccggcagcaa actggttaac
    28381 gccgtgcagc aggatgttca cactatcctt caactgggtg aagcgcagat cgagaaatct
    28441 gcccgtgcat tgattgatgc agcgcgtaac gaagccgacg aaaaactgtc tgccgagctg
    28501 tctcgtctgg aagctctgcg tgcagtgaac ccgaacattc gtgacgacga actgaccgcc
    28561 attgagagca accgtcagca ggtaatggaa agcctggatc aggcaggttg gcgtctggat
    28621 gccctgcgtt tgatcgttgt aacgcatcag taacggagcc gaaaatgggg atggaaaact
    28681 acaatccacc gcaggaaccc tggttggtta tcctgtatca ggatgaccat attatggtgg
    28741 tcaacaagcc gagcggtttg ttgtcagtgc cgggtcgtct ggaagagcac aaagacagcg
    28801 tgatgacgcg cattcagcgt gattatccgc aggcagaatc ggtgcatcgt ctggatatgg
    28861 ctaccagcgg cgtgattgta gtggcgctga ccaaagccgc ggagcgggag ttaaaacgcc
    28921 agttccgcga gcgcgagccg aaaaagcagt atgtggcccg cgtctggggg catccatccc
    28981 ccgcagaagg tctggtggat ctgccgctga tttgcgactg gccaaaccgc ccgaaacaga
    29041 aagtctgtta cgaaacgggt aaacctgcgc agacggaata tgaagtggtg gagtatgcgg
    29101 cggataacac ggcaagagtg gtgttaaaac cgattaccgg gcgttcgcat caattgcgtg
    29161 tgcatatgct ggcgctgggg catccgattc tcggcgatcg tttttatgca tcaccagaag
    29221 cgagagcgat ggcaccgcgt ttgttgctac atgcagagat gttgacgatt acccatccgg
    29281 cgtatggcaa tagtatgacg tttaaagcgc cagcggattt ttaatcgtca cgctggcgca
    29341 gttcggtagg cggataaggc gttcacgccg catccggcaa ttgtgcgccg attgcctgat
    29401 gcgacgcggg ccgcgtctta tcaggcctac agtttaaaac ttatttaaac cctttctgct
    29461 gctttatcag ctcatatgcc tgctgaattt cctgcgcttt ctgcttcgcc atctccatca
    29521 tctcaggcgg caaacctttc gccaccagct tatcgggatg gtgttcactc atcagcttac
    29581 ggtaggcacg tttgatggtg gtcgcatcat ccgtcggctt cacgcccagc acattacagg
    29641 catcttccag cgtcgggcca cgctgcgctt gctgccagtt accaccgcca gattgctgct
    29701 gataaccgcc gccaaactgt gcaccgccct gcatcatgcg caaaaactgg tcaaactggg
    29761 cgcgggagat ccctaattct tcagcaatga catacagcac cgcccgttca ttcgggtgca
    29821 gtgaaccatc agcaaacgcc gcctgaatct ggatctccag aaacatacga attaagtcaa
    29881 aacgaccaaa gcagacactg cgaaactggc gcatcttttc gcgcagcggg taattatctg
    29941 attttcccac ccggaacgcg ttttgcgccg cagtacggga agcgccatga aggttcattc
    30001 ggtccatcaa ctggctggcg atatgaatat cagcctccgt gacgcgacct ttggatttgg
    30061 ttaaatgccc catcacttca aaagtggtgg caaaaaacag cgcctgacgc tcacgctggt
    30121 tggcgaacca cgccatttta cggctacggg ctttatcaaa catatggcca attaacaggc
    30181 ctaacactac gccccaaaag ccgccgccca tcagtaaggc cacggccacg ccaatgattt
    30241 ttccccaata ctgcatatat tccccaaatc gacacacgga tatcagggct atctcccaca
    30301 atataaaggt gcttttaccg ttttccggct gcggtcaatt gtgggacatc gcctataatt
    30361 tgcattatca tacctgtcat tcaatgccgt gcctaacacc actgacgcta ttcggacagg
    30421 attaacacta gcgtagagat gacgagtacg ttagtctctg accgtttgtc acgcgcaacg
    30481 ttaccgatga tggaacaata aaatcaacgt atgaaaaaac gtatccccac tctcctggcc
    30541 accatgattg ccaccgccct ttatagtcaa cagggactgg cagccgacct cgcctcacag
    30601 tgcatgttgg gcgtgccaag ctatgaccgt cctctggtac agggcgatac caatgactta
    30661 cccgtgacta tcaatgctga ccacgcgaaa ggggactacc cggatgacgc cgtgtttact
    30721 ggcagcgtgg atatcatgca gggtaacagc cgtctgcagg ccgacgaagt gcagctccat
    30781 caaaaagagg caccaggaca accggagccg gtacgtaccg ttgatgcgct cggtaatgtc
    30841 cattacgacg ataaccaggt gatcctcaaa gggccgaaag gctgggcgaa tctgaacacc
    30901 aaagatacca acgtctggga aggtgattac cagatggtgg gtcgccaggg tcgcggtaaa
    30961 gcggacctga tgaaacaacg tggcgaaaac cgctatacca ttctggataa cggtagcttt
    31021 acctcctgtc tgccgggttc tgacacctgg agcgtggtag gtagcgaaat tattcatgac
    31081 cgcgaagaac aagttgcgga gatctggaac gcccgcttta aggtgggtcc ggtaccgatc
    31141 ttttatagcc cctatttgca gttgccggtg ggtgacaaac gtcgctctgg tttcttgatc
    31201 ccgaacgcca agtacaccac caccaactac tttgagttct acctgccata ttactggaac
    31261 atcgcgccaa atatggatgc caccatcacg ccgcattata tgcatcgtcg tggcaacatc
    31321 atgtgggaga acgaattccg ctacctctcc caggcgggcg ctggcttgat ggaactggac
    31381 tatctgcctt cagataaagt ctatgaagat gaacacccga acgatgacag ttcacgtcgt
    31441 tggttattct actggaacca ctccggggtc atggatcagg tgtggcgttt caacgtcgac
    31501 tacaccaagg tcagcgatcc tagctacttc aatgatttcg ataacaagta cggttccagt
    31561 actgacggct acgcaacgca aaaattcagc gttggctatg cggtgcaaaa cttcaatgcc
    31621 accgtttcaa ccaagcagtt ccaggttttc agcgaacaga acaccagtag ctactcggca
    31681 gagccgcagt tagacgttaa ttactaccag aatgatgttg gtccgtttga tacgcgtatt
    31741 tacggccagg cagtgcactt tgttaacacc agagacgaca tgcctgaagc aacccgtgtt
    31801 cacctggaac cgaccatcaa tttgccgctc tctaataact ggggcagcat caataccgaa
    31861 gcgaagtttc tggcaaccca ttatcagcaa accaatcttg actggtataa ctccagaaac
    31921 acgaccaagc tggacgaatc cgttaaccgc gtaatgccgc aattcaaagt tgacggcaaa
    31981 atggtctttg aacgcgatat ggaaatgctg gctccgggtt atacccaaac gctggaaccg
    32041 cgcgcgcagt atttgtacgt gccgtatcgc gatcagagcg acatctataa ctacgactcg
    32101 tctctgctgc aatctgacta ctctggcctg ttccgggacc ggacttacgg cggtcttgac
    32161 cgtattgcct ccgctaacca ggtgacgacc ggtgtcacat ctcgcatata tgatgatgct
    32221 gccgttgaac gttttaatat ttccgttggt caaatctact atttcacgga gtctcgcact
    32281 ggcgatgaca acataacatg ggagaatgac gacaaaacgg gctcactggt gtgggcaggc
    32341 gacacctact ggcgtatctc cgagcgttgg ggattgcgtg gcgggattca gtacgataca
    32401 cgtctggata acgtagcgac cagtaactcc agcattgaat accgtcggga tgaagaccgt
    32461 ctggtacagc tgaattacca ttacgccagc ccggaatata ttcaggctac gctgcctaag
    32521 tactattcca ctgctgagca atataagaat ggtatttcgc aggtaggtgc tgtcgccagc
    32581 aggccaattg ccgatcgttg gtccattgtt ggggcctact actacgacac caatgcgaac
    32641 aagcaagccg actctatgtt aggtgtgcaa tacagctcct gctgctatgc aattcgcgtc
    32701 ggttacgagc ggaagctgaa cggttgggat aacgataaac aacatgcggt atatgacaac
    32761 gcaatcggct ttaacatcga acttcgcggc ctgagctcca actacggtct gggtacgcaa
    32821 gagatgctgc gttcgaacat tctgccgtat caaaacactt tgtgatttgt tgatttacca
    32881 cgtaatccgc agtgcggtta attgaaatgg aaaaagtatg aagaactgga aaacgctgct
    32941 tctcggtatc gccatgatcg cgaataccag tttcgctgcc ccccaggtag tcgataaagt
    33001 cgcagccgtc gtcaataacg gcgtcgtgct ggaaagcgac gttgatggat taatgcagtc
    33061 ggtaaaactg aacgctgcgc aggcaaggca gcagcttcct gatgacgcga cgctgcgcca
    33121 ccaaatcatg gaacgtttga tcatggatca aatcatcctg cagatggggc agaaaatggg
    33181 agtgaaaatc tccgatgagc agctggatca ggcgattgct aacatcgcga aacagaacaa
    33241 catgacgctg gatcagatgc gcagccgtct ggcttacgat ggtctgaact acaacactta
    33301 tcgtaaccag atccgcaaag agatgattat ctctgaagtg cgtaacaacg aggtgcgtcg
    33361 tcgcatcacc atcctgccac aggaagtcga atccctggcg cagcaggtgg gtaaccaaaa
    33421 cgacgccagc actgagctga acctgagcca catcctgatc ccgctgccgg aaaacccgac
    33481 ctctgatcag gtgaacgaag cggaaagcca ggcgcgcgcc attgtcgatc aggcgcgtaa
    33541 cggcgctgat ttcggtaagc tggcgattgc tcattctgcc gaccagcagg cgctgaacgg
    33601 cggccagatg gggtggggcc gtattcagga gttgccgggg atcttcgccc aggcattaag
    33661 caccgcgaag aaaggcgaca ttgttggccc gattcgttcc ggcgttggct tccatattct
    33721 gaaagttaac gacctgcgcg gcgaaagcaa aaatatctcg gtgaccgaag ttcatgctcg
    33781 ccatattctg ctgaaaccgt cgccgatcat gactgacgaa caggcccgcg tgaaactgga
    33841 gcaaattgct gctgatatca agagtggtaa aacgactttt gctgccgcag cgaaagagtt
    33901 ctcgcaggat ccaggttctg ctaaccaggg cggtgatctc ggctgggcta caccagatat
    33961 tttcgatcca gccttccgtg acgccctgac ccgcctgaac aaaggtcaaa tgagtgcacc
    34021 ggttcactct tcattcggct ggcatttaat cgaactgctg gatacccgta atgtcgataa
    34081 aaccgacgct gcgcagaaag atcgtgcata ccgcatgctg atgaaccgta agttctcgga
    34141 agaagcagca agctggatgc aggaacaacg tgccagcgcc tacgttaaaa tcctgagcaa
    34201 ctaatggtta aaacccaacg tgttgtgatc actcccggcg agcccgccgg gattggcccg
    34261 gacttagttg tccagcttgc acagcgtgag tggccggtcg aactggttgt ttgtgccgat
    34321 gccactctcc ttaccgaccg ggcagcgatg ctcggtttgc cgctcaccct ccgcccttat
    34381 tcccccaact cccctgcaca accgcaaact acgggcacat taacgctact tcctgtcgcg
    34441 ctacgtgaat ctgtcactgc ggggcagtta gcgattgaaa atgggcatta cgtggtggag
    34501 acgctggcgc gagcgtgtga tggctgtctg aacggtgaat ttgctgcgct gatcacaggc
    34561 cccgtgcata aaggcgtcat taacgacgca agcattccgt ttaccggtca taccgagttt
    34621 ttcgaagagc gttcgcaggc gaaaaaagtg gtgatgatgc tggcgaccga agaacttcgc
    34681 gtggcgctgg caacgacgca tttaccgctg cgcgatatcg cagatgctat cacccctgca
    34741 cttttgcacg aagtgattgc tattttgcat cacgatttgc gtacaaaatt tggtattgcc
    34801 gaaccgcgca ttctggtctg cgggctgaat ccgcacgcgg gcgaaggcgg tcatatgggt
    34861 acggaagaga tagacaccat tattccggtg ctcgacgagc tgcgggcgca ggggatgaaa
    34921 ctcaacgggc cgctgcctgc cgataccctg tttcagccga aatatctcga taacgccgac
    34981 gccgtgctgg cgatgtacca cgatcagggt cttcccgtgc taaaatacca gggcttcggg
    35041 cgcggtgtga acattacgct gggcctgccc tttattcgca catcagtgga ccacggcacc
    35101 gcgcttgaac tggcgggacg tggcgaagcc gatgtcggca gttttattac ggcgcttaat
    35161 ctcgccatca aaatgattgt taacacccaa tgaataatcg agtccaccag ggccacttag
    35221 cccgtaaacg cttcgggcaa aactttctca acgatcagtt cgtgatcgac agcattgttt
    35281 ctgccattaa cccgcaaaag ggccaggcaa tggtcgaaat cggccccggt ctggcggcat
    35341 tgaccgagcc ggtcggcgaa cgtctggacc agctgacggt catcgaactt gaccgcgatc
    35401 tggcggcacg tctgcaaacg catccattct taggcccgaa actgacgatt tatcagcagg
    35461 atgcgatgac ctttaacttt ggcgaactgg ccgagaaaat gggtcagccg ctgcgtgttt
    35521 tcggcaacct gccttataac atctccacgc cgttgatgtt ccatctgttt agctatactg
    35581 atgccattgc cgacatgcac tttatgttgc aaaaagaggt ggtgaatcgt ctggttgcag
    35641 gaccgaacag caaagcgtat ggtcgattaa gcgtcatggc gcaatactat tgcaacgtga
    35701 tcccggtact ggaagttccg ccgtcagcct ttacaccacc acccaaagtg gattccgccg
    35761 tcgtgcgcct ggtccctcat gcaacgatgc ctcacccggt taaagatgtt cgcgtgttga
    35821 gccgcatcac caccgaagcc tttaaccagc gtcgtaaaac cattcgtaac agcctcggca
    35881 acctgtttag cgtcgaggtg ttaaccggaa tggggatcga cccggcgatg cgagcggaaa
    35941 atatctctgt cgcgcaatat tgccagatgg cgaactatct ggcggagaac gcgcctttgc
    36001 aggagagtta acgatgatca attcgccccg agtgtgtatt caggttcaaa gcgtctacat
    36061 tgaggcacaa tcttcacctg ataatgaacg ttacgttttt gcttataccg taaccatacg
    36121 caatctgggg cgagcgccag tgcagttgtt ggggcgttac tggctgatca ccaatggcaa
    36181 tggtcgtgaa accgaagtcc agggcgaagg agtggttggc gtccagccac ttatcgcgcc
    36241 tggcgaagag taccagtaca ccagcggtac aatcattgaa accccgctgg gcaccatgca
    36301 gggtcactac gaaatgatcg atgaaaatgg cgtccctttc agcatcgaca ttcccgtatt
    36361 ccgactcgcc gttcccacac tcattcatta aaagaatatg gcgacatacc ttattggcga
    36421 cgttcatggt tgttacgatg aactgatcgc attgctgcat aaagtagaat ttacccccgg
    36481 gaaagatacc ctctggctga cgggcgatct ggtcgcgcgc ggcccgggtt cgctggatgt
    36541 tctgcgctat gtgaaatcct taggcgacag cgtacgtctg gtgctgggca atcacgatct
    36601 gcatctgctg gcggtatttg ccgggatcag ccgcaataaa ccgaaagatc gcctgacacc
    36661 gctgctggaa gcgccggatg ccgacgagct gcttaactgg ctgcgtcgcc agcctctgct
    36721 gcaaatcgac gaagagaaaa agctggtgat ggcccacgca gggatcacgc cgcagtggga
    36781 tctgcagacc gccaaagagt gcgcgcgcga tgtagaagcg gtgctgtcga gtgactccta
    36841 tcccttcttt cttgacgcta tgtacggcga tatgccaaat aactggtcac cggaattgcg
    36901 ggggctggga agactgcgtt ttatcaccaa cgcctttacc cgtatgcgtt tttgcttccc
    36961 gaacggtcaa ctggatatgt acagcaaaga atcgccggaa gaagcccctg ccccactgaa
    37021 accgtggttt gcgattcctg gccctgtcgc tgaagaatac agcatcgcct ttggtcactg
    37081 ggcatcgctg gaaggcaaag gtacgccgga aggtatttac gcgctggata ccggctgctg
    37141 ctggggtggg tcattaacct gcctgcgctg ggaagataaa cagtattttg tccagccgtc
    37201 gaaccggcat aaggatttgg gcgaagcggc ggcgtcttaa acacagcctg atacaggaag
    37261 gccggataag acgcgaccgg cgtcgcatcc ggcactagcc gtaaattcta tacaaactta
    37321 ccgccgctcc agaatctcaa agcaatagct gtgagagttc tgcgcatcgg cgtcgtggaa
    37381 ttcgctgaat accgattccc agtcatccgg ctcgtaatcc gggaaatggg tgtcgccttc
    37441 cacttctgcg tcgatatgcg tcagatacag tttttgcgct tttggcagga actgctcata
    37501 aacgcgaccg ccgccaatca ccatgatttc tggtacgtca ccacacgccg caatggcttc
    37561 atccaccgat ttcacccacg ttacgcgatc gtccgtaccc ggttgactgc tgaggataat
    37621 atttttgcgt cctggcaacg gacgaccgat tgattcccaa gtatggcggc ccataatcac
    37681 gggtttattt aaggtgttgc gtttaaacca ggcgagatcg gcaggcaggt tccacggcat
    37741 ggcgttttcc atgccgataa cgcgatctac cgctaacgcc gcaatcagac tgatcattga
    37801 gatttcccga taaaaaaatt gtcgccacta tacgtaaagc gtaaaccgtc gtcgactggt
    37861 gcgaggatga tgttgaggaa aattttatat tctgctggcg agtccacgct ctccccctgg
    37921 actcgccgca ttacaatgaa acaaaaacaa acagttagct gtaaagtgtg atttacgtca
    37981 ctctttatta agatgagggt ttcgtttccg gttcatccgc catgttgccg gtatgtttac
    38041 cttcttctgt tccctgccag ccatgtcgtt gaattaatga cagatgttcg cggtcctcgg
    38101 tgatgatctc acttaacatc gcgctggtgc gtttatagac cgccgcgcgg gctttggtgt
    38161 cattctcaac cattgccatc tcttccacca tctgaatatt aaagcggcgg aacacatcgg
    38221 cacgttctcg cgcttcatac ggccccagac ctaaactttc cagcgccaga cgcccggttt
    38281 tcagcgcacc ttcaaaggtt tcacgctccg gcttttcaac gcctgcctga cgcaaacgaa
    38341 tgtagtgatc gacatcgcgg gcgcgggcaa taatctgcaa atgcgggaaa tgttctttca
    38401 ccatctccgt cagttgcagg ttggtttgcg gatcgtcaat agcgttaatc agcacttccg
    38461 ctttcgccgc tccggcagat tccagtaaat ccatccgcgt ggcatcgcca taaaacactt
    38521 tcataccaaa tttacgcagc gtttcgatat ggtccggatc gtgatcgagc accaccattt
    38581 tcaccccgct ggagagcagt aaacgtccgg taatctgtcc aaaacgaccg aatccggcga
    38641 taatcacgcg cggctgttct tcatcgatct catcggcttc acgcgcctcc tccgtagaag
    38701 attgctcaag gcgattgagg atcaccagca gaatcggcgt tgctgccatc gacagcgcca
    38761 ccgccagggt cagcgatttc gcccactccg gctccagcac attcgccatc tgcgccgcac
    38821 caaataccac aaaggcaaac tcactgccct gccctaacaa caccgcaaac cagcgacgct
    38881 gtttatttgg cacctgcaac ggtcgggcaa tcagccacag catggcgatt ttgatgatga
    38941 ggaaaccgag cagcaaaatg acaatgcgca atgggttttc aatcagcgtg ccaaagtcta
    39001 tcgacatgcc aacaccgatg aaaaacagcc ccaacagcaa acctttaaac ggttcgatat
    39061 cgctttccaa cgcatgacgg tattccgagc ttgccagcag tacgcccgcc agaaacgcgc
    39121 ccatcgccat cgacaagccg acctcttcca gcagcaaacc aaagccaaac acaaggaata
    39181 acgccacggc actaaacact tcccgcaagc cagagcgggc aacaaaacgc agcgccggac
    39241 gcgtaacata gcgccccagc aataccacca gtaccagcgc acccgccact tttaacgccg
    39301 agagagcaaa tgcgcccatc gtcgtcgagg cactgctcgc cgccagtagc gggatcatcg
    39361 ccaccagcgg gattgccgcg atatcctgga acagcagcac tgcaaaggca ctgcggccca
    39421 tttgcgtcac catcagattg cgttcattca tcgcctgcat ggcaatcgcc gttgatgaga
    39481 gcgccagcgt cataccgatc aattccgcga cctgccagcg cagcccaagt aacatacaga
    39541 acagccccag caggccaccg caaatcacca tctgcaatgc gccaccgccg aacactgccg
    39601 cacgcagctt ccacagcctt tgtggatcga gttcgaggcc gataataaac agcatcagca
    39661 ccaccccaat ctcggcaaag tgcagaatag attcggcatc ggtcaccagt cgcagccccc
    39721 acgggccaat aatgcagccg gcgatcaggt agccaagtac cgatcccaga ccaagacgta
    39781 ccgcaatggg tacaatcagc gccgccgatc cgaggtagat cagcgcctga accagcgtat
    39841 gactatccat gatgggcctc ctgccattcc agcagacgtt gcttatagtg gcgcgcctgc
    39901 ccttcgaggg tttcgtcgtc acaaataaag gtgcagtgca tggcaaacgg tggcagccag
    39961 ttcagcccgc agtagattgc cgtcgcctgt agcggctgcg acagcacatc aaagcccgga
    40021 tgcgcaccaa tttcaaaatg gctttccccg ccgccggtcg tcaccgccca cagcaaatgt
    40081 ttgccatgca gcgccgtgcc gccgtgaccg taagcccagc cgtgcgacaa aactttatcg
    40141 atccaaagtt taagaagcgg aggaatgctg taccactgca tcgggtgctg ccagacgatc
    40201 agatcggcgc gagagagcgc ctcctgctcg gcggcaatat cgatattgaa gtcaggatag
    40261 agttgataaa gagagcgaat ttcgacgcct tccagcgtcc ttgcctgttc aagcatccgt
    40321 ttattcgcat gggaatgatg cggatacgga tgcgcataaa tgatgaggat catagttatt
    40381 agcccgcgtg tttacgcctt tgttttccca caaagtgtag tcatatattc acgcggctta
    40441 tagtcaataa tattgattca gaaaagagag aaatctgaac aaataatccc attctctgcc
    40501 cgcggatcgt catattgctg gtctgcgcca gtgacattcc tcgagtctcc ggggcaaacg
    40561 ctacggaaat caacaagcca aacagcgaga tacccgcccc cattagcatc gtgttactga
    40621 taccgtaatt attgataaat atcggcagtg cccaggtcga aacaatggtg ccaatacgac
    40681 ttaaggacat aatcacgccc acggcagagg cgcggatatc cgtcgggaag agttcattag
    40741 gatagagcca ctgcaaatta cccggcccgc cagagaaaaa ggcatacacc gcaaaggcca
    40801 tcactaccag ccagatcccc atatccggga ttagccccaa aaccgccagc gccagcgtca
    40861 tcatggcaaa gctgccaatc aacaatggac gccgtccggc agtgtttagc cacagcatcg
    40921 gcggaataca gccgagcata aagaacaggc taatcaccac attccctagt gccgcgtttt
    40981 tgccaacccc caatcccaac gaaccaacga tttgcgggcc aaaggtgtaa atggcgaaca
    41041 ttgggatcac ctggcaggtc cagatggcgg caacaaacag aacaaaagga aaatggcggc
    41101 gattaaacag atcgcgaaaa cgggtttgct gcggctgctc ttcatcgaaa gccaccggtt
    41161 cgccaaacag ttttatcatc atctcttcgc actcttttac tcgccctttg cgtaataacc
    41221 agcggggaga ttctggcagt tcgaatcgac caatcaaaat caacaaacaa gggatcgccg
    41281 cgctacccag catccagcgc caaccacctt ccacatcata aagccagtag ccgaccagat
    41341 cggcgcaggt cgcgccgaca taccacatcg cggcaataaa gctgatggaa aacgcccgct
    41401 gacgggtact ggagaactcg gtgatcatcg aagtggcgat gggataatct gcaccgatga
    41461 caatgccgat gagtacccgc atcaccaaca gttcgacggg ggatgaaaca cacatcgtcg
    41521 ccaccgatat cacgccgatg gcgatgatat caatgaggaa cattttgcgc cgtccgactt
    41581 tatcggagat atagccaaac agcgatgtgc caacgaacag cccggcgagc gttcccgcgc
    41641 ccagcaagcc aatccagtca gcgtccagtt tcagcgccgg agtcagttgc tccagcgcca
    41701 cgccaatcat taccagtaca taaccatcca gaaacggacc accgcttccc cacagcaaaa
    41761 tgcggcggtg aatagaggag aatttgagat cgtcaaagtt tctggacggt tgcatgggtt
    41821 attcctgttt ttttgcaatg agagtcagta cggcgttacg tatcaaacca acatcagccg
    41881 taacggaact ccacaccaaa ggtgccgcgc gggtattccc actgttccag cgccgacccc
    41941 agccccagaa tgcgacaggt gccgcactcc agacatccgg cgtaatcgaa gcgcacactg
    42001 ccgtcatcct gcttcttgta cagacctgcg gggcacgctt tcaccagcag ctccagcacc
    42061 tgtttatcag catcggcctt cacaacaatg tgcggatgct cttcatcgac attgaattta
    42121 ttgacgccca gtttgacgtc cacattgacg ggagaagtca taaaacggtc actcctttca
    42181 tgccatcctt gatcagattg atgaagccca ctttcttgcc gtggcggagg attttcttgc
    42241 gcatcagttc cggcgcactg ccgtcaatgg tgaacaggtc acgcgccacg cccaccgcca
    42301 gttccgggtt gccgctaaac atgcgtgggt tatcaaggaa cgccggtagt ttctggtaca
    42361 tgcgcatatc gcgcagcggg ccactctcca gatgctgacg atattccgcg agtttttgct
    42421 tactgaaatc gtcgcttttc atcgctgaaa gcacggtttt tgctgcagct tccccggcgg
    42481 caatcgccag atccataccg cgaatggtaa aaccgaggtt catacacatt ccggcggcat
    42541 caccggcaat caatacgccg tcaccgacca gctccggcag catgttgatg cctgcttccg
    42601 gcactacgtg agcggaatat tccaccagct tgccgcccgc gatcagcggt gcaacggccg
    42661 gatgctgttt gaaatcttcc agcatttgcg gcaccgattt tttcgcgtca tgcagatgat
    42721 gcagaccaca aaccagcccc agcgacaggg tattttcatt ggtataaagg aagccgccgc
    42781 ccatcaggcc atcggtgggt gatcccgcaa acagacaagc cgcgccctga ttaccctgca
    42841 actgaaaacg gtcttcgatt accgacttcg gtaactcgat cagttccttc acgccaaccg
    42901 ccacatccgt cggtttaacg cgttttgcca tccccaactt ttcggcaagg atggagttca
    42961 ccccatcagc aaggagcacc gttttcgctt caatcacatc gccatcggct tctacaccga
    43021 cgactttgcc atcgcgctgt acgaggttat cgacgcggat cccggtaatt aactgcgcgc
    43081 ccgcttcttc ggcctgctcc atcaaccagg catcaaattt actgcgcaaa acggagtaag
    43141 aacgctggga tggcgaggtt tcgtcaccgt tgcagtagtc catagtcatc gctgactttt
    43201 ccgtcataaa cgcgagtttt tcatgggtga tcaggcgttc tacgggggcg gaatcggcga
    43261 aacctggaat aatgtgttcc agactgtggg catagagacg cccgccagta acgttcttgg
    43321 cacctgcgga attgccacgc tcgataacta acacttgcgc cccttcgcgg gcgagcacca
    43381 gtgcggcaac cgaaccggca agccctgcac cgacgatgat ggcgtcaaag atatcttcgg
    43441 acataagagc tccctgtcag cggcagagcc gtaatgcagc cctgccagag tggatcaacg
    43501 cgctaaagct gcggtcagtg ccggaaggat cttcacggca tcgccaacaa tgccgtaatc
    43561 cgcgtactgg aagatcggcg catttttatc tttattgatg gcgaaaatgg tttgcgacgc
    43621 gttagcgcca accatgtgct ggatctaccc ggagatcccc accgccaggt acagttcagg
    43681 tttcagcatc aggttggaga taccgacata gcgttcgtgc tccatccatt tttcgttttc
    43741 tgccaccgga cgagaacagg ccaactccgc acctatcgcc ttgcaaagct gttctgccag
    43801 cgcaatgttc tctttgctgc caatgccgcg accgacgctg accaccagac gggctttgtc
    43861 gagatcgacg ctgttgctct ggcgcgcctg ggttgccgtg cgggtaatcg ccacagccgg
    43921 agcctgccac tccacggtgt gcgtttcgcc agtgcgtgac gcgtctggct gagccgcatc
    43981 gaacgtgccg ctgctgatgg tcagtaccgc atacggcgtg gcaatgcgtt cttcgccaat
    44041 cgccagaccg ccgtaaacca tgtgtttcac tgttgcttta ccgtcctgta cgctgacggt
    44101 gctggcatcg ttagacaccg ccgctttaag gcgataaccc agttttgccg ccagtaattt
    44161 gccgcgacgg gtgtttggca acagcaccag accgtcagcg ccgtgctggc gaatagtgtc
    44221 agccatgaca ccggcgtaat cttcgatcat ccgatcgtcc ggtttgccgt ttaatttcca
    44281 gacatgatta gcgccgagct ggattgcctg tgcgccgtcg gcatcattga ggacaaaggc
    44341 attgatttga ttagctaaag cctgcgcacc gttcatcagt tccggcagac gagagggggt
    44401 atcgctgaat acccagactt gagaaaacgt gttcatagca tcccctgtaa ttaaatgact
    44461 ttgcgaagat tttcagcaaa tgcggcgatc tgttcttcgc cgtcgccttc aatcacgatg
    44521 cgctgacgct cgcgctgttt cggcgcggca acctgttgtt cagaccaggc gtccacggcg
    44581 ttaaaaccaa tatccgccgc cgaccatacc tggactggct ttttcgccgc gccgagaatg
    44641 gctttcatcg aaggaatttg tggggagttg atatcagtgg aaacagcaac aaccgcaggc
    44701 agcggaatgc ttaaggtttc ggtttcatct tccagttcgc gctcaacggt gagggtatct
    44761 gccgtcaggg agataatttt gctgacgccg ttaactgccg gaatattgag gatttcgccc
    44821 accagcagac caacctgctg ggcataaagg tcggaagaac catcgccaca gaggatcaga
    44881 tcaaagcctg ctttctgggc ggctgcagcc agtgcgctcg ccgtttgttg cggcagtgcc
    44941 tgctcgaact ggtcatcaat caccacaatc agttcatccg ggccgcgcga tagcacatct
    45001 ttacgccctt tggcgttggt cagggcttta ccgcccacac ttaaggctgt cacctgcgcc
    45061 tctgctgcct gttgctttag ctggcaagcc gcttcaatag cgttgagatc gtattggctt
    45121 attttggcat cggctttgct gaagtctaat gaaccatcag cattattgac cgcaatatcc
    45181 tgttcatcag gcacgcactt atagcaagta ataatcttca ttgcatctcc agaaatcatg
    45241 aaggaaacgt cgttcatatc ggtggtgtta attacagata tcacgccctt taatttggaa
    45301 ataaaacttt aatcaccaat attgaaaatg tcacacgcag ataaatccaa ctttcaatat
    45361 tgttaagttc ctcaccaata ttgaaaacat ggcgtagcaa aaagaaattt ttaatattgt
    45421 tttatagatc accaatattg aaagtcagat gctatttttt tacccacaaa agaatataac
    45481 aaactgatta ataaccaaaa ttaacgaaaa acgccctcta ttcactcgaa acaaaaatgt
    45541 gacaccaatc acagaataca gcttattgaa tacccattat gagttagcca ttaacgcgtc
    45601 cacgaggtta ataataatta tattaaatgt taacaaaaat aaaacaaacg ggaacgcaaa
    45661 ataaatattc gttttcacag tggaattaac tcatgaagaa tgaaaagaga aaaacgggaa
    45721 tagaaccgaa ggttttcttt ccgccattaa taatcgtcgg catactttgt tggcttacag
    45781 tcagagatct ggatgcggcg aatgtcgtta ttaatgctgt attcagttac gtcaccaatg
    45841 tatggggatg ggcatttgaa tggtatatgg tggtgatgct tttcggttgg ttctggctgg
    45901 tgtttggccc gtatgccaaa aagcgtttag gtaacgaacc gccagaattt agcaccgcca
    45961 gttggatctt tatgatgttc gcctcctgta cgtctgctgc cgtactgttc tggggatcga
    46021 ttgagatcta ctactacatc tccaccccgc cgtttggctt agaaccgaac tcgacaggag
    46081 cgaaagagtt ggggctggct tacagcttgt tccactgggg acctctgccg tgggccactt
    46141 acagcttcct ttcagtcgcc ttcgcttact tcttctttgt ccgcaaaatg gaagtgattc
    46201 gccccagctc gacactggtg ccgctggtag gtgaaaaaca cgccaaaggg ttgttcggta
    46261 ccatcgtcga caacttctat ctcgtcgcct tgatcttcgc gatgggtacc agtctgggcc
    46321 ttgccacgcc gctggtgacc gagtgtatgc aatggttgtt tggcattccg cataccctgc
    46381 aactggacgc tatcatcatt acctgctgga ttatcctcaa cgccatttgc gtcgcttgcg
    46441 gtctgcaaaa aggggtacgt atcgccagtg acgtgcgtag ttacctgagc ttcctgatgc
    46501 tgggttgggt gttcatcgtc agcggtgcca gcttcatcat gaactacttc accgattcgg
    46561 tggggatgtt gctgatgtat ctgccgcgca tgttgttcta taccgatccc atcgctaaag
    46621 gcggcttccc gcagggctgg accgtgttct actgggcatg gtgggtgatt tacgccatcc
    46681 agatgagtat cttcctcgcc cgcatctccc gtggtcgtac tgtgcgtgaa ctgtgcttcg
    46741 gcatggtgat ggggctgaca gcatccacct ggatcctgtg gaccgtgctc ggtagtaaca
    46801 ctctgctgtt gatggataaa aacatcatca acattccaaa tctgatcgaa cagtacggtg
    46861 tggcgcgcgc catcatcgaa acctgggccg ctctgccact cagcaccgcc accatgtggg
    46921 gcttcttcat cctctgcttt attgccaccg ttacgctggt taacgcctgc tcttataccc
    46981 tggcgatgtc tacttgccgc gaagtacgcg atggtgaaga accaccgctg ctggtgcgta
    47041 tcggttggtc aattctggtt ggcattatcg gtattgttct gctggcgctc ggcggcctga
    47101 aaccgattca aaccgccatt atcgccggag gatgcccgct gttcttcgtc aacattatgg
    47161 tgacgctctc ctttattaaa gacgcgaaac agaactggaa agattaatta acccccaaaa
    47221 tatcaagagg ttgaaagatg gattttaatt taaatgatga gcaggaactg tttgtcgccg
    47281 gtatccgcga actgatggcc agcgaaaact gggaggccta ttttgccgag tgcgaccgtg
    47341 acagcgtcta cccggaacgt tttgtcaaag cactggcgga tatgggtatc gacagtctgc
    47401 tgatccctga agagcacggt ggtctggacg cggggtttgt tactctcgcc gccgtgtgga
    47461 tggagctggg acgtctgggg gcaccaacct atgtactgta ccagctgccg ggcgggttca
    47521 ataccttcct gcgcgaaggc acacaagagc agatcgacaa gattatggct ttccgcggca
    47581 ccggtaagca gatgtggaac tcagcgatta ccgaaccggg cgcgggctcc gacgtgggta
    47641 gcctgaaaac gacttatacc cgtagaaatg gtaagattta tcttaatggt agtaagtgtt
    47701 ttattaccag tagcgcctac accccgtaca tcgtggtgat ggcgcgcgac ggggcttctc
    47761 cggacaaacc tgtctacacc gaatggtttg ttgatatgag caaaccgggc atcaaagtga
    47821 ccaaacttga gaagctcggt ctgcgtatgg atagctgctg tgaaatcacc tttgacgatg
    47881 tggaactgga cgagaaagac atgttcggtc gggaaggtaa cggctttaac cgcgtcaaag
    47941 aagagttcga ccatgaacgt ttcctggtag ccctgaccaa ctacggtacg gcgatgtgcg
    48001 cctttgaaga tgcggcgcgc tacgccaacc agcgcgtgca gtttggcgag gctattggtc
    48061 gtttccagtt gattcaggaa aaattcgccc acatggcgat caaattaaac tccatgaaaa
    48121 acatgctgta tgaagcagcg tggaaagcag acaacggcac catcacctct ggcgatgcag
    48181 cgatgtgcaa atacttctgc gccaatgcgg catttgaagt ggtggatagc gcaatgcagg
    48241 tgctgggcgg tgtcgggatt gcgggcaacc accgcatcag ccgcttctgg cgtgacctgc
    48301 gtgtagaccg cgtttccgga ggatctgacg aaatgcagat cctgacgctg ggtcgtgcgg
    48361 tgctgaagca ataccgctaa gtccgtgcgc tgcctgatgc gacgctgacg cgtcttatca
    48421 ggcctacggg agcacgaatg taagccggat aaggcgttta cgccgcatcc ggcagtcatg
    48481 cgctacataa atttttcagg agctaataat ggatcatcta cccatgccga aattcggacc
    48541 gctggccgga ttgcgcgttg tcttctccgg tatcgaaatc gccggaccgt ttgccggaca
    48601 aatgttcgca gaatggggcg cggaagttat ttggatcgag aacgtcgcct gggccgacac
    48661 cattcgcgtt caaccgaact acccgcagct ctcccgccgc aatttgcacg cgctgtcgtt
    48721 aaatattttc aaagatgaag gccgcgaagc gtttctgaaa ttaatggaaa ccaccgatat
    48781 cttcatcgaa gccagtaaag gtccggcctt tgcccgtcgt ggcattaccg atgaagtact
    48841 gtggcagcac aacccgaaac tggttatcgc tcacctgtcc ggttttggtc agtacggcac
    48901 cgaggagtac accaatcttc cggcctataa caccatcgcc caggccttca gtggttacct
    48961 gattcagaac ggtgatgttg accagccaat gcctgccttc ccgtataccg ccgattactt
    49021 ttctggcctg accgccacca cggcggcgct ggcagcactg cataaagtgc gtgaaaccgg
    49081 taaaggcgaa agtatcgaca tcgccatgta tgaagtgatg ctgcgtatgg gccagtactt
    49141 catgatggat tacttcaacg gcggcgaaat gtgcccgcgc atgagcaaag gtaaagatcc
    49201 ctactacgcc ggttgcggtc tgtataaatg tgccgacggc tacatcgtga tggaactggt
    49261 gggcattacc caaattgaag agtgctttaa agatattggc ctcgcacatc tactcggtac
    49321 gccggaaatc ccggaaggca ctcagcttat ccaccgtatc gaatgccctt acggcccact
    49381 ggtagaagaa aaactcgatg cctggctggc ggcacatacc atcgcagaag ttaaagaacg
    49441 ctttgccgaa ctgaatatcg cctgtgccaa agtactgacc gtaccggaac tggaaagcaa
    49501 tccacagtat gttgcccgtg aatcaatcac tcagtggcaa acgatggatg gtcgcacctg
    49561 caaagggccg aacatcatgc cgaaattcaa aaataacccc ggacaaatct ggcgcggaat
    49621 gccctcacat ggcatggaca cggctgccat tttgaaaaat atcggctaca gcgaaaacga
    49681 cattcaggag ttggtcagca aaggtctggc caaagttgag gactaaacgc gtggcgtcag
    49741 tcggcttgcc tgactagcgc ccgcccaata acaaacgaac aaaaatggat agaggtgcaa
    49801 tggatatcat tggcggacaa catctacgtc aaatgtggga cgatctggcg gacgtttacg
    49861 gtcataaaac ggcgctgatt tgtgaatcca gcggcggagt cgttaaccgg tatagttatc
    49921 ttgagttaaa tcaggagatt aaccgcacgg caaacctgtt ttatacgctg gggattcgca
    49981 aaggcaacaa ggttgcacta catctcgaca actgcccgga atttatcttt tgctggttcg
    50041 ggctggcaaa aattggcgcg attatggtgc cgattaacgc ccgcctgtta cgcgaggaaa
    50101 gcgagtggat cttgcaaaat agccaggcgt gcctgctggt gaccagtgcg caattttatc
    50161 ctatgtatca acagattcag caggaagatg ccactcaatt gcggcacatt tgcctgacag
    50221 atgtggcact tcccgctgat gatggagtga gttcatttac tcaactgaaa aatcaacaac
    50281 ctgccacctt gtgctatgca ccgccgctat cgactgacga tacggcggaa attctcttca
    50341 cctccggcac cacctcccga ccgaaaggtg tggtgattac ccattacaac ctgcgcttcg
    50401 ctggatatta ctccgcctgg cagtgtgcac tgcgtgacga tgacgtctac atgacggtaa
    50461 tgcctgcgtt tcatatcgat tgccagtgta ctgcggcgat ggcggcgttt tctgccgggg
    50521 ccacctttgt gctggtcgag aaatacagcg cccgcgcctt ctggggacag gcgcagaagt
    50581 accgcgccac cattaccgaa tgtattccga tgatgatccg tacgttgatg gtgcagccgc
    50641 cttcagcgaa cgatcggcaa caccgcctgc gggaagtgat gttttatctc aacttgtcgg
    50701 agcaggaaaa agacgcattt tgtgaacgct tcggcgttcg cttgctgacg tcttatggga
    50761 tgacggaaac cattgtgggc attatcggcg atcgccctag cgataaacga cgctggccgt
    50821 cgattggtcg ggcggggttt tgctacgaag cggagatccg cgacgatcac aatcgcccgc
    50881 tcccggcagg tgagatcggt gaaatctgta ttaaaggcgt accagggaaa accatcttca
    50941 aagagtattt tctcaacccg aaagccactg cgaaagtgct ggaagccgac ggctggctac
    51001 ataccggcga taccggatac cgcgacgaag aaggcttttt ttatttcgtc gatcgccgct
    51061 gcaatatgat caaacgcggt ggcgagaatg tctcctgcgt ggagctggaa aatatcatcg
    51121 ccacccatcc gaaaattcag gacatcgtgg ttgtgggtat taaagattcg attcgcgatg
    51181 aagccatcaa agcatttgtg gtgctgaatg aaggtgaaac attgagcgaa gaggaatttt
    51241 ttcgcttctg cgaacaaaat atggcgaaat ttaaagtgcc ctcttatctg gagatcagaa
    51301 aagatctgcc acgtaattgc tcggggaaaa taattagaaa gaatctgaaa taaatgaaac
    51361 gtcagggaac taccctgcca gctaataatc acgccctaaa acaatatgca ttttttgcag
    51421 ggatgttgtc gtccctaaaa aagcaaaaat ggagaaaagg aatgagtgaa tcattacatc
    51481 tgacccgcaa tggatcaatt ctggaaatta cccttgatcg cccaaaagcg aatgctattg
    51541 atgcaaaaac cagctttgaa atgggcgaag tatttctaaa tttccgtgac gatccgcaat
    51601 tacgtgtcgc cattattacc ggtgccggag agaaattctt ttccgcgggc tgggatttaa
    51661 aagcggcagc agaaggcgaa gcaccggatg ctgactttgg cccgggtggt tttgcgggat
    51721 taaccgaaat tttcaatctc gacaaaccag ttatcgcagc tgtgaacggc tacgcctttg
    51781 gcggcggttt cgaactggcg ctggcggcag attttattgt ttgtgccgat aacgccagct
    51841 tcgccctgcc ggaagccaaa ctgggcatcg ttcctgacag cggcggtgtg ctgcgtctgc
    51901 cgaagatcct gccgcctacc atcgtcaatg aaatggtgat gaccggcaga cgaatgggcg
    51961 cagaagaggc gctgcgttgg gggatagtca accgcgtggt tagccaggcg gaactgatgg
    52021 ataacgcccg cgaactggct cagcagctgg ttaacagcgc cccgctggcg attgcggcgc
    52081 tgaaagagat ctaccgcacc accagcgaaa tgccggtaga agaagcgtat cgctatattc
    52141 gcagcggcgt gttgaaacac tatccatcgg ttctgcattc ggaagatgcc attgaagggc
    52201 cgctggcgtt tgccgagaag cgcgatccgg tgtggaaagg acgttaacaa ccgtgagcta
    52261 ttacgccttt gaggggttaa ttccggtggt tcacccgacg gcgtttgtcc atcccagtgc
    52321 cgtcttgatt ggcgatgtga ttgtgggagc cggtgtctac atcggcccac tcgcctcact
    52381 gcgtggtgac tacgggcggt tgatcgtgca agcgggagcc aatattcagg atggctgcat
    52441 tatgcatggc tactgcgaca ctgacaccat cgttggggaa aacggccata tcgggcatgg
    52501 agcgatcctg catggttgtg tgattggtcg cgatgcattg gtcgggatga acagcgtaat
    52561 tatggatggc gcggtcattg gcgaagagag cattgttgcc gcaatgagct ttgtcaaagc
    52621 aggctttagt ggcgagaaac gccagttgtt gatgggtacg ccagcccgcg ctgtacgcag
    52681 tgttagtgac gacgagttac actggaaacg gttgaatacc aaagagtatc aggatcttgt
    52741 cgggcgctgt catgcatcgt tacatgaaac gcagccactg aggcaaatgg aggaaaatcg
    52801 cccccgtttg caggggacga cggatgtgac gcctaaacgg tgatcttgcc ggatgcgacc
    52861 tggttgctgg ataagatgct acaacatcgt atctggcaag caggacgccc ggtatcaacg
    52921 agataaaatt aacgacgcat actctttgac aacatcatct gccacttctg ctcgcggtta
    52981 agttccgtga gcggaacggc aggaatacgc ggtgtctttc ttacgccgac taatttttcc
    53041 cgactgttat tgcgcaggcg cgcgtaaatt tgctcatcga tatcgaccac tttaaccagt
    53101 cgctgacact ggcatccccg cccttccagc ttattaggga tcatcttaac ttcgcagctt
    53161 atttctgtga cttccgacag aatataagtc agggtattaa ccgccttgct gtgttcaata
    53221 tcgaaatgaa tagagatctc tcttgctatc acccaccgat tttcagccat catccattcg
    53281 gcgattaaca agtagagtgg tttttcaaca tatccttcac acatacgaac tccagtttca
    53341 caaattaaaa gtctggctat gactcgtttt gaagcaatta aacaaggcca tattaaaatt
    53401 gtggatatat cgatagtatg caacttcacc gtcgataagt gtgagctaaa tcctgcttat
    53461 gtcatcaaaa atatcgattc cccaaaagat ttacttaatg ggcaaaaaaa aacggtcctc
    53521 atcagagaac cgtattagtt attcgatcaa attagcggat gaaaaatatc cgccacgaca
    53581 cgctattatt tgatctgtgc gtgcatttcc tgcaccgaaa ttactttttc agtcgcatcg
    53641 gcattcagcg ccatcgcggt agcgaaacca ccgttcaggg tggtgtcata atgcacttta
    53701 tattgcagcg cactgcgacg gatcacgcgg gagtcttcaa tcgcacgacg gcctgaggtg
    53761 gtgttgatga tgtaggtata ttcgccattc ttgatacggt cctgaatgtg cggacggcct
    53821 tcatgcacct tgtttaccag acgcggattg atacccgctt cgcccagcac aatcgccgtg
    53881 ccgtgggtcg catccagctc gaagccctgt ttcagcagtt ttgccgccag gtccaccacg
    53941 cgttctttat cgccttcgcg cacggaaagc agcgcacgac cgtgtttctt catggtggag
    54001 ttgctgccca gctgcgcttt ggcgaacgct tcagcgaagg tgcggcccac gcccatgact
    54061 tccccggtag agcgcatttc tggccctaac agcgggtcaa cgcctgggaa tttattgaac
    54121 ggcagcacca cttctttcac cgagtagtac ggcggaataa cttctttggt tacgccctgc
    54181 tcagccagcg atttgccagc catcacacgc gccgccactt ttgccagcgg tacgccggtg
    54241 gctttggaga cgaacggaac agtacgcgcc gcacgcgggt ttacttcaat caggtaaact
    54301 tcgttgtttt tcaccgcaaa ctgcacgttc atcagaccgc gcacctgcaa ttcgaaggcc
    54361 agtttctgca cctgctggcg catcacatcc tgaatttcct gacttaaggt gtacgctggc
    54421 agcgaacatg cggagtcacc ggagtgcaca cccgcctgct cgatgtgctc catgatgccg
    54481 ccaatcagca ccatttcgcc gtcgcagatg gcatccacgt caacttctac tgcgtcatca
    54541 aggaaatgat ccagcaacac tggcgcatcg ttagacacgc taaccgccgt ctggaagtag
    54601 cgacgcaggt cagcttcgtc atagacgatt tccatcgccc gaccgccgag aacgtaagac
    54661 ggacgcacca ccagcgggta gccaatctct tttgccttct caactgccat ttcaatagca
    54721 gtaacggtgg cgttcgccgg ttgtttcagt ttcagacggt caaccgcatg ctggaagcgt
    54781 tcacggtctt ccgcacggtc gatagcatcc gggctggtgc cgataaccgg tacgccagcg
    54841 gcttccagtg cacgcgccag tttcagcggg gtctgaccgc cgtactgaac gataacgcct
    54901 ttcggcttct caatacgcac gatttccagc acatcttcca gagttaccgg ctcgaagtag
    54961 aggcgatcgg aagtgtcgta gtcggtagag acggtttccg ggttacagtt aaccataatg
    55021 gtttcgtaac cgtcttcacg cagcgccagc gaggcgtgta cgcagcagta gtcgaattcg
    55081 ataccctgac cgatacggtt tggaccgccg ccaagcacca tgattttttc acggtcggta
    55141 gacggattcg cttcgcactc ttcttcataa gtggagtaca tgtaagcggt gtcggtggca
    55201 aactctgccg cacaggtatc cacgcgcttg tagaccgggt gcaggtcata ttggtcacgc
    55261 agcttacgga tttccgcttc gcgtacgccc gccagttttg ccaagcgcgc atcggcaaag
    55321 cctttgcgtt tcagctggcg caggaattca gcgttcaggc cagtgatgcc cacttccgcc
    55381 actttctctt ccagacgcac cagttcttca atctgtacca ggaaccagcg gtcaatgtta
    55441 gtcaggttga agacgccgtc cacagacagg cccgcgcgga aagcatcggc gatgtaccag
    55501 atacgctctg cgcctgcgtc tttcagttcg cgacggattt tggttaacgc ttccgggtca
    55561 tccaggctca ctttcgggtc gaagccagtt gcgcccactt ccaggccgcg cagcgctttt
    55621 tgcagggatt cctgctgcgt gcgaccaatc gccatcactt cgccaaccga tttcatctga
    55681 gtggtcagac ggtcgttagc accggcgaat ttttcgaagt tgaagcgagg aattttagta
    55741 accacatagt cgatggacgg ctcgaaggag gccggagtac gtccgccagt gatgtcgttc
    55801 atcagttcgt ctagggtgta acccaccgcc agtttcgccg ccactttagc aatcgggaaa
    55861 ccggtcgctt tcgacgccag cgccgaagaa cgggacacgc gtgggttcat ttcgataaca
    55921 atcaggcgac cgtttttcgg gttcaccgca aactggacat tggaaccacc ggtttcaacg
    55981 ccgatttcac gcagcaccgc catcgaggcg ttacgcatga tttgatattc tttgtcggtc
    56041 agcgtttggg ctggcgcgac agtgatggag tcaccggtgt ggatgcccat cgcatcaaag
    56101 ttttcgatag agcagacgat gatgcagttg tcgtttttat cacgcaccac ttccatctcg
    56161 tactctttcc agccgatcag cgactcatca atcagcaact ctttggtcgg agagagatcc
    56221 agaccgcggg cgcaaatttc ttcaaactct tcacggttat aagcgatacc gccgccgcta
    56281 ccgcccatgg taaaggatgg gcgaataatg cacgggaagc ccacgtcagc ggcaacagcc
    56341 agcgcttctt ccatcgtgtg tgcgataccg gaacgcgcgg tttccagacc aattttcttc
    56401 atcgctacgt cgaaacgacg gcggtcttct gctttatcaa tcgcatcggc agtggcacca
    56461 atcatggtga caccgaactc ttccaacacg ccctgacgtt ccagctccag cgcgcagttc
    56521 agcgccgtct gaccgcccat ggttggcagc accgcgtccg ggcgctcttt ttcaataatc
    56581 ttgcgtacca cttcccagtg aatcggctcg atgtaggtcg catcggccat ttccgggtcg
    56641 gtcatgatgg tcgccgggtt ggagttcacc agaatgacgc ggtaaccctc ttcgcgcagg
    56701 gctttacatg cttgcgcgcc agagtagtca aactcacacg cctgaccgat aacaatcggg
    56761 cccgcaccca gaatcaggat actttttata tctgtacgtt ttggcatggc tcttttactc
    56821 ctgattactt agcggtttta cggtactgct caattaactt gataaagtgg tcgaacaacg
    56881 gcgcggcgtc gtgtggacca gggctggctt cagggtgacc ctggaagctg aacgccggct
    56941 tatcgatgcg atgaatgccc tgtaacgtac cgtcgaacag ggatttatgc gtgacacgca
    57001 ggtttgcagg taatgttgct tcatccaccg caaaaccgtg gttctgggcg gtgatcatca
    57061 ccacgttttt ctccacatct ttaaccggat ggttgccgcc gtggtgacca aatttcattt
    57121 tgacagtctt cgcaccgctc gccagcgcca gcagctgatg accgagacag atgccgaata
    57181 ccggaatatc ggtttcgagg aacttctgga tggcggtaat ggcgtaatcg cacggggccg
    57241 ggtcgccagg accgttggag aggaagatgc cgtctggatt cattttcagc acatcttccg
    57301 cagaagtttg cgccggaacg atggtcaggc gacagcctct atccaccagc atccgcagga
    57361 tgttgcgctt ggcaccaaaa tcataagcca cgacgtggaa cggcagctcg tcttcttttt
    57421 tcgcttctgg caggccgccg gtcaacgtcc agctcccttg tgtccagcta taggcttctg
    57481 cggtggtcac ttcttttgcc agatccatgc cgttcagacc tgggaacgcg cgggcttttt
    57541 ctaacgccag cgccgcatcc gggttatcgc ctgcgataat gcagccattc tgtgcgcctt
    57601 tctcgcgcag taaacgcgtc agcttacggg tatcgatatc ggcaatcgcc acgatgttat
    57661 ggcgcttcag gtaagaagag aggtcttcgg tattacggaa gttgctggca atcagcggca
    57721 ggtcgcgaat caccagacct tgtgcatgta cctgagaaga ttcttcatcg gcgtcattag
    57781 tgccgacatt gccaatatgg ggataagtaa gagtaacgat ttgacgagaa taggaaggat
    57841 cagtgaggat ttcttgataa ccggtcattg aagtattgaa aacgacttcc ccaaccgccg
    57901 aacctgttgc ccctatggcc cgaccgtgaa actgggttcc gtcttccaga accaatagcg
    57961 ctgacttaat caaaacaccc tccagagaat actcactcac tttatttgca tattaattca
    58021 cagtgatgac gtaaatcaat gcaaatctgc ttaccgacga atttctggca aacggcggca
    58081 ttctggagat atgggcgcta aaagtcaact taatggcagg attttttata ttattttgtg
    58141 gctcatgcat gttttacagg cattaagcga caaaaacatc atctaacctg ttctggaaaa
    58201 cgcttgcgca gcaagaagat tgcatgaaat aaaaacgagc aggaaaaagt ggaccaaatg
    58261 gtcaaaattt acattaaaac aacaaaaata gaagacgaaa catgtaattt ttggcagtta
    58321 tagaaaattt aaaagcataa caccaaaaat aaaagggcta aaaattaaag ccctttataa
    58381 ttaataaatt aaatgtgtta cttttgcatc ataataaaca ttttgtggtt acaaattatt
    58441 gagatcaagc acatctcgca tatcaaaaag accactttcc ttaccactca accacaaagc
    58501 cgatcttacc gcaccgttag caaatgtcat gcggctggac gccttatggg tgatctccag
    58561 acgctcgcca atatcggcaa acatcgcggt atgttcgcca acgatgtcac ctgcacgcac
    58621 ggtagcaaaa ccaatggtac ctggcacacg ttcaccggta tggccttcac gactgtagac
    58681 cgcgcaatct ttcagatctt tatcaagggc gtgggcgatc gcctctccca ttgccagtgc
    58741 ggtgcctgac ggcgcatcaa ctttatgtct atgatgtgct tcaataattt cgatatcggt
    58801 gtagtcaccc atcactttgg ctgctttctc cagcagctta agcatgacgt taacgccaac
    58861 gctaaaattg gcggcaaaga caatcgcaat atcggcggcg gcgtcacgaa ttgcctgttt
    58921 accggcttcg tcaaaccccg tagtgccgat caccatccct ttgccatgct gccgacaaaa
    58981 agcgagatgg ttcagcgtac cttccggacg ggtaaaatcg ataaacacat caaaatcatc
    59041 ttttatcgca tcaagactgc tttgcacggt aacgcctgtt ttccctgcac cagccagctc
    59101 accggcgtcg ctgcccagta aagaagatcc ttcgcgctcc agcgccgcgc ccagctgcac
    59161 gccttctaat gccagtgccg cctgaatcaa ctggcggccc atacgccccc cggctcccgc
    59221 gatggcaacg cggatgtttg catcatgcat agctattctc ttttgttaat ttgcatagac
    59281 cgttttcaga gtaaccagcg catcgctacg ccgccagcca aaaacgctga taattagaaa
    59341 ttaatgataa acagggaggg atatcagtga aaggcatgag tcgatgaatg actgcatgcc
    59401 gataacatgt gacaggttac ggcaccagag ccagcacctc agccacccac tgttggaagc
    59461 cttttacatt cagatccaat gccacctgta cattggctgg tttgcccagg caaccgtcga
    59521 tatcaaccac cgtcgtgcct gaggtaaatt cgccttgagt ttctaccgcg acaaaacacg
    59581 gcttgagggt gaataagtcc gggcgcacca gccaggcgat ggcgcagaga tcgtgcattc
    59641 gcagaccgct ttgcatactg ccgctacggt agtggctaaa cagggcgtgg agcatttttc
    59701 cggtgcgatt caactccggt aaagtagcga gatagtcagg ggttaatatt gcctgattgg
    59761 tgacatccag accgcacatg acaatttcga tgccgctacg gaagacacag gcagccgctt
    59821 ctggatcggc agcgatatta aactcggcat ttggtgtaca gttgccgcga ccagcagaac
    59881 cgcccatgat caccagacga cgaatatacg gtttacattc cgggcattgt aaaagtaaca
    59941 gcgcaatatt ggttaacggg ccgatggcca ccagggtaac gggctctggt gcacgcatca
    60001 gggcatcccg aatcgccaga aacgccggta tcccgagtgg ctttcggttg tgctcaacaa
    60061 agtcgtagcc agccattccc gattcgccgt gcacagatgc tgcatcacgc ggtgcgcgta
    60121 ccagcggcac agcggctccc tgggcgagcg gaatctccgc attccagaaa tgcagcagtt
    60181 gcagggcatt gcgggtagtt ttctcaaccg agacattacc cgcgacggtg gtcatcagtt
    60241 gcaggtcgag ttcgggggca aaaatcgcgg cggcaatggc gacggcatcg tcaatgccgg
    60301 ggtcagtatc gaggaagata ggtaaacgca tgttttcttc ataaaaaatg ccggtaacaa
    60361 gaccggcatt ttcgcataac ttaggctgct aatgacttaa tcgacttcac gaatatcgac
    60421 acgcagctct ttcggcactt cgaaaacaat attttcttca cggccttcca gcggaatggc
    60481 ttcaccacca cccaactgct gcaaacgtgc caccacattc tgcaccagaa tatccggagc
    60541 cgatgcgccc gcagtcacgc cgacgcattt aacctctttc acccactctt cctggatatc
    60601 tttcgcatcg tcaatcaaaa acgcgtgttt gcccatacgc tgggccagct ccgccagacg
    60661 gttggagttg gaggagtttt tcgaaccgac caccaacaca acttccgcct gttctgccag
    60721 ggcgcgtacc gcttcctgac ggttagtcgt ggcgtagcag atgtcatctt tgcgcggacc
    60781 gacaattttc gggaagcgtt tacgcagcgc gtcgatcaca tcagacgtgt catccaccga
    60841 cagcgtggtt tgggtcataa aggagagctt ctcttcgttt ttgaccgtca gtttccacac
    60901 atcgtctggc gattcgacca gatacattcc cccttcgggg ttgctgtact gccccatcgt
    60961 cccttccact tccgggtgcc cggcgtggcc gatgagaata gattcttcgc cacggcgact
    61021 ggcgcgggca acttccatat gcactttggt caccagcgga caggtggcat caaacaccgt
    61081 caaatcgcgg cttttcgctt cgttacgaac cgcctgagaa acaccgtgtg cggagaaaat
    61141 caggatcgcg ccgtccggca cttcgctaat ctgctcaata aagatagccc cacgctcgcg
    61201 caagctatcg accacgtagc ggttatgcac cacttcgtga cggacatata tcggtgcgcc
    61261 gtaaatggcc agcgcgtttt caacaatgct gatagcgcgg tctaccccgg cacaaaaacc
    61321 acgcgggttg gccaacagga tctgcatgtt acgcctccag tgccggatcg atttccagca
    61381 cttcaatatc aaaatgaacg gtctgcccgg ccagcggatg gttgaaatca acggtaatgg
    61441 agtcgccgtt aatttcgcgg atcacaccag gcatctcact gccatccatt gcggtaaaaa
    61501 gcatgattgc gccaatttct ggctcgcctg catccataaa ttcacggcgg gagaagtact
    61561 gaatcaggtc cggtgacggc acgccaaacg ccgcatctgg ctccagcgag aaggtggttt
    61621 tgtcgcccac tttcagcccc agcaggtgtt gctccagccc ttcagaaaga gaagcatcgc
    61681 ccaggcggaa cagcgccggt ttaccgttgt tgcgagtaga ctcggcggtg gtgccatcgt
    61741 cgagttttag cgtgaagtgc accaggacgg cgctattgct ctgtacagat tcagacatgc
    61801 aggtttgctc ttatttttga ggatttttgc cggatgcggc ctacgatttc gtagcaatct
    61861 gtaggccgca tccggcaggg tttattattg ttttttcgct ttggaaggta aaaacccttc
    61921 cagcacgatc agtgccgcac cgacacaaat ggcagtatcg gcaaggttga aggtggcgaa
    61981 gtgccagtcg ccgacgtaga agtcgatcat atcgacaacg aagccgtgcc acaggcggtc
    62041 gaacaggttg cccagcgcgc cgccaataat cagcgcgtaa gcgatattgt ttagcttctg
    62101 cgtggccttc gagcgataca tcatcactgc caggagcacg ctaataccaa tcgcaatacc
    62161 ggcaaagaac caacgctgcc agccgccgct atcggcaagg aaactaaacg ccgcgccata
    62221 gttacgcgca taatgcagat taagcgacgg gaacagcggg accgtatccc ccagagcaaa
    62281 gttctggagg atcaggtatt tgctgcccag atcgataatc agcacgacta ccaccagcca
    62341 cagccagcgt agccctgttg aacagatcga ttgactcatc aggcaaactt acgtttttca
    62401 ccgtcaccgg cgacgttgct gacacagcgg ccgcagattt ctgcgtgttc cgccaccttg
    62461 ccgacatcct gggtgtagtg ccagcagcgt gggcacttct caccttcggc tttactcaac
    62521 gcgactttca gccctttgag tacttcgctc tgctgagcat cagcaggtgc gtcgttatag
    62581 tctgcaacgg tagcgccgga ggtcaacagg acaaatcgta attcatcgcc cagcgcggtc
    62641 agtttcgccg ccagttccgg ttctgcatac aaggttactg ccgcttccag cgagccaccc
    62701 actttcttgt cggcacgcgc ttgctcaatg actttgttca cttcgccacg cactttcaac
    62761 agctcgtccc agaacgcatc gttcattgct tcactgtctg ccagaccaaa caggccttcg
    62821 taccactcac cggtgaagac gtatttttca cgttcgcccg gcagatagcc ccacacttca
    62881 tcagcggtga aggagaggat tggtgccatc cagcgcacca gcgcttcagc gatgtgatac
    62941 agcgcagtct ggcagctacg acgcgccacg ctatccgctt tggcggtgta ctgacggtct
    63001 ttgatgatgt cgaggtagaa ggaacccatc tcaacggagc agaagcgcat cagacgctgt
    63061 accacttcgt ggaaatcgta tgcttcgtac gccttgagga tgtcttcctg tgccgctttc
    63121 gcacaaccta cggcccagcg atccagtacc accatctctt ccggtttcac catatctttt
    63181 gctggatcaa aaccattcag gtttgccagc aggaagcgcg cggtgttacg gatacgacga
    63241 tagctatcgg cagcacgttt caggatctcg tcagaaacgg ccatttcacc ggtgtagtcg
    63301 gttgatgcca cccacagacg cagaatatcc gcgcccagtt tgttcatcac atcctgcggc
    63361 gaaacggtgt tgccgatgga tttagacatc ttgcgaccct gaccatccac ggtaaagccg
    63421 tgggtcagta cctgacgata cggcgctttg cctttcatcg cggtggagat cattagggaa
    63481 gacatgaacc aaccacggtg ctggtcagaa ccttccagat acatgtccgc tgcgtgaccg
    63541 gcaaattccg gacgcacgtc aacaacagaa gagtgggtag atccggagtc aaaccatacg
    63601 tcgagagtat ccggcacttt cacatactgg tcagcttcgt cgccgaggat ctctttcgca
    63661 tcgagatccc accacgcctg gatgccgtca acttcaacgc gtttagccac ttcttccatc
    63721 agttcgaggg tacgcggatg cagctcttcc gtgtctttgt gcacgaacag tgacatcggt
    63781 acgccccagg tgcgctgacg ggagatacac cagtcaggac ggttagcaac catcgactcg
    63841 atacgcgcct ggccccagtc cgggagccac tgcacgcctt tgatctcttt cagtgactgc
    63901 gcacgcagac ctttctgatc catgctgacg aaccactgcg gtgtcgcgcg gaagatgatc
    63961 ggcgttttgt gacgccagca gcacggatag ctgtgctgca ttttctcaac gtgcagcagc
    64021 gcgccttttt cctgcagcag cgcaacgacg atgtcgttcg ctttgaagac gttcacgcca
    64081 tccagcgtcg gataagtgcc cggcagataa gtgccgtccg ggccaaccgg gttagcggtt
    64141 tccaggccgt atttctgacc gatcacatag tcgtccgggc cgtggccagg cgcggtgtga
    64201 acggcaccgg taccggcatc cagggtaacg tgatcgccga ggattgccgg aacgtcgaag
    64261 cccataaacg gatgggtaaa gcgcagcagc tcaagctctg caccttttac cgtgccgaga
    64321 atggtgtaat cggtcacgcc gatacgctgc attacgcttt caaccagatc tttcgccaga
    64381 atcacggcct gaccgtcgat ctgcaccagc gcatagtcga aatcaggtgc aatagagatt
    64441 gcgcggttcg caggcagagt ccacggcgta gtggtccaga tcaccagcga gattgggccg
    64501 ttaacgttgc ttacgccaaa tttcgctttc agcgcatcct gatcgaccgc ctggaaagcg
    64561 acgtcaatgg acggagaagt tttgtcgtaa tactcaactt ccgcttctgc cagtgcagaa
    64621 cggcagtcta cgcaccagtg caccggcttc gcgcctttgt gcaggtgacc attgccgatg
    64681 attttgccca gcgcgcggat gatgttggct tcagttttga agtccatagt cagatacggg
    64741 tgcgaccagt cgcccagcac gcccagacgg ataaagtctt tgcgttgacc gtcaacctgg
    64801 gtcgccgcgt attcgcggca cttggcgcgg aactcggcgg cggtgaattt ctcacccggc
    64861 ttaccgtatt cttgctctac tttcagctcg atcggcagac cgtggcagtc ccagccaggc
    64921 acatacggcg agtcataacc ggaaagccct ttggacttca cgataatgtc tttcagaatc
    64981 ttgttaaccg agtgaccaat atgaatgctg ccattcgcat aaggagggcc atcatgcaga
    65041 atgaaggttt ttttgccttt tttagccgca cggatgatgc cgtacagatc atcatcagtc
    65101 caacgcgcca gcattcccgg ttcgcgcttg gcgagatcgc cacgcatcgg gaaccctgtt
    65161 tccggcaaat tcagggttga tttatagtca ctcatcagat tctcggttcc gtgtttcggt
    65221 ttgattacat aacaggctta agccggtttt gttagcccaa aaaattcgcg ggcggttaat
    65281 tcatcacgcg caatctgcgc tttcagttcg tccagcgacg caaatcgctg ctcattgcgt
    65341 atttttttac gcagcactac ttgtatatgg cgaccgtaaa ggtccattgc aacatctaac
    65401 aaatgcactt ccagctgctg gcgaataccg gcaaccgttg ggcgtgttcc gatgtttgcc
    65461 acgccgggta acggcttttc accgaggccc agcacttcta ccgcataaac ccctttcacc
    65521 ggggaaacct gacggcgtag cggtacattc gccgtcggga aacctatagt gcgccctaat
    65581 tcatcaccgt ggactacacg cccggagatg gcaaacgggt gccccagtaa actttctgcc
    65641 agagccagat tgtcatcagc cagcgcctga cgcacggcgg tgctgctgat acgcacacca
    65701 ccttcgcaaa aagtttgcgt actggtgata tcgaagccgt attccatgcc agctttctgt
    65761 aataacaaga aatcgccttc acgaccagcg ccaaagcgga aatcatcacc tacggcaaga
    65821 aattttacgc gcaaatgctt caccaaaaga tcgctgatga aattttgcgc ggttaacgcc
    65881 gcaaaacgcc tgtcgaaacg cacgcacagc acgtaatcaa cgccacactc tgcaaggtaa
    65941 cgcagttttt cccgcagccg ggtcagtctt gccggggctt tatcggtagc aaacagttcc
    66001 agtggttgag gttcaaaaag catcaccatc accggtaagt tgcgcttgcg cccttcttcc
    66061 tgcaagccct gtaacagcgc gcgatgaccg cgatgcacgc cgtcgaaatt accaatagtc
    66121 agcacacacc cttcttgcgg ggcctggctg agattatgta tgccgcgtat cagcttcatg
    66181 tctggctcaa aacagtgaaa atcgtccgag tataccttgt acagcggtca aggttaaccg
    66241 gcgattgagt accgaaatca gaaagaagtg gcttttcatt gccatggagc aaatcacggg
    66301 aagaaactga ccgcctgctg caatttttat cgcggaaaag ctgtattcac accccgcaag
    66361 ctggtagaat cctgcgccat cactacgtaa cgagtgccgg cacattaacg gcgcttattt
    66421 gcacaaatcc attgacaaaa gaaggctaaa agggcatatt cctcggcctt tgaattgtcc
    66481 atatagaaca catttgggag ttggaccttg gctaatatca aatcagctaa gaagcgcgcc
    66541 attcagtctg aaaaggctcg taagcacaac gcaagccgtc gctctatgat gcgtactttc
    66601 atcaagaaag tatacgcagc tatcgaagct ggcgacaaag ctgctgcaca gaaagcattt
    66661 aacgaaatgc aaccgatcgt ggaccgtcag gctgctaaag gtctgatcca caaaaacaaa
    66721 gctgcacgtc ataaggctaa cctgactgca cagatcaaca aactggctta atcgccaatt
    66781 tgctgaagct ttgtgaaaaa gcccgcgcaa gcgggttttt ttatgcctgc tgcttttgtg
    66841 atgcgtgaga gaacacactg ccggttgcca gatagtcaaa agagacatgc cgttgtacta
    66901 tctgaactct tcgtggctta agaatcgtag aaccatcggc tgatacattc tgaacaagaa
    66961 tatctactca atctccagac tcccccacaa agaatatgga tattgtgata cacattgagg
    67021 tattaaactt aagaccactc tatttttgaa ttaaattcct atatacttta accattcata
    67081 aatatctcct cattagacct
//
LOCUS       DAEEUE010000008        66140 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.008, whole genome shotgun sequence.
ACCESSION   DAEEUE010000008 DAEEUE010000000
VERSION     DAEEUE010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66140
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.008"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..88
                     /locus_tag="KLK84_000551"
     CDS             <1..88
                     /locus_tag="KLK84_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE2008011.1"
                     /translation="VRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            complement(236..715)
                     /gene="folK"
                     /locus_tag="KLK84_000552"
     CDS             complement(236..715)
                     /gene="folK"
                     /locus_tag="KLK84_000552"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBE2008012.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(712..2130)
                     /gene="pcnB"
                     /locus_tag="KLK84_000553"
     CDS             complement(712..2130)
                     /gene="pcnB"
                     /locus_tag="KLK84_000553"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HBE2008013.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2169..3095)
                     /gene="gluQRS"
                     /locus_tag="KLK84_000554"
     CDS             complement(2169..3095)
                     /gene="gluQRS"
                     /locus_tag="KLK84_000554"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HBE2008014.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3132..3587)
                     /gene="dksA"
                     /locus_tag="KLK84_000555"
     CDS             complement(3132..3587)
                     /gene="dksA"
                     /locus_tag="KLK84_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HBE2008015.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3765..4469)
                     /gene="sfsA"
                     /locus_tag="KLK84_000556"
     CDS             complement(3765..4469)
                     /gene="sfsA"
                     /locus_tag="KLK84_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBE2008016.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4484..5014)
                     /gene="thpR"
                     /locus_tag="KLK84_000557"
     CDS             complement(4484..5014)
                     /gene="thpR"
                     /locus_tag="KLK84_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HBE2008017.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            5088..7517
                     /gene="hrpB"
                     /locus_tag="KLK84_000558"
     CDS             5088..7517
                     /gene="hrpB"
                     /locus_tag="KLK84_000558"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HBE2008018.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7713..10247
                     /gene="mrcB"
                     /locus_tag="KLK84_000559"
     CDS             7713..10247
                     /gene="mrcB"
                     /locus_tag="KLK84_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HBE2008019.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10467..12710
                     /gene="fhuA"
                     /locus_tag="KLK84_000560"
     CDS             10467..12710
                     /gene="fhuA"
                     /locus_tag="KLK84_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HBE2008020.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12761..13558
                     /gene="fhuC"
                     /locus_tag="KLK84_000561"
     CDS             12761..13558
                     /gene="fhuC"
                     /locus_tag="KLK84_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HBE2008021.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13558..14448
                     /gene="fhuD"
                     /locus_tag="KLK84_000562"
     CDS             13558..14448
                     /gene="fhuD"
                     /locus_tag="KLK84_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HBE2008022.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14445..16427
                     /gene="fhuB"
                     /locus_tag="KLK84_000563"
     CDS             14445..16427
                     /gene="fhuB"
                     /locus_tag="KLK84_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HBE2008023.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16462..17742)
                     /gene="hemL"
                     /locus_tag="KLK84_000564"
     CDS             complement(16462..17742)
                     /gene="hemL"
                     /locus_tag="KLK84_000564"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HBE2008024.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17967..19388
                     /gene="clcA"
                     /locus_tag="KLK84_000565"
     CDS             17967..19388
                     /gene="clcA"
                     /locus_tag="KLK84_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HBE2008025.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19412..19477
                     /locus_tag="KLK84_000566"
     CDS             19412..19477
                     /locus_tag="KLK84_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2008026.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19470..19814
                     /gene="erpA"
                     /locus_tag="KLK84_000567"
     CDS             19470..19814
                     /gene="erpA"
                     /locus_tag="KLK84_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HBE2008027.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19861..20484)
                     /locus_tag="KLK84_000568"
     CDS             complement(19861..20484)
                     /locus_tag="KLK84_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HBE2008028.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20522..21322)
                     /gene="btuF"
                     /locus_tag="KLK84_000569"
     CDS             complement(20522..21322)
                     /gene="btuF"
                     /locus_tag="KLK84_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HBE2008029.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21315..22013)
                     /gene="mtnN"
                     /locus_tag="KLK84_000570"
     CDS             complement(21315..22013)
                     /gene="mtnN"
                     /locus_tag="KLK84_000570"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBE2008030.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            22097..23614
                     /gene="dgt"
                     /locus_tag="KLK84_000571"
     CDS             22097..23614
                     /gene="dgt"
                     /locus_tag="KLK84_000571"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HBE2008031.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23744..25168
                     /gene="degP"
                     /locus_tag="KLK84_000572"
     CDS             23744..25168
                     /gene="degP"
                     /locus_tag="KLK84_000572"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HBE2008032.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25323..26479
                     /gene="cdaR"
                     /locus_tag="KLK84_000573"
                     /pseudo
     CDS             25323..26479
                     /gene="cdaR"
                     /locus_tag="KLK84_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26568..26954)
                     /locus_tag="KLK84_000574"
     CDS             complement(26568..26954)
                     /locus_tag="KLK84_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HBE2008033.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27268..28092)
                     /gene="dapD"
                     /locus_tag="KLK84_000575"
     CDS             complement(27268..28092)
                     /gene="dapD"
                     /locus_tag="KLK84_000575"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HBE2008034.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28123..30795)
                     /gene="glnD"
                     /locus_tag="KLK84_000576"
     CDS             complement(28123..30795)
                     /gene="glnD"
                     /locus_tag="KLK84_000576"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HBE2008035.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30857..31651)
                     /gene="map"
                     /locus_tag="KLK84_000577"
     CDS             complement(30857..31651)
                     /gene="map"
                     /locus_tag="KLK84_000577"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBE2008036.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32019..32744
                     /gene="rpsB"
                     /locus_tag="KLK84_000578"
     CDS             32019..32744
                     /gene="rpsB"
                     /locus_tag="KLK84_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBE2008037.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33002..33853
                     /gene="tsf"
                     /locus_tag="KLK84_000579"
     CDS             33002..33853
                     /gene="tsf"
                     /locus_tag="KLK84_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBE2008038.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34000..34725
                     /gene="pyrH"
                     /locus_tag="KLK84_000580"
     CDS             34000..34725
                     /gene="pyrH"
                     /locus_tag="KLK84_000580"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBE2008039.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDSTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35017..35574
                     /gene="frr"
                     /locus_tag="KLK84_000581"
     CDS             35017..35574
                     /gene="frr"
                     /locus_tag="KLK84_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBE2008040.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35666..36862
                     /gene="ispC"
                     /locus_tag="KLK84_000582"
     CDS             35666..36862
                     /gene="ispC"
                     /locus_tag="KLK84_000582"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBE2008041.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            37051..37809
                     /gene="ispU"
                     /locus_tag="KLK84_000583"
                     /gene_synonym="uppS"
     CDS             37051..37809
                     /gene="ispU"
                     /locus_tag="KLK84_000583"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HBE2008042.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37822..38679
                     /gene="cdsA"
                     /locus_tag="KLK84_000584"
     CDS             37822..38679
                     /gene="cdsA"
                     /locus_tag="KLK84_000584"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBE2008043.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38691..40043
                     /gene="rseP"
                     /locus_tag="KLK84_000585"
     CDS             38691..40043
                     /gene="rseP"
                     /locus_tag="KLK84_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HBE2008044.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPGSKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40073..42505
                     /gene="bamA"
                     /locus_tag="KLK84_000586"
     CDS             40073..42505
                     /gene="bamA"
                     /locus_tag="KLK84_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HBE2008045.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDVGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42627..43112
                     /gene="skp"
                     /locus_tag="KLK84_000587"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42627..43112
                     /gene="skp"
                     /locus_tag="KLK84_000587"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HBE2008046.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43116..44141
                     /gene="lpxD"
                     /locus_tag="KLK84_000588"
     CDS             43116..44141
                     /gene="lpxD"
                     /locus_tag="KLK84_000588"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HBE2008047.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44246..44701
                     /gene="fabZ"
                     /locus_tag="KLK84_000589"
     CDS             44246..44701
                     /gene="fabZ"
                     /locus_tag="KLK84_000589"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBE2008048.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44705..45493
                     /gene="lpxA"
                     /locus_tag="KLK84_000590"
     CDS             44705..45493
                     /gene="lpxA"
                     /locus_tag="KLK84_000590"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HBE2008049.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45493..46641
                     /gene="lpxB"
                     /locus_tag="KLK84_000591"
     CDS             45493..46641
                     /gene="lpxB"
                     /locus_tag="KLK84_000591"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HBE2008050.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46638..47234
                     /gene="rnhB"
                     /locus_tag="KLK84_000592"
     CDS             46638..47234
                     /gene="rnhB"
                     /locus_tag="KLK84_000592"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBE2008051.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47271..50753
                     /gene="dnaE"
                     /locus_tag="KLK84_000593"
     CDS             47271..50753
                     /gene="dnaE"
                     /locus_tag="KLK84_000593"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBE2008052.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTMRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50766..51725
                     /gene="accA"
                     /locus_tag="KLK84_000594"
     CDS             50766..51725
                     /gene="accA"
                     /locus_tag="KLK84_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBE2008053.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51824..53965
                     /gene="ldcC"
                     /locus_tag="KLK84_000595"
     CDS             51824..53965
                     /gene="ldcC"
                     /locus_tag="KLK84_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HBE2008054.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYHNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            54022..54411
                     /locus_tag="KLK84_000596"
     CDS             54022..54411
                     /locus_tag="KLK84_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBE2008055.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54476..55774
                     /gene="tilS"
                     /locus_tag="KLK84_000597"
     CDS             54476..55774
                     /gene="tilS"
                     /locus_tag="KLK84_000597"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBE2008056.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55823..56083)
                     /gene="rof"
                     /locus_tag="KLK84_000598"
     CDS             complement(55823..56083)
                     /gene="rof"
                     /locus_tag="KLK84_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HBE2008057.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56070..56270)
                     /locus_tag="KLK84_000599"
     CDS             complement(56070..56270)
                     /locus_tag="KLK84_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HBE2008058.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56436..56981
                     /locus_tag="KLK84_000600"
     CDS             56436..56981
                     /locus_tag="KLK84_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HBE2008059.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56978..57400
                     /gene="arfB"
                     /locus_tag="KLK84_000601"
     CDS             56978..57400
                     /gene="arfB"
                     /locus_tag="KLK84_000601"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBE2008060.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57414..58124
                     /gene="nlpE"
                     /locus_tag="KLK84_000602"
                     /gene_synonym="cutF"
     CDS             57414..58124
                     /gene="nlpE"
                     /locus_tag="KLK84_000602"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HBE2008061.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKTVLLSVEGHFTLEANPDTGVPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58279..59103)
                     /locus_tag="KLK84_000603"
     CDS             complement(58279..59103)
                     /locus_tag="KLK84_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HBE2008062.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59156..60874)
                     /gene="proS"
                     /locus_tag="KLK84_000604"
     CDS             complement(59156..60874)
                     /gene="proS"
                     /locus_tag="KLK84_000604"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBE2008063.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60985..61692)
                     /gene="tsaA"
                     /locus_tag="KLK84_000605"
     CDS             complement(60985..61692)
                     /gene="tsaA"
                     /locus_tag="KLK84_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HBE2008064.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61689..62093)
                     /gene="rcsF"
                     /locus_tag="KLK84_000606"
     CDS             complement(61689..62093)
                     /gene="rcsF"
                     /locus_tag="KLK84_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HBE2008065.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62211..63026)
                     /gene="metQ"
                     /locus_tag="KLK84_000607"
     CDS             complement(62211..63026)
                     /gene="metQ"
                     /locus_tag="KLK84_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HBE2008066.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(63066..63719)
                     /gene="metI"
                     /locus_tag="KLK84_000608"
     CDS             complement(63066..63719)
                     /gene="metI"
                     /locus_tag="KLK84_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HBE2008067.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63712..64743)
                     /gene="metN"
                     /locus_tag="KLK84_000609"
     CDS             complement(63712..64743)
                     /gene="metN"
                     /locus_tag="KLK84_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HBE2008068.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64931..65506
                     /gene="gmhB"
                     /locus_tag="KLK84_000610"
     CDS             64931..65506
                     /gene="gmhB"
                     /locus_tag="KLK84_000610"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HBE2008069.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            65870..>66140
                     /locus_tag="KLK84_000611"
     rRNA            65870..>66140
                     /locus_tag="KLK84_000611"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        15658 a        16369 c        18228 g        15885 t
ORIGIN      
        1 ggtacggaag tcactgtcgt tctcaaaatc ggtggagctg catgacaagg tcatcgggca
       61 ttatctgaac ataaaacact atcagtaagt tggagtcatt accggcgatg ggattgatat
      121 ttatctgcgg gaatttatat tatgtttctg caaaaatgtt taaactaaaa tttttattaa
      181 ggaaagagag gatgtatgat tttcagaaaa tatttatatt cgtaatataa ataaattacc
      241 atttgcttaa tttgtcaaat gctcttgtat gtaagacttc acgcaacgtc tccccatcag
      301 gaaacgccag ctccggtgca atctcaaaca gcggccacag cataaatcca cgattcttca
      361 tatcgtagtg cggaacggtc aggcgttcgg tatttatcac ttcattacca aacagcatga
      421 tatcgagatc cagcgtgcgt ggtccccagc gttcagcttt acgaacacga ccctgttgca
      481 gttctatgcg ctgtgtatgg ttgagtaact cttccggggc gggcgaagtt tcaagcgcca
      541 cggcggcgtt gaggtaatcg ggttgatctt gcggccccag cggtggggtg cggtaaaacg
      601 aagaaacggc aagaatgcgg ctttcaggga tatcgcctaa tgctttcagg gcagcattga
      661 cctgctccag cggagaggcc agattgctgc ctatggcaat atacgccact gtcatgtggt
      721 accctcacga cgcggtgcgc gtttgcgtgg acgacgagta cgacgacgcg gtgacggttc
      781 ttcatccagc tcgttaagca tccctttttg atctggtggt gcggaaacct ggaactcacc
      841 ccaccatttc accagacgct gcagttcagc attacgttca acttcagctc gcaaggccaa
      901 caggtcataa gccgcacgga acttaggatg ctccagcagt ttccatgcgc gtttaccctg
      961 acgacgggac atacgcaact gcaactgcca gatatcgcgg gttaacgtcg tcagacgttt
     1021 cgggattgcc agtgaacggc aggcttcgtc cagcacgtcg ttcatcgcca gcgcgaaagc
     1081 gtcgtgatag gtcaggccgc tttcctgggc gatcttctgt gccgtctcca gcagtgggta
     1141 ccagaacatg gcggcaaaca agaacgccgg gttcacgcgc atatcgttat ggatacgcgt
     1201 atcggtattc ttcagcacct gttcaatgat ccgctccatc gggctgtcgc cattttccgt
     1261 gaagtagcgg gtaatggtcg ggaacagcgg ctggaacaga tgatattcac acaacagctt
     1321 ataggtttcg taaccgtagc ccgcttgtag cagtttaagc gattcttcaa acaggcgtgc
     1381 cggtgggata tcgttcagca gggtagcgag gcgagggatc ggttctgcgg tttccgggct
     1441 gatgcgcata cccaatttgg cggcaaaacg taccgcgcgc agcatacgta ccggatcttc
     1501 acggtagcgc gtttccgggt taccaatcag acggataacg ccgtccttca gatccttcat
     1561 accaccaacg taatcacgga cggtaaaatc cgctacgctg taatacaggc tgttgatagt
     1621 gaaatcgcgg cgctgggcgt cttcttcgat ggagccgaaa atgttgtcgc gcagcaacat
     1681 gccgttttgc ccgcgttggg aggtcgtgcg gtcgctgacg ttaccttcgt ggtgtccacg
     1741 gaaggtcgca acttcgataa tctccgggcc aaacattaca tgagccagac ggaaacggcg
     1801 acccaccagg cggcagttac ggaacagttt gcgcacctgc tcaggcgtgg cgttagtggt
     1861 tacgtcaaaa tctttcggct ttttgccaag taacaggtcg cgcacgccgc cgccaaccag
     1921 ccaggcttcg tatcccgctt tgttgagcct gtacattacc ttcagggcat tttcactgat
     1981 atctttgcgg gaaatagcat gctgctcacg cgggatcacc gtcacctgtg gacgggcgac
     2041 tgcctgttca gcctcgcttt cctcgcggct tagcaccttg cggcaaaaat tagcgactcg
     2101 ggtaaaaata gtacacctcg gtagtgtcaa acatcattca ggacaaaaaa atagcggcta
     2161 atcatagctc agcatgacgc atttgagaat gttgaattta caattgccga ctcgggcacg
     2221 gcggtcagca tccagttttt gacggctgac tgaaggattt gctcgacgct gaaatcctgc
     2281 cagtgtgctt ctgcctgctg ccccagaaat tgaagtgccg cgattagtac cgggcgtgga
     2341 tcgcctttcg gcaacgcagg cgcatgattc tgcttggaaa gtttagcgcc ttgtggatta
     2401 agcgccagcg gcagatgaat gtaatcaggc actttccagc caaaaagctg gtacagcggg
     2461 atttgcctta ctgttggttc aatcagatca gccccacgca ctatttctgt aacgccctgg
     2521 aaatgatcat caaccacaac agccaggttg taggcgaaca acccatcacg gcgatgaatg
     2581 ataaaatctt cccgtgccag tttttcgtcg gcgtgaataa tgcctcgcag caggtcagta
     2641 aattgcgtga ccggatgctg ctggcggata cgcactgcgg cgttgtctgg tccatgatgc
     2701 aacacccggc aatgaccgtc gtaaataccg ccaatgcttt gaatacgcgc acgcgtacag
     2761 gtgcagtaat aacttagtcc ttgttcatgt aaccaggcga gtgcttcacg ataggcgtcg
     2821 tgacgttgcg attgccagag aacatcgccg tcccagtgca gaccgtaatg ttccagctgg
     2881 cgcaggatag tttctgcggc accgggaact tcacgaggcg ggtcgatatc ttctatgcgt
     2941 accagccagc gaccttgccg ggcgcgagcc tgcaaatagc tgccgagcgc ggcgatcaga
     3001 gagccaaaat gaagctcgcc ggaaggagag ggggcgaagc ggccaatata ctgtgtgtct
     3061 gtcatctctt tgaacaaaaa ataaggcggg agcatttccc gcctgtggta aacgtgatgg
     3121 aacggctgta attagccagc catctgtttt tcgcgaattt cagccagcgt tttgcagtcg
     3181 atgcacagat cggctgtcgg gcgcgcttcc agacggcgaa taccaatttc aacaccgcag
     3241 gattcgcagt agccgaaatc ttcgtcttcc acttttttca gcgtcttctc gatctttttg
     3301 atcagcttac gctcgcgatc gcggttacgc agttcgaggc tgaactcttc ttcctgggct
     3361 gcacggtcta ccgggtccgg gaagttggct gcttcatcct gcatatgtgt aacggtgcga
     3421 tcgacttcat ccctgagttg attacgccat gcttccagaa tacgacggaa gtgcgccagc
     3481 tgggcttcat tcatatactc ttcgcccggc ttctcctgat atggttccac cccagcgatg
     3541 gcgagaatac tcagggacga tgttttacgg ttttgccctt cttgcatgtt gcttctcctt
     3601 aacacgcact atcgatcccc atgttcgggg gaaaaatgag gccgctataa atagcagatg
     3661 cttttccgga tagcaattat ctaaacgtaa cacttgacaa ctgtgtgagg aaaagcgtat
     3721 ttgcgcacgc gaccagaatg taaattaacc agttacttac tttactacaa tgtaaccggc
     3781 agtgattttt taagagccat gccttcagca gaaatttccg ctttgtaagc cagaatttct
     3841 acccccctct gttgagcttc tgacaatagt tgcgcgtatt tctcatcgat gtggcgcgcg
     3901 ggtgaaaacc gtgtaatggc tgaatgcagc acggcgaaaa aaataaccgc acgctggcct
     3961 tcagccgcta cgctcatcaa ctcccgaagg tgtttctgac ctcgttcagt gaccgcatcg
     4021 ggaaaatatc cctgttcgtt ctccgctaac gtaaccgatt tcacttcaat atagcagtct
     4081 ggacgcgaat ccgcctgcaa cataaagtca atacggctgc gttcggagcc gtattttact
     4141 tcgcttttca gcgagctata gcctgacagt tctgaaattg attcattaag gatagcctct
     4201 ttcgtcaacc tgttagccca aagcgtattg acgcaaataa atgcgccgct ctggctttga
     4261 gttaattccc aggtgtgtgg gtatttccgt ttggtgttgt ctgaagtcga ataccagacg
     4321 gtatcgccag gcgttgcaca accggtcatc gcacccgtat tcgggcagtg tagcgttaat
     4381 tcgcgaccat cgggtgtgat cacatcggct aaaaaacgtt tgtaacgctg aattagcgtc
     4441 gcgcgctgta gagggggaga aaattccatt gcgacaatcc ttgttattgc gttagcgccc
     4501 agcgttttag cggcgtgtag cgtgtgcgtc cacgggcaaa cgaggaggcg taaagggtga
     4561 actccgtcac cgcataagac cagttaaaac ccggcggcgg gaatgtcacc gcctcgctgg
     4621 cgtcgcgcaa taaggtaata tgtggatgaa acggacgatt gctttgaaaa caaccgctgc
     4681 gggcagcctg tgaacggagc atattcgcca gctggattaa gccgcgtgga ggctgacgca
     4741 tccctaacca caccacacgc gaacgcagcc attgtccggc gtcatcaagc gtgagtgtga
     4801 aaccaggttg acgaatccgt ccggctaaaa gagaaagcgc cttctctttc tctgcgctca
     4861 cttcgcctaa aaatgccaga gtcaggtgca aattatcggc ggcgaccgga cgtcccgcct
     4921 caggtgggaa gtgtgtggcg cgccaatgga taatctgttc gcggatttct gcaggtaagt
     4981 cgatagcaaa gaacagacgt tgcggttcag acatgtgagg cactcggtta tgaattaccg
     5041 cgatgctaca atgtggcgcg aagaatgtta accctctgga gcgttttgtg tcgtcgttgc
     5101 ccgttgctgc cgtcttacct gaattactta ccgctctcga ttgtgcgccg caggtattat
     5161 taagtgcgcc gaccggggcc gggaaatcaa cctggctgcc gctgcaactg ctggcgcatc
     5221 ccggcattaa cgggaaaatt atcctgctgg agccgcgtcg tctggcggcg cgtaacgtcg
     5281 cgcaacggct ggcggagctg cttaacgaaa agccaggcga taccgttggc taccggatgc
     5341 gtgcgcaaaa ctgcgtcggg ctgaataccc gcctggaagt ggttaccgaa ggcgtgctga
     5401 cgcggatgat ccagcgtgac ccggaactga gcggtgttgg attggtgatc cttgatgagt
     5461 ttcatgagcg cagtttgcag gcggatttgg cgttggcgct gttactcgat gtgcaacaag
     5521 gtctgcgtga tgaccttaaa ctgctgatta tgtcggctac gctggacaat gaccgcttgc
     5581 agcaaatgct gccggaagcg cccgtcgtca tctcagaagg gcgctcgttt ccggttgaac
     5641 gccgttattt accgctgccc gcgcatcagc gttttgacga agccgttgcg gtagccaccg
     5701 ccgaaatggt gcgtcaggaa agtggatcat tactgttatt tttacctggc gtcggagaaa
     5761 ttcagcgcgt gcaggaacaa ctggcttcgc gcatcggcag tgatgtggtg ctctgcccgc
     5821 tgtatggcgt gttgtcactg aacgatcagc gaaaagcgat cctcccggca ccgcaaggga
     5881 tgcgcaaagt ggtgctggcg accaatattg ctgaaaccag tttaaccatc gaaggtattc
     5941 gtctggtggt ggattgtgcc caggagcgtg tggcgcgctt tgatccgcgc acggggctta
     6001 cgcggttgat tactcaacgc gttagtcagg catccatgac gcagcgtgcc gggcgcgccg
     6061 ggcgtctgga gccgggtatc agcctgcatt taatcgccaa agaacaagca gaacgcgccg
     6121 cggcgcaaag tgagccggag atcttacaaa gcgatctttc cggtttgctg atggaattac
     6181 tgcaatgggg atgcagcgat ccggcgcaga tgagctggct ggatcaaccg ccaacggtga
     6241 atctactggc cgcgaaacgc ctgttacaaa tgctgggggc gctggagggt gaacggctta
     6301 gtgcgcaagg gcaaaaaatg gcagcgctgg gtaacgatcc gcgtttagcg gcaatgctgg
     6361 ttaacgcaaa gagcgatgac gaagctgcta ccgcggcaaa aattgccgcc attctcgaag
     6421 agccgccacg gatgggcaat agtgacctgg gcgtggcgtt ttcgcgcaat caaccagcct
     6481 ggcagcaacg tagtcagcaa ctgttaaaac gcttaaacgt acgcggcggt gaggcagaca
     6541 gttcgcttat cgcgccgcta cttgccgggg cgtttgccga tcgcattgct catcgccgtg
     6601 ggcaagatgg acgctatcaa ctggcaaaca gcatgggagc gatgctcgat gccgacgacg
     6661 cgctaagccg ccacgaatgg ttgatcgcac cgttattatt gcagggcagc gcctcgccgg
     6721 atgcgcggat tttactggcg ctaccggtcg atattgatga attagtacaa cgctgtccgc
     6781 agctggtgca gcaatctgac accgtggaat gggatgacgc gcaaggtacg ctgaaagcct
     6841 ggcgtcggct acaaatcggt cagttgacgg tgaaagtgca gccgctggcg aaaccgtcag
     6901 aagacgagtt gcatcaggcg atgcttaatg gcatccgtga taaaggttta agcgtgctca
     6961 actggacggc ggaagcggaa cagctacgct tgcgtttgtt atgcgccgca aagtggttgc
     7021 cggaatatga ctggccagcg gttgatgatg aaagtttgtt ggcgacgctg gaaacgtggc
     7081 tgctgccaca tatgaccggc gtacattcac tacgcggcct gaaatcactc gacatttatc
     7141 aggcactacg cggattactt gattggggaa tgcagcaacg tctggatagt gaattgcctg
     7201 cgcattacac tgtgccgacg ggaagccgga tcgccattcg ttatcatgaa gataacccgc
     7261 ccgcgctggc ggtgagaatg caggagatgt ttggtgaggc cactaatccg acgatcgccc
     7321 aggggcgcgt gccgctggtg ctgaagttgc tttcccctgc ccaaaggccg ctgcaaatca
     7381 cgcgtgattt gggggccttc tggaaagggg cgtatcgtga ggtgcaaaag gagatgaaag
     7441 ggcgttatcc caaacacgtc tggccggacg acccggcaaa cacggcaccg acgcgacgga
     7501 cgaaaaagta ttcgtaaggt tggttttctc cctctccctg tgggagaggg ccggggtgag
     7561 ggcatcagcg cgcacgttca ccctcaccct aaccctctcc ctcaagggag aggggaccga
     7621 tcgagcgcaa attttgagag atatcttctt ctgtcttgta acagaagaac agaaaatcgg
     7681 gcttttgcgc ctgaatattg cggagaaaaa gcatggccgg gaatgaccgc gagccaattg
     7741 gacgcaaagg gaaaccgacg cgtccggtca aacaaaaggt aagccgtcgt cgttacgaag
     7801 atgacgatga ttacgacgat tatgatgact atgaggatga agaaccgatg ccgcgcaaag
     7861 gtaagggcaa aggcaaaggg cgtaagcctc gtggcaaacg cggctggcta tggctactgc
     7921 taaaactggc tatcgttttt gccgtgctga tcgccattta cggcgtttat ctcgatcaaa
     7981 aaattcgtag ccgtattgat ggcaaggtct ggcaactgcc cgcggcagtt tatggccgaa
     8041 tggtcaacct tgaaccagat atgaccatca gcaagaacga aatggtgaag ctgctggagg
     8101 caacacagta tcgtcaggtg tcgaaaatga cccgtcctgg cgaatttacc gtgcaggcca
     8161 acagcattga gatgatccgc cgtccgtttg atttcccgga cagtaaagaa gggcaggtgc
     8221 gtgcgcgtct gacctttgat ggtgatcatc tggcgaccat cgtcaatatg gagaacaacc
     8281 gtcagttcgg tttcttccgt cttgatccgc gtctgatcac catgatctcc tcgccaaacg
     8341 gtgagcagcg tttgtttgtg ccgcgcagtg gtttccctga tttgctggtg gatactttgc
     8401 tggcgacaga agaccgtcat ttttacgagc atgatggaat cagtctctac tccattgggc
     8461 gtgcggtgct ggcaaacctg accgcaggtc gcacggtgca gggtgcgagt acgctgacgc
     8521 aacagctggt gaaaaacctg ttcctctcca gcgagcgttc ttactggcgt aaagcgaacg
     8581 aagcttacat ggcgctgatc atggacgcgc gttacagcaa agaccgtatt cttgagctgt
     8641 atatgaacga ggtgtatctc ggtcagagcg gcgacaacga aatccgcggc ttcccgctgg
     8701 caagcttgta ttactttggt cgcccggtag aagagctaag cctcgaccag caggcgctgt
     8761 tagtcggtat ggtgaaaggg gcgtccatct acaacccgtg gcgtaacccg aaactggcgc
     8821 tggagcgacg taatctggtg ctgcgtctgc tgcaacagca acagattatt gatcaagaac
     8881 tctatgacat gttgagtgcc cgtccgctgg gggttcagcc gcgtggtggg gtgatctctc
     8941 ctcagccagc ctttatgcag ctggtgcgtc aggagttgca ggcaaaactg ggcgataagg
     9001 taaaagatct ctccggcgtg aagatcttca ctacctttga ctcggtggcc caggacgcgg
     9061 cagaaaaagc cgccgtggaa ggcattccgg cactgaagaa acagcgtaag ttgagcgatc
     9121 ttgaaactgc gattgtggtc gtcgaccgct ttagtggtga agttcgtgcg atggtcggag
     9181 gttctgagcc gcagtttgcg ggctacaacc gtgcgatgca ggcgcgtcgt tcgattggtt
     9241 cccttgcaaa accagcgact tatctgacgg ctttaagcca gccgaaaatc tatcgcctga
     9301 acacgtggat tgccgatgcg ccaatcgcgc tgcgtcagcc gaatggccag gtctggtcac
     9361 cgcagaacga tgaccgtcgt tatagcgaaa gcggcagagt gatgctggtg gatgcgttga
     9421 cacgttcgat gaacgtgccg acggtaaatc tggggatggc gctggggctg cctgcggtta
     9481 cggagacctg gatcaaactg ggcgtaccga aagatcagtt gcatccggtt ccggcaatgc
     9541 tgctgggggc gttgaactta acgccaatcg aagtggcgca ggcattccag accatcgcca
     9601 gcggtggtaa ccgtgcaccg ctttctgcac tgcgttcggt aatcgcggaa gatggtaagg
     9661 tgctgtatca gagcttcccg caggcagaac gcgctgttcc ggcgcaggcg gcgtatctga
     9721 cactatggac catgcagcag gtggtacaac gtggtacggg tcgtcagctt ggggcgaaat
     9781 acccgaacct gcatctggca gggaaaacag ggactaccaa caataacgta gatacctggt
     9841 ttgcaggcat tgacggcagc acggtgacca tcacctgggt aggccgtgat aacaaccagc
     9901 cgaccaaact gtatggagcc agcggggcaa tgtcgattta tcagcgttat ctggctaacc
     9961 agacgccaac gccgctgaat cttgttccgc cagaagatat tgcagatatg ggcgtggact
    10021 acgacggcaa ctttgtttgc agcggtggca tgcgtgtctt gccggtctgg accagcgatc
    10081 cgcaatcgct gtgccagcag agcgagatgc agcagcagcc gtcaggcaat ccgtttgatc
    10141 agtcttctca gccgcagcaa cagccgcaac agcaacctgc tcagcaagag cagaaagaca
    10201 gcgacggtgt agccggttgg atcaaggata tgtttggtag taattaacat ctaagcgtga
    10261 aataccggat ggcgagttgc catccggtaa aataacatcc catctaagat attaaccctt
    10321 tcttttcatc tggttgttta ttaacccttc aggaacgctc agattgcgta ccgcttgcga
    10381 acccgccagc gtttcgaata ttatcttatc tttataataa tcattctcgt ttacgttatt
    10441 attcacttta catcagagat ataccaatgg cgcgttccaa aactgctcag ccaaaacact
    10501 cactgcgtaa aatcgcagtt gtagtagcca cagcggttag cggcatgtct gtttatgcac
    10561 aggcagcggt tgaaccgaaa gaagacacta tcaccgttac cgctgcacct gcgccgcaag
    10621 aaagcgcatg ggggcctgct gcaactattg cggcgcggca gtcagctacc ggcactaaaa
    10681 ccgatacgcc gattcaaaaa gtgccacagt ctatttctgt tgtgaccgcc gaagagatgg
    10741 cgctgcatca gccgaagtcg gtaaaagaag cgcttagcta cacgccgggt gtctctgttg
    10801 gtacgcgtgg cgcatccaac acctatgacc acctgatcat tcgcggtttt gcggcagaag
    10861 gccaaagcca aaataactat ctgaatggcc tgaagttgca gggcaacttc tataacgatg
    10921 cggtcattga cccgtatatg ctggaacgcg ctgaaattat gcgtggcccg gtttccgtgc
    10981 tttacggtaa aagcagtcct ggcggtctgt tgaatatggt cagcaagcgt ccgaccaccg
    11041 aaccgctgaa agaagttcag tttaaagccg gtactgacag cctgttccag actggttttg
    11101 actttagcga tgcgttggat gatgacggtg tttactctta tcgcctgacc ggtcttgcgc
    11161 gttctgccaa tgcccagcag aaagggtcag aagagcagcg ttatgctatt gcaccggcgt
    11221 tcacctggcg tccggatgat aaaaccaatt tcaccttcct ttcttacttc cagaacgagc
    11281 cggaaaccgg ttattacggc tggttgccga aagagggaac cgttgagccg ctgccgaacg
    11341 gtaagcgtct gccgacagac tttaatgaag gggcgaagaa caacacctat tctcgtaatg
    11401 agaagatggt cggctacagc ttcgatcacg aatttaacga cacctttact gtgcgtcaga
    11461 acctgcgctt tgctgaaaac aaaacctcgc aaaacagcgt ttatggttac ggcgtctgct
    11521 ccgatccggc gaatgcttac agcaaacagt gtgcggcatt agcgccagcg gataaaggcc
    11581 attatctggc acgtaaatac gtcgttgatg atgagaagct gcaaaacttc tccgttgata
    11641 cccagttgca gagcaagttt gccactggcg atatcgacca caccctgctg accggtgtcg
    11701 actttatgcg tatgcgtaat gacatcaacg cctggtttgg ttacgacgac tctgtgccac
    11761 tgctcaatct gtacaatccg gtgaatcccg atttcgactt caatgccaaa gatccggcaa
    11821 actccggccc ttaccgcatt ctgaataaac agaaacaaac gggcgtttat gttcaggatc
    11881 aggcgcagtg ggataaagtg ctggtcaccc taggcggtcg ttatgactgg gcagatcaag
    11941 aatctcttaa ccgcgttgct gggacgaccg ataaacgtga tgacaaacag tttacctggc
    12001 gtggtggtgt taactacctg tttgataatg gtgtaacacc ttacttcagc tatagcgaat
    12061 cgtttgaacc ttcttcgcaa gttgggaagg atggtaatat tttcgcaccg tctaaaggta
    12121 agcagtatga agtcggcgtg aaatatgtac cggaagatcg tccgattgta gttactggtg
    12181 ccgtgtataa tctcactaaa accaacaacc tgatggcgga ccctgagggt tccttcttct
    12241 cggttgaagg tggcgagatc cgcgcacgtg gcgtagaaat cgaagcgaaa gcggcgctgt
    12301 cggcgagtgt taacgtagtc ggttcttata cttacaccga tgcggaatac accaccgata
    12361 ctacctataa aggcaatacg cctgcacagg tgccaaaaca catggcttcg ctgtgggctg
    12421 actatacctt ctttgacggt ccgctttcag gtctgacgct gggcaccggt ggtcgttata
    12481 ctggctccag ctatggtgat ccggctaact cctttaaagt gggaagttat acggtcgtgg
    12541 atgcgttagt gcgttatgat ctggcgcgag tcggcatggc gggctccaac gtggcgctgc
    12601 atgttaacaa cctgttcgat cgtgaatacg tcgccagctg ctttaacact tatggctgct
    12661 tctggggcgc agaacgtcag gtcgttgcaa ccgcaacctt tcgtttctaa tttctctttt
    12721 ggggcacgga tttccgtgcc catttcacaa gttggctgtt atgcaggaat acacgaatca
    12781 ttccgatacc acttttgcac tgcgtaatat ctcctttcgt gtgcccgggc gcacgctttt
    12841 gcatccgctg tcgttaacct ttcctgccgg gaaagtgacc ggtctgattg gtcacaacgg
    12901 ttctggtaaa tccactctgc tcaaaatgct tggtcgtcat cagcctccgt cagaagggga
    12961 gattcttctt gatgcccagc cgctggaaag ctggagcagc aaagcgtttg cccgcaaagt
    13021 ggcttatttg ccgcagcagc ttcctccggc agaagggatg accgtgcgtg aactggtggc
    13081 gattggtcgt tacccgtggc atggcgcgct ggggcgcttt ggtgcggcag atcgcgaaaa
    13141 ggtcgaggaa gctatctcgc tggttggctt aaaaccgctg gcgcatcggc tggtcgatag
    13201 tctctctggc ggcgaacgtc agcgggcgtg gatcgccatg ctggtggcgc aggatagccg
    13261 ttgtctgttg ctcgacgaac cgacttcagc gctggatatc gcccaccagg ttgatgtgct
    13321 ggcgctggtg caccgtttaa gtcaggaacg tggcctgacg gtcattgccg tgttgcacga
    13381 tatcaatatg gcggcacgct actgtgatta tctggtcgcc ctgcgcagtg gtgaaatgat
    13441 tgctcaggga acgcctgcgg aaattatgcg cggcgaaacc ctcgaaatga tttatggcat
    13501 cccgatgggt attttgccgc atccggcggg tgctgcacct gtgagttttg tttattgatg
    13561 agcggcttac ctcttatttc gcgccgtcga ctgttaacgg cgatggcgct ttctccgttg
    13621 ttatggcaga tgaataccgc ccacgcggcg gctattgacc ccaatcgtat tgtggcgctg
    13681 gagtggttgc cggtggaatt actgctggcg ctcggcatcg tgccttacgg cgtggcggat
    13741 accatcaact atcgcctgtg ggtcagcgaa ccaccattgc cggactcagt gattgacgtc
    13801 ggtttgcgta cagaacctaa ccttgaactg ctgaccgaaa tgaaaccgtc gtttatggtc
    13861 tggtcggcag gatatggccc ttcatcagaa atgctggcac gtattgcgcc gggtcgcgga
    13921 tttaacttca gtgacggcaa acatccgttg gcgatggcgc gtaaatcgct gacggaaatg
    13981 gcagatttac ttaacctgca aagcgcagcg gaaacgcatt tagcgcaata cgaagacttt
    14041 atccgcagca tgaaaccccg ctttgtgaag cgtggtgcac gcccgttact gctgacgacg
    14101 cttatcgatc cgcgccatat gctggtcttc ggtccaaaca gcttgttcca ggaaattctc
    14161 gatgagtacg gcatcccaaa tgcctggcaa ggggaaacca acttctgggg cagtaccgcc
    14221 gtcagtatcg atcgtctggc agcgtataaa gacgttgatg tgctctgttt tgatcacgac
    14281 aacagcaaag acatggatgc gctaatggca accccgctgt ggcaggccat gccgtttgtc
    14341 cgtacagggc gctttcagcg cgtacccgca gtctggtttt atggtgcaac gctttcggca
    14401 atgcactttg tgcgtgttct ggataacgcc atcggaggta aagcgtgagt aaacgaattg
    14461 cgcttttccc ggcgttattg ctggcgctgt tagtgattgt cgctacggcg ctcacctgga
    14521 tgaacttctc acaggcgctg ccgcgcagtc agtgggcgca ggccgcctgg tcgccggata
    14581 ttgacgtcat cgagcagatg atttttcact acagcttgtt gccgcgtctg gcgatttcgc
    14641 tgctggtggg cgcgggcctg gggctggtgg gcgtgctgtt tcagcaagta ctgcgtaacc
    14701 cgctggcgga accgacgacg ttgggcgttg caacaggcgc gcaactgggg attaccgtca
    14761 ccacgctctg ggcgatccct agcgcgatgg cgagccagtt tgctgcgctg gcaggggctt
    14821 gcgttgttgg cttaattgtc tttggcgtcg cgtgggggaa acggctgtcg ccggtaacgc
    14881 tgattttcgc ggggttggta gtgagccttt attgcggcgc aatcaatcag ttactggtta
    14941 tcttccatca tgaccagttg caaagcatgt ttctgtggag cactggaacg ctgacgcaaa
    15001 ccgactgggg cggtgttgag cgtttatggc cgcagttgct gggcggtgtg atgctgacgt
    15061 tgctgctact tcgtccgtta accctgatgg ggcttgatga tggcgtggcg cgcaatctcg
    15121 ggctggcctt gtcgcttgcg cgtctggcgg cgctgtcgct ggcgattgtc atcagtgcgc
    15181 tgctggtgaa cgctgtgggg attatcggct ttatcgggtt gttcgcgccg ctgctggcaa
    15241 aaatgctggg ggcgcggcgt ctgctgccac gactgatgct ggcgtcgttg attggtgcgc
    15301 tgatcctctg gctttccgat caaatcatcc tctggctgac tcgcgtgtgg atggaagtgt
    15361 ccaccggttc ggtcactgcg ttgatcggtg cgccgctgct actgtggctg ctgccgcgtt
    15421 tacgcagcat tagcgcgccg gatatgaagg ttaacgatcg tgtcgcgact gaacgccaac
    15481 atgtgctggc gtttgccctc gcgggcggcg tgctgttgtt gatggctgtg gtggtggcgc
    15541 tgtcgtttgg tcgtgatgcg cacggctgga cgtgggcgag cggggcgttg ctcgaggatt
    15601 taatgccctg gcgctggccg cgaattatgg cggcgctgtt tgcgggcgtc atgctggcgg
    15661 tagcgggctg tattattcag cgactgaccg gcaacccgat ggcaagcccg gaagtgctgg
    15721 ggattagctc cggcgcggcg tttggtgtgg tgttaatgct gtttctggta ccgggtaatg
    15781 cctttggctg gttgttaccc gcaggcagtc tcggggcggc ggtgacgctg ttgatcatta
    15841 tgatcgccgc cggacgcggt ggattttccc cacaccgcat gttactggcg gggatggcgt
    15901 taagtaccgc gttcaccatg cttttgatga tgttgcaggc aagtggtgac ccgcgaatgg
    15961 cgcaagtgct gacctggatt tccggttcga cctacaacgc gaccgatgct caggtctggc
    16021 gcaccggaat tgtgatggtg attttgctgg cgattactcc gctgtgccgc cgctggctga
    16081 ccattttacc gctgggtggt gataccgcgc gggcggtagg gatggcgctg acgccgacgc
    16141 gaattgcgct gctgctgtta gcggcttgcc tgacggcgac cgcgacgatg actattggac
    16201 cgttgagttt tgttggttta atggcaccgc atattgcgcg gatgatgggc tttcgacgga
    16261 cgatgccaca catcgtaatt tcggcgctgg tgggtggttt actgctggtg ttcgctgact
    16321 ggtgtgggcg gatggtgctg tttccattcc agatcccggc ggggctgctg tcgaccttta
    16381 tcggtgcgcc atattttatc tatttgctga gaaagcagag ccgttaatag aatgcctcgc
    16441 ccggtgggcg aggcaaaatt atcacaactt cgcgaacacc cgacgtgcag catcgatggt
    16501 gttattgata tcttccatgc tgtgcgccac agacataaag cccgcttcaa acgctgacgg
    16561 tgccaggtaa acaccttcat ccagcatcat atggaagaaa cgcttaaaac gctcgacgtc
    16621 acaggccatc acatcctgat agcacgtcac ggactcggcg tcggtaaaga agataccgaa
    16681 cataccaccg acgtggttaa cgaccagcgg aattcccgct tcttctgccg cttccagcag
    16741 accttctgcc agacgtgttg tcagctcatc cagcgtttcg tgaatgcccg gctgcgcgac
    16801 ttcattcaga caggcgaaac ccgctgccat cgcaatcggg ttaccggaaa gcgtacccgc
    16861 ctgatagacc ggacctgtcg gggccagcgc atccattaca tcacgacgac caccgaatgc
    16921 gcctaccggc attccaccgc cgatgatttt tccgaggcag gttaaatctg gcactacggc
    16981 gaaataatcc tgtgcgccag ctagcgctac gcggaaaccg gtcatcactt catcgatgat
    17041 cagcaacgcg ccaaattcgt cgcacagcgc acgcagacct ggcaggaact ctggcagcgg
    17101 cggaacacag ttcatattgc ctgccaccgg ctcgacgata atacaggcaa tctcttgcgg
    17161 gtattgctca aacgcggcgc gtacagaggc cagatcgtta taagtacagg ttaaggtatg
    17221 tttggcgaaa tctgccggaa cgcccggcga gtttggctgg cctaacgtga gtgcgccaga
    17281 accggctttc accagcaggc agtcagcgtg accgtggtaa caaccttcaa atttaatgat
    17341 tttgtcgcga ccggtaaaac cacgggccag gcggatggcg ctcatcgtcg cctcggtgcc
    17401 ggagttcacc atgcgcacca tatccatggt cgggaccagt tcggtcacca gttgcgccat
    17461 tttcacttcc atttcggtcg gtgcaccaaa gcttaaacca cgctcggcgg cttcaatcac
    17521 ggcattgcgg atcgccggat ggttatggcc cagcaccatc ggcccccagg aaccgacata
    17581 atcgatatag gctttgccat caacatcgta cagataagcg ccgtccgctt tttcgataaa
    17641 cagtggagtg ccgcccacgc cagtaaaggc gcgaacaggg gagttcacac cgccagggat
    17701 cagctcgcgc gctgcgctgt aaagattttc agacttactc atggagggtt cctgattcgt
    17761 agaaaaagtg aatggctgct attccatgtt attcataaca agttaaatac tcgtcaaaca
    17821 tcaggctgct tgtactggtg caatcctgat ttcttagagt ataaaagttt tgtgcatttg
    17881 aaacattacg ctttgcaaag gattttcatg gaacgtgcga gtaaaatgcc gtcatcttat
    17941 ttgtatgacc aataagtgat cattggatga aaactgatac tccctcttta gaaacaccgc
    18001 aggccgcgcg cctgcgacgc agacaactga ttcgccaact tcttgagcgc gataaaaccc
    18061 cgttagccat tttgtttatg gcggcagtcg tcggcacgct tgtcgggctg gctgcggttg
    18121 cttttgacaa aggtgtcgcc tggttgcaga accaacgtat gggggcgctg gtacatactg
    18181 ctgataatta tccgctactg ttaaccgtcg cttttctctg ttcggcggtg ctggcgatgt
    18241 tcggctactt tttggtgcgc aaatacgcgc ccgaagcagg gggatcgggg atcccggaaa
    18301 ttgaaggggc gctggaagat caacgtcctg ttcgctggtg gcgtgtattg ccggtgaaat
    18361 tctttggcgg gctggggaca ctcggcggag gcatggggct agggcgcgaa ggaccaaccg
    18421 tgcagatcgg cggcaatatc agtcgcatgg tgcttgatat tttccgcctg aaaggtgatg
    18481 aagctcgcca tacgctgctg gcaaccggtg ctgctgcggg gctggctgcg gcctttaacg
    18541 cgccgctggc gggtattttg tttattatcg aagagatgcg tccgcagttt cgctatacgt
    18601 taatttcgat taaagcggta tttattggcg tcattatgtc gaccattatg taccggattt
    18661 ttaatcatga agttgcgttg attgacgtcg gtaaactttc tgatgcgccg cttaatacgc
    18721 tgtggcttta tctgatcctc ggtattattt ttggcatttt cggccctatt tttaataaat
    18781 gggtgctggg gatgcaggat ttgctgcacc gtgtgcacgg cggcaatatt accaaatggg
    18841 tactgatggg gggtgcgatt ggcggtctgt gtggattgct ggggtttgtg gcaccagcaa
    18901 cgtcgggcgg cggttttaac ctgattccta tcgctaccgc ggggaatttc agcatgggaa
    18961 tgctggtgtt tatcttcgtc gctcgggtca ttaccacctt actctgcttc tcttccggcg
    19021 cgccgggcgg tatttttgcc ccgatgctgg cgctgggtac tgtgctggga accgctttcg
    19081 gaatggttgc cgttgagctg tttccgcaat atcacctgga ggcggggacg tttgctattg
    19141 ccggaatggg ggcattactg gcggcatcta ttcgcgcgcc gttaacgggg atcattctgg
    19201 ttctggagat gaccgataac taccagctca ttttgccaat gattattacc ggtcttggcg
    19261 caacactatt agcgcaattt accggcggga aacctctata ctcggcgatt cttgcgcgca
    19321 cgctggcaaa acaggaagct gagcaactgg cgcgaagcaa ggccgcatca gccagcgaga
    19381 atacttgaac gaaataccag ggtattagat aatggcgatt attattgggt tagaatttgc
    19441 ccgattgccg atgtcgtttg gagcaaaata tgagtgatga cgtagcactg ccgctggagt
    19501 ttaccgacgc agcagccaac aaagttaaaa gcctgatcgc tgacgaagat aacccgaatc
    19561 tgaaattacg cgtgtatatc accggtggcg gttgcagcgg cttccagtat ggtttcacct
    19621 ttgatgatca ggtgaacgaa ggcgatatga ccatcgaaaa acagggcgtt ggcctggtgg
    19681 ttgatccgat gagcctgcaa tatctggtcg gcggttccgt tgactacacc gaaggactgg
    19741 aaggttctcg tttcatcgtg accaacccga acgcgaaaag cacctgcggt tgcggttctt
    19801 cctttagcat ctaatctgtt gttcatgtgc cggataaact atccggcaca tcttctctct
    19861 tcaacgccca ttctcatcca gcgcaaacgt cggtagctta agatgccaac gaatagccgc
    19921 cagccgaatc aatagcgtca cgaccatgcc catcatactg gctgtttcca gtggtacgga
    19981 aaatgtgtaa taagccgtag cgtggacaat accgccgata atacaggcag ttgcgtagat
    20041 ttctgtacgt aaaatcatgg ggatttcgcg gaccagtaca tcacgaatga tcccgccgcc
    20101 aacgccagta atgacgccca tacaaaccgc gattaacgga ccggcttccg cattaaaggc
    20161 tttattcacg ccaatgccga caaacaccgc cagaccaacg gcgtccagca ccggcaacat
    20221 ccattttggt aagcgtcttg gctggcgcac cagcacgatg gtcagcatgc tggtgaccat
    20281 ggcaacgacc agatcggtgg gatctttcac ccaaaatacc gggccgtgat ccagcgccat
    20341 gtcgcgaatt gttccgtcgc ctactgcggt aaccacgccc agtaccagaa caccaaaagg
    20401 gtccatacgc aattttccgg ctaacaaaac gccgtagatg gcaaataccg ctgtgccgac
    20461 tatatccagc caatagacga gcattgttca atccccactg agcacctgaa aaggtcaggc
    20521 actaatctac ctgtgaaagc gcattacaga gctgttgtgc tgcgaggata atacgtgggc
    20581 ttgcacgttc aaaccagtca ctcgtgagag gaataacggg aattttgagc tgttcacccc
    20641 agtattgttt gattttagga atttggtccg gtccgcctgt aatgacaata gcctgtggcg
    20701 agcgtgctaa cacctgttcg cggctaactt gcggccaggg aacccggctg tctttaaaga
    20761 tgttttctcc gccacaaact tcgagtacct ggttctgaat cgactctttt ccactggtaa
    20821 atggcggatt aatgccgaat tgcagaaaaa cacgtttttt aggtttatca gcatattgcg
    20881 ctttcaattg cacgtactga tccagcaggg attgcgcggc ttgttcggcc ttgtctggtt
    20941 gcggactcca gggggccagt tgacgtaacg cattggcaat ttgttcaatg cttgtcgcat
    21001 cgacccacat cacttttatt cccagcgaag ccagctggtc aacctgccgc tcggcattac
    21061 ctccacgcca ggcaatcacc agatcgggtt tcagcgcgac aatgcgttcc agattcatcc
    21121 cctgccaggt ggaaacctgc tcaatctttt gcgcttgtgg aggatagtcg gaatagctgc
    21181 tgaccccaac cggcgtgatc ccggcggcaa aggcaagttc agtgttggcg ggagaaagcg
    21241 tgatgacgcg cggcgcggcg ttgagccaca gtggcgcgag aaaagacagg gcgaccagcg
    21301 ccctgaacag tgatttagcc atgtgccagt ttctgcacca gtgactcaac catcaggctg
    21361 gactgtttag cggcaacagc caggaactca tcgaagctaa gatgagactg ttgatcggcc
    21421 acgtcggaga tggcgcgtac gacaacaaac gggacgttga aattgtggca gacatgggcg
    21481 attgccgtcg cttccatctc tacagcaatg gcctgtggga agttgtggcg gattttcgcc
    21541 agaccaacag aaccgttgat gaaagcgtcg ccgctaacaa tcaggccacg tacagcgtta
    21601 agattcagtt cggcaatgca ggcctcagcg gcagcgatca gtttatcgtc agctttaaag
    21661 cctgccggac agcctggtaa ctgaccgtat tcataaccaa atgccgtgac atccgcgtcg
    21721 tgataacgcg cttcgtccga gacaacgata tcgcccactt tcagcgttgg tgccaggcca
    21781 ccggcagaac cggtgttaat aatcacatct ggcttgcagt gttccaacag cagagtggca
    21841 cccagcgccg cagcgacttt accgatgccc gatttcagaa gcgcaacctc ggttccattc
    21901 agttggccgg tatagatttc gcaaccgccg agactgatag tttgacggtt ttcgattttg
    21961 tcacgcagca gcgtaacttc ttcttccatt gcaccaatga tgccgatttt catagattta
    22021 ctcgcgataa gcccgatttg aaggcatagt ttaccatgcg cttacgggga agcgtatttc
    22081 tcacgcggga gaggacatgg cacagattga tttccgaaaa aaaataaact ggcatcgtcg
    22141 ttaccgttca ccgcagggcg ttaaaaccga acatgagatc ctgcggatct tcgagagcga
    22201 tcgcgggcgt atcatcaact ctccggcaat tcgtcgtctg caacaaaaga cccaggtttt
    22261 tccactggag cgcaatgccg ccgtgcgcac gcgtcttacc cactcgatgg aagtccagca
    22321 ggtgggacgc tacatcgcca aagaaatttt aagccgtctg aaagagctta aattgctgga
    22381 agcatacggc ctggatgaac tgaccggccc ctttgaaagc attgttgaga tgtcatgcct
    22441 gatgcacgat atcggcaatc cgccgtttgg tcattttggc gaagcagcga taaatgactg
    22501 gtttcgccaa cgtttgcacc cggaagatgc cgaaagccag cctctgactg acgatcgctg
    22561 cagcgtggcg gcactacgtt tacgggacgg ggaagaaccg cttaacgagc tgcggcgcaa
    22621 gattcgtcag gacttatgtc attttgaggg gaatgcacaa ggcattcgtc tggtgcatac
    22681 attgatgcgg atgaatctca cttgggcaca ggttggcggt attttaaaat atacccgtcc
    22741 ggcgtggtgg cgtggcgaaa cgcctgagac acatcactat ttaatgaaaa agccgggtta
    22801 ttatctttct gaagaagcct atattgcccg cttgcgtaaa gaacttaatt tggcgcttta
    22861 cagtcgtttt ccattaacgt ggattatgga agctgccgac gacatctcct attgtgtggc
    22921 agaccttgaa gatgcggtag agaaaagaat atttaccgtt gagcagcttt atcatcattt
    22981 gcacgaagcg tggggccagc atgagaaagg ttcgctcttt tcgctggtgg ttgaaaatgc
    23041 ctgggaaaaa tcacgctcaa atagtttaag ccgcagtacg gaagatcagt tttttatgta
    23101 tttacgggta aacaccctaa ataaactggt accctacgcg gcacaacgat ttattgataa
    23161 tctgcctgcg attttcgccg gaacgtttaa tcatgcatta ttggaagatg ccagcgaatg
    23221 cagcgatctt cttaagctat ataaaaatgt cgctgtaaaa catgtgttta gccatccgga
    23281 tgtcgagcag cttgaattgc agggctatcg ggtcattagc ggattattag agatttatcg
    23341 tcctttatta agcctgtcgt tatcagactt tactgaactg gtagaaaaag aacgggtgaa
    23401 acgtttccct attgagtcgc gcttattcca caaactctcg acgcgccatc ggctggccta
    23461 tgtcgaggct gtcagtaaat taccgtcaga ttctcctgag tttccgctat gggaatatta
    23521 ttaccgttgc cgcctgctgc aggattatat cagcggtatg accgacctct atgcgtggga
    23581 tgaataccga cgtctgatgg ccgtagaaca ataaccaggc ttttgtaaag acgaacaata
    23641 aatttttacc ttttgcagaa actttagttc ggaacttcgg gctataaaac gaatctgaag
    23701 aacacagcaa ttttgcgtta tctgttaatc gagactgaaa tacatgaaaa aaaccacatt
    23761 agcactgagt gcactggctc tgagtttagg tttggcgtta tctccgctct ctgcaacggc
    23821 ggctgagact tcttcagcaa cgacagccca gcagatgcca agccttgcac cgatgctcga
    23881 aaaggtgatg ccttcagtgg tcagcattaa cgtagaaggt agcacaaccg ttaatacgcc
    23941 gcgtatgccg cgtaatttcc agcagttctt cggtgatgat tctccgttct gccaggaagg
    24001 ttctccgttc cagagctctc cgttctgcca ggggggccag ggcggtaatg gcggcggcca
    24061 gcaacagaaa ttcatggcgc tgggttccgg cgtcatcatt gatgccgata aaggctatgt
    24121 cgtcaccaac aaccacgttg ttgataacgc gacggtgatt aaagtccagc tgagcgatgg
    24181 tcgtaagttc gacgcgaaga tggttggcaa agatccgcgc tctgatatcg cgctgatcca
    24241 aatccagaac ccgaaaaaac tgaccgcaat taagatggcg gattctgatg cactgcgcgt
    24301 gggtgattac accgtagcga ttggtaaccc gtttggtctg ggcgagacgg taacttccgg
    24361 gattgtctct gcgctggggc gtagcggcct gaatgctgaa aactacgaaa acttcatcca
    24421 gaccgatgca gcgatcaacc gtggtaactc cggtggtgcg ctggttaacc tgaacggcga
    24481 actgatcggt atcaacaccg cgatcctcgc accggacggc ggcaacatcg gtatcggttt
    24541 tgctatcccg agtaacatgg tgaaaaacct gacctcgcag atggtggaat acggccaggt
    24601 gaaacgcggt gagctgggta ttatggggac tgagctgaat tccgaactgg cgaaagcgat
    24661 gaaagttgac gcccagcgcg gtgctttcgt aagccaggtt ctgcctaatt cctccgctgc
    24721 aaaagcgggc attaaagcgg gtgatgtgat cacctcactg aacggtaagc cgatcagcag
    24781 ctttgccgca ctgcgtgctc aggtgggtac tatgccggtg ggcagcaaac tgaccctggg
    24841 cttactgcgc gacggtaagc aggttaacgt gaacctggaa ctgcagcaga gcagccagaa
    24901 tcaggttgat tccagctcca tcttcaacgg cattgaaggc gctgagatga gcaacaaagg
    24961 caaagatcag ggcgtggtag tgaacaacgt gaagacgggc actccggctg cgcagatcgg
    25021 cctgaagaaa ggtgatgtga ttattggtgc gaaccagcag gcagtgaaaa acatcgctga
    25081 actgcgtaaa gttctcgaca gcaaaccgtc tgtgctggca ctgaacattc agcgcggcga
    25141 cagcaccatc tacctgttaa tgcagtaatc tccctcaacc ccttcctgaa tacgggaagg
    25201 ggttctcctt acaatctgtg aacttcgcca caactccata catcttcatc atcctttggg
    25261 catttgcaca atgccgtacg ttacgtactt ccttatgcta agccgtgcat aacggaggac
    25321 ttatggctgg ctggcatctt gataccaaaa tggcgcagga tatcgtggca cgtaccatgc
    25381 gcatcatcga taccaatatc aacgtaatgg atgcccgcgg gcgaattatc ggcagcggcg
    25441 atcgtgagcg tattggtgaa ttgcacgaag gtgcattgct ggtactttca cagggacgag
    25501 tcgtcgatat cgatgacgcg gtagcacgtc atctgcacgg tgtgcggcag gggattaatc
    25561 taccgttacg gctggaaggt gaaattgtcg gcgtaattgg cctgacaggt gaaccagaga
    25621 atctgcgtaa atatggcgaa ctggtctgca tgacggctga aatgatgctg gaacagtcgc
    25681 ggttgatgca cttgttggcg caggatagcc gtttgcggga agaactggtg atgaacctga
    25741 ttcaggcaga ggagaatact cccgcacttc tgaatgggcg caacggctgg ggatcgatct
    25801 caatcaaccg cgagtggtgg ctattgttga ggtcgacagc ggtcagcttg gcgtggacag
    25861 cgcaatggcg gagttacaac aactgcaaaa cgcgctgact acgcccgagc gtaataatct
    25921 ggtggcgatt gtctcgctaa ccgaaatggt ggtgttgaaa ccggcgttga actcttttgg
    25981 gcgctgggat gcagaagatc atcgtaagcg agttgaacaa ctgattaccc gcatgaaaga
    26041 gtacggccag ctgcgttttc gcgtttcact gggcaactat tttaccggtc ctggcagtat
    26101 tgcccgatcc tatcgtacgg cgaaaacgac gatggtggtg ggtaaacagc ggatgccaga
    26161 aagtcgctgc tatttttatc aggatctgat gttacctgtg ttactcgaca gtttgcgtgg
    26221 cgactggcag gccaacgaac tggcgcgacc gctggcgcgg ctgaaagcga tggacaataa
    26281 cggcttgctg cgacgaacgc tggcggcgtg gtttcgccac aatgtgcaac cgctggcaac
    26341 gtcaaaggcg ttgtttattc atcgtaatac cctggagtat cggcttaatc gtatatcgga
    26401 actgaccggg cttgatttgg gcaattttga tgacaggttg ctgctgtatg tggcgttaca
    26461 actggatgaa gagcggtaga ttatgcgtta aggttgaggc agcgctggct cattcccctc
    26521 agcccgaagg ggagagggga atgcccgagt taaccgtcaa ttcgggatta tttacgcgtt
    26581 agtttttcca gatcggcttc aatctcgctg atcttattgg ttacgactga ctccaggtga
    26641 cgtaagtcgt cgaggatctt acgcttaaga tcaacttcgc tgcggtcacg ctgacagatt
    26701 tgatcaagct catcaatgat ataccgtaga ttcgggctga tttcctggac ttctttataa
    26761 ccctgaccca caccatcagc gacgaccgtt ttacgctgac gcggatattt aaacttaacg
    26821 ctcttggcga aaaactcgcc tttgtctttc tggaaataga ttttcagaat atcgttgttg
    26881 gcttcctgcc ggaggctgta acgatcaatt tcttcaggat tggtaatacc cagacttttc
    26941 agattgtcgt acatagcgtt acctcaaaat gagtcagtaa attgtgctta ttttagcatt
    27001 tggcttggcc cgcccggctc gactgtgatc gcagaaagcc tttttcttcc agacttctat
    27061 catggcgtaa aaatcaaaaa attacctgct ttattgtggc gatattccat gggatggatt
    27121 agcacattta tcttcgtttt gcgaattaca tgacagtccg aaacaagatg aaaaagtatt
    27181 taaaaaaggg ggaggaagaa tgagtaggac gaattattct gagccagaaa gcatacgggc
    27241 agcatcatgc tgcccgtata tgtactctta atcgatggta cgcagcagtt cgttaatgcc
    27301 gactttgccg cgagttttcg cgtcaacttt cttaacgata accgcacagt agaggctgta
    27361 tttgccatct tttgacggca gattacctga aacaaccaca gaccccgccg gaacgcgacc
    27421 gtagtggatt tcgccggttt cacggtcgta aatacgggtg ctctgaccaa tgtatacgcc
    27481 catagagatg acggaacctt cttcgacaat caccccttca accacttcag agcgtgcgcc
    27541 aatgaagcag ttatcttcaa taatggtcgg gttagcctgc agcggctcca gcacgccgcc
    27601 gatgcccacg ccaccggaaa ggtggacgtt tttaccaatc tgcgcacaag aaccgacggt
    27661 cgcccaggta tcaaccatgg tgccttcatc aacatatgcg ccgatgttaa cgtaagacgg
    27721 catcagcacg gtgttacggg caataaacgc accctgacgt accgccgctg gtggcacaac
    27781 gcggaagcct tctttctgga aacgtgcttc gtcgtagtcg gcgaatttca tcggcacttt
    27841 gtcgaagtag cggctttctg ccccttcgat cacctgatta tcattaatac ggaaagagag
    27901 cagcaccgct tttttcaacc actgatgcgt cacccactga ccgtcaattt tttccgctac
    27961 acgcagtgcg ccggaatcca gcagggcgat cacctgattt accgcttcgc gggtaacggt
    28021 gtctgcattg gctggcgtga tctcggcacg gcgttcaaaa gcggtttcaa taatgttctg
    28081 taactgctgc attgttaaac tcttttcata tcagtaaaca catcaccctt tatcgtttgg
    28141 attgagggcc tctgtcaacc gctgatgcac ttcctgctgc aactcgttat taagcgcacg
    28201 ccggtcagcg gtggcaatta tgaataaatc ttctactcgc tcgccaatgg ttgtaattcg
    28261 ggcaccatga agcgaaattc ccagatcggc aaaaattttc ccgactcgcg ccagcagtcc
    28321 aggttggtcg agggcgatca gttcgaggaa cgatttgcgg tcggtatggg tcggcaaaaa
    28381 cgttacttcg gtttcaacag taaaatggcg taatttggcg ggttggcgac ggggctgcgg
    28441 tggctgccag ctactttgcg tcagcacctg ctccagacca aaccgaataa cctcatggcg
    28501 atctgcggac agcgggctgc catcgggttc cagcacgata aaggtatcca tcgccatacc
    28561 gtcgcgagtg gtgaaaattt gtgcgtcgtg aacacttaaa ttgcggcggt ctaattcggc
    28621 acagacggcg gcaaacagat aggggcggtc cgggctccag ataaaaatct cggtgcctcc
    28681 acgcgtagcc tgcgggctaa gcaataccag cggtttgctt aaatcatgct gtaataaatg
    28741 gcgggcatgc caggccagtt gatttgggct atggcggaca aaatagttag cacgacagcg
    28801 tgaccaaatt tggtgcagcg cctcttcgtc gatgttatcc atgcgcagta gtgccagtgc
    28861 ctggagttgg tgatggcgaa cccgttcgcg catatccggc gtgttttgca ttccgcgtcg
    28921 tagctgcttt tcggtggcaa aatagagttc gcgcaacaga ctttgcttcc agctattcca
    28981 cagtgtttcg ttggtggcgc aaatgtcagc cactgtcagg cataccagat agcgcagacg
    29041 attttccgtt tgaacttctt cggcaaactg cttgatgact tcagggtcct gaatatcgcg
    29101 gcgttgtgct gtcaccgaca tcagcaagtg ctggcgaacc agccaggcaa ccagctgtgt
    29161 ttcgcgtgag ttcagcccgt ggagttctgc aaaatgcact acatcctgag caccgagaat
    29221 ggagtggtcg ccgccgcgtc ctttggcgat atcgtgaaac agcgcggcga tgaaaatcag
    29281 ctcagttgac ggcaggcgcg gccagacgtc cacacacaac ggatggcgct ggcgcgtttc
    29341 ttcactggca aaactctcca gtttcagcat cacgcggata gtatgttcat ccaccgtgta
    29401 ggcgtggaac agatcaaact gcatctgccc gacgatatgc gaccattgcg gcatatacgc
    29461 gccgagcacg ctatggcgat gcattggcaa tagtccgcgc cgcaccgctc cggggtgacg
    29521 cagaatgctc aaaaacagtt ttcgtgcttg cggaatatta cacagcggtt gttgcagatg
    29581 gcgacgggca tggcgtaact ggcgcagcgt ggtggagtaa atgccggtga tcgcactgtt
    29641 gcgcaccatg gtgtagaaca tacgcaagat ggcttccggc tggcgcataa atagtgtttc
    29701 atcacgcagg tcgattagcg taccgcgtag ctgaaactca tcgtcgagtg gacgtggttt
    29761 ttcgtcggcg ggaagggcga ggatggcttc atcgaacagt tgcagcagca tctggttgag
    29821 ttcactgacg cggcgtgtaa cgcggaagta atccttcatc atccgctcga ccggttcgtt
    29881 gccttcacca ctgtaattca gacgctgagc gacgctaagt tggcgatcga ataacagacg
    29941 attatcgtaa cggctgacga ccagatgcag ggcaaagcga atacgccaca gtatatgcag
    30001 acattcgttt aattccgccc gctccgctga ggttaagaag ccaaacccga ccatttcatc
    30061 cagcgatgtt gcgccaaaat gacggcgggc cacccattgc agagtgtgaa tatcgcgcaa
    30121 gccgccaggg ctacttttga tgtctggttc aaggttgtag ctggtgccat ggtaacgctg
    30181 atggcgctgg ttctgttctt caactttcgc tgcgtagaac ttgtcggaag gccagaatcc
    30241 ttcgctaaaa atatgttttt gcagttcgag gaagagcgca acatcgccaa ttaataagcg
    30301 ggattcgatt aaattggtgg cgacggttaa atccgataac ccttccaaca tgcactcttc
    30361 aagcgtgcgc acactatgac cgacttccag ctttacatcc cagagcagcg ttaacagctc
    30421 gcccactttt tgcgcctgat cgtccgggag ctttttacgg cttaaaatca gtaaatcgat
    30481 gtcagaaagt ggatgcagct cgccacgacc gtagccaccg acggcaacca atgccaggtc
    30541 ggcaatctgg ctgaatcccg cttcaatcca taatcgttgc aggagctggt ctatgaactc
    30601 ggtacgtgcc tcaatcaact gttctgcaga gatcccattg tcaaaggcat cacccagcca
    30661 acgctggaaa gtatcgatat gggcttttat cccacagacg gttaattcat cacggggcca
    30721 ggcgcatgga ttttgcggtt gaccgggcag ggtggagaga gcggtgtttg cgtactgttc
    30781 tggaagggta ttcattgtgc gccacccata agattaaatt atcgcattaa aaaagccggc
    30841 attcgccggc ttcatcttat tcgtcgtgcg agattatcgc cgggatggtg tcatccttgc
    30901 gtagcgtcaa aatttcgcag ccgttatcag tcaccacaat agtatgctca tattgtgcag
    30961 acaagctgcg atctttggtt tttaccgtcc agccatcttt catggtgcgg atctcttttt
    31021 tacccgcgtt gaccattggc tcaatggtga acgtcatccc cggtttcagc acgacgttgg
    31081 tttcacggga gtcatagtgc agcacctgcg gttcttcatg gaagccgcga ccaataccgt
    31141 gtccgcaata ttcacgaacg acggagaagc cttctgcttc gacaaatttc tgaatcgccg
    31201 caccgatttc gcgcagatta atgcctggtt ttaccatgcg tagcgccagg tacaggcttt
    31261 cttgcgtgat gcggcacagg cgttcgccca tgatggtcgg cttaccgacg ataaacattt
    31321 tcgaggtatc gccgtggaaa ccatctttga ttacggtgac atcaatgtta acgatatcgc
    31381 catctttcag cagcttagca tcgtccggga taccgtggca caccacttca ttaatagaga
    31441 tgcaaacgga tttcggatag ccgtgatagc cgaggcaggc agaaaccgcg tgttgttcat
    31501 taacaatgta atcattacag atgcgatcca gctcgccggt gctgacgccc ggtttaacat
    31561 acggttcgat catctccagc acttcggcag ccagtcggcc agcgacgcgc attttttcga
    31621 tatcttctgg ggtcttgatt gagatagcca ttaattctgt ccatcagcgt cggtgatacc
    31681 gacaatatat atgtaagtgc cgtcaatggt atcacacccg ggcaaattga gaatcattct
    31741 gaatttcgcc aaacgtgcca ctgaaggttt tctataatag aaaattcgac gtctgatgct
    31801 gtacacagcg ccaacaatta ttggtgtcca cgacgtattt gtggtataaa gcgcgccgga
    31861 cttccgatcc atttcgtata cacagactgg acggaagcga caatctcact ttgtgtaaca
    31921 acacacacgt atcggcacat attccggggt gccctttggg gtcggtaata tgggatacgt
    31981 ggaggcataa ccccaacttt tatatagagg ttttaatcat ggcaactgtt tccatgcgcg
    32041 acatgctcaa ggctggtgtt cacttcggtc accagacccg ttactggaac ccgaaaatga
    32101 agccgttcat cttcggtgcg cgtaacaaag ttcacatcat caaccttgag aaaactgtac
    32161 cgatgttcaa cgaagctctg gctgaactga acaagattgt ttctcgcaaa ggtaaaatcc
    32221 ttttcgttgg tactaaacgc gctgcaagcg aagcggtgaa agacgctgct ctgagctgcg
    32281 accagttctt cgtgaaccat cgctggctgg gcggtatgct gactaactgg aaaaccgttc
    32341 gtcagtccat caaacgtctg aaagacctgg aaactcagtc tcaggacggt actttcgaca
    32401 agctgaccaa gaaagaagcg ctgatgcgca ctcgtgagct ggagaaactg gaaaacagcc
    32461 tgggcggtat caaagacatg ggcggtctgc cggacgctct gtttgtaatc gatgctgacc
    32521 acgaacacat tgctatcaaa gaagcaaaca acttgggtat tccggtattt gctatcgttg
    32581 ataccaactc tgatccggac ggtgttgact tcgttatccc gggtaacgac gacgcaatcc
    32641 gtgctgtgac cctgtacctg ggcgctgttg ctgcaaccgt acgtgaaggc cgttctcagg
    32701 atctggcttc ccaggcggaa gaaagcttcg tagaagctga gtaataaggc ttgataactc
    32761 ccccaaaata gttcgagttg cagaaaggcg gcaagctcga gaattcccgg gagcttacat
    32821 cagtaagtga ccgggatgag cgagcgaaga caacgcatct gcggcgcgaa atatgaaggg
    32881 ggagagccct tatagaccag gtagtacacg tttggttagg gggcctgcat atggccccct
    32941 ttttcacttt tatatctgtg tggtttaatg ccgggcagat cacatctccg aggattttag
    33001 aatggctgaa attaccgcat ccctggtaaa agagctgcgt gagcgtactg gcgcaggcat
    33061 gatggattgc aaaaaagcac tgactgaagc taacggcgac atcgagctgg caatcgaaaa
    33121 catgcgtaag tccggtgcta ttaaagcagc gaaaaaagca ggcaacgttg ctgctgacgg
    33181 cgtgatcaaa accaaaatcg acggcaacta cggcatcatt ctggaagtta actgccagac
    33241 tgacttcgtt gcaaaagacg ctggtttcca ggcgttcgca gacaaagttc tggacgcagc
    33301 tgttgctggc aaaatcactg acgttgaagt tctgaaagca cagttcgaag aagaacgtgt
    33361 tgcgctggta gcgaaaattg gtgaaaacat caacattcgc cgcgttgctg cgctggaagg
    33421 cgacgttctg ggttcttatc agcacggtgc gcgtatcggc gttctggttg ctgctaaagg
    33481 cgctgacgaa gagctggtta aacacatcgc tatgcacgtt gctgcaagca agccagaatt
    33541 catcaaacca gaagacgtat ccgctgaagt ggtagaaaaa gaataccagg tacagctgga
    33601 tatcgcgatg cagtctggta agccgaaaga aatcgcagag aaaatggttg aaggccgcat
    33661 gaagaaattc accggcgaag tttctctgac cggtcagccg ttcgttatgg aaccaagcaa
    33721 aactgttggt cagctgctga aagagcataa cgctgaagtg actggcttca tccgtttcga
    33781 agtgggtgaa ggcatcgaga aagttgagac tgactttgca gcagaagttg ctgcgatgtc
    33841 caagcagtct taattatcaa aaaggagccg cctgagggcg gcttcttttt gtgcccatct
    33901 tgtaaattca gctaaccctt gtggggctgc gctgaaaagc gacgtacaat gtcgctagta
    33961 ttaattcatt tcaatcgttg acagtctcag gaaagaaaca tggctaccaa tgcaaaaccc
    34021 gtctataaac gcattctgct taagttgagt ggcgaagctc tgcagggcac tgaaggcttc
    34081 ggtattgatg caagcatact ggatcgtatg gctcaggaaa tcaaagaact ggttgaactg
    34141 ggtattcagg ttggtgtggt gattggtggg ggtaacctgt tccgtggcgc tggtctggcg
    34201 aaagcgggta tgaaccgcgt tgtgggcgac cacatgggga tgctggcgac cgtaatgaac
    34261 ggcttggcaa tgcgtgatgc actgcaccgc gcctatgtga acgctcgtct gatgtccgct
    34321 attccattga atggcgtgtg cgatagctac agctgggcag aagctatcag cctgttgcgc
    34381 aacaaccgtg tggtgatcct ctccgccggt acaggtaacc cgttctttac caccgactca
    34441 gcagcttgcc tgcgtggtat cgaaattgaa gccgatgtgg tgctgaaagc aaccaaagtt
    34501 gacggcgtgt ttaccgctga tccggcgaaa gattcaaccg caaccatgta cgagcaactg
    34561 acttacagcg aagtgctgga aaaagagctg aaagtcatgg acctggcggc cttcacgctg
    34621 gctcgtgacc ataaattacc gattcgtgtt ttcaatatga acaaaccggg tgcgctgcgc
    34681 cgtgtggtaa tgggtgaaaa agaagggact ttaatcacgg aataattccc gtgatggata
    34741 aataagggta agattccgcg taagtatcgc gggggcgtaa gtctggttat aaggcgttat
    34801 tgttgcaggc agtttggtca cggccagcgc gcagcaaccg gagcgtacaa aagtacgtga
    34861 ggatggcgag cactgctcgg ggccaaaatg gcaaataaaa tagcctaata atccagacga
    34921 ttacccgtaa tatgtttaat cagggctata cttagcacac ttccactgtg tgtgactgtc
    34981 tggtctgact gagacaagtt ttcaaggatt cgtaacgtga ttagcgatat cagaaaagat
    35041 gctgaagtac gcatggacaa atgcgtagaa gcgttcaaaa cccaaatcag caaaatacgc
    35101 acgggtcgtg cttctcccag cctgctggat ggcattgtcg tggaatatta cggcacgccg
    35161 acgccgctgc gtcagctggc aagcgtaacg gtagaagatt cccgtactct gaaaatcaac
    35221 gtgtttgatc gttcaatgtc tccggccgtt gaaaaagcga ttatggcctc cgatctcggc
    35281 ctgaacccga actctgcggg tagcgacatc cgtgttccgc tgccgccgct gacggaagaa
    35341 cgtcgtaaag atctgaccaa aatcgttcgt ggtgaagcag aacaagcgcg tgttgcagta
    35401 cgtaatgtgc gtcgtgacgc gaacgacaaa gtgaaagcac tgttgaaaga taaagagatc
    35461 agcgaagacg acgatcgccg ttctcaggac gatgtacaga aactgactga tgctgcaatc
    35521 aagaaaattg aagcggcgct ggcagacaaa gaagcagaac tgatgcagtt ctgatttctt
    35581 gaacgacaaa aacgccgctc agtagatcct tgcggatcgg ctggcggcgt tttgcttttt
    35641 attctgtctc aactctggat gtttcatgaa gcaactcacc attctgggct cgaccggctc
    35701 gattggttgc agcacgctgg acgtggtgcg ccataatccc gaacacttcc gcgtagttgc
    35761 gctggtggca ggcaaaaatg tcactcgcat ggtagaacag tgcctggaat tctctccccg
    35821 ctatgccgta atggacgatg aagcgagtgc gaaacttctt aaaacgatgc tacagcaaca
    35881 ggggagccgc accgaagtct taagtgggca acaagccgct tgcgatatgg cagcgcttga
    35941 ggaggttgat caggtgatgg cagccattgt tggtgctgct gggctgttac ctacgcttgc
    36001 tgcgatccgc gcgggtaaaa ccattttgct ggccaataaa gaatcactgg ttacctgcgg
    36061 acgtctgttt atggacgccg taaagcagag caaagcgcaa ttgttaccgg tcgatagcga
    36121 acataacgct atttttcaga gtttaccgca acctatccag cataatctgg gatacgctga
    36181 ccttgagcaa aatggcgtgg tgtccatttt acttaccggg tctggtggcc ctttccgtga
    36241 gacgccattg cgcgatttgg caacaatgac gcccgatcaa gcttgccgtc atccgaactg
    36301 gtcgatggga cgtaaaattt ctgtcgattc ggctaccatg atgaacaaag gtctggaata
    36361 cattgaagcg cgttggctgt ttaacgccag cgccagccag atggaagtgc tgattcaccc
    36421 gcagtcagtg attcactcaa tggtgcgcta tcaggacggc agtgttctgg cgcagctggg
    36481 ggaaccggat atgcgtacgc caattgccca caccatggca tggccgaatc gcgtgaactc
    36541 tggcgtgaag ccgctcgatt tttgcaaact aagtgcgttg acatttgccg caccggatta
    36601 tgagcgttat ccatgcctga aactggcgat ggaggcgttc gaacaaggcc aggcagcgac
    36661 gacagcattg aatgccgcaa acgaaatcac cgttgctgct tttcttgcgc aacaaatccg
    36721 ctttacggat atcgctgcgt tgaatttatc cgtactggaa aaaatggata tgcgcgaacc
    36781 acaatgtgtg gacgatgtgt tatctgttga tgcgaacgcg agtgaagtcg ccagaaaaga
    36841 ggtgatgcgt ctcgcaagct gaggataatc cggctacaga gagtcgcgct atttgttagc
    36901 gtagggcttc agtgatatag tctgcgccat ctgatcgtaa gtagttggct ttataaggtc
    36961 aggtatgccg tggttttaca cggctttttt ttgtataggc ttcagtattc ctgagtaccg
    37021 taaaccctgt cagggaataa aaaacgcgtg atgttgtctg ctactcaacc acttagcgaa
    37081 aaattgccag cgcatggctg ccgtcatgtt gcgatcatta tggacggcaa tggtcgctgg
    37141 gcaaaaaagc aagggaagat tcgtgccttt gggcataaag ccggggcaaa atccgtccgc
    37201 cgggctgtct cttttgcggc caacaacggt attgaggcgt taacgctgta tgcctttagt
    37261 agtgaaaact ggaaccgacc agcgcaggaa gtcagtgcgt taatggaact gtttgtgtgg
    37321 gcgctcgata gcgaagtaaa aagtctgcac cgacataacg tgcgtctgcg tattattggc
    37381 gataccagtc gttttaactc gcgtttgcaa gaacgtattc gtaaatctga agcgctaaca
    37441 gccggaaata ccggtctgac gctgaatatt gcggcgaact acggtggacg ttgggatata
    37501 gtccagggag tcaggcaact ggctgaaaag gtgcagcaag gaaacctgca accagatcag
    37561 atagatgaag agatgttaaa ccagcatgtc tgtatgcatg aactggcccc tgtagattta
    37621 gtaattagga ctgggggaga gcatcgcatt agtaactttt tgctttggca aattgcctat
    37681 gccgaacttt actttacaga tgttctctgg cccgatttcg atgaacaaga ctttgaaggg
    37741 gcgttaaatg cctttgctaa tcgagagcgt cgtttcggcg gcaccgagcc cggtgatgaa
    37801 acagcctgat gggggtcgct tttgctgaag tatcgcctga tatctgcttt tgtgttaata
    37861 cccgtcgtca tcgcggcgtt gtttctgttg ccgccggtgg ggttcgccat tgtaacgctg
    37921 gtggtctgca tgctggcagc gtgggaatgg ggacagctta gcggttttac cactcgttcg
    37981 cagcgagtat ggttggcggt gttatgcggg ttattgttgg cgctgatgct ttttctgttg
    38041 ccggaatatc accgaaatat tcatcaaccg ctggttgaaa tctcactttg ggcttcgttg
    38101 ggttggtgga ttgtcgcgct attgctggtg ctgttttacc caggttccgc agcaatctgg
    38161 cgtaactcta aaacattgcg ccttattttt ggcgtgctaa ccattgttcc cttcttctgg
    38221 ggcatgctgg cgttacgggc ctggcactat gacgagaatc attacagtgg cgcaatatgg
    38281 ctgctctatg tcatgatcct ggtatggggc gctgactccg gcgcatatat gtttggcaaa
    38341 ttgtttggta aacataagct ggcaccgaag gtttctccgg gtaaaacctg gcaaggcttt
    38401 ataggtggac tcgctactgc agcggtaatc tcatggggtt atggcatgtg ggcgaatctc
    38461 gacgtcgctc ccgtcacctt actcatttgc tctattgtcg cagcgttagc ctcagtgctc
    38521 ggcgatctga ccgagagtat gtttaagcgt gaagcaggaa ttaaggacag cggtcattta
    38581 attccaggac acggtggtat tttagatcgt attgatagcc tgacggctgc ggtaccggtc
    38641 tttgcttgct tgttgttact ggtattcagg acgctttaac ggaaggtaat atgctgagtt
    38701 ttctctggga tttggcttcg ttcatcgttg cactgggtgt acttatcacc gtgcatgaat
    38761 ttggtcattt ctgggttgcc cggcgttgtg gtgttcgcgt tgagcgtttc tcaatagggt
    38821 ttggtaaggc gctctggcgg cgaactgata agctcggcac cgaatatgtt atcgccctga
    38881 tcccgttggg cggttatgtc aaaatgctgg atgagcgcgc agaaccggtc gttccggaac
    38941 tccgccacca tgccttcaat aataaatctg tcggccaacg agcggcgatt attgccgcag
    39001 gtccggttgc aaacttcatt tttgctatct ttgcctactg gctggttttt attattggtg
    39061 tgcctggcgt acgtccggtg gttggcgaaa tagcagccaa ttcgatagct gcggaagcac
    39121 aaattgcacc aggtacggaa ctaaaagccg tagatggtat cgaaacgcct gattgggatg
    39181 ccgtgcgttt gcagttggtc gataaaattg gcgatgaaag caccaccatt acagtagcgc
    39241 catttggcag cgaccaacgg cgggatgtaa agctcgattt acgtcactgg gcgtttgagc
    39301 ctgataaaga agatccggta tcttcgctgg ggattcgtcc tcgtgggccg caaattgaac
    39361 ctgtactgga aaatgtgcag ccaaactcgg cggcaagcaa ggcaggtttg caagcaggcg
    39421 acaggatcgt taaagtcgat ggtcagccct taacgcagtg ggtgaccttt gtgatgcttg
    39481 tccgggataa cccgggtaaa tccttagcgt tagaaatcga aaggcagggg agtcccttgt
    39541 ctttgacatt aatcccgggg agtaaaccgg gtaatggtaa agcgattggt tttgtcggta
    39601 ttgagccgaa agtcattcct ttgccagatg agtataaagt tgtacgccag tatgggccgt
    39661 tcaacgccat cgtcgaagcc acggacaaaa cgtggcagct gatgaagctg acggtcagta
    39721 tgctgggaaa attgatcacc ggtgatgtga aactgaacaa cctcagtggg ccgatctcta
    39781 tcgccaaggg ggctgggatg acagcggaac tcggggtagt ttattacctg ccgtttcttg
    39841 cgcttattag cgtgaactta gggataatta acctgtttcc gttgcccgta cttgacgggg
    39901 ggcatctgct gttccttgcg atcgaaaaga tcaagggcgg accggtatcc gagcgggttc
    39961 aagacttttg ttatcgcatt ggctcgattc tgctggtgct gttaatgggg cttgcacttt
    40021 tcaatgattt ctctcggtta tgagagttag ttaggaagaa cgcataataa cgatggcgat
    40081 gaaaaagttg ctcatagcgt cgctgctgtt tagcagcgcc accgtatacg gtgctgaagg
    40141 gttcgtagtg aaagatattc atttcgaagg ccttcagcgt gtcgccgttg gtgcggccct
    40201 cctcagtatg ccggtgcgta caggcgacac ggttaatgat gaagatatca gtaataccat
    40261 tcgcgctctg tttgctaccg gcaactttga ggatgttcgc gtccttcgtg atggtgatac
    40321 ccttctggtt caggtaaaag aacgtccgac cattgccagc attactttct ccggtaacaa
    40381 atcggtgaaa gatgacatgc tgaagcaaaa cctcgaggct tctggtgtgc gtgtgggcga
    40441 atccctcgat cgcaccacca ttgccgatat cgagaaaggt ctggaagact tctactacag
    40501 cgtcggtaaa tatagcgcca gcgttaaagc tgtcgtgacc ccgctgccgc gcaaccgtgt
    40561 tgacctaaaa ctggtgttcc aggaaggtgt gtcagctgaa atccagcaaa ttaacattgt
    40621 tggtaaccat gctttcacca ccgacgaact gatctctcat ttccaactgc gtgacgaagt
    40681 gccgtggtgg aatgtggtag gcgatcgtaa ataccagaaa cagaaactgg cgggcgatct
    40741 tgaaaccctg cgcagctact atctggaccg cggttatgcc cgtttcaaca tcgactctac
    40801 ccaggtcagt ctgacgccag ataaaaaagg tatttacgtc acggtgaaca tcaccgaagg
    40861 cgatcagtac aagctttctg gcgttgaagt gagcggcaac cttgccgggc actccgctga
    40921 aattgagcag ctgactaaga tcgagccggg cgagctgtat aacggcacta aagtgaccaa
    40981 aatggaagat gacattaaaa agcttctcgg tcgctatggt tatgcctatc cgcgcgtaca
    41041 gtcgatgcct gaaattaacg atgccgacaa aaccgttaaa ttacgcgtga acgttgatgt
    41101 gggtaaccgt ttctacgtgc gtaagatccg ttttgaaggt aacgatacct cgaaagatgc
    41161 cgtcctgcgt cgcgaaatgc gtcagatgga aggtgcatgg ttggggagcg atctggtcga
    41221 tcagggtaag gagcgtctga atcgtctggg cttctttgaa actgtcgata ccgataccca
    41281 acgtgttccg ggtagcccgg accaggttga tgtagtctac aaggtgaaag aacgtaacac
    41341 tggtagcttc aacttcggga ttggttacgg tactgaaagt ggcgtgagct tccaggctgg
    41401 cgtgcaacag gataactggt taggtacagg ttatgctgtt ggtatcaacg ggaccaaaaa
    41461 cgattaccag acctatgctg aactgtcggt aaccaacccg tacttcaccg tagatggcgt
    41521 aagcctcggt ggtcgtctct tctataatga cttccaggca gatgacgccg acctgtccga
    41581 ctataccaac aagagttatg gtacagacgt gacgttgggc ttcccgatta acgaatataa
    41641 ctcgctgcgt gcaggtctgg gttatgtaca taactccctg tccaacatgc agcctcaggt
    41701 tgcgatgtgg cgttatctgt actctatggg tgaacatccg agcacctctg atcaggataa
    41761 cagcttcaaa acggacgact tcacgttcaa ctatggttgg acctataaca agcttgaccg
    41821 tggttacttc ccgacagatg gttcacgtgt caacctgacc ggtaaagtga ccattcctgg
    41881 atcggataac gaatactaca aagtgacgtt agacacggcg acttatgtgc cgatcgatga
    41941 cgatcacaaa tgggttgttc tgggtcgtac ccgctggggt tatggtgatg gtttaggcgg
    42001 caaagagatg ccgttctacg agaacttcta tgccggtggt tccagcaccg tgcgtggctt
    42061 ccagtccaat accattggtc cgaaagcagt ttacttcccg catcaggcca gtaattatga
    42121 tccggactat gattacgaat gtgcgactca ggacggcgcg aaagacctgt gtaaatcgga
    42181 tgatgctgta ggcggtaacg ccatggcggt tgccagcctc gagttcatca ccccgacgcc
    42241 gtttattagc gataagtatg ctaactcggt tcgtacttca ttcttctggg atatgggtac
    42301 cgtttgggat acaaactggg attccagcca atattctgga tatccggact atagtgatcc
    42361 aagcaatatc cgtatgtctg cgggtatcgc attacaatgg atgtccccat tggggccgtt
    42421 ggtgttctcc tacgcccagc cgttcaaaaa gtacgatgga gacaaggcag aacagttcca
    42481 gtttaacatc ggtaaaacct ggtaagtgtt ctccacaaag gaatgtagtg gtagtgtagc
    42541 gatgacttta ggcgatcaat ataagatcgc cgggccacgc aaagaactgc accctccggt
    42601 gcaaatggga tggtaaggag tttattgtga aaaagtggtt attagctgca ggtcttggtt
    42661 tagcactggc aacttctgct caggcggctg acaaaattgc aatcgtcaat atgggcagcc
    42721 tgttccagca ggtagcgcag aaaaccggtg tttctaacac gctggaaaat gagttcaaag
    42781 gccgtgccag cgaactgcag cgtatggaaa ccgatctgca ggctaaaatg aaaaaactgc
    42841 agtccatgaa agcgggcagc gatcgcacca aactggaaaa agacgtgatg gctcagcgcc
    42901 agacttttgc tcagaaagcg caggcttttg agcaggatcg cgcacgtcgt tccaacgaag
    42961 aacgcggcaa actggttact cgtatccaga ctgctgtgaa atccgttgcc aacagccagg
    43021 atatcgatct ggttgttgat gcaaacgccg ttgcttacaa cagcagcgat gtaaaagaca
    43081 tcactgccga cgtactgaaa caggttaaat aagtaatgcc ttcaattcga ctggctgatt
    43141 tagcgcagca gttggatgca gaactacacg gtgatggcga tatcgtcatc accggcgttg
    43201 cgtccatgca atctgcacaa acaggtcaca ttacgttcat ggttaaccca aaataccgtg
    43261 agcatttagg cttgtgccag gcgtccgcgg ttgtcatgac ccaggacgat cttcctttcg
    43321 cgaaaagtgc cgcgctggta gtgaagaatc cctacctgac ttacgcgcgc atggcgcaaa
    43381 ttttagatac cacgccgcag cccgcgcaga acattgcacc cagtgcggtg atcgacgcga
    43441 cggcgaagct gggtaacaac gtatcgattg gcgctaacgc ggtgattgag tccggcgttg
    43501 aactgggcga taacgtgatt atcggtgccg gttgcttcgt aggtaaaaac agcaaaatcg
    43561 gtgcaggttc gcgtctctgg gcgaacgtaa ccatttacca tgagatccag atcggtcaga
    43621 attgcctgat ccagtccgga acagtggtag gcgcagacgg ctttggttat gccaacgatc
    43681 gtggtaactg ggtgaagatc ccacagattg gtcgcgtaat tattggcgat cgcgtggaga
    43741 tcggtgcctg cacaaccatc gatcgcggcg cgctggatga cactgttatt ggcaatggcg
    43801 tgatcattga taaccagtgc cagattgcac ataacgtcgt gattggcgac aatacggcgg
    43861 ttgccggtgg cgtcattatg gcgggcagcc tgaaaattgg tcgttactgc atgatcggcg
    43921 gagccagcgt aatcaacggg catatggaaa tatgcgacaa agtgacggtt acgggcatgg
    43981 gtatggtgat gcgtcccatc actgaaccag gcgtctattc ctcaggcatt ccgctgcaac
    44041 ccaacaaagt ctggcgcaaa accgctgcac tggtgatgaa cattgatgac atgagcaagc
    44101 gtctgaaatc gcttgagcgc aaggttaatc aacaagacta acgttccatc ttttgttcgc
    44161 caaactttac ggcctgtctc attcttacga ttgcggcagg ccgtgttatt attgtcgttt
    44221 cttatatttt gacaggaaga gtatcttgac tactaacact catactctgc agattgaaga
    44281 gattttagaa cttctgccgc accgtttccc gttcttactg gtggatcgcg tgctggattt
    44341 tgaagaaggt cgttttctgc gcgcagtaaa aaatgtctct gtcaatgagc cattcttcca
    44401 gggccatttc cctggaaaac cgattttccc gggtgtgctg attctggaag caatggcaca
    44461 ggcaacaggt attctggcgt ttaaaagcgt aggaaaactg gaaccgggtg agctgtacta
    44521 cttcgctggt attgacgaag cgcgcttcaa gcgcccggtc gtgcctggcg atcaaatgat
    44581 catggaagtc actttcgaaa aaacgcgccg cggcctgacc cgttttaaag gggttgctct
    44641 ggtcgatggt aaagtagttt gcgaagcaac gatgatgtgt gctcgtagcc gggaggcctg
    44701 atacgtgatt gataaatccg cctttgtgca tccaaccgcc attgtggaag agggcgcgtc
    44761 aattggcgcg aacgcacaca ttggtccttt ttgtatcgtt ggaccccatg tcgaaattgg
    44821 tgagggtacc gtactgaaat ctcacgttgt cgtgaatggt catactaaaa ttggccgcga
    44881 taatgagatt tatcagtttg cctccatcgg cgaagttaac caggatttga aatatgctgg
    44941 cgaaccgacc cgtgtggaaa tcggcgatcg taaccgcatt cgcgaaagcg tcaccattca
    45001 tcgtggcaca gtccagggcg gtggattgac gaaggtgggc agcgacaact tactgatgat
    45061 caacgcgcac attgcgcacg attgtacggt aggtaaccgc tgtattctcg ccaacaacgc
    45121 aacgctggcg ggtcacgtat cggttgacga cttcgcgatc atcggcggca tgaccgcagt
    45181 ccatcagttc tgcatcattg gtgcgcatgt gatggttggc ggctgctccg gtgtggcgca
    45241 ggacgtccct ccttatgtca ttgcgcaggg taaccacgca acgccgttcg gtgtcaatat
    45301 cgaagggctg aagcgccgcg gattcagccg tgaggcgatt accgctatcc gcaatgcgta
    45361 taagctaatt tatcgtagcg gtaaaacgct cgatgaagtg aaaccggaaa ttgctgaact
    45421 ggcggaaaca tatccggaag tgaaagcctt taccgatttc tttgcacgct caacgcgcgg
    45481 tctgattcgt taatgactga ccagcgtcca ttaacgattg ccctggtcgc cggagaaacc
    45541 tccggcgata tcctgggggc cggtttaatc cgcgctctga aagaacgtgt gcccaacgcc
    45601 cgctttgttg gtgttgccgg gccacgaatg caggctgaag gctgcgaagc ctggtacgaa
    45661 atggaagaac tggcggtgat gggcattgtt gaagtgctcg gtcgtctgcg tcgcttactg
    45721 catattcgtg ccgatctgac aaagcgtttt ggcgaactga agccagatgt ttttgttggt
    45781 attgatgcgc ccgacttcaa tattactctt gaaggtaacc tcaaaaagca gggtatcaaa
    45841 accattcatt acgtcagtcc atccgtctgg gcgtggcgac agaaacgcgt tttcaaaata
    45901 ggcagagcca ccgatctggt gctcgcattt ctgcctttcg aaaaagcgtt ttatgacaaa
    45961 tacaatgtac cgtgccgctt tatcggtcat accatggctg atgccatgcc gttagatcca
    46021 gataaaaatg gtgcccgtga tgtgctgggg atcccttacg atgcccactg tctggcattg
    46081 ttgccgggca gccgtggtgc agaagtcgaa atgcttagtg ccgatttctt gaaaactgcc
    46141 cagcttttgc gccagacata tccggatctc gaaatcgtgg tgccgctggt gaatgccaaa
    46201 cgccgcgagc agtttgaacg catcaaagct gaagtcgcgc cagacctgtc ggttcatttg
    46261 ctggatggaa tgggccgtga ggcgatggtc gccagcgatg cggcactact ggcgtcgggg
    46321 acggcagccc tggagtgtat gctggcgaaa tgcccgatgg tggtgggata tcgcatgaag
    46381 ccttttacct tctggttggc gaagcggctg gtgaaaactg attatgtctc gctgccaaat
    46441 ctgctggcgg gcagagagtt agtcaaagag ttattgcagg aagagtgtga gccgcaaaaa
    46501 ctggctgcgg cgctgttacc gctgttggcg aacgggaaaa ccagccacgc gatgcacgat
    46561 accttccgtg aactgcatca gcagatccgc tgcaatgccg atgagcaggc ggcacaagcc
    46621 gttctggagt tagcacaatg atcgaatttg tttatccgca cacgcagctg gttgcgggtg
    46681 tggatgaagt cggacgcggg ccgttagttg gcgcggtcgt caccgctgcg gtgatccttg
    46741 acccggcgcg cccgattgcc gggctgaatg attccaaaaa gctgagcgaa aaacgccgtc
    46801 tggcgctcta tgaagagatc aaagagaaag cgttgagctg gagtctgggc cgcgcggaac
    46861 cccacgaaat cgacgagctg aacattcttc atgcgaccat gctggcgatg cagcgtgccg
    46921 tcgctgggct gcatattgcg ccggaatatg tgttgattga tggtaaccgc tgcccgaaat
    46981 taccgatgcc tgcgatggct gtggtgaaag gggatagccg cgtaccagaa atcagtgccg
    47041 cgtctatcct ggcgaaagtg acgcgtgacg ccgaaatggc ggcgctggat attgttttcc
    47101 cgcaatatgg ttttgcccaa cataaagggt atccaaccgc ttttcatctg gaaaaactgg
    47161 ctgaatatgg cgcgactgaa catcatcgtc gcagctttgg gcctgtcaaa cgcgcactgg
    47221 gacttgcgtc ctgattcttg tgtcgagatt aagtaaaccg gaatctgaag atgtctgaac
    47281 cacgtttcgt acacctgcgg gtgcacagcg actactcgat gatcgatggc ttggccaaaa
    47341 ccgcaccgct ggtaaaaaag gcggcggcgt tgggtatgcc agcactggcg atcaccgatt
    47401 tcaccaacct ttgcggtctg gtgaagttct acggagcggg acatggcgca gggattaagc
    47461 ctatcgtcgg ggcagatttt aatgtccagt gcgacctgct gggtgatgag ttaacccacc
    47521 tgacggtact ggcggcgaac aataccggct atcagaatct ggcgttgctg atctcaaaag
    47581 cgtatcagcg cgggtacggt gccgccgggc cgatcatcga tcgcgactgg cttatcgaat
    47641 taaacgaagg gttgatcctt ctttccggcg ggcgcatggg cgacgtcgga cgcagtcttt
    47701 tgcgtggtaa cagcgcgctg gtagatgagt gtgtcgcgtt ttatgaagaa cacttcccgg
    47761 atcgctattt tctcgagctg atccgcaccg gcaggccgga tgaagaaagc tatctgcacg
    47821 cggcggtgga actggcggaa gcgcgcggtt tgcccgtcgt ggcgactaac gatgtgcgct
    47881 ttatcgacag cagcgacttt gacgcacacg aaatccgcgt cgcgatccac gacggcttta
    47941 ccctcgacga tcctaaacgc ccgcgtaact attcgccgca gcaatatatg cgtagcgaag
    48001 aggagatgtg tgagctgttt gccgacatcc cagaagccct tgccaacacc gttgagatcg
    48061 ccaaacgctg taacgtaact atgcgtcttg gtgaatactt cctgccgcag ttcccgaccg
    48121 gggacatgag caccgaagat tatctggtca agcgtgcaaa agaaggcctg gaagagcgtc
    48181 tggcattttt attccctgac gaggaagaac gtcttaaacg ccgcccggaa tatgacgaac
    48241 gtctggagac tgaacttcag gttatcaacc agatgggctt cccgggctac ttcctcatcg
    48301 ttatggaatt tatccagtgg tcgaaagata acggcgtacc ggtagggcca ggccgtggct
    48361 ccggtgcggg ttcactggtg gcctacgcgc tgaaaatcac cgacctcgat ccgctggaat
    48421 ttgacctgct gttcgaacgt ttccttaacc cggaacgtgt ctccatgcct gacttcgacg
    48481 ttgacttctg tatggagaaa cgcgatcagg ttatcgaaca cgtggcggac atgtacggtc
    48541 gtgatgcggt atcgcagatt atcaccttcg gtacgatggc ggcgaaagcg gtgatccgcg
    48601 acgtaggccg cgtgctgggg catccgtacg gctttgtcga ccgtatctcg aaactgatcc
    48661 cgcccgatcc ggggatgacg ctggcgaaag cgtttgaagc cgagccgcag ctgccggaaa
    48721 tctacgaagc ggatgaagaa gttaaggcgc tgatcgacat ggcgcgcaaa ctggaagggg
    48781 tcacccgtaa cgccggtaag cacgccggtg gggtggttat cgcgccgacc aaaattaccg
    48841 attttgcgcc gctttattgc gatgaagagg gcaaacatcc ggtcacccag tttgataaaa
    48901 gcgacgttga atacgccgga ctggtgaagt tcgacttcct tggtttgcgt acgctcacca
    48961 tcatcaactg ggcgctggag atgatcaaca agcggcgggc gaagaatggc gagccaccgc
    49021 tggatatcgc cgcgatcccg ctggatgaca agaaaagctt cgacatgctg caacgctcgg
    49081 aaaccacggc ggtattccag cttgaatcgc gcggcatgaa ggacctgatc aagcgtctgc
    49141 aacctgactg cttcgaagat atgatcgcac tggtggcact gttccgccct ggtccgttgc
    49201 agtcagggat ggtggataac tttatcgacc gtaaacatgg tcgtgaagag atctcctatc
    49261 cggacgtgca gtggcagcat gaaagcctga aatcggtact ggagccaacc tacggcatta
    49321 tcttgtatca ggaacaggtc atgcagattg cccaggtgct ttctggttat accctcggtg
    49381 gcgcggatat gctgcgccgt gcgatgggta agaaaaagcc ggaagagatg gctaagcagc
    49441 gttctgtatt tgctgaaggt gcagaaaaga acggaatcaa cgccgaactg gcgatgaaaa
    49501 tcttcgacct ggtggagaaa ttcgcgggtt acggatttaa caaatcgcac tctgcggcct
    49561 atgctttggt gtcatatcaa acgttatggc tgaaagcgca ctatccggcg gagtttatgg
    49621 cggcggtaat gaccgccgat atggacaaca ccgagaaggt ggtgggcctg gtggatgagt
    49681 gctggcggat ggggctgaaa atcctgccac cagatataaa ctccggtctt taccatttcc
    49741 acgtcaacga cgacggcgaa atcgtgtatg gtattggcgc catcaaaggg gtaggtgaag
    49801 gtccgattga ggccatcatc gaagcccgta ataaaggcgg ctacttccgc gaactgtttg
    49861 atctctgcgc ccgtaccgac accaaaaagt taaaccggcg ggtgctggaa aaactgatca
    49921 tgtccggggc gtttgaccgt cttgggccac accgcgcggc gctgatgaac tcgctgggtg
    49981 atgcgttaaa agcggcagat caacacgcga aagcggaagc tatcggtcag gccgatatgt
    50041 tcggcgtgct ggccgaagag ccggaacaaa ttgaacaatc ctacgccagc tgccaaccgt
    50101 ggccggagca ggtggtatta gatggggaac gtgaaacgtt aggcctgtac ctgaccggac
    50161 accctatcaa ccagtattta aaagagattg agcgttatgt cggaggcgta aggctgaaag
    50221 acatgcaccc gacagaacgt ggtaaagtca tcacggctgc ggggctcgtt gttgccgcgc
    50281 gggttatggt caccaagcgc ggcaatcgta tcggtatctg cacgctggat gaccgttccg
    50341 ggcggctgga agtgatgttg tttactgacg ccctggataa ataccagcaa ttgctggaaa
    50401 aagaccgcat acttatcgtc agcggacagg tcagctttga tgacttcagc ggtgggctta
    50461 aaatgaccgc tcgcgaagtg atggatattg acgaagcccg ggaaaaatat gctcgcgggc
    50521 ttgctatctc gctgacggac aggcaaattg atgaccagct tttaaaccga ctccgtcagt
    50581 ctctggaacc ccaccgctct gggacaattc cagtacatct ctactatcag agggcggatg
    50641 cacgcgcgcg gttgcgtttt ggcgcgacgt ggcgtgtctc tccgagcgat cgtttattaa
    50701 acgatctccg tggcctcatt ggttcggagc aggtggaact ggagtttgac taatacagga
    50761 atactatgag tctgaatttc cttgattttg aacagccgat tgcagagctg gaagcgaaaa
    50821 tcgattctct gactgcggtt agccgtcagg atgagaaact ggatattaac atcgatgaag
    50881 aagtgcatcg tctgcgtgaa aaaagcgtag aactgacgcg taaaatcttc gccgatctcg
    50941 gtgcatggca gattgcgcaa ctggcacgcc atccgcagcg tccttatacc ctggattacg
    51001 ttcgcctggc atttgatgaa tttgacgaac tggctggcga ccgtgcgtat gcagacgata
    51061 aagctatcgt cggtggtatc gcccgtctcg atggtcgtcc ggtgatgatc attggtcatc
    51121 aaaaaggtcg tgaaaccaaa gaaaaaattc gccgtaactt tggtatgcca gcgccagaag
    51181 gttaccgcaa agcactgcgt ctgatgcaaa tggctgaacg ctttaagatg cctatcatca
    51241 cctttatcga caccccgggg gcttatcctg gcgtgggcgc agaagagcgt ggtcagtctg
    51301 aagccattgc acgcaacctg cgtgaaatgt ctcgccttgg cgtaccggta gtttgtacgg
    51361 ttatcggtga aggtggttct ggcggtgcgc tggcgattgg cgtgggcgat aaagtgaata
    51421 tgctgcaata cagcacctat tccgttatct cgccggaagg ttgtgcgtcc attctgtgga
    51481 agagcgccga caaagcgccg ctggcggctg aagcgatggg tatcatcgct ccgcgtctga
    51541 aagagctgaa actgatcgac tccatcatcc cggaaccgct gggcggcgct caccgtaacc
    51601 cggaagcgat ggcggcatcg ttgaaagcgc aactgctggc ggatctggcc gatctcgatg
    51661 tgttaagcac tgaagattta aaaaatcgtc gttatcagcg cctgatgagc tacggttacg
    51721 cgtaattcgc aaaagttctg aaaaagggtc acttcggtgg ccctttttta tcgccacggt
    51781 ttgagcaggc tatgattaag gaaggatttt ccaggaggaa cacatgaaca tcattgccat
    51841 tatgggaccg catggcgtct tttataaaga tgagcccatc aaagaactgg agtcggcgct
    51901 ggtggcgcaa ggctttcaga ttatctggcc acaaaacagc gttgatttgc tgaaatttat
    51961 cgaacataac cctcgaattt gcggcgtgat ttttgactgg gatgagtaca gtctcgattt
    52021 atgcagcgat atcaatcaac ttaatgaata tctcccgctt tatgccttca tcaacaccca
    52081 ctcgacgatg gatgtcagcg tgcaggatat gcggatggcg ctctggtttt ttgaatatgc
    52141 gctggggcag gcggaagata tcgccattcg tatgcgtcag tacaccaacg aatatcttga
    52201 taacattacg ccgccgttca cgaaagcctt gtttacctac gtcaaagagc ggaagtacac
    52261 cttttgtacg ccggggcata tgggcggcac cgcatatcaa aaaagcccgg ttggctgtct
    52321 gttttatgat tttttcggcg ggaataccct taaggctgat gtctctattt cggtcaccga
    52381 gcttggttcg ttgcttgacc acaccgggcc acatctggaa gcggaagagt acatcgcgcg
    52441 gacttttggc gcggaacaga gttatatcgt taccaacgga acatcgacgt cgaacaaaat
    52501 tgtgggtatg tacgccgcgc catccggcag tacgctgttg atcgaccgca actgccataa
    52561 atcactggcg catctgttga tgatgaacga tgtagtgcca gtctggctga aaccgacgcg
    52621 taatgcgttg gggattctgg gggggatccc gcgccgtgaa tttactcgcg acagcatcga
    52681 agagaaagtc gctgccacta cgcaagcaca atggccggtt catgcggtga tcaccaactc
    52741 cacctatgat ggtttgctct acaacaccga ctggatcaaa cagacgctgg atgtcccgtc
    52801 gatccacttc gattccgcct gggtgccgta tactcatttt catccaatct accagggtaa
    52861 aagtggtatg agcggcgagc gtgttgcggg aaaagttttc ttcgaaacgc aatcgaccca
    52921 caaaatgctg gcggcgttat cgcaggcttc actgatccac attaaaggcg agtatgacga
    52981 agaggcgttt aacgaagcct ttatgatgca taccaccacc tcgcccagtt atcccattgt
    53041 tgcttcggtt gagacggcgg cggcgatgct gcgtggtaat ccgggcaaac ggctgattaa
    53101 ccgttcggtg gagcgggcgc tgcattttcg caaagaggtc cagcggctgc gggaagagtc
    53161 tgacggttgg tttttcgata tctggcaacc gccgcaggtg gatgaagccg aatgcaggcc
    53221 cgttgcgcct ggcgaacaat ggcacggctt taacgatgcg gatgccgatc atatgtttct
    53281 cgatccggtt aaagtcacta ttttgacacc ggggatggac gaacagggca atatgagtga
    53341 ggaggggatc ccggcggcgc tggtagcaaa attcctcgac gaacgtggga tcgtagtaga
    53401 gaaaaccggc ccttataacc tgctgtttct ctttagtatt ggcatcgata aaaccaaagc
    53461 aatgggatta ttgcgtgggt tgacggaatt taagcgctct tacgatctca acctgcggat
    53521 caaaaatatg ctgcccgatc tctatgctga agatcccgat ttctaccaca atatgcgtat
    53581 tcaggatctg gcgcaaggga tccataagct gattcgtaaa cacgatcttt ccggtttgat
    53641 gttgcgggca ttcgatactt tgccggaaat gatcatgacg ccacatcagg catggcagag
    53701 acagattaaa ggtgaagtag aaaccattgc gctggaacaa ctggtcggta gagtatcggc
    53761 aaatatgatc ctgccttatc caccgggcgt accgctgttg atgccaggag aaatgctgac
    53821 caaagagagc cgcacggtac tcgattttct actgatgctt tgttccgtag ggcaacatta
    53881 ccccggtttt gaaacggata ttcacggcgc gaaacaggac gaagacggcg tttaccgcgt
    53941 acgagtccta aaaatggcgg gataacttgc cagagcggct tccgggcgag taacgtgctg
    54001 ttaacaaata aaggagacgt tatgctgggt ttaaaacagg ttcaccatat tgcgattatt
    54061 gcgacggatt atgcggtgag caaagctttc tactgcgata ttcttggttt cacgctgcaa
    54121 agcgaagtct atcgcgaagc gcgcgactcg tggaaagggg atttggcgct taatgggcaa
    54181 tatgtgattg agcttttctc ttttccgttc ccgccggagc gccccagccg accggaagct
    54241 tgcggcctgc gtcatctggc ttttagcgtt gatgacatcg atgcggcagt ggcgcacctt
    54301 gaaagccata acgtgaagtg tgaagccatc cgtgtcgatc catacacgca aaaacgcttc
    54361 accttcttta acgatccgga cgggctgccg ttggaactgt atgagcagta aggcttgtca
    54421 tcgccgcatt tgcccggtaa cgtgccgggc attgctactg taaaatcgca ccatcatgac
    54481 actcacgctc aatagacaac ttctcacctc acgccagatt ctggtggcct ttagcggcgg
    54541 gcttgactcc accgttctgc tgcatcagtt ggtgcagtgg cggacggaaa atccgggcgt
    54601 cactctgcgc gctattcatg tgcatcacgg tttaagtgcc aatgccgatg cctgggttac
    54661 gcattgcgaa aacgtctgcc aacagtggca ggtgccgctg gtggtcgaac gcgtacaact
    54721 tgcgcaagag gggctgggga ttgaggccca ggcgcggcag gcacgttatc aggcatttgc
    54781 ccgtacccta ttgcccggcg aagtgctggt caccgcgcaa catctcgacg atcaatgtga
    54841 aacctttctg ctggcgctaa aacgcggtag cggccctgcc gggctttcgg ctatggcgga
    54901 agtttcggag tttgctggaa cacggcttat ccgcccgttg cttgcccgca cgcgggggga
    54961 actggagcag tgggcattgg cgcatggttt acgctggatt gaagacgaaa gtaatcagga
    55021 cgactcatac gatcgtaact ttctgcgcct gcgcgtagtg ccgttattgc agcagcgttg
    55081 gccgcatttt gcggaagcaa cggcccgcag cgccacactt tgtgctgaac aagagagtct
    55141 gttggatgaa ctgctggcgg atgatttagc acactgccaa acgtcgcagg ggacgctgca
    55201 gattgcgcca atgctggcga tgagtgatgc ccgtcgcgcg gcgattattc gccgctggct
    55261 ggcagggcag aatgcaccga tgccttcccg cgacgcgctg gtgcgcatct ggcaggaagt
    55321 ggcgctggcg cgggaagatg cttccccctg tttacgtttg ggcgcctttg aaatccgccg
    55381 ctatcagtcg caactgtggt ggattaaatc cgtcaccggg caaagcgaaa ccattgtgct
    55441 gtggcagacg tggctacaac cgctggaatt accggcgggg ctgggaaccg tacagcttac
    55501 tgcgggaggc gatattcgcc ctccgcgtgc agacgaagcg gtcagcgtgc gtttcaaagc
    55561 gccaggattg ctgcatattg tcgggcgcaa cggcggacgt aagctgaaga aaatctggca
    55621 agagttaggc gtgccgccgt ggctacgtga caccacgcca ctgctgtttt atggagaaac
    55681 gctgattgcg gcggcagggg tatttgtgac gcaagaaggt gtggctgaag gtgagaatgg
    55741 cgtaagtttt gtctggcaga aaacgcttag ttaagtgaaa gccggataag acgcatcaaa
    55801 cgtcgcatcc ggcgaaagtc aatcaggatt cgcttaccac caccgtaccg atttcagggt
    55861 ggctaaagct ggtaatttta tccagacgca gctcgcgggt ttcgccagcg gcctcgacga
    55921 ccagatactc cacatttttg cgggagacta aatcactggc ttttgcctgc aatttttcgc
    55981 catctttcag ctcaagtgtc agcattaaat gatgctggca ggcgagctca agattatcgt
    56041 aatcatcaca attaattggt tgatacgtat cattcattga cataatcgct caccagtaag
    56101 tttgccgcag cgtatgctgc tttttccctg acagcctcag aaagggcgtc gtcggcagcc
    56161 atttcattca gcactttcaa aacgcagccc agcgcgtccg gaacgtatcc taagtctccg
    56221 ctggcgattt ccgcgtaccg cttgcgtatt aactcacaat atttttccac atgccctcct
    56281 gtcagcactc tgacttaacc gtggatgcaa gtctaagcct acgaagataa actctgtttc
    56341 gcaaggtgac tataccacac tcatttctgc aatatcagcg ccgcaactgc acgtattccg
    56401 ttacaatggc ctcctgattc gaaaggagtt ttcttatggc gcttaaagcg acaatttata
    56461 aagcgacggt taatgtggcc gatctcgacc gcaaccagtt tctcgatgcc tctctgacgc
    56521 tggcgcgcca cccttcagaa acccaggagc gtatgatgct gcgcttgctg gcgtggctga
    56581 aatatgctga tgaacgtctg caatttaccc gtggtttgtg tgccgatgat gagccggaag
    56641 cgtggctgcg taacgatcac ctgggcattg atttgtggat tgagctgggg ctgccggatg
    56701 agcgacgaat taagaaagcc tgcacccagg ccgcagaagt ggcgctgttt acctataata
    56761 gtcgggcggc gcaaatctgg tggcagcaaa atcagagcaa atgtgtgcag tttgccaatc
    56821 tttccgtctg gtatctggac gatgaacaac tggcgaaagt aagcgccttt gccgatcgta
    56881 ccatgacgct gcaggcaacg attcaggatg gtgttatctg gttatcggat gataagaata
    56941 atctggaagt taacttaacc gcctggcaac aaccttcatg attgtgattt cccgacatgt
    57001 tgctattccc gatggtgagc ttgagatcac cgccattcgt gcgcagggcg ctggcgggca
    57061 gcatgttaat aagacctcaa cggctattca tctgcgtttt gacattcggg cgtccagcct
    57121 gccagagtat tacaaagagc gtctgctcgc cgccagccat catttgatca gcagtgatgg
    57181 cgtgattgtc attaaggcac aggaataccg cagtcaggaa ctgaaccgcg aagccgctct
    57241 ggcccggctg gtggcagtga ttaaagattt aacaaaagaa caaaaagccc gacgacccac
    57301 gcggcccacc cgtgcatcga aagagcgcag gctggcatcg aaagcacaaa aatcaagcgt
    57361 gaaggcgatg cgcggcaaag tacgcagcgg tcgggaataa aaagaaggaa tggatggtga
    57421 aaaaagcgat agtgacagcg atggctgtaa tcagcctctt tactctgatg ggatgtaata
    57481 atcgggccga agtcgatacg ctttctccgg cgcaggctgc cgaactgaaa ccgatgccgc
    57541 aaagttggcg cggcgtgcta ccgtgtgccg attgcgaagg aatcgaaatc tctctgttcc
    57601 tcgaaaaaga cggaacatgg gtgatgaatg agcgttatct cggtgctcgt gaagaacctt
    57661 cctccttcgc ttcctacggt acatgggcgc gaaccgctga caagctggta ttaaccgaca
    57721 gcaaaggtga aaagtcatat tatcgggcga aaggcgatgc gctggagatg ctcgatcgtg
    57781 aaggcaatcc aattgaatcg cagttcaact atacgctgga accggcacaa tccagtttac
    57841 ctatgacgcc gatgaccctg cgaggcatgt atttttatat ggctgatgcg gcgaccttca
    57901 ctgattgcgc gaccggaaaa cgtttcatgg tagcgaataa cgcagagctg gagcgtagct
    57961 acctggctgc gcgcggtcac agtgaaaaaa cggtgttact gtcagtagaa ggtcacttta
    58021 cgcttgaggc taatccggat actggtgtgc caaccaaagt attagcgccc gatacggcgg
    58081 gtaaatttta cccgaataag gattgcagta gtttggggca gtaaacctgc ttaaggcaga
    58141 aacaaacgca aaattgcctg atgcgctgcg cttatcaggc ctacagcata taatgcaaca
    58201 ttttgaatta gcacgatttt gtaggccgga taaggcgttt acgccgcatc cggcattttg
    58261 tgcacgaggc tggctttatc actgagtcag cccaacaaag cgacccgcct tgatataaat
    58321 cccaggcttc aggtgcccaa catactgtaa ctgcgtttct ggcttaaacg cagagacatc
    58381 accaatacgc atatgcatca gatcggcagg gctaatccag cccgattgcg ccagtgtaag
    58441 tggatgcccg gatttggcaa atgcatcagt gacaaattcc gaacaaaacc acgacttttt
    58501 gtctccttcg cacacactgc ttaactgcgc tttcgccagg tcgctgacgc actgttggcg
    58561 aaaatcctcg gagaacggat tcagtgagca catctggcga gtcaccataa agggaataaa
    58621 ttcgacaatg ccgcgatagt tataaccgct atctttgatt ttattggcaa acgcggtgat
    58681 atctgtggct tgttgcgggg taagatccgg gactcgtaag acgaaaagct tatcactatg
    58741 cttcatcgct tttttaaggg aaacaatctg gacgccagcg cctgtcgctt ctgcaacgtt
    58801 attatcaccc agaaagattg caacgtgact cacagaggaa gtgctgaaga cgcggattcc
    58861 aaatgaggtt accccaaggc ttgaggagaa cagcaaatcg ccgggtttga gatcaggtgc
    58921 cgttatttct tttattgatt gttcggtgaa agagctttga tgctggaatt tgacagccca
    58981 tgtttttgcc tcggcatcta ctgctgtggc tgatgggtct ggctggctga tatcaacggt
    59041 acaagccgaa agtaaaagaa agctggggag aagcaggcgg cagtacgcct ttggtttatc
    59101 cattttatac aatccatgta aaaaagggcc ctgaaattca ggaccctttc tggcatcagc
    59161 ctttaatctg tttcaccaga tattcgacga tgtcaccagt cttaattaac tgtttctcgc
    59221 cgttgcgacg atatttatat tcgatatcgt cgttgtcgag gttacggtcg cccagcacaa
    59281 tagtgtgcgg aataccgatc agttccatat cagcaaacat cacgcccgga cgctctttgc
    59341 ggtcatccag cagcacttcg ataccttgtg cacgcagttc gctgtacagt ttctcagcaa
    59401 gctcttgtac gcggaaggat ttgtgcatgt tcatcggcag aatcgccacc tggaacggcg
    59461 cgatagcgtc aggccatacg atgccgcgtt cgtcgtagtt ctgctcaatc gccgcagcta
    59521 ccacacgcgt taccccgata ccgtagcaac ccatcgtcag gatttggtta cggccatctt
    59581 caccctgtac ggaggctttc agtgcttcgg agtacttggt acccagctgg aagatgtgac
    59641 caacttcgat accacgtttg atcagcagcg taccctggcc atccgggctt ggatcgccag
    59701 ccaccacgtt acggatatct gcaacttccg gggtagcgac atcgcgatcc cagttgatac
    59761 cgaagtagtg tttaccatcg atgttagcac cagcagcgaa atcactcatc gccgcaacgg
    59821 tacggtcaat caccaccgga atcggcatgt ttaccggacc cagtgaaccc ggaccggctt
    59881 taaccacagc acgaatttct tcttcggtcg cgaaagtcag cgggcttgca acctgcggca
    59941 gtttttctgc tttaacttcg ttcagctcgt gatcaccgcg caccagcagc gcaaccagcg
    60001 ggaagctact gccttcaacc gctttaacca gcagagtctt aaccgttttc tcaatcggca
    60061 gattgaactg ttcaaccagt tccgcgatgg ttttcgcgtt cggcgtatca accagcgtca
    60121 tttcctgggt agcagcagcg cgcggttctt tcggcgcgat agcttctgcc agttcaatgt
    60181 tcgctgcata gtcagaggtg tcggagaaga ccacatcgtc ttcaccgctc tgcgccagca
    60241 cctggaattc gtgagaggcg ctgccgccga tagaaccggt gtcggcctgt acggcgcgga
    60301 aatccagccc catgcggctg aagattttgc tgtaggccgc atacattgca tcgtaggttt
    60361 cctgcaggga ttcctgagaa gtatggaaag agtaagcatc tttcatcagg aattcgcggg
    60421 aacgcatgac gccgaaacgc ggacgcactt cgtcgcggaa cttggtctgg atctgataga
    60481 agttcagcgg cagctgtttg taagagctaa gctcgttacg aatcaggtca gtgataactt
    60541 cttcatgagt tgggccgagt acgaacggac gctcgccacg gtcaacaaaa cgcagcagtt
    60601 ccggaccgta ctgttcccaa cgaccactct cttgccacaa atcggctggc tgaaccaccg
    60661 gcatcgacac ctcgatcgca ccggcgttgt tcatctcttc acgcacgatg ttttcgactt
    60721 ttttcagaac gcgcacgccg gtcggcagcc aggtatataa cccggaggcc agcttgcgga
    60781 tcatcccggc gcgcagcatc agctgatggc tgatcacctc ggcgtcggca ggtgtctcct
    60841 tgagagtgga gagcaggtat tggctagtac gcatgttgtt acggttccag ttggaaggta
    60901 gaacaggctc aaggcgagcc tgggacaaaa aaagtgattt agtttaccag tgcaaaagaa
    60961 atgtcaaaag agaagggcgt gaatttaacg cggttccagc gcaaagactt caaaacctgc
    61021 gtcggtgacg cgccagcgaa cgttaaaatc atgtagccag acggcatagg ttttgcccgt
    61081 ttcctcacct ttacgatagg ccgggcgcgg gtcctgcgcc agtacttcgc ggataaacag
    61141 cgttaactgc ggataacgct tctccagcgt caaaagctgc ttttcgacct ctgcggtaaa
    61201 actcaccgcc atctctgcag ctggcgcact ttgcgcatag ctggcactgg catcgggaag
    61261 cgattcggca aaggggagat acggtttgat atccactacc ggcgtaccat cgaccagatc
    61321 cagactgccg agcttcagaa tcacgctgcc tttatggcaa acaacctctt tcagctctac
    61381 cagcgacatg ccaattgggt tagggcggaa agtagagcgt gttgcgaaaa cccccattct
    61441 ggcgttaccg ccgaggcgcg gtggacgcac agtcggacgc cagccgcctt ccatcgtttg
    61501 atgaaagacg aaaaggatcc ataaatggct gaatgcttcc aggccgcgaa cggcgtcggc
    61561 ctggttgtag gaagcaatga gatgcagttc gccgttggcg ctttttacca gacctggctg
    61621 gcgcggaacg gcgaattttt ctttataggg cgagcgaata acgcctattt gctcgaactg
    61681 gaaactgctc atttcgccgt aatgttaagc gcagagccga tacatacagc ctgacgatag
    61741 cagcccggcg taccgctggt gacttcgcag ctatgcagta atacagcatt ggctttcatt
    61801 ttagaggcat tgatttgcat ctgcttacgt gcggttggaa tgctcggcgg agagtcctga
    61861 ttagaggcct ggcaagagtc gccactgact tcaccgagat cgcggaacgg tttgccgact
    61921 aattcttctg cattggtata aattcggacc ggcgtggcgc gcggcgcttt cggttttgca
    61981 ggctccgctt tcggctgggg tgcagtgctt tgaacgggtt cgacagggga tctgcttaac
    62041 atggaacagc cgcttagcat gagtgctact aaacagatcg gtaaagcacg catagtattt
    62101 cctcaatgta tgatcaaaac gtcaatattg aatcaggagc ttgtaaaaat gacaagacgg
    62161 gcaagcgccc gtcctgaatg atattacaaa ttgtggaaac agcctaaaaa ttaccagcct
    62221 ttaacagcgc cgccgttaaa cactttgttt gctgcttcgt aaacttcatc agactgataa
    62281 gcctggacga atttcttcac gttctcggcg tctttgttat cttcacgcgt tacgatcagg
    62341 tttacgtatg gggagtcttt atcttcgaca aagataccgt ctttcgccgg agtcaggcca
    62401 atctggctgg cataggtggt attgataact gccagagcga tttgcgcgtc gtccagagaa
    62461 cgcggcagtt gcggtgcttc cagttcaaca attttcagat tttttgggtt ctcaacaaca
    62521 tcaagaacgg tcggcagcag gccaacgcca tctttcagtt tgatcaagcc cactttttgc
    62581 agcagcagca gtgaacgacc aagattagtt ggatcgtttg gcacggcaac ctgcgaacca
    62641 tcctgcagtt catccagtga tttgattttc ttggagtaac cagcaatcgg ataaacaaaa
    62701 gtgttgccta ctgcgaccag tttgtagcca cgatctttca gttgctgatc aaggtacggt
    62761 ttatgctgga aggcgttggc gtcgatatcg cctttgctca atgcttcgtt tggcagaaca
    62821 tagtcgttga aggttaccag ctcaacgtcc aggccatatt tgtctttcgc aactttctgc
    62881 gcgacttctg ctacctgctg ttcggcacca acaatcacgc cgactttaat gtggtttgga
    62941 tctttttcat cctgaccgca gcctaccagt gccagtgatc caatcagggc tcccactgcc
    63001 gcaaaggttt tgaatttgaa cgccatacct tattccttct tcaattattt atgttgtgtt
    63061 gaacgttact tgcgagtgac agcccggacg atgcggtcgc ctgcgaactg aattaaataa
    63121 accagaatga ccagcaatac cagtaccgta ttcatcaccg tcgcgttgta gccgatgtag
    63181 ccatactgat agccaatctg acctaaacca ccggcaccga ctgcaccacc catcgcggaa
    63241 taaccgacca gggtaatcag ggtgatagtt gccgcattca ccagacccgg caacgcttcc
    63301 ggtaacagca ctttacagac gatctgcatt ggcgtggcac ccattgcgcg ggaagcttca
    63361 attaacccgg ttgggatctc cagcagagcg ttctcgacca tacgggcaat aaacggtgct
    63421 gcaccaacgg ttaacggaac aattgctgcc tgcaaaccaa tcgatgtacc gacaataacg
    63481 cgggtaaacg gaatcatcca taccagcaag ataatgaacg ggatggaacg gaaaatgttc
    63541 acaatcgcag aaacggtacg atacagcttc gcgttagcaa taatttgccc cggacgcgtg
    63601 acataaagca gaacgccaac cggcagacca atcacaaacc caaaaaatcc ggatacgaag
    63661 gtcattgcca gcgtttccca tacgccacga accagcagcc acatcatcgg ctcagacata
    63721 acccagtacc tctactttta catggtgttc ctgcagccag gcaatggcgg cttgcgtatc
    63781 ttgttgtgtg ccgtgcattt cagtcagcat gatgccgaat ttaacgccac cggcgtagtc
    63841 catctgcgcg ctaataatgt tgttgttgac gttgaaacga cgcgcggttt cagaaagcag
    63901 tggggcatcg accgattgac cggtaaactc cagacgcagc atcggcacgc agtcagtaaa
    63961 tggctccgtt tgcagacgtt cctggtaatc ttccgggata tccagatgca gggtcgactg
    64021 aataaacttc tgcgccagcg gcgttttcgg atgcgagaac acttcactta ccgtgtcctg
    64081 ctcgatcagt tcgccattgc tgatgaccgc cacgcaatca caaatacgct tcacaacgtc
    64141 catttcgtgg gtgatcaaca gaatcgtcaa acccagacgg cggttaatgt ctttcagcag
    64201 ttcgagaata gaacgtgtcg ttgccgggtc cagcgcgctg gtggcttcat cacacagcaa
    64261 tactttggga ttgctggcta atgcacgggc aatcgccaca cgttgtttct gcccaccgga
    64321 aagattcgac gggtagctat catgcttatc gccaagacca accaatgaca gcaattccgt
    64381 cacgcgacgt ttgatctcgt ctttcggtgt gttgtccagc tccagcggca gagccacgtt
    64441 gccaaaaaca gtacgcgaag agagcaggtt aaaatgctgg aaaatcatac caatctggcg
    64501 gcgagctttg gtcaactcgg attctgacag cgtggtcaat tcctggccat cgaccagcac
    64561 gctaccctcg gttgggcgct ccagcaggtt tacacaacgt ataagcgtac tcttacccgc
    64621 gcctgaggca ccgataacgc cataaatttg tccagctggc acatgcaggc tgacgttgtt
    64681 caacgcctgg atggtgcggg tgccctggtg gaacactttg gtgatattcg aaagttttat
    64741 cattgattat ttattatcgt cattaagtta gtcgtagcat ctcgaatgcc tgaaacgggc
    64801 aacgccgtca atgaaatgga tgttaaggca tccagacgtc taaatcaatc aggtttatgc
    64861 gaagagcact ttcttgcagg tcgaaacatg cgatactagc gtcccatgcc ttattaagga
    64921 gctataaaag gtggcgaaga gcgtacccgc aatttttctt gaccgtgatg gcaccattaa
    64981 tgtcgatcac ggctatgtcc atgagatcga caactttgaa tttatcgacg gtgttattga
    65041 cgccatgcgc gagctaaaaa aaatgggctt tgcgctggtg gtagtaacca accagtctgg
    65101 cattgctcgc ggtaaattta ccgaagcaca gtttgaaacg ctgaccgagt ggatggactg
    65161 gtcgctggcg gaccgagatg tcgatctgga tggtatctat tattgcccgc atcatccgca
    65221 ggggagtgtt gaagagtttc gccaggtctg cgattgccgc aaaccacatc cggggatgct
    65281 tttgtcagca cgcgattatt tgcatattga tatggccgct tcttatatgg tgggcgataa
    65341 attagaagat atgcaggcag cggttgcggc gaacgtggga acaaaagtgc tggtgcgtac
    65401 gggtaaacct attacacctg aagcagaaaa cgcggcagat tgggtgttaa atagcctggc
    65461 agacctgccg caagcgataa aaaagcagca aaaaccggcg caatgattaa aagatgaacg
    65521 gttgaaataa aaatgcattt ttccgcttgt cttcctgagc cgactcccta taatgcgcct
    65581 ccatcgacac ggcggatgtg aatcacttca cacaaacagc cggttcggtt gaagagaaaa
    65641 aatcctgaaa ttcagggttg actctgaaag aggaaagtgt aatatacgcc acctcgcgac
    65701 agtgagctga aagctgcgtc gcaactgctc tttaacaatt tatcagacaa tctgtgtggg
    65761 cactcgaaga tacggattct taacgtcgca agacgataaa tgaataccaa gtctcaagag
    65821 tgaacacgta attcattacg aagtttaatt ctttgagcat caaactttta aattgaagag
    65881 tttgatcatg gctcagattg aacgctggcg gcaggcctaa cacatgcaag tcgaacggta
    65941 acaggaagcg cttgctgctt cgctgacgag tggcggacgg gtgagtaatg tctgggaaac
    66001 tgcctgatgg agggggataa ctactggaaa cggtagctaa taccgcataa cgtcgcaaga
    66061 ccaaagaggg ggaccttcgg gcctcttgcc atcggatgtg cccagatggg attagcttgt
    66121 tggtggggta acggctcacc
//
LOCUS       DAEEUE010000009        65614 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.009, whole genome shotgun sequence.
ACCESSION   DAEEUE010000009 DAEEUE010000000
VERSION     DAEEUE010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65614
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.009"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="KLK84_000612"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="KLK84_000612"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HBE2008070.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="KLK84_000613"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="KLK84_000613"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBE2008071.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="KLK84_000614"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="KLK84_000614"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBE2008072.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="KLK84_000615"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="KLK84_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HBE2008073.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="KLK84_000616"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="KLK84_000616"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HBE2008074.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFCINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="KLK84_000617"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="KLK84_000617"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBE2008075.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="KLK84_000618"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="KLK84_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HBE2008076.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="KLK84_000619"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="KLK84_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBE2008077.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="KLK84_000620"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="KLK84_000620"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBE2008078.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="KLK84_000621"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="KLK84_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HBE2008079.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="KLK84_000622"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="KLK84_000622"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HBE2008080.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="KLK84_000623"
     CDS             complement(15665..16702)
                     /locus_tag="KLK84_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBE2008081.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..17523
                     /gene="yhhY"
                     /locus_tag="KLK84_000624"
     CDS             17035..17523
                     /gene="yhhY"
                     /locus_tag="KLK84_000624"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBE2008082.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="KLK84_000625"
     CDS             complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="KLK84_000625"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBE2008083.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19444..19884
                     /locus_tag="KLK84_000626"
     CDS             19444..19884
                     /locus_tag="KLK84_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HBE2008084.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDNSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="KLK84_000627"
     CDS             complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="KLK84_000627"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBE2008085.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20611..21601)
                     /locus_tag="KLK84_000628"
                     /pseudo
     CDS             complement(20611..21601)
                     /locus_tag="KLK84_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="KLK84_000629"
     CDS             complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="KLK84_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HBE2008086.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="KLK84_000630"
     CDS             complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="KLK84_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HBE2008087.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVMLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="KLK84_000631"
     CDS             complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="KLK84_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HBE2008088.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25143..25856)
                     /gene="livF"
                     /locus_tag="KLK84_000632"
     CDS             complement(25143..25856)
                     /gene="livF"
                     /locus_tag="KLK84_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HBE2008089.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25858..26625)
                     /gene="livG"
                     /locus_tag="KLK84_000633"
     CDS             complement(25858..26625)
                     /gene="livG"
                     /locus_tag="KLK84_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HBE2008090.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26622..27899)
                     /gene="livM"
                     /locus_tag="KLK84_000634"
     CDS             complement(26622..27899)
                     /gene="livM"
                     /locus_tag="KLK84_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HBE2008091.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27896..28822)
                     /gene="livH"
                     /locus_tag="KLK84_000635"
     CDS             complement(27896..28822)
                     /gene="livH"
                     /locus_tag="KLK84_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HBE2008092.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28870..29979)
                     /gene="livK"
                     /locus_tag="KLK84_000636"
     CDS             complement(28870..29979)
                     /gene="livK"
                     /locus_tag="KLK84_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HBE2008093.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30403..30786
                     /gene="panM"
                     /locus_tag="KLK84_000637"
     CDS             30403..30786
                     /gene="panM"
                     /locus_tag="KLK84_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HBE2008094.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="KLK84_000638"
     CDS             complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="KLK84_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HBE2008095.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEELLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="KLK84_000639"
     CDS             complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="KLK84_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HBE2008096.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="KLK84_000640"
     CDS             complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="KLK84_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HBE2008097.1"
                     /translation="MNKRDAINHIRQFGGRLERFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="KLK84_000641"
     CDS             complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="KLK84_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBE2008098.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="KLK84_000642"
     CDS             complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="KLK84_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBE2008099.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36826..37422
                     /gene="rsmD"
                     /locus_tag="KLK84_000643"
     CDS             36826..37422
                     /gene="rsmD"
                     /locus_tag="KLK84_000643"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HBE2008100.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37412..37681
                     /locus_tag="KLK84_000644"
     CDS             37412..37681
                     /locus_tag="KLK84_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HBE2008101.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37684..38043)
                     /locus_tag="KLK84_000645"
     CDS             complement(37684..38043)
                     /locus_tag="KLK84_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HBE2008102.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38184..38810
                     /locus_tag="KLK84_000646"
     CDS             38184..38810
                     /locus_tag="KLK84_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HBE2008103.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38884..41082
                     /gene="zntA"
                     /locus_tag="KLK84_000647"
     CDS             38884..41082
                     /gene="zntA"
                     /locus_tag="KLK84_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HBE2008104.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="KLK84_000648"
     CDS             complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="KLK84_000648"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HBE2008105.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41650..42315
                     /locus_tag="KLK84_000649"
     CDS             41650..42315
                     /locus_tag="KLK84_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HBE2008106.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42388..42945
                     /gene="dcrB"
                     /locus_tag="KLK84_000650"
     CDS             42388..42945
                     /gene="dcrB"
                     /locus_tag="KLK84_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HBE2008107.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42949..44166)
                     /locus_tag="KLK84_000651"
     CDS             complement(42949..44166)
                     /locus_tag="KLK84_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE2008108.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44300..45349
                     /locus_tag="KLK84_000652"
     CDS             44300..45349
                     /locus_tag="KLK84_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBE2008109.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45404..45991
                     /gene="acpT"
                     /locus_tag="KLK84_000653"
     CDS             45404..45991
                     /gene="acpT"
                     /locus_tag="KLK84_000653"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HBE2008110.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46102..47676
                     /gene="nikA"
                     /locus_tag="KLK84_000654"
     CDS             46102..47676
                     /gene="nikA"
                     /locus_tag="KLK84_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HBE2008111.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47676..48620
                     /gene="nikB"
                     /locus_tag="KLK84_000655"
     CDS             47676..48620
                     /gene="nikB"
                     /locus_tag="KLK84_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HBE2008112.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48617..49450
                     /gene="nikC"
                     /locus_tag="KLK84_000656"
     CDS             48617..49450
                     /gene="nikC"
                     /locus_tag="KLK84_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HBE2008113.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49450..50214
                     /gene="nikD"
                     /locus_tag="KLK84_000657"
     CDS             49450..50214
                     /gene="nikD"
                     /locus_tag="KLK84_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HBE2008114.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50211..51017
                     /gene="nikE"
                     /locus_tag="KLK84_000658"
     CDS             50211..51017
                     /gene="nikE"
                     /locus_tag="KLK84_000658"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HBE2008115.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51023..51424
                     /gene="nikR"
                     /locus_tag="KLK84_000659"
     CDS             51023..51424
                     /gene="nikR"
                     /locus_tag="KLK84_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HBE2008116.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51544..51903)
                     /locus_tag="KLK84_000660"
     CDS             complement(51544..51903)
                     /locus_tag="KLK84_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBE2008117.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51900..52175)
                     /locus_tag="KLK84_000661"
     CDS             complement(51900..52175)
                     /locus_tag="KLK84_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HBE2008118.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KKGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52234..53358)
                     /locus_tag="KLK84_000662"
     CDS             complement(52234..53358)
                     /locus_tag="KLK84_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE2008119.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53358..56093)
                     /gene="rbbA"
                     /locus_tag="KLK84_000663"
     CDS             complement(53358..56093)
                     /gene="rbbA"
                     /locus_tag="KLK84_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HBE2008120.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGLGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56090..57157)
                     /locus_tag="KLK84_000664"
     CDS             complement(56090..57157)
                     /locus_tag="KLK84_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBE2008121.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57523..59145)
                     /locus_tag="KLK84_000665"
     CDS             complement(57523..59145)
                     /locus_tag="KLK84_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HBE2008122.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKDYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTKPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59407..60996)
                     /locus_tag="KLK84_000666"
     CDS             complement(59407..60996)
                     /locus_tag="KLK84_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HBE2008123.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENCFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61381..62412
                     /locus_tag="KLK84_000667"
                     /pseudo
     CDS             61381..62412
                     /locus_tag="KLK84_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62499..62975)
                     /locus_tag="KLK84_000668"
     CDS             complement(62499..62975)
                     /locus_tag="KLK84_000668"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2008124.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(62968..63732)
                     /locus_tag="KLK84_000669"
     CDS             complement(62968..63732)
                     /locus_tag="KLK84_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HBE2008125.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINLPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEIFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            63978..64739
                     /locus_tag="KLK84_000670"
     CDS             63978..64739
                     /locus_tag="KLK84_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBE2008126.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDSFNSKPKKIRFLTDILSPQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(64846..65346)
                     /locus_tag="KLK84_000671"
     CDS             complement(64846..65346)
                     /locus_tag="KLK84_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2008127.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
BASE COUNT        15937 a        17624 c        16160 g        15893 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatga cggcttaaaa tgtcctgaat ggtcgaggaa accaagaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcgca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tacctggctg attaatgcag aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcagc cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tgttacgggt aactgttatc ggcaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctgaat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tggcacaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgacacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    17341 atgattgaaa tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc
    17401 gataacgcgc cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt
    17461 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa
    17521 tagagaattt gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat
    17581 attaataccc cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac
    17641 cgtccggccc aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct
    17701 gaccttttgc ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct
    17761 cgtccggcaa ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc
    17821 cataatcgat gctattcacc accatttgca gcaccgtggt gataatccgg ctaccgcctg
    17881 ggctaccggt aaccagccag gttttaccgt ctttcaccac aatggtcggc gacatcgacg
    17941 acagcgggcg tttgttcggc ccgacggcgt tggcatcacc gcccaccagc ccgtaaacgt
    18001 tcggtacgcc cggtttggcg gagaaatcat ccatctggtt attaagcaga ataccgctct
    18061 cacccgcgac aatacccgta ccgaaggtgg tgttcagcgt ataggtcacc gccaccgcgt
    18121 taccgtcttt atccactaca gagtaatggg tagtttgatt actctcataa ggcgcaagct
    18181 tgccagggcg aatctcactg gacggcttcg cttttttgat atcaatttga tcggcaatgg
    18241 atttggcata ggctttattg gtcagtgcct gccacgggac tttgacaaaa tccgggtcgc
    18301 caagatattc cgagcggtcg gcgtaggcgt atttctccgc ttctgccatg atttgcatcg
    18361 catcggcgct gccaaagccg tatttcttca tatcgaagtt ttccagaata ttgaggattt
    18421 gtacgatatg gatcccgccg gaggatggcg gtggcatgga gtaaacctga tacccgcgat
    18481 aatcgccgct tatcggagtg cgttcgaccg ctttataggc cgctaaatct tctttagtga
    18541 tcaagccacc gtttttctgc atctcctggg cgatctgttc cgcaatcgtg cctttataga
    18601 attcgtccgg gccgttttca gcaatcatct ccaggctctt tgccaggttc gcctgcacca
    18661 gcgtgtcgcc ctttttcagc ggctcgccct ctttccagaa gatagcttta ctgttttcgt
    18721 gattcggcaa cacttcgcta ccgtaggttt tgagatcgtc agccagcgcg tcgttaacga
    18781 taaaaccatc gcgtgccagt ttaaacgcgg gctgcacgac tttgttcagc ggcatggtgc
    18841 cgtatttatc cagcgccagc gagaaacctg ctaccgtacc cggtgtgccg gaagccagat
    18901 gcgaagtgag tgattttttg ctgtccgggt tgccctgatc atcgaggaac atatcgcggg
    18961 tcgctttggc gggtgccatt tcgcggaaat cgatagccgt ggtattgcca tttttcgagc
    19021 ggattaacat aaaaccacca ccgcccagat tccctgcctg cggatgcgtt accgccagcg
    19081 cgtagcccac cgccacggcg gcatcaacgg cattcccgcc ctccttgagg atatccaccc
    19141 ccacctgagt ggcagtggcg tccacagacg ctaccattcc ctgtttcgcg cgtaccggat
    19201 ggaagacatc ttcctccaca ccatacgaga cgggcggcgc aggaggcgcg gcggcggcgc
    19261 taaaacaact tcctgagagc agagcagcaa tggccacccg gcgtaaaaac gtcggtttta
    19321 tcatcgttat tctccagaga ttaaggggca accccagcta agcctggtat ataactctga
    19381 attaatcatc gttttgtcgg gaagcgagta aacttaaagg atatcttcag aggaggatac
    19441 gcgatgaaac gacttctgat tcttacggca ctcctgccgt ttgtcggctt tgcacagccc
    19501 attaatactc tgaacaaccc taaccagccg gggtatcaga tacccagcca gcagcggatg
    19561 caaacccaga tgcagactca gcaaatccag caaaaaggga tgctgaatca gcaactgaaa
    19621 acgcaaactc agttgcaaca gcagcattta gaaaaccaga taaacaataa ttctcagcgg
    19681 gtgttgcagt cacagccggg ggagcgaaat cccgcccggc agcaaatgct gcccaacacc
    19741 aacggcggga tgttaaacag caaccgtaat ccggataatt cgttgaatca gcagcatatg
    19801 ctgccggaga ggagaaacgg cgacatgctg aatcagccca gcacgccgca gcctgacatt
    19861 ccgttgaaaa ctattgggcc gtaaagttcg gaccaatcac gtcaatcgca tcggtacaga
    19921 tgcaatccac gccccagcgc agcaactctg ccgcgtgctg gggtttattg acggtataaa
    19981 ccagaatccg taatccggcg tctttcaact gcatcactcg cgctttatcg agtaacttat
    20041 gattgagatg aatagagacg cagcccagcc gcgcggtcag ttcgcgccag tcgtcgcgcc
    20101 actcatcaag caataaacca cgcggcagtt ccggtgccgc ctgttgtgca gcttctaaag
    20161 catcaatctc aaacgatgac agcagcggcg gcgtcatacc ggcccacagt tggcgtgccg
    20221 ccagcgccac cattttgccc gttaatggtc cagtgccagt ggtgggtttg atttcgatat
    20281 tcgccatcat cccgtgttcg cgacagcgtt ccgccacctg cgaaagcaac ggcagcggct
    20341 cacctttaaa cgctttgctg taccaactgc ccgcatccac gcgcagtaaa tcctgccagt
    20401 tcagttcacc cgcgacgccc cagccgttgc tggtgcgttc gagattgtcg tcatggagca
    20461 ggaagatctc gccatctttc gataacttcg cgtcaaattc gatcatctta tgaccgtatt
    20521 ttgccccgac gtcgattgcc gccagggtgt tttccggggc cagcttaccg ccgccacgat
    20581 gagcgacgat gcggggataa ggccagttac tcatactcgt tgtcctgttt caccatcaaa
    20641 aagatgcagc tgattttccg ccagatgcag ccacagcgtg ctgcctgccg tcgggcgctc
    20701 ctgatgcgcc agtcgcacca ccagcttctg cttgcgagct tagcgcaata tgttccgggc
    20761 gaatgccgag ggtcatttta cgcccggcat actgacggta gccaccgttt agcggtagca
    20821 cgataccgcc gtccagttcg aaatgcgtgc cttcattatt catgcggcct gtcagcaggt
    20881 tcatcgccgg gctgccgata aaactcgcca caaacaggct ggcgggcttt tcgtagactt
    20941 ccaccggcgt gccaatctgt tcggcaacgc cgccgttcat caccattact cgctgggcga
    21001 gcgtcatcgc ctcaacctga tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt
    21061 gcagctgttg cagttcaaga cgcatctgta cgcgcagctt ggcatcgagg ttagagagtg
    21121 gctcatcaaa caagaacacc gccggatcgc gcacaatcgc gcgtcccatc gccacacgct
    21181 ggcgctgacc gccggaaagc tcgcgcgggc gacgtttgag cagaccgtcc agctccagaa
    21241 tgcgcgccgc ttctttaacg cgctcggcta tttgctgctt gcccatgccg cgaattttca
    21301 gcccccacgc catgttttct tcgacgctca tatgcggata aagcgcgtaa ttctggaaca
    21361 ccatcgcaat cccgcgatct ttcggctcca tttcggtcac gcgctggtcg ttgatccaga
    21421 tatcgccttc ggtcaccctc tccagcccgg caaccatacg cagcagcgtc gatttcccgc
    21481 agccagacgg cccgaccatc acgataaatt cgccatccgc cacatcaagg gtcagcggtt
    21541 taatgaccta ggttttgcca tcccagcttt tggttactgc ctgtaatttc agtcctgcca
    21601 tcttatttct cactatcgac caggccgcgc acgaaggcac gctgcatcac taaaacaatc
    21661 actactggtg ggatcagggt taacaacatc gccgccatca ctgagttcca ttccgtggtg
    21721 ccttcgccag tggcgatcat ccctttgatc cctgccacgg tggtgccgag atccacgtcg
    21781 gtaataatca acaacggcca caaatactgg ttccagccgt aaataaaggt gatcacaaac
    21841 agcgccgcca gattagtttt ggagagcgga aaaacgatgt cgcaaaagaa gcgcattggt
    21901 gacgcaccgt cgatccgcgc cgcttccacc agttcatccg gcagcgtcat aaagaactgg
    21961 cgaaacagaa acgtggcggt cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg
    22021 tcgagcatct tcaggttggc gatgacttcc accgtcggga agatacgtac ttcaaccggc
    22081 agcatcaggg tgataaaaat catccagaag aagaggttac gtagcggaaa acgaaaccag
    22141 acaatggcaa atgccgagag catcgagaca gtaattttgc cgagcgtaat gctgaacgcc
    22201 atcacaaagc tgttaagcaa catccgccag aacggcgcgc tatttgtgcc taccccgttc
    22261 acccagatgt tgtggatgtt ttccagcaga tgcgtgccgg ggatgagcgt catcggcgcg
    22321 gcgtagacgg cctgtttatc cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg
    22381 atcaccgcga tcccgaggat cagcatggta tggctgaata tcgtcagcca cggacggttc
    22441 tcaatcattg gtaacgcacc ttgctttcaa catagcggaa ctgcaccacc gtcagcacga
    22501 tgacgaggaa catcaacacc atcgactgtg cggcagacga agccagatcc agtccggtaa
    22561 aaccttcgcg gtagatctta taaatcagcg tcgtggtggc ctgaaccggc ccgccggacg
    22621 tggcggcgtc gatcaccggg aaggtgtcga agaaggcata caccaggttc actaccagca
    22681 ggaagaaact caccggggcg ataagcggca gcgcaatctt aaagaagcgg cgaatcggcc
    22741 ctgcaccgtc gatggctgcg gcttcgatca acgaacgggg aatggattgc agcgcggcat
    22801 agaagaacag aaagttgtag ctgatttgct tccacaccga ggcaaacacc accagaaaca
    22861 tcgcctgacc gctattttgc gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg
    22921 tgatcagccc gcgaccgggg ttaaacagga agatccacaa tacggcggca acggcgggag
    22981 ccacggcata cggcagcaac attaaggttt ggtagaaacg actgccgcgc acgatgtact
    23041 ccaccagcgc cgcaaagaac agcgatacca gcaaaccgct gacggtgaca aaggtgctga
    23101 attttatcgt cgtccagaag gagtcgagat aatagctgtc atgaaacagc atgacgaagt
    23161 tatccagacc gacaaactgg ctggagaaac caaacggatc gacgctttgt agcgagtacc
    23221 acaacgcttc gcccgcaggc cagataaaaa agataacggt gatgatgagc tgcggcgcga
    23281 ccagcagata aggcagccag cgcgagcgga acaccggacg ggatgatgac attacggtta
    23341 attcctgaac tgtgccggat gcgcttcgct tatccggcct acacgcgatg ccaggtcaga
    23401 taagcgaaac gcatccggca ttacactgat taagacttcg tcgatttctc aaagcggcgc
    23461 agtaactggt tcccacgctc aactgcggta tccagcgcct gctgtggcgt cttcttaccg
    23521 gtccacacgc tctcaagctc ttcatccaca atcacgcgga tctgcggcat gttgcccaga
    23581 cgaagccctt tggtgaacgg caacggcggc ttgttcagca tctgacgcgt cgcggtatcc
    23641 gcacctgggt ttttctcgta aaagccctgc tcacgggtca ggtcatacgc cgctttggta
    23701 attggcagat aaccggtttt ctgatgccac tcagcagcgt tttctggctt cgcgaggaag
    23761 tcgagaaact tcgccacgcc ggtatacgtt tctttatctt tgccctgcat cacccacagg
    23821 ctggctccgc cgataatggc gttttgtggc gcatctttcg catcggcgtc gtaaggcatc
    23881 atacctacgc cgtagttaaa tttggcgtac tcgcgaatgt tggcaagaga accggaagag
    23941 gcggtggtca tcgcgcaatc accgttatag aacttctcgg tggattcatc cttacgaccg
    24001 acgtagctga agtcgccctt cttgttcatc tcctcgagca tggcgatgtg tttcacctgc
    24061 tccggcttgt tgaactccag caccgcgtcc gtgccgtcaa agccgttgtt tttgctggca
    24121 aacggcagac cgttccaggc gctaaagttt tccagttgga tccagccctg ccagccgctg
    24181 gcgtagccgc acttaatacc ggaggctttc agttttgcgg aatagtccgc cagatcctgc
    24241 caggttttcg gcggctgttc cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa
    24301 tagagaacgg gagtcgagct gttgaacggc tgggagagta agtggccggt tttgctgtcg
    24361 gagtagtaac cggaaaccgt cggcacaaac tgcgactcat cgaactgaat ccctgcctct
    24421 ttaaacacgt catacaccgg tttaatggct ttcgacgcca tcatggtggc ggtgccaact
    24481 tcataaacct gcaaaatagc cggagcgttg ccggtacgaa atacggcaat ccccgcgctt
    24541 aaattctgtt cgtagttgcc tttataggtc ggtacaattt tgtaatccgg attttcggcg
    24601 ttaaaacgtt gggctagaga atccacctct ttacccagtt ccccttccat agaatgccag
    24661 aacggaatgg tcgtcactgc ctgtgcattc cccattaacg ccagtccgag cgccagtgct
    24721 gaagctgtat aatgtaacgg tttcatcgtt tatctctctt gttgtaccga atgcgcgaat
    24781 tcacgcgttt tatgctcgcg gggtaacatg acatgctcga attacagaaa aataactttt
    24841 ttgttacatt tgtaagatag taaggtgtca gaaagatgac aaggcggtga cggcgtgggt
    24901 gagggaaaat gggagatggg gcacggataa gcggaaaaat agaaggtctg aatcaaactc
    24961 tacagattac tcatcgtttc atgccggatg cggcgtaaac gccttatccg gcctacaaga
    25021 tcgtgcaaat tcaatatatt gcaactcacc cagtaggcct gataagcgca gcgcatcagg
    25081 caattttgca tttgtcacct gtctcaaagg agtcttttga ctccttatca atcaacgtgt
    25141 tattacccgc ctaaatacgc acttctcact gcttcgttcg ccagcagcgc gtcgccggta
    25201 tcggagagca ccacatggcc gttttccagc acgtagccgc gatccgccag ctttagcgcc
    25261 tggttggcgt tctgctcgac gaggaagata gtcatcccct gctcgcgcag ctgctcgatg
    25321 gtgtcgaaaa tttgctggat gataatcggc gcaagaccga gcgatggctc atcaagtaac
    25381 aacaaacgcg gattgctcat cagcgcgcga ccaatcgcca gcatctgctg ttcaccgccg
    25441 gacatggtgc ccgcccgctg aacacggcgc tcatgcagac gtggaaacag ttcatacacc
    25501 cactttatgc gctcctgaaa ctggtcacgt tcggcaaaga agccgcccat cgccaggttc
    25561 tcttccaccg tcatccgcga gaagacgcga cgcccttccg ggacaatcgc taccgcttcg
    25621 cgcatgattt tcgccgtctg ccagtcggta atgtctttat catcaaacac aattcgcccg
    25681 ctggtggcgc gcggatcgcc gcataacgtg ccgagcaagg tggttttccc cgccccgttc
    25741 gcgccaatga gagtgacaat ctcgccctga ttgatatgca ggctcacctc atgcagcgcc
    25801 tggattttgc cgtagtgggc gctgactttg tcaaaggaca acatgacttt ttccatctta
    25861 tgcctcacct aaataggcac ggatcacgtc cgggttatta cggatttgct ccggcgtacc
    25921 gtttgccagt ggcgtcccct gattgaccac ataaattcgg tctgaaatcc ccatcaccag
    25981 cttcatatcg tgttcaatca acaagatagt ggtgttgtga tgattgcgca gttcggcaat
    26041 cagctcatcc agctctttgg tttcttttgg gttaagacct gccgccggtt cgtcgagcat
    26101 taaaatctcc ggctgcgtca ccatgcagcg ggcaatctcc agacggcgtt ggtcaccata
    26161 ggccaggtta ctcgcctgac ggttggcgtg ttccagcaaa ccaatgcgct caagccaggt
    26221 ggcggcgcgg tcgagcgctt cgctctgggc gcgacggaaa gaaggcgttt tcaacaggcc
    26281 agagaacagc ccggttttca gttgctgatg ctgcgccacc agcaggtttt caattaccgt
    26341 catttcacgg aacagacgca catgctggaa ggtgcgcacc acgcccatgc gagcaatttg
    26401 ctgccccggt aaaccttcaa gatgctgatc gcgcagtaaa atggtgccgc cggtgggttt
    26461 gtagaaaccg gtcaggcagt taaaaaccgt ggtttttccg gcaccgttag ggccgattaa
    26521 cgagacgatt tcctgcgggt acagttcaag attgacgttg ttcaccgcca gcaggccgcc
    26581 gaagcgcatc atcagaccgt taacagataa taatggctga ctcatgcctg ctctcctttc
    26641 gctgcgccgt ttttcagctt cagttgcggg cgcgtcatgg gcagcaaacc ctgcggacgc
    26701 cagatcatca tcagcaccat caaaccaccg agcattaaca tgctgtattc gttgaaatca
    26761 cgcatcaatt cgcgcgacac caccagcaga acagccgcca gaatcactgc aaattgcgag
    26821 cccataccgc cgagcaccac tatcgccagc acaaaggccg attcagcaaa ggtgaaggat
    26881 tccgggctga caaagccctg acgcgccgca aacagcgttc cggcaaaacc ggcaaatgcg
    26941 gcgctgatgg taaaagcagt cagcttgata cggcgcgggc ttaagcccag cgaacgacag
    27001 gcgatctcgt cttcacgcaa cgcttcccac gcgcggccca gcggcatccg cagcaggcgg
    27061 ttaatgacaa acaggcttaa caccaccagc agcaacgcca ccaggtagag gaagataacg
    27121 cgatcggacg gatcgtattt caggccaaag aaattactga acgtgtccca gccgccttca
    27181 cgagcggtac ggctgaactc gagtccgaag agtgtcggtt tcgggatctg actgattccg
    27241 ttcgggccgc cggtgatttc agtgttattg agcagcaata tgcgcacaat ttcgccgaaa
    27301 ccaagggtaa cgatcgccag atagtcaccg cgcagacgca gcaccggaaa accgagcagg
    27361 aaacccgccg ccgctgccat cagtccggca atcggcaaac aggtccagaa ccccagaccg
    27421 taatagtgat tgagcagcgc aaaagtgtaa gcgccgatgg cgtaaaaacc gccgtacccc
    27481 agcaccagca gaccagaaag accaacaacc acattcagcc cgagaccgag gataatgtag
    27541 atcatggtca gagtggcgat atccaccgtc ccacgtgaca ccataaacgg ccacgccacc
    27601 acaagcacca gcagcgccac gaggaacagc ttttgcttca ccgtggaccc atcaatcgcg
    27661 ggcagaataa acttcggccc ggaaacgctt ttcaaccctt tctggaaagc cggtcgcaaa
    27721 agctggaaga aaaagaccac cgccgtgccg ataaacatcc actgccaacg gatgtctgaa
    27781 gccgtgtcga ccaccagttt ggtgccatcc agctccagtt gtacgcccat aaagacgccc
    27841 gccaggacaa agaacatcgc ggcagagagc agtgccattg caatatgcat cggtttcata
    27901 ctttctctac ctccgggcga cccagaatac cggtcggcat caccagcagc accagaatca
    27961 gcagggcgaa tgagaccaca tctttatatt ccgtactcag ataggcagaa gagagcgcct
    28021 ccgcaatccc caggatcagc ccgccaatca tcgcccctgg aatgctgcca atcccaccga
    28081 gcaccgcagc ggtaaaggct ttcatcccgg ccataaagcc gatatagggg ttaatgacgc
    28141 agtagaactg accgagcagt acgccagcca ctgccgccat cgccgcgcca ataacaaagg
    28201 tcaaagcaat cacccggtcg gtgttgatgc caagcaggct cgccattttc agatcttccg
    28261 cgcaggcacg acaggcgcgc cccatacggg aatagcgaat gaaaatcgtc agcgccagca
    28321 tggcgaggaa ggtgacaatc cagatcaccg cctgcatggt ggtaatagag gcagagaagt
    28381 tttcgctatg gcccaccacc cactgaccgt taaacaggct cggcagcgcc acgtcgcgcg
    28441 aaccttcggt caggctgacg tagttttgca ggaagatgga cataccgatt gcggaaataa
    28501 gcgcaatcag gcgcttagag ttacgcaccg ggcggtaagc tacccgttcg atactccagc
    28561 cgtaggcgct ggcgatgacg attgcgccga cgaatcccgc cgccaccagc agccagccgg
    28621 tatcaatgcc catcatcatc agcgcggcga tgatcataaa tgagacgtag ctgccaatca
    28681 tataaacctc gccgtgggcg aagttgatca tgccgataat gccgtaaacc atggtgtagc
    28741 cgatggctat cagcgcgtag gtactgccca gcgtgacgcc gttaaacatc tgctgcaaga
    28801 aatacaaaaa ctgctcagac ataaggtaac ctttctaaac ccgcccgcat tttacgggcg
    28861 gtgggatgat cacttggctg ccgtggatga accgtcggcg tgccactgga agacaccaaa
    28921 atcaaatccc ttaagatcgc ctttttcatc ccagttcagc ggcccaatca cggtgtttgc
    28981 accgttagct tttaaatctt tcaccagcgc cagcggctca tcgctgccgg tacgctcaag
    29041 ggcagttgcc agagattgca ctgccgcgta agtgatccag acataaggcc cggacggatc
    29101 tttcttgtct gctttcagcg catcaacgat gccctggttt gccggatcct ggtcatagcg
    29161 ttttggcata gtgaccaaca tgccttcggc ggcatcaccg gcaatgttcg acagcgatgc
    29221 gttaccgaca ccttccggcc ccataaactg ggttttcagg ccaacggaac gggcttggcg
    29281 cagcatctgc cccatttccg ggtagtaacc gccgtagtaa acgaagtcga tattttcttt
    29341 tttcaggcgg gcgatcagcg cggagaaatc tttctccccg gcggtaatac cgtcgaagaa
    29401 gacgacgttg gcgttagccg ctttcagccc gtcctgcacc gaacgcgcca gcccttcgcc
    29461 atactgttgt ttgtcgtgaa tgatggcgat gcgctggggc ttcaccgtct caagaatgta
    29521 ttttgccgcc gtaggcccct gggaagagtc cagcccggca gtacgcataa tgtgttgata
    29581 accgcgttgg gtcagctccg ggttggtcgc tcccggcgag atcatcagaa taccttcgtc
    29641 ttcgtagata tccgacgctg gctgggtaga ggaagaacac agatgaccaa taacgtattt
    29701 aatgccgtcg ttaacgattt tgttggcgac cgcaacggct tgtttcgggt cgcaggcgtc
    29761 gtcatattcc acgccaacca gcttgtcgcc cttaattccc cctttgacat taatgtcttt
    29821 aatcgcctga cgcgcgccgt taaattccat atcgccccac tgggcaatcg ggccggacat
    29881 cgcgccgaca acggcgactt taatatcgtc agccatagcg gtgtgtgaaa ttgccagtgc
    29941 aatcatccct gcgatgatag ttttcgcatt ccgtttcata gtcaaaaatc cccattcgtg
    30001 atgttgtgtt gctttgtttt tatgtgttaa caaatcagac tgttcttttt ttatactgca
    30061 ctgtttttgc ctgtctgatt aaggggttag cgcagtattt tgtgatgata gcgattaaaa
    30121 tccctatttt tcagtcgatt aagaacagat aatattctga atttattgat agataaacag
    30181 aaaaaagtgc ctttgtcagc ataaaataac ggcacaaagg gcggaataat tcactatcat
    30241 tcaggggatt atgctggaca tttttcattc tctaatgttt taactttgta attattgctg
    30301 ttaaaaaatt aatcacctgc caaaagaaat aaaaaagaga aagcctccga ttaaattatt
    30361 tcgctacact ggttccactt ttgtgattta cacgggttac ccatgaagct gaccatcatt
    30421 cgattagaaa actttagcga ccaggaccgg attgacctgc aaaagatctg gccggagtat
    30481 tccccttcct ctttacaggt tgacgataac caccgtatct acgccgcgcg ttttaacgag
    30541 cgtctgcttg cggccgtgcg ggtaacctta agcggcaccg agggagcact ggattctcta
    30601 cgcgtgcgag aagtcacccg ccgtcgcggt gtggggcaat atctgctgga agaggttttg
    30661 cgtaacaatc ctggcgtttc atgctggtgg atggcggatg caggcgtgga agatcgcggt
    30721 gtgatgacgg cgtttatgca ggcactggga tttacggcac agcagggcgg ctgggagaag
    30781 cgttaatcgt caagttgatt tcaaaagtga tgaccaacga gaaattgcct gatgcgctac
    30841 gcttatcagg cctataatgt gtgctgcaat ttactgattt ctttggttct tgtaggccgg
    30901 ataaggcgtt tacgccgcat ccggcatgaa gcaacgtatt cgatattagc aatttggcgg
    30961 caacccaaag ttgccgatta acaattactt cgcatcggtc gccgtgccgt tggcgtgcca
    31021 gtcaaatacg ccgaactcaa agccctttag atcgcctttc tcatcccagg tcagtggccc
    31081 catcacggta tccacggagt tcgcttttag gtatttggcg atttcagccg gatcgtcaga
    31141 ctgattcagg cccgcctgca aagattgcag cgcggcgtag gtggtccaga cgaatgcgcc
    31201 gcttgggtcc tgttttttcg ctttgatcgc atcaacaatg ggtttgttcg ccggaacctg
    31261 atcgtagttc ttcggcttgg tcaccagcag ctcttccgct gattcgcccg caatgttaga
    31321 cagcgaaacg ttagccacac cttccggccc cataaactga gttttcagcc ctgccgcgcg
    31381 tgcctgacgc aggatttgcc ccatttccgg gtgataaccg ccgtagtaaa cgaagtcgat
    31441 attctctttt ttcagacgcg ccaccagcgt tgagaaatct ttttccccgg cggtgatacc
    31501 atcaaagaac accacgttag cattgccttt cttcaggccg tcctgcaccg ctcgtgccag
    31561 accttcgccg tattgctgtt tgtcgtgaac gatagcaata cgctgcggtt tcactttctc
    31621 aagaatatat ttcgccgccg tcggcccctg gtcggagtcc aggccggtgg tgcgcaggat
    31681 cagctgatag ccacgggcgg tcagctccgg cgcggttgcc gctggggtga tcattaaaat
    31741 gccttcgtct tcgtagatgt cagacgcagg ctgcgttgat gaagaacaga ggtgaccaat
    31801 cacatattta atgccgtcgt taacgacttt gttcgccacc gcaaccgcct gtttcgggtc
    31861 acaggcatcg tcatatttta cgatttgcag tttgttgcct ttaatgccgc ctttagcgtt
    31921 gatatccgca accgcctgct ctgcgccggt aaactcctgg tcaccgtact gcgcaaccgg
    31981 accggacatt gcacccacga ccgcgacttt aatatcttct gccagagcca tattgctgaa
    32041 tgccagcgcg atacatcctg ccagtaacgc tttacccttt atgttcatcc tgagaatccc
    32101 cattcttctg gttattacgt gtgttgtgat gttgtcgttc agcactttat ttcgttttat
    32161 gcatgactac ccgtgcttta gcagcatact ctgctaaaac atacccgatt tttatgatat
    32221 tggaatagct attttgacag tttattaaca atctgcgtgg ggattggcgt tttgccggag
    32281 ggggaattga ttattgtaga ggcccaaaaa acaaaaaccc cggactctca tccagggttc
    32341 tctgcttaat agcggaaatt acgcttcaat agcggcgcga agttttttca tcgcgttctt
    32401 ttccagttgg cgcacacgtt cagcggaaac accgtaacgg tcagccagtt cctgcaacgt
    32461 ggacttgttg tcttcgtcca gccagcgcgc acggatgatg tcctggctgc gttcgtccag
    32521 accatgcatc gcgtcggtca gacggtttgc cgcctgctct tcccagttat catcttcgat
    32581 gccgtcggca aagttagatg atttatcctg cagatagagc accggtgcca tcggctggct
    32641 gtcggaatcg tcgtcggaag acaggtcaaa ggtcatgtcc tgtgccgcca tacgtgattc
    32701 catctcacgt acgtctttgc tggttacgcc cagttcacgg gccaccattt cgacttcatc
    32761 ctggttaaac cagcccagac gctgcttggt tttacgcagg ttgaagaaca gtttgcgctg
    32821 cgctttggtg gtcgcaactt tgacgatacg ccagttacgc agaacgtatt cgtggatctc
    32881 tgctttgatc cagtggacgg cgaaggagac caggcgcaca cccacttccg ggttaaaacg
    32941 gcgcactgct ttcatcaggc cgatgttacc ttcctgaatc aaatccgcct gtggcaggcc
    33001 atagcccgca taattacgag caatatgaac aacaaaccgc aggtgagaca ggatcagcgt
    33061 tttagctgct tccagatcgc catggtaatg cagcttttca gccagcgccc gctcctcgtc
    33121 agccgacaac atcggccacg cgttagctgc ccggatgtag gaatccaggt tgccaactgg
    33181 ggctaaagct aaactttgca ttttgtcagt cattcaaatc ctctcaatcg atatcttctg
    33241 gcgcttcagt ggtagcaaca actgtgccaa agcttaagag caacgaggtt atcattcact
    33301 gttttatcag accgtgattt tatccacaag ttcaatgcaa gcttgtgaat aaattacgca
    33361 caaaatgtga catagagatg aaataccggg aagagacaac ggggtctctt tccctgctac
    33421 ggaacccatt gcagggaaag agtataacac gcttttatta ttcaggcgta aagtggcgta
    33481 aatgttgtac cgtggcaagc cacgctgcca cccagccaat catcgagcat accagcagca
    33541 atagcaggca ttcatcgaac gataaaccat tgatatcaaa cttcgttccg aaaacctgtg
    33601 ccacttccgc caccgctgac gacaaacgca gtaccagaat ttctgacaaa attaatgaca
    33661 acaatgcgcc agaaaatccc agcagcgcac cgccgtacag gaacgggcga aggatgaaac
    33721 catccgtcgc accaatcagt ttctgcacgt tgatggagtc acggcgagca aagatgctca
    33781 gacgtacgct gttgccgatg acgaggaaca ctgccgccac catcaacacg ccgatcatcg
    33841 ccgaaacgcg cccaaccagc ccggtcaacg ccgccagacg ggcaaaccag ctgtcatcca
    33901 tccgcacttc gtcaataccg ttaatctgcg tgatacgatc acgcagcgta ttgagtgatt
    33961 ccgtcccctg aaaatcgagt ttcgggatca ccaccgccac tgccggaagc gggttttctt
    34021 ccagcatatc cagcgcacca ccaaaaccag accagttacg gaactcgccc agtgcgtctt
    34081 cacgagaaag atagttcact ttctccacgc cttgctcggc ctgcaactgt gccaccacgc
    34141 ccgcagcggc gtcatcgtcc agcgtttttt gcaagtaaac agtgatttgc ggtgacggat
    34201 aatactgcgt cgccgcctga ttaacgtttt tgtacaccat ataacagacg ctgggcagcg
    34261 tcagggagat ggcgataacc atcaccgtta aaaacgtggc gaacggtttg cttttcagat
    34321 cctgcaatgc gccgtggaag gcatagcgca cctgttcgtt gaaaacgttg gttttgcgat
    34381 ttaccggttt tggcgaggat ttcgcgcgtt tcggtgcgtt acgaccgccg tcgcctgagc
    34441 cgccgaccga tttacggaag cgctccagac gcccaccaaa ctgccgaata tgattgattg
    34501 catcgcgctt attcatggcc cacgcctcca tgcaagtgac catcgctcag ggtgagcatg
    34561 cgataggaac gccgtgaaat caggttaatg tcgtgcgttg ccatcaatac ggttacccca
    34621 acgcggttaa actcttcaaa cagacgtaga atcccttccg acaacgcgtc gtccaggtta
    34681 ccggtcggtt cgtccgccag cagtaccgcg ggcttattca ccaccgcgcg ggcaatgcca
    34741 acacgctgtt gttcaccgcc ggaaagctga atagggaagt tcttcgcttt gtccagtagc
    34801 ccgactttat ccagtgccgc cgacacccgg cgacgaatat cgtcaccgct ggcaccagcg
    34861 ataatcagcg ggatcgccac gttatcgtag acagtacggt ccatcagtag atggtgatcc
    34921 tggaaaatca tgccaatctg gcggcgcaga aacggaactt cacggttttt cagacgcgtg
    34981 atgtcatggc cgctaaacca gattttcccg gcgctgggcc gctcaatccc acagatcagc
    35041 ttcaggaggg tacttttccc tgcgccggaa tgaccggtca gaaacgccat ctcacccggc
    35101 tgcatatgga acgtaacgcc ctgcagcgcc tgtctcccac cgagataagc cttgctgaca
    35161 tgttcaaagc gaatcattgt taatcctctc gggcaaaaag tgcctctata aagtcgtccg
    35221 ccttaaacgg acgcaaatcc tcaatacgtt cgccgacacc aatgtagcgg atagggatac
    35281 caaactggtc agccaccgag aaaattaccc cgcctttcgc cgtgccgtcc agtttcgtta
    35341 gcgtgatgcc ggttaagcca acagcttcgt ggaacagttt ggcctggctt accgcgttct
    35401 gcccggtgct ggcatcaata gtcagcataa cttcatgcgg cgcttcaacg tcgagtttct
    35461 tcatcacgcg gacgattttc ttcaactctt ccatcagatg cgatttgttc tgcagacgtc
    35521 cggcggtatc ggcaatcagg acgtcgatat tacgcgcttt agctgcctga atggcgtcga
    35581 agataacaga ggcggaatcc gcaccggtat gctgggcaat caccggaata ttgttgcgct
    35641 gaccccagac ctgaagctgt tcaaccgcag ctgcacggaa agtatcgccc gccgccagca
    35701 tcaccgattt accctgctgc tcaaactgac gcgccagctt accaatcgtc gtggttttac
    35761 ccacaccgtt gacgcccacc atcaggatca cgaacggcgt tttatcttca acattcagcg
    35821 gctcatcgac tttcgccaga atctcgccca tctcttcttt cagcaggcca tagagcgcct
    35881 cggcatcacg aagctgcttg cgggatgcgc cttccgtcag attggtgata attttacgtg
    35941 tggtttccac gcccacatcg gcgatcaaca gctgttcttc cagctcttca aatagatcat
    36001 cgtcgatttt tttaccgcgg aacaggctga taaatccgga accgagattt tctttggttt
    36061 ttaacaggct gcgtttcagg cgcgcgaaga agccttcttt ggtcggtttt tcttgctcct
    36121 gggcaatttc tgcaaccggc tgctcttctt ctgccggagg aactaccatc accgcctctt
    36181 ctgccgcttc ggcagccagc gcctgtgctt ccagctcttc gtcggtgatt tcttctttag
    36241 ccgcttcttc ttctgccgct tcgacaatct ctacggtttc cgcttcagcc tgccactctt
    36301 ctggcgaaac ctcttcggcg ttgacgtctt ccggcaacgg cagctcttca cgttcgatag
    36361 ccactggctc cggcgtttct tctacgaccg gttccggctg tgcaacaact tccgcttcag
    36421 gctgcgcttt ttcactttca acaacctgtt cagtgacttc cacaacgtcg gcagcaaaag
    36481 tttccgcctc ggcttcagta tgcgcctgcg gctgctcttc aacggcttgt tcagaagcct
    36541 tcgcaggctc ttgcgcctga acgatttctt ctacaaccgg ttgttcattc tgaacttctg
    36601 tctctttttc cggggtctgc tctttttgac caaagcccag ccaggaaaaa aagccacgtc
    36661 ttttttcttt cgccatttgc gactacactc ctcgctgttg cttcatggca cagcgttaac
    36721 gctatgtaca tagcagctaa aaaaatgatg aaatagtcta tcacttaact taattcacat
    36781 caccgcctgc aagtatgtgt tatctggcgg attgagcaat ttatcatgaa aaaaccgaat
    36841 cattccggca gcggccaaat ccgcattatt ggcgggcagt ggcgaggccg taaactcccg
    36901 gttcctgata gcccaggtct gcgccccacc accgaccgcg tacgcgaaac gttgtttaac
    36961 tggctggctc cggtcattgt tgacgcccaa tgtctggatt gcttcgccgg gagcggcgcg
    37021 ctggggctgg aagcgttatc gcgctacgct gcgggggcaa cgttgattga gatggatcgc
    37081 gtggtttctc agcagttaat taagaatctg gcgacactaa aagcaggcaa tgcacgcgtg
    37141 gtgaacagca acgcaatgtc attcctggcg caaaaaggca caccgcataa tatcgtgttt
    37201 gtcgatccac cgttccgccg tggcttgtta gaagagacga taaatttact ggaagataac
    37261 ggctggctgg ctgacgaagc cctgatttat gtcgaaagcg aagtcgaaaa cggtctgccc
    37321 actgttccgg caaactggtc attacatcgg gaaaaagtgg cgggtcaggt ggcttatcgg
    37381 ctgtatcaac acgaagcaca aggagaaagt gatgctgatt aatattggtc gtttgttaat
    37441 gctctgcgtt tggggatttt taatcctcaa tctggtgcat cccttcccgc gcccgctaaa
    37501 tattttcgtt aacgtggcgc tgatttttac cgtgctgatg catggtatgc agctggcgct
    37561 attgaaatcc actttaccga aagatggccc gcagatgacc accgccgaaa aggtgcggat
    37621 tttccttttc ggcgtgtttg aactgctggt ctggcagaag aaatttaaag ttaaaaaata
    37681 accttattgt tcgccaacaa agctaacaaa gcgcgttcct ttatagctca gcgtaccttt
    37741 atcgcccact gtcagggcgt ggtactgctg ggcgtcgagg cgaaacgtct gctccattcc
    37801 tccgctttgc ggtttgaagc ttgcctcata gcgcatactg gtgcctgccg gagtcacttc
    37861 ctgctggcgc gaacggcgat cgttaatcgg tttttcccgc ttgttgctca ccaccaccag
    37921 cttttgctgg agcggagcca tatcattatc agctttttcc cgtcgctgtt gcataaaacg
    37981 aaacgatgcg gcgacgacaa ttaagccaat gataacaata aagaaaagag gtggtttact
    38041 catctttatc cctcatcgga aaatgcggaa ttaagcatac cctgccagtt atggtgttgt
    38101 catccgtcca ccctcgccac taaactggaa gcaagaccgt aggcattctg cttacgaaaa
    38161 aataacgaat tcaaggaact aagatgcttt ggtcgtttat cgctgtctgt ctttccgcat
    38221 ggctatctgt ggatgcatcg tatcgtgggc caacctggca acgctgggtg tttaaaccgt
    38281 taacccttct tctcctgctg ttactggcct ggcaagcacc gatgttcgac gccattagct
    38341 atctggtgct ggcgggactg tgcgcctcac tgctgggcga tgcgctaacc ctgttgccac
    38401 gtcaacgtct gatgtacgcc atcggcgcgt ttttcctctc gcacctgctg tacaccatct
    38461 atttcgccag tcagatgacg ctctctttct tctggcctct gccactggtg ttgctggttc
    38521 tgggtgcgct gctactggcg attatctgga cgcggctgga agagtaccgt tggcctatct
    38581 gcacgtttat cggcatgacg ctggtgatgg tgtggctggc aggtgaactg tggttcttcc
    38641 gtccgaccgc tccggcgctc tctgcgtttg ttggcgcttc gttgctgttt atcagtaact
    38701 ttgtctggct ggggagccac tatcgccgac gcttccgtgc ggataacgcg attgctgcgg
    38761 cctgctactt tgccggtcac ttcctcatcg tccgctcact gtatctctga taaaacttga
    38821 ctctggagtc gactccagag tgtatccttc ggttaatgag aaaaaactta accggaggat
    38881 gccatgtcga cacctgacaa tcacggcaag aaagcccctc aatttgctgc gttcaaaccg
    38941 ctaaccacgg tacagaacgc caacgactgt tgctgcgacg gcgcatgctc cagctcgcca
    39001 acgctctctg aaaacgtctc cggcacccgc tatagctgga aagtcagcgg catggactgc
    39061 gccgcctgtg cgcgcaaggt agaaaatgcc gtgcgccagc ttgcaggcgt gaatcaggtg
    39121 caggtgttgt tcgccaccga aaaactggtg gttgatgccg acaatgacat ccgtgcccag
    39181 gttgaatctg cggtgcaaaa agcgggctat tccctgcgcg atgagcaggc cgccgacgag
    39241 ccacaagcat cgcgcctgaa agagaatctg ccgctgatta cgcttatcgt gatgatggca
    39301 atcagctggg gtctggagca gttcaatcat ccgttcgggc aactggcgtt tatcgcgacc
    39361 acgctagttg ggctgtaccc gattgctcgt caggcattac ggctgatcaa atccggcagc
    39421 tacttcgcca ttgaaacctt aatgagcgta gccgctattg gtgcactgtt tattggcgca
    39481 acggctgaag ctgcgatggt gttgctgctg tttttgattg gtgaacgact ggaaggctgg
    39541 gccgccagcc gcgcgcgtca gggcgttagc gcgttaatgg cgctgaaacc agaaaccgcc
    39601 acgcgcctgc gtaacggtga gcgggaagag gtggcgatta acagcctgcg gcctggcgat
    39661 gtgattgaag tcgccgcagg tgggcgtttg cctgccgacg gtaaactgct ctcaccgttt
    39721 gccagttttg atgaaagcgc cctaaccgga gaatccattc cggtggagcg cgcgacgggc
    39781 gataaagttc ctgcaggagc caccagcgta gaccgtctgg taacgctgga agtgctgtca
    39841 gaaccgggtg ccagcgccat tgaccggatt ctgaaactga tcgaagaagc cgaagagcgc
    39901 cgcgccccca ttgagcggtt tatcgaccgt ttcagccgta tttatacgcc agcgattatg
    39961 gccgtcgccc tgctggtaac gctagtgccg ccgctgctgt ttgccgccag ctggcaggag
    40021 tggatttata aaggtctgac gctgctgctg attggttgcc cgtgtgcgtt agttatctca
    40081 acgcctgcgg cgataacctc cgggctggca gcagcagcgc gtcgtggggc gttaattaaa
    40141 ggcggtgcgg cgctggaaca actaggtcgt gttacccaag tggcgtttga taaaaccggt
    40201 acgctgaccg tcggtaaacc gcgcgttacc gcgattcatc cggcaacggg tattagtgaa
    40261 tctgaactgc tgacactggc ggcggcggtc gagcaaggcg cgacgcatcc actggcgcaa
    40321 gccatcgtac gcgaagcaca ggttgctgaa ctcgccattc ccaccgccga atcacagcgg
    40381 gcactggtcg ggtctggcat tgaagcgcag gttaatggtg agcgcgtgtt gatatgcgct
    40441 gccgggaaac atcccgctga tgcatttgct ggtttgatta atgaactgga aagcgccggg
    40501 caaacggttg tgctggtagt acgtaatgat gacgtgctgg gtatcattgc attgcaggat
    40561 accctgcgcg ccgacgctac aactgccatc agtgaactga acgcgctggg ggttaaagga
    40621 gtgatcctca ccggcgataa tccacgcgca gcggcggcaa ttgccgggga gctggggctg
    40681 gagtttaaag cgggcctatt gccggaagat aaagtcaaag cggtgaccaa gctgaatcaa
    40741 catgcgcccc tggcgatggt cggtgatggt attaacgacg cgccagcgat gaaagctgcc
    40801 gccatcggga ttgctatggg tagcggcaca gacgtggcgc tggaaaccgc cgacgcagca
    40861 ttaacccata accacctgcg cggcctggtg caaatgattg aactggcacg cgccactcac
    40921 gccaatatcc gccagaacat caccattgcg ctgggactga aagggatctt cctcgtcacc
    40981 acgcttttag ggatgaccgg gttgtggctg gcagtgctgg cagatacggg tgcgacggtg
    41041 ctggtgacgg cgaatgcgtt aaggttgttg cgcaggagat aaggcaaacc gatcgcaaca
    41101 ttgagcgcga tcggtcccct cgcccctctg gggagagggt tagggggtga ggaaaaggcg
    41161 gcatcgaagc caatcagccc ctatcaaccg cctttacgaa tcaaataacg ataaggcagt
    41221 ccatccgtct ctttcgcaac cagttcgtgt tccataaagg tacaaaaccc aggaatatcg
    41281 cgggtagtgg ccggatcgtc ggcgataatc agcaacgttt cgccaggctg catattgcgc
    41341 acggttttgc gcaccatcat caccggttcc gggcagcgca ggccaagcgc gtcgagtgtg
    41401 tggtcagggc tggaaaagag atcggtcatt ttcttctcat cacttaaaaa aacggcgcta
    41461 gtttacgccc tgtgagtccg taagcaaacc aggttaacga ttgcgtgaaa attaaccatt
    41521 gcattgtcaa cgtaaagcag tatcatgcgg cggctcgaaa aaagggtaag cacgttatta
    41581 tgttaaggta acagacgtgt cgtacgtatt gggttccctc accccaatgg ttaatcaaaa
    41641 aggtacaata tgaacgtttt ctcgcaaact caacgctata aggcgttgtt ctggttatcg
    41701 ttatttcatc ttctggtgat cacctccagt aactatctgg ttcagcttcc cgtctccatt
    41761 tttggtttcc ataccacctg gggcgcgttt agctttccgt ttatttttct tgctaccgac
    41821 ctgaccgtgc gtatttttgg cgcaccgctg gcccgacgca ttatcttcgc ggtaatgatc
    41881 cctgcgttat taatctccta cgtcatctcg tcgctattct atatgggttc ctggcaggga
    41941 ttcggcgcac tcgcccactt caacctgttt gtcgcccgta tcgccaccgc cagtttcatg
    42001 gcctacgcgc tggggcaaat cctcgacgtg cacgttttta accgcctgcg tcagagtcgc
    42061 cgctggtggc tggcaccgac agcgtccaca ctgttcggta acgtcagcga cacgctggcc
    42121 tttttcttca ttgccttctg gcgtagcccg gatgccttta tggctgaaca ctggatggaa
    42181 atcgcgctgg tcgattactg tttcaaagtg ttaatcagta tcgttttctt cctgccaatg
    42241 tatggcgtat tactcaatat gctgttgaaa agactggcag ataaatccga aatcaacgct
    42301 ttgcaggcga gttaaaggtt cgttatcaga gttgtgataa gatggatgaa tgagccgtta
    42361 tggccgttta tcgaaaggaa gaagtcaatg cgcaatctgg ttaaatatgt cggaattggc
    42421 ctgctggtta tggggcttgc ggcctgtgat gataaagaca ctaacgctac ggcgcagggt
    42481 tcggttgcgg aaagtaacgc taccgggaat cccgtcaacc tgcttgatgg caagttaagt
    42541 ttctcgctgc cagcggatat gaccgaccag agcggtaagc tgggaacgca ggccaataac
    42601 atgcatgtct ggtccgacgc caccgggcag aaagcagtca tcgtcatcat gggcgatgat
    42661 ccgaaagaag atctggcggt gttggcgaag cgtctggaag atcagcaacg tagccgcgat
    42721 ccgcagctgc aagtggtaac caataaagcc attgagctga aaggtcacaa aatgcagcag
    42781 ttagacagta ttatctccgc gaaaggccag acggcgtact cttccgttat tctgggtaac
    42841 gtgggtaatc aactgctgac catgcaaatt acgctgccag ctgacgatca gcaaaaagcg
    42901 caaaccaccg cagaaaacat cattaatacg ctggttattc agtaagtttt aagatgatga
    42961 ggcggcctca gggacgtgtt ccggaggccg tttttttaat cgccacgtca gtaataacgc
    43021 gattgcgacc agtcccgccg ccgccagata aatcaccggt acgcccgccc agctcatcac
    43081 cagcccagcc agtggtccag tcacgccaag cgataaatcc ataaatacgg tgtaagttgc
    43141 cagcgccgcc ccctgatttt gctgcggaac cgcttttacc gccactacac ccaatgccgg
    43201 gaacaccagc gaaaacccgg cccccgccag taagacgccg attttcgcca tccacggcat
    43261 agtcgccacg ccaaccagta gcaggccgat tatctcaacg ctaaagcaaa tcatcgctac
    43321 gtttaagcca ccgatacggt taatgccgtt agggaataac aaacgcgtac cgacaaacgc
    43381 acagctaaac agcgtcagcg cgaaagccgc accgtcccaa cctttagcgt cataaaacag
    43441 cgtgataaag gtggcgatga cgccaaatcc ggcggaagcc agtgccagcg ccataccgta
    43501 cagccagacg cgcccaagca ccgcgcgaaa cggcagcggt ttgcctttac tggcttttac
    43561 cgtcggacgc gggatcgcca acaaaatggc caccagcgcc acgcccataa tgattaacgc
    43621 taacgcctgc aagccgcccc agtgataaaa cacgacgcct aacggcgcac ccatcgccat
    43681 cgccccgtaa gtgacaatgc cgttccacga aatcacccgc ccgatatgca gcgagccaac
    43741 cactccaacg ccccataggg tcgatcccgt tccggcaaaa ctttgcccaa tcccaaggat
    43801 gacgcgcccc aggcaaagta ataacaggct gatgacaggc agactggcgg ttaatcctgc
    43861 cgtcagatac cccagaccgc tcaaaaagca gccgcataaa ccgaagacga caatcttttt
    43921 gggtcccagc aaatcggcgt aacgtccggc atgagggcgg ctcagcaagg tggcgaaata
    43981 ttgcaggctg ataaccaatc ctgcccagaa ggcgctaaag cccatcacat catggacata
    44041 gcccggtaat acagcgagcg gcaacccgat ggtgaggtag ctggcgaagt taaacatgac
    44101 aatggagaca atgcgcaaat tcaggcgcaa tccgtttagc gcgggttcgg ctacgggttc
    44161 gggcatgagg atcaccacat ttttacaaca gtgtttcatt tttaccatgt gctgacgtga
    44221 aaatcagcag taagaatcag aatattgctg gcgtgactcc cgcgctacac ttaacacaaa
    44281 aagtcacaag gaagccccaa tggaaacccc tcaacccgat aaaacgggca tgcacattct
    44341 gctcaagttg gcctcgctgg tagtgatcct cgcgggcatt cacgcagcgg cagatatcat
    44401 tgtgcagctg ttactggcgc tgttttttgc catcgtcctc aacccgctcg ttacctggtt
    44461 tattcgtcgg ggagtacaac gccccgttgc cattacgatt gtagtggtgg tgatgctgat
    44521 cgcactaacc gcgctggtcg gcgtactggc ggcatcgttt aacgaattta tctctatgct
    44581 gccgaagttt aataaggagc tgacgcgcaa actttttaaa ttgcaggaga tgttgccttt
    44641 tcttaatttg catatgtcgc cggagcgaat gctgcagcgg atggactcgg aaaaagtggt
    44701 taccttcacc acagcgctaa tgaccgggct ttccggggca atggcgagcg tgcttttgct
    44761 ggtgatgacc gtagttttta tgctgtttga agtgcgccac gtcccttaca aaatgcgttt
    44821 tgcgctgaat aacccacaga ttcacatcgc aggattacac cgcgcactta aaggcgtttc
    44881 gcattatctg gcgttaaaga cgctactcag tttatggaca ggtgtcatcg tctggctggg
    44941 gctggcgcta atgggcgtac agtttgcgct gatgtgggca gtactggcgt ttttgctcaa
    45001 ctacgtgccc aatatcggcg cggtaatttc cgccgtaccg ccaatgattc aggtgctgct
    45061 gtttaatggt gtttacgaat gtattctggt cggcgcattg tttttagtgg tccatatggt
    45121 catcggcaat attttagaac cacggatgat gggccatcgc ctggggatgt ccaccatggt
    45181 ggtatttctt tcattgttaa tctggggatg gctgctcggc ccggtaggga tgctactttc
    45241 ggtgccatta accagcgtgt gtaaaatctg gatggaaacg acgaaaggtg gcagcaaact
    45301 ggcgatttta ctggggccgg gcagaccgaa aagtcggtta ccgggatgag gcgacaagtg
    45361 atacgatacg cactttcatt ttccattaaa cgttggccct gatatgtatc ggatagttct
    45421 ggggaaagtt tcgaccttaa gcgcagctcc actgccaccg ggtttacgcg agcaagcacc
    45481 gcaaggtcca cgacgcgaac gctggctggc ggggcgtgca ttgctttcgc acacgctttc
    45541 cccgctaccg gagatcatct atggcgaaca aggcaaacct gcattcgcgc cggaaacgcc
    45601 gctatggttc aacttaagcc atagcggtga cgatatcgct ctgctgttga gcgatgaagg
    45661 cgaagtcggc tgcgatatcg aagtgattcg cccgcgcgcc aactggcgct ggctggcgaa
    45721 cgccgtgttc agcctcgggg aacacgctga aatggacgcc gtgcatcctg atcagcaact
    45781 ggaaatgttc tggcgcatct ggacgcgcaa agaagccatc gttaaacagc gtggcggcag
    45841 cgcctggcaa atcgtcagcg tagatagcac ctatcactcc tcgctgtcag tcagccattg
    45901 tcagctagaa aatttaagcc ttgcgatctg cacccccacc ccctttacgc tcaccgccga
    45961 cagtgtgcaa tggatcgatt cagttaactg atccgcccac ccgactgccc atctattgat
    46021 ccagaacagg taatcagtat gacgaatact taaaatcgtc atacttattt ccgccatcta
    46081 ttttaatcca ttggggttac catgctctcc acactccgcc gcactctatt tgcgctgctg
    46141 gcttgtgcgt cttttatcgt ccatgccgct gcaccagatg aaatcaccac cgcctggccg
    46201 gtgaatgtcg ggccactaaa cccgcacctt tacacgccta accagatgtt cgcccagagc
    46261 atggtttatg aaccattggt gaaatatcag gcagacggtt cggtgatccc gtggctggca
    46321 aaaagctgga ctcattcaga agatggtaaa acctggacct tcaccctgcg tgatgacgtg
    46381 aaattctcca acggtgaacc gttcgatgcc gaggcggcgg cagaaaactt ccgcgcagtg
    46441 ctcgataacc gtcaacgtca cgcctggctg gagctggcaa accagattgt tgatgttaaa
    46501 gcactcaata aaacagagct gcaaattacc ctgaaaagcg cctactatcc tttcctgcaa
    46561 gaactggccc tgccccgtcc tttccgtttt atcgctccct cgcagtttaa aaaccatgaa
    46621 accatgaacg gaattaaagc gccgattggc accggaccgt ggattttgca ggaatcgaaa
    46681 ctgaatcagt acgatgtctt cgtccgtaac gaaaactact ggggcgaaaa gccagcgatt
    46741 aaaaagatca cctttaacgt catcccggac ccgactaccc gcgcggtggc gtttgaaact
    46801 ggcgatatcg acctgctgta cggaaacgaa gggttattac cgctcgatac cttcgcccgc
    46861 tttagccaga atccggctta ccacacccaa ctgtcacagc cgatcgaaac cgtgatgctg
    46921 gcgctcaata ccgccaaagc ccccaccaac gagctggcag tacgtgaagc tcttaattac
    46981 gcggtaaaca aaaaatcgct gattgataac gcgttatatg gcacccagca ggtcgccgac
    47041 accctgtttg ccccttctgt gccttacgct aatctcggcc tgaaaccgcg ccagtacgat
    47101 ccgcagaaag cgaaagagtt gctggaaaaa gccggttgga cgctgcctgc gggcaaagac
    47161 atccgcgaga aaaatggtca gccgctgcgc attgaactgt cgttcatcgg caccgatgcg
    47221 ttaagcaaat cgatggcgga aatcattcag gctgatatgc gccagattgg cgcagatgtc
    47281 tcgctgattg gcgaagaaga gagcagtatc tatgctcgtc agcgcgacgg tcgttttggc
    47341 atgattttcc accgcacctg gggcgcgcca tacgatccac acgccttcct gagttcaatg
    47401 cgcgtaccgt cgcacgctga cttccaggca cagcaaggat tagccgacaa gccgctgatt
    47461 gataaagaga tcggcgaagt gctggcgacc catgacgaaa cgcaacgtca ggcgctgtat
    47521 cgcgacattc tgacccgtct gcatgacgag gcggtttatc tgcctatcag ctacatctcg
    47581 atgatggtgg tatcaaaacc ggagctgggt aatatcccct acgcgccgat cgccaccgaa
    47641 attccgttcg aacagattaa accggtgaaa ccttaatgtt gcgttacgta ttacgccgct
    47701 ttctgctgct gatcccgatg gtgctcgccg cctcggtgat catttttctg atgctgcgcc
    47761 tcggtaccgg cgacccggcg ctcgattatc tgcgtttatc taacctgccg ccgacgccgg
    47821 agatgctggc ctctacccgc accatgctcg ggctggatca gccgctgtac gtccagtacg
    47881 gtacctggtt gtggaaggcg ctgcatcttg actttggtat ctcattcgcc agccaacgcc
    47941 cggtactgga cgatatgctg aacttcctgc ccgccacgct ggaacttgca ggtgcggcgc
    48001 tggtattaat tctgctcact tccgtaccgc tcggtatctg ggcggcgcgc caccgcgatc
    48061 gtttgccgga tttcgccgtg cgctttatcg cgtttcttgg cgtgtcgatg cccaacttct
    48121 ggctggcatt tttgctagtg atggcgtttt cggtgtatct gcaatggcta cccgcgatgg
    48181 gctacggcag ctggcagcac atcattttac ctgcggtttc catcgccttt atgtcgctgg
    48241 cgattaacgc gcgactgctg cgcgccagta tgctggacgt cgccggtcag cgtcacgtca
    48301 cctgggcgcg gctacgcggc ctgaatgaca aacagaccga acgccgccat attctgcgca
    48361 atgcctcgct gccgatgatc accgccgtgg ggatgcacat tggcgaattg attggcggga
    48421 cgatgattat cgaaaacatc tttgcctggc ctggcgtcgg gcgctatgcg gtgtcggcga
    48481 tttttaaccg tgactatccg gtgattcagt gctttacgct gatgatggtg gtggtttttg
    48541 tggtctgtaa tttgattgtc gatttgctca acgccgcgct ggacccgcgc attcgtcgtc
    48601 atgaaggagc gcacgcgtga actttttcct ctcttcccgc tggtcggtac gcctggcgct
    48661 gatcattatc gccctgctgg cgctgattgc gctcaccagc cagtggtggc agccgtacga
    48721 tccacaggca attgatttgc cgtcgtgcct gctttcgccg gatgcgcagc actggctggg
    48781 caccgatcac ttaggtcgcg atattttctc gcggctgatg gcagcgaccc gcgtgtcgct
    48841 cggttcggta atggcctgcc tgctgctggt gctgacatta gggctggtta ttggcggcag
    48901 cgccgggttg attggcgggc gcgttgatca ggccaccatg cgcgtcgccg atatgtttat
    48961 gaccttcccg acctcgattc tgtcgttctt tatggttggc gtgctcggca ccgggctgac
    49021 caacgtaatt atcgccatcg ccctgtcgca ctgggcgtgg tatgcacgca tggtgcgcag
    49081 cttggtgatt tcactacgcc aacgcgagtt tgtgctggcg tcacggcttt ccggtgcggg
    49141 ccatgtgcgg gtgtttgtcg atcatctggc aggcgcggtg atcccttcgc tgctggtgct
    49201 ggcaacgctg gatatcggcc atatgatgct gcacgtcgcg gggatgtctt tccttggcct
    49261 cggtgtgacc gcgccgaccg ccgaatgggg cgtgatgatt aacgacgcgc gccagtatat
    49321 ctggacccag ccgctgcaaa tgttctggcc ggggctggcg ctgtttatca gcgtgatggc
    49381 ctttaacctg gtgggtgacg cactgcgcga tcatctggat cctcatctgg tgacggagca
    49441 cgcacactaa tgccgcaaca gattgaacta cgtaatatcg cgctacaggc cgcgcagccg
    49501 ctggtacacg gtgtatcgtt aaccctgcaa cgcgggcgcg tgctggcgtt agtcggcggt
    49561 agcggcagcg ggaaatcatt aacctgcgcc gcgacgctgg gcattttgcc cgctggcgtt
    49621 cgccagacgg cgggggaaat tttagccgat ggcaaaccgg tttcgcctta cgccctgcgc
    49681 ggcatcaaaa ttgccaccat catgcagaac ccgcgcagcg cctttaatcc actgcacacc
    49741 atgcacaccc acgcgcggga aacctgcctg gcgttaggga aacccgccga tgacgctacg
    49801 cttaccgctg ccatagaagc ggtggggctg gaaaacgccg cgcgcgtgct gaagctgtac
    49861 ccgttcgaga tgagcggcgg catgttgcag cgcatgatga ttgcgatggc ggtgctgtgt
    49921 gaatcaccgt ttatcatcgc cgatgaaccg accaccgacc tcgacgtggt agcacaggcg
    49981 cgcatcctcg atctgctgga aagcattatg caaaaacaag cgccgggaat gctgctggtc
    50041 acccatgata tgggcgttgt ggcgcgtctg gcggatgacg tggcggtgat gtcacaaggt
    50101 aaaattgtcg aacagggcga tgtagaaacg ctgtttaacg cccccaaaca tacggtgacg
    50161 cgcagcctgg tttccgctca tctcgccctc tacggtatgg agctggcatc atgaccttac
    50221 ttaacgtctg cggcctttcc catcactatg cccacggtgg atttagcgga aaacatcaac
    50281 atcaggcggt gctgaataac gtttccctga ccctgaaaag cggcgaaacc gtcgccctgc
    50341 tgggacgcag cggctgcgga aaaagcaccc tcgcgcggct gctggtaggc ttagagtctc
    50401 cctcacaagg gaatattagc tggcacggcg aatcgctggc gaaacttaat cgcgcccaac
    50461 gtaaagcctt ccgccgtgat attcagatgg tgtttcagga ctccatcagt gccgtgaatc
    50521 cgcgcaaaac cgtgcgcgag atcctgcgtg aaccgatgcg ccacctgcta tcactgaaaa
    50581 aatccgaaca actggcgcgc gccagcgaaa tgctgaaggc ggtcgatctc gatgacagcg
    50641 ttctcgacaa acgcccaccg cagttaagcg gcggccagct ccagctcgtc tgcctggcac
    50701 gcgcgctggc ggtcgaaccg aaattgctga ttctggatga agccgtttct aaccttgatc
    50761 tcgtgttaca ggcgggcgtc attcgcctgc tgaaaaagct acaacaacag tttggcactg
    50821 cctgcctgtt catcactcat gacttacgcc tggtggaacg cttttgccag cgagtgatgg
    50881 tgatggacaa cggacaaatc gccgaaaccc aggcggtggg agataaatta accttttcct
    50941 ctgacgccgg acgtgtgcta caaaacgcgg tattacccgc attccccgtg cgccgtcgca
    51001 cctcagaaaa ggtttaacgc caatgcaacg agtcaccatc acgcttgatg acgatttact
    51061 ggagacgctg gacagcctga gccagcgtcg tggttataac aaccgttccg aagctatccg
    51121 cgatattctg cgtagtgccc tggcgcagga agctacccag cagcacggca cacaaggttt
    51181 cgcggtgctg tcgtatgtgt atgaacacga aaaacgcgac ttagccagcc gtattgtttc
    51241 cacccagcat catcaccacg acctctccgt cgccacgcta catgtgcaca tcaaccacga
    51301 cgactgtctg gaaatcgccg tgttgaaagg tgacatgggt gacgtgcagc attttgccga
    51361 tgacgttatc gcccagcgcg gcgtgcggca cgggcatttg cagtgcctgc cgaaggaaga
    51421 ttgagttatt tctaaaacaa ggaaagaaaa gatattaact gaacttaatt tataaaatta
    51481 agttcagttg catagataga tcctgtaaag gcagatattc tgcgtaatgt acgtcgctaa
    51541 aatttataaa tgattcaaac gctcattcag tgtttcaata atataggtat taacagagac
    51601 ttgttgctgc gcagccgcat tattaaggcg ttcactcagc gattctggat agcgtaacgt
    51661 aaaggtctta atcttttctg tacgcgcata cggctcaata cccgcagctt tacaatcctc
    51721 aagatactct cgcagggaaa gctctccttc tttttgcagc ccctgaatac tgtcagagac
    51781 aaaatcacaa taacctgaca agcccaggaa tttgccacga aatgcattca gttctggcac
    51841 gtaggtaata acagctggtt gcccggcaat ctccatagag tttggcgtct tcagtttgat
    51901 catggtttta cccctatact tagtaaccag tcacacacgc tttctacagc gcctttatcg
    51961 gtatctggcg acggatgagg ccgatggaaa cacgcaacgc tcatatttag tatgaacttg
    52021 caacgcgaac ctcttccttt cttaatttct ccgcctaatg ctttaaccag tgactctata
    52081 tcagaccact tgatcccttg aggaacagga gttttaaata tttgatccaa tgtatttttt
    52141 tgtttcttcc gtaaagcctg taccttggtt ctcatggcat gtccttatga cttcacttta
    52201 tgacatcaca gtatgaagtc aagtttccct tccttacgcc attgtcccaa tcgtcttcct
    52261 gaatcgcagc agtgcgatgg tgaaaaacgc gccgccaatt gccatcagcg tcaggaactg
    52321 cggccagacg atttcgaatc cggcaccccg gtagaggatg gcctgcgcga ggctaacaaa
    52381 gtgtgtcgtc ggcatggtca gcataatgtc ctgcaccatc tgcggcatac tttcgcgcgg
    52441 cgtggatcca ccggaaagca tttgcagcgg cagcagcacc agaatcacca gcagccccag
    52501 ttgcggcatt gaacgcgcta tcgtccccat aaaaatgccg attgacgtgg tggcaaacag
    52561 actgagcgcc acgcccagca taaacagcgg gatcgagcct tcaatcggta cgcccagtac
    52621 acctttcacc atcagcacca gcgataatcc cgataccacc agcaccacca gccccatcga
    52681 ccagatcttc gccatcatga tctcaaacgg cgttatcggc atcaccagta aatgttccac
    52741 cgtgccgtgt tcacgctcgc ggatcagcgc cgatccggtc aatacaatcg ccagcatggt
    52801 aatgttgttg atgatcgcca tcaccccgcc aaaccacgcg ggatcaaggt tcgggttaaa
    52861 gcgcatccgg gtttccagcg ataccaacgg ttcgctgtta tcacggtagc gcgcgacaaa
    52921 gctgttcact tcaccgttga taatattctg gatataccca ttgccggtaa atgcctggct
    52981 catgcgcgtg gcatcgacgt tcacctgaat atccggctgg cgtccggcga ggacatcacg
    53041 ctgaaaatta ggcggaatat ttatcgcgaa ggtatagcgt ccggcgtcca gtccggcatc
    53101 catctcatcg gcggtgatca tctccggtgg caaaaaccac ggacgataga agctgttaac
    53161 gatccggttc gataactgcg attgatccat atcggcaatg gcgatcggcg cgaggttcaa
    53221 cgatcctggc gtaacggtcg ctgacgaata caccgacacc gtaaacgaga agacaatcag
    53281 cgtcagcatc gctttatcac cgagcagact gcgcaactct ttgataccca gattaaaaat
    53341 attgcgtaaa tggcgcatca tccctcctgt tttttcagca gcaggatgct taagcccatc
    53401 accagcggga tggctatcag caacgggata aaaagttgcc acaaatcagt cagatccagc
    53461 gctttcgaga acgttccgcg ggcgatagtc agaaaatgac tggtcgggta aacctcgccg
    53521 atccaacgtc caagcccttc cagcgaagct accggatcga tcatcccgga aaactgtgtc
    53581 gccgggatca atgtgataat cgccgttccg aaaatggcgg caatctggct tttcataaag
    53641 gtggagatca gcagccccat tccagtggca atgatgatat acagcagcgc cgccaaggtg
    53701 agcgtcagga aactgccttt atgcggtacg ccaaacacaa acaccgacag gccgcagagc
    53761 aggaaaaagt tcagcatccc cagcgcgatg tacggcagct gtttgccaag caaaaattcg
    53821 ctacgggtgg tgggggtcac gtaaaggttg ataatcgacc ctagctcttt ttcccgcacc
    53881 acgctaaggg cgcttagcat tgacgggatc atcatcagca gaagcgggat caccgccgga
    53941 acaatcgctg gcaggctttt tacgtccggg ttatagcgat aacgcgtctc aatgttcatc
    54001 agcccgcttt ggctggcggg tgtcgattgt cggctcgcca catcctgaag ccaactctgg
    54061 tgcattgcct gcacgtaacc ttttaccgtt tcggcacgac tgggcatcgc tccgtcgatc
    54121 cagacgccga gttccacagg cgtaccacgc gcgatatcgc gcccgaaatt gggcgggatc
    54181 tcaatggcca ccgtgatatc gcccgcacgc atccgacgat caagctcgtc ataactggtg
    54241 agcggcggct gttcgataaa gtaacgggaa ccggagaggt tgagtgtcca cgcctggcta
    54301 ctgacggtct ggtcgcggtc gagcaccgca aagcgcaggt tttccacatc catactgatg
    54361 ccgtaaccca ttatcagcat caggatcacc gttcccatca gcgccagcgt cgaacgtact
    54421 ggatcgcgtc gcagttccag cgcttcgcgg cggctgtagc taaacagacg gcgcaggcta
    54481 aatccctgac gcggcgcgtg ggtggtgtcg tgtaccacgg gcggcgcttc ggcttcgttg
    54541 ctctgccctg ccgcttcctg caaataggcg ataaatgcct cttccagact ggcggctccg
    54601 cgtttctcaa ccagttcctg cggtgtaccg ctggcaagca cttttccggc gtgcatcagt
    54661 gagatgcggt cgcaacgttc cgcttcgttc ataaagtggg tggagatgaa gatagtcact
    54721 ttgtcctggc gcgagagatc gaccatcaac tgccagaaca tatccctcgc caccggatcg
    54781 acaccagaag taggctcatc gaggattaac atctccgggc gatgaatcac cgccaccgcc
    54841 agcgaaagcc gctggcgaat gccgagcggc aatgactccg gcagaacatc ttcaacgtcg
    54901 ttgagcttaa aacgctcgct catttcagcc actcgcgcgg gaatttccgc ttccgggatg
    54961 tgaaacaaac gggcatgtaa ctccaggttt tgccgcacgg tgagttcgtt atagagcgaa
    55021 aacgcctgcg acatatagcc cacacgacgg cgggtatcga tatcttttgg atcaaccggt
    55081 tgcccaaata gccacgcctc accttcgctg gcgggcagca gtccggtaag cattttcatg
    55141 gtggtggatt taccgcagcc gttcgaacca aggaatccaa aaatctcccc gcgtggaatg
    55201 cggaaattga cgtgatcaac ggcaacgaag gaaccaaaac gcatggtcag atcgcgcgct
    55261 tcgatggcaa tctctgcgtt ttcaggttga tacggtggga tcactaccgc ctgatgcgcc
    55321 tggcgttgcg cttgcggtaa cagattaata aatgcttctt ccagcgtggc gctttgcgtt
    55381 tgctgccgca gctcttcggc gctgccggtt gccagcactt ctccggcatt catcgctacc
    55441 agccagtcga agcgttcggc ctcttccata taggcggtgg cgaccagcac gctcatattg
    55501 ctctgccgct ggcgaatact gtcgatcaga tcccagaact gggcgcggga gagcgggtca
    55561 acccccgttg ttggctcatc aaggatcaac agttccgggt cgtggattaa cgcgcagcac
    55621 agcccaagtt tttgcttcat cccgccggag agtttacctg ccggacgatc gcgaaacggt
    55681 gctaacccgg tgctggtcag cagctcatta attcgcactt cccgctccgc tttatcgtga
    55741 ccgaacaggc gagcgaaaaa atcgacattt tcatacaccg acaaggtgtg gtagaggttt
    55801 ttgcccagcc cctgcggcat ccaggcgatg cgcgggcaga cgtctcggcg atgcttcggg
    55861 tcgtgcatat cgccacccag caccaccaca ttgccctgct caatgacgcg ggcaccggaa
    55921 atcagtgaca ataaactcga cttcccgacg ccatccggac caatcagccc gaccatgcaa
    55981 cgggccggaa tatcgagagt gatattgttc agcgcaacgg tttttccata atgctggctc
    56041 acgcccgcca gttgcgcgac aggcgggacg ggaaccagtt ccagatgcgt cattgcggca
    56101 acctcaccac gaggtcgtca ggccacggaa gttcttcatt cacccgcacc cacgctacgc
    56161 ccggtaaacc ggttttgaca tattccagat gctgctggag taattccggt gggatacgcg
    56221 ctttgacgcg gaacatcagt ttcagccgtt catcgctggt ttcgacggtt tttggcgtga
    56281 actgggcgac actggcgaca aaactgatgg ttgcaggaat acgcagatct ggcgcggcat
    56341 cgaggatcag ccgggcttca ccgcccagtt tcagcgtgcc cgcctgttcg gttggcagga
    56401 agaaggtcat atagacgtcg ctgagatcga ccatattcag cacccgaccg cctgccgcca
    56461 gcacttcccc tggctcggca acccgatact gcacgcgtcc gtcacgcggg gctttcagtt
    56521 cactgtcatc gatatctgcg gcaatgcgcc gttcagtggc ttgcgctgct tcgacccgag
    56581 tttgcgcctg aatgatattg gtgcgtgctg cttctatagc cgctttagaa gccgaaacct
    56641 gggctttcgc tgattccagc gcagctcggg cgctctcagc ggcggcgcgg tcgtcatcca
    56701 gctgttgcgc agaaatagcc cctcgttggg cgagcgagcg ggaacgcgta tgacgtttgg
    56761 caacagagtc cagttcggcc tggcgttgat tgaccagcga ctgtgcggca cgagtttcac
    56821 tttgtcgttg ctccagcaaa gcctgcgcgg cggcaacggc gctttgtgcc tctttgattt
    56881 gcgcgatggc ttccagtcgc tgttcctgca acacgcgagt atccatcttc gccagcactt
    56941 cgccttcgcg aacaaactgg ccttctttca ccagaatggt gtcgatacgc ccggcaattt
    57001 tgctggcgat atccacttcc gtcgcttcaa tgcgcccatt gctgacagca aagccttccg
    57061 gcacacccgc cgggcgcaac agccaccacg ccacgacggc agccaccgcc agtgccccga
    57121 caacccacca cgccagatgg cgcttactct tatccataat cgacccgcca taatccctgt
    57181 aagcaaacga cgcgtgttac aactgccgcc gaacgacgtt cagcggattc ttccatccgg
    57241 gataagaagg aaacgacaaa tacgaccagc gcgcagagca ttgagcgcca acggggaagg
    57301 cttagtgaaa acgggtagta atgttgtcat catcagcatt cctggccgta aatgaaacac
    57361 gcatactcat aagtgtgatc tataaaactg gctgacaaaa cggtatccat cctgttggat
    57421 cgcatttcac gcctgttcag ttgcaggaac agagcattcc tttttacgtt ttatcaacag
    57481 tctcaaccag atagtaacat taatattcat gttgtctatg gttcagagct gtaacattgt
    57541 gcgccttcta atcaaaggca taagtccatt tctgtttttt tcatgctggt aagacttcca
    57601 gggagtggtt atctctggcc ctgcaccagg gttaatttgg caattatgca tgctgaacat
    57661 tcttccagca tgtttactgt cacgggcgtt aagacctaaa actgtccccg tttcacctat
    57721 gcgttctctc ccaccatttc cgttatgtgc aacaacttga tttataccta acatttgtga
    57781 acatgctaat agtgcttcaa gtttaggtct gtaatctgtc gaaacagaaa aataacgatt
    57841 aaaggatcga caagagatag cccggctagt tttaaacttt ttgccattaa ttatagcatg
    57901 atgcttttta ttaataattt ccgctaattc gaccggatcg agagcattgg tcatttcatc
    57961 aatagagaca gttatcggtc ctataatgta acgttttcgc tgatcatcaa aagttatccc
    58021 atgatgattt gccattattt tgtatttggg gtcatagaaa catacaggat aatcttttat
    58081 cccgtcgtag gtatcctcat caagtggttt catgcgcgtg aagttttgca tatagttacc
    58141 attgcaattc gtttcatgat ttccagctaa tacatgaaca ttttcattga tactcattat
    58201 gcgttttaac aaatcgatga taaattgatc gccacagata ttactaaatc gatcgccggt
    58261 ttggtcgcca agaaacgtac atggcgtgac tgcgtcttta ttaataacca aataaggctg
    58321 aagactttct aaaacattat ctcttctgtt cagtatactg attattgtat taacattagt
    58381 gtcaagttgc tcattaaagt tctcccttag caatacgttg agaacttcat cttctatatt
    58441 aagcaactgg ctaaggaaat tttctccttc agccatgatt cccaaatgtc ctgttgcaaa
    58501 aagcgcgtac aaaactgcac cgactgagcc atcggtatcg ccaaaatagg tcggcttggt
    58561 acttgtggga tcaacaggtg aacaattgga gaaataggtt ctgtcagcga actggggttt
    58621 tataattgtt tcgaatttaa tatcaaatac attgtattgc gatatttccc agttccagat
    58681 gggtttgtac gactcagtaa ctgcaacatc attagcatta tcatttactg tcatattatt
    58741 aagtaaataa acaatgctgc accaattaat atcggtatta tcaagagggc aaccgtaatc
    58801 tttactatta attgtcacaa taaactgata acagttattc ctacgtagaa tatgagcgcg
    58861 catgcccgat agcatactat atttttctga atgattcgtt tgtatggtaa atgaaatttt
    58921 gtcatcttga atagattgtt ggaaacgttc tatttgccaa tcatcaattt tttccacaac
    58981 ttcaccgagc attgtcctgg caatacttga tatatcatca cctataccat ttgcaatata
    59041 cccaaacagt tctttagtga agttggtaac atgctcttgc accatatcat ttgttgcgat
    59101 tgtcgttgta ctgccattag tgcctgccac agttccaatt ttcatctttt acctcatatt
    59161 attcaccgcg aggaatacat agttaacatt ttatcttatg acatacacct catattcgat
    59221 ggggaaataa ttatattttg cacaaatcat tcagccttaa agcataatat gaaatttctt
    59281 atctcatagc agagaaagtc cctaaagatt gtagaggcgt catcagtaca tacctctaca
    59341 atcgattaag aaaacctatg aaatactggc aaaattaaaa agagatacca cagcactcat
    59401 gaagttttat tgaggttgaa aacaactaag ccctgggaca ccttttttat caccacgttc
    59461 gttaggtgct atcttccaag aatttagccc catggtataa ttatcaattt tttgatgctg
    59521 gtctacccct aatccataat ggccaaattt ctgtttagtt tttttgagcg tctgtccaca
    59581 tttgaagata tcttcaggtt ttggtctgaa agcagtagtg ttattaaaac aattttcata
    59641 tacaggtgtg ggtgctatag cattgcttct aattagatgg agaagatgat catccatgtg
    59701 ttgtttatgt tttttattac atatatttgc taattcctca ggatcaattg ggtctttcat
    59761 ctgattaaat ggaacctgca aagcaccgag acaataacat tgattttctt catttcttat
    59821 tattccgtga tgactggtta aaactttagt ttctgagtta taattacaaa catcgagcgt
    59881 tttaattaag ttatacgtat caccggcaga gagtttataa ttttctagtc gtgcagtata
    59941 gttaccatta aaattgattt catgatttcc agccagaacg atgacatttt tattaatacg
    60001 gctatcttta ttaccctcca tatttcgcat agaatttagc aaagtaagga tatatttatc
    60061 accaaaaata gtactaaatc tatcgcctgt atgatcccca aggaagatac aaggggttat
    60121 cgcatcgtcc tttaaaaaaa catgttgatg caactcattc aatatcatgt ctctcgtatt
    60181 taataaattg tagagcaatt tattattttt atgtatgtat gcagatgaag ccatgtcttc
    60241 gtgttttaat aattcacata ataaactcca acccttttcc ctgatgccta tatgccctga
    60301 aacgagcaat gaataaagta cagcgcctac tcttccatct gtatcgccaa aataagccgt
    60361 gttggcattt tgtgggttaa tcacaccaca ttgtttataa aagtctattt tagaaaacac
    60421 agatttaaca tagctatcaa acttatcctt aggaaacgtc tctttagaga catcccaacg
    60481 ccagtcagct ttaaaattac cgctaagaag agtaaagaaa ttttcaggta tgatagaaag
    60541 cccattaact ttataaatta tgtctgattt atctccggag aaattaataa aataataacc
    60601 atccttacac tcaatcttaa ctgtaatggg agacgataat tttatcacat ggttattgtc
    60661 tggtataatt gtaaagataa acgtgttagt agtattatcg gcaaaactat tatattcttg
    60721 aatatctata gatggctcac tattgtaaaa ccatattttc tccatctgaa gactatgttc
    60781 atttaccctc cagtgaccat gttgcaggaa atagatgata tgcatgccaa aatcgttatc
    60841 tatttgcgaa tcattatcca gcttatcaat ttcaactatc tttgaacgat gtgttttatc
    60901 aaccttgcta tggtgttgat gattattaac tttatctgaa tgcttatctt tatggtttga
    60961 agggatgtac ttaaatatat tgtcaattgc tttcataaat aacccgcgat atttttgacc
    61021 tgttagtaaa aaataaatga tacatgtctg aaagacatat ttacgacgat ataaaacaat
    61081 aatacttata tgttcgctga ttttaagcac cgagactatc cggtctttta ctaaataatg
    61141 gaaaacaatc catccctgga agacaattgt aaaaaaaaac aacagaataa gaattaatct
    61201 taggataaat atttatttat catggccttt tgaccgctaa aaaatggcac tcggcacagt
    61261 aaaccccgct atagttatgc tatgcatttg tctttaatga tatctcgtcg ttatattaac
    61321 aggctgaaat tatcattaat gcacatttca atattagcag ggatacctga ggagtttaaa
    61381 gtgaacatat atatcgggta gcttttcaaa ttaatccctt tgattatagg cttaatttgc
    61441 atcgcgttag gtggctttgt gctggaaagt tcagggcaac gtcacgggtc atgtgctgat
    61501 ttctctggcg gccatatgcc tggcattatt cactaccgca tttattatca tttcgcaact
    61561 cacgcgcggc gttaatacgt tttacaatac attgttcccc attattggct atgcggggtc
    61621 aattatcacc atgatatggg gttgggcact gttagcaggt aatgatgtga tggcagacga
    61681 gtttgttgcc ggccatgtta tttttggcgt tggtatgatt gccgcatgtg tatcgacggt
    61741 ggcagcgtcg tccggtcact ttctgctcat tcccaaaaat gcagcgggga gcaagagcga
    61801 cggaacaccg gtacaggctt attcttcatt aatcggtaat tgcctcattg ccgttcccgt
    61861 tttactcacc ctgctcggtt tcatttggtc tattacgctg ttacatagtg ccgacataac
    61921 tccgcattat gtcgccttgg gttaaccgca atctgtgcct gtctaattgg cctagtggcc
    61981 acaatcgtcc atcaaacacg taatacgttc tcatccaaag agcactggct gtggtgttat
    62041 tgggttattt ttctcggctc agtcacggtg ctgcagggga tatacgtctt agtcagttcc
    62101 gatgcaagcg cccgactggc tcccggcatt attctcattt gcctcggaat gatctgttac
    62161 agcatattct caaaagtctg gctactggca ctggtctgga gacgtacctg ttcgttagcc
    62221 aacagaatac cgatgatacc ggtatttacc tgtctgtttt gcttgttttt gtcatcattc
    62281 ctcgcggaaa tcgcacaaac tgagctggac tactttattc catctcgggt tctggttgga
    62341 ttaggcgcag tatgttttac attgttctct attgtttcaa tattagaggc cggttctgcg
    62401 aaaaaaaatt aaaatttaca taatcccagc gttattcata tcttaacgct ggttttttac
    62461 gaacttagtg aaataatgta gaatataaaa ttataatttt atattggagc acatgtgtta
    62521 atgatcaacc ccgggatact aaaaatatag acattattaa acttgcttag cctcgcattg
    62581 cctttacctg ttaaagatgc atcgagaaaa ttgagtctga agttctcatc ctttatctga
    62641 ttttccagtc ccgaaacttt ggccttagca ttacctgaga tccgtccgtt actatctatt
    62701 ttgtaggtaa aaaaatacga atgggaaaat ttcctcggtg tatcttcatc agttaagcca
    62761 acaatcgtaa aactaccgct accatctttt tttaaaatga ctgaaaggtt agcagagtag
    62821 tataaagaat gatctgtttt agcatttctt gttaacgtcg acgtacattc aagagacgtt
    62881 gtgctaccat cacgagcctt ccagagagca aaaaaacaca cctgtagcaa tgtgaaaaca
    62941 agtattaata taaaatacat tttcttatta gtcatataat agcacctctg aacaaattgc
    63001 ataagtttcg tcattagagg attttataca tcttgataaa taccgctgcc cttccccaag
    63061 caaataatgc atactattta catcatatat aaaatttgca cctggcacac agggtaaatg
    63121 ctttttcgaa atttcagcgg cctctatttg agatttagcg aggccaatct cgttaagatt
    63181 ctctgaaaga tataaatcac aggtgtcaat cttacctgat ttaaccaatg gataatccgt
    63241 tatactgtag gtatctttaa aagccacggc aaatatttca ataataacaa ttactaccac
    63301 aagcgaaatc aaaatgcgaa aaagaggaat actccttttg cgctctactt tttcaacaac
    63361 atcttcttgt aaatgagaat catcataaga ttgtttactt attgttatgt tttcactgat
    63421 gtaaaagcct actttgggta tcgtaagaat cacattttct tcaacaccaa tatttttaaa
    63481 acttttgcgt aacagggata tatattggtt caatgtgttg tttgacagat caaatgaaaa
    63541 tctctccaaa acgctatcaa tgattgagtt cctgtcaacc gctccaccat tactttccag
    63601 taacactgcc aaaatacacc ccgccgtagc tggcaaattt atctcatcct gggtatttac
    63661 attcgagagc gagcatttgt tcggatcaaa aataatcgcg tcattgataa tgaaaaaaca
    63721 tctccgctcc atgcaggcaa caacacaaaa taagattttg tcattttagc ctattcctgg
    63781 tggaactgac acaagaaact aaaatacaaa tataaccctg aaaaagtcat ataaaactta
    63841 tatccacaga ataaaaaatc aaacatgtca gttttatcaa tataaagtat cgcaaggcga
    63901 aaagtgatgc aagaatactc ctaagtaaat gaaagcgcac cacatgaaat tatcaacaca
    63961 tgtaatttca tcattaggtg ctatcgaaaa taactcagga gctacttaag atgaataatg
    64021 tcttactcat gagacccgat gactcagaat ttatgatacc tgatgactgg tcagggctaa
    64081 acgcaaaagg cattacatac gaacaagata atagagtgac agagtctttt ctgcctttta
    64141 aatcttttta ttgttcaccg gtgtgcacga cagttgaact catctcacaa aataattgtt
    64201 ttgctatggg tatccgctac cttctggaaa agaaaagaga cttaaggcta aatcacttta
    64261 aagccttctc tcataaatgc gagacaaaac ctgacgtgtt aattatgacc gggtgcaccg
    64321 tcgctgatac ttatagtata ttctcacgca ttgttgaaga aaatataatg aaaacgatga
    64381 cacctatcgt catcattact gggcatcact cagaagatct ttgttctctg gcatcgaaac
    64441 tgggaggagt taaaataata tcaccgacag aaaagattgc aaaaatagaa aacgctatca
    64501 atgattcttt taacagcaag cctaaaaaaa taagattttt aacagatata ctctctcctc
    64561 aacaaattga gaccatgaaa atggttgcgc agggtagatc cgttaatgag atttcagccg
    64621 caatgaatat tagcatcaag accgtgtatg cacataaagt tcgtgctatc aataagctgg
    64681 gaattaagaa aaaagtttat gaagcgcttt tttataaagc gctaattcag ttcaactgat
    64741 gtaaaaatca ccctcgctta ataaataata tattctatgt ctttttttat aagttaaaaa
    64801 aggcgggata tcaaaccccg ccatgaaatt aactaatcat ctgcattatt ctgcgtagat
    64861 ataagcctgg gtacggatgg tgtaagagtc tggctcaaca acctgttctt tcatatggga
    64921 aacgacgcga taggcaccac ctgttccagt atttttcagc atcatataac gcttaccgcc
    64981 aatctcttcg gaggcagctg aaggaggatc ctgctcaagc gttgtcaggg aaactttcag
    65041 tttatggtcc gggttattgt caccagaaat tactgcatcc gctgaagtac tgctctccgc
    65101 ataaatatca tccgggaaag ttacagcata gtcgtggctc tgcccatcag atgcgccaac
    65161 aatacctgtt gccaaaggcg tccatgcagt taacgtaccg ctatataccc ctttcactgg
    65221 ttgaatggta ttattaacaa cgataggctt ggtcaaagta agattagctg acgcttcagt
    65281 accctggctt actaaagttc ccgcctgaga agcaaaagct gtccccgcca gcatgattgc
    65341 taacaatgtt ttcttcatta tattttctcc atgattttca tttacactgc aaaatgcagt
    65401 ttctttcttt gcatttacgt gctatcgcga aaatgcttcc accaaataat ggaaacatct
    65461 tcacaaaaat aaattatgag tttcttaatt tagaaactat aaacatgtcg ccgctatctg
    65521 gtataaccct gatgcacttt tcttcgcagg aagttggtat tttgcgatac aggattgatc
    65581 tttagaataa ccccatttca cattaaactc gcct
//
LOCUS       DAEEUE010000010        60377 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.010, whole genome shotgun sequence.
ACCESSION   DAEEUE010000010 DAEEUE010000000
VERSION     DAEEUE010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60377
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.010"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..316
                     /locus_tag="KLK84_000672"
     CDS             <1..316
                     /locus_tag="KLK84_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE2008128.1"
                     /translation="GTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSN
                     SVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVT
                     YP"
     gene            429..1223
                     /gene="panB"
                     /locus_tag="KLK84_000673"
     CDS             429..1223
                     /gene="panB"
                     /locus_tag="KLK84_000673"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBE2008129.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1235..2086
                     /gene="panC"
                     /locus_tag="KLK84_000674"
     CDS             1235..2086
                     /gene="panC"
                     /locus_tag="KLK84_000674"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBE2008130.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2160..3062)
                     /gene="rpnC"
                     /locus_tag="KLK84_000675"
     CDS             complement(2160..3062)
                     /gene="rpnC"
                     /locus_tag="KLK84_000675"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HBE2008131.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3336..3716
                     /gene="panD"
                     /locus_tag="KLK84_000676"
     CDS             3336..3716
                     /gene="panD"
                     /locus_tag="KLK84_000676"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HBE2008132.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3720..4949)
                     /locus_tag="KLK84_000677"
     CDS             complement(3720..4949)
                     /locus_tag="KLK84_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBE2008133.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5013..5453)
                     /locus_tag="KLK84_000678"
     CDS             complement(5013..5453)
                     /locus_tag="KLK84_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBE2008134.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5558..6328)
                     /locus_tag="KLK84_000679"
     CDS             complement(5558..6328)
                     /locus_tag="KLK84_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE2008135.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6325..7251)
                     /locus_tag="KLK84_000680"
     CDS             complement(6325..7251)
                     /locus_tag="KLK84_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE2008136.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STAIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7360..8022
                     /gene="can"
                     /locus_tag="KLK84_000681"
     CDS             7360..8022
                     /gene="can"
                     /locus_tag="KLK84_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HBE2008137.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8063..8599)
                     /gene="hpt"
                     /locus_tag="KLK84_000682"
     CDS             complement(8063..8599)
                     /gene="hpt"
                     /locus_tag="KLK84_000682"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBE2008138.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8805..11195
                     /gene="gcd"
                     /locus_tag="KLK84_000683"
     CDS             8805..11195
                     /gene="gcd"
                     /locus_tag="KLK84_000683"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HBE2008139.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11242..12792)
                     /gene="cueO"
                     /locus_tag="KLK84_000684"
     CDS             complement(11242..12792)
                     /gene="cueO"
                     /locus_tag="KLK84_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HBE2008140.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12958..13305
                     /locus_tag="KLK84_000685"
     CDS             12958..13305
                     /locus_tag="KLK84_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HBE2008141.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13411..14277
                     /gene="speE"
                     /locus_tag="KLK84_000686"
     CDS             13411..14277
                     /gene="speE"
                     /locus_tag="KLK84_000686"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBE2008142.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14293..15087
                     /gene="speD"
                     /locus_tag="KLK84_000687"
     CDS             14293..15087
                     /gene="speD"
                     /locus_tag="KLK84_000687"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBE2008143.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15125..15487)
                     /locus_tag="KLK84_000688"
     CDS             complement(15125..15487)
                     /locus_tag="KLK84_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HBE2008144.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15662..18259)
                     /gene="acnB"
                     /locus_tag="KLK84_000689"
     CDS             complement(15662..18259)
                     /gene="acnB"
                     /locus_tag="KLK84_000689"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HBE2008145.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18614..20467
                     /locus_tag="KLK84_000690"
                     /pseudo
     CDS             18614..20467
                     /locus_tag="KLK84_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20538..21962)
                     /gene="lpdA"
                     /locus_tag="KLK84_000691"
     CDS             complement(20538..21962)
                     /gene="lpdA"
                     /locus_tag="KLK84_000691"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBE2008146.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22287..24131)
                     /gene="aceF"
                     /locus_tag="KLK84_000692"
     CDS             complement(22287..24131)
                     /gene="aceF"
                     /locus_tag="KLK84_000692"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBE2008147.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAREFGVNLAKVKGT
                     GRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSKFGEIEEVELGRI
                     QKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLDVKITPVVFIMKA
                     VAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFKDVNKKGIIELSR
                     ELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEVAILGVSKSAMEP
                     VWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRLVM"
     gene            complement(24146..26809)
                     /gene="aceE"
                     /locus_tag="KLK84_000693"
     CDS             complement(24146..26809)
                     /gene="aceE"
                     /locus_tag="KLK84_000693"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HBE2008148.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(26970..27734)
                     /gene="pdhR"
                     /locus_tag="KLK84_000694"
     CDS             complement(26970..27734)
                     /gene="pdhR"
                     /locus_tag="KLK84_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HBE2008149.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28275..29648
                     /gene="aroP"
                     /locus_tag="KLK84_000695"
     CDS             28275..29648
                     /gene="aroP"
                     /locus_tag="KLK84_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HBE2008150.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWTMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29691..30545)
                     /gene="ampE"
                     /locus_tag="KLK84_000696"
     CDS             complement(29691..30545)
                     /gene="ampE"
                     /locus_tag="KLK84_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HBE2008151.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30542..31093)
                     /gene="ampD"
                     /locus_tag="KLK84_000697"
     CDS             complement(30542..31093)
                     /gene="ampD"
                     /locus_tag="KLK84_000697"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HBE2008152.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31181..32074
                     /gene="nadC"
                     /locus_tag="KLK84_000698"
     CDS             31181..32074
                     /gene="nadC"
                     /locus_tag="KLK84_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBE2008153.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32277..32717
                     /gene="ppdD"
                     /locus_tag="KLK84_000699"
     CDS             32277..32717
                     /gene="ppdD"
                     /locus_tag="KLK84_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HBE2008154.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32727..33874
                     /gene="tadA"
                     /locus_tag="KLK84_000700"
                     /pseudo
     CDS             32727..33874
                     /gene="tadA"
                     /locus_tag="KLK84_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33864..35066
                     /gene="hofC"
                     /locus_tag="KLK84_000701"
     CDS             33864..35066
                     /gene="hofC"
                     /locus_tag="KLK84_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HBE2008155.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35101..36144)
                     /gene="guaC"
                     /locus_tag="KLK84_000702"
     CDS             complement(35101..36144)
                     /gene="guaC"
                     /locus_tag="KLK84_000702"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBE2008156.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36306..36344)
                     /locus_tag="KLK84_000703"
     CDS             complement(36306..36344)
                     /locus_tag="KLK84_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2008157.1"
                     /translation="MIIKTLKMSARK"
     gene            36369..36989
                     /gene="coaE"
                     /locus_tag="KLK84_000704"
     CDS             36369..36989
                     /gene="coaE"
                     /locus_tag="KLK84_000704"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBE2008158.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            36989..37732
                     /gene="zapD"
                     /locus_tag="KLK84_000705"
     CDS             36989..37732
                     /gene="zapD"
                     /locus_tag="KLK84_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HBE2008159.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37742..37939
                     /gene="yacG"
                     /locus_tag="KLK84_000706"
     CDS             37742..37939
                     /gene="yacG"
                     /locus_tag="KLK84_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HBE2008160.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38039..38428)
                     /gene="mutT"
                     /locus_tag="KLK84_000707"
     CDS             complement(38039..38428)
                     /gene="mutT"
                     /locus_tag="KLK84_000707"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HBE2008161.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38488..41193)
                     /gene="secA"
                     /locus_tag="KLK84_000708"
     CDS             complement(38488..41193)
                     /gene="secA"
                     /locus_tag="KLK84_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBE2008162.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41255..41767)
                     /gene="secM"
                     /locus_tag="KLK84_000709"
     CDS             complement(41255..41767)
                     /gene="secM"
                     /locus_tag="KLK84_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HBE2008163.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(41998..42915)
                     /gene="lpxC"
                     /locus_tag="KLK84_000710"
     CDS             complement(41998..42915)
                     /gene="lpxC"
                     /locus_tag="KLK84_000710"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HBE2008164.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43016..44167)
                     /gene="ftsZ"
                     /locus_tag="KLK84_000711"
     CDS             complement(43016..44167)
                     /gene="ftsZ"
                     /locus_tag="KLK84_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBE2008165.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44228..45490)
                     /gene="ftsA"
                     /locus_tag="KLK84_000712"
     CDS             complement(44228..45490)
                     /gene="ftsA"
                     /locus_tag="KLK84_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBE2008166.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45487..46317)
                     /gene="ftsQ"
                     /locus_tag="KLK84_000713"
     CDS             complement(45487..46317)
                     /gene="ftsQ"
                     /locus_tag="KLK84_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HBE2008167.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46319..47239)
                     /gene="ddlB"
                     /locus_tag="KLK84_000714"
     CDS             complement(46319..47239)
                     /gene="ddlB"
                     /locus_tag="KLK84_000714"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBE2008168.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPIDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47232..48707)
                     /gene="murC"
                     /locus_tag="KLK84_000715"
     CDS             complement(47232..48707)
                     /gene="murC"
                     /locus_tag="KLK84_000715"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBE2008169.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48761..49828)
                     /gene="murG"
                     /locus_tag="KLK84_000716"
     CDS             complement(48761..49828)
                     /gene="murG"
                     /locus_tag="KLK84_000716"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBE2008170.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49825..51069)
                     /gene="ftsW"
                     /locus_tag="KLK84_000717"
     CDS             complement(49825..51069)
                     /gene="ftsW"
                     /locus_tag="KLK84_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HBE2008171.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51069..52385)
                     /gene="murD"
                     /locus_tag="KLK84_000718"
     CDS             complement(51069..52385)
                     /gene="murD"
                     /locus_tag="KLK84_000718"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBE2008172.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52388..53470)
                     /gene="mraY"
                     /locus_tag="KLK84_000719"
     CDS             complement(52388..53470)
                     /gene="mraY"
                     /locus_tag="KLK84_000719"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBE2008173.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53464..54822)
                     /gene="murF"
                     /locus_tag="KLK84_000720"
     CDS             complement(53464..54822)
                     /gene="murF"
                     /locus_tag="KLK84_000720"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBE2008174.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54819..56306)
                     /gene="murE"
                     /locus_tag="KLK84_000721"
     CDS             complement(54819..56306)
                     /gene="murE"
                     /locus_tag="KLK84_000721"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBE2008175.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSCVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56293..58059)
                     /gene="ftsI"
                     /locus_tag="KLK84_000722"
     CDS             complement(56293..58059)
                     /gene="ftsI"
                     /locus_tag="KLK84_000722"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HBE2008176.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKESMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58075..58440)
                     /gene="ftsL"
                     /locus_tag="KLK84_000723"
     CDS             complement(58075..58440)
                     /gene="ftsL"
                     /locus_tag="KLK84_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBE2008177.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58437..59378)
                     /gene="rsmH"
                     /locus_tag="KLK84_000724"
     CDS             complement(58437..59378)
                     /gene="rsmH"
                     /locus_tag="KLK84_000724"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBE2008178.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59380..59838)
                     /gene="mraZ"
                     /locus_tag="KLK84_000725"
     CDS             complement(59380..59838)
                     /gene="mraZ"
                     /locus_tag="KLK84_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBE2008179.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14469 a        16718 c        14834 g        14356 t
ORIGIN      
        1 tggtactgaa aacgggcaac tccttggtaa tactctcaca ggtaatgatg cagcaaaagg
       61 agtcggtgta ctcatagaag gtttagcaac tagtaaaaat cctctaatga cattgaaacc
      121 taatgattca aattctgttt ataaagatta cgacccgaga ggcaaagacg atacaacagg
      181 aggagtttac ccggatcaag atacaggtat aacataccct ctccatttcc aggccacgct
      241 acaacaggat ggaactatac caatagaagc tggtgagttt aaagccacca gtactttcca
      301 ggtaacctac ccttaataag tccaccgcac cgccatcatc tggcggtgcg gtaattgata
      361 aatcattgcc cccaccaatg tgactgataa aatgccaggc aacacgacat catttatcag
      421 gatacgttat gaaaccgacc accattgctt cacttcagaa atataagcag gataaaaagc
      481 gtttcgcgac catcaccgct tacgactaca gcttcgcgaa actatttgct gatgaagggc
      541 ttaacgtcat gctggtaggc gactcgctgg gcatgaccgt tcagggccac gattccactc
      601 tgccagtaac cgttgctgat atcgcctacc acactgccgc cgtacgtcgc ggcgcaccaa
      661 actgcctgct attggctgac ctgccgttta tggcgtatgc cacgccggaa caagcctttg
      721 aaaacgccgc aacggttatg cgtgccggtg ccaatatggt caaaattgag ggcggtgagt
      781 ggctggtcga aaccgtaaaa atgctgaccg aacgtgccgt tcctgtatgt ggtcacttag
      841 gtttaacccc acagtcagtg aatattttcg gtggctacaa agtacagggg cgcggcaatg
      901 aagcgagcga tcgactactc agcgatgcat tagccttaga agccgcgggg gcacagctgc
      961 tggtgctgga atgcgtgccg gttgagctgg caaaacgtat taccgaagca ctggcgatcc
     1021 cggttattgg tattggtgca ggcaacgtca ctgacgggca aatcctcgtg atgcacgacg
     1081 cctttggtat taccggcggt cacattccta aattcgctaa aaatttcctc gccgaaacgg
     1141 gcgacatccg cgcggctgtg cggcagtata tggctgaagt ggagtccggc gtttatccgg
     1201 gcgaagaaca cagtttccat taaggagtca cgttgtgtta attatcgaaa ccctgccgct
     1261 gctgcgtcag caaattcgcc gcctgcgtat ggaaggcaag cgcgtggcgc tggtgcctac
     1321 tatgggtaat ctgcacaatg gccatatgaa gctggtcgac gaagccaaag cccgcgccga
     1381 tgtggtcgtc gtcagtattt tcgttaaccc gatgcagttc gaccgcccgg aagatctggc
     1441 acgttatcca cggaccttgc aggaggactg cgagaagcta aacaaacgta aagtggattt
     1501 agttttcgcc ccttcggtaa aagagatcta cccgaacggt actgaaaccc acacttacgt
     1561 tgacgttcct ggcctttcga ccatgctgga aggtgccagc cgtccgggac attttcgcgg
     1621 cgtttcgact attgtcagca agctgttcaa cctggtccag ccggacatcg cctgcttcgg
     1681 tgaaaaagat tttcagcaac tggcgttgat ccgcaaaatg gttgccgata tgggcttcga
     1741 tattgagatt gtcggtgtgc caattatgcg cgccaaagac ggtctggcgc taagttcccg
     1801 taacggttat ctgacggcgg aacaacgcaa aattgcgcct ggtctgtaca aagttttaag
     1861 ttcgattgct gacaaattgc aggctgggga acgggatctc gatgaaatta ttaccattgc
     1921 ggggcaagaa ctgaatgaaa aaggcttccg cgccgatgat attcagattc gcgatgccga
     1981 cacattgctg gaagtttctg aaaccagcaa acgggcagta attctggtag ccgcctggct
     2041 tggcgatgct cgcctgatcg acaacaaaat ggtcgagctg gcgtaatact taactggcgc
     2101 tacggctgat ggcgccagtt attaatttac cccacgtcat ctgtcataac acaggtaact
     2161 taaataaggt taattacctt atcaatctca gcaagaggta aatttgccat ctctgcaacg
     2221 tcttcccgag acattccttt acttagaaga cgcagggcga attcttgcct ttcctcctgt
     2281 cttccctgct gaatcccctt ctcaatccct ttctcttcaa accactgcgc cagcgtcatc
     2341 atagacttcc ctcccgtttc cctgtctctc agcacaccat aaaacaaatc cgcttgttca
     2401 gtatgaccgc gttgcagcat atagttttgc atggcaacta actgacttcc gctagtgtac
     2461 ccttcgtcaa tcagcgtgac cagttgctcc agcaataaca ttaagtcgcg ctggcgaata
     2521 tgtttttgca gtagttcgag aatcgcaatc cgccgatgtt gcatgatttc gtcatccggt
     2581 gtgatggtga tatccaccag cgggaaagga ctgttataga cgcgtcgcgc cagctccggc
     2641 gagtaaaaca tatcaaacca gcacattgag agcggataag gtgtggcctc gccctgataa
     2701 aacagtatcg gcaccaccag cggcagctta tcgtggccag cctccagatg ccggtgcatg
     2761 gcagcaatag aataacgcat catgcgaaag gccattttct tatccggctt gctttggtgt
     2821 tcaatcacaa catgcagata accgggattg ccctgcattt gcatggaata gagcacgtcc
     2881 gtgctgtgac ctttcaggca ctcttcaatg aaactccccg actctaaatg aagcgtgttg
     2941 agatcacaaa gttcgcgtaa ttccactggc aaatgtatct ccagaaagtc gcgagccgtc
     3001 tccgcatgca ttaaaaattg tttaaatacc gcgtcatgcg gtgtggtact cggtgcatcc
     3061 atcttccctg tcttccgtgt ggccttgcgt gaaggcagta tccagtcaag gcgtgctatc
     3121 gtcagccacc tctttattgt ttaccgagca gcgttcataa aaatatcaat tcgttacagg
     3181 cgttacatgg cacgcttcgg cgcggtacgc aaactgctga cgcaaatgcc atcagatgag
     3241 tagacactca tcaaaaatcg ggcaatactg cgtgagaatt tcctcaaagc aggcaccgcc
     3301 tgcttcgtta acgacagggt agaaaggtag aagttatgat tcgcacgatg ctgcagggca
     3361 aactccaccg cgtgaaagtg actcatgcgg acctgcacta tgaaggttct tgcgccattg
     3421 accaggattt tcttgacgca gccggtattc tcgaaaacga agccatcgat atctggaatg
     3481 tcaccaacgg caagcgtttc tccacttatg ccatcgcggc agaacgcggt tcgagaatta
     3541 tttctgttaa cggtgcggcg gcccactgcg ccagcgtcgg cgatattgtc atcatcgcca
     3601 gcttcgttac catgccagat gaagaagctc gcacctggcg acccaatgtc gcctattttg
     3661 aaggcgacaa tgaaatgaaa cgtaccgcga aagcgattcc ggtacaggtt gcttgattgt
     3721 tatccctgcg gctggttact caccagccgc gacatcttct ccagcgaatc cgttcttaag
     3781 atataaagtc gttttagtaa caacggatta tcccccggtt ttactttgcc tttcatggtt
     3841 gtcaccgcca ggtgaaatcc ggcatcgttt gctgccttca cggctttgtc attaaagccg
     3901 ccaaacggat acgaaagata ccagacatgt ggattaaatt gcgccagagc gcggcgtgaa
     3961 cgtgcaaaat caaacagaat attgtgctca ctacggctca gtaatatggg tcggcgataa
     4021 ccatctaccc gatgcaaaaa atgggtatgt gactggaaat caaaaacatc gcgaatttcg
     4081 ttaagctcag aaacgctcat aaattgcagc gattttgggt tccacttctg cgggtgacgt
     4141 ttgatgcgtg aggtaacaat aaacaccgtc gccttcatgc catattgttt caacacagga
     4201 tacgcatagc ggctcaccga cttgaggcca tcatcaaagg taatcaccac cgctcgcgca
     4261 gggagattga tcgtattctt cacgtaacct tccagctgag ccatgctcag tgtcgcgtat
     4321 cccctgtcgc gcagccaggc catttggtta ttgaaagcgc gtaccgaggt ggtcgtcgaa
     4381 gtatggcgaa aacgggtgtt ttcttcgtcg cgcagaatat ggtgataagt tagcaccggc
     4441 aggccattat cgggttgggc atccagcgcg ctgatatagg ccagtcgatc gccaatacgg
     4501 atctgatacc aggtttgatt taacctgtct ttcagtttat gcaaaatcgg gtagcgcaaa
     4561 ttgtccgcca gtaccccaaa tggcgcactg cccacactcg gcgcgttgta gaccggcgta
     4621 tctttccagg taattaagtt ctgattactc agcggcttgt tgaggtcacc caaaccgtct
     4681 tcaacttttt gtcgcccctg aactggctcg agatgccctt tatcgataaa ccctttacca
     4741 aagccaaaat taaatgcgta ataacttgcg gcagtgggct ccacggcaat gatttgtccg
     4801 gcccgaatat tccccacggt caccatcttg tcactaattt gcgcccagac cgcagcattt
     4861 tcgatggttt gcatataacg ggcaggtaac gcggcactga cacttgcggt aaacagcatc
     4921 agcaggagaa taacagcttg tttatacatg aaaaaataac cgagggtgaa acaacgcttt
     4981 tattgtagca aaagcgccat cagaatcgcg ggttaatact catacaaatt atgtttgagg
     5041 acgaaaggcg gatttttttg ttgttggtgc caaagactac tcacctccgg cccacccagt
     5101 tcgacaatac actcacgaaa ctctgaactg gtcatggttt cacggcaagc catcatctgt
     5161 tcaattaacg gtaacgtgac accagaaatc acttcgcaac gggagtgttt gtggcttaat
     5221 aatgaagcca cgcgatacgg tggcgctccg gctatatcgg ttaagaagat gacaccctca
     5281 ccagagtccg cttcatgcag agcatcgcac atcatgcggc tgagcatatt agagcttaat
     5341 ccgcgccaga aattcacggc ccggcactga agaagtgccc catgtttttt ctccagcgca
     5401 tccagtatct cttgcgcccg gtcatcgtga caggtaatta cccaacctaa catcgccttc
     5461 ctccttagcc gaaaatcagc ctaccctgac cattgttgat aaagggtgat agccgtcaaa
     5521 actcctctcc cccgaatcca gaggagagga aatagcctta gctacgcaaa ccacgtccac
     5581 gttggatcag cgaccaacag atcaaataaa acgccacaat aaagaccacc agtacgccaa
     5641 aggtagtgac cagcggcaca tcatttatac cgaggaagcc gtagcggaaa ccactgatca
     5701 tataaacgat tgggttcagg tgcgacagcc cttgccagaa cggcggcaac aaagtcagtg
     5761 agtaaaagac cccgcccaaa tacgtgagtg gcgttaacac aaaggttggc accaggctga
     5821 tgtcatcgaa cgttttggca aacacgccgt tcagcaaacc cgcaagggag aatagcaccg
     5881 ccgtgagcac caaggttaag gcaacgaata cccacgaatg cacctgaaac ggcacaaaaa
     5941 acagtgaaat tgccgtcacc agaatgccaa caaacagacc acgcgccacg ccaccgccga
     6001 catatccggc aataatgacg tgagtcggaa ccggcgctac cagcagctct tcaatattac
     6061 gctggaactt ggcaccaaaa aatgatgacg cgacgttagc gtaggcgttg gtgatcaccg
     6121 acatcatgat cagccccggt acgatgaact gcatatagct gaagccatgc atatcgccaa
     6181 tacgcgaacc tatcaggtta ccgaagataa taaagtaaag ggtcatggtg atgactggcg
     6241 gcaccagcgt ctgcacccag atacgcataa agcgatggat ctctttcgcc cagatgcttt
     6301 ttagcgccac ccagtaaaga tgcatcatgc gcgatctcct tgtttttcat taaccagtga
     6361 aacaaacagc tcttccagac ggttagcttt gttacgcata cttaatacct gaatgccctg
     6421 ctcacttaac tgcgtaaata cgctgttgat cccctgctca cgtagcactt caacttccag
     6481 cgtcgcggta tcgaccagtc gatactgata gccatcgagc ttcggtaacg gacttttcgg
     6541 tgcgagatcg agaataaagg tttccgattt cagcttcgcc agcagcgcct tcatcgaggt
     6601 attttccacc agctcaccat gttgaataat gccgatattg cggcacagca tttctgcttc
     6661 ttccaggtag tgtgtggtaa gaatgatggt ggtgcctttg tcgtttaaat ccttcaaaaa
     6721 gccccacatt gagcggcgaa gttcaatatc cacgcctgcg gtcggttcgt cgagaatcag
     6781 tagtttaggt tcatgcatta acgcacgggc aatcattaaa cggcgcttca tcccgccaga
     6841 taacatacgc gcacgttcgt tgcgttttcc ccataaatcg agctgcttaa gatatttttc
     6901 gctgcggatg tacgcctctt tgcgctccac accgtagtac cctgcctgat tcaccacaat
     6961 ttgctgaacg gtttcaaacg ggttgaagtt aaattcctgc ggcaccagtc ccaactgacg
     7021 tttagcgttc acgacatctt tctcgagatc gtaaccaaat acgctgaccc gcccggaggt
     7081 tttatttacc agagagctga taataccgat agcggtcgat ttcccggccc cgttcggccc
     7141 gagaagcgca taaaaatcac ccgcttcgac ctgcaaatct attccacgaa gcgcctgaac
     7201 gccgcctgga tagttttttt taagctgttg aagttccagt gcaatggtca taaattttta
     7261 cttaccttac gttcttacac tttatctgtg gtttaaatcg tcccggagtt gccctatatt
     7321 agccaaacgt aattatttgg ttacaggtcg ttaacctcca tgaaagacat agatacactc
     7381 atcagcaaca atgcactatg gtcaaaaatg ctggtggaag aggatcccgg gttttttgag
     7441 aaactggcac aagcgcaaaa accgcgcttt ctatggattg gatgttccga cagtcgcgtt
     7501 cctgcagaac gtttaaccgg tcttgagccg ggcgaactct ttgttcaccg taatgttgct
     7561 aacctagtca ttcacaccga cctgaactgc ctttccgtgg ttcagtatgc agtggatgta
     7621 ctcgaagttg aacacattat tatctgtggc cactacggtt gcggcggcgt acaagccgcc
     7681 gttgaaaacc cggaactggg gcttatcaac aactggctgc tgcatatccg cgatatctgg
     7741 ttcaaacata gctcattgct cggcgaaatg ccgcaagagc gccgtctgga taccttgtgt
     7801 gaactgaacg tcatggaaca ggtgtataac ctgggccact ccaccattat gcaatcagcg
     7861 tggaaacgcg ggcagaaagt taccattcac ggctgggcct acggcattca cgacggcttg
     7921 ctgcgtgatc tggatgttac cgccaccaac cgcgaaaccc ttgagcaacg ttaccgtcac
     7981 gggatttcca acctcaagct gaaacacgcc aaccacaaat aaaaatgcca tgccggatgc
     8041 aacacatccg gcaacttcac acttactcgt ccagcagaat cactttgcca atatacggca
     8101 gatgacggta acgctgtgcg taatcaatgc cgtaacccac cacaaactca tccgggatcg
     8161 agaaaccgat aaattcaacc gggacgttca cttcacgacg ggacggttta tccagcagcg
     8221 tacaaatcgc caacgacttc ggttcgcgca ggcttaagat ctcacgcact ttcgacagtg
     8281 tattccccga gtcgatgata tcttcaacaa tcagcacgtc cttgccacgg atatcttcat
     8341 ccagatcttt gaggattttc acatcacggg tggtggacat gccgctaccg tagctggagg
     8401 cggtcataaa gtcgacttca tgagatacct gaacttcacg gcacaggtcc gccataaaca
     8461 taaatgagcc acgcagcaga cccaccagca ccatatcgct gccgctgtct ttgtaacgct
     8521 cagtaatctg acgacccagt tcggcgatac gcgctttaat ctccgcttcg gggatcatta
     8581 cttctacagt atgtttcata tctctaacca tatgatttaa aaacaaatca cttcgcacgg
     8641 gcaggttttg gcaccgcaac aaagcgcaag acgcgcagta taccagtaaa actattcatt
     8701 cgcggaatct gttaataaag ctcgaattgt gatgacggtc acacatgtta aaacctataa
     8761 ttaattgcta ctaaaatatt aatgaattga aatggtgtct ctttatggca attaacaata
     8821 cagcctcgcg acgattactc gtcacgctaa cagccctttt tgcagcgctt tgcgggctgt
     8881 atctactcat tggcggaggc tggctggtcg cgattggcgg ctcctggtac taccctatcg
     8941 ctggccttgt gatgctcggc gtcgcctgga tgctgtggcg cagtaaacgc gccgcgcttt
     9001 ggctatacgc agccctgctg ctcggcacca tgatttgggg cgtctgggaa gttggtttcg
     9061 acttctgggc gctgactccg cgcagcgata ttctggtctt cttcggcatc tggctgatcc
     9121 tgccgtttgt ctggcgtcgc ctggtcattc ctgccagcgg cgcagttgcc gcactggtgg
     9181 tcgcactgct gattagcggt ggtatcctga cctgggccgg atttaacgat ccgcaggaga
     9241 tcaacggcac cttaagcgcc gatgccacac ctgccgaagc tatctccccc gttgccgatc
     9301 aggactggcc tgcgtatggc cgcaatcagg aaggtcaacg cttttcacca ctgaagcaaa
     9361 ttcacgccga taacgtccac aagctgaaag aagcctgggt gttccgtact ggcgatgtga
     9421 agcagccgaa cgatccgggt gaaatcacca atgaagtgac gccgattaaa gtgggcgata
     9481 ccctttacct gtgtaccgct caccagcgcc tgtttgcgct tgatgccgcc agcggcaaag
     9541 agaaatggca ttacgatcct gagctgaaaa ccaacgagtc tttccagcac gtaacctgcc
     9601 gtggcgtctc ttatcatgaa gccaaagcag aaacggcttc gccggaagtg atggcggatt
     9661 gcccgcgtcg tatcattctt ccagtgaatg atggtcgcct gattgcgatt aacgctgaaa
     9721 acggcaaact gtgcgaaacc ttcgccaata aaggcgtgct caatctgcaa agcaatatgc
     9781 cagacaccaa accgggtctg tatgagccga cttctccgcc aattatcacc gataaaacca
     9841 tcgtgatggc cggttcagtc accgataact tctcaacccg cgaaacgtct ggcgtgatcc
     9901 gtggttttga tgtcaacacc ggggagctgc tgtgggcttt tgatccgggc gcgaaagatc
     9961 cgaacgcaat cccgtcagac gaacacacct ttacctttaa ctcaccaaac tcgtgggcac
    10021 cagcggccta tgacgcgaag ctggatctgg tctatctgcc gatgggcgtg accacgccgg
    10081 atatctgggg cggtaaccgc acaccggaac aggaacgtta tgccagctcg attctggcgc
    10141 tgaatgccac taccgggaaa ctggcgtgga gctaccagac cgttcaccac gacctgtggg
    10201 acatggatct tccggcacag ccgacgctgg cggacatcac cgttaatggt cagaaagtgc
    10261 cgattattta cgctccggcg aaaaccggta acatttttgt gctcgatcgt cgtaatggcg
    10321 aactggtggt tccggccccg gaaaaaccgg ttccccaagg tgctgccaaa ggcgattacg
    10381 ttacccctac tcaaccattc tcggaactga gcttccgtcc gacgaaagat ttgagcggtg
    10441 cggatatgtg gggagccacc atgtttgacc aactggtgtg ccgcgtgatg ttccaccaga
    10501 tgcgctatga aggcattttc acgccgccat ctgaacaggg tacgctggtc ttcccgggta
    10561 acctggggat gttcgaatgg ggcgggattt ccgttgatcc aaatcgtgaa gtggcaattg
    10621 ccaacccaat ggcactgccg tttgtttcga aactgctccc gcgtggtcct ggcaacccga
    10681 tggagcagcc gaaagatgcc aaaggcacgg gtacggaatc cggcattcag ccacagtacg
    10741 gtgtaccgta tggtgtcacg ctcaacccgt tcctctcacc atttggtctg ccatgtaaac
    10801 agccagcatg gggttatatc tcggcgctgg atctgaaaac taatgaagtg gtgtggaaga
    10861 aacgtattgg tacgccgcag gacagtatgc cgttcccgat gccggttccg gtgccgttca
    10921 atatgggtat gccgatgctg ggcgggccaa tctccacggc gggtaacgtg ctgtttatcg
    10981 ccgctacggc agataactac ctgcgcgctt acaacatgag caacggtgaa aaactgtggc
    11041 agggtcgttt accggcgggt ggtcaggcaa caccgatgac ctatgaagtg aatggcaagc
    11101 agtacgtggt gatctccgca ggcggtcacg gttcatttgg tacgaagatg ggcgactata
    11161 ttgtggctta tgccctgccg gatgatgtga aataaccata atgcccggag agatccgggc
    11221 atatttccga atacggtctt tttataccgt aaaccctaac atcatccccg tatcttcatg
    11281 ctccagcaga tggcagtgcg ccatataagc atgttctttc ggtgcgttgt gattaaactt
    11341 caccagcact tcactgacat tgccttctac cttaacggta tctttccagc ccgcgcgatg
    11401 agccgctggc ggtttgccat tttctgacaa gatacggaac tgcgtgccgt gaatatggaa
    11461 cggatgcagc atcatgtcgc ccacgccaga gataacccaa cgttcgtact gccctttcgc
    11521 cgccgcaaac atcggcttat tcatatcaaa cgcctgaccg ttgattttat tggcatggtg
    11581 gaaatcgaac ttcccgccgt ggttcatatg attcatattg ccgtgcccca tatggcccat
    11641 catctggctg tgatccattc cggccatcgc ctgatcgcca tatttctcca ttagcatctg
    11701 catccccatc atatcgagca tcgggtccat agaaagttgc agcttgcgta ccgtcagccc
    11761 ttccagcgaa ggtaacgcag gcaggctact taatgtgtca ggcaaagcac cggaggcact
    11821 aatagcaatc ggctgaatcc gcattaccgg atgcggcttg tcaaacggtg caatcgccat
    11881 ccccatctgg ctgaccggca gcgtcaccag gtcaaagggt ttgttgtcgt taacctccac
    11941 cagcacttca aaacgctcgc ccatcagcac cggcagctcg ctcaccttca ccggttcagg
    12001 tagcagacca ccgtcgctgg caatcacata cagcgggcga ttgtcgctgg tggcgaaatt
    12061 gagcgagcgg gcattacagc cattgagcaa acgcaggcgc agccaaccac gcggggcagc
    12121 gtgttgtggg tagattgcac cgttggtcag cagcgtatcg ccaaaccagc ccacggcggc
    12181 ggtcatcaca tccagttgat aatcaatctg cccgtcggcg ctaaatttct tatcctgaac
    12241 gatcaccgga acatcatcga taccccactg ttttggcagc attaatttca ggatctcgtc
    12301 atcttcaatc accaccagac cagccagccc catcgccacc tgtcgcccgg tcttgccatg
    12361 ttgatgtgga tggaaccagc aggtagcggc aggttgatca acgttcaacg tcaccgagcg
    12421 cttgccacct ggcggaataa ttccctgtgg gccgccgtcg acgtcacccg gtacttccag
    12481 cccgtgccag tgcaacgtcg tctcttccgc cagttggttg tagatatcaa ccgttaccgc
    12541 tttgccgcgc tgtaatttca ccgccggccc cagcagattg ccgttatagc cccaggtagt
    12601 tgcagttttc tcgccaaagg tggactgacc tgcgccaata gttaactgaa tgcgattacg
    12661 ggcatcggtc gtgagcaaat cagggatcgg taacgttggg cgttccgccg caaagactgc
    12721 acggctccac agtggcaagg ctgaagccac acccagtgcg acggaatatt tcaagaaatc
    12781 acgacgttgc atagttattt ccttattctt aagcaggcga aacaaaatca aacgttgagc
    12841 atagtccttc cccttacggg aaggtcaagc cgcagacaca atttaatcga caataatggt
    12901 cgtcgcctcg ctcgcagtgt gctaacgttt atcttcttta agcccctggt agaagcaatg
    12961 aagacgtttt tcagaacagt gttattcggc agcctgatcg ccgtttgcgc aaacagttac
    13021 gcgctcagcg agtctgaagc cgaagatatg gccgatttaa cggcagtttt tgtctttctg
    13081 aagaacgatt gtggttacca gaacttatct aacgggcaaa ttcgtcgcgc actggtcttt
    13141 ttcgcccagc aaaaccagtg ggatctcagt aattacgaca ccttcgacat gaaagctctc
    13201 ggtgaagaca gctaccgcga tctcagcggc attggcattc ccgtcgctaa aaaatgcaaa
    13261 gccctggccc gcgattcctt aagcctgctt gcctacgtca aataatccct gatacctttt
    13321 tgtagaaata ttgaccgtgc atccgcggtc aatgttagct attatgttgc gccctttttt
    13381 tacgggtgtt aacaaaggag gtatcaaccc atggccgaaa aaaaacagtg gcatgaaacg
    13441 ctacacgacc agtttgggca gtactttgcg gtagataacg ttctgtatca tgaaaagacc
    13501 gatcaccagg atctgatcat ttttgagaac gctgcatttg gtcgcgtaat ggcgctggat
    13561 ggcgtagtac aaaccaccga gcgcgacgag tttatctatc atgagatgat gacccatgtt
    13621 ccgctactgg cccacggtca cgcgaaacat gtgctgatta tcggcggcgg cgacggtgcc
    13681 atgctgcgtg aagtaacccg acataaaaac gttgagtcaa tcacgatggt ggaaatcgat
    13741 gcgggtgtcg tgtcgttctg ccgtcagtat ctgcccaacc ataacgccgg tagctacgac
    13801 gatccgcgct ttcaactagt gatcgacgat ggcgtcaatt tcgttaatca aaccagccag
    13861 acctttgatg tcattatctc cgactgcacc gatcctatcg gtcccggcga aagcctgttc
    13921 acctcagcat tttatgaagg ctgcaaacgt tgcctgaatc ctggcggtat cttcgtcgca
    13981 caaaacggcg tctgcttttt acagcaggaa gaagccatcg acagccatcg caaactcagc
    14041 cattacttca gcgacgttgg cttttatcag gcggcgatcc cgacctatta cggcggtatc
    14101 atgacttttg catgggcgac agataacgac gccttacgcc atctctcaac cgaaattatt
    14161 caggcgcgtt ttctcgcttc tggcctgaaa tgccgttatt acaatccggc aatccatacg
    14221 gcagcttttg ccttacctca gtatctgcaa gacgcactgg cttcacagcc gtcctaagga
    14281 gaagataaga aattgaaaaa actgaaactg catggcttta ataatctgac caaaagtctg
    14341 agtttttgta tttacgatat ctgctacgcc aaaactgccg aagagcgcga cggttatatt
    14401 gcttatatcg atgaactcta taatgccaac cgtctgaccg aaatcctgtc agaaacctgt
    14461 tccattatcg gggccaatat tcttaacatc gcccgccagg attacgaacc acagggtgcc
    14521 agcgtcacta ttctggtgag tgaagaaccg gttgacccga aactcatcga caaaacagaa
    14581 caccccggcc cactgccaga aacggtggtt gctcatctcg ataaaagtca tatttgcgta
    14641 catacctacc cggaaagtca tcctgaaggc ggtttatgta ccttccgcgc cgatattgaa
    14701 gtctctacct gcggcgtgat ttctccgctg aaggcgctga attacctgat ccaccagctt
    14761 gagtccgata tcgtaaccat tgattatcgc gtgcgcggtt ttactcgcga cattaacggt
    14821 atgaagcact ttatcgacca tgagattaat tcgattcaga actttatgtc tgacgatatg
    14881 aaggcgctgt atgacatggt ggatgtaaac gtctatcagg aaaatatctt ccataccaag
    14941 atgttgctta aagagttcga ccttaagcac tacatgttcc acaccaaacc ggaagattta
    15001 accgacagcg agcgccagga aattaccgct gcgctgtgga aagaaatgcg cgagatttat
    15061 tacgggcgca atatgccagc tgtttaacgg ctctggcgga gctcccaggc tccgccagat
    15121 ttatttactt ctgctgcacg aaattgcggt aagccgccac gacctgcaga aaatcctcaa
    15181 cgccgcatag cgacaggctt tcttcgtcgt agtagttcat cccctcttcc atttcatcgc
    15241 cagcgaattc cagttgattg gcgcgaacca tcacctcttc agcgtccatc cacagggtgt
    15301 attcatgccc tgcccgttgc caggaccgtt cgctaccttt cagtgcgtgc gctgcttgtt
    15361 ccacttcatc aagcaaggcc aggttttctt tcacctcttc attaaaccag tgcccgacca
    15421 cttcatgccc catggacata cgcaccttta ccactccggt aatatcgcgc agaaattcgt
    15481 aatccatagt gtgttcctct gctcccggca atgcgccgaa atcgcatcgc gccattgatg
    15541 taattatcgc agccttaccg gagaaaaaca gcggaacggc gatagtttag aaataaaaaa
    15601 tgcccgggaa tctcattcac cgggcattgt gtcgtttatg cgcagcgcgt gcgcttactt
    15661 attacaccgc agtctggaaa atcaccccat cggctttttc ggtgtactga gaaagctggt
    15721 tgaagttcag ataacgataa gtatcaacgg cagttttatc tacttgcgca acgtaggtct
    15781 ggtactcttc cggcgtcggc agtttgccaa tcagcgccgc aacagccgcc agttctgcag
    15841 aagccaggaa gacattcgcg ccagtaccca aacggttcgg gaagttacgg gtagaggtgg
    15901 aaaccaccgt cgcaccgtct gctacacgcg cctggttacc catacacagg gaacagcccg
    15961 ggatctcgat acgagccccg ctcttaccga agacgctgta gtagccttct tccgtcagtt
    16021 gcgcggcgtc catacgggtt ggcggtgcca cccacaggcg ggtcggcaac tgacctttgt
    16081 gcgcatccag cagtttacca gcggcacgga agtgaccgat gttagtcatg caagaaccga
    16141 taaatacttc gtcgatcttc tcgccctgta ccgcagacag cggacgtgcg tcatccggat
    16201 cgttcggagc acacaggatt ggctctttaa tctccgccag atcgatgtcg atcactgccg
    16261 cgtattccgc atctgcatcg gcttccagca gctcaggatt cgccagccat ttttccatgc
    16321 cctgaatacg gcgttccagg gtacgacgat cgccgtaacc ttccgcgatc atccacttca
    16381 gcaggacgat gttggagctc aggtattcga tgatcggttc tttgttcagc ttgatagtac
    16441 aaccagcggc agaacgctcg gcggacgcat cggttagctc aaaggcctgc tcaactttca
    16501 gatccggcag accttcaatt tccagaatgc gaccagagaa gatgtttttc ttgcctttct
    16561 tctcaacggt cagcagacct tgtttgatcg catacagcgg aatggcgtgt accagatcgc
    16621 gcagggtgat gcccggctgc attttgcctt tgaagcgcac cagaacggat tccggcatat
    16681 ccagcggcat tacgccagtt gcggcagcaa acgccaccag accagaaccc gccgggaaag
    16741 agataccgat cgggaaacgg gtatgggagt caccaccggt accgacggta tccggcagca
    16801 gcatacggtt cagccaggag tgaatgacgc cgtcacccgg acgcagcgac acaccgccac
    16861 ggttcataat gaagtccggc agcgtgtggt gcgtgttcac gtcaactggc ttcggatacg
    16921 ccgcggtgtg gcagaaagac tgcatcacca ggtcagccga gaagcccagg cacgccaggt
    16981 ctttcagttc atcacgggtc atcgggccgg tggtgtcctg agagcctaca gaagtcattt
    17041 tcggttcgca gtacgcgccc ggacgaatgc ctttcacgcc acaggcacgg cctaccattt
    17101 tctgcgccag cgagaagccg cgatcgctct cagcgacatc tttcgcctga cggaacacat
    17161 cactgtgcgg cagaccaagt gcttcacgcg ctttggtggt caggccacgc ccgataatca
    17221 gcggaatacg gccgccagca cgcacttcat caatcagcac gtcggttttc agttcgaagg
    17281 tcgccagcag ttcgcctgtt tcgtggttac gcacttcacc tttgtacggg taaacgtcaa
    17341 tcacgtcgcc catgttcagg ttagagacgt tgacttcgat tggcagtgca cccgcgtctt
    17401 ccatcgtgtt aaagaagatg ggtgcaattt taccgccgag gcacaaacca ccgccgcgtt
    17461 tgttcggcac atgtggaata tcatcgccca taaaccacag aacggagttc gtggcggatt
    17521 tacgcgatga acccgtaccc acaacgtcac cgacgtacgc cagcgggaaa cctttctgtt
    17581 gcagagcttc aatctgtttg atcgggccaa caacacctgg ctggtctggc tcaatacctt
    17641 cacgggcgtt tttcagcatc gccagcgcgt gcagtgggat atccgggcgt gaccacgcat
    17701 ccggtgccgg agaaaggtca tcggtgttgg tttcgccagt gactttaaag acggtaacgg
    17761 tcagtttttc agccagcgcc gggcgattca ggaaccattc ggcatccgcc caggactgca
    17821 ttacctgctt cgcatattcg ttgcctgctt tcgctttctc ttctacgtca tagaagttat
    17881 cgaacatcag cagcgtgtga gaaagtgctt tggcagcaat aggtgccagt ttggcatcat
    17941 ccagcgcgtc gatcagcgga tgaatgttgt aaccaccctg catggtgccc agcagttcga
    18001 tagctttttc cggagtcagc agaggggatt tagcttcgcc tttagcgata gcagccagga
    18061 agcctgcttt tacataggcg gcttcatcga cgcctggggg aacacggttg gttaacagat
    18121 ctaacaggaa ttcttcttcg cccgcgggcg ggtttttcag cagctctaca agcgcggcca
    18181 tttggtttgc atccaggggt ttgggcgcaa tcccctcagc ggcacgctca gctacgtgct
    18241 tacggtattc ttctagcacg acggttctcc tcgctctcat tgtcatagtg cggcaggcga
    18301 ttctcttcac gctcctgtga gacagcagtt tgtagggtaa atgcccagta ccgcaacggg
    18361 cagaatagca ggattttgac gacgtgttaa tctgtttaca aaaaagcaac ataaaaaagt
    18421 ggctgaatcg ttaaggatgg tctagagatt gtttccctcc gtaaaaactt cgttaatttg
    18481 catggtgata atcacctttc tcaacgaatc ccaagattag tcaaaattta aactaccgcc
    18541 tctttatact cggattcaca gcacctgcgg gtggcagttc gccgaccatt gcgatttcct
    18601 tgagatccga attatgaaaa tgactttgcc gtttaaaccc catgtgctgg cgctaatttg
    18661 cagtgccggg ctttgtgctg cctctgccgg gctatatata aaaagccgca cagtggaagc
    18721 gcctgtagaa ccgcaatcga cacaactggc tgtgtctgac gctgccgctg ttacgcttcc
    18781 tgcaacggtt tccgcacctc ccgtaatacc cgcagtcgtc aaatccgcat tcagcactgc
    18841 acaaatagat caatgggtcg cacccgtcgc gctgtatccc gacgccctac tttcgcaggt
    18901 gctgatggca tcaacctatc cgacaaacgt tgctcaagca gtgcaatggt cgcacgataa
    18961 tccacttaaa caaggcgatg ctgctattca ggcggtatct gaccagccgt gggacgccag
    19021 cgttaaatca ctggtggcct ttccacaatt gatggcattg atgggcgaaa acccgcaatg
    19081 ggtgcaaaac ctgggcgatg cttttctggc gcagccgcag gacgtgatgg actcggtaca
    19141 gcgattgcgg caactggcgc aacaaaccgg ctcgctgaag tcatcaaccg aacagaaagt
    19201 tattaccaca acgaagaaag ctgtaccggt aaaacagaca gtcacggcac ccgtcatacc
    19261 atccaatacc gttttaactg ccaaccccgt cattacagag ccggcaacaa ccgtcatttc
    19321 cattgagccc gccaatcctg atgtggtcta tattcccaac tacaacccaa ccgtggttta
    19381 cgggaactgg gccaatactg cgtatccgcc ggtttatctg ccaccaccag ccggagaacc
    19441 gtttgttgac agctttgtgc gcggattcgg ctatagcatg ggcgttgcta ccacgtacgc
    19501 actattcagc agcatcgact gagatgacga cgatcatgac catcatcatc atgacgatga
    19561 tgattatcat caccacgatg gcggtcatcg tgacagtaat ggctggcaac acaacggcga
    19621 caacatcaat atcgacgtca acaatttcaa ccgtatcacc ggtgagcatc ttactgataa
    19681 gaatatggca tggcggcaca atccaaacta ccgtaatggt gtgccctatc atgatcagga
    19741 tatggcaaag cggtttcatc aaaccgatgt caacggcgga atgagtgcca cgcagctacc
    19801 tgctccaaca cgcgacagcc agcgtcaggc ggcagcaagt cagtttcagc aacgaacaca
    19861 cgccgccccc gtcattacac gagataccca acgtcaggca gcggcacagc ggtttaatga
    19921 agctgaacac tatgggagct atgacgactt ccgcgacttc agccgtcgcc aaccactgac
    19981 ccagcagcaa aaggacgccg ctcgtcagcg ttatcagtcg gcctctcctg agcagcgcca
    20041 ggcagtccgc gagaaaatgc agactaaccc gcagatccag cagcgaaggg aggcggcgcg
    20101 ccagcgtatt cagtccgcct cgcctgagca gcgccaggcg gttcgcgaga aaatgcagac
    20161 taacccgcag aaccagcagc gaagagaggc agcgcgtgag cgtattcagt ctgcaactcc
    20221 cgaacagcgc caggtgttta aggaaaaagt acagcagcgc ccactgaacc aacagcaacg
    20281 tgataacgcc cgccagcgta tacaatcggc atcacctgaa caacgtcagg tttttcggga
    20341 gaaagttcag gagagccgcc cacaacgtct aaacgacagt aaccatactg tcaggctgaa
    20401 taacgagcaa cggtcagcag tacgcgaacg tctctctgaa cgtggagcaa gaagactgga
    20461 aaggtaaatt gcagacgtaa aaaaagcggc gtggttagcc gcttttttaa ttgccggatg
    20521 ttccggcaaa cgaaaaatta cttcttcttc gctttcgggt tcggcaggtc ggtaatgcta
    20581 ccttcgaaca cttctgccgc caggcccaca gactcgtgca gagtcgggtg cgcgtggatg
    20641 gtcagtgcga tgtcttcagc atcacaaccc atttcgattg ccaggccgat ttcacccagc
    20701 agctcgccgc cgttggtacc aacaatcgca ccaccgatca cacggtgaga ttctttgtcg
    20761 aaaatcagct tggtcatacc gtctgcgcag tcagaagcga tagcacgacc agaagcagcc
    20821 cacgggaagg tggcggtttc atagctgatg cctttctctt tcgcttcttt ctcagtcaga
    20881 cctacccatg caacttctgg ttcggtatag gcgatggacg ggataacttt cggatcgaag
    20941 tagtgtttct taccggcgat aacttcagcg gcaacgtgac cttcgtgaac acctttgtgt
    21001 gccagcatcg gctgaccgac gatatcgccg atagcaaaga tgtgcggtac gttggtacgc
    21061 agctgtttgt caacgcggat gaaaccacgg tcgtcaactt ccacgccagc tttgcctgcg
    21121 tcgaggtttt taccgttcgg cacacgacca atcgctacca gcacggcgtc gtaacgctgc
    21181 ggttcagctg gtgctttttt gccttccatc gtcacataaa taccgtcttc tttcgcttca
    21241 acggcggtaa ctttggtttc cagcatcagg ttgaatttct tgctgatacg cttggtgaag
    21301 actttaacga tgtctttgtc agctgccggg ataacctggt cgaacatttc aaccacgtca
    21361 atctgtgaac ccagcgcgtg gtaaacggtg cccatttcca gaccgatgat accgccaccc
    21421 attaccagca ggcgttctgg tacttctttc agttccagcg cgtcagtgga gtcccagata
    21481 cgcggatctt catgcggaat aaacggcagt tggatcgggc gagaacccgc tgcaatgatc
    21541 gcattgtcga agttgatcac ggttttgccg ttctcacctt caacttccag ggtgttagcc
    21601 ccggtgaatt tacccagacc gttgaccact ttgactttgc ggcctttcgc cataccagcc
    21661 agaccaccgg tcagctgatt gatcactttc tctttccagg tacgaatctt gtcaatatcg
    21721 gttttcggtt cgccgaagac gataccgtgt tcagccagcg ctttggcttc ttcgataact
    21781 tttgctacgt gcagcagtgc tttagaaggg atacagccga cgttcaggca aacaccgcca
    21841 agggtgttgt aacgttctac gattacggtt tccagaccta aatcagcgca acggaaggca
    21901 gcggagtaac ctgcggggcc tgccccaagt accacgacct gagttttgat ttcagtactc
    21961 atcatgacct ctatatattt atctccggcg gtcatacccg tcgtctttca ggccgcagcg
    22021 gcgttagctt cacgccctca ccccagtcac ttacttatgt aagctcctgg ggattctggc
    22081 gcttgctacc ttgctgcacc cagaaatcca tagggtatgt gaagtaatac cctaaccacc
    22141 accgggtcgt tctatccgtc ggtattttac aaaattgtta acaattttta aacaacaaac
    22201 ggcaaccgat ttgtctattc gctaataacc tcaatacatt catgagatta ccagaaaaaa
    22261 gccggccgtt gggccggctc ttttacttac atcaccagac ggcgaatgtc agacagcgtg
    22321 ttgttaatga tggtaatgaa acgggcacca tcagcaccgt cgatcacgcg gtggtcgaag
    22381 gagagagaaa tcggcagcat cagacgcggc acgaactctt taccattcca caccggctcc
    22441 atcgcggact tggaaacgcc gaggatagcc acttccggcg cgttcacaat cggcgcgaag
    22501 tgggtagtac ccaggccgcc gatgctggag atggtgaagc aaccgccctg catttcgccc
    22561 gcagtcagct taccgtcacg cgctttctta gaaatagtca tcagctcgcg agacagctcg
    22621 atgatgcctt tcttgttgac gtctttgaat accggaacaa ccagaccgtt cggggtatcc
    22681 accgccacac cgatgttgat gtatttcttc agggtcagac gctgaccgtc ttccgacagc
    22741 gaactattga agcgaggcat ctgctcaaga gctgcagcaa cggctttcat gatgaagaca
    22801 accggggtga tcttcacatc cagcttacgt ttcgccgctt cttcgttctg ctgtttacgg
    22861 aacgcttcca gctcggtgat atcggttttg tcgaagtggg taacatgcgg gatcatcacc
    22921 cagttacggc tcaggttagc accagaaatt ttctggatac ggcccagttc cacttcttcg
    22981 atttcgccaa acttgctgaa gtccaccttc ggccacggca gcatgccagg gataccaccg
    23041 ccagtcgccg ccggagctgc ttctgcacgt ttgatagctt ctttcacgta agcctgaacg
    23101 tcttcgcgca ggatacgacc tttacggcca gtgcccttca ctttcgccag gttaacgcca
    23161 aactcgcgtg ccagacggcg gatcagcggg gtcgcgtgaa cgtaagcgtc gttttcagca
    23221 aattcagatt tgccttccgc tttcgcagcc ggagcttcag cttttgctgc cggagccggc
    23281 gctgcgcctt caacttcgaa gatcataatc agcgaaccag ttttcacttt atcgccaacg
    23341 ttgactttca gttccttcac gacgcctgca aacggagccg gaacttccat agaagcttta
    23401 tcgccttcta cggtaatcag tgactgttca gcggcaactt tgtcgcccac tttcaccatc
    23461 acttcggtca cttcaacttc gtcaccgccg atatccggaa cgttaacttc tttcacgcca
    23521 gctgctggtg caggggtcgc tgccggagtc gcttcctgtt tagccgccgg agctgccgcg
    23581 cctgcttcac ccgcgacttc gaagaccata atcagcgagc cggtagacac tttgtcaccc
    23641 acgttcactt tgatctcttt cacggtacct gcaaacggag ccggaacttc catagaagcc
    23701 ttgtcgcctt ctacggtgat cagcgactgt tcagcttcaa ctttatcgcc aactttcacc
    23761 aggatttcgg tcacttcaac ttcgtcgctg ccgatatccg gaacgttaac gtcttttgcc
    23821 gccgcagccg cgggtgctgc tgccggagct gcttctttct tctcttctgc ctgagcaggt
    23881 gcagcgtctg ctgcaccgtc ggcggaatcg aaaatcataa tcagtgcgcc ggtctgggtt
    23941 ttatcgccaa cagagacttt gatctcttta acgatacccg cctgcggaga tggaacttcc
    24001 atagaggctt tgtcgccttc tacggtgatc agcgactgtt cggcttcaac tttgtcgccc
    24061 actttgacca ggatctcggt gatttcaact tcatcagccc cgatgtccgg tactttgatt
    24121 tcgatagcca ttattctttt acctcttacg ccagacgcgg gttaacttta tctgcatcga
    24181 tgttgaattt ggcgattgcg tcagcaacca ctttcttatc gatttcgcca cgtttagcca
    24241 gttcgcccag cgccgcaacc acgacataag aagcatcaac ttcgaagtgg tgacgcaggt
    24301 tctcacggct gtcggaacga ccgaagccat cagtacccag tacgcgatag tcgtcagccg
    24361 gtacgtaagt acggacctgc tcagcgaaca gtttcatata gtcggtagat gccactgccg
    24421 gcgcgtcgtt catcacctga gcgatatacg gaacgcgcgg agtttccagc ggatgcagca
    24481 tgttccagcg ttcacaatcc tgaccatcac gcgccagctc ggtgaaggag gtcacgctat
    24541 aaacgtcaga acctacgccg taatctttcg ccaggatctc tgccgcttca cggacgtgac
    24601 gcaggatgga accggagccc agcagctgaa ctttaccttt gctaccttca atggtttcga
    24661 gtttgtagat acctttacgg ataccttcct cagcaccttc cggcattgcc ggcatgtggt
    24721 agttttcgtt cagcgtagtg atgtagtagt aaacgttctc ttgtttttca ccgtacatac
    24781 gctccagacc gtcatgcata atgacagcaa cttcgtaagc gtaagccggg tcgtaagaga
    24841 tacagttcgg gatagtcagc gactgaatgt ggctgtgacc atcttcgtgc tgcagacctt
    24901 caccgttcag ggtggtacga ccggaagtac cgccgatcag gaagccacgc gcttgctggt
    24961 ctccagccgc ccagcacaga tcgccgatac gctggaagcc gaacatcgag taatagatgt
    25021 agaatgggat catcggcaga ttgttcgtgc tgtaagaggt cgccgctgcc agccaggaac
    25081 accctgcgcc cagctcgttg atcccttcct gcagaatctg acctttctcg tcttctttat
    25141 agtaagcaac ctgctcgcgg tcctgcgggg tgtactgctg accgttcggg ctgtaaatac
    25201 caatctgacg gaacagacct tccataccga aagtacgcgc ttcgtcggcg atgatcggta
    25261 ccagacgatc tttgatcgac ttgttcttca gcatcacgtt cagagcacga acgaaagcga
    25321 tagtggtaga gagctctttg ctctgctctt ccagcagcgc gccgaagtct tgcaggctcg
    25381 gcagctcaag cttctcggtg aagttcggct gacggcttgg cagataaccg tgcagtttct
    25441 gacgctgagc gtgcagatag gtatgctctt cagaaccttc cgggaaggtg atgtacggca
    25501 gtttttcgat atctgcatca gacaccggca cattgaaacg gtcgcggatg tgacgcacgc
    25561 cgtccatgtt cattttctta acctggtgcg cgatgttttt accttcagcc gcgtcgccca
    25621 tgccgtaacc tttaatggta tgagcaagga ttactgtcgc tttgcctttg gtttcctgcg
    25681 ctttcttgaa tgcagcgtag attttcttcg gatcgtgacc accacggttc agtgcccaga
    25741 tctgctcgtc agtccagtct gcaaccagtg ctgcggtttc aggatattta ccgaagaagt
    25801 gttcacgaac gtacgcacca tctttcgatt tgaaggtctg gtagtcgccg tcaacggttt
    25861 cgttcatcag ctggatcagt ttaccgctgg tatctttacg cagcagttca tcccaacggc
    25921 taccccacat cactttgatc acgttccagc cagcaccttc gaagatgcct tccagttcgt
    25981 tgatgatctt gccgttaccg gtgaccgggc cgtcaagacg ctgcaggtta cagttgataa
    26041 cgaaaaccag gttatccagt ttttcacggg tagcgatggt gatcgcacct ttggattccg
    26101 gttcgtccat ttcaccgtcg ccgaggaacg cgtaaacggt ctgtttagag gtatctttca
    26161 ggccacggtg ttccagatat ttcaggaatt tagcctggta aatagcacca atcggaccca
    26221 gacccataga tacggtcggg aactgccaga attccggcat cagtttcggg tgcggatagg
    26281 aagagaggcc attgccgtga acttcctgac ggaagttatc cagctgctcc tgagtcagac
    26341 gaccttccag gaaagcacgg gcgtaaacgc ccggggagat gtggccctgg aagtaaacca
    26401 ggtcgccgcc atcctgctcg ttgcgtgcac ggaagaagtg gttaaagcac acatcataaa
    26461 tggttgcgga agactggaag gacgccatgt ggccgcccag ttcgaggtct tttttcgacg
    26521 cacgcagcac cgtcatgatg gcgttccagc ggatagctga acgaatacgg cgttccagtt
    26581 ccagattacc cggatactcc ggttgttctt caacggggat ggtgttgatg tagttgctga
    26641 tacctgtgcc tgcggctacg tttacaccgc ctttgcgggc ttcagcaagc agttggtcga
    26701 tcagatactg agcacgctca acaccttctt cacggatgac cgattcgatc gcctggagcc
    26761 agtcgcgagt ttcgatcgga tccacgtcat ttgggaaacg ttctgacatg ggttattcct
    26821 tatctatcta ataacgttga gttttctgga acctgtccca ttgaactctc gccagaaagc
    26881 tcaataagac aggttctacg tttagttacc gcgcttttat atgcgcttga tttacaacat
    26941 cttctggata atttttacca gaaaaatcac taattctttc gttgctccag acgacgcaga
    27001 gaacgctcac ggcggctctc ttcacggctt ctgtcgagca aaatttcttc gataaaggcc
    27061 agatggcgat gcgatgcttc gcgcgcttct tccggcttac cggccataat cgcttcaaat
    27121 atgcgggtgc ggtgactact caccagcggc agcatctcgc gacgcgaata gagcaattcg
    27181 aagttctggc ggacattctg ggccaacatc ggctccatac accttagcag atgaagcaga
    27241 accacattgt gggccgcttc ggtgacggca atctgatact ggagtacggc gtttgattcc
    27301 gcgtccagat cgccagactg ctgcgccagc tctatggcgt ggtggagttc acggatgcgt
    27361 tccttgtctt catcggtact acgcagcgcg gcgtaataag cggcgatacc ttccagggcg
    27421 tgtcgtgttt cgagcaagtc atactgtgat tcaggatggt cggagagcag ctccaccagc
    27481 ggatcgctga agctttgcca taggctgctc tggacaaaag tgccgccacc ctggcgacga
    27541 agcaacaagc ccttcgcttc gagacgttga atcgcctcac gcaaggaggg acgggagacg
    27601 tcgaactgtt ttgccagttc gcgttccggt gggagttttt cgcccgggcg gagagtgcct
    27661 tcgaggatca aaaactccag ttgctgctca atcacatcgg agagttttgg ttggcggatt
    27721 ttgctgtagg ccatgagttc ctgtcttaag ccacttgccg aagtcaattg gtcttaccaa
    27781 tttcatgtct gtgacgctaa agtaacaaag tattcacctt atgtccatac aggttttgat
    27841 tgaaatcatg aaactgtgca cattttaaca acttgacata tataacgttt caaagttgta
    27901 actatgcaca aatgtagact ttacgtagga aagggatttt ttaacgatta ttaaactata
    27961 aaaatccgaa ttgaaccgat tcacttacca attttttgat ttttaagtca ataaaaacga
    28021 atttaaattc actctgcata ttgagagggg ttgaggctga gctttacaaa cggtttcttt
    28081 ttaagcaact catcttcaac catgcataaa gcgggtgcat tcgctgccgc ataccattat
    28141 tcttgatctg acggaagtct ttttgtaaca attcaaactt ccttgatgta aataaatcaa
    28201 tacaacaaac ggaattgcaa acttacacac gcatcactgc gtagatcaaa aaaacaatca
    28261 ccgcacgagg tttcatgatg gaaggtcaac agcacggcga gcagctaaag cgcggcctta
    28321 aaaaccgcca tattcagctt atcgcgctgg gtggcgcgat agggacaggg ctattcctag
    28381 gtagcgcctc cgtaatacag tccgcagggc cagggattat cctgggttac gccattgctg
    28441 gttttatcgc ctttctgatc atgcgtcagc tgggtgaaat ggtggtcgaa gaacctgtcg
    28501 caggctcctt tagccacttt gcttataaat actggggcag ttttgccggt ttcgcctctg
    28561 gctggaacta ctgggtactg tacgttttag ttgccatggc agagctgact gccgtgggta
    28621 aatacattca gttctggtat ccggaaatcc caacctgggt ttctgccgcc gtattctttg
    28681 tggtgattaa cgccatcaac ctgaccaacg ttaaagtgtt tggcgagatg gagttctggt
    28741 ttgccattat caaagttatt gcggtagtag cgatgatcat cttcggcggc tggctgctgt
    28801 tcagtggcaa cggcggcccg caggcgagcg ttagcaacct gtgggatcaa ggtggtttcc
    28861 tgccgcacgg cttcaccgga ctggtgatga tgatggcgat tatcatgttc tcgttcggtg
    28921 gtctggaact ggtggggatc accgcagcag aagctgataa cccggagcaa agtatcccga
    28981 aagcaactaa ccaggttatc taccgcatcc tgattttcta tattggttcg ttagccgttc
    29041 tgctctcact gatgccgtgg acccgcgtta ccgccgatac cagtccgttt gtgctgatct
    29101 tccacgagtt aggcgatacc tttgtggcga atgcgctgaa catcgtggta ctgactgcgg
    29161 cgctctccgt gtacaacagc tgcgtatatt gcaacagccg tatgctgttt ggtctggcac
    29221 aacaggggaa cgcgccaaaa gcgctggcgt ctgtcgataa acgcggcgta ccggttaaca
    29281 ccattctggt gtctgcgctg gttacagcat tgtgcgtatt gattaactat cttgctccgg
    29341 aatccgcatt tggcctgtta atggcactgg tggtatccgc actggtgatc aactggacga
    29401 tgatcagtct ggcgcatatg aagttccgtc gcgccaagca ggaacaaggc gtggtaactc
    29461 gcttccctgc tctgctttat ccgctgggta actggatctg cctgctgttt atggcggcgg
    29521 tactggtgat tatgctgatg accccaggaa tggcgatttc ggtatacctg atcccggtat
    29581 ggctgatcgt gttaggtatc ggctatctgt ttaaagagaa aaccgccaaa gccgtaaaag
    29641 cgcattaatc tctctacgcc ctcacccgta cagggtgagg gcaataatct ctacaccaac
    29701 gccccgtaaa tcgtcagtag cgcaatcacc accacgacca cgaacgaggt tttcttcgcc
    29761 attgaaaccg ctgccttcgg cgtctccacc ttatcgacat gcggttcacg cgccagagag
    29821 aactgcgcca gacgcgttaa tacctgatat tgcgaagtat ggaaatcacc cagcgaagca
    29881 aaccaggccg gtaacgcttt ctcaccatga ccgatcaagg catataccac acccgcaaga
    29941 cgaaccggca cccaatccag tacatgaagc actgcatcaa tgccggactg taaacgatga
    30001 tgcggcgtct ggtatcgcgc cagccagtat tgccatgcac gcaagaacgc ataccccatc
    30061 agcgtaacgg gtccccaggt tccccccaca atcagccaga acagcggtgc aagataaaaa
    30121 cgaaagttaa tccacagcaa tgcattttgc agctcacgca aatactcacg ttcgtcgcag
    30181 cctgccggga cgccgtgaat catggtgagt tcgccagcca tcgtggcacg ggcatggcta
    30241 tcattacgtg aagcagctgt cagataagca tgataatgaa gacgaacttt acctgcgcca
    30301 atacacagca aaccaatcag cagccacacc agcagcgtgg gaacgttgaa caatactccc
    30361 tgcaatgcgc gtaacagtaa aaaagtcaca cccatcgcaa taatggtcat gcctaacgtg
    30421 cgcccgagag aaaaatgttt cacccgccga aagaacgctt caagacgatg atcaagctgc
    30481 cagtgctcgc ccaacttaaa caggcgctcg aaaattaaca ccagtaaggt tgtaaatagc
    30541 gtcatgttgt ctccttgctg accagcgcac gaaaccgtgc ccaatcaaat gcaggaccgg
    30601 gatcggtttt ccgatccggc gcaatatcac aatggcccgt catgttatta gcgatatcgg
    30661 gatagcgatc tatcagtgcc cgcgtaaccg ccgcaagctg ttgatactgc gcatcggtat
    30721 acgccagagt atcggtgcct tcaagctcaa tcccaataga aaaatcattg cagcgttcac
    30781 gcccctgata ctgagagact cccgcatgcc aggcacgttt atcgaaagga acatactgga
    30841 ctatttcacc atcacggcga atcaaacagt gagcggagac gcgcaaatgg gcgatctcag
    30901 caaagaaagg atgtgcctgc ggatcaatag ttccagtgaa taatgcgtcg atccacggac
    30961 cgccaaactc gcctggcggc aggctaatat tgtgcaccac cagcagggtg ggtgtttcgt
    31021 catccaggcg gcaatcgtaa tgtggtgagg gaacgcggcg cgcgccaacc agccacccct
    31081 gttctaacaa catgcaggat ctccttatat gtggtgctaa tacccggttc agagtagcat
    31141 gtttctacct tatgattcgt tagctatctg gagttttaac atgccgcctc gccgctacaa
    31201 ccctgacacc cgacgtgacg agctgctgga acgcattaat ctcgatatcc ccggcgcggt
    31261 ggcccaggcg ctgcgggaag atttaggcgg aacagtcgat gccaacaatg acattacggc
    31321 aaaactttta ccggaaaatt ctcgctctca tgccacggtg atcacccgcg agaatggcgt
    31381 attttgtggc aaacgctggg ttgaagaggt gtttattcaa ctggcaggcg acgatgtcac
    31441 cataatctgg catgtggatg acggcgatgt catcaacgcc aatcaaccct tgttcgaact
    31501 tgaaggccca tcccgcgtgc tgttaacggg cgaacgcact gcgcttaatt ttgtgcaaac
    31561 cctttcagga gttgccagta aggtacgcca ctatgtcgaa ttactggaag gcaccaacac
    31621 gcagttgttg gatacgcgca aaaccttacc tggcctgcgt tcagctctga aatacgcggt
    31681 actttgcggc ggcggagcga atcaccgtct ggggctttct gatgccttcc tgatcaaaga
    31741 aaaccatatt attgcctccg gctcagtgcg ccaggcggtc gaaaaagcgt cctggctgca
    31801 tccggatgcg ccagtagaag tcgaagtaga gaatctggaa gaacttgatg aggccctgaa
    31861 agccggagcc gatatcatca tgctggataa cttcgaaaca gaacagatgc gcgaagccgt
    31921 caaacgcacc aacggcaagg cgctactgga agtgtctggc aacgtcactg acaaaacact
    31981 gcgcgaattt gccgaaacgg gcgtggactt tatctccgtc ggtgcgctaa ctaaacacgt
    32041 acaagcactc gacctttcaa tgcgttttcg ctaattgctg acagatttat ccgctccatc
    32101 gcgggcggat aatctcccct tccccaactt tcttcgtaac gcctcgcaaa tttcttgtct
    32161 ttcgctgcaa cgaaataaaa aacctcggaa tctgctttcc cgtttcgttt tttgcccctc
    32221 gcctgtgctg atggcgtttg ccaaagtagc accaaccaaa tcaaggagcg aaacagatgg
    32281 acaagcaacg cggttttaca cttatcgaac tgatggtggt tattggcatc attgccattt
    32341 taagctccat tggtattccc gcttatcaaa actacctgcg caaagccgca ctcaccgaca
    32401 tgctacaaac ctttgtgcct taccgtaccg ccgtagagtt gtgcgcgctg gaacatggtg
    32461 gattagatac ctgcgacggt ggcagcaatg gcattccctc gcctaccacc acccgctatg
    32521 tttcagccat gagtgtggca aagggcgtgg tgtcgctgac cgggcaagaa agtctcaatg
    32581 ggctaagcgt cgtcatgacg ccaggttggg ataacgcaaa cggcgtcacc ggctgggcgc
    32641 gcaactgcaa tattcaaagt gacagcgcat tgcagcaagc ctgcgaagat gtcttccgct
    32701 ttgatgacgc caactaagga gcggcaatga atattccaca gctcacggcc ctgtgcctgc
    32761 gttatcaggg agtcttgctg gatgccagcg aagaagtggt tcatgttgcg gtggtcgatg
    32821 ccccctcaca tgagttgctg gacgcattgc atttcgctac caccaaacgt attgagatca
    32881 cctgctggac gcgccaacaa atggaaggtc acgccagtcg cacacaacag acattacccg
    32941 tagctgttca ggagaagcat cagcccaaag cagaattgct gactcgaacg ttacaatctg
    33001 cgctggaaca acgcgcgtct gatattcata tcgaaccagc ggacaatgcc taccgcatcc
    33061 gcttgcgtat cgacggcgta ttgcatcctt taccggatgt ttcaccggat gccggagtcg
    33121 cattaacagc cagattaaaa gtgctgggaa acctggatat tgcggaacat cgcctgccgc
    33181 aggacgggca attcactgtc gaactggcag gaaacaccgt ctcatttcgt attgcgacct
    33241 taccatgtcg gggtggtgaa aaggtggtat taaggttgtt acagcaggtg agccaggcac
    33301 tggatgttaa cacgctggga atgcagccgt tacaactggc ggactttgct catgccttgc
    33361 aacaaccaca gggactggtg ctggtaactg gccctacagg cagtggcaaa acggtcacct
    33421 ggtgttgtcc acgctacaca ccaattccac cactgaaacg ctggtacgtt tacagcaaat
    33481 gggggtcgcc cgctggatgc tatcatcggc gcttacgctg gtaatagccc agcgtctggt
    33541 acgtaaactt tgcccacatt gtcgccgaca gcaaggggag cccatccata ttccagacac
    33601 tgtatggccg tcgccgctgc cccactggca ggcacccggt tgtgtacatt gctaccacgg
    33661 tttttatggt cgtaccgcct tgtttgaagt cctgcccatt acaccagcca tacgccagct
    33721 tatttccgct aatactgaag ttgaatcgct ggaaacgcac gcccgacagg cgggtatgcg
    33781 tacgcttttt gaaaacggct gcctggccgt ggagcaaggc ttaaccacct ttgaagagtt
    33841 aatccgcgta ctggggatgc cgcatggcaa gtaagcaact ctggcgctgg catggcataa
    33901 ccggcgacgg caatgcgcaa gatgggatgc tatgggcaga gagccgtact ttactgctta
    33961 tggcactaca gcaacagatg gttaccccac taagcctgaa gcgaatcgcc atcaattctg
    34021 cgcagtggcg aggagataaa agcgcggaag tcattcatca actggcgacg ctacttaaag
    34081 ccgggttaac gctttctgaa gggctggctc tgctggcgga acagcatccc agtaagcaat
    34141 ggcaagcgtt gctgcaatcg ctggcgcacg atctcgaaca gggcattgct ttttccaatg
    34201 ccttattacc ctggtcagag gcatttccgc cgctctatca ggcgatgatc cgcacgggtg
    34261 aactgaccgg taagctggat gaatgctgct ttgaactggc gcgtcagcaa aaatcccagc
    34321 gtcagttgac cgacaaagtg aaatcagcgt tgcgttatcc catcatcatt ttagcgatgg
    34381 caatcatggt ggttgtggca atgctgcatt ttgttctgcc ggagtttgcc gctatctata
    34441 agaccttcaa caccccacta ccggcactaa cgcaggggat catgacgctg gcagacttta
    34501 gtggcgaatg gggctggctg ctggtgttgt tcggctttct gctggcgata gccaataagt
    34561 tgctgatgcg ccgcccgacc tggcttatcg cgcggcagaa attgctgtta cgcatcccga
    34621 ttatgggttc actgatgagg ggacaaaaac tcacgcagat ctttacgatt ctggcgctga
    34681 cacaaagtgc aggcattact tttttacagg gcgtagagag cgtcagagaa acaatgcgct
    34741 gcccgtactg ggtgcaactt ctgacacaaa tccagcacga tatcagtaac ggtcatccca
    34801 tctggctggc gctaaaaaat gccggtgagt ttagcccgct ctgtttgcaa ttagtgagaa
    34861 caggagaggc atccggctcg ctggacctca tgttagacaa cctcgcccat catcatcggg
    34921 ataacacaat ggcgctggcg gataacctcg cagccttact ggaaccggcg ttgctgatca
    34981 taacgggagg aattatcggt acgctggtgg tggcaatgta tctgccaatt ttccatttag
    35041 gcgatgcgat gagtgggatg ggataacgct ggcgtgttgc tccacgccag cgttgggaga
    35101 ttacaggttg ttgaagatgc ggttttcttg ttccagcaca cgaataaacg tggtgcgctt
    35161 ggtcagctct ttcaggcgtg aagccccaac gtatgtacaa gctgaacgca ggccgcccaa
    35221 aatatcacgc gcggtatttt caacctggcc tcgcagcggc agcttaacgg ttttaccttc
    35281 tgctgcgcgg tattccgcaa cgccgccaac gtgacgtttc atcgcagact cggagctcat
    35341 gccgtagaac agcataaatt tctcgccgtt ctcctcaacg atgcgaccgc cgctctcttc
    35401 gtggcccgcc agcatgccgc caagcatgac gaaatcggca ccgccgccaa aggctttcgc
    35461 cacatcgccc ggcgtggtac agccaccgtc gctgacgatc attccgccca gaccgtgcgc
    35521 cgcatcggca cattcgatta ccgcagaaag ctgcggataa ccgacgcctg ttttgacgcg
    35581 agttgtacaa acagaacctg ggccaatgcc aactttaacg atatcagcac cggagaggat
    35641 aagctcctca cacatttcac cagtcactac gttgccagca caaatggttt tggtcggcca
    35701 cgcttcacgc gctttcgcaa cgaactgcac gaagtgttcg gaataaccat tcgccacatc
    35761 aatacaaacg aagtttaatg ccgggttcag gtcgagaatc tgtttagttt tttcgaaatc
    35821 cgcatcagac gtgccggtag aaaccatcac atgtttcagc acatcagcgg aagaattgtt
    35881 gataaacgct tgccactctt cgacagaaaa gtgtttatgc acagcagtca gaatatcaaa
    35941 agaagccagc gcagaggcca tagaaaatgt gcctacggtg tccatatttg cggcgataat
    36001 cggcacgccg gaccagctct gacctgaatg tttgaaggtg aattgacgtt ccagttcaac
    36061 atcggaacgg cttttaagag tggagcgttt agggcggatg agaacgtctt taaaacctaa
    36121 cttcagatct tcttcaatac gcatgtgcgg attcctgggg ttaatggcta taagggtaag
    36181 agcgcaactc cagtgacgct atcatacgca ctaatcaatg cggcgcaaga ctgcgaaatt
    36241 gtcttttttt acgctacaat cccataaatt tacctggtaa atagtgagcg aacgtttcat
    36301 caatttcact ttcgcgcgct catttttaac gttttgataa tcatgatgaa ttcccgggaa
    36361 tataaattat ggggtatata gttgccttaa cgggaggcat tggcagtggt aagagtaccg
    36421 ttgccaatgc gtttgctgat ctcggaatta acgtcattga tgccgatatt attgcgcgtc
    36481 aggtggttga accaggtgca cctgcgctac atgccattgc tgatcacttt ggcgaaaaca
    36541 tgattgctgc tgatggaaca ttgcagcgcc gggccttgcg cgagcggatc ttcgccaacc
    36601 cggaagagaa aaactggctt aacgacctgc tgcatccgct gattcagcaa gagacgcaac
    36661 accagatcca gcaagcaact tccccctatg tactgtgggt tgtgccattg ctggtagaaa
    36721 actcactgta taaaaaagcg aatcgagtgc tggtggtgga tgtcagccca gaaacgcaac
    36781 ttaagcgcac catgcagcgc gatgatgtaa ctcgcgaaca tgtcgaacaa atccttgctg
    36841 ctcaggcaac gcgcgaagcc cgccttgccg tggcagatga cgtcattgat aataacggcg
    36901 caccggatgc tatcgcatcg gatgttgccc gcctgcacgc acactatttg cagcttgcgt
    36961 cgcagtttgt ctcacaggaa aaaccgtaat gcagacccag gtcctttttg aacatccact
    37021 aaatgaaaaa atgcgtacat ggctgcgcat tgagtttttg attcagcaac tcaccgttaa
    37081 tttacccatc gttgaccacg ctggcgcgct gcatttcttc cgtaatgtca gtgaattact
    37141 ggatgttttc gagcgcggcg aagtccgcac tgagctgttg aaagaactta accggcagca
    37201 acgtaaactc cagacctgga ttggcgtgcc tggcgtggac cagagccgta ttgaagcatt
    37261 aattcagcag ttaaaagcgg cggtgagcgt attaatttcc gcgccgcgta tcgggcaatt
    37321 tctgcgtgaa gatcgtttga ttgctctggt gcgtcagcga ctgagcatcc caggcggctg
    37381 ttgcagcttt gatttaccta cattgcacat ttggctgcat ctaccccagg cgcagcgcga
    37441 cagccaggta gaaacctgga ttgccagcct gaacccgctc acccaggcac ttaccatggt
    37501 gctggattta attcgccagt cggccccctt ccgtaaacaa accagcctga atggttttta
    37561 tcaggataac ggtggcgatg ccgacttgct gcgcctgaat ctgtcgctcg attcacagct
    37621 ttatccgcaa atttccggac ataagagccg ttttgccatt cgttttatgc cgctggacac
    37681 tgaaaacgga caggtaccgg aacgtctgga tttcgaactg gcctgttgct aaggagtaaa
    37741 aatgtcagaa actattacgg tgaattgccc aacctgcggg aaaacggtgg tgtggggtga
    37801 aatcagcccg tttcggccat tttgctccaa acgttgtcag ctgatcgacc tcggagaatg
    37861 ggctgctgaa gaaaaacgaa tccccagcag tggcgatctt tccgaaagcg atgactggag
    37921 cgaagagcca aagcagtgac atttgtctga tgcctcacgt aggcctgata agacgcggac
    37981 agcgtcgcat caggcatctt gtgcaaatgt cggatgcggc gtgaacgcct tatccgacct
    38041 acagacgctt aagcttcgca attaccggtt cattggctgg cggaaaatca tcggcattaa
    38101 gaccgaccag cgacatccac tcaccgggtt gcccttcttt accccacggc accccttccc
    38161 aactttcgac cagccagaac cacagagtta tatgcctgtc cgggaattca tattccagtt
    38221 tttcaaatag cgaaaaatgt tgtggggtaa tcccgacctc ttcctgaagt tcacgcacca
    38281 ccgcctgttc cggcgtttcg cccatttcaa ttttaccgcc gggaaactcc agtttattcg
    38341 ccatgtgcgc atctgctgcg cgacgtgtta taaagatttc attgttctcg ttgcgaataa
    38401 tacctaccgc aatttgcagc tttttcattg tcttaaacct ataaaaaagg cgcagaatcc
    38461 tgcgcctttt acttcaactg ttagctttta ttgcaggcga ccatggcact gcttgtattt
    38521 tttaccagaa ccgcacgggc aaggatcgtt acgtcctact ttgcgttcac cggtttgcgc
    38581 cgccagtgca gctgcggctg cagagtcgtc atcctgatgg ctaagctgct gcatttgtgc
    38641 taaacgctcg gcttccatac gacgctgttg ttccagctcc tcaacctctt caggcatacg
    38701 tacctgaact ttgctcagcg tactgataac ttcatatttc aacgactcca gcatcgctgc
    38761 aaacatggag aacgattcac gtttgtattc ctgcttcgga tctttctgtg catagccacg
    38821 caggtggata ccctgacgca gatagtccat cgctgccagg tgctctttcc acagggagtc
    38881 aagcgtttgc agcatgacgc ctttctcgaa gtgacgcatc atctcagcac caaccacttc
    38941 ttctttacgc tgatacactt cgatggactg cgccagaatg cgctcacgca gcgtctcttc
    39001 atgcagttct ggttctttat ccagccactc ggcaattggt aaatcgaggt cgaaatcgtt
    39061 cttcagacgt tcctgcagcc ccggaatatc ccacatttct tccagcgact gtggcggaat
    39121 gtaggcatca atggtggctt tgaacacatc ttcacgaatg ctgttgatgg tttcgctcac
    39181 atcgctgaca tccagcagtt cgttacgctg ggagtaaatg gcgcgacgct gatcgttagc
    39241 cacgtcatca tattccagca gttgcttacg aatgtcgaag ttacgacttt caactttacg
    39301 ctgggcgttg gcaatcgctt tagtcaccca cgggtgttca atggcttcgc ctggcttcat
    39361 acccagttta cgcatcatgc cggatactcg gtcggaagca aaaatacgca tcagcgcatc
    39421 ttccatcgac aggtagaaac gggaagaacc agcatccccc tgacgaccag aacgaccgcg
    39481 caactggtta tcgatacgac gggattcgtg acgctcggta ccgatgatat gcaggccacc
    39541 tgcttccagt accgcatcgt gacgtacctg ccagtcggct ttaatttttt caatttgctc
    39601 tgcggtcgga ttttccagcg cggcaacttc tgcctgccag ctacctccga gcacaatatc
    39661 tgtaccacga cccgccatgt tggtcgcgat agtcaccgca gccggataac ctgcctgagc
    39721 aacaatcgcc gcttcgttgg cgtggaattt ggcgttcagg acattgtgct taataccggc
    39781 tttggtcagt tcgtttgaca ccagctccga tttttcgatg gagatagtac ccaccagcac
    39841 cggctggcct ttcgcagtac gttctttgat atcttcaatg atcgcctgaa ttttttccgc
    39901 ttcagtcatg tagaccaggt ccggcagatc tttacgaatc attggacggt tggtcggaac
    39961 aacgacggta tccagtttgt agatggagct aaattcgaaa gcttcggtat cagcagtacc
    40021 ggtcatcccc gccagttttt catacagacg gaagtagttc tggaaggtga tcgaagccag
    40081 cgtttggttt tcgttctgga tctgcacacc ttctttcgct tccacagcct ggtgcagacc
    40141 atcggaccag cgtcggccct gcatggtacg accggtgtgt tcgtcaacga tgataacttc
    40201 accatcttta acgatgtagt cgacgtcacg ggtaaacagc gcatgagcgc gcagcgccgc
    40261 cgttacgtgg tgcatcagca tgatgttggc cggagagtac agagactccc cttcatccat
    40321 gatgccctct ttcaccagca gttcttcaat cagcaccaga ccacgttcgg tcaggttcac
    40381 ctggcgagat ttttcgtcca ccgagaagtg gccttcgccc tggaaggttt cggagtcttc
    40441 tttttcctga cggatcaggt gcggaataat tttattcacg cgtttataca tttccgagct
    40501 gtcttctgcc gggccggaaa tgatcagcgg tgtacgcgct tcatcgatca ggatggagtc
    40561 cacttcgtcc accagcgcat agtgcagttt acgctgtaca cgttcttcag ggctgaacgc
    40621 catgttgtcg cgcaggtagt caaagccgta ttcgttgttc gtaccgtaag tgatgtcagc
    40681 tgcgtaagct tcgcgctttg ccggtgctgg catgcccggc aggttgatac caacagtcag
    40741 gccaaggaat tcaaacagcg gacggttgtt ttcggcgtca cgttgcgcca ggtagtcgtt
    40801 gacggtaact acgtgcacgc ctttaccggt tagtgcgttc aggtaagcag gcagcgttgc
    40861 ggtcagggtt ttaccttcac cggtacgcat ttcggcgatg cagcgttcgt taagaaccat
    40921 accgccgagt aactgaacgt cgaagtgacg cataccaaag acgcgcttac ttgcctcacg
    40981 caccacggcg aaagcttccg ggatcagatt ttccagcact tcgccttttt ccagacgcgc
    41041 acgaaactct gcggttttcc ctttcagttc ttcatcggag agtttttcca tctccggttc
    41101 catggcattg atgatgttga ccactttgcg catccggcgc agggtgcgat cgttacgact
    41161 accgaaaact ttagttaaca atttgattag cataataaaa tctcaaacgc cccgcgttac
    41221 ggagttaata aaatgaagta aaggtttatt gttgttaggt gaggcgttga gggccagcac
    41281 ggatgccttg cgcctggctt atccagacgg gcgtgctgaa ttttgcttgt ggggtaaaat
    41341 gcgcataatc aatgcgataa cccttttcag acggcgtgcc ttcctgggtc agcagcgcgc
    41401 tgagcgtatc cagtaatgca agatgttgcg cctgaagagg caaagattct tcagcaacgg
    41461 gcagtgtttg cggtgccatt gcgaaagaaa gatgacggat taccgtacga atagcatgtt
    41521 gatgccagta atcaacggaa tagttcgaat tcgggcggcg cgtgttcgct tccagcaagg
    41581 ccaattgacc aaagttaact ttggctgaag gctcgtggtt gcgggttgtc gcttttgcgg
    41641 gcgcgtttgg ttcggcggcg ttgctgagcg caggcaaacc taaactcgcc gcgaccatcc
    41701 ctaataagag atgcggccag aagtagcgtt taccaaactg tcgccagcgc gtcagtattc
    41761 cactcacgtt attgccatcc cgatcgaatt caaacgtatt tagcgcacaa atcttatcat
    41821 taaagcctga agtccacagc acgaatgaag ataagttgtg cgtaaaagtg cttaagaaaa
    41881 cagcgttcgc accgtttttt atagctctta taaaattcaa aaaaaaacga ctggttcacc
    41941 tggccggaga gagtgccaga tttgccagtc gaattttata cgacagtata aatgtcgtta
    42001 tgccagtaca gctgaaggcg ctttgaaggc caacggcagt tctgcgtcgt cctggaaggt
    42061 cacatattcc caggcttcct gtttcgccag gacagcctgc agcagtttgt tattcagtgc
    42121 atgaccggat ttataagcgg taaatgcacc aataatattg tgaccacaca tgaacaagtc
    42181 accgatcgca tcgagcattt tgtgacgcac aaattcgtct tcaaaacgca ggccgtcttc
    42241 gttcagtacg cgataatcgt caacaacgat ggcacaatcg aagctgccgc ccaggcacaa
    42301 accacgggac tgcagatatt cgatatcacg catgaaaccg aacgtacgcg cacggctgat
    42361 ctggcgcata aacgcatcag cggagaagtt catcgcatag cgctggttgc tggaatcaat
    42421 agccggatgg ttaaaatcga tggtgaaatc cagcgaaaaa ccattgtacg gcttaaattc
    42481 agcccactta tcgccatctt cgacacgaac agtctctttg atgcgaacaa attttttggc
    42541 gcagttcaac tcgtcgatac cggcgtcaag cagcaggtat acaaacggag cggcgctgcc
    42601 gtccatgatc gggatttccg gcgcgttaac ttcgataaca atgttatcga tgcccaggcc
    42661 cgcgagagca gcattgaggt gctctacggt tgaaatccgt acatcatgct cgttgaccag
    42721 acacgtacag agcatggtat cacgcacaga tttggcatcg gccgggaaat ctaccggtgg
    42781 attcaagtcg gtgcgacgat agatgacccc ggtgttggcc ggcgcagggc gtaacgtcag
    42841 ggtgactttc ttgccggtat gtaaaccgac acccgtcgcc tgaacgatac gtttaagtgt
    42901 cctttgtttg atcatcgtat tatctcgcca aattacctat ccaaccgaag tgtactatac
    42961 attcggcggg ccagtttagc acaaagagcc tcgaaaccca aattccagtc aattcttaat
    43021 cagcttgctt acgtaggaat gctgggatat ccagataatc cggctctttc gcagtttgcg
    43081 gcgcattgtc attcactact ttagcaaccg gcttctgctc ctgggtcagc ggagccatcc
    43141 catgctgctg gtagcgatcc atcactggct gctgaacctg cttattggtc accagagtga
    43201 tttcaggacg tttgtccatg ccgatacctg tcgcaacaac ggttacgcgc agctcgtcat
    43261 tcatatccgg gtcaagagaa gtaccgataa ccacagtcgc gttgtcggaa gcaaatgcac
    43321 ggatggtgtt acctaccgtt tcgaactcat ccagacgcag gtcgaagccc gccgtgatgt
    43381 taaccagcac gccgcgcgcg ccagacaggt cgatatcttc cagcagcgga gaagagatag
    43441 ccatttcagc agcttcttcc gcacggtctt caccgctcgc cacgccagaa cccatcattg
    43501 cgtagcccat ttcagacatt acggtgcgta cgtctgcaaa gtccacgttc atcaaacccg
    43561 gacgagtaat cagttcggcg ataccttgca cagcgccttt cagtacatcg ttcgctgcgc
    43621 caaacgcatc cagcagggag ataccgcggc ccagaacttt cagcagtttg tcgttcggga
    43681 tagtgatcag agagtccaca tgcttggaca gttcagtgat cccctgctcg gcgaatgcca
    43741 tacgcttctt gccttcaaag ttgaaaggct tagtgacgac agcaacggtc aggataccca
    43801 aatcttttgc cacttcagcg acgactggtg ctgcacctgt accggtacca ccacccatac
    43861 ccgcagcaat aaagaccatg tctgcacctt ccagcgccgc acgcaatgca tcgcgatcct
    43921 catcagccgc attgcggcca acttctggat tagcgccagc gcccagtcct ttggtgatac
    43981 cgctaccgat ttgaatcgtc tgtccaaccg ctgttttacg cagcgcttgt gcatcggtat
    44041 ttaccgcgaa gaattcaaca ccttcaatac gctcgcgcac catgtgttca acagcattac
    44101 cgccgccgcc gccgacgccg atgactttaa tcaccgcgtc attggtaagt tccattggtt
    44161 caaacatagt ttctctccga tttgtgcctg tcgcctgagg ccgtaatcat cgtcggcctc
    44221 ataaaaatta aaactctttt cgcagccaac tattgagtcg cttgatccac gagccaactg
    44281 atgctgtaac acgtttttct acttcagctt caccgttaag atgtgactct ttcccatagt
    44341 gaagcaatcc caccgccgtc gaataatacg gctcctgagc ataatccgtt aaaccggtaa
    44401 tgttcagcgg cgcgccgata cgcacttgcg tatgaaacac gcgctgagca caggctgcaa
    44461 gaccttcgat ctgcgctgcg ccaccggtta atacaatgcc tgccgccagg tgatgtttaa
    44521 ccccttgttg gcgaagcttt tcctgcaact gcaatatctc ttcgttgacc aggttgagca
    44581 actcggtata gcgcggctcg atcacctctg ccagggtctg acgttgcaga ctccgcggcg
    44641 gacgaccgcc tacgctcggc acttccacgc tctcatcttt tccaacgatg gaacccagcg
    44701 cacaaccgtg gcgaacttta atcgcttcgg cgtcgcttgg cggcgtgcca aaggcgtaag
    44761 cgatatcact ggtcacgaca ttgccagcat aaggaattac ctttgtgtgg cgcaatgccc
    44821 caccggtata aacggcgata tccattgtac caccaccgat atcgacgacg cagacaccca
    44881 gttcacgttc atcttccgtc aataccgaat aacttgatgc cagtccggca aatatcagtt
    44941 ggtcaacttt cagcccacaa cgttcaaccg ctttgacgat gtttttcgcc atatcgttgt
    45001 gacatgtgat caggtgcact tttgcctgca tccgcacacc cgaaagtcct accggattct
    45061 tgatcccttc ctgatagtca atcgcatact cttgcgggat cacatgcagc acacgatgct
    45121 catcgcgcac acgcaccgat ttcgcggtat ggacgacgtt ttccacatct tcttgcgtca
    45181 cttcttcttc agaaataggc accataccaa tttcattctg gcagctgatg tgcttaccag
    45241 aaagcgccag atataccgaa gagatctgac aatctgccat caattctgcc tggtcaatgg
    45301 cgcgttgtac gcacttgacc acggattcga ggtcgttcac cccgccttta tccataccac
    45361 gtgacgggca gctgcccacg ccaatgatat tgaccatacc gtcgggcaga acttccccta
    45421 ctaaagcggc aaccttcgcg gtaccaattt ccagtcctac taccagtttt ctgtccgtcg
    45481 ccttgatcat tgttgttctg cctgtgcctg attttgttgc tgagtagatt cctctggcgg
    45541 caagggcgcc cagcctactg ccgctccaga gtcataacgc aaatcaacgt agctaatccg
    45601 tttgccatcg gtttgcgcct gctgctgtaa aaccggataa agttctacaa agcgaaccaa
    45661 acgtttcatc gtatcgcccc ggccaagatt gagcttaata tcgttattca gcgtcaactg
    45721 ccaggaacgc cgcgcggtca tcgccgcttc cttcagagta aatctgtcct ttgccagcat
    45781 ctgccccatt tcgcgatagc cctgcaacac ttcattagcg ctgccttccg ggccatacag
    45841 cattggaagc acctgcttgc tggtgcgatc tggcggcacg ctgaaggtgt ttccttccgc
    45901 gtctaccatg tgttgatcat tccaccgcgc aatcggcaca tattcaacca gatgaatctt
    45961 caattcatca ggccactgct ttctgacgct cacctgctta atccacggca ggcgttgttc
    46021 tatttgcgtc tggatgatgt tgacatcctg ggtcataaag gtacccggct cacccaatgc
    46081 caggatcgac tgccggatat cgtcattacg cgtgtaatgg cgttcaccgg tcaacaccag
    46141 ctttgagagc ggcaggcgtt gcgcatcttc catccagctc aacacgaccc agccgctcac
    46201 caacactgtc gttaaaacgg tcagcaggaa aaggatcccc gccagacgcg ttccattatt
    46261 gcggcgagaa gaaacctctt cttcgctgtt tcgcgtgttc agagcagcct gcgacatatt
    46321 agtccgccag ttccagaatt cgtactacca actgcgagaa gctcatacct gcctgacgtg
    46381 ccgccatcgg caccaggctg tggctggtca tacccggtga ggtattggct tccagcagat
    46441 aaaactgtcc atcactgtcc agcataacgt caatacgtcc ccatcctttg caacctaacg
    46501 tcgtccatgc tttcagcact aatgcctgca aattggcctc ttgtgacgct tccagacctg
    46561 cggggcagaa atactgtgtc tcatcagaga gatacttcgc ctcataatca tagaaggttc
    46621 cggacggttg aatacgtatt gacggtaaaa tttcttcacc gagtatcgca accgtgaact
    46681 ccggcccact tagccatttt tcaatcaata cttcttcatc gtgctgaaat gccaatctta
    46741 atgcatcttg tagagcattt tctgctacta cttttgacat tcccacactg gaaccttcgc
    46801 ggctcggctt aacgataacc ggcaaaccca gagcagaaat ttctgctaac tgcttatcgc
    46861 tcaggccttt ttcaaactct gcgcgggtta acgctaccca cggcgcgacc ggtaaaccgg
    46921 caccttgcca cagaagtttg ctgcgtagtt tatctattga aagcgcagat gccatcactc
    46981 cgctgccggt ataaggcaag cccatcagct cgagcatgcc ctgcagcgta ccatcttcac
    47041 cgccgcgacc gtgtagcgcg ataaacactt tctgaaaacc catcgacttc agttgcgtca
    47101 cgtcgacttc tttcgggtcg ataggatacg catcaatacc gccttcacgc agcccggcta
    47161 acaccgctgc gccagaattc agagaaactt cccgctcagc ggaagtcccg cccaacagga
    47221 ccgcgatttt atcagtcatg ttgttcttcc tccggagttt gcggcttcag tttgatttca
    47281 gctaaagaac gggcaatttt tccgatatta ccagccccct gaacgagaat caggtcgtta
    47341 ccggttaata ccggtgccag catctcggct acctgcgcag ggtccggcac cagaatggga
    47401 tcaattttcc cacgtccacg aattgtgcga cacagcgaac ggctgtccgc tcccggaatt
    47461 ggcgcttcgc cagccggata cacttccagc atcaacaggg tatcaacctg cgtcagcaca
    47521 ttggcgaaat catcatacag gtcgcgcgta cgggtaaaac ggtgcggctg aaacagcatt
    47581 accaggtttt tatccggcca gcctgcgcgc gccgctttaa tggtggcgtc aacttccgtc
    47641 gggtggtggc cgtagtcatc gaccagcatt gccgtaccgc ttttaccatt cactggctcc
    47701 agcgggaatt caccgaggaa atcaaaacgg cgaccagtcc cctggaagct ctccagcgca
    47761 cgcaaaatag cctcgtcgtc aattccctct tccgtagcaa ccgcaaccgc agctgcggcg
    47821 ttcagcgcgt tatgacggcc tggcgcattc agggtgacgc gcatcggctc tttgtcctga
    47881 cgcagcagcg taaagtgccc ctgcgggcca atctgctgat aatcttctac acgcacgtcg
    47941 gcatcttcgc tgaagccgta agttgtggtc tgacgtccca cacgcggtaa caattcgcgg
    48001 atcaccggat catcaacaca catcaccgca cgaccgtaaa aaggcaggtt gtgcagaaaa
    48061 ttaataaagg tctgttttaa attctcaaag tcgccctggt aggtatccat gtggtcggct
    48121 tcgatattgg tgacaatcgc caccatcggt tgcagatgca ggaacgatgc atcactctca
    48181 tctgcttcgg caatcaggta ccgaccatgc cccaaacgcg catgaacccc cgccgctttt
    48241 accagcccgc cgttaacgaa ggttgggtcg agccccgctt ctgcgtagat gctggaaacc
    48301 atcgcggtag ttgtcgtttt gccgtgcgtt cctgcaatgg cgatgccatg acgaaaacgc
    48361 attaactcag ccagcatttc ggcacgacgg atcaccggaa tacgcgcttc atgagctgcg
    48421 acaatttccg ggttgtcggc agaaatggcg ctggaaacaa cgaccacgct ggcatcacgt
    48481 acgttttccg ggcgatggtt gaaataaatc gtcgcaccca gattcagtaa ctgctgcgtg
    48541 accggatttg gcgctaaatc ggaaccactg atctgataac cttcattggc cagaacttcg
    48601 gcaataccgc ccataccggc accaccaatg ccgacaaaat gtatgtgccg aacgcgacgc
    48661 atttcgggca cgatggaacg cagttttgcc aattgttgtg tattcattct ttacgccatt
    48721 aacttcttaa attcatacga tgcaaaaggc atcgctacaa ttacgcccgg gcagcccggc
    48781 tcacttcatt tgccactcgc tcggtggcat ccggaatgga tgcagcgcgg gcgcgttctg
    48841 ccatggttaa taaggtttct cgcgaccacc cggccagggt gttggcgaca gcatccacgc
    48901 taagctgtgg ctgctcaata attttggctg cgcccgcttt ttccagcggt agcgcattcc
    48961 agtattgctg gcggtcttta tgttgaaacg gcacaaacaa cgccggtagt cctgccgcgg
    49021 cgatttcact cacagttaaa gcaccggagc ggcaaacgac gacatccgcc cacgcatacg
    49081 ccgccgccat atcatcaata aattcagtca ctttatgctg cggttgcccc gcttcggcat
    49141 acgcctgttc aacggattgt tgcgaacctt tgccgctctg atgccagata gtgactgaat
    49201 cacccagttt cgcagcaacc tgcggcattg tctggttaag aatgcgtgcg ccctgggaac
    49261 cacccactac caacacacga accggacctt cacgtccagc caaacgttgc tgcggcaacg
    49321 gcagcgccag cacatcggta cgcaccgggt tacccaccac ttccgcatta gggaaagcgc
    49381 ctggaaacgc ctgcatcact ttggtagcaa tcttcgccag ccatttattg gttaagcccg
    49441 caataccgtt ttgttcatga agtacaaccg gaatgcctaa cgaccacgcg gccagaccac
    49501 ctggacctga tacatagccg cccataccga gtaccacgtc aggtttgtac gctttcataa
    49561 tcgcccgcgc ctgacgccag gcgttgaaga tacgcagcgg cgcagctatc agtgctttta
    49621 taccttttcc acgcaaacca gagatacgaa tgaaatcaat ttcgatgcca tgttttggca
    49681 ctaagtccgc ttccatacgg tcggcagtcc ccagccagcg aacttgccaa ccctgagcca
    49741 ttagatagtg cgcaaccgcc agtcccggga atacatgtcc accggttccg cctgccatca
    49801 ccattaatcg ctttccttga ccactcatcg tgaacctcgt acaaacgcct gcgctttctc
    49861 cagacgcgtt tcataatcaa tacgcaacag catcatgatg gctgtcgaca taatcagtaa
    49921 gctcgaacca ccgtaactga ttagcggcaa tgtcagacct ttggtcggta acatccccgc
    49981 cgccgcgcct acgttaacca gcgcctggaa gctaaaccag atgccaatag aacaggcgag
    50041 aaaaccggaa aaacggtggt caatttctaa tgctttgcgg ccaatcgaca tcgcacgaaa
    50101 agcgacgaag aataccatta aaagtgctag taccacaccg acataaccta attcttcgcc
    50161 gataatggcg aaaataaagt cagtgtgcgc ttccggcaga tactccagtt tttgtaccga
    50221 attacctaaa ccttgccccc aaagttcgcc gcgaccaaac gccatcagcg attgcgttaa
    50281 ctgatagccg ctgccaaagg gatcttccca cgggttccag aatgcggtaa cacggcggat
    50341 acggtacggt tcggcgagga tcagcaacac aaccgctgaa atgcccatac cgataatggc
    50401 aatgaactgc cacaatttcg ctcccgccag gaacaacatc gccagcgtag tcacaaacaa
    50461 caccaccacc gtaccaaggt ctggctgtgc cagcagtaac actgccaaca ccagaatcac
    50521 gcccatcggt ttcaggaagc cgcgcaggtt attacgtact tcgtcgcctt tacgcaccag
    50581 atagttggcg atatagcaaa acagcgacag ttttgtcagc tccgcaggct ggatacgcag
    50641 caaaccgaga tcgatccaac gcgatgcccc tttaacagcg ctacccacta ccagcacgat
    50701 catcagcagg atgatagatc cgagcagcat cgtggcactg tagcgttgcc agaactccat
    50761 cggcagacgc agcgtaatga tcgccagaat aaacgccaaa atcagataga caccatcacg
    50821 cttcgcgaag aagaacggat cgttggttaa gcgttgccct atgggcattg acgccgaggt
    50881 caccatgata aagccaatcg ccgcgaggcc gaaggtcagc cacagtaagg tgcgatcgta
    50941 catgatcagg ctgtcggtat ctttttcccg cgagcccatc acccagccct ttagcgccgt
    51001 ggagatccag accaggatac tgaatcctgg caggcgcggc attttcaggc gagggagaga
    51061 taaacgcatc aacctaactc cttcgccaga cgggcaaact cattgcctcg ttgttcaaag
    51121 ttcttgaact gatcaaggct ggcacaggcc ggggagagca gaaccatatc gcccggctga
    51181 acacgcggag ccagcaagcg catagcctgt tccatagttt cggtttgttc tgccacttcc
    51241 gggcgtagcg ccgccagctg cgcgccgtca cgaccgaaac aatacagacg tacgttatcg
    51301 ccattcaggt aacgcaccag tgggctaaag tccgccgatt taccatcgcc gcccagcaac
    51361 aaatgcagcg tgccgtctac gtgcaggcca ttcagcgccg cttccgtact gccgacgttg
    51421 gtcgcttttg aatcgttaat ccagcgcaca ccgttatgct ccagcaccac ttcaaagcga
    51481 tgcggcagac cggtgaaggt ggttaacgct ttcaggctgc tggcgcgcgg taaccctgcg
    51541 gcatctgcca gcgccagcgc caccagcgca ttggtgtagt tatgctgccc ggaaagtttc
    51601 atctctttca cattcagcac tttctcgcct ttaacccgca gccaggtttc gccctgctga
    51661 tgattcaggt gatagtcacc catgttgacg ccaaagctga cgcagcgttc atccgcaccg
    51721 cgaatcggca ttgttaaggc atcatcagca ttaaccacgc aaactttcgc gttttcgtaa
    51781 atgcgcagtt ttgctgcacg atactgttgt aaaccaaacg ggtagcgatc catatgatct
    51841 tcagtcacgt tcagaatggt cgctgctacc gcctgtaagc tggaggtggt ttccagctgg
    51901 aagctcgaca gttccagcac gtacagttca cactcatcat ccagtagcat caacgcaggc
    51961 aggccaatat tgccacccac accaacgtta acccccgccg ctttcgccat ttcacccact
    52021 agcgtggtga ccgtgctttt gccgttagaa ccggtaatcg ccacaatcgg tgcttgtgct
    52081 tcgcgacaaa acagctcgat atcgccaacg atttcgattc cggcatcagc ggcagcgctt
    52141 aaggatggat gcgccagtgc aataccggga ctggcgacaa tcagatctgc cgccatcagc
    52201 cattcatcat tcagactgcc cgtgtggcgt tctacggctt cgggtaattt atccaggcca
    52261 ggcggtgtca tacgcgtatc cataacgcgc ggcgtcacac cgcgagcgag gaaaaagtcc
    52321 acgcaggaaa gcccggtgag gcccaggccg ataatgacaa catttttacc ctggtaatca
    52381 gccatgatta acgtaccttc agcgttgcca gaccaatcag gaccagcatc agcgaaataa
    52441 tccagaaacg cacaatgacg cgcggttccg gccagccttt cagttcatag tggtgatgaa
    52501 tcggtgccat gcggaaaata cgttgtccgc gcagtttaaa ggagccgacc tgcaggatga
    52561 cagaaagcgt ttctaccacg aacacgcccc ccataatcac cagcaggaat tcctgacgta
    52621 gcagtacggc gataatgcct aacgcaccac ctaacgccag cgaaccgaca tcgcccataa
    52681 agacctgcgc cggataggtg ttaaaccaca ggaagcccag tcctgccccg actatcgcgg
    52741 tacagacaat aaccagttcc ccggcgtgtc gcagatacgg tatgtgtaaa tagctggcaa
    52801 agttcatgtt gccggtcgcc cacgccacca gcgcaaaacc accggcgaca aatacggtcg
    52861 gcataattgc cagaccgtca agaccatcgg tcaggtttac cgcgttgcca gtacccacaa
    52921 tgacgaagta agccagcaga atgtagaaca gccccagttg cggcatcaca tctttaaaga
    52981 atgggaccac cagctgcgtt gcgggcgtgt ctttgccggc aaggtacaaa gcgaaagcaa
    53041 cacccagcgc aatgaccgac atccagaaat atttccaacg agcgatcaac cctttggtgt
    53101 ctttacgcac cactttgcga taatcatcaa caaagccaat aacaccgtaa cctaccagca
    53161 ccaccaacac gcaccagacg tacggattgg acgggtaagc ccacagcagc acggagatca
    53221 caatcgctgt caggatcata atcccgccca tggtcggcgt accgcgcttg ctgaagtgtg
    53281 attcaggacc gtcgttacgc actacctgac caaaggaaag tttttgcaaa tgagcaatca
    53341 tgcgcgggcc catccacaat gagatgaaca gcgcggtcag caggctgacg atggcgcgaa
    53401 acgtcagata ggaaaagacg ttaaagccgg aataatattt gaccaaatgt tcggccagcc
    53461 aaactaacat gtcccattct cctgtaaagc gcgtactacc tcttccatgg cagcactacg
    53521 tgaaccctta actaaaatcg taattacctg ttgctcagca atcagtgatt taagacgcgt
    53581 aattaacgca gttttatcag caaaatgttc gccaacgccg ctggcggtgc tgatagcatg
    53641 gctttgttta cccatgctta acacgcggtc aataccagca gcttttgccg cctcacccac
    53701 ctgtacatgg caggcttcgc tttcagcgcc cagttccgcc atatcgccca ccaccagcac
    53761 gcggtagccc ggcatttcag ccagtacctg aacagctgcg gtcattgaac cgacattggc
    53821 gttgtaggag tcgtcgagca gcaactggtt ttctgccagt tggatgggga acagacggcc
    53881 aggaacagct ttcagatttg ccagccccgc tttgatagca tcaagcgttg cgcccacgga
    53941 catggagagc gcagcggctg ccagcgcatt cgcaatattg tgacgccccg gcaacggcag
    54001 cagaacatcg acgctaccgg ttggggtttg tagggtaaat tccataccgt gcgaggtcac
    54061 atggatattg gtggcggtga aatcgctgtt ggcggcattg ggtgagaaac gccacacttt
    54121 gcgtgagcca attacgctct gccagttcag ccagtcgttg ttgtcggcgt tcataatggc
    54181 gataccgttt tccggcaggc cgctaaagat ttcacctttc gctttcgcga cacccgcaag
    54241 cgagccaaaa ccttccagat gcgccgctgc caggttgttg accagcgccg cttccgggcg
    54301 agtcagactc acagtccagg caatctcgcc ctgatggttc gcgccaagtt caataactgc
    54361 gtaatcgtat tccggcgtta agcgcaacag cgtcatcggt acaccgatgt cgttgttgag
    54421 attgcctgcc gtataaagcg tgttgccgca ctggcttaaa atcgccgccg tcatctcttt
    54481 aacggaggtt ttgccggagg accccgtcag agcaaccacg cgcgccggaa cttgctggcg
    54541 aacccatgca gccagttcac caaacgccag acgcgtatcc ttgacgatta actgcggcag
    54601 gtcgatgtcc agcggacggc taaccagtag tgcgcctgcg ccgccagctt tcgcctggtc
    54661 ggcaaaatcg tgggcatcaa aacgttcacc tttcagggca acaaacaggc agcccggcgt
    54721 cagttttcgg gtatcagtgg ttacagcatc aagggtgata tctgcacctt gcagttcacc
    54781 gttgagaata tcggtaagtt ggctaagggt tacgctaatc atgcaatccc ccccagcaga
    54841 cgcgccaccg tgacgcgatc ggagtagtcc agacgctgat tgccaacaat ctggtaatcc
    54901 tcatggcctt tgcccgcgac cagtaccaca tcattctctt tagcctgcat aacggcgcaa
    54961 gtcaccgctt cagcacggcc ttccatcact ttggcatgtc cggcatctaa cattcccgcc
    55021 agaatatcgt tgatgatggc acgcggttct tcggtacgcg ggttatcgtc cgtcaccacc
    55081 gccacgtcag caaactcttc ggcaattgcg cccatcagtg gacgcttacc tttatcgcga
    55141 tcgccaccac agccaaagac gcaccacagc ttgcccgcac agtgcaggcg cgccgcctgt
    55201 aaggcttttt ccagtgcatc cggcgtatgc gcgtaatcca ccaccaccgt cggtttgcct
    55261 ggcgcagtga acacttccat acgtccgcaa accggttgca gacgcgcggc ggttttcagc
    55321 agatcagcca gtggatagcc cagcgccagc agtgttgcca gcgcgagcaa caggttgctg
    55381 acgttaaaag cgcccatcag atggctttca atttcgccat cgccccagct tgaactaaag
    55441 cgaatggtcg cgccgctgtc gtgatagttc acttcggtcg ctttcaacca gcgtccgtga
    55501 cagttcggat tgatatgatc ttccattgat accgcaaccg cgtccggcag ttttgccagc
    55561 cagcggcggc ccacttcatc gtcggcgtta ataatcgcct gaccgcaatg atgttcagaa
    55621 taaagcagcc atttcgcagc ttcgtagtgt tccatatcac catgataatc aaggtgatcg
    55681 cggcttaagt tggtaaagac cgacgccgga aatttcaatg ccgccacacg gtgctgtacc
    55741 agcccgtggg aggaaacttc cattgcgcaa aaagtcgcgc cctgatccac cagccccgcc
    55801 agctcatgct gaacatcaac tgccgaaccg gtagtatttt ctgtcgggat cactttcccc
    55861 agcagaccgt taccaacggt gcccattacc gcgctggttt cgccaagcag ttggctccac
    55921 tgcgccagaa gctgggtcgt cgtggttttg ccgttggtgc ccgttacgcc tacgagacgt
    55981 aaattgtcag agggttcatg gtaaaagcgg cccgccagtg cagataaacg ctcgttgagc
    56041 tggctgagat agatgaccgg tacgccgtgc atttcacgga tttcaccgtc ggtcgcctca
    56101 tctttcgcct ctgcaataat ggcagccaca ccttgcgcta tcgcctgcgg gatatatcga
    56161 cgcccgtccg cctgatgacc tactacagct acaaagagat cgcccgccgc agccacacag
    56221 ctgtcgagtg tcatctctcg cagtgctcgc gaaggtgcgt ctggcaccca cggagcaaga
    56281 aggtcgcgca aattacgatc tgccacctgt cccctcgcct tgattaatca caaattcatt
    56341 tttatcgccc gttgtcagcg catccggctc gatgttcatg gtacgcaata cgccgcccat
    56401 gatggcacca aagaccggcg cggaaacggc gccgccgtag tatttacccg cctgcggatc
    56461 gttgataaca acaaccagcg cgaagcgcgg ctgactcgca ggcgcaacgc ctgcggtata
    56521 agcaatatat ttattgatgt agcgaccgtc cggcccgacc tttttcgcgg taccggtttt
    56581 aatggcgata cgatagcctt taatcgccgc cttcacgccg ccgccgcctg gtagcgccac
    56641 gctttccatc atatgcacca cggtgcgaac aatggattcc gggaagacac gttcaccggg
    56701 aaccgggggg tcaactttgg taatcgacag tgggcgataa atgccgtagc tgccgatagt
    56761 tgcgtagact cgcgctaact gtaacggtgt taccattagc ccgtagccga aagagaaggt
    56821 ggccctctct atgtcagacc accgttgttt ttgaggatat aagccactgc gttctccgac
    56881 caaccccaaa ttggtcgctt ttcccagtcc aaaacgtgag taagtatcta ctaacgctga
    56941 ggacggcatc gttaacgcca gcttggaaac accgacgtta ctcgacttct gtaatacccc
    57001 ggtcagggtt aattcgctgt agcgtgccac gtctttgatt tcgtggccgt taattcgata
    57061 aggaatggta ttgagtaccg agttttcccg caccacgcca cgttgcaacg cggtcatcac
    57121 caccatcggt ttaaccgttg agcccggttc aaacacgtcg gtgatggtac ggttacgcat
    57181 cgactctttc ggcgtgccgc tcagattgtt agggttgtat gacgggctgt tagccatcgc
    57241 cagcacttca cctgtgttga catccaccag cacggcgcta ccagattcag ccttgttaaa
    57301 ggccaccgcg ttgttcagtt cgcgataaac cagcgcctgc aggcgttcat caatactcag
    57361 cgccaggttg tgcgctgcct ggctgtcagt agaagaaata tcttcaatta cgcgaccata
    57421 gcggtcttta cgcacaatgc gctcacccgg ctgcccggta agccatttat cgaaactctt
    57481 ctcaacgccc tcaatccctt gactatcgac gttagtaaag ccgatgaggt gagcagtcac
    57541 ttcgccggac ggatagtaac ggcgagactc ttcacgcaga tgaatccccg gcagtttcag
    57601 ttttttgatg tagtccgcca tgtcagagtt tacctgacgc gccagataaa taaagcgccc
    57661 tttcgggttg gcgttaatgc gggcagaaag ctgatccagc ggaatattga gcgcgttagc
    57721 cagcgccttc cagcggtcac cgacgctgat accgccagcg tcatgcactt ctttcgggtc
    57781 agcccaaatc gcttttaccg gcacgctcac cgctaacggg cgaccagaac ggtcagtaat
    57841 catgccgcgg gaggtggaaa cttgctgaac gcgaagagaa cgcatgtcgc cctctttcac
    57901 cagcatatcc ggggagataa cttgtaacca cgctacgcgt ccgagcagaa aagccagcgc
    57961 caggagaata cagccgcata acaacgcaaa acgccaactg ataaagttgg catgttcttc
    58021 ctgacgtttt ggtttctgcg ttttcgccgc tgctttcatg cgtcgcgttt atccttattt
    58081 ttgcactacg atattttctt gtgacggatc aacatgctgc atttgcagct tttccgtggc
    58141 gatcctttcc acccggctat ggtcgccgag cgcattctct tcaaggatca ggttgcgcca
    58201 ttcaatgtct aaagcatctc gctccagcac cagttgttcg cgctgagcgg tcagtaaacg
    58261 ggtatggtgc gccgtggtta ccacggtcac cgccgtcaaa ataatgcaaa tgaacaggca
    58321 gagtggcagc ttcccaaatc gcaaaagatc gtcaccgata acaccaggca atgcatggcg
    58381 ctcgtggctt cccatcgatc ctttaacttt gcttagagct tctgtcactc tgctgatcat
    58441 gcgttcgtcc tctctgcaat acgcagaact gaactacggg cacgagggtt ctcagccacc
    58501 tcttcttcgc ccggcattaa cttgcctagt gctcgcagct gacggccacc cagttttttg
    58561 agctgctctt cagtcatcgg taaccctgcc ggaacttgcg gaccgcggct gttttcacgc
    58621 ataaaacgtt tcacaatacg gtcttccagc gagtggaagc tgatgatcga aagccgccca
    58681 cccggggcca gcacgttgag cgagcttttt agcgcctgct ctatctcctc cagttcactg
    58741 tttacccaaa tgcgcaccgc ctggaaggta cgggtcgcgg gatgtttaaa cttatctttc
    58801 accggcgttg cagcagccac gacttccgcc agttctttgg tgcgggtcat cggctgttcg
    58861 cggttacgct cgacaatggc gcgggcaatg cgtttggcaa aatgctcttc accataggtt
    58921 ttcaataccc aggcgatatc ggcttcttct gcggtttgta gccattcagc ggctgactgc
    58981 ccacgggttg ggtccatacg catgtccagc ggaccatcgc gcataaagga aaagccgcgt
    59041 tcagcatcat caagttgcgg tgaagagacg ccaagatcga ggagaatgcc gtcgatcttg
    59101 ccaataagat cgcgctcggc aacgtattcg cccagcgcgg agaaaggtcc gtggatgatg
    59161 gagaagcgcg gatcatcaat agtcttcgca acggcgatag cctgcgggtc gcgatcgatc
    59221 gccagcaaac gcccctcttc gccaagctgc gagaggatca gacgtgagtg accaccgcga
    59281 ccaaaagtcc catcaatgta gatgccatca ggacggatat tgaggccatt aacggcttca
    59341 tccagcagca ccgtagtatg tttatagttt tccatcattt tatagagaca agtcctgcag
    59401 tcgctccgat aagtctccgg tagccaactg ctctgcgtcg atatcttcct tgacctgttg
    59461 atgccaggtt gtttcatccc acagctcaaa cttgttgaac tgtccaacca gcatcacttc
    59521 ttttgtcagc ccggcatgtt gccgcagtac tggcgcgatt aacaatcgac ctgcgccatc
    59581 catctgacat tcgctggcat gacctaacag taggcgctgc acacggcgct caaccgggtt
    59641 catgctcaac agacgcgata atttttgctc gataatttcc cattcaggca gggggtaaag
    59701 cagcaggcac gggtgatgaa tgtcaatggt gcaaaccatt tgaccggcag cgttctcaag
    59761 cagctgttcc cgataacggg taggcactga taagcgccct ttgctgtcga gattgactaa
    59821 cgttgctccc cggaacatgc cagcctcacc ccttattccc actttgcccc acaatttccc
    59881 actgaaagga gtttacggag cggaggaaaa gcttgtcaag ccagtcacaa ggcataccga
    59941 ggtaaaaaac ccgatgttta cggctaatat cagcgttgtt tattttacgt ccaacgtgcg
    60001 aagcaaatta acgttatgaa tagctgtaag aaaaaatccc tcatcgtcat cactgtttta
    60061 aaatgactca ctattttcca cagcaaatat aaagtgtcag tttgcgacgc gagcggcatt
    60121 ttaggacata tcttccccgg ttaacagtcc ttgttacgtc tgtgtggcgc ttgatgccag
    60181 cgggtttgcg caaaagcaaa gaaaagtgtt tgttaaatcg gcaattcatc gccgcatgta
    60241 acaaaataat gcaaaaacgc gctgtcgata tcacttaatt attgtgaatt tcagctggtg
    60301 agttaattaa cgggcgggtt taaaaccatt tatcaataat agtcaggaat agtcttattt
    60361 actttaagga tattgat
//
LOCUS       DAEEUE010000011        59845 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.011, whole genome shotgun sequence.
ACCESSION   DAEEUE010000011 DAEEUE010000000
VERSION     DAEEUE010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59845
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.011"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            533..1822
                     /gene="sdaC"
                     /locus_tag="KLK84_000726"
     CDS             533..1822
                     /gene="sdaC"
                     /locus_tag="KLK84_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HBE2008180.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1880..3247
                     /gene="sdaB"
                     /locus_tag="KLK84_000727"
     CDS             1880..3247
                     /gene="sdaB"
                     /locus_tag="KLK84_000727"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HBE2008181.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSLDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3358..4113
                     /gene="xni"
                     /locus_tag="KLK84_000728"
     CDS             3358..4113
                     /gene="xni"
                     /locus_tag="KLK84_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HBE2008182.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="KLK84_000729"
     CDS             complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="KLK84_000729"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBE2008183.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="KLK84_000730"
     CDS             complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="KLK84_000730"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HBE2008184.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6536..7852
                     /gene="fucP"
                     /locus_tag="KLK84_000731"
     CDS             6536..7852
                     /gene="fucP"
                     /locus_tag="KLK84_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HBE2008185.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7885..9660
                     /gene="fucI"
                     /locus_tag="KLK84_000732"
     CDS             7885..9660
                     /gene="fucI"
                     /locus_tag="KLK84_000732"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HBE2008186.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYDEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATKWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9769..11188
                     /gene="fucK"
                     /locus_tag="KLK84_000733"
                     /pseudo
     CDS             9769..11188
                     /gene="fucK"
                     /locus_tag="KLK84_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11190..11612
                     /gene="fucU"
                     /locus_tag="KLK84_000734"
     CDS             11190..11612
                     /gene="fucU"
                     /locus_tag="KLK84_000734"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HBE2008187.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPLLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11670..12401
                     /gene="fucR"
                     /locus_tag="KLK84_000735"
     CDS             11670..12401
                     /gene="fucR"
                     /locus_tag="KLK84_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HBE2008188.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="KLK84_000736"
     CDS             complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="KLK84_000736"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HBE2008189.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QTQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13538..13933)
                     /locus_tag="KLK84_000737"
     CDS             complement(13538..13933)
                     /locus_tag="KLK84_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HBE2008190.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="KLK84_000738"
     CDS             complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="KLK84_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HBE2008191.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15220..15447)
                     /locus_tag="KLK84_000739"
     CDS             complement(15220..15447)
                     /locus_tag="KLK84_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HBE2008192.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15639..16844
                     /gene="csdA"
                     /locus_tag="KLK84_000740"
     CDS             15639..16844
                     /gene="csdA"
                     /locus_tag="KLK84_000740"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HBE2008193.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16844..17287
                     /gene="csdE"
                     /locus_tag="KLK84_000741"
     CDS             16844..17287
                     /gene="csdE"
                     /locus_tag="KLK84_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HBE2008194.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="KLK84_000742"
     CDS             complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="KLK84_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HBE2008195.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18383..19480)
                     /gene="mltA"
                     /locus_tag="KLK84_000743"
     CDS             complement(18383..19480)
                     /gene="mltA"
                     /locus_tag="KLK84_000743"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HBE2008196.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19689..19765
                     /locus_tag="KLK84_000744"
     tRNA            19689..19765
                     /locus_tag="KLK84_000744"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19723..19725,aa:Met,seq:cat)
     gene            19799..19875
                     /locus_tag="KLK84_000745"
     tRNA            19799..19875
                     /locus_tag="KLK84_000745"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19833..19835,aa:Met,seq:cat)
     gene            complement(19950..21203)
                     /gene="amiC"
                     /locus_tag="KLK84_000746"
     CDS             complement(19950..21203)
                     /gene="amiC"
                     /locus_tag="KLK84_000746"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HBE2008197.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21435..22766
                     /gene="argA"
                     /locus_tag="KLK84_000747"
     CDS             21435..22766
                     /gene="argA"
                     /locus_tag="KLK84_000747"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HBE2008198.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSLHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22828..24654)
                     /gene="recD"
                     /locus_tag="KLK84_000748"
     CDS             complement(22828..24654)
                     /gene="recD"
                     /locus_tag="KLK84_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HBE2008199.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24654..28196)
                     /gene="recB"
                     /locus_tag="KLK84_000749"
     CDS             complement(24654..28196)
                     /gene="recB"
                     /locus_tag="KLK84_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HBE2008200.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28189..31077)
                     /gene="ptrA"
                     /locus_tag="KLK84_000750"
                     /pseudo
     CDS             complement(28189..31077)
                     /gene="ptrA"
                     /locus_tag="KLK84_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
     gene            complement(31253..34621)
                     /gene="recC"
                     /locus_tag="KLK84_000751"
     CDS             complement(31253..34621)
                     /gene="recC"
                     /locus_tag="KLK84_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HBE2008201.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34634..34966)
                     /locus_tag="KLK84_000752"
     CDS             complement(34634..34966)
                     /locus_tag="KLK84_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBE2008202.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVMIVTALSGIQRTSMNSL
                     ASRNQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMT
                     RLHCPNRQ"
     gene            complement(34951..35358)
                     /locus_tag="KLK84_000753"
     CDS             complement(34951..35358)
                     /locus_tag="KLK84_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HBE2008203.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35355..35918)
                     /locus_tag="KLK84_000754"
     CDS             complement(35355..35918)
                     /locus_tag="KLK84_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HBE2008204.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35909..36379)
                     /locus_tag="KLK84_000755"
                     /pseudo
     CDS             complement(35909..36379)
                     /locus_tag="KLK84_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36563..37357)
                     /gene="thyA"
                     /locus_tag="KLK84_000756"
     CDS             complement(36563..37357)
                     /gene="thyA"
                     /locus_tag="KLK84_000756"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBE2008205.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(37364..38239)
                     /gene="lgt"
                     /locus_tag="KLK84_000757"
     CDS             complement(37364..38239)
                     /gene="lgt"
                     /locus_tag="KLK84_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBE2008206.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38390..40636)
                     /gene="ptsP"
                     /locus_tag="KLK84_000758"
     CDS             complement(38390..40636)
                     /gene="ptsP"
                     /locus_tag="KLK84_000758"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBE2008207.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGSANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40649..41179)
                     /gene="rppH"
                     /locus_tag="KLK84_000759"
     CDS             complement(40649..41179)
                     /gene="rppH"
                     /locus_tag="KLK84_000759"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HBE2008208.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            41864..42553
                     /gene="mutH"
                     /locus_tag="KLK84_000760"
     CDS             41864..42553
                     /gene="mutH"
                     /locus_tag="KLK84_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HBE2008209.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42622..43335
                     /locus_tag="KLK84_000761"
     CDS             42622..43335
                     /locus_tag="KLK84_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBE2008210.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43473..43691
                     /gene="ygdR"
                     /locus_tag="KLK84_000762"
     CDS             43473..43691
                     /gene="ygdR"
                     /locus_tag="KLK84_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HBE2008211.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43799..44839
                     /locus_tag="KLK84_000763"
     CDS             43799..44839
                     /locus_tag="KLK84_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HBE2008212.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRCQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44872..46062)
                     /gene="lplT"
                     /locus_tag="KLK84_000764"
     CDS             complement(44872..46062)
                     /gene="lplT"
                     /locus_tag="KLK84_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HBE2008213.1"
                     /translation="MSESVHINTSLWSKGMKAVIVAQFLSAFGDNALLFATLTLLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVVIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(46055..48214)
                     /gene="aas"
                     /locus_tag="KLK84_000765"
     CDS             complement(46055..48214)
                     /gene="aas"
                     /locus_tag="KLK84_000765"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HBE2008214.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48800..49831
                     /gene="galR"
                     /locus_tag="KLK84_000766"
     CDS             48800..49831
                     /gene="galR"
                     /locus_tag="KLK84_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HBE2008215.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49838..51100)
                     /gene="lysA"
                     /locus_tag="KLK84_000767"
     CDS             complement(49838..51100)
                     /gene="lysA"
                     /locus_tag="KLK84_000767"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBE2008216.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51222..52157
                     /gene="lysR"
                     /locus_tag="KLK84_000768"
     CDS             51222..52157
                     /gene="lysR"
                     /locus_tag="KLK84_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HBE2008217.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(52144..52836)
                     /gene="ygeA"
                     /locus_tag="KLK84_000769"
     CDS             complement(52144..52836)
                     /gene="ygeA"
                     /locus_tag="KLK84_000769"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBE2008218.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52965..54383)
                     /gene="araE"
                     /locus_tag="KLK84_000770"
     CDS             complement(52965..54383)
                     /gene="araE"
                     /locus_tag="KLK84_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HBE2008219.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGGLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(54698..55459)
                     /gene="kduD"
                     /locus_tag="KLK84_000771"
     CDS             complement(54698..55459)
                     /gene="kduD"
                     /locus_tag="KLK84_000771"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HBE2008220.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTAQGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYAIAVDGGWLAR"
     gene            complement(55489..56325)
                     /gene="kduI"
                     /locus_tag="KLK84_000772"
     CDS             complement(55489..56325)
                     /gene="kduI"
                     /locus_tag="KLK84_000772"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBE2008221.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(56612..57793)
                     /locus_tag="KLK84_000773"
     CDS             complement(56612..57793)
                     /locus_tag="KLK84_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBE2008222.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            58048..59277
                     /locus_tag="KLK84_000774"
                     /pseudo
     CDS             58048..59277
                     /locus_tag="KLK84_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT        14777 a        16053 c        14573 g        14442 t
ORIGIN      
        1 ttgccgggtt ttctcgtttt tggtcactta ctcatcaact catttcattt gttatatgaa
       61 tgtttcttac caccctcacg cgacaaatat catcacagtt aatatgtcat acaatttatg
      121 ttgcaacaca aacgtttccc tattttcata aaccgtattt tatcgctatg aaaaagaaat
      181 tatcgccatg attaactaaa agtattgatt ttttcagttc aacctacata tattgcgcgc
      241 cccggaagaa gtcagatgtc gtttaatggg caaatattgc ccttaaattc tcttttactt
      301 ttgatttaca gagtaaagcg ttgggataat ctatcttcca agcagattat tgtatttggg
      361 atcaagatca ctgatagata cataacttgt gtgtatcttt ccgccctcaa attattacgg
      421 cggtaaatga ttaagccatc gccgatagac agatttcatt tttacggtca ggcaccttcc
      481 ctggctgaac tggctaaaag ctgaattatt tgcattcctc caggagaaat agatggaaac
      541 gactcaaacc agcacgattg cgtcgaaaga ctctcgtagt gcctggcgca agacagacac
      601 catgtggatg ctgggccttt acggcacggc aatcggcgcg ggcgtgctgt tcctgccaat
      661 caacgccggt gttggcggta tgatcccgct gatcatcatg gctatccttg cgttcccgat
      721 gacgtttttt gctcaccgcg gcctgactcg cttcgtactg tctggtaaaa acccgggcga
      781 agacatcacc gaggttgtag aagaacactt tggtattggc gcaggtaaac tgattaccct
      841 gctctacttc ttcgctatct acccgatcct gctggtttat agcgtggcaa tcaccaatac
      901 cgttgaaagc ttcatgtctc accagctggg tatgacgcca ccgccgcgtg cgattctgtc
      961 gctgatcctg atcgtgggta tgatgaccat cgttcgcttt ggtgagcaga tgatcgttaa
     1021 agcgatgagt attctggtat tcccgtttgt tggcgtactg atgctgctgg ctctgtacct
     1081 gatcccgcag tggaacggcg cagcactgga aacgctgtct ctggacactg catctgcaac
     1141 cggaaacggt ctgtggatga ccctgtggct ggcaattccg gtaatggtgt tctcgttcaa
     1201 ccactctccg atcatctctt ctttcgccgt tgcgaagcgt gaagagtacg gcgatatggc
     1261 agaacagaaa tgctcgaaga tcctggcatt cgcacacatc atgatggtgc tgaccgtaat
     1321 gttcttcgtc ttcagctgcg tactgagcct gactccggca gacctggctg cggctaaaga
     1381 gcagaacatc tcgattctgt cttacctggc taaccacttt aacgcaccgg ttatcgcgtg
     1441 gatggctccg attatcgcga ttatcgctat caccaaatcc ttcctcggcc actacctggg
     1501 cgcacgtgaa ggcttcaacg gtatggtgat taaatctctg cgtggtaaag gtaagtctat
     1561 cgaaatcaac aagctgaacc gtatcactgc gctgttcatg ctggtaacga cctggattgt
     1621 tgccaccctg aacccgagca tcctgggtat gattgaaacc ctgggcggcc caatcatcgc
     1681 gatgatcctg ttcctgatgc cgatgtacgc aattcagaaa gtaccggcaa tgcgtaagta
     1741 cagcggtcac atcagcaacg tattcgttgt cgtgatgggt ctgattgcaa tctccgcaat
     1801 cttctactct ctgttcagct aagtcctttc gcgccgcttt cgggcggcgc ttcctccgtt
     1861 ttaacgcgat gtatttccta tgattagcgt attcgatatt ttcaaaatcg gcattggccc
     1921 ttccagttct cataccgttg gaccaatgaa agcgggtaaa caatttaccg acgatctgat
     1981 tgcccgtaac ctgcttaaag acgtgacccg cgtggtggtt gacgtgtacg gctcgctctc
     2041 tctgactggt aaaggccacc acacagatat cgccattatt atgggcctgg cgggtaacct
     2101 gccggatacc gtggatatcg attccatccc cggttttatt caggatgtga atactcatgg
     2161 tcgcctgatg ctggcaaacg gtcagcatga agtggagttc ccggttgatc agtgcatgaa
     2221 cttccatgca gataaccttt ctctgcatga aaacggtatg cgcattaccg cgctggcggg
     2281 cgataaagtc gtttacagcc agacttacta ctctattggc ggtggcttta tcgttgatga
     2341 agagcatttt ggccagcagg atagcgcacc ggttgaagtt ccttatccgt acagttcagc
     2401 agccgatctg caaaaacatt gtcaggaaac cgggctgtca ctctctggcc tgatgatgaa
     2461 aaacgagctg gcgctgcaca gtaaagaaga gctggaacag cacctggcga acgtctggga
     2521 agtcatgcgt ggcggtattg agcgcggtat ttccaccgaa ggcgtgttgc ctggcaaact
     2581 gcgcgttcca cgccgtgctg cggcactacg ccggatgctg gtcagcctgg ataaaaccac
     2641 cactgacccg atggcggttg ttgactggat caacatgttt gcactggcag tgaacgaaga
     2701 gaacgctgct ggcggtcgcg tggtgactgc gccgactaac ggtgcgtgcg ggattatccc
     2761 ggctgtgctg gcgtactacg ataagtttat ccgcgaagtg aacgctaact cactggctcg
     2821 ttacctgctg gtagccagcg ccattggttc tctttataag atgaacgcgt cgatttctgg
     2881 tgccgaagtg ggttgccagg gtgaagttgg cgtggcgtgc tctatggcgg cagctggtct
     2941 ggcagaactg ttaggcgcaa gcccggcgca ggtttgcatc gcagcggaaa tcgcgatgga
     3001 gcacaacctc ggtctgactt gtgacccggt cgcggggcag gttcaggtac cgtgcattga
     3061 gcgtaacgcc attgcagcag tgaaagcggt gaacgccgcg cgtatggcac tgcgccgtac
     3121 cagcgaaccg cgcgtctgcc tcgataaagt tatcgaaacc atgtacgaaa caggtaaaga
     3181 catgaacgcc aagtaccgcg aaacctctcg cggcggcctg gcgatgaaga tcgttgcctg
     3241 cgattaatct ctcccagagg cctcgttttg cgaagcctct tcctgttttc tcaaccagcc
     3301 atagcctgtt ccggcatcga atgttaccct tatcgcctga tctttaaggg ggttatcgtg
     3361 gctgttcatt tgcttattgt cgatgcactg aatcttatcc gtcgcattca tgccgttcag
     3421 ggttcgccct gtgtcgaaac ctgccagcat gcgctcgatc agctcattat gcacagccag
     3481 ccaacccacg cggtcgccgt ttttgatgat gaaaaccgca gtagcggctg gcgtcatcag
     3541 cgtttaccag attacaaagc ggatcgaccg ccaatgccgg aagagttgca cgacgagatg
     3601 cctgcattac gcgccgcctt tgagcaacgc ggcgtcccgt gctggtcagc cagcggcaac
     3661 gaagccgatg acttagccgc cacactggcg gtcaaagtga cacaggccgg gcatcaggca
     3721 acgattgttt cgacagataa aggctactgt cagttacttt caccgacatt acgtattcgt
     3781 gattacttcc agaaacgttg gctggatgcg ccatttatcg ataaagaatt tggcgttcaa
     3841 ccgcagcagt tgcccgatta ctggggactt gcggggatca gcagttcaaa ggtaccgggt
     3901 gttgcgggaa tcggaccaaa aagcgccacg cagctgctgg tcgagtttca gagtctggaa
     3961 gggatatatg agaatctgga tgcggttgcc gaaaagtggc gcaaaaaatt agaaacccat
     4021 aaagagatgg cgtttctgtg ccgcgatatt gcccgcttac aaaccgattt gcatatcgac
     4081 ggcaatttac agcaattgcg gttggtacgg taacggcgag ccggatacgc cgcaaatcgt
     4141 atccggcatt atcacatcag cgcatttacc aggcggtatg gtaaagctct acaatatcct
     4201 caagcgttgc ttcacgcggg ttgccaccgg tacaaacatc atccagtgcc gcctgagcca
     4261 gtgccgaaat gtcttccttg cgtacaccaa catcacgcaa atgtggcgga ataccgacat
     4321 cacggttgag ggcaaacacc gcttcaacag cggcattacg cgccccttcc aggctcatac
     4381 cttccacttt cacgcccata acgcgcgcga tatcgcggta cttctcaccg gtaaagtcag
     4441 cgttatagcg catgacatgc ggtaacagga tggcgttcgc aacaccgtgt ggagtgttat
     4501 aaaacgcgcc cagtggatgc gccataccat gcaccaaccc taacccaaca ttcgagaagc
     4561 ccatacccgc aacatactgc ccgagcgcca tttcttctcc ggcatcctta tcaccagcaa
     4621 ccgatcctcg cagcgcccca gcaatgattt caatcgcttt aatgtgcagt gcatcggtta
     4681 gtgcccacgc gccacgggta atatacccct caatagcatg agtgagcgca tcaacacccg
     4741 tcgcggcttt cagcgccgga ggcataccat ccatcatgtc agcatcaata aacgccacct
     4801 gcgggatatc atgcggatca acgcaaacaa acttgcgccg tttttcttcg tcagtgatca
     4861 cgtagttaat ggtcacttct gccgcagtac ctgctgtggt ggggattgcc agaatcggta
     4921 cactgggttt attggtcggg gaaagccctt ccaggctacg cacatcggca aactccgggt
     4981 tgttgctgat aatgccaatt gctttacaag tatcctgtgg agaaccacca ccaatagcga
     5041 tcaggtaatc cgcgccgcta ttctggaata caccgagccc ttctttgacg acagtaattg
     5101 ttgggttggg cactacgccg tcgtaaatcg cccatgccag ccctgcagca tccatcttat
     5161 cggtcacttt cgccaccacg ccgcattgca ccagcgtttt atcggtgacg atcagcgcct
     5221 tctgataacc acggcgtttc acctcatcgg ttaaagcccc aacagcaccc cgaccaaacc
     5281 atgccgtttc gttcagaatc attctgttag ccatcatcct tctccttgtt gctttacgaa
     5341 attactcttc aatccgtaac ccataggttt tgaatttctc cagcactacg gcaatctctt
     5401 catcgctcag cactggcacc gggtccgtaa tcgccagggt cgtcaggtaa agttgcgcca
     5461 gcacttcaac ttcatgcgcc agccataacg ctttttccag attcacctca caagcgataa
     5521 gcccatgatg ttgtaacaaa gttgccttac gatttttgag agccagcgca acatgttcag
     5581 aaagttcgcg tgttccaaag gtcgcataag gcgcgcaagg aatagaatta ccgccagccg
     5641 ccgcaatcat gtagtgaata gcggggatcg atcggttaag aatggaaact gccgtgcaat
     5701 gaacggcatg attgtgaaca accgcgttgg catccggtct gctttgatag gctgccatat
     5761 ggaaacgcca ttcgcttgag gggagctttc cttcctcatg tttaccgttg ccatcaataa
     5821 agacaatatg cgactccgtc agtttttcat atggaatgcc tgtaggcgta atcagcatcc
     5881 catcctgata acgtacactg acgttccccg ctgtcccctg gttcagtccc aggcgggtca
     5941 tttccaggca agtgtcaata atctgacgag caagtttatt tcgttccatt agctacctct
     6001 ctctgattca aaacagggca ataatgttgt tcctttcaca ctattgaatt agccgtttaa
     6061 ttacccacca tcttcttcct gattaacaag aaagaaattc acaagcttat attttgtgac
     6121 ctagctcaac taatcacagt aaataactgc aagttctctt tttataaccc cgttaaaaat
     6181 gaccgctctt aaaaatattt atcaaaacgg tcatttttcc atttcccctc gccagaaatg
     6241 cccgttttcg gcacaaacaa ttaatacggt catctggttt gtgtttttta tgatttattt
     6301 tctgaaacgg gcatgaaatt tcgattatta aagtgatggt agtcacataa agtcaccttc
     6361 cagctaataa gtgtgaccgc cgtcatatta cagagcgttt tttatttgaa aatgaatcca
     6421 tgagttcatt tcagacaggc aaatattcac cgatatgaag cccgaactcg ctggttttgc
     6481 acttttgaaa acataaccga ttacgtgctt aagcttctga acctaagagg atgctatggg
     6541 aaacacatca atacaaacgc agagttaccg tgcggtagat aaagatgcag ggcaaagcag
     6601 aagttacatt attccattcg cgctgctgtg ctcactgttt tttctttggg cggtagccaa
     6661 taaccttaac gacattttat tacctcaatt ccagcaggct tttacgctga caaatttcca
     6721 ggctggcctg atccaatcgg ccttttactt tggttatttc attatcccaa tccctgctgg
     6781 gatattgatg aaaaaactca gttataaagc agggattatt accggattat ttttatatgc
     6841 cttgggcgct gcattattct ggcccgctgc agaaataatg aactacacct tatttttagt
     6901 tggcctattt attattgcag ccggattagg ttgtctggaa actgccgcaa acccttttgt
     6961 tacggtatta gggccggaaa gcagtagtca cttccgctta aatcttgcgc aaacatttaa
     7021 ctcgtttggc gcaattatcg cggttgtctt tgggcaaagt cttattttgt ctaacgtgcc
     7081 acatcaatcg caagacgttc tcgataaaat gtctccagag caattgagtg cgtataaaca
     7141 cagcctggta ttatcggtac agacacctta tatgatcatc gtggctatcg tgttactggt
     7201 cgccctgctg atcatgctga cgaaattccc ggcattgcag agtgataatc acagtgacgc
     7261 caaacaagga tcgttctccg catcgctttc tcgtctggcg cgtattcgtc actggcgctg
     7321 ggcggtatta gcgcaattct gctatgtcgg cgcacaaacg gcctgctgga gctatttgat
     7381 tcgctacgct gtagaagaaa ttccaggtat gactgcaggc tttgccgcta actatttaac
     7441 cggaaccatg gtgtgcttct ttattggtcg tttcaccggt acctggctca tcagtcgctt
     7501 cgcaccacac aaagtcctgg ccgcctacgc attaatcgct atggcactgt gcctgatctc
     7561 agccttcgct ggcggtcatg tgggcttaat agccctgact ttatgcagcg cctttatgtc
     7621 gattcagtac ccaacaatct tctcgctggg cattaagaat ctcggccagg acaccaaata
     7681 cggttcgtcc ttcatcgtta tgaccattat tggcggcggt attgtcactc cggtcatggg
     7741 ttttgtcagt gacgcggcgg gcaacatccc cactgctgaa ctgatccccg cactctgctt
     7801 cgcggtcatc tttatctttg cccgtttccg ttctcaaacg gcaactaact gaacatattt
     7861 tccgaataaa gtgaggaatc tgtaatgaaa aaaatcagct taccgaaaat tggtatccgc
     7921 ccggttattg acggtcgtcg catgggtgtt cgtgagtcgc ttgaagaaca aacaatgaat
     7981 atggcgaaag ctacggccgc actgctgacc gagaaactgc gccatgcctg cggagctgcc
     8041 gtcgagtgtg tcatttccga tacctgtatc gcgggtatgg ctgaagccgc tgcttgcgaa
     8101 gaaaaattca gcagccagaa tgtaggcctc accattacgg taacgccttg ctggtgctat
     8161 ggcagtgaaa ccatcgacat ggatccaacc cgcccgaaag ccatttgggg ctttaacggc
     8221 actgaacgcc ccggcgctgt ttacctggca gcggctctgg cagctcacag ccagaaaggc
     8281 atcccggcat tctccattta cggtcatgac gttcaggatg ccgatgacac atcgattcct
     8341 gccgatgttg aagaaaaact gctgcgcttt gcccgcgccg gtttggccgt cgccagcatg
     8401 aaaggtaaaa gctatctgtc gctgggcggc gtttcgatgg gtatcgccgg ttctattgtt
     8461 gatcacaact tctttgaatc ctggctggga atgaaagtcc aggcggtgga tatgaccgaa
     8521 ctgcgtcgcc gtatcgatca gaagatttac gacgaagccg aactggaaat ggcactggcc
     8581 tgggctgata aaaacttccg ctatgacgaa gatgaaaata acaaacagta tcaacgcaat
     8641 gccgagcaaa gccgcgcagt tctgcgcgaa agtttactga tggcgatgtg tatccgcgac
     8701 atgatgcaag gcaacagcaa actggccgat attggtcgcg tggaagaatc acttggctac
     8761 aacgccatcg ctgcgggctt ccaggggcaa cgtcactgga ccgatcaata tcccaatggt
     8821 gacaccgccg aagcgatcct caacagttca tttgactgga atggcgtgcg cgaacccttt
     8881 gtcgtggcga ccgaaaacga cagtcttaac ggcgtggcaa tgctaatggg tcaccagctc
     8941 accggcaccg ctcaggtatt tgccgatgtg cgtacctact ggtcaccaga agcaattgag
     9001 cgtgtaacgg ggcataaact ggacggactg gcagaacacg gcatcatcca tttgatcaac
     9061 tcaggttctg ctgcgctgga cggttcctgt aaacaacgcg acagcgaagg taacccgacg
     9121 atgaagccgc actgggaaat ctctcagcaa gaggctgacg cttgcctcgc cgctaccaaa
     9181 tggtgcccgg cgatccacga atacttccgt ggcggcggtt actcttcccg cttccttacc
     9241 gaaggcggcg tcccgttcac catgactcgt gtcaacatca tcaaaggcct gggaccggta
     9301 ctgcaaatcg cggaaggctg gagcgtggaa ttgccgaagg atgtgcatga catcctcaac
     9361 aaacgcacca actcaacctg gccaaccacc tggtttgcac cgcgcctcac tggtaaaggg
     9421 ccgtttacgg atgtgtattc ggtaatggcg aactggggtg ccaaccacgg agttctgacc
     9481 atcggccacg ttggcgcaga ctttatcacc cttgcctcca tgctgcgtat cccggtatgt
     9541 atgcacaacg ttgaagagac caaagtgtat cgtccttctg cctgggctgc gcacggcatg
     9601 gatattgaag gccaggatta ccgcgcttgc cagaactacg gtccgttgta caagcgttaa
     9661 tacattacac cctgatgtga tgcccggtcg ctccggctac cgggcctgaa caagcaagag
     9721 tggttagccg gataaacaat gttatccggc tatattgcag gagcgattat gaaacaagaa
     9781 gttatcctgg tactcgactg tggcgcgacc aatgtcaggg ccatcgcggt taatcggcag
     9841 ggaaaaattg ttgcccgcgc ctcaacgcct aatgccagcg atatcgcgat ggaaaacaac
     9901 acctggcacc agtggtcttt agactgccat tttgcaacgc tttgctgatt gctgtcggca
     9961 aatcaatagt gaactgactg aatgccacat ccgcggtatc gccgtcacca cctttagtgt
    10021 ggatggcgct ctggtagata agcaaggcaa tctgctctat ccgatcatta gctggaaatg
    10081 tccgcgaaca gcagcggtta tggacaatat tgaacggtta atctccgcac agcggttgca
    10141 ggctatttct ggcgtcggag cctttagttt caatacgtta tataagttgg tgtggttgaa
    10201 agaaaatcat ccacaactgc tggaacgcgc gcacgcctgg ctcttcattt cgtcgctgat
    10261 taaccaccgt ttaaccagcg aattcactac tgatatcacg atggccggaa ccagccagat
    10321 gctggatatc cagcaacgcg atttcagtcc gcaaatttta caagccaccg gtattccacg
    10381 ccgactcttc cctcgtctgg tggaagcggg tgaacagatt ggtacgctac agaacaacgc
    10441 cgcagcaatg ctcggcttac ccgtcggcat accggtgatt tccgcaggtc acgataccca
    10501 gttcgccctt tttggcgctg gtgccgaaca aaatgaaccc gtgctctcct ccggtacatg
    10561 ggaaatttta atggttcgca gcgcctaggt tgatacttcg ctgttaagtc agtacgccgg
    10621 ttccacctgc gaactagata gccaggcatg gttgtataac ccaggtatgc aatggctggc
    10681 atccggcgtg ctggaatggg tgagaaaact gttctggacg gctgaaacac cctggcaaat
    10741 gttgattgaa gaagctcgtc tgatcgcacc tggcgcggat ggtgtgaaaa tgcagtgtga
    10801 tttattgtcg tgtcagaacg ctggctggca aggagtgacg cttaatacca cgcgggggca
    10861 tttctatcgc gcggcgctgg aagggttaac tgcgcaatta cagcgcaatc tacagatgct
    10921 ggaaaaaatc gggcacttta aagcctatga attattgtta gtcggtggag gaagtcgcaa
    10981 cacattgtgg aatcagatta aagccaatat gcttgatatt ccggtaaaag ttctcgacga
    11041 tgccgaaacg accgtcgcag gagctgcgct gttcggttgg tatggcgtag gggaatttaa
    11101 cagcccggaa gaagcccgtg cacagattca ttatcagtac cgttatttct acccgcaaac
    11161 tgaacctgaa tttatagagg aagtgtgaaa tgctgaaaac aatttcgccg ttaatttctc
    11221 ccgaactatt gaaagtgctg gcagagatgg gacatggaga tgaaattatt ttttccgatg
    11281 ctcactttcc cgcccattcg atggaaccgc aggtgatccg cgctgatggc ctgttggtga
    11341 gcgacttgct ccaggcgatt atcccgttat ttgagctgga cagttatgcc ccgctgctgg
    11401 tgatgatggc ggcggtagag ggtgacactc tcgatcctga agtagaacga cgttaccgta
    11461 atgcgctttc actacaagcc ccgtgtcctg acatcatccg catcaatcgt tttgcgtttt
    11521 atgaacgggc gcaaaaagcc tttgcgatcg ttatcacagg cgaacgagcg aagtacggga
    11581 atattctttt aaaaaaaggg gtaacaccgt aatctcatac cggtacgccc gcatgacgcg
    11641 ggcggttatc gaatgatggg gtgaaaaata tgaaagcggc acgccagcaa gcgatagtcg
    11701 acctgctgct gaaccatacc agcctgacca cggaagctct ctctgaacag ctaaaggtca
    11761 gtaaagaaac cattcgtcgc gatctcaatg aattacagac gcagggtaaa attctgcgca
    11821 atcatggacg cgctaaatat atccaccgtc aaaatcaaga cagtggcgat ccctttcaca
    11881 tcaggctgaa aagccattat gcgcataaag cagatatcgc gcgcgaggcg ctcgcgtgga
    11941 ttgaagaagg gatggtgata gccttagacg ccagttcaac ttgctggtat ctggcacgcc
    12001 agttgcctga catcaacatt caggtcttca ccaatagcca tccgatttgc catgaactcg
    12061 gtaaacgcga acgcattcaa ctgatcagtt ccggcggcac acttgagcgc aaatatggct
    12121 gttacgtcaa tccctcgctg atttcccaac ttaaatcgct ggaaatcgat ctgtttattt
    12181 tttcttgtga agggatcgat agcagcggcg cactgtggga ctccaatgcg atcaacgctg
    12241 attacaaatc gatgctatta aaacgtgccg cgcaatcgtt gttattgatt gataaaagta
    12301 aatttaatcg ttcaggggaa gcccgcatcg ggcatctgga tgaggtaacg cacattattt
    12361 ctgatgagcg ccaggttgca acttctttgg taacagcctg acggaaaggg tagcaggccg
    12421 gagacgacgc cccggccttg cctgttatcg ctcgtcgcga cgaccaccca ccgcagccca
    12481 gatgcggcgg acgtgcaccg tcacttcttc gcgatcgtga tacaactgcc gcgcctgagt
    12541 ctgagcattt atgccatgtt catcaagctg tgcctgaata tacgccagat tgtgtgatac
    12601 ttcttcgtag cgttttttca tcggcagttt gagattgaaa atggtttcac ggcaccagcc
    12661 attaaccagc cactgcgcca tcaatgccgc aactttagcc ggtttttcaa ccatatcgca
    12721 taccatccag gagatattgc tgcgtgtcgg acggaatttg aaaccgtctt cccgcagcca
    12781 cgtcacctgc ccggtatcca tcagactttg cgccatcggg ccgttgtcga cggaataaac
    12841 ccacatgttg cgcttcacca gttggtaggt ccagccgcca gggcaagcgc ctaaatccac
    12901 cgcccacatc ccgttcgcca ggcgttcatc ccactcatcc gcaggaataa acacatgaaa
    12961 tgcctcttcc agtttgagcg tggaacgact cggcgcctct gccggaaatt tcaggcgcgg
    13021 aatgcccata tagaacggcg aattattgtt gctgtatgag taaccggtat agcagcatcc
    13081 tggtgcaata aagaatacat gcacaaccgg acgcttcggc gtttcatagt tcgccagcac
    13141 cccagcatcg cgcaaggcag cgcgtagcgg aacagtgaac ttacggcaga atttcagcaa
    13201 ctctttgctt tcgttggtat cggcaacttc aacacgcagt tcaccgccct tctctactac
    13261 accctgtaac atgccgacaa tgggggtaat acgatcttct ggcggcaaat gctgcaggag
    13321 ttcccccacc acaaaccact ggcgggcaaa aattaatgaa ctgaacggca gctcacggat
    13381 taacttatcg ccatcatcag gttgataaca ttcataaatg acataacccg cattctcttt
    13441 cacgcgggca aaaccgaaaa tttcccgctg gccggcttta tcggtaattt ctgcggcgca
    13501 ctctttttca aagcccggac ggcacagcaa tacaacctta ttcatgactt acgcccttgc
    13561 gctttaaacg gatagcacca actaacatta acgcccagcc cgcaaggaag ctcacgccgc
    13621 caaccggtgt gacaaacgcc cacaaacgca gatgggacag cgccaggcaa taaaggctgc
    13681 cgctgaacag caccgtgcct aacgcgagga aaacgctact ccagtaaaac cagatactga
    13741 tgcgacgctg cattgccacc gccagaccta agatcgccag cgtatgaaac gcctggtatt
    13801 cgagaccggt ttggatccag cccatctcaa cggcccccat ggttttactt aacacatgcg
    13861 caccaaaagc gcccagtgcc acaaaaatga agccgctaat agcggcgaaa atcagcataa
    13921 aacggctggt catggtcgta ccctacgtaa attattgttc ataacgaaag cggaattttt
    13981 cttgttcagc agcggctttc gccaggatcc attggcgaaa ggcggctatt ttacccagtt
    14041 ctgcctggct gtcatgacaa accagataaa aagcattttt actgaccaga acatcattaa
    14101 acgggcaaac aagacgtccg gcctcgattt cagattgcgc catcacgtta tttgccagcg
    14161 ccactccctg cccgtgaata gccgcttgca gcaccatggc gctatgacta aaaattggcc
    14221 cttgctgaac gttgatatga ttaaacccca actgtcgggt atatgtctgc cagtcacggc
    14281 gtgacgcatc atgtaataac gtatgtttag ccagatcttc cggggtcttc aagggttttt
    14341 cgcctgtcag cagtagcggc gaacacaccg gcaataaata ttcggcgtac agtttttcca
    14401 cccgtagccc cggccagttg ccccgaccat aaaatatcgc cacatcaaca tcatccgcca
    14461 gcttatcttc ctgacgatca accgcctgga ttcgaacgtc aattcccgga taagctgaat
    14521 taaagctgga aagtcgcgga accaaccaat gaatggcgaa actggggagt aaactgaccg
    14581 tcaacgcccc cttggcgcta cgggcctgga gtttacgcgt cgcttcggtt aattgcgaaa
    14641 atatctcttt gatatcgagg aaatagcttt gcccttcctc ggtcagcagg agtgaacgat
    14701 tacggcggcg gaacagtttt agccccaaaa aatcctcaag agacttgatt tgatgactta
    14761 ctgcggcctg ggtcacaaaa agctcttctg ctgcgcgagt aaaacttaaa tggcgtgctg
    14821 cggcatcaaa aactcgtaag gcatttagcg gtggtaatcg tttagacatg gcttttaaac
    14881 tttgatgtta aatgaattta acaattagat cacactgtgt aacctattag tttttttaat
    14941 ctgagccatt ataaattgtc cgttgagctt ctaccagcaa atacctatag tggcggcact
    15001 tcctgagccg gaacgaaaag ttttatcgga atgcgtattc tggtgaactt ttggcttacg
    15061 gttgtgatgt tgtgttgttg tgtttgcaat tggtctgcga ttcagaccat ggtagcaaag
    15121 ctaccttttt tcacttcctg tacatttacc ctgtctgtcc atagtgatta atgtagcacc
    15181 gcctaattgc ggtgcttttt tttaccttgc gatcgcgaat tactgttcca gttcgaccat
    15241 ctctttcacg tcagtacggt tgatctgctg tttgttgcca ttagcgtctt tatacgaaat
    15301 cataccgata tcgttatcag tctgtggttt gccgtcagag acgatggtac gtccgtcatt
    15361 ggtgtgcatc acatagttcg aaccggaaca ggcgctcagg gcaaaagtca gcatacaggc
    15421 agaaataatt gcggcagtct ttttcatcat cttctcctta aaacgattaa taaatcttaa
    15481 tagtattgat taacaggcta aaattaacgc ctaacactat tcagcatatg ttacttacgc
    15541 ggcttcgcca ggatatccag ataattctga tggttagcac tctccttgta tcaaagtgaa
    15601 ttttgcgtca cgatcggtgc atcaagccga ggagtaccat gaacgttttt aatcccgcgc
    15661 agtttcgcgc ccagtttccc gcactacagg atgcgggcgt ctatctcgac agcgccgcga
    15721 ccgcgcttaa acctgaagcc gtggttgaag ccactcgaca gttttacagc ctgagcgccg
    15781 gaaacgtcca tcgcagccag tttgccgaag cccaacgcct gaccgcgcgt tatgaagctg
    15841 cacgagagaa agtggcgcaa ttactgaatg caccggatga taaaactatc gtctggacgc
    15901 gcggcaccac tgaatccatc aacatggtgg cacaatgcta tgcgcgtccg cgtctgcaac
    15961 cgggcgatga gattattgtc agcgtggcag aacaccacgc taacctcgtc ccctggctga
    16021 tggtcgccca acaaactaga gccaaagtgg tgaaattgcc gcttaatgcg cagcgactgc
    16081 cggatgtcga tttgttgcca gagctgatta ctccccgtag tcggattctg gcgttgggtc
    16141 agatgtcgaa cgtgactggc ggttgcccgg atctggcgcg agcgattacc tttgctcatt
    16201 cagccgggat ggtggtgatg gttgatggtg ctcagggggc ggtgcatttc cccgcggatg
    16261 ttcagcaact ggatatcgat ttctatgctt tctctggtca caaactgtat ggcccgacgg
    16321 gtatcggcgt gctgtatggc aaatcagaac tgctggaagc gatgtcgccc tggctgggtg
    16381 gcggcaaaat gattcacgaa gtgagttttg acggtttcac cactcaatct gcgccgtgga
    16441 aactggaagc aggaacgcca aatgtcgctg gcgtcatagg attaagcgcg gcgctggaat
    16501 ggctggcaga ttacgatatc aaccaggccg aaagctggag ccgtagctta gcaacgcttg
    16561 cagaagaagc gctggcgaaa cgtccaggct ttcgttcatt ccgctgccag gattccagcc
    16621 tgctggcctt tgattttgct ggcgttcatc acagcgatat ggtgacgcta ctggcggagt
    16681 acggtattgc cttgcgggcc gggcaacatt gcgctcagcc gctactggca gaattaggcg
    16741 taaccggcac actgcgcgcc tcatttgcgc catataatac aaagagtgat gtggatgcgc
    16801 tggtgaatgc cgttgaccgc gcgctggaat tattggtgga ttaatgacaa acccgcaatt
    16861 cgccggacat ccgttcggca caaccgtaac cgcagaaacg ttacgcaata ccttcgcacc
    16921 gttgtcgcaa tgggaagata aatatcgcca gttgatcatg ctggggaaac agcttccggc
    16981 tttgccagac gagttaaaag cgcaggctaa agagattgcc ggatgcgaaa accgcgtctg
    17041 gctgggatat accgtggctg aaaacggcaa aatgcacttc tttggcaaca gcgaagggcg
    17101 cattgtgcgc ggcctgttgg cggtgttgtt gactgccgtt gaggggaaaa ccgccgccga
    17161 attacaggca cagtcgccgc tggcattgtt tgatgagctg ggattacgtg cgcagcttag
    17221 cgcctcacgc agccaggggt taaatgcgtt aagcgaggcg attatcgctg cgacgaagca
    17281 ggtttaagca cagaaccggg tcggataaga cgttcgcgtc tcatccgacc tgattgttta
    17341 accctgacgc gccgctttcg ccatcatctt cttcagcgca tgagaaaccg caacaaaccc
    17401 aaaggtggcg gtcaccatcg ttgccgcgcc aaatccagat gcacaatcca tccgcttcgg
    17461 cccttctgcc gtggctttca tcgcacacac cgtaccgtct gactgcgggt acaccagcgc
    17521 ttcagtagaa aacacgcaat ccacgccgag cttaccttta ctgtttttca ctacgccaaa
    17581 atcgcttttc aggcgctcgc gcaacttcgc cgccagcgga tcctgaatcg ttttcgccag
    17641 atcggtaacc tgaatctgcg tcgggtcaat ctgcccaccc gcgccaccgg tcgtgaccag
    17701 cgggatttta ttgcgccgac aataagcaat cagcgccgct ttgggccgta cactatcaat
    17761 ggcatcaatc acgtacgaat aaccggcact catatattgc gctacgttat ccggcgtcac
    17821 gaaatcatcc accaccgtta cacggcactc cgggttaatc tggcgaatgc gctccgccat
    17881 gacttccgct tttgccagcc cgacgttatc gcgcagagcg tgaatttgcc gattggtatt
    17941 ggtgacgcac acatcatcca tatcgataag cgtgattgcg ccaatccccg tgcgcgccag
    18001 cgcttccgcc gcccaggaac cgacaccacc gataccaacc acacaaatat gcgcgtcagc
    18061 aaacagttgc aacgcttttt caccatacag acgcgctgtg ccaccaaaac gctgacgcca
    18121 tgcatcacta attaccacag acatagcacc tcagaattaa aaagggcgag gttatccctc
    18181 accctaaacg cataaatgcc tgatgcgcta cgcttatcag gcctacataa ctcctgcaat
    18241 attttggatt tgcatgattt gtaggccgga taaggcgttc acgccgcatc cggtatgaac
    18301 aacgcgcact tagtcaataa tcaggatgtg aggttacccc tcaccctgtc atatccgtaa
    18361 aaacggcata cagaatatca catcagccgc taaagacgtt acctgcgccc ggagccgttt
    18421 tcagcaccca aacccgtcca tagtggttgt accaacctgc gcggtgtccg gcttccggcc
    18481 cgatcccttg atagatatcg aagtgttggc ctttgattgc gcccccgaca tccaacgcca
    18541 ccatcagacg caattcgtac tgaccgttaa atttgccgtt attatccagc agcggcactt
    18601 ctgccagcaa ggtagtacct ggcggaataa ttgaacgatc ggaggcaaca gaagcacggc
    18661 caaccagcgg caccgcactt gcccctttca ccggtgcaaa agattgcggt ttaaagaaga
    18721 cgaaagacgg gttctgttcc agcagctcgc gaacctcggc ttcactgtgt gtttcgcccc
    18781 aatgacgaat cgcctgcatc gacatatctt cttttttcac ttcgccacgg tcgatcagca
    18841 ccttaccaat gctgcgatag gcatgaccgt ttttccctgc atagctgaaa aagttaagcg
    18901 gactgccatc accaaagtcg atatacccac taccctgcac atccataatg aagttatcca
    18961 tcagagagtt gctgtaagcg aggatgtatt tatcgcccaa tgccccggcg tagatctccg
    19021 cacgagacgg cagacgacca cgttttggcg gcatacggta aataggatac tggaactcgc
    19081 cctggcgggt atggcgcgcc tgaattaccg gcgtgtaata gcctgtaaac tgcacgttac
    19141 catagttgtc ggcaccttcc atctgccagg catcaatgcc gaactggcgc atattgcggg
    19201 tatcaccgcc tgcgcgcagc cactcttgca ccgcgttata aacattactc tggttgccat
    19261 acagacgcgg tgacgaatta cggatatggt taatttgctc ggcaaaatca ccggcgttaa
    19321 tcggcgcgcc aacggcatct ggctggttca ccagagagaa aggctgggta aatttcccgt
    19381 ctttatattg ctgtccgcga tcggttggtt tggaagagca ggcggcaagc attgccacaa
    19441 ccgtgcccat aagaaggtac tttacccaac gtcctttcat tgttctcttc ttcaggttaa
    19501 aaaataaggc gcaacgaaga taacaaaccg ccgcgtttaa tgaaatgagc agcctctccc
    19561 tgacgcaaat tttgcacaaa aaataggctt tagtggtttg tttttgttca aaatcatgct
    19621 aaatccgtga tcggggtaaa aaaaaggttg catgaaaacg cgagcggagt atagtgcgca
    19681 tccacggacg cggggtggag cagcctggta gctcgtcggg ctcataaccc gaaggtcgtc
    19741 ggttcaaatc cggcccccgc aaccaatcaa atttgatgaa gtaaaagcag tacggtgacg
    19801 cggggtggag cagcttggta gctcgtcggg ctcataaccc gaaggtcgtc ggttcaaatc
    19861 cggcccccgc aaccaattat tgaacaccct aacgggtgtt tttttgtttc tgatctccca
    19921 taaaaaaagc gccattcagc gcctttttat catccccttc tcgccagcgt tgccccatcg
    19981 gcaaaatacg ctttaatccc cgcaagaata gactccgcaa cttcctgctg gaaagtcgcc
    20041 gttttcagtt tacgctcttc ctcaacgtta ctgataaacg ccgtttcgac cagaatggag
    20101 ggaatatctg gtgcctttag tacggcaaac ccggcctgtt caacttgatt tttatgcagc
    20161 ttgttgattt ttccgagctt attcagtacc gctttaccaa acttaaggct gtcagcaata
    20221 gtcagcgact gcaccatatc gaacatggtg tggtcgacat agcggtcacc gcttttgctc
    20281 acgccaccaa tcaagtccga ggcgttctgg gtttgtgcca gatattttgc cgcagtactg
    20341 gttgcgcctt tggttgagag cgcaaacacc gaggaaccgc tcggctgacg actggtaaag
    20401 gcgtcggcat ggatagagac aaacaggtca gcacgctgtt tctgggcttt tgctacgcgc
    20461 acttgcaacg gaatgaagat gtcttcattg cgcgtcatgt acaccttcat attgccctct
    20521 tcctcgatca gagagcgcaa acggcgagct atttgcaata ccacatcttt ttcgcgcgtt
    20581 ttgtatttcc ccaccgcacc ggagtcttcg ccaccgtggc cagggtcaag cataatgaca
    20641 atcggacgat cgcgccctgc tttacccggt tgtggaccac tttgcgctgg cggcacctgc
    20701 ttttcgaggt cgcctttgtt gtaatcctcc agcagcgcca gcagcgggtc ctgcatatcc
    20761 tgtgcattgg ccggatagag gtccatcacc agacgctctt taaacccggc gaccggcgca
    20821 agggcaaaca gctgcggttt tacgttttgc tttaattcaa aaaccatgcg tacggtttgc
    20881 gggtcaaatt gcccgacgcg cgccgacttg atgaacgggt cgtctgcacg aatttgtgcc
    20941 gccatcccct tgagtaccga gttcaggttt acatcttcga tatccaccac cacgcgttca
    21001 gggttactca acgcgaactg cttatatttc agctgacgat ttgattctac cgtcacgcgg
    21061 gtgtagctgg acgcaggcca gacgcgcacc gccacgacct ggctaaccgc agccaggctg
    21121 acctgactta cgctcaatag ccacatagcg cccgcgcctt gcagtaaacg acgacggctg
    21181 attgcagtgt tggatcctga catgcctctc ccgagcaaaa gaaatctaag ctgtgtaaca
    21241 agtaaacgac taatttgacc ggaaacttta gcgaaagacg cataatctgt catctaataa
    21301 acggtaaaca ttctttttat attcacggca ttactgataa aaaagtcgct ctcgcataaa
    21361 atttacactt gcaccctgcg aaaaaacaga ataaaaatac aataatttca aataatcatg
    21421 caaaggggtg tgccgtggta aaggaacgta aaaccgagct ggtcgaggga ttccgccatt
    21481 cggttcccta tatcaatacc caccggggaa aaacgtttgt catcatgctc ggcggtgaag
    21541 ccattgagca tgagaatttc tccagtatcg ttaatgatat cgggttgttg cacagcctcg
    21601 gcatccgtct ggtggtggtc tatggcgcac gtccgcagat cgacgcaaat ctggctgcgc
    21661 atcaccacga accgctgtat cacaagaata tacgtgtgac cgacgccaaa acactggaac
    21721 tggtgaagca ggctgcggga acattgcaac tggatattac tgctcgcctg tcaatgagtc
    21781 tcaataacac gccgctgcag ggcgcgcata tcaacgtcgt tagtggcaat tttattattg
    21841 cccagccgct gggcgtcgat gacggcgtgg attactgcca tagcgggcgt atccggcgga
    21901 ttgatgaaga cgcgctccat cgtcaactgg aaagcggtgc aatagtgcta atggggccgg
    21961 tcgctgtttc agtcactggc gagagcttta atctgacctc ggaagagatt gccactcaac
    22021 tagccatcaa actgaaagct gaaaagatga ttggtttttg ctcttcccag ggcgttacta
    22081 atgacgacgg tgatattgtc tccgaacttt tccctaacga agcgcaagca cgggtagaag
    22141 cccaggaaga gaaaggcgat tacaactccg gtacggtgcg ctttttgcgt ggcgcagtga
    22201 aagcctgccg cagcggcgtg cgccgctgtc atttaatcag ttatcaggaa gatggcgcgc
    22261 tgttgcaaga gttgttctca cgcgacggta tcggtacgca gattgtgatg gaaagcgccg
    22321 agcagattcg tcgcgcaaca atcaacgata ttggcggtat tctggagttg attcgcccgc
    22381 tggagcagca aggtattctg gtgcgccgtt ctcgcgagca gctggagatg gaaatcgaca
    22441 aattcaccat tattcagcgc gataacacga ctattgcctg cgccgcgctc tatccgttcc
    22501 cggaagagaa gattggggaa atggcctgtg tggcagttca cccggattac cgcagttcat
    22561 cacggggcga ggttctgctg gaacgcattg ccgctcaggc gaagcagagc ggcttaagca
    22621 aattgtttgt gctgaccacg cgcagtcttc actggttcca ggaacgtgga tttaccccgg
    22681 tggatattga tttactgccc gagagcaaaa agcagttgta caactaccag cgtaaatcca
    22741 aagtgttgat ggcggattta gggtaagcga tgaaaatcac cggatgcggc aggcgtaaca
    22801 ctcgtgcgtc gcatccggca attacgttta ttcccgcgaa ctaaacaacg ccgccagacc
    22861 actgcgccgc tcagtacgag tggcgattgc cgcacttaat atacgctcat cggcatacag
    22921 cgacagacga cgacgcgcgc gggtcaccgc ggtgtatacc agctctcgcg ttactaccgg
    22981 cgtgcgttgg ctcggtaaaa tcaacgccgc atggtcgaac tccgatccct gcgatttatg
    23041 taccgtcatc gcccacgtag tttcgtgctc tggcaagcga ctcggttgca cagacttaat
    23101 attgccgtcc ggcatcgcaa accagacgcg cgtcccctgc ccgcgatcca gcgcaatacc
    23161 gatatcgcca ttaaacaacc caagcgcgct gtcattacgg gcaatcatca ccggtcggcc
    23221 ttcgtaccaa cgagagtgcg gatgacgatg aattttgcgc ttctgttgca taaactgttc
    23281 aattcgctca ttcagtccag ccacgccaaa cggcccttcc cgcagggcgc acaaaagctg
    23341 gtactcattg aacgcctgaa tgattaaatc cggctcggca cgcgcttgca gcagatccag
    23401 ataacgtccg taacccgcaa gagcttcctc aagcatcgca atataatctt cgccgctctg
    23461 taaaagtcgt ttttcgatat cagtaaaatc ctgctgaaaa acggttttca ctgccgtttt
    23521 atcaccacgg ttgatcgccg cagctaactg accaatgcca gaatcgctgc cgaaacgata
    23581 gcttttttgc agcaggcaga gactgtcgcg caaagatgcc gcttctgtgc cggttcctgc
    23641 cggaacgtga gtccccgtca ggcggcttag ctgcctggca cgctcggcgg taaagcccgc
    23701 gttggcataa gcgcagatat cgcccagcac agccccagcc tcaaccgagg ccagttgatc
    23761 acgatcgccg agaaagatca ctcgcgcatg atctggcaag gcgtcgatca gtctcgacat
    23821 cataggcaga tcgatcattg acgcttcatc taccaccagc acatcaagat gcagcgggtt
    23881 accggcatga tgacgtaaac gctggctacc cggctgcgcg cccagcaatc ggtgcaaagt
    23941 gctggcatct tccggaatgc gtttcttttg ttcatcggtc agcggtaact gtcgcaaagc
    24001 cttgccgaga gattcggtta agcgcgcggc agctttaccc gttggtgcag ccagacggat
    24061 acggcagcgt tcgccgtcgg ccatttgaat taacgctgcc agcaacttcg ccacggtggt
    24121 cgttttaccg gtgcctgggc cgccggaaat caccgagatc cgccgcgtca gcgccactgc
    24181 cgctgcaact ttttgccagt taatttcatc gcttactgga aaaagtttgt ccagggtttg
    24241 cgccagtaga gcttcatcaa cctcaatggt atgattcacc tcattgaaga agcgcgctac
    24301 cgtccgctcg ttacaccaca tgcgattcaa ataaagacga tcgccacaga ggatcatcgg
    24361 cgtgggttca tctcccctgc tgaccgcttg agaagccagc aagcattctt cccaattttg
    24421 tagctcaccg atttcactga cacaggtcgc caacagcgga ttcgacgcct cgttattttc
    24481 cagtcgtgaa agcggcaaac agacgtgtcc ctctccggca tcatgactta acagtgccgc
    24541 cgcgagggtg acggcaggat gttcatctcc cgccacggtc agggcaaatt gcacatccag
    24601 cgggcgtagc tgtttgtgct ccacagcttc cagtaattgc ttttgcaatt tcattacgcc
    24661 tcctccaggg tcataccggc aaacatctca tccatcaggg caatcaaccc ggcgttgggt
    24721 cgggttgtgt aaatcccttg ttgcggatgt tctttatcaa cgccacgcag gaacagatag
    24781 ataacgccgc caaagtgaag atcatagtcg taatcagcaa tgcgatggcg cagataacga
    24841 tgcagcgcca gggtataaag ctgatattgc agatcatagc ggtgtgcctg catcgctgct
    24901 gccatagcct gttgggtgta agccgaactg tcttcaccca gccagttgga tttatagtcg
    24961 agcaggtaat aacgcccttc gtggcggaat accaggtcga taaagccttt taacatgccg
    25021 cgtacctgca tgaactccag cggcgggcag ccagcggata gcgggtcaaa ctggcgaatt
    25081 agtgcatcaa gctggctggc gatgagcggt tcgctaatcg gcaggtaaaa ctccatctcc
    25141 acctgtttat cgcggtcgga aagctgactc aggctaacac ccgtttcatt gagaggtgcc
    25201 tggaggacag ccgtgatcca ctcggtcaac accggttccc actgcgattc aaagccgccg
    25261 agttccagtt tttcctgtac ccagttcggg tcaaccggct gggtaaaatc gaggtcttca
    25321 aacaaactgt gcaagaatgt ccccggtgac gcaccgcgcg ggaactgatg cggtgttaac
    25381 gtcggttctt caacgacgct gaccacgcct gcggcatcaa catccagccg tggcatcaga
    25441 tcctgagcga taccgtgacc acgctgctgc aagccggagt agctggtgac acgccagtta
    25501 tcgccgggca atcgttgtag tgtcctcgca ttcagttctg cagttaaagc atcattaacc
    25561 tgccagggct ggttatcacc agtttgtgcc gtttgccagg caatatcatc atcgcataac
    25621 gcttcaatac aggtgcgaag ccctgccgca tcttgcggtt cccctttttg cagcaaacgc
    25681 ccgagcgcac tttggtggac gtcggtgtca ccttttttat cgccacgacg gcgcaccagc
    25741 ggtgcaacgc cgagactgca atgccaaacc gaacgcgtca gcgccacgta aagcaaacgc
    25801 agatcttccg ccagacgttc gacctccgcg aggtcgacgc tttctggcgc agcattaaga
    25861 tccaaaactg cctcaaacga gtggcgatcg tgataaaacg cctgatcctg gacgcggaaa
    25921 ttggtaataa atggcaacca gaccagcgga tattccagcc ctttcgattt atggatcgtg
    25981 acaatctgca ccagatgttt atcactttcg aggcgcattt gttggctgga ggcattactg
    26041 tctggctcga ggatatgttg cgataaccag cgtaccagcg catgttcact ttccagctgc
    26101 gttccggctt cttgaagcag ttcgctgata tgcaagatat cggtaagacg ccgctcaccg
    26161 cctgccgttg ccagcaagtt ttctgcaatg ttacgcgccg acatcagcgc ccgcagcatc
    26221 ggcataacgc cacgtttgcg ccagatttgc cgataaccat cgaactcttc gactaccgca
    26281 tcccacgcat gttcgtcatt attcagcgtt tcaatatcca gcgcattcag ccccatcatt
    26341 gacgttgcca gcgcactgcg cagggtgttc tcacgttcgg gcgtcatcac cgcctgcaac
    26401 aaccaaagca tttcctgcgc ttccagagtt tcaaaaacac tgtcgcggtt cgaaaggtaa
    26461 acggaaggga tttccagcaa cgttaaggca tcgcgcacct gggcggcctc ctggcggctg
    26521 cgcaccagca cgctgatgtc cgaagcacgc accggacgcg cgtcgtcgcc gttcatcagc
    26581 aacgcttcgc cccgctgtcc ggcttgtagc cagtcgcgga tttgcgcagc acatacctgc
    26641 gccatggtac tttgatagtc gccaacgccg cagctttcgc cttccatcag ccacattttc
    26701 atcgcaggct gcgtttcacc tttaaataca aaacgtaacg cctgattttt cccggctgat
    26761 ttcactggaa taaacggtat ttcgcgaaac atgaacgtgt catcagtctg gctgaaaagc
    26821 ttgttcacac tgttcaccat tccaggtgcg gagcgccagt tggtgtctaa agtgtagtgg
    26881 gcgtgaactt cactacgcgc cttcatataa gtgaagatat ccgcaccccg gaatgcatat
    26941 atggcctgct tcgggtcgcc aatcagcaac aatgcggttt ccggctgatg gtgccagata
    27001 cggcgaaaaa ttcggtactg ctgggggtcg gtatcctgaa attcatcgat cattgccacc
    27061 gggaatcgcg tacggatcgc cgctgccaac acctcgccgc tttcgctacg cagcgcggaa
    27121 tcgagccgac ttaacatgtc atcaaaaccc aattcgccac ggcggcgttt ttcacgcgct
    27181 actgtttcgc ggatctcagc caatgcgcgg gtgatcaaca gatcgcggat cgacaatggt
    27241 tctgcaagca gttgatcgat cgcctcaaac agtggatgtc gcggggtttc ccccccggcc
    27301 ttcgtgcgat cttctaagaa acgctgggag aatttttcca gcgactccgg caactgataa
    27361 ctgtttgtct cttcttctgc ccaggcgctg atcttttcga tccatttagc ctgattgcta
    27421 cggttaaact tgcgtcgatc aataccagaa gattcgatca gcgcatccag ttcacccact
    27481 gcgtcgcgcc actgctgttt taccgtatca atacgcgcca caatttgcgc gtggcgggaa
    27541 gccagcgttt catcatcggg cggcggtgct ttgataaccg gcggttcgcc ttgcagataa
    27601 cgattaatat cgcgcagcaa cgcctgcggc cctttccagg tttcaaagac gacctgggct
    27661 atttcacgcg gcagcgggta gcagtggcga cgccagaaat cggcgcaggc ctggtagcgt
    27721 agcagagact catcttcaat cagctgctgc tcaaacagca tgccggattc aaaggcattc
    27781 aggttgagca tgcgctggca aaagccgtga atagtaaaga ctgccgcttc atccatctgc
    27841 cgttcggcta acaacaacca ctgcgcggct tgcgctttat cgtcgatctc ttccagcagg
    27901 cgttcgtaca gtggattgtc ggtggtttca cgcagacagg cgatgcgcaa ctcgtggata
    27961 ttgctacgga tacgaccgcg caattctgcc gtggcagcct cggtaaaggt gaccaccagc
    28021 agttcttcaa cgatcagcgg gcggggaaag gcggcggaac cgcctagtcc aagtaacagg
    28081 cgcaaataga gcgccgcaat cgtaaaggtt ttgcctgtgc cggcagaggc ttcaatcagg
    28141 cgctcgccct gtaagggcaa gcgcaaagga tctagtgtct cggcgacatc actcattctt
    28201 ttcactcatc aggggcattg tttgctgcaa cgcgctgacg ttctcccaca ctttccagcc
    28261 ttcaggatgc acatattcgg ctttcccgtt ctggctgccg gaaatctgcg acagaatagc
    28321 catgccttgc ggctcgacca ccgcctgatg gaagaaatca gcaagttttt gcggcgtcag
    28381 cagttttatc tgggccacga ttttatcacg cgaatcgaag cgcatattgc cgcgatcgaa
    28441 atctttactt aacttcgatg cttcttcgcc gagcgtttgc ggtgcctgca gcatctgggt
    28501 aattaccgcc tgctggattt gcgcaaactc atctggcttc atcgctcgca attttgcctc
    28561 tgcggttggg aaaaacgcct tgtaacgctc ccacaagaat gaaggctgtt tatcattgct
    28621 ttgcaaaagg aagcccatgc cccactgacg ccccacgctc attggaaacg caaacacggc
    28681 atagcccaat tgttcttcgg tacgcaactg attgtagaac cacggctgta cgatctgccc
    28741 caacagagag ctataggctg agctggtgta ttcatcgtag ccagtcggta caaataccgc
    28801 tgccagtgcg gagtcggtgc tgttaccggc tttttcaaag atgacggatt gttttttatc
    28861 gaccactaca tctttgtttc gacaccactc tgaaccatca gcgcccaact gtttttgcac
    28921 atcgcgtgcc agcgttgttg cctgggcctc ggtcatgttg ccgataacca taaactctgg
    28981 tcgagcccct gattttaagg cgtcgcgata ggccagcacc tctttcaacg taatggaggg
    29041 caaaatttta cgccgttcat ctcgcgagaa gtacggcact tgcgagagca tctgcgcggg
    29101 cataatcgcc tgctcaaacg ctttaccctt ttctgcggaa tccatcatct ggttatacca
    29161 ggacttcgcc tgctcaagct gctcttccgt agcggtatag ctaaagtacc cctcgagcaa
    29221 tgcctggaac agctgcggca gacgctgggt gtaaccatta gcattaatca taaggccgtt
    29281 gttagcgttg gtggaaaaac ttatgccacc aaccgacgcc tagttgctta actgatcaag
    29341 cgccagccct gcgagataat cattgagcgc aaacatcacc tgattgcggg cgctgtccat
    29401 ggctttcgga ttacgcaaaa tcaggctgac atcagctttg ggctcgctgg caaaataacg
    29461 gcttggcgca tacaccacgc gcagattcga ctcatcaaca atcagctctg gatggtcgta
    29521 tttcttctct gacttaatca gcgagaaatc atcaggaata taagggttaa gctctggcaa
    29581 agagagcgca atgtcggcgg cttttttctg ccagtcggcg aaagtttgtg cgctgatttt
    29641 atcgacctga tacggcgcat cgacaaagta agccgttttg ttgtgcggct ctttcgggct
    29701 gatataccag atacgcgcat tctgcggcgt catcatcgcc agacgttcct ttactgcttt
    29761 agcatcgtac cgatcggcaa tattgactgc atccagcgta tgctcaacag gaacgcgaat
    29821 catggtatct gccagccatt cgacgtaatc catatcacgg gtgatcgacg gataacggaa
    29881 gtcgatatcc agcacattcg ccagttcatc gaagtattgt ttatcaatgc ctttttcacg
    29941 taacagattg agatagctaa aaattgccgc cacaacctga tcgcgattag ccaagccttt
    30001 atcagttaaa gacgcagaga tcgctaatac gccgctgttg ccgttgacga taggatcgga
    30061 gttggcgcta atgccctcaa ctaatccctg cttttgcagc cagtcagaaa gtgtacctgg
    30121 gctgcgattg ccaatcagat aggtaatcaa ttcatcggtt ttactacgga acttcgctga
    30181 gttgttatcg atgcgaaact caacgcgcaa cactttacgc ggcagcgcag ggacgtaatg
    30241 aatgataatg cccttttgcg cgtcggtgac taccggcacg gtgatttccg gttttttgct
    30301 ctctttgttc ggcacgcgac caaaggtgtc cgccgccatt tttgccaact ccggcagcgg
    30361 tttattactg taaataaccg ccttcatcaa attggcggaa tagtacttct cgtggaaatc
    30421 tttcagcgcc tgctgcaccg gattaccagg tttgtcgctt aaagtttcga ggttaccacc
    30481 agaaaacttt gaaccggggt gtgccgggtt aatggtttct gcgctgacct gtgccatgcg
    30541 catcccgtca cgcgtacgcg ccatggttaa ttcagcgttc actgcattac gctcacgttc
    30601 ggcgtatttc ttgtcgagca agggttctgc aatagcatcg gccaggcggt ctaccgcacc
    30661 gggcaaggcg tcgttctcaa cttccagata gaaagccgtg cgatacggcg cagtgctggc
    30721 attgtgacta ccgccgtgca ttttgagata ttcggccaga ctgtcagcct gcgggtactt
    30781 tttcgacccc atcagactca tatgttcaag gtaatgtgcc agcccctggt acgcctcggg
    30841 atcttccagc gacccaacgg gcactaccag cgccgagagc gatttaactg cctgcggatc
    30901 agaaaccagc aagaccacca taccgttatc cagacgtata gcctgatact ggcggttatc
    30961 tttatcactt ttacggatgg tttcctgaat cggctgccat cccgtttctg cctgacttaa
    31021 tggtgcccag agggcaacaa acaacaataa tgctttgaac caggtgctgc ggggcattca
    31081 cggacctcat aatcaacttt attttctgga cagattcaac aggttaacgc aaatcatctg
    31141 ccagaattta atcttgtgct gcacgagtca gcctatgttt atataaccat cagtccgtga
    31201 ctggtgcgca tcataaagta agcggataga ttgcgcaatt tttatacaac actcatgact
    31261 gattaaagcg aaacagcggt aacaggaaac gttgcgactg ttcaacgatg gcctccattg
    31321 tctctggtgt taattgccgc cagagccgtt gataccagat atcatcacct tcgccacgca
    31381 ccatcatgtt gccttcgtaa gcctgaagga atttcgtacg ggctttttgc aacgtggaat
    31441 cgtcatccag catggcatcg ttttgcgcgt cataacaggt ttttagccac gcgccgccac
    31501 tttcaggtaa caccagcaat ggcgcggaca ttccttcacg atacccctca atcagttgtg
    31561 agaggtaatg caaagcctgt tcggctgcaa gcggcggaaa acgccactca ccgtctttgc
    31621 gtagaaaaag gcgactttcg ccattaccac cgctggcaca gtagacaagg tgttccagcc
    31681 aaagttgcat tccctgcgcc acacttaata aagagggacg ccagcgcaac aggccatccg
    31741 gctgcacctg cggcaaccag ccagttatct gcacaccgtt gcaggcgaga tcaatttcca
    31801 tactctgccc cggctggcga caggcaatga ctctgtcggc aagctgctgc atctcctggc
    31861 actgtgtttc ccagaaaatt tcaccaaaag cgccatacgg taaatcccct gccgcccgga
    31921 agcggcggaa caagcgttcg gcatcatcct gctcaaccag tgcattcaat aactgctgat
    31981 tgatttgata acggctaagt ccttccagaa taaatggctc ggtgtctggg atttcgctgt
    32041 cttcagtatg aaagttcacc tgcaaacgca tctggaagaa cgcccgcacc ggatgtgccc
    32101 agaatcgttg tagcgtttcc agcggcacgg tttccggtaa ggtaaacggc agcggctgaa
    32161 caaattcaga atgtgcttta ccagcctggc tggccgcagg tagccattca cgagcatagc
    32221 tttgtcgttc gcctggctgg tagttttgcg gatcaaacgg catccgggta tggaggcaag
    32281 taagatgcgc ttttaccctt gcctcgcttt catcacagtt gagcgcttca tcgcccggta
    32341 gataatgact ttgcccgatg tagtcgatca gttcctgcac cagtaccgac gggaaacgtt
    32401 cactgttatc ctgaatggaa cgcccgatat agctgatata gagtttttgc tgcgcggaaa
    32461 ttaacgcttc caggaacagg tagcggtcgt catcgcgacg gctacggtcg ccacgcttcg
    32521 gtttctggct catcaggtca aagcccaatg gcgcaagctg acgtggataa acgccgtcgt
    32581 tcattcccag caggcaaacc actttgaacg gaattgaacg cattggcatc agagtacaaa
    32641 tgttaaccgg tccggcgaga aaacgctggc tgatacgttc ttgatccaga cgctgtgcca
    32701 gttcatcacg caatagtgac agcggcaccg cgtcgccata ctgcgcacct aaaccttcgg
    32761 cgataatcgc ctgccattgt tgttcgatca gcgtcatcgc cgcttcggtt tccgcatccg
    32821 gcaggaagaa ggcgttgagc atatcgcgac aaaccggcaa ccactcttct agcggacgct
    32881 cctgtgccag cccgcgacgc cagatgttta gctgcattag cagtgaagcc agatgcccca
    32941 ccagttctgc aattaagccg ctcgattcat cataaggtag aaccgattgc cactcgccct
    33001 gcgcgctctc catcgcgtag cccagcaaca tacgcgtcag gccaaatcgc caggtgtgtt
    33061 gtccggtggc ggggagttcc agctcgcgaa cgttgtcgtc atctatcccc caacgaatgc
    33121 cggattcgtt gacccactgg cgtaaataac gcagcccttc ttcggtgatg tcaaaccgcg
    33181 ccgccagcac cggcacatcc agcagcgcca gcacatcctc tgacacaaag cgactgtcag
    33241 gcagtgataa caggctgata aacgcttcca gtaccggatg tgactgccgc gcacgacggt
    33301 cggaaatggc gtaaggcagg taacgatccg caggtgcgct accaaacact gcctgaataa
    33361 acggactgta gctgtcgata tcagccacca tcacgatgat gtcgcgcgga gtaagtgtcg
    33421 gggcttcctc cagcatcgcc agcaagcgat cgtgtaaaac ttcaacttca cgctgcgggc
    33481 tatggcaaac gtggaatgtg atactgctat ccagtggatc aagcgggcgt ttgttatcgc
    33541 tacgggaaaa ctcttcgatg ttcacacccg caacggcgcg gttttccagt tccagaatgt
    33601 cagactgaat gttatgcagc aggttatctg gcgtcacatc gacaaaagcg tccagctcct
    33661 ggctgctctc caggtcagaa aggagataaa tgtagtcgcg cccaagcttc ccccatgagg
    33721 ccagcagcga gttgccgaca tcctgttcac catcgctgtt aaagagctgt ccggcatttt
    33781 cgctatcgcg aaatagcggc aattcgcgat cttcaaaact gtgtcgacgc tgacgagtca
    33841 gcagtttcgc cagataagca ggatctttaa tatcgcccca gtaataacgg caggggttgg
    33901 taaacaggag atggatttca atatgtttac ccagcgcctg gagcgcctgg agataaacag
    33961 gcggtaacgc ggaaataccg catataaaga cgcgcgaagg taaccccggc gggcaggtcg
    34021 tcgcggactc cagcgtttcg ataaagcgct gatagagatt cgcgcggtgc cagcgcggtt
    34081 gtccgagttc atgggtatat tccaccagcg ccttccacaa cggggcttgc caggcctgtg
    34141 cttctcccag cccttcaacc aaatgtcctg tttcccactg tgccagccag tccggacgat
    34201 agaccagata ctggtcaaac aggtccgccg cttttgagga aagctggaac agttttcgct
    34261 tgtcgctatc gtcagtcaga taatgccgca acagggtaaa gtcttcgcgc tccagcaatt
    34321 gcggcagcag agtcatcagt ttccagctca tgctctgttt gttaaaggcg ctctctttgg
    34381 ggatttccgg taacacccgg acgaacatat cccagataaa gctcgctggc agcggaaaat
    34441 caatgtttgc cgcaatacca aacttttgcg acagggtcat ttgcagccac tgcgccatac
    34501 cggtactttg caccagaatc atctctggtt cgaaaggatc gtccagccgt tcgcgttcga
    34561 caataaactc catcaacgct tccagcacgt ccagacgatt ggaatggtag acccttaaca
    34621 tagcggctcc tgactactga cgattcgggc aatgcaggcg ggtcatctcg ccttctctgc
    34681 ccccgggtga aactagcgta acgctgatgc tgacacatcc cgcctgcgat gtctgcattc
    34741 ggttgacctg ccagttggca ggtggcgaaa tcgcgcgcag ttgcgtttgc tgccaaccat
    34801 gccgccagag ctgttggtac tggtttctgc tggcgagact gttcattgat gttcgctgga
    34861 tacccgataa cgcagtgaca atcatcacca tcagcaccat cagcaccatc gcaaccatta
    34921 cctccggcag gctaaagcct tgttgattct tcagggaagc tgacataatg ccccctcttt
    34981 cagcggacaa aaatcgctcc agccgcgtgg cgaaaaaaca atttttccat caatgacttc
    35041 gcctgttcgc cacaacgaaa cgccttcata accggcaatc aataaggctt cattatctgc
    35101 cagtaaacgc aaacaaacct gggcatcggt tccagcgtac tgcgagcact gaactgctgg
    35161 ctgcgtctgc caggagtgca tttttcccca cgccagcgcc gactgaacga tggcctggcg
    35221 gcgcaatgac tgactttcca tgctcacgcg agaggcaaaa ctgcgatcct gctgactcat
    35281 tccttgcaat agcaagctac ccaaaaccag cagcatcagg accagagcca gtgacgaaac
    35341 acctttttcg cggttcacag gttgaatcct gtcacgctat agctggcatc caccacggtt
    35401 tgcggttcag acttactggc agcacgcata ttaaccgtca acaccggcga gaagccgctg
    35461 acatcctgac gtacgacctg aaaagtgtcg ataatgatgg catccggatt agtgacttta
    35521 tcccagccct taccttcaca ggatgtcgca ccgcgtagcg tttccagcac atgctccttc
    35581 agacgaaatc caatctggtc ggactctttt accggttcgc gatcccagat accgttactg
    35641 ttcgcatccc actgcacaat gatacagtca ccctgtccga caatttccag cccttcgccg
    35701 gtacagctgc catgacaata acccgccctc tggagatgct tcgcgacggt aaatacccgc
    35761 agccagattt catcttccag tgccagctta cgagtgctcg ttaaactttc acgctgtaac
    35821 gcaggcagaa agcgtgcagc ccccagcaac aatacgctac tgatcgccat agcaatcaac
    35881 acttccagca gagaaaaacc ttgctctttt acaggcatcc ttctgtttct ccttgctgat
    35941 aaagccggag tcttccccac ggcgaaacca ccagccacca ctcgcccgtt gagtttttga
    36001 agcgaatatg cccggcccat gcggtattac gcaggccaaa gaaagcaagc gaaggtgtca
    36061 ggtcgctcat ttcgacttcg ggccagcgtg gcacaaagac caatggtgaa ctgccatgac
    36121 aggtattggc cccagcagcg gaactcacaa ggcaccataa cgtcccctcc ctgataacgc
    36181 tgatactgtg gtcgcggtta tgccagttgg catcttcacg taaatagagc aaatagtccc
    36241 gcgcctagct ggcggtttgc caaagccgtt gcgactgctg ccagtattgc cagccataga
    36301 gtccacttgc gcttagcatg accaaaatca gcatcgcgac cagcgtttca atcagcgtat
    36361 aaccacgttg tgttttcatg ccggcagtat ggagcgagga gaaaaaaaga cgagggccag
    36421 tttctatttc ttcggcgcat cttccggact atttacgccg ttgcaggacg ttgcaaaatt
    36481 tcgggaaggc gtctcgaaga atttaacgga gggtaaaaaa accgacgcac actggcgtcg
    36541 gctctggcag gatgtttcgt aattagatag ccaccggcgc tttaacgccc ggatgtggat
    36601 cgtagccttc aatctcaaag tcttcgaaac ggtagtcgaa gatggattcg ggtttacgtt
    36661 tgataatcaa cttcggcagc ggacgcggtt cgcggcttaa ttgcagatga gtttgatcca
    36721 tatggttgct gtacaggtgc gtatcgccac cggtccagac aaaatcaccc acttccagat
    36781 cgcactgctg cgccatcata tgcaccagta acgcgtagct ggcaatgttg aacggcaggc
    36841 cgaggaagac gtcacaggag cgctgataaa gctggcaaga gagtttgccg tctgccacat
    36901 agaactggaa gaatgcatgg cacggtgcca gcgccatttt atccagttcg cctacgttcc
    36961 acgctgaaac aataatgcgg cgcgaatccg ggtcgttttt cagctggttc agtaccgtag
    37021 taatctggtc aatatgacga ccatctggcg ttggccaggc gcgccactgt ttaccataca
    37081 ctggcccgag gtcgccattt tcatcggccc attcgtccca gatggtgaca ttgttttcgt
    37141 gtagataagc aatgttagta tcgccctgca ggaaccacag cagttcgtgg atgatggaac
    37201 gcaggtggca acgtttggtt gtcaccagcg ggaatccatc ctgcaggtta aaacgcatct
    37261 gatgaccaaa aatggaaagc gttccggttc cggtacggtc gtttttctgt gtgccttcgt
    37321 cgagcacttt ttgcatcagt tctaaatact gtttcatggt tcctcaggaa acgtgttgct
    37381 gtgggctgcg acgatatgcc cagaccatca tgatcacacc cgcgacaatc atcgggatgg
    37441 aaagaatttg ccccatgctg atgtactgca cccaggcacc ggtaaactgc gcgtcgggct
    37501 ggcggaaaaa ctcaacaatg atgcgaaacg cgccgtagcc aatcaggaac aagcctgaga
    37561 cagctcccat tgggcgcggt ttacgaatat acaggttgag gataataaac agcaccacac
    37621 cttccagcag cagttcgtaa agctgtgatg ggtggcgcgg cagcacaccg taagtgtcga
    37681 aaatggattg ccactgcggg ttggtttgca gcagcaaaat atcttctgta cgggagcctg
    37741 ggaacagcat ggcaaacggg aagttcgggt caacgcggcc ccacaattca ccgttaataa
    37801 agttgcccag acgcccggca ccaagaccaa acggaatgag tggtgcgata aaatcagaga
    37861 cctggaagaa ggaacgttta gtacggcggg cgaagataat catcaccacg ataacgccaa
    37921 tcaggccgcc gtggaaagac atgccgccgt cccagacacg gaacagatac agcggatcgg
    37981 ccataaactg cgggaaattg tagaacagaa cataaccaat acgtcccccg aggaagacgc
    38041 cgaggaagcc cgcatagagt aagttttcaa cttcattttt ggtccagcca ctgcccggac
    38101 gattcgcccg acgtgttgcc agccacattg caaaaatgaa acccaccaga tacatcaggc
    38161 cgtaccagtg aagcgccacg ggtcctattg agaaaatgac cggatcaaac tccggaaaat
    38221 gcagatagct actggtcatc tgtcaccaca agttcttgtt atttcgctga aagagaacag
    38281 cgattgaaat gcgcgccgca ggtttcaggc gctccaaagg tgcgaataat agcacaaggg
    38341 gacctggctg gttgccggat accgttaaaa gatatgtata tgatccgcgc tataaccctc
    38401 cgcgaatcag cccgcccatg ccgcgacgct ccataaaggc tgcaacctga tgacgaactt
    38461 cggtcgccag ttgcgcttcc agactacgct gcgcaagatt ttctgcttcg gcaaaatcaa
    38521 tgcgccgcag caggtatttt acccgcgcta cagaacgtcc gttcatagac agatggcgat
    38581 acccaagccc aatgaggatt gccacgcaca tgggatcgcc cgccatttca ccgcacaaac
    38641 gaagatcgat accatgtatt tccgcttccc gggcgatcat cgccagagct cgtaacattg
    38701 caggatgaag actgtcgtaa atgttcgcca cccgggtatt gttgcgatca acggccagaa
    38761 tgtattgagt cagatcgttg gtgccaacag agatgaaatc gacccgcttt gccagatgcg
    38821 gcagcataaa taccattgac ggcacttcca gcatgatgcc gatacgtggt ttgggaattt
    38881 cgtaaccgat catctcctcg acttcacgtc cggcacgttc aatcaggcgg cgtgcttcgt
    38941 caacttcatc gaggctggtg accatcggca acagaatatt caggttgccc gtagcggcat
    39001 tagcacgcag catcgcccgc acctggatca agaagatctc cggctgatcg agcgtaatgc
    39061 gaatcccacg ccaacccagg catggattct cttcgctgat cggcatgtaa ggcagctgct
    39121 tatctgctcc gacatccagc gtacgcaagg tgacgggttt atcattgaac atttgcagca
    39181 tcccctgata ctgcgccacc tgttcttctt ccgacgggaa accactttgc agcatgaatg
    39241 ggatttcagt gcgataaaga ccgatgccat caatacagct gcccagtttt tcttcatgtt
    39301 ccgggcttaa accagcattg agcatgactt ttatacgctc accgcttttt aactgcgcgg
    39361 gtaaattgac gtcatcttcc gccagacggc taagctcaat ctcttcacta attagccgct
    39421 gatattcttg cagcagtacc ggctccggat cgaccagcaa ttcaccgcga tagccatcaa
    39481 cgatcagcgt cctacgatgc agcaccgaag gctgaatatc cgcgcccatc acggtaggga
    39541 tccccagcgc acgtaccatg atcgcagcat gggagttggc tgagccatct cgcacgacaa
    39601 caccgactaa gcgatcctgg ggcagctcag caagagttgt cgctgacagt tcatctgcca
    39661 ccagaatgaa acgttccggc caggcgttcg gcccttgatt agcgtcatca agatgaaaca
    39721 gcaatcgctg acccagcgca cgtaaatcgc cagcccgctc tttgagatag ttatcgctta
    39781 gcgcggcaaa ctgttcggca aatttttcaa tgaccgtttt taccgcccac tctgccaccg
    39841 agcctttatc aacctcggca aacaattcgc gacgcagccg ggtgtccgaa agcaggtgcg
    39901 agtaaagatc gaaaatagcc gccgtttctt tttgtgctcc ggcggcaaag cgtttgctgt
    39961 agcggcgaaa ctcgtttgcc gcttcttcca gcgccccggt cagtcgttcg cgttccagag
    40021 ccggatccag cgttgatgcc tgatacacct gttccattaa aggtaacgtg gcatcctgcc
    40081 agccttcggc aatcgccaca ccaggtgctg ccggtaatgc gcggattcgc gtctggcgat
    40141 attgcccaaa cagggcagtc aactgcgact gagaaagaat agctgccatc tgggtggcaa
    40201 gcgtcaccag gaaggattct tcactttcgt catactggcg caactctcgt tgctgtacca
    40261 ccagtacacc aagcaactgg cgacgttgaa taattggtac gcctaaaaac gcgcggaaac
    40321 gttcttcttt tacggagggg atgtatttga agctggggtg cttttgcgca tctgcaaggt
    40381 ttatcggttc cgccagcctg ccaaccaggc cgacgatccc ttcatcaaac gcgagcgtta
    40441 cagtgcgacc gcgtggtttt ttcagccccc gggtcgccat caggtagtaa caacgtcgat
    40501 catgatcggc caggtagacc gaacagacct cggtatccat cgcaagacag atgtcggtaa
    40561 ccagaatatt taacgcctca ttcaggcgtg gtgcgctggc taccttttcg actatttcgc
    40621 gcaggcgagt gagcataatt ggcgtgactt aacctctttt acgtcgataa gcagatgcgt
    40681 tttgtggttt tggcgtattt tcctgcagtg acatcaccac actcgcgaac tctttcatta
    40741 ccctacggta gacatcacgt ttaaatgaca ccacctgtct gaccggatac cagtaactta
    40801 cccatcgcca gccgtcaaac tctggtgtac tgctggtttg catattgatt tctgcatcgc
    40861 cgcttaccag ctgcaagaga aaccattttt gtttttggcc gatacaaacc ggcttcgtgt
    40921 cccaacgcac caaacgtttc ggtaatttgt agcgcaacca gttacgcgtt gaagcaagga
    40981 ttcgaacgtc tttgcggctt aatcctactt cttcaaacaa ttcacggtac atcgcctgct
    41041 ctgcggattc tccggggttg attccgcctt gcggaaattg ccaggagtgc tgaccaaatc
    41101 gccgggccca cattacctgc ccctggcgat tacaaatcac gatacctacg tttgggcggt
    41161 agccatcgtc atcaatcacc ggactacctc aaaataaagc tttatatacg aatgattgtt
    41221 tcatactcca ggaagacggt aaaccactct ctgcagggca ttacacacta ataacaattg
    41281 aataactcac agttatgtgc agagttataa acagaggaag gggtggatag ccgtttttat
    41341 tcaccttttc tgtggataga gttgtgaaga actacggaat tactacggga aaacccggag
    41401 cattctgaat aagcccgata tagaaatttt aaaatattca ttattagtca atgcattaca
    41461 tgtaatttga gtaatgaaaa agagtaattc gtgacccagg tcacacctct catttacggg
    41521 ttggcgaaag atcaaccaat gccgtattta tccacagaat gtgccactaa gttaagcact
    41581 gaaccactaa aaactggagt ttcgtcgcac gtcaaggctg taaatgaaaa cagtagtgga
    41641 ggtttttcac agttatccca gctttctgtg gataacatgg tgtaagatcc tgtttatttt
    41701 cagtgaccag atttggaaaa cctattgcag tgttgcgcaa ctcgattacc ggcagcctaa
    41761 aaagcaatat aaatcagaga attgaacaac acatgtggaa aaagttacac tgcgaatatt
    41821 cggcacataa ttactgtttg ttttttaacc aaggtatcat gacatgtccc aacctcgccc
    41881 actgctctct cctcccgaaa ctgaagaaca gttgttagcg caagcacagc aactttctgg
    41941 ttatacattg ggagaactgg cggcacttgc cgggctggtt acgccagaga atttaaaacg
    42001 cgataagggc tggattggcg tgttactgga gatctggcta ggtgccagcg cagggagtaa
    42061 acctgagcaa gattttgctg ctctgggcgt ggaacttaaa actatccctg tggatagtct
    42121 tggtcgtccg ctggaaacaa cattcgtttg tgttgccccg ttaacgggta atagcggggt
    42181 gacctgggaa accagccatg tgcgccacaa gctcaaacgc gtactgtgga taccggttga
    42241 aggcgagcgc agcatcccgc tggcgcagcg tcgcgtagga tcaccgttgc tgtggagccc
    42301 gaatgaagag gaagaccgac agctacgcga agactgggaa gaattaatgg atatgattgt
    42361 tctcggtcag gttgagcgga tcaccgctcg tcacggggaa tatttacaga taagaccgaa
    42421 agcagcgaat gcgaaagcgc ttaccgaagc cattggtgcc cggggcgaac ggattctgac
    42481 gctgccacgc ggcttttatt tgaagaagaa tttcaccagt gcactactgg cccgtcattt
    42541 tctgatccag tagccatcgc tttgacctgc cgctttccgg gcatataatt accgcttcat
    42601 ttttttggca gggcttttta gatgttattt gcatggataa ccgatcctaa cgcttggctt
    42661 gcgctcggta cgctgacgct gctggagatc gttcttggga tcgacaatat tattttcctt
    42721 tctctggtgg tggcaaagct tcccacagca caacgtgctc atgcgcgccg tctggggttg
    42781 gcgggagcca tggttatgcg tctggcgctg ctggcatcaa tctcttgggt tacgcgcctg
    42841 acaaatccgc tttttacaat attcagtcag gaaatttctg cccgtgattt gattctgctt
    42901 ctgggtggct tgttccttat ctggaaagcc agcaaggaaa tccacgaatc cattgaaggt
    42961 gaagaagaag ggctgaaaac acgcgtttca tcattcctcg gcgctatcgt gcagattatg
    43021 ctgctggata ttatcttcag cctcgactcg gtgattaccg ctgtgggtct gtcagatcac
    43081 ctgtttatta tgatggcagc cgtggtgatt gccgtaggcg tgatgatgtt cgccgcgcgc
    43141 tcgattggtg attttgtcga acgccatcct tcggtaaaaa tgctggcgct ctctttcctg
    43201 attctggtgg gctttaccct gattctggaa agtttcgaca tccacgtacc gaaaggttac
    43261 atctacttcg cgatgttctt ctctattgcg gttgaaagcc tcaacttgat tcgcaacaaa
    43321 aagaatccgc tctgatactc cgtacgctct cctgcgggag ggcgtactca ctccttttac
    43381 cctcacagtt taagattttc ctgctttcaa gatatataac gtcggtttat aaacagacta
    43441 ttatcatagg taggccaaat gaggataacc ggatgaaaaa atgggcagta ataatttctg
    43501 cagtcggact ggcgtttgct gtttccgggt gttccagtga ttacgtcatg gcgaccaaag
    43561 atggccgtat gattttgacc gatggaaaac ctgaaattga tgatgatacc gggctggtga
    43621 gttatcacga tcagcaaggt aacgcgatgc aaattaaccg tgatgatgtt tcgcaaatta
    43681 ttgaacgtta acaaataagg tcagcatccg gctggcctta agatttttct ctttcccttt
    43741 tcccttccct ctgccatttt tatattcctt atgtcgtgat tataaaaagg aaacggctat
    43801 gcaatatcac cgtatacccc acagttcgct ggaagtcagc acgctggggc ttggcacgat
    43861 gacgtttggt gaacagaaca gcgaagccga cgcccacgca caactcgact atgccgtcgc
    43921 tcagggcatt aaccttatcg acgttgccga aatgtaccca gtacctccgc gccccgaaac
    43981 gcaagggtta accgaaacct acgtcggcaa ctggctggcg aaacatggca gccgcgaaaa
    44041 gttaattatc gcctccaaag tgagcggacc gtcgcgcaat aatgacaagg gcatccgccc
    44101 ggatcaggcg ctggatcgga agaatatccg cgaagcgctg catgacagcc tcaagcgcct
    44161 acagactgat tacctcgatc tttatcaggt gcactggccg cagcgcccga ccaactgctt
    44221 cggcaaactc ggttatagct ggacggattc tgcgcctgcg gtttcgctgc tggatacgct
    44281 ggacgcactg gcagagtacc aacgcgcggg aaaaattcgt tatatcggcg tgtcgaacga
    44341 aactgcattt ggcgtaatgc gctacctgca tctggcggac aaacacgatc tgccgcgtat
    44401 tgtcaccatt cagaaccctt acagtctgtt aaaccgcagt tttgaagtag gtctggcaga
    44461 agtcagccag tatgaagggg tcgaactgct ggcctattcg tgcctgggtt tcggcacgct
    44521 gaccgggaaa tatctcaatg gtgcaaaacc cgctggcgca cgtaatacgc tctttagtcg
    44581 gttcacccgc tatagcggtg agcaaacgca aaaagccgtc gcggcgtatg ttgatatcgc
    44641 cagacgtcat ggccttgatc ctgctcagat ggcgctcgcg tttgtacgct gtcaaccgtt
    44701 tgttgccagc actctgctgg gcgcaaccac gatggatcag ctgaaaacta acatcgaaag
    44761 tttgcatctg gagttaagcg aagacgtatt agctgaaatt gaagcggtac atcaggttta
    44821 tacttatcct gcaccataaa gattaacgcc ggttaaaacc ggcgttaaat attaatgacg
    44881 gcgctgccag atccacagcg ccgttattgc cagcgcaaat agcgcaccga agccaatgcc
    44941 aatgggcacg accgggatgc ctaccattac cgccagcgag taaatgccca gcatcaacaa
    45001 catggcgctg ttttcgccaa ggttttgtac tgcaatcgca ttccccgccc cgacgctttt
    45061 tttaccccgc tcctgtagca acgcattgag cggaacgaca aaaaagcccc ccagcacgcc
    45121 aatcagcatc aacaaggcat aggctggcag cagctcgtgt tgcagggaga aaatcggtac
    45181 caccacgcca atcaaaatcc cggctggcat acagcgtgac acggtttcca gcgtaaccag
    45241 cttcgctgcc gcacctgcgc caaccacgat accaatcact accatcgcgt tgagataggt
    45301 cggcgtagcg ttatcggtaa tgcccagcgc caccggtacc cacagcacca acaggaaacg
    45361 cagcgtgaca cccgctcccc agaataaact ggtgcccacc agcgaaaaac gcgtttcacc
    45421 attgcaccat agcgaggtgc aggcattcag gaaactgcgg gtcatgttga tgagattcca
    45481 ggactgcccc ggacgcgccg ccagtttggg aatgtagata ttggcaacga ccgcaccacc
    45541 gtaggccagt gcgcatgcgg ccagagcgac caggacatgc cagtcagcca gcacaccacc
    45601 ggctacggaa ccgagtaaaa tcgccgctat ggcagaagct tccattaaac cgttagcttt
    45661 tactaactta ctacccgtgg ttaattcgcc gagaataccg tatttcgccg gtgaataggc
    45721 tgcagcacca acacccacca gcgtatagcc gagaaacgga ttgataccaa agcagatact
    45781 ggctgcgccc agcagcttca ggccgttggc aaacatcatc acccggcctt tggcgaagct
    45841 atccgccacc tgcccgacaa acggcgccaa aagaatgtaa gcacctacaa acaccatttg
    45901 caggatgggc tggctccact ccggatagaa ctgcgctttc agtaacgtca gagtggcaaa
    45961 cagtagcgcg ttatcgccaa acgcagagag aaactgcgcc acgataaccg ctttcatccc
    46021 cttcgaccac aacgaagtgt taatgtgcac tgactcactc atcgtgttgt tccgcttcgt
    46081 ctacccagct tttcaacgtg acaaagtcag gtttgccgct gccaagtaat ggcatctgtt
    46141 tcagatagcg aatatcgcgc ggtacagcaa gctccggcac gccgtgctcg cgggcatact
    46201 gttgcaactt atcgcgcgtc agttcgttat ctgtggtgaa aagcaccagt gcctcgcctt
    46261 tgctggcatc gctcttaatc gcagtggcat ggactttatc tggcgaaaca ccaagtgcca
    46321 gttgttccac catttccagc gacaccattt cgcctgcaat tttggcaaag cgttttgcgc
    46381 ggccctgaat ctgcacaaag ccctgctcgt caaaacgcac aatatcgcca gtgtcatacc
    46441 agccgcgctc catttcgccg cgaacattct cggcggtggg cacttccagt acacctggct
    46501 tctccacccg cagatagccg ttcattatgt tcggcccttt cagttgcagg cgtccgccct
    46561 cttcgatacc agggaccgac aacaggcgtg catccattcc tggtagaata cgccctaccg
    46621 taccgggttt cgccgccatc ggtacgttga tagaaacgac aggcgcgcat tcggtcacac
    46681 cgtagccttc aaggatgcgc aggccaaatt tatcctgcca aagctgtttg gtactttctt
    46741 gtagtttttc tgcgcctgcc accacatagc gtagacgata gaagtcatac gggttggcga
    46801 aacgcgcgta gtgaccgagg aaagtcgagg taccgaacaa cacggtgcaa ctgcggtcat
    46861 acaccaagtc cggcacaatg cggtaatgca gcgggcttgg ataaaggaac acttctgcac
    46921 ctgtaagcag tggcgtaaac aggcctaccg tcagcccaaa ggagtgaaac agcggtaatg
    46981 ccgacataaa gcgatcgttg gtggtgaagt cggcaatcgt tttaatctgc tcgacgttcg
    47041 ccagaatgct tttatggcta tggacgacgc cttttggatg gccttcagaa ccggaggtaa
    47101 agaggatcag cgcctcttct tccggctgct gttttacctg cgccaggcgc ggcatcagca
    47161 aatgagcgaa gatccatact ttgtcggcag tggtgacatc tgcttttaaa tcttccagat
    47221 agacccagcg cacctgagta agttgctccg gcagatgcca gagtttgcct ttatcgagaa
    47281 actggcggga agtgaagatg gttttgattt cagccgccgt aatagcactg gtcagccctt
    47341 ttaccccggc agtgtagttc atcattgcgg gaatgcggcg acgcgcgatg gccccaaaaa
    47401 tcactgccgc actgatgcct gcattgggca gcattaagcc gatgcgttcg ccttcaacac
    47461 tgtatttttc aaggatgcgt ccaacaaaca gcgttttcgt aagcaatttg cgataggagt
    47521 ctggggtaaa gttgacgtct tcgacacatt tcttcccggc tccgaagcgg tacattgcac
    47581 tcagtaaaga ttcgtacaga gtttcacgcg ggcgcaccgc catacgcgct tccatcatta
    47641 tttgatgcag catttcgcca gcgattttgc gacggtcacg ggcacgcggc gcatccggca
    47701 tcgccacctg cgttggaggc aaaatatgca gagtaatttg cgggaacaag cgacgtttaa
    47761 ccagaccttt caggcggctg aagtgcgtaa gttccgcccc ttcaatacgc acaggaataa
    47821 ccgttgcacc agacttcgcc gcgacaaaac ccgcgccatc gtagattttc atcagcgagc
    47881 ctgtagtggt gatgcgtcct tcagggaaaa tcaccactgg tcggccctgt tccaccagac
    47941 gtaccagatg tttaatagcc ataggttgcg tcgggtcgag aggaacaaag tcgataaatg
    48001 atttcagcca acgcatatac cactgttggc ttattgaggt gtaaacggca aacactggac
    48061 gcacaggtaa aaacagtcca agcaaaatgc catcaataaa agagacgtga ttaggcgtaa
    48121 ttagtacgcg ctcgcccttc agtgcctggg tgtcacccgt aacgcgaacg cgatacaaaa
    48181 cacggcacaa atttcgaaaa aagctaaaaa gcattccaac tccctttgct ctgattcagt
    48241 aaaagcgaat ggagggagat tacacgagat aaagaacgcg agcgacagta aattaggtgc
    48301 gaaaaaaaac ctgcgcatcc gcgcaggttg gtgcaagaga cagggtacga agagcgtacc
    48361 gaataatctc accaatcaat acctctggga tcttgattgt ggtctgcacg acgactcttc
    48421 gccagcgaga aaacgcaaag gaatgaaggg aaatgcaacg aggtgtgtaa attgtcggtt
    48481 actgttacag attgatgacc ggcaaaaaaa aacctgcgca tctgcgcagg ctggtgtaat
    48541 tcatgtgctc aacacgaagt tgacttcacc tatcaatacc tctgggatca ccactttagc
    48601 aacctgaagc caaacgccac cagcggtcaa tcgcaacagc gtttcgcaaa gtgtaaccaa
    48661 aggtttgcaa tctctttttt tgtcttactt atttgcgttt ggctcacatt cccacgatga
    48721 aaacacgcca ccccttgaac caacgggcgt tttccgtaac actgaaacaa tgtaagcgtt
    48781 tacccactaa ggtattttca tggcgaccat aaaggatgta gcccgactgg caggcgtttc
    48841 agtcgccacc gtttcccgcg tcattaataa ttcacccaaa gccagcgaag cttcccggct
    48901 ggctgtgcat agtgcaatgg agtctcttag ctatcacccg aacgccaacg cccgtgcgct
    48961 ggcgcagcag accactgaaa cggtcggtct gatcgttggt gatgtttccg atccgttttt
    49021 cggtgcaatg gtgaaagcgg tcgaacaggt ggcttatcac accggtaatt tcttattgat
    49081 tggcaacggt taccacaacg aacaaaaaga gcgtcaggct attgagcaac tgatccgcca
    49141 tcgctgtgct gcgttggtcg tccatgccaa aatgatcccg gatgccgatt tagcctcatt
    49201 aatgaaacaa atgcccggta tggtgctgat caaccgtatc ctgcctggct ttgaaaaccg
    49261 ttgtattgct cttgacgatc gttacggtgc ctggctggca acgcgtcatt taattcagca
    49321 aggtcatacc cgcattggtt atctgtgctc taaccactct atttctgacg ccgaagatcg
    49381 tctgcaaggg tattacgatg cccttgctga aagtggtatt ccggccaatg atcggctggt
    49441 gacatttggc gaaccagacg aaagcggcgg cgaacaggca atgaccgagc ttttgggacg
    49501 aggaagaaat ttcactgcgg tagcctgtta taacgattca atggcggcgg gcgcgatggg
    49561 cgttctcaat gataatggta ttgatgtacc gggtgagatt tcgttaattg gctttgatga
    49621 tgtgctggtg tcacgctatg tgcgtccgcg cctgaccacc gtgcgttacc caatcgtaac
    49681 gatggcgacc caggctgccg aactggcttt ggcactggcg gataatcgcc ctctcccgga
    49741 aattactaat gtatttagtc cgacgctggt acgtcgtcat tcagtgtcaa ctccgtcgct
    49801 ggaggcaagt catcatgcaa ccagcgacta accgcagtta aagcaattcc agcgccagta
    49861 attcttcgat ggtctggcga cggcgaatca accgcgcctg accattatca aacagaactt
    49921 ctggtaacag cggacggcta ttgtagttgg atgacattga tgcgccatat gcccctgtat
    49981 catgcagtac cagataatca cctgccttca cttccggcaa ggcgcgggtt tcaacatttc
    50041 ccccttcctg ctgggtaaag acatcgcccg attcacataa cggcccggcg acgacggttt
    50101 ccaccgttgg cgcgtgttcc agagaacgac catcagctgc cagggcactg atatggtggt
    50161 aactaccgta cattgccggg cgcatcagat cgttgaaccc ggcatcaacc agcacaaagt
    50221 ggcggctacc catttgtttg acgctccgca cctgagtaat taatacgcca gactgcgcta
    50281 ccaggaagcg acccggttca atttccagtt tcacagggtg gcccaaatgg cgagcgattt
    50341 gctcacgcgc ggcattccac agaccataat aatgttcggt atcaaccgcc tcttcaccct
    50401 gttgataagg aatagaaagc ccaccgcccg cagaaatagc ctgtaaatcc tgaccgaatt
    50461 cgataacctg acgcaccata gcaccacaca cctgctccag atgggcataa tcaacgccag
    50521 aaccaatgtg catgtgaatg ccgactagct gcagatgatg acgttgtatc acgtccagtg
    50581 cggcgggcag atcggtgtac cagataccgt gcttgctgtt ttcgccaccg gtattggttt
    50641 tttggctatg tccgtgacca aaccccggat taacgcgcag ccatacccga tgccctggcg
    50701 aaacctggcc cagttggtcg agcgtatcaa cagaacccgc attcaccgga atttgcaatt
    50761 cactgacgcg ttcaagcgtc gcctgatcga taacatctgc cgtaaaaaca atatcatcgg
    50821 ggtgcgtttg cggattgtaa cccgccgcca acgcacgctc tatttcgcct aacgagacgg
    50881 aatccacttt cacgccctgc tcacgcatta agcgcaaaat atgaatattg gaacaggctt
    50941 tctgtgcaaa gcgcaccact tcaaactgtt tcagcgctgc aatctgccga cgaataattt
    51001 gcgcatcgta gacccacacc gggcagccaa attcagcggg caaacgcagc agattttcgg
    51061 cggtgagatc ggtatcggtg ctgaacagtg aatgtggcat aacaaactcc agatgagtgc
    51121 ttttttatga ttacgccaca tcataaaaag aataaaaaat atcgatttat gtcgagtcta
    51181 tgcaaaaatg atatggatta ccggattgcg agagagcgct aatggccgcc gttaacttac
    51241 gtcatattga aatttttcat gcggtaatga ccgccggaag cctgactgag gcagcacacc
    51301 tgctacacac ctcacagcca accgtcagcc gcgaactggc gcgctttgag aaggtgatcg
    51361 ggctgaaatt gtttgagcgc gtacgtggac gattacatcc taccgtgcaa ggactgcgtc
    51421 tgtttgaaga agtgcaacga tcctggtacg gactggatcg cattgtcagc gccgcagaaa
    51481 gtctgcgcga gtttcgccag ggagaactgt ctattgcctg cctgccggtc ttttcgcaat
    51541 cttttttacc gcagctcctg caaccctttc tggcacgtta tcccgatgtc agcttaaata
    51601 tcgtgcccca ggaatcaccg ctacttgaag agtggctctc ggcccagcgt catgatttag
    51661 gactcactga gacactccat acgcctacgg gaacagaacg taccgaatta ctctctttag
    51721 atgaagtgtg tgtgttacct ccgggtcatc cgctggcggt aaaaaaggta ttaacgccgg
    51781 atgattttca gggtgagaat tacatcagcc tttcccgtac tgacagctat cgccagttgc
    51841 tggatcagct atttactgaa catcaggtta aacgacgcat gatcgtagaa acccacagcg
    51901 ccgcgtcagt ctgcgcaatg gtacgggcgg gggtaggtat ttcggtggtt aacccgctca
    51961 ccgcactaga ttatgcggca agcggtttag tggtgcggcg gttcagtatt gcggttccgt
    52021 tcaccgtcag cctgatccgc cccctgcacc gcccgtcatc agcgctggtt caggcgttta
    52081 gtgggcattt acaagcgggg ttaccgaaac tggtcacttc tcttgacgct attttgtcgt
    52141 cagctacgac agcataaaag cgacagcatc ctcggcatgg atcgccgcgg tatcaaacac
    52201 aggcaaaaca ctgcgctctt ctggcaccag taaaccaatt tctgtgcagc caaaaatgac
    52261 gccctgtgcg ccctgttctg caaggcgagc aatcacttgc gcataataag cgcgtgacgc
    52321 ttcggtaaat tgccccagac acagttcttc aaaaataatc tgattaattt tcgcccgttc
    52381 atccgcttca ggaataagac aattgatgga aaattgttcc gtcagccgcc cgcgataaaa
    52441 atcctgttcc atggtgtaac gcgtacccag cagcgccaca cgagtcattc cggccccggt
    52501 aattgcacgt ccggtggcat ccgcaatgtg taagaaaggc agagtgcaac gtgactcaat
    52561 ggcatccgcc actttatgca tcgtattggt acataacaca ataccttctg cgcccgcccg
    52621 ctgtaagcca agcgccgcct cagccagaat gtccccggtt ttatcccatt ccccgcgacg
    52681 ctggcactct tctatttcat gaaaatcgac gctatgtagc agcatttgcg cagagtgaag
    52741 cccaccaagc cgctgtttaa tgccttcatt tatcagacga tagtaaggaa tggtggattc
    52801 ccagctcatt cctcccagca aaccaattgt tttcattcct ctctcctgat gtgtgttacc
    52861 gcaacaaggc tgaataagga acgagacaaa cgcctcaacg gccaggtgcc caatctctat
    52921 taacgaaaaa agggccggat gtacagcaca tccggcccgt gaaatcagac gccgatattt
    52981 ctcaacttct cgcctgccat cagtttgcgt tcgatatgtt ccagcgtgac atttttggtt
    53041 tccggaatga gccagaaagt aatacccaca aacgcaatgt tcagcgcagt gtagagccag
    53101 aacgtaccgg cagcgccaat gctatcaagc agtgtcagga aggccgcgcc gataatcata
    53161 ttcgacaccc agtttgtcgt cgtcgaacag gtaataccga aatcgcggca tttcagcggc
    53221 tgaatttcag agcacaggat ccacaccact ggcgcggcgc tcatcgcata accggcaata
    53281 cacatcatcg tcatgccaac agagagccag gacaagccac tggaagccgt accgttatca
    53341 aactgcatca ggcaatagcc cagcaccaga gtgcctaacg ccatcacgct gaaaccaatt
    53401 ttcagagccg gtttacgacc tgctttatct accgtaaaga ccgcaatgaa ggtcgcgaac
    53461 ataaaggtca gtcccacgac cagagtcgca atcatctgtt gttctgtggt cgtaaagccc
    53521 gccattttga agatacgcgg cgcgtagtac atgatgatgt tcataccggt aaactgctgc
    53581 atcgcctgca acaacatacc gagaaacaca gcacgacgga cgttacggtt gatcttaaac
    53641 agtgcccaac cgccctgttt taacttcagg ctttcacgaa tttcgttgag ttcttctcgc
    53701 gctttttccg acgtatcgcg cagcatacgc aacacttctt ccgcctcaat atgacgcccc
    53761 ttttccgcca gccagcgcgg gctatttggc aggaagacca ccagaataat cagcagaact
    53821 gctggtaaag caagaccccc caacattgcg cgccagttac cgctataact gaacgctgta
    53881 tcggataaaa acgccagcac gatgccgagt gtgaccatca actggtacat actgatcatc
    53941 ttaccgcgaa cgttttcact tgccatttca gaaagataca gaggagcggt gtaagacgcg
    54001 atcccgacag caatgcccag caccacacga gcggcgatta acatctctac gctggtcgca
    54061 aaagcggacc ctatagaacc gagtacaaac aggatggccc ccgccatcag gctgtattta
    54121 cgccccaggc ggaacgacag ccaaccatta aacagcgcac caattgctgc gccgagcatc
    54181 atgctactaa ccacccattc ctgcaaacga ctggtcagca caaagtgatc ggtaatgaac
    54241 ggcaacgctc cggcgattac gccgatatca agaccaaata acaatcctgc gaccgcagca
    54301 gctaccgaaa caaacatatt catacgccgc gtatcacgca aagaacgtgg cgttaaagca
    54361 gattccgtat tgatagtaac cattttttcc tgccagcaga gagtaagaca ttgtgaaaaa
    54421 atacgtgaac aattcacaca ggtgtcaggt cggaaacagc ataaatatgg attaaattgc
    54481 tgcgacatgc cgttatgtga tggatattcc aattttaaaa ttaagttgaa ttattgagat
    54541 tattattaac ctcctaattt taaagcagat aaaattcata aagttcatta attgataatt
    54601 gatatggatt atttcataac catgatatgg attatgatga tctagagata taaaaaaccc
    54661 tgccatgcgg cagggtcata aaagtaagag gaatgaatta acgcgccagc caaccgccat
    54721 ccacagcaat ggcataacca tttacataat ctgaagcgct ggaggcaaga aacactaccg
    54781 gccccatcag gtcactcggc agtccccaac ggccagctgg aatgcgatcg agaatttccg
    54841 cgctacgttg ttcatctgcc cgcagttgtt gggtattgtt ggtcgccatg taacccggag
    54901 ctatcgcatt aacattaatg ttgtgtttag cccattcgtt tgccatcaat cgcgtcacac
    54961 ccatcacgcc gctttttgat gcggtataag aaggcacacg gatcccgccc tggaaggaga
    55021 gcattgacgc gatattgata atcttgccgc cattgccttg cgcgataaag tgtttcgccg
    55081 ctgcctgaga catgaagaat acgctcttga tattcaggtt catgacatcg tcccagtctt
    55141 tttcgctgaa ctcgagagcg tcttcgcggc gaatcaatcc ggcgttattc accaggatat
    55201 caatatgacc aaattcagct accgcgcgat ccagcagtcc aggaatgcca tcaatctttc
    55261 gcagatcggc ggtcaggctt aaaaaacgac gcccctgcgc cgtgacctgc tcgatggttt
    55321 cagtcggttc aacgatgtta atgccaacaa tgtcacagcc cgcttgcgcc agccccaacg
    55381 ccatcccctg gcccagccca gtatcacaac cagtgacgac cgcaacttta ccttggagag
    55441 aaaatgcact taaaatcata acaacacctt attcgtttat gcccacaact agcgcaaatc
    55501 tttaacgccc acatggtcca tatcattaaa gacctggttt tcaccgacca tgccccagat
    55561 aaaggtataa gctttggttc cgacgccgga atggatcgac cagctcgggg agatcaccgc
    55621 ctgctcgtta tgcatcacaa tgtgacgcgt ttcttgcggc tgccccatca tgtggaaaac
    55681 gcaggcgtca tcatccatat tgaaatagaa ataaacttcc atccggcgct cgtgggtgtg
    55741 acacggcatg gtgttccaca agttacctgg agccagctcc gtcagcccca tactcaattg
    55801 acaggtttcc agtacatccg ggacaaaata tttgttaatc gtgcgacggt tactggtgag
    55861 gttatcgcct aacgtgactg gagatacttc gtccggtgtg acttttttgg tgggatacgt
    55921 cgtatgcgcg ggtgcgcaat tgtaataaaa cttcgccgga gtggcggtat caatactggc
    55981 aaagacaact tcttttgcac ctttaccaac atacagggcg tcgcggtgac cgatttcata
    56041 gcattgacca tcgacagtaa tcgtaccggc accgccaata ttgataacac ctaactcgcg
    56101 acgttcaagg aaatagctta cgccgagttg tttaccaact tccccgccaa cggaaaccgt
    56161 tttagttatc ggcataatgc cgccaacaat aattcggtca atgtggctgt aaaccatggt
    56221 gtactcatcg gcgacaaata ccttttcaac caaaaattca ttgcgcagcc cttgggtatc
    56281 cagcgttttt gcgtgcgcac cgtggatgct ctgtcttacg tccacatcaa cctccgataa
    56341 tcagtgaagc gtgaaaatag aaaacgatag tgaaacactg tttcgttttc tctataaagg
    56401 gatgatatgc ccatgggcta gggttttcaa ttaaaaataa aacaatgttt tatttttaat
    56461 ttaaggatta aaaaaagtgc agtgtcgatc acgaatatgt catgagaatg gaaaaatgag
    56521 ggagcccaga aaatgtatgt cgacttgcca gccatcatta ttcaacagat taatgacggc
    56581 agatatttat cggtaaggag gaaatgcaga gctactcgtc acgttcaatg gtcaatgcca
    56641 cgccctggcc cccgccgata caaagcgttg ccagcccttt gcgggcatta cgtttcacca
    56701 tttcatgaac cagagaaacc aggattcggc aaccggaagc gcctatcggg tgaccgagtg
    56761 cgatcgcgcc accattgaca ttgacccgac gctcatccca ctcaagcatc ttgccaaccg
    56821 aaagcgcctg tgcagcaaac gcttcattag cctcgataag atcgacatca gccaactgcc
    56881 agcctacacg ctccaggcaa cggcgggtcg catacaccgg cgcaattccc atcaatgccg
    56941 gatctacacc aacgctggca aatgcgcgga tgcgggccag cacgggtaaa ttcaacgctc
    57001 gtgctttggc ttcgctcatc atcattaccg cagctgcgcc atcgtttatg gatgatgcat
    57061 tccccgcggt cacagaaccg agactatcaa atgaaggatt taaacgggct aaaccttctg
    57121 cactggcgtc agtgcgtggc tgttcatcgg tatcaacaac caacgtctga ccgttacttt
    57181 ggatcattac cgggacgatc tcatctttaa atcgtccggc gtcaatcgcc gctcgcgctt
    57241 tttgttgcga gctaagtgcg taagcatcct gcaactgacg gctgatgcca tattcgcgag
    57301 ccagattttc ggcggtgaca ccaatatgat aatcattgaa ggcatcccac aacccatcat
    57361 gcaccagact gtcaaccaac tggctattgc caagctgtgc accggtgcgg ctatcagtca
    57421 gaacatgtgg tgcgcggctc atgttttcct ggccaccggc gatgacaata tcagcctcgc
    57481 cacactgtat cgcctgagta gccagatgca gtgctttaag accggaaccg caaacgtcat
    57541 taatcgtgat tgcagaaacg ctgttaggaa gaccaccttt aatagccgat tgccttgccg
    57601 gattctgccc tgcccctgca gtcaacacct gaccaagaat tacttcatcc accgcatatg
    57661 caggaacgcc ggtacgttct attaacgctt tcacgaccag actaccaagt tccacggcgg
    57721 aatgacccgc taacgcacca cgaaagcagc cgataggtgt ccgtaacgcc ccgacaatca
    57781 caacgtcttt catcacaacc tcgcaccaat taacagtgca atagtaaatg attgttaatt
    57841 acagttatct taattgttta aaaaaagtga tttttatcac aaaggaaata tgcctgagca
    57901 gtagtcagag tcataactgg cacgtaaggt ttgcaaccac taacccacca atagatgggt
    57961 agatagggcg ttaatctccc atacttaact tggtttatgg taaattgccc tccattttgt
    58021 ttaatttgta gatgatacgt tcagataatg tctaatattt ggtctaaaga agaaactctg
    58081 tgaagtttcg cgctctatgg cacagccgtt gatgcaggca cgctattcct tcctattcag
    58141 ttaggttcgg caggggctgt ggtcctgttt attactgctc tggtcgcctg gcctttaaca
    58201 tattggccac ataaagcctt atgccagttc atcctctcat caaaaacatc agcaggtgaa
    58261 gggataacgg gcgcggtaac acactactat ggcaagaaga ttggtaatct gattaccacg
    58321 ctgtacttca tcgccttttt tgtcgtcgtg ttgatatatg cagtggcaat taccaactca
    58381 cttacggaac agctggcaaa gcatatggtt attgatcttc gcatccgtat gttggtgagt
    58441 ctgggtgttg tattaattct gaatctcatt tttctgatgg gacgtcatgc cactattcgg
    58501 gtaatgggat ttttggtatt cccattgatt gcctatttct tatttctttc catttacctt
    58561 gtcggtagtt ggcaacctga tctattaaca acccaggtag agttcaatca gaataccctt
    58621 caccagatat ggatatcgat tcccgtgatg gttttcgcct ttagccatac gcccattatt
    58681 tctacgtttg ccatagacag acgtgaaaaa tatggcgaac acgctatgga taaatgcaaa
    58741 aaaattatga aagtcgctta tctcatcatc tgcataagtg tactgttctt tgtctttagc
    58801 tgcctgcttt ctattccatc ttcgtatatt gaagcggcta aagaagaagg ggtcaccatt
    58861 ttatcggcgc tttctatgct gccgaacgcc ccagcatggt tgtcaatttc cgggattatt
    58921 gtcgcagtag ttgcgatgtc gaaatcattc ctgggtacgt actttggcgt tattgaaggt
    58981 gccacagagg tcgtcaaaac aacactacaa caggttggtg taaagaaaag tcgtgcattt
    59041 aaccacgcac tatcaattat gttggtatcg ctgattacct tcattgtttg ttgcattaac
    59101 ccgaacgcga tttcgatgat ttacgcgatc agcggcccgc tcattgccat gatacttttc
    59161 atcatgccta cgctgtcaac gtatctcatc ccggcgctta aaccctggcg ttccatcgga
    59221 aatctgatta cgctgatcgt gggtatcctg tgcgtatcgg taatgttctt tagctaatat
    59281 ccgttgtgga tggttcaaaa ttgctacaat tttgaaccat tccatttttc ataatattca
    59341 tttgttatta ttccgcccta tcacttctct cgtcatatcc tagaattatc agttagttaa
    59401 acacacaaag agatctgaac atcttaaata agactattta agatgcataa cttagattcg
    59461 caagatatct tctggtcatt caaaaacaat ttgcgatata gatattctca taccatgaga
    59521 tagttatctg gaccacgttc gaccatgcaa gattaaatca gtgaaacata atcatattta
    59581 ataacgagaa atgcattttt aaatatctta atcagaatta tagcaaatac agattaatcg
    59641 caatttttcc cagcaaaatt acaaaatcaa ggtgtattga catcatcaat caccaatgaa
    59701 attatgcaat tatatacgga tagggaggtt cttaacatgg ggctttgtag tcgttataaa
    59761 agtcttacat gcaatagttg ctcaatgcat tgccaaataa tgccagaaga gtcaccgcgt
    59821 ttgcagtact gcgcaaactc gtgtt
//
LOCUS       DAEEUE010000012        59810 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.012, whole genome shotgun sequence.
ACCESSION   DAEEUE010000012 DAEEUE010000000
VERSION     DAEEUE010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59810
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.012"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            44..1774
                     /locus_tag="KLK84_000775"
     CDS             44..1774
                     /locus_tag="KLK84_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HBE2008223.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            1771..2685
                     /locus_tag="KLK84_000776"
     CDS             1771..2685
                     /locus_tag="KLK84_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBE2008224.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            2718..2966
                     /locus_tag="KLK84_000777"
     CDS             2718..2966
                     /locus_tag="KLK84_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBE2008225.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            2966..4137
                     /locus_tag="KLK84_000778"
                     /pseudo
     CDS             2966..4137
                     /locus_tag="KLK84_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(4173..5252)
                     /gene="lptG"
                     /locus_tag="KLK84_000779"
     CDS             complement(4173..5252)
                     /gene="lptG"
                     /locus_tag="KLK84_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBE2008226.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(5249..6319)
                     /gene="lptF"
                     /locus_tag="KLK84_000780"
     CDS             complement(5249..6319)
                     /gene="lptF"
                     /locus_tag="KLK84_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBE2008227.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(6350..6910)
                     /locus_tag="KLK84_000781"
     CDS             complement(6350..6910)
                     /locus_tag="KLK84_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBE2008228.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(6922..7746)
                     /locus_tag="KLK84_000782"
     CDS             complement(6922..7746)
                     /locus_tag="KLK84_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2008229.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(7746..9095)
                     /locus_tag="KLK84_000783"
     CDS             complement(7746..9095)
                     /locus_tag="KLK84_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HBE2008230.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(9141..9899)
                     /locus_tag="KLK84_000784"
     CDS             complement(9141..9899)
                     /locus_tag="KLK84_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2008231.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(9931..10644)
                     /locus_tag="KLK84_000785"
     CDS             complement(9931..10644)
                     /locus_tag="KLK84_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBE2008232.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            10818..11510
                     /locus_tag="KLK84_000786"
     CDS             10818..11510
                     /locus_tag="KLK84_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBE2008233.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(11559..13058)
                     /gene="pitB"
                     /locus_tag="KLK84_000787"
     CDS             complement(11559..13058)
                     /gene="pitB"
                     /locus_tag="KLK84_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HBE2008234.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(13350..15209)
                     /gene="gss"
                     /locus_tag="KLK84_000788"
     CDS             complement(13350..15209)
                     /gene="gss"
                     /locus_tag="KLK84_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HBE2008235.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            15414..16280
                     /gene="yghU"
                     /locus_tag="KLK84_000789"
     CDS             15414..16280
                     /gene="yghU"
                     /locus_tag="KLK84_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HBE2008236.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHELHDAS
                     DFETNTEDKRQG"
     gene            complement(16403..16651)
                     /gene="hybG"
                     /locus_tag="KLK84_000790"
     CDS             complement(16403..16651)
                     /gene="hybG"
                     /locus_tag="KLK84_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HBE2008237.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(16785..17126)
                     /gene="hypA"
                     /locus_tag="KLK84_000791"
     CDS             complement(16785..17126)
                     /gene="hypA"
                     /locus_tag="KLK84_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBE2008238.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(17119..17607)
                     /gene="hybE"
                     /locus_tag="KLK84_000792"
     CDS             complement(17119..17607)
                     /gene="hybE"
                     /locus_tag="KLK84_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HBE2008239.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRSE
                     NA"
     gene            complement(17600..18094)
                     /locus_tag="KLK84_000793"
     CDS             complement(17600..18094)
                     /locus_tag="KLK84_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HBE2008240.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(18094..19797)
                     /gene="hybC"
                     /locus_tag="KLK84_000794"
     CDS             complement(18094..19797)
                     /gene="hybC"
                     /locus_tag="KLK84_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HBE2008241.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(19794..20972)
                     /gene="hybB"
                     /locus_tag="KLK84_000795"
     CDS             complement(19794..20972)
                     /gene="hybB"
                     /locus_tag="KLK84_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HBE2008242.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(20962..21948)
                     /gene="hybA"
                     /locus_tag="KLK84_000796"
     CDS             complement(20962..21948)
                     /gene="hybA"
                     /locus_tag="KLK84_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HBE2008243.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(21951..23069)
                     /gene="hybO"
                     /locus_tag="KLK84_000797"
     CDS             complement(21951..23069)
                     /gene="hybO"
                     /locus_tag="KLK84_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HBE2008244.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(23258..23545)
                     /locus_tag="KLK84_000798"
     CDS             complement(23258..23545)
                     /locus_tag="KLK84_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HBE2008245.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYCLEN"
     gene            complement(23664..24551)
                     /locus_tag="KLK84_000799"
     CDS             complement(23664..24551)
                     /locus_tag="KLK84_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBE2008246.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            24708..25748
                     /gene="gpr"
                     /locus_tag="KLK84_000800"
     CDS             24708..25748
                     /gene="gpr"
                     /locus_tag="KLK84_000800"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HBE2008247.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(25788..26282)
                     /locus_tag="KLK84_000801"
     CDS             complement(25788..26282)
                     /locus_tag="KLK84_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HBE2008248.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            26473..27357
                     /gene="yghA"
                     /locus_tag="KLK84_000802"
     CDS             26473..27357
                     /gene="yghA"
                     /locus_tag="KLK84_000802"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBE2008249.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(27629..28054)
                     /gene="exbD"
                     /locus_tag="KLK84_000803"
     CDS             complement(27629..28054)
                     /gene="exbD"
                     /locus_tag="KLK84_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HBE2008250.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(28061..28795)
                     /gene="exbB"
                     /locus_tag="KLK84_000804"
     CDS             complement(28061..28795)
                     /gene="exbB"
                     /locus_tag="KLK84_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBE2008251.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            29047..30234
                     /gene="metC"
                     /locus_tag="KLK84_000805"
     CDS             29047..30234
                     /gene="metC"
                     /locus_tag="KLK84_000805"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HBE2008252.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            30374..31033
                     /locus_tag="KLK84_000806"
     CDS             30374..31033
                     /locus_tag="KLK84_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBE2008253.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(31073..31972)
                     /gene="yqhC"
                     /locus_tag="KLK84_000807"
     CDS             complement(31073..31972)
                     /gene="yqhC"
                     /locus_tag="KLK84_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HBE2008254.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            32166..33329
                     /gene="yqhD"
                     /locus_tag="KLK84_000808"
     CDS             32166..33329
                     /gene="yqhD"
                     /locus_tag="KLK84_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBE2008255.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            33434..34261
                     /gene="dkgA"
                     /locus_tag="KLK84_000809"
     CDS             33434..34261
                     /gene="dkgA"
                     /locus_tag="KLK84_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HBE2008256.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            34461..35387
                     /locus_tag="KLK84_000810"
     CDS             34461..35387
                     /locus_tag="KLK84_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HBE2008257.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            35438..35695
                     /gene="yqhH"
                     /locus_tag="KLK84_000811"
     CDS             35438..35695
                     /gene="yqhH"
                     /locus_tag="KLK84_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HBE2008258.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(35738..37957)
                     /locus_tag="KLK84_000812"
     CDS             complement(35738..37957)
                     /locus_tag="KLK84_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBE2008259.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVVHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIAPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(38068..39480)
                     /gene="ftsP"
                     /locus_tag="KLK84_000813"
     CDS             complement(38068..39480)
                     /gene="ftsP"
                     /locus_tag="KLK84_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HBE2008260.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(39555..40292)
                     /gene="plsC"
                     /locus_tag="KLK84_000814"
                     /gene_synonym="parF"
     CDS             complement(39555..40292)
                     /gene="plsC"
                     /locus_tag="KLK84_000814"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HBE2008261.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAENYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(40526..42784)
                     /gene="parC"
                     /locus_tag="KLK84_000815"
     CDS             complement(40526..42784)
                     /gene="parC"
                     /locus_tag="KLK84_000815"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBE2008262.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(43330..43812)
                     /locus_tag="KLK84_000816"
     CDS             complement(43330..43812)
                     /locus_tag="KLK84_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBE2008263.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(43865..44257)
                     /gene="ygiW"
                     /locus_tag="KLK84_000817"
     CDS             complement(43865..44257)
                     /gene="ygiW"
                     /locus_tag="KLK84_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HBE2008264.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            44409..45068
                     /gene="qseB"
                     /locus_tag="KLK84_000818"
     CDS             44409..45068
                     /gene="qseB"
                     /locus_tag="KLK84_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HBE2008265.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAAKLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            45065..46339
                     /gene="qseC"
                     /locus_tag="KLK84_000819"
     CDS             45065..46339
                     /gene="qseC"
                     /locus_tag="KLK84_000819"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HBE2008266.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(46385..46717)
                     /locus_tag="KLK84_000820"
     CDS             complement(46385..46717)
                     /locus_tag="KLK84_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HBE2008267.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            47036..47617
                     /gene="mdaB"
                     /locus_tag="KLK84_000821"
     CDS             47036..47617
                     /gene="mdaB"
                     /locus_tag="KLK84_000821"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HBE2008268.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            47648..47962
                     /locus_tag="KLK84_000822"
     CDS             47648..47962
                     /locus_tag="KLK84_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBE2008269.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(48010..49902)
                     /gene="parE"
                     /locus_tag="KLK84_000823"
     CDS             complement(48010..49902)
                     /gene="parE"
                     /locus_tag="KLK84_000823"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBE2008270.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(49931..50512)
                     /gene="yqiA"
                     /locus_tag="KLK84_000824"
     CDS             complement(49931..50512)
                     /gene="yqiA"
                     /locus_tag="KLK84_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HBE2008271.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(50512..51339)
                     /gene="cpdA"
                     /locus_tag="KLK84_000825"
     CDS             complement(50512..51339)
                     /gene="cpdA"
                     /locus_tag="KLK84_000825"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HBE2008272.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(51364..51786)
                     /locus_tag="KLK84_000826"
     CDS             complement(51364..51786)
                     /locus_tag="KLK84_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HBE2008273.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(51787..52416)
                     /gene="nudF"
                     /locus_tag="KLK84_000827"
     CDS             complement(51787..52416)
                     /gene="nudF"
                     /locus_tag="KLK84_000827"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HBE2008274.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            52621..54102
                     /gene="tolC"
                     /locus_tag="KLK84_000828"
     CDS             52621..54102
                     /gene="tolC"
                     /locus_tag="KLK84_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HBE2008275.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            54250..54921
                     /locus_tag="KLK84_000829"
     CDS             54250..54921
                     /locus_tag="KLK84_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HBE2008276.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            54927..56087
                     /locus_tag="KLK84_000830"
     CDS             54927..56087
                     /locus_tag="KLK84_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBE2008277.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLSEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(56125..56913)
                     /gene="ygiD"
                     /locus_tag="KLK84_000831"
     CDS             complement(56125..56913)
                     /gene="ygiD"
                     /locus_tag="KLK84_000831"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HBE2008278.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPVAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            57056..57829
                     /gene="zupT"
                     /locus_tag="KLK84_000832"
     CDS             57056..57829
                     /gene="zupT"
                     /locus_tag="KLK84_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBE2008279.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(57887..58057)
                     /locus_tag="KLK84_000833"
     CDS             complement(57887..58057)
                     /locus_tag="KLK84_000833"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2008280.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(58318..58971)
                     /gene="ribB"
                     /locus_tag="KLK84_000834"
     CDS             complement(58318..58971)
                     /gene="ribB"
                     /locus_tag="KLK84_000834"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HBE2008281.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(59075..59223)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            59345..59635
                     /gene="ubiK"
                     /locus_tag="KLK84_000835"
     CDS             59345..59635
                     /gene="ubiK"
                     /locus_tag="KLK84_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HBE2008282.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT        14968 a        15862 c        14835 g        14145 t
ORIGIN      
        1 ccaccgcatt attgtcattt tatttttgat atccaatttt ttcgtggtgt atatgtctaa
       61 taaaatcttt acgcattccc tacctatgcg ctatgccgat tttccaacgc tggttgatgc
      121 tttggactac gccgctctga gtagagccgg aatgaatttt tatgacagac gttgccaact
      181 tgaagatcaa ctggaatatc agacattaaa aacgcgtgcc gaagctggtg cgaagcggtt
      241 gttatcgctg aacctgaaaa aaggcgaacg cgtggcgctg attgccgaaa cgagtagcgg
      301 gtttgtagag gctttttttg cctgtcagta tgctggctta gtcgccgtcc cgttggcgat
      361 tccaatgggc gtcggtcagc gggattcctg gagcgccaaa ctccagggtt tactggcaag
      421 ttgccagccc gcagccatta tcactggtga tgagtggttg ccactggtca atgccgcgac
      481 gcataacaac cccgaattac atgttttaag ccacgcttgg tttaaggcat taccggaagc
      541 cgatgttgcg ctccagcgtc cagttccgaa cgatatcgcc tacctccagt acacctccgg
      601 cagcacccgt tttccccgtg gcgtcattat cacccatcgc gaagtgatgg ctaatctacg
      661 tgctataagc cacgatggca ttaaattacg ccctagcgac cgctgcgtct cctggcagcc
      721 tttctaccat gatatgggac tggtcggctt tctcctgccc cccgtcgcca cgcaactttc
      781 agtagattat ttgcgcactc aggattttgc catgcgtcct ttgcaatggc ttaaattgat
      841 cagtaaaaat cgcggcaccg tttccgttgc gccgccgttt ggctatgaat tgtgccaacg
      901 ccgcgtgaat gaaaaagatc tcgctgaact ggatctttcc tgctggcgcg tcgctggtat
      961 tggtgctgag ccgatctccg cagaacaact ccatcaattc gctgaatgtt tccgtcaggt
     1021 taactttgac aataaaactt tcatgccgtg ctacggactg gcagaaaatg cgctggctgt
     1081 cagcttctct gatgaagcct ccggggttgt ggttaacgaa gtggatcgcg acatcctcga
     1141 atatcagggt aaagccgtcg cgccgggtgc agagacacgc gccgtatcga ctttcgtcaa
     1201 ctgcggcaaa gcgttgccgg aacatggtat tgaaatccgc aatgaagcag gtatgccggt
     1261 cgcggaacgt gtggtaggcc atatttgcat ctccggtccc agtctgatga gcggttactt
     1321 tggcgaccag gtttcgcaag acgagattgc cgcgacgggc tggttagaca ccggcgacct
     1381 cggttatctg ctggacggtt atctgtatgt caccggacgc attaaagatc tgattattat
     1441 tcgtggccgt aatatctggc cgcaggatat tgaatatata gcggaacagg aaccggaaat
     1501 tcattctggc gatgcgattg cttttgttac cgcccaggaa aaaatcattt tgcagatcca
     1561 gtgtcggatc agcgacgaag aacgtcgcgg gcagcttatc cacgcgctgg cggcacggat
     1621 ccaaagcgaa tttggcgtga ccgcggctat cgatctgttg ccgccccaca gtattccccg
     1681 aacgtcctcc ggcaagcctg cccgtgcgga agcgaaaaaa cgttatcaga aggcttatgc
     1741 tgccagtctt aatgtgcagg aatccctggc ataaatcaaa ctgtcgcggt gacgggcgct
     1801 accgggttta tcggtaaata tattatcgat aacctgctcg cccgcggctt tcacgttcgc
     1861 gcattgacgc gtactgcccg cgctcacgtc aatgataatc tcacctgggt gcgcggttcg
     1921 ctggaagata ctcattcact tagcgagctg gttgccggag ccagcgtagt ggtccattgc
     1981 gccgggcaag tgcgcgggca caaagaagag attttcaccc gctgtaacgt tgacggcagc
     2041 ctgcgcctga tgcaagcagc aaaagagagc ggcttttgcc aacgttttct gtttatctct
     2101 tcgctggcgg cgcgccatcc cgagctctcc tggtatgcaa aatccaaata cgtcgccgag
     2161 caacgactgg cagcaatggc ggacgaaatt acgctgggcg tttttcgccc gacagccgtg
     2221 tatggtcccg gcgataaaga gttaaaaccg ctgtttgact ggatgctacg cggcctgctg
     2281 ccacgacttg gtgcgccaga tacacaactc tcttttcttc acgtcaccga ttttgcgcaa
     2341 gcagtgggtc agtggttaag cgccgaaact gtacagacgc aaacctatga attatgcgat
     2401 ggcgtcgccg gtggctatga ctggcaacgc gtacgacagc ttgccgccaa agcccgttgt
     2461 ggttccgtgc gaatggttga cattcctctg ccggtactca caggccttgc ggatatcagt
     2521 accgcgttga gtcgcctggc aggtaaagaa cctatgctga cccgctcgaa aattcgtgaa
     2581 ttaacccacg ccgactggtc ggcaagtaat aaccgtattt ctgaagatat caattggttt
     2641 cccgggatta gcctggaaca cgcattacgc aacgggctat tttgattgtc ttgttttaaa
     2701 ggtgtgttaa cgtttctatg gtaaatcgtg aaatagtaat ggattacatc ctttcctgtt
     2761 tacaggattt ggtcgaaaac ggtgtggaaa ttaagcccga cagcgatctc gtcaacgatc
     2821 ttggcctgga gtcgatcaaa gtgatggatc tcttaatgat gctggaagac agattcgata
     2881 tttccattcc tattaatatt ctgctggatg tcaaaacccc tgcgcaatta atggaaactc
     2941 tacttccatg gctggagaat aaataatggg gctatacgat aaatatgcgc gccttgctgg
     3001 cgagcgtctg caatttagtg acaacgggtt aacaccgttt ggcacctgta ttgatgaagt
     3061 gtattccgcc accgaaggcc ggatcggcaa taaaaaagtg attctggccg ggaccaataa
     3121 ttatctcggt ttaaccttta atcatgatgc catcagcgaa ggtcaggccg cgttagcggc
     3181 ccaaggcacc ggcaccaccg gttctcgtat ggcaaacggc agttatgcgc cgcacctggc
     3241 gttagagaaa gagattgccg agtttttcaa tctccctacc gctatcgttt ttcaaccggt
     3301 tataccgcta atttaggcgt catcagcgca cttgccgacc ataacgccgt ggtgttactg
     3361 gatgccgaca gccacgccag tatttatgac gcctgttctc tcggtggcgc ggaaattatt
     3421 cgtttccgcc ataacgatgc taaagatctg gaacggcgca tggttcgcct cggtgaacgg
     3481 gcgaaagagg cgattatcat cgttgagggc atctacagca tgctcggtga cgttgcgccg
     3541 ctggcggaaa ttgttgatat caaacgtcgt ctcggtggtt atttgattgt cgatgaagcg
     3601 cattcgtttg gcgtgttagg cgcaacgggg cgcggactgg ctgaagctgt cggcgttgaa
     3661 gatgatgtgg atattatcgt tggtactttc agtaaaagtc tggcctcgat tggcggtttt
     3721 gctgtcggtt cggaagcgat ggaagtgctg cgctacggca gccgcccgta tatttttacc
     3781 gcctctcctt ctccgtcctg cattgcgacc gtgcgctcgt cgttaagaac tattgccaca
     3841 caaccagagc tgcggcaaaa gctgatggat aacgccaacc atctgtatga cggtctgcaa
     3901 aaactgggtt atgagttaag ttcacatatt agcccggtag tgccagtaat aatcggttcg
     3961 aaagaggaag gtttacgcat ctggcgcgag cttatctcgc cgggcgtgta tgtgaatctt
     4021 atcctgccgc ctgccgcgcc tgcaggcatc acgctgctgc gctgtagcgt aaatgctgcc
     4081 cacagtcgtg agcaaatcga tgccatcatt caggcatttg ctactctgaa gcagtaatgt
     4141 gagcccgccg tggtgtttgc gccacggcgg gtttaatgtt gtctgtcata ccaatacacc
     4201 aggctcaacg ccaccaacac aaacgctacc ggtaaaccga gcgcggtaac aggtgcgctt
     4261 aacgcaaata gcaatcccag attaaccatg atttgatagc tgatccaggt gagtaagccc
     4321 acgatgacgc ctacagcgag acggctaccc atcccaggcg aacgcggggc actaaaggta
     4381 aacggcacag ccagcaaaat catcgccagg gtcaggatgg gttgccccag tttttcccac
     4441 agtgcaaggc ggtattcgct gctgggttgc ccggtttcct gcaaataatg gatgtaatga
     4501 ttaagctgac ggacagaaaa agtattcccc ggcattgata actcttcaag attcatactg
     4561 gtgaaggccg attgccaggc aagattatct aatgtttcca gcgtttcttt gccattcagc
     4621 cattttttat gattcacacc atgcaggacc cacgttttgt cgtcttcaat ggtggcactg
     4681 cgtgcataaa tgtaggattc aagcgagaga tcgtcgcgat aatgaaatat ctccacgccc
     4741 accggctggc cctgctcgtt cagcgatttc accgtcacaa attcattacc acgcctggcc
     4801 cacagcatat tgccggtaat gtcatcgtcc tcccccaacg cggtggcggt cgatttgatt
     4861 tgcaacgcct gttgctgcaa tggcgacgcc acccactcat ctatcgcgcc taacgaaaca
     4921 gtccacaata tcccggcaac cagcgccacc agtgcaatac ggaagataga aaaccccgtg
     4981 ctgcgaatgg cggtaagctc actgtttttc gataactgcc ccagtccaac aataccaccc
     5041 aataacgcga taaacggcga aagttcgacc agtgtgcgcg gtaaggtcat tagcaccacc
     5101 agcaccgcct gagtccagcg ataaccgccc gggctgacac catccagttc gttaatcagg
     5161 ttgaaggtgg taaaaagcgg cagcaatagc cctgcagcgg cggcaaaacc gagaaagaga
     5221 tgacggatta aatagcgact gaaaacattc atcgggaaaa tttctgcagc aaagagaagt
     5281 cacgtgcgac cagtatcagc aggcccatta gcatgagtcc gggcactaac cacaaaccag
     5341 gaatgagggg aatcgcgccg ttagccacca gtgtacggca gacgtcgccg ccataaaaaa
     5401 tggcaataaa cagcaacgtc agtggcaata acgttgaaaa tcgcccttgc cgcggtttta
     5461 cccgacttaa tgaaatggcc agcagcgcca tcaacaatgc ggtcaggcca cggctttgtc
     5521 gccattgcag ttcggcatga tcggcaggaa aggcggagcg cgccagctcc gtgaccgatt
     5581 tcgctttgcg tttgacgttc gggctttgat ccagcggatt cagatgtaat tgcagattac
     5641 ggtagatctg ttcgttgtcg tcacgcccct gatgatcgag aagataggca gtgccgttgt
     5701 gcaacataac ggtcggtttc tcgggtgatg ggtcaaccac atcaaccgaa ccggcgcgga
     5761 aaatacgggt tcgattggca gtagaagtat aaatcagcgc gtcagtcagg cgattattat
     5821 cctgatcaac cgtctgcgaa aggatcattc gtccgttatc gttagtgtta aatttctttg
     5881 cccgcaactg gcgaacatcc agctccgact gcgactgctg ctccagttga taaatctgcg
     5941 cataagccca cggacgacca tacatcgaca gaagggtgac aaaaatactt agcggtatcg
     6001 ccagataaag aacggctttg tacaagcgtc ccggactgcc ccccgccgca gagatagcgg
     6061 taatttccga gtcggtatac atctgcccta gcgtcacgcc aactgacaca tacagaccaa
     6121 caggtaacaa catctccagt gcaatcagta ctttgtagaa aacgatatcc agcacaacat
     6181 caagagctaa tgtcccattt gccgcttcgg tcagataacg ctgtgcggag tagctggcga
     6241 aaataaagat cagaaaaccg acgataatca gcaccagacg gcgcgtacca cgcatgatgt
     6301 agtgttcaac cagcttcatc tcactaacgc cctcaatcct ttgagttttt tattcattga
     6361 tcagtttctg cgccaacgca atgacatgat ctacctcttc cggcgtcagg ctgccgtcct
     6421 ttgcccactg aatttgcccg gctttattca gcaccagaat ggttgaactc tgctccggca
     6481 agcgccaggc aacgcgtccc tgtccattgc cgtcaataac gaattgcgcc cacggatagc
     6541 ggcgtttatt tttttcgatt ttgccaacaa caaaatagcc agtaccaaag atggcatcat
     6601 cggtgttcac aatggtggtg ggctgaaaac gatcctgcgg aaagtttgcc gcttcaaccg
     6661 ctttaattaa cagtgagttc tttttcttcg ccgacttacg cccggcgata tactgaataa
     6721 tgcggacttt tcccgctaat tgtgtgctgt tccacttttg gtagtgaatt tcgtcgtcgc
     6781 tattcagaag cagctcgccg ccttcctgga tataaacagg ggtaaccgtt ttgccagtga
     6841 cgaaattgtg tgcctgcaca cccggggcta agagcatgat aatcaacgca ataatcaaac
     6901 gagatgacat cattgctccg gctatagcgg aaaacgcgaa atcagcagct ctaagcgctg
     6961 acaaatatcg ctatccagcg ccaacggtgc ataatacgac tggcggtttt ccatatcatc
     7021 cgccaccatg tcgtcgtgtt tatctcccat caggccatta atggcggcga ggaaacggga
     7081 aatctgtaac gcctctttat tttcaggatc aagcgatctt aaggtgtcgt aataagaaac
     7141 ctgctggctg atgatttttc gcacatcatg aaattccctg ccggttagtc gcggtttggc
     7201 aataatctct ttcagggtat gcatttgctt ttgccgatag gcaataaaac tgcttacgct
     7261 ttctaactgg ctatggcgta atgaatagtt caccaacgac cagaccggag agcttaaata
     7321 aatccgtaac aggttgccgt aatacgacac aatatttttc ttaccgttac gaaagccgtc
     7381 ctgaaaacgc cccagaaaaa cggtcgtttt gccgaataaa aatccggcct gatgcttctt
     7441 taaatacagc cgttgcgcaa aacgcaggtg cttgtagcgc gctcgcatgt atttaaattg
     7501 tttgcgctca tcagcagtca gctcatcgcc tttggcttgt ttgcgaacta aacgcagtaa
     7561 ttcctgacga tcaacgccat tgagccagaa ttgcaggcat aacgcgtaat tatcatgaat
     7621 gatttcggtg gtacaactta gtgcaatagt cggcggcaaa ctaacctgtg catcaacgac
     7681 atcatcttct tcaatcgcaa caaatagacg ctcgctcgcc tcaagcggga aatattttct
     7741 catcatcagg atttctctcc ggctgcgtta taaagcaagg aatggcgctg aaaaaatgcc
     7801 tttaaggttg gaattaacga gagcgccacg tccagacatt gcccaccgaa gtaagcaaac
     7861 gctgctccca gcacaccaaa cagatgacag aacatgaaca gcaatacgat gtagccgatt
     7921 gaggcgatgg tttgcgccac gagaaaagcg cgctgttttc ccgccatcaa tagcaatgac
     7981 tcctgcggga agcccagcat cgaaatcaca attgcgccca gcatcacctg aatcaggtca
     8041 tacgcttcga gatacttaac gccaaacacc agcgaaatga gcggcttgcc gacgatcaac
     8101 accgcgagag ccaccagaat accgattccg cccgccagta aaccggactt cacacctaac
     8161 aaccacggtc tggtggtgcg cggatctaaa cgcatcacct ctgggtaaaa acttttaccc
     8221 agcaaacccg ctggcgttcc ggcggcgtcg aagaatgtca tggcgatttt aaataacccg
     8281 gcggcagcgg gtcctaacac gatccccacc aacactgtgc tgcacgagtt acgcgccgac
     8341 cagatggagt gggcaatgtt ggttgaccag acaaaactcc acgcgccttt aatgtgtcgg
     8401 gcagactcaa acagattcaa tttgaaggcg ttatggatat ttctacggcg taattcgcgc
     8461 gcggcaaacc accagtacat ggtgccgcca accagattcg acacgtacca ggcaataaca
     8521 aaacccgcaa aaccaaagtc aaaataccag gctgcgacgc tccccgctgc gcgcagaaaa
     8581 ggtttcgtcg cctgctgtac agcaatcaaa tcaaagcgat ctaccgcacg cagaatgccg
     8641 gtcggcgtgg aggaagccat tgaaggaatg agcgtgcaat acagcgccgc cagccaaaag
     8701 ctttggtcat ccagacctaa tgaatgggaa aggaatggca gtaaggcaat gccgccgaca
     8761 atcgccaccg cgccgctgac gatatccagc gagaaggaaa atgagacgac attgcgaaat
     8821 tgctgcggat tattgttggt caaggctggc gttccgtact gaaccaccag ctgccatgtc
     8881 tgaaacttaa taaaatcgct gatcgacttg gcgtacgatt gcacaatcac cagtacacca
     8941 aacatggcgg gcgtcatccc tttaccggca cacgagagcg ccaacagccc cagcaaggcg
     9001 ctctcgacat tactggagcc taaccaggcg ctattgcgaa taatggtgcg aaacgcgcca
     9061 tctgcaaacc aatgtttgat gttaaaaccc gccaattcag cctgacctta tcgttgatag
     9121 taaaaaatat cccgccagcc ttaagttaaa cttcggcggt cagaaacgat ggcaaccaga
     9181 gaaaccgcct tctgtacctg ttccagcact tcgctgtagg gcgctctgga atcaatctca
     9241 agaatttttg tgccgttata gccaatcgtc gacatgacgc cgattttgtc ctgcagctcg
     9301 gcatactcat ggtcaggctt gcgggagatg gcagtatcaa tatcaatccc caaacgaatg
     9361 attaattcag gacggtattg cgccattttt tggtataaac tccgttcgcg ctgcgccaga
     9421 aacatgctga ttttcccggt cgcacgttcg acgccgatcc ccggtccgtc ataataaaag
     9481 cccgaaattt cagcctgcgg gaagcgatcg ctgaccacca gaacgccact ttgcgccagt
     9541 cgctgaacct tgcgtagatt cgccattctt cgcagcgaga agcagtacat aatcaccgcc
     9601 gcccatagcg ccggagattt ggttttcatg ctttgggttt tcgaggattt ggccgccaat
     9661 cgccgttcca gccagacgcc aaccaacggc aatcgtttga ttttgtcgcc gtcttcgccg
     9721 gagagcagcc ccagatagcg gcgctcggtt tgccagtgtt gttgcagcga tttcaccagg
     9781 tcggtggtca gcgtggattt accggtgcca tcacacccga ccaccgcaat cagccccgga
     9841 atgtagttgg gctgcggcgc ggttgtacta ttaacagttt gtgtttgtag tgcatccatt
     9901 cgttaaatca tcccctatcc attttggtga tcaggaaagc gcggcgtgaa ttgcgcgtag
     9961 tgacgcttgc ataatttcat cggctggttg ccgaccatcc agttcaagga tcttggcgcc
    10021 attgaatgtc agttgcggcg taacggcgat tttttcctgt aacgctgcca gttggtggtc
    10081 aggtttacgc gcaaacgcgg tttgttcatc aatgccaaga cgaatcaaca atacgggtaa
    10141 ataagacgcc atccattggt acagcttcaa ctcgcgctgc cttaacattt ttatccaacc
    10201 gttaccgccc gtggtttttg ccaattgcgg gccatcaaag cgaaaacccg gcacttcaac
    10261 ctgcgggtag cggtcggtga tgagcagata gccttgctgg cttttacaca acattttgcg
    10321 aaacttgtac gcccgccagc aggaaagcag atagataacc agcgcagtaa tattgccagg
    10381 cggagttaag ggcttttcgt gtacatgtgc cgctttactt cgcagataac acccacaagg
    10441 tgcgccaata acagggagct gtgaaatcca ttcgccaatt cgcccggacg attgcccgag
    10501 ataaatgtgt tctgttggca ttcttgctgc cagttcattt accaggcttg ccgtgagggt
    10561 cgatttacct gaaccatcac atcctacaat ggcaataaca cgcactggcg tggaattaat
    10621 tattgacata ttacgttgat tcacgaaaaa cccggcagta aataatgtat tgaatattac
    10681 gtggtccgtg ctcagaatat ccgttcaacc ttattgtgac aatggctaat tgtgacattg
    10741 acctgaatta cgctttcata aaaacatatt aaccaaataa atatttttaa tggatattta
    10801 aattaaagga tatattcatg cagtcaataa cacctccatt aattgccgtt attggtagcg
    10861 atggttcagg caagtcaacg gtgtgtgaac atcttattac cgttgtcgaa aaatatggtg
    10921 ctgccgaaag agttcattta ggaaaacagg ccggaaatgt cggtcgtgca gtgacaaaat
    10981 tacctttgat gggaaaatcc ttacataaaa caattgaacg aaatcaggtg aaaacagcaa
    11041 aaaaattgcc tggaccagtt ccggcgctgg taattacagc gtttgtcgcc cgtcgcttac
    11101 tgcgctttcg tcatatgctt gcctgtcgtc gtcgcgggtt aattgttcta accgaccgtt
    11161 atcctcagga ccaaattcct ggcgcttacg atggtacggt gttcccacct aacgttgaag
    11221 gtggtcgttt tgtctcatgg ctggcaagcc aggaacgtaa agcgtttcac tggatggcga
    11281 gccataagcc tgatctggtc atcaaactca atgttgacct tgaagttgcc tgtgcacgta
    11341 aacccgacca taaacgggaa tcgctggcga ggaagattgc cataacgcca cagttaacct
    11401 ttggtggtgc acaactggtt gatatcgatg ccaatcagcc actggaacag gtgttggttg
    11461 atgcagaaaa agcgattacg gattttatga ccgcgcgtgg ttatcactag tcaaaaatgg
    11521 aaatgcccga tcgccaggac cgggcatttt caggaaggtt aaatcaactg caatgctatc
    11581 cagtacagcc caccagaaag aaaaattgcc gccggtaaag taaacaccca cgccatcagg
    11641 atgctggtta ccgttttacg ctgtaaccca ccgccgtcca ccaccatcgt ccctgctacc
    11701 gcagacgaga ggacgtgtgt tgtggagaca ggcatcccaa tataactggc aaggccgata
    11761 gacactgccg ccgtcatttg tgccgccatg ccttgcgcat acgtcatgcc gcgcttacca
    11821 atcttctcac cgatggtcat cgctacacga cgccagccaa tcatggtgcc aatgccgagc
    11881 gccagtgcta ccgccatgat gatccacacc ggagcgtact caatggtgct taacatatcg
    11941 ctgcgcagtt ttttcagcag gttctggtct tctttactga cgcctggcag tttcgccagc
    12001 ttcgcggagg tatcggagat gcacagcata atgcggcgca gctggctgcg ctgactcacg
    12061 cttaacggct cgtaactttc catattgcct ggcagcatcg ttttaacgcg cgcaatcgca
    12121 tcaaaggtat ttgccggatg acagtgaaac tctgttactt gcgtgccatc agtcgatgct
    12181 gcaggcaatg gaggttccat cgcaatcaac ttctgcggca gttcaggatg ctgttgcagg
    12241 tagtgttcga agttggtaac ggcatcgcgg gtacgggtaa tttcatagcc ggacgcattc
    12301 atattgacga cgaagccagc aggggcaatc cccaccagta ccagcattac caggccgatc
    12361 cctttctgcc cgtcgttcgc gccgtgcgaa aacgccacgc ccgcagcgga aacaatcagc
    12421 gcaatacgcg tccagaatgg cggtttacgt ttgccctttt tctttttgcg atcttccgga
    12481 atgcggtgaa tacggtcacg ctttttcgtc ccactccaga aacggcgcag caggaaaatc
    12541 agaccacccg caatgaccag gccgacgata ggggaaacaa tcagcgagga gaaaattttg
    12601 gttacttcac gcaggtttaa cgcatccatc accgatgagc cggttaacag cgcgttggtt
    12661 aaaccgatgc cgataatcgc accaatcaag gtgtgcgaac tggaggccgg taaaccaaag
    12721 aaccacgttc ccaggttcca gataatcgcc gccagcagca tggaaaagac catcgccaaa
    12781 ccgtgagttg accccatatt cagcaacaaa tcgattggta acatatggac aatggcatag
    12841 gcaacgctaa gtccgcccaa taacacgcca aaaaagttaa aaaatgccgc catcaccaca
    12901 gcaagttgtg gctgcatggc acgagtataa ataacggttg ccaccgcatt cgccgtgtca
    12961 tgaaaaccat tgattgcttc gtagaacaac acaaatgcca gagcaagtaa taacaaaagc
    13021 cccgtatata tatcaaggcc aacaaataaa tttagcatat attagattac gccattttga
    13081 atttacgaac ggacgcatta tcagtgactt taacggcatg ggcaaagtga aatatcattt
    13141 tttaacgata aaaagagtgt tatttcttgt gtatcagaca ataagcattc attcaaaacc
    13201 tttagaaata atcaaaacac aggtattcca ctggttaata tattcacata tgaaatgaat
    13261 aaatattgga atatataaat attgaatatt ttgattaatc cccgtactaa ttattcttca
    13321 taatcagtac ggggttgcta caacatgtat tactttttca ccacaattaa cggttcaata
    13381 tcactctctt ttttgatgac cagtgattca tcaccgcgca aacacgtccc accgtagttg
    13441 ccgccaacgg tgaaggtaca tacctgaatg tatttaccgt ccactttcgg caaacaccac
    13501 agttgctgat agatgttttt ctgctcggca aatttaccgc tggttttgtc cagcacctct
    13561 tcatgatggc tgaccaggtc gatattgcta ccacagcgac cggcgatcgg tttcactgcg
    13621 taacctgttt tcaccaattc atcattaaca gtgaaatcgg tatccagcag gtaacgatgg
    13681 tgcgggaaca gcgaccagag gatcggcaga atcgctttgt tgccggggat caccgtccac
    13741 agcggctcaa agaccagcac ttccgggcgc agcaatacgt cgataagacg cacttcgttt
    13801 tgcggatgac cggtacggat tggcaccgca gcaaactcac ggtcgctaac ttcacgaatc
    13861 tgatcaaacg cggtttccca cgcccaggtt ttccacacgc agttaaccag tcgcccttcc
    13921 ccatcaatca gttgcccggc agcatcccag cccagttcat ccagcccgcg caagatacgc
    13981 gtttcaaagc ccgcctggtg cagcgcctgc tccataaact gcgcgtgata gttttcctcg
    14041 atatctttgt cctgcatgat atggacaaac ggacgtgcac gactgtgttt ccaggcacca
    14101 gccagttcgt taatcagccc ttccgccgga ttgaagccgt tgcctttata gccctgctcc
    14161 gcccaacgtt cgaggatcaa gcccgcttcg gtatgacagg aggcggagtc ggcgttgtat
    14221 tcgtaaacct tcaggccacg ctcatccatg cagaaatcca tacgaccagt gatcatatgg
    14281 tgacggcgac gctgccagga gagacgcaaa cgtggccaga ggattttcgg aatgtcgaac
    14341 agcgccagca ggttgtcatc tttcagcacc ttgtcggttg cgtgaagata catcagatgc
    14401 agctcgttgg tggctttaat tagctcctgc tcggcactct cggtaatggt gtagtaatga
    14461 tacggatcct gattgatcac ctgaccgttg gcctgcacat aggcgttttg tagcggatct
    14521 ttttcatcca gccatttacc gtcaaactgg cctttgtttt ccagacgcgc tccactgatt
    14581 ttcagcagct cgcctgcaat ttccggctgc ggtaagctgt attcagtatc ttccgtctgg
    14641 atcatccagc ccagaatggt ggtgtcatca aaagtgtctt taagggtata gcagccgttt
    14701 tcgaccacca tctccagctc gcgcgtccac tgttgccctt gcggcaacgg ggaatgaatc
    14761 acgttctgtt ccgcaatacg gactttgttg ccatgcaatt gggtaatgat ggcgacatgg
    14821 ccagtgtctt taaattcacc gcctttatcc cagataagaa gcgcacccgc gaccggcgca
    14881 cgcggcgagc cgttaggaaa tgcctgcaat ggcaggatgt tgtcattaac cacttctcgc
    14941 aggaagcgca gcgagaaaat ctcccacgcc atacccacgt cagtaaagac cacaccgtaa
    15001 ttcagaaaga gaaaacggcg agcaaattca acgcattgcc acttgtggcc catatattcg
    15061 tcgtcgatat agctacggaa tacggcgtca tcttcgtatt cctgcggatc gagagaactg
    15121 taatctgaag agtagattgc taccccacct ggggcgtagc ccaataatgt cccgaacggg
    15181 gcatcctggc tggtcgttcc tttgctcatc actttacctt taacaataca acctaagcag
    15241 ttggcgtaat tgttgtgctc tgattacctg ctcagcgaca ttaaccggac agaggtcaga
    15301 ctaatcatac acctcatcgc agcggctggc gcaaagggtt aaaaaattca cattctgtac
    15361 agcaagtgac ctgctacact gcttcaacac taccactcag aaggcaactc actatgacag
    15421 acaatactta tcagcccgcg aaagtctgga cgtgggataa atccgctggc ggcgcgttcg
    15481 ccaatatcaa tcgcccgttt tctggtccga cgcatgaaaa aacgctgccc gttggcaaac
    15541 atccattgca actttattcg ctgggaacgc cgaacggtca gaaagtaacg attatgcttg
    15601 aggagctact ggcgctgggc gttactggtg cagagtacga cgcctggctg attcgtattg
    15661 gcgatggcga tcaattctcc agcggctttg tcgaagtgaa cccaaactcg aagatcccgg
    15721 cgctgcgcga tcatacgcat aatccgccga tccgcgtgtt tgaatctggt tcgatcctgc
    15781 tttatctggc ggagaaattt ggctacttcc tgccgcagga tctggcaaaa cggactgaaa
    15841 cgttgaactg gctgttctgg ttacagggtg ccgcgccgtt cctcggcggt ggttttggtc
    15901 acttttacca ttacgcgccg gtgaaaattg agtacgccat caaccgcttt accatggaag
    15961 ccaagcgcct gctcgacgtg ctggataagc aactggcgca gcataagttt gttgcgggcg
    16021 atgagtacac cattgcggat atggcgattt ggccgtggtt tggcaacgtg gtgctcggcg
    16081 gcgtgtatga tgccgcagag tttctggatg cgggaagtta taaacatgtg caacgctggg
    16141 cgaaagaagt gggcgaacgt ccggcagtga aacgtgggcg tattgttaac cgcaccaacg
    16201 ggccgctgaa cgagcagttg catgagctcc atgacgccag tgattttgag acaaatacag
    16261 aagataagcg tcaggggtaa gggttggtgt tcgtcgcagc aagccatcca ggccggataa
    16321 ggcgttcacg ccgcatccgg caatcgtgca caatgcctga tgcgacgctg ccgcgtctta
    16381 tcaggcctac aaaagctaat catcacgcgc tggtaatgtc gtaatccatt tggcgcagtg
    16441 cgtctaatgt ggctttggct tcatcttcgt cgatgatgct catggcaaat ccgacgtgta
    16501 ccagcaccca ctggcccagc agatcggcag ggttaccttc gcaaatcagg gcgatattca
    16561 cgtcgcgctt gataccacat acttcaacct gcgcaagctg gtgaatatct tcaccgacag
    16621 ccagcacctg gcctggaacg ccaatacaca ttgttaactc cggttatttc gattgccgga
    16681 caaacgcgaa aaagaacctt tagcgggtgt tccctctcac cctaaccctc tccccggagg
    16741 ggcgagggga ccgtccgtgc gctttttagg cgttgtcctc agtcttattc aacttcaata
    16801 cttttgacga tcagcgaatc gccggtatcg acacgcaacc gctcgccgtg acagagcgga
    16861 cactgcgcat cgtgctgatg aatctccacc acctggctgc aatcccagca ccaggcctgg
    16921 gcgggtttat agacgatatg taaatcgcac ccttgcgcca ccgttccgtg gcagacaatt
    16981 tcaaaactaa aacggacggc gctctcctca acgcaggaga gcgcgccaat ttccagccac
    17041 acggcggtga cgcgcttaac atcgtgctgc tccgcctgcc gttggataat ttcaacggcg
    17101 ctctggcaaa gcgacaactc atgcattttc gctcctcctg cggccaaaca gcagggcgcg
    17161 acgccctgcg tgtggtacat ccggattggt gactggcaac gaaaggatca ttcgtgcgca
    17221 gtcatccgtc aggcgttggc cttcttcaat cgacatgctg tgcgaaagcg gcgacatcag
    17281 cgaacaggag aggtattgcg aaacaccgtc cagttcgcca acggtaaagg tcatggtgcc
    17341 atacggcagt tgcagaccaa ttttttcact cactttgcgc agcggccaga gttgatccgg
    17401 gccggggaag ataactgcac tcagcatcca cggggtgatc acacaccccg tccactgacc
    17461 ttcgaacagc gtaaaatcag aaacatacac cggcattgaa ggatgcagaa aagagagatc
    17521 gtgcatcgaa cgccgggcaa tttcttcaaa cgctgcctgt acttgcgcct tcggggaggt
    17581 ctggaaacct gctatctctt cagtcatgaa tcgcctcccg tgggatggct tccacgcctg
    17641 attcgcgcag cgcagccaga acctgctcaa gcgcaggttc aatcattgct tcaaccgtcg
    17701 gcgttaagcc gatgtgtggc tccagcgatt ccgggatcac gccgaccagg gtcagctttt
    17761 tcggaaactc accggtgaag cgcagggccg acaatacgtc ggccaggcca agctgatgcg
    17821 gagagatttt gttggtaaac aacgccggaa cttcttcatc ccgcaggatc atcatcgttc
    17881 ccggagtgtt ctttttcgac acaatggcat ccgcaataat cagatgatcg cgatttgcca
    17941 tgtcgccaag cagctccatt cccgccgtgc cgccatcgag gatctcaaca taatccggca
    18001 gaatgtatcg ttgctctaac gcttcgacaa tccgcacgcc gatggcttca tcggtcagca
    18061 aaatattgcc gacccctaag actaaaatac gcattacaga accttcactg aaaccacttc
    18121 gttgccgtcg gcatccacta cgtgtaccgc acaggccatg cacgggtcga aggagtgaat
    18181 ggtacgcacc acttccagcg gtttattcgg atcggcaacc ggtgtaccca ccagcgactg
    18241 ctcgtaagga ccgacgtcat cattgaagtt acgcggacca gagttccagg ttgatggaac
    18301 aaccgcctgg tagttgctga tgataccgtc tttaataacc atccagtgag agagcatacc
    18361 gcgcggcgct tcgaggaagc caacaccttt gaactcaccc gttgccggaa tgttcggctt
    18421 cacaaaggtg gtgtgatcgc ctttgccgat attggtgatc agtgcactgt attggttttg
    18481 caggatatcc tgcaattcac agcagtgaac ggtacgacca ataatacggc ccagcgtgga
    18541 gtgcagctgt gccacttcca gcgtgttgcc agtcagtttc tgataaatcg caacgatttc
    18601 attcagtttg ttctgggtag attcgcgacc tgccgccagt ttcaccagca tattagccag
    18661 cggacccact tctaccgttt tgccgtagaa agtcggtgat ttcacccagg aatatttccc
    18721 gtcgtcagac caaccatcat aagccggaat ggtggtacct tcccacggcg cctgtggcgc
    18781 ttcgtcttta taccaggagt gcttcgcgct ttcctgaatg cctttgatca ggtattcatc
    18841 ggaatgagaa gtgatcggac gatacgagga cagatccgca ttctcaatgt agcctcccgg
    18901 gaacaggaag ctgccgtttt tgctgtcggt cgggaattcc ggcacgctca ggtagttcac
    18961 cgcaccttta ccgcgcgtca gccattccgg gtagaacgcg gcgataaccg cggtatcaac
    19021 cttataaacc tgctcaacaa agtcgctcag tttgtcgatg aaagacttga tgtacatcag
    19081 gcgctcaagg ttcaacacgc ccaacccgtc gaggttgatt gggttcgcga caccacccac
    19141 tgccaggttc tgaatgtgcg gcgttttacc gcccagcagc gccacgacgc ggttagcgtc
    19201 acgctggcac tccagcgcct gcaggtagtg cgctaccgca atcaggttca cttccggcgg
    19261 cagtttcatc gccgggtgac cccagtagcc attggcgaaa atacccaact gaccgctggc
    19321 aaccagatct ttgatcttgt tctgaacttt agtgaactct tccggactgt tcagatgcca
    19381 ggtcgaaacg cctttcagca tttcggaggc tttggttggg tcagcttgca gtgcagaagt
    19441 gatgtccacc cagtccagcg ccgaaagctg atagaaatga acgatatggt catgcgtggt
    19501 gtgcgcagcc agaatgatgt tacggatgta ttgcgcgtta accggaacgt cgatattcag
    19561 cgcactttct gccgcacgaa cggaagacag cgcgtgagtg gtagtacata cgccacagat
    19621 acgttgcaca atcatccatg catcgcgcgg atcgcggttt ttcacgatct cttccatgcc
    19681 gcgccacatg gtaccggaag cccatgcttt cgaaacgacg ccattttcga tttcgcaatc
    19741 gatgcgtaaa tgcccctcaa tacgggttac cggatcaata gtaattctct ggctcatgct
    19801 ttgctcgcct catgacgatt atgatcgttt tgttttaaag gaggaagtat cggcagtaga
    19861 cgaatgagta cgatgtaagc gcaaatctca atagccacaa aaccaataga aatcaacagt
    19921 tcttcccagg tcgggaagta ggcgtaaccg ccgcccgggt tgaatgccac caacgaatag
    19981 gtcagacgcc aggttgcaca acctaacagt gcgctcagtg ctgacaggaa cagcatgcgg
    20041 gaatcattac gcagcttcgc cacacgcaga acgaccagcg ggaagagcat cagcaggact
    20101 tcaatccaga acatcacgga gtagaagtca ccggcaaacg ctaacgacag cttgtcgcga
    20161 tagataagct cgccaaagcg cagcccgatg aaaatcgcca gcaacacact gatggtgttg
    20221 gtcagcttga cgaacaggct cttttcatcc ggaccgttgc cacgcagacc cgcctgcacc
    20281 agtgaacctt caaagatgac aatcgagaag cccatgatga acgccgtcag cagcgagaac
    20341 agcggcaaca tttcatagct ctgccagagc ggatgcacct tgtagcccgc cgagatcatc
    20401 agcgacccca ttgaagactg gtgcatggtc ggcagcagcg caccgagcgc gatgatgaag
    20461 aacatcacct tgttcagacg ttgtagcgac accttccagc ccagacgttc aaacagtgcc
    20521 ggagcaaact ccagagccat cacgccgatg tagatggtca tacagaccgc cgtctcgaac
    20581 agtaccgagt tcacgttgaa gtgacccgga atgtagaagt acggcaggtt ccagtagcga
    20641 cccacgtcga tagtgatcga caagccaccc agtgagtaac caaacagact cgccaacagc
    20701 gccggacgca ccagcggatg gtattgccca cggttaaaga cgtataccgc ccacgccagc
    20761 gcccagccgc cacaggcaaa gccggtgcca atcagcaggt caaacgcgat ccacacgccc
    20821 cacgggaagc cgccgttcag gtcagagaca gagcccagac cgaacaccag acgcttcaca
    20881 atcaggagca tacagatgac gattaacggt ccaaaaatca tgaccggttt actgatgatt
    20941 ttgccgccca gcggttgtgg atcatgactc atgatcgtct cctccgtcgt gatggtcgtt
    21001 tttggtgttg cgacgaacca gcacggttaa gcccgccagc acagccagtg gtagcatcat
    21061 gcctttatac agggtgtgtt gaatattttc ggaacgcgca ccggtagaaa gatcgtccag
    21121 tttcggcagg tcgagatttt cataaggcac acccgtcagt accagaacct gagtaccgcc
    21181 gccctctttc tcgccgtaca gatgcggata atatttcggc accgtatgca ggtaagtgtc
    21241 gccagatttc agcgtctgac gcggatagtg gtattcgctg ccaggcttca gcgccagacg
    21301 ttttttcgcc tccgccatca gctcttcacg cgtaccgaaa atcaccgcgc ccgccgggca
    21361 cacttctacg cagccaggca gaccgccttt atcgagacgt tccacacctt tctggttgca
    21421 cagctcgcac ttatgcagcg caccaaacgg gttgttgtag tcgtacttcg gcacgttgta
    21481 cggacaggcg accatgcagt aacggcaacc ggtgcacacg tctttgtcgt aatggacaat
    21541 gccggttttc ggatcttttt tcagtgcaga gaccgggcac acagagacac agttcggatc
    21601 gacgcagtgc atacactgtt tcttaatgta cgcgtagccg ttctcctcct ggtctttgtt
    21661 gacccctgtg ccgctggtcc acacctgaat gatgttattg gtatacggcg acagtttgtc
    21721 gttgttcgac caggtctgtt ccccttgcgg gttacgttca gggaagttga tatcctgaca
    21781 cttggtgacg caagcctggc agcctacgca caaggtcgag tcgtacaaca tccccagcga
    21841 tcccggaatt ggcgggcggt tttcagcagc cgcatgactg acagacggca gcgcgcccgt
    21901 cagcaatgcc ccgcaggagg ctgctttaat aaaattacgt ctgttcacgg ttattctccc
    21961 cgtgagtcag cgttatcttt cttttgctga cgacccagtt cacgcaccgc catcacgctg
    22021 acaccggcaa ccagcccaac cacaccgccg agcaaaccaa tagcgcctgc agagacgttg
    22081 ccgccctctt tagcgttaac atccggtttc tgtgaacgcg gagtctgatt ttcgacgttg
    22141 gcaagctgat ggatgccttt atggaagccg ataccttttt cgttacagcc atagcacggg
    22201 tgaccaatcg ccaccggcca cacaccgcca acatcgcaga attgcagcgt tgagcagttg
    22261 ccgtaagttt ctggcccttt acagccgagg tggtacaggc accagccttc gcggtggcct
    22321 tcatcaccga actctttggc aaaacgacca gcatcgaagt gcgggcgacg ttcgcagtgt
    22381 tcgtgaatca gacggccata ggcgaaggtc ggacggtttt tgtcatccag tttcggcggt
    22441 ttgccgtaag tgatgatgtg cgcaacggtc gcgaggaagt tgtgcgggtt cggcgggcag
    22501 cccggaatat tgataacggt tttgcctggc agaacttctt gcaagctgac tgcgccagtt
    22561 gggttaactc cagctgcggc aacaccgccc cacgcggagc aggaaccaat agcgataatg
    22621 gctgctgagc cttccgccgc tttgcggatg tgatccacaa tcggctcacc ggcaaccatg
    22681 caataaatac cgttatcttt taatgggatg gaaccatcca ccactaacac atactgccct
    22741 ttgtacttct cgagagcgtt atgtttgttc tcttcgacct gatgaccgaa ggcggcggaa
    22801 agcacttcgt gatattccag agagatagtc tccagcacga ggttttctac cgttggatgc
    22861 gttgcacgaa gcagagattc cgtacaaccg gtgcactcct gcgcgccaat ccagataact
    22921 ggcggacgct gcgggttagt aaccgattcg gccatctctg cagcggcttt gctacttaac
    22981 cccatggtgg cggctaatgc tgcacaaagc ttcatgaaat cacgacggtt aatgccgtga
    23041 gaatggatga gggtgttatc tccagtcatt tatagttatt ccgttgcgag gacctggcat
    23101 atattttgcc tcaatcgcaa aatcaataat gcgatcgatg cgccatttac cacacattta
    23161 ttatggttat cgatatcatg atactgcggc ggggcgctaa aacgaaggga agaagcatta
    23221 gtgtaattaa ttaaagcaag ataatacgta tgtttgatca attttcgagg caataaccgg
    23281 ccataaaaaa gcgcaacgta gaacaggaat tattctcacg aaagaaatcc attaccatct
    23341 ctttatccag tccatagcgg cgcgtcagaa tacccgcttc ccaggcgcta agctggcgat
    23401 ctccggtctt ttcgtacatc cggtgtgagt agcctaatac aaaaccgcgt ttataatcgg
    23461 cacagaattt tgtcacatta accgcactgt cggcatgcgt tgcttttaat cccgccatta
    23521 agcctttacc aaaatggtta ttcatcgttc acctcattcg ttatatatcg ccatatatag
    23581 cgatatttta ggcaaagaac gacttttgcg cacgctattt tttcatactt gtttaagacg
    23641 taatcccgta agagataaac ctatcaggag agatatttat cgaaccattt cagtgtcctt
    23701 tgccaggcaa gatcggcggc agatttgtca taacggggcg tggaatcatt atggaatccg
    23761 tgattaaccc ccggatagat ataagcctca taaaccttat tattgtcttt caacgccgcc
    23821 tcgtaaggag gccagccctc gttgattcgg gtgtccagtt ccgcgaagtg gagtagtaaa
    23881 ggcgcttcaa tcttcgccac atcggcagtg ggtgcctgac gaccataaaa cggcaccgcg
    23941 caggccagtt ccggatacgc gacagccgcc gcgttcgata cgccaccgcc atagcaaaat
    24001 ccggtaatac ccactttacc tgtcgcttgc ggatagcgtt gcataaactc aattgcggca
    24061 aagaaatcat tcatcagttt ggttggatca acctgctgtt gcagctcacg acctttatca
    24121 tcatttcccg gataacctcc aacggaactt aatccgtcag gtgccagggc gatatacccc
    24181 gccttcgcca ctcgccgtgc cacatcttcg atatacggat tcagtccacg attctcatgc
    24241 accaccccca cggctggcgt tttgccgctc atttttgcgg gtttcaccag ataaccccgt
    24301 acctcgccgt gaccatttgg cgaaggatac gtgatgtact cagcaacaat ctccgggtcg
    24361 gtaaactcta cctgagtcgc cagcgcataa tttggcttga gcaaatcaaa caacgctaat
    24421 gccgtcatcc cgcccaccgc atacttcgcc gcaaggttga ggaactcacg tttcgaaatt
    24481 ttcccgtgaa cgtaatagtc gtagtaatcc aacaactctt gtgggaaatc tttggcggtt
    24541 aaacgcggca tcgttgcatt cctcaactgg tgttttttta agcaaagcat aagcgcgtgt
    24601 ttttgcccag tttttcgtca ctctgtgagc cagactacgg gatacgcgct ggcgaatcgc
    24661 taaactagaa acattgtttc gaaattgaac ggaggaaagg agaggtcatg gtctggttag
    24721 cgaatcccga acgttacggg cagatgcagt accgctattg cggaaaaagt ggtttacgcc
    24781 tgcccgcatt atcgctcggt ttatggcaca atttcggtca cgttaacgcg ctggaatcac
    24841 agcgtgcaat cctgcgtaaa gcgtttgatt taggcattac gcactttgat ttagccaaca
    24901 attacgggcc gcctccagga agcgcagaag agaactttgg tcgcctgctg cgcgaggatt
    24961 ttgctgctta tcgcgatgaa ctgattatct ctaccaaggc tggctacgat atgtggcccg
    25021 gcccgtacgg ctctggcggt tcacgtaaat acctgctcgc cagcctcgac caaagcctga
    25081 agcgtatggg gcttgagtat gtcgatatct tttactctca tcgcgtcgat gaaaatacgc
    25141 cgatggaaga aaccgcctct gcgctggctc atgcggtaca aagcggtaag gcgctgtatg
    25201 tcgggatctc ctcttactcg ccagagcgga cgcaaaaaat ggtcgagttg ctgcacgagt
    25261 ggaaaattcc gcttttaatt catcaacctt cgtacaattt actgaaccgc tgggtggata
    25321 aaagcggcct gctggatacc ctgcaaaata acggcgtggg ctgtattgcc tttactcctc
    25381 tggctcaggg attgctgacc ggaaaatatc tcaacggcat tccgcaagat tcacggatgc
    25441 atcgtgaagg gaataaagtt cgtggtctga cgccgaaaat gcttaccgaa gccaacctca
    25501 acagcctgcg cttattgaat gaaatggcac agcagcgtgg acaatcaatg gcgcaaatgg
    25561 cgttaagctg gttgctgaaa gatgatcgcg tgacgtcggt attgattggt gccagccgcg
    25621 cggagcaact agaggagaac gtgcaggcgc tgaataatct gacatttagc accgaggagc
    25681 tggcgcagat tgatcagcat atcgccgatg gcgagctgaa tctgtggcag gcgtcgtccg
    25741 ataaatgacc tgttaataac gggcgcgcga accgcgcccg aatcagatca gtgattatga
    25801 cgagtcagtc ggtcaagata gcccatcaca aatgcagaaa gcacaaacgt cagatggata
    25861 atgacgtacc acatcagttt gttatccggg acatttttcg catccataaa gacgcgcagt
    25921 aagtgaatgg aagaaattgc cacaatcgac gctgctactt tgtttttcag cgacgttgcg
    25981 tccattttcc ccagccactt cagcttctct ttgttctcgg agatatccag ctgtgagacg
    26041 aaattctcat aaccggaaaa catcaccatc accagcaaac cgccaaccag cgtcatatcc
    26101 accagcgaca gcaacacgag gatcaaatct gattccgcca tcgagaagat attcggcagt
    26161 acgtgaataa tctcctggaa gaacttcagc gccagggcaa ctaacgccag cgaaaggcca
    26221 aagtacacgg gggcaagcag ccagcgagaa gcatacattg cattttcaag aaaacgttcc
    26281 atacattcct gtcgtgtagt taaaacgggc aacagtatat cgcaattgag taaacagcac
    26341 gcaacagcaa atcaacatat acgttaaacc tttgtcgcat aattctgagg tttagggatc
    26401 ttcctggttc ccttatcaac gcatcgagag cgtctaccct taaaggacgt tgaacctgaa
    26461 gggagaaaaa cgatgtctca tttaaaagac ccgaccacgc agtattacac tggtgaatat
    26521 cccaaacaga aacaaccgac gccaggcatc caggcgaaga tgacaccgat accggattgc
    26581 ggcgagaaaa cctatgttgg tagcggtcgc ctgaaagatc gtaaagcact ggtgacaggg
    26641 ggcgattccg gaataggtcg cgctgccgcc atcgcttacg cgcgtgaagg ggctgacgtg
    26701 gcgatcagtt atcttcccgt ggaagaagaa gacgctcagg atgtgaaaaa gatcattgaa
    26761 gaatgcggac gcaaagccgt tctgctgcca ggcgatttaa gcgatgagaa attcgcccgt
    26821 tcgctggttc acgaagcgca caaggcgtta ggcgggctgg atattatggc gctggtcgcc
    26881 gggaaacagg ttgccattcc ggatattgca gacctcacca gcgaacagtt tcaaaagacc
    26941 tttgccatta acgttttcgc gctgttctgg ctaacccagg aagcgatccc cctgctaccg
    27001 aaaggtgcaa gtgtcatcac cacttcgtca atccaggcat accagccaag tccgcattta
    27061 ctggactatg cggctacgaa ggcggcgatt ctgaactaca gccgtggcct ggcaaaacag
    27121 gtcgcggaga aaggtattcg ggtgaatatt gtcgcgccag gccctatctg gacggcactg
    27181 caaatttccg gcggacaaac gcaggataag atcccgcagt ttggtcagaa aacgccgatg
    27241 aagcgtgcgg ggcaaccggc ggaactggcc cctgtatatg tttatctggc aagtcaggag
    27301 tcgagctacg tcaccgcaga agtgcacggc gtgtgcggcg gcgagcattt aggttaaaaa
    27361 aatgcccggt tgtgaaaagc aaccgggcat cattgtgaat tcactcttac ctgatgtggc
    27421 gaaaacgcct tatccgccta caaacgcatg aagattcaac atgttgcaat agattttgta
    27481 ggcctgataa gcgtagcgca tcaggcattg agcacctaat gcctgatgcg tcgtgaacgc
    27541 cttatccggc ctacaaacgc acgaagattc aacatgttgc aatagatttt gtaggcctga
    27601 tgagcgtagc gcatcaggca attctacttt acttcgcttt ggcggtttct tcgccgacca
    27661 gacctatctt caggtaaccc gcctgatgca gcgtatccat taccttcatc aacgtctcgt
    27721 aatcgacggt tttgtccgct cggaagaaga tggtggtgtc tttcttgcct tcggttaacg
    27781 cattcaacgc cgtaatcatt gtttcatcgg tgaccggatc gttaccgata aacatcgagt
    27841 tgtctgcctt caccgacaga taaaccggtt tttccgggcg cggctgcggc gtgctggtag
    27901 aagcaggcaa gttcaccttc acatctaccg tcgctaacgg tgccgccacc ataaagataa
    27961 tcagcagaac caacatcacg tcgataaacg gcgtcacgtt gatatcatgc atttcgccgt
    28021 tatcgtcgag gttttcgtta agatgcattg ccatcggaca ttatcctgcg cgtaattttt
    28081 gtgcgacacg aaccggatgc gcagcggcgc tggcttccag atccaggtca cggctttgca
    28141 gcaacaatac ctgcgctgca acatcaccca gcatcgcttt aaagccgcca atctggcgtg
    28201 caaatacgtt atagataacg accgcaggaa tcgctgccac gaggccgatt gccgttgcta
    28261 acagagcttc tgcgataccc ggcgcaacga ctgccaggtt agtggtctgc gtttgcgcga
    28321 taccaataaa gctgttcatg atgccccaga ccgtaccaaa cagaccaacg aacggagaaa
    28381 tcgcgccgat ggttgccaga tagccgttac cgcggcccat ttgacgaccc actgcggcga
    28441 cccgacgctc cagacggaag ctggtacgtt ctttaatacc ttcgttatcg tcgctgcctt
    28501 ctgacagttc cagctcattc tgcgcttcat tgagcaaatg caggcttaag cttttgctac
    28561 caaaatcagc ggcgatatcg ttggcctggt ttaaggaacg tgcttcagcc agcagttgct
    28621 gctcgcgctt aaggcgacgc ttctgattga agaactctac gctcttactg aagaagattg
    28681 cccaggtgac tacagaggcc aaaataagcc caatcatcac gcacttaacg acaatatcgg
    28741 cgtactgata cataccccag acggaaaggt ccgtctgcat taaattatta cccactgtgt
    28801 atctccagga cgcaagtcac aaaatctgcg cataataata tcaaaacgac gtcgaattga
    28861 tagtcgttct cattactatt tgcatactgc cgtacctttg ctttcttttc cttgcgttta
    28921 cgcagtaaaa aagtcaccag cacgccattt gcgaaaattt tctgctttat gccaattctt
    28981 caggatgcgc ccgcgaatat tcatgctagt ttagacatcc agacgtataa aaacaggaat
    29041 cccgacatgg cggacaaaaa gcttgatact caactggtga atgcaggacg cagcaaaaaa
    29101 tacactcttg gcgcggtaaa tagcgtgatt cagcgcgctt cttcgctggt atttgagagt
    29161 atggaagcca aaaagcacgc gacgcgcaat cgcgccaatg gcgagttgtt ctatggacgg
    29221 cgcggaacgt taacccattt ttccttacaa caagcgatgt gtgaactgga aggtggcgca
    29281 ggctgcgcgc tatttccctg cggggcggca gcggttgcta attcgattct tgcttttgtc
    29341 gaacagggcg atcatgtgtt gatgaccaac accgcctatg aaccgagtca ggatttctgt
    29401 agcaaaatcc tcagcaaact gggcgtaacg acatcgtggt ttgatccgct gattggtgcc
    29461 gatatcgtta agcatctgca gccaaacact aaaatcgtgt ttctggaatc gccgggttcc
    29521 atcactatgg aagtccacga cgttccggcg attgttgccg ccgtgcgcag tgtggtgccg
    29581 gatgccatca ttatgatcga caacacctgg gcagccggtg tgctgtttaa ggcgctggat
    29641 tttggcatcg atgtttctat tcaggccgcc accaaatatc tggttgggca ttcagatgcg
    29701 atgattggca ctgccgtgtg caatgcccgt tgctgggagc agctacggga aaatgcctat
    29761 ctgatgggcc agatggtcga tgccgatacc gcctatataa ccagccgtgg cctgcgcaca
    29821 ttaggtgtgc gtttgcgtca acatcatgaa agcagtctga aagtggccga atggctggca
    29881 gaacatccgc aagttgcgcg agttaaccac cctgccctgc ctggcagtaa aggacacgaa
    29941 ttctggaaac gagactttac aggcagcagc gggctatttt cctttgtgct taagaaaaaa
    30001 ctcaatgatg aagagctggc gaactatctg gataacttca gtttattcag catggcctac
    30061 tcgtggggcg ggtatgaatc gttgatcctg gcaaatcaac cagaacatat cgccgccatt
    30121 cgcccacaag gcgagatcga ttttagcggg accttgattc gcctgcatat tggtctggaa
    30181 gatgtcgacg atctgattgc cgatctggac gccggttttg cacggattgt ataacattgc
    30241 cacttttgga caattttgca gacattttat tgtgaaaagt cttaaattgt tgcgtccggg
    30301 atcaaggcgt cccggacgat tcaggagtac aataggcaga taaaggctta aacgctgttc
    30361 cacaggaaag tccatggctg ttattcaaga tatcatcgct gcgctctggc aacacgactt
    30421 tgccgcgctg gcggatcctc atattgttag cgttgtttac tttgtcatgt ttgccacgct
    30481 gtttttagaa aacggcctgc tgcccgcctc atttttgcca ggcgacagct tgttgatact
    30541 ggcaggcgca ttgattgccc aaggggttat ggattttctg cctacgattg cgattctgac
    30601 cgccgcagca agtctgggct gctggctaag ttatattcag gggagctggt tagggaatac
    30661 caaaacggtg aaaggctggc tggcacagct tcctgctaaa tatcaccagc gcgccacctg
    30721 catgtttgac cgccacggtc tgctggcgct gctggctgga cgttttcttg catttgtccg
    30781 tacgctgctg ccaaccatgg cgggaatttc cggtctgcca aaccgccgct tccagttttt
    30841 caactggtta agtggattgc tgtgggtcag cgtggtaacc agttttggct atgccttaag
    30901 tatgattccg ttcgttaaac gccatgaaga tcaggtaatg acgttcctga tgatcctgcc
    30961 aattgccttg ttaaccgctg gcttgttagg cacgctgttt gtggtgatta aaaaaaaata
    31021 ctgtaacgcc tgacgatttt ccccgttccc ggctgctgta ccgggaacgt atttaattcc
    31081 cctgcatcgc ccgcattctt gccgcatctt cccccggcgt cacaccgaag taacgtttaa
    31141 actcacggct aaattgcgat gcgctttcat agccgacgcg catcgctgct gcgctggcct
    31201 tcatgccgtc atggatgatc atcatccgcg ccttatgcag acggtaattc ttcaaatact
    31261 gcaacggcga ggtgctggtg acagacttaa aattatggtg gaacgccgat acgctcatgt
    31321 tggcttctgc cgccagttgc tcgacgctca ggttttcggt gtatttattc tcaattcgtt
    31381 tcagcacgcg gctaatcaga ctgaagtgag tctggcgact gaccagcgcc agtaacgcgc
    31441 cgccgcaagg tccggtcagc acgtagtaca gaatttcgcg gatgatctgt ttgccgagaa
    31501 tacgcgcatc cagtggtcgc tccatcacgt cgagtaaccg ctccgccgcg cataaaatct
    31561 cttctgataa cgtggcggag ttaatcccgc tggctgccat cgacggctgg aaatgctcat
    31621 cttcgccaat gtccatcaac agttcctgta actgcaaaat atcgacattg agacgcaacc
    31681 ctgccagcgg cacctctgac gtggcatagg tttcgcactc aaacggcaac ggcaccgtca
    31741 gcagcaggta ttcattggca tcataacgaa acacgcgttc attgatataa ccgattttat
    31801 gcccggaaaa gagaattatg atgccaggct cgtacatcac cggtgtacgt gcgaaaggcg
    31861 tctcgccata caacaaacgc acatcgggca acagttctga caaactattt tctttatttt
    31921 tcagtttatt aactttatcc gccagcaagc gacaaatctc ttcacgtttc atatcgcgta
    31981 atttcttagg aataatgcgg caatttgatt gtgcgcaatt ttgtagcatt tctccagcac
    32041 tctggagaaa taggcaagac attggcagaa atgagcattg agagccaggg cgctggcgat
    32101 cacaatgaaa aacatcaggc agatcgttct ctgccctcat attggcccag caaagggagc
    32161 aagtaatgaa caactttaat ctgcacaccc caacccgcat tctgtttggt aaaggcgcaa
    32221 tcgctggttt acgcgaacaa attcctcacg atgctcgcgt attgattacc tacggtggcg
    32281 gcagcgtgaa aaaaaccggc gttctcgatc aagttctgga tgccctgaaa ggcatggacg
    32341 tgctggaatt tggcggtatt gagccaaacc cggcttatga aacgctgatg aacgccgtga
    32401 aactggttcg cgaacagaaa gtgactttcc tgctggcggt tggcggcggt tctgtactgg
    32461 acggcaccaa atttatcgcc gcagcggcta actatccgga aaatatcgat ccgtggcaca
    32521 ttctgcaaac gggcggtaaa gagattaaaa gcgccatccc gatgggctgt gtgctgacgc
    32581 tgccagcaac cggttcagaa tccaacgcag gcgcggtgat ctcccgtaaa accacaggcg
    32641 acaagcaggc gttccattct gcacatgttc agccggtatt tgccgtgctc gatccggttt
    32701 atacctacac cctgccgccg cgtcaggtgg ctaacggcgt agtggacgcc tttgtacaca
    32761 ccgtggaaca gtatgttacc aaaccggttg atgccaaaat tcaggaccgt ttcgcagaag
    32821 gcattttgct gacgctaatc gaagatggtc cgaaagccct gaaagagcca gaaaactacg
    32881 atgtgcgcgc caacgtcatg tgggcggcga ctcaggcgct gaacggtttg attggcactg
    32941 gcgtaccgca ggactgggca acgcatatgc tgggccacga actgactgcg atgcacggtc
    33001 tggatcacgc gcaaacactg gctatcgtcc tgcctgcact gtggaatgaa aaacgcgaga
    33061 ccaagcgcgc taagctgctg caatatgctg aacgcgtctg gaacatcact gaaggttccg
    33121 acgatgagcg tattgacgcc gcgattgccg caacccgcaa tttctttgag caattaggcg
    33181 tgccgaccca cctctccgac tacggtctgg acggcagctc catcccggct ttgctgaaaa
    33241 aactggaaga gcacggcatg acccaactgg gcgaaaatca tgacattacg ttggatgtca
    33301 gccgccgtat atacgaagcc gcccgctaag ctttttacgc ctcaaacttt cgttttcggg
    33361 catttcgtcc agacttaagt tcacaacacc tcaccggagc ctgctccggt gagttcatat
    33421 aaaggaggaa cgtatggcta atccaaccgt tattaagcta caggatggca atgtcatgcc
    33481 ccagctggga ctgggcgtct ggcaagcaag taatgaggaa gtaatcaccg ccattcaaaa
    33541 agcgttagaa gtgggttatc gctcgattga taccgccgcg gcctacaaga acgaagaagg
    33601 tgtcggcaaa gccctgaaaa atgcctcagt caacagagaa gaactgttca tcaccactaa
    33661 gctgtggaac gacgaccaca agcgcccccg cgaagccctg ctcgacagcc tgaaaaaact
    33721 ccagcttgat tatatcgacc tctacttaat gcactggccc gttcccgcta tcgaccatta
    33781 tgtcgaagca tggaaaggca tgatcgaatt gcaaaaagag ggattaatca aaagcatcgg
    33841 cgtgtgcaac ttccagatcc atcacctgca acgcctgatt gatgaaactg gcgtgacgcc
    33901 tgtgataaac cagatcgaac ttcatccgct gatgcaacaa cgccagctac acgcctggaa
    33961 cgcgacacac aaaatccaga ccgaatcctg gagcccatta gcgcaaggag ggaaaggcgt
    34021 tttcgatcag aaagtcattc gcgatctggc agataaatac ggcaaaaccc cggcgcagat
    34081 tgttatccgc tggcatctgg atagcggcct ggtggtgatc ccgaaatcgg tcacaccttc
    34141 acgtattgcc gaaaactttg atgtctggga tttccgtctc gacaaagacg aactcggcaa
    34201 aattgcaaaa ctcgatcagg gcaagcgtct cggtcccgat cctgaccagt tcggcggcta
    34261 acgtgcaaat tctcccggtg gcggtaatgt tccgctaccg gacttttcag aaatcattta
    34321 ttcccctcgc gtcccgcccg ttgttactct tccttgttca ggaatgccaa atataaggac
    34381 atcatcatgc agagccggaa gctcttaaaa gaacaactca tctatatccg ggataaacgc
    34441 aacggagagg tgaaaaacag atgaaaataa tacttctgtt tttagcagcc ctggcaagtt
    34501 ttaccgtaca cgcgcagcct ccttcacaga ccgtagaaca aacagtccgg cagatttatc
    34561 agaactataa atcagatgcc agtacgcctt atttcggtga aaccggagag cgggcgataa
    34621 cttcagcgcg tattcaacag gcgcttaccc tgaacgacaa tcttacacta ccgggcaata
    34681 ttggctggct ggattatgat ccggtttgtg attgtcagga ttttggcgat ctggtgttag
    34741 aaagcgtagc gataacccaa actgacgtcg atcatgccga tgccgttgtg cgctttcgta
    34801 tctttaaaga tgataaagaa aagaccacac agacactgaa aatggtggcg gaaaatggtc
    34861 gttgggtcat tgatgatatt gtcagcaatc atggcagcgt cttacaagca gttaatagcg
    34921 agaatgaaaa aacgctggcc gctttagctt cattgcagaa agaacagcca gaatcctttg
    34981 ttgctgaact ctttgaacat attgctgatt atagctggcc gtggacgtgg gtggtttccg
    35041 actcttaccg ccaggcggtt aatgccttct ataaaaccac cttcaagacg gccaataatc
    35101 ccgatgaaga tatgcaaata gaacggcaat ttatttacga caatccgatc tgttttggcg
    35161 aagagtcgct attttcacgc gttgatgaaa ttcgagtcct ggagaaaacc gccgattccg
    35221 cccgcattca tgttcgtttt acgctgacca atggcaacaa tgaagagcaa gagctggttt
    35281 tacagcggcg cgaaggtaag tgggaaatcg ctgattttat ccgcccgaac agcggcagcc
    35341 tacttaagca gattgaggca aaaaccgccg ccagattaaa gcaatgagct gaattaaata
    35401 acaattagcc ggaacaataa ataaaaggga acactatatg aaaacgattt tcaccgtggg
    35461 agctgttgtt ctggcaacct gcttgctcag tggctgcgtc aatgagcaaa aggtcaatca
    35521 actggcgagc aatgtacaaa cattaaatgc caaaatcgcc cggcttgagc aggatatgaa
    35581 agcactacgc ccacaaatct atgctgccaa atccgaagct aacagagcca atacgcgtct
    35641 tgatgcacag gactattttg attgcctgcg ctgcttgcgt atgtacgcag aatgataaaa
    35701 aaatccccgg cagcatgtca gttgccgggg atttttttta acgtccaacc gccgctttag
    35761 ggcgtttctt cgcaccagca ttcaccggac gagattgcgt agacgacgct ttttttgccg
    35821 tagcaggcgt ctgacgctga gtcgccatcg gcgtatgttt agtcaacgcc ggacgggtat
    35881 tgcggttctg gcgacgagct tcacgcatct cttcaatggt tggcgcaggc actaagcaat
    35941 cgcgacggct gccaatcaga tgctttttgc ccatcgcttc cagcgcctga cggattaacg
    36001 gccagtttgc cggatcgtgg taacgcaaca acgctttatg caaacgacgc tgtttgtcgc
    36061 ccttcggtac gaagacgcct tcgctcttat aaccaatctt cgccagcggt tttttcccgg
    36121 tgtaatacat ggtggttgag ttcgccagcg gcgatgggta gaagttctgt acctggtcga
    36181 gacggaaacg atgctttttc agccacagcg ccaaattcac catatcttca tcacgcgtac
    36241 cggggtgcgc agagatgaaa tacgggatca gatactgctc tttacctgcc tgtttcgagt
    36301 aggtatcgaa cagctcttta aagcggtcat agctgcccat gcccggcttc atcatcttcg
    36361 acaacggccc ttcttcggta tgttccggag caatcttcag ataaccgccg acgtgatggg
    36421 tcgccaactc tttgatatag cgcggatctt ccacggcgat gtcataacgt acgcctgagg
    36481 cgataaggat ttttttaatg cctttcagat cacgcgcacg gcgatagagg ttgatcgtcg
    36541 gttcgtggtt agtgtccatg tgcggacaaa tatccggata aacgcacgac aaacggcgac
    36601 aagtttgttc agcgcgtggc gatttgcagc gcaacatata catgttggca gttggcccac
    36661 cgagatcgga aatcacgccc gtaaaacctg gaacggtgtc gcggatcgct tcgatctcat
    36721 taatgatcga atcttccgaa cggctctgaa taatgcgccc ttcgtgctcg gtaatagaac
    36781 agaaagaaca gccgccaaag cagccgcgca taatgttgac cgaaaaacgg atcatttcgt
    36841 aagccggaat acgggcattg ccataggccg gatgtggcac gcgcttgtac ggcagcgcaa
    36901 aaacgctgtc catctcttcg gtagaaagcg ggatagcagg cgggttgatc cacacatagc
    36961 ggtcaccgtg tttttgcatc aatgcgcggg cgcagcctgg gttggtttcg tggtgcagaa
    37021 tgcgcgaagc atgggcgtac agcactttat cgcccttcac tttctcgaaa gaaggcagca
    37081 atacgtaggt tttttcccac ggtttctggc gcggtggctg cacgattaca gctttcgctt
    37141 cctgcttttt cggcgcaaca ggtttgttat ccgcacacgg caaatcttcg ccatacggat
    37201 gcgggattgg ggcgattttc cctggggtat caagacgggt ggaatccaca ccgctccagc
    37261 caggcagcgc ctcttttacg ataattgcgg tattacgcac atcgcggatt tcactaatcg
    37321 gctcgcccat cgccagacga tgcactactt ccaccagcgg acgttcaccg ttaccaaaca
    37381 tcagcatgtc ggctttcgaa tctaccagaa cggaacggcg cacggtatcg gaccagtaat
    37441 cataatgcgc ggtacggcgc aggctagcct caataccgcc gaggatcacc ggcacatctt
    37501 tccacgcctc tttacaacgc tgggtataaa ccagcgtggc gcgatccggt cgcttacccg
    37561 cgacattatc tggcgtgtag gcatcatcat gacgtaaacg gcgatcggcg gtatagcggt
    37621 tgatcatcga atccatgttg ccagcagtaa caccaaaaaa cagattcggt ttacccagac
    37681 gcataaagtc gtctttgctg ctccagtccg gctgggcgat gatcccgacg cgaaagccct
    37741 gcgcttccag catacgaccg caaatcgcca tcccgaagct tgggtgatcg acatacgcgt
    37801 cgccagtaac caaaatgatg tcgcagctat cccagccaag ttgatccatc tcttcacgag
    37861 acatcggcaa aaacggtgcc ggtccaaaac aggcggccca gtactgcggc caggagaaca
    37921 ggtcgcgatc cggttggatc agggagatag agctcatttt gcttccagaa atgataaaaa
    37981 aataatcaaa ggccggggat tataagccgg aacgaaagag aaatcgaaag gtattccata
    38041 ctcgccctcc tcgggcgagt atgaagatta cggtaccgga ttgaccaaca gttgcccaat
    38101 cgagccacgg tccgccattt ccagcgtctg actgttgaag tagaacggga agtgcgccca
    38161 ggaaggctga ccgaaataaa caagcagctc cacttgtccg tcaacccaaa cggtatcttt
    38221 ccagcctctg tcttccggga acggcatcgc gccattcacg ttacggatct ggaacattac
    38281 gccttcaata tggaacgctt gcggctcgtc cgcgcgtacc gtccagcgtt cccacgttcc
    38341 ttgctgcgcg gtgacatcaa tacggttgac gtcccacagc tgtccattaa tacccgggtc
    38401 atcacccaga ctgatatcgc gactgcgaat tggcgaacca gccatgattt cagttggcag
    38461 caagcgcatc ggaagactgt ctgtgaccag cggcagaagg ccggttgggc gcagcgttag
    38521 caccaaggta gaaaccagaa tactggatgg ctcaaagaag ccgcgaatac gatcaacaat
    38581 gctcgccgct tcaccacagg tgatcgacac ttcatcgccg ttgctcatat ccaccagaat
    38641 ctcgcggcgc tcgcccggtg ccagcgaaag ttgcttcacc gatacaggag caggcaggaa
    38701 tccctgatcg ccagaaatca catgtaacgg gcgaccatcg ctcatctgta gttgatagcg
    38761 acgagagttc gacgcgttca gcagtcgcaa gcgcacccag ccacgcgaga cttcaacgta
    38821 cgggctttgt acaccgttaa ccagcagcgt atcaccaaca aagccgccgc ttcccggttc
    38881 gttgtattct ggcgtaccaa agttatccag ccgtttatcc tggataatga ccggaaaatc
    38941 atccacacca taatggttgg ggataggcag cgacttgctg acttcatctt ccaccagcca
    39001 cattccggca aggccgttat agacctgctg agccgtgcgg ttgggagtat tggcgtgata
    39061 ccacagagta gctgcgttct ggcgaatagg cagtacgggt gcccagtcag cgtttggcga
    39121 catcatccgt gccggaccgc ccatcagcgg gcctggtacc tgtagcccgg ccaccgtcat
    39181 tgagacattt tctgtcaggc ggttgctgta aataagctta acatcgtcgc ccttccagac
    39241 gcggatagtc ggccccaggt aacgaccatt gattccccag accgacgcgc gtgtccctgg
    39301 cgtaaatgac cagtgcgcac gttgtacagt cataaacagc ggttgcccac ggcgagattc
    39361 aagtagcggc ggaacgggta gcggttgctg ttgcccggct gcgctggcct tcaggggaac
    39421 agcgcctgca caaagtgcaa tccccgatgc ctgaatgaac tgacgccgac tgagtgacat
    39481 atttgctcca tgtaaaactg gctaaagtaa taccgtgcag gaaagtgttc cccgcaaaaa
    39541 tacagattgc cgacttaaac ttttccggcg gcttcgcgtt ctgcgacttc tttatcgagc
    39601 tcggcgattt tttgttccat tatcgaacga caatgggcag ccagctcacg aacctgatct
    39661 ttgccatact gactgacgtc aattggcggc agcatttcga caatcaccag accgttgtgc
    39721 agtcgattaa gattaatctt attcgaagtt gtagagacgc acacgggaat aatcgggacg
    39781 cccgccgcaa ttgccgcgtg aaatgctcca gtcttgaacg gtagcaggcc gcgaccacgg
    39841 ctgcgggttc cttccgggaa catccagatg gaaatacggc gttttttgaa atgattcacg
    39901 acttccgcaa tggtgccgtg agctttagtg cgattgtttc tgtcgatcaa taagttgccg
    39961 gttaaccagt acaactgccc gaagaagggg atccacagca agctcttttt acctaccgtc
    40021 accgtcggcg gttgcacaat gttcgatgct gtcaccatgt catagttgtt ctggtggtta
    40081 gcgatataga tagcattgcc gtagttttca gcatctgcag gtttgcggca ctcaactttc
    40141 aggccgaaca gtggcgcaag acggccaaac atatgcccaa aggtggccac atgtttcggg
    40201 ttacgcgggc tgaaaaggca gtaaatggag ccgaatacac agactaagat gctgtaaatc
    40261 acggtaataa taagacgaaa gatatatagc ataacaccct ctgaaggtct gagagactgg
    40321 cattgtacgt cacctgaaat caggttaggg cattgttaaa agccgctctc tggaaaagcg
    40381 tattgttaat cgcaacgcat gaataaacaa cggtttcaag ggaattttta tcgtttccgg
    40441 gggttgaata cgctgccgga tgacgactta acgtttcatc cggcgttcct tgctagcggg
    40501 aggaaacagc gccctccccg gcatattact cttcgctatc accgctgctg gcacggcgag
    40561 gagagtcgat ctcaacacga tcgatacgct gcaaaccgcg catcaacgta ccgcggcgtc
    40621 cacgttcgcc agtgactttc tgtagctctt ccggacgcag tttaattttg cgtttcccaa
    40681 catgaatggt cagcgtgctt tgcggcggca gaacgtacag ttgcgccaga ccatcctcgc
    40741 cacgcgcggc ttctgccgat ggaatgttga taatcttgtt gcctttgccc ttcgacagct
    40801 gcggcagatc acttaccggg aacatcaaca tacggcctgc ctgagtgatt gccagcagca
    40861 tatcggaagc atcttcaatc accaccggcg gcataacatg ggcattttcc ggtaaggtga
    40921 tcaaagcctt acccgcacgg ttacgcgcta ccagatcgtt aaaggtgcag acgaaaccgt
    40981 aacccgcatc ggaagccatc agcagtttct gatcgtcgct ttccatcagc atatggtcaa
    41041 cggtcgcccc aggcggcaac gttaatttgc cggtgagtgg ttcgccctga ccacgcgccg
    41101 acggcagcgt aatcgggtcg atggcatagc tacgaccggt ggaatcaaca aacactaccg
    41161 gttggttact cttacctttc accgccgctt tgaagctatc acccgcttta taattcaggc
    41221 ccggcgcgtc gatatcatgg cctttagcgc tgcgtaccca gcccatctgc gacagcacaa
    41281 tggtgacagg ttcagacggc agcatgtcgt gctcgctcat cgctttcgct tcttcgcgtt
    41341 cctgcaacgg cgaacgacga tcgtcaccgt aggcttgcgc gtctgcctgc agttctttct
    41401 tcagcaggtt attcatttta cgctcggaag ccaaaatgcc ctgcaactgg tcgcgctctt
    41461 tttccagctc actctgctca ccgcgaatct tcatctcttc cagtttggca agatgacgca
    41521 gtttcagttc gaggatcgct tccgcctggg tttccgtaag gccaaaccgc gacatcagcg
    41581 ccggtttcgg ttcatcttcg ttacgaatga tctcaatcac ttcgtcgatg ttgagaaacg
    41641 ccaccagcaa accttccagg atatgcaggc gcttgaggac tttctccaga cgatagttca
    41701 gtcggcggcg cacggtatcg cgacggaaca ccagccattc ggagaggatt tccagcaggt
    41761 ttttcaccgc cggacgacca tccagaccga tcatattgag gttaatgcga tagctctttt
    41821 ccagatcggt ggtagcgaag aggtggttca tcacctgatc catatccacg cggttggaac
    41881 gcggcacaat caccagacgg gtcgggttct cgtggtcaga ttcatcgcgc agatcgtcaa
    41941 ccatcggcag ctttttgttg cgcatttgcg cagcaatttg ctccagtacg cgcgcacctg
    42001 aaacctgatg cggtaatgcg ctgataacca ccgcgccatc ttctttcttc cacaccgcgc
    42061 gcatacgcac tgaaccacgt ccgttctcgt agattttacg aatctcggcg cgcgaagtga
    42121 taatttccgc ttcagtcgga taatccggcc cctgcacgat atccagcagc tgatcgagcg
    42181 tagttttcgg ctggtcgatt aatgcgattg ccgcctgagc tacttcacgc aggttatgcg
    42241 gtggaatatc ggtcgccatg ccgacggcaa taccggtggt gccgttaagc aaaatgtttg
    42301 gcagacgggc aggtagcatt ttcggctcct gcaaagtgcc gtcgaagttt ggcacccagt
    42361 cagccgtccc ctgccccagc tcgctcaata gcagctcgga atatttcgac aaccgggatt
    42421 cggtgtaacg cattgccgcg aacgatttcg gatcgtccgg cgcgccccag ttcccctgac
    42481 catcaaccag cggataacgg taagagaacg gctgcgccat caggaccatc gcttcataac
    42541 aggcgctatc gccgtgcgga tggtatttac ccagtacgtc accgacggta cgggccgatt
    42601 ttttaaattt ggcgctggca ttcaggccca gttcagacat cgcatacaca atgcggcgct
    42661 gaacaggttt cagaccatca ccaataaacg gcaacgcacg gtccatgatc acgtacatgg
    42721 agtagtttaa gtaggcgttt tccgtaaatt catgtagcgc aaggcgctct gccatatcgc
    42781 tcattaattc tgattcctca acttattcgc cagccttcaa aggggcaata ttgccggaga
    42841 tactacatta tctgccgcgt cgagtcacag gagatcagcg tgaatagcgc cgccggacgt
    42901 gctccgacgg cgataaaggc atcaatgtgc cttgatatac aactctttgc tgtagacttg
    42961 atcggcatag agggcagcac aaggttgcgc ccatgccaca ccactcggca tgggggtccg
    43021 ctccgggcga ttcgagaagt tgaggttatg cgagtccagg gaagcctaaa gcatcaaagt
    43081 agtcgactgt aagtatacgg ttcagcccaa tttagctgtg tctccaccag tgccccgcgc
    43141 ctcctgccat aagtgcggct tgctcatgga tatccccgag acggcttacg gcgcaccgga
    43201 taataaattt cgcgtataca tggtattcct taccggagaa attataagcc caacaacaat
    43261 acgcctgtga cattgttcgc tcaaacttat cgcgagtgat ttggctcaca ctccggtaaa
    43321 gattccccat cacgccaacg gcacataaac atccgtttcc agtcgctgct ctgtcacccc
    43381 ttcggcaaga ttggtgtagt gaaacagaat cggtgcttta cgcattttct cgccgcttgc
    43441 aggcagccag tggcgaataa tgccccatat cgtgtgtgaa atatcgtcca gctcgccaac
    43501 gtggcgggcc acggcataac gtccaccggt aagttcacca ttgctcacac cataacgatt
    43561 atcgggaatc ggttcgctaa cgctgccgca gatatcaaag cgaaacgctt ccggtgcggt
    43621 ggtggcaggg gcgtcccagg cgacgccaaa agtctggctt tggttaacag gagaaagccc
    43681 cgtctcttta cgccacatga taaatttcgc cacactgtag ttgagcaact cagggctaca
    43741 gcggtgcggc aacatcgcca cagggattga tgggaaatcg ataatgttta catccagtgt
    43801 caggtttgtc attttgcctc cctgcgaaat tattcccggg aacgcgtccc gggagcggta
    43861 acaattacgg atttactttg cggatctgtt tgacgtcaat ttcaacagaa ttccagtctt
    43921 tatcgacttc accctgaatc tcaaccgtat ctttcggcgt caccgtcaca ccgttccagc
    43981 gtttgtgatc gatatcaaca ttgatagtac cgctggcatc tttaaacacg tagagatcgt
    44041 cagagatgcg ttcaacgata ttgccgcgca gggttaccca ggtgtcgtca cgcagggatt
    44101 ttgcgctttc tacagtcgtt acgctgccgt tcggcccctg aaatcctccg gcctgacttt
    44161 gcgttaccga tgggccagaa aaaccgccct gctctgctgc catcaccggt gcactgcaca
    44221 gggccattac tgcgattact gctgcgaatt ttttcatgtt tattactccc tttaatgtct
    44281 gtttccgagc atttaacaag atagtcctta acaacttctt aagggaaaaa aataaaattt
    44341 aatgctgtac agagcgcgtt acaacacggt ttactggcag caaatacggt tatcgcaggg
    44401 atgaaaaaat gcgaatttta ctgatagaag atgacatgct gattggtgac ggcatcaaaa
    44461 ctggccttag taaaatgggt tttagtgtcg actggtttac acaaggtcgt cagggaaaag
    44521 aggcgcttta tagcgcacct tatgatgcgg tgatcctgga tttaacctta ccaggcatgg
    44581 atggtcgcga tattttgcgc gaatggcgag agaaaggtca gcgtgagccg gtactgaacc
    44641 tgaccgcgcg tgatgcgtta gcggaacgtg tagaagggct gcgtctggga gctgacgatt
    44701 atctgtgtaa accttttgcg ttgatagaag tcgccgccaa gctggaggct ctgatgcgcc
    44761 gaaccaacgg ccaggccagc aacgagctgc gccacggtaa cgtcatgctc gaccccggca
    44821 aacgtatcgc cacgctggct ggcgaaccct taacactgaa accaaaagaa tttgccctgc
    44881 tggaattact gatgcgtaac gctggtcggg tactgccgcg caaactgatt gaagagaaac
    44941 tgtatacctg ggacgaagag gtcaccagta atgccgttga agtgcatgtg catcatctgc
    45001 gacgcaaact cggtagtgat tttattcgta ccgtgcatgg tattggctac acattaggtg
    45061 agaaatgaaa tttacccaac gtcttagtct gcgcgtcagg ctgacgctaa tctttttaat
    45121 tctggcctcg gtgacctggc tgctttccag ctttgtcgcc tggaaacaaa caacggataa
    45181 cgtcgatgaa ttgttcgaca cccaactgat gctgtttgcc aaacggttaa gtacgctcga
    45241 tctcaacgaa atcaacgcgg cggatcgcat ggcacagacg ccaaataaat taaaacacgg
    45301 tcatgttgat gacgatgcgc tgacctttgc catctttacc cacgacggca gaatggtcct
    45361 taatgatggc gataacggag aagatattcc ctatagctat caacgggaag gttttgctga
    45421 cgggcaactg gtcggtgaag acgatccttg gcgttttgtc tggatgacct cacctgatgg
    45481 caaatatcgc atcgttgttg gccaggaatg ggaataccgt gaagacatgg cgctggcgat
    45541 tgttgccggg caattgatcc cgtggctggt cgcactgccg attatgttaa tcatcatgat
    45601 ggtactactg ggtcgtgaac tcgcgccgct gaacaaactg gcgctggcac tacgtatgcg
    45661 tgaccctgac tcggaaaaac cactaaacgc gactggcgta cccagcgaag tgcggccact
    45721 ggttgagtcg ctaaatcaac tgttcgcccg cacacatgcg atgatggttc gagaacgacg
    45781 ctttacctcc gatgcagctc acgaactacg tagcccgtta acagcgctga aagtgcaaac
    45841 cgaagttgcg cagctctctg acgatgatcc gcaggcgcgg aaaaaagcac tgctccaatt
    45901 acattccggg atcgatcgcg ctactcgtct ggttgatcaa ctgctcacgc tatcgcggct
    45961 ggactcactg gataaccttc aggacgtcgc ggagatcccg cttgaagatc tcctacaatc
    46021 gtcggtgatg gatatttacc acacggcgca gcaggcgaaa attgacgtgc gactgacact
    46081 caatgcccac ggcatcaaac gcaccggaca accgctattg ctaagtttgt tggtgcgaaa
    46141 tttgctggat aacggccccg gtgtgacacc agaggcactg gcgcgaattg gcgaacgctt
    46201 ctatcgccca cccggacaaa ccgctaccgg cagtggactt gggctatcga ttgtccagcg
    46261 aatcgccaag ctgcatgaca tgaatgttga gtttgggaat gcggaacaag gtggatttga
    46321 ggcgaaggta agctggtaac ttctctgtct atgagagccg ttaaaacgac tctcatagat
    46381 tttactaata gcaaaatata aaccgtcccc aaaaaagcca ccaaccacaa accagacaaa
    46441 taacgagcga gaatacatat agccaaaact cgaacatttc ccgcataaag agttttctta
    46501 agataagaat aataagtggc gtaagaagaa aaaatgctgc aatcattgct tgcaatatac
    46561 gatcttcgcc tgaaacggaa ggtatactgc aaatattgcc tcccgactca agcatccagt
    46621 catacttatt gaaagcaaaa aaagaatcta ttaaccaata agtaagcatt atatatagca
    46681 atgcttcaaa tattgtttta attttctgct ttaacatata tcagtacgct cataaaaatc
    46741 catttataat tatcaacatg acacgttgat attttcatct tttacttatt caaagccata
    46801 ttttcaatcc ccaaaaaaac cgaaccaaac gtgtaaatta ttccggtgcg agtaagaatt
    46861 atgaggaatg gctatcagta ttgtcatttt cagaaaatat ttatcctgca tcggtgagtc
    46921 agagctagat cagacttttg ctaaattcgc aaaagacttt gcacattttg ctaatttcac
    46981 agtaccgctc tgtgacgtac tatagtcgac aaacgtctca ccttgagatt aaaaaatgag
    47041 caacatcctg attatcaacg gcgcgaaaaa attcgcccac tccaatggtc aactgaacga
    47101 caccctgacc gaagtcgcgg atggcacact gcgcgacctc gggcatgatg tccgcatcgt
    47161 tcgcgccgac agcgactaca atgtcaaagc ggaagtacaa aactttctct gggctgatgt
    47221 ggtgatctgg cagatgccag gctggtggat gggcgcgccg tggacagtga aaaaatatat
    47281 tgatgatgta ttcaccgaag gtcacgggac tctgtatgcc agcgatggtc gtacccgcaa
    47341 agatccgtcg aaaaaatacg gttccggcag cctggtacag ggcaaaaaat atatgctttc
    47401 tctaacctgg aacgcaccaa tggaagcctt caccgaaaaa gatcagttct tccacggcgt
    47461 tggcgttgac ggtgtgtatc tgccgttcca taaagcaaac caattcctcg gtatggaacc
    47521 gctgccgaca tttatcgcta atgacgtgat aaaaatgcct gatgttcccc gctatactga
    47581 agaatatcgc aagcatcttg tggaaatttt tggttaacta gagctcaggc tttagaagga
    47641 gttaaccatg cttaccgtaa tcgcagaaat ccgtactcgt cctggtcaac atcaccgtca
    47701 ggcggtattg gatcagtttg ctaaaatcgt tccaaccgta ctgaaagaag aaggttgcca
    47761 cggctatgcg ccaatggtgg attgcgctgc tggcgtgagt ttccagtcta tggcaccgga
    47821 ttctatcgtg atgattgagc agtgggaaag catcgcgcac cttgaagcgc atctgcaaac
    47881 cccgcacatg aaggcgtaca gcgaagccgt aaaaggcgac gtgctggaga tgaatatccg
    47941 tattctgcag ccagggattt aatcctgcct tgtttgcccg gccatcctga ccgggcaatg
    48001 ttctttcctt taaacctcaa tctccgccat gtcgcctttc tcttgcaacc agttgcggcg
    48061 atcttccgag cgtttcttcg ccagcagcat atccatcatc gcgtcagtac gctgatcgtc
    48121 ttcatcatcg atagtcaact gcaccagacg gcgagtgttc ggatcaagcg tggtttcacg
    48181 caattgcatc gggttcattt cccccagacc tttaaaacgc tggacgttcg gcttgccttt
    48241 cttgcgtttt aattgctcaa gtacgccctc tttctcttct tccgtcagcg cgtaataaac
    48301 ctctttcccg agatcaatac ggtagagcgg tggcagtgcg acgtaaacgt gaccgtgttt
    48361 caccaacgcg cggaagtgtt ttacgaacaa agcgcagagc agcgtggcaa tgtgcagacc
    48421 atcagagtcc gcatccgcga ggatacagat tttgccataa cgaagctggc tcagatcgtc
    48481 gctgtcagga tcgataccga tcgctaccga aatatcatgc acttcctgcg aggccagcac
    48541 ttcgtcggaa gaaacttccc aggtgttaag gatcttacct ttcagcggca tgatcgcctg
    48601 atattcacga tcgcgcgcct gcttggcaga tccgcctgcg gagtcacctt ccactaggaa
    48661 cagttcggta cggttaaggc cctgcgcggt acaatcagcc agtttgccag gcagcgccgg
    48721 gccgctggtc agctttttac gcaccacttt tttagccgca cgcatacgac gctgggcgct
    48781 ggaaatcgcc atctctgcca gcaactccgc cgcctgaacg ttctggttca gccacaggat
    48841 gaaggcatct ttcaccacgc ccgaaacgaa tgccgcacac tgacgcgaag agagacgctc
    48901 tttggtctgc ccggcaaact gcggatcctg cattttaacc gacagaacat aggcgcagcg
    48961 atcccagata tcttccgccg acagcttcac gccgcgcggc agaatgttgc ggtattcaca
    49021 gaactcacgc atcgcgtcca acaggccctg acgcagacca ttaacatggg taccgccctg
    49081 catcgttggg ataaggttga cgtagctttc ggtcagcagt tcaccgcctt ccggcagcca
    49141 cagtagcgcc cagtccacag cttcagtatc gccagcgaaa ttaccgataa acggtttttc
    49201 tggcagcgtc ggtaaaccgt ttaccgcttc cgccaggtaa tcattcagac cgtcctgata
    49261 gcaccagcgc tgttcggtgt tgttgatctc atctttaaag gtaatctcaa cgccggggca
    49321 cagtactgct ttggccttca gcacatgcgt caggcgtgaa acagaaaaac gcgggctgtc
    49381 aaagaaggtt tcatccggcc agaagtgcac gctggtaccg gtattgcgtt taccgcaagt
    49441 gccgacaacc tgtaaatcct gcaccttttc gccattttca aaggcgatgt tataaacctg
    49501 accatcgcgg cgcacgttaa cttctacgcg cttcgacaag gcgttaacta ccgaaatccc
    49561 cacgccatgc aggccgccag agaactggta atttttgtta gagaatttac cgcctgcatg
    49621 cagacggcaa agaatcagtt caaccgccgg tactccttct tccgggtgaa tatccaccgg
    49681 catcccgcgc ccatcgtcaa tcacttctaa cgactggtca gcatgtaaga ttacatccac
    49741 gcgttttgcg tgacccgcca gtgcttcatc gacactgtta tcaataactt cttgccccaa
    49801 atggttaggg cgagtggtat cggtatacat ccccggacgg cggcgaaccg gctcaagccc
    49861 ggtgagtacc tcaatggcat cagcgttata agtttgcgtc atggtttaag ttagcaattc
    49921 gagttgatcg tcagagatgg tgcagaccaa gaaaatcgac gatcgggttg aaataatctt
    49981 cgaagcccgt gaatgcgtgg ttgccgcctt ctatgacagt ctggcggcag gaagcgtagt
    50041 acgccaccgc ctggcggtaa tccagcactt catctcccgt ctgttgcagc agccagatca
    50101 aatccggcgc ttccagcggg tcaatctgca tgactttgag atcgtaaata tggcgtgact
    50161 ctagcacata ttgctgcccg gtgtaggggt tctcgttctg accgagatag tccgtcagca
    50221 attcaaacgg gcgtaccgcc gggtttacca ccactgcggg cagcataaaa cattgcgaca
    50281 accaggtggc gtaatatccc cccagtgacg aaccgacaat acccagcgaa tcgccgccat
    50341 gttccaggac aatggattcc agcagttctg ccgcgtcgga aggatacggc ggcaactgcg
    50401 gaatgatcat ctcaacgtca gggtgatgtt ccgccagcca gttttttaac aagctcgctt
    50461 ttgcagagcg cggcgagctg ttgaaaccgt gtaaataaag aagcgtagac atcagtagcc
    50521 ttctgaagcg gtatcaggtt ggaaacgtgt gtccgccagg cgatgcacct cggtggtcag
    50581 cgtgccatca gcatgtaact cgagagtacg ccagccgggc gcgatagtat ccagcgtaaa
    50641 gttggaacag tgcggcttaa actgcacaca ggtcgacggc gttgccagca ggcggcgacc
    50701 gttccagtcg agatccagct cctgatgaat atgaccgcac agcaagtatt tgacgtgcgg
    50761 aaacttcgcc agcacggtat ccagttcgcc cgcgttacgc agactgtgtt gatcgagcca
    50821 actacaaccc gcaggtagcg gatgatgatg cagcagcagc aacgtatggc gttctggcgc
    50881 atcggccagt ttacgttcca gccactcaag ctgaaactcg ctcagctcac cgtgcggcac
    50941 gccaaacacc tggctatcca gcaacaggat ttgccattgc tcaccaataa acacccgctt
    51001 cgccggggag atacccgcat cctgtaacgc gctgtacatc gcgggctgga aatcgtggtt
    51061 gcccggcagc cagacgcagg gcgcacgaaa acttgcgatg ccttcagcga aatgctgata
    51121 ggccgcagag gattgatcct gcgctaaatc acctgtcgcg acaatcaggt cgaattcgtg
    51181 ctggtgtgga cgaatcgcct ccagcaccgc ctggtagctt tcccaggtgt ttacccctaa
    51241 cagggcttcg tgcttttgtg caaacaggtg agtatcggta atttgtaaaa tccttcctct
    51301 ggcctcacca gccagaggaa gggttaacag gctttccaaa tggtgtcctt aggtttcacg
    51361 acgctagtaa accggaatcg ccatcgctcc atgtgctaaa cagtatcgca accagtccgc
    51421 taaaaactgg ttaatttgat gcttttcgtc gcgttgatgc aactttttat taggataatc
    51481 ataccgcgct ttgaagcgaa aaatctgctg gcttgaacac acttcagcca ccatcgcgtc
    51541 atgatacaga cgcaccgtca ttgacggaag gctccagtaa ctgatcgcgg gcgcagtctg
    51601 ttctattgtc actagggtag tgtatcgggt cgattccaca atcgtcagcc gatattgtgc
    51661 gtttgccacc tgatagctta cagtttcgcc gggtgcgtca ttgcgcggta acaaacggcg
    51721 caattgtgaa aagttcatct cgcacaggcg catcatttca ggaaagtcag gtgtgtaacg
    51781 cttcatttat gcccactcat tttttaacgc ttgatgatgc agctgcagcc attgcaaagc
    51841 gatgaccgac gctgcgttgt cgattttccc ctcttctacc cactggtatg cctgttcccg
    51901 gcttaccaca tgaacgcgaa tatcttcgtt ttcatcagcc agaccatgaa taccgcttgc
    51961 ggtcgtggcg tccacttcgc ccaccataat tgacgaacgc tcactggtgc cccccgggct
    52021 tgccaggaaa cttaacaccg gtttggtccg tttgactatc agtcccgcct cttcaatcgc
    52081 ttcgcgacgg gcaacatctt ccacactttc accctcttca atcatcccgg caaccatctc
    52141 cagtagccag ggggtttcgc tggtgtcgta cgcggcaatc cgaatctgct caatcagcac
    52201 aacttcatca cgcactgggt caaagggtag caagactgcg gcgtgaccgc gctcaaaaat
    52261 ttcccgccgt acctcatgac tcatttgccc gttgaataga cgatgacgaa atctataaag
    52321 atctaatgaa aaaaagccgc gataaagtgt ttctcgtgca ataatttcta catcgttttt
    52381 gccaaatgta acgggcaggt tgtctggctt aagcattgtt aatgtcctag cactaatagt
    52441 gaattaaatg tgaatttcag cgacgtttga ctgccgtttg agcagtcata tgttaaattg
    52501 aggcacatta acgccctatg gcacgtaacg ccaacctttt gcggtagcgg cttctgctag
    52561 aatccgcaat aattttacag tttgatcgcg ctaaatactg cttcaccaca aggaatgcaa
    52621 atgaagaaat tgctccccat tcttatcggc ctgagccttt ctgggttcag ttcgttgagc
    52681 caggccgaga acctgatgca agtttatcag caagcacgcc ttagtaaccc ggaattgcgt
    52741 aagtctgccg ccgatcgtga tgctgccttt gaaaaaatta atgaagcgcg cagtccatta
    52801 ctgccacagc taggtttagg tgcagattac acctatagca acggctaccg cgacgcgaac
    52861 ggcatcaact cgaacgcgac cagtgcgtcc ctgcagttaa ctcaatccat ttttgatatg
    52921 tcgaaatggc gtgcgttaac gctgcaggaa aaagcagcag ggattcagga cgtcacatat
    52981 cagaccgatc agcaaacctt gatcctcaac accgcgaccg cttatttcaa cgtgttgaat
    53041 gctattgacg ttctttccta tacacaggca caaaaagaag cgatctaccg tcaattagat
    53101 caaaccaccc aacgttttaa cgtgggcctg gtagcgatca ccgacgtgca gaacgcccgc
    53161 gcgcagtacg ataccgtgct ggcgaacgaa gtgaccgcac gtaataacct tgataacgcg
    53221 gtagagcagc tgcgccagat caccggtaac tactatccgg aactggcggc gctgaatgtc
    53281 gaaaacttta aaaccgacaa accacagccg gttaacgcgc tgctgaaaga agccgaaaaa
    53341 cgcaacctgt cgctgttaca ggcacgcttg agccaggacc tggagcgcga gcaaattcgc
    53401 caggcgcagg atggtcactt accgactctg gatttaacgg cttctaccgg gatttctgac
    53461 acctcttata gcggttcgaa aacccgtggt gccgctggta cccagtatga cgatagcaat
    53521 atgggccaga acaaagttgg cctgagcttc tcgctgccga tttatcaggg cggaatggtt
    53581 aactcgcagg tgaaacaggc acagtacaac tttgttggtg ccagtgagca actggaaagc
    53641 gcacatcgta gcgtcgtgca aaccgtacgt tcctccttca acaacattaa tgcttctatc
    53701 agtagtatta acgcctacaa acaagccgta gtttccgcgc aaagctcatt agacgcgatg
    53761 gaagcgggct actcggtcgg tacgcgtacc attgttgatg tgttggatgc gaccaccacg
    53821 ctgtacaacg ccaagcaaga gctggcgaat gcgcgttata actacctgat taatcagctg
    53881 aatattaagt cagccctggg tacgttgaac gagcaggatt tgctggcact gaacaatgcg
    53941 ctgagcaaac cggtttccac taatccggaa aacgttgccc cgcaaacgcc ggaacagaat
    54001 gctattgctg atggttatgc gcctgatagc ccggcacccg tcgttcagca aacatccgca
    54061 cgcactacca ccagtaacgg tcataaccct ttccgtaact gatgacgacg acggggcttc
    54121 ggccccgtct gaacgtaagg caacgtaaag atacgggtta tctgccgcat tcttccccct
    54181 tctcgcttca atttcgacca gccatcctct attctgatgg gtatttacca ctggtcccgg
    54241 aagacaaaaa tgaaacggac aaaatccata cgccacgcat cgttccgcaa aaactggagc
    54301 gcacgccatc tgacaccagt cgctctcgcg gttgccactg tttttatgct ggctggctgt
    54361 gaaaagagtg atgaaacagt gtctctctat caaaatgctg acgactgttc agctgcaaac
    54421 ccaggcaaaa gcgccgaatg taccaccgcg tacaacaatg cgctgaaaga agccgaacgt
    54481 actgcgccga aatacgccac ccgtgaagac tgtgttgctg aatttggtga aggtcagtgt
    54541 cagcaagcac cagcccaggc tggcatggca ccagaaaacc aggcgcaggc tcagcaatcc
    54601 agcgggagtt tctggatgcc gctgatggcc ggttacatga tggggcgtct gatgggcggc
    54661 ggcgcgggat ttgcacagca gccgctgttc tcctcgaaaa acccggccag tccggcttac
    54721 ggtaaatata ccgacgcgac gggtaaaaac tatggcgcag cccagccagg ccgcaccatg
    54781 accgtaccga agacggcaat ggcaccaaaa ccggcgacca ccactaccgt tacccgtggc
    54841 ggttttggtg aatctgttgc caaacaaagc actatgcagc gtagcgcaac cggcacctct
    54901 tctcgttcaa tgggtggctg ataccgatgg aaagagtcag tattaccgag cgcccggact
    54961 ggcgtgagaa agcccacgaa tacggtttca attttcacac catgtacggc gagccgtact
    55021 ggtgtgaaga tgcttactac aagttgaccc tcgcccaggt tgaaaagctg gaagaagtca
    55081 ccgccgaact gcaccagatg tgcctgaaag tggtggaaaa agtgatcgcc agcgatgagc
    55141 tgatgaccaa attccgcata ccaaaacaca cctggagttt tgtgcgtcaa tcatggctga
    55201 ctcaccagcc atcgctttat tcgcgtcttg atctggcgtg ggatggcact ggcgaaccta
    55261 aacttctgga aaataacgcc gatacgccaa cgtcactata cgaggcggcg ttctttcagt
    55321 ggatctggct ggaagatcag cttaacgccg gtaacttgtc ggagggcagc gaccagttta
    55381 acagtctgca agaaaaactg atcgatcgct tcgttgagct gcgtgaacag tatggcttcc
    55441 agttgctgca tctcacctgc tgtcgcgaca cggtggaaga tcgcggaacc attcagtatt
    55501 tgcaggactg cgcaaccgaa gctgaaatcg ccaccgagtt cctctacatc gacgagatag
    55561 gtttaggcga aaaaggccag ttcacggatt tacaggatca ggtgatttct aacctgttca
    55621 agctttatcc gtgggaattt atgctgcgcg aaatgttctc caccaagctg gaagatgctg
    55681 gcgtacgctg gctggaaccg gcgtggaaga gcattatctc caacaaggca cttctaccgc
    55741 tactgtggga gatgttcccg aatcacccga acctgctgcc cgcttatttt gcggaagatg
    55801 atcatccgca aatggaaaaa tatgtggtta aaccgatctt ctcccgtgaa ggcgcaaacg
    55861 tgtcgatcat tgagaacggc aaaaccattg aagcagcgga aggtccgtat ggcgaagaag
    55921 ggatgattgt tcagcaattc cacccgttac cgaaattcgg cgacagctat atgctgattg
    55981 gtagctggct ggtgaacgat caacctgccg gaattggcat tcgtgaagac cgtgcgttga
    56041 tcacccagga tatgtctcgg ttttatccac atatttttgt tgaataagcc acgataccgg
    56101 atggcactcg ccatccggta attgttagcc tatctgcacc gacagcatac ttaaactgcc
    56161 catttctata ccatcgaccg gaatggtaat tggctcctgc ccatcccaca cacctaacac
    56221 atacaacaac ggcagatagt gctctggcgt tgggttcgat aacgcgccac cttcatggtc
    56281 gagataattc accagaggat gctgctctac cggcccttgc cacgtcagat tcgctttcac
    56341 atactcatta aacgacgtcg cccacggata cggtgaacta tcaccgtgcc acttcactgt
    56401 gcgcaggtta tgcaccacgt taccgctggc gaccaacatt attccttcat cacgcagcgc
    56461 cgcgagtttg cgccccattt cgaaatgcca ggcgacaggt ttgctactgt cgatactcaa
    56521 ctgcaccata gggatatcgg cgtcaggata catcttaatc agcacgcccc acgagccgtg
    56581 gtcaaagccc caggcttctt tatccagcgt caccgggatc ggcgctaaca gctcaaccag
    56641 acgctgtgcc agcgcaggcg aacccggagc aggataatgc gtatcgtaca gcgcctgcgg
    56701 gaagccacca aagtcatgaa tcgtgggcgg cgtctccatc gcggtcactc ctgttccacg
    56761 ggtaaaccag tgagccgaaa ccaccacaat cgcttgcggg cgtggcaatg tcatccccaa
    56821 cttctgccag ctgcgggtat acaaattatc ttccagcacg ttcatcggac taccgtgacc
    56881 taaaaacaat gctggcatac gtgttgaaga catgatgata tccttaacta aaggtgtcat
    56941 tttgatatcc tcacaatacg cttgttcggc ggagtaagaa cccggataac aatgatgatg
    57001 atcatcagtt attttgacga tctgcctgaa ggtgaagatt tataaggagt tgtcgatgtc
    57061 agtacctctc attctgacca tactggcggg ggcagccacg tttattggcg cgtttctcgg
    57121 cgttctcgga caaaaaccct cgaaccgctt actggcgttt tcgctaggtt ttgcggcggg
    57181 gatcatgttg ctcatctcat taatggaaat gcttcctgcc gcactagcag ctgaaggaat
    57241 gtcgccggtg ttgggttatg gaatgtttat cttcggtctg cttggctact ttggcctgga
    57301 ccgcatgttg ccgcatgcgc atccgcagga tttaatgcaa aaatcggtgc agctgttgcc
    57361 aaaatcgatc aagcgcacag ccattctgct cactctcggc atcagtctgc ataacttccc
    57421 ggaagggatt gccacctttg tcacggcgag cagcaacctg gagctgggat ttggcatcgc
    57481 actggccgtc gcgttgcaca atatccctga aggtctggca gtggcaggcc cggtttatgc
    57541 ggcaacgggg tctaaacgca ccgcgattct gtgggcgggg atttctggac tagcagaaat
    57601 tcttggtggt gtgctggcgt ggttaatcct cggtagcatg atttccccgg tggtcatggc
    57661 ggcaatcatg gcggcggttg caggaattat ggtggcgctc tcggttgatg aattaatgcc
    57721 gctcgccaaa gagattgacc ctaataataa ccccagctat ggcgtactat gtggaatgtc
    57781 agtgatggga ttcagtttag tgctgctaca aacggcggga attggttaaa aacaacatca
    57841 gaagttgtcc agcacatgga caacttctgc cagcttcacg aaagccttat tctacctttt
    57901 cggctgtctc ttctctcgta ctgtttaagt atttgttccg cgcttcaagc agtgcgtctc
    57961 tgtcctggcg aaacgcccga caataacgtt tcaaatgcag gaaataaccc accacaacca
    58021 gagcaatcaa tacaatccag taccaggcga taaacatcct tttaccctta atatttaaca
    58081 atactaaaca tttagcgcat aaatttcaca tatccttttt cggatatatt catatggtcg
    58141 cataataacg gaacaattat atgattttga tcataatgaa aaagaatgcc tgatattaat
    58201 gaaattttct catagaaaga gaagcggaat taacatcttg catagcaggg caaaataaaa
    58261 accgaagcca gctacaactt cggtttcttg attaaggcag taattaagca gcggttttca
    58321 gctggcttta cgctcatgtg cctgacggta tgccaccagg tcttcaatag tcacgagcgc
    58381 catattgtgt ttattggcaa actcaatgca ctctggtgca cgcgccatcg tgccatcgtc
    58441 attagtcagc tcacacagca caccagccgg tttaaagcct gccagcgtca tcaaatcaat
    58501 agtcgcttca gtatgaccgc cacgcgtcag tacaccacct gcctgcgcgc gaagtgggaa
    58561 aacgtggccc gggcgattca gatcagaagg ttttgcgcca tcggcaatcg ctgcgcgaac
    58621 ggtcgtaata cgatcagcgg cagaaacacc ggtagtcaca ccttcagccg cttcaatggt
    58681 cacggtaaaa ccggtgccat aggcgctggt gttattttct accatcattg gcagatcaag
    58741 ttgtttacgg cgatcttcag tgatgcacag gcaaacaata ccgctaccgt ggcgaatggt
    58801 cagcgccatc tgctcaacgg tcatggtttc tgccgggaag atcatatcgc cttcgttttc
    58861 acggtcttca tcatcaagca ccattacacc gcgtccttca cgcagcgcag ccagtgcatt
    58921 ttcaacacgt tcgaaaggcg taccaaaaga ggaaagtagc gtctgattca tggtaaaaaa
    58981 acctcactaa aattatggtt accagaatca gggcagtctt aggagtggcg gcatatagcc
    59041 aaaataacgt gagcgggtcc atgcccgaca gaatcgttac tctctcccat ccggactcta
    59101 accgtcggcc ccggaattac accggatctg ctgtcctttg agttcgcacc caaagcgctc
    59161 gcgggctttc aactgagttg atttaccgcc ggtggggaat ttcgccccgc cctgagaata
    59221 agcggattca ctataacgct aatgattagc ggcagcaacg catagcttca cataattctg
    59281 gtttatgact tacccttatc gcactacaat ggtactcaac acctatcagt acagggaaac
    59341 tacaatgatt gacccgaaaa aaattgagca aatcgctcgc caggttcacg aatcaatgcc
    59401 taaaggaatc agggagttcg gggaagatgt ggagaaaaaa atccgccaaa ccctacaagc
    59461 tcagctgacg cgcctcgacc ttgtaagccg tgaagagttc gacgtccaaa cgcaagtcct
    59521 gttacggact cgtgagaaac tggcgctgct tgaacaaaga atcagcgaac tggaaaatcg
    59581 cagtactgag atcaaaaagc agccagatcc agagactctc cccccaacgc tgtaaacatt
    59641 tgtctttaat gcactgtaaa ttatgaatta atcctctaat ttacagtgtt ttctattaca
    59701 ttatcgctta atcccattca gagtatcatc acaaaaatga atgattgcaa ttgaaaatat
    59761 aattgttatg tattctttaa gttttactta atcgatattt agactggccc
//
LOCUS       DAEEUE010000013        58518 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.013, whole genome shotgun sequence.
ACCESSION   DAEEUE010000013 DAEEUE010000000
VERSION     DAEEUE010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58518
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.013"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(1..1142)
                     /locus_tag="KLK84_000836"
                     /pseudo
     CDS             complement(1..1142)
                     /locus_tag="KLK84_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
     gene            complement(1354..1701)
                     /gene="pspE"
                     /locus_tag="KLK84_000837"
     CDS             complement(1354..1701)
                     /gene="pspE"
                     /locus_tag="KLK84_000837"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HBE2008283.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1744..1965)
                     /gene="pspD"
                     /locus_tag="KLK84_000838"
     CDS             complement(1744..1965)
                     /gene="pspD"
                     /locus_tag="KLK84_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HBE2008284.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1974..2333)
                     /gene="pspC"
                     /locus_tag="KLK84_000839"
     CDS             complement(1974..2333)
                     /gene="pspC"
                     /locus_tag="KLK84_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HBE2008285.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2333..2557)
                     /gene="pspB"
                     /locus_tag="KLK84_000840"
     CDS             complement(2333..2557)
                     /gene="pspB"
                     /locus_tag="KLK84_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HBE2008286.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2611..3279)
                     /gene="pspA"
                     /locus_tag="KLK84_000841"
     CDS             complement(2611..3279)
                     /gene="pspA"
                     /locus_tag="KLK84_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HBE2008287.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLRETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3446..4423
                     /gene="pspF"
                     /locus_tag="KLK84_000842"
     CDS             3446..4423
                     /gene="pspF"
                     /locus_tag="KLK84_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HBE2008288.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4543..5808)
                     /gene="puuE"
                     /locus_tag="KLK84_000843"
     CDS             complement(4543..5808)
                     /gene="puuE"
                     /locus_tag="KLK84_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HBE2008289.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5846..7126)
                     /gene="puuB"
                     /locus_tag="KLK84_000844"
     CDS             complement(5846..7126)
                     /gene="puuB"
                     /locus_tag="KLK84_000844"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HBE2008290.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7128..8615)
                     /gene="puuC"
                     /locus_tag="KLK84_000845"
     CDS             complement(7128..8615)
                     /gene="puuC"
                     /locus_tag="KLK84_000845"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HBE2008291.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8765..9322)
                     /gene="puuR"
                     /locus_tag="KLK84_000846"
     CDS             complement(8765..9322)
                     /gene="puuR"
                     /locus_tag="KLK84_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HBE2008292.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9349..10113)
                     /gene="puuD"
                     /locus_tag="KLK84_000847"
     CDS             complement(9349..10113)
                     /gene="puuD"
                     /locus_tag="KLK84_000847"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HBE2008293.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10325..11743
                     /gene="puuA"
                     /locus_tag="KLK84_000848"
     CDS             10325..11743
                     /gene="puuA"
                     /locus_tag="KLK84_000848"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HBE2008294.1"
                     /translation="METNIVEVENFVQQSEERRGSTFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11892..12056
                     /locus_tag="KLK84_000849"
     CDS             11892..12056
                     /locus_tag="KLK84_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2008295.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12046..13353
                     /gene="puuP"
                     /locus_tag="KLK84_000850"
                     /pseudo
     CDS             12046..13353
                     /gene="puuP"
                     /locus_tag="KLK84_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
     gene            13468..13713
                     /locus_tag="KLK84_000851"
     CDS             13468..13713
                     /locus_tag="KLK84_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HBE2008296.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14026..15669
                     /gene="sapA"
                     /locus_tag="KLK84_000852"
     CDS             14026..15669
                     /gene="sapA"
                     /locus_tag="KLK84_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HBE2008297.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15666..16631
                     /gene="sapB"
                     /locus_tag="KLK84_000853"
     CDS             15666..16631
                     /gene="sapB"
                     /locus_tag="KLK84_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HBE2008298.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16618..17508
                     /gene="sapC"
                     /locus_tag="KLK84_000854"
     CDS             16618..17508
                     /gene="sapC"
                     /locus_tag="KLK84_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HBE2008299.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17508..18500
                     /gene="sapD"
                     /locus_tag="KLK84_000855"
     CDS             17508..18500
                     /gene="sapD"
                     /locus_tag="KLK84_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HBE2008300.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKK"
     gene            18502..19308
                     /gene="sapF"
                     /locus_tag="KLK84_000856"
     CDS             18502..19308
                     /gene="sapF"
                     /locus_tag="KLK84_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HBE2008301.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19376..19729
                     /locus_tag="KLK84_000857"
     CDS             19376..19729
                     /locus_tag="KLK84_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HBE2008302.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20097..20885
                     /gene="fabI"
                     /locus_tag="KLK84_000858"
     CDS             20097..20885
                     /gene="fabI"
                     /locus_tag="KLK84_000858"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBE2008303.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21029..22156
                     /locus_tag="KLK84_000859"
     CDS             21029..22156
                     /locus_tag="KLK84_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HBE2008304.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETHSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22224..24158
                     /gene="rnb"
                     /locus_tag="KLK84_000860"
     CDS             22224..24158
                     /gene="rnb"
                     /locus_tag="KLK84_000860"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HBE2008305.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTTEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24236..24338)
                     /locus_tag="KLK84_000861"
                     /pseudo
     CDS             complement(24236..24338)
                     /locus_tag="KLK84_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24392..26366
                     /gene="pdeR"
                     /locus_tag="KLK84_000862"
                     /pseudo
     CDS             24392..26366
                     /gene="pdeR"
                     /locus_tag="KLK84_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26514..26687
                     /locus_tag="KLK84_000863"
     CDS             26514..26687
                     /locus_tag="KLK84_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2008306.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26777..27526
                     /locus_tag="KLK84_000864"
     CDS             26777..27526
                     /locus_tag="KLK84_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBE2008307.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27795..28013
                     /gene="osmB"
                     /locus_tag="KLK84_000865"
     CDS             27795..28013
                     /gene="osmB"
                     /locus_tag="KLK84_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HBE2008308.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28139..28465)
                     /gene="yciH"
                     /locus_tag="KLK84_000866"
     CDS             complement(28139..28465)
                     /gene="yciH"
                     /locus_tag="KLK84_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HBE2008309.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28465..29202)
                     /gene="pyrF"
                     /locus_tag="KLK84_000867"
     CDS             complement(28465..29202)
                     /gene="pyrF"
                     /locus_tag="KLK84_000867"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBE2008310.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29395..30564)
                     /gene="lapB"
                     /locus_tag="KLK84_000868"
     CDS             complement(29395..30564)
                     /gene="lapB"
                     /locus_tag="KLK84_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HBE2008311.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30571..30879)
                     /gene="lapA"
                     /locus_tag="KLK84_000869"
     CDS             complement(30571..30879)
                     /gene="lapA"
                     /locus_tag="KLK84_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HBE2008312.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31028..31792)
                     /gene="pgpB"
                     /locus_tag="KLK84_000870"
     CDS             complement(31028..31792)
                     /gene="pgpB"
                     /locus_tag="KLK84_000870"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HBE2008313.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            31962..32552
                     /gene="ribA"
                     /locus_tag="KLK84_000871"
     CDS             31962..32552
                     /gene="ribA"
                     /locus_tag="KLK84_000871"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HBE2008314.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32616..35291)
                     /gene="acnA"
                     /locus_tag="KLK84_000872"
     CDS             complement(32616..35291)
                     /gene="acnA"
                     /locus_tag="KLK84_000872"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HBE2008315.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(35455..35550)
                     /locus_tag="KLK84_000873"
     CDS             complement(35455..35550)
                     /locus_tag="KLK84_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2008316.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(35664..35831)
                     /locus_tag="KLK84_000874"
     CDS             complement(35664..35831)
                     /locus_tag="KLK84_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2008317.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(35834..35998)
                     /locus_tag="KLK84_000875"
     CDS             complement(35834..35998)
                     /locus_tag="KLK84_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HBE2008318.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            complement(36293..37267)
                     /gene="cysB"
                     /locus_tag="KLK84_000876"
     CDS             complement(36293..37267)
                     /gene="cysB"
                     /locus_tag="KLK84_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HBE2008319.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(37477..40074)
                     /gene="topA"
                     /locus_tag="KLK84_000877"
     CDS             complement(37477..40074)
                     /gene="topA"
                     /locus_tag="KLK84_000877"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBE2008320.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILCFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            40454..40705
                     /locus_tag="KLK84_000878"
     CDS             40454..40705
                     /locus_tag="KLK84_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HBE2008321.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(40741..41790)
                     /gene="sohB"
                     /locus_tag="KLK84_000879"
     CDS             complement(40741..41790)
                     /gene="sohB"
                     /locus_tag="KLK84_000879"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HBE2008322.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            42010..42768
                     /locus_tag="KLK84_000880"
     CDS             42010..42768
                     /locus_tag="KLK84_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HBE2008323.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            42765..43355
                     /gene="cobO"
                     /locus_tag="KLK84_000881"
     CDS             42765..43355
                     /gene="cobO"
                     /locus_tag="KLK84_000881"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HBE2008324.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(43395..44267)
                     /gene="rluB"
                     /locus_tag="KLK84_000882"
     CDS             complement(43395..44267)
                     /gene="rluB"
                     /locus_tag="KLK84_000882"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HBE2008325.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(44368..44988)
                     /locus_tag="KLK84_000883"
     CDS             complement(44368..44988)
                     /locus_tag="KLK84_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBE2008326.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(44985..45866)
                     /gene="yciV"
                     /locus_tag="KLK84_000884"
     CDS             complement(44985..45866)
                     /gene="yciV"
                     /locus_tag="KLK84_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HBE2008327.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            46004..46048
                     /gene="trpL"
                     /locus_tag="KLK84_000885"
     CDS             46004..46048
                     /gene="trpL"
                     /locus_tag="KLK84_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HBE2008328.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    46025..46118
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            46140..47702
                     /gene="trpE"
                     /locus_tag="KLK84_000886"
     CDS             46140..47702
                     /gene="trpE"
                     /locus_tag="KLK84_000886"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HBE2008329.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            47702..49297
                     /gene="trpD"
                     /locus_tag="KLK84_000887"
     CDS             47702..49297
                     /gene="trpD"
                     /locus_tag="KLK84_000887"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HBE2008330.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            49298..50659
                     /gene="trpCF"
                     /locus_tag="KLK84_000888"
     CDS             49298..50659
                     /gene="trpCF"
                     /locus_tag="KLK84_000888"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HBE2008331.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            50671..51864
                     /gene="trpB"
                     /locus_tag="KLK84_000889"
     CDS             50671..51864
                     /gene="trpB"
                     /locus_tag="KLK84_000889"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HBE2008332.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            51864..52670
                     /gene="trpA"
                     /locus_tag="KLK84_000890"
     CDS             51864..52670
                     /gene="trpA"
                     /locus_tag="KLK84_000890"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HBE2008333.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            53051..53230
                     /locus_tag="KLK84_000891"
     CDS             53051..53230
                     /locus_tag="KLK84_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HBE2008334.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            53316..53816
                     /locus_tag="KLK84_000892"
     CDS             53316..53816
                     /locus_tag="KLK84_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HBE2008335.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            53862..54368
                     /locus_tag="KLK84_000893"
     CDS             53862..54368
                     /locus_tag="KLK84_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HBE2008336.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(54428..55066)
                     /gene="ompW"
                     /locus_tag="KLK84_000894"
     CDS             complement(54428..55066)
                     /gene="ompW"
                     /locus_tag="KLK84_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HBE2008337.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            55306..55395
                     /locus_tag="KLK84_000895"
                     /pseudo
     CDS             55306..55395
                     /locus_tag="KLK84_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            55423..56166
                     /locus_tag="KLK84_000896"
     CDS             55423..56166
                     /locus_tag="KLK84_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HBE2008338.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            56196..56735
                     /locus_tag="KLK84_000897"
     CDS             56196..56735
                     /locus_tag="KLK84_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HBE2008339.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            56840..57238
                     /gene="yciA"
                     /locus_tag="KLK84_000898"
     CDS             56840..57238
                     /gene="yciA"
                     /locus_tag="KLK84_000898"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HBE2008340.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(57278..58006)
                     /gene="tonB"
                     /locus_tag="KLK84_000899"
     CDS             complement(57278..58006)
                     /gene="tonB"
                     /locus_tag="KLK84_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HBE2008341.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            58221..58517
                     /locus_tag="KLK84_000900"
     CDS             58221..58517
                     /locus_tag="KLK84_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HBE2008342.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT        14050 a        15133 c        14624 g        14711 t
ORIGIN      
        1 aatcagtaag ttggcagcat tacctactaa tgcaccttcc tgctgtaatg cctcgaccag
       61 cgctaatatt tcgctttctg gaagcaagcc acgcagtggg ttcaacccga tgccatcgtg
      121 agaggcgagg aagttaaacc aggtcgtttt actggagggc aagctcagac tttgcgccca
      181 ctggcacagc gcctcgacgt tctgtttttg caccgcatgt aacaccagcg gcggcagcga
      241 gaactggtac accatatgcg cttcgtcatc accgttgcca aagtaagcaa tgttgtcttt
      301 gtgcggaaca ttggtttcgg taatgatcac tgtacctggt gctacgtcat caataatcga
      361 ccgtaacagc ttgataatca gatgtgtttt ttccagatgg atgcagcttg ttcccggctc
      421 tttccacata aagccaacgg catccaggcg gacatactca gcacctttct caaggtaaca
      481 gagtaaaaca tccaccatcg ccagcaacac ttcagggcta cggtaattca ggtcaatttg
      541 atcgtcacta aaggtggtcc ataaatggcg cgtagaatta tcatccattt gaaacggcgt
      601 taatagcggt aacgcacgcg gaagggtgac ggcactgaga tcggttttcg gatcaaccgc
      661 aataaagaaa tcttcaaaac ccggaagctg ttgtaaatag tttttaaacc attcactttt
      721 tgcagacata tggttgcaga catatggttg cagacaaaat caaacattaa atgactgcat
      781 tcaccgagtt gctgaatatc ctgccactcc cccgcttcac tggcgacctg atgataatca
      841 attaccgaaa atccatcatc agatgaccat gggtaaaaag gtaataaatg aacatgtgaa
      901 aaaatgcttt gcagccattg atggtagaac tgattaaata tgggtagtgg ttttaaatca
      961 ttactgtgaa attgatcggc ataggtaatt aacacgacat cactttcatc ccagtgtttt
     1021 ttacgtcgct gtgtaattaa tcgtttcgca ctctcaagac gcgttacaag tttctgtaaa
     1081 tgagttgcgg taaatgttcc accgtagatt tcgtccatgt aatccgtaat tttctgtttc
     1141 atgttgcctt tccgtggtag cggtcccatg aggcgagaga ctactccgca gaaaataaac
     1201 tgtcaattga gcacaagggt tttagtcacg caggaaatca ttttttacca gggaaaaagc
     1261 gtatttgtga gctgacgcaa ataatctata gggatgtaaa taaggtgtga cagaaaaaaa
     1321 acggcgcata agcgccgctc atggtgaatt cttttaacct ttgaccttcg gcattgcgat
     1381 gtctttcagg ccaccggcgt tctcaacgtg ggtatatccc atctcgctaa ggagctcttt
     1441 tgcctgcccg gactggcgtc cggcattgca atacactttc acggtgtcgt ttttatccgg
     1501 aacggcggtg gcaatgcgct ctttcacttc cttcagggga atattgatgg ccccctgaac
     1561 gtgctcttgc tgatactgct ctggaacacg aacatcgatc cagtgttcag cggcgaaaac
     1621 gggcagtgaa aacaccagtg ccagagcaag taagcctttt tttaaacatg taagcgtcct
     1681 tctatggatg aaaattgcca tccattatac gcctgagatt taacaatttt tgcgaaactc
     1741 ccctcacctt ttataacgct gtgccagttt attagcagcc cgacttaaca gcggttccag
     1801 cgccacagcc agcaacattt tcagcggtcg gcgagcaact gattttatcg cccagcccgc
     1861 cacacccgcc gggccatagc gcagtgcggt aagaagtacc agcttgcctg ctaatttgaa
     1921 accaagcttc accttttgcc cggcctgttg ccagcgagta ttcataactt tcctcacagt
     1981 tgacggaaac ggctacgtaa cgtgaaagta tcggaagtga cataacgttc catctcgcgt
     2041 aaacgcgttt cacttgccgc cagttcacgg tcgacttcat ccagcaattc gctgctggaa
     2101 ggtagctgct caccaaaggc catgttgtcc ggcattggat caagcgcaaa tgacaaaatg
     2161 atgtaagcaa ccagggtaaa cagcgccaga ccgaagaaaa tcgacagcac caccaggata
     2221 cgcaccagtt ttaccggtac atcaaaatag ttggcaatcc cggcgcagac gccgcggacc
     2281 atgccctgct gtggaatacg ccataacttt ttattgagat taatgcccgc cattagcgat
     2341 ccctccagtt cggatgttct gcatcaagaa tagattccag cgcctgaata cgttcgcgca
     2401 tccgttttgc ttcatcaacc agttgcgcta atcgctgctg ctcactttgc gacaattcac
     2461 tgcgaccaga acgattgctg taatgcagcc ataaccagat cggtaaaaca aacagcacaa
     2521 aaatggttaa cggaatagcc agaaatagcg cgctcataag tactccttag acgatgagcg
     2581 gcggcgcgtt cggacgccgc ctggatgtta ttattgattg tcttgcttca ttttggcttt
     2641 taattgtgcc agttgttcgc tgattgcatc atcggctttc agttcggcaa actgatcgtc
     2701 cagcgatttt tgtttaccga agctgtggct ttctgcttcc gcttccatct ggtcaatacg
     2761 acgttcgaaa gattcgaaac gagccattgc ttcatccagt ttgccactgt ccagctgacg
     2821 acgcacatcg cgcgacgagt ttgccgcctg atgacgtaac atcaatgcct gctggcgagc
     2881 gcgtgtttct ctcaatttgt tttccagctc gccaatctct ttcttcatgc gtgccagcgt
     2941 atcgtccacc agcgtcactt catgttccag ggacttaatc agatcggtca gtttctgttt
     3001 ttcaattaac gctgcacgtg ccagatcctc tctctctttc agcagcgcca gttcggcttt
     3061 ttcctgccat tcaacttcac gcgccgacgc ttgttcaata cggcgagtca gctgtttctt
     3121 ttctgccaac gcacgcgccg aagtagaacg tacttcaacc agtgtatctt ccatctcctg
     3181 gatcatcaga cgcaccagtt tctgtggatc ttccgctttc tctaacagag cgttgatgtt
     3241 ggcattcacg atgtcggcaa agcgagaaaa aatacccata atgttgtcct cttgatttct
     3301 gcgttcagga ttgtcctgct gaactgttaa tacaatttgc gtgccaattt tttatctttt
     3361 tgatttataa agatctgatt gaagaatcaa cagcaacatg ccaggatgaa ttagcgaatt
     3421 acactaacaa gtggcgaatt tcatcatggc cgaatacaaa gataatttac ttggtgaggc
     3481 gaacagcttt ctcgaagtgc tggaacaggt ttcgcatctc gcaccgctgg acaaaccggt
     3541 gctcatcatc ggcgaacgcg gcaccggtaa agagttgatt gccagccgcc tgcattatct
     3601 ctcctcccgt tggcaagggc cgtttatttc ccttaactgc gcggcgttaa atgaaaatct
     3661 gctggattcc gaactgtttg gtcacgaagc gggggcgttt accggtgcgc aaaaacgtca
     3721 tcctgggaga tttgaacgtg ccgacggcgg tacgctattt cttgatgaac tcgctacggc
     3781 accgatgatg gtgcaggaaa aattattgcg tgtgattgag tacggtgaac tggagcgcgt
     3841 tggcggtagc cagccgttgc aggtgaatgt gcggttggta tgcgcgacga atgccgatct
     3901 cccggcgatg gtcaatgaag gtacttttcg cgctgacctg ctcgaccggc tggctttcga
     3961 tgtagtgcaa ctgccgcccc tgcgcgagcg cgaaagcgac attatgctga tggcagaaca
     4021 ctttgccatt cagatgtgtc gggaaatcaa gctgcctctg ttcccggggt ttacggagcg
     4081 cgccagagaa acattgctga attatcgttg gccgggaaat attcgtgaat tgaaaaacgt
     4141 ggtggaacgt tcagtgtatc gccacggcac cagcgattat ccgcttgatg acatcattat
     4201 tgatcccttt aaacggcgtc cacctgaaga agccatcgcc gtttcagaaa atacctcgct
     4261 tccgacgctg ccgctggatt tacgcgagtt tcagatgcag caggaaaaag agttgctgca
     4321 actcagtttg caacagggga aatataacca gaagcgcgcg gctgaattac tggggttaac
     4381 ttatcatcag ttccgcgcgt tgttgaaaaa gcaccagatt tagcgaagtt gagcaataca
     4441 acttgccgga tgcggcgtga atgctttatt cggcatacat aatcaatacg tcgtttgcag
     4501 gcctgataag cgcagcgcat cagacattat tgcgttgggc tattaatcgc tcagcgcatc
     4561 ctgcaaaatt ttcattgccg catcgaattg cgcatccggg atggtcagcg gatacaggaa
     4621 gcgaatcacg ttgccgtatg cgccacaggt cagcaggagc agcccctgcg ccagcgcgcg
     4681 ttgctggatt ttctgtgcaa tcgccgctga cggctcgccc gtttgcggat cgttaaactc
     4741 tgccgcaatc atcgacccca ggccgcgtac cgcagcaatg gccggaacgc tttctttggc
     4801 atcaatcaac gtgtttttga gacgctggcc cagttgattc gcgcgttcgc agagtgattc
     4861 tttgtcgata atattgagca ccgcgtgcgc ggcagccacc gccagcgggt tcccggcgta
     4921 ggtgccaccc aacccgcccg gcgcgggcgc gtccataata ttcgcgttac cgaccacgcc
     4981 cgaaagcggc atcccgcccg cgaggctttt cgccatcgtc attaagtccg gcttatcggc
     5041 gtaatgatcc atggcaaaca gtttaccggt acgcgcaaag ccgctttgca cttcatcggc
     5101 aatcatcaca ataccgtgct cgtcgcacag gcggcgaatg gcggcaacca gctcttttgg
     5161 cgcaacgttg aaaccgcact cgccctgcac cggttcgaaa ataatggccg cgacctgctt
     5221 cgcttcgatg tctgatttaa acaagcgttc gatggcgtcg agggagtcct gtgttgaaat
     5281 gccgtgtaaa tctgacggat aaggtacgtg atacaccgaa ccagggaacg ggccgaagcc
     5341 gattttgtac ggcgcaactt ttccggtcag cgccatggtc atatatgtac gaccgtggaa
     5401 gccaccgcta aacgcaatca cgccaggtcg tccggtatgg gcgcgagcaa ttttcaccgc
     5461 gttttccacc gcttccgcgc cggtagtgaa gaatgcggtt ttagcctgcc cgctcactgg
     5521 ggcaagggtg ttaattttct ccgccagcgt gacgtagctt tcgtacggca caatctgata
     5581 cgccgtgtgg gtaaactgtt gcaattgctg ctccaccgcc gcgaccagat cagggtggcg
     5641 atgcccggta ttcagcaccg caatgcctgc ggcgaaatcg atgtattcgt tgccctctac
     5701 gtccttcagc gtagcgtttt ctgccgactg ggcgaagaag ttacacatca cgccaacccc
     5761 gcgtggggta gcagaaagac gacgctgatg gaattcattg ttgctcatgt ttcacacctt
     5821 ttgactcttc actgttaatc ggaaattaaa cgcccagacg atcgcgcagg ctgtaataag
     5881 ccgcgcccat cgcggtaaat ggcacgcgca gcgtgcgccc gccggggaac gggtaatgcg
     5941 gcagattggc gaaggcatcg aaacgctcgg cgtcaccgcg cagcagttca gcaatcaaac
     6001 gcccggcaag atgggtacag gtgacgccgt ggccgctgta accctgcatg taatagatgt
     6061 tggtatcaag gcggccgaac tgcggcatgt gcgacaaggt cagcaggaag ttgcccgtcc
     6121 agcggtaatc aattttcacg cccttcaact gagggaaggt ttttagtagt ttcggtacca
     6181 caagacgctc aacgtcatcc gggttgcgtg caccatacac tacacccccg ccgtacagca
     6241 ggcggttgtc ggcggtaaga cggtagtaat caagcagata gttacagtct tccacacagt
     6301 agtttttcgg gatcagcgaa cgggctaaat cttccgacag ccgttcggtg gtgatcacct
     6361 gcgtgccgca cggcatgctg cgtttcgcta gttccggctc tactttatcg cccagatacg
     6421 cattcccggc gacaatcaca tacttcgccg tcacctgacc attggcagtc cgaaccacgg
     6481 ctggcgtggt gtgctggatc tgcgtcacgg cggaaagctc atacacgcgc ccgccgttca
     6541 ggcggatggc atcggcttca ccaatggcga ggtttagcgg gtgaatatgc ccaccgctgt
     6601 gatccagcag cgcaccagtg tagcgatcgc tggcgacctc gcggcgaata gcgttgacgt
     6661 ccagcagctc cagctgttta ttgccgtagc gttcccagtt ctctttctgc tcttcgagtg
     6721 tggcgagttg tttatcattc atcgccacaa acagaccgcc ggggcggtag tcgcagtcaa
     6781 tctgataacg tttgatacgt tcgcggatga tctcgccacc ctcgaacatc atgctgccga
     6841 gcatacgggc ggtgtccatg ccgtagcttt tttcgatcac gtcgatgtcg cggctgtagg
     6901 agttcacaag ctgcccgcca ttgcgcccgc ttgcgccaaa gccgatgcgt gaggcttcga
     6961 gaaccactac gtcaaagccc gcttccgcca gatgcagcgc ggaggagagc ccggtatagc
     7021 cgccgccaac cacgcaaacg tcgcaggtga ttgactcatt cagtgtgtcg aatggtgcat
     7081 atttattcgc actggcggcg tagtaactgc tggtatgttc ggtcatttca ggcctccagg
     7141 cttatccaga tggttttcag ttcagtgaat ttttcaaggg catgcaggga tttgtcgcga
     7201 ccgttgccgc tctgcttata gccgccaaac ggcacggtca tatcgccgtc gttgtagtta
     7261 ttgacgaaga cggaaccggc tttcaggcgt cggctcatgc ggtgcgcgcg ggagaggtcg
     7321 cgcgtccata ccgccgcgcc aaggccgtac tggctgtcgt tggcaagctg cagcgcctga
     7381 tcttctgatg tgaaacgggt gaccaccagc accggaccga aaatctcttc gcggcttaag
     7441 gacgcattcg ggtccacatc cacgaagatg gtcgggccga tggccgctgc cagcccggcg
     7501 ttgcgaccat ccagcagcag ttgccccttg ccttcgcctt cacgaataaa ggtgtgtacc
     7561 gagtcagcgt gagcgctgtc gattaaggtg cccatggtgg ttgcgggatc aagtggatgg
     7621 cccggctgcc agttttgcgc ctgctgtttt aacagggcta agaattcatc ggcgatgctc
     7681 tcttccagta acaaacgcgt tccggcgatg cacacctggc cctggttgta gaagatgcct
     7741 gcggcggtgg cgcttgccgc tttttgcaga tccggacaat cggcgaaaac gatgttggcg
     7801 cttttgccgc ccgcttccag ccagacacgt ttcatgttgc tatcacccgc gtctttcagc
     7861 agttgtttac ccgtacgcgt ggagccggta aaggcgatgg catcgatatc gttatgacgc
     7921 gaaagcgcct gccccgcttc atgaccaaaa cccgtcacca cgttcaacac accatccggc
     7981 aagcctgctt ctttcgccag ccccgcgaga cgaatcgcac tgagcggtga tttttcagac
     8041 ggttttagaa tcacgctgtt tcccgccgcc agcgccgggc cgagtttcca gcaagtcagc
     8101 aacagcggga agttccacgg cacgatggcg gcaatcacgc cgaccggttc acgcacgatc
     8161 atcgccagct catggctact ggtggtcgcc acttcgccat acactttgtc gatcgcttcg
     8221 gcgtaccagc gaatggcgcg cgccgcgccg ggaatatcat cacgcagact gtgacgaatc
     8281 ggtttgccgg tgtcgagagt ttccagcagt gccagctctt cggcgtgggc ttccattaaa
     8341 tcggcgagtt tattcagtac cgctttacgt ttagccggag aagagagtga ccagtcgccg
     8401 cgttcaaata cgccgcgtgc tgcgctcacc gcacggtcga tatcgacgct cttgccgcgg
     8461 gcaattttcg ccagcggtgc ctgggtgacc ggatcaacgg tttcaaaggt ttcattttcc
     8521 gccgcagcag tatattcacc gttaataaat aagcggtttt caatggcgag acttaacgct
     8581 ttatcctgcc agtaagccag atgatgaaaa ttcattatga ctcctgtttc acgtctatca
     8641 gatatatgca gtttgtgaac gtcagggcgc agagatcccc gggctgcatg cccgtttatt
     8701 tctggcgaca ttctttatgg attgccgggc gcattgcgct tacccggctt acagaacaaa
     8761 aagattaaaa cgtggtgggc gtatgggcgc tgataattcg gcaaatacct gccgacgtat
     8821 tactgaaact gtgcgggatg ccggtattaa tggcataact ttgccccgcg acgaggtggt
     8881 aatcctgacc attaatcgtc agaacaattt caccttccag tactgtgcct atttcctcac
     8941 cctgatgctt aattctttcc ccagtggttg tgcccggctg gtacgtttca aagatcatcg
     9001 ccagcgtgcg attcgggtta ccgttatgaa ccagcttcat tgacacaccc tgactgccca
     9061 tctcaattaa gtcgtcctga ttaatgacga cctgcggctc atcaggtttt tccggctcgg
     9121 aaaagaattc cgagagtgac aaaccataca ccttcagcag cttttgcagc gtactgatgg
     9181 cagggctgac tttatcttgt tctatcgtac tgatagcact gtgagtcagc ccggagagtt
     9241 cggcggcacg acgttgtgaa agcccctgct gctggcggat ttccgacaag cgttttcctg
     9301 gcgccagtcc ctcatcactc atatttgcat ttccttaaac tgtagtggtc agagtcgctg
     9361 tttttcagcg atatggtgct gccaagcggt gataaagccc tcgaacaata tacgcgaaag
     9421 cgcgtactcg ctactgttcc attccgggtg ccactgtacg cccagcgcaa aaggatgatt
     9481 gatgacgcta accgcctcaa ctaagccatc cggcgagcga gcttcaacac gcaaccgtgg
     9541 gctaacgacc ttcgcccctt gtccatgtag agagtttacc caaaagttgc tacattcagg
     9601 taacaacgca gacagtaatc ccccctcttc aacctgaact tcatgcgacg gtgcatattg
     9661 ctgttccacc ggcagttcgg gatcttcccg atgttccagc aattcaggct gttcgcacag
     9721 cttgcgatgc aacgaaccac cggttgccac caccagttct tgtaaacccc ggcagatggc
     9781 gaaaatgggg atgcgccttt cgagtgcggc attaattatc gccatgctca gaagatcacg
     9841 cccgggatcg gcgtcaggct catcgccgtt ttcaccatat aggtgcggct gcacattgct
     9901 gggactacta ggaagataaa tgccatcgag tttcggcaaa agttgttcaa gtaacgacgg
     9961 ttccgccagc gcatgtggta gcgcaattgg caagccgcct gcgtggatga tggcattcag
    10021 gtacttttct tgcagagtct gggtcgcatg acccttaagc ctgttcctgc acattacgac
    10081 accgataacc ggattgttca ttatattttc catgctcaat ctcacaaagt ggactaaatt
    10141 atcgccatta ctgcgcttta aatgaccttt atgttcaata ttttttcaat ctagcagtgg
    10201 attttcattt ttgcaaactc aatttaacat ttgacaaaca tttggtttgc atacagattc
    10261 gaatgatggt cattatattt tacgctttga taacgagcgg aaaacaaacc aaaggcgaag
    10321 aatcatggaa accaatatcg ttgaagtaga gaactttgtt cagcagtcag aagagaggcg
    10381 gggtagcacc tttacgcagg aagtgaaacg ctacctggag cgctacccga atacgcaata
    10441 tgttgatgtt ttacttactg atttgaatgg ttgcttccgt ggaaaacgta tccctgtttc
    10501 aagcctgaag aagctcgaga aagggtgtta tttcccggcc tcggtatttg caatggatat
    10561 tctgggcaac gtagtagaag aagcaggtct gggtcaggaa atgggcgagc cggatcgtac
    10621 ctgtgttcct gttctcggtt ccttaactcc ttctgccgcc gatccagagt ttatcggtca
    10681 gatgctcctg accatggtcg atgaagatgg cgctcccttt gacgttgagc cgcggaacgt
    10741 tcttaaccgc ctgtggcagc agctgcgcca gcgcggattg ttcccggttg tagcggtaga
    10801 gctggagttc tatttactgg atcgccagcg cgacgctgaa ggggatctgc aaccgccctg
    10861 cgcgcctggc accgatgacc gcaatacaca aagccaggtt tactccgttg ataacctcaa
    10921 ccacttcgcc gacgtgctca atgatattga tgaactggcg cagttgcagc tgattccggc
    10981 agatggcgcg gtcgctgagg cctcgccggg tcagtttgaa atcaaccttt accatactga
    11041 taacgtgctg gaagcctgcg atgatgcgct ggcactaaaa cgcctagtgc gtctgatggc
    11101 agaaaagcat aagatgcacg ccacttttat ggcgaagccg tatgaagagc acgcgggcag
    11161 cgggatgcat atccatatca gtatgcaaaa taatcgtggc gagaatgtgc tttctgacac
    11221 agaaggcgaa gattcgccgc tgctgaaaaa gatgctcgcc gggatgattg acctgatgcc
    11281 gtcgtcgatg gcgttgctgg caccaaacgt gaactcgtat cgccgcttcc agccgggaat
    11341 gtatgtgccg acgcaggcgt cgtggggcca taacaaccgc accgtcgccc tgcgtattcc
    11401 gtgcggtgac cgtcataatc accgcgtgga atatcacgtg gcgggtgccg atgccaaccc
    11461 atatctggtg atggcagcga tttttgccgg tattttgcat ggccttgata acgagctgcc
    11521 gttgcaggaa gaagtcgaag gcaacgggct ggaacaggaa ggcttaccct tcccgattcg
    11581 ccagagcgat gccctgggtg agtttatcga gaatgatcac ctgcgccgct atttaggcga
    11641 acggttttgc catgtgtatc acgcctgtaa aaatgatgag ctgttgcagt ttgagcgcct
    11701 catcacagaa acagaaattg agtggatgtt gaaaaacgcg taattcccct tgcggggttg
    11761 tttcatcggc ctgagcggag ccaggcctga catttgctgg taatccagac atttcccgga
    11821 tgtcgcgtta tggagcgcag gcggcaacgg gcttccgtta acgattaaga ctgtgacgag
    11881 gaaatgagat gatgccgatg attaaatccc cgcatggcga gggaggctgc gtatgcgccc
    11941 ctcctgctac tgactggact ccgccccctt tgcttccctt gctaaacagg tttgatttca
    12001 ggtcgacacg accgcaaacc ttattacgca ggggaggcag caattatggc tattaattca
    12061 ccactgaata ttgctgcgca acccggcaaa acccgtctgc gaaaatcact gaaattgtgg
    12121 caggtggtaa tgatgggtct ggcctatctc acgccgatga ccgtatttga tacttttggt
    12181 attgtctccg gcattagcga cggtcacgtt ccggcgtcct atttgctggc gctggcgggc
    12241 gtgctgttta ccgctatcag ctacggcaaa ctggttcgcc agtttccgga ggccggttcg
    12301 gcctatacct acgcgcaaaa gtcgattaac ccgcacgtcg gatttatggt cggctggtca
    12361 tcactgctgg attatctctt tttgccaatg atcaacgttt tgttggcgaa aatctatctc
    12421 tccgccctct tcccggaagt gccgccgtgg gtgtgggtgg taaccttcgt cgccatttta
    12481 accgccgcga atctgaagag cgtcaacctg gtcgctaact tcaataccct gtttgtactg
    12541 gtgcaaatct ccatcatggt ggtgtttatc ttcctggtgg ttcagggact gcataaagga
    12601 gaaggcgttg gcaccgtctg gtcacttcag ccgtttatca gcgagaacgc gcacctgatc
    12661 ccgattatta ccggtgcgac gattgtctgt ttctcgttcc tcggtttcga tgcggtgacc
    12721 acgctttcgg aagagacgcc ggacgccgca cgcgtgatcc cgaaagccat cttcctgacg
    12781 gcggtatacg gcggcgttat ctttatcgcg gcgtcgttct ttatgcagct gttctttccc
    12841 gatatcagcc gctttaaaga cccggacgcc gcactgcctg aaattgcgct gtacgtcggc
    12901 ggcaagctct ttcagtcgat tttcctctgc accacgtttg tgaacacatt agcgtctggc
    12961 ctggcttcac atgccagcgt gtcgcgtctg ctgtatgtga tggggcgtga caatgtgttt
    13021 ccggagcgcg tgtttggcta tgtgcaccca aaatggcgga ctccggcact caacgtcatt
    13081 atggtcggga ttgtcgcgct gtcggcgctg ttctttgatt tagtcaccgc gacagcactg
    13141 attaacttcg gtgctctggt ggcgtttacc ttcgtgaatc tgtcggtgtt taatcatttc
    13201 tggcggcgta aaggaatgaa taaaagctgg aaggatcact tccactattt gctgatgccg
    13261 ctggttggcg cgctgacggt gggtgtgctg tgggttaacc tagagtcaac gtcactgaca
    13321 ctcggtctgg tatgggcttc gttaggcggc gcattatcct gcctgagggt aacgcgccgc
    13381 gccattaact ataaaaagat gtatgacccg gatcacgagt gctatcatcc gcccttatgc
    13441 ttagcgcatt aaccccaggg gcaaataatg aaccacgata tcccgctaaa atattttgat
    13501 attgccgatg agtacgcgac cgaatgcgca gagcctgtcg cagaggctga acgcacaccg
    13561 ctggcccact acttccagtt actgctcacc cgtttgatga acaatgaaga gatcagcgaa
    13621 gaagcccaac atgaaatggc tgctgaagcg gggattaatc ccgtacgcat tgatgagatc
    13681 gcagagtttt tgaatcaatg gggtaatgag taattattta attgcagacg atcggtcagt
    13741 atcgttgtcc ggataaagcg ttcacgccgc atccggctaa caatgacacg cactgcagcg
    13801 tgctttaatg ggtttctcac gatcgtaccg ctctgctcct cgctaagttg ttctgtgagt
    13861 tatagttata gagaaatcac gggtacgaat gttgaacaga tggcgacgcc acctgttcaa
    13921 cgaccaacca cacggccctc attacgaccc ctgaacacaa acggtaatgg caaatgtcat
    13981 taaatattag taagatagcc tctttacata aaatccctta atattatgcg ccaggtatta
    14041 tcgtctcttt tggtgattgc tggacttgtg agtggtcagg caatcgccgc gcctgaatcc
    14101 cccccgcatg ctgatatccg cgacagcggt tttgtctatt gcgtcagcgg gcaagtcaac
    14161 acctttaacc catccaaagc gagcagtggg ttaattgtcg atacccttgc cgcccagttt
    14221 tatgatcgac tgctggatgt cgatccctat acctatcgcc tgatgcctga acttgccgaa
    14281 agctgggaag tactcgacaa cggcgcgacc tatcgcttcc acctgcgtcg cgatgttccg
    14341 tttcaaaaaa ccgactggtt tactcccact cgtaaaatga atgccgacga tgtggtgttt
    14401 accttccagc gaatttttga ccgcaacaac ccgtggcata acgtcaacgg cagcaacttc
    14461 ccctacttcg acagcctgca atttgccgat aacgtgaaaa gcgtccgcaa actggataat
    14521 cataccgttg agttccgact cgcccagccg gatgcttctt ttttgtggca cctcgcaacc
    14581 cattatgctt cggtcatgtc ggcagaatat gcccggaagt tagagaaaga agatcgccag
    14641 gagcaactcg accgtcaacc ggtcggcacc ggtccgtatc agttgtcgga ataccgcgcc
    14701 gggcaattta ttcgcctaca acgtcatgat gacttctggc gcggtaaacc gttaatgccg
    14761 caggtagtgg tggatttagg ctccggcggc accggacgtc tgtcgaaact cctgaccggg
    14821 gaatgcgacg ttctggcctg gcctgctgcc agccagctat ccattttgcg tgacgacccg
    14881 cgcttgcgtt taacgctgcg tcctgggatg aacgtcgcct atctggcatt taacaccgcc
    14941 aaaccgccgc taaataatcc cgctgtccgc catgcactgg cactggcgat taataaccag
    15001 cgcctgatgc aatccatcta ttatggtacg gctgaaacgg cggcctctat tttaccgcgc
    15061 gcctcgtggg cctatgacaa cgaggctaaa attactgaat acaatccggc gaaatcgcgc
    15121 gaacagttga aggcgttggg gctggaaaac ttaacgctga aactgtgggt gcccacacgt
    15181 tcgcaggcgt ggaaccccag tccactgaaa actgccgaac tgattcaggc ggatatggcg
    15241 caggttggcg taaaagtggt gattgtgccg gtagaaggtc gctttcagga ggcgcggttg
    15301 atggatatga gccatgatct gacgttatcc ggttgggcga cggacagtaa cgacccggac
    15361 agtttcttcc gtccgttact gagctgcgcg gcaattcatt cacagaccaa cctcgcccac
    15421 tggtgcgatc cgaaattcga cagcgtgttg cgtaaggcgc tctcctcgca gcagctggca
    15481 gcgcgtattg aagcctatga cgaagcgcag agtattctgg cgcaggaatt acctattctg
    15541 ccgctggcgt cgtcattgcg tttgcaggcc tatcggtacg atatcaaagg tctggtactt
    15601 agcccgtttg gtaacgcctc ctttgctggg gtgtatcgcg agaaacagga tgaggtgaaa
    15661 aaaccatgat tatcttcacc ttacgccgca ttttgttatt gattgtcacc ttgttcctgc
    15721 tgaccttcgt tggcttcagc ttgagctatt tcactccaca cgcgccgtta caaggcgcgt
    15781 cactgtggaa tgcctgggtg ttctggttta atggcctgat ccactgggat tttggtgttt
    15841 ccagcattaa cggtcaacca attgccgaac agttaaaaga gctcttcccg gcgacgatag
    15901 aactgtgcat cctcgccttc ggttttgcgc tgattgttgg catccccgtg gggatgattg
    15961 ccgggattac gcgccataag tggcaggata acttgatcaa cgccatcgcc ttactgggct
    16021 tttcaatccc ggtgttctgg ctggcgcttc tgttgacact gttttgttca ctcacgctag
    16081 gttggttgcc cgtttcaggg cgtttcgatc tgctctacga agtgaaaccg attaccggtt
    16141 ttgcgttgat tgatgcctgg ctttcggact caccatggcg ggatgaaatg atcatgagcg
    16201 caatccgcca tatgatattg cccgtgatca ccctgtcggt tgcgccaaca actgaagtga
    16261 tccgcctgat gcgtatcagc actattgaag tgtatgacca gaactatgta aaagcggcgg
    16321 cgacccgcgg tttgtcgcgc tttactattt tgcgtcgcca cgttctgcat aacgcgttac
    16381 cgccggttat tcctcgcctg ggcttacagt tttccaccat gctgacgctg gcgatgatca
    16441 ccgaaatggt ttttagctgg ccgggtttag ggcgctggtt aattaacgcc atccgccagc
    16501 aggattacgc agccatttcc gccggagtga tggtgtgtgg ctcactggtc attattgtta
    16561 acgtgatttc tgatattttg ggtgccatgg ctaaccctct gaaacataag gaatggtatg
    16621 ccttacgata gcgtatacag cgaaaagcgc ccgccgggca cgctgcgtac cgcctggcgc
    16681 aaattttata gtgacgcctc tgcgatggtc ggcctgtacg gctgcgcggg actggctgta
    16741 ctgtgtattt ttggcggctg gtttgcgcct tacggcatcg accagcaatt tctcggttat
    16801 caattattgc cgccatcatg gtcgcgctat ggcgaagttt ctttcttcct ggggactgac
    16861 gacctcggac gtgatgtttt aagccgttta ctgagcggag ctgcgccaac cgtgggtggc
    16921 gcatttgtgg tgacgcttgc cgcgacgatc tgtggcctgg tactgggcac cttcgccggg
    16981 gcgacacacg gcctccgctc agcggtgctt aaccatattc tggatacctt actggcgatc
    17041 ccttcgctgc tgttggcaat tatcgttgta gcgtttgccg gaccgagttt gtcccacgct
    17101 atgtttgctg tctggctggc attgctgccg cgtatggtac gttcgattta cagcatggtg
    17161 catgacgaac tggaaaaaga gtacgttatc gccgcccgtc tggatggcgc atcaacgctg
    17221 aatattctct ggtttgctgt gatgccaaac atcaccgccg ggctggtcac cgagatcacc
    17281 cgcgcactgt cgatggcaat tctcgatatc gccgcgctgg gctttctcga tctcggcgca
    17341 caactcccct cgcctgaatg gggagcgatg ctcggtgatg cgctggaact gatttatgtc
    17401 gccccgtgga ctgtcatgct gccaggtgcg gcaattatga ttagcgtgtt gttggttaac
    17461 ctccttggcg atggcgttcg tcgtgcgatt attgcggggg tggaataatg ccattactcg
    17521 atattcgtaa cctgaccatt gaatttaaaa ccggtgatga gtgggttaaa gccgtcgacc
    17581 gggtaagtat gaccttaacc gaaggtgaaa tccgcggtct tgttggtgaa tccggttccg
    17641 gcaagagttt gattgcgaaa gcgatttgtg gggtgaataa agataactgg cgtgttactg
    17701 ctgaccgtat gcgttttgat gatatcgatt tgctgcgtct ctccgcacgc gaacggcgca
    17761 aactggttgg ccataacgtg tcgatgattt tccaggaacc gcagtcgtgt cttgaccctt
    17821 cagaacgtgt gggtcgccag ttgatgcaaa acatcccagc ctggacctat aaaggccgtt
    17881 ggtggcagcg ttttggctgg cgcaaacgcc gtgccattga actgctgcac cgcgtgggga
    17941 ttaaagatca caaagatgcg atgcgcagtt ttccctatga gttgaccgaa ggtgaatgtc
    18001 agaaagtgat gatagccatt gcactggcga atcaaccgcg tctgctgatt gctgacgaac
    18061 cgacaaactc aatggagcca acaacccagg cgcaaatctt tcgcctgctg acgcgtctca
    18121 accaaaacag caataccact attttgctta tcagccatga cttacaaatg cttagccaat
    18181 gggcggataa aattaacgtg ctttactgcg gtcaaacagt ggaaaccgcg ccgagtaagg
    18241 agttggtgac gatgccacat catccttata cccaggcgct gatccgcgcg ataccagact
    18301 tcggcagcgc gatgccgcat aaaagtcgcc tcaatactct acccggcgct atcccattgc
    18361 tggaacagtt accgattggt tgtcgtctgg ggccacgttg cccgtatgca caacgagaat
    18421 gcattgtgac gccacgtttg acgggggcga aaaatcatct ctatgcctgt catttcccgc
    18481 tgaacatgga gaaaaagtga gatgatcgaa acgctgcttg aagtgcgtaa tttaagtaaa
    18541 acgttccgct accggaccgg ttggtttcgt cgtcagaccg tagaagcggt aaaacccttg
    18601 agctttacgc tacgtgaagg ccagacactg gcgattattg gcgagaatgg ttcgggtaaa
    18661 tccacactgg caaaaatgct ggcgggaatg atagaaccta ccagcggcga gttattgatt
    18721 gacgatcatc cgctgcattt tggcgattat tccttccgta gtcagcgtat tcgcatgatt
    18781 tttcaggatc cttcgacctc gttgaatccc cgtcaacgta tctcacaaat tctggatttt
    18841 ccactgcgcc tgaacaccga cctggaaccg gaacagcgtc gcaaacagat tattgaaacg
    18901 atgcgtatgg ttgggctgct accggatcac gtcagttact atccgcatat gttggcaccc
    18961 ggacaaaaac agcgtctggg tctggcgcgc gcgctgatat tgcgcccaaa agtcattatt
    19021 gccgatgaag ccctcgcttc actggatatg tcaatgcgtt cgcagttgat taatctgatg
    19081 ctggagttac aggaaaaaca gggcatttcg tatatttatg ttacccagca tatcggaatg
    19141 atgaagcaca ttagcgacca ggtgctggtg atgcatcagg gcgaggttgt cgagcgaggc
    19201 agcaccgcgg atgtgctggc atcgccgctg catgaactca ccaaacggct gattgccggg
    19261 cactttggcg aagcattgac agcggatgcg tggcgtaaag atcgctaatt ttctgaatcc
    19321 tccttccaga ttattcacct cattctgaca agagattaaa tcttgtcaga atgaggtgat
    19381 ggataaaata aaatcaaatg cgcgtgattt aagacgcaat ctcactttgc aggaacgaaa
    19441 gctctggcga tatcttcgca gccgacgttt tggtgatttc aaatttcgcc gtcaacatcc
    19501 ggtggggagc tacattctcg attttgcttg ctgctcggcg cgcgtagtcg ttgagctgga
    19561 tggtgggcag catgatttgg cagttgccta tgataccagg cgcactagct ggcttgagtc
    19621 gcagggctgg accgtgctgc gtttctggaa taacgagatt gattgtaatg aggaggcggt
    19681 gctggagatt attctgcagg aactgaaccg ccggtcaccc tcaccctgaa agggcgaggg
    19741 ggccgaccga gccgaatagc tgttgtggtg aaaacatgga gacagtgctg gagaatattc
    19801 ggcaaggtct gaaccatccc agccatcgcc ataaaagggt taggggctgt atgagcctgt
    19861 ttgttgctgg ggtaacaata tttgcacaat atggtcccct cgcccctctg gggagagggt
    19921 tagggtaagg ggaaaagcgc cccccctgcc gcagcctgct ccggtcggac ctggcaacta
    19981 tagctactca cagccaggtt gattataata accgtttatc tgttcgtact gtttactaaa
    20041 acgacgaatc gcctgatttt caggcacaac aagcatcaac aataaggatt aaagctatgg
    20101 gttttctttc cggtaagcgc attctggtaa ccggtgttgc cagcaaactg tccatcgcct
    20161 acggtatcgc tcaggcgatg caccgcgaag gagctgaact ggcattcacc taccagaacg
    20221 acaaactgaa aggccgcgta gaagaatttg ccgctcaatt gggttctgac atcgttctgc
    20281 agtgcgatgt tgcagaagat gccagcatcg acaccatgtt cgctgaactg gggaaagttt
    20341 ggccgaaatt tgacggtttc gtacactcta ttggttttgc acctggcgat cagctggatg
    20401 gtgactatgt taacgccgtt acccgtgaag gcttcaaaat tgcccacgac atcagctcct
    20461 acagcttcgt tgcaatggca aaagcttgcc gctccatgct gaatccgggt tctgccctgc
    20521 tgaccctttc ctaccttggc gctgagcgcg ctatcccgaa ctacaacgtt atgggtctgg
    20581 caaaagcgtc tctggaagcg aacgtgcgct atatggcgaa cgcgatgggt ccggaaggtg
    20641 tgcgtgttaa cgccatctct gctggtccga tccgtactct ggcggcctcc ggtatcaaag
    20701 acttccgcaa aatgctggct cattgcgaag ccgttacccc gattcgccgt acagttacta
    20761 ttgaagatgt gggtaactct gcggcattcc tgtgctccga tctctctgcc ggtatctccg
    20821 gtgaagtagt ccacgttgac ggcggtttca gcatcgctgc aatgaacgaa ctcgaactga
    20881 aataatcgtt ctgttggtaa agatgggcgg cgttctgccg cccgttatct ctgttatacc
    20941 tttctgatat ttgttatcgc cgatccgtct ttctcccctt cccgccttgc gtcaggataa
    21001 cgatttcctt tacgaccaag gagcgcccat ggaacaacgc cacatcaccg gcaaaagcca
    21061 ctggtatcat gaaacgcaat ccagtactac ggagtatgac gttctgcctc tggtcccgga
    21121 agccgcaaag gtcagcgatc cctttctgct cgacgtgatc cttgatgaag agacgctggc
    21181 ccccttcctt tcatggctgg tccctgcgcg cgttcttgca gtggagttgt tccctgacca
    21241 gcttaccgtg acccgttcac aaaccttcac cgcttatgaa cgcttgtcga cggccctgac
    21301 ggttgctcag gtttgcggcg tccagcggtt atgtaactac tattcggcgc gacttacgcc
    21361 gctccccgga cctgattcct ccagggaaag taatcatcgg ttggcacaaa tcacgcaata
    21421 tgcccgccaa ctggctagct cgccttctat tatcgacaac cgatcgcgcc agcatctgaa
    21481 tgacgtcggt cttactgtct gggactgtgt gatcattaac caaatcattg gttttattgg
    21541 ctttcaggcg cggacaattg cgacatttca ggcttatctc gggcatccgg tacgctggtt
    21601 acccgggctg gcgatacaaa actacgccga cgcgtcactg tttgctgatg aatcaatacg
    21661 ctggcgaagc agctatgagg tggaaaaact acctgaagag cacacaaaaa gttcaactgc
    21721 agaactttgc caactggccg aaacacactc tctccaccct atttcacttt cccttctcga
    21781 aaagttgtta aacagcacac gggtcaatgc acagccggat aatcagcttg cggcgttgtt
    21841 atgcgcgcgt ataaatggca gtcctgcttg tttttccacc tgtatggatt catcaaatga
    21901 atataaaaaa atcagcaccc ttctgcgcaa gggcgaaaat gaaattaacc gatgggctga
    21961 ccgtcattct gttgagcgcg ctaccgttca ggcgatacaa tggctgaccc gagcacccga
    22021 tcgctttagc gccgcccagt tcagcccttt actcgaacac gaaaaatcat caacgcagat
    22081 tattaatctg ctggtatgga gcgggctgtg tggctggata aatcgcttaa aaatcgcgtt
    22141 gggtgagaca tattaacctt gccgcgtcag acagattcgc gtaaaactgt cagccgctct
    22201 aatggccacc aaaatagaca attatgtttc aggacaaccc gctgctagcg cagcttaaac
    22261 agcaactgca ttcccagacg ccacgcgctg aaggggtggt aaaagccaca gaaaaaggct
    22321 ttggcttcct ggaagtcgac gcacaaaaaa gttatttcat tccgccgccg cagatgaaaa
    22381 aagtcatgca tggcgaccga attatcgcgg tgatccacag tgaaaaagaa cgtgaatccg
    22441 cagagccaga agaactggtt gaaccgttcc tgactcgttt cgtgggtaaa gttcagggca
    22501 aaaatgaccg tctgaccatc gttcctgatc atccactttt aaaagacgcc attccttgcc
    22561 gcgcagcccg tggcctgaac cacgagttta aagaaggcga ctgggcggtt gccgaaatgc
    22621 gccgtcatcc gctgaaaggc gatcgttctt tctatgctga actgacacaa tacatcactt
    22681 ttggtgacga ccactttgta ccgtggtggg ttacccttgc gcgccataat ctggaaaaag
    22741 aagcaccaga cggcgtcgct accgaaatgc tcgatgaagg tctggttcgt gaagatctga
    22801 cctcgctgga ttttgtcacc atcgacagtg ccagcactga agatatggat gacgcccttt
    22861 tcgctaaggc gctgccggat gacaaacttc agctgattgt ggcgattgcc gatccaaccg
    22921 cgtggattgc tgaaggcagc aagctggaca aagccgcgaa aattcgcgca ttcaccaact
    22981 atctgcctgg cttcaacatc cctatgctgc ctcgcgagct ttctgacgat ctctgctcac
    23041 tgcgcgccaa tgaagtccgc ccggtactgg catgccgcat gacgctctcc gctgatggca
    23101 ccactgaaga taatatcgaa ttctttgccg ccaccatcga atccaaagcg aagctggtgt
    23161 atgaccaggt ttctgactgg ctggaaaata ccggtgactg gaagcctgaa agtgaagcaa
    23221 ttgccgaaca agtccgtttg ctagcgcaaa tttgccaacg ccgcggcgag tggcgtcata
    23281 accacgcact ggtgtttaaa gatcgcccgg attaccgctt tattctcggt gaaaaaggtg
    23341 aagtgctgga tatcgtcgcc gagcctcgtc gcattgccaa ccgtatcgtc gaagaagcga
    23401 tgattgccgc taacatttgt gcggcccgcg tactgcgcga taagctcggt tttggcatct
    23461 ataacgtgca tatgggcttt gatccggcga atgccgacgc gctggcagcg ttgctgaaaa
    23521 cgcacggtct gcatgtcgat gccgaagaag tgctcacgct ggacggtttc tgcaaactgc
    23581 gtcgtgaact ggatgcgcaa ccaactggtt tcctcgacag ccgcattcgt cgcttccagt
    23641 catttgctga aattagctct gaacccggtc ctcactttgg cctcggtctg gaagcatacg
    23701 ccacctggac ttcgccgatc cgtaaatatg gcgacatgat caaccaccgt ctgctgaaag
    23761 cggttatcaa aggcgaaact gcgacgcgtc cgcaggatga aatcactgtc caaatggccg
    23821 aacgtcgccg tctcaaccgg atggcagaac gtgatgttgg cgactggtta tacgcacgct
    23881 tcctgaaaga taaagccggg accggcaccc gtttcgcggc ggaaattgtc gatatcagcc
    23941 gtggcggcat gcgtgtgcgt ttggttgata acggcgctat cgcctttatt ccggcacctt
    24001 tcttacacgc tgtgcgcgat gaactggttt gcagccagga aaacggcact gtacaaatta
    24061 aaggtgaaac ggtttacaaa gtaactgacg ttattgacgt caccattgcc gaagtccgca
    24121 tggaaacccg cagcattatt gcgcgcccgg tcgcgtaatc tcctttcacg gcccattcct
    24181 catggatggg ccgtttattt ccccgctcta ccttcgttat atccaggcag attttttaac
    24241 tgactttcgt ttgaaaactg gcgtttttcc aaaactggat tacaaataaa tatataaaaa
    24301 acaatgcgtt tagccgccgt taatttcatg taagtcatgt tgttcctttt atttaaccgc
    24361 ggactccgct gttaaccgga ggatatgcat catgaaaacc gttagggagt ccacaacgtt
    24421 gtacaacttt ctcggatcgc acaatccata ctggcggttg acggaaagca gcgatgcttt
    24481 gcgcttttct accaccaaaa ccacagaacc tgatcgtaca ttgcagttat ctgccgaaca
    24541 ggctgctcgc atcagggaaa tgacggtcat cacctccagc ctgatgatga gtctgaccgt
    24601 cgatgaaagc gatctttctg tgcatctggt aggacgaaaa atcaataaac gggaatgggc
    24661 tggcaacgcg tctgcatggc atgacacacc ggcggttgct cgtgatttat cacacgggct
    24721 ttcctttgct gagcaggtag tttctgaagc acattccgca atagtgattc tcgacagccg
    24781 ggggaatatc caacgcttca atcggttatg tgaagattac acagggttga aagaacacga
    24841 cgtcattggg taaagcgtgt ttaaactgtt tatgagccgt cgtgaagctg cggcatccag
    24901 gcgcaataac cgtgtatttt ttcgaagcgg caatgcatat gaagtcgaac tgtggatacc
    24961 aacacgtaaa ggccagcggc tgtttctgtt tcgcaataaa tttgtccaca gcggcagtgg
    25021 caaaaacgag atttttttaa tctgttccgg taccgacatt accgaagagc gccgcactca
    25081 ggagcgactg cgtattctgg caaataccga cagtatcacc ggactgccga atcgtaacgc
    25141 aatgcaggag ttaatcgatc acgctattaa tcaggcagat aacaataaag ttggggttgt
    25201 gtatcttgat ttggataatt ttaaaaaggt caacgacgcc tatgggcatt tgtttggtga
    25261 ccagttatta cgcgacgtgt cattggctat tttaagctgt ctcgaacatg accaggtgtt
    25321 ggcgcgtcca ggtggcgatg agtttctggt actggcatcc aatacctcac aaagcgcgct
    25381 ggaagcaatg gcatcacgaa ttttgacccg cttacgcctc ccctttcgta ttggtttaat
    25441 tgaagtttat accagctgtt cagtaggtat ttcgctctct cccgaacatg gttcggacag
    25501 cgcgactatt attcgtcacg ccgacacagc aatgtacaca gcgaaggaag gcggacgagg
    25561 acaattttgc gtttttactc cagaaatgaa tcagcgggta tttgaatatc tctggctgga
    25621 taccaacttg cgtaaagcac tggaaaacga tcagttggtt atcgaaaatc acctagcgtg
    25681 gcgaagtgcg cagtctggaa gcactagtac gttggcagtc acctgaacgt gggttgattc
    25741 caccgttgga cttcatttcc tacgccgaag agtcagggct aattgtgcct ttaggccgtt
    25801 gggtgattct cgatgtcgta cgccaggtgg caaagtggcg ggataaaggc ataaacctgc
    25861 gagtggcggt aaatatttct gcacgtcagc tcgccgatca aaccattttc accgccctga
    25921 aacaggttct ccaggaactc aattttgaat actgccctat agatgttgaa ctgacagaga
    25981 gttgtctgat tgagaatgat gaactggcac tgtctgttat tcaacaattt agccaactag
    26041 gtgcgcaagt gcatctggat gattttggta ccggctactc ttcactttcg caactggcgc
    26101 gctttccgat cgatgccatc aaacttgacc aggtttttgt tcgagatatt cacaaacaac
    26161 ctgtctcgca gtcactggtc cgggcgatcg tcgctgtggc ccaggcattg aatcttcagg
    26221 tgatcgccga aggtgtagag agtgcaaagg aagatgcttt tttaaccaag aacgggatca
    26281 atgagcggca aggatttttg tttgccaaac cgatgcccgc cgtcgccttc gaacgctggt
    26341 ataaacgcta tctgaagcgc gcataagtag atgaatatat atacttagcg ctatatcccg
    26401 tcattatata tcgtattcct ggatgataat tagccgttgc tgttaaacac ttctctttta
    26461 gcggcaattt ttgcattatt cattttcccc cttttgctat aaggatgtcc ttgatgtctg
    26521 aatttgatgc ccagcgcgtt gccgaacgta ttgatattgt gttagatatc cttgtggcag
    26581 gcgattacca ctctgcgatc cataatctcg agatacttaa agcagaactc ttgcgccagg
    26641 ttgctgaatc tacgccagac atcccgaaga caccgtggga gatttaacaa tcgtgccttc
    26701 aggttttcct atttgaggat gcccggcgcg tcagtatttt tttatttagt attataacgt
    26761 tataagaatt acagcgatga attcccgaca acaaactatt ctacagatgg tcattgacca
    26821 gggtcaggtt agcgtaaccg atctggcaaa agccactgga gtttctgaag ttaccattcg
    26881 ccaggatctc aacaccctcg aaaaactgag ttacctccgc cgtgcacatg gctttgcagt
    26941 ttcgcttgat agtgatgacg tcgaaacccg tatgatgagc aactatacgc tgaagcgtga
    27001 actcgccgag tttgccgcgt cactggttca accgggcgaa accatcttta tcgaaaatgg
    27061 cagcagcaat gccctgctcg cccggactct gggggagcag aagaaaaatg tcactatcat
    27121 cacggtcagc agctacatcg cgcatttgct gaaagacgcg ccttgtgaag tcattttgct
    27181 cggtggcgtg taccagaaaa aaagcgaaag tatggttggc cctttgacac gccagtgcat
    27241 ccagcaggta catttcagca aagcatttat tggtattgat ggctggcaac ctgaaactgg
    27301 attcaccggt cgcgatatga tgcgcaccga tgtggtcaat gccgtgctgg aaaaagagtg
    27361 cgaagcgata gtcctgactg acagctcgaa atttggtgct gtacattcat actccatcgg
    27421 tcccgttgag cgattcaatc gcgtgattac cgattcgaaa atacgcgcca gcgatctgat
    27481 gcatcttgag cacagtaaac tcaccgttca cgtcgttgac atttaaccat ttcccttctc
    27541 tacggatgat ttgcagtttg gcaaatcatc cgctctaaga tgattcctgg ttgataatta
    27601 agactattta cctgttatta acactctcaa gatataaaat tattatcagc gatataacag
    27661 gaagtaatta tcacctgcgt gatataaccc tgcgcgcgag cagatttcac ggaataattt
    27721 caccaggctt attcttagct attatagtta tagagagctt acttccgtga atcataaatt
    27781 caggagagag tattatgttt gtaacgagca aaaaaatgac cgcggctgtt ctggcaatta
    27841 ctttggcaat gtctctgagt gcctgttcta actggtctaa acgggaccgc aacaccgcaa
    27901 tcggcgcggg tgcaggggca ttaggcggtg cagtactgac cgatggcagt acgttgggta
    27961 cattaggtgg tgcagctgtc ggtggtgtta ttggtcacca ggtcggtaaa taatcgtaaa
    28021 taaatgtcga ttatcgactc gcaattataa tacgcttgat tctgttcagt acgaaataac
    28081 tgaataatac acacagtaaa atattcgaaa gccacggata tatccgtggc tttttatgtt
    28141 aaccgcctgc gagttttacc ttcatccctt tcgcttcaag tagtgatttt aataaatcac
    28201 gcttatcgcc ctggatttca ataactccat ctttaactgc tccgccgcag ccgcattttt
    28261 tcttcagttc cgctgcaagt tttgtcagtt cggcatcatc gagatcgata ccggtaatca
    28321 ggcaaacgcc cttaccttta cgtccactgg tctgacgctg aatacgcacc gctccatcgc
    28381 ctttagggcg tactggggcc gctttgggct catcaatacg cccggtttcc gttgagtaga
    28441 ccagacggct gttggaatca ctcatcatgc actccgctgt aaagaggcgt tgatcgcttt
    28501 cagtgtctgc gctggatcta ccgattgcgt taccgggcga ccaatcacca tataatcaac
    28561 accagccgcc aacgcctgtt ctggcgtcat aatgcggcgc tggtcaccag cttcactccc
    28621 ctgcggacga atgcccggtg taaccagttt gaactcctga ccgaatacct gtttaaagcg
    28681 cacagcttcc tgagcagaac acaccacacc atcaaggtca catttttgcg tcagtgctgc
    28741 cagacgttct gcataatctg caggtgacag tgtcacgcca agatcggcca ggtcgctggc
    28801 ttccatgctg gtcaacactg tcacagcaat caaaagcggt gcatctttgc caaacggaac
    28861 cagtgcctca cgcgctgcgg tcatcatacg cgccccaccg gaggcatgaa cattcaccat
    28921 ccacacgcct aagtcagctg cagcagcgac agcgtgcgct gcagtgttgg ggatatcgtg
    28981 gaatttcagg tcaagaaaga tatcaaaacc acgctgttga agttcgcgca caaactgtgg
    29041 cccaaacaat gtaaacatct ctttaccgac cttcagacga caatcgcgtg ggtcgatctt
    29101 gtcgacaaag gacagcgcgt catcacaatt atgataatca agggcaacaa ccacaggaga
    29161 attcgtaacg gcgcgggaag aagatgaagc agttaacgtc atgaccagac cttcttgatg
    29221 atgggcaccg ggagtggcgc gaaacaggta aacggcgagc attctacccg tgaaaataaa
    29281 aaattaacag ttgcgatttt tcccctagca ggctttttct gctccgtcgt tgccggagca
    29341 caatgggaca tcaataacta gtatgttgta actaaagtcg gattttttta aaaattacag
    29401 gccatcaaga ccgcgaatcg gtttaatagt tgaccaggca cgacaagacg gacaatgcca
    29461 gtagagggtg tatgcagtaa aaccacattt ctggcagcta taacgaggct tactacgcac
    29521 cttctcgcca accatgtcac gcagcaccat cagactctct ttggcgcgcc cttcttccgc
    29581 ttcatttaag tggtaatcca ttagcttatg gaatacacgc atggtcggat gacgctgaag
    29641 ctggcgtgta atatagacct gtgcagcctc actaccgtcg cgcgcttcga tgatatcagc
    29701 aagcatcaat tcagcatcgg caccggtgtt ctcttccacc gcgcgttgca ggaattctgc
    29761 ccattcggca gttttaccca actgctgata gcaggtttgc aacatttcca gcgtttcgct
    29821 gaccagttct ctgtcctggg atatgacgcg ttgcaaactt tcgacggctt tggcgtattc
    29881 tcctttcgcc ataaacacgc gtcccatcat gatggatacg cgggcgctgt ttttatctgc
    29941 cgccgcccct ttttttagca aggtcatggc acgatcgaga tcgtcgctgg ccatatgctg
    30001 cagggctaac tcacagtaga aatgggcaat ttcgacgcgc tgtttatctt tacccagctt
    30061 caccaggcgt tcggcaacat caattgcttt ctgccactca ctggtagcct ggtagatttg
    30121 tagcaactgt tgcagcgcgc caatgcggaa gtcagtttca tcggtcagct gattgaacat
    30181 gtcttccgcg cggtcgtata acccggcggc catgtaatca cgccccagtt gttgaatcgc
    30241 caacagacgc tgttcatagg tcagcgaggc gctttccatt agggtctgat ggatgcgaat
    30301 agcgcgatca acttcgccac gcgaacggaa caggtttccg agcgtaaggt gggcttcaac
    30361 ggtgcctgta tcctctttaa gcatatcgag aaacaggtct accgctttat cctgttgatt
    30421 actaagcagg aagttaaccc ccgctacgta atcacgcgac aagcggttgg cttcatcttg
    30481 cttgttttgt tgcgcacttc tgcggcccat ataccagcca taggcagcgg ctacaggcaa
    30541 aagcagaaac aacaactcca gcatagaaag ttattccttc gccgctgacg agtgcggcac
    30601 tacagccacg tcagtcgcgg gtgaaagctg gttttccagt cgctttattt tacgttcagc
    30661 gcgcgccagg gaaacacgaa ctcgcagcca gaacaggcca caaatcaacc aaccgatagc
    30721 aaaccccgca gcaaacaata ccgccagcaa tgtggaaata cggtactccc cttgcgctaa
    30781 cagataatta aacgtcacct gttgatcgtt ctgcgcaccc aacgtgaccg aaatcacgaa
    30841 gatcgctaac accagtaaga aaatgagtaa atatttcaca ttacttcccg ttatgtggtt
    30901 agagcgaata aattatggtc aacttaccgc aatagccctt taaagtacca ttttcgcgcc
    30961 gtctgcgaaa tggcatccac gcgcattgtt gatgatttaa ggcctaaatt cggaaaatca
    31021 accagcgtta actttcttgt tctcgttgtg caatttcgcg attttcttcc gcaggtggtg
    31081 ttaatggccc acaaattcgt tgcgcaagcc acgttgccac cgccaccagc gcccacgaaa
    31141 tcaacgtagc taccaccaga tcgcgtggcc aatgcatccc gagcagcagg cggcttccca
    31201 tgactcccgt tgcccagacc aacaagatag caatggttaa cgttcgccga cgcggccaca
    31261 gcaaaccaac ggccagcagt gcccaactgg cagcaaacat cgtgtgaccg gaaggaaagg
    31321 caaaccccgt ctctttctgc cagtgtgaac gcaaatattg tggaatattt ttctcttcag
    31381 ccaactgttc ttttactaga tttccgcgtt ctgctcgctt taaagtgtag aactcatcaa
    31441 ccggaatatg atgtgttttt tccaaccaga taacaaaagg tcgtggttcc tgtactttgt
    31501 ctttgatcca ggatttaacg ccttgtccaa caaggattgc cgccgccaga atggcaaata
    31561 atacaaaggc agccttaatg cgaaaacgca gacaccagag aaaccagccg aacaaaatca
    31621 aatgtgtaat gacgccccag ggctgggtga cagtttcagt aacccaaaaa gccgctttta
    31681 gtagccaact ttgttctcca ggttgccaac gccagccaga aatccatacg gctactggca
    31741 tgacaagcaa tagtgcagct cccactgcgg tacgtcgggc aatcgaacgc atggcctctc
    31801 ctttttgata agtcccacaa tcataactga aaatgccagt tccaagaaaa attgacagat
    31861 ttgtgccatt ccgtgaacga tcgacgcgtc gtgattaggt gaaccccttc tcgttatggc
    31921 aaaataagcc aatacagaac cagcattatc tggagaattt catgcagctt aaacgtgtgg
    31981 cagaagccaa actgccaacc ccatggggcg atttcctgat ggtgggattt gaagaactgg
    32041 caaccggaca cgatcatgtc gcgctagtct atggcgatat ttccgggcat accccggtac
    32101 ttgcgcgcgt ccattccgaa tgtctgaccg gtgacgccct gttcagcttg cgctgcgatt
    32161 gtggcttcca gctcgaagcg gcattgacgc aaattgccga ggaaggccgt ggtattttgc
    32221 tgtatcaccg tcaggaaggt cgtaacattg gtctgctgaa taaaatccgc gcttacgcac
    32281 tgcaggatca aggttacgat accgtagagg ctaaccacca gttaggcttc gccgctgatg
    32341 agcgcgactt cactctttgc gctgatatgt tcaaactcct tggcgtcaat gaagtccgct
    32401 tgttaaccaa taacccgaaa aaagtcgaaa ttctgaccga agcagggatt aatattgttg
    32461 aacgcgtacc attgattgta ggtcgtaacc ccaataacga acattatctc gataccaaag
    32521 ccgagaaaat gggccatttg ctgaacaaat aaccctcttg catcgtgtaa ttcatttgct
    32581 tgccggaagc aaaataaccg gcaagcaaat acttgttact tcaacatatt acgaatgaca
    32641 taatgcaaaa tgccgtcgtt ctggtagtag gtcaactccg tcgcggtgtc gatacgacaa
    32701 cggcagggta cgacttcctg gctaccatcc gcgcgcgtaa gcgtcaccgg aaccgtcgcg
    32761 ccaggttgta gattttgcag atcgccaata tcaatcttct cttccccggt tagccctaac
    32821 gttttacgcg tcacgccttg cggaaattcc agcggcagga tgcccatgcc aattaaattc
    32881 gaacggtgaa ttcgttcaaa cgattcggca atcaccacac gaattccaag cagacgaggt
    32941 ccttttgccg cccagtcacg gcttgagcct gatccatact ctttcccggc aatcaccgcc
    33001 agcggcgttt gctcctgctt atagcgcatc gcagcatcat aaatagagac tacgtcgctg
    33061 tcaggtaaat gccgcgtcat ccccccttca acgccaggca ccatttcatt acggatgcga
    33121 atattggcga aggtgccgcg catcatcact tcatggttac cacgccgcga accgtaggag
    33181 ttaaagtctt ttcgctcaac accccgacct tgtagatatc gacccgctgg gctgtcgggc
    33241 ttaatactgc ccgccggaga gatatggtca gtggtgactg aatcccccag cattgcgagg
    33301 atccgcgcac cgtgaatatc ttccactggt gctggtgttg cctgcatttc atcaaagaaa
    33361 ggcgataagc gaatataggt tgagtcctcc tgccaaccgt aggtatcgga tcgtgtgacg
    33421 ttaatttcct tccactctgc tgtgccttca aaaacttctg cgtactcttt gcggaacatt
    33481 tctgtggaga cttgttctac cgcacgggca atttcttgtg ccgatggcca gatatctttc
    33541 agataaaccg gctcgccttt gcgatcatgg ccgataggct cagaagccag gttgatattc
    33601 atatttcccg ccagcgcata ggcaaccacc agcggtggcg aggccagcca gttagtttta
    33661 accagcggat ggatacggcc ttcaaagtta cggttgccgg acagcaccgc accgacggtt
    33721 aaatcgcctt ttttgattgc cgtttcgata ggatcgggca gcggcccaga gttaccaata
    33781 caggtggtac aaccgtatcc cacaaggtta aaccccagtt cgtcgagata cggtgtcagt
    33841 ttcgcttttg ccagataatc agaaacgact ttcgaacccg gtgccaacga cgctttgacc
    33901 cacggttgcc gcttgaggcc aagagttacg gcttttttcg ccagcaagcc tgcggccatc
    33961 agcacacttg ggttagaggt gttggtgcac gaggttatcg cagcaatgac cacagcgcca
    34021 tcaggtaact gatactgatg tccgttcata acataatcga ctggcaggcg atctttatgc
    34081 gtggcattca cttccagttc gttactggcg gcaaatgctt ttggtacatc gggcagtgca
    34141 acgcgatcct gtgggcgttt tggccctgcc aggctcgctt caacgtcatt catatccagt
    34201 tctaacatac tggtaaaaat cggttcatcg cccgggttac gccacatgcc ctgcgctttg
    34261 gcatattttt cgaccaactc gacctgatct tcgctgcgcc cgcttaaacg catgtaatcg
    34321 agggttactg catcgattgg gaagaagcca caggtggcac catattctgg cgacatattg
    34381 gcaatggtgg cgcgatccgc caacggtagt gaatccagac catcaccata aaattcgacg
    34441 aatttcccca ccacgccatg tttgcgcagc atttgggtaa cagtgagaac caggtctgtg
    34501 gcggtaatac cttcacgtaa ttttccggta agtttaaagc ccactacgtc cgggataagc
    34561 atggaaaccg gctggcctaa catggcggct tctgcttcga tcccacccac gccccacccc
    34621 agcacgccaa ggccgttgat catagtggtg tgcgagtcag taccaacgag tgtatccgga
    34681 taagcaatcc attcaccgtc ctgcaattcg ctccacactg ctttgccgag atattcgagg
    34741 ttaacctgat ggcaaatgcc tgtgcctggc ggcacgacgc taaaccgact gaacgcttgc
    34801 tttccccatt tcaggaacac ataacgttcg tggttgcgct ccatttccag gcgtacgttt
    34861 tcttcaaatg cctcatcatc accaaaacga tcgacggtca ccgagtggtc aatgaccagg
    34921 tcgaccggtg agagcgggtt aacctttgca gtatcgccac cgaggcgttt aaccgcttcg
    34981 cgcattgccg ccagatcaac aacggcaggt acgccggtaa agtcctgcat cagcaccctt
    35041 gccgggcggt aggcaatttc acggtcagca tgggcatttt tcagccatcc cgccagtgcg
    35101 tggatatcct cttcggtaac tgagttacca tcctgccagc gcagcaggtt ttcgagcaaa
    35161 actttgagtg acttgggtag acgggtgata tcgcccagtg atttagcagc aagcggcagg
    35221 ctgtagtagt ggtaagtttt atctttggcc tgcaacgtgt ccttactggc ttctcgtagg
    35281 gttgacgaca tagctcctcc ttaatgacag ggttgcgaag ccctgattct cttcagggtg
    35341 aatattaaag ataacacaaa catcgcgtaa cgatttgata acaacccaaa ttgataaaag
    35401 aggagaaacc ttgtggcaaa gcaaatgaca accccgccgc agcggggtca aggatcagtg
    35461 agtaatgatc cagaggagtt cggcccagaa gagcatcgac agagaaaacg cgcccatcca
    35521 ggaccaatat ttcatatttg tgttgatcat aggctacacc cttaacggtt attattttgt
    35581 gttgagataa ctcaagctgt tattgctgcg gtgtattaaa agccctttaa ttcattaaaa
    35641 atgggtaata agaatgatga taattattgt ttcggctgat catcttcagc aaacagttct
    35701 ataaaggctt gctgctgcga tgttagttgc cagtcgtctg gaataacgac ggaacattcc
    35761 cgtttcgttt ctggttcatt gtcactatgc tggcataatg gtgacgactc cagttgctcc
    35821 tgaccgacca tagttatccc cacactttcc aaatcagcag tgcgacaacc acccagaaaa
    35881 gtgcagaacc aagaaaaacc gccagccagg ctttacgttt taattcagga tcacggcggg
    35941 gttcctgatt tccagaaggc attgctaacc tcatataatc gccatcactt atcagcaaga
    36001 cgttgaatga ttggtgtaat taatcatacg atgagacaaa tcctaaagaa actttagtca
    36061 aaaatccagc gaatttacga atcgtctcca gtgaataatt caatcttttg acctgaaata
    36121 gatatttgaa aactggaatt tgcgaggcgg gtaattagac actactttac aggtaattta
    36181 ccctataaat atgagggata aaaatattgt gcacggtata gaaataagta acaaaagaga
    36241 atatattccg gcaccttcgc tacataaaag gtgccgaaaa taacgcaaga aattattttt
    36301 ccggcagttt tatatcttta aacatgacct caatttcttc attggagcgc aatgcgacag
    36361 ccgcatcaac gacatcacgc gttaaatgcg gtgcaaaacg ctgaatgaaa tcatacatat
    36421 aactgcgcaa aaaagtacta cggcgaaaac caattttggt tgtactgtgg ctgaagatat
    36481 cgtgagcatc aacgcgcaca aggtcgggat cggcgaccgg atccaccgcc atgctggcaa
    36541 tgacccctac ccccagccct aaccggacgt aagttttaat gacgtcagca tccgttgccg
    36601 tgaaaacgat acgcggcgtt aaccctgcgc ggttaaaggc agtatccagt tctgaacgtc
    36661 cggtaaagcc gaaggtatat gtcaccaacg gatattgcgc cagttcttca atggtaatgg
    36721 cttttttgcc tgccagcggg tgatccggag tgactacaat agcccgattc cagtggtagc
    36781 acggtaacat cactaaatct tcatacagat gcagcgcttc tgtggcgata gcgaaatcgg
    36841 cattgccttt agagacggca tcagcaattt gtgtcggcgg gccctggtgc atatgcaaag
    36901 aaacgcgggg ataacgctca ataaagcctt tgataacgtt tggtaacgcg tagcgtgcct
    36961 gggtatgcgt ggtggcgata tacagcgagc ctttatccgg ccaggtgtgc tcaccggcaa
    37021 ccgattttat ggcatcgact ttcgacagga cttcgcgagc gatacgaatt atttcttgtc
    37081 ctgctggcgt tacctgcgtc aggtgcttgc cgcttcggga aaaaatttga atgcctagct
    37141 cgtcttccag cattctgact tgtttactga tcccgggttg tgatgtgtaa agtccttccg
    37201 ctgttgatga gacattcagg ttatgattga ccacctcaac aatatagcga agttgttgta
    37261 atttcatgtt aaaccatcca cttagacgcc atcagaacat cgttttttcg tcacacctgg
    37321 cgggtgacta atacgattta ataataaaaa aggtgctaac tataaccact atatcattta
    37381 tagccttagt gtataggtgt gaagaaaaaa taatatgcaa taaaaaaggg ccgctttcgc
    37441 gaccctttgt ttataaaaac ctgacagaat taaaggttat ttttttcctt caacccattt
    37501 gccatcaaca taaaatgctg accagccagt cgcctttccg tctttttccg aagagacata
    37561 ttgctgtttg gttttacggc taaagcgaac catggtctta ttaccttccg gatcctgctg
    37621 tggcgcatcg gccagataac gcagtttttc cggcagacgg tcgcggaagc gataaagctc
    37681 ttccaccagt ggcgcacgcg tttcacgcga tttcgggaaa gtgttggcag ccaggaacac
    37741 accggcagca ccgtcacgca gcacgaaata agcatctgat ttttcgcacg gcagctcagg
    37801 taatggcacc ggatcttctt tcggtggtgc cacttcgccg ttacgtaaaa tcttacgtgt
    37861 gtttttacac tcttcgttgg tgcaggccat gtatttaccg aaacgcccca ttttcaggtg
    37921 catttcagag ccacattttt cacactcaac gatcgggccg tcataacctt taatgcggaa
    37981 ttcgccctct tcgatctcgt aaccgtcgca ggttgggtta ttaccacaga catgcaactt
    38041 acgtttcgga tcgatgagat agctgtccat cgccgtgccg catttcgggc aacgacgttt
    38101 tgcgcgcagc gcgttggttt cagcatcttc gccttccagc acgttcagca cttcgttttc
    38161 cggcaccagg ttaatggtgg ttttgcaacg ctctttcggc ggcagcgcat agccagaaca
    38221 gccaaggaat accccggtac tcgctgtgcg aatccccatt ttgcgaccac aagtcgggca
    38281 gtcaatgctg gtcagaacca tctggttcgg gcgcatacca ccctcttccg gatctttttc
    38341 agctttatct aactgctggg tgaaatccga gaagaagtga tccagtacag ctttccactc
    38401 tgcttcgtga tttgccacct ggtcgaggct gttttccatc tgcgcggtaa agtcgtagtt
    38461 cattaactcg cggaaatttt cttcaaggcg atcggtgacg atttcgccca ttttttccgc
    38521 atagaaacga cgattttcta ctcgcacata gccacgatcc tgaatggtcg aaatgatcga
    38581 cgcataggta gacggacgac cgataccacg tttttccagt tctttaacca gcgatgcttc
    38641 actgaaacgg gctggcggct tggtaaagtg ctgggctggt gttagttcaa cgagcgtcag
    38701 agcatcgcct ttatcgactg ccggtaagat acgatcttca tcgcctttac gcagtgcagg
    38761 catcactttt gtccagccat caaagcacaa aatacgaccg cgtgctttca ggcggaaatc
    38821 gcccgcacca acggtcagcg tcgtggagtc atatttcgct ggggtcatct ggcaggcaac
    38881 gaactgacgc cagattaact ggtacagttt ctgcgcatct gcttccatat ccttcagcga
    38941 ttccgccatc acattgacgt cagaaggacg aatcgcttcg tgcgcttcct gtgagttttc
    39001 tttgctggcg tactgattcg gactttccgg cagatatttc ttaccaaaat tatcgctgat
    39061 ataaccgcga accatattta ccgcgtcctg actcaggtta gtggagtcgg tacgcatgta
    39121 agtgatatag cctgcttcat acaaacgctg cgccatcatc atggtttttt tcacgccaaa
    39181 accaagacgg gtgctggcag cttgttgcag cgtagaggta ataaaaggag cgccaggttt
    39241 actggttgtc ggtttgtctt cacgttccag cacgctgtag cgcgcttttt ccagcagact
    39301 taccgcagcc tgagtttgtt ctttgttgac cggacggaac ggtttgtcat tctgatgagt
    39361 cacctgcaac gccaacgctt caccagatgg cgtagtcgtg ctggcatcga cttcccagaa
    39421 ctcttccggt acgaacgctt taatttcacg ctcacgctct accaccaggc ggaccgccac
    39481 cgactgcaca cgaccggcag acagaccacg agcgatcttt ttccacagca gcggcgaaac
    39541 catatacccc accacgcggt ccataaagcg acgcgcctgc tgggcattaa cacgatcaat
    39601 attcagctca cccggtttgt taaatgcctg gcggatcgcg tttttagtaa tttcgttaaa
    39661 caccactcgg ctatagcgcg catcatcacc accaatcact tcccgcaggt gccatgcaat
    39721 ggcttcccct tcgcggtcaa ggtcggttgc gagatagatg tggtcggctt tttcagccag
    39781 ttgtttcagt tcagagacga ccttctcttt accaggcaac acttcatagt gcgcctccca
    39841 attgtgccac gggtcaaccc ccatacggtt gacgagagcg ccacgttcat cctttttagg
    39901 ctttttagcc gtcttggtgg aggtagagtc ggcactcttt ttggcagctg agccactggt
    39961 cggcaaatcg cggatgtgac cgacgctgga tttcaccacg tagtcacaac ccagatactt
    40021 gttgatcgtt ttggcttttg ccggggactc aacgatgaca agagctttac ccatattcac
    40081 cttacctaat ttgattcttc caggaatgcg tcgcacgttg ataaaccttc cactggcaac
    40141 gagttaccga tatggactcg ctctctgcgg atatcaaccc cttgtcgtga ctctgttcac
    40201 gaaaattcag gtgactgagt taacaggtct tggcctacag gcgctatagc acgacggaat
    40261 tttggtcgaa tgtcaagcaa atctattgcc agattgacga aagcgtcaca ctgtacctga
    40321 taaaatccga tacgcaactt tattagcatg caaaatccaa agttgccagt aaggcgctct
    40381 tgccaccagc gtggtatatt tgtctgcaag ccatgcctgg cgtagactaa tgtccttgtt
    40441 taaggagtaa ataatgaaca aagagacaca acccatcgac cgcgaaacgc tgttgaaaga
    40501 ggccaacaaa atcattcgtg agcatgagga tacgctcgca gggattgaag ccaccggcgt
    40561 cacgcagcgt aatggcgtgc tggtatttac cggcgactac tttttagatg aacaaggatt
    40621 gccaacagcc aaaagcaccg cggtgtttaa tatgttcaaa cacctggccc atgtgctttc
    40681 agaaaaatat catctggttg attaaagcaa aacgcgaggt cttagcctcg cgtttgtctt
    40741 ttacatcaat ggcttttgac cccgctgcca ccagcgtagc aacaatcgat cggcgctctc
    40801 tgccgcgctg ccggtgaatc ggtcaatgag tcgtttacgc tgcatatagc gtacattaac
    40861 cacttcacgg ccttccatca ggctaagaat aacttcatca ctggtgttga tttcatcaac
    40921 caggcctttc tctaccgcct gttgtccgta ccagtgttca cccgttgcca cctgttcaat
    40981 atccagagac ggacgcatac gcttcacaaa atctttaaat aactgatgcg tttcgttcag
    41041 ctcttcgcgg aatttctccc gcccttcttc ggtattttca cccagcaagg tcagcgtgcg
    41101 cttatactgc ccggcggtgt gcagttcgat atcaatatct ttgcttttca ggaagcggtt
    41161 aaagttgggc atttgcgcca ccaccccaat ggaacccaca atagcaaacg gtgcggaaac
    41221 aattttgtcc gccacacagg ccatcatgta accgccgctg gcagcgactt tgtctaccgt
    41281 aacagttaaa ggaatgtttt tatcacgcag acgctgcagc tgcgaagccg ccaacccgta
    41341 accatgcacc atgccaccag ggctttccag acggagcaca acctgatcct gcggtttgaa
    41401 tgctgcgagt accgccgtta tctcttcacg tagcgagttc acttcatggg cgtccatgct
    41461 gcctttaaaa tccagcaccc agacgcgagg tttactgtca gttgccacct cgcccagttt
    41521 ggctttcgct tttgctgctt tcgcttcttg cttgtgcttt ttcttctgcg ctttgtgcca
    41581 ctgtttttgc tgatgtgagt ccatcagcgc cgcggccagt tcttctttca tctccttata
    41641 ctgttcgctg agattgttga cccgtaactc gccacgctgg cgtttattac gttgagcaac
    41701 attgacaata atggcggcaa tcgccgcaat cgctagcaca acggtaacga ttttagccaa
    41761 aaacaaacca tattcagaca acaattccac gagtccccac cttggttaaa caacgctttg
    41821 gctcacagtt tacaacaggc atgttaagcc gtctcgcaga gatgttattg cagcgaaagt
    41881 atgacaaagt atccattttg cggtgttatc gccccaggct gattgaaatg cccttctgtt
    41941 tcaggcataa agccccaaag tcataaagta cactggcagc gcgacagaca acgtcgttga
    42001 ggagtagcca tgcattacca gccaaaacaa gatttactca atgatcgcat tatcctggtg
    42061 acgggagcca gcgatgggat tggtcgtgaa gccgcgatga cgtatgcacg ctatggtgcg
    42121 acagtgattc tgttgggccg taatgaagaa aaattacgtc aggtagccag ccacataaac
    42181 gaagaaactg ggcgtcagcc acagtggttt attctcgatt tgctgacctg cacgtccgaa
    42241 aattgccaac aactggcaca gcgcattgtc gttaattatc cgcgtctgga tggtgttttg
    42301 cataatgccg gattgctcgg cgatgtttgc ccaatgagcg aacaaaatcc gcaggtctgg
    42361 caggacgtca tgcaggtcaa cgttaatgcc acctttatgc tcacccaggc actgcttcct
    42421 ttattactca aatcggacgc cggctcactg gtctttactt catcaagcgt tggacgtcag
    42481 ggacgagcca actggggtgc atatgcagcg tcgaaatttg ccaccgaagg gatgatgcag
    42541 gtactggccg atgaatatca gcagcgcctg cgcgtcaact gcattaaccc aggcggtacg
    42601 cgcaccgcaa tgcgcgcaag cgccttcccg accgaagatc cacagaaact taaaacaccc
    42661 gctgatatca tgccgctcta cctctggctg atgggcgatg acagccgccg taaaaccggc
    42721 atgacctttg acgcccaacc gggccgtaaa ccaggaattt cccaatgagt gatgaacgct
    42781 accaacagcg tcagcagcga gtgaaagaaa aagtagatgc tcgtgtggcc caggcccagg
    42841 atgaacgcgg tattatcatc gtctttaccg gcaatggaaa aggcaaaacc accgcggcat
    42901 ttggtacggc aacacgcgca gttggtcacg gaaaaaaagt gggcgtcgtg cagtttatta
    42961 aaggcacctg gcctaatggc gaacgcaatc tgctggagcc acatggcgtt gagtttcagg
    43021 tgatggcaac gggctttacc tgggatacac aaaaccgcga gtctgatacc gccgcctgcc
    43081 gcgaagtctg gcaacatgca aagcggatgc ttgctgattc ctcactggat atggttttgc
    43141 ttgatgaact gacgtatatg gtggcgtatg actatttgcc actggaagaa gtggtgcagg
    43201 cgttaaatga acgtccacat caacagacgg tgattatcac gggtcgaggc tgtcatcggg
    43261 atattcttga actggcggat acggtaagtg agctgcgccc catcagacat gcgtttgatg
    43321 ccggtgttaa ggcgcaaata gggattgatt attaatctgt aaaaatgccg gatatgacgt
    43381 ccggcatact ttgattaacc gttattacgc ccgccagaac gacgactgcc gctcacctga
    43441 ctgtgacgtt tcaccgcacg acgaatctga ttagccttca tgcgacgacg gtctttttct
    43501 acagcgactt tagagctggt ttctggcgga agctccacca gttcgcgcag atagttagtc
    43561 tgggcgaggt ccagctccgt ccagccaccg cgtggcagac cttttggcag tgggatatca
    43621 ccgtaacgaa cacggatcag gcggctcacc tgcacaccaa ccgcttccca cagacgacga
    43681 acttcacggt tacggccttc ggtcagagtc acgttgtacc actggttgat cccttcgccg
    43741 ccgctgaact tgatggtttt aaaagctgcc ggaccatctt ccagttgcac gccacgactc
    43801 aaatcacgca gtttcgcgtc gtcaacctga ccaaatacac gcacggcgta ttcacgttca
    43861 acttcacggc ttggatgcat taaacggttt gccagttcac catcggtggt gaacagcagc
    43921 agaccacagg tgttaacgtc cagacgacct acggcaatcc agcgtgcgcc acgcagcttt
    43981 ggcagacggt caaacaccgt cggacgccct tccggatcgt tacgggtaca caactcacct
    44041 tccggtttgt aataggccag aacgcgacaa atttgttcag cggactcacg taccgaaatc
    44101 agatgaccat cgatacgaat tttcagccca ggggtaactt caacgcgatc gccgagttta
    44161 gcaattttgc catcaacact cacacgaccg gcttcaataa tagattcgat ttcacggcgg
    44221 gagccgtggc cagcgcgggc cagcactttc tgtaattttt cgctcataga gcttcctttt
    44281 ggtgtcgcct tcacaggcgt cgaacaggag aatcaatggc gccgtatagc gccattttaa
    44341 ggccgcgtag tatacagagt tacgcactta taagaaaggc ttcacatcac ctacgccttc
    44401 acgcaccacg accggcgtat catcggtaag atcaataacc gttgttggtt tctgcccgag
    44461 atagccgccg tgaataatca aatctacctg tttttccaga cggtctttaa tttcttcggg
    44521 atcggattcg gtaaattcgc tgcctggcag cattagcgaa gtggaaagca tcggttcgcc
    44581 cagtgcttca agcaacgcct gcgcaatcgg gttagaaggc acacgcatac cgatggtttt
    44641 gcgtttctcc tgcaacagac ggcgtggcac ttccttcgtc cccttcagga tgaaggtata
    44701 gttgcccggc gtgttgtttt tcatcaaacg aaacgcaacg ttatccacga atgaataggt
    44761 cgagagttca gaaagatcgc gacacatcag ggtaaagttg tgaccgtccg gcagctggcg
    44821 aatacgacaa atacgctcca tggcgttttt atcttcaatt ttacagccga gcgcatagcc
    44881 ggaatcagtt ggatagacaa tcaccccgcc tttacgcacg atctccaccg cctggttgat
    44941 cagacgttgc tgcgggttat caggatgaat ataaaaaaac tggctcataa ttccctctct
    45001 gtggtgttct gcggctgttc ccatagctgc cagacgcctt caacgcctgc gggcagccag
    45061 agtttacgac ccagttcgat ccacgggcat ggctgatgaa aatcagatcc ttgtgatgcc
    45121 cataaatgat gctgacgcgc aagtgtggcc agctgggtgc gttcattggg cgattgctgg
    45181 cactgcgcga cttccatcgc gtcgccgtgg tgttcggcaa aatgcgcaac caatcttttc
    45241 agccatttag cactaagatt gtaccgccca ggatgagcta gcaccgcctt accgccagaa
    45301 tgatgaatga catcaatagc ttgttctatt gtacaccact gtggcggaac gtatccggtt
    45361 ttcccgcgcg ccagatactt tttaaagaca tctgccattg aactggcttt gccgcactcc
    45421 acaaggaaac gcgcaaaatg accgcgcgtc actgcgccgc cctgcgccag tcgttgtgcg
    45481 ccttccagcg ccccaggtat ttgcgctttt tcaagtcttt cggcaatttg ctgcgcccgc
    45541 tgattgcgcc gttctgtctg ctgcgcgagg aactcacaca ttagcggatg agtaatatca
    45601 atattcagcc cgacaatatg aatttcatga ttttcccaga ccgtggaaat ttccacgccg
    45661 ggaataagat tcagcgccag tcccgaacgt gaaatttcct ctcttgctgg cgcgattgca
    45721 gctgtggtgt catggtcggt gatcgccagg gtgccgacgc gcatctcgac tgcacggtgc
    45781 accaatgctt ctggcgtcag gcagccatcg gaagctgtgg tatggctgtg caggtcgtaa
    45841 atcactgcat aattcgtgtc gctcaaggcg cactcccgtt ctggataatg ttttttgcgc
    45901 cgacatcata acgggtctgg caaatattct gaaatgagct gttgacaatt aatcatcgaa
    45961 ccagttaact agtgcgcaag ttcacgtaaa aagggtatcg acaatgaaag caattttcgt
    46021 actgaaaggt tggtggcgca cttcctgaaa cgggcagtgt attcaccatg cgtaaagcaa
    46081 tcagataccc agcccgccta atgagcgggc ttttttttga acaaaattag agaataacaa
    46141 tgcaaacaca aaaaccgact ctcgaactgc taacctgcga aggcgcttat cgcgacaacc
    46201 cgactgcgct ttttcaccag ttgtgtggga atcgtccggc aacgctgctg ctggaatccg
    46261 cagatatcga cagcaaagat gatttaaaaa gcctgctgct gatagacagt gcgctgcgca
    46321 ttacagcatt aggtgacacc gtcacaattc aggcactttc cggcaatggc gaagccctgc
    46381 tggcactact ggataacgcc ctgcctgcgg gtgtggaaag tgaacaatca ccaaactgcc
    46441 gtgtgctgcg cttcccccct gtcagtccac tgctggatga agacgcccgc ttatgctccc
    46501 tttcggtttt tgacgctttc cgtttattgc agaatctgtt gaatgtaccg aaggaagaac
    46561 gagaagcaat gttcttcggc ggcctgttct cttatgacct tgtggcggga tttgaagatt
    46621 taccgcaact gtcagcggaa aataactgcc ctgatttctg tttttatctc gctgaaacgc
    46681 tgatggtgat tgaccatcag aaaaaaagca cccgtattca ggccagcctg tttgctccga
    46741 atgaagaaga aaaacaacgt ctcactgctc gcctgaacga actacgtcag caactgaccg
    46801 aagccgcgcc gccgctgcca gtggtttccg tgccgcatat gcgttgtgaa tgtaatcaga
    46861 gcgatgaaga gttcggtggc gtagtgcgtt tgttgcaaaa agcgattcgc gctggagaaa
    46921 ttttccaggt ggtgccatct cgccgtttct ctctgccctg cccgtcaccg ctggcggcct
    46981 attacgtgct gaaaaagagt aatcccagcc cgtacatgtt ttttatgcag gataatgatt
    47041 tcaccctatt tggcgcgtcg ccggaaagct cgctcaagta tgatgccacc agccgccaga
    47101 ttgagatcta cccgattgcc ggaacacgcc cacgcggtcg tcgcgccgat ggttcactgg
    47161 acagagatct cgacagccgt attgaactgg aaatgcgtac tgaccataaa gagctgtctg
    47221 aacatctgat gctggttgat ctcgcccgta atgatctggc acgcatttgc acccctggca
    47281 gccgctacat cgccgatctc accaaagttg accgttattc ctatgtgatg cacctcgtct
    47341 ctcgcgtagt cggcgaactg cgtcacgatc ttgacgccct gcacgcttat cgcgcctgta
    47401 tgaatatggg gacgttaagc ggtgcgccga aagtacgcgc tatgcagtta attgccgagg
    47461 cggaaggtcg tcgccgcggc agctacggcg gcgcggtagg ttatttcacc gcgcatggcg
    47521 atctcgacac ctgcattgtg atccgctcgg cgctggtgga aaacggtatc gccaccgtgc
    47581 aagcgggtgc tggtgtagtc cttgattctg ttccgcagtc ggaagccgac gaaacccgta
    47641 acaaagcccg cgctgtactg cgcgctattg ccaccgcgca tcatgcacag gagactttct
    47701 gatggctgac attctgctgc tcgataatat cgactctttt acgtacaacc tggcagatca
    47761 gttgcgcagc aatggtcata acgtggtgat ttaccgcaac catattccgg cgcagacctt
    47821 aattgaacgc ctggcgacga tgagcaatcc ggtactgatg ctttctcctg gccccggtgt
    47881 gccgagcgaa gccggttgta tgccggaact cctcacccgc ttgcgtggca agctgcccat
    47941 tattggcatt tgcctcggac atcaggcaat tgtcgaagct tacgggggct atgtcggtca
    48001 ggcgggcgaa attcttcacg gtaaagcgtc gagcattgaa catgacggtc aggcgatgtt
    48061 tgccggatta acaaacccgc tgccggtggc gcgttatcac tcgctggttg gcagtaacat
    48121 tccggccggt ttaaccatca acgcccattt taatggcatg gtgatggcag tacgtcacga
    48181 tgcggatcgc gtttgtggat tccagttcca tccggaatcc attctcacca cccagggcgc
    48241 tcgcctgctg gaacaaacgc tggcctgggc gcagcagaaa ctagagccag ccaacacgct
    48301 gcaaccgatt ctggaaaaac tgtatcaggc gcagacgctt agccaacaag aaagccacca
    48361 gctgttttca gcggtggtgc gtggcgagct gaagccggaa caactggcgg cggcgctggt
    48421 gagcatgaaa attcgcggtg agcacccgaa cgagatcgcc ggggcagcaa ccgcgctact
    48481 ggaaaacgca gcgccgttcc cgcgcccgga ttatctgttt gctgatatcg tcggtactgg
    48541 cggtgacggc agcaacagta tcaatatttc taccgccagt gcgtttgtcg ccgcggcctg
    48601 tgggctgaaa gtggcgaaac acggtaaccg cagcgtctcc agtaaatccg gctcgtcgga
    48661 tctgctggcg gcattcggta ttaatcttga tatgaacgcc aataaatcgc gccaggcgct
    48721 ggacgagtta ggcgtatgtt tcctctttgc gccgaaatat cacaccggat tccgccacgc
    48781 gatgccggtt cgccagcaac tgaaaacccg caccctgttc aatgtgctgg ggccattgat
    48841 taacccggcg catccgccgc tggcgttaat tggtgtttat agtccggaac tggtgctgcc
    48901 gattgccgaa accttgcgcg tgctggggta tcaacgcgcg gcggtggtgc acagcggcgg
    48961 gatggatgaa gtttcattac acgcgccgac aatcgttgcc gagctgcatg acggcaaaat
    49021 taagagctat caattgaccg ctgaagattt tggcctgact ccctaccacc aggagcaact
    49081 ggcaggcgga acacctgaag aaaaccgtga cattttaaca cgcttgttac aaggtaaagg
    49141 cgacgccgcc catgaagcag ccgtcgctgc gaacgtcgcc atgttaatgc gcctgcatgg
    49201 ccatgaagat ctgcaagcca atgcgcaaac cgttcttgag gtactgcgca gtggttccgc
    49261 ttacgacaga gttaccgcgc tggcggcacg agggtaaatg atgcaaaccg ttttagcgaa
    49321 aatcgtcgca gacaaggcga tttgggtaga aacccgcaaa cagcagcaac cgctggccag
    49381 ttttcagaat gaggttcagc cgagcacacg acatttttat gatgcgctac agggtgcacg
    49441 cacggcgttt attctggagt gtaaaaaagc gtcgccgtca aaaggcgtga tccgtgatga
    49501 tttcgatccg gcacgcattg ccgccattta taaacattac gcttcggcaa tttcggtgct
    49561 gactgatgag aaatattttc aggggagctt tgatttcctc ctcatcgtca gccaaatcgc
    49621 cccgcagccg attttatgta aagacttcat tatcgaccct taccagatct atctggcgcg
    49681 ctattaccag gccgatgcct gcttattaat gctttcagta ctggatgacg accaatatcg
    49741 ccagcttgca gccgtcgctc acagtctgga gatgggggtg ctgaccgaag tcagtaatga
    49801 agaggaactg gagcgcgcca ttgcattagg ggcaaaggtc gttggcatca acaaccgcga
    49861 tctgcgtgat ttgtcgattg atctcaaccg tacccgcgag cttgcgccga aactggggca
    49921 caacgtaacg gtaatcagcg aatccggcat caatacttac gctcaggtgc gcgagttaag
    49981 ccacttcgct aacggttttc tgattggttc ggcgttgatg gcccatgacg atttgcacgc
    50041 cgccgtgcgc cgggtgttgc tgggtgagaa taaagtatgt ggcctgacgc gtgggcaaga
    50101 tgctaaagca gcttatgacg cgggcgcgat ttacggtggg ttgatttttg ttgcgacatc
    50161 accgcgttgc gtcaacgttg aacaggcgca ggaagtgatg gctgcggcac cgttgcagta
    50221 tgttggcgtg ttccgcaatc acgatattgc cgatgtggtg gacaaagcta aggtgttatc
    50281 gctggcggca gtgcaactgc atggtaatga agatcagctg tatatcgata cgctgcgtga
    50341 agctctgcca gcacatgttg ccatctggaa agcattaagc gtcggtgaaa ccctgcccgc
    50401 ccgcgagttt cagcacgttg ataaatatgt tttagacaac ggccagggtg gaagcgggca
    50461 acgttttgac tggtcactat taaatggtca atcgcttggc aacgttctgc tggcgggggg
    50521 cttaggcgca gataactgcg tggaagcggc acaaaccggc tgcgccggac ttgattttaa
    50581 ttctgctgta gagtcgcaac cgggcatcaa agacgcacgt cttttggcct cggttttcca
    50641 gacgctgcgc gcatattaag gaaaggaaca atgacaacat tacttaaccc ctattttggt
    50701 gagtttggcg gcatgtacgt gccacaaatc ctgatgcctg ctctgcgcca gctggaagaa
    50761 gcttttgtca gcgcgcaaaa agatcctgaa tttcaagccc agttcaacga cctgctgaaa
    50821 aactatgccg ggcgtccaac cgcactgacc aaatgccaga acattacagc cgggacgaac
    50881 accacgctgt atctcaagcg tgaagatttg ctgcacggcg gcgcacataa aaccaaccag
    50941 gtgctggggc aggcgttgct ggcgaagcgg atgggtaaaa ccgaaatcat cgccgaaacc
    51001 ggggccggtc agcatggcgt ggcgtcggcc cttgccagcg ccctgctcgg cctgaaatgc
    51061 cgtatttata tgggtgccaa agacgttgaa cgccagtcgc ctaacgtttt tcgtatgcgc
    51121 ttaatgggtg cggaagtgat cccggtacat agcggttccg ccacgctgaa agatgcctgt
    51181 aatgaggcgc tacgcgactg gtccggcagt tatgaaaccg cgcactatat gctgggcacc
    51241 gcggctggcc cgcatcctta tccgaccatt gtccgtgagt ttcagcggat gattggcgaa
    51301 gaaaccaaag cgcagattct ggaaagagaa ggtcgcctgc cggatgccgt tatcgcctgt
    51361 gttggcggcg gttcgaatgc catcggcatg tttgctgatt tcatcaatga aaccaacgtc
    51421 ggcctgattg gtgtggagcc tggtggtcac ggtatcgaaa ctggcgagca cggcgcaccg
    51481 ctaaaacatg gtcgcgtggg catttatttc ggtatgaaag cgccgatgat gcaaaccgaa
    51541 gacgggcaga ttgaagaatc ttactccatc tccgccggac tggatttccc gtctgtcggt
    51601 ccacaacacg cgtatcttaa cagcactgga cgcgctgatt acgtgtctat taccgatgat
    51661 gaagcccttg aagccttcaa aacgctgtgc ctgcacgaag ggatcatccc ggcgctggaa
    51721 tcctcccacg ccctggccca tgcgctgaaa atgatgcgcg aaaacccgga taaagagcag
    51781 ctactggtgg ttaacctttc cggtcgcggc gataaagaca tcttcaccgt tcacgatatt
    51841 ttgaaagcac gaggggaaat ctgatggaac gctacgaatc tctgtttgcc cagttgaagg
    51901 agcgcaaaga aggcgcattc gttcctttcg tcacgctcgg tgatccgggc attgagcagt
    51961 cattgaaaat tatcgatacg ctaattgaag ccggtgctga cgcgctggag ttaggtatcc
    52021 ccttctccga cccactggcg gatggcccga ccattcaaaa cgccactctg cgcgcctttg
    52081 cggcaggtgt gactccggca caatgttttg aaatgctggc actgattcgc cagaaacacc
    52141 cgaccattcc cattggcctg ttgatgtatg ccaatctggt gtttaacaaa ggcattgatg
    52201 agttttatgc ccagtgcgag aaagtcggcg tcgattcggt gctggttgcc gatgtgccag
    52261 ttgaagagtc cgcgcccttc cgccaggccg cgttgcgcca taatgtcgca cctatcttca
    52321 tctgcccgcc aaatgccgat gacgacctgc tgcgccagat agcctcttac ggtcgtggtt
    52381 acacatattt gctgtcacga gcgggcgtga ccggcgcaga aaaccgcgcc gcgttacccc
    52441 tcaatcatct ggttgcgaag ctgaaagagt acaacgctgc acctccattg caggggtttg
    52501 gtatttccgc cccggatcag gtaaaagcag cgattgatgc aggagctgcg ggcgcgattt
    52561 ctggttcggc cattgttaaa atcatcgagc aacatattaa tgagccagag aaaatgctgg
    52621 cggcactgaa agtttttgta caaccgatga aagcggcgac gcgcagttaa tcccacagcc
    52681 gccagttccg ctggcggcat tttaactttc tttaatgaag ccggaaaaat cctaaattca
    52741 tttaatattt atctttttac cgtttcgctt accccggtcg aacgtcaact tacgtcattt
    52801 ttccgcccaa cagtaatata atcaaacaaa ttaatcccgc aacataacac cagtaaaatc
    52861 aataattttc tctaagtcac atattcctca ggtaattgtt aatatatcca gaatgttcct
    52921 caaaatatat tttccctcta tcttctcgtt gcgcttaatt tgactaattc tcattagcga
    52981 ctaattttaa tgagtgtcga cacacaacac tcatattaat gaaacaatgc aacgcaacgg
    53041 gagaaataac atggccgaac atcgtggtag ttcaggaaat ttcgccgaag accgtgagaa
    53101 ggcatccgac gcaggccgta aaggcggtca gcatagcggc ggtaatttta aaaatgatcc
    53161 gcaacgcgca tctgaagcgg gtaaaaaagg cggtcaacaa agcggtggta ataaatcagg
    53221 caaatcctga ttcaccgtta attattaata tcagctgata aagataattg catctgcggg
    53281 ccgtatggct cgcagtgatt tcactggaga aaaatatgaa tatgaagacc attgaagatg
    53341 tatttattca actgctttca gatacctaca gcgcagaaaa acaattaacc cgggcactgg
    53401 caaaactcgc aagagcaaca tcaaatgaaa aattaagtca ggcttttcat gcgcacctcg
    53461 aggaaactca tggacagatt gaacgaattg atcaagttgt ggagtcagaa tcaaatctga
    53521 aaattaagcg catgaaatgt gtggcaatgg aaggtcttat tgaagaagct aatgaggtca
    53581 tcgaaagtac cgagaaaaac gaagtgcgtg atgccgcact gattgccgca gcacagaaag
    53641 tcgagcatta tgagattgcc agttacggga cattagcgac gctggctgaa caattaggtt
    53701 accgtaaagc agcgaagctt ctgaaagaaa ccctggaaga agaaaaggcc accgacatca
    53761 aactgactga tctggccctt aataacgtaa ataagaaagc cgaaaataaa gcctgaaata
    53821 tgaattttaa cttttagtca ttttataaag aggacatttt catgaatcgt attgaacatt
    53881 atcatgactg gttacgtgac gcccacgcaa tggaaaagca agccgaatct atgcttgaat
    53941 ccatggccag ccgtatagat aattatcctg aactacgcgc tcgtattgaa caacatctta
    54001 gtgaaaccaa aaaccagatt gttcaactgg aaactattct tgatcgtaat gacatttcac
    54061 gttcagtcat taaagattcc atgagtaaaa tggctgcgct tgggcagtca atcggtggta
    54121 tattcccttc tgatgaaata gttaaaggct ctattggcgg atatgtcttc gagcaatttg
    54181 aaatcacctg ttacacctca ctattagcag cagcaaaaaa tgccggtgat acagtctcaa
    54241 ttccgatcat cgaagcgatt ttaaatgagg aaaagcaaat ggccgactgg ctgattcagc
    54301 atattccgca aacaactgag aaatttttaa ttcgttctga aactgatggc gtagaagcga
    54361 agaaataata ccctttatac catgtcctta ttgaccccgt atattacggg gtcgtttttg
    54421 tgcggaatta aaaacgatat cctgctgaga acataaacac ccacggatcg aggcgtacgc
    54481 tatcgtgttg ctgtgcaccg cctagcttat aattggcggt ggtatcgata tccatgtacc
    54541 acactgacat gttaaccaac cagtcacggt taatcagata atcaaccccc acctgcccgg
    54601 cagctcccca ggaatctttc agactgagat cggaaagccc cgcctctttg ccatgatcgt
    54661 taaatccatt atcaaagaag gtggtgtagt taataccggc cccaacgtaa ggacggaatt
    54721 tgctgctggc atcaccaaaa taccactgcg ccatcagtgt tggcggcaga tggtgaacgg
    54781 ttgcaatatc gccggtcgcc cgggtgccga ttttatggcg gaacggcgtc gctgccagta
    54841 attccacacc aatgttgtcg gtcgccatat aagtaaacgt caagcccagt tgagtgttat
    54901 tggtcacgct gaatccaccc agacttccta acgtaccacc agcaccttct gttggacgta
    54961 cggttgcaga acctgcacgc ataaaaaatt cgcctgcttc atgcgcaaag gcactgccag
    55021 aaagaagagt tgttactgcc aaagccgcca ctgttaactt tttcatatcc gctccgtcgt
    55081 tatggttata aaaacgtgac gaatatacct acattggagt aacaaatgat cctacataga
    55141 tcacattaca tatgataatt caccatttat tgatctggat taatttagaa gttgcattga
    55201 gaatcctggt taatgtgatt taaatcaatt tttaaatatt ttctctacgc aaatattaac
    55261 tcgatcgcca cttgccgcgg tttgcccacg cctgaaggcc agcaattacc tccgctttac
    55321 aaagatatgc ttttccatac aagacctgat gctgccaccc gaggcttaac cagggtacaa
    55381 ttgcccgcta attaacacct gcataaaact caaggagagt gcatgtctat cacggcgcag
    55441 tccgtatacc gtgacaccgg aaatttcttc cgtaatcaat ttatgaccat tctgttggta
    55501 tcgttgctat gtgcgtttat cacagtggtg ttagggcatg ttttctcacc cagtgatgca
    55561 cagcttgcgc agctcaatga cggcgtgccc gttagcggca gtagtgggtt gttcgacctg
    55621 gttcagaata tgtcaccgga acagcaacaa attttgctgc aggcttcagc ggcgtccact
    55681 ttttcagaat taatcggtaa cgccattctc gccggaggcg taatattaat tatccagctg
    55741 gtgtctgcgg gtcagagagt cagtgcgctg cgagctattg gtgccagtgc gccgatattg
    55801 cccaagttat ttattctgat ttttctgact acccttttag tacagattgg catcatgctg
    55861 gtggtcgttc cgggaattat catggccatt ttactggccc tggcaccggt gatgctggta
    55921 caggacaaaa tgggcatttt tgcctcgatg cgtagcagta tgcggctgac ttgggcgaat
    55981 atgcgtctgg tggcacccgc agtactgagc tggttgctgg caaaaacact gttgctgctt
    56041 tttgcctctt cttttgccgc attaaccccg gaaattggtg ccgtactggc gaacaccttg
    56101 agcaacctga tttctgccat attgctcatc tatctgttcc gcctgtatat gttgattcgc
    56161 caataacctt aagcatttga ttacggaatc gtaaaatgaa gcagtttctt gattttttac
    56221 cgctggttgt ctttttcgcg ttttacaaga tttatgacat ctatgcggct actgcagcgc
    56281 tgatcgtcgc cacggcgatt gtgcttatct atagctgggt tcgctttcgt aaggttgaga
    56341 agatggccct gatcactttt gttctggtgg tcgtcttcgg tggcttgacg ctgttcttcc
    56401 acaatgatga gtttattaaa tggaaggtga cagtcattta tgctctgttt gcgggagccc
    56461 tgttagtcag ccaatgggtg atgaaaaagc cgctaattca gcggatgctg agtaaagaac
    56521 tcacgctgcc gcaaccggta tggtcgaagc taaatctggc ctgggctgtt ttctttatcc
    56581 tttgcggtct ggcaaacatc tacatcgcat tctggctgcc gcaaaatatt tgggtcaact
    56641 ttaaagtctt tggcctgacc gcccttacct taatcttcac attgttaagc ggtatctata
    56701 tctaccgcca catgccgcag gaagataaat cctaaccaga ctgccagtcc ttcggggctg
    56761 gcgctgtatc tcctctgttc tcctcaggaa aatcatagta gcatcgcgcc tgtgattttc
    56821 cttttaagtc ggttttacca tgtctacaac acataacgtc cctcagggcg atcttgtttt
    56881 acgtacttta gccatgcccg ccgataccaa tgccaatggt gacatctttg gtggttggtt
    56941 aatgtcacaa atggatattg gcggcgctat tctggcaaaa gaaattgccc acggtcgcgt
    57001 agtgactgtg cgggttgaag gaatgacttt cttacggccg gttgcggtcg gcgatgtggt
    57061 gtgctgctat gcacgctgtg tccagaaagg gacgacatcg gtcagcatta atattgaagt
    57121 atgggtgaaa aaagtagcgt ctgaaccaat cgggcaacgc tataaagcga cagaagcatt
    57181 atttaagtat gtagcggttg atcctgaagg aaaacctcgc gccttacctg ttgagtaata
    57241 gtcaaaagcc tccggtcgga ggcttttgac tttctgctta ctgaatttcg gtggtgccgt
    57301 taattttaaa caggatattc accacaatcc cactgcctgg cttacccggc tcataacgcc
    57361 atctgcgcat cgcatttttc acctcacgct caaacatgtt cgcaggcttg gctgagagga
    57421 tttgtacgtt atccacgcga ccatccggcg tgacgtcaaa tttaacttta acctgccctt
    57481 caatgcgcaa tgcctgtgct cgtgccggat actgcggctg attacggctt aatgcgcgtg
    57541 gtcctgaagc cacactggta accggcttgc tggttgcagc cgttgccgta cttgatgtgg
    57601 ggcgcgcggg tgccgtattt tcaaacggtg atgccggacg cgactctacg ggtttgacat
    57661 cgcgttttgg ctgctcctgt acctttttca ccggcttcgg ttttggcttc ggtttcggct
    57721 tcggcttttc aatgaccacc ggtgcttctt ttggcggttc ggggatcggc tcaggttccg
    57781 gttctggctc taccaccggc tccggtggcg gctgaacggc ttgtggcggt tcgagatcag
    57841 caggcgcaac catcgtgaca gaaattggtt gcgcaggcgc aggtagttca ataacctgat
    57901 gtaccgaggt atagagcagc cccgccacaa cagcaccatg aatgcagacc gaaagtaacg
    57961 tcggccaggg gaagcggcga ggtaaatcaa gggttattga agtcataatc atttcagtag
    58021 aaaaactagg cctcgatttt aaatgcaaat agcaatcata ttcaataagg caagacgatc
    58081 aggatcagtg aaaatgtcat ttttggcgta attaccctta ctttatgcat gaccttaaca
    58141 aagacatcat ctttccgttg cagatcttcg ccctttcaaa taacgtactt tacaactttc
    58201 ccgaacaagg agttgtgccc gtgttgtatg ttatttacgc tcaagacaaa gctgatagcc
    58261 tcgaaaaacg cctttccgtt cgtccggcac atttagcacg tttacagtta ctgcatgatg
    58321 aaggtcgttt gttgacagct ggtccaatgc cagcggtaga cagtaacgat ccaggggctg
    58381 cgggatttac tggctcaacc gttattgctg aatttgaatc gctggaagca gctcaggcct
    58441 gggccgatgc tgacccgtac gtagcggcag gagtctatga acacgtttca gtgaaaccat
    58501 ttaagaaagt gttctgag
//
LOCUS       DAEEUE010000014        58070 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.014, whole genome shotgun sequence.
ACCESSION   DAEEUE010000014 DAEEUE010000000
VERSION     DAEEUE010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58070
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.014"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            698..1126
                     /gene="uspC"
                     /locus_tag="KLK84_000901"
     CDS             698..1126
                     /gene="uspC"
                     /locus_tag="KLK84_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HBE2008343.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASNPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FNLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(1133..2547)
                     /gene="otsA"
                     /locus_tag="KLK84_000902"
                     /pseudo
     CDS             complement(1133..2547)
                     /gene="otsA"
                     /locus_tag="KLK84_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
     gene            complement(2522..3322)
                     /gene="otsB"
                     /locus_tag="KLK84_000903"
     CDS             complement(2522..3322)
                     /gene="otsB"
                     /locus_tag="KLK84_000903"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HBE2008344.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(3489..4475)
                     /gene="araH"
                     /locus_tag="KLK84_000904"
     CDS             complement(3489..4475)
                     /gene="araH"
                     /locus_tag="KLK84_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HBE2008345.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGIPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(4490..6004)
                     /gene="araG"
                     /locus_tag="KLK84_000905"
     CDS             complement(4490..6004)
                     /gene="araG"
                     /locus_tag="KLK84_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HBE2008346.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(6074..7063)
                     /gene="araF"
                     /locus_tag="KLK84_000906"
                     /pseudo
     CDS             complement(6074..7063)
                     /gene="araF"
                     /locus_tag="KLK84_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            7860..8363
                     /locus_tag="KLK84_000907"
     CDS             7860..8363
                     /locus_tag="KLK84_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HBE2008347.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(8443..8694)
                     /locus_tag="KLK84_000908"
     CDS             complement(8443..8694)
                     /locus_tag="KLK84_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HBE2008348.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8809..8895)
                     /gene="azuC"
                     /locus_tag="KLK84_000909"
     CDS             complement(8809..8895)
                     /gene="azuC"
                     /locus_tag="KLK84_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HBE2008349.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            9158..9481
                     /locus_tag="KLK84_000910"
     CDS             9158..9481
                     /locus_tag="KLK84_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HBE2008350.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            9652..10149
                     /gene="ftnA"
                     /locus_tag="KLK84_000911"
     CDS             9652..10149
                     /gene="ftnA"
                     /locus_tag="KLK84_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HBE2008351.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(10186..10425)
                     /locus_tag="KLK84_000912"
     CDS             complement(10186..10425)
                     /locus_tag="KLK84_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HBE2008352.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            10615..11826
                     /gene="tyrP"
                     /locus_tag="KLK84_000913"
     CDS             10615..11826
                     /gene="tyrP"
                     /locus_tag="KLK84_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HBE2008353.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(11888..12553)
                     /gene="yecA"
                     /locus_tag="KLK84_000914"
     CDS             complement(11888..12553)
                     /gene="yecA"
                     /locus_tag="KLK84_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HBE2008354.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(12749..12835)
                     /locus_tag="KLK84_000915"
     tRNA            complement(12749..12835)
                     /locus_tag="KLK84_000915"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12799..12801),aa:Leu,seq:taa)
     gene            complement(12848..12921)
                     /locus_tag="KLK84_000916"
     tRNA            complement(12848..12921)
                     /locus_tag="KLK84_000916"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12887..12889),aa:Cys,seq:gca)
     gene            complement(12976..13051)
                     /locus_tag="KLK84_000917"
     tRNA            complement(12976..13051)
                     /locus_tag="KLK84_000917"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13016..13018),aa:Gly,seq:gcc)
     gene            complement(13203..13751)
                     /gene="pgsA"
                     /locus_tag="KLK84_000918"
     CDS             complement(13203..13751)
                     /gene="pgsA"
                     /locus_tag="KLK84_000918"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBE2008355.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(13808..15640)
                     /gene="uvrC"
                     /locus_tag="KLK84_000919"
     CDS             complement(13808..15640)
                     /gene="uvrC"
                     /locus_tag="KLK84_000919"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBE2008356.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(15637..16293)
                     /gene="uvrY"
                     /locus_tag="KLK84_000920"
     CDS             complement(15637..16293)
                     /gene="uvrY"
                     /locus_tag="KLK84_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HBE2008357.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVAGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            16589..16765
                     /locus_tag="KLK84_000921"
     CDS             16589..16765
                     /locus_tag="KLK84_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2008358.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            16752..16976
                     /locus_tag="KLK84_000922"
     CDS             16752..16976
                     /locus_tag="KLK84_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HBE2008359.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(17009..17629)
                     /gene="sdiA"
                     /locus_tag="KLK84_000923"
                     /pseudo
     CDS             complement(17009..17629)
                     /gene="sdiA"
                     /locus_tag="KLK84_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(17859..18611)
                     /gene="yecC"
                     /locus_tag="KLK84_000924"
     CDS             complement(17859..18611)
                     /gene="yecC"
                     /locus_tag="KLK84_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HBE2008360.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(18608..19276)
                     /gene="tcyL"
                     /locus_tag="KLK84_000925"
     CDS             complement(18608..19276)
                     /gene="tcyL"
                     /locus_tag="KLK84_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HBE2008361.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(19291..20277)
                     /gene="dcyD"
                     /locus_tag="KLK84_000926"
     CDS             complement(19291..20277)
                     /gene="dcyD"
                     /locus_tag="KLK84_000926"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HBE2008362.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(20382..21182)
                     /gene="tcyJ"
                     /locus_tag="KLK84_000927"
     CDS             complement(20382..21182)
                     /gene="tcyJ"
                     /locus_tag="KLK84_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBE2008363.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(21270..21821)
                     /gene="fliZ"
                     /locus_tag="KLK84_000928"
     CDS             complement(21270..21821)
                     /gene="fliZ"
                     /locus_tag="KLK84_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HBE2008364.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(21867..22586)
                     /gene="fliA"
                     /locus_tag="KLK84_000929"
     CDS             complement(21867..22586)
                     /gene="fliA"
                     /locus_tag="KLK84_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HBE2008365.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(22751..24403)
                     /gene="fliC"
                     /locus_tag="KLK84_000930"
     CDS             complement(22751..24403)
                     /gene="fliC"
                     /locus_tag="KLK84_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HBE2008366.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            24669..26075
                     /gene="fliD"
                     /locus_tag="KLK84_000931"
     CDS             24669..26075
                     /gene="fliD"
                     /locus_tag="KLK84_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBE2008367.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            26100..26510
                     /gene="fliS"
                     /locus_tag="KLK84_000932"
     CDS             26100..26510
                     /gene="fliS"
                     /locus_tag="KLK84_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBE2008368.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADVWKESLLSPSSIQDPV"
     gene            26510..26875
                     /gene="fliT"
                     /locus_tag="KLK84_000933"
     CDS             26510..26875
                     /gene="fliT"
                     /locus_tag="KLK84_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HBE2008369.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            26953..28440
                     /gene="amyA"
                     /locus_tag="KLK84_000934"
     CDS             26953..28440
                     /gene="amyA"
                     /locus_tag="KLK84_000934"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HBE2008370.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(28474..28887)
                     /locus_tag="KLK84_000935"
     CDS             complement(28474..28887)
                     /locus_tag="KLK84_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBE2008371.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            29074..30279
                     /gene="yedE"
                     /locus_tag="KLK84_000936"
     CDS             29074..30279
                     /gene="yedE"
                     /locus_tag="KLK84_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HBE2008372.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFPRE
                     APQTAKEIA"
     gene            30276..30509
                     /gene="yedF"
                     /locus_tag="KLK84_000937"
     CDS             30276..30509
                     /gene="yedF"
                     /locus_tag="KLK84_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBE2008373.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            30616..31286
                     /locus_tag="KLK84_000938"
                     /pseudo
     CDS             30616..31286
                     /locus_tag="KLK84_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(31326..32534)
                     /locus_tag="KLK84_000939"
                     /pseudo
     CDS             complement(31326..32534)
                     /locus_tag="KLK84_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            32602..33217
                     /gene="tnpA"
                     /locus_tag="KLK84_000940"
                     /pseudo
     CDS             32602..33217
                     /gene="tnpA"
                     /locus_tag="KLK84_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            33545..34627
                     /locus_tag="KLK84_000941"
     CDS             33545..34627
                     /locus_tag="KLK84_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HBE2008374.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(35116..35914)
                     /locus_tag="KLK84_000942"
                     /pseudo
     CDS             complement(35116..35914)
                     /locus_tag="KLK84_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(35924..36475)
                     /gene="ybcL"
                     /locus_tag="KLK84_000943"
     CDS             complement(35924..36475)
                     /gene="ybcL"
                     /locus_tag="KLK84_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HBE2008375.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(36644..36976)
                     /gene="emrE"
                     /locus_tag="KLK84_000944"
     CDS             complement(36644..36976)
                     /gene="emrE"
                     /locus_tag="KLK84_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HBE2008376.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(37321..37635)
                     /gene="fliE"
                     /locus_tag="KLK84_000945"
     CDS             complement(37321..37635)
                     /gene="fliE"
                     /locus_tag="KLK84_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBE2008377.1"
                     /translation="MSAIQGIEGVISQLQATAMSACAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            37849..39514
                     /gene="fliF"
                     /locus_tag="KLK84_000946"
                     /pseudo
     CDS             37849..39514
                     /gene="fliF"
                     /locus_tag="KLK84_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            39507..40502
                     /gene="fliG"
                     /locus_tag="KLK84_000947"
     CDS             39507..40502
                     /gene="fliG"
                     /locus_tag="KLK84_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBE2008378.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            40495..41181
                     /gene="fliH"
                     /locus_tag="KLK84_000948"
     CDS             40495..41181
                     /gene="fliH"
                     /locus_tag="KLK84_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBE2008379.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            41181..42554
                     /gene="fliI"
                     /locus_tag="KLK84_000949"
     CDS             41181..42554
                     /gene="fliI"
                     /locus_tag="KLK84_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HBE2008380.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWKASLQGLE
                     RIFPTVS"
     gene            42576..42917
                     /gene="fliJ"
                     /locus_tag="KLK84_000950"
                     /pseudo
     CDS             42576..42917
                     /gene="fliJ"
                     /locus_tag="KLK84_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            42914..44041
                     /gene="fliK"
                     /locus_tag="KLK84_000951"
     CDS             42914..44041
                     /gene="fliK"
                     /locus_tag="KLK84_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HBE2008381.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            44146..44610
                     /gene="fliL"
                     /locus_tag="KLK84_000952"
     CDS             44146..44610
                     /gene="fliL"
                     /locus_tag="KLK84_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HBE2008382.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            44615..45619
                     /gene="fliM"
                     /locus_tag="KLK84_000953"
     CDS             44615..45619
                     /gene="fliM"
                     /locus_tag="KLK84_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HBE2008383.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            45616..46029
                     /gene="fliN"
                     /locus_tag="KLK84_000954"
     CDS             45616..46029
                     /gene="fliN"
                     /locus_tag="KLK84_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HBE2008384.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            46032..46397
                     /gene="fliO"
                     /locus_tag="KLK84_000955"
     CDS             46032..46397
                     /gene="fliO"
                     /locus_tag="KLK84_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HBE2008385.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            46397..47134
                     /gene="fliP"
                     /locus_tag="KLK84_000956"
     CDS             46397..47134
                     /gene="fliP"
                     /locus_tag="KLK84_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBE2008386.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVINKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            47144..47413
                     /gene="fliQ"
                     /locus_tag="KLK84_000957"
     CDS             47144..47413
                     /gene="fliQ"
                     /locus_tag="KLK84_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBE2008387.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            47422..48207
                     /gene="fliR"
                     /locus_tag="KLK84_000958"
     CDS             47422..48207
                     /gene="fliR"
                     /locus_tag="KLK84_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HBE2008388.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            48492..49115
                     /gene="rcsA"
                     /locus_tag="KLK84_000959"
     CDS             48492..49115
                     /gene="rcsA"
                     /locus_tag="KLK84_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HBE2008389.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGNILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(49159..49347)
                     /gene="dsrB"
                     /locus_tag="KLK84_000960"
     CDS             complement(49159..49347)
                     /gene="dsrB"
                     /locus_tag="KLK84_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HBE2008390.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            49510..49737
                     /gene="yodD"
                     /locus_tag="KLK84_000961"
     CDS             49510..49737
                     /gene="yodD"
                     /locus_tag="KLK84_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HBE2008391.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            50035..50850
                     /locus_tag="KLK84_000962"
                     /pseudo
     CDS             50035..50850
                     /locus_tag="KLK84_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(50847..52541)
                     /gene="yedQ"
                     /locus_tag="KLK84_000963"
                     /pseudo
     CDS             complement(50847..52541)
                     /gene="yedQ"
                     /locus_tag="KLK84_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(52712..52894)
                     /locus_tag="KLK84_000964"
     CDS             complement(52712..52894)
                     /locus_tag="KLK84_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HBE2008392.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(52973..53890)
                     /locus_tag="KLK84_000965"
     CDS             complement(52973..53890)
                     /locus_tag="KLK84_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HBE2008393.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            54063..54983
                     /gene="yedA"
                     /locus_tag="KLK84_000966"
     CDS             54063..54983
                     /gene="yedA"
                     /locus_tag="KLK84_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HBE2008394.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(54972..55442)
                     /gene="vsr"
                     /locus_tag="KLK84_000967"
     CDS             complement(54972..55442)
                     /gene="vsr"
                     /locus_tag="KLK84_000967"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HBE2008395.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(55423..56841)
                     /gene="dcm"
                     /locus_tag="KLK84_000968"
     CDS             complement(55423..56841)
                     /gene="dcm"
                     /locus_tag="KLK84_000968"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HBE2008396.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(56908..57448)
                     /locus_tag="KLK84_000969"
                     /pseudo
     CDS             complement(56908..57448)
                     /locus_tag="KLK84_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(57488..57745)
                     /locus_tag="KLK84_000970"
     CDS             complement(57488..57745)
                     /locus_tag="KLK84_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2008397.1"
                     /translation="MIRGDSLWAFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLG
                     STAGKWLGALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
     gene            complement(57746..>58070)
                     /locus_tag="KLK84_000971"
     CDS             complement(57746..>58070)
                     /locus_tag="KLK84_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HBE2008398.1"
                     /translation="EKQVLVHSDQGSQYTSHEWQSFLKSHGLEGSMSRRGNCHDNAVA
                     ESFFQLLKRERIKKKIYGTREEARSDIFDYIEMFYNSKRRHGSSEQMSPTEYENQYYQ
                     RLGSV"
BASE COUNT        14600 a        14863 c        13964 g        14643 t
ORIGIN      
        1 ttagcattag aatagttgcg ataagctgca ataagcagag ccaccttttt ggtttaataa
       61 gtccttacaa atagaagggg gtctttacac ttatctaaga tttttcctaa atcgacgcaa
      121 ctgtactcgt cactacacgc acatacaacg gaggggggct gcgattttca ataatgtgtg
      181 atgcagatca cacaaaacac tcaattactt aacataaatg tataagtcat acttttgttt
      241 tgggtgtatt tcaatctgtt aaaaagtttt tcgctacgct agcaagcaaa aatgaaacag
      301 gaataatcga aatgggatgt tgcgcacggt taaaataact caccgtaaat aatcatctgc
      361 tataaataat cactttcatg caataccaga taagctattt ttaaacagac acttaccgca
      421 caacaaacta attaaataca aagaaaggca acaatcaact taatgttaat aaaatgtttt
      481 tcatatatta accacttgtt tgatgcgggg aatcagtaca ttattttctt ataattacag
      541 ttgaaatcat atgatcaccg agagtgattg attaattgtc tttactaata atcagactaa
      601 tatttacctg tttgactgag ttgggattgc gtcgtttctc cattaggagt aaagctttaa
      661 tgtcacctga agttcacagc agaataagga acaggttatg agctatagca atattcttgt
      721 cgctgttgcg gttacaccgg aaagtcagca actgctggca aaagcggtat ctatcgccag
      781 gccagtaaag ggacacatca gtttaattac tctcgcttcc aacccggaaa tgtacaatca
      841 attagccgcg ccgatgcttg aagatttacg cagcgtgatg caagaagaaa cgcaaagctt
      901 tcttgataag ttaattcagg atgcggggta tcccgttgac aagacgttta ttgcctacgg
      961 tgagttaagc gaacatattc tggaagtatg tcacaaacat catttcaatt tggtgatttg
     1021 cggtaatcac aatcacagtt tcttttcgcg agcatcctgc tcggcgaaaa gagtgattgc
     1081 ctcaagtgag gtcgatgtgc tgttagttcc acttacggga gattaaccgc tcctacgcaa
     1141 gctttggaaa ggtagcaact ttatcgcgct gctggctttc cgcgcttcgc ggaactatct
     1201 gctttaggtc gctaatgaag cactcctgcc agtggttaat atcgtttttc acgataacgt
     1261 ccagcatttc tgcatgacgg gaaatacgtt ccaccagcga catagtcaat gcacgatcca
     1321 gcgcagctgc aacttcgtca cgatcgtagg ggttaacaat taacgccgac gttaactcgt
     1381 ttgccgctcc cgcaaattgc gaaagaacaa gaacgcccgg gttggctggg tcctgagcag
     1441 caacatactc ttttgctacc aggttcatcc cgtcacgcag tggcgtcact aagcccacgt
     1501 cagagtagcg gaatattttc atcagtaatt tacggtcaaa atgctgattc aaataataaa
     1561 gcggcgtcca gcctaattgc ccgtatttac cattaattcg tccagcttca ttttcgagct
     1621 gatgacgaat atcctggtag gcttgcacat caccacgcga cgttggtgca atctgggtat
     1681 aacgaatttt accatgatgc tgcggatatt tttccagcaa tgcctcatag gcgagaaaac
     1741 gctccggcaa acctttggaa taatccagcc gttcgacaga aaagatattt tgtacgtttt
     1801 tcaattccgc tttaagttgc gccagttttg gcggcagtgg cccggcagcc tgtttggcta
     1861 tttctttcgg ttcaatgccg atcgggtaga cttctgttcg aaatgctttt ccccaggctg
     1921 tatggctttt tgcgctacgt gtcgtgacgc gggtcaggtt agaaaggcaa tccaggaacg
     1981 ccagacgatc gttttctgtc tgtaaaccca gcaaatcata atcacaaagc tgttcaagca
     2041 aggtgtcata tgtcggcagc gcgttgaaaa tttccggtgt cgggaaagga atatgcaaga
     2101 aaaaaccaat gcgattattc accccacgtt tgcgtaattc atgcgcaaag ggcaacaggt
     2161 gataatcgtg gatccagata atgtcatcgt cttgcaacag cggcagtaat ttatctgcca
     2221 gcaacgcatt tacgcgtaga tagccgtccc aggcaggacg ctgaaattgc accagatcga
     2281 gccgataatg aaaagcgggc cagagaacgg cattggagaa ttggttgtag tattcgtcaa
     2341 ggtcctgttc gctgaggtta aaagaggccc acgtaatgtt acctttttta gcggctgatc
     2401 ctcattccct gtttcaccac tccagccaaa ccacagtccg cctgcggctt tcagtgcccc
     2461 cagtatgcca acggcaaggc caccggcact ggcggcgtgc tcgtctggtg gtgcaatccg
     2521 gttagatact acgactaaac gactcatagt catcactcct gttattttct cttttttgtt
     2581 gtaatgcggc ggttatcatt tcaagccagc tccagacatc cggcacaccc gccagtcgcc
     2641 atgatgcctg agttgcacct gtgccaattt ttactgacat tccgcccagt cggttaacga
     2701 ctgcgaagcc agattcatcg gttaaatcat cgcccagaaa tacgggcgtt cgcccgataa
     2761 aaggagcttc ctgcataaag gctgcaatcg cttcaccttt actggtacct ctcggtttga
     2821 tctcgacaac acactttccc tgctgtaacg ccatttgtgg ccagatctga gtaatacgtt
     2881 gcgctaatgt cattaatgcg tcttcatgct gcggagcctg acgataatgc agcgcaaaag
     2941 ccatcccttt cgcctccagc tccgcgccgg gatactgagc gatgaccgta tgcagttgca
     3001 cgctaatatc acgcgcaatc gcatccggca gatgaacgat atgtgtttta ccattgatgt
     3061 cacggcgctc cgccccatgc acgcccgcta acgggaagcg ataaggtttt gccagtgcgt
     3121 caagctccac cattgagcgc cctgatatca atgccaatgc accatcactt gcggttgcca
     3181 gtagctgtag tccttgcaga atattgtcag gcacgacgac ctgatcggga tgcggtttga
     3241 tttccgccag cgttccatca agatcaaaaa accaggcata tttcgcggat agttcagggg
     3301 tttcggttaa cggttctgtc acccggctct cctccttctc ttttcttatt gtgttgcgca
     3361 gaccgcgctt tacagacatg taagtataga cagtgtgacg gaggtcgcca tttgcaacga
     3421 agaacagcca accgttgtgg cggctggctg tggtgggaaa aaacgttaaa ttgttgtgga
     3481 aaaaagcatc agacagtgcg cttcgctttt tgcttgtaac ggtcgaagat caccgctgcc
     3541 agcaggatta agccgcgaac cacgtactgc gcgaaaggag aaatattaag caggttcatg
     3601 gcgttttcca cggtgcctaa aattaagata cccgccacca catatgagat ttttccgatg
     3661 ccacctttca gagaaacgcc acctaaaacg caggcggaga taacaataag ctcataacca
     3721 atcgacgtca ttggctgccc actagtcata cgtgaagcca gaataattcc ggctatcgca
     3781 gataccaggc cagagagaac aaagataata attttggtgc gaacaaccgg tatacccgcc
     3841 agacgcgcag cctcttcgtt cccgccaatc gccagggtat tacgaccaaa ggtggtttta
     3901 ttcagcagca aaccaaagat aatcagacac gcgacggtga gccagattgg cgcaggcaga
     3961 ccgaaccagt tagcgtaacc aagggcaaag aagctttcat cttcgatacc gaccgcttta
     4021 ccgtctgaaa tgatgtacgc cagacctcga acaatctgca tcgttgccag tgtcgtgatc
     4081 agagcattta ttttcagttt ggcgataaca aagccattga ccaggccaca gagaacgccc
     4141 agcaacaaac ccgctgccac gccaatccac aggctttcag tcaggttgat aaccaccgcc
     4201 gtggtgacac ccgcacaggc aattacggag gcgacagaaa ggtcaaagtc accggaagca
     4261 aggcagaaca acatgccaca cgccaccatc cccgacatgg aaattgccag gcccaaccct
     4321 ttcatattaa tgaaggtggc aaaatttggg acaaaaatgg cacaggcgat aaagagcacc
     4381 gcaaacacca ccagcatgcc gtactgatcc cagatacgcc cgaagctgaa tgacgactta
     4441 ggtgcgccag accccgatgt agaaacagaa gacatcatac tctccttact caggcaactg
     4501 cctggctgac tttaggcatc gcaaggctca gtgcctgacg ctcatctgcc tgctcgtgta
     4561 acaattcacc ggcgatttcg ccttcccgca tcaccacaat ccggtcggca acgccgagga
     4621 cttcaggtaa gtcgctggag gcaaacagca ccgccacgcc ctgcgccgcc agcgcataaa
     4681 ttacgttata tatttcgtgc ttagcgccaa catcaatgcc gcgcgtaggt tcatccagca
     4741 aaatgacctt catctcttcc gataaccagc ggcccagaat ggctttttgc tgatttccgc
     4801 ctgagagatt catgatcagt tgctccgcgc ccggcgtttt gatgttgagc gaacgaatgt
     4861 gctgatcggc attgttttct tcccaactgt tgttgattac acaaccgccg agcacatgtt
     4921 tgcgtctggc actgatgttg atattgtcgc gaacggagtg cacgggaata atgccttccg
     4981 ctttgcgagc ttccgggcag agcatcatgc ctgcggcaat ggcgtggctc ggtttacgaa
     5041 tatcgatcgg ctgttggtcg atataaacct gaccggcggt gatttgcgtc ccgccaaaca
     5101 agcctttcat taattcgcta cgccccgccc ctaccagacc aaacagccca acaatttcac
     5161 cactgcgaac cgccagactt attggcgtac gcacgcctag tgctttcaca gcatcaagac
     5221 gcaggcgctc ctcgccataa ctacgaggtt gccagccgta gatatcgcca atgtcgcgcc
     5281 cgaccatcgc ctgcaccagc gcgtcgtggt caacctgctg catatcggta aaggttttga
     5341 cataacgtcc atctttaaag acagtgatgg catcgctgag ggcaaatatt tcttccatac
     5401 ggtgagaaac gtataagatc acccgcccct cttttcgcag ttcacgaata acgcggaaaa
     5461 gattgccgat ttcgcgggca gaaagggagc tggttggctc atcaaaggcg ataattttag
     5521 cgttacgcgc cagcgctttg gcgatttcaa ccatctgcca ctgaccaatg gcgagatatt
     5581 tcagcggcgt gtccgggtca atatccatac caagatgttt aagttgtaaa cccgcctcat
     5641 aattcagcaa tgagcgattc acaatgccgc ctttatgcgg cagctggccg agatagatgt
     5701 tttccgcgac ggtcatttcc ggcacgagat gcagttcctg gtaaataatc gccacacccg
     5761 cgttaagtgc tgcggtcgtg tcggaaaagg acatttcctg cccattaatc actacagaac
     5821 ccgtggttgg cgcatagttg ccgctgagga tttttaagag agttgatttt cctgcgccat
     5881 tttcacccat caacgcatga acctgaccgg catagcagtc aaaactaata tccgtcagcg
     5941 ccttaacgcc gggaaacgtt ttaccgatgc cgcgaaatga gagatacggg gtagactgtt
     6001 gcataacgtc tccgtgaatc actggtcgtt cacaccccct ctgcatcatg cagaggggaa
     6061 tttttccggc aaattactta ccgcctaaac cttttttctc cagttcttct ttaaagttgt
     6121 cacgcgtgat cagtaccacg tctgtaactt cggtaaattt tggcgtttca acgtcttttg
     6181 ctacccagtt gtaaagcatt tcgctggatt tatagccatg tacgtccggg cttggcagca
     6241 gggaaccgta gaagccggtt gcctgtgctt tagacagttc gcttactgca tccacaccgt
     6301 taataccaat accgatgata tcggctgctt taaagccctg accttcggtt gcacgtacgc
     6361 cgccaagtac ggtgctgtcg ttcataccga cgatcagcca atgtttaact tccggatgtt
     6421 gaaccagcat tgagttggca gcgtcaaatg cccccgggat gtcgttagat ttggtaggta
     6481 cctgataaat ttgtttttcc gggaatcctg cagctttcag tgcgtccata gaacccgttg
     6541 tacggcggcg ggcggtatcc agttcgttgg cggtaatcgc catcactgcg ctttctttga
     6601 catcccagcc acgtttctgc atctctttat acagttccta gccctgacgt tcgccaattt
     6661 tagtcgccgc catcatcacc agaggaacgg tatccattgg cttacctttg gcgttaacaa
     6721 actggtcatc cactgcaatg actttcatat cgtagccacg cgctttcgcg acgatggcag
     6781 agccgagttt cgggtccgga gtacaaataa cgaaaccttt tgcgccactg gctgccaggc
     6841 tgtcgatcgc gttcaatgtt ttttcgccat ccggcacggc aatcttaata acctcaaacc
     6901 ctaaatcctt cccggcttta tcggcaaact tccattcggt ctggaaccac ggctcttccg
     6961 gttgcttcac cagaaaaccg agcttcaggt tctccgccat agcggattgt gacataacgg
     7021 ctgccagacc aatggctgcc agggctttag taaatttgtg catggttctc tccagcttta
     7081 gtgtcgtttt gtgtagtgca aaaacgaatg acattcgtta aattaatcgg aaaacaaagc
     7141 attacctttt atctaaaaga taagtgaccg tgttgacata gttttagcga gaaattaatt
     7201 ctccatagga gagcaatatc acatcgcaga attacagtga gaacgtgcat aaatttagcg
     7261 ggaaaagaca taaggaaaag ccaatttgtc agacaaattg ttgaatgcac agcagattaa
     7321 tccataagat tagcctggaa atccttgttg tcttcggtac ccattcggga tgtcttcttt
     7381 ttaaccagtc aataggccgc attacctggc gttgagtttt tgaaatggtg taataaccac
     7441 aactcaaaga tgtggaaaat gcgcgtcatt catttcgcca ttaattatca ctgtgctcat
     7501 taattaacag aacacgtata atgagagcca tctcgcaaaa atgaaaaaac gttttataaa
     7561 atcatcactt catcatgaat tcaaattcat cgattaatat caacaagata caaaaagcac
     7621 tatcattaaa attcattgca tttacattga tttcaccaat gaaatgtaaa aatatataaa
     7681 cttgatgatt taagcatttt cttataaccg ttcagacgtt attcttattt cagatcatcg
     7741 tcagaattga ctccacgatc acatttcgga ccggcagaaa ggaattattc tgcaaacagt
     7801 aattatggtg ttttgattta tcttgcacct ctccacttct ggatataagg atattaggta
     7861 tggcaaccgc tggaatgctt ctcaaactca actctcaaat gaaccgcgag ttttacgcat
     7921 ccaatctcta ccttcacctg agtaactggt gttctgaaca gagtctgaac ggcaccgcca
     7981 ctttccttcg cgcccaggca cagagtaatg tgacccaaat gatgcgcatg tttaacttta
     8041 tgaagagtgt cggcgctacc cccatcgtta aagccattga tgttccaggt gaaaaactga
     8101 actctctgga agaactgttc caaaaaacga tggaagaata cgagcaacgt tcaagcactc
     8161 tggcgcagtt agccgatgaa gcgaaagaac tgaatgatga ttcaaccgtc aatttcctgc
     8221 gcgatctgga aaaagaacag cagcatgatg gcctgttgct gcaaaccatt cttgatgaag
     8281 tgcgcagtgc gaaacttgcg ggtatgtgcc ctgtgcagac cgaccaacat gttctgaatg
     8341 tcgtgtcaca ccagctgcat tgatcataca tcggcgctaa tacattgcgc cgatgaagtt
     8401 tttgagaaac cgctgcctca tctgtttgaa gcagcggttt ttttaatggg attcaccctg
     8461 tggggtaaac ttgagttcaa tcagcgcgat ggctttttgg attgcccgca tggtgaccgg
     8521 gtctgcggca gcgggatgat gagtaaagtc gatattcttc agctgactgg acattttttc
     8581 acgaacttca acgggcgcga ttacatcgag aacatccaga atttgtttga taaccaactg
     8641 gcaagctacc acatcagaaa ccagttcctg atcggcattc agcggctggg acatcgtaaa
     8701 ctcctgatag cattttgaaa gccgttatag tagcggcttc acatcttcag cgatagtcac
     8761 atccaccgtc atcaggacac aaaaaaacct gccggagcag gttttttgtt atcggaacat
     8821 attgcctggc ggtacgtctt tgaacgtctt gcaatagtta ttgaacatac ttttcaggat
     8881 tttgcgcagt ttcatcgcgg cactccgacc atttgttata caggtgttat tgtctttgcg
     8941 cttataatat gacaaccatc acaaaaatca atctttatgt gatacaaatc acataaatac
     9001 ccctttaatg ttataaaaat gataatcaaa aaacagcccc cctatttctg acacctacag
     9061 atggcaagaa atagcgcctg ccaggcgtct tttccggcca ttgtcgcagc gctgtaacgc
     9121 gtaaaatagt gctttctctt actcttctgg ctggaccatg agacttctga ttctgactct
     9181 ttcattaata acgctggcgg ggtgtacggt tactcgccag gcccatgtga gcgaagttga
     9241 cgcggcaaca ggtattgtac ggttggttta tgaccaggct tttttgcagc atgcccatac
     9301 cgatcgttat gtcagtcgcg ggatcgccga tcgcgcatgc cagcaggaag gctataccca
     9361 cgcgatcccc tttggtcagc cagtaggcaa ctgtagcctt tttgcgggtt ccttatgttt
     9421 gaatacggaa ttcactttgt catatcagtg ccatcattcc gccttccctg tctttctcta
     9481 aatattttaa tcagcgaggg gatcttcgct gattaaagaa atagcggtaa tgcgttttat
     9541 tcccattcgt atttttaata attgaagttt atattttacc ttttgcaaat aataaaataa
     9601 caaattatag tgacgccaca gcaacaaata taacctttgt ggagcactat catgctgaaa
     9661 ccagaaatga ttgaaaaact taatgagcag atgaacctgg aactgtactc ttcactgctt
     9721 tatcagcaaa tgagcgcctg gtgcagctat cataccttcg aaggtgctgc cgcgttcctg
     9781 cgccgtcacg cccaggaaga gatgacgcat atgcagcgtc tgtttgatta cctgactgat
     9841 accggcaatt taccgcgtat taataccgtt gaatctccgt ttgctgaata ttcctcactt
     9901 gatgaattat tccaggaaac ctataaacac gaacaattaa tcacccagaa aattaacgaa
     9961 ctggctcatg ctgcaatgac caatcaggac tacccaacat ttaatttcct gcagtggtat
    10021 gtttctgagc agcatgaaga agagaaactg ttcaaatcga ttattgataa attaagcctg
    10081 gcaggcaaaa gcggcgaagg tctgtatttt atcgacaaag aactctctac cctcgacaca
    10141 caaaactaat gctctccggc ggcaggtttc tgccgccgtt tcaaatcagt gacggcaaat
    10201 cttactctga tcggtcgaaa aaccttcttc tgaaggtata aatttgcctt ttgctgccag
    10261 aaacgctacc agctctcccg ctgtcacccc ttctgccgag cacgtgtgaa aacgtgcctg
    10321 ttcaccaaaa cgcgctttaa tcgcagcttc cagactggca tgcgtatatt gctcgcctga
    10381 ttcaatcatc atatttaaca cttcatgacc gtgaatagag tccatcgttc ctcctcaaaa
    10441 aaagcctagc gtagcgatta ccgcttatga agactttgcg ccagcgcagg actgaatgct
    10501 tttattgtac atttatattt acacaatatg taacgtcggt ttgacgaagc agccgttatg
    10561 ccttaacctg cgccgcagat atcactcata aagatcgtca ggacagaaga aagcgtgaaa
    10621 aacagaaccc tgggaagtgt ttttatcgtg gcgggaacca caattggcgc aggcatgctg
    10681 gcaatgccgc tggctgcggc cggtgttggt tttagcgtta cgttaatctt gttgattggg
    10741 ctttgggcgt tgatgtgcta cacggcgcta ttactgctgg aggtgtacca gcatgttccg
    10801 gcagataccg gtctgggcac gctggcaaaa cgctatctgg gacgctacgg tcaatggctg
    10861 acgggcttca gtatgatgtt cttaatgtat gctctgactg cagcatacat cagcggtgcc
    10921 ggtgaattgt tggcctccag catcagcgac tggacaggta tttcaatgtc ggcaaccgct
    10981 ggcgtgctgt tgttcacttt tgttgccggt ggcgtggttt gtgttggaac atcgctggtc
    11041 gatttattta accgttttct gttcagcgcc aaaattattt ttctggtggt gatgctggta
    11101 ttgctgctac cgcatattca caaagtgaat cttttaaccc tgccgttgca acaggggctg
    11161 gcactgtctg caatcccggt gatttttacg tcgtttggtt ttcacggtag cgtgccgagt
    11221 attgtcagct atatggatgg caacattcgt aagctacgct gggtgtttat aatcggtagt
    11281 gccatccccc tggtggcata tattttctgg caggtggcga cgcttggcag cattgattca
    11341 acaaccttta tgggattgct ggctaatcat gctggattaa acgggctgtt acaggcgtta
    11401 cgcgaaatgg tggcctctcc gcatgttgag ctggcggtgc atttatttgc tgatttagcc
    11461 ctcgccacgt catttctcgg cgttgcgtta ggcttatttg attatctggc tgatttgttt
    11521 cagcgttcaa ataccgttgg tggacgattg caaactggtg caattacctt tctgccgccg
    11581 ttggcgtttg cactgtttta tccacgagga tttgtgatgg cgctgggtta cgccggtgtg
    11641 gcgctggcgg tactggcatt gattatccct tcactattga cctggcaaag cagaaagcac
    11701 aatcctcagg cgggttaccg ggtcaaaggt ggtcgtccgg cgctggtggt ggtgtttctc
    11761 tgtggtattg ctgtgattgg cgtgcaattt ttgattgcgg caggcttgtt accagaagtg
    11821 gggtgatcag atagcctcaa attccttatt gggtgccaga attaacgctg acacccaatg
    11881 tgacctctta atgcaggcag cactgcttaa atttcttacc actgccgcac ggacaaggat
    11941 cgttacgccc cggtttctct tctgctttga tcggttgctg aacagctttt tcctgcggat
    12001 gcgccatcca gtatgcatgt aaatcaagcg ccgccagtcg aatggcatct acgctctctt
    12061 caaacgcttc tggcgacatt ttctctaccc gctcgaagtt ttcctcagta ccgtgcagcg
    12121 caatcgcctc cagcgctggt tttaaagaat cgggcaacgt tgaccagtca gaaagtgcca
    12181 cgccccgcat atagccaaag caccactcct caacaatcgt cagctcgctg ccatcaactt
    12241 ctcgcaagcc gaataacggc tcaaactgct ccgggaattc gttcagacgc tctgcggtat
    12301 cggccatatg ttgaaaagcc aggttcataa agcgcgtcat ctctttctct gacgcccaac
    12361 gcggcacata gtcagcccca ccccacacgg caaccagcca ctgttctggt tcaatctctt
    12421 gcggagaact caacaccgcc gtcaataaac cgtccagctc cgccacatca aggatggcgt
    12481 ggtcagtgtt gtatttggtc agaatatcgt ccagccattc caactcactt tcgtttaacg
    12541 gtcccgtttt catacgcttt tccttgtgga tctcaactcg ccagcaccta tcttacatgc
    12601 cggtccgtat cagagatact ttttgagtgg ctttgccggt gattaaaaat taaggagggt
    12661 gtaacgacaa gttgcaggca caaaaaaacc acccgaaggt ggtttcacga cactgcttat
    12721 tactttgatt ttattcttat ctttcccatg gtacccggag cgggacttga acccgcacag
    12781 cgcgaacgcc gagggatttt aaatcccttg tgtctaccga ttccaccatc cgggctcggg
    12841 aagaaagtgg aggcgcgttc cggagtcgaa ccggactaga cggatttgca atccgctaca
    12901 taaccgcttt gttaacgcgc caaattcttc aggcctttca gccagacatc cgcttgacgc
    12961 cgatgtcttt taaactggag cgggaaacga gactcgaact cgagaccccg accttggcaa
    13021 ggttgtgctc taccaactga gctattcccg cattcatcaa gcaatcagtt aatcacttga
    13081 ttttattatc gtctggcaat cagtgccgcc gttcgatgcg ttgcattcta cttacctggc
    13141 gcgatgagtc aacgatattt ttcaccactt ttgatcgttt gctgaaaatt acgccgaaac
    13201 gatcactgat caagcaaatc tgcacgcgca gcgctcaaat attgcaacat tgaccacaga
    13261 gtcagtaccg cagccacaaa gaaaagtgca ataccgacgt actcaaccca aatgttcgga
    13321 cgccacagca gccaggccaa cgccaccatc tgggcagtgg ttttcacttt cccaatccag
    13381 gagacggcca cgctactgcg tttacccaac tccgccatcc attcgcgtag cgcagaaata
    13441 ataatttcac gggcgatcat cgttgccgcc ggtaatgtca cccaccagct gtgataatgc
    13501 tcggttacca gcaccatggc gatagccacg agaactttat ctgccacagg gtcaaggaaa
    13561 gcaccaaacc gggtactctg gttccagcgg cgtgccagaa aaccatcgaa ccagtcagtc
    13621 accgccgcga cgcagaaaat gagcgcggcg gcaaacggcg accaggtgac aggcagataa
    13681 aagaccaata caaagaatgg gataaggatg acacggaaca gtgtaagcaa cgtagggata
    13741 ttaaattgca taatgacggg taactatctg ttgtcagtaa gattacccct atgttgctac
    13801 agagacatca atgtttcaac gaccagaaga tcttttctgc cagaccttgc gaaatacccg
    13861 gcacttttgc aatttcctcg acgctggcgt tacgtaaacc ttgcaaaccg cccatatatt
    13921 tcaacaacat ttgccgacgt tttggcccga cgccttcaat ggtttccagg gaactggtat
    13981 ttttgacctt cgcccgtttt ttacggtgcc cgccaatcgc gtgatcatgt gattcatcgc
    14041 gaatatgctg gataacatgc agcgcgggtg aatctggcgg caaactaaat ccctcacctt
    14101 ccggctcaaa gaacagcgtt tccagtccag ccttacgatc tgctcctttg gcaacgccaa
    14161 gtagcagcgg atgattttta tcccatgaga catccagttc ggcgaagaca tttttcgcct
    14221 gcgcaagctg gcctttgccg ccgtcgataa ggatcacatc cgggatctta ctgtcgtcaa
    14281 tggctttacc ataacgccga cgcagcacct gattcatcgc cgcataatca tcgcccggcg
    14341 tgatgcctgt aatgttatag cgccgatact ccgcacgcag cgggccgtta gcatcaaaca
    14401 ccacacagga agcgacggtt tgttcgccca tggtatggct gatgtcaaag cactccatcc
    14461 gcttcacttc cggcaatttc aacacgctgg caagcgcggt cagtcgctgg tgaacggtag
    14521 attgctgcga aagtttgctg gttaaggccg tcgccgcatt ggtgcgcgcg agtttcagat
    14581 aacgcgccct atcgccgcga ggtttggttt gaacattaat cttgcgtccc gccagttctg
    14641 aaagggaatc ggcgagcagc gttttatcgc taagattaaa atcgagcagg atctcacccg
    14701 gtaaggtgcg catctggctg ccttgtaaat agaactgacc tacgaaggtt tctaccacct
    14761 cgctcagttc cgtaccgcca ggcactttcg ggaaatagct gcggctgccg agcactttgc
    14821 cctgacgaat gaacaatacg tggacacaag ccatgcccgc atcgaacgcc acaccaataa
    14881 cgtcaaggtc gtcgccggta ttggaaacaa actgtttttc ggtgacgcgt cgcaccgcct
    14941 gaatttggtc gcgaatacat gccgcttctt caaactccag attctggctg gcagtttcca
    15001 tacggctaat gagttgcgta agcacctgat catctttgcc agacaaaaac aggcgcacat
    15061 actcgacctg ctgagcgtat tcttcttcac tcaccagtcc ttcaacgcac ggtcccagac
    15121 agcgccctat ctggtattgc agacacggac gcgagcgatt gcgataaaca ctattttcgc
    15181 actggcgaat ggggaaaatc ttttgcagta gcgccagtgt ttcacgtacg gcatagccat
    15241 tcgggaacgg gccgaaatat tcacctttgg catgctttgc accacgatgc atcgccagac
    15301 gcgggtgggt atcgccactc aggaagataa aaggataaga tttatcatcg cgtagcaaaa
    15361 cgttgtaacg cggctgatag agtttgatgt agttgtgttc cagcaacagc gcttcggttt
    15421 ctgtatgcgt aaccgttaca tcaatttgct ggatctgggc gaccagcgct tcggttttgc
    15481 gcgaagcgag gttgctgcgg aaatagctgg aaagccgttt tttcaggtct ttcgctttgc
    15541 cgacatagat aaccgtacca ccagcatcgt acatgcgata aacgcctggc tggctggtta
    15601 cggtttttaa aaacgctttt gcgtcaaact gatcactcac tgacttgata atgtctccgc
    15661 attacacaga ccatggcgaa ttgccaggtg agtcagctca acatcgccat gaatgtttag
    15721 tttactgaac atacgatagc ggtagctgtt caccgttttc ggactgagat tgagctgttc
    15781 tgagatctca ttgaccttct ggcccttagt gatcatcagc ataatctgca attcacgttc
    15841 agacaaactg gcaaatgggc tttctgtttt ttctggttcg atctggctta acgccatttg
    15901 ttgagcgata tcagaagcaa tgtaacgctg ccctgaatag acagaacgaa tcgcactcac
    15961 gacttcctgc ggagccgcgc ctttgctgag gtagccagca gcacctgcct gcatgacttt
    16021 cgctggtaaa gggttttctg tatggaccgt aagcatgatg attttaacat cagctgtgga
    16081 acgcgcgatt ttacgcgtcg cctcaagacc gccaatgccc ggcatactca tgtccattag
    16141 caccacgtca acggcatttg tccggcacca cttaacggcg tcttcaccgc acgatgcctc
    16201 tccggcgact tttataccct taatatcttc cagaatgcgt cgtatccctg cgcgcaccag
    16261 ttcgtggtca tcaacaagta gaacgttgat caaaggaata tctccagaaa tagggataac
    16321 gctactgata gttagtcatt cgtatattag cggtttttat tgcaactttg aaacgttaaa
    16381 aatggtgggt attcgatttt tctctcgttt ttgtcaattc gattgtccac agttatggtc
    16441 acgcccgtca ccaacggctt accggatcgt ttcacagtaa gccctacgat gaaatgtgac
    16501 aaaaattgac tttattcagc aaaaatgaaa atcagcccgc tgaatatgta acattaatta
    16561 acccgaacaa tacgggcaaa aaacattcat gataaaaata tttattggtc attatattaa
    16621 cgtattttat agcactgccg atatcacgct caaaaaacaa ccactgctat ttttagcaaa
    16681 gcttatggta tactccgccg ccttaacatt tttcaccaca aattttcatt gcaacatgac
    16741 gaggaaaata aatgagtacg cctgattttt ctactgccga gaataatcaa gaactggcaa
    16801 atgaagtctc ctgcctgaaa gcgatgctga cgctgatgct gcaggcgatg ggacaagctg
    16861 acgcgggccg cgtgatgtta aagatggaaa aacagcttgc gctgatcgaa gacgaaaccc
    16921 aggctgcagt attttccaaa acggttaagc aaattaaaca agcctaccgt cagtaatata
    16981 aaaccggctg agagcgtgcc tttcagccgg ttattgacaa aatcatcgct atctctgctg
    17041 atgtcttccc ttccgccgtc catttcagaa tttctttttc gcgcttgctg aaattcatct
    17101 ctggcgtcat cactatttca tcatttaaac gcatcagagc catcagactt tcgcgcacca
    17161 gtaactgcat ttttaattgc agttcatcac taagaatggg tatttcgcgc gtgctgcaac
    17221 gggaaaagga caaaaagccc agcgcccgat ttggcagcat taaatactga gtgacaccgc
    17281 ggcgtaaacc atgtgcgtgc gcggcttccc ataatggctg tgcttcgctg aataagtcat
    17341 cattccacat taaatggccc tgactaaagt tttcagggtt cagcacagga tcaattgcga
    17401 gaaaattttt tgcctgataa taactaaccc acgactcagg gtaattggtg tagaaagcca
    17461 ctttaggtcg cgtgaatggt accgggtggc gaacacataa cgaatagtaa tcgtactcca
    17521 gctgctgagc ctgaagctct atttcatggt aggcctcttc tgcggcctcc atcctctgaa
    17581 aacgcaacag catcgtgcga cgccagctga aaaaatccgt atcctgcata gtaaaccgca
    17641 acgcccctga gagtgagtat catttataat gataattcaa actatcacat aaataagatt
    17701 tatatataat ttatattatt caggcaatga attacttttg caagccatcg cattctctta
    17761 tgttattaat gagttatgct gatttgttaa gcagttttat cacgcttgaa atggcgtccc
    17821 gccccgacag gtgaatcgtc ggggctgatt ttttcttatt attgcagcag aagcttctcg
    17881 aggaactggc gggtgcgagg ctgctgggga tcggcaaata acgcttttgc tgccccctgc
    17941 tcgactatcc gcccctggtc cataaagatc gcccggtccg caacatcccg ggcaaagctc
    18001 atttcgtgtg tcacaatcac catcgtgcgc ttttcctgcg ccagctggcg gatggtgttc
    18061 aggacttcgc ccaccagctc tggatccagc gccgacgttg gctcgtcaaa caaaatcacc
    18121 tcaggacgca ttgccagtgc acgcgcaatc gcaacacgct gctgttgacc gccagacaaa
    18181 cgacgtggat agctggtttc tttaccggcc agcccaactt tcgccagcag ctcgcgagcg
    18241 cgcgccgtgg cctcttcttt cggttcgcct ttgacgatca ccggcccttc aataatgttt
    18301 tccagcaccg tacgatgcgg aaacaaatta aagttctgga agacaaaccc gacgtgctga
    18361 cgcaactggc gaatcagcga tttttgctga cttaatgaac gtgcagtatc aatagtgata
    18421 tcgccaacgg tgatcgttcc cgcttccggt tgttccagca gatttatgct acgtagcaat
    18481 gtggttttgc cggaaccact cggaccgata attgccacca cttcgccagg ctttacctcg
    18541 aggtcgatac cgtgcagcac cgtctgaccg tggaattttt tcaccaggtt cttaacttca
    18601 atggcactca ttttggttct ctctcctggc gattaagttg attctcaaaa tggttctgca
    18661 acgtcgataa cacagtcgcc atgatccagt agatgagcga agccgccagg tacatggtga
    18721 acacctccag cgtgcgcgag gtaatcaact gcgcctgacg gaacagctcc ggcacctgga
    18781 ttgtcgcggc cagcgacgta tcttttacca gactgataaa gctgttcgac agcggcggca
    18841 acgccacccg cgccgcctgt ggcaaaatcg cgcgacgcat ggtctgccac ggcgtcatac
    18901 caatactggc agcggcttcc cactgacctt tatcaataga agaaatggcg gcgcgcagcg
    18961 tttcggcggc ataggcggca gtgttcagcg acagaccaat cattgctgac ggaatgggat
    19021 ctaattcgat accaaactgc ggcaggccgt aatagatcat aaacagttgt gcgatgagcg
    19081 gcgtaccacg aaaaatagag atataaaaac gcgccagcca gcgcacgggc cagattggcg
    19141 acaggcgcat cagcgcgaga ataaacccga gtagtaagcc aaaaaacatg ccgccgatac
    19201 tgagttgcag ggtataccca gcccctttca acaggaacgg caaggaatca ataaccagtt
    19261 gtatactttc ttgcatgagc gttcccggag ctaaacgtgg ggatgatagg cgaacagcgc
    19321 aggcgcgccg ccggtatgaa taaacagaat cggcccttca tctttgaagc gtttctgact
    19381 gataccgtca atcagccccg ccatcgcttt tccggtatac acaggatcaa gcagaatgcc
    19441 ttccagacgc gccagcaatt tcactgcttc catgccttcg tcgttcggca cgccgtagcc
    19501 aggtgcaaaa tagtcatccc agagtaaaat ttccgctgat gcggtcagct ccagttcttt
    19561 cgcaatcgcc tgttgtaggt taaccacttt cggcaattga tcggcaacgg aacgcgacac
    19621 ggtcacgcca atcagttcgc tttcaggcat caggtgttcc agcccaacag ccagtccggc
    19681 gtgagttccg gcactgcccg atgcgactac caccgacgaa atattaaccg ccccttcaca
    19741 ctgttgcgcg atttccagcg cactctccac ataacctagc gcgccaagag cattagaacc
    19801 gccaaccgga atgacatacg ggcgaaagcc ttgtgcttcg actcgcgtcg ccagttcttc
    19861 cagttgggca ttgggatcgg tcagtgcgtc gcacatttca atctgggtat tgaacagatc
    19921 caacaacaaa cgattgccgt tggttaaata gttttctgcg gttgtgccaa taggattttc
    19981 cagcagcgcc acgcagtgca gaccgagttt cgccgcgact gcggcagtct ggcgcacatg
    20041 gttagactgg atcgcgccgg cagtaatcag cgtatcggca ccttcgcgca gggcatctgc
    20101 cgcgagaaat tccagcttac gtaatttatt gccgcccatt gccatggggg tgacctcatc
    20161 ccgtttgatg aaaatttccc gtccaagata atcagaaaag cgcggcagat attcgagcgg
    20221 cgttggcgcg ccgataaact ccaaccgtgg aaaacgggtt aaattatgca gtggcataac
    20281 agcctccgat gtgtgttgtt gtgattttct tattatgcac gctgaaaacg cgtaaataaa
    20341 aaaggcgcta gtgaaagcgc ccttttttgt cattatgctg attatttggt cacatcagca
    20401 ccaaaccatt tttcggaaag agcttgcaga gtgccgtctt tttgcatttc cgcaattgca
    20461 tcattcaccg ctttcagcag gtcttcattt cctttacgca gcgccacgcc agactcctga
    20521 cgggagaatg cttcaccggt tactgccagc gtatcgttgg ttttcttcac cagatccagc
    20581 gccgccagac gatcaacgag gatcgcatcg atacgcccta cgcgcagatc ctgatatttg
    20641 gtcgggtcat catcataggt acgcacatcg acaccctgaa cattctgccg cagccactct
    20701 tcatagttgg tgcccagacc gacacccact tttttgcctt tcagatcagc agccgtttta
    20761 atggtgcctt cgttaccttt tttcaccagc gcctgaatac cggaaatggt gtaaggggta
    20821 gagaaatcgt attttttctt gcgctcgtca gaaatggtga cctgattaat caccacatca
    20881 atgcgtttag agtccagcga cgccagcata ccgtcccatt tggtcggttt tagtgacgcc
    20941 tcaacgccaa gatgttttgc cagctgttgg gcaaattcca cttcaaaacc ggttaatttg
    21001 ccgtcatccc cctgaaaact gaacggcgga taagtgcctt ccagccctac cagcagcgtg
    21061 ccgctctctt tgactttatt aagcagacct tcatctgcaa aacttttaac gctcatgccc
    21121 gcaaccagcg ccacggccat cacacccatc aatgcctgac gtcccagatg tgctaatttc
    21181 atattcaccc cgaatgttgt tatgtctgtt tgcagtgtag agccattgtt tgtaaacaca
    21241 aaaacaactc cgctacatct tattcttatt taatatatat cagaagcagg caggctggag
    21301 gatttctgca caagtccggt acaggtttgg cgctgatagt gttgatactt ccgtaatgcg
    21361 atgcggtaat tgttggtgct ggtggtgggc agccacgggg cgaggatttc atcaaggaaa
    21421 ccgtcctgca gctgatcgag ggaaatattt tgctcatgta gatgattacc caaacggcgc
    21481 agacgaacga catattcacg caccgtacct gggctcattt cagtttgctc aaaaagaaat
    21541 ttcttaaagc cgataatatc gaaaaaatca ctctgcgttt tgcaatgtaa atcaccgcaa
    21601 aatcggcaca atgccgccca tgattgttgt tccgtttgcc agcctttttc atcaagcagc
    21661 gtgtccaggc gggaaatctc gattttattc actattttgc cgtcgcgaac taaggtaatg
    21721 cgatcgagta atttacggca atgcgcgcaa tgggtctggc tgtgtttaaa gtctttaaga
    21781 tagcggctta atggccgtct tttcaggtgc tgcaccatca ttaagaactc ctggtagtca
    21841 aagttaaagt gcggcattta ctgacgttat aacttaccca gtttagtgcg taaccgttta
    21901 atggcctggc tgtgtaactg actgacccgc gattccccga cctccagcac cgcgccaatc
    21961 tctttgagat tcagctcttc ctggtaatag agggttaata ccagtttttc gcgctccggc
    22021 aacgtttcga tggcttccat cacccgctgg cgcagattac tgtccagcag ttgttgtagc
    22081 gggttttctc gctgatgatc atcagtaacc agttcgatgc tatcgccgtg ctcttcacgc
    22141 cactcatcgt aggagaagag ctggctgtta ttggtgtcga gcagcatttg acgataatcg
    22201 gcaatatcga tccctaaacg ttccgctacc tcagtttccg tggcgttgcg gccaagttcc
    22261 tgctccagtt gccctattgc ctgtgccact tcacgcgcgt tgcgtcgcac gctgcgcggc
    22321 acccagtcac ggctgcgaag ttcatccagc atagcgccac ggatacgctg cactgcgtaa
    22381 gttgtaaatg ccgttccttg tagggcgtca tagcgttcga cagcattaag taacccaatg
    22441 ccgcccgcct gtagcagatc gtcaagttcc acgctcgcgg gcagtcgaac ctgcaggcgc
    22501 aatgcttcgt gacgcaccag cgggacataa cgctgccaca gcgagtgttt atccattaca
    22561 ccttcagcgg tatagagtga attcacgata aacagccctg cgttatatga gttatcggca
    22621 tgattatccg tttctgcagg gtttttaatc ggacgattag tgggtgaaat gaggggttat
    22681 ttgggggtta cgcaaaaagc cgaatgtaaa aaaccccgcc ggtggcgggg tttgagaggc
    22741 attcagacga ttaaccctgc agcagagaca gaacctgctg cggtacctgg ttggcttttg
    22801 ccagcacgga gttaccggcc tgctggataa tctgcgcttt cgacatgttg gacacttcgg
    22861 tcgcatagtc ggcgtcctga atacgggact gcgcttcaga caggttggta gtggtgttgt
    22921 tcaggttggt gactgcggaa tccaaacggt tttgcaccgc acccagggag gagcggaatt
    22981 tgtcgatgga gctgatggct tcatccagag ctttcagggg atcggctgtc gtagctgctt
    23041 ttgtttcagc atctgtcgtc aatttaccat cagcatcttt ataaactgcc ttaccgctgc
    23101 cgttagtcac tgaaccatca tcctgtaggt aaaaatctac agtatcagat gtggtcaacg
    23161 cctcgttgtt atcaacataa accgaatcaa caccttttgt tacagcgcca gttgtgccgt
    23221 tgacgctata acctgcagta gtgaaatcaa cattgtttcc cgaggttgat acagctttat
    23281 cgaaagctgc ctgatcaact tttgcacctg ttactgaata agtaatagtg tccggcgtag
    23341 tactattacc ggtaaaggag atagaggtac cgccaatgtc aatggttgtc gctttggtat
    23401 tattggctgc aagtgtggac agactagcct cagtcaaaga agcaccagag ccgtttttcg
    23461 ttaagcgccc agttgtatca atgtagagtt tatctccatt gcttgccgta attttaccat
    23521 cactgctgat ctgaacatcc tgtgcagaac catcgatagt aatagtgccc ttagcggtat
    23581 caccaactcc cggggtcaaa tatttctgaa catcggcagc tgaagccgtt gtggtatcaa
    23641 aagagtatga cttacttgca ctgtcatact tgtaattcgt tgcactagcc gccgttccga
    23701 agccgttatt aatggttgcc tgaacagtat caccatcact aactccagcc agcaaatcag
    23761 acgctttagc agcatcgtaa cccgcactca cagtatattt gttgccgacc actgttgcat
    23821 tagcagctac caagtcagct ttagatgcag cagtattagc cacagccccg ccaccattca
    23881 cattaaaccc attcagcccc agcgtatcag agtcaatttt cttcagatca atagtgatag
    23941 tctggccgtc attcgcacca acctgaattt tcatcgaacc gtcttttgcc agtacgttca
    24001 cgccgttgaa ctgggtctga ccggatacgc ggtcaatttc gtcgagacgg gatttgattt
    24061 cgtcctgaat ggagtccaga tccgaatcag agttagtccc ggtagaagcc tgaaccgtca
    24121 gctcacggat acgctgtaag ttgttgttga tttcggacag cgcgccttca gtggtctgtg
    24181 caacagaaat accgtcgttg gcgttacgtg cagcctgagt caggccttta atgttagaag
    24241 taaaacggtt agcaatcgcc tgacccgcgg cgtcatcctt cgcgctgtta atacgcaagc
    24301 cagaagacag acgctcgata gaactcgaca gcgcagactg gttcttgttg atattatttt
    24361 gagtgatcag cgagaggctg ttggtattaa tgacttgtgc catgattcgt tatcctatat
    24421 tgcaagtcgt tgattacgtt ttgggtttct acccgtcggc tcaatcgccg tcaaccctgt
    24481 tatcgtctgt cgtaaaacaa cctttagaat ttttttcaca aacagccatt ttttgttagt
    24541 cgacgaaata ctcttttctc tgccccttat tcccgctatt aaaaaaaaca attaaacgta
    24601 aactttgcgc aattcaggcc gataaccccg gtattcgttt tacgtgtcga aagataaaag
    24661 gaaatcgcat ggcaagtatt tcatcgctgg gagtcgggtc aggtctggat ttaagttcaa
    24721 tccttgatag cctcaccgcc gcgcaaaaag cgacgctaac ccctatttca aatcagcaat
    24781 cgtcgtttac cgctaaactt agcgcctacg gtacgctgaa aagcgcgctg acgactttcc
    24841 agaccgccaa tactgcattg tctaaagccg atcttttttc cgccaccagc accaccagca
    24901 gcaccaccgc gttcagtgcc accaccgcgg gtaacgccat cgccgggaaa tacaccatca
    24961 gcgtcaccca tctggcgcag gcgcaaaccc tgaccacgcg caccaccaga gatgatacga
    25021 aaacggcgat cgccaccagc gacagcaaac tcaccattca acaaggcgac gacaaagatc
    25081 cgattaccat tgatatcagc gcggctaact catcgttaag cgggatccgt gatgccatca
    25141 acaacgcaaa agcgggcgta agtgcgagca tcattaacgt gggtaacggt gaatatcgtc
    25201 tgtcagtcac atcaaatgac accggccttg ataatgcgat gacactctcg gtcagcggtg
    25261 atgatgcgct acaaagtttt atgggctatg acgccagtgc cagcagcaac ggtatggagg
    25321 tctcggtcgc cgcccagaat gcgcagctga cggtcaacaa cgtcgccatc gagaacagca
    25381 gcaacactat cagcgacgcg ctggaaaaca tcaccctgaa cctgaacgat gtcaccacgg
    25441 gcaaccagac gctaaccatc actcaggaca cctccaaagc gcaaacggcg attaaagact
    25501 gggtgaatgc ctataactcg ctaatagata ccttcagcag cctgaccaaa tacaccgccg
    25561 tagatgcggg agctgatagc cagagttcta gcaatggcgc actgctcggc gactccacgc
    25621 tgcggacgat tcagacgcag ttgaagtcga tgctgagtaa taccgtcagt tcttccagct
    25681 ataaaacgct ggcgcagatt ggtatcacga ccgatcccag cgatggcaaa ctggaactgg
    25741 atgccgacaa actcaccgct gcactgaaaa aagatgccag cggcgtaggt gcattgattg
    25801 ttggcgatgg taaaaaaacc ggcatcacaa ccaccatcgg cagcaacctg accagttggc
    25861 tttcgacaac gggcattatt aaagccgcta ccgatggcgt tagtaaaacc ctgaataagt
    25921 taactaaaga ctacaacgcc gccagcgatc gcattgatgc gcaggtcgcg cgctacaaag
    25981 aacaatttac ccaactggac gttttaatga cctcgttaaa cagcaccagc agctacttaa
    26041 cgcagcagtt cgaaaacaac agtaattcca agtaatcatt attcatcggg agacaggtca
    26101 tgtacgcggc aaaaggcacc caggcctatg cacaaattgg cgtcgaaagc accgtaatga
    26161 gcgccagcca acagcagttg gtcaccatgc tatttgatgg agtgctgagc gcactggtta
    26221 gagcgcgact gtttatgcag gacaacaatc agcaaggcaa aggcgtctct ttgtcaaaag
    26281 cgatcaacat cattgagaac ggactgcggg tgagtcttga tgaagagagc aaagacgaat
    26341 taacccaaaa cttaattgct ctttatagct atatggtcag gcgtttgctg caagccaatt
    26401 tacgcaacga tgtctccgca gtcgaagagg tggaagcatt aatgcgtaat attgccgatg
    26461 tctggaaaga gtctttactc tccccttctt cgattcagga cccagtctga tgaacaatgc
    26521 accgcatttg tatttcgcct ggcaacaact cgtcgaaaaa agccagctca tgttacgcct
    26581 ggcaacggaa gaacaatggg acgaactcat cgctagcgaa atggcgtatg tgaatgcggt
    26641 gcaggagatc gcacacctga ctgaagaggt tgccccgtcc actacgatgc aggagcagct
    26701 ccgcccgatg ctgcacctga ttctcgacaa cgaaagcaag gtaaagcagt tattacagat
    26761 tcggatggat gaactggcga aactggtcgg tcagtcatcg gtgcaaaaat cggtgttaag
    26821 tgcctatggc gatcagggcg gctttgtgct ggctccgcag gataacctct tttgaatctt
    26881 aatgagccga tggctcgcga ataatccgat tacggctacg cttctaatat tccccttgaa
    26941 tggagtcgaa gaatgcgtaa tcccacgctg ttacaatgtt ttcactggta ttacccggaa
    27001 ggcggtaagc tctggcctga actggccgag cgcgcagacg gttttaatga tattggtatc
    27061 aatatggtct ggttgccgcc cgcctataaa ggcgcatcgg gcggctattc ggtcggctac
    27121 gactcctatg atttatttga tctaggtgag tttgatcaga aaggcagcat ccctactaaa
    27181 tatggtgata aagcacaact gctggccgcc attgacgccc tgaaacgtaa tgacattgcg
    27241 gtgctgctgg acgtggtagt caaccacaaa atgggcgcgg atgaaaaaga agctattcgc
    27301 gtgcagcgtg taaatgctga tgaccgtacg caaattgacg aagaaatcat tgagtgtgaa
    27361 ggctggacgc gttacacctt ccccgctcgt gccgggcaat actcgcagtt tatctgggat
    27421 ttcaaatgtt ttagcggtat cgaccatatc gaacaccctg atgaagatgg catttttaaa
    27481 attgttaacg actataccgg agaaggctgg aacgatcagg ttgatgatga actcggtaat
    27541 ttcgattatc tgatgggtga gaatatcgat tttcgcaatc atgccgtgac ggaagagatt
    27601 aaatactggg cgcgctgggt gatggaacaa acgcaatgcg acggctttcg tcttgatgcg
    27661 gtcaaacata ttccggcatg gttttataaa gagtggatcg aacacgtaca ggaagttgcg
    27721 ccaaagccgc tgtttattgt ggctgaatac tggtcgcatg aagtcgataa actgcaaacg
    27781 tatattgatc aggtggaagg caaaaccatg ctgtttgatg cgccgctgca gatgaaattc
    27841 catgaagcat cgcgcatggg tcgcgactac gacatgacgc agatcttcac tgggacgctg
    27901 gtggaagccg atccttttca cgccgtaacc ctcgttgcca accacgacac ccaaccgttg
    27961 caagccctcg aagcaccggt cgaaccgtgg tttaaaccgc tggcgtatgc cttaattttg
    28021 ttgcgggaaa atggcgttcc ttcggtgttc tatccggacc tctacggtgc acattacgaa
    28081 gatgtcggtg gtgacgggca aacctatccg atagatatgc caataatcga acagcttgat
    28141 gagttaattc tcgcccgtca gcgtttcgcc cacggtgtac agacattatt tttcgaccat
    28201 ccgaactgca ttgcctttag ccgcagtggc accgacgaat atcccggctg cgtggtggtc
    28261 atgtcgaacg gagatgatgg cgaaaaaacc attcatctgg gagagaatta cggcaataaa
    28321 acctggcgtg attttctggg gaaccggcaa gagagcgtag tgacagacga aaacggcgaa
    28381 gcgaccttct tttgcaacgg cggcagcgtc agcgtgtggg ttatcgaaga ggtgatttaa
    28441 atccatcccc ggcggcaagc cggggagatt tcattacggc agtggcgtcg gtaacggcgc
    28501 tttatccagc gcggcggcac actctgccgt cgggcgatcc acacgctcca tcgtcatacc
    28561 atcgtactca atggtgttac cgtcacgctc aacctcgtac agctcacgtt ttacggtaac
    28621 gttagtgaga tcatcagaca tcaatgtcaa cttacccggc acggcaatcc cccgctgcca
    28681 ctgacggcaa tccaaggtat cgccctcttt agtgacaatc aggctgccta tcgcttccgg
    28741 actgaccagc gcacgttgcg gccctttggt ctgccagtag cccgccagcc agtccggcgc
    28801 gggggttttc acgacattgt tatagttttc gacttcggca cagcccgcca gcaacatgag
    28861 cgcgcctgca attgcgagtt ttttcatcat tcgtcctgca tgagaagaaa agaggattgt
    28921 ggcattaaag ctctgatgtc gccagccttt ccaggttcag agagaaattt gatctaacta
    28981 ttatttttgt gaattattac cccgaaaata aatttgcatg gtgtatgatt cgcggctctt
    29041 gtttcaaacc ttctgagttc agaggctaca ttcatgtcat ggcagcaatt caaacacgcc
    29101 tggttgatta aattctgggc acccatccct gcggtcatcg cggcgggtat tctctccact
    29161 tactattttg gcattactgg caccttttgg gctgtcacgg gtgaatttac ccgttggggc
    29221 ggtcagctcc tgcagctgtt tggcgtccat gctgaagagt ggggttattt taaaattatc
    29281 catctggaag gatcgccatt aacccgcatc gacggcatga tgatcctcgg tatgtttggc
    29341 ggctgctttg ctgctgcgct gtgggccaac aatgtcaaac tacgcatgcc acgcagccgt
    29401 atccgcatta tgcaggccat cattggcggc attatcgccg gttttggcgc gcgtctggca
    29461 atgggctgta acctggcggc gttctttacc ggcattcctc agttctcgct gcatgcctgg
    29521 ttctttgcca tcgccactgc cattggttca tggtttggcg cgcgctttac cctgctgccc
    29581 atcttccgta ttcccgtgaa aatgcagaaa gtttctgccg cctcaccgct gacgcaaaaa
    29641 ccggatcagg cgcggcgtcg ttttcgtctc gggatgctgg tctttttcgg catgctgggc
    29701 tgggcgctgc tcacggcgat gaaccaacca aaactggggc tggcaatgct gtttggcgtc
    29761 ggctttggtt tactgattga acgtgcgcaa atctgcttta cttcggcgtt ccgcgatatg
    29821 tggatcaccg gacgtaccca tatggcgaaa gcaatcatta tcggtatggc ggtgagtgcc
    29881 atcgggatct tcagttacgt acagttaggc gttgaaccta aaatcatgtg ggcgggacca
    29941 aatgcggtaa ttggtggttt actttttggt tttggcatcg tgctggcagg cggctgcgaa
    30001 accggctgga tgtaccgcgc ggtagaaggc caggtgcact actggtgggt cggtctgggc
    30061 aacgtgatcg gctcaacgat tctggcgtat tactgggatg atttcgctcc ggcgctggcc
    30121 accgactggg acaaaatcaa cctgctgaaa acttttggtc cgatgggagg cctgctggtg
    30181 acatatttgc tgctgtttgc tgcgctgatg ttgattatcg gctgggaaaa acgcttcttc
    30241 ccccgtgagg caccgcagac tgctaaggag atcgcatgaa aaatatcgtt cctgattacc
    30301 gccttgatat ggtgggtgaa ccctgccctt atccggcagt cgcaaccctt gaggcgatgc
    30361 cgcaattaaa aaaaggtgag atcctggaag tggtgagcga ctgtccgcag tcgatcaata
    30421 atattccact ggatgcgcgt aatcacggct acacggtgct ggatattcag caagacgggc
    30481 cgaccattcg ttatttaatt cagaagtaat ccctcattcc cgctgtggcc cacagcggga
    30541 acatcgttac ctctgacaca tagattttac ccgccccctc tcctattctt ttcagtatga
    30601 tctcaggagg ttgctatgtg tggacgcttt gcccaatccc aaacgcgtga ggattacctt
    30661 gcgcttctcg cggaagatat tgaacgcgat attccctacg atcccgaacc cattggcaga
    30721 tacaatgtcg cgccgggaac caaagttctt ctgctcagtg aacgtgatga acaccttcat
    30781 ctggatccgg ttttctgggg atatgctccc ggatggtggg ataaaccgcc gctgattaac
    30841 gcccgcgtag aaactgcctc aaccagtcgc atgtttaaac cgctctggca acatggtcgg
    30901 gcaatctgtt ttgccgatgg ctggtttgag tggaaaaaag aaggcgacaa aaaaagcctt
    30961 attttatcta tcgcgctgac ggacaacctg tttttatggc cgcgataggt agcataccgt
    31021 ttgagcgtgg tgacgaagcc gaaggatttt tgatcgtcac tgcggcggca gatcaaggtc
    31081 tggtagatat tcatgaccga cgcccgctgg ttctgtcgcc agaagctgca cgggaatgga
    31141 tgcggcaaga cattggtggt aaagaagcct cagaaatcgc taccagaagc tgtgtgccag
    31201 caaaccagtt cacctggcac cctgtgtcgc gcacggtggg taatgtcaaa aaccagggcg
    31261 cggagttaat tcaacctgtt tgttgacatc ctccacgccc tgaatgacga ggacccctgc
    31321 tacgttcagg ctgtcgcctg aatcatttcg gtgggttcct gcttcaacga gcggcctgac
    31381 tgcaccatct ctccacaggc aagaacggcg tgccccgccg ctaaaatgtt acgagctcca
    31441 tttacatcgg cgttcgctgt atatccacat acctggcatc tgaatttact ttgtgacagg
    31501 cgattttctt tcgctgtatg accacagcac gcgcaacgct ggcttgtgta cgctggcgga
    31561 acagcaagca cctgaccgcc acgccagagc tgcttgtact caagctggcg gcgcatttca
    31621 taccagccct gatccagtat cgaacggttt aaacctgatt ttgcccggac attgcgcccc
    31681 ggctggctga ccgtacccgc tgctgacttt gacatgttgc tgaccttcaa atcctcaatg
    31741 actatcatta cgtggttttt gctgacggtc gttgtgactt tgtgaaggta gtccctgcgg
    31801 atatttgcga tacaggaatg cagtcgctgt attttgcgtt tctgcttctg ccagttgttg
    31861 ctgaatttga ccttgcggct taactgtcgc tgaagtctcg ccagcttctt ctggtttttc
    31921 tgaaaactgt ttacaggctc aaagactgtg ccatctgaca gcgaggcaag tttagccacg
    31981 ccagcatcca gcccgaccat tgatgctgaa gggtgaaccg gagttgatac ttcactttct
    32041 gcctgaatac tgatgtacca cttaccgcag gactggctga cagtgacatt tttcacaaca
    32101 cccgtgacct gacggctgtt ccggtagcgc atccagccaa gttttggcag aaaaatacgg
    32161 ctgttttcct gagcgagctt aacgccctgc ggatagcgga atgcatcatt ctgtcaccgc
    32221 tttttgaatc aggggaacgc cgagcgcttc tggaaaaagt ttttgcaggc ctgctcaggg
    32281 tctttcagtg actgttgcaa tggctgtgac ggagcatctt taatccattg cgtttcagtg
    32341 gcatttttcc actcaaccaa tcaggaagcc attttcgcgt aagggatgtc tttgttaccg
    32401 gccttatgat tctcattctg acgtgccaga gtacgactga aaacgaaacg acatgcgcct
    32461 gcgaagcgcc tcatttcacg cgcctgttga ccaccgggtc ttaaccggaa tttgaatacc
    32521 tgtagtcgtt tcacagggca aattatactt tggtctgtga aaaaaactga tatccgacgc
    32581 ggaaggcact gtgttttcct gatgcatgct caccgggtct ttgttaccaa gtaccgacgc
    32641 cagatttttg accatgacgc gacagaaaaa ctacgcactt acttttcaaa tgtatgtgct
    32701 tattttgaag ctgaactggt tgaaatggat ggcgaaccag atcacgtcca tctgttaatt
    32761 aactatcccc ccaaactggc gatatccagt ctggtaaaca aacagcctca aaggcgtatc
    32821 gggtaggtta ctgcgacggg atcgaccaga tattgctgcc aggtattacc gcaaagatgt
    32881 tttgtggcgt cccggctatt ttgccagtag ctgcggaggt gcgccaatat ccgtcatccg
    32941 ccaatacatt gaacaacagc aaacacctgg tcagatgaaa aaacgagcct tatatccccg
    33001 ccctgaagga cgggctttta cggtgcactg gataagcctg gagattatct acacctgcat
    33061 ggacatcatt gtactatgcc ccgtacatgc cccatcacat cctcaacatc aaagtattag
    33121 agccttgcaa accattatgc gtctcagttt gtctcatcta tcttacgcaa aaaaaataga
    33181 actaatatca agcggcgcta tattgcgtat taaataaaac cacctcaatt acattaataa
    33241 atctaaataa atatcatgca aataatttcc atggttattt ttaggctatt taaattaaaa
    33301 aaataaatta tgagtcaatc ataaataaca acctttaata taatttgact accaaattta
    33361 tattcgttca caaaatagtc atgcattgtg tgcaatagaa acagttcaga taaaaatagg
    33421 gatctacttc acaatttaaa tgagaaacta aaacttacat cttgaaataa tctcattgat
    33481 tggatgaata tttgtagtgc agtgacatca tttttaaata atagttcaaa taaaagggct
    33541 cacgatgaaa aaattaacag tggcaatttc tgctgtagct gcatcagtac tgatggcgat
    33601 gtctgctcag gcagcagaga tttacaataa agacagtaac aagctggatc tgtacggaaa
    33661 agttaatgct aagcactact tctcttctaa cgatgcagat gatggcgaca caacttatgt
    33721 tcgtctgggc ttcaaaggcg aaactcaaat caacgatcaa ctgactggtt tcggtcagtg
    33781 ggaatatgaa ttcaaaggca accgtgctga atctcaaggt tcttccaaag ataaaacccg
    33841 tcttgccttc gctggcctga aattcggtga ctacggctcc atcgattatg gccgtaacta
    33901 cggtgtagca tacgatatcg gtgcatggac cgacgttctg ccagaattcg gtggcgatac
    33961 ctggacccaa acagatgtgt tcatgactgg tcgcaccaca ggtgttgcaa cctatcgtaa
    34021 caacgacttc tttggtctgg ttgatggtct gaactttgct gctcagtatc agggtaaaaa
    34081 tgaccgcact gacgtaactg aagctaacgg tgatggtttc ggtttctcca ctacttatga
    34141 gtatgaagga ttcggtgtag gtgcaaccta tgctaaatct gaccgcacta atgatcaggt
    34201 tatctacggt aacaacagcc tgaatgcatc tggtcaaaat gctgaagtat gggcagctgg
    34261 tctgaaatat gatgcgaaca acatctatct ggctaccacc tattctgaaa cccagaacat
    34321 gactgttttt ggtaataacc atattgccaa caaagcacaa aacttcgaag tagttgcaca
    34381 atatcagttc gacttcggtc tgcgtccgtc cgttgcttac ctgcaatcta aaggaaaaga
    34441 cttgggtgcg tggggtgatc aggacctgat tgaatatatt gatgtaggtg caacctatta
    34501 cttcaacaaa aatatgtcca cttttgttga ttacaaaatc aacctgattg ataagagcga
    34561 tttcacgaaa gcatctggcg ttgctaccga tgatatcgtt gctgtaggat tggtttacca
    34621 gttctaatct gattactcaa agatatgttg tgggaggctt tgcctcccca acatataagt
    34681 ggcgctctca agccactttc cttagaagca ctatcttgct tctgactata taaattaccc
    34741 tttattttct gggacgtttc aacgccccat ttttaataat actcagtgaa caattaacgt
    34801 atcaattaga tttaataaca acgatatccg tacctaacct gatatctaat gtcataagca
    34861 tccctttaat tatcccctca acctcttgta aatttcccac tcttgagctc cacatagccc
    34921 aaacgccgga tgcaaaaaaa atcacacgtg catctctgaa attgtgatgg aggaagtgag
    34981 tatcaattat atgtgcttat aaacgttccc gattggcaca gaagtcaccg gattgttcag
    35041 gctccaatag gttgagtatg tcgtatttat tttccaaaat aaaaatcgat gctgttgctc
    35101 cttaataaaa tacttttact ctctggacgc ttttcttagc tctcttggtg ttatattaaa
    35161 acgattatgg aacctttcag taaaacgaga aggacacttg taaccatttt ccctggcaat
    35221 ctcgctgatg ggttttactg tcgtttgtat agcaggacaa cgcattattt aacctcacgt
    35281 cgtccaatat actttgaaaa cttattccct cgcttgccag acggcgatgc aatgtggaaa
    35341 cagaaatgta gagatatctc gctaccttgt ttgctgtcca ttttgttcta ggttcggata
    35401 gcattaggtt ataacaacga cttattaatg attgtttact atctgataaa agtaaacaat
    35461 taacatgatg tgctcctaat gaaagtagaa cgcccattgc taagtgttcc tgaatttgcg
    35521 ttgagaagcc tcgggaaata gatgttttta gttgctccca acaatatatt aactcggggt
    35581 tctgaggtaa gcagaaacct gtcgtgttac gaatttgatc agttatcgca taaaattttt
    35641 gaaacttttc aattagatca atgggataat aaagcatttc tgcaagataa agacctgctt
    35701 ctggataatt cacaatataa aattcatatc cacagggaaa taatattatc tgattattat
    35761 caacagttag actatgcgtc tcccaattga taactttttt tccctgacgg atacgacata
    35821 aagctggcat aagaggctta accctgtgaa tctcatgatg tttatgcatc cgtatttctt
    35881 caatctttag gttagactta tctcttgcca gcatactctt accctatttt atctcataaa
    35941 ctggtgttat ctcagcggtt gcaattttat tggcattaag catataacca actaacgctc
    36001 cgctggaatt agaatccaca ggaatcttat cagtttttag agcccatact ttaaactggt
    36061 aatgatgggg tttatctcct ttgggaggac atgctccacc aaatcctgca taaccaaaat
    36121 catttcggcc ttgaacagca ccagtcggaa gttttgtacc atcacgtctt cctgcatcag
    36181 tgggcaaata tgttacagtt gctggaatat tagcaacagt ccagtgccac catccactgc
    36241 ctgtaggagc atctggatca tacacagtta cggcaaagct tttggtgcct tcaggagcac
    36301 cagaccaggt taatgagggc gatgtattac caccttcaca cccaaatcca gaaaatacat
    36361 gagacgttgt aagttgctct cctgttttta tttcattact agtgacctga aatgctgcgg
    36421 cctgcgcaga aaatgttatg aatgccaata cacttgaaac gataagtttt ttcataaaaa
    36481 cctcattgtt gtgacctatc gttattttat ttgatgtgcc ttgatctcat tatgaataaa
    36541 ggcgcaatga tcgcgcaaat gcaatcataa ttataaaggt gccccgaaca ttagccataa
    36601 tatgcacaaa tcagattcct gttatctcaa ggagaattat tttttaatgc ggtgcacttc
    36661 gtgacaacaa gttaatgact aacacaccgg cacaaatcaa catcatgcct atgatagctg
    36721 gcaggtccag tcgttgacca aaaaatcccc aggaaagcaa actaatcaga acgataccga
    36781 ctcctgacca gatagcataa gcaatccctg taggtatata agccagcgtc tgagctaata
    36841 accagaatga tgcacaatag caaataattg tgccaacaga gggccataac cgtgtaaacc
    36901 cctctgaaaa cttcattaag gttgtaccaa tgacctctgc aagtattgcg ccaccaagat
    36961 aaatataagg gttcatagca tattctttcc tgttcatact ggagagaatt gtactacagt
    37021 ttgaacacaa ctcaccgttt tcatcctggt gtatgtgtat ccaaggggct ctggcgtggc
    37081 aagagataaa ggcaggaaca ataatgatca gaaggaaccc caggctaacg aatgagttca
    37141 ctcttcctgg gagcaaagcg aagacaaaac atgttatcgc gtttacggat cgtggatggc
    37201 agaaaacaac caggatcagg tatccatttt aaaccagaaa ttgagtgagt ttgccccatc
    37261 catgccccac gctgtgggat cggatataat taaacaagca tagtcaacag gttacacaac
    37321 ctacacctgc atggacatca cttcctgata cgccgccacc agcttattac gcacctgaat
    37381 ccccatttgc atagaaactg aggctttttg catatcggtc atcacatcgt ttagcgccac
    37441 gccgggttca ccgagggtga atttttctgc ctgcgtgcgg gcgactgttt gtgtatcgct
    37501 tatgcgatcg agcgcggcgt gcagctgccc ggcaaaacta atggtcggtt gcggcagtga
    37561 ttcctgcgca cacgcactca tcgccgtagc ctgtaactgg ctgataaccc cttcaatccc
    37621 ctgtatcgct gacattttcg tctcccggat aatttctggt aacaaagcct accagtaagt
    37681 caataagaca aaggcgctaa atagcaacaa aaaaactggt ttattggcgg atagaaaaaa
    37741 cgaaagcaca aataatggga gcgtcaattt ttcgagtttg ctgacccgag agtgagtctt
    37801 gttccacttt gccaataacg ccgtccataa tcagccacga ggtgcgcgat gaatgcgact
    37861 gcagcccaga caaaatctct tgagtggctt aatcgcctgc gtgcgaatcc gaaaattcca
    37921 ttgattgtta ccggttccgc ggcagtggcg gtcatggtcg cactgatcct gtgggcgaaa
    37981 gcccccgact accgcacatt attcagcaat ctttccgatc aggatggtgg cgcaattgtc
    38041 agccaactga cgcaaatgaa tattccttac cgcttcagcg aagccagcgg cgctattgag
    38101 gttccggcag ataaagttca cgaactgcgc ctgcgcctgg cgcaacaagg tttgcccaaa
    38161 ggcggcgcgg tcggtttcga actgctcgat caggaaaagt ttggtatcag ccagttcagc
    38221 gaacaggtga attatcagcg ggcgctggaa ggcgagcttt ctcgtaccat cgaaactatc
    38281 ggcccggtaa aaggggcgcg cgtacatctg gcaatgccga aaccgtcttt attcgtccgt
    38341 gaacaaaaat ccccttctgc atcggtgacg gtaaatctgt tacccggccg cgcactcgat
    38401 gaagggcaaa ttagcgctat tgtacatctg gtttccagcg cggttgctgg tctgccgcct
    38461 ggaaacgtca cgctggtgga tcagggcggg catctgttaa cccagtccaa taccagcggg
    38521 cgcgatctta atgacgctca gttgaaatat gccagcgatg tcgaaggccg tattcagcag
    38581 cgtattgaag cgatcctgtc acctattgtt ggtaacggta atattcacgc ctaggtcacg
    38641 gcgcaactgg acttcgccag taaagaacaa acggaagaac agtatcgccc taacggtgat
    38701 gaatctcatg cggcgcttcg ttcacgccag cttaatgaga gcgagcaaag cggttccggt
    38761 tatccgggcg gcgtaccggg ggcgttgtcg aatcaaccgg cacctgcgaa taacgcgcca
    38821 atcagcacgc ctccgacaaa tcaaaataac cgccagcagc aggcgagcac caccagcaat
    38881 agtgggccgc gtagcacaca gcggaatgaa accagtaact acgaagtcga tcgcaccatt
    38941 cgtcatacca aaatgaacgt gggcgatgtg caacgtctgt cagtcgcggt agtggtgaat
    39001 tacaaaacct tgccagacgg taaaccattg cctctcagca acgaacagat gaagcaaatt
    39061 gaagctctga cccgcgaggc gatgggcttt tctgaaaaac gcggtgattc gctcaatgtt
    39121 gttaactcgc cgttcaatag cagtgacgaa agcggcggag cactgccgtt ttggcaacag
    39181 caagtgttta tcgatcagtt gctggctgcc ggtcgctggt tgctggttgc tggtactgct
    39241 ggtggcgtgg ttgctgtggc ggaaagcggt acgcccgcag ctaacacgtc gcgccgaggc
    39301 ggtgaagact gtgcagcaac aggcgcaggc ccacgaggaa gtggaagatg cggtggaagt
    39361 ccgtctgagc aaagacgaac aactccaaca acggcgcgct aaccaacgac tgggggcaga
    39421 agtcatgagc cagcgtatcc gtgaaatgtc tgataacgat ccgcgcgtgg tggcgctggt
    39481 cattcgccag tggataaata acgatcatga gtaacctgac aggcaccgat aaaagcgtca
    39541 tcctgctgat gaccattggc gaagaccggg cggcagaggt gttcaagcac ctctcccagc
    39601 gcgaagtgca aaccctgagc gctgcaatgg cgaacgtcac gcagatctcc aacaagcagc
    39661 taaccgatgt gctggcggag tttgagcaag aagctgaaca gtttgctgca ctgaatatca
    39721 acgccaacga ttatctgcgt tcggtattgg tcaaagctct gggtgaagaa cgtgccgcca
    39781 gcctgctgga agatattctc gaaacccgcg ataccgccag cggtattgaa acgctcaact
    39841 ttatggagcc gcagagcgcc gccgatctga ttcgcgatga gcatccgcaa attatcgcca
    39901 ccattctggt gcatctgaag cgcgcccagg ccgccgatat tctggcgttg ttcgatgaac
    39961 gtctgcgcca cgacgtgatg ttgcgtatcg ccacctttgg cggcgtgcag ccagccgcgc
    40021 tggccgaact gaccgaagta ctgaatggct tgctcgacgg tcagaatctc aagcgcagca
    40081 aaatgggcgg cgtgagaacg gcagccgaaa ttatcaacct gatgaaaacg cagcaggaag
    40141 aagccgttat taccgccgtg cgtgaattcg acggcgagct ggcgcagaaa atcatcgacg
    40201 agatgttcct gttcgagaat ctggtggatg tcgatgatcg cagcattcag cgtctgttgc
    40261 aggaagtgga ttccgaatcg ctgctgatcg cgctgaaagg ggccgagcag ccactgcgcg
    40321 agaaattcct gcgcaatatg tcgcagcgtg ccgccgatat tctgcgcgac gatctcgcca
    40381 accgtggtcc ggtgcgtctg tcgcaggtcg aaaacgaaca gaaagcgatt ctgctgattg
    40441 tgcgccgtct tgccgaaact ggcgagatgg tgattggcag cggcgaggat acctatgtct
    40501 gataatctgc cgtggaaaac ctggacgccg gacgatctcg cgccaccacc ggcagagttt
    40561 gtgccaatgg tcgagtcgga agaaaccatt attgaagagg ccgaacccag ccttgagcag
    40621 caactggcgc aactgcaaat gcaggcccac gagcagggtt atcaggcggg gattgccgaa
    40681 ggtcgccagc aaggtcacga acagggctat caggaaggac tggcccaggg gctggaacaa
    40741 ggtctggcag aggcgaaggc tcaacaagcg ccaattcatg cccggatgca gcaactggtc
    40801 agcgaatttc aaactaccct tgatgcgctt gatagtgtga tagcttcgcg cctgatgcag
    40861 atggcgctgg aggcagcacg tcaggtgatc ggtcagacac caacggtgga taactcggca
    40921 ctgatcaaac agatccaaca gttgttgcag caagaaccgt tattcagcgg gaaaccacag
    40981 ctgcgcgtac acccggatga tctgcaacgt gtggatgata tgctcggcgc aactttaagt
    41041 ttgcatggct ggcgcttgcg gggcgatccc accctccacc ctggcggatg taaagtctcc
    41101 gccgatgaag gcgatctcga cgccagtgtc gccactcgct ggcaagaact ctgccgtctg
    41161 gcagcaccag gagtggtgta atgaccacgc gcctgactcg ctggctaacc acgctggata
    41221 actttgaagc caaaatggcg cagttgcctg cggtacgtcg ctacgggcga ttaacccgcg
    41281 ctaccgggct ggtgctggaa gccaccggat tacaattgcc gctcggcgca acctgtgtca
    41341 ttgagcgcca gaacggcagc gaaacgcacg aagtagaaag cgaagtcgtt ggctttaacg
    41401 gtcaacggct gtttttaatg ccgctggagg aagtggaagg tgtcctgccc ggcgcgcgtg
    41461 tatatgccaa aaacatttcg gcagaagggc tgcaaagcgg caagcagttg ccgctcggtc
    41521 cggcgttatt aggtcgtgtt ctggacggta gcggtaaacc gctcgatggc ctgcccttcc
    41581 ccgatacgac ggaaaccggt gcgctgatta ccccgccatt taacccgttg caacgtacac
    41641 cgattgaaca tgtgctggac accggcgtgc gcccaatcaa tgccctgctt accgtcggac
    41701 gcgggcagcg tatggggctg tttgccgggt cgggcgttgg taaaagtgtg ctgctgggga
    41761 tgatggcacg ctacacccgc gccgatgtca ttgtcgtggg tttgattggt gaacgtgggc
    41821 gcgaagtaaa agattttatt gagaatatcc tcggtgccga aggacgtgca cgctcagtgg
    41881 tgattgccgc tccggcggat gtttctccgc tcctgcgaat gcaaggtgcc gcctatgcca
    41941 cgcgcattgc cgaagatttt cgcgatcgtg gtcagcatgt gttgctgatt atggactccc
    42001 tcacccgcta cgcgatggcc cagcgtgaaa ttgcgctggc gattggcgaa ccacctgcca
    42061 ctaaaggtta tccaccgtcg gtgtttgcca aattaccggc actggtcgag cgtgccggta
    42121 atggcattag cggcggcggc tcgattaccg cgttttatac cgtgctcacc gaaggcgatg
    42181 accagcagga ccccattgct gactccgcgc gggcaatcct cgacggtcac attgtgctgt
    42241 ctcgccgact ggcggaagcc gggcactatc cggctatcga tattgaagcg tcgatcagtc
    42301 gcgcaatgac ggcgttgatc agtgagcaac attacgcgcg agtgcgcacc ttcaaacagc
    42361 tgttgtcgag ttttcagcgt aaccgcgatc tggttagcgt cggcgcgtat gccaaaggca
    42421 gcgatccgat gctcgataaa gccatcgccc tgtggccgca gctggagggc tatttgcaac
    42481 aaggcatttt tgaacgcgcg gactggaaag cgtctctcca ggggctggag cgtattttcc
    42541 cgacagtgtc ataacccagg agataacggc agatggcgga agaacagctc aaaatgttga
    42601 tcgattatca gaatgaatat cgcaataacc tcaatagcga tatgagtgcc gggatgacca
    42661 gcaaccgctg gatcaactat cagcagttta tccagacgct ggaaaaagcc attactcagc
    42721 atcgccagca acttaatcag tggacgcaga aagttgacat tgccctgaac agctggcgag
    42781 aaaagaaaca acgtttgcag gcctggcaga cactgcagga acggcaatcc acggcggcac
    42841 tgcttgcaga aaaccgcctc gatcagaaaa agatggatga gttcgcccag cgcgccgcca
    42901 tgaggaaacc tgaatgattc gcttagcgcc cttgattacc gccgacgttg acaccaccac
    42961 attgcctggc ggcaaagcca gcgatgctgc acaagatttt ctcacgttgt tgagcgaagc
    43021 attagcaggc gagacaacaa ctgacaaagc ggccccccag ttgctggtag caacagataa
    43081 gcccacgaca aaaggcgagc cgctggtcag cgatattctt gccgatgcgc aacaagcgga
    43141 tttactgatc cctgtggatg aaacacttcc tgtcatcaac gacgaacaat ccacatcaac
    43201 accgttaacc accgctcaga cgatgacgtt ggctgcggtg gctgacaaaa atacgacaaa
    43261 agacgaaaaa gcggatgatc ttaatgaaga cgtcaccgca agtctgagcg ccctttttgc
    43321 gatgttgccg ggttttgaca atacgcccaa agtgactgat gcaccgtcaa ccgtgttacc
    43381 ggcagagaaa ccaacgctct tcacaaaact gacttctgcg caactcacta cagcacagcc
    43441 tgatgacgcc cccggcacac cagctcagcc attaacaccg ctggtagcag aagcccagag
    43501 taaagcggaa gtcatcagca cgccttcgcc ggtgaccgct gacgccagcc cgctaatcac
    43561 tccacaccag acacagccac tgcccaccgt cgccgcgcct gttttgagtg caccgctggg
    43621 ttctcacgaa tggcaacaat cattgagcca gcatatttcg ctgttcaccc gccaggggca
    43681 acaaagtgca gagttgcgtc tgcacccaca ggatttaggt gaagtgcaaa tttccctcaa
    43741 agtggatgat aaccaggcgc agatccagat gatttcaccg catcagcacg tacgcgccgc
    43801 cctggaagca gcgctgccgg tactgcgtac gcagctggcc gaaagtggca ttcagttagg
    43861 gcaaagcaac attagtggcg aaagctttag tggtcagcag caggccgctt cccagcaaca
    43921 gcaaagccaa cgcacagcaa accatgaacc tctggcgggg gaagacgacg atacgcttcc
    43981 ggttcccgtc tctttacaag ggcgtgtaac aggtaacagc ggcgttgata ttttcgccta
    44041 acgtcagagg tagcaccgta atccgcgtct tttccccgct ttgttgcgct caagacgcag
    44101 gataattagc cgataagcag tagcgacaca ggaagaccgc aacacatgac tgattacgcg
    44161 ataagcaaga aaagcaagcg atcgctttgg atcccgattc tggtattcat taccctcgcg
    44221 gcctgtgcca gcgcaggtta cagctactgg cattcgcatc aggttgccgc tgacgacaaa
    44281 gcgcagcaac gcgtcgtgcc ctccccggtc ttctatgcgc tggatacctt tacggtcaat
    44341 ttgggcgatg cggatcgcgt gctttatatc ggcataaccc tgcgactgaa agatgaagca
    44401 acccgctcgc ggctgagtga gtatttgccg gaagtccgta gtcgcttgct gttactgttt
    44461 tcacgtcagg atgctgccgt actggcgaca gaagaaggca agaaaaacct gattgccgag
    44521 attaaaacca cactttccac cccgcttgtt gccgggcaac cgaaacagga tgtcaccgac
    44581 gtgctgtata ccgcttttat tctgcgataa cgacatgggc gatagtattc tttctcaagc
    44641 tgaaattgat gcgctgttga atggtgacag cgaagtcaaa gacgaaccga cagccagtgt
    44701 tagcggcgaa agtgacattc gtccgtacga tccgaatacc caacgacggg ttgtgcgcga
    44761 acgtttgcag gcgctggaaa tcattaatga gcgctttgcc cgccattttc gtatggggct
    44821 gttcaacctg ctgcgccgta gcccggacat taccgtcggg gccatccgca ttcagccgta
    44881 ccatgaattt gcccgcaacc tgccggtgcc gaccaacctg aacctaatcc acctgaaacc
    44941 gctacgcggc acagggctgg tagtgttctc gccgagtctg gtgtttatcg ccgtggataa
    45001 cctgtttggc ggcgatggac gcttcccgac caaagtggaa ggtcgcgagt ttacccatac
    45061 tgaacagcgc gtcatcaacc gtatgttgaa gctggcgctg gaaggctata gcgacgcctg
    45121 gaaagcgatt aatccgctgg aagttgagta cgtgcgttcg gaaatgcagg tggaatttac
    45181 caatatcacc acctcgccga acgacattgt ggttaacacg ccgttccatg tggagattgg
    45241 caacctgacc ggcgaattta atatctgcct gccattcagc atgatcgagc cgctacggga
    45301 attgttggtt aacccgccgc tggaaaactc gcgtaatgaa gatcagaact ggcgcgataa
    45361 cctggtgcgc caggtgcagc attcacagct ggaactggtg gccaactttg ccgatatctc
    45421 gctacgcctg tcgcagattt taaaactgaa acccggcgac gtcctgccga tagaaaaacc
    45481 cgatcgcatc atcgcccatg ttgacggcgt cccggttctg accagtcagt acggtactct
    45541 caacggtcag tatgcgttac ggatagaaca tttgattaac ccgattttaa attctctgaa
    45601 cgaggaacag cccaaatgag tgacatgaat aatccggccg atgacaacaa cggcgcaatg
    45661 gacgatctgt gggctgaagc gttgagcgag caaaaatcaa ccagcgaaaa aagcgctgcc
    45721 gacgcggtgt tccagcaatt tggcggtggt gatgtcagcg gaacgttgca ggatatcgac
    45781 ctgattatgg atattccggt caagctgacc gtcgagctgg gccgtacgcg gatgaccatc
    45841 aaagagctgt tgcgtctgac gcaagggtca gtcgtggcgc tggacggtct ggcgggcgaa
    45901 ccgctggata ttctgatcaa cggttattta atcgcccagg gcgaagtggt ggttgttgcc
    45961 gataaatatg gcgtgcggat caccgatatc attaccccgt ccgagcggat gcgccgcctg
    46021 agccgttagt gatgaataac catgctactg tgcaatcttc cgcgccggtt tctgctgagc
    46081 cactgctgca ggtgagcggc gcactcatcg ccattattgc cctgatcctc gctgctgcct
    46141 ggctggtaaa acggctggga tttgccccta aacgcactgg cgttaacggt ctgaaaatta
    46201 gcgccagtgc ttcgctgggc gcgcgtgaac gggtcgtggt ggtcgatgtg gaagatgcac
    46261 ggctggtgct cggcgttacc gcagggcaaa tcaatctgct gcataaactt cccccttcag
    46321 caccaacgga agagataccg cagaccgatt ttcagtcggt catgaaaaat ttgcttaagc
    46381 gtggcgggag atcctgatgc gtcgtttgtt ttctgtcgca ccggtccttc tctggctgat
    46441 tacgcccctc gccttcgcgc aactgccggg tatcaccagc cagccgctac ctggcggtgg
    46501 acaaagctgg tcgctcccgg tgcagacgct ggtgttcatc acctcgttga cgttcattcc
    46561 agcgatttta ctgatgatga ccagtttcac ccgcatcatt attgtttttg gtttattgcg
    46621 taacgcgctg ggaacgccct ccgcgccacc taaccaggta ttgctggggc tggcactgtt
    46681 tttgaccttt tttattatgt cgccagtgat caacaaaatt tatgtcgatg cgtaccagcc
    46741 attcagcgaa gagaaaatct caatgcagga ggcgctggaa aaaggggcgc agccgctgcg
    46801 tgagtttatg ctgcgtcaga cccgcgaggc agatttaggg ttgtttgcca gactggcgaa
    46861 taccggcccg ttgcaggggc ctgaagctgt gccgatgcgc attttgctcc cggcctacgt
    46921 gaccagcgag ttgaaaaccg catttcagat aggcttcacg attttcatcc cttttttgat
    46981 tatcgacctg gtgattgcca gcgtgttgat ggcattgggg atgatgatgg ttccccccgc
    47041 caccattgct ctgcccttta aactgatgct gtttgtactg gtggatggct ggcaattgct
    47101 ggtcggttcg ctggcgcaga gcttttacag ctagagaggc aaaatgacac ctgaatcggt
    47161 catgatgatg ggaactgaag cgatgaaagt cgcgctggca ctggcagccc cgctgttgct
    47221 ggtagcgctg gtcacgggcc ttatcatcag tattttgcag gcagccacgc agattaacga
    47281 aatgacgctg tcgtttattc cgaaaatcat cgccgtattt atcgccatca ttattgccgg
    47341 accgtggatg ctcaatctgt tgctggatta cgtccgcacc ttgttcacta acctgccgta
    47401 tatcatcggg tagcccgtac aatgatgcag gagacaagcg atcaatggct ttcctggtta
    47461 agcctgtact tctggccgtt actgcgcgtg ctggcgctca tttctaccgc gccaattctg
    47521 agcgaacgca gcgtgccgaa acgggtaaaa ctgggtctgg caatgatgat cacgttcgcc
    47581 attgccccat cattacctgc caacgatgtt cctgtttttt cgttctttgc tctgtggctg
    47641 gccgtacagc agatcctgat cggcattgcg cttggtttta ccatgcaatt tgcctttgcc
    47701 gctgtgcgaa ccgctggcga aattatcggt ctgcaaatgg ggctgtcatt tgcgacattt
    47761 gtcgatcctg gcagccatct caatatgccc gttttagcgc gcatcatgga tatgctggca
    47821 ttactgctgt tcctgacatt taacggtcat ttatggttga tttcactgct ggtcgatacc
    47881 tttcacaccc tgccgattgg tagcgaaccg ttgaacagca atgcgtttct ggcacccacc
    47941 aaagcaggga gtttgatttt ccttaacggg ctgatgctgg cgttaccgct cattactctg
    48001 ttgctgaccc tgaatctggc attaggttta ctgaatcgta tggccccgca attatccatt
    48061 tttgttattg gatttccatt aactctgact gtcggcatct ctttaatggc ggcattaatg
    48121 ccgttaattg cgcctttttg cgaacattta ttcagtgaaa tttttaattt gctggctgat
    48181 attattagtg aattaccatt aatataattc cgtaacgttt atcatgctac cctaaggatt
    48241 atccgaaaaa taatatctgc gaacatcttc caggatactc ctgtagcgaa atatttgttt
    48301 taagctcact cacatatcgc aacatttact ttaagacaat tccaggcaaa ttatacaaca
    48361 ctttacggga tagtaagtcc gcctgaaaaa ttgcgagagt ggcgcattag gtgacccatg
    48421 ttgttccgtt tagtcatgat gaaatattca ggtaagggga attatcgtta cgcattgagt
    48481 gagggtatgc catgtcaacg attattatgg atttatgtag ttacacccga ctaggtttaa
    48541 ccgggtatct gttgagtaga ggggttaaaa aaagagaaat caacgacatt gaaaccgttg
    48601 atgaccttgc catagcttgt gattcacagc gtccttcagt ggtgtttatt aatgaggact
    48661 gtttcatcca cgatgcttct aacagtcagc gcatcaagca catcattaat caacatccca
    48721 atacgttatt tatcgttttt atggcaattg ccaatgttca ttttgatgaa tatctattgg
    48781 tcagaaaaaa tttattgatc agttctaaat cgattaaacc ggaatctctc gacgatatcc
    48841 ttggcaatat tctgaaaaaa gagacaacga taacctcgtt tttaaatatg ccgacgttat
    48901 cattgagccg aaccgaatcg agtatgttgc gaatgtggat ggcaggtcag ggaaccattc
    48961 aaatctccga ccaaatgaat atcaaagcca agaccgtttc atcgcataaa ggtaatatta
    49021 aacgtaagat caaaacgcat aataaacagg ttatctacca tgtcgtccga ctgacggata
    49081 atgtgactaa tggtattttt gtcaacatgc gctaacacat tctgactggt gatttcccac
    49141 cagtcaggct gaataagatt actctgcttt ctcaacaaag ataccgtcct gatgccctgc
    49201 ttcattaaag aaccagatgc cgagcgggta gtcttccagc gcaaccaggt acattgtgcc
    49261 ttcactaaac tcctcaactg ccagtaccac tccaggacga cgcggaccgc catccgtttt
    49321 gactgttacc cgatcattca ccttcatcgt tttcctcctg tggctttgtg ccagtgtaga
    49381 acaatttcgt ttttctggca gcgccaagcg cgcgcgagtg ctgattttct cgacggtcta
    49441 tacttaagag atgctaggcg gacttaacga ctggcggcaa caacagagta acggttgcga
    49501 ggaaagatga tgaaaaccgc aaaagtgtac agcgataccg caaaacgtga ggtcagcgtc
    49561 gatgtcgatg ccctgctggc ggcgatcaat gaaattagcg aaagcgaagt tcatcgcagc
    49621 cagaacgatt ctgaacacgt tagcgtcgat ggacgtgaat atcatacatg gcgtgaattg
    49681 gcggatgcct tcgaactgga tattcatgac ttcagcgtct ctgaagtgaa tcgttgaatt
    49741 cacaataaaa aaatcccgac cctgagggtg tcgggatgaa acttgcttaa gcaagaagca
    49801 cttaaaaaat tcgttacacc aggaaatctg atgtgttcat caccttatct gcaatttttt
    49861 tcgctgacaa gaaaatattc gcgctatgaa tgattaacgc tacttatgaa tagaaatgtg
    49921 acgcgcgtca ccccttggtg gctctggtgt caacgggatc gctcccgaaa aaatcatctt
    49981 tgattatttg tgctgttttg agaatcatcc tggtaagggg agtactgcgc gaccatgttt
    50041 tcaattcaac aaccactact ggtttttagc gatcttgatg gcaccctgct ggacagtcat
    50101 agttatgact ggcaaccggc agccccctgg ctcagccgtt tacacgaagc caatattccc
    50161 gtcattctct gtagcagtaa aactttagcg gaaatgctgt acttgcaaaa aatgttgggg
    50221 ctacaaggct taccgctgat tgcagagaat ggcgcagtga tccagcttgc tgaacaatgg
    50281 caggatatag acggttttcc acgcatcatc tcaggtatta gccatggcga aatctgccag
    50341 gttttaaata cgctacgcga gaaagaacat tttaaattca cgacttttga tgatgtcgac
    50401 gatgcaacca ttgccgaatg gacgggatta agccgtagct aggcggcgct gacgcagctg
    50461 catgaggcgt cggtaacgct aatctggcgc gacagtgacg agcgtatggc acaatttatc
    50521 gctcgtctga acgaactggg cttgcagttt atgcagggtg cgcgcttctg gcacgtactg
    50581 gatgcctctg ccggaaaaga tcaggctgcc aattggatta tcgcgaccta tcaacaattg
    50641 tcaggcagac gcccaaccac acttggcctg ggcgatgggc caaacgatgc gcctttactg
    50701 gaggtaatgg attacgcggt gattgtgaaa gggctaaacc gtgaaggggt acatctgcat
    50761 gatgaggatc cggcccgcgt ctggcgaacg cagcgtgaag gaccggaagg ctggcgtgaa
    50821 gggctggacc attttttctc cgcccgttaa gcgttatcgc tcgcgaatac ccgattgcgc
    50881 ccggcctgtt ttgcgaggta aagccgacgg tcggccaatg actggagttg ttcaaaatca
    50941 taatcaccgg tttcctcgct gctacttacc cccagcgagg cactgatgcg tatcgttgta
    51001 ctcttggcga tcagcatctc tttttcattt aacttcaggc gaatacgttc tgcgacctcc
    51061 gcagcctgcg tcagattcgc gcctggcaga atcacacaaa actcctcacc accgacccgc
    51121 ccggcaacgt cctgcgcacg caaggaactg ctaattaatc cggcagcatg agaaagaaca
    51181 cggtcgcccg cctgatgacc aaagcggtca ttaatcgctt taaaatggtc aagatcgacc
    51241 tgaatgacag aaaaaggatg ttggtgcgtc tgacacaatt tagcgagcgg acgggctttt
    51301 tcgaacagtg cgccacggtt atataaacgc gttaaggtgt cgtgccacgc ctgccactgc
    51361 aacgagcttt gcagaacata catgttgctg accatccggc gaatcacata ccaggagagg
    51421 agtaacatgg tggtaaagag cgcccacagc agggttaatg caatgctgat actgccgaaa
    51481 tcgccgcgca cgccttcgct tagcgtatgg acgcgcgcca gcacaccgtc gaaatggtcc
    51541 agacgttccc agctaacata gcgactgtcc atacgaatgc cgccccgcgt gtcatgttcc
    51601 attgcctgcg ccagcaaggc cagttcacga ggatcaaaaa tattccctgt tggatggtca
    51661 ggattggaag aggtcaaaaa tctcagctta ctgtcataga gctgatactc accatcgagg
    51721 tttttatcga tggcgtttct taaaaattgc tgcatagtac gcacgggaat actcatcccc
    51781 agcacgccat accagtagtt attactgtct accggaacac tgacggtaac ctgcggttca
    51841 gtattgctgg cgtgttccgg ttgcgaagtg aaccagcgta ccgcgcggtg acgattttct
    51901 cgttgcgaat ggccgataaa ccagggttgg gtgacatagc cgtaataacg cgttggtaca
    51961 ttgcgcgtaa acaaggtcgg ctgcgtcgaa acgtaaaatc cagcacgcga gacatacatc
    52021 gcctgttcaa ccatcgacga ggagttgtgc gccagtcgca gcaagtaacc aacttccagt
    52081 gcagcggtaa tttcattgtc gaggctttca ttttcgcgag acaggaaatt cccctcgctg
    52141 actaatgcat ccgatacgcc attgactggc agggtgcgcc gtcggttgag ttcgatttgc
    52201 caggcgtgct cgtcgcgatg ctgttcgaac tcggttacgg cattacgcag agaggtgaaa
    52261 tccagtggcg ccacgagagc ttcgcgcatg ccattacgca aaaagatcag tttgtcgacg
    52321 ttatactgca aatgcttatc gagcgcgttg gctacgtttt ccagatgatt acgctagctg
    52381 gagatatagg catcttccag caccaccact tcacgccagg tgagcaaggt ggaaaaaagc
    52441 aataccacga taaagcagag attaacgaca tgaccgggcc ccaggcggcg tgcgagtttt
    52501 ttcaaccagc tctggttttc catttttgtc tcgtgctgca ccctgacgac tcccaacctg
    52561 cttttttatg attctggcat aacccggcaa tagcgtaccg caaaacagcg ataattttat
    52621 aatggtggca tgataagacg cgactatcat tcattgcagg cattgggaca ccaggccgga
    52681 tgtgatccgg cctggtgtta atgataccct ttcaaacttc ttccgcagaa cgactcccct
    52741 cccccggaac aagatcggtt tcatgaaaag cttcccgctt gaccccgtaa ccgtcatacc
    52801 aacgacactc aaccataccg ctggagtatc cagtgacaat catccgcggg ccgccctctt
    52861 taaccgtaac ttcctcacta accataaagc tcatactcgc ctcctttttt cgagtgaaac
    52921 ttgttcacct tagttgaaga tggcgaattt tgcataatag ccagtgcgag tattaatgtg
    52981 cctgaccgcg cattttcgct actgctttca ctcccagcac cacgatgccg ccgatgataa
    53041 atccaagaat cagatttaaa acagtcggta atatcatcgc caccactgca ctttgctgcc
    53101 cggcgatatg ttcaatagcg tgatgcagcg gcgcaatacc atgtaccaca atcccgccgc
    53161 cgacgaggaa catcgccagc gtgccgacaa tcgataatgc tttcatcagc caaggcgcga
    53221 taatcaataa ccctttacct aatgcctgca tcagcgcgct agatttttcc gccagccaat
    53281 accctaggtc atcaatctta acgataaccc ctaccagacc gtagacgccc acggtcacta
    53341 ccagcgcgat gcctgaaagc accagcacct gattaagcaa cggcgcctct gccacaatcc
    53401 ccagcgtgat ggcgacgatt tccgcagaca aaataaaatc ggtacgaatc gcccctttaa
    53461 ttttgtcctt ttcaaacttc agcggatcct gcgccgccag cttctccaga cgctgctggc
    53521 tctgcgctgg atcttcttta tgtttacgcg cctccagcat atgcagcact ttctctaccc
    53581 cttcaaagca gagaaacgcg ccaccaatca tcaacagtgg cgtaatcgcc cacgggataa
    53641 acgcactgat gatcagcgcc agcggcacca gaatcacttt attgatcagc gatcctttcg
    53701 ccacgcccca gaccacggga agttcccggt tggcccgcac gcctgaaact tgttgcgcat
    53761 tgagcgataa atcatcccct aatacaccgg cggttttctt cgccgccagt ttgcccatca
    53821 cagagatatc gtccagcagt gtggcgatat catcgagcaa cgtcagtaaa ctactcccgg
    53881 ccaaaagcat tccctctcat ttttattgtt gaagggtgta agtatgaagc aaaaacggca
    53941 gctctgatac ttgcttcaca ggtcaacatt tatttaacaa tcaagtgcaa ttatatctct
    54001 cgccagcaga atgattttca cgtttactat gaaccacctt tttatttccg ccgtgaggga
    54061 ttatgcgttt ccgccagttg ttaccgcttt ttggcgcgct gtttgcgttg tatatcattt
    54121 ggggctcaac ctattttgtc attcggattg gcgtgggaag ctggcctccg ttaatgatgg
    54181 cgggcgttcg attccaggca gccggtattt tattgctggc atttttgcta ctgcgcggac
    54241 acaaacttcc cccgctacgt ccgctgctca atgccgcgct gattggcctg ttattgctgg
    54301 ctgtcggtaa tggcatggtg acggttgccg aacatcaaaa tgttccttcc ggcatcgccg
    54361 ccgtagtgat tgcaaccgtg cccctcttta ccctgtgctt cagtcgcctg tttggcatta
    54421 aaacgcgcaa actggaatgg gtgggtattg ccattgggct tgccggaatc atcatgctca
    54481 atagcggtgg aaatttaagc ggcaatccgt ggggcgcgat tctgatttta atcggctcga
    54541 ttagctgggc gtttggctca gtttatggct cgcgcattac cttacctgta gggatgatgg
    54601 cgggtgcgat tgagatgctg gcggcaggcg tggtgttaat gatcgcatcg atgattgcgg
    54661 gtgaaaaact gacggcgctc ccttccctgt caggcttcct tgcggtcggc tatctggcgc
    54721 tgtttggttc gattatcgcc attaacgctt atatgtattt aatccgtaat gtcagtccgg
    54781 ctctcgccac cagctacgcc tacgttaacc cggtggtcgc ggtcttgctg ggtacgggac
    54841 tgggtggcga aacgctgtcg aagattgaat ggctggcgct cggcgtaatt gttttcgcgg
    54901 tggtactggt cacgttggga aaatatctct tcccggccaa acccgttgtc gcgccagtta
    54961 ttcaggatgc atcaagcgag taaatgaatc ccctgcgtgt cgatctgcgc gctggcacct
    55021 tcgccgcaga tccactcttc cagacgctcg gtaagcgccg catccgtcag cttctcgcgc
    55081 ctacgtaacg cacactccca gacaatcagt acgcgccagc cgagctcctg caagcgactg
    55141 atatcgcggc gatcgcgttc aacattttta cctatcttct ccagccagaa ctcagttcga
    55201 gtcgcaggca ctttaaacag atagcagtga tgatgatgcc agaagcagcc gtgggtaaat
    55261 atcacgcagc gatattcatc aacgacaaaa tccgggcgtc cgggcagact ggcgtcctga
    55321 acgcgaaacg caaggccctg cccggttaac aaactggcga ggcgcttctc aatcgccgta
    55381 tcacgcgtgg caatcgcgcg catatttttg ctgcgggtgg ccttatcgtg aacgtcgacc
    55441 atgttgtgcc tcttgctgac gcaacgccac cgcctgtttg atttttggct caagcagttt
    55501 tgccaccgcg gcaaagaccg gcacgaccac cgagttgccg aactggcgat aggcctgagt
    55561 gtccgaaacc ggaatgcgga atttcgcttc tcccggcgct tcaaaaccca ttaagcgcgc
    55621 gcattcacga ggcgttaacc gacgtggacg atgttgctga ttcagcggat cgtcaaagtc
    55681 tttctcaccc gtggccatat cccaaccgcg atcgattaaa atttccgcgc catctttgta
    55741 ataacgagca gacagcgtac gggtgacgct ttgcggattg ttcggataaa ccattccata
    55801 accgaagccg ttaccgcgcg cctgatgttt tttcgcatat cgatagaggt acttccacag
    55861 caccggcgtc aggatatatt tcgcctcaac catcgggtcc agcagctgcg ccagcgtcac
    55921 tcgctgtgca gggaaacatt cgctgatatc acgcagagta aaatcggctt tcagattcag
    55981 atcgcgacga aaacccacca gcacgatgcg ttcacggtgc tgcggcagga aatgtttacc
    56041 atcgatgatt ttcggatcgt ccggcccgtt atcttctgca tcggcaacgt catagcccag
    56101 ttcgtccagc gtctgcatga tgatgcggaa cgtttttccc ttatcgtgac tcttcaggtt
    56161 tttgacgttt tcgagcacaa acatcgccgg acgacgcgcg tcgataatgc gcaccacatc
    56221 aaagaacagc gtgccctggg tatcgcaggc aaaaccgtgc gcccgcccga gcgagttctt
    56281 tttcgatacg ccagccagcg aaaatggctg acaagggaaa ccggccagta aaacatcgtg
    56341 ttcaggaatg tgttgacgaa tatgttccgc cgccgcctca tcactcacgc cttcttgatg
    56401 actgagggtg atgtcgcgga tatcttcatt aaaatgatgc gtcgccgggt cgcaataatg
    56461 gttggcttta taagtgcgta ccgcatgttt gttccattcg ctggtaaaca cgcactgtcc
    56521 accaatcgat tcaaaaccac gacggatgcc gccaattccg gcaaatagat cgataaagcg
    56581 aaacgcataa tgcggatgat gttccggtgg tttgggtaac aacgtttgca gatgggcaaa
    56641 ctctttctca cttaaacggt gccatgccga gtcattcgcc agcgcacgtt ttaaaattgc
    56701 cgcgctccag tgattctcac ccacaccatt aagctgcgcc accagcattt taacatcata
    56761 aatttgcagc agtttctcca gcattgcctg tgcggcattc ccggtgctgt atgaatcggt
    56821 tactgatata ttttcctgca tagatttaac cggccatctg agatggccga caggttaaca
    56881 taattactgc cgtttagggc cacgcgctca gccagcgggt gaaaacgcat ctatcacctt
    56941 gtgatcgata ccttcattct cccccgccag ttcggcaccg agcttcgcca tcaaacagcg
    57001 ccaagctcaa tgcccctgaa acggcaaata cgcgcgccag gccaatcgcc cccagggctt
    57061 ccagccgatc cgcatcctgg acgattttag cttccgttgt taagggggcg atttgcgcgc
    57121 tgaaactgtg agcggcaatg gcatgacaaa cggcctcaat tttctccgcc ggaaattgca
    57181 cgaactcttc acggagcagg cgacgcgtct cttctgccgc caggattgaa gaacgctgcc
    57241 gttgcggatg atttttcgcc aggctcacga tatcgtgaaa ataacaggcg gttaaaatca
    57301 ccagcatatc gacatcgtca tctgccgcca gtttctgtgc cgtagcccag acacggcgaa
    57361 aatgacacag gtcatgcgcc gcgtcctgat gttgatgatg gtttttcaac cagttctcga
    57421 actgtgcctg ccagtgttgt aactccatac gcgctccgtt gccaaagagt cgcaacctta
    57481 gcagttttta ttcatagcgt ctgcaacgtg cgggacgagg tcgggtgtac catgcaatgc
    57541 cgcctaacaa ggcaccaacc agggccataa acagaaatcc gaccaatgct cctaaccact
    57601 tccccgcggt agatcctaaa tcactttcaa atcccgacac gggggcatcg gggatccggc
    57661 tcatcaccca gatgtatcgc gccattgccc agacgaaata gccacaccat gtatagaaag
    57721 cccacaatga atcgccacgg ataatctaga cacttccgag ccgttgataa tactggtttt
    57781 catattctgt cggtgacatc tgttcgctag aaccatgccg acgcttactg ttataaaaca
    57841 tttcgatgta atcaaaaata tcactgcggg cttcttcccg cgttccgtag atctttttct
    57901 ttatccgttc acgtttcaac aactggaaaa aactttctgc aaccgcatta tcatggcagt
    57961 taccgcgacg gctcatgcta ccctccaggc cgtgtgattt caggaacgac tgccactcat
    58021 ggcttgtgta ctgactgccc tgatccgaat gaaccagcac ctgtttttcg
//
LOCUS       DAEEUE010000015        55466 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.015, whole genome shotgun sequence.
ACCESSION   DAEEUE010000015 DAEEUE010000000
VERSION     DAEEUE010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55466
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.015"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="KLK84_000972"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="KLK84_000972"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBE2008399.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="KLK84_000973"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="KLK84_000973"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBE2008400.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="KLK84_000974"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="KLK84_000974"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBE2008401.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="KLK84_000975"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="KLK84_000975"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HBE2008402.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="KLK84_000976"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="KLK84_000976"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HBE2008403.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="KLK84_000977"
     CDS             7826..9559
                     /locus_tag="KLK84_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HBE2008404.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="KLK84_000978"
     CDS             9708..10559
                     /locus_tag="KLK84_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE2008405.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="KLK84_000979"
     CDS             complement(10546..10887)
                     /locus_tag="KLK84_000979"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBE2008406.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="KLK84_000980"
     CDS             complement(10889..11767)
                     /locus_tag="KLK84_000980"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HBE2008407.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11769..14030)
                     /locus_tag="KLK84_000981"
     CDS             complement(11769..14030)
                     /locus_tag="KLK84_000981"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBE2008408.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDTYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEI"
     gene            complement(14081..14401)
                     /locus_tag="KLK84_000982"
     CDS             complement(14081..14401)
                     /locus_tag="KLK84_000982"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBE2008409.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="KLK84_000983"
     CDS             complement(14416..15495)
                     /locus_tag="KLK84_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBE2008410.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..16907
                     /locus_tag="KLK84_000984"
     CDS             15804..16907
                     /locus_tag="KLK84_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBE2008411.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            16908..18305
                     /gene="ptsP"
                     /locus_tag="KLK84_000985"
     CDS             16908..18305
                     /gene="ptsP"
                     /locus_tag="KLK84_000985"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBE2008412.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="KLK84_000986"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="KLK84_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20091
                     /gene="gldA"
                     /locus_tag="KLK84_000987"
                     /pseudo
     CDS             18990..20091
                     /gene="gldA"
                     /locus_tag="KLK84_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20366..20983
                     /locus_tag="KLK84_000988"
     CDS             20366..20983
                     /locus_tag="KLK84_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HBE2008413.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVASDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(21010..21915)
                     /gene="yijE"
                     /locus_tag="KLK84_000989"
     CDS             complement(21010..21915)
                     /gene="yijE"
                     /locus_tag="KLK84_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HBE2008414.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(22009..24189)
                     /gene="katG"
                     /locus_tag="KLK84_000990"
     CDS             complement(22009..24189)
                     /gene="katG"
                     /locus_tag="KLK84_000990"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HBE2008415.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24518..25408)
                     /gene="metF"
                     /locus_tag="KLK84_000991"
     CDS             complement(24518..25408)
                     /gene="metF"
                     /locus_tag="KLK84_000991"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBE2008416.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25757..28189)
                     /gene="metL"
                     /locus_tag="KLK84_000992"
     CDS             complement(25757..28189)
                     /gene="metL"
                     /locus_tag="KLK84_000992"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HBE2008417.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28192..29352)
                     /gene="metB"
                     /locus_tag="KLK84_000993"
     CDS             complement(28192..29352)
                     /gene="metB"
                     /locus_tag="KLK84_000993"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBE2008418.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29629..29946
                     /gene="metJ"
                     /locus_tag="KLK84_000994"
     CDS             29629..29946
                     /gene="metJ"
                     /locus_tag="KLK84_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HBE2008419.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30130..30738
                     /locus_tag="KLK84_000995"
     CDS             30130..30738
                     /locus_tag="KLK84_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HBE2008420.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30799..31011)
                     /gene="rpmE"
                     /locus_tag="KLK84_000996"
     CDS             complement(30799..31011)
                     /gene="rpmE"
                     /locus_tag="KLK84_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBE2008421.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31214..33412
                     /gene="priA"
                     /locus_tag="KLK84_000997"
     CDS             31214..33412
                     /gene="priA"
                     /locus_tag="KLK84_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBE2008422.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRMRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33568..34593
                     /gene="cytR"
                     /locus_tag="KLK84_000998"
     CDS             33568..34593
                     /gene="cytR"
                     /locus_tag="KLK84_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HBE2008423.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQEHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34685..35644
                     /gene="ftsN"
                     /locus_tag="KLK84_000999"
     CDS             34685..35644
                     /gene="ftsN"
                     /locus_tag="KLK84_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HBE2008424.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35737..36267
                     /gene="hslV"
                     /locus_tag="KLK84_001000"
     CDS             35737..36267
                     /gene="hslV"
                     /locus_tag="KLK84_001000"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HBE2008425.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36277..37608
                     /gene="hslU"
                     /locus_tag="KLK84_001001"
     CDS             36277..37608
                     /gene="hslU"
                     /locus_tag="KLK84_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HBE2008426.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37675..38601
                     /gene="menA"
                     /locus_tag="KLK84_001002"
     CDS             37675..38601
                     /gene="menA"
                     /locus_tag="KLK84_001002"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBE2008427.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38694..39179
                     /gene="rraA"
                     /locus_tag="KLK84_001003"
     CDS             38694..39179
                     /gene="rraA"
                     /locus_tag="KLK84_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HBE2008428.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39264..39509)
                     /gene="zapB"
                     /locus_tag="KLK84_001004"
     CDS             complement(39264..39509)
                     /gene="zapB"
                     /locus_tag="KLK84_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HBE2008429.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39935..40780
                     /gene="glpF"
                     /locus_tag="KLK84_001005"
                     /pseudo
     CDS             39935..40780
                     /gene="glpF"
                     /locus_tag="KLK84_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40820..42310
                     /gene="glpK"
                     /locus_tag="KLK84_001006"
     CDS             40820..42310
                     /gene="glpK"
                     /locus_tag="KLK84_001006"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBE2008430.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDVLGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42540..43550
                     /gene="glpX"
                     /locus_tag="KLK84_001007"
     CDS             42540..43550
                     /gene="glpX"
                     /locus_tag="KLK84_001007"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HBE2008431.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43647..44393
                     /gene="fpr"
                     /locus_tag="KLK84_001008"
     CDS             43647..44393
                     /gene="fpr"
                     /locus_tag="KLK84_001008"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HBE2008432.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44398..44826)
                     /gene="uspD"
                     /locus_tag="KLK84_001009"
     CDS             complement(44398..44826)
                     /gene="uspD"
                     /locus_tag="KLK84_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HBE2008433.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44853..45152)
                     /locus_tag="KLK84_001010"
     CDS             complement(44853..45152)
                     /locus_tag="KLK84_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HBE2008434.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45364..45804)
                     /locus_tag="KLK84_001011"
                     /pseudo
     CDS             complement(45364..45804)
                     /locus_tag="KLK84_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45905..46504
                     /locus_tag="KLK84_001012"
     CDS             45905..46504
                     /locus_tag="KLK84_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HBE2008435.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46612..47379
                     /gene="tpiA"
                     /locus_tag="KLK84_001013"
     CDS             46612..47379
                     /gene="tpiA"
                     /locus_tag="KLK84_001013"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBE2008436.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47434..48189)
                     /gene="cdh"
                     /locus_tag="KLK84_001014"
     CDS             complement(47434..48189)
                     /gene="cdh"
                     /locus_tag="KLK84_001014"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HBE2008437.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48296..49285)
                     /gene="sbp"
                     /locus_tag="KLK84_001015"
     CDS             complement(48296..49285)
                     /gene="sbp"
                     /locus_tag="KLK84_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HBE2008438.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     SHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49605..50567)
                     /gene="pfkA"
                     /locus_tag="KLK84_001016"
     CDS             complement(49605..50567)
                     /gene="pfkA"
                     /locus_tag="KLK84_001016"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBE2008439.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50748..51650)
                     /gene="fieF"
                     /locus_tag="KLK84_001017"
     CDS             complement(50748..51650)
                     /gene="fieF"
                     /locus_tag="KLK84_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HBE2008440.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51798..52298)
                     /gene="cpxP"
                     /locus_tag="KLK84_001018"
     CDS             complement(51798..52298)
                     /gene="cpxP"
                     /locus_tag="KLK84_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HBE2008441.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52448..53146
                     /gene="cpxR"
                     /locus_tag="KLK84_001019"
     CDS             52448..53146
                     /gene="cpxR"
                     /locus_tag="KLK84_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HBE2008442.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53143..54516
                     /gene="cpxA"
                     /locus_tag="KLK84_001020"
     CDS             53143..54516
                     /gene="cpxA"
                     /locus_tag="KLK84_001020"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HBE2008443.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54622..55296)
                     /gene="yiiM"
                     /locus_tag="KLK84_001021"
     CDS             complement(54622..55296)
                     /gene="yiiM"
                     /locus_tag="KLK84_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HBE2008444.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13516 a        15032 c        14165 g        12753 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcagttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttac attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct agatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggt gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcagcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggatgca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggcgtcag cgccggatta agctgtgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtaccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgaaggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctgacagc cagctttaaa acgccgcgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt taggttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc
    19741 ggcggctcac gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca
    19801 cggtgaaaag tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag
    19861 gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa
    19921 ctggatatta aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca
    19981 gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca
    20041 ctgctggtag ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca
    20101 aactcccggc ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg
    20161 cctacgcaat ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc
    20221 actccgcatc cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt
    20281 tatccgcttc tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg
    20341 actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac
    20401 ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca
    20461 ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt
    20521 ttacgatccg gcgtatatgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg
    20581 tgtatgttcc gatgtggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact
    20641 ggttcacgaa gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg
    20701 cccggacagc aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca
    20761 caacaaaaca cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc
    20821 ctggcgatta gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga
    20881 cggtacgccg ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt
    20941 tagctggcgg atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga
    21001 agaaaaaatt cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc
    21061 cagtgccagc acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag
    21121 cagccaccag gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat
    21181 actggcaggc aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag
    21241 aatcgcactg taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg
    21301 caccagtaaa gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa
    21361 cgataataaa tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc
    21421 cccccaacta acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag
    21481 ttgccacggc tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg
    21541 cccacgtcgc aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat
    21601 ggtatagctc aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccaa
    21661 accaaccatc ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg
    21721 gcgcattccg cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca
    21781 gcgtaaggcg gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca
    21841 gctataactc cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt
    21901 tcctgcggca gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta
    21961 aagaaacttc agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc
    22021 gaaacggtcg aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc
    22081 gtgggcatcg ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa
    22141 taccagatcc gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc
    22201 gaacagctct ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac
    22261 gaagaagtca ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc
    22321 gaagttggca cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag
    22381 cgtcagttgc tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc
    22441 gcgatagtta cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt
    22501 ctgatcctga cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc
    22561 ggcgcttgcg gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga
    22621 ggctttacca gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc
    22681 gttcacatcc cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg
    22741 tttgtcgcca ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac
    22801 agacagacca gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt
    22861 gtagatcggc tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc
    22921 gatgtagcga gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa
    22981 ggcttcgttg aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg
    23041 atcaaaacgc agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc
    23101 gaacggatcc gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg
    23161 actgcgggtc tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg
    23221 ggtcggcgtc tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc
    23281 gctgccgtaa gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc
    23341 aggacctacg tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc
    23401 aatcagcgcc acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc
    23461 tgctgccgca gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag
    23521 gcccatctcg gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag
    23581 ccaggctttt tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc
    23641 ggcaccaaaa ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat
    23701 aaacaggtcg gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg
    23761 acgcgcttta tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg
    23821 accacgaccc gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc
    23881 catacgaata aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc
    23941 tgtcagcagg gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt
    24001 gcggtagtca aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc
    24061 aacacggagt tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc
    24121 gtgaccgccc tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga
    24181 cgtgctcatc aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag
    24241 aagttataat tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa
    24301 agagatgtag atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa
    24361 atgctttata aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg
    24421 ctgacaacct tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt
    24481 tgtgatcact gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca
    24541 gcgtatggca aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt
    24601 tcactccttc acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt
    24661 tgcgggtttc ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac
    24721 gcacgttggt catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc
    24781 ccgggatgat ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc
    24841 tttcgacatc gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt
    24901 tcagattaag caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg
    24961 ccacggatat atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca
    25021 tttctggctt accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac
    25081 cgttattcca gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag
    25141 taagatgcgg tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg
    25201 tacggtcgcg ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc
    25261 tgctaaggcg atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga
    25321 aaaactcgaa cgaaacatta atctgcccct ggacttctgc caggctctga ttcagggcat
    25381 cccgctggct ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg
    25441 ttgtgttgtg cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa
    25501 gggcgtcaac cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact
    25561 tcaggatggt aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac
    25621 taaccatgta cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc
    25681 tatgataatt caatatattt cattagaaac catagacccg aaaacatgag taccgggcat
    25741 tattaaattt ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc
    25801 cggcggtgac gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc
    25861 agcggctttc gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt
    25921 cacgcaccgc ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc
    25981 gcagcaccag ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt
    26041 catcgccatt ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg
    26101 attccacacg tacctgatcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca
    26161 gcttgcgcat tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct
    26221 gctgccacgc ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca
    26281 gccaggagag cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga
    26341 tcagatcgcg cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc
    26401 agtgacgccc ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg
    26461 ctcccgccag tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact
    26521 gatcagcaag ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg
    26581 cgcgcatcca caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg
    26641 ctaacgcgcg gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca
    26701 cgcctgccag cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga
    26761 acagttccag ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt
    26821 tttctgcgcg gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc
    26881 gcagtactgc caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc
    26941 ctttcagttg ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga
    27001 ccatcgccac cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt
    27061 cgaggatttt cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc
    27121 gatcgttatg cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga
    27181 tctctttatg cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga
    27241 catcatcgtg gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc
    27301 gcgtggaacc ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag
    27361 aaactggctg taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg
    27421 cctcatccag acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac
    27481 tgtatacccc ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga
    27541 tttgtgtcgc ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt
    27601 tacggctgat aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt
    27661 gcagcaacgg gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta
    27721 aaaactcacg ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca
    27781 tcaggcgcgc cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa
    27841 taccactgtc gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc
    27901 tatcggcttc ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg
    27961 tttgttgaac ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca
    28021 actggttagt ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact
    28081 ccgccataat gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa
    28141 atttatgcag ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt
    28201 gtttgcagcc cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc
    28261 ggtggagata cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat
    28321 gcctgcatgt gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc
    28381 cgccagcgta aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc
    28441 cagttcaaaa ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc
    28501 ctgattttcc ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata
    28561 tttcacaatc gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa
    28621 cccgcgtagc agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca
    28681 ccaggcaagt tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc
    28741 agagtgaccg ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag
    28801 cggattttgt aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc
    28861 ccttgccaga tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct
    28921 ttctaccagt accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc
    28981 ttgatcaaca aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata
    29041 gctaccgccg tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt
    29101 cgttaccagg tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc
    29161 cagttctgcc agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc
    29221 atgcgcgcgc ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg
    29281 gacaacgcaa ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg
    29341 tttacgcgtc atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt
    29401 aaacattgtg ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt
    29461 gagcaaatcc caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca
    29521 cgatgtcacg gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa
    29581 attgtggggt ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg
    29641 agcggcgaat atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag
    29701 attacggttt ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt
    29761 caggtgaaca acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat
    29821 gcctttaccg ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa
    29881 atcccggaag cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa
    29941 tactaaagcg gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc
    30001 ctacgtcata ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac
    30061 gccgcatccg gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga
    30121 cgtttaacta tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt
    30181 gcctggcagc cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa
    30241 gcgatccaac tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac
    30301 aaaaagccct acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg
    30361 attgcccatg gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt
    30421 gaacaacagc aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc
    30481 agcttctcat ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag
    30541 aacgcgctgg gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac
    30601 ccgctggtcc aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg
    30661 gtagtttcac cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg
    30721 ccgctgtttt cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct
    30781 tttttcggaa atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc
    30841 aacacggcca ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct
    30901 gcacacgtcg aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca
    30961 agagcagcta gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc
    31021 tcggtttaag gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag
    31081 tatagcttca atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg
    31141 tatgcaaact gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag
    31201 tcaggatgat gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt
    31261 tgactatctg ctgccagaag gcatgacggt taaagctggg tgtcgcatgc gcgtgccgtt
    31321 tggcaaacag caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc
    31381 gctcactgag ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc
    31441 cgtctggcga ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct
    31501 gtttcatgcc ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg
    31561 gtactggttt gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc
    31621 caagcaacaa caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc
    31681 cacgctcgaa tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt
    31741 agcaagtgaa acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg
    31801 gttgcgattg aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac
    31861 tttttctgcc tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag
    31921 tgtactggaa aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg
    31981 cctgacaccg caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct
    32041 gcattccggt ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga
    32101 ggcggcgatt gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt
    32161 gattgtcatt gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca
    32221 tgcccgcgac ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc
    32281 cgcaacgccc gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg
    32341 cctgacgcgc cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg
    32401 tcagaaggtg caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca
    32461 ggccaacaat caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg
    32521 ccacgactgc ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca
    32581 ggcgcaacag catttgcgct gtcaccactg tgacagtcag cgtccagtgc cgcgccagtg
    32641 cccttcctgc ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca
    32701 gacgcttgcg ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag
    32761 ccgcaaaggg gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt
    32821 gatcggcaca caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt
    32881 actggatgtg gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca
    32941 gctttacacc caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct
    33001 gcaaacgcac catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgacgc
    33061 ctttgccgaa caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca
    33121 tgtgattgtg cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact
    33181 gcgtaatctg atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt
    33241 tccggcactg gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc
    33301 ttcccgcgtg cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc
    33361 ggattcccgc aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta
    33421 cgatgcgagg cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac
    33481 cgttttcacg cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac
    33541 gcctgacgtg ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc
    33601 atgaaagacg ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg
    33661 aatcccgata aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg
    33721 ggttatttac cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg
    33781 gtgattgtcc cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt
    33841 acggcggcaa atcacggata tctggtgctg attggcgact gtgcacatca aaatcagcag
    33901 gaaaaaacct ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt
    33961 tcaaggctgc cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg
    34021 gcgaacgaat ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc
    34081 gcatttgatg cagtaaatta tttatatgag caagagcata aacggattgg ctgtatagcc
    34141 ggtcccgaag agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt
    34201 cgctgcggaa ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc
    34261 ggaagcaaag caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc
    34321 catagcgatg tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc
    34381 ccggaagacc tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg
    34441 ccgctgacaa ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg
    34501 cttgatcaaa tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt
    34561 atcatccggg gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg
    34621 gtcaaaggcc cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac
    34681 gatagtggca caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa
    34741 gagcacctca cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat
    34801 tgctgccgcc gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa
    34861 agaagagtcc gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc
    34921 agaagaacgc tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc
    34981 cacagaacct tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg
    35041 tcagcttctt gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt
    35101 tccgtggaat gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc
    35161 gcagcaatta gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg
    35221 gcagcagcag acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa
    35281 accagcttct actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc
    35341 gcagtcgaag ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac
    35401 ggcggagaaa aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga
    35461 acaggcagag acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac
    35521 caacaatggc tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag
    35581 caccctcaat cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg
    35641 ttgaaaccct caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg
    35701 gtttgtactc tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc
    35761 cgtaacggcc atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg
    35821 aaaggcaacg tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg
    35881 ggcggtactg cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat
    35941 cagggccatc tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg
    36001 ctgcgcaaac ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc
    36061 ggtaacggtg acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct
    36121 tacgcccagg ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt
    36181 gctgaaaagg cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc
    36241 atcgaagaat taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa
    36301 atcgtcagcg aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg
    36361 attgctctgc gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg
    36421 accccgaaaa atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt
    36481 cgtctggcta agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa
    36541 gtgggctacg tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg
    36601 aaaatggtac gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa
    36661 gaacgtattc tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag
    36721 cagcaggaac cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt
    36781 gatgacaaag aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct
    36841 cctccgggca tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc
    36901 cagaagcaaa aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa
    36961 gaagcggcga aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag
    37021 cagcacggga tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc
    37081 ggtccggatg tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc
    37141 accgtttcca ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc
    37201 gcgttccaga ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc
    37261 cgcgttgaac tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat
    37321 gcctctatca ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt
    37381 accgactccg gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa
    37441 aacatcggtg ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac
    37501 gacgccagcg atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat
    37561 ctggatgcgt tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc
    37621 attttcatca ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact
    37681 gaacaacaaa ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc
    37741 ctcgcctttg ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat
    37801 ccgctggtcg ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc
    37861 gccaatgatt acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta
    37921 cgcggcatgc aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc
    37981 gtcgtgctca tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc
    38041 gattttgtcg gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc
    38101 gtgggcaatc gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt
    38161 ggctggttga gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg
    38221 atccttccgg cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg
    38281 cgtgatatca atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa
    38341 gtgaacgcgc gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg
    38401 tttaatctct tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta
    38461 ctggtgaagc aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg
    38521 ctggaacgta ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc
    38581 ctaagccagt gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca
    38641 gcccacatag cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat
    38701 acgatacttc cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt
    38761 tctccaactt tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg
    38821 aggacaacgg gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg
    38881 tcgatggcgg tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag
    38941 tacaaaatga atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg
    39001 aagagttgga tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag
    39061 gcattggcga aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc
    39121 atctttatgc cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat
    39181 aagaaaggca ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa
    39241 attacgcgaa agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga
    39301 cgttcctgcc agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg
    39361 cgctgatgct gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct
    39421 tcgatttcca tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt
    39481 ttctcaaaca cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat
    39541 aaaaaatcct cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag
    39601 ctccgattgt atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt
    39661 gccatacttt tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa
    39721 tttgttgcgc gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg
    39781 cgataacgct cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg
    39841 ctcgttaacg ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg
    39901 catacaacaa acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg
    39961 ccagtgcatt gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt
    40021 tgcagcacta aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg
    40081 actgggggtg gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc
    40141 cgctgttacc attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt
    40201 tatcgtttca caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta
    40261 caatttattt ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt
    40321 tgatctggct ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt
    40381 cgcagttgag atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga
    40441 tggcaacggt gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt
    40501 cattggcgca tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg
    40561 tccgaaagtc tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga
    40621 cattccttac ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt
    40681 tgcctaccgc aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa
    40741 ggaaaccaca actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa
    40801 acatgactga aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt
    40861 cgtaatggat cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta
    40921 cccaaaacca ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac
    40981 gctggtagaa gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat
    41041 tacgaaccag cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa
    41101 cgccattgtc tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg
    41161 tttagaagat tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac
    41221 caaagtgaag tggatcctcg accatgtgga aggctctcgc gagcgtgcac gtcgtggtga
    41281 attgctgttt ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca
    41341 tgtgaccgat tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg
    41401 ggacgacaaa atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg
    41461 ttcttccgaa gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat
    41521 ctccgggatc gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg
    41581 gatggcgaag aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc
    41641 ggtgaaatca gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa
    41701 ctatgcgttg gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga
    41761 gatgaagttg attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac
    41821 caatggtgtg tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta
    41881 tgcgcgcggg gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc
    41941 gacgctggag tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc
    42001 tggtatccgt ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat
    42061 gcagttccag tccgatgttc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac
    42121 cgcattgggt gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga
    42181 gctgcaagag aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg
    42241 taattaccgt tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca
    42301 cgacgagtaa tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct
    42361 tacgcgtctt atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca
    42421 cgccgcatcc ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa
    42481 taatcccacc cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta
    42541 tgagacgaga acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct
    42601 acaaatggtt aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc
    42661 gtattatgct caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg
    42721 acgaagcacc gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag
    42781 atattgctgt tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc
    42841 tggcggtgct ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg
    42901 agaagctgat tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg
    42961 ataacctgcg caatgtagca gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga
    43021 ttctggctaa accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg
    43081 tatttgctat tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg
    43141 aagttgacgt gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga
    43201 tccgcgcatt agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg
    43261 acaacgaaga gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca
    43321 tcgaagccgg taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct
    43381 ctgccaccgg tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg
    43441 cgactaccga aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca
    43501 tccactatct ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat
    43561 cgattgagcc ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg
    43621 caagtcaatc aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag
    43681 tgcagaactg gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta
    43741 ccgccgggca atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct
    43801 actcctatgt taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg
    43861 atggcaaatt aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta
    43921 gcgaagcagc tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc
    43981 tggcaaccgg tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag
    44041 atcgcttcaa aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt
    44101 tgccactgat gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg
    44161 tcagtcggga aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg
    44221 gggaactgga aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt
    44281 gcggcaatcc acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga
    44341 cgaaacattt acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta
    44401 cttatcgata aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata
    44461 tgccggcatc aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg
    44521 ctctttttgc ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt
    44581 tgtcttcggc cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt
    44641 caacaattga agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa
    44701 gccatcatca atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc
    44761 tttattcacc agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata
    44821 agccatgttt aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca
    44881 aaaataatca tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc
    44941 tccagatcct taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg
    45001 gcaagctctt tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc
    45061 gaaaacttac tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct
    45121 ttagtactgc atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg
    45181 tcgattacga cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat
    45241 ggattaccat tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat
    45301 tgtcatttgc atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa
    45361 aggttaatta cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata
    45421 tttattttcg ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat
    45481 caacgtcggg acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt
    45541 acccgccagc agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg
    45601 atcatgcaag cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa
    45661 cgccgcggtc tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc
    45721 tgcgaaccac aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga
    45781 gaataaccat tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta
    45841 cccttttgac aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact
    45901 gtcaatgaag ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac
    45961 aacggcgcat gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc
    46021 ccctactttc gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag
    46081 cctgccactg aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg
    46141 tgctgcaagt aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt
    46201 aaaaatcaaa agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc
    46261 gaaagcgaaa gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac
    46321 caaaacacaa agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta
    46381 ttacaccgag acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct
    46441 gaccttcgct gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa
    46501 atgacaaaaa gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc
    46561 cctgcggggc ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat
    46621 cctttagtga tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt
    46681 tctaacctgc gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg
    46741 gaaatgtaca tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg
    46801 caaaacgtgg acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg
    46861 aaagacatcg gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa
    46921 gagtctgacg aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg
    46981 gttctgtgca tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc
    47041 gcacgtcaga tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt
    47101 atcgcttacg aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca
    47161 caggctgttc acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa
    47221 caagtgatca ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct
    47281 cagccagaca tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca
    47341 gtgatcgtta aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc
    47401 atatccggca cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc
    47461 ctggatttcc tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa
    47521 cacaaacgaa ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg
    47581 cgcctcaggc acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc
    47641 cgttacccga cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg
    47701 gctgctgatg ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga
    47761 gatatgaata tgaaaatggt tttgcgtacg cccggtgcgg gagttgatcg ccaaagaaac
    47821 cgcgcgatcg ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc
    47881 caaccaaaag aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat
    47941 acgatacgtt ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta
    48001 tccggcattg ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg
    48061 caaacattcc tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca
    48121 gtaaccaata ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc
    48181 ttttttcatc tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac
    48241 aaataaaaaa cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc
    48301 gtttgctgat ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc
    48361 agccgccgaa ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact
    48421 ttttcgctac ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt
    48481 ctggcgagta gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga
    48541 ccactttatc gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga
    48601 tttcgaattt atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga
    48661 tcagtacatc gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat
    48721 ccagaacttc gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt
    48781 tgttgttgtg atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag
    48841 agcttttcgg attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat
    48901 ggatctgctt cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg
    48961 agttatccgg cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt
    49021 ccacgtcata ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg
    49081 tcgcttgttt acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct
    49141 gtttccagtg ggagctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat
    49201 atgaaacgtt aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg
    49261 ttaaccctac gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa
    49321 agcgtgccag aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg
    49381 gtatatgttg agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg
    49441 ctttgtccat gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc
    49501 aataaattgc agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct
    49561 gtcatcggtt tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc
    49621 gcgcagtcca gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg
    49681 tcgtggtgaa ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc
    49741 agcagatcga tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga
    49801 gaaccaccgc gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc
    49861 tcgatgaaat gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg
    49921 tgttttttac ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat
    49981 tcaacttccg gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga
    50041 tcgccacaat aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg
    50101 tcacgcagac ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg
    50161 tagtcagtgc ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc
    50221 atttcggtca gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg
    50281 tcgataccac gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat
    50341 tccgggaaac gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg
    50401 taacggtcta gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc
    50461 attacttcca gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg
    50521 cctggcgcat caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa
    50581 ctttggaatg caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa
    50641 cgaatgtgca aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg
    50701 gcggtatttt atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata
    50761 gaccgtttac cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat
    50821 cccggaaaac gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc
    50881 aaaggcagag agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc
    50941 cgcgtgcgaa gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt
    51001 tcttgccgtt cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc
    51061 atgcgtaacg cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg
    51121 cgatgccagc cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca
    51181 tcagactggt aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc
    51241 caccgctgaa acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc
    51301 ccgacgcctg gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac
    51361 aggaatagtg ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct
    51421 ttaccgtgac caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt
    51481 aaattcgtca acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc
    51541 accgacccgg tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc
    51601 gtcgcagcaa tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc
    51661 gctatcaact gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc
    51721 aaattgagga taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc
    51781 aaggaaaaca gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat
    51841 gaactttttt gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg
    51901 tttaaaaccg cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc
    51961 atctcaacct gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg
    52021 ttttcatcaa aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta
    52081 acaggaggct gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt
    52141 tcggttaaac ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca
    52201 cccggatgcc agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc
    52261 agcgttgagg ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct
    52321 gtcgcgattc aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta
    52381 aaacaacgta aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat
    52441 ttaaacaatg aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa
    52501 ggagctgctc gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct
    52561 tgatcttctg gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa
    52621 tggtatcgac acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac
    52681 ggcgcgcggc agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct
    52741 cccgaaaccg tttaatgatc gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc
    52801 gcactggagc gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc
    52861 cttagtgctg aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac
    52921 cggtactgag tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg
    52981 tgaacattta agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga
    53041 tatgcacatt tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt
    53101 taaaaccttg cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc
    53161 gcgcgcatct tcgccatctt ctggctgacg ctggcgctgg tgttgatgtt ggttttgatg
    53221 ttacccaagc tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg
    53281 ctgatgattg agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg
    53341 tggtggcggc gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta
    53401 ttggtgacca ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt
    53461 aactttattg gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg
    53521 gaactggtcg gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt
    53581 cgtccggcca gtagttctca atccgatttc attaacttac tgtttgaccg cccgctatta
    53641 ctgctgattg tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg
    53701 gcaaaaccgg cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc
    53761 cagcacccgg aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac
    53821 cagatggtca ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc
    53881 tctcacgagc tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt
    53941 cgtagcggtg aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc
    54001 atgatcaacg atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa
    54061 accatcaaag ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag
    54121 caaatgggca agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat
    54181 ccgaacgccc tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat
    54241 acgaagattg aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac
    54301 gatggtcctg gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc
    54361 gatgaagcac gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc
    54421 gccattcagc agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg
    54481 ctggtgattt ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa
    54541 ggcgtttacg ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt
    54601 atcaggccta cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt
    54661 gccgctcagt cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga
    54721 gagtaaacgg tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc
    54781 ctgcacggtc acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt
    54841 ccctggcgct atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc
    54901 tatatcgctg atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac
    54961 ctgaattaat gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag
    55021 accgtcggtt gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg
    55081 aaattcccgc acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg
    55141 cccgccgtga acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat
    55201 caactcacca tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg
    55261 aatcttgcct gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt
    55321 ccccattaga ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc
    55381 aggaactcaa tagcaaaccg gacgaacgtt agttcatccg gctgtgggga ttagactggc
    55441 cccctgaatc tccagacaac cagtat
//
LOCUS       DAEEUE010000016        53214 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.016, whole genome shotgun sequence.
ACCESSION   DAEEUE010000016 DAEEUE010000000
VERSION     DAEEUE010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53214
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.016"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(12..323)
                     /locus_tag="KLK84_001022"
     CDS             complement(12..323)
                     /locus_tag="KLK84_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HBE2008445.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            486..582
                     /gene="ffs"
                     /locus_tag="KLK84_001023"
     ncRNA           486..582
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="KLK84_001023"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            702..1055
                     /locus_tag="KLK84_001024"
     CDS             702..1055
                     /locus_tag="KLK84_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HBE2008446.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1097..2647)
                     /gene="pdeB"
                     /locus_tag="KLK84_001025"
     CDS             complement(1097..2647)
                     /gene="pdeB"
                     /locus_tag="KLK84_001025"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HBE2008447.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSHVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2811..3281)
                     /locus_tag="KLK84_001026"
     CDS             complement(2811..3281)
                     /locus_tag="KLK84_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HBE2008448.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3397..3948)
                     /gene="maa"
                     /locus_tag="KLK84_001027"
     CDS             complement(3397..3948)
                     /gene="maa"
                     /locus_tag="KLK84_001027"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HBE2008449.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILANLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(4120..4338)
                     /gene="hha"
                     /locus_tag="KLK84_001028"
     CDS             complement(4120..4338)
                     /gene="hha"
                     /locus_tag="KLK84_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HBE2008450.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4364..4738)
                     /gene="tomB"
                     /locus_tag="KLK84_001029"
     CDS             complement(4364..4738)
                     /gene="tomB"
                     /locus_tag="KLK84_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HBE2008451.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5284..8433)
                     /locus_tag="KLK84_001030"
     CDS             complement(5284..8433)
                     /locus_tag="KLK84_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HBE2008452.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRNNEDIEHSHTVDHH"
     gene            complement(8456..9649)
                     /gene="acrA"
                     /locus_tag="KLK84_001031"
     CDS             complement(8456..9649)
                     /gene="acrA"
                     /locus_tag="KLK84_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HBE2008453.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9791..10438
                     /gene="acrR"
                     /locus_tag="KLK84_001032"
     CDS             9791..10438
                     /gene="acrR"
                     /locus_tag="KLK84_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HBE2008454.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10566..13928
                     /gene="mscK"
                     /locus_tag="KLK84_001033"
     CDS             10566..13928
                     /gene="mscK"
                     /locus_tag="KLK84_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HBE2008455.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14140..14301)
                     /gene="rsmS"
                     /locus_tag="KLK84_001034"
     CDS             complement(14140..14301)
                     /gene="rsmS"
                     /locus_tag="KLK84_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HBE2008456.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14315..14842)
                     /gene="priC"
                     /locus_tag="KLK84_001035"
     CDS             complement(14315..14842)
                     /gene="priC"
                     /locus_tag="KLK84_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HBE2008457.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHRELEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14912..15289
                     /locus_tag="KLK84_001036"
     CDS             14912..15289
                     /locus_tag="KLK84_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HBE2008458.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15442..15993
                     /gene="apt"
                     /locus_tag="KLK84_001037"
     CDS             15442..15993
                     /gene="apt"
                     /locus_tag="KLK84_001037"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBE2008459.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16122..18047
                     /gene="dnaX"
                     /locus_tag="KLK84_001038"
     CDS             16122..18047
                     /gene="dnaX"
                     /locus_tag="KLK84_001038"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBE2008460.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17380..17444
                     /gene="dnaX"
                     /locus_tag="KLK84_001038"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18100..18429
                     /locus_tag="KLK84_001039"
     CDS             18100..18429
                     /locus_tag="KLK84_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBE2008461.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18429..19034
                     /gene="recR"
                     /locus_tag="KLK84_001040"
     CDS             18429..19034
                     /gene="recR"
                     /locus_tag="KLK84_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBE2008462.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19144..21018
                     /gene="htpG"
                     /locus_tag="KLK84_001041"
     CDS             19144..21018
                     /gene="htpG"
                     /locus_tag="KLK84_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBE2008463.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21199..21843
                     /gene="adk"
                     /locus_tag="KLK84_001042"
     CDS             21199..21843
                     /gene="adk"
                     /locus_tag="KLK84_001042"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBE2008464.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21975..22937
                     /gene="hemH"
                     /locus_tag="KLK84_001043"
     CDS             21975..22937
                     /gene="hemH"
                     /locus_tag="KLK84_001043"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HBE2008465.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22934..23893)
                     /gene="aes"
                     /locus_tag="KLK84_001044"
     CDS             complement(22934..23893)
                     /gene="aes"
                     /locus_tag="KLK84_001044"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HBE2008466.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGINYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            24045..25349
                     /gene="gsk"
                     /locus_tag="KLK84_001045"
     CDS             24045..25349
                     /gene="gsk"
                     /locus_tag="KLK84_001045"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HBE2008467.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25482..27158)
                     /locus_tag="KLK84_001046"
     CDS             complement(25482..27158)
                     /locus_tag="KLK84_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HBE2008468.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27396..28616)
                     /gene="fsr"
                     /locus_tag="KLK84_001047"
     CDS             complement(27396..28616)
                     /gene="fsr"
                     /locus_tag="KLK84_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HBE2008469.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28834..30486
                     /gene="ushA"
                     /locus_tag="KLK84_001048"
     CDS             28834..30486
                     /gene="ushA"
                     /locus_tag="KLK84_001048"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HBE2008470.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30523..31002)
                     /gene="ybaK"
                     /locus_tag="KLK84_001049"
     CDS             complement(30523..31002)
                     /gene="ybaK"
                     /locus_tag="KLK84_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HBE2008471.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31206..32000)
                     /locus_tag="KLK84_001050"
     CDS             complement(31206..32000)
                     /locus_tag="KLK84_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HBE2008472.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32138..32479
                     /locus_tag="KLK84_001051"
     CDS             32138..32479
                     /locus_tag="KLK84_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBE2008473.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32694..35198)
                     /gene="copA"
                     /locus_tag="KLK84_001052"
     CDS             complement(32694..35198)
                     /gene="copA"
                     /locus_tag="KLK84_001052"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HBE2008474.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35460..36392
                     /gene="glsA"
                     /locus_tag="KLK84_001053"
     CDS             35460..36392
                     /gene="glsA"
                     /locus_tag="KLK84_001053"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBE2008475.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36395..37687
                     /locus_tag="KLK84_001054"
     CDS             36395..37687
                     /locus_tag="KLK84_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBE2008476.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37812..38246
                     /gene="cueR"
                     /locus_tag="KLK84_001055"
     CDS             37812..38246
                     /gene="cueR"
                     /locus_tag="KLK84_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HBE2008477.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAGGLRRDGSDM"
     gene            complement(38220..38678)
                     /locus_tag="KLK84_001056"
     CDS             complement(38220..38678)
                     /locus_tag="KLK84_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBE2008478.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38675..39592)
                     /locus_tag="KLK84_001057"
     CDS             complement(38675..39592)
                     /locus_tag="KLK84_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBE2008479.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39738..40415
                     /gene="fetA"
                     /locus_tag="KLK84_001058"
     CDS             39738..40415
                     /gene="fetA"
                     /locus_tag="KLK84_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HBE2008480.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40402..41181
                     /gene="fetB"
                     /locus_tag="KLK84_001059"
     CDS             40402..41181
                     /gene="fetB"
                     /locus_tag="KLK84_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HBE2008481.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41244..42098)
                     /gene="cnoX"
                     /locus_tag="KLK84_001060"
     CDS             complement(41244..42098)
                     /gene="cnoX"
                     /locus_tag="KLK84_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HBE2008482.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLQDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42159..42968)
                     /gene="ybbO"
                     /locus_tag="KLK84_001061"
     CDS             complement(42159..42968)
                     /gene="ybbO"
                     /locus_tag="KLK84_001061"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBE2008483.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42958..43580)
                     /gene="tesA"
                     /locus_tag="KLK84_001062"
                     /pseudo
     CDS             complement(42958..43580)
                     /gene="tesA"
                     /locus_tag="KLK84_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43551..44237
                     /locus_tag="KLK84_001063"
     CDS             43551..44237
                     /locus_tag="KLK84_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE2008484.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44234..46649
                     /locus_tag="KLK84_001064"
                     /pseudo
     CDS             44234..46649
                     /locus_tag="KLK84_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(47361..48455)
                     /gene="mnmH"
                     /locus_tag="KLK84_001065"
     CDS             complement(47361..48455)
                     /gene="mnmH"
                     /locus_tag="KLK84_001065"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBE2008485.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48524..49450)
                     /gene="allS"
                     /locus_tag="KLK84_001066"
     CDS             complement(48524..49450)
                     /gene="allS"
                     /locus_tag="KLK84_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HBE2008486.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVTPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49680..50162
                     /gene="allA"
                     /locus_tag="KLK84_001067"
     CDS             49680..50162
                     /gene="allA"
                     /locus_tag="KLK84_001067"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HBE2008487.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50240..51055
                     /gene="allR"
                     /locus_tag="KLK84_001068"
     CDS             50240..51055
                     /gene="allR"
                     /locus_tag="KLK84_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HBE2008488.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51145..52926
                     /gene="gcl"
                     /locus_tag="KLK84_001069"
     CDS             51145..52926
                     /gene="gcl"
                     /locus_tag="KLK84_001069"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HBE2008489.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52939..>53214
                     /locus_tag="KLK84_001070"
     CDS             52939..>53214
                     /locus_tag="KLK84_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HBE2008490.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIR"
BASE COUNT        12828 a        13691 c        14023 g        12672 t
ORIGIN      
        1 cccggagagg gttagtagtt ccagcgataa cgctgcaagt cgatttgccc gcttcccgat
       61 accatcacac cttctgccag taatgcctgt cgctgacgct gtaaatccgg cccggttagc
      121 gaaattgtgc catggcgatt aaccacccgg tgccagggta aggtgctgcc ttcagggaga
      181 cgctttaaca caccgcccac ctggcgcgcg gcgcggggcg atcccgccag tttcgccaca
      241 tcaccgtaag tggtgacata gccttcggga atagcggcga cgatttgcca gacgcgttgg
      301 ggaaatgaat cttctttttc catcttttct tcctgaggta atttttcagc ataatctgga
      361 aaaacgcccg agtgaagtcg cattgcgcaa gaaaccagca tctggcacgc aatgggttgc
      421 aattagccgg ggcagcagtg ataatgcgcc tgcgcgttgg ttttcaacgc tctcaatggg
      481 ggctctgttg gttctcccgc aatgctactc tgtttaccag gtcaggtccg gaaggaagca
      541 gccaaggcag atgacgcgtg tgccgggatg tagctggcag ggcccccacc catttctgcc
      601 tcccaccgtt tcgtcaaaaa atcccagcat ggctaaactt taaccacaac tgacgtcgca
      661 agaattgtct ggctgcgcag tacgcttcgg aggtatgtct gatgaagtat gttgatggtt
      721 ttgtggttgc cgttcctgcc gataaaaagg atgcctatcg ggaaatggcc gctaaggccg
      781 cgccattgtt taaagagttt ggcgcgcttc gtattgtcga atgctgggcc agcgatgtac
      841 cggatggcaa agtgaccgat tttcgtatgg cggtgaaagc ggaagagaat gaagaggttg
      901 tctttagctg gattgaatac ccttcaaaag aggtccgcga cgcagctaat caaaagatga
      961 tgtcggaccc acggatgaaa gagttcggcg agtccatgcc gtttgacggc aagcgaatga
     1021 tctatggcgg attcgagtca atcatcgacg aataggtagc gtgacgggct gtgcggcgtg
     1081 ccgtcagccc aaaaattcac aaatgttgct cggcccagcg taagaaatct tctttcggta
     1141 atgccttgct gtagagccag ccctgaccgt agtgcacgcc atgctggcgt agccactctt
     1201 cttgtttact ggtttcgatt ccctccgcta ccattttcag tttcagtgtt tttgccattt
     1261 cgatgatatg cggcgtgaca tttttatatt ccagcgcatc aacgaaagat ttatcgatct
     1321 tcagaatgtc gacatccaga tcctgtaaat aacttaaact tgaatacccc gtaccaaaat
     1381 catcaagata aatttcatgg cccgcctccc ggtagcgaga aattatcggg gcgctggttt
     1441 tcggatcggc aaactcgcgt tcagtgagtt caagcgcgat ctgtctggga ttaacctgat
     1501 agtgattgat catttcacgc agcaattgcg ggattttttc cgaggtgagc acggtggatt
     1561 caagattgat cgaaatatgc tgctgtggat gctgacgcaa gcagtcgccc atatcttcaa
     1621 agacgcttct tataatcagt agcgtcaatg gctcagaaag gcccgtttgc tgtgccagcg
     1681 gaataaaact atctggtgac aaccaactac cgtctgtctg cggccagcgc gccagtgcct
     1741 cagcaccgac aattttgcca ttggctaagg agacaatcgg ctgatagtgc acgcaaatat
     1801 cacgattttc gatagcatcc tgcagccgat gatgcggtga ttggatacgg cgcaaaatac
     1861 gcagcacaaa catcgctgcc agcaggccaa tcaccaaccc gagcggtaac caaataaaga
     1921 cttgccgatg ccagcctttc tgcaacattt tcgttgaagc ccaggtgatg atcgaaatat
     1981 tcatctccag gaagggcagt atatcgtaaa tgatcccatt attttcgata tgctcaccgg
     2041 gtgttttttg tagcctggta ataattccct gagcaatttc atcgctgctg gttatgacaa
     2101 cgttatgggc attgccaata atggcggcat caatttgcca tgagctatag ggaatgacat
     2161 caataaagga agcggggtcg atcatgacga cataatgtgc cgttcccatg gcgaccatgt
     2221 aacggataat gcctaaatcg ttatgcgatg ttaaccagac acgataacca tctttcgaaa
     2281 ttttacctag ctcgggaaag gtatcgggcg gactttcatg ctccagagac gaacactggg
     2341 gaacgttgtt atcgatataa accacttcct gaatatagcg ataactgtaa gatacccgac
     2401 gcatttccat gagatgggct tcgctacagg cagcgccttg ccatctttcc agctcctgca
     2461 gcgcatcttt cccttgtgtc gccaccttat tggctcgaat agcgacgtgg gaggaatagg
     2521 tatccagctc ttcaataaac gatgtttcta cctgctgatg ggccagccag atgcttaagc
     2581 cgacaggcag caatactgaa agaatcagta ctcccgaaat aaggccgacc agatgtcgtg
     2641 ttctcaccag catatcctta ttaacacagt gtagggaaag tatatctgat tgtggaaaaa
     2701 ggctggcagg agataaggag gtagagctgg tatttcatga agtgatgggt ggaaacgatc
     2761 attgtcattt gaccaggcgc agagctttac gcctggtatg taaccctaac ttaagatgtt
     2821 gattcggcgc gagcgagggt aaaaatatcg taaaaagaca gttcaccttt acggacgatc
     2881 attttttgca gttgttcctt atgaacatcc gcgacacgcg gagagttcaa gatggcttca
     2941 accagcgcgg caggtacaaa gcgcgtgttg taccattcgc cgttatagca aacgcggaaa
     3001 tccagcacgt cgatatcttc atagcggtag cgtgggtaga catcgaaaaa gaagaaacca
     3061 ttaagcagga taaataatac aaccagtagc cagacagagc ctgacagcgt ttcggactgc
     3121 aacatcaccg ccagggtggc aaaaaaagca acgtacatac cgataaacag ccccggatgt
     3181 ttacggataa aactgatact aaagcggggt ttgttgtcgc gtttttcgcg ggtattcaga
     3241 gactcaatcg tttcggtcag caggcgttgt atttcggtca tcgtttgcct tcgtggtatc
     3301 tgcaactttc cagggaacac cctattttaa cgtgggaata gtagagggga agtaacagat
     3361 ctaccacaat tttgcataac agttgcgaaa aaccgattac aattttttaa ttattctggc
     3421 tggattaccg cccacgacaa cgttgtccgg gacacctttt gtgacaactg cacctgaagc
     3481 taccacgacg ttatcaccaa tggtcacacc agggttaatg accgcgcgtc cgccaatcca
     3541 gacgttatta ccgatggtga cgggtttccc cagttcagca ccgctattac gtgctacagg
     3601 gtcgatggga tgtgttgccg tgtagatatg aacgcctggt gccaacatac agttatcacc
     3661 gatgcgaata gggcagacat caagcatcac gcaatcgaag ttggcgaaaa aattattgcc
     3721 gagaaaaatg ttatagccat aatcacagcg aaacgttggc tcaatataag cctctgtcac
     3781 ctgaccgaat aaattagcga gaatttgctg gcgtaatgtg tgctcttccg ccagggaatg
     3841 attgtatcgg tgaataagct gacgagcgcg caggcgatcg cgagataacg tctcatctgc
     3901 cgagcgatac aactcaccag caatcatctt ttctttttct gtgctcatta ctgacctccg
     3961 gttgctgaaa atgcggcaac acagtaagac gaaaagtcag tgaagagaac gttctcatta
     4021 catctgtgtg ataaagatca catagggtat gtcttcatgg cgaaaaagta taaaattctt
     4081 aataaacagc cggttatagc accgaaagcg aattatttat tagcgaataa atttccatac
     4141 tgaggaaggg atcttgtcgt acagtttatt catggtcaat tcggcgaggc ggtgatctgc
     4201 ggctgagtaa aataccgcca gttcattatc tgataattcg tatttatttt tctcgataac
     4261 acgctccagc gtgtcaattg tctggcaacg acgtaaacgc attaaataat cggtttttgt
     4321 taaaggtttt tcggacatac ttctacccat ggttgtaata attctaacaa gataaactcg
     4381 caggattctc tttcgtcgcg ttgacaaaac aacggaatag tcgattacct gacttccgcc
     4441 atttctgaag agcctgcata ttaataccat aactgctgaa caacataaag gtgtcatcca
     4501 gatattcgtc gatctgctca atgagcttat tgtcttcatt atacttaatt ttgtaattaa
     4561 gtgcgaaggt cgcaatatgc tcaatcagtt cattcaactg gaggttgatc gccgaggttg
     4621 ggtcgttaac ccaaccatga ttgctttctt caaggtttgc aagacagtca tgatacaggg
     4681 tttcacagag aaacttaagc tgtgcgatat catgtctttt gggtgagtat tcatccataa
     4741 cgcgtcccct tcttagtggt tgaactaacg gacacctttc gggatggaaa aaacttactg
     4801 acctggactt gccctctcgt ttgttagctt aactataagc cactctttgc aggttttcat
     4861 cgcattatta cgaaacatta caattaggaa ggaatcggga caggactcac tttttcatac
     4921 tagcactaac ccgcagcagg taaaagcagt ttgctcgaac tgttcataac atttcaggat
     4981 tatcgccaga tgaaattggt gacactaata ccaggattgc tctgaatatg acgtaataac
     5041 cgaggaatga ataaagaatt accgcaaata attaagaata gcctctaaat gattatggat
     5101 tattaaatct attagcgcta cttaatataa attaactaac aatcagtatt ttttatgaat
     5161 tttatacgtg gttaatactg gttttcgtat gagatcctga gttggtggtt caattactcc
     5221 ttaatgttcg taggttatgc ataaaaaagg ccgcttacgc ggccttagtg attacatgtt
     5281 gtatcaatga tgatcgacag tatggctgtg ctcgatatct tcattattgc ggctaaagcg
     5341 gcggcgaacc accacaaaga ataccggaac gaagaagatt gccagtaccg ttgcggtcac
     5401 catcccgccc attacaccgg tacctactgc gttctgcgcg ccggaaccag caccagtact
     5461 gataaccagc ggcataacgc cgaggataaa cgccagcgag gtcatcagga tcggacgtaa
     5521 acgcatccgc accgcatcaa gcgtcgcttc aatcagacct ttaccttctt tatccatcaa
     5581 gtctttggcg aattcgacga taagtatcgc gttcttcgcc gacaacccaa tggttgtgag
     5641 cagtcctact tggaagtaaa cgtcattggt caggccacgg aaggtggcag ccagcaacgc
     5701 accgataacc cccagcggaa cgaccagcat aacggagaac ggagtcgacc agctctcgta
     5761 cagcgccgcc agacacagga atacgacaat caacgaaatc gcgtacagtg aaggtgcctg
     5821 gttgccggag agacgttcct gataggacat ccccgtccag tcatagccaa caccggtagg
     5881 cagtttgctc gccagttgtt ccatcagctc cattgcttca ccggtacttt tacccggtgc
     5941 cgcctggcct aagatttcca tggatggcag gccgttgtaa cgttccagac gcggcgaacc
     6001 gtactcccaa cgagaagagg agaacgccga gaatggcacc atctgaccat cagcagcacg
     6061 aacataccag tcgccgatat catccggcag catacggtat ttcgcttctg acatgacgta
     6121 aactttcttc acacgaccgc ggtcgataaa gtcgttcaca tagctaccgc cccatgcagc
     6181 gcccagagtg gtgttaatgt cgttgataga aacacccagc gcctgcgctt tttcctggtc
     6241 gatatcaatc ttaaactgcg gggtatcttc cagaccgttt ggacgtacgc tggtcagcat
     6301 atcagggtgc ttcgctgctt ctgcaagcag ctggttacgc gcctgagtca gtttttcgtg
     6361 accaaggcca gcctggtcaa tcagctcaaa gtcaaagccg gttgcagtac ccagttccac
     6421 gattgcgggc aggttaaagg cgaaaaccat cgcatctttg atttgcgaga aagcgcgtgt
     6481 tgcacgcatg gtaatcgctt caactttgtt ttcttcgccc ggacgatcgg cccagtcctt
     6541 caaggaaacg aacgcaatac cggtattctg accacgtccc gcaaagccga agccgttaac
     6601 ggcgaacacc gactcaacgt tgttcttttc tttggtcaga tagtaatgcg ttacctcatt
     6661 gagcactttc tgcgtacgtt cctgcgttgc acctgctggc agctgaacca tggtcataaa
     6721 tacgccctgg tcctcatctg gcaagaagga gcttggcaga cgcacgaaca gataggccat
     6781 gccgaccacg atgatcagat acagcactag gtaacgcccc gtactgcgca gaataccgcc
     6841 tacgctgtcg gtgtagtggt gcgtgctctt ctcgaacatg cggttcaacc agccgaagaa
     6901 gcctttttta ccttccccgt gatcgccttt ggcaatcggt ttcagcatgg tggcacaaag
     6961 agccggagtc aggatcaacg ctaccagtac cgacagcgcc attgctgaaa caatggtaat
     7021 agagaactga cgatagatag caccagtaga accgccaaag aaggccatcg gtacgaatac
     7081 cgccgacagt accatcgcga taccgaccag agcgccctga atctgcccca tcgacttacg
     7141 ggtagcttct tttggcggca aaccttcttc cgccataaca cgctccacgt tttctaccac
     7201 aacgatagcg tcatccacca acaagccgat ggcgagcacc atcccgaaca ttgttagcgt
     7261 gtttatcgag aagccaaagg cggcaaggac ggcaaaggtc ccgagcaata ccaccggtac
     7321 ggcaatggtc ggaatcaacg tcgcgcggaa gttctgcagg aacagataca taaccaggaa
     7381 cacgaggatg atcgcttcga ccagcgtttt aaccacttcg tgaatagaga ttttcacgaa
     7441 cggcgtggtg tcgtacgggt aaacaatttt cagacccgac gggaagaacg gttccatctt
     7501 cgccagttca gcacggattg ccgcagcggt atccagcgcg tttgcaccgg tcgccagctt
     7561 gatccccaga ccggaagcag gttggccgtt aaactctgcg atgatgtcgt agttctcacc
     7621 acccagctca atcttcgcca catcacgcag cagtacgcgg gaaccatcct gattcacttt
     7681 cagcaggatt ttgccgaact cttcagtaga ggtcagacgc gtctgagcaa taatagaggc
     7741 gttaagctgt tggcctttca ccggcggcgt accaccgagc tgacccgccg caacctgggc
     7801 gttctgcgct ttgatggcgg taatgacatc aaccggcgtt agctggaatt tgttcagctc
     7861 attcgggttc atccagatac gcatcgcgta ctgtgaaccg aacaactgaa catcacccac
     7921 gcccgacgta cggctgatgg catctttcat attcgccgct acgtagtcag agatatcctc
     7981 ctgcgtcatg gtgccatcgg tgttgataac gccgacaacc atcaggaagc tgctggatga
     8041 tttctcaacg ctcacccctt gctgctgaac ttcttgcggc agcaacggca tcgccagctg
     8101 cagtttgttc tgcacctgaa cctgcgcgat atccgcatca gtaccagact caaaggtcag
     8161 ggtgatctgc acggtacccg tggagtcact gttagaggac atgtacatca ggttatcgat
     8221 accgttcata ttctgttcga taacctgtgt caccgtgtcc tgcactgttt tcgcatcagc
     8281 gccggggtag gaggcggaga tcgttactgc cggcggtgca atcgtaggat attgcgccac
     8341 cggcagtttg aggatcgcca gcccccctgc caacatgatg ataatggcga tcacccacgc
     8401 aaaaatcggg cgatcgataa agaaattagg catgtcttaa cggctcctgt ttaagttaag
     8461 acttggactg ttcaggctga gcaccgcttg cggcttgctg gttattatca gcggtaactt
     8521 cttgtgcttt tacctggaca ccaggacgca ctttctgcag cccacttatt actacgcgat
     8581 cgcctgcttt cagaccttct gtcaccagcc acttatcgcc aatagcctgg cttgcaacga
     8641 tcggacgggt ttccactttg tcatccgcgc caactaccag tacggtggca tcaccacgcg
     8701 gcgtacgggt tacgccctgt tgcgggacta aaatagcgtt tggattaagc ccttcttcca
     8761 gacgtgcgcg cacgaacata cccggcagca gagtgtgatc cgggttcggg aagatagcgc
     8821 gtagggtgat agacccagtg gtctgatcaa cggtaacgtc agagaattcc agcgtaccgt
     8881 cctgcgggaa cttaatgccg tcactggtga tcagcgacac tttggctttg ccgttctctt
     8941 gtttcagcgt gccattcgcc agttcctgtt tcaggcgaag gaagtcgttg ctggactggg
     9001 tcacatcaac gtagatcgga tcaagttgct gcacggttgc cagcgcagtc gcctgaccgt
     9061 tctgtaccaa tgcgccttcc gtcacgttcg acttaccaat gcgaccgcta atcggagagg
     9121 tgactttggt gtaagccaga ttgatccgcg cagtttcaac ggcagctttc gccgcagtta
     9181 ccgcagcatt cgcctgttgc gcatcagcca gagcctgatc gtacccttgc ttactgatgt
     9241 actgagtacc gagcagtttc tgataacgat tcaccgtcaa ttgcgcgata ttggctgcag
     9301 cctgggcttt cgccagatca cctttcgcac tgtcgtatgt cgcctgatag gtcgcaggat
     9361 caatctgata gagagagaca ccagcttcga tgtcgctacc ttctttgaaa ttacgcttca
     9421 ggataatccc gctaacttga ggacgaactt ctgcgatccg gtaggcactg gtgcgacccg
     9481 gaagctcggt tgtgatctgc agaggttcag ttttgactgt tactacgcca acggcgggca
     9541 tctgctggcc accttgttgg gcctgtttgt cgtcacatcc tgttagggct aagctgcctg
     9601 agagcatcag aacgaccgcc agaggcgtaa accctctgtt tttgttcata tgtaaacctc
     9661 gagtgtccga tttcaaattg gtcaatggtc aaaagttaat aaacccattg ctgcgtttat
     9721 attatcgtcg tgctatggta catacattca caaatgtatg taaatctaac gcctgtaaat
     9781 tcacgaacat atggcacgaa aaaccaaaca agaagcgcaa gaaacgcgcc aacacatcct
     9841 cgatgtggct ctacgtcttt tctcacagca gggggtatca tccacctcgc tgggcgagat
     9901 tgcaaaagca gctggcgtta cgcgcggtgc aatctactgg cattttaaag acaagtcgga
     9961 tttgttcagt gagatctggg aactgtcaga atccaatatt ggtgaactag agcttgagta
    10021 tcaggcaaaa ttccctggcg atccactctc agtattaaga gagatattaa ttcatgttct
    10081 tgaatccacg gtgacagaag aacgacgtcg attattgatg gagattatat tccacaaatg
    10141 cgaatttgtc ggagaaatgg ctgttgtgca acaggcacaa cgtaatctct gtctggaaag
    10201 ttatgaccgt atagaacaaa cgttaaaaca ttgtattgaa gcgaaaatgt tgcctgcgga
    10261 tttaatgacg cgtcgggcag caattattat gcgcggctat atttccggcc tgatggaaaa
    10321 ctggctcttt gccccgcaat cttttgatct taaaaaagaa gcccgcgatt acgtcgccat
    10381 cttactggag atgtatctcc tgtgccccac gcttcgtaat cctgccacta acgaataacc
    10441 ctgaatctga ctccaggatt tttcctggac attttcgtcg ttgctattct ggttcactgc
    10501 gtcgtgatat tcttgcggtt tgactttttc aggtcgttct tcaggttcag aaaccttcat
    10561 tcatcatgac tatgttccag tattacaaac gatcacggca ttttgttttt tcagcattta
    10621 ttgcttttgt ttttgtcttg ttatgccaga acacggcgtt tgcgcgggcg tcatcgaatg
    10681 gtgatctgcc gacaaaagcg gacctgcagg cgcaacttga ctcactaaat aaacaaaaag
    10741 atctttctgc tcaggacaaa ctggtgcagc aggatctgac agatacatta gccaccctcg
    10801 ataaaatcga tcgcataaaa gaagagacag ttcagctacg gcaaaaagtc gctgaagcgc
    10861 cggaaaaaat gcgccaggcg accgcggcgt taacagcact tagcgatgtc gataacgacg
    10921 aagaaacgcg caaaattctg agtacgcttt cgttgcgcca gctggaaact cgcgttgccc
    10981 aggcgctgga cgatttgcaa aacgcacaaa acgatctggc gtcttataac agccagctgg
    11041 tttcgttaca gacgcagccc gaacgcgtgc aaaatgcgat gtataacgct tcgcagcagc
    11101 tgcaacaaat tcgcagtcgt ctggatggga ctgatgtcgg cgagactgcc ttacgtccca
    11161 gccagaaagt gttaatgcag gctcagcagg cgttgctgaa tgcggagatt gaccagcagc
    11221 gtaaaagcct ggaagggaac accgtcttgc aggatacctt gcaaaagcaa cgtgattacg
    11281 tgacggcgaa cagcgcccgt ctggagcacc agttgcagct gttgcaagaa gcggtaaaca
    11341 gcaagcgcct gactttaact gaaaaaacgg cgcaggaagc cgtctccccg gatgaagccg
    11401 cgcgtattca ggctaatccg ctggtgaaac aggaactgga aattaaccag cagttaagtc
    11461 agcgtctgat taccgcgact gaaaacggta atcagttgat gcagcaaaac attaaagtca
    11521 aaaactggct ggagcgggcg ctgcaatcgg aacgcaatat taaagagcag attgccgtcc
    11581 tgaagggcag cctgctgttg tctcgtatcc tttaccagca acaacaaacg ctgccctcgg
    11641 cggatgaact ggaaaacatg accaaccgca tcgcggattt gcgtctcgaa cagtttgaag
    11701 ttaaccagca gcgtgatgca ctcttccaga gcgatgcgtt cgtcaacaaa ctggaagaag
    11761 gtcacaccaa cgaagtcaac agcgaagttc acgatgcgtt attgcaagtg gttgatatgc
    11821 gtcgcgaatt gctggatcaa ctcaacaaac agttgggtaa ccagctgatg atggccatta
    11881 acctgcaaat caaccagcag cagttaatga gcgtatcgaa aaacctgaaa tccatcctga
    11941 ctcagcaaat cttttgggtg aacagtaacc gtccaatgga ctgggactgg atcaaagcgt
    12001 tcccgcaaag cctgaaagat gaatttaagt cgatgaaaat cacggtgaac tgggaaaaag
    12061 cctggcctgc cgtttttatc gctttcctcg ccggtttgcc gctgctgttg attgccgggc
    12121 tgatccactg gcgtctgggc tggctgaaag cgtatcaaca gaaactggct tccgctgtgg
    12181 gttccctgcg taacgatagc cagctcaaca caccaaaagc gatccttatc gacctgatcc
    12241 gcgcgctgcc ggtgtgcctg attattctcg cggttggcct gattctgttg accatgcagc
    12301 tcaacatcag cgaactgcta tggtcgttca gtaaaaaact ggcgattttc tggctggtgt
    12361 ttggcctgtg ctggaaggta ctggagaaaa atggcgttgc cgtgcgtcac ttcggcatgc
    12421 cggagcagca gaccagccac tggcgtcggc aaattgtccg catcagtctc gcgttgctgc
    12481 ctatccattt ctggtctgtg gtggcagaac tttccccgct gcatctgatg gatgatgtgc
    12541 tggggcaggc gatgattttc ttcaacctgc tgctgattgc cttcctggta tggccaatgt
    12601 gccgcgaaag ctggcgtgat aaagagtcgc acaccatgcg actagtcacc attaccgtgc
    12661 tgtcgattat cccgattgca ttgatggtgc tgactgccac gggttatttc tacaccacgc
    12721 tgcgcctctc cgggcgttgg attgaaaccg tttatctggt gatcatctgg aacttgctct
    12781 accagacggt attgcgcggc ttaagcgtag cggcgcggcg tatcgcctgg cgtcgtgcgc
    12841 tggcgcgtcg gcagaatctg gtgaaagagg gcgcagaagg tgccgaaccg ccggaagaac
    12901 ccaccattgc actggagcaa gttaaccagc agacgctgcg tattaccatg ttgctgatgt
    12961 ttgcgctgtt cggtgtcatg ttctgggcaa tttggtccga tttgatcacc gtgttcagct
    13021 atctcgacag catcacgctc tggcattaca acggcactga agctggcgct gcggtggtga
    13081 aaaacgtcac catgggcagt ctgttgtttg cgattatcgc ctcaatggtg gcctgggcgt
    13141 tgattcgcaa cctgcctggt ttactggaag tgctggtgct ctcgcgactg aatatgcgcc
    13201 agggcgcgtc gtatgccatt actaccatcc ttaactacat cattattgcc gttggtgcga
    13261 tgacggtgtt cggatcgctg ggcgtctctt gggataaact ccagtggctg gccgcagcat
    13321 tatccgtagg tcttggtttt ggtttacaag aaattttcgg taacttcgtc tccggtttga
    13381 tcattctgtt cgaacgtccg gtgcgtattg gcgatacggt aaccattggt agcttctcgg
    13441 ggacggtaag taagatccgt attcgtgcga caacgattac cgatttcgat cgcaaagaag
    13501 tgatcatccc gaacaaagcg tttgttaccg agcgtctgat caactggtcg ttgactgaca
    13561 ctactacgcg tctggtgatc cgtctcggcg tggcctatgg ctccgatctg gaaaaagtgc
    13621 gtaaagtgtt actgaaggcg gcgactgagc acccaagggt gatgcacgaa ccaatgccgg
    13681 aagtcttctt tacggcattt ggtgccagca cgttggatca tgagctgcgt ctgtatgtgc
    13741 gtgaactgcg tgaccgtagc cgtactgtcg atgagctgaa ccgtactatc gatcagctgt
    13801 gccgtgaaaa cgacatcaac attgccttta accagcttga agtgcatctg cacaacgaga
    13861 agggcgatga ggtgacggaa gtaaaacgcg actacaaagg cgacgatccg acaccagcgg
    13921 ttgggtaata accaaagggg gcgacattta gttgccccga gattactgac aaagtgcgcg
    13981 ttgttcatgc cggatgcggc gtgaccgcct tatccggcct acgaaaccgc aagaattcaa
    14041 tatattgcag gagcggtgta ggcctgataa gcgtagcgca tcaggcagtt ttgcgtttgc
    14101 ccgcaatctt aggggacatt tagcggcccc atttatttct cacttttccg cctcatcatc
    14161 gtgcgttaat ttcttttcat aatcacgctt tacaatatcc agcgcgcgca gcacggtact
    14221 ggcagggatc tgattttcct ccagcagcac aatcaaatcg acagccagtt tgacatcgtc
    14281 aggggcattt tcaagtgaca tactctttcc attgctagcg ggttaagcgc gctagcctgt
    14341 tttcgatttt ttccagcgca tggcggcagc gtgccaggcg cgcttcatag gcttccaatt
    14401 cacgatgcag cgtttgctgt tccacgagat cggtcacccg cgccaggcgg gctctgcgtt
    14461 cggcaaccat ctcacgtagc cgccgctcaa aatcctgatg ctgaatacgt ttacgctgcc
    14521 agcgggaaat tttcggtggc gcactgtccc actcgcgcaa tgaccaggcg gaggcttcac
    14581 gcgcgatggc ttccagttgt gccgccagat gttccgccag ccaggccact tgcggcagct
    14641 gttgctgctc aactgcatga cgaagcgcag ccagattatc gcccgcctcg tcgagacaag
    14701 actgtagtgt tgtcgcacga gtctgaaaaa gatgcctgtc gaaacgagca cttagcgtgg
    14761 cgaactgtga caccggggca caacgctgac gcagcgtagc gagctgtcct tccagttttt
    14821 ccagcagcag ggcggttttc acgataagac ctcaatgaaa atgataattg ttatgctaaa
    14881 gtagccacta tgtaagctga caacactcaa tatgcaacga atcattttaa tcatcattgg
    14941 ctggctggcg gtagtgctgg gtacgctggg cgtggtatta ccggtattac cgacgacgcc
    15001 gtttatcctg ctggcggcct ggtgctttgc ccgttcttcc ccgcgctttc acgcctggtt
    15061 gctgtaccgc tcatggtttg gcagctatct acgtttctgg cagaaacatc atgcgatgcc
    15121 gcgcggcgtc aaaccgcggg cgattttgct tattttgctc acgtttgcca tttctctgtg
    15181 gttcgtccag atgccatggg tgcgcatcat gttgctggta attctcgcct gtttgctttt
    15241 ctatatgtgg cgaattccgg tgattgatga aaagcaagaa aagcactgaa gcacaacaat
    15301 cgcagttgca attattgcgt acagccagta cattctggcg ttttcgagca caggcgcagg
    15361 cggtcaaagg ttaaacaaca gttacttttg atacgtttaa aacgcgccgt gagtaccacc
    15421 gtaacaagca ggcatacact tatgaccgcg actgcacagc agcttgagta tctcaaaaat
    15481 agcatcaaaa gcattcagga ctacccaaaa cccggcattc ttttccgcga tgtcaccagc
    15541 ttactggaag acccgaaagc ttacgctctc agcatcgact tgctggttga gcgttacaaa
    15601 aatgcgggca ttaccaaagt tgtcggcacc gaagcgcgtg gcttcttgtt tggcgctccg
    15661 gtagctctgg gtctgggcgt tggctttgta ccggtccgta aaccgggcaa actgccgcgt
    15721 gaaaccatca gtgaaaatta cgacctggaa tacggcaccg atcagctgga aatccacgtt
    15781 gatgccatca aaccgggcga caaagttctg gtggtggacg atctgctggc aaccggcggc
    15841 actatcgaag cgaccgttaa actgatccgt cgtctgggtg gtgaagtggc tgacgctgcg
    15901 ttcattatca acctgtttga tctcggcggc gaacagcgtc tcgaaaaaca gggcattacc
    15961 agctacagcc ttgtcccgtt cccgggccat taattatcgc cagtcttgtg ctgcccacgc
    16021 tacggacagc acaagatgtg cattcagcct cgccgttctg acggggctgt gttagcatta
    16081 ccccttcgtg aatccacctt ccagcgtttc agagcctgcc aatgagttat caggttttag
    16141 cccgaaaatg gcgcccacaa acctttgctg acgtcgtcgg ccaggaacat gtgctgaccg
    16201 cactggcgaa cggcttgtcg ttagggcgta ttcatcatgc ttatcttttt tccggcaccc
    16261 gtggcgtcgg aaaaacctct atcgcccgac tgctggcgaa ggggctaaac tgcgaaaccg
    16321 gcattaccgc gacgccgtgc ggcgtgtgcg ataactgtcg tgaaatcgag caggggcgct
    16381 ttgtcgatct gattgaaatc gacgccgcct cgcgcaccaa agttgaagat acccgcgacc
    16441 tgctggataa cgtccagtac gctccggcgc gtggtcgttt caaagtttat ctgatcgacg
    16501 aagtgcatat gctgtcgcgc cacagcttta acgcactgtt aaaaaccctt gaagagccgc
    16561 cggagcacgt taagtttctg ctggcgacga ccgatccaca gaaattgccg gtgacgattt
    16621 tgtcacgctg tctgcaattt catctcaagg cgctggatgt cgagcaaatt cgccatcagc
    16681 ttgagcacat cctcaatgaa gaacatatcg ctcacgagcc gcgggcgctg caattgttgg
    16741 cgcgcgccgc tgaaggcagc ctgcgagatg ccttaagtct gaccgaccag gcgattgcca
    16801 gcggtgacgg ccaggtttca acccaggcgg tcagtgcgat gctgggtacg cttgacgacg
    16861 atcaggcgct gtcgctggtt gaagcgatgg tcgagggcaa cggcgagcgc gtaatggcac
    16921 tgattaatga agccgctgcc cgtggtatcg agtgggaagc gttgctggtg gaaatgctcg
    16981 gcttgttgca tcgtattgcg atggtacaac tttcgcctgc tgcacttggc aacgacatgg
    17041 ccgccatcga gctgcggatg cgtgaactgg cgcgcaccat accgccgacg gatattcagc
    17101 tttactatca gacgctgttg attggtcgca aagaattacc gtatgcgccg gaccgccgca
    17161 tgggcgttga gatgacgctg ctgcgcgcgc tggcgttcca tccgcgtatg ccgctgcctg
    17221 agccagaagt gccacgccag tcctttgcac ccgtcgcgcc aacggcagta atgacgccaa
    17281 ccttggtgcc gccgcaatca gcgccgcagc aggcaccgac tgtaccgctc ccggaaacca
    17341 ccagccaggt gctggcggcg cgccagcagt tgcagcgcgt gcagggagca accaaagcaa
    17401 aaaagagtga accggcagcc gctacccgcg cgcggccggt gaataacgct gcgctggaaa
    17461 gactggcttc ggtcaccgat cgcgttcagg cgcgtccggt gccatcggcg ctggaaaaag
    17521 cgccagccaa aaaagaagcg tatcgctgga aggcgaccac tccggtgatg cagcaaaaag
    17581 aagtggtcgc cacgccgaag gcgctgaaaa aagcgctgga acatgaaaaa acgccggaac
    17641 tggcggtgaa gctagcggca gaagccattg agcgcgaccc gtgggcggca caggtgagcc
    17701 aactttcgct accaaaactg gtcgaacagg tggcgttaaa tgcctggaaa gaggagagcg
    17761 acaacgcagt atgtctgcat ttgcgctcct ctcagcggca tttgaacaac cgcggtgcac
    17821 agcaaaaact ggctgaagcg ttgagcatgt taaaaggttc aacggttgaa ctgactatcg
    17881 ttgaagatga taatcccgcg gtgcgtacgc cgctggagtg gcgtcaggcg atatacgaag
    17941 aaaaacttgc gcaggcgcgc gagtccatta ttgcggataa taatattcag accctgcgtc
    18001 ggtttttcga tgcggagctg gatgaagaaa gtatccgccc catttgatcg taagcacagc
    18061 ttacgttcgt catccttaac gtgattgaga gagaaaccta tgtttggtaa aggcggtctg
    18121 ggtaacctga tgaagcaagc ccagcagatg caagaaaaaa tgcagaaaat gcaggaagag
    18181 atcgcgcagc tggaagttac cggcgaatct ggcgcaggtc tggtaaaagt gaccatcaac
    18241 ggtgcacaca actgccgtcg cgtagagatc gacccgagcc tgctggaaga cgacaaagag
    18301 atgctggaag acctggtggc tgcagcattc aacgacgcag cacgtcgtat tgaagaaacg
    18361 cagaaagaaa aaatggcctc tgtatcctcc ggaatgcagc tgccgcctgg ctttaagatg
    18421 ccgttctgat gcaaaccagc ccgctgttaa cacagcttat ggaagcactg cgctgtctgc
    18481 cgggcgttgg cccgaagtcg gcgcagcgta tggcgttcac gctgcttcag cgcgatcgta
    18541 gcggcgggat gcgtctggcg caggcgctca cccgggcgat gtcggaaatc ggccactgcg
    18601 ccgattgccg cactttcacc gaacaggaag tctgtaacat ctgttcgaat ccgcgtcgtc
    18661 aggaaaacgg tcaaatctgt gtggtggaga gtccggcgga catctacgcc attgagcaga
    18721 cggggcagtt ttctggtcgt tattttgtgt tgatggggca tctgtcaccg ctggacggca
    18781 tcggtccgga tgatatcggg cttgatcgtc tggaacagcg tctggcagag gaaaaaatca
    18841 ctgaagtgat cctcgccacc aatcccacgg ttgaaggtga agctaccgct aactacattg
    18901 ccgagctttg cgcgcaatat gacgtggaag ccagccgaat cgctcatggc gtaccggttg
    18961 gcggcgaact ggaaatggtc gatggcacca cgctgtcaca ctccctcgcc gggcgtcata
    19021 agattcgttt ttaagcaaac gagagcagga gcacctgctc tcgcttgaaa ttattctccc
    19081 ttgtccccat ctctccctca tcctgttttt aaccttaaaa tggcattatt gaggtagacc
    19141 tacatgaaag gacaagaaac tcgtggtttt cagtcagaag tgaaacagct tctgcacctg
    19201 atgatccatt ctctctattc caataaagaa atcttcctgc gtgagcttat ctctaacgcc
    19261 tccgatgcgg cggacaagct gcgtttccgt gcgctatcta acccggacct gtacgaaggt
    19321 gatggcgaac tgcgcgttcg tgtctctttc gataaagaca agcgcacgct gaccatctcc
    19381 gataacggcg tggggatgac ccgcgacgaa gtgattgacc atctggggac tatcgctaaa
    19441 tccggtacca aatcattcct cgaatccctg ggttctgacc aagcgaaaga cagccagctg
    19501 atcggtcagt ttggtgttgg tttctactct gcgtttatcg tggccgacaa agtgaccgtg
    19561 cgtactcgcg cggcaggcga aaaaccagaa aacggcgtct tctgggaatc ggctggcgaa
    19621 ggtgaatata ccgttgccga tatcaccaaa gaagatcgtg gtactgaaat caccctgcat
    19681 ctgcgtgaag gcgaagacga gttcctcgat gactggcgcg tgcgttccat catcagcaaa
    19741 tactccgacc atatcgcgct gccggtagag atcgaaaaac gcgaagagaa agatggcgaa
    19801 accgttatct cctgggcaaa aatcaacaaa gcgcaggcgc tgtggactcg taacaagtcg
    19861 gaaatcaccg atgaagagta taaagagttc tacaaacaca tcgcccacga ctttaacgat
    19921 ccgctgacct ggagccacaa ccgcgttgaa ggtaagcagg agtacaccag cctgctgtac
    19981 atcccgtccc aggctccgtg ggatatgtgg aaccgtgatc ataaacacgg cctgaaactg
    20041 tacgttcaac gcgtgtttat catggacgac gctgaacagt tcatgccgaa ctatctgcgc
    20101 ttcgtgcgtg gtctgattga ctccagcgat ctgccgctga acgtttcccg tgaaatcctc
    20161 caggacagca cggtaacgcg taacctgcgc aatgcgctga ccaagcgtgt gctgcaaatg
    20221 ctagaaaaac tggcgaaaga cgacgcggaa aaataccaga ccttctggca acagtttggc
    20281 ctggtactga aagaaggtcc ggcggaagat ttcgctaacc aggaagcgat cgccaaactg
    20341 ctgcgttttg cttctactta taccgattct tctgcgcaga ccgtgtctct ggaagactac
    20401 gtttcccgca tgaaagaagg gcaggagaaa atctactaca tcactgcaga cagctatgcg
    20461 gcagcgaaga gcagcccgca cctggaactg ctgcgtaaga aaggcatcga agttctgctg
    20521 ctttccgacc gcatcgatga gtggatgatg aactatctga ctgagttcga cggtaaaccg
    20581 ttccagtcgg tgtctaaagt tgacgagtcg ctggaaaaac tggctgacga agttgatgag
    20641 agcgcgaaag aagcggagaa agcactgact ccgttcatcg accgcgtgaa agctctgctc
    20701 ggcgagcgcg tgaaagatgt ccgtctgact caccgtctga ccgatacgcc agcgatcgtt
    20761 tcgaccgacg cggacgaaat gagcactcag atggcgaaac tgttcgctgc ggcgggccag
    20821 aaagtgccag aagtgaaata catcttcgaa ctgaacccgg atcacgtact ggtgaaacgt
    20881 gcggcagata ctgaagatga agccaagttc agcgagtggg tagaactgct gctggatcag
    20941 gcgctgctgg cagaacgcgg cacgctggaa gatccgaacc tgtttattcg tcgtatgaat
    21001 cagctgctgg tttcctgatg tcatgccgga tgacctttgt gtcatccggc atttttcttt
    21061 tcatcatctg tactttccgc aaattatctc gccattaacc gtttcagcct caggtgcctt
    21121 tcttgaggca atcgcctgtt ggtggtatcg tttatcgctt tttcaaaaaa atcgacacat
    21181 tttaagggga ttttcgcaat gcgtatcatt ctgcttggcg ctccgggcgc ggggaaaggg
    21241 actcaggctc agttcatcat ggagaaatat ggtattccgc aaatctccac tggcgatatg
    21301 ctgcgtgctg cggtcaaatc tggctccgag ctgggtaaac aagcaaaaga cattatggat
    21361 gctggcaaac tggtcaccga cgaactggtg atcgcgctgg ttaaagagcg cattgctcag
    21421 gaagactgcc gtaatggttt cctgttggac ggcttcccgc gtaccattcc gcaggcagac
    21481 gcgatgaaag aagcgggcat caatgttgat tacgttctgg aattcgacgt accggacgaa
    21541 ctgattgttg atcgtatcgt aggccgccgc gttcatgcgc cgtctggtcg tgtttatcac
    21601 gttaaattca atccgccgaa agtagaaggc aaagacgacg ttaccggtga agaactgact
    21661 acccgtaaag acgatcagga agaaaccgtg cgtaaacgtc tggttgaata ccatcagatg
    21721 actgcaccgc tgatcggcta ctactccaaa gaagcggaag cgggtaacac caaatacgcg
    21781 aaagttgacg gtaccaagca ggttgctgaa gttcgcgctg atctggaaaa aatcctcggc
    21841 taatcaagcc gcagcagatc tgctttcggg caggtctgct tatccacctc aggcaatacc
    21901 tctcacagca attagttctt cttcctcact tttccgctac aattatcaac aagttgaatc
    21961 gataagaggc ggtaatgcgt cagactaaaa ccggtatcct gctggcaaac ctgggtacgc
    22021 ccgatgcccc cacacctgaa gcggtaaaac gctatctgaa acaattttta agcgacagac
    22081 gcgtggttga tacctcacgg ttgttatggt ggccgttgct gcgcggcgtg attttgccgc
    22141 tgcgctcgcc gcgtgtggcg aagctgtacg cctctgtctg gatggaagac ggctcgccgc
    22201 tgatggttta cagccgccag caacagcagg cgttggcaca acgtttaccg gatacgccag
    22261 tggcgctggg catgagctac ggctcgccat cactggaaag ctccgtcgat gaactcctgg
    22321 cagagcatgt agatcatatt gtggtgctgc cgctttatcc gcaattctcc tgttctacgg
    22381 tcggtgcggt atgggatgaa ctggcacgca ttctggcgcg caaacgtagc attccgggga
    22441 tatcgtttat tcgtgattac gccgataacc acgattacat taatgcactg gcgaacagcg
    22501 tacgcgcttc ttttgccaaa catggcgaac cggatctgct gctgctctct tatcatggca
    22561 ttccccagcg ttatgcagat gaaggcgatg attacccgca acgttgccgc acaacgactc
    22621 gcgaactggc ttccgcactg gggatggtac cggaaaaagt gatgatgacc tttcagtcgc
    22681 gctttggccg ggaaccctgg ctgatgcctt ataccgacga aacgctgaaa atgctcggag
    22741 aaaaaggcgt aggtcatata caggtgatgt gcccgggctt tgctgcggat tgtctggaga
    22801 cgctggaaga gattgccgag caaaaccgtg aggtcttcct cggtgcgggc gggaaaaaat
    22861 atgaatatat tccagcgctt aatgccacgc cggaacatat cgaaatgatg gctaatcttg
    22921 ttgccgcgta tcgctaaagc tgagcggtaa agaactgagc gccgtcgcga agagcctcgt
    22981 cggcggtttt catcatccgt gaataatgca aaaaggcatg cagcgtacct gggtagagtt
    23041 tgaactcaca gggctgctga tgcgccgcta acgtctggta aagcagacgg ctgtcatcca
    23101 gcagcggatc gaactccgcc ccggcaataa aacagggcgg aacttcgcga gtgagatcat
    23161 tattaaacag acagtaatac ggtgactcgc ggtccgcgtc gttgcttaaa tacgcctctt
    23221 catacatctg caaatcctgt tgcgttaagc catcccagac accgcccaac agacgacgag
    23281 tcacggaatc ccgtaatccg taaagcccat accacagcaa aacacccgca attttaccgc
    23341 aatcgatctg tttatcacgc aaccacaacg cactggcgag cgccagcatg gcacctgcgg
    23401 aatcaccggc aaagccaatg cgggacatat tgatttgata atcctccgcc tgctggtgga
    23461 aataacaaca agcagccaca atttcctcta tcgcttgtgg aaaacgcgct tcaggtgaaa
    23521 gggtgtaatt aataccaatc accgtacatt ggctgtagct tgccagcagg cgcatgatgc
    23581 gatcgtgggt atcgagattg ccgagaataa aaccgcctcc atgcaaataa aatagcgtcg
    23641 ccgggctatc tggctgcgga caaaagagac gtgtttccac ctgcccatat tttgttggaa
    23701 ccatgtaagc tctggttgcc atttctggag cgcccgcatt ccagaatcgg cgctcaagcg
    23761 tgtaatactg tcgttgctca gcaatcgttc ccgttgcggg ccaggacggt aaatccggct
    23821 gaagagtatt cacaacggtc ttcatttcag cagaaataag gtccagaaca ggtagtttgt
    23881 tttccggctt catacaaaaa ctcctttcaa attacgtcat tgtaaggaac cactgccatg
    23941 aaaatgcgat cccgcctgct gatattgaaa ctggctgcgt ctcgcgcgct cccgtcagat
    24001 tgtgttaaca ttcgccgctc agttaaccac ccgtaaaaac aaccatgaaa tttcccggta
    24061 aacgtaaatc caaacattac ttccctgtaa atgcacgcga tccgctgctt cagcaattcc
    24121 agccagaaaa cgaaaccagc gccgcctggg tcgtaggtat tgaccagacg ctggtcgata
    24181 ttgaagcgaa agtggatgat gaattcattg agcgttatgg attaagcgcc gggcattcac
    24241 tggtgattga ggatgacgta gccgaagtgc tttatcagga gctaaaacag aaaaacctga
    24301 ttacccatca gtttgcgggt ggcaccattg gtaacaccat gcacaactac tcggtgctcg
    24361 cggacgaccg ttcggtgctg ctgggcgtca tgtgcagcaa tattgaaatt ggcagttatg
    24421 cctatcgtta cctgtgtaac acttccagcc gtaccgatct taactatcta caaggcgtgg
    24481 atggtccgat tggtcgttgc tttacgctga ttggcgagtc cggggaacgt acctttgcta
    24541 tcagtccagg ccacatgaac cagctgcggg ctgaaagcat tccggaagat gtgattgccg
    24601 gagcctcggc actggttctc acctcatatc tggtgcgttg caagccgggt gaacccatgc
    24661 cggaagcaac catgaaagcc attgagtacg cgaagaaata taacgtaccg gtggtgctga
    24721 cgctgggcac caagtttgtc attgccgaga atccgcagtg gtggcagcaa ttcctcaaag
    24781 accacgtttc tatccttgcg atgaacgaag atgaagccga agcgttgacc ggagaaagcg
    24841 atccgttgtt ggcatctgac aaggcgctgg actgggtaga gctggtgctg tgcaccgccg
    24901 ggccaatcgg cttgtatatg gcgggcttta ccgaagacga agcgaaacgt aaaacccagc
    24961 atccgctgct gccgggcgct atagcggaat tcaaccagta tgagtttagc cgcgccatgc
    25021 gccacaagga ttgccagaat ccgctgcgtg tctattcgca cattgcgccg tacatgggcg
    25081 ggccggaaaa aatcatgaac actaatggag cgggggatgg cgcattggca gcgttgctgc
    25141 atgacattac cgccaacagc taccatcgta gcaacgtacc aaactccagc aaacataaat
    25201 tcacctggtt aacttattca tcgttagcgc aggtgtgtaa atatgctaac cgtgtgagct
    25261 atcaggtact gaaccagcat tcacctcgtt taacgcgcgg cttgccggag cgtgaagaca
    25321 gccaggaaga gtcttactgg gatcgttaag ttatcgtcgg ttcgtaggcc agataaggcg
    25381 ttcacgccgc atctggcatt tggctctcga tgcctgatgc gacgctggcg cgtcttatca
    25441 tgcctacata tttttcatat tttacatccg gcaaccaccg tttaccccgt caccacttca
    25501 cccgccggtg gcgtttccag cagttccagc atagtacggg caatttcacg ttcacccatc
    25561 actacctgat tcgcgccacg ttcggtgata tacgtcactt catcgtcata atgggcgcgg
    25621 gcgataatct caatatccgg gtttttcgcg cgggcagacg ccacaatctc acccgcttca
    25681 taaccgttgg ggatcgtcag gatcagccat tttgcacatt ccagatgtgc cagttgcata
    25741 atttcttcgt tcgctgcatt gcccaataca gcgcggaccc cacgctctcg cagctcatca
    25801 acacgggttc gtgacgtctc aatcaccacc agcggaatat cagaggcgag caatttctcc
    25861 cccagcaggc tgcctacacg accgtaaccc accaatagcg catggttgca aatatccact
    25921 gggatctgtt tctcttcttc gattgcctct tccagcgtct gctcttccag cgtttcggtc
    25981 ttcgccagat atttctccag tagtgcgaac agtaccgggt tgagcataat cgacaggatc
    26041 gcccctgcca gtaccaggtt ttgtccggcc tgcggcagta aattcaatgc cattcccagt
    26101 cccgccagga taaacgcgaa ctcaccaatc tgcgccaggc tggcggcgat ggttaatgcc
    26161 gtacgttggg agtgaccaaa cagacggaca aggaacaggg ccgcgaccga tttaccaaac
    26221 agaataattg ccagcgtcgc cagcactgcc agcggttgct gaatcagaat taacggatca
    26281 aacaacatcc cgactgagac aaaaaacagc accgcaaacg cgtcgcgcaa tggcaacgta
    26341 tcgtgggcgg cacggtgact caattcagac tcgttcagta ccatcccggc aaagaacgca
    26401 ccgagtgcaa aggagacatc aaacagctct accgcaccaa aggcaatccc taacgccagc
    26461 gccagcactg acagggtaaa cagctcgcga gaaccggttg ccgcgctgcg tgccataatc
    26521 cacggcacca gacggcgacc taccagcatc ataatggcga taaatgcgat caccttgccg
    26581 atggtgatcc ccatatcgac tgcaagagtg gcaaagccca catcgccctg ttccatcatt
    26641 cctgccactg cgggcagcaa caccagcgtc agaaccatca ccaggtcttc cacaatcaac
    26701 caaccgatgg cgatttgccc acgctgactg tctattaatt gccgttcttc aagtgcgcgc
    26761 agtaacacca cggtactggc ggtggaaaga cataagccga acacgatacc ggtcattaac
    26821 gaccagccca gcacggcgga gagcgccata cccagcagcg tcgccacggc tatctgggcg
    26881 atcgcgccgg gaatggcgat ggactttacc gccatcaaat ccttgagcga aaaatgcaaa
    26941 ccgacgccga acatcagcaa aataacgccc agttcagcca gttccggcgc aagcttggta
    27001 tcggcaacaa agcccggagt gaatggtcct gccagcacac ccgctaacag atatcccacc
    27061 agaggagaaa tacgtagttt attggccagc atgccgagga taaaggcgag cacaaggccg
    27121 ccaacaatgg tggtgataag cggggtggcg tgatgcattc cgtctccttt tcctggtggt
    27181 tattgtccat ttttggctgg gaaaaccaaa attacaggta atagtttatg acaatttcat
    27241 tgatgatgtt tatgaataat tgttgaattt tgcagaaaaa tggaattagc tgcaaaaaaa
    27301 gcacggttcg gaaaacagaa ggcggtttaa cagaggaaag gcttatggcg actgtaaaac
    27361 gatgcagcca aagtttgcct ttggctgcaa tgaaatcagt ctttatgccg gttatcaggc
    27421 aggaatatgg tcaacatccc caatagtggc aggaaagcac agattttata gactaactcg
    27481 atgctggtgt gatcggcgat aagccccaga actgccgctc ccagacctcc catgccgaaa
    27541 gcaaaaccga aaaagagtcc agaaaccata ccgatacgtc ccggaagtag ctcctgagcg
    27601 tagaccagaa tggcagagaa tgccgaagcg aggataaatc caataatcac cgttaaaacc
    27661 cccgtccagt gcaggcgggc gtagggtaaa atcagcgtaa acggcgcaac gccgaggata
    27721 gagccccaaa tcacatattt ccgtccaatt ttatccccta caggcccgcc gatcaccgta
    27781 cctgccgcaa cggcaaacag gaaggcaaac agatgaagct gggcattctg gatagataat
    27841 ccgaattttt gcatcagata aaaggtgtaa tagctgctga tgctcgccat atagaaatat
    27901 ttcgagaaaa tgaggattaa cagaatgctg actgccagta ccactttgtt gcgcggcagt
    27961 ggattgatta tcgtcgcttt gggttttcct ttattcattc ggtgctgtgc cgagtaccaa
    28021 cggctaattt gcgccaacac cacgatcgcc agcagtgccg caagcacaaa ccaggcaacg
    28081 ttgcctttgc cataaggcgc gataatcacc gccgccagca agggtcccag ggaactgcca
    28141 aagttgccgc cgacctgaaa gatagattgc gccaggccat gccgcccgcc ggaagccata
    28201 cgggctacgc gagaagattc cggatgaaag accgatgaac cggtaccgac cagcgccgct
    28261 gccagcagaa ctgcgccaaa actgcccgcc agcgcaagca gcaccagacc acttaaggta
    28321 aagcacatgc caattggcaa cgaccacggc atcggatatt tatcggtcca gtagccgacc
    28381 actggttgca gtagcgaaga ggcgagctgg aaggtgaggg ttatcatgcc aatctgcata
    28441 aatgtcagag aaaattctga ctgaagcagc ggataaatcg ccagaatcag cgattggatc
    28501 atgtcgttca gcagatgtga gaggctgata gcacctaaaa taccaaacga tgttcgggcc
    28561 ttggtcgttg acgcagccgc gcccgccaca ggctgggttt gttcactcat tgccatagga
    28621 aagtcacttt ttcagggttg cgatgtaaag aatgatctta tttgtgatta ttacccgact
    28681 aacatacctg tatgcgtcgt ctgaaggaag tctcaacgcc gaatacagaa tttctaatct
    28741 ggatgcagat ttatcttcac cggacgcaga cttgtctatg atgtcgcgtc atactatttt
    28801 tcaacacgtt gaaatcaggt cagggagaga agtatgaaat tattgcagcg gggcgtggcg
    28861 ttagcgctgt taaccacatt tacattggcg agtgaaactg ctctggcgta tgagcaggat
    28921 aaaacctaca aaattacagt tctgcatacc aatgatcatc atgggcattt ttggcgcaat
    28981 gaatatggcg aatatggtct ggcggcgcaa aaaacgctgg tggatggtat ccgcaaagag
    29041 gttgcggctg aaggcggtag cgtgctgcta ctttccggtg gcgacattaa cactggcgtg
    29101 cccgagtctg acttacagga tgccgaacct gattttcgcg gtatgaatct ggtgggctat
    29161 gacgcgatgg cgatcggtaa tcatgaattt gataatccgc tcaccgtatt acgccagcag
    29221 gaaaagtggg ccaagttccc gttgctttcc gcgaatatct accagaaaag tactggcgag
    29281 cgcctgttta aaccgtgggc gctgtttaag cgtcaggatc tgaaaattgc cgttattggg
    29341 ctgacaaccg atgacacagc aaaaattggt aacctggaat acttcactga tatcgaattt
    29401 cgtaagcccg ccgatgaagc gaagctggtg attcaggagc tgcaacagac agaaaagcca
    29461 gacattatta tcgcggcgac ccatatgggg cattacgata atggtgagca cggctctaac
    29521 gcaccgggcg atgtggagat ggcacgcgcg ctgcctgccg gatcgctggc gatgatcgtc
    29581 ggtggtcact cgcaagatcc ggtctgcatg gcggcagaaa acaaaaaaca ggtcgattac
    29641 gtgccgggta cgccatgcaa accagatcaa caaaacggca tctggattgt gcaggcgcat
    29701 gagtggggca aatacgtggg acgggctgat tttgagtttc gtaatggcga aatgaaaatg
    29761 gttaactacc agctgattcc ggtgaacctg aagaagaaag tgacctggga agacgggaaa
    29821 agcgagcgcg tgctttacac tcctgaaatc gctgaaaacc agcaaatgat ctcgctgtta
    29881 tcaccgttcc agaacaaagg caaagcgcag ctggaagtga aaataggcaa aaccaatggt
    29941 cgtctggaag gcgatcgtga caaagtgcgt tttgtacaga ccaatatggg gcggttgatt
    30001 ctggcagccc aaatggatcg cactggtgcc gactttgcgg tgatgagcgg aggcggaatt
    30061 cgtgattcta tcgaagcagg cgatatcagc tataaaaacg tgctgaaagt gcagccattc
    30121 ggcaatgtgg tggtgtatgc cgacatgacc ggtaaagagg tgattgatta cctgaccgcc
    30181 gtcgcgcaga tgaagccaga ttcaggtgcc tacccgcaat ttgccaacgt tagctttgtg
    30241 gcgaaagacg gcaaactgaa cgaccttaaa atcaaaggcg aaccggtcga tccggcgaaa
    30301 acttaccgta tggcgacatt aaacttcaat gccaccggcg gtgatggata tccgcgcctt
    30361 gataacaaac cgggctatgt gaataccggc tttattgatg ccgaagtgct gaaagcgtat
    30421 atccagaaaa gctcgccgct ggatgtgagt gtttatgaac cgaaaggtga ggtgagctgg
    30481 cagtaatccg aaagtgccgg atgtttgcat ccggcacaat gcttaatcgc ggcgggcgat
    30541 atcagcaaat ttggcatcga ggatctttgc cagatcgcct gccgccagtt cgatatccag
    30601 tccgcgcttg ccgccggaaa cataaatagt ggcaaattct tgtgcggggg cgtcgataat
    30661 cgttggcaga cgttttttct gccccagtgg gctaatcccc ccaaccaggt atcccgtcga
    30721 acgctgcgcg accatcggat cggccatctc aactttcttg gcacccagcg cttttgctac
    30781 ttttttaaga tccagttgac ctgcgaccgg cgtaacggcc acggcaaggt gtttcatatc
    30841 accgttcact gccaccagca gcgttttgta gacctgatcc ggattcaaac ctaatttttt
    30901 gacgacttca tcgccaaaat tggtttcagc cggatcgtgc tcgtaggtat ggatttgaaa
    30961 cgaaatcttg tttttttcga gtaatttaac tgcgggtgtc atggtaattt tcctgcctta
    31021 aacaaaaaaa cgaagcaagc atacgcctga tggcggtcca aaaaaaagag tcatcttgcc
    31081 taagagtatt ggcaggatgg tgagattgag cgacaatcga gttacaccgt cgcttaaagt
    31141 gacggcataa taataaaaaa atgaaattcc tctttgacgg gccaatagcg atattggcca
    31201 tttttttagc gcaacatttg cggcaaattc ccttctccat acaggtgtag tgcaccgacc
    31261 gcgaccacat atcgccccgg cggcatggcg cgtaatttat cccgccaggc gagatttcgc
    31321 tgatgcatca gcacatcgta cagcgactga ctgaacgtat tgggcagcgt tatatcatta
    31381 ttttgcggcg gtgcattcag ccaccagctc atcatttgtt gcagcaaccg tgcgttggta
    31441 tgccagtggg tcagcgtatc gtccagcagc gccagtcctt tgtcagggag ctggagcaac
    31501 atggcaatct ggttttcagc cccttccagt tcaatcacgg gtttatgttg ttgcttcgcc
    31561 gcctgcaata gctggtaatc gataccgtat tctgcccgca gccccagttt ttgtgcctgc
    31621 gtcgcctgaa gaaccatcgc gatttgccac agcggttggg tagaaaagag tgagggagaa
    31681 atgcccatct cctggctaat gtgctgcagg ttttggagtt gctcctcgct aatgcgctct
    31741 tccagcgcct cgcaggcagg caaattagca aaaggcgtat cgctggtgga aacatccgcc
    31801 tcgacgatca gcgcatcggc gtttttgagc tttttgagca aacgggtggg caggggagcc
    31861 atatcgtggc tacccatatg aatactgcca atcagatgaa aatggcgatt gccggggagg
    31921 gtgatatcga tggcaggcca ggtgtaatga ttaccgcgca gcgcggccca aagcgttttt
    31981 acccggtaca acagatccat acgacctccc tttgtgaaat atcatgctag cgcgcggtga
    32041 gggatggcgc aaatggagtt ggggacttgt ttaatgtgtt tgtatgattc agtatgttct
    32101 tgcatcgcta ttcacaggga agcaacagtt aaaaaccatg aaacaggcaa caagaaaacc
    32161 gacgacaccc ggagatattc ttctctatga atatctggaa ccgctcgatt tgaaaatcaa
    32221 tgagttagca gagttgctgc atgttcatcg taatcgcgtc agtgcactga tcaataacaa
    32281 tcgtaaactc accactgaga tggcattccg tctggcgaaa gtttttgata ccacagtcga
    32341 ttttaggcta aacctccagg cggcagttga tctttgggaa gttgaaaaca acatgcgcac
    32401 ccaggaagaa ttgggacgga ttgaaacagt ggctgaatat ttggcacgcc gtgaagagcg
    32461 tgcaaaaaag gtcgcgtaag gccaaaacat cgcttcatct cgtaggccgg ataaggcgtt
    32521 cacgccgcat ccggcatccg agcaccattg cctgatgcga cgcttacgcg tcttatcagg
    32581 cctacaaacc tgtgcctgaa ccgtaggccg gataaggcgt ttacgccgca tccggcagtc
    32641 atgcgtcgat gccaaatgcg ccgccctaaa gcagcgcatc cgcaatgacg tacttattcc
    32701 ttcggtttaa accgcagcaa ccggttggcg ttacttacta cggtaattga cgagagcgcc
    32761 attgccgctc cggcaactac cgggttaagc agtgttccag taaacggcca caaaataccg
    32821 gcggcgaccg gaataccgat gctgttgtag ataaaggcac cgagcaggtt ctgcttcatg
    32881 ttgcgcagcg ttgcgcggga aatcgcgagc gcatccgcaa cgcccatcag gctatggcgc
    32941 atcagggtaa tcgccgcggt ttcaatggca acatcactgc cgccacccat cgcaatgccg
    33001 acatccgcct gggccagcgc tggcgcgtcg ttaatgccgt cgcccaccat tgccacctga
    33061 cgtccttcac tttgcagacg tttgatcgct tcggctttac cgtccggcag cactccggca
    33121 atcacttcat caattccagc ttctttggcg atcgcattgg cggtggttgg gttatccccg
    33181 gtcaacatca ccagacgata tcccgcttta tgcaggcgtt gcagcgccgc cacgctatca
    33241 ctacgcaacg gatcgcgtac agccagcagg gctactgctt tcccgtcaat cgccagcagc
    33301 acaggcgttg ctccttgcga tgcctgagct gaaatatcgg cttcaatcgc tttagtatcg
    33361 acttgctgat catttaacaa cgcctgatta cccagcaata acgcatgacc ttctgcttca
    33421 ccgctcacgc ccagcccgcg caatgtgcgg aaaccgttga cctgcggtag ctgcatatcg
    33481 cctgctttat caaggatcgc tcgtgccaat ggatggctgg aaccttgctc cagcgccgcc
    33541 gccagacgca gcgcctgcgc ttcgtcaaca tcagcaaatg ttttcactgc gacaacctgc
    33601 ggcttccctt cggtcagcgt cccggtttta tcgaacacga cggtatcgag tgtactggcg
    33661 cgttgcagcg cgtcggcgtc ccgcaccagc acgccaaact cagccgcccg cccgacgccg
    33721 gaaataatcg acatcggcgt cgccagtccc agcgcacacg ggcaggcgat aatcagcacc
    33781 gtagtggcaa tcaccagggt atagacaatc tgcggtgccg gaccaaagaa ataccagatt
    33841 gccgcactga caagcgcaat aaccactact accggcacaa atacggctga gattttatcc
    33901 gccagctgac cgatttctgg cttgctgctc tgggcctggc gcaccatgcg aatgatcctt
    33961 gatagcgtgg tatggctgcc aaccgcactg gcacgaaaca gcacactgcc gtcctgtacc
    34021 actgtcccgg catggacgct atcgccttcg cctttttgct gcgggattgg ttcgcccgtc
    34081 agcatcgctt catccagcca tgcttcgccc tgggtaatct cgccatctac cggcacgcga
    34141 tcgccggtcg tcaggcgcag caacatacct ggctgcactt ctgccagagg cacgcttttt
    34201 tcaccttcgt cagtaaccag gcgcgccgtc ggtggggtta aatcgagtaa tttctccagt
    34261 gccttagaag aacgctgacg tgcgcgtgct tccagcatat ggccgagatt gatcagaccg
    34321 ataatcatcg cactggcttc atagtaaaga tgtcgcgctt ccatcgggaa ccactgcggc
    34381 cacaggttga cgctcatcga atagagccac gccacgcccg ttcccagcgc caccagcgta
    34441 tccatcgtcg ctgcaccgtt cagcaggctt ttccatgcac tgcggtaaaa atggccgctg
    34501 gcgaaaacca tcaccgccag ggttatcagg ccgataacca accacaggct gcggttgtcg
    34561 gcggtgacca tcatgttatc gccgatcatc ccccagacca tcaccgggat acccaccgcc
    34621 agtgcgacaa ttgcctgcca gcggaagcgc ttcatcgtag cgacggcggt ttcttgctgg
    34681 cgttcgcggc gtttcgcgtc atcttcaatc gcttccgcgc cgtagcccgc tttttccacc
    34741 gcctgcacta aatcttgtgg ggaggcactg cccatcacca gcgcagtacg ctccgccagg
    34801 tttacccgtg cctgagtgac gcccggtacg ctttgcagcg cattttgtac gcgggtgaca
    34861 cagctggcgc agctcatgcc gctcagcagc aactgctggc tgtcatcgtc atcggcggtc
    34921 gctgccggaa gcgcctcaga aaccgctgtc agtgcttccg acgggattga tgactccgcc
    34981 agcggtttag cctttgggtg gcttacagat gcgtcataac ccgcttgttt gatggtttcg
    35041 atcagctgtt ctgcactggc agtcccggta acgtgcgctt cagtgataga cacatccgct
    35101 tgttccacat ccgggcgctg ttcaagactt tctttcacgc gtttaacgca gtgaccgcag
    35161 gacaggccgt ccaggttcag gtcgatagtt tgtgacataa aacactcctt taagacagtt
    35221 ttgactggct gtgataaagg ttaaaccttc cagcaagggg aaggtcaaga aattaataaa
    35281 ccaggcgggt aaaagtccgt aaagattaaa aaatcggctc gatttgcatc aggattagac
    35341 atttatttct ttgttttcct gtagttaagt tgcgggtgct aagttaaatc aggatgcctg
    35401 aaaatcggca ctggggtgag gaattacctc ccgcatctat aaaaaggagt taacaaaaga
    35461 tgttagatgc aaacaaatta cagcaggcag tggatcaggc ttacacccaa tttcactcac
    35521 ttaacggcgg acaaaatgcc gattacattc cctttctggc gaatgtacca ggtcaactgg
    35581 cggcagtggc tatcgtgacc tgcgatggca acgtctatag tgcgggtgac agtgattacc
    35641 gctttgcact ggaatccatc tctaaagtct gtacgttagc ccttgcgtta gaagatgtcg
    35701 gcccgcaggc ggtacaggac aaaattggcg ctgacccgac cggattgccc tttaactcag
    35761 ttatcgcctt agagttgcat ggcggcaaac cgctttcgcc actggtaaat gctggcgcta
    35821 ttgccaccac cagcctgatt aacgctgaaa acgccgaaca acgctggcag cgaattttac
    35881 atatccaaca gcaactggct ggtgagcagg tagcgctctc tgacgaagtc aaccagtcgg
    35941 aacaaacaac caacttccat aatcgggcca ttgcctggct gctgtactcc gccggatatc
    36001 tctattgtga tgcaatggaa gcctgtgacg tgtacacccg tcagtgctct acgctcatca
    36061 atactgttga actggcaacg cttggcgcga cgctggcggc gggtggtgtg aatccgttga
    36121 cgcataaacg cgttcttcag gccgacaacg tgccgtacat tctggccgaa atgatgatgg
    36181 aagggctgta tggtcgctcc ggtgactggg cgtatcgcgt tggtttaccg ggcaaaagcg
    36241 gtgtaggtgg cggtattctg gcggtcgtcc ctggcgtgat gggaattgcc gcgttctcac
    36301 caccgctgga cgaagaaggc aatagtgttc gcggtcaaaa aatggtggca tcggtcgcta
    36361 agcaactcgg ctataacgtg tttaagggct gatcatgatg aacacggaag gtaataacgg
    36421 taacaaaccc ctcggtctat ggaacgtcgt ttccatcggt attggggcaa tggtgggggc
    36481 ggggatcttc gcgctgctgg ggcaggctgc gttgctaatg gaagcctcga cctggggcgc
    36541 ctttgctttt ggcggtattg tggctatgtt ttccggttat gcctatgcgc gtctgggggc
    36601 gagctatccc agcaatggcg gtattatcga cttttttcgt cgcggattag gcaacggcgt
    36661 cttttcgcta gcgctctcgt tgctatacct gttgacgctg gcggtgagca tcgccatggt
    36721 tgcccgtgct tttggcgctt atgccgtgca gtttttgcat gaaggcagcc aggaggagca
    36781 ccttattttg ctctacgcgt tggggatcat tgcggtgatg acgcttttca actccttaag
    36841 caaccatgcg gtagggcggc tggaagtgat cctcgtcggc attaaaatga tgatcctgtt
    36901 attgctgatt attgccggtg tctggtcgct gcaaccggcg catatttccg tctctgcgcc
    36961 ccccagctcc ggtgcgttct tctcctgtat tgggataacc ttccttgcct atgcgggctt
    37021 tggcatgatg gcgaacgcgg cggataaagt gaaagatccg caggtcatta tgccacgggc
    37081 gtttctggtg gcgattggcg ttaccacgtt gctttatatc tcgctggcgc tggttttgct
    37141 tagcgatgta tcggcattag agttagaaaa atatgctgat accgccgtag cgcaggctgc
    37201 ttctccgctg ctcgggcatg tgggttatgt gatcgtcgtc atcggcgctt tactggcgac
    37261 ggcttcaggc attaacgcga acctgttcgc cgtgtttaac atcatggaca acatgggcag
    37321 cgaacgcgaa ctgccgaagc taatgaataa atccctgtgg cggcagagta cctggggcaa
    37381 tatcatcgtc gtggtgttga taatgctgat gacggcggcg ctaaatttag actcactcgc
    37441 cagcgttgcc agcgccacct ttttgatctg ctatctggcg gtgtttgtgg tggcgatccg
    37501 cctgcgtcat gatattcacg cctcgttgcc gattcttatc gttggtacgt tggtgatgtt
    37561 gttggtgatc gttggcttta tctacagcct gtggtcccag ggcagccgtg cgttgatatg
    37621 gattattggt gcaatcttac tcagccttat tgtggcaatg gtcatgaagc gtaataaaac
    37681 cgtataacac ctctctgtgc gcagtacttc ctgtattatt gtggtggcgg tcgatattcg
    37741 cactggcaaa aaaacgtgct tgaatatctg ttgaaaccct ttaacaaagc acaggaggcg
    37801 ttgcgcgaac gatgaacatc agcgatgtag caaaaattac cggcctgacc agcaaagcca
    37861 ttcgcttcta tgaagagaag gggctggtga cgccgccgat gcgcagcgaa aacggttatc
    37921 gcacctacac gcagcagcat ctcaacgaac tgaccttact gcgccaggca cggcaggtgg
    37981 gctttaacct ggaagagagc ggcgagctgg tgaatctgtt taacgacccg cagcggcaca
    38041 gcgccgacgt caaacggcgc acgctggaga aggtggcgga gatcgaacga cacattgagg
    38101 agctgcaatc catgcgcgac cagctgctgg cactggcgaa tgcctgccct ggcgatgaca
    38161 gcgccgactg cccgattatc gaaaatctct ccggctgctg tcatcatcgg gcagggggat
    38221 taagacgaga cggctcggat atgtagggtt atcccttcta tcgcaatgac ttcaacatgc
    38281 gtacctgcac cgagatcctc gctggcgctg acaggccatg aactgtcacc gacgcgcata
    38341 tgaccgcgcc cgttgaccag cggagattcc agcacaaaac gtcggccaat cagctgctgc
    38401 ccgcgctggt ttaaatgact atcgctgtgc ttttgttcgc gcacccgccg cgacaaccat
    38461 ttccaccaca gccaggcggc gagcagcgtc aggatggcaa acattacccc ttgccactcc
    38521 caacccagcg gcaccagcca gaccaccagg ccggtaatca ccgctgccac gccgctccac
    38581 aacaaataac catttccgcc cagcatctcg gctgccagca gcaacccgcc gagactgagc
    38641 cagaaaatat gtggatgaac gaccattaac tccatcatgg ctgagtccgt ttgttggcgc
    38701 tgtctttcac cagctcggca atcccggcaa tcgaccccat caggctgctg gcctctaatg
    38761 gcatcatcac tactttgctg ttactggagg aaccgatctg ctgtaacgct tcggtgtatt
    38821 tttgcgctac gaagtagttc accgcctgaa tatcaccgga ggcgatagct tcagacacca
    38881 ttttggtggc gcgggcttct gcttcggcgg aacgttcacg cgcttcagcc tgtaaaaacg
    38941 ccgactgacg ttcgccttcc gctttcagga tttgcgactg tttttcacct tcggctttga
    39001 ggatttccgc ctgacggatc ccttccgctt caagaatgta agcgcgtttg gtacgttccg
    39061 ctttcatctg cgcgttcatt gaagagataa gctctgccgg tgggcgcacg tcgcgaattt
    39121 caatacgggt gactttaatc ccccacgggt tggtggcttc atcgacaata cgcagcaggc
    39181 gtgagttgat gctgtcgcgc tgagagagca tttcgtcaag ttccattgaa cccaacacgg
    39241 tacggatgtt agtcatggtc aggttgatga tcgccagctc cagattgctg acttcatagg
    39301 cagcgcgtgg cgcgtcaatc acctgaataa aacagactgc gtcgatggta acgttggcgt
    39361 tatctttcga gataacttcc tgggaaggga tatcgagcac ttgctccatc atattgatct
    39421 tgcgaccaat gcgatccata aacggcacca ccagactgag ccccggctgt aacgttttgg
    39481 tatagcgacc aaaacgttct accgtccatt gatagccctg cggcacgatt ttgacgcccg
    39541 cgccgacaat gaccagcgcg acaaaaatga gaatcgggat aaagataagc atcggaaaaa
    39601 cctcctgttg taccgtccat aatcagcaaa attgctgctt gattaaacaa attatacctg
    39661 attactgaaa gagagttccc ccttattcct gcgaaggata aactgttttt agtaaaaatc
    39721 agaaaaaggg aacagcgatg caggaaaata gtcctttgct tcagctacaa aacgtaggat
    39781 atctggcggg tgatacgaag attcttaata acatcaattt ttcgctgcgt gctggcgaat
    39841 ttaagttaat taccggtcct tctggttgtg gcaaaagtac gctgctaaaa atagttgctt
    39901 cattgatcag cccaaccagc ggaacgttac tgtttgaagg tgaggatgtc agcacactaa
    39961 aaccagaaat ctaccgccag caagtctctt actgcgccca gacgccgacg ctgtttggcg
    40021 atacggtata cgataatctg atctttccct ggcagatccg tgaccagcag cctgacccgg
    40081 ccatttttct cgattttctc gaacgcttcg ctttgccgga cagcattttg acgaagaata
    40141 tcgccgagct atctggtggt gaaaaacaac gcatctcatt gattcgtaac ctgcaattta
    40201 tgccgaaggt tttattgctg gatgaaataa ccagtgcgct ggatgaaagt aataaacata
    40261 acgtcaatga gatgatccat cgttatgtgc gcgagcaaaa tattgccgtg ctgtgggtga
    40321 cacacgataa agacgaaatt aatcatgcgg ataaagtgat tacactgcaa ccgcatgccg
    40381 gagaaatgca ggaagcacgc tatgaactcg cataatatta ctaacgaatc attagcactg
    40441 gcattaatgc tggtggtggt ggcaatctta attagccata aagaaaaact ggcgctggag
    40501 aaagatattc tctggagcgt cgggcgagcg ataattcagc tgattattgt cggctatgtg
    40561 ctgaagtata ttttcagcgt ggatgatgcc agcctgacat tattgatggt gttatttatc
    40621 tgctttaatg cggcgtggaa cgcgcaaaaa cgcagtaaat atattgctaa agcttttatc
    40681 tcatcgttta ttgccattac ggtcggggcg ggaattaccc tggcggtgct gattctctca
    40741 ggggcgattg aatttatccc gatgcaggtg atccctgtcg ccgggatgat tgccggtaac
    40801 gccatggtag cggtggggtt gtgttacaac aatttagggc aacgggtcat tagcgaacag
    40861 caacagattc aggagaaact gagtcttggc gcgacgccga agcaggcttc agcgatattg
    40921 attcgcgaca gtattcgcgc ggctttaatt ccgacggtcg attcagcaaa aacggttggc
    40981 ttagtgagtt taccaggaat gatgtccggg ctgatatttg ccgggattga tccggtgaag
    41041 gcgattaaat atcagattat ggtgaccttt atgctgctct caaccgccag cttgtcgacc
    41101 attattgcct gctatttaac ctatcgtaag ttttataatt cgcgccacca gttggtggtg
    41161 actcaattga agaagaaatg atgcgatgcc ggatgcgcag catcgcatcc ggcgttgtgg
    41221 ttcatgtgcc gaacaacgga atatcaatac aacaatgcat acagctggcg gcgatacttc
    41281 gacgccagtg catcacccgt acccagcgca gcgaggatct cctggaacgt tttacgcgtc
    41341 tgaccgtctg cggcggtgag atctttacgc agatgcccga acagcaactc cagcgcctct
    41401 tcattgcgcc caacctgatg cagttgcagc gccagttgcg tcgccagtgc ggcatcttct
    41461 ggattctccg ccacctgctg ttgcaactgt tgaatttccg gcttatcagc cgcctgcttc
    41521 agcagttcga tttgcgctac cagcccctgg tagcgggtgt cctgatcctg caacggaatg
    41581 gttttcagca ccgcttccgc atcttcagaa cggttcagcg caatcagcgt ttctgccagc
    41641 agcaggccga tctccccgtt ctgattcgac aactgccagg cgtcttgcag caatggcagg
    41701 gcatcggtgt aattgccttc ctgcatcagt tgcatcgcct gctgcgcttt cagctcttct
    41761 tcgcgcggca gcactttatc cagcagggcg cggatcgcct cttccggttg cggcccctgg
    41821 aagccatcta ccggttgccc gttctggaac agatacacgg tcggaatcgc gcgcagacca
    41881 aactgggcgg caatcatctg ctccgcgtcg cagtccagct tcgccagaat aaattgcccg
    41941 ttgtactgcg ccgcgaggct ttccaggatc ggggttaact gcaaacagtg ctggctacgt
    42001 tcagaccaaa aatagaacag caccggagtg gtcatcgact gttcaagaac ctgttgcagg
    42061 ttagattcgt taatgttgac aatattttct acggacatgg agtcgctctc tgttgtcgat
    42121 attttctttg acatgggggc ttaagcgcgc gcttcaactc acccctgcaa tattttgtcc
    42181 atcacgcgcc caggcagcag gcgctcgagc accattactg cccaggtcac cagcgttacc
    42241 ggatagcgca tcttcggctt ctcgctaata aaagcatggc gcactttgtc gaccaccgct
    42301 tccggtccca acgtaaagcg ggcggcgatg ccgggatttt cgactggttt atcactttgc
    42361 gtctggttga cgttgtcggt gaagcgagta cgaatgggac cgggttcgat caggctgact
    42421 ttaattccgc tgtggcgtag ctccatgcgc agcgcgtctg accacgcctc cagcgcatat
    42481 ttactggccg cgtaagcgcc acgacccggc gtggagatta atcccatcac cgatgatgtc
    42541 atcacaatac gcccttcacc gtgcggtaac atcgcgggta acaggcgcat ggtgagctgg
    42601 tgtgcgccga aaaagttggc ggaaaactgc tgttccatct gcgcacggct gatggtggaa
    42661 agggggccat acatgccgaa tccggcattg ttaaagatcc catacagaca attatcggtc
    42721 agggcgatca cctcgtctgc tgcgcgatta acactttctg gtgaatccag atcgatcaac
    42781 acgccggtaa atcccatgtt gttcatacgc tcaacatcat tcggttttcg gcaacctgcc
    42841 agcacatgaa aaccctggcg ttttaattcg agcgcgcttt ccaggccaat tccactggaa
    42901 catccggtaa ttaagaccga tttttgcata actttacctg tcaggatctc cgttgcttta
    42961 tgagtcatga tttactaaag gctgcaactg cttcgccatc cagtcggcaa taaacggctg
    43021 ggcgtcgcgg ttgggatgaa taccgtcatc ctgcatccat tgtggcttga ggtagacctc
    43081 ttccataaaa aagggcagca gcggaacatc aaactctttg gcgagtttgg ggtaaatggc
    43141 gctaaaggct tcattataac ggcgaccata gtttgcaggc agacgtattt gcattaacaa
    43201 tggttcagcg ttggcggctt tgacatcctg caaaatctgg cgcagcgttt gctcggtttg
    43261 ctgtggctga aaaccacgca aaccatcatt gcgcccagtt caaccagcac ccaacgcggc
    43321 tgatgctgtt tgagcagagc cggaaggcgc gccagccctt gttgcgaggt gtcgccgctg
    43381 atgctggcat taactaccga cgttttactc tgccacttat cattcaacaa ggcaggccag
    43441 gccgcgctgg cagacattcg atacccggcg ctcaggctat cacccagaat caataacgtg
    43501 tccgctgcgg cggcacggaa ggttaacagg accaggaaca ggaagggcaa atgccagcgg
    43561 aaaacattgt tgaagttcat catcttaaga agtccgtcgg tcagggggag catgaactct
    43621 ccatcctcac cggagttgag ctggttgtca aacgtggcga gaccatcgca ctggtgggcg
    43681 agtcgggatc gggtaagtca accttgctgg cgatcctcgc cgggcttgat gacggcagca
    43741 gtggcgaagt gagtctggtg ggacaaccgc tacataatat ggacgaagaa gcgcgggcaa
    43801 agttgcgcgc gaagcacgtc ggctttgttt ttcagtcatt tatgttaatt cctaccctta
    43861 acgcgctgga aaacgtcgag cttccggctc tgctgcgcgg tgagagtagc gcggaaagtc
    43921 gtaacggggc gaaagcgttg ctcgaacagt tagggctggg taaacgtctg gatcatcttc
    43981 cggcacagct ttccggcggt gaacagcaac gagtggcgct ggcacgagcc tttaatggtc
    44041 gacctgatgt gctgtttgcc gacgaaccca ccggcaacct tgaccgccag acgggcgata
    44101 aaattgccga cctgctgttt tccctcaacc gtgaacatgg caccacgttg attatggtga
    44161 cccacgacct gcaactggcg gcacgctgcg accgctgctt acggctggtg aacgggcagt
    44221 tgcaggagga agcatgattg cacgttggtt ctggcgcgaa tggagttcgc cgtcgctatt
    44281 aattgtctgg ctggcgctaa gcctggcggt ggcctgcgtg ctggcgctgg gcaatatcag
    44341 cgatcgcatg gagaagggct taagccagca aagccgtgag tttatggcgg gcgatcgggc
    44401 gttgcgcagt tcacgcgaag tgccgcaagc gtggctggag gaagcgcaaa agcgcggcct
    44461 gaaagtcggc aagcagctga ctttcgccac aatgaccttt gcaggcgaca caccgcagct
    44521 ggcgaacgtc aaagcggtgg atgatatcta cccgatgtat ggcgatctgc aaactaatcc
    44581 ccctggcctg aaaccgcagg cgggcagcgt attgctggcc ccacgcctga tggcactgct
    44641 taacctgaaa acgggcgaca ccattgacgt gggcgatgcc accttgcgga ttgccggaga
    44701 agtgattcag gaaccggatt ccggttttaa ccccttccag atggctccgc gtctgatgat
    44761 gaatctggcg gatgtcgata aaaccggagc cgtgcagccg gggagtcggg tcacctggcg
    44821 ttataaattc ggcggcaacg agaaccagct cgacggctat gagaaatggt tgttacctca
    44881 gcttaaaccc gaacaacgct ggtacggtct ggaacaggac gaaggcgcgc tggggcgatc
    44941 gatggaacgc tcgcaacagt tcctgctgct ttcggcgctt ctgaccttgc tgctggcagt
    45001 ggcagcggtg gcggtagcga tgaatcatta ctgtcgcagt cgctacgatc tggtggcgat
    45061 cctcaaaacg ctgggggcag ggcgagcgca actgcgtaag ctaatcgtcg gtcagtggtt
    45121 gatggtgctg acgctttcag ccgttaccgg tggggccata ggcctgttgt tcgaaaacgt
    45181 gttgatggtg ctgctcaagc ccgttctgcc tgctgcacta ccgccagcca gcctctggcc
    45241 gtggctgtgg gcgcttggca ccatgacggt catctcgctg ctggtggggc tacgaccata
    45301 ccgcttgttg ctggcaacgc agcctttacg cgtattacgt aatgatgtgg tcgcgaacgt
    45361 ctggccgctg aagttttatc tgccgattgt cagtgtggtg gttgtgctgc tgctcgccgg
    45421 attaatgggt ggcagcatgc tgctttgggc ggtgctggcg ggcgcggtag tactggcttt
    45481 gctgtgcggt gtgctgggct ggatgctgct gaatgtactt cgccgcatga cgctgaaatc
    45541 gctgcctctg cgcctggcgg ttagccgcct gttacgtcag ccgtggtcaa cgttaagcca
    45601 gctttcggca ttttcgctct cctttatgct gctggcactg ctgctggtgc tgcgtggcga
    45661 tctgctcgac cgctggcagc agcagctacc gccagaaagc ccgaactact ttttaattaa
    45721 catcgccaca gaacaggtag caccgctaaa agcgttcctc gcggaacatc atatagtccc
    45781 ggaatcattt tatccggtag tgcgggcgcg gctgacggcg attaatgata agccgacaga
    45841 aggtaatgaa gatgaggcgc ttaatcgcga actcaatctt acctggcaaa atacgcggcc
    45901 cgatcataat ccgattgtcg ccggtaactg gccgccaaaa gccgatgaag tgtcgatgga
    45961 agaggggctg gcgaaacgct taaacgttgc cctcggtgat accgtgactt ttatgggcga
    46021 tacccaggag ttccgcgcta aagtgaccag cctgcgcaaa gtggactggg aaagtctgcg
    46081 gcctaatttc tattttattt tccctgaagg ggcattagac gggcaaccgc agagctggct
    46141 taccagtttc cgctgggaga atggcaacgg catgttgacg caactcaacc gccagttccc
    46201 gaccattagc ctgttagaca ttggcgcgat tttaaaacag gtcggtcagg tgctggagca
    46261 ggtaagtcgg gcgctggagg tgatggtggt gctggtcacc gcctgcggta tgttgctgtt
    46321 gctggcacag gtgcaggtgg gaatgcgcca gcgtcatcag gagctggtgg tatggcgcac
    46381 actcggtgcg gggaaaaaac tgctgcgcac gacattgtgg tgtgatgttc gccatgcttg
    46441 ggtttgtttc cggcctggtg gccgcaattg gtgcggaaac ggcactggca gtgttgcagg
    46501 cgaaagtgtt tgatttcccg tgggagccag actggcgatt gtggattgtt ctgccgtgca
    46561 gcggtgcgct gctgctgtcg cttttcggcg gctggctggg tgcgcgactg gttaagggta
    46621 aggcgctgtt caggcagttt gtggggtgat gacaggtgtt agattgcctg atgcgctacg
    46681 cttatcaggc ctacgaagag cgtgcaatat attgattttg caagattgtg taggccggat
    46741 aaggcgttta cgccgcatct ggcatgaaca gtgtgcattt tggcaataaa tatgaaacca
    46801 actgtcatgt cggttttttt aaaggccgaa ggaggtaaac cttcggcctt agctacgttt
    46861 cctcagttat tcaataaact gacggatcag ttccagcagc gcgattagcg ccttaagaac
    46921 gatgagaact ttatcgagaa aagttttcat ccgttgctcc cttaagggag aaagacaatc
    46981 acagcttacc cttacgttgc ctataactat gattttcttc atgtgctaga tgaggactgt
    47041 ctctctccaa attagagcac cagaatcagc accacctgta tcctgaccag gacttaaaag
    47101 tacttttccg cactgcggta atcccttgag gcagtatcac tactagcgtc gggattgtat
    47161 cgccaacata gctgtttgaa tatgtaggca tcctgactta taattcactt gctagatgag
    47221 gactgtttaa tttctgcccg gaaccaccaa gtcttgggcg gaaaaaataa aaaaaccgac
    47281 tgtcatgtcg gttttttata tctgtattct ggtgcaacca ttttgttctc acgacaatcg
    47341 ccgtttcaga ataccgccca tcactgcgcc ttaacccata ccgccacttc cgcccactca
    47401 ccgcgaaaga caactttttc cgcttttttc tcaagctgat agcgatacat tgggtcgtaa
    47461 tattctttaa gtaacggcac cagccaggcc agatgaccgt cggtgctgcc ggtggtgagt
    47521 tgcgttgtca gcgccgtatc cagctgggca gcgagttcgt tatagcgctg taaccccagc
    47581 cgacgcttaa tcgccgaaag tccgtgatgc aggtattcgc aatattcctg ccagccctgt
    47641 tcgtcgccat aagcgtgggt aaaatcatga tgcatacgca agaaatactc ttcattcagg
    47701 cgttcaagac ggatctcaaa cggatcttct accaccgcaa tcgccgcctg agtcattcgc
    47761 tcgcgcagac attccggcag gtgattcgaa ccgatcatcc ggctttcatc ttccagcacc
    47821 cacaggcgca aatcctgacg ggcgtcggtt tttagcattt cggcagccag caggttttca
    47881 aaactcgcct ggctaagttg tggttgtaac gtgcgaccaa acgccgaacc gcgatgacgc
    47941 gccagccctt ccagatcaac accgtttggc tgttgttgca ctaatagcgt tttaccgctg
    48001 ccggtacaac cgccaatcag cactatcggt ttttgtgcca gttcaatggt cgcctgaatc
    48061 gcggtctggc gcagtgcctt ataaccgcct tccaccagcg gataatcaat ccccgcttca
    48121 tgcagccagc gttgcacaat atgtgaacgt tgaccgccac gggcgcagca gagaatacct
    48181 tgcggatttt gcaggcacgc tgcccgccag gcgtacatac gctgctgacg aatttcaccc
    48241 gccaccagtt tatgccccag cgccagcgct gcgtctgagc cttgctgttt atagcaggtg
    48301 ccaacggagg cgcgttcatc gttatccatt aacggcagat tgatagcggc gggcattgcg
    48361 ccttgctcaa actcgatagg ggcgcgaaca tcaataatgg gcgtatcagc aatcagcagg
    48421 gcacgatagt cctgttccgt gtgtctctct tgcatagtta aaagtgaacc tcaaatcagc
    48481 ttgcgcgcta ttttacgcgc caacgcgcaa ggaaacttga tttttaactg cgtggggtgc
    48541 cgaaaatttc taaaaatccg ctgatttccg gcctgtgttg ggtaaacaag gtcacaatat
    48601 cttctaccgc tttgcagctg ccaaatttgc gccatgccag actcaatggc gaaggagggc
    48661 gcatcgttgg ggttacccgg ctgaccagtt gttgattatc gatcattgac tggcaaagtg
    48721 attttggcaa aaaaccaatg ccaacacccg ccagatgggc ggcgattttc gtttccatat
    48781 cgggaacaat aatctctttt tgccccggca atcgccaggc gacgcgtttg gttaaggtgc
    48841 cggcgctgtc ttcaatattg accgccggaa agcgacgcaa ctgcgcttct gttagcggct
    48901 cttcaacgtt cgccagcgga tgatccgccg ccatgacaaa tcgccattgc accgatccta
    48961 agggatcaag actaaaggta tttgccagcg cctcagttcc cgtgaccccg atagccagcg
    49021 aaaaaccttc gtacaatagc gagtcccaga cgcccatata gatttgtcgg gagatgtgaa
    49081 actgggtaaa ggggtaacgc tcattcagcc acgccagcaa ctgggcgacg gcctgggggt
    49141 tatagagcag gttgttgatg acaatattca cctggcgttc cacgccatca ttcacctgtt
    49201 gcagctcgct gggcatactt tccagccagc tcagccagtc tctggcctgg gaaagtagat
    49261 gctcgccagc cgctgtcaac gttacgctac gagtcgtacg gaaaaacagc gctactccgg
    49321 tattctcttc cagaagttta atgcgataac tgatcgtcgc cgtggtttta cataatcgtt
    49381 ctgccgcttt tgaaaaactt cctgtttcag caaccgcaat gaaagtccgc aaggtttctg
    49441 gatcgaacat cttcaggtat ccccttttaa atccgcaagt tgcgtgattt tcttatcctc
    49501 tgatttatca gtatttttac atgataatcc tgttcaattt gtggactaaa tctagttttg
    49561 gaaaaacatt ccaacttttg tattgatgtt gttctcttaa ggttttagat tgcctgttat
    49621 tgaaaccaag ctgaccggtc ggcggtggtt gaacggaatt atgttacaag gacaaaaaga
    49681 tgaaacttca ggtattaccg ttaagtcagg aagcctttag tgcttatggc gacgtaatcg
    49741 aaacgcagca acgggatttt ttccatatta acaatggcct ggtggagcgt taccacgatt
    49801 tggcgctggt tgagattctt gagcaagacc gtacgcttat cagcattaac cgcgcgcaac
    49861 cggcgaatct gccgctgacc attcacgaac tcgaacgtca tccgctgggc actcaggcct
    49921 ttatcccaat gaaaggtgaa gtgtttgtgg tggtcgtggc gttaggcgac gacaaaccag
    49981 acctgtcaac gctgcgggcg tttatcacca acggtgaaca gggggtgaat taccatcgta
    50041 acgtctggca tcacccactt ttcgcctggc agcgcgtcac cgattttctg accatcgatc
    50101 gcggcggcag tgacaactgt gatgttgaaa gtattcctga acaggaactc tgttttgcgt
    50161 gacgcctgca accgacttgc ataagataaa ctaattgttc attgtttatg ctcacttgta
    50221 ggtcggagtt aacgtaggta tgacggaagt tagacggcgc ggcaggccag gacaggcgga
    50281 gcctgtggca cagaagggcg cacaggcgtt agagcgggga attgcgattc tgcaatatct
    50341 ggaaaaaagt gggggaagtt cgtcggttag cgatatttct ctcaatctgg atttgccgct
    50401 ctccacgacc tttcgcttgc tgaaggtttt acaagcagcg gattttgtct atcaggacag
    50461 tcaattaggc tggtggcata taggattagg tgtctttaac gtcggtgcgg cgtacatcca
    50521 taaccgcgat gtcctctccg tcgccgggcc gtttatgcgc cgcctgatgt tactttccgg
    50581 cgaaacggtc aatgtcgcga tccgtaacgg caatgaagcg gtattaattg gtcagttaga
    50641 gtgtaaatcg atggtcagga tgtgtgcgcc actgggcagt cgtctgccgc tgcatgcttc
    50701 cggtgcgggc aaagcgctgc tttatccgct ggcggaagag gagttgatga gcatcattct
    50761 gcaaaccggt ttgcagcagt ttacgccaac tacgcttgtg gatatgccca ccttgctgaa
    50821 ggacctggaa caagcgcgtg aactgggcta taccgtagat aaagaagagc atgttgtagg
    50881 tctgaattgc atagcttcag caatttacga tgatgtcggt agagttgttg ccgctatctc
    50941 catctccggg ccttcatcaa gactgacaga agatcgtttt gtcagtcagg gtgagctggt
    51001 cagagacacc gcccgcgata tcagcacggc gttgggactg aaagcacatc catagtgtct
    51061 gtcgcatccc gctctgcgga gcgggttttt ttgacaaaat ttgaaagttg gaaaattttt
    51121 ccaattaata gaggtaggaa taaaatggca aaaatgagag ccgttgacgc ggcaatgtat
    51181 gtgctggaga aagaaggtat caccaccgcc ttcggcgttc cgggagctgc aattaatccg
    51241 ttctactcag cgatgcgtaa gcacggcggt attcgtcaca ttctggcgcg tcatgtggaa
    51301 ggtgtttcgc acatggcgga aggttatacc cgcgcaacgg cagggaatat cggcgtatgt
    51361 ctggggactt ccggtcctgc gggcacggac atgatcaccg cgctctattc cgcttctgct
    51421 gattccattc ctattctgtg cattaccggc caggcaccgc gcgcccgtct gcataaagaa
    51481 gattttcagg ccgtagatat tgaagcaatt gctaaaccgg tcagcaaaat ggcggttaca
    51541 gttcgtgaag cggcgctggt gcctcgcgtg ctgcaacagg catttcacct gatgcgttct
    51601 ggtcgtccgg gtccggtact ggtggattta ccgttcgacg ttcaggttgc ggaaatcgag
    51661 tttgatcctg acatgtacga accgctgccg gtctacaaac ctgctgccag ccgtatgcag
    51721 atcgaaaaag ctgtagaaat gttaatccag gccgaacgtc cggtgattgt tgccgggggc
    51781 ggggtaatca atgctgacgc agctgcactg ttacaacagt ttgctgaact gaccagcgtt
    51841 ccggtgatcc caacgctgat gggctggggc tgtatcccgg acgatcatga actgatggcc
    51901 gggatggtcg gtctgcaaac cgcgcatcgt tacggtaacg caacgttgct ggcgtccgac
    51961 atggtgtttg gtatcggtaa ccgttttgct aaccgtcata ccggttcggt agagaaatac
    52021 accgaagggc gcaaaatcgt tcatattgat attgagccga cgcaaattgg tcgcgtgctg
    52081 tgtccggatc tcggcattgt ctctgatgct aaagcggcgc tgacactgct ggttgaagtg
    52141 gcgcaggaga tgcaaaaagc gggtcgtctg ccgtgtcgta aagaatgggt cgccgactgc
    52201 cagcagcgta aacgcacttt gctgcgcaaa acccacttcg acaacgtgcc ggtgaaaccg
    52261 cagcgcgtgt atgaagagat gaacaaagcc tttggtcgcg atgtttgtta tgtcaccact
    52321 attggtctgt cacaaatcgc tgcggcacaa atgctgcatg tctttaaaga ccgccactgg
    52381 atcaactgtg gtcaggctgg tccgttaggc tggacgattc cggcggcgct gggggtttgt
    52441 gccgctgatc cgaaacgcaa tgtggtggcg atttctggcg actttgactt ccagttcctg
    52501 attgaagagt tagccgttgg cgcgcagttc aacattccgt acatccatgt gctggtgaat
    52561 aacgcctatc tcggcctgat tcgtcagtca caacgcgctt ttgacatgga ctactgcgtg
    52621 caactcgctt tcgagaatat caactccagt gaagtgaatg gctacggtgt tgaccacgta
    52681 aaagtagcgg aaggtttagg ttgtaaagct attcgggtct tcaaaccgga agatattgcg
    52741 ccagcctttg aacaggcgaa agccttaatg gcgcaatatc gggtaccggt agtcgtggaa
    52801 gttattctcg agcgtgtgac caatatttcg atgggcagcg aactggataa cgtcatggaa
    52861 tttgaagata tcgccgataa cgcagcggat gcaccgactg aaacctgctt catgcactat
    52921 gaataaggga gataaatgat gttacgtttc tctgctaatt tatcgatgtt atttggtgaa
    52981 tatgattttc tcgcccgttt tgagaaagct gcgcagtgtg gttttcgcgg cgttgaattt
    53041 atgtttcctt atgactacga cattgaagaa ttaaaacagg tactggcgag taataaactc
    53101 gaacatacgc tgcacaattt accggcaggt gactgggcgg cgggcgagcg cggtattgcc
    53161 tgtattcctg gccgtgaaga agagtttcgg gatggcgtag cagcagcgat tcgt
//
LOCUS       DAEEUE010000017        53198 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.017, whole genome shotgun sequence.
ACCESSION   DAEEUE010000017 DAEEUE010000000
VERSION     DAEEUE010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53198
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.017"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            98..397
                     /locus_tag="KLK84_001071"
     CDS             98..397
                     /locus_tag="KLK84_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE2008491.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            407..931
                     /gene="ygaP"
                     /locus_tag="KLK84_001072"
     CDS             407..931
                     /gene="ygaP"
                     /locus_tag="KLK84_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HBE2008492.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(978..1382)
                     /gene="stpA"
                     /locus_tag="KLK84_001073"
     CDS             complement(978..1382)
                     /gene="stpA"
                     /locus_tag="KLK84_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HBE2008493.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            2050..2499
                     /gene="alaE"
                     /locus_tag="KLK84_001074"
     CDS             2050..2499
                     /gene="alaE"
                     /locus_tag="KLK84_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HBE2008494.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2536..2880)
                     /locus_tag="KLK84_001075"
     CDS             complement(2536..2880)
                     /locus_tag="KLK84_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HBE2008495.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            3032..3262
                     /locus_tag="KLK84_001076"
     CDS             3032..3262
                     /locus_tag="KLK84_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2008496.1"
                     /translation="MYNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3609..3854
                     /gene="nrdH"
                     /locus_tag="KLK84_001077"
     CDS             3609..3854
                     /gene="nrdH"
                     /locus_tag="KLK84_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBE2008497.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3851..4261
                     /gene="nrdI"
                     /locus_tag="KLK84_001078"
     CDS             3851..4261
                     /gene="nrdI"
                     /locus_tag="KLK84_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBE2008498.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4234..6378
                     /gene="nrdE"
                     /locus_tag="KLK84_001079"
     CDS             4234..6378
                     /gene="nrdE"
                     /locus_tag="KLK84_001079"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBE2008499.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRASPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6388..7347
                     /gene="nrdF"
                     /locus_tag="KLK84_001080"
     CDS             6388..7347
                     /gene="nrdF"
                     /locus_tag="KLK84_001080"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBE2008500.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7702..8904
                     /gene="proV"
                     /locus_tag="KLK84_001081"
     CDS             7702..8904
                     /gene="proV"
                     /locus_tag="KLK84_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HBE2008501.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8897..9961
                     /gene="proW"
                     /locus_tag="KLK84_001082"
     CDS             8897..9961
                     /gene="proW"
                     /locus_tag="KLK84_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HBE2008502.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10019..11011
                     /gene="proX"
                     /locus_tag="KLK84_001083"
     CDS             10019..11011
                     /gene="proX"
                     /locus_tag="KLK84_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HBE2008503.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11203..12382
                     /locus_tag="KLK84_001084"
                     /pseudo
     CDS             11203..12382
                     /locus_tag="KLK84_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12506..13243
                     /gene="ygaZ"
                     /locus_tag="KLK84_001085"
     CDS             12506..13243
                     /gene="ygaZ"
                     /locus_tag="KLK84_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HBE2008504.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13233..13568
                     /gene="ygaH"
                     /locus_tag="KLK84_001086"
     CDS             13233..13568
                     /gene="ygaH"
                     /locus_tag="KLK84_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HBE2008505.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13659..14189
                     /gene="emrR"
                     /locus_tag="KLK84_001087"
     CDS             13659..14189
                     /gene="emrR"
                     /locus_tag="KLK84_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HBE2008506.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14316..15488
                     /gene="emrA"
                     /locus_tag="KLK84_001088"
                     /pseudo
     CDS             14316..15488
                     /gene="emrA"
                     /locus_tag="KLK84_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15505..17043
                     /gene="emrB"
                     /locus_tag="KLK84_001089"
     CDS             15505..17043
                     /gene="emrB"
                     /locus_tag="KLK84_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HBE2008507.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFDAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17107..17622)
                     /gene="luxS"
                     /locus_tag="KLK84_001090"
     CDS             complement(17107..17622)
                     /gene="luxS"
                     /locus_tag="KLK84_001090"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBE2008508.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17772..19328)
                     /gene="gshA"
                     /locus_tag="KLK84_001091"
     CDS             complement(17772..19328)
                     /gene="gshA"
                     /locus_tag="KLK84_001091"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HBE2008509.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19401..19829)
                     /locus_tag="KLK84_001092"
     CDS             complement(19401..19829)
                     /locus_tag="KLK84_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBE2008510.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19826..20392)
                     /gene="yqaB"
                     /locus_tag="KLK84_001093"
     CDS             complement(19826..20392)
                     /gene="yqaB"
                     /locus_tag="KLK84_001093"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HBE2008511.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(20540..20616)
                     /locus_tag="KLK84_001094"
     tRNA            complement(20540..20616)
                     /locus_tag="KLK84_001094"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20580..20582),aa:Arg,seq:acg)
     gene            complement(20681..20757)
                     /locus_tag="KLK84_001095"
     tRNA            complement(20681..20757)
                     /locus_tag="KLK84_001095"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20721..20723),aa:Arg,seq:acg)
     gene            complement(20822..20898)
                     /locus_tag="KLK84_001096"
     tRNA            complement(20822..20898)
                     /locus_tag="KLK84_001096"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20862..20864),aa:Arg,seq:acg)
     gene            complement(20963..21039)
                     /locus_tag="KLK84_001097"
     tRNA            complement(20963..21039)
                     /locus_tag="KLK84_001097"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21003..21005),aa:Arg,seq:acg)
     gene            complement(21043..21135)
                     /locus_tag="KLK84_001098"
     tRNA            complement(21043..21135)
                     /locus_tag="KLK84_001098"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21099..21101),aa:Ser,seq:gct)
     gene            complement(21451..21636)
                     /gene="csrA"
                     /locus_tag="KLK84_001099"
     CDS             complement(21451..21636)
                     /gene="csrA"
                     /locus_tag="KLK84_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBE2008512.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(21871..24501)
                     /gene="alaS"
                     /locus_tag="KLK84_001100"
     CDS             complement(21871..24501)
                     /gene="alaS"
                     /locus_tag="KLK84_001100"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBE2008513.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(24629..25129)
                     /gene="recX"
                     /locus_tag="KLK84_001101"
     CDS             complement(24629..25129)
                     /gene="recX"
                     /locus_tag="KLK84_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBE2008514.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(25372..26433)
                     /gene="recA"
                     /locus_tag="KLK84_001102"
     CDS             complement(25372..26433)
                     /gene="recA"
                     /locus_tag="KLK84_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBE2008515.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(26513..27010)
                     /gene="pncC"
                     /locus_tag="KLK84_001103"
     CDS             complement(26513..27010)
                     /gene="pncC"
                     /locus_tag="KLK84_001103"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HBE2008516.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(27155..28240)
                     /gene="mltB"
                     /locus_tag="KLK84_001104"
     CDS             complement(27155..28240)
                     /gene="mltB"
                     /locus_tag="KLK84_001104"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HBE2008517.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            28496..29058
                     /gene="srlA"
                     /locus_tag="KLK84_001105"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             28496..29058
                     /gene="srlA"
                     /locus_tag="KLK84_001105"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            29055..30014
                     /gene="srlE"
                     /locus_tag="KLK84_001106"
                     /gene_synonym="gutE"
     CDS             29055..30014
                     /gene="srlE"
                     /locus_tag="KLK84_001106"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBE2008518.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSSFIYQ"
     gene            30025..30396
                     /gene="srlB"
                     /locus_tag="KLK84_001107"
                     /gene_synonym="gutB"
     CDS             30025..30396
                     /gene="srlB"
                     /locus_tag="KLK84_001107"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBE2008519.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            30400..31179
                     /gene="srlD"
                     /locus_tag="KLK84_001108"
     CDS             30400..31179
                     /gene="srlD"
                     /locus_tag="KLK84_001108"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBE2008520.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            31284..31643
                     /gene="gutM"
                     /locus_tag="KLK84_001109"
                     /pseudo
     CDS             31284..31643
                     /gene="gutM"
                     /locus_tag="KLK84_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
     gene            31710..32483
                     /gene="srlR"
                     /locus_tag="KLK84_001110"
                     /gene_synonym="gutR"
     CDS             31710..32483
                     /gene="srlR"
                     /locus_tag="KLK84_001110"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HBE2008521.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            32476..33441
                     /gene="gutQ"
                     /locus_tag="KLK84_001111"
     CDS             32476..33441
                     /gene="gutQ"
                     /locus_tag="KLK84_001111"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HBE2008522.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(33438..34952)
                     /gene="norR"
                     /locus_tag="KLK84_001112"
     CDS             complement(33438..34952)
                     /gene="norR"
                     /locus_tag="KLK84_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HBE2008523.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVL
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            35139..36578
                     /gene="norV"
                     /locus_tag="KLK84_001113"
     CDS             35139..36578
                     /gene="norV"
                     /locus_tag="KLK84_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HBE2008524.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            36575..37708
                     /gene="norW"
                     /locus_tag="KLK84_001114"
     CDS             36575..37708
                     /gene="norW"
                     /locus_tag="KLK84_001114"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HBE2008525.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(37836..40088)
                     /gene="hypF"
                     /locus_tag="KLK84_001115"
     CDS             complement(37836..40088)
                     /gene="hypF"
                     /locus_tag="KLK84_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HBE2008526.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(40241..40768)
                     /gene="hydN"
                     /locus_tag="KLK84_001116"
     CDS             complement(40241..40768)
                     /gene="hydN"
                     /locus_tag="KLK84_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HBE2008527.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(40917..41927)
                     /gene="ascG"
                     /locus_tag="KLK84_001117"
     CDS             complement(40917..41927)
                     /gene="ascG"
                     /locus_tag="KLK84_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HBE2008528.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(41941..42060)
                     /locus_tag="KLK84_001118"
     CDS             complement(41941..42060)
                     /locus_tag="KLK84_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HBE2008529.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(42070..43779)
                     /gene="hycE"
                     /locus_tag="KLK84_001119"
     CDS             complement(42070..43779)
                     /gene="hycE"
                     /locus_tag="KLK84_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HBE2008530.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(43797..44720)
                     /gene="hycD"
                     /locus_tag="KLK84_001120"
     CDS             complement(43797..44720)
                     /gene="hycD"
                     /locus_tag="KLK84_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HBE2008531.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(44723..45418)
                     /locus_tag="KLK84_001121"
                     /pseudo
     CDS             complement(44723..45418)
                     /locus_tag="KLK84_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="catalyzes the oxidation of formate to carbon
                     dioxide and molecular hydrogen; formate hydrogenlyase
                     comprises of a formate dehydrogenase, unidentified
                     electron carriers and a hydrogenase (subunit 3);
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
     gene            complement(45587..46198)
                     /gene="hycB"
                     /locus_tag="KLK84_001122"
     CDS             complement(45587..46198)
                     /gene="hycB"
                     /locus_tag="KLK84_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HBE2008532.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(46323..46784)
                     /gene="hycA"
                     /locus_tag="KLK84_001123"
     CDS             complement(46323..46784)
                     /gene="hycA"
                     /locus_tag="KLK84_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HBE2008533.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLYH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            46996..47346
                     /gene="hypA"
                     /locus_tag="KLK84_001124"
     CDS             46996..47346
                     /gene="hypA"
                     /locus_tag="KLK84_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBE2008534.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            47350..48222
                     /gene="hypB"
                     /locus_tag="KLK84_001125"
     CDS             47350..48222
                     /gene="hypB"
                     /locus_tag="KLK84_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HBE2008535.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            48213..48485
                     /gene="hypC"
                     /locus_tag="KLK84_001126"
     CDS             48213..48485
                     /gene="hypC"
                     /locus_tag="KLK84_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HBE2008536.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            48485..49606
                     /gene="hypD"
                     /locus_tag="KLK84_001127"
     CDS             48485..49606
                     /gene="hypD"
                     /locus_tag="KLK84_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HBE2008537.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            49603..50613
                     /gene="hypE"
                     /locus_tag="KLK84_001128"
     CDS             49603..50613
                     /gene="hypE"
                     /locus_tag="KLK84_001128"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HBE2008538.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            50687..52765
                     /gene="flhA"
                     /locus_tag="KLK84_001129"
     CDS             50687..52765
                     /gene="flhA"
                     /locus_tag="KLK84_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HBE2008539.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(52802..53155)
                     /locus_tag="KLK84_001130"
     CDS             complement(52802..53155)
                     /locus_tag="KLK84_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HBE2008540.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
BASE COUNT        12633 a        14308 c        13619 g        12638 t
ORIGIN      
        1 tcagcttacg cttttactct catttacgct gctgaggctg gtatatgttg catttttagt
       61 ttagagttta cttaatttag aaaatactta aataaatatg actgaactcg cgcaattaca
      121 ggccagtacc gaacaggcag cggccttatt gaaagcaatg agccacccta aacggttgct
      181 gattctgtgc atgcttagcg gttcccccgg caccagcgcg ggagagctga cgcgcattac
      241 cggactgagc gcctctgcga catcacagca tctcgctcgt atgcgggacg aagggcttat
      301 caacagccaa cgggatgccc aacgcattct atattccatt aaaaatgagg cggtaaatgc
      361 cattatcgcc accctgaaaa atgtctattg tccgtaagga gtcactatgg ctttgacaac
      421 catttcgccg catgatgcac aagaattaat cgcacgcggc gcaaagttaa tcgatattcg
      481 tgacgctgat gaatatcttc gtgaacatat tcctgaagca gatctggctc cattatccgt
      541 gctggaacag tcaggtcttc cacctaaatt acgtcgcgag caaattatat tccactgcca
      601 ggcaggtaaa cgcaccagta ataacgccga taaattagca gcgattgccg cccccgcaga
      661 aatattttta ctcgaagatg ggattgatgg ctggaaaaga gctggattgc cagtagcggt
      721 aaataaatct caacccttgc cgttaatgcg ccaggtgcag atcgctgcgg gtggtttaat
      781 attaatcggc gttgtactgg gttataccgt aaatagcggt ttcttcttat taagtggctt
      841 tgttggtgcc gggttactgt ttgcaggaat cagcggtttt tgtggaatgg caaggttgtt
      901 agataagatg ccgtggaacc aacgagcttg agaagcgacg ccggacgcgc cctagcagcg
      961 acatccggcc tcagtaatta gatcaggaaa tcgtcgagag atttaccttc tgccagcgcc
     1021 tgagcaattg gcttcggtgt acggccctga ccggtccagg ttttagtttc accgttaaca
     1081 tcagtgaatt tatatttcgc cggacgcggc tggcgttttt taccagcacg tggtgcagca
     1141 gcagagctat tacccaataa ctcttccggg ttaattccgt cagctttcat cagctccagc
     1201 caggtgctaa ttttttcctg acgctcagcc agttcacgct gctgctgttc ttcttcttca
     1261 cgtctttctt tagtgacaac cctgaatttt tcgagcattt cttcaagaac gtcaatggag
     1321 aattcgcgag ccatcgcacg gagggtgcga atgttattta aactttgtaa cattacggac
     1381 ataataaaga aaacctttta acgccaaaac aaaaaagtat ttcgtcgcgt agtgtaagcg
     1441 atcacttatt atatttccac aaaatttaat ctatttacag tcctgcgcga gattatttca
     1501 gctgttattt tctgaaactc tcagaataat ttcaagatta tatattagcc attttgatga
     1561 gtaatttagg ttattggtga acagagtggc gctctctgct cgctaactcc gcaagcctga
     1621 caaaaccgaa gtgaataaaa atgcaatata acagaataaa agaaaacacc cctcactggt
     1681 gctttctgct gttaatgtac attttctggc atcaaaaatg ctgtaaatga cccggtcacg
     1741 acaaacaccg aattctctac gcttatagcg ttacctcccc cccaaacttt cattagtgaa
     1801 attaaaggca agatcatatc taattgattt aattgaaaat aaatcatatc ggcatgtaaa
     1861 aatccacaat gtacaaaaaa cgagccgtta cggaatattt tatctacaaa aactaactaa
     1921 ataaaaattt ttcactaatt gattagtcat agacagcgat atacgctatg caaaaatgca
     1981 gatggcaatg agatccactg ctttcatctc cattaacatc ccattacgct tttattaagg
     2041 agcattagca tgttctcacc gcagtcacgc ttgcgtcatg cagttgcaga tacgttcgcg
     2101 atggttgttt actgttctgt cgtgaacatg tgtattgaag ttttcctctc cggaatgagc
     2161 ttcgaacagt ctttttattc cagattggta gcgattccgg tgaacatctt aattgcatgg
     2221 ccatacggta tgtaccgcga tctgtttatg cgcgcggcac gcaaagttag cccgtcgggc
     2281 tggataaaaa atctggctga tatcctggct tatgtgacgt tccagtcacc ggtgtatgtg
     2341 gcgatcttgt tagtggtggg cgcagactgg catcagatta tggcggcggt cagttcaaac
     2401 atcgttgttt cgatgttgat gggggcggtt tatggctact tcctcgatta ttgccgccga
     2461 ctgtttaaag tcagccgtta ccagcaggta aaagcctgac tcttcttttc gcgactggca
     2521 tcgccagtcg cgtctttaac tggcttcgcc aaataaacca ttcaaataac gttcaagcgc
     2581 aacacgcgaa ctaaagccat gcgggatacc gtaatgctcg tgtcgctcgc ctgctaaata
     2641 gagcggaaat tgctggtaat gatcgcaagt tttaatatcg gaagcgggtt cagccagtgt
     2701 tgaactgcgt tcttttaatg ttggatgaat taccagagcg gtacgtccca ttcgcgcttc
     2761 gcgattaaca tagacataat tttccccacg gcggtaacca tacgctttct gggttaccac
     2821 atcgacagta aaaccgactt tttcaagtac gcgcgccacc tcgtctggtc gtaaatacat
     2881 atttgatcct cgttattttc aaccgcgggc ctaccttacc tgattgcgca ttcacaacgc
     2941 tttcagaaaa gtccataaag ccgcgatgaa cctcagttaa gaaaattatg gtctacactg
     3001 aaaattacat cgaattctaa tgggggatca tatgtataac cgaccgaacc gcaacgacgt
     3061 tgatgatggc gtgcaggata tccagaatga tgtcaatcaa ttagctgaca gcctcgaatc
     3121 tgtattgaag tcctggggca gcgacgccaa aggggaagct gaagccgcac gcagtaaagc
     3181 tcaggcgttg ctgaaagaaa ccagggcgcg aatgcatggt cgtactcgcg tccagcaagc
     3241 cgcgcgcgat gccgttggct gagcggattc ttttgttcgt gaaagaccct ggtgtagcgt
     3301 gggtacagca gctgcggtag gtatttttat cggcgcactg ttaagcgtgc gcaaatcgta
     3361 gtgcaaaaat gataataaat acgcgtcttt gaccccgaag cctgtcttcg gggtttcttt
     3421 ttgcctggtg aatcacaaaa atccccctcc cccgtcacgc tcatatccag ggtaatttcg
     3481 atcactattt gctatatatt gtgtggttga atcttttttc aactacatct agtatctctg
     3541 tatcaacaga gagacaaccc gacgcgtatc atcgcgccgt atcttcattt taaacggaaa
     3601 tacgaatcat gcgcataact atttatactc gtaacgattg cgttcagtgc cacgccacca
     3661 aacgggcgat ggaaaaccgg ggctttgatt ttgaaatgat taatgtcgat cgcgttcctg
     3721 aagcagcgga agcgttgcgt gctcagggct ttcgtcagtt gccggtagtg attgctggcg
     3781 atcttagctg gtctggtttc cgtccggaca tgattaaccg tctgcatcca gcgccacacg
     3841 cggccagtgc atgagccagc tcgtctactt ctccagcagc tccgaaaaca cgcagcgttt
     3901 tatcgaacgt ttaggtctgc ccgcggtgcg catcccgctc aatgagcggg aacggattca
     3961 ggtagacgag ccttacatcc tgatcgtgcc ctcttacggc ggcggcggta cggctggcgc
     4021 ggtgccacga caggtaattc gctttttaaa cgacgagcac aaccgggcgt tgcttcgcgg
     4081 cgttattgct tctggtaatc gcaactttgg tgaggcgtat ggccgcgccg gagatgtgat
     4141 tgcccggaaa tgcggcgtgc cgtggctgta ccgttttgaa ctcatgggta cgcaaagcga
     4201 tatcgaaaac gttcgtaaag gagtaaccga attttggcaa cgacaaccgc agaatgcctg
     4261 acgcaggaaa cgatggatta ccacgcgctg aatgcgatgc ttaatcttta cgatagcgca
     4321 ggtcgcattc agttcgataa agaccgccag gccgttgacg cctttattgc gacgcatgtg
     4381 cgtccgaaca gtgtgacctt cagtagccag cagcagcgcc tgaactggct ggtcaacgaa
     4441 ggttactatg atgaaagcgt tcttaatcgc tactctcgcg actttgtcat tacgctgttt
     4501 gcccacgcac acaccagcgg ttttcgtttc cagacattcc tcggggcatg gaagttttac
     4561 accagctata cgttgaagac attcgacggt aaacgttatc tggaagattt tgccgatcga
     4621 gtcacgatgg tggcgctgac gctggcacaa ggcgatgaga cgctggcgtt gcaactgacc
     4681 gatgaaatgc tgtcaggacg ctttcagcca gccacgccaa cattcctcaa ctgcggtaag
     4741 cagcagcgcg gcgaactggt ttcctgtttt ttgctgcgta ttgaagacaa tatggagtcg
     4801 attggtcggg cggtaaattc cgcactgcag ctgtcgaaac gcggcggcgg cgtagcattt
     4861 ttgctgtcga atctgcggga agcgggcgcg ccaattaaac gtattgaaaa tcaatcttct
     4921 ggcgtaattc cggtgatgaa aatgttggaa gacgcatttt cctatgccaa ccaactcggc
     4981 gcgcgtcagg gggcaggtgc agtctattta catgctcatc atcccgatat tctgcgtttt
     5041 ctcgacacga aacgggaaaa tgccgacgaa aaaatccgca ttaaaacact gtcgctgggc
     5101 gtggtgatcc cggatatcac tttccatctg gcaaaagaga atgcgcagat ggcgctgttt
     5161 tcgccctatg acgttgagcg agtttatggc aagccgtttg ccgatatcgc cattagcgaa
     5221 cactatgacg aactggttgc cgatgaacgc atccgcaaaa aatacctcaa cgcccgtgat
     5281 ttcttccagc gactggcaga aatccagttt gagtccggct atccctacat catgtatgaa
     5341 gacacagtaa accgtgctag ccctatcgcc gggcgcataa atatgagtaa tctctgctca
     5401 gaaattttgc aggttaacag cgcctcagag tatgacgaga atctcgacta tgcccgcaca
     5461 ggccatgata tttcctgcaa tttaggttcg ttgaatattg cgcacaccat ggattccccc
     5521 gatttttccc gcacggtaga gactgccgtg cgcggtttaa cggcggtgtc agatatgagt
     5581 catatccgca gcgtgccgtc catcgaagcc ggaaatgccg cctcgcacgc catcggactg
     5641 gggcagatga atttacatgg ttatctggcg agggaaggca tcgcttatgg ttcgccggaa
     5701 gccctggatt tcaccaatct ctatttctat accatcacct ggcacgcact gcgtacctcg
     5761 atgttgctgg cgcgcgaacg cggtgaaacc ttcgccgggt tcaaacagtc acgctatgcc
     5821 agtggtgaat atttcagcca atatctgcaa ggtaactggc agccgaaaac ggcgaaagtt
     5881 ggcgaactgt ttgcccgcag ctgtattacg ttacctaccc gtgagatgtg ggcgcaactg
     5941 cgcgacgacg tgatgcgcta cggcatatac aaccagaatc tccaggcggt gccgccaacc
     6001 gggtctatct cttatatcaa ccatgctacg tcgagtattc atccgattgt ggcgaaagta
     6061 gagatacgca aagagggcaa aacaggacgc gtttactacc ctgccccgtt tatgactaac
     6121 gaaaatctgg cgctgtatca ggacgcttac gaaattggcg cagaaaagat catcgacacc
     6181 tacgcggaag cgactcgcca tgtcgatcag gggctgtcgc tgacgctgtt tttccccgat
     6241 accgccacca ctcgcgatat caacaaagcg cagatttacg cctggcgcaa aggtatcaaa
     6301 acgctctatt acatccgcct gcgtcagatg gcgctggaag gcactgaaat tgaaggctgc
     6361 gtctcctgtg cactttaagg aatatctatg aaactctcac gtatcagcgc catcaactgg
     6421 aacaagatat ctgacgataa agatctggag gtgtggaatc gcctgaccag caatttctgg
     6481 ctaccagaaa aggtgccgct gtcgaacgat attcccgcct ggcagacatt aactgtcgta
     6541 gaacaacaac tgacgatgcg cgtttttact ggcctgacgc tgctcgacac gctgcaaaat
     6601 gttatcggcg cgccttcgct gatgcccgat gcgctcacgc ctcatgaaga ggcggtatta
     6661 tcgaatatca gctttatgga agcggttcat gcccgctctt acagttcgat tttctcgacg
     6721 ctgtgccaga ccaaagatgt cgatgccgcc tacgcctgga gtgaagaaaa cgcaccgttg
     6781 cagcgaaaag ctcagattat tcagcaacat tatcgcggtg atgatccgct gaaaaagaaa
     6841 atcgccagtg tgtttcttga atcttttttg ttctattccg gtttctggct gccgatgtat
     6901 ttttccagcc gcggaaagct gaccaatacc gcggacctga tccgtctgat tatccgcgat
     6961 gaagcagtcc acggttacta cataggctat aaatatcaga aaaacatgga aaagatatct
     7021 ctgggacaac gtgaagagtt gaagagtttc gccttcgatt tgttgctgga actctacgac
     7081 aacgagttgc aatacaccga tgagctgtac gccgaaaccc cgtgggctga cgatgtgaaa
     7141 gcgtttctct gttacaacac caataaggct ttgatgaatc tgggctacga accgttattt
     7201 cccgcagaaa tggcggaagt gaatccggca atcctcgccg cgctttcgcc gaatgccgat
     7261 gaaaatcacg atttcttttc cggttcaggc tcctcttatg tgatggggaa agcggttgaa
     7321 acagaagatg aagactggaa tttctgaggg tgttattttc aaaaatatca ctacccgcag
     7381 cagggaaata attcccgcca aatagctttt tatcacgcaa atattctgta gtgatctaca
     7441 ctgatactct gttgcattat tcgcctgaaa ctacaatatt caggcgtttt ttcgctatct
     7501 atgacaaaaa atatcaactt ttctcgattt gctctcagcc cttatatcac ggaaaattcc
     7561 ggcgatttgc tcgcatcaat attcatgcca catttgccct caggggttgc ctcagattct
     7621 cagtatgtta gggtagaaaa aagtgactat tcccattggg taatatatcg acataaacaa
     7681 ataaaggaat cattctattg catggccatt aaattaaaaa ttaaaaatct ttataaaata
     7741 tttggtgagc atccacagcg agcgttcaaa tatatcgaac aaggactttc aaaagaacaa
     7801 attctggaaa aaactgggct atcgcttggc gtaaaagacg ccagtctggc cattgaagaa
     7861 ggcgagatat ttgtcatcat gggattatcc ggctcgggta aatccacaat ggtacgcctt
     7921 ctcaatcgcc tgattgaacc cacccgcggg caagtgctga ttgatggtgt ggatattgcc
     7981 aaaatatccg acgccgaact ccgtgaggtg cgcagaaaaa agattgcgat ggtcttccag
     8041 tcctttgcct taatgccgca tatgaccgtg ctggacaata ctgcgttcgg tatggaattg
     8101 gccggaatta atgccgaaga acgccgggaa aaagcccttg atgcactgcg tcaggtcggg
     8161 ctggaaaatt atgcccacag ctacccggat gaactctctg gcgggatgcg tcaacgtgtg
     8221 ggattagccc gcgcgttagc gattaatccg gatatattat taatggacga agccttctcg
     8281 gcgctcgatc cattaattcg caccgagatg caggatgagc tggtaaaatt acaggcgaaa
     8341 catcagcgca ccattgtctt tatttcccac gatcttgatg aagccatgcg tattggcgac
     8401 cgaattgcca ttatgcaaaa tggtgaagtg gtacaggtcg gcacaccgga tgaaattctc
     8461 aataatccgg cgaatgatta tgtccgtacc ttcttccgtg gcgttgatat tagtcaggtg
     8521 ttcagtgcga aagatattgc ccgccggaca ccgaatggct taattcgtaa aactcctggc
     8581 ttcggcccac gttcggcact gaaattattg caggatgaag atcgcgaata tggctacgtt
     8641 atcgaacgcg gtaataagtt tgtcggcgca gtctccatcg attcgcttaa aaccgcgtta
     8701 acgcagcagc aaggtcttga tgcggcgctg attgatgcgc cgttagcagt cgatgcacaa
     8761 acgcctctta gcgagttgct ctctcatgtc ggacaggcac cctgtgcggt gcccgtggtc
     8821 gacgaggacc aacagtatgt cggcatcatt tcgaaaggaa tgctgctgcg cgctttagat
     8881 cgtgaggggg taaataatgg ctgatcaaaa taatccgtgg gataccacgc cagcggcgga
     8941 cagtgccgcg caatccgcag acgcctgggg tacaccgacg actgcaccgg ctgacggcgg
     9001 tggtgctgac tggctgacca gtacgcctgc gccaaacgtc gagcatttta atattctcga
     9061 tccgttccat aaaacgctga tcccgctcga cagttgggtc actgaaggga tcgactgggt
     9121 tgttacccat ttccgtcccg tcttccaggg cgtgcgcgtt ccggttgatt atatcctcaa
     9181 cggtttccag caattgctgc tgggtatgcc agcgccagtg gcgattatcg ttttcgctct
     9241 catcacctgg cagatttccg gggtcggaat gggcgtggcg acgctggtct cgctgattgc
     9301 catcggcgca atcggtgcct ggtcgcaggc aatggtgact ctggcgctgg tgttaactgc
     9361 cctgctgttc tgtatcgtca tcggtttgcc gttggggata tggctggcga gaagtccgcg
     9421 agcggcgaaa attattcgtc cactgcttga tgccatgcag accacgccag cgtttgttta
     9481 tctggtgcca atcgtcatgc tatttggtat cggtaacgtg ccgggcgtgg tggtgacgat
     9541 catctttgcg ctgccgccga ttatccgtct gaccattctg gggattaacc aggttccggc
     9601 ggatctgatt gaagcctcgc gctcattcgg tgccagcccg cgccagatgc tgttcaaagt
     9661 tcagttaccg ctggcgatgc cgaccattat ggcgggcgtt aaccagacgc tgatgctggc
     9721 cctttctatg gtggtcattg cctcgatgat tgccgtcggc gggctgggtc agatggtact
     9781 tcgcggtatc ggtcgtctgg atatggggct tgccaccgtt ggcggcgtcg ggattgtgat
     9841 cctcgccatt atcctcgatc gcctgacgca ggccgttggg cgcgactcac gcagtcgcgg
     9901 caaccgtcgc tggtacacca ctggccctgt tggtctgctg acccgcccat tcattaagta
     9961 actctgcact tgcccggtgg cgccgggcat tatcaccctg ccaaaaaaag gaataacaat
    10021 gcgacatagc gtactttttg cgacagcgtt tgccacgctt atctctacac aaacttttgc
    10081 tgccgatctg ccgggcaaag gcattactgt taatccagtt cagagcacca tcactgaaga
    10141 aaccttccag acgctgctgg tcagtcgtgc gctggagaaa ttaggttata ccgtcaacaa
    10201 acccagcgaa gtggattaca acgttggcta cacctcgctt gcttccggcg atgcaacctt
    10261 caccgccgtg aactggacgc cactccatga caacatgtac gaagctgccg gtggcgataa
    10321 gaaattttat cgtgaagggg tatttgttaa cggcgcagca cagggttacc tgatcgataa
    10381 gaaaaccgcc gaccaataca aaatcaccaa catcgcacaa ctgaaagatc cgaagatcgc
    10441 caaactgttc gataccaacg gcgacggtaa agcggattta accggttgta accctggctg
    10501 gggctgcgaa ggtgcgatca accaccagct tgccgcgtat gaactgacca acaccgtgac
    10561 tcataatcag gggaactacg cagcgatgat ggccgacacc atcagtcgct acaaagaggg
    10621 caaaccggtg ttttattaca cctggacgcc gtactgggtg agtaatgagc tgaagccagg
    10681 gaaagatgtg gtctggttgc aggtgccgtt ctccgcactg ccgggcgata aaaatgccga
    10741 taccaaactg ccgaatggcg cgaattacgg cttcccggtc agcaccatgc atatcgttgc
    10801 caacaaagcc tggaccgaga aaaatccggc ggcagccaaa ctgtttgcca ttatgcagtt
    10861 gccagtggcc gatattaacg cccagaacgc cattatgcat gacggcaaag cctcagaagg
    10921 cgatattcag ggccacgttg atggctggat caaagcccac cagcagcagt tcgatggctg
    10981 ggtgaatgag gcgctggcag cgcagaagta atttttattc gggcggataa ggcgttttcg
    11041 cctcatccgc cgctctgtac acaatgcctg atgcgacgct gacgcgtcct atcatgccta
    11101 caaaccacat cgcacatttc aacaagctat ctttcatccc atattcatca acatccgcta
    11161 ttattgattt ccagcttaat catcacctga tgaacaaaaa taatgactaa acctaatcat
    11221 gagcttagcc cggcgctgat cgtgctgatg tctatcgcca ccggtctggc ggtagccagt
    11281 aactattacg cccagccatt gctcgacacc atcgcgcgta acttttccct ttccgccagt
    11341 tcggcaggct ttattgttac cgccgcgcag ttgggctatg ccgcaggtct actgtttctt
    11401 gttcccctcg gtgatatgtt tgaacgccgc cgcctgattg tctcgatgac cttactggca
    11461 gcgggcggta tgttaatcac tgccagcagt cagtcactgg cgatgatgat cctcggtacg
    11521 gcattaaccg gtttattctc agtcgtggca caaattctgg ttccgctggc agcgacgctg
    11581 gcttcaccgg ataaacgcgg caaagtggtt ggcaccatta tgagcggtct gctgttgggg
    11641 atattgctgg cacgaaccgt tgccgggtta ctggcaaacc tcggcggctg gcgcaccgtc
    11701 ttttgggttg cttcggtgtt aatggcactg atggcgctgg cattatggcg tggtctgcca
    11761 caaatgaaat cagaaaccca cctcaactac ccacagttgt tgggttccgt tttcagcatg
    11821 ttcatcagcg ataagatcct gcgcacccgc gcgttgctgg gctgcatgac ctttgccaac
    11881 ttcagcattc tctggacctc aatggccttt ttgcttgccg ctccaccttt taactacagc
    11941 gatggtgaca ttggtctgtt tgggcttgcg ggagctgccg gagcgttggg cgctcgtccg
    12001 gcgggcggtt ttgccgacaa gggcaaatcg caccacaact ttcggtctgc ttctgctgtt
    12061 actttcatgg ctggcaatct ggtttggtca cacttccgta ctggcgttga ttatcggcat
    12121 tctggtgctg gacctcaccg tacagggcgt gcatatcact aaccagacgg taatttatcg
    12181 aatacatcct gatgcgcgta atcgcctgac cgcaggttac atgaccagct actttattgg
    12241 cggtgccgcc ggttcgctaa tttcagcctc agcctggcaa catggcggtt gggctggcgt
    12301 ttgtctggct ggcgcgacga ttgccctggt taacttactg gtctggtggc gaggttttca
    12361 tcgtcaggaa gccgcaaatt aagcaatcag caaccgtttt cgtggcttta cacttataag
    12421 ggtgttaaga agcccatcag tctgataagg ttaagatatt cattcagtct atttataata
    12481 ttaacaatcg ttaagcgtac actctatgga aagccctact ccacagcctg ctcctggttc
    12541 ggcgaccttc atggaaggat gcaaagacag tttaccgatt gttattagtt atattccggt
    12601 ggcctttgcg ttcggtctga atgcgacccg tctgggattc tctcctctcg aaagcgtttt
    12661 tttctcctgc atcatttatg caggcgcgag ccagttcgtc attaccgcga tgctggcagc
    12721 cgggagtagt ttgtgggttg ctgcactgag cgtcatggca atggatgttc gccatgtttt
    12781 gtatggcccg tcactgcgta gccgtattat tcagcgtctg caaaaatcga aaaccgccct
    12841 atgggcgttt ggcctgacgg atgaggtttt tgccgccgcc accgcaaaac tggtacgcaa
    12901 taatcgccgc tggagcgaga actggatgat cggcattgcc ttcagttcat ggtcatcgtg
    12961 ggtatttggt acggtaatag gggcattctc cggcagcggc ttgctgcaag gttatcccgc
    13021 cgttgaagct gcattaggtt ttatgcttcc ggcactcttt atgagtttcc tgctcgcctc
    13081 tttccagcgc aaacaatctc tttgcgttac cgcagcttta gttggtgccc ttgcaggcgt
    13141 aacgctattt tctattcccg tcgccattct ggcaggcatt gtctgtggct gcctcactgc
    13201 gttaatccag gcattctggc aaggagcgcc cgatgagcta tgaggttctg ctgcttgggt
    13261 tactggttgg cgcggcgaat tattgcttcc gctatttgcc gctgcgcctg cgtgtgggta
    13321 atgcccgccc aaccaaacgt ggcgcggtag gtattttgct cgacaccatt ggcatcgcct
    13381 cgatatgcgc tctgctggtt gtctctaccg caccagaagt gatgcacgat acacgccgtt
    13441 tcgtgcccac gctggtcggc ttcgcggtac tgggtgccag tttctataaa acacgcagca
    13501 ttatcatccc aacactgctt agtgcgctgg cctatgggct cgcctggaaa gtgatggcga
    13561 ttatataacc cacaagaatc atttttctaa aacaatacat ttacttcatt tgtcactgtc
    13621 gttactatat cggctgaaat taatgaggtc atacccaaat ggatagttcg tttacgccca
    13681 ttgaacaaat gctaaaattt cgcgccagcc gccacgaaga ttttccttat caggagatcc
    13741 ttctgactcg tctttgcatg cacatgcaaa gcaagctgct ggagaaccgc aataaaatgc
    13801 tgaaggctca ggggattaac gagacgttgt ttatggcgtt gattacgctg gagtctcagg
    13861 aaaaccacag tattcagcct tctgaattaa gttgtgctct tggatcatcc cgtaccaacg
    13921 cgacgcgtat tgccgatgaa ctggaaaaac gcggttggat cgaacgtcgt aaaagcgata
    13981 acgatcgccg ctgcctgcat ctgcaattaa cggaaaaagg tcacgagttt ttgcgcgagg
    14041 ttttaccacc gcagcataac tgcctgcatc aactctggtc cgcgctcagc acaacagaaa
    14101 aagatcagct cgagcaaatc acccgcaaat tgctctcccg tctcgaccag atggaacaag
    14161 acggtgtggt tctcgaagcg atgagctaac gcgtcatctc gctcaaaaat ccagatttat
    14221 aaaagaaaaa atgaccggcc agcatcgcaa catgctggcc tttttggcaa gcaggtcggc
    14281 tcagccgatg agttaagaag atcgtggaga acaatatgag cgcaaatgcg gagactcaaa
    14341 ccccgcagca accggtaaag aagagcggca aacgtaagcg tctgctcctc cttctcacct
    14401 tgctctttat aattattgcc gtagcgatag ggatttattg gtttttggta ctgcgtcatt
    14461 tcgaagaaac cgatgacgca tacgtggcag ggaatcagat tcaaattatg tctcaggtgt
    14521 ctggcagcgt gacgaaagtc tgggccgata acaccgattt tgtaaaagaa ggcgatgttc
    14581 tggtcactct cgacccgaca gatgctcgcc aggcgtttga aaaagccaaa actgcactgg
    14641 cttccagcgt tcgccaaacc caccagctga tgattaacag caaacagttg caggcgaata
    14701 ttgaggtgca aaaaatcgcc ctcgcgaaag cacaaagcga ctacaaccgc cgtgtgccgc
    14761 tgggcaatgc caacctgatt ggtcgcgaag agctgcaaca cgcccgcgac gccgtcacca
    14821 gtgcccaggc gcaactggac gtcgctattc aacaatacaa tgccaatcag gcgatgattc
    14881 tggggactaa actggaagat cagcctgccg tgcaacaggc tgccaccgaa gtacgtaacg
    14941 cctagctggc gctggagcgt acccgtattg tcagtccgat gaccggttat gtttcccgtc
    15001 gtgccgtgca accgggtgcg caaattagcc caacgacgcc gctgatggcg gtcgttccag
    15061 ccaccaatat gtgggtggat gccaacttta aagagacgca gattgccaat atgcgcatcg
    15121 gtcagccggt caccatcacc accgatattt acggcgatga tgtgaaatac accggtaaag
    15181 tggttggtct ggatatgggc acaggtagcg cgttctcact gcttccggcg caaaatgcga
    15241 ccggtaactg gatcaaagtc gttcagcgtc tacctgtgcg tatcgaactg gaccagaaac
    15301 agctggagca atacccgctg cgcattggtt tgtccacgct ggtgagcgtc aataccacta
    15361 accgtgacgg tcaggtactg gcaaataaag tacgttcaac accggtcgcg gtaagtaccg
    15421 cgcgtgaaat cagcctggca cctgtcaata aactgatcga cgatatcgta aaagctaacg
    15481 ctggctaatc cagaggtgcg tgtgatgcaa cagcaaaaac cgctggaagg cgcgcaactg
    15541 gtcattatga cgattgcgct gtcactggcg acattcatgc aggtgctgaa ctccaccatt
    15601 gctaacgtgg cgatccccac tatcgccggg aatctgggct catcgctcag ccaaggaacg
    15661 tgggtaatca cttctttcgg ggtggcgaat gccatctcga tcccgcttac cggctggctg
    15721 gcaaagcgcg tcggggaagt gaaactgttc ctttggtcca ccatcgcctt tgctattgcg
    15781 tcttgggcgt gtggtgtctc cagcagcctg aatatgctga tcttcttccg cgtgattcag
    15841 gggattgtcg ccgggccgtt gatcccgctt tcgcaaagtc tattgctgaa taactacccg
    15901 ccagccaaac gctcgatcgc gctggcgttg tggtcgatga cggtgattgt cgcgccaatt
    15961 tgcggcccga tcctcggcgg ttatatcagc gataattacc actggggctg gatattcttc
    16021 atcaacgtgc cgattggcgt ggcggtggtg ttgatgacac tgcaaaccct gcgcggacgt
    16081 gaaacccgca ccgaacggcg gcggattgat gccgtggggc tggcactgct ggttattggt
    16141 atcggcagcc tgcagattat gctcgaccgc ggtaaagagc tggactgttt ttcatcacag
    16201 gaaattatca ttcttaccgt ggtggcggtg gtggctatct gcttcctgat tgtctgggag
    16261 ctgaccgacg ataacccgat agtcgatctg tcgttgttta agtcgcgcaa cttcaccatc
    16321 ggctgcttgt gtatcagcct cgcgtatatg ctctacttcg acgctattgt tctgctgccg
    16381 cagttgttgc aggaggtcta cggttacacg gcgacctggg caggtttggc ctctgcgccg
    16441 gtagggatta ttccggtgat tctgtcgccg attatcggcc gcttcgcgca taaactggat
    16501 atgcggcggc tggtaacctt cagctttatt atgtatgccg tctgcttcta ctggcgtgcc
    16561 tatacctttg aaccaggtat ggatttttgc gcgtcggcct ggccgcagtt tatccagggg
    16621 tttgcggtgg tctgcttctt tatgccgctg accaccatta cgctgtctgg tttgccaccg
    16681 gaacgactgg cggcggcatc gagcctctct aactttacgc gaacgctggc ggggtctatc
    16741 ggcacgtcga taaccacgac catgtggacc aaccgcgagt cgatgcacca tgcgcagttg
    16801 actgagtcgg taaacccgtt caacccgaat gcccaggcga tgtacagtca actggaaggg
    16861 cttgggatga cgcaacagca ggcatcaggc tggattgccc agcagatcac caatcagggg
    16921 ctgattattt ccgccaatga gatcttctgg atgtcagccg ggatattcct cgtactgctg
    16981 gggctggtgt ggtttgctaa accgccattt ggcgcaggtg gcggcggagg cggtgcgcac
    17041 taagtacaac taagccagtt catttgaact ggcttttttc aattaattgt gaagatagtt
    17101 tactgactag atgtgcagtt cctgcaactt ctctttcggc agtgccagtt cttcgttgct
    17161 gttgatgcgt acctcacgtt ccagaatgct acgcgcaata tcctgcgctt cctgcaacga
    17221 gtgcatctgg taagtgccac actggtagac gttcagttcc gggatctgat tctgatcctg
    17281 cactttcagc acgtcttcca ttgccgcttt ccaggcatca gcaacacgct gctcatctgg
    17341 cgtaccaatc agactcatat aaaaaccggt gcggcagccc attggcgaga tatcgataat
    17401 ctctacacca ttaccgttaa gatggttacg cataaaacca gcaaacaggt gctccagggt
    17461 atggatccct ctttctggca tcacttcttt gttcggcacg cagaagcgca gatcgaacac
    17521 ggtgattgcg tcgccatgcg gggtgttcat tgttttcgcc acccgaactg caggcgcttc
    17581 catccgggta tgatcgactg tgaagctatc taacaacggc atttagccac ctccggtaat
    17641 ttttttaaaa attttctgaa ctctttcttc ccaggcgagt ctgagtatat gaaagacgcg
    17701 catttgttat catcatccct gaattcagag atgaaatttt ggccactccc gagtggcctt
    17761 tttcttttct gtcaggcgtg tttttccagc cacaccgcaa acggttcggt atcagcggtt
    17821 tccatttcct gctgacggcg ttcagatgcc tcgcgctcgg ctacaaaatc ctcttcgcgc
    17881 agaatttcca gcggctcttc acgcagcaga ttacggtagg cttctgcaaa cgctttgcct
    17941 gttccgccaa taccagtatc aatcatagac cttaagatac gggcagagaa agtcagatcg
    18001 ggattatcga agcaggcaac cagttcatca cacactttct gatacgcttc gccgccgtta
    18061 atgctatcca gcgtttgcgc gacgcgtttc agatcgcgga acagatcttt acccacctgc
    18121 agtaacggga actgcgcggt ttcgcagccg atacccagcg tcaggcccgg tttgcgacct
    18181 tcaaggatca cccggttcca gttaacgcgc gtacaggcaa gttcgctact gctcatttcc
    18241 ggtgcatcag ccagcgcaca ccagaccata aacaggtcga ggaatcgcac ctgctgttca
    18301 tctacaccaa tcggcgagaa cgggttgatg tccagcgaac gcacttcaat atattcaatg
    18361 ccgccacgta acagcgcatc agaaggtgac tcgccgctgc gggtaacgcg ttttggacga
    18421 atcggcgcat acagttcgtt ttcaatctgc agcacgttgc tattgatttg cagcctctta
    18481 ccgtctttct caataccaat cttcgcgtac tcttccgatg gcgttttgat tgcctgttta
    18541 aggcccgcta cgtactcgta aagatcgttg aaggtaatac caagattgct ttgcgattta
    18601 ttggtatagc cgagatcgct caaacgaaga gaggtcgcat acggcaggta atacataccg
    18661 cactcggttt tctcaaacgg cagcgacgtt ggttttcctt gcaggaaaga agaacaaatc
    18721 gccggagatg caccaaacag ataaggaatg acccaaccga aacgatagta attgcggata
    18781 acgcggaaat agcccgcaga aattttctct ttggcatcag cgcccgagat atcaccgcac
    18841 ttcgcttgcc agaatgccat tggcaaagag aaattgtagt gcacgccgga aatggtttgc
    18901 atcagcgcgc cgtagcgatt tttcagccct tcacgataca gcgttttaaa gcgtccggtg
    18961 ttagaagtgc cgtactgtgc cagttcgatg tcctgacctt ctgcgatgta gcatggcata
    19021 cttaacggcc acatccgctc atcgcccata ttgcgcgccg tataacgatg cagatcgcgc
    19081 ataaaggtca gcatatgttc aatatcacca tccactggtg taatgaattc cagcaatgct
    19141 tccgcaaaat cggtagtaat ccatttgtgc gtcagtgcgg aacctaatgc ttcaggatga
    19201 cctgttgttg ccagtgtgcc atcagcatta acacgcaaag tttcgcgctc cagcccacgc
    19261 tgtatcccct ttaacgcctg aggatgtttt tccagccagg ccagcgcctg tgatacgtcc
    19321 gggatcaaat tgacctcccg cctgtcaaaa tcgttttaat tagcataact gtaatggtga
    19381 ccatatgtgc aggcctacaa ttagtgccac cacatcatgc cctgaacggt cgctgctgca
    19441 actgcaacat agcgtaacgc tttaccaagg cacaaaaaaa agattactgg cccccaggag
    19501 atgcgcatcc atcccgctaa cagacacagt aaatcgccaa ccacgggcat ccagcttaat
    19561 aatagcgtga ctgcaccata gcgtttcagc cagccggtag ctttctcttg ccagcgcgat
    19621 gttttacgca atggaaagaa acgcccaagg ataacgttag ttaaccctcc aaggctatta
    19681 cccattgttg ctgttaaaac taaaacccag ggatgactga tcccggaaag caacattgcc
    19741 accagcacga cttcggagtt gccgggtaat agtgtagcgc tgagaaaact actggcaaac
    19801 aacgagaaaa gcgataacgc ttcactcaca gcaagcgaac atccacagcg tccatgcctg
    19861 ctgcacgggc cgcctgaata ccgaaatcgg catcttcaaa gaccacacac tgcgtcggtt
    19921 gcacgcccat acgctgcgcg cacaacaaaa atgtgtctgg cgcgggttta tggtgtttga
    19981 cgtgatcagc agcgacgacg gcgtcaaaat aacggcgtaa tcccaggtgc gccagcaatg
    20041 cctcagcgat ggcgctttca ctccccgttc ctacagccat tgggcgacga ccatgccaac
    20101 ttttcaccac ttcaacaaga ggaagcggct cgacactatc cagcagcata cttcttactg
    20161 cttctgtttt ttcacgcgct aacgcatgcg ggtcgagatc ggcctgattc agctcaataa
    20221 ttgcctgagc aatacgccag gtgggcgatc cattaagcgc aatcatcgcc tgaatatcgt
    20281 actgaagacc gtagtgccct aatacttcgc gccacgcttt acggtgcgta ggctccgtat
    20341 ccaggattgt gccatccata tcaaaaatta aacctgcata acgctcgtac atggtcttct
    20401 cgcaagtcga aaaatatgac gctactttag cgtaattgct tgattttgtc gctgatggcg
    20461 aaggagaaga aaattcagga aatggataaa gtagttattt agaaatgaga tatttttgaa
    20521 ggaaattttt tggagaatat ggtgcatccg ggaggattcg aacctccgac cgctcggttc
    20581 gtagccgagt actctatcca gctgagctac ggatacatcg ggaaacttac tttactgcag
    20641 attttttgat accgctacta aagccgtatc aagtaagaga tggtgcatcc gggaggattc
    20701 gaacctccga ccgctcggtt cgtagccgag tactctatcc agctgagcta cggatgcatc
    20761 gggaaactta ctttactgca gattttttga taccgctact aaagccatat caagtaagag
    20821 atggtgcatc cgggaggatt cgaacctccg accgctcggt tcgtagccga gtactctatc
    20881 cagctgagct acggatgcat cgggaaactt actttactgc agattttttg ataccgctac
    20941 taaagccgta tcaagtaaga gatggtgcat ccgggaggat tcgaacctcc gaccgctcgg
    21001 ttcgtagccg agtactctat ccagctgagc tacggatgca aatggcggtg aggcggggat
    21061 tcgaaccccg gatgcagctt ttgaccgcat actcccttag caggggagcg ccttcagcct
    21121 ctcggccacc tcaccacacg cctcttacga gtgcttcgaa gaacttgttt atcgctcatc
    21181 atcgctgcgt ggcgcacata ttactttctg agacttataa gtcaaacaat ttttcccaca
    21241 cttttatcgt ttgcacactt cacgctcaat tagtctgtaa aagcgtcaaa aagagtgttt
    21301 tatctacaga aaaacggagg tctggcagat agcagtaatg caaaaaaatg gagacttaag
    21361 ttgaatgaac gggagtaaag cgaaaagact atagagtgaa ggagaaattt tgagggtgcg
    21421 tctcaccgat aaagatgaga cgcggaaaga ttagtaactg gactgctggg atttttcagc
    21481 ctggatacgc tggtagatct cttcacggtg aacagaaact tccttcgggg catttacgcc
    21541 aatacgtacc tggttgccct ttacccctaa aactgtcacg gtgacctcat ccccaatcat
    21601 gagggtctca ccaactcgac gagtcagaat cagcattctt tgctccttga aagattaaaa
    21661 gagtcgggtc tctctgtatc ccggcattat ccatcatata acgccaaaaa gtaagcaatg
    21721 acaaacacat tacatgtaag cagtcatggc attacattct gttaaaccta agtttagccg
    21781 atatacacaa cttcaacctg actttatcgt tgtcgatagc gttaatgcga atgccgtgaa
    21841 gcgagtccac ggcattgcct gacgcttata ttattgcaat ttcgcgctga cccagccttt
    21901 cacactggct aacgctgcag gtaaggccgc agcatccgta ccaccggctt gcgccatgtc
    21961 aggacgtcca ccacccttgc cgcccacctg ctgagcgacc ataccaatca gttcccctgc
    22021 tttcacacga tctgtgacgt ccttagatac gcctgcaatc agagaaacct taccttcggc
    22081 taccgttgcc agcacgataa ttgtcgaccc cagctgattt tttaaatcgt caaccatggt
    22141 acgcaacatt ttcggctcaa caccgctaag ctcgctaacc aacagcttaa caccattaac
    22201 atcaattgcc ttactggaaa gatttgcgct ctcctgtgcg gcagcttgtt ctttaagctg
    22261 ttgtagttct ttttctaact gacgcgtacg ttccagtact gagcgcactt tatccgccag
    22321 attattgcta tcgcctttca gcagatgcgc gacttcgctt aagcgatcgc tgtctgcatg
    22381 aacggtggtg atagcgcctt ctccggttac cgcttcgata cgacgaacgc ctgcagcagt
    22441 acccgattca gagatgatgc ggaacagacc aatatcacca gtgcggctgg cgtgagtacc
    22501 gccacacagc tcggtggaga aatcgcccat gctcagcacg cgtacacgct catcatactt
    22561 ctcgccgaac agcgccatcg cacctttcgc tttcgccgct tcgagattca tgatgttggt
    22621 ttcgatcggc aaattgcgac gaatctgtgc gttcaccagg tcttcgaccg cacgaatctc
    22681 ttccggtttc atcgcttcgt tgtgtgagaa gtcgaagcgc agcaccttgt cgttaaccag
    22741 tgaacctttc tgcgatacat gagtccccag aacctggcgc agcgcagcgt gcatcaggtg
    22801 cgttgcggag tgattcagac gaatacgggc gcgacgagcc tcatcaatat cagcctgcac
    22861 agcgtcgccc actttcagag aacccgcagc aagtttaccg atgtgaccaa tcgcctggcc
    22921 gtatttctgc gtatcttcca ccgcgaagga gaagttagcg cctttcagtt cgcctttatc
    22981 accaacctga ccgccggatt ccgcatagaa tggcgtttga tccagcacga ccacagcttc
    23041 ctggcctgca ttgatggcat caaccgcttt accatcaaca aacagcgcgg tcactttgcc
    23101 gttcagttcc agatggtcat agcctttaaa ttcagatgca ctatcaacac ggatcattgc
    23161 gttgtaatcg gcaccaaagc cgctggcttc gcgcgcgcga cgacgctgct cttccattgc
    23221 tgcttcaaaa ccagcttcgt caactttgat gttgcgctca cgacaaacat cagccgtcag
    23281 gtcaaccggg aagccatagg tgtcgtacag acggaaagcc gtttcaccat ccagcgtatc
    23341 accagaaagt tttgccagct cttcatccag caacgccaga ccgcgctcca gagtacgggc
    23401 aaactgctct tcttcagtct tcagcacctg ctcaacctgc gcctgctggc gtttcaggtc
    23461 ttcacccgca gagcccataa cgtcgatcag cggaccaacc agtttgtaga agaaggtttc
    23521 tttcgcgccg agcatattgc cgtgacgcac tgcgcgacga atgatacgac gcagtacata
    23581 accacggttt tcattggacg gcattacgcc atccgcgatc aggaacgcac aagaacgaat
    23641 gtggtcagcg attacgcgca gcgatttatt gctcagatcg gtcgcgccag tgactttcgc
    23701 taccgcctgg atcaacgtgc ggaacaggtc gatgtcatag ttagagttaa cgtgttgcag
    23761 caccgcagca atacgctcca gacccatacc ggtatctaca gacggcttcg gcagcggttc
    23821 catcgtgcca tctgcctggc ggttgaactg catgaagacg atgttccaga tctcaatgta
    23881 gcggtcgccg tcttcttccg gacttcccgg agggccaccc caaatgtggt cgccgtgatc
    23941 gtagaagatt tcggtgcacg ggccgcacgg accagtgtca cccatctgcc agaagttgtc
    24001 agatgcgtat ggcgcacctt tgttatcgcc gatgcgaata atacgttcgc gcgggatccc
    24061 gacttctttt tcccagattt cgtaggcttc gtcgtcgctt tcatagacgg taacccacag
    24121 acgctctttc ggcagggcaa accatttttc gctggtcagc agttcccatg caaactgaat
    24181 ggcatcgtgt ttgaaatagt cgccgaagct gaagttgccc agcatttcga agaaggtatg
    24241 gtgacgcgcg gtgtaaccga cgttttccag gtcgttgtgt ttaccacccg cacgcacgca
    24301 gcgttgggaa gtggtagcgc gggaataatt acgcttgtcg agcccaagga acacatcctt
    24361 gaactggttc atcccggcgt tggtaaacaa caaagttggg tcgttatggg gtaccaggga
    24421 gctgctggca actacctgat gtcccttact atggaaaaag tcgagaaacg cctgacggat
    24481 ctcagcggtg ctcttgctca taattatcct gaaatcaagc taacgaaata tcgccaccag
    24541 ctccagcgtg tcttaaccgc cgggctggta actgaaaagt gggaataaga taagttttct
    24601 tgactgggaa gtaaaatacc gtatgcgttc agtcggcaaa atttcgccaa atatcctgga
    24661 tatcttccat cagatagcca cgatagagca gaaaacgctg gatcttaact ttttctgaaa
    24721 agacagttgg caaaggttca ccatattttc gcgtcgcctg atcgcgcgcc agtgcgcacc
    24781 agtcgatgtc acattcacgc atcgcttttt ctgtcgcttc gcgggaaata cctttctgat
    24841 tcagttcctg acgaatacgt gcaggtccat accctttgcg gctacggctg gcgataaagc
    24901 gcgcaacaaa tcggctgtca tcgagatagc catgttcatg gcaccaggca ataacgcgct
    24961 cgtaatcttc tgccgtagca tcaatctctt ctgggccatt tttgcccata atcggtgccg
    25021 cgagtttacg tcgcagttct tgctcactgt gatcgcgcac cgccagaatg cgtaccgcac
    25081 gatccaacag gcgagcatat gccgggcgac gggatgttga ttctgtcatg gcatatcctt
    25141 acaacttaaa aaagcaaaag ggccgcagat gcgccccagg ttttgctgat aaacagattt
    25201 aatagtcaat gagtatgcag aatgttcccg caggcccaat tcgttagagg atcgaggtga
    25261 gagtaatttt tcacgccagt gctggcctgt tcccctcacc ctaaccctct ccccaaaggg
    25321 gcgaggggac cgtccgtgca cacattatct attatcatca ataacgaacg attaaaaatc
    25381 ttcgttagtt tctgctactc cttcgctgtc atctacagag aaatccggcg ttgagttcgg
    25441 gttgctcagc agcaactcac gtactttctt ctcgatctct ttcgcggttt ccgggttatc
    25501 tttcagccag gcagttgcat tcgctttacc ctgaccgatc ttctcacctt tgtagctgta
    25561 ccacgcgcct gctttctcga tcagcttctc ttttacgccc aggtcaacca gttcgccgta
    25621 gaagttgata ccttcgccgt agaggatctg aaattcagcc tgtttaaacg gtgcagcgat
    25681 tttgttcttc accactttca cgcgggtttc gctacccacc acgttttcgc cctctttcac
    25741 cgcgccgata cgacggatgt cgagacgaac agaggcgtag aatttcagcg cgttaccacc
    25801 ggtagtggtt tccgggttac cgaacatcac accaattttc atacggatct ggttgatgaa
    25861 gatcagcagc gtgttggact gcttcaggtt acccgccagc ttacgcatcg cctggctcat
    25921 catacgtgcc gcaaggccca tgtgagagtc gccgatttcg ccttcgattt ccgctttcgg
    25981 cgtcagtgcc gccacggagt caacgacgat aacgtctact gcgccagaac gcgccagggc
    26041 gtcacagatt tccagtgcct gctcgccggt atccggctgg gagcacagca ggttatcgat
    26101 atcgacgccc agtttacgtg cgtagattgg gtccagcgcg tgttcagcat cgataaacgc
    26161 acaggtttta ccttcacgct gcgctgcggc gatcacctgc agcgtcagcg tggttttacc
    26221 ggaagattcc ggtccgtaga tttcgacgat acggcccatc ggcagaccac ctgccccaag
    26281 cgcgatatcc agtgaaagcg aaccggtaga gatggtttcc acatccatgg aacggtcttc
    26341 acccaggcgc atgatggagc ctttaccaaa ttgtttctca atctggccca gtgctgccgc
    26401 caacgctttc tgtttgtttt cgtcgatagc catttttact cctgtcatgc cgggtaatac
    26461 cggatagtca atatgttctg ttgaaacaat tatactgtat gctcatacag tatcaagtgt
    26521 tttgtagaaa ttgttgccac aaggtctgca atgcatacgc agtagcctga cgacgcaccg
    26581 catcacggtc gccgctgaag cattcccgcc gggtaatgcc ttcaccgcgg gcagtggcaa
    26641 aagcaaacca gacggtgccg acaggcttct cttcactgcc gccatccggc ccggcgatac
    26701 cactaataga cacggcataa tcagcacgag ccgctttcag tgcacctatc gccatttcca
    26761 ccacgacggg ttcactcacc gcgccatgct gcgccagcgt ctcttcgcgt acgccgatca
    26821 tctgcgcttt ggcttcgtta ctgtaggtga caaatccgcg ttcaaaccag gcggagctac
    26881 cggcaatatc ggtaatcact ttcgctaccc aaccaccggt acaagactcg gcagttgtta
    26941 cggttgcgcc acgggctttc agcgcctgcc caacctgttc gcttaactgc atcagttcac
    27001 tgtcagtcat cacaacctct gtcagtcaaa aatttatgcc ggacaagata gcactttcgt
    27061 caggaagatg gtgttggcgt tgcaatttta cgaggaggat tcagaaaaaa gctgattagc
    27121 cagagggaag ctcacgcccc cctcttgtaa atagctactg tactcgcgcc agcgccacgg
    27181 cttgtcctaa ctgccagacc gccattgcgt aatgggtgct gtggttgtaa cgggtgatgg
    27241 tgtagaagtt cggcagaccg taccagtact ggtagccggt gccaacatcc agacgcagca
    27301 ggctggcttg ttgatggttg cccagcggct gctgtggcgt taaacctgcg gcggcaagct
    27361 gcgaaatgct gtacttagtt ttgaaaccat ttggcaagcc tggagcctga ccgtttgcca
    27421 ttatcgcgac ctgatcgcct ttcacccagc cgtgcgcttt gaaatagttc gccacgctac
    27481 cgatcgcatc aaccggatcc cacaggttga tatgcccgtc gccgctgaaa tctaccgcat
    27541 attgtttgta agacgacggc ataaactgtc cgtagcccat cgccccggca aaggaacctt
    27601 tcagattgag cggatcgtcc tgctcgtcgc gcgccatcag caggaaggtt tccagctcgc
    27661 cagagaaata ctccgcgcgg cgtgggtagt taaatgacag cgttgccagc gcatcgagga
    27721 tgcgagtttt ccccatcacg cgcccccagc gggtttcaac gccgataatc ccgacgataa
    27781 tttccggcgg tacgccatac acctgccacg cgcgattcaa cgcatcttca tactgattcc
    27841 agaaaaccac accgttctgc acgttgtccg gcgtaataaa ttttttgcga taacgcagcc
    27901 atgcgccgtt cggacctgat ggcggtttca ccgatgtggt tggtgcctgg ttatccatca
    27961 gccgcagtac cgaatccaga cgcttcgcct gggagagaat ttcctgcaac tgctgacgat
    28021 cgaaaccgtg tttgttcacc attttgtcga tgaactgctg ggcattcggg ttattagcga
    28081 aatcgccgcc catctgcatc acattgtgct gcggctcaag caggaagccg ccagacggcg
    28141 ttccggtggt cgtttcagtc tcagtaggtt ttggcttgct gctacaggcg gcaagcaaca
    28201 caaaaagggg aagcaatgtt acataacgac gcttgaacat gaggggtcca tttaacagat
    28261 tcaaccaggg gcaagtatgg taaagcatca caccccgcac aaggaagcgg tagtcactgc
    28321 ccgatacgga ctttacataa ctcaactcat tcccctcgct atacttttat tcaaactttc
    28381 aaattaaaat atttatcttt cattttacga tcaaaataac acttttaaac ctttcaacct
    28441 gattagatta ggttgccgtt tggtaataaa acaataaatc ctgaaggaga gaacaatgat
    28501 agaaaccatt actcatggtg cagagtggtt tatcgggctg ttccaaaagg gcggagaggt
    28561 gtttaccggg atggtgaccg gcattcttcc gctgttgatt agcctgcagg ttatcatgaa
    28621 cgcactgatt aattttatcg gtcagcatcg tattgaacgt tttgctcaac gttgcgccgg
    28681 taaccctgtt tcccgttacc tactgttacc gtgcattggc acgtttgtct tttgcaatcc
    28741 gatgacctta agcctgggtc gctttatgcc ggaaaagtac aaacccagct actacgcggc
    28801 ggcctcttat agctgccact caatgaatgg cctcttcccc catatcaacc ctgcgaactg
    28861 tttgtttatc ttggcattgc cagcggtctg acaacgctga acctgccact tggcccactg
    28921 gcggtgagtt atctgctggt tggtctggtc accaatttct tccgcggctg ggtgaccgat
    28981 ctgaccaccg ccatttttga gaaaaagatg ggcattcaac ttgaacaaaa agttcacctg
    29041 gcaggagcaa catcatgacg cgtattcgga tcgaaaaagg aacgggtggc tggggcggcc
    29101 cgcttgagct ggaagccacg ccgggcaaaa aaatcgtcta tatcaccgcc ggtacccggc
    29161 ctgcgattgt tgacaaactg gcacaactta ctggctggca ggctattgac ggatttaaag
    29221 aaggtgaacc cgcggaggcg gaaattggtg tcgcggtaat cgactgtggc ggcacattac
    29281 gctgcggcat ctatccgaaa cgacgtattc ccaccattaa tatccactcg acgggcaagt
    29341 ccggtccgct ggcgcagtac attgtggaag atatttatgt ctctggcgta aaagaagaaa
    29401 acatcactgt agtgggtgat gcgacaccac aaccctcttc cgtgggccgt gactatgaca
    29461 ccagtaagaa aatcaccgaa caaagcgatg gtttactggc gaaggtggga atgggcatgg
    29521 ggtccgccgt tgccgtgttg tttcagtctg gtcgtgacac catcgacact gtattaaaaa
    29581 ccattctgcc gtttatggca ttcgtctcgg cgctcattgg catcattatg gcttctggcc
    29641 ttggtgactg gattgcccac ggtcttgctc cgctagcgag ccatccactg ggtctggtca
    29701 tgctagcgct catctgctcc ttcccactgc tttcaccttt cctcggccca ggcgcagtta
    29761 tcgcacaggt tatcggcgta ttgattggcg tgcagattgg tctcggcaat attccgccgc
    29821 atctggcttt accggcactg tttgccatca acgcgcaggc ggcttgcgac ttcatcccgg
    29881 tcggtttgtc gctggcggaa gcccgtcagg acacggttcg cgtcggtgtc ccttctgtac
    29941 tggtgagccg ctttttaacc ggcgcgccga ctgtactgat cgcctggttt gtctccagtt
    30001 ttatctatca atagaggctg aaacatgacc gttatttatc agaccaccat cacccgtatc
    30061 ggcgcgagtg ccactgacgc cctcagcgac cagatgctca tcacctttcg tgaaggcgcg
    30121 ccagcggacc tcgaagagta ttgcttcatt cattgccacg gcgagttgaa aggtgcactc
    30181 catcccggtt tgcaattttc actcgggcag catcgctatc cggtgaccgc tgttggcagc
    30241 gtggcggaag acaaccttcg cgaactgggt catgtcaccc tgcgcttcga tggtttaaac
    30301 gaagcggaat ttccgggcac tgtccatgtg gcaggccctg tacccgacga tatcgcgccg
    30361 ggatcggttt tgaagtttga atctgttaag gagtaaaaaa tgaatcaggt tgccgttgtc
    30421 atcggtggtg ggcaaacctt aggcgcgttc ctgtgccacg gtctggctgc cgaggggtat
    30481 cgcgtcgcgg ttgtcgatat tcagagcgac aaagccgcaa atgtggcaca agaaattaac
    30541 gccgaatatg gtgaaggtat ggcgtacggt tttggtgctg acgccactag cgagcaaagc
    30601 gttctggcgc tctctcgtgg ggtagatgaa atctttggtc gcgtggattt gctggtctat
    30661 agcgccggaa tagcaaaagc agcctttatc agcgacttcc agctcggcga ttttgaccgt
    30721 tcgctacaag tgaatctggt gggttatttc ctgtgtgccc gtgaattttc gcgtttgatg
    30781 atccgcgacg ggattcaggg tcgcattatt cagatcaact cgaaatccgg caaagtgggc
    30841 agcaaacaca actctggcta cagcgcagcg aaatttggtg gcgtcgggct gactcaatca
    30901 ctggcgctgg atctggcgga gtacggcatt acggtacatt cactgatgct cggtaacctg
    30961 ctgaaatcgc ggatgttcca gtcactgttg ccacaatacg cgaccaagct gggtatcaaa
    31021 ccggatcaag tcgagcagta ttacatcgac aaagtaccgc tcaaacgcgg ctgcgattat
    31081 caagatgtgc tgaatatgct gctgttctac gccagtccta aggcgtcgta ctgcaccgga
    31141 cagtcgatca atgtcaccgg cggtcaggtg atgttctgat caacagcgga gatccgttaa
    31201 ggatctccgt gagactatag aatgcctgat gcgctacgct catcaggcat acaggacttc
    31261 cgccactaca ttaaggaaaa gttatggtat ccgcactcat caccgtcgcc gttatcgcct
    31321 agtgtgcgca actggcctta ggcggctggc aaatttctcg ttttaaccgt gccttcgaca
    31381 cactatgcca gcaagggcgg gttggcgtgg gccgttccag cgggcgcttt aaaccgcggg
    31441 tcgtggtcgc catcgcgctg gacgatcagc agtgcatcgt cgacaccttg tttatgaaag
    31501 gactgactgt cttcgcccga ccgcaaaaaa ttcccgcaat taccggcatg catgcgggtg
    31561 atttacagcc cgatgtgatc tttccccatg atccactatc acagaatgct ctatcattgg
    31621 cgcttaaact gaaacgtgga taatttcgtt gtgaatgtta cttgcttgcg aagttatcat
    31681 tttgaaacct aaatcaggta atcacgccca tgaaacctcg tcagcgtcag gccgccattc
    31741 tggagtatct gcaaaagcag ggtaaatgct cggttgaaga attggcgcaa tactttgaca
    31801 ccacaggcac aaccattcgc aaagatctgg tcattctgga acatgccgga accgtcattc
    31861 gtacttatgg cggagtggtg ttgaataaag aggaatccga tccgcctatc gatcataaaa
    31921 cgctcatcaa cacccacaag aaagagctga ttgcagaagc tgccgttagt tttatccatg
    31981 atggcgattc gatcattctt gatgctggca gtaccgtttt gcagatggtt cccctgctct
    32041 cgcgctttaa taacatcacg gtgatgacca acagcctgca tatcgtcaat gcgctatccg
    32101 aactggataa cgaacaaact atcctgatgc caggcggaac atttcgcaaa aaatcggcct
    32161 catttcacgg gcaactggca gagaatgcct tcgagcattt caccttcgat aaattgttta
    32221 tgggcaccga cggcatcgat ctcaatgcgg gcgtaaccac gttcaacgag gtttataccg
    32281 tcagtaaggc aatgtgcaat gccgcgcgcg aagtgatttt gatggcggac tcatcaaagt
    32341 ttggccgtaa aagccccaac gtagtttgca gtcttgaaag cgtcgataag ctaattaccg
    32401 acgcaggtat cgatccggcg tttcgtcagg cgctggaaga gaaagggatc gatgtgatca
    32461 taaccggaga gagcaatgag tgaagcacta ctgaacacgg gacgtcagac gttaatgctg
    32521 gagttgcagg aagcaagccg tttaccggaa cgtctgggcg atgattttgt tcgcgccgcc
    32581 aatatcatcc tgcactgcga aggcaaagtg gtggtttcgg gaattggcaa atcgggccac
    32641 attggtaaga aaatcgccgc aacacttgcc agtaccggca caccggcttt ttttgtccat
    32701 ccggcagaag cactgcacgg cgatctgggg atgattgaaa gccgcgatgt gatgctgttt
    32761 atctcttact ccggtggcgc gaaggaactg gatctgatta ttccgcgtct ggaagataaa
    32821 tctatcgcgc tgctggcgat gaccggcaaa ccgacgtcac cgctgggcct ggcggcgaaa
    32881 gcggtgctgg atatctccgt agaacgcgaa gcctgcccga tgcaccttgc gccgacctcc
    32941 agcaccgtca ataccctgat gatgggtgac gcgctggcga tggcggtcat gcaggcgcgc
    33001 ggttttaatg aagaagattt tgcccgctcc cacccagctg gggcactggg cgctcgcttg
    33061 ctgaataaag tgcatcatct gatgcgtcgt gatgacgcta tcccacaggt agcgttaacc
    33121 gccagcgtga tggatgcgat gctggaactc agccgcaccg gtctggggct ggtggcggta
    33181 tgtgacgctc aacaacaggt acaaggcgtc tttaccgacg gcgatttacg tcgctggctg
    33241 gtaggcggcg gcgcactcac cacgccagtt aatgaagcga tgacgacagg cggcaccaca
    33301 ttacaggcac aaagtcgcgc catcgacgcc aaagaagttc tgatgaaacg caaaatcact
    33361 gccgcaccag tggtagatga aaacggcaaa ctcaccggcg caattaacct gcaggatttc
    33421 tatcaggccg ggattattta atccttcaat cccagacgtt tcgccagccg atgcaggttg
    33481 gcgacgtcag tttccagcat tcgcgcacag gctgcccagt tgtgatgatt ttgtgccagt
    33541 gcctggcgaa tagtttcacg ctggaacgct tctgtcgctt cacgcaggtt ttgcttaaca
    33601 acgagcaccg ccgccgcttc tggcggcggc aacgtcacct caggaaaagc aaaatgttgc
    33661 gcctcaagaa tcacttcatc gccgttgcgg gtggctctcg ccagcactac cgcccgatga
    33721 atagcatgtt ccagttcgcg cacgtttccc ggaaaactgt agtgttgcag taaatttcgc
    33781 gccccggcac ttaataccac gcgggagagc ccctgccgca aacgacactg ctcgcagaaa
    33841 taccccgcca gcagaatgac atcatcgccc cgctcacgca gcggcggcac cgaaagtgga
    33901 aacacgctca ggcgatgaaa caaatcggcg cggaatcgcc ctgccagcac ctcttcgcgt
    33961 aagtcgcggt tagtcgccgc cagcacgcgc acatcgaccc gcaaactacg gtcatcgcca
    34021 acgcgctgaa tatcgccata ctgcaacacc ctcagcagct tggcctgcaa tgccaacgac
    34081 aactcgccga tctcatcgag aaacagagta ccgttatccg ccatttcaaa cttcccgctg
    34141 cggttactga tagcgccagt aaacgctcct ttcacatgcc cgaacaactc actttccgcc
    34201 acactttccg gcagtgcggc acagttgaga tagaccagcg gattcaccgc ccgtggcgag
    34261 gcttcatgaa tcgctttcgc caccagctcc ttaccggttc ccgtctcacc gctgatcaga
    34321 acgttgagat cggacgccgc cacaatctca atctcttttt tcagttgcgt catgccaggg
    34381 gacaagccaa tcatctgcgt ctgtttcacc gcttcaaacg gcgtggcatc gcctggcagc
    34441 atattctggc tttccagttg ttcaatcagc aacgcattgc ttaacgctcc cgccgccagc
    34501 gcggcaatca gccgtaactc ttcgtcgctg aaaacatcga actgatcggg ctgcatcccg
    34561 tcgagcgtca gtgcgccgat caggttttgt ccgacaaaca atggcagacc aacgcaggcg
    34621 tgaaccttca gactctcctg cccaggaatc aaaccgtcat agggatcggg caattcgctg
    34681 tctgcgggaa agcgcaccac atccccggcg cgggcaatcg cttccagccg tggatgccct
    34741 tccagcgcaa agcgtctacc gagtacatcc tttgccagac cgtcgatggc aagcggaata
    34801 aactgccgcg aatcgtaacg tagcaacgca gacgcatcgc actccagcac ctgacgtagc
    34861 gtggtgatca ggcgctgaaa acgatcctga tgaccaatcc cacgctgcaa ttcgatggcg
    34921 atattcgcca gcacatcaac ggaaaaactc atctttgcct cactgtcaat ttgactatag
    34981 atattgtcat atcgaccatt tgattgatag tcattttgac tactcattaa tgagcataat
    35041 tttatttata gagtaaaaac aatcagataa gaaactggca cgcaatctgc aattagcaag
    35101 acatcttttt agaacacgct gaataaattg aggttgctat gtctattgtg gtgaaaaata
    35161 acattcattg ggtgggtcaa cgtgactggg aagtgcgtga ttttcacggc acggaatata
    35221 aaacgctgcg cggcagcagc tacaacagct acctcatccg cgaagaaaaa aacgtgctga
    35281 tcgacaccgt cgaccataaa ttcagccgcg aatttgtgca gaacctgcgt aatgaaatcg
    35341 atctggcgga tatcgattac atcgtgatta accatgcaga agaggaccac gccggggcgc
    35401 tgaccgaact gatggcgcaa attcccgata cgccgatcta ctgtactgcc aacgctatcg
    35461 actcgataaa tggtcatcac catcatccgg agtggaattt taatgtggtg aaaactggcg
    35521 acacgctgga tatcggcaac ggcaaacagc tcatttttgt cgaaacacca atgctgcact
    35581 ggccggacag catgatgact tacctgacag gcgacgcggt gctgttcagt aacgatgctt
    35641 tcggtcagca ctactgcgac gagcatctgt tcaacgatga agtggatcag acggagcttt
    35701 tcgagcagtg ccagcgttac tacgccaata tcctgacgcc gttcagccgc ctggtaacgc
    35761 cgaaaattac cgagatcctg ggctttaact taccagtcga tatgatagcg acctcccacg
    35821 gcgtggtctg gcgcgataac ccgacgcaaa ttgtcgagct gtacctgaag tgggccgctg
    35881 attatcagga agacagaatc accattttct acgacaccat gtcgaataac acccgcatga
    35941 tggctgacgc tatcgcccag ggaattgcgg aaaccgaccc acgcgtggcg gtgaaaattt
    36001 tcaacgtcgc ccgaagcgat aaaaacgaaa tcctgaccaa tgtcttccgc tcaaaaggcg
    36061 tgctggtcgg cacttcgacg atgaataacg tgatgatgcc gaaaatcgcc gggctggtgg
    36121 aggagatgac cggattacgc ttccgtaaca aacgcgccag tgctttcgga tctcacggct
    36181 ggagcggcgg tgcgatggat cgcctttcca cgcgcctgca ggatgcgggt ttcgaaatgt
    36241 cgctcagcct gaaagccaaa tggcgaccag accaggacgc tctggagtta tgccgtgaac
    36301 acggtcgcga aatcgcccgt cagtgggcgc tcgcgccgct gccgcagagc acggtgaata
    36361 cggtagttaa agaagaaacc tctgccacga cgacggctga cctcggccca cgaatgcagt
    36421 gcagcgtctg ccagtggatt tacgatccgg caaaaggcga gccaatgcag gacgttgcgc
    36481 caggaacgcc gtggagtgaa gtcccggata acttcctctg cccggaatgc tccctcggca
    36541 aagacgtctt tgacgaactg gcatcggagg caaaatgagc aacggcattg tgatcatcgg
    36601 ttcgggcttc gccgcccgcc aactggtgaa aaatattcgc aaacaggacg ccactattcc
    36661 attaaccctg attgccgccg acagcataga tgagtacaac aaacccgacc tcagccatgt
    36721 tatcagtcag gggcaacgtg ccgacgacct tacccgccag acggcgggtg aatttgccgg
    36781 gcagtttaat ctgcgcctgt ttccgcacac ctgggtgacg gatatcgatg ccgaagccca
    36841 tgtggtgaaa agccagaata atcagtggca atacgacaag ttagtgctgg caaccggtgc
    36901 cagcgccttt gtcccgcctg tgcctgggcg tgagttaatg ctgacgttaa atagtcagca
    36961 agagtatcgc gcctgtgaaa cgcaactgcg ggatgcccga cgcgtgttga ttgtcggcgg
    37021 tggtttgatt ggtagcgaac tggcgatgga tttttgtcgt gcaggcaaag cggtcacgct
    37081 aatcgacaac gctgccagta ttctggcgtc attaatgcca ccagaagtaa gcagccgctt
    37141 gcagcatcgg ttgacggaga tgggcgttca tctgctgttg aaatctcagt tgcaggggct
    37201 ggaaaaaacg gattctggca ttctggcaac gctggaccac cagcgtagta tcgaagtgga
    37261 tgcggtaatt gccgccaccg gactgcgccc ggaaacagcc ctggcacgac gcgccgggct
    37321 gacgattaat cgcggcgttt gcgtcgatag ttatctgcaa accagtaatg ccgatattta
    37381 cgcgctgggc gattgcgcgg aaattaacgg tcaggtattg ccgttcctgc agccgattca
    37441 acttagcggg atggtgctgg caaaaaatct tctcggcaat aacacgccgc tgaaactacc
    37501 ggcgatgctg gtgaaaatca aaacgccgga attaccgctg catctggcag gcgaaaccca
    37561 gcgtcaggat ttacgctggc agattaatac cgaacgccag ggaatggtgg cgcgcggcgt
    37621 tgacgatgct gaccagcttc gcgcctttgt ggtcagtgag gatcggatga aagaggcatt
    37681 tgtattgttg aaaacgttgc cggtgtaggt gggctactgt gcctaaaatg tcggatgcga
    37741 cgctggcgcg tcttatccga cctacgggga cgcatgtgta ggccggataa ggcatttacg
    37801 ccgcatccgg caagggtgtc caaatgcaac acgctttatc cgttctggac ttcacccgct
    37861 aaccaacgcg ccgcagcaat aaccccctgc cccagagaca aaccgccatc acccgccggt
    37921 aaactctgtg gaaagagcaa tgtgaaatca gcgagataat gcgccagacg tgcacgcagc
    37981 aaacggttat gaataacccc gccgctaaat accagcgtag tgataccacg catcgtggcc
    38041 tgctcacgca tcaacgcggc aaaaccctgc gccagcgcat catgaaacgc ccacgcgcgt
    38101 tgattaacca gtgcctgcca gttcagccac tgctgccaga aagtggcgag atccagttga
    38161 ttgtccaccc gcggcatcgt caccggatgc gtcactccgt cgcatgaggc tgcgagcgcc
    38221 tccagagcac aagccgcttc accttcataa cttaacgtgg ctggcgcaca gcccagtgcc
    38281 gccgccactg catcgaacaa ccgcccacac gatgatgcca gcggcgcgtt aattccacgc
    38341 tcaatggccc gcgccagtac gctccagttt tgctgttgca cacttgccgt ttcagggtaa
    38401 ttctgccact ccggcacaaa gcgcaggcac tgcgccagca ggtttcgcca cggctgcttc
    38461 gccgccaaat cgccacccgg aagcgccact gcaggcaagc cgcccaggtg ctcacattcg
    38521 cgatagttca cccgcaggca ctcgccgccc cacaaagcgc cattctcccc cataccgata
    38581 ccgtcgagcg tcaaagcaat gacatcaccg ccatccagcg gccactgatg ctctgccaga
    38641 cacgccgctg catgggcatg atgatgcagt accgtttgcg tcggcagatt catttcgctc
    38701 gcccactggc tggagacata gcccggatgc acatcatgca caatgtattg cggagtgaaa
    38761 tcgtagatgt tttgcatcag gcgtaacgct tcgcgccact gcatctggat gccatcgtca
    38821 cttaaatcgc ccagatgctg actcaacacc gcttgttcac cgcgcaccag gcagaaggta
    38881 tttttcagat ccgcgccaag acacagcaca ggcggaacat ttttaaagcc cggaggcaaa
    38941 accagcgcat ccggcacata cccccgcgaa cggcgcagca tttcgccgct ttcgcgcacc
    39001 actgaatcat ccatccgctg cacgatgtcg cggttatgta gcaagaatcc gtcggcaatg
    39061 ccctgcaaat ccgccagcgc ctgttcgttg ctgatagctg gtggtttacc gctcaggttg
    39121 ccggaggtca tcaccagcgg gcattgcagt tcctgtaaca gcaaatgctg gagcgggttc
    39181 gcaggcaaca tcaccccgac ttcgttaagg ccaggggcga tatcatcaca aagctcaggt
    39241 acgtattttt tatccaccag cacaatcggc gcagcgggcg tggtaagcaa ctggcgcgca
    39301 gcgtctggta aactttctgc cactggcaac atgactgcca gcggtttcgc cgggcgatgt
    39361 ttgcgctccc gcagtgtcgc caccgcgtta ctgttacgtg catcgcaggc aagatgaaat
    39421 ccgccaatcc ctttgatggc gacaatgttg cccattttta actgtgcgat agctgcctgt
    39481 aatgccgcct cttgttctgc atgttcacca tgacttaccc attcaagatg cgggccacac
    39541 tccgggcagg ccaccggctg ggcgtggaag cgacgatcga gcgggtcgcg gtactctttg
    39601 tcacaggccg gacatagcgg aaacgccgcc atcacggtaa acgggcggtc gtaaggcatc
    39661 gcgcgaataa tggtgaaacg cgggccgcag tgggtacagt tgataaacgg ataacgataa
    39721 cgccgctcgc ctggggtatt catttcggca aggcaagcag ggcaagtggc ggcatccggg
    39781 acaatttgcg tattcatggt gccgccggtg ctctggcgga tggtgaactc ggtgggcagt
    39841 tgtgaccaga taaacggctc acgctcgacg ctatcaatac gcgccagtgg cggacagtgc
    39901 tgatgcaatt gaacaagaaa cgtttccggg tcttccagca gccggacttc tacgccatcg
    39961 ccgtcattac atacatcgcc gtgaagattt aattgctgtg ccagctgcca gacaaacgga
    40021 cgaaaaccga cgccctgcac tttgccacga atacgcagtt ggacaccgca agatgtgttt
    40081 tttgccattg agttattccc gccatcatga attgcgtaac ccgccctgcc ggacacgaca
    40141 gcgtcgtatc cggcagtctc aggtcggcga tcccgcccgc tccgtattct acgaatattt
    40201 ccgggaattc ctttgatgcc agaacagttc tgtaagattt ttagaacatc agcgccgtac
    40261 ggcggcgttt ttctgcgctc agttgttcaa gtttattacg atcgacacaa atcagcgcat
    40321 gggtcgggca agccgccata cacgccgggc cgtcttcacg atggttgcac aggtcgcatt
    40381 tattggcttc ggctttgtca gcccgtacat tcagacccgc gccgctgttg cgaatcacag
    40441 gacgtaccac cacttccatc gcgccatacg ggcaagccac cacgcaggtt ttgcaaccaa
    40501 tgcaacgttc ctgcatcaca ggaacaaacc ctttatcacg gctgatagca ccattcgggc
    40561 agacgttagc gcacggtgca tcttcacact gacggcaaac tgtcgccgtg gaaatgttca
    40621 cacctttaat gacatggata cgcggtaaaa atgtttccgg ggtcagcgat gcacagtcct
    40681 gattttcctg atgagaaacc acgcacgcca cttcacaggt acggcaacca atacatttac
    40741 tcgcgtcagc aatgatgaaa cggttcatca aattctccag caatgacagt taatgcgccg
    40801 atacattcac aaatcatgcc agtttttaat ttgctgttat ttaaggaaat taatttctgt
    40861 aatgcaggaa aaaagatgtc atcgacacta gtgacgatga catgtgatga caatgtttat
    40921 cgcgaaaggg caatgagtga gtcgcggcgg atcagttttc cgctaaaggt tttcggcggt
    40981 gagaaatccc cgccatcgag cataaaaatc agccgtccaa taatttcctg aatcatctca
    41041 gtcaccggaa tttttacgct ggagagcgcc ggaacgatat agggggcaat tgcgatatca
    41101 tcgaatccga taactgacac ctgctctggc accgctacgc cgcgctcgtg caacgctttg
    41161 atcgcaccta tcgccatatc gtcgttactg gcaactaacg cgctaaattt agcccctcgt
    41221 tcgagcaacg tttctacccc ttcggccccg ctggcgggcg tccatttacc gttagcgata
    41281 agtttttcat tgagcgcaat accatgctgc gacagcgcgt ctttataccc ggcaagacgt
    41341 tcaatgctgg tgggggaatc catcgagccg gtaaggaaag caatctcctg atgcccggcg
    41401 tttatcaact ctgccacggc gttaaagctg gtctgtttat gatcgcacca gacgctatgg
    41461 ctgctattat tgcgcaggcg gcgattaagc accattatcg gctgactgtg cgcgtcaatg
    41521 atgtcatcaa tctcatccac gcttaaaaaa cgcgggtaaa tcataatggc gtcgcagcgc
    41581 aaatccagca gatactgaat cgcctggcgc tcttcttcgg cgctgtgttt accatctgcc
    41641 aatagcaact gtcgcccttt ctcttccgcc attcgcgcgg catgaaacag taattcacta
    41701 aaataaatgc cgtgataaag cgtgttggtc actaccagcc ccagcgtctg agtactcttc
    41761 gccgacagat tgcgggccag caaatttggg cggtaactgc tctcttctac cgcctgaaac
    41821 acgcgatctt tagtctcctg gctgacgtag ccattacctg aaagcacgcg ggaaacggtc
    41881 gcttttgaaa ccccggcgcg cttcgccact tccagcatcg tcgtcatcat tttcatccct
    41941 ttacacgcaa tcaacgcagt gtactgcacc gtttgccgat tgtccttgcg caatcggctc
    42001 cagcggataa gacgaggtcg ccgtgccggt tttgatgact tttttgataa aggtaaacat
    42061 ggcgattcct tatttcagcg gcgagttttt acgctcaatg ctgtaacgct cgagttcttt
    42121 gtacggcacc actttgctct tcttcttacg cacatcgact acggtcatgc ggtcggtaca
    42181 ggagtagcaa gggtccaggc taccgataat cagcggcgca tcggaaacgg tattgccacg
    42241 cagcatgtag cgcagggtcg gccagttcgc gtaggtcgcg gcacggcagc gccagcggta
    42301 cagcttctgg ttgtcgccgg tcatgctcca gtggatatcg tcgccgcgcg gcgcttcggc
    42361 aaagcccagc gcgaaacggt gcggaatgta ggtaaagcct tccaccatca gcggaccgcc
    42421 cggcaggtta tccagaccgt agtcgatcat attcagcgcg gtatagacct cgttgattcg
    42481 cactttcaga cgggaaataa cgtcgcagcc ctgctcgctg tggacttcca ttggcagcag
    42541 gccataaccg acaaacgggt gatcggcgcg agtatcgcgg gcgagaccgc tggcgcgaac
    42601 catcgggcca acgttactga aatcgcgggc gatttccggg tccagacgac caatgccgac
    42661 ggtgcgttgt tccatgttcg gcgtactcag cagcacatcc accagctcct gcacttcacg
    42721 acgcatttgt tgcgccagct ggcgggtctg gatcatgtca tctttcagca gatcgcgacg
    42781 aatcccgccg atcaggttca ggccgtaggt tttacgcgca ccggtaagga tctctgccat
    42841 tttcatggag gtttcacgca cgcggaagaa ctgcataaag ccggagtcga agccggtaaa
    42901 gtgacaggcg aggccgaggt tgagcagatg cgggtgcagg cgttccacct ccagcagaat
    42961 ggcgcggatc atctgcgcgc gttctggcac ctgaataccc atcgcgtttt ccaccgacgt
    43021 ggtgtaggcg gtgctgtggg caaagccgca gatcccgcac acacggtcag agaggaaggt
    43081 cacttcgtta taacccatgc gggtttccgc cagtttttcc ataccgcgat ggacgtagaa
    43141 cagacggtag tcggcgtcga taatgttttc gccatcgacg aacagacgga agtgacccgg
    43201 ttcgtcagaa gtgacgtgca gcggaccaat cggcacgacg ttgtttttct tgtcgcccag
    43261 ttcgttgatg aactcgtagg tttcagcatc ggtagtcggt gccggacgct gacgataatc
    43321 catgctgtct ttacgcagcg gataaagttc atccggccag tcatccggca gcaccagacg
    43381 acgttcatcc ggcagaccaa ccggaatcaa accgtacata tcgcgcactt cgcgctcgcc
    43441 ccacaccgcc gccggaacgc gcggcgtcac ggacggatac tccggtttgt tggcgtcaac
    43501 ttcgacgcga accgttaccc aacacttggt gcccttctcc atcgacagca cgtaataaac
    43561 ggcgtaatga ccattcagtt tgcgttcgtc gttaccaaac agcaccgaca gccagccacc
    43621 ctgcttgtag tagagaaact ccaccacttc cggcagatag ttcaccttta cggtgacagt
    43681 cagctgatct ttggtctgcc aggcgtggtc cagcacgacg cctagaaatg cctcattcag
    43741 cgcggcgaga taatgttgac ctaatttttc ttcagacatg ctcaaactct ctttaatcac
    43801 gccgccagca aggagacgaa cgctaaaaat gcaaagccaa acccagccca ggtaatgcgc
    43861 ggagtaatat caagacgcag acgggccatg ctgttttcga acagcgcgat aaccaggacg
    43921 ccgaccacca gtttgacgat ggcaatcacc agcgccagca gcagtccacc gacggtgaag
    43981 gtttccattt gtccccacgg aataaacacc ccgacgaaca tctgcaacac caccagctgt
    44041 ttcaggctga taccccattt catgacgcca aagccgctac cgctgtattc ggagagcgga
    44101 ccttcctgca actcctgctc ggcttccgcc aggtcgaacg gcagtttgcc catttcgata
    44161 aaagtggcga acgcacaggc gcagagcgcc agcaccagcg ggatgctttg gctcagcggc
    44221 cagtgataaa cggtgtcggt aatgttgctg atgttggtgg aaccggcaac ctgtgcggca
    44281 acccacagac caagcagcaa catcggttcg accagcacgc caagcatcgc ttcacggctc
    44341 gcaccgatag cggtaaacgg gctaccggta tccagaccgg aaatggcaaa gaagaaacgc
    44401 gcgatggcga agagatacag taaggtgatc aaatcgccca gttgcggcag cggagaaccg
    44461 acggtcacca ccggcagcgc agtagcgata gtcagcatga cgcccaccat cacatacggc
    44521 gtcaggcgga acatccagcc agaggcatcc gggccaacgc tctggcggcc cagcagtttg
    44581 ataatgtcgc gatactcctg caacacgccc ggcccgcgac ggttatgcag acgggcgcgc
    44641 gccacgcggg taataccgga gagcagcggc gcaacggcaa ataacaccag cgcctgaatt
    44701 aacggatata aaacactcat tctcaggctc ctcgtgaaac aataatcacc accagtaccg
    44761 ccagttcaac cagcgccatc cggcggaaca gcaacgcact accttcgcac tgccagcccg
    44821 gcactagcga caccggattc agccatttgc gcagtttcag cactggcgca aacgcctgtt
    44881 taaccggcat ggcaaaaccg tgagcggtaa tcaccattga tttttcatgg tcgtagccgc
    44941 acacccaggc cgcaccgcgg gaacgtgacg gcaggcggtc gcctttgcaa atcgccataa
    45001 tgatgaatgg cagcagcggg caggcaatca gcagcaatgt gatcattggt tgagaaacgg
    45061 tggtgttagc aggctccaga ggcagaggta cagcagcaga gagcatcggc agcagccacg
    45121 gcgcagcaac accgccaatt acgcagcaaa tcgccagtgc cactacgctt acgctcatca
    45181 ggagcggcgc acaggtggcg ttttcggctt ctttggtacg cggcgcgccg aggaacgtga
    45241 cgccatagac tttcgccata cacatcaccg ccagcgcacc ggtaattgcc agacccacag
    45301 cgagcagcgg ccccagcaga cgggcaacaa acgcgccgct attgctcagt ttgaagaagg
    45361 attgatagat aacccattcc ccggcaaacc cgttcagtgg cggcagcgca ccgctcacgc
    45421 caaccgcgcc agtcagtaca gtgaagcccg cggctgccgt atacagacta ccaaccgcgc
    45481 cgccaattcc ggctatccag ccacttagcg ctttttgaaa agagaaatgc cagaacagcg
    45541 gcggcgacaa accatgccac gccgctattg atcagggaaa ttgcgctcat ttagcctctc
    45601 cactttgagc ctgctgaaac aaggtgagat cgccaaagtc cgtgttaaag gtcagctcac
    45661 gcttacgttt gctgacgcgg gcgatatcgg tgttatccac cagatgcagg gctttagtcg
    45721 ggcacatccg cacacaggcc ggaccttgtt catcaaagct acaaaggtca catttgacgg
    45781 cgatcgcgcg aatacctggc acccagtcaa gcaatgtgct gacacgcgcc ggagcaggcg
    45841 gtgccggtgg cgctttcggg gtattggcgt ttgccggaat atccagcgga cggctgccgg
    45901 aaaattcaat tgcgccaaac gggcaggcga tgccgcacag tttgcagctt acgcacagac
    45961 tttcattcaa ctgcacggcc ccatcaacgc gggtgatggc gttaaccggg cagaccaccg
    46021 cgcagggtgc atcttcacag tggtgacaga gctgcggcgc agattctttt tcattgagca
    46081 tcactctcag gcgcggcatt gattgcaggc cgtgctggcg atgcgtctct gaacaggcgg
    46141 cctcacaggt gtggcagccg atacagagcg tggagtcggc aattacaaaa cgattcacca
    46201 ggcattcctc aggtgattgt catttttgac gaaaacatgc cgatgaaatg tcattttcga
    46261 cactcatcga cacgcccatc cccaaacagg cgtaacgcct gcaaaacggg caaagcctca
    46321 gctcatgctg ccgggctttg tccctttacc agttggctta aattcaccgg cacattgttt
    46381 tcaatggcgg tgtataagct gtcgtaaacg ccagcgattt tttcgagata gtgttccaga
    46441 acctgttcgc gatcgcggat gttgaccttg ccatcaatca tgccgtcaat gctcagttgc
    46501 gcctgcgcaa tccgctgttt gtcttcgcca tcttttgttt cgacgtaata ctcgatggtg
    46561 tggctgttaa agccatccgc cagggtgacg taaatacgaa aatgttcaaa aaggacgaaa
    46621 cagagttctt tgtccggcgc gcagctcatg gcgtgataca ggcgcgcttt cgataacgtg
    46681 atcccctgaa ccagcgaatt gcagtagatg tgccactggt cctgtaggcg acgatgccgc
    46741 tgtgcgatgt aatcggcttt ctcgcttatt tcccaaatag tcattgtcag gttactcgtt
    46801 taacagagat gccagcttta agcatttttt gtgccaactt ttaatttatt gttattaaag
    46861 agatttttaa gctaaagatg aatttcgtcg ccgtgtcgac gtgtcatttc gacatcatcg
    46921 acattattca ccgcagggat aatcaacact ggcgcaatta ttgcttgtag ctggcaatag
    46981 ttaatgggag gcgatatgca cgaaataacc ctctgccaac gggcactgga attgatcgaa
    47041 cagcaggccg caaaacacgg cgcaaaacgc gtaactgggg tctggctcaa aattggcgca
    47101 ttttcttgtg tcgaaaccag ctctcttgcc ttttgttttg atctggtttg ccgcggcagc
    47161 gtggcggaag gttgtaaact gcacctcgaa gaacaagagg ccgaatgctg gtgtgaaaca
    47221 tgccaacagt atgtgacgct actgacccag cgcgtccgcc gctgtccaca gtgtcatggt
    47281 gacatgctac agattgtggc agacgacggt ttacagattc ggcggataga aatagaccag
    47341 gagtgagcga tgtgtacaac atgcggttgc ggtgaaggca acctgtatat cgagggtgat
    47401 gaacataacc ctcattccgc gtttcgtagc gcgccatttg ccccggcggc acgcccgcag
    47461 atgaaaatca ccggcattaa agcgcctgaa tttaccccca gccagactga agaaggcgac
    47521 ctgcattacg gtcatggcga agcgggcact cacacaccgg gcatgagcca gcgtcggatg
    47581 ctggaagtcg aaattgacgt gctggacaaa aacaaccgtc tggctgaacg caaccgcgcg
    47641 cgctttgctg cccgcaagca actggtgctc aacctggttt ccagccctgg ttccggtaaa
    47701 accaccctgc tgacggaaac cttaatgcgc ctgaaagaca gcgttccgtg cgcggttatt
    47761 gaaggcgacc agcaaaccgt gaacgatgcc gcacgcattc gcgctaccgg tacaccagcg
    47821 attcaggtga acaccggtaa aggctgccat cttgacgcac agatgattgc cgacgccgca
    47881 ccgcgtctgc cactggacga taacggtatt ctgtttatcg aaaacgttgg caacctcgta
    47941 tgcccggcca gcttcgatct cggtgaaaaa cacaaagtag cggtgctttc cgttaccgaa
    48001 ggtgaagaca aaccgctgaa atatccgcat atgtttgccg ccgcctcgct gatgctgctc
    48061 aacaaagttg acctgttgcc gtatctcaac tttgacgttg agaagtgcat cgcctgcgcc
    48121 cgcgaagtca atccagaaat tgaaatcatc cttatttccg ccaccagcgg cgaagggatg
    48181 gaccagtggc tgaactggct ggagacacag cgatgtgcat aggcgttccc ggccagatcc
    48241 gcaccattga cggcaaccag gcgaaagtcg acgtctgcgg cattcagcgc gatgtcgatt
    48301 taacgttagt cggcagctgc gatgaaaacg gtcagccgcg cgtgggccag tgggtactgg
    48361 ttcacgttgg ctttgccatg agcgtaatta atgaagccga agcacgcgac actctcgacg
    48421 ccttacaaaa catgtttgac gttgagccgg atgtcggcgc gctgttgtat ggcgaggaaa
    48481 aataatgcgt tttgttgatg aatatcgcgc gccggaacag gtgatgcagt taattgagca
    48541 tctgcgcgaa cgtgcttcac atctctctta caccgccgaa cgccctctgc ggattatgga
    48601 agtgtgtggc ggtcataccc acgccatttt taaattcggc ctcgaccagt tactgcctga
    48661 aaacgttgag tttatccacg gtccgggttg cccggtgtgc gtactgccga tgggtagaat
    48721 cgacacctgc gtggagattg ccagccatcc ggaagtcatc ttctgtacct ttggcgacgc
    48781 catgcgcgta ccagggaaac agggatcgct gttgcaggca aaagcacgcg gtgccgatgt
    48841 gcgcatcgtt tactcgccga tggatgcgtt gaaactggcg caggagaatc caacccgcaa
    48901 agtggtgttc ttcggcttag gttttgaaac caccatgccg accaccgcca tcactctgca
    48961 acaggcgaaa gcgcgtgatg tgcagaattt ttacttcttc tgccagcaca ttacgcttat
    49021 cccgacgctg cgcagtttgc tggaacagcc ggataacggt atcgacgcgt tcctcgcgcc
    49081 gggccacgtt agtatggtta tcggcactga tgcctataat tttatcgcca gcgattttca
    49141 tcgtccgctg gtggtcgctg gtttcgaacc ccttgatcta ctgcaaggcg tggtcatgct
    49201 ggtggagcag aaaatagcgg cccacagcaa ggtagagaat cagtatcgtc gggtggtacc
    49261 ggatgccggt aacctgctgg cgcaacaggc gattgccgat gtgttctgtg tcaacggcga
    49321 cagcgaatgg cgcggcttag gcgtgattga atcttctggc gtgcacctga cgccggatta
    49381 tcaacgattc gatgccgaag cacatttccg cccggcaccg cagcaggtct gcgatgaccc
    49441 gcgcgcgcgt tgtggtgagg tattaacggg caaatgtaag ccgcatcaat gcccgctgtt
    49501 tggtaacacc tgtaatcctc aaaccgcatt tggtgcactg atggtctcct ccgaaggagc
    49561 gtgcgccgcg tggtatcagt atcgtcagca ggagagtgaa gcgtgaataa tatccaactc
    49621 gcccacggca gcggcggcca ggcgatgcag caattaatca acagcctgtt tatggaagcc
    49681 tttgccaacc cgtggctggc agagcaggaa gatcaggcac gtcttgatct ggcgcagctg
    49741 gtagcggaag gcgaccgtct ggcgttctcc accgacagtt acgtcattga cccgctgttc
    49801 tttcctggcg gtaatatcgg caagctggcg atttgcggca ccgcgaatga cgttgcgggc
    49861 agtggcgcta ttccgcgcta tctctcctgt ggctttatcc tcgaagaagg attgccgatg
    49921 gagacactga aagccgtagt gaccagcatg gcagaaaccg cccgcacggc aggcattgcc
    49981 atcgttactg gcgatactaa agtggtgcag cgcggcgcgg cagataaact gtttatcaac
    50041 accgctggca tgggcgcaat tccggcgaat attcactggg gcgcacagac gctaaccgca
    50101 ggcgatgtat tgctggttag cggtacactc ggcgaccacg gggcgactat ccttaacctg
    50161 cgtgagcagc tggggctgga tggcgaactg gtcagcgact gcgcggtgct gacgccgctt
    50221 attcagacgc tgcgtgacat tcccggcgtg aaagcgctgc gtgatgccac ccgtggtggt
    50281 gtaaacgcgg tggttcatga gttcgcggca gcctgcggtt gtggtattga aatttctgaa
    50341 tcagcgctgc cggttaaacc tgccgtgcgc ggcgtttgcg aattgctggg actggacgcc
    50401 ctgaactttg ccaacgaagg caaactggtg atcgccgttg aacgcaacgc ggcagagcaa
    50461 gtgctggcag cgttacattc ccatccactg gggaaagacg cggcgctgat tggtgaagtg
    50521 gtggaacgta aaggtgttcg tcttgccggt ctgtatggcg tgaaacgaac cctcgattta
    50581 ccacacgccg aaccgcttcc gcgtatatgc taataaaatt ctaaatctcc tatagttagt
    50641 caatgacctt ttgcaccgct ttgcggtgct ttcctggaag aacaaaatgt catatacacc
    50701 gatgagtgat ctcggacagc aagggttgtt cgacatcact cggacactat tgcagcagcc
    50761 cgatctggcc tcgctgtgtg aggctctttc gcaactggta aagcgttctg cgctcgccga
    50821 caacgcggct attgtgttgt ggcaagcgca gactcaacgt gcgtcttatt acgcgtcgcg
    50881 tgaaaaagac acccccatta aatatgaaga cgaaactgtt ctggcacacg gtccggtacg
    50941 cagcattttg tcgcgccctg atacgctgca ttgcagttac gaagaatttt gtgaaacctg
    51001 gccgcagctg gccgcaggtg ggctataccc caaatttggt cactattgcc tgatgccact
    51061 ggcggcggga gggcatattt ttggtggctg tgaatttatt cgttatgacg agcgtccctg
    51121 gagcgaaaaa gagttcaatc gtctgcaaac atttacgcag atcgtttctg tcgtcaccga
    51181 acaaatccag agccgcgtcg tcaacaatgt cgactatgag ttgttatgcc gggagcgcga
    51241 taacttccgc atcctggttg ccattaccaa cgcggtgctt tcccgcctgg atatggacga
    51301 actggtcagc gaagtcgcca aagaaatcca ttactatttc gacattgacg atatcagcat
    51361 tgtcttacgc agccaccgta aaaacaaact caacatctac tccactcact atcttgataa
    51421 acaacatccc gcccacgaac agagcgaagt cgatgaagcc ggaaccctca ccgaacgcgt
    51481 gttcaaaagt aaagagatgc tgctgatcaa tctctacgag cgggacgatt tagcccccta
    51541 tgaacgcatg ttgttcgaca cctggggcaa ccagattcaa accttgtgcc tgttaccgct
    51601 gatgtctggc gacaccatgc tgggcgtgct gaaactggcg caatgcgaag agaaagtgtt
    51661 taccactacc aatctgaatt tgctgcgcca gattgccgaa cgtgtggcaa tcgctgtcga
    51721 taacgccctc gcctatcagg aaatccatcg tctgaaagaa cggctggttg atgaaaacct
    51781 cgccctgacc gagcagctca acaatgttga tagtgaattt ggcgagatta ttggccgcag
    51841 cgaagccatg tacagcgtgc ttaaacaagt tgaaatggtg gcgcaaagtg acagtaccgt
    51901 gctgatcctc ggtgaaactg gctcgggtaa agagctgatt gcccgtgcga tccataatct
    51961 cagtgggcgt aataatcgcc gcatggtcaa aatgaactgc gcggcgatgc ctgccggatt
    52021 gctggaaagc gatctgtttg gtcatgagcg tggggctttt accggtgcca gcgcccagcg
    52081 cattggtcgt tttgaactgg cggataaaag ctctctgttc ctcgacgaag tgggcgatat
    52141 gccactggag ttacagccga agttgctgcg tgtattgcag gaacaggagt ttgaacgtct
    52201 cggcagcaac aaaatcattc agacggacgt acgtttaatt gccgcgacta accgcgatct
    52261 gaaaaaaatg gtcgccgacc gagagttccg tagcgatctc tattaccgcc tgaacgtatt
    52321 cccgattcac ctgccgccac tacgcgagcg tccggaagat attccgctgc tggcgaaagc
    52381 ctttaccttc aaaattgccc gtcgtctggg gcgcaatatc gacagcattc ctgccgagac
    52441 gctgcgcacc ttgagcaaca tggagtggcc gggtaacgta cgcgaactgg aaaacgtcat
    52501 tgagcgcgca gtattgctaa cacgcggcaa cgtgctgcag ctgtcattgc cagatattgc
    52561 tttaccggag cctgaaacgc cgcctgccgc aacggttgtc gcccaggagg gcgaagatga
    52621 atatcagttg attgtgcgcg tgctgaaaga aaccaacagc gtggttgccg ggcctaaagg
    52681 cgctgcgcaa cgtctggggc tgaaacgcac gaccctgctg tcacggatga agcggctggg
    52741 aattgataaa tcggcattga tttaactgca aattgccgga cagatctgcc tgtccggcat
    52801 actattcacg aggttttttc ggacgatatt tttccggcag ttctggcacc ggacgcttgt
    52861 catcgatgag atgacgcacg gttaagatcg gatgacgcca cagcattctc ggcccggccc
    52921 aacgcataat ctgtttcatc tcttcacgct ttgcaggctg gtaacagtgc accggacact
    52981 gcttacaggc tggtttctct tcgccgaaca cacatttatc cagccgcttt tgcgcgtaaa
    53041 caaacaacgc ctcgtaatgc tccggctccg ctgacgcctg cgggcatttc gcttgataaa
    53101 gatcgatcat ttttttaatc gtcagttttt cacgagagat acgcttgccg gacatgctgc
    53161 ctccacctca ttaagatgca tttatattac atcttaat
//
LOCUS       DAEEUE010000018        52078 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.018, whole genome shotgun sequence.
ACCESSION   DAEEUE010000018 DAEEUE010000000
VERSION     DAEEUE010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52078
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.018"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(262..2541)
                     /gene="maeB"
                     /locus_tag="KLK84_001131"
     CDS             complement(262..2541)
                     /gene="maeB"
                     /locus_tag="KLK84_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HBE2008541.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2830..3780
                     /gene="tal"
                     /locus_tag="KLK84_001132"
     CDS             2830..3780
                     /gene="tal"
                     /locus_tag="KLK84_001132"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBE2008542.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3800..5803
                     /gene="tkt"
                     /locus_tag="KLK84_001133"
     CDS             3800..5803
                     /gene="tkt"
                     /locus_tag="KLK84_001133"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBE2008543.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5906..6949)
                     /locus_tag="KLK84_001134"
     CDS             complement(5906..6949)
                     /locus_tag="KLK84_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HBE2008544.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(7075..7650)
                     /gene="nudK"
                     /locus_tag="KLK84_001135"
     CDS             complement(7075..7650)
                     /gene="nudK"
                     /locus_tag="KLK84_001135"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HBE2008545.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(7718..9697)
                     /gene="aegA"
                     /locus_tag="KLK84_001136"
     CDS             complement(7718..9697)
                     /gene="aegA"
                     /locus_tag="KLK84_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HBE2008546.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVLIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9903..11620
                     /gene="narQ"
                     /locus_tag="KLK84_001137"
                     /pseudo
     CDS             9903..11620
                     /gene="narQ"
                     /locus_tag="KLK84_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            11822..14935
                     /gene="acrD"
                     /locus_tag="KLK84_001138"
     CDS             11822..14935
                     /gene="acrD"
                     /locus_tag="KLK84_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HBE2008547.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(15034..15093)
                     /gene="ypfM"
                     /locus_tag="KLK84_001139"
     CDS             complement(15034..15093)
                     /gene="ypfM"
                     /locus_tag="KLK84_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HBE2008548.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            15474..15830
                     /locus_tag="KLK84_001140"
     CDS             15474..15830
                     /locus_tag="KLK84_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HBE2008549.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            15834..16961
                     /gene="dapE"
                     /locus_tag="KLK84_001141"
     CDS             15834..16961
                     /gene="dapE"
                     /locus_tag="KLK84_001141"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HBE2008550.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            16989..17189
                     /locus_tag="KLK84_001142"
     CDS             16989..17189
                     /locus_tag="KLK84_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HBE2008551.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(17299..17997)
                     /gene="ypfH"
                     /locus_tag="KLK84_001143"
     CDS             complement(17299..17997)
                     /gene="ypfH"
                     /locus_tag="KLK84_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBE2008552.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(18071..20086)
                     /gene="tmcA"
                     /locus_tag="KLK84_001144"
     CDS             complement(18071..20086)
                     /gene="tmcA"
                     /locus_tag="KLK84_001144"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HBE2008553.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(20101..20964)
                     /locus_tag="KLK84_001145"
     CDS             complement(20101..20964)
                     /locus_tag="KLK84_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HBE2008554.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(21132..21845)
                     /gene="purC"
                     /locus_tag="KLK84_001146"
     CDS             complement(21132..21845)
                     /gene="purC"
                     /locus_tag="KLK84_001146"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBE2008555.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(22058..23092)
                     /gene="bamC"
                     /locus_tag="KLK84_001147"
     CDS             complement(22058..23092)
                     /gene="bamC"
                     /locus_tag="KLK84_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HBE2008556.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDVGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(23109..23987)
                     /gene="dapA"
                     /locus_tag="KLK84_001148"
     CDS             complement(23109..23987)
                     /gene="dapA"
                     /locus_tag="KLK84_001148"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBE2008557.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            24133..24705
                     /locus_tag="KLK84_001149"
     CDS             24133..24705
                     /locus_tag="KLK84_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HBE2008558.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            24705..25175
                     /gene="bcp"
                     /locus_tag="KLK84_001150"
     CDS             24705..25175
                     /gene="bcp"
                     /locus_tag="KLK84_001150"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBE2008559.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            25428..26045
                     /gene="hyfA"
                     /locus_tag="KLK84_001151"
     CDS             25428..26045
                     /gene="hyfA"
                     /locus_tag="KLK84_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HBE2008560.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            26045..28062
                     /gene="hyfB"
                     /locus_tag="KLK84_001152"
                     /pseudo
     CDS             26045..28062
                     /gene="hyfB"
                     /locus_tag="KLK84_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            28073..29020
                     /gene="hyfC"
                     /locus_tag="KLK84_001153"
     CDS             28073..29020
                     /gene="hyfC"
                     /locus_tag="KLK84_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HBE2008561.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            29037..30476
                     /gene="hyfD"
                     /locus_tag="KLK84_001154"
     CDS             29037..30476
                     /gene="hyfD"
                     /locus_tag="KLK84_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HBE2008562.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVVSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            30488..31138
                     /gene="hyfE"
                     /locus_tag="KLK84_001155"
     CDS             30488..31138
                     /gene="hyfE"
                     /locus_tag="KLK84_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HBE2008563.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            31143..32729
                     /gene="hyfF"
                     /locus_tag="KLK84_001156"
     CDS             31143..32729
                     /gene="hyfF"
                     /locus_tag="KLK84_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HBE2008564.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            32719..34433
                     /gene="hyfG"
                     /locus_tag="KLK84_001157"
                     /pseudo
     CDS             32719..34433
                     /gene="hyfG"
                     /locus_tag="KLK84_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            34443..34988
                     /gene="hyfH"
                     /locus_tag="KLK84_001158"
     CDS             34443..34988
                     /gene="hyfH"
                     /locus_tag="KLK84_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HBE2008565.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            34985..35743
                     /gene="hyfI"
                     /locus_tag="KLK84_001159"
     CDS             34985..35743
                     /gene="hyfI"
                     /locus_tag="KLK84_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HBE2008566.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGTVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            35736..36149
                     /gene="hyfJ"
                     /locus_tag="KLK84_001160"
     CDS             35736..36149
                     /gene="hyfJ"
                     /locus_tag="KLK84_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HBE2008567.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            36179..38191
                     /gene="hyfR"
                     /locus_tag="KLK84_001161"
     CDS             36179..38191
                     /gene="hyfR"
                     /locus_tag="KLK84_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HBE2008568.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWQARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLSSVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            38213..39061
                     /locus_tag="KLK84_001162"
                     /pseudo
     CDS             38213..39061
                     /locus_tag="KLK84_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(39099..40160)
                     /locus_tag="KLK84_001163"
     CDS             complement(39099..40160)
                     /locus_tag="KLK84_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBE2008569.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            40373..41836
                     /gene="bepA"
                     /locus_tag="KLK84_001164"
     CDS             40373..41836
                     /gene="bepA"
                     /locus_tag="KLK84_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HBE2008570.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            41857..42216
                     /gene="arsC"
                     /locus_tag="KLK84_001165"
     CDS             41857..42216
                     /gene="arsC"
                     /locus_tag="KLK84_001165"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HBE2008571.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(42349..43095)
                     /gene="hda"
                     /locus_tag="KLK84_001166"
     CDS             complement(42349..43095)
                     /gene="hda"
                     /locus_tag="KLK84_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HBE2008572.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(43145..44434)
                     /gene="uraA"
                     /locus_tag="KLK84_001167"
     CDS             complement(43145..44434)
                     /gene="uraA"
                     /locus_tag="KLK84_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBE2008573.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(44520..45146)
                     /gene="upp"
                     /locus_tag="KLK84_001168"
     CDS             complement(44520..45146)
                     /gene="upp"
                     /locus_tag="KLK84_001168"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBE2008574.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            45471..46508
                     /gene="purM"
                     /locus_tag="KLK84_001169"
     CDS             45471..46508
                     /gene="purM"
                     /locus_tag="KLK84_001169"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBE2008575.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            46508..47146
                     /gene="purN"
                     /locus_tag="KLK84_001170"
     CDS             46508..47146
                     /gene="purN"
                     /locus_tag="KLK84_001170"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBE2008576.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVQAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            47318..49384
                     /gene="ppk1"
                     /locus_tag="KLK84_001171"
     CDS             47318..49384
                     /gene="ppk1"
                     /locus_tag="KLK84_001171"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HBE2008577.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            49389..50930
                     /gene="ppx"
                     /locus_tag="KLK84_001172"
     CDS             49389..50930
                     /gene="ppx"
                     /locus_tag="KLK84_001172"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HBE2008578.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(50969..>52078)
                     /gene="pdeF"
                     /locus_tag="KLK84_001173"
     CDS             complement(50969..>52078)
                     /gene="pdeF"
                     /locus_tag="KLK84_001173"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HBE2008579.1"
                     /translation="YKQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALD
                     SHLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQR
                     CGAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDEL
                     IGPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFP
                     VEVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYAS
                     YARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIRE
                     AVLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        12160 a        13738 c        13519 g        12661 t
ORIGIN      
        1 cgtgacaatt tttcgtcaga gtaaataaca aaattcggca agcgcgttta aaaggtgagg
       61 tagatcaata agaatggaga gatatttact gttatgaata aactttcata tgcgacgcat
      121 gaatataaat aaaataatat cgagatattt tatatactga tttttttatt attcgcaaac
      181 tcagtaataa gctttgctaa tatatttcag gaaaaccgct taccctgaat attcagggta
      241 agcgtgagag ttaagaatga attacagcgg ttgggtttgc gcttcaacca cggccagcgc
      301 caccatattg acgatacgac gcaccgatgc gatcggcgtt aacacgtgaa ccggtttcgc
      361 cacgcccatc agcaccggac cgacagtcac accttccgag ctggaaacgc gcagtaagtt
      421 gtaactaatg cgggcagctt ccatgttcgg catcaccaga atattggcag aacctttcaa
      481 agggctgtcc ggcatacggt cgttgcgaat cgcttccacc agcgctgcat cgccgtgcat
      541 ttcaccatca atcatcagat ctggcgcacg atccctgacc agttccagcg cctgacgcat
      601 tttgctcgac gacgggcagt cagaagaacc aaagttggag tgcgacaaca gagcaacgcg
      661 cggctcaata ccaaaacgac ggacagtttc tgccgccatc aaggtgatct ccgccagctc
      721 ttctgcgtct ggttcgtcat tgacgtaggt atcggcaata aaggtgttac cactcggcag
      781 cagcagcgcg ttcattgcac ctgcggtgtg aacgccatcg cgataaccaa agacattttt
      841 caccacgcta aaatgttcat gataatcgcc caccgtaccg cagatcattg catcggcttc
      901 cccacgctga accatgatcg cgccgatcac tgtcgggtta ctgatcagcg cacgctgcgc
      961 ctgttcctga gtgacaccgc gacgcttcat gatctggaag tattcggtcc agtactcttt
     1021 aaagcgcgga tcggattcgt tattgacgat ttcaaaatca acgcccgctt tgatctgcaa
     1081 gcccagtttc tgaatgcgca tttcgatcac gttcggacga ccgataagga tcggtttcgc
     1141 cagtcccagc gttaccagtt cctgagtcgc atgcagaacg cgcgcctctt ccccttccgg
     1201 cagaactacg cgcttcggcg ctttgcgagc ctgggagaaa atcggcttca taaacaggtt
     1261 ggttttgtaa acgaactcag tcagcttgtc gatgtagacg tcgaaatcag caatcggacg
     1321 cgtcgccacg cccgactcca tcgccgcttt tgcgaccgca ggagcgatct taacgatcaa
     1381 gcgcggatca aacggttttg gaatgatgta ttccggacca aagctcagat cctgaacgcc
     1441 atacgctgaa gccaccactt cactctgttc tgcatgggcc agttctgcaa tcgcgcgtac
     1501 cgccgccagt ttcatctctt cgttgatggc ggttgcgcca acgtccagcg cgccacggaa
     1561 gatgaacggg aagcaaagaa cgttgttcac ctggttcggg tagtcggaac ggccggtaca
     1621 gatgatggca tccggacgca cttctttcgc cagcggcggc agaatttccg gttccgggtt
     1681 cgccagcgcc aggatcattg gtgcccgagc cattttcttc accatttcct gggtcagcac
     1741 tttcgggccg gaacagccca ggaaaatatc cgcaccttca atcacatcat cgagggtacg
     1801 tttgccgtca tccaccaccg catatgcggc tttggtttcc gccatatttg gctcacggcc
     1861 ctgatagata acgccttttg aatcgcaaac cacgatgtta tgtttttgca gccccagcgc
     1921 taccagcagg ttcatacagg cgattgctgc ggcacccgcg ccggaaacca ccatccgcac
     1981 gtcggagatg tttttctcca ccacgcgcaa gccgttgagg atggcggcag tgctgatgat
     2041 tgccgtgccg tgctgatcgt cgtggaatac cggaatattc atccgctcgc gcagtttctg
     2101 ttcaatatag aaacattctg gcgctttaat gtcttcgagg ttgatgccgc cgaaggtcgg
     2161 ttcgagcgcg gcgacgactt caataaattt gtccgggtcg agttcatcaa cttcaatgtc
     2221 aaatacatca atcccagcga atttcttaaa tagaacgccc ttgccttcca tcaccggttt
     2281 gcctgccagc gcgccaatgt tgcctaaccc cagcaccgcc gtaccgttag agatcaccgc
     2341 aaccaggtta ccgcgcgcgg tatatttgta ggcttttagc gggtcttttt cgatttcaag
     2401 acaaggtgcg gcaacgcctg gtgagtaggc cagcgccaga tcgcgctgcg ttgccagagg
     2461 cttggttgga gaaacctgga ttttccctgg aactggaaat tcatggaaat caagtgcact
     2521 ttgttttaac tggtcatcca tttggttgtt cctttcacgt aacgttcaca aataaagtgt
     2581 gtgggcaaca gcccctgccc acaacgtggc gcacattatt accctgccgg agtctacaga
     2641 ctttgagcaa gtccaaactc tcaccattaa tataatgttt tggtaataat cctataacac
     2701 tgatgttacc tgcttaatcc agcaatacca tgcctgtctg ctatgctttt ttgatgcgtt
     2761 tagcgaaatt tctcagaagt gtgaattaac gcactcatct aacactttac ttttcaagga
     2821 gtatttccta tgaacgagtt agacggcatc aaacagttca ccactgtcgt ggcagacagc
     2881 tgcgatattg agtccattcg ccattatcat ccccaggatg ccaccactaa tccttcgctg
     2941 ttactcaagg ctgccggatt atcacaatat gagcatttaa tagacgatgc tatcgcctgg
     3001 ggtaaaaaaa atggcaagac ccaggaacaa caggtggtcg cagcgtgtga caaactggcg
     3061 gtcaatttcg gtgctgaaat tctcaaaatc gtacccggtt gcgtgtcaac agaagttgat
     3121 gcacgcctct cttttgataa agaaaagagt attgagaagg cgcgccatct ggtggacttg
     3181 tatcagcaac aaggcgttga gaaatcacgc attttgatca aactggcttc gacctgggaa
     3241 ggaattcgcg cggccgaaga gctggaaaaa gaaggtatta actgcaacct gacgctgctg
     3301 ttttctttta cccaggcgcg ggcctgtgcg gaagcaggcg tttttctgat ttcgccgttt
     3361 gtagggcgta tttatgactg gtatcaggca cgcaagccga tggacccgta tgtggtggaa
     3421 gaagatccgg gcgttaaatc ggtgcgcaat atctacgact actataagca acaccactat
     3481 gaaaccattg tgatgggcgc gagcttccgt cgcaccgaac aaatcctcgc cttaaccggc
     3541 tgcgatcgac tgactatcgc accgaatttg ctgaaggagc tgcaggaaaa agtttctcca
     3601 gtggtacgta aattaatccc gccttctcag acgttcccac gcccagcacc catgagcgaa
     3661 gcggagttcc gttgggagca caatcaggat gcgatggcgg tagaaaaact gtctgaaggc
     3721 attcgtctgt tcgctgttga tcaacgcaaa ctggaagatc ttcttgccgc caaactataa
     3781 accagccacg gagtgttata tgtcccgaaa agaccttgcc aatgcgattc gcgcactcag
     3841 tatggatgcg gtacaaaaag ccaattctgg tcatcccggc gcgccgatgg gcatggctga
     3901 tattgccgaa gtgctgtgga acgattttct taaacataat cctaccgacc caacctggta
     3961 tgatcgcgac cgctttattc tttccaacgg tcacgcgtcg atgctgctct acagtttgct
     4021 acatctgacc ggttacgacc tgccgctgga agaactgaag aacttccgtc agttgcattc
     4081 gaaaacccca ggccacccgg agattggcta tacgccaggc gttgaaacca ccaccggccc
     4141 gcttggacaa ggtttggcga acgccgtcgg gctggcgata gcagagcgta cactggcggc
     4201 gcagtttaac cagccagacc atgagatcgt cgatcacttc acctatgtgt ttatgggcga
     4261 cggctgcctg atggaaggta tttcccacga agtctgttcg ctggcaggca cgctgggact
     4321 aggcaagctg attggttttt acgatcacaa cggtatttcc atcgacggtg aaacagaagg
     4381 ctggtttacc gacgatacgg caaaacgttt tgaagcctat cactggcatg tgatccatga
     4441 aatcgacggt cacgatccgc aggcggtgaa ggaagcgatc cttgaagcgc aaagcgtgaa
     4501 agataagccg tcgctgatta tctgccgtac ggtgattggc tttggttcgc cgaataaagc
     4561 aggtaaggaa gaggcgcacg gcgcaccact gggggaagaa gaagtggcgc tggcacggca
     4621 aaaactgggc tggcaccatc cgccatttga gatccctaaa gagatttatc acgcctggga
     4681 tgcccgtgaa aaaggcgaaa aagcgcagca gagctggaat gagaagtttg ccgcctataa
     4741 aaaggctcat ccgcaactgg cagaagagtt tccccgacgg atgagcggtg gtttaccgaa
     4801 ggactgggag aaaacgactc agaaatatat caatgagtta caggcaaatc cggcgaaaat
     4861 cgctacccgt aaggcttcgc aaaatacgct taacgcttac gggccgatgc tgcctgagtt
     4921 gctcggcggt tcggcggatc tggctcccag caacctgacc atctggaaag gttctgtttc
     4981 gctgaaggaa gatccggcgg gcaactacat tcactacggg gtgcgtgaat ttggcatgac
     5041 cgctatcgcc aacggcatcg cgcaccacgg cggctttgtg ccgtataccg cgacgttcct
     5101 gatgtttgtt gaatacgccc gtaacgccgc gcggatggcg gcactgatga aagcgcggca
     5161 gattatggtt tatacccacg actcaattgg cctgggcgaa gatggtccga cgcaccaggc
     5221 tgttgagcaa ctggccagcc tgcgcttaac gccaaatttc agcacctggc gaccgtgcga
     5281 tcagatggaa gcggcggtgg gctggaagct ggcggttgag cgccacaacg gaccgacggc
     5341 actgatcctc tcaaggcaga atctggccca ggtggaacgt acgccggatc aggttaaaga
     5401 gattgctcgt ggcggttatg tgctgaaaga cagcggcggt aagccagata ttattctgat
     5461 tgccaccggt tcagagatgg aaattaccct gcaagcggca gagaaattag caggagaagg
     5521 tcgcaatgta cgcgtagttt ccctgccctc gaccgatatt ttcgacgccc aggatgagga
     5581 atatcgggag tcggtgttgc cttctaacgt tgcggctcgc gtggcggtgg aagcaggtat
     5641 tgccgattac tggtacaagt atgttggtct gaaaggggca attgtcggga tgacgggtta
     5701 cggggaatct gctccggcgg ataagctgtt cccgttcttt ggctttaccg ccgagaatat
     5761 tgtggcaaaa gcgcataagg tgctgggagt gaaaggtgcc tgatggtgat cgccggatgc
     5821 tgtatgttcc cctcacccta accctctccc cagaagggcg aggggacggt ccgtgcgctt
     5881 tttagggccg catctgcgat tttgtttaac gggtgatcca caacgtgggc ctggcgtctg
     5941 gcccatgcca gttatcgcag gtaggttctg cggcgtaacg caccaggcga aaacgctgac
     6001 cgtcaaagcg ccagcgcgcc tgaatgccac aatcgcttaa tccgcgccct ttcgctaagg
     6061 tcaccagttc acgcgatttc tcatcaaatg ttgcgttcat cagttccagt tcattcgtct
     6121 cctggccgtt gttgaacggc aaacgcaacc gaaccgggcg cgaggctagc ggctttttac
     6181 gcgacacaat ccatgccaaa tcaatggtgt tataggcccc tgcctcacag ctaatcatca
     6241 tcagtgcttt atcatcggtc agcgcagtga cattcacctc acgacgtaac ggatcaagcg
     6301 agcagcgcag accattcatc cgccagtttc cataatccag caaatcgtta cgttcttcga
     6361 gtgagagtgg cgtcggcgtt gggttaacca ccgcgacctc tttcagcgca ggcgcgggcg
     6421 gtacgctgag aggcggttcg tcccctttct tgatccacgc ggtttcactg ccaacgcgct
     6481 tttgctgagc atcaataaac aacaacgccg cttttaagcc gctcagagaa atggtctgat
     6541 tgccgtcgcg taaggtgatc gccttccctt cctgaattat ctgcaaaaac gcggtgatgg
     6601 ttgccgtatc atcggttact aataaccatg gtgaaatccg ccacttgtca ccacttaatg
     6661 ccaacggctc accatctaat agcagccgtg gcgctatctc cccttcagac gcatccggcg
     6721 acttcaaccc accgcgctca atacgtaaaa cggcatcggt atgcgcccca gcgctgcggc
     6781 tcagggtcat caccagtcca ttatgatcgc ccgtattgcg cgccacacaa aaattttgat
     6841 tattacaggt gacctgccag tcggaaaacg cccgttgcgc tggtgccgcc cacaccaaag
     6901 acgtcggcaa caaagcgaaa aaaaagagaa ggaaaatgcg atagcgcatg gacggtacga
     6961 ccccagaagt tcaaacaaac agcacaagtc gtatcttcgt tgcgcaggta gcgcggctca
     7021 atcggatttg tcggattgag ccaataataa acaacgaaat attattgcta tttttcagtc
     7081 cattaaatgt gacatttgca aatagttaag caataacacc gtcttaccgt cacgtatctc
     7141 gccggttttg atcatctcca gcgcctggct gaacggcagc tcgagcactt caatatcttc
     7201 atcttcaaca ccgccaccgg cgttagcgcg ctgattgtca ctgtattcgg cgataaaaaa
     7261 gtggattagc tcagtcacac cgccaggcga catatacagt tcaaataatt tgcgcacttc
     7321 gcccacttca tagcccgtct cttcaatcgc ttctttacga atgcacactt ccggttcgtc
     7381 gttatccagc agcccggcgc aggtttcaat cagctgcccg ctttcattgc cattaaccca
     7441 ggtagcgaca cggaactgac gaatcagaac cacgctcttt ttcttcgcgt tgtacaggag
     7501 gatcgtcgca ccattaccgc gatcgtaaac ttcacgttta tggcggataa cttcgccatc
     7561 tttgcgcgtg agatcgtaag taatgttgtg cagggtgaaa tagttatcgg agagaatttt
     7621 gtctttaatg agggtgattt gttgcgtcat accgactcca cagcgcgaaa tgaacaatta
     7681 tattacgctg tgaagtcggg tttgtctgcg caggctatca gtgagatttg actgatttta
     7741 cccccagcca gtcaataatc ccctgtgccg catgacgtcc ttctgccatt gcggtaacca
     7801 ccagatccgc accacgcacg gcgtcgccac cggcgaagat tttcggattg gtggtctggt
     7861 aacggtactg gctttccaca tccgcgatga tgcggcccca tttgtctacc gttacaccgt
     7921 gcgactccag ccacggcatc ccgtgtggat tgaagccaaa cgccataatc accgcatcgg
     7981 ctggcatgac aaattcactg ccctcaatca gtacaggtcg ccgacgcccc tgggcatccg
     8041 gctcgccaag acgcgtgcgc aggaaacgaa ttccgcaaac gtgaccttgc tcattcagct
     8101 caagcgccac cggctggacg ttaaattcga agttggcccc ctcttcgcgg gcgttcttca
     8161 cctctttctt cgagcctggc atgttagctt catcacgacg ataagcgcag gtgacgttac
     8221 tcgcgccgtg gcgcagcgca gtacgcacac agtccatcgc ggtgtcgccg ccgcccagta
     8281 ccacgacgtt aagtccggcg gtattgataa acggctcttc cggtagctct tcgagcccca
     8341 tcacctgttt agtgttggca atgaggaacg gcagcgcgtc ataaacgccc ggcgcatctt
     8401 cattgggtaa acccgctttc atggaacggt aagtgcctac gccaacgaag accgcgtcgt
     8461 attgttccaa aagcaaatcc aaagagacat ctttacccac ttcacaattg agttcgaagt
     8521 gaatccccat cgcgctgaag atttcccgac ggcgtgccag cagggattta tccagtttga
     8581 aagaaggaat gccgaaagtg agcaagccac cgatttctgg atggcgatcg tacaccgtca
     8641 ccgccacgcc attgcgggtc agaacatccg cacaggccag ccctgccgga cctgccccga
     8701 taatcgccac ccgcttgtcc actttggtga catgacttaa gtcaggacgc caacctttcg
     8761 ccaacgcctg atctgaaata tagcgttcaa tgttgccgat agttaccgcg ccgtgctcat
     8821 cgcgaatagt acaggcacct tcacacaaac ggtcttgcgg acaaacgcgt ccggtaattt
     8881 ccggcagggt gttggtctgg tgagaaagct cgacggcggc gtcgatgttt ccggctttca
     8941 ccagttcaat ccactgcgga atatggttat gcagcgggca ggtccattca caaacgctgt
     9001 gctcgccgaa cttaaggcag cgcgaggctt cccgttgtgc ctggtcggcg cgaaatggca
     9061 gataaatttc atcaaaaccg gttttgcgcg cttcaatcgc cagtttatcc ggctcgccac
     9121 gcgcgggcgt tgcctgcatt tgttcgactt tactcattac cggcatttct tgtgcagcgg
     9181 tactggcatg ccacggttga tgctcctgac gtgcggtgcg caagcggcgg gatttcgcca
     9241 tgccggagag tgcgacgtca gtgaccagtt gcagcgcgtc cgccgggcaa ttctcgacac
     9301 aggcaggacc gttttcgcgc cctgcacaaa ggtcgcattt atgcgccgtg gcttttactt
     9361 ttcctgccgc gacgggtgtc aggacgattt gcatcgtacc aaacggacag gccaccacgc
     9421 aggatttaca gccaatacac ttttgctgat tgacctgaat gctgtcatca acgtggctga
     9481 ttgcgccatt agggcagcta cgggcgcagg gcgcatcttc acaatggtga caggtcactg
     9541 cactacgttg ctgctgatgt ttgataaccg taattcgggg atgaaaatgg tgttggctca
     9601 ggacatgttg ctcatcattg tgagccatga cacaggcgat ttcacaagca tgacaaccca
     9661 gacattgctg actgttggcc ataataaaac gattcataac gaccttcttt tttggttgta
     9721 aaaaccttat tctttatatg agtgttgtta ttacccgact tacaggggaa ccggcaatgt
     9781 tcatgtgccc agaataagta actatttcgc tataaactgt ggcagatcaa ataatcccat
     9841 cactgactaa attgcgtttc agcgaactgg aacattaatg attttttgtg gagaagacgc
     9901 gtgtgattgt taaacgaccc gtctcggcca gtctggcccg ggcctttttt tacattgtgc
     9961 tgctgtcgat tctttccacg ggtatcgctc tgctaactct ggcgagcagt ttgcgcgacg
    10021 ctgaggctat caatattgcc ggatcgctgc gtatgcagag ttaccgcctg ggctacgact
    10081 tgcaaagtgg cagtccacaa ctcaatgcac atcgccagtt gtttcagcag gcactgcatt
    10141 caccggtatt aaccaacctc aacgtctggt atgtgccaga agcagtaaaa actcgctatg
    10201 cgcatctgaa tgccaactgg ctggagatga ataatcggct cagcaagggc gatttgccgt
    10261 ggtatcaggc caatattaat aattatgtta atcagataga cctgttcgtg ctggctttac
    10321 agcactacgc tgaacgcaaa atgctgctgg tggtggcgat ttccctggct ggcggtatcg
    10381 gtattttcac gctggtcttt tttactttgc gccgcatacg ccatcaggtg gttgccccgc
    10441 tgaatcagct ggttaccgcc agtcagcgta ttgaacacgg gcagttcgac tcgccgccgc
    10501 tggataccag tctgccgaat gagcttggtc tgcttgcaaa aacctttaac caaatgtcga
    10561 gcgagctgca taaattgtac ctttcgctgg aggcgtcagt agaagaaaag acccgcgatc
    10621 tccacgagac caagcgtcgt ctggaggtgt tgtatcagtg cagtgttccc aggcgctgaa
    10681 caccagccag attgatgtgc attgtttccg ccatattttg cagattgttc gcgacaatga
    10741 agcagctgaa tatctggagc tgaacgttgg ggataactgg cggattagcg aagggaagcc
    10801 aaaccctgag ctgccaatgc agattttacc ggtgacaatg caagagacgg tttacggcga
    10861 actgcactgg caaaatagtc acgtttcatc atcagaaccg ctgcttaaca gtgtttcatc
    10921 gatgctggga cgcggtttgt actttaatca ggcgcagaag cattttcagc agttattgtt
    10981 gatggaagaa cgtgcgacca tcgcccgcga attgcacgac tcgctggctc aggtgctttc
    11041 ttacttacgt atccagttga cgttactgaa gcgttcgata ccggaagata acgccaccgc
    11101 acaaagtatc atggccgatt ttttccaggc gttgaatgat gcttatcggc agttacgcga
    11161 gctgttgact actttccgcc tgacgctgca gcaggcggat ctcccctccg cgttgaggga
    11221 aatgctggat acgttacaaa atcaaaccag cgctaaactg accctcgact gccgtctgcc
    11281 aaccctggca ctggatgcgc aaatgcaggt gcatttgttg caaattattc gcgaagcggt
    11341 gctgaatgcg atgaagcacg ccaacgccag cgaaatcgcc gtcagttgcg tcaccgcgcc
    11401 ggacggcaat cacacggttt atatccgtga taacgggatt ggtatcggtg aaccgaaaga
    11461 acccgaaggt cattatggac tgaatatcat gcgcgaacgc gcggaacggc tgggcgggac
    11521 gctgactttt tcacaacctt ccggcggcgg cacgttagtg agtattagct ttcgctccgc
    11581 agagggtgag gggaagggga acacgccggc tgaggtgtaa ataattaccc tcacaccggc
    11641 cctctccctt taaaggcgag agggaaaaac gtgcccatat tgacctgtaa gcgagtgtgt
    11701 cagcaatctc atgataattt acattaactc ctttttttct ccacgattgg ctcgtacctt
    11761 gccgctacag tgaagcaagt caagcctaca acaatacgca gaaacacgag gtcctctttt
    11821 aatggcgaat ttctttattg atcgccccat ttttgcctgg gtgctggcaa tcctgttgtg
    11881 tctgacaggt accctggcga ttttttcatt gcccgttgaa caataccccg atctcgcgcc
    11941 accgaatgtg cgagtgaccg ctaactatcc cggcgcatcg gcccagacgc tggaaaacac
    12001 cgtgacccag gttatcgagc aaaatatgac cggcctcgat aatctgatgt atatgtcatc
    12061 tcagagcagt ggcaccggtc aggcatctgt cactttaagt tttaaagcag gcaccgatcc
    12121 ggacgaagcc gtgcagcaag tacaaaacca gctgcaatca gccatgcgaa agttaccgca
    12181 ggcggtgcaa aatcagggcg tgacggtgcg taaaaccggc gataccaaca ttctgaccat
    12241 tgccttcgtc tctaccgatg gctcgatgga taaacaggat attgctgatt atgttgccag
    12301 taatattcag gacccgttaa gccgcgtgaa tggcgtcggg gatatcgatg cctatggttc
    12361 gcaatattcc atgcgtatct ggctggaccc ggcgaaactc aacagtttcc agatgacggc
    12421 taaagatgtc actgatgcca ttgagtcaca gaacgcgcag attgcggttg ggcaacttgg
    12481 tggtacacct tccgtcgata agcaggcgct caacgccacc attaacgccc agtcactgct
    12541 gcaaacacca gaacagttcc gcgatatcac cttgcgggtg aatcaggacg gctcagaggt
    12601 aaggctgggc gatgtcgcca ccgtcgaaat gggggcggag aaatacgatt atcttagccg
    12661 cttcaatggt aagccagcct ccgggttggg ggtaaaactg gcctccagcg caaacgaaat
    12721 ggcgacagcg gagctggtgc tcaatcgtct cgacgagctg gcgcagtatt tcccgcacgg
    12781 actggaatac aaggtggcgt atgaaaccac ctcgtttgtt aaagcctcca ttgaagacgt
    12841 ggtgaaaacg ctgctggaag ctatcgctct ggttttcctc gttatgtatc tgttcctgca
    12901 aaacttccgc gccacgctga tacccactat cgccgtgccg gtggtgttga tgggaacctt
    12961 ctccgtactt tacgccttcg gttacagcgt caacacctta accatgttcg cgatggtgct
    13021 ggcgatcggt ctgctggtgg atgacgccat cgtggtggtg gaaaacgtcg aacgtattat
    13081 gagtgaggaa ggactcactc ctcgcgaagc cacacgtaaa tcgatggggc agatccaggg
    13141 ggcactggtc gggattgcga tggtgctttc ggcggtattt gtaccaatgg ccttcttcgg
    13201 cggcaccacc ggtgccatct atcgccagtt ctctattacc attgttgcgg cgatggtgct
    13261 gtcagtactg gtagcgatgc tcctcactcc ggctctgtgt gccacgctac ttaagccact
    13321 gaaaaaaggt gagcatcacg ggcaaaaagg tttttttgcc tggtttaacc agatgtttaa
    13381 ccgcaacgcc gaacgctacg aaaaaggggt ggcgaaaatt ctccaccgta gcctgcgctg
    13441 gattgtgatt tatgtcctgc tgcttggcgg catggtgttc ctgttcctgc gtttgccgac
    13501 gtcgttctta ccgctggaag accggggcat gtttactacc tcggtacaat tgcccagcgg
    13561 ttcaacccaa caacagaccc tgaaagtcgt tgagcaaatc gagaaatatt acttcaccca
    13621 tgaaaaagac aacatcatgt cggtgtttgc caccgttggt tctggccctg ggggtaacgg
    13681 gcaaaacgtg gcgagaatgt ttatccgcct gaaagactgg agcgaacgcg acagtaagac
    13741 cggcacctcg tttgccatta tcgagcgtgc aacgaaggcg tttaaccaaa ttaaagaagc
    13801 tcgcgttatc gccagcagcc caccagcaat tagcggtctt ggtagttctg caggttttga
    13861 tatggagttg caggaccacg ctggagcggg tcacgatgcg ctgatggcag cacgtaatca
    13921 gttgctggcg ctggcggcgg aaaacccgga gctaacccgt gtgcgccata acggcctcga
    13981 tgacagtccg cagttgcaga ttgatatcga ccagcgtaaa gctcaggcgc tgggcgttga
    14041 tatcgacgat attaacgaca cactgcaaac cgcctggggt tcgagctatg tgaatgactt
    14101 tatggatcgc ggtcgcgtga agaaagtcta tgtgcaggca gctgcgccgt atcgcatgct
    14161 gccagatgac atcaatctct ggtatgtccg aaataaagat ggcggcatgg tgcccttctc
    14221 tgctttcgcg acctcacgct gggaaacagg ctcgccgcgt ctggaacgct ataacggtta
    14281 ttctgcggtt gagattgttg gggaagccgc accgggggtc agtaccggta cggcgatgga
    14341 tattatggaa tcgttagtga agcagctgcc aaacggcttt ggtctggagt ggacggcgat
    14401 gtcgtatcag gagcggcttt ccggcgcgca ggctccggcg ctgtacgcca tttccttgct
    14461 ggtggtattc ctgtgtctgg cggcattgta tgaaagctgg tcggtaccgt tctcggtaat
    14521 gctggtcgtg ccgctggggg taatcggcgc gctgctggca acctggatgc gcgggctgga
    14581 aaacgacgtt tacttccagg tgggcctgtt aacggtcatt ggtttatcgg cgaaaaacgc
    14641 catcctgatc gtcgagtttg ctaacgagat gaaccaaaaa ggccatgacc tgtttgaagc
    14701 gacgctccac gcctgccgtc agcgtttacg cccgattctg atgacctcgc tggcatttat
    14761 cttcggcgta ttgccaatgg caaccagcac gggtgccggt tccggtggtc agcatgcggt
    14821 gggtactggc gtaatgggcg ggatgatttc agctactatt ctggctattt acttcgtgcc
    14881 gctgttcttt gtgctggtgc gccgccgctt cccgctgaag ccgcgcccgg aataagcaat
    14941 aaaaaaggcg acatgccaat gtgtcgcctt tttcaacttt ccgattaaga acctgctcag
    15001 cgggttcttg ctgtttatac tttgtctcag gaattactta cgaagcataa cttcgataaa
    15061 gtctttccag ttccccagtt cacgttcaat cataacaacc tctcttataa ttatgggtat
    15121 tctacggaaa caatataccg tggtgaagct aatttactcg attgctgcga tgactacctc
    15181 cgggggacaa accttatgta aatactatgg tcctgcggtg ataatttgta tgtgatacac
    15241 agcaacattt cgagatattc atacggcatc taatactgat ttaattctgg ttaaaataca
    15301 gacagataac aagatgaata ttcttaatgt ttacgttaaa aatgtttaat attatttaat
    15361 agttgttaat ttgaatactt cgataatgtt atattttctg ataatcattt gcaggcaaaa
    15421 tgttttcacc cttaaatgag tatttattct cataaatcga aaaaggattc attatggtta
    15481 cactttacgg catcaaaaat tgtgacacca ttaaaaaggc tcgccgttgg ctggaagcca
    15541 ataacatcga ctatcgtttt catgattacc gcgtcgatgg gctggacagc gaattattga
    15601 acggttttat caacgaatta ggctgggaag cgttactcaa cacccgtggt acaacctggc
    15661 gtaaactgga cgaaactacc cgcaataaaa tcaccgatgc ggcctctgcg gcggcattaa
    15721 tgactgaaat gcctgcaatt atcaaacgtc cattgctctg cgcgcctggt aagcctatgc
    15781 tgctgggttt cagtgattcc agttatcagc aatttttcca tgaggtgtag tctatgtcgt
    15841 gcccggttat tgagctgaca caacagctta ttcgccgccc ttccctgagt cctgatgacg
    15901 caggatgtca ggctttgttg attgaacgtt tgcaggcgat cggttttacc gttgaacgca
    15961 tggattttgc cgatacgcag aatttttggg catggcgtgg gcaaggtgaa acgttagcct
    16021 ttgccgggca taccgacgtg gtgccgcctg gcgacgccga tcgttggatc aatccgccgt
    16081 ttgaacccac cattcgtgac ggcatgttat tcgggcgcgg tgcggcagat atgaaaggct
    16141 cgctggcggc gatggtggta gcagcagaac gttttgtcgc acaacatccc aaccatacgg
    16201 ggcgactggc atttctgatc acttctgatg aagaagccag tgcccacaac ggtacggtaa
    16261 aagtcgtcga ggcgttaatg gcacgtaatg agcgtctcga ttactgcctg gtcggcgaac
    16321 cgtcgagtat cgaagtggta ggtgatgtgg tgaaaaatgg tcgtcgcgga tcgttaacct
    16381 gcaacctcac cattcatggc gttcaggggc atgttgccta cccacatctg gctgacaatc
    16441 cggtacatcg cgcagcacct ttccttaatg aattagtggc tattgagtgg gatcagggca
    16501 atgaattctt cccggcgacc agtatgcaga ttgccaatat tcaggcggga acgggcagta
    16561 acaatgttat tccgggtgaa ctgtttgtgc agtttaactt ccgcttcagc accgaactga
    16621 ctgatgagat gatcaaagcg caggtacttg ccctgcttga aaaacatcaa ctgcgctata
    16681 cggtggattg gtggctttcc gggcagccat ttttgaccgc gcgcggtaaa ctggtggatg
    16741 cggtcgttaa cgcggttgag cactataatg aaattaaacc gcagctactg accacaggcg
    16801 gaacgtccga cgggcgcttt attgctcgca tgggggcgca ggtggtggaa ctcgggccgg
    16861 tcaatgccac tattcataaa attaatgaat gtgtgaacgc tgccgacctg cagctacttg
    16921 cccgtatgta tcaacgtatc atggaacagc tcgtcgcgtg atgagtggtt ctgcaagagg
    16981 aaataagcat ggactggctg gctaaatatt ggtggattct ggtgattgtc tttttggtag
    17041 gcgtcctgct gaacgtgatt aaagatctca agcgcgtcga ccataagaaa tttctcgcca
    17101 acaagccgga gcttcccccg catcgtgatt tcaacgataa gtgggacgat gacgacgact
    17161 ggccgaaaaa ggatcaaccg aagaagtaag ctgagtgctc aaccgtaggc cggataaggc
    17221 gttaacgccg catccggcaa tggtgaacga tccctgatgc gacgctcacg cgtcttatca
    17281 ggccaggtaa tctgtctttt acatcatttc aatcacatca tcatcgccag gcttaccgcc
    17341 acttagcgct tggtcgaaat aatgtttcgg aatggtataa cgcaagtgat ccagggcaaa
    17401 ttgcatactg cgattgtcga tcgcatggcc taaatcctcc acgatatcca gcgtcacgtc
    17461 accaccggca ctgattaacg cttcctgcgc cgcaactgca tgcgccagat cgataaccgg
    17521 atcttcacca ccatgaatca agtgaattgt cgtggctgta gacgcggtct ccggcaggct
    17581 cgcatagcgt ccgttaaagg caatgacgcg cgaagcaagg cccggttcgg ctttaatgct
    17641 ctccagcgcc ataatcgcgc cttgtgagaa accaattagc gccgtggcgt tagcccctac
    17701 tccgctctgt ttctgccagt agcgtaccgt ttcaataaac gtcggcatga tggcatccac
    17761 acgcgcctgg cgattatctt ccgtaatacc ctgcactgaa aaccactgac gccccgccgg
    17821 attaccgctc ggctccgcgc caccgacact caccaccagc gcatcaggaa acagcggtgc
    17881 aaaccagctg cctatttccc ccattgccac cgggttatcc ccgacaccgt ggaaaagcag
    17941 taacaactgt tgtgcaggtt tatccgggct ttgaacaaca aaatggtcat gtttcatggc
    18001 gaactcctta actgatgtca ttaattttac gccgctgact cacaatgacc atgggagaaa
    18061 attgaaggag tcagtgaaaa aattgccatt gcgttatgcg atcgcgcaga cgctcagtgc
    18121 gaacctcatt tagtgcgaac agcgcctgcg cggcctcttc ccgctggcga accagtaaca
    18181 tcttgcggcc tgaaagttta agtgtggtac ataactgcgc gtcactggcg tttttctgta
    18241 aacgcccacg cagcgccggt aatgccagtt cactggtttg tagcagacgc aataagcaac
    18301 ctaacgatgt taatagcgga cgatgagcga aagcaaaacc ggccagttca agccagtcgt
    18361 cgtcagaaag gatggcatcg tttagtggat caacaggaag catttcgcca ttccactgcg
    18421 cgagagcttg cgcatcgcga cgtaaacggt aatgctcacg ttcagccagc tgtttacccg
    18481 catcactcat cggtaacagc gccatcgccg tatagcaacc gctgctggct tcccgatgat
    18541 tacccatccg caccagcaca aaaccgcagc gttgccagaa acgccataac tccccagtgt
    18601 aaccaaaact caccgaaaga tagtcgaggt catgcgtata ttgcaaagct ccagcaataa
    18661 gttgtcgccc tgtgccttcc cgctgacgag ccggatgaac tgctatccgg ctaacccgcc
    18721 ttccgcgcaa tgtcgccgcc agtggattgc tgccgtgcgc cgccagcgac tgggccacca
    18781 gattaccccg cgggcgacga aaacctgccc ataccgcctg actgagttct tgagataatc
    18841 cgccctcatc caccagccac aacgccccgg caatctcgtt ttcgccagcc gcctgtaaaa
    18901 aatgttgccc tggtgcatcc atcatccggc gtaaatccag cggcgaggtc cggtagtgcg
    18961 caccagacaa taactgataa acctttaacg gcgtttctgg ctcgcttcgc cataacgttt
    19021 gttcaaatgc ggaaatgacg atattgcctt gtggtgtatg ggtgaagttt tcatcgttaa
    19081 aaaccagtgc ctcactaacc attttttcca gcgggcatcc ctgtgcccag cggatcggct
    19141 gttgcagttc aaaacggtgt aaatgcggaa agcgagcgca aaatttcagc aaaaaaccac
    19201 gtccggtacc ttcgtagccc tgcaccgtag tggttaacaa cgttcgagga aaacgcgata
    19261 ccagttgatg caacaacggc gcaggtatgg ctgcggcttc gtcgaccacc agccagtcgg
    19321 cttgctcatc gctggctaac aaggcatccg gtgcaataaa gcgaaacttc tcgcccgcaa
    19381 attgtgccag tacatccgtt gccgcttttg cgggcgcggt gacaatcgca ctgcccgcaa
    19441 tacgagaaat gagttgccct gccaacgccg acttaccgcg cccacgcgca gccgttaccg
    19501 ctgccacgcc cggcggcatg gtcagtagct gctgtaagag ttgctgttgt tctggttgtg
    19561 gtgcgccggt cgcggggtgc cagtcagtac ggggagtaaa atgcgccaac gagaacggct
    19621 ggttttgccg ccagaggata gcgtcgttat ccgccgtaag tacgcgtttg agatgctgga
    19681 caaaatgcgg cgtcgcaata gggtcagggc aatcactcca gcgcagcgag tcggcatcag
    19741 gttggttttc ccactcttcc catacaggga gtaacaaaac cagccagctt cccgctttca
    19801 acgttccgct aagtgctgca aaggcagcgg catcaaagcc gtggcgggcg tcgaataccg
    19861 catgccggaa ctcgcgccca agtaaagttt gtagcgccga gggtgaacag tggttttcag
    19921 catctagctg cggcgaaatc cacagccagt cgccaggtaa ggcatcacgc aacttaagag
    19981 catgatcaaa acaccaaccc tcttccccgc tcaacaccag caagcggcag atcccttcac
    20041 gtttcatttg cgctgttaat gtgtaaagcg cagtcagttc agccatccct gccccggaaa
    20101 ttaaatgctt ttaccaaaag tattgcattg tgccggatcg ccgctgtcga aaccacgttt
    20161 aaaccagctg tagcgttgct gagaagtgcc atgtgtgaaa ctgtctggta ctactcgccc
    20221 ctgactttgc tgttgtaaac ggtcatcgcc gatggcctgc gccgcattca gcgcctcttc
    20281 cagatcgcca gtttccagaa cgccttgctg ctgcatacta tgcccccaga caccggcaaa
    20341 acagtcggcc tggagttcca tacgcacaga taagcggttt acttccgcct gcgtcgcgtt
    20401 ttgttgcagt tgacgaactt tcggctcgat gcctaacagt ttctgcacat gatgaccgac
    20461 ttcatgggcg ataacgtacc cctgggcaaa atcgccatcc gcgccaagtt tgtctttcat
    20521 gtcatcatag aaggagagat cgatataaac tgtgccatcc gccgggcaat agaacggccc
    20581 cataatggac tggcccgccc cgcagccggt gcgcgtcatt ccacggtaca tgaccagctt
    20641 cggttgctga taggtcttac ccatcttttc gaactgttgt ccccaggtgt cttccgtggt
    20701 tgccagaatc accgaggtga attttgcggc ttcgtcttca ttagggctaa ttgaccgcgt
    20761 tgattgttgt tgagaaaccg gctgcccggt catcaacccg gttaaatcaa cgccatagta
    20821 gcctgcaacc agcacgacta tcagtaaaat cagcccgcct ttaccgctcg gcagacggaa
    20881 accgggaccg cccattgacg gaccgccaga gctgttgcgc ctgtcttcaa cattgtcact
    20941 ttcacgtcgc ccttgccaac gcatagatac ctcaacaata tattcattat agaaatgatc
    21001 gtaggtggtt cagaggaaga ttaccacagg aaagcgggat gaaatggcgg aggaatgagc
    21061 gttcaatgca tatattccga gagcggttgt cttgccgtgc cagctgcacg gcaagatgat
    21121 gaacagaaaa atcagtccag ctgtacaccc aggcggcggg cgacggcttc ataggcttcg
    21181 atcaggccac cgaggctctg gcggaaacgg tctttgtcca ttttctccag tgtttctttg
    21241 tcccacaggc ggctgccgtc cggggagaac tcatcgccca gtaccacttc acctttgtac
    21301 agaccaaact ccagcttgaa gtcgaccaga atcagaccag catcatcgaa cagttttttc
    21361 agcacgtcgt tcgctttgta ggtcagctct ttcatacgcg ccaggttctc tttgctcacc
    21421 cagccaaagg tttcgcagta agattcgttg accatcggat cgtgcatggc atcgtttttc
    21481 aggaacagat cgaacagcgg cgggttcagc tcaatacctt cttcgattcc aagacgtttc
    21541 accagagagc cagcagcacg gttacgcacg acacactcaa ccggcaccat atccagcttt
    21601 ttcaccagac attcggtatc ggagagcaga cgctccattt gagtcgggat acccgcttca
    21661 gccagtttgc tcataatgaa gtagttgaac ttgttgttca ccataccttt gcgatcaaac
    21721 tgctcaatgc gcgcgccatc ccctgctgac gtatcattgc ggaattcgag caccaacagg
    21781 tccgggtttt ccgtgctgta tacggttttc gctttaccac gatacaactc agcttgcttt
    21841 tgcatcttta tcactcctgg gtgtgaatta acgttttaaa atcttttgct gtctggtgtg
    21901 ccggatgttt tgtcggatgc ggcgtgaacg ccttatccga actacgtccg acaaacaaat
    21961 ttcgtgcgaa ttaccgctaa aatcgcgttt tcctgtcgac gcacacgttt gcgtatcata
    22021 tcagaaaaaa gggccggatg attccagccc tgtattttta cttgctaaac gcagcctgga
    22081 agacagctac cagcgcgtcg ttctgactct gagtcagagt atgacctttc ggatcgatga
    22141 actgtaggct gctgcggtta tctaaatcgc caacctgcag tttatagtca ccggatgcca
    22201 ggcctggatc gctcgcgccc agttcctgcc agtcgctgtc agacagcggc ttataagtta
    22261 cggccatgtt gccctgcgaa cgggtgctgt cggtcacttt catgcccact ttttccagcg
    22321 ccgctggcag acgttgccaa accacattga acggcccgcg tacgaccagc attggtaaac
    22381 cggtgtcatc agctgcactt tgtacgtcca tagtggtgga ggcacgattt tgcgcagcgt
    22441 tcgcggcgtc agtggcagat ttatccagac cggcggaaat aacgttcatc atctccgtgc
    22501 tgtaacgctg catggaagcc gcgtctgcaa ccggtttgcc cgcctgttcc aggttcagca
    22561 gtttaaccgt aaccgcctgc tgataaccct gcggcttaac agagatttga taacgaccac
    22621 gatactgctc gtcttcgtcc agacggttcc attgtaccca atcggtggtc agtgtctgac
    22681 caacatcatc acgttgggtg atggtgtagt ttttcgcctg cagcacgcta accacctgcg
    22741 gccacagagt attgccacga ccattttcca ccagcaatga agcggtatcg cccgtgaact
    22801 gggtacgcgc gccagaaacc agtgccaacg gctgggctgg tggacgaatg tccagcgcct
    22861 taccgacagc accactaccg ttggtcaccg ggattgcata atcaccggag gtcaccggca
    22921 aaatcattcc agccggggca tgaagctccg caagcggtgc cgcttccagg taggcttcat
    22981 caccactgac ctgacgctta tagcgtgagt cagaactaca ggcagcgagt aataaaacaa
    23041 gcgaaacacc cgcaaccttt gccaggcgcg acttttgaac agagtaagcc atcaaatctc
    23101 cctaaacttt acagcaaacc ggcatgctta agcgccgctc tgaccgtctc acgaccactg
    23161 tcggtgattg gtgtcattgg caggcgcagc gtatcggtcg ccacaagacc cagttcctta
    23221 catgcccatt tcaccgggat tggattgggt tcgacaaata gtttgttgtg taatggcatc
    23281 agacgctgat taataacgcg tgcctcggca aaatgccctt ctgctgccag tttgcacatc
    23341 tgggccatat cacgcgctgc gacgttagcc gtaacggaaa taactccatg accgcccaat
    23401 tgcatgaagt ccagcgcgct cgcatcatcg ccgctcagca gaacaaaatc atctgaaacc
    23461 agctctttga tctggtttac acgcgttaag ttccctgttg cctctttgat tccgataata
    23521 ttttttactt tcgccagacg gcccaccgtt tccgggagca gatcgcagcc agtacgggac
    23581 ggcacattat acagaatttg cggcaggtca gtatgctcag cgatggcttt gaaatgctga
    23641 tacaaacctt cttgcgacgg acgattgtag taaggggtta ccgtcaggca gccgacgata
    23701 ccactgtcat tgaagcgctg cgtcaggcta atggcttccg cagtagcgtt agcgccggtc
    23761 ccggcaatta ccggaatgcg cccatcagcc agatccagcg tcatcatcac cacatcagca
    23821 tgttcgtcat gatttaaggt agcggactcg ccagtggtgc caacagaaac gatcgccgaa
    23881 gtaccgctgg cgacatgata atcaatcagt tttttcaagc tagcccgaca gacattacct
    23941 ttttcatcca tcggagtaac aatcgcgaca atacttcccg tgaacatggg ccatcctctg
    24001 tgcaaacaag tgtctcaatg gtacgtttgg tatggcatta aaagcaagca gacagaaccg
    24061 ttctgattgt tgtatgcatg atttttttat gctttcctta agaacaactc accccttaaa
    24121 ggaataacca gtttgacact gtcatcgcaa cattatctgg tgatcactgc gttgggtgcc
    24181 gatcgccctg gaattgtgaa caccatcacc cgtcatgtca gtagttgcgg ctgtaatatt
    24241 gaagacagtc gcctggcgat gctgggagaa gagttcacgt ttatcatgct gctttccggt
    24301 tcatggaatg ccatcactct gattgaatca acgttaccgt tgaaaggtgc cgaactggat
    24361 cttttaatcg tgatgaagcg cacgacggcg cgtccgcgtc cgccaatgcc agcatctgtc
    24421 tgggttcagg tcgatgttgc agactccccg catttaattg aacgcttcac agcacttttc
    24481 gacgcgcatc atatgaacat tgcggagctg gtgtcgcgca cacaacctgc tgaaaatgaa
    24541 cgggctgcgc agttgcatat tcagataacc gcccacagcc ccgcatccgc ggacgcagca
    24601 aatattgagc aagcgttcaa agccctatgt acagaactca atgcacaagg cagtattaac
    24661 gtcgtcaatt attcccaaca tgatgaacag gatggagtta agtaatgaat ccactgaaag
    24721 ccggtgatat cgcaccgaaa tttagcttgc cggatcaaga cggagaacaa gttaatttga
    24781 ccgacttcca gggacagcgt gttctggttt atttctaccc gaaagccatg acccccggct
    24841 gtaccgtaca ggcctgcggc ttacgcgata acatggatga gttgaaaaaa gcgggcgttg
    24901 atgtgctggg tatcagcacc gataaacccg aaaaactctc ccgttttgcg gaaaaagagc
    24961 tgcttaactt tacgctcctg tctgatgagg accaccaggt gtgcgaacaa ttcggcgtct
    25021 ggggtgaaaa gtccttcatg ggcaaaacct acgatggtat tcatcgcatc agcttcctga
    25081 ttgacgctga tggcaaaatc gaacatgtct ttgacgattt caaaaccagc aatcaccacg
    25141 acgttgtgct gaactggctg aaagaacacg cctgattact ttgctccatt ccgtgctggc
    25201 tgcgcttgcg gccagcatac ctcacttctc gtgatcaaga tcacattctc gctttcccct
    25261 gcgacacggg tgtcgaatcc attttttgct gaacgttaat gaccatcatt tttgtaccgt
    25321 tcagaatcca gttaatacat aacttattga atagattgag ttaatcagaa tggcatcctt
    25381 tatacaatat gaaatgcaat gtttcatatc attttcaagg agccgacatg aaccgctttg
    25441 tggtggccga accactgtgg tgtacaggat gtaatacctg tctcgctgcc tgttcggacg
    25501 tgcataaaac gcaaggttta cagcaacacc cgcgcctggc cctggcgaag acgtcaacaa
    25561 tcactgcccc tgtcgtgtgt catcactgtg aggaagcccc ttgcctgcag gtctgcccgg
    25621 tcaatgccat ctctcagagg gatgatgcga tccaactcaa cgaaagcctc tgtattggct
    25681 gcaagctttg cgccgtggtc tgcccatttg gcgcaatcag cgcttcagga agccgtccgg
    25741 tgaatgccca tgcgcaatat gtttttcagg ctgaaggctc actcaaagac ggcgaagaaa
    25801 acgtgccgcc ccaacatgcg ttgctgcgct gggaaccggg tgtccagacc gtcgcggtga
    25861 aatgcgacct gtgtgatttc ttgccagaag gtccggcctg cgttcgcgct tgcccgaatc
    25921 aggcgttacg gctgatcacc gacgatagcc tgcaacgtca gatgaaagaa aaacagcgcc
    25981 ttgccgcaag ctggtttgcc aatggcgggg agtctcccct ttccctcact caggagcaac
    26041 actaatggat gccctgcaat tattaacctg gtcgctgatc ctctatctgt ttgccagtct
    26101 ggcttcgctg tttttactcg gtctggacag actggctatt aagctttccg gcatcacatc
    26161 gctggtgggc ggcgtgattg gcatcatcag cggaattacg caattacatg ctggtgtaac
    26221 tttagtcgcc cattttgcca ccccttttga ctttgccgat ttaaccctgc gaatggatag
    26281 cctctcggca tttatggtgc tagttatctc cttgctggtg gtggtttgtt cgctctattc
    26341 attgacttat atgcgcgaat acgagggcaa aggcgcggcg gcgatgggct tctttatgaa
    26401 tcttttcatc gcatcgatgg ttgccctgct ggtgatggac aacgcttttt ggttcatcgt
    26461 gctgtttgaa atgatgtcgc tgtcttcctg gtttctggtc attgccaggc aggataaaac
    26521 gtcgatcaac gctggcatgc tctacttttt tatcgcccac gccggatcgg tgctgattat
    26581 gatcgccttc ttgctgatgg ggcgcgaaag cggcagcctc gattttgcca gtttcgcacg
    26641 ctttcacttt ctccggagct ggcgtcggcg gtgtttctgc tggccttttt cggttttggc
    26701 gcgaaagccg ggatgatgcc gttgcacagc tggttgccgc gcgctcaccc tgccgcacca
    26761 tcgcacgctt cggcgttgat gtctggcgta atggtcaaaa tcggtatttt cggcatcctg
    26821 aaagtagcga tggatctgct ggcgcaaacg ggtttgcctc tgtggtgggg cattctggtg
    26881 atggcgatcg gcgcaatctc cgcgctcctg ggcgtgctat atgcgctggc ggaacaggat
    26941 atcaaacggc tgctggcctg gagtaccgtc gaaaacgtcg gcattatttt gctggcagtc
    27001 ggtgtggcga tggacggtct gtcactgcac gacccgctgc tcaccgtggt tggactgctc
    27061 ggcgcactgt ttcatctgct caaccatgcg ctgttcaaag ggctgctatt tctcggcgcg
    27121 gacgcgatta tttcgcgttt gcatacccac gacatggaaa aaatgggggc actggcgaaa
    27181 cggatgccgt ggacagccgc agcatgcctg attggttgcc tcgcgatatc agccattcct
    27241 ccgctgaatg gttttatcag cgaatggtac acctggcagt cgctgttctc actaagtcgt
    27301 gtggaagccg tagcgctaca acttgcgggt cctattgcta tggtaatgct ggcagtcact
    27361 ggtgggctgg cagtaatgtg cttcgtcaaa atgtacggta ttactttctg tggtgcgccg
    27421 cgcagtacac acgctgaaga ggcacaggaa gtgccaaata cgatgatcgt cgccatgcta
    27481 ctgctcgcgg cactctgcgt attaattgcg cttagtgcca gttggctggc accgaagata
    27541 atgcatattg cccatgcgtt taccaatacc cctcccgtca ctgtcgccag cggaatagca
    27601 cttgtacccg gcacgtttca tacacgggtc actccttcat tactgttgct gttactactg
    27661 gcgatgcctt tgctgccagg cctttactgg ctgtggtgtc gttcgcgccg cgcagcgttt
    27721 cgtcgcacag gagatgcctg ggcatgcggc tacggctggg aaaacgcgat ggctccgtca
    27781 ggcaatggcg tgatgcagcc gctgcgtgtg gtcttctctg cgttatttcg tctacgacaa
    27841 cagctcgacc ctacgctgag gctaaataaa ggtcttgcgc acgtcaccgc cagggctcag
    27901 agcacagaac ccttctggga tgagcgggtg atccgcccca tcgtgagcgc cacccaacgg
    27961 ctggtcaaag aaatacagca tctgcaaagc ggcgactttc gtctctattg cctgtatgtg
    28021 gtcgccgcac tggttgtgct gctaatcgct attgccgtct aaggaaatca ccatgagaca
    28081 aactctttgc gacggatatc tggtcatttt tgcgttagca caggccgtga ttctgctgat
    28141 gctaacccca ctttttacgg gtatttcccg gcagatacgc gcgcgtatgc actcccgccg
    28201 cgggccgggg atctggcagg attatcgcga tatccacaaa ctgtttaaac gccaggaagt
    28261 tgcgccgaca tcttcaggtc tgatgttccg cctgatgccg tgggtattaa tcagcagcat
    28321 gctggtgctg gcgatggcct taccactatt tattaccgtt tccccttttg cgggcggcgg
    28381 cgatctgatc acccttatct atcttcttgc cctgtttcgt tttttctttg ctctttccgg
    28441 gctggatacc ggaagtccgt ttgcgggagt cggtgccagt cgcgagttga cgctcggcat
    28501 tctggtcgaa ccaatgctta ttctctcact gctggtattg gcgctgatag caggttccac
    28561 gcatatcgag atgatcagca atacgctggc tatgggctgg aactcgccgc taaccaccgt
    28621 actggcgtta ctggcctgtg gttttgcctg cttcattgag atgggaaaaa ttccctttga
    28681 tgttgctgaa gcagaacagg aattacagga aggcccgctg accgaatatt ccggtgccgg
    28741 gctggcgcta gcgaaatggg ggctggggct gaaacaggtc gtgatggcag cactgtttgt
    28801 ggccctgttt ctgccctttg ggcgcgcgca agaactttct ctcgcctgcc tgctgacttc
    28861 acttgtcgtt acgctgctca aggttttgct gatttttgta ctggcctcaa tcgcagaaaa
    28921 cacgctggca cgcgggcgtt ttttactcat tcaccatgtg acctggcttg gcttcagcct
    28981 tgctgcgctt gcatgggtct tttggttaac cggtctgtaa ggagcactga cggaatatgg
    29041 aaaatcttgc tctgacgacg ttaatgctgc cttttatcgg cgcactggtc gtttcgtttt
    29101 cgccacaacg tcgggccgcc gaatgggggg ttttgttcgc cgcgctgacc acgctgtgca
    29161 tgttgtcgct gatctccgcg ttttatcagg ccgataaagt tgccgtcacg ttgacgttgg
    29221 tcaacgtggg ggatgtggca ttgtttggcc tggtcattga tcgcgtgagt acgctgattc
    29281 tgtttgtggt ggtgtttctc ggtttgctgg tcacgatcta ctccacgggt tatctgacgg
    29341 ataaaaatcg cgaacacccg cataacggca cgaatcgtta ttacgcattt ttgctggtgt
    29401 ttatcggcgc gatggcggga ctggtagtct cctcgacgct gctcggtcag ttgttgtttt
    29461 ttgaaattac gggcggctgc tcctgggcgt tgatcagtta ttaccagagc gataaagcgc
    29521 agcgttcagc actaaaagcg ttgcttatca cccatatcgg ttcgttgggg ttgtatcttg
    29581 ccgccgccac gctgtttttg cagaccggaa cgtttgtgct tagcgcgatg agcgagttac
    29641 atggcgacgc acgttatctg gtttatggcg gcatcctgtt tgccgcgtgg gggaaatcgg
    29701 cccagctacc gatgcaagcg tggctaccgg acgcaatgga agcgccaaca ccgatcagcg
    29761 cctatctcca cgccgcatcg atggtgaaag tgggcgttta catttttgcc cgcgccatta
    29821 tcgacggcgg caatatcccg catgtgattg gcggcgttgg catggtcatg acactggtca
    29881 ccattcttta tggctttctg atgtatttgc cacagcagga tatgaagcgg ttgctggcct
    29941 ggtcgaccat cactcaactt ggctggatgt tcttcggctt gtcgctctcc atcttcggct
    30001 cgcggctggc gctggagggt agcatcgcct acatcgtcaa ccacgcgttc gctaaaagcc
    30061 tgtttttcct tgtagcaggt gcgctgagtt acagctgcgg cacgcgcttg ttgccgcgtc
    30121 tgcgtggcgt attgcacacc ctgccgttgc caggcgtggg tttctgcgtg gcagcgctgg
    30181 caattaccgg tgtgccgccg ttcaacggct tcttcagtaa attcccgctg tttgccgccg
    30241 gttttgcgtt gtcagtggag tactggatcc tgctgcccgc catgattctg ctgatgattg
    30301 aatcggtcgc cagtttcgcc tggtttattc gctggtttgg tcgcgtcgtg ccgggcaaac
    30361 cgagcgaggc cgtcgccgat gccgcaccgc tgccagggtc aatgcgcctg gtgttgattg
    30421 tactgattgt gatgtcgctg atttccagcg taatcgccgc gacctggttg cagtaaggag
    30481 atgatgaatg accggttcta tgatcgtaaa taatctggcg ggactgatga tgctgacatc
    30541 gctgtttgtg attagcgtca aaagctatcg cctgtcatgc ggattttacg cctgccagtc
    30601 gctggtgctg gtgtctattt tcgccactct ctcgtgcctg ttcgccgcag agcaactgct
    30661 gatctggtcc gccagcgcct ttatcaccaa agtgctgctg gtaccgttaa tcatgactta
    30721 cgctgcacga aatattcccc agaacatccc ggaaaaagcg ttattcggtc cggcaatgat
    30781 ggcactgctc gcggcgttaa ttgtcctgct ttgcgcattt gtcgttcagc ccgtgaagct
    30841 accgatggct accgggctga aaccggcgct ggcggtagcg ttaggtcatt ttctgcttgg
    30901 cctgctgtgc attgtcagcc aacgcaatat cctgcggcaa atttttggtt actgcctgat
    30961 ggaaaacggc tcccatctgg tgctggcgct tcttgcctgg cgagcaccgg aactggtgga
    31021 aataggtatc gctaccgacg ccatcttcgc cgtcattgtg atggtgttac tggcaagaaa
    31081 aatatggcgt acccacggca cgctggacgt gaacaacttg accgcgctga agggataatg
    31141 agatgagtta ttctgtgatg ttcgctttac tcctgctcac gccgctgctt ttttcgctgc
    31201 tctgttttgc ctgccggaaa cgggggctct ctgcgacttg tacggtgact gtattacata
    31261 gcttagggat cacactgctg ctgattctgg cactctgggt ggtccaaact gccgctgatg
    31321 caggagaaat attcgctgcg ggactgtggc ttcatattga tggtctgggc ggtttgttcc
    31381 tcgccattct tggtgtgatt ggctttctca ccggtgttta ctcgattggc tacatgcgtc
    31441 atgaagtggc acacggcgag ctttcacccg ttacactgtg cgattactac ggtttcttcc
    31501 atctgttttt gttcaccatg ctgctggttg ttaccagcaa taacctgatt gtgatgtggg
    31561 cggcgatcga agccaccacc ttaagctcgg cgtttctggt aggcatttac ggtcagcgtt
    31621 catcgctgga atcagcatgg aagtacatca ttatttgtac tgttggtgtc gcttttggtc
    31681 tgttcggtac cgtgctggta tacgccaacg ccgccagcgt tatgccgcag gcagaaatgg
    31741 cgatattctg gagcgaggtt cttaagcaat cgtccttgct tgacccaaca ttaatgctgt
    31801 tggcctttgt gtttgtgcta attggctttg gtaccaaaac cgggctattt cccatgcacg
    31861 cctggctgcc ggatgctcac agtgaagcgc cgagtccggt cagcgccctg ctctccgccg
    31921 tattgctgaa ctgcgcgctg ttggtgctga ttcgctatta catcattatt tgccaggcca
    31981 tcggcagcga tttccccaac cggttgttgc tcatcttcgg catgttgtcg gttgccgtgg
    32041 cggcattttt cattctggta cagcgggaca ttaagcgtct gctggcgtac tccagcgtgg
    32101 agaacatggg gctggtcgcg gtggcgctag gcattggcgg gccgctggga atttttgccg
    32161 cgctgctgca caccttaaac cacagtctgg caaaaacgct gctgttctgc ggttccggca
    32221 atgtactgct caagtacggc acgcgcgatc tcaacgtcgt ctgtgggatg ctcaaaatca
    32281 tgccatttac cgccgtgctg tttggcggcg gtgcgctggc gctggcgctg gcagggatgc
    32341 cgcccttcaa catttttctt agcgaattta tgaccgttac cgcaggactg gcacgtaatc
    32401 acctgctgat tatcgtcctg ctgttattgc tgttaacgct ggtgctggcg ggcctggtac
    32461 ggatggctgc gcgggtgtta atggcgaaac cgccgcaggc cgttaaccgg ggtgatctcg
    32521 gctggttgac cacctcgcca atggtgattc tgctggtcat gatgctggcg atgggaacgc
    32581 atattccaca acctgtcatc aggatcctgg cgggcgcttc cactatagtc ctctcaggga
    32641 cgcacgacct gcctgcacaa cgtagcacct ggcatgattt tttgccttca ggcaccgcat
    32701 ctgtttcgga gaaacacagt gaacgttaat tcatcgtcaa atcgtggcga agcgattctc
    32761 gccgccctga aaacgcagtt ccccggcgcg gtgctggatg aagagcgaca aacgcctgaa
    32821 caggtcacca ttacggtgaa aatcaatctg ctgcctgacg ttgtacatta tctttattat
    32881 caacatgatg gctggcttcc agtcctgttt ggcaacgacg agcggacact taacggtcat
    32941 tacgcggttt attatgccct ttcaatggaa ggggccgaaa aatgctggat cgtggtgaag
    33001 gcactggtcg atgccgacag tcgggagttt ccgtcagtca caccgcgcgt ccctgccgcg
    33061 gtctggggcg agcgagaaat tcgcgatatg tacgggctga ttccggttgg cctgccggat
    33121 cagcgtcgcc tggtgttgcc cgatgactgg ccggaagata tgcatccgct gcgcaaagat
    33181 gcgatggatt atcgactgcg ccctgaaccg acgacagatt ccgaaacgta tgcgtttatc
    33241 aatgagggca acagcgatgc gcgggtgatc cctgtcggcc cgctgcatat cacctcctat
    33301 gaaccgggtc acttccgctt gtttgtggat ggcgagcaaa ttgtcgatgc tgattaccgc
    33361 ctgttttatg tccatcgcgg catggagaaa ctggcagaaa cgcggatggg ctacaacgaa
    33421 gtgaccttct tatcggaccg cgtgtgtggg atttgcggtt ttgcccacag tgtggcctat
    33481 accaactcgg ttaaaaatgc actggggatt gaggtgccgc aacgagcaca tactattcgc
    33541 tcgattctgc tggaagtcga gcggctacac agtcatttgc tcaaccttgg cctctcctgc
    33601 cattttgttg gttttgatac cggctttatg caatttttcc gcgtgcggga aaagtcgatg
    33661 acgatggcgg aattgctgac cgggtcgcgt aaaacctacg gtctgaatct gattggtggt
    33721 gttcgccgcg atattctcaa agagcaacgt ctgcaaacgc tgaaactggt gcgcgagatg
    33781 cgcgccgacg tgtcggagct ggtagagatg ctgcttgcta cgccgaatat ggaacaacgc
    33841 actcagggca ttggcattct cgaccgacaa atcgcccgtg attatagccc tgtagggccg
    33901 ctgatccgcg cagtggtttt gcccgtgatt tgcgctttga tcacccctac gccgactacg
    33961 gcaatattcc aaaaacactg tttaccttta ccggcggcga tgttttctcc cgcgtgatgg
    34021 tccgtgtcaa agagacgttt gattcgctgg caatgctgga atttgccctc gacaacatgc
    34081 cggatacccc actgctgacc gaaggcttta gctataaacc tcacgcattc gcgctgggct
    34141 ttgttgaagc gccacgcggt gaagacgtgc actggagcat gctcggtgat aaccaaaaat
    34201 tgttccgctg gcgctgtcgt gccgccacct acgccaactg gccggtgttg cgttacatgc
    34261 tgcgcggcaa taccgtttct gacgcaccgc tgattatcgg tagccttgat ccctgctact
    34321 cctgtaccga ccgtgtgacg ctggtagatg tgcgcaagcg ccagtcaaaa accgtgctgt
    34381 ataaagagat cgaacgctac ggcattgatc gtaaccgttc gccgctgaag taaggacaga
    34441 agatgctgaa gttactgaaa actattatgc gcgccggaac cgcgacggtg aaatatccct
    34501 tcgcgccact ggaggtcagc ccaggctttc gcgggaaacc ggacctgatg cccagccaat
    34561 gtattgcctg cggtgcctgc gcctgtgctt gtccggcaaa tgcgctgact atccagaccg
    34621 acgaccagca aaattcgcgc acctggcagc tctatctggg gcgttgtatt tactgcggac
    34681 gttgtgaaga agtgtgcccg accagagcca tccagcttac caataacttt gaactgaccg
    34741 tcacccataa agccgatctc tatacccacg cgacgttcca tctacaacgt tgcagccgtt
    34801 gcgaacgccc gtttgccccg caaaaaaccg tcgcactggc tgctgaattg ttggcacagc
    34861 aacaaaatgc tccacaaaac cgcgaaatgt tatgggcgca agcgagcgtc tgcccggagt
    34921 gcaaacaacg cgcgacgctg atcaacgacg atacagatgt accgctggtg gctaaggagc
    34981 agctatgagt ccagtgctta cgcaatatgt cagccagccc atcacgctgg acgagcaaac
    35041 gcaaaagatg aagcggcatt tgctacagga tatccgtcgc tcggcttacg tttatcgcat
    35101 cgattgcggc ggctgcaacg cctgtgaaat cgaaattttt gctgccatca cccccgtatt
    35161 cgacgcagaa cgttttggca ttaaggttgt ttcatcaccg cgtcacgccg atattttgtt
    35221 atttactggc acagtcaccc gggcgatgcg tatgcctgca cttcgggcgt atgagtctgc
    35281 ccccgatcat aaaatctgtg tttcctacgg cgcgtgcggt gtcggcggcg gtattttcca
    35341 cgatctctac agcgtctggg gcggcagcga caccattgtc cccattgatg tttggatccc
    35401 cggctgcccg ccaacaccgg ccgccaccat tcacggtttc gccgtggcgc tcggtttgct
    35461 gcaacagaag attcacgcgg tggattatcg cgatcccacc ggggtgacta tgcaaccgtt
    35521 gtggccgcag atcccgccat cacagcgtat cgccattgag cgagaagcgc ggcggctggc
    35581 gggctatcgt caggggcgag aaatttgcga tcggctcctg cgccatttaa gcgacgatcc
    35641 tacaggaaat cgggttaaca cctggttgcg cgatgccgac gatccacgtc tcaatagtat
    35701 cgttcagcaa ctctttcgcg tactccgggg gttacatgac tgaagagtgc ggggaaattg
    35761 ttttctggac gctgcgaaaa aagtttgtcg ccagtagcga cgagatgccg gaacacagct
    35821 ctcaggtaat gtattactcg ctggctatcg gtcatcacgt tggcgtgatt gattgtctga
    35881 atgtcgcctt ccgctgccca ctgacggaat acgaagattg gcttgcactg gtcgaagagg
    35941 agcaagcccg acgtaagatg ctgggggtga tgacttttgg tgagattgtt attgacgcca
    36001 gccacaccgc cctgttgacc cgggcattcg cgccactggc agatgacgcg acgtctgtgt
    36061 ggcaagcgcg tagcattcaa ttcattcatc tgttggatga aattgtgctg gaaccggcta
    36121 tctatctgat ggccagaaaa attgcgtgag aaggatttct cattaataag gactgttgat
    36181 ggctatgtca gacgaagcga tgtttgcccc gccgcaagga ataacaattg aagcggtaaa
    36241 cggaatgctc gcggagcggt tagcgcagaa acacggcaag gcgtctttat tacgcgcctt
    36301 catcccgctg ccgccgccgt tcagcccggt acaacttatt gaactgcatg ttctcaaaag
    36361 caacttctat taccgctacc atgatgatgg cagcgatgtg acggcaacaa cagagtatca
    36421 gggcgagatg gtcgattatt cgcgttacgc cgtccttctc ggcagtagtg gaatggcgga
    36481 gctacgcttt attcgtaccc acggcagccg ttttactccc caggattgca cactgtttaa
    36541 ctggcaggca cgcattatca ccccggttct gcaatcatgg ctcaatgatg aagcacagca
    36601 ggtggcgctg cgtttgctgg agaaagatcg cgatcatcat cgggtactgg tggatatcac
    36661 taatgcagtg ctgtcacatc ttgatctcga cgatctgatc gctgacgtcg ctcgtgagat
    36721 ccatcatttt ttcggtctgt cttcagtcag tatggtactg ggcgatcatc gaaagaacga
    36781 gaagttcagc ctgtggtgca gcgatctttc tgcctcacat tgtgcgtgtc tgccacacaa
    36841 tatgcctggc gacagtgtat tgctgacaca aacgctacaa acccgacaac cgaccttgac
    36901 gcaccgtgca gatgatctgt ttctctggca acgcgacccg ttattactct tacttgcatc
    36961 taacggctgc gaatctgcgc tccttatacc gcttaccttt ggcaaccata caccgggtgc
    37021 attgttgctg gcgcatacct cttccactct ctttagtgag gaaaactgcc agctactaca
    37081 acacatagcc gatcgcatcg ctattgccgt tggcaatgcc gatgcctgcc gtagaatgac
    37141 cgatttgcag gaaagtttgc agcaagaaaa ccaccagctt agcgagcagc tcctttcgaa
    37201 tctgggcgtc ggtgacatta tctatcaaag ccaggcaatg gaagacctgc tccagcaggt
    37261 agatattgtg gcgaagagcg acagtacggt gttgatttgc ggtgaaaccg gaaccggcaa
    37321 agaggtgatc gccagagcga tccatcaact tagcccgcga cacgacaagc cgctggtcaa
    37381 aatcaactgc gctgccatcc ccgccagtct tctggaaagt gagttattcg gtcatgacaa
    37441 aggggcgttt actggtgcga ttaataccca tcgtggtcgt tttgaaattg ccgatggcgg
    37501 cacgttgttt ctcgatgaaa ttggcgatct gccgttagaa cttcagccta aactgctgcg
    37561 cgtattgcag gaacgggaga ttgagcgtct cggcgggagt agaacgatcc cggtaaatgt
    37621 cagagtcatt gccgccacca accgtgattt gtggcaaatg gttgaagatc gccagtttcg
    37681 cagcgatctc ttttatcgcc tgaatgtctt cccactggaa ttgcctccgc tgcgcgaccg
    37741 tccggaagat atccctcttt tagcaaagca tttcacgcaa aaaatggcgc gccatatgaa
    37801 tcgttcaatt gacgccatcc cgaccgaggc gctacgccag ttgatgtcgt gggactggcc
    37861 gggcaacgtg cgcgagctgg aaaacgtgat tgagcgagcg gtactgctga ctcgcggtaa
    37921 cagtctgaat ttacatctta atgtccgaca aagccgttta ctgccgacgc taaatgaaga
    37981 ttcagcgctt cgcagttcaa tggcgcagtt gctgcacccg acgacgccag agaatgacga
    38041 agaagaacgt cagcgcattg ttcaggtatt gcgagaaacc aatggcattg ttgccgggcc
    38101 ccgtggcgcg gcgacacgat tagggatgaa gcgcaccacg ctgctgtcac gaatgcagcg
    38161 tctgggaatc tcggttcgcg aggtgttgta atctgctttt gcaggagtat gcatgagaaa
    38221 caaactctct ttcgacttgc agttgagcgc cagaaaagcg gcaatcgctg aacggattgc
    38281 cgcccataaa attgcccgca gtaaagtgtc ggtcttttta atggcgatgt ccgctggcgt
    38341 gtttatggcg atcggattta ctttttacct ttccgttatc gccgatgccc cgtcttcata
    38401 ggcattaacc catctggtgg gcggcctttg ctttacactt ggctttattt tgctggcggt
    38461 ttgcggcacc agcctgttca cctcgtcggt aatgacggta atggcaaaaa gtcggggcgt
    38521 tattagttgg cgaacttggc tgattaacgc acttctggtg gcctgcggta atctggcagg
    38581 tattgcctgt ttcagtttgt taatctggtt ttccgggctg gtgatgagtg aaaacgcgat
    38641 gtggggagtc gcgattttac actgcgccga gggcaaaatg catcatacat ttactgaatc
    38701 tgtcagcctc ggcattatgt gcaatctgat ggtttgcctg gcgctgtgga tgagttattg
    38761 cggacgttcg ttatgcgaca aaatcgtcgc catgattttg tccatcaccc tgtttgtcgc
    38821 cagtggcttt gagcactgta tcgccaattt gtttgtgatt ccgttcgcca ttgccattcg
    38881 ccatttcgcc cctacctcct tctggcaact ggcgcacagt agcgcagaca attttccggc
    38941 actgacggtc agccatttta ttaccgccaa tctgctcccg gtgatgctgg gtaatattat
    39001 cggcggtgca gtgctggtga gtatttgtta tcgggctatt tatttacgtc aggaatcctg
    39061 aaaaatcagc ccggcgaaac agttcgtcgg gctgaagatt attcttgcgc gatttgccca
    39121 tcgggccagg cgtggatcac ggctttgatc agcgtcgcca atggaatggc gaagaatacg
    39181 ccccagaatc cccacaaacc accgaagatc accaccgata aaataatcac cagcggatgc
    39241 aggttaaccg cttcggagaa caacaccggt actagcaggt tgccgtccag cgcctgaata
    39301 atcagataca ccgcgaagca gctccagaat tcagtacctg cgccaaactg gaatagcgcc
    39361 acgccaacca ccggaatggt caccacaaat gcgccaatgt acggaataag aaccgagaag
    39421 ccgaccagca ctgccagcag cagcgaatag ttcagcccga agagcaagaa ccccagccag
    39481 gtggcgatcc ccaccacgat catctccagc actttgccgc ggatatagtt ggtgatttgt
    39541 tgattcatct ccttccacac ctgtcctgcc agcccacggt tacgcggcag cacccggcga
    39601 acggcgttca gcatctgctc tttgtctttc agcaggaaga agaccatcaa tggcaccagc
    39661 accagataga cggctatggt cagcaaaccg accagcgagg cgagggaaat tttcaccacc
    39721 gaatcgccca tggtcagcat ccgactgcgc atattttcgg ccattgcatc aataatgccc
    39781 gcgtccatta acaccggata gcggcgcggc aacgtggcgg caaagtcaga aagcttattg
    39841 agcatccccg gcatatcgcg gattaagtag atgccctgtt gccaggcgat aggcagtacc
    39901 acgaacgcca tcagtagcaa tataccgaca aaaaccacca atacaatcga cgtcgcccag
    39961 cggcgggagc agccaataga ttgcaggcgc acggttggcc attccagcaa ataagccagc
    40021 acaatagcca ccagcaacgg agcaagcagg ccactaaaga aaaagataat gccaaatccg
    40081 gcaactaaaa taaccagcaa ggcaatcgct tccgggtcgc taaaacggcg gcgataccat
    40141 tgcatcaaca tttcgagcat aaagaccttc cctgaacctc aagagcggga ttgcgatccg
    40201 caattgtatc gaaatgtcac aaaaaagact tcgcttttta tgacggattc aggaaactaa
    40261 aaagtcattt gagtgggcta atcttcgccg ttacactcaa aggcggcgag gtgggaacga
    40321 tatttcacag tatcggtcaa atgactatct tcagaaatac aggatagagg ttatgttcag
    40381 gcagttgaaa aaaaacctgg ttgcaaccct cattgctgct atgaccattg gtcaggtagc
    40441 cccggcgttt gccgacagcg cagacacctt gccggatatg ggaacctccg caggaagcac
    40501 gctttccatt ggtcaggaaa tgcagatggg cgactattat gtccgccagc tacgcggcag
    40561 cgcgccgtta attaatgacc cgctgttaac gcaatatatt aattcgctgg ggatgcgtct
    40621 ggtttcgcat gccaattcgg ttaagacacc gtttcatttt tttctgatca acaacgacga
    40681 aattaacgcc tttgctttct ttggcggcaa cgtggtgctg cactctgccc tgttccgtta
    40741 ttccgataac gaaagtcaac tggcttcagt tatggcgcac gaaatctccc acgtcaccca
    40801 acgtcacctg gcgcgagcga tggaagatca acaacgcagc gcgccgctga cctgggtcgg
    40861 cgcgttaggt tctattttac tggcgatggc cagtccgcag gcggggatgg cggcgctgac
    40921 cggtacactg gcgggaacgc gccaggggat gatcagtttc acccagcaaa atgaacagga
    40981 agcggaccgc attggtattc aggtgctgca acgctcggga ttcgatccac aggcgatgcc
    41041 gaccttcctg gaaaaattgc tcgatcaggc gcgttactcc tcgcgcccgc cggaaatttt
    41101 attgactcac ccgttgccgg aaagtcgtct ggcagatgcc cgcaaccgtg ctaatcagat
    41161 gcgcccgatg gtggtgcagt cgtcggaaga tttctatctg gcgaaagcgc gcacactggg
    41221 gatgtataat tccggacgta accagctcac cagtgatttg ctggatgaat gggcgaaagg
    41281 aaacgttcgt cagcaacgag cggcgcaata tggtcgtgct ttacaggcga tggaagccaa
    41341 taaatacgac gaggcgcgta aaacgctgca accgttactg gcggcagaac ctggcaacgc
    41401 atggtatctc gatctggcta ccgatatcga tcttgggcaa aacaaagcca atgaggcaat
    41461 caatcgcctg aaaaatgccc gcgatttacg caccaatccg gtgttgcagc tcaacctggc
    41521 gaacgcttat ctgcaaggcg gtcaaccaca agaagcggcc aatattctga atcgctacac
    41581 ctttaataat aaagatgaca gcaacggctg ggatttgctg gcacaggcgg aagccgcgct
    41641 aaataaccgc gatcaggagc tggctgcgcg agcagaaggt tatgcgctcg ccggacgact
    41701 cgatcaggcc atttcgctgt tgagtagcgc cagttcgcag gtaaaattag gcagcctgca
    41761 acaagcgcgt tacgatgcgc gcatcgacca gttgcgccag ctgcaggaac gctttaagcc
    41821 ttataccaag atgtaattcg cattaaagga agaacaatga ccaaacaggt aaaaatctac
    41881 cataacccac gctgttcaaa gagccgggaa acgctgaacc tgctgaaaga aaacggcgtg
    41941 gagccagaag tggtgctcta ccttgagaca cccgccgatg cggcaacgct gcgcgatttg
    42001 ctgaaaatgc tggggatgaa tagcgcccgt gaattgatgc gccagaaaga ggatctttat
    42061 aaagaactaa acctggctga cagttcgctt agcgaagagg cgctgattca ggcaatggtg
    42121 gataatccaa agctgatgga gcgcccgatt atggtcgcga atggcaaagc gcggattggt
    42181 cgtccaccgg agcaggtact ggaaatcgtg ggttaagatg tgctccatcg ctagttgaag
    42241 cacattgccg gatgcgacgc ttgccacgtc ttatccggcc tacgaatact atggattaac
    42301 ttcgcgtagg tcaggcgttc gcgccgcatc cgacaataaa caccttatct acaacttcag
    42361 aatttctttc acaaacggaa tggtcagctt acgttgcgcg gtaatcgacg cacgatccaa
    42421 ctgatccaac gtcataaata gcgtgcgcat ttctctgtcg agccgcttca gcaagaaacg
    42481 ccccacatct tccggcagtt caaaaccacg caaacgcgcg cgtaactgta gcgcctgcaa
    42541 cttatcttca tcagaaagtg gctgcaattt gtagatctgc ccccagtcga gacgcgacgc
    42601 gagatccggt aatcccagat tcaactgccg cggtggacga tcgccggtga tcaacaaccg
    42661 tgttttgccc gattccagaa ttcgattgta gagatcgaaa atcgccatct cccacaactc
    42721 atcgcctgca atacactcaa tgttgtcgat acagaccagc gacaaatgct ccataccgtc
    42781 gagcacttcc ggaacaaacc aggtgcgttt atccagcggg acatagccca ccgcatcgcc
    42841 acgctgcgac aattccgcgc aagccgcgtg cagcagatgg ctgcgccccg cgccttcgcg
    42901 tgcccagaga tagatgtaac cgctatgttc ctgacgcagc acgttttgca gcgcggccag
    42961 taaagaggag ttatcccccg gccagaaact tgcaaaggtt tcgtcgtcag gaagataaag
    43021 tggcaaagag agctgtgccg gtgtgttcag agatacctca accaggattt cacaaaatcg
    43081 cgagaagttt accacagaat cccatgatgt ttgaaccggg cagcaacact gcccggtcgg
    43141 tacattattt gtctgttata tccgcgtctt ctgcgtccag caccacttct tcagggcgca
    43201 gcacactgat cagtttgaaa atcaaactta agccgatacc gacgatggtc gccagcgcca
    43261 taccttttaa ctccgccgcg ccgatgttta ccttcgcgcc actgacgccg atgatcaaaa
    43321 tcacggaagt caggatcagg ttctgtgctt tgttgtaatc cactttcgat tcgatcaaaa
    43381 cacgaatacc ggaagcacca atgacaccat aaagcagcag cgaaacgccg cccatcaccg
    43441 gcaatgggat catctggata gcggcagcca gtttaccgac gcaggaaagc aggatagcga
    43501 aaatcgccgc cccgccgata acccaggtac tgtaaacacg ggtgatcgcc atcacgccaa
    43561 tgttttctcc gtaagtggta tttggcgtag agccaaagaa gccggagatc acggtcgaca
    43621 ggccgttagc aaacatcgaa cggtgcagac ctggatcgcg cagcagatct tttttgacga
    43681 tattagccgt tactaccagg tgccctacgt gttcggcaat aaccactaac gccgctggca
    43741 gaatagtcag aatggcaaac cactcgaagc gcggcgtata gagggttggc agtgcaaacc
    43801 agtgagcatt aataatcggc gtggtatcga caattcccat tgcgaaagag agcgcgtacc
    43861 ccaccagcac gccaattaaa atcgggataa ttgccaggaa accacgaaac agcacggaac
    43921 ctaaaaccgt gaccgccagg gtggtaatag agatgatgat ggttttggag tttggcgttt
    43981 gcccttcagc cgggagtaaa cccgccatac cggcagctac gcccgccagc tccagaccga
    44041 tgacggcaac gattgcgccc attgccgcag gtggaaacag cacgtccaac cagccggtcc
    44101 ccgctttctt cacgataaaa gaaaccaggc agaacagcac gccgcacata ataaagccgc
    44161 ccagcgcgac ttcataccct aacggcaaca gtaacaatac cggtgaaata aaggcaaagc
    44221 tggaaccaag ataagccgga attttccctt tacagatgaa gagatacagc agcgttccaa
    44281 taccgttaaa taacagtaca gtcgccgggt taatatgaaa taagacgggc accaggacgg
    44341 ttgcaccaaa catggcgaac aaatgttgca aactaagcgg gattgtctgt aaaagtggcg
    44401 gtctttcact caccccgata gcacggcgcg tcatagtatt atcctctgta ttatgtgtta
    44461 taggcgcttt actcaaaaaa aagccgactc ttaaagtcgg ctttaattat ttttattctt
    44521 tatttcgtac caaagatttt gtcaccggca tcgccgaggc ccggaataat gtatccgtgc
    44581 tcgttcagtc cctggtcaat cgatgcagta tacagttcga cgtccgggtg cgctttttcc
    44641 agcgcagcga taccttctgg cgcagcgacc agcaccagaa ctttgatgct gctgcagccc
    44701 gcttttttca gcaggtcgat ggtcgcgata acggaaccac cggttgccag cattgggtca
    44761 acgatcagcg ccatacgctc atcgatgtta gaaaccagtt tctggaagta cggtaccggc
    44821 tccagcgttt cttcattacg gtacataccg acaacgctga tgcgcgcgct cggaacgttt
    44881 tccagcacac cgtccatcat accaagaccc gcacgcagaa ttggcacaac ggtaattttc
    44941 ttacctttga tctggtcgat ttctaccggg ccgttccagc cttcgatagt tactttttcc
    45001 gtttcgaggt cggcggtcgc ttcgtaagtc agcaggctac ccacttcgga agcgagttcg
    45061 cgaaagcgct tggtgctgat atcttgctca cgcatcagtc ccagcttgtg tttgacgagt
    45121 gggtgtttga cttccacgat cttcatactc tttctccttt gaggggcagc cacaaaaaaa
    45181 atcgacggat tatacctcct ttcttcaagg cggcaatatt attttcgttg actttagtca
    45241 aaatgataac agtttgagat aaagttattt tatattcaaa tggttatgaa agaagattat
    45301 tccatccgaa aactaacctt tgccctggca caagtcttct ttcgccgcgc gcccggggaa
    45361 aagacgtgca aaaaggttgt gtaaaacagt ctcgcaaacg tttgctttcc ctgttagaat
    45421 tgcgccgaat tttatttttc taccgcaagt aacgcgtggg gacccaagca gtgaccgata
    45481 aaacctctct tagctacaaa gatgccggtg ttgatattga cgcgggtaat gctctggttg
    45541 gaagaatcaa aggcgtagtg aagaaaacgc gtcgtccgga agtgatgggc ggtctgggcg
    45601 gcttcggtgc gctgtgtgca ttgccgcaaa aatatcgtga acccgtgctg gtttccggca
    45661 ctgacggcgt aggtaccaag ctgcgtctgg caatggactt aaaacgtcac gacaccattg
    45721 gtattgatct ggtcgccatg tgcgttaatg acctggtggt gcaaggtgcg gaaccgctgt
    45781 ttttcctcga ctattacgca accggaaaac tggatgttga taccgcttca gcggtgatca
    45841 gcggcatcgc ggaaggttgt ctgcaatcgg gctgttctct ggtgggtggc gaaacggcag
    45901 aaatgccggg gatgtatcac ggtgaagatt acgatgtcgc gggtttctgc gtgggcgtgg
    45961 tagaaaaatc agaaatcatc gacggctcta aagtcagcga cggcgatgtg ctgattgcac
    46021 tcggttccag cggtccgcac tcgaacggct attcgctggt gcgcaaaatt cttgaagtca
    46081 gcggttgtga tccgcaaacc accgaacttg atggtaagcc attagccgat catctgctgg
    46141 caccgacccg catttacgtg aagtcagtgc tggagttgat tgaaaaggtc gatgtgcatg
    46201 ccattgcgca cctgaccggc ggcggcttct gggaaaacat tccgcgcgta ttgccagata
    46261 atacccaggc agtgattgat gaatcttcct ggcagtggcc ggaagtgttc aactggctgc
    46321 aaacggcagg taacgttgag cgccatgaaa tgtatcgcac cttcaactgc ggcgtcggga
    46381 tgattatcgc cctgcctgct ccggaagtgg acaaagccct cgccctgcta aatgccaacg
    46441 gtgaaaacgc gtggaaaatc ggtatcatca aagcctctga ttccgaacaa cgcgtggtta
    46501 tcgaataatg aatattgtgg tgcttatttc cggcaacgga agtaatttac aggcaattat
    46561 tgacgcctgt aaaaccaaca aaattaaagg caccgtacag gcagttttca gcaataaggc
    46621 cgacgcgttc ggccttgaac gcgcccgcca ggcgggtatt gcaacgcata cgctcatcgc
    46681 cagcgcgttt gacagtcgtg aagcctatga ccgggagttg attcatgaaa tcgacatgta
    46741 cgcacccgat gtggtcgtgc tggctggttt tatgcgcatt ctcagcccgg cgtttgtctc
    46801 ccactatgcc gggcgtttgc tgaacattca cccttctctg ctgccgaaat atcccggatt
    46861 acacacccat cgtcaggcgc tggaaaatgg cgatgaagag cacggtacat cggtgcattt
    46921 cgtcaccgat gaactggacg gtggcccggt tattttacag gcgaaagtcc cggtatttgc
    46981 tggtgatacg gaagatgaca tcaccgcccg cgtgcaaacc caggaacacg ctatttatcc
    47041 actggtgatt agctggtttg ccgatggtcg tctgaaaatg cacgaaaacg ccgcgtggct
    47101 ggatggtcaa cgtctgccgc cgcagggcta cgctgccgac gagtaatgcc cccgtagtta
    47161 aagcgccagc tctgccgctg gcgtttttca attcacctgt aaatcgcaag ctccagcagt
    47221 ttttttcccc cttttctggc atagttggac atctgccaat attgctcgcc ataatatcca
    47281 ggcagtgtcc cgtgaataaa acggagtaaa agtggtaatg ggtcaggaaa agctatacat
    47341 cgaaaaagag ctcagttggt tatcgttcaa tgaacgcgtg cttcaggaag cggcggacaa
    47401 atctaacccg ctgattgaaa ggatgcgttt cctggggatc tattccaata accttgatga
    47461 gttctataaa gtccgcttcg ctgaactgaa gcgacgcatc attattagcg aagaacaagg
    47521 ctccaacgct cattcccgcc atttactggg caaaattcag tcccgggtgc tgaaagccga
    47581 tcaggaattc gacggcctct acaacgagct attgctggag atggcgcgca accagatctt
    47641 cctgattaat gaacgccagc tctccgtcaa tcaacaaaac tggctgcgtc attattttaa
    47701 gcagtatctg cgtcagcaca ttacgccgat tttaatcaat cctgacactg acttagtgca
    47761 gttcctgaaa gatgattaca cctatctggc tgtggaaatt atccgtagcg atagcatccg
    47821 ttacgcgctg ctggagatcc catcagataa agtgccgcgc tttgtgaatt taccgccaga
    47881 agcgccgcgt cgacgcaagc cgatgattct tctggataac attctgcgtt actgccttga
    47941 tgatattttc aaaggcttct ttgattatga cgcgctgaat gcctattcaa tgaagatgac
    48001 ccgcgatgcc gaatacgatt tagtgcatga gatggaagcc agcctgatgg agttgatgtc
    48061 ttccagtctc aagcagcgtt taactgctga gccggtgcgt tttgtttatc agcgcgatat
    48121 gcccaatgcg ctggttgaag tgttacgcga aaaactgact atttcccgct acgactccat
    48181 cgtccccggc ggtcgttatc ataattttaa agactttatt aatttcccca atgtcggcaa
    48241 agccaatctg gtgaacaaac cactgccgcg tttacgccat atttggtttg ataaagccca
    48301 gttccgcaat ggttttgatg ccattcgcga acgcgatgtg ttgctctatt atccttatca
    48361 cacctttgag catgtgctgg aactgctgcg tcaggcttcg ttcgacccga gcgtactggc
    48421 gattaaaatt aacatttacc gcgtggcgaa agattcacgc atcatcgact cgatgatcca
    48481 cgccgcacat aacggtaaga aagtcactgt ggtggttgag ttacaggcgc gtttcgacga
    48541 agaagccaac attcactggg cgaagcgcct gaccgaagca ggcgtgcacg ttatcttctc
    48601 tgcgccgggg ctgaaaattc acgccaaact gttcctgatt tcacgtaaag aaaacggtga
    48661 agtggtccgt tacgcacaca tcgggaccgg gaactttaac gaaaaaaccg cgcgtcttta
    48721 tactgactat tcgttgctga ccgcagatgc gcgtatcacc aacgaagtac ggcgggtatt
    48781 taactttatt gaaaacccat accgcccggt gacatttgat tatttaatgg tgtcaccgca
    48841 aaactctcgc cgtctgttat atgaaatggt agaccgcgaa atcgccaacg cgcagcaagg
    48901 gctgcccagt ggtatcaccc tgaagctaaa taaccttgtc gataaaggcc tggttgatcg
    48961 tctgtatgcg gcctccagct ccggcgtacc ggttaatctg ctggttcgcg gaatgtgttc
    49021 gctgatcccc aatctggaag gcattagcga caacattcgt gccatcagta ttgttgaccg
    49081 ttaccttgaa catgaccggg tttatatttt tgaaaatggc ggcgataaaa aggtctacct
    49141 ttcttccgcc gactggatga cgcgcaatat tgattatcgt attgaagtgg cgacaccgct
    49201 gctcgatccg cgcctgaagc agcgggtgct ggacatcatc gacatattgt tcagcgatac
    49261 ggtcaaagca cgttatatcg ataaagaact cagtaatcgc tacgttcccc gcggcaatcg
    49321 ccgcaaagta cgggcgcagt tggcgattta cgactacatc aaatcactcg aacaacctga
    49381 ataaccctat gccaatacac gataaatccc ctcgtccgca ggagtttgct gcggtcgatc
    49441 ttggttcaaa cagttttcac atggtcatag cccgtgtggt agatggtgcc atgcagatta
    49501 ttggccgcct gaaacagcgg gtgcatctgg cggacggcct ggggccagat aatatgttga
    49561 gtgaagaggc aatgacgcgc ggtttgaact gtctgtcgct gtttgccgaa cggctacaag
    49621 ggttttctcc tgccagcgtc tgtatagtcg gcacccatac gctgcgtcag gcgctgaacg
    49681 ccactgactt tctgaaacgc gcggaaaagg tcattcccta cccgattgaa attatttccg
    49741 gtaatgaaga agcccgtctg atttttatgg gcgtggaaca tacccaaccg gaaaaaggtc
    49801 gcaaactggt tattgatatt ggcggcggat ctacggaact ggtgattggt gaaaatttcg
    49861 aacctattct cgttgaaagc cgccggatgg gttgtgtcag ctttgcccag ctttattttc
    49921 ctggcggggt catcaataaa gagaattttc agcgcgctcg catggcggca gcacaaaaac
    49981 tggaaacttt aacctggcaa ttccgtattc agggctggaa cgttgcaatg ggcgcttccg
    50041 gtaccataaa agccgcccat gaagtgttaa tggaaatggg cgagaaagac gggataatta
    50101 ccccggaacg tctggaaaaa ctggtaaaag aaattttacg tcaccgtaat ttcgcatcgc
    50161 tgagtttacc gggtctttcc gaagagcgga aaacagtctt cgttcctgga ctggcgattt
    50221 tatgtggtgt gtttgatgct ttagccatcc gtgaactgcg cctttctgac ggggcgttac
    50281 gcgaaggcgt actgtatgaa atggaaggac gtttccgtca tcaggatgtg cgtagtcgca
    50341 ccgccagcag cctcgccaac cagtatcaca tagacagcga acaggcccga cgagtgctgg
    50401 ataccactat gcaaatgtac gaacagtggc gggaacagca accgaagctg gcgcatccgc
    50461 aactggaggc gctactgcga tgggccgcca tgctgcatga ggtcgggttg aatatcaacc
    50521 acagcggttt gcatcgccac tccgcttata ttctgcaaaa cagtgacttg ccgggtttta
    50581 atcaggaaca gcagctgatg atggcgacac tggtgcgcta tcaccgtaaa gcgattaagc
    50641 tcgacgatct gccgcgcttt accttgttta agaagaaaca gttcctgcca ctgatacagc
    50701 tattgcgcct tggcgtatta ctaaacaatc aacgtcaggc aaccaccaca ccgccaacat
    50761 tgacactgat tactgatgac agtcactgga cactgcgttt cccgcatgac tggtttagtc
    50821 agaatgcgct ggtactgctt gatctggaaa aggagcaaga atactgggaa ggcgtggctg
    50881 gctggcggtt gaaaattgaa gaagaaagta ccccagaaat cgccgcttaa tgctttgcgg
    50941 gccgacgaga aatgtcggcc cgcattattc aggcactttc gcgaatgggt tcgatttcat
    51001 tcagcgtatc aattaacggt tgcggcttac caataagata accctgcata taatcgatcc
    51061 ccaaagagag caccgcctcg cggatctctt cgttttcaac gtactctgcc actacccgca
    51121 ttttcttcat tcgcgccaga tggcaaatcg acgccacaat ctgataatcc agactatttg
    51181 acacaatatt gcggataaag ctgccgtcaa ttttaagcag atcggcattc acatttttaa
    51241 gccgtgcata gctggcgtag ccggtgccga aatcatcaat cgcaatctgg cagcctaatt
    51301 cctgaagatg ctgcaaggtg atttgcgcct gcttaacatt ggtcagagca ttactttcgg
    51361 tgacttcaaa aataagttgc cacgcttcaa tctgatattt agccagcaac tgactgactt
    51421 caacaggaaa acgagcctga cataccgagg ttggagacag attaatagca aaacggtgag
    51481 cgggcatctt cgctctgttt tcagccataa attgcagcgt atgctcaatg acccacatgt
    51541 cgatactcga cgataaacca aattcgtgcg cgaccggcaa gaagctaccg gggccgatca
    51601 gttcatcatt ctcacctttc atgcgcagaa gaatttcatg gtaaacatca ccacgcatac
    51661 cggtaatcgg ctgggccatc aggaaaaaat ggttgtgttc cagcgcctgc tgtagtcgat
    51721 tcatcatcgc gactttatct ttcaattcgc gttgcaaata cattgccccg caacgctgca
    51781 tattttccgg ggcgttggtc acgatggaaa gttcggcgac cgtatttagc tctcccagca
    51841 gcaggtagat atgattcact ggcgagcgca catagcagta actgacgcca atctgcggtt
    51901 gcatcggcat cccatcccaa aagaaacgaa attgcttgag atgactatcc agtgcggtaa
    51961 tgcgctcctg gtgcgattct gtattcagtc gcagcgcgag atcgttaccc gaaagctgat
    52021 aaacatcttc acccggttcc agcaagggtg acagccagtg agaaagtttt tgcttgta
//
LOCUS       DAEEUE010000019        49769 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.019, whole genome shotgun sequence.
ACCESSION   DAEEUE010000019 DAEEUE010000000
VERSION     DAEEUE010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49769
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.019"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..219
                     /locus_tag="KLK84_001174"
     CDS             <1..219
                     /locus_tag="KLK84_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE2008580.1"
                     /translation="SRKGNCLDNAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIE
                     YYNSRRISLKLKGLTPIEYRNQTYMPRV"
     gene            321..473
                     /gene="hokA"
                     /locus_tag="KLK84_001175"
     CDS             321..473
                     /gene="hokA"
                     /locus_tag="KLK84_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HBE2008581.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(660..872)
                     /gene="cspA"
                     /locus_tag="KLK84_001176"
     CDS             complement(660..872)
                     /gene="cspA"
                     /locus_tag="KLK84_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HBE2008582.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1153..1443)
                     /locus_tag="KLK84_001177"
                     /pseudo
     CDS             complement(1153..1443)
                     /locus_tag="KLK84_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            1877..2587
                     /locus_tag="KLK84_001178"
     CDS             1877..2587
                     /locus_tag="KLK84_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HBE2008583.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2637..3611)
                     /gene="ghrB"
                     /locus_tag="KLK84_001179"
     CDS             complement(2637..3611)
                     /gene="ghrB"
                     /locus_tag="KLK84_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HBE2008584.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3715..4365)
                     /locus_tag="KLK84_001180"
     CDS             complement(3715..4365)
                     /locus_tag="KLK84_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HBE2008585.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            4528..6861
                     /gene="bisC"
                     /locus_tag="KLK84_001181"
     CDS             4528..6861
                     /gene="bisC"
                     /locus_tag="KLK84_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HBE2008586.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6830..7270)
                     /locus_tag="KLK84_001182"
     CDS             complement(6830..7270)
                     /locus_tag="KLK84_001182"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBE2008587.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7267..7830)
                     /gene="tag"
                     /locus_tag="KLK84_001183"
     CDS             complement(7267..7830)
                     /gene="tag"
                     /locus_tag="KLK84_001183"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBE2008588.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            7988..8686
                     /locus_tag="KLK84_001184"
     CDS             7988..8686
                     /locus_tag="KLK84_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBE2008589.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8915..10123
                     /locus_tag="KLK84_001185"
     CDS             8915..10123
                     /locus_tag="KLK84_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE2008590.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10447..12138
                     /gene="eptB"
                     /locus_tag="KLK84_001186"
     CDS             10447..12138
                     /gene="eptB"
                     /locus_tag="KLK84_001186"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HBE2008591.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12230..12306
                     /locus_tag="KLK84_001187"
     tRNA            12230..12306
                     /locus_tag="KLK84_001187"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12264..12266,aa:Pro,seq:cgg)
     gene            13050..14657
                     /gene="dppA"
                     /locus_tag="KLK84_001188"
     CDS             13050..14657
                     /gene="dppA"
                     /locus_tag="KLK84_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HBE2008592.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14965..15984
                     /gene="dppB"
                     /locus_tag="KLK84_001189"
     CDS             14965..15984
                     /gene="dppB"
                     /locus_tag="KLK84_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HBE2008593.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15994..16896
                     /gene="dppC"
                     /locus_tag="KLK84_001190"
     CDS             15994..16896
                     /gene="dppC"
                     /locus_tag="KLK84_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HBE2008594.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16907..17890
                     /gene="dppD"
                     /locus_tag="KLK84_001191"
     CDS             16907..17890
                     /gene="dppD"
                     /locus_tag="KLK84_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBE2008595.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17887..18891
                     /gene="dppF"
                     /locus_tag="KLK84_001192"
     CDS             17887..18891
                     /gene="dppF"
                     /locus_tag="KLK84_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HBE2008596.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18921..20192)
                     /locus_tag="KLK84_001193"
     CDS             complement(18921..20192)
                     /locus_tag="KLK84_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBE2008597.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20668..20775
                     /locus_tag="KLK84_001194"
     CDS             20668..20775
                     /locus_tag="KLK84_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBE2008598.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            21151..21258
                     /gene="ldrD"
                     /locus_tag="KLK84_001195"
     CDS             21151..21258
                     /gene="ldrD"
                     /locus_tag="KLK84_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HBE2008599.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            21634..21741
                     /locus_tag="KLK84_001196"
     CDS             21634..21741
                     /locus_tag="KLK84_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBE2008600.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            22117..22224
                     /locus_tag="KLK84_001197"
     CDS             22117..22224
                     /locus_tag="KLK84_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBE2008601.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(22311..23990)
                     /gene="bcsG"
                     /locus_tag="KLK84_001198"
     CDS             complement(22311..23990)
                     /gene="bcsG"
                     /locus_tag="KLK84_001198"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HBE2008602.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(23987..24178)
                     /gene="bcsF"
                     /locus_tag="KLK84_001199"
     CDS             complement(23987..24178)
                     /gene="bcsF"
                     /locus_tag="KLK84_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HBE2008603.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(24175..25746)
                     /gene="bcsE"
                     /locus_tag="KLK84_001200"
     CDS             complement(24175..25746)
                     /gene="bcsE"
                     /locus_tag="KLK84_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HBE2008604.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            26019..26207
                     /locus_tag="KLK84_001201"
     CDS             26019..26207
                     /locus_tag="KLK84_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HBE2008605.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            26219..26971
                     /gene="bcsQ"
                     /locus_tag="KLK84_001202"
     CDS             26219..26971
                     /gene="bcsQ"
                     /locus_tag="KLK84_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HBE2008606.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLCYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            26968..27327
                     /locus_tag="KLK84_001203"
     CDS             26968..27327
                     /locus_tag="KLK84_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HBE2008607.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            27324..29585
                     /gene="bcsA"
                     /locus_tag="KLK84_001204"
                     /pseudo
     CDS             27324..29585
                     /gene="bcsA"
                     /locus_tag="KLK84_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            29596..31935
                     /gene="bcsB"
                     /locus_tag="KLK84_001205"
     CDS             29596..31935
                     /gene="bcsB"
                     /locus_tag="KLK84_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBE2008608.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISSRRLNPDNE"
     gene            31942..33048
                     /gene="bcsZ"
                     /locus_tag="KLK84_001206"
     CDS             31942..33048
                     /gene="bcsZ"
                     /locus_tag="KLK84_001206"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HBE2008609.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            33107..33283
                     /locus_tag="KLK84_001207"
                     /pseudo
     CDS             33107..33283
                     /locus_tag="KLK84_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
     gene            33611..35359
                     /gene="bcsC"
                     /locus_tag="KLK84_001208"
                     /pseudo
     CDS             33611..35359
                     /gene="bcsC"
                     /locus_tag="KLK84_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138097.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            35441..37429
                     /gene="hmsP"
                     /locus_tag="KLK84_001209"
     CDS             35441..37429
                     /gene="hmsP"
                     /locus_tag="KLK84_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HBE2008610.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNFSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            37615..38901
                     /gene="dctA"
                     /locus_tag="KLK84_001210"
     CDS             37615..38901
                     /gene="dctA"
                     /locus_tag="KLK84_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HBE2008611.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            39122..40618
                     /locus_tag="KLK84_001211"
     CDS             39122..40618
                     /locus_tag="KLK84_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBE2008612.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(40714..41643)
                     /gene="kdgK"
                     /locus_tag="KLK84_001212"
     CDS             complement(40714..41643)
                     /gene="kdgK"
                     /locus_tag="KLK84_001212"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HBE2008613.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            41875..42642
                     /gene="pdeH"
                     /locus_tag="KLK84_001213"
     CDS             41875..42642
                     /gene="pdeH"
                     /locus_tag="KLK84_001213"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HBE2008614.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            42712..44772
                     /locus_tag="KLK84_001214"
     CDS             42712..44772
                     /locus_tag="KLK84_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBE2008615.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(44954..46276)
                     /locus_tag="KLK84_001215"
     CDS             complement(44954..46276)
                     /locus_tag="KLK84_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HBE2008616.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(46687..47700)
                     /gene="yhjD"
                     /locus_tag="KLK84_001216"
     CDS             complement(46687..47700)
                     /gene="yhjD"
                     /locus_tag="KLK84_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HBE2008617.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(47749..48648)
                     /locus_tag="KLK84_001217"
     CDS             complement(47749..48648)
                     /locus_tag="KLK84_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE2008618.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            49168..>49769
                     /locus_tag="KLK84_001218"
     CDS             49168..>49769
                     /locus_tag="KLK84_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBE2008619.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT        11929 a        12410 c        13437 g        11993 t
ORIGIN      
        1 tccagaaaag gcaattgtct ggataatgct gtggtggagt gtttctttgg aaccttaaag
       61 tcggagtgtt tttatcttga tgagttcagt aatataagcg aactgaagga tgctgttacg
      121 gaatatattg aatactataa cagcagaaga attagcctga aattaaaagg tctgactcca
      181 attgaatatc ggaatcagac ctatatgcct cgtgtttaac tgtccaactt tttggggtca
      241 gtacaaagtg aggctaccct atgcctgaac accataaggt tagcctctta ctcgttggaa
      301 atcaacacag ggggctggga atgccgcaga aatatagatt actttcttta atagtgattt
      361 gtttcacgct tttatttttc acctggatga taagagattc actgtgtgaa ttgcatatta
      421 aacaggagag ttatgagctg gcggcgtttt tagcctacaa attgaaagag taagagtctt
      481 cggcgggaaa ttattcccgc cttacttacg gcgttgcgca ttctcattgc acccatattt
      541 attcttcaca aaaataataa tagattttat tacgcgatcg attatttatt tcctgaaaaa
      601 aaataaaaaa atccccgcca aatggcaggg atcttagatt ctgtgctttt aagcagagat
      661 tacaggctgg ttacgttacc agctgccggg cctttagcgc cgctttcgat ggtgaaggac
      721 actttctgac cttcgtccag agatttgtaa ccatcgttct ggatagcaga gaagtgtacg
      781 aacacatctt tagagccatc gtcaggagtg atgaagccga agcctttgtc agcgttgaac
      841 cattttacga taccagtcat tttaccggac atagtgtatt acctttaata attaagtgtg
      901 cctttcggcg atatggcgtg ctttacagat tttgaagcgt taaaggaatg tgcactacga
      961 ggggtatcaa cgataactct tgaagggact tgccttacta cactgcttta atggtctgta
     1021 cgtcaaaccg ttgatgtgca ttaagccact cattggcggg tgatgcaaca attatttttc
     1081 atatttatga ttaatcggtt ggaaaacggt cctgtcatca ggaccgtaaa cagcaataaa
     1141 gtggataaaa gtctatttca tcaactgctt acttaatgcc gggttggctt gaatcaaacg
     1201 catcaatttt agttcggcac ttgaaggctt cacgcgtctg gattcccatt cctttaccat
     1261 ggcgactgat acgcccaaaa cccgggcgaa atcatcgatt tttaatcctg tcccttttca
     1321 taactgctca atttcggtac aggacgttgt tctgtgcgtc agggtaattt tctgcgtttc
     1381 atctttaaaa acaatctgtt ccaggctgct caacagctca tgcattggat ctttatattc
     1441 cattgaaaac tcctcaaatc acactgcggg atcgtgaatt gcatcgaagc tcattaagaa
     1501 tagtcgggaa aagcagacac agcggggtga agtcagggca tgctctggct atgattcgat
     1561 tcagcctgtc atttagcgtg acttatagcc acctaacgat ctgataaatg ccgatatatc
     1621 tcttatcact cctgttttgt aaagatttcg caaatctaac cttctgcgaa ggcgtaaatc
     1681 tctggctaca aaaaaggcaa cggcactatc attttttaac aatgaaacaa caaattaacc
     1741 ttgtttcatt ttctccatga ttttaccagg aagattacgt agaaagggta tcctgcaagc
     1801 ctgtcctgga ctatccttgt cacgtcagac acgcgtgtgt cgttgtgcgc tttttttggg
     1861 tgaaaggagt aagaaaatgg cgacaggaaa gtcctgctct cgctggtttg cgcctcttgc
     1921 ggcgttatta atggtagtta gcctgagtgg gtgttttgat aaagaaggcg atcagcgtaa
     1981 agcgtttatc gacttcctgc aaaatacagt gatgcgtagc ggtgaacgtc taccaaccct
     2041 gactgccgat cagaaaaaac agtttggtcc ttttgtctct gattacgcga ttctgtatgg
     2101 ttattctcag caggtgaatc aggcgatgga ttccggtctg cgcccggtcg tagacagcgt
     2161 taacgcaatt cgcgtaccgc aggattacgt tacgcaaagc ggtccgctgc gtgaaatgaa
     2221 tggttcgctg ggtgtgctgg cgcaacaact gcaaaatgcg aagttgcagg ccgatgctgc
     2281 gcactctgcg ctaaaacaga gcgatgacct gaaaccggta tttgatcagg cattcaccaa
     2341 agtggtaacg acgccagctg atgcattgca accgttgatt ccggcggcac aaactttcac
     2401 gcaacaactg gtgatggttg gagactacat tgctcagcaa ggtactcagg tgagctttgt
     2461 cgcaaatggc atccagttcc cgacttcaca gcaggcaagt gaatataaca aactgattgc
     2521 gccattaccg gcacaacatc aggcgtttaa tcaggcctgg actacagcag ttactgcaac
     2581 ccaataaaga gtaaaagccc gagcgattat cgggctttac ttcacgcagt cgcagcttag
     2641 tccgcgacgt gaggattcac acagttcttc tcaacctttc cttgtaacgc atcaatcaaa
     2701 ttatccacgg cacaggcggc catgccataa cgcgtctcat gggtggcaga tccaatatgc
     2761 ggtactgcga cgacgttggc cattgagagc aacggcgaat ctacggacag tggctcttgt
     2821 tcgaagacat ccagcccggc ggcgtgaatt tcgcctttct gcaatgctgc gatcagtgca
     2881 ttttcgtcaa ccaccgggcc acgtccggca ttaatgaaaa tggcggagga tttcattttg
     2941 gcgaattgtt ctgcgccaaa cagatgatgc gtctcatcag ttaacggcag gatcaggcaa
     3001 acgaaatctg actcttgtaa cagagtatcc aaatcgcagt agcgggcgtt gaagcgttct
     3061 tctgcttctt tatggtggcg gcgcgcgtta tagaggatgg gcatgttgaa gccaaagtgc
     3121 acacgttgtg ccagcgccat gccgatccgt cccatcccga caatgcccag tgttttatgg
     3181 tgaacgtcag tgccgtacca gtccgggcct atgctcgcgg tccattcgcc tgcttttacc
     3241 cgttctgcta cctccacaac ccgacgagcg gtagacaaca ccagcgccat cagcgtatcg
     3301 gcgacggttt ctgttaatac ggttggcgtg tgcatcagca gaatttttcg ggcggtaagc
     3361 gcatcgacat caaaattgtc atagccgacg gagatcgttg atgtggcacg cagtttcggc
     3421 attttttcca gcaatgcggc atctacattc tcgtttgaac ccagtaaacc ttcagcttct
     3481 gcaaaaattg ctgcattttg aacgacggtt tgtgggctga ggtttgccac ctggtgaacg
     3541 gtgaaatgcg cttgcaggcg ttgcagtaaa tcatcaggta aggctttgta gaggataacg
     3601 gacggcttca tgcttctctc cattcactga taaggcgatc accttagcct gaaatatgat
     3661 gaaggaaaac tgaagaatgc caggtgattt ctgcatcacc tggcatgaaa gggattacag
     3721 cgggcttaag gtaatttcta cacggcggtt ttgcgcctta ccttctgcgg tgctgttgct
     3781 ggcgattggg ttagccgggc caaggccctg agtacggatg cggctggcgt ccacgccctg
     3841 ggtgatcaac gcgctggcaa cggaatccgc acgctgctgg gagagacgca tgttcaggtc
     3901 gtgacccccc gtgctgtcgg tataaccaat cacgttaacc gccgttttcg gatactcttt
     3961 cagcaccatt gccacgccgg tcagggtgtt agcgcctgcc ggtttcaggg gagcgctgct
     4021 gctgtcgaag gtcacattgt tcggcatatt gaggataatg ttatccccgc tgcgggttac
     4081 gctaacacca gtgccgcgca ttttgtcacg cagcttcgct tcctgcacat ccatgtaata
     4141 acccacgccg ccgcccagag ctgcgcctgc tgctgcgcca atcagcgcgc ctttaccgcg
     4201 atctttcttc gaagaagaga gcgcaccaat acccgcgccc acgagagagc ccagacctgc
     4261 gccgatagca gatttgcctg cttcgcgttc gccggtgtaa gggttagttg tgcagccaga
     4321 taccgccaga gcgccactca ctacggcggc aagtaagata aacacgtttc ttcattgtta
     4381 atccttaata acctttttat tctttgtcac gggttccgtg gcgggagatt atgccgcgtg
     4441 aacatgaaga ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat
     4501 acatctaacc tttcaggagt cttcggtttg gccaactcat cctcacgata ttccgttctg
     4561 actgccgccc actgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg
     4621 cgtggcgcgt tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt
     4681 cacagcaata cgcgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa
     4741 aacccgcaag gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg
     4801 gatcttattc accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt
     4861 gctggttcct acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa
     4921 cgctatatgg cgctggcagg cggttatacc gggcatctgg gggattattc gaccggtgcg
     4981 gcacaggcga tcatgccgta tgtcgtaggt ggcagcgaag tttatcaaca gcaaaccagt
     5041 tggccgctgg tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat
     5101 acgctgaaaa ttgcgtggaa tgcatccgat gagcaggggc tttcttactt ttccgcactg
     5161 cgtgacagtg ggaaaaagct gatctgcatt gatccaatgc gatcggaaac cgtcgatttc
     5221 tttggcgata aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg
     5281 gggatcgccc atacgctggt ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc
     5341 accacaggtt atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa
     5401 aacgccgaat gggcggcaga gatttgtggt gttggcgcag cgaaaatccg cgagctggcg
     5461 gctattttcc accaaaatac caccatgctg atggcaggct ggggaatgca gcgccaacag
     5521 tttggtgagc aaaaacactg gatgatcgtc acgctggcag caatgttggg gcaaatcggc
     5581 acacccggcg gcggttttgg tctttcttac cattttgcca atggtggtaa ccccacgcgg
     5641 cgttctgcgg tgctctcttc catgcagggc agcttgccgg gtggctgcga tgcggtggat
     5701 aaaatccctg ttgcccgcat tgttgaagca ctggaaaacc ctggtggcgc atatcaacac
     5761 aacggtatga accgacattt cccggatatt cgttttatct ggtgggcggg cggtgccaac
     5821 tttactcatc atcaggatac caatcgtctg atccgtgcct ggcaaaaacc ggagctggtg
     5881 gtgatctctg aatgcttctg gacggcggcg gcgaaacacg cggatattgt tctgcctgcg
     5941 actacctctt ttgagcgtaa tgatctcacc atgaccggtg attacagtaa tcagcatctg
     6001 gtgccgatga agcaagtggt gccgccacgc tatgaagcgc gtaatgattt tgatgttttt
     6061 gccgagttga gtgaacgttg ggagaagggc ggctatgcgc ggtttacgga aggaaaaagt
     6121 cagctgcaat ggctggaaac gttttataac gttgcccggc agcgcggggc aagccagcag
     6181 gttgaattgc cgccatttgc tgagttctgg caagccaacc agttaattga gatgccggaa
     6241 aacccggaca gcgagcggtt tattcgcttc gctgattttt gccgcgatcc gctggcgcat
     6301 ccgttaaaaa ccgccagcgg caagattgaa atcttctcac agcgtattgc cgattacggt
     6361 tacccggatt gccctgggca tccaatgtgg ctggagccgg acgaatggca gggcaatgcc
     6421 gaaccagaac agttgcaggt actttctgcc catccggcgc accgcctgca cagccagctg
     6481 aattacagtt ctctgcgcga attgtacgcg gtagcaaacc gtgagcctgt caccattcat
     6541 cctgacgatg ctcaggcgcg cggcataacc gaaggggata tggtgcgggt gtggaactcc
     6601 cgtgggcaga tccttgccgg agcggtcatt agcgagggaa ttaaacctgg cgtgatttgc
     6661 attcacgaag gggcatggcc ggatctggat ttaaccgctg acggtatttg taaaaacggc
     6721 gcagtgaacg tgctgaccaa agatctcccc agctcgcggc tggggaatgg ctgtgcgggt
     6781 aatacgacgc tggcatggct ggaaaaatac aacggtccgg aactgacact tacagcgttt
     6841 gaaccaccgg ccagctcata atccaggtgg gtagttgggt ttcatcctgc catgcgcaat
     6901 cgacaatgtg aaaaccctgc gcacggtaaa aatctatcgc cggttgattt ttttgataaa
     6961 cctccagcat cagatgggga tagcgctgct gcacatactg catcagcgcc ttaccaatac
     7021 cgcgtctgac ggccttcggt gcgacaaaca ttgctgccag aaatcggcct tccataatgc
     7081 tgacaaaacc gagaagctta ccgtcctctt cccagaccca gttttgcgcg ttggcaagat
     7141 aggcatcccg caccagcgga atgcagtcac gccagtaatt cgcttttata aagggatgcc
     7201 cccaggttgt actttccagc cacagttcga ggatcgcggg gagttctgaa cgttgcgctt
     7261 cccgaatcat ggtttatttc ccagatagca acagcagcca accacatgat cattcaccag
     7321 cccacatgcc tgcataaagg agtaacagat tgtggtgccg acaaacttaa aaccacgttt
     7381 tttcagtgcc ttagataggg cgtcggaggc ggacgtagat gtgggaattt cgctcaacgt
     7441 tgtggcttgt gtcacctgtg gctgatgatt tacaaacgac cagacaaagt cgggaaacgg
     7501 ttcgccgttc tgttccattt gcaggtacgc ccgcgcatta ccaataattg cctgaatttt
     7561 ccctcgatgg cggataatcc cggcgtcctg taccagtctt tcgacatcct cttcctgcat
     7621 tgctgcgacc ttcaccggat cgaactgatg aaagcaggcg cgatagtttt cgcgtttttt
     7681 gaggacggtg atccacgata acccagcctg ctgcccttca aagcagatca tttcgaacag
     7741 ttttttactg tcagtttcag gcacgcccca ctcattatca tggtaggcaa tataaagcgg
     7801 gccctgactc acccagccgc aacgttccat actttccctc gctatgtacc cgatcgtaaa
     7861 aatttcagcc gcttttcttg acgcatcggt gaaaaagtca atatttaata cagggctact
     7921 atgcccgata tccttcatac aatgacaata atattgccga attcggctct tccctggagt
     7981 cgtgttaatg atcataaaaa aaagcggtgg tcgctggcag ctaagcctgc tggcgagcgt
     8041 ggtaatcagt gccttttttc tcaacacagc ttacgcctgg caacaagaat atatcgttga
     8101 tacgcaaccc ggacattcca cagagcgtta cacctgggat agtgatcatc aacctgatta
     8161 caacgatatt ttgtcgcaac gtattcaaag tagccaaagg gcgctgggac tggaagtcaa
     8221 tctggcggaa gaaactcctg tggatgtgac cagcagtatg agtatgggct ggaattttcc
     8281 tttatatgaa caggttacaa ccggcccggt cgcggcatta cattacgatg gcacaaccac
     8341 ctcgatgtat aacgagtttg gcgacagtac taccacgctg accgatccgt tatggcatgc
     8401 cagcgtgagt agcttaggct ggcgtgttga ctcccggctt ggcgatctcc gaccctgggc
     8461 gcaaatcagc tataaccagc aatttggcga gaatatctgg aaggcgcaat caggcctgag
     8521 ccggatgacg gcgacaaacc agaacggcaa ctggctggat gtcaccgtag gcgctgatat
     8581 gttgctcaat caaaatattg ccgcctatgc tgcgctaact caggcagaaa ataccactaa
     8641 taatagcgac tatctgtata cgatgggggt tagcgccaga ttttaacgta acagtcacaa
     8701 ttgaaaccat tgaataacaa cagttgtggc gatagtgggt gctaacttac caaataataa
     8761 atttggtgaa taattgtcgc gtcattcatt cctgaactaa ggcatttcat tccgttctga
     8821 tggcatttca tgccgttttt ccccaagcat aaaatgcact tcgttatggt tgtcggcaga
     8881 gatttttcct ttttattact gcaggaatac tgccatgaca ccttcaaatt atcagcgtac
     8941 ccgctggctg acactcatcg gtactatcat tacccagttt gcgctggggt cggtttatac
     9001 ctggagcctg tttaatggcg cgctttctgc caagctggat gcgccggtaa gccaggtcgc
     9061 tttctctttc ggcttgttaa gtctggggct ggcaatttcg tcttctgttg cgggcaaatt
     9121 gcaggaacgt tttggcgtta aacgcgtcac cgtggcttcc ggcattttgc tgggattagg
     9181 cttcttcctg accgcgcatt ccaacaacct gatgatgctg tggttaagcg ccggtgtgct
     9241 ggtcggtctg gcggatggcg cgggttacct gctgacgctc tctaactgcg tgaagtggtt
     9301 cccggagcgt aaaggtctga tctccgcgtt cgctatcggt tcttatggtc tgggtagcct
     9361 gggtttcaaa tttatcgaca cgcagctgct cgaaacggtg ggtctggaaa aaacctttgt
     9421 gatttgggga gcgattgcgc tggtgatgat tgttttcggc gcaacgttaa tgaaagacgc
     9481 accaaaacag gaagtgaaaa ccagcaatgg tgtggtggag aaagattaca cgctggcaga
     9541 gtcgatgcgt aaaccgcagt actggatgtt agcggtaatg ttcctgactg cctgcatgag
     9601 cggcctgtac gtgattgggg tagcgaaaga tatcgcccaa agtctggcac accttgatgt
     9661 ggtttccgca gccaatgcag taacggttat ttccatcgct aacctttcag gtcgtctggt
     9721 gctgggcatt ctgtctgaca aaattgcccg tatccgtgtt atcaccattg gtcaggtgat
     9781 ttcgctggtg ggtatggcgg ccctgctgtt tgcaccatta aatgcagtga cgttctttgc
     9841 agcgattgcc tgcgtggcat ttaactttgg cggcactatt accgtctttc catcactggt
     9901 cagtgagttc ttcggcctca ataacctggc gaaaaactac ggtgtgattt acctcggttt
     9961 cggtatcggt agcatttttg gttcgattat cgcctcactg tttggcggtt tctatgtgac
    10021 cttctacgtg attttcgccc tgctgattct gtcattggcg ctttctacga cgattcgtca
    10081 gccagagcag aaaatgttgc gtgaggcgca tggctccctt taatgctcag ccgcgagcta
    10141 ctgggcttca gcttccgtgt gcatcaaacg catcaacgaa atgtataaac aatgactcgt
    10201 aaaatttggg ccagttaaca ggcccttttt actgcttttt gtaacgctaa agtctctttt
    10261 caaacttgca tttttgtaaa tttgtgcttc atgcacactc tttccccaca ctttttcgct
    10321 ttgctgtggt ctacttattc gcgcgtgtag attttactta tctgactacc tccgcacttt
    10381 tcccctgccg ggcctgaaaa gccgctaagc agggtgttat cacctgtttg tccagggttt
    10441 gtttgcatga gatacatcaa atcgattaca cagcagaagc tgagcttttt gcttgcaatc
    10501 tatattggcc tttttatgaa tggcgcggtt ttttatcgcc gcttcggcag ctatgcgcac
    10561 gattttaccg tctggaaagg cgtttctgct gttgttgaac tggccgccac cgtactggtg
    10621 accttctttt tactacgtct tctatcgctg tttggccgcc gcagctggcg tattctggcg
    10681 tcgctggtgg tgctcttttc cgcaggtgcc agctattaca tgaccttcct taatgtggtc
    10741 attggttatg gcatcatcgc ttccgtcatg accaccgata tcgacctgtc aaaagaagtt
    10801 gttggtctga actttattct ctggttaatc gccgttagtg cattgcctct tatccttatc
    10861 tggaataacc gctgtcgcta caccttgctc cgacaactgc gaaccccagg gcagcgtatt
    10921 cgcagcctgg tggtcgtcgt actggcgggt attatggttt gggcaccgat tcgtttgctg
    10981 gatatccagc agaagaaagt ggagagggcg accggcgttg atttgccgag ttatggcggt
    11041 gtcgtagcga actcttatct gccatcaaac tggctttctg cgttggggct gtatgcctgg
    11101 gcgcgggtcg atgaatcttc cgataataat tcattgctta atccggcgaa gaagtttaca
    11161 taccaggctc cacaaaacgt tgatgatact tatgtcgtgt ttatcatcgg tgaaaccacg
    11221 cgttgggacc atatgggtat tttcggctat gagcgtaata ccacgccgaa actggcccag
    11281 gagaaaaatc tggcggcgtt ccgtggttac tcctgtgata ccgcaaccaa actctcactg
    11341 cgttgcatgt ttgtacgtca ggggggcgcg gaagataatc cgcagcgcac attaaaagaa
    11401 cagaacattt tcgcggttct gaagcagtta ggattcagtt ctgacctcta cgctatgcag
    11461 agcgaaatgt ggttctacag caacacgatg gcagacaaca ttgcttatcg tgagcagatt
    11521 ggtgcggagc cacgtaatcg tggcaagccg gtagatgata tgttgctggt agacgaaatg
    11581 cagcaatcgc tagggcgcaa cccggatggt aagcatctga tcattctgca taccaaaggt
    11641 tcgcatttta actacaccca gcgttatccg cgtagcttcg cgcagtggaa gccggaatgt
    11701 attggtgttg atagcggctg taccaaagcg cagatgatca actcctatga caactcggtg
    11761 acctatgtgg atcactttat ctccagcgtg attgatcagg ttcgcgataa gaaagcgatt
    11821 gtgttctacg cagctgacca cggtgagtca attaatgaac gcgagcacct gcacggcacg
    11881 ccgcgtgaac tggcaccgcc ggagcagttc cgcgtaccga tgatggtctg gatgtcagat
    11941 aaatatctgg aaaatccggc caatgcgcag gcgtttgcgc agctgaaaaa agaagccgac
    12001 atgaaagtgc cacgccgtca cgtagagctg tacgacacca tcatgggttg tcttggctat
    12061 acttcaccgg atggtggaat taacgaaaac aacaactggt gtcacatccc gcagacaaaa
    12121 gtggcagcgg ctaactaagc ggcctgctga ctttctcgcc gatcaaaagg cattttgcta
    12181 ttaagggatt gacgagggcg tatctgcgca gtaagatgcg ccccgcattc ggtgattggc
    12241 gcagcctggt agcgcacttc gttcgggacg aaggggtcgg aggttcgaat cctctatcac
    12301 cgaccaaatt cgaaaagcct gctcaacgag caggtttttt tgcatttgta gctcatgagg
    12361 atgagaacct ccgggggcag ggaggctcga ctcgacgtag gcctgataag acgcgccagc
    12421 gtcgcatcag gcaacggcac cgcacttatt gtcggatgcg gcgcgaccgc cttattcgac
    12481 caacacacca cacattttcc tgccaacttt gcgccccata tctcactatc cattaacgga
    12541 tttgtgacag aaaccattgt ctttttttat atttgcttaa atcttttttg cgttacttat
    12601 ggctggcgct ggcattttcc gctgtgcgtt ttagcgttca tggtattaat cgctcagata
    12661 tccacctttt cctcagattt ttttcattaa atgcataaaa cttaatcacc aaatgttgct
    12721 aaatattaag ctgattgttc tggttatgtt ggagtagcac aaatttctct gctgcaaata
    12781 gctgtttgaa gtttttttaa tctttgtttg tggattctca ccgttggtgt aaatcccggt
    12841 tggctgtatt gacgttttca cattctgttg acagattgta ggtcacgagg ggcattttat
    12901 ggaggatccg cactgttaca ctgatgttaa ttagtacggc atccccacct cataacgttg
    12961 acccgaccgg gcaaaaaaca aaaaaggtca ggcagcgaca acccactgca aagggttaaa
    13021 acaacaaaca tcacaattgg agcagaataa tgcgtatttc cttgaaaaag tcagggatgc
    13081 tgaagcttgg tctcagcctg gtggctatga ccgtcgcagc aagtgttcag gctaaaactc
    13141 tggtttattg ctcagaagga tctccggaag ggtttaaccc gcagctgttt acctccggca
    13201 ccacctatga cgcctcttcc gtcccgcttt ataaccgcct ggttgaattt aaaatcggca
    13261 ccaccgaagt gatcccgggc ctcgctgaaa agtgggaagt cagcgaagac ggtaaaacct
    13321 ataccttcca tctgcgtaaa ggtgtgaagt ggcacgacaa taaagaattc aaaccgacgc
    13381 gcgaaatgaa cgccgatgac gtggtgttct cgttcgatcg tcagaaaaac gcgcaaaacc
    13441 cgtaccataa agtttctggc ggcagctacg aatacttcga aggtatgggc ttgccggagc
    13501 tgatcagcga agtgaaaaag gtggacgata acaccgttca gtttgtgctg actcgaccgg
    13561 aagcgccgtt cctcgctgac ctggcaatgg acttcgcctc tattctgtca aaagaatatg
    13621 ctgacgcgat gatgaaagcc ggtacaccgg aaaaactgga cctcaaccca atcggaaccg
    13681 gtccgttcca gttacagcag taccaaaaag attcccgtat ccgctacaaa gcgtttgatg
    13741 gctactgggg caccaaaccg cagatcgata cgctggtctt ctctattacc cctgatgctt
    13801 ccgtgcgtta cgcgaaattg cagaagaatg aatgccaggt gatgccgtac ccgaacccgg
    13861 cagacatcgc tcgcatgaag caggataaat ccatcaacct gatggaaatg ccggggctga
    13921 acgtcggcta cctctcgtat aacgtgcaga aaaaaccgct ggatgacgtg aaagttcgcc
    13981 aagcactaac ctacgcggtg aacaaagacg cgatcatcaa agcggtttat cagggcgcgg
    14041 gtgtatcagc gaaaaacctg atcccgccaa ccatgtgggg ctataacgac gacgttcagg
    14101 attacaccta cgatcctgaa aaagcgaaag ccttgctgaa agaagcgggt ctggaaaaag
    14161 gtttctccat cgacctgtgg gcgatgccgg tacaacgtcc gtataacccg aacgctcgcc
    14221 gcatggcgga gatgattcag gcagactggg cgaaagtggg cgtgcaggcc aaaatcgtca
    14281 cctacgaatg gggtgagtac ctcaagcgtg cgaaagatgg cgaacatcag acggtaatga
    14341 tgggctggac tggcgataac ggggacccgg ataacttctt cgccaccctg ttcagctgcg
    14401 ccgcctctga acaaggttct aactactcaa aatggtgcta caaaccgttt gaagatctga
    14461 ttcaaccggc gcgtgctacc gacgaccata acaaacgcgt tgaactgtac aagcaagcgc
    14521 aggtggtgat gcacgatcag gctccggcac tgatcatcgc tcactccacc gtgtttgaac
    14581 cggtacgcaa agaagtcaaa ggctatgtgg ttgatccatt aggcaaacat cacttcgaaa
    14641 acgtctctat cgaataatta aaagccatac aagactgatg gcaaaggcaa aactgcctga
    14701 tgtgctccgc ttatcaggcc tacgaaaatt ctgcaatgta ttgaatttgc acgattttgt
    14761 aggccggata aggcgttaac gccgcatccg gcataaacaa agcgcacttt gtcaacaatc
    14821 tgtatacccg gtggcgctgt gcctctgcgt gcagcgccat ccggcagcaa tacttcattc
    14881 tctgtccgac aagggcggga attgatttgt gagcaataca gacacgcagt tccaggctgc
    14941 gagtcactat agagaatccg ggttatgttg cagtttattc tccgacgttt gggactcgtc
    15001 atccccacgt ttatcggtat tacccttctc acatttgcct ttgtccacat gatcccgggc
    15061 gatccggtga tgatcatggc aggcgaacgt gggatctccc ctgagcgtca cgtgcagctg
    15121 ctggctgaac tcggcttaga taaaccgatg tggcagcagt atctccatta catttggggc
    15181 gtaatgcacg gcgatctagg catttcaatg aaaagccgaa ttccagtatg ggaagagttc
    15241 gtgccgcgct ttcaggccac gctggaactt ggcgtctgcg cgatgatttt tgctacggca
    15301 gtcggtattc cggttggtgt gctggctgcg gttaaacgcg gttccatttt cgatcacact
    15361 gcagttggcc tggcgctgac cggctactcg atgccgatct tctggtgggg catgatgctg
    15421 atcatgctgg tttcggtgca ctggaacctg acgcccgtct ccggtcgcgt gagcgatatg
    15481 gtgttcctcg atgactccaa tccgttaacc ggttttatgc taatcgacac cgccatctgg
    15541 ggtgaagacg gcaactttat cgatgccgtc gcccatatga tcttgcccgc cattgtgctg
    15601 ggtactattc cgctagcggt cattgtgcgt atgacacgct cctcgatgct ggaagtgctg
    15661 ggcgaggatt acatccgcac cgcgcgcgcc aaagggctaa cccgcatgcg ggtgattatc
    15721 gtccatgcgc tgcgtaacgc gatgctgccg gtagtgaccg ttatcggcct ccaggtggga
    15781 acattgctgg cgggggcgat tctgaccgaa accatcttct cgtggcccgg tctggggcgc
    15841 tggttgattg acgcactgca acgccgcgac tatccggtag tgcagggcgg cgtattgctg
    15901 gtggcgacga tgattatcct cgtcaacttg ctggtcgatc tgctgtacgg cgtggtgaac
    15961 ccgcgtattc gtcataagaa gtaaggggac atcatgtcac aggttactga aaataaagtg
    16021 attagcgcac cggtgccgat gaccccgtta caggagttct ggcactattt taaacgcaac
    16081 aaaggcgcgg tcgtcgggct ggtttacgtc gccatcgtgc tgttcatcgc gatctttgcc
    16141 aactggattg cgccttataa cccggcggat cagttccgcg atgcgctgct cgccccgcca
    16201 gcctggcagg aaggcggcag tatggcacac ttgctgggca ccgatgacgt cggtcgtgat
    16261 gtgctgtcgc ggctgatgta cggcgcgcgt ttatcgctgc tggttggctg tctggtggtg
    16321 gtgttgtcgc tgattatggg cgttattctc ggcctgatcg ccggttactt cggcggcctg
    16381 gttgataaca ttatcatgcg cgtggtcgat atcatgctgg cgctgccaag cctgctgttg
    16441 gcactggtgc tggtggcgat tttcggcccg tcgattggta acgccgcgct ggcgctgacc
    16501 ttcgttgcct tgccgcacta tgtgcgctta acccgcgccg ccgtgctggt ggaggtgaat
    16561 cgcgattacg tcaccgcctc tcgcgtggca ggtgccgggg cgatgcgcca gatgtttatt
    16621 aacatcttcc cgaactgcct tgcgccgctg attgttcagg cgtcgctcgg tttctctaac
    16681 gccattctcg atatggctgc tcttggtttc ctcggcatgg gggcacagcc gccaacgcct
    16741 gagtggggca ctatgctctc cgacgtgttg cagttcgcgc aaagcgcctg gtgggtcgtg
    16801 accttccctg gtctggcgat cctgttgacg gtgctggcat ttaacctgat gggtgacggt
    16861 ctgcgtgacg cgctcgatcc caaactaaag cagtaagagg ttcgagatgg cgttattaaa
    16921 tgtagataaa ttatcggtgc atttcggcga cgaaagcgca ccgttccgcg ccgtagaccg
    16981 catcagctac agcgtaaaac agggcgaagt ggtcgggatt gtgggtgagt ccggctccgg
    17041 taagtcggtc agttcactgg cgattatggg gctgattgat tatccgggcc gcgtaatggc
    17101 agaaaaactg gagtttaacg gccaggattt gcagcgtatc tcagaaaaag agcgccgcaa
    17161 cctggtgggt gccgaagtgg cgatgatctt ccaggacccg atgaccagcc ttaacccgtg
    17221 ctacaccgtg ggtttccaga ttatggaagc gattaaagtg catcagggcg gcaacaaaag
    17281 tacccgccgt cagcgggcga ttgacctgct gaatcaggtc ggtattcccg atccggcttc
    17341 gcgtctggat gtttacccgc atcagctttc cggcggcatg agccagcgcg tgatgatcgc
    17401 catggcgatt gcctgtcggc caaaactgct gattgccgat gaaccgacca ccgcgctgga
    17461 cgtgaccatt caggcgcaaa tcatcgaact actgctggag ctacagcaga aagagaacat
    17521 ggcgctggtg ttaattaccc atgacctggc gctggtggcg gaagctgcgc ataaaatcat
    17581 tgtgatgtat gcaggtcagg tagtagaaac gggcgatgca cacgccatct tccatgcgcc
    17641 gcgtcacccg tatactcagg cattgctgcg tgcgctgcca gaatttgctc aggacaaaga
    17701 acgtctggcg tcgttgccag gtgttgttcc cggcaagtat gaccgcccga acggctgcct
    17761 gcttaacccg cgctgcccct atgccactga cagatgtcgc gctgaagaac cggcgctgaa
    17821 tatgctcgct gatgggcgtc agtccaaatg ccattaccca cttgatgatg ccgggaggcc
    17881 cacactatga gtacgcaaga ggccacctcg caacaaccgc tgttgcaggc tatcgacctg
    17941 aaaaaacatt atccggtgaa gaaaggcatg ttcgtgccgg aacgtctggt gaaggcgctg
    18001 gacggcgttt cgtttaacct tgaacgtggc aaaacgctgg cagtagtggg cgaatctggc
    18061 tgcggtaaat cgaccctcgg tcggttgctg acgatgatcg aaacgcccac cggcggcgag
    18121 ctgtattatc aggggcagga tctgctcaag cacgatccgc aggcgcagaa gctgcgtcgg
    18181 cagaaaatcc agatcgtctt ccagaatccc tacggttcgc tgaatccgcg taagaaagtt
    18241 gggcaaattc tcgaagagcc gctgcttatt aacaccagct taagcaaaga acagcgtcgg
    18301 gaaaaagccc tgtcgatgat ggcaaaagtc ggcctgaaaa ccgagcacta tgaccgctat
    18361 ccgcacatgt tttccggcgg acaacgtcag cgtatcgcta ttgcccgtgg tctgatgctc
    18421 gacccggatg tggtgattgc cgatgagccg gtttctgcgc tggacgtctc agtgcgtgcg
    18481 caggtgctga atctgatgat ggatttgcag caggagttgg ggctttctta tgtctttatc
    18541 tcccacgacc tgtcggttgt ggagcacatt gctgatgaag tgatggtgat gtacctaggc
    18601 cgctgcgtgg agaaaggaac gaaggaccaa atcttcaata acccgcgcca tccgtacacc
    18661 caggcgctgc tctccgcgac gccacgcctg aacccggacg atcgtcgtga acgcatcaaa
    18721 ctcaccggtg aactgccaag cccgctgaat cctccgccgg gttgcgcctt caacgcccgc
    18781 tgtcgtcggc gcttcggccc ctgcacccag ttgcagccgc agctaaaaga ctacggaggt
    18841 caactggtag cttgttttgc tgttgatcag gatgaaaatc cgcagcgtta actcacaaaa
    18901 ccggggccat gcgctccggt ttactcaatc agcttcaaca gtggcgacaa gcatagcaaa
    18961 atgccgtaaa gcagaatcag ccaggcttta aatccgcgaa gtttttccag ttgtgcgact
    19021 ttatagatca ggaaaaacgg aatgaggcac gacacaatgc catataacgg gcttcccaac
    19081 tgaaagaaca ccagcactga tacacgaaac gaaacccaaa tcgtcaacgt aatgacgata
    19141 aaagcgcaga tcgccagagt cagcatgcgt gagttaattt tcttggtatc aataattcgg
    19201 cttaacagat tgagaataat gcctttaata gcctcgtgga aaccgagata aatgccaaag
    19261 aatgcggtca gtacggcaaa gatattcagc accgtagagg tgatatgaat gatatgtcca
    19321 gggatcacct gcgcggccag tgccagtgcc gagatatttt gttcaaaggc agaaacggct
    19381 tcttcgtggc taattgagaa ggtaaacgaa aaggcaaaaa acaggatcac cgcgatgagc
    19441 gtgatataac taattcggtg ggtacgcagc gcgagccggg ttgccagtac tttatctgct
    19501 tcccgtttac gataggcaat attcattggg ttaagtacct gaataaatac cgcagaaaag
    19561 aagcaaaatg gaatggtaag caggacatcg cggaaaaaga cggaggcttg cgggaaggcg
    19621 gttatattgg cgaaattcca gtgcgggatc atcgcaaaac cgaacacgac aataatccct
    19681 actttgacca ccaccattgg cccagaaatc ttaaacagta atcgttcacc accagacgca
    19741 atcgctatca gtacggcgaa aatagcgact ttataaagta gagattgtga aagatcggca
    19801 tcggttaaac cgaaggtttt caggtacgag gcgctgtcga aaaccacgga gagagagtag
    19861 ataaaaatcc cgtggataat catcaaaaag tagataaccc cgaggaaaat tccccagttc
    19921 ttccccaggt aatgactgat aatatcggtg tagtcattac aggaatcgct ttcagaaagg
    19981 gtttttaaat aaatgtcctg cactacccag gtgacaggat aagcaatgat cgctgcggta
    20041 ataaataccc aaattccctt caagccaatt tgtactggca tcagcacggt tccggcacca
    20101 atcgccatgc ctatgcataa taaaacccag ccgaagtcgt agcgtgtaaa cggcaatttc
    20161 tgattgtgtt tcgatagtgt gttgtgctgc ataaagtcct gtccagaaag aagtcggcct
    20221 cgcaaaaatg aataaaccca gtttctcttg cgcaacaggt ggagaagtcg aatacgcagt
    20281 atgtaaaaaa tgaggcaaca gattcactgg ggaggtcgcg aggaggcgca attctacaaa
    20341 attgcgcatt tcactaccag tctggagtca gcccggatat gaactgcaac tattgcaaat
    20401 aaagaaatta agctggaagg catcgcgcaa tgaggaagat tgccgggtgc tgacagtaaa
    20461 aagcaatcgt agaatcagcg tcaagccgga aggattccgg tgaggcgcaa tgttgcgggg
    20521 gcttaatccc tggtggcatt ggttgctgga gagagaaaac ccccgcacgt tgcaggtatg
    20581 tacctgacaa cataacgggg gctaatcttg aacctagacc actcaagaat agccgcgaaa
    20641 cgttgtcatt acaacacagg cggctatatg acgctcgcag agctgggcat ggccttctgg
    20701 catgatttag cggctccggt cattgctggc attcttgcca gtatgatcgt gaactggctg
    20761 aacaagcgga agtagcgtgt catgtgggcg tcaggctgcc gtaatggcaa tttgcgcccg
    20821 gaccaggccg caggggtgaa actctgcggc ctttttcgta agaaaaatgc aacggttgca
    20881 agcggataat attctgcgga atgcgcagca cagagtgaag atgagacgga ttccggaaca
    20941 gaaaaacagt cgtagaatca gcgtcaagcc ggaaggattc cggtgaggcg caatgttgcg
    21001 ggggcttaat ccctggtggc attggttgct ggagagagaa aacccccgca cgttgcaggt
    21061 atgcacctga caacaccacg ggggccattc ttgcatccaa acaatacaag aatagccgcg
    21121 aaacgttgtc attacaatac aggcggctat atgacgttcg cagaactggg catggccttc
    21181 tggcatgatt tagcggctcc ggtcattgct ggtattcttg ccagtatgat cgtgaactgg
    21241 ctgaacaagc ggaagtaacg tgccatgcgg gcgtcaggct gccgtaatgg caatttgcgc
    21301 ccggaccagg ccgcaggggt gaaactctgc ggcctttttc gtaagaaaaa tgcaacggtt
    21361 gcaagcggat aatattctgc ggaatgcgca gcacagagtg aagacgtgac ggattccgga
    21421 acagaaaaac agtcgtagaa tcagcgtcaa gccggaaggg ttccggtgag gcgcaatgtt
    21481 gcgggggctt tatccctggt ggcattggtt gctggagaga gaaaaccccc gcacgttgca
    21541 ggtatgcacc tgacaacatc acgggggcca ttcttgaacc tgaacaactc aagaatagcc
    21601 gcgaaacgtt gtcattacaa cacaggcggc tatatgacgc tcgcagaact gggcatggcc
    21661 ttctggcatg atttagcggc tccggtcatt gctggcattc ttgccagtat gatcgtgaac
    21721 tggctgaaca agcggaagta acgtgtcatg cgggcgtcag gctgccgtaa tggcaatttg
    21781 cgcccggacc aggccgcagg ggtgaaactc tgcggccttt ttcgtaagaa aaatgcaacg
    21841 attgcacgcg gataatattc tgcggaatgc gcagcacaga gtgaagacgt gacggattcc
    21901 ggaacagaaa aacagtcgta gaatcagcgg caagccggaa aggttccggt gaggcgcaat
    21961 gttgcggggg ctttatccct ggtggcattg gttgctggag agagaaaacc cccgcacgtt
    22021 gcaggtatgc acctgacaac atcacggggg ctaatcttga acccaaacaa ctcaagaata
    22081 gccgcgaaac gttggcatta caacacaggc ggctatatga cgctcgcaga actgggcatg
    22141 gccttctggc atgatttagc ggctccggtc attgctggca ttcttgccag tatgatcgtg
    22201 aactggctga ataagcggaa gtaacgtgtc atgcgggcgt caggctgccg taatggcaat
    22261 ttgcgcccgg atcaggccgc agaggttaaa ctctgcggcc tttttcgtgc ttactgcggg
    22321 taaggcaccc agtcgccgcc gttcaggcga acgtacggtt tatcttggta ttgaataact
    22381 actgcatttg agttctcgga gaccggtgct gtttgtggca acccactggt gagttttttc
    22441 cagtcaacat tgtcttcggt gaaaatcttg ccatcaagaa cgcgaaccac cagatcggag
    22501 atagccagga agctgctcgg ttggtcaatg acaatcggtg ccccctgatg tggtgccttc
    22561 atgccgaaga atttcacccc aacggggacg tctgtgatag acgggctagg gatatcacgt
    22621 aggccagata cctgcattct gtcgcccttc agcgcgccgc cgtgttccgg caccacgacc
    22681 accatcactt tacgacccga tttctccagt tcagtaaaga aggcgtccag ttcatcaaag
    22741 aatttctgcg cccgcgcttt gtaatccgct gttttgctga cccccggata atggttgccg
    22801 tcatgcagtg gaagcgtgtt gtagaacgtg gcactacggc tgtttttatc tttttcggta
    22861 acgtccagcc agcggttaag cacggcggta tcgtcataaa ccggcgaacc atcaaagccc
    22921 agcaaaataa ccggcagatt tgtttgatcc atcaattcag tctgcatgcc gccattttcg
    22981 cgaacttctt tcaaaaaacc gccgaactgg ccgttatgtc ccatcatcag gtgctgggta
    23041 aagcccagtt tcgaaaggtt atcaaacaga tagcagtcgt tatttgccgg ttgatacaga
    23101 ttagtgtgcg aagtctgccc gcagctggcg cgcagtaaac ggatcgccgc cgggccactg
    23161 taggaggtgg cggagttaaa gttcttgaaa tcaatatcga aatgcgacca cagtggatgc
    23221 gacatcaacc cggcggcttc tatatccgac caggaaagcg aacagatgtt aatcaccagt
    23281 agttcaaatg gctgagcatc agcgggcagc gaagacggga aggtcgattt acgtttcgcc
    23341 tccgcgttat agaaattatt cagccaggcg ttaaggttcg ccgttgttgg cggtgcagtt
    23401 tgtgcgggca tatcacccac taccggtgcg ccacccgtcg ccgcaacggt tgccgctgcg
    23461 ttaccacccg tcgttgttac agtggtcgtc ggttgtccgg ctggccacaa ggagaaactt
    23521 ggtcccgcca aggtaagtac gttcagccat agcagtatgg caaccacaaa aacggtaatg
    23581 cgaatccatt gtgacaggaa taaccaggcc actaataaaa caaaaatggc cccaatcatc
    23641 tgccagttaa taaagcgtgt gacaaggtcg attaaataat cggtactgaa ccccgccacc
    23701 tgcgaaccct ggctcattat gctttccggg ccaggcaacc agatgtcatg ccagaacaaa
    23761 gcaaagccga tcggcagggc aatccagtgg cgcaagcgat gcaggctgta acgcggaatg
    23821 ggcatcagca gaaacgcggc aaacaccaaa ttgaggagcg ggtggaagtt aagatatccc
    23881 gcccacaaca ggccaaactt aaccagaaaa tagaagttcc agccggaaag gccgcgccag
    23941 tattgccaga gggaagaagg catggcggta ttttgcgtaa attgagtcat tttttggttg
    24001 ccctggcttt ttccgtgcgg cgtaacgtcc cggccggttt aacataacga ggtttagcaa
    24061 agaacaaacg taaggtgtcg cgaatgcgtc gcaaagagtg ccgcgccaga tagcccagcg
    24121 ggaaaaatat cagtgcgcaa acgataataa tttcaatgat atcgctgatg gtcatcatga
    24181 tgagcgctcc acagcatcat ctaacagtcg catgggttct ggtattcgtc gccagtggcg
    24241 accatcgtgc tcggcattga tgaccggttt agaactttgc gttaaaggca gcggcattcc
    24301 ccattgttct ggggcaagca ggcgcatctg caccagctcg gcactgattt gatcatcttc
    24361 aaaccagacc atacggtttg agaaaatgtc gccagtaggc aatgggaaaa tatgattcaa
    24421 cgcggtatcc agatcgttaa tccgacagaa tgagagaaac agcaccagcc gattaccgcc
    24481 aatggtcatg atatcgccgg tacggttagg gcgacatagc gtcagggctt gctcaacgcg
    24541 gatacccggt accggacgta gggcaaccag aacgccttta ccgtgggcag gcaagagagg
    24601 gttattcatc atgttgttga cggcattaca gaacacatcc cacttctgga aaccacgcag
    24661 tttgagcggc tgggtcattg acagcaaggt agtgatatct tccggtacat agcgactaaa
    24721 cttctgccct tgcacgcttt cgatcatcgt cagacaacgg gagagtggcg cattccacgg
    24781 aataaccata tttgcaccgc aggccaataa caaacgttca tcggtggcgc gcaggctagc
    24841 ggtattttcc cgcacgagga ttttcatcgc actaccgcgc tggcgacgca gggtatgaat
    24901 gctgcgggcc agtggctcga tttgcgcatt ttgctgtaaa gaaaagacca ccgtcgccgc
    24961 ctgagcggta cgggcttcat tgaacaggac ttcgttattg ttgaacagtt gccagtgttc
    25021 cgatagcggc ggcgcacctt ccagtacagc aacattactc agaatgcgtt tttcgtcgct
    25081 gcgtggttgg atctccgcct cttcgctttg aactaatgtc cagataccat tttgttgctg
    25141 aacgctaagc tgctgacggg cgctgacccc tttttcgttg caccagaagg caatatccag
    25201 caaatgttgg tcaccctgaa aacgcaaact ggcaagacca aaaagtgaac ggtattcctc
    25261 aagcaataat gaaaattgtt tatcgttatt atttccggga ttaattacca aaagcgaaca
    25321 atggtttaac ctgctccatt tattcatttt atccaaccat gagcgaagcc gctcggcagg
    25381 aatattttgc catgcgttat ttgcgcaaac aagaataaaa aggtaattat ggggatcaat
    25441 agaacactgc aaatcacggg ccaaatagta tagacctttt tcatgattta gcattgaaaa
    25501 taattttatt ttttccggcc cttgagaatc atctaattga aagattttcg ccggatcgct
    25561 gtccatgcta atgaccgcga cgtgtgcggt ttcagcctgg gatgcaattg tttgattcgc
    25621 cagactgata gcatcttcat ggcgatcgac gttaaaccac cagacgccgc ctgctggcat
    25681 atgtcgcagc tcatcccata atgatgagat accgatagag aatacagggt ccacaatgtc
    25741 cctcatgtca ccattttgtc tgattatcag tttactagcg aaagctaaga aataaaccta
    25801 ccattgaaat taaaacttat cagatttagc atgtgaatca atttagaagg tcacaagacg
    25861 tgtgatttta gtcttttcgt ttttaacttt ttctgttgcg taacggaaag tcaaaaagtg
    25921 agcaaattcc cgtctcgccg taaacaataa accggcgaaa tgctcacggt tagaattgtc
    25981 tcatgaatgg tacggttatt tcatagggat caagcaaaat gaataacaat gaaccagata
    26041 ctctgcctga tcccgcgata ggctatatct tccagaatga tattgtggcg ttaaagcagg
    26101 cattttcact gcctgatatt gattatgccg atatttccca acgcgaacag ttggccgcgg
    26161 cattaaaacg ctggccgttg ctggcagagt ttgcgcaaca aaagtagggg attggtgaat
    26221 ggccgtactg ggattgcagg gggtgcgggg aggcgtgggg acaacaacca tcaccgccgc
    26281 attagcctgg tcattacaaa tgttgggaga aaatgtcctg gtggtcgatg cctgcccgga
    26341 caacttgttg cgcctgtcat ttaatgttga ttttacccac cgtcagggct gggccagagc
    26401 gatgctggat ggccaggact ggcgtgacgc tgggttgtgc tacacctcgc agctcgattt
    26461 gctgcctttt ggtcagttat ccattgaaga acaagaaaat ccacagcact ggcaaacccg
    26521 gctgagcgat atttgctccg gcttacagca actaaaagcc agcgggcgtt accagtggat
    26581 tttaatcgac ttaccgcgtg atgcctcgca gataacccac cagctgctga gtttgtgcga
    26641 gcactcgctg gcaatcgtca atgtggatgc caactgccat atccgactgc atcagcaagc
    26701 gctgccggat ggcgcacata ttttgattaa tgacttccgt attggcagtc aggttcagga
    26761 cgatatttac cagctttggt tgcaaagcca gcgccgatta ctgccgatgc tcattcatcg
    26821 tgatgaagcg atggctgaat gcctggcggc taagcaacca gtaggtgaat atcgcagtga
    26881 tgcgctggcg gctgaagaga tactgacgct ggcgaactgg tgcctgttga actactccgg
    26941 gctgaaaacg ccagtcggga gtgcatcatg agtatcctga cccggtggtt gcttatcccg
    27001 ccggtcaacg cgcggcttat cgggcgttat cgcgattatc gtcgtcacgg tgcgtcggct
    27061 ttcagcgcga cgctcggctg tttctggatg atcctggcct ggatttttat tccgctggag
    27121 cacccgcgct ggcagcgtat tcgcgcagaa cataaaaacc tgtatacgca tatcaacgcc
    27181 tcgcgtccgc gtccgctgga cccggtccgt tatctcattc aaacatgctg gttattgatc
    27241 ggtgcatcgc gcaaagaaac gccgaaaccg cgcagcgggc attttcaggt ctgcaaaata
    27301 ttcgtggacg ttaccatcaa tggatgaacg agctgcctga gcgcgttagc cataaaacac
    27361 agcatctgga tgaaaaaaaa gagctcggtc atttgagtgc cggggcgcgg cggttgatcc
    27421 tcggtatcat cgtcaccttc tcgctgattc tggcgttaat ctgcgttact cagccgttta
    27481 acccgctggc gcagtttatc ttcctgatgc tgctgtgggg ggtagcgctg atcgtacggc
    27541 ggatgccggg gcgcttctcg gcgctaatgt tgattgtgct gtcgctgacc gtttcttgcc
    27601 gttatatctg gtggcgttac acctctacgc tgaactggga cgatccggtc agcctggtgt
    27661 gcgggcttat tctgctcttc gctgaaacgt acgcgtggat tgtgctggtg ctcggctact
    27721 tccaggtagt atggccgctg aatcgtcagc cggtgccatt gccgaaggat atgtcgctgt
    27781 ggccgtcagt ggatatcttt gtcccgactt acaacgaaga tctcaatgtg gtgaaaaata
    27841 ccatttacgc ctcgctgggt atcgactggc cgaaagacaa actgaacatc tggatcctcg
    27901 atgacggcgg catggaagag tttcgccagt ttgcgcaaaa cgtgggggtg aaatatatcg
    27961 cccgcaccac tcatgaacat gcgaaagcgg gcaacatcaa caatgcgctg aaatatgcca
    28021 aaggcgagtt cgtgtcgatt ttcgactgcg accacgtacc aacgcgatcg ttcctgcaaa
    28081 tgaccatggg ctggttcctg aaagagaaac agctggcgat gatgcagact ccacaccact
    28141 tcttctcgcc ggacccgttt gaacgcaacc tgggacgttt tcgtaaaacg ccgaacgaag
    28201 gcacgctgtt ctatggtctg gtgcaggatg gcaacgatat gtgggacgcc actttcttct
    28261 gcggttcctg tgcggtgatt cgtcgtaagc cgctggatga aattggcggc attgctgtcg
    28321 aaaccgtgac tgaagatgcg catacttctc tgcgtttgca ccgtcgtggc tatacctccg
    28381 cgtatatgcg tattccgcag gcggcggggc tggcgaccga aagtctgtcg gcgcatatcg
    28441 gtcagcgtat tcgctgggcg cgcgggatgg tataaatctt ccgtctcgat aacccgctca
    28501 ccggtaaagg actgaagttt gctcagcggc tgtgctacgt caacgccatg ttccacttct
    28561 tgtcgggcat tccacggctg atcttcctga ctgcgccgct ggcgttcctg ctgcttcatg
    28621 cctacatcat ctatgcgcca gcgttgatga tcgccctatt cgtgctgccg catatgatcc
    28681 atgccagcct gaccaactcg aagatccagg gcaaatatcg ccactctttc tggagtgaaa
    28741 tctacgaaac ggtgctggcg tggtatatcg caccaccgac gctggtggcg ctgattaacc
    28801 cgcacaaagg caaatttaac gtcaccgcca aaggtggact ggtggaagaa gagtacgtcg
    28861 actgggtgat ctcgcggccc tacatcttcc ttgttctact caacctggtg ggcgttgcgg
    28921 taggcatctg gcgctacttc tatggcccgc caaccgagat gctcaccgtg gtcgtcagta
    28981 tggtgtgggt gttctacaac ctgattgttc ttggcggcgc agttgcggta tcggtagaaa
    29041 gcaaacaggt acgccgatcg aaccgcgtgg agatgacgat gcccgcggca attgcccgcg
    29101 aagatggtca cctcttctcg tgtaccgttc aggatttctc cgacggtggt ttggggatca
    29161 agatcaacgg tcaggcgcag attctggaag ggcagaaagt gaatctgttg cttaaacgcg
    29221 gtcagcagga atacgtcttc ccgacccagg tggcgcgcgt gatgggtaat gaagttgggc
    29281 tgaaattaat gccgctcacc acccagcaac atatcgattt tgtgcagtgt acgtttgccc
    29341 gtgcggatac atgggcgctc tggcaggaca gctacccgga agataagccg ctggaaagtc
    29401 tgctggatat tctgaagctc ggcttccgtg gctaccgcca tctggcggag tttgcgcctt
    29461 cttcggtgaa gggcatattc cgtgtgctga cttctctggt ttcctgggtt gtatcgttta
    29521 ttccgcgccg cccggagcgg agcgaaacgg cacaaccatc ggatcaggct ttggctcaac
    29581 aatgatgata acgcgatgaa aagaaaacta ttctggattt gtgcagtggc tatggggatg
    29641 agtgcgttcc cctctttcat gacgcaggcg acgccagcaa cgcaaccact gatcaatgct
    29701 gagccagctg tagccgccca gacggaacaa aatccgcagg tggggcaagt gatgtcaggc
    29761 gagcagggag ctgatgcccc catcgtggcg cagaacggtc cttcgcgtga tgtgaagctg
    29821 acctttgcgc aaatcgcgcc gccgccgggc agcatggtgc tacgtggtat taacccgaac
    29881 ggcagcattg agtttggtat gcgcagcgat gaagtggtga cgaaggcgat gctcaacttc
    29941 gaatacacgc catcgccatc gttactgcct gtccagtcgc agttaaaggt ttatctcaat
    30001 gatgagctga tgggcgtgct gccagtgacc aaagaacagt tgggtaaaaa aacgctggcg
    30061 caaatgccca ttaatccact gtttattacc gacttcaacc gtgtgcggct ggagtttgtc
    30121 ggccattatc aggacgtgtg cgaaaacccg gccagcacca cgctttggct ggatgttggg
    30181 cggagcagtg gactggatct gacctatcag accctgaatg tgaagaatga cctgtcacac
    30241 ttcccggtgc cattctttga cccgcgcgat aaccgtacta acaccttgcc gatggtcttt
    30301 gcgggtgcgc cggatgttgg gctgcaacaa gcatctgcca ttgtcgcctc gtggtttggt
    30361 tcgcgttctg gctggcgtgg gcagaacttc ccggtgctct ataaccaatt gccggatcgc
    30421 aatgccattg tctttgcaac caacgacaaa cggccggact tcctgcgcga tcatccggcg
    30481 gtaaaagccc cggtgattga gatgattaac catccgcaga atccttacgt caaactgctg
    30541 gtggtgtttg gtcgtgacga caaagacctg ttgcaggcag cgaaaggtat cgctcagggt
    30601 aacattctgt tccgtggtga aagcgtggta gtgaatgaag tgaaaccgct gctaccgcgt
    30661 aagccgtacg atgcgccgaa ctgggtacgt accgatcgtc cggtcacatt tggcgaactg
    30721 aaaacctatg aagaacagtt acaatccagc ggccttgagc cagcagcgat taacgtttcg
    30781 ctaaacctgc cgccggatct ctacctgatg cgcagtaccg gcattgatat ggatattaat
    30841 taccgctaca ccatgccgcc ggtgaaagac agttcgcgga tggatatcag cctgaataac
    30901 cagttcctgc aatccttcaa cctgagcagc aaacaggagg cgaaccgtct gctgctgcgt
    30961 attccggtat tacaaggttt gctggatggc aaaacagatg tctctattcc ggcgctgaaa
    31021 ctgggcgcga ccaaccagct gcgcttcgac tttgagtata tgaacccgat gccaggcggt
    31081 tcggtagata actgtattac cttccagccg gtgcagaatc atgtggtgat tggtgacgat
    31141 tccaccatcg acttctcgaa gtattaccac ttcatcccga tgccggatct acgcgccttt
    31201 gctaacgcgg gcttcccatt cagccggatg gcggatctgt cgcaaaccat caccgtgatg
    31261 ccgaaagcgc ctaacgaagc acagatggaa acgttgctga atactgttgg ttttattggc
    31321 gcacagacgg gcttcccggc gattaatctg acggtgaccg atgatggcag caccattcag
    31381 ggcaaagatg ccgacatcat gatcatcggt ggtatcccgg acaaactgaa agacgataag
    31441 cagatcgacc tattggtgca ggcgaccgaa agctgggtga aaacaccgat gcgccagacc
    31501 ccgttccccg gcattgtgcc ggacgagagc gatcgcgcgg cagaaaccca gtcaactctg
    31561 acctcttccg gtgcgatggc ggcggtgatt ggcttccagt cgccgtataa cgaccagcgc
    31621 agcgtgattg cgctgttggc agatagccca cgcggttatg aaatgcttaa cgatgcggtg
    31681 aacgatagcg gcaaacgcgc caccatgttc ggttcggtcg cggtgatccg cgagtccggt
    31741 atcaacagcc tacgtgttgg cgacgtttat tacgtaggtc atctgccgtg gttcgagcgc
    31801 ttgtggtatg cgctggcaaa ccatccgatt ctgctggcgg tgctggcgac tatcagtgtg
    31861 atattgctgg catgggtact gtggcgtctg ctgcgaatta ttagtagtcg tcgtcttaac
    31921 ccggataacg agtaattgaa gatgaatgtg ttgcgtagtg gaatcgtgac gatgctgttg
    31981 ttggctgcct ttagtgttca ggcagcctgt acctggcctg cctgggagca gtttaaaaag
    32041 gattacatca gtcaggaagg gcgcgtcatc gaccccagcg acgcgcgcaa aatcaccacc
    32101 tccgaagggc aaagttacgg tatgttcttt gccctggcgg ctaacgaccg tgcagctttc
    32161 gataatattc tcgactggac gcagaacaat ctcgctcagg gttctttaaa agaacgtttg
    32221 cccgcctggc tgtggggcaa gaaagagaac agtaagtggg aagtgctgga cagcaattcg
    32281 gcctccgatg gtgatgtctg gatggcctgg tcgttgctgg aggcggggcg tttgtggaaa
    32341 gagcagcgtt ataccgacat cggcagcgcg ttgctaaaac gtatcgcgcg ggaggaagtg
    32401 gtgacggtgc ctgggctggg ttccatgttg ttaccgggca aagtgggttt tgctgaggat
    32461 aacagctggc gttttaaccc cagctacatg ccgccgacgc tggcgcagta tttcacccgc
    32521 tttggcgcgc cgtggaccac gctgcgcgaa accaatcaac atttattgct ggaaaccgcc
    32581 ccgaaaggct tttcgccaga ctgggtgcgc tatgagaaag acaaaggctg gcagctaaaa
    32641 gccgaaaaaa cattgatcag cagctacgac gctatccgcg tttacatgtg ggtaggcatg
    32701 atgcctgaca gcgatccgca aaaagcgcgg atgctcaacc ggtttaaacc gatggcgaca
    32761 ttcactgaga aaaacggtta tccgccggaa aaagtggatg tggctacggg gaaagcgcag
    32821 ggtaaaggac cggtcggttt ttctgccgcc atgctgcctt ttttacaaaa ccgcgatgcg
    32881 caggcagttc agcgccagcg cgtggccgat aactttcccg gcagcgatac ctattacaac
    32941 tatgtgctga ccctgtttgg acaaggctgg gatcaacacc gtttccgctt ctcgacaaaa
    33001 ggtgagttat tacctgactg gggccaggaa tgcgcaaatt cacactaaac atattcacgc
    33061 tttccctcgg tctggccgtc atgccgatgg tcgaggcagc accaaccatc agcagaccct
    33121 gcggatggac agcggcaaca ctaatgccgt gcgcgggctg gcgaatattt accgccagca
    33181 gtcgccagaa aaagctgaag cgtttatcgc ctcgctctct gccagccagc ggcgcagcat
    33241 tgatgatatc gaacgcagcc tgcaaaacga ccgtctggca cagcaggcag aggtactgga
    33301 aaaccagggc aaatgggcgc aggcggcagc acttcagcgg caacgactgg cgctgggccc
    33361 cggcagcgta tggattactt accgactttc gcaggatctc tggcaggccg gacaacgcag
    33421 ccaggccgat acgttaatgc gcaatctggc gcagcagaag ccgaacaacc cggagcaggt
    33481 ttacgcttac gggctgtacc tctcaggtca caaccaggac agagcggcgc tggcgcatat
    33541 caacagcctg ccgcgcgcgc agtggaacag caatattcag gagctggtta atcgactgca
    33601 aagcgatcag gtgctggaaa ccgctaatcg cctgcgagaa agcggcaaag aggctgaagc
    33661 ggaagcgatg ctgcgtcagc aaccaccttc cacgcgtatt gacctcacgc tggctgactg
    33721 ggcgtaacaa cgacgtgatt acaccgccgc ccgcactgca tatcagaatg tcctgacgcg
    33781 ggagccaact aacgccgatg ccattcttgg tctgacggaa gtggatattg ctgccggtga
    33841 caaagcggcg gcacgtagcc agctggcgaa actgcctgcc accgataaca cctcgctgaa
    33901 cactcagcgg cgcgtggcgc tggcacaggc gcagcttgga gataccgcag cggcgcagca
    33961 gacgtttaat aagttgatcc cgcaggcaaa atctcagcca ccgtcgatgg aaagcgcgat
    34021 ggtactgcgc gacggtgcga agtttgaagc gcaggcgggc gatccaaagc aggcgctgga
    34081 aacctacaaa gacgccatgg tcgcatccgg tgtgactacg acgcgtccgc aggataacga
    34141 cacctttacc cgactgaccc gtaacgacga gaaagatgac tggctgaaac gcggcgtgcg
    34201 cagcgatgcg gcggacctct atcgccagca ggatcttaac gtcacccttg agcacgatta
    34261 ctggggttcg agcggcaccg gtggttactc cgatctgaaa gcgcacacta ccatgttgca
    34321 ggtggatgcg ccgtattctg acgggcggat gttctttcgc agtgatttcg tcaatatgaa
    34381 cgtcggcagt ttctccacta atgccgatgg caaatgggat gacaactggg gcacctgtac
    34441 attacaggac tgtagcggca accgcagcca gtcggattcc ggtgccagcg tggcggtcgg
    34501 ctggcgaaat gacgtctgga gctgggatat cggtaccacg ccgatgggct tcaacgtggt
    34561 ggatgtggtc ggcggcatca gttacagcga tgatatcggg ccgctgggtt acaccgttaa
    34621 cgcccaccgt cggcccatct ccagttcttt gctggccttt ggtgggcaaa aagactcccc
    34681 gagcaatacc gggaaaaaat ggggtggcgt acgtgccgac ggtgtggggc taagtctgag
    34741 ctacgataaa ggtgaagtaa acggcgtctg ggcatcgctt agtggcgacc agttaaccgg
    34801 taaaaatgtc gaagataact ggcgcgtgcg ctggatgacg ggctattact ataaggtcat
    34861 taaccagaac aatcgccgcg tcacaatcgg cctgaacaac atgatctggc attacgacaa
    34921 agatctgagt ggctactcac tcggtcaggg cggttactac agtccgcagg aatacctgtc
    34981 gtttgccata ccggtgatgt ggcgggagcg cacggaaaac tggtcgtggg agctgggggc
    35041 gtctggctcg tggtcgcatt cacgcaccaa aaccatgccg cgttatccgc tgatgaatct
    35101 gatcccgacc gactggcagg aagaagctgc gcggcaatcc aacgatggcg gcagcagtca
    35161 gggcttcggc tacacggcgc gggcattact tgaacgacgt gttacttcca actggtttgt
    35221 tggtacggca attgatatcc agcaggcgaa agattacgca cccagccatt tcctgctcta
    35281 cgtacgttat tccgccgccg gatggcaggg tgacatggat ttaccgccgc agccgctgat
    35341 accttacgcc gactggtaag ttttcagata gcgcctctct taatgccgct gcgatcgggt
    35401 atactcgggc ggcaatctgg gatttccggg gggagacaat ttgcgcgtaa gtcgctcgtt
    35461 aacaatcaag cagatggcaa tggtggcagc cgttgtcctg gtgttcgttt ttattttttg
    35521 caccgttttg ctgttccatc tggtccagca gaatcgctat aacacggcta cgcaactgga
    35581 aagcattgct cgctctgtcc gcgaaccctt atcttcagct attttgaaag gcgatattcc
    35641 cgaagcggaa gctattcttg ccagcattaa accggcaggc gtggtcagcc gtgccgatgt
    35701 agtgctgcct aaccagttcc aggcgctgcg taaaagtttt attccagagc gtccggtgcc
    35761 ggtaatggtt actcgcctgt ttgagctacc ggttcaaatc tcgctgggcg tctactcgct
    35821 cgaacgtccg gcaaatccgc agccaatagc ctatctggtg ctacaggcgg attccttccg
    35881 tatgtataag ttcgtgatga gcactctctc aacgttagtg accatttact tacttttgtc
    35941 gcttatcctg accgtcgcca tcagctggtg cattaaccgc ctgattttgc atccgttacg
    36001 caatattgct cgcgaactta acgccatccc agcccaggag cttgttggtc accaactggc
    36061 attaccgcgt ctgcatcagg acgatgaaat cggtatgttg gtgcgcagtt acaacctcaa
    36121 ccagcaattg ctgcagcgcc attatgaaga acagaacgaa aatgcgatgc gcttcccggt
    36181 gtcggatttg ccgaacaaag ccttgctgat ggagatgctg gagcaggttg tcgcgcgtaa
    36241 acaaaccacc gcgctgatga tcatcacctg tgaaaccctg cgtgatactg cgggcgtgct
    36301 gaaagaggcg caacgagaaa ttctgctgct gacgctggtg gaaaaactca aatcggtact
    36361 gtcgccacgt atgatcctcg cgcagattag cggttatgac tttgctgtca ttgccaacgg
    36421 tgtacaggaa ccgtggcacg caatcacttt gggtcagcaa gtactcacta tcatgagcga
    36481 gcgcctgccg attgaacgta ttcaactccg tccgcactgt agcattggcg tggcgatgtt
    36541 ctacggcgat ctcaccgccg aacagcttta cagtcgcgct atttctgcgg catttaccgc
    36601 tcgccataaa ggcaagaatc agattcagtt ctttgatccg cagcagatgg aagcagccca
    36661 gaagcggttg acggaagaga gcgatatcct taatgcactg gaaaatcatc agtttgcaat
    36721 ttggttacag ccacaggtcg agatgaccag cggtaaactg gtcagtgcgg aagtgttact
    36781 gcgtatccag caaccggatg gcagttggga cctgccggat ggcttaatcg atcgcattga
    36841 gtgctgtggg ctgatggtta ccgtcggtca ctgggtgctg gaagagtcct gtcgattgct
    36901 tgcagcctgg caagagcgcg gcattatgct gcccttgtcg gtaaacttct ctgcgctgca
    36961 actgatgcac ccgaatatgg tggcggatat gctggaactg ttaacccgct atcgcattca
    37021 gccgggaaca ctgattctgg aagtgacaga aagccgacgt attgacgacc ctcatgctgc
    37081 ggtggcaatc ctccgtccgc tgcgcaatgc cggagttcgg gtggcgctgg atgatttcgg
    37141 catgggctac gcagggctgc gtcagctgca gcatatgaaa tcgttgccaa tcgacgtact
    37201 gaaaatcgac aaaatgtttg ttgaaggctt gccggaagat agcagcatga ttgctgcaat
    37261 tatcatgctg gcgcagagcc tgaacttaca aatgattgcc gaaggcgtgg agactgaagc
    37321 acaacgagac tggctggcaa aagcgggcgt tggtattgcc cagggcttcc tttttgctcg
    37381 cccactccct attgaaatct tcgaagagag ttacctggaa gaaaagtagc taccccaaac
    37441 tgattacaaa actttaaaaa gtgctggttt gtgcgagcca gctcaaactt tttaaccttt
    37501 ttgtttcaat tatgatccag gtacatttct gtgatgttgt ctgggtgtta ttttaaggcc
    37561 gcaggtaccc ccccataacc ttacaagacc tgtggtttta ctaaaggaca ccctatgaaa
    37621 acctctctgt ttaaaagcct ttactttcag gtcctgacag cgatagccat tggtattctc
    37681 cttggccatt tctatcctga aataggcgag caaatgaaac cgcttggcga cggcttcgtt
    37741 aagctcatta agatgatcat cgctcctgtc atcttttgta ccgtcgtaac gggcattgcg
    37801 ggcatggaaa gcatgaaggc ggtcggtcgt accggcgcag tcgcactgct ttactttgaa
    37861 attgtcagta ccatcgcgct gattattggt cttatcatcg ttaacgtcgt gcagcctggt
    37921 gccggaatga acgtcgatcc ggcaacgctt gatgcgaaag cggtagcggt ttacgccgat
    37981 caggcgaaag accagggcat tgtcgccttc attatggatg tcatcccggc gagcgtcatt
    38041 ggcgcatttg ccagcggtaa cattctgcag gtgctgctgt ttgccgtact gtttggtttt
    38101 gcgctccacc gtctgggcag caaaggccaa ctgattttta acgtgatcga aagtttctcg
    38161 caggtcatct tcggcatcat caatatgatc atgcgtctgg cacctattgg tgcgttcggg
    38221 gcaatggcgt ttaccatcgg taaatacggc gtcggcacac tggtgcaact ggggcagctg
    38281 attatctgtt tctacattac ctgtatcctg tttgtggtgc tggtattggg ttcaatcgct
    38341 aaagcgactg gtttcagtat cttcaaattt atccgctaca tccgtgaaga actgctgatt
    38401 gtactgggga cttcatcttc cgagtcggcg ctgccgcgta tgctcgacaa gatggagaaa
    38461 ctcggctgcc gtaaatcggt ggtggggctg gttatcccga caggctactc gtttaacctt
    38521 gatggcacat cgatatacct gacaatggcg gcggtgttta tcgcccaggc cactaacagt
    38581 cagatggata tcgtccacca aatcacgctg ttaatcgtgt tgctgctttc ttctaaaggg
    38641 gcggcagggg taacgggtag tggctttatc gtgctggcgg cgacgctctc tgcggtgggc
    38701 catttgccgg tagcgggtct ggcgctgatc ctcggtatcg accgctttat gtcagaagct
    38761 cgtgcgctaa ctaacctggt cggtaacggc gtagcgacca ttgtcgttgc taagtgggtg
    38821 aaagaactgg accacaaaaa actggacgat gtgctgaata atcgtgcgcc ggatggcaaa
    38881 acgcacgaat tatcctctta atctcaccac ttatgcccgc actcccttcc ccgagtgcgg
    38941 gtgtttgcgc ataattgacc cctgtgcctg aacttaccac ctgggtgagt tcaggtttat
    39001 ttcacttctt ccgtgacatt ttcatgttct tgcggtctaa cacgaagtgt ttttaacgtc
    39061 atttagactg accgacaacg ggcagttttt ttattaccag gatagttgat caggggttca
    39121 catgcagggc ataaaaattc gacttttagc gggcggtttg ctgatgatgg ccactgctgg
    39181 ctatgtgcag gcagatgcgc tccagcctga tccagcatgg caacagggga cgctttccaa
    39241 cggtttacag tggcaagtgc tgaccacccc ccagcgtccc agcgatcgtg ttgaaattcg
    39301 cctgctggtt aataccggtt cgctcgccga aagtacacaa cagagcggtt acagtcacgc
    39361 catccctcgt attgcgctaa cgcaaagcgg tggccttgac gcagcacagg cgcgttcatt
    39421 gtggcagcag gggatcgacc ctaaacgccc gatgccgccg gtaattgtct cttatgacac
    39481 cacgctgttt aatctgagtt tgcccaataa ccgtaacgat ttgctgaaag aagcgctctc
    39541 ttatctggca aatgccactg gcaaattgac catcacacca gaaaccatca accacgcgct
    39601 gcaaagtcag gacatggtgg caacctggcc tgccgatact aaagagggct ggtggcgcta
    39661 tcgtctgaaa gggtcaacct tgttaggtca cgatcctgcc gatccgctga aacaacccgt
    39721 tgaagcggaa aaaattaaag atttctatca gaaatggtac accccggatg caatgacgct
    39781 actggtggtg ggaaacgtgg atgcgcgctc ggttgtcgac caaatcaaca aaacgtttgg
    39841 cgaactgaaa ggcaaacgtg aaacgccagc tccggtgccg acgctttctc cgctgcgtgc
    39901 ggaagcggtg agtattatga ctgacgcggt gcgtcaggac cggttatcta tcatgtggga
    39961 tacgccgtgg cagccgattc gtgaatcagc cgcactgctg cgctactggc gtgcggacct
    40021 ggcccgcgag gcgctgttct ggcatgttca gcaagcgtta agcgccagtg acagcaaaga
    40081 catcggtctt ggatttgact gccgtgtgct gtatctgcgt gcgcagtgtg ccatcaacat
    40141 cgaatcacca aacgacaagc tgaacagcaa ccttaatctg gtggcgcgtg aactggcgaa
    40201 ggttcgcgat aaaggtctgc cggaagaaga gttcaatgcg ttagtggcgc aaaagaaact
    40261 ggagctgcag aaactgtttg ccgcctatgc acgggctgat accgatattc tgatgggcca
    40321 gcggatgcgt tcgttgcaaa atcaggtcgt cgatatcgcg ccggagcagt atcagaaact
    40381 gcggcaggat ttccttaaca gcctgactgt ggagatgtta aatcaggatc tgcgtcagca
    40441 gttgtcgaat gatatggcgt taatactgct gcagccgaaa ggcgagccgg aatttaacat
    40501 gaaagcgttg caggcggcct gggatcaaat catggcccca tctaccgccg ctgcgaccac
    40561 ctctgtcgcc acggatgacg tacatcctga agtgacggat attccacctg cacagtaatg
    40621 cctgatggtg atgagttagc acaaccatca ggtatttatg gatgagctgg atagaggggt
    40681 aacactttat ccagcctttt gcatatgctg cgtttacgct ggcatcgcct cacgcgggat
    40741 aatcgcgccg cgatactgaa taacggtact tgcggtcagg tgcccgcgtt tcgccgcgtt
    40801 ttccgcactg ccgcccgtca aacgtaccgc cagataaccg gcgctgaaag agtcgccagc
    40861 tgcggtggta tcgatcactt tttctttcgg cagtttcacc gccggaacat caactaacgc
    40921 ttcaccagca atggacacca ggcaagaatc cgccccgcgt ttcaccacca cttctttcac
    40981 gcccgcgtta tgggtgcgcg caatgacgtc ttccaccggc tgttgacccc acagcgcgtc
    41041 ttcgtcgtcc agcgtcagga aggcgatatc cgtgcattcc agcatttgct ggtacacctg
    41101 ctgtgtctct tctttgctgg cccacaggcg cggacgatag ttattgtcga aaatcacttt
    41161 tccgcctttg gcgcggcatt cgcgcagcag ggaaagcagc ttttcgcggc tggtcgggct
    41221 taagatcgcc aggctaatcc cgctcaggta gagataatcg aaattcgcca gctcttcgca
    41281 aatcgccgca gactgctcgc tcgccagcca gaatttggcg gcggcttcgt tacgccagta
    41341 gtagaacgta cgttcgccgg tgctgtcggt ttcaatgtag taaaggcccg gaagacggtt
    41401 ttccatccgt tgggtcaggg aagtatcaac gttctcgccg tgccaggcgt ccagcatctg
    41461 ctggctaaaa ctgtccgttc ccagcgccgt tacgtaatga acggttaatg ccgcaggatc
    41521 gacctgacgg gcgatataga cggaagtgtt cagggtatcg ccgccgaaac cgcgcttaac
    41581 gtccgcgcct ttctcggaaa gctcaatcat gcattcgcca atcacggcaa tctttttgga
    41641 catagtcgtg aacctgatct gtaaaaaatt agcgttagtt tgcgctgtgg tggtttgctg
    41701 gtcaaccata ttaaaacagt gttccattat ttttttgagc cagaacgtat tcctgaaaga
    41761 ttccgttgtg gagtgaggaa atttaccccg gccaggcggt aaagttctgc ccttacgcgc
    41821 cgataatctt tgtcgagtcc gggcagcatc acttttaaac acaggacatc tttgatgata
    41881 aggcaggtta tccagcgaat aagcaaccct gaagcaagca tcgagagctt gcaggaacgg
    41941 cgtttttggt tgcagtgtga gcgtgcttac acctggcagc cgatctatca aacatgcggg
    42001 cggttaatgg ccgtggagct attaacggtg gtcacgcatc ccttgaaccc ttcgcaacgc
    42061 ctgccgccgg atcgctattt tactgaaatc accgtcagcc atcggatgga ggttgtgaaa
    42121 gagcagattg atttgctggc gcaaaaagcc gacttctttg tagagcacgg cctgctggca
    42181 tcggtcaata ttgatggccc tacgctcatc gccctgcgtc agcaaccaaa aatcctgcgc
    42241 cagattgagc gtcttccctg gctgcgtttc gaactggtgg agcatatccg tctgccgaaa
    42301 gattcaacct ttgcctcgat gtgtgaattt ggcccgctgt ggctggatga ttttggtacc
    42361 gggatggcaa atttctctgc actaagtgaa gtgcgttatg actacatcaa aatcgcgcga
    42421 gaactgtttg tgatgctgcg tcagtcgccg gaaggccgca cactcttttc tcagctttta
    42481 catctaatga atcgctattg tcgcggggtg attgtcgagg gcgtagaaac accggaagag
    42541 tggcgtgatg ttcagaactc gcccgcattc gccgcacaag gctggtttct ttcacgcccg
    42601 gcaccgatgg aaacgctgaa tacggcggtt ctggcgctat aagctgcctc attttccgcc
    42661 gggctggact atctttagga ctggcacagg aagaatatga ggaagcgaac gatgagcaag
    42721 gcaggcaaaa taaccgctgc gatttcaggg gctttcttgt tgttgattgt cgtggcgatc
    42781 attttgattg caacttttga ctggaatcga ctcaaaccga ccatcaacca gaaagtctct
    42841 gcggagttga atcgtccgtt cgctatccgt ggcaatctgg gcgtggtgtg ggagcggcaa
    42901 aaacaagaaa ctggctggcg cagctgggtg ccgtggcctc atgtacatgc agaagacatc
    42961 attcttggta atccaccgga tattcccgaa gtcacgatgg tgcatttgcc gcgagtagag
    43021 gcaacgctgg caccgctggc gctgctgacc aaaacggtct ggctgccgtg gatcaagctc
    43081 gaaaagcccg acgcgcgcct gattcgcctc tctgaaaaga acaataactg gacgtttaat
    43141 cttgccaacg atgacaacaa agacgcgaat gcaaagccgt cggcatggtc gtttcggctg
    43201 gataatattc ttttcgatca agggcggatc gccattgatg acaaagtaag caaagcggat
    43261 ctggaaattt ttgttgatcc gttaggcaag ccgctgccat tcagcgaagt tactggatcg
    43321 aaaggtaaag cggataaaga aaaggtgggc gattacattt ttggcctgaa ggcgcaggga
    43381 cgttataacg gtgaaccgct cacgggtacg ggaaaaatag gcggtatgct ggcgctgcgt
    43441 ggcgaaggga cgccgtttcc ggtacaggct gatttccgtt caggtaatac ccgtgttgct
    43501 tttgatggcg tcgtgaatga cccaatgaag atgggcggtg tcgatttacg gcttaaattt
    43561 tctggcgatt cactgggtga tctctatgaa ctgacgggcg ttctgctgcc cgataccccg
    43621 ccgtttgaaa cggatggtcg gctggtagcg aaaatcgaca ctgaaaaatc gtcggtcttt
    43681 gattatcgcg gttttaatgg gcgaattggc gatagcgata tccacggttc tctggtctac
    43741 accaccggaa agccacgacc aaaactggaa ggtgatgtcg agtcgcggca attgcggctg
    43801 gcggacctgg gaccgttgat tggcgttgat tccgggaaag gggcagaaaa gtcgaaacgg
    43861 tctgaacaga agaagggcga aaaaagcgtt cagcctgcgg gcaaagtgct gccttatgac
    43921 cgcttcgaaa ccgataaatg ggacgttatg gatgccgatg ttcgcttcaa agggcggcgc
    43981 attgagcatg gcagtagcct gccgattagc gatctttcta ctcatatcat cctcaaaaat
    44041 gctgacctgc gcctgcaacc gctgaaattt ggcatggcgg gcggcagcat tgcggcgaat
    44101 attcatctgg aaggcgataa aaagccgatg caggggcggg cagatattca ggctcgtcga
    44161 ctgaaactga aagaactgat gcccgatgtg gaactgatgc agaagacgct gggggaaatg
    44221 aacggtgacg cggaactacg cggtagcggt aactcggtgg cggcgctttt aggcaacagt
    44281 aacggcaacc tgaaactgtt gatgaatgac gggctggtga gccgcaacct gatggagatt
    44341 gttgggctga atgtcggcaa ctacattgtc ggtgcgatat ttggtgacga tgaggtgcgg
    44401 gtgaactgcg cggcggcgaa tctgaatatt gccaacggcg tggcgcgccc gcagattttt
    44461 gctttcgata ctgagaacgc gttgattaat gttaccggca cggcaagttt tgcttcggaa
    44521 cagctggatt tgactattga tccggagagt aaagggattc ggattatcac actgcgttcg
    44581 ccgctgtatg tgcgtgggac gtttaaaaat ccgcaggctg gggtgaaagc cggaccgctg
    44641 attgcccgtg gtgctgttgc tgcggcactg gcaacgctgg taacaccggc ggcggcgtta
    44701 ctggcactga tctcaccttc cgaaggggag gctaatcagt gtcggacgat attgtcgcag
    44761 atgaagaagt gatgataatt gccggatgtc tgacatccgg caattaaaca gatttagatt
    44821 tttggcaaag tgtgctttgt tcatgccgga tgcggcgtga acgccttatc cggcctacaa
    44881 atccttgtga attcaatata ttgtatggaa attcagtcct gataagcgta gcgcatcagg
    44941 cttttcacac ttattacaac gactgatgtc gcgtctcatg ggtcagcagc agggcgatta
    45001 acgtcaagcc agccatcgcc gccagatata accccaccgc acctaacccg tagttagcct
    45061 gcaaccaggc tgcgatatac ggcgcaacgg aagccccgag aatcgacgat acgttgtaag
    45121 agaacgatgc tccggtgtaa cgcacttctg tcggaaacag ctctggtaac agcgcaccca
    45181 tcggcccgaa ggtcagcccc atcagactta accccagcag caggaaggca aaaaccagaa
    45241 tcgggttgcc agaaccgagc agcgggttaa aggcgaacag cgcgaacagg atgatcagcg
    45301 tggtgatgat caccatgctt ttacggcgac caaaggcatc agccagtaat ccggcgactg
    45361 gcaccatcac gccaaaacca ataactgcca tcatcagcat ccacaacact tcgttacgcg
    45421 gcaggccaag cccaactggc gcggcggcgg tactgaaggt catagagtag accgtcatga
    45481 tgtaaaacag cgtataggtt gccagcataa tgaacgtacc cagtacggtt acgcgaacat
    45541 gtttggtcag cagcgtaccc agcgggatct tcacctgttt tttcgcttta gcgactttct
    45601 caaacaccgg cgactcatgc agcgacacgc gaacatacag gccgataatg accagcaccg
    45661 ccgagaagat aaatggcaca cgccagcccc agctcataaa ctgctcatcg gtcagtagcc
    45721 aggaaagcag caagaaagtg ccgttggcaa agaagaagcc aatcggtgcg cctagctgtg
    45781 ggaaggagcc atacagtgca cgtttgcgcg gtggggcgtt ttcagtcgcc agcagcgccg
    45841 caccgcccca ttcaccgcct aagcccagac cctgaccaaa tcgagccagc gccagcagca
    45901 gtggggcgaa aataccaatc gtggcatagc ccggcagcag accaatcacc acggtcgaaa
    45961 tccccatcgt tagcaacgag gcgaccagcg tcgctttacg cccaacgcga tcgccaaaat
    46021 gaccaaaaac ggcagagcca atggggcgcg cgacgaaggc gatggcgaag gtggcgagcg
    46081 actgtagcgt tgctgccgtt ggatcgccct gcgggaagaa gatatgcgga aacacaataa
    46141 cggccgcagt ggcgtaaatg taaaagtcga agaactcaat ggccgtgcca atgagagagg
    46201 cgacaaggac tttattacgc gagttgatcg gcgtgtattc ttgttcgtgg tcgagtgttg
    46261 tggctgttgc ttgcataatc ttttcttatt ttttggctaa cgaatagcca tattacgcac
    46321 agcaaaagtg atatttcaat gtgtaacaaa tcgcggaaat ggtcaaaaac caggcaaaaa
    46381 ggggataatg tttaggccag tgatttttct tctatgaaat gcttcacata attttataaa
    46441 tagtggataa aactggtttc aggttaaata aaagttacca actggattta tatgctgggg
    46501 ttatgagttg gagtgaaatt gtttgctccc aaatagatgg ctgaagaggc tctggggaag
    46561 agtttcaaga ggttatgtgc gcattatagc cggatgcgat gctggcgcat cttatccggc
    46621 ctacggggag ccaggccgaa taaaatcgta tgccggataa ggcgtttacg ccgcatccgg
    46681 cctattttaa ggctgcgttt tccctggcat tcgcgggtcg tctttatatt cggcggtggc
    46741 aatccacgcc gcgcaaaaca gcgtcaaacg ggcgaagaag tagaaaaatg ccatcagccc
    46801 cagcacggaa ccaaatgctg cgccagaggg ggatttcatc agcgatggca gggtgtaggt
    46861 catcacgatt ttaatcactt caaaaccaat agcagccaga aatgttccgc gaatcagggc
    46921 ttttttgcgc ggacggtggc gcggcaaccg ccagaagatc cagaagaaaa gcagatagtt
    46981 ggcgaagatg gaaatcgcca ggccaatcaa tcgccacgtc ggtttcagcc actcaatgct
    47041 gttcaggtgc agggcgctaa taatcatttg ctgcgccgaa ccggcaaccg aggtgatcga
    47101 aagcgtcaca atcagcgcaa tcaacaaacc aatcagtgaa ataaaatcac gcagatattt
    47161 tacccagaac ttctcctgat cttgcggcga gcgttcccag acatcgcgcg actgggcacg
    47221 aatcgcttca cgcaggttac ccatccagtt gatgccggaa taaagtgcca ccgccaggcc
    47281 gacaagccct acagtcgtac gctgctgaac ggcggtgttg atggtgtttt tcaacgtggc
    47341 ggctagtgtc ggatcgctga tgttttgcag aattttgtcg aagatatcct gtagcaacat
    47401 cggatgggag gccagcacaa agccccctgc ggcaaacgac accatcaaaa tcgggatcat
    47461 cgacaaaaat gagaaatagg tgatagccgc accaaactgg ttacccagcc gatcgttaaa
    47521 gcgttctgtc gcgcgaataa ggtgcgcaat aaccggttgg cgttggactt tttcggcagt
    47581 ggtggtgacg gtttccagcg cctggctaac tttgccgcct ttctcgctat tatccaactg
    47641 cttaatcggc tcgtgctcca gatcctgggt gggacgtttg atctcgtttt cctgcgtcat
    47701 ccgctctttt gtccttctgt cgtggtcatt cttaaaaagt atagtcggtc agtccaggtg
    47761 ctctttcatt acgccagcca gccactgcat aaacaggttt acacgccggg aaagctcccg
    47821 acgctgcggg taaaccagcg aaagggagag cggctcggca cggtagcctg gcaacacttc
    47881 aataagccgc ccggcacgca gggcttcgcg cacggcaatg cgcgggatct gaataatccc
    47941 cagaccggca agaccggcgg cgagatagtt ttcgctactg tttaccgtca acatgccgcc
    48001 agacttaaac cactggacgc cattaacgct ggcaacctca aaacctaacg gatgtacacc
    48061 caggtgcggt gtataacgca ctattgcgtg tgaagtcaga tcatcggggc tttgcggata
    48121 accaaagcgc gtcagatagt gcggactggc acagttgacc atggttagtt tgccgagggg
    48181 acgggcgata acgccatctt ccggtaacgc gccagtgcgt atcacgcaat caaaaccatc
    48241 atgaagaata tctaccggac ggttatggct actcagttcc agctcaattc ccggatgctg
    48301 atagagaaat tccgacagac gcggcagtaa caggtttttc gcgattcctg acgggatgtc
    48361 gatgcgtaat ttaccgctga tactggtggc atcctgttga aacagaccgt ccagttcgct
    48421 gagattactc aacacatctt ttgcccgttg ataataggtc atgccttctg gcgtgagttt
    48481 gacccgtcgc gtagtgcgct ggagaagctg ggtgccaagt tgatgttcca gtgcctgtat
    48541 ctggcgcgaa acacttccct ttggcaaagc aaagaaatcc gccgcgcggg aaaaactttc
    48601 cagctccgcg actttgatga acaactgcat tgcgtgaatt ttatccattt ctttgaccga
    48661 ttgttgttca cagcgaaaca gtaacgcgca tatcgcattg tttattgttt tttctaacat
    48721 ctaatgccgg gtttcacaat gttgtcatga cgaatgtggc gatttctcag caggaaaagg
    48781 gcggaatagc tttatgagaa aggtgaatcc ccttaaacag agcgtatttt tcagtgattc
    48841 cctttaaagt gaatagagga cgtattggct atttgtgctt atttttgtct cattgccatt
    48901 tgttattttg gcttgtagtc gcggaatttc atttttaatt gcatgcgtac taaccaccaa
    48961 tataaggaaa tcctcaaaat tgttttaaat agtgccctcg acatgtaatt tcaggcgttt
    49021 agctgtaact atttatatcg gtaactattt tctacgcgtc atctctgtag aatggccacc
    49081 gccaaatggc gaattcttat tttttctcgc gggtaatacc cttccactac ttaagtcttt
    49141 ttaacgtatt caggtggaga ttcagttatg caaatagtca tgtttgacag gcagtcaata
    49201 tttattcatg gaataaaaat cagtttacag cagcgtattc caggagtgag tattcagggg
    49261 gtcagtcagg cagacgagtt atggcaaaag ctggaaagtt accctgaagc cttagttatg
    49321 ctcgatggcg atcaggatgg tgagttttgc tactggttgc tgcaaaaaac cgtggtgcag
    49381 tttcctgagg ttaaggtgtt aatcacggcg acggattgca ataaacggtg gttacaggaa
    49441 gttatccatt ttaatgtgct ggccattgtg cctcgcgatt caaccgtcga gacatttgcg
    49501 ttggcggtaa atagtgcggc gatggggatg atgtttctgc ctggagactg gcggacgacg
    49561 ccggaaaagg acattaaaga tctcaaatcg ttaagcgccc gccagcggga gattttaacc
    49621 atgttagcgg cgggcgaatc aaataaggag atcggcagag cgctgaatat cagtaccgga
    49681 acggtaaaag cgcatctgga atccctgtat cgccgtctgg aagtgaaaaa tcgcacccag
    49741 gcggcaatga tgttaaatat ctcctcctg
//
LOCUS       DAEEUE010000020        49536 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.020, whole genome shotgun sequence.
ACCESSION   DAEEUE010000020 DAEEUE010000000
VERSION     DAEEUE010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49536
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.020"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..1156)
                     /gene="setB"
                     /locus_tag="KLK84_001219"
     CDS             complement(<1..1156)
                     /gene="setB"
                     /locus_tag="KLK84_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HBE2008620.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVLLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLF"
     gene            1524..2654
                     /gene="fruB"
                     /locus_tag="KLK84_001220"
     CDS             1524..2654
                     /gene="fruB"
                     /locus_tag="KLK84_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HBE2008621.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2654..3592
                     /gene="fruK"
                     /locus_tag="KLK84_001221"
     CDS             2654..3592
                     /gene="fruK"
                     /locus_tag="KLK84_001221"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBE2008622.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3609..5300
                     /gene="fruA"
                     /locus_tag="KLK84_001222"
     CDS             3609..5300
                     /gene="fruA"
                     /locus_tag="KLK84_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HBE2008623.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5724..6665
                     /locus_tag="KLK84_001223"
     CDS             5724..6665
                     /locus_tag="KLK84_001223"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HBE2008624.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSARKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENTECLN"
     gene            6653..7591
                     /gene="psuG"
                     /locus_tag="KLK84_001224"
     CDS             6653..7591
                     /gene="psuG"
                     /locus_tag="KLK84_001224"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HBE2008625.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7685..8935
                     /locus_tag="KLK84_001225"
     CDS             7685..8935
                     /locus_tag="KLK84_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBE2008626.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9006..9700)
                     /gene="yeiL"
                     /locus_tag="KLK84_001226"
                     /pseudo
     CDS             complement(9006..9700)
                     /gene="yeiL"
                     /locus_tag="KLK84_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9831..10772
                     /gene="rihB"
                     /locus_tag="KLK84_001227"
                     /pseudo
     CDS             9831..10772
                     /gene="rihB"
                     /locus_tag="KLK84_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
     gene            10872..12122
                     /locus_tag="KLK84_001228"
     CDS             10872..12122
                     /locus_tag="KLK84_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBE2008627.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12178..13266)
                     /locus_tag="KLK84_001229"
     CDS             complement(12178..13266)
                     /locus_tag="KLK84_001229"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBE2008628.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13269..14126)
                     /gene="nfo"
                     /locus_tag="KLK84_001230"
     CDS             complement(13269..14126)
                     /gene="nfo"
                     /locus_tag="KLK84_001230"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBE2008629.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLHTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14200..15249)
                     /locus_tag="KLK84_001231"
     CDS             complement(14200..15249)
                     /locus_tag="KLK84_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HBE2008630.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15348..16229
                     /locus_tag="KLK84_001232"
     CDS             15348..16229
                     /locus_tag="KLK84_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE2008631.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DEQVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16435..17904
                     /gene="lysP"
                     /locus_tag="KLK84_001233"
     CDS             16435..17904
                     /gene="lysP"
                     /locus_tag="KLK84_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HBE2008632.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18196..20187
                     /gene="cirA"
                     /locus_tag="KLK84_001234"
     CDS             18196..20187
                     /gene="cirA"
                     /locus_tag="KLK84_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HBE2008633.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YCVSHNGRWDYGTSELKSYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYMVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGGESSVIVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYSVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(20219..21054)
                     /gene="yeiG"
                     /locus_tag="KLK84_001235"
                     /pseudo
     CDS             complement(20219..21054)
                     /gene="yeiG"
                     /locus_tag="KLK84_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21312..21980
                     /gene="folE"
                     /locus_tag="KLK84_001236"
     CDS             21312..21980
                     /gene="folE"
                     /locus_tag="KLK84_001236"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBE2008634.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21997..23073
                     /locus_tag="KLK84_001237"
     CDS             21997..23073
                     /locus_tag="KLK84_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HBE2008635.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRLWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23215..24255
                     /gene="galS"
                     /locus_tag="KLK84_001238"
     CDS             23215..24255
                     /gene="galS"
                     /locus_tag="KLK84_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HBE2008636.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24535..25533
                     /gene="mglB"
                     /locus_tag="KLK84_001239"
     CDS             24535..25533
                     /gene="mglB"
                     /locus_tag="KLK84_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HBE2008637.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25594..27114
                     /gene="mglA"
                     /locus_tag="KLK84_001240"
     CDS             25594..27114
                     /gene="mglA"
                     /locus_tag="KLK84_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HBE2008638.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27130..28140
                     /gene="mglC"
                     /locus_tag="KLK84_001241"
     CDS             27130..28140
                     /gene="mglC"
                     /locus_tag="KLK84_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HBE2008639.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28176..29389)
                     /gene="preA"
                     /locus_tag="KLK84_001242"
                     /pseudo
     CDS             complement(28176..29389)
                     /gene="preA"
                     /locus_tag="KLK84_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29383..30621)
                     /gene="preT"
                     /locus_tag="KLK84_001243"
     CDS             complement(29383..30621)
                     /gene="preT"
                     /locus_tag="KLK84_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HBE2008640.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30815..31054)
                     /locus_tag="KLK84_001244"
     CDS             complement(30815..31054)
                     /locus_tag="KLK84_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HBE2008641.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31057..31776)
                     /gene="sanA"
                     /locus_tag="KLK84_001245"
     CDS             complement(31057..31776)
                     /gene="sanA"
                     /locus_tag="KLK84_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HBE2008642.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31926..32810)
                     /gene="cdd"
                     /locus_tag="KLK84_001246"
     CDS             complement(31926..32810)
                     /gene="cdd"
                     /locus_tag="KLK84_001246"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBE2008643.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32940..33635)
                     /locus_tag="KLK84_001247"
     CDS             complement(32940..33635)
                     /locus_tag="KLK84_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HBE2008644.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33632..34030)
                     /locus_tag="KLK84_001248"
     CDS             complement(33632..34030)
                     /locus_tag="KLK84_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBE2008645.1"
                     /translation="MSKTLNIIWQYLRAFILIYACLYAGIFIASLLPVTIPGSIIGMQ
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34269..35216
                     /gene="dusC"
                     /locus_tag="KLK84_001249"
     CDS             34269..35216
                     /gene="dusC"
                     /locus_tag="KLK84_001249"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HBE2008646.1"
                     /translation="MRVLLAPMEGVLDSQVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISDC
                     DAVMIGRGAFNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35587..35670)
                     /gene="yohP"
                     /locus_tag="KLK84_001250"
     CDS             complement(35587..35670)
                     /gene="yohP"
                     /locus_tag="KLK84_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HBE2008647.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            35894..37330
                     /gene="mdtQ"
                     /locus_tag="KLK84_001251"
     CDS             35894..37330
                     /gene="mdtQ"
                     /locus_tag="KLK84_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HBE2008648.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVDGVETDINASKTRQQLNDVAGKMKIIEARLNALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIRLTGALGGGYKR"
     gene            37383..38144
                     /locus_tag="KLK84_001252"
                     /pseudo
     CDS             37383..38144
                     /locus_tag="KLK84_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38261..38839)
                     /locus_tag="KLK84_001253"
     CDS             complement(38261..38839)
                     /locus_tag="KLK84_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBE2008649.1"
                     /translation="MALNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39009..39596
                     /locus_tag="KLK84_001254"
     CDS             39009..39596
                     /locus_tag="KLK84_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HBE2008650.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39770..40702
                     /gene="pbpG"
                     /locus_tag="KLK84_001255"
     CDS             39770..40702
                     /gene="pbpG"
                     /locus_tag="KLK84_001255"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HBE2008651.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40740..42455)
                     /gene="dld"
                     /locus_tag="KLK84_001256"
     CDS             complement(40740..42455)
                     /gene="dld"
                     /locus_tag="KLK84_001256"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBE2008652.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42651..44948
                     /gene="bglX"
                     /locus_tag="KLK84_001257"
     CDS             42651..44948
                     /gene="bglX"
                     /locus_tag="KLK84_001257"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HBE2008653.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45200..46117
                     /gene="osmF"
                     /locus_tag="KLK84_001258"
     CDS             45200..46117
                     /gene="osmF"
                     /locus_tag="KLK84_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HBE2008654.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     FQVLESHGVPTVNKVQLGTTPVGRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46124..47281
                     /gene="yehY"
                     /locus_tag="KLK84_001259"
     CDS             46124..47281
                     /gene="yehY"
                     /locus_tag="KLK84_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HBE2008655.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALLVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47274..48200
                     /gene="yehX"
                     /locus_tag="KLK84_001260"
     CDS             47274..48200
                     /gene="yehX"
                     /locus_tag="KLK84_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HBE2008656.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48205..48936
                     /gene="yehW"
                     /locus_tag="KLK84_001261"
     CDS             48205..48936
                     /gene="yehW"
                     /locus_tag="KLK84_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HBE2008657.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(48917..49024)
                     /locus_tag="KLK84_001262"
     CDS             complement(48917..49024)
                     /locus_tag="KLK84_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HBE2008658.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(49084..49536)
                     /locus_tag="KLK84_001263"
                     /pseudo
     CDS             complement(49084..49536)
                     /locus_tag="KLK84_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT        11959 a        12201 c        13310 g        12066 t
ORIGIN      
        1 aaaacagagt ggcgataatc atcaccatcg caaaccagaa cacagcgtga taattccaga
       61 tctcggcgac gatgcccgcc actgatcctg cgatgatcca gcccacgcgc gacgtgttgg
      121 tatagagcgt ggtggctgaa cctgcctgac cgggcatcaa atcctgaaaa tagagcatcc
      181 cgatgccgcc cagaatgcca ataaaaatag catttagcag ctgcaagccc aacagaatgg
      241 caggtgaatg cgccatcagc attcctgcgt aaaaacagac gccacccacg gcagcaacgc
      301 gcattaagaa acgcttaccc agacgtttgg cgaaatatcc ggcaatcaac atcgtcggga
      361 tttccagccc ggcggcggtc cccatcatca caccggctag tttctcggga agatgcagtt
      421 cgttgataat aaatagcggc atgttgatga tgtacaggct gttcgagccc cacatcaatg
      481 tacaaatgac aaacagcagt agcgtatcgc gacggttacg gcgcggcgct tcaaccgtgc
      541 cggtcgccag cggaagctct ttttgcatcg acggtaaaaa cagccacacc atcacgccgc
      601 aaacaataaa cgctaccgct gcgctcagat acattaccgt aaagctgaaa cccatcgcta
      661 acgcataagc cagcggtggg ccaatgaccc atgccagtga aacctgagcg cgtaaaaaag
      721 agctgaacat caccgcctca cgtccggttt tgtcggcatg ttcacgggca agggcaaaca
      781 tttgcgggtt agcggtcgag ccaaagctgc taagaaggac gccaacgaat agcaaaacaa
      841 agtagttgcg attccaagca aaaagggtgc aggccagcac gcctaacagg cagcaaaaga
      901 caatcagcga tttgcgatcg ccgcgcttat cagagcgccc agcgagaaac tgacttacca
      961 gaatcccaat gacagcgctg ccggtaaaga agaatcccac catcgccgga cgggcatgta
     1021 cttcatcggt aaggaaaata ctgagtgtcg gggtttgcag agcacccgca ataccggtga
     1081 gaaaggcaac gattaaaaac gccgtcgagg tcaggtcaaa cgatttcgcg ctggagactg
     1141 cgggggagtt atgcatgctg gtcgatcact ggaagaggaa gacgcggagt ttacgctgct
     1201 tgtcagaaaa taaacagaca gcgaaatcaa aagcgccacg aataatcaaa atgccatttc
     1261 actgttgctg attggctgct gacgctgctg aaatgaaatt gctgataacg gattttccca
     1321 tcagcaatta ggaaaaatga caaaaaattg tgcagcacat caaacttttg ctcataactt
     1381 tacggctttc cttgcgtgct gaaacagaag tattatgctt tcttgaaacg tttcagcgcg
     1441 atcttgtctt taaccctaag ctaggttggc gctttttttc tcatagaggc tgaatcgttt
     1501 caattcagca agagaggaga actatgttcc agttatccgt acaggacatc catccgggcg
     1561 aaaaggccgg agacaaagaa gaggcgattc gccaggtcgc tgcggcgctg gtgcaggccg
     1621 gtaatgtagc agaaggctac gtcaatggca tgctggcgcg cgaacagcaa acctcaacgt
     1681 tcctcggcaa tggtattgct attccacacg gcactaccga cacccgcgat caggtgctga
     1741 aaaccggcgt tcaggtattt cagttcccgg aaggcgtcac ctggggtgac ggtcaggtag
     1801 cgtacgtggc aatcggtatt gctgccagct cggataaaca tctgggcctg ctacgccagc
     1861 tgacccacgt actgagcgat gattccgttg ctgaacaact gaagtcagca acaacagcag
     1921 aagaacttcg cgcattactg atgggcgaaa agcagagtga gcagctgaag ctcgacaacg
     1981 aaatgctgac gctggatatc gtcgccagcg atctgctgac tcttcaggcg ctgaacgctg
     2041 cgcgtctgaa agaggcgagg gcagttgacg ccactttcgt caccaaagcc atcaatgaac
     2101 aaccgctgaa tctcggacag ggtatctggc tgagcgatag cgccgaaggc aatctgcgta
     2161 gtgcgattgc ggtaagccgt gcggcaaatg cttttgatgt ggacggcgaa acggcagcca
     2221 tgctggtgag tgtggcgatg aatgacgatc agcccatcgc ggttcttaag cgtctcgctg
     2281 atttgttgct cgacaataaa gctgaccgct tgctgaaagc ggatgcggca acgttgctgg
     2341 cgctgctgac cagcgatgat gcgccgaccg acgacgtgtt aagcgcggag tttgtagtgc
     2401 gcaatgaaca cggcctgcat gctcgtccag gtaccatgct ggtcaatacc attaaacaat
     2461 ttaacagtga tattaccgtg acaaaccttg atggcaccgg caaaccggca aacggacgta
     2521 gtctgatgaa agttgtggca cttggcgtta agaaaggtca tcgcctacgc tttaccgccc
     2581 agggtgcaga tgctgaacag gcgctgaaag ccattggcga cgctatcgct gctggtcttg
     2641 gggagggcgc ataatgagca gacgtgttgc tacgatcacc cttaatccgg cttatgacct
     2701 tgttggtttc tgcccggaaa ttgaacgcgg cgaagtgaac ctggtgaaaa ccaccggtct
     2761 gcatgcggcg ggtaaaggca tcaacgtggc caaagtatta aaagacctgg gaattgatgt
     2821 caccgttggc ggcttcctgg gtaaagacaa tcaggatggt tttcagcaac tgttcagcga
     2881 gctgggcatt gccaaccgtt tccaggttgt acaggggcgc acccgaatta acgttaagct
     2941 gacggaaaaa gacggcgaag tgaccgactt caacttctcg ggtttcgaag tcacccccgc
     3001 cgactgggaa cgctttgtga ctgattctct gagctggctc ggtcagttcg atatggtctg
     3061 tgtcagcgga agcttaccgt caggcgtcag cccggaagcg ttcaccgact ggatgactcg
     3121 cctgcgtagt cagtgtcctt gcattatctt tgatagtagc cgtgaagcgt tagtagcagg
     3181 tttgaaagcg gcaccgtggc tggtgaaacc taaccgccgc gagttggaaa tctgggcagg
     3241 ccgtaaactg cctgaaatga aagatgtgat tgaagctgca catgcgctac gtgaacaagg
     3301 tattgcgcat gttgttattt cactgggtgc cgaaggcgcg ctttgggtta atgcctccgg
     3361 cgaatggatc gctaaaccac cgtcagtcga tgtcgtaagc accgttggcg caggggattc
     3421 tatggttggt ggcctgattt atggcttgct gatgcgtgaa tccagtgaac acacactgcg
     3481 tctggcgaca gctgttgcag ccctggcggt aagtcaaagc aatgtgggta ttaccgatcg
     3541 tccgcagttg gccgcaatga tggcgcgcgt cgacttacaa ccttttaact gacagcagga
     3601 gaggcataat gaaaacgctg ctgattattg acgctaatct cggtcaggca cgcgcctata
     3661 tggcgaagac cctgctgggc gcggcggcgc gaaaagcaaa actggaaatc atcgacaatc
     3721 cgaacgacgc agaaatggcg attgttctcg gtgattccat cccgaatgac agcgcgctga
     3781 acggtaaaaa tgtctggctg ggtgatattt cccgggcagt tgcgcaccct gagctgttcc
     3841 tgagtgaagc caaaggccat gcgaaacctt acactgcgcc ggtcactgcg acagcaccgg
     3901 ttgccgccag cggtccgaaa cgcgtagttg cggtgactgc ttgcccgact ggcgtagcac
     3961 acacctttat ggcggctgaa gccattgaaa ccgaagcgaa aaaacgtggc tggtgggtga
     4021 aagttgaaac ccgtggttct gttggcgcgg gtaatgcaat cactcccgag gaagtagccg
     4081 cagcggatct ggtgattgtg gcggcagata tcgaagtgga tctggcgaaa tttgctggta
     4141 aaccgatgta tcgtacctct accggtctgg cgctgaagaa aacctcgcag gaactggata
     4201 aagcggttgc tgaagcaacg ccgtatgaac cggcgggcaa agctcaaacg gcgaccactg
     4261 aaggtaagaa agagagtgca ggcgcttatc gtcacttgct aacgggcgtc tcttacatgc
     4321 tgccgatggt cgttgctggt ggtctgtgta tcgcgctttc ttttgctttt ggtatcgaag
     4381 cgtttaaaga gccgggtacg ttggcagcgg cgctgatgca gattggtggt ggttcagcct
     4441 ttgcgctgat ggtgccggta ctggcaggtt atattgcctt ctccattgcc gatcgtccgg
     4501 gcctcacgcc gggtctgatt ggcggtatgc tggcggtcag caccggttct ggcttcattg
     4561 gcggtattat tgcgggcttc ctggctggtt acattgcgaa gttaatcagt acgcaactga
     4621 aactgccgca gagtatggag gcgctgaaac cgatcctgat cattccgcta atttccagtc
     4681 tggtggtcgg tctggcgatg atctacctga tcggtaaacc agttgctggc attctcgaag
     4741 ggctgactca ctggctgcag accatgggga ctgcgaatgc ggttctgctg ggggcgatcc
     4801 tcggtggcat gatgtgtact gacatgggcg gtccggtaaa caaagcagcg tacgcattcg
     4861 gtgtgggtct gctgagtact caaacctatg gcccgatggc ggcgattatg gcggcaggta
     4921 tggtgccacc gctggcaatg ggcctggcaa caatggtggc gcgtcgcaaa ttcgacaaag
     4981 cgcagcagga aggtggcaaa gccgctctgg tattgggact gtgcttcatt tcggaaggtg
     5041 caattccgtt tgctgctcgt gatccgatgc gtgtgctgcc gtgctgtatc gtgggtgggg
     5101 cgctgactgg cgcaatctca atggcgattg gtgcgaaact gatggcaccg cacggtggtc
     5161 tgtttgttct gctgatccct ggcgctatta cgccggtatt gggttacctg gtagcaatta
     5221 ttgccggtac gctggtggcg ggtttggcct atgccttcct gaaacgtccg gaagtggacg
     5281 cagtagcgaa agcagcgtaa taaaaggtgt gttacagggc agaaatttcc tgccctgtaa
     5341 catttgatga gcgcaaagag aatgaccagc ttgtgaatgt aattttatga atttaaatgg
     5401 ttttatcttt cagtagaaac ttcaaacgtt catcaaacac taaggttatt tcctcaaggg
     5461 cagattattt tctgcccttt tttagtgttc ctgttctgtg catcccgtca caaattcctc
     5521 gcttttgtaa ttcaggcatt taatcgcatt ttgtgagcct gctcaaatct gcccattttg
     5581 cctctctggc ggtaatttac tttacgtaaa acatgcatac tatgagcaca tgtttaaata
     5641 tgaacacatg tttaaaccgt ctttgcagta tctatattct tgtggattga cagggttggt
     5701 aattttttac cagaggacgt gctatgcgcg aaaaggatta tgtcgtaatt ataggttcgg
     5761 cgaatattga tgtcgccgga tattcacatg aatcattaaa ttatgcggat tcaaatccag
     5821 gtaaaataaa atttacgcct ggtggagtag ggcgcaatat tgcacaaaac ctggcgttgc
     5881 tgggtaacaa agcctggcta ctgagcgccg taggcagtga tttttatggt caatcgctgc
     5941 taacgcaaac caatcaatct ggtgtttatg tcgataaatg cctgattgtg ccgggagaaa
     6001 atacgtcgag ttatttatca ttactcgata ataccggtga aatgctggtt gctataaatg
     6061 acatgaatat tagcaacgct attacagctg aatatctcgc acagcaccgt gaatttattc
     6121 agagggcaaa ggtcattgtc gcggactgta atatcagtga agaggcactg gcatggattc
     6181 tggataatgc cgccaacgta cccgtatttg tcgatccggt ttcagcgcgg aaatgtgtca
     6241 aagtgcgcga ccgtctaaat cagatccaca ctctgaagcc aaaccgcctt gaagcggaaa
     6301 ccctgagtgg gattgcgctg tcagggcgtg acgatgtggc aaaagttgct gcctggttcc
     6361 atcaacatgg cctgaaccga ctggtattga gcatgggcgg cgacggcgtt tattacagcg
     6421 atatcagcgg tgaaaatggc tggtctgcgc cgatcaaaac caatgttatt aatgttaccg
     6481 gagcgggcga tgccatgatg gcgggacttg cttcgtgttg ggtagacgga atgccgtttg
     6541 ccgaatctgt tcgtttcgca cagggatgtt cgtcaatggc gctctcctgt gaatacacca
     6601 ataaccccga tttatcgatt gccaacgtta tatcgttagt ggagaacaca gaatgtctga
     6661 attaaaaatc tcccctgaat tattacaaat ttctccggaa gtgcaggacg ctttaaaaaa
     6721 caaaaaaccg gttgtggcgc tggaatcgac cattatttct cacgggatgc cgttcccaca
     6781 aaatgcccag accgcaattg aagtagaaga aactattcgt aaacagggcg cagtacctgc
     6841 cactatcgcc attattggcg gcgtgatgaa agtgggttta agcaaagaaa aaattgaatt
     6901 actgggtcgt gaagggcata acgtgaccaa agttagtcgt cgcgatttac cttttgttgt
     6961 tgccgccgga aaaaatggcg caaccactgt ggcttcaacg atgattattg cggcgcttgc
     7021 cggaattaaa gtatttgcca ccgggggaat tggtggtgtg catcgcgggg cggaacatac
     7081 cttcgatatt tctgccgatt tgcaagaact ggcaaatact aatgtcaccg ttgtttgtgc
     7141 cggggcgaaa tctattctcg atttaggatt aaccactgag tatttagaaa ccttcggtgt
     7201 gccgttaatt ggctatcaga ctaaagcgct gcctgcgttt ttctgccgta ccagctcgtt
     7261 tgacgtcagc attcgtctcg acagcgccag tgaaattgcc cgtgcaatgg cggtgaaatg
     7321 gcaaagcggg ctgaacggtg gcctcgtggt agcgaacccg atcccggaac agtttgcgat
     7381 gccggaggaa tctatcaatg cagccataga tcaagccgtc gccgaagccg aagagcaggg
     7441 cgttattggt aaagaaagta caccgttcct gctggcgcgc gttgctgagc tgaccggcgg
     7501 tgacagcctg aaatccaata tccagctggt gttcaacaac gccattctgg cgagtgaaat
     7561 tgccaaagaa taccagcgtc tcgcgggtta atttctgacg ggcagaaagc cgtcgcccgt
     7621 actcatcaag cataccaaaa cctggcgctg acgccgggtt tactggcatg aagggaaaat
     7681 cattatggat ataatgagaa gtgttgtggg gatggtggtg ttactggcaa tagcatttct
     7741 gttgtcagtg aataaaaaga gcatcagttt gcgcacggtt ggagccgcac tgctgctgca
     7801 aatcgctatt ggtggcatca tgctctactt cccaccggga aaatgggcag tagaacaggc
     7861 ggcattaggc gttcataaag tgatgtctta cagtgatgcc ggtagcgcct tcatttttgg
     7921 ttcgctggtt gggccgaaaa tggatgtcct gtttgacggt gcgggtttta tcttcgcctt
     7981 tcgcgtactt ccggcgatta ttttcgttac tgcgctcatc agtctgctgt actacattgg
     8041 cgtgatgggg ctgctgattc gcatccttgg cagcattttc cagaaagccc tcaacatcag
     8101 caaaatcgaa tcttttgttg cggttaccac tattttcctc gggcaaaatg agatcccggc
     8161 gatcgttaaa ccgtttatcg atcgcgtgaa tcgcaacgag ttgtttaccg caatttgtag
     8221 cgggatggcg tccattgctg gttcggtgat gattggttat gccggaatgg gcgtaccaat
     8281 tgactacctg ttagcggcat cgctgatggc gatccccggc ggtattttgt ttgcacgtat
     8341 tcttagcccg gcaaccgagc cttcgcaggt cacatttgaa aatctgtcgt tcagcgaaac
     8401 gccgccaaaa agctttatcg aagcggcggc gagcggtgcg atgaccgggc tgaaaatcgc
     8461 cgctggtgta gcgacggtgg taatggcgtt tgtcgcaatt attgcgctga tcaacggcat
     8521 tatcggcgga attggcggct ggtttggttt cgccaatgcc tctctggaaa gtatttttgg
     8581 ctatgtgctg gcaccgctgg cgtggatcat gggtgtggac tggagtgatg ccaaccttgc
     8641 tggtagcctg attgggcaga aactggcgat taacgaattc gtcgcttacc tgagtttctc
     8701 cccatacctg caaacgggcg gcacgctgga agtgaaaacc gtggcgatta tatcctttgc
     8761 gctttgtggt tttgctaact ttggttctat cggtgttgtc gttggcgcat tttcggctat
     8821 ttcgccaaaa cgtgctccag aaatcgccca gcttggttta cgggcgctgg cagcagcaac
     8881 gctttccaac ctgatgagtg cgactattgc cgggttcttt attggtctgg cgtaatgctc
     8941 gatttgtaga ccggataaaa cgtattaagc gttgcatccg gcaatcgcgc tattgcaaat
     9001 aatttttact gcatcatccc ggagaattta ttctccgggt ccatctccag cgccagtcct
     9061 gacaactgct ttctgttttt aattagatat cctttctttt aattaataaa ccgtcgtgaa
     9121 tgaattgcgc gagaacatat aaaagatgtc gataagaaac gcctaaatac tctgccgctt
     9181 gcgtatgctt ttcgtgataa agatcacctt cttgtgagag taatataaat gctgccaggc
     9241 gattcactaa cggaaatgat tgattctgag ttaaagaaac aatattacga taatttttat
     9301 gacttaaggt gacgcaaagt tttcgtaaaa atagggtgtc gtttaataac agcggacggt
     9361 aatgtttcat agggagcgca aggcaccaac actcttcaat agcctgcacc gcacgcggtt
     9421 catggtcttt gtcgattaac tcaatctcgc caataaaaca gggagcggca aagaaatcga
     9481 tcagcgacac gcgaccattg gcaagcgtgg cgtaaagcct ggcgcggcct cgcgtcagat
     9541 aaaacagcca tgacggctgt tgcccttcct gcacaatgta gtcacgcgct aaaaaatgaa
     9601 acagccgcgt atcggctgaa acatccgttg aaaagcaatc cttaaacgca gattcactca
     9661 tcaattgctg cttaaggtca ttattgtgga tttctttcac gatgtttatc cgctatgaga
     9721 tttctcatat ttagcatacg tacgctgttg ttaagattca atcattggcc cggtcgaatt
     9781 ttctttgctg tagaaaaaaa taacgcaact ggaaacagag gaaataaaca atggaaaaga
     9841 gaaaaattat tctggattgt gagccgggtc atgatgatgc tattgcaatg atgatggcag
     9901 cgaaacatcc ggcaatagat ttattaggca tcactattgt agcgggtaat cagacgcttg
     9961 ataaaacatt aattaatggc ctgaatgttt gccagaaact ggagattaat gttccggttt
    10021 atgcggggat gccgcaaccc attatgcgta aacaaatcgt tgccgataat attcacggtg
    10081 aaaccggact ggatggcccg gtattcgagc cgctgacccg ctaggcagaa agtacccatg
    10141 cggtgaaata tatcatcgat accctgatgg caagcgatgg cgatatcact ctggtgccgg
    10201 ttggtccgct ttcaaatatc gcggtggcaa tgcgtatgca acccgcaatc ctgcccaaaa
    10261 tccgtgaaat tgtgctaatg ggcggcgctt acggtacagg caacttcacc ccatctgccg
    10321 agttcaatat ctttgccgac ccggaagccg cccgcgtggt gttcacctcc ggcgttccat
    10381 tagtgatgat gggcctcgat cttaccaacc agaccgtttg caccccggat gtaattgctc
    10441 ggatggaaag ggtaggcggc cctgccggag agctgttcag cgacatcatg aacttcactc
    10501 tcaaaacgca gttcgaatac tacggcctgg ctggcggccc ggtgcatgac gccacctgca
    10561 ttggttatct gatcaaccct gatggcatta aaacccagga tatgtacgtc gaagtggacg
    10621 tcaacagcgg cccttgctac gggcgtacgg tctgcgacga actgggcgtt cttggcaagc
    10681 ccgctaatac caaagtcggc atcactattg ataccgactg gttctggggc ttagtcgaag
    10741 agtgcgtgcg cggctacatc aaaacccatt aagtgttgat aacaagccgg gacagtatcg
    10801 caagatgcag gcccggctat ttctgatggg cagaaagccg tcgcccaaaa tgacatgaag
    10861 agataatcac tatggatgtc atgagaagtg ttctgggaat ggtggtactg ctggcaattg
    10921 cgtttttact gtcagtaaac aagaagaaga tcagcctgcg taccgttggc gcggcgttag
    10981 tgttacaggt cgtgattggc gggattatgc tttggttacc gccagggcgt tgggtcgcag
    11041 aaaaagtcgc ttttggcgtc cacaaagtga tggcgtacag cgacgcaggt agcgcattta
    11101 tcttcggttc tctggtcgga ccgaaaatgg ataccttatt tgatggcgca ggatttatct
    11161 ttggtttcag ggtattaccg gcaattatct tcgtcactgc actggtgagt attctctact
    11221 acatcggtgt gatggggatc ttaattcgca ttctcggcgg tatcttccag aaagctttaa
    11281 atatcagcaa gatcgagtca ttcgtcgcgg tcaccaccat tttcctcggg caaaacgaaa
    11341 ttccggcgat cgtcaaaccc tttatcgatc gtctgaatcg caatgaatta tttacagcga
    11401 tttgtagtgg catggcctcg attgctggtt cgacaatgat tggttacgcc gcactgggcg
    11461 tacctgtgga atatttgctg gcggcatcgt taatggcgat ccctggcggg atcttgtttg
    11521 cccgcctgtt aagcccggct acggaatctt cgcaggtttc ttttaataac ctctctttca
    11581 ccgaaacacc gccaaaaagc attattgaag ccgatgcgac aggggcaatg accgggctga
    11641 aaatcgccgc cggtgtagcg acagttgtta tggcatttgt cgccatcatt gcgttaatta
    11701 acggtattat cggcggcgtt ggcggctggt ttggttttga acatgcctcg ctggagtcca
    11761 ttttaggtta cctgctggcc ccacttgcgt gggtaatggg tgttgactgg agtgatgcaa
    11821 atcttgccgg gagtttgatt ggacagaaac tggcaataaa tgaatttgtc gcttatctca
    11881 atttctcacc ctatctgcaa acggctggca ctctggatgc taaaaccgtg gcgattattt
    11941 ccttcgcgtt gtgcggtttc gctaactttg gttctatcgg ggtggtggtg ggggcgtttt
    12001 ctgcggttgc gccacaccgt gcgccggaaa tcgcccagct tggtttacgc gcgctggcgg
    12061 cggcgacact ttctaatctg atgagtgcca ccattgccgg gttctttatt ggtttagcgt
    12121 agtgtgcaat ttatgagccg gattagatgc ggtcaagcgt cgaatccggc aacattgtta
    12181 caccattggc actaatgaca gcgcgttatc ggcagacagg gtagggttgt ttaagctgcc
    12241 gctggcgcgt gaaattgccg cgcaggccat cgcaaaacgg gcgctgtcac ggaaattgtt
    12301 tccctccaga aagctatata ccagacccgc cataaaacca tcgtcagcac caaaactgtc
    12361 taccgtcgta tgcgctggca cagtcagcaa aaattgttca ccatcctttt cgctgcaata
    12421 aactgactca tcgggcaaat aaacaaacag ttgctgaaca ccttgctgat gcaatgcatt
    12481 cactgcggca ttacggtcag catcgctggt gatcgcctgt ccccataaaa tttccagctc
    12541 cggtaaagtg ggtttcaggg tgtgaatatg cgccagccag tgtttgattt tgcccgcttt
    12601 gaattctgaa acggtatcga caaacaccgg gatctcatca gcgagggtaa agacccattc
    12661 cagcgcctcg gctgtcaggt tgcaatcagc cagtaccacg cccgcatgac gaagtaaatc
    12721 gcgcgacccg ttcagtagtt gcggtgtcaa ctgttccagc agatgggtat cgttaattgc
    12781 cagcacggtt tgatcgtctc gattggcaat tgccagatac gtcgatgtgc tttgaccatg
    12841 caaacgaacg cagccggaga cattcacgcc ggcacggcgc gtttcttcca ggagcatttc
    12901 gccataaaag tcatcgccaa tcactgaaag caaatggacg tcacggccta acagcgccag
    12961 attgtgggcg atattgcgtc caacgccgcc tgccgaacaa tgaattgtac cgggatgaga
    13021 agccgcttgc gggtaatgga tatccgccat cccgcgaata tccatattga ttgtccccac
    13081 cactacgcag tattcctgct cggtgagaat gtaacctttg tctttaatcc ggcctttgcg
    13141 cattaaatcc ataatatgcg ccgcaacgcg cgaacgactg atttgcagca tgtccgcaat
    13201 ttcgttctgc tgaatcagcg ggttacgccg taaaattgca aggatctcct tttcccggtt
    13261 attcatcttc aggctaccgc tttttcagtt tgttgcgctt tcagccaggc gatctcttct
    13321 gcccagatat ccgggttgat ggtttcaagg atcagcggaa tgccgtcgaa acggtcgttc
    13381 tgcataatcc agcggaacgc atcatgaccg atattgcctt caccgaggct atgatggcgg
    13441 tcaacgcggc tgccaaaggt acttttcgca tcgttaaggt gcatcccgcg cagatactta
    13501 aagccgacaa tacgggcaaa atcagcgaac gttttttcgc attcggctgg agtatgcaaa
    13561 tcatacccgg cagcgaaagc atggcaggta tcaatgcaga cgccgacgcg ggatttatct
    13621 tccacgccgt cgataatcgc cgcgagatgt tcgaatttaa accctaagtt actgccctga
    13681 ccggcggtgt tttcaatcac cgctgttaca ccttgagttt tatccagcgc aatgttgatg
    13741 gattcggcaa tacgcgcaag gcaatcctct tctgaaatct gcatcagatg gctgccaggg
    13801 tggaagttga gcaaagaaag ccccagttgt tcgcaacgct gcatttcatc tataaaggca
    13861 tcgcgcgatt tttccagagc ttcagtgacc ggatgtccga ggttgatcag ataactgtcg
    13921 tggggaagaa tttgcgccga tgtgtagtga tatttttcac aggcggcttt gaattcatcg
    13981 atggtttgcg tcgtgagcgg tgcggcacgc cactgacgtt ggtttttggt gaacaaggca
    14041 aacgcggttg cgtcgatttc ggcggcgcga attgcggcat ttgccagacc gccagcagca
    14101 ctaacgtgcg ctccaatgta tttcatgcga ggactcctgt taaacccgct ggaggaaaac
    14161 ggtaatgata gcgggttaac aggagcaaga agtagtggtt tatgcgatga cgctttgaat
    14221 cacatagtta atcgcaccac caccaacaat cagccaggca aacagtacca gtgccatcag
    14281 cagaggtttc gccccagctt ttttcagcgc gctgacgtga gtggtcagac ccagcgccgc
    14341 catcgccatt gccagcagga aggtatccag cgttaccagc atgttcacca cgctctgcgg
    14401 taacaggtgg aacgagttaa agatggcaac tacgatgaac aagatggcaa accacggaat
    14461 agtgattttg cttttctcgc cgctgttcgc cccagacagc tgtttaacac gcgccgccag
    14521 caggatgagg aacggagcca gcatcatcac gcgcagcatt ttggaaataa ctgctgcgtt
    14581 ttctgcatcc gggctgatgg catgacctgc cgccaccacc tgcgccactt cgtgcacagt
    14641 agaaccaatg tagataccga aagtttccgg actaaaccat tgagacatca gcggatatat
    14701 cgccgggtag aggaaaatcg cgacggtccc gaagataaca acggttgcaa cagccacggt
    14761 tactttactg gcttccgctt tcactaccgg ctcagtcgcc agtaccgcgg cagcaccaca
    14821 gatactgcta ccggcaccga tcaaccaact ggtgtgctta tccagaccaa acactttctg
    14881 ccccaggaag caagccagca ggaaggtact ggacagcgtc agcacgtcaa tgatgatccc
    14941 actgataccg acatcggcaa tttgcgagaa cgtcagacgg aagccataaa gaatgatacc
    15001 cagacgtaat aaatattgct tggcaaacag cacaccaccg tcacagcttt tccagatgtg
    15061 cggatagatg gtgttgccta aaaccatccc caacaagatt gcgagggtga gggcactaaa
    15121 cccggcaccc gcaaccgcgg gaatggaacc accccacagg gcgaccccgg tgataactgc
    15181 actcagggct aaccccggaa taaaatgcca cagtgtacga tgttgtttct gtaaggtgat
    15241 attcgtcata accctctcct ttaaccgggc ataaggttac gactaactgg tttaaaaata
    15301 aaattgatta tatatttata attaatcttt ataagtggta agcgactatg cacatcaccc
    15361 tccggcagtt ggaagttttt gcagaagtat tgaaaagtgg atcaaccacc caggcgtcgg
    15421 tgatgctggc gttgtcgcaa tcagcagtga gcgcagcctt gactgacctg gaagggcagc
    15481 ttggcgttca actgtttgat cgcgtgggga aaagactggt tgttaatgaa cacgggcggc
    15541 tgctctatcc gcgagcgttg gcactgcttg aacaggcggt tgaaatcgaa caactgtttc
    15601 gcgaagacaa cggcgcgatt cgtatctatg ccagtagtac catcggtaac tacattctgc
    15661 ctgcagttat cgcccgttat cgccatgatt atccgcagtt gccgattgaa cttagcgttg
    15721 ggaacagcca ggacgtgatg caagcggtgc tggatttccg cgttgatatt ggctttattg
    15781 aaggaccgtg ccacagcact gaaatcattt ctgaaccgtg gctggaagac gagcaggtgg
    15841 ttttcgccgc gccgacttcg ccgttggctc gtggtccggt caccttagaa cagctggccg
    15901 ctgcgccgtg gatcctgcgt gaacgcggtt ccggcacgcg ggatattgtc gattatctgt
    15961 tgctgtcaca tttaccgaag tttgagatgg cgatggaatt aggtaactcc gaggcaatca
    16021 aacatgcggt gcgtcatggg ttgggaatta gttgcctgtc gcgacgtgtg attgaagatc
    16081 aattgcaggc aggcacatta agtgaagttg cggtccctct gccgcgcctg atgcgtacgt
    16141 tgtggcgtat acatcatcgg caaaaacacc tttccaacgc gctacggcgc tttctggact
    16201 attgcgatcc cgcaaatgtg ccgcgttaag ttgctgtaca agaacatgct ggtgctgtgt
    16261 cgatttcgtg acgcagcgcc ttcagcatgc attcgccaga aaagagattg gctgctttac
    16321 ttataatccc tgggcgatca tgaaggtgtc ttataaccgt gtatttctgc cggaaggatt
    16381 gccaatcgtc tgctacaatc gcgccatcat ttttaagatg gatagcattt ttgtatgggt
    16441 tccgaaacta aaactacaga agcgccgggc ttacgccgtg aattaaaggc gcgtcacctg
    16501 acgatgattg ccattggcgg ttccatcggt acaggtcttt ttgttgcctc tggcgcaacg
    16561 atttctcagg caggtccggg cggggcattg ctctcgtata tgctgattgg cctgatggtt
    16621 tacttcctga tgaccagtct cggtgaattg gctgcatata tgccggtttc cggttcgttt
    16681 gccacttacg gtcagaacta tgttgaagaa ggctttggct tcgcgctggg ctggaactac
    16741 tggtacaact gggcggtgac tattgccgtt gacctggttg cagcacagct ggtcatgagc
    16801 tggtggttcc cggatacacc gggctggatc tggagtgcgt tgttcctcgg cgttatcttc
    16861 ctgttgaact acatctctgt tcgcggcttt ggtgaagcgg aatactggtt ctcactgatc
    16921 aaagtcacga cagttattgt ctttatcatc gttggcgtgc tgatgattat cggtatcttc
    16981 aaaggcgcgc agcctgcggg ctggagcaac tggacaatcg gcgaagcgcc gtttgctggt
    17041 ggttttgcgg cgatgatcgg cgtagctatg attgtcggct tctctttcca gggaaccgag
    17101 ctgatcggta ttgctgcagg cgagtccgaa gatccggcga aaaacattcc acgcgcggta
    17161 cgtcaggtat tctggcgaat cctgttgttc tatgtgttcg cgatcctgat tatcagcctg
    17221 attattccgt ataccgatcc gagcctgctg cgtaacgatg ttaaagacat cagcgtcagt
    17281 ccgttcaccc tggtgttcca gcacgcgggt ctgctctctg cggcggcggt gatgaacgca
    17341 gttattctga cggcggtgct gtcagcaggt aactccggta tgtatgcgtc tactcgtatg
    17401 ctgtacaccc tggcgtgtga cggtaaagcg ccgcgcattt tcgctaaatt gtcgcgtggt
    17461 ggcgtgccgc gtaatgcgct gtatgcgacg acggtgattg ccggtttgtg cttcctgacc
    17521 tccatgtttg gcaaccagac ggtatacctg tggctgctga acacctccgg gatgacgggt
    17581 tttatcgcct ggctggggat tgccattagc cactatcgct tccgtcgcgg ttacgtattg
    17641 cagggacacg acattaacga tctgccgtac cgttcaggtt tcttcccact ggggccgatc
    17701 ttcgcattca ttctgtgtct gattatcact ttgggccaga actacgaagc gttcctgaaa
    17761 gataccattg actggggcgg cgtagcggca acgtatattg gtatcccgct gttcctgatt
    17821 atttggttcg gctacaagct gattaaagga actcacttcg tacgctacag cgaaatgaag
    17881 ttcccgcaga acgataagaa ataagtttcc tcccttcctt gctaagccct ctcaaccgag
    17941 agggcttttt caattccatt tccctgacaa atcatgcgga tataaaattt aatatttgga
    18001 ttgataattg ttatcgtttg cattatcgtt acgacacaat caaaaaaggc tgacaaatca
    18061 gaggctgttc cggctttctg ggatgatcac ctgcatacaa ataagtccac cgcgatgctg
    18121 ccgtacgcaa ggggacgtaa agaagatgtg agcgataccc attttatttt cgtagttacc
    18181 tcatggagat atggaatgtt taggttgaac cctttcgtac gggtcgggct gtgtttgtcc
    18241 gctatttctt gtgcatggcc tgtgttagcg gtcgatgatg atggcgaaac gatggttgtc
    18301 actgcatctt ccgtggaaca aaatcttaaa gatgcacccg ccagtatcag cgtcattacc
    18361 caggaagacc tgcagcgaaa accggtacag aatctgaagg atgtcctcaa agaagtgcct
    18421 ggcgtacaac tgacgaacga aggggataac cgtaagggcg ttagtattcg tggtctggac
    18481 agcagctaca ccctgattct tgtcgacggt aaacgcgtta actcccgcaa tgccgtcttc
    18541 cgccacaatg atttcgatct gaactggatc ccggtcgatt ccatcgaacg tattgaagtg
    18601 gttcgtggcc cgatgtcgtc gctatacggc tccgatgcgc tcggcggtgt agtgaatatc
    18661 atcaccaaaa aaatcggtca gaaatggtcg ggtaccgtta ccgtcgatac caccattcag
    18721 gaacatcgcg atcgcggtga tacctataac ggtcagttct ttaccagtgg accattaatt
    18781 gatggtgtgc tgggaatgaa agcttacggc agcctggcaa aacgtgaaaa ggatgacccg
    18841 caaaactcaa cgaccaccga taccggagaa acgccgcgta ttgaaggatt ctccagccgc
    18901 gacggcaatg tcgaatttgc ctggacaccg aatcaaaatc acgattttac tgccggatac
    18961 ggtttcgacc gtcaggatcg tgattccgat tcgctggaca aaaaccgcct ggaacgccag
    19021 aactactgcg tcagccataa tgggcgttgg gattacggca ccagcgaact gaaatcctac
    19081 ggtgagaaag tcgagaacaa aaaccctggc aacagcagcc cgataacttc cgaaagcaat
    19141 acggtcgacg gcaaatacac gttgccgctg acggcgatta atcagtttct cacggttggc
    19201 ggtgaatggc gtcacgacaa acttagcgat gcggtgaacc tgaccggggg aaccagctcc
    19261 aaaacgtctg ccagccagta cgcgctgttt gtggaagatg aatggcggat cttcgagccg
    19321 ctggcgctga cgaccggcgt gcgtatggac gatcacgaaa cctacggtga acactggagt
    19381 ccgcgtgcct acatggttta taacgccacc gacaccgtaa cggtgaaagg gggctgggcg
    19441 acggcattta aagcaccttc tctgttgcaa cttagccctg actggacgag caattcctgc
    19501 cgtggcgcat gtaagattgt gggtagcccg gatctgaaac cagaaaccag cgaaagttgg
    19561 gagctggggc tttactacat gggtgaagaa ggctggctgg aagggggtga atccagcgtt
    19621 atcgttttcc gtaacgatgt gaaagatcgt atcagcatca gccgtacgtc tgacgtcaac
    19681 gctgcaccgg gctaccaaaa ctttgttggt tttgagacgg gcgctaacgg acggcgcata
    19741 ccggtattta gctactacag cgttaacaaa gctcgtattc agggcgtgga aaccgaactg
    19801 aaaattccgt tcaacgatga atggaaactg tcgatcaact acacctacaa cgatggtcgt
    19861 gatgtcagca acggcgaaaa caaaccgcta tccgatctgc cgttccatac tgctaacggt
    19921 acgctggact ggaaaccgct ggcgctggaa gactggtcat tctatgtttc tggtcactat
    19981 accgggcaga aacgcgccga cagcgcgacg gctaaaacac cgggcggtta taccatctgg
    20041 aataccggcg cggcctggca ggtgactaaa gacgtcaaac tgcgcgcagg cgtgctgaac
    20101 cttggcgaca aggatctcag tcgtgacgac tacagctata acgaagacgg acgtcgttac
    20161 tttatggcag tggattatcg cttctgatga gaagatgccc ggcgaaccgg gcggactttc
    20221 acttcagtaa atactgcgca tggaagcgca ggtgatcctc tataaaagag gcgatgaagt
    20281 agtaactgtg atcatatccc ggctgaatac gcagcgtcat cggccacgct ttctggcgcg
    20341 cggcttctgc caataccgca ggttgcaact ggtcggcaag aaactgatca ttatcgccct
    20401 gatcgataag cgtcgggatc gcatcctgcg cgttactggc atacatcagc gcgcaactat
    20461 cccattccag catgcatttt tgtcctcacc taaataggtg ctaaacgctt tgattcccca
    20521 cgggacgctg cacggattca caatgggcgc aaaggccgaa acgctggtgt atttacccgg
    20581 atttttcagc gccataatca gcgcaccgtg accacccatt gagtgaccgc taatggcgca
    20641 gcggtcgctg acattaaatt gcgactgaac cagcgccggt aattcatcgc gcagataatc
    20701 atacatccgg taatgcgtcg cccacggcgg ttgcgtggca ttaagataaa agcctgcgcc
    20761 ctggcctaaa tcgtagccat cgtcgttggc aaccttttcg ccgcgcgggc tggtgtctgg
    20821 catcaccagt acaatcccca gttccgccgc tacccgctgg gcacccgcct tggtggtgaa
    20881 gttctcgtca ttgcaggtta atccggaaag ccagtacagc actggtggcg gggtgtgatc
    20941 acgagggggt gggagaaaga tactgaacgt catcgggcag tttaaggtac tggagtcgtg
    21001 tcgccagcgt tgctgccagc cttcaaaaca gcggtgctct tcgagcattt ccatgcgtgg
    21061 ctccttgttg tgttgtttgc aatttcttca tcataccgtt tattcaatgt gctgtgagta
    21121 actttcactt ccgtatttgc ataacgatgt tttaacatct gctgatgaaa ggcagcggca
    21181 attacaataa ttatcgctgt gaatactgga ttatgtgcgc cgcctcacgc acaataatca
    21241 agctgtaaat cagcttaata actttgcccc cacgcagggc ggaggcgtca cacctgcagg
    21301 agaaatcata aatgccatca ctcagtaaag aagcggccct ggttcatgaa gcgttagttg
    21361 cgagaggact ggaaacaccg ctgcgcccgc ccgtgcatga aatggataac gaaacgcgca
    21421 aaagccttat tgctggtcat atgaccgaaa tcatgcagct gctgaatctc gacctggctg
    21481 atgacagttt gatggaaacg ccgcatcgca tcgctaaaat gtatgtcgat gaaattttct
    21541 ccggtctgga ttacgccaat ttcccgaaaa tcaccctcat tgaaaacaaa atgaaggtcg
    21601 atgaaatggt caccgtgcgc gatatcactc tgaccagtac ctgtgaacac cattttgtta
    21661 ccatcgatgg caaagcgacg gtggcttata tcccgaaaga ttcggtgatc ggtctgtcaa
    21721 aaattaaccg tatcgtgcag ttctttgccc agcgtccgca ggtgcaggaa cgtctgacgc
    21781 agcaaattct tattgcgcta caaacgctgc tgggcaccaa taacgtggct gtctcgatcg
    21841 acgcggtgca ttactgcgtg aaggcgcgtg gcatccgcga tgcaaccagt gccacgacaa
    21901 cgacctctct tggtggattg ttcaaatcca gtcagaatac gcgccacgag tttctgcgcg
    21961 ctgtgcgcca tcacaactga ttaaaaggca ggaaccatgg agcgcaacgt cacgctcgat
    22021 tttgttcgcg gcgtcgccat tctggggatc ctgctattaa acatcagcgc ctttgggcta
    22081 ccaaaggcgg cttatctcaa tcccgcctgg tacgatgcta ttacgccgca ggatgcatgg
    22141 acctgggcat ttctcgatct catcggccag gtgaaattcc tcacgctttt tgcgctgctg
    22201 tttggtgctg gcctgcaaat gttgctgccc cgtggcagac tctggatcca gtcgcggtta
    22261 acgctgttag tcttgttggg ctttattcac ggtttattgt tctgggacgg cgatattctg
    22321 ctggcttacg ggctggtggg gcttggcgtt ttgctgttat tggggctgat ttccgacagc
    22381 cagaccagcc gcgcctggac gccggatgca tcggctattt tgtatgaaaa atactggaag
    22441 cttcacggcg gcgttgaagc gatcagtaat cgtgccgatg gtgttggcaa cagtttactg
    22501 gcactgggcg cacagtatgg ctggcaactg gcagggatga tgctcattgg tgcggcgctg
    22561 atgcgcagcg gctggctgaa agggcagttc agcttacgtc actatcgtcg tactggtttt
    22621 gtgctggtgg cgattggggt gaccattaac cttcctgcca tcgccctgca atggcagctg
    22681 gactgggcgt atcgctggtg tgccttctta cttcaaatgc cgcgggaact gagtgcgccg
    22741 tttcaggcga ttggctatgc gtcgctgttt tatggcttct ggccgcaatt gagccgcttt
    22801 aagctggtgc ttgcgatcgc ctgcgtcgga cggatggcgc tgaccaacta tctattgcaa
    22861 acgctgattt gtaccacgct tttttaccac ctcggtttgt ttatgcattt tgaccgcctg
    22921 gagctgctgg cgtttgttat tccggtatgg ctggcgaata tcctcttctc tgttatctgg
    22981 ctgcgttact tccgccaggg gccggtggaa tggctctggc gtcagttaac tttgcgtgct
    23041 gccggaccgg caatatctaa aacatcaaga taacgatctg gattacattc gttaacaaaa
    23101 cggctgtaac cgtttccatt gctgtgactc gattcacgaa gtcctgtatt cagtgctgac
    23161 aaaatagccg ccagcaagca gtcatttact gcaatctcat aacaggtagt gaatatgatc
    23221 accattcgtg atgtagcgcg tcaggctggc gtctctgtgg caacggtttc ccgggtgctc
    23281 aataacagca cgctggtcag tgccgacacg cgtgaagcag taatgaaagc cgtgagtgag
    23341 ctggattatc ggccaaacgc caatgcccag gcgctggcaa ctcaggttag cgacaccatt
    23401 ggcgtggtgg tgatggacgt ttctgatgcg tttttcggcg cgctggtaaa agcggtggat
    23461 ctggtcgccc agcagcatca gaaatacgtg ctaatcggca atagctatca tgaagcggaa
    23521 aaagagcgtc acgccattga ggtgttaatt cgccagcgtt gtaatgcgtt gattgttcac
    23581 tcaaaagcat tgagtgacga tgaactggcg caatttatgg ataacattcc cggtatggtg
    23641 ttaatcaacc gcgttgtgcc ggggtacgcc catcgttgcg tttgcctgga taatctcagc
    23701 ggtgcccgaa tggcgacgcg catgttgctg aataacggtc atcaacgtat tggttatctt
    23761 tcttccagcc acggcattga agatgacgcc atgcgtaaag caggctggat gagtgcgttg
    23821 aaagagcagg atattattcc gccggaaagc tggattggca ctggtacgcc ggacatgccg
    23881 ggcggtgagg cggcgatggt tgaactgctg gggcgcaatc tacaacttac cgctgtattt
    23941 gcttataacg acaatatggc cgctggcgca ctgacagcat taaaagataa tggcattgcg
    24001 attccgttac atctctcaat catcggtttc gatgatattc ccatcgcccg ttacaccgac
    24061 ccgcaattaa cgaccgtgcg ttatcccatt gcttcaatgg ctaaattagc caccgaactg
    24121 gccttgcagg gggcagcagg caatattgat cctcgtgcca gccactgttt tatgccgacg
    24181 ttagtgcgtc gccattctgt cgcaacgcgc cagaatgcgg cggcgatcac taactcaaca
    24241 aatcaggcga tgtaaccgct ttcaatctgt gagtgatttc acagtatctt aacaatgtga
    24301 tagctatgat tgcaccgttt taatgttgta acccgtatgt aacagtgaat aatcactttc
    24361 accgaggtaa tagcgtcata acaacaatta aagccgtttt ctggagcgtt accgggcatg
    24421 gaagaacgaa ttttaaaaag tgagcttcgg cgttcagtaa cacttcatta actctactgc
    24481 cccgccgagc atttatctca agcactaccc tgcataagaa aaaccggaga taccatgaat
    24541 aagaaggtgt taaccctgtc tgctgtgatg gccagcatgt tattcggtgc cgctgcacac
    24601 gctgctgata ctcgcattgg tgtaacaatc tataagtacg acgataactt tatgtctgta
    24661 gtgcgcaagg ctattgagca agatgcgaaa gccgcgccag atgttcagct gctgatgaat
    24721 gattctcaga atgaccagtc caagcagaac gatcagatcg acgtattgct ggcgaaaggg
    24781 gtgaaggcac tggcaatcaa cctggttgac ccggcagctg cgggtacggt gattgagaaa
    24841 gcgcgtgggc aaaacgtgcc ggtggttttc ttcaacaaag aaccgtctcg taaggcgctg
    24901 gatagctacg acaaagccta ctacgttggc actgactcca aagagtccgg cattattcag
    24961 ggcgatttga ttgctaaaca ctgggcggcg aatcagggtt gggatctgaa caaagacggt
    25021 cagattcagt tcgtactgct gaaaggtgaa ccgggccatc cggatgcaga agcacgtacc
    25081 acttacgtga ttaaagaatt gaacgataaa ggcatcaaaa ctgaacagtt acagttagat
    25141 accgcaatgt gggacaccgc tcaggcgaaa gataagatgg acgcctggct gtctggcccg
    25201 aacgccaaca aaatcgaagt ggttatcgcc aacaacgatg cgatggcaat gggcgcggta
    25261 gaagcactga aagcacacaa caagtccagc attccggtgt ttggcgtcga tgcgctgcca
    25321 gaagcgctgg cgctggtgaa atccggtgca ctggcgggca cagtactgaa cgatgctaac
    25381 aaccaggcga aagcgacctt tgatctggcg aaaaacctgg ccgatggcaa aggtgcggct
    25441 gatggcacca actggaaaat cgacaacaaa gtggtccgcg taccttatgt tggcgtagat
    25501 aaagacaact tggctgaatt cagcaagaaa taagactgat tgggcgcagt cgtttactgc
    25561 gcccttattg tatggccaac aaggtaaaat tatatggtca gctcaacgac tccgtcttcc
    25621 ggggaatact tgttggaaat gagcggtatc aacaagtcct ttcctggtgt taaggcactt
    25681 gataacgtta atttaaaagt ccggccacat tctatccatg cattaatggg ggaaaacggt
    25741 gcaggaaaat cgacattatt aaaatgcctg tttggtattt atcaaaaaga ctccggcacc
    25801 attttattcc agggtaaaga gatcgatttc cattccgcca aagaggcact ggaaaatggt
    25861 atttcgatgg tacaccagga gttaaacctg gtattacaac gttcggtgat ggataacatg
    25921 tggctggggc gatatcccac caaaggcatg tttgtcgatc aggacaaaat gtaccgcgaa
    25981 accaaagcga tttttgatga actggatatt gatatcgatc cgcgtgcgcg cgtcggcaca
    26041 ttatccgttt cgcaaatgca gatgatcgaa atcgccaaag cgttttccta taacgcgaaa
    26101 attgtgatta tggatgaacc gacttcttcg ttaaccgaaa aagaggtcaa tcatctgttc
    26161 actattattc gtaaattaaa agagcgcggc tgcggtattg tttatatctc gcataaaatg
    26221 gaagaaatct tccagttatg tgatgaagtt accgtattgc gcgacggtca gtggatcgcc
    26281 accgaaccgc tggcaggact gacgatggac aagatcatcg ccatgatggt tgggcgttct
    26341 cttaatcagc gttttcctga caaagaaaac aagccgggcg aagtcatcct cgaggtgcgt
    26401 aacctaacgt cactgcgcca gccgtcgatt cgcgatgtct cgtttgatct gcataaaggg
    26461 gagatcctcg gtattgccgg gctggtgggg gcgaaacgta ccgatattgt tgagacgtta
    26521 tttggtattc gcgagaaatc ggctggcacc attacgttgc acggcaaaca gatcaataac
    26581 cataatgcca acgaagccat aaaccacgga tttgcactgg taactgagga gcgccgctca
    26641 acgggaattt atgcctatct ggatattggt tttaactcgt taatttccaa tattcgcaac
    26701 tacaaaaata aagttggttt actggataac tcgcggatga aaagcgatac ccagtgggtg
    26761 attgattcga tgcgggtaaa aacgccgggt catcggacgc aaattggttc gctctccggt
    26821 ggtaatcagc aaaaggtgat tattggtcgc tggctactaa cgcaaccaga aatattaatg
    26881 ctcgatgaac cgacgcgcgg tattgatgtc ggggcgaagt ttgaaattta tcagttaatt
    26941 gccgaactgg cgaagaaagg caaggggatt attattatct cctctgaaat gcctgagttg
    27001 ttagggataa cagaccgtat tctggtcatg agcaatggtc tcgtttccgg aattgtcgat
    27061 acaaaaacaa caacgcaaaa cgaaattctg cgtcttgcgt ctttgcacct ttaagattag
    27121 gggcttccca tgagtgcgtt aaataagaaa agttttctta cttacctgaa agagggcggt
    27181 atttacgtcg ttcttttagt tttgctggcg attattattt tccaggaccc aacattttta
    27241 agtctgttga acttaagtaa tattctcacc cagtcatcgg tgcgtattat tatcgcgctc
    27301 ggtgtggcag ggttaattgt cacccagggg accgatcttt ctgctggtcg tcaggtaggg
    27361 ctggcggcag tggtggctgc gacattattg cagtccatgg ataacgccaa caaagtgttc
    27421 ccggaaatgg cgacgatgcc gattgcgctg gttattctga ttgtctgtgc cattggtgcg
    27481 gtgatcggtt tgatcaacgg tctgattatc gcttatctca acgtgacgcc gttcattacc
    27541 acgctcggca cgatgatcat cgtctatggc atcaactcgc tctattacga ctttgtcggg
    27601 gcgtcgccaa tttctggttt tgacagtggc ttctctacct ttgctcaggg ctttgtcgcg
    27661 ctggggagtt tccgtctctc ttacatcacc ttctacgcgt tgattgcggt ggcgttcgtc
    27721 tgggtgttgt ggaacaaaac ccgcttcggt aagaacattt ttgccattgg cggtaacccg
    27781 gaagcggcaa aaatatctgg tgtcaacgtc ggcctgaacc tgctgatgat ctacgcgttg
    27841 tctggcgtgt tctatgcctt tggcgggatg ttagaagccg gacgtatcgg ctctgccacc
    27901 aacaacctcg gctttatgta tgagctggat gctatcgcgg cgtgcgtggt aggcggtgta
    27961 tcgttcagcg gcggtgtggg gacggtgatt ggcgtggtga ccggggtaat tatttttacc
    28021 gtcatcaact atggcctgac gtatatcggc gtaaacccat actggcagta catcatcaaa
    28081 ggggcgatta ttatcttcgc cgtagcgctg gattcactga aatacgcgcg taagaagtga
    28141 tgtgattgcg gatattgccg gatgcgtctc tggttttaca acgttaccgg gtgttctttc
    28201 tcgcctttct taaacttcac ttccccgagc tcaatacaac ccaccgggca gacgtgacca
    28261 caaagcagac aacccacaca tttctcggta ttacaatgcg gtgtgcgggt tttctcgctc
    28321 cattccatcg cctggtgacc gccgtcgtaa caggaaatat aacagcgtcc acagccaaca
    28381 catttgtcaa gattgatacg gggatagaca atataactgc ggtctaaatc ttccgccggg
    28441 acaatattgt tattcgccag acctaccatc tcctgcaacg aatcaaatcc ctgatcggcg
    28501 agataatgcg ataacccgct cgccatatct tctacgatgc gatagccgta ctgcataatg
    28561 ccggtggtga cctgtaacgt tgctgcgccg agcaataaaa actccgccgc atcttcccag
    28621 gtttcaatgc cgccgatacc gctgattggg aaatcgcgca gttctggatg ggtgcgcatt
    28681 tgctgaatga agcgcagggc gatcggtttt accgctttac cggaatatcc ggaaatactc
    28741 gatttaccgt taacgatcgg cataccgatt ttctgattaa gatcgatatt ggtgatggat
    28801 ttaacggtgt taattgccgc aatgccatct gcgccgccgc gcttcgccgc cagcgccact
    28861 tcgcacatat caccgatatt cggcgtcatc ttcgccagca ttggcagcgt ggaaccccgt
    28921 ttcactgccc gacaatattt ttctaccagc tccgggcttt gcccgacatc gctacccatc
    28981 gcatgagaag tcatttgcgg acaggagaag ttacactcga tcatatccgc gccagctgct
    29041 cccattgctg ctcattttcc cccatgatcg aagcgatcaa tactttgtcc gggtaatctt
    29101 ccttcagccg acgcagggcg gtcagattct cttccaacgg atgttcagca atctgctcca
    29161 tatttttgaa gccgataaaa ccggtatctt ctttcaccag atgatcaaaa cgcggcgaga
    29221 cttcgttggc gataaaaaag ccgatcgttt taaacaccac accgccccaa cctgtgtcgt
    29281 aggctttggc acacatctca tagcagttgc ctaccggcga agaagagagg cagaacgggt
    29341 tgggaaagtt cacgccgcaa aaagtaatcg aaagatcttt cgttaacatg agcaagctcc
    29401 ctctaaatag tgatgaatcg cctcggcggc ttctttcccg gttttcacgg catagaccac
    29461 ggttttgtca ccctcaacaa tatcgccagc agcaaagact tgcggatcgc gggtctgata
    29521 attttgtgtt ttgatggtgt tacgctgctg ctctaactcc gcaaaggcat ccagtctggc
    29581 atgctgaccg acggcgagaa taattttatc tgccgccatc gtcagttcgc ctgataaccg
    29641 tacatgctta aacgtgactt tattgccttc cacggctact ggcgtgaatc catcgatgat
    29701 cgaaacgccc agctcccggg cgctggcaaa ctctttttcg cttgccggaa actcatctaa
    29761 ctcttcacgt gctacgcaag ttaccgcctg acagccgaga actttcagcg tgctggctac
    29821 gtccatcgcg acatcaccgc cgccgataat taatgcgctt tgtggaatgc tgatatcgcc
    29881 ttgcgcctgt cgtacacgtt gcaggaagtc gacggcaatc tcaacgtcgc tatgctcaaa
    29941 cagcggtagc ccggaaccgc ttgataaccc cacggtgacc agtaccgcgc ggttttccgc
    30001 tttcagttgc tccagggtga gtgttttacc gatttcgtta ttgcacttaa tggtcacgcc
    30061 cattttttca atacgggcga tctctgcatc cagcactgat tgcggtaacc ggaattgcgg
    30121 aataccgtta cgcaaccagc caccggggtg cgcttctttc tcataaatcg tgacgtcata
    30181 accctggttt gtcagtgtca cactggcctg caatccggca ggacctgcgc caataatcgc
    30241 gactttgccg agcgttttag taccgggctg ataaatttcc atcccggttt gttgttcaaa
    30301 atcagtaaca aaacgctgta agcggccgat atcaatggga gcatcaacac cggcacgggt
    30361 acaaccgctt tggcataatt tctccgtcgg gcacactctg gcacaaacgg caccgagggc
    30421 attattttcg cgaattgtct cggcagcgcc tttaaaatta cgaaagtaga ttgagcgaat
    30481 aaatttcccc ggatcggtct gtgccgggca agcctgacta cagggagcgt cgtgacataa
    30541 taaacagcga gaggcttctt taatcgccag taacggcgta aaagccggag tgagttcatc
    30601 cagataattt tgttgcggca tgtgatcttc cttaatgcta tgagtaatta tggcgaacgc
    30661 taaataaata ttgcgtgctg atattaaggc tatgcgcgtg gggaaattta agctgtgaaa
    30721 ggattcacaa atagctgcgg ttcagaatgt tatatccgca gcttattgag tgaagtaata
    30781 attattctca ataattaaca gaaaagagaa aatattaacg atagaaaata agataaacaa
    30841 tcatgccgat agataaaatt ccgaagccaa tatatgccac catataagcg aagaagagtc
    30901 cgggattttt tgctcgccag acatacaccg gttccggtgc atttttaacc cgtttatcaa
    30961 tcgcgcctgc gcgccagcct ttccatgctt cgcggaaaca aaatatcggg ataaggaaaa
    31021 aaacggcaac gacaaaaaac tgctgtacat ccataactac tttccttgtt tcttttgtaa
    31081 ttcaagtaac tgttcgggtg taacggcggg atacccctgc gcatcttccg gcacctggtg
    31141 catagccgga atagggacca gcggccctaa aaaacgcggt tcacgtttaa aaatataaag
    31201 gtcagccagc gcaccgaaac gggcggcaaa ttcgcgaata cgtactgaca gcatatcttt
    31261 cggtgacggt acggcataac actgagcctg aatccccata tgcagcgcaa taaataatgc
    31321 tcgctcacag tggaaacgtt gggtgataat aatgaaatca ttagtatcga aaactttgcg
    31381 tgtacgcacg atggaatcca gcgtacgaaa gcctgcgtaa tcgagaacaa tatctgatgg
    31441 gtcgacacca gcagcgatta aatctttgcg catggtcatc ggctcgttat aactttgcaa
    31501 tgcgttatcg ccgctcagta atagataatt taccttaccg ctgttatagg cattaatcgc
    31561 tccttgaatg cggtagcgat aatactgatt aataacgccg gtacgataat attttgctgt
    31621 gccgagcacc acgccgacct ggcggtaggg gagatcctgc aattcgtcgt agatataagg
    31681 cgcggttttc cagctcatcc agcgatcgag gccgagcaca gtcaacagca gcaagccgat
    31741 caggactaac aggctgagga acacgcgctt taacatgcag gagactcgag ctaaggaaaa
    31801 tttccatcag gctactttac ccgctggttt tgcgcaagaa acggcaaact gtttgccggg
    31861 aatttcgaca ccaggagagg gctggcaagc cggacaaggc gttcacgccg catccggcac
    31921 caggcttaag cgagaagcac gcggtcgata ctgtgacagc cgagagcttt cagcgttgcg
    31981 gaggtggcat cccactgaat caacggcgca tcggcttttt ctgccagaac cgcgcgctgg
    32041 atatccgggt aatcataacc cttgagattc aacagaatta acgccccctg caacggcggc
    32101 agagtcgggt tgaatgcggc gttttcagcg tagctgccgc tgaaaatacg accgtcttta
    32161 cattccagcg cgacaccgct tggtgactta ctgtaaggca tgtgcgaacg gtttgccgct
    32221 gcaatcgccg cctgagaaag cgcatcacca gtcagcgcat agccgtgatc ctgctcgtcc
    32281 atcagcagcg ttttaatctc cagatctttc ggcccaaagg catccggcag atagtcacgc
    32341 agcgcgtgtg cctcgcggcc cggcaaatga atacgcagat ccagaccgct gttcagttca
    32401 ttcataaact gacggcagtg accacaaggc gtatagttaa cggtgatggc tgcaagcgct
    32461 ttttcaccac tcaaccaagc gtggctgatt gcgctttgtt cggcatgaac ggtttgctgc
    32521 attgttgcac caataaattc catattggca ccaaaatacc aggttccgct cacaccgcgc
    32581 gcaattgcgc caacattaaa attcgacaat ggcgtacgcg cacaggccgc cgccagcgga
    32641 agtagtgcga atgccagcgc gtcttcgtcc agtcccgttg cgctcttcag cgatgagact
    32701 tgctccccgg tcaacaaagc ggggaagtac ttgtctgcca gaataggttc cagtgcagat
    32761 tgcaagttat ccgcaagttg ggcaaaagcg gtttgaaaac gtggatgcat ggcgttgcct
    32821 cataatcatg taatgggata acagtttacg aacccgttat ggctttatat gtgatctgaa
    32881 tctcattaat tatgcaacac gtgaaactta catcaaaaat gcgcgacgta tcgcaaattt
    32941 tagcccatta ctgccagaat aatcgggaaa aggaacggtg cgatcagcga agtaattatc
    33001 ccgcataaca ccagcgctag cgaactaaat gcaccttcct gataatccag ctcggcgcag
    33061 cgcgccgtac cgagggcgtg cgaggcagtc cccattgcca gaccgcgcgc agctttggta
    33121 cgaatacgca tcgcattaag caatgtatgg ccaaataccg cgccgaggat gccgacgaaa
    33181 atcacgcaaa ctgcgctgat tgccggaata ccgccaatac tgccgccaac cgccattgca
    33241 ataggcgtgg tgactgattt cggcaggatt gacgcggcga tttccggtga agcgcccatc
    33301 aacaatgcca cggaagtccc cgtcaccatc gcaaccacgc tgccgataaa acagatggtg
    33361 atgatcgatt tccagcgcgc gcggatctgg tgtagctgct catataaagg ataggccagc
    33421 gcgacgaccg ccggttgcag cagatcgttt agcacttcgc tacctttaaa gtaactgtcg
    33481 taagagatgc cggtcagcat taaaaaagga atgatcacca ccattgctac cagcaacggg
    33541 ttaagcaggg gaaacttata ccgtgccgcc agtttgcggg cggcaaaaaa gacaatcaac
    33601 gtcagcggta atgaccacca gatattcgcc atcattcttc tgatcctttc tgacctacga
    33661 ctttacgttc accgtgtacc agttgtgaac tccagctcac caccagaaaa accaccagcg
    33721 tactgacagc acaggaaacc actaccgggc caaactgcgc gcggagcaaa tcaaaatatt
    33781 gcatgacgcc tacgccaatc ggcacaaaca atagcgccat atagcgaatc agtacgtagc
    33841 accccggatt gacccatttc gccggaagaa tttgcaaggc cagcaggaca aacaggatct
    33901 gcatcccgat gatgctgccc ggaatggtta ccggtagcag ggaagcaatg aaaatgcctg
    33961 catacaggca ggcataaatc aggatgaaag cgcgtaaata ttgccagata atgttcagtg
    34021 tcttactcat aatcaacacc ccagatgaga tggattcatc atacaattaa ttcgtaaaac
    34081 gtgctaccga tcacattaag aaatcagcgc ttattttttc gctacccggt attacgcaat
    34141 gagcagcgaa ctcaggcata catcaagcat agagagggat tgcagtttgc agcaatccct
    34201 ctttgcagac ctttaatccg ctgaccatag gcgctaacat agcgcctcat ttttttgcgg
    34261 gtgatgatat gcgtgtgtta ctggcaccga tggagggagt gcttgactct caggtgcgtg
    34321 aattgctgac cgaagttaac gactacgatc tgtgcatcac cgagtttgtc cgcgtggtgg
    34381 atcaactgct gccggtaaaa gtctttcatc gcatttgccc tgagctacaa aacgccagcc
    34441 ggacaccatc tggtacgctg gtgcgcgtgc agttgttagg tcagttccca caatggctgg
    34501 cagagaacgc cgcccgtgcg gtggagttag gtttctgggg cgtggatctc aattgcggct
    34561 gcccgtcgaa aacggttaac ggtagcggcg gcggggcgac gttactcaaa gatcctgaac
    34621 tcatctacca gggggcaaaa gcgatgcgtg aagctgtacc ggcgcatttg cctgtcagcg
    34681 tgaaagtgcg tctgggctgg gacagcggtg agaagaaatt tgaaatcgcc gatgcggttc
    34741 aacaggctgg cgctacggag ctggtggtgc atgggcggac gaaagagcag ggttaccgcg
    34801 cggagcatat tgactggcag gcgattggcg agattcgcca gcggctgaat attccggtga
    34861 ttgccaacgg tgaaatctgg gactggcaga gcgcgcaaca atgcatggcg atcagcgact
    34921 gcgacgcagt gatgattggt cgcggggcgt tcaatattcc caacctgagc cgggtggtaa
    34981 aatataacga accgcgaatg ccgtggccgg aggtggttgc tttgctgcaa aaatataccc
    35041 gtctggaaaa gcagggcgat accgggttat atcacgttgc gcggattaaa cagtggttga
    35101 gttatttgcg taaagaatac gatgaagcaa cggaattatt tcagcatgtt cgggtgttga
    35161 ataactcccc tgatattgca agggctattc aggcaattga tatcgagaaa ctctaaaaac
    35221 catagtgatg tttttgacaa tgtgttagct ctttatcaat aggccgcacg gttgtttggt
    35281 ggcgtcaaaa tcgccgaggc gttccctgaa ggccggggca gcccacatgg atgtgggctg
    35341 agggcgcgtt ttacagggat gttacctcgc gcccgacccg gtagccgtaa gggataagcc
    35401 gagggcaccg cgcagcggcg attttgttcg ccagagcccg ggggggcagg gggcggcggc
    35461 gattggtcgc cccctgcgcg ctccttgcgc cagtgattat atgaagcaat gcacatgaca
    35521 ggagcgcaac aggatgagag actgaaatat tctcgttatt ttcggatctg atgacattaa
    35581 ttaattttaa aatatcatct taaatacgcc agtcaccacc agtagcccaa tcaggaaaat
    35641 aatcaataca gcccagagta taattttcat tggcgttcct ttatttgtga gtgctgaata
    35701 agtataggaa aaaacgaagt cctttgccgt tatccataaa cgattaagat aaatcggaag
    35761 atatcattta cttagtctat atttttctgg gtatgtcgtt ttattgctac tgatgtattg
    35821 ccacgcgaaa ctaaaaaata tatcgcaaca cacggtaatc tccgcgcacc gtttcttacc
    35881 tgattattcc cccatgaatc gtgattcctt ttatccagcc atcgcctgtt ttccgctgtt
    35941 actgatgctg gccgggtgtg cgcctatgca tgaaacccgc caggcgttaa gccagcaaac
    36001 gcccgctgca caagttgaca ccgcattacc cacggcgctg aaaaatggtt ggccagacag
    36061 ccaatggtgg ctggagtatc acgataatca gctcacttca ttaattaaca atgcactaca
    36121 gaacgcaccg gatatgcagg tggctgaaca gcgcattcag ctagctgaag cccaggcgaa
    36181 ggcagttgcc actcaggatg gtccgcagat cgacttttcg gcggatatgg agcggcaaaa
    36241 aatgtcggca gaagggctaa tggggccgtt tgctctgaac gatccggccg caggtacgac
    36301 cggtccgtgg tacaccaacg gaacttttgg cttaacggcg ggctggcatc tcgatatctg
    36361 gggaaagaat cgggcggagg ttactgcccg cctgggtacg gttaaagcac gggcggcgga
    36421 acgcgagcaa acccgccaat tgctggctgg cagcgtagcc cgcctgtact gggagtggca
    36481 aacccaggcg gcgttaaaca cggtcttgca gcaaatagaa aaagagcaga acaccattat
    36541 cgcgaccgat cgccagctat atcagaacgg gattacttct tcagttgacg gtgtggaaac
    36601 cgatattaat gccagcaaaa cccggcagca gctcaacgat gtcgcgggga aaatgaaaat
    36661 tattgaggca cggttaaatg cactcacaaa tcaccagact aagtcattga agcttaaacc
    36721 ggtcgcgttg ccgaaagtgg caagccagct tcctgatgaa ctggggtact ccttactggc
    36781 ccggcgggca gatttgcagg cggcgcactg gtacgttgag tcatcgttaa gcactattga
    36841 tgcggcaaaa gcggcatttt atcctgacat caacctgatg gccttcctgc aacaggatgc
    36901 gttgcactta agcgatctgt tccgtcattc cgcgcagcaa atgggcgtta cggcaggcct
    36961 gacgctacct attttcgata gtggtcgtct taacgccaat ctcgatatcg caaaagccga
    37021 aagcaacttg tctatcgcca gctacaacaa agcggtggtt gaagcagtga atgacgtggc
    37081 gcgggcagcc agtcaggttc agacactggc ggagaaaaac cagcatcagg cgcaaattga
    37141 gcgcgatgcc ttgcgtgtgg taggtcttgc gcaggcccgc tttaacgcgg gcatcattgc
    37201 tggttcccgc gtcagcgaag ccagaatccc ggcgctgcgt gagcgggcca atggcctgtt
    37261 attgcaaggg cagtggctgg atgcctccat tcgactcacc ggtgcgctgg gcgggggtta
    37321 caaacgctga tgatatgcgc cttctatact taacgtttat tcagcgttaa gtggagaact
    37381 cgatggcata ggtcgcgata attaccgctt ccgattcggg gatcggcaaa gagtgcgcgt
    37441 tattactggc gcagcagggg tttgatattg gcattacctg gcactcagat gaagaagggg
    37501 caaaagatac cgcgcgtgag gtggttaccc acggcgtacg tgcggagatc gtgcaactgg
    37561 atctcggcaa tctgccagaa ggtgcgcagg cgctggagac actcatccag cgatttgggc
    37621 gtattgatgt gctggtgaat aatgcgggtg caatgaccaa agcgccgttt cttgatatgg
    37681 cttttgatga gtggcgcaag atttttaccg ttgatgttga tggtgcattt ttatgctcgc
    37741 aaattgcggc tcgtcagatg gtgaaataag ggcagggcgg tcgcatcatc aacattacgt
    37801 cggtacatga acatacgccg ctgccggatg ccagcgccta cacagccgct aaacatgcgc
    37861 tcggcgggtt aaccaaaacg atggcgctgg agttggtcag gcataagatt ttggtgaacg
    37921 cagtcgcgcc tggggcgata gccacgccaa tgaacagcat ggatgatagc gacgtgaagc
    37981 ccgacgcgga gccttcgatt cctctacggc gttttggcgc aacgcatgag attgccagcc
    38041 tggtggtgtg gctttgttcg gagggcgcaa attacacgac agggcagtcg ttgatagtgg
    38101 atggcggctt tatgttggcg aatccgcagt tcaacccaga atagcgcagt gaggtgcgtg
    38161 gatatttcaa tgggttaggc cagataagac accagcgtcg catcaggcat ggaacacccg
    38221 gtgccggatg tggagtgaat gcttatctgt ggcaactgct ttacgcctga ttatccggct
    38281 ttttcttccc gcgccgtttc agccaccagc gcacgcccac caccagaacc acgaccagaa
    38341 tcaaccagac ccagtgcttc aaatgctggt cgagattgtg caaccacggc gcaatcacct
    38401 gaccaccagc gtaaccaata gtggtaaaaa tcaacgccca ggcaaatgcg ccgagaatat
    38461 tcagcggcag aaagattttc ggcggcagct ggctggcacc aatcagcgtc gggccaatca
    38521 cccgaaagcc atacataaag cgcgtaccaa tgacaaacag atacggatgg cgttggataa
    38581 gtttctgtgc ccgctcaatt ttatcctgat gtttcgagaa acggcgtaac aacttgccgc
    38641 caaaccgccg cccgcacaga tagagcacct ggtcgccaat catgccacca agcgccacag
    38701 aaagtaccac cagtgggaac tttaatagcc cctgatgtgc cgcaacgcct cccagcaaag
    38761 tcacggtttc accttccgcc aggctaccga taaccagcgc ggcataacca tattgtgaga
    38821 tgagtgtatt gagagccata ggccagtttg tctccctttg gttgcctgta agcatacacc
    38881 ctgtgaatca ttcacgctga aaaaggacat acatcagacg gtgcaaatta catggatgaa
    38941 caaaaaataa tcacaagctg gcttatactt gaactgtcag gttcatgaac tcacaacaag
    39001 ggggcggtat gagccatgtc tggggactgt tttcccatcc cgatcgtgaa atgcaggtga
    39061 ttaatcgcga aaacgaaacg atttctcacc attataccca ccacgttttg ctgatggcgg
    39121 cgattccggt gatttgcgcc ttcattggca ctacacagat tggctggaat tttggcgatg
    39181 gcactatcct gaagttatcc tggtttaccg gactggcgct ggctgtcttg ttttacggcg
    39241 ttatgctggc tggggttgcg gtgatggggc gggtcatctg gtggatggcg cgcaattacc
    39301 cgcagcgtcc gtctcttgcg cactgcatgg tcttcgcggg ctacgttgca actccgctgt
    39361 ttttaagcgg tctggtggcg ctttatccac tggtctggtt gtgtgcgctg gtcggcacgg
    39421 tggcgctgtt ttacaccggc tacctgcttt accttggtat tccgagcttc ctgaatatca
    39481 acaaagagga aggccttagc ttctccagtt cgacgctggc gattggcgtg ctggtactgg
    39541 aagtgctgct caccctaacc gttatactat ggggctacgg ttatcgtttg ttctagaaag
    39601 ttctttgacg ttgcattgct ggcgtaaatg tcagcaatgc agcatttact cacgattctc
    39661 ctttgacgat cgcacttttt gctcgttatg atggcgtcgc tagcctcagt aaatccttac
    39721 tccggcggtg cgcaacccgt gcgcgtgaac cactatctga atgctcatca tgccgaaatt
    39781 tcgagtttct ttatttagct tggccctgat gctggctgtg ccttttgcac cgcaggccgt
    39841 tgcgaaaacg gcagccgcta ccaccgcttc acaaccggaa attgcctccg gtagcgcgat
    39901 gattgttgat ctgaatacca acaaagtgat ctattcgaac cacccggatc tggtgcgtcc
    39961 gattgcgtct atcagcaaat taatgaccgc gatggttgtg ctggatgcac gactgccgct
    40021 ggatgaaaaa ctaaaagtgg atatcagcca gacgccggag atgaaagggg tctattcgcg
    40081 cgtacgactg aatagcgaaa tcagccgtaa agatatgctg ttgctggcgc tgatgtcttc
    40141 agaaaaccgc gcggcggcaa gccttgcgca ccattatccc ggtggttaca aagcctttat
    40201 taaggcaatg aatgcgaaag cgaaatcgct cggaatgaac aacacgcgct ttgttgaacc
    40261 taccggattg tcggtgcata acgtttcaac tgcccgtgac ttaaccaaac tgctcattgc
    40321 cagcaaacaa tatccgttga tcgggcagtt aagtactacc cgggaagata tggcaacctt
    40381 ctctaacccg acgtatacgc tgccgttccg caatactaat catctggtgt atcgcgataa
    40441 ctggaatatt cagttaacca aaaccggctt taccaatgcg gcgggccatt gtctggtgat
    40501 gcgtacggtt atcaataata aaccggtggc gctggtagtg atggacgcgt ttggcaaata
    40561 tacccatttt gccgatgcca gccgcctgcg tacctggatt gaaaccggta aagtgatgcc
    40621 tgtgccggca gcagcgttga gctataaaaa acaaaaagcc gcccaaatgg cggcggcggg
    40681 gcagacggca cagaacgatt aagtgaattc ggatggcgat actctgccat ccgtaatttt
    40741 tactccactt cctgccagtt tttccgttta ctggttttac cgatccccgg attcatgcta
    40801 ttggtcggat cattctcgcg atagaacttc tgcaacgtct ccggtgcttt atacaaatga
    40861 ccgacgttat gctcggcagg gtactgcgcg ccgcgctgct gtagcagttc cagcatctgt
    40921 tcttttaacg catgcacatc cacgcctttt ttcactatgt aatcctgatg gaagacatag
    40981 cacataaaat ggccgtaata gagcttgtgc accagctggc tgtcgatctc cggcggtaaa
    41041 tgctcatacc actcggtgtc gttacgccgc agagcgatat ccagtgccag aatgtcttcg
    41101 acttcatcgg aatgcaccgc ctgataacga attgctgcgc ccgcagcggc gaaacggtgt
    41161 aaaaacgctt tgctaccttc ctccggcgta cagacaaaga aatcgccctc ggcctgtttg
    41221 aaataatcca ccagccacga tttggcttca ccgacgccat cgcctgccat ttttaacagc
    41281 agatgatgct cgtatttatc gcgccagttt ttcatgcgcg gcggtaaatg gctggggaac
    41341 aggtgaccga atttttgcat tgcacggtca gtaaaatgcg gacggaagaa tttcactttc
    41401 tccagcatcg catcggtgcg tcccttgaga ttaaagaaga acggcatctt gtcggtgcct
    41461 aacttatcaa tcatcaggaa ggtgtctttg ccgtattttt ccgcaatatc gtagatatcc
    41521 cggtgcatat attccccggc aaccggcaga ttttcgaagt tagccagaat atgacggcgg
    41581 atttcggtca gcacttccgg ctggttggtg ccgatataaa acacctgctg atttttttcc
    41641 gcttcgaagg tatcaagacg taccgcaaag acggccagct tcccggcgca accagaagat
    41701 tcaaataacc gatccggatc ggcgttataa cgtgcgggcg tgtcggcttc aatatcacga
    41761 acgcggtgga catagtcata atcgtgggcg tgacgaccat cgtgacgcac atcgtcatct
    41821 ttgatgcgat cgtcatccag cttgctaagg atctgctccg gcgtttcgcc cagatcaatc
    41881 cccagatggt tcaccagcgt cagtttgcca tcttcatcta tacgcgcgaa taacgacatt
    41941 tcggtatacg ccgggccacg ttgcaccagc gagccgccgg agttgttaca aataccgccg
    42001 atgaccgatg cgcctataca cgatgatcca atcaccgaat gcggttcgcg tcccagcggt
    42061 ttgagggctt tttccagcga atagagcgtg gtgcccggat aggccagcac ctgttcgccc
    42121 ttgccgagaa cgtgcagctt gtcgagacgc agggtgctga tgatcacgat atcgcgatca
    42181 tagtcgttac cgtttggcgt cgatccttcg gtcaggcctg tattggcagc ctgcatcaga
    42241 ataattttgt cggcggtgac gcaggctttc agcacccgcc acaattccag tagtgagcca
    42301 gggaaaacga cagccagcgc gtcgccctga ccagaacgga agcccttgcg atagcgggcc
    42361 gtttttgcgg gatcggtgag caggtgtgaa tgaccgacca gacgggcaag ttcattcaaa
    42421 aaggctttat tatcagttgt tgtcatggaa gacattttcc actccttgtg gtggcgaaaa
    42481 aatatcacaa actagaatag cgccttgtat gacaattcag cgagtgaata ttttgaaaat
    42541 tcagacaata aactgagcat tctttttctc tctccatcat gcttatggca aactacggtt
    42601 tttgcaccat tttcgctgcc acgtcgggca acaaaggaag aaaaatccat atgaaatggc
    42661 tatgttcagt aggaatcgcg gtgagtctgg tcctgcagcc agcactggcg gatgatttat
    42721 tcggtaacca tccattaacg cctgaagcgc gggatgcgtt cgtcaccgat ttgctcaaga
    42781 aaatgacggt tgatgagaaa attggtcagc tgcgcttaat cagcgtaggc ccggataatc
    42841 cgaaagaggc gatccgcgag atgatcaaag acggtcaggt tggggcgatt ttcaacaccg
    42901 taacccgtca ggatatccgc gccatgcagg atcaggtgat ggaattaagc cgcctgaaaa
    42961 ttcctctttt ctttgcttac gacgtgctgc acggtcagcg cacggtgttc ccgattagcc
    43021 tcggtctggc ctcgtctttt aacctcgatg cggtgaaaac ggtcgggcgt gtctctgctt
    43081 atgaagcggc agatgatggc ctgaatatga cctgggcacc gatggtcgat gtctcgcgcg
    43141 atccgcgctg gggacgtgct tccgaaggtt ttggcgaaga tacgtatctc acctcaataa
    43201 tgggtaaaac catggtggaa gcgatgcagg gtaaaagccc ggcagatcgt tactcggtga
    43261 tgaccagcgt caaacacttt gccgcatacg gcgcggtaga aggcggtaaa gagtacaaca
    43321 ccgtcgatat gagtccgcag cgcctgttta atgattatat gccgccgtac aaagcggggc
    43381 tggacgcagg cagcggcgcg gtgatggtgg cgctgaactc gctgaacggc acgccagcca
    43441 cctccgattc ctggctgctg aaagatgttc tgcgcgacca gtggggcttt aaaggcatca
    43501 ccgtttccga tcacggtgca atcaaagagc tgattaaaca tggcacggcg gcagacccgg
    43561 aagatgcggt gcgcgtggcg ctgaaatccg gaatcaacat gagtatgagc gacgagtact
    43621 actcgaagta tctgcctggg ttgattaaat ccggcaaagt gaccatggca gagctggacg
    43681 atgctgcccg ccatgtactg aacgttaaat atgatatggg gttgtttaac gacccataca
    43741 gccatttggg gccgaaagag tctgacccgg tggataccaa tgccgaaagc cgcctgcacc
    43801 gtaaagaagc gcgtgaagtg gcgcgcgaaa gcctggtgtt gctgaaaaac cgtctcgaaa
    43861 cgttaccgct gaaaaaatcg gccaccattg cggtggttgg gccactggcg gacagtaaac
    43921 gtgacgtgat gggcagctgg tccgcagccg gtgttgccga tcaatccgtg accgtactga
    43981 ccgggattaa aaattccgtc ggtgaaaacg gtaaagtgct gtatgccaaa ggggcgaacg
    44041 ttaccagtga caaaggcatt atcgatttcc tgaatcagta tgaagaagcg gtcaaagtcg
    44101 atccgcgttc gccgcaagag atgattgatg aagcggtgca gacggcgaaa caatctgatg
    44161 tggtggtggc tgtagtcggt gaagcacagg ggatggcgca cgaagcctcc agccggaccg
    44221 atatcactat tccgcaaggc caacgtgact tgattgcggc gctgaaagcc accggtaaac
    44281 cgctggtgct ggtgctgatg aacgggcgtc cgctggcgct ggtgaaagaa gatcagcagg
    44341 ctgatgcgat tctggaaacc tggtttgcgg ggactgaagg cggtaatgca attgccgatg
    44401 tattgtttgg cgattacaac ccgtccggca agctgccaat ttccttcccg cgttctgtcg
    44461 ggcagatccc ggtgtactac agccatctga acaccggtcg tccgtataat gccgacaagc
    44521 cgaacaaata cacttcgcgt tattttgatg aagctaacgg agcgctttat ccgttcggct
    44581 atggtctgag ctataccact ttcaccgtct ctgatgtgaa actttctgcg ccgaccatga
    44641 agcgtgacgg caaagtgacg gccagcgtgc aggtgacgaa caccggtaag cgcgaggggg
    44701 ccacggtagt gcagatgtac ttgcaggatg tgacggcttc catgagtcgc cctgtgaaac
    44761 agctgaaagg ctttgagaaa atcaccctga aaccgggcga aactcagact gtcagcttcc
    44821 cgatcgatat tgaggcgctg aagttctgga atcaacagat gaaatatgac gccgagcctg
    44881 gcaagttcaa tgtatttatc ggcactgatt ccgcacgcgt taagaaaggc gagtttgagt
    44941 tgctgtaatg ctttagtaag ggcgcgacgt ttgcgccctt tgcctgatgt tcatatctta
    45001 acttgctgaa ttttcgccct ttgtaggccg gataaggcgc tcgcgccgca tccggcagga
    45061 aacgccggac cgaatatgcc tcttaagtat ttatctgatt ctgcaactga atccttccgc
    45121 tcaggctaac tctgtcatta tcaactatgc ttttctctta attcgctgaa aacaacagta
    45181 aaagaatgag gaaagcatca tgccactctt aaagctctgg gcaggttcac tggttatgtt
    45241 ggcagccgtg agcctgccgc tacaggcggc ttcccccgtt aaagtcggtt caaaaatcga
    45301 taccgaaggc gcgctgctcg gcaacatcat ctttcaggta ctcgaaagcc acggcgtacc
    45361 gacggtgaat aaagtgcaac tcggaacgac tcctgtgggg cgcggggcga ttacttccgg
    45421 tgaactggat atctatccgg aatataccgg caatggcgct ttcttcttta aagatgaaaa
    45481 cgatgcagcg tggaaaaatg ccgggcaggg ctacgagaaa gtcaaaaagc tcgatgcgga
    45541 gcacaacaag ttaatctggc tgacgcccgc gcctgcaaat aacacctgga ctatcgccgt
    45601 gcgtcaggat gtggcagaga aaaacaaact cacttcgctt gccgacctga gtcgttatct
    45661 gcaagagggc ggcaccttca aactggcagc ctcggcagag tttatcgaac gcgccgatgc
    45721 gttacccacg tttgaaaaag cctatggctt taagctcggt caggatcagt tgctgtcact
    45781 ggctggtggc gacacggcgg tgacgattaa agccgctgcc cagcaaacct ctggcgttaa
    45841 tgccgcaatg gcttacggca ctgacggtcc ggtcgcggcg ctggggctgc aaaccttaag
    45901 cgatccgcaa ggcgtgcaac ctatctacgc gcctgcacca gtggtgcgtg aatcggtgtt
    45961 gaaggagtat ccgcaaatgg cacagtggct acagccagtc ttcgccagcc tcgatgcaaa
    46021 aacattgcag caactgaatg ccagcattgc agtggaagga ctggatgcca aaaaagtggc
    46081 tgccgactac ctgaaacaaa aagggtgggc gaagtaattt cccgtgactt atctacgtat
    46141 taatcctgtt ctggcgctgc tgttgttgct gacggcaatc gcagcggcgc tgccgtttat
    46201 cagttacgcg cctaatcgtt tagtctcggg tgaagggctt catctctggc aactgtggcc
    46261 gcaaacgatc tggatgctgg tgggcgttgg ttgcgcctgg ctgacagcct gttttgtccc
    46321 cgctaaaaaa ggcagcattt ttgcactcat tctggcgcaa ttcgtcttcg tattgttggt
    46381 gtggggagct ggaaaggcgg cgacccaact ggcgcaaaat ggcagtgcgc tggcgcgtac
    46441 cagcctcggc agaggtttct ggctggctgc ggcgctggca ttgctggcct gtagcgatgc
    46501 catccgccga atctccacgc atccgctgtg gcgctggttg ttgcatatgc agattgccat
    46561 tattccgctg tggttgctgt actccggcac gcttaacgat ctctcactaa tgaaagaata
    46621 cgccaaccgt caggatgtgt ttgacgacgc gctggcacaa catctgacgt tgctgtttgg
    46681 tgcggtgctg cctgcgttag tgattggtgt gccgttgggc atctggtgct acttttccac
    46741 tgcgcggcag ggggcaattt tttctctgct caatgtcatt cagaccgtgc cttcggtggc
    46801 gctctttggc ctgttgattg cgccgcttgc cgcgctggtt acggcctttc cgtggctggg
    46861 gaagctcggc atagcaggaa ccggaatgac acccgcactg attgcgctgg tgctctatgc
    46921 cttgctgccg ctggtgcgag gcgtggtagt cggtttgaac cagatcccgc gcgatgtgct
    46981 ggagagcgcc agagcgatgg ggatgagcgg ggcgcagcgt ttcctgcatg ttcagttacc
    47041 gctggcgtta ctggtatttc tgcgcagcct gcgggtggtg atggtgcaaa ctgtaggtat
    47101 ggcggtgatt gcggcgttaa tcggcgcagg cggttttggt gcgctggttt tccaggggct
    47161 gctaagcagc gccattgatt tagtgttgct gggggtgatc ccggtaattg ttctggcggt
    47221 gcttaccgac gcgctgttcg atttgcttat cgcactgctg aaggtgaaac gtaatgattg
    47281 aatttagcca tgtcagcaaa ctgttcggcg cacaaaaagc cgttaacgat ctcaatctca
    47341 attttcagga agggagtttt tcggtgctga ttggcacatc tggctccggc aaatccacca
    47401 ccctgaaaat gattaaccgc ctggtggagc atgacagcgg agtgatccgc tttgccggag
    47461 aagaaattcg ctcgctgcca gtactggagt tgcgccgccg gatgggctat gccattcaat
    47521 ctattggcct gttcccccac tggagcgtgg cgcaaaacat cgctaccgtg ccgcaattac
    47581 aaaaatggtc gcgggcgcgg attgacgatc gtatcgacga attaatggcg ctactggggc
    47641 tggagtcaaa tttgcgtgag cgttatccgc atcagctttc cggtggtcag cagcaacgtg
    47701 tgggagtggc gcgtgcactg gctgccgatc cgcaagtctt actgatggat gaaccttttg
    47761 gcgcactgga cccggtaacg cgcggcgcgt tgcaacaaga gatgacgcgc attcaccgtt
    47821 tgctggggcg cactattgtg ctggtcactc atgatattga tgaggcgcta cggctggcag
    47881 aacatctggt attgatggat cacggtgaag tggtgcagca ggggaatccg ctgacgatgc
    47941 tgactcgtcc ggcgaatgat tttgtccgcc agttttttgg acgtagtgaa ctgggggtgc
    48001 acctgctttc gttacgtagc gtggcggatt acgtgcgtcg cgaagaacgg gcagaaggtg
    48061 aggcactggc agaagagatg acgctacgcg atgcgctctc cctgtttgtc gcgcggggat
    48121 gcgaggtgct gccggtggtg aacacgcagg gccagccttg cggcacgctg cattttcagg
    48181 atctgctggt ggaggcgtaa gcgtatgaag atgttgcgcg atccgctgtt ctggctcatt
    48241 gctctttttg tggcgctgat tttctggctg ccttacagcc agccgctgtt tgctgccttg
    48301 ttcccacaac tgccacgacc cgtttatcag caagaaagtt ttgcagctct ggcactggct
    48361 catttctggc tggtgggaat ttcgagtttg tttgcggtga tcattggcac aggtgccgga
    48421 attgctgtta ctcgcccgtg gggcgcggaa tttcgcccac tggtggaaac tattgccgcc
    48481 gttggacaga cttttccgcc tgtcgcagtg ctggcgattg ccgttccggt gatcggcttt
    48541 ggtctgcaac cagcgattat cgccttgatc ctttacggtg tgctgcccat cctgcaggtg
    48601 acacttgccg ggctgggagc gattgatccc agcgtgacag aagttgcgaa aggtatggga
    48661 atgagtcgag gtcagcgact gcgtaaggtc gagctaccgc tggcggctcc ggtgattctg
    48721 gcgggcgtgc gaacttcggt gattatcaac attggtacgg cgacgatcgc ctcaacggta
    48781 ggggccagta cgctgggtac gcccatcatc atcgggctta gcggatttaa taccgcgtat
    48841 gtgatccagg gggcgttact ggtggcactg gcggcgatca tcgcagaccg cctgtttgaa
    48901 aggctggtgc aggcgcttag ccagcacgca aaataaaggt ataacctgcg agcatgacgc
    48961 caccaattcc gcctaacgcc ataaacagga acagggcgat gaccccaatt ttagctatgc
    49021 gcataatgca ctccttatgt taacgaaagg attgtacagt aaagcgcatt tgttaacgaa
    49081 tcattaaata ccgagtggga aaatatcatg gccttgttct tgccaactgg tgagttgctg
    49141 ctgttgggcg gaggttcgat tttcaccgca ccacaccagc aatgtacggc cttcgaatag
    49201 ttcagggcgt agttgattga gcgagtgggc gagcgaggac atcaatgcgc catccttgtt
    49261 gactggcaat ccagccctcc agccacagac gggtggtatc ctgaatattc cagccaacca
    49321 ccagcgcatc tttaccctgt tttttacgtg ccgaagccag acaaatggcg atgtagttga
    49381 tcagtacgcc gtcgaggatc gccagcagcg cctggagagt cggttgttgg cactgaagcc
    49441 gtcggcgcag aggaataaac agatgtgtgg tgagtgtctg ggcggggtaa tcctgaccgc
    49501 gctctttgat ccacgttcgc aggctatgta gattgc
//
LOCUS       DAEEUE010000021        48607 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.021, whole genome shotgun sequence.
ACCESSION   DAEEUE010000021 DAEEUE010000000
VERSION     DAEEUE010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48607
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.021"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            30..1280
                     /locus_tag="KLK84_001264"
     CDS             30..1280
                     /locus_tag="KLK84_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBE2008659.1"
                     /translation="MSKLESLPEFELPEITDNELLVSVISDSVCLSTWKAALLGSEHK
                     RVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEY
                     FGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHR
                     MGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLA
                     ASKGIELVYVNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLN
                     FFAGLTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHI
                     GGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETH
                     GIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            1277..2242
                     /gene="yggF"
                     /locus_tag="KLK84_001265"
     CDS             1277..2242
                     /gene="yggF"
                     /locus_tag="KLK84_001265"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HBE2008660.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPHTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2264..2773
                     /gene="fumE"
                     /locus_tag="KLK84_001266"
     CDS             2264..2773
                     /gene="fumE"
                     /locus_tag="KLK84_001266"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HBE2008661.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNETLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2770..3483
                     /locus_tag="KLK84_001267"
     CDS             2770..3483
                     /locus_tag="KLK84_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HBE2008662.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3455..4137)
                     /locus_tag="KLK84_001268"
                     /pseudo
     CDS             complement(3455..4137)
                     /locus_tag="KLK84_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
     gene            complement(4131..4808)
                     /locus_tag="KLK84_001269"
     CDS             complement(4131..4808)
                     /locus_tag="KLK84_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBE2008663.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4796..5179)
                     /locus_tag="KLK84_001270"
     CDS             complement(4796..5179)
                     /locus_tag="KLK84_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBE2008664.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5195..5503)
                     /locus_tag="KLK84_001271"
     CDS             complement(5195..5503)
                     /locus_tag="KLK84_001271"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2008665.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5504..6082)
                     /locus_tag="KLK84_001272"
     CDS             complement(5504..6082)
                     /locus_tag="KLK84_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBE2008666.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6105..6536)
                     /locus_tag="KLK84_001273"
                     /pseudo
     CDS             complement(6105..6536)
                     /locus_tag="KLK84_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6908..7927
                     /gene="epd"
                     /locus_tag="KLK84_001274"
     CDS             6908..7927
                     /gene="epd"
                     /locus_tag="KLK84_001274"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HBE2008667.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7977..9140
                     /gene="pgk"
                     /locus_tag="KLK84_001275"
     CDS             7977..9140
                     /gene="pgk"
                     /locus_tag="KLK84_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBE2008668.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9355..10434
                     /gene="fbaA"
                     /locus_tag="KLK84_001276"
     CDS             9355..10434
                     /gene="fbaA"
                     /locus_tag="KLK84_001276"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HBE2008669.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10625..11485
                     /gene="mscS"
                     /locus_tag="KLK84_001277"
     CDS             10625..11485
                     /gene="mscS"
                     /locus_tag="KLK84_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HBE2008670.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11624..12259
                     /gene="argO"
                     /locus_tag="KLK84_001278"
     CDS             11624..12259
                     /gene="argO"
                     /locus_tag="KLK84_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HBE2008671.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12352..13092
                     /locus_tag="KLK84_001279"
     CDS             12352..13092
                     /locus_tag="KLK84_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HBE2008672.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13259..14209
                     /locus_tag="KLK84_001280"
     CDS             13259..14209
                     /locus_tag="KLK84_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE2008673.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14151..15629)
                     /gene="scpC"
                     /locus_tag="KLK84_001281"
     CDS             complement(14151..15629)
                     /gene="scpC"
                     /locus_tag="KLK84_001281"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HBE2008674.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15653..16429)
                     /gene="scpB"
                     /locus_tag="KLK84_001282"
     CDS             complement(15653..16429)
                     /gene="scpB"
                     /locus_tag="KLK84_001282"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HBE2008675.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHT
                     MNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16440..17435)
                     /gene="meaB"
                     /locus_tag="KLK84_001283"
     CDS             complement(16440..17435)
                     /gene="meaB"
                     /locus_tag="KLK84_001283"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HBE2008676.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYHQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17428..19572)
                     /gene="scpA"
                     /locus_tag="KLK84_001284"
     CDS             complement(17428..19572)
                     /gene="scpA"
                     /locus_tag="KLK84_001284"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HBE2008677.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTIADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19776..20669)
                     /gene="argP"
                     /locus_tag="KLK84_001285"
     CDS             complement(19776..20669)
                     /gene="argP"
                     /locus_tag="KLK84_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HBE2008678.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21052..21711
                     /gene="rpiA"
                     /locus_tag="KLK84_001286"
     CDS             21052..21711
                     /gene="rpiA"
                     /locus_tag="KLK84_001286"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBE2008679.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21967..23199
                     /gene="serA"
                     /locus_tag="KLK84_001287"
     CDS             21967..23199
                     /gene="serA"
                     /locus_tag="KLK84_001287"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBE2008680.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23428..23487
                     /gene="ibsC"
                     /locus_tag="KLK84_001288"
     CDS             23428..23487
                     /gene="ibsC"
                     /locus_tag="KLK84_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HBE2008681.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23820..23879
                     /locus_tag="KLK84_001289"
     CDS             23820..23879
                     /locus_tag="KLK84_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBE2008682.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23980..24582)
                     /locus_tag="KLK84_001290"
     CDS             complement(23980..24582)
                     /locus_tag="KLK84_001290"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBE2008683.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24603..24786)
                     /gene="ssrS"
                     /locus_tag="KLK84_001291"
     ncRNA           complement(24603..24786)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="KLK84_001291"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24828..25157)
                     /gene="zapA"
                     /locus_tag="KLK84_001292"
     CDS             complement(24828..25157)
                     /gene="zapA"
                     /locus_tag="KLK84_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBE2008684.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25325..25903
                     /gene="ygfB"
                     /locus_tag="KLK84_001293"
     CDS             25325..25903
                     /gene="ygfB"
                     /locus_tag="KLK84_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HBE2008685.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25929..27254
                     /gene="pepP"
                     /locus_tag="KLK84_001294"
     CDS             25929..27254
                     /gene="pepP"
                     /locus_tag="KLK84_001294"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HBE2008686.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27251..28429
                     /gene="ubiH"
                     /locus_tag="KLK84_001295"
                     /gene_synonym="visB"
     CDS             27251..28429
                     /gene="ubiH"
                     /locus_tag="KLK84_001295"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HBE2008687.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28452..29654
                     /gene="ubiI"
                     /locus_tag="KLK84_001296"
                     /gene_synonym="visC"
     CDS             28452..29654
                     /gene="ubiI"
                     /locus_tag="KLK84_001296"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HBE2008688.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29728..29811)
                     /locus_tag="KLK84_001297"
     CDS             complement(29728..29811)
                     /locus_tag="KLK84_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2008689.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30102..31196
                     /gene="gcvT"
                     /locus_tag="KLK84_001298"
     CDS             30102..31196
                     /gene="gcvT"
                     /locus_tag="KLK84_001298"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HBE2008690.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKRGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31220..31609
                     /gene="gcvH"
                     /locus_tag="KLK84_001299"
     CDS             31220..31609
                     /gene="gcvH"
                     /locus_tag="KLK84_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBE2008691.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31728..34601
                     /gene="gcvP"
                     /locus_tag="KLK84_001300"
     CDS             31728..34601
                     /gene="gcvP"
                     /locus_tag="KLK84_001300"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HBE2008692.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34868..35610
                     /locus_tag="KLK84_001301"
                     /pseudo
     CDS             34868..35610
                     /locus_tag="KLK84_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(35667..37100)
                     /gene="bglA"
                     /locus_tag="KLK84_001302"
     CDS             complement(35667..37100)
                     /gene="bglA"
                     /locus_tag="KLK84_001302"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HBE2008693.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37145..37455
                     /locus_tag="KLK84_001303"
                     /pseudo
     CDS             37145..37455
                     /locus_tag="KLK84_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37619..38278
                     /locus_tag="KLK84_001304"
     CDS             37619..38278
                     /locus_tag="KLK84_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBE2008694.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38474..39454)
                     /gene="ygfZ"
                     /locus_tag="KLK84_001305"
     CDS             complement(38474..39454)
                     /gene="ygfZ"
                     /locus_tag="KLK84_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HBE2008695.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFKHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSVSRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39697..39963
                     /gene="sdhE"
                     /locus_tag="KLK84_001306"
     CDS             39697..39963
                     /gene="sdhE"
                     /locus_tag="KLK84_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HBE2008696.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39944..40351
                     /locus_tag="KLK84_001307"
     CDS             39944..40351
                     /locus_tag="KLK84_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HBE2008697.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40391..40912)
                     /gene="fldB"
                     /locus_tag="KLK84_001308"
     CDS             complement(40391..40912)
                     /gene="fldB"
                     /locus_tag="KLK84_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HBE2008698.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            41024..41920
                     /gene="xerD"
                     /locus_tag="KLK84_001309"
     CDS             41024..41920
                     /gene="xerD"
                     /locus_tag="KLK84_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBE2008699.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41945..42655
                     /gene="dsbC"
                     /locus_tag="KLK84_001310"
     CDS             41945..42655
                     /gene="dsbC"
                     /locus_tag="KLK84_001310"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HBE2008700.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42661..44394
                     /gene="recJ"
                     /locus_tag="KLK84_001311"
     CDS             42661..44394
                     /gene="recJ"
                     /locus_tag="KLK84_001311"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBE2008701.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44485..45583
                     /gene="prfB"
                     /locus_tag="KLK84_001312"
     CDS             join(44485..44559,44561..45583)
                     /gene="prfB"
                     /locus_tag="KLK84_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBE2008702.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45593..47110
                     /gene="lysS"
                     /locus_tag="KLK84_001313"
     CDS             45593..47110
                     /gene="lysS"
                     /locus_tag="KLK84_001313"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBE2008703.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47153..47701)
                     /gene="idi"
                     /locus_tag="KLK84_001314"
     CDS             complement(47153..47701)
                     /gene="idi"
                     /locus_tag="KLK84_001314"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBE2008704.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47756..47827
                     /locus_tag="KLK84_001315"
     CDS             47756..47827
                     /locus_tag="KLK84_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2008705.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47824..47949)
                     /locus_tag="KLK84_001316"
     CDS             complement(47824..47949)
                     /locus_tag="KLK84_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2008706.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47951..>48607)
                     /gene="uacT"
                     /locus_tag="KLK84_001317"
     CDS             complement(47951..>48607)
                     /gene="uacT"
                     /locus_tag="KLK84_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HBE2008707.1"
                     /translation="IIVFIESMGMFLALGEIVGRKLSSHDIICGLRVDGVGTMIGGTF
                     NSFPHTSFSQNVGLVSVMRVHSRWVCISSGIILILFGMVPKMAVLVASIPQFVLGGAG
                     LVMFGMVLATGIRILSRCNYTTNRYNLYIVAISLGVGMTPTLSHDFFSKLPAVLQPLL
                     HSGIMLATLSAVVLNVFFNGYQHHADLVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE
                     "
BASE COUNT        11666 a        12185 c        13120 g        11636 t
ORIGIN      
        1 catcgggcat tatctgaaca taaaacacta tcagtaagtt ggagtcatta cccgaatttg
       61 aactgccaga aattaccgat aatgaactgt tagtgagtgt aatttctgac agcgtctgtt
      121 tatcgacctg gaaagcggcg ttactcggta gtgaacataa acgcgtaccc gacgatttag
      181 aaaatcatcc ggtcattacc gggcatgaat gtgccggggt tattgtcgaa gtgggtaaaa
      241 atctcactgg caaatataaa aaaggccagc gttttgtatt gcaaccggcg atggggttac
      301 caagcggata ttcagcgggc tacagctacg aatattttgg cggcaatgcc acttatatga
      361 ttattcccga aatcgccatt aatttgggct gcgtattacc gtatcacggc tcttattttg
      421 ctgcggcgtc gctggcagag cctatgtgct gcattattgg tgcttatcat gccaattatc
      481 acaccacgca atatgtttat gagcatcgca tgggcgtcaa acctggcggc aatattgcac
      541 tgctggcgtg tgcgggtccg atgggcattg gcgctatcga ttacgccatt aacggtggca
      601 tacaaccgtc acgggtggtg gtggtcgata tcgacgacaa acgcctggcg caggtgcaga
      661 agctgctgcc ggtggatctg gcggccagta aaggcattga gctagtgtat gtgaatacca
      721 aagggatgag cgatcctatc cagatgctgc gggcgctgac aggagatgtc gggttcgatg
      781 acatttttgt ttatgcggcg gtgcctgctg tcgttgagat ggctgacgaa ttactggcgg
      841 aagatggctg tctgaacttc tttgccgggc tgacggataa aaacttcaaa gtgccgttta
      901 atttctacaa cgtccattac aacagcacgc acgtagtcgg tacatccggc ggttcaacgg
      961 acgacatgaa agaggcgatt gcccttagcg ccacaggaca gttacagccg tcgtttatgg
     1021 tgacccatat tggtggcctg gatgcggtgc cagaaaccgt gctcaatctg ccggatatcc
     1081 ctggcggtaa aaaactcatt tataacggcg tgaccatgcc gctcactgcc attgccgatt
     1141 ttgccgaaaa aggcaaaacc gatccgctgt ttaaagagtt ggcgcggctg gttgaggaaa
     1201 cgcacggcat ctggaatgaa caggccgaga aatatctgct ggcacaattt ggcgttgata
     1261 tcggggaggc cgcgcaatga tgtccctggc gtggccatta tttcgcgtta cggaacaggc
     1321 agcgttggct gcctggccgc acaccggatg tggcgacaaa aataaaattg atggcctggc
     1381 ggtcaccgcg atgcgccagg cattaaacga cgtcgctttt cgtggacgag tggttatcgg
     1441 cgaaggagaa attgaccatg cgccgatgct gtggattggc gaagaggtgg gcaaaggcga
     1501 tgggccagaa gtcgatatcg cggttgatcc cattgaaggc acgcggatgg tggcgatggg
     1561 gcagagcaat gcgctggcgg tcatggcttt cgctcctcga gatagcctgt tacatgcgcc
     1621 cgatatgtat atgaaaaagc tggttgttaa tcgactggct gccggggcga ttgatctgtc
     1681 tttgcctctg gcggacaact tgcgtaacgt ggcgcgggcg ttaggtaaac cgctggacaa
     1741 gctgcgcatg gttacgctgg ataaaccgcg tctgagtgcc gcgattgaag aagcaacaca
     1801 gctgggcgtg aaggtttttg ccctgccgga tggcgatgtc gccgccagcg tgctgacctg
     1861 ctggcaggat aatccttacg atgtgatgta caccatcggc ggtgcgccag aaggcgtgat
     1921 ttctgcctgt gccgttaaag cgttaggcgg ggatatgcag gccgagctga ttgacttttg
     1981 tcaggctaaa ggggattaca cggaaaatcg gcagattgca gagcaggagc gcaagcgttg
     2041 taaggcaatg ggcgttgacg ttaaccgtgt gtactcgctc gatgaactgg tgagggggaa
     2101 cgatatcctc tttagcgcta cgggcgtgac gggcggcgag ttggtgaacg gtatccaaca
     2161 gacggcgaat ggggtgcgga cgcagacatt actgatcggc ggcgcggacc aaacgtgtaa
     2221 tataatagac tccctgcatt gatggtgatc tatcggaaga ccaatggcga cgctgacaga
     2281 agatgatgtg cttgagcaac tggatgcaca ggacaattta ttttcattta tgaaaactgc
     2341 gcattctatt ttgctccagg ggatacgcca gtttctgccg tcgctgtttg tcgataacga
     2401 tgaagagatc gtcgaatatg cagtgaagcc gttactcgcc caaagcggcc cgcttgacga
     2461 tattgatgtt gcgctgcgtt tgatttatgc gctggggaaa atggataaat ggctgtatgc
     2521 cgatatcacg cacttttccc agtactggca ttacctgaac gaacaggatg aaacgcccgg
     2581 atttgccgat gatataacct gggattttat cagcaatgtc aacagtataa ctcgcaatga
     2641 gacgctctac gatgcgttaa aagcgatgaa gttcgccgat ttcgccgtct ggtcagaggc
     2701 gcgttttagc ggaatggtca aaacggcgct gacgctggca gtaacgacca ccttaaagga
     2761 attaacgccg tgaaaattga attaacggtg aatgggctga acgtgcaagc gcagtaccat
     2821 gatgaagaga tagaacgcgt gcataaactg ttgctgcgta tgctggcggc cttacagacg
     2881 gtaaatccgc agcggcgtac ggtggttttt ctttgcgctc cgccggggac gggtaaatct
     2941 accctaacca ccttctggga atatctcgcg caacaagacc cggagctgcc cgctattcag
     3001 gcgctcccga tggatggttt tcaccattac aatagctggc tggacgcgca tcaattgcgc
     3061 cccttcaaag gcgcaccaga gacatttgac gttgcgaaac tggcggaaaa tctgcgccag
     3121 gtggtggaag gggattgtac gtggccgcag tacgatcgac aaaagcatga tcctgttgaa
     3181 gatgcgttgc acgttaccgc accactcgtc atcgtcgaag gaaactggtt gttgctggac
     3241 gacgagaagt ggtgccagct tgcgcaattt tgtgatttct cgatctttat caacgcgcca
     3301 gctgctgcgt tgcgggagcg tctggtgggg cgcaagctgg cgggaggggt atcgctggca
     3361 gatgcagaag ccttttatga ccgtaccgac gggccgaatg ttcgccgggt gctggaagag
     3421 agtctgcctg caaatttgac cttaatgatg accgctacgg gtgaatatcg tctggtggat
     3481 taacgttcct gattacacca tcttccagcc gcaccacacg agagaaatga cgccgggtaa
     3541 atgcggcgtc atgactgatt gctaccacgc tcgtgcctcg ctggcggcac ttctccagcc
     3601 agctttcaaa cacgctcaac caattttcgt caaaatcccg actcggctcg tccagcagta
     3661 atagtggcgg agagagtgct tccaggcagg cgacggcgac catccgtcgc tgcgcactat
     3721 gcagatccaa cgggtgcgag ttggcagtgt ctgctaactg acaacattgc agggcagcat
     3781 gggtacgttg agtgatttca tccgccgggc atttttgcag tttcaaacca aatgcgatct
     3841 cgtcagcgac ggtgctgtga aaaagttggt tttccgcttc ctgaaacaga acagtacgcc
     3901 aacctttgcc gcgcgctggc ggtttttcag gtttttcatc gcctgttgtt gcagcataac
     3961 cgtaccggct gtgggcgtaa gaagacctgc cattactcgt aacagcgtcg atttccctgc
     4021 gccattgtca ccggtcagcg ccaaccattc accttgtttg agttgcagag aaatgtcgca
     4081 aagacaatcc gttgccgcgc caggccagcg ataagatatc ttatttaacg ttaacatagt
     4141 ggctggagcg ctccgttttg caattgccac gcttgctggc agtagttcag gaaaggtgtg
     4201 cgatgacgtt caaaaagaat aatcagcgaa ccacgttcaa acgcccagtg ttgtaagcgc
     4261 tgcaacagca tttcgctggc ctgcggcgtc aggcggctaa aggcttcatc aagaattaag
     4321 agctttggct gcatcgcgat ggcgcaggcg atgatcacgc gctgtgtctc tccaccggag
     4381 agtgttgccg ggtgacgatg gcgcaacggc tggcactcgg tcagagtcag ggcggcatca
     4441 atccgcgcca taatctctgc ttcagcaaga cacagatttt ctggcccaaa ggcaacttct
     4501 tcttccacgc taaaggtaca gccggagagc tgcaaatagg gcgattgctg gacgagctgg
     4561 atggtggcgg attgttcatt aagcgggaga tgcccaatgg gcgtaccgag gagcgtcccc
     4621 gtgcctgtta tctctcttgg cagaaagtcc ggataccagc ccgccatcag ctgtgccagc
     4681 gtacttttgc cactgccgtt gtcaccaaag atggcgacca tccccggttt gacatagtga
     4741 aagtcataac agaacggagg atgcgttgag tgtgtcgggc aataacggaa ctgttctaac
     4801 gtaaccatat ccacgctccg aattctgtca gcatcagcaa gatcatggtg taacgcgcta
     4861 cgcgttgtag ggtgctatcg gctggtgccc acaacgttgt tcgtcggttg tggatccgaa
     4921 atgcccgcat atccagcgcc gcaccacgga cagccagatc gttaagcgca ttgtgtgtta
     4981 gcggaataat taacgcgggc atagcacgta aacgctgata ccagccctca tccagcggca
     5041 cgccgcgggc acgctgcgct tcatgaataa tcgctaattg ccgttttaac tgctcaacga
     5101 ccaataacgg cccggcaaac agataagcga cgcctggcgg caggcgagaa gcaaacagag
     5161 cgcgaataaa acattgcacg gggacgtact gcattcacaa ctgtgaggtt gaaacaatcg
     5221 ccagaatcag caaccagagc gtaatagcat gcgaccaccg ttctggcgag cgtggcgtgc
     5281 cgcttagcca ttctggtagc cagccaccgt gtaccagcca cagcccggct cccagcgaga
     5341 acatcagcca gacgacatat tttgcccgcc gccgcgtggc ctttagcgca ataaggcaga
     5401 aaaaggtcgc cgcgctatag atcggcaaaa tggtctgcgc gggcaggata agcgtggtgc
     5461 aggccgccaa tgcccacagt gttaatgagg taaatggatg catttagcgc acggcagcca
     5521 ttgccggaaa atggcgcgtg gtgcgaatcg gcagctggcg cagtaaaagc cagacaatca
     5581 ccgctgtaag aattttatcg accaggttcg cgcctatcac cgtaatcgcc accgactcca
     5641 ccaggttttg ccccatcgag tgcatccagg caacgaacaa atcggctccg ctaccggtga
     5701 caccgccgaa cagcgcagta cgcagcggta cagcgacgac cgttaccgca agcgtaataa
     5761 tcacgccgct gacaacgact tttggcaggg tgcgaaacca acccgctcgc gccagccaac
     5821 cggagaccag gccgatgacc atcgcgacgg gtgcaaatgc ggcggcgatc gggtcggtca
     5881 gcaatcccca gagtaaattg gtcagtaagc cggtcagcat tccgatcacc ggaccaagca
     5941 gtaccgcgct aataagtgtg ccgatggagt cgagaaatat aggtaatttg accatgctgg
     6001 ccagttgccc gccgatcata ttgatggcga tagatataac gatcaacacc agtgcctgac
     6061 tggaaaagtg gctacgcgcc ataagaaaac ctctttgaaa gtagttattt aacgggggaa
     6121 atatgcaact cgtcgtaacc agaaagcgcg caaggttggc ggctaaacgc cagttcctca
     6181 aggctaccga tcaccagttc aagcgtggtc cgcacggtac agccgggcat catatgaccg
     6241 ccaagcatcg tgccgtgtgg atcggaaacg cagagatgaa gatgctcacc actctgttcc
     6301 agcgtgccgt ttagtgagat cacttcgaat gtaccgttca ggagcgtggt gccttcttgc
     6361 ccggcatagc gcaaggcgac gtcagtcagg ctaccggtac agcctgcaat ctaggcggca
     6421 tgaagttgat gttgctgtgc aaaagcgtgt agctgggaaa aaacttcctg cccggggagg
     6481 aggcgcagtg cgtaaaagcg tgcggaggaa gaatatgagt agggcaatgt catcattgga
     6541 gtactccgaa actcggacgt attgcggata atatcgacgc tgcatcagtc atattctgat
     6601 ataagtcagg gtttgtgacg gcacaacgaa aagattttgc tacgcttatt cgtgattctg
     6661 caaagtggaa taaattccac aggggcgggg ctgaaaacgt gacgtaagtc agataattgc
     6721 tttctttgac tggacaatca ttccttttat tccacgtttc gcttatccta gctgaagcgt
     6781 ttcagtcgat taaatgttcg acaattaacc aatcagtcgc agtttgcgac aggtaaggtt
     6841 tccccggacg atttgctgga ttactctgtc tggcaaattt gctcagggaa aaccttgcag
     6901 gagatctatg accgtacgcg tagcgataaa tggcttcggt cgcatcgggc gtaatgtggt
     6961 tcgtgctttg tatgaatccg gacgccgggc ggaaattacc gtggtggcaa tcaacgaact
     7021 ggcggatgct gcgggcatgg cgcatttgtt gaaatatgac accagccatg gccgttttgc
     7081 atgggaagta cgacaggaac gcgatcaact ttttgttggt gatgacgcca tccgcgtatt
     7141 gcatgaacgt tcactgcaat cgctcccctg gcgtgaactt ggcgttgatg tagtcctcga
     7201 ctgcaccggc gtatatggct cccgcgagca tggcgaagcg catattgccg ccggggccaa
     7261 aaaagtgctc ttttcacatc ctggcagtaa cgatctcgac gcgaccgttg tttacggcgt
     7321 caatcaggat caacttcgtg cggaacaccg catcgtttct aacgcttcct gtaccacgaa
     7381 ttgcataatt cccgtcatca aattgttaga tgatgcgtac ggtattgagt ccggcactgt
     7441 gaccacaatt cactccgcca tgcacgatca acaggttatt gatgcatacc atcctgacct
     7501 gcgtcgcacc cgggcagcca gccagtcgat cattccggtc gatactaaac tggccgctgg
     7561 tatcacacga ttttttccgc aatttaacga tcgctttgaa gcgattgcgg tacgtgtgcc
     7621 aaccataaat gtgacggcaa tcgatttaag cgtgacggtg aagaaacctg taaaagccaa
     7681 tgaagtcaac ctgttgctgc aaaaagcagc acaaggtgca tttcatggta tagttgacta
     7741 tacggaattg ccgttggtct ctgtagattt taaccacgat ccgcacagtg ccattgtcga
     7801 tggcacccaa acccgggtca gtggcgcaca cctgatcaaa acgttggtct ggtgcgataa
     7861 cgaatggggc tttgctaacc gaatgctcga cacgacgtta gctatggcta ctgttgcttt
     7921 caggtaagac gcaagcagcg tctgcaaaac ttttagaatc aacgagagga ttcaccatgt
     7981 ctgtaattaa gatgaccgat ctggatcttg ctgggaaacg tgtatttatc cgtgcggatc
     8041 tgaacgtacc agtaaaagac gggaaagtaa ccagcgacgc gcgtatccgt gcttctctgc
     8101 cgaccatcga actggccctg aaacaaggcg caaaagtgat ggtaacttcc cacctgggtc
     8161 gtcctaccga aggcgagtac aacgaagaat tctctctgct gccggttgtt aactacctga
     8221 aagacaaact gtctaacccg gttcgtctgg ttaaagatta cctcgacggc gttgacgttg
     8281 ctgaaggtga actggttgtt ctggaaaacg ttcgcttcaa caaaggcgag aagaaagacg
     8341 acgaaaccct gtccaaaaaa tacgctgcac tgtgtgacgt gttcgtaatg gacgcattcg
     8401 gtactgctca ccgcgcgcag gcttctactc acggtatcgg taaattcgct gacgttgcgt
     8461 gcgcaggccc gctgctggca gctgaactgg acgcgctggg taaagcactg aaagaacctg
     8521 ctcgcccgat ggtggctatc gttggtggtt ctaaagtatc taccaaactg accgttctgg
     8581 actccctgtc taaaatcgct gaccagctga ttgttggtgg tggtatcgct aacaccttta
     8641 tcgcggcaca aggccacgat gtgggtaaat ccctgtacga agctgacctg gttgacgaag
     8701 ctaaacgtct gctgaccacc tgcaacatcc cggttccgtc tgatgttcgc gtagcaaccg
     8761 agttctctga aactgcaccg gctaccctga aatctgttaa cgatgtgaaa gctgacgagc
     8821 agatcctgga tatcggtgat gcttccgctc aggaactggc tgaaatcctg aagaatgcga
     8881 aaaccattct gtggaacggt ccggttggcg tgttcgaatt cccgaacttc cgcaaaggta
     8941 ctgaaatcgt ggctaacgct atcgcagaca gcgaagcgtt ctccatcgct ggcggcggcg
     9001 acactctggc agcaatcgac ctgttcggca ttgctgacaa aatctcctac atctccactg
     9061 gcggcggcgc attcctcgaa ttcgtggaag gtaaagtact gcctgcagta gcgatgctcg
     9121 aagagcgcgc taagaagtaa aaaatcacag ggcagggaaa cctgcacttg tttcagcgcg
     9181 cttttagagc acgcattttt tcatactcta aataattcga gttgcaggaa ggcgacaaga
     9241 gagtgaatcc ccaggagctt acataagtaa gtgactgggg ggagcgaacg cagacgcagc
     9301 acatgcaact tgaagtatta tgagtataag gcccgacgat acaggacaag agacatgtct
     9361 aagatttttg atttcgtaaa acctggcgta atcactggtg atgacgtaca gaaagttttc
     9421 caggtagcaa aagaaaacaa cttcgcactg ccagcagtaa actgcgtcgg tactgactcc
     9481 atcaacgccg tactggaaac tgctgctaaa gttaaagcgc cggttatcgt tcagttctcc
     9541 aacggtggtg cttcctttat cgctggtaaa ggcgtgaaat ctgacgttcc gcagggtgct
     9601 gctatcctgg gcgcgatctc tggtgcgcat cacgttcacc agatggctga acattatggt
     9661 gttccggtta tcctgcacac tgaccactgc gcgaagaaac tgctgccgtg gatcgacggt
     9721 ctgctggacg cgggtgaaaa acacttcgca gctaccggta agccgctgtt ctcttctcac
     9781 atgatcgacc tgtctgaaga atctctgcaa gagaacatcg aaatctgctc taaatacctg
     9841 gagcgtatgt ccaaaatcgg catgactctg gaaatcgaac tgggttgcac cggtggtgaa
     9901 gaagacggcg tggacaacag ccacatggac gcttctgcac tgtacaccca gccggaagac
     9961 gttgattacg catacaccga gctgagcaaa atcagcccgc gtttcaccat cgctgcgtcc
    10021 ttcggtaacg tacacggtgt ttacaagccg ggtaacgtgg ttctgactcc gaccatcctg
    10081 cgtgattctc aggaatatgt ttctaagaaa cacaacctgc cgcacaacag cctgaacttc
    10141 gtattccacg gtggttccgg ttctactgct caggaaatca aagactccgt aagctacggc
    10201 gtagtgaaaa tgaacatcga taccgatacc caatgggcaa cctgggaagg cgttctgaac
    10261 tactacaaag cgaacgaagc ttatctgcag ggtcagctgg gtaacccgaa aggcgaagat
    10321 cagccgaaca agaaatacta cgatccgcgc gtatggctgc gtgccggtca gacttcgatg
    10381 atcgctcgtc tggaaaaagc attccaggaa ctgaacgcga tcgacgttct gtaagtcatt
    10441 cctttctgct tatctcaagg cccgctctgc gggtcttttt ttcgccaaaa gcaaggtgat
    10501 tcagatgaga aatctgtgat ctatttggca aaattatgct ttattgttta cccttgtcag
    10561 actgcccgtc ataaggcggc ggagtgtatt tctccatttt gagtcagttg aaaaggaata
    10621 ttgaatggaa gatttgaatg ttgtcgatag cataaacggc gcgggaagct ggctggtagc
    10681 taaccaggcg ctgctgctaa gttatgcagt aaacatcgtg gcggcactcg cgatcatcat
    10741 cgttggtttg attatcgcgc ggatgatttc caacgcggtg aatcgcctga tgatctcccg
    10801 taaaatcgat gccactgttg ctgattttct ttctgcatta gtccgttacg gtattatcgc
    10861 ctttacgcta atcgctgccc tgggacgcgt gggtgtacaa acagcgtcag tcattgctgt
    10921 actcggtgcc gcaggcttag ctgttggtct ggctttgcag gggtcacttt ctaacctggc
    10981 cgctggcgtg ttacttgtca tgttccgccc gttccgtgcc ggagaatatg ttgacctcgg
    11041 cggcgtagcc ggtactgtgc tgagtgtgca gattttctcc accaccatgc gtactgcaga
    11101 cggtaaaatt atcgttattc cgaacggtaa aattattgcc ggaaatatta ttaacttctc
    11161 ccgcgagcca gttcgccgta acgaatttat tattggcgtg gcgtatgatt ccgatatcga
    11221 tcaggttaag cagatcctga ccaatattat ccagtctgaa gatcgcattt tgaaagatcg
    11281 cgaaatgact gtgcgcctga acgaacttgg tgcatcgtcg attaatttcg tggtccgcgt
    11341 ctggagcaac agcggcgatc tgcaaaacgt gtactgggat gtgctggagc gtattaaacg
    11401 tgaatttgat gccgccggta tcagcttccc gtacccgcaa atggatgtga actttaagcg
    11461 ggtgaaagaa gacaaagctg cgtaatcaac gctgaggcca gataatactc tctggcctct
    11521 ctcttattag ttattctgat tgccaattaa tattatcaat ttccgctaat aacaatcccg
    11581 cgatatagtc tctgcatcag atacttaatt cggaatatcc aacgtgtttt cttattactt
    11641 tcaaggtctt gctcttgggg cagctatgat tctgccgctc ggcccacaaa atgcttttgt
    11701 gatgaatcag ggcattcgtc gtcagtacca cattatgatt gccttacttt gcgctatcag
    11761 cgatttggtc ctgatttgcg cggggatttt tggtggtagc gcgttattga tgcagtcgcc
    11821 gtggttgcta gcgctggtca cctggggcgg cgtagtcttc ttgctctggt atggttttgg
    11881 cgcttttaaa acagcgatga gtagtaatat tgagttagct agtgccgaag tcctgaagca
    11941 aggcagatgg aaaattatcg ccaccatgtt ggcagtgacc tggctgaatc cgcatgttta
    12001 cctggatact tttgttgtac tgggcagcct tggcgggcag cttgacgtcg aaccaaaacg
    12061 ttggtttgcg ctcgggacaa ttagcgcctc tttcctgtgg ttctttggtc tggctattct
    12121 cgcagcctgg ctggcaccgc gcctgcgcac ggcaaaatca cagcgcatta tcaatctggt
    12181 agtgggatgt gtcatgtggt ttatagcctt gcagctggcg agagacggta ttgctcatgc
    12241 acaagccttg ttcagttagg cgctatctga tggaaaaata aaacagaggc gctaagcttg
    12301 cctccagagg tcctgaattt tgggcaagtc aattaacacg gagagactaa cgtgaagttc
    12361 aaagttatcg ccctggcggc attaatgggt attagcggga tggcagcgca ggctaacgaa
    12421 ttgccggatg gaccgcatat tgtcacctcc ggtacggcaa gtgtggatgc ggtgccagac
    12481 attgccactc ttgcgattga agttaacgtg gccgcgaagg atgccgctac agccaagaaa
    12541 caggcagatg agcgcgtcgc acaatacatt tccttccttg aactcaatca gatcgcgaaa
    12601 aaagatatca gctcagcgaa tttacgcacc cagccagatt atgactatca ggatggtaaa
    12661 agtatcctga aaggctaccg agctgtgaga acggtggaag tcacgctccg tcagttagac
    12721 aaactgaatt ccttgctgga tggcgcgctg aaggcgggtc ttaacgaaat tcgttctgtg
    12781 tcgctgggcg tggcgcagcc ggatgcctat aaagacaaag cgcgtaaggc agcgattgat
    12841 aacgcgattc atcaggcgca ggaactggcg aacggctttc atcgtaaact ggggccggta
    12901 tatagcgtgc gctaccatgt ttccaactat cagcccagcc caatggtgcg gatgatgaaa
    12961 gccgatgccg cgccggtgtc tgcccaggaa acttacgagc aggccgctat tcagtttgat
    13021 gatcaggtcg atgtggtctt ccagttagaa cctgtggatc aacaacccgc taaaacacct
    13081 gcagcacaat aatacttgta gcccgacgta ttcgtcgggc taatgccttt acccgatatt
    13141 gcctcctttt tctgtaattt tctgtaaaac catgacgtaa tcacctatct ttgctcgcgg
    13201 tttcatcaag gataggctga ctgaaacggt taagcggaca aggatgcggg gaggggggat
    13261 ggacattttt atttcgaaaa agatgcgcaa tttcatttta ttagcgcaaa ccaataatat
    13321 tgcccgggcg gcagaaaaaa tccatatgac ggcttcgcca tttggtaaaa gcattgccgc
    13381 gctggaagaa caaattggct atacgctatt tacccgcaaa gataataata tcagcctcaa
    13441 taaagccggt caggaactgt atcaaaaact gttcccggtt tatcaacgac tttctgcgat
    13501 tgataatgaa atccataact cagggcgtcg ttcacgagaa attgtgattg gcatagataa
    13561 tacttatccg acaattattt tcgatcagtt gattagtctc ggagataagt atgaaggcgt
    13621 gactgcccag ccagttgagt tcagcgaaaa cggcgttatc gataatcttt ttgaccgtca
    13681 gttagatttt attatctccc cacagcatgt atctgcacga gttcaggaac tggaaaacct
    13741 gactatcagt gaattaccac cgctgcgatt gggctttctc gtttctcgcc gttatgaaga
    13801 gcgtcaagag caagagctgc ttcaggagct gccctggcta caaatgcgct ttcaaaaccg
    13861 ggctaacttc gaagcaatga ttgatgcgaa tatgcgacct tgtgggatca atcccaccat
    13921 tatttatcgt ccctacagtt ttatggccaa aatcagcgcc gtggagcgcg gacattttct
    13981 gacggtgatc ccgcattttg cctggcgact ggtgaatccg gcaacgctga aatattttga
    14041 tgcgccgcac aggccgatgt atatgcagga atatctttat tcaatcagaa atcatcgtta
    14101 taccgccacg atcttcagca tattgctgaa gatcgtgacg ggacaaacca ttaacccagc
    14161 atcgagccgg ttgcaattaa attacggtgt aagtcgaaga cgtggctaag atcgtggtga
    14221 atatgtccgc cgggcgcatt ttccagatag cgatgcagat aatcccgata cataggatgt
    14281 gcacaattat caatgatagt gcgggcgcgt tgaagcgggg aaagaccgcg cagatcggcg
    14341 atcccttgtt cggtgatgat cactttgacg ctgtgttcgc tgtgatcaac atggctgcac
    14401 attggcacga cggttgagat cttgccttct ttagcaatcg acggagccat aaagatcgac
    14461 agatacgcgt tgcgttcaaa atcaccgctg ccgccgatgc cgttcatcag atcgaccccg
    14521 gctacgtgcg ttgaatttgc atgcccgtaa atatcaaact ccaggccgac gttcagagcg
    14581 atgacgccca gacgacggat gatttccggg ttattggaaa tctcctgcgg acgcaacaca
    14641 atgcggctgg caaagtaatc catattgtcg taaatcttgc gcagggaatc ggccgagatt
    14701 gtcaggctgg aggcgctggc cccgctgatt ttgccggttt ccagtaaatg caccaccgat
    14761 tcctgtagca cttccgaata catcatgaac ggaggaattt ccgggttttc ccccagacgt
    14821 gccattaccg cattattgat attgcccacg ccactttgca gcggcagaaa ttccggcgga
    14881 atacgcccat gcgccatttc ctgcaataag aacgtgacca cgttatcggc aatctgctgg
    14941 cacatgggat tttgcttatc cagcatatta ccggcgtcgg gcaagttggt ttccacgacg
    15001 gcgacaatct ttttcggatc gatttgcaca tagcgggtac cgacgcgatc cattgcatgg
    15061 aagatcgaca cgctattgcg ccgcggtggc gcgccaggaa tcacaatatc tgccagttct
    15121 gcaacgcgcg gatcgtgata gtgattaagt tcaatgatca ctttcttcgc tcgcagcagc
    15181 caggtcggcg cattaccgat cccgctggtt aaccagactc gaccatccgg tgccagtgcc
    15241 gatgcttcaa tgacggcaac atcaatgtcg ccgaagaaac cgtaattgac catttgcgcc
    15301 acttcgctca aatgcaggtc aacgaaactc accgcgccct gattgatctt tttacgtaaa
    15361 ctggacgatg tttgatatgg cgcacgccag gaaacagcat cggcgtcaga aagtacatcg
    15421 tcagcggcgg cgctgattga cgcccccgtc agcaggcgaa tttgatacgg ctttttggcc
    15481 tcatgctgtt cgttagctct gcgggcaatc gcggcgggta gggctttcgg cgaacccgct
    15541 ggggtaaagc cgctaaatgc caccatgtcg ttatgctgga taatttctgc cgcttcatcg
    15601 gcggtcatcc ttgtccactg agtttccatt tttactcctt cgttcgcagg gattaatgac
    15661 caacgaaatt aggtttacgt ttttcgagga aagcgttcat cccttcctgg taatcttcgc
    15721 tgtcataaac cgcgcggcgc atcccctgaa tacgttcaaa ttcatcggag ttcatggtgt
    15781 gtgcttcgcc cagtacacgc agctcttctt tgataacggc aatggctaac ggcgctttct
    15841 cagagatgtg gtgcgccatt tgtaaggtga aatcttccac ttccacaaca tggttgagga
    15901 tgccgacagc cagcgcgcgc tgggcggtga ttggcgaagc ggtaaaaatc agctctttga
    15961 caatgtggaa gcccgcgtcg cgggtcaggt tgtgaatgcc gaccaggtta tacgggacgc
    16021 cgaggtttac aggcgtcatt gagaaggttg aggtactggc ggcgatgatc agatcggaac
    16081 tcatgatcat ttcaaatgcg ccaccccaaa cactaccttc caccatcgaa atgatcggtt
    16141 tcgggaattt ttggatcatg cgggtgattt gacgcaatgg atcatcatag gagagcggat
    16201 cgcgaccgcc agacggcagt tcgtgaatat cgtgacctgc ggagaagact ttggatccac
    16261 ttggcgcccg caaaatgata cagcgaattt ccggccggtt gagatcgctt aacgcctgca
    16321 taagatcatc aataaagact ttacttaagg cattaagttt tcgtccatag ttaaactcaa
    16381 tgaccgccac tttgttgata gtggcaacgt tcacatactg ataagacata aaaattcctt
    16441 taatcaaaat attgcgtctg gataaattca ctgagctgcc gcaggccggt gcgcggtgag
    16501 agcgtattgt ttttgaccgc taaaagcgtc tggtggtaat agcgatcgaa atcttcattc
    16561 gcgaacaggt gattcagtac ttcttcttcg gtctgcttac gcagccattc caccgattgt
    16621 tgttgccgca cttgttgtaa acgaccactg gcagttagcg cggttttgaa gtcgatgatg
    16681 gcgtgccaga tctcatcgat tccacgtttt tccagtgcgc tacaagtcag accccgtggc
    16741 tgccattcgt cgtatttacg tcgcagaata tgcagggcac tctcgtacat atgccgggca
    16801 atggcgacat tggtatggtt atcgccatcg tctttgttga taacgagcag atcagccact
    16861 tccatcagcc cttttttaat gccctgcaga tcatcgccgc caccggcaat ttgcaacgag
    16921 ataaaacagt ccaccatgcg ggcgacttcc gtttccgact gcgcgacgcc aaccgtttcg
    16981 acaatcacta cgtcataacc cgccgcttcg cataacagca ttaattcccg tgctcgctga
    17041 ctggcaccgc ccagatgacc ggaggagggt accgggcgaa taaacgccgc ctcggcacgc
    17101 gccaggtcat tcatgcgggt tttatccccg agaatgctac cgccagtgac cgggctgctg
    17161 ggatcgaccg caataacagc aacctttaat ccctcacgaa tcaacaacat gccaaaggct
    17221 tcaagaaagg tacttttccc cgcgccgggt gtgccggtaa cgcccagtcg cagggcgtta
    17281 ccacagaacg gcataatagc gtcaagcagc tgtgtactta gcgcctgatg acgtgggtga
    17341 cggctttcca ccagcgtcat ggcttgggcg agcgtggcac gctcaccctg acgtaagcgg
    17401 cgaatacttt ctgccagcgt ggcttcatta atcatgatgc tggcttatca gattcagtac
    17461 gtcgcgcaca ctgtcgagca taggtgtacc tggaccataa atcgccgcca cgccgcgctc
    17521 ttgcaggaag gcgtaatcct gcggcggaat gacgccaccc gcgaccacgc agatatcttc
    17581 gcgtccccat tttttcagcg cttcgaccag ttccgggatc agcgttttat gaccggcagc
    17641 cagtgaggat gcgcccacta cgtgaacgtc gttttctacg gccaggcggg cgatctcttc
    17701 aggtgtagag aacatcgggc ttaaatctac gtcgaaaccg agatcggaat aggcgctggc
    17761 gatcactttc gcgccgcgat cgtgtccatc ctggcccatc ttagcgatca gaatgcgcgg
    17821 gcgacgacca ttgtcggcaa ggaactgctc cgtttgcgca acaatggcat cgaactcgga
    17881 ggccgatttc tcagactgat gatagctttg cgcaatcacg ccggtaacac actggcttgg
    17941 caccagataa cggtcgaaag cgacttccag cgcatcggaa atttcaccca gggtggcgcg
    18001 aacgcgagcg gcattaacag cggcagccag caggttttcg ttatgctgtg cggcgtgagt
    18061 cagggcgttc aacgcggcgg ttacggcggc atcatcacgg gtggcgcgaa tgcgttccag
    18121 cgaagcaatt tgctcgttac gcaccatcac gttgtcgatc tcaagtacat cggtttcgtc
    18181 ttcgtgatcc agtttgtact tgttgacacc aacgatgaca cgcttgccct ggtcgatcag
    18241 cgactgttcg cgcgctgagg cctcttcgat cattcgtttt ggcagacctg cttcgatcgc
    18301 tttcgccatg ccaccggctt cgtcgatctg ttggataata gctctggctt gtttgacgat
    18361 ttgatcggtc agcgactcaa tgtaatagga tccggccagt ggatcgacgg tgtggcagag
    18421 ttctgattct tcctggatga tgatctgggt gttgcgggca atgcgtgctg agaaatcggt
    18481 aggcaaacca agcgcttcgt caaaggcgtt ggtatgcagt gactgagtac cgcccagcgt
    18541 cgcagccagt gcttcaatgg tggtgcggat aacgttgtta tacggatcct gttcagtcag
    18601 gctccagcct gaggtctggc agtgggtacg cagcgccagt gatttcggat cctgtgcgcc
    18661 aaatccactg accgcttcgc tccataaata acgtgccgca cgcaacatgg cgacgttcat
    18721 aaacagatcc atgccgatac cgaagaataa cgacaggcga ggagcgaagt catcaatttt
    18781 cagtccggca gagattgctg ctttgatgta ctcaatccca tcggcgatcg taaatgctac
    18841 ctgctgcacg cagttggcac ccgcttcccc catgtggtaa ccgctgatac tgatggtatt
    18901 aaatcgcggc atgttgccgg aacaccaggc gatgatgtcg gcgataatgc gcattgacgg
    18961 ttttggtggg taaatatagg tgttgcggca gaggtactct ttgagaatat cgttttgaat
    19021 ggtgccggtc agtttatcag gtgtaacacc ttgctcttct gcggcgacga tataaaacgc
    19081 cagtactggt agcactgcgc cattcatggt catcgaaacc gacattttat ccagcgggat
    19141 ctggtcgaac aggactttca tatcttccac ggtgtcgata gcgacgcccg ctttgccgac
    19201 gtcacccgcc acgcgtgggt tatcggagtc gtagccacgg tgggtggcaa ggtcaaacgc
    19261 aacggaaaga cctttttgcc cggcggccag gttacggcga taaaaagcgt tggactcttt
    19321 tgctgttgaa aaaccagcat actgacggat ggtccacggt tgggcggtat acatagtggc
    19381 acgcgggcca cgaacgtagg gcggcaaacc aggaagggta cctgtcacct ccagattatc
    19441 gagatcggct tcggtataca gcggcttgat ggcgatccct tccgcggttt gctgaaccag
    19501 cgagtcgaca gttttctccc gacggctcaa ttccttgttg gcaagctgtt gccactcctg
    19561 ctcgttagac attatttgct ccgtaaaacg caatcaaaat gagggtattt gcgcttacgg
    19621 tgagcttttt acgtgggttt gtcgccagca aaaaaaatgc attcagacgt cgttttttgc
    19681 cgcctgagat ttgtctattt tgtgatgtga ttcaaggaat acaaaaagaa gtgaggacaa
    19741 cgcctgatat gtgctatcag gcattatttg atggattaat cctgacgaag gactttgtga
    19801 ccataatcga gtaacgcatc agtgacttta cgcatcatgc ggctttcagg agcaaagcgg
    19861 tgccagtaga gcatccgtcg ttgaaatagc ccaggcgtta agtcaatcag ttcaccgctg
    19921 gccagctctt tctcgatttg caggtgcggg atcatacagc aggtggtgcc ctggcgagca
    19981 agttgtacga acgcttctga agaattaacg atatggcagg gtacgctgcc aggaggaaga
    20041 tcgaagtttt gctgcaaaaa ggcctggtgc atatcgtcaa gatggtcaaa cgcgaccact
    20101 ggcgctttca ataatgccga acgcgttacg ccgttaggaa aatatttttc ggcaaacggt
    20161 tttgagctga cgaacagata gtcgagcgca ccgagtttat cgacaagaca actcggcagc
    20221 gcctgatgtt gaatactcac cgcgccgacc acttcgccgc ggcgcagacg ttcctgagta
    20281 cgggtttcat cttctacctg caagttgagg cggataggcg aatcagccaa cacaggagcc
    20341 agtgccggaa gcaaccacgt cgccagactg tcggcgttga ccgccagtga aagcagcagc
    20401 ggagtcgaac cggtttgttc atcgcccagc cactcttctt ccagcaactc cacctggcgc
    20461 agcagtgcca gcagtttttg cccttgttcc gtcgggcgcg gcggtacggt acgcaccaac
    20521 agcggctgcc cgaacatatt ttccagttgc ttaatgcgct gtgagacggc tgattgtgta
    20581 atgcacagct tttgtgccgc gcgctcaaat cctcgttcac gtatcaccgc atccagtgcc
    20641 tgtaatgttc tgtagtccgg gcgtttcatt gctgttgtct gctcctggta aatgcttttt
    20701 cctgcactat gacacaattt tgtgtcaggt tgcatatacc gaagccgtcg gcgggtaaca
    20761 tttgtatcaa tggttaaacg cgcagcttaa aagatcaggg atttgcgcta ttgcatttcg
    20821 cttagcgact tcgcgccctg gttatgttga atggcgtggc gttattgcct ctgttcgcac
    20881 cgtggagcgg catacccaga ccgttgtatg ctttatttgc ggggcgaaag gggatgcccg
    20941 ccattgcgcg atattttatg gatgagttaa ccacccagct tgccaacggg gtctgaatcg
    21001 ctttttttgt atataatgcg tgtgaaattt cataccacag gcgaaacgat catgacgcag
    21061 gatgaattga aaaaagcagt aggatgggcg gcacttcagt atgttcagcc cggtaccatt
    21121 gttggtgtag gtacaggttc caccgccgca cactttattg acgcgctcgg cacgatgaaa
    21181 ggccagattg aaggggccgt ttccagttca gatgcttcca ctgaaaaact gaaaagcctc
    21241 ggcattcacg tttttgatct caacgaagtc gacagccttg gcatctacgt tgatggcgca
    21301 gatgaaatca acggccacat gcaaatgatc aaaggcggcg gcgcggcgct gacccgtgaa
    21361 aaaatcattg cttcggttgc ggaaaaattt atctgtattg ccgacgcctc caagcaggtt
    21421 gatattctgg gtaaattccc gctgccagta gaagttatcc cgatggcacg tagtgcagtg
    21481 gcgcgccagc tggtgaaact gggcggtcgt ccggaatacc gtcagggcgt ggtgaccgat
    21541 aatggcaacg tgatcctcga cgtccacggc atggaaatcc ttgacccgat agcgatggaa
    21601 aacgccataa atgcgattcc tggcgtggtg actgttggct tgtttgctaa ccgtggcgcg
    21661 gacgttgcgc tgattggcac acctgacggt gtcaaaacca ttgtgaaatg atctgacggg
    21721 ggaacctccc ccgttaaaaa aattctcttc attaaatttg gtgacatgtg tcacgctttt
    21781 accaggcaat tgtcgattgc tctaaataaa tcctctaaac cagcatattc atccaagaat
    21841 tacctttgcg tgatatttcc tcaacatcgc gacgcaaacg ttcatattgc cgcaatatta
    21901 ttttttgata tgttgaaagg cggatgcaaa tccgcacaca acatttcaaa agacaggatt
    21961 gggtaaatgg caaaggtatc gctggagaaa gacaagatta agtttctgct ggtagaaggc
    22021 gtgcaccaaa aggcgctgga aagccttcgt gcagctggtt acaccaacat cgaatttcac
    22081 aaaggcgcgc tggatgatga acaattaaaa gaatccatcc gcgatgccca cttcatcggc
    22141 ctgcgatccc gtacccatct gactgaagac gtgatcaacg ccgcagaaaa actggtcgct
    22201 attggctgtt tctgtatcgg aacaaaccag gttgatctgg atgcggcggc aaagcgcggg
    22261 atcccggtat ttaacgcacc gttctcaaat acgcgctctg ttgcggagct ggtgattggc
    22321 gaactgctgc tgctattgcg cggcgtgccg gaagccaatg ctaaagcgca ccgtggcgtg
    22381 tggaacaaac tggcggcggg ttcttttgaa gcgcgcggca aaaagctggg tatcatcggc
    22441 tacggtcata ttggtacgca attgggcatt ctggctgaat cgctgggaat gtatgtttac
    22501 ttttatgata ttgaaaacaa actgccgctg ggcaacgcca ctcaggtaca gcatctttct
    22561 gacctgctga atatgagcga tgtggtgagt ctgcatgtac cagagaatcc gtccaccaaa
    22621 aatatgatgg gcgcgaaaga gatttcgcta atgaagcccg gctcgctgct gattaatgcc
    22681 tcgcgcggta ctgtggtgga tattccggcg ctgtgtgatg cgctggcgag caaacatctg
    22741 gcgggggcgg caatcgacgt attcccgacg gaaccggcga ccaatagcga tccatttacc
    22801 tctccactgt gtgaattcga caacgtcctt ctgacgccac acattggcgg ttcgactcag
    22861 gaagcgcagg agaatatcgg cttggaagtt gcgggtaaat tgatcaagta ttctgacaat
    22921 ggctcaacgc tctctgcggt gaacttcccg gaagtctcgc tgccactgca tggtggtcgt
    22981 cgtctgatgc acatccacga aaaccgtccg ggcgtgctaa ctgcgctgaa caaaatcttc
    23041 gccgagcagg gcgtcaacat cgccgcgcaa tatctacaaa cttccgccca gatgggttat
    23101 gtagttattg atattgaagc tgacgaagac gttgccgaaa aagcgctgca ggcaatgaaa
    23161 gctattccgg gtaccattcg cgcccgtctg ctgtactaat tccccttctc tgaaaattaa
    23221 cgggcaggtc actgacttgc ccgttttttt atcccttctc cacaccgttg ccgcgttgcg
    23281 cattctactt tgcgagtctg ctccagaaaa taaatcaaag tcagatcaat gcgttgtgtt
    23341 tatttaatgg ggatttacga tggcgggaca gcatggggct gtaacggtaa agccctcacc
    23401 gaagcgaggg cttaaaggag aagggttatg atgcgacttg tcatcatact gattgtactg
    23461 ttactgataa gtttcggagc ttattaacag tcagtctcaa gggaggagtc atcctccctt
    23521 acccttactc actaaattag gtcaaagaat caacgatgtc aatcagggcg atgcggggtg
    23581 catcgccctg aaccgaactt tttgatagcc acttcatcgc ccacgtttac tgccgtaata
    23641 gatccttttt cccaaaaatt atctctgttt tgcatggggt ttttcgagca cgctcgaaga
    23701 aaagatttaa aggtagatca atgcgttgtg gttatttaaa gagagcttac gatagctggg
    23761 cagcatgggg ctgtaacggt aaagccctca ccgaagcgag ggcttgaagg agaagggtta
    23821 tgatgcgact tgccatcata ctgattgtac tgttactcat aagtttccca gcttattaac
    23881 agtcagtcac aggggaggag caatcctccc ttacccttac tcactaaatt aggttaaaga
    23941 atcaacgatg tcaatcaggg cgatgcaggt gtatcgccct taccactccc agactttcga
    24001 cggtgtaacc accgcaggaa gagggatatc ccactcttca acggggagtt tttccaccaa
    24061 ctgacaatca tgcgcataac ccaccggttg cgttttatag tgctgccagt tttgtaaggt
    24121 ccgatcataa aaaccaccgc ccattcccag gcgctgaccg tactcatcaa aggcgaccag
    24181 cggtgtgatc agcacgtcta atcgggaaag gggtagcacg tcacgcacat ccagttttgg
    24241 ctcatggatc ttcaacctgt tcatcaccag ttcgctttgc ggatggtaat tcaggaacag
    24301 caaattaccg gcactaaagg gatgcaaaac tggaagatat acgcgcttac cggcgcgcca
    24361 gagttgttct attagcggct gggtgtcgag ttcaccatca aaagagagga atacagcgac
    24421 cgtatgcgcc atcaccaccg ggggataagt catcatccgg gtagcggctt gttgacccat
    24481 ttccagctgt tgttccggcg ttaacgcacg acgacgttgc cgaatcattt tgcggatttc
    24541 ttgtcgggat aatgtcagtg ggagctctgg tagttgcgtc atggctggtg ccagataaga
    24601 agggaatctc cgagatgccg ccgcaggctg taacccttga acccttggtt caaggtgaat
    24661 gtgtcgtcgc agttttaagg cttctcggac gaaccgagca tgctcaccaa ccgcggagcg
    24721 ccacattctt gtggtatgaa atatcggctc aggggactgg cccgcttgcg aacatctcag
    24781 agaaattttg tcttcacggt tactctacca cagtaaaccg aaaagtgtca ttcaaagttt
    24841 tggttagttt tttcggtgat gcgaccttgt tcaagtaacg cttgttctat ggtctgctgc
    24901 agcatccgaa tacgctgttc catacttgcc gcgtagtcac gagtctttgc tttttcttgc
    24961 gctaactcat agctgatatt caatgcggca atgaagacca actgttcagt atttgtgact
    25021 ctagtgcgtt ctttcagatc ttgcaaccgt tggttcagat cgtccgctgc ctgattcaac
    25081 gcatcccttt ggtcaggcgg gcagttcaca cgcagtgaac ggccaaaaat ttggatatcg
    25141 acgggttgtg cagacatgcc accttcctgc tgattgactg cgctgccttc gtctttagac
    25201 ccatggtctg cgaaggggcg acactatagc taccctgatg agaagagaca agcccttttc
    25261 tggtccacca gggcccaaag tggtagcata tcatgaatat tcctcccttt gacgacgaat
    25321 gcttatgtct atacagaacg aaatgcctgg ttacaacgaa atgaaccagt atctgaacca
    25381 acaagggacg ggtctgaccc ctgccgagat gcatggttta atcagtggga tgatatgtgg
    25441 cggtaacgat gacagctcat ggctgccgct acttcacgac ctgacgaacg aaggcatggc
    25501 tttcggtcat gagctggcac aggcactgcg taaaatgcac tctgccacca gcgatgccct
    25561 gcaggatgac ggcttccttt ttcagcttta tctgcctgat ggcgatgatg tcagcgtttt
    25621 cgatcgggct gatgcactgg caggttgggt caatcacttc ctgcttggtc ttggcgttac
    25681 gcaaccgaag ctggacaaag tgaccggcga aaccggtgaa gccatcgacg atctgcgtaa
    25741 catcgcgcag ctgggctacg acgaagacga agatcaggaa gagcttgaaa tgtcgcttga
    25801 agagatcatc gaatacgttc gtgttgccgc gctgttatgc cacgacacct ttactcatcc
    25861 gcaaccgacc gcgccagaag tacaaaaacc gactctacac taaaaacaaa aaacgtaagg
    25921 agagtgttat gagtgagata tcccggcaag agtttcagcg tcgccgtcag gccctggtgg
    25981 agcaaatgca acccggcagc gccgcgctga tttttgctgc accagaagta acacgtagcg
    26041 ccgacagcga atacccctat cgtcagaaca gtgacttctg gtacttcacc ggctttaacg
    26101 aaccggaagc ggtgctggtg ctgattaaaa gcgatgacac tcataaccac agcgttctgt
    26161 ttaaccgcgt tcgcgacctg acggcggaga tctggtttgg ccgtcgctta ggccaggatg
    26221 ccgcgccaga gaaactgggc gttgaccgcg cactggcatt cagcgaaatc aatcagcaac
    26281 tttatcaact acttaacggc ctggatgtgg tttaccatgc ccagggcgaa tatgcatatg
    26341 ctgatgaaat cgtgaacagt gcgctggaaa aactgcgtaa aggttcgcgg caaaatctca
    26401 ccgcaccggc aacgatgatc gactggcgtc ctgttgttca tgaaatgcgc ctgttcaaat
    26461 cgccagaaga gattgccgta ctccgccgcg cgggagaaat caccgccatg gcacatacac
    26521 gggcgatgga aaaatgccgt ccgggaatgt tcgagtacca tctggaaggc gaaattcacc
    26581 acgaatttaa ccgccacggt gcgcgctatc cttcctataa caccattgta ggcagcggtg
    26641 aaaacggctg cattctgcac tacaccgaaa acgagagtga actgcgcgac ggtgacctgg
    26701 tgttgattga cgcgggttgt gaatacaaag gttacgcggg cgatattacc cgcaccttcc
    26761 cggtcaacgg caaattcacc caggcccagc gtgaaatcta cgacattgtg ctggagtctc
    26821 tcgaaaccag cctgcgcctg tatcgtccgg gaacttccat tctggaagtc actggtgaag
    26881 tggtgcgcat catggttagc ggcctggtaa aactcggcat cctgaaaggt aatgttgatg
    26941 aactgatcgc tcagaacgcc catcgtcctt tctttatgca tggccttagc cactggttag
    27001 gactggatgt ccatgacgtt ggcgtttatg gtcaggatcg ctcgcgcatt ctggaaccgg
    27061 ggatggtact gaccgtagag cctggtctgt atatcgcgcc ggatgcggat gtgccagaac
    27121 aatatcgcgg tatcggcatt cgcattgaag acgacattgt gattaccgaa accggcaacg
    27181 aaaacctcac cgccagcgtg gtgaaaaagc cggaagaaat cgaagcgttg atggctgctg
    27241 cgagaaagca atgagcgtaa tcatcgtcgg tggcggcatg gcgggcgcga cgctggcgct
    27301 ggctatttcc cggttaagtc acggggcgct gccggtacat ttgattgaag cgactgcgcc
    27361 agagtcacat gctcatccgg gctttgatgg acgagcgata gcgctggcgg cgggtacctg
    27421 tcagcaactg gcgcgcatcg gcgtctggca atctctggct gattgcgcaa ccgccatcac
    27481 taccgtgcat gtcagcgatc gtggtcatgc tggattcgtc accctcgccg cagaagatta
    27541 ccaactggcg gcgctggggc aggttgtcga attgcataat gtcgggcaac ggctgtttgc
    27601 attgctgcga aaagcacctg gcgtaatgct gcattgcccc gatcgcgtag ctaacgttgc
    27661 ccgtactcag agtcatgttg aagtgacgct ggagagcggc gagatgctga cgggccgcgt
    27721 gctgatagcc gctgatggca cccattcagc gttagccacc gcgtgcggcg ttgattggca
    27781 gcaggagcct tacgaacaac tggcagtgat tgccaacgtt gctacttctg ttgcgcacga
    27841 aggccgcgct tttgaacgct tcacgcaaca tggcccgctg gcgatgttgc cgatgtctga
    27901 cggacgctgt tcgctggtct ggtgtcatcc actggaacgg cgcgaagagg tgctgtcgtg
    27961 gagcgacgag aagttttgcc gtgaactcca gtctgccttt ggctggcgac tggggcaaat
    28021 tacccacgcc ggtaaacgca gtgcttatcc gctggcatta acccgcgccg ccagaccgat
    28081 tacccatcgc actgtgctgg tgggcaatgc ggcgcaaact ctgcacccga ttgccgggca
    28141 agggtttaac ctcggtatgc gagatgtcat gagccttgcg gaaaccctga ctcaggcgca
    28201 tgagcgcgga gaagacatcg gtgattacgg catattgtgc cgctatcagc agcgtcgaca
    28261 gagcgatcgc gaagcaacca tcggcgtcac ggacagcctg gtacatcttt ttgccaaccg
    28321 ttgggcaccg ctggttgtcg ggcgcaacat cggactgatg acgatggaat tattcacccc
    28381 ggcacgcgat gtgctggcgc agcgcaccct cggttgggtg gcgcgttgaa ggcggtttta
    28441 aggaataaac aatgcaaagt gttgatgtag ccattgttgg cggcggcatg gtggggctgg
    28501 cggttgcctg cggcttacag gggagcggct tacgcgttgc cgtactggag cagcgcgtac
    28561 cggaacctct ggcggtagat gcactaccac aactgcgcgt ttcggctatc aatgccgcca
    28621 gcgaaaaatt actcacccgt cttggcgtct ggcaggacat tctcgctcgt agggccagtt
    28681 gttatcacgg catggaagtg tgggacaaag acagttttgg tcacatctcg tttgacgatc
    28741 aaagcatggg ctatagccat cttgggcaca tcgttgaaaa ctcagtgatt cactacgcgc
    28801 tgtggaacaa agcgcagcag tcgtcagata ttactctgtt ggcccccaca gaattacagc
    28861 aggtcgcctg gggagaaaat gaaaccttcc tgacgctgaa agatggcagc atgttaacgg
    28921 cgcgtctggt gattggcgcg gacggtgcta attcctggtt gcgcaacaaa gcagatattc
    28981 cgctgacttt ctgggattat cagcatcacg cgctggtagc gaccattcgc acggaagaac
    29041 cgcatgatgc ggtggcgcgg caggttttcc atggcgaagg cattctggcc tttttaccgc
    29101 ttagcgatcc gcatctttgc tcgattgtct ggtcactgtc gccagaggaa gcgcagcgga
    29161 tgcagcaggc aagtgaagac gaatttaatc gcgcgttaaa tatcgctttt gataatcgcc
    29221 ttggcttatg caaggttgag agcgcgcgtc aggtgttccc gctgacgggg cgttatgcgc
    29281 gccagtttgc cgcacaccgt ctggcgctgg tgggcgacgc cgcgcatacc attcacccac
    29341 tggcggggca gggcgttaat ctcggattta tggacgccgc agagctgatt gctgaactga
    29401 aacggttgca tcgtcagggt aaagacatcg ggcagtacat ttacctgcgc cgctatgaac
    29461 gtagccgcaa gcacagcgcg gcgctgatgc tggctggtat gcagggtttt cgcgatctct
    29521 tttccggcgc taatccggcg aaaaaactgc tgcgtgatat tggtttgaaa ctggccgaca
    29581 cgcttcctgg cgttaagccg caacttatcc gccaggcaat gggattaaac gatttgcctg
    29641 aatggctgcg ttaaaaattt ctcctctgtt gtttatttga tacccatcac actttcatct
    29701 cccggttttt tcgccgggag attttcctca tttgaaataa actaatttca cctccgtttt
    29761 cgcattatat tttctaatac catgattttt tgatttagtg ttttttgaca tttttttagc
    29821 tcttaatatt gtcttattca aattgacttt ctcatcacat catctttgta tagaaattgg
    29881 tgtatttttt ggttttttat tctgtcgcga tttttgcatt ttttaaccat aagctaatgt
    29941 gatgatcaat tttaccttat ggttaacagt ctgtttcggt ggtaagttca ggcaaaagag
    30001 aacgattgcg ttggggaccg ggagtggctc cgatgctggg ttttgtggtg ataatttcac
    30061 catgaaaaag ttgtcagccc cgcttattca atgaggacaa gatggcacaa cagactcctt
    30121 tgtacgaaca acacacgctt tgcggcgctc gcatggtgga tttccacggc tggatgatgc
    30181 cgctgcatta cggttcgcaa atcgacgaac atcatgcggt acgtaccgat gccggaatgt
    30241 ttgatgtgtc gcatatgacc atcgtcgatc tccgcggcag ccgcacccgg gagtttctgc
    30301 gttatctgct ggcgaacgat gtggcgaagc tcaccaaaag aggcaaagcc ctttactcgg
    30361 ggatgttgaa tgcctctggc ggtgtgatag atgatctcat cgtctactac tttactgaag
    30421 atttcttccg cctcgttgtt aactccgcca cccgcgaaaa agacctctcc tggattaccc
    30481 aacacgctga acctttcggc atcgaaatta ccgttcgtga tgacctttcc atgattgccg
    30541 tgcaagggcc gaatgctcag gcaaaagctg ccacactgtt taatgatgct cagcgtcagg
    30601 cggtggaagg gatgaaaccg ttctttggcg tgcaggtggg cgatctgttt attgccacca
    30661 ctggttatac cggtgaagcg ggctatgaaa ttgcgctgcc caatgaaaaa gcggccgatt
    30721 tctggcgtgc gctggtggaa gcgggcgtta agccctgcgg tctgggggcg cgtgacacgc
    30781 tgcgtctgga agcgggcatg aatctttatg gtcaggagat ggacgaaact atttctcctt
    30841 tagccgccaa catgggctgg actatcgcct gggaaccgac agatcgtgac tttatcggtc
    30901 gtgaagccct ggaagtgcag cgtgagcatg gtacagaaaa actggttggt ctggtgatga
    30961 ccgaaaaagg cgtgctgcgt aatgaactgc cggtacgctt taccgatgcg cagggcaacc
    31021 agcatgaagg cattatcacc agcggtactt tctccccgac gctgggttac agcattgcgc
    31081 tggcgcgcgt gccggaaggt attggcgaaa cggcgattgt gcaaattcgc aaccgtgaaa
    31141 tgccggttaa agtgacaaaa cctgtttttg tgcgtaacgg caaagccgtc gcgtgattta
    31201 cttttttgga gattgattga tgagcaacgt accagcagaa ctgaaataca gcaaagaaca
    31261 cgaatggctg cgtaaagaag ccgacggcac ttacaccgtt ggtattaccg aacatgctca
    31321 ggagctgtta ggcgatatgg tgtttgttga cctgccggaa gtgggcgcaa cggttagcgc
    31381 gggcgatgac tgcgcggttg ccgaatcggt aaaagcggcg tcagacattt atgcgccagt
    31441 aagcggtgaa atcgtggcgg taaacgacgc actgagcgat tccccggaac tggtgaacag
    31501 cgaaccgtat gcaggcggct ggatctttaa aatcaaagcc agcgatgaaa gcgaactgga
    31561 atcactgctg gatgcgaccg catacgaagc attgttagaa gatgagtaac ggctttattc
    31621 ctcttctgcg ggagaggatc agggtgagga aaatttatgc ctcaccctca ctctcttcgt
    31681 aaggagagag gttcacaatt cactgcacgt ttcaggaacc atcgctcatg acacagacgt
    31741 taagccagct tgaaaacagc ggcgctttta ttgaacgcca tatcggaccg gacgccgcgc
    31801 aacagcaaga aatgctgaat gccgttggtg cacaatcgtt aaacgcgctg accggccaga
    31861 ttgtgccgaa agatattcag cttgcgacac caccgcaggt tggcgcaccg gcgaccgaat
    31921 acgccgcact ggcagaactc aaggctattg ccagtcgcaa taaacgcttc acgtcttaca
    31981 tcggcatggg ttacaccgcc gtgcagctac cgccggttat cctgcgtaac atgctggaaa
    32041 atccgggctg gtataccgcg tacactccgt atcagcctga agtctcccag ggccgccttg
    32101 aagcactgct caacttccag caggtaacgc tggatttgac tggactggat atggcctctg
    32161 cttctcttct ggacgaggcc accgctgccg ccgaagcaat ggcgatggcg aaacgcgtca
    32221 gcaaactgaa aaatgccaac cgcttcttcg tggcttccaa tgtgcatccg caaacgctgg
    32281 atgtggtccg tactcgtgct gaaacctttg gttttgaagt gattgtcgat gacgcgcaaa
    32341 aagtgctcga ccatcaggac gtcttcggcg tgctgttaca gcaggtaggc actaccggtg
    32401 aaattcacga ctacactgcg cttattagcg aactgaaatc acgcaaaatt gtggtcagcg
    32461 ttgccgccga tattatggcg ctggtgctgt taactgcgcc gggtaaacag ggcgcggata
    32521 ttgtttttgg ttcggcgcaa cgcttcggcg tgccgatggg ctacggtggc ccacacgcgg
    32581 cattctttgc ggcgaaagat gaatacaaac gctcaatgcc gggccgtatt atcggtgtat
    32641 cgaaagatgc agctggcaat accgcgctgc gcatggcgat gcagactcgc gagcaacata
    32701 ttcgtcgtga gaaagcgaac tccaacattt gtacttccca ggtactgctg gcaaacatcg
    32761 ccagcctgta tgccgtttat cacggcccgg ttggcctgaa acgtatcgct aaccgcattc
    32821 accgtctgac cgatattctg gcggcgggcc tgcaacaaaa aggtctgaaa ctgcgccatg
    32881 cgcactattt cgacaccttg tgtgtggaag tggccgacaa agcgggcgta ctgacgcgtg
    32941 ccgaagcggc tgaaatcaac ctgcgtagcg atatcctgaa cgccgtgggg atcacccttg
    33001 atgaaaccac cacgcgtgaa aacgtaatgc agcttttcag tgtgctgctg ggcgataacc
    33061 acgggctgga gatcgacacg ctggacaaag acgtggctca cgacagccgc tctatccagc
    33121 ctgcgatgct gcgcgacgac gaaatcctca cccatccggt gtttaatcgc tatcacagcg
    33181 aaaccgaaat gatgcgctat atgcactcgc tggagcgtaa agatctggcg ctgaatcagg
    33241 cgatgatccc gctgggttcc tgcaccatga aactgaacgc cgccgccgag atgatcccaa
    33301 tcacttggac ggaatttgcc gaactgcacc cgttttgccc gccggaacag gctgaaggtt
    33361 atcagcagat gattgcgcag ctggctgact ggctggtgaa actgaccggt tacgacgccg
    33421 tttgtatgca gccgaactcc ggcgcacagg gcgaatacgc gggcctgctg gcgattcgtc
    33481 attatcatga aagccgcaac gaagggcatc gcgatatctg cctgatcccg gcttctgcgc
    33541 acggaactaa ccccgcttct gcacatatgg caggaatgca ggtggtggtt gtggcgtgtg
    33601 ataaaaacgg caacatcgat ctgactgatc tgcgcgcgaa agcggaacag gcgggcgata
    33661 acctctcctg tatcatggtg acttatcctt ctacccacgg cgtgtatgaa gaaacgatcc
    33721 gtgaagtgtg tgaagtcgtg catcagttcg gcggtcaggt ttaccttgat ggcgcgaaca
    33781 tgaacgccca ggttggcatc acctcgccgg gctttattgg cgcggacgtt tcgcacctca
    33841 acctgcataa aactttctgc attccgcacg gcggtggtgg tccgggtatg ggaccgatcg
    33901 gtgtgaaagc gcatctggca ccgtttgtac cgggtcatag cgtggtgcaa atcgaaggca
    33961 tgttaacccg tcagggcgca gtttctgcgg caccgttcgg tagcgcctct atcctgccaa
    34021 tcagctggat gtacatccgc atgatgggcg cagaagggct gaaaaaagca agccaggtgg
    34081 caatcctcaa cgccaactat attgccagcc gcctgcagga tgccttcccg gtgctgtata
    34141 ccggtcgcga cggtcgcgtg gcgcacgaat gtattctcga tattcgcccg ctgaaagaag
    34201 aaaccggcat cagcgagctg gatattgcca agcgcctgat cgactacggt ttccacgcgc
    34261 cgacgatgtc gttcccggtg gcgggtacgc tgatggttga accgactgaa tctgaaagca
    34321 aagtggaact ggatcgcttt atcgacgcga tgctggctat ccgcgcagaa attgaccagg
    34381 tgaaagccgg tgtctggccg ctggaagata acccgctggt gaacgcgccg cacattcaga
    34441 gcgaactggt cgccgagtgg gcgcatccgt acagccgtga agttgcggta ttcccggcag
    34501 gcgtggcaga caaatactgg ccgacggtga aacgtctgga tgatgtttac ggcgaccgta
    34561 acctgttctg ctcctgcgta ccgattagcg aataccagta attcactgat tcgagtattt
    34621 tctaaaggcg cttcggcgcc tttttagtca gatgacaaag tacaaaagtg ctcagacagt
    34681 cccctcgccc ctttggggag agggttaggg tgaggggaac aggccggcac tggcgcgaat
    34741 atttaccctc accccgaccc tctccctgaa agggcgaggg ggaaaagcgt gccaacattg
    34801 aagattgagc cagtttgtta gcaatctcaa agatacgtca acgaattaat ttttctcgga
    34861 aaaacaaatg gctatagcac ttgtgaccgg tggcagtcgc ggcatcgggc gggcaactgt
    34921 attactgttg gcgcaagaag ggtatacggt ggcggttaat tatcagcaaa acctccacgc
    34981 ggcgcaggaa gtgatgaact taataatgca agccggtggc aaagcattcg tgctccaggc
    35041 ggatatcagc gacgaaaacc aggtcgttgc gatgtttaca gcaatcgatc agcacgatga
    35101 accgctagca gcgctggtca ataacgccgg gatcttgttt acccagtgca ccgttgaaaa
    35161 ccttaccgca gagcgaatca accgagtact ttccaccaac gtgacgggat attttctctg
    35221 ctgccgcgag gcggtaaaac gcatggcgct taaaaatggt ggcagtggcg gcgctatcgt
    35281 caatgtctct tcggtggcct cacggttggg ttcgccaggg gaatatgttg attacgcggc
    35341 atcgaaaggg gcgattgatt cgttaaccac cggactatcg ctggaagtcg ccgcgcaggg
    35401 gatccgcgtt aactgcgtgc ggccagggtt tatttatacc gaaatgcacg ccagcgcggc
    35461 gagcctggac gcgtcgatcg cgttaagtcg aacatcccca tgcagcgtgg tggacaggca
    35521 gaagaggtcg cgcaggccat tgtctggcta ctaagtgata aagcctctta cgtcacggga
    35581 agttttatcg atttggcggg cgggaaataa aacagggaag ttgtctgacc ggatgcaaca
    35641 agtattgcat ccggtacttc atcgacttaa agcttctcgc cgttgctggc aatcacctct
    35701 ttgtaccagt taaagctctt cttacgtgaa cgcgacatat cgccagtacc gtcgtcatgt
    35761 ttattcacat agataaagcc gtagcgtttg ctgtactgcc cggtagtgaa tgacacgcag
    35821 tcgatgcagc cccacggtgt gtagcccatc agatccacac catcgtaagt cactgctttt
    35881 ttcatctctt caatatgggc gcgcaggtag tcaatgcggt agtcgtcgtt gatgctgcca
    35941 tcttcttcca ctttgtcgta agcgccaaaa ccgttttcga caataaacag cggcctctga
    36001 taacgctcat acagttcgca aagggcatag cgcagaccta ctggatcaat ctgccagccc
    36061 cagtcagacg ctttaacata cgggttcggt acgctgcctt caaaaccaga gatcgcatcg
    36121 ccggtgccgc cttcggcctt cactgcgttg gtcatgtaat agctgaaacc aagataatcg
    36181 caggtgcctt cgcgcagtac atcgagatcg ccgtcttcca ttttgatatt aaatccgcga
    36241 cgttcccact cgttcaatac ataggacggg taatagccgc atagctgcac atcggtaaag
    36301 acgtagcgtt cgcgcatcga ctcctgagcg aacatcacat cgtccgggtt acatgagtag
    36361 ggatagagcg gcaccatcgc cagcatacag ccgactttca tctccgggtt aatgcgacgc
    36421 gcggctttca ctgccagggc gctggcgaca aactggtgat gcagcacctg gtacatcgtc
    36481 tcttccgggt tgtcatgctc ggtatacacc acgccggagc agcagtaacc aaacagcggc
    36541 gcacgccagt tacgctggtt gtttatttcg ttgaaggtca tccagtattt gactttatgc
    36601 ttgtagcgtt cgaataccac ttccgcgaaa cggacaaaga aatcaacgaa tttacggttg
    36661 gtccagctac cgtattgctg caccagatgc agcggcattt caaagtggga gagggttatc
    36721 accggttcga tgttgtattt cagcagttca tcgaacagag agtcgtaaaa cttcagccct
    36781 tcttcgtttg gctgagcttc atcacctttt ggaaaaatac gcgtccaggc gatggaagtc
    36841 cgaaaacatt tgaagcccat ttcggcaaat agcttgatgt cttccttgta gtgaccataa
    36901 aaatcaacgg cttcatggtt tggatagtat tttcctggca agacttcttt ggtgatttcg
    36961 cgcggcacgc cgtgtgcgcc accggtcaga acgtcacaaa tgctcggtcc tttgccgcct
    37021 ttgttccagc cgccttcgac ctgatgagcg gcaacggcgc caccccataa gaaatctttc
    37081 ggtaaggtga gttttttcac tatcataggc tccagatcag tcaattctta caggaaatag
    37141 taccatgcag ccaaacgaca tcactttttt caacgtttcc aggatgacat tctggctggg
    37201 cgtaaaacca tcaccatccg cgacgagtct gaatcgcact tcaaaacggg tgatgtgctt
    37261 cgtgtcggac gttttgaaga tgacggttat ttttgcacga ttgaagtcac cgcaacctca
    37321 accgtaacgc tggatacact gacggaaaaa catgcagagc aggaaaatat gaccctgcct
    37381 gaactgaaaa aggtcattgc tgacatctat cccggtcaga cacaatttta tatgattgaa
    37441 tttaaatgtc tttaaattga actcacgcct gttagttgaa atttcgcatt taatttcatg
    37501 atttttagga gattagtctc aaatcagaat ttattttagc taacaggtgt tcactggaac
    37561 tattctcagt tacgctggag aggtatacat gtgcgtgtac gtttctctgg agtaagttat
    37621 ggttcagaag cccctcatta agcagggata ttcgctggca gaggaaattg ccaacagcgt
    37681 cagtcacggc attgggctgg tgtttggtat cgttgggctg gtgttgctac tggttcaggc
    37741 ggtggatctc aatgccagcg ccacagcgat aaccagttac agcctctatg gcggcagtat
    37801 gatcctgctg ttcctcgctt cgacgctcta tcacgccatt cctcatcaac gggcaaaaat
    37861 gtggctgaag aaatttgacc attgcgctat ttacctgttg attgccggaa cctacacgcc
    37921 gtttttgctg gtggggctgg attctccgtt agcgcgcggg ttgatgattg ttatctggag
    37981 cctagcattg ctggggattc tgttcaaact gaccatcgcg caccgattca aaattttatc
    38041 tctggtgact tatctggcga tgggctggct gtcgctggtg gtaatttatg aaatggcagt
    38101 taagctcgcg gcgggcagcg ttaccttact ggcggtaggc ggcgtggttt attcgctcgg
    38161 ggtgattttc tacgtctgca aacgcattcc atacaaccat gccatctggc acggcttcgt
    38221 gctcggcggt agtgtgtgcc actttctggc gatctatttg tatattgggc aggcgtaatg
    38281 ggtatgcctt tgtaggcctg ataagacgcg tcagcgtcgc atcaggcatc ggagcgcaac
    38341 cgccggatgc ggcgtgaacg ccttatccga tctacgggcc tcgtccctgt tgtgataaag
    38401 gcatcaaatc ccagacatga taagacgcgt cagcgtcgca tcaggcgata aatgcctgat
    38461 gcgatacaga gatttactct tcgagcgaat acggcagcgg ctcgatatgc agtgtattcg
    38521 catcgtcgcg tacgcggaag atgctgtccg gttccatatc gttattcatg acgacctgta
    38581 ccacgacctg accatcttcc agttttaccg cagccagcac cgtaccggta cgacgccagt
    38641 tctcgcccat tttcagctct aagtcttcac cagcttccgg cagtcggctg acactacctg
    38701 ccagcaacca gagcgcgcgt ttgttggcac cacggaattt tgctcgcgcc accatctctt
    38761 gtccggtata acagcctttc ttaaagctga taccgcccag cgcctggaga ttggtcgcct
    38821 gtgggataaa ctgcccgctg ttggcggcat caatcaccgg gaaaccagct tcaatgttta
    38881 atgccagcca ctgttggcta ttgttcagtt ccgcttcacc gcgcagttta tcggtcagca
    38941 tattagcagt agcttcatcg gttacgatca ggaaacgttc tgccgggtgt ttaaaccata
    39001 gcaaagtggt cgcgccttct ttgactacct gtttttcttt cgaaggcagt tcgctaaaga
    39061 gatttgccag cgcggcgcgc gcctgaaaac cggcaacacc aagcagcaca cgctcgtcgt
    39121 ctggcgcgat ggtcacttta gagaataccg catatttttt cagttcagtc agctgcggtt
    39181 cacgcacgct gcgccgttca atccatgcaa agccatcgcc gtcgcggaac agacgtaaat
    39241 tgctccacat tttacctttg gcgtcgcaat gggcggcgag caggtgctga tcttctgcca
    39301 tctggctgac atctgctgtc acctgaccct gcatatattt ttcgctgtcc gcgccagtaa
    39361 tggtggcaag cgcccagtca tcaagcgtca tcagcgtcag cggtaaacgg gcagaagccg
    39421 taggctgacg tggaggaaaa ggtgtaaaag ccataataga gtcctgagtt gcttaacggc
    39481 gtattaatga ctaatggtaa aagagctatg aggcaatgca agtgctttca ctacgcgatc
    39541 tgtgccttct gcgaggcgac tgccaaaaaa atgaagccct tgaattgttg tgaattaatt
    39601 tcgcgatcgc gatggtgttt actgatattc ccgtaaaaga agtcgaaaaa cacgttacaa
    39661 tagccgggta ctggattttc gtgagacagg aagaagatgg acattaacaa caaagcccga
    39721 attcattggg catgccgccg tggtatgcgc gaactcgata tttcaatcat gccgtttttc
    39781 gaacatgagt acgacagctt aagcgatgac gaaaaacgca tctttattcg tctgctggaa
    39841 tgtgacgatc cggacctgtt taactggctg atgaatcacg gtaaaccggc cgatgcagaa
    39901 ctggaaataa tggtccgact catccagaca cggaaccggg aacgtggtcc tgtggcaatc
    39961 tgatttgcgc gtctcctggc gcgcacagtg gctttccttg ctgattcatg ggctggttgc
    40021 cgctgttatt ttactcatgc cctggccgct cagttacacc ccgttatgga tggtgttact
    40081 ttcgctggtg gtatttgatt gcgttcgcag ccagcggtgt attaatgctc gccaggggga
    40141 aattcgcttg ttgatggacg ggcgtttgcg ttggcaaggg caggagtgga gcatcgtcaa
    40201 agcaccgtgg atgattaaga gcggcatgat gctgcgttta cgttctgatg gcggtaaacg
    40261 gcaacattta tggctggcag ccgacagcat ggacgaagcc gaatggcggg atttacggcg
    40321 gattttgttg caacaagaga cgcaaagata aagatgacgg caaagcatat gcgctaagcc
    40381 gtcagtgaga tcaggcgtaa tgctctgcca tttcgttgag gatttgctcg caccagctct
    40441 gaatacgctc gtcgctaagg tcatactggt tagtttcatc cagcgccaga cccacgaaca
    40501 gttgcccgtc agcaatcacc ggtttcgggc tggtaaattc atatccttcc gttggccagt
    40561 agccgacgaa cttcacgcct ttggtcgaga gtttgtcatg cagcataccg agcgcatcga
    40621 ggaaccactc gccgtatccc agttgatcgc caagcccata cagcgcaata attttacctt
    40681 caaggttcag gtcgtcgagc tgatcccaga cggcttccca gtcttcctgg atttcaccaa
    40741 aatcccaggt cgggataccc agaatgagca catcgtactg ctccattaat ttcggggagt
    40801 cgtccttgag gttatgtaag gtcaccagtt ctgggccgat aatatcgcgg attttttctg
    40861 ccgccatttc ggtgtaacag gtgctggaac cgtaaaaaag acccatattc ataacgtaag
    40921 cttctcgatt ctgctgtggc ttttgcagct agtgtatcag aatcgcttta gtcacggcac
    40981 aataaggcat aatggatcaa atgagtgacc ataaggggcg caagtgaaac aggagctggc
    41041 acgcatcgag cagtttcttg atgctctgtg gctggaaaaa aatctggctg aaaatacgtt
    41101 gaacgcttac cgtcgcgatc tgtcaatgat ggtggagtgg ttgcatcacc gcgggttgac
    41161 gctggcgacg gcgcaaagtg acgatttgca ggcattactg gcagaacggc tggagggcgg
    41221 gtataaagcc accagctcag cgcgtttgct gagtgcagtg cgccgattgt tccagtatct
    41281 ttatcgcgaa aagtttcgtg aagacgatcc cagtgcgcat ctcgcttcac cgaaattgcc
    41341 ccagcgtttg ccaaaagatt taagcgaagc gcaggtcgaa cgtttattac aggcgccatt
    41401 aattgatcag ccactggagc tacgcgataa agccatgctt gaagtgttgt atgctaccgg
    41461 gctgcgtgtc tctgaactgg tcgggctgac aatgagtgat atcagcctgc gtcagggcgt
    41521 agtgcgggtc attggtaaag gcaacaaaga gcgcctggtg ccgttagggg aagaggcggt
    41581 ttactggctg gaaacctatc tggaacatgg gcgtccgtgg ctgctgaatg gtgtgtcaat
    41641 tgacgtgttg tttcccagcc agcgtgcgca gcagatgacg cgacagacct tctggcaccg
    41701 cattaaacat tatgccgtgc tggcgggtat cgacagcgaa aagctgtcac cgcatgtgtt
    41761 gcgtcacgct tttgccactc atttattaaa tcatggtgcg gatttacgcg tggtgcagat
    41821 gctactgggc cacagcgatc tttccaccac gcaaatttat acgcatgtcg ctaccgagcg
    41881 tctgcggcaa cttcatcaac agcatcaccc gcgggcgtga tgtctgaaaa gaacgggaag
    41941 atttatgaag aaaggtttta tgttgtttac tttgttagcg gcgttttcag gctttgctca
    42001 ggctgatgac gcggcaattc aacaaacgtt agccaaaatg ggcatcaaaa gcagcgatat
    42061 tcagcccgcg cccgtagctg gcatgaagac agttctgact aacagcggtg tgttgtacat
    42121 caccgatgat ggtaaacata tcattcaggg gccaatgtat gacgttagtg gcacggctcc
    42181 ggtcaatgtc accaataaga tgctgttaaa gcagttgaat gcgcttgaaa aagagatgat
    42241 cgtttataaa gcgccgcagg aaaaacacgt catcaccgtg tttactgata ttacctgtgg
    42301 ttactgccac aaactgcatg agcaaatggc agactataac gcgctgggga tcaccgtgcg
    42361 ttatcttgct ttcccgcgcc aggggctgga cagcgatgca gagaaagaaa tgaaagctat
    42421 ctggtgtgcg aaagataaaa acaaagcgtt tgatgatgtg atggcaggta aaagcgtcgc
    42481 accagccagc tgcgacgtgg atattgccga ccattacgca cttggcgtcc agcttggcgt
    42541 tagcggtact ccggcagttg tgctgagcaa tggcacactt gttccgggtt accagccgcc
    42601 gaaagacatg aaagaattcc tcgacgaaca ccaaaaaatg accagcggta aataattcgc
    42661 gtgaaacaac agatacaact tcgtcgccgt gaagtcgatg aaacggcaga cttgcccgct
    42721 gaattgcctc ccttgctgcg ccgtttatac gccagccggg gagtacgcag tgcgcaagaa
    42781 ctggaacgca gtgttaaagg tatgttgccc tggcagcaac taagcggtgt cgaaaaggcc
    42841 gttgagatcc tttacaacgc ttttcgcgaa ggaacgcgga ttattgtggt cggtgatttc
    42901 gacgccgacg gcgcgaccag cacggctcta agcgtgctgg cgatgcgctc gcttggttgc
    42961 agcaatatcg actacctggt accaaaccgt ttcgaagacg gttacggctt aagcccggaa
    43021 gtggtcgatc aggcccatgc ccgtggcgcg cagttaattg tcacggtgga taacggtatt
    43081 tcctcccatg cgggagttga gcacgctcgc tcgttgggca tcccggttat tgttaccgat
    43141 caccatttgc cgggcgacac attacccgca gcggaagcga tcattaaccc taacttgcgc
    43201 gactgtaatt tcccgtcgaa atcactggca ggcgtgggtg tggcgtttta tctgatgctg
    43261 gcgctgcgca cctttttgcg cgatcagggc tggtttgatg agcgcggcat cgcaattcct
    43321 aacctggcag aactgctgga tctggtagca ctggggacag tagcggacgt cgtgccgctg
    43381 gacgctaata atcgcattct gacctggcag gggatgagtc gcatccgtgc cggaaagtgc
    43441 cgtccaggga ttaaagcgct gctggaagtg gcaaatcgcg atgcacaaaa actagccgcc
    43501 agcgatttag gttttgcgct gggaccgcgt ctcaatgctg ccgggcgact ggatgatatg
    43561 tccgttggtg tggcgctgtt gctgtgcgac aacatcggcg aagcgcgcgt gctggcaaat
    43621 gaactcgatg cgctaaacca gacgcgaaaa gagatcgaac aaggaatgca ggttgaagcc
    43681 ctgaccctgt gcgagaaact ggagcgcagc cgtgacacgc tacccggcgg gctggcaatg
    43741 tatcaccccg aatggcatca gggcgttgtc ggtattctgg cttcgcgtat caaagagcgt
    43801 tttcaccgtc cggttatcgc ctttgcgcca gcaggtgacg gtacgctgaa aggttccggt
    43861 cgctccattc aggggctgca tatgcgtgat gcgctggagc gattagacac actctacccc
    43921 ggcatgatgc tcaagtttgg cggtcatgcg atggcggcgg gtttgtcgct ggaagaggat
    43981 aaattcgaac tctttcaaca acggtttggc gaactggtta ctgagtggct ggccccttcg
    44041 ctattgcaag gcgaagtggt gtcagacggt ccgttaagcc cggccgaaat gaccatggaa
    44101 gtggcgcagc tgctgcgcga tgctggcccg tgggggcaga tgttcccgga gccgctgttt
    44161 gacggtcatt tccgtctgct gcaacagcgg ctggtgggcg aacgtcattt gaaggtgatg
    44221 gtcgaaccgg tcggcggcgg tccactgctg gatggtattg cttttaatgt cgataccgct
    44281 ctctggccgg ataacggcgt gcgcgaagtg caactggctt ataagctcga tatcaacgag
    44341 tttcgcggca accgcagcct gcaaattatc atcgacaata tctggccaat ttagcgtcat
    44401 cttctctata aaaaagagcg tggattgggt acaatcccgc tcttatcacc gcattttgac
    44461 tagctcaata aaagaaatca gaccatgttt gaaattaatc cggtaaataa tcgcattcag
    44521 gacctcacgg aacgctccga cgttcttagg gggtatcttt gactacgacg ccaagaaaga
    44581 gcgtctggaa gaagtaaacg ccgagctgga acagccggat gtctggaacg aacccgaacg
    44641 cgcacaggcg ctgggtaaag agcgttcctc cctcgaagcc gttgtcgaca ccctcgacca
    44701 aatgaaacag gggctggaag atgtttctgg tctgctggaa ctggctgtag aagctgacga
    44761 cgaagaaacc tttaacgaag ccgttgctga actcgacgcc ctggaagaaa aactggcgca
    44821 gcttgagttc cgccgtatgt tctctggcga atatgacagc gccgactgtt acctcgatat
    44881 tcaggcgggg tctggcggta cggaagcaca ggactgggcg agcatgcttg agcgtatgta
    44941 tctgcgctgg gcagaatcgc gtggtttcaa aactgaaatc atcgaagagt cggaaggtga
    45001 agtggcgggt attaaatccg tgacgatcaa aatctccggc gattacgctt acggctggct
    45061 gcgtacagaa actggcgttc accgcctggt gcgtaaaagc ccgtttgact ccggcggtcg
    45121 tcgccacacg tcgttcagct ctgcgtttgt ttatccggaa gttgatgatg atattgatat
    45181 cgaaatcaac ccggcggatc tgcgcattga cgtttatcgc gcgtccggcg cgggcggtca
    45241 gcacgttaac cgtaccgaat ctgcggtgcg tattacccac atcccgaccg ggatcgtgac
    45301 ccagtgccag aacgaccgtt cccagcacaa gaacaaagat caggccatga agcagatgaa
    45361 agcgaagctt tatgaactgg agatgcagaa gaaaaatgcc gagaaacagg cgatggaaga
    45421 taacaaatct gacatcggct ggggcagcca gattcgttct tatgtccttg atgactcccg
    45481 cattaaagat ctgcgcaccg gggtagaaac ccgcaacacg caggccgtgc tggacggcag
    45541 cctggatcaa tttatcgaag caagtttgaa agcagggtta tgaggaacca acatgtctga
    45601 acaacacgca cagggcgctg acgcggtagt cgatcttaac aatgaactga aaacgcgtcg
    45661 tgagaagctg gcgaacctgc gtgagcaggg aattgccttc ccgaacgatt tccgtcgcga
    45721 tcatacctct gaccaattgc acgcagaatt cgacggtaaa gagaacgaag aactggaagc
    45781 gctgaacatc gaagtcgccg ttgctggccg catgatgacc cgccgtatta tgggtaaagc
    45841 gtctttcgtt accctgcagg atgtgggtgg ccgcattcag ctgtacgttg cccgtgacga
    45901 tctgccggaa ggcgtttaca acgagcagtt caaaaaatgg gacctcggcg acatcctcgg
    45961 cgcgaaaggt aaactgttca aaaccaaaac cggcgaactg tctatccact gtaccgagct
    46021 gcgtctgctg accaaagcac tgcgtccgct gccggataaa ttccacggct tgcaggatca
    46081 ggaagcgcgc tatcgtcagc gttatctgga tctcatctct aacgatgaat cccgtaacac
    46141 ctttaaagta cgctcgcaga tcctctctgg tattcgccag ttcatggtga accgcggctt
    46201 tatggaagtt gaaacgccga tgatgcaggt gatcccaggc ggtgccgctg cgcgtccgtt
    46261 tatcacccac cataacgcgc tggatctcga catgtacctg cgtatcgcgc cggaactgta
    46321 cctcaagcgt ctggtggtcg gtggcttcga gcgtgtattc gaaatcaacc gtaacttccg
    46381 taacgaaggt atttccgtac gtcataaccc agagttcacc atgatggaac tctacatggc
    46441 ttacgcagat tacaaagatc tgatcgagct gaccgaatcg ctgttccgta ctctggcaca
    46501 ggatattctc ggtaagacgg aagtgaccta cggcgacgtg acgctggact tcggtaaacc
    46561 gttcgaaaaa ctgaccatgc gtgaagcgat caagaaatat cgcccggaaa ccgacatggc
    46621 ggatctggat aacttcgact ctgcgaaagc gattgctgaa tctatcggta tccacgttga
    46681 gaagagctgg ggtctgggcc gtatcgttac cgagatcttc gaagaagtgg cagaagcaca
    46741 tctgattcag ccgaccttca ttactgaata tccggcagaa gtttctccgc tggcgcgtcg
    46801 taacgacatt aacccggaaa tcacagaccg ctttgagttc ttcatcggtg gtcgtgaaat
    46861 cggtaacggc tttagcgagc tgaatgacgc ggaagatcag gcgcaacgct tcctggatca
    46921 ggttgccgcg aaagacgcag gtgacgacga agcgatgttc tacgatgaag attacgttac
    46981 cgcactggaa catggcttac cgccgacagc aggtctgggg attggcatcg accgtatggt
    47041 aatgctgttc accaacagcc ataccatccg cgacgttatt ctgttcccgg cgatgcgtcc
    47101 ggtgaaataa gcattacgtt atgctcacaa ccccggcaaa tgtcggggtt ttttatttaa
    47161 gctgggtaaa tgcagataat cgtattctgg cttcgcgatt tgtcgcctgc atcaccatcc
    47221 acggactgaa cgcccacggc gtggcatcaa taccgcgtaa tacatcagct aaatcacacc
    47281 attgataatc catcacttca tcatcgttga tctgtaacgc actggtggtg cgtgcggcaa
    47341 ataccggaca cacttcattt tccacaatgc ctctcggatc ggtggcgcgg tagcgaaagt
    47401 caggatagat agattcagga ggcgtaattt ccacgccaag ctcataacgg caacggcgga
    47461 tcaccgcgtc ttcgctgctt tctcccagtt gtgggtgccc acaaaccgag ttagtccaca
    47521 cgccaggcca cgcttttttg ctcagtgcgc ggcgggtaac taataattgc cccttggcat
    47581 taaacagcca actggagaac gcgagatgta agagggtgtc tgccgtgtgt gcggcatact
    47641 tttccagcgt acccgtggga actccctgtg cattcaataa aatgacgtgt tccgtttgca
    47701 taatttctca catgtaattc tgatcactca tcattataga taattgaaga gtgaaatgat
    47761 taaccaagag agcgtttatc gacaaccgcc cgttttgagc ggatgccgac aggtaaaaac
    47821 catttaaaaa agtagcatca tatatttgaa gcgactttca atggccattg caacacagtc
    47881 agaaatgaca ggaatagaga gagtaaaaag caacaacaga ctgaatatag cgcgcattaa
    47941 aaaattcata ttattctcca tgctcatttt tcttcagctt gcgcatcaga agccacatac
    48001 gtactgtcct gacttctaaa tctttatcag agacggattc cttcaccagg tcagcatgat
    48061 gctgatagcc attaaagaag acgttcagca caacggcgct aagggttgcg agcataatgc
    48121 cgctatgtag cagcggttgc agtacggccg gtaacttaga aaagaaatcg tgagagagcg
    48181 tcggagtcat gccaacgccg agactgatcg ccacaatata gaggttgtaa cggttggtgg
    48241 tgtagttaca gcgcgacaga attcgaatcc ctgtcgccag taccatgccg aacatcacta
    48301 gaccagcgcc gcccagcaca aattgcggaa tggaggctac cagcaccgcc atttttggca
    48361 ccatgccaaa caggattaat ataattcccg aagaaataca cacccagcgg ctatgaacgc
    48421 gcatcacgct aaccaggcca acgttttgag aaaatgacgt gtgggggaag ctgttaaacg
    48481 tgccgcctat cattgtccct acgccatcga cacgcagccc gcaaataata tcgtgtgaag
    48541 agagtttacg accgactatt tcacccagtg ccaggaacat ccccatcgac tcgataaaca
    48601 cgatgat
//
LOCUS       DAEEUE010000022        48313 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.022, whole genome shotgun sequence.
ACCESSION   DAEEUE010000022 DAEEUE010000000
VERSION     DAEEUE010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48313
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.022"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            187..480
                     /locus_tag="KLK84_001318"
     CDS             187..480
                     /locus_tag="KLK84_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBE2008708.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(521..1711)
                     /gene="nepI"
                     /locus_tag="KLK84_001319"
     CDS             complement(521..1711)
                     /gene="nepI"
                     /locus_tag="KLK84_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HBE2008709.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1922..2374)
                     /locus_tag="KLK84_001320"
     CDS             complement(1922..2374)
                     /locus_tag="KLK84_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HBE2008710.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="KLK84_001321"
                     /gene_synonym="yicO"
     CDS             complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="KLK84_001321"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HBE2008711.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3936..5702
                     /gene="adeD"
                     /locus_tag="KLK84_001322"
     CDS             3936..5702
                     /gene="adeD"
                     /locus_tag="KLK84_001322"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBE2008712.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVMIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="KLK84_001323"
     CDS             complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="KLK84_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HBE2008713.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="KLK84_001324"
     CDS             complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="KLK84_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HBE2008714.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="KLK84_001325"
     CDS             complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="KLK84_001325"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HBE2008715.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="KLK84_001326"
     CDS             complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="KLK84_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HBE2008716.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="KLK84_001327"
     CDS             complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="KLK84_001327"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBE2008717.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="KLK84_001328"
     CDS             complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="KLK84_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HBE2008718.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="KLK84_001329"
     CDS             complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="KLK84_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HBE2008719.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13524..13613
                     /gene="tisB"
                     /locus_tag="KLK84_001330"
     CDS             13524..13613
                     /gene="tisB"
                     /locus_tag="KLK84_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HBE2008720.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13893..15077
                     /gene="emrD"
                     /locus_tag="KLK84_001331"
     CDS             13893..15077
                     /gene="emrD"
                     /locus_tag="KLK84_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HBE2008721.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15085..15582)
                     /locus_tag="KLK84_001332"
     CDS             complement(15085..15582)
                     /locus_tag="KLK84_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBE2008722.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15579..15941)
                     /locus_tag="KLK84_001333"
     CDS             complement(15579..15941)
                     /locus_tag="KLK84_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HBE2008723.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15931..16278)
                     /locus_tag="KLK84_001334"
     CDS             complement(15931..16278)
                     /locus_tag="KLK84_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HBE2008724.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VVVIVMGLVLYAG"
     gene            16387..16800
                     /locus_tag="KLK84_001335"
     CDS             16387..16800
                     /locus_tag="KLK84_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2008725.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16847..18289)
                     /locus_tag="KLK84_001336"
                     /pseudo
     CDS             complement(16847..18289)
                     /locus_tag="KLK84_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18324..20039)
                     /locus_tag="KLK84_001337"
     CDS             complement(18324..20039)
                     /locus_tag="KLK84_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HBE2008726.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20206..21099
                     /locus_tag="KLK84_001338"
     CDS             20206..21099
                     /locus_tag="KLK84_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBE2008727.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(21096..21910)
                     /locus_tag="KLK84_001339"
                     /pseudo
     CDS             complement(21096..21910)
                     /locus_tag="KLK84_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21910..23527)
                     /locus_tag="KLK84_001340"
                     /pseudo
     CDS             complement(21910..23527)
                     /locus_tag="KLK84_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
     gene            23822..24496
                     /locus_tag="KLK84_001341"
     CDS             23822..24496
                     /locus_tag="KLK84_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBE2008728.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24535..26196)
                     /locus_tag="KLK84_001342"
     CDS             complement(24535..26196)
                     /locus_tag="KLK84_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HBE2008729.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26392..26820)
                     /gene="ibpB"
                     /locus_tag="KLK84_001343"
     CDS             complement(26392..26820)
                     /gene="ibpB"
                     /locus_tag="KLK84_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HBE2008730.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26932..27345)
                     /gene="ibpA"
                     /locus_tag="KLK84_001344"
     CDS             complement(26932..27345)
                     /gene="ibpA"
                     /locus_tag="KLK84_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HBE2008731.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27339..27411)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27650..27982
                     /locus_tag="KLK84_001345"
     CDS             27650..27982
                     /locus_tag="KLK84_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HBE2008732.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27984..29203)
                     /locus_tag="KLK84_001346"
                     /pseudo
     CDS             complement(27984..29203)
                     /locus_tag="KLK84_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
     gene            29304..30404
                     /gene="cbrA"
                     /locus_tag="KLK84_001347"
     CDS             29304..30404
                     /gene="cbrA"
                     /locus_tag="KLK84_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HBE2008733.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30365..31702)
                     /locus_tag="KLK84_001348"
     CDS             complement(30365..31702)
                     /locus_tag="KLK84_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE2008734.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVVTLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31777..32925)
                     /gene="dgoD"
                     /locus_tag="KLK84_001349"
                     /pseudo
     CDS             complement(31777..32925)
                     /gene="dgoD"
                     /locus_tag="KLK84_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32922..33539)
                     /gene="dgoA"
                     /locus_tag="KLK84_001350"
     CDS             complement(32922..33539)
                     /gene="dgoA"
                     /locus_tag="KLK84_001350"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HBE2008735.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33523..34401)
                     /gene="dgoK"
                     /locus_tag="KLK84_001351"
     CDS             complement(33523..34401)
                     /gene="dgoK"
                     /locus_tag="KLK84_001351"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HBE2008736.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     VVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34398..35087)
                     /gene="dgoR"
                     /locus_tag="KLK84_001352"
     CDS             complement(34398..35087)
                     /gene="dgoR"
                     /locus_tag="KLK84_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HBE2008737.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35365..36021
                     /locus_tag="KLK84_001353"
     CDS             35365..36021
                     /locus_tag="KLK84_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2008738.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(36067..36879)
                     /gene="yidA"
                     /locus_tag="KLK84_001354"
     CDS             complement(36067..36879)
                     /gene="yidA"
                     /locus_tag="KLK84_001354"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBE2008739.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(36993..37391)
                     /locus_tag="KLK84_001355"
     CDS             complement(36993..37391)
                     /locus_tag="KLK84_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HBE2008740.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37631..40045)
                     /gene="gyrB"
                     /locus_tag="KLK84_001356"
     CDS             complement(37631..40045)
                     /gene="gyrB"
                     /locus_tag="KLK84_001356"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBE2008741.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(40074..41147)
                     /gene="recF"
                     /locus_tag="KLK84_001357"
     CDS             complement(40074..41147)
                     /gene="recF"
                     /locus_tag="KLK84_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBE2008742.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(41147..42247)
                     /gene="dnaN"
                     /locus_tag="KLK84_001358"
     CDS             complement(41147..42247)
                     /gene="dnaN"
                     /locus_tag="KLK84_001358"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBE2008743.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42252..43655)
                     /gene="dnaA"
                     /locus_tag="KLK84_001359"
     CDS             complement(42252..43655)
                     /gene="dnaA"
                     /locus_tag="KLK84_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBE2008744.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43952..44032
                     /locus_tag="KLK84_001360"
     CDS             43952..44032
                     /locus_tag="KLK84_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2008745.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44262..44402
                     /gene="rpmH"
                     /locus_tag="KLK84_001361"
     CDS             44262..44402
                     /gene="rpmH"
                     /locus_tag="KLK84_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBE2008746.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44419..44778
                     /gene="rnpA"
                     /locus_tag="KLK84_001362"
     CDS             44419..44778
                     /gene="rnpA"
                     /locus_tag="KLK84_001362"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBE2008747.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44742..44999
                     /gene="yidD"
                     /locus_tag="KLK84_001363"
     CDS             44742..44999
                     /gene="yidD"
                     /locus_tag="KLK84_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBE2008748.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            45002..46648
                     /gene="yidC"
                     /locus_tag="KLK84_001364"
     CDS             45002..46648
                     /gene="yidC"
                     /locus_tag="KLK84_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBE2008749.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46754..48118
                     /gene="mnmE"
                     /locus_tag="KLK84_001365"
     CDS             46754..48118
                     /gene="mnmE"
                     /locus_tag="KLK84_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBE2008750.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11992 a        13059 c        12206 g        11056 t
ORIGIN      
        1 gcggtaaggt cgctgcggtt taaaaaacga aacgctatcg ataagaataa aaaggaataa
       61 aagtggaata taaagtctgg cacttcttac tgaccacgca agcgcgtttc gtacagcatg
      121 atgagagcga tgaaagcaaa ctacacttat gcttcattcg atacacattt gtaaaaggtt
      181 gaatccatga aaccaacgac actactgctt attttcactt ttttcgctat gccaggcatc
      241 gtatacgccg agtcgccttt tagctcactg cagtccgcaa aagagaaaac cacggtatta
      301 caagatttac gtaaaatttg cacgccacat gcgtcattat cagatgaagc gtgggaaaag
      361 ttaatgttgt cagatgaaaa caataaacaa catatacgcg aggccatcgt ggcgatggaa
      421 cgtaataatc agagcaatta ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa
      481 gaacagcaac aactactgac caacgcttct ttacctgata tcaggatttc ttcattttca
      541 cctttgcagt aaccaacaat gcagtcagca acatcaatgt gccggacaac atcaacggcg
      601 aagtcagacc aatattatcc agcgcataac cgccgattgc cgcgccacag gtattagcaa
      661 gctgaataac cgccacctga atagacccgg ctttttctgc ctgatcggcc agcgagcggg
      721 tgatccacgt tgaccagccg acgggaacca atgcaaaagt tagcccccag ataatcgcca
      781 cgccggtagc aacgatttta tcgcttcccc acaacgtcag taccaacgca ctcacagcca
      841 gtattaacgg cgcgcctgct aaggccagtt ttaccgaacg tttaagaatg aacgacgaaa
      901 gcgacgtacc aataaagctg gcgataccaa aactcaacag caccagcgtt aagccatcca
      961 cgccgaatcc cgccaggttc atatacaccg ggcgaatata cgtgaagaaa gcaaactgcc
     1021 cggcgaaaga catgaagatg gcgatcatcc ctgccatcac acccggacgt tgtaataagc
     1081 ggaaagtatt ttgtttctga tgcgagggtt cgcctggcag tgaaggcaat gatttgataa
     1141 tccagaaaat acacagcacg cccatcaccg ccgccgcatt aaagacattg cgccaaccga
     1201 taagctcgcc taaaaaactg cccaacggcg cggcaatcac cagcgcaata gaaaccgcgc
     1261 cgaagatcac cgacagcgcc ttcggcaccg tacgcggcgg taccagacgc atggtcagcg
     1321 acgccgacat cgcccagaac ccgcccagcg ccagccccag acaggcacga ccgattaaaa
     1381 gcaaactgaa tgagttagca aaggaaacca gcaagcagga gagcgtcagc aaaacggcaa
     1441 acaaaataac aacgtagcgg cggtcagtgg cctgaattgt ctgggtaata aacaaactgg
     1501 caaacattgc cacaaaggcg gtcacggtca ccgattgccc ggcaacccct tccgaaatgc
     1561 ctaaatcctg ggccattggc gtcaacaaac tgacgggcaa aaactcaacg ataatcagac
     1621 aggcgacaca aaacgccacc gagaaaacgg ctgaccagtt cggtcgggtg atggcatccg
     1681 cgccgcggtt ttcggcaata aattcactca tggtgttacc cgtggcatgt ttcaggggaa
     1741 aagccgacca ctttaacatt gaaatgtgtg acgaatttaa cgtttttgca acttttacgg
     1801 tgttgcttga gctggagtta agatcgaaac ggaacagggc aaagattacc ggatgcggcg
     1861 tgaacgtctt atccacccga caggtcaggc gcggtctgta ggctgataag acgaaacggt
     1921 ttcaatgcaa ccagtgaacg ccctcggcag gctgaaagaa cgcgttatag cgcatctgaa
     1981 aagcattaat atcctgcatt tcaggttcgg tttcacgcag aaaacccagc gccagcctga
     2041 cctgagcatc ggtaatcggc gcggaaaggc gggctttttg taaaatccgg tgccattgca
     2101 gcaggttctg ttcgctatca tcgacaatga caatttgcca gataagcaac acctgcgcgg
     2161 cgttttgcag gtgcgactca tccgggcgat gcaaataacg taaaaattcg tcaccggctg
     2221 actttcccat ttgatcgacc atcgactcca cgggtacgac atcgatattc agccgatcgc
     2281 tgagacggcg aatcgctttt ctggccccgg atgtcagcac ccgaaaacca accacaaaca
     2341 ctaccgccag cgtggcgagc attatccaga tcatgagttc tcctgcgtaa tcagaactca
     2401 tgatatctgg aaaccctcgc cagttactaa tccaccagaa taatcttcaa tgcgaacaga
     2461 gctgcgacca ccaccacaca caggttcaga tcgcgccagc gcccggtgca cactttcatg
     2521 atgcagtacg acataaagcc gagtgcaatc ccttcggtga tcgagaaagt aaagggcatc
     2581 atcaccgtgg taataaacgc aggcaccgat tcggtaaaat catcccagtt aacgcgcgcc
     2641 aggctcgaag tcatcagcac gccaacaaag attaacgctc cagcggttgc gtaaggagga
     2701 accatcgcca ccagcggtga gaagaacata actaacagga acataacgcc gaccacgaca
     2761 gccgttagcc ccgtgcgacc accgactgcc acaccagaag tactttcaat ataggcagta
     2821 acagacgagg taccgataaa tgcgcctgcc accgaactga cgctatcaac atacagcgcc
     2881 ttattcatat tggggaattt accgttgcta tctattaagc ccgctttatc agttacacca
     2941 attaatgttc ctgatgaatc aaataggttg atcagcataa aggagaaaat gataccggcg
     3001 agttcaagtg ttaacgcgcc gctcaaatct acttcaccaa tgacgccgct aatatcaggc
     3061 ggaatggaat agacgccgct aaaatgaaca tcaccgaaaa ataatccaca gcaagacgtc
     3121 accacaatag aaaccagcac cgcggcatga aaatgacgtg atgacaacac ggttataata
     3181 aaaaacccta aaatacctaa caacacgccg tgagaactta aatcgccaat catcaccagc
     3241 gtgtctttat tggcgacaat aacaccagtg ttttttaatc ccattaaggc aataaataat
     3301 ccgattccac tggtgatacc aatacgtaaa cttaatggaa tgttggagat catccagtac
     3361 cggatacgaa acagcgtgag caaaaatagc ccaattgcgc cccagaatat cgcgcccatc
     3421 ccggtctgcc aggagatccc catcgccccc acgaccacga acgcaaagaa ggcgttcagc
     3481 cccattgccg gagccagcgc cacgggtaag ttagcaaata tccccatcgc aatactgccg
     3541 ataccggcaa tcaaacaggt ggtaacaaac accactttcg ggtccatttg tgccgcgccg
     3601 aggatttgcg ggttcacaaa aacaatgtac accatggtta aaaaagtggt catccccgca
     3661 atcaattctg tgcggacggt ggtcccatgc tgaggtagtt taaataatcg cgaaagcgtc
     3721 cctgattcat tactcacgta atcggtattg tcattattca tttttttgtc cattggagga
     3781 gatttaatcc cttgcttttg taaaacaaat gacaacaacg gttcagtgat aattatcaca
     3841 tttcaattgc acattaatag atattcttta ataatctcgc gacgtttctt tatgataaat
     3901 aataatcaaa ttgataaaat caaaatgaga aaaatatgaa taattccatt aaccataaat
     3961 ttcatcacat tagccgggct gaataccagg aattgttagc cgtttcccgt ggcgacgctg
     4021 ttgccgatta tattattgat aatgtctcta ttctcgacct gatcaatgcc ggagaaattt
     4081 ccggcccaat tgtgattaaa ggacgttaca ttgccggtgt tggcgcagaa tacgctgata
     4141 ctccggcttt gcagcggatt gatgctcgcg gcgcaacggc ggtgccaggg tttattgatg
     4201 ctcacctgca tattgaatcc agcatgatga cgccggtcac ttttgaaacc gctaccctgc
     4261 cgcgcggcct gacgaccgtt atttgcgacc ctcatgaaat cgtcaacgtg atgggagaag
     4321 ccggattcgc ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccag tacttacagg
     4381 tcagctcttg cgtgcccgct ctggaaggct gcgatgttaa cggtgccagt tttacccttg
     4441 aacagatgct cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa atgatggact
     4501 accctggcgt aattaacggg cagaatgcgc tgctcgataa actcgatgca tttcgctacc
     4561 tgacgctgga cggtcactgt ccgggtttgg gtggcaaaga acttaacgcc tatattgctg
     4621 cgggtattga aaactgccac gaaagttatc agctggaaga aggacgccgg aaattacaac
     4681 tcggcatgtc gttgatgatc cgcgaagggt ccgctgccag caatctcaac gcactggcac
     4741 cgttgatcaa cgaatttaac agcccgcaat gcatgctctg taccgatgac cgtaacccgt
     4801 gggagatcgc ccatgaaggg catatcgatg ccttaattcg ccgcctgatc gaacaacaca
     4861 atgtgccgct gcatgtggca tatcgcgtcg ccagctggtc gacggcacgc cactttggtc
     4921 tgaatcacct cggcttactg gcacccggca agcaggccga tatcgtcctg ttgagcgatg
     4981 cgcgtaaggt cacggtgcag caggtactgg tgaaaggcga accgattgat gcgcaaacct
     5041 tacaggcgga agagtcggcg agactggcgc tgtctgcccc gccatatggc aataccattg
     5101 cccgccagcc agtttccgcc agcgactttg ccctgcaatt cacgcccgga aaacgctatc
     5161 gggtcattga cgtcatccat aacgaattga ttacgcactc ccgctccagc gtctacagcg
     5221 aaaatggttt tgatcgcgat gatgtgtgct ttattgccgt acttgagcgt tacgggcaac
     5281 ggctggctcc ggcctgtggt ttgctcggcg gctttggcct gaatgaaggc gcgctggctg
     5341 cgacagtcag ccatgacagc cataatattg tgatgatcgg tcgcagtgcc gaagagatgg
     5401 cgctggcggt caatcaggtg attcaggatg gcggcgggct gtgcgtggta cgtaacggcc
     5461 aagtacaaag tcatctgccg ttacccattg ccgggctgat gagcaccgac acggcgcagt
     5521 cgctggcgga acaaattgac gccttgaaag ccgccgcccg tgaatgcggt ccgttacccg
     5581 atgagccgtt tattcagatg gcgtttcttt ctctgccagt gatccccgcg ctaaaactaa
     5641 ccagtcaggg gctatttgat ggcgagaagt ttgccttcac tacgctggaa gtcacggaat
     5701 aataaaaaaa gcccggcgtc atgcagggca aaagtcacca gttacgttta tgccactgtc
     5761 aactgctgaa tttttttctc gcggcggatt ttgcgttctt ccattaccgc cactatcgcc
     5821 atcaggcaga tacaaccaat cgcggcgata tccagcgcgg cgaaggtgcc tgcccagccg
     5881 gtaaggccga ataccggcgt cccatcggca atcattccca gacctaactt ggcaaagctg
     5941 tcaccaatca ggtaagcaaa ggtgccttta ataccatcgg cagcgccaat cgctttttta
     6001 ggtacaaagc caacagcagc cacaccaatc aacaattgcg ggccaaagac caggaaaccc
     6061 aacgcaaaga gagaagccag atagatatat tcgttactgg catgttgata cacaccgagc
     6121 gtggcgataa tcagcgccag cgcgatgcag gccaccaggc cacggcgacc gttcgccagg
     6181 tcagagagcc agccccacag cagcgtaccg accagcgcac cagcttcaaa cagcgtaaag
     6241 ccctgaatcg ccaccgcttt agagagtttc agttcctgga acgcgtatac ggttgaccac
     6301 tggtcgatac caatacgtac cacatagagg aaaatgttgg cgaagcacag cagccagatc
     6361 actttgtttt tcagcacata ctcaacaaag atctgccact tggtcatatc ggtagattct
     6421 gtctctttgt cctcttcgct gatctcctcg ccgaacagtt cttcagcttt gccgaggcca
     6481 taagattccg gggagtcgct gccgtaacgc aggccgataa aaccgacaat cagcgcgata
     6541 atcgacggga agataaacat gccgatgaca tggccatcga acaggtaatt tgccccgaac
     6601 agcgccacac ctgctgcgcc tgcaccgcca aggttgtgag aaatattcca gaaaccgagg
     6661 aatgtcccgc gtttacgacg cggcgtccat ttggtgatgg tggagtaact gcacgaaccg
     6721 ccggtactct ggaaaaagcc gcttaaggcg tagaaggcaa tcatcaggaa caggctaacc
     6781 gagccgctgc ccatactggc actgaagccc agcatacaaa tggcagagag gataagcatg
     6841 aacggcagga attgtttggt gtttttgccg tcggcgtagt atgaaaccaa cgttttaccc
     6901 acgccataag tgatggagaa acccaggccg atcatcccca gctgcgtcat gctcaacccg
     6961 taggtcgaaa tcatatcgtt ctgcgcgatg ttaaagttct tgcgaatcag gtacatcgtc
     7021 aggtagccga taaagaccac caggtaggat tgcatgaacg gtttgaacca cattttgcgc
     7081 cgcacttcga gcggaaggtc cagggtcggc ttgcgaacct ggtttaggaa agccagcatg
     7141 ggttactcct gaaatgaata cctgcgtgag gcatgcattg taaaaatcag cagcaaatca
     7201 ggcctgagat agcgtccaag aaaaacctgg aaaatttctt agttttgccg gatatgaagt
     7261 gaaaaggtga gatgcatcac gcttcacgcg gtgtctgggc gttcaaaaag ggcagtaaca
     7321 gcagtgcgga aatcccggcg gcgatagaga taaccacaaa aaatccgctc cagtgccagg
     7381 tatcgagcac tttcgccagc ggccaaccag caagcgacgc ccccagataa gcaaacaagc
     7441 cgacaaaccc cgtcgccgcc cctgccgcct ctttgtggga acactctgcc gccgccatac
     7501 caattaacat ctgcgggcca aagacaaaaa aaccaatggt gaagaagcag gttgcctgca
     7561 tcacgtagct ggcaaatggc atcagccaca gcgagccgac tgaaagcaaa attccggcgg
     7621 cgaaaatcaa attcatcggc ccacggttgc cgttaaacaa tttgtccgag ccccaaccgg
     7681 ctaccagcgc accgataaat ccgcccagtt caaacatcgt cactgccgta ttcgccgtga
     7741 ccagatcgac gcccagcgtc tcggacatat acaaattgcc ccagtcgttg atcgccgccc
     7801 ggaccacata gaccagcaca tagcaaaacg aaagcagcca gatatacgga ttcagcaaca
     7861 catatttggt gaggatctct ttacgcgtca accctgcccc ttcttgttgt tgagcaattt
     7921 ccagcgcgtc gtgccgccat tcaccgaccg ccggtaaacc taacgcctgc gggcgatcgc
     7981 gtagccgcca gcagagaaaa atccccacga ctatcgccat acaaccagca atcatcatcc
     8041 cggcacgcca gccgtaatgc agcgcgctcg ctgccatcac aatgggaatg agtgcgccgc
     8101 cgacgttatg cgccgtgttc cataatgccc accaaccgcc gcgctcggta cgtgaatacc
     8161 aggccgttaa cagacgcgca cacaccggtg aaccccagcc ctggaaaaag gcgttcagca
     8221 cccagagcac ggcaaacgcc catagcgacg tcgagaagcc aaacagaatg ttcatgatgc
     8281 ccgtggcgat aagccctatc cccataaaat aacgggcatt tgagcgatcg ctgacaatgc
     8341 cggagacaaa cttcgacacg ccgtaagtaa tgtaaaacag ggtcgctaac aggccgatat
     8401 cgctacggct gagcacgccg ttagcaagga tttctggtac cgcggcgtta aaacttttcc
     8461 gcgtgaagta aaacagcgcg taacccagcc agatggtcag cagaatatgc cgacgccagt
     8521 agcgataacg ggcatcaatt tcatgtttat cagtcattaa tggcgcatcg gcaggcgctt
     8581 tcagaaacgg caacatcatc gaaccttaga cataacgttg aggtagagaa acgctgacac
     8641 gcgtgccgtg cagacaggaa atgtgtaatg tgccgcccag cgccgttacg cgctcgcgca
     8701 ttccggtgag gccaaaacct tgttgcccgg aacccggcgg caaaccgctg ccatcgtctt
     8761 caatcaccag catcaaccgt tcatcctgct gccagccttg cagcgtaacc gcgctggcat
     8821 cggcatgttt cacaatgttg ttcagccctt cctggcagac acgaaacagc gtcacgcgct
     8881 ggttttcgct taatgctgat tcatcgatac gccattcgag atggctgaca atgccgcgcc
     8941 cttccagctc catttcccgc atcagtgagc ggatggcctg ctccagggtg agatcatcca
     9001 gctggcgcgg acgtaaccgt cctaacaaac ggcgcaccgc gtcgtaaacg cccagcgaca
     9061 gttgttcgat gagctgcccg ctctgcttca cactggcgtt atctgccgcc agccgctgaa
     9121 caatgcccgc ctgagtacga atagcagtga tggtctgacc gatatcgtcg tgcagctcac
     9181 gcgccacgtc gcggcgcacg ctctcttcgg tttctaacaa acgttcagcc agatgctgat
     9241 tgcgcgccag ttccttttgt agcgactggt taagttcacg caaccgctgg atgcctgcgc
     9301 ccagtaacaa ccctgtcaga ctttgcacca gcaacgagag taataaatcc accagatgat
     9361 cgcgccaggt ttgactggcg atcagcgcga tggcgttcat caacgtcgca atcagcgctc
     9421 cttgccagcc atagtgccag gccagcgcga taatcggcag cgccagacag aatggcgtaa
     9481 agcgcgacag ttcgtccggc aatcccaact ggagccagag actgataaca aacagcagca
     9541 agtaccagac cagatgccgc ccgcgccagt tgatgggctg agaaaccagt gacgggccga
     9601 gcggcagcca ggtgttattg gcgagatagt gccagaacac cagacatatc ggggccagcg
     9661 tcagaccgcc agttaaagtc agcaacagcg cattccacga ctctttgccg tgccaaagcc
     9721 agggcagcga ctgcaacaac gccgccgccg ttaacgccgc accctgtaac agcaaggtgc
     9781 gccagtcacg ctgatggcga tagcgcgaga tcagcgctac gggcagtaac gtcagtaaac
     9841 taccgatcat caataacgga aaatgggtta aaccgaccgc ctgcgttagc cagtaaatca
     9901 gcagccactc cgcacccagc aatacgggcc agtatccgcg cgggcattgc agcattagcc
     9961 ccagacgcag accaaacgga aataacagca ccgccatatc agggcgctca accagatgca
    10021 ggctgatact ccataggcaa aaccatgcgg cagagaagat aaaaaagcag gcaataacgg
    10081 taattaagcg ggagaacaac gtcttcatca ccagccatca aacatgcggc gcgccagctc
    10141 tacgtcgtta ctgacgccca gtttttccat cagattggcg cgatggacgt gtaccgtttt
    10201 cggtgacaag cccagttcgg cggcaatctc tttcaccgcc attccttgcg ccagtttttc
    10261 cgccacctgg cgttcacgtt tggtcagcgg gtcctgacga ccggatgcca gtttaatggc
    10321 aatatccggc gtcagataac agccgcccgt ggcaaccgta tgcaccgcag caatgagttc
    10381 atccgggcta cagcgtttgg aaagaaagcc gcgtgccccc gcgttaagcg cctgctcaac
    10441 cagcgcagga ctgtcgtgaa cggagagcat aatcgtcgcc atacctttcg gcagctggct
    10501 tagcagctcc agaccggaga tatcgggcat ggagatatcg caaatacaca cctgcacacc
    10561 gcgccccggc agccccgcca gcgcctcgcg ccccgaacca aactcggcaa ctacctgcaa
    10621 atcaggttcc agccccagca gctgcgcaaa gccggagcgg acgatgaggt gatcgtctat
    10681 aagggcaacg gtgatcatgg tcttgtcctg gcgggtaaaa aaaaacgcgc ttaccttaac
    10741 gataagcgcg atgttgttca agccttgagc ggttactgaa aaaacaccgc gatcttgtta
    10801 aacatcgtcg gatcggactg attacgctgc actttcacga catcttccag cttagcgatt
    10861 tggcttatca tctgctccag acgctggtcg tcattgacca gtagccagat atggcttttg
    10921 tcgctgtcct gaatcggcag acaaagaatg ccttcaacgt taaaagcgcg gcgggcaaaa
    10981 aggccacaaa cgtgggtcat tacgcccgga tggttgcgaa cggtgagctc cagaattacg
    11041 ttgttatgag ttgtgttttg catggcttat tcccccacca tttcagtatt cgccgcacct
    11101 ggcggcacca tcgggtaaac tttttcttcg gcatcaatgc gcacatggat cagcgccggg
    11161 ccagggcgat tgatgatttc ctgcaatgca gcctgcggat cggcttcgtt attcaaatca
    11221 caggtttcga ggccgaatcc ggcggcaatc tgcataaagt tgattttgcc cggataggtg
    11281 gcggcaaaaa cgccttgctc gtagaacaga ctctgttgct gatgcaccag ccccagcgct
    11341 tcgttgttca tcagaatgat tttgacatcc agctgatttt cactggcggt cgccatctcc
    11401 tgaatattca tcatcaggct gccgtcgccg gagaaacaca acactttgcg atccgggttc
    11461 gccagcgccg cgccaatcgc cgcaggcagg ccaaaaccca tcgtgcccag cccaccggag
    11521 gtcagccact ggcgtgggcg attgagcgga taagcttgcg cggtccacat ctgatgctga
    11581 cccacatcgg tggtgataat cgcattgtca tcgacacagg cggcaacggc gttgatcagg
    11641 ccgtaatggc ttaatggatc gcacgctttc gggattggac acgggaattc gcgctgcaaa
    11701 tccgctacca actggtgcca ctctgcacgc ggttgcgctt ccacccgcgg gatcaattgc
    11761 gccagcacgt catcaacatc cgcctgaatc gccacatgcg gctgcttgat tttacccagc
    11821 tctgcgcgat cgatatcgac atgaataatt ttggcattcg gacagaactg ctcggttttg
    11881 ccaatcgccc ggtcatcaaa acgcgcaccg agcacaatca acaaatccgc ctcctgcaaa
    11941 atatagttgg tactgcgcac gccgtgcatc cccagcatac ccagcgacaa cggatgcgct
    12001 tttggcagca tgcccagcgc cattaaagtc atggtggtag gcagttgcgc tttctccgcc
    12061 agttcacgca cccgcgcagg cgcattgatc acaccaccac ccagataaag caccgggcgt
    12121 ttggcagcgt taatcattgt agctgcgtca cgaatgcttt cttcgctaaa ggcgggtgca
    12181 gcggcttttt ctgccaccgc gggctgagct tcaatctcaa aaaccgccgt ttgtacatcc
    12241 ttaggaatgt ctatccacac cgggctaggg cggcctgatt gcgcaatgcg gaaggcatcg
    12301 ctcatgacct gtgggagctc ttcgatatgt ctgaccagat agttgtgttt ggtgatgggg
    12361 atagagatgc cgtaggtgtc cacttcttgg aaggcgtcgg tgccgatcat cgaggcggga
    12421 acctgaccag tgatgcaaat cagcgggatg gagtccagcc gcgcatcagc aatggcggtc
    12481 accaggttag tcgcacccgg cccgctacag gccatacaga ccgccggttt accgtcggtg
    12541 cgcgccattc cctgagcgat aaaccccgcg ccctgttcat ggcgagccag aatatggcgg
    12601 atttgcgtgc tttggcttaa ggcgtcgtaa acaggcagga tagaaccgcc cggaatgccc
    12661 gtcacaatct taatgccctg ctgttccagg aaatgaacga taaattctgc gccggtaaag
    12721 cgcttacgcg tcgatgttgt gcccgaactt gccatgctcc agtccttttc ttctgggccg
    12781 actttccggg aggtgcttaa acgaaaaacc ccgcccggtt tgcgccggcg gggttttgga
    12841 atcgtgtgtt gttccagtcc ctacggcgca ttgccgacga ccaccaccac acgcacgacg
    12901 accactgcgg cggatggcgc agttggtagt agttttgcgt tgaacatgga agtagtcatt
    12961 ggggaccttg ttggttttgt gtttaacaat atttatacaa gcacagcttt acaggggaga
    13021 caatggaaaa tttttcagca agggaaaatt gaggggttga tcacgttttg tactgaattg
    13081 cagataacaa aaaaccccgc cggagcgagg tttcgtcagc cgcctgcggc tggtaaccgc
    13141 aaagcacact gtattatgtc aacactgaaa gtatacgtgt tccgcgcaga acgcgcaatt
    13201 tcggcacgaa ttttgacgta tttagtgcat agttgagtat cgttcacagt ttgcgttttg
    13261 tccaaatatt actgtttatt tatacagtaa acttctataa tatcactgta cgcaatgtgt
    13321 tatgcggggg ccgcatcgtt acccggcgca ctaagtcctg gctgaaacgg gtggtgccgt
    13381 cagcgcctta accccgcgtg agcacactgt gttatgtcaa caagcacaac gtttctcctt
    13441 gagataccgc gtgcacaaca gctggcaaca ggcagcggaa aggtacgtca gctggcagtg
    13501 ctcctgaacc acaggagacg cgtatgagcc tggtggatat cgccattctt atcctcaaac
    13561 tcattgttgc agcactgcaa ctgcttgatg ctgttctgaa atacctgaag taattcagat
    13621 tcaagtcgca ccaaagggga gcgggtaacc gctccccttt tatatttagc gtgcgggttg
    13681 gagtcggatg cgatgctgac gcatcttatc cgccctacca tccctcccgg caacatttat
    13741 tgccgctttt gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg
    13801 ctaacattca tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta
    13861 ttctaattat ttttataaaa gcatccgtga taatgaaaag gcaaagaaac gtcaatttgt
    13921 tattgatgtt ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag
    13981 ctattgccga tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag agcgtaatgg
    14041 gcgcttatct gctgacttac ggtgtctcac agctgtttta tggcccgata tccgaccgtg
    14101 tcggtcgccg accagtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg
    14161 cggtcacgac ctccagtttg acggtattga ttgccgccag cgcgatgcag gggatgggca
    14221 ccggcgttgg cggcgtaatg gcgcgtactt tgccgcgtga tttatatgaa cggacacagt
    14281 tgcgccatgc taacagcctg ttaaacatgg ggattctcgt cagtccgttg ctcgcaccgc
    14341 taatcggcgg tctgctggat acgatgtgga actggcgcgc ctgttatctc tttttgttgg
    14401 ttctttgtgc tggtgtgacc ttcagtatgg cccgctggat gccggaaacg cgtccggtcg
    14461 atgcaccgcg cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta
    14521 actgttattt gctgatgctg attggcggtc tggccgggat tgctgccttt gaagcctgct
    14581 ccggcgtgct gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta
    14641 ttctgccgat tccggcggca ttttttgggg catggtttgc cgggcgtcct aataaacgct
    14701 tctcaacgtt gatgtggcag tcggttatct gctgcctgct ggctggcttg ctgatgtgga
    14761 tccccgactg gtttggcgtg atgaatgtct ggacgctgct cgttcccgcc gcgctgttct
    14821 ttttcggtgc cgggatgctg tttccgctgg cgaccagcgg cgcgatggag ccgttcccct
    14881 tcctggcggg cacggctggt gcgctggtcg gcggtctgca aaacattggt tccggcgtgc
    14941 tggcgtcgct ctctgcgatg ttgccgcaaa ccggtcaggg cagcctgggg ttgttgatga
    15001 ccttaatggg attgttgatc gtgctgtgct ggctgccgct ggcgacgcgg atgtcgcatc
    15061 aggggcagcc cgtttaagcg cacgtcaccg cagcatcgtc atcagctcca tgggagaacg
    15121 atgctgcttt atcagatcac gcatcacccg catatgcggt gcggagtaag aataaaacgc
    15181 ctgatagccc gcacaaagca cgctgtctgg cgtctcctgc cgagcgtgcg ggcacaggcg
    15241 atgccagtcg cacgcctggc agtcacggcg cggttgcggg ttttcgctgc cacaccattt
    15301 ttgtaaggtg gcgtcaaaaa gctcaatcga gatacgactg atatctgccc gcacccaaca
    15361 ctcaaacagg gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag
    15421 atcgccccgg tcatcaaatt caagataggg aatgagctgt acggagcgga tgccccgctg
    15481 tttgaaattg tgataaaaat cggcaggttg cagagcatcc ttgtactcta caaccagctt
    15541 atgcctgtcc tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat
    15601 acgataagtt gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg
    15661 gagatcaaaa atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc
    15721 attaaattgc gattacgggt gtagtgccag aggatcagcg ccacgatggc gaggatgcca
    15781 atcgaaatcc agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc
    15841 aacgcgccgt agcccagcat ggtacgaaac cacgccagtg atgtgcgctc cggctgcagc
    15901 cctgggtcgg caatgcgtct ggctttacgg ctatccggca tacaacacca gtcccataac
    15961 gatcaccacc acaaccatca atattaagct gatgattaat aagctgttgg tgtagggcag
    16021 gtcctctttc agacgcatcg ctttttcatt gcgtaaccag cgcaggtagc catacattgc
    16081 taacccgccg gaaaacagac acaacaacaa cgccagcagt tcacgaatga cgggcgtggc
    16141 gaaatctggc gcaagttgat caagcccaac ccccgccgcc agaaatccta gtgcggtacg
    16201 gatccacgcc agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc
    16261 aaggcgggaa attttcattg ttgctccttt gcaatttcac taacgctcta tctttagcgg
    16321 ttttttttaa gaatttcgtc cgcccgtttc ctgatatgct tttgccatcc gctacaagga
    16381 ggtcagatgg gtatcatagc gcaaaataaa atcagttcat tgggcatgct gttcggtgcc
    16441 atcgctttaa tgatggggat tatccacttt tcattcggcc ctttttccgc accaccgcca
    16501 acgcttgaaa gcatcgtggc ggacaaaacg gctgaaatta aaagaggtct tctcgccgga
    16561 ataaaaggcg aaaaaattac cactgtggag aaaaaagagg acatggatat cgataaaatc
    16621 ctcgatcaaa gcggtatcgc actggccatt gctgcgctac tttgcgcatt tattggcggt
    16681 atgcgtaagg aaaatcgctg gggcattcgc ggcgcgctgg tttttggtat cggcatcgtg
    16741 tgcagcattt tgctgatatt cctgattttt tccttcctga ccggcggttc gctggtttaa
    16801 gcagcctgaa taatgcccgc cgggtgatca tcagcgggca ttatcgtcag aatttctgtt
    16861 ttttctcctc caccttcact ccttgcgtcg gtaggccggt gtcatagtcg cgtaccactg
    16921 gcgaatagcc atcttgtggg cgtgggcgaa acgcccccat ccagcgcggt cgtgcatctt
    16981 tacgccacgg acgcagactc cattggtaac tgcggaacgg atcgcgaatt ttgtccatgt
    17041 aatccaataa ggcgtcatgc attttgctgc gaacgtctgc aaaacggata tcatcgatca
    17101 ggttatgcat ttcatttggg tcattacggc gatcgtaaag ttcatcactg gtgaagaggt
    17161 tgagcaccag tttaaagtca tccgtcaccc agcaacgcac cggaataaaa ccgccaaagc
    17221 tgtcatgctc aatctcgtag cggttaaatt ccaccatcac gccgcgtggc tctttcacgg
    17281 caaggatatt ttcccccggc agaatctctg gtttttcaat atctgccagc gccatcattg
    17341 tcggcagtaa atcaatatga ctgactggcg tatcgacctg tcgccgctcc ccttgcggcg
    17401 aacggatgat cagcggaata cgggtgatgt cgtcatacat cgccgctcct ttactgatca
    17461 gcttatgtgc gcccatcatt tcaccgtgat cggaggtata aatgacccac gtattttcac
    17521 gttgctctgg cgttaaggca ttgatgacac gtccgatttg gtcatcaaca aagtcattgc
    17581 aggcaaaata gagcggatgg tgataaagcc cgtcatcacc gactggcgat ggcatcgcct
    17641 gcgcccataa gcggtgatgt tccggtttgt tcgccaggtc atcctgtgat ttctcgccaa
    17701 gttcgtagta aaaatcagcg tatttctcta aatactccac cggacaggtg aacgggtgat
    17761 gcggctcatc atacgaaacc accatcagga agggttcctc ggcgcgcacg ggctattgca
    17821 gaaaatctac cgctcgattg ctgatgcggt gcgcccaggt gaaggtttcg tcaatatggt
    17881 tcgcctgtaa atcttcgacg ctgtttaggc cattgcgcca caggctgatc tctttttccg
    17941 tcagttcgct aagatagttc gccccatcaa accagtaatc agcatcccac tccggcggac
    18001 actcgccagt gccgaaatag tcatgaccgt cgagatgcca tttgccgatg taacaggtgt
    18061 gatagccggc atccttaaag tagcgcccca tagtggagat gtttttgcct ggcgcgacgt
    18121 tgttggtcca cgggccggac tggttagcgt agataccggt gaatagtccg gcccgtgcag
    18181 gcgtacaaac cggtgaacaa gtgtaggcgg aattaaagcg aataccttcc gccgccagac
    18241 tatcaatatt ttgcgtattc agcggtttac cgctatagca accgaccata ttggtggcct
    18301 gggtatcggt caattggggc gtttcatagg ctatccttcg cgtcgggagt aaaggttacg
    18361 gctgggtcgt gacgatgcca ccagctgtca aaaatcaggt agacaatcac tacggcagcg
    18421 atgaaataac tgatcatcgc taaccagcgc gtaccgtagc cgccgaattc agccagcccg
    18481 acatagacgc caatcatcgc gaacaggata ccaattgacg cgatcttgac gtttttccac
    18541 ggtttcatgt ccaccgcaaa cgcatctttg aaggtgaacg gcgtggcgcg cggtttgata
    18601 aaaccgatca ccagcatcac gaccacgttg atgcagaacg tacaggccag cacatagagg
    18661 aaatggaagt cgaacttcac cagatagttg atggtgatgt agctgattat gccaatcccc
    18721 atcgccactt ttgccgccag cgccgggatg cgcggaaaga aaaagcccat aatgatgatg
    18781 gtaaccagcg gcacgttgta aatgccgttg agctgtttca tccagctata cagcccctgc
    18841 ggcgcgttgg cgatccacgg cgctaccaaa accgaaacga tagcgataaa gaaaccaaat
    18901 ttgcgcccga cggtgaccag ctgctgcggc tcggcattct ggttaatgat gcgacggtaa
    18961 atacccatac tgaataatgt actggcgcta ttcagaaagc cgttgaaggt actgatcacc
    19021 gcaccaaata acaccgcgcc gaagaaaccc tccagtggca ctggcaaaac gttattgacc
    19081 agcgtcgggt aggccatgtc ggctttcggt agatcctgat acagatgaaa cgcaatcaac
    19141 cctggcagca ccagtaccag cgggtcgagc attttcagca ccgccgttaa cagcgccccc
    19201 ttttgccctt ccgccaggct ttttgacgcc agcgtgcgtt gcacgatgcc ctgatttgta
    19261 caccagtaaa aggtgttcac cagaattaaa ccggtaaatg ccgcgccaat cggcaaggga
    19321 tcggtcgggc caccgactga gtttaatttc tcggcgtgaa cggtggtaat ttgctcaatg
    19381 ccctgcataa agctgccctt gcctatcgcg atcaggccaa ataccggcac catcaacccg
    19441 ccgataacca gcccaatacc gttgatggag tccgccactg ccattgcgcg cagtccgccg
    19501 atcaccgcat acaaaattcc cgccagacca agcaaaatta ccagtagcca gatagccgca
    19561 ccgtgagaaa tctgtagcga ttccccgacg tgaaacaggc tgttgagcgc caacgcgccg
    19621 gagtagagaa caatcggcag aaagcagacg ccggtggcaa tgaggaagca gaagtcgatg
    19681 ataatacgcg tcgttttatc ataacgttcc tccagaaaat cggggatggt ggcaatgccg
    19741 cgcttcagat agcgcggtaa aaagattagc gcgaggaaga tcagcgtcac cgctgaagtc
    19801 acttcccagc ccatcaccga catgccgctt ttgtaggcct gcccggaaag accaaccagt
    19861 tgttccgtgg aaaggttggt taacattaac gaagcggcaa taaccggcgc ttttagtgaa
    19921 cgtccggcaa gaaaatagcc ttgttgcgat ccggtatctg ttttgcgaac cttccaccag
    19981 gtgatcaccg ccaccagcag cgtaaaaccg acaaaactca agatttgtaa cgaattcatc
    20041 tcatagccct tatttatgtt tatgtcccgg acgcatgaat atagggagag gtaaaatgcg
    20101 tcgggtcagg cagtcagatt cttaaaatac aaacgtcgta tccctgaacg gattgttttc
    20161 tgttaagttc aggttgtgag cataattcgc cggagggata ttaaaatgaa tggaaaattg
    20221 caaagttcag atgtaaaaaa cgaaactccg tacaatattc ccttattgat taacgaaaat
    20281 gtgatctcca gcggaatttc tctgatctcg ctctggcata cctacgccga cgagcattac
    20341 cgggtgatct ggccgcggga caagaagaaa ccgcttatcg ccaactcatg ggttgcggtg
    20401 tataccgtac aaggatgtgg gaaaattctt ttaaagaatg gcgaacaaat aactctgcat
    20461 agcaactgta ttatattttt aaagccaatg gatattcact cttatcactg tgaaggttta
    20521 gtctgggaac agtactggat ggaatttacc cccaccagta tgatggatat tcccgttggt
    20581 cagcaaagcg ttatttataa tggcgaaatt tataatcagg aactcaccga agttgctgag
    20641 ttaataactt caccagaagc aataaaaaat aatctggcag tcgcttttct gacgaaaatt
    20701 atttatcagt ggatttgtct tatgtccgca gatggtaaaa aagatccaca acggcggcaa
    20761 attgaaaaat taattgccac tttacatgcc agtctgcaac aacgctggag cgtagctgat
    20821 atggctgcca cgatcccctg tagcgaagcc tggttgcgtc gtctgttttt acgctatacc
    20881 ggcaagacgc cgaaagaata ttacctcgat gcgcgtctgg atctggcgct atcgctatta
    20941 aagcaacaag gaaactcggt tggcgaagtc gctgatacgc tcaacttctt cgactccttt
    21001 catttcagca aagcctttaa acataaattt ggttatgcgc cgtcagccgt gctaaagaat
    21061 acggaccagc acccaacgga tgccagtcca cacaattaac gtaactctgg ccagtacgct
    21121 ttgttggcct ccagcaactc atccagaata gctttggcga ccgaggcgct cggtaccgtt
    21181 ttcgacaacg taatcgcctg ccacaggtgc tgatatgaac gctgttccca ggcgtccacg
    21241 accagttttt ccaccgccac ttgctgactc attaaccctt tctgaaactg cgggatatcg
    21301 ccgaccacca gtggttcagg ccgtagtagc gcacgcgcat ctgtttgcga tcttgcagcc
    21361 caacaatttg cgccatccgg ctttcaatac cgattggcat gtcacagatg ttgaggattt
    21421 tcgcattcgg gcgcagacga cgcgtggctt ctgcgacaat tgctgccggg ttggagtagt
    21481 tgagcatcta ggcgtttggt gaatattttt ccatataatc caccagttcc aggacgccgc
    21541 caatggaacg catgccgtac gctattccgc cgggtccgca agtttcctga ccaacaacac
    21601 cgtggcgcag tgggattttt tcatccagtt cgcgcatcgg gtacttgccg acgcggatgt
    21661 gcgccatgac aaaatcaacg tcgctgaacg ccacttcagg atcggtggtg taactaaacg
    21721 caatgtccgg cgctttttct ttaaggatga ctttacaggc ttcggcaatc acttcctgcc
    21781 gcgcgccatc gttatcataa aatttcagtg cacgaagcgg gaaacggtcc tgattcgcca
    21841 ggagcatcaa cacgatgcct ggcgtaaagg tgcttccacc gcctgcgaca accactgaga
    21901 atttggtcat tatactgcct ccgtaatggc aacattttct gctgattgat gagaattaat
    21961 taagctatcg agctgttcac gcaattggga tacatgcaga ccaatgatca cctgaatggc
    22021 atcgccacta cggaagacgc cgtgcgctcc cagcttttta aagacttcgt catccagcgt
    22081 ttgtgacatg tcatgcagtg caatacgtag acgcgtcgcg caattgttaa tgctggagat
    22141 attgccgacc ccgcccaggg cttgcaggat accggcagcc tgatccagct ctttttttgg
    22201 ctctgcagcg gtggtttggc ctcgcgaggc tttgtattcg gcttttgagt agagtttcac
    22261 ttccgcatct tcacgtcccg gcgtgcacat gttgaactgc agaatcaggg tgcggaaaac
    22321 cacgaagtac agcagggtaa agcacaaccc aatggcgatt tgggtcagca tcatatccgc
    22381 gtggttgctg aacatcggga tccagttttg cggtaaaacc tggtcaatca gacctccgcc
    22441 catgttgccc accacaccaa agagatacat tacggtcgac attgaggccg ccagcacggc
    22501 gtgtaccgca aacagcaacg gtgaaatgaa caggaaggta aattccagcg gttcggtaat
    22561 tcccaccagc atggcggtta aggttgcggg aatcagcaag cccgccactt ttacccgatt
    22621 ttccggtgcg gcagtgaagt acatcgctaa agaaatgccc acggcaccaa agatttttga
    22681 gttaccgtgc agggcaaaac cgccttccgg gaacaacgat ttcagtggct cggcgctcag
    22741 actgaactct tgcagatgct gcgcccagta catctgaatg ccgccttcaa cagctgccgg
    22801 accaaagata aacggtccgt agatgaagtg gtgtaaaccg gttgggatca gaatacgttc
    22861 gaggaaggtg taaacccaca ccccaagcgc acccgccgaa cgcaggaacg cttgcagaga
    22921 ttcaatcccc atttgtactt ttggccagcc gagcaatgtc agccaggcac aggggatcat
    22981 caccaggaag gcgataatca ccacataaga cgtcccctgg aaaatgccga gaaaaaccgg
    23041 cagtttttta tcgaacagac ggttatgcag cgccgtcaca atgccggaaa tgataattgc
    23101 gccgataatg ctggtatcga gggttttaat cccggccatc attgtcagac cgctacctgc
    23161 caccgcgtcc tgagtgaaat cgacgccgaa gtagcttccc caggtcattc ccatcgcatt
    23221 gatgaaatag ttccaggtca ggaaactcac catcaccgcc agacaagcac gtccctgcgc
    23281 ttgcttagca aggccaatgg gtaaaccgac agcaaaaatc agcggcatat tacggaatac
    23341 cgtccaaccg ccctcttcaa taatgtgtac gatttgcgcg aataaactgt tcggatcggt
    23401 cagtgattcc ccgacaaaac atcgggtttt gcagcaagat ggcaagaccc accacaatcc
    23461 cggcgaaggg aaacagcagc actggcgtga acatcgcgcc gccaaagcgt tgaatttgac
    23521 tgagcatttc cacatcctct tttctcaatt ctgtaagtag gaagcctgat tggttatgcg
    23581 gcagacctgg agcgaggata ggaagatgcg atcggcagaa catgacgacc caatgggatt
    23641 cgtgatcgct ttcatgcttt tcgttgggcg gtagagatct acgttttcgc gaaaaagtag
    23701 acatctgtga gcacaggtac acagcatcga acacatcttt aaaaaaaaga tttttttcaa
    23761 tcgattaagc agaacttgtg ggcgcattac ccgtgcttgc aggcaaaaaa gagagctaga
    23821 gatgatctac aaaagcattg cggagcggtt aagaattcga cttaattccg cggatttcac
    23881 gctaaacagc cttcttcccg gtgaaaaaaa gctggcggaa gagtttgcgg tatcgcggat
    23941 gactatccgt aaagccattg acctgctggt agcgtggggg ctggtggtcc gccgccacgg
    24001 cagcggcact tacctggtgc gcaaagatgt gctgcatcaa accgccagcc tgaccggact
    24061 ggtggaggtg ttaaaacggc agggaaaaac ggtcaccagc caggtgctga tttttgaaat
    24121 catgcctgcg cctccggcca ttgccagcca gttacggatt caaatcaacg agcagatcta
    24181 cttctcccgt cgcgttcgtt ttgtggaagg gaaaccgcta atgctggaag acagctatat
    24241 gccggtaaaa ctgttccgta acctttcgct gcaacatctg gaagggtcga agtttgaata
    24301 tattgaacaa gagtgcggga ttttgattgg cggtaattat gaaagcctga tgccggtgct
    24361 cgccgataga ctgctggcgc ggcaaatgaa ggtagcggaa cacacgccac tgctgcggat
    24421 cacctcgttg tcatatagcg agagcgggga gtttttgaat tattcagtga tgttcagaaa
    24481 tgccagcgaa tactaggtgg agtaccattt acggcgactc cacccggaaa aaagttaacc
    24541 gatactccag aagagcaccg ccagtaattg gggggtgata atgcgcagga acatcactaa
    24601 cggatagaca gtggcgtaag agagcgccgc cgcaccactg gttggatgaa gattattagc
    24661 aaacgccagc gccggaggat cggtcatgga acctgccagc atcccgcaca tggtcaggta
    24721 attcattttg gctaacatcc gcgccagaat gccaacagta atcagcggaa cggcggtgat
    24781 cagggcacca taaccaatcc agcttagccc ttcgccattg accagggtat tcacaaaatc
    24841 cccaccagat ttcagaccaa cgaccgagag gaacagcacg atccccagct cccgcagcgc
    24901 gaggttggca cttggcggca taaaccagta cagcttgccg atactgccga tacgcccgag
    24961 gatcaacgcc ataatcagcg gaccgcccgc cagccccagt ttcaacgcgg ccgggaatcc
    25021 tggcacaaag acgggaatag aacctaacaa tacgccaagc ccgatgccaa taaacactgg
    25081 cagcatctga acctgttgca gtttttgctg cgcattcccc agcacattgg caacggcatc
    25141 aattgcggac ggacgcccca ccaggttgag gatatcgccg aactgcaggc tgatatcgcc
    25201 actggcgacc agttcgaccc cggcacggtt caggcgcgag ataacaacgt catagcgttc
    25261 tttaaagtgc aggtcgcgaa tacgttttcc gagcacgttt tcattggtga ccaccacacg
    25321 ctcgacgcgc aaatcagtgc ctttcgtaga cagcgaggta tcgacctcct gaccaatcac
    25381 cagttgcgca ttatgtaaat ccgctggctg ccccaccaga tgcagcaaat cgcccagttg
    25441 gataatggta tctggcgaag gtacttttag ggtttcttct cgtttcagac gcgagcagat
    25501 aattttgtcg ccgttgagaa tcggtacatc tttaatggct aaatcatgca ggttagggtt
    25561 ctcaacgcga atattgatag tcttgatcaa cgcgccgcca ttggtgcgtg aagactcgtg
    25621 ctgctgagct tctgtctcga cattgacgcg gaaaataacc cgcaacatcc acatggtgaa
    25681 caaaatcccg caaatgccga atggatacgc catcgcgtaa ctcatcccca tctgatcgac
    25741 catttccatt ggtgtaccca ggtcgcgcaa gatctgctgc cctgccccca gcgctggcgt
    25801 attggttacc gcaccggaga aaatccccag cactaccggc agtggaatat caaacagttt
    25861 atgcaggatg gcggtaacca gaccaccgat gatgacgatc agaacagcaa acaggttgag
    25921 gcgtaatccg gagacgcgca atgaggcaaa gaagcccggc cctacctgaa tcccgatggt
    25981 ataaacgaac aggatcaggc caaactcctg aataacatgc agcatatcgc tacttaatgt
    26041 catccccgcc tgagaaacaa aatggccgac gatgatccca ccaaacagca cgccgccaat
    26101 acctaatcct atgccgcgaa atttgacgtt gccgataaac aaaccgacga ctgccaccaa
    26161 agccagaata ctgaccgtta atgctatatc actcatcgtc catttctctt gagataacat
    26221 tttaattgct acggattatg gctgaatcgg tgaagcagat ttgccttata gcgcacatta
    26281 tcacgggaaa gaacgtgccg aaatatctta aacagtcgaa atttaacgca ttgttgaaga
    26341 agaaagacaa aaaaagcccc accagaatgg cggggcaaag agaatagcta gttagctatt
    26401 taacgcggga cgttcgctga tagcgatacg ctgcgctgcg atgggttcag gctcattacg
    26461 aattaaatca atatgcagta aaccgtttac gaaggttgcg ccagagactt ccatattttc
    26521 agccagcgta aaactcaggc taaatggctg attcataagc ccttgatgca gccatttttt
    26581 ctcttctttt ggctgctccg gcgtgccttt tacgctcagg cgcgtacctt ccagttgaat
    26641 ctctaaatct tcctgacgga aacctgccag cgcaagggta atgcggtagt ggttatcgtc
    26701 gcttttctca atgttgtacg gcgggaagct ctggctttca ccggcgtttt gcagtgcgtt
    26761 ggccagtttg tcaaaaccga tccattgacg catcagtggg gataaatcga agttacgcat
    26821 agtcatttct ccttctaaga agcgagtaag tacctgcaaa tccgaagatt cgcatatgct
    26881 ccctgacggc gagcatggag atgtcaggcc gcgccaggcg gccttaggga attagttgat
    26941 ttcgatacgg cgcggttttt tcgcttccgg aatcacgcgt tcgagatcga tatacagcaa
    27001 accatttacc aggttagccc cacgaacatg aatgttctca gctaactgga atttgcgttc
    27061 aaagttgcgt tcagcgatgc cctggtacag ataggtgcgc tctttttgtt cgtcggcgtg
    27121 agcacctttc accaccagca gattatcctg ggcggtaatt tccagttcgc tctcagcaaa
    27181 accagccaca gcgatagcaa tgcggtaatg gttttcgtct accagttcaa cgttatacgg
    27241 agggtagccg ccattactct ggctctggtt gttttctaag tggttaaaca aacggtcaaa
    27301 tccaatagca gaacggtaaa gcggggataa atcaaagtta cgcataatca atagctcctg
    27361 aaatcagcga gaatgtaaga ccttccacaa tggacaggtc aggtagccag aacacccatc
    27421 aggcgcgtcc tcatcgacta cgatgtaaaa tgggtctgga aatgaacttt tcaagcctta
    27481 tcagcggact tttttttgca gtttatggtc tattgcatag actgaggggg cagcacgaaa
    27541 cgttaaagtg cgatagccgc cacagtgcga cgtaatgatg atgttatttt cagcaaggga
    27601 tcgctataac tcatcaggcc aacacccacg atcaacagat gaatttatta tgataagaaa
    27661 tgtgttgtta gcgttcatga tatgcagcgg aatgacatta ctcggaggat gctccagcgt
    27721 gatgtcccat accgggggga aagaaggaac gtatcccggc acccgcgcca gcgccaccat
    27781 gattggcgac gatgaaacca actggggcac taagtcgctg gcgattctcg atatgccgtt
    27841 taccgctgta atggacacgc ttttactgcc gtgggatgtg ttccgcaaag acagctcggt
    27901 gcgctcgcgc gttgaaaaaa gcgaagccaa cgcgcaagcc accaatgcgg ttattccacc
    27961 ggccagaatg cctgataact gatttagcga tcagtttcgg tgatccacag ctggccacct
    28021 tccatccacg ccagccattg accatccggc gagaagacca cggcgtccgc agaaggtgga
    28081 ttggcgtggt tttcggttaa atactcaacc tcgccgcttt gcgcatgggc acaaacaatt
    28141 cgattatcca gcacaaagcc caaccattct cctgacggat gccagttaaa tgcagactga
    28201 atatccgttt tgttatggtt aacttgcgcg gttcgccgcc ctgtggcgag ataagccaca
    28261 gttgcacaat gccgttatca tcacgcatta aaaacgcgat ttgcgtaccc tgcggattac
    28321 agcgcaccca gtggcgcggg acgttgacta accccggata agcccgatga tgggtaaagg
    28381 ttaaacgtcg ctgcacgacg ccacgcggtg gcgcgggtag ggttgtttcc gttccactta
    28441 acggcgcatc acctgccgat ttccagctag cttcatcttg cggtaactca acgataaata
    28501 gctccggcac tttctcgccc tttggcgaaa gtgtgtcgcc aataaacgcc agcgcgtgat
    28561 ttcctaccca tccttcttca taagcacgat tgatttcatc actgccaggc tgcggcgtgg
    28621 gcgtggtttt actcaccagc acacaccagt ggctaccgct gtattcacgc ggatgttgct
    28681 tttgtacgtt gaccgggcca aacggtgcag caaccccgac gtttcgcaaa tccagcgccg
    28741 gatcgagttg atgcattaca tggtcgttat aggtaaagct ccccctttca ccgttcgggc
    28801 taaagacatg cacatggctg ccaccgcgca gcgctcctgg cgtatacggc gcggtaatat
    28861 ccattgcatc gagattgctc accttgccgc cttcagcaat gactccgcgt cgatgatgga
    28921 agtcgtaatg ccatgtttca tcaggatttt cagggccgtg aataaagaca tatttctctg
    28981 acttcggatg aacggtcacc acgccgacgt gtgcgccctg tgacgcgcga tagataacct
    29041 cgacctcgcc tgtatggata ttcacacgct caatggtctc gccggtaaac gacgccccag
    29101 aaggacgcac gtcaaatacc agccactggc tgtcgggcgt ccaggtattg gtattggtat
    29161 tggtgagcag gtgattacgg ggagcaaagg ttatctgttt catggcgcgg tcctgataca
    29221 aaacggttcc cgccatcata cttcacaagg acttcacttt cagacaatag tcttttgcca
    29281 tcgtgatgaa tgtgagtgaa aagatggaac atttcgacgt ggcgattatt ggcctcggcc
    29341 cggcagggtc ggcgttggca cgaaagttag ccggcaaaat gcaggtgatc gcgctggata
    29401 aaaagcacca gtgcggtact gaaggtttca gcaaaccttg cggcggtctg ctggcaccgg
    29461 acgcgcagcg atcttttatt cgcgatggac tgacgcttcc tgtcgatgtg atcgccaatc
    29521 cacagatttt cagcgtcaaa actgtcgacg tcgccgcatc gctcacgcgt aactaccagc
    29581 gaagctatat caatattaat cgccatgctt tcgacttgtg gatgaaatcg ctgatccccg
    29641 ccagcgttga ggtttatcac gatagcctgt gccgaaaaat ctggcgtgag gatgataaat
    29701 ggcatgtcat ttttcgtgca gacggctggg agcaacatat taccgcccgc tatctggtcg
    29761 gtgccgatgg cgcaaactcg atggtgcggc gacatctcta cccggatcat caaatccgta
    29821 aatatgtcgc tatccagcag tggttcgcag agaaacatcc ggtgccgttc tactcctgca
    29881 tctttgataa tgcgataact gactgttact catggagtat cagcaaagat ggttatttta
    29941 tctttggcgg tgcctatcca atgaaagacg gtcagacgcg tttcacgacg ctgaaagaga
    30001 aaatgagcgc ctttcagttc cagtttggca aagcggtgaa aagtgagaaa tgcacggtgt
    30061 tgtttccctc acgctggcag gattttgtct gtggtaagga caacgccttt ctgattggtg
    30121 aagcggcggg atttatcagc gccagctcgc tggaggggat tagctatgcg ctggatagcg
    30181 cagagattct gcgtgcggtg ttcctgaaac agccggagaa gagcaacgcc gcttactggc
    30241 gcgccacaca caaactgcgt ttaaaactct tcggcaagat agtaaaaagc cgatgcctga
    30301 ccgcaccggc tttaagaaag tggattatgc gcagtggtgt ggcgcatatt ccacagttga
    30361 aagattatcc aacgcgcttc acatcaccca ccagcaggat gtaagagagc gcgccaatca
    30421 acgcgacggc ggagatataa accagtgcag gcgcgaaacc gtaaccctgc gccaggtacc
    30481 ccaccaccag cggaacggtg atgccgccca gaccaccggc gaagttaaac acgccgccgg
    30541 ttaaaccaat caggcgcatc ggtgccagag aagagaccag cgaccaggta atcgaagcaa
    30601 aaccgttgcc gaagaatgcc agcgccatca ggcacataat catcatcgga tcgttggtgt
    30661 agttagcgcc cataatgcag gtggagatca gcaagccgca gataatcggc gttttacgcg
    30721 caaagcccag tgaaaagccc ttacgtacca gcagatccgc gacccagcca gagagcagga
    30781 cgccgacaaa cgccgcgagg aatggcaccg tggtcataaa gcctgctttc agcgccgtga
    30841 ttcctttttc ctgggttaaa tagttcggga accaggttaa gaaaaaccac agtgcagaag
    30901 ccaccgcaaa ttgcccaaga taaacgccga tcagtttacg atggaacacc agtttccagt
    30961 ctttggctgt taacggctga cgcgcctctt tcttcaccgg cgcatcacca tccaccagac
    31021 cgccgccatc acgaatgtaa tccagttcag ctttgctgat acctttggtc aggcgcggcg
    31081 gctgataaac cttaaaccaa atcagcgacc agataatgcc gataccacca gtgacaatga
    31141 acacccagtg ccagctcaac atctcctgaa tccagatcag cagcggagtc aaaaacgcca
    31201 gaccgacaaa ctgaccagac gtataaaaac caacggcgga agcgcgttca tgttccggga
    31261 accagctggt caccatccgg ttattggtcg ggaaagcggg cgcttcgaaa ataccggtta
    31321 tcgcacgcag accaattaat gacattaatc ccgtggcaaa gccttggaat aaagtcacca
    31381 ctgaccaacc aaatatcgca ataaaataag tcacgcgaga acctacgcga tctaaaaacc
    31441 aaccgccggg gatctggcat agcgtataaa gccaggcgaa ggccgaaaat acatagccca
    31501 tttccgcttt cgtaatgccg aactcttcct gaatatgggc ggaagccacg gccaagttag
    31561 cgcggtcaac ataacaaatg accaccgtaa taaagatcat caccagcgtc agataacgcc
    31621 gacgccccgg ctttgctgca ttaacgggaa tatccatagc gagctttctc cagattttgg
    31681 gcatagcgaa gccgctcacc atgccctgta atttacagag ggttatattt ttgtattgct
    31741 gttttagtgc ccgatgaggg gcttacgtgg caggaattac cactctgcta cgctgttatc
    31801 ttcatgacgc cagagcggat tacgccagtc cggggcattt ttactgaact caataacttt
    31861 agcttcgtcg atttccacac ctaagcccgg tttcgttaac ggtttaaaga agccgccaac
    31921 catgctgaag tcttctttgt ttttcacaaa gtcgagtaac tccgcgcctt tgttgtaatg
    31981 aatgcccata ctttgttact gaagtaccgc gttataggaa acaaagtcga tatgcaggca
    32041 agccgccagt gcaatcggtc cgaacggaca gtgcggcgca agggtcacat cataggcttc
    32101 tgccattcca gcaattttgt agcattcggt aataccgcct gcatgggaga gatccggttg
    32161 cagaatcgaa ataccgcctg cctccagcac gcgtttaaaa tcgaaacgtg agaacatgcg
    32221 ctcacccgcc gccagtggaa tatgcgtttg tgccgccaat ttcgggtagt attcggcttg
    32281 ttccgccagc accggctctt caataaacag cgggcgatac ggctccagtt ctttaatcag
    32341 cactttcgcc attggcgcgc tgacgcgacc gtggaaatca agaccaaact caatctgatt
    32401 gccaaaagct tcacgaattt gtgccacggt gttgactgcc gcatctaccg cgcgggagtt
    32461 atcaattagc cccagttctt cacaaccgtt cagtttgaag gtatcgaagc cgatttcgcg
    32521 cagggtttta atgccgtcga taacatccgc cggacgatcg ccgccgaccc aactgtaggc
    32581 tttaattttg tcgcgaacca ggccgcccat cagttgccag actggcgcat tcagcacttt
    32641 gcctttgata tcccataacg cctggtcgat cccggcgatg gcgctcatca ggattggacc
    32701 gccacgataa aatccggcgc gatacatcac ttgccataag tcgttgatgc gcgaaggatc
    32761 ctgaccaatc aaatagtcac ccagctcgtg aactgccgct tccaccgtac gggcgcggcc
    32821 ttcaatcacg ggctcgcccc aaccgaccac gccttcatcg gtttcaattt tcaggaacat
    32881 ccagcgggga ggtaaacgat acgtggtaat tttggtgatt ttcattgcac tgcctctcga
    32941 tacgccttaa caaatgctgc tgcctgctgc gcggtgcgtt ctacggactg cccggcgcga
    33001 taaagatcgc tgcctaagcc cgccccaaca caacctgcgt ctatccactg cgccaggttt
    33061 tctggcgtca cgccgccaac ggcaaagact gcgatgtccg gtggtaatac cgcttttaac
    33121 gctttgatgt attgcggacc aaaagccgat gacggaaata ttttcagcgc ctgcgcgccc
    33181 gcttcgagcg cggtaaaggc ttcggtcgcc gtcgcgcagc cggggcaaac ggtcatgccg
    33241 tagcccaccg cgcggcggat cacttcacta tggatattgg gcgtaacgat gagctgacag
    33301 cccatcctgg cgagcgcatc gacctgttca ggtttcagta ccgtacctgc gccaatcaac
    33361 gccttgtcgc catacgcatc aacgatggcg ggaatgctct gctcccattg tggggaattc
    33421 agcgggattt caaccgcgtc gaacccggcg tcaatcaccg cgccaacatg cgccagcgcc
    33481 tcgtcgggcg taatgccgcg caaaatggcg atcagcggga gtttagtttg ccactgcatg
    33541 agcgatgctc cttataccag cctgaaatgc cgtgtcgccc gccaccgccg ccacgtcgca
    33601 acccatcgcc tgaaaggctt gctggtagcg cgcggtcagc gatgttccgg cgacaagggt
    33661 gatggcgtgt tgatgggtca catagtcgcg catactggca acctctgcgc caatcaacaa
    33721 accagagaga aattcgctga cctgttcgcg gggaagtgtt cccagcacat gcgaggcgcg
    33781 aacttcaaaa agctgcggca atatggcggg cgcattaagg ccgcgctcaa ggccagccgc
    33841 gaaggcatcg gcagagtttt cctgcggcgg caaacctgcg ccaatcaatg agtgatttaa
    33901 cagtaaatga tgtaattcac cggtcatcac ggtgcgaaaa tcgttgattt gctggctatc
    33961 ggcctgcacc catttgcaat gcgtaccggg cataacataa agagaggaag gagccagagc
    34021 tcgcgcgccg atcaattgtg tttcttcgcc gcgcatcaca ttgtggttat cgtcatgaga
    34081 gacgcataat ccgggaataa tccagatatt gtcgccaaca gacgttaatt gttcgccaat
    34141 agacgaaaaa cgggcaggaa cagataaata cggagcaact ttccagccga cattgctgcc
    34201 gaccattcct gccatcacca ctggcgtatt ttcttcacgc cagtcggtcg tgacttctgc
    34261 taacaccaca gccggagatt ttccgttcag gcgcgtgacg cctgcttctg attgcctgct
    34321 ctccaggcag tggtcgccct gataaagcca ggcgcgcaga ttggtcgatc cccagtcaat
    34381 tgcgatgtag cgagctgtca tgtgatttcc tttaaccttc gtgtcgagct ggcaatcatg
    34441 gtaagcgccg cctgctctgc cgcatcgccg tcctgatgcc gtatcgcatc gaacagcgcc
    34501 ttatgttcct ggagcgtttg cggcatgttg gcctcatcgc ccatccaggt tcgttcaaaa
    34561 accgcccgct gcagcgaact gatcgcaatg ctaagttgct gtaacaccgg gttatgcacc
    34621 gactgcagca ccgcctcgtg gtagcgaata tccgcttcgt taaacgcttc gcggtcctga
    34681 ttgttggcaa tcatctcgtt cagcgccgat tcaatctgcg ccagatcgct ggaggtcgcg
    34741 cgctctgctg cccaacgggc aatcgccggt tccaccagat ttcgcacttc gctcatggca
    34801 ctgataagcc gtgggtcgta gtcgttttcc agcacccatt gcagtacgtc agtgtcgagg
    34861 taattccact ggttacgcgg tgccacaaac gccccgcgat aacgtttcat ttcaatcagc
    34921 cgcttcgcca tcagcgaacg gaacacctca cggatgatgt tgcgcgaggt tgcaaactct
    34981 tcacagagct ccgcctcagc cggaagtggc gagccaggta cgtatttgcc gtgaacgatc
    35041 tgtttaccca gcgtaatgac aatgcgatcg gttttattga gagtcatgga gagtccttgt
    35101 gctctgtatg ttcttctcta ctttaccccg atcgatgcat aacgcggcaa ctttgtagta
    35161 ccagcgtgat gacgttcgcg tttgccgcgc gcgtaatgta gtacaaactt atattgttat
    35221 gctacaattt agatcaaaaa aagaacaata cataaaaaaa tgacatgcgt cgggcagaaa
    35281 tctgaaaagg gatatcaggc gctaaacagg agggaaagaa gagtatgctt tcaacggctt
    35341 agctactcgt ttaaaggatt aatcatgaag ttgaatttta agggattttt taaggctgcc
    35401 ggtttattcc cactggcgct gatgctttca ggctgtatct cgtatgctct ggtttcccat
    35461 accgcaaagg gtagttcagg aaagtatcaa tcgcagtcag acaccatcac tgggctatcg
    35521 caggcaaaag atagtaatgg aacaaaaggc tatgtttttg taggggaatc gctggattac
    35581 cttatcactg atggtgccga tgacatcgtt aagatgctca atgatccagc acttaaccgg
    35641 cacaatattc aggttgccga tgacgcaaga tttgttttaa atgcggggaa aaagaaattt
    35701 accggcacaa tatcgcttta ctactactgg aataacgaag aagaaaaggc actggcaacg
    35761 cattatggtt ttgcctgtgg tgttcaacac tgtaccaggt cactggaaaa cctaaaaggc
    35821 acaatccatg agaaaaataa aaacatggat tactcaaagg tgatggcgtt ctaccatcca
    35881 tttaaagtgc gattttatga atactattca cccagaggca ttccggatgg tgtttccgca
    35941 gcattactgc cagtgactgt tacactggac atcattactg caccgctgca atttctggtt
    36001 gtatatgcag taaaccaata atcagtaagc gggcaaacgc gtttatgctg tttgcccgct
    36061 tactgattaa ttcagcacat acttctcaat agcaaacgcc acgccatctt caaggttgga
    36121 ttttgtgaca aagttcgcca cttctttcac cgaaggaata gcgttatcca tcgccacacc
    36181 gacgcctgca tattcaatca ttgcgatgtc gttttcctga tcgccaatcg ccatgatttc
    36241 ttccggttta atacctaaca cgtcggccag tgatttcaca cccgtacctt tgttaacgcg
    36301 tttatcgagg atttcgagga agtacggcgc acttttcagc acggtatatt tctctttcac
    36361 ttcctgcgga atacgcgcga tagcctggtc gaggatggcg ggttcatcaa tcatcatcac
    36421 tttcaggaac tgggtattgg ggtccatttt ctccgcttcg cagaacacca gcggaattgt
    36481 ggcaacgaag gattcatgca ccgtgtagta gctgatatcg cggttagcgg tgtacagcgt
    36541 ggtgcggtcc agggcgtgga aatgagaacc gacttcgcga gagagttttt ccaggaaacg
    36601 atagtcgtca tagctgagag cggtttgcgc cacggtgcta ccatcagcgg ccttctgtac
    36661 cagcgcgccg ttataagtaa tgcagtagtc gcccggctgt tccatatgca gctctttcag
    36721 gtagttgtgc acacctgcat acggacgacc cgtcgttagc acgacattca cgccacgggc
    36781 gcgagctgcg gcaatcgcat ttttaacggc gggtgaaatg gtgtgatcgg gcagcagaag
    36841 ggtgccatcc atatcgatag caatgagttt gatagccatg agttcccaag gtagattagt
    36901 tcctgaccca tgctaacgcg attccgctca aaaatcagta caacacccga gggaaaaggg
    36961 gatgcaacgc gggttgcatg ctccccattt gcttagcgga agagtttccc tttcagcagt
    37021 tccatgcctg cggaaagcag atcgttattg gcttgtgctg acacttcacc ttgcggtgag
    37081 agcgcatcaa taatcttcgg caattgctct gccagtaaac tggaagccgt actggtatcc
    37141 acgccaagtt tttgcccgag atcggacacc gcatttgtgc cgagcgccga ctccagttgc
    37201 tcgccgctaa ctgattgatt tctctgttga ttactcaacc aggttgagag aatggcccct
    37261 aagccgccac tttgcagttt ttccagcagc acctgaatgc caccctgctc ctcaacccaa
    37321 cttaaaatcg cctgatattt ccccgcatcg cctttcagaa aggcaccgac aacttcatca
    37381 aaaagcccca tgataatcac ctgtaaagcg ttacgtgttg acccaaaaag tatagatctg
    37441 cgggatgata attgcggatt gcagaaataa aaagggcgga gatgatctcc gcccttttct
    37501 tatagcttct tgccggatgc ggcgtgaacg ccttatccgg cctacaaaat catgcaaaat
    37561 caatatattg caggagtctc gtaggactaa taagcgtagc gcatcaggca cgctcgcatg
    37621 gttaacgcca ttaaatatca atattcgccg ctttcagggc gttctcttca ataaacgcac
    37681 gacgcggttc tacggcgtcg cccatcagcg tggtgaacaa ctggtcagcg gcaatcgcat
    37741 ccttaacggt aacgcgcagc atacgacggc tttccgggtc catggtggtt tcccacagct
    37801 gttccgggtt catctcgccc agacctttat aacgctggat ggagaggcca cgacgggact
    37861 ctttcaccag ccagtccagc gcctgctcga agctggctac cggctgacga cgctcgccac
    37921 gttcgataaa cgcatcttct tccagcaagc cacgcagttt ctcacccagc gtgcagatac
    37981 gacgatattc gccaccggtg ataaactcgt gatccagcgg atagtcagta tccacaccgt
    38041 gggtacgcac gcgaacaatc ggctcgaaca ggttttgttc ggcattggtg tggacatcaa
    38101 acttccactg gctgccgtgc tgttctttgt cgttcagttc gctgaccagc gcgttcaccc
    38161 agcgggtaac ggtctgctca tcagaaaggt cagcttccgt caacgtcggc tgatagataa
    38221 gctctttcag cattgctttc ggataacgac gctccatgcg gttgatcatt ttctgcgtcg
    38281 cgttgtactc agataccagt ttctctaacg cttcgccagc caatgccggt gcactggcgt
    38341 tggtgtgcag cgttgcgccg tccagcgcga tagagatctg gtactgatcc atcgcttcgt
    38401 cgtctttaat gtactgttcc tgcttgcctt tcttcacttt gtacagcggc ggctgagcga
    38461 tgtagacgtg accgcgttca acgatttccg gcatctgacg atagaagaag gtcaacagca
    38521 gcgtacgaat gtgcgagccg tcgacgtccg catcggtcat gatgatgatg ctgtgataac
    38581 gcagtttgtc cgggttgtac tcgtcacgac cgataccaca gccaagcgcg gtgataagcg
    38641 tcgccacttc ctgagaagag agcatcttat cgaagcgcgc tttctcgacg ttaaggattt
    38701 tacccttcag cggcagaatc gcctggttct tgcggttacg cccctgcttc gcagagccgc
    38761 ccgcggagtc cccttccacc aggtacagtt cggaaagcgc cggatcgcgt tcctggcagt
    38821 ctgccagttt gcccggcagg ccagctaagt cgagcgcacc tttacggcgg gtcatttcac
    38881 gcgcgcgacg cgccgcttca cgggcacggg cagcatcgat aattttgcca accacgattt
    38941 tcgcatcagt tgggttttcc agcaggtatt ccgccagcag ttcgttcatc tgctgttcaa
    39001 ccgccgattt cacctcagaa gaaaccagtt tgtctttggt ctgggaggag aatttcgggt
    39061 ccggcacttt tacggaaacg accgcaatca ggccttcacg cgcatcgtca ccggtggcgc
    39121 tgactttggc ttttttgctg tagccttctt tgtccatgta ggcattcagg gtacgggtca
    39181 tcgccgcacg gaagcctgcc aggtgagtac cgccgtcacg ctgcggaatg ttgttggtaa
    39241 agcagtagat gttttcctgg aagccatcgt tccactgcag cgccacttca acgccaatac
    39301 cgtctttttc ggtggagaag tagaagatat ttgggtggat cggcgttttg ttcttgttca
    39361 gatattcaac gaacgctttg atgccgcctt catagtggaa gtggtcttct ttgccgtcac
    39421 gcttgtcgcg cagacgaatg gaaacgccgg agttgaggaa cgacaactca cgcagacgtt
    39481 tcgccagaat ttcatattcg aactcggtca cattggtgaa ggtttcgagg ctgggccaga
    39541 aacgcaccat ggtgccggtt ttttcagtct cgccggtaac cgccagcgga gcctgcggta
    39601 caccgtgttc gtagatctga cggtgaattt taccctcgcg ctgaataacc agctccagtt
    39661 tttgcgacag ggcgtttact accgaaacac caacgccgtg cagaccgccg gacactttat
    39721 aggagttatc gtcaaattta ccgcctgcgt gcagaacggt catgatcact tccgccgccg
    39781 atacgccctc ttccgggtga ataccggtcg gaatgccgcg cccgtcatcc tgtacagaga
    39841 cagagttatc ggcgtgaatg gtgacgataa tttctttaca gtgacccgcg agcgcttcgt
    39901 cgatagcgtt atctaccacc tcgaatacca tgtggtgcag accggtgccg tcatccgtgt
    39961 cgccgatata catacccggg cgcttacgca ccgcatccag ccctttcagg actttgatac
    40021 tggaggagtc ataagaattc gacatcaacg tttctcgctc atttatactt gggttaatct
    40081 gttattttac ccttttccac ggtaaacatc ttcgaatttt cgtccgacat gtctataacg
    40141 tgttcagcac tgatcgcgct gacaaagacc tgtgattgcg tcgcttttaa gcggctggca
    40201 agcaacccgc gacgctcatc atcaagctca gaggcaaaat catctatcag gtagagacac
    40261 cgccgcccgc tttcacgagt gaggaactct ccttgcgcca gacgtaaggc gcacatcaac
    40321 agcttaagct gcccacgcga taaggtatct tccaccggcg caccgtcggc gcgaatgcgt
    40381 aagtccgctt tatgcgggcc atgcgcggtg taggttagct ggcgatcgcg ttcaaaatta
    40441 cgttccagca cctcagcgta ctctgtctct ttctcccagc cgcgctggaa agagaaagtc
    40501 agagaaaact cagggagaaa ttgcttacag gtatcatcca tatcggccgc gataccggcg
    40561 ctatactccg cgcgccaggt gctgatttgc tccgccagcg ggatcagttc tttatcccac
    40621 gggcgtagct gttcgtaacg agtcacctgg cgcagcgccg cattgcgctg cttgagcaat
    40681 cgcttgagat tgctccaggc ggtgaaaaat ccaggttcgt tgtgaaagca tccccagtcg
    40741 aggaatgctc ttctgtattt ggggccgccg ttgagtaaag taaacccttc tggcgttatc
    40801 aactgcatcg gcatcaggtg cgccagttcc gcgactttat gcccgtccgt accgtcaata
    40861 cggactttgc tgtcgccctg tttgtctttg gttaagccaa tcgccgtctc gcgctcttcg
    40921 ccctgtaatc gcccgtggag aacaaatgcc tcctgctcat ggcgaatgac gcgaccaatc
    40981 tgcaaactgc gaaacgcccg accatggccg agcgtataga tggcttccag cacgctggtt
    41041 ttgccactgc cgttggcacc taccagaaag ttaaagccgg gagataaagc gagatccgcg
    41101 gtttcaatat tgcggaaatc gcggatcaac aagcgggtga gggacattac agtctcattg
    41161 gcatgacaac ataggcagcc gactgtgatg cggcatcttc aatctgcacg ctggaaaccg
    41221 aatcggtcag catcatgcgg acgttttcgc atttcagcgc gttcagaaca tccagcacat
    41281 agctgacgtt gaagccgatt tccatctccg caccgctata ggtaacgtcg aggatctctt
    41341 ccgcttcttc ctgttccggg ttgttggcag taattttcag ctggttttcg ctgacataca
    41401 ggcgcacacc gcggaatttc tcgttagaga gaatcgccgc gcgagcaaac gcctgcttga
    41461 gcagatcgca gcctgcttcc agatgtttgt ccgggttctt cggcagaacg cgacggtaat
    41521 ccgggaagcg accatccacc agtttcgagg tgaaaataaa gtcgccaacg tgggcacgaa
    41581 tgttattgct gccaatctgc acacgcagca gattgtcgcc gccgtcgagc atacgcatca
    41641 gttcaatcac gcctttacgc ggtacgatca ccgaatggct tggcaaagat tgaccaattg
    41701 gcattgaaca gaccgccaga cggtggccgt cggttgccac ggtgcgcagt tcttcacctt
    41761 cggtttcaaa cagcataccg tttaagtaat agcgaacatc ctgatgcgcc atcgaaaact
    41821 gggttgcttc aatcagacgc ttcatcgtcg cctgcggcag ggtaaattcg acttcgctct
    41881 gccagtcatc gaggttcggg aaatccgccg ctggcagggt agacagcgaa aaacggctac
    41941 gcccggagcg caccagcatc cgttcacctt ccagctgcac ggcaatttcc gcgccttcag
    42001 gcagaccacg gcagatatca aagaatttgc gcgccggaac ggtcgtcgct cctggctcgt
    42061 gtggctgaac cagcgcaacg cgtgccacca tttccatctc gagatcagta ccggtcagcg
    42121 acaacgtacc gtcagcaacc tgtaacagca gattaccgag aatcggtagc gtaggacgac
    42181 cacctaacgg accgctcacc tgttgtagcg gttttaataa atgctcacgt tctacggtaa
    42241 atttcatagg tttacgatga caatgttctg attaaatttg aaaaatcttc tttgatatcg
    42301 tggctctctt cacgcaactg ctcgatctta cggcaggcat gaagcaccgt cgtgtggtca
    42361 cgcccaccaa acgcatcgcc aatctctggc agactgtggt tagtcagctc tttcgctagc
    42421 gccatcgcca tctggcgcgg acgcgccacc gagcgggatc gacgcttgga aaggagatcc
    42481 gcgactttga tcttgtagta ctccgccacc gtcttctgaa tattgtcgat ggtgaccagt
    42541 ttttcctgca atgccagcaa gtcgcgcagc gcctcacgca cgaagtcgat ggtgatcgcc
    42601 cgtccggtaa agttggcgtt agcaataacg cggttcagcg ccccttccag ctcacgtacg
    42661 ttagatcgta gacgcttggc gataaagaac gccacttcac ccggcaaacg aatgtcattt
    42721 tcgtcggcct ttttcatcag gatcgccaca cgggtttcca gctctggcgg ttcgatcgcc
    42781 acagtcagtc cccaaccgaa gcgggatttc aaacgatcct caacgccgtt gatctctttc
    42841 ggatagcgat ccgaggtgag aatgatctgt tgattacctt ccagcagggc gttgaaggtg
    42901 tggaaaaact cttcctgaga tcgttcttta ttagcaaaaa actgaatatc gtcgatcagc
    42961 agtgcatcta cggaacggta gtagcgttta aactcttcga tcgcgttgtt ttgcagggct
    43021 ttaaccatgt cctgaacaaa gcgctcggag tgcatataaa ccactttggc attcggcttg
    43081 cgcgccataa tgccgttacc caccgcatgc agcaggtgag ttttacccag acccgtgccg
    43141 ccataaagga acaacgggtt ataggcaccg ccagggttat ccgccacctg gcaagccgcc
    43201 gcgcgcgcca gttggttaga tttaccttca acgaagttat caaacgtgtg tttgacgttt
    43261 acgttagaac gataggtcgg ttctgccggg gccgggacgt tatcccaacc tgagcgcgta
    43321 gaaggcgcag cacgttgcgg ctgcgtttgc gccacctgtg caggggccgc gacgttgctc
    43381 gtcactgccg cttgtggcgt ttgcgtcacc ggtttggtgc cgacttcaaa acgcagctgt
    43441 ggggcatccg ctccgcagaa actggttagc agtccattga tattattaag gtacttgtcc
    43501 cgtacccaat cgaggacaaa acggtttggc gcgtacaggg ccagcgtgtt atcgctcagt
    43561 tccgcctgca atgggcgtat ccacatactg aattctgtgg ctggtaactc atcctgcaat
    43621 cgggcaagac actgctgcca aagcgaaagt gacacggcgg actccactcg aacaaaagtc
    43681 gataatgact aaggctgaaa cattcatgat tgttgacgta cgtcgaaaag accctgcttg
    43741 agggtgacgc acgaaccgct gtctgcggtt atatgcccga tcaagatcct gcaaaacgat
    43801 cgggaccgcg gatcatagcc taaactgcgc aagagatctt ctgtttctca cagattttcc
    43861 ctatttatcc acaggacttt ccagaactcg ctaagtgtaa acgatcctgc cgcgaggcgg
    43921 gcacgattta cgccgcatat tggaaaattt aatgaccata gacaaaaatt ggcttaatcg
    43981 atctaataaa gatccaggac gatccttgcg ctttacccat cagcccgtat aatcctccac
    44041 ccggcgcgcc atgctggttt ccactggtgt gaggtcgtac attttccctg cgaaaaggtg
    44101 cggaaaagcg cggtaaataa ggaaagagaa ttgactccgg tgtgtacaat tattacaatc
    44161 cggcctcttt aatcacccat ggcttcggtg tccatcgttt catttttcgg cggatatcca
    44221 ataaagccat tgaatttatt caagtttagg tagaaatcgc catgaaacgc acttttcaac
    44281 cgtctgtact gaagcgcaac cgttctcacg gcttccgtgc tcgtatggct actaaaaatg
    44341 gtcgtcaggt tctggcacgt cgtcgtgcta aaggccgcgc tcgtctgacc gtttctaagt
    44401 aataaagcta acccctgagt ggttaagctc gcatttccca gggagttacg cttgttaact
    44461 cccagtcaat tcacattcgt cttccagcag ccacaacggg ctggcacgcc gcaaattacc
    44521 attctcggcc gcctgaattc gctggggcat ccccgtatcg gtcttacagt cgccaagaaa
    44581 aacgttcgac gcgcccatga acgcaatcgg attaaacgcc tgacgcgtga aagcttccgt
    44641 ctgcgccaac atgaactccc ggctatggat ttcgtggtgg tggcgaaaaa aggggttgcc
    44701 gacctcgata accgtgctct ctcggaagcg ttggaaaaat tatggcgccg ccactgtcgc
    44761 ctggctcgcg ggtcctgata gccctcattc gggtctatca acgcctgatt agtccgctac
    44821 tcgggccgca ttgtcgtttc actccaacct gttcaagcta cggaattgag gcattgcgca
    44881 ggtttggagt gataaaaggc agttggttga cggtgaaacg cgtattaaaa tgccaccctt
    44941 tacaccctgg tggtgacgat cccgtcccgc ccggaccatt tgataccaga gaacactaac
    45001 gatggattcg caacgcaatc ttttagtcat cgctttgctg ttcgtgtctt tcatgatctg
    45061 gcaagcctgg gagcaggata aaaacccgca acctcaggcc caacagacca cgcagacaac
    45121 gaccaccgca gcgggtagcg ccgccgacca gggcgtaccg gccagtggcc aggggaaact
    45181 gatctcggtt aagaccgacg tgcttgatct gaccatcaac acccgtggtg gtgatgttga
    45241 gcaagctctg ctgcctgctt acccgaaaga gctgaactct acccagccgt tccagctgtt
    45301 ggaaacttca ccgcagttta tttatcaggc acagagcggt ctgaccggtc gtgatggccc
    45361 ggataacccg gctaacggcc cgcgtccgct gtataacgtt gaaaaagacg cttatgtgct
    45421 ggctgaaggt caaaacgaac tgcaggtgcc gatgacgtat accgacgcgg caggcaacac
    45481 gtttaccaaa acgtttgtcc tgaaacgtgg tgattacgct gtcaacgtca actacaacgt
    45541 gcagaacgct ggcgagaaac cgctggaaat ctcctcgttt ggtcagttga agcaatccat
    45601 cactctgcca ccgcatctcg ataccggaag cagcaacttc gcactgcaca ccttccgtgg
    45661 cgcggcgtac tccacgcctg acgagaagta tgagaaatac aagttcgata ccattgccga
    45721 taacgaaaac ctgaacatct cttcgaaagg tggttgggtg gcgatgctgc aacagtattt
    45781 cgcgacggcg tggatcccgc ataacgacgg taccaacaac ttctataccg ctaatctggg
    45841 taacggcatc gtcgctatcg gctataaatc tcagccggta ctggttcagc ctggtcagac
    45901 tggcgcgatg aacagcaccc tgtgggttgg cccggaaatc caggacaaaa tggcagctgt
    45961 tgctccgcac ctggatctga ccgttgatta cggttggttg tggttcatct ctcagccgct
    46021 gttcaaactg ctgaaatgga tccatagctt tgtgggtaac tggggcttct ccattatcat
    46081 catcaccttt atcgttcgtg gcatcatgta cccgctgacc aaagcgcagt acacctccat
    46141 ggcgaagatg cgtatgctgc agccgaagat tcaggcaatg cgtgagcgtc tgggcgatga
    46201 caaacagcgt atcagtcagg aaatgatggc gctgtacaaa gctgagaagg ttaacccgct
    46261 gggcggctgc ttcccgctgc tgatccagat gccaatcttc ctggcgttgt actacatgct
    46321 gatgggttcc gttgaactgc gtcaggcacc gtttgcactg tggatccacg acctgtcggc
    46381 acaggacccg tactacatcc tgccgatcct gatgggcgta acgatgttct tcattcagaa
    46441 gatgtcgccg accactgtga ccgacccgat gcagcagaag atcatgacct ttatgccggt
    46501 catcttcacc gtgttcttcc tgtggttccc gtcaggtctg gtactgtact atatcgtcag
    46561 caacctggta accattattc agcagcagct gatttaccgt ggtctggaaa aacgtggcct
    46621 gcatagccgc gagaagaaaa aatcctgatt cggtgagttt tcgctaaaat aagggcggtc
    46681 agttgaccgc cttttttctt ttcgtagggc ggataagcac cgcgcatccg ccacacaaag
    46741 caacaggaac atcatgagcg ataatgacac tatcgtagcc caggccacgc ctccgggacg
    46801 tggcggcgtt ggcatcctgc gcatctccgg cttcaaagcc cgtgaagttg ccgaaaccgt
    46861 gctgggtaaa ctgcctaagc cgcgctacgc cgattatctt ccgtttaaag acgccgacgg
    46921 cagcgtgctc gatcagggga ttgcgctatg gttccctggc ccgaactcgt tcaccggcga
    46981 agatgtgctg gaactgcaag gtcatggcgg tccggtgatc ctcgacctgc tgttaaaacg
    47041 cattctgacc attcccggcc tgcggattgc tcgccctggt gagttttccg aacgcgcgtt
    47101 tcttaacgat aaacttgact tagcccaggc cgaggcgatt gccgatctta tcgacgccag
    47161 ttcggaacag gcggcccgtt cggcacttaa ctcgctgcaa ggcgcattct ccgcacgggt
    47221 taatcatctg gtagaagccc tcacccactt gcgcatttac gtcgaagcgg caattgattt
    47281 ccctgatgaa gagatcgatt tcctctccga cggcaaaatt gaagcccagc ttaatgacgt
    47341 gatggccgat ctcgatgcag tgcgtgctga agcacgtcag ggtagtttgt tgcgcgaagg
    47401 gatgaaagta gtgattgccg gacgtcctaa tgccggtaaa tcgagcctgt taaacgcgct
    47461 ggcgggccgt gaagcggcaa tcgtaaccga tatcgccgga accacgcgtg acgtgctgcg
    47521 tgagcatatc cacattgacg gaatgccgct gcatatcatc gacactgccg ggctacgtga
    47581 ggccagtgac gaagtggaac gtattggtat cgagcgcgcg tggcaggaaa ttgaacaggc
    47641 cgaccgcgtg ctgtttatgg tcgatggcac cacgacagac gccgttgatc cggcagagat
    47701 ctggccggaa tttatcgccc gtctgcccgc gaaactgccg atcaccgtgg tacgcaataa
    47761 agccgatatc accggcgaaa cgctgggaat gagtgaagtg aacggtcacg cgttaattcg
    47821 tctctcggca aggactggtg aaggcgtgga cgtgctgcgt aaccatctca aacagagcat
    47881 gggctttgac accaatatgg aaggcggctt cctggcgcgc cgtcgccact tacaggcgct
    47941 ggaacaggca gcggagcatt tgcagcaagg taaagcgcaa ctgttgggag cctgggcagg
    48001 tgaactgctg gcggaagagt tgcgcctggc acagcagaac ttaagcgaaa tcaccgggga
    48061 atttacttca gacgacctgc tggggcggat tttctccagc ttctgtattg gtaagtaacc
    48121 gcgcttacga agccgtattc tgactgtcag atgcggcttc gcttcattgt tagcactcct
    48181 gttatgcctc aacccttttt taaacattaa aattcttacg taatttataa tctttaaaaa
    48241 aagcatttaa tactgctccc cgaacgattg tgattcgatt cacatttaaa caatttcaga
    48301 atagacaaaa att
//
LOCUS       DAEEUE010000023        47334 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.023, whole genome shotgun sequence.
ACCESSION   DAEEUE010000023 DAEEUE010000000
VERSION     DAEEUE010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47334
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.023"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..480
                     /gene="sseA"
                     /locus_tag="KLK84_001366"
     CDS             <1..480
                     /gene="sseA"
                     /locus_tag="KLK84_001366"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HBE2008751.1"
                     /translation="LAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASHE
                     NTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIFF
                     GRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK"
     gene            complement(1301..2077)
                     /gene="sseB"
                     /locus_tag="KLK84_001367"
     CDS             complement(1301..2077)
                     /gene="sseB"
                     /locus_tag="KLK84_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HBE2008752.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2219..3502)
                     /gene="pepB"
                     /locus_tag="KLK84_001368"
     CDS             complement(2219..3502)
                     /gene="pepB"
                     /locus_tag="KLK84_001368"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HBE2008753.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3561..3761)
                     /gene="iscX"
                     /locus_tag="KLK84_001369"
     CDS             complement(3561..3761)
                     /gene="iscX"
                     /locus_tag="KLK84_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HBE2008754.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3773..4108)
                     /gene="fdx"
                     /locus_tag="KLK84_001370"
     CDS             complement(3773..4108)
                     /gene="fdx"
                     /locus_tag="KLK84_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HBE2008755.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4110..5960)
                     /gene="hscA"
                     /locus_tag="KLK84_001371"
     CDS             complement(4110..5960)
                     /gene="hscA"
                     /locus_tag="KLK84_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HBE2008756.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5977..6492)
                     /gene="hscB"
                     /locus_tag="KLK84_001372"
     CDS             complement(5977..6492)
                     /gene="hscB"
                     /locus_tag="KLK84_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HBE2008757.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6588..6911)
                     /gene="iscA"
                     /locus_tag="KLK84_001373"
     CDS             complement(6588..6911)
                     /gene="iscA"
                     /locus_tag="KLK84_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HBE2008758.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6928..7314)
                     /gene="iscU"
                     /locus_tag="KLK84_001374"
     CDS             complement(6928..7314)
                     /gene="iscU"
                     /locus_tag="KLK84_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HBE2008759.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7342..8556)
                     /gene="iscS"
                     /locus_tag="KLK84_001375"
     CDS             complement(7342..8556)
                     /gene="iscS"
                     /locus_tag="KLK84_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBE2008760.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8668..9156)
                     /gene="iscR"
                     /locus_tag="KLK84_001376"
     CDS             complement(8668..9156)
                     /gene="iscR"
                     /locus_tag="KLK84_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HBE2008761.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9426..10166)
                     /gene="trmJ"
                     /locus_tag="KLK84_001377"
     CDS             complement(9426..10166)
                     /gene="trmJ"
                     /locus_tag="KLK84_001377"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HBE2008762.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPLECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10285..11088
                     /gene="suhB"
                     /locus_tag="KLK84_001378"
     CDS             10285..11088
                     /gene="suhB"
                     /locus_tag="KLK84_001378"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HBE2008763.1"
                     /translation="MHPMLNIAVRAARKAGNLVAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11233..12087
                     /locus_tag="KLK84_001379"
     CDS             11233..12087
                     /locus_tag="KLK84_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBE2008764.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12278..13558
                     /gene="csiE"
                     /locus_tag="KLK84_001380"
     CDS             12278..13558
                     /gene="csiE"
                     /locus_tag="KLK84_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HBE2008765.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13550..14689)
                     /locus_tag="KLK84_001381"
     CDS             complement(13550..14689)
                     /locus_tag="KLK84_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HBE2008766.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14849..15739)
                     /gene="hcaR"
                     /locus_tag="KLK84_001382"
     CDS             complement(14849..15739)
                     /gene="hcaR"
                     /locus_tag="KLK84_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HBE2008767.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15875..17236
                     /locus_tag="KLK84_001383"
     CDS             15875..17236
                     /locus_tag="KLK84_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HBE2008768.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17233..17751
                     /locus_tag="KLK84_001384"
     CDS             17233..17751
                     /locus_tag="KLK84_001384"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HBE2008769.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGIAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17751..18071
                     /locus_tag="KLK84_001385"
     CDS             17751..18071
                     /locus_tag="KLK84_001385"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HBE2008770.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18068..18880
                     /gene="hcaB"
                     /locus_tag="KLK84_001386"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18068..18880
                     /gene="hcaB"
                     /locus_tag="KLK84_001386"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HBE2008771.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18890..20092
                     /locus_tag="KLK84_001387"
     CDS             18890..20092
                     /locus_tag="KLK84_001387"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HBE2008772.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            20189..20611
                     /locus_tag="KLK84_001388"
     CDS             20189..20611
                     /locus_tag="KLK84_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBE2008773.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20659..21531)
                     /locus_tag="KLK84_001389"
     CDS             complement(20659..21531)
                     /locus_tag="KLK84_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HBE2008774.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTTLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21543..22637)
                     /locus_tag="KLK84_001390"
     CDS             complement(21543..22637)
                     /locus_tag="KLK84_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBE2008775.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22670..23668)
                     /locus_tag="KLK84_001391"
     CDS             complement(22670..23668)
                     /locus_tag="KLK84_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE2008776.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23693..25204)
                     /locus_tag="KLK84_001392"
     CDS             complement(23693..25204)
                     /locus_tag="KLK84_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBE2008777.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVTNGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25227..26210)
                     /locus_tag="KLK84_001393"
     CDS             complement(25227..26210)
                     /locus_tag="KLK84_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBE2008778.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(26307..29408)
                     /locus_tag="KLK84_001394"
     CDS             complement(26307..29408)
                     /locus_tag="KLK84_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HBE2008779.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFYPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            complement(29415..29588)
                     /locus_tag="KLK84_001395"
     CDS             complement(29415..29588)
                     /locus_tag="KLK84_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2008780.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            29706..30899
                     /locus_tag="KLK84_001396"
     CDS             29706..30899
                     /locus_tag="KLK84_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBE2008781.1"
                     /translation="MRACINNQQILHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31097..32350)
                     /gene="glyA"
                     /locus_tag="KLK84_001397"
     CDS             complement(31097..32350)
                     /gene="glyA"
                     /locus_tag="KLK84_001397"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBE2008782.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32677..33867
                     /gene="hmpA"
                     /locus_tag="KLK84_001398"
     CDS             32677..33867
                     /gene="hmpA"
                     /locus_tag="KLK84_001398"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HBE2008783.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33912..34250)
                     /gene="glnB"
                     /locus_tag="KLK84_001399"
     CDS             complement(33912..34250)
                     /gene="glnB"
                     /locus_tag="KLK84_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HBE2008784.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34311..35645)
                     /gene="glrR"
                     /locus_tag="KLK84_001400"
     CDS             complement(34311..35645)
                     /gene="glrR"
                     /locus_tag="KLK84_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HBE2008785.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35635..36348)
                     /gene="qseG"
                     /locus_tag="KLK84_001401"
     CDS             complement(35635..36348)
                     /gene="qseG"
                     /locus_tag="KLK84_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HBE2008786.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36513..37940)
                     /gene="qseE"
                     /locus_tag="KLK84_001402"
     CDS             complement(36513..37940)
                     /gene="qseE"
                     /locus_tag="KLK84_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HBE2008787.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFDPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38516..42403)
                     /gene="purL"
                     /locus_tag="KLK84_001403"
                     /gene_synonym="purI"
     CDS             complement(38516..42403)
                     /gene="purL"
                     /locus_tag="KLK84_001403"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HBE2008788.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     ASLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLSLGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42662..44218
                     /gene="mltF"
                     /locus_tag="KLK84_001404"
     CDS             42662..44218
                     /gene="mltF"
                     /locus_tag="KLK84_001404"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HBE2008789.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44215..44751)
                     /gene="tadA"
                     /locus_tag="KLK84_001405"
     CDS             complement(44215..44751)
                     /gene="tadA"
                     /locus_tag="KLK84_001405"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBE2008790.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAVGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44776..45411)
                     /gene="yfhb"
                     /locus_tag="KLK84_001406"
     CDS             complement(44776..45411)
                     /gene="yfhb"
                     /locus_tag="KLK84_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HBE2008791.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45620..46468
                     /locus_tag="KLK84_001407"
     CDS             45620..46468
                     /locus_tag="KLK84_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBE2008792.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46807..47334)
                     /locus_tag="KLK84_001408"
                     /pseudo
     CDS             complement(46807..47334)
                     /locus_tag="KLK84_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11286 a        12961 c        11900 g        11187 t
ORIGIN      
        1 cttgcaggct ggcagcgcga tgacctgcta ttagaagaag gtgcagtaga gctgccggaa
       61 ggagagttta acgccgcgtt taatcctgaa gcagtggtga aagtaaccga tgtgttattg
      121 gcaagccatg aaaatacggc gcaaattatt gatgcccgcc cggctgcacg ttttaacgca
      181 gaagttgatg aacctcgccc aggtttacgt cgcggacata ttcccggtgc attgaacgtt
      241 ccgtggacgg aactggtgcg cgaaggcgaa ctaaaaacga ccgatgaact ggatgcgata
      301 ttttttggtc gcggcgtcag ctatgacaaa ccgattatcg tcagctgcgg ctctggtgta
      361 acggcagccg tggttttgtt agcactcgcg acgctggatg tgccaaacgt gaaactgtac
      421 gacggcgcat ggagtgaatg gggcgcgcgg gcagatttac cggttgagcc agtgaaataa
      481 gtattttaca ggcaataaaa aaccgccgaa tttggcgttt ttttattgct ggtctggttc
      541 gcggcctttc cagcaggttg acttgtgtta cacatgagca acgcaggtgc ttcacagcaa
      601 aacaatactc accagtaact ctctttttgt caagcaaaag agagtaatta ttgtttattt
      661 agcgtattat cgacaccggc cctttccgcc gtgttcggta ataaaataac ctggcttatt
      721 agtccgaatt cagacaaata taaacaaatc ctgctcaaaa ttaaaaattc taaccggtaa
      781 aagatattac ttaaacatgt aaatttactt tcctttaaaa aacaaaaaac cgccaaaatc
      841 aggcggttat ttgttgctgg tccggttcgc ggcctttcca gcaggttgta ttaccgtagt
      901 aatgcaagcg cgtctcagcg gagacaatac tcgccagtaa ctctcttttt gtcaagcaaa
      961 agagagttat tattgttctg ttagtgtatt atccactgcg gccctttccg ccgtctcgca
     1021 agcgggcgct ggcttaagga aaggatgttc cgcgaccgtt agagcaagcg cgtctcagcg
     1081 cttgctatcg cggcaatatc gccagtggtg ctgtcgtgat gcggtcttcg catggaccgc
     1141 acaatgaaga tacggtgctt ctgtatcgta cttattgttt ctggtgcgct gttttcccaa
     1201 gtaaataata accggagtct ctccggcgac aatttactgg tggttaacaa ccttcagagc
     1261 agcaagtaag cccgaatgcc gccctttagg gcggcatatt ttagattatc cggttttgtt
     1321 taaagtcacg caaaaaacca ccccagcgac gttcatagaa tggcgcaata tgttcggtaa
     1381 taaagtggct aattcctttt tccccttttt tcacctgaca aatatcgatt ggttcatcgc
     1441 caggtaatgt atcggtcgct acacttcccg tcgcctgaat aatttcttcg atatcaccat
     1501 cggcttcaat gccaataagt aaattaggct gtgcctcttc gttctcttta attgaacaaa
     1561 taaaagcacg cttcaccggc ttaatggttt taaataaggt ggtgagtgaa tcaatcattt
     1621 gtgctggcgg ctctgcgact tccgataata tcagcgattc accgccttcc aggatttcct
     1681 ggctgctcag cggatttccc tcttcaccaa tcaacaaact gatttcacgc ggcataaatt
     1741 ctttaccggt cggcagtttg gcattaagga agagcgtttc gccaagggtc atctcaaaca
     1801 atgtgcgaac gggcattacg acaaatgcct gttcgtcttc aaccgcctgt tgaagcgctt
     1861 ctaacgaggt gaaaaaagga atgacgctgg tgccgtcttc tttttcccag tgctgtaaat
     1921 caagcgcact atcttcaacc acagcctcgc cctgcgccgc cgtaccaggc acccagacgg
     1981 tggattccag tagagtacgg aaaaaggccg ggcggtgcgc cggttcggtt gctgcttttt
     2041 ccagcaggtc ttcaagttcg ttttttgttt cggacataag aaccacaatt cattcaacgt
     2101 tcggggcgca aatgcctgat gcgctacgct tatcaggcct acaagggatt cgcaatttgt
     2161 tgaatttgca gaatttgtag gccggataag gcgtttacgc cgcatccggc atattagttt
     2221 acgccgttaa cagattagct atcgtgcgca caccaagtcc cgtagcgccc gcagaccact
     2281 gttcaaccgg cgctttacgg taagtcgccg agcagtcgat atgcagccag ccttgctgat
     2341 agttctcaac aaagtgcgac aggaagcccg ccgccgtgct cgcgcctgcc ggatacgccg
     2401 cgcttccggt attgttcagt tcggcaaagt tagacggcag ctggctgcgg tggaattccg
     2461 ccagcggcag acgccagaat ggttcgttct cttgtgaggc actcgccagt aaacgaccgg
     2521 caagcgcatc gtcaaaactg aacagcgcgt gataatcatt acccagcgca gttttcgccg
     2581 ccccggtgag ggtcgccgca tcaatgatca tttccggttt ctgcgcactg gcatcaatca
     2641 gaccatcggc aagcaccaga cgcccttcgg catcagtgtt catcacttca acttttttac
     2701 cgttgcgata ggtgatgata tcgcccagct tgaacgcatt gccgctaatc aggttatccg
     2761 cacagcagag gaacagcttc acgcgcttgt tcagtccgcg cataatggca aatgccagcg
     2821 ccccggtaac cgttgccgcg ccgcccatgt ccgacttcat cgagtccata aacgcagtct
     2881 gtttgatgct gtagccgccg gagtcaaaag tgataccttt acctaccagg cacgcgtaca
     2941 ccggtgcttc tttatcgcca gttgggttgt aatccagcgc cagcaatacc ggagaacgtt
     3001 ctgaaccgcg cccgacagtg tgcagcccca tataaccttg ctcacgcaga tcttcgcctt
     3061 tggtgatccg ataagtcaca cgatcgcccg cgacgttgct gatcagatca acagcacgct
     3121 gtgccagttg cgatggtccc aattcttctg ccggtgcgtt gatggtgtca cgcacccagt
     3181 cgatgatcat caggcggtta tccagttcct ggcgctgggc atcgtccaga tccggccact
     3241 ccactttacg cgtgcctttc gggcctttat aaccttgcca gaatgcccag cagcgatccg
     3301 catcccagcc ttcaccgctt aactgaacat gcttgatgcc cagaccgtca atcttgcggg
     3361 ccgcacgctg gatcagcccc agatcgtctg ccccgttcaa atgcagggta atgccgtcat
     3421 tattaatgct gtaagttgct ttttctcccc agcgcgcgtc ggcaggttgg gtagagaggg
     3481 taatcttcat cgcttctgtc attttagtta tccttattcg tttaacagca aaacgggcta
     3541 cccccaggca gcccgttcat tcactcggct tcgtccagcc agactaacaa aatcgcttcg
     3601 aggattttct cgttggatgc ctgcgggtcg tcgtcgaaat cttccagatc gcaaatccac
     3661 tgatgcatat cggtgaatcg aaccgttttc ggatcaagat cgggatacgc atcgtacagt
     3721 gcttcgccaa tttcgcggct atcggtccac ttaagtccca tactaacctc tgttaatgct
     3781 cacgcgcatg gttgatagtg taacgcggga tttcgaccac taaatcttcg tcggtgacgc
     3841 gcgcctggca gcttaaacgg ctttccggct ccagtcccca ggctttgtcc agcatgtcgt
     3901 cttcctgctc tgagctttcc ggcagtgagt caaaaccttc acgaacgatg cagtggcagg
     3961 tggtgcaagc acaggatttt tcacaggcgt gttcaatctc aataccgtta cgcagcgctg
     4021 cgtcgagaat ggtttcaccg ctattagctt ccagaacagc gccatcaggg cagaggtcct
     4081 gatgaggcaa aataacaatc tttggcatat taaacctcgt ccacggaatg gcctttcagc
     4141 gcacgacgaa ccgactggtc catgcggcga gcggcgaaat cctgggtttg tttgtctacg
     4201 tttttaatcg cttgttcgat ggcgtcaaca tcatcgccct gcgccacttc actcaggtga
     4261 gcggcagcat tgtcaatgac ctgacgctct gcggcgctta acagcgcggc atcagcagcc
     4321 agcgcgccgt gcagactttc cagcacacgc gccgcttcta ctttttgttc tgccagcata
     4381 cgggctttta cgtcctgctc ggcatagctc attgagtctt tgatcatcga agcgatttcg
     4441 ctatcggtca gaccgtaaga cggtttgacc tgaatagacg cctcaacgcc ggtggatttc
     4501 tccatcgccg tcacgctcaa aagaccgtcg gcatcgacct ggaacgtcac gcgaatatgc
     4561 gcaccgccag ccggtagcgc cggaatacca cgcagcgcaa aacgcgccag tgagcggcag
     4621 tcctgcacca gttcgcgctc accctgcatt acatggatag acatcgccgt ctgaccatct
     4681 ttaaaggtgg tgaaatcctg agcgcgggcc accggaatag tggtattacg cggaatcact
     4741 ttctccacca ggccgcccat cgtttcgagg cccagcgaca gtgggatcac atcaagcagc
     4801 agcatttcgc tgtctggctt gttacccacc agaatatccg cctgaatcgc cgcgccaata
     4861 gcgacgactt tatccgggtc gatggaagtc agcggtggac gaccgaaaaa ttcgcctacc
     4921 cgttcacgca ccagcggcac gcgagtagaa ccgcccacca tcaccacttc cagcacttcg
     4981 tcagcttcta cacccgcgtc tttcagcgcg cgacgacaag ccagtaaggt tcgttttacc
     5041 agtggcgcga tcagttcatt gaattgttca cggctgattt cgccctgcca gcccgcaacg
     5101 ttaacggtca cggagtccgc atcgctcagc gcgattttgg ctgcaatggt ggcatccagc
     5161 agttcacgct gaacgcggtt atcgctacga tcaggaatgt ccgcctgctc gcgaatgtaa
     5221 tccgccagca gatggtcgaa atcatcgccg ccgagcgcgg aatcaccgcc ggttgccagc
     5281 acttcaaaca cgccgcgact taagcgcaga atggaaatat caaacgtccc gccaccgagg
     5341 tcataaacag cgatcacgcc ttcctgaccg gaatccagcc cgtaggcgat agccgcagca
     5401 gtcggttcgt taagtaagcg cagaacatgc aaccccgcca gacgcgccgc gtctttggtg
     5461 ccctgacgct gggcatcgtc aaagtacgcc ggaacggtga taactacacc atccagctcg
     5521 cctgccaggg cttcagttgc ccgcgccgcc agtgctttga ggatgtccgc agaaacgcgc
     5581 accgggttca gtagccccgc cgccgtttca atcatcggca ggccgttttc gctggcctgg
     5641 aattgataag gcagatgcgg atagcgttgc tggatatcag ccagcgagcg ccccatcagg
     5701 cgtttaacag aactaatggt gttggcggtg tcaagcgctg cgttagtacg cgcgtcataa
     5761 cccaccgaat gcccttgctg ttgatagtga acaacagatg gcagcaggtg acggccttca
     5821 tggtcggcta acgtttcggc ctgaccgctg cgcactgtcg ccaccagcga gttggttgtg
     5881 cccaggtcaa taccagccgc cagacgacgc tgatgcggcg cagcactcaa accaggttca
     5941 ctaatttgta ataaggccat gtttagcttc cagaaattaa aaatcgagca gtttttcttc
     6001 gagttgttcg gcactgctcc gcagtttatc gagaaaacgc agcttacgca ccgtatccgc
     6061 cgccgcgtcc cacgcctcgt tgtctaactg ttcaaccatc aactgatggc gggtatcaaa
     6121 catctttttc acacgtttga taaagctttc cagccgcgct tcatctttcg cctgttcgat
     6181 ctcgtccagc tcttcgcgca gctccaactg ttccatcagg aacgcggtgt cgcgcacagt
     6241 atgctgctcg ctggcgagat caaaaccgtg taaagaaagc aaatattccg cgcgcattaa
     6301 cggatgacgc agcgtttgcc aggcctggtt aatggttgca gattgctgta cggcggcgag
     6361 ttgttccgcc aggcttccgc tggcgaattt atcaggatga tactgacgtt gtagatcctg
     6421 aaaacgcagg ctcagtgcct gggtatcgag ttgataacgg gcaggcaagc caaagagggt
     6481 gaagtaatcc ataacattct cagggctgcg atccgccgcg ataacggcga atcaggtaaa
     6541 acaaacccca cgcgcaggcg accacggtgg ggttatcggt atgcgcatca aacgtggaag
     6601 ctttcgccgc aaccacactc atctttaacg ttcgggttgg tgaatttaaa cccttcattc
     6661 aggccttctt ttacgaagtc cagctgcgta ccgtccagaa attgcaggct tttgccatcg
     6721 accacgactt tcacgccttt gtcttcaaac acgatgtctt ccggcgtcgg ttcgtcaaca
     6781 aattccagta cataagccat acctgaacat ccggaggttc tcacgcccag acgcaggcca
     6841 aaccctttgc cgcggttagc cagaaaggta tttactcgcg ctgctgcact gtcgctcagt
     6901 gtaatcgaca taaccaaacc tcaactctta ttttgcttca cgtttgcttt tatagtccgc
     6961 aatggcggct ttgatcgcgt cttctgccag aatagaacag tgaattttca ccggcggcag
     7021 ttcaagttct tcagcaatat cggtgttttt gatcgcctgc gcttcgtcga gagacttccc
     7081 tttcacccat tcggtgacca gggagctgga agcgatagcg gaaccgcagc cgtaagtttt
     7141 aaaacgcgcg tcttcaatga taccttcatc gttgacttta atctgcaact tcattacgtc
     7201 gccacaggcc ggtgcaccca ccatgccgct gccgacgttc tcgtcgttgt tatcaaagga
     7261 acccacgtta cgcggattct cgtaatggtc gataactttt tcgctgtaag ccattataaa
     7321 ttctcctgat tccgataccg attaatgatg agcccattcg atgctgttca gatccacgcc
     7381 ctgcttgtac atttcccaca gcggagaaag gtcacgcaga cgaccgatgg atttacgaac
     7441 taactcgatg gtgtagtcga tctcttcttc agtagtaaaa cgacctaaag agaaacggat
     7501 agagctatgt gccagctcgt cgttcagccc cagcgcgcgc agcacgtagg acggttcgag
     7561 gcttgctgac gtacaggcgg aacctgaaga aactgcgagg tctttcagcg ccataatcag
     7621 cgactcacct tcaacgtagt tgaagctgac gttgagaatg ttcggcgcac cgtgttccag
     7681 gtcaccgttc aggtaaactt cttcgatatc tttgatgccg ttccacagac ggttacgcag
     7741 gccgcgcaga cgttccatct cggtcgccat ctcttctttt gcgatgcgat aggcttcgcc
     7801 cattccgacg atctggtgaa caggcagagt gccggaacgc ataccgcgct cgtgaccgcc
     7861 gccgtgcatt tgcgcttcga tgcgtacacg cggtttacga cgcacataca gcgcaccgat
     7921 acctttcggg ccatagattt tgtgaccgga gaaagacatc aggtcaactt tcaactggct
     7981 caggtcgatt ggcagtttgc ccacgctctg ggttgcatca acgtgataga taatgccacg
     8041 agcacggcac atttcgccga tagccgcgat atcctgcacc acgccgattt cgttatttac
     8101 gtgcatgatg gaaacaagga tggtgtcgtc acgcatcgcc gcttcgagtt ctttcaggtc
     8161 gataataccg ttacgctgcg gtgccaggta ggtgacttcg aaaccttcgc gctccagctg
     8221 acggcaagta tccagtaccg ctttgtgttc ggttttgctg gtgatgatgt gcttgccttt
     8281 tttctgataa aagttggctg cacctttgat cgccaggttg tcagattcgg ttgcaccaga
     8341 ggtaaagacg atttcacgcg gatcagcgcc gaccagatcg gcaatctgat tacgggcgat
     8401 atctaccgct tcttcagcct gccagccgaa acggtgagaa cgagaggccg ggttaccaaa
     8461 ggttccgtcc atcgtcatga actgcatcat tttctcggca acacgcgggt ccaccggcgt
     8521 ggttgcggag tagtcgagat aaatcggtaa tttcattgct ctataaactc cgtacatcac
     8581 tcaatgcaag gaatcaggct accggctgga tgtacgaccg tgtttacgga gtatttagca
     8641 ctccggcctg attctgaatt ctttttatta agcgcgtaac ttaacgtcga tcgcgtcttg
     8701 tgtgcgggtg cgtggcgcgt cgtgagtatg ctgacgacca gacacatcca gcacttcctg
     8761 gttattaacc agttcgccta aagtaatgtt gttgagaaaa ccggtgagac ggtcgctcaa
     8821 atcacgccac agcgcgtggg tcaggcattt atcgccgccc tggcagccgc ctttaccctg
     8881 acaacgggtg gcatctacag attcgtcaac ggcgctaatc acttcgccaa cggcgatgct
     8941 gctggcatct ttgcctaaca gataaccacc gcctggtcca cgtacgctgg aaaccagacc
     9001 atttttacgc agacgggaaa acagttgttc cagataagaa agggaaattc cctgacgttc
     9061 ggaaatatca gccaacggta ccgggcccgc ttcagagttg agcgcaacgt caagcattgc
     9121 ggtcacggca tagcgccctt tagatgtcag tctcatgtct tacttcacct caaactcgcc
     9181 cctgcccggg gttttttatt gtaaaagtgg gggtattgca tagcagggtc aagtctgaca
     9241 ttcccgagta aattggtcaa ctatttactt gactgattta gtcgggtatt taaccttcag
     9301 tgccattttt ttatcgtggc gtttgtacct gttgtcggat gcggtgtgaa caccttatcc
     9361 gacctacgat tctgtacatt gtaggcctga taagacgcgt taagcgtcgc atcaggcaac
     9421 cactgttatt cggccttgtt acctttattc tgctgctcaa tagaagccag aatcccgcgc
     9481 aggatattca attcctggct ttccgggcgc gcacgggtaa acagacggcg caatttattc
     9541 atcacctgcc ccggatggtt ttcacggata aaaccggttg ccagcagcgt ttgttccaga
     9601 tgaccataaa aacgctccag atcatcgacc agcggatacg gcgtctcttc atgctcgacc
     9661 tgctcgccat tttcctgagt tgccagccag gccatacgca cttcataggc aataacctga
     9721 accgccattg ccaggttcag cgaactgtat tccgggtttg ccgcaatcgc gacatgataa
     9781 tggcatttct gcaactcttc attggtcaag ccgacgcgct cacgaccaaa caccagcgcc
     9841 accggggtat ttgccgcttc agcgacgctt ttcagaccgc attcgagcgg gtcgagcatc
     9901 ggccacggca gcgtgcggga gcgtgcgctg gtgcccacca ccaggctaca accggctaac
     9961 gcttcgtcga gcgtatcgac gatgtgagca ttaccaatca catcgctggc ccctgcagcc
    10021 agggcaatcg cctgggagtc gggtttcacc agtggattaa ccagccacag attggttaat
    10081 cccattgttt tcatggcacg ggcaacagaa cccatattgc cggtgtgtga cgtctccacc
    10141 agcacaattc gaatattttg cagcattgtc tttcttcatc taaagattat tcacgcatct
    10201 tatcataaaa cgaagacaga tgccgatctc gctgctatac tctgcgccgt tttcccgttc
    10261 tttaacatcc agtgagagag accgatgcat ccgatgctga acatcgccgt gcgcgcagcg
    10321 cgcaaggcgg gtaatttagt tgccaaaaac tatgaaaccc cggacgctgt agaagcgagc
    10381 cagaaaggca gtaacgattt cgtgaccaac gtagataaag ctgccgaagc ggtgattatc
    10441 gacacgattc gtaaatctta cccacagcac accatcatca ccgaagaaag cggtgaactt
    10501 gaaggtactg atcaggatgt tcaatgggtt atcgatccac tggatggtac taccaacttt
    10561 atcaaacgtc tgccgcactt cgcggtatct atcgccgttc gtatcaaagg ccgcaccgaa
    10621 gttgctgtgg tatacgatcc tatgcgtaac gaactgttca ccgccactcg cggtcaaggc
    10681 gcacagctga acggctaccg tctgcgcggt agcaccgctc gcgatctcga cggtaccatt
    10741 ctggcgaccg gcttcccgtt caaagcaaaa cagtacgcca ctacctacat caacatcgtc
    10801 ggcaagctgt tcaacgaatg tgcagacttc cgtcgtaccg gttctgcggc gctggatctg
    10861 gcttacgtcg ctgcgggtcg tgttgacggt ttctttgaaa tcggtctgcg tccgtgggat
    10921 ttcgcggcag gcgagctgct ggttcgtgaa gcgggcggca tcgtcagcga cttcaccggt
    10981 ggtcataact atatgctgac cggtaacatc gttgctggta acccgcgtgt tgtgaaagcc
    11041 atactggcga acatgcgtga cgagttaagc gacgctctga agcattaatg actcaggcgg
    11101 gtgatatcac tcacccgccc tcgcctttca ggcgctattc cgaaatactt cctcaccgct
    11161 ttactttctt tcgtcactct cccaccatct ttccccgatt aatggatgat aaagaagtcg
    11221 caaggaaagg ttatggcact gccagtgaac aaacgcgttc ccaaaattct gtttattctc
    11281 tttgttgttg ccttctgcgt ttatttagtg ccgcgcgttg ccatcaactt cttctattat
    11341 cctgacgata aaatttacgg tcccgacccc tggtcggcgg aatccgtcga atttacggct
    11401 aaggacggta ctcgtctgca aggctggttt attccttctt cgacgggccc tgctgacaac
    11461 gccatcgcaa ccatcattca tgctcacggc aatgccggaa atatgtccgc ccactggccg
    11521 ctggtcagtt ggttacctga gcgtaatttc aacgttttta tgtttgatta tcgcgggttt
    11581 ggtaaatcaa aaggcactcc gtcccaggcc ggattgctgg acgatacgca aagcgccatc
    11641 aatgtggtgc gccatcgcag tgatgtaaac ccacaacagc tggtgctgtt cgggcagagt
    11701 attggcgggg cgaatattct tgctgttatt ggtcagggtg atcgtgaagg catacgtgcg
    11761 gtgatcctcg actccacatt tgcctcttat gcaaccatcg ccaaccagat gatccccggc
    11821 agtggctact tacttgatga gagttacagc ggcgaaaatt acatcgccag cgtcagcccg
    11881 atcccgcttt tactcattca cggtaaagcc gatcacgtta tcccatggca gcacagcgaa
    11941 aagttgtata gcctggcaaa agagccaaaa cggttgatcc taatcccgga tggcgaacac
    12001 attgatgctt tttccgatcg tcacggcaat gtttatcgcg aacagatggt ggactttatc
    12061 cttagcgcgt tgaatccgca gaactaaccc atgatcgcta gcacgataat cattcacaaa
    12121 accaccttaa gacatgctaa tccactggtc agaacagttt aaaatgagaa aaattctgtg
    12181 acgcttgcca acatttctga tgattagcat tcccttcgcc atttccctga gcaaacttta
    12241 gctattctta tcaattatgc ttatgggaga tctacagatg atgcctacgc ttgctccacc
    12301 atctgtcctt tcggctcccc agcgccgctg ccagatcttg ctgacgctct ttcagccggg
    12361 gttgaccgcc accatggcaa ccttcagcga gcttaatggt gtggatgatg atattgccag
    12421 tcttgatatc agcgaaacag gacgggagat cctgcgctat catcaactca cactgacgac
    12481 tggttatgac ggtagctacc gggttgaagg tacagtgctt aaccaacgtt tgtgtttatt
    12541 tcactggcta cgacgtggtt tccgtctgtg tccgtcattt attaccagcc aattcacccc
    12601 cgccctgaag agtgaactga agcggcgcgg aattgcgcgt aacttttacg acgataccaa
    12661 tctacaagcg ttagtgaatc tctgctcccg acggctgcaa aaacgctttg aatcgcgcga
    12721 tattcatttc ctgtgtctgt atctgcaata ttgtttgctg cagcaccacg ctggaattac
    12781 gcctcagttt aatccgctcc aacgtcgctg ggcggaatcc tgccttgaat ttcaggtagc
    12841 gcaggaaatt ggacgccact ggcagcgtcg ggcgctccag catgtaccgc ctgatgagcc
    12901 actgtttatg gcattacttt tttccatgtt gcgggttccc gatccactgc gggatgcgca
    12961 tcagcgggac agacaattgc gtcagtctat caaacgtctg gtaaaccatt ttcgtgagct
    13021 gggaaatgtt cgtttttatg atgaacaggg gttatgtgat cagctttata tccacctcgc
    13081 ccaggcgtta aatcgcagtt tgtttgccat cggtattgat aataccctgc cggaagagtt
    13141 cgccagactg tatccacgcc tggtacgcac cacccgcgcg gcgctggccg gatttgaaag
    13201 tgaatacggc gtccatcttt ctgatgagga aagtggtctg gtcgcggtga ttttcggtgc
    13261 ctggctaatg caggaaaacg atctgcatga aaaacagatt attctactga ccgggaatga
    13321 tagcgagcga gaagcgcaga ttgagcagca gttacgcgaa ctaacgttac tgccgctcaa
    13381 cattaagcat atgtcggtaa aggcattttt gcagacaggc gctccgcgtg gcgcggcact
    13441 gattattgcg ccttatacca tgccgttacc gctcttttca ccaccgctga tctatacgga
    13501 cctgacgctg acaacacatc aacaggagca gatccgcaaa atgctcgaat cagcatgagg
    13561 gaacaacttt cgggcgcaaa aacattgccg gaagcgccac cagcgccatt acccagaaca
    13621 cgccgtggcc cagatattga tacaggaaac cggcgaaaac ggtcatgata gcgatactgc
    13681 cgcccatcgc gacggcagag taaaccgcct gtaaacggat gacttcgcta acctggcgag
    13741 cagcaatata acgcatggcg gccaggtggc agaccgtgaa ggtgccgcaa tgcagaattt
    13801 gcaccactat caaccacggc aacgccgtag ttgctcccat aatgccccag cgcactacgc
    13861 cgcaaatcgc cgagatcaac agcatatcgc gagcactaca acggcggaaa agtttattac
    13921 tcagcgcaaa gataatgact tccgccacca cgcccagcga ccacaaatac cccaccgccg
    13981 aggccgagta gccagctgcc tgccagtaaa tggcgctaaa accgtaatag gccgcatgtg
    14041 ccccctgcaa taaacaaacg caggccagaa agcgccagtt ctggcgaacc agcgccaacc
    14101 acgcagacca accggtgctc tcctgctggc ggcttgcccc ttgtggctga atcgtcggac
    14161 ggatgagaaa gccgagcagc atggatgcca cgcccaacgt caacagcacg agaatcaccc
    14221 gataatcaaa catagtgacc agtttgcccg tcagcgccga gccaatgaca aacgccaccg
    14281 agccccacag tcgcactttg ccgtaatcaa gcgggaactg cttttgccac gtattcgcca
    14341 gtgcatcggt caacggtacc agcggtgaga aaaagaggtt aaagccaatc atcaccagca
    14401 tcagccacgc tacgtgcgcc cccgcccaga aggcgacagc aaagagaagt ggcagcagtg
    14461 ccagcacgcg caaggcggaa atcaggcggg aaggatcgct gacgcggggc gcgatgagca
    14521 aactcccgag aaaacgggca accagacctg cccccaataa caggccgatg gtttctggcg
    14581 ttaaaccaat ccctttaagc cagacgctcc agaaaggtaa aaaaatgccg taactaaaaa
    14641 agtatgtgaa atagccgagc gccaaccagc gcgtggattg caaaaccatg agtccctccc
    14701 gtcaggaggc gttagtctgg ggttaatcga atgttggtgc aagttgaaaa ataagaatat
    14761 tcgagcctgc ttcccgatgt ctgtaacgcc agttcacaag cactaaaaaa ctgcgatccg
    14821 cgacgcatgt tgcaaaaata atgcgtaatt atgccgttac gcttgccaaa cgttcctgca
    14881 caatggcgat gaaatcgcgt aatgccagct tcatctcccc tttcttccac gccatcagta
    14941 aagcaatgga aggcacattt ccggcaatgg gacgaaaaac aacctgcccg gtattaaaat
    15001 tattcatata accgggtatc aaagtgacgc ccagccccat gcccaccaga ttcatggtca
    15061 ccagaatatt cgttgccacc tggacgatat ttggctggct gttttcttgc gcaaaccacg
    15121 ctttaacgat cggcgcaagc gaaccggaat acgccggatc ggtactgacg aaattcacgc
    15181 catccagttg cgcggcggtg atctcttttt catgtgctaa agggtgatca accggtaaca
    15241 ccaccactaa tggttcgtca aaaagctcca gataatcaat ctccgggcta taaacaggat
    15301 ggcgcattaa gccgacgtcg agttcaccac ggcgaatttt ttcctcctgt tgcgtggtga
    15361 ttaaactcac cagctcaatc aaggtgtctg gctgtctgag acgaaacatc ggtaatactt
    15421 ttggcaataa attcacttcc gccgatggca caaagccaat ggttaattgt ctgtcttcct
    15481 gaacaatttt ccgcgcccgt aatttggcat tttccgcttg ttcgaggatt gccagcgcgt
    15541 cctggagaaa acattctccc gccgccgtca acgcgacttt gcgcttatcc ctcaccagta
    15601 acggaacacc gacacagttt tcaagatcgc ggatctggct gcttaacgaa ggctgtgagg
    15661 tatgcagttt ttccgccgca cgggtaaagt tcagtgcctg cgccactgcg acgaaatagc
    15721 gtaaatgccg tagttccatc accttcccct tgttatcgaa aaaacgtctc agctggtaga
    15781 aattaactat ttcacatatt agcaaccaac cagcaacatc cttatggcac aaaaatagaa
    15841 ggtcaataca tcttatcttt caggattaaa aaatatgacc acaccctcag atttgaacat
    15901 ttaccaactg attgacaccc aaaacggtcg ggtcactccg cgtatttata ccgacccgga
    15961 catttaccaa ctggagcttg agcgtatttt cggtcgttgc tggctatttc tcgcccacga
    16021 aagccagatc ccaaaacccg gtgatttctt taacacctac atgggagaag atgcagttgt
    16081 cgtggtgcgc cagaaagacg gcagcatcaa ggcgtttctc aaccaatgcc gccaccgggc
    16141 catgcgtgtg agttatgcag attgtggcaa cactcgcgcc tttacctgcc cgtatcacgg
    16201 ctggtcttat ggcattaacg gcgagttgat cgatgtaccg ctggaacctc gcgcctaccc
    16261 acaagggttg tgtaaatccc actgggggct aaacgaagtt ccttgtgtgg agagttataa
    16321 aggactgatt tttggcaact gggataccag cgcaccgggc ctgcatgatt acctgggtga
    16381 cattgcctgg tatctggatg gcatgctgga tcgtcgcgaa ggcggcaccg aaattgtcgg
    16441 cggcgtacaa aagtgggtga tcaactgtaa ctggaaattc ccggcagagc agttcgccag
    16501 tgaccagtat catgctctgt tcagccatgc ttctgccgtt caggtattag gggcgaaaga
    16561 tgatggcagc gataagcgcc tcggtgatgg acaaaccgcc cgcccggtgt gggaaaccgc
    16621 caaagatgcg ctgcaatttg gtcaggacgg tcacggtagc ggtttcttct ttactgaaaa
    16681 accggatgct aatgtctggg tcgatggcgc agtttcaagc tattaccgcg aaacctatgc
    16741 cgaagcagaa caacgtttag gtgaagttcg cgccctgcgc ctggcgggtc ataacaatat
    16801 attccccacg ctttcatggc taaacggcac tgccacactc cgcgtctggc atccgcgcgg
    16861 ccctgatcaa gttgaagtgt gggcgttctg tattactgac aaagccgcct ccgatgaagt
    16921 taaagccgct tttgaaaaca gcgccactcg tgcttttggt cctgctggtt ttctcgagca
    16981 ggatgactcg gagaactggt gtgaaatcca gaaattgctt aaaggccacc gcgcccgcaa
    17041 cagcaaactg tgtctggaaa tggggcttgg tcaggaaaag cgccgcgacg acggcattcc
    17101 tggcattact aactatatct tttcagaaac tgccgctcgt ggaatgtacc aacgctgggc
    17161 cgatcttctg agtagcgaaa gctggcagga agtgctcgat aaaaccgccg cttaccagca
    17221 ggaggtgatg aaatgagtgc gcaagtttca ctggagttac atcaccgcat tagccagttt
    17281 ctctttcacg aagccagtct gctggatgac tggaaatttc gtgactggtt ggcacagctc
    17341 gacgaagaga ttcgttacac catgcgcacc acggttaacg cgcaaacacg cgaccgtcgc
    17401 aaaggcgtcc agccgccgac gacctggatt ttcaatgaca ccaaagacca gctggagcgg
    17461 cgaatcgccc gtctggaaac gggcattgcc tgggcagaag agccgccgtc acgcactcgt
    17521 cacttaatca gcaactgcca ggtaagcgaa accgacatcc caaacgtatt tgccgtgagg
    17581 gtgaattatc tactttatcg ggcacaaaaa gagcgcgatg aaacattcta tgttggaacg
    17641 cgtttcgaca aagttcgccg tctggaagat gacaactggc gcttgctgga acgggatatc
    17701 gtcctggatc aagcggtgat cacttcccat aacctgagtg tactgttctg atgaatcgaa
    17761 tttatgcgtg tcccgttgcg gatgtgccgg agggtgaggc tctccggata gatacctcgc
    17821 ccgtcatcgc cttgttcaac gtgggcggcg agttttatgc cattaacgat cgttgcagcc
    17881 atggtaatgc gtcgatgtca gaagggtatc tggaagatga cgccacggtg gagtgcccgc
    17941 tacacgccgc cagtttttgc ctgaaaacgg ggaaagcgtt atgcctgccc gccaccgatc
    18001 cgctcaccac ttatccagta cacgttgaag gtggtgacat tttcatcgac ttaccggagg
    18061 cgcagccatg agcgatctgc ataacgagtc catttttatt accggcggcg gatcgggatt
    18121 agggctggcg ctggtcgagc gatttatcga agaaggcgcg caagttgcca cgctggaact
    18181 gtcggcggca aaagtcgcca gtctgcgtca gcgatttggc gaacatattc tggcggtgga
    18241 aggtaacgtg acctgttatg ccgattatca acgcgcggtc gatcagatcc tgactcgttc
    18301 cggcaagctg gattgtttta tcggcaatgc aggcatctgg gatcacaatg cctcactggt
    18361 taatactccc gcagagacgc tcgaaaccgg cttccacgag ctgtttaacg tcaacgtact
    18421 cggttacctg ctgggcgcaa aagcctgcgc tccggcgtta atcgccagtg aaggcagcat
    18481 gattttcaca ctgtcaaatg ccgcctggta tcctggcggc ggtggcccgc tgtacaccac
    18541 cagtaaacat gccgcaaccg gacttattcg ccaactggct tatgaactgg caccgaaagt
    18601 gcgggtgaat ggcgtcggcc cgtgtggtat ggccagcgac ctgcgcggcc cacaggcgct
    18661 cgggcaaagt gaaacctcga taatgcagtc tctgacgccg gagaaaattg ccgccatttt
    18721 accgctgcaa tttttcccgc aaccggcgga ttttacgggg ccgtatgtga tgttggcatc
    18781 gcggcgcaat aatcgcgcat taagcggtgt gatgatcaac gctgatgcgg gtttagcgat
    18841 tcgcggcatt cgccacgtag cggctgggct ggatctttaa ggaagcacga tgaaagaaaa
    18901 aacgatcatt attgtcggtg gcgggcaagc ggcggcaatg gctgcggcct cgctacgcca
    18961 gcaagggttc accggtgagc tgcatctgtt ttccgatgag cgacatcttc cttatgaacg
    19021 acctccactc tcgaaatcca tgttgctgga agattccccg cagttacagc aggtgttacc
    19081 cgctaactgg tggcaggaaa acaatgttca tctgcattcc ggtgtaacca tcaaaacgct
    19141 gggtcgcgac acacgagagt tagtgttaac caacggcgaa agctggcact gggatcagct
    19201 ttttatagca accggcgcgg cagctcgacc gctgccgttg cttgatgcac tgggagaacg
    19261 ctgctttacc ctacaccatg ccggtgatgc cgccagactg cgagaagttc tgcagcccga
    19321 acggtcagtc gtgattgtcg gtgccggaac tattggtctg gaactggctg ccagcgccac
    19381 gcagcgcaga tgtaaggtga cagtgattga actggcggca accgtcatgg gccgtaatgc
    19441 accaccgccc gtgcaacgct atcttttaca gcgccatcag caggctggtg tgcgcattct
    19501 gctcaataat gccattgaac atgtggtcga tggtgaaaaa gtagaactga cgctgcaaag
    19561 tggggaaacg cttcaggctg atgtggtgat ttacggtatt ggtatcagcg ccaacgagca
    19621 actggctcgc gaggccaacc ttgatactgc caatggcatt gtcattgatg aggcttgccg
    19681 cacctgcgat cccgcgatct ttgccggtgg cgatgtggca atcactcgtc ttgataatgg
    19741 tgcactacac cgctgcgaaa gctgggaaaa cgccaataac caggcgcaaa ttgccgctgc
    19801 cgcaatgtta gggctaccgc taccgctact gccgccgccg tggttctgga gcgatcagta
    19861 cagtgataac ttacagttta ttggcgatat gcgtggcgat gactggcttt gtcgtggcaa
    19921 cccggaaact cagaaggcga tttggtttaa tctgcaaaac ggcgtgctta tcggtgcggt
    19981 cacgctgaat cagggccgtg agattcgccc aattcgcaaa tggatccaga gcggcaaaac
    20041 gtttgatgcg aaactgctga tagatgagaa catcgcgctt aaatcactgt aatcaggata
    20101 attagcgaat atctcaatgc ctggggcgtg gcgaggtgca agagtgtgta ttacgtttaa
    20161 atcacattat cttgcaaagg gattggttat gaacacacta cgttattttg attttggagc
    20221 tgcccgcccc gttttgttat taattgcccg tatcgccgtg gtcttaattt tcattatttt
    20281 tggttttccc aaaatgatgg gctttgacgg tacggtccaa tatatggcct cgttgggcgc
    20341 gccaatgccg atgctggcag cgattattgc ggtagttatg gaagtgcccg ccgcgatatt
    20401 aatcgtgctt ggctttttca cccgtccgct ggcggtgctg tttattttct acacgctggg
    20461 tacggcggtg attggtcacc attactggga tatgaccggc gatgcggttg ggccaaatat
    20521 gattaatttc tggaagaatg tcagtatcgc tggcgcgttc ttgctattgg caattaccgg
    20581 accgggggca atttctctcg atcggcgtta ggaaagatgc cggatgcggc gtgaacgcct
    20641 tatccggcat tttataaatt acagccactc tacccgcaac gacatctctg aggttgttga
    20701 ttcccctggt gctagcgcaa tgagatcacc cccttccggg cgatgatgat catccggcgc
    20761 atggctcatc ggttccagac agaagaaatc aaacgcatat cctttatcaa acgcagggtc
    20821 ggaaacaaag atgaaataac acggtgcagg tggcgtcgtt tccatgatga tggcataacc
    20881 ttcctgcggc tgctcgatgc gggcctgacc attccatccg gcaaaaccat tgttcaccca
    20941 ctgacgcggc aacggcgcga gctggttaaa atccagttcc tgcggtagct gctcgcaaaa
    21001 ctcacccgcc agccactgct cccgttccag ccagtaaccg ctcgcctgcg cctgaatccg
    21061 cgtttgtggt gacaacggaa aataaggatg ccagccggta ccaaatggca gcgtctctgc
    21121 cccttgattg gtgacagaga gcgtcaccgt cagcgtatcc gccgttaaat gaaacgcctg
    21181 acttacccga tagtgataga caccgctgcg atgttcatac accagacaca aactatcatc
    21241 gctgtgcgag acacattgcc actcgcccag ccagccatcg ccgtgtagat agtgcgcatc
    21301 ccactcaaca ttcggttgca gttgatactc acgcccctgc cagacaaaac ggttgccgct
    21361 cacccggttt gcaaatggca ccagcggaaa acatgaggca tcggttgcca caccgctttt
    21421 tttaccagga cgtaagagcg tcgtcgtatc gcgccagaag ccttcgatta cgccgccctg
    21481 gtcagaaacg tccagcttta gcgacccgtg ggataaggta tagatggtca tccgctaact
    21541 ccttaatccg ggaagttaat cacaactttc ccgcatttgc cgctcgccat cagcacatag
    21601 gcatcacctg cctgttctag cgagaagcga tgggtaatgg cgttacgcgg ccacagcttc
    21661 cagtcagtca aatcgtgggc gcatttttcc atatggaaca gactggtcac ccaggagccg
    21721 ataatccgcc gttgatggtg catcagatca gcgctgacct cgaattccac ttttccggtt
    21781 tcaccaatgt aaaccacccg cccccagtca gcagtggatt gcagtgccag caagcgacct
    21841 gcggcattac cggaacaatc gagcgcaaca tccgcgccac cgtgggtaag ttcagcaatt
    21901 atctgcggca gaccttcggt ggttgctaaa tagccgtgat ccatcccccc taactgtttt
    21961 gccatcgcca gacgttccgg cagcatatca acgccgatga tccgttttgc accgcgacct
    22021 ttcgccagca tcatcgccat catgccgacc ggccccagac cgaccaccag cacgttatcg
    22081 ctgccggaaa cttcgccgcg caaaatccct tcataggccg tgccaacgcc gcaactgata
    22141 aacgcaccat cttcgtagct cagcgcatcc ggcaggagga tcagatcttt ttcttccgcc
    22201 agcagatatt cggcatgacc gccgtcacgc tgccagccgt aagccgcttt tcctttgcca
    22261 gtacaagaga taggaaagcc gcggcggcag ttcgggcaaa aaccacagcc agaaatgtga
    22321 tacaccagca cgcggtcgcc ctctttaaaa tggcggcagc cttgccccat cgccacaatc
    22381 tgcccgcacg gttcatgacc gttgataaat ccctggtata acggtttatc gggtgccgcc
    22441 gccgtggcac ggtgttgatg atagatatag tggacatcgc ttccgcaaat cccggaggat
    22501 ttcattttga tcagtacctg gttaatcccc ggcgtcggca ccgcaacttc ccgcagatcg
    22561 acggttgaat ttcctagtaa ataagcagcc agcatcgttt tcataaatcc ctcattaaca
    22621 atacgattaa ttttcatccc tgccgcaccc gcgccagggg cgttaaggtt tagcgtttcg
    22681 ctttactgct tcgctgggtc agcaagatat tcgccagcac cgccaccacg atgatgacgc
    22741 cgcgtaccac ctgctggaaa aaggagttaa taccgagcag caccagaccg ttaccgatta
    22801 gcgtaatcac cagcacacca agcaatgtac cgaacaggga accgcgaccg ccggaaagtg
    22861 ccgtaccgcc gaccacgacc gcggcgatga cgtcaaactc cagaccgttt gcggcacctg
    22921 cgttaccgga accgaggcgt gccgccaaca aaatgccggt caccgccgct aataatcccg
    22981 aaagggtaaa gataagaatg cgcactcgac gaacgttaat accgcacaac tgcgccgccg
    23041 tggcattacc gccaacggca aaaaccgagc gcccgaaggc ggtttttcgg ctaatgaaca
    23101 caaacagcgc aaacaacacc atcatgatca gcgcggatac tggcacaccg agaaattgtc
    23161 cgcccagcca gtccagcacc tcgttttcgt caatcggcac tggcagtgcg ttggtcataa
    23221 acagccccat tccgcgcaga gcgctccaca gccccagtgt ggcaacgaaa cttggtacgt
    23281 taaacacgcc gcgcagcacc ccggcgagcg ttcccatcag cgcgcctaac agcaacacca
    23341 gcagacaagc aatcgccaac ggaacttcaa attgcagcaa aaatgccagg cacaccgaga
    23401 caaaagccac catcggccca acgctgacat caatttcacc ggagataata atcagcgtca
    23461 tcgcccaggc ggcaatccca atggtggcgg catcgcgcag cacgttcatc tggttattca
    23521 atgagataaa gccaggcgcg ttcagggaga agaccagata aagaatggca atcaccacca
    23581 gcaaaccgat ctcattaata tggcgactga caaattgttt gagcgagacg ctcttgccct
    23641 gcggcaatgg taatgacgaa gcatacatct tcaggttcct cggtaaaatt catcagtgta
    23701 cagacagaat ggcggacatc agctcatcca cattgaccgg cgagtgaaac tcctgcgaga
    23761 acgtgccgtg ctgtaataac aggatgcggt cacacaccag cggcaattcc tccacttcac
    23821 tggagataaa caccacgctt tttccttcgg cagccagctc acggacaata cggtaaatct
    23881 gctgtttggc ttcgatatcg acgccgcgcg ttggctcgtc gagcaacaaa atctggctgg
    23941 cggcatagac ccaacgaccg atcaccactt tttgctgatt gccaccagaa agcgtgccga
    24001 tgggtgtttc gctactggca gccttgaccg tcatccgctg catcacctct tcggtcaggc
    24061 ggcggatggt ggaccattgc agcacaccgt tggtgctgat tttttgccga ttggtcagca
    24121 ctgtattttc gtcaacgcct agccagggaa tgatccccgc ttctttgcgg ttttctggcg
    24181 tatagccaat gccgcgtttc agcatgtcgc cgtaatcggg gcgcatgatt ttctcgccgt
    24241 tggtaacaat ttcgccctgt tcatacgtct ccagcccaac aatcgccttc agcaattcac
    24301 tgcgccctgc ccccagcagg ccagcaatgc caagcacttc gccacgacgt agcgtaaagc
    24361 tgatatcctc cagcttgggc ttatggcgta acgcacggac ttccagtacg gcctgatcca
    24421 caatttcctg aggggcaacc ggcgcaatat caacgtgatc acgccctagc attagcgaca
    24481 caatatgatg cgtggaggtg ttttcgagca tcacatcgcc cgccacctga ccatcgcgca
    24541 taacggtggc ataggaggca atgcggcgga tttcttccat ccggtggctg acataaatca
    24601 ccgccacgcc cagtgccgac atctttttca ccgcactgat caccagttca acttctgcac
    24661 tggcaagcga gctggtgggt tcatcaagaa tgaccacgcg cggctcgccc ttcatcaccc
    24721 gtgcaatttc caccagttgc ttttgcgccg ggcttagcgt tgaaacaagt tgttcaggac
    24781 taacgtcaac gcccagcgcc tgtaagcaac gttgggcatc ctgcgccatt tgcaggtaat
    24841 caatcatgcc gttgcggcga ggccattgac caaggcagag gttttctgcc accgtcagcc
    24901 cttccaccag acttaattcc tgataaaccg cacgaacccc cagttcagcg gcacggcgag
    24961 tcaacgtagc ttcgtcacct tccagtcgcg tctcgccaat ccagatatca ccgctatccg
    25021 ggcgttcgct gccggtaagc attcgaatga gagtcgattt gcccgcgccg tttttaccta
    25081 acagcgcacg aacttcgcct ttattgagcg tgaagttaac gttatccagc gcaacgacgc
    25141 cggggtaacg cttatttcct gccaccactt ttgctaccgg gactgcctct gttgccgtga
    25201 acatagggac ctctgcgaat cagcgattag ggcagaccat caacgtgcgt tgccagccac
    25261 tgtttaccat cttccgtttt ggtatagaga tcgattggca cctgaatcac tttttcaccg
    25321 tcggcttgtt tattgataac cttcaatgtt tgcgcgaaaa cagcattgcc cattttctta
    25381 ccggaaatat ccactaccgc tttcagcacc tgattgtttt ccagctcctg agcaatttcg
    25441 gttgtcatat ccgaaccgaa aacagcaatt tttccggcct gattttgatt acgtaccgct
    25501 tttaccgcgc cgagtgtcgc accgcccgac tcccccataa tggcgttgag atccggcgtg
    25561 gagataatca gtttttcacc aacggaaatc gctttatcta aaacagtccc ttcctggtta
    25621 gcgacaattt gcgcgccggg aacgcgggat tttaatactt cttcaaatcc tttacgtcgc
    25681 tgcacacaaa cttcaaaggc ttcgcaattg atgacggcaa ttttcggctg gtcaatttta
    25741 ttggcaataa aataatcggc ggcagcgtta cccagttttt taccaaattc cagtggatcg
    25801 ccgaccagat acgccgagac atatttatcg acaccctttt gattaataca ggtgttgtag
    25861 caaatcaccg gaatacccgc ttcactggcg cgacgaacgg tacggctact gccattttca
    25921 gacactgccg ataaaataat ggcatcgaca ttacgcgcca cgagggtatc aacaaaggta
    25981 ctttctttcg aaatatcccc ctgggcgtta gtttcaatta actgtacctg tactgaagaa
    26041 tcttttgccg catcctgaac gccctgacgc actccagcgt aatatccctg ggtatcaagg
    26101 tatattgcac caatggtcat ttctttatcc gcagccctgg caaatagtgc gctacctaat
    26161 agcgtagcca ttaataatag attacgggtt gttctcattt ttttaggcat agtgcctcct
    26221 gtagggtttt tattaacaac ggcttattct aattattttg tgatgagcgg cagcgcgtgc
    26281 cgctcaaata ttacagttaa tagggtttag tgaacgtagt taaaaataaa aggcatcaca
    26341 gtggtgaata atgccgcttt cggccaggcc ggattaatcg ccgtcaattc agcgcgagca
    26401 gactcatatt gcgcaacctc cccgagtccc gcggacgcca gcatcgccac catcaggcat
    26461 ttttctttat gctgctgttg taaatcgcca tacagcgata acaggtcagg ttgtgaaaca
    26521 gcaaagaaat ccgcctcaat actggttttc gccatctctt gcgcccactg tttcatttca
    26581 ctaaacagtt gctgtgcggt ttgctgttca cccagcagtc gcagcgccat tccttgccag
    26641 aagagataat caaccggctg atcgttgtaa taactgtgga tgttaatggt gcgatcgccg
    26701 gtcgccgcca gacgtaaaca acgcatcgct tcagtttcat cgccctgcgc gttggcgcat
    26761 atcgcctgcc agaaccagat gtcgttatca gtttgccccg gtaaacggcc ttcgcttaaa
    26821 ttctccggat aatacagcgc ggcatgaagc agttcgctgg cctgctgcgg ctctctggca
    26881 tcaagatgct gccaggcgcg taataactgg ttgaggataa actgactggt gaccttccct
    26941 tccccgcctt cccacgggta gaatttacgc gtggcgagaa tgtccgccgc tttgtctgcc
    27001 tgacccgtga gatgccacaa attgagcagt tctgcggtca tgtcgtcgcg tttcagcgca
    27061 atttccagat tattttccag acgcgccagt cgtttctccg gtgtggctcc acttaactta
    27121 tccagcaaat cccgttcgaa aagcagacgt gcatcctgcg gcgcaagctg ataagcatta
    27181 tcaagataac gcgcggccag ctcataatcg tgtttcttat tccacgcatg gatcgctaac
    27241 ccgcgccagc cgtcggcaaa ctccggcgac atctctacgc aacgttgcca taaggcaatg
    27301 gctttgttgt agctacgttt gttgtagtag aagcaggcca gtaaatggcg agcaaaccag
    27361 cactcttcaa tactctccag cgccgccact tcttccagcg tattcgggaa acggacaaac
    27421 tgcgggaaga catcaatggc ttttgcgacc agttcgccac gttcggcttt cggcagcaag
    27481 ctggcttgca ggtaaagcgg cagcgtgcgc tggcagtcca gtgcgttcag catctctgcc
    27541 gccagggtgg gcattcccca gttaatcagt tgcccggcgg tcatcagagc gttaacgtcg
    27601 cgtccctgac acagaccgcg ccactgcgcg agggctgatt cgctacgacc atcgaaccag
    27661 ttcagccacc acagagtggc gttcagcgga taatcacgca gcagtttctc gcgctgcaaa
    27721 cgcgcgttgt cctgacgacc acttaacacc agcagcagat tatgcaagca aagcacttcc
    27781 tgattggttg ggcaggcacg aagactttgt tggcaaaaat ccagacccgc gtcgaagtta
    27841 tcattacgcg ccgccagtcg tgccagacca taatagccgc ctgctttact attgccgctc
    27901 cagaccgcac gccagaaatc ctcttcggct tgttgatatt gtccctgacg ttcgtaagca
    27961 ctggcgcgaa tcaaacttgc ttgtccacac tgcgggtttt tgttcagcgc atgtgcgcgt
    28021 ttcagcgcct gactggcata cgccaccgct tgcgggaaat ctgcgcggtt atattccagc
    28081 atcgccagcg ccaggttaca gcgataatcc agcgggtcca gcgccacgcc gcgcaggtag
    28141 taatcgaacg gtgaacggct ggcgtgatga tattgctcca gatgctgacc gataaaccag
    28201 gcttcatctg tactggtaat gtcttgtgct gccagtggcg ctttggcgac gtccggcaac
    28261 ggcaactcct gcggctgatg ttcctgataa ctcagtacaa tattgccgtc ggcatcggag
    28321 agttcaatgg tcagtctgtc aggattgata ccgtgcaaca cgccctggat ggcggtcgct
    28381 ggtgtcaggg ccacggcatc atcgagcaac gcgttgcatt tgccgatttc gcggatcgcc
    28441 agacggtatc cgttcaacgg agagatggca tacagccccc actcaatccc ccgctcacta
    28501 cgctggagtt ttatcaccgc atcgcgggag gcattttgca ccatgcccag agaatgataa
    28561 ggcaggaaat actgctcgaa acgcttctct tcgtaagcat caagccaggt aaaatcaggc
    28621 tggttatcgg caaaaatacc ggtcatcagt tcgatatacg ggccgttatt gtcggtcaga
    28681 ctcttatccc acgcctggcc aaattcactg tgtccccaac tccactgttt tttacctggc
    28741 gcaatatggt ggttggcaac gtgcagcaaa ccgccatctt catcgtgaca ccacgcgccg
    28801 acaaaatcgt actgcgattt ttcggccata tatgaggttg gaacaggcac atttttatag
    28861 cgagaaatgt ccactccggc ggagtagtcc actttgtagt aagtgccggt ggcgatgggg
    28921 aaagcggaga cggcccgttt gccgtgatca aacaccgcag tcacatccgg tgggaaaacg
    28981 ctctgatgcc cttcaccccc tttcactgcc gggttggccc accacaagaa atgacgcggc
    29041 gtggcgttgc cgttatagac gcggctggcg atttccagtg ccgcccggtc agggcgcagg
    29101 gtgaaacctg tcatcacctg taaaccatgc atcggctccg tttcgccgac ccacaccgtc
    29161 tgtgcaccgt cttcatgggc ttcgagggtg aaatcaacgg gcataaaggt ggtcgggcga
    29221 tggtgttgcg gccagttaaa ctcaatcccg ccggagatcc acggtcccag catccccacc
    29281 agcgcaggtt taatgacttc attgtgatag acaaaatcgc gctgtttcac tttatcccat
    29341 gcgcgatgca cccgaccgcc cagttccggc aggatcatca ctttgatgta gtcgttttcc
    29401 agccacaccg cctgctagga tttcagggtt ttctgctcgc tcagcgtatc ggtcacgccg
    29461 tagggataaa ccgcgccgga cgatccctga taaacgcgat tttccaggaa catgggatgt
    29521 atatcctgcg gcccggtttc ataggtcggg atctcaacgc gctcttgcca cacttttact
    29581 ggagtcatgg tgccctcaat attaacaaga catactgaat taaaagattt gtggcagtgt
    29641 attgaacaat ctggcaatgt tttcgcggaa taatcacgca attaactaaa caaggtttag
    29701 tgaagatgag agcctgcatt aacaatcaac agattctcca ccataacaaa tgcgtgattc
    29761 tggaactgct gtaccggcaa aagcgcgcca ataaatcaac gctggcccgg ctggcgcaaa
    29821 tttcgattcc ggcagtcagt aatattttgc aggaactgga aagcgaaaaa cgggtggtga
    29881 atattgacga tgaaagccag acgcgcgggc atagtagcgg tacatggctg attgcgccgg
    29941 aaggtgactg gacgctgtgc ctgaacgtga cgcccaccag tattgagtgt caggttgcta
    30001 atgcttgttt aagtccgaaa ggcgaatttg agtatttaca gattgatgta ccgacgccgc
    30061 aggcgctgct gtccgaaatc gaaaaatgct ggcatcgcca ccgtaaattg tggccggacc
    30121 gcaccatcaa tctggcgctg gcaatccacg ggcaggttga tccggtgacc ggcgtatcgc
    30181 aaaccatgcc gcaagcgccg tggacaacgc cagttgaggt gaagtatctg ctggaagaga
    30241 agctgggcat tcgggtgctg gtcgataatg actgcgtgat gctggcgctg gcggagaaat
    30301 ggcaaaataa ttcgcaggaa cgggatttct gcgtgatcaa cgttgattac ggcattggct
    30361 cgtcgttcgt gattaacgag caaatttatc gcggcagttt gtatggtagc ggacagattg
    30421 gtcacaccat cgttaatccg gatggcgtcg tctgcgactg tggacgttac ggcagcctgg
    30481 aaaccgtcgc ctcgttaagt gcattaaaaa aacaggcgcg ggtatggcta aaatcacaac
    30541 cggttaatac tcagcttgag cctgaaaaac tgacaaccgc gcagttaatc gctgcctggc
    30601 agagtggaga accgtggatc accagctggg ttgatcgctc tgccaatgcc attggtttga
    30661 gtctgtataa cttcctcaac atcctcaata ttaatcagat ttggttgtac ggtcgcagtt
    30721 gtgcctttgg tgagaactgg cttaatacta ttattcgcca gacaggattt aacccgttcg
    30781 accgcgacga aggaccgagc gtgaaagcga cgcaaattgg cttcgggcaa ttaagccgcg
    30841 cacaacaggt gctgggaatt ggctatttgt atgttgaggc gcagttacga cagatttgat
    30901 ggcgcgataa cgtagaaagg cttcccgaag gaagccttga tgatcataaa cgaaaaattg
    30961 cctgatgcgc tacgcttatc aggcctacac ggagattgca atatattgaa tttgcaagat
    31021 tttgtaggcc tgagaaggtg ttcatgccgc atccaacatg aacaacgagc acattgtcag
    31081 caaatcaccg tttcgcttat gcgtaaaccg ggtaacgtgc gcagatgtcg agaactttac
    31141 ctttgatgcg ctcgataacg gcttcatcat tgatgctgtc cagcacgtca cacatccagc
    31201 cagccagttc tttcgcttcg gcttctttaa agccgcgacg ggtaatcgcc ggagtaccta
    31261 cacgaatacc ggaggtcaca aacgggctct tcggatcgtt cggtacgctg tttttgttga
    31321 cggtgatgtt agcacggccc agagcggcgt ctgcttcttt accggtcagg tttttatcaa
    31381 ccagatcaac caggaacagg tggttatcag tgccgccgga aaccactttg tagccgcgct
    31441 cgaggaacac ttctaccatc gctttagcgt ttttcgcgac ctgctgctgg taagttttga
    31501 actcaggctc catcgcttct ttcagagcaa ccgctttacc ggcgattacg tgcatcaacg
    31561 gaccgccctg accaccaggg aaaacggcag agttcagttt tttgtacagc tcttcgctac
    31621 cacctttcgc caagatcagg ccgccgcgcg gacccgccag ggttttgtga gtggtggtag
    31681 taacaacgtg agcatgagga accgggttcg ggtagacgcc agcagcaacc aggcccgcaa
    31741 cgtgcgccat atcaacgaac agataagcac cgatgctgtc agcgatttca cgcattttcg
    31801 cccagtcaac cacgccggaa tatgcagaga aaccaccgat aatcattttc ggcttgtgtt
    31861 ctttggcttg tttttccaga tcggcgtaat cgatatgacc ggtagcatcg ataccgtaag
    31921 gaacgatgtt gtacagttta ccggagaagt taaccggaga accgtgagtc aggtgaccgc
    31981 catgcgccag gttcataccc agaacggtat cacctggttc cagcagcgcg gtgtagaccg
    32041 caaagttagc ctgggagccg gagtgcggct ggacgttagc gtagtcagcg ccgaacagtt
    32101 ctttcgcacg atcgatcgcc agttgttcaa cgatatcaac atactcgcaa ccgccgtagt
    32161 agcgtttgcc cggataacct tcagcatatt tgttggtcag ctgagaaccc tgcgcctgca
    32221 ttacgcgcgg gctggtgtag ttttcggagg cgatcagttc gatgtgctct tcctgacgta
    32281 ctttttcctg ctccatagcc tgccacagtt cggcatcata atcggcaatg ttcatttcac
    32341 gctttaacat ccgcatctcc tgactcagct aacaataaaa tttttggcct ttataggcgg
    32401 tcctgttgga caacggcgaa cagtataacc gaatcattgt gcgataacag gtcttgacaa
    32461 aggaatttac gcaaacgatt accttcaggc tacgcaaggc tttggagaat aaagagcttg
    32521 caaccggaaa cggatttctt ttcaggtttg tgatgcaaat ttttcacttc atcacattct
    32581 ttctgaaaaa caccaaagaa ccatttacat tgcagggcta ttttttataa gatgcatttg
    32641 agatacatca attaagatgc aaaaaaggaa gaccatatgc ttgacgctca aaccatcgct
    32701 acagtaaaag ccaccatccc tttactggtg gaaacggggc caaagttaac cgcccatttc
    32761 tacgaccgta tgtttactca taacccagaa ctcaaagaaa tttttaacat gagtaaccag
    32821 cgtaatggcg atcaacgtga agccctgttt aacgctattg ccgcctacgc cagtaatatt
    32881 gaaaacctgc ctgcgctgct gccagcggta gaaaaaatcg cgcagaagca caccagcttc
    32941 cagatcaaac cggaacagta caacatcgtc ggtgaacacc tgttggcaac gctggacgaa
    33001 atgttcagcc cggggcagga agtgctggac gcgtggggta aagcctatgg tgtactggct
    33061 aatgtattta tcaatcgcga ggcggaaatc tataacgaaa acgccagcaa agccggtggt
    33121 tgggaaggta ctcgcgattt ccgcattgtg gctaaaacac cgcgcagcgc gcttatcacc
    33181 agcttcgaac tggagccggt cgacggtggc gcagtggcag aataccgtcc ggggcaatat
    33241 ctcggcgtct ggctgaagcc ggaaggtttc ccgcatcagg aaattcgtca gtactctttg
    33301 actcgcaaac cggatggcaa aggctatcgt atcgcggtga aacgcgaaga gggtgggcag
    33361 gtatccaact ggttgcacaa tcacgccaat gttggcgatg tcgtgaaact ggtcgctccg
    33421 gcaggtgatt tctttatggc tgtcgcagat gacacaccag tgacgttaat ctctaccggt
    33481 gttggtcaaa cgccaatgct ggcaatgctc gacacgctgg caaaagcagg ccacacagca
    33541 caagtgaact ggttccatgc ggcagaaaat ggcgaagttc acgcctttgc tgatgaagtt
    33601 aaggaactgg ggcagtcact gccgcgcttt accgcgcaca cctggtatcg tcagccgagc
    33661 gaagccgatc gcgctaaagg tcagtttgat agcgaaggtc tgatggattt gagcaaactg
    33721 gaaggtgcgt tcagcgatcc gacaatgcag ttctatctct gcggcccggt tggcttcatg
    33781 cagtttaccg cgaaacagtt agtggatctg ggcgtgaagc aggaaaacat tcattacgaa
    33841 tgctttggcc cgcataaggt gctgtaattt gatgttgccg gatggaaaca tccggcaacc
    33901 cttgacgcgg tttaaattgc cgcgtcgtcc tcttcaccgg tacggatgcg aatgacccga
    33961 gccacgtcaa agacgaagat tttaccgtca ccgattttgc cggtttgcgc cgtgcgaata
    34021 atggtatcga cacaggtatc gacaatgtcg tccggtacga caatctcaat tttcactttc
    34081 ggcagaaaat ccaccatata ctccgcgccg cggtacagct cggtatggcc tttctggcga
    34141 ccaaagcctt tcacttcggt caccgtcatg ccggtaatac cgacttcggc cagtgcttcg
    34201 cggacatcgt ccagcttgaa gggttttata atcgcatcaa tctttttcat gctattcctt
    34261 gaaaaggttg cctgtctttt gatctgctaa acgtaacaca taacgccaat tcattccttg
    34321 aaatcgtttg catccagctc gtgtcgggaa agcagtttat aaaattctgt ccggttgcgc
    34381 cccgccattc tagccgcgtg ggtgacgttg cctttggtga tttgcagcag cttacgcaaa
    34441 tagttgagtt caaaatgatt acgtgcctca acaaaggttg gcagcgccgt attttcaccc
    34501 tccagcgcct gctccaccag cgcatcacta atcaccggag atgaggtcag cgccacgcac
    34561 tgttcaatca cgttgaccaa ctggcgcaca ttacccggcc agctcgcggt catcaggcgt
    34621 ttcatcgcat cggtagagaa cgcgcggaca aacggtttat gtcgctctgc cgcctggcgc
    34681 aacaggtgat ttgccagtag cggaatgtct tctgtgcgct ccgccagtgc cggaattttc
    34741 aggctgacaa cgttgaggcg gtaatagaga tcttcgcgga attccccgcg cgccatcgct
    34801 tttgacagat cacggtgagt ggcagaaata atccgcacat tgatatcaat atcgcggtta
    34861 ctgcccagcg ggcgcacttt acgctcctgc aacacgcgca gcagtttgac ctgtaacggc
    34921 gcaggcatat cgccaatttc atcgagaaat agcgttccgc cttccgccgc ctggaataaa
    34981 ccttcgcgat tgctgacagc gccagtaaac gcgccacgcg catgaccaaa cagctccgac
    35041 tccagcaatt gctcgggcaa tgcgccacag ttaatagcaa taaatggttt gctgttgcgc
    35101 gggctggcgt tgtggatagc ctgggcgaaa atctctttcc cggtgccgct ctgaccgtta
    35161 atcaaaacgc tgacgtctga ttgcgccacc agccgcgcct gttccagcaa acgcagcatc
    35221 agcgggctgc gggtgacaat tgcctcgcgc cagcgttcat cggtggctgg cgcggattgc
    35281 tccagcgcat cgtcaattgc ctgatatagc gcatctttat cgacaggctt ggtgaggaaa
    35341 ctaaaaacgc cctgctgtgt tgcagcaacg gcatcgggga tagaaccatg cgcagtaaga
    35401 ataattactg gcattcccgg ctgcactttc tggatttcag caaatagctg cataccgtcc
    35461 atttcatcca tccgcaggtc gctgataact aaatctactt tttcgcgatt cagtacccgt
    35521 aatccttcag cgccactttc cgccgtgacc acactgtagc cttcgctggt caggcgcagg
    35581 ccaagcagtt tcagcaatcc cggatcgtca tcgaccaata ataaatgcgc aggtttatgg
    35641 ctcatcagga gtgacctcat gggtggatgg cgcgggcttt tcactctcgt gcggcgtatc
    35701 cggcgagaaa tttccagccg gtttgcgggt cgagagctgg cgttcaatat cggtcaggtt
    35761 ttccagcttg cgggtggtga gttccagttg ctgctgtaga acgtgatgtt gctggcgcaa
    35821 tgtatccagc tcgctgtcgc tggactgctg gagtttactg taacgttggc gctcttccgc
    35881 cagttgcagt tgcagcgcct gaccgtcgcg ccagagttga tacagtgggc gaacctgtgc
    35941 cgggatctcg gtacttaacg cttcaatacg cgcgaccagc tggcggcgct catacggcgt
    36001 aattttggcg tcggcgagca aaatcccttg tttaaaggta ttttgccagc tgccgtcgtc
    36061 atattggcga gcttgctgac gcgactgcgc aggcattaaa cgatcagcac aatccatcgc
    36121 ccgcagccag taaagcggat tggtttcggt tgatttacct tgcagcgccc agatgtcgct
    36181 acattcagta gaaagatagt cagccagttg ataaaccgga attttttctt ctgctggcgt
    36241 atcaatggct ggcttattgt gattctgcac gcaacccagc aatgccagac atggcagccc
    36301 tgccagccac agccgtcggg gcaataatcg ttgaaaaatg tgtcgcatat tcaccagact
    36361 taaagcctat cccagtgggc gtaattgttg cagacagtct ggacatggac agcgcggaga
    36421 aaccggggcg tacatatcgt acgtgaagat ttcgagcact acccggggcc gaactgacaa
    36481 ataaaatagc ctgataggat aggctgttag cattatttcg tgtttttcga cgacggtaat
    36541 tcaatgcgga aacaaacgtc ttgcccgctc tcgtcgacca gatacagttc cccttgcata
    36601 cggcgaatac aatccctggc aatgcttaat cccagaccgc tgcccttcac cgcccctttt
    36661 cgctggtggc ttccctgaaa aaaggggtcg aagatcatgg cgcgttcctc ttgcggaatg
    36721 ggcgtgcctg tattgatgac atcaatataa acccgcgcac catgtaaact gctgcgaagg
    36781 caaatgttac cggattcagc cccgtagtgc accgcattgg agtaaagatt atccagtacg
    36841 ctcatcagca gcattggctc cgccaggcaa gctgttgctt tgagatcgac gtcggtatgc
    36901 atcattttag cccgtgcggg caggctatga gcagaaacca ctgtctccac cagcggtgct
    36961 aattcaacat tctccagttc caccgcactg tccgcctgtt tacggttgta atcaagcagt
    37021 tgttcgatca gtttttgcaa attgcagctg ctgctatcaa gaatgctcac cacctctttt
    37081 tgctctggcg taagcggccc gacaacctga tcagccagta attcagtgcc ctcgcgcata
    37141 ctcgccagtg gcgtttttaa ttcatgagat aaatgtctta aaaattgatg gcgttgggat
    37201 tccagccatg acaggcgctc acttaaccaa agaatacgtt gcccaaccga gcgtaactcg
    37261 ctcggtccac agaacgaaac gctattgccc agagaacgcc cttcccccag acggttgatc
    37321 atgcgctcga tatttttcac cggcccgata atcatccgcg tgaaaagtag caccatcacc
    37381 agactcacca gaaatagcac cagcgattgc caaccaaaat attgcccacg ttcggcgatt
    37441 tcacgctgaa gttgctgccc acgagagaac accactgtgc gcgtggcctg caccatttcg
    37501 gtattggcac tggcaaaggc ttcaagacgc gcggcggcgg cagcatcggg accactgttg
    37561 ttacactgaa gttgggccag attgttcaaa tcctgacgca atgcctggta aagtttatcg
    37621 tctggcagta cgcctgcgtg ggcatcgagc atttcgctgt aacgcttgcg ctggctttga
    37681 taaaccttcg caagcgtagg gtcgtccagc acgcaatact gacggtagct acgctccatc
    37741 tccagcgccg cgttggtcat cgcttcacta cgccgggcat caataagcgt agtgcggtta
    37801 accagcgccg cctgatcgct aagcgcattc aggctttgcc aggcttgcca tgccagcacc
    37861 aacaggggta gtagaattag caaaaatgcc agcattacca gttgtcgtaa tgagcgggga
    37921 aaaacgggcc agcgtttcaa ggtgttactc tcgtcagacg cgaatagcct gatgctaacc
    37981 gaggggaagt tcagatacaa caaagccggg aattacccgg ctttgttatg gaataaggcg
    38041 gtgcctaact cgacgtttcg cccgatggtt gatatagcta cgctgatatc agaagttgga
    38101 cggcaggcac cttgttgtgc gtcattcgaa ttttatgtag cacgtcccga aggggctgac
    38161 ataagtcggt gaatgagcca ctgcttacta ttatgcagta actgtgccaa taaagaaaat
    38221 agtttggtaa cgtactgatt atacgttgct ttgagggatt tatggcttct tcgctatttg
    38281 aatgtaaatc agccactgtg tcgctaaaaa gagacaactt aagataaact tattagatta
    38341 caataaaatc aatcatttaa gtgtcgccag aaagcgacac ggtaaaccac ccattgtcgt
    38401 gatttacaga cacaaaaaag ctcccggagt tgggagctta tgatagtggt tggtgcttaa
    38461 tgccggatgc ggcgtaaatg ccgcatccgg gctgcaaaac caatgaactg atgacttacc
    38521 ccaactgctt acgcgcattg cggaaaatgc gcatccatgg gccatcctcg ccccagtttt
    38581 ccggatgcca ggagttgctg acagtacgga aaacacgttc cgggtgcggc atcataatgg
    38641 tgactcgacc actttcagtc gtaaccgccg taataccgtt cggtgaaccg ttcgggttag
    38701 ccgggtaggt ttcagtgact ttgccgaagt tatcgacata gcgcagtgcc accagccctt
    38761 tgctttccag tgccgccaga tgcgccgcat cacgcacttc cacgcgccct tcaccatgag
    38821 agacagcaat cggcatttgc gagcccacca tcccctgcaa cagcagagac gggctttggg
    38881 ttacttcaac caggctgaaa cgcgcttcaa agcgatcgga ggtgttacgc acaaaacgtg
    38941 gccacaactc actccccggg atcagttcac gcaggttaga catcatctgg caaccgttac
    39001 atacccccag ggacagcgtt tgcggacgat ggaagaaggt tgcaaactca tcgcgtacac
    39061 ggtcattgaa caggatagac ttcgcccaac cttcaccggc acccagcaca tcaccgtagg
    39121 agaaacctcc gcacgcgacc agggcgtgaa aatcctccag tcccgtacgt ccagtcagca
    39181 ggtcgctcat atgcacgtcg atagcatcaa agcccccacg ttggaaagct gccgccattt
    39241 caacatgcga gttcacgccc tgctcacgca gtacggcaac tttcggacgt gcgccagtgg
    39301 caatatacgg tgctgccaca tcttcgttga tatcgaacga cagttttaca ttcaggcccg
    39361 gatcggcgtc gttagatttc gcctgatgct cctgatcggc acactccggg ttgtcacgca
    39421 ggcgctgcat ctgccaggta gtttctgccc accagacacg caacgtagtg cggctttcgc
    39481 tgaatacagt ctgcccgttg gcggtaatca caaaacggtc accggaaacc gcctgcccaa
    39541 cataatggac gcaatccgca agcccatgct gtgccagtac ggactcgacc gcttcacggt
    39601 cagcggcacg aacctgaatc accgcaccca gttcttcgtt aaacaacgcc gccaggcgat
    39661 cgtcacccag agaggcgata tccgcgtcaa tgccacaatg acctgcaaag gccatttccg
    39721 ccagcgttac cagcaggcct ccatcagagc ggtcgtgata cgccagcagc ttacgctgtg
    39781 caaccagcgc ctgaatcgcg tcatagaagc ctttcagttg gacaacatcg cgtacatctg
    39841 ccggtttgtc gccaagctga cgataaacct gcgccagcgc cgttgcgccc agcgcgttat
    39901 tgcctttgcc caaatcaatc agcagcagtg cgttatcttc ggtacaaagc tgcggcgtga
    39961 tggtgtgacg tacatcttcc acgcgggcaa atgcagaaat caccagcgac agcggcgacg
    40021 tcatttcgcg ctcttcgtta ccttcctgcc agcgggtttt catcgacata gagtctttac
    40081 ccaccgggat cgtcaggccc agcgccggac aaagttcttc acccacggct ttaacggctt
    40141 catacaggcc cgcatcttca ccagggtggc ctgccgccgc catccagttg gcggaaagtt
    40201 taatgcgttt gatatcgcca atctgtgttg cggcaatatt ggttaacgct tcaccgaccg
    40261 ccagacgggc agaggcggcg aaatccagca gcgcaaccgg cgcacgctcg ccaatcgcca
    40321 tcgcttcacc gtagtagctg tcgaggctgg cggtagtgac cgcgcagtta gcgaccggca
    40381 cctgccacgg ccccaccatc tgatcgcgcg ccaccatgcc ggttacgctg cggtcaccga
    40441 tggtcaccag gaaagttttc tccgccacgg tcggcagatg cagtacgcgt ttcaccgcgt
    40501 ctgcaatggt gatcccttca cggaccagcg catcgccctt cgctttcagc gtttgtacat
    40561 cgcgggtcat cttcggcgtt ttaccaagca ggacgtccag cggcagatcg atcggctgat
    40621 tatcaaaatg acgatcgtgc agagaaagat gcagttcttc ggtcgcttca ccaatcaccg
    40681 cgtagggtgc gcgctcacgc ttacacagtt cgtcaaacag cggtaattga tcggcagcaa
    40741 ccgccagcac gtagcgttcc tgggattcgt tacaccagat ttccagcggg ctcatgcccg
    40801 gttcgtcgct tagaatatcg cgcagttcaa atttaccgcc gcgtccgccg tcgctcacca
    40861 gctccggcat cgcgttagaa agaccgcccg caccgacgtc gtggataaac agaattgggt
    40921 tagcatcacc aagctgccag caacggtcga tcacttcctg gcaacgacgc tccatctccg
    40981 ggttgtcgcg ctgtacggaa gcaaagtcga ggtcggcatc agactgacca gacgccatgg
    41041 aagacgccgc gccgccgcca agaccgatgt tcattgccgg gccgccgaga acgaccagct
    41101 tcgcaccgac gttgatctcg cctttttgta cgtgatcggc gcgaatgttg ccgatcccgc
    41161 ccgccagcat gatcggtttg tgataaccgc gcagctcttc gccattgtgg ctgttcactt
    41221 tttcttcata agtacggaag tagccgttca gtgccggacg accaaattcg ttgttaaacg
    41281 ccgcaccgcc cagcgggcct tcggtcatga tgtccagcgc agtaacaatg cgctcaggct
    41341 taccgaaatc ttcttcccac ggctgttcga agccaggaat tcgcaggttg gatacggaga
    41401 aaccaaccaa accagctttc ggcttcgcgc cgcgcccggt ggcaccttca tcgcggattt
    41461 caccgccgga accggtcgcc gcgcccggcc acggagaaat cgccgtcggg tggttgtgag
    41521 tttcgacttt catcaggatg tgcgccggtt cctgatggaa attgtagcgg cccgtttcgt
    41581 ggtcagcaaa gtagcggccc acttcagaac cttccattac ggcggcgtta tctttataag
    41641 cagagagaac gtgatctggc gtggtttcga aagtattttt gatcatcctg aacagcgatt
    41701 tcggctgttg ttcaccgtcg ataatccagt cggcgttaaa aattttgtgg cggcagtgct
    41761 cagagttcgc ctgagcgaac atatacagct cgatgtcgtt cgggttacga ccaagctttg
    41821 tgaaagcatc ctgcagatag tcaatttcat cttccgccag agccaagcca agacgcaggt
    41881 tagcgtcgat cagcgcctga cggccctgcc ccagcaaatc aacgcttgtg accggagtcg
    41941 gttgatggtg ggcaaacaac tgctctgcat catctaaatc aaaaaagacc gtttccatca
    42001 tgcggtcgtg cagttcagcg gtaacctgct gccattgttc attggtcagc gtaccggctt
    42061 ctatatagta agcaacgccg cgctcaaggc ggtttacctg ttgtagcccg cagttatggg
    42121 caatatcggt cgctttcgaa gaccagggag agatggtgcc aggacgcggg gtcaccagca
    42181 ggagtttgcc ttgcggggcg tggctggcga gtgccggacc atatttcagc aggcgttcaa
    42241 gttgtgcgtg ctcatcatcg tttaacgacg cattgaggtc agcaaaatgg acatactcgg
    42301 cgtaaatatt gtgaaccggg agcctggcag cctgaaaacg tgccagcagt ttgttgattc
    42361 ggaatgccga cagtgcaggc gaaccacgca gaatttccat cataagtctc tcgtcttcta
    42421 agctttcggt gtacccaagg ggggggaaac gggcgtcatt ataaagaatc tgatgcgctg
    42481 acgaaaccgt ttgcgtggaa ataaaatcac catcgtgaat tagcaacgcg tgccgccaat
    42541 ggctgtaata agttgccatc tggcgcaggt ttacgcaaaa tgccgctcat ttatgagtaa
    42601 acctttcact attattacgt tttttcaagc tgggacgcgc acgacacaga gaattaacta
    42661 attgaaaaaa ttaaagatta attatctgtt catcggcatt ctggcactgc tgctcgcggt
    42721 cgctctctgg ccatccattc cctggtttgg taaagccgac aaccgtatcg ccgccattca
    42781 agcgcgggga gagttgcgtg tgagcaccat tcatactccc ctgacttata acgaaatcaa
    42841 cgggaaacct tttggcctag attacgaact ggcgaaacag tttgccgatt acctcggcgt
    42901 aaaactgaaa gtgaccgtgc ggcagaatat cagccagctg tttgacgacc ttgataatgg
    42961 taacgccgac ctgctggcgg caggactggt ctataacagt gagcgggtaa aaaattatca
    43021 gcctggccct acctattatt ccgtgtcaca acaactggtt tataaagtgg gtcagtatcg
    43081 cccacgtacg ctgggcaacc tgacggcgga gcaactcacc gttgcaccgg gtcatgtggt
    43141 ggttaacgat ctccagaccc tgaaagaaac aaaattcccg gaattaagct ggaaggtaga
    43201 cgacaaaaaa ggctctgcgg aattaatgga agatgtcatc gaaggaaaac tcgattacac
    43261 cattgctgat tctgtcgcca tcagcctgtt tcagcgcgtt cacccggagc tcgccgtagc
    43321 gctcgatatc accgatgaac aaccggtgac ttggtttagc ccgttagatg gcgataatac
    43381 cctttccgcc gccctgctcg acttcttcaa cgaaatgaat gaagacggta cgctggcacg
    43441 cattgaagag aaatacctgg ggcatggcga tgattttgat tacgtcgata cgcgcacatt
    43501 tttacgcgcc gtcgatgcgg tactgccgca gttaaagccc ctgtttgaga aatacgccga
    43561 agaaattgac tggcgtttgc tggccgctat tgcttatcag gaatcgcact gggatgcaca
    43621 ggccacttca ccgacgggtg tgcgcggcat gatgatgtta accaaaaata ccgcgcaaag
    43681 cctcggcatt acggatcgta ccgatgccga acagagcatc agcggtggcg tgcgttattt
    43741 gcaggatatg atgagtaaag tgccggaaag tgtgccggag aacgaacgga tctggtttgc
    43801 cctcgctgcg tacaatatgg gctatgcgca tatgctggat gcccgcgccc tgacggcaaa
    43861 aaccaaaggg aatcctgaca gttgggctga cgtaaaacag cgtctgcctt tacttagcca
    43921 gaaaccctat tacagcaagc tgacttacgg ctacgctcgt gggcatgaag cctacgctta
    43981 tgtcgaaaat attcgtaagt atcagattag cctggtgggt tatctgcaag agaaagagaa
    44041 gcaggctaca gaagcggcga tgcaactggc gcaggattat ccggcggtat cgcctacgga
    44101 gttgggcaaa gagaaatttc cttttctctc gtttctttcc cagtcgtcat caaactattt
    44161 gacccattct ccctctctgc tgttttccag gaaagggagt gaagagaaac aaaattaatc
    44221 cgtcgaggat tgcgcttttt tctgcgcttt aatttcctgg cggcgcatgc gaaagaagtc
    44281 actgagcaac gccgcgcact catccgccag tattccttcc gtaatttcca ctcggtgatt
    44341 catacccgga tgatgcagca catccattaa agatcccaca gcgccagttt tcgcgtcacg
    44401 cgcaccaaag accacgcgac caatgcgact gtggatcatc gctccggcac acattacaca
    44461 tggttcaagc gtgacataca acgtggcgtc gatcagacga taattttgca tcaccagacc
    44521 accctgccgc agggccatga tttctgcatg tgcggtggga tcatggcgac caatcgggcg
    44581 gttccagcct tcgccgatta cccgattgtt atgcactaat accgcgccga ccggcacttc
    44641 ccgctcatcc caggcacgtt tcgccagcgt cagcgcgtga cgcatccaat attcgtggct
    44701 aaattcgact tcagacaaaa agaaaactcc ggttataaaa gcgcggcgca ttatacacgg
    44761 acgctatgct ttactctatt ccagttgctg gagttcaccg cgcggggtta ctcgccaacg
    44821 atgctgacag aaataaagca gcggattgtc ctgattactg tcgctatagc cactgtacag
    44881 ccgcagcggc gtgccgattt tgcgctccag ttgtgcgacc ttttcatgtc ccagacaacg
    44941 catcgtcaat acccaaccac catagccacg ctgaatttgg ctggcgataa gattaacccg
    45001 cggcagccag ggcgtatcga aataaaccgc ttcaaccagc ggctgcggag agccggtaat
    45061 caaccagata tcagcatcgg aacttaacag gtaggtggtt aatcgctcct gaaccagcgg
    45121 aaaggcggta acattgtcgc gaaaccagcg cacgaaatcg gcctgcaacg tctgtaaacg
    45181 tgcttcgctg tgaccaaaag tgcaccccca cagaagcaga ctcatcggcc agcgtgccgc
    45241 acgacctttt atcaataacg caatggctat aatcggtaac aacggcagga caagtaacgc
    45301 attcagcggt tggcgacgca gtaaatagcg cagaaaactg ccgaacatat cctgctgatg
    45361 caatgttcca tctaagtcaa aaaacaccac acgacgctcg tgagttgcca aaccatactc
    45421 ctctggactg aaacatcctg attaattttc gctactacat agcctaacag atagatcatc
    45481 acttttccgg caacaatcga ccgcatcatg gctaactgga atttttaatt catggcaatt
    45541 agcggcaatg gaatataaaa ttcactcgcg tgtgtctcat attgcctgtt gtcgccacta
    45601 tttcatcgcg aggaaagata tgaacggctt acttcgtatc cgtcagcgtt accaagggct
    45661 tgcccagagt gataaaaaac tggcggatta tctgctgcta caacctgata cggcgcgcca
    45721 tttaagctct cagcaactgg ccaacgaagc cggagtcagt cagtccagcg tcgtgaagtt
    45781 cgcgcaaaaa ctcggctata aaggttttcc ggcgcttaag ttggcgttga gtgaagcgct
    45841 ggcaagccag ccggaatcgc cctccgtgcc cattcataac caaatccgcg gtgatgatcc
    45901 gttacggctg gtcggcgaaa aactgattaa agaaaatact gccgctatgt acgcaacgct
    45961 aaacgttaat agtgaagaga aactgcatga atgcgtaaca atgttgcgct ctgcgcggcg
    46021 gataattctg accggtattg gcgcttcggg tctggtggcg caaaactttg cctggaagct
    46081 gatgaagatt ggcttcaatg ctgccgcact gcgcgatatg catgcgctac tcgcaacagt
    46141 acaggcgtcg tcccctgacg atctgttatt agccatttcc tacaccggtg tacgacgcga
    46201 gttaaacctg gcggcagatg agatgctgcg agtgggcgga aaagtgctgg cgattaccgg
    46261 ctttactccg aatgccctgc aacagcgtgc ttctcattgc ctgtatacca ttgccgaaga
    46321 acaagcgaca aacagtgctt caatctctgc ttgtcacgct cagggaatgt taacggattt
    46381 gctgttcatt gcgctgattc agcaggatct ggaactggca ccagaacgta ttcgtcatag
    46441 tgaagcgctg gtgaaaaaac tggtctgagt aaagaatgcg cgtataatgc ccgcccggtt
    46501 tgtgttgttt tgagagtttc cttatggcgc tgttaatcac taaaaaatgc atcaattgtg
    46561 atatgtgtga aatcaaataa aaacacattt attttcatag tgatacagca atgtgttatt
    46621 gaatgtgttg ttggatttat ggtgcaaaaa aattttgcat aagttcagca acacaaagaa
    46681 caccactttc tttaacgtcc tccgcgccct aaaggacggg gctttacggc gcaaaaggcc
    46741 tgtgacatac aacattaact tttcagtcaa ccgtagcacg ttcttgtata cgccgtgtct
    46801 gcggttttat ataaccctgg ctggaaacct cttatacaaa gtcgattcgc caacatcata
    46861 gatgatcgcc accttctggc gagaaactcc tgatgcaatt agtcgcccgg cctgcgccca
    46921 ttattctggt gaaagtttgg gacggcgacc gccaattcgt ccctgtgcgc gagcagcttc
    46981 cagtcctgct tttgttcgtt caacaatcag ttcacgttcc atttcagcca gggcacccat
    47041 cacatggaag aaaaagcgtc ccattggtgt gctggtatca attgaatcag tcagactacg
    47101 aaagttgatg cctcgttcgc gcaactcctc caccagcacg acaagatgcc gcatactgcg
    47161 ccccagccga tccagcttcc agacaaccag tgtgtcgcct gccgataatg tcttgagcag
    47221 ttttttcagc cccggtcttt cggactttgt accacttatc ttgtcttcaa aaatcagctc
    47281 gcatcctgca cagttcaacg cattacgttg tagatcggta ttctggtcat ttgt
//
LOCUS       DAEEUE010000024        46118 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.024, whole genome shotgun sequence.
ACCESSION   DAEEUE010000024 DAEEUE010000000
VERSION     DAEEUE010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46118
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.024"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(19..606)
                     /gene="mog"
                     /locus_tag="KLK84_001409"
     CDS             complement(19..606)
                     /gene="mog"
                     /locus_tag="KLK84_001409"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HBE2008793.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(721..1674)
                     /gene="tal"
                     /locus_tag="KLK84_001410"
     CDS             complement(721..1674)
                     /gene="tal"
                     /locus_tag="KLK84_001410"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBE2008794.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            1953..3382
                     /locus_tag="KLK84_001411"
                     /pseudo
     CDS             1953..3382
                     /locus_tag="KLK84_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            3452..4228
                     /gene="yaaA"
                     /locus_tag="KLK84_001412"
     CDS             3452..4228
                     /gene="yaaA"
                     /locus_tag="KLK84_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBE2008795.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(4381..4677)
                     /locus_tag="KLK84_001413"
     CDS             complement(4381..4677)
                     /locus_tag="KLK84_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBE2008796.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPSPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(4891..6177)
                     /gene="thrC"
                     /locus_tag="KLK84_001414"
     CDS             complement(4891..6177)
                     /gene="thrC"
                     /locus_tag="KLK84_001414"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBE2008797.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(6178..7110)
                     /gene="thrB"
                     /locus_tag="KLK84_001415"
     CDS             complement(6178..7110)
                     /gene="thrB"
                     /locus_tag="KLK84_001415"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBE2008798.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVVSGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(7112..9574)
                     /gene="thrA"
                     /locus_tag="KLK84_001416"
     CDS             complement(7112..9574)
                     /gene="thrA"
                     /locus_tag="KLK84_001416"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HBE2008799.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(9600..9719)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(9655..9720)
                     /gene="thrL"
                     /locus_tag="KLK84_001417"
     CDS             complement(9655..9720)
                     /gene="thrL"
                     /locus_tag="KLK84_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HBE2008800.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(9934..10620)
                     /locus_tag="KLK84_001418"
     CDS             complement(9934..10620)
                     /locus_tag="KLK84_001418"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBE2008801.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTALAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(11020..11160)
                     /locus_tag="KLK84_001419"
     CDS             complement(11020..11160)
                     /locus_tag="KLK84_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2008802.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            11256..11972
                     /gene="arcA"
                     /locus_tag="KLK84_001420"
     CDS             11256..11972
                     /gene="arcA"
                     /locus_tag="KLK84_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HBE2008803.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(12031..13383)
                     /gene="creD"
                     /locus_tag="KLK84_001421"
     CDS             complement(12031..13383)
                     /gene="creD"
                     /locus_tag="KLK84_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HBE2008804.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFLLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFILALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(13441..14865)
                     /gene="creC"
                     /locus_tag="KLK84_001422"
     CDS             complement(13441..14865)
                     /gene="creC"
                     /locus_tag="KLK84_001422"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HBE2008805.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(14865..15554)
                     /gene="creB"
                     /locus_tag="KLK84_001423"
     CDS             complement(14865..15554)
                     /gene="creB"
                     /locus_tag="KLK84_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HBE2008806.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(15567..16040)
                     /gene="creA"
                     /locus_tag="KLK84_001424"
     CDS             complement(15567..16040)
                     /gene="creA"
                     /locus_tag="KLK84_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HBE2008807.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            16251..17120
                     /gene="robA"
                     /locus_tag="KLK84_001425"
     CDS             16251..17120
                     /gene="robA"
                     /locus_tag="KLK84_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HBE2008808.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(17117..17764)
                     /gene="gpmB"
                     /locus_tag="KLK84_001426"
     CDS             complement(17117..17764)
                     /gene="gpmB"
                     /locus_tag="KLK84_001426"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HBE2008809.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWVERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            17816..18336
                     /gene="yjjX"
                     /locus_tag="KLK84_001427"
                     /pseudo
     CDS             17816..18336
                     /gene="yjjX"
                     /locus_tag="KLK84_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(18421..18747)
                     /gene="trpR"
                     /locus_tag="KLK84_001428"
     CDS             complement(18421..18747)
                     /gene="trpR"
                     /locus_tag="KLK84_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HBE2008810.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(18837..20774)
                     /gene="sltY"
                     /locus_tag="KLK84_001429"
     CDS             complement(18837..20774)
                     /gene="sltY"
                     /locus_tag="KLK84_001429"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HBE2008811.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            20985..22652
                     /gene="ettA"
                     /locus_tag="KLK84_001430"
     CDS             20985..22652
                     /gene="ettA"
                     /locus_tag="KLK84_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HBE2008812.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(22759..22926)
                     /locus_tag="KLK84_001431"
     CDS             complement(22759..22926)
                     /locus_tag="KLK84_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2008813.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(23009..23695)
                     /gene="nadR"
                     /locus_tag="KLK84_001432"
                     /pseudo
     CDS             complement(23009..23695)
                     /gene="nadR"
                     /locus_tag="KLK84_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
     gene            complement(23716..25098)
                     /gene="radA"
                     /locus_tag="KLK84_001433"
     CDS             complement(23716..25098)
                     /gene="radA"
                     /locus_tag="KLK84_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBE2008814.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSGDLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(25147..26115)
                     /gene="serB"
                     /locus_tag="KLK84_001434"
     CDS             complement(25147..26115)
                     /gene="serB"
                     /locus_tag="KLK84_001434"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HBE2008815.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            26221..26865
                     /locus_tag="KLK84_001435"
     CDS             26221..26865
                     /locus_tag="KLK84_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HBE2008816.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            26893..27908
                     /gene="lplA"
                     /locus_tag="KLK84_001436"
                     /pseudo
     CDS             26893..27908
                     /gene="lplA"
                     /locus_tag="KLK84_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            27940..28203
                     /locus_tag="KLK84_001437"
     CDS             27940..28203
                     /locus_tag="KLK84_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE2008817.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(28364..29083)
                     /gene="deoD"
                     /locus_tag="KLK84_001438"
     CDS             complement(28364..29083)
                     /gene="deoD"
                     /locus_tag="KLK84_001438"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBE2008818.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(29140..30363)
                     /gene="deoB"
                     /locus_tag="KLK84_001439"
     CDS             complement(29140..30363)
                     /gene="deoB"
                     /locus_tag="KLK84_001439"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBE2008819.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(30415..31737)
                     /gene="deoA"
                     /locus_tag="KLK84_001440"
     CDS             complement(30415..31737)
                     /gene="deoA"
                     /locus_tag="KLK84_001440"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HBE2008820.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(31864..32643)
                     /gene="deoC"
                     /locus_tag="KLK84_001441"
     CDS             complement(31864..32643)
                     /gene="deoC"
                     /locus_tag="KLK84_001441"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBE2008821.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            32901..34442
                     /locus_tag="KLK84_001442"
     CDS             32901..34442
                     /locus_tag="KLK84_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HBE2008822.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            34414..35277
                     /locus_tag="KLK84_001443"
     CDS             34414..35277
                     /locus_tag="KLK84_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HBE2008823.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(35491..36273)
                     /locus_tag="KLK84_001444"
     CDS             complement(35491..36273)
                     /locus_tag="KLK84_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBE2008824.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKHHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(36270..37343)
                     /locus_tag="KLK84_001445"
     CDS             complement(36270..37343)
                     /locus_tag="KLK84_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HBE2008825.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYEPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(37465..37626)
                     /locus_tag="KLK84_001446"
     CDS             complement(37465..37626)
                     /locus_tag="KLK84_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HBE2008826.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(37743..38348)
                     /gene="osmY"
                     /locus_tag="KLK84_001447"
     CDS             complement(37743..38348)
                     /gene="osmY"
                     /locus_tag="KLK84_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HBE2008827.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(38741..40330)
                     /gene="prfC"
                     /locus_tag="KLK84_001448"
     CDS             complement(38741..40330)
                     /gene="prfC"
                     /locus_tag="KLK84_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBE2008828.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(40421..41098)
                     /gene="yjjG"
                     /locus_tag="KLK84_001449"
     CDS             complement(40421..41098)
                     /gene="yjjG"
                     /locus_tag="KLK84_001449"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HBE2008829.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(41113..41559)
                     /gene="rimI"
                     /locus_tag="KLK84_001450"
     CDS             complement(41113..41559)
                     /gene="rimI"
                     /locus_tag="KLK84_001450"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBE2008830.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHSFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(41528..41941)
                     /gene="holD"
                     /locus_tag="KLK84_001451"
     CDS             complement(41528..41941)
                     /gene="holD"
                     /locus_tag="KLK84_001451"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HBE2008831.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            42044..43075
                     /gene="rsmC"
                     /locus_tag="KLK84_001452"
     CDS             42044..43075
                     /gene="rsmC"
                     /locus_tag="KLK84_001452"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HBE2008832.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            43352..43438
                     /locus_tag="KLK84_001453"
     tRNA            43352..43438
                     /locus_tag="KLK84_001453"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43386..43388,aa:Leu,seq:cag)
     gene            43467..43553
                     /locus_tag="KLK84_001454"
     tRNA            43467..43553
                     /locus_tag="KLK84_001454"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43501..43503,aa:Leu,seq:cag)
     gene            43588..43674
                     /locus_tag="KLK84_001455"
     tRNA            43588..43674
                     /locus_tag="KLK84_001455"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43622..43624,aa:Leu,seq:cag)
     gene            complement(43713..43949)
                     /locus_tag="KLK84_001456"
     CDS             complement(43713..43949)
                     /locus_tag="KLK84_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HBE2008833.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            44090..44878
                     /gene="fhuF"
                     /locus_tag="KLK84_001457"
     CDS             44090..44878
                     /gene="fhuF"
                     /locus_tag="KLK84_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HBE2008834.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            complement(44916..45593)
                     /gene="bglJ"
                     /locus_tag="KLK84_001458"
     CDS             complement(44916..45593)
                     /gene="bglJ"
                     /locus_tag="KLK84_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HBE2008835.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLSRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(45551..>46118)
                     /locus_tag="KLK84_001459"
     CDS             complement(45551..>46118)
                     /locus_tag="KLK84_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBE2008836.1"
                     /translation="LVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQA
                     VELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILT
                     LSERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQG
                     ARELNLPSVYGDAAEWNTAELRREMSHS"
BASE COUNT        10923 a        12560 c        11617 g        11018 t
ORIGIN      
        1 ccgctcgggg ggattttttt attcgctaac gtcgcgtctt gcactcttcg gtctgaatgc
       61 agcaaccact tctggtgccg tttcaacgta tggcccttcc agcaactgaa tgcagtacgg
      121 tacgctggca aaaataccat gcaccacaac gttaccctca gcgtccttca caccttccag
      181 cgtctcttta atagacttcg gctgaccggg taagttaagg atcagcgcct gtttgcgaat
      241 cacgcccacc tgacgcgaaa ggatcgcagt tggtacaaaa tgcaggctga tctggcgcat
      301 ctgttcacca aagccaggca tctcgcggtc cgctactgcc agcgtcgcat cgggcgttac
      361 gtcacgacgc gccgggccag ttccgcccgt ggtgagcacc agatggcaac tcatttcatc
      421 caccagctca cacaacgttt gctcgatgat cgcctgctca tcggggatta agcgggtttc
      481 cagttcaaac ggcgtggtta gcgccgatgt cagccattct tccagcgctg gaatgccttt
      541 atcctgataa acgccgctgg atgcgcgatc agagatggaa actaagccaa tacgtaaagt
      601 attcatcaag atttttccgt ttaaacaatc tcgttaaaca gaatgataca ctgcgaaggg
      661 agtgacagac aggcttcaga agaggtagcg tgaccgactt cccggtcacg ctaagaatga
      721 ttacagcaga tcgccgatca ttttttccag tttttcctgg tcaatagcaa acttacggat
      781 accttccgcc agtttatcta ctgccattgg atcctggttg tgctgccaca ggaactcgga
      841 ctcagtgata cgcgccggac gcgctttcac ttcgccggtg taagacagtt tacgttcgat
      901 agccccttcg ctctccgcca gctctttcag cagtgccggt gcgatggtca gacggtcaca
      961 gcctgccagt tccagaattt cgccgatgtt acggaagctt gcgcccataa ccacggtttc
     1021 ataaccgtgc tctttgtagt actggtagat ttcagataca gaaaccacgc ccggatcttc
     1081 tgccggagcg tactctttct tatcggtatt cgctttgtac cagtcaagaa tacggccaac
     1141 aaacggcgag atcaggaaca cgcccgcctc cgcacaagca cgagcctgag cgaaggagaa
     1201 cagcagggtc aggttacagt tgataccttc tttttccagc tgttctgcag cacggatacc
     1261 ctgccaggta gaagccagtt tgatcagaat acgatcgttg ctaatacctg catcgttgta
     1321 gagtttgatc aggcgttttg ctttcgcaat tgacgcttcg gtgtcatagg aaagacgcgc
     1381 atcaacttca gttgagatac ggcccggaac cagtttcagg atttccagac caatatttac
     1441 tgccagtttg tcggtcgcgt ccacgatctg ctgcgcgcga tcgttgctct gctgtttcgc
     1501 ccaggcgaca gcatcatcaa tcaacttacg gtattccgga atctgcgctg cgttaagaat
     1561 gagagaaggg ttggttgtgg catcctgcgg ttgatacagc ttcattgccg cgatgtcccc
     1621 agtgtcggcc actacggtgg tgtactgacg aagggaggtc aatttgtccg tcatgatagt
     1681 atttctcttt aaacagcttg ttagggggat gtaaccggtc tgccctgatg atatcacgac
     1741 gcaacgtcag cgcaaccgcc gacaagacgg ttatcgccag gcgagactgt ttcggatttc
     1801 tgacactgcg ttgatatcgc ccaccatttt tatacaaaac ctcatgtatg ctacgcagaa
     1861 gttatcaagt acctcgtagc gtatatactt cttaaacaat tggtaacgtt tacacaggaa
     1921 agtcatcgcg accggcaata agagggatat gcatgccaga ttttttctcc tttattaaca
     1981 gcgtcctttg gggatcggta atgatttacc tgctcttcgg cgcaggttgt tggttcactt
     2041 ttcgcaccgg atttgtgcag tttcgctaca tccgccagtt tggcaaaagt cttaaaaata
     2101 gcattcatcc acagccaggc ggtttaacct catttcagtc attgtgtacc agtcttgcgg
     2161 cgcgcgtggg tagcggcaac ctggccggcg ttgcgctggc aattaccgcc ggtggaccag
     2221 gtgccgtctt ttggatgtgg gttgctgcgt ttatcggcat ggcgacctcg tttgccgaat
     2281 gttcccttgc acaactttat aaagaacgtg acgtcaatgg gcagtttcgt ggcggaccgg
     2341 catggtatat ggcgcgcggg ctggggatgc gctggatggg cgttctgttc gccgtctttt
     2401 tgctcatcgc ctacggcata attttcagcg gagttcaggc gaacgccgtt gcccgagccc
     2461 tgagtttttc ttttgatttt cctccgctgg tgacgggcat tattctcgct gtctttgctc
     2521 tgctggcaat cactcgcggt cttcatggcg tcgcccggct catgcagggg tttgtcccgt
     2581 tgatggcgat aatctgggta ctgaccagcc tggtgatttg cgtaataaat atcgggcaac
     2641 ttccccacgt catttggtct atttttgaga gtgcttttgg ctggcaggaa gcggcaggcg
     2701 gcgcggcggg atatacctta agccaggcga ttactaacgg ttttcagcgc agtatgtttt
     2761 ccaatgaggt gggaatggga tcgacgccaa acgcggcagc ggcagctgca tcctggcctc
     2821 cgcatccggc agcacaaggg attgtccaga tgattggcat ttttatcgac accctggtca
     2881 tctgtacggc aagcgccatg ctgatattac tggcgggtaa cggcacaacc tacatgccgc
     2941 tggaaggtat tcagcttatc cagaaggcga tgcgggtgct aatgggttcc tagggtgctg
     3001 aatttgttac cctcgtggtt attctgtttg ccttcagctc catcgttgcc aactacattt
     3061 atgccgaaaa caatctcttc tttttacgcc tgaacaaccc taaagcgatc tggtgtttgc
     3121 ggatctgcac cttcgcaacg atcatcggcg gcaccttgct aagccttccg ctgatgtggc
     3181 aactggcaga tatataatgg cctgcatggc tattaccaat ttgaccgcca ttttactgct
     3241 ctcgcctgtg gttcatacca ttgccagtga ttatctacgc cagcgtaaac tcggcgtgcg
     3301 cccggtgttt gatccgttgc gttatccgga tatcggtcgc caactttctc cggacgcgtg
     3361 ggatgatgtt tcgcaggagt aatcacaact atcgatcaac tcattctctt tttttgctaa
     3421 agtcggcata aatttcctgc aaggactgga tatgctgatt cttatttcac ctgcgaaaac
     3481 gcttgattac caaagcccgt tgaccaccac gcgctatacg ctgccggagc tgttagacaa
     3541 ttcccagcag ttgatccatg aagcgcggaa actgacgcct ccgcagatta gcacgctgat
     3601 gcgcatcagc gacaaactgg cgggtatcaa cgccgctcgc tttcatgact ggcagccaga
     3661 tttcacgccg gcgaatgccc gccaggcgat tctggcgttt aaaggtgatg tctacaccgg
     3721 cttgcaggcc gagaccttca gcgaagacga tttcgatttt gcccaacagc atttgcgaat
     3781 gctttccggc ttgtatggcg tactgcgccc gctcgattta atgcagcctt atcgtctgga
     3841 aatggggatc cgtcttgaga atgcccgagg gaaagatctg tatcaattct ggggagatat
     3901 catcaccaac aagctgaacg aggcgctcgc agcacaaggc gataatgtgg tgattaatct
     3961 ggcgtcagat gagtatttta aatcggtgaa gccgaagaaa ttgaatgccg agattatcaa
     4021 gccggtgttc ctcgatgaga agaacggcaa atttaagatc atcagcttct acgctaagaa
     4081 agcacgcggt ctgatgagtc gtttcattat tgaaaatcgg ctgaccaaac cagagcaact
     4141 gactggtttt aatagcgaag gttacttctt tgatgaagat tcctccagca atggcgaact
     4201 ggtgtttaaa cgctacgagc agcgttaagt ttgtagtcaa tgccggatgc ggcgtgaacg
     4261 ccttatccgg cctacaaaag catgcagatt caatatattg cagaattaac gtaggcctga
     4321 taagacgcgc cagcgtcgca tcaggcgctg aatgccggat tgttacccgg catttgtcat
     4381 ttagcggtga tgtttgcctg gaccatgacc gccgtgatga tcatgaggag ctttcttatg
     4441 gtggcgcggc ggtgacggcg gtccgtgtgg gtgccagcga ttgcctcgcc attcataatg
     4501 ttgtttccac cagccgtggt cgcgccagtg acctccatcc cagtaatagc cacgattatc
     4561 acgatcgcct atctgtaatt ttactgacgg gactaacgta atttccgcag cctgtgctgc
     4621 catgggagcg accagaacca gggaaagtgc gagtacgata gattgcatct ttttcacagg
     4681 ttactccttt tatcattgcc cgttatgggt cgatatccgg agattacgcg atgagaacga
     4741 cggttgttat tctccgcaat cctaatctct aagttaccgc atttattgat aatttctcct
     4801 ttttccctgt cgtttttctc cataatttct tcataaaaaa gccgggctgc ataaaagcaa
     4861 acccggcctg attgagataa tgaatagatt ttactgatga ttcatcatca atttacgcaa
     4921 cgcagaaaaa tcggcgggca gattatgtga aagcaagggt aaatcagcac gttctgccag
     4981 ctcttttggc agatccaacg tttcaccgag aatcgcttcc acgctctctt taaatttcgc
     5041 cggatgcgcg gtgccgagga acaagccata ttcgcctgga ttcaactggt cacgcagcgc
     5101 acgatacgct acggcagcgt gcggctccga ggtgtagccc agttctttta actcacgcat
     5161 tgtctgttgc gtggtttcat catccacggc tgcataaccc agctctttca gttgccagat
     5221 tttgcggcgg aacaactctt ccacacgcgg ccagttgttc ggctgactca cgtccatcgc
     5281 gttggataac gtcgcctgag tcgctttggg tgaccactga ccgtcgtgca ggaaacgtgg
     5341 cacggtatcg ttcacgttgg tcgcagcaat aaaacgtttc accggcagac cgaatgactt
     5401 cgccagcaga cccgccgtca aatcgccgaa gtttccgctt ggcaccgaga caaccagctg
     5461 gttgcgcgcc tcctgcggca gctgcgcaac agcttcaaag tagtagcaaa tctgcgccag
     5521 caaacggctg atgttaatcg agttagccga gtttaacccc agcgccactt tcagttcttc
     5581 atcatcaaac gcctgcttca ccagcgcctg acaggcatcg aaatcgccgt cgatggcaac
     5641 agtttcgata ttgccgccca acgtacagaa cagtttttct tgcagtggac tgattttgcc
     5701 tcgtggatag aggataacca ctttcacatt cggcaaaccg tagaaagcat gagccactgc
     5761 agctccggta tcaccggagg tcgcggtcag aatggtcact ggcttatcat ccgcaatatg
     5821 ggtcagcatt tgtgccataa agcgaccgcc gaaatcttta aatgccagcg ttggcccgtg
     5881 gaacaattcc agacaaccga catcgctttc aacattggcg accggagccg ggaaggcaaa
     5941 cgccgcgcgc acgcgctctt ccaggatttc ctgtgggatt tcatcaccaa taaacgccga
     6001 gaggatcttc gcactgcggg tgacaaaatc cagcttcagc atctcatcaa tttcagtcag
     6061 gctgaattcc ggcaggtcgt gcggaaaaaa cagcccctga tttttgccca acccctgggt
     6121 tacggcttgc gcaaagctga cctgctcgtt gtgatctttc agattgtaga gtttcattta
     6181 gttttccagt actcgtgcgc ccgccgtatc cagccggcaa atatgaacaa aaccttcctg
     6241 attttgcagg tagttcttac ccaaccagtc ggcaacgcgc tgggcggtat ccggcttgtc
     6301 acatagagcg aacaaggtcg ggccggagcc ggagataccg ctcactaccg cgccgatttc
     6361 cgcgaccgcc tgccgcgcct gccggaagcc aggcagtaac cgttcacggt agggttcagc
     6421 gataacatct ttcatcagct tcgcggcaag ctcaggctga cgggaatagc aggcgtgaat
     6481 gaagccagcc agatgtcgcc cgtgcgcaat gcaatcctgg cggcgatact gcgccggtaa
     6541 aatagcccgg gcttctgccg tcgagacttt aattcccgga tacgccagca cccacagcca
     6601 ctcatcaaac cctggcactt gctggctgat gatgtcgttt tcttcgatca tcaactgcat
     6661 accaccaaga aaacacggtg ccacgttgtc gtaatgaatg ctgccggaga tacgtccttc
     6721 cagctcgccc atcaaagcca gcaaacgagt gtcattaagc ggcttgccgc agtgttcatt
     6781 catcgccatc agcgccgcga ccaccgaaca ggcgctggag cctaagcccg aaccgatcgg
     6841 catattcttt tccagagtca tcgccactgg aatttgcttg ccaagctcct ggcaaaaacg
     6901 ctcccagcac tgataaacga tattttcccg tggttctgac ggcagcttat cggcaaagcg
     6961 tccgaggttg ttgagactga atgtctctgc cgcctcaacc gtgactacat ctccgagcaa
     7021 tgcaccatca acgggtgcca ccgccgcccc gagcacatca aacccgacgc tcatattggc
     7081 actggaagcc ggggcataaa ctttaaccat gtcagactcc taacttccat gagagggtac
     7141 gtagcagatc ggcaaagaca ccggcagcgg taacgtcatt gcccgcacca tatccgcgca
     7201 gcaccaacgg cagcggctga taatagtggc tataaaaggc cagggcgttt tcgccatttt
     7261 tcactttgaa cagcggatca ttcccatcca cttcggcaat cttcacgcgg cagacaccat
     7321 cttcatcaat attgccaaca tagcgcaaaa cttttccttc atcacgggcc ttcgccacgc
     7381 gcgcggcaaa gagatcgtcg agctgtgaca gattcgccat aaaagcggca acatcaccct
     7441 cggcgttaaa ctctgcgggc agcacaggtt caatttcaat atccgccagc tccagttcac
     7501 gtcccgtttc acgagcgaga atcaatagtt tacgcgccac atccatacca gaaagatcat
     7561 ctcgcgggtc cggttcggta taacccattt cccgcgccag cgtggtcgcc tcggagaaac
     7621 tcatgccttc gtctaacttg ccgaagatat aagaaagcga acctgaaaga atgccggaga
     7681 acttcaccaa ttcatcacct gcattgagca gattttgcag gttctcaata accggtaatc
     7741 cagccccaac gttggtgtca tagaggaatt tacgccgcga tttttccgcc gcataacgca
     7801 actgatgata gtaatccatc gacgaggtgt tggccttttt gttcggcgta acaacgtgga
     7861 aaccttcgcg caggaagtcg gcatattgat ccgccactgc ctggctggag gtgcagtcaa
     7921 caatgaccgg gttcagcaga tgatattctt tcacgaggcg aattaagcgc ccgagattaa
     7981 acggctcttt ggcttgtgca agttcttcct gccagttttc cagattaagg ccatgcacac
     8041 tagtgagcag agccttcgag ttggcaacgc cgcagacacg taagtcgata tgtttattct
     8101 tcagccagct ttgctgacgc ttcagttgct ccagcagcgc accgccaacg ccaccgacgc
     8161 caatcacaaa cacttcgata acctgatcgg tattgaacag catctgatga gtaacgcgca
     8221 cgccagtggt cgcatcatcg ttatttacca cgacagagat tgagcgttca gaagatccct
     8281 gagcaatggc gacaatgttg atattggcgc gggccagcgc ggcaaagaat ttcgccgaga
     8341 tcccacgcaa ggtgcgcata ccatcaccta ccaccgagat aatggccagc cgttccgtca
     8401 ccgccagcgg ctccagtaag ccttctttca gttccaggta gaactcttcc tgcattgccc
     8461 gttcagctcg cacacagtcg ctttgtggaa cgcagaaact gatactgtat tcggaagatg
     8521 attgcgtaat cagcaccacg gaaatacggg cgcgtgacat tgcagcaaag acgcgcgccg
     8581 ccatgccaac catccctttc atccccgggc cggaaacgct gaacattgcc atgttattca
     8641 gattggaaat gcccttgacc ggtaattcgt cttcatcacg gctggcacca atgagcgtac
     8701 ctggtgcttg aggatttccg gtatttttaa tcaggcaagg gatctggaac tgggcgatgg
     8761 gggtaatggt gcgggggtga agaactttag cgccgaagta ggaaagctcc atcgcttcct
     8821 ggtaggacat cgacttcaac aacctcgcat cgggcacctg acgcgggtcg caggtataga
     8881 ccccgtcaac gtccgtccaa atctcgcaac aatcggcgcg taaacaggca gccagcaccg
     8941 ccgcggagta gtcggaaccg ttacgtccaa gcaccaccag ttcgcctttt tcattaccgg
     9001 cggtgaaacc tgccatcaac accatgtgat cagccggaat gcggcttgcc gcaatacggc
     9061 gggtggactc agcaatatcg acggtagatt cgaggtaatg ccccactgcc agcagttttt
     9121 cgaccggatc gataacggta acgttgtgac cacgcgcttc taacacgccg gccataatgg
     9181 cgatcgacat tttctcgcca cggcaaatca gcgcagcgtt gatgctatcc gggcactgcc
     9241 ccaacaaact aatgccatgc aggacatgtt ttatttgggc aaattcctga tcgacgaaag
     9301 ttttcaattg cgccagcggg aaccccggct gggcggcggc gagtcccgtc aaaagttcgg
     9361 caaaaatacg ttcggcatcg ctgatattgg gtaaagcatc ctggccgcta atggtttttt
     9421 caatcatcgc caccaggtgg ttggtgattt tggcgggggc agagaggacg gtggccacct
     9481 gcccctgcct ggcattgctt tccagaatat cggcaacacg cagaaaacgt tctgcatttg
     9541 ccactgatgt accgccgaac ttcaacactc gcatggttgt tacctcgtta cctttggtcg
     9601 aaaaaaaagc ccgcactgtc aggtgcgggc ttttttctgt gtttcctgta cgcgtcagcc
     9661 cgcaccgtta cctgtggtaa tggtgatggt ggtggtaatg gtggtgctaa tgcgtttcat
     9721 ggatgttgtg tactctgtaa tttttatctg tctgtgcgct atgcctatat tggttaaagt
     9781 atttagtgac ctaagtcaat aaaattttaa tttactcacg gcaggtaacc agttcagaag
     9841 ctgctatcag acactctttt tttaatccac acagagacat attgcccgtt gcagtcagaa
     9901 tgaaaagctg aaaaatactt actaaggcgt tttttatttg gtgatatttt tttcaatatc
     9961 atgcagcaaa cggtgcaaca ttgccgtgtc tcgttgctct aaaagcccca ggcgttgctg
    10021 taaccagtcg accagtttta tgtcatctgc cactgccaga gccgtcagca atgccatgac
    10081 tcgttcgcgt aaagcttgca gttgatgttg gtctgccgtt gtgtcacttt tcgccggttg
    10141 ttgtattaat gttgctaatt gatagcaata gaccatcacc gcctgcccca gattgagcga
    10201 aggataatcc gccaccatcg gcacaccagt aagcacgtca gccaacgcta actcttcgtt
    10261 agtcagcccg gaatcttcgc gaccaaacac cagcgcggca tggctcatcc atgaagattt
    10321 ttcctctaac agcggcacca gttctactgg cgtggcgtag taatgatatt tcgcccgact
    10381 acgcgcagtg gtggcgacag tgaaatcgac atcgtgtaac gattcagcca gtgtcgggaa
    10441 aactttaata ttatcaataa tatcaccaga tccatgtgcg acccagcggg tggctggctc
    10501 caggtgtgcc tgactatcga caatccgcag ttcgctaaaa cccatcgttt tcattgcccg
    10561 cgccgctgcc ccaatatttt ctgctctggc gggtgcgacc aggataatcg ttatacgcat
    10621 attgccactc ttcttgacca aataaccgcg aacagggtga tcactgtcaa cttattacgc
    10681 ggtgcgaatt tacaaattct taacgtaagc cgcagaaaaa gccctttact tagcttaaaa
    10741 aaggctaaac tattttctga ctgcactaac ggtttaattg ttaaaaaatg ctacatatcc
    10801 ttctgtttac ttaggataat tttataaaaa ataaatctcg acaattggat tcaccacgtt
    10861 tattagttgt atgatgcaac tagttggatt attaaaataa tgtgacgaaa gctagcattt
    10921 agatacgatg atttcatcaa actgttaacg tgctacaatt gaacttgata tatgtcaacg
    10981 aagcgtagtt ttattgggtg tccggcccct cttagcctgt tatgttgctg ttaaaatggt
    11041 taggatgaca gccgtttttg acactgtcgg gtcctgaggg aaagtaccca cgaccaagct
    11101 aatgatgttg ttgacgttga tggaaagtgc atcaagaacg caattacgta ctttagtcat
    11161 gttacgccga tcatgttaat ttgcagcatg catcaggcag gtcagggact tttgtacttc
    11221 ctgtttcgat ttagttggca atttaggtag caaacatgca gaccccgcac attcttatcg
    11281 ttgaagacga gttggtaaca cgcaacacgt tgaaaagtat tttcgaagcg gaaggctatg
    11341 atgttttcga agcgacagat ggcgcggaaa tgcatcagat cctctctgaa tatgacatca
    11401 acctggtgat catggatatc aatctgccgg gtaagaacgg tcttctgtta gcgcgtgaac
    11461 tgcgcgagca ggcgaatgtt gcgttgatgt tcctgactgg ccgtgacaac gaagtcgata
    11521 aaattctcgg cctcgaaatc ggtgcagatg actacatcac caaaccgttc aacccgcgtg
    11581 aactgacgat tcgtgcacgc aacctgctgt cccgtaccat gaatctgggt actgtcagcg
    11641 aagaacgtcg tagcgttgaa agctacaagt tcaatggttg ggaactggac atcaacagcc
    11701 gttcgttgat cggccctgat ggcgagcagt acaagctgcc gcgcagcgag ttccgcgcca
    11761 tgcttcactt ctgtgaaaac ccaggcaaaa ttcagtctcg tgctgaactg ctgaagaaaa
    11821 tgaccggccg tgagctgaaa ccgcacgacc gtactgtaga cgtgacgatc cgccgtattc
    11881 gtaaacattt cgaatctacg ccggatacgc cggaaatcat cgccaccatt cacggtgaag
    11941 gttatcgctt ctgcggtgat ctggaagatt aatcggcttt accaccgtca aaaaaacggc
    12001 gctttttagc gccgttttta tttttcaacc ttatttccag atacgtaatt catcgtccgt
    12061 tgtaacttct ttactggctt tcattttcgg cagtgaaaac gcataccagt ctatattacg
    12121 agtcacaaac atcatgccgg ccagcgccac caccagcaca ctggttccca acaacagcgc
    12181 gctatcggca gagttgagca gtccccacat cactccatcc agcaacaaca gcgcgaggat
    12241 aaacaacatg ctattgcgcc agcctttcaa taccgcctgc aaataaatac cgttcattaa
    12301 cgccccaatc agactggcga ttatccatgc cacggtaaaa ccgatatgtt cagaaagcgc
    12361 cagcaagagc aaataaaaca tcaccaatga aagccccacc agcaaatatt gcattgggtg
    12421 taaacgttgc gcggtgagcg tttcaaaaac aaagaacgcc ataaaagtca gtgcaatcag
    12481 cagaatggcg tacttagtcg cccggtccgt taattggtat tgatcggctg gcgtcgtcac
    12541 tgcgacgcta aacgcgggga agttttccca gccggtatca ttgcctgaag caaaacgctc
    12601 accgagatta ttagcaaacc agctgctttg ccattgcgcc tgaaaacctg actcgctagc
    12661 ttcccgtttg gctggtagaa aatcacctaa aaaactggga tgcggccagt tgctggttaa
    12721 ggtcatttcg ctattacgcc cggcaggcac cacagaaaga tcgccggtac cgcttaaatt
    12781 cagtgccata ttcagctgca ggttctgctt acgccagtcc ccttcgggta aagggatatg
    12841 cacgccctgc ccgccttgct ctaacccggt accgggttca atggtcagtg ccgttccgtt
    12901 aacttcaggc gctttcacca caccaatacc acgcgcatcc ccgacgctaa tcacaataaa
    12961 gggcttgcct aagatgatat ttggcgcgtt gagctcgcta agacgcgaaa catcgaaatc
    13021 ggcttttagc gttaaatcac tgtgccagac ctgaccggta taaatcccta tcttgcgttc
    13081 ttccacgttc tgattgccat caaccatcaa tgactcaggt aacaaaaaat ggataaaact
    13141 tcgtttccgc tccacggttt tatcctcttc ctgcaccgta taaagctcgg tcacaggaat
    13201 agcgataagc ggcccaacga gtttttgcgg cccgctggta ctttggcgaa tcgcatcttc
    13261 cacatcgcta cggtaatcgg cacgttcgac aatcacttgc cgaatcagca ttatcggaat
    13321 caacaacagc aatactgcac caaacaaggt ggtcattttc cagaacaggg gggatttcaa
    13381 catagtcttc tcctttgcaa tggcagcagg atacgaaggc tatgtgggaa gaatttgaag
    13441 ctatgtgaag tgacggtgaa gtcgtagcga ggccagcacg ccaccttcct gcacgttgcg
    13501 cagcgtgact tcgccgttaa acaaacgggc gacctcactg acgaacgcca accccagacc
    13561 gctgcttttt tgcccatttg cacgcggcaa agagtaaaag cgttcaaaaa tacgtgacag
    13621 cgcgtagtca ggaataccac taccggtatc cagcacctta agcgtgacgt gttcctgatc
    13681 cacttcggcg cttagcgtta tgcaaccgct ctcgggagta aaatcgatgg cgttatccag
    13741 taaattcccc agcgcctgat ccagtaacgc cggttcagca gcaacattaa cctcggtggg
    13801 cataacatgc aaagtgatgt ttttttctgc caactgcacg gtgcgcgctt cgctgacgcg
    13861 gcgaaataat gccgccacat caacagcagt cagaacgact tcctgacgat tctccagtct
    13921 tgcctggcgt agtaacgttt ccaccagcgc ctgcattcgc gcattttgtg tcagaatgtt
    13981 gtcagtaaaa cgagccacta cttcaggcgg cggaccttcg cgtaaaattt ccgccgcgcc
    14041 acgaatcgcc gccagtgggc tttttagctc atgggttaac gcataaacat actgctcaat
    14101 atagtttttc ccttccagct tcacgcgcat actttccagc gcctgcgcga gtttacgcag
    14161 ctcgctacta ccgagatcgg gaagaggaac gggcttattg tcagtgacgg aatcagcata
    14221 gcgagtgagc cgggcaatgg agcggttgat ccaccaaacc atgcctgcgc caatcaccag
    14281 tgcaatcccc aacaaaatgg cgctggccca taaaattcgc cgctcgctgc gtttaatgac
    14341 cggagccatc gccgcgttcg gtttgcctac gctcaaaacg ccaataagcc gcgagccgcc
    14401 cataatcggc gcggcaacat acatcaccga actttcggga tcggcaggat tttgcaacgt
    14461 gctgcgcgca ccatactgac cacgcaacgt tagccagacg tcattccagc gcgaataatc
    14521 ctgtccaacg gctttatttg ccgaatcgaa caatactttg ccatgcgcat cggtcatata
    14581 gacatgatat tcattgcgca ctttgttaat gccaccaata ttggcgcgaa acgggcgatg
    14641 ttgtagctga ttaaacgcct gcgccagttg cccatgcgtt gggtccccag agagcaaatc
    14701 gggacgcgcc agctccgcaa gtaacgttgc cgtgtcgatc aacgtcccct ccgttgctct
    14761 tcgcacgccc ggtttaactt ctttgacaaa aatagccagt acgaaccagg ccgccaccgc
    14821 caccagtaaa aaatagccca gcagcaaccg catgccgata cgcattacaa gcccctcagg
    14881 ctatatccca tgccgcgatg agtattaatc ggtgaaagat cggggttgat ggcgcgcagc
    14941 ttggcacgca gcgttttaat gtggatatcg acggtgcgat cgtaggtgtc ctgcgcatct
    15001 tcccacacgc tatccattag ttgctggcgg gaccagacgc ggcctggcga cttgagtaac
    15061 gtcttcagca ataaaaactc ataccgcgtc agcgttaatg gcgtgtcaaa ccagctgatc
    15121 tgcgccgcgg gttcattcaa ttcaaaatga ccaatacgga tgacgggaga cggcgtcgag
    15181 aacttcttca cccgacgcag taaggtgcgc accctggcgc acacttcgcg gggtgaaaac
    15241 ggtttagcca catagtcgtc agcgccaatt tccagcccaa gcaggcgatc gacctcttca
    15301 cttcgggccg tcaggaacag tacaggtaac gccggatgga gcgccagtaa ctggcggcac
    15361 aattcaaagc cactgatatc aggcaaacca acgtcgagaa tcatgacgtc gggaacctgc
    15421 tggcgagctt tatccagcac cggcaaacct cgctcaaaga cctcgacggc aaaaccttcc
    15481 tgctgcaaca tgtagaccag cgtgtcggct atcccttgct catcttccac taaccagacc
    15541 gtttcccgtt gcataaatcg cctctgttat tgccgccacg gcatgacagg aaccgcgcta
    15601 atcgcgtttt tcggcgaacc ttctacaact ttgtcggagt aagccagata agcgagcgcg
    15661 ttgcgtttgg cgtcataaaa gcgcacgacc tgtaacgact taaagaccag ggacgtgcgt
    15721 tttttgaata ctacctcgcc ctgagctttg ccgtttttaa tacgatccga cagttcaatc
    15781 ggcccgactt gctgacaaga aatggccgca tcggaggtat cttctgccag gcccaatccc
    15841 cctttaatac caccggtttt cgcccggctc acataacagg tgacattttt cacatcggga
    15901 tcgtcaaagg cttccacaac aattttgtga tccgggccga tcattttaaa tacggtgtcg
    15961 accgaaccaa tctcttctgc atgagccaat ggccccagca ttatcaggct taaagacagg
    16021 atcaaatgct tgtatttcat attgttacca tttttatcta ttacacgagc aattagttcg
    16081 tcacggtaaa taacatattg attaagaaca aaatctcaat tcttttattt ccgtcaagcc
    16141 ctaaaacata ctctactaag aaaaaaacac tgaatgctaa aacagcaaaa aatgctatta
    16201 tccaattacc tgatgtcagg tgctcgttgt tgaaaggatg aggatatttt atggatcagg
    16261 ccggcattat tcgcgacctt ttaatctggc tggaaggtca tctggatcag cccctgtcgc
    16321 tcgacaatgt agcggcgaaa gcaggttatt ccaagtggca cttacagaga atgtttaaag
    16381 atgtcactgg ccatgctatt ggcgcgtata ttcgtgctcg tcgtttgtcg aaatcggcgg
    16441 tcgcactacg cctgactgcg cgtccgattc tggacatcgc gctgcaatac cgcttcgact
    16501 ctcaacagac atttacccgc gcattcaaga agcagtttgc ccagactcct gcactttacc
    16561 gccgttctcc tgaatggagc gcctttggta ttcgcccgcc gctacgtctg ggtgaattca
    16621 caatgccaga gcacaaattt gtcaccctgg aagatacgcc gctgattggt gttacccaga
    16681 gctactcctg ttcgctggag caaatctctg atttccgcca tgaaatgcgt tatcagttct
    16741 ggcacgattt tctcggcaac gcgccgacca ttccgccggt gctctacggc ctgaatgaaa
    16801 cgcgtccgag tcaggataaa gacgacgaac aagaggtatt ctataccacc gcgttagccc
    16861 aggatcaggc agatggctat gtactgacgg ggcatccggt gatgctgcag ggcggcgaat
    16921 atgtgatgtt tacctatgaa ggtctgggaa ccggcgtgca ggagtttatc ctgacggtat
    16981 acggaacgtg catgccaatg ctcaatctga cgcgccgtaa aggtcaggat attgagcgat
    17041 actacccggc agaagacgcc aaagcgggag atcgcccaat taatctgcgc tgtgaactgc
    17101 tgattcctat ccgccgttaa cgctgcagct catctaatgc aggggcgtcc agatgcgaga
    17161 tgtcccctgc ggtttcaacg acccagccag acgccagcca caggctttcc tgataatcca
    17221 cgcgcgaaat agaacagtta cgcagacgta agcggcgctc tacccatgct ggtaatccga
    17281 gaatcgtact caccagacat cccagtgcaa taccatggct caccagcagc ggtcggcttc
    17341 cctgcggtaa gtcccggcaa gattccagtg ccgcattgac gcgatcgctg agctcctgca
    17401 ttgactcgcc ttcaggaata cgcccgtcaa cggtgccatt gaccagctgc cgacgccagt
    17461 tctcttcttc ttcggtcaga gaatcgatat gtcttttttc cagcacaccc atgtttaatt
    17521 cacgcaggcg agaatcaaag atgatgtcac agccgcaggc ctgggcgatg atttccgccg
    17581 tacgccgggt gcgtcctaaa tcgctactga taatatgcgt aatgccaagc tctttggcac
    17641 gggttgccac ctgcatcgcc tgttgctcac ctttggcggt cagcgggctg tcagactggc
    17701 cctgaatacg tcgctcggcg ttccactgcg tttcaccgtg gcggactagg tatacctgta
    17761 acatgctttt tttccgtata ctgctgtaac gttaattttc tgagtaatgc tgattatgca
    17821 ccaagttgtc tgtgcgacca ccaatcccgc taaaattcag gccattctgc aggcatttca
    17881 cgagatcttc ggcgaaggat cctgccatat tgcatccgtt gccgtcgaga gcggtgtacc
    17941 ggaacaaccc tttggcagtg aggaaacgcg cgctggcgca cgaaatcggg tagcaaatgc
    18001 ccgccgttta cttccagagg ctgatttttg ggtggcgatt gaagctggca ttgatggcga
    18061 cagcactttc agttgggtgg tgattgaaaa cgccagccag cgcggcgagg cgcgttcggc
    18121 aactttgccg ttacctgcgg tgattctgga aaaagttcgt gaaggcgagg cgcttggtcc
    18181 cgtgagtcgc gttataccgg tattgatgag attggtcgta aggaaggggc gatcggcgtg
    18241 tttaccgccg ggaaactcac tcgcgccagc gtctatcacc aggcggtgat cctggcgctg
    18301 agtccgtttc acaatgccgt gtatcaggca ttgtaggacg gataaggcgt tcacgccgca
    18361 tccgacattc ggtgcacgat gcctgatgcg acgctgccgc gtcttatcag gcctacaaaa
    18421 tcaatcgctt ttcagcaaca cctcttccag ccactgacgc agctccacgg gcgcggcttt
    18481 caggctgtta gatccacgcg taatcgtcgc gatgcccgcg ccgagttcat tttttaactc
    18541 acgctgactc atttcgccac gcaacagctc ttcgacaata cgcacgcgag tccccaacgc
    18601 ttcgcgctca tctggcgtca gcatcaggtt taacaacggt aaatggagat cgttttggta
    18661 ggcattctta agcaggtcga caaaacgtaa ccactcctgg tgacgctgtt ctgccatcgc
    18721 tgctgaatag ggtgattgtt gggccataat atgtcgccat tgttagcggg ggaagcaaaa
    18781 tgactccccc ggttgtactc gctaaagagt acgatagcat atcataaacg tgcggatcag
    18841 taacgacgtc cccattccgt ggcgctcatc aacgtcggtt tatcccccat gaaatagcgg
    18901 tagtaagcat cataagccag cacgttcttc acataaccgc gcgtctcgga gaacggaata
    18961 ctctcgacaa atgccacagc gtcgatacgc ccggcgctgt tgccaagcca ggttcgcacc
    19021 cgtccaggtc cggcgttata agctgctgag gagaaaatac ggttattgcc aaactgctga
    19081 taaacatatt gcaggtaact ggtgccaatg ttgatgttcg tttccggatc cagcaattgc
    19141 ccagggctgc tataaccagg aatagagaac atcttcaccg tatgggtcgc tgtaccaggc
    19201 ataatctgca tcaggccgct ggcccctacc ggtgatttca ctttcggatt ccaggcgctc
    19261 tcctgacggg caatcgccat cgcatagctt tgcgggatct ctttcccact ggtgtaacgt
    19321 ttgaaaagat cgttgtaagc cagcgggaat cgctcttcca gatgatccca aagcttcccg
    19381 gcgatcgttg cctgaacgct aagatcccac cattggttat tgaaagcata ccgagccagt
    19441 tgagcctgct ctgtttttga cttgctcttc accagattgg cccactcgct acgcgcggta
    19501 ttatcgagat tccagtacat caactcgcgc acgcgcgcca tctccggccc ctgagtcagg
    19561 gtgctgtcaa cattctgcgg cgctttatca atcttcagct catactcttc accgatgcgt
    19621 tgtgctgcaa ccatcgggta gaaaccacgc tgttgtatga gttgatgcaa aatctctttc
    19681 gcttcagctt cacgtccgcg ttccagcaat aaatccgcct gccagtaacg ccattcatct
    19741 ttctcttttg cttccatcgg cagacgcgcc agccaggtat tcaggccgcg acgatcgccg
    19801 gtgccaagcg ccattcgcac acggcgttca atcaacgacg tggattgcga gcgcataatg
    19861 gcgtcatcgc gccatttcgc ctgctcatcg gtgacatcat tgcccatcaa acgccaggcg
    19921 acgatatcgc gcagctcctg aatctgatct tcattaagct gctgcgcctg ggcaagcgac
    19981 gggatcatca gccgtgcatt ttcggcatcc tgccgcgcca cactggcaaa cgccaccgcc
    20041 gccatttgac gggtaaaatc ggtcgcgccg gttgtacgcg cgaaggtcag taccgtatta
    20101 gggttgttcg ccagtgaaat gattgccgag gcgatagtct ggtaatcggc aggcatctgc
    20161 cctgccagca ctgttaccag gcctgtgtta cccgctttca tcgccagacg gatacgctct
    20221 aaatacgcca gcggatcttg tttacctgac gcacgccaga cgctaaataa cttgtcacag
    20281 gcgttaggct ggctcttgcc ggttagccac agctctttcg ccccttgcca ggcttcttca
    20341 ctctgcccgg tgttccattt cgcatagtag taattacatt gcgcttcggt agttccgggc
    20401 ttttccgggc taaaggctaa caagccacgc cagtcttcac gccgcgccag ttcattgacg
    20461 aaacgagatt gcagcgtgcg agcgggagga agcgtggggt tagcgcgaac aaagttagtg
    20521 accgtcaccg ccggttgatt catcaggtca tcggtgatct ggcggtattc caggtaggga
    20581 taaagcggat aatccttcag tccaggcatc atttgttcga ccacatccat ctgtcgatta
    20641 tcccaggcct gcttgatttg cgcgtaacgg ctacgctgct catccagtga gtcggctcgc
    20701 gccacgctgc tgaccgtcag cagacagaca ccggcagcca acagccgcca ggtaacttgt
    20761 ttggcttttt ccacaagcgc atcctctaag tgtaaataca tcaatgcagc gtcatgccgc
    20821 gtaatagatt tatgctaacc agtcattgcc gtttacgcca cgttacggac acttttttac
    20881 ttttactgcg aaggtgatcg accaactggg attaggcggg gaaacaggct acacttcgcc
    20941 tttgaaaact catcatcacg ataaacatag aggcgaagtc caacgtggct caattcgttt
    21001 ataccatgca tcgtgtcggc aaagttgttc cgccgaaacg tcatattttg aaaaacatct
    21061 ctctgagttt cttccctggg gcaaaaattg gtgtcctggg tctgaacggc gcgggtaagt
    21121 ccaccctgct gcgcattatg gcgggcattg ataaagacat cgaaggtgaa gcgcgtccgc
    21181 agccagacat caagattggt tatctgccgc aggaaccgca gctgaacccg gaacacaccg
    21241 tgcgtgagtc cattgaagaa gcggtttcag aagtggttaa cgccctgaaa cgcctggatg
    21301 aagtgtatgc gctgtacgcc gatccggatg ccgattttga caagctggcc gctgaacaag
    21361 gccgtctgga agagatcatt caggctcacg acggtcataa cctgaacgta cagctggagc
    21421 gtgcggcgga tgcgctgcgt ctgccagatt gggacgcgaa aatcgctaac ctctccggtg
    21481 gtgagcgtcg tcgcgtggcg ttgtgccgcc tgctgctgga aaaaccagac atgctgctgc
    21541 tcgacgaacc gaccaaccac ctggatgccg aatccgtggc gtggctggaa cgcttcctgc
    21601 acgacttcga aggcaccgtg gtggcgatta ctcacgaccg ttacttcctc gataacgttg
    21661 caggctggat cctcgaactg gaccgcggtg aaggtattcc gtgggaaggt aactactcct
    21721 cctggctgga gcagaaagat cagcgtctgg cgcaggaagc ttcacaagaa gcggcgcgtc
    21781 gtaagtcgat cgagaaagag ctggagtggg tacgtcaggg aactaaaggc cgccagtcga
    21841 aaggtaaagc acgtctggca cgctttgaag agctgaacag caccgaatac cagaaacgta
    21901 acgaaaccaa cgaactgttt attccacctg gaccgcgtct gggcgataaa gtgctggaag
    21961 tcagcaacct gcgtaaatcc tacggcgatc gcctgctgat tgatgacctg agcttctcga
    22021 tcccgaaagg ggcgatcgtc gggatcatcg gtccgaacgg cgcgggtaaa tcgaccctgt
    22081 tccgtatgat ctccggtcag gaacagccgg acagcggcac catcactttg ggtgaaacgg
    22141 tgaaactggc atcggttgat cagttccgtg actcaatgga taacagcaaa accgtttggg
    22201 aagaagtttc cggcgggctg gatattatga agatcggcaa caccgagatg ccaagccgcg
    22261 cctacgttgg ccgctttaac tttaaagggg ttgatcaggg taaacgtgtt ggcgaacttt
    22321 ccggtggtga gcgcggtcgt ctgcatctgg cgaagctgct gcaggttggc ggcaacatgc
    22381 tgctgctcga cgaaccaacc aacgacctgg atatcgaaac cctgcgcgcg ctggaaaacg
    22441 ccctgctgga gttcccgggc tgtgcgatgg ttatctcgca cgaccgttgg ttcctcgacc
    22501 gtatcgccac gcacatcctg gactaccagg atgaaggtaa agttgagttc ttcgaaggta
    22561 actttactga gtacgaagag tacaaaaaac gcacgctggg cgcagacgcg ctggagccga
    22621 agcgtatcaa gtacaagcgt attgcgaagt aatgcgtaaa atgccggatg cggcgcgaac
    22681 gccttatccg gcctacaaaa ccagcaattt caatacgttg taaaactgta ggcctgataa
    22741 gacgcggtaa gcgtcgcatc aggcattgag caccgaatgc cggatgcggc gtgaacacct
    22801 tttccggtct acaacagcct tagcatcctc aatttcgtgg cgggtcaccc gtgcaggttt
    22861 tttcaaacct tgtttaatct cgacagcctc ttccagagag gttttcaact catcaaaaaa
    22921 actcatttta tacctcgcct ttgagcagtt tcgtcacggt tatccttgct cccccatcat
    22981 ctcccgcacc agctccacgc agcgcaggaa acggcttcac ttcggtagga atatattccc
    23041 agtagcggaa cgggttttcg cggatctgcg caccgctgat actcataaag gtacgtttcg
    23101 gatcgaccag caccgtctcg atccccagat gttccatata ctgtggcgca tcggcttctt
    23161 ccgaggtgta gatcaggtcc ggctggatcc ccttttcagc cataaacttt ttgatgccgt
    23221 tgctccacac atcccagcca tgcggatacg gctccatgcc ctcttcgttg aaagcatgaa
    23281 tacggatatt tttctgatat ttaaaggttt gcaatagcca gcgcagacga tccggcaccg
    23341 tgggctgctg cgacatggcg ctgtcttcaa acaacgcgcg gtcgcgggta tcgtcaaaac
    23401 ccataatgat atgcagctcg tcaacctggc tacaggcgcg ctggataagg tagatatgtc
    23461 cggtatgcag cgggtagaac ttaccgaata cgacaccaat ggttttcttc tgccggggaa
    23521 attcaagccc caaaaaacgg tgcaatgcct ccagcttttg cgcgctgggg cttttaattt
    23581 tggcattcag taactggctt aaataccctt tggtcatacc gctggcatca gctacctgct
    23641 gtagcgtgca gccctgttgc ttgatggcag ttttcaggta atcaaatgac gacatatctc
    23701 cctccgtatc tctcattata agtcgtcgaa cacgctaagc gcgtcggaga gttttttaac
    23761 gccaaaaatc tgcatccctt ccggcgcttt tttcggtacg ttagccgccg gaacaatcgc
    23821 ccggcgaaaa ccgtgtttcg ccgcttcaga aatccgctcc tggccactgg gcaccgggcg
    23881 gatctcccct gccagcccga cttcaccaaa caccaccaga tcctgtggca gcggtctgtc
    23941 acgcaggctg gaaaccatcg ccagcagtaa cgctaagtcg ccgctggttt cggttacctt
    24001 cacgccgccg accacgttca caaacacatc ctgatcggcc atttgcagac caccgtgacg
    24061 gtgcaagaca gccagcagga ttgccagacg gttttgttcc agccccactg ccacgcggcg
    24121 cggattcgcc attatcgagt gatcgaccag cgcctgaatc tccaccagca acggacgcgt
    24181 tccttcccac accaccatca cggagctgcc ggaggtcact tcatctccgc gacttaagaa
    24241 aattgccgaa gggttgctga cttcacgcag cccctgttcg gtcatcgcga agacgcccag
    24301 ctcattcacc gcgccgaagc ggtttttatg gctgcgtaag gtgcgaaaac gggagtcggc
    24361 atcgccatcc agaagcaccg aacagtcgat acagtgttcc agcactttcg ggccagccag
    24421 cgaaccatct ttggttacgt gtccaaccat gacaatcgcc acaccgcgcg ttttggcgaa
    24481 acgcgtcaga taagccgccg tttcacgcac ctgcgccacg ctaccaggcg atgattgtac
    24541 atccgccata tgcatcacct ggatcgagtc aattaccatc agcttcggtt gctcttcttc
    24601 ggcaatcagg cagatctgct cgatgctggt ttccgacaac atattgagat tgtcagtcgg
    24661 caggccaagg cgatgagcgc gcattgccac ctgttgcagc gactcttcgc cggtgacata
    24721 cagcgttttc atctgctggg ccagtttgca cagcgtttgc agcaacagcg tggatttccc
    24781 cgcaccaggg ttaccgccaa tcagaatggc acttcctggc accacgccgc ctcctagtac
    24841 gcggtcgaac tctttaaatc cggtggaaaa acgcggcagc tcttcaaggc tgatatcgga
    24901 gagtttctgg acttttgcca ccccggcgct accggcatag ccgctgagac gctcgttacg
    24961 cgccaccgtt ggcgacgcag caagacgcac ctcggtgatg gtgttccagg catgacaggc
    25021 actgcactgc ccctgccagc gcggataatc ggccccgcat tcattacaaa caaaggcgcg
    25081 ttttggagct tttgccacgg tgtacctcgt taatgctgtg ccgcccgcag gatgacgggc
    25141 aggcaattac ttctgattca ggctgccaga gaggatgcag aataccccca tcaggtcagc
    25201 gtgacggatg gtgacttccg ccttttcatt cacttttggc ttggcatggt aggcaatccc
    25261 cagccctgct gctttgatca tcggcaggtc attagctcca tcgccaatcg ccacggtctg
    25321 cgccagcggg atttcatact cctgcgcgag gcgagtcaga gttttcgctt tgtactgcgc
    25381 gtctacgatg tcgccgatca cattgccggt aaatttaccg tccatgatct ccagttcatt
    25441 ggctaccacg gctgtcaggc gcagcttgtc gcgcaggtat tcagcaaaga aagtaaagcc
    25501 gccggaggca atcgccactt tccagcccag cgtttccagc ttgagcacca gctgcgttaa
    25561 gcctggcatc agcggcagat tttcacgcac ctgttgcaga atattggcgt cagcgccttt
    25621 cagcgtcgcc acgcggctgc gcaggctggc ggtaaaatcg agttcgccgc gcatcgcccg
    25681 ttcggttacc tccgccacca tctcgcccgt tccggccagc ttggcaattt catcaataca
    25741 ttcaatctgg atggcggtgg aatccatgtc catcaccagc aaacccggcg tgcgcaggtg
    25801 cgggattttc cccagcgggg cgacatccag ctgcgcttcg tgggccaggc gtgtagcccg
    25861 tgcggtgagt gaacctgcca gacgaatcac ctgataatct tccacgcacc aggcggcaac
    25921 aatcaccatc gccgcaccca gtttgctctg gtattgggtc agacgctgtt tatccagccc
    25981 acgaccatac agcagccagc cgctacgacc tgcgtggtaa tccagtggca tcacttcatc
    26041 accacttaat gaaagaggca gacccggcca taaagagaca tcttcaggca ggtcgcacca
    26101 ggtaatgtta ggcattaagg ctcctgtaaa atcgttcgaa gcagggaaaa taacgcatga
    26161 ggctaccttg tatccattgc ttctggcaac attaagtctc aaattttcaa agggtggaag
    26221 atggctcgca caaaactgaa attccggcta catcgggcag tgattgtcct gttctgtctt
    26281 gccttgttag tggcactgat gcagggggcg tcatggttta gtcaaaacca ccagcgacag
    26341 cgtaatccac agctggaaga actggcccgc accctggcgc gtcaggtgac gctgaacgtt
    26401 gcaccgctga tgcgtaccga ctcaccggat gaaaaacgca ttcaggcgat cctcgatcag
    26461 ttaacagatg aaagccgtat cctcgacgcg ggtgtgtatg acgaacaagg cgatcttatc
    26521 gcacgttctg gcgaaagcgt cgaagtgcgc gatcggctgg cgctcgacgg taaaaaagca
    26581 ggcggctatt ttaaccagca gattgtcgag ccaattgcgg gtaaaaacgg accgctcggc
    26641 tatctgcgcc tgacactcga cacccatacg ctcgccaccg aagcccaaca ggtggataac
    26701 accactaaca ttttacgcct gatgttgctg ctctcactgg caatcggcgt agtgctgacc
    26761 cgcacgctgc tacagggtaa acgcacccgc tggcagcaat cgcccttcct gttaaccgcc
    26821 agcaaaccgg tgccggaaga ggaagaaagc gagaaaaaag agtgacccat tactacaaga
    26881 aaggaaatcg ttatgtccac attacgcctg ctcatctctg actcttacga cccgtggttt
    26941 aacctggcgg tggaagagtg tatttttcgc caaatgcccg ccacgcagcg cgttctgttt
    27001 ctctggcgca atgccgacac ggtagtaatt ggtcgcgcgc agaacccgtg gaaagagtgt
    27061 aatacccggc ggatggaaga agataacgtc cgcctggcgc ggcgcagtgg cggtggcggc
    27121 gcggtgttcc acgatctcgg caatacctgc tttaccttta tggctggcaa gccggagtac
    27181 gataaaactc tctccacgtc gattgtgctc aatgcgctga acgcgctcgg cgtcagcgcc
    27241 gaagcgtccg gacgtaacga tctggtggtg aaaaccgtcg aaggcgaccg caaagtctca
    27301 ggctcggcct atcgcgaaac caaagatcgc ggcttccacc acggcacctt gctactcaat
    27361 gccgacctca gccgcctggc aaactatctc aatccggata aaaagaaact ggcggcgaaa
    27421 ggcattactt cagtgcgttc ccgcgtgacc aacctcaccg agctgctgcc ggggctcacc
    27481 atgagcaggt ttgcgaggcc ataaccgagg cctttttcgc ccattatggc gagcacgtgg
    27541 aagcggaaat catctccccg gacaaaacgc cagacttgcc aaacttcgcc gaaacctttg
    27601 cccgtcagag tagctgagag tggaacttcg gtcaggctcc ggcattctcg catctgctgg
    27661 atgaacgctt tagctggggc ggcgtggaac tgcatttcga cgttgaaaaa ggccatatca
    27721 cccgcgccca ggtatttacc gacagcctca acctcgcgcc gctggaagcc ctcgccgggc
    27781 gactgcaagg ctgcctgtac cgcgcggata tgctgcaaca agagtgcgaa gcgctgttgg
    27841 ttgacttccc ggaccaggaa aaagagctac gggagttgtc gacgtggata gcgggggcgg
    27901 taaggtaact cgctgattaa aatatccaag gaaggaaata tgatcgatct ggaaaaccag
    27961 gaacgggaga ttatcaattt aatgttcagc cagcgtattt cctggctagc cgccgtgcgt
    28021 atcaggcata aattatcgct tgctgaagta agtaaaatgt taggtatctc gattaattct
    28081 ctaaaacaga tcgaaaagac ggaacggctt agctcaaaca tcaaaagcaa aatggcagaa
    28141 atttatggtt gtccaccaga gttgctgata tgcccttcct ggatgacggc tgaacataaa
    28201 tgaatagatt cgtgaataga tacttgaatg ttttttattt tctatccata tgaaaaataa
    28261 tagtaaatct ataaatcagt tgctattgat gaatagatac taaaaagccg gagcagtccc
    28321 ccactccggc ttaacacgac aatcgccttt cagcgaaaca caattactct ttatcgccca
    28381 gcagaacgga ttccagtgcg attttgatca tgtcgttgaa ggtggtctga cgctcagcgg
    28441 cagtggtctg ctcgtgagtg cggatgtggt cagacacggt gcagatggtc agggctttcg
    28501 cgccaaactc tgcagccacg ccgtagatac cagccgcttc catttccacg ccgaggatgc
    28561 cgtatttttc catcacgtcg aacatttcgc cgtccggaga gtagaacagg tcagcggaga
    28621 acaggttacc cacgcgagca tcaacgccca gtgctttagc tgcatctact gcgttacgca
    28681 ccatgtcaaa gtcagcgata gcggcaaagt catggtcttt aaaacggatg cggttaactt
    28741 tggaatcggt gcaggccccc ataccgataa cgacgtcgcg cagttttacg tgcggcagaa
    28801 ctgcgccaca ggaaccaacg cggataattt tcttcacgcc gaaatcggtg atcagttctt
    28861 tggtgtagat ggagcaggac gggataccca taccgtgacc cattacggaa attttgcggc
    28921 ctttgtaagt accagtgaag cccagcatac cgcgaacgtt gttcacttca cgggcatctt
    28981 caaggaaagt ttcagcaata tacttcgcac gcagcgggtc gcctggcatc aaaactacgt
    29041 cagcgaaatc gcccatttct gcattaatgt gtggggtagc cattgtttta tcctttttgt
    29101 gacataacaa aggcggagtc aacgctccgc ccaaatccat cagaacatgg ctttgccata
    29161 ttccatatca gaagtaccaa aatattttgc cagagtctgg ccgatatccg cgaaggtttc
    29221 acggtgaccc agtgagcccg gttttacttt cgggccatat accagtaccg gaatgtgttc
    29281 acgcgtgtgg tcagtaccgg tccaggtcgg atcgcaaccg tggtcagcgg tgaggatcag
    29341 gatgtcgtca tcgcgcagca gagacatcag ctccggcaga cggcggtcga acagttccag
    29401 acccgcagca taaccggcga cgtcgcggcg gtggccccag gaagagtcga agtcaacgaa
    29461 gttggtgaag acgatggtgt tatcacccgc ttctttcatc tctttgatgg tggcgtcaaa
    29521 cagcgcgtcc aagccagtcg ctttcacttt tttggtgata ccgcagttgg cgtagatgtc
    29581 tgcaatttta ccgacagaaa ccacctggcc gtgtttttca tcaaccagtt tctgcagcac
    29641 ggtcggtgct ggcggctcaa cagccaagtc gtgacggtta ccggtacgct ggaagttacc
    29701 agctttgtca ccgataaacg gacgagcgat aacacgaccg atattgtagc cgccgttggt
    29761 cagctcttca cgggcgattt cgcacagttc gtagagttta tccagaccga aagtttcttc
    29821 atggcaggca atctggaaca cggagtcagc ggaggtatag aaaatcggct tgccggtttt
    29881 catgtgctct tcgcccagtt gatccagaat gaccgtaccg gaagagtggc agttaccgag
    29941 gtaacccggc agattagcgc gttcgaccag tttatccagc agctcttgcg ggaagctgtt
    30001 ttcgtgatcg gagaaatatc cccactcaaa cagaaccggg acaccggcga tttcccagtg
    30061 accagacggg gtatctttac cggatgacat ttcgtgcgcc catgcgtacg cgccgataac
    30121 ttcagcgttg ccgtccattc ccgccggaat gaaaccggta gaaccttcgt gtgctttcgc
    30181 cagccccaga cgggtcagat ttggcagatt gagcggccct ttacgaccat tatcagcttc
    30241 gcctttggca caagcttctg cgatatgacc cagggtgtca gccccgacgt caccaaagcg
    30301 ttctgcatct tctgtagcgc cgatgccgaa tgagtccagc accataataa atgcacgttt
    30361 catatgttct ccgtactcag tgcttcaaat ttaaatgatc agatcagtat accgttattc
    30421 gctgatacgg cgatagacag ttggtgtgct ttccggtgcg ttatcggcaa gtttaattgc
    30481 cgctttcacc gctttcgccg cttcctgcca gctgttttcg tctttcgcga ggataaccgc
    30541 cagcggacgc tgaccgtcta cctggtcgcc cagacgcgcc atatcagtaa agccgacgct
    30601 gtaatcgatg gtgtcagatg cctgacgacg accgccgccc attgcaacca ctgccatccc
    30661 cagcgcgcgg gtatccattt cactgacaaa accttcggta tcagcataga ctgctttcgt
    30721 cagcatcgct gtcggcagat acttcgcgta gttctcaacg aagtcggtcg ggcctttttg
    30781 tgccgctacc atacgaccaa agacttctgc cgctttaccg ttgtccagca ccgcctgcaa
    30841 tttcgcgcgc gcttcggcgt catctttcgc cagtttgccg gagatcagca tctccacgca
    30901 cagcgccatc gtgacatcaa acagacgcgg gttacgatac tcacccgtca ggaactgcac
    30961 cgcttcacga acttcaaccg cgttacctgc actggaggcc agtacctgat tcatgtcggt
    31021 gagcagcgcg gtggtgcgca cgccagcgcc gttagccacg ccaacaatcg cttcggcaag
    31081 ggcttcagag agttcgtagg tcggcataaa cgcgccgcta cccactttca cgtccatcac
    31141 cagcgcgtcc agaccttccg ccagtttctt cgccaggatc gaggcagtga tcagcgggat
    31201 ggagtccacg gttgcggtaa tatcacgggt cgcgtagaaa cgtttatcag ccggagccag
    31261 tgaactggtc tgaccgataa tcgccacgcc gacgtcttta ataatttcgc ggaaacggtt
    31321 gtcatccggg aaaatgtcga agccagggat ggattccagt ttgtcgagcg taccgccagt
    31381 atgaccgagg ccgcgaccgg agatcatcgg aatatagccg ccgcaggctg cgaccatcgg
    31441 ccccaacatc agcgaagtga catcgccgac gccgccggtc gagtgtttat caacaatcgg
    31501 gccattcaga tgcaggcttt tccagtcgag aacggttcct gaatctcgca tcgccatggt
    31561 cagcgagaca cgctcaggca ttgtcatatc gtggaagaaa atggtcatcg cgagggcggc
    31621 aatctgccct tcggaaatag tgttgtcgcg aataccgttg ataaagaaac gaatttcttc
    31681 atcgctcagc gcatgaccat cacgtttttt acgaataatt tcttgtgcga gaaacaaggt
    31741 aacctcccga ggaaaagagt atgaggcggc ttttgccgga tgcgtctacg ccttatccgg
    31801 cctacgggta gatacgtctc gtaggcctga taagcgcagc gcatcaggcg taaagcatct
    31861 tacttagtag ctgctggcgc tcttaccgtc gccgtggccc agcgctttca gcaggcttgc
    31921 cagcaggctg gaagcaccaa agcggtagtg acgcgcatca gcccagtcag caccgaacag
    31981 ttcatctgcg atggcgagat atttctgcgc atcttccgca gtacgcacgc cgcccgccgg
    32041 tttgaaacca acggtttttt ctacgcccat atcacggatc acttccatca tgatgcgagc
    32101 gctttccggc gtcgcgttca cagccacttt accggtagag gttttgatga agtccgcacc
    32161 cgctttgatg gagatttcag acgctttacg gatcagcgct tcgtctttca gttcgccggt
    32221 ttcgatgatc actttcagca gtacattcgc tgccgcgcaa gcctctttac aggctttcac
    32281 caggtcaaaa ccaacctgct cgtcacccgc catcagcgcg cggtacggga acaccacgtc
    32341 aacttcatca gcaccgtagg cgattgccgc acgggtttct gccagcgcga tttcgatgtc
    32401 gtcgttaccg tgtgggaagt tggttaccgt agcgatacgg atttccgggg tgccctgctc
    32461 tttcagagtt ttgcgagcaa tcgggataaa acgaggatag atacagatag cggcggtatt
    32521 gccgaccgga gttttggcct gatgacacag agcaattact ttctcgtcgg tgtcgtcgtc
    32581 attcagggtg gtcaggtcca tcaatttcag tgcacgcagg ctgcttgctt tcagatcagt
    32641 catttcattc tcctggcttg tcgccaataa aattcacctt gcgagtttgt tagtattcta
    32701 acatctactc cgcaacacac ttcgatacac atcacaatta aggaaatcta cttacaagtt
    32761 tgcatcactg taatgcgatc tggttcaaat aattcacttt caaatgaatg cgtcagtggt
    32821 ggcaaacgca tcaggatcaa agtgaacatc acgaaacttc ttacaatggc gcatcacctt
    32881 cggcataaaa ggaaaagaag atgcccactt ctcatgaaaa tgcactgcaa caacgttgcc
    32941 agcaaattgt caccagccca gtgcttagcc cggagcagaa gcgccatttt ctggcactgg
    33001 aagcagaaaa caatctgcct tacccacagc ttcctgccga agcccgccgc gcgctggatg
    33061 aaggtgtaat ctgcgatatg tttgaaggtc atgcgccgta caaaccgcgc tatgtcttac
    33121 ccgattacgc tcgttttctg gcgaacggtt ccgaatggct ggagctggaa ggcgcgaaag
    33181 atcttgatga cgcactctct ctgctgacca ttctttacca ccacgtaccg tcggtcacat
    33241 cgatgccggt ctacctgggg caactggatg cgttgttgca accgtatgtt agaattctaa
    33301 cacaaaacga gatcgatgtt cgaataaaac gtttctggcg ttacctcgac agaaccctgc
    33361 cagatgcctt tatgcacgcc aatatcggcc cgtctgattc gcccattacc cgcgcgatct
    33421 tacgtgcaga cgcgcaggtt tcaccgaacc tgacctttat ctacgatcct gaaatcaccc
    33481 ctgatgacct gctgctggaa gtggcgaaga acatctgtga atgtagcaaa ccgcacatcg
    33541 ccaacggtcc ggtgcatgat aaaattttca caaaaggggg ctacgggatt gtgagctgtt
    33601 acaactcact gccgctggcg ggtggtggca gcacgctggt acgccttaac ctgaaagcca
    33661 ttgccgagcg cagcgaatcg ctggatgact tctttacgcg cactctaccg cactactgcc
    33721 agcagcagat cgccatcatc gatgcgcggt gtgaattcct ctatcaacaa tcacacttct
    33781 ttgagaatag cttcctggtg aaagaagggc tgattaaccc tgaacgtttt gtgccaatgt
    33841 ttggcatgta tgggctggcg gaagcggtta acttgctgtg tgagaaagaa gggattgccg
    33901 cgcgctacgg taaagaagcc gccgcaaatg aagtgggtta tcgcatcagc gcgcaactgg
    33961 cggagtttgt cgccaatacc cccgtgaaat atggctggca aaaacgcgcc atgttacacg
    34021 cacagtcggg gatcagttcc gatatcggca ccacgccggg cgcgcgtttg ccgtatggcg
    34081 atgagccaga tccgatcacc catctgcaaa ctgtcgcccc gcatcatgct tattattatt
    34141 ccggcatcag cgacattctg acgctcgacg aaaccatcaa acgtaacccg caggcactgg
    34201 tacagctttg cctcggtgcc tttaaagccg gaatgcgtga atttaccgcc aatgtcagcg
    34261 gtaacgatct ggttcgcgtt accggttata tggtgcgttt gtcggattta gaaaaatatc
    34321 gcgccgaagg ttcacgcacc aacaccacct ggctgggcga agaagccgca cgcaacactc
    34381 gtattctgga acgccagccg cgcgtgataa gccatgaaca gcagatgcgc tttagtcagt
    34441 aagattatcc ccttctcctg cgttgacggg ccaggcagtc gtctggctct gtttttgcag
    34501 ggctgcaatc tgcgctgcaa aaactgtcac aatccgtgga cgatgggacg ttgcaatgac
    34561 tgtggggagt gcgtgccaca gtgtccgcat caggcgttgc agattgttga cggcaaagtg
    34621 gtgtggaacg ctgtggtctg cgagcaatgt gatacctgcc tgaagatgtg tccgcaacat
    34681 gccacaccaa tggcgcaatc catgagcgtg gacgaagtgc ttagccatgt gcgcaaagcg
    34741 gtgctgttta ttgaagggat aacggtaagc ggcggcgaag ccacgaccca actgccgttt
    34801 gtagtggcgc tgtttactgc tatcaaaaac gatccgcaac tgcgccatct cacctgtctg
    34861 gtggacagta acggtatgtt gagcgaaacc ggctgggaaa aattactccc ggtgtgtgac
    34921 ggcgcaatgc tcgatctcaa agcatggggt agcgaatgtc atcaacaatt aaccggacgc
    34981 gataatcaac agattaagcg cagcatctat ttgctggcag agcgcggcaa gctggcggaa
    35041 ctgcgtttgc tgatgatccc aggccaggtg gattatttgc aacacatcga agaactggcg
    35101 gcgtttatca agggacttgg tgatgttccg gttcgcctga acgcgtttca tgcccacggc
    35161 gtgtatggcg aggcgcaaag ctgggcgagc gccacgccgg aagacgttga gccgttggct
    35221 gatgcattaa aggtgcgcgg ggtgagccgg ttgatatttc cggcgctcta tttgtgacat
    35281 gccggatacg gcctacggtt cggcacagac ttgtaggcct gataagacgc gtcaagcgtc
    35341 gcatcaggca ttgtgcgcca actgccggat gcggcgtgaa cgccttatcc ggcctacggt
    35401 tcggcacaaa cctgtaggca tgataagacg cgttaagcgt cgcatcaggc attatgcgcc
    35461 gactgccgga tgctgcctac gaagggcaaa ctacccacga acgttaaaca ccgcatacgt
    35521 gttattaagc aacacttcgg caatctcatc cgcgggttcc gggcgtaact cgcaaagcac
    35581 ggcaaacaca cgggcagcct gttccgggcg gttaggctgc ccctgaaaac cgttgagcgg
    35641 catatccggc gcatcggttt ccagcaacaa cgatgccagc ggtaattttg cgatgacatc
    35701 gcgggtttta ctggcgcgtg gataggtgat agtaccgcct acgccgattt tgtagcccag
    35761 ctgtacaaac cgttcagcct gttgctggct gccagaaaat ccgtggacaa caccagcacg
    35821 cggcaaatcg tggtgtttaa gatgcatcgc cagtttgtcg tgcgtgcgcc gtgaatgcag
    35881 gatcaccggc agatcgtagc gtttcgccag tttcagttgt tcgtcgagta accactgctg
    35941 cctctcaaat tgcggatcgt cgccgaagag atccagaccg atctccccca ccgccaccac
    36001 cttcgccgga cgcctttcca gcgcctgctg tagctgctca agagacacat cgctatgttt
    36061 ttccaacata ccaggatgca agcccaatgc ggcatacagc ggttgataat tttccgctaa
    36121 cgcctgcacg cgggcaaaat tttccgcctc tgtcgccgga acaatgatct tgcctacgcc
    36181 cgcttgtgcc gcgcgttgca ggctggcctc ttcatcgcca ataaaaggcg ggaaatcaaa
    36241 atggcagtga gtgtcgataa aacggcaaat caagccagat cctcattgtt aaatgtcggg
    36301 tcgttcgcct gcggagcgtc gctcacttcg gcaaccagcg tatcgttggc gactggcgca
    36361 ggcggaatga caatcgattc cggcgtcacc actggcacca gatggcgggt aagcggcgct
    36421 ttttcagtta atagcttgcc aaccgtggcg aggaaataac gaccgcataa acgcccaagt
    36481 ttatagtctt cacgcagcgc cggaatccga ctgccaagcg cgatactatg taatggcttc
    36541 ggcggataaa tttcggatat ccgcagcttg cccggtggtt tctcaataaa ttgctgaatg
    36601 tcacgatagc tggtttcatg atgctgcacc agattgacca gcggctgcag gctactgtca
    36661 cccagccagc gttccatgcg tttgaaccac tgcggcgtgt agtacatttg tgacggcaca
    36721 gtgcggatga cgaccaacgt tttagcgccc tgccttgccg cctctttaac cggaatcgca
    36781 tcactgatcc cgccatccag gtagttaatg ccttccagcg acactccgct acgataaaag
    36841 ccaggtatcg cactggaggc gcgaatcaca tccagccagt tttgtttggt tggtaaaaag
    36901 taattcggct cgtagtcatc ctgacgacag gcgcacatat aaaacgattt gccgctgtca
    36961 aacaaccgcg cggcggtatc catttgcaac ggcatctggc ttgcggtggc ctccaccagc
    37021 cagtcgagat cgataagatt tcctccacgg acaaagcgca atggatcgaa aaattcgcgt
    37081 tttgtggtat agcgcatgat gactttgcgc gcgtaaccgg gctgattgca gataaacgcc
    37141 gagaggttct gcgccccggc agatgtgccg agataaagat cgaaaggatt aaactgcgcg
    37201 cgcataaact catccagcac gccagccgtg aaaattccac gctgtccgcc gccttcgcac
    37261 accagagcta ttcgtccagg ctggaacggc cttagcgaca acggcgcaat attaccaagc
    37321 gttacaggta ttcgctgccc cacttctgcc ttcctgtttt tgttgtgaaa gaaagacaaa
    37381 gtaacgcaat ttattattcg ctaaaaccgc aaaagccagt ccgctggact ggctttggat
    37441 aaaattttct taatggtctg aaatctaggg tcgttttcgg cccatgaaca aactcactag
    37501 gaacagaata atcccgacga caaagacaat tttagctgcg cctgcagcgg taccggccag
    37561 accaccaaac ccaagtgcgg cggcgattaa cgcgataacc agaaatatga tgccccaacg
    37621 aaacataagc gtctccttta ccatagttaa tgtcaccgct tgatatgagc gtgaaaatca
    37681 ctcaatgcga tatttttagt gtggtgcaca tcgcgcctcc cgacaaagtt cgggaggacg
    37741 aattacttag ttttcagatc atttttaacg cttttcacac catctaccgc tttggcgata
    37801 ctttcagcac ggtcactttg tgcctgagaa tctacggtac cggagagctg aaccacgcca
    37861 tcggtggttt caactttcac tttacgtgaa gggacgatat cgtccgccag cagtttggct
    37921 ttgatttcac tggtggtggc ggtgtcaccc gcgtagccct tcaccgagcc ttctttagcg
    37981 tcgcgaacgt gcagtttgtc gctgacagag gtcacgcctt caacgccttt cgccactttc
    38041 actgcctctt cggcctgggc ctgactttca acgaaaccgc tcagggtcac gactttttga
    38101 tcggttttga cagagatatc ggtgctcttg atgttgtcat gatccaccag cgccgccttc
    38161 actttcgcgg tgatggcgct gtcatccatg aaattaccga ctttattcat agagctatcg
    38221 actttttgcc ctgcgctttc attggtagtc tgcgcgttgt tttccgcgta ggcagagccg
    38281 gtcgcgacgg cagaggtcaa cattacagcc agcagagttt tcgaaatctt cagtcttgtc
    38341 atagtcatcg atttattcct gtatgtttgc tcgtaatttg agcctagcaa cacgaggttg
    38401 cattgctgaa tagggagaga cttcaccctc tacagaagtc aatggtcgcc atcacaaaag
    38461 cgatgggtga tgaataacga ccattacagc ctctgaatca gttattaata tcggtagaat
    38521 gacaatcgac ggctttagat actgatatct acgcattgaa cggtatttaa cgccatcaga
    38581 aatgtcatca ctttgttaaa tatagatcac aattttgaaa ccgctcggga tatcagcgaa
    38641 aacataagca aaagtgaatg ttttaagaac attccgtaag cggctaataa ggaagggaaa
    38701 ttgacagggc gcagcggcta ccgcgccctg gaggcaagaa ttaatgctcg cgggtctggt
    38761 ggaactgaac gtccggataa cgttcctgtg ccaggcgcag gttgaccatg ctggtagcga
    38821 tgtaagcgag gttatcgccg ccatcaagcg ccagttggct ttcgttctta cgcttgaact
    38881 cttcgaattt cttcgcgtct gcacattcta cccagcgggc agtggcaacg ttgactgact
    38941 catacactgc ttcaacgttg tattcgctct tcaggcgcgc taccaccaca tcaaactgca
    39001 gcacaccaac cgcaccaacg atcagatcgt tgttggagat tggacggaac acctgcaccg
    39061 cgccctcttc ggaaagctgt accagccctt tgagcagctg tttttgcttc agcggatctt
    39121 tcaggcggat acgacggaac agttctggtg cgaagttcgg aataccggtg aacttcatca
    39181 tctcaccctg ggtaaaggtg tcgccgatct gaatggtgcc gtggttgtgc aggccgagga
    39241 tatcgcccgg atacgcttct tcaacgtgcg aacggtcacc cgccataaag gtcagcgcgt
    39301 cggagatcac cacatctttc gcagtgcgca cctggcgcag tttcatgcct ttttcatatt
    39361 taccggacac cacacgcata aacgccacgc ggtcgcggtg tttcggatcc atgttggcct
    39421 gaattttaaa tacgaagccg gtaaatttat cttcgctcgc ttctacggta cgggtatcgg
    39481 tctgacgcgg catcggcgca ggcgcccact ccaccaggcc attcagcata tgatcaacgc
    39541 cgaagttacc cagcgcagta ccgaagaata ccggagtgat ttcgcccgca aggaacagct
    39601 ctttgtcgaa ctcgttagac gcgcctttaa ccagttccag ttcgtcacgc agctgctgtg
    39661 ccagatcttc accaaccgca gcatcgagat ccgggttatt cagcccttta acaatgcgga
    39721 cttcctgaat ggtgtgccct ttaccgctct gatagagata ggtttcatct ttataaaggt
    39781 ggtaaacgcc tttaaacagc ttgccgcagc caattggcca agtgatcggt gcgcagccaa
    39841 ttttcagctc gttctcaact tcatcgagca gttccatcgg gtcgcggata tcacggtcaa
    39901 gtttgttcat aaaggtgagg atcggcgtgt cgcgcagacg ggtaacttcc atcagcttac
    39961 gggtacgatc ttcaacacct tttgcggcgt cgataaccat caggcagcag tccaccgccg
    40021 tcagggtacg ataggtatct tccgagaagt cttcgtgccc cggggtgtcg agcaggttaa
    40081 ccaggcaatc gtgatacgga aactgcatca cagacgtagt aatggagatc ccacgctgct
    40141 tttccatctc catccagtcc gacttagcgt gctggttgga accacggcct tttactgtac
    40201 cggcggtctg aatggcctgt ccgaacagca gcaccttctc ggtgatggta gttttaccgg
    40261 cgtccgggtg agaaataatg gcaaaagtgc ggcgcttcgc cacctctttc aaataaggag
    40321 acaacgtcat aattctttct tcttgagtaa gcggcatcgc gccgcgcttg ttgaaaacga
    40381 aaaattgcgg ctattttacc catcaacggg ggggaggcaa tcagtgttta cacaggagct
    40441 gctccagttc gtgcaacgaa gaaacggtcc aggtgggcgc gatgccttct ggttgctcgc
    40501 gattgtgcgc attcagccag caggtcgcaa gcccggcgtt gatgccaccg agaatatcgg
    40561 actcggcagt gtcgccaacc atcagcacgc gtgaacggtc aggattgccc gcctgttcca
    40621 gcgcataatc gaaaattttc ttattcggtt tggcaacgcc aacttcttcg gaaatcacca
    40681 gcaaatcgaa gtaatcacgc aggcccgtcc gttccagacg cacttgctgc aaggcgctaa
    40741 aaccgttggt gatgatgccg attttagcgt tgctacgaat ggcgttaagc agagaaaccg
    40801 cgcccggcaa cggcgtacag atttccgcca tcgcattaat aaaggcatcg ttaagcaagc
    40861 ctgcttcaac tttcagccgt tcggcccagc tctcgaaacg cccgtgctga agctgtaatg
    40921 aagtgatcgc gccgttttga taatccaccc acagtggctt gttaacggcc tgatagtcct
    40981 gaaaatcttc agcggtaaag gtgacgctgt aatcaagaaa catccgctgc aggccggtga
    41041 atgagtcaaa ggtaaacagc gtttcatcgg catcaaagaa aatccagtcc cacttcatta
    41101 ttccaccttg tcttacatac tgattggcaa cgccatgatg atggcgtctt cgcgaccgtc
    41161 cgtggtgggg tagtaattgc ggcgaatcgt cgcctcgtta aagcctaaac tttcgtacag
    41221 ggcaatggcg gcagcatttg aggcacggac ttccagccat agtgtcgcca cgccgcgttt
    41281 ttccagttcg tcgatcagat gttccagcag cgccctaccc agcccctgac gctgatagtc
    41341 gggatcgacc gcaatattga acaatgtagc ttcatccagc accacttgcg taatcgcaaa
    41401 cgccgccatt ttgccgtttt gcgttaactg aaagttgaga taacgctcgc cctggttgct
    41461 ggcaaaggtt ttttcactcc acggaaagga gtgggcgcgt tgttcaatgt ggtaagccgc
    41521 tggtaaatca gtcgtttcga gggaagaaat cgtgttcata tgtgcaaatt tgttgccata
    41581 acgcggcgcg tgccgttggg tttgcccgta attcggtgag cgccggtgat gccacctgag
    41641 cgccttccaa tgatagcggt tcgtcagtac ccaaccgcca gctgttgcag cgactgcctt
    41701 gcggcagcat cgcgattttt tctggcgtca gttgcagcac ttgatcgggg ctgacggtta
    41761 atgcgcgcag aacatcgctc actaaaggat cagtcagggc gggaagatcg tttgccacca
    41821 tcaccagacg gacgtgtgcc gggctcgcaa tggcaatctc cccctgcaac gcgccagggc
    41881 gacgcagcga ccactgggta atgcccagtt gctgtaactg ccagtctcgt cgggatgtca
    41941 tagcgaatcg ctcctgttgt caggggcgca aatatagcaa attcgtcgat accgcgccaa
    42001 catatggcta taatcgccgc cagtatcaat tgaggagcat tccatgtctg catttacccc
    42061 ggcaagtgaa gtcttgctgc gtcacagtga tgatttcgaa caaagccgta ttctgtttgc
    42121 cggagactta caggatgacc tgcccgcgcg tttagatacc gcggccagcc gtgctcatac
    42181 ccagcaattc caccattggc aggtgttaag ctgccagatg ggggataacg cccgttttag
    42241 tctggtcgcc acggcgaatg atgtcgcaga ttgcgatacg ctgatttact actggccgaa
    42301 gaacaaaccg gaagcccagt tccagttgat gaatttactt tctctgctgc cagtggggac
    42361 agatattttt gtcgttggcg agaaccgcag cggcgtgcgc agcgctgagc agatgctggc
    42421 agattatgcg ccgttgaata aagtcgacag cgctcgtcgc tgtggcctct attttggtcg
    42481 tctggaaaaa cagccagtat ttgatgcgga taaattctgg ggcgaataca gcgtcgatgg
    42541 cctgacggtc aaaacgctgc ctggcgtgtt tagccgcgac ggtctggatg tcggtagcca
    42601 gttgctgctc tcgacgttaa ctccgcacac gaaaggtaaa gtgctggatg tcggctgtgg
    42661 cgcgggtgta ctttcagttg cctttgcgcg ccattcgccg aaaattcgtc tcaccttgtg
    42721 cgatgtctct gccccggcgg tagaagccag ccgcgcaaca cttgcagcca acggtgttga
    42781 aggtgaagtc tttgccagca acgtcttttc cgaggtgaaa ggttgttttg atatgatcat
    42841 ctccaacccg ccgttccacg atgggatgca aaccagcctg gatgcggcgc aaacgctgat
    42901 tcgtggcgcg gtgcgtcatc ttaatagcgg cggcgagctg cgaattgtag cgaacgcctt
    42961 cctgccttac ccggacgtgc tggatgagac atttggcttc catgaagtta tcgcgcaaac
    43021 cgggcgcttc aaggtatatc gcgccattat gacccgccag gcgaagaaag gttgagtatc
    43081 tgatatctgt cgccgcatac cgtgtcggat gcggcgtgaa cgccttatcc gacctacaaa
    43141 acctgcacgt tagcccttcg taggccagat aagacgcgcc agcgtcgcat ctggcatctg
    43201 cccccgcatg cctaccaaac ctgcacgcta gtttcctgat ggacattttt ccagcaatta
    43261 cacatcaggc gataattaac tattgacgaa aagctgaaaa ccactagaat gcgcctccgt
    43321 ggtagcaatt ctttttaaga attgatggta tgcgaaggtg gcggaattgg tagacgcgct
    43381 agcttcaggt gttagtgtcc ttacggacgt gggggttcaa gtcccccccc tcgcaccaaa
    43441 aaccacgttg atattgctcg cactgggcga aggtggcgga attggtagac gcgctagctt
    43501 caggtgttag tgttcttacg gacgtggggg ttcaagtccc ccccctcgca ccaaacgagg
    43561 cgatatcaaa aaaagtaaga tgactgtgcg aaggtggcgg aattggtaga cgcgctagct
    43621 tcaggtgtta gtgtccttac ggacgtgggg gttcaagtcc cccccctcgc accaattatc
    43681 tttacttcct ttcttgtttc ttccctgttt tgtcatccct gattcaaatt cattatcatc
    43741 agcatgatgc cagcaataag cgccaggcac gatggtagca aaatgtaatg ccgcaactgt
    43801 ttgtgataca gcatcaccgg cgtcaatact aatcccatca gaatgacaat ccgccactga
    43861 tccggcgata aatccgcata catcagccct gcaatcagca atcccggcag caacactccc
    43921 cagccactgc ccagcgtacg ttgcaacatg gtttcatctc tttcattgat aatgaaaacc
    43981 aatatcatat gataattttt atcatttgca agccagagaa atcccttgct atcgagtaaa
    44041 cctatcgcta ggattagcaa tcattatcat ttagattact atcacgatta tggcctatcg
    44101 ttccgcaccg ctctatgaag atgtcatctg gcgaacgcat ctccagccgc aggatgctgg
    44161 gcttgcacaa gccgtacggg cgatgatcgc caaacatcgt gaacatttgc tggagtttat
    44221 ccgcctggat gaacccgctc cgcttaacgc catgacgctg gcgcaatggt catcaccgaa
    44281 tgcgctaagt tctctactgg cggtctattc cgatcatatc tatcgcaacc aaccaacgat
    44341 gatccgcgag aacaaaccgc tgatctcact atgggcacaa tggtatatcg gcctgatggt
    44401 gccaccatta atgctggcgc tactgacgca ggaaaaagca ttagatgtgt cgccggaaca
    44461 tttccatgct gagtttcacg aaaccggacg cgtcgcctgt ttctgggtcg atgtgtgtga
    44521 agataaaaac gcaacaccac attcgccgca gcagcgaatg gaaacgttaa tcagccaggc
    44581 gctggttccg gttgtgcaag cactagaagc gaccggagag atcaacggca aacttatctg
    44641 gagtaatacc ggttatttga tcaactggta tctcactgag atgaagcaac tgctcggcga
    44701 ggctactgtt gaatcgctgc gccatgccct cttttttgag aaaacgctca cgaacggtga
    44761 agataatcca ctctggcgta ccgtggtact gcgcgacggc ctgctggtgc gccgcacttg
    44821 ctgccagcgt tatcgcttac cggatgtgca gcaatgtggc tattgtacgc tgaaatgaaa
    44881 gcactgccgg ggaagtaaac ccggcatcat gcggattaat agggatgcaa cacattactt
    44941 gcttcgcaat gccgcataca taacagaata tctgcggcct ccaacaaccc tgcatcagaa
    45001 ctgacgccca gtttcgacat cacattaaat ttatgcgctc ggatcgtttt gatattacgt
    45061 ttaagctgct cggcaatttg tgtcattgag tagccacgcg acataaagcg caatatttct
    45121 ctctccgtcg ggcttaacgt ccggctttgg ttaatgtacc actgattgtt cagtcggtcg
    45181 gtcgcctgac gtacaccatt taatgacaaa aagagttcct gatgaaaaat ctccagcgtt
    45241 gacgctttac ttaatacacc gtccagcggt gatggcgaca atgaaccaat cagccgagct
    45301 tcaatatcat catccacaat aactaaacgc cgggtacgcg gaaacttaat cgccagctca
    45361 gtcaggcaag ataatccctc tctgcgctca cttctcatgg cagaaaaaga aaaaatgacc
    45421 gccgcaaacg agatccgcga catggcatcc tgaaatgact cctgactggt ataggtatgg
    45481 agcttataag ggttgcccgc aaactttctg gataaactct caataccgat actactcatg
    45541 acgcattttt ctatgagtgc gacatttctc ttcttaattc ggctgtgttc cattctgcgg
    45601 catctccata aacagaaggc aagttaagtt ctcttgcacc ctgcgcgcta tttatccatg
    45661 catacatttc agcgttgctg tgaattgcca gtcgacgcat cgcactgttt ttttgggcgc
    45721 tgatagtttt attacttttc ttaagcaatg aagctatctg gttgatgccc catcctttac
    45781 ctaatagccg cagtaccttg cgctctgaaa gagtgagaat gacggagcga tagctaaaat
    45841 catgagtttc agtcattgcc ggagaggtca gcatggcgct gatacgctct gcgtgcgtat
    45901 tgccggaacg tacggtcttg accagattct caatgggttc aacatcagac aataacgtcg
    45961 ccgtagggca catgagcagt tctacagcct gggaatacca ggagcgtgac accatgaaaa
    46021 cccagtgaat ttcccgatat tgtgagatta aagaataata atgttcacaa acagaacgtg
    46081 gatcttcact ttcaccggct aaatcagcaa tgactaat
//
LOCUS       DAEEUE010000025        45946 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.025, whole genome shotgun sequence.
ACCESSION   DAEEUE010000025 DAEEUE010000000
VERSION     DAEEUE010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45946
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.025"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..104)
                     /locus_tag="KLK84_001460"
     CDS             complement(<1..104)
                     /locus_tag="KLK84_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE2008837.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRNLCSH"
     gene            255..2408
                     /locus_tag="KLK84_001461"
     CDS             255..2408
                     /locus_tag="KLK84_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HBE2008838.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2461..4191
                     /locus_tag="KLK84_001462"
     CDS             2461..4191
                     /locus_tag="KLK84_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2008839.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4251..4742)
                     /gene="yajQ"
                     /locus_tag="KLK84_001463"
     CDS             complement(4251..4742)
                     /gene="yajQ"
                     /locus_tag="KLK84_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HBE2008840.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4910..5821
                     /gene="panE"
                     /locus_tag="KLK84_001464"
     CDS             4910..5821
                     /gene="panE"
                     /locus_tag="KLK84_001464"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBE2008841.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5784..6374
                     /gene="yajL"
                     /locus_tag="KLK84_001465"
     CDS             5784..6374
                     /gene="yajL"
                     /locus_tag="KLK84_001465"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HBE2008842.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6428..7876)
                     /gene="thiI"
                     /locus_tag="KLK84_001466"
     CDS             complement(6428..7876)
                     /gene="thiI"
                     /locus_tag="KLK84_001466"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBE2008843.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            8082..8324
                     /gene="xseB"
                     /locus_tag="KLK84_001467"
     CDS             8082..8324
                     /gene="xseB"
                     /locus_tag="KLK84_001467"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBE2008844.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8324..9223
                     /gene="ispA"
                     /locus_tag="KLK84_001468"
     CDS             8324..9223
                     /gene="ispA"
                     /locus_tag="KLK84_001468"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HBE2008845.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9248..11110
                     /gene="dxs"
                     /locus_tag="KLK84_001469"
     CDS             9248..11110
                     /gene="dxs"
                     /locus_tag="KLK84_001469"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBE2008846.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNCRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11165..12082
                     /gene="yajO"
                     /locus_tag="KLK84_001470"
     CDS             11165..12082
                     /gene="yajO"
                     /locus_tag="KLK84_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HBE2008847.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12193..12711)
                     /gene="pgpA"
                     /locus_tag="KLK84_001471"
     CDS             complement(12193..12711)
                     /gene="pgpA"
                     /locus_tag="KLK84_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBE2008848.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12689..13666)
                     /gene="thiL"
                     /locus_tag="KLK84_001472"
     CDS             complement(12689..13666)
                     /gene="thiL"
                     /locus_tag="KLK84_001472"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HBE2008849.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13744..14163)
                     /gene="nusB"
                     /locus_tag="KLK84_001473"
     CDS             complement(13744..14163)
                     /gene="nusB"
                     /locus_tag="KLK84_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBE2008850.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14183..14653)
                     /gene="ribE"
                     /locus_tag="KLK84_001474"
     CDS             complement(14183..14653)
                     /gene="ribE"
                     /locus_tag="KLK84_001474"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBE2008851.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14742..15845)
                     /gene="ribD"
                     /locus_tag="KLK84_001475"
     CDS             complement(14742..15845)
                     /gene="ribD"
                     /locus_tag="KLK84_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBE2008852.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15849..16298)
                     /gene="nrdR"
                     /locus_tag="KLK84_001476"
     CDS             complement(15849..16298)
                     /gene="nrdR"
                     /locus_tag="KLK84_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HBE2008853.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16449..16988
                     /locus_tag="KLK84_001477"
     CDS             16449..16988
                     /locus_tag="KLK84_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HBE2008854.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            17286..18170
                     /gene="tsx"
                     /locus_tag="KLK84_001478"
     CDS             17286..18170
                     /gene="tsx"
                     /locus_tag="KLK84_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HBE2008855.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18347..18694)
                     /locus_tag="KLK84_001479"
     CDS             complement(18347..18694)
                     /locus_tag="KLK84_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HBE2008856.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18823..19794)
                     /gene="secF"
                     /locus_tag="KLK84_001480"
     CDS             complement(18823..19794)
                     /gene="secF"
                     /locus_tag="KLK84_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HBE2008857.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19805..21652)
                     /gene="secD"
                     /locus_tag="KLK84_001481"
     CDS             complement(19805..21652)
                     /gene="secD"
                     /locus_tag="KLK84_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HBE2008858.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21680..22012)
                     /gene="yajC"
                     /locus_tag="KLK84_001482"
     CDS             complement(21680..22012)
                     /gene="yajC"
                     /locus_tag="KLK84_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBE2008859.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(22035..23162)
                     /gene="tgt"
                     /locus_tag="KLK84_001483"
     CDS             complement(22035..23162)
                     /gene="tgt"
                     /locus_tag="KLK84_001483"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBE2008860.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23217..24287)
                     /gene="queA"
                     /locus_tag="KLK84_001484"
     CDS             complement(23217..24287)
                     /gene="queA"
                     /locus_tag="KLK84_001484"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBE2008861.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24380..24953
                     /gene="acpH"
                     /locus_tag="KLK84_001485"
                     /pseudo
     CDS             24380..24953
                     /gene="acpH"
                     /locus_tag="KLK84_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24958..26775)
                     /gene="malZ"
                     /locus_tag="KLK84_001486"
     CDS             complement(24958..26775)
                     /gene="malZ"
                     /locus_tag="KLK84_001486"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HBE2008862.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGVLLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26931..28304)
                     /gene="proY"
                     /locus_tag="KLK84_001487"
     CDS             complement(26931..28304)
                     /gene="proY"
                     /locus_tag="KLK84_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HBE2008863.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28380..29699)
                     /gene="brnQ"
                     /locus_tag="KLK84_001488"
     CDS             complement(28380..29699)
                     /gene="brnQ"
                     /locus_tag="KLK84_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HBE2008864.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(30106..31401)
                     /gene="phoR"
                     /locus_tag="KLK84_001489"
     CDS             complement(30106..31401)
                     /gene="phoR"
                     /locus_tag="KLK84_001489"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HBE2008865.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31459..32148)
                     /gene="phoB"
                     /locus_tag="KLK84_001490"
                     /pseudo
     CDS             complement(31459..32148)
                     /gene="phoB"
                     /locus_tag="KLK84_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
     gene            32338..33540
                     /gene="sbcD"
                     /locus_tag="KLK84_001491"
     CDS             32338..33540
                     /gene="sbcD"
                     /locus_tag="KLK84_001491"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HBE2008866.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQMDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33537..36680
                     /gene="sbcC"
                     /locus_tag="KLK84_001492"
     CDS             33537..36680
                     /gene="sbcC"
                     /locus_tag="KLK84_001492"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HBE2008867.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLIVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHKRQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36806..37987
                     /gene="araJ"
                     /locus_tag="KLK84_001493"
     CDS             36806..37987
                     /gene="araJ"
                     /locus_tag="KLK84_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HBE2008868.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGGAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(38130..39038)
                     /gene="mak"
                     /locus_tag="KLK84_001494"
     CDS             complement(38130..39038)
                     /gene="mak"
                     /locus_tag="KLK84_001494"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HBE2008869.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVTIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            39163..40074
                     /gene="rdgC"
                     /locus_tag="KLK84_001495"
     CDS             39163..40074
                     /gene="rdgC"
                     /locus_tag="KLK84_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HBE2008870.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(40152..40607)
                     /locus_tag="KLK84_001496"
     CDS             complement(40152..40607)
                     /locus_tag="KLK84_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2008871.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40604..40939)
                     /locus_tag="KLK84_001497"
                     /pseudo
     CDS             complement(40604..40939)
                     /locus_tag="KLK84_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(41094..41378)
                     /gene="ppnP"
                     /locus_tag="KLK84_001498"
     CDS             complement(41094..41378)
                     /gene="ppnP"
                     /locus_tag="KLK84_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBE2008872.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41450..42127)
                     /locus_tag="KLK84_001499"
     CDS             complement(41450..42127)
                     /locus_tag="KLK84_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HBE2008873.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42388..42576)
                     /gene="yaiA"
                     /locus_tag="KLK84_001500"
     CDS             complement(42388..42576)
                     /gene="yaiA"
                     /locus_tag="KLK84_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HBE2008874.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDK"
     gene            complement(42626..43150)
                     /gene="aroL"
                     /locus_tag="KLK84_001501"
     CDS             complement(42626..43150)
                     /gene="aroL"
                     /locus_tag="KLK84_001501"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HBE2008875.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43333..43791)
                     /locus_tag="KLK84_001502"
     CDS             complement(43333..43791)
                     /locus_tag="KLK84_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBE2008876.1"
                     /translation="MTIWVDADACPNVIKEILYLAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43911..44720
                     /gene="proC"
                     /locus_tag="KLK84_001503"
     CDS             43911..44720
                     /gene="proC"
                     /locus_tag="KLK84_001503"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBE2008877.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44737..45852)
                     /gene="adrA"
                     /locus_tag="KLK84_001504"
                     /gene_synonym="dgcC"
     CDS             complement(44737..45852)
                     /gene="adrA"
                     /locus_tag="KLK84_001504"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HBE2008878.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11955 a        11979 c        11237 g        10775 t
ORIGIN      
        1 caatgagagc agagattgcg ctgatgtccg gcagtgcttt tgccgttacg caccacgcct
       61 tcagtagcgg agcaggaagg acatctgatg gaaatggaag ccacgcaagc accttaaaat
      121 caccatcata cactaaatca ataagttggc agcattacct tcgcttaatt ccatataact
      181 ggacgattac attagaagta aactatactt tttatgaaat atcagggata aattattcac
      241 atggaaaata catcatgtac aaatcattat ttactattat cattctactg tatagcgcca
      301 gttcatatgc aaaaagcgaa tcccctcccg caagcactat aactttcatt gcacaaatcg
      361 atcatgttga gctaaataaa acagcattat ctcatgatat gaaactcgtt cttgccgatc
      421 gtttttattt caaaaccagt aatccttgcg aaacaaattc aatttctgct cgaccagaat
      481 caatggatat tactgacaaa gcctatgccg acaaattaaa aaaattagaa actccttttc
      541 ttgatgatcg ctatttcttt atgaccatca atcagtgtga cggtaatggc actcccatgc
      601 tgacagacat aaaagtatgt acaacagaat tatgtggtgc tgaatataca aagaaaatgt
      661 ctactgtatg gctcgatgaa actttaaaac caactgctaa acgtctggca atttatttta
      721 ttcaatctcc tttaccctat gacgaggaga aaaaacaatg gaaagtcacc ggatggtata
      781 tctcatcaac tgataatcag tcttcaacta aacagattgc atttgaagga tatacagaca
      841 cagataacct ctccgattct aaatttattt cttcatttaa atcctggttt gaaagtggaa
      901 agataaaaaa cattgctaac tataatccaa aaggagaaag agatggaaaa tacgaaagtt
      961 attacaatag caatggtaac gtctatgaat ctctaagttt ttctaatggt cttatcaacg
     1021 gtgagtttat tacatattat gaaaatgggg ccattgaaag taaacgtcat tttaataacg
     1081 acaaaattaa agatggtgaa tgccctcatt tttatgaaga tgggaaaatc aaacagcaac
     1141 atagctatct gaatagtaaa ctcgatggtc ctgcatatga atattatcct gatggcaaac
     1201 tcatgcagga aaatttctat cagcaaagtg tgttgattgg aaacgatacc agttactacc
     1261 aatccggaaa aataagctct atacacaata gaaattcccg tggtcagtat gatggagcta
     1321 acgaacgtta tagtgaagag ggtaagttac tgtctaaatc cgtctataaa gacggaaaac
     1381 aaatttctgt tcaaacctgg tatgaaaatg ggcagaaaga agaggagaaa cattttgatg
     1441 agcaaggcca attaaatggt ctggttaagc aatggtataa aaatggtaat cttgcaaaat
     1501 cacaaaacta taaacatgac atactcgatg gtaacagtga agagtggtac gaaaatggca
     1561 tccctgaaag tttgtaccct tataagaatg gaaaaaccga cggcattgcc aaatcatgga
     1621 ataagtatgg taaattaacc tattcaatcg aatacaaaaa tggcgtagag aatggggcat
     1681 atcgtaactg gagcaaaaac acaggtaaac taaccaaaga aaccctgtac gtcaatggta
     1741 tcagacaggg agtggaaaaa gagtttaacg atcgcacagg taaactatta acctccacgc
     1801 aatatgttaa taataaaaga catggtacgg aggaaactta tgatcaaaac ggaataaaat
     1861 atattacatg ctatcaaaat gatcagaaat tatcgtcttt agacaatcca acacaaataa
     1921 aagataatgc aacaacaggc gattccagtg ctcaatttgc attagggaaa tacgaattca
     1981 tctgtgcaaa tatagatgaa gggattaaat ggttaaccaa atctgcagag caaaaaaaca
     2041 ctgatgcgat atacttttta gctacagcct acaaaggcaa tggtatacct gcaaacaatg
     2101 aaaaatacct agcatatctg caacaagctg caacactggg cagtagtaat gcccaggcgg
     2161 aaattggcta tttatatctc atcggaaaag aactaccaca gaatcttcct gatgctggtg
     2221 tctggttcaa aaaagcagct gcacaaggta attttgccgc acatttctat ctgggaagga
     2281 tgtatcagaa tggtgacggc gttgagagaa atatggaaaa agccaggttt catctatcta
     2341 atgcggcaga aggtggaata gaacctgcgc ttaaagcatt aaatgaactt gaacatcaaa
     2401 caaaataaag cgctcaggga ccacgtaagg ttcatcaaac agaaaaggat atagtttgcg
     2461 atgaaagcga aatctacttt gttattaatg attttatctg ttttttattc tcatcaggtt
     2521 ttatccagcg aacccactca gccggatgtt tattctgtcg tcgagaaaaa actaaactct
     2581 gctttacctc tggaagaaaa taccaccttc agatcgcagg ctgaatggtt taatttgcaa
     2641 tatgaattat tagtatctgg ctaccctgaa cgtgcttaca atttactgag caaacttgag
     2701 acagaatcga aaaacgctaa atattatctg gatttaactg cctccagagt cgctgaagaa
     2761 gaagcaccag agacagcctt aactttttta tcaaaaattg ggttaaataa accggctact
     2821 cttacatcct ttgagagcta tatcaatagc tggataaaaa gcaaacaacc cgaaaaggct
     2881 atgcagcttc tcagcctcga tgagaatgcc ctgaattatt atttaccgac tgtgttagtc
     2941 gcatatcaga tcgttcctga taaggcaata agtatttata attctgtata tggagataat
     3001 gttgttatac cctatcagca agtcaccatg ttacttacag ttgcggaggg ttatcaaaca
     3061 aaaggcgata taaaaaacgc cattcattat gccgataagg cgctgaatat gtttgataaa
     3121 gctattgctg tagatagcag tcgtgaatcc cattatagtg atgagtattt gaaactcatg
     3181 aatatctacg ccgccaatgg caacaaagaa aaagcggtgg ttttatctca acgtctacac
     3241 aaagccatta ccgaatctca atcgaattac ttaagtactc tggaaggaat tttattgttt
     3301 tatagaaata atgatatgca gcaagactat caaaacagcc tttcaaatta catagtattt
     3361 atcgataaaa tttttgcctt ttccccatca acaagaagtg aattaagcct cattaattta
     3421 ttaaattcat taaatgaggt ggaattgatg agaaaacgtc tggcgtttct gatgacagca
     3481 ccagaatacg cttgttatga tagtcagtat tgttatgaag acaaagtcag cgcattaaaa
     3541 atcctgtatc gtcatcatga caatgagcta atggacaaat atttcacgac tcttcttaaa
     3601 gagatcagta atcaaccatt agaagactgg gatataataa taagtaatac tggtgaaaaa
     3661 tttagtgaag tgggtctaac caaatatgct caacatctcg catctttcgc tgaagaattt
     3721 tatataaaag agcaaaaatc acattctgaa agtgaaatga gaggactatt ttcatcactt
     3781 gccgaattat atagttttgg taatgatact gccagcgcaa accgagtgtt ccatcagcat
     3841 gtccctgcct tcaatacaga tcatatgatt caatatttta taaatgccaa agaatggaac
     3901 aatgccaggg aattattgat aaaagaggaa atgttgggta tgcataatct gatattgttg
     3961 gagaatattt gtatgcaaaa aaatgcagaa tgcatggcac acattacctt tacgcttaat
     4021 aaattaacaa cacaaccagc aataacaaaa atagatagcg tgggtaatga gcaactttat
     4081 cagattggca aaatctacca taagttggaa attaaacctg agccagagca acaggtttta
     4141 attcagtcgc tttatgaccg ggcatcagga agcgcctctg caactcaata aatgcatggt
     4201 caaaagggcc accacggtgg ccctgacaat cagtagctat atccagaaag ttaatcgcgg
     4261 aagtttttga actggaacgg ctgaccgaga tcgccaccgc gcaccatcgc catgacagcc
     4321 tgcaaatcat cacgagattt gccagtcacg cggatctcat cgccctgaat ttgcgcctgc
     4381 actttcagtt tgctgtcttt gatcatcttg acgatttttt tctgagtggc gctctcaatg
     4441 ccctgtttca gtttcgcttc cacaaaccag gttttaccgc tatgaacgat attttccggt
     4501 acatccagtg aactgccttc aatgccgcgc ttcagcagct tggcacgcag aatatccagc
     4561 aactgattga cctggaagtc ggactcgctc aacactttga tggttttgct ggcgtcgttc
     4621 agctcaaatg aggcttcaac gttacggaag tcaaaacggg actccacttc gcgactcgcg
     4681 ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag agacaatatc gaaagatggc
     4741 atcttttcct ctcccttcat ttttgatgcg aagcataata cctgcaaagt taaataacca
     4801 acagtcctgc accaggacgt atcccttaat tgtgcatagt cgcaacagca tgcacaatta
     4861 agggatagcc ttattgaaca cccggagtgg ttgcgggtga ggaggaacaa tgaaaattac
     4921 cgtattggga tgcggtgcct tagggcaatt atggcttaca gcactttgta aacagggtca
     4981 tgaagttcag ggctggctgc gtgtaccgca accttattgt agtgtgaatc tggttgagac
     5041 agatggttcg atatttaacg aatcgctgac cgccaacgat cccgattttc tcgccaccag
     5101 cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc gatgccgtca aaagcctcgc
     5161 atccacactg cctgtaacta cgccaatact gttaattcac aacggcatgg gcaccatcga
     5221 agaattgcaa aacattcagc agccattact gatgggcacc accacccatg ccgcccgccg
     5281 cgacggcaat gtcattattc atgtggcaaa cggaatcacg catatcggtc cggcacggca
     5341 acaggatggc gattacagtt atctggcgga tattttgcaa accgtgttgc ctgacgttgc
     5401 ctggcataac aatattcgcg ccgagctgtg gcgcaagctg gccgtcaact gcgtgattaa
     5461 tccactgact gccatctgga attgcccgaa cggtgaatta cgtcatcatc cgcaagaaat
     5521 tatgcaaata tgcgaagaag tcgcggcagt gatcgaacgc gaagggcatc atacttcagc
     5581 ggaagatttg cgtgattacg tgatgcaggt gattgatgcc acagcagaaa atatctcgtc
     5641 gatgttgcag gatatccgcg cgctgcgcca caccgaaatc gactatatca atggttttct
     5701 cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa aacacccgcc tgtttgaaat
     5761 ggtaaaaaga aaggagagtg aatatgagcg catcggcact ggtttgcctc gcccctggta
     5821 gtgaagagac tgaagccgtc accacgatca acctcttggt tcgcggcggt atcaaagtca
     5881 ccactgccag cgtcgacagc gatggcaacc tggcaattac ctgctcgcgc ggcgtgaagc
     5941 tgctggcgga tgcgccgctg gtcgaagtgg ctgatggcga atatgacgtg atcgtgctgc
     6001 ctggtggcat taaaggcacg gagtgttttc gcgatagcac tctgctggtt gaaaccgtta
     6061 aacagttcca ccgttccggg cgtatcgtcg cggctatttg cgccgcgcca gccaccgtgc
     6121 tggtgccgca cgatatcttt ccgattggca atatgaccgg cttcccgacg ctgaaagaca
     6181 aaattcccgc tgaacaatgg caggacaagc gcgtcgtctg ggatgcacgg gtaaaattgc
     6241 tgaccagcca ggggccgggt acagctatcg actttggtct gaaaattatc gacctgctgg
     6301 ttgggcgcga gaaagcccat gaagtggcat cacaactggt gatggcggca gggatttata
     6361 attattacga gtagtgtcgg atgcggcaaa cgtcgcatct gaccagatgc gacgtaaaaa
     6421 ctacaaatta cgggcgatat accttcacat tgttaaagcc ttgctcgcgc agatagagcg
     6481 cctgcagacg gctcatcacc ccgcgctcac accacagcag ccaggttttg ttctggtcga
     6541 gatcgccaaa tttggtgctc agtttataga acggcagaga aaccacatca atcccttcga
     6601 ctttcagtgg cttatcttcc tgttcatcga cagaacggat atcgagaatc acgtcgttcg
     6661 ggccgaagcc gttgacggtt tccacttcca ccacttcctg ctcggtctgc tgggcgattt
     6721 cgcggatatc aacgttattc gcttcctcaa ccactttatc gagaatactg aagtcgaact
     6781 tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac cgtcgggctt ttggagatca
     6841 cgccacaata ttccggcatc gtgcgagcaa agtcttcggt gccaatctgg cgagccaggt
     6901 tgatgatgtg ctctttgtcg taagaaatca gcggacgcag gatcagcgtg tcagagacgt
     6961 tatcaatcag gcgcaggttg gtcagcgtct gactggacac ctggccgagc gcttcgccgg
     7021 tgaccagcgc ctgtacgccg taacgttcag ccactttaga tgcggcacgc accatcatac
     7081 gtttgaggat aacgcccatc tgaccgtcgt cgattttctc gagaatttcc ccgacaaccg
     7141 gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct gccaaagcgg ttccacagat
     7201 aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc gccgaggtta aagaagcagt
     7261 aatgcacgcg gcagccgcga cgcatcaaca tataactgga aacaccggag tcgaaaccac
     7321 cggaaatgag cgacagcaca tcttcctggg tgccaatcgg gaaaccacca ataccttcgt
     7381 agcgaccttt aatcagcagg aggcgatcgt cttccacttc cagatgaaca gttacatccg
     7441 ggttagtcag cttcacgcgc gcggattcaa tatgctgatt taaaccgccg ccgacgtaac
     7501 gttccacatc aatcgagcta aaatcatgtt tgccacggcg cttcacgcgt acgcagaagg
     7561 ttttgccttc cagctgatcg cgatactgaa ccaacgcttt ctcaaaaata tcgtgcatgt
     7621 cggtaaacgg cacgtcttcg acttcgagaa tatggtggat acccggaata cgggtcaggg
     7681 cgtcgcgaat agtcagacgc tggttttcat ctttcgcgcg aacttcgatg ttatcccagt
     7741 ggcggacgac agcgagcgtc tcatcatagt gctttaaaac gttacgaatg ttcccggtaa
     7801 ggatttttat aaagcgcaag cgcacagatt ggcttttgat ggtgatttcc gggaacaatt
     7861 taatgataaa cttcatggcg gcaatggttc gttggcaagc cttaagcgac ttgtataggg
     7921 aaaaatacag cagcccacac ctgcggctgc atccaggcgc ggaagtatac cactaacatc
     7981 gctttgctgc gcacatcacc ttaccattgc gcgttatttg ctatttgccc tgagtccgtt
     8041 accatgacgg ggcgaaaaat attgagagtc agacattcat tatgccgaag aaaaatgagg
     8101 cgcccgccag ctttgaaaag gcgctgagcg agctggaaca gattgtaacc cgtctggaaa
     8161 gtggcgacct gccgctggaa gaggcgctga acgagttcga acgcggcgtg cagctggcac
     8221 gtcaggggca ggccaaatta caacaagccg aacagcgcgt acaaattctg ctgtctgaca
     8281 atgaagacgc ctctctaacc ccttttacac cggacaatga gtaatggact ttccgcagca
     8341 actcgaagcc tgcgttaagc aggccaacca ggcgctgagc cgttttatcg ccccactgcc
     8401 ctttcagaac actcccgtgg tcgaaaccat gcagtatggc gcattattag gtggtaagcg
     8461 cctgcgacct ttcctggttt atgccaccgg tcatatgttt ggcgttagca caaacacgct
     8521 ggacgcaccc gctgctgccg tagagtgtat ccacgcttac tcattaattc atgatgattt
     8581 accggcgatg gatgatgacg atctgcgtcg cggtttgccg acctgccatg tgaagtttgg
     8641 cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg ctggcgttct cgattctaag
     8701 cgatgccaat atgccggaag tgtcggatcg cgacagaatt tcgatgattt ctgaactggc
     8761 gagcgccagc ggtattgccg gaatgtgcgg tggtcaggca ttagatttag acgcggaagg
     8821 caaacacgta cctctggacg cgcttgagcg tattcatcgt cataaaaccg gcgcattgat
     8881 tcgcgccgcc gttcgccttg gtgcattaag cgccggagat aaagggcgtc gtgctctgcc
     8941 agtactcgac aagtacgcag agagcatcgg ccttgccttc caggttcagg atgacatcct
     9001 ggatgtggta ggagatactg caacgttggg aaaacgccag ggtgccgacc agcaacttgg
     9061 taaaagtacc taccctgcac ttctgggtct tgagcaagcc cggaagaaag cccgggatct
     9121 gatcgacgat gcccgtcagg cgctgaaaca actggctgaa cagtcactcg atacctcggc
     9181 actggaagcg ctagcggact acatcatcca gcgtaataaa taaacaataa gtattaatag
     9241 gcccctgatg agttttgata ttgccaaata cccgaccctg gcactggtcg actccaccca
     9301 ggagttacga ctgttgccga aagagagttt accgaaactc tgcgacgaac tgcgccgcta
     9361 tttactcgac agcgtgagcc gttccagcgg gcacttcgcc tccgggctgg gcacggtcga
     9421 actgaccgtg gcgctgcact atgtctacaa caccccattt gaccaattga tttgggatgt
     9481 gggacatcag gcttatccgc ataaaatttt gaccggacgc cgcgacaaaa tcggcaccat
     9541 ccgtcagaaa ggcggtctgc acccgttccc gtggcgcggc gaaagcgaat atgacgtatt
     9601 aagcgtcggg cattcatcaa cctccatcag tgccggaatt ggtattgcgg ttgctgccga
     9661 gaaagaaggc aaaaattgcc gcaccgtctg tgtgattggc gacggcgcga ttaccgctgg
     9721 catggcgttt gaagcgatga atcacgcggg cgatattcgc cctgatatgc tggtggtcct
     9781 caacgacaat gaaatgtcga tttccgaaaa tgttggcgcg cttaacaacc atctggctca
     9841 gctgctttcc ggtaagcttt actcttcact gcgcgaaggc gggaaaaaag ttttctctgg
     9901 cgtgccgccc attaaagagc tgctcaaacg taccgaagaa catattaaag gcatggtggt
     9961 gcctggcacg ttgtttgaag agctgggatt taactacatc ggcccggtgg acggtcacga
    10021 tgtgctgggg cttatcacca cgctaaagaa catgcgcgac ctgaaaggcc cgcagttcct
    10081 gcatatcatg accaaaaaag gtcgtggtta tgaaccggca gaaaaagacc cgatcacttt
    10141 ccacgccgtg cctaaatttg atccctccag cggttgtttg ccgaaaagta gcggcggttt
    10201 gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa acggcagcga aagacaacaa
    10261 gctgatggcg attactccgg cgatgcgtga aggttccggc atggtcgagt tttcacgtaa
    10321 attcccggat cgttacttcg acgtggctat tgccgagcaa cacgcagtga cctttgccgc
    10381 cggtctggcg attggtggat acaaacccat tgtcgcgatc tactccacct tcctgcaacg
    10441 cgcctatgat caggtgctgc atgacgtggc aattcaaaag ctaccggtcc tgttcgccat
    10501 tgaccgcgcg ggcattgttg gtgctgacgg tcaaactcac cagggcgctt ttgatctctc
    10561 ttacctgcgc tgcataccag aaatggtcat tatgaccccg agcgatgaaa acgaatgtcg
    10621 ccagatgctc tataccggct atcactataa cgatggcccg tccgcggtgc gctacccgcg
    10681 cggcaacgcg gttggcgtgg aactgacgcc gctggaaaaa ctaccaattg gcaaaggcat
    10741 tgtgaagcgt cgtggcgaga aactggtgat ccttaacttt ggtacgctga tgccagaaac
    10801 ggccaaagtc gccgaatcgc tgaacgctac gctggtcgat atgcgttttg tgaaaccact
    10861 tgatgaagcg ttaattctgg aaatggccgc cagccatgaa gcgctggtca ccgtagaaga
    10921 aaacgccatt atgggcggcg caggtagcgg agtgaacgaa gtgctgatgg cccatcgtaa
    10981 accagtaccc gtgctgaaca ttggcctgcc ggacttcttt attccgcaag gaactcagga
    11041 agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg gaagccaaaa tcaaggcctg
    11101 gctggcataa tccctactcc actcctgcta tgcttaagaa attattcatg gcaggagtgg
    11161 cagcatgcaa tacaacccct taggaaaaac cgaccttcgc gtttcccgac tttgcctcgg
    11221 ctgtatgacc tttggcgagc cagatcgcgg taatcacgca tggacactgc cggaagagag
    11281 cagccgtccc ataattaaac gcgcgctgga aggtggcata aatttctttg ataccgccaa
    11341 tagctattcc gatggtagca gcgaagagat cgttggtcgc gcactgcggg atttcgcccg
    11401 tcgtgaagac gtagtcgttg ccaccaaagt gttccatcgc gttggtgatt taccggaagg
    11461 attatcccgt gcacaaattt tgcgctctat caacgacagc ctgcgacgtc tcggcatgga
    11521 ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac acgccgatcg aagagacgct
    11581 ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt tatatcggcg cgtcatccat
    11641 gcacgcttcg cagtttgctc aggcgctgga actacaaaaa cagcactgct gggcgcagtt
    11701 tgtcagtatg caggatcact ataatctgat ttatcgcgaa gaagagcgcg agatgctgcc
    11761 tctgtgttat caggagggcg tggcggtgat tccgtggagc ccgctggcgc gggggcgact
    11821 gacgcgtcca tggggagaaa ctaccgcacg actggtgtct gatgaggtgg ggaaaaatct
    11881 ctataaagaa agcgatgaaa atgacgcgca gatcgcagag cggttaacgg gcgtcagtga
    11941 agaactcggt gcaacacgag cacaagttgc gctggcctgg ttgttaagta aaccgggcat
    12001 tgccgcaccg attatcggaa cttcgcggga agaacagctt gatgagctgc tgaacgcggt
    12061 ggatatcact ttgaagccat aacagattgc cgagctggaa acgccgtata aaccgcatgc
    12121 ggtcgtagga tttaaataag agtccaggcc tgatgagacg tgacaagcgt cacatcaggc
    12181 atcggtgcac aactacgaca gaatacccag cggccagtga tgaccgataa agtacaggat
    12241 gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg atccccatgc cgccatgtac
    12301 attgcgatca aaccagcgga tcggccacgg cttccacata tccagaatac ggaaaatcac
    12361 aaacccggcg gcaacccact gccagtcatt ggtcggcagc gccatgagcg tgatccacat
    12421 accaataaat tcgtcccaga caatgctgcc gtgatcgtgc acgcccatat ctttcgccgt
    12481 ttgatgacaa agatagacgc cgatacagat ccccaacatc accaccagcg agtagagctg
    12541 ccagggtaaa aaggtcatca gataccagaa agggatcgcc gccagcgatc ccatcgtgcc
    12601 aggaacgatt gggcttaatc cacttccgaa tccgacagca agcagatgcc acggattact
    12661 catcttcagg cgacttttcg cgacatcttt atggcgtgac aaaatggtca tatcctttcc
    12721 agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag cccttcgata tcggcggtca
    12781 tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag agcaacatcc agcgcgccac
    12841 ggttcagttc cggcaccgtg aaacacaact cgtaatcttc accgccagag agcgcccagc
    12901 gcagcgcctg ttccggttca acatggcgag aaagcgcatc agaaaacggc agcaatgcca
    12961 ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg cccgagatcg gaaatcagac
    13021 cgtcagagag atcgatagct gaatttgcca gatcgcgcag tgcctgcccc tgtaaaatac
    13081 gcggcgatgg acggagatga cgtttgatca agtagtcagc atctttagca tcggcaacct
    13141 gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc gcccggtgta ccggtcacat
    13201 agatccagtc acccggtttc gccccagagc gcgttaaggc tcgtcccatc ggaacaaagc
    13261 cgtggatacc caacgtcatt gataatggcc cacgcgtggt atcgccgcca atgagttgca
    13321 tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa ggactcaagc cacgcttcgt
    13381 ctacgtccgg taaggttaat gccagcgtca gccaggccgg atcggccccc atcgctgcca
    13441 gatcgcttag gttcaccgcc agtgctttat aagccagatc agcaggatcg atatcaggga
    13501 ggaaatggtt tcccgccacc agcgtatcag tgctgatcgc cagggtctgt ttctcgggga
    13561 tattgagaag agcgcaatcg tcgccgatgc ccagttcgac atcaagacga gaacttctta
    13621 cacggtcaaa ataacgggca atcagggaga actcgccaca tgccatacgt tatgcctcag
    13681 caggtaaaaa agaaaaggcc ggcgatcatg gaacagtctt ccgtgaatct accggcctgg
    13741 atattacttt ttgttagggc gaatcacagg tgctgcttta tcgagtacgc cgttgacgaa
    13801 cttatggctg tcttctgcgc cgaacgattt cgccagttcg atcgcttcgt taatggccac
    13861 tttgtatggc acatcactac gtttagacag ttcgtacagc gcaatgcgca gtactgcttt
    13921 ttctacctgg cccagttctt ccagcaggcg ggacaggtag ggcttcatca gtccgtcgag
    13981 gtatgcggtg ttagtcgcca ccccggccag cagctcacgg aagtacagga cgtcaacgtc
    14041 ttttacatcc tgttcagcca ggaactggta ttcaacatca gcgatgtcgt tctgggacaa
    14101 ctgccaggag tagagcgcct ggacggcaca ctcacgagcg cggcgacgag cagcaggttt
    14161 cacggatttc cccttactaa tttcaggcct tgatggcttt caatacatta atcatttcaa
    14221 gcgcggtcag tgcagcttct gcacctttgt tgccagcttt ggtgccagca cgttcgatcg
    14281 cttgttcaat gctttcagtg gtcagaaccc caaaagcaac cggaatttcg ctgtcctggg
    14341 caacatgcgc caggccgttg cttgcaccac cagcgacata ttcaaagtgg gcagtgccac
    14401 cacgaataac cgtacccagc gcaatcaccg cgtcgtattt accggtttta gccagtgcac
    14461 ccgccgccag cggcagctca taggcaccag gcacccaaac aacggtaatg ttttcatctt
    14521 ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc cagcaggctg tcattgataa
    14581 agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc cggggtagca acgttagctt
    14641 caataatgtt cattttcttc cttcgggttc gagtatggcc ccgcaggggg gcggatttta
    14701 gcataatatt tcgtgcgctg cttccctttc gagccgggag atcatgcacc cactaaatgc
    14761 aggcaaacat ccgggcctac atgacgtatc tctttgaatt taaattgggg ggcgtcggct
    14821 aatttctcaa gccctggcag cgagcataat ccacgggcgt cgctgcctaa tagtttaggt
    14881 gcgatataga caatcagctc atcgactaaa cccgcctgta gcaatacgcc agcgagcgtt
    14941 ggccccgctt ccacccagat gctgttaatc tgctgtttac ccagttgcat catcagtaca
    15001 accagatcca gatgaccttt atgctctgga atcagcaagg tacgcaccgt ttccggccac
    15061 tcacgagaat cttcctgcgt acgcgcgaac caggtttcgc cgggctgctg cacaatgcga
    15121 tgttccggcg tcacgcgatt ttggctatca atcacaatac gtactggctg gcggagattt
    15181 tgttgcgggt agagcgcctg agtttgttca tccagttcag accaacgcac tgttaaggct
    15241 ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg catgactttg tgcgcgctgt
    15301 cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact ggctttcgcc gctcgccatc
    15361 gccgtgcggc catcaagcga tgcgccaagt tttaactgaa tataaggaaa gccggtgcgc
    15421 atccgcttga gaaagccttt attcaattgc tcggcttcac tcatcatcag cccgtggctg
    15481 acgtcaatgc cagcctgttg cagacggtaa agtccacgcc cagcgacctg cgggttagga
    15541 tcttgcatcg ctgcgaccac gcgcgctacg ccagcggcga tgagtgcgtc acagcacggt
    15601 ggcgtacggc catgatggct acagggttcg agtgtcacat acgccgttgc gcctttggct
    15661 ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg gttcacccgc tcgttggtgg
    15721 taaccttcac cgacaatttc gccatctttg acaatgacgc acccgacatt cgggttgggg
    15781 tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc gcgccatgta atactcgtcc
    15841 tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa attctttgat atcttcgaaa
    15901 ctgcggtaga cagaggcaaa acggatatag gcgactttat cgagcttttt caattgctcc
    15961 atcaccagat tgccaatcat cttgctcggt acttcgcgct caccggtggc gcgcagctgc
    16021 gatttaatat gattgattgc catttcaacg taatcggaac tcaccggacg tttttccagc
    16081 gcccgcagca ttccgctacg caatttctct tcattaaacg gttcacgcac gtcgttgctt
    16141 tttacaacac gcggcataac cagctccgcc acttcaaagg tggtgaaacg ttcattacac
    16201 accagacact gccgacggcg gcgtacggat gaaccctcgc ccacgagacg agagtcaatt
    16261 accttagtgt ccacggcgaa acagaatggg caatgcatac ggcgtcctga ccaggtggtt
    16321 aacggaaatc tattttaccc tgaactgacg atacaacaaa ggcgcaacgc ttttgagata
    16381 aaggatcgat gctttcgctg cttttaccct ctaccattag gctaattgcg acgagaaagg
    16441 gacgacctat gaatactaac gtttttcgac tgctcctgct gggaagcctg ttcagcctta
    16501 gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca cagcgtcagc acgctgaacc
    16561 aggagatgac gcaactcaat caagaaacag tcaaaatcac tcagcaaaac aggctgaatg
    16621 caaaatccag cagtggggtt taccttctgc ctggagcgaa aacaccggca agactggaaa
    16681 gccagatcgg tactttacgt atgtcgctgg tgaatattac gcctgataca gatggcacca
    16741 cccttacact acgtattcag ggtgagtcga atgatccctt gcccgcattc agcggaactg
    16801 ttgaatatgg gcaaattcag ggaacaatag acaattttca ggaaatcaat gtgcagaatc
    16861 aattgattaa tgcccctgcc agcgtcctcg cccccagcga tgttgatatt ccgttacaat
    16921 taaaggggat ttctgtggat cagttaggct ttgtacgtat ccacgacatt caacctgtta
    16981 tgcagtaaac gtatttcggg acgattttgt gcgtcccgca acatcttccc cgtcattttg
    17041 ttactctgct tacatcacct ggattgatag taaaagtttg caacaagggc gaaagtcagt
    17101 acaatccccg cccgaatgtg tgtaaacgtg aacgcaatcg attacgtaaa tgatagaact
    17161 gtgaaacgaa acatattttt gtgagcaatg atttttataa taggctcctc tgtatacgaa
    17221 atatttagaa acgcaatttg cgcctttttc actcccgcaa gggattttca aacagtggca
    17281 tacatatgaa aaaaacatta ctggcagccg gtgcggtact ggcgctctct tcgtctttta
    17341 ctgtcaacgc agctgaaaac gacaaaccgc agtatctttc cgactggtgg caccagagcg
    17401 ttaacgttgt cggaagctat cacacccgtt tcggaccgca gatccgcaac gatacctacc
    17461 ttgagtacga agcattcgct aaaaaagact ggttcgactt ctatggttat gcggatgcgc
    17521 cggtattctt cggcggtaac tccgatgcta aaggtatctg gaaccacggt tctccgctgt
    17581 ttatggaaat cgaaccacgt ttctccatcg acaagctgac caatactgac cttagcttcg
    17641 gtccgttcaa agagtggtac ttcgcgaaca actacattta cgacatgggt cgtaataaag
    17701 atggtcgcca gagcacctgg tacatgggtc tgggtaccga tatcgacact ggcctgccga
    17761 tgagcctgtc catgaacgtc tatgcgaaat accagtggca gaactatggc gcagcgaacg
    17821 aaaacgagtg ggacggttac cgtttcaaaa ttaaatactt tgtgccgatt accgatctgt
    17881 ggggcggtca gctgagctac atcggcttca ccaacttcga ctggggttcc gatttagggg
    17941 atgacagcgg taacgcaatc aacggtatta agacccgtac taataactct atcgcttcca
    18001 gccatattct ggctctgaac tacgatcact ggcactactc tgtcgtagct cgttactggc
    18061 acgacggtgg tcagtggaac gacgatgcag aactgaactt cggcaacggc aacttcaacg
    18121 ttcgctctac cggctggggt ggttacctgg tagtaggtta caacttctga ttatgaaaat
    18181 gccgggattt attcccggca tttctgattg ttaacaacgt gtgctttgtt catgccggat
    18241 gcggcgtaaa cgctttgtcc ggcccacaaa agcacgtaaa ttcaatatat tgctgaaata
    18301 acgtaggcct gataagcgta gcgcatcagg caattttacg ttttaatcac ttcttcttgt
    18361 tcatcatcgc tttcaggtca gcgaatgggt tgtacttcgc ttcaccgaca tctttctgcg
    18421 catcttcccc tgcgataacg gtcgtaccat actgatccgc ttcggtatat ttcgaatgct
    18481 catgatcgtg gcaatagaga cacaacaatt cccagttact gccatcttcc gggttattgg
    18541 tatggtcgtg atcaatgtgg tgaacggtaa gttcacgcag gttagaataa acaaactcgc
    18601 gggaacagcg accgcagacc cacggataga tttttaatgc tttttcgcga tagccgcttt
    18661 ctaaccgtgc atagtttttt gggatgatag ccatgaaaag ttacctggat gaaatgtagg
    18721 gttacgcgca gtttcccaag atgcggggat tttcgcaatc tccatacatc aaaaaaaatc
    18781 ccgatcttct gaccgggatt ttcaacatca acgggaactt gattaaggca gaattgacgg
    18841 ctgatccgcc ccttcttttt ccactttctg ctgcaacatg tgttcgcgct tcatacccag
    18901 tttcagagcc aacgcagatg ctacgtagat ggaagatgca gtgccgatgg aaacaccgat
    18961 aagcatggtc agcgagaagc cttccagtac cggaccaccg aagagataca gcatcaggat
    19021 aaccatcaag gtagtaccgg atgtgatcaa ggtacggtgc agcgtctggg tcaaggacac
    19081 gttaaagatt tcgtaaggcg taccgcgacg gatcttgcgg aagttttcac gaattcggtc
    19141 cgataccacg atactgtcgt taagcgagta accgataacc gacatcaacg atgccacaat
    19201 ggtcaggtca atctcgatat ggaataacga caaaataccc agcgtaatga taacgtcgtg
    19261 cgtcagcgca ataaccaccc ctgccgccag tcgccactca aagcggaaac ctacgtacac
    19321 gaggatagac agcagcgctg ccatcaacgc catcgcaccg gtttgcgcaa ggtcagcccc
    19381 cacgctcgga ccgacgaact caatacgctt cactgctgca ttctgattgg tggattcgtt
    19441 aatcaccttc agaacttggc tgcccagcac ctgaccgccg gtttcgcctt cagcaggcgg
    19501 catacggacc atgatgtcgt ggctgctacc aaagttttgc agcattggct cttcaaaacc
    19561 ggctttttgc aatgcatcac gcattacgtc aatttcagcc ggtttttcga gcgtaatttc
    19621 gataaccgta ccaccggtga aatccagccc ccagttaaag ccgcgcacgc ccataataac
    19681 gatagcagcg attaacagca gaccagagat gccgaaagcc cagtagtccc agcgcataaa
    19741 gtcatagact ttacggccgt ggtttagttg ttcaacagta tattcctgtg ccacatcgca
    19801 ctcctcagat tgacagcttc ttgacgcgct tgccgccata tagcaggttt acgatggcac
    19861 gcgtaccgac aatcgcggta aacatcgacg tcgccacacc gataccggta gtaatcgcga
    19921 accctttaat tgccccggta cccactgcgt acaggatgat gactttaatc agcgtggtga
    19981 tgttcgcatc gaagatagaa ctgaatgcgc cacgataacc ttcatcaatt gcctgttgaa
    20041 cagtacgtcc gttgctcaac tcttctttaa tacgttcgtt gatcagtacg ttcgcatcga
    20101 ccgctaccgc aagggttaag acgatacccg cgatacctgg catactcagt gttgcgcctg
    20161 gcaacagcga cataataccg acgatcaaga tcaagttggc aatcagagca ctggtcgcaa
    20221 tcagaccaaa cttcttatag aagatgatca tgaacagaat agaaaccagc agaccggcaa
    20281 ggcaagcctc cagcccctgc tcaatgttct gcatacccaa ggtcgggcca attgtacgtt
    20341 cttcaacaat ctgaatcggc gcgatcaacg caccggcacg cagcagcagt gacagctgac
    20401 gggcttcgtt cgggttgttg atgccggtga tacggaagct gttacccaga cgagactgga
    20461 tgttggcgat gttaatcacc tcttcctgtt tcaccagaac cgcacgacca tttgcatctt
    20521 tcttaccgct gtctttgtac tccacaaaca gggttgccat cggtttgccg atgttgtcct
    20581 tggtgaagtt agacataatg ttgccaccag cgctatcgag cgagatgtta acctgcggct
    20641 ggttgtattc gtcctggctg gaagtggagt cggtgatatg gtcaccagtc agaattacgc
    20701 gtttgtacag cacaaccggc tgaccttcgc gggtctgttt cacttcagag tcgcccggta
    20761 cgcgaccgga tgccgcagcg gcctgatcaa cattggtgtt taccagacgg aattccagcg
    20821 ttgccgtcgc gcccagaatc tctttcgcac gcgcagtgtc ctgaataccg ggcagttcaa
    20881 caacgatacg gtcagcaccc tgacgctgaa ccaccggctc cgccacgcca agttggttta
    20941 cacggttacg caggatatta atgttctgct gcaccgcata ttcacgcgct tcactcagac
    21001 gagcatcgct cattaccgca cgcagctggt tgctgccctg gctgctaatc accaggtccg
    21061 gatggcgctt gctcagatac gcaatggctt catcacgagc tttagcatcg cggaaagtga
    21121 tgctcaggcc gtagttgttt tctttacgaa cagtggtata cgggatgccc ttttcgcgca
    21181 ggtcactgcg taggctatcg atattttgtt cctggagttt gccaagcgcg gtgtccatat
    21241 ccacttccat caggaagtga acgccgccac gcaggtcaag gccgagcttc atcggctcag
    21301 catgaatagc tgccagccag cgcggcgttg ccggggcaag gttaagcgcc acgacgtatt
    21361 tgtcacccat aacgcccatt aatgcttcac gagcgcgcaa ctgggtgtca gtggagtcga
    21421 agcgcgcaag aatagcgccc tcttccagtg ccacagactt agcagttatt ttttcttctt
    21481 gtaacgtttt ctggacctgg atcagcgttt gctcactggc ggcgactccg cgcgcaccag
    21541 tgatctgaac agccggatcc tcaccaaaca ggttgggaag cgcatacagc agaccgatga
    21601 caatcaccac gatcagcatg acgtacttcc acaaaggata acggtttaac acggcaattc
    21661 ccttagggaa aaattttaat tacagcgcct tcatggtgcc tttcggcagg acggcagcta
    21721 cgaagtcacg tttaataact acttcagtgg tgtcattcag cgcgatagca atgtagccgt
    21781 tttccgctac tttggttacg cgaccaacca ggccaccgtt cgtcagaact tcatcacctt
    21841 tggcaatgga gtccatcagc tttttgtgtt ctttggtgcg cttctgctgt ggacgcagga
    21901 tcatgaaata gaaaatcaga ccgaatacca ccagcatcaa aatcaaagac atcgggctac
    21961 cttgcgccgg tgcacccgtt gccgctaccg catcagaaat aaaaaagctc attaaatttc
    22021 cctcattatt aatattaatc aacgttcaaa ggtggtactt ctcgcccctg acgctggtaa
    22081 aaatcagtta cgaagctctc taatttaccc tcttcaatag ccttgcgtaa acccgccatc
    22141 aaacgctggt agtaacgaag gttatgaatg gtgttgagtc gcgcgcctaa tatttcgttg
    22201 caacggtcga gatgatgcaa gtaagcgcgt gaataattgc gacaggtgta gcaatcacac
    22261 tcaggatcga gtgggccagt atcgctctta tatttcgcat tgcggatttt caccacgcca
    22321 tcggtcacga acaaatgacc atttcgggcg ttgcgggttg gcattacgca gtcaaacata
    22381 tcgataccgc gacgtacgcc ttcaaccagg tcttccggtt taccaacgcc catcaggtaa
    22441 cgcggtttgt ctgccggaat ttgcggacac acatgctcca gaatacggtg catatctgct
    22501 ttcggctcac ccacagccag accgccgaca gcgtagccat caaagccgat atctaccaga
    22561 cctttaacag atatatcacg taaatcttcg taaatactgc cctgaattat accaaataac
    22621 gcatttttgt taccaagact gtcaaaacgc tcacggctac gcttcgccca acgcagagac
    22681 atctccatag agcgttttgc gtaatcccag tcagcaggat acggcgtaca ctcatcaaag
    22741 atcatgacga tatccgaacc aagatcgtac tgaatctcca tcgacttttc cgggtcgagg
    22801 aaaatcgggt cgccgttgat cgggttacgg aagtgaacgc cctgttcggt gattttacga
    22861 atatcaccaa ggctgaagac ctggaagccg ccggagtcgg taagaatcgg tcctttccac
    22921 tgcataaaat cgtgcagatc gccgtgcagt ttcatgattt cctggcccgg gcgcagccac
    22981 aggtggaagg tgttgccgag gataatttgc gcgccagtgg cttcaacttc ttccggcgtc
    23041 atccctttta cggtgccgta ggtgccaaca ggcataaaac aaggcgtttc cactacgcca
    23101 cgatcaaaga ccaggcggcc acggcgtgcg cgaccgtcgg tggtgtccag ttcaaatttc
    23161 attttttctc cactacgtca gaaaaacagt ccaacgttta aaccagcgcc gcggaattac
    23221 tccccgacgc gctcattaat tgcctgcgga ttgtacgtga taaacatcgc atcaccgtaa
    23281 ctaaaaaagc gatatttctc ttctaccgcc gctttatagg cattcatggt gtgttgataa
    23341 ccggcaaagg ccgaaaccag cataatcagc gtcgactctg gcaagtggaa gttcgtcacc
    23401 agcgcatcga ccactttgta ctggaagccc ggatagataa agatttgggt atcgtcaaag
    23461 aacggttcga taagatcgtt ttttgccgcc tgtgccgcgc tttccagcga acgtactgaa
    23521 gtggtgccaa ccgcaatcac ccggttaccg cgcgctttcg ccgccagtac cgcgtctacc
    23581 acatcctgcg gtacttcagc gtattccgag tgcatgatgt gatcttcaat ggtgtcgacg
    23641 cgcaccggct ggaaggtgcc cgcaccaacg tgcaacgtca caaacgccat ctccacgcct
    23701 ttggcgcgta atttttccag caaaggctcg tcaaaatgca gaccagcggt cggggctgca
    23761 accgcgcccg gtttttcgct ataaacggtt tgataaagtt cgcggtcagc gtcttcgtcc
    23821 ggacgatcga tatacggcgg cagcggcata tggccgatgc tgttgaggat atccagtacc
    23881 gagcgttcat cattaaattc gacttcaaac agtgcgccgt ggcgcgcggt cattgttgcg
    23941 ttgatacttt cgtcatcgcc cagcaacagt tctgcgccag gttttggcgc tttcgaggcg
    24001 cgaatatgcg caagaatgcg tttgtcgtcg agcatccgct caaccagcac ttcaatcttg
    24061 ccgccgctgg ctttacgccc aaacagacgc gccgggatca cgcgggtatt attaaaaacc
    24121 agaagatcgc cggggttgag cttatcgagt aaatcggtga aagtaccgtg cgtcagcgcg
    24181 cccgtcggcc cgtccagcga cagtaaacga cagctactgc gttcaggcat gggatagtgg
    24241 gcaatcaggg attcgggcaa ttcaaaggag aaatcggtaa cgcgcatgac actgactcag
    24301 actaaaataa gagtcgggta gtctagtgcc ggggcgctct ccctgcaacc tttacccccc
    24361 tttggataga atataatcga tgaatttttt agctcacctg catttagccc atctcgcgga
    24421 aagctcgctt tctggcaatt tactggctga tttcgtacgc ggaaatcctg aagaaagttt
    24481 tccgcccgac gtcgtggctg gcattcatat gcatcgacgt attgactgac aatctgccgg
    24541 aagtccgcga agcacaggag tggtttcgta gtgaaacgcg ccgcgttgcg cctattacgc
    24601 tggatgtcat gtgggatcac tttctttccc gccactggtc gcagctgtcg ccggactttc
    24661 cgctacagga atttgtctgt tatgcccgcg agcaagtgat gacgattttg ccggactcac
    24721 cgccacgttt tatcaatctg aacaattact tgtggtcaga gcagtggctg gtgcgctatc
    24781 gcgatatgga tttcatccag aacgtgttaa acggcatggc aagccgccgc ccacgtctgg
    24841 atgccctgcg tgactcctgg tacgatttag acgctcatta tgacgccctc gaaacccgct
    24901 tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca caaggcgtta taaaacgtta
    24961 gttcatccat accgtagccg aaatggcagg caaagccaga atcccgtcag tcagttgccc
    25021 atgcccttct ttgcattgcc attgcacggc attgagaaac ggtgacgcgg gtagcaccac
    25081 ttcactggcc tcgccacggt tgattgccac cagtacacgt tgctgattca gcacgcggac
    25141 aaataccact acgttatctt ccgcatacag cacctgacag ccgccacgac gcagcgcctg
    25201 acttttctta cgcagcacaa tcattcgccg gtacagcgcg aataacgccg tatcctgctt
    25261 ttccacctgc caggggaacg gtttacggca aaacggatcg tttttaccat ccagtcccac
    25321 ttcatcaccg taataaatgc acggtacacc aggccaggtg aacagccaga ccaccgccag
    25381 cggcaggcgc gcaatatccc gaccgagcag cgttttaaat cgcgcagtat cgtggctatc
    25441 gagctgatta aacatacgta attgttgttg atgagaaagc cctgcgcggt aattatccat
    25501 ccaggccata caggtttggg cgtcaatttg ctgcggatca taagagatgt cggtattggc
    25561 aagaaatccc cacaacggga atgtgaagcc acgatagttc atggcagcat cttccacatc
    25621 ggcctgtaac cattgccgtg catcgccaaa atgttcgccg acaatatacg cttccggctg
    25681 ggtttctttc gccgcttcgg tgatcccggc aacgtgctgc atattattgc gcgccccacc
    25741 cgcctccccc agcatatgca ccacatccag ccgccagccg tccatattcc acggcgcttt
    25801 cagccagtgg cggacaatac tgtcttcccc gcgataaatt tcattcacca gactttccga
    25861 ctgataatcc agcttcgtca agctggcata gccaagccag tcgagcgccg tgccatcatc
    25921 actaaacgag taccagtcgc gccagggcga ttcagagttg tgacaagctc cccccgtgcc
    25981 aagattgtgc ctgtcaaacc aggcatggga atcgccactg tggttaaaca cgccgtccag
    26041 caccagccgc attcccagct gctgcgtatt gtgtcgcaaa cgcagcaaca ccccatcacc
    26101 gccaaactgc ggatcgacat ggcgataatc ctcggtatcg tatttatgta cgctgggagc
    26161 tttaaacacc ggattgagat acagcgctgt cacgccaagc tttttcagat acggcagttt
    26221 ttcgcttatc ccgtccagat cgccgccata gaacgttgat ccgcccgcct gcgccgtgac
    26281 cggttcatcc cagtcacgca agatgatctc ttgtccggct gcatgatggt aatagacatg
    26341 atcctgttca gcttcacgag gaagactacg cgcaaaacga tcagggaaga tctgataaaa
    26401 aatctgatcc gcagcccatt gtgggccgat atcaggtaca tcgacggcaa actgctccag
    26461 tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac caacgctggc gatcgtgcca
    26521 cagcagtttg aaactgtaac gccgccgggg ttgtccgctg gagagatcaa tcgccgcacg
    26581 ccatgcggtg acgcctggct gcggctgact gcgctgctta tgcattggta ctgacatttc
    26641 ttcgttatcg tgttctgtac gcagcataat gcgctgcggt gggtcttcgc ccgtcagcca
    26701 cagtgtaatg agcagttgat ctttgctttg tttaacaaat gggggcaccg gcaggtgcca
    26761 tgcatttaac atcataattc ccctgtgatg aaaatctgat caccttgcca tagcctaatg
    26821 caggatcact catcatctga aggatatttg ggggaggagg acgggggcgg ataactttcg
    26881 gaatagccgg atcaggattt cctcctgaac cggcttacag aacgcaagta ttattggttt
    26941 tcagccagct gacgatcgtg gcggcgttta aacatccagc caatcaacag cacaacaatc
    27001 cacgcgaaac cgacatacag cgagatacgc gtatccgggt gataaccaat caacccgata
    27061 ataaagagca ggaaaatcag cccgccgatg gtcgttgcta ccccacccgg cactttaaat
    27121 ttcagcgcct taacttcttc tggcggcaaa cggcgacgga aggcgatttg cgacagcagg
    27181 atcataatcc acacccatac cgtggcgaag gttgccagcg aagcgatcac caggaagacg
    27241 ttttccggca tgatgtagtt cagatacacc gcaaacagca gcgcggtagt catcaccagc
    27301 accgtaaccc acggaatacc gcgacgcgac gttttgctga aaattttcgg tgcgctgccc
    27361 tgctctgcca taccgtggag catacggcct acgccaaata cgtcactgtt aattgccgac
    27421 agcgaagcgg tcagcacaac aaagttaaga atgctggcgg caaaggtaat gcccatatgc
    27481 tggaacgtca gtacgaacgg gctaccggca gtgccaacct gattccacgg gtagatagac
    27541 ataatgacga acagcgtacc gacgtagaac accagaatac gcatcggcac ggagttaatc
    27601 gcacgcggaa tcgatttctc aggatctttc gcttcaccgg cggtaatccc gataatttcg
    27661 atcccaccgt aagcaaacat caccatttgc aacgacatta ccatgccaag ccagccgtta
    27721 ctgaagaagc cgccgttgct ccacaggtta tgaataccgg tcggttgccc gccgttgcca
    27781 atcccccaga tgatgatgcc gaaaccggcg acaatcatga tgatgatggt ggcgacttta
    27841 aagaacgaga accagaattc cagctcaccg aagaccttca cgctcatcag gtttacggcg
    27901 caaatgatca gcaccacgct cagtacccaa atccagtgcg gcaccgtcgg gaacaagaca
    27961 cccatataga taccaaaagc ggtcacatcg gcgatggcga caataaggat ttcaaaacag
    28021 taggtccatc cggtaatgta acctgccagc gggccaaggt tttcctgcgc ataacgcgag
    28081 aaagagctgg cggccgggtt atgtaccgac atttccccca gcgcacgcat aatgatatac
    28141 gccgcgatac caccgataat ataggccaac aacacgctcg gaccggccat tttgatggcg
    28201 tctgccgaac cgtaaaacag cccggtgcca attgctgaac ccagtgccat aaagcgtatg
    28261 tggcgggtac ttagcccacg ctttagctta ttcttacttt ccatcaattc ctaacccatc
    28321 aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt aaaacaaatg ttcagtgatt
    28381 tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat cccagataat ggccagaacc
    28441 accatcacca ctgttggcat taaccacgcc agaccttgtt cggccagcgg taaacgctgc
    28501 gcccaggacg gtaagatatc gctgaatgca gatgctttga tcccgtcgag aataccaaaa
    28561 agcaggctga taaacatcgg cggagcaatc acgcgggacg aattatgcca ccatgagcgt
    28621 gtaaaactta atacaaccag tgcgatacac ggcggataaa tagcggtcag caccggtacg
    28681 gagatctgga tcagctggct taagccgagg ttagacacca ccatcgagaa gccgccgagg
    28741 ataaacacca gcgtacgata agagagcggt acgtactggg caaagaattc tgcacaagca
    28801 caggtcaggc caaccgccgt gaccagacag gcgatgaaga ttaacgccgc cagcaggaag
    28861 ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca gaatagcagc gccgtttgca
    28921 gactgatcga ccagcgacgc gctgtctgaa cccagacaga acagcgccag gtacagcaaa
    28981 gtcagaccaa cacccgccat caggccagcc cagacggtat aacgggtcag cagacgcgct
    29041 tcggtaacgc cacgagaacg cgccgcgtta acaataacga taccaaacac cattgcgccc
    29101 agcgtatcca tggtcagata gccgttaacg aagccgttag aaaatgcagc gttttgataa
    29161 gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa tcgcgacaac agacaggatg
    29221 accagcgcga taattttcag cggcgcaagg aagttgccca cggtatccag cagcttgccc
    29281 ggatagagcg aaaccagaat aacgatagcg aaatagacca ggctgtagat aaacagcggc
    29341 agcgcggaat cacccgtcag cggcgcaatc cccacttcaa aggaaacggt agctgtacgc
    29401 ggcgtagcga aaagcggccc caccgccagg taacaaaccg ttgccagcag tacgccagcg
    29461 actttaccga ttggcgtgct gaggctgtca acaccgccgc caacttttgc cagcgccact
    29521 accgttaata ccggcaggcc aacggcagta atgaggaagc cgaatgccgc agtccagacg
    29581 tgttcgcctg cctgtaagcc gaccattgga gggaaaataa tgttacctgc gccgacgaac
    29641 aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg atcttaattg atgggtcata
    29701 aatcatactg cctgtggatg tggtgttgta acgttaaatt tttgtgccaa tccctgggga
    29761 tgatacagcg gtatatttaa acaattttag cgggaaatac tcccaaccag gcgcggcgaa
    29821 gaatagtttt tcgatttacc gcgttaatgc agtcagtatg acagcacctg aagcgcaatt
    29881 taaacgctta taacgtttta aagcaaggag ggatctaaaa accagaggta tatagcgata
    29941 agaaatcacc aaccagtata aaacactatt tccgtgaaaa agacatggct aatcatgcga
    30001 acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat tttgactgca aagaaaataa
    30061 gccagcatcg gctggcttat ggcaataaaa gatgacaaag gcggattaat cgctgttttt
    30121 ggcaattaaa cgttccggga taacaaaact gaaacgtgtt ccttttccta ctgtactctc
    30181 aatattcagg cgactttcgt gatgattcac cgcatgtttc acgatcgcta accctaatcc
    30241 gctaccgccg gtttgccggg aacgcgcttt atcaacgcga taaaaacgct cggtcaggcg
    30301 cggaatatgc tccggtgcaa tgcccggtcc gttatcttca acgctaaatt cggcaccgtg
    30361 cggcactcgc tgccagcgta cggtgatatg cgtgccttcc ggcgtatgat tcacggcgtt
    30421 atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg ttgccagaca ccttgaggcc
    30481 gttatctatc tcaaaggtaa atgtctgttt tttctgactc agagtctgag cctcgcgctc
    30541 aacaacgcgc agcatcatcg gcacatcaac cttttcattg agcaattgcg tcggcgcggc
    30601 ttcaattttc gacagcgtta gcaactgctt caccagccct tccatccgct gggtctgctc
    30661 gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc agcggctgct catccatcat
    30721 ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac tcatggctca cgttggcaaa
    30781 aaagttacgc cgcgcccctt ccagttgatg catttgcgtg acatcacgcg ccaccatcag
    30841 caactgtttg tgggtataag gcatgacgcg aatttccaga tgccgcccgg tgttgagcac
    30901 cagattgagc gggcgagaaa aatcacgcgt tttcagatat tgcgtaaact ccgggtaacg
    30961 cagtaggtta aggatgttct gcccgttatc ttccggccag cgcaaaccaa gaatttgttg
    31021 cgccagaccg ttacaccaga aaataccgcc ctcttccgtg gtcagcacca ccgcgtcggg
    31081 cagcgactcc gcgccgctac gaaagcgttt aatcagattg cccagttcac ggcggcgttt
    31141 tttatttcgc agctgcatct ggtgtaagcc gtatagtagc ggttcccagc taccacgccc
    31201 cggtggcggg gtcatactgc gatccaccca cagccaccat gaaaggcgca ataaattcca
    31261 gaaatgccag ataagcagtc ctgttaccga tgccagcaaa aaccagggca ggtaaccaaa
    31321 aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc agctccagca ccagcctttt
    31381 ccacgacagc cgttccagca cgtaagatac tccagttaag aaatcataag ccctgctctg
    31441 cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga tatcctgtac cgcgcacggt
    31501 ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta cgcaggcgac gaatgtggac
    31561 atcgaccgtg cggtcttcca catacacgtt agttccccag acgtggttta acagctgctc
    31621 gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc agcagtttaa attctgtcgg
    31681 ccccatctcc agcggctctt cgcccgccat cactcggtga gatgtcgggt cgagacttaa
    31741 tccctgcatc ttaatcacct cttccaccgc cattggcgaa atacggcgca ttaccgcttt
    31801 gattcgcgcc accagctcct tcggcgaaaa tggcttggtg atatagtcat ccgcgccggt
    31861 ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg gtcaacatca ccactggaat
    31921 atcccgggtc atcgactcgc gcttgaggtg tttgatgaac tggataccgg agccgccagg
    31981 taacatccag tcaaggagaa ttaaatccgg ctagggttca ttcagttgat tcacagcact
    32041 gtcataatct tccgcttcga ccggctgaaa gccattttgt tcgagcacga agcagaccat
    32101 ttcgcgaatt ggagcttcat cttctacgac cagaatacgt ctcgccatga tttgccctgt
    32161 tgtaataaat aggttgcgat cattaatgcg acgtcattat gcgtcagatt tatgacagat
    32221 ttatgaaaag ctcgtcgcac atatcttcag gttattgatt tccgtggcgc agaaaaaagc
    32281 aaatggcaca tctgtttggg tataatcgcg accatgcttt ttcgccaggg aaccgttatg
    32341 cgcatccttc acacctcaga ctggcatctc ggccagaact tctacagtaa aagccgcgaa
    32401 gctgaacatc aggcttttct tgactggctg ctggagacag cacaaaccca tcagatggat
    32461 gcgattattg ttgctggtga tgttttcgat accggctcgc cgcccagtta cgcccgcacg
    32521 ttatacaacc gttttgttgt caatttacag caaactggct gtcatctggt ggtactggca
    32581 ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg atatcatggc gttcctcaat
    32641 acaaccgtgg tcgccagcgc cggacatgcg ccgcaaatct tgcctcgtcg cgacgggacg
    32701 ccaggcgcag tgctgtgccc cattccgttt ttacgcccgc gtgacattat ttccagccag
    32761 gcggggctta acggtattga aaaacagcag catttactgg cagcgattac cgattattac
    32821 caacaacact atgccgatgc ctgcaaactg cgcggcgatc agcctctgcc catcatcgcc
    32881 acgggacatt taacgaccgt gggttccagt aaaagtgacg ccgtgcgtga catttatatt
    32941 ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag ccgactacat cgcgctcggg
    33001 catattcacc gcgcacagat tattggcggc atggaacatg ttcgctattg cggttccccc
    33061 attccactga gttttgatga atgcggtaag agtaaatatg tccatctggt gacattttca
    33121 aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg taactcaacc catggcggtg
    33181 ctgaaaggcg atctggcgtc gattaccgca cagctggaac agtggcgcga tgtatcgcag
    33241 gagccacctg tctggctgga tatcgaaatc actactgatg agtatctgca tgatattcag
    33301 cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag tattgctggt acgtcggagt
    33361 cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg aaaccctcag cgaactcagc
    33421 gtcgaagagg tgttcaatcg ccgtctggca ctggaagaac tggatgaatc gcagcagcaa
    33481 cgtctgcagc atcttttcac cacgacgttg cgtaccctcg ccggagaaca cgaagcatga
    33541 aaattctcag cctgcgcctg aaaaacctga actcattaaa aggcgaatgg aagattgatt
    33601 tcacccgcga gccgttcgcc agcaacgggc tgtttgccat taccggccca accggtgcgg
    33661 ggaaaaccac cctgctggac gccatttgtc tggcgctgta tcacgaaact ccgcgtctct
    33721 ctaacgtttc acaatcgcaa aatgatctca tgacccgcga taccgctgaa tgtctggcgg
    33781 aggtggagtt tgaagtgaaa ggtgaagcgt atcgcgcgtt ctggagccag aatcgggcgc
    33841 gtaaccagcc ggacggtaat ttgcaggtgc ctcgcgtaga gctggcgcgc tgcgccgacg
    33901 gcaaaattct cgccgacaaa gtgaaagata agctggaact gacagcgacg ttaaccgggc
    33961 tggattacgg gcgcttcacc cgttcgatgc tgctttcgca ggggcaattt gctgccttcc
    34021 tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga gttaaccggc actgaaatct
    34081 acgggcaaat ctcggcgatg gtttttgagc agcacaaatc ggcccgcact gagctggaga
    34141 agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc ggaacaagtg caatcgctga
    34201 cagcgagttt gcaggtactt actgacgaag aaaaacagtt aattaccgcg cagcagcaag
    34261 aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt gcagcaagaa gccagccgcc
    34321 gtcagcaggc cttgcaacag gcgttagccg aagaagaaaa agcgcaacct caactggcgg
    34381 cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca ctgggaacgc atcgcagaac
    34441 acagcgcggc gctggcgcat attcgccagc agattgaaga agtaaatact cgcttacaga
    34501 gcacaatggc gcttcgcgcg agcattcgcc accacgcggc gaagcagtca gcagaattac
    34561 agcagcagca acaaagcctg aatacctggt tacaggaaca cgaccgcttc cgtcagtgga
    34621 acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca aaccagcgat cgcgagcatc
    34681 tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa acttaatgcg cttgcggcga
    34741 tcacgttgac gttaaccgcc gatgaagttg ctaccgccct ggcgcaacat gccgagcaac
    34801 gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat tgttccccaa caaaaacgtc
    34861 tggcgcagtt aatcgtcact atccagaatg tcactctgga acagacgcaa cgtaatgtcg
    34921 cacttaacga aatgcgccag cgttataaag aaaagacgca gcaacttgcc gatgtgaaaa
    34981 ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc ccagcgtgca cagttacagg
    35041 cgggtcagcc ttgtccactt tgtggttcca ccagccaccc ggcggtcgag gcgtatcagg
    35101 cgctggagcc tggtgttaat cagtctcgat tactggcgct ggaaaacgaa gttaaaaagc
    35161 tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc attaacgaag cagcttcagc
    35221 gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca agcacttact caacaatggc
    35281 aagccgtcac ggccagcctc aatatcacct tgcagccaca ggacgatatt caaccgtggc
    35341 tggatgcaca agatgagcac aaacgccagc tgcggttact cagccaacga catgaattac
    35401 aagggcagat tgccgcgcat aatcagcaaa ttatccagta tcaacagcaa attgaacaac
    35461 gtcagcagca gctcttaacg gcattggcgg gttatgcact gacattgcca caggaagatg
    35521 aagaagagag ctggttggcg acacgtcagc aagaagcgca gagctggcag caacgccaga
    35581 acgaattaac cgcgctgcaa aaccgtattc agcagctgac accgattctg gaaacgttgc
    35641 cgcaaagtga tgatctcccg cacagcgaag aaactgtggc gctggataac tggcggcagg
    35701 ttcatgaaca atgtctcgca ttacacagcc agcagcagac gttacagcaa caggatgttc
    35761 tggcggcgca aagtctgcaa aaagcccaag cgcagtttga caccgcgcta caggccagcg
    35821 tctttgacga tcagcaggcg ttccttgcgg cgctaatgga tgaacaaaca ctaacgcagc
    35881 tggaacagct caagcagaat ctggaaaacc agcgccgtca ggcgcaaacg ctggtcactc
    35941 agacagcaga aacgctggca cagcatcaac aacaccgacc tgacgggttg gctctcactg
    36001 tgacggtgga gcagattcag caagagttag cgcaaactca ccaaaagttg cgtgaaaaca
    36061 ccacgagtca aggcgagatt cgccagcagc tgaagcagga tgcagataac cgtcagcaac
    36121 aacaaacctt aatgcagcaa attgctcaaa tgacgcagca ggttgaggac tggggatatc
    36181 tgaattcgct aataggttcc aaagagggcg ataaattccg caagtttgcc caggggctga
    36241 cgctggataa tttagtccat ctcgctaatc agcaacttac ccggctgcac gggcgctatc
    36301 tgttacagcg caaagccagc gaggcgctgg aagtcgaggt tgttgatacc tggcaggcag
    36361 atgcggtacg cgatacccgt accctttccg gcggcgaaag tttcctcgtt agtctggcgc
    36421 tggcgctggc gctttcggat ctggtcagcc ataaaacacg tattgactcg ctgttccttg
    36481 atgaaggttt tggcacgctg gatagcgaaa cgctggatac cgcccttgat gcgctggatg
    36541 ccctgaacgc cagtggcaaa accatcggtg tgattagcca cgtagaagcg atgaaagagc
    36601 gtattccggt gcagatcaaa gtgaaaaaga tcaacggcct gggctacagc aaactggaaa
    36661 gtacgtttgc agtgaaataa ctattcagca ggataatgaa tacagagggg tgaattatct
    36721 cttggccttg ctggtcgtta tcctgcaagc tatcacttta ttggctacgg tgattggtag
    36781 ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt ttatctttgg ctctgggcac
    36841 gtttggtttg gggatggccg aatttggcat tatgagcgtg ctcacggagc tggcgcataa
    36901 cgtaggaatt tcgattcctg ctgccgggca tatgatctcg tattatgcac tggtggtggt
    36961 ggtcggtgcg ccaatcatcg cactcttttc cagccgctac tcactcaaac atatcttgtt
    37021 gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc acgctctctt cgtcttacct
    37081 gatgctcgcc attggtcggc tggtatccgg ctttccgcat ggcgcatttt ttggcgtcgg
    37141 agcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc accgccgccg tggcggggat
    37201 ggtttccggg atgacagtcg ccaatttgct gggcattccg ctgggaacgt atttaagtca
    37261 ggaatgctgg cgttacacct ttttattgat cgctgttttt aatattgcgg tgatggcatc
    37321 ggtctatttt tgggtgccag atattcgcga cgaggcgaaa ggaaatctgc gcgaacaatt
    37381 tcactttttg cgcagcccgg ccccgtggtt aattttcgcc gccacgatgt ttggcaacgc
    37441 aggtgtgttt gcctggttca gctacgtaaa gccatacatg atgtttattt ccggtttttc
    37501 ggaaacggcg atgaccttta ttatgatgtt agttgggcta gggatggtgc tgggaaatat
    37561 gctaagtggc aggatttcag gacgttattc accactgcgc attgcagcag tgactgactt
    37621 tatcattgta ctggcactgc tgatgctctt tttctgcggc ggcatgaaaa caacgtcgct
    37681 tatttttgct tttatttgtt gcgcgggatt atttgccctt tcagcacctc tgcaaatatt
    37741 gttactgcaa aatgccaaag gaggagagtt attaggtggc gcaggtgggc aaatagcgtt
    37801 taacctcggt agcgccgtcg gcgcatattg cggaggtatg atgctgacgc tggggctggc
    37861 atataattac gtggcgctgc ctgccgccct gctttcgttt gctgcgatgt catcgttgct
    37921 gctgtatggt cgctataagc gccagcaagc ggcggatagt ccggtgctgg cgaaaccact
    37981 ggggtagatt actgtcacgg tgcgcgttgc ttattgccgg atgcggcgtg aacgccttat
    38041 ccgccctacg cggttctggc acattttgta ggcctgataa gacgcggcaa gcgtcgcatc
    38101 aggcatgatg cgccaattgc ctacgttttt tactcttgtg gccataacca cgcagcgccg
    38161 cgtacgccgc tggaatcacc gtgcttcgcc ttactcaccg gcgtttcaca ttcgccgccg
    38221 aagacaaatt gtttaatcaa ctgcccaacc gtttgatata aacggtctac attgctcatc
    38281 ccgcccccca ggacaatcac atccggatcg agaatattca cgacatgtgc cagcgatttt
    38341 gccagccgca gctcgtagcg acgcaatgcc agttccgcta ccggatcgct ttcttcaacc
    38401 aggcggataa tttcactgcc tttcagcgca tgtccgctca aacgacgata atccgtcgcg
    38461 aatcccgtgc ccgaaataaa ggtttcaata caaccttgtt taccgcaata acaagggact
    38521 tcctcgcgat aacgcagttc gtcttcgtcc atccacggta gcggattgtg tccccactca
    38581 cctgccgtgc cattgccgcc gatatgcgtc cgcccattga atgccacgcc cgcgccgcat
    38641 cccgtgccga tagtcacggc aaataccgtc tgcgctcccg ctgccgcgcc atctactgct
    38701 tctgaaaccg ccagacagtt ggcatcattt gccagccgca cttcccgctg caacctcgcg
    38761 cttaagtctt tatcgaatgg ctgaccgttg agccaggttg aattgacatt cttcaccaca
    38821 ccggtgtagg gcgaaatcga acctggaatg cccataccta ccgttccgcg ctgccctgtc
    38881 gcctgctccg ccatatcaac caacgtggcg atcgtttcaa tagtctgccg gtaatcatca
    38941 cgcggcgtgg gcagacgatg gcggtacaac tgctcccctg catcgcccag tgcaatcact
    39001 tcagttttgg tgccgcctaa atcgatacct atacgcactg tactctcctt atttttttca
    39061 atatcaatag cgtagagacg gacaaccgga ttggcaatgc aaggccgccg acaattcgtt
    39121 atcatgcccg ctaaatttaa cgacaaggcc gtggaaatta tcatgctgtg gttcaaaaat
    39181 ttaatggttt accgtcttag ccgcgagatt tcgctgcgtg cagaagagat ggaaaaacag
    39241 ctagcctcga tggcatttac cccatgcggc agccaggaca tggcgaagat gggctgggtt
    39301 cctccgatgg gatcgcacag cgatgcgtta acgcacgttg ccaatggtca aattgttatc
    39361 tgcgcgcgca aagaagaaaa aatcctcccg tctccggtga ttaaacaggc gctggaagcg
    39421 aaaatcgcca aactggaagc ggaacaggcg cgtaagctga agaaaaccga aaaagattcg
    39481 ctgaaagacg aagtgctgca ctctctgctg ccgcgtgctt tcagccgttt tagccagaca
    39541 atgatgtggc tcgacacggt taacggtttg attatggtgg actgcgccag tgccaaaaaa
    39601 gcggaagata cgctggcatt actgcgtaaa agcctggggt cgttaccggt tgtaccattg
    39661 agcatggaaa acccgattga actgacgctg accgaatggg ttcgctccgg tagtgcggca
    39721 cagggcttcc agctgcttga tgaagccgag ctgaaatcgt tgctggaaga tggcggcgtg
    39781 atccgcgcga agaaacaaga tctgaccagc gaagagatca ccaatcacat tgaagccgga
    39841 aaagtggtga ctaaactggc gctcgactgg cagcagcgca ttcagtttgt gatgtgcgac
    39901 gatggttcgc tcaagcgtct taagttctgc gacgagctgc gcgatcaaaa cgaagatatc
    39961 gaccgtgaag atttcgccca gcgttttgat gccgatttca tcctgatgac tggtgaactg
    40021 gcagcgttaa ttcaaaacct gattgaagga ttaggtggcg aagcacaacg ttaattgctg
    40081 attttccttt aatgccggat gcgacgcctg ccgcgtctta tccggcgtac gaagccacac
    40141 caagcatata attattcgct acggcgagca ataattttta gcgcagcaat attatgcgtt
    40201 ttacgccgta actgatctaa tcgcctcatc cctattcgcc tgtcagcagc acgcagaata
    40261 ttcactaacc cccatttatc gctggtaaat tgttttgtta acagctccgc ttttggggta
    40321 tcaatatatt cacgcaataa atcagaaatt gaacaaatat ccgtcaggta aggataccag
    40381 caagtttcac cgtgaaaatt acgcactcca gcgttacctg caataaagtc tttcgggtaa
    40441 tcccagatca cgtttttatc caacgtgatc caataacgcg gtaaatcggt acttccacca
    40501 ttttggctgc gcatgggata acgggtacag tgaatttgca ggttgatcgt tggcgtcagt
    40561 aagtcataga tctcccgctg taacttgctc catggacgtt gtgtcattgt ttttcctcaa
    40621 gccggtgacg cgcttcactg cgaattttcc gcgttgcccc ggttttcact aaatcacgta
    40681 actgcgaaag atcgggttat tcgacaaata agagtaatga tggatcctct tttaaccacg
    40741 ccgttttcac atcggcagcc agctgtggat tattaatagc cagcgaccga tcctgaggct
    40801 cagtcaatgt cgttaacaac gacgccgggg tatgtgcgtt gcgtaatgcc gtttggcgca
    40861 aatgatcatt gcgactttgc agcaatgcac gccaccattc atcatctgat ttatctttta
    40921 tcgtagccag ttccgcaaaa aatacctgga gatcttcact gatatttata tcaaccttga
    40981 tgaattatga atatatcgga aattgaaaaa atcgcagcaa aggaaatatt attatgggtt
    41041 aaagagagga tatttcagaa aaatgccctc ccgttcaagg ggaaggcgag gaattacaga
    41101 tagcggcaca gataagaggt aggttcggca acttgaagat gaaactcact gtgaccggga
    41161 acattaaaca ccgaaccggc ttcatacact tgccagtcgg tcgcgtcagg cagtaacaca
    41221 ttcagcgcgc cactgattac cgtcatctct tccggctcag cggtgctgaa ggtgtattcg
    41281 ccttcaacca taacacccac gctggcgcga ccagtgctgc tgctggaaaa gccgatcgat
    41341 ttcactttgc cggaaaagta ctcattactt tgaagcataa actggccctg tctggtagtt
    41401 gaacatctac ttcactatag gggccagagg cgctggctgt cacgcaaaat tacaccagta
    41461 attccgcagc cagccgtgca atcaatacgt tagagagcaa gacaggaaca tcgagctgtt
    41521 tttgcagtaa atcgcgatga cgttggtgaa atcctaaaca atccagcatg atgacatcag
    41581 ccccttttgc cagtaattct ttcccagcat caatgatttt ttgttctgaa tcatgaatgg
    41641 ggttacccaa tgaaaatacc ggcgatttct gcaaaatttg ccatttttgc gcctgaacgg
    41701 gcagcatctc ctcaaccgga acgataaccc ccacctgatg atcttcaaca atagaggaaa
    41761 ccagtggagg caatattcgc gacggctcaa gaaagatcgt attacgcgca gtcatactac
    41821 taatgtttgc tgtactcatt aataaaatga cgtcataacc ctgattatcg agcacttcaa
    41881 ccacaccttg cagatctcgc tccactttac gacgcgaaac atgggccagc tggttgtcat
    41941 ttaataatgt gagaatggtg tcttcgcctg cttctggcgc gtactccgcc atcacttctt
    42001 cacgacttaa cttccccagc aggctatgat gggaaatatt atcttcgtca atgtattccg
    42061 tcaggagcgg caaaacctcc tgcataggta caatgccgat ggtcaggatc gccaacgacg
    42121 cactcattgt tcatccacct tacttttctt tcgtcgttaa ttaccgggca agtgtgaagc
    42181 accactgctg acacttactt gtgctacaaa tgacaaaaag cgtagcagca gacgtgcacg
    42241 gcataatgta aagattccaa atgattccag taatggaatg ttattgttta atattctaat
    42301 tattagaaaa acataaatta tgaaaaatgt gacgcagatc ccttgtagtg gcactcagaa
    42361 tcccttccga gcagtctgat gcggtcacta tttgtcaggg tcctcatagc gttttttcgc
    42421 atccatcgct tcctgagccg tcggatgttc actgatcaac gagtctggtt taggatgatc
    42481 ggctctgagt tggtaccagg ttaccgtctg tcctggcttt cctttttcaa tcgtcactat
    42541 atatgcttca cgaggataag gcggtttcgt tggcatcgtt cttccttatt tcacgggatg
    42601 aacgttaagt ataggcgctc taaaatcaac aattgatcgt ctgtgccagg gcgctacgaa
    42661 tttcagaaat cacctggctg ggttcgtttg ttgcgtcgat gataatatgc gcaacttcgc
    42721 gatatagcgc atcgcgttct tccagcactt cctgaacttc ttcgctcagc ggttttcccg
    42781 ttaaggttgg ccgtaaatct tcttccggtg cagcttgcag tcggttaacc aggactgata
    42841 ctggcgcaca caaataaacc acgatcccgt tattttgcat gaagtgacga ttaaattccg
    42901 tcagaataat gccgccgcct gtagcgataa cggtggatgc cgcagttacc gcttccagcg
    42961 ccgccgtttc tctggcgcga aatcccgccc actcttccct ttcgacgatc tccgcgaccg
    43021 tcatattgag ctgtgattgc aaccactgat cggtatcgac aaaccgacgg ttaagcgaat
    43081 cggcaagggc cattccgacc gttgttttac cacagccccg aggcccgatc agaaaaagag
    43141 gttgtgtcat cgtgggtttt ccccaatagg tcgcaatgcg gcgaaagccg gtgtcatgag
    43201 aatagcgatc ataccatcaa actagtacaa tttcgattgt aaagaaaaaa ttccacttaa
    43261 agtaaaaatc tcaatacatc ccttactata ccaataaata ttcaagaatg aagtgtaaat
    43321 aataaattac atttagccac gactacgttg cacttccagc caccacttct ccagctccgc
    43381 ggcaaaggcc tggcggtcac gttgtgaaag gctatctggt ccgccggtct ggatcccact
    43441 ggcacgtaag gtatccataa aatcgcgcat cgtcaggcgc tcacgaatgg tcgctggcgt
    43501 gtaacgttcg ccgcgcgaat taagcgccgc tgcgcctttc tcgatggctt cagcagccaa
    43561 aggtatatct gcggtgatca ccaaatcgcc cgcttcacac tgccggacaa tttcgttatc
    43621 ggcaacgtcg aaacctgccg cgacgcgcag cgtacgaata aatcgcgatg gcggcacgcg
    43681 taaactctgg tttgctacca gtaccagcgg catctgcata cgttccgccg cgagatacaa
    43741 aatctcttta attacattgg gacacgcgtc ggcatccacc caaattgtca taaagtcatc
    43801 ctttgttagg taatcctcta ttgtgtcgcg cttttgcctt ccggcatagt tctgtttatg
    43861 cttctgccag cgattatcaa aacaatgaat ttcacggcag gagtgaggca atggaaaaga
    43921 aaatcggttt tattggctgc ggcaatatgg gaaaagccat tctcggcggt ctgattgcca
    43981 gcggtcaggt gcttccaggg caaatctggg tatacacccc ctccccggat aaagtcgccg
    44041 ccctgcatga ccagttcggc atcaacgccg cagaatcggc gcaagaagtg gcgcaaatcg
    44101 ccgacatcat ttttgctgcc gttaaacctg gcatcatgat taaagtgctt agcgaaatca
    44161 cctccagcct gaataaagac tctctggtcg tttctattgc tgcaggtgtc acgctcgacc
    44221 agcttgcccg cgcgctgggc catgaccgga aaattatccg cgccatgccg aacactcccg
    44281 cgctggttaa tgccgggatg acctccgtaa cgccaaacgc gctggtaacc ccagaagata
    44341 ccgctgatgt gctgaatatt ttccgctgct ttggcgaagc ggaagtaatt gctgagccga
    44401 tgatccaccc ggtggtcggt gtgagcggtt cttcgccagc ctacgtattt atgtttatcg
    44461 aagcgatggc cgacgccgcc gtgctgggcg ggatgccacg cgcccaggcg tataaatttg
    44521 ccgctcaggc ggtaatgggt tccgcaaaaa tggtgctgga aacgggagaa catccggggg
    44581 cactgaaaga tatggtctgc tcaccgggag gcaccaccat tgaagcggta cgcgtactgg
    44641 aagagaaagg cttccgtgcc gcagtgatcg aagcgatgac gaagtgtatg gaaaaatcag
    44701 aaaaactcag caaatcctga tgactttcgc cggacgtcag gccgccactt cggtgcggtt
    44761 acgtccggct ttctttgctt tgtaaagcgc caaatctgcc gatttcaacc actcacgata
    44821 gtgactcatt tgtgggttca gcggcgcaac ccccacacta atccgtaaag ttacctgtgg
    44881 cgtattcggc aagcgtaacg tgtttagccc ttcacgcacc cgtaacatgg cggtaatggc
    44941 gctctcagct ggcgtaccgg acatgattac tgcaaactca tcgccgccaa accgaccaat
    45001 cacatcgcta ccgcgcaggg taatttgtaa ctgtcgggta agcgccacaa tcgcttcatc
    45061 gcccacatca tgaccccagg tatcgttgat gctcttgaaa tggtcgatat cgataatcag
    45121 taacgttgca tcgcgattat gccgccgaca gttatcaaat tcattgcgta acatagtttc
    45181 ccaatgacgt cggttataca cgcccgtcat gccgtcgcga gtactcatga cctgcaacct
    45241 gcgtttatgt tccgccagtt tggttgccgt ctggtagctg acccagccaa acatcagagg
    45301 ataaacgaca ataatgggaa gggagagcca ccattccagc ggagcactat tgaacgacac
    45361 ggtaatgccc gtaagctcga gggtgacaag gcaggaaacc accatcaaca ccagacccgc
    45421 gacaaacaga cgggggccgc ctgcccccat caaattcaga cacagaatca tcaacatagc
    45481 ggtggaaggc agcacgttta cgcccattac gcctacccac attcccgcta atactgcatc
    45541 ggtttttaag ttgtaaattt cccggctaag cggatcgacg gccctgctcg ctatctgcca
    45601 ggctaaatgc ggccagacga acgcccagcc gaccaacacc agccaccacc agcccggcgg
    45661 cgggtgcgaa accagcgttg aagcaatcgg taagaacatg ccagccaggc caaccgcacg
    45721 gggtagtctg acgcgacggg cgaagcgcag cccggaccgc tgatgttcgt tttgaggagt
    45781 taaaggaggt tcctccccat gcgccgccgc ttttttgaaa aagttttcat cattcattat
    45841 ttttgggaac attcagaaac aattttccca aattatagag acggggctaa aggcagaagt
    45901 atgaaatttc gggtgagcca ctttcgtagc tcacccgtta gcacta
//
LOCUS       DAEEUE010000026        40803 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.026, whole genome shotgun sequence.
ACCESSION   DAEEUE010000026 DAEEUE010000000
VERSION     DAEEUE010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40803
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.026"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(181..858)
                     /gene="narI"
                     /locus_tag="KLK84_001505"
     CDS             complement(181..858)
                     /gene="narI"
                     /locus_tag="KLK84_001505"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HBE2008879.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(858..1568)
                     /gene="narJ"
                     /locus_tag="KLK84_001506"
     CDS             complement(858..1568)
                     /gene="narJ"
                     /locus_tag="KLK84_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HBE2008880.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(1565..3103)
                     /gene="narH"
                     /locus_tag="KLK84_001507"
     CDS             complement(1565..3103)
                     /gene="narH"
                     /locus_tag="KLK84_001507"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HBE2008881.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(3100..6843)
                     /locus_tag="KLK84_001508"
     CDS             complement(3100..6843)
                     /locus_tag="KLK84_001508"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HBE2008882.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(7236..8627)
                     /gene="narK"
                     /locus_tag="KLK84_001509"
     CDS             complement(7236..8627)
                     /gene="narK"
                     /locus_tag="KLK84_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HBE2008883.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            8966..10762
                     /gene="narX"
                     /locus_tag="KLK84_001510"
     CDS             8966..10762
                     /gene="narX"
                     /locus_tag="KLK84_001510"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HBE2008884.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            10755..11405
                     /gene="narL"
                     /locus_tag="KLK84_001511"
     CDS             10755..11405
                     /gene="narL"
                     /locus_tag="KLK84_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HBE2008885.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(11406..12800)
                     /gene="ychO"
                     /locus_tag="KLK84_001512"
     CDS             complement(11406..12800)
                     /gene="ychO"
                     /locus_tag="KLK84_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HBE2008886.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            12985..13338
                     /gene="ychN"
                     /locus_tag="KLK84_001513"
     CDS             12985..13338
                     /gene="ychN"
                     /locus_tag="KLK84_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HBE2008887.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(13382..14077)
                     /gene="chaC"
                     /locus_tag="KLK84_001514"
     CDS             complement(13382..14077)
                     /gene="chaC"
                     /locus_tag="KLK84_001514"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HBE2008888.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(14235..14465)
                     /gene="chaB"
                     /locus_tag="KLK84_001515"
     CDS             complement(14235..14465)
                     /gene="chaB"
                     /locus_tag="KLK84_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HBE2008889.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            14735..15835
                     /gene="chaA"
                     /locus_tag="KLK84_001516"
     CDS             14735..15835
                     /gene="chaA"
                     /locus_tag="KLK84_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HBE2008890.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            16240..16347
                     /locus_tag="KLK84_001517"
     CDS             16240..16347
                     /locus_tag="KLK84_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBE2008891.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(16496..17350)
                     /gene="kdsA"
                     /locus_tag="KLK84_001518"
     CDS             complement(16496..17350)
                     /gene="kdsA"
                     /locus_tag="KLK84_001518"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HBE2008892.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(17386..18195)
                     /locus_tag="KLK84_001519"
     CDS             complement(17386..18195)
                     /locus_tag="KLK84_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HBE2008893.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(18199..18591)
                     /locus_tag="KLK84_001520"
     CDS             complement(18199..18591)
                     /locus_tag="KLK84_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HBE2008894.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(18588..19421)
                     /gene="prmC"
                     /locus_tag="KLK84_001521"
     CDS             complement(18588..19421)
                     /gene="prmC"
                     /locus_tag="KLK84_001521"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBE2008895.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(19421..20503)
                     /gene="prfA"
                     /locus_tag="KLK84_001522"
     CDS             complement(19421..20503)
                     /gene="prfA"
                     /locus_tag="KLK84_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBE2008896.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(20545..21801)
                     /gene="hemA"
                     /locus_tag="KLK84_001523"
     CDS             complement(20545..21801)
                     /gene="hemA"
                     /locus_tag="KLK84_001523"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HBE2008897.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            22015..22638
                     /gene="lolB"
                     /locus_tag="KLK84_001524"
     CDS             22015..22638
                     /gene="lolB"
                     /locus_tag="KLK84_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HBE2008898.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            22638..23489
                     /gene="ispE"
                     /locus_tag="KLK84_001525"
     CDS             22638..23489
                     /gene="ispE"
                     /locus_tag="KLK84_001525"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBE2008899.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            23640..24587
                     /gene="prs"
                     /locus_tag="KLK84_001526"
     CDS             23640..24587
                     /gene="prs"
                     /locus_tag="KLK84_001526"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBE2008900.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            24712..26391
                     /gene="dauA"
                     /locus_tag="KLK84_001527"
     CDS             24712..26391
                     /gene="dauA"
                     /locus_tag="KLK84_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HBE2008901.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(26446..26724)
                     /gene="ychH"
                     /locus_tag="KLK84_001528"
     CDS             complement(26446..26724)
                     /gene="ychH"
                     /locus_tag="KLK84_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HBE2008902.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            27002..27586
                     /gene="pth"
                     /locus_tag="KLK84_001529"
     CDS             27002..27586
                     /gene="pth"
                     /locus_tag="KLK84_001529"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBE2008903.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            27703..28794
                     /gene="ychF"
                     /locus_tag="KLK84_001530"
     CDS             27703..28794
                     /gene="ychF"
                     /locus_tag="KLK84_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBE2008904.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            29637..32504
                     /locus_tag="KLK84_001531"
     CDS             29637..32504
                     /locus_tag="KLK84_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBE2008905.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSPDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(32604..34523)
                     /gene="dhaR"
                     /locus_tag="KLK84_001532"
     CDS             complement(32604..34523)
                     /gene="dhaR"
                     /locus_tag="KLK84_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HBE2008906.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTVDLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            34751..35821
                     /gene="dhaK"
                     /locus_tag="KLK84_001533"
     CDS             34751..35821
                     /gene="dhaK"
                     /locus_tag="KLK84_001533"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBE2008907.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLEACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            35832..36464
                     /gene="dhaL"
                     /locus_tag="KLK84_001534"
     CDS             35832..36464
                     /gene="dhaL"
                     /locus_tag="KLK84_001534"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HBE2008908.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            36475..37893
                     /gene="dhaM"
                     /locus_tag="KLK84_001535"
     CDS             36475..37893
                     /gene="dhaM"
                     /locus_tag="KLK84_001535"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HBE2008909.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            38213..39910
                     /gene="treA"
                     /locus_tag="KLK84_001536"
     CDS             38213..39910
                     /gene="treA"
                     /locus_tag="KLK84_001536"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HBE2008910.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(39989..40428)
                     /locus_tag="KLK84_001537"
                     /pseudo
     CDS             complement(39989..40428)
                     /locus_tag="KLK84_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        10087 a        10577 c        10175 g         9964 t
ORIGIN      
        1 cttccttgaa taatcaaaac ctgttactcc gagctaattt tgcccgatgg caccacgttt
       61 accgggccta caatatcctc ccctgtaggt cggataagcg aagcgcatcc gacaactcac
      121 cccgcaccgt gcccataaaa aaaccccgcc gaagcagggt ctatgtgaac taaaattcgt
      181 ttagtgacga gcgcgcacca gctggtactt acgtgtcaga tactccaccg gtacgctcca
      241 gatgtgtacc agacgcgaga acgggaacag caggaataac gtcatcccca gcaccaggtg
      301 cagacggaag ataaacgcca caccatcgag gtgttgagaa gcgccaccgt ggaaggtcac
      361 caccgactgc gcccagccaa ccagtttcat catctcgcta ccgtccatat gctgagcgga
      421 gaacggaatg gtcagcaggc ccagcgcgca ctggataacg agcagcgaca ggatcaggat
      481 atccgctccg gtagtggttg cacgcacgcg tgggctgaac atacgacgtt tcagcaaaag
      541 cacgccgcca atcagacaca gcacgccgct ggcaccacca gcaaacatcg ccattttctg
      601 tttcacttca atcggcagcc aggcttcata catccagtgc ggcgtcaaca taccgaagaa
      661 gtgaccgaca aaaatcccca gaataccgat atggaacagg ttcgacgcca ggttcatccc
      721 tttgcgatcc agcatctggc tggacgccgc gcgccaggtg tactgcccgt agtcataacg
      781 cagccagcta ccaatcagga agaccgcccc ggctatgtac gggtagatat caaagaagaa
      841 catattcagg aattgcatta gtgctgtcct ccggtggtga tattcagata ttgcggcgcg
      901 acggcaccgg caaagcgacg ctgatgagca gtgattgctg aatcaccgca gcctttgtca
      961 gcaaagaatt taacctgctc ttcttcccag acagcatcca gcgcctgcgg cgtatcatcg
     1021 cgcgcttcgt cggcaatttt ttccgccact ttgtcgctgt cgatagcggt attggccaat
     1081 ttcagcagca gatcaaacat cacggcataa cggctttcac gctgttgcag acgcgcgctc
     1141 agcaatgcca gaatcggcgc gatatctttc aaaccttcca cggcttcgct ttgcggcagc
     1201 tgcgacaggt actccagata cagcggcaga tggtccggta attcgcggct gtttaactgc
     1261 aagccgtgct gctcgtactg cgccagcagg tccaccatcg cctggccgcg gtcacgggat
     1321 tcaccgtgaa catgttcaaa tagcaacagt gacgtggcgc ggccacggtc gaacagttcg
     1381 ctgtactgga cctgggcatc gagcggatcc atcgccgtta aatcgcgcag gaaaatgccc
     1441 agcgcatggg catcttcttt cgacagattt ttcgacgcag caatcgcctc aaacatctct
     1501 tgttgatgct gccataaggc agcatccgga tattcaagga gacgcgatac aatcacgagt
     1561 tcgatcatgg atgcggctcc gttttgctgg tcacatcgat ggcatcgata cgacggctgt
     1621 tgaacagatt gaatttggta tctgagccgt ggcagccatc accaaaggta aagccgcagc
     1681 catttttctc cgggaaggct tcccgtgcca gttcacgatg actactcggc accacaaagc
     1741 gatcttcgta gttagcaatc gccagataac ggtacatctc ctgtgcctgg gcttcggtca
     1801 gaccgacctc ttccagcgca cgggtatcaa ctttaccgtc aacagtttca gcacgtttgt
     1861 aatgacgcat cgccagcata cgtttcagcg cacgcagtac cggtttggta tcaccggcgg
     1921 tcagcagatt cgccagatac tgtaccggaa tacgcaagct ttcgacgtct ggcagaattc
     1981 cgttgctgcc cagctcgccc gcgtctgctg cagactgaat cggagacaga ggcggcacgt
     2041 accagaccat cggcagtgtg cgatattctg gatgcagcgg cagcgctagt ttccattcca
     2101 tcgccatttt ataaaccggc gactgctgtg cagcttcaat aacgctcagc ggaataccgt
     2161 ctttaatcgc ctgctcgatg actttcggat cgttcggatc gaggaacacg tccagctgac
     2221 gctggtagag atctttctcg ttctcggtgc tggctgcacg ttcaatcgcg tcggcatcgt
     2281 acagcagcac gccaagataa cggatacgac cgacacaggt ttctgagcac acggtcggct
     2341 gacccgcttc aatacgcgga tagcagaaga tgcacttctc agacttaccg ctcttccagt
     2401 tgaagtagat ttttttgtac gggcatccgg tgatgcacat acgccagcca cggcatttat
     2461 cctggtcgat caggacgatg ccatcttctt cacgcttgta aatcgcaccg ctcgggcagg
     2521 tcgccacaca tgccgggttc aggcagtgtt cgcacaggcg cggcaaatac atcataaagg
     2581 tgttttcgaa ctggctatac atcgccttct ggatgttgtc gaagttcttg tctttcgcca
     2641 gtttatcaaa ctcaccaccc aggtcatctt cccagttcgg ccctttttcg attttcgcca
     2701 tccgttcccc ggtaatcagc gaacgcggac gggcaatcgg ctgcgatttg ctgccttccg
     2761 gcgcggtatg caggttctga taatcaaaat cgaacggctc gtaataatcg tcgatccccg
     2821 gcagatgcgg gttagcaaag attttaccca gcagcatggc tcggttaccc atgcgcggtt
     2881 gcagtttgcc attgatttta cggatccagc cgcctttgta tttttcctgg ttttcccagt
     2941 cagtcgggaa gccctggccc ggcttggttt ccacgttgtt gaaccacgcg tattccacgc
     3001 cttcacggct ggtccagacg tttttacagg taactgaaca ggtgtggcag ccgatgcact
     3061 tatcgagatt cagcaccatg ccgacttgtg aacgaatttt cattttacgc tctcctgtac
     3121 ctggtcattg ccttcgccat ctaaccagtc aatgttcttc atcttacgca ctacaacaaa
     3181 ctcatcgcgg ttagaaccta cggtgccgta gtagttaaag ccgtatgcca gatgggcata
     3241 gccgccgatc atatgcgtcg gtttcggcgt gatacgggtg accgagttat ggataccacc
     3301 acgctgttgg gtaatttccg aaccaggcag gttaacgata cgttcctgcg cgtggtacat
     3361 catggtcatc cctgccggaa cacgctggct gacaaccgca cgggcagtca gtgcaccgtt
     3421 gctgttgaag acttcaatcc agtcgttatc ggcgataccc agatctttgg catcggcttc
     3481 actcaaccag accaccggac caccgcgacc taaagtcagc atcagcaggt tgtcgctgta
     3541 ggtggagtgg ataccccact tctggtgcgg cgtcaggaag ttgagcgctt tttccgggtt
     3601 gccgttggat ttctggccta tcacttcttt caccgaacgg gtgtcgatcg gcggacgata
     3661 aaccagcagg ctttcaccga aatcacgcat ccactggtga tcctgataca gttgctgacg
     3721 accagagagc gtacgccatg ggatcagctc gtgaacgttg gtgtaaccgg cgttgtaaga
     3781 aacgtgttca tcttccagac cagaccaagt cgggctggag ataattttgc gcggctgtgc
     3841 ctgaatatcg cggaagcgga tcttctcgtc ttctttattc agcgccagat gcgtatggtc
     3901 acgaccggta aattcgctca gggcagccca ggctttcacg gctacctgac cgttggtttc
     3961 cggtgccagc gtcaggatca tctctgccgc atcaattgcg gtgttcagca tcggctggcc
     4021 ttttgccgga ccttccgctt tggtgtagtt gagcttacgc agcagatcca tctcgctctg
     4081 ggtgttccaa gcaatccctt taccgccgtt accgattttc tccatcagcg ggccgataga
     4141 ggtaaagcgt tcgtaagttg ccggataatc gcgctctacg accataatgt gcggtgcagt
     4201 tttgcctggg atcaggtcgc attcgccttt tttccagtct ttcacatcca gcggctgcgc
     4261 cagttcagcg gcagagtcgt gctggatagg cagcgtgacg atgtcggttt ctttacccag
     4321 atagccgacg cacacttcgg agaatttctt cgcgatggct ttgtagattt cccagtcgct
     4381 tttcgcttcc caggccggat cgaccgccgc agacagcggg tgaataaacg gatgcatatc
     4441 cgaagtattc atgtcgtctt tttcgtacca ggtcgccgtc ggcaaaatga tgtcggaata
     4501 gagacaggtg ctcgacagac ggaagtccag cgtaaccacc agatccagct tgccttccag
     4561 accattgtcc tgccagtcca cttcttccgg cttcacgccg ccctgttgcc ccagatcttt
     4621 accctggata ccgtgctccg tccccagcag gtacttgagc ataaactcat gacctttacc
     4681 ggaagaaccg agcaggttag aacgccagat gaacaggttg cgcgggtggt ttttaccgtt
     4741 ttctggttgt tctgccgcaa aacggatgga accctctttc agggatttca ccgtatagtc
     4801 caccggattc atcccggctt tttccgcttc gccagcgata gtcagcgggt tagtgcctaa
     4861 ctgcggtgca gacggcagcc agcccatgcg ttccgcacgg acgttaaagt cgatcaagtg
     4921 tccggtatag cgggatttgt ccgccatcgg tgacagcaac tcttccgccg tgacggtttc
     4981 ataacgccac tggctggagt ggttatagaa ataagaagtg ctgttcatgt gacgcgccgg
     5041 acgctgccag tcaagggcaa acgccagcgg ctgccagccg gtttgcggac gcagtttttc
     5101 ctgacctaca tagtgcgccc agccgccccc gctctgaccg acacagccgc agaaaatcag
     5161 catgttgatc agaccacgat agttcatatc gaggtgatac cagtggttca gccccgcacc
     5221 gacgataatc atcgaacgac cgtgcgtttt atcagcgtta tcggcaaatt cacgggcgat
     5281 gcgaataatc tggctgcgag aaacgccggt aatctgctcg gcccaagccg gggtataagc
     5341 tttcacatcg tcatagctgg ttgcacagtt aacgtcgttc aggccacgtt ccagaccgta
     5401 gtttgccagc gtcagatcat aaacggtggt caccagggcg gtgctgccat cagccagttg
     5461 caggcgtttc accggcagtt tgtgcagcag cacgttttcc agttccactt tgttgaagtg
     5521 ttccgtgccg tcgccaccaa agtacgggaa gcctacctca gcgatctcat cctggctacc
     5581 cagcaggctc agttgcagct cggtttcttc gccagttttg ccgtcgcgct gctcaagatt
     5641 ccatttgccc ttctcgcccc agcggaagcc aatagaaccg ttcggcgcaa ccatttcgcc
     5701 attggtatta aaggcgacag ttttccattc cggattgttt tcctggccca gcgcatcaac
     5761 cagatcagca gcgcgcagca tacgacctgc agcgtagtaa ccgtcgcgtt cttccagcat
     5821 caccagcatc ggcatgtcgg tgtagcgacg cacatagtcg gtgaaatact ggcttgggtt
     5881 gtcgaggtgg aattcacgca gcattacgtg gcccatcgcc agcgccattg ccgcatcggt
     5941 gccctgtttc ggtgccagcc acagatcgca cagtttggcg atttcagcgt agtctggtgt
     6001 gacggcaaca gttttggtgc ctttgtaacg cacttcagta aagaagtgag catccggggt
     6061 acgcgtctgc ggcacgtttg aaccccaggc gatgatgtag ctggagttat accagtcagc
     6121 agattccggt acgtcagttt gctcgcccca ggtttgcgga gacgcaggag gcaggtcgca
     6181 gtaccagtcg tagaagctta agcaagtacc gccaatcagc gagagatagc gtgcacccga
     6241 tgcgtaagaa accatcgaca ttgccggaat tggcgagaaa ccagcaacac ggtccgggcc
     6301 gtagtttttg atggtgtaaa cgttagatgc ggcgatcagt tcgttcacct cctgccaaga
     6361 agaacgaaca aatccaccgc gtccacgcgc ctgcttaaag cttttcgctt tatcggcgtc
     6421 ttcaatgata gaagcccatg cctcaaccgg atcgctatgc agcgccttcg cttcacgcca
     6481 cattttcatc aggcgtttgc gcatcatcgg gtatttcagg cggttggcac tgtaaagata
     6541 ccaggagtag ctggcaccgc gcgggcagcc gcgaggttca tggtttggca gatccggacg
     6601 ggtacgcgga tagtcagtct gctgggtttc ccaggtgacc agaccgtttt tgacgtagat
     6661 tttccagctg caggagccgg tgcagtttac cccgtgggta gagcggacga ttttgtcatg
     6721 ctgccaacgc tggcgatatc catcctccca gtcacggttg gtattgagaa gctggccatg
     6781 cccatcggca aaggtttcac ccttctgttt gaagtagcga aaccggtcca ggaatttact
     6841 catcggtttt ctcctgtggg agcctgtcgg ccctctgata aatcgacatt gctaatgttt
     6901 aagggcaagg taacgctctg aaacagcgtg ggaattgata acgatcaaga gtgatgggga
     6961 tgaaaaataa agtaaattcc taagataccc ctttaggata attaacagtt acagcataac
     7021 atgctgtaaa ttaagacgtt ttatacctgt agttatttaa actattaaga tttttatgca
     7081 aatgggtatt aaggagtatt ccccatgggt aacatattaa tggctcttaa tgaaatccgg
     7141 cgaaataagc catataacac taatatgttg taactaaatg acgcataaaa aaagcgcggc
     7201 atcatgccgc gcaaaggata atcaaatatt aagatttatt ttttagaatg ccgaccatat
     7261 accgcccaag taatcactac gcaggcgata tagaaaatca aaaatacttt cattgcacct
     7321 actggcgaac ccgttaatgc cagcgagcta ccaaacgctt tcgggataaa gaagccacca
     7381 atcgcgccaa tcgcagagat gaaacccagc gccgctgccg tgccggttgc cgcttcacgc
     7441 atcgcacgtt cgtcagaacc cccttctgct ttcacgcgat ccattgtcag tttacggaag
     7501 atcactgaaa tcatctggaa agtggaacca ctacccagcc cagctgtcag gaacagcgcc
     7561 aggaagaccg cgaagaacgc catgaagctt ccgccctgcc cgtcagtcgg taaggtcagg
     7621 aacagcaggc cgctgaaaat cgccatcaga ataaagttca ccagcgtgac acgagttccg
     7681 cccagacggt cagataatgc accacctgca gaacgcgcca gcgcaccaat aaacggcccg
     7741 aagaaagcgt attgcagaat ctgaacatcc gggaactgcg tttttgacag catcgcaaag
     7801 cccgcggaga agccgatgaa ggagccgaag gttgccagat acagcaggct cataatccac
     7861 agatgacccc gtttgagtac cggcaactgc tccttgatgg aggctttcga ggtagcaaga
     7921 tcgttcatgc caaaccacgc cgcaatggtg aagatggcaa ggaacggcac ccatatccag
     7981 gacgcattcg ccagatacag ctcagtccca tccggctgtt tgacgccctg gctaccaaat
     8041 actgcgaaaa tcgacagtga taccaccagc ggagcaacca actgcatgac gctgacgccc
     8101 atgtttccca gaccaccatt cagacccagc gcgccaccct gcttctgttt cggaaagaag
     8161 aagctgatgt ttgccatact ggatgcgaag ttcgcgccag caaagccgca tagcagagag
     8221 atgatgatga agacgctata aggcgtggag gtatcctgca cggcaaaacc cagccagacg
     8281 caaggaataa tcagaatacc ggtgctgaac gccgtccagc gacgaccacc gaagatagga
     8341 accataaagg agtatggaac acgtaataac gcgccagaaa ccgaaggcag cgcagtcaac
     8401 ataaatagct gatcggtcgt aaaattaaaa ccgactttcg gtaagttcac cgcaacagcg
     8461 ctgaacaaca tccatacgca aaacgccagc agcagacagg gaacggaaat ccacaggttg
     8521 cggctggcaa tacgttgacc gcgttgttgc cagaacgcag gatcttccgg tcgccaatct
     8581 gttatgacag ctccagtagc cctttcggag gcagatgagt gactcataga cacctctgat
     8641 actcgtttcg atgtctgcca ccttagtgtc tggagctaaa ggcaatttga tgtaaatcaa
     8701 acgataagat aactttatct atcattgata tttatcatta cccatagtga gtacagtgat
     8761 ttcataaaaa ttatgagatt tttcacggtg ctgtagaatc cctaccctta ccgatgtaaa
     8821 gcgactaacc acacggcaaa taaggagtaa ctctttccgg ctatgggtat acttcagcca
     8881 atagccgaga atactgccat tccagaatat atcgtcacat tcattaaggt tattgctcat
     8941 ttaaagcctg aaggaagggg tttacatgct taaacgttgt ctctctccgc tcaccctggt
     9001 taatcaggtt gcgcttattg tgttgctttc tactgctatt ggactggcag ggatggcggt
     9061 ttctggctgg ctggtgcaag gcgttcaggg cagcgcccat gcgatcaaca aagcgggatc
     9121 gctgcgcatg caaagttacc gtctgttggc ggcagtgcca ttaagcgaga aagacaagcc
     9181 cttaattaaa gagatggaac aaacggcatt tagcgccgag ttgactcgag cagcagaacg
     9241 agacggacaa ctggcgcaat tacagggttt acaagattac tggcgtaatg aactgatccc
     9301 tgcgctgatg cgtgcacaaa accgagaaac ggtgtcagcg gatgtcagcc agtttgttgc
     9361 cgggcttgat cagctggtat ctggttttga ccgcaccacg gaaatgcgta ttgagacagt
     9421 ggtactggtc catcgggtaa tggcggtatt tatggcactt ttactggtgt tcactattat
     9481 ctggttgcgg gcgcgactgc tacaaccgtg gcggcaactg ctggcaatgg cgagtgccgt
     9541 cagccatcgc gattttaccc aacgcgcaaa catcagcggg cgcaacgaaa tggcgatgct
     9601 tggaactgcg ttgaacaata tgtctgcaga actggccgaa agttatgccg tacttgagca
     9661 gcgggttcag gagaaaaccg ccgggctgga gcataaaaat cagatcctct cttttttatg
     9721 gcaggctaac cgccgtttgc attcccgcgc cccgctgtgt gaacgcctgt caccggtact
     9781 caacggctta cagaatttaa ccctgctacg tgatatcgaa ctgcgggtgt atgacactga
     9841 tgatgaagag aatcatcagg agtttacctg ccagccagat atgacttgtg atgataaagg
     9901 ctgccagctc tgcccgcgcg gcgtattacc cgttggtgat cgcggcacga ccctgaagtg
     9961 gcggctggct gactctcata cgcagtacgg tattttgctg gcgaccctgc cgcaggggcg
    10021 tcatcttagc catgatcaac aacaactggt ggataccctg gttgaacaac ttaccgccac
    10081 gctggcgctg gatcgccatc aggaacgtca gcaacagttg atcgtgatgg aagagcgtgc
    10141 caccattgcg cgcgaactgc atgattctat tgcccaatct ctctcttgca tgaagatgca
    10201 ggtgagttgt ttacagatgc agggcgatgc gctgccagaa agcagccgcg aactgttaag
    10261 tcagatccgt aacgaactga atgcatcctg ggcgcagttg cgtgaattgc tcaccacatt
    10321 ccgcttgcag ctcaccgagc ctggattacg tccggcgctg gaagcgagtt gcgaagagta
    10381 cagcgccaaa tttggcttcc cggtgaagct ggattatcaa ttgccgcctc gtctggtgcc
    10441 ttcacatcag gcaatccact tgttgcaaat tgcccgtgag gcattaagta acgccctcaa
    10501 acattcgcaa gcgagtgagg tcgtggtgac ggtggcgcaa aacgataatc aggtcaaact
    10561 gaccgtccag gataacggct gcggcgtgcc tgaaaatgcc atccgcagca atcactacgg
    10621 catgataata atgcgcgacc gtgcgcaaag tttacgaggc gattgccgcg tccgccgtcg
    10681 tgaatcaggt ggcaccgaag tggtggtcac ctttattccc gaaaaaactt tcacagacgt
    10741 ccaaggagat acccatgagt aatcaggaac cggctactat cctgctgatt gacgatcacc
    10801 cgatgctgcg aactggcgta aaacagctta tcagtatggc accagatatc accgtggttg
    10861 gcgaagcgag taatggcgaa cagggtattg aactggcgga atctcttgat cccgatctga
    10921 tcctgttaga tctcaatatg cccggcatga acggtctgga aacgctggat aaactgcgcg
    10981 aaaagtccct ctcagggcgc attgtggtat tcagcgtctc taaccatgaa gaagatgtgg
    11041 tcaccgcact gaaacgcggc gcggatggct atctgttaaa agatatggaa ccggaagatc
    11101 tgctgaaagc attgcatcag gcagctgctg gcgaaatggt attaagcgaa gcattaacgc
    11161 ctgttctggc cgccagcttg cgcgctaacc gtgccactac tgagcgcgat gttaaccagt
    11221 taaccccacg cgagcgcgat attctcaagc tgattgccca gggtttgccg aacaagatga
    11281 ttgcccgccg cctggatatc accgaaagca cagtaaaagt gcacgtcaag cacatgctga
    11341 agaaaatgaa gctcaagtct cgcgtggaag cagcggtatg ggtgcatcag gagcgcattt
    11401 tctgattacg gttcccagcg cagttcgtcg tttgacaatg catcgaacgg ttcgacaagc
    11461 gttagcgtga tctcattgga ggagacacgc tgcccctggt tatcttccac caccacagac
    11521 aatcgccagt gattgcttgc cccttccccg ttctgccagt caggcatgat cagcgtccag
    11581 ccctccgcgc tgttggcttg tgcgcccggc gtcaaactta atatctgcgt atcgccctgc
    11641 caaatcagtt gccgaatacc gtaacgactg cggatttgta atttcagcgg cactgtttcg
    11701 ccaggtttta gatcccacgg cggtgtcgcc agaaacaccg ttaacgtttt tcgctgtcgg
    11761 tactcaagag tcggtagatt atttcgctgc ggattgtcat agcgactacc acgtaacgac
    11821 tgactttcgg caacctcgcc cgcagaaagt tgttttttga gcggtacacc aaagcggtaa
    11881 ttaagattca gcccgaggtt attttgattt tcgccacttt ccccctgttt atgctgggcc
    11941 gtcacagtga ctaatggcac aggggtgtaa tttaatccca gactcaacgc gacgggattg
    12001 tgataacccg taccagagtt aaacaaatca acacgatcac caaaatactg ttctaggctg
    12061 acactggtat tgaggtgttg atagaacggc atgcgcatcc gagctgtcag gtcgtacccg
    12121 cgcgccatcc gttgttcctg cgtggctgtc tgttcatgcc atgcagcaaa cggctgataa
    12181 aagtttgccg ataatcgcaa atattcgccc cacgcttcgg caccaaagcc cgctcgctga
    12241 agattttcgt cctgcaagtt gtcataaaaa gtgttataac ccaccagcca gttgccgcgc
    12301 gcccagcgtt gcccaacgcc cacattgctc accaacccat tatcctgctg agtaagacca
    12361 agctggctcc aggtgagata acgatcatta tcttgtaacg gcacaaacca gcttccacga
    12421 ctgccggtga aatgtccttc gttatccact ttgacgtcaa cactggcatt tccccacggt
    12481 gatagccagg actctacgtg ctgattaacc tgttgactaa gcgcgtcgcg gacttttccc
    12541 aatgcgaaag cttttgcctg ctcgccagta tccagcccgt tatcattcat actggtttcg
    12601 ccgaaatctt tcacaatttc agcaaagtgt ttttccccat catgattttc gggagccatg
    12661 cctaaatccg gcagaccatc attgttatta tcaaagggat ttgccgcttt ttgctcgaag
    12721 gtagattgtg cgttagctgt accgcctgct acaagcaaga gtaaataaaa cgggataatg
    12781 cgaggaacga aacggctcaa aacgtcggta acttccggta ataatgaaaa accatagcct
    12841 taaagaataa cgattactgg caacttttgt cgtattttct cgcgctttca ggaaaatcct
    12901 taaaaatgtg accgattggt cagacgctgc agaagcagta agatagaaga tgagtgtcta
    12961 attccctgaa tacaggagta aataatgcaa aaaatcgtga tcgttgccaa tggcgcacct
    13021 tacgggagcg aatccttgtt taacagcttg cggctggcca ttgcgttacg agagcaggag
    13081 agcaatctgg atctgcgtct gttcctgatg tctgatgcgg tcacagccgg gttgcgcggg
    13141 caaaaaccag gggaaggcta caacattcag caaatgctgg agatccttac cgctcagaat
    13201 gtaccggtga aattgtgcaa aacctgtacc gacgggcgcg ggattagcac acttcctctg
    13261 attgatgggg tggaaatcgg tactcttgtg gaactggcgc aatggacgct gtcagccgat
    13321 aaagtgctca cattttaatg tttaaggggg cgtctgacgc cccactatga gggaagttta
    13381 ctcaggcgaa tcccggcctt aacacaccat ccggataatt ctccgccagt agttttttta
    13441 ccgataccag caaatcattc agcccatcgt cctgcattcc cagtttgata agctcctgtt
    13501 ccagtgaaaa caggtattgt gcattggttc ccagcggacc gctcgccgcc gcaatcaacg
    13561 gcgcgatgac ctgagcgcga gtgtcagatt catattctgg atgtcgcggg tccataataa
    13621 acacaatggc gtttactgtg cgtccatcat caagatcaag ctgacaccag gttggcagat
    13681 aacagccggt aatcatctcg cgcttccaca acagggttag ttcctgctcc agcgtctctt
    13741 ctggtagtcg ataggcgacg cctgtggtgc gtccgccctc tttcagtgca agcatccgtc
    13801 ccggctggtg cgcagtcccg cgcccggcgg tcaggcgcag gcaaaacgcg cgatgccatc
    13861 caaccagtgt accggtgcat gattcggtaa actccagtgc cggattccac atcaacgatc
    13921 cataaccgaa gatccacacc ggtccctcat caggtcgaca agccagcgtc gccgccagcg
    13981 aagccgcccg ttgttctgct gaccataaga gtgattcttc aatggcacca aatgccgttt
    14041 tacaatcggc attcatcaag aaatcacgcg ttatcacctt acacctccgc cactgcatac
    14101 ttccctgcga caactttttg gtgtcttccg gacattttta ccgctcattt tgaaaccact
    14161 aacggaacaa tatgcaatcc acggaccact tgcaaggcaa caccgcacga atagctttaa
    14221 ctaagctacc ggttttacga ttttttatgc catttatcat catccccttt ggcatattca
    14281 tgcttcacag cagcccaggc cactttatgc gcggtttctt cgcgactggc gtcatcacgc
    14341 cgatcttctt tatctttata ttgatcccat gcgctgttga acgcttcttt atagatatcc
    14401 tgggcatgag acggtagaac gtgctttacg ctttccggca gatcgctttt cgttttatac
    14461 ggcatcgttg actcctttta caaagttgaa cattaagtgt ggtcaacaat atctactctt
    14521 gccaaaacat aagaattttc atgtcagact gttcttaaga aacgctaaaa caaacattgt
    14581 ctaaacgtaa tacttatttt tatgccccaa tgagtaaaaa atcgacagcg gggtaaaaat
    14641 tcgtaaaatg ttaccgatat ccagacactt tctgttattt ttcctaccta ttttttacat
    14701 tttcgataaa ttcatctgca aagggataac cataatgtca aatgctcaag aggcggtaaa
    14761 aacccgccac aaggagactt cgcttatttt cccggttctg gtgctggtag tgctgttcct
    14821 gtggggaagt agccagacac taccagtggt cattgccatc aatcttcttg cgcttattgg
    14881 tattttaagt agcgccttta gtgttgtccg tcatgcggac gtattagccc atcgcctggg
    14941 agaaccttac ggttcgctta ttcttagcct ttcagtggtt attcttgaag tcagtttgat
    15001 ttcagcttta atggcaaccg gcgacgccgc gccaacgcta atgcgtgata cgctctattc
    15061 aatcattatg attgttaccg gtgggctggt tggcttttca ttattgttgg gcggtcgtaa
    15121 gtttgccacc caatatatga atctgtttgg tatcaagcag tatttaattg cgctgttccc
    15181 cctggcgata atcgtactgg tatttccaat ggctctgcct gcggcgaatt tttcaaccgg
    15241 tcaggcgtta ctggtagcat taatttctgc ggcaatgtat ggcgtatttt tgctgatcca
    15301 ggccaaaacg catcaaagtt tgtttgtcta cgagcacgaa gatgacagtg atgacgacga
    15361 cccgcaccac ggtaaaacgt ctgcccatag cagcctgtgg catgctatct ggttgattat
    15421 ccatctgatt gccgttattg cagtcaccaa aatgaacgcc agctcgctgg agacattgct
    15481 cgacagcatg aatgcccctg ttgcctttac tggcttcctg gtggcactgt tgattctgtc
    15541 gccggaaggt ttaggtgcgt taaaagcagt gttgaacaac caggttcagc gcgcgatgaa
    15601 tctgttcttt ggttcagtgt tagcaacaat ttcgcttacc gtacctgtcg tcacgctaat
    15661 tgcctttatg acgggtaacg aattgcagtt tgcacttggt gcgccagaaa tggtggtgat
    15721 ggtggcctct ttagtgctgt gccatatctc cttctccacc ggacgtacta acgtgctcaa
    15781 tggcgcagcg catctggcac tgtttgccgc ctatttgatg acgatatttg cctgacagtt
    15841 atatctcctc cgatgacgga aatcgggaga gataagataa attcctgctt attgtatttc
    15901 ttcatactac ctggacagct cgttaatcag aattgcgagc ctgtaccaca acctgcacga
    15961 aaccactgca acagctgttg aaaagccgga atgtactctt tattgcaaac atttcaggga
    16021 tgaagtgaag atgaattgag acgtagtatc cacaccaagc cggaattatc cggtgaggcg
    16081 caatgttgcg ggggctttat ccctggtggc attggttgct ggaaagagaa aacccccgca
    16141 cgttgagagg taaaacctga caacaaaacg ggggctaatc ttgaaaccag acacttcaag
    16201 aatagccgca aaatgttgtc attacaacaa ggcggctata tgacgctcgc gcagtttgcc
    16261 atgactttct ggcacgacct ggcagcaccg atcctggcgg ggattattgc cgcagcgatt
    16321 gtcagctggt ggcgtaaccg gaagtaaggt gaaaacgggc gtcaggctgc cgtgatggca
    16381 atatgcgccc gaaaccggcc gcaggggtaa cagcctgcgg ccttttttgt atctggcacc
    16441 catgaaaaaa gtcttaacgc agaacgctaa gactttattt ttcaagcaaa aaagattact
    16501 tgctggtatc cagttcttcg aaacctttca ccagatcatc aatcgctttc atctgcttga
    16561 ggaacggttc cagtttagcc agcggcagcg cggatggacc atcacatttc gcatgttccg
    16621 gatccggatg cgcttcaata aacagccccg ccagacctac cgccataccg gctcgtgcca
    16681 gctcagccac ctgagcacga cgaccaccgg aagctgcgcc aaacggatcg cggcattgca
    16741 gtgcgtgggt cacgtcgaaa atcaccggcg agttaccaga cactttcttc ataatgctga
    16801 agcccagcat atcgacaacc aggttgtcat agccgaagtt agcaccacga tcgcaaagaa
    16861 tcactttttc gttgccgcct tctttgaatt tatcaacgat attacccatc tgtcccgggc
    16921 tgacaaactg tggtttcttg acgttaatta ccgcaccggt tttcgccatg gcttcaacca
    16981 ggtcagtctg gcgagcaaga aacgccggca actgaatcac atccacgaca tcagcaacgg
    17041 gctgtgcctg acttggttcg tgaacgtcgg tgataatttt cacgccaaaa gtctgcttca
    17101 actcctggaa gattttcatc ccttcttcca ggcccggtcc acgataagag tggatggagg
    17161 agcggttggc tttgtcaaaa gaggctttga acacgtaagg gatacccagt ttctgggtca
    17221 cagttacgta gtgctcgcaa atgcgcatcg ccagatcgcg agattccagc acgttcatac
    17281 cgccaaacag tacgaacggc aggtcatttg ctacgttgat gtcgccaatg ctaaccactt
    17341 tttgtttcat aggatcgcct taatcttgag taaaatgtcg attaattaat gcagcacaat
    17401 atgtttatgc gcgatgttat ttatttgcgc acggatcatt tcgctgatcg ggtcttccgg
    17461 acactgttca acgaaataac ttaaatcgtt caacgcaacg tgttcgcaat ccagttgcgc
    17521 ataaatcaac ccgcgatcgc gaatttcata gggatcttca gggttgaatt gtaataaagc
    17581 ttcgctggtg cgtaacgcca gctccatctg attttcttcc atcaacgagg ctttgagtgt
    17641 atccagcaat ttgcggatta cctcaatgtt atcagcttca tcaaggtctt cataaaacag
    17701 ttccgccgac gggctgatat ttccctttaa ccatccgtcc agcatatgtt cgcttaacga
    17761 ttcaccgtta aaaggattaa tcagccaaat ttcgccatcc ggacattcaa tgcgcaatat
    17821 cagctgcgta gggaaaatca ccggcagcag cggcaaatcg agacgattcg cgacccataa
    17881 taaaaccgca cctaatgata ccgcactgcc ctgtcgattc tttaacacct ggtccagcca
    17941 taatgcatcg gaaagacgat aaacgccgcg tgaggcttta aatccccagt cgccgtaaaa
    18001 cagcgcgatc agtttttcca gctgctcttc taaaggcaga agctggctga tttcttcctt
    18061 cgccagacta acgagacgct ccagttcgtc gtaaacatct tgcgagggaa aatcgcggcg
    18121 gattgcttcg caagccagga tcatgccttc gcacaatggc gctttattaa attcgaaatc
    18181 agctaacgat ctcatgactt accccagtaa cggtactttt gtggtggcga gtttaatgat
    18241 gatgtacagc accaccaacg ccagcgggaa ggcaataatg cgcgcctgct gactgtgcat
    18301 acgacgataa tcgagtgcaa taaaacccaa aacgatataa atgataactc caaacagctt
    18361 ttcagtcagc cactgtgcct gcccggaaaa tggcaggatg tgcgctttag ccatcaacgc
    18421 aatgccgctt aacaataaca ccgtatcgac aaccggcggc acaatgcgcg tccagcgagc
    18481 aaatgcctgt ggatgcttct gataacgtag ccagaaacgt aaggttaata gcccaacaga
    18541 aagcgcgata ctaataagat gaacactaag cagtgtagaa aaacttgtca ttgataatag
    18601 cggccgagcg ttacgcgctc gttatcacca tagtcacggc aggtttcgac gtcatgatac
    18661 cccgcgagga taaatgcttg tcgcaccgct tcgccctgct gccagccatg ttccagaagc
    18721 agaaagccgc cggataccag cgcgttacgc gactgttcga tgatatgcac gatgtctgcc
    18781 attccactgt ctgccgcaac cagcgcagtg agcggctcaa agcggacatc gccttgttga
    18841 agatgtgggt cctgctcgtc aatatacggc ggattgctga caatcatcgc aaactgctgc
    18901 ccggctagcg cgctaaacca gtcgctttgc agaatgtgga tatttttgat cgccagatgc
    18961 tgggcattac gttgtgccag ggagacagca tcaggcatac gatctacagc gataatttcg
    19021 cagtccgggc gctcgctagc cagcgcaagc gcaatcgccc cggtacccgt cccgagatcg
    19081 agaatacggc aaggttgttc aggcaaccgc gccagtgcct gctccaccag acactccgta
    19141 tccgggcgcg gaattaaggt cgctggcgaa acaaataacg gcaacgacca gaattctcgc
    19201 accccggtta aatgagcaat gggttcacca tcgcgacgac gtgtcagtag cgcatcaagt
    19261 tgctgacatt gttcgtcagt cagctgcgtt tcaccaaagg cgagaataaa agtacgccct
    19321 ctgccggtaa catgctccag caggatttca gcatcacgcc gcgggctttc gctcgcctga
    19381 agttggctta ttgcttcacg taaccagtgt tgatattcca ttattcctgc tcggacaacg
    19441 ccgccagttg gtcggcctga tgttcctgga taatcggttc aatcagcata tccagcttac
    19501 cttccatcac ttcatccagg cggtagagcg tcaggttgat gcggtgatcg gtaacgcgcc
    19561 cctgcgggaa attgtaagta cggttacggt cgctgcgatc gccactcccc agcaggttac
    19621 gacgggtaga cgcttcggcc tgttggcgtt ttgccatttc agcagcgtgg atgcgagcac
    19681 cgagcactga aagcgccttc gctttgtttt tatgttgtga acgttcgtcc tgacattcaa
    19741 caacaatccc ggtcggcaag tgagtaatac gaattgccga atcggtggtg ttaacgtgct
    19801 gaccacccgc ccctgacgag cggaaagtat caatgcgtaa atctgctggg ttgatgtccg
    19861 gcagttctgc gtcaggcagt tctggcatta ccgcaacggt acaagcagaa gtatgaatac
    19921 gaccctgcga ttccgtagca ggaacacgtt gcacgcgatg accgccggat tcaaatttca
    19981 gacgaccata cacaccatca ccgctaattt tggcgatgat ctctttataa ccaccatgtt
    20041 caccctcgct ggcgctcatg atttctaccc gccagcggcg ggcttcggcg taacggctgt
    20101 acatacggaa cagatcgcct gcaaacagcg ctgcttcgtc gccgccggtt ccggcacgga
    20161 cttcgaggaa ggcgttacgt tcgtcatcag gatcttttgg tagtaataga acctgtaatt
    20221 gctgttccag ttgctcgctt ttttctttag cttcgcgcag ttcatcctgc gccatctcac
    20281 gcatttcagg atcgtcgagc atcatctgtg cggtttcgat atcttcctga acctgttgcc
    20341 agtcggtaaa acagcgcgaa acatcactta actgcgcata ttcgcgtgat aatgcgcgaa
    20401 aacgttcctg gtcggcgata gtttgcgcgt cacccagcaa cgcctgaact tcttcatggc
    20461 gttcatgcag ggcttccagt ttggcaacga tagaaggctt cataggcgta aatgcaccct
    20521 gtaaaaaaag aaaatgatgt actgctactc cagcccgagg ctgtcgcgca gaatattcag
    20581 gcgttcgtta tccccgtcac gggcggcctg ttgaagtgat ttcgttggcg catggatcaa
    20641 gcggttagtc agtttccatg ccagatcctg cataatggct tgcgcgtcgc cgccctgctc
    20701 aagggccgct aacgctttgg cggttaactc atcgcgaacg tgctctgcct ggctgcgata
    20761 ctcgcgaatg gtttcgctgg cgctttgtgc tcgcagccac gccataaatt cgctggtttc
    20821 ctgagcgaca atagtttccg cctcaaccgc tgcggcttta cgctgcgcca ggttgtgcga
    20881 aatgatgctt tgcagatcgt ccacactata aagataagca ttcgccagtt tgccaacttc
    20941 cggctcaaca tcgcgcggaa cggcaatatc caccaacagc attggttggt tacgacgact
    21001 ttttaatgcg cgctccacca tgcctttccc gataatcggt aacgggctgg cggtggaact
    21061 gatgatgata tcggcttcgc gcagacgttc gtcgatctca ctcagggcaa tcacttccgc
    21121 gccaacttca tctgccagaa tttgggcacg ttcgcgagtg cggttggcga taatcatctt
    21181 ctgtacttta tgttcgcgca gatgacgcgc taccagctcg atggtttcgc ccgcgcctac
    21241 cagcaacact gtgaccgtag agagcgattc aaagatctgc cgcgccagcg tacaagccgc
    21301 aaaagcgaca gacacagcac tggcaccgat atctgtttca gtgcgaacgc gtttcgctac
    21361 agagaaagat ttctggaaca tgcgttccag ttcgctggcc ttcatatgac ctttttgcga
    21421 atcgacaaac gcttttttaa cctgaccgag gatctgcggc tccccaagaa ccagtgaatc
    21481 caggccgctg gcaacacgca ttaaatggct aaccgcgtcg ttatcctgat gccagtagag
    21541 gcttttacgc agatcttctt cattaagatt gtgataatcg caaagccagc ggattaacgc
    21601 ctcttgcagg ttatcctgct cttcaacgct aagataaagt tccgtgcggt tgcacgtcga
    21661 cagcaccacg ccgccctgca ccatcggctg cgcaagcagg ctgtcaagcg cctgatcgag
    21721 cttatccggc gaaaacgata cacgttctcg cagcgataca ggtgccgttt tatggttgat
    21781 accgagtgct aaaagggtca tgtctgcggg aaataatacc aacgttgata gggttagtct
    21841 gcttgcatca tacaggatgc gtaagatcaa taaaagagag cgcccccttt tggagtaatt
    21901 gccgaaagcc gttagattct ggcaattaag acaacttgaa catagacgat agcggacggt
    21961 aacgctagca ttaagggtta taactgcaac gtatctcaag gacttgtcat cactatgccc
    22021 ctgcccgatt ttcgtcttat ccgcctgcta ccgctggctg ctcttgtgct cactgcctgt
    22081 tccgttacca cgcccaaagg tcctggcaaa agcccggatt cgccacaatg gcgtcagcat
    22141 cagcaagacg tgcgcaatct taatcagtat cagactcgcg gcgcgttcgc ttatatttct
    22201 gaccaacaaa aagtgtacgc ccgctttttc tggcagcaaa ccggccagga tcgctaccgt
    22261 ctgctgctca ctaacccatt gggcagcacg gaactggagc tgaatgctca accgggtaac
    22321 gtgcagttag tcgacaataa aggtcagcgt tataccgccg atgacgccga agagatgatt
    22381 ggcaaattga ccggaatgcc aattccgctc aacagcttgc gccagtggat tttaggttta
    22441 ccgggtgatg caaccgacta caaactggac gaccagtacc gcctgagcga aattacctac
    22501 agccagaatg gcaaaaactg gaaggttgtt tatggtggtt atgacaccaa aacgcaacct
    22561 gcgatgccag ccaatatgga actcaccgac ggtggtcaac gcatcaagtt aaaaatggat
    22621 aactggatag tgaaataatg cggacacagt ggccctctcc ggcaaaactt aatctgtttt
    22681 tatacattac cggtcagcgt gcggatggtt accacacgct gcaaacgctg tttcagtttc
    22741 ttgattacgg cgacaccatc agcattgagc ttcgtgacga tggggatatt cgtctgttaa
    22801 cgcccgttga aggcgtggaa catgaagata acctgatcgt tcgcgcagcg cggttgttga
    22861 tgaaaactgc ggcagacagc gggcgtcttc cgacgggaag cggtgcgaat atcagcattg
    22921 acaagcgttt gccgatgggc ggcggtctcg gcggtggttc atccaatgcc gcgacggtcc
    22981 tggtggcatt aaatcatctc tggcaatgcg ggctaagcat ggatgagctg gcggaaatgg
    23041 ggctgacgct gggcgcagat gttcctgtct ttgttcgggg gcatgccgcg tttgccgaag
    23101 gcgttggtga aatactaacg ccggtggatc cgccagagaa gtggtatctg gtggcgcacc
    23161 ctggtgtaag tattccgact ccggtgattt ttaaagatcc tgaactcccg cgcaatacgc
    23221 caaaaaggtc aatagaaacg ttgctaaaat gtgaattcag caatgattgc gaggttatcg
    23281 caagaaaacg ttttcgcgag gttgatgcgg tgctttcctg gctgttagaa tacgccccgt
    23341 cacgcctgac tgggacaggg gcctgtgtct ttgctgaatt tgatacagag tctgaagccc
    23401 gccaggtgct agagcaagcc ccggaatggc tcaatggctt tgtggcgaaa ggcgttaatc
    23461 tttccccatt gcacagagcc atgctttaag ccgggcaagc tgagtttcgg tgacaacgtc
    23521 accttgttcc agacgttgca tcgcgctctt taatacaccg cctggaaagg atcatgcctg
    23581 gcccgcacag ttttcggcag attctttcca ccaatggacg catgcctgag gttcttctcg
    23641 tgcctgatat gaagcttttt gctggtaacg ccaccccggt actagcacaa cgtattgcca
    23701 accgcctgta cacttcactc ggcgacgccg ctgtaggtcg ctttagcgac ggcgaagtca
    23761 gcgtacaaat taacgaaaat gtacgcggtg gtgatatttt catcatccag tccacttgtg
    23821 cccctactaa cgacaacctg atggaattag tcgttatggt tgatgccctg cgtcgtgctt
    23881 ccgcaggtcg tatcaccgct gttatcccct actttggcta tgcgcgccag gaccgtcgcg
    23941 tccgttccgc tcgtgtacca atcactgcga aagtggttgc agacttcctc tccagcgtcg
    24001 gtgttgaccg tgtgctgaca gtggatctgc acgctgaaca gattcagggt ttcttcgacg
    24061 ttccggttga taacgtattt ggtagcccga tcctgctgga agacatgctg cagctgaatc
    24121 tggataaccc aattgtggtt tctccggaca tcggcggcgt tgtgcgtgcc cgcgctatcg
    24181 ctaagctgct gaacgatacc gatatggcaa tcatcgacaa acgtcgtccg cgtgcgaacg
    24241 tttcccaggt gatgcatatc atcggtgacg ttgcaggtcg tgactgcgta ctggtcgatg
    24301 atatgatcga cactggcggt acgctgtgta aagctgctga agcgctgaaa gaacgtggtg
    24361 ctaaacgtgt atttgcgtac gcgactcacc cgatcttctc tggcaacgcg gcgaacaacc
    24421 tgcgtaactc tgtaattgat gaagtcgttg tctgcgatac cattccgctg agcgatgaaa
    24481 tcaaatcact gccgaacgtg cgtactctga ccctgtcagg tatgctggcc gaagcgattc
    24541 gtcgtatcag caacgaagaa tcgatctctg ccatgttcga acactaatcg aacccggctc
    24601 aaagacccgc tgcggcgggt ttttttgtct gtaatatcca tttgtatgac ctatgcctcc
    24661 ttcacctgcc atttagttga cagatgatgc gctcacggat gaaacattat tgtgaacaaa
    24721 atattttcct cacatgtgat gcctttccgc gctctgatcg acgcttgctg gaaagaaaaa
    24781 tatactgccg cacggtttac ccgtgacctg attgccggga taaccgtcgg gattattgct
    24841 atcccgctgg cgatggcgtt ggctattggt agtggtgtgg ccccccagta cggtttatat
    24901 accgcagctg ttgcggggat tgtcattgct ctaacgggtg ggtcacgctt tagcgtttcc
    24961 ggtccgactg cggcatttgt ggtaattctc tatcccgttt cgcaacagtt tggactggca
    25021 ggactgctgg ttgcgacctt gttgtcggga atctttttga ttctgatggg gctggcacgc
    25081 tttggtcgtc tgattgagta tattccggtt tccgtcacct taggtttcac ctcgggtatc
    25141 gggatcacca tcggtaccat gcagattaaa gattttctcg gtctgcaaat ggcccatgtc
    25201 ccggaacatt atctacaaaa agtcggcgca ttatttatgg cgctgccgac cattaatgtg
    25261 ggtgatgctg ccattggcat tgtgacgctt ggcattctgg ttttctggcc gcgtctgggc
    25321 attcgtttac ccggtcacct tccggccttg ctggctggtt gcgcggtgat ggggattgtt
    25381 aacctgctcg gcggacatgt tgctaccatc ggttcgcaat tccactacgt cctggccgat
    25441 ggttctcagg gtaacggtat tccgcaactg ctgccgcaac tggtgctgcc gtgggatctg
    25501 cctaattcag aattcacgct aacctgggat tctattcgca ccctgctgcc tgcggcattc
    25561 tcaatggcaa tgctcggggc gatcgaatct ctgctctgcg ctgtggtact ggatggtatg
    25621 accgggacta agcacaaggc gaatagtgaa ctggtcggtc aggggctggg gaatatcatc
    25681 gctccattct ttggtggtat taccgctact gctgccatcg cgcgttctgc cgctaacgtc
    25741 cgtgccgggg caacttcccc tatctcggcg gtgatccact ctattctggt tattctcgcc
    25801 ctgctggtac tggcaccact gctctcctgg ctgccgcttt ccgccatggc agccctgctg
    25861 ttgatggtgg cgtggaacat gagtgaagcg cacaaagtgg tcgacttgct gcgtcatgcg
    25921 ccgaaagatg acatcatcgt catgctgctg tgtatgtcac tgaccgtgct gtttgatatg
    25981 gttattgcca tcagcgtggg gatcgtgctg gcatcgctgc tgtttatgcg tcgtatcgca
    26041 cgtatgactc gcctggcacc ggtagtcgta gatgttccag acgatgtcct ggttctgcgc
    26101 gttattggcc cgctgttttt tgctgctgct gaaggcttat tcacggacct ggagtcacgt
    26161 cttgaaggca aacggattgt gattctgaag tgggatgccg ttccggtact tgatgctggt
    26221 ggtcttgatg cgttccagcg ttttgtgaag cgtctgcccg aaggatgtga actgcgcgtg
    26281 tgcaacgtgg aattccagcc actgcgaact atggctcgcg caggcattca accgatcccg
    26341 ggacgcctcg cgttcttccc gaatcgtcgc gcggcgatgg cggatttata agtacgagat
    26401 tgaccagtca gcgcaaactg actggtcagc aaactgcatt atttgttagc tatgacggcg
    26461 attatcgcta cggcggcaac gtttgtcata gtgacgaacc caccagtaac ggtcgcacac
    26521 ctgttcatgc ccgccaacac gggcacccgc cagccagaga atggcaccga cgaaaatact
    26581 tagcactgca ccatgtgcga aatactgggg catattaaac tgtggtaact ggttgaggat
    26641 tgaatacccc acgccgacca ccattaccac cagacccaac cccatgagca cgttaccgag
    26701 tatcgaagcg tttttgcgtt tcatatgtca cctccggaac tttctgggtt gtaacaggga
    26761 atacccctct tccttatgtg taaagtatag acaacaccct gtgggttatg tgcgggcgta
    26821 atcacaatta caacccttat ttcaacaaaa ctttacaaat aaacgcctga acaccttgac
    26881 tttctaatag tccactcagg caattattac gttatctgca tttgatacgc agttttttgt
    26941 caagcggggc cgcaaccagt aaactacgcg ccagttatgt acacactcag gacaaaaaaa
    27001 cgtgacgatt aaattgattg tcggcctggc gaaccccggt gctgaatacg ccgcaacgcg
    27061 acataatgct ggtgcctggt tcgttgactt actggcagag cgtttgcgcg ctccgctgcg
    27121 cgaagaggct aaattctttg gttatacttc gcgagtcacc cttggaggcg aagatgtccg
    27181 cctgttagtc ccgactacat ttatgaatct cagcggcaaa gccgttgcgg cgatggccag
    27241 ttttttccgc attaatccgg acgaaattct ggtggcccac gacgaactgg atctgcctcc
    27301 tggcgtcgcc aaatttaagt tgggcggtgg ccatggtggt cacaatggac tgaaagacat
    27361 catcagtaaa ttgggtaata accctaactt tcaccgttta cgcatcggaa tcggtcatcc
    27421 gggcgataaa aataaagttg tcggttttgt gttaggcaaa ccgcctgtta gtgaacagaa
    27481 gttaattgat gaagccatcg acgaagcggc gcgttgtact gaaatgtggt ttacagatgg
    27541 cttgaccaaa gcaacgaacc gattgcacgc ctttaaagcg caataagtcg ttgtctgcgg
    27601 catttttgcc gagtgccgtg tataataggc aaagttattt ccatttctgc aatctgttag
    27661 caataacagg ttgattatta agatattaag gtgatttaaa tcatgggatt caaatgcggt
    27721 atcgtcggtt tgcccaacgt cgggaaatct accctgttca acgcgctgac caaagccggt
    27781 attgaagcgg ccaactttcc attctgcacc attgagccga acacaggcgt cgtaccaatg
    27841 cctgaccctc gcctggatca actggctgaa atcgtaaaac cgcaacgtac gcttcccacg
    27901 accatggaat ttgtcgatat cgccggtctg gtaaaaggcg catcgaaagg cgaaggtctg
    27961 ggtaaccagt tcctgaccaa catccgtgaa accgaagcga tcggtcacgt tgttcgctgc
    28021 tttgaaaatg acaacatcat tcacgtttcc ggcaaagtta acccggctga cgatattgaa
    28081 gttatcaaca ccgaactggc gctggcagac ctcgacacct gcgaacgtgc gattcatcgc
    28141 gtacagaaga aagccaaagg tggcgataaa gacgcgaaag ctgagctggc ggtcctggaa
    28201 aaatgcctgc cccagctgga aaacgcaggt atgctgcgcg cgctggattt aagcgctgaa
    28261 gagaaagcgg ctattcgtta cctgagcttc ctgacgctga aaccaacaat gtacatagcc
    28321 aacgtcaacg aagatggttt tgaaaacaac ccatatcttg accaggtgcg tgaaatcgcg
    28381 gcgaaagaag gttctgttgt ggttccggtt tgtgctgctg ttgaagcaga cattgccgaa
    28441 ctggacgacg aagaacgtga cgagtttatg caggagcttg ggctggaaga accgggcctg
    28501 aaccgtgtga tccgtgccgg ttataagctg ctgaacctgc aaacttactt caccgctggg
    28561 gtgaaagaag tgcgtgcatg gaccattccg gttggagcaa ccgcgccgca ggcagcgggc
    28621 aaaatccata ctgattttga aaaaggcttt atccgtgcac aaaccatctc gtttgaagat
    28681 ttcatcactt acaaaggtga acaaggcgcg aaagaagcag gcaaaatgcg tgcagaaggt
    28741 aaagattaca tcgtgaaaga tggcgatgtg atgaacttcc ttttcaacgt ctaattaatt
    28801 tattgtctca tgaggtttcg ctaaatctca tgaagagcgc aaaagtatat aaaatccacg
    28861 caattgcgtg gatttttcat ttcaccgagt cctaatcatt cttgtaacat cctacacttt
    28921 tgtgcttatt gcctacagtg aaccacgaga ataattaaag acataagtgt tcagatgact
    28981 gatatctcat tagcagtgat ataacgtcag tttttgttcc actacacatc agccctcttc
    29041 acagctgatc aatattgatc tcgctccaat atgctggcga gaaaaggact tacctgaagg
    29101 gtaatatccc gcagataaag tccccgaata acgtgattac gtgcgatctt ggcaagatga
    29161 aaatgataac cagtagcaca ataataacga taggccagaa aatccattga acaaaaatga
    29221 atatcgatgt tttttgcttt ttcttattag taatgcaaaa atggcgcata ttgtttttgt
    29281 tatgcagata catcaagcga aaatcccata actaattagg attattacaa aaaatcttca
    29341 ccaaattttt taaccgctgt ttgttgtaat atttttgcca tattcatagt aacgaattgg
    29401 aataagtacg acaacttagg gaataactcg atataaactc acctttgtaa ggggggggtt
    29461 aaatatatat taattcctgt gttcctgatt ttgcccggta atagagaatt attctcagag
    29521 aagctcatca tgattgcacc atacagatat catgatgata agaggatatt attcattgac
    29581 ttaaacaaaa tgacaaaatc catagaccca tagcctgggt actatatagg agtggtatgg
    29641 gcatcaaaca acacaatggg aataccaaag ccgatcgtct cgctgaatta aaaatccgtt
    29701 cgccctcaat tcaactgata aaatttggcg ctattggttt gaatgcaatt atcttttccc
    29761 ccctgctgat agctgctgat acaggaagtc aatatggcac caatattact attaatgatg
    29821 gtgacagaat tacaggagat accgccgatc catcaggaaa cctctatggt gtaatgaccc
    29881 cagcaggaaa cacgcctggc aatatcaacc tgggtaatga tgtcaccgtc aatgtcaacg
    29941 acgcctctgg atatgcaaaa ggaatcatta ttcagggcaa aaacagctcc ctgacagcta
    30001 accgactcac agtagatgtt gttggtcaaa cctctgccat cggcattaac ttaattggtg
    30061 actataccca tgctgactta ggcacaggca gcaccattaa gagtaacgat gacggcatca
    30121 ttattgggca tagctcaaca ctaacagcca ctcaattcac cattgaaaac tcgaacggta
    30181 taggcctaac catcaatgac tatggcacca gtgtcgatct tggaagcgga agtaaaatca
    30241 agaccgatgg aagtacaggt gtttatatcg gtggtctcaa cggcaataac gccaatggtg
    30301 ctgcgcgttt tacggcgaca gacctgacaa tcgatgttca gggctacagc gccatgggga
    30361 taaacgtaca gaaaaactct gttgtcgatc tcggaacaaa cagtaccatt aaaaccaatg
    30421 gcgataatgc tcacggcctc tggagctttg gccaggttag cgcgaatgca ctcactgttg
    30481 atgtaactgg agccgcggcc aatggcgtcg aagttcgtgg tggtacaacc actatcggtg
    30541 cagatagcca tatttcttcc gcgcagggcg gtggcctcgt caccagtagt ccagacgcga
    30601 caatcaattt ttctggcacg gcagcgcaac gaaacagcat cttttccggc ggttcttatg
    30661 gtgcctcggc ccagacggca acggctgtta tcaacatgca aaataccgat attacggttg
    30721 atcgtaatgg cagtctggcg ctgggtttgt gggcgctcag cggcggtaga ataaccggag
    30781 acagtttggc tatcaccggc gcggcaggag ccagagggat ttatgccatg accaacagcc
    30841 agatcgacct cacgagcgat ctggtcattg atatgagtac acccgaccag atggccatcg
    30901 caacgcaaca tgacgatggt tatgccgcca gccgcatcaa cgcctcgggt cgtatgctta
    30961 tcaacggtag cgttctttcc aaaggtgggc taatcaatct ggatatgcac cctgggtcgg
    31021 tttggacagg ttcctccctc agcgataatg tcaatggcgg gaaactggac gttgcaatga
    31081 ataacagcgt ctggaacgta acaagtaatt ctaatctcga cacgctggcg ctgagccatt
    31141 caactgtcga ttttgccagc cacgggtcaa ctgccggcac atttaccaca ttaaacgtag
    31201 agaacctgag cggtaacagt acctttatta tgcgtgctga tgttgttggc gagggtaatg
    31261 gcgttaataa tagaggggat ttattgaata tcagcgggag tagtgctggt aatcacgtat
    31321 tggctatccg caaccagggc agcgaggcca caacgggaaa tgaagttctg acagtggtaa
    31381 aaaccactga cggcgcggcc tcgttcagcg cgtcttctca ggttgagttg gggggatatc
    31441 tgtacgatgt gcgtaaaaat ggcactaact gggagcttta cgcttccggg acagttccgg
    31501 aaccgactcc taatcctgaa cccacaccag ctcccgctca gcctcccata gtcaaccccg
    31561 atcctacgcc tgaacccgct cccacgccta aacccaccac gaccgcagat gctggcggca
    31621 attatctcaa tgtcggttac ttattgaact atgttgaaaa ccgtacgctg atgcaacgga
    31681 tgggtgactt gcgaaatcag agtaaagacg gtaatatctg gttgcgcagt tatgggggaa
    31741 gcctggactc ctttgccagt ggcaaactga gcggctttga catgggttac agcggtatcc
    31801 agtttggtgg ggataaacgt ctctctgatg taatgccgtt gtatgtcggt ctgtatattg
    31861 actcaacaca tgcatcgccg gactatagcg gaggcgacgg taccgcacgt tcagactaca
    31921 tgggaatgta cgccagttac atggcacaaa acggttttta cagcgatctc gttataaaag
    31981 catcgcgcca gaaaaatagt ttccacgtac tggacagtca gaacaacggc gttaacgcca
    32041 acggcactgc gaatggaatg agcatctccc tggaagccgg gcagaggttc aacctgtccc
    32101 ctactggtta tgggttctat atagagccgc aaacccagct tacatacagc caccagaatg
    32161 agatggctat gaaggcgagt aatggcctca atatacatct gaatcactac gaatcgctgc
    32221 tggggcgtgc cagcatgata ctggggtatg acatcaccgc aggcaacagc cagctgaatg
    32281 tctatgtgaa gactggcgct atccgcgagt tttcagggga taccgaatat ctgttgaacg
    32341 actcccggga gaagtacagt ttcaaaggta atggctggaa taacggcgtg ggagtcagtg
    32401 cacagtataa caaacagcac acattctatc tcgaagcgga ttacacgcag ggtaacctct
    32461 ttgatcagaa gcaagtcaac ggaggatatc gcttcagctt ttaagtgcgg gatagacaag
    32521 cccttaaacg atgctgaaaa ttgttagcgt gcgccggaat gaaatacaga tcactctgat
    32581 gtatcagaaa tcgtttctgt ctttcatacc cggcgcttaa actgccctgc atcaatgcca
    32641 tgttgcttca ttttccgcca cagcgtagtg cggccgatcc caagtaaagc cgacatttcc
    32701 tgaatgcgac cgcctgtgac ctgggctgcg ttaataattg cctctttttc aacttccgca
    32761 aatgacagac tggtggaaag gcgggtggcg ctgacatcat ctgtcgcctg ctcggtaaac
    32821 agatgttcag gtaaatcact gacgcgaatg cgcccgttat cactactcag agcaagattc
    32881 tcgatgacgc tgtaaagttc aaaatcgttg cctggccatg cacaagaaac taggcgagcg
    32941 agggcatcgt catcaatttt cagccgcgta gagaagcgtt tttcaagact gcgtaatttg
    33001 ttattcacca gcgccggaat gctgccacgc cgcatacgca gaggcgggat ggtaatttca
    33061 aatgcatgca gcgcgtaata cagctggcga ctaaaacgat tttgttccac cagcattgcg
    33121 aggtccacgg tcgttgttgc aatcactttg acatcaattg gtattaaacg ccgcgcatcc
    33181 agtcgcgtga taaccccctg cttgataacc tgcagcaaag cagactgtaa ctccaccgcc
    33241 agatattcaa tcttttcaag aaacagcgtg ccgccgtgtg ccagttccag ccgactcaga
    33301 cggccatttt cattgtccgt gcgatcgcca ccaataaatt cttccgccag cgcagcatca
    33361 ccatataact cacaattgac ggcgatataa ggacctgcag cacgctcgct ttcattatga
    33421 attgcctgac ttagcagtgc cttgcccacg ccctcttctc cacaaagcag gacaggaaag
    33481 ctactgcgcg ccgcctggcg accaaaatga atcaagcggc gggtttgcgg atcgtcctgt
    33541 ggcatatgag cgaaggtatg gctgactttt cctaattgac tggtcattaa ctgccgcatc
    33601 tgttccacag gatggagcaa caaaataaag ctggttccct gcgtttctat tatcggttta
    33661 agggttatca ccgcatcaat aaactggtgc tggctttcaa aggttgcttc tacgtgtttg
    33721 agcggatgtg cctgttttat tgcttgttgc aatacggcgg gtaacgttaa gagttcagtg
    33781 attgcccgtc cctgacttgc cgtcgcgtca aggcgcaaga cccgcgccgc ctgggcatta
    33841 ataaattgca aattaccctg ctcgtcccag ctaatcacgc catcatccat actttctaac
    33901 agggcattaa gttgatttaa atgacggtta gtttcagcga gcaaactgtc cgtcagcagt
    33961 aaattaccga cctcgcgggc gattgccaac gtcaacggca aatcagctgc ggtagtttgc
    34021 tcaaccggac acgccagcgc tattgttccc gtcaatcggc ccttgctgtc aaacaacggc
    34081 gttgcacaaa aggcccagtt ccagagtgcc tgtttgaaat gttgatcggc catcgttttc
    34141 acggcctgac cagagatagc cgctaacgat agcgcacaag taccaataat tccctcggcg
    34201 caatacgtgc cgtcattgaa tcccagtgca cttagctgct gcaaggtttg cggatcgcca
    34261 ttacggctga gaatgcaggc ggtttcatcg aggataaaca gcgcacactc tcgcggtgcc
    34321 atatattccc aggcatcttc cagcgcagcc tgcccgagcg tcagcatcgc tttcttacgc
    34381 cgataaatag aagcaaatgt cacgccctgg gcctgatgtg gtacgttcca tgtctcccgt
    34441 ttcatcagct tattgcatcg ctcccaggag gttgcaatta agggagatat gccccgacca
    34501 tcgttgttaa aagcgccact catatcccgc atcccttatg acaaaaggtg ttccggctcg
    34561 caattttaag tattaataca cgtaaaagtt tcaatatgga acattcatta aagcatatgt
    34621 ttcactatga aacataattt gggctaattt tcttttccct tgcctgatgc acaatggatt
    34681 catcggcagt cagtggtcgc cgtgtcgttg aacatcatcc atgccctacc gtaattgctg
    34741 gagcaaaata atgaaaaaat tgatcaatga tgtgcaagac gtactggacg aacaactggc
    34801 aggactggcg aaagcgcatc catcgctgac actgcatcag gatccggtgt atgtcacccg
    34861 agctgatgcc cctgttgcag gaaaagtcgc cctgctgtcg ggtggcggca gcggacacga
    34921 gccgatgcac tgtggttata tcggtcaggg gatgctttcg ggggcctgtc cgggcgaaat
    34981 tttcacctca ccgacgcccg ataaaatctt tgaatgctcc atgcaaattg atggcggcga
    35041 aggtgtactg ttgattatca aaaattacac cggcgatatt cttaactttg aaacagcgac
    35101 cgagttactg cacgatagcg gcgtaaaagt gaccactgtg gtcattgatg acgacgttgc
    35161 ggtaaaagac agtctttata ccgccgggcg acgcggcgtt gccaacacag tattaattga
    35221 aaaactcgta ggcgcagcgg cggagcgtgg cgactcactg gaggcctgtg cggaactggg
    35281 gcgtaagttg aataatcaag gccactcaat aggtatcgct ctcggtgcct gtaccgttcc
    35341 tgccgcgggc aaaccttctt ttacccttgc ggataatgag atggagtttg gcgtcggcat
    35401 tcatggtgag ccaggtattg accgccgccc tttctcttcc cttgatcaaa ccgtcgatga
    35461 aatgttcgac accctgctgg aacatggctc ataccatcgc actttgcgtt tctgggatta
    35521 tcaacaaggc agctggcagg aagaaccaca aaccaaacaa ccgctccagt ctggcgatcg
    35581 ggtgattgca ctggttaaca atcttggcgc aactccgctt tctgagctgt acggcgtcta
    35641 taaccgcctg accacacgtt gccagcaagc gggattgact atcgaacgta atttaattgg
    35701 cgcgtactgc acctcactgg atatgaccgg tttctcaatc accttactga aagttgatga
    35761 cgaaacgctg gctctctggg acgccccggt ccacaccccg gcccttaact ggggtaaata
    35821 aggagaaagc aatgtcactg agcagaactc aaattgttaa ctggctcact cgttgtggcg
    35881 atattttcaa caccgagagc gagtatctta ccgggctgga tcgcgagatt ggcgacgctg
    35941 accacgggct aaatatgaac cgaggcttta gcaaagtggt ggaaaaactc cctgctatcg
    36001 cagataaaga tatcggtttc attctcaaaa ataccggtat gacgctgctt tccagcgtcg
    36061 gtggtgccag tggtccgctg ttcggtacct tctttatccg cgccgcacag gcgacccagg
    36121 cacggcaaag cctgacactg gaagagcttt atcagatgtt ccgcgatggc gcggacggcg
    36181 taatcagtcg cgggaaagcc gaacctggcg ataaaaccat gtgtgatgtg tgggtgccgg
    36241 tggtggaatc gttacgtcag tccagcgagc aaaatctctc tgttccggtg gcgctcgaag
    36301 ctgccagtag catcgccgaa tccgctgcac aaagtacgat tacgatgcaa gcccgcaaag
    36361 gccgcgccag ttatctcggt gaacgcagta ttggtcacca ggatcccggc gcgacctcgg
    36421 tgatgtttat gatgcaaatg ttggcgttag ccgcaaaaga gtaaggaatt ggtgatggta
    36481 aacctggtca tagtttcaca tagcagccga ctgggagaag gtgtcggtga attagcccgt
    36541 cagatgttaa tgagtgatag ttgtaaaatc gccattgccg cgggaattga cgatccacaa
    36601 aatcccattg gtaccgatgc cgtcaaagtg atggaggcca tcgaatctgt tgctgatgcc
    36661 gaccatgtgc tggtcatgat ggatatgggt agcgcattat tgagtgctga aactgcgctg
    36721 gaattgctgg ctcccgagat cgccgcaaaa gtacgtttgt gtgctgcgcc gttggtcgaa
    36781 ggtacactgg cagcaacggt cagcgcggcc tcgggggcgg atatcgacaa agttatcttt
    36841 gacgctatgc atgcgctgga agccaaacgt gaacaactgg gtttaccgtc ctccgacact
    36901 gaaatctctg acacatgtcc tccgtacgat gaagaagccc gttctctggc ggtggtcata
    36961 aaaaaccgta acggcctgca tgtacgtccg gcctcccggc tggtttatac cttatcgaca
    37021 tttaatgccg atatgttgct ggaaaaaaac ggcaaatgcg tcacaccaga gagtattaac
    37081 cagattgcgt tactacaagt tcgctataac gatacgctgc gcctgattgc gaaagggcca
    37141 gaagctgaag aggcactgat cgctttccgt cacctggctg aagataactt tggtgaaacg
    37201 gaggaagtcg ctccacctac tctgcgtccc gttctgcctg tttcgggtaa agccttttat
    37261 tatcaaccag ttttatgtac ggtacaggca aaatcaaccc tgaccgcgga agaagaacaa
    37321 gatcgattac gccaggctat tgacttcacg ttattagatc tgatgacgtt aacagcgaaa
    37381 gcagaagcca gcgggcttga cgatattgcc gcaatctttt ctggtcacca tacactgtta
    37441 ggtgatccgg aactgctggc ggcggcaagc gaactccttc agcatgaaca ttgcacggca
    37501 gaatatgcct ggcagcaagt tcttaaagaa cttagccagc aataccagca actggatgat
    37561 gaatatctac aagctcgcta tattgatgtg gacgatcttc tgcatcgcac cctggtccac
    37621 ctgacccaaa cgaaagaaga actcccgcag tttaactcgc caactattct actggcggag
    37681 aacatttatc cttccacagt actgcaactg gatccggcgg ttgtaaaagg tatctgcctt
    37741 agcgccggaa gtccggtatc ccacagcgcc ctaatcgccc gtgaactggg gattggctgg
    37801 atttgccagc agggtgagaa actgtatgcg atacaaccag aagaaacgct aacgctggac
    37861 gttaaaacgc aacgtttcaa ccgtcagggt taagccaccg ttcggatggc atcgttctga
    37921 tgtcatccgg caatttaccc agcactgatg ttttttaact acctgttata ttttttacca
    37981 atttatgatg atttttcttc gctaatcgcg aaccaaatgt cgctggctgg ctaataaaca
    38041 gacgttattt gatcactcta tttcgccgtt atttatccct ttaattccct tttctaaaat
    38101 gcctgaccgt tcgcagaatg agatttcgat catgcagcta gtgcgatcct gaactaaggt
    38161 tttctgatac ttgaataccg ttttattcgg tttcgccaaa ggagaatgat tgatgaaatc
    38221 ccccgcacct tctcgcccgc aaaaaatggc gttaattcca gcctgtatct ttttgtgttt
    38281 cgctgcgcta tcggtgcagg cagaagaaac accggtaaca ccacagccgc ctgatatttt
    38341 attagggccg ctgtttaatg atgtgcaaaa cgccaaactt tttccggacc aaaaaacctt
    38401 tgccgatgcc gtgccgaaca gcgatccgct gatgatcctt gctgattatc ggatgcagca
    38461 aaaccagagc ggatttgatc tgcgccattt cgttaacgtc aatttcaccc tgccgaaaga
    38521 aggcgagaaa tatgttccgc cagaggggca gtcactgcgc gaacatattg acggactttg
    38581 gccggtatta acgcgttcta ccgaaaacac cgaaaaatgg gattctctgt taccgctgcc
    38641 ggaaccttat gtcgtgccgg gcggacgttt tcgcgaggta tattactggg acagttactt
    38701 caccatgtta ggacttgccg aaagtggtca ctgggataaa gtcgcggata tggtggccaa
    38761 ttttgctcat gaaataaaca cttacggtca tattcccaac ggcaaccgca gttactattt
    38821 aagccgctcg caaccgccct tctttgccct gatggtagag ttactggcgc agcatgaagg
    38881 cgatgccgcg ttgaagcaat acctgccgca aatgcaaaaa gaatatgctt actggatgga
    38941 cggtgttgaa aacctgcaag ccggacaaca ggaaaaacgc gttgtaaaac ttcaggatgg
    39001 tacccttctc aaccgctact gggacgatcg cgatacgcca cgaccagagt catgggtgga
    39061 agatattgcc accgccaaaa gcaatccgaa tcgacctgcc actgaaattt accgcgacct
    39121 gcgctctgcc gctgcgtctg gctgggattt cagctcgcgc tggatggaca acccgcagca
    39181 gttaaatacc ttacgcacca ccagcatcgt accggtcgat ctgaacagcc tgatgtttaa
    39241 aatggaaaaa atcctcgccc gcgccagcaa agctgcagga gataacgcga tggcaaacca
    39301 gtacgaaacg ctggcgaatg cccgtcaaaa agggatcgaa aaatacctgt ggaacgatca
    39361 acaaggctgg tatgccgatt acgacctgaa aagtcataaa gtgcgcaatc agttaaccgc
    39421 ggccgccctg ttcccgctgt acgtcaatgc ggcagcgaaa gatcgcgcca acaaaatggc
    39481 gacggcgacg aaaacacatc tgctgcaacc cggcggcctg aacaccacgt cggtgaaaag
    39541 tgggcaacaa tgggatgcgc caaatggctg ggcaccgtta cagtgggtcg cgacagaagg
    39601 attacaaaac tacgggcaaa aagaggtggc gatggacatt agctggcact tcctgaccaa
    39661 tgttcagcac acctatgacc gggagaaaaa gctggtggaa aaatatgatg tcagcaccac
    39721 cggaacgggg ggcggcggtg gcgaatatcc attacaggat ggctttggct ggaccaatgg
    39781 cgtgacgctg aaaatgctgg atttgatctg cccgaaagag caaccgtgtg acaatgttcc
    39841 ggcgacgcat ccgaccgtta agtcagcaac gacgcaaccc tcaaccaaag aggcacaacc
    39901 cacaccttaa ccagcgctta ctccgacaac gtcattcggc ttctccggat ggcgttgtcg
    39961 gcagataaca ccatatctat ggttttttct acacagacgg tttttcaagt cctggcggga
    40021 agtattgcgc aaatgtttgc catacgtggt tgccgaagat agcgtcattt ctacgggtaa
    40081 gtgtttcact ttcatcaccc ggcgatttac gccgacgttg gctatataaa gcatcaattg
    40141 ctaccgaata ttgttacggt gttcactcat caacgttttt ttgctttctg gagttaaatt
    40201 aataatcaca taatccagac actccagaga atttagcaac caaaaaaaga attcagacca
    40261 ttcctcttcg cgtaactgaa gcattgccga acgggtattt tggcttaatc tgccagaagg
    40321 attgatcaat tctaaatgtt caataagcgc agccaaaacc gcctcactat ccgccccatt
    40381 tttaacgcct gactggctac tgtctaaaat atttattttc tgaatcacac caaaaacaac
    40441 gaaacaataa ttaaaaaagg attctgttgt tgttaccggg aaccgactta tttcgctcag
    40501 aaagtcgttc aaccagctcc gcagtgataa gattcgctga tgatacagtg tatcctcact
    40561 gcgtgctgga ggtgccagta taatatgttg atcaatacag tatttattgt gttcagtcgt
    40621 ttcctgatgc gtttggttct ctgcggattg tcgctggcga aataaatggc ttgcggtaaa
    40681 tgccgttaca gagcagcgca acgcgtcaat tccttcggag tgcccggctt ttggcgagag
    40741 ttccggtttt atgcgtccag gtagcggaaa tttcacccga atgagaaatg cattaataaa
    40801 atg
//
LOCUS       DAEEUE010000027        40123 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.027, whole genome shotgun sequence.
ACCESSION   DAEEUE010000027 DAEEUE010000000
VERSION     DAEEUE010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40123
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.027"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(24..278)
                     /locus_tag="KLK84_001538"
     CDS             complement(24..278)
                     /locus_tag="KLK84_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBE2008911.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="KLK84_001539"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="KLK84_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HBE2008912.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="KLK84_001540"
     CDS             1228..2694
                     /locus_tag="KLK84_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBE2008913.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /gene="yeiR"
                     /locus_tag="KLK84_001541"
     CDS             2812..3798
                     /gene="yeiR"
                     /locus_tag="KLK84_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HBE2008914.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="KLK84_001542"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="KLK84_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HBE2008915.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="KLK84_001543"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="KLK84_001543"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HBE2008916.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="KLK84_001544"
     CDS             5709..7265
                     /locus_tag="KLK84_001544"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HBE2008917.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILGSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="KLK84_001545"
     CDS             7347..9161
                     /locus_tag="KLK84_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBE2008918.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="KLK84_001546"
     CDS             9162..10256
                     /locus_tag="KLK84_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HBE2008919.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSTFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="KLK84_001547"
     CDS             10256..11281
                     /locus_tag="KLK84_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE2008920.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="KLK84_001548"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="KLK84_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HBE2008921.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="KLK84_001549"
     CDS             complement(12876..13220)
                     /locus_tag="KLK84_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2008922.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="KLK84_001550"
     CDS             complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="KLK84_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HBE2008923.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFYIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="KLK84_001551"
     CDS             complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="KLK84_001551"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HBE2008924.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15616..17376
                     /locus_tag="KLK84_001552"
     CDS             15616..17376
                     /locus_tag="KLK84_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBE2008925.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17501..17785
                     /gene="rplY"
                     /locus_tag="KLK84_001553"
     CDS             17501..17785
                     /gene="rplY"
                     /locus_tag="KLK84_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HBE2008926.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="KLK84_001554"
     CDS             complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="KLK84_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HBE2008927.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19113..19340
                     /locus_tag="KLK84_001555"
     CDS             19113..19340
                     /locus_tag="KLK84_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HBE2008928.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19360..21120
                     /gene="pbgA"
                     /locus_tag="KLK84_001556"
     CDS             19360..21120
                     /gene="pbgA"
                     /locus_tag="KLK84_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HBE2008929.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21195..21271
                     /locus_tag="KLK84_001557"
     tRNA            21195..21271
                     /locus_tag="KLK84_001557"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21229..21231,aa:Pro,seq:ggg)
     gene            complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="KLK84_001558"
     CDS             complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="KLK84_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HBE2008930.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24285..24932
                     /gene="narP"
                     /locus_tag="KLK84_001559"
     CDS             24285..24932
                     /gene="narP"
                     /locus_tag="KLK84_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HBE2008931.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="KLK84_001560"
     CDS             complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="KLK84_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HBE2008932.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="KLK84_001561"
     CDS             complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="KLK84_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HBE2008933.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEKIKPLWEKYSKEAAQ"
     gene            complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="KLK84_001562"
     CDS             complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="KLK84_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HBE2008934.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="KLK84_001563"
     CDS             complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="KLK84_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HBE2008935.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="KLK84_001564"
     CDS             complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="KLK84_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HBE2008936.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="KLK84_001565"
     CDS             complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="KLK84_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HBE2008937.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="KLK84_001566"
     CDS             complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="KLK84_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HBE2008938.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="KLK84_001567"
     CDS             complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="KLK84_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HBE2008939.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTTIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31442..32044)
                     /gene="napC"
                     /locus_tag="KLK84_001568"
     CDS             complement(31442..32044)
                     /gene="napC"
                     /locus_tag="KLK84_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HBE2008940.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32054..32503)
                     /gene="napB"
                     /locus_tag="KLK84_001569"
     CDS             complement(32054..32503)
                     /gene="napB"
                     /locus_tag="KLK84_001569"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HBE2008941.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32500..33363)
                     /gene="napH"
                     /locus_tag="KLK84_001570"
     CDS             complement(32500..33363)
                     /gene="napH"
                     /locus_tag="KLK84_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HBE2008942.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33350..34045)
                     /gene="napG"
                     /locus_tag="KLK84_001571"
     CDS             complement(33350..34045)
                     /gene="napG"
                     /locus_tag="KLK84_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HBE2008943.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34052..36538)
                     /gene="napA"
                     /locus_tag="KLK84_001572"
     CDS             complement(34052..36538)
                     /gene="napA"
                     /locus_tag="KLK84_001572"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HBE2008944.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36535..36798)
                     /gene="napD"
                     /locus_tag="KLK84_001573"
     CDS             complement(36535..36798)
                     /gene="napD"
                     /locus_tag="KLK84_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HBE2008945.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36788..37282)
                     /gene="napF"
                     /locus_tag="KLK84_001574"
     CDS             complement(36788..37282)
                     /gene="napF"
                     /locus_tag="KLK84_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HBE2008946.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37391..37555
                     /locus_tag="KLK84_001575"
     CDS             37391..37555
                     /locus_tag="KLK84_001575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2008947.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            37690..38178
                     /gene="eco"
                     /locus_tag="KLK84_001576"
     CDS             37690..38178
                     /gene="eco"
                     /locus_tag="KLK84_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HBE2008948.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38328..39347)
                     /locus_tag="KLK84_001577"
     CDS             complement(38328..39347)
                     /locus_tag="KLK84_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HBE2008949.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQPVQKEV
                     ADIAL"
     gene            complement(39581..39940)
                     /locus_tag="KLK84_001578"
                     /pseudo
     CDS             complement(39581..39940)
                     /locus_tag="KLK84_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
BASE COUNT         9798 a        10825 c         9895 g         9605 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttgtg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactgggta gctgccttca gtttttagtg gcctggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccgtgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc ggcagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcacccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgcct atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcac tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcacattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgcgg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaacg
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cggcgcactg atgaccatcc
    13861 acgcagccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg taaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg
    14461 ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt cccgccaaga accaccggct
    14521 tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa
    14581 tataagtact gagggtcatc tgcgtactgc ccgccgatac gccaaactgc gctgaaatta
    14641 ccggtagcgc gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa
    14701 ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt
    14761 cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg
    14821 gggctaaatc agcatccagc gtaataccgc caatacgttc acgatgcagc tcaaccacgt
    14881 ggttacccac ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca
    14941 gacgaacctg cgttggggta atcacttcaa gcaccgccgg cttagtgaga tctttttcgt
    15001 tatgcagctg cacgccttta gcaaattgct ctgccgtatc gtcagctaca ggtgattcca
    15061 gtgtcaccag ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact
    15121 gaccatcatc agtcatcagc accagaccgg tggtatcgat atccagacgt cctgcggcgt
    15181 gcagtttcca cgctaccggt tcatccagaa aatagagcac cgtcgggtga tccgggtcgt
    15241 ccgtggagca aacatagccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct
    15301 gcgccagcgg gttgccatcg taagcgacat catgttcagg gagcagtttg aacgctgcat
    15361 tacgaacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag
    15421 cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc
    15481 ctgtaaaaaa cggaggtcgg gtaatgaccc gaaaatctga actgttgtct gcccagtata
    15541 gcggtctaat aacttccctc aagggaaaaa gaaccgtggc atactattag cagaataatc
    15601 tacctacgcg aaaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc
    15661 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca
    15721 ggtaaaagcc tggtgatagc ggaactggca cggctggcgc gtggtcgcgt actggtgctg
    15781 gcacacgtta aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    15841 gccgatattt ttgccgccgg gctaaagcgc aaagagagcc acggtaaagt ggtatttggc
    15901 agcgttcagt cggtcgcccg taatcttgat gcctttcagg gtgaattttc gctgttgatt
    15961 gtcgatgaat gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    16021 cacctgacta aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga
    16081 ttgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa
    16141 gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa acacggctat
    16201 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag
    16261 gcacagagta acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa
    16321 cgcattacgc cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta
    16381 atgattttcg ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa
    16441 gatgctgcac tgattactgg cgacaacccc ggcgctgagc gcgatgtgtt aattgaagat
    16501 tttaaagccc agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac
    16561 gccccgcacg tcgatcttat cgccattctg cgccctactg aatcagtgag tctttaccaa
    16621 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    16681 tatgcgggta atcctcacga tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt
    16741 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    16801 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    16861 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    16921 caatgtaacg cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg
    16981 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    17041 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    17101 tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag
    17161 cgtaccgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg
    17221 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tactgcgaca tccggatttt
    17281 gtcgtcgccc gcatgaaagg tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa
    17341 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    17401 gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg
    17461 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    17521 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    17581 ggcaatcatc tacggtggca aagaagcacc gctggctatc gagctggatc acgacaaagt
    17641 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctaaccatcg ttgttgacgg
    17701 taaagaaatc aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    17761 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    17821 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    17881 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    17941 tccggcgctg caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc aaggtatcgg
    18001 ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    18061 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc
    18121 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    18181 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    18241 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    18301 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc
    18361 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    18421 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    18481 gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    18541 cgttaacgcg catactgctc aggttgctca gaactgccac cagcaaatac tccaccgcca
    18601 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    18661 cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    18721 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    18781 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    18841 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    18901 gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    18961 ttcaaccacc gccagttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    19021 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    19081 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtaatg
    19141 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    19201 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    19261 ccccggccca acgccaggca attgccaact cttttgccag cgccttacag tcctctatca
    19321 acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc
    19381 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    19441 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    19501 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct agtgttcgcc
    19561 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    19621 tttttgtccg tcattctggc gacggcggga atgacgctat tactgatcga tagcgaagtc
    19681 tttactcgtt tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac
    19741 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    19801 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    19861 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    19921 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    19981 ctttcgtacc cgatgacggc gcgacgtttc cttgagaagc atggcctgct tgatgcgcag
    20041 gagtatcaac gccgtcttat tgagcaaggt aatccagacg ccgtttccgt acagtatccg
    20101 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    20161 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    20221 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    20281 ggcctgttct atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    20341 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat
    20401 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    20461 cgcacccaat ctgacgatca gaccgccacg cagtggatca actggctggg ccgctacgca
    20521 caagaagata accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc
    20581 aatcagcagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    20641 aaacgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt ggtgattatc
    20701 actgccggtc ggggtattcc actgagcgaa gaggaagaaa cctttgactg gtcccacggt
    20761 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgct
    20821 ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctgca tgtcagcaca
    20881 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    20941 gttactgctg cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    21001 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    21061 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    21121 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    21181 tagcagccac gagtcggcac gtagcgcagc ctggtagcgc accgtcatgg ggtgtcgggg
    21241 gtcggaggtt caaatcctct cgtgccgacc aaaaatccca agaaaaaacc aacccttacg
    21301 gttggttttt ttatatctgc aattaattcg ataaacagac cgtgacacat cacagcctgt
    21361 ttattttctg ttatcagaac gtccagacca cacccgcctg agtattccac ggcgattcta
    21421 cccctgcgcc attgccatag ctgactgaca aatgaccgct taacgtcggg gtaaacgatg
    21481 accttatccc agcctgataa acgccactgg ttccagccac atcattatta aatttgccat
    21541 cgtcattaac tttcacctgg ttagaatcgg cgtattcctg acgcacggcg gctttcagcc
    21601 agggttccag cgtcgtaccg ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt
    21661 cagcgcgtaa tatccgggta tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt
    21721 cctgaccatc tgtggtaaag ccggtaaagg ccagataggg tctaacactc cacaatccgt
    21781 caacccaacg gaacccgctc tcaacatgag cacccgcgcc gttactattg taatcgccaa
    21841 acgctgttgc cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca
    21901 ccaccccatc aacataggca ccgttctgat gctcccaacc ggcataagcc cccagggtat
    21961 agctatcgat attacctttt ccgccgcgat caaaaccaat atcagaatga gagtaaccaa
    22021 agaacaagcc gcgaattgta ctgctttctt cacgggagaa acggctatcg ataccgagcg
    22081 tcaggcccgt caatgtttgc tcaaaaccag ctcccgcatc agtggtcacg ttgttgcggg
    22141 tgttaattgc cgaactccac atcaccgtat cgtaactaac gccttttacg ctaccaagac
    22201 gctcacgcac ggtgtccagt tctgcatcaa ataccagcgg ttgtgcggct gccatgttca
    22261 gcacatcagt ggttgaaggg gtaatttgcg cgcgattctc tgcgaggctc cagctatggt
    22321 tgccattatc cagcaaggta tattcatacg taccgatatc aacaacgcct ccggcattgc
    22381 ccaacgtaaa tgcagcatca ccgccgcccg ttgttaccag tgtaaggcta tctcctgctg
    22441 ccgggctggc accggtgtcc gtcacgaata ttttgaaatc acctgttgcc tgaccggtga
    22501 cgttgagctg atcgctctga tgattggcca tatcggtacg catataaaaa ttgccgtttc
    22561 ccgacagggt attggtggtc aacgtaatga aattttccgc acttgccgtc acagatgaac
    22621 gagtcactgg ctcagccatc ataatcacat ttgcgccgga catcgcaagg ttggctaagt
    22681 tgaggcgagt gttttggttc gtcgtagtga tatcggcaat gatggtccat gcgctatttt
    22741 cgagcgaaac gtccgctttg ctggtatcca cggcaccatg cgttctaaaa gaagcgccct
    22801 cagaaaccac taccttaccc accaacatag cgggtgcata atttatttgc ggcttcatga
    22861 cctcaagaat gccatcattc cctctgactg acacgttaac cattgtgcca gcccagacat
    22921 tagcgcggcc attgttgatt accatatttt cagagatggc ttttgaggaa tacgtgatac
    22981 ttccattctg gtctgatcga ccgagatcat ataccgcatt tgcctgcact ttcgtaccag
    23041 tatcctttcc cagcgattgc atagtggcat gctcatcaag gatagtgtca atggcctgcg
    23101 tgtcctccat aacaatgagc ccggaaccat catccagttc ataatttttt gacacaccat
    23161 cctttatact aaattggcct ttactgtttg taccactcac ttccagcgca ttcgttgaga
    23221 caattaattt cccgcctgct tttttatcaa cgccagttgc agtcccgcca tccataacct
    23281 ccagtaagcc gccactatcc acagtagtat tataagcgaa gtcattttct tcaactcgca
    23341 aacttccgcc gttttccaac aacatattat tagcaatacc attcttgata tcgaactgac
    23401 cgagacggtt tgttccgaat acctccatgg cccgcgtggt tgttttaata gcaccgcctg
    23461 ctttctgatc taccgcaagc gcaaatcccc cctctttaac ctccagtagt cctccctttt
    23521 cgatagcggc aaaaacggtc cccccatctt tttcaacgat taagcgcccg cctttaatcg
    23581 tggtatcatt cgctgggcca taaacctgta gcacaccatt attgagagta gtctggtttg
    23641 ccgcgccacc catttgaaca atttgataac catcatttaa tactgagtat tctgcggtac
    23701 cattcatttc aatgtattga taccctccgt taatttcagt attactactt acaccaaatt
    23761 cttttatatg ctgttctccg ccaggattga ttttcgtatt attggcggtc ccataaacca
    23821 gctggatatc tttttccagg ataacaccat caactgtctc accatactca actgttgatg
    23881 ccaatgctat gggtgaatat aacgctgtag ccaccagaac agatattgcg gaacgacaaa
    23941 gagaaacaga accagattga tgcattgagc tttcatccta tgaaattaat tgcagttaaa
    24001 agcattgggt acagaaaata cccatagctc catacccgga gtcagttttt aaaaactgtt
    24061 taaagaaatg cacaagtatt gtgattgatt ttttagttgt ttttcttgat gagaagctga
    24121 tgcaaaattc cgtctttata ataaaaatga tgccaaagcg aacgacaagg ttgtagtttt
    24181 cactacatgt ccatacataa aatggggtaa cattcacgcg cctggtagcg ttaccaacgc
    24241 tacgctcaaa cataatgatt ctaataaaac ctcaggagac tactatgcct gaagcaacgc
    24301 cttttcaggt gatgattgtg gatgatcatc cacttatgcg acgcggtgtt cgtcagttac
    24361 tggagcttga tcctggcttt gaagtggtcg ccgaagcggg cgaaggcgcg agcgcgatcg
    24421 atctggcgaa tagactggat atcgacgtga tcttgctgga tctcaatatg aaaggtatga
    24481 gtggcctgga taccctcaat gccttgcgca gggatggcgt taccgcgcaa attattatcc
    24541 tgaccgtatc cgatgcctcc agcgatgtct ttgcgctgat agacgcaggc gcagacggtt
    24601 atctgttgaa agacagcgac ccggaagtat tgctggaagc gattcgtacc ggagcgaaag
    24661 gcagcaaagt ctttagcgaa cgcgtcaatc agtacttacg tgaacgtgaa atgtttggcg
    24721 cggaagaaga tcccttcagc gtgctgacgg agcgcgaact ggatgttctg cacgagctgg
    24781 cacaggggct gtcaaataaa cagattgcat cggtgttgaa tatttccgag cagacagtaa
    24841 aagtacatat tcgcaatctg ctgcgtaaac tcaatgtccg ctcacgcgtg gcggccacca
    24901 ttctgttcct gcaacaacgc ggggcacaat aaaaatagcc cgatggattt atcatcgggc
    24961 tgagatttat gacaaacgca aaactgcctg atgcgctacg cttatcaggc ctacgtggat
    25021 cgatcaattt attgaattta cacaattttg taggccggat aaggcgttca cgccgcatcc
    25081 ggcataaact aagcgcactt tgtcatcagt ctagcccgat ggcatcacca tcgggcctct
    25141 ttttatttac tctcctgcgg cgacaaatgt tgcatcgcct gcgcgatact acgttcaatc
    25201 accgcacggc gagtatcgtt ggcaggtaag agtttcaaca tcatctccca cgcggcaacg
    25261 gcttcgccaa atcgctgctg ctcaaaggca ttaaacgcat acatgcttag cacacggata
    25321 ttgctatggt ccgttctcac cagctgacgt agcagttcac cgccgaggcg gttgtcgttg
    25381 ggatcagatg aacgagtcaa cgcttcagcg tatcccagtg cggcatcgct gttcttcgga
    25441 tcgaggcgat acgcagtagc gtatgcatcg gtggcgatac tggcgttacc cagcgccatg
    25501 ccaacgcggc ccaacataat ccagccttct atatctcccg gatttttttg cagttgagta
    25561 cgcatcccca gcgcaaggcg cgacatctct tcttcgttga gcggatcggc tttcgggtcc
    25621 agcgccctgt ccagcaacgc cggagcctgt gccgtggcct gctgccagat tttcacctgc
    25681 tgataattgc cagtctggta gtagctgacg ccagccacaa ttaacgccac cacaataccc
    25741 ggcagataaa caacatatcc ggcacgctta ccttccggca cgctttgttc aggaaacgct
    25801 tccggcacca cgcgtacccg acgccgcgag cgggcgtata tgacccaacc gccaatgcca
    25861 atagccacta ctggcagcac ccacagcagc acggtcagcg gcgttaacgg cggatcgtaa
    25921 gtgacgaagt tgccgtaacg cgccaccata taatcgacaa tctctttttt acttttacct
    25981 tcctgcatca gttcatacac tttctgacgc aggtcggtgg caatcatcga gttggaatcg
    26041 gcaatgctgt tgttctggca tttcgggcag cgcagttctt cagtgagctg acggaactgt
    26101 tgttcctgtg cttcatcttt aaactgcaac acgtcgatgg tcgccagcgc tgagccggag
    26161 atcatcagca tcagcacgcc caataaaaac ctcattgtgc ggcctcctta ctgtatttct
    26221 cccacagcgg cttgatcttt tcttcccaga cgcgaggatt gagatcgccc gcatggcgat
    26281 agcgaatgat accgttgccg tcaataagga aggtttctgg cgcgccatag acaccgagat
    26341 ccagccctaa catgccgtcg ccatcaaaca ggcttagcgc gtaaggattg cccagctctt
    26401 tcagccagct gattgccttc tggcgatcgt ctttatagtt catgccgacc acgcgaatgc
    26461 cctgcgctga aagctgattc agatattgat gttccgcacg gcaggtcgga caccaagtcg
    26521 cccagacgtt aagcagtact ggtttgccct gagtcagcac atccgcctga taaaactgcc
    26581 ccggattgtc cagtgattcg agacgaaact tcggcacagg cttgccaatg agcgccgatt
    26641 ccagattggt cggatcatcc ccttcggcat tacgcgccag ctgccacagc agcgccgcgg
    26701 caatcgccag gaagataatc aacggaatta acaatacttt gcgcttcata cggcctccgg
    26761 cgcagttttt tgcggactca cgcgcttacg atagcgagga tcaaacagac acagcagtcc
    26821 gcccaacgcc atcatcagcc cgcccgccca aatccagcga acaaatggtt tgtagtaaag
    26881 acgcacggcc cacgcgccgt tttccagctc ttcaccgagc gcggcgtaca ggtcacgcgt
    26941 gatgccgccg tcaattgccg cttcggtcat catcgacccg gcagtgttgt aataacgttt
    27001 ttccgcatac agcaccgttt ccggtttacc ctctcgcgtt acgccaatag tcgccacacc
    27061 gccgcgccag ttcgggccag tcacctcttt gacatcacgg aaggtgaagc gatattcatg
    27121 aatatcgacg ctatcgccgg acttcatgcg cacatcacgc tcaacgctat aattctggct
    27181 aaaggcaatg ccaacaattg tcactgccag ccccaggtga gccgccacca tcccccaata
    27241 actgaaggtg gttttcgtgc cgcgtgaaat acgcagcgca gcttccgcag ttgccagcac
    27301 cgcaatccag caggccattg ccaggccgag caccgtcatc gccacaactt tgctttcgaa
    27361 cagccacggc aacagcagag acagcaccag cgtagagatg aaggcgataa tcaataaatt
    27421 gcggatttta cgtgggcgat cgcgccccca gcgcaccaga ggaccgacac caagcagtag
    27481 cgcaaacggc accatcagcc aggtaaacat ggtgttgaag aacggttcgc caatcgaaat
    27541 actgcccagt ccaagttgct tgtgcaccag cggcagcagc gtccccagca acaccaccag
    27601 catcgcggcg accagcaaaa cgttgttcgc taacagcaaa gattcccgcg accacagcgc
    27661 attgtttacg cgtgagcgaa ctttgtgtcc acgcgcggca aacagcagca gcgaaccgcc
    27721 aatcaccagc accataaagg cgaggataaa cataccgcgc gccggatcag acgcaaacgc
    27781 gtgtaccgat accagcacgc cggaacgcac gaggaaagtc cccaacagac acaacgagaa
    27841 ggcactgatt gccagcagta atgtccacgc tttgaagctg gcgcgttgtt cagtgaccgc
    27901 cagtgagtgc atcagtgcag tccccaccag ccacggcata aacgaggcgt tttctaccgg
    27961 atcccagaac caccagccac cccagccgag ttcgtaatag gcccatgcgg aaccgagcac
    28021 aatgccgagc gtcaggaaaa tccacgccgc cagcgtccac gggcgagtaa aacgcgcata
    28081 agtgctgtcc agacgcccgc tcagcaaaga agcaatggca aaagcaaacg ccaccgagaa
    28141 acccacgtac cccatataaa gcagaggcgg atggaagatc agccccggat cctgtaacag
    28201 cgggttaaga tcgcgccctt caatcgggaa gttcggcagc gtgcgtgaga acgggttaga
    28261 ggtaaagaga atgaacagca aaaagccgac gctgaccatc cccattatcg ccagtacacg
    28321 ggccacaata tccagcggaa tacgctgact aaaaatcgcc accgcaaagg tccagccgct
    28381 catcagcagc acccacagca gaagcgaacc ttcatgcgcg ccccaagtag ccgccacgcg
    28441 ataccacacc ggaagctggg tattggagtt gctggcaaca taggtgacgg taaagtcgtt
    28501 gaccacgaaa gcattgacca gcaccagaaa tgcgccagcc acagacataa acagcagcca
    28561 ggcaaacaag cgggaagacg ccatcatgcg cgcatctccg cgcgccacgc cccatagcgg
    28621 atacacggac agcagcagcg caatccctaa cgccaagcac agcagcccgt ttccaatttc
    28681 cggcatcatg atgctgggtc cttataaaca ctcgccgggc gacggtggtt agcttccatc
    28741 gctttctcaa cttctggcgg cgtgtagttt tcgtcgtgtt tcgccagcac ttctttcgcg
    28801 aggatatgat tgcctttttc cagctcgccc tgcaccacaa cgccctgccc ttcacggaac
    28861 agatccggca aaatgccttc gtaagagaca tccactgagc cttcagcatc gtaaatggtg
    28921 aaggtcactt tcagcgaatt gggatcgcgc tgcacgctac ccggcatcac catcccgcca
    28981 acgcgcagac gctgaccgac ttccggcatc tgctgagttt cacgcttgcc gtagagaatt
    29041 tcccccggcg tataaaagag atcgatattc gagcgcagcg catatagcac cagaccgata
    29101 gtcagcgcta gccctgccaa cacggcacag gcaatccaca agcggttttt acggcgaata
    29161 ttcatgcagc ctcctgctgt tgcgcagcac gtaaacgcgc ctcacgcgcc cgctgttgcg
    29221 ccacaccacg cagaattgcg cgatgttgca tcaccgagtg cacgaccaaa accaccagcg
    29281 gaataacggt catcaccacc gccagccaga caaaaaaggc gtaaccgccc attgcgaaaa
    29341 attcattcca ggaagcaaat gcaggggtca tttacggcct cttttcagta tcagttcact
    29401 cacccacgga cggcgttttt ccatcagcaa aatcaaatta cgcatccgca tcagcgtcag
    29461 cgtggcagac aggagcagga agccaaaaat cgaccagcgc agcggcgaac gcatcgccgg
    29521 atcgatactt tgctgcatcc gcgttgatcc ctgatgcagg gtgttccacc actccacgga
    29581 gtaatgaata atcggcagat tcaccacgcc aatcagcacc aggatacctg ccgcacggcc
    29641 cgccagacgg cggtcgtcga aggcgtgcca cagggcaatc acacccacat acaaaaacag
    29701 cagcaccagt tcagaagtca gacgtgcatc ccatacccac caggtgcccc acatcggttt
    29761 tccccatgca gagccggtaa ccagggcaat aaaggtaaac acggcaccaa tgggggccat
    29821 cgccgccacc gccaggttgg ccattttcat ctgccagaca aggccaataa acgccgccac
    29881 cgccattgat gcataaatgc ccatcgacca gatcgccgca ggcacatgca ggtagataat
    29941 gcggtagcta tttccctgct gataatcagc cggagcaaag ccgaatcccc agatccagcc
    30001 gacggtaagc acgaccacac tggcaattgc cagccacggt ataaaccagc cacagatttg
    30061 atacagccgt ggtgggatcg ccagttgatg cagtgttttc cacatagttt cgataccaga
    30121 ctcgaacaaa aatcagtaat ccagcgttat tgaatgctga ttcgtaacgc tgccgccgtc
    30181 gcaaaaggac ttaatgtcgc ggtgcctgcc agcaacgcgc ctaaaattgc cagataccca
    30241 tcaacgggca aatgcataga agccgcgtcc atcgcggcgg tggcaaagat gagtaatggg
    30301 atagtcagcg gtaacaccag tatgctgagc agcacaccac cgcgcttaag tcccactgtc
    30361 agcgccacgc ccggtgcacc gagaaagcca agcgtaggcg ttcccagcag cagcgtcagc
    30421 gccatcactt gccagccata aacatccatt cccagtagca ttgctaccag tggcgaaagg
    30481 atgagtaacg gcagaccggt taccatccag tgcgccatca ccttcgccag cacaacggcg
    30541 ggcaagggta acggcaacaa catcaattgt tcaagactgc cgtcctgcaa atcgtcacgg
    30601 aacagtcgtt ccagcgccag caaggatgaa agcagcgcag caacccagat aatgcccggt
    30661 gcaatacgcg ccagcagttg cggctccgga ccgatactga gcggaaaaag ggtaattaca
    30721 atcaggaaga accacagcgg gttggcgatt tcggcgctat ggcgaaacgc tacgcgcagt
    30781 tcaagacgga aaatacgcca gaacatcatg cggccctcgt ttgcgtcagt gaaatgcggc
    30841 gaattttact ttcagcaacg ttgagcggct ggtgggtagt cagaatcaca atccccccct
    30901 gctccgtatg ctgcgccata cgctgggtca gacgatcgac gccgttaacg tcaatcgtgg
    30961 taaaaggctc gtcgaggatc cataatgtgg cacgggtcaa ccacagacgc gctaaagcga
    31021 cgcggcgttg ttgcccggca gaaagttgat ttaccggaat atcttcgaat ccggcaagcc
    31081 cggcctgcgc caaggcttcc agacattgtg cggcatcgcc atcgcgatga taaaagtgca
    31141 gattttctaa cgccgtcagc cgggttttga tccccggctg atggcctatc cataacaggg
    31201 tttgatggta gctgtcgcgt acctgatgca agggctgccc ttgccagaga acctcgccag
    31261 cgtcagggcg agacaacccc gtcagcaaac ggagaagcgt tgtcttcccc gcgccgttgc
    31321 taccggtgat ttgtacccac tctcctgcgt tcagcgtaaa tgacaagcca ctaaataagg
    31381 ttcgttcatc ccgctcacaa agtaactctc tggcttcaag catacccacg caataaccct
    31441 gttaaaaacc tggctcgact tcacgcatat cgggcagctt gtgcgcgatc cctttatggc
    31501 aatcaataca ggtttgccca tctttcaccg cctggtcatg catcttcgcg gcaaccgatt
    31561 tctgggcggt tgtatccata tactcgaagt tgtgacagtt acggcactcc tgcgagttat
    31621 tgtccttcat gcgccgccac tcattctgtg ccatcgtcag acgatgagct tcaaatttct
    31681 gcggcgtgtc aataacgcca aaaattttac catacagctc tttacttgct ttgagcttgc
    31741 gtatcatctt cggcacaaac tcgtgcggaa cgtgacaatc cggacaggtc gcacggacgc
    31801 cgctacggtt gttgtagtgc acggaatcca tgtattcctg atacaccgtg ttgcgcattt
    31861 cgtggcagct aatgcagaac tcttcggtat tggctttttc catcccggtg ttaaagcccc
    31921 cccagaagac gatgccgcca acaaaaccga tcaacagcag cgtccccagc gccagatggc
    31981 tgggggtacg ccaccatttc cacaggcgct taatcagacc aggcttacgg tcagaatttc
    32041 ccataataac ctcttatttc ccgtaacctt ttgatggggt aaaggtattc cccacgattg
    32101 gcgcggtatc ggcctgcggt acgtgacatt gcagacagaa ataacgacgc ggagccactt
    32161 ccgcgcccac tttgccgtcg ctgtccataa agtgagtagg actgatacgc ggcgcgccag
    32221 tggtgcgata gctttcgaca ccgtggcact gcaagcagcg attggtattg gtcgttacct
    32281 gataaccttc aacgctatgc gggatcattg gcggctgatt cacatagttc agcggcatcc
    32341 gatcctgctc ttttggcatc cgaatggccc cttcctgcgt cccggatact tccggcgact
    32401 ggctaaaatc gacgccatta gccgcccaaa ccgcgccgct taccaccagg gccagcatcg
    32461 ccgtccattg acacagcgct ttcttcaggt catggctttt catgatttcg ctcccgaact
    32521 ccatcgtgta gttattgtaa atacatcctc agaacagaca tccacgcagc gaccgcacgt
    32581 catgcaatcg cggctggtga cctgcaccgg gctttgttca tccagcaccg gggcacgtag
    32641 cacatgcggt tccgggcaaa catgaaaaca atccatacag cggttacatt tctgccgatc
    32701 gctggccgca acggtaatca cacctttgct acccagcacg ccatacagcg cgccaaccgg
    32761 acaaatgtgc ccgcaccagc cgtgttcaac gaccagtaga tcaaataaaa acagcgcgag
    32821 aataagcagc gcgccgctgc cgaagcccat taccaggcta cgccccatca gagaaacggg
    32881 gttgatccat tcccaaatga gcgtgccagt taaggctgat cccaccagaa tgaccaccaa
    32941 cagcacgtag cgaatgtggc gagggatcgt cgcagactga ttgaggtcaa agcgcctgcg
    33001 taaccagttc gctaagtcgg taatcgggtt cagcgggcag acccagctgc aaaataatcg
    33061 ctttcccgcc agggcgtaga gcacggtgat aatcaccgca ccggtcaacg ccaccgtggc
    33121 aggcagatga ccactggcaa gactttgcag cgtcatcagc ggatcggtca gcggaacggt
    33181 gtcgaataac aggctactgc tgtagttgcc gtgcaagatc cacacaccaa accatggacc
    33241 gctcaaaaac atccccagca cgaagaactg acaaaggcga cgtaacacca gccaacggtg
    33301 actgcgccac cagccttttt tctccagcgc ctcgcgcccg gcgtcacgtt tacgatttgc
    33361 cattgttccc ctccagccag ccgaagcggt aatggtgacc taactcccct ttcgccagtg
    33421 acagcggtaa caccttgatt gccggttgtt ccagcacgca tactttttcg cacttaccgc
    33481 aaccggtaca ggcgtcgctg tgaaccgtcg gcagaaagcg ggcgtgctta ccggtacgcg
    33541 tgttgcgctc cagctccaag gtgatggcct catcaatttt cgggcattcg cgataacaaa
    33601 catcgcagcg cagcccctga aagttgagac agttttcctg gtccaccagt accgccagcc
    33661 ccatccgcgc gtcgtcgatc gattcaatct cacgatccag cgcaccgctt gggcacactt
    33721 tggcgcacgg aatgtcctca cacatttcgc aaggaatatc ccgtgcgaca aaatatggcg
    33781 tgcccgccga cagaccagag gccagcgtcg ccagttttaa ggtgtcgtaa gggcaagcct
    33841 gaacacactg accgcaacgc acacaggcac tggcaaaggc gttctcgttt atggctccgg
    33901 gcgggcgcaa ccgcacgcca gatgcgcgtg cggtttgctg ttgtaacccc agcgccacac
    33961 ccacggcagc cagcccgcct gctgtgcgaa cgacatcgcg cagaaagcgg cggcgaccat
    34021 tttgaggttt cgctgaccgg gacataatgg cttacacctt ctccagtttg accgcgcact
    34081 tcttgaagtc cgtctctttc gagagcggat cggtcgcatc cagcgtcagt ttgttaacca
    34141 gctgtgcggc gtcgaagaac ggcatgtaca ccaggccctg tggcggacgg ttacgaccgc
    34201 gcgtttcaac aatcgagatc acttcgccac ggcgagaaac cactttcact ttgtcgccac
    34261 ggcgcagatc gcgcgctttc gcatccagcg ggtgaataaa caagaccgct tccgggaagg
    34321 cgcggtgcag ttccggcaca cggcgagtca tactgccggt gtgccagtgc tccagaacgc
    34381 gtccggtaga gagccacagg tcgtactctt catccggtgc ttccgccgcc ggttcgaacg
    34441 gcagtgcgaa gatcaccgct ttgccatccg gtttaccgta gaacttatag ccttcgcccg
    34501 ctttcacgta cgggtcgtta ccttcgctgt aacgccactg tgtttcttta ccattcacca
    34561 ccggccagcg cagaccgcgc gctttgtggt agtcatcgaa cggtgccaga tcgtgaccgt
    34621 gaccgcgacc aaaccatgcg tactcttcga acagcccttt ttgcagatag aagcccagct
    34681 cgcgggattc atcgttcagc tgatcttccg ccagttcgga taccgggaat ttgctcactt
    34741 cgggggtggc atacagaact tcgtacagcg ttttgccacg cagttccggt ttcttcgcca
    34801 gcagctcttc cggccatact tcttcagttt tgaagcggcg ggagaactgg actaactgcc
    34861 agagatccga tttcgcttcg cctggcgcct gtacctgctg acgccagaac tgagtacggc
    34921 gttcggcgtt accgtaagcg ccctctttct ctacccacat tgcggtcggc aggatcaagt
    34981 cggcggccag cgcactgact gtcggatacg gatcggagac gatgatgaag ttgcgcggat
    35041 cgcgccagcc cggcatacgc tcttcattga tgttcggccc ggcctgcatg ttgttggtac
    35101 acatggtcca gtaaacattc agcttgccgt ctttcagcgc acggtcttgt gccaccgcat
    35161 gaagaccgat tttcgccgga atggtgccgc tcgggatatt ccacttcttc tcacagatat
    35221 cacgatgttt ctcgttagtt accaccatgt ccgcaggcag acggtgagca aaggtgccca
    35281 cttcacgcgc agtaccacac gcagaaggtt gcccggtcag ggagaacgga ccgcaacccg
    35341 gctgggaaat tttgccggtc agcaggtgca ggttgtagac caggttgtta gcccacacac
    35401 cacgggtatg ctggttgaag cccatcgtcc agtaggagat gactttcttg ttcggatcgg
    35461 catacagctg cgccagttgt tctaactggt ctttcggcac gccggtcatt tcagcggttt
    35521 tttccagcgt atactcagca acgaaggctt tgtaatcttc aaagctcatc ggttcggagg
    35581 cgtcagaacc cggattcttc gctgcttttt ccagcggatg ggtcggacgt aaaccgtagc
    35641 cgatgtccgt cgcccctttg cgcaggttaa cgtgcttgct gaagaagtcc tgatttatcg
    35701 cattgttttg aatgatatag ttggcgatgt agttcaggat caccaggtca gattgcggcg
    35761 taaagatgat gccgttatcc gccagctcga agctacgatg ctggtaggta gaaagcaccg
    35821 ccacggtgac gttctggtta gagagacgac tgttagtgat gcgtgaccag aggatcgggt
    35881 gcatctccgc catgttagcg ccccacagca caaacgcgtc agcctgctcg atgtcgtcat
    35941 agcagcccat cggctcatcc ataccaaagg tacgcataaa gccaactact gccgacgcca
    36001 tacagtgacg tgcgttcggg tcgatgttgt tcgaacggaa gcccgctttg aacagcttgg
    36061 acgcggcata accttcccag atagtccact gaccagaacc gaacataccg atcgattccg
    36121 gccctttttc tttcagggcg gttttgaatt tctcttccat cacatcgaag gcctgatccc
    36181 aggtgattgg ggtaaattcg ccttctttgt catatttacc gtttttcata cgcagcagcg
    36241 gctgcgtcaa acggtcttta ccgtacatga tcttgggcag gaaatagccc ttaatgcagt
    36301 tcaggccacg gttaaccggt gcgtccgggt cgccctgaca ggccaccaca cgcccctgct
    36361 gcgttccgac cagaacgccg caaccagtac cgcagaaacg gcacggcgct ttatcccatt
    36421 taatggcttc ctgctgacca acaacggcgc gggcaacgcc cggcacgctg agaccggcag
    36481 ccgccgcagc ggccgcaacg gcgttagctt tcataaagct acgacgactg agtttcatgg
    36541 tgtttcctca ccttgctctt cctgctggtg ataaaccagc gacaccgcca gcacgccctc
    36601 tacgttgcgt actgactcaa tggtttggat cagcgtttcg ctgtcttctg cttccaccac
    36661 cacaatcaac tgaccgctcg gcgcgtcgct gacagcaact tcacagccgg gaaaggcgtt
    36721 cagttgggtg ctgatgtctg aaattcgttc gcttttggcc tgcacgacca ggctgcaaac
    36781 ttgccagtta gtgtgcatgg agatactccg cagttatggc tgataccgga cagctggctg
    36841 cacatgctcc acatccgttg caaagttggc tattaagttg cgcctggtag atcccggaga
    36901 gcgtcgggcg aaagataatt gccattggct cacagctatc ctgacagcgg cggcattcaa
    36961 ctgactgata cgccaggcac gcgtccccga tggtgaactg caaatcccag gccctggtgt
    37021 ggcgcggaga aaatagcgat tcggggcaag cctgcgcgca ggcgtaacag aagctgcact
    37081 cgttattttt gaaattaacg ctcggatagc cacccgtgcc gcgttgcaga atgttgtttt
    37141 cacaagcatt aatacaggcg tcgcagcggg tgcaatgggt cagaaaatga gattcatcac
    37201 cggaccaggg cggacggata ccgttactgg ctttgcgcca gcaaccagtg agtatgcccc
    37261 gacgggatgc atcaatcttc acattgacct tccatcatta acgcgctctg aaaattgaga
    37321 gcgaccaaat agaccgcata attaataagc catttttata gccgctaaga tattaaagga
    37381 tgtgtcaaag atgcataccc cgatcggggt aaaacctgta gcaggatcaa aagagtggcg
    37441 ggaagcgtgg caaaaacggg cttttgctca catttcaaat ggttataaac atatttatat
    37501 agcgattaat tcaccagaga tatttctgct ggtttgctcc ctcatcagaa tttaacacta
    37561 aaagagcagg taaaattgtc tgaatgttct ttaagttatt cataaagcaa attaataaat
    37621 ctgatgaata tgttaacctt cagcgacatc atcggtgaaa acctataaat gaagaaggaa
    37681 agcaaaaaaa tgaagaccat tctacctgaa gtattgtttg ccgctttcgc taccacttcc
    37741 gcctgggcgg cagaaagcgt ccagccactg gaaaaaatcg cgccttatcc acaagctgaa
    37801 acaggcatga agcgtcaggt gattcagtta accccgcaag aagatgaatc taccctgaaa
    37861 gtagaactgt taatcggtca gacgctggaa gtcgattgca atttgcatcg tctcggcggg
    37921 aagctggaaa gcaaaacgct ggaaggctgg ggctatgatt attatgtctt tgataaagtt
    37981 agctcgccgg tttccacgat gatggcctgc ccggatggca agaaagagaa gaaatttgtc
    38041 accgcgtatc tgggcgatgc tggaatgctg cgttacaaca gcaagctgcc gatcgtggtg
    38101 tatacgccag acaatgtaga tgtgaaatac cgcgtctgga aggcggaaga gaaaattgac
    38161 aacgcggaag ttcgctaaac tgttgtgaag tacggcaccc cgttggttgg ataaggcggt
    38221 tacgccgcat ccaacatcta acgcccgagc cggttgcctg atgcgacgct ggcgcgtctt
    38281 atcagtatac gccacatccg gcataccatg ccggatgtgg cacatcatta caacgcaata
    38341 tccgccactt ctttttgaac gggttgcggt ttcggcgtac tatctgctgc ttgcggtttg
    38401 tcgtatttca gccccagcac ttcgctggtg tactgcaact cgcgttctgt tgccgctaca
    38461 tcaccgttca gcttgcgtcc ataagacgga acgatcgctt tcaacgtagc ctgccattgc
    38521 gggctggaaa cgcgatcgcc aaatactttt tccagtagat tcaacataat cggcgcggcg
    38581 gttgacgccc ctggcgatgc ccccagcagc gccgcaatgg tcccttgctg gtcactgacc
    38641 acttcggtac ccagacgcag tacgccacct ttctcggcat cacgcttgat aatctgcaca
    38701 cgctgccccg cttgccacaa acgccagtcc tcttttttcg cttgcggata gtactctttc
    38761 aacgcttcaa aacgatcctc ttcactcaac atcacctgac tcaccagata tttcaccaga
    38821 tcgaaattat ccagcccgac gtgcatcatc ggcatcacgt tagaggtggt ggtggaactc
    38881 atcagatccc acaatgaacc gtttttgagg aatttggttg agaaggtggc aaatggccca
    38941 aacagcacta cgcgtttacc gtccagaaca cgggtatcga tatgcggaac cgacatcggt
    39001 ggtgcgccaa cggatgcttt accgtaaact ttcgccagat ggcgattaac cacgtctgga
    39061 ttttccgaaa caaggaactg tccgcccacc gggaaaccgg cgtagtcttt cgcttccgga
    39121 atccccgatt cctgtaacag cttcagcgcc gcaccgcccg cgccgataaa gacaaatttg
    39181 gcgcggatgt tctgtgcagt gccgtttttc agatcggcaa cggtaacggt ccaggtgtta
    39241 tcgtcattac gctttagggc gcggacttcg ctgctgagtt gcagcgagaa gttagatttc
    39301 ttctgtaagg aagcaattaa ctggcgggtg atctcaccat agttcacatc agtgccaatt
    39361 tctgttgcgg atcgcgccct tccatcacta acggtgccca ctctttgatc tgcgcgtgat
    39421 cttcagagta acgcataccg cgaaacagcg agctttgttg caacgcggcg taacgggcgc
    39481 gcaggaaatt aacgttatcc tcgccccaga caaagctcat atgcggaacg gtattgataa
    39541 atgaacgcgg agtacgcagc acgccgcgct cgacctggtg cgcccagaac tggcgggaaa
    39601 tctgaaatgc ttcgttaatg gcgactgctt tttcaatact gatgctgcca tcggcgtttt
    39661 gcggggtgta gttcagttcc atcagtgcag aatgcccggt tccggcgttg ttctagccgt
    39721 tcgaactctc ctgcgcgccc ccctccaggc gctccaccat ggtcatcgac cattcaggct
    39781 ccagctcgcg tcaataggtc cccaacgtgg cgctcataat gccgccgcca atcaacagta
    39841 catcagtttc ctgctcctcg gacgctttcg cttttgccgc catcgaaacg gcattaagcc
    39901 ccacggccat cgagaagagc atggcagtca cttttttcat cttgttaatg ccttactttt
    39961 agtcgcttta ttgcaggtga gatttgcgcg gcatcaacgg taacacttaa gtaacaacat
    40021 ttaaataatg tttataaatt atattctaat tttagaaaca ttataatttc gttgattaat
    40081 tatagggtta ttagaaatga agggattttt agcatattga cct
//
LOCUS       DAEEUE010000028        38531 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.028, whole genome shotgun sequence.
ACCESSION   DAEEUE010000028 DAEEUE010000000
VERSION     DAEEUE010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38531
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.028"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..74)
                     /locus_tag="KLK84_001579"
     CDS             complement(<1..74)
                     /locus_tag="KLK84_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HBE2008950.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLV"
     gene            complement(172..1281)
                     /gene="anmK"
                     /locus_tag="KLK84_001580"
     CDS             complement(172..1281)
                     /gene="anmK"
                     /locus_tag="KLK84_001580"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBE2008951.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1555..2022
                     /gene="slyB"
                     /locus_tag="KLK84_001581"
     CDS             1555..2022
                     /gene="slyB"
                     /locus_tag="KLK84_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HBE2008952.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="KLK84_001582"
     CDS             complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="KLK84_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HBE2008953.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2704..2940
                     /locus_tag="KLK84_001583"
     CDS             2704..2940
                     /locus_tag="KLK84_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HBE2008954.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2943..3800
                     /locus_tag="KLK84_001584"
     CDS             2943..3800
                     /locus_tag="KLK84_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBE2008955.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3800..5797
                     /locus_tag="KLK84_001585"
                     /pseudo
     CDS             3800..5797
                     /locus_tag="KLK84_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5798..6319)
                     /gene="sodC"
                     /locus_tag="KLK84_001586"
     CDS             complement(5798..6319)
                     /gene="sodC"
                     /locus_tag="KLK84_001586"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HBE2008956.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6400..7296)
                     /locus_tag="KLK84_001587"
     CDS             complement(6400..7296)
                     /locus_tag="KLK84_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HBE2008957.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7345..7584)
                     /locus_tag="KLK84_001588"
     CDS             complement(7345..7584)
                     /locus_tag="KLK84_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HBE2008958.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7687..8286
                     /gene="nemR"
                     /locus_tag="KLK84_001589"
     CDS             7687..8286
                     /gene="nemR"
                     /locus_tag="KLK84_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HBE2008959.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8323..9420
                     /gene="nemA"
                     /locus_tag="KLK84_001590"
     CDS             8323..9420
                     /gene="nemA"
                     /locus_tag="KLK84_001590"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HBE2008960.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9501..9908
                     /gene="gloA"
                     /locus_tag="KLK84_001591"
     CDS             9501..9908
                     /gene="gloA"
                     /locus_tag="KLK84_001591"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBE2008961.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10011..10658
                     /gene="rnt"
                     /locus_tag="KLK84_001592"
     CDS             10011..10658
                     /gene="rnt"
                     /locus_tag="KLK84_001592"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HBE2008962.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10751..15209
                     /locus_tag="KLK84_001593"
                     /pseudo
     CDS             10751..15209
                     /locus_tag="KLK84_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(15253..16461)
                     /locus_tag="KLK84_001594"
     CDS             complement(15253..16461)
                     /locus_tag="KLK84_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE2008963.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            16469..16969
                     /gene="tnpA"
                     /locus_tag="KLK84_001595"
     CDS             16469..16969
                     /gene="tnpA"
                     /locus_tag="KLK84_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBE2008964.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     THRIKD"
     gene            complement(17008..17355)
                     /gene="grxD"
                     /locus_tag="KLK84_001596"
     CDS             complement(17008..17355)
                     /gene="grxD"
                     /locus_tag="KLK84_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HBE2008965.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            17690..18517
                     /gene="mepH"
                     /locus_tag="KLK84_001597"
     CDS             17690..18517
                     /gene="mepH"
                     /locus_tag="KLK84_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HBE2008966.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPHTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            18645..19226
                     /gene="sodB"
                     /locus_tag="KLK84_001598"
     CDS             18645..19226
                     /gene="sodB"
                     /locus_tag="KLK84_001598"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HBE2008967.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19372..20541)
                     /locus_tag="KLK84_001599"
     CDS             complement(19372..20541)
                     /locus_tag="KLK84_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE2008968.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(20707..20796)
                     /gene="ynhF"
                     /locus_tag="KLK84_001600"
     CDS             complement(20707..20796)
                     /gene="ynhF"
                     /locus_tag="KLK84_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HBE2008969.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            21095..22120
                     /gene="purR"
                     /locus_tag="KLK84_001601"
     CDS             21095..22120
                     /gene="purR"
                     /locus_tag="KLK84_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HBE2008970.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(22117..23049)
                     /locus_tag="KLK84_001602"
     CDS             complement(22117..23049)
                     /locus_tag="KLK84_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE2008971.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            23162..24373
                     /locus_tag="KLK84_001603"
     CDS             23162..24373
                     /locus_tag="KLK84_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HBE2008972.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            24664..25812
                     /gene="cfa"
                     /locus_tag="KLK84_001604"
     CDS             24664..25812
                     /gene="cfa"
                     /locus_tag="KLK84_001604"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HBE2008973.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(25852..26493)
                     /gene="ribE"
                     /locus_tag="KLK84_001605"
     CDS             complement(25852..26493)
                     /gene="ribE"
                     /locus_tag="KLK84_001605"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBE2008974.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            26708..28081
                     /gene="mdtK"
                     /locus_tag="KLK84_001606"
     CDS             26708..28081
                     /gene="mdtK"
                     /locus_tag="KLK84_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HBE2008975.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(28122..29378)
                     /locus_tag="KLK84_001607"
     CDS             complement(28122..29378)
                     /locus_tag="KLK84_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HBE2008976.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            29686..29762
                     /locus_tag="KLK84_001608"
     tRNA            29686..29762
                     /locus_tag="KLK84_001608"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29720..29722,aa:Val,seq:gac)
     gene            29767..29843
                     /locus_tag="KLK84_001609"
     tRNA            29767..29843
                     /locus_tag="KLK84_001609"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29801..29803,aa:Val,seq:gac)
     gene            29951..30256
                     /locus_tag="KLK84_001610"
     CDS             29951..30256
                     /locus_tag="KLK84_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBE2008977.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            30382..31986
                     /locus_tag="KLK84_001611"
     CDS             30382..31986
                     /locus_tag="KLK84_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HBE2008978.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(31998..32810)
                     /locus_tag="KLK84_001612"
     CDS             complement(31998..32810)
                     /locus_tag="KLK84_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2008979.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(32814..33599)
                     /locus_tag="KLK84_001613"
     CDS             complement(32814..33599)
                     /locus_tag="KLK84_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HBE2008980.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(33596..34264)
                     /locus_tag="KLK84_001614"
     CDS             complement(33596..34264)
                     /locus_tag="KLK84_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBE2008981.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(34328..34966)
                     /locus_tag="KLK84_001615"
     CDS             complement(34328..34966)
                     /locus_tag="KLK84_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HBE2008982.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(34979..37080)
                     /locus_tag="KLK84_001616"
                     /pseudo
     CDS             complement(34979..37080)
                     /locus_tag="KLK84_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(37101..37727)
                     /locus_tag="KLK84_001617"
     CDS             complement(37101..37727)
                     /locus_tag="KLK84_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HBE2008983.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(38183..38392)
                     /gene="fumD"
                     /locus_tag="KLK84_001618"
     CDS             complement(38183..38392)
                     /gene="fumD"
                     /locus_tag="KLK84_001618"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HBE2008984.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         9280 a        10058 c         9432 g         9761 t
ORIGIN      
        1 accagttgat taaaggcgct acaaccggag agaaggaccg gcaaaatgat aattaacagt
       61 tttttcatag tcatatcccg aagactttcc tggtccggag ggcaaatacc ctccttaatc
      121 ttccaagtgt aacggcagac acgataagaa aaaattcagt taattctgat atcacgagtt
      181 ggcggggaaa atagccccca gtaccgtctc ctggcttgcg ccagtgacgg aaggcagatt
      241 tcctggtaat cccgccagcg tccgccaggc aagccaggcg aaagccaatg cttccatgtc
      301 atcgccacta atgccaacgg catcggtggt ggtgacttct gtgcctagca gtaataccgc
      361 cagacgcgcc atgagtagcg gattacgact acctccacca cataccatca atcgttcgca
      421 gccaccgctc aacaaaactt gttcagaaat ggtcacggcg gtgagttctg ccagtgtggc
      481 ctgaacatca cggggatcaa cacccggaaa atggcgtaaa tggcgctcca gccagccgta
      541 gttaaagtat tcgcgtccgg tgcttttcgg tgcaggttgc gagaaatacg ggtcgctgag
      601 catattttgc agcagtggga gaataacttt acccgcccgt gcccactcgg catctttatc
      661 gtaaggttta cctgcctgac gccagatcca ggcatccatc agcatgttac caggaccggt
      721 atcgtagccg ccaaccggct gcccaggaat gagcagtgac agattggcga tgccgccaat
      781 attgagcacc attcgtcgct cggttgggtg agccagcaag gcatgatgga acgcaggtac
      841 cagcggtgcg ccttgtcctc ccaaggcaat atcgcgacgg cgaaaatcac cgacaaccgt
      901 aattccggtg cgtgccacaa tttgattgtt atcgccaatc tgcaaagtgt gtggtgccac
      961 gcccgtcggt tcatgccaga cggtttgacc gtgacagccg atcgcaacta tatctctcgc
     1021 ctgcaggttt tgttccttaa gcaaggcatt gaccgcatca gcaaaaagtc gcccgagttg
     1081 agtatcaagc tgtccaaact gcgaaagtgt aagctgctgg ccctggcaaa tatccagtac
     1141 agcctgtttc agagacaccg ggatcggcca gctcaagctt gccagctgtg cgacccggtg
     1201 ttcatcaatt gtcgccaaca caacatcaac accatcaagg ctggtgcctg acataacgcc
     1261 aataaagcgg cccgatttca tagttcatcc tttttcaatc tgacgtttgc gcaccactca
     1321 aacataaact tttcgtgaat accatgcgga atgaccgatt tttaccgttg gtagtaaaac
     1381 attatcttca aatcaataat catcatgaat gttttgttta taattggttg atcctacttt
     1441 cattatgatt tgctcatatt tggtagaaca tgtaaccatg gattcacata tgccatatac
     1501 tttgaccatg agggatgctt gcgtggcgtt tcatggtgaa caggagattt ttcaatgatt
     1561 aaacgcgtat tggttgtttc aatggtaggt ctgtctcttg tcggttgtgt taataacgac
     1621 accctgtcag gggatgttta taccgcttct gaagcgaaac aagtacagaa tgtcagctat
     1681 ggcaccatcg ttaacgtacg tccggtacag attcagggcg gtgatgattc caacgttatc
     1741 ggtgcaattg gcggtgctgt tcttggtggt ttcctgggga atactgttgg tggcggaacc
     1801 gggcgttctc tggctactgc agcaggcgct gttgcaggtg gcgtagctgg tcagggcgta
     1861 cagagtgcaa tgaacaaaac gcagggtgtc gagctggaaa ttcgtaaaga cgatggtaat
     1921 accatcatgg tggtacagaa acaaggcaac actcgtttct ctccgggcca acgtgtcgta
     1981 ctggccagca atggcagtca ggtgaccgtt tctccgcgct aaataagttt gcgtgtggtc
     2041 aggttactga ccacacgccc ccttcatttc accctttggc ctgtaactca atgatattat
     2101 gctcaagttt tgcgatgagc gtaatcagtt gctccagttc ctctgcggag atgccatgta
     2161 atatttccgc gcgggttttg ttaataacag cttccatttc gctgatcagt ggctctgcct
     2221 tttccgtcag tttaatacgt ttagcccgac gatcgctggc acaagtttga cgcgaaatta
     2281 accctttttc ttccagttgg tccagagtac ggaccagtga tggctgctcg atgccaatcg
     2341 cttttgccag ttgaatttgc gactggtctg gaggtaactg atggatattg tgtaacgtaa
     2401 cccaatgggt ttgtgttaac tccagcggtt tcaggcgatg gtctatcaga gcacgccata
     2461 tgcgcaccaa ccgtgccaga tcagaaccta gtggcgattc caatttcatc tccttataat
     2521 tagcttgcta agatattatg cggcttttag aatagtgtgc agcaattgta ttgctaaaac
     2581 aaatgtattg ctgcatttgg ttaccgtcag acatattttt cagaaattgc gcgtaaattt
     2641 ttcgcactta aagaatattt attaatctaa cgcaatatat tcggtcgtaa aggaatctac
     2701 tttgtgaagt ttatgctcaa tgcaacagga ttgcccttac aagatctggt gttcggtgcg
     2761 tccgtctatt ttcctccgtt tttcaaagca ttcgcgtttg gattcgtcat ctggcttgtc
     2821 gtacaccgcc tgcttcgtgg ctggatctac gccggcgata tctggcatcc cttgttaatg
     2881 gatttatcgc tgtttgcgat ttgcgtttgc cttgctctgg caatactgat tgcgtggtaa
     2941 ctatgtcaat taaaacaatt aagtatttct caacaatcat tgtagcgata gttgcggttc
     3001 ttgccggatg gtggctgtgg aattattaca tgcaatctcc gtggacacgc gacgggaaaa
     3061 tacgcgcaga acaggtttct atcaccccac aggtgtcggg acgtattgtt gagctgaata
     3121 taaaagacaa ccaactggtt aacgcggggg aacttctgct cacgatcgat aaaacgccct
     3181 ttcagatcgc cgagctgaac gcacaggctc agctggcgaa agcgcaatct gacctcgcaa
     3241 aagccaataa cgaagcgaat cgtcgtcgtc atctctcaca aaattttatt tttgccgaag
     3301 aactcgatac cgctaacctc aatgttaaag cgatgcaggc cagtgttaat gccgcacagg
     3361 cgacgctgaa acagacacaa tggcaactgg cgcaaacgga aattcgcgcc ccggtgagtg
     3421 gatgggtgac taacctcact acccgcatcg gcgattacgc tgacaccggg aaaccgctgt
     3481 ttgcccttgt cgacagccac tcgttttacg tcattggtta ttttgaagaa accaagttgc
     3541 gccatatccg cgaaggtgca ccagcacaaa ttaccctcta tagtgataac aaaacgttac
     3601 agggtcacgt ttccagtatc ggtcgggcga tttatgatca gagcgttgaa agtgactcaa
     3661 gccttattcc ggatgtgaaa cctaacgtcc cctgggttcg tctcgcccaa ctcgttcccg
     3721 tccgctttgc gctggataaa gttccaggcg atgtcacgct ggtgtcgggc accacctgta
     3781 gcattgccgt aggtcaataa tgaacgcatc gtcatggtcc ttgcgcaatt tgccctggtt
     3841 cagggccacg ctggcgcaat ggcgttatgc gttacgcaat accattgcca tgtgtctggc
     3901 gctgacggtt gcctattatt tgaatctgga tgaaccctat tgggcgatga cctcggctgc
     3961 agtggttagc tttcccaccg ttggcggtgt catcagcaaa agcctcggac gcatggctgg
     4021 cagtttactg ggagccattg cggcactgct tcttgccgga catacgctca atgagccgtg
     4081 gttttttcta ttgagcatgt cggcgtggct tggcttttgt acctaggcct gtgcgcactt
     4141 cacgaataac gtcgcgtatg catttcaact ggcgggctac acggctgcca tcatcgcctt
     4201 tccgatggtt aatattactg aggccagcca gctgtgggat atcgctcagg cgcgcgtttg
     4261 cgaggtgata gtcggtattt tgtgcggcgg catgatgatg atgatcctgc ctagcagttc
     4321 cgatgctact gcccttttaa ctgcattgaa aaacatgcac gcccgattac tggaacatgc
     4381 cagtttactc tggcagcctg aaacaaacga tgccattcgt gcagcacatg agggggtgat
     4441 tgggcagata ctgaccatga atttgctgcg tatccaggct ttctggagcc actatcgttt
     4501 tcgccagcaa aacgcgcgcc ttaatgcgct actccaccag caattacgta tgaccagtgt
     4561 catctccagc ctgcgacgta tgttgctcaa ctggccctca ccgccaggtg ccacgcgaga
     4621 aattctcgaa caattgctga cggcgctcgc cagttcgcaa acagatgctt acaccgtcgc
     4681 acgtattatc gccccgctac gcccgaccaa cgtcgccgac taccggcacg tcgccttctg
     4741 gcagcgacta cgttattttt gccgccttta tctgcaaagt agtcaggaat tacatcgtct
     4801 gcaaagcggt gtagatgatc atgccagact cgcacggaca tccgctctgg ctcgtcatac
     4861 cgacaacgcc gaagctatgt ggagcgggct gcgtacattt tgtacgttga tgatgattgg
     4921 cgcatggagt attgcatcgc aatgggatgc cggtgccaat gcattaacgc tggcagcaat
     4981 tagctgcgta ctctactccg ccgtcgcagc accgtttaag tcgttgtcac tgctgatgcg
     5041 cacgctggtg ttacttttgg tcaaatttgg cctgatggtc cagattagcg atctgtggca
     5101 atttttactg tttctctttc cactgctggc gacaatgcag cttcttaaat tgcagatgcc
     5161 caaatttgcc gcactgtggg ggcaactgat tgtttttatg ggttcgttta tcgctgtcac
     5221 taatcccccg gtgtatgatt ttgctgattt tcttaactat aatctggcaa aaatcgttgg
     5281 cgttgcgctg gcttggctag cgttcgccat tctgcgtcca ggatcggatg ctcgtaaaag
     5341 ccgccgccat attcgcgcgc tgcgccggga ttttgtcgat cagctaagcc gccatccaac
     5401 actgagtgaa agcgaatttg aatcgctcac ttatcatcac gtcagtcagt tgagtaacag
     5461 ccaggatgcg ctggctcgcc gttggttatt acgctggggt gtagtgctgc tgaactgttc
     5521 tcatgttgtc tggcaattgc gcgactggga atcgcgttcc gatccgttat cgcgagtacg
     5581 ggataactgt atttcactgt tgcggggagt gatgagtgag cgtggcgttc agcaaaaatc
     5641 actggcggcc acacttgaag aattacagcg gatttgcgac agccttgccc gtcatcatca
     5701 acctgccgcc cgtgagctgg cggcaattgt ctggcggctg tactgctcgc tttcgcaact
     5761 tgagcaagca ccaccgcaag gtacgctggc ctcgtaatta cttaattaca ccacaggcat
     5821 agcgttcacc gccaccgccc agcggtttag gttgatcgga catattatcg ccgccaacgt
     5881 ggaccatcag cgccttgtct ttgatttcat ccagtgattt cagacgaggc gcgatgacgg
     5941 catcggtagc tttgccgtca ttattgacga ccagtgcagg cagatcgcct aaatgcccgg
     6001 taccttctgg cccttcatgt ttaccggtat tttgtggatc aagatgcccg cctgcggatt
     6061 ccgcggcgct ggctttgcca tctttggtgg ctggctggca gcttcctttg gcatgaatat
     6121 ggaagccatg ttcaccgggg ggtaatgctt tcagatcggg tgaaaactcc agacctttat
     6181 cggtttcagt aatggtgacg ctaccaattg actgcccaac cccttgcgac gtgacgaggt
     6241 tcatctcgac tttttcactg gcagcttgtg cgccggtcgc aacaaccagc gccagaatgg
     6301 ccagactaaa acgtttcata ggacctccgt tcattgtgca ttcctgaata agtgtaaacc
     6361 agtgacacat ttttgaacgg cggctattcc taaaagtgct tacggtacgt cgtaccccag
     6421 tgcagcttta cggatacgaa accattgttg acgggtcatt ttcagtgttt ctgcttcgac
     6481 agctgcccgt acgcgctcaa ttttacctga gccgataatt ggcagcggct gcgacggtaa
     6541 acgtaatacc caggcgtaaa ccacctgttc aatcgagccc gcgtttaact cctccgccac
     6601 cacagccagt tcatcacgca gcggctggaa ataatcatca ttaaacagac gaccaccacc
     6661 aaggcaggac cacgccatcg gacgaacacg cagttgttgt agttggtcga gcgtgccatc
     6721 cagcagtaac ggctgatgca ccggggatat ttccacctga ttagtggcaa gggtaaacgg
     6781 cagacgtgat tgcaacaggg caaattgcgc aggcgtaaag ttcgatacgc caaaatgacg
     6841 cactttgccg ctctgatgca gatgtttgaa cgcgtccgcc acttcatcgg catccattaa
     6901 cgggtctggt cggtggatta acagcagatc cagatgatcg gtcgcgagat taattagcga
     6961 ctgttcggcg ctcttaatga tgtgattgcg gtcagtgatg taatgaccaa tgacgttttc
     7021 ttcacgcgcg gtcgtcgcga taccgcattt actgacgatt tccatccgtt cacgcaggtg
     7081 aggtgccagt ttcagtgcct cgccaaacgc cgcttcgcac tgatagccac cataaatatc
     7141 agcatggtcc acggtggtca cgccgagatc cagatgctct tcaataaaac tgaccagctg
     7201 gcgggcggac atattccagt ccatcaatcg ccagtagccc atcacaaaac gggaaaactc
     7261 cgggccttcc ggcgcaatag taatacgctg aaccataatc gcttcctctt atcagatata
     7321 agaggagtat acgcaagatt aggttcaaaa gagtgatggt tgctccggtt cgtctgatga
     7381 cgctggctta tttgcgcgta atttgcgcat taatcgctgc cgacaaaggc gcagcacctc
     7441 ttgtttttcg ccatcgctca ttttattcca gttaaaacgc tcatcccgac tacgaaaaca
     7501 gccgcgacaa aacccgcgtt cgtcagactg gcaaattccc cggcacgggc tctggacggg
     7561 aaagaactct aattgctccg ccacctcgcc ctcctcagat aagattatta ccattattga
     7621 agctgttaat gtccaaagta gcaactttgc ttgcactaga ccgactggtc tactacactc
     7681 caacgcatga acaaacacac cgaacatgat actcgcgaac atctcttggc gacgggcgag
     7741 caactttgcc tgcaacgtgg attcaccggg atggggctaa gcgaattact aaaaaccgct
     7801 gaagtgccga aagggtcctt ctatcactac tttcgctcta aagaagcgtt tggcgttgcc
     7861 atgcttgagc gtcattacgc cgcatatcac cagcgactgg ctgagttgct gcaatccggc
     7921 gaaggtaact accgcgaccg catactggct tattaccagc aaacactgaa ccagttttgc
     7981 caacatggaa ccatcagtgg ttgcctgaca gtaaaactct ctgccgaagt gtgcgatctg
     8041 tcagaagata tgcgcagcgc gatggataaa ggtgctcgcg gcgtgatcgc cctgctctcg
     8101 caggctctgg aaaatggccg tgagaaccat tgtttaacct tttgtggcga accgctacaa
     8161 caggcacaag tgctttacgc actatggctt ggcgcgaatc tgcaggccaa aatttcgcgc
     8221 agtttcgagc cactggaaaa cgcgctggcc catgtaaaaa acattattgc gacgcctgcc
     8281 gtttagcagg cattttttat caccagacga ccgggagcct ttatgtcatc tgaaaaactg
     8341 tattccccac tgaaagtggg cgcgatcacg gcggcaaacc gtatttttat ggcaccgctg
     8401 acgcgtctgc gcagtattga accgggtgac attcctaccc cgttgatggc ggaatactat
     8461 cgccaacgtg ccagtgccgg tttgattatt agtgaagcca cacaaatttc tgcccaggca
     8521 aaaggatatg caggtgcgcc tggcatccat agtccggagc aaattgccgc atggaaaaaa
     8581 atcaccgctg gcgttcatgc tgaaaatggt catatggcct tgcagctgtg gcacaccgga
     8641 cgcatttctc acgccagcct gcaacctggc ggtcaggcac cggtagcgcc ttcagcactt
     8701 agcgcgggaa cacgtacttc tctgcgcgat gaaaatggtc aggcgatccg cgttgaaaca
     8761 tccatgccgc gtgcgcttga actggaagag attccgggta tcgtcaatga tttccgtcag
     8821 gccattgcta acgcgcgtga agccgctttt gatctggtag agctccactc tgctcacggt
     8881 tatttgctgc atcagttcct ttccccttct tcaaaccatc gtaccgatca gtacggcggc
     8941 agcgtggaaa atcgcgcacg tttggtactg gaagtggtcg atgccgggat tgaagaatgg
     9001 ggtgccgatc gcattggcat tcgcgtttcg ccaatcggta ctttccagaa cacagataac
     9061 ggcccgaatg aagaagccga tgcactgtat ctgattgaac aactgggtaa acgcggcatt
     9121 gcttatctgc atatgtcaga accagattgg gcggggggtg aaccgtatac tgatgcgttc
     9181 cgcgaaaaag tacgcgcccg tttccacggt ccgattatcg gcgcaggtgc atacacagta
     9241 gaaaaagctg aaacgctgat cggcaaaggg ttaattgatg cggtggcatt tggtcgtgac
     9301 tggattgcga acccggatct ggtcgcccgc ttgcagctca aagctgagct taacccacag
     9361 cgtgccgaaa gtttctacgg tggcggcgcg gaaggctata ccgattaccc gacgttgtaa
     9421 tccaacattg cgagcggcgt aaagccgccg ctatactaaa acaacatttt gaatctgtta
     9481 gccattttga ggataaaaag atgcgtcttc ttcataccat gctgcgcgtt ggcgatttgc
     9541 aacgctccat cgatttttat accaaagtgc tgggcatgaa actgctgcgt accagcgaaa
     9601 acccggaata caaatactca ctggcgtttg ttggctacgg cccggaaacc gaagaagcgg
     9661 tgattgaact gacctacaac tggggcgtgg ataaatacga actcggcact gcttatggtc
     9721 acatcgcgct tagcgtagat aacgccgctg aagcgtgcga aaaaatccgt caaaacgggg
     9781 gtaacgtgac ccgtgaagcg ggtccggtaa aaggcggtac tacggttatc gcgtttgtgg
     9841 aagatccgga cggttacaaa attgagttaa tcgaagagaa agacgccggt cgcggtctgg
     9901 gcaactaatc tcctgccggg cgtgaactca tcgcgcccgc atctttactg catcgacaag
     9961 taatatttgt cataatgcgc gctgcaattt atccgtatta agagaatcag atgtccgata
    10021 acgctcaact taccggtctg tgcgaccgtt ttcgtggttt ttatcctgtt gtgatcgatg
    10081 ttgaaacagc cggatttaac gccaaaaccg atgcgctgct tgagattgcc gccatcaccc
    10141 tgaaaatgga tgaacaaggc tggctgatgc cggacaccac attacatttc cacgtcgaac
    10201 catttgtcgg cgcaaatttg caaccagaag ccctcgcctt caacggcatt gacccgaacg
    10261 atcccgatcg cggcgcggtc agcgaatacg aggcgctgca cgaaattttt aaagttgtac
    10321 gtaaaggtat taaagcgagc ggctgtaacc gcgccattat ggtggcgcac aacgccaatt
    10381 ttgatcacag ctttatgatg gccgccgcag aacgcgcctc gctgaaacgt aacccgttcc
    10441 accctttcgc cacttttgac actgctgcac tggccgggct ggcactcgga caaaccgtat
    10501 tgtcaaaggc ttgccagacc gctggcatgg acttcgacag cacccaggcg cactccgcgc
    10561 tgtacgacac cgaacgcact gctgtgctgt tttgtgaaat cgtcaaccgc tggaaacgtc
    10621 tgggaggctg gccgctacct gccgccgaag aggtgtaatc gagtcgatgc ctgatgacat
    10681 gcaatgattc aggcatctat agtgaggcta ttccacgcat caagcatgat attcacgggg
    10741 aatagcgtta atggcagata atccagaccc ttcatcgctc ctgccggacg tgttttcacc
    10801 ggcgacccgc gactggtttc ttcgcgcctt taaacagccg accgctgtcc agtcgcaaac
    10861 ctggcatgtg gcggcgcgag gcgaacatgc gctggtgatt gcaccgactg gctccgggaa
    10921 aacgctggca gcatttctct acgccctcga tcggctcttc cgcgaaggcg gcgaagatac
    10981 ccgcgaggcg cataagcgta aaacctcacg catcctctat atttcaccga taaaagccct
    11041 gggcaccgac gttcagcgca acttgcagat cccgttgaag ggtattgccg atgaacggcg
    11101 gcggcgcggc gaaacggtaa ttattgatga agtccacgcg gtagcgggca gtaaacgtgg
    11161 tgcgcatctg gcgttaagtc tggagcggct cgatgcgctg ctccacacct cagcacagcg
    11221 aattggcctt tctgccactg tgcgctcagc cagcgatgtg gcagcatttc ttggtggcga
    11281 tcgcccggtt acggtagtca acccgcccgc aatgcgccat ccgcagatac gaattgtcgt
    11341 accggtcgcc aatatggatg atgtctcatc ggtcgccagc ggcaccggcg aagacagcca
    11401 tgccggccgg gaaggctcca tctggccata tattgaaacg ggtatccttg atgaagtgtt
    11461 gcgccatcgc tcgaccattg tctttactaa ttcacgcggg ctggcggaaa aactgacagc
    11521 acgattaaat gagctttacg ccgcacgctt acagcgttcc ccgtctatcg ccgttgatgc
    11581 ggcccatttc gagtcgacct ccggcgcaac ctctaaccgt gtacaaagta gcgacgtttt
    11641 tattgcccgc tcacaccacg gctccgtctc taaagaacaa cgagcaatca ccgaacaggc
    11701 gctgaaatcg ggtgaattac gctgcgtggt cgcaacctcc agtcttgaac tggggattga
    11761 tatgggcgcg gtggatctgg tgattcaggt ggcaacgccg ctttctgttg ccagtgggtt
    11821 acaacgcatt ggtcgcgccg gacatcaggt tggcggtgta tctaaagggc tgtttttccc
    11881 ccgtacccgg cgtgatttag tcgattccgc agtcattgta gagtgtatgt tcgcaggcag
    11941 gctggaaaac ctgacaccac cgcataatcc tctcgacgtc cttgcgcagc aaaccgttgc
    12001 cgccgcggcg atggatgcat tacaggtaga cgaatggtac tcccgcgtac gccgtaccgc
    12061 accgtggaaa gatctgccaa gacgtgtttt tgacgccacg ctggatatgc tttccgggcg
    12121 ctatccctct ggcgattttt ctgcttttcg ccccaaactg gtctggaaca gggagaccgg
    12181 gatattgacc gcccgacctg gcgctcaatt gttggcggtt accagcggcg gcaccattcc
    12241 ggatcgtggc atgtatagcg tgttattacc cgaaggtgaa gaaaaggccg gttcgcggcg
    12301 ggtgggtgaa ctggatgagg agatggtata tgagtcgcgg gtgaacgaca ttatcactct
    12361 cggcgctacc tcatggcgga tccagcaaat cacccgcgat caggtgattg tgactcctgc
    12421 tccgggtcgt tctgcccagc tccccttctg gcgtggtgaa ggtaacggac gtccggctga
    12481 attaggcgag atgatcggcg attttcttca tttactggcg gatggcgcgt tcttttccgg
    12541 gactattccc ccgtggctgg cagaagaaaa tacgaacgcc aatattcagg ggttgattga
    12601 cgagcaacgc aacgcgacgg gcatcgttcc ggggagtcgc catctggtcc tcgaacggtg
    12661 ccgtgatgaa attggcgact ggcgtattat tttgcactct ccctatggaa gacgggtgca
    12721 tgaaccctgg gcgctggcga ttgccgggcg aatacatgcc ctatggggcg ctgacgcgtc
    12781 tgtggtcgcc agtgatgacg gcattgttgc gcgcattcct gacactgatg gtaaattgcc
    12841 cgacgccgcg atttttttgt ttgaaccaga aaagttgctg caaattgtcc gcgaggcggt
    12901 aggcagctcg gcacttttcg ccgcccgttt tcgcgaatgc gccgcgcggg cattattaat
    12961 gccgggccgc acgccgggcc atcgcacccc gctttggcaa caacggctgc gcgccagtca
    13021 gttgctggaa atcgctcagg gatatccgga ttttccggtc attctcgaaa ccctacgcga
    13081 atgtctgcaa gatgtttatg atcttcccgc actggaacgt ttgatgcgtc gcctgaacgg
    13141 tggcgaaatt caaatatccg atgtaacgac cactacgccc tcgcctttcg ccacaagttt
    13201 attgttcggc tatgtcgcgg aatttatgta ccagagcgac gccccgctgg cagagcgccg
    13261 ggcatccgta ctgtcgctgg acagcgagtt actgcgcaat ctactcggac aggtcgatcc
    13321 gggggaatta ctcgacccgc aggtcattcg ccaggtggaa gaagagttgc aacgactggc
    13381 tcctggtaga agagcgaaag gtgaagaagg attgttcgac ctgctgcgcg aactggggcc
    13441 aatgaccgtt gaagacctgg cgcaacggca tacaggcagc agtgaagagg ttgcgtcgta
    13501 tctggaaaat cttcttgcag taaaacgaat cttcccagcg atgattagcg gacaggagcg
    13561 tcttgcctgt atggatgatg ccgccaggct gcgtgatgcc ctcggcgtac ggctaccaga
    13621 gtcattgcca gagatttatt tacatagagt cagttacccg cttcgcgacc tctttctgcg
    13681 ctatctccgg gctcatgctc tggtcacggc tgaacaactg gctcatgagt ttagtctcgg
    13741 tattgccatt gtcgaagagc agcttcagca actgcgtgaa cagggtctgg tgatgaatct
    13801 gcaacaaaac atctgggtga gcgatgaagt atttcgtcgt ctgcgtttgc gctcgctgca
    13861 agccgccaga gaagcgacgc gtcccgttgc agccacgacc tatgcgcgat tgctgctgga
    13921 acgtcagggc gtattacccg ccaccgatgg tagcccggcg ctctttgcct caacatctcc
    13981 aggcgtttat gagggcgtag atggcgtgat gcgggtgatc gaacagcttg ccggagtcgg
    14041 tttacccgcc tcactctggg aaagccagat cctgcctgcc cgcgtacgcg actattcgcc
    14101 agaaatgctc gatgaattac tggcaaccgg agcggttatc tggtcgggac aaaaaaaagc
    14161 tgggtgacga tgacggcctg gtggcactgc atctacagaa atatgccgca gaatcgttca
    14221 ctcccgccga agcggatcag gcgaatcgtt cggcgctgca gcaagcgata atcgctgttc
    14281 tggctgacgg aggagcctgg tttgcacaac aaatcagcca acggatacgc gataaaatcg
    14341 tcgaatcggt tgattcctcg gcactgcaag aggcgttatg ggcgctggtc tggcaaggcg
    14401 tcattaccag cgatatttgg gcaccgttac gcgccctcac ccgcagcagt tccaacgcac
    14461 gcacctcaac tcgccgcagt caccgggctc gtcgtgggcg tcctgtctat gcacaacccg
    14521 tctcgccgcg ggtatcttac aacacaccaa atctggctgg acgctggtcg ttattgcagg
    14581 tggagccact aaacgatacc gaaaggatgc tggcgctggc ggaaaatatg ctcgaccgct
    14641 acggcatcat cagtcgtcag gcggtgatag ccgaaaatat ccctggcggg tttccatcga
    14701 tgcaaacact ttgtcgaagt atggaagact ccgggcgaat tatgcgaggt cgttttgtag
    14761 aaggtctggg tggcgcgcaa ttcgctgaac gtctgactat tgaccgattg cgcgatctgg
    14821 cgacacaagc cacgcaaaca cgccactata caccagtggc gctctccgcc aacgatccgg
    14881 ctaatgtgtg gggaaatctt ctgccctggc ctgcacatcc ggcaacgctg gtaccaacgc
    14941 gtcgggcggg tgcgctggtg gtcgtttctg gcggcaaatt gttactctat ctggcgcaag
    15001 gcggcaaaaa aatgctggtc tggcaggaaa aggaggaatt actcgcccca gagattttcc
    15061 aggcgctgac caccgcactg cgtcgcgaac caaggctgcg ctttacgcta acagaagtga
    15121 atgatcttcc ggtccggcaa acgccgatgt ttacgctgct gcgtgaggcg ggattttcaa
    15181 gttcgccaca agggctggat tggggatagt tgacatcctc cacgccctga aagacgtggt
    15241 tccctgctac gttcaagctg tcgcctgaat catttcggtg ggttcctgct tcaacgagcg
    15301 gcctgactgc accatctctc cacaggcaag aacggcgtgc cccgccgcta aaatgttacg
    15361 agcgccattt acatcggcgt tcgctgtata tccacatacc tggcatctga atttactttg
    15421 tgacaggcgg ttttctttcg ctgtatgacc acagcacgcg caacgctggc ttgtgtacgc
    15481 tggcggaaca gcaagcacct gaccgccact ccagagctgc ttgtactcaa gctggcggcg
    15541 catttcatac cagccctgat ccagtatcga acggtttaaa cctgattttg cccggacatt
    15601 gcgccccggc tggctgaccg tacccgctgc tgactttgac atgttgctga ccttcaaatc
    15661 ctcaatgact atcattgcgt ggtttttgct gacggtcgtt gtgactttgt gaaggtagtc
    15721 cctgcggata tttgcgatac aggaatgcag tcgctgtatt ttgcgtttct gcttctgcca
    15781 gttgttgctg aatttgacct tccggcttaa ctgtcgctga agtctcgcca gcgtcttctg
    15841 gtttttttga aaactgttta caggctcaaa gactgtgcca tctgacagcg tggcgagttt
    15901 agccacgcca gcatccagcc cgatcattga tgctgaaggg tgaaccggag ttgatacttc
    15961 actttctgtc tgaatactga tgtaccacgt accgcaggac tggctgacag tgacattttt
    16021 cacaactccc gtgacctgac ggctattccg gtagcgcatc cagccaagtt tcggcagaaa
    16081 aatacggctg ttttcctgat cgagcttaac gtcctgcggg tagcggaatg cagcattctg
    16141 tccccgcttt ttgaatcggg gaaaagcagc ccgcttctgg aagaagtttt tgtaagcccg
    16201 ctcaaggtct ttcagtgact gttgcaatgg ctgtgagggc gaatctttaa gccattgcgt
    16261 ttcagtggcg tttttccact caaccagcca ggaagccatt ttcccgtaag ggatgtattt
    16321 attaccggcc tcatgattct cattctgaag cgccagagca cgattgaaaa cgaaacgaca
    16381 tgcgcctgcg aagcgcctca tttcacgctc ctgttgacca cctggtctta actggaattt
    16441 aaatgcctgt aatcgtttca caggtcaaat gatactttgg tctgtgaaaa aagaaaccga
    16501 tatccggcgt ggaaggcatt gtgttttcct gatgcatgtt cacctggtct ttgtcaccaa
    16561 ataccgacgc cagatttttg accatgacac gacagaaaaa ctacgcactt acttttcaaa
    16621 tgtatgtgct gattttgcag ctgaactggt tgaaatggat ggcgaaccag atcacgtcca
    16681 tttgttaatt aactatcctc ccaaactggc gatatccagt ctggtaaaca gcctcaaagg
    16741 catatcgaat aggttactgc gacgagatcg accagatatt gcagtcaggt attactacaa
    16801 aggcgttttg tggagtcctg gctattttgc cagtagctgc gaaggtgcgc caatatccgt
    16861 catccgccag tacattgaac aacagcaaac acctggtcag gtggaaaacc gcgccttata
    16921 tccccgccct gaaggacgag gttttacgac gcaccggata aaggactgac ggatgcccgt
    16981 tcgcatccgt cagtattgca ggacggatta ttccgcgtcc ggctcttcag acttgtattt
    17041 agcggcagtt tctttgatca gctgctgcag ttcgccacgc tgatacattt cgatcacgat
    17101 atcacaaccg ccgaccagct cgccgtcaac ccacagttgc gggaaggtcg gccagttagc
    17161 atatttcggc agttccgcac gaatgtccgg attctgcaga atatcaacat aggcaaaacg
    17221 ttcgccacat gcggcaagcg cctggactgc ctgggcagag aaaccgcagc tcggcagttt
    17281 cggtgaacct ttcatgtaca gcaggatcgg gttttcagca atctggcgtt ggattttttc
    17341 gatagtggtg ctcattgtct tgcttcctca aacttcttta cggcagtaat ctgacattgt
    17401 agcgggtcag tgcggcatcg gaaaataaca ttttcatcac gcttttgcta ttttatccct
    17461 ttgctctatt tgttgcattt caaatattcg tttcttctct gatgcgcggc aaactggcgt
    17521 atttataacc atttttagct ttcactgctg catttttttg cgctcgccaa cgaaacgtat
    17581 tttttaacaa taaaagctat taactttctc ttcttctatg cattagaatc atcaagtttt
    17641 gtaaatcaga cgcaggcatg atagacctgc cttaacagag ggacgctcag tggcgcggat
    17701 aaaccgtatt tcgatcacgc tctgtgcttt actttttacc accctgcctt taacgcctat
    17761 ggcccatgct tcaaagcaag ccagggagag ttctgctacc actcatatca ccaaaaaagc
    17821 agataaaaag aaaagcacgg caaccaccaa aaagacccag aaagccagcc agaaaacagc
    17881 gaaaaaagcc gccagtaaaa gtatgaccaa aagcaaaacc gcttcttctg ttaaaaaatc
    17941 ttccattacc gcttctaaaa acgccaaaac tcgcagcaaa cacgccgtca ataaaacggc
    18001 ctcagccagc tttaccgaaa agtgtaccaa acgtaagggc tataaatcgc attgtgtgaa
    18061 agtcaaaaat gccgcgtcag gaacccttgc cgacgcgcac aaagcgaagg tgcaaaaagc
    18121 cacgaaagtg gcaatgaata aactgatgca gcaaattggt aagccatatc gttggggcgg
    18181 cagctcaccg cataccggtt ttgattgcag cggcctggtt tattacgctt ataaagattt
    18241 ggtgaaaatt cgtattccgc gtacggcgaa tgaaatgtat cacctgcgtg atgcaggacc
    18301 tatcgaacgt agtgagctga aaaacggcga cctggtcttt ttccgtactc agggacgcgg
    18361 tacagccgat catgtcggcg tgtatgtcgg caacggcaaa tttattcagt caccgcgcac
    18421 aggtcaggaa attcaaatca cttctctcag tgaagactac tggcagcgcc actatgttgg
    18481 cgctcgtcgg gtaatgaccc caaaaacact tcgctaaaac tttaccctgt tgttacggca
    18541 acagggtaag ttcatctttt gtctcacctt ttaatttgct accctatcca tacgcacaat
    18601 aaggctattg tacgtatgca aattaataat aaaggagagt agcaatgtca ttcgaattac
    18661 ctgcactacc atatgctaaa gatgctctgg caccgcacat ttctgcggaa actatcgagt
    18721 atcactacgg caagcatcat cagacttatg tcactaacct gaacaacctg attaaaggta
    18781 ccgcgtttga aggtaaatca ctggaagaga ttattcgcag ctctgaaggt ggcgtattca
    18841 acaacgcagc tcaggtctgg aaccatactt tctactggaa ctgcctggca ccgaacgccg
    18901 gtggcgaacc gactggaaaa gtcgctgaag ctatcgccgc atcttttggc agctttgccg
    18961 atttcaaagc gcagtttact gatgcagcga tcaaaaactt tggttctggc tggacctggc
    19021 tggtgaaaaa cagcgatggc aaactggcta tcgtttcaac ctctaacgcg ggtactccgc
    19081 tgaccaccga tgcgactccg ctgctgaccg ttgatgtctg ggaacacgct tattacatcg
    19141 actatcgcaa tgcacgtcct ggctatctgg agcacttctg ggcgctggtg aactgggaat
    19201 tcgtagcgaa aaatctcgct gcataataac tgatggcaaa tgcagcattg cctgatgcgc
    19261 ttcgcttacc atgccaacgc ggatgaggcg ttaacagcgc attcggcaag ataaatcgca
    19321 cgttgtcagc aactgtaacg cagaaggtta tccttctgcg tttttgttta attagctgtt
    19381 agcaacgcaa actgtttcag gttgttttct ggctgacata aacaccagca ataatgccag
    19441 tcccgcgaca atcgcccccg tcaccggcac aaagctgtat cccagcccag cggaaattac
    19501 cgcaccacca gcagctgctc ccagcgcatt tccaagatta aaggcaccaa tattgactga
    19561 tgaagacaga cctggcgctt cactggcgac atgcatcacg cgcatctgta acggaggtac
    19621 aaccgcaaag gttgctgcgc cccacaccac catgctaata gctgcgccga actcattgcg
    19681 ggccaggaac gggattgcca gcataatcac catcagcagc aacaaaaagc ctttcaacgt
    19741 gccgttaact gaacgatctg ccagtttgcc gccgagatag ttaccgatag agaatccgac
    19801 accaatcagc accagcattg ccgtgacgaa caccggtgtt gcgtgggtaa tactttgcag
    19861 taccggagag atataggtgt agagggtaaa cattgcacca gctcccagta ccgtcgtcag
    19921 caatgcagac agcacctgcg gacgcattaa taccgccagc tcttttttca cttcaggtcg
    19981 tgcccctgta ccacctttag gtaatgagaa gaacagactt accattgaaa tcactcccag
    20041 ccccgccgtt gccagaaatg acatccgcca gccgatggtt tcacccaacc aggtcgccgc
    20101 cggcacgcca ccgatatttg ccagggttaa ccccataaac atagtggcaa ctgcgctggc
    20161 ctgtttatgt tttggcacca cgcttgcggc cacgactgaa cccaaaccaa aaaatgctcc
    20221 gtgattcagg ctggtcaaaa tacgtgaaag catcagggtc atataatccg gcgcgatggc
    20281 ggaaagaacg ttgcctagcg tgaaaattgc catcaggaaa atcaacgcac tgcggcgggc
    20341 acgatgagaa agtagaagcg tcatcagcgg cgcgcccacc attacgccaa ctgcataggc
    20401 actgattaac attccggcag cgggaatcga gacatccaca ccgcgcgcaa tgacgggcaa
    20461 caagcccatt ggcgagaact ccgttgtccc gataccaaac gcgccaatcg ccagcgccag
    20521 caacggatag ttaattttca tgccttatct ccacctcttc gcgtcattac gcgatattca
    20581 ttaaagtggc gaaagcatga cagcaatcac aaaaaaatga aaataacaaa aagagaaaac
    20641 acttttgcca ttttgctaac aaacaggaag gagatgcgag ggagaacgcg ctccctcgag
    20701 aggaaatcag tgcagcgcgg cagtcaaacc cacggctacg atcaaaccga ggacgataat
    20761 cgttgttacc agtgaaaatt taaggtcggt gctcatcaag ttttctcctt ttttattacc
    20821 acacaaaaag tgatattacg catttttaca cactgtgatg aaaaaatctc ccgtcattta
    20881 taatgataag tgtttttacc acttcccctt ttcgtcaaga tcggccaaaa ttccacgctt
    20941 acactatttg cgtactggcc attgacccct tcctgacgct ccgtgtcgtt tttccggcgt
    21001 accgcaacac ttttgttgtg cgtaaggtgt gtaaaggcaa acgtttacct tgcgattttg
    21061 caggagctga agttagggtc tggagtgaaa tggaatggca acaataaaag atgtagcgaa
    21121 acgagcaaac gtttccacta caactgtgtc acacgtgatc aacaaaacac gtttcgtcgc
    21181 tgaagaaacg cgcaacgccg tgtgggcagc gattaaagaa ttacactact cccctagcgc
    21241 ggtggcgcgt agcctgaagg ttaaccacac caagtctatc ggtttgctgg cgaccagcag
    21301 cgaagcggcc tattttgccg agatcattga agcagttgaa aaaaattgct tccagaaagg
    21361 ttacaccctg attctgggta atgcgtggaa caatcttgag aaacagcggg cttatctgtc
    21421 gatgatggcg caaaaacgcg tcgatggtct gctggtgatg tgttctgagt acccagagcc
    21481 gttgctggcg atgctggaag agtatcgcca tatcccaatg gtggtgatgg actggggtga
    21541 agcaaaagct gacttcaccg atgcggtcat tgataacgcg ttcgaaggcg gctacatggc
    21601 cgggcgttat ctgattgaac gcggtcaccg cgaaatcggc gtcatccccg gcccgctgga
    21661 acgtaacacc ggcgcaggcc gccttgccgg ttttatgaag gcgatggaag aagcgatgat
    21721 caaggtgccg gaaagctgga ttgtgcaggg tgactttgaa cctgaatccg gttatcgcgc
    21781 catgcagcaa atcctgtcgc agccgcatcg ccctactgcc gtcttctgtg gtggcgatat
    21841 catggcaatg ggcgcacttt gtgctgctga tgaaatgggc ctgcgcgtcc cgcaggatgt
    21901 ttcgctgatc ggttatgata acgtgcgcaa cgcgcgctat tttacgccgg cgctgaccac
    21961 gatccatcag ccaaaagatt cgctgggtga aacagcgttc aacatgctgt tggatcgtat
    22021 cgtcaacaaa cgtgaagaac cgcagtccat tgaagtgcat ccgcgtttga ttgaacgccg
    22081 ctccgtggct gacggcccgt tccgcgacta tcgtcgttaa tcacccgttg cgggagtctc
    22141 ttccggctcc cgtagccact ccttattcag cgtctcacta tcgccgagat actcaagcag
    22201 ccaggttaac gcaggcgaca tatcattttg ctgccatgtc agacaacatg ccgaatccgg
    22261 gaaggggttt tccagttcaa gtgctaccca cttcccctca ttaagccacg gtttggcgaa
    22321 atgtgttggc accatcccta tgcataatcc tgccgagata caggttgccg atgattccca
    22381 gtcaggtacg acaagtcttt tttggttatc cagcaaccag gtaatacgtt taggtagcgt
    22441 tcgcgaggtg tcttcgcgca ccaacgacgg ccagttgcgc aacgtatcat cgctgaacgg
    22501 gccatccatc aacgccagcg ggtggtgact ggcaacaacg caactccagc ttagcatccc
    22561 catatcccgg aaggcataac gaccgcctac cggaatcgcg cgtgttgcgc caattgccag
    22621 ttccacgcgc ccgtcggaaa gcgcatccca gacaccgttg aacacttcct gaaagacaag
    22681 aagttcgaca tcatcaaaat ggcgataaaa atcaacgatc atctgccgtg tacgttctgg
    22741 cctgacaata ttatccactg cgatagctaa ctgaccgcgc cagccgttcg ctatctgctg
    22801 acattgctgg cgggtgatct gcattttttt gacaacagag cgcccttctt tgagaaacca
    22861 cgctccagca gcggtcagct ccacatcacg gtgccgtcgt tcaaagagcg gtaccgccag
    22921 ccactcttcc agctgacgca cggtatagct gaccgcagaa ggaacacgat gcagctcctg
    22981 tgccgcagcg ctaaaactac cattacgcgc taccgcatca acaacttcga gtgaatattc
    23041 tgaccacata gtctgcctgc aaaatttttg aaaccagtca tcaaatatta ccgtttcaca
    23101 acactaattt cactccctac actttgcggc ggtgtttaat tgagagattt agagaatata
    23161 catgcaacct gggaaaagat ttttagtctg gctggcgggt ttgagcgtac tcggttttct
    23221 ggcaaccgat atgtatctgc ctgctttcgc cgccatacag gccgacctgc aaacgcctgc
    23281 gtctgctgtc agtgccagcc ttagtctgtt ccttgccggt tttgccgcag cccagcttct
    23341 gtgggggcca ctctccgacc gttatggtcg taaaccggtg ttattaatcg gcctgacaat
    23401 ttttgcgtta ggtagtctgg ggatgctgtg ggtagaaaac gccgccacgc tgctggtatt
    23461 gcgttttgta caggctgtgg gggtctgcgc cgcggcggtt atctggcaag cattagtgac
    23521 agattattat ccttcacaga aagtcaaccg tatttttgcg accatcatgc cgctggtggg
    23581 tctgtctccg gcactggctc ccctgttagg aagctggctg ctggtccatt tttcctggca
    23641 ggcgattttc gccaccctgt ttgccattac cgtggtgctg attctgccta ttttctggct
    23701 caaacccacg acgaaggccc gtaacaatag tcaggatggt ctgaccttta ccgacctgct
    23761 acgttctaaa acctatcgcg gcaacgtgct gatatacgca gcctgttcag ccagtttttt
    23821 tgcatggctg accggttcac cgttcatcct tagtgaaatg ggctacagcc cggcagttat
    23881 tggtttaagt tatgtcccgc aaactatcgc gtttctgatt ggtggttatg gctgtcgcgc
    23941 cgcactgcag aaatggcaag gcaagcagtt attaccgtgg ttgctggtgc tgtttgctgt
    24001 cagcgtcatt gcgacctggg ctgcgggctt cattagccat gtgtcgctgg tcgaaatcct
    24061 gatcccattc tgtgtgatgg cgattgccaa tggcgcgatc taccctattg ttgtcgccca
    24121 ggcgctgcgt cccttcccac atgcaaccgg tcgcgccgca gcgttgcaga acactctgca
    24181 actgggtctg tgcttcctcg caagtctggt agtttcctgg cttattagta tcagcacgcc
    24241 attgctcacc accaccagcg tgatgttatc aacagtagtg ctggtcgcgc tgggttacat
    24301 gatgcaacgt tgtgaagaag ttgactgccc gaatcatggc aatgccgaag tcgctcatag
    24361 cgaatcacac tgatctatat cgatatactt atacttaggc tgctaacaaa attttgttgt
    24421 atgattgaaa ttagcggcct atactaattt cgagttgtta aagctacgat aaatattatg
    24481 tttttacggg gacaggatcg ttcccgactc actatggatg gtcatttcgg caagggttcc
    24541 tcctttccct ctgttctacg tcggattata gactcgcggt tttttctgcg agatttctca
    24601 caaagcccaa aaagcgtcta cgctgtttta aggttctgat caccgaccag tgatggagaa
    24661 actatgagtt catcgtgtat agaagaagtc agtgtaccgg atgacaactg gtaccgtatc
    24721 gccaacgaat tacttagccg tgccggtata gccattaacg gttctgcccc ggcggatatt
    24781 cgtgtgaaaa accccgattt ttttaaacgc gttctgcagg aaggctcttt ggggttaggc
    24841 gaaagttata tggatggctg gtgggaatgt gaccgactgg atatgttttt tagcaaagtc
    24901 ttacgcgcag gtctcgagaa ccaactcccc catcatttca aagacacgct gcgtattgcc
    24961 ggcgctcgtc tcttcaatct gcagagtaaa aaacgtgcct ggatagtcgg caaggagcat
    25021 tacgatttgg gtaatgactt gttcagccgc atgcttgatc ccttcatgca atattcctgc
    25081 gcttactgga aagatgccga taatctggaa tctgcccagc aggcgaagct caaaatgatt
    25141 tgtgaaaaat tgcagttaaa accagggatg cgcgtactgg atattggctg cggctggggc
    25201 ggactagcgc actacatggc atctaactat gacgtaagcg tggtcggcgt caccatttct
    25261 gccgaacagc aaaaaatggc tcaggaacgc tgtaaaggcc tggatgtcac cattttgctg
    25321 caagattatc gtgacctgaa cgaccagttt gatcgtattg tttctgtggg gatgttcgag
    25381 cacgtcggac cgaaaaatta cgatacctat tttgcggtgg tggatcgtaa tttgaaaccg
    25441 gaaggtatat tcctgctcca tactatcggt tcgaaaaaaa ccgatctgaa tgttgatccc
    25501 tggattaata aatatatttt tccgaacggt tgcctgccct ctgtacgcca gattgctcaa
    25561 tccagcgaac cccactttgt gatggaagac tggcataact tcggtgctga ttacgatact
    25621 acgttgatgg cgtggtatga acgattcctc gccgcatggc cagaaattgc ggataactac
    25681 agtgaacgct ttaaacggat gtttacctat tatctgaatg cctgcgcagg tgctttccgc
    25741 gcccgtgata ttcagctctg gcaggtggtg ttctcacgcg gtgttgaaaa cggccttcga
    25801 gtcgctcgct aaaggctatt ctatcgcccc ctcttcgggg gcgagttcag atcaggcttc
    25861 tgtacctggt tgattcatgg cattttctcg tgccgccagc acacgttcta ccgtatctac
    25921 cactgcctga gtttgtggat cgatttcaat gttgacgcgt gcgccaagtt ttttcttccc
    25981 aagagtcgtg cgttccagtg tttccggaat taaatggacg caaaaacgcg ttggcgtgac
    26041 ttcgccgacg gtcaggctaa taccgtcgat gccaataaat cctttgtaca ggatatattt
    26101 catcaactga ctatcctgga ctttaaacca gatctggcga ttattttccg aggttaatat
    26161 tttcgccact tcagcggtgg tcataatatg acctgacatt aagtgtccgc caatttcatc
    26221 actgaatttc gccgcacgct caacgtttac ccaatccccc acttttaaat cgccaagatt
    26281 ggtaatgcgt aacgtttctt tcatcaggtc aaaactgaca tggttgccgt taatttccgt
    26341 cacggtcagg cagcaaccgt tatgcgccac ggaagcaccg gtttccaggc cgtccagcat
    26401 gtggtcgggt aactccacca catgcgtacg aaaatttggt ttctcgtcaa tcgacaccag
    26461 ttttgcggtg ccctgtacaa tccccgtaaa catacttaca actcctgaaa tcagttaaga
    26521 cattctgttc agcacaatag caggtggaaa acgcccttac cagtgaaggg gtaagaatgg
    26581 ctattttttc actggagaat taataaatcc tcgctacaat agactgaatt tcccctgctt
    26641 cttctttttg ctgcccattc aggcggcttt ttagtctctc atataactac aaataaaagg
    26701 tgttcacgtg cagaagtata tcagtgaagc gcgtctgtta ttagcattag caatcccggt
    26761 gattctcgcg caaatcgccc aaactgcgat gggttttgtc gataccgtga tggcgggcgg
    26821 ctatagtgcc accgacatgg cggcggtcgc tatcggtact tctatctggc ttccggcgat
    26881 cctctttggt cacggactgc tgctggcatt aacgccggtt atcgcgcaat taaatggttc
    26941 cggtcgacgt gagcgcattg cgcatcaggt gcgacaaggt ttctggctgg caggttttgt
    27001 ttccgttctc attatgctgg tgctgtggaa tgcaggttac attatccgct acatggaaaa
    27061 catcgatccg gctctggcgg acaaagccgt gggttatctg cgtgcgttgt tgtggggcgc
    27121 gccgggatat ctgttcttcc aggttgcccg taaccaatgt gaaggtctgg caaaaaccaa
    27181 gccgggtatg gtaatgggct ttatcggcct gctggtgaac atcccggtga actatatctt
    27241 tatttatggt catttcggta tgcctgagct cggtggcgtt ggttgtggcg tggctactgc
    27301 ggcggtgtat tgggtcatgt tccttgccat ggtttcttac attaaacgcg cccgctccat
    27361 gcgcgatatt cgtaacgaaa aaggcaccgc taagcccgag cctgcggtta tgaaacgact
    27421 gattcaactc ggtttgccga ttgcgctggc actgttcttt gaagtgacac tgtttgccgt
    27481 cgtggctctg ttagtgtctc cgctcggtat tgttgatgtc gcaggacacc agattgccct
    27541 gaactttagt tcactgatgt ttgtgcttcc aatgtcgctg gcggcagcgg taactatccg
    27601 cgtaggttat cgtctgggtc agggctcaac gctggatgcg caaaccgctg cgcggaccgg
    27661 gcttatggtg ggtgtctgta tggcaaccct gacggccatt ttcacggttt cactgcggga
    27721 gcaaatcgcc ctgttgtaca acgacaatcc cgaggttgta acgctggctg cgcatttgat
    27781 gttgctggcg gcggtatatc agatttctga ctcaatccag gtgattggca gtgggatttt
    27841 gcgtggttat aaagatacgc gttccatttt ctatatcacc tttacagctt actgggtgct
    27901 gggcttgcca agcggctata ttctggcact gaccgatctg gtcgttgaac ctatggggcc
    27961 agcaggcttc tggataggct ttattatcgg cctgacatcg gcagccatta tgatgatgct
    28021 gcgtatgcgg ttcctgcaac gtatgccgtc agccatcatt ctgcaacgag cgtcccgcta
    28081 ataaagacaa ggcgcaacct tcacgggttg cgcctgtgtt tttacgcagg ctggagcgtt
    28141 gcgccaatcc cgtcttcgtc tggctgtaat ttcagagcgt tacacagagt ttgccgactg
    28201 gcatctgtta tctcaacgcg tatatccccg ttaacttccc ctttcacatt gatgaagtca
    28261 ggtgaaattt ctttcgcact ggtttgcagg caaatcgctc cctctccgcg caggcgtttc
    28321 actttcagtt gcacatgcgt tgccgcacca gtgatcagcc acagttttcc gccattcaga
    28381 ttgatgttac caacaatgaa agtgacgctg tcacctttta ccgtactgcc gtcgagaatc
    28441 aacacaccgc cctgctgtac agtaatcgtt gattgttgca gctctgcgcc ataaccaata
    28501 atcattgaag cgccgtccag taatgcaaca ctcccggtca gttcaaccgg tgcgcgatct
    28561 tcctctacga caaaattttc gtccgcgctg gtgggtgaca acaggatcac tgttccacct
    28621 tgtattaccg cattttccag cgtaccggca tgcacttcag cccgcccccc cacattcacc
    28681 gacaggtttt gcgtcttacc ggaactgtag agctgaaggc catccgtccc cagacgataa
    28741 atggatccat tctcaacaat agtgtcagag tcttcaccac cgagattatt catggtgcca
    28801 ccagcaagca acgtatcact ggctttcccg gccagcataa aaacgccgcc ctgattaatc
    28861 tcacaattgc tggcttctcc acttggatac accaacaatt caccaccttc atctaccacg
    28921 accgctgagg ttgtcccatt aaccaacagg ccgccctctt gatcgagaat gattttttcc
    28981 gcgcgatgtc cttccagtac acgcaggtta ccgccatttt ccagcaacaa accgcaggca
    29041 taaccctgat caacgctgaa ttcaccttcg ggatggcggc cattaaccgt agcgagcgta
    29101 gagagactaa ttgcgcctcc ctcggactga acaatatctg tggcaatggc accgtcgctg
    29161 accgatagcg tgccattgtt accgtactgt gtgcatttgg cgatcccacc ggttgccact
    29221 ttcaaccagc cagcccccgt gaccacggta ttttcggctc ttccgccagc ttcaacaatc
    29281 aactcgcctt cagtaagttg cgtcgcgcca aggacctcgc cggtcttaac aatttgcaca
    29341 ttcccgtcgc tcatcaagtt tttcgcatca gatcccatga tttattcctt tgctgcatct
    29401 gtgtgccttt attgctacct aagtgtaaag gctacggagg atttatccac gacagatttg
    29461 agatggtggc aaacaactct gtttaaactc tgatacacga attattgggt tgtatcagat
    29521 gtaaatgcga tcctgaataa aaatcgccct tgcaaatcaa caaaatatag ccaatttgct
    29581 taaaagctct gcaaacggtc gaaatagcgc agaaaattac gttttgcctc ttgccacctt
    29641 tccactctgc cgctaatatt cgtccccgtt gtcacctaca acgttgcgtt catagctcag
    29701 ttggttagag caccaccttg acatggtggg ggtcgttggt tcgagtccaa ttgaacgcac
    29761 catcctgcgt ccgtagctca gttggttaga gcaccacctt gacatggtgg gggtcggtgg
    29821 ttcgagtcca ctcggacgca ccagattttc ttaatctggt cttttccttt ttccctctgt
    29881 ttcttctctg tatccaatac gttaaaagat ttacactgtc ttcgtatgcg ttatcagaag
    29941 gagaatcgct atggcaactt tgttacaact tcattttgct tttaatggcc catttggcga
    30001 cgcaatggct gagcagctta aaccacttgc tgagtcgatt aatcaggaac ctggttttct
    30061 gtggaaggta tggacagaaa gtgaaaagaa ccacgaagcc ggtgggatct accttttcac
    30121 tgatgaaaaa agcgcccttg cctatctgga aaaacatact gcccgactga aaaatctcgg
    30181 cgttgaggaa gttgtcgcca aagttttcga tgtcaatgag ccacttagtc aaatcaatca
    30241 ggcaaaactc gcctgacaga atttaatcaa gggcggttag cgcccttttc atccctgtct
    30301 gaaatttctc aaattctaaa aatctcaacc aaacttatct gataacacta aattcgaaag
    30361 aatgcgtaca ggtaagtaac aatgaaaaaa attgctattg tgggtgccgg gcctacgggg
    30421 atctacacct tattctcgct tctacagcaa caaactccac tttctatttc tatcttcgag
    30481 caggctgacg aggccggtgt cgggatgcca tacagtgatg aggaaaactc aaaaatgatg
    30541 ctggcaaata ttgccagtat tgaaataccg ccgattaatt gtacgtatct cgaatggcta
    30601 caaaagcaag aagccagcca tctccagcgt tatggcgtca aaaaagaaac cttgcacgat
    30661 cgtcagtttt taccgcgaat tctgctgggc gaatatttcc gcgatcaatt tttacgatta
    30721 gtagaccagg cacgacagca aaaatttgca gtggctgttt atgaatcatg ccaggttacc
    30781 gatctgcaaa ttacaaatgc tggcgtcatg ctcgctacaa atcaggattt acccagcgag
    30841 acgtttgatt tagcggtgat tgccacgggt cacgtctggc ctgatgaaga agaagcaacc
    30901 agaacgtatt ttcccagccc gtggtcaggc ctgatggaag caaaggtcga tgcgtgtaac
    30961 gtgggtatta tgggaacatc cttgagcgga ctggatgcgg caatggcagt ggctattcag
    31021 catggttcgt tcattgaaga tgataaacaa cacgtcgttt ttcaccgcga taacgcaagt
    31081 gaaaagctaa atattacgtt gatgtcgcgc acgggtattt tacccgaagc cgatttctat
    31141 tgccctattc cctacgagcc cttacacatc gtcaccgatc aggcattaaa tgctgagatt
    31201 caaaaaggtg aagagggcct tttggatcgg gtatttagat tgatagtaga ggaaatcaag
    31261 tttgctgatc cagactggag ccaacgcata gccttagaga gcctgaatgt cgattccttt
    31321 gctcaagcct ggtttgccga gcgcaaacaa cgcgacccat ttgactgggc agaaaaaaat
    31381 ctccaggaag tcgaacgcaa taaacgagaa aaacatactg ttccctggcg ttatgtcatt
    31441 ctgcgcctgc atgaagccgt acaggaaatt gttcctcatc tgaatgaaca cgaccataaa
    31501 cggttcagta aaggccttgc cagggttttc atcgataatt atgcggcaat cccttcagag
    31561 tctattcgtc gactactggc cttacgtgaa gcggggatca ttcatattct cgccctcggt
    31621 gaagactacg aaatggaaat taatgagtcg cgcaccgtcc tgaaaacgga agacaacagc
    31681 tactcgtttg acgtttttat tgatgcccgc ggacagcgtc cgcttaaagt gaaagatatt
    31741 cctttccctg ggctacgcga gcaattacag aaaacagggg atgaaatccc tgatgttggc
    31801 gaagattata cgttacagca acccgaagat attcgtggac gcgtagcgtt cggcgcgttg
    31861 ccctggttga tgcacgacca gcctttcgtt cagggactta cggcatgtgc agaaattggt
    31921 gaggcgatgg ctcgggcggt cgtaaagcct gcatcccgtg ctcgtcggcg tctttcgttt
    31981 gattaaaggt aaagctatca gcactcaaca aactgatgct gttcattaag acgatagcgg
    32041 actccggttc gaatattgtt ttcaccttca atggcgacgg cgaagcgaac tctttcgccg
    32101 tcatgatatg ccagtgcggc gcttccccct agcccgagaa taattgagtc gaccacgccc
    32161 gtgctcacta ccgttgaatt ttcaccgctg gcaatgatgt gtacattgtc gccgctgttg
    32221 gcaattctgg cgtttgcgcc aaagctggcg atttgcacca ggtcaccatt actggcgaca
    32281 tgacaccgtt cacccaatgt acaaacgcgt actcgcattc cggtattcgc aatccgacta
    32341 ctatctccag cgctgcttat ccgcgaagaa ttaccggtta cagcaacccg tgcgcgttca
    32401 ccggagctgc caatgtgact gttaaagccg acacttccaa tccgcacgct ataacccata
    32461 ctggcaattt gcgcggcata cccagcggtt gcgattcggg cgttatcctc acaacagccg
    32521 atgcgagcat gatcgtcgct gcgggcaaac tgactgcaaa atggtatctc tccggggtct
    32581 gtggccagtt tttgcataga ggaaacttcc tgatgcgcga agttttcatc cgccagccat
    32641 tttttccacg catactcaac cagactctcc gcccagtccg tatatccttc ctcaatcagt
    32701 gcatgacgaa tatcggcata actaccgccg cgtgggaaat ggcgcaggaa ccagcgatac
    32761 atcaccgctc cgatacgcca ttctcgcagg tctgctcgtg taatgatcat tcgttagtgc
    32821 cggtgatagc catcgaccat gcttttaaag gtttcgtgtg gcgtacgccc cgtggtgcaa
    32881 agataaagat gaccgaagat aaaaaagagg cttataaatg ccagagcaaa atgcgcctgc
    32941 aataaccagt atcttacgcc aggaaacaca tctcccacgg cttgcggata gagacacaac
    33001 agccccgtca atagtaacaa cggcagcaat ccatacatga caccaacata ggcgacctgc
    33061 tgtaagggat taaatttaga ctgtgttgtt gccgggaaag gatgttcttc cccctgcata
    33121 atgccaaaca aataaaatcg cgtttgtttt gccgctcgtt ccagccaccc ctgacgacga
    33181 atgcgatagt ggtgaccatt atccccaacg gcattgatca gcacaaagcc gagccagcat
    33241 gccagtaaca aaaatccgca aacttcatgc accgcaacca gacttttaac cgcagttgcg
    33301 cccaccatcg caaagtgatt tatcagccca cttgccagca acaatacaaa gagtaacgca
    33361 ttcgaccagt gccataatct gaccgctttg ctgtaaagat aaaccttctc accatgatcg
    33421 gtggctgatt tcttcacgcc acgagcacga agcaaggcgt gcagccccag caccaaccac
    33481 atcccaacca gcaatactcc ggcaatgatc aaccacaccg gccaaaattc gggagtgaac
    33541 tgtggcacat agttcgctaa ctggctctga aactgttcag catgttgcga cgggttcata
    33601 cattttcctt tttaatcaaa tgttgaccga accggcgata cagatgcggt tttccggcac
    33661 caggtagctg atattgatag tatttattat cctgcaacca cgcctgaatt tctgggctat
    33721 cttcgcgacc aaagatcaat gcgtgttccg gacaggcgct gacgcaaatg ggcggaaagc
    33781 cttttgccag tcgggactca gcgcagaaat cacatttatc tgccacttta gtgacagggt
    33841 taaggtagcg aacctggtac ggacaagcgc caatgcaata actgcagccg atgcactgcg
    33901 atttttctac ccgcacaatc ccctgctcat cacgccacga tgcccccgtc gggcatacgt
    33961 cgatgcacgg agcatcttcg cagtgctgac atgactggcg aaaaaagtga tactgcgttt
    34021 cgttgtcgtt atcggtgact ggaatgtgtg ctatcgataa acggcttccc tgagcgggga
    34081 cgtggttagt tttacggcat gcgcgggcgc aaatattaca gccattgcac cgtgactcgt
    34141 catgaatcat ggcataccga acttcctttt cttgcctcgt gttcgctaac agagaagacg
    34201 cagaaccagt aaaaaagatt actgttccca tccccagaac aaatttgcgt cgagtaaagg
    34261 acatctgatt atcccctaat gcagtggctg caactgtcca ccctatccat gacatctccc
    34321 tgaaatttca ggggttctga aactgctcta cctcgaacca ttcgcataag ccataagcct
    34381 tgccataggt cataaatcgc tcgctaaaca agtacaacac cgccgtcgat gactccacct
    34441 gccgtatatc ggcatattca ggtgcaacat ccatcgcggc gatcgcggct tcaatctgcg
    34501 cttcctggaa gtaatatggc gcttgcatca gcatggcgac tttgtaagga cgaggatacg
    34561 tctggcactc aaaacgaacg gcgtgtgcaa tggcgcgaca aatgtcctgc tccacaactt
    34621 gcaaagacat cgccgcgtag ttttcactca tggcctgcgt tgagtaatag taatcatcct
    34681 gacttcccgt catacaagcg atatccgcgt agtctccgtt ctgcttaatg ctttccagca
    34741 aaactgacag ttcctcttct gcaaccgaat agggtggcga gagaaacacg gtgcgcgcca
    34801 ccagctgccc agccgcagaa tgctggcgaa tgaactcagc caggatttca gcaggttctg
    34861 gttcggttac cgtatcaacg gggtgacgca tcccctgcaa ccattgccgc ttcgcctcgt
    34921 caacttcgct gactttcgct aacggcaatt catcctgatg gttcatctta cccattcctt
    34981 acgcaggcag tagattgtgt gctgccagat cggcggcaat atcttccagc cccagacgct
    35041 gcaatgtttc gcgggttgga caaccaagct gtgggtccca gcccatttct ttgtagaaca
    35101 tcgtgagcga agcatgcata tcgtcacgat ccattttgtc agtaccttca gtaaagaccg
    35161 ggatctgcgg atccttgtcg aaaacccagg aacagataag atcgtgttca ttacgcatat
    35221 ctttggtttg catcggtttt accgtgtagg cacgatgcaa cgtaaagata cgctctgcag
    35281 ctaaatctaa tttttcctga gtcatctctt cgccggtgat cgctttgaag aatttggctt
    35341 aaagcgccag atcgccccgg taattacggc ttttcagcgg cgaaacggtc attggccaga
    35401 cccagttgca cagagtgacg gcgttatgca aacagacccg caacagcgac cactttgcat
    35461 atttaatttt tgcgtcattg attggcgtgt agtttttggt ttcatcgtaa gcatcttcag
    35521 aaccaaaaag ttctttcgcc acttcacgtt gcagtttcaa tggcaagccg gaaccaataa
    35581 agttgatatg ggtatgcgtc atgcagtcac ggttgaacat acagttaaca atggaaccga
    35641 cctgcgctga cgcttcattg gcatggtgaa ccggatagcc aaacggcgac cagagtttat
    35701 ttttcgcgta gccccagtac tctttaccca aattccagcg ttctgcgatg gcatatgaac
    35761 catcagccag gtgactcagc tcacccacac gatgcgccag acggtagtaa aaatctttaa
    35821 tgaagttaac gtcacccgct tccagttgat cccagcgaat ttctgcatac tcttcagctg
    35881 gcagaacacg cttgaacaca cctttgctgt aacaataagt aaagtcgcga tgcaactgcc
    35941 cgtagttaca ccataggccg tagtcatcga acaaattcag accaaccagg ttaccaatca
    36001 cacgaccatc gtctttatct tcaaaatctt tcggcccgtt cgggaagatg gtggtatgga
    36061 caaagtttgc tacacaagtg ttaccacctg tgctgggcac gccaaactct ttcacccgag
    36121 gaatattcat ttgggtcata caacggatcg ggcaagagtg gcagccgctc atttttactg
    36181 tgtatttctc tgccgccggt cctaagtcaa aaacggattt ataggtacga aagccgaccg
    36241 tattctgatt gcctggcgga atttcacccg tttcaatcgg accaccttca gccgcgcccc
    36301 aaaacagccc tttacgtgct gtccagcgtg acttgggatc tgaatactct gcccacgatt
    36361 gtggcgtact tggcacgaca tggttgttat tcgcaccaat aagttcagtc atcatgtaat
    36421 cattgagacg cttcatctcc tgacgatcgg caatgttgac ccctttcgtc ccttcaaccg
    36481 caatcgcttt caggtttttc gaacccatta ttgcgccagt tcccgcaccg ccgctgtggt
    36541 tacggctatt caacatgcca gagagaggaa taaggttttc cccagcctga ccaatagccg
    36601 ccacacaggt ttccgggctg gtcaatcgac aaatttcttc cgtcgttgcg cgcgtccctt
    36661 ttccccataa gaaatcggct ttttccaggc taactttgtc atctttaatc ttcagccata
    36721 ccggtgattt cgccttccct tcgataataa tgacgtcgta tccagcgaat ttcatttgcg
    36781 ctgcaaaaaa gccacccata tgggcatcgc gactaaattt cctttggtaa aagtagaaag
    36841 tgaggtgata tttacgcgag aactacaggg ggcaccagat ccagttaatg ggccggtagc
    36901 aaagactaat ttattcgctt catcgaaagg tttcgtgcct agcggtactt cgtcatacat
    36961 aattttgtag ccgaagccca tgccaccgac aaaactttta aacttactgg aatcttcgag
    37021 ggtaatattt cctgtcgtga gattgactct taatatatta cctgtccaac cgttagccat
    37081 gattttttcc tttgcaagat tcacacagta atatctttcc actcgataat ttttaacgcc
    37141 cctgttgggc aggcgtttgc gcattcaccg cataacacac atttcgagga ttttttactt
    37201 tcggtattta cggtggccat catccacggg catgccgtag tacaggcgct acagccaata
    37261 caacgtttat gatcgacggt aatacagcct tctttctgct gccaggtaat cgcaccaatc
    37321 gggcagacgt tcatgcattg cggctctttg cattgacggc aggtgtccgc ggtatagttg
    37381 agatcgccat acaggccgcc gccagagcca accccgttgt cgccaaagaa ataattgcga
    37441 tggattttga tacgggagaa gaatgtccct actgagccat cgttgaagtt ggtacaagag
    37501 atttcacagc ggtggcaacc cgtgcatcgc gctcgctggg tcaccagcac ccctttgggt
    37561 gtatttatca accctactgt gccgctatca atatcttcct gtttgcaacc caaaagtgac
    37621 agtaacgcag gggcaattgt tagaccagca aggccttttc ctgatatacg caagaattct
    37681 aatcgcgtta agccaatatc taatagtgga cgatcaaccg ggttcattta tttcgcatcc
    37741 tcttcgcaaa cacagtgctg acaattcagc agcctgatat taagcattca actaatacgc
    37801 tgctgactct gtccccaaca ccagatattt tccctaaccc gatagtgtat gagaaaagat
    37861 ttaacgccga taaactaatc ctttatggtt aggagaaaaa ataatgtgat tattctgagc
    37921 ccttaacatt gatcgttatc aattaaaatt acaaagcaag aaattataag cgaactgata
    37981 tttattatca tttgaaataa atttaactta agcgacaatc atttgtgacg ttcattgcag
    38041 aaaataaaaa taacattatc gctataaatt aaaatacata ccgaaattcg tataatttca
    38101 gcagttgtta tttagaatga ttaactggac cgcaactgaa gcaagaatat caggatgaag
    38161 cttaccgcct catcctgaat ggtcatttta ccagtgcgtt aatgacggtt tccatcgcct
    38221 gcttttccag ctcactgcgc tgaatgcgtt tattggctaa tgctgtacgt aatacgcctg
    38281 cgataacaat atgctttggt tcctgcccca gatcgcgcat ttccagcaca actttaccga
    38341 caactctgca catttctcgg tacagttcat cctcttttgt ccgatttccc attctttata
    38401 cctatttatc atgccaacta tcaggtaatg ctgccaactt actgatttag tgtatgatgg
    38461 tgattttaag gtgcttgcgt ggcttccatt tccatcagat gtccttcctg ctccgctact
    38521 gaaggcgtgg t
//
LOCUS       DAEEUE010000029        37790 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.029, whole genome shotgun sequence.
ACCESSION   DAEEUE010000029 DAEEUE010000000
VERSION     DAEEUE010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37790
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.029"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..177)
                     /locus_tag="KLK84_001619"
     CDS             complement(<1..177)
                     /locus_tag="KLK84_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HBE2008985.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(261..1145)
                     /gene="yhdJ"
                     /locus_tag="KLK84_001620"
     CDS             complement(261..1145)
                     /gene="yhdJ"
                     /locus_tag="KLK84_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBE2008986.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1231..1527)
                     /gene="fis"
                     /locus_tag="KLK84_001621"
     CDS             complement(1231..1527)
                     /gene="fis"
                     /locus_tag="KLK84_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HBE2008987.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1553..2518)
                     /gene="dusB"
                     /locus_tag="KLK84_001622"
     CDS             complement(1553..2518)
                     /gene="dusB"
                     /locus_tag="KLK84_001622"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBE2008988.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2847..3728)
                     /gene="prmA"
                     /locus_tag="KLK84_001623"
     CDS             complement(2847..3728)
                     /gene="prmA"
                     /locus_tag="KLK84_001623"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBE2008989.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3740..5191)
                     /gene="panF"
                     /locus_tag="KLK84_001624"
     CDS             complement(3740..5191)
                     /gene="panF"
                     /locus_tag="KLK84_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HBE2008990.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5181..5423)
                     /locus_tag="KLK84_001625"
     CDS             complement(5181..5423)
                     /locus_tag="KLK84_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HBE2008991.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5532..6881)
                     /gene="accC"
                     /locus_tag="KLK84_001626"
     CDS             complement(5532..6881)
                     /gene="accC"
                     /locus_tag="KLK84_001626"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBE2008992.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGKRCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6892..7362)
                     /gene="accB"
                     /locus_tag="KLK84_001627"
                     /gene_synonym="fabE"
     CDS             complement(6892..7362)
                     /gene="accB"
                     /locus_tag="KLK84_001627"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBE2008993.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8340..9314)
                     /locus_tag="KLK84_001628"
     CDS             complement(8340..9314)
                     /locus_tag="KLK84_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBE2008994.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9466..11406
                     /gene="csrD"
                     /locus_tag="KLK84_001629"
     CDS             9466..11406
                     /gene="csrD"
                     /locus_tag="KLK84_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HBE2008995.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11711..12754
                     /gene="mreB"
                     /locus_tag="KLK84_001630"
     CDS             11711..12754
                     /gene="mreB"
                     /locus_tag="KLK84_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HBE2008996.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12820..13923
                     /gene="mreC"
                     /locus_tag="KLK84_001631"
     CDS             12820..13923
                     /gene="mreC"
                     /locus_tag="KLK84_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBE2008997.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13923..14411
                     /gene="mreD"
                     /locus_tag="KLK84_001632"
     CDS             13923..14411
                     /gene="mreD"
                     /locus_tag="KLK84_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBE2008998.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14420..15013
                     /gene="yhdE"
                     /locus_tag="KLK84_001633"
     CDS             14420..15013
                     /gene="yhdE"
                     /locus_tag="KLK84_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HBE2008999.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15003..16472
                     /gene="rng"
                     /locus_tag="KLK84_001634"
     CDS             15003..16472
                     /gene="rng"
                     /locus_tag="KLK84_001634"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HBE2009000.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16540..20340
                     /gene="yhdP"
                     /locus_tag="KLK84_001635"
     CDS             16540..20340
                     /gene="yhdP"
                     /locus_tag="KLK84_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HBE2009001.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAAGKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20496..21941
                     /gene="tldD"
                     /locus_tag="KLK84_001636"
     CDS             20496..21941
                     /gene="tldD"
                     /locus_tag="KLK84_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HBE2009002.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22069..22998)
                     /gene="aaeR"
                     /locus_tag="KLK84_001637"
     CDS             complement(22069..22998)
                     /gene="aaeR"
                     /locus_tag="KLK84_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HBE2009003.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23181..23384
                     /locus_tag="KLK84_001638"
     CDS             23181..23384
                     /locus_tag="KLK84_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HBE2009004.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23392..24324
                     /gene="aaeA"
                     /locus_tag="KLK84_001639"
     CDS             23392..24324
                     /gene="aaeA"
                     /locus_tag="KLK84_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HBE2009005.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24330..26297
                     /gene="aaeB"
                     /locus_tag="KLK84_001640"
     CDS             24330..26297
                     /gene="aaeB"
                     /locus_tag="KLK84_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HBE2009006.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26389..26661
                     /locus_tag="KLK84_001641"
     CDS             26389..26661
                     /locus_tag="KLK84_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HBE2009007.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26717..26980)
                     /gene="yhcN"
                     /locus_tag="KLK84_001642"
     CDS             complement(26717..26980)
                     /gene="yhcN"
                     /locus_tag="KLK84_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HBE2009008.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27345..27815)
                     /gene="argR"
                     /locus_tag="KLK84_001643"
     CDS             complement(27345..27815)
                     /gene="argR"
                     /locus_tag="KLK84_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HBE2009009.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28250..29188
                     /gene="mdh"
                     /locus_tag="KLK84_001644"
     CDS             28250..29188
                     /gene="mdh"
                     /locus_tag="KLK84_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBE2009010.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29250..30317)
                     /gene="degS"
                     /locus_tag="KLK84_001645"
     CDS             complement(29250..30317)
                     /gene="degS"
                     /locus_tag="KLK84_001645"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HBE2009011.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30407..31754)
                     /gene="degQ"
                     /locus_tag="KLK84_001646"
                     /pseudo
     CDS             complement(30407..31754)
                     /gene="degQ"
                     /locus_tag="KLK84_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31908..32306)
                     /locus_tag="KLK84_001647"
     CDS             complement(31908..32306)
                     /locus_tag="KLK84_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HBE2009012.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32500..33627
                     /gene="zapE"
                     /locus_tag="KLK84_001648"
     CDS             32500..33627
                     /gene="zapE"
                     /locus_tag="KLK84_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HBE2009013.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33847..34275
                     /gene="rplM"
                     /locus_tag="KLK84_001649"
     CDS             33847..34275
                     /gene="rplM"
                     /locus_tag="KLK84_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBE2009014.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34291..34683
                     /gene="rpsI"
                     /locus_tag="KLK84_001650"
     CDS             34291..34683
                     /gene="rpsI"
                     /locus_tag="KLK84_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBE2009015.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35078..35716
                     /gene="sspA"
                     /locus_tag="KLK84_001651"
     CDS             35078..35716
                     /gene="sspA"
                     /locus_tag="KLK84_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HBE2009016.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35722..36219
                     /gene="sspB"
                     /locus_tag="KLK84_001652"
     CDS             35722..36219
                     /gene="sspB"
                     /locus_tag="KLK84_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HBE2009017.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVCFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36262..37629)
                     /gene="dcuC"
                     /locus_tag="KLK84_001653"
     CDS             complement(36262..37629)
                     /gene="dcuC"
                     /locus_tag="KLK84_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBE2009018.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(37650..>37790)
                     /locus_tag="KLK84_001654"
     CDS             complement(37650..>37790)
                     /locus_tag="KLK84_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE2009019.1"
                     /translation="TQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKH
                     YQ"
BASE COUNT         9152 a         9455 c         9932 g         9251 t
ORIGIN      
        1 cattctgtcc tgaaaattca gttcgtcaca gttaaccagt tttagtggat taactgagtt
       61 catattacgg catttgtctg tttctgttgt cagcagttca atccactgca tcagtaaagt
      121 atcaaacaga aaaacgaaga cagcgaaaac gacgagccac caatacttgc gaatcattac
      181 ctgagcctgt gcatgtttaa ccaagtctgg caggaaatat acacgaaatg aataatctga
      241 aaactaggct tacacttcct ttactttgca atgagatcgg ggtcaacttc gcttaaccgg
      301 cttcgttttg acaggttgcc ggtctttctt tttttcactt tcgccagttc ttccgcggag
      361 taatgcgacg cgacatccag ccgtcgaagc cccattttga tgtactcgct gttgatctca
      421 ataccaatga attttcgtcc gctggcgatg gctacggcac cggtagtaaa gctaccggca
      481 aacgggtcga gaacgatatc gcctgggttg gaagaggcga gaataatgcg tttcagtaag
      541 gctttcggtt tttgcgtcgg gtggttctca tattcatcca tcaaataacg cacgcgcgga
      601 aaatcccaga cgttacccgg tactttttga tgattgtatg gctgtggagg atttttgcga
      661 taatcgatca acgcgcgctg cgatccggtt ttggcttcta ccagaatagc atcaccgttg
      721 aatgtgtagt tctttgcgtc tttcaccatc atcaggatgg gttcgtacat ggagccgtag
      781 tgttttttcg cctgtactcc agaactgtca tatgaccaga cgatgcgact tttgatggta
      841 aaaagcttgc ggcactggag atcgataaag ggcatgtttt ccgtactgtt cataatgtac
      901 atgctgccct gctttttcag aacgcggtgg cactctgcaa tcacttcaaa cagccagtcg
      961 ataaacagat cttctttcca ggcttcgatc agaccatcaa aatttttacc gatgttatac
     1021 ggtgggtcgg caaagagcag atcgacgctt tcagtaggta gctttttaag ttcggcaaag
     1081 gcatcaccgt gaataatggt cttagcttca ttaccaaacc gggtcggttc acatcctgtt
     1141 ctcatggtca ctccctttgt gacacctata aaaaaggcgc ttccccatac cgagtagcgc
     1201 ctttttaatc aagcatttag ctaacctgaa ttagttcatg ccgtattttt tcaatttttt
     1261 acgcagcgta ccacggttga tgcccatcat cagcgcagca cgggtctggt taccacgggt
     1321 gtattgcatc accatgtcca acaggggctg ttctacttca gccagtacca gctcatagag
     1381 gtcattcaca tcctgaccat tcagttgagc aaaatagttc ttcagtgcct gtttaaccga
     1441 gtcacgcagg ggtttttggg ttacctgatc ctgagagtta acggtagaaa cggtcagtac
     1501 gtcagaattt acgcgttgtt cgaacatagt tctgtcagct ctttatttct gtttacgcaa
     1561 aattttcgaa gtatgcctcc aacgcctcca gctgttcgct ggcatcctca atggcgttga
     1621 atgtgcgccg aaactggtca tttggagcgt gttcctggag ataccaggaa acgtgtttac
     1681 gtgcaattcg gtaccctttt gccggaccat aaaagtcatg cagttcccga acgtgcgcgc
     1741 aaagcaagcg cttaacctct gccaaaggca gtgggggcag caactcccca gtgtccagat
     1801 aatgctggat ttcccgaaag atccagggtc ttccctgagc tgcgcggcct atcatcaggg
     1861 catccgcccc tgtatagtcg agcacagctc tggctttaag cgggtcagta atgtcgccat
     1921 tcgcgataac cggaatggaa actttctgct taactgcccg aatactgtcg tactcagctt
     1981 ctccattgaa caaacaggcg cgtgtacggc catgaatggt cagagcctga atgccacagt
     2041 cttcagccag ttgggcaatc tcttcgcagt tacggtgttc cggtgcccag ccggtgcgaa
     2101 tcttcagggt aacaggaacg tccactgcat tgacgacctc ggtaaggatc gatttaacga
     2161 catccgggta ctgcaagagg gctgaacctg cgagcttgcg attcactttt ttagccgggc
     2221 aacccatatt gatatcaata atctgggcac cgctttccac gttaatacgt gctgcatctg
     2281 ccatttcttt cggagcgcta ccagcaattt gcacggtgcg aataccgggt tcatcaatgt
     2341 gcaccatccg taaacgagat ttgtcgcttt cccaaacctg tgggttagaa gacatcatct
     2401 cggatactgt caatccggct cccatctcgt agcacaacgt ccgaaaaggt ctgtctgtaa
     2461 tgccagccat gggcgctgcg atcaggcgat ttctgagctg atattgtccg atgcgcatga
     2521 gttaagaaat gaccatactg tgactgcaag gcggcgtata ttacgcattt tttgcacgag
     2581 atgaaaggcc aaactttgac caatcctctg agatggatca aagaattgca tttaaaatga
     2641 gcgtggtgcg ataattactc ataaaaatca tcatattaga aaatagtgac taaaatttac
     2701 actcaaagaa atttgagtaa gttctcaatt tttctttatg aatgaaaatt ttggcacgca
     2761 aatttgcgta aataatcggc aaatttacgt gccttttgtg agcttgctcg cacttcgccc
     2821 cgcgtcaccc tacggcgatg cgaaggtcaa ttcttacgac cggtaatacg gcaccactct
     2881 tctttttcca cgaccgggtc cagtgcgaag ctatcggcat aagcttcaca aacgctctct
     2941 gcctggcttg ccagaatacc ggaaaggccc agcaaaccgc ctgaaaccgg caggacgctg
     3001 attaacggtg ccagttcacg taatgggcct gcaaggatgt tagcgaccac cacgtcggct
     3061 ttcatttctt ctggctgatc tttcggtaag tagagttcca gacggtcaga aacgccatta
     3121 cgttcggcgt tatcgcggct ggcctgaatc gcctgcggat cgatatcaat accaatggct
     3181 tttgctgcac ccagtttcag cgccgcgatc gccagaatgc cggaaccaca gccaaagtcg
     3241 atgactgttt taccggttaa atcgaggctg tcgagccatt gcaggcacag agaggtggtt
     3301 ggatgggtac ccgtaccaaa cgccagccct ggatctaaca tcacgttgac ggcgttttcg
     3361 tccggcacat cacgccagct agggcagatc cacagtcgtt caccaaagcg catcgggtgg
     3421 aaattatcca tccattcgcg ctcccagtct ttatcttcta gttgttcgat tttatgcgcg
     3481 aagcctgcgc cgagcagcgg atggttttcc agaatcgcca ccacgtcgtt catatcggtt
     3541 tcagcgtcga acagaccaat cacatcggtg tcgccccaca ggcgcgtttc gcccggcagc
     3601 ggctcaaata ctggcgtatc gtgggtatcc tgaaaagtga tagaaacggc acccgcttcc
     3661 atcagcgcat cgctaagatc ttccgcgttc gcgccggtgg tgttcagttt cagttggatc
     3721 caaggcatgg caaaactctt tatttatcag tagtcaaaac ggtagcttgc gggacggatg
     3781 taccgaaacg gtttccgacc aggaaagcca gcaaacttag tagtaacgag ggcacgatag
     3841 ggtggaagcc caggtactga atattcagcg tcgcgagtac ggcatacagc acgccgccaa
     3901 cgatcatcgc acttagcgcg cctttggcgt tggcgcgttc ccagtaaaga cccagcacca
     3961 gcggccacag gaaaacggct tccagcccac cgaaggccag caaattcagc cagatgatca
     4021 tttctggcgg cttccaggcg gcaagcagca gcaacgcgcc gagaactaac gtaattaccg
     4081 ccgacatccg cttcagacgc gtctcgtttt gcatttgatc cggacggata ttcagataga
     4141 gatctttaat gatcgtagcg gaactttgca gcagttgggc gttaattgtc gacatgatcg
     4201 cagccatagg tgcagccaga aagatcccgg cagcaaaagg tggcagcact tttaccatta
     4261 acgtcgggat caccagatcc ggtacggtga gatcggggat cactgcacga cctaacgctc
     4321 cggccaggtg cataccgaac atcaggattg cgactataat cgtaccgatg atgatccccc
     4381 ggtgtactgc tttgctgtct ttataagaga tacagcgcac cgcagtatgc ggcaggccaa
     4441 tcacgccaaa gcacaccagt acccagaacg acgtcataaa ggcaggcgac agaatatcgt
     4501 cagcgccttg tggcgtaacc agttgcggat cgatggtttg caaggtctgt actgcgttgc
     4561 ttaagccgcc agcggcatgt accacgccaa taagcagcac aacggtgcca atcagcatca
     4621 caagcccttg catggtgtcg ttcagcacgc tggcgcgaaa gccaccaaag gcggtatata
     4681 acgcaatgct gataccaaaa atcagcagcc cggtttcata aggaataccc gccgcggttt
     4741 ccagcaggcg cgcaccgccg ataaactgca cggtcatcgc accaacgaac gcaaccagca
     4801 aactcaaact cgccagccac accagaagac gactctggta gcgggcaaac agcatatcgt
     4861 tcagcgtcac tgcattgtag cggcgcgcaa gaatcgcaaa cttcttgccg agaataccga
     4921 gtgaaagcca gacagcagga agctgaatca tcgccagcaa tacccagccc agcccgtatt
     4981 tataagcagc tcctggcccg ccgataaacg aactggcact gatataggtc gcggtgagcg
     5041 tcatcgccag cacaataccg cccatagagc ggctgccgag gaaatactca ttaaggaagg
     5101 tgccggtgct ccgtttacgc atcgcataaa ccgagatacc gaacaccacc accagatagg
     5161 cgaccagcgg tagaattact tcaagctgca tcgtcatcct ccagtgggat atcgcgatag
     5221 ataaatttca ccatcgccca gcacagtcca ataaacagta gcggcgtcag gatgcaggcc
     5281 atctcaaacc agcgcggaaa gccggtaaaa ccgggggcaa cgccaggtaa gtaagcgtct
     5341 actaaccaaa ctgccagata caaaagggtc agccccagcg cccagcgcgc ctctttatgg
     5401 gcctgaacaa aacgagtgtc cattttttgt ccctgatggg tgaagaaagc gaggattgta
     5461 ccttatgggg gttgtcgatc cccagtaata aaaaaggccg gaaaatccgg ccttttgacg
     5521 cttttgcagt cttatttttc ctgaagaccg agttttttct ccagatagtg gatgttagtg
     5581 ccaccatgct ggaagttctc gtcattcatg atgcggatct gcagatcaac gttggttttg
     5641 ataccgtcga tgatcagctc ctgcagcgca ttcttcatgc gggcaatcgc cacgtcacgg
     5701 ttttcaccgt agcaaatcag cttaccgatc attgagtcat agtacggcgg tacggtgtag
     5761 cccgcgtaga tatgagactc ccaacgtacg ccaaaaccgc caggtgcgtg gaaacgggtg
     5821 attttgcctg ggcttggcag gaaggtgttc ggatcttcgg cgttgatacg acattccacc
     5881 gcatggccgc gaacgtgaac ttcttcttgc ttgatcgaca gcggttgacc ggcagcgata
     5941 cgcagctgtt ctttgatcag gtcaacgccg gtgatcattt ctgtaaccgg gtgttctacc
     6001 tgaatacggg tgttcatttc gatgaaatag aactcgccgt tttcgaacag gaactcgaaa
     6061 gtacctgcac cgcgatagcc gatatcaaca cacgctttag cgcaacgttt gccgatgtag
     6121 cgacgcagtt ccggggtaat gcccggtgct ggtgcttctt cgaccacttt ctggtggcgg
     6181 cgctgcatgg agcagtcacg ttccgccaga tagatagcgt tgccctgacc gtcagccagt
     6241 acctgaatct cgacgtggcg aggattctcc aggtatttct ccatgtaaac catatcgttg
     6301 ctgaaagcag ctttcgcttc cgcacgggtc atggagatgg attgtgccag ttcagcgtcg
     6361 ccgcgcacta cgcgcatacc gcgaccgccg ccgccgccgg aggctttgat aatcaccgga
     6421 taaccaatgc gtttagcaat ggcgcggttt ttatccatat cgtcgcccag cgggccgtca
     6481 gaaccaggta cgcaaggaac gcccgctttt ttcatcgccg cgattgcgga tactttgtcg
     6541 cccatcaggc gaatggtttc tgctttcggg ccgatgaaga taaagccgga gcgttcaacc
     6601 tgctcggcaa agttggcgtt ctcggagagg aagccgtaac ccggatggat tgctactgcg
     6661 ccggtgattt cagcggcgct gatgattgcc gggatgttca gataactttt tactgacgga
     6721 gcagggccaa tacagaccgt ttcatctgcc agtaatacgt gttttagatc gcgatccgtg
     6781 ctggagtgca cagcgacagt cttgatgccc agttctttac aggcacgaag aatacgcaat
     6841 gcaatctcgc cgcggttggc aataacaatt ttatccagca tgttcgcctc gttactcgat
     6901 gacgaccagc ggctcgtcaa attctaccgg ttgtccactt tcgaccagaa ttgctttcac
     6961 ggtaccggat ttgtccgctt cgatctggtt catcattttc atggcttcaa cgatgcacag
     7021 ggtatcgccc acgttgactt tctgacccac ttcgatgaac gcttttgcgt ccgggcttgg
     7081 ggtgcggtag aaagtaccaa ccatcgggga acgtacgatg tgaccactga tttccgctgc
     7141 tgctggcgct tccatggaag gaacggtcgc cggagcggct gcgttagatt gagctggctg
     7201 ctgcatcatt ggtgcagcgt aagcttgttg catcacaggg aaacttgcgg caggagctgc
     7261 acggctaatg cgtactgact cttcgccttc agaaatttcc agttcggaga tgcctgattc
     7321 ttcaaccagc tcgatcagtt ttttaatctt acgaatatcc atgagtgggt tccgtactct
     7381 ttgtttagtg tgattgtgac aggcgtttca ccgccgtctg taaagcgtat gccccgacag
     7441 ctgtatgcat agcgataaat tccagcaggc cgggacgccg gtctattttg ccccagagtg
     7501 tcaatgttag acttgacgga cattgtgcag cgatctgctg ctatcctccg gcaaaaaaca
     7561 aaatatacct tcgttgcgcc aatgtcacct tttccgcagc gcaaaaactg cgtcagggag
     7621 gacgaggtcg cacattataa cgatttcgta gcaattggca gctaaatact ggtcttatca
     7681 gggaatataa tcaacagcta acatgtaaat aaccttcaac accgtgtaat ttgcaacaag
     7741 ccgcacaatt cacgaaatta gcgccatcat cgacggaact tcttataacg taaggtgaaa
     7801 agctgaaaag ccagccctgc gaagatgagg ggctgcaatg agataatctt cacagaccac
     7861 aaataatgta gggatgccag gatcgcgaca agatagacga cattacgcag cggttgctgg
     7921 ggattaccca attttcactg catcgtctgg atagatgtga atgctaaagc acgcaaaatc
     7981 aaccaactga caattctcag cattaaatag gttcgagtgg ttaatttgta gtcaaataaa
     8041 atcagattat tcaccccatg gtaccagaaa tcagtaacag gttaagcgta gtgtcgccca
     8101 ggcaaagcac cataaaccta acaggcgacg gaggcgtatc aataatggct gtttagagga
     8161 gtatcaatgc gcatcaattt gtggtcagtc gaaatcatga ctttccagtt tggatgatgt
     8221 tgtacaactt cctgaccaag gtgttcgacg aaagctgtcc ggctaaccgc ttttcccgtt
     8281 aaccgattga ataatgatgt caaaatgagt tccctctctt attattcctt gctaaatggt
     8341 tagttaacct tcaccagcgt gcgaccctgg atctggttat taatgatggc ctcggcaaag
     8401 ttcggtgcct ctgacagaga tatctctttt gccgcctggg tatagaatga ttccggtaaa
     8461 tcggcgacca gtcgctgcca ggcttgtgcg cggcgttctg gtggcgtcat tactgaatcc
     8521 accccttgca aacggacatt acgcagaata aatggcatga ccgtggttgg cagagtaaaa
     8581 ccacccgcca gaccacaggc cgccacgcag ccgccgtaat tcatttgcgc cagcactttt
     8641 gccagcactt tgtcgccaac agtgtcaatt gccccagccc agacttgttt ttccagagga
     8701 cgggattcgg caaactcatc acgagggaga ataccgctgg cacctaaact tttcagatat
     8761 tcatgggtac tttcgcgacc ggaaacggca acgacctgat aacccaactt atgcagcagc
     8821 gccacggcgg tactgccgac gccaccactg gcacccgtca cgacaatctc cccgtcctgc
     8881 gggcgaacac cggcatcttc cagcgccatc acacacagca tggcggtaaa accggcagta
     8941 ccgataatca ttgctttacg cgcgtccagc ccttgcggca tggcaaccag ccagtcacct
     9001 ttcactcgcg cctgctccgc cagcccaccc cagtggtttt caccaacgcc ccagccagtg
     9061 agtaacacct cctgaccggc atgaaaacgc ggatcttcgc tggtgcgtac agttccggca
     9121 aaatcgatcc caggaatcat cggaaaatta cggatgattt ttcccttacc ggtaatcgcc
     9181 agcgcatctt tatagttcag gctcgaccag tgaacatcga ccgtgacatc gccctccggc
     9241 aggcgacgtt cgtccagagt ctgtactgat gcgagagttt tgccgtcctg ctgttctaaa
     9301 agtaacgcct gcataagtgg tcctcatgtg catgatggat tggaaaataa tttctgaaga
     9361 ctatactcgc taatggaaaa gcaaagccga tgaagcgcaa taaattgcga gataaatctg
     9421 atttgctagt atgcccgctt cctcactatc ggagttaaca caaggatgag attaacgacg
     9481 aaattttcgg cctttgttac gctgctcacc gggttaacaa tttttgtgac tttgctgggc
     9541 tgttcgctaa gtttctacaa cgccattcag tataagttta gtcatcgcgt tcaggcggtg
     9601 gcgacggcga ttgataccca ccttgtgtcg aatgacttca gcgcattaag gccacaaatt
     9661 accgaattaa tggtgtcagc agatatcgtt cgtgtagacc tgctgcatgg tgataaacag
     9721 gtttataccc tggccagaaa tggtagttat cgtccggttg gcaccagcga tctgtttcgt
     9781 gaactgagcg ttccgttgat aaagcatccg gggatgtcgt tgcgtctggt ttatcaggat
     9841 ccgatgggca actatttcca ttcgttgatg accaccgcgc cgctcacggg ggcgattggc
     9901 tttatcattc ttatgctctt tctggcggta cgctggttac aacggcaact tgccgggcaa
     9961 gaattgctgg aaacccgggc tactcgtatc ttaaacggtg agcgtggctc taatgtgttg
    10021 ggaaccatct atgaatggcc gcccagaacc agcagtgcgc tggatacgct gcttcgtgaa
    10081 attcagaacg cacgcgaaca acacagccgt cttgatacgc tgatccgctc ttatgccgca
    10141 caggacgtga aaaccggcct caataaccga ctctttttcg ataatcagtt agcaacgtta
    10201 ctggaagatc aggagaaagt aggtacccac gggatcgtga tgatgattcg tctgccggat
    10261 ttcaatatgt tgagcgatac ctgggggcac agccaggttg aagaacagtt cttcactctg
    10321 acgaatctgc tgtcgacatt tatgatgcgc taccctggcg cactgctggc gcgttaccac
    10381 cgcagtgatt ttgctgcgct gttaccgcac cggacgttaa aagaggcaga gagcatagcc
    10441 agtcagttaa tcaaagccgt cgataccttg ccgaacaata aaatgctcga tcgcgacgat
    10501 atgatccaca ttggtatctg tgcctggcgt agtggtcagg ataccgagca ggtaatggaa
    10561 catgcagagt ctgccacgcg taatgcggga ttgcagggtg gcaatagctg ggctatttat
    10621 gatgactcgt tgcctgaaaa aggacgcggt aatgttcgct ggcgtacgct tatcgagcaa
    10681 atgctcagtc gcggcggccc gcgcctttat caaaaaccgg cggttactcg cgaaggtcag
    10741 gttcatcatc gcgaactcat gtgccgcatc ttcgatggta atgaagaggt tagctcggcg
    10801 gagtatatgc cgatggtctt gcagtttggc ttatcggaag agtatgaccg tctgcaaatc
    10861 agccgtctta ttccactatt gcgttactgg ccagaggaaa atctggcgat tcaggttacc
    10921 gttgagtcgc tgattcgtcc gcgttttcag cgttggctgc gcgatacgtt aatgcaatgt
    10981 gaaaaatcac aacgaaaacg cataattatt gaacttgcag aggccgatgt aggtcaacat
    11041 atcagtcgtt tacaacctgt tattcgttta gtgaatgctt taggggtacg ggtagccgtc
    11101 aaccaggctg gtttgacgct ggtaagcacc agttggatca aagaacttaa tgttgagtta
    11161 ctcaagctcc atccggggct ggtcaggaac attgagaagc gaacggagaa ccagctgctg
    11221 gttcaaagcc tggtggaagc ctgctccggg accagcaccc acgtttacgc caccggcgtg
    11281 cgttcgcgaa gcgagtggca gaccctgatt cagcgcggtg ttacaggcgg gcaaggggat
    11341 tttttcgcgt cctcacagcc acttgatact aacgtgaaaa aatattcaca aagatactcg
    11401 gtttaacctg ccgtttaatc cgttttcacg tagaataacg cgcgctgcgt ctcatgggag
    11461 tgtgcttgtc tgctcgccag attgttgcag cacatatgca gatgaatgac cttacgcggt
    11521 tgcaaacagg cgaggaatgc tgctgatgca ttaagccttt ctggactcag gcagagattt
    11581 gtaacaaagg aaacgaactg cactaatttt caccgtagca gatgattttt gcgccttgtc
    11641 gctgctgcgt gtggttggta aagtaagcgg attttctttt ccgccccagc tttcaggatt
    11701 atcccttagt atgttgaaaa aatttcgtgg catgttttcc aatgacttgt ccattgacct
    11761 gggtactgcg aataccctca tttatgtaaa aggacaaggc atcgtattga atgagccttc
    11821 cgtggtggcc attcgtcagg atcgtgccgg ttcaccgaaa agcgtagctg cagtaggtca
    11881 tgacgcgaag cagatgctgg gccgtacgcc gggcaatatt gctgccattc gcccaatgaa
    11941 agacggcgtt atcgccgact tcttcgtgac tgaaaaaatg ctccagcact tcatcaaaca
    12001 agtgcacagc aacagcttta tgcgtccaag cccgcgcgtt ctggtttgtg taccggttgg
    12061 cgcgacccag gttgaacgcc gcgcaattcg tgaatccgcg cagggcgctg gtgcccgtga
    12121 agtcttcctg attgaagaac cgatggctgc cgcaattggt gctggcctgc cggtttctga
    12181 agcgaccggt tctatggtgg ttgatatcgg tggtggtacc actgaagttg ctgttatctc
    12241 cttgaacggc gtggtttact cctcttccgt gcgcattggt ggtgatcgtt tcgacgaagc
    12301 tatcatcaac tatgtgcgtc gtaattacgg ttctctgatc ggtgaagcca ccgcagaacg
    12361 tatcaagcac gaaatcggtt cggcttaccc gggcgatgaa gtccgtgaaa tcgaagttcg
    12421 tggccgtaac ctggcagaag gtgttccacg cggttttacc ctgaactcca acgaaatcct
    12481 cgaagcactg caggaaccgc tgaccggtat tgtgagcgcg gtaatggttg cactagaaca
    12541 gtgcccgccg gaactggctt ccgacatctc cgagcgcggc atggtgctca ccggtggtgg
    12601 cgcactgctg cgtaaccttg accgtttgtt aatggaagaa accggcattc cagtcgttgt
    12661 tgctgaagac ccgctgacct gtgtggcgcg cggtggcggc aaagcgctgg aaatgatcga
    12721 catgcacggc ggcgacctgt tcagcgaaga gtaatcggat gcaggcaggg gaagcgtctg
    12781 tttaccctgc ctggtctgat acgagaatac gcataactta tgaagccaat ttttagccgt
    12841 ggcccgtcgc tacagattcg ccttattctg gcggtgctgg tggcgctcgg cattattatt
    12901 gccgacagcc gcctggggac gttcagtcaa atccgtactt atatggatac cgccgtcagt
    12961 cctttctact ttgtttccaa tgctcctcgt gaattgctgg atggcatatc gcagacgctg
    13021 gcctcgcgtg accaactaga acttgaaaac cgggcgttac gtcaggaact gttgctgaaa
    13081 aacagtgaac tgctgatgct tggacaatac aaacaggaga acgcgcgtct gcgcgagctg
    13141 ctgggttccc cgctgcgtca ggatgagcag aaaatggtga ctcaggttat ctccacggtt
    13201 aacgatcctt atagcgatca agttgttatc gataaaggta gcgttaatgg cgtttatgaa
    13261 ggccagccgg tcatcagcga caaaggtgtt gttggtcagg tggtggccgt cgctaaactg
    13321 accagtcgcg tgctgctgat ttgtgatgcg acccacgcgc tgccaatcca ggtgctgcgc
    13381 aacgatatcc gcgtaattgc agccggtaac ggttgtacgg atgatttgca gcttgagcat
    13441 ctgccagcga atacggatat tcgtgttggt gatgtgctgg tgacctccgg tctgggcggt
    13501 cgtttcccgg aaggctatcc ggtcgcggtt gtctcttccg taaaactcga tacccagcgc
    13561 gcttatactg tgatccaggc gcgtccgact gcaggactgc aacgtttgcg ttatctgctg
    13621 ctgctgtggg gcgcggatcg taacggcgct aacccgatga cgccggaaga ggtgcatcgt
    13681 gttgctaatg aacgtctgat gcagatgatg ccgcaggtat tgccttcgcc agacgcgatg
    13741 gggccaaagt tacctgaacc ggcaacgggg atcgctcagc cgactccgca gcaaccggcg
    13801 acaggaaatg cagctactgc gcctgttgcg ccgacacagc ccgctgctaa tcgctctcca
    13861 caaagggcta cgccgccgca aagtggtgct caaccgcctg cgcgtgcgcc gggagggcaa
    13921 tagtggcgag ctatcgtagc cagggacgct gggtaatctg gctctctttc ctcattgcgc
    13981 tgttgctgca aatcatgccc tggccggata acctgattgt tttccggcca aactgggtgt
    14041 tacttatctt gttgtattgg atcctggcct tgccccatcg cgtaaatgtg ggcacaggtt
    14101 ttgtgatggg tgccatactg gatctgatca gcggctcgat gcttggcgta cgcgtattgg
    14161 cgatgagcat cattgcttac ctggtggcgc tgaaatacca gcttttccgc aacctcgcat
    14221 tatggcagca ggcgctggtc gtcatgttgc tttcgctggt ggtggatatt attgttttct
    14281 gggcagagtt tttagtgatt aacgtctctt tcagaccgga agtgttctgg agtagtgtag
    14341 tcaatggggt gctctggccg tggattttct tgctgatgcg taaagtccgt cagcagtttg
    14401 cagtgcaata aaggtttcta tgacttctct gtatttagct tccggttctc cgcgtcgtca
    14461 ggagttactt gcgcaacttg gcgtgacctt tgaacgtatt gttacgggca ttgaggagca
    14521 gcgtcagccg caggagagcg cgcagcagta tgttgtgcgt ctggcacgcg agaaagcaca
    14581 ggcgggtgtc gcgcaaacgg cacaggatct cccggtgctg ggtgcggata ttatcgttat
    14641 cctgaacgga gaagtgctcg agaaaccgcg cgacgcagag catgcggcgc agatgttgcg
    14701 caaattatcg ggtcagaccc atcaggtgat gacggcagtg gcgttggccg acagccagca
    14761 cattctcgat tgtctggtgg tcaccgatgt gactttcaga acgttaacag acgaagacat
    14821 cgcgggctat gtcgccagcg gtgaaccgtt agataaagca ggtgcatacg gtattcaggg
    14881 gctgggtggc tgttttgtca ggaagataaa tggcagctat cacgccgtag tcggcttacc
    14941 gctggttgaa acgtatgaat tattaagtaa ttttaacgca ctgcgtgaga aaagggataa
    15001 acatgacggc tgaattgtta gtaaacgtaa cgccttcgga aacgcgagtg gcgtatattg
    15061 atggcggtat tctgcaggaa attcatattg aacgtgaggc gcgacgcgga atagtaggca
    15121 atatctacaa gggtcgtgta agtcgtgtac ttccgggtat gcaggcggct tttgtagata
    15181 ttgggctgga taaagccgcg tttcttcatg catccgacat catgccgcat accgaatgtg
    15241 tggcgggtga agaacaaaag caattcacgg tgcgcgacat ctcggaactg gttcgtcagg
    15301 ggcaagatct gatggtgcag gtggtgaaag atccgcttgg cactaaaggt gcgcgcctga
    15361 ccaccgatat cacgctgcct tctcgctatc tggtatttat gccaggggct tctcacgttg
    15421 gggtttccca acgaattgaa agcgaatcag aacgtgaacg tctgaaaaaa gtggtcgcag
    15481 agtattgcga cgagcagggc gggtttatca tccgcaccgc agcggaaggg gttggcgagg
    15541 ctgaactggc ctccgatgcc gcttatctga aacgcgtctg gaccaaagtt atggagcgta
    15601 aaaagcgccc gcagacccgt tatcagctgt acggcgaact ggcgctggcg cagcgtgttc
    15661 tgcgtgattt tgccgacgcc gaactggacc gcattcgcgt tgactcacgc ctgacttacg
    15721 aagcattgct ggagtttacc tcggagtaca ttcccgagat gaccagcaag ctggagcatt
    15781 acaccggacg ccagccgatt ttcgatctct ttgatgtcga aaacgaaatc cagcgagcgt
    15841 tggaacgcaa agtagaactg aaatccggtg gttatctgat tatcgaccag accgaagcga
    15901 tgaccaccgt ggacatcaat accggagcgt ttgtcggtca tcgcaatctg gacgacacta
    15961 ttttcaatac caatattgaa gcgacgcagg ctatcgctcg ccagttacgg ttgcgtaatc
    16021 tgggcgggat tatcattatt gatttcatcg atatgaataa tgaagatcac cgccgccgcg
    16081 tgctgcattc gctggagcag gcgttgagca aagaccgggt gaaaaccagc gttaatggtt
    16141 tttcggcgct ggggctggtg gagatgacgc gtaaacgcac ccgcgaaagc attgagcacg
    16201 tactgtgtaa cgaatgccca acctgccacg gtcgcggaac ggtgaaaacc gtggaaacgg
    16261 tatgctatga aatcatgcgc gagattgttc gtgtccacca tgcttacgac tccgaccgtt
    16321 tcctggtcta tgcttctccg gcagtagccg aagccttgaa aggcgaagag tcacactcgc
    16381 tggcggaagt ggaaattttc gttggcaaac aggttaaagt acaaattgaa ccgctctata
    16441 accaggagca gtttgacgtc gtaatgatgt aaacagatgc tgggccgcca tccggcaaag
    16501 ggtttttgag tcacattttt agcagacaag gagtgacggg tgaggcgatt gccggggatt
    16561 ttactgctta ctggagccgc gctcgttgtg atcgctgccc tgctggttag cggcctgcgt
    16621 attgctttac cgcatcttaa cgcctggcgt ccggaaatcc tcaacaaaat agaatccgcg
    16681 actggcatgc cggtagaagc cagtcagctc tcagccagct ggcagaattt tggcccgacg
    16741 cttgaagcac acgacatccg tgcagaacta aaagatggcg gcgaattttc ggttaaacgc
    16801 gttactctgg cgctggatgt ctggcagagc ctgttacata tgcgctggca gtttcgcgac
    16861 ctcactttct ggcagctgcg ctttcgcacc aacactccta tcaccagcgg tggtagcgat
    16921 gacagtctgg aagccagtca catcagcgat ctgtttcttc gtcaatttga ccatttcgat
    16981 ctccgcgaca gtgaagtcag ttttctgacg ccatccggtc agcgcgccga gctggcgatc
    17041 ccacaactca cctggctgaa cgatccacgt cgacaccgtg cggaaggcct ggtaagcctc
    17101 tccagcctta ccggacagca cggcgtgatg caggtgcgca tggatttgcg cgatgatgag
    17161 gggttgttaa gcaatggtcg cgtctggcta caggcggatg acatcgacct gaagccgtgg
    17221 ctcggtaaat ggatgcagga caatattgct ctggaaacgg cacagttctc ccttgaaggc
    17281 tggatgacga tcgacaaagg cgatgtaacc ggcggtgacg tctggctgaa acagggcggt
    17341 gccagctggt tgggcgagaa gcaaacgcat acgctgtcgg tggataatct gaccgcgcat
    17401 attacgcgtg aaaatccggg ctggcagttc tctattcccg atacacggat cacgatggac
    17461 ggcaaaccct ggccgagcgg agcattgacg ctggcctgga taccggaaca ggacgttggc
    17521 ggcaaagaca ataaacgcag tgacgaactc cggattcgcg ctagtaatct ggagctggca
    17581 ggcctggagg gcatacgccc gctggccgcg aaactttcac ctgcactggg tgatgtttgg
    17641 cgctccacac aaccgagcgg caagattaac actctggcgc tggatatccc gcttcaggcg
    17701 gcaggcaaga cccgttttca ggcatcgtgg agcgatctgg cctggaagca atggaaatta
    17761 ttaccgggtg cggaacactt ctccgggacg ctttccggca gcgttgaaaa tggtttgctt
    17821 accgcgtcga tgaagcaggc aaagatgcct tacgaaacgg tattccgtgc gccactggaa
    17881 atcgccgacg gccaggcaac tataagctgg ctgaacaatg acaaaggttt ccagctggat
    17941 gggcgtaata ttgacgttaa agccaaagcc gtccatgcgc gcggcggttt tcgttacctg
    18001 caacctgcta acgatgaacc ctggctgggt attctggctg gcatcagtac cgatgatggt
    18061 tcacaagcct ggcgctattt cccggaaaac ttgatgggta aagacttggt tgattattta
    18121 agtggtgcga ttcagggcgg tgaagcggat aacgcgacgc tggtttatgg tggcaatccg
    18181 caactcttcc cctataaaca caacgaaggt cagtttgaag tgctggtgcc gctgcgcaac
    18241 gcgaagtttg ccttccagcc ggactggcct gcattaacta accttgatat tgaactggac
    18301 tttattaacg acggtttatg gatgaaaacc gatggcgtta atctgggcgg cgtgcgcgcg
    18361 agtaatctca ccgcagtgat ccctgactac tcaaaagaaa aactgctgat tgacgctgac
    18421 attaaaggtc cgggtaaagc cgttggccct tactttgatg agacaccgct gaaagattct
    18481 ctgggtgcga ccctgcaaga actccagctc gacggcgatg tgaatgctcg cttacatctt
    18541 gatatcccgc tgaacggcga actggtaacc gcgaaaggtg aagtgacgct gcgtaataac
    18601 agtctgttta tcaaaccact cgacagcatc ctgaaaaatt tgagcggtaa attcagcttt
    18661 atcaatggcg atctgcaaag tgaaccactg acagcaagct ggtttaatca gccgttgaac
    18721 gtggattttt ccaccaaaga aggggcaaaa gcctaccagg ttgcggtgaa tctcaacggt
    18781 aactggcaac cggcgaaaac cggcgttctg cctgaagcgg tgaacgaagc attgagtggc
    18841 agcgtggcgt gggatggtaa agtgggcatt gatctgcctt atcatgctgg cgcgacctat
    18901 aacgtagagc tgaacggcga tctgaataat gtgagtagtc acttaccttc accgttagcc
    18961 aaacctgcgg gtgaaccact agcggtaaac gttaaggttg atggcaatct caacagcttt
    19021 gaattaaccg gacaggctgg tgcggataac catttcaata gccgctggtt gcttggtcaa
    19081 aagctgacgc tcgatcgtgc tatttgggcg gcagacagta aaacgctccc gccgttgccg
    19141 gaacaaagtg gcgttgaact taatatgccg ccgatgaatg gtgccgagtg gctggccctg
    19201 ttccagaaag gcgctgcgga gagtgtcggt ggtgcagcga gtttcccaca acacataacg
    19261 ttacgtacgc ctatgttgtc actgggaaat cagcaatgga ataacctgag tattgtttcg
    19321 caaccgacgg caaatggcac cctggttgag gcgcaagggc gtgaaatcaa cgccacgcta
    19381 gcgatgcgta ataacgcgcc gtggctggcg aatatcaaat atctttatta caacccgagc
    19441 gtggcgaaaa ctcgtggtga ttcaacgccg tcatcacctt tcccgacaac ggagcgcatt
    19501 aacttccgtg gctggccgga cgcacaaata cgatgcacag agtgctggtt ctgggggcaa
    19561 aaattcggtc gcattgacag tgatatcacc atttctggcg atacgttaac gctgaccaat
    19621 ggactgattg atactggttt ctcgcggctt actgccgatg gtgaatgggt taataatccg
    19681 gggaatgaac gtacctcgct gaaaggaaaa ctgcgtgggc agaaaattga tgccgccgca
    19741 gaattttttg gtgtcacgac gcccatacgc cagtcgtcat ttaatgtgga ttacgattta
    19801 cactggcgca aagcaccctg gcagccagat gaagcgacgt tgaatggcat cattcatact
    19861 caactgggta aaggcgaaat taccgaaatc aataccggac atgccgggca attgctgcgc
    19921 ttattgagcg tagatgccct gatgcgtaag ctgcgttttg atttcagaga cacttttggc
    19981 gaagggttct attttgactc cattcgcagc accgcgtgga ttaaagacgg cgttatgcac
    20041 accgacgaca cgctggtgga tggcctggag gcggatatcg ccatgaaagg gtcggtaaat
    20101 ctggtacgcc gcgacctgaa tatggaagcg gttgtcgcac cagagatttc tgcgacggtg
    20161 ggcgtggctg cggcttttgc ggttaacccc attgttggcg cggcagtgtt tgccgccagt
    20221 aaagtgctgg ggccgctgtg gagcaaagtc tccattttgc gctatcacat ttcgggtccg
    20281 ctggacgatc cgcaaatcaa cgaagtgttg cgccaaccgc gtaaagaaaa agcgcaatga
    20341 tttgacgagg gcgcgtaatt gccccaatct catatgataa tcgttgccaa aggccaacga
    20401 gccggaacat aactgtaggc cggatagggc gttcacgccg catccggcag ccgtaaaaaa
    20461 tcctctactg cagtaactaa cgagtagcaa aaacgatgag tctgaacctg gtaagtgaac
    20521 aattgctagc ggcgaacggc ctgaaacatc aggacttgtt cgcgatcctc ggtcaactgg
    20581 ccgaacgtcg ccttgattat ggcgatctct attttcagtc gagctatcac gaatcctggg
    20641 ttttagaaga ccgcattatt aaagatggtt cttacaacat cgatcagggc gttggcgtgc
    20701 gtgcaatcag cggtgaaaaa accggatttg cttacgctga ccaaatcagc ctgctggcgc
    20761 tggaacagag tgcgcaagcg gcgcgcacca tcgtccgtga tagtggtgat ggcaaagtac
    20821 agacgctggg cgcggtagag catagcccgt tgtatacctc ggtagatccg ctgcaaagca
    20881 tgagccgtga agagaagctg gatatcctgc gtcgcgtcga taaggttgct cgcgaagcgg
    20941 acaagcgcgt acaggaagtg actgccagcc tcagtggtgt ctatgaatta attctggttg
    21001 cggccaccga cggcacgcta gcggcggatg tccgtccgct ggtgcgtctt tccgtgagcg
    21061 ttctggtcga agaagatggc aaacgcgaac gcggtgccag tggcggcggc ggtcgttttg
    21121 gttatgagtt cttccttgcc gatcttgacg gcgaagtccg tgcggatgca tgggcaaaag
    21181 aagcagtgcg tatggcgctg gtcaatcttt ctgccgttgc tgcaccagcg ggcaccatgc
    21241 cggtagtact tggcgcaggt tggccgggcg tgctgttgca tgaagcggta ggtcacggtc
    21301 tggaaggcga cttcaaccgc cgtggcactt cagtatttag tggacaggtc ggggagctgg
    21361 tggcttcaga actgtgtacc gtggttgatg acggcacgat ggtcgatcgc cgtggttcgg
    21421 tggcgattga tgatgaaggt acgccaggcc agtacaacgt gctgattgag aacggcattc
    21481 tgaaaggcta catgcaggat aaactcaacg cgcgtttgat ggggatgacg ccgactggca
    21541 acggtcgccg tgaatcctac gcccatctgc ccatgccgcg tatgaccaac acctatatgc
    21601 tgccgggtaa atcgaccccg caagaaatta ttgaatccgt tgagtacggt atctatgcac
    21661 cgaactttgg tggcggtcag gtggatatca cctccggcaa attcgttttc tccacttcag
    21721 aagcatatct gattgaaaac ggtaaagtaa cgaagccggt gaaaggcgca acgttgattg
    21781 gttccggtat cgaaaccatg cagcagattt cgatggttgg caacgacctg aaactggata
    21841 acggcgtggg tgtctgcggt aaagaagggc aaagtttgcc ggttggcgtg ggccagccaa
    21901 cgttgaaagt cgataacctg actgttggcg gtactgcgta ataatttcat tattttcaga
    21961 aggataatta atctttctac gtgcacgaac ggtcccctcg ctcctctggg gttagggtga
    22021 ggggaacccg ttggcacagg tttgtagaac gtaacagtac aatatgaatt acttctcttt
    22081 cccgcgcccg tgcatctcct gaaacaattt accgacctca acaaaataat ccgtcagcga
    22141 gttgatcacg acctgtacct tcagcggcag cttatctttt tcggtatata acgcataaac
    22201 cgggcgtgga tctgactgat aacgcggcag caggatctcc agctccccac gattgatctc
    22261 gttgatcacc cacatcagcg gcacgtaggc gatcccggca cccgccgtta gccagcgcac
    22321 cagcgtcatc ggatcattag tcacaaatct cccttgcggg atcaggcgag tcgagatccc
    22381 ttccggtgca atcagttcaa attcattgtc gggccgcacg ctgtattcaa gccatgaatg
    22441 gctacttaaa tcggcgggtt tttccggtat gccgtattgt gtgagatagc ttttcgcggc
    22501 gcacaccacc attggcatcg cgccaagacg gcgggaaaac aggctggaat cctgcaacgc
    22561 gccgacgcgg atcaccacat ccagaccgtc ggcaatcagg tcgggggctg gaattccggt
    22621 aaccagattg gcgctcaaac ctgggtattc tttcagcatt ttggctgtca gcccggcgag
    22681 aacattttgt gccatagttg aagaacagcc aatgcgtagc gtcccgatgg gggtgttatt
    22741 gaaggcatac agttgctcat gaacatcctg cacttcatga agcatacgac ggcagccctg
    22801 gtagtaaatt ctaccggctt cggtcaggcc aatgctgcgt gtgctacggt ttaacagctt
    22861 tacctgcaac tcatcttcca gttttgacac cgtctgactg atggacgaaa cgctcatctg
    22921 tagctgtctg gcggcggcgg taaaagagcc aaattcaact actttggcaa acaccgacat
    22981 gcgttttagt cgttccatta ttcactctga cttaaaagtg atttagatca cataatatag
    23041 ataacagcat aacagttacg ctaatatatt aaatatcaat ctacagcaat gttgctctcg
    23101 cccggctttc catgcccttc tctgcggcga cagatgctga aaataataac gcctgctctc
    23161 tctatacaac caaggtcaac atgagtctgt ttcccgttat cgtggtgttt gggctgtcct
    23221 tcccaccgat attttttgaa ttgcttttat cactggcgat tttctggctg gtgcgccggg
    23281 tacttgtgcc aacaggtatc tacgactttg tctggcatcc ggcgttgttc aacaccgcgc
    23341 tctattgctg cttgttttat ttgatatcgc gactgttcgt ttgaggttga agtgaaaaca
    23401 ctaataagaa aattctcccg tacggccatc acggtcgtat tagtcattct ggccttcatc
    23461 gcaattttta atgcctgggt ctattacacc gaatccccct ggacgcgtga cgcgcgcttt
    23521 agcgctgacg tcgttgcgat cgcgccggac gtttctggac tcattaccca ggtgaatgtt
    23581 catgataacc agctggtgaa aaaaggacag atactgttca ccatcgacca gccgcgctat
    23641 caaaaggcgc ttgaggaagc gcaagccgat gttgcttatt atcaggtact ggcacaggag
    23701 aaacgccagg aggccggacg tcgtaaccgt ctcggtgtgc aggcgatgtc tcgcgaagag
    23761 atcgaccagg ctaacaacgt actacaaacg gttctgcatc agttagcgaa agcgcaggcg
    23821 acccgcgatc tggcaaaact ggatcttgaa cgcacggtga tccgagcgcc agcagatggc
    23881 tgggtgacca acctcaacgt ctataccggt gagtttatta ctcgaggatc aacggcggtt
    23941 gcgctggtga aacagaactc cttctatgta ctggcctata tggaagaaac taagctggaa
    24001 ggggtgcgtc cggggtatcg tgcagagatc acgccgcttg gcagtaacaa agtgctgaaa
    24061 gggactgttg atagtgttgc cgctggggtc accaacgcca gcagcacgcg tgacgacaaa
    24121 gggatggcga ccatcgactc tgaccttgaa tgggtgcgtt tggcgcaacg tgttccggtt
    24181 cgtattcgtc tcgacaacca gcaagagaac atctggcctg cgggcaccac tgctacagtg
    24241 gtggtcactg gcaaacaaga tcgcgacgaa agccaggatt cgttcttccg taaaatggcc
    24301 catcgcctgc gtgagtttgg ttaatcacga tgggtatttt ctccattgct aaccaacata
    24361 ttcgctttgc ggtaaaactg gcgaccgcca ttgtactggc gctgtttgtt ggctttcact
    24421 tccagctgga aacgccacgc tgggcggtac tgacagcggc gattgttgcc gccggtccgg
    24481 cctttgctgc gggaggtgaa ccgtattctg gcgctattcg ctatcgtggc tttttgcgca
    24541 tcatcggcac atttattggc tgtattgccg gactggtgat catcattgcg atgatccgcg
    24601 caccattatt gatgattctg gtgtgctgta tctgggccgg tttttgtacc tggatatcct
    24661 cgctggtacg aatagaaaac tcgtacgcgt gggggctggc cggttatacc gcgctgatca
    24721 ttgtgatcac cattcagccg gaaccattgc ttacgccgca gtttgccgtc gaacgttgta
    24781 gcgagatcgt tatcggtatt gtgtgtgcga ttatggcgga tttgctcttt tctccgcgat
    24841 cgatcaaaca agaagtagat cgagagctgg aaagtttgct ggtcgcgcaa tatcaattaa
    24901 tgcaactctg tatcaagcat ggcgatggtg aagttgtcga taaagcctgg ggcgacctgg
    24961 tgcgacgcac cacggcacta caaggcatgc gcagcaacct gaatatggaa tcttcccgct
    25021 gggcgcgggc caatcgacgt ttaaaagcga tcaatacgct atcgctgacg ctgattaccc
    25081 aatcctgcga aacttatctt attcagaata cgcgcccgga attgatcact gatactttcc
    25141 gcgaattttt tgacacgccg gtagaaaccg cgcaggacgt ccacaagcag ctcaaacgcc
    25201 tgcggagagt tatcgcctgg accggggaac gggaaacgcc tgtcaccatt tatagctggg
    25261 tcgcggcggc aacgcgttat cagcttctca agcgcggcgt tatcagtaac acaaaaatca
    25321 acgccaccga agaagagatc ctgcaaggcg aaccggaagt aaaagtagag tcagccgaac
    25381 gtcatcatgc aatggttaac ttctggcgaa ccacactttc ctgcattctg ggcacgcttt
    25441 tctggctgtg gacgggctgg acttccggca gtggtgcaat ggtgatgatt gcggtcgtga
    25501 cgtcactggc aatgcgtttg ccgaatccac gcatggtggc gatcgacttt atctatggga
    25561 cgctggccgc gctgccgtta gggctgctct actttttggt gattatccct aatacccaac
    25621 agagcatgtt gctgctgtgt attagcctgg cagtgctggg attcttcctc ggtatagaag
    25681 tacagaaacg gcgactgggc tcgatggggg cactggccag caccataaat attatcgtgc
    25741 tggataaccc gatgactttc catttcagtc agtttctcga cagcgcatta gggcaaatcg
    25801 tcggctgtgt gctcgcgttc accgttattt tgctggtgcg ggataaatcg cgcgacagga
    25861 ctggacgtgt actgcttaat cagtttgttt ctgccgctgt ttccgcgatg actaccaatg
    25921 tggcacgtcg taaagagaac cacctcccgg cactttatca gcagctgttt ttgctgatga
    25981 ataagttccc aggggatttg ccgaaatttc gcctggcgct gacgatgatt atcgcgcacc
    26041 agcgcctgcg tgatgcgccg atcccggtta acgaggattt atcggcgttt caccgacaaa
    26101 tgcgccgcac agcagaccat gtgatatctg cccgtagcga tgataaacgt cgtcggtact
    26161 ttggccagtt gctggaagaa ctggaaatct accaggaaaa gctacgcatc tggcaagcgc
    26221 caccgcaggt gacggaaccg gttcatcggc tggcggggat gctccataag tatcaacatg
    26281 cgttgaccga tagttaagtc aaaaccgacg ccaaaagcgt cggttttttc atggctatac
    26341 ttagcgatgc acggcagaac tcgccgcgaa acgtgacggt ggcaacagat gaatatttat
    26401 gcctttgatt ttgatgagat tgagagtcag gaggattttt atcgtgactt tagccaaacc
    26461 tttggtctgg ctaaagataa ggtacgcgat ctcgactcac tatgggatgt gttaatgaac
    26521 gatgtcctgc cgctaccact tgagattgaa tttgttcatc tgggagagaa aacgcgtcgc
    26581 cgttttggcg cgttaatatt gctgtttgat gaggcagagg aagagctgga agggcatttg
    26641 cgttttaatg ttcgtcatta gcgcaaaaaa aagcccacga accgggggca atatcgtcgg
    26701 acaaaacgat gagggtttat ttgtacagtt cagccgtagc gtgccaggtg tcaccgctac
    26761 gagcttcagt aatctggtag gccgttgcgc ctttctcttc cgcttttttg ttcagcattt
    26821 cacgtatatc cattggcgaa gacgccacac cacttacgga tacggtcccg attgcttcac
    26881 gattttgtgc ttgtgcagca tcaatggagt cggcagcgaa tgcaccgaaa gagagaacag
    26941 aaagtacgct taatgcagct acagtggttt tgattttcat gatttttacc tcgacataat
    27001 cttttagctg ggcctttgtt tcgtgaccca tatcacaaaa tcaagtatac actaattatt
    27061 gaactaatta ataccacact aactgtttca gttgatacta agtgttaaaa atgtgaattt
    27121 taataacaaa aaagtataaa aaacacggtt atttattatt aattatagta ttttcaatta
    27181 gataatgaat tttgcataat gtgctttttt taccgtatgt attcattttg tgaatgatgt
    27241 gtcgcggtaa aacgcactat tcgttaaatg tatcgcagga gaaagaaggg gaatgagagg
    27301 gataagcaac attttccccg ccgccagaag cgacggggca gagattaaag ctcctggtcg
    27361 aacagctcta aaatcgcttc gtacaggtct ttgacggtga aaccgttagc aggggtggta
    27421 aagatggtgt catcgccagc gatggtgccc agaatacctt ctgctttgcc cagtgagtcc
    27481 agcaggcgag caattaactg cgccgcgcca gggctggtat gaatcacgac aactgcatcg
    27541 ttgtagtcga tatccagcac cagattcttc aatggactgg aggtggttgg tacacccagt
    27601 tcagctggca ggcagtaaac catttccatt ttggcattgc gtgtacgtac agcaccaaac
    27661 ttggtcaaca tccgcgagac tttagactga ttaatattgt caaagccttg ctcctgcaac
    27721 gcggcgacga tttcgccctg ggagctaaat ttctcttctt taagtaatgc tttaaatgct
    27781 ttaactagtt cttcttgctt agccgagctt cgcataagtc acccgatatg gtggttgata
    27841 caacattatt gtgcatacag atgaattttt atgcaaacag tcagccctga agaaggctga
    27901 aataatgtta tgaaagagcg ggattttatc aaatttcgtt attgagaaac atgcctgcgt
    27961 cacggcatgc aaattctgtt taaaagtaaa ttaattgtta tcaaattgat gttgttttgg
    28021 ctgaacggta gggtatattg tcaccacctg ttggaatgtt gcgctaatgc ataagcgact
    28081 gttaattacg taagttaggt tcctgattac ggcaattaaa tgcataaacg ctaaacttgc
    28141 gtgactacac attcttgaga tgtggtcatt gtaaacggca attttgtgga ttaaggtcgc
    28201 ggcagcggag caacatatct tagtttatca atataataag gagtttagga tgaaagtcgc
    28261 agtcctcggc gctgctggcg gtattggcca ggcgcttgca ctactgttaa aaacccaact
    28321 gccttcaggt tcagaactct ctctgtatga tatcgctcca gtgactcccg gtgtggccgt
    28381 cgatctgagc catatcccta ctgctgtgaa aatcaaaggt ttttctggtg aagatgcgac
    28441 tccggcgctg gaaggcgcag atgtcgttct tatctctgca ggcgtagcgc gtaaaccggg
    28501 tatggatcgt tccgacctgt ttaacgttaa cgccggcatc gtgaaaaacc tggtacagca
    28561 agttgcgaaa aactgcccga aagcgtgcat tggtattatc actaacccgg ttaacaccac
    28621 agttgcaatt gctgctgaag tgctgaaaaa agccggtgtt tatgacaaaa acaaactgtt
    28681 cggcgttacc acgctggata tcattcgttc caacaccttt gttgcggaac tgaaaggcaa
    28741 acagccaggc gaagttgaag tgccggttat tggcggtcac tctggtgtta ccattctgcc
    28801 gctgctgtca caggttcctg gcgttagttt taccgagcag gaagtggctg atctgaccaa
    28861 acgtatccag aacgcgggta ctgaggtggt tgaagcgaaa gccggtggcg ggtctgcaac
    28921 cctgtctatg ggccaggcag ctgcacgttt tggtctgtct ctggttcgtg cactgcaggg
    28981 cgaacaaggc gttgtcgaat gtgcctacgt tgaaggcgac ggtcagtacg cccgtttctt
    29041 ctctcaaccg ctgctgctgg gtaaaaacgg cgtggaagag cgtaaatcta tcggtaccct
    29101 gagcgcattt gaacagaacg cgctggaagg tatgctggat acgctcaaga aagatatcgc
    29161 cctaggcaaa gagttcgtta ataagtaatt gattagcgaa taataaaaaa ccggagcaca
    29221 gactccggtt tttgttttga gcactcgact taattggttg ccggatattc ctgaatggtg
    29281 acctgcagcg ttaactgctt atcatcacgc atcactacaa ccgggatcac cgaaccaggg
    29341 cgaatttccg ccacctgatc catcgtctcc agagcagaga tggccggttt gttatccacc
    29401 gaaataatca gatcgttgac ctgaataccc gcattcgccg ctgggccgtc aggtgacact
    29461 tcattaacca cgatcccttg cagttgatct ataccaccgc tctgcgcgtg cagtggtgcg
    29521 atctcgcgtc cgccgatacc aatgtagccg cggatcacgc gaccatcgcg gatcagctta
    29581 tccataattt tggttgctaa ctggaaagga atcgcaaagc cgataccttc cggcgtttcg
    29641 ccatcgttac tcttatcaaa cgacagcgtg ttaatgccca tcagttctcc cagcgagttc
    29701 accagcgcgc cgccagagtt accgtggtta atggaagcat cggtttgtag gaagttttgc
    29761 cgcccggtcg ggttcagacc gattcgaccc gtggcactaa taatcccttg ggtaatggtc
    29821 tgcccgaggt tgtacgggtt accgatcgcc agtactacgt cgccaatgtg cggtacgcga
    29881 cgtgcattaa ttggaatggt aggtaaaccg ccagtggcat taattttaag taccgccaga
    29941 tcggttagag agtcagatcc caccagcaat gcttcaaata cacgtccatc ctgtaaggcg
    30001 acgatgatct gatcggcgtc gttgatgacg tgtttattgg tgatgatata accgcgttga
    30061 tccatgatta caccggatcc cagggtgcgg atctcaagct ggttgtgaga gttggtgttc
    30121 aaaccacggt tgtaaacgtt aaccaccgct ggcgcggcgc ggcgaaccgc cagattatag
    30181 ctggcaggcg tctcatcggt actgtcaaat tgcggagtgg aaagcgggtt aaggctgcgc
    30241 agcgaaggca tggcaaccag cagaatagcg ccgacaatta atccaatcgc aacggaacgt
    30301 aagagcttca caaacatgat ggaggcgtca ttaaaaaagg gaacggcagc agcataccac
    30361 gagttaaccg gacatcacac gtcagcctga tgcccggttt acgacattaa cgcatcagca
    30421 gatagatgct ttcattgccg cgtacaattt gcagggcgat gatggccggt tttgccgcca
    30481 gcactttacg catttcagca atcgagttca cccgatcgcg gttgacgcca atgatcacat
    30541 cgtctttttg caagccagcc tgagcagctg ggcttccttt gacaacttca tctatcttaa
    30601 tacctttgcc gccatctttt agctgaccat cgctcaacgt tgcaccttcc agcgctggcc
    30661 gacgaagagg tgctggtatc aagcgtcact tctacttcca gtggtttgcc gttacgcagc
    30721 aggccaagct tcactttcgt gcccggctct gtagtcgcga tacgagagcg caactcagca
    30781 aagctattca gcggtttgcc gttgaggctg gtaataatat cgcccgcttt gacgcccgct
    30841 ttcgctgagc cagaacttgg caacacttcg ctgacaaacg cgccacgctg cacgtcaagg
    30901 ttgaaggctt tggcgatatc ggcactcatc tcggtgcctt tgatgcctaa caaaccgcgt
    30961 ttgatttcac caaagtcgat aagctgctgc gccagtgttc gcgccatatt actggggatg
    31021 gcaaatccaa tcccgacgct cccgccgcca ggcgcaagga ttgcagtgtt gatgccaatt
    31081 aactcaccgt taaggtttaa tagtgcaccg ccggagttac cgcggttaat ggaagcatct
    31141 gtctggataa agttttccag accttcaaga ttcaacccgc tgcggcctaa tgcggaaaca
    31201 atgccagagg tggcggtttg cccaaggcca aatgggttac cgaccgctac ggcaaaatca
    31261 ccgacgcgca atttatcgga gtcggcaata gcgatttgcg ttaatttgct cgggttttga
    31321 atttgtaaca gggcgatatc gctctggtca tcgctaccaa tcagttttgc atcaaactcg
    31381 cgcccatcat tgagctgaat actgattttc tgtgcctgat taatcacatg gttgttggtc
    31441 agcacatagc ctttactggc gttgatgatg acaccggaac ctaaaccttc gaagggttgt
    31501 gcaggttgat ccggtaaatc atcaccaaaa aactttttga attcttccgg gattttctgt
    31561 ccctgactgg ccgttccttc cacccgtacg ctcaccactg ccggaagcac tttttccagc
    31621 attggagcca gactggggag aggggcctga tcggcaacct ggcctggaat cgacgcgacg
    31681 gcctgaaatg acgccgagag agttaacccg gcacttaacg ctaatgcact caacagctgg
    31741 gtttgttttt tcattattcc tgctctcgta cctgaatgat aagaaaagag attcaaaaca
    31801 gtttgatgtt attgaatttt cagacggcta acaatgagac accagattaa atgatagtcg
    31861 gggagggaga gaagaggagg gcgcaatggc tgcgcccgaa aaataaatta gtcgcgcttc
    31921 gcgccagtac gcagcaggcc ggatgcgcct tcagaatagt cgcgaggcac ctgcaccggt
    31981 gcctgatcgt tgctggcttc agactctgcc agacgattac ggaacgggtt tgcttcagca
    32041 gacagttccg gcagcaggct gctggagctt tttgccatgt gctgatacag ctggcgatag
    32101 tcgtgcgcca tggtatccag taattccgcg ctgcgggcaa agtggctaac cagctcttcg
    32161 cgatactcgt ccagttcagc tttattcttt tccagttcgt actgcaacgc ctgttgctgg
    32221 cgtagtttac gattaccaaa acgcatggcc acagcaccaa taatgatgcc gacgactaac
    32281 ccaattagcg catattccca ggtcatgaac atctcccgtt gtcttttgtt tccgtagggt
    32341 gttggcttca ggctccatgc ctgcggctga ttatgccact ataaccgtta attccacaga
    32401 agtggaatcc cgactgcata tcgcgtagtg tagaacggcc tttttttcgt caaccgtgaa
    32461 caacagcgca ccgattattc aaggaataac aataagatca tgcaaagcgt taccccaaca
    32521 tcgcaatacc tgaaggcgct caatgaaggc agccatcaac ccgacgacgt tcaaaaagag
    32581 gccgtcagcc gcctggaaat tatttatcaa gaactcatca atagcaggcc accagccccc
    32641 aggacgagtg ggctaatggc gcgggtcggt aagctgtggg gtaaacgcga agacacaaag
    32701 catacgccag tgcgtggctt atatatgtgg ggcggtgtag gacgcgggaa aacctggctg
    32761 atggaccttt tctatcaaag cctgccggga gagcggaaac agcgcctgca ctttcaccgt
    32821 tttatgctgc gggtgcacga agagctaact gccttacagg ggcagaccga tccgctggaa
    32881 attattgccg atcgctttaa agccgaaact gacgtgctct gttttgacga attttttgtt
    32941 tctgatatta ccgacgccat gctacttggc ggtctgatga aagccctgtt cacccgcggt
    33001 attaccctgg tagcgacgtc aaatattccg ccggacgaac tttatcgaaa tggcctgcaa
    33061 cgtgcgcgtt ttctgcctgc aatcgatgcc attaaacagc attgtgatgt aatgaacgtg
    33121 gacgctggtg ttgattatcg actgcgtaca ctcactcagg cgcatctgtg gctttcgcca
    33181 ctcaacgatg aaacccgggc gcagatggat aaactatggt tggcgctggc gggggtgaaa
    33241 cgagaaaatt caccgacgtt agaaatcaac catcggccat tggcgacaat gggcgtcgag
    33301 aaccagacgc tggcggtttc ttttactacg ctgtgcgtcg acgcccgcag tcagcatgac
    33361 tatattgcgc tctcacgcct gtttcacaca gtaatgttgt ttgatgtgcc agtcatgacg
    33421 cggttgatgg agagcgaagc gcggcgcttt attgcgctgg tggatgagtt ttacgagcgc
    33481 catgtcaaat tagtggtgag tgcagaagtg ccgctgtatg aaatttatca gggtgagcgg
    33541 ctgaagtttg agttccagcg ttgcctgtca cgtctgcaag agatgcaaag cgaagagtat
    33601 ctgaagcgcg agcatttggc gggttaaaac ctgccacaaa tcacaaaaag gggtcgatct
    33661 ttgaccccga cttctctata atcctgcgac cccacgttac aagaaagttt ttttccccaa
    33721 aactttttgt gtgctggcat aggctattcg aaggggtagg tttgccggac tttgtcgtgt
    33781 gaacctcaac aattgaagac gtttgggtgt tcaccaacgt gtaactattt attgggtaag
    33841 cttttaatga aaacttttac agctaaacca gaaaccgtaa aacgcgactg gtatgttgtt
    33901 gacgcgaccg gtaaaactct gggccgtctg gctactgaac tggctcgtcg cctgcgcggt
    33961 aagcacaaag cggaatatac tccgcacgta gataccggtg attacatcat cgttctgaac
    34021 gctgacaaag ttgctgtaac cggcaacaag cgtactgaca aagtgtacta tcaccacacc
    34081 ggccacatcg gtggtatcaa acaagcgacc tttgaagaga tgattgctcg ccgtcctgag
    34141 cgtgtgattg aaatcgcggt taaaggcatg ttgccaaaag gcccgctggg tcgtgctatg
    34201 ttccgtaaac tgaaagttta cgcgggtaac gagcacaacc acgcggcaca gcaaccgcaa
    34261 gttcttgaca tctaatcggg attataggca atggctgaaa atcaatacta cggcactggt
    34321 cgccgcaaaa gttccgcagc tcgcgttttc atcaaaccgg gcaacggtaa aatcgtaatc
    34381 aaccaacgtt ctctggaaca gtacttcggt cgtgaaactg cccgcatggt agttcgtcag
    34441 ccgctggaac tggtcgacat ggttgagaaa ctggacctgt acatcaccgt taaaggtggt
    34501 ggtatctctg gtcaggctgg tgcgatccgt cacggtatca cccgcgctct gatggaatac
    34561 gacgagtccc tgcgttctga actgcgtaaa gctggcttcg ttactcgtga cgctcgtcag
    34621 gttgaacgta agaaagtcgg tctgcgtaaa gcacgtcgtc gtccgcagtt ctccaaacgt
    34681 taattggctt ctgctacggc agaaaacaat ttgcgaaaaa acccgcttcg gcgggttttt
    34741 ttatggctaa attctgaatc agcgtaaaaa atgggaagtt gttttttgct gccgtctgaa
    34801 agacaggtaa aacaaaaacc acatcgccaa taagggtcta agtcaactat ttcagactaa
    34861 agcgcatctc tttttcccca tttccggcat ccactcacca caaaggtcgc aaaatctggt
    34921 aaactatcat ccaattttct gcccaaatgt cgggtattgc tcattttttg tttgattttc
    34981 gaacaaagag agtcgttcct tattgggtaa cacaacttct gactggccac ctggtggctg
    35041 gtagcagtaa aaattctgac tatacctgga ggttttcatg gctgtcgctg ccaacaaacg
    35101 ttcggtaatg acgctgtttt ccggtcctac tgacatctat agccatcagg tccgcattgt
    35161 gctggctgag aaaggtgtaa gtttcgagat cgaacacgtg gaaaaggaca atccgcctca
    35221 ggatctgatt gacctcaacc cgaatcagag cgttccgacc ctggtggatc gtgagctgac
    35281 cctgtgggaa tctcgcatca ttatggaata tctggatgag cgtttcccgc atccgccact
    35341 gatgcctgtt tacccggtag ctcgcggtga aagccgtctg tacatgcatc gcatcgaaaa
    35401 agactggtac acgctgatga acactatcat caacggttca gcttctgaag cagatgccgc
    35461 acgtaagcaa ctgcgcgaag aactgctggc gattgcgccg gtcttcggtc agaagccgta
    35521 cttcctgagc gatgagttca gcctggtcga ttgctacctt gctccgctgc tgtggcgtct
    35581 gccgcaactg ggcatcgagt tcagcggccc gggtgcgaaa gagctgaaag gctatatgac
    35641 ccgcgtcttt gagcgtgact ctttccttgc ttctttaact gaagcagaac gtgaaatgcg
    35701 tctgggccgg agttaatctg tatggatttg tcacagctaa caccacgtcg tccctatctg
    35761 ctgcgtgcat tctatgagtg gttgctggat aaccagctca cgccgcacct ggtggtggat
    35821 gtgacgctcc ctggcgtgca ggttcctatg gaatatgcgc gtgacgggca aatcgtactc
    35881 aacattgcgc cgcgtgctgt cggcaatctg gaactggcga atgatgaggt gtgctttaac
    35941 gcgcgctttg gtggcattcc gcgtcaggtt tctgtgccgc tggctgccgt gctggctatc
    36001 tacgcccgtg aaaatggcgc aggcacgatg tttgagcctg aagctgccta cgatgaagat
    36061 accagcatca tgaatgatga agaggcatcg gcagacaacg aaaccgttat gtcggttatt
    36121 gatggcgaca agccagatca cgatgatgac actcatcctg acgatgaacc tccgcagcca
    36181 ccacgcggtg gtcgaccggc attacgcgtt gtgaagtaat acaaaacagg cccaggcggc
    36241 ctgttttgtc tttttaatga cttaataaaa tagcgtgatt gtggcaatca tattcaccac
    36301 gaaaccgact gccatgggga tcgctgtccg cttcaccacc gcaaaaggcg aaacgcctgc
    36361 aattcctgac gtaacgacca ccaccgcagt aatcggcgaa accgcgcgcg ctagcgtcgt
    36421 ggcaaaatgc atcggcataa tcattacaac cgctggtaca tgtagtccgg ctgcgatatt
    36481 cggaataaga ctggcaaatg acataaacgg cgcattgcca gagcccatca caatggcaca
    36541 aatggcaatg actagcgcca taataatcat cacgccaata ccgcccagac cagaatgctc
    36601 cgcacccctg ataaccgcat caacagtgcc aatcgtcgtt aagcctttcg caaatatttc
    36661 acccgcgacc accagcgtta ccacgttgac aaactgcgta cccatgccgt caaaaaacgc
    36721 ctgcacatcg tccattgttt cgcgcaagtt atgcttgcgg aagaactcaa caaacatcgt
    36781 caccgtcaaa cttagtaaca tcaccaccac cagatgcagt tctgacttca tcagcccgac
    36841 gtgggcgagg aacagcgagc cgagcatcag gattaacggc atcacaggta aaatggcgta
    36901 atagagcggc gggacattat cgagagtttt ttgctctgcc tgttcgtgat tgatattttt
    36961 atcttttttg tcaaaagcgc gttgcacgaa aaagtgggag atcgccaccg agataatgac
    37021 gcaagaggcg accggaagct ggtagtggaa gaagtatgtg gcaattttca ttcccgcgac
    37081 ctgggctgca aaaatggagt tcgtttccag aatcccccac tcaatggaca tcgtggttgc
    37141 gataaccgcc accgcagaga ggcgacttac tcccagactc accagcgtcg gaaataaggt
    37201 gaccatcagc aacataccca gaccggaggc gctggtaata aactgcgcca tgatttggcc
    37261 gatgacgtaa gttgccgaca gaataatata cggcgagcga atgagtttta acgggcggct
    37321 tagcaagctc accatcgcgc aactggctcc tatgcgctcc atgtagcggg cataaccgcc
    37381 caccgccata atcgacagcc ccaatccggc aatgcggttg gacagcatgc gcaggatttc
    37441 attataaatg tcgacgatca agtagccgct gcttttggcg ggatcgagta caccaccgaa
    37501 ccctaaccag actccgcaca tcatcaggaa gacacctgcg gcagccagca ccacctgagg
    37561 tttgtagttt ttcaggatca aatagcccat cgtaattaat acgatgacag atataattat
    37621 gccgaacatt gttgggtaat gactccaact tactgatagt gttttatgtt cagataatgc
    37681 ccgatgacct tgtcatgcag ctccaccgat tttgagaacg acagtgactt ccgtcccagc
    37741 cttgccagat gttgtctcag attcagatta tgtcgctcaa tgcgctgagt
//
LOCUS       DAEEUE010000030        36833 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.030, whole genome shotgun sequence.
ACCESSION   DAEEUE010000030 DAEEUE010000000
VERSION     DAEEUE010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36833
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.030"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..247
                     /locus_tag="KLK84_001655"
     CDS             <1..247
                     /locus_tag="KLK84_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBE2009020.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(294..1052)
                     /gene="deoR"
                     /locus_tag="KLK84_001656"
     CDS             complement(294..1052)
                     /gene="deoR"
                     /locus_tag="KLK84_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HBE2009021.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="KLK84_001657"
     CDS             complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="KLK84_001657"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBE2009022.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1991..3217
                     /gene="mdfA"
                     /locus_tag="KLK84_001658"
     CDS             1991..3217
                     /gene="mdfA"
                     /locus_tag="KLK84_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HBE2009023.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3258..3542)
                     /locus_tag="KLK84_001659"
     CDS             complement(3258..3542)
                     /locus_tag="KLK84_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009024.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="KLK84_001660"
     CDS             complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="KLK84_001660"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HBE2009025.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4443..5651)
                     /locus_tag="KLK84_001661"
     CDS             complement(4443..5651)
                     /locus_tag="KLK84_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE2009026.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5735..6271
                     /gene="rcdA"
                     /locus_tag="KLK84_001662"
     CDS             5735..6271
                     /gene="rcdA"
                     /locus_tag="KLK84_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HBE2009027.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6446..8131)
                     /locus_tag="KLK84_001663"
     CDS             complement(6446..8131)
                     /locus_tag="KLK84_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HBE2009028.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8401..8778
                     /locus_tag="KLK84_001664"
     CDS             8401..8778
                     /locus_tag="KLK84_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HBE2009029.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="KLK84_001665"
     CDS             complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="KLK84_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HBE2009030.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9225..9512
                     /locus_tag="KLK84_001666"
     CDS             9225..9512
                     /locus_tag="KLK84_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HBE2009031.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9496..10218
                     /gene="nfsA"
                     /locus_tag="KLK84_001667"
     CDS             9496..10218
                     /gene="nfsA"
                     /locus_tag="KLK84_001667"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HBE2009032.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10279..11181
                     /gene="rimK"
                     /locus_tag="KLK84_001668"
     CDS             10279..11181
                     /gene="rimK"
                     /locus_tag="KLK84_001668"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HBE2009033.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11269..11745
                     /locus_tag="KLK84_001669"
     CDS             11269..11745
                     /locus_tag="KLK84_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HBE2009034.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12097..13209
                     /gene="potF"
                     /locus_tag="KLK84_001670"
     CDS             12097..13209
                     /gene="potF"
                     /locus_tag="KLK84_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HBE2009035.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13304..14437
                     /gene="potG"
                     /locus_tag="KLK84_001671"
     CDS             13304..14437
                     /gene="potG"
                     /locus_tag="KLK84_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HBE2009036.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14447..15400
                     /gene="potH"
                     /locus_tag="KLK84_001672"
     CDS             14447..15400
                     /gene="potH"
                     /locus_tag="KLK84_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HBE2009037.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDSLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15397..16242
                     /gene="potI"
                     /locus_tag="KLK84_001673"
     CDS             15397..16242
                     /gene="potI"
                     /locus_tag="KLK84_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HBE2009038.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16302..16790
                     /locus_tag="KLK84_001674"
     CDS             16302..16790
                     /locus_tag="KLK84_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HBE2009039.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16831..17958
                     /gene="rlmC"
                     /locus_tag="KLK84_001675"
     CDS             16831..17958
                     /gene="rlmC"
                     /locus_tag="KLK84_001675"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HBE2009040.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="KLK84_001676"
     CDS             complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="KLK84_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HBE2009041.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19156..19824)
                     /gene="artM"
                     /locus_tag="KLK84_001677"
     CDS             complement(19156..19824)
                     /gene="artM"
                     /locus_tag="KLK84_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HBE2009042.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="KLK84_001678"
     CDS             complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="KLK84_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HBE2009043.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="KLK84_001679"
     CDS             complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="KLK84_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBE2009044.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21296..22024)
                     /gene="artP"
                     /locus_tag="KLK84_001680"
     CDS             complement(21296..22024)
                     /gene="artP"
                     /locus_tag="KLK84_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HBE2009045.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22242..22757)
                     /locus_tag="KLK84_001681"
     CDS             complement(22242..22757)
                     /locus_tag="KLK84_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBE2009046.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23406..25175
                     /locus_tag="KLK84_001682"
     CDS             23406..25175
                     /locus_tag="KLK84_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBE2009047.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25386..25709
                     /locus_tag="KLK84_001683"
     CDS             25386..25709
                     /locus_tag="KLK84_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBE2009048.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25706..26536
                     /gene="amiD"
                     /locus_tag="KLK84_001684"
     CDS             25706..26536
                     /gene="amiD"
                     /locus_tag="KLK84_001684"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HBE2009049.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTSVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26533..27546)
                     /locus_tag="KLK84_001685"
     CDS             complement(26533..27546)
                     /locus_tag="KLK84_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBE2009050.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27645..29075)
                     /locus_tag="KLK84_001686"
     CDS             complement(27645..29075)
                     /locus_tag="KLK84_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HBE2009051.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="KLK84_001687"
     CDS             complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="KLK84_001687"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBE2009052.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30124..31842)
                     /gene="poxB"
                     /locus_tag="KLK84_001688"
     CDS             complement(30124..31842)
                     /gene="poxB"
                     /locus_tag="KLK84_001688"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HBE2009053.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31975..32943)
                     /gene="hcr"
                     /locus_tag="KLK84_001689"
     CDS             complement(31975..32943)
                     /gene="hcr"
                     /locus_tag="KLK84_001689"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HBE2009054.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32955..34607)
                     /gene="hcp"
                     /locus_tag="KLK84_001690"
                     /gene_synonym="priS"
     CDS             complement(32955..34607)
                     /gene="hcp"
                     /locus_tag="KLK84_001690"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBE2009055.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(34751..35650)
                     /gene="lysO"
                     /locus_tag="KLK84_001691"
     CDS             complement(34751..35650)
                     /gene="lysO"
                     /locus_tag="KLK84_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HBE2009056.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(36107..36802)
                     /gene="aqpZ"
                     /locus_tag="KLK84_001692"
     CDS             complement(36107..36802)
                     /gene="aqpZ"
                     /locus_tag="KLK84_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HBE2009057.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT         9213 a         9156 c         9442 g         9022 t
ORIGIN      
        1 cgtgaccata ccgcgtgggc agctgaaaaa cctgaaggcg agctacacgt taacggaacc
       61 gcagcttacc gcaccgctga aaaaaggtca ggttgtcggg accattgatt tccagcttaa
      121 cggtaaatcc attgagcagc gtccgctgat cgtgatggaa aatgtggaag agggcggatt
      181 ctttggtagg atgtgggatt tcgtgatgat gaaattccat cagtggttcg gcagctggtt
      241 ctcttaatct tctgataacc ggatggcgcg aaacgtcatc cggttatacg tcattaatac
      301 atcaacttaa tacggtgcgt ctgcgcgtac ttcacatact catcttccgg gcaacaatca
      361 ctcaccacaa tatcaaagcg tttcaggtca cccatgcgcg ccggacgcac cttgccaaat
      421 ttactgtggt cgacaaccag cacatgcttt tgcgccatcg acatggccca gtgttttacc
      481 ggcaactctt caagattaaa acaggtagcg cctttactga catgcacgcc cgccgcggaa
      541 taaaaagcga tatccgggca aaaattattc aacgtttgct gaaaatcgat gggtttgaaa
      601 atggcgttgc tggcgtgaaa ttcaccaccg cacagaaatg cgcggcaatg gggtttctct
      661 ttcagcgcca gaaaggtatt tagcgaataa caaacggcgg taaaagggat ttcattatca
      721 atcgcttcaa taatccacgg cgtggtggtg ccacagtcaa aaaagagggt ctgatcgggt
      781 tctaccagtg tcgcagccag ttttgccgcc cggcgttttt cttccaccag gcgggatttt
      841 tgatcgctta acaggtaatg gctggcactg cgcggttcca gaacaatata gccgccgagc
      901 aaaacgacgg gcgcactgtg gttgttcaga tcgcgacgaa tcgtcatctc cgaaacccca
      961 agcagggcgg cggcgtcttt aagatgtaac ttatcgctgc gttttaattc ttgcagcagc
     1021 tgcccgatac gctcttcgcg acgtgtttcc ataatccctc tgaatagtta ttgaagcgag
     1081 ccgctcaata ctacactttt tagcagagat cagtcacgca cccagccttt gcggatcggt
     1141 aatgcaaaac agacgcgata ccacgattgc agacgttgat agagtttatg ccccatcgct
     1201 tgccagataa tctgggcact aaggcagcca atcatacctg ccagcaatcc accgagcata
     1261 tccagcggcc agtggacgcc aagataaacg cgcgaccagg caatgacgac ggccagcacc
     1321 attaaaagtg agccggacca caggcgatgc cagcataaaa atgccagtgc aaaggtgaaa
     1381 atcaccgtac cgtgatcgct cgggaatgag tcatccgccg catgatgcag gaagttatag
     1441 ccgatatttt cgacaaaggg tcggtcgtgc ggaaaaagat gtcccatcgt ccaggacaca
     1501 aacaggctga ccgccagcgc gatagcgatt tttatcacca gttgccgttg tgctgtaagc
     1561 ccccacaacc aaagtaccgc ggccagcaac ggcaccacgg taatcaaatc tttagcaata
     1621 aaaatcgcca acgagatcat ccacggagcc gagtctggcg tcgcgttaat aagagagaat
     1681 agagagagat tcaaattttc cagcataact tcccgacgca aagtgattaa aagggagcca
     1741 atacaggcaa gtcgttgaga ataaagtgca ggttaaactg ggtaaagcgg catcgtctta
     1801 tttccctcaa gcggcctgtt tacggtgggt gattgtaacg ggcataggtt aaataaaact
     1861 taaagaaagc gtagctatac tcgcaataat gtaagaatgt gcttaaccgt ggtttcagct
     1921 acaaaattcg ctttctcgtt agctgcgctt ttattaaact ctgcgcgatt attattggcg
     1981 aagaaattgc atgcaaaata aattagcttc cggtgccagg cttggacgtc aggcgttact
     2041 tttccctctc tgtctggtgc tttacgaatt ttcaacctat atcggcaacg atatgatcca
     2101 acccggtatg ttggccgtgg tggaacaata tcaggcgggc attgattggg ttcctacttc
     2161 gatgaccgcg tatctggcgg gcgggatgtt tttacaatgg ctcctggggc cgctgtcgga
     2221 tcgtattggt cgccgtccgg tgatgctggc gggtgtggtg tggtttatcg tcacctgtct
     2281 ggcaatatta ctggcgcaaa acattgaaca attcaccctg ttgcgcttct tgcagggcat
     2341 aagcttctgt ttcattggcg ctgtgggata cgccgcaatt caggaatcct tcgaagaggc
     2401 ggtttgtatc aagatcaccg cgctgatggc gaacgtggcg ctgattgctc cgctacttgg
     2461 tccgctggtg ggcgcggcgt ggatccatgt gctgccttgg gaggggatgt ttgttttgtt
     2521 tgccgcattg gcagcgatct cctttttcgg tctgcaacga gctatgcctg aaaccgccat
     2581 gcgtataggc gagaaactgt cactgaaaga actcggtcgt gactataagc tggtgctgaa
     2641 gaacggccgc tttgtggcgg gggcgctggg attcgttagc ctgccattgc tggcgtggat
     2701 cgcccagtcg ccgattatca tcattaccgg ggagcagttg agcagctatg aatatggctt
     2761 gctgcaagtg cctattttcg gggcgttaat tgcgggtaac ttgctgttag cgcgtctgac
     2821 ctcgcgccgc accgtacgtt cgctgattat tatgggcggc tggccgatta tgattggtct
     2881 gttggtcgct gctgcggcaa cggttatctc atcgcacgcg tatttatgga tgaccgccgg
     2941 gttaagtatt tatgctttcg gtattggtct ggcgaatgcg ggactggtgc gattaaccct
     3001 gtttgccagc gatatgagta aaggtacggt ttctgcggcg atgggaatgc tgcaaatgct
     3061 gatctttacc gtcggtattg aaatcagcaa acatgcctgg ctgaacgggg gcaacggact
     3121 gtttaatctc ttcaaccttg tcaacggaat tttgtggctg tcgctgatgg ttatcttttt
     3181 aaaagataaa cagatgggaa attctcacga agggtaaaaa aatgcctgat tgatttgtgc
     3241 gatcaggcat tctcgaatta atggtgatgg tcgtcaatct ggtgttcgat aaccatccct
     3301 tcacctacgc tggcaagatg gcgaacataa ggatgcgggc ggtaagccgg agctggcgca
     3361 ggagccacat agacggtttg cggcacagtc gcgacgctga ccgcttgtgg aacgctgact
     3421 gaatcaggaa ccacaaccac tttgtatcca ctcggcacat cgacggtgac actttgcgcc
     3481 atcgccacac cagtaaagcc cattaaaagc gcgcccaaca gtagacaatt tttcataatc
     3541 atttcgcctg aaataatgca gcgggttaat ttccgcgaat tatgcagcct tcattcaggc
     3601 attgtgttgc cgggcgtgct gccgttttta ttggtcaaat ggcgcttcgt gtttaagaac
     3661 tttatcgatc acatccagta cgccttcacg gttattgtag cctgcccggt attttgccgc
     3721 tgcgacgact gcgctgccgg cattttccat tgcaaaacta aagcctgcct gacgcagcat
     3781 ctcaatatcg ttaccgccat cgccaaagac caccacttcg ctgtcgtcta ttccccataa
     3841 tttctgcagt tggcgaaggc cattggcttt atgtacgccg gggataatca gatcgatgct
     3901 gccgttgccg gtgtggactg gcaccataat atcgccgatg gcctcatgta atgctttttg
     3961 tacttgtgga atcagttcat cggaaagatt caggccaaac ttaaagaaaa tatcttctaa
     4021 gttgtcaaag ttatcgacgt attccagacg gtgataatac atttccgcca ccgttttcat
     4081 ggcatcgtca tattttttga gtgtataggc gctatttttt ccgcaggcaa taatttccac
     4141 ttccgggcgc gtcagcaaat gttccacgac agtagcaaac gcatccttcg acagctcgcc
     4201 attaaaaaca tctttgcctt cactcattac ccagccgccg ttttcagcca caaaggcaat
     4261 ttcattcgca atttcaggga agaaagagat caactgataa tattgattcc cgctggcgac
     4321 cacaaagcga atgccttgtg ctttcatttg ctgatactga gccataaacc gctcacggtt
     4381 ataggttttt tgatcgctta agaaagtacc atccatgtct accgcaatta atttaatgct
     4441 catcaactat tctccatcgc cgtctgcgtt ttggtatcgg gtttggcgac ggctttcgcg
     4501 acaatagccg cgagaataac cagcgccagt acaaccagca ttgcactacg taatccataa
     4561 tgttcgccga gatagcccag cagcggcggc ccgacgagga aagccagata accggtcgtt
     4621 gctaccacac tgacgcgggt tggtgcatcg gggccggtat cgctggcggc agaaatggtc
     4681 agcgggaagc ccagcgaggc acccagtccc caaagtacaa cagacactcc tgcgacccag
     4741 gcgctatcga caaaaataat cagcccaata cccaacgccc ccattagtgc actggcccga
     4801 accacggcaa cgcgactgta actgtcgatg aaccaaccgc cggtgaagcg tccaacggtc
     4861 atccccaggg taaaaccggc ataaatcagt gagccggaag tggggctaaa accgtgacca
     4921 tcaaccatta ataagggtaa ccagtcgttg gcagaacctt cggcaaaggc catcgccagc
     4981 actacaacac caatcaacag caactggata tcgcgataaa aaggaatgcc tttttcgcca
     5041 tgctgggtgc catcggcagc atttttgccc gtaccgtcag ggattgcctg aatggcgata
     5101 taaataggcg cgatacctac cagcgccgcc aataaaatgt gcaccgttgc cggaacgcca
     5161 aaggccgtca gtgccatccc gacaccagcg cctgccagcg tgcccaggct ataaaaaccg
     5221 tgcatcatcg gcaaaaccgt tttattcatt tctcgctcaa cggcggcacc ttcaacgttt
     5281 atcgccactt ccgcagaacc aaaacttgcc ccaaagacgc cgagaccaac ggcaaagagc
     5341 aggggcgatg tcagccagag tgccagactt aatatcatca tcccgatcaa tgcgcaggac
     5401 atcgtgacca ggatgacatt acgtgtccca aagcgtttca ctaaccacgc cgagcagaga
     5461 ataccgctca tcgaaccgat cgacagacca aagagaacac cgcccatttc agcgatcgag
     5521 acagagagaa tatcgcggat agcaggcgta cgggttgccc aggacgccat taacaggcct
     5581 ggcaaaaaga agaacataaa cagcgcccag gttcggcgtt tcaatgcatt acgtgaagaa
     5641 tttacggtca tagatcacgt caaaataaga agagggaaga caacattagc aaggttgtgt
     5701 acatttgtac acaattgcag acagaggaaa tgacatgcgt cgcgctaacg atccgcaacg
     5761 gcgagaaaaa attatccagg ccacgctgga ggcggtgaaa ctttacggaa tacatgctgt
     5821 tacgcaccgc aaaattgcta cccttgccgg ggtaccgttg ggatcgatga cctactattt
     5881 ttcaggaatt gatgagttgt tgctggaggc gttcagcagt tttactgaga ttatgtcccg
     5941 gcaatatcag gcatttttta gcgatgttag tgatgctcaa ggcgcatgcc aggctatcac
     6001 cgatatgatc tacagctcac aggttgcaac gccggataat atggagctga tgtaccagct
     6061 ctacgcgctg gctagccgaa aaccgctatt aaaaacggta atgcaaaact ggatgcagcg
     6121 cagtcagcaa acgctcgaac aatggtttga acccggaacc gcccgcgcgc ttgatgcgtt
     6181 tattgagggg atgacgctgc attttgtcac cgaccgtaag ccgctatcgc gcgaggagat
     6241 tttgaggatg gttgagaggg ttgcagggta gtagataaat tttagataac aaaaaaccca
     6301 tcaaccttga accgaaatgg cggggttgat gggctccaca aaatggggac atcaaagaaa
     6361 agcagtggca ctaattaaga ctgatgccct gcggaaaagt tctgcggttg tgcaaaaaaa
     6421 tttcattttc agggcaactt cagttttatc ctaatcctgg ccataccatg acgatgattg
     6481 tccctgccag cgtcagcagg acgttggcga ttgcgtaggt gcccgcatag cccagcgcag
     6541 ggatgttact gcgagctgta tcactgatga tctccattgc cggcgcgcag gtgcgtgcgc
     6601 ccatcattgc gccgaacaac agtgcgcggt tcattcgcaa tacataagca ccgaacaaga
     6661 aacagataac cacgggcaca agactgacaa ttaatccggc aatcaacatc tgaccgccaa
     6721 tcgcgcccag gccgttatta ataccgctac cggcgctcag accaacgcct gccataaaca
     6781 ccatcaagcc gaactctttc accatgctta atgcaccttg cggaatgtaa ccgaaggtcg
     6841 ggtggttagc acgcataaag cccagcataa ttccggcgaa taacaacccg gcagcgttcc
     6901 ccatgccgaa actgaatgtg ctgaactgga aggtgatcat cccgatcatc agcccaataa
     6961 caaagaaggc gcaaaatgcc agcaggtcag tgacctggct gtgaatcgag ataaagccga
     7021 tgcgatcggc gatggttttt acgcgacggg catcaccgct gacttgtaaa acgtcacctt
     7081 tgttaagcac gacgttgtca tctatcggca tctcaatctg gctacgaatg acgcggttaa
     7141 ggaagcaacc gtgatcggtc aacttcagtt gtgcgagacg tttacctaca gcgttatggt
     7201 ttttaacgac cacttcttca gtgacgatac gcatgtcgag aaggtcacga tcgaaaactt
     7261 ctttaccgtt acggaagctg ggatcgagtc gggcatgggc gtcgggatag cctaccaacg
     7321 ctatttcatc gcccatttgt agcacggcat caccgtctgg atttgccaga atcccgttac
     7381 gtcgaatacg ttcaatgtag cagccggttt gtcgataaat acccagttca cgcagatttt
     7441 tgccgtcggt ccaggccacc agctccgggc cgacgcgata ggcgcggatc accggtaaat
     7501 aaaccttacg gttggcatca gtgtccaggc cacgttcgcg ggcgatttgc tgggcgctgg
     7561 tctgtaagtc ctgatgctgc aatttcggca agtaacgcgc accaacaatc aaactcacca
     7621 gaccgattaa ataggttaag gcatacccga ggctcagatt atccagtgcc agtgagagct
     7681 gcctgctttc catgccggaa tgatgcagtg tatcgccagc accgaccaga accggtgtcg
     7741 acgtcataga gcctgctaac ataccggccg tcaggccaat atcccagcca aacagcttac
     7801 ctaaccctaa ggcgatcacc agcgcactgc caaccatcac cagtgctaac attaggtaat
     7861 ttttcccatc acgaaaaaaa atggaaaaaa agttcggtcc ggcttcgacc ccgacgcaga
     7921 aaataaacag cataaagcca agattaagcg catcggtgtt aatgctgaaa tgttgttggc
     7981 ctaataacag cgatacgact aaaacgccaa tggaattacc cagttggatc gaaccaagtc
     8041 gtaactttcc gagacatagc ccaagcgcga ggaccacaaa taataacaga atgtaattcc
     8101 catttaacaa ttcggcgacg tttatattca cggaggctaa cttcttgttt actagtaagc
     8161 tgttgaaaga aatggtaatt tacgataatg tgttttacca gaattcaggg cgcagattca
     8221 ttcagcgcac ctaaacgata gtaaagtaac aatatatttt actagtgtaa tcacattagg
     8281 tatcaacggc tatatgaatt gcgttggcct atattagcat ggaatgcgaa gcggctttat
     8341 cttactgaac gccacactgg cgaaaaatgt gttcgataga cgcagtgtca ggaggaacga
     8401 gtgaaacata aacaacgttg ggcgggggca atctgctgtt ttgtcctctt cattgtggtg
     8461 tgcctttttc tggcgacgca catgaaaggc gcttttcggg ctgccgggca tcctgaaatc
     8521 ggcttgctgt ttttcattct tcctggagca gttgccagtt tcttttcaca gcgtagagaa
     8581 gtcctgaaac ctctatttgg cgcaatgctg gcggcaccct gttcaatgct cattatgcgg
     8641 ctgttttttt caccgacgcg ctcattctgg caagagctgg catggttact aagcgcggtg
     8701 ttctggtgtg cgctgggggc actgtgtttc ttatttatca gtagtttgtt taaaccacag
     8761 cacagaaaaa atcagtaaag ccctcaacgc gagggcttgt cagacgatca ggcgtccaga
     8821 ttttctttca cccatgcagc aaaatcggta tagccgccga tatgttgctg atcgacaaaa
     8881 atctgcggca cggtttctac gggtttacct gccttttgtt gtagatcttc tttagtgatc
     8941 ccttccgcac gaatatctac atactgatac tgaaaatcat cgcgttcatt gctcaatttc
     9001 tcagccagat cttttgcacg cacacagtaa gggcaacccg aacgaccaaa aataacggtt
     9061 tgcattattt ctctcctcat agatttatgc ctgtaatgat cacgctaaaa tgtattcgct
     9121 gaaagtaggt ttaacctgtt gcattaattg ctaaaagcta taactgttaa acacaataca
     9181 gtgaaaagtt ttagactgaa ggctcacttt gcagagggaa gcgtatgcgc gcgatcggta
     9241 aattgcctaa aggcgtgttg atactggaat ttatcggaat gatgctactg gcggtggcgc
     9301 tgctgtcggt aagcgactcc ctgtcgctgc ctgagccatt ttctcggcca gaagtgcaga
     9361 ttctgatgat ttttctcggt gttttgctca tgcttcccgc tgcggtggtg gttattcttc
     9421 aggtggcaaa acgtcttgcc ccacagctga tgaaccgtcc accgcaatat tcacgttcag
     9481 aaagagaaaa agataatgac gccaaccatt gaacttattt gtggccatcg ctccattcgc
     9541 catttcactg atgaacccat ttccgaagcg cagtgtgagg cgattattaa cagcgcccgt
     9601 gcgacgtcca gttccagttt tttgcagtgt agtagcatta ttcgcattac cgacaaagca
     9661 ttacgtgaag aactggtgac gctgaccggc gggcaaaaac acgtagcgca agcggcggag
     9721 ttctgggtgt tctgtgccga ctttaaccgc catttacaga tctgtccgga tgctcagctc
     9781 ggcctggcgg aacaactgtt gctcggtgtc gttgatacgg caatgatggc gcagaatgca
     9841 ttaaccgcag cggaatcgct gggattgggc ggggtatata tcggcggcct gcgcaataat
     9901 attgaagcgg tgacgaaact gctgaaatta ccgcagcatg ttctgccgct gtttgggctg
     9961 tgccttggct ggcctgcgga taatccggat ctcaagccgc gtttaccggc ctccattttg
    10021 gtgcatgaaa acagctatca accgctggat aaagacgcac tggcgcagta tgacgagcaa
    10081 ctggcggaat attacctcac ccgtggcagc aataatcgct gggatacctg gagcgatcat
    10141 atccgccgaa caatcattaa agaaagccgc ccatttattc tggattattt gcacaaacag
    10201 ggttgggcga cgcgctaaaa ccgctacgtc gatgtatgat acgcgggctt ttgaccaggt
    10261 ctgacagaga ggtgcagggt gaaaattgcc atattgtccc gggatggaac gctctattcg
    10321 tgtaagcggc tgcgtgaagc cgctatacag cgcggtcacc tggttgaaat tcttgatccg
    10381 ctttcttgct acatgaacat aaatcctgcg gcgtcttcta ttcactacaa aggccgcaag
    10441 ttaccccatt ttgacgcagt gatcccgcgt attggcaccg ccattacctt ttatgggacg
    10501 gcggcactgc gccagttcga gatgctgggg agctatccgc tcaatgagtc ggtcgccatt
    10561 gcccgggcgc gtgacaaatt gcgttccatg caactgctgg cgcgtcaggg catcgacctg
    10621 cctgtcacgg gcattgcgca ttcgccggat gataccagcg atttaatcga catggtcggt
    10681 ggtgcgccgc tggtggtcaa gttggttgaa ggcacgcagg gaattggcgt cgtgctggcg
    10741 gagactcgtc aggcggcgga aagcgtgatt gacgctttcc gcggtctgaa cgcgcatatt
    10801 ctggtgcagg aatatatcaa agaggcgcaa gggtgcgata tccgctgtct ggttgttggt
    10861 gatgaagtgg tcgctgcgat tgaacggcgg gcgaaagagg gctattttcg ttccaatttg
    10921 catcgtggcg gcgcggcaag cgtcgccagt atcacaccac aggagcgtga aatcgcgata
    10981 aaagccgcgc gaacgatggc gctggacgtt gctggtgtgg atattctgcg tgctaatcgc
    11041 gggccgttgg tgatggaagt gaatgcgtcg ccggggctgg aaggaataga aaaaaccacc
    11101 ggtatcgata tcgcgggtaa aatgatccgc tggatcgaac gccacgctac gacagaatat
    11161 tgcctgaaaa cgggtggtta gtcgcaatca cattactgat catggttttg cctgcgcttt
    11221 ttgcgtaagc tgtgccggtc tttttatcga aagaggttgt acaaaattat gacatcgctg
    11281 gtcgttcctg gtctggatac gctgcgtcaa tggctcgatg acctggggat gagttttttt
    11341 gaatgtgata actgtcaggc tctgcatctg ccccatatgc agaatttcga cggtgtcttt
    11401 gatgccaaaa tcgatctgat cgataacacg atcctgtttt ctgccatggc ggaagtccga
    11461 ccttcagccg tattgccgct ggcggcggat ttatctgcca tcaatgccag ttcgctgacc
    11521 gtgaaagcat ttcttgatat gcaggatgat aatctgccaa agctggtggt ttgccagtct
    11581 ttatccgtta tgcagggcgt aacctatgag cagtttgcat ggttcgtgcg tcagagcgaa
    11641 gagcagattt cgatggtcat tcttgaagct aatgcccatc aactgctgtt accgactgac
    11701 gatgaagggc aaaacaacgt taccgaaaac tattttctcc actgataatt cctttcgagc
    11761 acgcagtcgc tgctgcagtg actgcgcgct gcaaaattat ctgctgtttt taaccttttc
    11821 ttaaagatta tttcacttct cttgtgtcga tttggcttta tcacatagag caaatacgca
    11881 taaaaatttg ttaaataccg ttttttaatc cgagctatag tctcaaaccc tggctaaagt
    11941 tattcttgcg atgcttctat atagcgagca gtgctggccg ggagagagtt ctcttttctt
    12001 acaccgcgcc gataaaaaaa tatgcacgtt tattgcatat ctttcagtgt gacaactttt
    12061 gttcgtttgt taacgaactt tcagaaggaa agagatgtga ccgccttaaa taaaaaatgg
    12121 ctatcgggtc tggttgcggg tgctctgatg gccgtctctg tcggcacgct cgcggctgaa
    12181 caaaaaacac tccacattta taactggtct gattatatcg cccctgacac ggtggccaat
    12241 tttgaaaaag aaaccggtat taaagtcgtc tacgatgttt tcgactctaa cgaagtactg
    12301 gaaggcaaat taatggcggg tagcaccggc tttgacttgg tggttccatc tgccagcttt
    12361 ctagaacgcc agttgactgc gggagttttc cagccgctgg acaaaagcaa attgccggag
    12421 tggaagaatc tcgacccgga gctgctgaag ctggtcgcca aacacgatcc cgacaataaa
    12481 tttgctatgc cctatatgtg ggcgacgacc gggattggct ataacgttga taaagttaaa
    12541 gctgtgctgg gcgaaaacgc gcccgtcgat agctgggact tgatcctcaa acctgaaaat
    12601 ctggaaaaac tgaaaagctg cggtgtctct ttcctggatg cgccagaaga agtttttgct
    12661 accgtgttga attatctcgg caaagatccc aacagcacta aagcggatga ttacaccgga
    12721 ccggcaaccg atctgctgtt aaagctgcgc ccgaacattc gttatttcca ttcatctcaa
    12781 tacattaacg acctggcaaa cggcgatatt tgcgtcgcta tcggctgggc aggtgatgtc
    12841 tggcaggcgt caaaccgcgc gaaggaagcg aagaatggag tgaatgtctc gttctcgatt
    12901 ccaaaagaag gggcgatggc gttctttgat gtattcgcca tgcctgcgga tgccaaaaac
    12961 aaagacgaag cctatcagtt cctgaattac ctgctgcgcc cggatgtagt agcgcatatt
    13021 tccgaccatg tgttctatgt caacgccaat aaagcagcca cgccgctggt gagtgcggaa
    13081 gtccgtgaga acccgggtat ttatccgcct gcggatgttc gtgcgaagct gttcactctg
    13141 aaagtgcagg acccgaaaat cgaccgtgtg cgcacccgcg cgtggaccaa agtgaagagc
    13201 ggaaaataat ccgcagcctt agatgccggg cgggcgcacc acacccgccg gcaattcgca
    13261 ccatcatggt gcgcttgcac acattcaatg ccggagagca gccgtgaatg acgctatccc
    13321 tcgcccgcag gcgaaaaccc gtaaggcgct gacgccgcta ttagaaatcc gcaacctcac
    13381 caaatcctac gatggtcaac atgcggtgga tgatgtcagc ctgaccatct acaaaggtga
    13441 aatcttcgcg ctgctgggcg catccggctg tggcaagtcc acgctgctgc gtatgctggc
    13501 aggtttcgaa caaccttctg ccggacagat aatgcttgat ggcgtcgatt tgtcacaggt
    13561 tccgccttac ctgcgcccca tcaatatgat gtttcagtct tacgcgctgt ttccgcatat
    13621 gaccgtggaa cagaacatcg cttttggcct gaaacaggac aaactaccga aagcggaaat
    13681 ttccagtcgg gtcaatgaga tgctcggcct ggtgcacatg caggagttcg ccaaacgtaa
    13741 accgcatcag ctttctggtg gtcagcgaca gcgtgtggcc ctggcccgaa gccttgccaa
    13801 gcgcccgaaa ctattactgc tcgacgagcc gatgggcgcg ctggataaaa agctgcgtga
    13861 ccggatgcag cttgaagtgg tggatattct ggagcgcgtc ggtgtgactt gtgtgatggt
    13921 cacccacgat caggaagagg cgatgaccat ggcggggcgc atcgccatta tgaatcgtgg
    13981 gaaatttgtc cagattggcg aaccggaaga gatctacgag catccgacta cccgctatag
    14041 cgccgaattt attggctcgg taaacgtctt tgaaggcgta ctcaaagagc gtcaggaaga
    14101 tggcctggtg cttgattcgc cggggctggt gcatccactg aaagtcgacg cggatgcctc
    14161 ggtggtcgat aacgtgccgg tacatgtggc gctgcgcccg gaaaaaatca tgctttgcga
    14221 agagccgccc gccaatggtt gtaacttcgc ggtgggggag gtgatacaca ttgcctatct
    14281 cggcgatctt tcggtgtatc acgttcgtct gaaaagtggg cagatgatta gtgcccagct
    14341 acaaaacgcc catcgtcatc gtaaagggtt accgacctgg ggcgacgaag tgcgtttgtg
    14401 ctgggaagtg gacagctgtg tggtgctgac ggtttaagga gcaaagatga atacacttga
    14461 acctgctgcc cagtcgaaac cgccgggcgg atttaagctg tggttgtcgc agctgcaaat
    14521 gaagcatggg cgcaaactgg tcattgcgtt gccatatatc tggttgatct tgctgtttct
    14581 gctgccattt ctgattgtct ttaaaataag cctggcggag atggcgcgcg ctattccacc
    14641 ttataccgaa ctgatggagt gggctgacgg gcaactgtcc atcactctta atctcggtaa
    14701 ttttctgcaa ctgaccgacg attcgctcta tttcgatgct tatctccagt cgctacaggt
    14761 ggctgcgatt tcgacatttt gctgtttact gatcggctat ccgctggcgt gggcggtggc
    14821 gcacagtaag ccttcgaccc gtaatatttt attactactg gtgatcctgc cgtcgtggac
    14881 ctcgtttctg atccgcgttt atgcttggat gggaatatta aaaaacaacg gtgtgctgaa
    14941 taattttctg ctgtggctgg gggttatcga tcaaccgctg accattcttc ataccaatct
    15001 ggcagtttat attggcattg tttacgccta cgtgccgttt atggtgctgc cgatttatac
    15061 cgcgttgatt cgtattgatt attcgctggt ggaagcagcg ctggatctcg gtgcacgacc
    15121 gctgaaaacg ttctttactg tgatcgtgcc gctgactaaa ggtgggatta ttgccggatc
    15181 gatgctggtg tttatcccgg ctgtgggcga gtttgtgatc ccggaactgc tcggtggccc
    15241 ggacagcatc atgatcgggc gcgtgctatg gcaagagttt ttcaataacc gcgactggcc
    15301 ggtggcctcg gcggtagcga tcatcatgtt gctgctgcta attgtgccga taatgtggtt
    15361 ccacaaacac cagcaaaaaa gcgtgggaga acacggatga ataatttacc ggtagttcgt
    15421 tcgccctggc ggattgtgat tttgctgctg ggctttacct ttctctacgc gccaatgctg
    15481 atgctggtta tctattcgtt taacagctcg aagctggtga cggtgtgggc cggctggtca
    15541 acgcgctggt atggtgagtt attgcgcgat gacgcgatga tgagtgcggt tggttcaagc
    15601 ctgacaattg ctgcctgtgc ggcaacggcg gcggcgatcc tcgggactat tgcggcggtg
    15661 gtgctggtgc gctttggcag gtttcgcgga tcaaatggct ttgcctttat gatcaccgcg
    15721 ccgctggtaa tgccagatgt catcacgggc ttgtcgctgt tgttattatt cgtcgcgctt
    15781 gctcatgcca ttggctggcc tgcggaccgc ggtatgctta ccatctggct ggcgcatgtc
    15841 actttctgta cggcttatgt ggcggtcgtt atttcgtcgc gtctgcggga actggatcgc
    15901 tcgatagaag aagccgcgat ggatctcggt gcgacgccgc tgaaagtgtt tttcgtcatt
    15961 acgctaccga tgatcatgcc cgcgatcatt tctggctggt tactggcttt tactttgtcg
    16021 cttgatgatc tggtgatcgc cagctttgtt tccgggccgg gagccaccac gttaccgatg
    16081 ctggtctttt ccagcgtgcg gatgggggtg aatccggaaa tcaacgccct ggcaacattg
    16141 atcctcggcg cggtcggaat tgtcggattt atcgcctggt atctgatggc gcgcgcagaa
    16201 aaacagcgga tacgcgacat ccaacgtgca agacgtggct gaagacacta aaatttgcca
    16261 acctggctac ataatgccgc gcatgtcgcg gcattgtttt catggaagac gaaacgttgg
    16321 gattttttaa gaaaacatct tcatctcatg ctcgcctgaa tgtgcctgcg ctggtgcagg
    16381 tggcggcgct cgccattatt atgatccgtg gcctcgacgt gctgatgatt ttcaatacgc
    16441 tgggcgtgcg cggtattggc gagttcattc atcgcagcgt acaaacctgg agtttaacgc
    16501 tggtcttttt aagcagtctg gtgctggttt tcattgagat ctggtgtgcg ttttcactgg
    16561 tgaaagggcg tcgctgggcg cgctggctat atctgctgac acaaatcacc gccgcaagtt
    16621 acttgtgggc ggcttcgctg gggtacggtt atccggagct gttcagcatt cccggtgaat
    16681 caaaacgtga aatcttccat agcctgatgc tgcagaagct gccggatatg ctcatcctga
    16741 tgctgctgtt cgttccctcg accagtcggc ggttcttcca gttgcaataa tgtgtataat
    16801 cgtcgcccct gatgatgtga aggtcaatgt atgcagtgcg cactttacga cgcgggtcgc
    16861 tgtcgttcct gtcagtggat aacgcagccg attccagagc aactctccgc taaaaccgcc
    16921 gatcttaaaa atctgctcgc cgattttccg gttgaggaat ggtgcgcgcc ggtgtcaggc
    16981 ccggaacaag ggtttcgtaa taaagccaaa atggtggtga gtggtagcgt tgaaaaaaca
    17041 ctgctcggta tgctgcatcg cgatggcacg ccggaagacc tttgtgactg cccgctttat
    17101 ccagcctcat ttgcgcccgt ttttgcggcg ctaaaaccgt ttatcgcccg agcggggtta
    17161 acgccctaca acgtggcgcg taagcgtggc gaactgaaat acattctgct gactgaaagc
    17221 cagagtgatg gcggcatgat gctgcgcttt gtactgcgtt ctgataccaa gctggcgcaa
    17281 ctgcgtaagg cgctgccgtg gttacacgaa caactaccgc agctgaaagt tattaccgtc
    17341 aatattcagc cggtacatat ggcgattatg gaaggggaga cggagatcta cctgaccgaa
    17401 caacaggcac tggcggagcg ttttaatgac gtaccgctgt ggatccgtcc gcaaagtttc
    17461 ttccagacta atccggcggt cgccagccag ttgtacgcca ccgcgcgcga ctgggtacga
    17521 cagctgccgg ttaaacatat gtgggatctg ttctgcggtg tggggggctt tggtttacac
    17581 tgcgcgacgc ctgacatgca gttaaccggg atcgaaattg cgccagaggc cattgcctgt
    17641 gcaaagcagt cagccgctga actgggctta actcgtttac aatttcaggc gctggactcc
    17701 actcagtttg ccactgctca gggggaggtg ccggagctgg tgctggttaa cccgccgcgc
    17761 cgcggcattg gtaaaccgct gtgtgattat ctttcaacga tggcaccgcg ttttattatc
    17821 tactccagct gtaacgccca aaccatggcg aaagatatcc gcgaactgcc aggttaccgc
    17881 atcgaacggg tacagctttt tgatatgttc ccgcacaccg cgcactatga agtgttgacg
    17941 ctgctggtga agcaataaaa aagccacagg tgcggcttca gattgctgat caggtgcgct
    18001 ttgttcatgc cggatgcggc gtgaacgcct tatccggcct acaaaatcat gctaaatcaa
    18061 tacattgcag agttgatgtg ggccggataa gcgcagcgca tcaggcaatc ttacttttgt
    18121 catcagtctc aattactgcg gaaaccactg gtcattgatt ttctgatatg taccgtcagc
    18181 tttaattgct gccagcgcgt tattcagttt ttccagcaga gctttgttat ccggacgtac
    18241 agcgataccc aggccggtgc cgaagtattg cggatcggtc actttttcag tcgcaacgcc
    18301 cagttgcgga ttggttttca gccattcgtt taccaccgcg gtgtcaccaa ataccccatc
    18361 aatacgacca tttttcagat cgataaaggc attctgataa ctgtcatagg agacagtttt
    18421 cacttccggg tgctgatcct gaatatattt ctggtgcgtc gtaccgtttt ccatcccaat
    18481 gcgtttgcct ttcaggtcgg caaacgtttt gtaggtatct tttttggaaa tcacgacggc
    18541 tgagttttca tagtagggtg tggtaaacga aacctgcttg ctacgctccg gggtgatatc
    18601 cataccggag attacggcgt catattttct gaatttcagt gacgggatca ggctgtcgaa
    18661 cgcgtgatta gtaaaagtac attctgcctg catttgtttg cacaaggctt ttgccagatc
    18721 gatatcaaag ccgacaatct cattattagc acctatagat tcaaagggtg gataggtggc
    18781 tgaaacgcca aaattgattt tctctgcggc agaagcaccg aaagtaaagg aagcaagtaa
    18841 agcggcaaga actaactttt tcatgatgga actcccgtct gtcaatctta tgatttttgg
    18901 ccgtgtctgc ggcatgggat aacaatgcca tcaagtgaat ttatatgcaa taaacatgat
    18961 taaataattt aaatgaaata aaaaaagacg gataacttag tgagttgtcc gtcttcatta
    19021 taagaattta tgcactatgt aggccggata aggcgtccac gccgcatccg gcacaggcac
    19081 cgtgctgatg tctgatgcga cgctggcgcg tcttatcaga cctacaaacc cccccggcga
    19141 atgtacgcag ctacattaat tccgccgttc gaatgccagt gctttgcgct cgatcagacg
    19201 catcatcagc gtcagcaggc cgttgacgac caggtaaata atccctgccg caccgaacac
    19261 cattacatcg taggtgcgtc cgtacaacaa ctggctgtat cccatcactt ccatcagcgt
    19321 aatggtgtaa gccagagagg tacttttgaa caccagcacc acttcgttgg aataagaaga
    19381 gagcgagcgt ttaaaggcat acggcagcag gatcgccagc gtatcttttt tgctcattcc
    19441 cagggcgcta caggactgcc actgaccttc tgggatcgca cgaattgcac cgtaaaacag
    19501 ctgcgtggta tacgccgcac tgttcagcga taacgcaatc agcgcacata accacggttc
    19561 tgacaacaaa tgccacagtg ccggatactc ctgcaacgtc ggaaattggc ccggcccgta
    19621 ataaatcagg aagatctgca ccagcagcgg cgtaccggta aacagcgtga tataaccccg
    19681 caccagccac accagcaccg gcgttttcag cgtcaggatg atggtaaaaa tcaatgccag
    19741 aatcagtgcc acaatcagcg aggcaacggt tagcgtcagg ctggtgtgca gccctttcat
    19801 cagctcgggt aaatactcaa acattagctg ggcctccgct caaaacgtgt cgcgcgcagg
    19861 tcaatgcgtt tgagaatgta ctgactgagc agggtgatca ccaggtaaat cgccgccgcc
    19921 acaatgtacc aggtaaatgg ttcctgggta cgagtagcaa tgcttttggt ttgtaacatt
    19981 aaatcattca cactaatcaa actgaccagc gcggtatctt tcagcagcac cagccactgg
    20041 ttaccgaggc caggcagcgc atgacgccac atctgcggca tcaccagacg gaaaaagata
    20101 gccgatttcg acagccccag cgcctgacca gattcccact ggcccaccgg tacagctttc
    20161 aacgcgccgc gcagcgtttg cgaggcatag gcggcataca gcagtgacag agcgatgaca
    20221 ccacaaagga acggactcac gtcgaagttc tcaatgtcca tctgcaccgg gatctgcacg
    20281 aacccaagat tgatagtgaa gccatccgaa agcgtcagca gtaactgcga ggagccaaaa
    20341 tagataaaca gcactaccag aatttctggc aggccacgca gaatggttac cagcgctgaa
    20401 cctgcccacg cgacaggacg ccatttcgct gactcccata ccgcaaagaa catcgccagc
    20461 gccagcccga caatcaatgc acaaacggca aggccgacgg tcatcccggc ggcgcttgct
    20521 aaaggaaaaa attcattcat caggaattac ttctggaacc atttgttgta gatggtttcg
    20581 taagtgccat ctttcttcac tttttccagc gcagtgttga gtttctgctg cagttcagtg
    20641 ttgccctgac gtaccgcgat gccgaggcca gtaccgaaat aatctttatc ggtcacttta
    20701 tcgcccactg ccgccagttt tgggttatct ttcagccact cagtgaccac tgcggtgtca
    20761 ccaaatacgc tgtcgatacg cccgttttgc agatccagtt ttgcgttctg gtagctgtca
    20821 tacggaacgg tggtgatttc cgggtgctta tccataatga acttctggtg tgtcgtcccg
    20881 ttctgaacgc cgactttttt gcctttcagc tgatcaacac tggtgtattt gccttgctga
    20941 cccacaaaca gggcagagtt gtcatagtac ggggtagtaa acagtacctg cttttcacgc
    21001 tccggagtga tatccatgcc cgccatcact gcttctacgc gacggaattt caggcttggg
    21061 atcaggctgt caaacgcctg gttagagaaa gtacaggttg catcaatctc tttacacagt
    21121 gcctgtgcca ggtcgacgtc aaaaccaacg atctggttgt ttgcatcaat cgattcaaac
    21181 ggaggatagg gggcttcggt agcaaaacga atggtttcgg cagctgtggc ggaaagacta
    21241 aaacctgcaa ttaacgcggc aatcagaact tttttcattg ttgttatccc gaatcttagt
    21301 gagagagata gtttttaaat gcttcggttt gcggctcggt aaagcagctc gcgtcgcctt
    21361 gttctacgat atgaccattt tccatataca ccactcggct ggcggtttta cgcgccactt
    21421 caacttcgtg ggtgacgatc acctgggtaa tattcgtttc tgccagctca cgaatgatgc
    21481 tgacgatttg tgccgtaatt tccgggtcca gtgcggcggt cggttcatcg aacagcagta
    21541 cctgcggttc catcatcaac gcacgggcaa tagcaacacg ctgctgctga ccaccagaaa
    21601 gatgcagcgg gtaacgatcg ctataaggtt tgagacgcag acgttccagc agtttttctg
    21661 cacgggccag cgcctgatct ttactcaacc ccagtacacg gcagggcgct tcaatcaggt
    21721 tttgctgcac ggtcagatgc ggccacaggt tgtattgctg aaacaccatg ccaacgttac
    21781 gacgcaaatc gcgaatcgct ttgtcagagg gtgttttggt gaaatcgaaa tggttgcctg
    21841 caatgttgag cgtaccggag cgcggcatct caagcagatt gagtacacgc agcagcgagc
    21901 ttttacccgc gccgctgggg ccaagtaaca ccagcgtttc gccctgtggg caatccagcg
    21961 tgatatcgaa cagcgcctga tgcgcgccgt agaagcaatt aatgccgttt aattgaatac
    22021 tcattgacac tcgtatactg gcagtctgat agctattgag gtcgaagata gtacctttga
    22081 cagaataatt atgcaatatt tatgctttaa aagttaaaag caaagcgcat tattcaataa
    22141 acatagcaca aaataacggg ggcggtggtc ggtgagcata aatgtcggca ttcctcacga
    22201 aatgccggat aatttacggg gtttattggt tgatcaaggc gttagcgatt ctcgatggac
    22261 tgacggagcg tacccgccgt ggcatgaacg ctaccgccta agtagcgcac atcgtcgatg
    22321 acccaacact ggccttcctg aatcattaac acttcatcct gccagccctg gtcaccctgt
    22381 ttgagatcca cccgcaatgg aatgttacgg gcatcacgat tagggatagt cgatgcactg
    22441 gcaacgtggg cgctgtctgg caaggtggtt cgactggaga acggatcgct gcttaacaat
    22501 tcacgatgac tattatcgcg gctggcatcg ctaagttggg ttgccagttt gtcactcaaa
    22561 taggggcgca gggcggtaat gtcattgctg cgatgttgaa tgcggtagtc gtagaattgt
    22621 tgagccacac tgtcaggccc tccctggaca caggaaccgg tgcgggggcc gttatcttta
    22681 taagctggcg tgacggtggt acaggcactc agcagcagtg cacatggaat aagtagagat
    22741 agtgaacggt agcgcataat catttcctta ttagcttgct gtctgcgtca cactacgacc
    22801 aaacgctatc agattattta atacattgtt tttaatacaa ttctgaaaca atctgaacac
    22861 ctgattgggt ttgagtccat tgctggctac ccactcaagg ttgctcgtag ctgacttact
    22921 taaattaccc aaacaataga cagtgtaaac catttgtgag gttgagcgtg ttatatggct
    22981 gaaatgatca tttaaggtaa atatgatagt tctgaaatgg tggtataaat cctcctgtag
    23041 aggttaagag catctgcaag aagccagttt cacatttata acgcgtgata aatgaatgcg
    23101 gttattgtct aatcattaca aaatttttca catgcgataa gagatcctgc gattaagatg
    23161 attgccaaaa ttacgttgta tttttgaaaa tgagcgaaat ttgatagtcc cagctttgac
    23221 tctcgataat attatttatg ctacaattgt gggtaaagaa tttaagctaa tgaatttgag
    23281 tgaagaataa attaaattgc ttcagtattt cgttaattaa tataaataca aaaggcgttg
    23341 tgcttttata attataaagc gcgaggagtt cggtggatta tcgtttttct tgggtgattt
    23401 ttaatatgat aaacaatata tctgatcaag catcatcatt tccaggcaca caactaaatc
    23461 aaagtgataa tttcctggat tcactgaggg aatttttcgc gatattaaat tccagtcgta
    23521 agggtgagtt gtctacatgg gataccatct acttgcatct gatattagca ataaatgctg
    23581 acagtgatct cataaaaaat gatgtgttgc tcgcggaaaa tattccatca gcgaattacc
    23641 aatttaatac attttttagc aatactttcg agattgatgt taaaaaatat ctgggtaaat
    23701 cagaagataa cgaagttgaa ataaaagcgg gtaatgaaag aatctctatt ggaatacgta
    23761 atgtatcgaa tggaaaactt gaaagacaac agtttctttt cccactggac tatgagaata
    23821 aattacaaga acaattagat aagtatttta cgattgaatc gcacccatta ttatacaggt
    23881 ataccattgg tagtaagatt gcgaatgtga tttttgaaaa gttatacagt cgtattgact
    23941 ttaacaaaga acaatatata tcctttataa aggacgcatt tattcatttt tatgattata
    24001 gcaggcgata tgctattagt gagaatatcg ataaagacgc tgtaacaaac aatatcgcat
    24061 tgatgagcac tttttatgac agtgataata cgtcaggtga ggttttaaat aatgatttta
    24121 cagaagaaga gtcatttgaa actgcgttag atgtagagca tgcaatagtg cttggatttg
    24181 ctgatgataa ttttgaaaca aaacctgttc attatcagga tttattaaca cgatttagtg
    24241 cctttcaaga taccgtgttt aatctgtttc ctgaaatgca ctcttcgcat taccacgata
    24301 tttgtagcgt aagtgtggat atgacaaagg gaacgcaatg catgattcac ttgatggtga
    24361 atgaagaagt tttcatgtct ctgcctgttc cagttgccac gatggtacgt gaagatgcaa
    24421 gtaatttagt caatcttaaa acactactca atgatggttg tttcattaaa tatagtcatt
    24481 ttaatgacgt ggcattaata aagcaaaaca taagtaatct ctatttatca cacactgttg
    24541 tgaatgaaag catattgaaa aaatgttgtt ttgaaaacgg cagtttaggt gatgttaaaa
    24601 taacaaatag caatgtaata aacagtgcat tcaaaaatat ttcgtttaga tctgttaaaa
    24661 ttaataacgt aaatactcat tctttaaaat ttattaactg caattttttc aatgttgata
    24721 tgataagagt taatctcagc aaatgtcttt ttcatgaatg tagtatgcat ggtgtcaaga
    24781 taaaaccctg gttgcctgta aaatggacaa aagaattaat aagtgattat ttgtatggct
    24841 gtttactatc tctgtactct atctgtgcaa gggatatata taatatgaac gccgggaata
    24901 acgtgaaggt tgctgcggat gctttccttg aaattatttt ctctcttaaa aataaatatt
    24961 gtattaaact attaagcgct caggatcggg cgtttatata cgagtttgca agaatgatct
    25021 tcgcttatat taatgacaaa agcattgaga tcttattact ttcttgtttt gcggcggctg
    25081 accaaaaagc aatccagcgg tatagacctc agtcacaaga tggggaagac tttaggagtc
    25141 atttacaata taagttgcct ctttccgctc attaatgttg ttggtgcaac ataataagac
    25201 aggtaagcgg taccctataa tgaaaccaat atcgggttat gcaacggata attgtaaaga
    25261 cttaagtggg tgtttttgac acaatgagat gatttcctaa actgatcaaa accttcttcc
    25321 caacatggca tatcagtccg ataatgcttt agagatcgaa ggcttaacaa acaaggagat
    25381 cgatcatgca attttctaca accccaactc tggaaggcct gaccatcgtt gaatattgcg
    25441 gtgtggtgac cggcgaagcg attttaggtg ccaatatttt ccgtgatttc tttgccggta
    25501 tccgcgatat cgttggcgga cgttccggtg cgtatgaaaa agaactgcgt aaagcacggg
    25561 agatcgcctt tgaggaatta ggctcccagg cgcgggcgct gggggccgat gccgtagttg
    25621 gtattgatat cgactacgaa acggtcgggc aaaacggcag tatgctgatg gttagcgtca
    25681 gcggtacggc ggtgaaaacg cgtcgatgag aagagtcttc tggctggtcg ctgccgctct
    25741 gttactggca gggtgtgcag gcgaaaaagg cattgtcgag aaagagggat atcagcttga
    25801 tacccgacac caggcgcagg cggcgtatcc gcgcattaaa gtgctggtga tccactacac
    25861 cgcagatgat tttgatagct cgctggcgac actgactgat aagcaggtca gctcgcatta
    25921 tctgatccct gcggtgccac cgcgatacaa cggtaaaccg cgcatctggc aactggtgcc
    25981 ggaacaagaa ctggcctggc atgcggggat tagcgcctgg cgcggggcaa cgcgccttaa
    26041 cgacacctct attggcattg agctggaaaa ccgtggctgg caaaaatcat ccggagtgaa
    26101 atattttgcc ccgtttgaac aggcacagat tcaggcgctt attccgctgg cgaaagatat
    26161 tattgcccgt tatcacatca agccggaaaa cgtagtggca catgcggata tcgcaccgca
    26221 gcgcaaagac gatccggggc cattatttcc ctggcagcaa ctggcgcagc aggggattgg
    26281 tgcctggccg gatgcgcagc gggtgaactt ttaccttgcc gggcgcgcgc cgcacacatc
    26341 ggtagatacc gcgtcattgc tggagctttt ggcgcgctac ggttatgacg taaaacctga
    26401 tatgacaccg cgcgagcagc ggcgcgtgat tatggcattc cagatgcatt tccgcccgac
    26461 gttatataac ggcgaagcgg atgcagaaac tcaggcgatt gccgaagcat tgctggagaa
    26521 atacgggcag gattagcgcg gcagttttcc gtggtcgcgt agccaggcgg cagttttctc
    26581 gatgccttca tccagggtga tgaccggctg ataacctaac tcttcctgcg cacgcgtaat
    26641 atccagcgta aagtcaaaat tcaacttgga gacgccgtag tgggtcagcg gcggctcttt
    26701 tgctgacttg cggcctaaac gctccatgct gcgggcgatc atatccagca tcgggtaagg
    26761 gacggaacga atacgacagt caatattcaa ctcgtcgatc agcttctgca cgatgctgcg
    26821 cagtgtgcga tgctcgccgt tggtgatgtt gtacacacgc ccggaaggta gcttatcgca
    26881 ggcttcctgg cttgccagcc acattgcgtg cacggcattt tcatagtagg tcatatcgac
    26941 cagcgcactg ccgccatgcg gtaacagaat actgccgtag tggtgcatca tatgcgccag
    27001 acggggaata aagactttat cgtgcggtcc gaacagactt tgtgggcgca gaatagtaaa
    27061 gcgcgtttgt ggattcgcct gcgaaagcat attgatcact tcttcgctgg ctgctttgct
    27121 gcgggcaaac tcgttggcga agcggtgagg gcgaaaatct tctttaatat tgcgatggtg
    27181 gtgataatcg aagtacaggg agggggaaga gatatgaata aagttacgca caccccaggc
    27241 gacagcccat tcacccaggc ggcgagtggc gcgaacgtta gccagatcga aagcctgttg
    27301 tgtcccccag ggtgaggtaa agctggagca gtgccacagc gtatcaatgc ccgcgagcat
    27361 cactttagct tgtgatgaaa ccagctcggt cagatccgtc ggaacaaact ctgcgcccat
    27421 tttttccagc aatttaccca ttgcttcgtt gcgaccgctc gcacgcacgc tgatgccttt
    27481 ctggcataaa aactctaccg cgtttcgacc taagccgctg gtggcgccgg taaccagtac
    27541 cttcatatca atccactgtt gttgagaaaa tgacgtgcgc attcttccgt gatttccccc
    27601 atgatgcaat gggaaacatg aaagaataac gcaggttttg tcgattaatc tgtgctttgt
    27661 tctgccagtc tggcgatttg ttttgccatt ccgcgaaaaa taaacagatg cgcggggatc
    27721 atcaataacc agtaaaacag ccccggcata ccgtgcggat gccagaaagc gcggacatcg
    27781 atagtacgat agtcaccttt atcttccagg gtaaaacaca gtcgtcccag ccccggcgct
    27841 ttcatgccaa ataacaacgt aagttgcttt ttcggttcaa cgacaatcac tttccagcta
    27901 tccaccgcat cgccggtctg taaatattcg cgctccgggc ggcctttcgc cagattatga
    27961 ccgatcgcgc ggtccatcaa cgcccgtgtc tgccacaaaa tattgccaaa gaaataacgc
    28021 tctttaccgc cgatttggtt caccaccagc cataaagccg caaggctggc ggacgtttta
    28081 acggtaaacc ccgcctgttt ggcgaaataa ccgtactccg gtcgccagcg ggcaaaggcc
    28141 tgagcgtcgt agccccagtc gctggagttg accagttttt cttcctcttt caacgtgcta
    28201 cgtaccgcgt catcgaaagc gatcagccgt tgtgggatga gtgcacgtag cgcggtatca
    28261 tctgccagca gatcgtgttt cagcccctga atcaacgccc tggcggtggt gggcggtacg
    28321 gaagtaatca cattgagaaa ccacaccgaa atccagcggg tggggagggg gatggggatc
    28381 aaccagcggc gcttaccgct caccgccata aaatgttcaa actgttgctg ataactgagc
    28441 acctctggtc cggcggcttc gaagatgcgg tgttcgttgg ctggatggtc taacagcgcc
    28501 accagatagt gcagcaagtt ttccagcgcg atgggcgtgg tgcgtgaacg tacccagcgt
    28561 ggcggcgtta acactggcag gttgtagacc atatcgcgca tgacttcgaa cgccgctgaa
    28621 cctgcgccaa cgataattcc ggcccgaagt tcggtcactg gtacattcgc ttcacgaaga
    28681 atgtccgccg tagcctgacg agctcgcaga tgatccgact gctcatgtgg cggggcctgc
    28741 aacgaactga gaaagattaa ttgcttaact ggtacttcac gtagcgcatc gcggacgttg
    28801 agagccagct ggcgctcctg agcgataaaa tcgccgcctt cgcccatgct gtgcaccaga
    28861 aaatagacgg tatcgatatc ctgcaacagg gccggaaggt tatccggcca gctgagatcg
    28921 actttatggc aactgacgtt tgccagttgc agctttgcaa gcctgtcgac atgacgtgcc
    28981 gccgctagga tctgatgccc ttgctggctg agtgtgcgca ccagatgctg accaatgtag
    29041 ccactggcac cgagaactaa aatgcgttgc ggcacgtctc tctccttaac gcgccaggaa
    29101 tgcgcgccag tgggcggcga cttccgccag ttgttcgcgc gagacgtcaa gatgcgtcac
    29161 caggcggaca atcggcgagg cgttaatcag cacgtttctc gctttcatgt attcgcctaa
    29221 cgcggcagca ttttcttccc cgacgcgaac aaacagcata ttagtgtcct gacgcatcac
    29281 atccgcgcct gcttcacgca gttgctccgc catccaggcg gcgttgtcgt gatcttcctg
    29341 caaccgtgcg acgttatttt tcagggcata catcccggcg gcagccagaa tcccggactg
    29401 acgcatcccg ccaccggcca ttttccgcca gcggatggca cgtttaatgt aatcacgatt
    29461 accgacgagt aatgaaccga ctggcgtccc aagacctttc gacaggcaaa tggtgaacga
    29521 atcacaatat tgcgtgatct ctttcagttc gcagccgtaa gccaccacgg cattaaagat
    29581 gcgcgcaccg tcaacatgca gcgccagatt gcgctcgcgg gtaaattccc atgcttcttt
    29641 caggtattca cgcggcagca ctttgccgtt gtgggtgttt tccagactga gtaatttggt
    29701 gcgggcgaaa tggatatcgt cgggtttgat tttcatcgcc actttatcca gcggtagcgt
    29761 gccgtcggca gccgcgtcga tgggttgcgg ctgaatactg cccagcaccg ctgcgccacc
    29821 ggcttcaaac agatagttat gcgcggcctg accgacaata tactcttcgc cacgttcgca
    29881 gtgactgagc agagcgacca ggttggcctg agtgccggta ggcagaaaaa tggcggcttc
    29941 tttaccggaa agctctgctg catagtcctg cagagcatta acggtagggt cgtctccgta
    30001 aacgtcgtcc ccaaccgggg cggccatcat cgcttcgagc atggcgcggc tcggtcgggt
    30061 aacggtatca ctgcgtaaat caatcatggc atgtccttat tatgacggga aatgccaccc
    30121 tttttacctt agccagttcg ttttcgccag ttcgatcact tcatcgccgc gtccgctgat
    30181 aattgcgcgc aacatataca ggctaaaacc tttggcctgt tcgagtttga tctgcggtgg
    30241 aatggctaat tcttctttgg cgaccaccac atccaccaac accggaccgt cgatagagaa
    30301 ggcgcgttgc agggcttcat caacttcaga ggctttttct acacggatac ccgtaatgcc
    30361 gcacgcttcg gcaatgcggg caaagttggt gtcgtgcagc tcagtaccgt ctgtcaggta
    30421 acctccggcc ttcatctcca tcgccacaaa gcccagcacg ctgttattaa agacgataat
    30481 tttcaccggc agtttcatct gcactactga gaggaaatcg cccatcaaca tgctaaaacc
    30541 gccatcgccg cacatggcga ccacctgacg ttccggctcg gttgcctgcg cacccaacgc
    30601 ctgcggcatg gcgttagcca tcgaaccgtg gttaaacgaa cctaacaggc gacgcttgcc
    30661 gttcattttc agataacgtg ccgcccatac cgttggcgtg ccgacgtcac aggtaaagat
    30721 ggcgtcatcg gcggcaaaat gactaatttg ctgcgccaga tattgcgggt gaatggcttt
    30781 ctcgctcggt ttagctaaat cgtccagccc tttgcgggca tcgcggtaat cttccagcgc
    30841 tttatccaga aacttgcgat cggttttttc ttccaccagt ggcagcaatg cgcgcagggt
    30901 tgatttgata tcgccgacca gcgccatatc taccttgcta tgcgcgccga tgctggctgg
    30961 attaatatca atctgaataa ttttggcatc ggtcgggtag aacgcacggt aggggaactg
    31021 agtaccgagt agcactaacg tgtcggcgtt catcatggta tggaaacctg acgagaatcc
    31081 gattaacccg gtcattccaa catcatacgg attatcgtat tcgacatgct ctttaccgcg
    31141 tagggcatga acaataggtg ctttaatttt cccggcaaac tcaactaact ctttatgcgc
    31201 ccccgcgcag ccgctgccac acatcagggc gatattgctg gaataacgca gcagttgcgc
    31261 cagtttgcgt aactcttctt cttccggcat cacgactggc tgtggcgcat gataccagtg
    31321 cgtggttgcc ccttctggcg caggttttaa cgccacgtcg cccggtaata caacaacgga
    31381 aacgccacgg ttaagcaccg ctttgcgcat agcaattgcc agcacttgtg ggatctgctc
    31441 cgggctggaa accagctcgc aatagtgact acattcgcgg aatagctctt gtgggtgggt
    31501 ttcctggaaa tagccgctgc caatttcgct ggagggaata tgagcggcaa tcgccagtac
    31561 cggaacgtta ttgcggtggc aatcgaatag gccgttgatc aagtgcaggt tgcctgggcc
    31621 gcacgatccg gcacagaccg ccagttctcc tgaaagttgt gcttcagcgc cagcggcaaa
    31681 cgccgccact tcttcatggc gggtggacat ccactcgatg gtgcccatgc gattaagact
    31741 gtcgctaaga ccgttcagag agtcgcccgt gactccccag atgcgtttca cgcctgccga
    31801 ttcgagtgtt ttggcgatat aagctgcaac cgtttgtttc atggttctcc atctcctgaa
    31861 tgtgataacg gtaacaagtt tagttcatct gacggagggg gaagggatgg gagagaaagg
    31921 aggcactaac ggttaaatag cccgatgaaa ggaatatcat cgggcataag gcgattatgc
    31981 gagaaccaaa tccccctgcg gatggcagga gcaggccagt acgtaacctt cagcgatttc
    32041 ggcgtcggtc agcgtcattg tactgctcac cgtatattca ccggaaacca cttttgtctt
    32101 acagcagccg caaacacccg cacggcaggc agcgacaacc ggaacgttat tgctttccag
    32161 cgcctccagt agcgtggtgc caaccggggc gtaaaattct cgtgccggtt gcagtttggt
    32221 gaatttcaga ccgctggtcg ccgcttctgc tactggggtg aagaatttct ctttaaagaa
    32281 acgcgtcacg ccgagcgctt tcacttcctg ctctacccaa tccatatacg gagccgggcc
    32341 gcaggtcatc acggtacgtg aagctaagtc aggtacatct gccagcagtt cgcgagtgag
    32401 acgaccagcg ataaagcctt cggtaacgtt attttctgcc accagcgtta ccggatagtt
    32461 acgccactca tcggcgaaaa taacatcctg cggcgtacgc acgttgtaga tcacccgcac
    32521 atcggcctgt ggacggttct tcgcaagcca gcgacgcatc gacataatcg gcgtgacgcc
    32581 gcagcctgcc gccagcaaca ggaatttatc ttctgcttta tcgtcgcagg taaattcccc
    32641 catcgcgtcc gaaagccaga gataatcacc gcgttttaca tcgcgcgtca gccactggga
    32701 gccgacaccg tcatcaatcc gccgcacggt cagggtgata tattcactca cgcctggcgt
    32761 ggaggaaatg gtgtaagcac gcagcgtttc cgctgagttt cgcacgctga ccagtgcata
    32821 ttgcccggcg cgatatgggt agtgatcgtg gcaaatcagg gaaatcgtcc acacatccgg
    32881 cgtttcttgc gtaatgtgat gaacctgtat ccgccacggg cattgattcg ttggcatcgt
    32941 catcgacaaa ctccttacgc gttcaacagt tgcttcatgt cttcttcaac agtggtgata
    33001 gaacgcaggc cgaatttctc gttcagcacc gccagcaggt ctggtgtcag gaaaccaggt
    33061 gcagtcgggc cggtgacgat atttttcacg cccagagaaa gcagcgtcag cagaatgaca
    33121 atcgctttct gttcaaacca ggagagcacc agcgacagcg gcagatcgtt cacaccacag
    33181 cccagtttct ctgccagagt gacagccaga ataattgctg agtaagcatc gttacattga
    33241 cctgcatcta ccagacgcgg cagaccttcg atatcgccaa actcaagttt gttaaagcga
    33301 tatttaccac aggcgagggt caggatcagg cagtcatccg gcacgctggt ggcgaaatcg
    33361 gtgaagtagt ggcgctcgcc gcgtgcgccg tcacagccac caagcaggaa gatatgacgc
    33421 agtttttcac ggctaaccag atcaatcagc gtatcggctg caccaagcag cgtctggcga
    33481 ccaaagccca cggtgatcag gtgaggaatt tcgctgtacg ggaagcccgc catttgttgc
    33541 gcctgggcga tgaccgcaga gaaatcttcg ccatccagat gacgcacgcc aggccagcca
    33601 acgatgctgc gggtccagat acgatcgtca taagcgccta cggttgggtc gatgatgcag
    33661 ttcgaggtca tcacgatggg gccagggaaa cgagcgaact ccacttgctg attctgccag
    33721 ccgctgccgt agttaccgac cagatgcttg aatttacgca gctccgggta gccatgcgca
    33781 ggcagcattt cgccgtgggt gtagacatta acgcccgtgc cttcggtctg ttccagcagg
    33841 ttgtagagat ctttgagatc gtgaccggaa atcagaatgc atttacccgc cgtcgctttg
    33901 acgttgacct gggttggcgt cgggtgaccg tatttaccgg tttcgcctgc atccagaatg
    33961 ctcatcactt tgaagttcat ctggccgatt tccattgaac actcaagaag cgcgttcata
    34021 tcggcaggcc aggtccccag ccacgccatg attttatggt actgggcata aatatcgttg
    34081 tcgtattgac cgagaacatg cgcgtgttcc atataggccg ccgcaccttt caggccatac
    34141 aggcacagca gacgcaggcc gagaatgttt tcgccaatcg ccgctttatc tttgttaggg
    34201 gtaaattctg ctgcctgacg ttgcagctcg ccgagatcat cgctcaccag ttgcaggtca
    34261 gccatcgggt tatcgacgcg cgcgttggca tctacagcca ggcattgtgc tttcagcgcc
    34321 tcgcgcaagg caatcgcttc acgagcgtag ccgacaatac gcggagaatc gaagttaacg
    34381 ttggtcaggg ttgagaaaaa ggcacgtggc gcgaagctgt caacatcgtg gttgatgatg
    34441 ccgtattcac gcgcttttac cgcccaggca gaaagccctt gcagcgccgc gatgagtaaa
    34501 tcctgaaggt cagaagtttc cgccgtttta ccacacatcc cctgcgcgta tgagcagccg
    34561 ttttctgccg gagtacggat agtttgttca cattgcacac aaaacatgat cacacctttt
    34621 aaagttatat ttaatataca tgtttaaggg taagacgctt aacgcgggga taaaagggat
    34681 ttttcatgca actttaaggg agattgattt agcgcaattt tggcggcagg gaactaccgc
    34741 cagagaggta ttacgcagag aaaaaggcga tgaggatcgg cactaacagg ctaagaataa
    34801 aaccgtgaac aattgccgcc gggaccatat ccagcccgcc agtacgttga agaacgggca
    34861 gggtgaaatc cattgatgtg gcaccgcata agcccagtgc agtagagcgg ctgcggcgaa
    34921 tcagcccagg gatcaacata atagcaatca gttcacgggc cagatcatta aaaaacgccg
    34981 cgctcccgat taccggacca aaagattcgg tcaataaaat accggaaaga gaataccagc
    35041 cgaaaccgga ggccattgcc agcgcggtat tgatggggag atcaagaata aaggcgttaa
    35101 ttaaaccacc aattaatgaa ctgacaacca ccaccacggc gacaatcatt ccccggcgat
    35161 taaggacaat ctgctttaag gtcatgccat tattgcgcaa ctgaatacca acgaggaaaa
    35221 gtagcaaaat taacgtgtat tcactggctt cggtcgcgtg ttgtaagaaa gccagtccac
    35281 tcagaccaat ggcaaaacca atcactacta cgccgcacag ttttagcgac tccagcgcca
    35341 tcgcaatacg cgacgggagt ttttcttgct gatggtggtt gcgccacggc aggcctcgct
    35401 ccagccacat cagggcggca atattacaca gtaaaataac ggtaatactg acggcagaat
    35461 aatgcagaat cgccaacagg ttactggcga ggttatcgag aaacgccaga ctgataccca
    35521 taaaaaagag aataaggtaa accatccagc ttaatagctg attaatagct tttaacgcag
    35581 cttgttggcg aagcggaatg aggtaaccca caatcagggg aaccagaatg attaacagcc
    35641 cagaaaacat gaaaatccag tccttgcaaa gaagaagtcg aaatgcgcga tgacacacta
    35701 ctgaaagcgg aagggcgagt aaagttgcaa ttaaaaggaa atgttatgca tcagaggggg
    35761 atttttaatc ctatctgagt gtttagcatt gttgcgctcc tttcatgtgc aatgccatat
    35821 aaagccactg gcggagggag cgcgcagggg gcagccagtc gccgccgccc cctgcacccc
    35881 cgggctctgg cgaacaaaat cgccgctgcg cggtaccttc agcttatccc cttcggcaac
    35941 cgggtcgggc gcgaggtaac gtcctgtaag acgcgccctc agcccacatc catgtgggct
    36001 gccccggcct tcggggaacg cttcagcgat tttgacgcca ccaatacccg tgcggtttgt
    36061 aatgtcgaag ataaaacaac cagggcagat aaaatgccaa tgctaattaa tcacgctttt
    36121 ccagcagagt ccggtaaatc agaccaccga taatgccgcc gacaattggc accacccaga
    36181 agaaccacag ttgttctaat gcccagccgc cctggaagat agcaaccgcg gtgctgcgcg
    36241 ccgggttaac agaagtgtta gtcaccggaa tactgattaa gtgaatcagg gttaaggcca
    36301 gaccaatagc gatcggcgca aaacctgccg gagcgaattt gtcggttgca ccgtggatca
    36361 ctaacaggaa gcccgcactc aataccagtt caactaccag cgcggaaagc atggaataac
    36421 cgcctggtga atgctcgcca taaccgttag aagcaaaacc gctggctgcc gcgtcaaaac
    36481 ccgttttacc actggcaatt aaatacagca gcgccgctgc aacaataccg ccgacaacct
    36541 gggcaattac gtagccaacg acttcttttg ccggaaaacg tccgccagcc cataaaccaa
    36601 tagtgaccgc cgggttaaaa tgaccaccag aaatatgacc aacagcgaag gccatcgtca
    36661 gaacggtcaa accaaatgcc agcgccacgc cggcaaaacc aatgcctaat tccgggaagc
    36721 ctgcggccag tacagcacta ccacagccac caaaaacaag ccagaaagta ccaaaacatt
    36781 cagctgctaa ttttctgaac atatccacct caattaaaaa ttgaccctgt gaa
//
LOCUS       DAEEUE010000031        36520 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.031, whole genome shotgun sequence.
ACCESSION   DAEEUE010000031 DAEEUE010000000
VERSION     DAEEUE010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36520
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.031"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(156..1391)
                     /locus_tag="KLK84_001693"
     CDS             complement(156..1391)
                     /locus_tag="KLK84_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HBE2009058.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="KLK84_001694"
     tRNA            complement(1538..1614)
                     /locus_tag="KLK84_001694"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
     gene            complement(1657..1743)
                     /locus_tag="KLK84_001695"
     tRNA            complement(1657..1743)
                     /locus_tag="KLK84_001695"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Leu,seq:cag)
     gene            complement(1764..1839)
                     /locus_tag="KLK84_001696"
     tRNA            complement(1764..1839)
                     /locus_tag="KLK84_001696"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:His,seq:gtg)
     gene            complement(1898..1974)
                     /locus_tag="KLK84_001697"
     tRNA            complement(1898..1974)
                     /locus_tag="KLK84_001697"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:ccg)
     gene            complement(2077..3462)
                     /locus_tag="KLK84_001698"
     CDS             complement(2077..3462)
                     /locus_tag="KLK84_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBE2009059.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="KLK84_001699"
     CDS             complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="KLK84_001699"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HBE2009060.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="KLK84_001700"
     CDS             complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="KLK84_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HBE2009061.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="KLK84_001701"
                     /gene_synonym="wecF"
     CDS             complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="KLK84_001701"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HBE2009062.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNMMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="KLK84_001702"
     CDS             complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="KLK84_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HBE2009063.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="KLK84_001703"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="KLK84_001703"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HBE2009064.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="KLK84_001704"
                     /gene_synonym="wecD"
     CDS             complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="KLK84_001704"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HBE2009065.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="KLK84_001705"
     CDS             complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="KLK84_001705"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBE2009066.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="KLK84_001706"
     CDS             complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="KLK84_001706"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBE2009067.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="KLK84_001707"
     CDS             complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="KLK84_001707"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HBE2009068.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="KLK84_001708"
     CDS             complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="KLK84_001708"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBE2009069.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="KLK84_001709"
     CDS             complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="KLK84_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HBE2009070.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="KLK84_001710"
     CDS             complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="KLK84_001710"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HBE2009071.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16673..17932)
                     /gene="rho"
                     /locus_tag="KLK84_001711"
     CDS             complement(16673..17932)
                     /gene="rho"
                     /locus_tag="KLK84_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBE2009072.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="KLK84_001712"
     CDS             complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="KLK84_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HBE2009073.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="KLK84_001713"
     CDS             complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="KLK84_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HBE2009074.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18719..19984
                     /gene="rhlB"
                     /locus_tag="KLK84_001714"
     CDS             18719..19984
                     /gene="rhlB"
                     /locus_tag="KLK84_001714"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HBE2009075.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20118..21602
                     /gene="gppA"
                     /locus_tag="KLK84_001715"
     CDS             20118..21602
                     /gene="gppA"
                     /locus_tag="KLK84_001715"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HBE2009076.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21649..23670)
                     /gene="rep"
                     /locus_tag="KLK84_001716"
     CDS             complement(21649..23670)
                     /gene="rep"
                     /locus_tag="KLK84_001716"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HBE2009077.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23757..24038
                     /gene="ppiC"
                     /locus_tag="KLK84_001717"
     CDS             23757..24038
                     /gene="ppiC"
                     /locus_tag="KLK84_001717"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HBE2009078.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="KLK84_001718"
     CDS             complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="KLK84_001718"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBE2009079.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25709..26599
                     /gene="ilvY"
                     /locus_tag="KLK84_001719"
     CDS             25709..26599
                     /gene="ilvY"
                     /locus_tag="KLK84_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HBE2009080.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="KLK84_001720"
     CDS             complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="KLK84_001720"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HBE2009081.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="KLK84_001721"
     CDS             complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="KLK84_001721"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBE2009082.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="KLK84_001722"
     CDS             complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="KLK84_001722"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HBE2009083.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGNVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="KLK84_001723"
     CDS             complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="KLK84_001723"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HBE2009084.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31267..32913)
                     /locus_tag="KLK84_001724"
     CDS             complement(31267..32913)
                     /locus_tag="KLK84_001724"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HBE2009085.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32916..32966)
                     /locus_tag="KLK84_001725"
     CDS             complement(32916..32966)
                     /locus_tag="KLK84_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009086.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="KLK84_001726"
     CDS             complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="KLK84_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HBE2009087.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33502..35022
                     /locus_tag="KLK84_001727"
     CDS             33502..35022
                     /locus_tag="KLK84_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBE2009088.1"
                     /translation="MSLSIVHTRAALEVNAPPITVEVHISKGLPGLTMVGLTETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKNTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(35047..35385)
                     /gene="maoP"
                     /locus_tag="KLK84_001728"
     CDS             complement(35047..35385)
                     /gene="maoP"
                     /locus_tag="KLK84_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HBE2009089.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35504..36343
                     /gene="hdfR"
                     /locus_tag="KLK84_001729"
     CDS             35504..36343
                     /gene="hdfR"
                     /locus_tag="KLK84_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HBE2009090.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36439..36514)
                     /locus_tag="KLK84_001730"
     tRNA            complement(36439..36514)
                     /locus_tag="KLK84_001730"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36479..36481),aa:Trp,seq:cca)
BASE COUNT         8918 a         9996 c         9200 g         8406 t
ORIGIN      
        1 gccaccgcac gtaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gctatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     1681 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     1741 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
     1801 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
     1861 gtattactac caccgcagct caagcgccgg tactaaatgg cgcgcccgac aggattcgaa
     1921 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
     1981 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
     2041 attgctcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt
     2101 accgtggcga ttaagcccaa aaactttata aatcgccgtc accgccagca taaagatgat
     2161 gccgacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
     2221 caggaatgcc attgttacgt agttggccca cgggaacagg attgagcgga acggatggct
     2281 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
     2341 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
     2401 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
     2461 cgcaaccggt acgccgtgac gggaaacttt tgccattgcc gccggtaact gacggttttt
     2521 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
     2581 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
     2641 agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat
     2701 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacggcact
     2761 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
     2821 ctggtaggac gccaccacaa tacacagagc ggtcaggaac cctttccagc cacccgcaaa
     2881 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
     2941 aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga ctttgatcat
     3001 cgcgaaccag aactcgattt cgccgtacaa ccgcaccgcc gccagattcg ccaacgccac
     3061 cagcgccacc gcgatcaatg cgggtatcca ctgcgccatc tccggggacc aaaactggac
     3121 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga
     3181 ccaggcggtg agatagccaa aaaacgggct catataacga tgcgcataaa cggcgaacga
     3241 accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga aaaaaacgaa
     3301 cagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc
     3361 ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt cgatatgtcg
     3421 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
     3481 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
     3541 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
     3601 aagcagacag gcgacgaagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
     3661 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
     3721 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
     3781 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
     3841 gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg
     3901 tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt
     3961 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
     4021 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
     4081 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
     4141 gcgggtactt tttacgtact gaacgtacaa cgctgatgcc atccgcatat ttaaattcgg
     4201 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcggcat
     4261 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcat
     4321 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
     4381 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
     4441 aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca gacatgcgcc
     4501 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgtgccag
     4561 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
     4621 attagtctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagtccaac
     4681 cacaatcgcc ccgagtggga tgaacaacgc gccgcccatc accaccagtg agcctataag
     4741 cgtaggcgag atcgccagtc cggagtggtt attcagtact tcccaggtaa agtaattggc
     4801 tgagttcagc accatactcg ggcgccccgg ccacagccag gaagggataa agacatagaa
     4861 atcgcggaca attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
     4921 cgccagattc tcccacggtg aaaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
     4981 ttcatcgccg ctcacattca ttccatagcg ttttaacgcc agccagaaca tgccaacaat
     5041 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
     5101 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
     5161 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg
     5221 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
     5281 cacgccggag acttcactgg aaaagatctg actgtaggag ttaagccgga acagcaaaaa
     5341 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca
     5401 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
     5461 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
     5521 aagcaacacc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
     5581 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
     5641 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
     5701 gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg
     5761 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
     5821 gtgtttttat ccgccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
     5881 tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca gaacggattt
     5941 tccctgttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
     6001 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
     6061 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccagcccc
     6121 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
     6181 acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac atgctcattg
     6241 ctgcggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg ccgatcgttc
     6301 gccatcatat tgagagaagg gtccatccgc gtcgggaagt acagcagttc tccccgtacc
     6361 tttgggtgcg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac acagccgact
     6421 cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc actggaaagc
     6481 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
     6541 agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg
     6601 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
     6661 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
     6721 cgcgcgtgct cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta
     6781 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
     6841 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
     6901 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
     6961 actggctgac ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac
     7021 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
     7081 gcatagcggt aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga
     7141 cggtaaaact cgctgccgcc actgccggta agacgaattt cagcgattta accacttccc
     7201 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
     7261 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
     7321 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
     7381 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
     7441 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttaatc aacatgatgg
     7501 cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
     7561 cgcccaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
     7621 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
     7681 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
     7741 caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
     7801 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
     7861 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagccccg gcaagcacgc
     7921 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
     7981 acaccgccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
     8041 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
     8101 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
     8161 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagtg ttcccccgca
     8221 gggcaaccgt gcagcggaat gtaatgaaac accgccatga tttccgcttc tttcagaaag
     8281 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
     8341 tgcacgcagc catcgggaat cgacggcagc tcgatacgcc cggctttcgc cagaggcgct
     8401 aacgtatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
     8461 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
     8521 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
     8581 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
     8641 tcaccacccg ccgtgtagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
     8701 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttccaccaca
     8761 aacaaattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
     8821 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ctgcttcaat caacgtttcg
     8881 tcgatgttca tggtgtccgg gcgaacatcc acaaagacga ttttcgcgcc acgcagcaca
     8941 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
     9001 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
     9061 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
     9121 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
     9181 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
     9241 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
     9301 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
     9361 acctgcaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
     9421 agagacatag ccgcgaatat cgccggacgc cgcacgtaga atcagacatt gatgatcaaa
     9481 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgaccgctgg cgtcaggcgc
     9541 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctagctaact gacgtaatgc
     9601 gggaatatcg gtctcttgtg ccacgacagc accgctatca ctgacattgt tcacgggtag
     9661 cgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
     9721 ccccgtattt gatgccgcaa ttttggcctg cacccgtgac catggcgcta aggcgtctga
     9781 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
     9841 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
     9901 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
     9961 gccagccgtt acgccaggcg atctcttcca ggcaggcaat cttaaagccc tggcgttttt
    10021 ctactgtctg cacaaaagtg ctggcctcaa tcaggctgtc gtgagtgccg gtatccagcc
    10081 aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg tacatctggt
    10141 tgatggaggt aatctccagt tcaccgcgct ccgacggctt cacctgcttt gcgtactcca
    10201 cgactttact gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc ggctgttttg
    10261 gcttttcttc aagcgagata gcgcggaaat tgtcgtcaaa ttccaccacg ccaaagcgtt
    10321 ccgggtccat cacctgatag ccaaaaaccg tcgccccttc cgtgcgcgcc gcaacatgac
    10381 gcagcttcgg actgaagccc tgaccgaaga agatgttatc gcccagcacc agacaagaag
    10441 gttcaccatt aaggaaggtt tcaccgatga taaaggcctg cgccagaccg tccgggctgg
    10501 gctgttcggc atattccagc tggataccga actcactacc atcgcccagc aggcgctgga
    10561 aataaccttt atcttccggc gtagtgatga tgagaatttc gcggataccg gccagcatca
    10621 gcaccgacag cggatagtaa atcattggct tatcgtaaat cggcaacagt tgcttcgata
    10681 cgccgcgcgt aatcggatgc aatcgggtac cggaaccgcc cgccaggata atacccttca
    10741 tgtttgcctc cgctgaaaat tagcctttca gacctaaacg ctcaccctga tagctgccgt
    10801 cctgcacctg cttccaccag ctttcattag ccagatacca ctgaaccgtt ttacgcattc
    10861 cactttcaaa ggtttcctgc ggcagccagc caagttcacg ggcaattttc gaagcatcaa
    10921 tggcataacg cagatcatgc cccggacggt cagcgacaaa ggtgatcagg tcacgatagt
    10981 gcgccacgcc gtgcggcttg ttcgaagcca gttcttccag cagctcgcaa atggtttcca
    11041 caacatcgag attcttacgc tcgttgtgac caccaatatt ataggtttca ccgactttcc
    11101 cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc cacatacagc cagtcacgga
    11161 tttgctgccc gttgccatat accggcagcg gtttacccgc cagcgcgttg aggatcatca
    11221 gcgggatcag tttttccgga aagtggtaag gaccgtagtt attcgagcag ttggtgatca
    11281 gcgtcggcag accgtaggtc cgcagccagg cgcgcaccag atggtcgctg ctggcttttg
    11341 acgcggaata agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa tcatccgtcg
    11401 agtgcaggtc accatatact tcgtcggtgg agatatgatg aaaacggaac gctgattttt
    11461 tatcttccgt cagcgtattc cagtaagccc gcgccgcttc aagcaatgta taagtcccga
    11521 caatgttggt ttcaataaac gctgccgggc cgtcaataga acggtcaaca tggctttcgg
    11581 ctgccagatg catgacacag tctggctgat gctcagtgaa tacgcgtgcc agttctgccc
    11641 gatcgcagat atcaactttc tcaaaggcaa agcgctcgct ttgcgcgacc ggtgccagcg
    11701 acatcaggtt tccggcgtag gtcagcttat cgaccactac caccgcatcg ctcgtttcgt
    11761 tgatgatata acgcaccagc gccgagccaa taaacccggc accacctgtt atcagaattt
    11821 ttctcatcag cgccagactc ctttggcatc gacgacatac tgctgatgga cattgtcgcc
    11881 attgataact ttgaactgac tatgatcgac cagcatcacc agcacgtctg ccgttgccag
    11941 cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca actggtggat
    12001 gttaggctca acaaccagcg tttcgccgct gtgccactgg gcgatcagtt cagcgatttc
    12061 catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac caaagcaggc
    12121 gattttcagt tcactggcgc gtttatcggt agcagcaagg cagtcggcaa ccgccgcttt
    12181 cacctgatcg ataacccaga acggtttgtg atcgttaact tcgcgcgcgg tacggataag
    12241 ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcaa tgcagtgacc
    12301 gcccacgcca gggccaggct gaagaatatt aacgcgaggg tgacgattcg ccaggcgaat
    12361 cagttcccag acgttaatcc cctgatcggc acaaatcagc gacaattcat tagcaaaagc
    12421 gatattcaca tcgcggaagc tgttttcggt gagcttacac atttccgccg tccgcgagtt
    12481 agtgacgaca cactcacctt cgaggaaaat tttgtacaat tcgctggcgc gggccgaaca
    12541 aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca taacctggcc
    12601 cggtaataca cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc ccacctgctg
    12661 tgggaaagtc agatccgggc gcatctctgc taaccattct gccatcttct cggttgaccc
    12721 caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg gcgcaatgga
    12781 gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa acggcgtagg
    12841 tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta aaaaaccgcc
    12901 ttctacggca gtttttacta cgctcgccaa atcaggctcg acgatatgga tttcgccacg
    12961 attgatggta tcaaccgcgt gttggttgat atcgacacca atcacctgtt tttgccgtga
    13021 ggcaaacgct gctgctgtag gcagcccgat gtaacccagt ccgataacag aaatggtcgc
    13081 aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga gcatgcctga
    13141 ccatcaccat acgggttatg ggcgcggctc atagcttgat attcgttttc gtcttttaaa
    13201 agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg caccgtaccc
    13261 gccgtcaccg cttccggacg ctcagtggta tcgcgcatca ccagcaccgg tttccccagc
    13321 gaaggcgctt cttcctgaat gccgcctgag tcagtcaaaa tcagccaggc gtggttcatc
    13381 agccagacaa acggtaaata ctcctgggga tcgatcagaa tgacattttt cacatgcccc
    13441 agaatgcgat tgaccggctc tctgacgttc gggttgagat gcaccggata gacaatctgg
    13501 atgtcctggt gcgtggtggc gatgtctgcc agcgcgtggc agatttcttc aaagccacga
    13561 ccgaaactct cacgcctatg accggtcacc agaatcatct ttttatcggg ggcgataaac
    13621 gggtaatttg ccgccagttc tgaacgcagc gtgtcgctgc tcatcacctg gtcacgcacc
    13681 cataacagtg catcaatgac tgtattaccg gtaatgaaga ttcggctatc cgcaacgttt
    13741 tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat cgccagatgc
    13801 ccggtcaatg tacggttagc ctcttccggc cacggcgaat agagagcgcc cgtgcgcaga
    13861 ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct ggttgccagc
    13921 gtcgtcgtcg tatcgccgtg aaccagcacg acgtctggtt tgaactcggc aagaataggt
    13981 tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg ctgcattatg
    14041 ttgagatcgt agtcaggtac aatggaaaag agtttcagta cctgatcaag catctcccga
    14101 tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa cgcatgcacc
    14161 aacggagcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac tttcacatcg
    14221 attctcttcg aataagcggc gagcgccttt gcgctcaccg cagcagtgtt gctatttcga
    14281 gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccgacaatgc cccacataat
    14341 catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg tacgcaaata
    14401 gcgataggtc tgaaaacgcg gatccagggt tggaccaaca ttcagggtgt ttaacatggc
    14461 tcgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat tttccagtcg
    14521 agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt cctcggcagg
    14581 cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg cctgctcaat
    14641 actgttcatc cggcggtcgt agatggcttt cgccacctct tcctgacgct tcacctgagc
    14701 tttcatctgg atggtgcgtg ccgcccatgc gcctttcagc tcatcattca gatggctggc
    14761 tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt caggcgcggt
    14821 ttcggcaata agcttcacgc tgtcattgac cgcgcgagta aagtctccgg ggataaactg
    14881 gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc ccaccatccg
    14941 ctgtttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg aggccagttg
    15001 cataacaaac tctttatagg cttcgtccat gaccgatggt tgatcggcag aagccatgtt
    15061 tgaacggaca tccaggttac gcaaaaattg ctgctgcgag taatatcccc ccagcatatt
    15121 caccgttgga cgatcggtaa tcgccgtcga gctccactcc tgacgagcaa aaaaagtata
    15181 cgccagcgcg attaacgcaa acgccagccc catgccaata atccatagct tcccagccca
    15241 caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg ccggtttccc
    15301 aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct gccaccacga
    15361 tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca ggcacgctta
    15421 atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac ccactccggg
    15481 acaaaatgag aatattctgc cagcacgcca atggaagcga gcagtgctgc ggcaagggta
    15541 atcagcacaa acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg gtgaatatgc
    15601 tgacggtcag gagagaatgg gctcatgcct ttacgcaggc gacggtacat aatcgccacc
    15661 atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat gggatgggtt
    15721 ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag cgtactgccc
    15781 gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag catgatgtat
    15841 ggcaggatgg cggcgatcat cgcaaagcac cagattgcga ggctggtttg cccgtcgaac
    15901 cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag caagccatca
    15961 atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag cgtcaggaag
    16021 taaccaaacg gtccgagcac catctcccag gagccaaaga tataacccag gctactgaga
    16081 taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt ggcacggatt
    16141 tttacgctga tatcaaaacg atcatccagc gcgccaatga aaacaagcac accggcacaa
    16201 gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt gaagcaaatc
    16261 cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg tttgcggaag
    16321 tttggtttat ccactaaacc gacttttttt gccaccttac gggcaaaaaa cagaaacagt
    16381 gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt cacaatgcat
    16441 gctctcagag aaaattatta gcagaagtat aaccacgaag acctttattc agaagggaaa
    16501 gtccgttccg aacccaccag ccctgtgcaa aaatcaacca tttgatatgc aaattgcata
    16561 acatgccagc aaattccagg aattttccta acatcgccag cgcgacatta agattacaga
    16621 tataaaagca aaacgccacg taaacacgtg gcgtttttgg cataagacaa atttatgagc
    16681 gtttcatcat ttcgaagaaa tcgtcattgg tcttggtcat ggccagttta ttaatgagga
    16741 attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc cacattttct
    16801 gcagttcttc ctgagtcgtg agcagctctt ctttacgggt accggaacgg ttgtagtcga
    16861 tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc atgttgcctg
    16921 tacctttaaa ctcttcgtaa ataacttcgt ccattttaga accggtatcg ataagcgccg
    16981 tcgcgataat ggtcaggctg ccgccctctt ccacgttacg cgccgcacca aagaagcgtt
    17041 tcggacgatg aagggcattg gcgtccacac caccggtcaa cactttacct gacgccggaa
    17101 caacagtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg ataacgtctt
    17161 tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc tgaacgtggc
    17221 gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga cgctgcatct
    17281 cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa tccgggtggt
    17341 tgtaagcaat gctctgagca atgttctgca gcagcatagt tttaccggct ttcggcggtg
    17401 ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt acgcgagcag
    17461 ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag acgagagttt gcgtgcagcg
    17521 gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg aagttaactt
    17581 cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga atcttaccag
    17641 agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa acgtagatgt
    17701 catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat ccatcctgca
    17761 atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc tgcttcagga
    17821 tggcaaaaat aatgtcctgc ttacgcatgc gagccaggtt ttccagcccc atattttcgc
    17881 cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc atagtggtgt
    17941 gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg gcaggatcat
    18001 ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca ggagagtacg
    18061 cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca cttcgcattt
    18121 aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt atgtttaata
    18181 cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag cgacggggca
    18241 cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca actgaccttt
    18301 agacagtgcg cccactttgg ttgccgccac ttcaccgttt ttgaacagca gcagagtcgg
    18361 gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt tcagttttgc
    18421 aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg cgatcatttt
    18481 gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg ctttgagtac
    18541 atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat ataactccac
    18601 aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac taaaggttga
    18661 ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct accacactat
    18721 gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc cgaaggttgt
    18781 agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac tggcccttcc
    18841 gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg ggaaaacgat
    18901 ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg ccgatcgcaa
    18961 ggtgaatcaa ccgcgtgcct taattatggc accgacgcgt gaacttgccg tgcagatcca
    19021 cgccgacgcg gaaccgctgg cggaagctac cggcctgaag ctgggcctgg cttacggtgg
    19081 tgatggctat gacaaacagc tgaaagtgct ggaaagcggc gttgacattc tgattggcac
    19141 cacgggtcgt ttaattgact acgccaagca gaaccacatt aacctcggtg ccattcaggt
    19201 agtggtactg gacgaagccg atcgcatgta cgatctaggc tttattaaag atatccgctg
    19261 gctgttccgc cgtatgccgc ctgcaaatca gcgcctcaac atgctgttct ccgccacgct
    19321 ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat atattgaagt
    19381 ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc cttctaacga
    19441 agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca gagcgattat
    19501 tttcgccaat accaaacacc gttgtgaaga gatctggggc cacctggcgg cagatggtca
    19561 tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta ttcttgatga
    19621 atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc gtggtttgca
    19681 tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg aagattacgt
    19741 tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca gcctggcgtg
    19801 tgaagagtat gcattgaatt tgcctgctat tgagacctat attggtcact caattccggt
    19861 aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc gcctcacgcg
    19921 cccgcgcaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc gtcgttcagg
    19981 ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa gagaatgaat
    20041 cccgatgagc ttactgtagt aagtgattcg ggtgaaagaa tgcagccaac acagagacag
    20101 attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt gatctcggtt
    20161 cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc
    20221 gaattaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgac ctctctaatg
    20281 aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc
    20341 ctccctcgca aattcgcgtt gtcgctacgg cgacattacg acttgccgtc aatgcgggtg
    20401 attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag
    20461 aggaagcgcg tctgatttat cagggcgttg ctcacaccac tggtggtgcc gatcagcgcc
    20521 tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca
    20581 cctcgttgtt cagcctgtcg atgggctgcg tcacctggct ggaacgctat tttgccgatc
    20641 gtaatctggg gcaagaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc
    20701 cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct tccggcaccg
    20761 tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa
    20821 agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg
    20881 acgggctgac gctggaacgt gcattagtgt tcccgagtgg tctggcgatc ctgatcgcca
    20941 tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc
    21001 tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt acactgcgta
    21061 atattcaacg ccgctttatg atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg
    21121 ctaacttctt cgatcaggtg gaaaacgaat ggcatcttga agcaataagc cgcgatttgc
    21181 tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgacttcaaa caagctccgc
    21241 aacacgctgc ttatctggtg cgtaacctgg atcttcccgg ttttaccccc gcacagaaga
    21301 aactgctggc gacgctactg ctcaaccaga ctaacccggt cgatctctca tcgctgcatc
    21361 agcaaaatgc cgtaccaccg cgcgtcgcag aacaactctg ccgtttactc cgcctggcta
    21421 tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc
    21481 atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag
    21541 agattattgc tcaggaaaac cagtggcaga gctatgtcca ctggccgctg gaagtgcatt
    21601 aatgagtaag tgccggatgc gatgctgacg catcttctcc ggccttgatt atttccctcg
    21661 ttttgccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc
    21721 ttcggcactg accactttgc gctcctgttc ccaaatcaga tcatcctgcg gcagctccag
    21781 caaaaagcgg ctcggctccg ggcgcaccag ttcgccgtac tgacggcgtt ctttacacag
    21841 cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc
    21901 atcgatatta tcttcatcga tactgctctg gtgcggcaaa aacccttctt ccataccgac
    21961 catgtagaca taaggaaact ccagcccttt tgacgcgtgg agagtcatca gttgcacctg
    22021 atccagctct tcttcactct caccacgctc catcatgtcg cgtaaagtaa agcgcgtcac
    22081 cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct ccgtcatcca
    22141 gctaaacagt tggttgacgt tcttcatgcg catttcggca gctttcgtgc tgggcgatgt
    22201 ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg cggcaatcgg
    22261 ctcccgctcc gccagacgct ggatttctgc caaccagtga gtgaagcggg tcaatgcttc
    22321 ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct
    22381 tttattgcgc gtcatcgccc actcacccag ctttttcagc gtcgccgggc caatctctcg
    22441 cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg
    22501 cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac caccagaaat
    22561 tttgtacggg atgcggtttt gcatcaggaa tttttcaaac acccgcgact gatagttacc
    22621 gcgataaaga atagcgtaat ctttgtactg cgttttattg acgaagtgat gcgcaatcag
    22681 ttcaccagtg acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa cttttagctc
    22741 tgtgccgtaa cccagttcgg agaacagacg cttttcaaag acgtgcgggt tattggcgat
    22801 caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct caagcttaat
    22861 caccttcagc gccggaaaat cctggctcag cagcaccagg ttttgcggac gtgcgccgcg
    22921 ccaggagtag atcgactggt catcgtcacc caccacggta aagcgcgcgc ggctgcccac
    22981 cagcagtttc accagttcat actggctggt gttggtgtcc tgatattcgt ccaccagcag
    23041 gtagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca gaagtagcgt
    23101 cggcaataaa atcagatcat cgaagtcgag cacattacag gctttcaggt gtgcatcata
    23161 cagcccataa caatgggcaa aaatacggtc ccgctcgcca atcgcactgg ctgccgcctg
    23221 ggacggtgtt ttgagatcgt tcttccagtt agagatggtc gaaatcaact gttgcaggag
    23281 aactttgtca tcttcaatca gcccttcggt caactctttg agcaacgcca gctgatcggt
    23341 atcgtcaaac agcgaaaagt tcgccttcat ccccagcgcc gcatactcac gcttgatgat
    23401 atccagcccc agcgtgtgga aggttgagat catcagccca tgcgcctctt tgcgacccag
    23461 cgtctgtccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg tcaccgccgc
    23521 gatatgtcgc gcctgataac cgcacccgcg gatcagatgg gcgattttat tggtgataac
    23581 acgagtttta ccggaacccg cacccgccag caccaggcag gggccggtaa cgaattcgac
    23641 agcttgttgt tggccggggt ttagacgcat aggaaaatcg ctcaatgaaa gtcgggaggg
    23701 gaaaataaca gcgtggtagt atagccagcc tcatccttac cactcaaggc acgatcatgg
    23761 caaaaacagc agcagcactg catatccttg taaaagaaga gaaactggct ctggatcttc
    23821 tcgtgcagat taagaacggt gccgatttcg gcaagctggc gaagaaacac tccatttgcc
    23881 catcaggcaa acgcggcggt gatttaggtg aattccgcca gggtcagatg gttccggcgt
    23941 tcgataaagt tgtgttctct tgtccggtac tggagccaac cggcccgctg cacacccagt
    24001 tcggttacca catcattaag gtgctgtacc gtaactaata gcaaggcctt ctccgggaga
    24061 aggccttgaa ctctgaatcg ctattaaccc gcaacagcaa tacgtttcat atccgtcata
    24121 tagccgcgca gtttcttacc tacttgctca atcgcatggc tgcgaatcgc ttcatttaca
    24181 tcacgcagct gcgcgttatc taccgcacct tccggaatag ctttacccaa atcgcccggt
    24241 tgcagttctg ccataaacgg tttcagcagc ggcacacaag cgtaagagaa cagatagtta
    24301 ccgtactcgg cagtatcaga gataaccacg ttcatttcat acagacgctt acgggcgatg
    24361 gtgttggcaa tcagcggcag ttcgtgcagt gattcatagt aagcagattc ttcgatgatg
    24421 ccggaatcaa ccatggtttc gaacgccagt tcaacgcccg ctttcaccat cgcgatcatc
    24481 agtacgcctt tatcgaagta ctcctgctcg ccgattttgc cttcatactg cggcgcggtt
    24541 tcgaaggcgg ttttgccggt ctcttcacgc caggtcagca gtttcttatc atcgttggcc
    24601 cagtccgcca tcataccgga ggagaattcg ccggagatga tgtcgtccat atgtttctgg
    24661 aacagcggtg ccatgatctc tttcagctgt tcagacaacg cgtaagcacg cagtttcgcc
    24721 gggttagaga gacggtccat catcagtgtg ataccgccct gcttcagcgc ttcggtgatg
    24781 gtttcccaac cgaactgaat tagtttttct gcgtatgccg gatcggtgcc ctcttccacc
    24841 agcttgtcga agcacagcag cgagccagcc tgcaacatac cgcacaggat ggtttgctca
    24901 cccatcaggt cagatttcac ttccgcaacg aaggacgatt ccagcacgcc cgcacggtga
    24961 ccaccggttg ctgccgccca tgctttagcg atcgccatgc cttcgccttt cggatcgttt
    25021 tccgggtgaa cggcaatcag cgtcggtacg ccgaatccac gtttgtactc ttcacgcact
    25081 tcggtgccag ggcatttcgg cgcaaccatg actacggtga tgtctttacg gatctgctcg
    25141 cccacttcga cgatgttgaa accgtgcgag taacccagcg ccgcgccgtc tttcatcagt
    25201 ggctgtacgg tgcgcactac atcagagtgc tgcttgtccg gcgtcaggtt aaccaccaga
    25261 tccgcctgtg gaatcagttc ttcgtaagta cccactttaa aaccattttc ggtcgcttta
    25321 cgccaggacg cgcgcttctc ggcaatcgct tctttacgca gagcgtagga gatatcgaga
    25381 ccagaatcac gcatgttcag accctggttc agaccttgtg cgccacagcc gacgatgact
    25441 acttttttac cctgaaggta gctcgcgcca tcggcgaatt catcgcgccc cataaagcga
    25501 catttgccca gctgtgccag ctgctgacgc agattcagtg tattgaagta gttagccatg
    25561 gtgattcctc gtgatgttgt gcttcttatt gttcggttcg cgtttgcgag attgaattca
    25621 ctatatgaca ggaaatttat tgcggaaatt gatatattca caacgtcaca ttgcaatttc
    25681 tgcaacgtca acattgaggg ctgcgccatt ggatttacgc gatctgaaaa ccttcctgca
    25741 tctggcggaa agccgccatt ttggccgcag cgcgcgggcg atgcacgtta gcccgtccac
    25801 gctctcacgg cagattcagc gcctggaaga agatctcggc cagccgctgt ttgtgcgcga
    25861 taaccgcacg gtgacgctga ctgaagcggg cgaagaactg cgcgttttcg cccagcaaac
    25921 gctgttgcag tatcagcagc tgcgccacac catcgaccag caagggccgt tgctctatgg
    25981 cgaattacat atcttctgct cggtgaccgc cgcttacagc catctgccgc cgattctgga
    26041 tcgcttccgc gcggaacacc cgtcggtgga gatcaaactc actaccggtg atgcggcaga
    26101 tgcaatggaa aaagtcgtca caggtgaagc tgatctggcg attgccggga agccagagac
    26161 tctgcccggc gcggtggcgt tttcgatgtt ggagaatctg gcagtggtgt tgattgcacc
    26221 cgcattgccc tgcccggtgc gtaatcaggt ttctgtcgaa aagccggact ggtcaacggt
    26281 accgtttatc atggccgatc aggggccggt gcgccgccgc attgagttgt ggttccgacg
    26341 caataaaatc agtaacccga tgatttacgc cacggttggc gggcatgaag cgatggtatc
    26401 gatggtggcg ctcggctgtg gcgtggcatt gttgccggaa gtggtgctgg aaaacagccc
    26461 cgaaccggtg cgtaaccgcg tgatgatttt agagcgcagc gatgagaaaa cgccgtttga
    26521 gctgggcgtg tgtgcgcaga aaaagcggct acatgagccg ctaattgagg cattctggaa
    26581 gattttgccg aaccattaac ccgccaaaaa gaacctgaac gccgggttat tggtttcgtc
    26641 gtggcaatcg tagcccagct cattcagccg ggtttcgaaa tccggttcat ggtcgccaag
    26701 ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt ggaacaaaga
    26761 aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg gtgattccgg
    26821 gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc cgaccatata
    26881 gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt agccgccgtc
    26941 gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc gcacgccgac
    27001 aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga ccgatcgccc
    27061 gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg tcaccgccaa
    27121 caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca ggccgtggaa
    27181 gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg ccgcgaatgt tgtgctgggc
    27241 gatatatttt ttcattcccg ccagcgccag cgcgccagag ggttccgcca ccgcacgcac
    27301 atcttcgaat aaatccttca tcgccgcaca gatcgcatcg ctatcgacgg tgatgatgtc
    27361 gtcgagatac tcctggcata aacggaaggt ttcgtcaccg atgcgtttta ccgcgacacc
    27421 ttcggcgaac agtcccacgc gtggcagatc aaccggatga cccgcatcca gcgctgcttt
    27481 caggcaggcg gagtcttccg cttctacggc gatcactttg atttgcggca tcagttgttt
    27541 gatcagcacc gctacgcccg ccgccagacc gccgccgccg actggcacaa atacgcggtc
    27601 gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg caatcaccat
    27661 cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt cgatcgcttt
    27721 agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga atccacgtac
    27781 cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct tcacgcctaa
    27841 ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag tgatcacgcc
    27901 gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc gcagcttaaa
    27961 gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa gacgcgacga
    28021 cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg gcgcgcgcag
    28081 cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg agtcagccat
    28141 tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct ggttgccagg
    28201 ctggcgtagg cgcacagggc aaaggaaacc tgacgttcac ggtttttcgg cgtccaggct
    28261 ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc gcttacctgt
    28321 aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc aatcagacca
    28381 atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc agaggtgccg
    28441 ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat tgatttcagg
    28501 aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc ttcatagcgg
    28561 atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac cgcgtcgtcc
    28621 tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc gacgcccgcg
    28681 gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag gccgccgtct
    28741 ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc atcgagagaa
    28801 tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg gcctgcgcgg
    28861 aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc cagagtttgc
    28921 ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc gcgatccagt
    28981 tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg gtatttttgg
    29041 gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc gatatcactc
    29101 atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac cgtgttggta
    29161 gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt gctagcaata
    29221 ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa ttcaacaatg
    29281 cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag cagcgagccg
    29341 ttgcccggct gcgacaggcc cagcgcttcg gtcaggcagt tcattgagtt agcggtaaac
    29401 atcccggagc aggagccgca ggtcggacac gcggaacgtt caacctgatc gctctgggag
    29461 tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag tttgattatc
    29521 tgatcggaca gtttggtttt cccggcctcc atcgggccgc cggaaacaaa gatcaccgga
    29581 atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca gttagagatg
    29641 cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc agcgatcagt
    29701 tcgcgagatg gaagtgaata aagcatcccc ccgtggccca tggcaatccc atcatccacc
    29761 gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc ggcgaccagt
    29821 ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga gttcacaacc
    29881 gcgataatcg gcttgccaaa atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga
    29941 gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg catactttat
    30001 ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt tttgtattta
    30061 ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga agaggccgaa
    30121 gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg ccttcgccaa tctgaatacc
    30181 gtctacgctg cgcactggcg tgatttctgc cgccgtaccg gacataaaga cttcatccgc
    30241 caagtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca gctctttcgc
    30301 cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga acggtggggt
    30361 gaacagcaca ccatctttca cttcaaacag gttttcgcct gcgccttcag agatataacc
    30421 gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc tacccaccag
    30481 cagggaagag agatagttac caccggcttt tgccgcagtt gggatggtgt ttggtgctgc
    30541 gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg cgcccagata
    30601 cgctccccac gggaaagcgg cgataatcac atcggttgag tatcccgctg gcgggtttac
    30661 tcccatgcca acattaccga cgaagatcag cggacggata taggcgctgg tgagattgtt
    30721 tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgtg aaaccgggaa
    30781 gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc ggaatacaac
    30841 cggtcctttg tgtgagtcgt agcaacggat gccttcaaaa accgaggtgc catagtgcag
    30901 cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc cattgaacca
    30961 aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag gcgcggattt
    31021 gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc agtttattta
    31081 actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg atatttatat
    31141 tttgtgcatc gctggcggca gccatattca ttgagcagac gtggaaacca cgatgacgca
    31201 ccacgcgtaa aacacgttct aaggtttccg gattgaagcg agccgataca ttgacctgat
    31261 gttgcatcat gataatttct ccaacatttc tgaattactg gcacctggcg gcaccagcgg
    31321 ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat cactgttcag
    31381 catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc cagggatgcc
    31441 gaaggcgctg gctaacatga ggaaatcggg gttatcggta agggtggttt cgctgtatcg
    31501 ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt tatcgagtaa
    31561 gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca cattcatcat
    31621 gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga cttgtgcgcc
    31681 aactgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga tgaaattttc
    31741 cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca catctgtggt
    31801 cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg gagcgtagat
    31861 agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg cgcagtgttg
    31921 ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat ttaaatcacc
    31981 ttgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga tatccatatg
    32041 gataacactg gcgtgtggtg cgaaggtgtt cagtttgccg gtcacccggt catcaaaacg
    32101 tgcacccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg ctttggttcc
    32161 gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc ccagcccttt
    32221 cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag ccggaactgc
    32281 ctgcgccatc cccacgccac cgccaacgta cagcatcggt ttttgcgctt ttgccagcat
    32341 ctggcgcgct tgctcgactt cggcatgtgg gaaagtcact tcgttttcaa cggtggtgaa
    32401 ccacggttcc agatcgccgc tggctaactg gatatctttt gggatatcga ccagaaccgg
    32461 accaggacga cctgagctgg caacgtcgaa tgcttcagcc atgatgcgcg gcaactcttc
    32521 cagagactgc accagaaagc tgtgcttggt acaggctaac gacaatccca ggacatccac
    32581 ttcctgaaat gcgtcggtgc cgataaacgg tgcggacacc tgaccagtga tggcaacgac
    32641 agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg cgcccggacc
    32701 agacgtggcg atacatacgc cagttttacc ggtagcacgg gcataaccga tagccgccat
    32761 tgccgcaccc tgctcatgtc ggcacagcaa gtgctccacg ccgccgtcat acaatgcatc
    32821 gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac cctgtgcccg
    32881 caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct gaatcttgag
    32941 aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt aaggtcaaaa
    33001 aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc tctaagcctt
    33061 tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc taatcacgac
    33121 caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt gttcgaagga
    33181 atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg gcctattttt
    33241 atgatggctt taataatttc aatagaaatt gttgtttttt aaaaagaaaa tagtgagtga
    33301 aaaaattcaa tttcagtata aatgttgttc ccttcctcgt agttcatcct ttgtccgctg
    33361 catttttgcg agcatcattc caccgccaga acaactcaac ttcacttgca caaataaaca
    33421 ggaatcagcc actaaaattc acgaattagc gggctacagc agcgatatct tatgcacata
    33481 atggtgactc aaggagggtt tatgtcactg tcaattgttc atacccgcgc agccctggaa
    33541 gtaaatgcgc ccccaatcac tgttgaggta catatcagta aaggtctacc cggcttaacg
    33601 atggtgggct taacagaaac aacggtgaaa gaagctcgcg atcgcgtgcg cagcgccatt
    33661 atcaatagcg gatatgaata tccggcgaaa aaaatcacca tcaaccttgc tccagccgat
    33721 ctgccaaaag aagggggacg atatgattta cctatcgcca ttgcgttgct ggcggcctca
    33781 gaacagctta cagccaataa gttagatgaa tatgaattag tcggagaact agcgcttaca
    33841 ggcgctctgc gtggcgttcc cggtgcaatc tccagtgcaa ctgaagctat taagtcgggc
    33901 agaaaaatta tcgtcgcgaa agataacgaa gatgaagtgg ggctaattaa cggtgaagga
    33961 tgcctgatag ccgatcatct gcaggctgtc tgtgcgtttc tggaaggtaa gaacactctc
    34021 gaacgcccga aaccaactga tgcagtatcc cgggcgctac aacatgatct cagtgatgtt
    34081 gtcggtcagg agcaaggaaa acgaggactg gaaattaccg ccgctggtgg gcacaacctt
    34141 ttactgattg ggccgccggg aacaggtaaa acaatgctcg ccagccgtat taatggcctg
    34201 ttgccagatt taagcaatga agaggcactg gagagcgctg cgatattaag tctggtaaat
    34261 gctgaatcag tacaaaaaca atggcggcag cgcccgttcc gctcacctca tcacagtgcg
    34321 tcgttaactg cgatggtggg cggtggtgca attccagggc caggtgaaat ttcgttggcg
    34381 cataacggcg tgctttttct tgatgagcta cctgaatttg aacggcgtac actggatgcc
    34441 ttgcgagagc cgattgaatc cgggcagatc catctttcac gcacacgagc aaaaataacc
    34501 tatccagccc gtttccagct tgttgcggcg atgaatccca gccctaccgg acattatcag
    34561 ggaaaccata accgctgcac gccagaacag acattacgtt atctcaaccg gctctcgggg
    34621 ccctttctcg accgcttcga tctctcactg gagatcccat taccaccccc cggcattttg
    34681 agtaaaacgg tagtgccggg agaaagcagc gccaccgtta aacaacgcgt aatggccgcc
    34741 agagagcgcc aatttaagcg gcagaataaa ctgaatgcct ggctggatag tccggaaata
    34801 cgccaattct gcaaacttga gagcgaagat gcgatgtggt tggaagaaac gctgatccat
    34861 ctggggttat cgattcgtgc ctggcagcgg ttactgaaag ttgcacgaac cattgctgat
    34921 attgatcagt ctgacattat cacacgtcag catttgcagg aggcagttag ctatcgtgcg
    34981 attgaccgtt tgctcatcca tctgcaaaaa ctactgacat aaaaaaaggg catttcgccc
    35041 tttttattaa tcgtcagaat cggtgtagtc ttcagcacct tcaacctgcg gtttaccgcc
    35101 ggaaagggtg tgaaaacgtt ttggacgctt aatacgcgtc atatacttgg accacacgcg
    35161 ttctgcttct gtcactggct cacgttcgcc acggcatact gctacgaaga gtttctcttc
    35221 ctcggtaacc ggctcgcgtt tgccaagatc caactcattg aaggcataac catgacgctc
    35281 aagcagttgt gcctctttga tggtgaaatc accatgacga gagaatccac gtggataatg
    35341 tttattgtcg aaatatcgat tagtcgtcgt aaagctttcc gccatcctgc acgctcctaa
    35401 ttctttgacc gagctagtta tggcgcggag tattagttac gcttgacaga gtgtaaaaca
    35461 aaacatttaa atcataacga caaataattt tgcggagagc actgtggata cggaattgtt
    35521 aaaaactttc ctggaagtta gccgaacgcg tcactttggt cgagcggctg aatcgctcta
    35581 tctgacccag tcagcagtga gctttcgaat cagacaactg gaaaatcaac tgggtgtgaa
    35641 ccttttcacc cgccacagaa acaatatccg tttaaccgct gccggtgaaa aactactgcc
    35701 ttatgcagaa acgctcatga gcacctggca ggccgcccgc aaggaggtgg cgcatacctc
    35761 acgacataac gagttttcta tcggtgccag cgcctcgttg tgggaatgta tgcttaatca
    35821 gtggttggga cgcttgtatc aaaatcagga tgcccataca ggcttacagt tcgaagcgcg
    35881 aattgcccaa cggcagtctc tggtaaaaca gctgcatgaa cgccagcttg atcttcttat
    35941 taccactgaa gcgcccaaaa tggacgaatt tagtagtcag ttgctggggt atttcacttt
    36001 agcgctttat accagtgccc cttctaaact aaagggagat cttaactatc tgcgactgga
    36061 gtgggggcca gattttcaac agcatgaggc aggtttgatc ggtgctgacg aagttcccat
    36121 tctgacaacc agttctgctg aactggcaca gcaacagatt gcgatgctta atggttgcac
    36181 ctggctaccc gtcagctggg cgcgtaaaaa aggcggcctg cataccgttg tcgatagcac
    36241 aacactttca cggccgcttt atgccatatg gctgcaaaat agcgataaaa atgcgttgat
    36301 tcgcgatctt ttgaaaatta acgtgctgga tgaagtgtat taatctgaat ggctggcaag
    36361 gatgccggta gaaggattta cttcggagag gattatttca gataaaaaaa atccttagct
    36421 ttcgctaagg atgatttctg gcaggggcgg agagactcga actcccaaca cccggttttg
    36481 gagaccggtg ctctaccaat tgaactacgc ccctaattag
//
LOCUS       DAEEUE010000032        35269 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.032, whole genome shotgun sequence.
ACCESSION   DAEEUE010000032 DAEEUE010000000
VERSION     DAEEUE010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35269
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.032"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..1085)
                     /locus_tag="KLK84_001731"
     CDS             complement(<1..1085)
                     /locus_tag="KLK84_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBE2009091.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSSPDYH
                     GPRIYFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEEHANTFSAFLDR
                     LSDTVSARNTSGFREQVAAWLEKLSASA"
     gene            1177..1263
                     /locus_tag="KLK84_001732"
                     /pseudo
     CDS             1177..1263
                     /locus_tag="KLK84_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1668..2144)
                     /locus_tag="KLK84_001733"
     CDS             complement(1668..2144)
                     /locus_tag="KLK84_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HBE2009092.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(2203..3492)
                     /gene="bioA"
                     /locus_tag="KLK84_001734"
     CDS             complement(2203..3492)
                     /gene="bioA"
                     /locus_tag="KLK84_001734"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HBE2009093.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3579..4619
                     /gene="bioB"
                     /locus_tag="KLK84_001735"
     CDS             3579..4619
                     /gene="bioB"
                     /locus_tag="KLK84_001735"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBE2009094.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4616..5770
                     /gene="bioF"
                     /locus_tag="KLK84_001736"
     CDS             4616..5770
                     /gene="bioF"
                     /locus_tag="KLK84_001736"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HBE2009095.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5757..6512
                     /gene="bioC"
                     /locus_tag="KLK84_001737"
     CDS             5757..6512
                     /gene="bioC"
                     /locus_tag="KLK84_001737"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HBE2009096.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6505..7182
                     /gene="bioD"
                     /locus_tag="KLK84_001738"
     CDS             6505..7182
                     /gene="bioD"
                     /locus_tag="KLK84_001738"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBE2009097.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7761..9782
                     /gene="uvrB"
                     /locus_tag="KLK84_001739"
     CDS             7761..9782
                     /gene="uvrB"
                     /locus_tag="KLK84_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HBE2009098.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLAEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9974..10882)
                     /gene="yvcK"
                     /locus_tag="KLK84_001740"
     CDS             complement(9974..10882)
                     /gene="yvcK"
                     /locus_tag="KLK84_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HBE2009099.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      11147..11291
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11279..12268
                     /gene="moaA"
                     /locus_tag="KLK84_001741"
     CDS             11279..12268
                     /gene="moaA"
                     /locus_tag="KLK84_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBE2009100.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12290..12802
                     /gene="moaB"
                     /locus_tag="KLK84_001742"
     CDS             12290..12802
                     /gene="moaB"
                     /locus_tag="KLK84_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HBE2009101.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12805..13290
                     /gene="moaC"
                     /locus_tag="KLK84_001743"
     CDS             12805..13290
                     /gene="moaC"
                     /locus_tag="KLK84_001743"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBE2009102.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13283..13528
                     /gene="moaD"
                     /locus_tag="KLK84_001744"
     CDS             13283..13528
                     /gene="moaD"
                     /locus_tag="KLK84_001744"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HBE2009103.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13530..13982
                     /gene="moaE"
                     /locus_tag="KLK84_001745"
     CDS             13530..13982
                     /gene="moaE"
                     /locus_tag="KLK84_001745"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HBE2009104.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            14119..14823
                     /locus_tag="KLK84_001746"
     CDS             14119..14823
                     /locus_tag="KLK84_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBE2009105.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            15028..15741
                     /locus_tag="KLK84_001747"
     CDS             15028..15741
                     /locus_tag="KLK84_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBE2009106.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLEQAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15777..16733)
                     /locus_tag="KLK84_001748"
     CDS             complement(15777..16733)
                     /locus_tag="KLK84_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HBE2009107.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16733..17974)
                     /gene="clsB"
                     /locus_tag="KLK84_001749"
     CDS             complement(16733..17974)
                     /gene="clsB"
                     /locus_tag="KLK84_001749"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HBE2009108.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGIQVFEYRRRPLHGKVALMVDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17971..18732)
                     /locus_tag="KLK84_001750"
     CDS             complement(17971..18732)
                     /locus_tag="KLK84_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBE2009109.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADIMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18865..19275
                     /locus_tag="KLK84_001751"
     CDS             18865..19275
                     /locus_tag="KLK84_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HBE2009110.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(19237..20343)
                     /locus_tag="KLK84_001752"
     CDS             complement(19237..20343)
                     /locus_tag="KLK84_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE2009111.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20354..21487)
                     /locus_tag="KLK84_001753"
     CDS             complement(20354..21487)
                     /locus_tag="KLK84_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE2009112.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21480..23216)
                     /locus_tag="KLK84_001754"
     CDS             complement(21480..23216)
                     /locus_tag="KLK84_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE2009113.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(23209..24204)
                     /gene="hlyD"
                     /locus_tag="KLK84_001755"
     CDS             complement(23209..24204)
                     /gene="hlyD"
                     /locus_tag="KLK84_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HBE2009114.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYNLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(24207..24878)
                     /gene="cecR"
                     /locus_tag="KLK84_001756"
     CDS             complement(24207..24878)
                     /gene="cecR"
                     /locus_tag="KLK84_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HBE2009115.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            25107..26294
                     /gene="rhlE"
                     /locus_tag="KLK84_001757"
                     /pseudo
     CDS             25107..26294
                     /gene="rhlE"
                     /locus_tag="KLK84_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(26501..26983)
                     /gene="ybiA"
                     /locus_tag="KLK84_001758"
     CDS             complement(26501..26983)
                     /gene="ybiA"
                     /locus_tag="KLK84_001758"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HBE2009116.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            27103..29253
                     /gene="dinG"
                     /locus_tag="KLK84_001759"
     CDS             27103..29253
                     /gene="dinG"
                     /locus_tag="KLK84_001759"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HBE2009117.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            29281..30243
                     /gene="ybiB"
                     /locus_tag="KLK84_001760"
     CDS             29281..30243
                     /gene="ybiB"
                     /locus_tag="KLK84_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HBE2009118.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30384..31469
                     /gene="hcxB"
                     /locus_tag="KLK84_001761"
     CDS             30384..31469
                     /gene="hcxB"
                     /locus_tag="KLK84_001761"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HBE2009119.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(31698..31958)
                     /locus_tag="KLK84_001762"
     CDS             complement(31698..31958)
                     /locus_tag="KLK84_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBE2009120.1"
                     /translation="MKTINTVVAAMALSTLSFGVFATEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(32223..32489)
                     /gene="ybiI"
                     /locus_tag="KLK84_001763"
     CDS             complement(32223..32489)
                     /gene="ybiI"
                     /locus_tag="KLK84_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HBE2009121.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32563..33240)
                     /gene="ybiX"
                     /locus_tag="KLK84_001764"
     CDS             complement(32563..33240)
                     /gene="ybiX"
                     /locus_tag="KLK84_001764"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HBE2009122.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(33282..35269)
                     /locus_tag="KLK84_001765"
                     /pseudo
     CDS             complement(33282..35269)
                     /locus_tag="KLK84_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8356 a         9033 c         9542 g         8338 t
ORIGIN      
        1 gcagaggcac tgagtttttc cagccatgca gcgacctgtt cacggaatcc ggaggtattg
       61 cgtgcagaga cggtatcgga aaggcggtca aggaacgcgg aaaaggtgtt ggcgtgctct
      121 tcatgttcaa aagcatgcca tatctgtgat acatcagatt gtttgttttc cgggaaccat
      181 gctgtcacgg catcagccag ggggcgatgg agtgtattct gttgtccgtc actcatggag
      241 aagtaaatcc gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga
      301 gtgcgagtcg acaatggatt gttataaata ttaacatctg cataaatgtg cagattcatg
      361 atactgtctg gcaatgtgtt cagctgattg ttagggatat atagtgattg cagacgttgt
      421 ggaagttcgg gcagtctgtg tagttggttt tccccaacgt ttaaaagcct cagattttga
      481 ggaaggggcg gtagctcttg taatctgtta tcaaaagcac ttagttcctg tagttcacat
      541 ggtaattctg gtaaggcagt aagctggttg ttgccaacag aaagtttttg tagagccggg
      601 ggaagatcag gtagcgtctc caggctattg agaagggctg agagtgactg caaggaagat
      661 ggaagagaag gcaaacatgt taaaactctg ttaagtgaaa catcaagagc gaccaggtga
      721 ggcggaagag ccgggagcct gctcagtctg ttatcgctgg ctttaagtac agttaaggac
      781 ggagggagtt ctggcagaga gcgtagctca ttgctagaga tgttaagttc ttgtatgtgc
      841 gggggcaggt atgggagaga gcgtaatctg agtaaactta aattgagggc tggctcttga
      901 aaagccagac atattttcag taatcgaact gcctgtgttc ggtcttctgt tgcagcacct
      961 tccttggccc agttatccca gatgcggtga taatccgtga tattttgctc ttctacggat
     1021 aaacgggata tacatcctaa accacaaaag gagcggaaaa aagaaggatt gtgggcaggt
     1081 ttcatttatt attgatcctc attgtttata tattaatata ttcgtgcaaa tggagtgagg
     1141 attctttaaa aaaacgcgcc tgtaaatagt taagaacagg aagaactgtt ggggatgtgg
     1201 ctggccgaaa atgaaggggt gaagttctgg ctcaatgtgc tgactgaact gaaaaaacgc
     1261 ggttggtcgc cctacctttt catcaagcca gtagtctgcc caccacagca tcatttctct
     1321 gcgcttatcg agatactgag catggttgta aattcagcta acccagtgcg ttgacaaaaa
     1381 attagcgcaa gaggacaaaa aatcaccttg cgctaatgct ctgtatcagg tcactaatac
     1441 catctaagtg gttgattcat agtgactgga tatgttgtgt tttgcagtat tatgcagtct
     1501 attttttaag ctaaatgtat tataatgcat tgatattaat gatttttact gtttctcggt
     1561 cagctttttt atactaactt gagcgaaacg ggaaggtaaa aagacaaaaa gttgttttta
     1621 atacctttaa gtgataccag atggcattgc gccatctggc agagtgatta actaaacatc
     1681 gcggtaatcg atgcgcttgc cagagagtgg aaatgaacgt taaacccgac catcgcgccg
     1741 ctggctcctt catcgacatc aatacgctct acatccagcg cgtgaacggt aaaaatgtag
     1801 cgatgggttt cgcctttcgg cggcgctgcg ccatcgtatc cggttttacc aaaatcggta
     1861 cgcgtctgca aaacgccgtc tggcattgct accagaccag agccaaaccc ttgcggtaat
     1921 acgcgggtat cagcgggtaa attaacaact acccagtgcc accagccgga gccggttggc
     1981 gcatccgggt cgtagcaggt gacaacaaaa cttttcgttc ccgcaggaac atcatcccac
     2041 gccagatgcg gtgaaatatt atcgccatcg taacccatgc cgttaaagac atgacgatgc
     2101 ggcaacttat cgccatcgcg cagatcgtta ctgatgagtt tcatgaaccc tcctttcttg
     2161 tttgcagaaa gtgtagccag aaaccctcac gctgacttcc cgttattggc aaaaaaatgt
     2221 ttcatcctgt accgcgcggt taaccgctgc ggtcagacgc tgcaactgtt gcgggagaat
     2281 aatatagggc ggcatcaggt aaatcagttt gccaaaaggc cggatccata ccccctgttc
     2341 gacaaagaat ttttgcagcg ccgccatatt caccggacga gtggtttcga ccacaccgat
     2401 tgcccccagt acgcgcacat cggcaaccat ttcggcatca cgggctggtg ccagttgctc
     2461 gcgcagctgc acttcaatag ccgccacctg ctgctgccat tcgccggatt cgataatcgc
     2521 taggctggcg tttgctgccg cgcaggccag cggattgccc ataaaagttg gcccatgcat
     2581 aaagcagccg gcttcgccgt tactgatggt ttctgcaacc tcgcgcgtgg taagtgtggc
     2641 ggaaagggtc attgtgccgc cggttaaggc tttaccgagg cacaaaatgt ccggcgcgat
     2701 ttctgcatat tcacaggcaa acagtttgcc ggtacgacca aatccggtgg cgatctcgtc
     2761 ggcaatcagc aagatacctt cgcgatcgca cattttgcgg attcgtttta accattccgg
     2821 atggtacatg cgcatcccgc ctgcgccctg gacaatcggc tcaatgatca ccgctgcgat
     2881 ttcatgacga tgcgccgcca tcaggcgggc aaaaccaacc atatcgcgtt catcccattc
     2941 accatccatg cggctttgcg gggcgggagc aaacaagttt tctggcagat agcctttcca
     3001 caggctgtgc attgagttgt ccggatcgca caccgacatc gcgccaaagg tatcgccatg
     3061 ataaccattg cggaaggtca gaaaacgctg gcgcgcttcg cctttggctt gccagtactg
     3121 caacgccatt ttcatcgcca cttccaccgc tacggaaccg gagtccgcga gaaaaacgca
     3181 ctccagcggt tgcggcgtca ttgccaccag tttgcggcat agctcgatgg cgggcgcatg
     3241 ggtgataccg ccaaacatca catgcgacat ggcatcaatt tgcgtcttca tcgccgcatt
     3301 aagctgcggg tgattgtagc cgtggatcgc cgcccaccag gacgacatac cgtcaaccag
     3361 gcgtctgccg tcagacaaaa ttagctcgca accttcggcg ctcgccaccg gataaaccgg
     3421 cagaggggag gtcatggatg tgtatgggtg ccagatatgg cgttggtcaa aggcaagatc
     3481 gtccgttgtc ataatcgact tgtaaaccaa attgaaaaga tttaggttta caagcctaca
     3541 ccgaattaac aacaaaaaac acgttttgga gaagccccat ggctcaccgc ccacgctgga
     3601 cattgtcgca agtcacagaa ttatttgaaa aaccgttgct ggatctgctg tttgaagcgc
     3661 agcaggtgca tcgccagcat ttcgaacctc gtcaggtgca ggtcagcacg ttgctgtcga
     3721 ttaagaccgg agcttgtccg gaagattgca aatactgccc gcaaagctcg cgctacaaaa
     3781 ccgggctgga agccgagcgg ttgatggaag ttgaacaggt gctggagtcg gcgcgcaaag
     3841 cgaaagcggc aggatcgacg cgcttctgta tgggcgcggc atggaagaat ccccacgaac
     3901 gcgatatgcc gtacctggaa caaatggtgc agggggtaaa agcgatgggg ctggaggcgt
     3961 gtatgacgct gggcacgttg agtgaatctc aggcgcagcg cctcgcgaac gccgggctgg
     4021 attactacaa ccacaacctg gacacctcgc cggagtttta cggcaatatc atcaccacac
     4081 gcacttatca ggaacgcctc gatacgctgg aaaaagtgcg cgatgccggg atcaaagtct
     4141 gctcaggtgg cattgtgggc ttaggcgaaa cggtaaaaga tcgcgccgga ttattgctgc
     4201 aactggcaaa cctgccgacg ccgccggaaa gcgtaccaat caacatgctg gtgaaggtga
     4261 aaggcacgcc gcttgccgat aacgatgacg ttgatgcctt tgattttatt cgcaccattg
     4321 ccgtagcgcg gatcatgatg ccgacctctt acgtgcgcct ttctgccgga cgcgagcaga
     4381 tgaacgaaca gactcaggcg atgtgcttta tggcaggcgc aaactcgatt ttctacggtt
     4441 gcaaactgct gaccacgccg aatccggaag aagataaaga cctgcaactg ttccgcaaac
     4501 tggggctaaa tccgcagcaa actgccgtgc tggcagggga taacgaacaa cagcaacgtc
     4561 ttggacaggc gctgatgacc ccggacaccg acgaatatta caacgcggca gcattatgag
     4621 ctggcaggag aaaatcaacg cggcgctcga tgcgcggcgt gctgccgatg ccctgcgtcg
     4681 ccgttatccg gtggcgcaag gagccggacg ctggctggtg gcggatgatc gccagtatct
     4741 gaacttttcc agtaacgatt atttaggttt aagccatcat ccgcaaatta tccgtgcctg
     4801 gaagcagggg gcggagcaat ttggcatcgg tagcggcggc tccggtcacg tcagcggtta
     4861 tagcgtggtg catcaggcgc tggaagaaga actggccgag tggcttggct attcgcgggc
     4921 gctgctgttt atctctggtt ttgccgcgaa tcaggcagtc attgccgcga tgatggcgaa
     4981 agaggaccgt attgttgccg accggcttag ccatgcctca ttgctggagg ctgccagttt
     5041 aagcccgtcg cagcttcgcc gttttactca taacgatgtc gctcatctgg cgcgactgct
     5101 tgcttccccc tgtccggggc agcaactggt ggtgacagaa ggcgtgttca gcatggacgg
     5161 cgatagcgcg ccactggcgg aaatccagca ggtaacgcaa cagcacaatg gctggttgat
     5221 ggtcgatgat gcccacggca cgggcgttat cggggagcag gggcgcggca gctgctggct
     5281 gcaaaaggta aaaccagaat tgctggtagt gacttttggc aaaggatttg gcgtcagtgg
     5341 ggctgcggtg ctttgctcaa gtacggtggc ggattatctg ctgcaattcg cccgccacct
     5401 tatctacagc accagtatgc cgcccgctca ggcgcaggca ttacgtgcgt cgctggcggt
     5461 cattcgccgt gatgaggggg atgcacggcg cgaaaaactg gtgtcactca ttgcgcgttt
     5521 tcgtgccgga gtacaggatt tgccgtttac gctggctgat tcatgcagcg ccatccagcc
     5581 attgatcgtc ggtgataaca gccgtgcgtt acaactggca gaaaaactgc gccagcaagg
     5641 ctgctgggtc acggcgattc gcccgccaac cgtacccgct ggtactgcgc gactgcgctt
     5701 aacgctaacc gctgcgcatg aaatgcagga tatcgaccgt ctgctggagg tgctgcatgg
     5761 caacggttaa taaacaagcc attgcagcgg catttggtcg ggcagccgca cactatgagc
     5821 aacatgcaga tctacagcgc cagagtgctg acgccttact ggcaatgctt ccacagcgta
     5881 aatacaccca cgtactggac gcgggttgtg gacctggctg gatgagccgc cactggcggg
     5941 aacgtcacgc gcaggtgacg gcgttagatc tctcgccgcc aatgcttgcg caagcccgcc
     6001 ataaagatgc cgcagatcat tatctggcag gagatatcga atccctgccg ttagcgactg
     6061 cgacgttcga tcttgcatgg agcaatctcg cggtgcagtg gtgcggtaat ttatccacgg
     6121 cactccgcga gctgtatcgg gtggtgcgcc ccggaggcgt ggtcgcgttt accacgctgg
     6181 tgcagggatc gttacccgaa ctgcatcagg cgcggcaggc ggtggacgag cgtccgcatg
     6241 ctaatcgctt tttaccgcca gatgaaatcg aagagtcgct gaacggcgtg cgttatcaac
     6301 atcatattca gctcatcaca ctatggtttg atgatgcgct cagtgccatg cgttcgctga
     6361 aaggcatcgg tgctacgcat cttcatgaag ggcgcgaccc gcgaatatta acgcgttcgc
     6421 agttgcagcg attgcaactg gcctggccgc aacaacaggg gcgatatcct ctgacgtatc
     6481 atcttttttt gggagtgatt gctcgtgagt aaacgttatt ttgtcaccgg aacggatacc
     6541 gaagtgggga aaaccgtcgc cagttgtgca cttttacaag ccgcgaaggc agtaggttat
     6601 cggacggcag gttataaacc ggtcgcctct ggcagcgaaa agaccccgga aggcttacgt
     6661 aatagtgacg cgctggcgtt acagcacaac agcagcctgc agctggatta cgcaacggta
     6721 aatccttaca ccttcgcaga acccacctcg ccgcacatca tcagcgccca agagggcagg
     6781 ccgatagaat cattggtaat gagcgccgga ttacgcgcgc ttgaacaaca ggctgactgg
     6841 gtgttagtgg aaggtgctgg cggctggttt acgccgcttt ctgacacttt cacttttgca
     6901 gattgggtaa cacaggaaca actgccggtg atactggtag ttggtgtgaa actcggctgt
     6961 attaatcacg cgatgttgac tgcgcaggca atacaacacg ccggactgac tctggcgggt
     7021 tgggtggcga acgatgttac gcctccggga aaacgtcacg ctgaatatat gaccacgctc
     7081 acccgcatga ttcccgcgcc gctgctggga gagatcccct ggcttgcaga aaatccagaa
     7141 aatgcggcaa ccggaaagta cataaacctt gccttgttgt agccattctg tatttggtta
     7201 aattgcgagc gagatcgcgt cttcgattga ctgcaattta accaattaaa ttctaaaata
     7261 atcacgaaaa aaattttact tccgcctcat gcggcgaatg tgggaattgc ccaggcggcg
     7321 ggggataggg gctggagaca gttatccact attcctgtgg ataaccatgt gtattagagt
     7381 tagaaaacac gaggcaagcg agagaatacg cggcttgcac gcgaattggc gttaaagacg
     7441 gctcaaagaa atatctttta ttttttaact ggttagataa atgcaatggc agtcactgaa
     7501 caggcatctc ttgccataaa actgtcatca ctcatcttga caaatgttaa aaaagccgtt
     7561 gctttgggga taacccggta aggctggagt tttatctcgc cacagagtaa attttgctca
     7621 tgattgacag cggagtttac gctgtatcag aaatattatg gtgatgaact gtttttttat
     7681 ccagtataat ttgttggcat aattaagtac gacgagtaaa attacatacc tgcccgccca
     7741 actccttcag gtagcgactc atgagtaaac cgttcaaact gaattccgct tttaaacctt
     7801 ctggcgatca gccagaggcg attcgacgtc tcgaagaggg gctggaagat ggcctagcgc
     7861 accagacgtt acttggcgtg actggctccg ggaaaacctt caccattgcc aatgtcattg
     7921 ctgaccttca gcgcccaacc atggtacttg cgcccaacaa aacgctggcg gcccagctgt
     7981 atggcgaaat gaaagagttc ttcccggaaa acgcggtgga gtatttcgtc tcctactacg
     8041 actactatca gccggaagcc tatgtaccga gttccgacac cttcattgag aaagatgcct
     8101 cggttaacga acatattgag cagatgcgtt tgtccgccac caaagcgatg ctggagcggc
     8161 gtgatgtgat tgtagtggcg tctgtttccg cgatttatgg tctgggcgat cctgatttat
     8221 atctcaagat gatgctccat ctcacggtcg gtatgattat cgatcagcgc gcgattctgc
     8281 gccgactggc ggagctgcaa tacgctcgta atgatcaagc attccagcgt ggtactttcc
     8341 gcgttcgtgg cgaggtgata gatatcttcc cggcagaatc ggatgacatt gcacttcgcg
     8401 tggaactgtt tgacgaggaa gtggaacgat tgtcgttatt tgacccgctg accgggcaga
     8461 ttgtttccac tattccacgt tttaccatct acccgaaaac gcactacgtc acaccgcgcg
     8521 agcgcatcgt acaggcgatg gaggagatca aagaagaact ggccgccaga cgcaaagtgc
     8581 tgttggaaaa caacaaactg ctggaagagc agcggctgac ccagcgtacc cagtttgatc
     8641 tggagatgat ggacgagctg ggctactgtt cggggattga aaactactcg cgcttcctct
     8701 ccggtcgtgg accgggtgag ccaccgccga cgctgtttga ttacctgcct gccgatgggc
     8761 tgctggtcgt cgatgaatct cacgtcacca ttccacaaat tggcggcatg tatcgcggtg
     8821 accgggcgcg taaagagaca ctggtggagt acggcttccg cctgccatca gcgctggata
     8881 accgtccgct taagtttgaa gagttcgaag cattagcgcc gcaaaccatc tatgtttcgg
     8941 cgacgccggg taattacgag ctggaaaaat ccggcggcga tgtggtggat caggtggtgc
     9001 gtccaaccgg attgcttgac ccgattatcg aagtgcggcc ggtggcgaca caggttgatg
     9061 atcttctttc ggagattcgt cagcgagcgg caattaacga acgcgtgctg gtcaccacac
     9121 tgaccaagcg aatggcggaa gatcttgccg aatatctcga agaacacggt gagcgcgtgc
     9181 gttatcttca ctcagatatc gacaccgtcg agcgtatgga gattatccgc gacttgcgtc
     9241 tgggtgagtt cgacgtattg gtagggatca acttactgcg tgaaggtctg gatatgccgg
     9301 aagtgtcgct ggtggcgatc ctcgacgctg acaaagaagg cttcctgcgt tccgaacgtt
     9361 cgttgatcca gaccattggt cgtgcggcac gtaacgttaa cggtaaagcg attctctacg
     9421 gcgataggag cactccatca atggcgaaag cgattggcga aaccgaacgt cgccgtgaga
     9481 aacagcagaa gtacaacgag gaacacggca ttacgccgca aggcttgaac aagaaagtgg
     9541 tcgatatcct ggcgctgggg cagaacattg ccaaaaccaa agcgaagggc agaggaaaat
     9601 cgcgcccgat tgttgagccg gataatgtgc cgatggatat gttgccgaaa gcgttgcagc
     9661 agaaaatcca tgaactggaa gggctgatga tgcaacacgc gcagaatctg gagttcgaag
     9721 aagcggcaca aattcgtgac cagttgcatc agttgcgtga gttgtttatt gccgcatcgt
     9781 aacaggatag cgaagaagac tgatgacaaa cggaaaacag tctgatgcgc tacgcttatc
     9841 aggcctacat tttctccgca atatattgaa tttgcgcggt ttgtaggccg gataaggcgt
     9901 tcacgccgca tccggcataa acaacgcgca ctttgtcttc aataggaagc cggaattttc
     9961 cttcggattt ccgttaacct aaagcctgta acgccttttc cagcgcgtta tgtaacaact
    10021 ggcggtcatg acgatacgga atatcgctgg cctccagtac ctcctggatc acaatccgct
    10081 ctttcaccgc cgagacatcc acttttggcc cgacgatgac cgcatcaatg acctttttac
    10141 caacatactg ctccataatt gccagcttgc tttccagctt caaattagcc gcaggtaaac
    10201 tcaactcacg ccccagattg ccgatataaa ccatcggcgc tggcgtgcgg cgtaatgcct
    10261 gggcgatttc cttcagcagc agaattggca tcaggctggt ataaaaactg ccaggcccaa
    10321 taatgatgag atccgcttca ttgatagcgt gaaccgcctc acgcgttgcg ggtacattag
    10381 gcgttaacaa taactcttga atcggcgtag ttaactggtc gatattgacc tcgccgtaaa
    10441 cttcatgccc ctgatcgtca atcgccatca gatcaacagg atgctctgac attggaatca
    10501 aatgcgtatc cactttcagc agattacgaa ttaaattgat ggcttccaga ggccgcacgc
    10561 taaggtgatc cagcgccttt aacatcaagt ttccgagatt atgaccggaa agttcgccat
    10621 tgccgccaaa acggtattca aacatcgcgg aggcgacgtt cggttccgtt atcagctggt
    10681 tgaggcagtt gcgcatatcg ccccaagcaa tgccgccttc tgaacggcga atacgccccg
    10741 tcgagccacc attatccgtg gtggtgacga tacccgttaa acgagaaccc aaagacgaaa
    10801 gtgatgagag aacgcgtccc agtccatgcc ctccgccgag agcaacgaca cgatcaagat
    10861 cagccagcgt acgattgcgc atatagtttc ctggagtcag attatccgcg ctacagtagc
    10921 gcaaattgcg gggaaacagc aattaacctg ccaatattga ggatacaaac tattttcttc
    10981 tatctctaaa ggacgatgca cgctatgcct ccctgatgat gtatatcaaa gtaaaaccgc
    11041 cattttccct tattctgtag cgaaatagca cgatcatgac gctatataca tgattacata
    11101 gcgaaagtgt ggatggtaaa aatctcattt acacgctagt atcggcttaa ccactaaaca
    11161 ctctagcctc tgcacctggg tcaactgata cggtgctttg gccgtgacaa tgctcgtaaa
    11221 gattgccacc agggcgaagg aagaaatgac ttcgcctccc gtatctggaa aggtgtacat
    11281 ggcttcacaa ctgactgatg catttgcgcg taagttttac tacttgcgcc tgtcgattac
    11341 cgatgtgtgt aactttcgtt gcacctactg cctgccggat ggctacaaac cgagcggcgt
    11401 caccaataaa ggctttctta ccgtcgatga aattcgccgg gttacgcgcg ccttcgccag
    11461 tctgggcacc gaaaaagtgc gcctgacagg aggagagccg tctttacgcc gcgactttac
    11521 cgatatcatc gccgctgtgc gggaaaacga cgctatccgc cagattgcgg tcaccaccaa
    11581 tggttaccgt ctggaacgcg atgtggcgaa ctggcgcgat gcgggactta ctggcattaa
    11641 cgtcagtgtc gacagtttgg acgcccgcca gtttcacgct attaccgggc aggataaatt
    11701 caaccaggtc atggcaggga ttgatgctgc atttgaggcc ggttttgaga aggtcaaagt
    11761 caataccgtg ctgatgcgtg atgttaatca tcaccagctc gacacctttc tgaactggat
    11821 ccagcatcgc cctatccagc tgcgtttcat cgaactgatg gaaacgggcg agggcagcga
    11881 gctcttccgt aagcatcaca tctctggtca ggttctgcgt gacgagctac tgcgtcgcgg
    11941 ctggatccac caattacgtc aacgcagcga cggtcccgcg caagtctttt gccatccaga
    12001 ttacgccgga gagattggcc ttatcatgcc gtatgaaaaa gacttctgcg ccacttgcaa
    12061 ccgcctgcgc gtttcctcca ttggtaaact ccatctctgc ctgtttggtg aaggcggcgt
    12121 taacctgcgc gatctgctgg aaggcgatac ccagcaacag gcgctggaag cgcgtatttc
    12181 agcggcgctg cgggagaaga aacagaccca tttcctgcat caaaacaaca ccggtattac
    12241 gcaaaactta tcgtacattg gcggctaaaa cgtcaaaagg agagatcaga tgagtcaggt
    12301 aagcactgaa tttatcccga cccgtattgc tattcttacg gtttctaatc gtcgcggtga
    12361 agaagacgat acctccggtc actatctgcg cgattcggcg caagaagcgg gccatcacgt
    12421 tgtcgatgaa gccattgtga aagaaaaccg ctacgctatt cgcgctcagg tatctgcgtg
    12481 gatcgccagc gacgatgtac aagtggtatt gattacgggt ggtactggcc tgacggaagg
    12541 tgatcaggct cccgaagcgc tgctgccgtt gttcgaccgt gaggttgaag gttttggtga
    12601 agtgttccgt atgttgtcgt ttgaagagat tggcacttcc acgttgcaat ctcgtgcggt
    12661 agcgggcgtc gctaacaaaa cgctgatttt cgccatgccg ggttcgacca aagcgtgccg
    12721 taccgcatgg gaaaatatca tcgcgccgca gctggatgcc cgtacgcgtc cgtgtaattt
    12781 ccatccacat ttgaagaaat aagtatgtcg caactgaccc atatcaacgc cgctggcgaa
    12841 gcgcacatgg tggatgtctc cgccaaagcg gaaaccgtgc gtgaagcgcg ggcggaagcc
    12901 tttgtcacca tgcgcagcga gacgctggcg atgattattg atggtcgcca ccacaaaggc
    12961 gacgtatttg ccactgcgcg tattgccggt attcaggcgg caaaacgcac ttgggatctg
    13021 atcccgctgt gtcacccgct gatgctcagc aaagttgaag tcaatttaca ggccgagccg
    13081 gagcacaatc gggtgcgtat agaaacctta tgccgcctga ccgggaaaac cggtgtcgaa
    13141 atggaagcgt taaccgcggc ctccgtggcg gcgctgacca tttatgacat gtgcaaagcg
    13201 gtgcaaaaag atatggtgat tggtccggta cgtttgctgg cgaagagcgg cggcaagtcg
    13261 ggtgacttta aggtggaagc ggatgattaa agttcttttt ttcgcccagg tgcgcgagtt
    13321 ggtgggaaca gatgcaaccg aagtggctgc ggattaccca accgttgaag cgttacgcca
    13381 gcacctggct gcgcagagcg atcgctgggc gctggcgctg gaagatggca aattgctggc
    13441 tgccgtcaac cagacgctgg tgagttttga ccatccgctg actgacggcg acgaagtagc
    13501 tttcttcccg ccggtaaccg gaggttaaga tggcagaaac caaaattgtt gtcggcccgc
    13561 agccgttcag cgtaggagaa gagtacccgt ggctggcgga gcgtgacgaa gacggtgcgg
    13621 tagtcacctt caccggaaag gtgcgcaacc ataacctggg cgacagcgtc aaagcattaa
    13681 ccctcgaaca ctatccgggg atgactgaaa aagcactggc agaaattgtt gatgaagcgc
    13741 gtaaccgctg gccgctgggg cgcgtcacgg tgattcaccg catcggggaa ttatggccgg
    13801 gcgatgaaat cgtttttgtc ggtgtcacca gtgcgcatcg cagcagtgcg tttgaagccg
    13861 ggcagtttat tatggattat ctcaaaaccc gcgcaccgtt ctggaagcgc gaagccacgc
    13921 cggaaggcga ccgctgggtt gaagcccggg agagcgatct gcaggcggca aaacgctggt
    13981 agttttttgt tagccggata aggcaccgct gcgtccggca atttcaccgc tcagtacatg
    14041 cgtttacgct gcgtcgatgc acagcctcat cattttgcag tatccttaag atattcctta
    14101 tatcttcagg agatcgtcat ggacagattc ccacgttctg attcaatcgt acaaccccgg
    14161 gctggcttgc aaacttatat ggcgcaagtc tatggctgga tgaccgttgg cttgttgctg
    14221 accgcatttg ttgcctggta tgcggctaat tccgcggccg tgatggagct gttgttcact
    14281 aaccgtgtct ttttaatcgg tctgatcatc gcgcaattag cattggttat tgtgttatca
    14341 gcgatgattc aaaagctgag cgcaggtgta acgacgatgc tctttatgct ttattcggcg
    14401 ctgacgggtc ttacgctttc cagtatattc attgtctata ccgctgcttc tatcgccagt
    14461 actttcgtcg ttactgccgg gatgttcggc gcaatgagcc tgtacggtta caccacgaag
    14521 cgcgatttaa gtggctttgg caatatgctg tttatggcgt taatcggcat tgtgctggca
    14581 tcgctggtca acttctggct gaaaagcgaa gcattgatgt gggcagttac ctacatcggc
    14641 gtgattgtct ttgtcggatt gacggcgtat gacactcaga aattgaaaaa tatgggtgag
    14701 cagattgata cccgcgacac gtcgaacctg cgcaaatatt ccattcttgg tgcgttaacc
    14761 ttgtatctgg acttcatcaa cctgttcctg atgttgttac ggatcttcgg caaccgccgt
    14821 taattctttt tcaccatcac tatcccggcc atgtgtcggg atttttttct tactaaaaac
    14881 gacacttaac aagtaccagg tataaaatat gccttatgag cgagcggaat cgttttccgt
    14941 atccataaat acaaaagtat tttgtatgcg tggttttaaa tttattgatg gtgaatatta
    15001 atgttttttc taaggacaac taaaagaatg gaatcatact cgcaaaacag caataaatta
    15061 gattttcagc acgaggccag gatattaaac ggtatatggc tcattaccgc tttaggtttg
    15121 gtggcaaccg caggactagc ctggggagct aagtatctcg aaattacggc aaccaaatat
    15181 gattcaccac caatgtatgt cgccataggg ttattattac tttgtatgta tggcttaagt
    15241 aaggatatca acaagataaa tgccgccatc gcgggcgtaa tatatctgtt tttactctct
    15301 ttggtggcga ttgtcgttgc aagtttagtt cctgtatatg ccattatcat cgtgttcagc
    15361 actgcgggcg cgatgttttt aatcagtatg ctggccggtt tattatttaa tgttgatcct
    15421 ggttctcacc gttttatcat tatgatgacg ttgacagggt tggccctggt aatcatcgtg
    15481 aatgcggcat taatgagtga acggcccatt tgggtaataa gttgcttaat gattgtgtta
    15541 tggccaggca ttatctcgca tggacgaaat aagctccttg aacaggcagg aaaatgccat
    15601 agtgaagagt tgtggagtcc ggttcgttgc gcttttacag gtgcattaac actctattac
    15661 tattttatcg gcttctttgg gatacttgcc gcgatagcta taacgcttgt ctggcaaagg
    15721 catacgcgtt tttttcatta gtcgtaaatg cagatactaa agaacttaac taaacttcac
    15781 atcgccgctt catttttcgc ccgtagcttc ttcgcttggc tttccagtag caggtaacag
    15841 atcagcgcca gcagcagcgg gataaagtaa tacaggacgc gataagcgag cagggcggcg
    15901 ataatgctcc ctttcgaggt atgctcccca gccagtagtg cgataaacac cgcttccagc
    15961 acaccgatcc ccgccggaat atgcacgatg acgccagcaa tactactaac cagtaacacg
    16021 cccagtacaa agaaatagtt cacgctttga ccaagtaaca gccagataat cgcccccatt
    16081 accatccagt taacgctgga aatcagcatc tgagcgaggg cgaatttcca tgaaggcagc
    16141 accagctttt gccctttgat ggtcatatgg cggtgcttcg cgaaagcgca aaaccacaaa
    16201 taaaccgcga taatcatcag taagccaatg ccgagaatac gcagcgtagt ttgatcgaca
    16261 taccagtaat ccggcaactc caccacgcct gcggtaaaga taatccctgc cagtaaaata
    16321 tagcccagcc agttggtggt aatactgagc gaggaaatcc gcgtaatagt gctgcccggt
    16381 aaccctagtc gggagtacaa acgatagcgc ataccaatgc cgccgaccca ggtactgagc
    16441 gtcaggttga aggcgtagca gataaacgac accagcatca cctggcgctt cgccagtttg
    16501 tgaccgcagt aaaagcgggc gagcaggtca tagcagccgt aaatcagata gctgacgacc
    16561 accagcccga ccgcactaag cagcgcaacg cgattgtagt cgcggatgac cttccagacc
    16621 tcttcccagt ccactttttt ggcgtagacc accagcaaca caatcaccgc gataaaaaac
    16681 agccaggtga ggatcttctt tgctaagcgc cagcgcgggt gtgatttact catcaggggt
    16741 ttacccccgt gttttcagtt tctacccgat cctgcgtttc cattgtcggt tgtgcggggg
    16801 gatcaacctg cgtcagacgt ggtgtgtgtg ccggaagcca gccaaccagc gccgggaagt
    16861 ggcgtaaaaa gtggaacgcc agcacgcttt tggtcaggtt ccaccaggtg cgtttgggca
    16921 gcatggtttc atccacctgc tgacaatctg cggcaataat gccgttcaga ttatcgcgca
    16981 gcgtctggtt aaaatgacga tcgtggatga tgacatttgc ttcgagattc agtgacaaac
    17041 tgagcggatc gagattactg gaccctactg tcgcccagtg atcgaccatc aatgccactt
    17101 tgccgtggag cgggcggcgg cggtactcaa aaacctgaat gccgccttta accagatagt
    17161 tatacagcaa gcgcgcaccg actctgacaa tcggcatatc cggttcgccc tgaatgatca
    17221 gtttgatccg caccccgcgc cgtgccgctt tacgcaaggc gtgtaaaaat cgatagccgg
    17281 ggaagaagta ggcgttggcg ataatcactt cccgctgcgc ctgagtgagc attttcagat
    17341 aatggcgttc aatatcatcg cgatgttctt cgttatcgcg ccagaccagc aatacctgcg
    17401 cttctcccgg ctggcggttc tcttccgctt tgtgatgacg tcgccaccag cgtcgtgccg
    17461 cgctctgtcc aggcaggttt tccagctcaa actggagaat atcttcgaca atcggccctt
    17521 caaggcgtac cgcgtaatcc tgtttagcct ctggaccgta gctggacata tgctcggcgg
    17581 agtaattcag cccgccaata aaggcgatac gcgcgtcgat caccacaatt ttgcgatgca
    17641 tccggcgaaa cacattggtg cgcataccaa aaaggcgagg gcggggatcg tagtagcgga
    17701 acactacgcc agctgccgtc agttcattga caaactcatc gctgagatcc ggcgaaccgt
    17761 agccatccag caagacttcc gctttaaccc cgcgttgcgc tgctgccagt agtgccgcat
    17821 gcagctgttt gccgacgtta tcctcaaacc agataaacgt ttcaagaatg atgcgttctt
    17881 gtgcctcgcc aatcgcctta aacaccgcgg gataatattg ctcgccgttt tccagcaact
    17941 ggatcttatt gccttcgcgc cagctacatt tcataaatga atctccgcac ttaaaggggc
    18001 atgatcagaa aggtgtcgcc atgtccgcag cggcaacgcg gttggcgcgc tggcgctggc
    18061 atttttgacg tagatcctgt ccagtcgtag tagaggaaat tgcaccggaa acgtgcgcgc
    18121 cgggcgtccg tgggcgcggg taaaaatctc atccagtccg gcctgcactt ttaacggatg
    18181 attagctttt tgccgccagt cattgaaatc acccgccacc aataccggtt cgccgtccgg
    18241 tagctcattc acccattcgg cgagcatcgc aagctgcgcc tgacggtgcg cctcacgcag
    18301 gcccagatgt acgcacatca catgaatcgc ttttccggtc atcggcggca caatgcggca
    18361 gtagagcacg ccgcgctttt ccgcaccatc gaccgaaaca tcgcgattct cataatgttc
    18421 aatgggataa cgcgacagta cggcgttgcc gtgatgccct tccgggtata cggcattgcg
    18481 accgtaggca aaatcgctcc acataatgtc ggcgagaaac tcgtagtgcg aggtatcggg
    18541 ccagttttcc acatgcagcg gatgaacttc gtgcgcgccc atcacttcct gcaggcaaac
    18601 aatatcggcg ctgacggtac gcacggcatc gcgaagttcc ggcaaaatga agcgtcggtt
    18661 aaacgcggta aagcctttgt gaatgttgat ggtgagcacc ttgaacgaaa attgttgtgt
    18721 ttgatcgggc ataattttcc tgtctttgcc tcttatctca ttgaaatagt gtagtcggcg
    18781 tcacaaaaag gtgcggtctt acggaatttt ccgtaaagtt cggtactctg agtaagtaga
    18841 gataaattct tcaggagaga agccatgaag tggcaacaac gtgttcgtgt cgcaacgggt
    18901 ctaagttgct ggcagattat gttgcattta ctggtagtgg cgctgctggt ggtgggctgg
    18961 atgagtaaga ctctggttca cgtcggcgtg ggattatgcg cactgtattg tgtcacggta
    19021 gtgatgatgc tggtgtttca gcgccacccc gagcaacgct ggcgtgaggt ggcagacgtg
    19081 ctggaagagc tgaccacgac ctggtatttt ggcgcagcgc tgattgtgct gtggctgttg
    19141 tcccgcgtac tggaaaacaa ctttttgctg gcaattgcag ggctggcgat ccttgctggc
    19201 ccggcggtag tgtctttgct ggcgaaagat aagaagttac atcaccttac gtctaaacat
    19261 cgcgtacgcc gctgaccctg tcgtggccgt tatcaccagt agcggccaca aactattcca
    19321 cacaatatcc agactcgcat ccttcaaata aatctgcttg gtaatgtccg taaagtggcg
    19381 aatagggtta atccacgtca gattttgcag ccacactggc atgttttcca ccggcgaaac
    19441 gtaaccggaa aggagaatgg cgggcatcat aaaaacaaac acgccgataa acgcctgctg
    19501 ttgtgtcgag cagagtgatg aaatcaacag accgaatccc accagcgata aaccgtaaat
    19561 caccatcgta aagtagaaca gcgctagcga tccggcgaag gggatttgat acgcccagat
    19621 accaatcgcc agcacaatgg tggcctggaa ggtggcgaca attaacgccg gtacggcttt
    19681 gccgatgaag atctgccagg tggtgagcgg cgaaaccagt agctgatcga gcgtaccttg
    19741 ttcacgttcg cgggcgacgg aaagtgaagt gacgatcatt acgccgatag tggtgatcat
    19801 ggcgatcagc gacggcacca caaaccattt gtagtcgaga ttcgggttat accagttgcg
    19861 taccaccagc tcgctgttgt tgggtttcgg ttttccttcc agcagctcct gctgataatt
    19921 tttgacgatc tgttgcaggt agttggcggc aatttgcgca ctgttggagt tacgcccgtc
    19981 gaggatcaac tgcaacggcg cggtctggaa ggtatccagt ttgcgcgaga agtcagccgg
    20041 gaaacgcacc agtagtaacg ccttttgtgt gtcgatggtc gggcggatct cctgtgggct
    20101 tttcagcagc agcacatgtg taaaggcgct ggcgcgggca aaacgttgag taagttccac
    20161 cgaatgctcg ccgttatctt catcgtagat ggcgatggtg gcgttagtca cttccagcgt
    20221 ggcggcgaac gggaacagga tcacctgaat tagcacgggt aaaatcagaa tcgcgcgggt
    20281 ttgtggttcg cgcagcaacg actgcaactc tttgcggatt aacgtccata agcgatgaaa
    20341 catgctcttc tccctaatcc agccgacgtt tggttttcag ccacgtcagg ccgataaaca
    20401 tcaccgccga agcgatcaaa aacagcacgt ttaccaccag caccactgga atattcccgg
    20461 cgaggaacag gctttgcagg gtgctgacga aataacgagc gggaataatg tacgtcaccg
    20521 cgcggatcac cgcgggcata ctgtcgatct gaaaaataaa gccggaaagc ataatcgacg
    20581 gcagaaaagc ggcgttcagg gcgacctgag cggcattgaa ctggttgcgg gtaatcgtgg
    20641 aaatcagcag ccccattccc agggtactga gtaaaaacag gctggagata aaaaacagaa
    20701 tcagcagcga accgcgatac ggcacgccga gaataaacac tgacaccagc atacacagca
    20761 acatcgccag catcccgaga aagtaataag ggatcagctt acacagcagc agttccgtgc
    20821 gggtaatctc cgtagagagc agagcctcca tggtgccgcg ttcccattct cgcgccacca
    20881 ccagcgaggt gagaatcgcg ccgatgaccg tcatgataat ggtcaccgca cccgggataa
    20941 taaagtgctg gcttatcgcc gccgggttaa accagtagcg ggtttgtaca tcaataagcg
    21001 gttcaaaagt ctgcccgttg tcctccgctc gctgcatttg ccagatctgc cagatccctt
    21061 cgacataccc ctgcacaaag ttagcggtat tcggctcact gccgtcggtg atcacctgaa
    21121 tcggtgcggt ggcgttggcg cgctccatct gttcggcgaa atccacggga ataaccacca
    21181 gaccgcgaat tttccccgac tgcattttgg cgatcagttc ctgacggtta tcgctgatgg
    21241 tggcgtcgat gtagggcgaa ccggtcatgg tgtgggtgaa atccagcgcc gcttcgctac
    21301 gctgttccag caaaatcccg acccgcagct tgctggagtc gaggttaatg ccgtaaccaa
    21361 aaataaacag cagtagcagt gggatcacta ccgcaatcag ccagctactc ggatcgcgaa
    21421 cgatctgccg cgtctctttg acgcacagcg cccgcacgcg atgccaggaa aggatcgggt
    21481 tactcattgc tatgctcctt atcccagtcg tggatcaact gaataaaggc ttgctccatg
    21541 gtgggatcgg gttgctcatc gttagctgac tgtgctttca aatcgtccgg cgtaccgctg
    21601 gcgattaatt tcccacggta caccaggccg atgcggtcgc aatattccgc ttcatccata
    21661 aagtgggtgg tgaccatcac cgtgacgcct ttctctacca tgctgttgat gtgcagccaa
    21721 aattcacggc gggtgagggg gtcaacgccg gaagtcggtt cgtcgagaaa gagaatatcc
    21781 ggttcatgca tcagcgaaca ggccagcgcc agccgctgtt taaaacctaa tggcagttca
    21841 tcggtggcgt gtgaggcaat acttttcagg ccaaatgcct cgctcatacg ggagattttt
    21901 tcgttctgcg cccgaccgcg taagccatac acaccagaga aaaagcgtaa attctgttcg
    21961 accgtcaggt tgccgtagag cgaaaatttt tgcgccatat agccgagatg ctggcgcgct
    22021 ttaccggaac tctctttcag atccatcccc agcaccagcg cctggccgga agtcggcacc
    22081 agcaaaccgc acatcatctt aaaggtggtc gatttacccg cgccgtttgg ccccagcaaa
    22141 ccaaaaatct ccccacgttt aacggcaaag ttgacgtgat cggtggcggc aaaatcacca
    22201 aatttcttgg tcagttcttt cgcttcgatc accgtctcgc cgggtgtgcc ttctaccgta
    22261 tgcaatattg cacccagcgg cgattctgag gttccggcac cgcccagcaa atcaataaac
    22321 gcatcttcaa aacgcggcgt agtttcgttg atgttgattt ccggcatccc gtcggcatgg
    22381 cgaatatcgt ctggtgtggc ctcttttttg aggatcagac gtaccgattt cccctgaatc
    22441 atgccatcgc tgacctgcgg cagtttcaag gcgcgttgca acagtttgcg gttgccctcg
    22501 tgtggactgg tcatcagaaa gctgcgtccg gccatggttt gtgtcagggc ttttggttct
    22561 ccctgataca gcaactcgcc ttcgttcatc agtaacacgt cacggcactg ctcggcttcg
    22621 tcgagatacg aggtactcca gaggattaac atcccttcgc ccgccagctc atgcaccatc
    22681 tgccacagtt cgcgccgtga gatagggtca acgccgacgc cgggttcatc gagcagcaac
    22741 actttcggtt cgcccaccag ggtacaggcc aggccgagtt tttgtttcat cccaccggag
    22801 agcttgcccg ccaggcgtcc ggtaaacggc ccaagagacg tgaactccag caggcgagca
    22861 aaagtttgct tacgtgcctc gccggtgacg ctgcgcaaat ccgcgtacag attgaggttc
    22921 tccatcaccg tgagatcttc atacagacca aatttctgcg gcatatagcc gagcacggcg
    22981 tgcagcgcgc cgtcgttttt gatcggatca aagccaatca ccgtggcact gccgctgtcg
    23041 ggtttcagta atcccgccaa catccgcatc agtgtggttt tacctgcacc gtccggcccc
    23101 accaaccccg tcacataacc ggcgtgaatg gtacaatcga gcggcgcgac ggcgggcttg
    23161 tccatgcccg gaaagcgttt ttccaggccg ttcagcgtga taacggcatc attcatgtcc
    23221 tgcctcgtca ccgaattgta ccgtcactgg cattccctgg cgtaacgcat catcggcgtc
    23281 ggtcaccaca atacgcaggc gatagacgag gtcggtacgc agatccggtg tttcgacggt
    23341 tttcggggta aattcagcag tcggcgaaac gaaaccaatc tgcccgtgat acggcttgtc
    23401 cgggcgacca tcggtataaa gcagcacttt gcgccccggc tgggcctggt caagattccg
    23461 ttcatcaaca taagcgcgca cccacaccgg acgcgttagt gaaacggtaa acaccgtgcc
    23521 accttcattg agaaccgtgc ctggctctac cgcgcgcgtt aacagcgtgc catcagacgg
    23581 tgctatcaac gttgagtcct gtaaattcag ctccgcctgc gccagttgcg cctgcgcctg
    23641 ttcgaggctg gctttcgcct gagcgatgtc ctgttcacgg ttaccggaac ggtactgacg
    23701 caatttatcc tgtgctgatt tcagcgttgc ctgtgcctgg tcgcgcgagg agcgggcatt
    23761 ttccaggtca tttgccgaaa tggtgcggct tttccacagt ccttgctggc ggttatagaa
    23821 gttctgcgca tagtcatagg cggcttgcgc ctgtttcacc gctgcggcgg cctgagcgat
    23881 ttcttcatcg cgatacccgg caagcatcag attatactgc gcctgtgcca ccgagacgcc
    23941 cgctttcgcc tgcatcaggg caatctcata cggcttgtga tccagttcgc ccagcacctg
    24001 gcccgctttg atagcatcac cttcgtccac cgccagcgat tcaacgcgcc ccccaacacg
    24061 gaaactaaga ttcaccgtac gaatatccac gttgccatac agcgtcaggc cgttatcctg
    24121 gcggctttga taccaccagt agcctccggc aaccacggcg gcaagtacca ctaccgccag
    24181 tccgatcacg acaggttttt tcatcactac aaactccttt gcgataatcc ttgcaaaatc
    24241 aggtcgatat gacaggtcac cgtctggttg atcagttcgg tcttttcttc atcgaacgcg
    24301 gtccagccgg tacgtaacag aattgtttct ttgccaagac ggaacgccag aatctcacca
    24361 atcaacgcat gggtatgaag gatcatgcgg gtgtcattag cgtcgcagcc ggtccaggcg
    24421 gcaatcaggc gtgtcaggtg gctgtgtagc ggactaatca cctgttcgtg caccaggtgg
    24481 taggctgccg tgggagagag ctgctcacgg gagataaact tgctgaggtt gacggtgtca
    24541 tcctgggtga gcagcttaat catgttcctg caggcgcgaa ggatcagttc acggatggca
    24601 gcccgatcag gctgtggttg ggcgaacaag cgttcggctt cctcggcatg cggacggaac
    24661 tgctcgccaa taaaatcggc aatccactgg gcgcaggcga ggtacaaatc ttctttcgaa
    24721 ccgaagtagt aggtgatggc agcgatattc tgcccggcct gggcggctat ctcgcgggtg
    24781 gtggcgttca ttccatattc accaaactgc gccagtgcgg cagcaatcag ctgttttttc
    24841 gcctgttcac ccttgattgt catggcagga ttattcatcg cacagtccat tcttaatcaa
    24901 atgattgatt aagattatga ctccatatgg gagttgtcca gtatggctaa gaattttagc
    24961 tacgccagtc acagggataa tttatgcgct gcgtcacaaa aactgctaca ctccgctccc
    25021 tcatgacatt gtggtttttg tcattttcct tttcagtatc tccctgaaaa ctacaccggt
    25081 aacggtcggg gcggttcgga gtagttatgt ctttcgattc tttgggttta agccctgata
    25141 tcctgcgcgc cgttgccgag cagggttacc gtgaacccac ccctattcag cagcaggcga
    25201 tccctgcggt gctggaaggc cgcgacctga tggctagcgc ccagaccggc accggcaaaa
    25261 cagcgggctt tacgctgccg ctgttgcaac acctgatcac tcgccagccg cacgccaaag
    25321 ggcgtcgtcc ggtacgtgcg ctcattctta ccccgacccg tgaactggcg gcacagattg
    25381 gcgaaaacgt ccgtgattac agcaaatacc tgaacattcg ttcgctggtg gtgtttggcg
    25441 gtgtcagcat taacccacaa atgatgaagt tgcgcggcgg cgttgatgtg ctggtggcaa
    25501 cgccgggacg tttgctggac ctggaacatc aaaatgcagt gaagctggat caggttgaaa
    25561 tcctcgtcct cgatgaagct gaccgcatgc tcgacatggg ctttatccac gatatccgtc
    25621 gcgtgttaac aaaactgcca gcgaaacgcc agaacctgct gttctctgcg accttctctg
    25681 acgatattaa agccctggcg gaaaaactgt tgcacaaccc gctggaaatc gaagtggcac
    25741 gccgcaatac cgcgtctgat caggtgaccc agcacgttca ctttgtcgat aagaaacgca
    25801 aacgcgaatt gctgtcgcac atgattggta aagggaactg gcagcaggtg ctggtgttta
    25861 cccgtaccaa acacggcgct aaccatctgg ctgagcagct caataaagat ggcatccgta
    25921 gtgtggcgat ccacgggaat aaatcgcaag gtgcgcgtac ccgtgcgctg gctgatttta
    25981 aatcgggtga cattcgcgta ctggtggcga ccgatatcgc tgcgcgcggt ctggatatcg
    26041 aagagttgcc gcacgtcgtc aaccatgaac tgccaaacgt accggaagat tatgtccacc
    26101 gtatcgggcg taccggtcgt gcggctgcta ccggtgaagc gttgtcgctg gtgtgtgttg
    26161 atgaacacaa actgctgcgt gatatcgaaa aactgctgaa aaaagagatc ccgcgcattg
    26221 cgattccggg ctatgagccg gacccgtcaa tcaaagccga accgatccag aacggtcgcc
    26281 agcaacgtgg cggcatcagg ctgatgaaca aacgcaaaac tgcctgatgc gctacgctta
    26341 tcaggcctac gtgaactctg caatatattg aatttgcatg cttttgtagg ccggataagg
    26401 cgtttacgcc gcatccggca tttcacaaca agcacttgtc agcaatttga gaacacggga
    26461 aaaaatttat ctgttttacc gcccataagc caccgagacg ttacttctct atagccaatt
    26521 gttcgcgcaa ctccattaaa aggtagccca gtcgattctt gcccttacca ttaccaccgt
    26581 ctccccagta agcatcgttt tccgtatgct caaccagttt tgcgggcgcg gttgccagca
    26641 agagcacacg cagttctgca tgctgttcga atttagcgcg aagtgcttta cgcatcacct
    26701 gttctttaac cgactcccag tttttacgaa gtggcttgga acgattacgc cccatgcgag
    26761 cggcgaccat gggggaagaa acccgacgga tctcttcgcg gtatttttca tcaaggaatt
    26821 tttgtgcctg aaaatagtgt tcggaggtgg gccaggtatt cccgtcaacc ttgatgggcc
    26881 tggcggcaaa gttagaaaaa tcaccgtagt catcgctggt gctgtagaaa tttatgatgg
    26941 tgtcttgcat gacgtgttgg atcctttgtg ctcgaacggg cattaaaccg cattatgttg
    27001 gtggttattg cgagccgctt tccagaaaca gaaaaaccat tacccctgaa aaccgaaaaa
    27061 tgccacaata ttggctgttt atacagtatt tcaggttttc tcatggcatt aaccgccgcg
    27121 cttaaagcgc aaattgccgc ctggtataag gcgcttcagg aacagatccc cgactttatt
    27181 ccccgtgcgc cgcagcggca gatgattgcg gacgtcgcca aaacgctggc cggagaagaa
    27241 gggcggcatc tggcgattga agcccccacc ggcgttggga aaacgctctc ctatttgatc
    27301 cccggcatcg ccattgcccg cgaagagcaa aaaacgctgg tggtgagtac cgccaacgtg
    27361 gcgttgcagg atcagattta cagcaaagat ttaccgctgc tgaaaaagat cattcccgat
    27421 cttaaattca ctgccgcttt cgggcgtggg cgctacgttt gtccacgtaa tctgacggcg
    27481 ctcgccagca ctgaacccac gcaacaggat ctgctggcgt ttcttgacga cgaactgacg
    27541 ccaaataatc aggaagagca aaaacgttgt gcgaagctga agggcgatct cgacacttat
    27601 aaatgggatg gtctgcgcga tcatacggat atcgctatag atgatgatct ctggcgtcgt
    27661 ttgagtaccg acaaagccag ctgcctcaac cgtaactgtt actactatcg tgaatgcccg
    27721 ttttttgtta ctcgtcggga gattcaggaa gcggaagtgg tggtggcaaa ccatgcgctg
    27781 gtgatggcgg cgatggaaag cgaagccgta ctgcctgacc cgaaaaatct gctgctggtg
    27841 ctggacgaag gtcatcacct gccagatgtg gcccgggatg cgctggagat gagcgctgaa
    27901 atcaccgcgc cctggtatcg actacagctg gacttgttca cgaaactggt cgctacctgc
    27961 atggagcagt ttcgcccgaa gaccatccca ccgctggcga tccctgaacg tttgaatgcg
    28021 cattgtgaag agttgtatga gcttatcgcc tcgttaaaca acattctcaa tctctacatg
    28081 cctgccgggc aggaggcaga gcaccgtttt gcgatgggcg aactgcctga tgaattgctg
    28141 gagatctgcc agcggctggc aaaactcacc gagatgctgc gtggtctggc ggagttattt
    28201 cttaacgatt taagtgagaa aaccggcagc cacgatattg tgcgtctgca tcggttgatt
    28261 ttacagatga accgcgcgct ggggatgttt gaggtgcaaa gcaaactctg gcggctggcg
    28321 tctctggcgc aatcttccgg tgcgccggtg accaaatggg cgacgcggga agagcgcgaa
    28381 gggcagctac acctctggtt tcactgcgtg ggaatacgtg tcagcgatca gctggaaagg
    28441 ctgctgtggc gcagtattcc gcacattatt gtcacctccg caaccttgcg ttcgctgaat
    28501 agtttttcgc gtttgcagga gatgagtggt ctgaaagaga aagcgggcga ccgttttgtg
    28561 gcgctggatt ccccctttaa ccactgcgaa cagggcaaaa ttgttattcc ccggatgcgc
    28621 tttgagcctt ccatcgacaa cgaagagcaa cacattgccg aaatggcggc ctttttccgt
    28681 gaacaggtgg agagcaaaaa atatctcggt atgttggtac tgtttgccag cggacgggcg
    28741 atgcagcgct ttctcgacta tgtgacggat ttacgcctga tgttgctggt gcagggcgat
    28801 cagccgcgtt atcgcttagt tgaactgcac cgcaaacgcg ttgccaacgg tgagcgtagc
    28861 gtgctggtgg gcttacagtc atttgccgaa gggcttgatt tgaaaggtga tctgctcagc
    28921 caggtgcata tccacaaaat cgcttttccg cccatcgaca gcccggtggt gatcaccgaa
    28981 ggggaatggc tgaaaagcct caaccgctat ccgtttgagg tgcaaagcct gccgagcgcc
    29041 tcgtttaacc tgattcagca ggttgggcga ctgattcgaa gccacggttg ctggggcgaa
    29101 gtggttatct acgacaaacg cttgctgaca aaaaattatg gcaagcgact actggatgca
    29161 ttaccggtat ttccgataga gcaaccggaa gtccctgaag gtatagttaa aaagaaagaa
    29221 aaaacgaaat ccccacgccg tcggcggcgt taatgatgtg agtcaggtaa ggagtcgtaa
    29281 atggactatc gcaaaatcat taaagagatc gggcgcggga aaaaccacgc gcgcgattta
    29341 gaccgggata ctgcgcgcgg tctgtatgct catatgctca atggtgaagt ccctgacctt
    29401 gagttgggcg gcgtattgat tgcgctgcgt atcaaagggg aaggggaagc agagatgctc
    29461 ggcttttacg aagccatgca aaatcacacc atcaagctga cgccgcctgc aggcaagccg
    29521 atgccgattg tcatccccag ttacaacggc gcgcgtaaac aggctaacct gacgccatta
    29581 ttggcgattc tgctgcataa actcggtttt cctgtggtgg ttcacggagt tagcgaagat
    29641 ccaactcgcg tgctgacaga aaccattttc gaactgatgg gtattacacc aacgttgcac
    29701 ggcgggcagg cgcaggcgaa gctcgacgaa catcaaccgg tgtttatgcc tgtcggcgcg
    29761 ttttgcccgc cgctggaaaa acaactggcg atgcgctggc ggatgggcgt gcgtaacagt
    29821 gcacataccc tggcgaaact ggcgacgcca tttgccgaag gtgaggcact gtgtctttcc
    29881 agcgtttcgc atccggaata cattggacgc gtcgcgaagt tctttagcga tatcggcggg
    29941 tgggcgttac taatgcatgg tactgaaggc gaagtgtatg ctaatccgca gcgctgtccg
    30001 cagatcaatc tcattgaccg tgaagggatg cgggtgctgt atgaaaaaca ggaccctgct
    30061 ggtagcgagt tactgccaca agcaaaagat ccggaaacca cggcgcagtg gactgagcgt
    30121 tgcctggctg gcagcgaagc gattcccgaa tcgctgaaaa tccagatggc ttgctgcctg
    30181 gtggctacgg gtgaagcggc aactatcagc gatggcctgg cgcgcgttaa tcaggcattt
    30241 taatttttat ctcagccgga tgagccacgt catccggctt ttaccccacc gcaatatgaa
    30301 attcctgcat ctttattgac cttcccgcgc ccggcgtgca gcataaaaaa acaacaaaca
    30361 cataacataa acaggagtta accatggaaa gtggtcatcg ctttgatgct cagacgctgc
    30421 acagttttat tcaggctgta tttcgtcaga tgggtagcga ggaacaagaa gcgaaattag
    30481 ttgccgatca tttaatcgcg gcaaacctgg cagggcatga ttctcatggt attggcatga
    30541 tcccaagcta tgtgcgttcc tggagtcagg ggcatctgca aattaaccat catgccaaaa
    30601 tcgttaaaga ggcgggggcg gcagtcacgc tcgatggcga tcgcgcattc ggtcaggtcg
    30661 cggcacacga agcgatggcg ctggggattg agaaagcgca tcagcacggt attgctgccg
    30721 tggcgctaca taactcgcat catatcggcc gtatcggtta ctgggcggag cagtgttcag
    30781 cggcggggtt tgtctctatc cactttgtta gcgtggtcgg tattccaatg gtcgcgccgt
    30841 tccacggtcg cgacagccgc tttggcacca atcccttctg tgtggttttc cctcgtaaag
    30901 ataatttccc gctgttgctt gattacgcca ccagcgccat tgcatttggc aaaacccgcg
    30961 tcgcctggca taaaggcgtc cccgtgccgc caggttgcct gattgacgtt aacggcgtgc
    31021 cgacgaccaa tccggcggta atgcaggagt cgccgttggg gtcgctgttg acctttgccg
    31081 aacataaagg ctacgccctt gcagcgatgt gtgaaattct tggcggggcg ctttctggcg
    31141 gtaaaacgac gcatcaggaa acgttgcaaa ccagccccga tgccattctt aactgcatga
    31201 ccactatcat catcaacccg gaactgttcg gcgcgccgga ttgtaacgcg cagaccgaag
    31261 cctttgccga gtgggtgaaa gcctcgccgc atgatgacga taagccgatt ttgctaccgg
    31321 gcgagtggga agtgaacacg cgtcgcgaac ggcaggagca ggggattcca ttggatgcgg
    31381 gaagctggca ggccatttgt gatgcggcgc ggcagattgg tatgtcggaa gagacgttgc
    31441 aggctttctg tcagcagtta gccagctaaa aaaaagcccg tccagtggcg gacgggcaaa
    31501 caagggtaac ataggatcaa tgagggttag agcatatgcg tctgtcggca aacagacagg
    31561 gaaatacttg tgctggacgt agcgtaaacg cctgatccgt ccaacggttc ggattttgta
    31621 ggcctgataa gacgcggtag cgtcgcatca ggcatctgtt gccggatgcg gcataaacgc
    31681 cttatccgtc ctacgaatta cttataaatt accgcagtac cgcttaattt attgttgttg
    31741 gtggcggaag tgatgctgta tccactggca ccggctgccg ccgctttctc agccagtttc
    31801 gcttccaggg catcgagggt ggatgcgcca tcggcagaaa ccacgccgat tttattcatg
    31861 ttctgtgcct gggatgccgt taccggttcc gtcgcgaaca cgccaaatga cagagttgaa
    31921 agagccatag cagcaacaac agtattgata gttttcataa ttaatctctc gcaggtgatt
    31981 tttgtataag tgacgttgtt tcgtcgatgt gatgagtatc acgttttttt acgagagata
    32041 aaatcgaaga gaattgaccg ccttgttcaa ataaattgat tgatatctaa catactgata
    32101 aaccatttat ttgttgtact tatggacttg ttttatgccg cgtggcagtc acagcagcgt
    32161 taaaggcacg gaatgccacg caaaatacaa attatactcg ctatgcatct cgccagttgt
    32221 agttaacgta actgtctgtc tttcgaacct ctgcgattat atcctgtata cgctggtttt
    32281 tgtaaatctt tctcctgctg acaatgaata cataagcgca cgccaggaat ggcttcccga
    32341 cgggcttgcg ggatgggggc accgcactct tcacattcat acaggctttc gccacgcgga
    32401 atttcacccc gagcgcgggc aatcgcatct tcaattgtac tgttgatctg ttcgttgacg
    32461 gcgtcatcgt tagcccaagc ggatgccata tcgtcctccc catatcaatg cttgtacagt
    32521 taagtgtagc taatataggg acgaacctgg gcagttcaag cgtcagatct ccgaccattc
    32581 ccgcagcaga ttatgataaa gattgagcag cgacaggatc tcttcatttt caccatagcg
    32641 gcttttcagc gactgaatat ttttgtccag ttcaaacagc atggcgcgct ttttatcatc
    32701 gcggatcatc gactggatcc acataaatga tgccactcgt acgccgcggg ttacgggtgt
    32761 cacgcaatgc aggctgctgg agggatacaa caccagatca cctgccggga gttttacccg
    32821 atgttgtcca aaggtgtcat taacgaccag ttcgccgccg tcgtagcttt gtggatcgct
    32881 taaaaacagc gtggcagaaa ggtcagtgcg catccagccg ttttgtggat ggctgcgtac
    32941 tgcgccatcc acatggaaac cataggtttc attgttctga tagcgattaa acagcggcgt
    33001 ggaaagggta cgcggcaagg cagcggcaaa gaacaatgca tgttggttaa ccgcgttcag
    33061 cacctcattt tgcagtgcgg cgtataacgc gctgcgggtg tctacttgct gattgttctt
    33121 aacttgtgca ccttgtgctc cggtggtgac gcgtccatcc acccattcgg cttgttccag
    33181 ttgttcgcga aaatgggcga cgtcctgtgg cgataacacg ccgggaatgt ggtacatcat
    33241 acaagttctc caaaaagtgg ggcctgcgcc ccacatctga atcagaaatg catattggct
    33301 gtgagcaaga aggttcttgg ctcgcccggg tgataacggt agccgctctt gttgattgag
    33361 gcgacgtaat cggtatcaaa caggttgtaa acgtttagct ggaagtcgag attgcgatta
    33421 acccgatacc ccagtttggc atcggcgacc cagtaacctt ctgtaaatgc tggcgttccc
    33481 accgcgccgt ctgaaccttt atgtatactg ccgatatagc gtgcgcccgc gccaacagag
    33541 atatcatcgg ttgcctgata ttggctccat aaggtgaagg cgtgctccgg ggtatacggc
    33601 agcgatgagg aaccatcctg ggcaacatct ttgccgtttt tgatggttgc tttttgctgg
    33661 gtatagccgc caatcacctg ccacgcggga gtgatattcc ctgccacgga tatctcatag
    33721 ccttcgacgc gtttcttacc gtattgcgag taagtcccgt catcattttg ctcaacttca
    33781 ttttcgatat cagtacggaa cagcgcggcg gtgagcaaca ggcgtttatc cagaacctgc
    33841 catttggtgc caatctcgct ggtgttggct ttttgcggtt taaaatcggt gcggttggca
    33901 ctgttaccac tgccagactg cgcaagggcg aagttgttgc cgcccggagg ctgctgggaa
    33961 acggcatagt taatatagac attgccgttt tccgtcaggt ggtacagcgc cccggctttc
    34021 cagttcacca gattgcccga cttggcggtg tcgacggtgg tgactggaga accttttgcc
    34081 acaccagctg ggcaggtgat ggcaccgcgt ccgctgccgc cgcaggcggt ggcactgtca
    34141 tattcagtat gataattatc cagacggatc ccgccgttca gctcaaaatc acgggtgatt
    34201 tgcagcgtat cgaaggcgta aattgcgaag gtatccgtct gaccattggc gtttgcgccg
    34261 ttgcgcgtca ggccgccggg atgaattctg ctgtcaggat gataaatatt taccgccggt
    34321 aacgtcaccg gattaacgcc atagttagtc tgcgtttcac gggtaaattc cacgccggta
    34381 ctgacatcat gaccgataga accggtatag aacgtcgagg tcaggttggt ctggttggtg
    34441 agaattttat tactcacatc tttggtattc gccgtgcgtg actaggtcca gctattcaca
    34501 tcgctggtgg gctgagtaat attcgacgcc ccgcccataa tcgccgtcat caggtaatcc
    34561 tgctttacgc gcgaccaacg ggtagtattg cgaatggtgg tgttatcgtt gatgtcgtgc
    34621 tcaaaacgca tggtggcggt gtcggtggtc gaatcgtcgt aatcggaatc cgtgccgtaa
    34681 aagttatgag tatcaacttt tccggaatga ttcagggccg ccgctcccgc agatggggca
    34741 gaatagcccg gcaaaccgat ggtcggaatg ccgccgtctg gcgtgttgtg ctgagtgaca
    34801 tgcagataat taagatacaa acgatttgct gtaccaaggc caaaagcgac agaaggggcg
    34861 acgccgtaac gctcattttt gactttgtcg cgtccggcat cgtgggtttt ttcgcccatc
    34921 acattcaggc gcaccgcggt agtatcacca atgacctgat tgacgtccag cgtgccgcgg
    34981 cggaaccagg cgctgccaat actggcggag gcgtcaatgc cggaatcatt gcgcggctgc
    35041 ttgctgatca tattgatcga gcctgtcggt gcgctgcgcc cgtagtcggt gccggacggc
    35101 cctttaatca cttcgacctg ctcggtattg aaggtgtcgc gcgagacgct gccgatatcg
    35161 cgaataccat caatataaat actgttagag gtatcggcac cacgcatata aatggcgtcg
    35221 ccagtggtgg agttaccgtt ctcacccgca aaaaacgcac ccacgccgg
//
LOCUS       DAEEUE010000033        35155 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.033, whole genome shotgun sequence.
ACCESSION   DAEEUE010000033 DAEEUE010000000
VERSION     DAEEUE010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35155
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.033"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            88..1116
                     /gene="murB"
                     /locus_tag="KLK84_001766"
     CDS             88..1116
                     /gene="murB"
                     /locus_tag="KLK84_001766"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBE2009123.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
     gene            1113..2078
                     /gene="birA"
                     /locus_tag="KLK84_001767"
     CDS             1113..2078
                     /gene="birA"
                     /locus_tag="KLK84_001767"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HBE2009124.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGEMLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(2107..3057)
                     /gene="coaA"
                     /locus_tag="KLK84_001768"
                     /gene_synonym="panK"
     CDS             complement(2107..3057)
                     /gene="coaA"
                     /locus_tag="KLK84_001768"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBE2009125.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            3419..3494
                     /locus_tag="KLK84_001769"
     tRNA            3419..3494
                     /locus_tag="KLK84_001769"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3452..3454,aa:Thr,seq:tgt)
     gene            3503..3587
                     /locus_tag="KLK84_001770"
     tRNA            3503..3587
                     /locus_tag="KLK84_001770"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3537..3539,aa:Tyr,seq:gta)
     gene            3704..3778
                     /locus_tag="KLK84_001771"
     tRNA            3704..3778
                     /locus_tag="KLK84_001771"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3737..3739,aa:Gly,seq:tcc)
     gene            3785..3860
                     /locus_tag="KLK84_001772"
     tRNA            3785..3860
                     /locus_tag="KLK84_001772"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3818..3820,aa:Thr,seq:ggt)
     gene            3975..5159
                     /gene="tuf"
                     /locus_tag="KLK84_001773"
     CDS             3975..5159
                     /gene="tuf"
                     /locus_tag="KLK84_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBE2009126.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVG
                     VLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFR
                     TTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKV
                     LS"
     gene            5389..5772
                     /gene="secE"
                     /locus_tag="KLK84_001774"
     CDS             5389..5772
                     /gene="secE"
                     /locus_tag="KLK84_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBE2009127.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPIRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            5774..6319
                     /gene="nusG"
                     /locus_tag="KLK84_001775"
     CDS             5774..6319
                     /gene="nusG"
                     /locus_tag="KLK84_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBE2009128.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            6478..6906
                     /gene="rplK"
                     /locus_tag="KLK84_001776"
     CDS             6478..6906
                     /gene="rplK"
                     /locus_tag="KLK84_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBE2009129.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            6910..7614
                     /gene="rplA"
                     /locus_tag="KLK84_001777"
     CDS             6910..7614
                     /gene="rplA"
                     /locus_tag="KLK84_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBE2009130.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            8027..8524
                     /gene="rplJ"
                     /locus_tag="KLK84_001778"
     CDS             8027..8524
                     /gene="rplJ"
                     /locus_tag="KLK84_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBE2009131.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            8591..8956
                     /gene="rplL"
                     /locus_tag="KLK84_001779"
     CDS             8591..8956
                     /gene="rplL"
                     /locus_tag="KLK84_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBE2009132.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            9276..13304
                     /gene="rpoB"
                     /locus_tag="KLK84_001780"
     CDS             9276..13304
                     /gene="rpoB"
                     /locus_tag="KLK84_001780"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBE2009133.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            13381..17604
                     /gene="rpoC"
                     /locus_tag="KLK84_001781"
     CDS             13381..17604
                     /gene="rpoC"
                     /locus_tag="KLK84_001781"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBE2009134.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(17846..18979)
                     /gene="thiH"
                     /locus_tag="KLK84_001782"
     CDS             complement(17846..18979)
                     /gene="thiH"
                     /locus_tag="KLK84_001782"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBE2009135.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(18976..19746)
                     /gene="thiG"
                     /locus_tag="KLK84_001783"
     CDS             complement(18976..19746)
                     /gene="thiG"
                     /locus_tag="KLK84_001783"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBE2009136.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(19748..19948)
                     /gene="thiS"
                     /locus_tag="KLK84_001784"
     CDS             complement(19748..19948)
                     /gene="thiS"
                     /locus_tag="KLK84_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBE2009137.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(19932..20687)
                     /gene="thiF"
                     /locus_tag="KLK84_001785"
     CDS             complement(19932..20687)
                     /gene="thiF"
                     /locus_tag="KLK84_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HBE2009138.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(20680..21315)
                     /gene="thiE"
                     /locus_tag="KLK84_001786"
     CDS             complement(20680..21315)
                     /gene="thiE"
                     /locus_tag="KLK84_001786"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBE2009139.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(21315..23210)
                     /gene="thiC"
                     /locus_tag="KLK84_001787"
     CDS             complement(21315..23210)
                     /gene="thiC"
                     /locus_tag="KLK84_001787"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBE2009140.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     DMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(23291..23391)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(23444..23920)
                     /gene="rsd"
                     /locus_tag="KLK84_001788"
     CDS             complement(23444..23920)
                     /gene="rsd"
                     /locus_tag="KLK84_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HBE2009141.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            24015..24788
                     /gene="nudC"
                     /locus_tag="KLK84_001789"
     CDS             24015..24788
                     /gene="nudC"
                     /locus_tag="KLK84_001789"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HBE2009142.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            24828..25892
                     /gene="hemE"
                     /locus_tag="KLK84_001790"
     CDS             24828..25892
                     /gene="hemE"
                     /locus_tag="KLK84_001790"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HBE2009143.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            26049..26720
                     /gene="nfi"
                     /locus_tag="KLK84_001791"
     CDS             26049..26720
                     /gene="nfi"
                     /locus_tag="KLK84_001791"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HBE2009144.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            26763..27353
                     /locus_tag="KLK84_001792"
     CDS             26763..27353
                     /locus_tag="KLK84_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HBE2009145.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            27540..27812
                     /gene="hupA"
                     /locus_tag="KLK84_001793"
     CDS             27540..27812
                     /gene="hupA"
                     /locus_tag="KLK84_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HBE2009146.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            27825..28520
                     /locus_tag="KLK84_001794"
     CDS             27825..28520
                     /locus_tag="KLK84_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HBE2009147.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(28522..28947)
                     /gene="zraP"
                     /locus_tag="KLK84_001795"
     CDS             complement(28522..28947)
                     /gene="zraP"
                     /locus_tag="KLK84_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HBE2009148.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            29185..30561
                     /gene="zraS"
                     /locus_tag="KLK84_001796"
     CDS             29185..30561
                     /gene="zraS"
                     /locus_tag="KLK84_001796"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HBE2009149.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATLLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            30558..31883
                     /gene="zraR"
                     /locus_tag="KLK84_001797"
     CDS             30558..31883
                     /gene="zraR"
                     /locus_tag="KLK84_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HBE2009150.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWSYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(31880..33169)
                     /gene="purD"
                     /locus_tag="KLK84_001798"
     CDS             complement(31880..33169)
                     /gene="purD"
                     /locus_tag="KLK84_001798"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBE2009151.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(33181..34770)
                     /gene="purH"
                     /locus_tag="KLK84_001799"
     CDS             complement(33181..34770)
                     /gene="purH"
                     /locus_tag="KLK84_001799"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBE2009152.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         8384 a         8997 c         9570 g         8204 t
ORIGIN      
        1 ctacaaactc ttcctgtcgt catatctaca agccatcccc ccacagatac ggtaaactag
       61 cctcgttttt gcatcaggaa agcagctatg aaccactcct taaaaccctg gaacacattt
      121 ggcattgatc ataatgctca gcacattgta tgtgccgaag acgaacaaca attactcaat
      181 gcctggcagc atgcaaccgc agaaggacaa cccgttctta ttctgggtga aggaagtaat
      241 gtactttttc tggaagacta tcgcggcacg gtgatcatca accggatcaa aggtatcgaa
      301 attcatgatg aacctgatgc gtggtattta catgtaggag ccggagaaaa ctggcatcgc
      361 ctggtaaaat acactttgca ggaaggtatg cctggtctgg aaaatctggc attaattcct
      421 ggttgtgtcg gctcatcacc tatccagaat attggtgctt atggcgtaga attacagcga
      481 gtttgcgctt atgttgattg tgttgaactg gcgacaggca agcaagtgcg cttaactgcc
      541 aaagagtgcc gttttggcta tcgcgacagt atttttaaac atgaatacca ggatcgcttc
      601 gccattgtag ccgtaggtct gcgtctgcca aaagagtggc aacctgtact aacgtatggt
      661 gacttaactc gtctggatcc tacaacagta acgccacagc aagtatttaa tgcggtgtgt
      721 catatgcgca ccaccaaact ccctgatcca aaagtgaatg gcaatgccgg tagtttcttc
      781 aaaaaccctg ttgtatctgc cgaaacggct aaagcattac tggcgcaatt tccaacagca
      841 ccaaattacc cccaggcggg tggttcagta aaactggcag caggttggct tattgatcag
      901 tgccagctaa aagggatgca aatgggtggg gctgcggtgc accgtcaaca ggcgttagtt
      961 ctcattaatg aagacaatgc aaaaagcgaa gatgtggtgc aactggcaca ccatgtaaga
     1021 caaaaagtgg gtgaaaaatt taatgtctgg cttgagcctg aagtccgctt tattggtgca
     1081 tcaggtgaag tgagcgcagt ggagacaatt tcatgaagga taacaccgtg ccactgaaat
     1141 tgattgccct gttagcgaac ggtgaatttc actctggcga gcagttgggt gaaatgctgg
     1201 gaatgagccg ggcggctatt aataaacaca ttcagacact gcgtgactgg ggcgttgatg
     1261 tctttaccgt tccgggtaaa ggatacagcc tgcctgagcc catccagtta cttaatgctg
     1321 aacagatatt gggtcagctg gatggcggta gtgtagccgt gctgccagtt attgactcca
     1381 cgaatcagtt ccttcttgat cgtatcggag agcttaaatc gggcgatgcc tgtgttgcag
     1441 aataccagca ggctggccgt ggtcgccggg ggcggaaatg gttttcgcct tttggcgcaa
     1501 acttatattt gtcgatgttc tggcgtctgg aacaaggccc ggcggcggcg attggtttaa
     1561 gtctggttat cggtatcgtg atggcggaag tattacgaaa gctgggagca gataaagttc
     1621 gtgtcaaatg gcctaatgac ctctatctgc aggatcgcaa gctggcaggc attcttgtgg
     1681 agctgactgg caaaactggc gatgcggcgc aaatagtcat tggagccggg atcaacatgg
     1741 caatgcgccg tgttgaagag agtgtcgtta atcaggggtg gatcacgctg caggaagcgg
     1801 ggatcaatct tgatcgtaat acgttggcgg ccatgctaat acgtgaatta cgtgctgcgt
     1861 tggaactctt cgaacaagaa ggattggcac cttatctgtc gcgctgggaa aagctggata
     1921 attttattaa tcgcccagtg aaacttatca ttggtgataa agaaatattt ggcatttcac
     1981 gcggaataga caaacagggg gctttattac ttgagcagga tggaataata aaaccctgga
     2041 tgggcggtga aatatccctg cgtagtgcag aaaaataaga aaggggagta ttcgctcccc
     2101 tgcaaattat ttgcgtagtc tgacctcttc taccacatga ttagcacttt tcgtcaggat
     2161 taaactggcg cgctcacgag taggtagaat attttgcttt aagttcagcc agttgatctc
     2221 tttccacaat gtcatggcag tcttaatcgc ttcttcttta gttaatttcg cgtagttatg
     2281 aaaataggaa tccgggtctg taaaagcccc ttcgcggaat ttcagaaaac ggttgatata
     2341 ccacgtctga agtaagtctt ccggtgcatc aacatatatc gaaaaatcga caaaatcgga
     2401 aacaaataca tgatgtggat cgtgtggata atccatcccg ctctgtaaga catttaaccc
     2461 ttcaagaatt aaaatatcag gctgaacaac agttttatct ccatccggga tcacatcata
     2521 aataagatgc gagtaaacag gggctgtaac gtttggcacg ccggatttga gatcggaaac
     2581 aaacttcacc aggcgatgca tatcatacga ttccgggaag cctttcttct tcatcagacc
     2641 acgttctttc agaacctgat tagggtgaag gaagccatct gtagtgatca gttcaacacg
     2701 acgatgttcc ggccaacggc ttaatagcgc ctgcaataca cgggcggttg tacttttccc
     2761 caccgcgaca ctgccagcaa tactgataat gtaaggaatg cgttgcccgt tggtaccaag
     2821 aaactgttcc agaactgcct gacggcgcag attcgagctt atatagaagt tcagcaaacg
     2881 tgacaaaggt aaatagatct cggcaacttc ttctaacgag agatcttcat taataccttt
     2941 gagacgggcg atctcatctt ccgataacgt cataggtacg gaatcacgca gagctgccca
     3001 ctggttgcgg tcaaactgta ggtaaggcgt cattaacgtt tgctctttta tactcataag
     3061 catgtttctg gcggtcatag ctctatcggc aaagcgatat ttgccatggc gcgtcacagt
     3121 tctgttgaat gggttaatgg gcaggagggt aacaccagat ggggaggaat aataagaaaa
     3181 aatctctgcc aggaagctat cgttgaaaag caatgtgaca gatattccat ctgataaaaa
     3241 gaattatggt ttagcaggag cgcattgttg agcacaatga tgttgaaaaa gtgtgctaat
     3301 ctgccctccg ttcggctgtt tcttcatcgt gtagcataaa atgtgaccaa taaaacaaat
     3361 tatgcaattt tttagttgca tgaactcgca tgtctccata gaatgcgcgc tacttgatgc
     3421 cgacttagct cagtaggtag agcaactgac ttgtaatcag taggtcacca gttcgattcc
     3481 ggtagtcggc accatcaagt ccggtggggt tcccgagcgg ccaaagggag cagactgtaa
     3541 atctgccgtc acagacttcg aaggttcgaa tccttccccc accaccaatt tcggccacgc
     3601 gatggcgtag cccgagacga taagttcact taccggctcg aataaagaga gcttctctcg
     3661 atattcagtg cagaatgaaa atcaggtagc cgagttctag gatgcgggca tcgtataatg
     3721 gctattacct cagccttcca agctgatgat gcgggttcga ttcccgctgc ccgctccaag
     3781 atgtgctgat atagctcagt tggtagagcg cacccttggt aagggtgagg tcggcagttc
     3841 gaatctgcct atcagcacca cttcttttct cctccctgtt ttttccttct gtttattgca
     3901 ttcaacaagt cgggcatgtt gcctggttga tgtggtgata tcaccgattt atccgtgtct
     3961 tagagggaca atcgatgtct aaagaaaagt ttgaacgtac aaaaccgcac gttaacgtcg
     4021 gtactatcgg ccacgttgac catggtaaaa caacgctgac cgctgcaatc actaccgtac
     4081 tggctaaaac ctacggcggt gctgctcgcg cattcgacca gatcgataac gcgccggaag
     4141 aaaaagctcg tggtatcacc atcaacactt ctcacgttga atacgacacc ccgacccgtc
     4201 actacgcaca cgtagactgc ccggggcacg ccgactatgt taaaaacatg atcaccggtg
     4261 ctgctcagat ggacggcgcg atcctggtag ttgctgcgac tgacggcccg atgccgcaga
     4321 ctcgtgagca catcctgctg ggtcgtcagg taggcgttcc gtacatcatc gtgttcctga
     4381 acaaatgcga catggttgat gacgaagagc tgctggaact ggttgaaatg gaagttcgtg
     4441 aacttctgtc tcagtacgac ttcccgggcg acgacactcc gatcgttcgt ggttctgctc
     4501 tgaaagcgct ggaaggcgac gcagagtggg aagcgaaaat cctggaactg gctggcttcc
     4561 tggattctta cattccggaa ccagagcgtg cgattgacaa gccgttcctg ctgccgatcg
     4621 aagacgtatt ctccatctcc ggtcgtggta ccgttgttac cggtcgtgta gaacgcggca
     4681 tcatcaaagt tggtgaagaa gttgaaatcg ttggtatcaa agagactcag aagtctacct
     4741 gtactggcgt tgaaatgttc cgcaaactgc tggacgaagg gcgtgctggt gagaacgtag
     4801 gtgttctgct gcgtggtatc aaacgtgaag aaatcgaacg tggtcaggta ctggctaagc
     4861 cgggcaccat caagccgcac accaagttcg aatctgaagt gtacattctg tccaaagatg
     4921 aaggcggtcg tcatactccg ttcttcaaag gctaccgtcc gcagttctac ttccgtacta
     4981 ctgacgtgac tggtaccatc gaactgccgg aaggcgtaga gatggtaatg ccgggcgaca
     5041 acatcaaaat ggttgttacc ctgatccacc cgatcgcgat ggacgacggt ctgcgtttcg
     5101 caatccgtga aggcggccgt accgttggtg cgggcgttgt agcaaaagtt ctgagctaat
     5161 tgccgataac atttgacgca atgcgcacta aaagggcatc atttgatgcc ctttttgcac
     5221 gctttcgtac cagaacctgg ctcatcagtg attttctttg tcataatcat tgctgagaca
     5281 ggctctgttg agggcgtata atccgaaaag ctaatacgcg tttcgatttg gtttgcctcg
     5341 cgatcgcggg gtgaaaatgt ttgtagaaaa cttctgacag gttggtttat gagtgcgaat
     5401 accgaagctc aaggaagcgg gcgcggcctg gaagcgatga agtgggtcgt tgtggtggca
     5461 ttgctcctgg cggcgattgt cggcaactac ctttatcgcg acattatgct gccgctgcgt
     5521 gcgctggccg tagtaattct gattgctgca gcgggtggtg tcgcgctgtt aacgacaaaa
     5581 ggtaaagcta ccattgcttt tgcccgtgaa gcgcgtaccg aagtccgtaa ggtcatttgg
     5641 ccgattcgcc aggaaacatt gcacaccacg ctgattgtgg ctgcggttac cgcagtaatg
     5701 tcactgatcc tgtggggact ggatggtatt ctggttcgcc tggtatcctt tatcactggc
     5761 ctgaggttct gagatgtctg aagctcctaa aaagcgctgg tacgtcgttc aggcgttttc
     5821 cggttttgaa ggccgcgtag caacgtcgct gcgtgagcat atcaaattac acaacatgga
     5881 agatttgttt ggtgaagtca tggtaccaac cgaagaagtg gttgaaatcc gtggcggtca
     5941 gcgtcgcaaa agcgaacgta aattcttccc tggctacgtc ctcgttcaga tggtgatgaa
     6001 cgacgcgagc tggcacttgg tgcgcagcgt accgcgtgtg atgggcttca tcggcggtac
     6061 ttccgatcgt cctgcgccaa tcagcgataa agaagtcgat gcgattatga accgcctgca
     6121 gcaggttggt gataagccgc gtccgaaaac gctgtttgaa ccgggtgaaa tggtccgtgt
     6181 taatgatggt ccgttcgctg acttcaacgg tgttgttgaa gaagtggatt acgagaaatc
     6241 tcgtctgaaa gtgtctgttt ctatcttcgg tcgtgcgacc ccggtagagc tggacttcag
     6301 ccaggttgaa aaagcctaac ccagcgatca aaaaagcggc gatttaatcg ttgcacaagg
     6361 cgtgagattg gaatacaatt tcgcgccttt tgtttttatg ggccttgccc gtaaaacgat
     6421 tttttatatc acggggagcc tctcagaggc gttattaccc aacttgagga atttataatg
     6481 gctaagaaag tacaagccta tgtcaagctg caggttgcag ctggtatggc taacccgagt
     6541 ccgccagtag gtccggctct gggtcagcag ggcgtaaaca tcatggaatt ctgcaaagcg
     6601 ttcaacgcaa aaactgattc catcgaaaaa ggtctgccga ttccggtagt aatcaccgtt
     6661 tacgctgacc gttctttcac tttcgttacc aagaccccgc cggcagcagt tctgctgaaa
     6721 aaagcggcag gtatcaagtc tggttccggt aagccgaaca aagacaaagt gggtaaaatt
     6781 tcccgcgctc agctgcagga aatcgcgcag accaaagctg ccgacatgac tggtgccgac
     6841 attgaagcga tgactcgctc catcgaaggt actgcacgtt ccatgggcct ggtagtggag
     6901 gactaagaaa tggctaaact gaccaagcgc atgcgtgtta tccgtgagaa agttgatgca
     6961 accaaacagt acgacatcaa cgaagctatc gcactgctga aagagctggc gactgctaaa
     7021 ttcgtagaaa gcgtggacgt agctgttaac ctcggcatcg acgctcgtaa atctgaccag
     7081 aacgtacgtg gtgcaactgt actgccgcac ggtactggcc gttccgttcg cgtagccgta
     7141 tttacccaag gtgcaaacgc tgaagctgct aaagctgcag gcgcagaact ggtaggtatg
     7201 gaagatctgg ctgaccagat caagaaaggc gaaatgaact ttgacgttgt tattgcttct
     7261 ccggatgcaa tgcgcgttgt tggccagctg ggccaggttc tgggtccgcg tggcctgatg
     7321 ccaaacccga aagtgggtac tgtaacaccg aacgttgctg aagcggttaa aaacgctaaa
     7381 gctggccagg ttcgttaccg taacgacaaa aacggcatca tccacaccac catcggtaaa
     7441 gtggactttg acgctgacaa actgaaagaa aacctggaag ctctgctggt tgcgctgaaa
     7501 aaagcaaaac cgactcaggc gaaaggcgtg tacatcaaga aagttagcat ctccaccacc
     7561 atgggtgcag gtgttgcagt tgaccaggct ggcctgagcg cttctgtaaa ctaatgcctt
     7621 tacgtgggcg gtgattttgt ctacaatctt acccccacgt ataatgctta atgcagacgt
     7681 atatccgaga tattcgggtt gtggcaaggc ggcaactgag tgagtcgcca agagcatagc
     7741 taactatgtg actggtgcga atgaaggaag ccaacgccgc cacaagttga atagcgacgg
     7801 atagaaaaga tttgttcgtt ggagcctggc ctatccaggc ctccgtcgaa gaccgcagga
     7861 gtttcgcaag aaacttaatc ccctgcgtag acggtgacag aacgctaaga ttattctttt
     7921 atattctggc ttgtttctgc tcaccgtaat taagacgctc tctccgtttg gaggagtgaa
     7981 gtgagttcca gagattttct ctggcaaaca tccaggagca aagctaatgg ctttaaatct
     8041 tcaagacaaa caagcgattg ttgctgaagt cagcgaagta gccaaaggcg cgctgtctgc
     8101 agtagttgcg gattcccgtg gcgtaactgt agataaaatg actgaactgc gtaaagcagg
     8161 tcgcgaagct ggcgtataca tgcgtgttgt tcgtaacacc ctgctgcgcc gtgctgttga
     8221 aggtactccg ttcgagtgcc tgaaagacgc gtttgttggt ccgaccctga ttgcatactc
     8281 tatggaacac ccgggcgctg ctgctcgtct gttcaaagag ttcgcgaaag cgaatgcaaa
     8341 atttgaggtc aaagccgctg cctttgaagg tgagctgatc ccggcgtctc agatcgaccg
     8401 cctggcaact ctgccgacct acgaagaagc aattgcacgc ctgatggcaa ccatgaaaga
     8461 agcttcggct ggcaaactgg ttcgtactct ggctgctgta cgcgatgcga aagaagctgc
     8521 ttaatcgcag ttatcttttt aacgcattcg cttacgtata aacttattct gatattcagg
     8581 aacaatttaa atgtctatca ctaaagatca aatcattgaa gcagttgcag ctatgtctgt
     8641 aatggacgtt gtagaactga tctctgcaat ggaagaaaaa ttcggtgttt ccgctgctgc
     8701 tgctgtagct gtagctgctg gcccggttga agctgctgaa gaaaaaactg aattcgacgt
     8761 aattctgaaa gctgctggcg ctaacaaagt tgctgttatc aaagcagtac gtggcgcaac
     8821 tggcctgggt ctgaaagaag ctaaagactt ggtagaatct gcaccggctg ctctgaaaga
     8881 aggcgtgagc aaagacgacg cagaagcact gaaaaaagct ctggaagaag ctggcgctga
     8941 agttgaagtt aaataagcca acccttccgg ttgcagcctg agaaatcagg ctgatggctg
     9001 gtgacttttt agtcaccagc ctttttgcgc tgtaaggcgc cagtagcgtt tcacactgtt
     9061 tgactactgc tgtgcctttc aatgcttgtt tctatcgacg acttaatata ctgcgacagg
     9121 acgtccgttc tgtgtaaatc gcaatgaaat ggtttaagcg tgatagcaac aggcattgcg
     9181 gaaagtgttc cattttccgg tcaacaaaat agtgttgcac aaactgtccg ctcaatggac
     9241 agatgggtcg acttgtcagc gagctgagga accctatggt ttactcctat accgagaaaa
     9301 aacgtattcg taaggatttt ggtaaacgtc cacaagttct ggatgtacct tatctccttt
     9361 ctatccagct tgactcgttt cagaaattta tcgagcaaga tcctgaaggg cagtatggtc
     9421 tggaagctgc tttccgttcc gtattcccga ttcagagcta cagcggtaat tccgagctgc
     9481 aatacgtcag ctaccgcctt ggcgaaccgg tgtttgacgt ccaggaatgt caaatccgtg
     9541 gcgtgaccta ttccgcaccg ctgcgcgtta aactgcgtct ggtgatctat gagcgcgaag
     9601 cgccggaagg caccgtaaaa gacattaaag aacaagaagt ctacatgggc gaaattccgc
     9661 tcatgacaga caacggtacc tttgttatca acggtactga gcgtgttatc gtttcccagc
     9721 tgcaccgtag tccgggcgtc ttctttgact ccgacaaagg taaaacccac tcttcgggta
     9781 aagtgctgta taacgcgcgc atcatccctt accgtggttc ctggctggac ttcgaattcg
     9841 atccgaagga caacctgttc gtacgtatcg accgtcgccg taaactgcct gcgaccatca
     9901 ttctgcgcgc cctgaactac accacagagc agatcctcga cctgttcttt gaaaaagtta
     9961 tctttgaaat ccgtgataac aagctgcaga tggaactggt gccggaacgc ctgcgtggtg
    10021 aaaccgcatc ttttgacatc gaagctaacg gtaaagtgta cgtagaaaaa ggccgccgta
    10081 tcactgcgcg ccacattcgc cagctggaaa aagacgacgt caaactgatc gaagtcccgg
    10141 ttgagtacat cgcaggtaaa gtggttgcta aagactatat tgatgagtct accggcgagc
    10201 tgatctgcgc agcgaacatg gagctgagcc tggatctgct ggctaagctg agccagtctg
    10261 gtcacaagcg tatcgaaacg ctgttcacca acgatctgga tcacggccca tatatctctg
    10321 aaaccttacg tgtcgaccca actaacgacc gtctgagcgc actggtagaa atctaccgca
    10381 tgatgcgccc tggcgagccg ccgactcgtg aagcagctga aagcctgttc gagaacctgt
    10441 tcttctccga agaccgttat gacttgtctg cggttggtcg tatgaagttc aaccgttctc
    10501 tgctgcgcga agaaatcgaa ggttccggta ttctgagcaa agacgacatc attgatgtta
    10561 tgaaaaagct catcgatatc cgtaacggta aaggcgaagt cgatgatatc gaccacctcg
    10621 gcaaccgtcg tatccgttcc gttggcgaaa tggcggaaaa ccagttccgc gttggcttgg
    10681 tacgtgtaga gcgtgcggtg aaagagcgtc tgtctctggg cgatctggat accctgatgc
    10741 ctcaggatat gatcaacgcc aagccgattt ccgcagcagt gaaagagttc ttcggttcca
    10801 gccagctgtc tcagtttatg gaccagaaca acccgctgtc tgagattacg cacaaacgtc
    10861 gtatctccgc actcggccca ggcggtctga cccgtgaacg tgcaggcttc gaagttcgag
    10921 acgtacaccc gactcactac ggtcgcgtat gtccaatcga aacccctgaa ggtccgaaca
    10981 tcggtctgat caactctctg tccgtgtacg cacagactaa cgaatacggc ttccttgaga
    11041 ctccgtatcg taaagtgacc gacggtgttg taactgacga aattcactac ctgtctgcta
    11101 tcgaagaagg caactacgtt atcgcccagg cgaactccaa cctggatgaa gaaggccact
    11161 tcgtagaaga cctggtaact tgccgtagca aaggcgaatc cagcttgttc agccgcgacc
    11221 aggttgacta catggacgta tccacccagc aggtggtatc cgtcggtgcg tccctgatcc
    11281 cgttcctgga acacgatgac gccaaccgtg cattgatggg tgcgaatatg caacgtcagg
    11341 ccgttccgac tctgcgcgct gataagccgc tggttggtac tggtatggaa cgtgctgttg
    11401 ccgttgactc cggtgtaact gcggtagcta aacgtggtgg tgtcgttcag tacgtggatg
    11461 cttcccgtat cgttatcaaa gttaacgaag acgagatgta tccgggtgaa gcaggtatcg
    11521 acatctacaa cctgaccaaa tacacccgtt ctaaccagaa cacctgtatc aaccagatgc
    11581 cgtgtgtgtc tctgggtgaa ccggttgaac gtggcgacgt gctggcagac ggtccgtcca
    11641 ccgacctcgg tgaactggcg cttggtcaga acatgcgcgt agcgttcatg ccgtggaatg
    11701 gttacaactt cgaagactcc atcctcgtat ccgagcgtgt tgttcaggaa gaccgtttca
    11761 ccaccatcca cattcaggaa ctggcgtgtg tgtcccgtga caccaagctg ggtccggaag
    11821 agatcaccgc tgacatcccg aacgtgggtg aagctgcgct ctccaaactg gatgaatccg
    11881 gtatcgttta cattggtgcg gaagtgaccg gtggcgacat tctggttggt aaggtaacgc
    11941 cgaaaggtga aactcagctg accccagaag aaaaactgct gcgtgcgatc ttcggtgaga
    12001 aagcctctga cgttaaagac tcttctctgc gcgtaccaaa cggtgtatcc ggtacggtta
    12061 tcgacgttca ggtctttact cgcgatggcg tagaaaaaga caaacgtgcg ctggaaatcg
    12121 aagaaatgca gctcaaacag gcgaagaaag acctgtctga agaactgcag atcctcgaag
    12181 cgggtctgtt cagccgtatc cgtgctgtgc tggtagccgg tggcgttgaa gctgagaagc
    12241 tcgacaaact gccgcgcgat cgctggctgg agctgggcct gacagacgaa gagaaacaaa
    12301 atcagctgga acagctggct gagcagtatg acgaactgaa acacgagttc gagaagaaac
    12361 tcgaagcgaa acgccgcaaa atcacccagg gcgacgatct ggcaccgggc gtgctgaaga
    12421 ttgttaaggt atatctggcg gttaaacgcc gtatccagcc tggtgacaag atggcaggtc
    12481 gtcacggtaa caagggtgta atttctaaga tcaacccgat cgaagatatg ccttacgatg
    12541 aaaacggtac gccggtagac atcgtactga acccgctggg cgtaccgtct cgtatgaaca
    12601 tcggtcagat cctcgaaacc cacctgggta tggctgcgaa aggtatcggc gacaagatca
    12661 acgccatgct gaaacagcag caagaagtcg cgaaactgcg cgaattcatc cagcgtgcgt
    12721 acgatctggg cgctgacgtt cgtcagaaag ttgacctgag taccttcagc gatgaagaag
    12781 ttatgcgtct ggctgaaaac ctgcgcaaag gtatgccaat cgcaacgccg gtgttcgacg
    12841 gtgcgaaaga agcagaaatt aaagagctgc tgaaacttgg cgacctgccg acttccggtc
    12901 agatccgcct gtacgatggt cgcactggtg aacagttcga gcgtccggta accgttggtt
    12961 acatgtacat gctgaaactg aaccacctgg tcgacgacaa gatgcacgcg cgttccaccg
    13021 gttcttacag cctggttact cagcagccgc tgggtggtaa ggcacagttc ggtggtcagc
    13081 gtttcgggga gatggaagtg tgggcgctgg aagcatacgg cgcagcatac accctgcagg
    13141 aaatgctcac tgttaagtct gatgacgtga acggtcgtac caagatgtat aaaaacatcg
    13201 tggacggcaa ccatcagatg gagccgggca tgccagaatc cttcaacgta ttgttgaaag
    13261 agattcgttc gctgggtatc aacatcgaac tggaagacga gtaattctcg ctcaaacagg
    13321 tcactgctgt cggggtaaaa cccggcagcg gattgtgcta actccgacgg gagcaaatcc
    13381 gtgaaagatt tattaaagtt tctgaaagcg cagactaaaa ccgaagagtt tgatgcgatc
    13441 aaaattgctc tggcttcgcc agacatgatc cgttcatggt ctttcggtga agttaaaaag
    13501 ccggaaacca tcaactaccg tacgttcaaa ccagaacgtg acggcctttt ctgcgcccgt
    13561 atctttgggc cggtaaaaga ttacgagtgc ctgtgcggta agtacaagcg cctgaaacac
    13621 cgtggcgtca tctgtgagaa gtgcggcgtt gaagtgaccc agactaaagt acgccgtgag
    13681 cgtatgggcc acatcgaact ggcttccccg actgcgcaca tctggttcct gaaatcgctg
    13741 ccgtcccgta tcggtctgct gctcgatatg ccgctgcgcg atatcgaacg cgtactgtac
    13801 tttgaatcct atgtggttat cgaaggcggt atgaccaacc tggaacgtca gcagatcctg
    13861 actgaagagc agtatctgga cgcgctggaa gagttcggtg acgaattcga cgcgaagatg
    13921 ggtgcggaag caatccaggc tctgctgaag agcatggatc tggagcaaga gtgcgaacag
    13981 ctgcgtgaag agctgaacga aaccaactcc gaaaccaagc gtaaaaagct gaccaagcgt
    14041 atcaaactgc tggaagcgtt cgttcagtct ggtaacaaac cagagtggat gatcctgacc
    14101 gttctgccgg tactgccgcc agatctgcgt ccgctggttc cgctggatgg tggtcgtttc
    14161 gcgacttctg acctgaacga tctgtatcgt cgcgtcatta accgtaacaa ccgtctgaaa
    14221 cgtctgctgg atctggctgc gccggacatc atcgtacgta acgaaaaacg tatgctgcag
    14281 gaagcggtag acgccctgct ggataacggt cgtcgcggtc gtgcgatcac cggttctaac
    14341 aagcgtcctc tgaaatcttt ggccgacatg atcaaaggta aacagggtcg tttccgtcag
    14401 aacctgctcg gtaagcgtgt tgactactcc ggtcgttctg taatcaccgt aggtccatac
    14461 ctgcgtctgc atcagtgcgg tctgccgaag aaaatggcgc tggagctgtt caaaccgttc
    14521 atctacggca agctggaact gcgtggtctt gctaccacca ttaaagctgc gaagaaaatg
    14581 gttgagcgcg aagaagctgt cgtttgggat atcctggacg aagttatccg cgaacacccg
    14641 gtactgctga accgtgcacc gactctgcac cgtctgggta tccaggcatt tgaaccggta
    14701 ctgatcgaag gtaaagctat ccagctgcac ccgctggttt gtgcggcata taacgccgac
    14761 ttcgatggtg accagatggc tgttcacgta ccgctgacgc tggaagccca gctggaagcg
    14821 cgtgcgctga tgatgtctac caacaacatc ctgtccccgg cgaacggcga accaatcatc
    14881 gttccgtctc aggacgttgt actgggtctg tactacatga cccgtgactg tgttaacgcc
    14941 aaaggcgaag gcatggtgct gactggcccg aaagaagcag aacgtctgta tcgctctggt
    15001 ctggcttctc tgcatgcgcg cgttaaagtg cgtatcaccg agtatgaaaa agatgctaac
    15061 ggtgaattag tagcgaaaac cagcctgaaa gacacgactg ttggccgtgc cattctgtgg
    15121 atgattgtac cgaaaggtct gccttactcc atcgtcaacc aggcgctggg taaaaaagca
    15181 atctccaaaa tgctgaacac ctgctaccgc attctcggtc tgaaaccgac cgttattttt
    15241 gcggaccaga tcatgtacac cggcttcgcc tatgcagcgc gttctggtgc atctgttggc
    15301 atcgatgaca tggtcatccc ggagaagaaa cacgaaatca tctccgaggc agaagcagaa
    15361 gttgctgaaa ttcaggagca gttccagtct ggtctggtaa ctgcgggcga acgctacaac
    15421 aaagttatcg atatctgggc tgcggcgaac gatcgtgtat ccaaagcgat gatggataac
    15481 ctgcaaactg aaaccgttat taaccgtgac ggtcaggaag agaagcaggt ttccttcaac
    15541 agcatctaca tgatggccga ctccggtgcg cgtggttctg cggcacagat tcgtcagctt
    15601 gctggtatgc gtggtctgat ggcaaagccg gatggctcca tcatcgaaac gccaatcacc
    15661 gcgaacttcc gtgaaggtct gaacgtactc cagtacttca tctccaccca cggtgctcgt
    15721 aaaggtctgg cggataccgc actgaaaact gcgaactccg gttacctgac tcgtcgtctg
    15781 gttgacgtgg cgcaggacct ggtggttacc gaagacgatt gtggtaccca tgaaggtatc
    15841 atgatgactc cggttatcga ggggggtgac gttaaagagc cgctgcgcga tcgcgtactg
    15901 ggtcgtgtaa ctgctgaaga cgttctgaag ccgggtactg ctgatattct cgttccgcgc
    15961 aacacgctgc tgcacgaaca gtggtgtgac ctgctggaag agaactctgt cgacgcggta
    16021 aaagtacgtt ctgttgtatc ttgtgacacc gactttggtg tatgtgcgca ctgctacggt
    16081 cgtgacctgg cgcgtggcca catcatcaac aaaggtgaag caatcggtgt tatcgcggca
    16141 cagtccatcg gtgaaccggg tacacagctg accatgcgta cgttccacat cggtggtgcg
    16201 gcatctcgtg cggctgctga atccagcatc caggtgaaaa acaaaggtag catcaagctc
    16261 agcaacgtga agtcggttgt gaactccagc ggtaaactgg ttatcacttc ccgtaacacc
    16321 gaactgaaac tgatcgacga attcggtcgt accaaagaaa gctacaaagt gccttacggt
    16381 gcggtactgg cgaaaggcga tggcgaacag gttgctggcg gcgaaaccgt tgcaaactgg
    16441 gacccgcaca ccatgccggt tatcaccgaa gtaagcggtt ttgtacgctt tactgacatg
    16501 atcgacggcc agaccattac tcgtcagacc gacgaactga ccggtctgtc ttcgctggtg
    16561 gttctggatt ccgcagaacg taccgcaggt ggtaaagatc tgcgtccggc actgaaaatc
    16621 gttgatgctc agggtaacga cgttctgatc ccaggtaccg atatgcctgc gcagtacttc
    16681 ctgccgggta aagcgattgt tcagctggaa gatggcgtac agatcagctc tggtgacacc
    16741 ttggcgcgta ttccgcagga atccggcggt accaaggaca tcaccggtgg tctgccgcgc
    16801 gttgcggacc tgttcgaagc tcgtcgtccg aaagagccgg caatcctggc tgaaatcagc
    16861 ggtatcgttt ccttcggtaa agaaaccaaa ggtaaacgtc gtctggttat caccccggta
    16921 gacggtagcg atccgtacga agagatgatt ccgaaatggc gtcagctcaa cgtgttcgaa
    16981 ggtgaacgtg tagaacgtgg tgacgtaatt tccgacggtc cggaagcgcc gcacgacatt
    17041 ctgcgtctgc gtggtgttca tgctgtgact cgttacatcg ttaacgaagt acaggacgta
    17101 taccgtctgc agggcgtaaa gattaacgat aaacacatcg aagttatcgt tcgtcagatg
    17161 ctgcgtaaag ctaccatcgt taacgcgggc agctccgact tcctggaagg cgaacaggtt
    17221 gaatactctc gcgtcaagat cgcaaaccgc gaactggaag cgaacggcaa agtgggtgca
    17281 acttactccc gcgatctgct gggtatcacc aaagcgtctc tggcaaccga gtccttcatc
    17341 tccgcggcat cgttccagga gaccactcgc gtgctgaccg aagcagccgt tgcgggcaaa
    17401 cgcgacgaac tgcgcggcct gaaagagaac gttatcgtgg gtcgtctgat cccggcaggt
    17461 accggttacg cgtaccacca ggatcgtatg cgtcgccgtg ctgcgggtga agctccggct
    17521 gcaccgcagg tgactgcaga agacgcatct gccagcctgg cagaactgct gaacgcaggt
    17581 ctgggcggtt ctgataacga gtaatcgtta atccgcaaat aacgtaaaaa cccgcttcgg
    17641 cgggtttttt tatgggtgtt attcagaggg caaattcttc acggaaaata ttaagaaagg
    17701 cagggcgaaa cgaggaagaa gcttttcgcc ctggacaaca ttcctgctga cggcactacc
    17761 ataaaaaaac acaacgcttt caacaacctt gcgtttttca tcttccgtag cggctcggaa
    17821 taaatttacg ttcttacatt ccgtctcata gtctttgcga ggcgcgtccc agatagctgt
    17881 cccagtcttt ccataccggc tgcaaaccct gagcggttaa cacggcagca accgcttccg
    17941 gtctgcgatc gtcgtgcggt gagaactgtt ccaactcggg gtgattatcg gcatagccac
    18001 ctggctgcgt tttcgagaag gcgctgacgt tattgatcgc cagcggaata acgcgatcgc
    18061 gaaaccacgg tgattcccgc gtggagagtg acagttcaat ctccggtgca agcagtcgga
    18121 aggcgcagat ggtttgcact aactggcgtt catccataat cgacgcaggc tcaatgccgc
    18181 cagtacacgg gcgcaggcgc ggaaaggaga cagagtaacg gctttgccag taatgctgtt
    18241 gcagccatag caaatgttcg gcaaccatat agcagtcaac gcgccagttg tcggaaaggc
    18301 caattagcgc gccgaggcct atcttatcaa tccccgcacg ccccagccga tccggcgttt
    18361 ccagccgcca gaagaagtcc tgttttttgc ctttcaaatg atggcgggta taagtcgcct
    18421 cgtgatatgt ctcctgataa accatcaccc catccagacc aagttgcttt aactcggcgt
    18481 attccgtctc cgccagcggt tgcacttcca tttgtagtga agagaactgt tcacgcaggg
    18541 cggggagatg acgacgaaag taatccatcc ccactttcgc ctgatgttca ccagtgacta
    18601 atagcagatg ttcaaagccc atctcccgta tagcggcgct ttccctggca atatccgctt
    18661 catccagcgt tttgcgcttg atgcgattac tcatggaaaa tccgcagtac gtgcagtcgt
    18721 tagcgcaaag attggaaaga taaagcggga cgtagaaact aactgtgttg ccaaatcgct
    18781 gacgggtcag acgctgcgcc tgttgggcca gttgttccag atagccactg gcggcaggcg
    18841 ataacagcgc catcatgtcg tcgcgggtga gttgcgaggc atttagcgcc cgctctacgt
    18901 cagcagccgt tttgccgttg atacgcaggc ggatgtcgtc ccagtccagt tgtcgccagc
    18961 gattgctgaa ggttttcatg ccgatgcctc cagaaatccg gtcagcgggc tggtggcatg
    19021 agcaaaataa ctgcggctgc ccggtccgga ctgacgtgcc agcaggccta cttctaccgc
    19081 cagacgaaat gccttcgcca tgttgacggg atcgtccgcg acggcaatcg ccgtattcac
    19141 taacaccgcg tcggccccca tttccagcgc ctgcgtggca tggctgggaa cgccgatgcc
    19201 agcatcgaca accaccggaa ctgtggcctg ctggataata atctccagca tggcacgggt
    19261 ttccagtccc tgattcgagc caatcggcgc gccgagcggc atcaccgctg cacagccgac
    19321 ttcttccaga cgtttacaca ataccggatc ggccccgcag taaggcagca cgacaaatcc
    19381 ctgttgtacc agcgtttcgg cggctttcag ggtttcgatg ggatcgggca acagccagcg
    19441 ggcgtcaggg tgaatctcca attttagcca gtttgtgcct aacgcttcac gtgccagatg
    19501 ggcggcgaaa atggcttctt ccgctgtttt cgccccggaa gtattcggca gcagggccac
    19561 acccgccgcg ataagcggtt cgaggatagc gtcgttgtgc tggcgcaagt cgacacgttt
    19621 catcgccagt gtcaccagct ggctgccgga agcgcggatc gcctccacca tcagttgtga
    19681 agaagcgaat ttccctgtgc cggtaaacag atgtgaatca aacgttttgt ccgcaatacg
    19741 taacatctca accccctgca ataacctgaa aaagcaggat ctggtcgcca tcctgcacga
    19801 tatgttgcgt ccactgctca cgcgggacga tttgctgatt aatcgccaga gccgcgcccg
    19861 cttgtcgttg gtcgagttgc tccagtagtt cgtgaacagt ttgcccggcg gcgcactgca
    19921 tcggttgatc gttaaacagg atctgcattg cttcctccgc ataccgggca accactggcg
    19981 cggcgcaacg ccaggctgcg ccactggctc gacttaccgt cgaacagtcg gagttctccc
    20041 gcaggcgtct ctataccgct taataactta atggcttcca gtgcctgcaa agtgcccatc
    20101 accccgacca ccgggccaac cacgcccgcc gtgcggcagt tgcgttctgg ctgctggtta
    20161 tctggccaca ggcagcggta acacccctgc tcccagggcg gtgtcagtac catcaactga
    20221 ccgccaaatc cgaccgcgct ggcggtgata agcggcgtgt tgagtgccac gcaggcggca
    20281 ttaatctcct ggcgagtcgc catattgtcg gtacagtcga gcaccacatc ggcttgtgca
    20341 accgcatctt tcaacgcctc acccgttaac cgttgttgta atgctgtcag ttgaatgtcg
    20401 ggattcaact gtgtcagtcg ctgttggctg acctgcgatt tcgggctgtt gatatcttca
    20461 gtggtaaaga ggatttgtcg ttgcagattg cttaaatgca catcgtcgtc atctgccagt
    20521 accagcgtcc cgacgccagc ccctgccagg tatagcgcag caggtgtacc cagcccgcca
    20581 agaccaataa tcagcacctg gctgtcgagc agtttttgct gcccgtcaag agcgatatcg
    20641 tcgagcagaa tttggcggct ataacgcata aagtcacggt cattcatcgc caactcctgc
    20701 aatttccagc aactgtgccg ttgccaaacg ccagtctgcg gcttgagtaa tggcgctgac
    20761 gacggcgata ctgccgacac ccgttgctat caccgcaggc gcgcgtgcca gactgatacc
    20821 gccaatcgcc acggtgggat aatccgccag gcgctcaaca tgccgtgcca actgttccag
    20881 cccctgcggt gcagaaggca tctgtttggt ttgcgtcggg aacacatgtc ccagcgcgat
    20941 ataagagggg cgtgctgcca gcgcgacgtc gatttccata tcgtcatgtg tcgaaacgcc
    21001 cagccgcagg cctgccgcgc ggatggcatt gagatcggtg gcttgcaaat cttcctgccc
    21061 caaatggacg ccatacgcct gatgcttgat cgcgagccgc cagtaatcgt tgataaacaa
    21121 tcgcgcgtta tagcggcggc ccagcgcaat tgccgccacg acatcggctt ccacctcttc
    21181 atcgcgccga tctttgatgc gtagctggag agtacgtacg cctgcatcca acagacgttc
    21241 gatccactgt acgctgtcca ccaccgggta cagtcctaaa cgaaaaggta caggaggaaa
    21301 atcaggctga tacatcacgc ttcctcctta tgcaggtaga tttctccgcc tctggcacgg
    21361 aagttctccg acatatccgc cattcccact tcaatggttt gcgcggcggc gtaatcgcgt
    21421 acttcctggc tgattttcat cgagcagaat tttggcccac acatggagca aaaatgggcg
    21481 actttgccgg actcttgggg cagggtttca tcgtgataag cgcgggcggt aaacgggtcg
    21541 agggccagat taaactggtc ttcccagcga aattcgaagc gggctttcga catggcgtta
    21601 tcgcgaattt gcgcgcccgg atgccctttt gccaggtcag cagcgtgggc ggcaatctta
    21661 taagtgataa gcccctgctt aacatcttct ttattgggca gacccagatg ctcttttggc
    21721 gttacgtaac agagcatcgc gcagccaaac cagccaatca tcgccgcacc aatccccgac
    21781 gtgaagtggt catagcccgg tgcaatatcg gtagttagcg gccccagagt gtaaaacggc
    21841 gcttcgtggc agtgctctaa ctcctcggtc atattgcggc ggatcatctg catcggcaca
    21901 tggcctgggc cttcaatcat cacctgtacg tcatattccc aggcgatttt ggtcagttcg
    21961 cccagcgtat gcaattcggc aaactgcgct tcatcgttgg cgtcctgaat agaaccgggg
    22021 cgcagaccgt cgcccagcga cagcgaaacg tcataagcgg cacagatttc acaaatttcg
    22081 cggaagtgtt gatagaggaa attttcctga tgatgggaga ggcaccattt cgccataatc
    22141 gaaccgccgc gagagacgat accggtcagg cgtttcgcgg tcatcggcac atagcgcagc
    22201 agtacgcccg catggatagt gaagtaatcc acaccttgct cggcctgttc cagcagcgtg
    22261 tcgcggaacg cttcccaggt aagatcttcg gcgatcccgt taaccttctc cagcgcctgg
    22321 tagatcggca ctgtaccgat cggcaccgga ctgttacgca aaatccactc gcgggtttcg
    22381 tgaatatagc gaccggtgga gagatccatc accgtatccg ctccccagcg cgtggaccat
    22441 accagctttt ccacttcttc ttcgatggaa gaggtgaccg ccgagttgcc gatattggcg
    22501 ttaactttta ccaggaagtt acggccaata atcatcggct ccgattccgg atgattaatg
    22561 ttggccggga taatcgcacg tccggcagca acttcatcac ggacaaattc cgcagtgata
    22621 ttttccggca gatgtgcgcc aaagctcatt cccggatgct ggtggcgtaa aacctcgcta
    22681 cggatgcgct cgcggcccat attctcgcgg atggcgatga attccatgtc cggcgtgatg
    22741 atgccctggc gggcgtagtg cagttgggtg acacggcgtc ctgttttggc gcgttttggc
    22801 gttaataagc cgctaaaacg tagctcgtcg agaccatcat ctgccagccg cgttctggtg
    22861 taatcggaac tgcgcacggt aagttcttcg gtatcggcgc gcgcatcgat ccacggctgg
    22921 cgtagttttg ccagcccttg ctgcacgtta atggcgatct gcggatcacc atacgggccg
    22981 gaggtgtcgt agaccggaat cgcttcgttt tcttcgtact gcggctgttc tttgctaccg
    23041 ccaattagcg tcgggctaag ctggatctca cgcatcggca cgcgcacgcc ggggtgtgtg
    23101 ccagtgatat aaatgcgttt tgagttggga aaggcggtgc cttccagggt gtcgataaaa
    23161 tgttgggccc gggcgcgttg ttcgcggcgg gtcagttttg ttgcagacat agctcattcc
    23221 aaaaagttaa ggacgtggct tgtcagacga cggatgaagc aagagacgat cgccgcaaag
    23281 gcgatgcgat agcagattaa ctcttgttcc cttcgcaggt attagcctga tcaggttccg
    23341 cggatcccga ataaacggtc tcagccagtt aaggcactcc gacaagaaat tagcccccac
    23401 aaacggggcg ttgaatgtaa attacgcgtt aacagcgcag aactcaagca ggatgtttga
    23461 cgcgggcggc gtcaagcacc agcagaatca gcttatcttc cagcacaaag cgcgcttcca
    23521 gcgcctcgcc gatgtcagat aaaacctgtt gaaactcaag gtaattatca tgatcgatgg
    23581 cggtttccag gctggaatcg tagtaatcca taatctgttg ggtattggct tcgagttgcg
    23641 gccaaatctt cgcggcgcgc gcgagttgcc cgttgccttc cagcttatga agaatgcgct
    23701 cataaatact gaaatgtccg gcagacaagt aatcgaccag gctctgacaa aaatcatcaa
    23761 gggctttttc gtttagcctc atgtacgatt ctttgccagg cttaatgcca accagattgt
    23821 agtaagccac gagcagatgc ttacgtacat gtagccagcg atcaaccagt ttgttacttc
    23881 ctctgacgcg ctccgtcagg ttatcgagct ggttaagcat gattgactcc gcaagtttgt
    23941 attcaaaaac tgctcagtga gaaatgtaaa aaccatgtta aacatgccag tgatgcaaag
    24001 gtagtgcaag agctatggat cgtataattg aaaaattaga tcacggctgg tgggtcgtca
    24061 gccatgaaca aaaattatgg ttgccgaagg gagaattgcc atatggcgaa gcggcaaatt
    24121 tcgatcttgt gggtcagcgc gcactacaaa tcggcgaatg gcagggggaa cctgtttggt
    24181 taatacaaca gcagcggcgt cacgatatgg ggtcggtacg tcaggtcatt gatctcgatg
    24241 ttgggctgtt tcaactggcc ggacgtggcg tgcaactggc ggagttttac cgatcgcata
    24301 aatactgtgg ttactgcggg catgaaatgt atccgagcaa aaccgaatgg gcgatgctgt
    24361 gtagccattg ccgtgagcgt tactacccgc aaatcgcccc ctgcattatt gttgccatcc
    24421 gtcgcgatga ttcgctcctc ctcgcccagc atacccgcca tcgtaacggt gtccatacag
    24481 tacttgccgg attcgtcgaa gtgggcgaaa ccctcgagca ggcagtcgcg cgggaagtga
    24541 tggaagagag cggaattaaa gttaaaaact tgcgttacgt gacttctcag ccgtggccgt
    24601 ttcctcagtc tttaatgacc gcgtttatgg cggaatatga cagcggcgac atcgtgatcg
    24661 acccgaaaga attgctcgag gcgaactggt atcgctatga cgatttgccg ttactcccgc
    24721 cgcccggcac cgtagcgcgc cgtctgatag aagatactgt ggcgatgtgt cgggcagagt
    24781 atgagtgatg atacactgac cgcctgacgc actaaggaac agccaaaatg accgaactta
    24841 aaaacgatcg ttatctgcgg gcgctgctgc gccagcccgt tgatgtcact ccagtatgga
    24901 tgatgcgcca ggcgggtcgc tatctaccgg aatataaagc cacgcgcgcc caggcgggcg
    24961 attttatgtc gctgtgcaaa aacgccgagc tggcgtgcga agtgactttg caaccgctgc
    25021 gtcgctaccc gctggatgcg gcgatcctct tttccgatat cctcaccgtg ccggacgcga
    25081 tggggttagg gctctatttt gaagccggag aaggtccgcg ttttacctcg ccagtcacct
    25141 gcaaagccga cgtcgataaa ctgccaattc cggacccgga agatgagctg ggttacgtga
    25201 tgaacgcggt gcgtaccatt cgtcacgaac tgaaaggcga agtgccgctg attggttttt
    25261 ccggcagccc gtggacgctg gcaacctaca tggtggaagg cggcagcagc aaagccttca
    25321 ccgtgatcaa aaaaatgatg tatgccgatc cgcaggcgct gcacgctctg ctcgataaac
    25381 tggcgaaaag cgtcactttg tatctgaatg cgcagattaa agccggtgct caggcagtga
    25441 tgattttcga cacctggggc ggcgtgctta ccgggcgcga ttatcaacag ttctcgctct
    25501 attacatgca taaaattgtt gatggtttac tgcgtgaaaa cgacggtcgc cgcgtgccag
    25561 tcacgctgtt taccaaaggc ggcggacagt ggctggaagc catggctgaa accggttgcg
    25621 atgcgctggg cctcgactgg acaacggaca tcgccgatgc gcgccgccgt gtgggcaata
    25681 aagtcgcgtt gcagggtaat atggatccgt cgatgctgta cgcgccgcct gcccgcattg
    25741 aagaagaagt agcgactata cttgcaggtt tcggtcacgg cgaaggtcat gtctttaacc
    25801 ttggtcacgg cattcatcag gatgtgccgc cagaacatgc tggcgtgttc gtggaggcag
    25861 tgcatcgact gtctgaacag tatcaccgct aagagcctga tccatcaggc tattttattt
    25921 gccattttga ggccgggcag tgctcaaaat cctcacgtac tacgtgtacg ctccggtttt
    25981 tccgcgctgt ccggcctcaa actgccttca acaattacgc ctgttgaacc aggctctaag
    26041 gagcgattat ggatctcgcg tcattacgcg ctcaacaaat cgaactggct tcttctgtga
    26101 tctgcgagga tcgactcgat aaagatccac cggatctgat cgccggagcc gatgtcgggt
    26161 ttgagcaggg cggagaagtg acgcgagcgg cgatggtgct gctgaaatat ccctcgcttg
    26221 agctggtgga gtataaagtt gcccgcatcg ccaccaccat gccttacatt ccaggttttc
    26281 tttccttccg cgaatatcct gcgctgctgg cagcgtggga gatgctgtcg caaaagccgg
    26341 atttagtgtt tgtcgatggt catgggatct cgcatcctcg ccgtcttggc gtcgccagcc
    26401 attttggctt attggtggat gtgccgacca ttggcgtggc gaaaaaacgg ctctgcggta
    26461 aattcgaacc gctctccagc aaaccgggcg cgctggcccc actgatggat aaaggcgagc
    26521 agctggcctg ggtctggcgc agcaaagcgc gctgtaaccc gttgtttatc gctaccggcc
    26581 atcgggtcag cgtggacagc gcgctggcgt gggtacaacg ctgcatgaaa ggctatcgtc
    26641 tgccggagcc aacgcgctgg gcggacgcgg tggcctcgga acgtccggcg ttcgtgcgct
    26701 atacagcaaa tcagccctaa ttcaggtaaa ctgcggataa tttccgtatt tgagaactca
    26761 acatgttaca aaacccaatt catctgcgtc tggagcgcct ggaaagctgg cagcatgtca
    26821 ctttcatggc ttgcttatgc gaacgcatgt accccaatta cgccatgttc tgccagcaaa
    26881 ccggttttgg tgatgggcaa atttaccgtc gtattctcga tctcatctgg gaaacgctga
    26941 ccgttaaaga cgcaaaagta aatttcgaca gccaactgga gaaatttgaa gaagcgattc
    27001 cttcagccga cgatttcgat ctgtacggcg tttatccggc aatcgatgcc tgcgtggcgt
    27061 taagtgaact ggtccattcg cgtttgagtg gtgaaacgct cgaacacgcg gtggaagtga
    27121 gtaagacctc catcacgacc gttgcgatgc tggaaatgac tcaggctggt cgcgaaatgg
    27181 gcgatgaaga gcttaaagaa aacccagctg tagagcaaga atgggacatt cagtgggaaa
    27241 tattccgact tttagctgag tgcgaagaac gcgatatcga gctgataaaa ggccttaggg
    27301 cagacctgcg tgagtcgggt gagagcaata ttggtataat ttttcagcaa taagaccaga
    27361 aaacgtgatt taacgcctga tttgtcgtac ctggagtctt ccctttcgcc ccccgtctgg
    27421 tctacatttg gggggcgaaa aaaagtggct atcggtgcgt gtatgcagga gagtgctatt
    27481 ctggcatttc cgtcgcactc gatgcttagc aagcgataaa cacattgtaa ggataactta
    27541 tgaacaagac tcaactgatt gatgtaattg cagagaaagc agaactgtcc aaaacccagg
    27601 ctaaagctgc tctggagtcc actctggctg caattactga gtctctgaaa gaaggcgatg
    27661 ctgtacaact ggttggtttc ggtaccttca aagtgaacca tcgcgctgag cgtactggcc
    27721 gcaacccgca gaccggtaaa gaaatcaaaa ttgccgcagc taacgtaccg gcatttgttt
    27781 ctggcaaggc actgaaagac gcagttaagt aagattgcgt ggcagtgaac agttttaacg
    27841 aaggggtggt ttcacccctt ttgtctttct ggcgtcgatc attgatgctg gcaggtgcgc
    27901 tgtttctcac tgcctgtagc cacaactctt cacttcctcc ctttaccgcc agtggatttg
    27961 ctgaagacca gggcgcggta cgcatctggc gaaaagacag cggcgataat gtgcatctgc
    28021 ttgccgtgtt tagcccgtgg cgcagtggcg ataccacgac gcgagagtat cgctggcagg
    28081 gcgataacct cacgctcatc aatatcaatg tttacagcaa accgccggtg aacattcgcg
    28141 cgcgttttga cgatcgcggc gatctgagct ttatgcaacg tgaatccgat ggggaaaagc
    28201 agcagctttc taacgaccaa atcgatttat accgttatcg tgctgatcag atccgccaga
    28261 ttagcgatgc cttacgtcag gggagagtcg tgctgcgcca ggggcgctgg catgcgatgg
    28321 aacagaccgt gaccacctgc gaagggcaaa ccattaaacc tgatttagat tcgcaggcga
    28381 tagcgcatat cgagcgccgc cagagccgct cttctgttga tgtcagcgtg gcatggctgg
    28441 aagcgcccga aggttcgcaa ttactgttag tggcaaactc tgatttctgt cgctggcaac
    28501 ccaacgagaa aacgttctga tttaccagtg gcccataccc atatgaccgc caccaccgca
    28561 gccgccgtag cccatgccca ttccggctcc gcgcggaata cccgcttcag ccatcgcgat
    28621 atctcgtttc acccgtaact catctaacga ctgacgcaaa ttctccatct ctttggcgac
    28681 cgcgttaatt ttgctgctat ccggtgggtt cgcggctaac agggcattgt attcataacg
    28741 cttcgtcacc agttgctgtt gcagtgcgct gctttgagcg taaaagtcat tatggatttt
    28801 ctgccacgct gtctgttgtt cgctggtcaa aggcgcggca ttttgctgcc acataccgtg
    28861 tccgccgtga gcaaatgcag atgtcgatcc catcgccatt gctgaaagcg ccatcattac
    28921 cagggcaatt ttcgtgttcc gtttcatggt taatcctcca gtggttgtct tacttcgggt
    28981 attgcatctt ccgtgccaac gatgaaacgc tgatatgacg ggtaatctgg catgataaac
    29041 gagtaaaaat gactcgcctg ctgcgggtag cgagtcattt ttactcattg aaacttaagc
    29101 ctttgtgtta cagcgcaggg taagcgctga taaaagatgg catgatttct gctgtcagaa
    29161 agggatgagc aggcaaagaa gaagatgcgt tttatgcaac gttctaaaga ctccttagct
    29221 aaatggttaa gcgcgatcct ccccgtggtc attgttgggc tggtagggtt gtttgcggtg
    29281 acggtgattc gcgattatgg gcgcgagact gccgccgcca gacaaacgct gctggaaaaa
    29341 ggcagtgtac ttattcgcgc tcttgaatcc ggctcgcgcg tcggcatggg gatgcgcatg
    29401 catcatgcgc agcagcaggc cttactggaa gaaatggccg ggcagcctgg agtacgttgg
    29461 tttgcggtca cggatgaaca aggaacaatc gtgatgcata gcaactccgg catggtggga
    29521 aaacagcttt attcccctca ggaaatgcag cagttacatc cgggagatga agaagcgtgg
    29581 cggcggatcg atagcgcaga cggcgagcct gttctggaaa tttatcgcca gtttcaaccg
    29641 atgtttgctg ctggaatgta ccggatgcgc catatgcagc agtatgccgc gacaccacaa
    29701 gcaattttca tcgctttcga cgccagtaat attgtgagtg ccgaagatcg tgagcagaga
    29761 aacaccctga ttattctctt cgccctggcg acgctcttgc tggcaagcgt attgtcattc
    29821 ttctggtatc gccgctatct gcgctcgcgc cagcttctac aagatgaaat gaagcgcaaa
    29881 gagaagctgg tggcgctggg gcatcttgcg gcaggcgttg cccacgaaat ccgtaaccca
    29941 ctttcctcga ttaaaggact ggcgaaatac tttgccgagc gcgcgcctgc agggggagaa
    30001 gcgcatcaac tggcgcaggt gatggcgaaa gaggccgacc gtttaaaccg cgtggtaagc
    30061 gagttgctgg aactggttaa gccaacgcat ctggctttgc aggcggtgga tctcaacacg
    30121 ctgattaacc actcattaca gctggtaagc caggatgcaa acagccggga gatccagtta
    30181 cgctttaccg ccaacgacac attaccggaa attcaggccg atccggacag gctgactcag
    30241 gtcctgttga atctctatct caatgctatt caggcgattg gtcagcatgg cgtgattagc
    30301 gtgacggcca gcgaaagcgg cgcgggcgtg aaaatcagcg ttaccgacag cggtaaggga
    30361 attgcggcag atcagcttga agccatcttc actccgtact tcaccaccaa agccgaaggc
    30421 accggattgg ggctggcggt cgtgcataat attgttgaac aacacggtgg tacaattcag
    30481 gtcgcaagcc aggagggaaa aggctcaacg ttcaccctct ggcttccggt caatattacg
    30541 cgtaaggacc cacaaggatg acgcacgata atatcgatat tctggtggtg gatgatgaca
    30601 ttagccactg cactattttg caggctttac tgcgcggctg gagctataac gtcgcgctgg
    30661 cgaacagcgg gcgacaggcg ctggagcagg tgcgggaaca ggtttttgat cttgtgcttt
    30721 gcgatgtgcg aatggcggag atggacggca tcgccacgct gaaagagatc aaagcgttaa
    30781 acccggcaat tccggtgctg attatgactg cgtactccag cgtcgagacg gcggtagagg
    30841 cactgaaaac tggggcgcag gattatctca tcaagccgct ggatttcgat aacctacagg
    30901 cgacgctgga aaaggcgctc gcgcatacgc acagtattga tgctgaaaca cctgcggtga
    30961 ctgccagcca gttcggtatg gtcggtaaaa gcccggcgat gcaacacctg ctcagtgaaa
    31021 tcgccctcgt cgcgccatcg gaagccacgg tgctgatcca cggcgattcc ggcaccggta
    31081 aagagctggt cgccagggcg attcacgcca gtagcgcacg tagcgaaaaa ccgctggtaa
    31141 cgctcaactg tgcggcactc aacgaatcct tgctggaatc tgaattgttc ggtcacgaaa
    31201 aaggggcgtt tactggagcc gataaacgcc gggaagggcg ctttgttgag gcggacggcg
    31261 gcacgctgtt tctcgatgaa attggcgata tctcgccgat gatgcaggtg cgtctgctgc
    31321 gtgcgattca ggagcgcgaa gttcagcgtg tcggtagcaa ccagactatc tcggttgatg
    31381 tccggctgat tgcggcgacc catcgcgatc ttgccgcaga ggtgaatgcc gggcgttttc
    31441 gccaggatct ctactatcgc ctgaatgtgg tggcgattga agtaccatcg ctgcggcaac
    31501 ggcgggaaga tatccctctg ctggctggcc attttcttca gcgctttgcc gagcgtaatc
    31561 gcaaggcggt aaaaggcttt acgccacagg cgatggattt gctgatccac tacgactggc
    31621 cgggaaatat tcgtgaactg gaaaacgcgg tggaacgggc ggtggtgctg ctgaccgggg
    31681 aatatatttc cgaacgcgag ctgccgctgg cgattgccag tacgccgatc ccgctggggc
    31741 aaagtcagga tattcagccg ctggtggaag tggaaaaaga ggtgattctg gcggcgctgg
    31801 agaaaacggg cggcaacaaa accgaagccg cccgtcagtt agggatcacg cgcaaaacgc
    31861 tattggcaaa actgtcgcgt tagttctgct cgcgttcgat ggcgcgccaa ccgatatctt
    31921 tccggcagaa gcaatcatcc cagtggatat cggtcattaa ggcgtaggcg cgtttctgcg
    31981 cttctgccac ggtatgaccc agcgcggtga cgcacagtac gcgcccgccg ctggttacca
    32041 cctgctcgtc atccgccagt tttgtgcccg cgtggaacac tttgccgtca gccacttctt
    32101 ccagcggcag gccgtggatc acatcgccgg tgcggtaatc gcccggatat ccacccgcag
    32161 ccatcaccac gctgagagaa gcacgttcat cccactctga cgttttctcg tccagtttgc
    32221 cttcacaggc cgccaggcag agctcaacca gatcggactt catgcgcagc ataatcggct
    32281 gggtttccgg atcgccaaag cggcagttaa attcaataac cttcggattg ccctgtttgt
    32341 cgatcatcag gcccgcatag agaaaaccgg tgtaggtgtt gccttccgcc gccatgcctt
    32401 tcacggttgg ccagatgata cgttccatgg tgcgctgatg aacttcatcg gttactaccg
    32461 gcgcggggga gtaagcgccc atcccgccgg tgtttggtcc gttatcttta tcgcctacgc
    32521 gtttgtgatc ctggctggta gccatcggca gcacatgctc gccgtccacc atcacgataa
    32581 agctcgcttc ttcgccatcg aggaactctt cgataacgat gcgatgaccc gcgtcgccaa
    32641 aagcgttgcc cgccagcata tcgtgaacag ccgcttccgc ttcttccagc gtcatcgcca
    32701 cgataacgcc tttcccggca gccagaccgt ccgctttaat gacgattggc gcgcctttct
    32761 cacgcagata caccagcgca ggttctacct cggtgaagtt ctggtattcc gccgtaggga
    32821 tcttatggcg ggccaggaaa tctttggtaa acgcttttga gccttccagt tgggccgcac
    32881 ctgcggttgg gccgaagatt ttcagcccgg cggcgcggaa ggtatcgacc acgcctttca
    32941 ccagcggcgc ttccgggccg acgatggtca gatcaatctt ttcgttttgt gcgaaatcca
    33001 acagcgccgg gatatcggtc acgccgatag caacgttttg cagcgcgggt tccagcgcag
    33061 tgcctgcatt acccggagca acaaaaacag tctcaaccag cggcgactgg gccgctttcc
    33121 aggccagcgc gtgctcgcgc ccgccgttac caatcactaa tactttcatc tattgctcca
    33181 ttaatggcgg aagtggcgca tgtcggtgaa gagcatcgca ataccgtgct cgtcggcggc
    33241 ggcaatcact tcgtcatcac ggatggaacc gccaggctgg attacgcagg tcacgcccgc
    33301 agcggcggcg gcatcaatac cgtcgcggaa cgggaagaac gcgtcagaag ccatcgagga
    33361 acctttcact tccaggcctt catcggcagc tttaataccg gcgattttcg cggagtacac
    33421 gcggctcatc tggcccgcgc caataccgat ggtcatattg tttttggcat agacgatagc
    33481 attggatttc acgaatttcg ccactttcca gcagaacagc gcatcacgca gttcctgttc
    33541 ggtcggctga cgttgggtga cgacgcgcag ttcttccgca ccgaccatcc ccaggtcacg
    33601 atcctgaacc agcagaccgc cgttcacgcg tttgaaatca agacccggaa cacgctcgcc
    33661 ccactgaccg caggtcagaa cgcgtacgtt ctgcttggcg gcggtgattt tcagggcttc
    33721 ttcgctggcg gaaggcgcaa taatcacttc gacaaactga cgagaaatga tggcctgtgc
    33781 ggtttccgca tccagctcgc ggttaaaggc aatgatgccg ccgaatgcgg aggttgggtc
    33841 ggttttgtac gcgcgatcgt aagcatcaag aatggaattg ccgatagcta caccgcaagg
    33901 gttggcgtgc ttcacaatca cacatgccgg ctcggcgaac tctttcacgc actccagcgc
    33961 cgcatcggta tcggcgatgt tgttataaga gagggcttta ccctgaacct gggttgcggt
    34021 agcaacggag gcttctttca cattctcttc tatatagaag gcagcctgct ggtggctgtt
    34081 ctcgccgtaa cgcatatcct gcttcttaat gaagttcagg ttcagcgtgc gtgggaagcg
    34141 accggcggct tctttgcttt caccgtggta cgccggaacc atgctgccga agtagttggc
    34201 aatcatgctg tcgtaggcgg cagtgtgttc gaaggctttg atggcgaggt cgaaacgggt
    34261 tgcaagcgtc agcgatcctt cgttgtcatc catctcttta ataatggcgt cgtagtcgct
    34321 gctcttcacc acgattgcga catctttatg gttcttagcg gcggagcgca ccatggtcgg
    34381 gccgccgata tcgatgttct caaccgcatc ttccagcgag cagccttcac gagcaacagt
    34441 ctgggcgaac ggatacaggt taacaaccac catatcgata ggctggatct gatgttcttc
    34501 cataatggca tcgtcctggc cgcgacggcc cagaatgcca ccatgtactt tcggatgcag
    34561 ggtcttcacg cgtccatcca tcatctccgg gaaaccggtg taatcggaaa cttcggttac
    34621 cggcagacct ttttctgcta acagacgggc agtgccccct gtagacagca gctccacacc
    34681 gcgtgcggaa agtgcctggg cgaattcgac gataccggct ttgtcagaaa cactgagcag
    34741 agcgcggcgg actggacgac gttgttgcat ggtaaatccc ctgggtttga ctattacaga
    34801 gagcgttagc tgaatttttc gcgaaaaact cagctaacgc ccctaacggg gcatccttat
    34861 ttttcgcccg cattgtaacg aaaacgtttg cgcaacgctc gcgaattttt ctctttcaat
    34921 ggtgatcaca attttgacag tggttaccgt gggaaaaata cagaaattac attgatgatt
    34981 gtggataact ctgtgcgtaa aaaggtataa agcggggttt tgctggggaa tgcagcagtc
    35041 agtcattttt ctgcaatttt tctattgcgg cctgcggaga actccctata atgcgccttc
    35101 atcgacacgg cggatgtgaa tcacttcaca caaacagccg gttcggttga agaga
//
LOCUS       DAEEUE010000034        34939 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.034, whole genome shotgun sequence.
ACCESSION   DAEEUE010000034 DAEEUE010000000
VERSION     DAEEUE010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34939
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.034"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..2262
                     /locus_tag="KLK84_001800"
     CDS             <1..2262
                     /locus_tag="KLK84_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HBE2009153.1"
                     /translation="DAKSLYLNPDKNTVNAFIHQQDINVLAQDDYQSLSIQGNGALGI
                     TENSYIGAHWNFNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNAQGG
                     NFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLGSFY
                     LNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQAGKT
                     TSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNLAIS
                     ASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYESISA
                     TVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSASRA
                     IVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTDYRY
                     SEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYATVS
                     DSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDEESQ
                     DLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAGNRE
                     LMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLEING
                     SEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQPKL
                     RGNVQTAQREVAP"
     gene            2259..3348
                     /locus_tag="KLK84_001801"
                     /pseudo
     CDS             2259..3348
                     /locus_tag="KLK84_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
     gene            complement(3436..4734)
                     /gene="exuT"
                     /locus_tag="KLK84_001802"
     CDS             complement(3436..4734)
                     /gene="exuT"
                     /locus_tag="KLK84_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HBE2009154.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5218..6630
                     /gene="uxaC"
                     /locus_tag="KLK84_001803"
     CDS             5218..6630
                     /gene="uxaC"
                     /locus_tag="KLK84_001803"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBE2009155.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6645..8132
                     /gene="uxaA"
                     /locus_tag="KLK84_001804"
     CDS             6645..8132
                     /gene="uxaA"
                     /locus_tag="KLK84_001804"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBE2009156.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8215..8766
                     /locus_tag="KLK84_001805"
     CDS             8215..8766
                     /locus_tag="KLK84_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HBE2009157.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8771..10015)
                     /gene="sstT"
                     /locus_tag="KLK84_001806"
     CDS             complement(8771..10015)
                     /gene="sstT"
                     /locus_tag="KLK84_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBE2009158.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10611..11576)
                     /gene="alx"
                     /locus_tag="KLK84_001807"
     CDS             complement(10611..11576)
                     /gene="alx"
                     /locus_tag="KLK84_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HBE2009159.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11847..12845)
                     /locus_tag="KLK84_001808"
     CDS             complement(11847..12845)
                     /locus_tag="KLK84_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBE2009160.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12924..13616)
                     /locus_tag="KLK84_001809"
     CDS             complement(12924..13616)
                     /locus_tag="KLK84_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HBE2009161.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13689..14192)
                     /locus_tag="KLK84_001810"
     CDS             complement(13689..14192)
                     /locus_tag="KLK84_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBE2009162.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14277..15413
                     /gene="rlmG"
                     /locus_tag="KLK84_001811"
     CDS             14277..15413
                     /gene="rlmG"
                     /locus_tag="KLK84_001811"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HBE2009163.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15698..16012
                     /gene="higB"
                     /locus_tag="KLK84_001812"
     CDS             15698..16012
                     /gene="higB"
                     /locus_tag="KLK84_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HBE2009164.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            16009..16425
                     /gene="higA"
                     /locus_tag="KLK84_001813"
     CDS             16009..16425
                     /gene="higA"
                     /locus_tag="KLK84_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HBE2009165.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16470..18488)
                     /gene="fadH"
                     /locus_tag="KLK84_001814"
     CDS             complement(16470..18488)
                     /gene="fadH"
                     /locus_tag="KLK84_001814"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HBE2009166.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTILLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18714..21065)
                     /gene="ygjK"
                     /locus_tag="KLK84_001815"
     CDS             complement(18714..21065)
                     /gene="ygjK"
                     /locus_tag="KLK84_001815"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBE2009167.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(21082..21501)
                     /locus_tag="KLK84_001816"
     CDS             complement(21082..21501)
                     /locus_tag="KLK84_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009168.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21479..21832)
                     /locus_tag="KLK84_001817"
     CDS             complement(21479..21832)
                     /locus_tag="KLK84_001817"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009169.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(21833..22153)
                     /locus_tag="KLK84_001818"
     CDS             complement(21833..22153)
                     /locus_tag="KLK84_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009170.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(22287..23720)
                     /locus_tag="KLK84_001819"
     CDS             complement(22287..23720)
                     /locus_tag="KLK84_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBE2009171.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23783..24232)
                     /locus_tag="KLK84_001820"
     CDS             complement(23783..24232)
                     /locus_tag="KLK84_001820"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HBE2009172.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24229..27306)
                     /gene="ebgA"
                     /locus_tag="KLK84_001821"
     CDS             complement(24229..27306)
                     /gene="ebgA"
                     /locus_tag="KLK84_001821"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HBE2009173.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLDDYT
                     LHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTRYSE
                     AGHSIASYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSGKPT
                     SCQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSDGEV
                     LIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMGING
                     EYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTALTN
                     RHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSNSWG
                     SEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27489..28472)
                     /gene="ebgR"
                     /locus_tag="KLK84_001822"
     CDS             complement(27489..28472)
                     /gene="ebgR"
                     /locus_tag="KLK84_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HBE2009174.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28690..29021
                     /locus_tag="KLK84_001823"
                     /pseudo
     CDS             28690..29021
                     /locus_tag="KLK84_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(29063..30442)
                     /gene="ygjG"
                     /locus_tag="KLK84_001824"
     CDS             complement(29063..30442)
                     /gene="ygjG"
                     /locus_tag="KLK84_001824"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HBE2009175.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30860..32380
                     /gene="aer"
                     /locus_tag="KLK84_001825"
                     /pseudo
     CDS             30860..32380
                     /gene="aer"
                     /locus_tag="KLK84_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32534..33157)
                     /gene="nfeR"
                     /locus_tag="KLK84_001826"
                     /gene_synonym="yqjI"
     CDS             complement(32534..33157)
                     /gene="nfeR"
                     /locus_tag="KLK84_001826"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HBE2009176.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33434..34179
                     /gene="nfeF"
                     /locus_tag="KLK84_001827"
                     /pseudo
     CDS             33434..34179
                     /gene="nfeF"
                     /locus_tag="KLK84_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(34233..34308)
                     /locus_tag="KLK84_001828"
     tRNA            complement(34233..34308)
                     /locus_tag="KLK84_001828"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34273..34275),aa:Ile,seq:cat)
     gene            34433..34939
                     /gene="mug"
                     /locus_tag="KLK84_001829"
                     /pseudo
     CDS             34433..34939
                     /gene="mug"
                     /locus_tag="KLK84_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8539 a         8974 c         9175 g         8251 t
ORIGIN      
        1 gatgcgaagt cattatattt aaatccagac aaaaatactg ttaacgcttt tatacatcag
       61 caagacatca atgttctggc acaggatgat taccaatcgt tgtctattca gggaaacggt
      121 gcgctgggaa taacagaaaa tagctatatt ggtgcacact ggaatttcaa cggttatgat
      181 gcagatgatg tcagtgacag taatactgac gtcagcgatc tctattatcg ttatgatttt
      241 ttacgtcgtt attatgtgca ggcgggtcgc atggacaacc gcacactatt taatgcacaa
      301 ggcgggaact ttacctttaa ctttctgcca ctcggtgcaa tcgatgggat gcgtatcggg
      361 tcgacactga gctatttaaa ccaagcgcaa agccagcaag gaaccccagt aatggttctg
      421 ctttcgcgca attctcgtgt tgacgcttat cgtaatgagc aacttttggg atcgttttat
      481 ctcaatagtg gttcgcaatt tattgatacc agttcctttc cgccaggcag ctacagcgtg
      541 gcattaaaag tctacgaaaa taaccaactc acccgcaccg agcttgtgcc gtttaccaaa
      601 accggcggtc tgactgacgg aaatgcgcaa tggttcttac aggcaggtaa aactacatca
      661 caggtttctg atgatgaaag ttcagcttat cagctaggag tacgcctgcc attacatccg
      721 caatatgagc tctacgcagg gctggcgaat gccgatgatg tgagtgcttt cgagttaggt
      781 aataactgga cggcagattt aggcagggtg gggaatcttg caatcagcgc cagcgtgttc
      841 cgtaacgatg acggcggcaa aggtgatatg caacaggcca actggagtaa tccgggatgg
      901 ccgacgttgg gcttttatcg gaccaactct gacggcgatg cttgtacaac cgacagcaga
      961 gagagctata acgccttaag ctgttatgaa agtatttccg cgacagtttc actgaatttt
     1021 gtcggctgga atatgatgct gggttatacc tgcacacaaa ataacactga tgatagtttg
     1081 cgttgggata aacagcagag ctttgaaaat aactatcttc gccagacaac agcgcaaagt
     1141 atctctgaaa ctgtacaact tagcgcttcc cgcgctattg tgatgcgtga ctggattttg
     1201 agtacgtcag tcggtgtttt ccatcgtaat gacaacggtg gcgataacga cgacaacggc
     1261 ttgtacttat cgttttcgtt atctgacacg ccaacgatgg atagcaataa caacagccat
     1321 tcaaccaatg tgcctacgga ttatcgttat agcgaacagg atggcgatca aacgtcatgg
     1381 cagttatcgc atacctttta taacgattca ttcagccata aagaacttgg cgtaaccgtc
     1441 ggaggcctga acaccgatac cataaacagc gcggttaacg ggcgttggga tgggcaatac
     1501 ggaaatgtct acgctaccgt atctgatagt tatgaccgta agaatcatga tcatctttcg
     1561 gcctttacgg gcacttatag ctctacgctg gctgtcagtc gctatggcgt taatttggga
     1621 gccagtggta cagacgattt gctgggtgcg gtactggtgg atgtgaaagg cttctctgaa
     1681 caggatgaag agagtcagga tctgcaactc gaagcgcggg tggcaggcag ccgaactttg
     1741 cagcttggtc aaagtgacag tgtgttgttc ccttatcctg gatttcagtc tggttttgtt
     1801 gaggttaacg acagtagcca gggcaatcag caggggacaa caaacatcat taacggtgca
     1861 gggaatcgtg aattaatgtt gttgcctggc aagctgcgct atcgcgaagt gtctgccagc
     1921 tttaactaca actatatcgg tcgtttatta ctgccggcag cggtgaaaaa attcccgatt
     1981 gtggggctga acagcgccat gttactggta gctgaagatg gcggatttac acttgaaatt
     2041 aacggcagcg aaaaagagct ctatctgctt tccgggcagc aattccttaa gtgtccactg
     2101 agtgttgtaa agaaacgcgc cagcattcgt tacagcggag atgttacttg tagtgtggtg
     2161 acttattcac aattaccgga gtcgattcag gttcaggcac agttgaaaca gcctaaatta
     2221 cgtggaaacg ttcagacggc gcaaagggag gttgcaccat gagaaatcga ttgattgcgg
     2281 cgatattggg cttgtttggc acgctcactg gcgttcaggc agctcctgac gtgaccagtg
     2341 aaattacgta tgatttggca tctggtagag cggattatta cttctggaag gatgaagcgt
     2401 cggcaggaaa taatggatat atgtggtatg aatgttcgta tcctgacctc caacaaacct
     2461 gtacagctaa tggaaatata tcgacagtac aaatctattt aactgaacaa cgcagtggga
     2521 tgcgttggcc ggtaaaactc aaaggattta aaacagccat tgtaagtagc gatgaagcgc
     2581 cgctaggatg caaggggggc aaagggcttc agacgaatct taaggattct aatagatctt
     2641 catgtacaga agatggtcaa cattattata tatacgatac aaagtttctt acgctctacc
     2701 ttgagcagac agagatgaag aatttgccga ttggtggcgt ctggaagggg aaagttaaat
     2761 tacattcgaa cagcccggcc caggactatt tcgcaaatat taccctgaat acgctcgacc
     2821 ccaaccatat tgacgtgttc ttcccggagt tcgcccacgc cacgccaagg gtgcagttag
     2881 acttgcatcc aacaggaagc gttaacggca gcaactacgc gcaagatctg accatgttgg
     2941 acatgtgcct gtacgatggt tttaacggta atgccatcag ttatgaaatc atgctcaaag
     3001 atgaagggcg accagctgca gggcgcagag acggttactt ctctaatcta tcgtcaggga
     3061 gggaccacca ccgacgaggg agaacgcatt gattaccggg tcaaaatgta caacccggaa
     3121 accggtgggc aaattgatgt gcgcaataat gaaaatatgg tctggaacag cattaacctg
     3181 aaacgtgtgc gtccggtggt actgcccggt attcgctatg ccgtgatgtg tgtgccgaca
     3241 cccttgactc tggcagtcga taaattcagc gtgatggata aacaggccgg gtactacatg
     3301 ggcaaattgt cagtaatctt tacgccttcc ttgccaacca tcaattaacc ctgcaaaccg
     3361 gacaaacgct tttttctata taaacgacga agccgcctga taaggcggct tcgtcgttat
     3421 aacttacgtt aaacgttaat gttgcggtgc gggatcgtta tgtgtttcct gcgccacctc
     3481 aatcgccggt ttgttctgca atacggtcca gataaccagc gcacccagca ggtcaaatac
     3541 tgccagaact gcgaacagcg ggctgaagcc gatggtgtca gccagtgcac cgacaaccag
     3601 cgcaaacagc gtacttgcca gccatgcgga catcccggtt aaaccgttcg ccgtcgccac
     3661 ttcgttacga ccgaacacgt cagaagagag cgtaatcagc gcgccagaca gtgcctggtg
     3721 ggcaaaacca ccgatacaca gcagcataat tgcgacatac gggttggtga acagaccgat
     3781 catacccggg ccaatcatca gcacagcacc cagcgttacg accatcttac gggaaacgat
     3841 caggttcaca ccaaaccaac gctggaacag cggcggcagg taaccaccga ggatacaacc
     3901 gaggtcagca aacagcatcg gcatccaggc gaacatcgcg atctctttca ggttaaagcc
     3961 gtaaacttta aacatgaaca gcgggatcca cgcgttaaaa gtaccccagg ccggttctgc
     4021 aaggaaacgc ggcagtgcga taccccagaa ctgacggtta cgcaggatct gaccaacgga
     4081 cattttcttc gccgtgctca cctggtgctg ggcttcctga ccattaataa tatagtcgcg
     4141 ttcttcatcg gtcagatgct tctggtcgcg cggatgttta tagaaaatca gccatgccat
     4201 cgcccagata aagctcaatg caccggagat gataaatgcc atctgccagc tgtgcatcac
     4261 gattgcccat accaccagcg gcggcgcaat catcgcacca atcgaagaac ctacgttaaa
     4321 gtagcctact gcgatggaac gctctttcgc cgggaaccat tcggagctgg ctttcagacc
     4381 cgccgggatc atcgctgctt ccgcggcacc gaccgcacca cgagcaacag ctaggccacc
     4441 ccagctacct gccagcgcgg ttgcaccaca gaacacggcc cacagcacag caaacattgc
     4501 ataaccgatt ttcgtaccca gcacatccag tacataacct gctaccggtt gcatgaccgt
     4561 ataagcagca gaataggctg cgatgatata ggaatactgt tgggtggaga tgtttaactc
     4621 ttccatcaga gttggcgcag ctgccgccac agtgttacgc gtcaggtaac caagcacggt
     4681 gccgagcgtc accagtgcga tcatatacca acgtaaccct ttaattttac gcatgtaaaa
     4741 cctcatcgtt ttgttatatc cgcaggaagc gcggccatca tcccgccaga ggctgaagat
     4801 gttagttacg accgggtaac cgaaaaaaaa accggcacgc cccgaacgtt gccatgaaag
     4861 tttcgtgcta attcggcttc cgtaccggtt aacccgattc atctaacaaa tgaatgaatc
     4921 ggcaatttgc attccgtcga cttatgattt gcgacggcag aaagataact tgtcatacaa
     4981 ccttaaaagg tgagagccat cacaaatgtg cgaatatttg tagggacatt acctgacgac
     5041 agcgaggcca gtactggcgc ggcctgcagc gagatttacc actttgagag taattttttt
     5101 aactacgttt attgatctaa ctcaagaaaa tatcttcgga ctctggaaat tggtgtgata
     5161 actttgtcag catcgcacca taagcaagct agctcactcg ttgagaggaa gacgaaaatg
     5221 actccgttta tgactgaaga tttcctgtta gataccgaat ttgcccgccg tctgtatcac
     5281 gactacgcaa aagaccagcc gattttcgat taccattgcc atttgccgcc gcagcagatt
     5341 gcggaagact atcgttttaa aaacctgtat gacatctggt tgaagggcga tcactacaaa
     5401 tggcgcgcta tgcgtaccaa cggtgtggct gagcgtctgt gtactggtga tgcgtctgac
     5461 cgtgaaaaat ttgacgcctg ggcggcgact gttccgcaca ctatcggcaa cccgttatac
     5521 cactggacgc acctcgaact gcgtcgtccg ttcggtatca ctggcaaatt gctttctccg
     5581 tcaactgccg atgaaatctg gaacgaatgt aacgaactgc tggcgcagga taacttctcc
     5641 gcgcgcggca tcatgcagca gatgaacgtg aaaatggtcg gcaccaccga tgacccgatc
     5701 gattctctgg agcatcacgc agagatcgcc aaagacggct ctttcaccat caaagtgctg
     5761 ccgagctggc gtccggacaa agccttcaat atcgaacagg cgacctttaa cgactacatg
     5821 gcgaagctgg gcgaagtttc cgataccgac attcgccgct ttgctgacct gcaaactgcc
     5881 ttgaccaaac gtctggatca cttcgccgct cacggctgta aagtgtctga ccatgcgctg
     5941 gacgtggtga tgtttgctga agcgaacgaa gcggaactgg acagcattct ggcgcgccgt
     6001 ctggctggcg aaaccctgag cgagcacgaa gtggcacagt tcaaaactgc ggtactggtg
     6061 ttccttggtg ccgaatatgc acgtcgcggc tgggtacagc agtaccacat tggcgcgctg
     6121 cgtaataaca acctgcgtca gttcaaactg ctggggccgg atgtaggctt tgactccatc
     6181 aacgaccgtc cgatggcgga agagttgtct aagctgctga gcaagcagaa cgaagaaaac
     6241 ctgctgccga aaaccattct ctactgcctg aacccgcgcg ataacgaagt gctgggcacc
     6301 atgatcggta acttccaggg cgaaggtatg ccgggcaaaa tgcagttcgg ttccggctgg
     6361 tggtttaacg atcagaaaga cggtatggaa cgtcagatga cccaactggc gcagctcggt
     6421 ctgctgagcc gctttgtcgg tatgctgact gacagccgta gcttcctgtc atacacccgt
     6481 cacgaatact tccgccgcat tctgtgccag atgatcggtc gctgggtgga agcgggcgaa
     6541 gcaccggcgg acatcaacct gctgggcgag atggtgaaaa atatttgctt taacaatgcg
     6601 cgtgactact tcgccattga actgaactaa ggtctggggt tgatatgcaa tacatcaaga
     6661 tccatgcgct ggataacgtc gcggtcgctt tagcagattt ggctgaaggc acagaagtca
     6721 gtgtcgataa ccagactgtt acgctgcgcc aggatgttgc tcgtggacat aaatttgcgt
     6781 taacggatat cgcaaaaggg gccaacgtca tcaaatatgg cctgccgatt ggttatgcat
     6841 tggcggatat tgcggcgggg gaacacgtcc acgcccacaa tacgcgcacg aatctgagcg
     6901 atctggatca gtatcgctat caacctgatt ttcaggatct gcctgcgcaa gcggcagatc
     6961 ctgaagtgca gatctatcgt cgcgctaacg gcgatgtcgg ggtgcgtaac gagctgtgga
     7021 tcctgccaac cgtgggctgt gtcaacggca tcgcgcggca aatccagaac cgtttcctga
     7081 aagagaccaa caacgccgaa ggtaccgacg gcgtgttcct cttcagccac acctatggct
     7141 gctcacaact gggcgacgat cacatcaaca cccgcaccat gctgcaaaac atggtgcgcc
     7201 acccgaacgc gggcgcagtg ctggtgattg gtctgggctg tgaaaacaac caggttgccg
     7261 cattccgtga aacgttgggc gatatcgatc ctgaacgcgt tcatttcatg atctgccaac
     7321 agcaggatga tgagatcgaa gccgggatcg agcatttgca tcagctgtat aacgtgatgc
     7381 gcaacgataa acgcgagcca ggcaaactca gcgaactgaa gtttggtctg gagtgcggtg
     7441 gttctgacgg tctttctggt attactgcta acccgatgct ggggcgtttc tctgactacg
     7501 tgattgctaa cggcggtact accgtactga ccgaagtgcc ggagatgttt ggcgcagagc
     7561 agttgctgat ggaccattgc cgcgacgaag caacgtttga aaaactggtc accatggtca
     7621 acgacttcaa acagtacttt attgcccatg atcagccgat ctacgaaaac ccatcaccgg
     7681 ggaacaaagc gggcggtatc accacgctgg aagacaaatc acttggctgt acccagaaag
     7741 cgggttccag tgtcgtggtt gacgtgctgc gttacggcga gcgtctgaaa acgccagggc
     7801 tgaacttgtt aagtgcgccg ggtaacgatg ccgtagcgac cagcgccctg gcgggtgcgg
     7861 gctgccatat ggtgctgttc agtactggtc gcggcacgcc atacggtgga tttgtgccga
     7921 cggtgaaaat cgccaccaac agtgaactgg cggcgaagaa aaaacactgg atcgactttg
     7981 acgcgggtca gctgatccac ggtaaagcga tgccgcagtt gctggaagaa tttatcgaca
     8041 ccatcgttga gtttgccaac ggtaagcaaa cctgcaacga gcgtaacgac ttccgcgaac
     8101 tggcgatctt caaaagcggc gtaacgctat aaagtcgaaa tgagttgtta ataaaaaacg
     8161 gcgtttcata atgtgaagcg ccgtttgctt ttccatacct gctaaggatt tatcatgacc
     8221 gcgtattggc tggcccaggg cgtgggtgtc atcgcctttc tgattggtat cacaacattt
     8281 ttcaatcgtg acgaacgtcg cttcaaaaag cagctttcgg tctatagcgc cgttattggc
     8341 gtacattttt ttcttctggg cacctatccc gctggtgcca gtgccatcct taatgccatt
     8401 cgtacattga ttaccttacg cacgcgcagc ttatgggtaa tggcgatttt tattgtgctg
     8461 actggcggaa ttggcctcgc gaagttccat catcctgtcg aactattgcc ggttatcggt
     8521 acgattgtca gtacctgggc gctgttccgc tgtaaagggc tcaccatgcg ctgcgtgatg
     8581 tggttttcaa cgtgttgctg ggtgattcac aacttctggg cggggtcgat aggcggcacg
     8641 atgattgagg gtagttttct gcttatgaat ggcctgaata tcattcgttt ctggcggatg
     8701 cagaaaaggg gaattgatcc gtttaaagta gagaaaaccc ctcccgccat agacgaaagg
     8761 ggttaaacaa ttaattacgc agggcgctat ttgccagacg atcgtcttcc gcctggcaag
     8821 ctgccgcagt gaacagcacg tcagttgaag agttcagcgc ggtttcgcaa gagtcctgca
     8881 atacgccgat aataaagccg acggcaacca cctgcatggc gatatcgttc gaaataccga
     8941 acatattaca ggccagtggg atcagcagca aagaaccccc cgccacgccg gatgcgccac
     9001 aggcacacag agaagccact acgctcaaca gcagcgctgt gggcagatcg accggaatac
     9061 ccagcgtatt aaccgcagcc agcgtcaaca cggtaatggt gattgctgcg cccgccatat
     9121 tgatggtggc tcccagcgga atagaaactg aataggtatc gcgatccaga ttcagctttt
     9181 cacacagcgc catattcacc ggaatgttcg ctgcagagct gcgggtgaag aaggcataca
     9241 caccgctttc gcgcaggcac agcagcacca gcgggaacgg gttacgacga attttccacc
     9301 acaccagcaa tgggttaacc accagcgcca ccagtaacat acagccaacc agcacgacca
     9361 gcagttgcgc gtagccccac agtgtggaga aaccggtggt tgccagggta gaagaaacca
     9421 gcccaaaaat accgaacggt gcgaagcgaa tgaccagttt caccataaag gtaacggcat
     9481 tcgacatatc attgaccagg tttttggtgg tctcgttacc gtgacgcagt gcgaagccga
     9541 ggccgatcgc ccacaccaga atcccgatgt agttaccttt cagcagggcg tcgatggggt
     9601 tggaaaccat gctcattacc agtccgcgca tcacttcgac aatgcctgac ggcggcgaaa
     9661 tatcacccgc gctactggac aagtgcaggg tagaagggaa ggcaaagctg aagactactg
     9721 cggccagagc tgcagagaag gtgcccagca gatagaggaa caaaatagga cggatattgg
     9781 ttttctgccc gtgctggtgg ttagcaatag atgccattac cagcatcaac accaggatgg
     9841 gggcaacagc tttcagtgcg ccgacgaaca aagtacctaa cagaccaaca gcttccgccg
     9901 cgggttttga gatccatgcc agaagaatcc ccagaacaag gccgaccagg atttgtttta
     9961 ccaggctgcc atgagccaga cgccggaata gccccggtga acgttgcgta gtcatttttc
    10021 gatcctttca ttgtgttgtc gcacatccct ggtgcgttct gtcgacgcat ctttcaggat
    10081 gtaacaaagt gtttgcaagg tcgagtataa ggaaagttac agcttcggga agcgaaaaat
    10141 gctggatttt aaagagaggt catgttttgc aacttaatgg ttgcatttat ttaacataaa
    10201 cagagggaag gactggctac taaggctgat tagataacat atgggcaaaa ctatttggaa
    10261 tatgagaacg gttgcgctcc tttaacgtgt tatgccatat aaagtcactg gcgcagggag
    10321 cgcgcagggg gcggccaatc gccgccgccc cctgcacccc caggctctgg cgaacaaaat
    10381 cgccgctgcg cggttccctc agcctatccc ttgcggctac cgggtcgggc gcaaggtaac
    10441 gtccctgtaa gatgcgccct cgccccggcc tccagggaac gcttcggcga ttttgacgcc
    10501 accaacaccc gtgcggtctt tttgtaaaga gctataagcc gattacataa ttaatagctc
    10561 acctttgtct ggaatgagtt atctctagga atggcttaat atacagcgaa tcattctccc
    10621 cgctgcttat catgccgata attcacccag gcgttgataa taaacgtcat caccagaatg
    10681 ccaaacacca cgcccagcga gacggcgatt ggaatatggt agaagtcgac aatcagcatc
    10741 ttgataccga taaacaccag aatcaccgcc aggccatatt tgagcatcga gaaacgctct
    10801 gctacgcccg ccagcaggaa atacatcgca cgcaggccga ggattgcaaa caggtttgag
    10861 gtcagcacaa tgaacgggtc agtggtcacg gcgaagatag ccggaatgct atccacggca
    10921 aaaatcacgt cgctcaattc caccagaatc agtaccagca tcagcggtgt ggcgtacagc
    10981 aatccattct tacgcacaaa gaaatgctcg ttgtcgatgg tgtcggtcat gcgcaaatga
    11041 ccgcgtagcc agcgcaccag cggcttgtcg ccaatgcctg attcatcttc atgggcgagc
    11101 gccatcttca cgccggtaaa cagcaggaag gcaccgaaga tatacaggat ccagtcgaac
    11161 tgcgaaatca gccagctgcc tgtgaagatc atgatggtac gcagaacaat cgccccgagc
    11221 acgccataca ccagcacgcg acgttgtaat gccgccggaa cggagaaata gctgaacaac
    11281 atcagccaga caaagacgtt atcgaccgcc agcgatttct caatcagata acccgtgaga
    11341 aaggccaggg cctgtggatc ggcaacggcg cgaccttcgg tttgcaccag ataccaccag
    11401 aaagcggcgt taaacagtaa cgacagcgtc acccagacca gcgaccaggc cgcagcctgt
    11461 ttcatggtca tggcatgtgc cccacgacgc ccctgcaaca acaggtcgat agccagcata
    11521 atggcgacaa caacagcgaa tccgccccat agcaacggcg tgccgacagt attcatagaa
    11581 gttccttaca cataaaaaaa cggccaacgt cggaagacgt cagccgctgc tttttatgca
    11641 tagacctcgc cttccggcaa ggtctcactt acaacaaaaa aggaatacgt ctgcgtattc
    11701 ctttcgggtt gcccggtgac cggatgtggt ttttcacaca tcgtaatgac gatcgaccgg
    11761 caatgaagtt actccccttt gcaggtaaca aagtatgaca gaccattcag tcagtcaatg
    11821 gtctgttaca taccttttac actgttttac tttggcaagt atagttttac gctatctgcc
    11881 ggaaaaatca ccccagtttg gcggcggatc tcggtcagca gtttggctgt gatgcgactg
    11941 accgccagcc ctggatgatc caccagatgc tcatccacca gggtagcgaa cagctctgct
    12001 tcgtagagca tggtattaat atgctgcggc tgggtgagat cctgcatctg gctgccacgc
    12061 ggcacaaagc agactttctg gcattcagac agtttttcaa tcaccagcga tcccgcttcg
    12121 ccctgaatct cgcttcccag gacagaatca ctgacctttg agtgctgcaa ggtgacgctg
    12181 aaatcaccgt aatccatcac caccacgccg tgggcgtcaa cgccgcttgc cagcaaactc
    12241 gccgttgcct gcacgctttt cggctcacca aataacgcca ccgccgacgc caggcagtaa
    12301 aagccgatat ccataattga accgttagag aatgccggat taaaggtgtt gggattctca
    12361 ccgtccaggt aacgttgata ccgcgaggaa tactggcaat agttgaaaaa gactttacgc
    12421 agttttcctg ctttcggcag cgcctggcgc aacaaatgaa agttcggcag gcaggcggtt
    12481 ttaaatgcct caaacagcac cacctgattt tcgcgcgcac aggcaatggc ggcatccact
    12541 tccgccagat tcgacgccag cggtttttcg caaatcacat gaattttatg gctaaggaaa
    12601 agttgtgtct gggaaaagtg cagggaattc gggctggcaa tatacaccgc gtcaatggca
    12661 tcgctttccg ccatcgcttc cagcgacgta aacagatgct cgacagaaaa atcattggcg
    12721 aagtgctgag cctgctcaag gctgcgggaa tatacggcgg ttaacttgta tttaccgctc
    12781 tcatgggcgg cctcgacgaa ctggcgagtg atccagttcg taccaatcac agcgaaacgt
    12841 atcataacgc gtggatactc cataaaggga ttctgtcgtc aatgtagcac gcctttttca
    12901 tcttcgacgt gcgcagatac gcattattct ttcgccggac agccatgctc actaaacggc
    12961 ccaatcatga ccgatggccc gagatatttc ggctggcgat ggaggatttt tgcgtcaatc
    13021 accgcgctga cacgagcgag tttctcccgc agttgagtat aaaaaccgtg cttcatattg
    13081 aggtctggcg cgttaaaccc gatagcgtcg atgccgtact gttttgccag ccagatggcg
    13141 cgttggttat gaaattcttg cgagataatg gtgatatggt tttccccaaa cacctttttg
    13201 gcacgcacta ccgaatccag cgttgagaat ccggcatagt cgcaaaagat cactttcgcc
    13261 ggtacgcctt tagcgatcaa agcctgctgc atacctgatg cttcatcgta atttttacgt
    13321 ccgttatcgc cactcaccag tagccatttc acttttccgg cgtggtacaa cgctgctgcg
    13381 gtatcaatac gtcgggtaaa gtagcgatta cccggcctcg cccccagcaa taaacccacg
    13441 ttacgcgccg ggacggcgtt gacatcgccc caggtcagct gcttgctggc gttgaccata
    13501 acgcgatcgg cgataaaaat ggtcgctcct gccacaagta acagtaagca ggcaatcttt
    13561 agagtccggc gagaaaaaca aatacggaga agaaggcggg caaatgcgcg cagcatggtc
    13621 gttatcctta acgaaagggg ggaggtcgat tttaggcgct aaagaaaaga taacgcctga
    13681 catcattttc agattttgtc ctgcccaagc gacaactgcg ttagccacag ccgggtgtcg
    13741 aactcaagct ggtggtactg cggctccata tggcagcaca attgatagaa cgctttgtta
    13801 tgctctttct ccttcaggtg cgccagctcg tgcaccacga tcatgcgcaa aaacggttcc
    13861 ggcgcgttgc gaaacacggt agcgacgcgg atctctgctt ttgctttcag cttgccgccc
    13921 tgcacacgag aaacagcggt atgtaacccg agcgcgtttt ttagcacatg gatcttattg
    13981 tcatacatca ctttattgat cggcggggca ttacgcagaa actgattttt cagatcctga
    14041 gtatattgcc agagagcttt atcggtggcg tagtcgtgtg ttcccggata gcgttttgcc
    14101 agcacatcgc ccagacgctg ttcgttaatc agtgttcgca cctgcgaaag cagctgctcg
    14161 ggataaccct gaagataagt aagattgctc ataaccgccc acgaaaaaga gaaaaagggt
    14221 atactcacgc acccttttca ggggcaacgt cgaaatttta tcattcagga gggccgatga
    14281 gccacttaga caacggtttc cgttcactga cactacaacg ttttccggcg acggatgacg
    14341 ttaacccgct acaggcgtgg gaagcggcag atgaatattt gctgcaacag ttggacgaca
    14401 cagaaatccc cggcccggtg ttgatcctga atgatgcctt tggtgcgtta agctgcgcgc
    14461 tggcggaaca taagccgtac agcattggcg actcatacat cagtgaactg gcgacgcgcg
    14521 agaatttacg cctaaacggg attgatgagt cgagcgtgaa gtttctcgac agcaccgccg
    14581 actacccgca acagccgggt gtggtgctga tcaaagtgcc gaaaacactg gcattgctgg
    14641 aacagcaact gcgtgcgctg cgcaaagtgg tcacgtcgga tacacgtatt attgccggtg
    14701 ccaaagcccg tgacattcac acttccacgc tggaactgtt cgaaaaagtg ctcggcccga
    14761 ccaccaccac gctggcatgg aaaaaagcgc gcctgattaa ttgcacgttc aatgaaccgc
    14821 cgctggccga tgcgccgcag accgttagct ggaagctgga aggtactgac tggactatcc
    14881 ataaccatgc gaatgtcttc tcccgcaccg ggcttgatat cggcgcgcgc ttctttatgc
    14941 aacatctgcc agagaatctc gaaggggaga ttgtcgatct cggttgcggt aatggcgtta
    15001 ttggtctgac gctgcttgat aaaaacccgc aggcgaaagt ggtgtttgtc gatgaatcgc
    15061 cgatggcggt tgcttccagc cgtttgaacg ttgaaaccaa catgccagag gcgttggatc
    15121 gcagcgagtt tatgatcaac aacgcgctct ccggcgtgga gcctttccgc tttaatgctg
    15181 tgctctgcaa ccctccgttt caccaacaac atgcgctgac cgataacgtc gcctgggaga
    15241 tgttccacta cgcccgccgc tgcctgaaaa tcaacggcga gctgtatatc gttgccaacc
    15301 gccatctgga ttacttccat aaactgaaga aaattttcgg caactgcacc actattgcga
    15361 cgaataataa atttgtggtg ctgaaagcgg tgaagctggg acgtcgtcgg taagattggg
    15421 agttacctgc cggatgcggc gtaaacgcct tatccggcct acgttcagca cgttacccct
    15481 aggccggata agacgcggca agcgtcgcat ccggcaatgt ctgctcagtt ctggaatcgc
    15541 gcttccataa aacaaaaaat ccctgtcacc atcctctaca ttctcttgtt tagcgttttt
    15601 ctacgtttat tcttccgtca cacagataaa ttcatccgtt gccaatctgt caacggatgt
    15661 tatcatattg ccaatttgtc aacggacgtg atgaagaatg cacctgataa ctcaaaaagc
    15721 attgaaagat gctgcggaaa aatacccgca acataaaacg gagttggtgg ctctggggaa
    15781 cacgattgct aagggatatt tcaaaaaacc tgagtcatta aaagcagtat tcccatctct
    15841 ggataacttc aaatatctgg ataagcatta tgttttcaat gttgggggca atgaattacg
    15901 tgttgtagca atggtctttt ttgaatcgca aaagtgctac atacgtgaag ttatgacgca
    15961 taaagaatac gatttcttta ccgctgttca tcgtactaag gggaaaaaat gattgcgatt
    16021 gccgacatct tgcaagcagg agaaaagcta actgctgtgg caccttttct ggcgggtatt
    16081 cagaacgagg aacaatacac ccaggcgctg gaactggtag atcatctgct gctcaacgat
    16141 cctgaaaacc ccttgctgga tctggtgtgt gccaaaataa ccgcgtggga agaatcagcg
    16201 cccgaatttg cggaatttaa tgccatggct caagccatgc ctggcggtat agccgtgatt
    16261 cgtaccctta tggatcaata tggtttaacc ctttccgatc tgccggaaat tggcagtaaa
    16321 tctatggtgt cacgcgtttt gagcgggaag aggaaattaa cgctggaaca cgctaaaaaa
    16381 ttggcaacgc gattcggcat ttctcccgcc ttgtttattg attaacgcga cgtgccggat
    16441 gcgttgcatc cggcgcttca aataacgatt taaatctcca gcgccagccg tgttccctgg
    16501 gcaattgccc gtcgtgcgtc cagctccata gccacatcgc agccgccaat taaatgcaca
    16561 gttttcccgc tatcaatcag cggttgcgcc agcgcgcggt ttggctcttg ccctgcgcag
    16621 atcaccacat tgtccactgc taatacctgc gtttcgccgt tgatcaccac atgcagcccg
    16681 tcatcgtcaa tcttctgata acttacgcct gggatcattt tcacaccccg cgagagcagg
    16741 atggtgcgat ggatccagcc ggtggttttg cctaacccct gtcccggttt gctggctttg
    16801 cgctggagca tcacaatttg ccgtgggcta cgcgggatct gcattccctg cgggcttaag
    16861 ccaccagcct gttgtaggct gctgtcgatc ccccactcat tacagaacct ggcgatattc
    16921 tggctggtgg attcgcccgg ctgacttaaa tacatcgccg tatcaaaacc aatcccgcca
    16981 caaccgatga tggcaacctt gttgcctacc ggcgctttgt cgcgcagtac atcgagataa
    17041 ctcaatacct tcggatgatc gatcccgtcg ataggcggaa cacgcggcac gatcccactg
    17101 gcgaggatcg tttcatcgaa cgcctgtaac tgatccgccg tcacggtgtg attgagtttc
    17161 agcgtcacgc ccgtcacttc gatcatccgg cggtagtagc gcagcgtttc gtaaaactcc
    17221 tctttgccgg ggatctgttt ggcgatatta aactgcccgc cgatctcgct gtgcgcatca
    17281 aacaatgtta cctgatgccc acgcgccgcc gcgttaatgg caaacgccag cccagcaggt
    17341 ccagcgccga ccaccgccag attttttttc tgcacggcgg gaaggattgg cattttggtt
    17401 tcgtggcagg cgcgaggatt caccaggcac gaggtgactt tgccaacgaa gatctgatcg
    17461 aggcagccct ggttgcagcc aatacaagtg ttgatctcat cggctcgccc tgattgcgct
    17521 tttgacagca gttccgcatc cgcgagaaac ggtcgcgcca tcgatactat atcggcatcg
    17581 ccgcgcgaga gaatatcgtc ggcaacctgc ggatcgttaa tccggttggt ggttaccagc
    17641 ggcagcgaga cgtggccttt cagtttgcgc gtgacccagc taaatgcgcc gcgcggcacg
    17701 ggcgtggcga tggtcggaat ccgcgcttcg tgccagccta ttccggtgtt gataatggtc
    17761 gcgcccgccg cttcaatggc ctgcgccagt tctaccgttt cggcaaaagt gccgccgcct
    17821 tcgaccaggt cgagcatcga caaccggtaa ataataatga agtcgttgcc gacgcgttcg
    17881 cgcaccgtac gcactacttc tacggcaaat cgcatccggt tgcggtaatc gccgccccac
    17941 tggtcactac gctgattggt gcgcagcgtc agaaattcgt tgatcaaata cccttcagag
    18001 cccatcactt cgacgccatc gtatccggcc tcccgcgcca gttgcgcgca gcgggcgaaa
    18061 tcgtcgatca gttgcaggat ctcttcatgg gtgagttcgt gaggaacgaa acggttgatg
    18121 ggggcctgca atgcggacgg tgcgaccaga tgcggttggt agctgtagcg cccggtatgc
    18181 aaaatttgca gggcgatttt gccgccttcc tgatgtacgg cttcggtaat ggtgcgatgg
    18241 tgtgggatct ggctggcgtc gttgagcatt gcgccacctt ccatgccaac gcctgttaaa
    18301 tctggtgcaa tgccgccgct gacaatcagc gccacgccgt gacgggcgcg ttcggcataa
    18361 aacgcagcca gccgttcagc accgtccggg tattcctcca gcccggtgtg cattgaaccc
    18421 atcaacacgc ggttttttaa cgtggtaaaa cctaaatcca gcggggcgaa cagcgacggg
    18481 tagctcataa aattgtccag tatgtaaaat aattgttatg tagtcggatg agttctaatt
    18541 tagccgtcga tggagaaaag ggaaaagggg agtgcggggt ttgtgatggg attcaaaaag
    18601 aatacgcccg gtggtaacgc cggatgcgac gcttgccgtg ttttatccgg cttgggggat
    18661 tgcacatgta gggcggataa ggcgttcacg ccgcatccgc cagccgttgc aaattactgc
    18721 ttacggaaaa aatcgttata caacatatac aaatgagcgg cactccagga gaaatttggt
    18781 gcgccttgct gcgcgcctgt cagcgggttg taattctcct gaattgggcc atcggcggtt
    18841 aatcctttgg cgtgcctgaa gaacgtatcc gccagcttca gggcatcatc acgataaccg
    18901 taacgctcca tccctttcag accaaaccag aactgatcca cccatacgcg cccgcgccag
    18961 tagatatcag cgccaaaagc cggattggtt aacgccgccg ttcccagcgg aacaaaggtg
    19021 ttgaactctt taggatcgag catcaccttc accaccgcgt cggcattggc ctgcgttgcc
    19081 gcaccgttaa acagcggcga ccagccttcc ggccctttac cgcgctcaac aatcggtttg
    19141 cccgcgcagc cgttcgccag tggtttatct tcaatacgta cgtcatagta gaactgggta
    19201 gtcgggtcga acatacaggt gttgatgtag tccgcgagct gctgtgccaa ctggcgatag
    19261 cgtttggcct cttctggctt accgaggatg gttgccatct ccgccagata atggttatcg
    19321 ctgtacatat agctggcctg atccaccgac tcctgcaata gcgagtagcc cagcaacgtt
    19381 ccgtcctgac tgcggttttc ggcgaatttc accgtccagt cgctacgttt gccgccatta
    19441 gcgacatatt tatccaactg ttctttgtcg ataaaaccaa agacggcggc atcatcacgg
    19501 cccgattccc acgacgcagc gacctgtgcc ggaatttcca gactgtcgta ctggcctttc
    19561 tccaccacgc gggcgtagtt gttcagccca gactgcgtct cttctttatc gccttttttc
    19621 accgtaaaca gcatctcgcc gctctcggtg ttgtgggctt tgtcgcgggt cgcgccatat
    19681 tccggcaccc cgttgccgtt atgatcgcgg ttacgtaacc accagtcgtg ataggccacc
    19741 agtttcgggt acatctctgc caaccaggct ttatcctggg tgacgttgta tacttccatc
    19801 accgaccagg cggcaaggct gggtttggta ttacgttcgt tccagttgcc gccatcgcca
    19861 ccacgctcgg ggctaaggtt ccacgctatc aggtcgggga caaagcccac atcctgcgga
    19921 cgcacgctgt cgccaggctg gatctgccag gagaagaccg cgcggatatt ctctttggcg
    19981 atatccggat tgaaatgcgc catcgcaaac gcctgcttcc aggtatccca cggccaggtc
    20041 tgattgccgg agaaccagcg tccggtcacc gacggcgtga cggtgttata attcaccgca
    20101 ccgcccggtg agcgccagtt accgttgagc gtttcgatcg ctttcaccgc gacgcgcgtc
    20161 tgttccggcg tcgcatccgg attggttaac cctttcttca ggtattcttc ccagcgttgc
    20221 tgcgaggcgg tgagataaaa cgccggacgc gccagaatat cgcggatctg catttgctct
    20281 ttgctaactt cctgagcggt cagcagatgg gaataggtgg tgtagagcgt ggtcgaaccg
    20341 ttgatatgcg ccttactggt aaagcgatta ccgttgattt cagtctgcac cggcagggat
    20401 ttatgcacct gatattccga ttcgccggag gtcagcagat cccaggtggc gcgcactttg
    20461 ccaaaggtga ctttcaggcc atcacgggtg gcgctgattt tgcgctgata gtcagggtat
    20521 tcgccagcaa tggttttatc ggaaagcggt ttcccttctt tcgcttccag tttttccagc
    20581 agttcgccat cccacaccag atccagcggt ttattgctga tgattttagt ttccagtagt
    20641 gacgtgcgcg gcgtggcgaa gcgcagagtc atttcgacct gcacatcttt tgctgtcagt
    20701 ttttgcacca gcgcgccggg aatactgtat gcctccagcg tgaagttgac tttcttgccg
    20761 tcttgccaga cagtcaggcg gtcgaaattg ctggccataa agttgatgta ttcttccgtc
    20821 agcagcgcaa cgcccggaaa gccgcccatg gtgttcgggc cgtctggcaa cagatgacca
    20881 tgccaggctc cgagatcgaa aaacggatta aaacgctggt gatcgtcgta atcgtaatcc
    20941 ttcatgtact gcggcgcacc agtacggtta atcacgtttt tataattgtc ggcgttagcg
    21001 gcatgagcgg aaaaacttat cagcagagcg caggttactg gcgttaaaat agttttgatt
    21061 ttcatgagat tacagtcctt attaccagta gaaatattgc atcaggaaga tttggtcgct
    21121 gggatcgtta gaggcatttt tcatgtagaa gcggctatca aaagccgtgg cgaaacgtcc
    21181 gccgccatat tcgtaccaga taccaaactg catgaaagag gtggtttcat cgtcagcgtg
    21241 gtcagggcga tgtttggcgt aggtgtaagc ggtggagaga taaacgcctt ttaacgcttc
    21301 atacatggcg ctcaccatca tcccgctttc acttcccggt acgtccagcc cgtcggcacg
    21361 tccggtatgg tgccaggcgc gagcggcaaa gttaggggta aacaggcccg tgagatagag
    21421 ttgtcctgtg ccgtcggtaa tttccagacc attcatccag gtgatgcctt cggcaaggtc
    21481 atactggatg taaccgttca tccattgccg gataggtgta tttgtcgtcg tagcgatcgt
    21541 acttgccaaa atgtagccag gctttgcttt cgtcatgatg tccggcaggt gtggcggtga
    21601 cgctataacg aaagttgccg cttaagttct gtaccttcag cgcggtcata atgtcgcggg
    21661 tattgggaat gacgtagccc aaatcgggtg tgaagtcgcc ccaccattgc aggtcatcca
    21721 gcgaggaatc ctgacgcaga ctgagcatga tttcggtgcc atcattggta cgataaccgc
    21781 catagaaacg gttaatccca ccttcgaaac caccccagct atggtcaggc acctaggcat
    21841 taccgtcgtg gtcggcctgc acggtccagc cttcgccgta aatcaggccg aaccaggagc
    21901 cgtacttcat ctccaggcca ccttcaatat aggtgccgtc gctgtattta tgtttattct
    21961 caccctctaa atccatatat ccaccaagcc ctaattcgcc gtaaaaacga aatgaaggag
    22021 aagatgtcga ggtaattgcg ggtgtttttg tgtcgtcatg ttctgccgga cgtgcttctt
    22081 ctcctgccgc agaaaaggcg tagcaaaacg gcagagcaag taatgctctg catggtgcag
    22141 tgattaattt cattgtgtta atccatgagc taaagaaaaa gtacagcctt ttatcgttgt
    22201 aattataaaa atataaaggc agaaactatt ttccctctcc tcatccagga agagggaaaa
    22261 tgcctgctca ttttttatta gtctgcttaa tcaacattgc tggctggcgt cgcttcaata
    22321 tggcgctctt cagccgttaa tccctttata tatttgctgt atttccacca cgcaaagccg
    22381 aggaagataa caataccgcc gacgttataa aaaatgatgg tcagaatatt cgcgccagtc
    22441 gggaatgtcg aagcgacaaa ccctacggca aatatcgcaa tcagcattga aacaacgata
    22501 ataccggtgc ggcgggagcc catacggaaa tcgcgtggca ggtgatctaa tttggcgcgt
    22561 aaattcaggt aagccagcat gatgaataac ggcggaagca tggacgctgc ggcggtcata
    22621 ttaataatgg tattcatcag atcctgcacg gtattggaac cgagcatcgg gataatcatc
    22681 agcgggatga cgatcagaaa ctggatccat gctgcgcgcg ccggaacgcc gttttcgtta
    22741 agttcgacgg ttttcttacc aaagatgcct tccgggattt cggagaagaa aattttcacc
    22801 ggcgttgcgg tccacatcag cagggaaccg aacattgcgg taaaggacac cagaccgaca
    22861 aagcgattca tcaacgcttc cggcagacca aaatacgccg ccatgccgtg gaatacctgc
    22921 accgatccgc cggtaaattt caactctttg ctgctgacga agacgttaat cagcaccgag
    22981 gagacggaat acagtacgcc gataaaaatc ccggcgagga tgatcacttt aacgaacgat
    23041 ttcgaaccgc ctttgacgtc gttaacgtac accgcgacgg actccgcgcc acctgcggcc
    23101 ataaagatcc aggtggtaac gccgaggaac gcccagttga agttcgggat catcgcgtca
    23161 acggtgatcg gatcggcagg ctgtacgccg ccaaccagtg ccgtacctgc cagtaaaatg
    23221 taggagagcg ttaacagcag catcagcgtt gaagtgacgg aggtaattgg ccccagcatt
    23281 ttcgccccgt tggtggaaac ccaggtggag aaggcgaaca gcaccatact gataacggtg
    23341 gtggcaaccg gcgtcataat atattcgtag ccgaggaagg catacgaagc ataagcaata
    23401 acgcgcggca acagcgaggt gaaaaagaac agatttacga accagtaagt gtaggcagta
    23461 ataaatgccc aacgtccgcc cagcgaactt tttacccacg cgtagacacc ggcttctgag
    23521 tttttattta acgaaacaaa ttctgcgatg atcagacaga agggaataaa ataaaaaatc
    23581 gtcgcgagga aaaacatcgg tgccgaggcc agtccaagct caatattatt attgataacg
    23641 ttgttaaagc tgtaaacggc ggcaaaagtc agcgagagca agccgaattt gccgattgta
    23701 ttacgtttgg tatcagacat aacacatctc cgaaaatgtg tagggtagcg gtggcggcaa
    23761 actccttttg ccgccgtagt tgttatttgt tatggaaata accatcttcg atggtgactt
    23821 tgagaaccac ttttttgacc gcgttattgc agataaaccg atacgcttca tggatatcgc
    23881 aaataacgat ttgcccttcg tggacctcta cggtttctcc gcagcctttt aaatattcac
    23941 ggtcagtttc atcacgataa tattccacta cctgtaatgt ctctttcggc gcatattcaa
    24001 ttttttgctg cccttgcagg tagtaatgca cttcaaaata gcgtcgatgc ccggtaaata
    24061 gcgcatcggt cgcggagtca ttttccacac ggtaggtcaa tgaatcaccg atggagtggg
    24121 cgacgccagg ctgaatgttg tcgatatttt caatcgcttc aacgcagcgt tgccacttct
    24181 tgccagaggc gtaaatctgg cggaactgtt ctaagttatc gatgatcctc attgcttatt
    24241 ctcgctgtgc aaattcgtgg aaaagaaccc tgcgccgaac tcatacgacg ccaggctttg
    24301 cgcggtagct tctccgccag aaaccggcag caacgtaaag ccgtagctga agtcacggaa
    24361 ccagacgcgc caggagtcca gcacctcgct gccccaggag ttggagccga ggccaagtag
    24421 ctggtggtcg aggttcaggg tgatgtcatc actgcgctgc agctcgttac agtgctgggc
    24481 agcgtggatg ttttcctggg tatagtgcca ggcgctgaag ttaattgggc gctgcggaac
    24541 caccagcaga ccgttgccgt ggcggttagt cagtgccgtc cagcggacat gctgacggtt
    24601 gccgttgttc tgcgggaagg gatagttctc gaacatggca tcgacggtgc tgcgccagat
    24661 atcgatgatg ttagcctgct ggctgtcggc gtagttttcg cccggtccac gaccgtaata
    24721 cgccacctga tcgtattcgc cgttaatccc catggtgaaa ccgatgcacg gaatgatgtg
    24781 cggatagtcg ccgtaacgct cgccggaaag cgccacgtta acctggccat cggcagcgat
    24841 gcgccagatg taggtacagc gcatcccgaa gtcaaacacc ggcggtgcaa taactgtgcg
    24901 gctgatgatc aacacttcac catcgctctg ttctacggca aagtcgcgca gatgttcctg
    24961 catgatctgc aaatgattcg gctgccacaa cccttcgtac tcctgcttgt ggttgtcgat
    25021 catcggcttg aagaagttga tctttggctc gcgggtcagc agcgactcgc catttacctg
    25081 gcaggatgtc ggtttgccac tcatttttga gaaggtgatc gcgaagttgt agccgcgaac
    25141 ggtgcagctc aaacgatcgt cttccagcgt cagcggacgc gcattatttg gtgcgaaagg
    25201 cactggctgc gcggtgtttt ccttcagcgg gaactgataa gaggcgatag aatgcccggc
    25261 ttcgctgtag cgggtgcggg aatctttggt caccgtaatg ttgaggaacg cttcgcgggc
    25321 gtccagctgc ggcagcgtga tctgcaaggg ggcttcgctg ttcggcgcaa cgtcgcgcag
    25381 tttaatctgc tgcgtcgcga gcgtttcacc ttcggcgcgc acctctgcgt gcagggtgta
    25441 gtcatcaagc gtggtaaacc acagtttatt ttcgactttc aactcgccgc gagtcagcgc
    25501 gtggattttt accggcgcga taacctgttt gtactctttc aggcccgatc ccggcgtctg
    25561 atcggaatag atcaaaccat caagacagaa gttatagttg ttgggatagt cgccgccgaa
    25621 tttataccag acattgccgt ggtcgtcctg tgcctggatc ccgtggtcgc accactccca
    25681 gacataatga ccctgaatgc aatcgtgctt atagaagacg ttctggtact ccgtcagccc
    25741 gcccggtccg ttccccatcg catgagcata ttcacagatg atgcgcggct tcggatgcgg
    25801 gtattcacca aactcattca tcagcggcac gcgggtgtac atggtggaaa taatatcgac
    25861 cacttcagca tcgcgatctt cttcgtaatg aaccagtcgc gtgtcatcca gcgctttcgc
    25921 cgcatggtac atcgcgcgga tgttacagcc atagccggat tcattgccca gcgaccagat
    25981 gatgatcgac ggatggtttt tctgtgcgtg gatatggcga acaatgcgct cgacgtagac
    26041 tttttcccac tgcggatcgt cggtaatacg gctgatatcg ccgacattag caaagccgtg
    26101 cgattcgacg tcggtttccg ccatcacaaa caggccgtag atatcacaca gttcgtaaaa
    26161 acgcggatcg ttcgggtagt gagcggtacg cacggagttg atattgtgct gcttcatcaa
    26221 ctggagatct ttctcgacgc gatccattcc aacggcgcgg cctttgcgat gatcgttgtc
    26281 gtgacggttg acgccgtgca gcatcacata acggttattg atccagaaca gaccgtcgcg
    26341 cactttgata tcacggaagc caacgcgttg tggcaccact tccagaacgt tgccgttggc
    26401 gtctttcagc gtcatgacca gatggtaaag ataaggggat tctgctgacc attgctgcgg
    26461 ctgttcgaca gtaaaagcaa agctggcgct ggtcagtttt tcaattgcca aatgatcaat
    26521 ggcgctgctg tgcaccacgc gttcgccatc aaacagggta tattccagcg tcgtgacgac
    26581 aggggaggcg gcgagatttt ccagcaccac ttcgcaggaa agcgtggcat cgcaataggc
    26641 ttcgtcaaag tcggtacgca cagtgaaatc gttaatatgc gttaggtgtt ttccgaccag
    26701 ataaacatcg cggaagatcc ccgccgacca ccacatatcc tggtcttcca cgtaggtaga
    26761 gtccgcccac tgcatcacgc gcacacacaa caggttgtcg ccggttttaa ccatcgcgct
    26821 gatgtcaaac tctgcggtca ggcgactgcc cttgctgaaa cccacatact gaccgttaac
    26881 gtagacttca aaataggttt cgacgccgtc aaatttaatc agcgtctgtt taccctgcca
    26941 gccgtcgctg agggtgaaaa tacgttgata ggcaccggtt gggttatcgc tggggacaaa
    27001 cggcacatcg atggggaacg gaaaaccttc gtcggtatat tgcagtttgc cgtgaccttc
    27061 catttgccac atggcgggga cggtaatatg cccccagtca gccattaact cagaggtgaa
    27121 ggcttctggt acttgcagcg gatggtcaaa aaagtggaaa ttccactgac tgcttaaggg
    27181 cagaaacagg ctgctggttt cgcgggcaaa ggtacgcgct tgcgcaacag aatcatatga
    27241 aaaaaagtac gcacgcggcg caagtcggtt ttcgtgggtg agctgaatgt tttcccagcg
    27301 attcataggg cctcccggta gagagaaatc ggaaccattc atggtgatgg tagtttgtgt
    27361 aattgctggg gggttagtaa aatttttact aaaaagcagc aggtaaatgc atttacttta
    27421 actggatcac gaaaataaac ggcagcactg tccggcaaaa atgattgccg gacagtgtgt
    27481 aaggggattt aacgggtcgt gccgcgcagt tttaatttgc tgggaacgaa gactagcagc
    27541 ggcagcgcgc gaccatcgcg ggctttttca tacaccaggt taacgccctg acttcccatc
    27601 atctcggaat ggatgcgcac ggtggagagc ggcggaaagg taaatcgcgc ggtggggata
    27661 tcgttaacgc tgataagcga aatatcttgt gggatgttca ggccacgttc atgaattgcc
    27721 cgcagtacgc cgatagcaat ggaatcggaa gcaacaaaca gtgccttcgg atagtcttcc
    27781 cgcgccagca tttgttttgc cagttcataa cccgacgaac tggaaaaacc gccgcgccag
    27841 atatcctctt cgcgtaccac ttgtttcagt cggccatatt ccgcaaaggc gacctcacga
    27901 atatccgcct tgccaggctc atcttcaccg ccaataaaac caatacgatt aacgccctgg
    27961 ttgatataga agtcgatgat ttctttactg atgcgtgcca gatcgatatc caccgcatcg
    28021 taaccgctgc cgggttcgtg aaagtcgata aaacagatat tgtcggtcaa cgcgctggca
    28081 gcggcgcgca gggcgggcgt gggtttgccg acaattaaaa taccggtgac gtttttaatg
    28141 tctggtaatc cgttgtgttc ataacagttg gtcagttcga tcgccagctt ttcgcactga
    28201 gtttcaatgc cgtggcggat cgccagatag taaggatcgt tgatctccag ctcctgctgg
    28261 tagctgtaga tagccagaat atggtgttgg ttgactgcgc ctgtctgtag tttacgggcg
    28321 ctactggtct tgtactccag cttttcggcg atctcgagaa tgcgatgttt cgtctcttct
    28381 ttcacattca atgtcggatc gtcatttaag accctggata ctgtcgccag ggatacgcca
    28441 gcttcgattg cgatgtcttt taatgttgcc atgtttacct tttttacttt ccctgatgtg
    28501 cgcccccatt gtagaggaag tgcttatcgg gtcacctgtt ttcagtaaat aaagcgcgtt
    28561 tctttgacgc tgtaacggta acacagcggc ttaggaatgt gatcacgcct gcattctcca
    28621 tttttagcct acaggattga gaaaaacttt tctatagttt agtaaaattt tatccgaagg
    28681 gaggaagcaa tggaaaccgt ggcttacgct gattttgcac gtctggaaat gcgcgtcgga
    28741 aagattgtgg aagtgaaacg ccatgaaaac gccgacaagc tgtacatcgt acaggttgat
    28801 gtggggaaaa aacactgcaa accgtgacca gcctggtgcc gtactacagc gaagaggagc
    28861 tgatggggaa aacggtggtg gtcttatgca atctgcaaaa agcgaaaatg cgtggtgaaa
    28921 cgtcggaatg catgctgttg tgtgcggaaa ccgatgacgg cagtgaaagc gtgttgttaa
    28981 cgccggagcg gatgatgcct gcgggcgtac gcgtcgtata aaaagatcgg atggcgacgt
    29041 cgtatcgcca tccgatttga tattacgctt cttcgacact tactcgcatg gccgccagcg
    29101 ccttacgcgc cgctttgatc accagttcac actgttcaat ggtcagtgtc agtggcggtt
    29161 caatgcggat cgttttggcg ttattgagcg ttccggccac cagtacgcgc tggcggaaca
    29221 tctcgctggc aaagttatag ccgatttcgt tatcaacaaa ctcaatcgcc atcaacatcc
    29281 ctttaccacg cgcttcctgt accagatcgg gatattcccg cgccagttga cggaaaccgt
    29341 ccagcaacat atcgcctttt tgctcagcct gagccggtaa gttctgctcc agcaacacat
    29401 tgatggtcgc cagcgccgcc gcacaggcca gcgggttgcc gccaaaggtg gtggtatgca
    29461 ggaatgggtt gtcgaacaga actgaaaaca cctcttcagt ggcgatggtc gcgccaatcg
    29521 gcatcacgcc gccgccgagg gcttttgcca gacacagaat gtccggctga acgttctcat
    29581 gttcgcaggc aaacatcttg cccgtgcgcc ccatgcccgt ttgtacttca tcgaggatca
    29641 tcagtgcgcc gaactcatcg cacagcttac gcacggcggt gagatagccc ggcggcggca
    29701 gaattacgcc accttcgccc tgaatcggtt cgaggatcac cgcagccaca tcatcaccgg
    29761 tttttttgca ctcattaaga gccgtgcgca tggcttcgat attgccaaac ggcacatgac
    29821 ggaaccctgg cagcagtggc ataaacggtt tgcggaaggt cgatttcgcc gtggccgaca
    29881 gcgcgccaag tgatttaccg tggaacgcgc cgctggtggc aataaaagta aatttgccgc
    29941 gcggcgactg ataagccttc gccagcttca gcgccgcttc aacggactcg gtgccgctat
    30001 tacagaagaa gctgtatttc agtttaccgg gcgttagcgc agcaagggtt ttcgccagca
    30061 tagcccgtaa cggatcgagt agctcctggc tgtgcagtgg ttgtttcgca agttgattct
    30121 gtacggcgga aaccacaact ggattacggt gccccacgtt gaaaattcca aaacctccca
    30181 ggcagtcgat aaactcctgt ccctgggtgt cgacaagcgt atttaaacct cccgcttgcc
    30241 actctacggc tccgtaatcc ccgccggcgg taacagattt gcgatactct aaaaaccccg
    30301 gattgacatg ctctttgaag tattcaatca cctctcggtt aagtgctttc atctcctcat
    30361 gatccagcgt tcgcttctca atgagattca gggcgtgggc gctgcacgct aaagccgatg
    30421 cgctcgaagg taacctgttc aaaatatgct ccggaggctc gcgtatcaca tgataccgat
    30481 ttaagtattg cagggattgc gccactccgg ctgcgatcgc gaaaatcggg ataaacacaa
    30541 atgcggttat catttgtgca atatttaatg ctatccgtta acatttgttg ttattttgca
    30601 cggtttttgt cgggtatgga tttgatgatt gctgcgcaaa tgcacgtttt cagggcaata
    30661 attgcctgat ttttgggcaa gataattaac gttttcgtat agtaacagag tgaaagtatt
    30721 ttaaatcaaa gaattagatt tgtgctttaa tcgcaaattg cgatctaaat caaattaatc
    30781 ggttaaagat aaccgcagcg gggccgacat aaactctgac aagaagttaa caaccatata
    30841 acctgcacag gacgcgaaca tgtcttctca tccgtatgtc acccagcaaa ataccccgct
    30901 ggcggacgat accactctga tgtccactac cgatctgcaa agctatatca ctcatgctaa
    30961 tgacactttt gtgcaggtga gcggctttac cttgcaagag ttacaagggc agccgcacaa
    31021 catggtgcgt cacccggata tgccaaaagc ggcgtttgcg gatatgtggt tcaccctgaa
    31081 aaaaggggag ccctggagcg gcatcgtgaa aaatcgccgc aaaaatggcg accattattg
    31141 ggtgcgggcc aatgcggtac cgatggtgcg cgagggaaaa atcagtggct atatgtcgat
    31201 tcgtacccgg gcgacggatg aagagattgc ggcggtggag ccgctgtaca aagcgttgaa
    31261 ctccggacgt accagtaagc gtattcataa aggcctggtg gtgcgtaaag gctggctggg
    31321 taaactgcct tcattaccgc ttcgctggcg gacgcgtgga gtgatgaccc tgatgtttat
    31381 cttgctggcg gccatgcttt ggtttgttgc tgccccggtg gtgacgtatt tcctctgtgt
    31441 gttagtggta ttgttggcaa gcgcttgttt tgaatggcag attgtgcgcc cgatagaaaa
    31501 tgtcgcccgt caggcactga aggtggcgac cggagagcgt aatagtgttg agcacctgaa
    31561 tcgcagcgat gagctggggc tgacattacg cgcggtaggg cagcttggcc tgatgtgccg
    31621 ttggctaatt aacgatgtct caagccaggt gtccagtgtc agaaatggca gtgagacgct
    31681 ggcgaaaggc accgatgaac tgaacgaaca tacccagcag acagttgata acgttcagca
    31741 aacggtggcg accatgaacc aaatggcggc gtcggtgaaa cagaactctg ccacggcgtc
    31801 ggctgccgat aaactttcta tcaccgccag taatgcggca gtgcagggtg gggaggcgat
    31861 gaccacggtg atcaagacaa tggacgatat cgccgacagt acctagcgca ttggcaccat
    31921 tacttcgctg attaacgata ttgcgtttca gaccaatatt ctggccctga atgcggcggt
    31981 ggaagcggcg cgtgccggcg aacagggcaa aggttttgca gtggtggcag gggaagtgcg
    32041 tcatttagcc agccgcagcg ctaatgctgc caacgatatt cgcaagctga ttgatgccag
    32101 tgctgataag gtgcaatccg gttcgcagca ggtacacgcc gccggacgga cgatggaaga
    32161 tattgtggca caggtgaaaa acgtcaccca gttgatcgcc cagattagcc attcaacgct
    32221 ggaacaggcc gatgggcttt ccagcctgac ccgtgcagtg gatgagctta acctgatcac
    32281 ccagaaaaat gccgagctgg tggaagagag tgcgcaggtg tcggcgatgg tgaaacaccg
    32341 cgccagccga ctggaagacg cggtgacggt gctgcattaa tcggtatgcc ggatccggcg
    32401 gttggagcac aatgcctgat gcgatgctgg cgcatcttat caggcctgcg gggtgtgtag
    32461 cgggtgtagg ccggataagg cgcgtcaagc gtcgcatccg gcaattgcac cgcgccactg
    32521 gcggatgcgg cgattaatcc agttgcgtaa tatcaaaagc ggcgcggtcg atcaccgcaa
    32581 tgatcttttt tatttgtgca tcactgatat cgctctggtt gacgcgtaaa tccagcaccg
    32641 ctttaaaatt atccagcgcc cgcttcattt gcgggttctg gcgcagcgcc gcgccaacgc
    32701 aacgcgcttt gatgcgttct tcaatcatct ccacttgttc gcggttttct tccagccact
    32761 gcgcaccttg ttcggtcagc gcaatctgct tcttaccacc ttcctcttcg cggatggtaa
    32821 tcagcgactg ctcctgcaga aaatccagcg tcgggtagat gacgcccggg cttggggtgt
    32881 aattcccctg ggttagattc tcaatcgctt taatcaattc gtaaccgtgg ctgtcatcgc
    32941 gcgagagaat atccagaatc accagacgca attcaccgtg accaaagaaa cgttgccgac
    33001 gaccgccgcc gcgaccgtgg cgaccaccgc aacattgacc gtgttcatgc tggtgaccgt
    33061 gtccgcagtg ctcgtgttct gacttctcac ctttgcagca gccctcatgc cgtggctggc
    33121 cttcatgttt acaacaccct tcgtgatgat ggctcatatc agcctccttt tgcttgatta
    33181 gatatatcta atttatatct gatgtgagca ttactgcaag tacaaaaatg atttttaaac
    33241 gtggaagcac ctcacatcta ggccggataa gacgttcacg ccgcatccgg cagcattgct
    33301 atggcactga atgccttcac atacttcaga catatcagtt ctacaaatcg cttgcattta
    33361 tcatgattaa caatcattat catttgcgaa ttttatttag atatatctga ttaccatcac
    33421 gaaggcgata acaatgaata acaccccccg ctacccgcag cgcgttcgca atgatctgcg
    33481 cttccgtgaa ctgactgtgc tacgcgttga gcgcatcagc gccggttttc agcgcattgt
    33541 ccttggcggc gaggcgttgg acggttttac atcgcgcggc tttgacgatc acagcaaact
    33601 cttctttcct caacctgacg ctcactttgt gccgccaacg gtaacggaag agagcatcgt
    33661 ctggccggaa ggaccacgcc caccgtcgcg tgactatacg ccactgtatg acgaactacg
    33721 ccatgaactg gcgatcgatt tctttattca cgacggtggg gtcgccagcg gctgggcgat
    33781 aaacttacgg tggcaggtcc gcgcggttcg ctggtggtgc cggaagatta cgcgtatcag
    33841 ctgtatgtct gcgatgaatc cggaatgccc gcactgcgcc gccgcctgga aacgttgagc
    33901 aaacttgccg ttaaaccgca agttagtgcg ctggttagcg tgcgggataa cgcctgtcag
    33961 gattatctcg cgcaccttga tggttttaat atcgaatggc tggcacatga tgagcaggcg
    34021 gtagatgcgc gtctggcgca gatgcaaatc cctgccgatg attacttcat ctggataacc
    34081 ggcgaaggta aagtcgttaa gaatttaagc cgccgctttg aagcggaaca gtatgaccca
    34141 cagcgggtac gtgcagcggc ttactggcac gcaaagtaac tgatatataa aaagaaaaag
    34201 gctgacgatt tctcgtcagc ctttgctata tctggtggcc cctgctggac ttgaaccagc
    34261 gaccaagcga ttatgagtcg cctgctctaa ccactgagct aaggggccat ggtagcggat
    34321 tataaagtaa ctccgtgtcg caatccagcc attaccgcgc gcctgctgtt tttataaaca
    34381 atgtattttc aatccgttat acttttcctg gtgatgttag aaaggagtaa atatggttga
    34441 ggatattttg gctccagggt tacgggtcgt gttttgcggt atcaaccctg ggctttcatc
    34501 cgccgggact ggttttccct ttgctcatcc ggcaaatcgc ttctggaagg tgatatatca
    34561 ggccgggttt accgaccgtc agttgaagcc gcaggaggca cagcatctgc tggattatcg
    34621 ttgtggcatc accaaactgg tagaccgtcc aacggtgcaa gccaatgaag tttcaaagca
    34681 ggagctgcac gcaggtgggc gtaagctgat tgaaaaaatt gaagattatc agccgcaggc
    34741 gttggcgatt ctgggcaaac aagcatatga acagggattc agctagcgcg gtgcacagtg
    34801 ggggaaacaa acgctcacca ttggttcgac gcagatttgg gtgctgccaa atcccagcgg
    34861 tttaagtcgc gtttcactgg agaaactggt tgaagcgtat cgcgagctgg accaggcgct
    34921 ggtagtgcgt gggcgataa
//
LOCUS       DAEEUE010000035        34447 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.035, whole genome shotgun sequence.
ACCESSION   DAEEUE010000035 DAEEUE010000000
VERSION     DAEEUE010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34447
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.035"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..334)
                     /locus_tag="KLK84_001830"
     CDS             complement(<1..334)
                     /locus_tag="KLK84_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009177.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVTADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQK"
     gene            704..1654
                     /gene="corA"
                     /locus_tag="KLK84_001831"
     CDS             704..1654
                     /gene="corA"
                     /locus_tag="KLK84_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HBE2009178.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1695..2075)
                     /locus_tag="KLK84_001832"
     CDS             complement(1695..2075)
                     /locus_tag="KLK84_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HBE2009179.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2089..2505)
                     /locus_tag="KLK84_001833"
     CDS             complement(2089..2505)
                     /locus_tag="KLK84_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009180.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2564..3454)
                     /gene="rarD"
                     /locus_tag="KLK84_001834"
     CDS             complement(2564..3454)
                     /gene="rarD"
                     /locus_tag="KLK84_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HBE2009181.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3506..3973)
                     /locus_tag="KLK84_001835"
     CDS             complement(3506..3973)
                     /locus_tag="KLK84_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HBE2009182.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4138..5007
                     /gene="pldA"
                     /locus_tag="KLK84_001836"
     CDS             4138..5007
                     /gene="pldA"
                     /locus_tag="KLK84_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HBE2009183.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5140..6969
                     /gene="recQ"
                     /locus_tag="KLK84_001837"
     CDS             5140..6969
                     /gene="recQ"
                     /locus_tag="KLK84_001837"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBE2009184.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7033..7653
                     /gene="rhtC"
                     /locus_tag="KLK84_001838"
     CDS             7033..7653
                     /gene="rhtC"
                     /locus_tag="KLK84_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HBE2009185.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7715..8335)
                     /gene="rhtB"
                     /locus_tag="KLK84_001839"
     CDS             complement(7715..8335)
                     /gene="rhtB"
                     /locus_tag="KLK84_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HBE2009186.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8446..9468
                     /gene="pldB"
                     /locus_tag="KLK84_001840"
     CDS             8446..9468
                     /gene="pldB"
                     /locus_tag="KLK84_001840"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HBE2009187.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGLWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9476..10276
                     /gene="yigL"
                     /locus_tag="KLK84_001841"
     CDS             9476..10276
                     /gene="yigL"
                     /locus_tag="KLK84_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HBE2009188.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10352..11251
                     /gene="bioP"
                     /locus_tag="KLK84_001842"
     CDS             10352..11251
                     /gene="bioP"
                     /locus_tag="KLK84_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HBE2009189.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11139..12092)
                     /gene="metR"
                     /locus_tag="KLK84_001843"
     CDS             complement(11139..12092)
                     /gene="metR"
                     /locus_tag="KLK84_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HBE2009190.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12328..14589
                     /gene="metE"
                     /locus_tag="KLK84_001844"
     CDS             12328..14589
                     /gene="metE"
                     /locus_tag="KLK84_001844"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBE2009191.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14628..15443)
                     /locus_tag="KLK84_001845"
     CDS             complement(14628..15443)
                     /locus_tag="KLK84_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBE2009192.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15705..16466
                     /gene="udp"
                     /locus_tag="KLK84_001846"
     CDS             15705..16466
                     /gene="udp"
                     /locus_tag="KLK84_001846"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HBE2009193.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16607..18034
                     /gene="rmuC"
                     /locus_tag="KLK84_001847"
     CDS             16607..18034
                     /gene="rmuC"
                     /locus_tag="KLK84_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HBE2009194.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18129..18884
                     /gene="ubiE"
                     /locus_tag="KLK84_001848"
     CDS             18129..18884
                     /gene="ubiE"
                     /locus_tag="KLK84_001848"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HBE2009195.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18898..19503
                     /gene="ubiJ"
                     /locus_tag="KLK84_001849"
     CDS             18898..19503
                     /gene="ubiJ"
                     /locus_tag="KLK84_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HBE2009196.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19500..21140
                     /gene="ubiB"
                     /locus_tag="KLK84_001850"
     CDS             19500..21140
                     /gene="ubiB"
                     /locus_tag="KLK84_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HBE2009197.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21219..21488
                     /gene="tatA"
                     /locus_tag="KLK84_001851"
     CDS             21219..21488
                     /gene="tatA"
                     /locus_tag="KLK84_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HBE2009198.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21492..22007
                     /gene="tatB"
                     /locus_tag="KLK84_001852"
     CDS             21492..22007
                     /gene="tatB"
                     /locus_tag="KLK84_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HBE2009199.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22010..22786
                     /gene="tatC"
                     /locus_tag="KLK84_001853"
     CDS             22010..22786
                     /gene="tatC"
                     /locus_tag="KLK84_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HBE2009200.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22828..23610
                     /gene="tatD"
                     /locus_tag="KLK84_001854"
     CDS             22828..23610
                     /gene="tatD"
                     /locus_tag="KLK84_001854"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HBE2009201.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23607..24095)
                     /gene="rfaH"
                     /locus_tag="KLK84_001855"
     CDS             complement(23607..24095)
                     /gene="rfaH"
                     /locus_tag="KLK84_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HBE2009202.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24262..25755
                     /gene="ubiD"
                     /locus_tag="KLK84_001856"
     CDS             24262..25755
                     /gene="ubiD"
                     /locus_tag="KLK84_001856"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HBE2009203.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGEHFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25801..26502
                     /gene="fre"
                     /locus_tag="KLK84_001857"
     CDS             25801..26502
                     /gene="fre"
                     /locus_tag="KLK84_001857"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HBE2009204.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26694..27857)
                     /gene="fadA"
                     /locus_tag="KLK84_001858"
     CDS             complement(26694..27857)
                     /gene="fadA"
                     /locus_tag="KLK84_001858"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HBE2009205.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27867..30056)
                     /gene="fadB"
                     /locus_tag="KLK84_001859"
     CDS             complement(27867..30056)
                     /gene="fadB"
                     /locus_tag="KLK84_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HBE2009206.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30246..31577
                     /gene="pepQ"
                     /locus_tag="KLK84_001860"
     CDS             30246..31577
                     /gene="pepQ"
                     /locus_tag="KLK84_001860"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HBE2009207.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31577..32191
                     /locus_tag="KLK84_001861"
     CDS             31577..32191
                     /locus_tag="KLK84_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HBE2009208.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32230..33681
                     /gene="trkH"
                     /locus_tag="KLK84_001862"
     CDS             32230..33681
                     /gene="trkH"
                     /locus_tag="KLK84_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HBE2009209.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33693..34238
                     /gene="hemG"
                     /locus_tag="KLK84_001863"
     CDS             33693..34238
                     /gene="hemG"
                     /locus_tag="KLK84_001863"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HBE2009210.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8103 a         8704 c         9305 g         8335 t
ORIGIN      
        1 ccttctgctg accgttttcg atgtacaaac cgagcggcgc atagctttca tcatagatgc
       61 cgccgttcat cgccatctgc acctgaccct gactattaat atccgccagc agagcatgta
      121 acgttcccca cgcttcgcca ttggcttttt gccagtacat tttcacccgc tctgtttgag
      181 gattaacggt atacgcctgt acggtcagcg tcggatctga gagtgcgcaa tcatcagcgg
      241 taacagcaaa caagggaagt aaggtgaggg tgagaaaaat ccgtttgaga ttcaaggtga
      301 tcattccttt accaatgagt agccgatgcg ccattatagg tcctagatgt gggatttttt
      361 tatcctgtta gcgaccttga cgagtaccaa aaagcgcgaa gttcaactat tgttctgtgg
      421 tgttctgttg cgtgttgacg gcaaaatttt gctggcgtaa catgcgcgca cgatcactct
      481 aagaggacat tcgccttgga cacacccagt agatactggc tcactatcct gtcatccagg
      541 atcaactcct aaggctatcc ctttttgctg atagccttag cggttgtcag cgacctcaat
      601 ttttcccgtc gcgctgagtc aggctgttta atggtctgaa acccaatttg tttctgtgtg
      661 cccaccgaac tgtccgatat tttaagcatt gggagtcccg gtcatgctga gcgcatttca
      721 actggaaaat aaccgactga cccggctgga agtcgaagag tcacaacccc ttgtaaatgc
      781 agtatggatt gatcttgtcg aaccggacga cgacgagcga ctgcgcgtac aatctgaact
      841 tggccagagc ctggcaaccc gcccggaact ggaagacatc gaagcatcgg cacgtttctt
      901 tgaagacgac gacggcctgc atattcactc cttcttcttc tttgaagatg cggaagatca
      961 cgccggtaac tccactgtgg catttaccat ccgtgatggt cgtctgttta ctctgcgtga
     1021 gcgtgaactg cccgcttttc gtctgtatcg tatgcgtgcc cgtagccagt cgatggtaga
     1081 cggtaacgcc tacgagttgc tgctggatct gttcgaaacc aaaatcgaac agttggcaga
     1141 tgaaattgaa aatatctata gcgacctgga gcagttgagc cgggtgatta tggaagggca
     1201 tcagggcgat gagtacgacg aggcgctctc cacgctggcg gaactggaag atatcggctg
     1261 gaaagttcgc ctgtgtctga tggataccca gcgcgctctc aacttcctgg tgcgtaaagc
     1321 gcgtttaccg ggtgggcaac tggagcaggc gcgtgaaatc ctgcgcgata tcgaatccct
     1381 gctgccgcat aacgaatccc tgttccagaa ggtgaacttc ctgatgcagg cggcaatggg
     1441 ttttatcaac atcgagcaga accgcatcat caaaattttc tcggtggtat ccgtcgtgtt
     1501 cctgccgcca acgctggttg cttccagcta cgggatgaac tttgagttta tgccagaact
     1561 gaagtggagt ttcggctacc ctggcgcgat tatctttatg atcctcgcgg gcctggcacc
     1621 gtatctgtac tttaagcgga agaactggtt gtaaaaagaa ggcggtggct tagcctggct
     1681 aagccacctg ttattcaaag gctccaggta tttaaccctt ttacctcttt ctcataaaac
     1741 catttgttcg tgttaacagc aacataggct gctacggcaa ttcccagaat gttaacgcca
     1801 attaatgcac caacaaatag agatagaatg ccaagtaata gaactataat tgcttttttc
     1861 cataagccca gaacaaataa atagatccag gaacagaaga aagcaatgaa attcatttga
     1921 atagttaatc gttgtcttac ttttaatgct ttaaatgctg ctttatattc tggtgttgct
     1981 ccccagaatc caggaaagcc atgttgatca taaaaattaa atcggtattt ccatttttca
     2041 cttaatgaat catcgttcat atattcctta ctcataaata ctccataact attgttttga
     2101 tgaatcagta ggtgcaagca ttagcatact gaaagtggaa aaataacaaa tcaaaaaaat
     2161 catcgaacca ttgcctgaac aggcaaaatc ttcggctatc attgtgatga tagagatgat
     2221 atatactgct aatgtaccaa aaacataaat ttttatatat atgaaaccac tatcacggag
     2281 tcgttggcaa ttcatgttga tgacgagata atggagtgcg atggtagaga ctataacaag
     2341 aaaacctgct tccccatcgt taaaaaagat aataagaaag gcaaaaatga aatttattaa
     2401 aataaataaa aatatatatc ggtgtctgga aatcttaccg ttagatgttg ggataaacat
     2461 acgtaacata aattttacat ccttgtgtga gtctcctgtc agcatggcaa tatgcccact
     2521 ctcatacaag agtcggcata ttttttcaga atatatttat tttttatttg gacgttctac
     2581 gctgcgtata aatcgcgtcc atcacaaaaa ttgccagcgc cacccaaata aaggtgaaag
     2641 tcaccatctt atcggcaccc ggtttttcac cataaaacgt cacagccagc aggaacatca
     2701 gcgtcgggcc aatgtactga aaaaagccta acgttgagag acgcaggcgc gtggcagcgg
     2761 cagtaaaaca caacagcggt acggtagtga caataccggc ggcgatcagc agtaaattca
     2821 gcgacatcgg gttttgcccc atatggctgg ttgaactgtc ggcaatagca aacaggtaaa
     2881 tcgccgccac gggcagcagc cacatggttt caattaacat gccggtttgt gcttctaccg
     2941 caatcttctt gcgcaccagg ccgtagaagg caaaactaaa tgccagcccc agcgcgataa
     3001 taggtagcga accaaaagtc cacagctgga ccaacacgcc acatatcgcc agaatcaccg
     3061 ccagccattg catccggcgg aatcgctcgc cgaggaaaat catccccagc acaatgttca
     3121 ccagcgggtt aataaagtaa ccaaggctcg cttccagcat atggtgattg ttcaccgccc
     3181 agataaacag tagccagttg ccaccaatca gtacggcaga gactgccagc ataaaaattt
     3241 tctgtggcgt ctgaatcagc gtttttaaat aggaccactg gcggcaaatg ctcatcagca
     3301 ccaccataaa gaaaaacgac cagatcacgc gatgcgtcag gatctcatcg gcgggcacgt
     3361 agtaaatcag cttgaagtat gctggcgcaa taccccaaat aaaataagcg gcaagagcga
     3421 gtaatacgcc ctgccgcgtt tgttttgcat ccatcgggaa tactcatttt taattggtaa
     3481 cagcagttta cctgctttta tgtcttcaac ccaccatata ggtggcggtg gcactggcaa
     3541 tataaagctg ttcttcattg tgtaattcaa cgcgggcgac ggcgacttta ttgcctgcac
     3601 gcaacaggct actggtagca gtaaaacgct cgcccctgcc tgggcgcaga taatcaacgc
     3661 gaagatcaat ggtccccatc cgcgatagcc gctggcgtag ttcatcttca ctgatggttt
     3721 cgtggcgggt taaggtactt cccacgcaca ccaggccggc ggcgacatcc agcgccgacg
     3781 caatgactcc gccgtgcaaa atgctttgcg cccagttacc caccatcatc ggctgatttt
     3841 taaaggccag ctgtgcgaac tctttttcgt aacgctccag ttccatcccc aatgcgcggt
     3901 taaatggcat gtgataaaca aacatctcac ccactaattt cagggcttgt tcagcggtca
     3961 gtacggcaga catatcatcc ttacacttca ttggttaatg aaatgttgat tttatgcttc
     4021 tttgttgttg gtttctactt taggaaggga taactaacgg caatggagtt aagtatgtaa
     4081 aatagcccgc agaaaaacat tcaccttatc aataattcgt tacggagaac acgaccgatg
     4141 cggactctgc agggctggtt gttgccggtg tttatgttgc ctatggcagt atatgcacaa
     4201 gaggcaacgg tgaaagaggt gcatgacgcg ccagcggtgc gtggcagtat tatcgccaat
     4261 atgctgcagg agcatgacaa tccgttcacg ctctatcctt atgacaccaa ctacctcatc
     4321 tacacccaaa ccagcgatct gaataaagaa gcgattgcca gttacgactg ggcggaaaat
     4381 gcgcgtaagg atgaagtaaa gtttcagttg agcctggcat ttccgctgtg gcgtgggatt
     4441 ttaggcccaa actcggtgtt gggtgcgtct tatacgcaaa aatcctggtg gcaactgtcc
     4501 aatagcgaag agtcttcacc gtttcgtgaa accaactacg aaccgcaatt gttcctcggt
     4561 tttgccaccg attaccgttt tgcaggttgg acgctgcgcg atgtggagat ggggtataac
     4621 cacgactcta acgggcgttc agacccgacc tcccgcagct ggaaccgcct ttatactcgc
     4681 ctgatggcag aaaacggtaa ctggctggta gaagtgaagc cgtggtatgt ggtgggtaat
     4741 actgacgata acccggatat caccaaatat atgggttact accagcttaa aatcggctat
     4801 cacctcggtg atgcggtgct cagtgcgaaa ggacagtaca actggaacac cggctacggc
     4861 ggcgcggagt taggcttaag ttacccgatc accaaacatg tgcgccttta tactcaggtt
     4921 tacagcggct atggcgaatc gctcatcgac tataacttca accagacccg tgtcggtgta
     4981 ggggttatgc taaacgactt gttttgatga acggttgagt ggttggcaaa tctggaatcc
     5041 agcatccagg attaccctct cagagactaa aagcattgca gtttctcgcg caggcgctga
     5101 aaatagcgcc tgtttttatt tcaggcaatc ggggtgaatg tggcgcaggc ggaagtgttg
     5161 aatctggagt ccggagctaa acaggtttta caagaaacct ttggctacca acagtttcgc
     5221 cccggccagg aagaaattat cgacactgtg ctttccggtc gcgattgcct ggttgttatg
     5281 cccactggtg gcggaaaatc cctttgctat caaattcctg ccttattgct aaacggcctt
     5341 accgtggttg tttcaccgct gatttcgttg atgaaagatc aggtggatca actacaggcc
     5401 aacggcgtgg cggcggcgtg ccttaactcg acgcaaaccc gcgaacagca acttgaagtg
     5461 atgacaggct gccgcaccgg gcaaattcgc ttactgtata tcgccccgga acgcctgatg
     5521 ctggataact ttcttgagca tctggcgcac tggaatccgg tgttattagc cgtcgatgaa
     5581 gcgcactgta tctcccaatg gggccacgat ttccgcccgg aatatgccgc gctcggtcag
     5641 ttgcgccagc ggttcccgac gctgccgttt atggcgctga ccgccacagc cgacgacacc
     5701 acgcgccagg atatcgtgcg cctgctgggg ctgaacgatc cgctgattca aatcagcagt
     5761 tttgaccgtc cgaatattcg ctacatgctg atggagaaat tcaaaccgct cgatcagttg
     5821 atgcgctacg tgcaggaaca gcgcggtaag tctggcatta tctactgcaa cagccgggcg
     5881 aaagtagaag acaccgctgc gcgcctgcaa agcaggggta ttagcgcggc ggcctatcat
     5941 gccgggctgg aaaataatgt ccgcgccgac gtgcaggaga aattccagcg cgatgatctg
     6001 caaattgtgg tggcgacggt ggcgttcggc atgggcatca ataaaccaaa cgttcgcttc
     6061 gtggtccact ttgatattcc gcgcaatatc gaatcctatt atcaggaaac cggtcgcgcc
     6121 gggcgtgatg gcctgcctgc ggaagcgatg ctgttttacg atccggctga tatggcgtgg
     6181 ctgcgccgtt gtctggaaga gaagccgcag gggcagttgc aggatatcga gcgccacaaa
     6241 ctcaatgcga tgggcgcgtt tgccgaagcg caaacttgcc gtcgtctggt attgctgaac
     6301 tattttggcg aagggcgtca ggagccgtgc gggaactgcg atatctgcct cgatccgccg
     6361 aaacagtacg acggttcaac cgatgctcag attgcccttt ccaccattgg tcgtgtgaat
     6421 cagcggtttg ggatgggtta tgtggtggaa gtgattcgtg gtgctaataa ccagcgtatc
     6481 cgcgactatg gtcatgacaa actgaaagtc tatggtatgg gccgtgataa aagccatgaa
     6541 cattgggtga gcgtgatccg ccagctgatt catctcggcc tggtgacgca aaatattgcc
     6601 cagcattctg ccctacaact gacagaggcc gcgcgcccgg tgctgcgcgg cgaatcctct
     6661 ttgcaacttg ccgtgccgcg tatcgtggcg ctcaaaccga aagcgatgca gaaatcgttc
     6721 ggcggcaact atgatcgcaa actgttcgcc aaattacgca aactgcgtaa atcgattgcc
     6781 gatgaaagta atgtcccgcc gtacgtggtg tttaacgacg caaccttgat tgagatggct
     6841 gaacagatgc cgatcaccgc cagcgaaatg ctcagcgtta acggcgttgg gatgcgcaag
     6901 ctggaacgct ttggtaaacc gtttatggcg ctgattcgtg cgcatgtcga tggcgacgac
     6961 gaagagtagt cagcagcata aaaaagtgcc agtatgaaga ctccgtaaac gtttcccccg
     7021 cgagtcaaat gtatgttgat gttatttctc accgtcgcca tggtgcacat tgtggcgctt
     7081 atgagccccg gtcccgattt cttttttgtc tctcagaccg ctgtcagtcg ttcccgtaaa
     7141 gaagcgatga tgggcgtgct gggcattacc tgcggcgtaa tggtttgggc tgggattgcg
     7201 ttgcttggcc tgcatttgat tatcgaaaaa atggcctggc tgcatacgct gattatggta
     7261 ggcggtggcc tgtatctctg ctggatgggt taccagatgc tacgcggtgc actgaaaaaa
     7321 gaggctgtat ctgcgcctgc accacaggtt gagctggcga aaagcgggcg cagtttcctg
     7381 aaaggcttac tgaccaatct cgccaatccg aaagcgatta tctattttgg ttcggtattc
     7441 tcattgtttg tcggcgataa cgttggcact accgagcgct ggggcatttt cgcgttgatt
     7501 attgtcgaaa cactggcgtg gtttaccgtc gttgccagtc tgtttgccct gccgcaaatg
     7561 cgccgtggtt atcaacgtct ggcgaagtgg attgatggtt ttgccggggc gttatttgcc
     7621 ggatttggca ttcatttgat tatttcgcgg tgatgccaga cgcgtcttca gagtaagtcg
     7681 gataaggcgt ttacgccgca tccgacatta tttttcacgc atgcctcgcc gatgctaaca
     7741 gcgctcccac cagcataaac aacgagccga aaatcttatt cagcgccttc atctgctttg
     7801 gtcctttaat ccatagagca atcctttgag caagggtggc gtaaccgatc atcacaataa
     7861 tatcgaccac aatagtggtg acgccgagca cgatatactg catcagttgc ggctgttgcg
     7921 gcatgatgaa ttgcggaaat agcgccgcca gaaacacaat acttttggga ttggtgagat
     7981 tcacaaaaac tgcgcgctgg aacaaatgtc gacgcgattg agtagaggcc agcgatttaa
     8041 gatcaattgc gccagcggcg cgccactgct ggattcccag ccaaatcaag taagccgcgc
     8101 ctgcccactt taacacttca aacgcaatca ctgagcggga aaatagcgtc cccaacccca
     8161 cgccaaccag cacaatatga atcgccagtc cggtctgaag cccagcaata gacgccaccg
     8221 cgccgcgata accgtggttg agcgaggtgg tcatagtgtt gattgcacca gagcctggcg
     8281 acaggcttaa aatgatcgat gtcagcaggt aggcaaacca ccattctaag gtcatgatga
     8341 actcccggtg tgtctatttt tgtgccacaa tacgctactg tcgcagcgtt gtgtcaggca
     8401 cgcttaaaaa aacgatttta cgtggtttaa gaggcagatt acccgatgtt tcagcagcaa
     8461 aaagactggg aaacaagaga aaacgcgttt gctgctttta ccatgggacc gctgactgat
     8521 ttctggcgtc agcgtgatga agcagagttt actggtgtgg atgacattcc ggtgcgcttt
     8581 gtccgttttc gcgcacagca ccatgaccgg gtggtagtca tctgcccggg gcgtattgag
     8641 agctacgtaa aatatgcgga actggcctac gacctgttcc atttggggtt tgatgtctta
     8701 atcatcgacc atcgcgggca gggacgttcc ggtcgcctgt tagccgatcc gcatctcggg
     8761 catgttaatc gctttaatga ttatgttgat gatctggcgg cattctggca gcaggaggtt
     8821 cagcccggtc tgtggcgtaa acgctatata ctggcacatt cgatgggcgg tgcgatctcc
     8881 acattatttc tgcaacgcca tccaggtgta tgtgacgcca ttgcgctaac tgcgccaatg
     8941 tttgggatcg tgattcgtat gccgtcattt atggcacggc agatcctcaa ctgggccgaa
     9001 gcgcatccac gtttccgtga tggctatgca ataggcaccg ggcgctggcg cgcgttgccg
     9061 tttgctatca acgtactgac ccacagcaga cagcgatatc gacgtaactt acgcttctat
     9121 gctgatgacc caacgattcg cgtcggtggg ccgacctacc attgggtacg cgaaagtatt
     9181 ctggctggcg aacaggtgtt agccggtgcg ggtgatgacg ccacgcaaac gcttctctta
     9241 caagctgaag aggaacgcgt ggtggataac cgcatgcacg accgtttttg tgaactccgc
     9301 accgccgcgg gccatcctgt cgaaggagga cggccgttgg taataaaagg tgcttaccat
     9361 gagatccttt ttgaaaagga cgcaatgcgc tcagtcgcgc tccacgccat cgttgatttt
     9421 ttcaacaggc ataactcacc cagcggaaac cgctctacag aggtttaaat ttcttatgta
     9481 ccaggttgtt gcgtctgatt tagatggcac gttactttct cccgaccata cgttatcccc
     9541 ttacgccaaa gaaactctga agctgctcac cgcgcgcggc atcaactttg tgtttgcgac
     9601 cggtcgtcac cacgttgatg tggggcaaat tcgcgataat ctggagatta agtcttacat
     9661 gattacctcc aatggtgcgc gcgttcacga tctggatggc aatctgattt ttgctcataa
     9721 cctggatcgc gacattgcca gcgatctgtt tggcgtagtc aacgacaatc cggacatcat
     9781 tactaacgtt tatcgcgacg acgaatggtt tatgaatcgc catcgcccgg aagagatgcg
     9841 cttttttaaa gaagcggtgt tcaaatatgc gctgtatgag ccgggattac tggagccgga
     9901 aggcgtcagc aaagtgtttt tcacctgcga ttcccatgaa caactgctgc cgctggagca
     9961 ggcgattaac gctcgctggg gcgatcgcgt caacgtcagt ttctctacct taacctgtct
    10021 ggaagtgatg gcgggcggcg tgtcgaaagg ccatgcgctg gaagcagtgg cgaagaaact
    10081 gggctacagc ctgaaggatt gtattgcgtt tggtgacggg atgaacgacg ccgaaatgct
    10141 gtcgatggcg gggaaaggct gcattatggg cagtgcgcac cagcgtctga aagatctgca
    10201 tccggagctg gaagttattg gtactaatgc cgacgacgcg gtgccgcatt atctgcgtaa
    10261 actctattta tcgtaatcgt tctttatttg gtcagttgtc aacctgatac ttcgctacaa
    10321 tggatactcg ttaatcaaag agttttccat tgtggcgtta cttatcatca ccacgattct
    10381 gtgggccttc tcctttagct tttatggcga gtaccttgcg gggcacgtcg atagctattt
    10441 tgcggtgctg gtgcgcgttg gcttggcggc actcgttttt ctgccgtttc tgcgtacccg
    10501 tagcaatagc ctgaaaacgg tcggcctgta tatgctggtg ggcgcgatgc agctcggcgt
    10561 gatgtatatg ctgagtttcc gcgcttatct ctacctgacg gtctccgaat tgttgctgtt
    10621 caccgtactg acgccgctct acatcacgct gatttatgac atcatgagta agcgccgtct
    10681 gcgctggggc tatgctttta gcgccttgct ggcggtgatt ggtgccggga ttattcgcta
    10741 tgatcaggtc accgaccatt tctggactgg cttgttgctg gtgcaactct ccaatatcac
    10801 ttttgccatc ggcatggtgg gttacaaacg cctgatggaa actcgcccga tgccgcagta
    10861 taacgccttt gcgtggttct atcttggcgc gttcctggtg gcggtaattg catggttctt
    10921 gctgggaaat gcgcagaaaa tgccgcaaac cacgttgcaa tggggcattc tggtgtttct
    10981 tggcgtggtg gcttccggga ttggctactt tatgtggaac tacggcgcga cgcaggtgga
    11041 cgccggaacg ctgggcatta tgaataatat gcacgttccg gcagggctgc tggtaaacct
    11101 ggctatctgg caccaacagc cgcactggcc aacgtttatt acaggcgcgc tggtgatcct
    11161 ggcctcactg tgggtgcatc gtaagtgggt cgctccgcgc tcttcacaaa cggcagatga
    11221 tcgcaggcgt gattgcgcgc tgagcgaata aacgcttccg taactggctg acgctgctcg
    11281 ccatcgcgca cggcggcgta cagtcggctc cacaagcctt cgcccagggt tttggtcacc
    11341 accagaccct ggcgctcaaa actctctact acccaatgcg gtagcgcggc aatacccatc
    11401 cgcgcggcaa ccatttgaat caacaataag gtgttatcga cgcttttcag tgacgggctg
    11461 acgcctgccg gctgaagaaa atgccgccag acatccagtc gactacgctg caccggataa
    11521 attaatagcg tctcgctggc gagatcttcc ggtgtaatac gcgtttttgc cgccagtgga
    11581 tggtcaggtg ctaacaccag gcgtacttca taatcgaaca tcggcgaata atgcaggcca
    11641 ctgcgcggca ggatatcgga ggtcatcacc agatccagct cgccctgttg caacgcgggc
    11701 tgcgggtcaa acgtcacgcc cgatttaaaa tccatctcta cctgcggcca gttcttatgg
    11761 aaattttcta aagcgggtgt cagccactga atacagctat ggcactcaat ggcaatgcgc
    11821 agacgcgtct gctgcggttc attgcaggct tgcagtgcct ggctgatttg cggcagcacc
    11881 tggtttgcca gttgcaacag gatttctccc tgcggtgtaa agcgtagcgg ctggctctta
    11941 cgcacaaata gccggaagcc aaggcgttgt tccagatcgc taaactggtg agacagggcg
    12001 gattgcgtct gatgcaacgt tgccgcagcg gctgcgagcg agccgcagtt ccgcaacgct
    12061 tgtagcgttt tcaggtgttt tacttcgatc atgaaagtcc ttcacttcgg catgaataat
    12121 ttgcgcttga ggaatataca gtaaccgcca attatggatg tgtaaacatc tggacggcta
    12181 aaatcattcg tcttttaaat ttatggtgcg ttggctgcgt ttctccaccc cggtcactta
    12241 cttcagtaag ctcccggcga tgaataaact tgccgccttc cctaaattca aaatccatag
    12301 gatttacata taattagagg aagaaaaatg acaatattga atcacaccct cggtttccct
    12361 cgcgttggcc tgcgtcgcga gctgaaaaaa gcgcaagaaa gttattgggc ggggaactcc
    12421 acgcgtgaag aactgctgac agtagggcgt gaactgcgtg ctcgtcactg ggatcaacaa
    12481 aagcaagcgg gtatcgacct gctgccggtg ggcgattttg cctggtacga tcatgtactg
    12541 accaccagtt tgctgctggg taatgttccg ccacgtcatc agaacaaaga cggttcggta
    12601 gatatcgaca ccctgttccg tattggtcgt ggacgtgcgc cgactggcga acctgcggcg
    12661 gcagcggaaa tgaccaaatg gtttaacacc aactatcact acatggtgcc ggagttcgtt
    12721 aaaggccaac agttcaaact gacctggacg cagctgctgg aggaagtgga cgaggcgctg
    12781 gcgctgagcc acaacgttaa gcctgtgctg ctggggccgg ttacttacct gtggctgggg
    12841 aaagtgaaag gtgaacagtt tgaccgcctt agcctgctga acgacattct gccggtttat
    12901 cagcaagtgc tggcagaact ggcgaaacgc ggcatcgagt gggtacagat tgatgaaccc
    12961 gcgctggtac tggaactacc acaggcgtgg ctggacgcat acaaacccgc ttacgacgcg
    13021 ctccagggac aggtgaaact gctgctgacc acctattttg aaggcgtaac gccaaatctc
    13081 gacacgatta ctgcgctgcc tgttcagggg ctgcatgtgg accttgtaca tggtaaagat
    13141 aacgttgttg aactgcacaa gcgcctgcct tctgactggc tgctgtctgc gggtctgatc
    13201 aatggtcgta acgtctggcg cgccgatctt actgagaaat atgcgcaaat taaggacatt
    13261 gtcggcaaac gtgatttgtg ggtggcatct tcctgctcgt tgctgcacag ccccatcgac
    13321 ctgagcgtgg aaacgcgtct tgatgcagaa gtgaaaagct ggtttgcctt cgccctacaa
    13381 aaatgccatg aactggcact gctgcgcgat gcgctgaaca gtggtgacac ggcagctctg
    13441 gcagagtgga gcgccccaat tcaggcgcgt cgtcactcta ctcgtgtaca taatccggcg
    13501 gtagaaaagc gtctggcggc gatcaccgcc caggacagcc agcgtgcgaa tgtctatgaa
    13561 gtgcgtgctg aagcccagcg tgcgcgtttt aaactgccag cgtggccgac caccacgatt
    13621 ggttccttcc cgcaaaccac ggaaattcgt accctgcgtc tggatttcaa aaagggcaat
    13681 ctcgacgcca acaactaccg cacgggcatt gcggaacata tcaagcaggc cattgttgag
    13741 caggaacgtt tgggactgga tgtgctggta catggcgagg ccgagcgtaa tgacatggtg
    13801 gaatactttg gcgagcacct cgacggattt gtctttacgc aaaacggttg ggtacagagc
    13861 tacggttccc gctgcgtgaa gccaccgatt gtcattggtg acattagccg cccggcaccg
    13921 attaccgtgg agtgggcgaa gtatgcgcaa tcgctgaccg acaaaccggt gaaagggatg
    13981 ctgacggggc cggtgaccat actctgctgg tcgttcccgc gtgaagatgt cagccgtgaa
    14041 accatcgcca aacagattgc gctggcgctg cgtgatgaag tggccgatct ggaagccgct
    14101 ggaattggca tcatccagat tgacgaaccg gcgctgcgcg aaggtttacc gctgcgtcgt
    14161 agcgactggg atgcgtatct ccagtggggc gtagaggcct tccgtatcaa cgccgccgtg
    14221 gcgaaagatg acacacaaat ccacactcac atgtgttatt gcgagttcaa cgacatcatg
    14281 gattcgattg cggcgctgga cgcagacgtc atcaccatcg aaacctcgcg ttccgacatg
    14341 gagttgctgg agtcgtttga agagtttgat tatccaaatg aaatcggtcc tggcgtctat
    14401 gacattcact cgccaaacgt accgagcgtg gaatggattg aagccttgct gaagaaagcg
    14461 gcaaaacgca ttccggcaga gcgcctgtgg gtcaacccgg actgtggcct gaaaacgcgc
    14521 ggctggccag aaacccgcgc ggcactggcg aacatggtgc aggcggcgca gaatttgcgt
    14581 cgggggtaaa tccaaaccgg gtggtaatac cacccggtct tttctcatta cagcgacttc
    14641 ttcccaccat actgcttaaa ccattccagc atacgctgcc agccatcttt cgcagattcg
    14701 gcatgatagc tcgggcgata atcagcgttg aatgcatgcc cggcatccgg gtacacgata
    14761 atctctgctt tcgcattagc agcccgcagc gcctggcgca tcgtttcaac gctctcctgc
    14821 ggaatgctgt tatcctgacc accatataag ccgagaaccg gcgcgttaag atcggttgcg
    14881 atatcaacag gttgtttcgg tgaattcagc gacttgtcgc ccgtcagttt gccgtaccac
    14941 gccactgcgg cttttagctg tggattatgc gcggcataca gccaggtgat acgtccaccc
    15001 cagcagaatc cggtgatcat taaacgatga acatcgccgc cgttgcggga agcccaactg
    15061 gcgacatgat cgagatcggc cagcacctgc gagtcaggca cttttgctac cagaccgcta
    15121 agcaacgtgg ggatatcggc aaaatcattc ggatcgcctt cgcggaagta aagttcaggt
    15181 gcgatagcca gatacccctc cagcgccaga cggcgacaaa tgtcgcggat atgttcatgc
    15241 acgccaaaaa tttcctgcac tacaatgacc actggcagtg ggccatcgct ttgctttggt
    15301 ctggcatggt aagcaggcat gttatcccct tgtgaaggga tagaggtgaa gcccgccaca
    15361 atcgcgtcgt ccggggtctg aacgatggtc gaagcgagag gcgatgcagc aggtgcaaat
    15421 ccagattgtt gtgttgttgc catggtattc tccgtaccct tataaaaatg ttgcgcaatg
    15481 ttaactatag tcagcatgca acaaatcaca ttgcctgaat cggctcatct tttatgcagt
    15541 cctgcagaat gaagagtgat ttatgtgata tgaatcactt ttggtgggta aatttatgca
    15601 acgcatttgc gtcatggtga tgagtatcac gaaaaaatgt taaacccttc ggtaaagtgt
    15661 ctttttgctt cttctgacta aaccgattca cagaggagtt gtatatgtcc aagtctgatg
    15721 tttttcatct cggcctcact aaaaacgatt tacaaggggc tacgcttgcc atcgtccctg
    15781 gcgacccgga tcgtgtggaa aagatcgccg cgctgatgga taagccggtt aagctggcat
    15841 ctcaccgcga attcactacc tggcgtgcag agctggatgg taaacctgtt atcgtctgct
    15901 ctaccggtat cggcggcccg tctacctcta ttgctgttga agagctggca cagctgggca
    15961 ttcgcacctt cctgcgtatc ggtacaacgg gcgctattca gccgcatatt aatgtgggtg
    16021 atgtcctggt taccacggcg tctgtccgtc tggatggcgc gagcctgcac ttcgcaccgc
    16081 tggaattccc ggctgtcgct gatttcgaat gtacgactgc gctggttgaa gctgcgaaat
    16141 ctattggcgc gacaactcac gttggcgtga cagtttcttc tgataccttc tacccaggtc
    16201 aggaacgtta cgatacttac tctggtcgcg tagttcgtca ctttaaaggt tctatggaag
    16261 agtggcaggc gatgggcgta atgaactatg aaatggaatc tgcaactctg ctgaccatgt
    16321 gtgcaagtca gggcctgcgt gccggtatgg tagcgggtgt tatcgttaac cgcacccagc
    16381 aagagatccc gaatgctgag acgatgaaac aaaccgaaag ccatgcggtg aaaatcgtgg
    16441 tggaagcggc gcgtcgtctg ctgtaattct cttctcctgt ctgaaggccg acgcgttcgg
    16501 ccttttgtat ttttgcgtag cgcctcgcag gaaatgcctt tccaactgga cgtttgtaca
    16561 gcacaattct attttgtgcg ggtaagttgt tgcgtcagga ggcgttgtgg atttctcaat
    16621 catggtttac gcagttattg cgttggtggg tgtggcaatt ggctggctgt ttgccagtta
    16681 tcaacatgcg cagcaaaaag ccgagcaatt agctgaacgt gaagagatgg tcgcggagtt
    16741 aagcgcggca aaacaacaaa ttacccaaag cgagcactgg cgtgcagagt gtgaattgct
    16801 caataacgaa gtgcgcagcc tgcaaagtat taacacctct ctggaggccg atctgcgtga
    16861 agtaaccacg cggatggaag ccgcacagca acatgctgac gataaaattc gccagatgat
    16921 taacagcgag cagcgcctca gtgagcagtt tgaaaacctc gccaaccgta tttttgagca
    16981 cagcaatcgc cgggttgatg agcaaaaccg tcagagtctg aacagcctgt tgtcgccgct
    17041 acgtgaacaa ctggacggtt tccgccgtca ggttcaggac agcttcggta aagaagcaca
    17101 agaacgccat accctgaccc acgaaattcg caatctccag caactcaacg cgcaaatggc
    17161 ccaggaagcg atcaacctga cgcgcgcgct gaaaggcgac aataaaaccc agggcaactg
    17221 gggcgaggta gtattgacgc gggtgctgga ggcttccggt ctgcgtgaag ggtatgaata
    17281 tgaaacccag gtcagcatcg aaaatgacgc ccgctcgcgg atgcagccgg atgtcatcgt
    17341 gcggctgccg cagggaaaag atgtggtgat cgacgccaaa atgacgctgg tcgcctatga
    17401 acgctatttt aacgccgaag acgactacac ccgcgaaagc gcgctacagg aacatatcgc
    17461 gtcggtgcgt aaccatatcc gtttgctggg acgcaaagat tatcaacagc tgccggggct
    17521 gcgaactctg gattacgtgc tgatgtttat tcccgttgaa cccgcttttt tactggcgct
    17581 tgaccgccag ccggagctga tcaccgaagc gttgaaaaac aatatcatgc tggttagccc
    17641 gactacgctg ctggtggcgt tgcgtactat tgccaacctg tggcgttatg agcatcaaag
    17701 ccgtaacgcc cagcaaatcg ccgatcgtgc cagcaagctg tacgacaaga tgcgtttgtt
    17761 catcgatgac atgtccgcga ttggtcaaag tctcgacaaa gcgcaggata actaccgcca
    17821 ggcaatgaaa aaactctctt caggtcgcgg aaatgtgctg gcgcaggcag aagcgtttcg
    17881 cggtttagga gtagaaatta aacgcgagat taatccggat ttggctgaac aggcggtgag
    17941 ccaggatgaa gagtatcgac ttcggtcggt tccggagcag ccgaatgatg aagcttatca
    18001 acgcgatgat gaatataatc agcagtcgcg ctagcccatt aggggtagtt aagccgggta
    18061 gaaatctagg gcatcgacgc ccaatctgtt acacttctgg aacaattttt tgatgagcag
    18121 gcattgagat ggtggataag tcacaagaaa cgacgcactt tggttttcag accgtcgcga
    18181 aggaacaaaa agcggatatg gtcgcccacg ttttccattc cgtggcatca aaatacgatg
    18241 tcatgaatga tttgatgtca tttggtattc atcgtttgtg gaagcgattc acgattgatt
    18301 gcagcggcgt acgccgtggg cagaccgtgc tggatctggc tggtggcacc ggcgacctga
    18361 cagcgaaatt ctcccgcctg gtcggagaaa ctggcaaagt ggtccttgct gatatcaatg
    18421 aatccatgct caaaatggga cgcgagaagc tgcgtaatat cggtgtgatt ggcaacgttg
    18481 agtatgttca ggcgaacgct gaggcgctgc cgttcccgga taacaccttt gattgcatca
    18541 ccatttcgtt tggtctgcgt aacgtcaccg acaaagataa agcactgcgt tcaatgtatc
    18601 gcgtgctgaa acccggcggc cgcctgctgg tgcttgagtt ctcgaagcca attatcgagc
    18661 cgctgagcaa agcctatgat gcatactcct tccatgtgct gccgcgtatt ggctcactgg
    18721 tcgcgaacga cgccgacagc taccgttatc tggcagaatc catccgtatg catcccgatc
    18781 aggataccct gaaagccatg atgcaggatg ccggattcga aagtgtcgac tactacaatc
    18841 tgacggcagg ggttgtggcg ctgcatcgtg gttataagtt ctgacaggag accggaaatg
    18901 ccttttaaac ctttagtgac ggcaggaatt gaaagtctgc tcaacacctt cctgtatcgc
    18961 tcacccgcgc tgaaaacagc ccgctcgcgt ctgctgggta aagtattgcg cgtggaggta
    19021 aaaggctttt cgacgtcatt gattctggtg ttcagcgaac gccaggttga tgtactgggc
    19081 gaatgggcag gcgatgctga ctgcaccgtt atcgcctacg ccagtgtgtt gccgaaactt
    19141 cgcgatcgcc agcagcttac cgcactgatt cgcagtggtg agctggaagt gcagggcgat
    19201 attcaggtgg tgcaaaactt cgttgcgctg gcagatctgg cagagttcga ccctgcggaa
    19261 ctgctggccc cttataccgg tgatatcgcc gctgaaggaa tcagcaaagc catgcgcgga
    19321 ggcgcaaagt tcctgcatca cggcattaag cgccagcaac gttatgtggc ggaagccatt
    19381 actgaagagt ggcgtatggc acccggtccg cttgaagtgg cctggtttgc ggaagagacg
    19441 gctgccgtcg agcgtgctgt tgatgccctg accaaacggc tggaaaaact ggaggctaaa
    19501 tgacgccagg tgaagtacgg cgcctatatt tcatcattcg cactttttta agctacggac
    19561 ttgatgaact gatccccaaa atgcgtatca ccctgccgct acggctatgg cgatactcat
    19621 tattctggat gccaaatcgg cataaagaca aacttttagg tgagcgacta cgactggccc
    19681 tgcaagaact ggggccggtt tggatcaagt tcgggcaaat gttatcaacc cgccgcgatc
    19741 tttttccacc gcatattgcc gatcagctgg cgttattgca ggacaaagtt gctccgtttg
    19801 atggcaagct ggcgaagcag cagattgaag ctgcaatggg cggcttgccg gtagaagcgt
    19861 ggtttgacga ttttgaaatc aagccgctgg cttctgcttc tatcgcccag gttcataccg
    19921 cgcgattgaa atcgaatggt aaagaggtgg tgattaaagt catccgcccg gatattttgc
    19981 cggtcattaa agcggatctg aaacttatct accgtctggc tcgctgggtg ccgcgtttgc
    20041 tgccggatgg tcgccgtctg cgcccaaccg aagtggtgcg cgagtacgaa aagaccttga
    20101 ttgatgaact gaatttgctg cgggaatctg ccaacgccat tcagcttcgg cgcaattttg
    20161 aagacagccc gatgctctac atcccggaag tttaccctga ctattgtagt gaagggatga
    20221 tggtgatgga gcgcatttac ggcattccgg tgtctgatgt tgcggcgctg gagaaaaacg
    20281 gcactaacat gaaattgctg gcggaacgcg gcgtgcaggt gttcttcact caggtctttc
    20341 gcgacagctt tttccatgcc gatatgcacc ctggcaacat cttcgtaagc tatgaacacc
    20401 cggaaaaccc gaaatatatc ggcattgatt gcgggattgt tggctcgcta aacaaagaag
    20461 ataaacgcta tctggcagaa aactttatcg ccttctttaa tcgcgactat cgcaaagtgg
    20521 cagagctaca cgtcgattct ggctgggtgc caccagatac caacgttgaa gagttcgaat
    20581 ttgccattcg tacggtctgt gaacctatct ttgagaaacc gctggccgaa atttcgtttg
    20641 gacatgtact gttaaatctg tttaatacgg cgcgtcgctt caatatggaa gtgcagccgc
    20701 aactggtgtt actccagaaa accctgctct atgtcgaagg ggtaggacgc cagctttatc
    20761 cgcagctcga tttatggaaa acagcgaagc ctttcctgga gtcgtggatt aaagatcagg
    20821 tcggtattcc tgccctggtg agagcattta aagaaaaagc gccgttctgg gtcgaaaaaa
    20881 tgccagaact gcctgaactg gtttacgaca gtttgcgcca gggcaagtat ttacagcaca
    20941 gtgttgataa gattgcccgc gagcttcagt caaatcatgt acgtcaggga caatcgcgtt
    21001 attttctcgg aattggcgct acgttagtat taagtggcac attcttgttg gtcagccgac
    21061 ctgagtgggg gctgatgccc ggctggttaa tggcaggtgg tctgatcgcc tggtttgtcg
    21121 gttggcgcaa aacacgctga ttttttcatc gctcaaggcg ggccgtgtaa cgtataatgc
    21181 ggctttgttt aatcatcatc taccacagag gaacatgtat gggtggtatc agtatttggc
    21241 agttattgat tattgccgtc atcgttgtac tgctttttgg caccaaaaag ctcggctcca
    21301 tcggttccga tcttggtgcg tcgatcaaag gctttaaaaa agcaatgagc gatgatgaac
    21361 caaagcagga taaaaccagt caggatgctg attttactgc gaaaactatc gccgataagc
    21421 aggcggatac gaatcaggaa caggctaaaa cagaagacgc gaagcgccac gataaagagc
    21481 aggtgtaatc cgtgtttgat atcggtttta gcgaactgct attggtgttc atcatcggcc
    21541 tcgtcgttct ggggccgcaa cgactgcctg tggcggtaaa aacggtagcg ggctggattc
    21601 gcgcgttgcg ttcactggcg acaacggtgc agaacgaact gacccaggag ttaaaactcc
    21661 aggagtttca ggacagtctg aaaaaggttg aaaaggcgag cctcactaac ctgacgcccg
    21721 aactgaaagc gtcgatggat gaactacgcc aggccgcgga gtcgatgaag cgttcctacg
    21781 ttgcaaacga tcctgaaaag gcgagcgatg aagcgcacac catccataac ccggtggtga
    21841 aagataatga agctgcgcat gagggcgtaa cgcctgccgc tgcacaaacg caggccagtt
    21901 cgccggaaca gaagccagaa accacgccag agccggtggt aaaacctgct gcggacgctg
    21961 aaccgaaaac cgctgcacct tccccttcgt cgagtgataa accgtaaaca tgtctgtaga
    22021 agatactcaa ccgcttatca cgcatctgat tgagctgcgt aagcgtctgc tgaactgcat
    22081 tatcgcggtg atcgtgatat tcctgtgtct ggtctatttc gccaatgaca tctatcactt
    22141 ggtatccgcg ccattgatca agcagttgcc gcaaggttca acgatgatcg ccaccgacgt
    22201 ggcctcgccg ttctttacgc cgatcaagct gacctttatg gtgtcgctga ttctgtcagc
    22261 gccggtgatt ctctatcagg tgtgggcatt tatcgcccca gcgctgtata agcatgaacg
    22321 tcgcctggtg gtgccgctgc tggtttccag ctctctgctg ttttatatcg gcatggcatt
    22381 cgcctacttt gtggtctttc cgctggcatt tggcttcctt gccaataccg cgccggaagg
    22441 ggtgcaggta tccaccgaca tcgccagcta tttaagcttc gttatggcgc tgtttatggc
    22501 gtttggtgtc tcctttgaag tgccggtggc aattgtgctg ctgtgctgga tggggattac
    22561 ctcgtcagaa gacttacgca aaaaacgccc gtatgtgctg gttggtgcat tcgttgtcgg
    22621 gatgttgctg acgccgccgg atgtcttctc gcaaacgcta ttggcgatcc cgatgtactg
    22681 tctgtttgaa atcggtgtct tcttctcacg cttttacgtt ggtaaagggc gaaatcggga
    22741 agaggaaaac gacgctgaag cagaaagcga aaaaactgaa gaataaattc aaccgcccgt
    22801 cagggcggtt gtcatatgga gtacaggatg tttgatatcg gcgttaattt gaccagttcg
    22861 caatttgcga aagaccgtga tgatgttgta gcgcgcgctt ttgacgcggg agttaatggg
    22921 ctactcatca ccggcactaa cctgcgtgaa agccagcagg cgcaaaagct ggcgcgtcag
    22981 tattcgtcct gttggtcaac ggcgggcgta catcctcacg acagcagcca gtggcaagct
    23041 gcgactgaag aagcgattat tgagctggcc gcgcagccag aagtggtggc gattggtgaa
    23101 tgtggtctcg actttaaccg caacttttcg acgccggaag agcaggaacg cgcttttgtt
    23161 gctcagctac gcattgccgc agaattaaac atgccggtat ttatgcactg tcgcgatgcc
    23221 cacgagcggt ttatgacatt gctggagccg tggctggata aactgcctgg tgcggttctt
    23281 cattgcttta ccggcacacg cgaagagatg caggcgtgcg tggcgcgtgg aatttatatc
    23341 ggcattaccg gttgggtttg cgatgaacga cgcgggctgg agctgcggga attgttgccg
    23401 ttgattccgg cggagaaatt gctgatcgaa acagatgcgc cgtatctgct cccgcgcgat
    23461 ctcacgccaa agccatcatc ccggcgcaac gagccagccc atctgcccca tattttgcaa
    23521 cgtattgcgc actggcgtgg agaagatgcc gcatggctgg ctgccaccac ggatgccaat
    23581 gtcaaaacac tgtttgggat tgcgttttag agtttgcgga actcggtgtt cttcacactg
    23641 tgcttaatct ctttattaat aagattaagc aatagcatgg agcgagcctc accatcgggt
    23701 tcggtgaaaa tggcctgaaa gccttcgaac gcgccttcgg taataatcac cttatcaccc
    23761 ggataagggg gggccggatc gacaatgtct ttcggtttat ataccgatag ctgatgaatt
    23821 accgccgatg ggactatcgc tggcgacgcg ccaaagcgca caaagtggct gacgccacgc
    23881 gtagcgctga tggtcgtggt atgaatcact tctggatcaa attccacaaa caggtagtta
    23941 gggaataacg gttcactgac tgcggtacgt ttaccccgta cgattttttc cagggtgatc
    24001 atcggagcga ggcaattaac cgcctgtctc tcaaggtgtt cctgggcacg ttgaagttgc
    24061 ccgcgcttgc agtacagtaa ataccaggat tgcataatga ctcttatccg tttaatcggg
    24121 gcgcaaggat agcaaaagct ttacgctaag ttaattatat tccccggttt gcgttatacc
    24181 gtcagagttc acgctaattt aacaaattta cagcattgca aagatgaacg ccgtataatg
    24241 ggcgcagatt aagaggctac aatggacgcc atgaaatata acgatttacg cgacttcctg
    24301 acgctgcttg aacagcaggg tgagctaaaa cgtatcacgc tcccggtgga tccgcatctg
    24361 gaaatcactg aaattgctga ccgcactctg cgtgctggtg ggcctgcgct gttgttcgaa
    24421 aaccctaaag gctactcaat gccggtgctg tgcaacctgt tcggtacgcc aaagcgcgtg
    24481 gcgatgggca tggggcagga agatgtttcg gcgctgcgtg aagttggtaa attattggcg
    24541 tttctgaaag agccggagcc gccaaaaggt ttccgcgacc tgtttgataa actgccgcag
    24601 tttaagcagg tgttaaacat gccgacaaag cgactgcgtg gtgcgccctg ccaacaaaaa
    24661 atcgtctctg gcgatgacgt cgatctcaat cgcattccca ttatgacctg ctggccggaa
    24721 gatgccgcgc cgctgattac ctgggggctg accgtaacgc gcggcccgca taaagagcgg
    24781 cagaatctgg gcatttatcg ccagcagctg attggtaaaa acaaactgat tatgcgctgg
    24841 ctgtcgcatc gcggcggcgc gctggattat caggagtggt gtgcggcgca tccgggcgaa
    24901 catttcccgg tttctgtggc gctgggtgcc gatcctgcca cgattctcgg tgcagtcacc
    24961 cccgttccgg atacgctttc agagtatgcg tttgccggat tgctacgcgg caccaaaacc
    25021 gaagtagtaa agtgtatttc caatgacctc gaagtgccag ccagtgccga aatcgtcctg
    25081 gaagggtata tcgatcctgg tgagatggcg ccggaagggc cgtatggtga ccacacaggt
    25141 tactataatg aagtcgatag tttcccggtg tttaccgtga cgcatattac ccagcgtgaa
    25201 gatgcgattt accattccac ctataccggg cgtccgccag atgagcccgc ggtactgggc
    25261 gtggcgttga acgaagtgtt tgtaccgatt ctgcaaaaac agttcccgga aattgtcgat
    25321 ttttacctgc cgccggaagg ctgttcttat cgtctggcgg tagtgacgat caaaaaacag
    25381 tacgccggac acgcgaagcg cgtcatgatg ggcgtctggt cgttcttacg ccagtttatg
    25441 tacactaaat ttgtgatcgt ttgcgatgat gacgtcaacg cacgcgactg gaacgatgtg
    25501 atttgggcga ttaccacccg tatggacccg gcgcgggata ctgttctggt agaaaatacg
    25561 cctattgatt atctggattt tgcctcgcct gtctctgggc tgggttcaaa aatggggctg
    25621 gatgccacga ataaatggcc gggggaaacc cagcgtgaat ggggacgtcc catcaaaaaa
    25681 gatccagatg ttgtcgcgca tattgacgcc atctgggatg aactggctat ttttaacaac
    25741 ggtaaaagcg cctgatgcgc gtttgttttg ccctatttat cgatccgaca gagaaagcgc
    25801 atgacaacct taagctgtaa agtgacctcg gtagcagcta tcacggatac cgtatatcgt
    25861 gtccgcatcg tgccagacgc ggccttttct tttcgtgctg gtcagtattt gatggtagtg
    25921 atggatgagc gcgacaaacg tccgttctca atggcctcga cgccggatga aaaagggttt
    25981 atcgagctgc atattggcgc ttctgaaatc aacctttacg cgaaagcagt catggaccgc
    26041 atcctcaaag atcatcaaat cgtggtcgac cttccccacg gagaagcgtg gctgcgcgat
    26101 gatgaagagc gtccgatgat tttgattgcg ggcggcaccg ggttctctta tgcccgctcg
    26161 attttgctga cagcgttggc gcgtaaccca aaccgtgata tcaccattta ctggggcggg
    26221 cgtgaagagc agcatctgta tgatctctgc gagcttgagg cgctttcgtt aaagcacctt
    26281 ggtctgcaag tggtgccggt ggttgaacaa ccggaagcag gctggcgtgg gcgtactggt
    26341 accgtgttaa cggcggtatt gcaggatcat ggtacgctgg cagagcatga tatctatatt
    26401 gccggacgtt ttgagatggc gaaaattgcc cgtgacctgt tttgcagtga gcgtaatgcg
    26461 cgggaagatc gcctgtttgg cgatgcgttt gcatttatct gagatataaa aaaacccgcc
    26521 cctgacaggc gggaagaacg gcaactaaaa ctttattcag tggcatttag atctatgtcg
    26581 catccggcaa ttggtgcacg atgcctgatg cgacgctggc gcgtcttatc aggcctacat
    26641 tggtgccgga tcggtagacc ggataaggcg ttcacgccgc atccggcaag tggttaaacc
    26701 cgctcaaaca ccgtcgcaat accctgaccc agaccgatac acatcgtcgc cagaccaaac
    26761 tgaacgtctt tgcgttccat cagattcagc agcgtggtgc tgatacgcgc accggaacaa
    26821 cccagcggat gacccagcgc gatcgcacca ccattgaggt tgatcttctc gtcaatctgc
    26881 tccattagcc cgagatcttt aatgcacggc aggatctgcg cggcaaaggc ttcgttcatc
    26941 tcaaacacgc cgatatcgct ggcagaaagc cccgcttttt tcagcgccag cttcgaggcc
    27001 ggaaccgggc cgtaacccat aatcgatggg tcacaaccaa cgaccgccat cgaacgcaca
    27061 cgagcgcgcg gcttaagacc taattcatgg gcgcggcttt cactcatcac cagcatggca
    27121 gctgcgccat cggaaagtgc agaagatgtg cccgccgtta ccgtaccgtt tactggatca
    27181 aacgccggac gcagcgtggc gagggcttcc acggtggttt ccgggcgaat cacttcgtcg
    27241 taattaaact gcttcaggac gccgtcggca tcgtgaccac cagttgggat gatttcattt
    27301 ttaaatgcgg ccgactgcgt ggcggcccag gcgcgggcgt gtgaccgcgc ggcaaaggca
    27361 tcctgcattt cacggctgat accgtgcata cgcgccagca tttctgccgt taagcccatc
    27421 atgcccgccg ctttggcgac attgcggctc aggccggggt gaaaatcgac gccgtgactc
    27481 atcggcacat ggcctatatg ctccacgccg ccaaccagac atgcctgcgc atcgccagtc
    27541 atgatcattc gtgctgcgtc atgcagtgcc tgcatggatg aaccacacaa gcgattgacg
    27601 gtaaccgccg ggacagagtg cggcacttct gccagcagcg ccgcgttacg ggcgatgtta
    27661 aaaccctgct ccagcgtctg ttgcacacaa ccccagtaaa tatcgtcgag ggccgccgct
    27721 tccagcgccg ggttacgcgc cagcaggcta cgcattaaat gagcggagag atcttccgca
    27781 cgcacgttac gaaaagcacc gcccttcgaa cggcccatcg gggtgcgaat tgcatcgaca
    27841 atgacaacct gttccattgt gactccttaa gccgttttca ggtcgccaac cggacgggct
    27901 ggctcaaccg gaggatagta cggttcgtta tgacgcgctt tattacgcag accttccggc
    27961 acttcataca gcgggccgag gtgctgatat tgctgtgcca tatcgaggta ttttgcgcta
    28021 ccgagggtgt ccagccagcg gaacgcgccg ccgtggaacg gagggaagcc caggccgtag
    28081 accagcgcca tatccgcttc cgccggagtg gcgataatgc cctcttccag acagcgcacc
    28141 acttcgttga ccatcgggat catcatgcgg gcgataatct cttcttcgct gaaatcgcgc
    28201 ttcggttggc tcacttctgc cagcaggtct tcaacggcgg cgtcttcttc tttcttcggc
    28261 ttacctttgc tgtcttcttt ataacgccag aaaccgaggc cgttcttctg accaaagcgg
    28321 ttggcatcaa acagcgcgtc gatggcatcg cggtaatctt tctgcatccg ctgcgggaag
    28381 cctgctgcca tgacagcctg agcgtgatgc gcggtatcaa tgcccacaac gtccagcaga
    28441 tatgccgggc ccatcggcca gccaaactgt ttttccatca ctttgtcgat cttgcggaaa
    28501 tccgcgccgt cgcgcagcag ctggctgaaa ccggcgaaat acgggaacag cacgcggtta
    28561 acaaagaagc cggggcagtc gttaaccaca atcggcgtct tgcccatctt gctcgcccag
    28621 gcgacaactt tcgcgatggt ttcgtcggag cttttctcgc cgcgaataat ttctaccaac
    28681 ggcattcggt ggaccgggtt aaagaagtgc atcccgcaga agttttccgg gcgttccagc
    28741 gcgttggcca gttcgctgat aggaatggtt gaagtgttag acgccagcac ggtatcctgg
    28801 cgtacttttt gttcggtttc tgccagtacg gcttttttca ctttcgggtt ttcaacaacc
    28861 gcttctacca caatatccac gcggtcaaat ccggcgtagt cgagcgttgg gtggatcgtg
    28921 gagatcacgc cagccagttt cagaccatcg attttgccgc gctcaagctg cttgttcagc
    28981 agtttcgcgg cttcggtcat gccgagggtt aacgacttgt cgttgatatc tttcatgaca
    29041 accggcacgc ctttccacgc ggactggtaa gcgataccgc cacccatgat gcctgcgccc
    29101 agcacagcag cctgtttcgg ggtttcaacg tctttggtga gtttcttcgc tttgcctttt
    29161 acatattgat cgttaaggaa aatgccgacc agtgcgcgag cttcgttggt atgcgccagc
    29221 gggacaaaac ttttgttttc cagatttaag gcttcttcac gaccaaaacg ggccgcagct
    29281 tcaatggttt ttactgcggt gatgggggcc ggataatgtt tccccgccgt ttgtgcgacc
    29341 attcctttag cgatggtgaa gctcatggtg gcttcaatct tgctcagttt tagcggttcc
    29401 tgcttcggct gacgtttcgc tttccagtcg aggtcgccgt taatggcctg gcgtaaaatc
    29461 gccatcgccc cttcaatcag tttttctgct ttgactacgc catccaccag accgattttc
    29521 agcgcctgat ccgcgccgac atctttaccg gcggcaatga tttccagtgc actgtcagcg
    29581 cccagcatac gtggcatacg tacagaaccg ccaaagcccg gcatgatgcc cagtttggtt
    29641 tccggcagac cgatgcgcag atccggcgtc gccagacgat aatcggtcgc cagcacgcat
    29701 tcgcagccac cacccagcgc atagccgttg acggcagcaa tggtcggcac cggcagatct
    29761 tccaggcgat taaacacgct attggcaaag tgcagccact gacttaactg ttcttcagga
    29821 acgaggaaca gggacaaaaa ttcggtgata tcagcaccga cgataaaggc tgctttgttc
    29881 gaacgcagca gcagcccttt tagatctgat tgctgttcca gcacgccgat ggcctcgccg
    29941 aggctggcga cggtcgcagt gtcgagttta ttaactgaac ctggggcatc aaataccagt
    30001 tcggcaatgc catcttccag ccagtcaagg tacagggtgt cgcctttgta aagcatgtca
    30061 gtctcctgaa tccgcaaggt gatctggtcg taccagatgc atcggagtgt gtgttttgtg
    30121 ttaaaaatat gcaaataaaa gattaaagaa atgccgctct gatcacgctc ggcagaaatc
    30181 acgctctgga tgaacgatgt gctaagatgc ggagacttaa ggtcaaaaaa acagaagggt
    30241 aaaaaatgga atcactggcc tcgctctata aaaatcatat agctacctta caagaacgga
    30301 ctcgcgatgc gctggcgcgc ttcaagctgg atgcattact tattcactcc ggcgagctgt
    30361 tcaacgtttt tctcgacgat catccctatc cgtttaaagt gaacccgcaa ttcaaagcgt
    30421 gggtgccggt aactcaggtg ccaaactgct ggttgctggt ggatggcgtg aacaagccga
    30481 aactgtggtt ctatctgccg gttgattact ggcacaacgt cgaaccgctg ccgacctcct
    30541 tctggactga agatgtggaa gtgatcgcgc tgccgaaagc cgatggcatt ggtagcctgc
    30601 tgcctgctgc gcgcggcaat atcggttata tcggtccggt gccggaacgt gcgctgcaac
    30661 tgggtattga ggccagcaac atcaacccga aaggggtgat cgactacctg cattattacc
    30721 gctccttcaa aaccgagtac gaactggcct gtatgcgtga agcgcagaaa atggcggtca
    30781 atggtcatcg tgcggcagaa gaagcgttcc gttctggcat gagcgagttc gatatcaata
    30841 ttgcctatct gaccgcgacc ggtcatcgtg ataccgacgt accttacagc aacattgtgg
    30901 cgcttaacga acacgctgcg gtgttgcatt acaccaaact ggaccaccag gcaccggaag
    30961 agatgcgcag cttcctgctg gatgccgggg ccgaatataa cggctatgcg gctgacctga
    31021 cccgtacctg gtcggcaaaa agcgacaacg actacgcaca gctggtgaaa gacgtaaatg
    31081 atgaacaact ggcgctgatc gccaccatga aagcaggcgt cagctatgtg gattaccaca
    31141 tccagttcca tcagcgcatc gccaaactgc tgcgtaaaca tcaaatcatc accgatatga
    31201 gtgaagaagc gatggtcgaa aacgatctca ccggaccgtt tatgccgcac ggtattggcc
    31261 atccgctggg cctgcaggtg catgacgtcg ccggttttat gcaggatgat agcggtacac
    31321 acctcgcggc accggcaaaa tatccgtacc tgcgctacac ccgtattctc cagccgggca
    31381 tggtgttaac catcgaaccg ggtatctact tcattgaatc gcttctggca ccgtggcgtg
    31441 aagggcagtt cagcaagcac ttcaactggc agaaaattga agcactgaaa ccgttcggcg
    31501 gcattcgtat cgaagacaac gtggtgatcc acgaaaacaa cgtggaaaac atgacccggg
    31561 atctgaaact ggcgtgatgg aaagctggtt aattcctgcg gcaccggtca cggtcgttga
    31621 agagatcaaa aagagccgct tcataacgat gttggcgcat accgatggcg ttgaggcggc
    31681 gaaagcgttt gttgaatcgg tgcgggcaga acaccccgat gcccgccacc attgcgtggc
    31741 gtgggtggcg ggtgcgccgg atgattctca acagctgggt ttctctgacg acggggagcc
    31801 ggcgggaacg gcaggtaaac cgatgctcgc ccagctaatg ggcagcggcg tcggggaaat
    31861 taccgctgtg gtagtgcgct actacggcgg catattgctt ggcaccggtg ggttagtgaa
    31921 agcgtatggc ggcggcgtga atcaggcgct gcgccagcta acgacccaac gcaagacgcc
    31981 attaaccgaa tatactttgc aatgtgaata tagtcagtta accggcattg aagcgttgct
    32041 ggggcagtgt gacggcaaaa ttatcaacag tgattatcag gcattcgttc tgctgcgggt
    32101 ggcgcttccg gcggcgaaag tggctgaatt ttccgcaaag ctggcggatt ttagccgtgg
    32161 ttcattgcaa ttgttagcga ttgaagaata atccccactt cgttttgcag aactaaggaa
    32221 gcggcagaga tgcattttcg cgccattacc cgaatcgttg gactactggt catcttattt
    32281 tcagggacca tgattatccc tgggctggta gcactcatct accgggatgg agcgggccgc
    32341 gcttttaccc agaccttttt tgtcgccctc gccattggct ctatgctgtg gtggccgaac
    32401 cgcaaagaga agggcgaact gaaatcccgt gaggggtttc tgatagtggt gctgttttgg
    32461 accgtgctgg gtagtgtcgg tgcgctccct tttatcttct cggaaagccc gaacctcacg
    32521 attaccgatg cgttttttga atctttctct ggcctgacca ccaccggggc cactacgctg
    32581 gtggggctgg attcgctccc tcatgccatc ctcttttatc gccagatgct gcaatggttt
    32641 ggcgggatgg ggatcatcgt gttagcggtt gcgatactgc ctatcctcgg cgtgggtggg
    32701 atgcagctct atcgcgcaga aatgcccggc ccgctgaaag ataacaaaat gcgcccgcga
    32761 attgcggaaa cggcgaaaac tctgtggttg atttatgtct tgctgaccgt cgcctgtgcg
    32821 ctggcgttgt ggtttgccgg aatggatgcc tttgatgcca tcggccatag ctttgcgact
    32881 atcgctattg gcggcttctc gacacatgat gccagtatcg gttatttcga cagtccgact
    32941 attaacacta tcattgctat cttcctgctg atctccggct gtaactacgg tctgcacttt
    33001 tcactgttaa gtgggcgtag tctgaaggtt tattggcgcg atccggaatt tcgcatgttt
    33061 atcggcgtac agtttacgct ggtggttatt tgtacactcg tactgtggtt tcataatgtc
    33121 tacagttcgg cgctgatgac aattaaccag gcgtttttcc aggtggtatc gatggcgaca
    33181 accgccgggt ttacgactga cagcattgcc cgctggccgc tctttttgcc ggtactgctt
    33241 ttatgttcag cttttatcgg cggttgcgcc gggtcaacgg gcggtggcct gaaagtgatc
    33301 cgcatcctgc tgctgtttaa gcaggggaac cgtgagctga aacgactggt gcatccgaac
    33361 gccgtctata gcattaagct ggggaatcgc gcactgccgg aacgtatcct cgaagccgtt
    33421 tggggatttt tctccgccta tgcattggtg tttattgtca gtatgctggc gattatcgcc
    33481 acgggcgtgg atgatttttc tgcctttgcc tcagttgttg cgacattgaa taacctgggg
    33541 ccagggcttg gcgtggttgc tgataacttt accagtatga acccggtggc taaatggatc
    33601 ctgattgcca acatgctgtt tggtcgtctc gaggtcttta cattactggt gctctttacc
    33661 ccgactttct ggcgtgaatg atggagtaat acgtgaaaac attaattctt ttctcaacaa
    33721 gggacggaca aacgcgcgag attgcctcct acctggcttc ggaactgaaa gaactgggga
    33781 tccagacgga tgtcgccaat gtgcaccgca ttgaagaacc acaatgggaa aactatgacc
    33841 gtgtggtcat tggtgcttct attcgctatg gtcactacca ctctgcgttc caggaatttg
    33901 tcaaaaaaca tgcgacgcga ctgaattcga tgccgagcgc cttttattcc gtaaacctgg
    33961 tggcgcgcaa accggagaag cgtactccac agaccaacag ctacgcgcgg aagtttctga
    34021 tgaactcgca atggcgtccc gatcgctgcg cggtcattgc cggggcgctg cgttacccac
    34081 gttatcgctg gtacgaccgt tttatgctca agctgattat gaagatgtca ggcggtgaaa
    34141 cggatacgcg caaagaagtt gtctataccg attgggagca ggtggcgaat ttcgcccgag
    34201 aaatcgccca tttaaccgac aaaccgacgc tgaaataagc ataaagaata aaaaatgcgc
    34261 ggtcagaaaa ttattttaaa tttcctcttg tcaggccgga ataactccct ataatgcgcc
    34321 accactgaca cggaacaacg gcaaacacgc cgccgggtca gcggggttct cctgagaatt
    34381 ccgacagaga aagcaaaaat aaatgcttga ctctgtagcg ggaaagcgta ttatgaacac
    34441 cctgcgc
//
LOCUS       DAEEUE010000036        34086 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.036, whole genome shotgun sequence.
ACCESSION   DAEEUE010000036 DAEEUE010000000
VERSION     DAEEUE010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34086
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.036"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..578
                     /locus_tag="KLK84_001864"
     CDS             <1..578
                     /locus_tag="KLK84_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE2009211.1"
                     /translation="ERNAFYEGVEFPDDCDVLISYAPPKDESLVASFITQYAVTAYAS
                     QRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRDYRVKGNYVCDNT
                     QSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVAIFRKREYLPWRV
                     QYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            complement(575..1711)
                     /locus_tag="KLK84_001865"
     CDS             complement(575..1711)
                     /locus_tag="KLK84_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBE2009212.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTISTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1717..2040)
                     /locus_tag="KLK84_001866"
     CDS             complement(1717..2040)
                     /locus_tag="KLK84_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HBE2009213.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQIHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            2223..2366
                     /locus_tag="KLK84_001867"
     CDS             2223..2366
                     /locus_tag="KLK84_001867"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009214.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(2585..4123)
                     /gene="aldB"
                     /locus_tag="KLK84_001868"
     CDS             complement(2585..4123)
                     /gene="aldB"
                     /locus_tag="KLK84_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HBE2009215.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(4288..5439)
                     /gene="yiaY"
                     /locus_tag="KLK84_001869"
     CDS             complement(4288..5439)
                     /gene="yiaY"
                     /locus_tag="KLK84_001869"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HBE2009216.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(5629..7473)
                     /gene="selB"
                     /locus_tag="KLK84_001870"
     CDS             complement(5629..7473)
                     /gene="selB"
                     /locus_tag="KLK84_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBE2009217.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(7470..8861)
                     /gene="selA"
                     /locus_tag="KLK84_001871"
     CDS             complement(7470..8861)
                     /gene="selA"
                     /locus_tag="KLK84_001871"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBE2009218.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(8959..9567)
                     /locus_tag="KLK84_001872"
     CDS             complement(8959..9567)
                     /locus_tag="KLK84_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HBE2009219.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(9638..10723)
                     /locus_tag="KLK84_001873"
     CDS             complement(9638..10723)
                     /locus_tag="KLK84_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBE2009220.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNSFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(10726..11088)
                     /locus_tag="KLK84_001874"
     CDS             complement(10726..11088)
                     /locus_tag="KLK84_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HBE2009221.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            11625..13538
                     /gene="mtlA"
                     /locus_tag="KLK84_001875"
     CDS             11625..13538
                     /gene="mtlA"
                     /locus_tag="KLK84_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBE2009222.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            13633..14781
                     /gene="mtlD"
                     /locus_tag="KLK84_001876"
     CDS             13633..14781
                     /gene="mtlD"
                     /locus_tag="KLK84_001876"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBE2009223.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            14781..15368
                     /gene="mtlR"
                     /locus_tag="KLK84_001877"
     CDS             14781..15368
                     /gene="mtlR"
                     /locus_tag="KLK84_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HBE2009224.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(15380..15589)
                     /locus_tag="KLK84_001878"
     CDS             complement(15380..15589)
                     /locus_tag="KLK84_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009225.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            15873..16235
                     /locus_tag="KLK84_001879"
     CDS             15873..16235
                     /locus_tag="KLK84_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HBE2009226.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
     gene            16779..17462
                     /locus_tag="KLK84_001880"
     CDS             16779..17462
                     /locus_tag="KLK84_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HBE2009227.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            17506..21871
                     /locus_tag="KLK84_001881"
                     /pseudo
     CDS             17506..21871
                     /locus_tag="KLK84_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033191.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            22239..23894
                     /gene="lldP"
                     /locus_tag="KLK84_001882"
     CDS             22239..23894
                     /gene="lldP"
                     /locus_tag="KLK84_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HBE2009228.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            23894..24670
                     /gene="lldR"
                     /locus_tag="KLK84_001883"
     CDS             23894..24670
                     /gene="lldR"
                     /locus_tag="KLK84_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HBE2009229.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            24667..25857
                     /gene="lldD"
                     /locus_tag="KLK84_001884"
     CDS             24667..25857
                     /gene="lldD"
                     /locus_tag="KLK84_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HBE2009230.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTILLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            26053..26526
                     /gene="trmL"
                     /locus_tag="KLK84_001885"
     CDS             26053..26526
                     /gene="trmL"
                     /locus_tag="KLK84_001885"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBE2009231.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(26579..27400)
                     /gene="cysE"
                     /locus_tag="KLK84_001886"
     CDS             complement(26579..27400)
                     /gene="cysE"
                     /locus_tag="KLK84_001886"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBE2009232.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPVRIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(27480..28499)
                     /gene="gpsA"
                     /locus_tag="KLK84_001887"
     CDS             complement(27480..28499)
                     /gene="gpsA"
                     /locus_tag="KLK84_001887"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBE2009233.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(28499..28966)
                     /gene="secB"
                     /locus_tag="KLK84_001888"
     CDS             complement(28499..28966)
                     /gene="secB"
                     /locus_tag="KLK84_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HBE2009234.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(29029..29280)
                     /gene="grxC"
                     /locus_tag="KLK84_001889"
     CDS             complement(29029..29280)
                     /gene="grxC"
                     /locus_tag="KLK84_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HBE2009235.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(29422..29853)
                     /locus_tag="KLK84_001890"
     CDS             complement(29422..29853)
                     /locus_tag="KLK84_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBE2009236.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            30098..31642
                     /gene="gpmM"
                     /locus_tag="KLK84_001891"
                     /gene_synonym="pgmI"
     CDS             30098..31642
                     /gene="gpmM"
                     /locus_tag="KLK84_001891"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBE2009237.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            31652..32935
                     /gene="envC"
                     /locus_tag="KLK84_001892"
     CDS             31652..32935
                     /gene="envC"
                     /locus_tag="KLK84_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HBE2009238.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            32939..33898
                     /locus_tag="KLK84_001893"
     CDS             32939..33898
                     /locus_tag="KLK84_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HBE2009239.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(33885..34086)
                     /locus_tag="KLK84_001894"
                     /pseudo
     CDS             complement(33885..34086)
                     /locus_tag="KLK84_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         8470 a         8929 c         8690 g         7997 t
ORIGIN      
        1 ttgagcgtaa tgccttttat gaaggcgtcg aatttcctga tgattgtgat gttttaatca
       61 gctacgctcc accaaaagat gaatcgctgg ttgccagttt tattacacaa tatgccgtaa
      121 ccgcttatgc cagccagcgc tatcttgaga aacatcctat tagccgtcct gacgaactcg
      181 aacatcactc ctgtattttg atcgactcga tgatgatcga cgacgcgaat atctggcgct
      241 tcaacgtcgc cgggagtaaa gaggtgcggg attatcgcgt gaagggaaat tacgtttgcg
      301 ataacacgca atcggcgctg gagctggcac gaaatcacct ggggattgtg tttgcgccag
      361 acaaaagtgt gcagagcgat ctgcaagacg gcacgctggt gccctgcttt cagcacccgt
      421 atgaatggtg gctggatctg gtggctatct tccgtaaacg ggaatacctg ccctggcggg
      481 tccagtatgt gctggatgaa atgctgcgtg aaattcgcca ccagcttgct cagtcgcagc
      541 aactgcggcc tgaacaagcg gcggaaagcg aagattaatg atcgagataa aggtaatgca
      601 cccagctggt catgcgtaat agcactttgc gcatcaccga gacatggctg aaatgatcag
      661 agtagaccgc cacctgggcg taaataccgt ctggtaaagc gctcaaatca gtgtgctcat
      721 ccagttcaat ggtcgcgata acgccatctg aacccggcgc tgtgtttaac gtctgtaagg
      781 tgccggtcga ctgataagct ccgccgggaa cagcaggact aatggctgcc agcttgccgc
      841 tgaatacctt acctggcaga gcattaaaca ccacttccgc atcgtcgcca ggggccagtc
      901 gcagcaagga gttctgacgg aactgcgcca caatttgtcg tttctgatcg ggtataaaca
      961 ccatcaccgg acgtagcggc agcgacgcgg cataggtacc cggacgaatc agcacctggg
     1021 tcacataacc atcgctcggc gcacgcacta tcgtctgctc aaggttatat tttgcttccg
     1081 cgagctgcgc tttcaggctg gcgatttgag aatgttcacc caacaccagg ctatccagct
     1141 ggctctggat ctgtttttgt tccgccgccg atgactttac ggaggcttcc tgcgccagat
     1201 aattttgccg cgccacatca atatcgcgtt ctgaaaacgg gtttactttc gcctggctgc
     1261 cgcgtgcgta acgctgatac tctttagcga atttatcccg cgtggccttt gcctgctgag
     1321 tattcgccgc catctcatct aactctgcgc ccaacgcccg ctgtttatgt tctgcggtaa
     1381 cgatatccgc catcagccga tccacccgcg cctgataacg cgtcgggtcc agtcgaaata
     1441 gcacctcacc ttttttaatc agcgtatttt tcttatccgt cacttcgatc accacgcctg
     1501 tcacctgtgg gacaacagga atagaaataa ccgctttttg cgccttgaag gtatacggat
     1561 ggttatagtt cattaacaga attaaaccac tgacaataaa tatgccaccc agggccgctg
     1621 tggaaatggt ccatttattt acaggaattt tgaagatctt aaacattgcc catgcaaaag
     1681 ccacataggt caaaataatc agtaaatcca taagtattac tcggcagaag aggatttgat
     1741 gtcggcgagc tgtttctcca gcccggcaat acgctgttgt agttgcacaa cggaggagtc
     1801 atggatttgc attccccatc cccgctccgg gcggtaaagc gtggcccaaa tccacaaaaa
     1861 tggccagata acatgcagcg taaaaagact cacccaacca gcaacatgaa tggcgtcggc
     1921 atgaggatga ttacgttttt tagcaatcag gtagggaata tcatgtaaaa taataatccc
     1981 atagaatatc accagaaata caaaaataag cactcccagt gcaaaatagt ccaggaacat
     2041 atttccctca aagaatataa aaaagaacaa ttaacgcata ttatgaaatg ccatgatgca
     2101 gtgcaatttc cttatttata taacgacata ttgtttttta tatactttct gaaatgtgct
     2161 gtctggtttt tcaacaggac gaggtttatc atattgaaat agtggaacta ttggccaaac
     2221 taatgaataa ctccagtaaa acatccacag tacagattaa gcgtattaaa ccttcaatta
     2281 tctaccgttt attgctgatt ggcctcggat caccaatggt gatttacggc ctggttcgcc
     2341 cgctcaccat cgaaacgcga gattaaactc ctgacaaaac acgccccaga aacgcacttc
     2401 tgttggcgta gtctgggtta ttgcgcacgt aggtttaaaa cgtaaattgt aacaacgtgc
     2461 gctttgttta tgccggatgc ggcgtgtacg ccttatccgg cctacggtca ggttcccgta
     2521 ggcatgataa gacgcgtaag cgtcgcatca ggcaatgaat acccaatgcg accagcttct
     2581 tatatcagaa cagccccaac ggtttatccg agtagctcac cagcaggcac ttggtttgct
     2641 ggtaatgctc cagcatcatc ttgtgggttt cgcgaccgat acctgattgt ttgtagccac
     2701 caaacgcagc atgtgccgga taagcgtgat aacagttggt ccacacgcgc ccagcctgta
     2761 tgccgcgccc catcttatag gccagattac cgttgcggct ccagacgccc gcgcccaggc
     2821 catattgcgt atcgttcgcc agctccagcg cttcttccat cgttttgaag gtggtcaccg
     2881 ccagcaccgg gccaaaaatc tcctcctgga acacccgcat attgttctga ccaaacagaa
     2941 tcgtcggttc gaggtagtag ccgtctttca gttcaccttc cagcagcttg cgccgcccgc
     3001 ctgtgagcac gtcagcgccc tcttttttac cgatatcaat gtagttgagg atggtttcca
     3061 gttgcccgtg agaaacctgc gcgcccattt gcgtcacgct gtcgagcggg ttaccgctgc
     3121 gaatgctttc gacacggcgg atggcgcgtt ccataaagcg ttcgtagatt gactcctgca
     3181 ctaaggcacg actcggacag gtgcaaactt cgccctggtt aaaggcaaac agtgcaaagc
     3241 cttccagcgc tttatcgaaa aaggcatctt cttcatccat cacatcagca aagaagatat
     3301 ttggcgactt accgcccaac tccagcgtca ccggaataat gttttgcgtt gcgtattgca
     3361 taatttgttg gcccacttcc gttgagccgg taaacgccac tttggcgatg cgtttcgagg
     3421 tcgccagata ttcgccaatt tccccacctg cgccattgac cacgttcacc acgcccggcg
     3481 gcagtaaatc accgacaatt tccattagca gcagtacaga aagcggggta agacgtgcgg
     3541 gtttcagcac cacacagttg cccgccgcca gcgcgggagc cattttccag ctcgccatca
     3601 gcagcgggaa gttccacggg ataatctgcc ccaccacgcc taacggttca tggaaatgat
     3661 aggccacggt ttcgctatca acttcactga tcccaccttc ctgcgcccga atacacgagg
     3721 cgaaatagcg gaaatggtca atcgccagcg gtacatccgc agcactggtt tcgcgaatgg
     3781 gtttgccgtt atcccaggtt tcagctgtcg ctaacagctc gaggttttgt tccattcgat
     3841 cggcaatctt aaacagaatc gccgcgcgat cctgcaccga ggtgtgcgcc catttatctt
     3901 tcactttgtg cgcagcatcc agcgccagat cgatgtctcg tttgcccgaa gacgccactt
     3961 cgcacagcag ctgcccggtc accggcgtca gattctggta atactcacca tcggcagggg
     4021 ctacccattc accgccaata aagttgtcgt agcgggcttt taacttgatg gggaaaccat
     4081 actcgccggg cttaatctgt gctgaagggg gattattggt catgatcgtc tccttgcggt
     4141 gtgaggtata acaagggtag acgtgactgg cgaaatcttc gccagtcggt aacagcttta
     4201 cgacagctat cacgaattta cgggcaagga ttacagagat agttgaaaaa gcggctgaca
     4261 atttgccagc cgttgttgga atgatgatta cattgcggcg cgataaatca ccacaatttc
     4321 ttcgtgagtt gcctggatcg ggttagtaaa gccacaggca tctttcaggg cattagtcgc
     4381 cagaaccgcg aaatcttctt ctttcacgtt caggtcgcgt aggcctgccg ggatatccac
     4441 tttcttcgcc agttcacgga tggcgttaat gcaggcttca gcaccttccg cgtcgttttt
     4501 acctgtcacg ttcacgccca ttgcagcggc acagtcacgc agacgtgcag cggcgacttt
     4561 gctgttgaat acctgaacat gcggcagcag aacggcgtta catacaccgt gtggcaggtt
     4621 gtagaaaccg cccagctggt gcgccatcgc atgaacataa cccagagaag cattattgaa
     4681 cgccataccg gcaaggaact gggcataagc cattgcttca cgcgctttcg cattactgcc
     4741 atcttcaacg gctaacggca ggttttcggc aatcatggtc acggctttca gtgcacaagc
     4801 gtcagtgatc ggcgtggcgg caatagaaac atacgcttcg atagcgtgcg ttaaggcatc
     4861 catacccgtt gcggcggtca gtgacttcgg cataccaatc atcagagagg agtcattgac
     4921 agaaagcagc ggagtcacgt gcttatcaac aatcgccatt ttgttatgac gcgcttcgtc
     4981 agtgatgatg cagaaacggg tcatttcaga ggccgtaccc gccgtggtat tgatggcgat
     5041 catcggcagc tgcggttttg cagagcggtc aacgccttcg taatcgcgaa tatcgccgcc
     5101 attggctgcc accagcgcaa taccttttgc gcagtcgtgt ggagaaccac cgcctaagga
     5161 gatcacgcta tcgcaattat tttctttaag taatttcaaa cctgcggcga cgttttccgt
     5221 ggtggggtta ggttgggtgc catcataaat aacgctaaaa atattgcgtt cttccagtgc
     5281 tttttgcaca tcacccgcca tacctaattt cgttaacata ttgtcagtga caattaaggt
     5341 acgggtaaat ccataatctg ccatcatatt cattgcatca gtcaatgaat cagcgccgat
     5401 gacattcaca gaaggaataa agaaagttga agatgccata acacactcct gaaagtgtaa
     5461 aaaagggtgg gcagcttact gaggattttc attcgaaaac atgatcatta ttattaatta
     5521 tcaaaattaa tctaataaaa agcacattgt attcattaaa caatgtgctt tatttaaaat
     5581 cataagaaat ttgtattaat ttattttaaa aattaattaa tcatttcctt atttttccgg
     5641 aaataataat gcgtcgcgca ataaatgatc atttccacga cgacgcgtaa agccaatgcg
     5701 gtcaaaatat tccagaatct gaattgccag ctttcggcct acgcctaagc gatcgcggaa
     5761 atccgccgcg caggttgaac cacactcctg atcgagatcg cggatcatat tggcaaattc
     5821 gacaatccga tcgttacggt aataacgatc tttgacgatc gcggtaatta ttccttgctg
     5881 cgccgcctgg cgtagcgtca ggcgcattgc ctgctcgtcg gtacccgtct cttttgccag
     5941 atcacgcacc caccacggct cgtcaccaaa cagcggctct gctttttgcc agatggcctg
     6001 ctgctcttcg ctgaagcccg ctttgtgatc cggcagatgc agccaaccgt gatgactgag
     6061 gatgtcgccg ctttcgcgca tcttttcaat cagcaacagt actagcgctt catcttccat
     6121 tggcaacgcc atacgtcgca gacgttcgcg cccaggccca ggttcatcgc gatgttgctc
     6181 atgataagtc gctaatgtgt cgagaatttt ccgttgccag cgagcggcaa ccggcgcatt
     6241 caacaagcta taaccagcct gaatatagcc aggctgttgc agcagttcgc gcatcccttc
     6301 gccgttgagc tggcgcgccc aggcgaaatc aggaagatta accgcgccgc gttgcaggtg
     6361 aacggctaac gcttcgctat cattctgtgc cctggcgagg gaagccagcc attgcagata
     6421 ctctggttta cgtttaccgc gacgcggcgg gttaagcatc acgacgcgcg ctccggccag
     6481 cgtgttgcgg gcagagatat cgcgcaatac caggcggtcg ttatctgcca gccataacgg
     6541 ggtgtcgaag accagttcag caaggttatc ttccagcagt gaaacgcgtc ccgtgacgtg
     6601 gctggcggcg tggtgaatat gcagcggctg ccactgggtc agcggtgcat gggtttgaag
     6661 ctcgacaatc acccgtgtga acggctctgg cggcacatcg gcaagcagcc agtcgccacg
     6721 gttaatctgc tctttttccg catcacccgc gatgtcaagc gcgatacgct gcccggcatt
     6781 ggcggtttct gttggctggt tttgcgcatg cagcgcacgt acacgcatcg gtttatttac
     6841 accagtcagc cagagtgaat cgcctacctt cacttccccg cttaacgccg taccggtgac
     6901 gaccagcccg gcacctttta cggtaaatgc gcggtcaatc gcgaggcgga aactatgttg
     6961 gctggcgtgc tcgcgttccg gcaactgaag cagatgctcg cgcagggcat caattccccg
     7021 accttcggtt gctgcggtga taaacagttt tgcctcagca aaaccgtatt cccgcagaac
     7081 ctcctttacc tggcgttcaa cctcatcaac acgcgcttcg tccacgcgat cggctttggt
     7141 cagcgccact gtcagcatcg ggttaccggt cagctgcaaa atcgccagat gctcacgggt
     7201 ctgtgccatc acgccgtcat cgcacgccac caccaacagc gcgtgatcga taccaccaac
     7261 gcccgccagc atgttggaaa gaaacttttc atgaccggga acgtcgataa aaccaggcac
     7321 gcgaccatcc ggctgcggcc agtaggcata gccgagatcg atggtcatgc cgcgcttttt
     7381 ttcttccggc agacggtcag catttacgcc agtaatcgcc tgcaataagg ttgttttgcc
     7441 gtggtcaacg tgtccggcag tcgcaataat catttcaaca acatctccaa aaaccgttgc
     7501 tcatcttcaa ggcagcgtaa atccagccac aatcgtccgt cataaatacg accaatcacc
     7561 ggcactggca attcacgcca gcgggcggct aatgactcaa ggtggctacc gcgtccatca
     7621 tggggtgtaa acgttaatgc cgcgctcggc aggcgatcaa ccggcagcga accactgcca
     7681 atctgcgaaa gacatggcat aacctgtacc gcaaactccg cgccgtaatg tgcgacaagg
     7741 ggagcctgta aacgttgtgc ctggatttga atgacctctg cgctgcgggt aagcaggcgc
     7801 agggtcggta atttttcact cagagcttca gggtgtaaat aaagacgcaa cgtggcttcc
     7861 agcgccgcga gggtcatttt atccgcgcgt aatgcacgct tcagcgggtg gctttgcagg
     7921 cgggcgatca tctctttttt accaacgata atcccggcct gcggcccgcc taacaacttg
     7981 tcgccggaga agctcaccag actgacgccc gccgcaatca actcctgcgg cattggctct
     8041 ttcggcaaac cgtactggct aagatcgacc agcgagccac tgcctaaatc agtcaccact
     8101 ggaacatcca gctctttgcc gagcgccacc agttcagctt cgtctatcgc tttggtgaac
     8161 ccctgaatgc tgtagttact ggtatgtact ttcatcaaca gtgcggtatt ttcattcacc
     8221 gcctgacgat aatcattcgc gtgcgtgcgg ttggtggtcc ctacttcgtg tagggtgcag
     8281 cctgcctgac gcataacatc gggaatacga aacgcgccgc caatctccac cagttcgccg
     8341 cgagatacca ccacctcttt cccgctggca gtggccgcca acatcaataa caccgccgcc
     8401 gcattgttat tgacgataca ggcatcttcc gcccccgtaa tacggcacag cagctgcgcc
     8461 agcgcccgat cgcgatgtcc gcgtccggcg tcgtccagat cgtactcgag ggtcactggc
     8521 gaacgcatag cctgcgcaac ggcttccacc gcggcttccg cctgtaaagc tcgcccaagg
     8581 ttggtatgca gcacggttcc cgtcaggttg atcaccggac gcagcgcgct ctgcgcctct
     8641 ttcgtcaacc gggcatcgac ttcttgcgcc cagttttcac accacgcagg cagcgtctgg
     8701 ctgccacgaa tcacttctcg cgcttcgtcg agcatctgac gcaacaattc caccacgcgg
     8761 gtgtgaccat aagtatcacg caaagaaagg aaggagctat cgcgcaataa gcgatcaata
     8821 gccggaagtt gactatagag ggaacgcgtt tcggttgtca taggaaacct ggctgatcaa
     8881 ggccctctca cacggagaag ggcgtttaac ataaccacgg attgtaacgt gagatgggtc
     8941 aggaggacat atcgcgcatc aagcctttgg cggttcggtg cgagcaaaac tttcgcggct
     9001 aaacaggttt tcgaccagtt tgactaagtg tgggcggtcg acacaccaac ctggcgcaac
     9061 gcggcggaaa ttgagatatc cgacagcaca ggcaatggcg atagtcgcca gattgaccgt
     9121 atcggttttg agtgtgccat cgaccagata tccttccagc acatccagac tgcggttgat
     9181 tttctcccgc tggcgtaaca attcatcttc agactgctgc gccgccggac gcgcctgttc
     9241 acgcaccgat accagcccgg catccataat gccatccgcc agcgcctcaa ttttgcgcac
     9301 ccgcaacgac tccagcggat cgcgcggcaa catcgccgga gcgacattca ttaactcaat
     9361 atattcagcg atgatcggcg aatcaaacca gcattcgccc tcttcggtca ccagcaccgg
     9421 cactttccct aacgggttaa attgcgccac gccgttatcc gcgttatagg gcagttcatt
     9481 aataaattcg aaagttatgc ccttttctaa caacagaata gaaagtttgc gtacaaacgg
     9541 gctggtgtag ctaccgacga gtttcatgcc gagtcctttg tgcgaggaaa aatatcagta
     9601 tggctcaggc tcacgcaaaa cacaggtgat ataagtctca atgatccaaa taaagataat
     9661 gcatccaact ggtcattctt agcagcactt tccgcatcac cgaaacatgg ctgaaatggt
     9721 cggagtaaac cgccacctgg gcgtagatgc cgtcgggtaa ggcatcgata tcatcgttag
     9781 ggtccagttc aatggttccc agcacaccgt ccgtgccggg aacgaccgtt aatgattgca
     9841 ataccccctg cgcctgataa gaaccgcctg gcacgacagg taaaatacta gtcaatttgc
     9901 cgtggaacac ctgcccaggt agcgcgttaa acaccacttc cgcatcatcg ccaggtttca
     9961 gacgtaacag cgagttttgc cgaaattggg cgacaatttg ccgtttttgc tcggggatga
    10021 agaccatcac cggacgcagc ggcaaggcag ctgcgtatgt acctgggcgg atcagtacct
    10081 gagtgacgta gccattgctt ggtgcgcgaa tgacagtctg ctcaagatta tattttgctt
    10141 cagtaagttg cgctcttaag ctcacaatct gcgattgctc gccgttgacc atactgtcga
    10201 gctgactctg gatctgcgcc tgctccgcca ccgagccttt cactaacgca tcctgcgcga
    10261 ggaaattttg ccgcgcatcg tcgatgtcac gttccgagaa cgaattcacc gccgcctggc
    10321 tgcctttcag gtaacgttga taatttttaa acagacggtc gcgctccgct gaaacctggg
    10381 tggtgttggc ctgcgcttca gtgagctgcg cacgcagcgt ctttatatta tgcgtcgccg
    10441 tcatcaggtc agcctgaagt ctgtcaactc gcgcctggta acgaaccggg tcgagcttaa
    10501 aaagcacctc gcccttttga ataagctgat tattcttgtc agtgacttca gtaacaattc
    10561 ccgtcacctg tggcgtgata gggatcgcta tcactgcctt ttgcgcggta aaagtgtaag
    10621 ggtggttgta gttcatcaac aaaatcaaac cactcaccag aaacacgcct cccagcgccg
    10681 ccgtcgccag cgccacgtaa gttaaaacaa tcaatagatc cataattaga tctccgggaa
    10741 cgtggaatag tcagtctttt tctcggcggc gagttgatgc tccagccggg aaattcggtc
    10801 agaaagtgcg gcgatttccg gttcagtcgc tttctcctgc gacgcaacat gtgactgcat
    10861 accccagcca cgctccggtt gatagagcgt cgcccagatc cacagaaacg gccagataac
    10921 atgcagagta aacaggctca cccagcccgc cgtatgaata gcgtcggcat gggggtgatt
    10981 gcgctttttg gcaatcagat aagggatgtc atgtatcgcg atgattccat aaaaaatcac
    11041 cagaaagacg aagatcagca ctcccaacgc gaaatagttt agaaacatat ctgcctcgga
    11101 ttcacgttta tcagtgttgt tttttggctg gcagccagaa gggagtcagg ctgatatttt
    11161 gacaataatc cgggttcgcg attctcgcca taacaccaaa gaataatttt tagaggtgat
    11221 gagttgctta gttacataac gattgtatga cgaaggcata acatgctgta gatcacatca
    11281 ggtgaacgcc gtaagaaaat atcttgtgat tcagatcaca aagattcaac aaaccatcaa
    11341 aacaaaaatg tgacattact cacatttaaa tgccattttt agcgaaaatc accgccttgt
    11401 ttctttttta tacaggcgtt ttgtgatgag cgtcacgtca attacctctc taccccctat
    11461 atttatgtga ctgatatcac acaaaagccc gtcgactgga cagttaaccg attcagtgcc
    11521 agatttcgca gtatctacaa ggtccggcta cctctgccgc cacattaaca aaaaacctcg
    11581 ggcttccagc ctgcgcgaca gcaaacataa gaaggggtgt ttttatgtca tccgatatta
    11641 agatcaaagt gcaaagcttt ggtcgtttcc tcagcaacat ggtgatgcca aatatcggcg
    11701 cgtttatcgc gtggggtatc atcaccgcgt tatttattcc aacagggtgg ttaccgaacg
    11761 agacgctggc gaagctggtc gggccgatga tcacttatct cctaccgctg ctgatcggtt
    11821 ataccggtgg taagctggta ggcggcaaac gtggtggcgt agtcggtgcc attaccacca
    11881 tgggcgttat cgtcggcgca gacatgccga tgttcctcgg ttctatgatt gcaggtccgc
    11941 tgggcggctg gtgcattaag cacttcgacc gctgggtaga cggtaagatc aaatccggtt
    12001 ttgagatgct ggtgaataac ttctccgcag gcatcatcgg gatgatcctc gctattctgg
    12061 cattcctcgg cattggcccg attgttgaag ccctgtccaa aatgctggct gcgggcgtta
    12121 acttcatggt tgtccatgac atgctgccgc tggcgtctat ctttgttgaa ccggcgaaaa
    12181 tcctgttcct caacaacgcc attaaccacg gtatcttctc gccgctgggt attcagcagt
    12241 cccatgaact gggtaaatca atcttcttcc tgattgaagc taacccaggt ccaggtatgg
    12301 gcgtgctgct ggcgtacatg ttctttggtc gtggtagcgc taaacagtct gcgggcggtg
    12361 cggcaatcat ccacttcctg ggtggtatcc acgaaatcta cttcccgtat gtgctgatga
    12421 atccgcgtct gatcctcgca gtcatcctcg gcggtatgac tggcgtgttc acgctgacta
    12481 tcctgggcgg tggtctggtt tctccggcat ctccgggttc tatccttgct gtactggcga
    12541 tgacaccaaa aggtgcttat ttcgctaaca tcgcgggtgt gtgtgcggcg atggctgtct
    12601 ccttcgttgt ctctgctatt ttgctgaaaa ccagcaaagt gaaagaagaa gatgatattg
    12661 aagcagcaac tcgtcgtatg caggacatga aagctgagtc taaaggcaca tctccgctat
    12721 ctgcgggcga tgtgactaac gacctgagcc acgtacgtaa aatcatcgtt gcctgtgacg
    12781 ccggtatggg ttcaagtgcg atgggcgcag gcgtgctgcg taagaaaatt caggatgcag
    12841 gtctgtcgca gatttcggtc actaacagcg cgatcaacaa cctgccgcca gatgtggacc
    12901 tcgtcatcac tcaccgtgac ctgaccgaac gcgctatgcg ccaggttccg caggcacagc
    12961 atatttcgct gaccaacttc ctcgacagcg gcctgtacac cagcctaacc gaacgtctcg
    13021 ttgctgccca gcgccatact gaaaacgaag tgaaagtgaa agacagtctg aaagacagct
    13081 ttgacgattc cagtgctaac ctgttcaaac tgggcgcgga gaacatcttc ctcggtcgca
    13141 aagcagcaac caaagaagaa gcgattcgtt ttgctggcga gcagctggtg aaaggcggtt
    13201 acgttgagcc ggaatacgtt caggcgatgc tggatcgtga aaaactgacc ccgacttatc
    13261 tgggtgagtc tatcgcggtg ccacacggta cggttgaagc gaaagatcgc gtactgaaaa
    13321 cgggcgtggt gttctgccag tacccggaag gcgtgcgctt cggtgaagaa gaagatgaca
    13381 ttgcccgtct ggtgattggt attgcagccc gtaacaacga gcacattcag gttatcacca
    13441 gcctgaccaa tgcactggat gatgagtccg tcatcgagcg tctggcacac accaccagcg
    13501 tggatgaagt gctggaactg ctggcaggtc gtaagtaatc caatcctacc ctctcctcac
    13561 ggagagggtt tgggtgaggg aaaagcctca ccccagccct ctcgggtaaa aacattgatg
    13621 aaggttaata ctatgaaagc attacatttt ggcgcaggta atatcggtcg tggctttatc
    13681 ggtaaactgc tggcagacgc gggtatccaa ctgacgtttg ccgatgtcaa tcaggtggta
    13741 cttgatgccc tgaatgcccg tcatagctat caggttcatg tggtcggtga aaccgagcag
    13801 gtagataccg tttccggcgt caatgctgtc agcagcattg gtgatgatgt cgttgatctg
    13861 attgctcagg tcgatttagt cactaccgcc gttggcccgg ttgtgctgga acgtattgct
    13921 ccggctatcg ccaaagggct ggtgaaacgc aaagaacaag gtaatgaatc cccgctgaac
    13981 atcatcgcct gtgaaaacat ggtacgcggc accacgcagc tgaaaggtca tgtgatgaac
    14041 gccctgccgg aagacgccaa agcgtgggta gaagagcacg ttggctttgt cgattccgcc
    14101 gttgaccgca tcgtaccgcc ttcggcttcg gcaactaacg atccgctgga agtgacggta
    14161 gaaactttca gcgaatggat tgtcgataaa acgcagttca aaggcgcact gccgaacatc
    14221 ccaggcatgg agttaaccga caacctgatg gcatttgtcg aacgtaaact cttcaccctg
    14281 aacacgggtc atgctataac cgcgtacctc ggaaaactgg ccggtcatca gaccattcgt
    14341 gacgctattc tcgacgagaa aatccgcgcg gtggtaaaag gtgcgatgga agaaagtggt
    14401 gcggtactga tcaagcgcta cggctttgac gcagacaaac atgcggcgta catccagaaa
    14461 atcctcggtc gttttgagaa cccgtatctg aaagatgatg tagagcgcgt aggccgtcag
    14521 ccgctgcgta aactgagtgc tggcgaccgt ctgatcaagc cactgctcgg tacgctggaa
    14581 tacagccttc cgcacaagaa tctgattcag gggattgctg gtgcaatgca cttccgcagt
    14641 gaagatgatc cgcaggctca ggaactggca gcactgatcg ctgacaaagg tccgcaggcg
    14701 gcgctggcac agatttccgg tcttgatgcc aacagcgagg ttgtatccga ggcggtaacc
    14761 gcttataaag caatgcaata atggtggacc aggcgcagga caccctgcgc ccgaataaca
    14821 gattgtcaga tatgcaggca acaatggaac aaacccaggc cgttgaaaac cgtgtgcttg
    14881 agcgtctgaa tgctggcaaa accgtgcgaa gctttctgat caccgccgtc gagcttttga
    14941 ccgaggcggt aaatcttctg gtgcttcagg tattccgcaa agacgattac gcggtgaagt
    15001 atgctgtaga accgttactc gacggcgacg gtccgctggg cgatctttct gtgcgtttaa
    15061 aactcattta cgggttgggc gtcattaacc gccaggaata cgaagatgcg gaactgctga
    15121 tggcattgcg tgaagagcta aatcacgacg gcaacgagta cgcctttacc gacgacgaaa
    15181 tccttggacc ctttggtgaa ctgcactgcg tggcggcgtt accaccgccg ccacagtttg
    15241 aaccagcaga ctccagtttg tatgcaatgc aaattcagcg ctatcaacag gctgtgcgat
    15301 caacaatggt cctttcactg actgagctga tttccaaaat cagcttaaaa aaagcctttc
    15361 aaaagtaagc aacgtctgct tactgcccct ctacctgctt cggccgataa agccgacgat
    15421 aatactccag acgttgtaga tataacggca cgctttcatc gggtattccg gacggaatcg
    15481 cgttacgggg aggaagtttt ttcagatact cccggaacgc ctggcttgat gccatgaaat
    15541 ctacggcttt atcgataaga agcggaacgt tttcacctaa tttgcccatg atgatatctc
    15601 cgtattaccc cgcgccggga atgcgcggcc tccaatttta gtttaggttc ccggtaaaga
    15661 tgacattaag gaaaacgtgc tgaatcctca ataatcatcc gctgctgttt attatctttt
    15721 tctatagatt tcttaattaa tcaacgaatt gcgtagtttt tgagcatgtt aatgattacg
    15781 ttgcagaact attacaatgt catcagttgt gaacaaagca cctggtcgcg catactaggg
    15841 gctataaatt tatctttatc agaagccatc acatgaaaga agtcgaaaaa aacgaaatca
    15901 agcgtctcag cgatcgcctg gacgccatcc gccaccagca ggccgatctg tcgctggttg
    15961 aagccgcaga caaatatgcc gagctggaaa aagagaaagc cacgctggaa gcagaaattg
    16021 ctcgcctgcg tgaggttcat agccaaaaac tgagtaaaga agcacagaag ctgatgaaga
    16081 tgccgttcca gcgcgcaatt accaaaaaag agcaggctga tatgggcaag ctgaagaaaa
    16141 gtgttcgcgg actggtggtt gtgcacccaa tgaccgcgct ggggcgcgaa atgggcctgg
    16201 aagagatgac tggattttct aagactacgt tttaagcccc tcagacgact ggttcgatcg
    16261 tatcaacccg catctggctt gccttagctg gtcaatgcgg ggactctcct gtctactcgc
    16321 ctaattacca aacaattcac tccctttact tcgatttacc gctggttgat attctgaaaa
    16381 tgcattttta ccgaaatagc catctataaa caataaaaat atatagaaga tatcatttgt
    16441 taattaacaa gcatttacgt ctcttttttt cggatttaat tgtgaaattt taatcatcat
    16501 attcacaaaa tcaattatct gtgaaatttt tcccagacat ggtttctttc atatttgaca
    16561 tttattaata atcatcatta gaatccacga aaaataatat attcatacaa acacagacat
    16621 cttttgaaac gaatgataca tttttatgat gaatgcttaa aacaatattt aatcgacaca
    16681 catcactgtc attaatgtaa agcaacatag ctcaccaata atacaatgga caactctacc
    16741 ggcattgatc gctggtcgga ggaaataagg atttttacat gaacttgaag aagatttttt
    16801 tcagtgctgt cactgtttca gttctctgcg cattaacggg atgtgactac attgaagaag
    16861 gcaagccaga aagtagttta ctgaaacaac aagaagaaca taataataaa attgtgctac
    16921 ttgaaaaaca acaagcacag ctaaagagtc aactcgaaac tattcaaaaa caacaaactg
    16981 gcataataaa cagtacaaag actttaactc acgtaattaa atccgttaaa gaccaacaaa
    17041 atacttttat ttttaccgag tttaacccag caaaaacgaa gtatttcatc ctgaacaacg
    17101 gttcggtggc tttagcgggt cgagtgttat ccattgacgc cacagagaat ggtagtgtta
    17161 ttcatatctc actggtcaac ttattaagta cacctatctc aaacattggt tttaatgcga
    17221 catggggtgg tgaaaaacct gtcgatgcca aagagtttgc ccggtggcaa caattacttt
    17281 tcaacacatc aatgaaatcc acattgaaat tattaccagg tcaatggcaa gacattaatt
    17341 tgaccctgaa gggtgtatcg cccaataacc tgggatatct gaagttagcc atcaacatgg
    17401 aaaatattca gttcgacaat cttccctctg ctgaaaatcg gcagaaaaga agcaaaaaat
    17461 aaatgcagtt agtacgctta taacaaggaa tagcgagcat cattaatgaa caaaatattt
    17521 aaagttatct ggaaccctgc gacagggaat tatactgtta ccagcgaaac ggcaaaaagc
    17581 cgtggcaaga aatctgggcg cagtaagctg ttaatttctg cgctggttgc gggtggaatg
    17641 ttgtcgtcgt ttggggcatt ggcgaatgcc gggaatgaca acggtcaggg tgttgattac
    17701 ggtagtggat cagctggcga cggctgggtt gctataggca aaggggcgaa agcaaatact
    17761 tttatgaaca ccagtggttc cagtactgct gtgggttatg acgctatagc tgaaggccaa
    17821 tatagctctg ccatcgggtc aaaaacccat gcgattggtg gtgcatcaat ggcctttggg
    17881 gttagtgcaa tatcagaagg cgatagaagt atagcactgg gtgcctcttc gtattcattg
    17941 ggccaatact caatggccct cggccgttat tcaaaagcat tgggtaaatt gtctattgct
    18001 atgggggact cttccaaagc ggaaggagca aacgccattg ccctggggcg tagcagtgta
    18061 gctagcggta ctgacagcct cgcatttggc agacaatcac ttgccagcgc agcgaacgct
    18121 attgcgatag gtgctgagac cgaagccgct gaaaatgcaa ctgctattgg caataatgcg
    18181 aaggcaaaag ggactaatag catggcaatg gggttcggaa gccttgccga taaagtcaat
    18241 actatcgcat taggaaatag cagccaggct ctggcagata atgcaatcgc cataggccag
    18301 ggcaacaaag ctgatggcgt ggatgccatc gctctgggta atggtagcca gtcgagaggc
    18361 ttaaacacca ttgccttagg cacagccagt aatgcaactg gtgataagag tcttgcgctt
    18421 ggtagtaata gcagtgccaa cggtattaac tctgtcgcgc tgggcgcaga ttccattgcg
    18481 gatttagaca ataccgtctc tgtcggcaat agttcattaa aacgcaagat cgttaatgtg
    18541 aaaaatggcg cgatcaagtc tgacagttac gatgccatta atggttcaca gctttatgcc
    18601 attagcgact cggtagcaaa aaaggcttgg aggaggggct gcagtagatg ttgatgacgg
    18661 tactgttaca gcaccaacct acaatttaaa aaatggtagc aaaaataacg taggggctgc
    18721 gctcgctgta cttgatgaaa acaccctgca atgggaccaa accaaaggca aatacagcgc
    18781 tgctcatggt actagtagcc caactgccag cgtaatcacc gatgttgcgg atggcacgat
    18841 ttcagcctcc agtaaggatg cggttaacgg ttcccaactg aaagctacca atgacgatgt
    18901 cgaagccaac accaccaata tcaccaacct gacggattcc gttggtgacc ttcaggctga
    18961 tgccctgctc tggaacgaaa ctaaaaaggc attcagtgca gctcacggcc aggataccac
    19021 cagcaaaatc accaacgtta aagatgccga cctgacggct gacagcactg atgctgttaa
    19081 cggctctcag ctgaaaacca ccaacgatgc tgtggcgacg aataccacca atatcgccaa
    19141 taacacttcc aatattgcca ctaacaccac caacatctct aacctgactg agacggtgac
    19201 taatcttggt gaggatgcgc tgaaatggga taaggacaat ggtgtattca cggcagctca
    19261 tggcaccgag accaccagca aaataaccaa cgttaaagat ggcgacctga cgactggcag
    19321 caccgatgcc gttaacggct ctcagctgaa aaccaccaac gatgccgtgg cgacgaatac
    19381 caccaatatc gccactaaca ccaccaacat ctctaatctg actgagacgg tgactaatct
    19441 tggtgaggat gcgctgaaat gggataagga caatggtgtc ttcactgcag ctcatggcaa
    19501 caataccgcc agcaaaatca ccaatatcct ggacggcaca gtcactgcaa ccagttccga
    19561 tgccattaac ggtagccagc tttatgactt aagcagcaat atcgccacct acttcggcgg
    19621 caatgcttct gtgaatactg acggtgtgtt taccggtcca acctacaaaa tcggtgaaac
    19681 aaattattat aacgtcggcg atgcactggc tgcgattaac tcctcattta gcacgtctct
    19741 cggcgatgct ctgctttggg atgccaccgc aggtaaattc agtgccaaac acggtactaa
    19801 tggtgacgca agcgtgatca ctgatgtcgc agatggtgaa atttcagact ccagttctga
    19861 cgcagtaaac ggctcacaac tccacggcgt gagcagttat gttgttgatg cgctgggggg
    19921 tggtgccgaa gtcaatgcag acggcaccat caccgcaccg acttacacca ttgcgaacgc
    19981 cgagtacaac aacgtcggtg acgccctgga tgcgcttgat gataacgccc tgctgtggga
    20041 taagactgcc aatggcggtg ctggagccta caatgccagc catgacggta aagccagcat
    20101 catcactaat gtcgctaatg gcagtattag tgaggacagt accgatgcag tgaacggttc
    20161 tcagttgaat gcgacgaata tgatgattga gcagaacacc caaattatca atcagctcgc
    20221 tggtaacacc gacgcaacct atatcgaaga aaatggcgct ggtatcaact acgttcgtac
    20281 taacgacaac gatttagcct ttaacgatgc aagcgcctct ggtgtcggcg ctacagctgt
    20341 aggttataac gctgtcgcgt ctggtgccag cagcgtagcc attggtcaga acagcagcag
    20401 caccgttgat accggtattg cgctgggtag cagctccgtt tccagccgtg tgattgccaa
    20461 aggttctcgt gacactagcg taacggaaaa tggcgtggtg attggttatg acaccactga
    20521 cggcgaactg ctaggtgcat tgtcaattgg tgatgacggt aaataccgcc aaatcatcaa
    20581 cgtagctgat ggttcagaag cccatgacgc cgttacggtt cgccagttgc agaacgctat
    20641 tggagcggtc gccactacgc caaccaagta ctttcacgcc aactcaacgg cagaagactc
    20701 actggccgtt ggtgaagact cgctggcaat gggtgcgaaa actgtcgtta atggtaatgc
    20761 aggtattggt atcggtttga acactctggt tctggctgat gcgatcaacg gcattgctat
    20821 cggcagtaac gcacgcgcaa accatgcaaa cagtatcgca atgggtaacg gttctcagac
    20881 cacccgtggt gcacagactg gctacaccgc ctacaacatg gacgcaccgc agaactctgt
    20941 aggtgagttt tctgtcggta gtgaagacgg tcaacgtcag atcacaaacg tcgcagctgg
    21001 ttcagcggat accgatgcgg ttaacgtggg tcagttgaaa gtcactgatg agcgcgtagc
    21061 gcaaaatacc cagagcatta ctaacctgaa caatcaggtc actaatctgg atactcgcgt
    21121 tactaatatc gaaaacggta ttggcgacat tgtcaccacc ggtagcacca agtacttcaa
    21181 gaccaacacc gatggcgtag atgccaacgc ccagggtaaa gatagcgttg ctattggttc
    21241 tggttccatt gctgccgctg acaacagcgt cgcactgggt accggttccg ttgcagagga
    21301 agaaaataca atctctgtag gttcttctac taaccaacgc cgtatcacca acgtagctgc
    21361 cagtgttaat gccaccgatg cggttaacgt gtcgcagctg aaatcttctg aagcaggcgg
    21421 agtgcgctac gacaccaaag ctgatggttc tatcgactat agcaatatca ccctcggtgg
    21481 tggcaacggc agtacgactc gtatcagcaa cgtctccgct ggcgtcaaca acaacgacgc
    21541 ggtgaactac gcgcagttga agcaaagcgc gcaggaaacg aagcaataca ccgatcagcg
    21601 gatggttgag atggataaca aactgtctaa aactgaaagc aagttgagcg gtggtatcgc
    21661 ttctgcaatg gcaatgaccg gtctgccgca ggcttataca ccgggtgcca gcatggcttc
    21721 tattggtggc ggtacttaca acggtgaatc ggcagttgct ttaggtgtat cgatggtgag
    21781 cgccaatggt cgttgggtct acaaattaca aggtagtacc aatagccagg gtgaatactc
    21841 cgccgcactc ggtgccggta ttcagtggta atgatccatt aagttagtgt gactaagggc
    21901 aaacgccaaa ttgcctgatg cgttacgcgc atcaggcaag ataaggcgct ccgggtcaac
    21961 aatctaaggg caattctctg atgaggattg cccttttctt taccagacat ctccccccac
    22021 aagaattggc cctaccaatt cttcgcttat ctgacctctg gttcacaatt ccccaattaa
    22081 aactcacatc aatattgcca atacataaca tttagttaac cattcattgt cattatccct
    22141 acacaacaca attggcagtg ccacttttac acaacgtgtg acaaggagat gagcaacaga
    22201 ctcattacac gatgtgcgtg gactctagga gacctgcaat gaatctctgg caacaaaact
    22261 acgatcccgc cgggaatatc tggctttcca gtctgatagc atcgcttccc atcctgtttt
    22321 tcttctttgc gctgattaag ctcaaactga aaggatacgt cgccgcctca tggacggtag
    22381 caatcgccct tgccgtggct ttgctgttct ataaaatgcc ggtcgctaac gcgctggcct
    22441 cagtggttta tggcttcttc tatggtttgt ggcccatcgc gtggatcatt attgcagcag
    22501 tgttcgtcta taagatctcg gtgaaaaccg ggcagtttga catcattcgc tcgtctattc
    22561 tttcgataac ccctgaccag cgtctgcaaa tgctgatcgt cggtttctgt ttcggcgctt
    22621 tccttgaagg agccgcaggc tttggcgcac cggtagcaat taccgccgca ttgctggtcg
    22681 gcctgggttt taaaccgctg tacgccgccg gactgtgcct gattgttaac accgcgccag
    22741 tggcatttgg cgcgatgggc attccaatcc tggttgccgg acaagtaaca ggcatcgaca
    22801 gctttgagat tggccagatg gtggggcgtc agctaccgtt tatgaccatt atcgtgctgt
    22861 tctggatcat ggcgattatg gacggctggc gcggtatcaa agagacgtgg cctgcggtcg
    22921 tggttgcagg cggctcgttt gccatcgctc agtaccttag ctctaacttc attgggccgg
    22981 agctgccgga cattatctct tcgctggtat cactgctctg cctgacgctg ttcctcaaac
    23041 gctggcaacc agttcgcgta ttccgcttcg gtgacttggg ggcgtcacag gttgatatga
    23101 cgctggccca caccggttac accgcgggtc aggtgttacg tgcctggaca ccgttcctgt
    23161 tcctgaccgc taccgtaaca ctgtggagca tcccgccgtt taaagccctg ttcgcatcgg
    23221 gtggtgcgct gtatgagtgg gtgatcaaca ttccggtgcc gtacctcaat aaactggttg
    23281 cccgtatgcc accagtggtc agcgaggcta cagcctatgc cgccgtgttt aagtttgact
    23341 ggttctctgc caccggcacc gccattctgt ttgctgcact gctctcgatt gtctggctga
    23401 agatgaaacc gtctgacgct atcagcacct tcggcagcac gctgaaagaa ctggctctac
    23461 ccatctactc catcggcatg gtgctggcgt tcgcctttat ctcgaactat tccggactgt
    23521 catcaacact ggcactggca ctggcgcaca ccggtcatgc attcaccttc ttctcgccgt
    23581 tcctcggctg gctgggggta ttcctgaccg ggtcggatac ctcatctaac gccctgttcg
    23641 ccgcgctgca agccaccgca gcacaacaaa ttggcgtctc tgatctgttg ctggttgccg
    23701 ccaataccac cggtggcgtc accggtaaga tgatctcccc gcaatctatc gctatcgcct
    23761 gtgcggcggt aggcctggtg ggcaaagagt ctgatttgtt ccgctttact gtcaaacaca
    23821 gcctgatctt cacctgtatg gtgggcgtga tcaccacgct tcaggcttat gtcttaacgt
    23881 ggatgattcc ttaatgattg ttttacccag acgcctgtca gacgaggttg ccgatcgtgt
    23941 gcgggcgctg attgatgaaa aaaacctgga agcgggcatg aagttgcccg ctgagcgcca
    24001 actggcgatg caactcggcg tatcacgtaa ttcactacgc gaggcgctgg caaaactggt
    24061 gagcgaaggc gtgctgctca gtcgacgcgg cggcgggacg tttattcgct ggcgtcatga
    24121 cacatggtcg gagcaaaaca tcgtccagcc gctaaaaacg ctgatggccg atgatccgga
    24181 ttacagtttc gatattctgg aagcccgcta cgccattgaa gccagcaccg catggcatgc
    24241 ggcaatgcgc gccacacctg gcgacaaaga aaagattcag ctttgctttg aagcaacgct
    24301 aagtgaagac ccggatatcg cctcacaagc ggacgttcgt tttcatctgg cgattgccga
    24361 agcctcacat aacatcgtgc tgctgcaaac catgcgcggt ttcttcgatg tcctgcaatc
    24421 ctcagtgaag catagccgtc agcggatgta tctggtgcca ccggtttttt cacaactgac
    24481 cgaacaacat caggctgtca ttgacgccat ttttgctggt gatgccgacg gggcgcgtaa
    24541 agcaatgatg gcgcacctta gttttgttca caccaccatg aaacgattcg atgaagatca
    24601 ggctcgccac gcacggatta cccgcctgcc cggtgagcat aatgagcatt cgagggagaa
    24661 aaacgcatga ttatttccgc agccagcgat tatcgcgccg cagcgcaacg cattctgccg
    24721 ccgttcctgt tccactatat ggatggtggt gcatattctg aatacacgct gcgccgcaac
    24781 gtggaagatt tgtcagaagt ggcactgcgc cagcgtattc tgaaaaacat gtccgactta
    24841 agcctggaaa cgacgctgtt taatgagaaa ttgtcgatgc cggtagcact ggcaccggtg
    24901 ggtttgtgtg gcatgtatgc gcgtcgtggc gaagttcagg cagccaaagc ggcggacgcg
    24961 catggcattc cgtttactct ctcgacggtt tccgtttgcc cgattgaaga agtcgcgcca
    25021 gccatcaagc gcccaatgtg gttccagctt tatgtactgc gcgatcgcgg ctttatgcgt
    25081 aacgcgctgg agcgagcaaa agcagcgggt tgttcgacgc tggttttcac cgtggatatg
    25141 ccgacaccgg gcgcacgcta ccgtgatgcg cattcaggta tgagcggccc aaatgcggca
    25201 atgcgccgct acttgcaggc ggtgacgcat ccgcaatggg cgtgggatgt gggcctgaac
    25261 ggtcgtccac atgatttagg taatatctca gcttatctcg gcaaaccgac cggactggaa
    25321 gattacatcg gctggctggg gaataacttc gatccgtcca tctcatggaa agaccttgag
    25381 tggatccgcg atttctggga tggcccgatg gtgatcaaag ggatcctcga tccggaagat
    25441 gcgcgcgatg cagtacgttt tggtgctgat ggaattgtgg tttctaacca cggtggccgc
    25501 cagctggacg gtgtactctc ttccgctcgt gcgttgcccg ctattgcaga tgcggtgaaa
    25561 ggtgatatcg ccattctggc ggatagcgga attcgtaacg ggcttgatgt cgtgcgtatg
    25621 attgcgctcg gtgccgacac catactgctg ggtcgtgctt tcctgtatgc gctggcaaca
    25681 gcgggccagg cgggtgtagc taacctgcta aatctgatcg aaaaagagat gaaagtggcg
    25741 atgacgctga ctggcgcgaa atcgatcagt gaaattacgc aagattcgct ggtgcagggg
    25801 ctgggtaaag agttgcctac ggcactggct ccgatggcga aagggaatgc ggcatagtcg
    25861 tttgcgcccc ctcaccctaa ccctctccct cagggagagg ggaccgttcg gcgctgtatg
    25921 tactccctca ctctgaaacg acaccgcact ctttttttct ccctcgcccc tccggggagg
    25981 gccggggtga ggggaaaagg ccgcacggtt caccaatttt ctgctatcct gcccccgcac
    26041 taagggggca gtatgctaaa catcgtactt tacgaaccag aaattccgcc aaatactggc
    26101 aacatcatcc gtctttgcgc taataccggc tttcgtctac atatcatcga accgatggga
    26161 tttgcctggg acgacaagcg cctgcgccgc gcggggctgg actatcacga gtttaccgcc
    26221 gttacgcgtc atcatgacta tcgcgcgttc ctcgaagcag aaaatcccca gcgcctgttt
    26281 gccctcacca cgaaaggtac gcctgctcac agcgccgtaa gctatcagga tggcgattat
    26341 ctgatgttcg gcccggaaac acgcggcctg ccagcgagca ttcttgatgc cctgcccgct
    26401 gaacaaaaaa ttcgcattcc gatggtgccg gacagccgca gcatgaatct gtccaatgcg
    26461 gtgtcggtag tggtgtatga agcctggcgg cagctgggat atccgggagc gttgttgaga
    26521 gattagttac tgtatgccgg atagatgatt acatcgcatc cggcacgatc acaggacatt
    26581 agatcccatc cccatactca aatgtatggt taataccgtt gaaatgctgg tccatatcca
    26641 ttgatggctt atcgctgtct ggtttaccga caatacgaac cggaacgcca gcggcggttg
    26701 tatgcggcgg caccggttgc agcaccacgg aacctgcgcc aatcttcgcg ccgcgaccaa
    26761 cttcaatatt gccgaggatt ttcgcgcccg cgccaatcat cacaccttca cgaattttcg
    26821 ggtgacggtc accaccagat ttacccgtac cgccaagcgt cacagattgc agaatcgata
    26881 cgtcgttttc aattaccgcc gtttcaccca caacgatgcc cgtggcgtgg tcgagcataa
    26941 tgccacggcc aatttttgct gccgggtgaa tatcgacctg gaacgtcaca gaaacctggt
    27001 tttgcagaaa gattgccaat gcgcgacgcc cctgattcca caaccagtga ccgatgcgat
    27061 aggcctgcaa ggcatgaaaa cccttcaggt ataacaacgg ggttgagtat ttatcgactg
    27121 ccgggtcgcg ggtacgcacc gcctgaatat cacaggccgc agaggcgatc atttccgggt
    27181 cagcggcgta ggcttcttca accacttcac ggatagcaat agcaggcata attggcgatg
    27241 acagcttgtt cgccagcatg tagctcagtg cactgccaag gttttcgtgc ttgagtagcg
    27301 tcgcgtggta aaaactggcc agcattggct cacagtccgc cagcgttctg gcttcggctt
    27361 taatattgtt ccagacaatt tccagttctt cacacgacat tgcttactcc acacgatgag
    27421 ataatgaccg gcccgttctg cgcgggccgg gtcatagcgg taacaaaggt tccctggggt
    27481 tagtgactgc tgcgctcgtc cttgcgtgca cgacctaata atgtcaatgc tgcctcgcgc
    27541 gcgttttttc cgcaatataa tacttgataa atttcctcgg ttattggcat ttcaacgccg
    27601 aagcgatgcg ccagttcgcg gacttctttc gtattgcggt agccttccac cacctgacca
    27661 atcttctcct gcgcgctttg tacatccatg ccctgaccga gcatcatacc aaaacggcgg
    27721 ttacgcgact ggttgtcggt acaggtaagc accagatcgc ccagccccgc catgcccata
    27781 aaggtggcag gatcggcacc cagcgccgca ccaagacgcg acatttcagc cagcccacgg
    27841 gtgatcagcg ccgtacgcgc attcgcacca aaaccgatac cgtcagacat ccccgcgcca
    27901 atggcaatga cgtttttcac cgcgccgcca agctgcacgc caatgaaatc cgggttgctg
    27961 taaacgcgga aacttttgcc gcagtgcagc agctgctgga gatcatcggc aaaggtctga
    28021 tcggtcgagg ccagcgaaat agctgtcggt aaacctgccg ccagttcttt cgcaaacgtt
    28081 gggccagaga taaccgccag cggaatttga tcgcctaagg cctcacgcgc cacgtcctgt
    28141 aacagacgtc cggtttccgc ttccagccct ttggtcgccc acaccagacg cgcatcagga
    28201 cgcatcagcg gtttaatctg gcgcagcact tcaccaaaga catggctggg tacgacgacg
    28261 agaatattac gactggctgc cagcgcagtg gcgagatcgc tttcaagatg gagcgtatcg
    28321 ggaaaaggca catcggggag aaacgcggcg ttacagcggt cgcgttcaag cgttgcgata
    28381 tgttcagggt catggcccca gaggacaacc tcgtggccat ttcttgccag ggtaatggca
    28441 agagcggtgc cgtacgagcc ggcaccgatc acagtcattg aagcattacg ttggttcatc
    28501 aggcatcctg atgttcttca gtaccttcgc cagcctgctg ctgcaaatag ttcatgaaca
    28561 gcgcatcgaa gttaaccggc gcaaggttca gttgcgggaa tgtaccgcgg gataccatgc
    28621 tggtgatgca ctcacgagca tacgggaaca gaatgttcgg gcagtatgcg cccaggcaat
    28681 gtgccatctg ggtgccttcg atacccgcga tggagaaaat accgccctgc tgaacttcac
    28741 acaggaacgc ggtttcttcg cccaaggagg ccgttacggt aacacgcagt accacttcgt
    28801 atacgtcatc tgccagttgg gaagatgccg tatccagatc aagtttaact tctggttgcc
    28861 aatctttctg gaaaacgtgc ggcgcgttcg gcgcttcgaa agagatatcc ttggtataaa
    28921 tacgttggat ctggaaagtc atttcagtgt tgttttgttc tgacatgtgt agaaaaccct
    28981 taagtgttgt ccttaaatac tgcgcaatgt aataccaacg ttcacacgtt atttcagcag
    29041 gggatccagt ccaccacgtg catccaatgc atacaagtca tcacagccgc caatgtgctg
    29101 tgcgtcaata aaaatctggg gaaccgtggt gcaaccgctg cgtttgatca tctcttcacg
    29161 cttggcggca ttgccatcga tcggcaactc ctggaaactc acgcccttgc tgctcagcag
    29221 tgcttttgca cgatggcaat acgggcaggt ttctttggta tagatttcaa cattggccat
    29281 aatttagctc ctgttttttt accctgaaat ttcatgttgc agggcggctg caaaatggag
    29341 tgtccccaga agcttataac aataagtcgc tgggggccta attgcagcta acggcctgca
    29401 tcatgaaaga cgacaggtaa attatttgcc gcgcaccaga ggcaagtttt cgccagccca
    29461 gccagcaacg ccctctttca gcacgaatac ttgcgcaaaa ccagctttcg tcagcgcgtt
    29521 tgcaggctcc tggcactgca tgccagaacc gtctaccacg ataaccgatt tgtctttgtg
    29581 cttctcaagc tcaccaacat tgttggcttt gatttcgctc ggcaacagat tgatagaacc
    29641 tgcgatatgg cctttacgga agtcatcacg ctgacgtaaa tccacaacca cagcgtcttc
    29701 tttgttgatc agacgcgtag cttcaccacg agtaatcacc ttcactttcg aggtcaggct
    29761 cttaaacgta gtcacaagaa ccgccaccag taacgcgatc caggcgatac tcagtatggg
    29821 atgacggcca acaaattgca taatttcttg catggggggt aacaactccc gacgtagtga
    29881 ttaaaaaaac caggaaagga gtatacctgc gcggtgcggc aaatacagcc agcgcgttaa
    29941 ctggaatgca attttgcggg gcacgacgaa aaaaaagcgc aaatctggca tcggcttgcc
    30001 gcgagcggtc gtatattttg atcttcagag gctattttat cgattcagct gtagtaaaat
    30061 tacgcaaatt ttgactcttg agtatgaggt tgtcgcaatg tcggtttcta aaaaacctat
    30121 ggtactggtg attctggatg gctatggcta tcgtgaagaa cagcaggata acgccatttt
    30181 tagtgctaaa accccggtaa tggatgcact gtgggccaat cgtccgcata ccctgatcga
    30241 cgcttccggt ctggaagtcg gtctgcctga ccgtcagatg ggtaactccg aagtaggcca
    30301 cgttaacctg ggtgccggcc gcatcgtgta tcaggacctg actcgtctgg acgttgaaat
    30361 caaagatcgc gctttctttg ctaatccagt gctggctggc gcggtacata aagcgaaaaa
    30421 cgcaggtaaa gcagtacaca ttatgggtct gctctccgca ggcggcgtac acagccacga
    30481 agatcacatc atggcgatgg tagaactggc agctgaacgc ggcgcagaaa aaatctacct
    30541 gcacgcattc cttgacggtc gcgacacccc gccgcgcagt gctgaatcct cgctgaaaaa
    30601 atttgaagag aaatttgctg cgctgggtaa aggccgcgta gcgtccatca ttggtcgtta
    30661 ctacgcgatg gaccgcgata accgttggga tcgcgttgaa aaagcctatg acctgctgac
    30721 tctggcgcag ggtgagttcc aggccgatac tgccgttgct ggtttgcagg cagcttatgc
    30781 tcgcgacgaa aacgacgagt tcgtgaaagc gaccgttatt cgtgcagaag gtcagccaga
    30841 tgcggcaatg gaagacggcg atgcgctgat tttcatgaac ttccgtgctg accgcgcgcg
    30901 tgaaatcact cgtgcattcg tgaacgctga tttcgatggc ttcgcgcgta agaaagtggt
    30961 taacgtcgat ttcgtgatgc tgaccgaata cgccgctgac atcaaaactg cggttgctta
    31021 cccacccgct tccctggtta acaccttcgg cgagtggatg gcgaaaaacg acaaaactca
    31081 gttgcgtatt tccgaaaccg aaaaatatgc ccacgttact ttcttcttca acggtggcgt
    31141 agaagagtcg ttcaaaggcg aagatcgcat tctgatcaac tcaccgaaag tggctaccta
    31201 cgatctgcaa ccggaaatga gctccgcaga gctgaccgaa aaactggttg cggccatcaa
    31261 gagcggcaaa tacgacacca ttatctgtaa ctatccgaac ggcgacatgg taggtcacac
    31321 cggggtgatg gaagcggcgg ttaaagcggt tgaagcactg gatcactgcg tggaagaagt
    31381 cgcgaaagcg gttgagtccg tgggtggaca actgctgatc accgctgacc acggtaacgc
    31441 tgaacagatg cgcgatccgg caacgggtca ggcacacacg gcacacacca acctgccagt
    31501 tccgctgatt tacgttggtg ataagaacgt gaaagcggtt gcaggcggca aactttctga
    31561 catcgcgccg accatgttgt cgctgatggg tatggaaatc ccgcaagaga tgactggtaa
    31621 gccgctgttc atcgtggaat aatccctccc catgagggga aaggcgatta ataccatgac
    31681 acgggccgtg aaaccgcgca ggtttgcaat caggcccatc atctacgcca gcgttcttag
    31741 cgctggcgta ttgttgtgcg ccttttccgc ccacgcggat gagcgtgacc aactcaaatc
    31801 tattcaggcc gatatcgccg caaaagagcg cgcggtacgc caaaagcaac aacaacgcgc
    31861 aagcctgctc gcacaattga aaaagcagga agaagcgatc tctgaagcca cccgtaagct
    31921 gcgcgaaacg caaaacacgc tcaatcaact caataaacag attgatgaga tgaacgcgtc
    31981 gattgccaaa ctggagcagc aaaaagccgc ccaggagcgc agcctcgccg cacaactgga
    32041 tgccgcattc cgtcagggcg agcataccgg tattcagcta attctcagcg gtgaagaaag
    32101 ccagcgtgga cagcgtttac aggcttattt cggctatctc aaccaggcgc gacaagaaac
    32161 cattgcccag ttgaagcaaa cgcgtgaaga agtcaccatg cagcgtgctg aactggaaga
    32221 gaaacaaagc gagcaacaaa cgctgttata tgaacagcgc gcccaacagg cgaaactgac
    32281 tcaggctctg aacgagcgta aaaagacgct ggcggggctg gaatcttcca tccagcaagg
    32341 tcagcagcag ctaagtgaac tgcgtgccaa cgaatcccgt ctgcgtaaca gcattgcccg
    32401 tgcggaagcc gcggcgaaag cgcgtgctga acgagaagcg cgtgaggccc aggcggttcg
    32461 cgagcgccag aaagaagcga cgcgcaaagg caccacctac aaaccgaccg aaagcgaaaa
    32521 atcgctgatg tcccgaactg gtggcctggg tgcgccgcgt ggtcaggcat tctggccggt
    32581 tcgcgggccg acgctgcatc gctatggcga gcagctacag ggtgaattac gctggaaagg
    32641 gatggttatc ggtgcctctg aaggtactga agttaaagca attgccgacg gtcgagtgat
    32701 tctggctgac tggctgcaag gttacggtct ggtggtggtg gttgagcacg gtaaaggcga
    32761 catgagtctt tacggctata atcagagcgc actggtgagc gttggttcgc aggttcgcgc
    32821 gggccagcca attgcactgg tgggcagcag tggcggtcag ggtcggcctt cactctattt
    32881 cgaaattcgc cgccagggtc aggcggtcaa tccacagccg tggttgggaa gataagtttt
    32941 gtttccattt cgtcgtaacg ttcttgcatt tgccgctctg ttggcgctct cctcccccgt
    33001 acttgctggc aaacttgcca tcgtcattga tgattttggg tatcgcccgc acaacgaaaa
    33061 ccaggtgctg gcgatgcctt ccgctatctc cgtcgctgta ttacccgatt caccgcacgc
    33121 cagagagatg gcgaccaaag cgcataacag cggtcacgaa gtgttgattc atctcccgat
    33181 ggcaccgttg agtaaacagc cgctggagaa aaatacgcta cgcccggaga tgagcagcga
    33241 cgaaattgag cgcattattc gtagtgcggt caataacgtg ccctatgccg tggggatcaa
    33301 caaccacatg ggcagcaaga tgacctctaa cctgtttggt atgcagaaag tgatgcaggc
    33361 gctggagcgt tacaatcttt acttcctcga cagcgtaacc atcggtaata cccaggcgat
    33421 gcgcgccgcg caaggtactg gcgtgaaggt gatcaaacgg aaggtgttcc tcgacgattc
    33481 gcagaacgaa gcggacatcc gtgtgcaatt taatcgcgca attgatctgg cgcgtcgcaa
    33541 cggttcaacc attgccattg ggcatcctca cccttcaacg gtacgcgtgc tacaacagat
    33601 ggtttataac ctgccgccag acattacgct ggtgaaagcc agcagcttgc tgaatgaacc
    33661 gcaggttgat acttctacac cgccgaaaaa cgctgtgcct gatgcaccgc gtaatccgtt
    33721 ccgtggcgtg aagctgtgca aaccgaagaa accgatagaa cctgtttatg ctaatcgctt
    33781 ctttgaagta ttaagcgaaa gcatcagcca gagcacgctg atcgtttact tccagcatca
    33841 gtggcaaggc tggggcaaac agcccgaagc ggcgaagttt aacgctagcg caaattaaag
    33901 gcgctacgcg taatgcgatg gtgcgaccgc gttttgtcgc gccattgcca taagcggaaa
    33961 gaccacagta acgcctggta accgaccttc acgctgcgca cattggtaat caggcgctta
    34021 tacatacccg gaagtaaata tctcggcaat catccgttgg cgggtaagaa tatccggctc
    34081 tttgcg
//
LOCUS       DAEEUE010000037        33692 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.037, whole genome shotgun sequence.
ACCESSION   DAEEUE010000037 DAEEUE010000000
VERSION     DAEEUE010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33692
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.037"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            78..1355
                     /gene="waaA"
                     /locus_tag="KLK84_001895"
     CDS             78..1355
                     /gene="waaA"
                     /locus_tag="KLK84_001895"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HBE2009240.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1363..1842
                     /gene="coaD"
                     /locus_tag="KLK84_001896"
     CDS             1363..1842
                     /gene="coaD"
                     /locus_tag="KLK84_001896"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBE2009241.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1881..2690)
                     /gene="mutM"
                     /locus_tag="KLK84_001897"
     CDS             complement(1881..2690)
                     /gene="mutM"
                     /locus_tag="KLK84_001897"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HBE2009242.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2788..2955)
                     /gene="rpmG"
                     /locus_tag="KLK84_001898"
     CDS             complement(2788..2955)
                     /gene="rpmG"
                     /locus_tag="KLK84_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBE2009243.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2976..3212)
                     /gene="rpmB"
                     /locus_tag="KLK84_001899"
     CDS             complement(2976..3212)
                     /gene="rpmB"
                     /locus_tag="KLK84_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBE2009244.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3429..4097)
                     /gene="radC"
                     /locus_tag="KLK84_001900"
     CDS             complement(3429..4097)
                     /gene="radC"
                     /locus_tag="KLK84_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBE2009245.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4269..5489
                     /gene="coaBC"
                     /locus_tag="KLK84_001901"
     CDS             4269..5489
                     /gene="coaBC"
                     /locus_tag="KLK84_001901"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBE2009246.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5467..5925
                     /gene="dut"
                     /locus_tag="KLK84_001902"
     CDS             5467..5925
                     /gene="dut"
                     /locus_tag="KLK84_001902"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBE2009247.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6032..6628
                     /gene="slmA"
                     /locus_tag="KLK84_001903"
     CDS             6032..6628
                     /gene="slmA"
                     /locus_tag="KLK84_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HBE2009248.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6665..7306)
                     /gene="pyrE"
                     /locus_tag="KLK84_001904"
     CDS             complement(6665..7306)
                     /gene="pyrE"
                     /locus_tag="KLK84_001904"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBE2009249.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7372..8088)
                     /gene="rph"
                     /locus_tag="KLK84_001905"
     CDS             complement(7372..8088)
                     /gene="rph"
                     /locus_tag="KLK84_001905"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBE2009250.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8215..9078
                     /locus_tag="KLK84_001906"
     CDS             8215..9078
                     /locus_tag="KLK84_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBE2009251.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9299..10123
                     /gene="dinD"
                     /locus_tag="KLK84_001907"
     CDS             9299..10123
                     /gene="dinD"
                     /locus_tag="KLK84_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HBE2009252.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10414..11031
                     /locus_tag="KLK84_001908"
     CDS             10414..11031
                     /locus_tag="KLK84_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HBE2009253.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11028..12710)
                     /gene="ligB"
                     /locus_tag="KLK84_001909"
     CDS             complement(11028..12710)
                     /gene="ligB"
                     /locus_tag="KLK84_001909"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HBE2009254.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12968..13591
                     /gene="gmk"
                     /locus_tag="KLK84_001910"
     CDS             12968..13591
                     /gene="gmk"
                     /locus_tag="KLK84_001910"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBE2009255.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13646..13921
                     /gene="rpoZ"
                     /locus_tag="KLK84_001911"
     CDS             13646..13921
                     /gene="rpoZ"
                     /locus_tag="KLK84_001911"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBE2009256.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13940..16048
                     /gene="spoT"
                     /locus_tag="KLK84_001912"
     CDS             13940..16048
                     /gene="spoT"
                     /locus_tag="KLK84_001912"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HBE2009257.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16055..16744
                     /gene="trmH"
                     /locus_tag="KLK84_001913"
     CDS             16055..16744
                     /gene="trmH"
                     /locus_tag="KLK84_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HBE2009258.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16750..18831
                     /gene="recG"
                     /locus_tag="KLK84_001914"
     CDS             16750..18831
                     /gene="recG"
                     /locus_tag="KLK84_001914"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBE2009259.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18865..20070)
                     /gene="gltS"
                     /locus_tag="KLK84_001915"
     CDS             complement(18865..20070)
                     /gene="gltS"
                     /locus_tag="KLK84_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HBE2009260.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20350..21741
                     /gene="xanP"
                     /locus_tag="KLK84_001916"
     CDS             20350..21741
                     /gene="xanP"
                     /locus_tag="KLK84_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HBE2009261.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21862..23571
                     /locus_tag="KLK84_001917"
     CDS             21862..23571
                     /locus_tag="KLK84_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBE2009262.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23624..25942)
                     /gene="yicI"
                     /locus_tag="KLK84_001918"
     CDS             complement(23624..25942)
                     /gene="yicI"
                     /locus_tag="KLK84_001918"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HBE2009263.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25952..27335)
                     /locus_tag="KLK84_001919"
                     /pseudo
     CDS             complement(25952..27335)
                     /locus_tag="KLK84_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27628..27722
                     /locus_tag="KLK84_001920"
     tRNA            27628..27722
                     /locus_tag="KLK84_001920"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27662..27664,aa:Sec,seq:tca)
     gene            28023..29207
                     /locus_tag="KLK84_001921"
     CDS             28023..29207
                     /locus_tag="KLK84_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE2009264.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPREAKKEEKIAQQMSLKNTFEA
                     IAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            29619..30662
                     /locus_tag="KLK84_001922"
     CDS             29619..30662
                     /locus_tag="KLK84_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBE2009265.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPENNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31177..31605
                     /locus_tag="KLK84_001923"
                     /pseudo
     CDS             31177..31605
                     /locus_tag="KLK84_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(31635..32786)
                     /locus_tag="KLK84_001924"
                     /pseudo
     CDS             complement(31635..32786)
                     /locus_tag="KLK84_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            32851..33505
                     /locus_tag="KLK84_001925"
                     /pseudo
     CDS             32851..33505
                     /locus_tag="KLK84_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         8436 a         8853 c         8298 g         8105 t
ORIGIN      
        1 aaaaacggga aaagtaatgg taaagccaca gctaaataca tagaatcccc agcacatcca
       61 taagtcagct atttactatg ctcgaattgc tttacaccgc ccttctctac cttattcagc
      121 cgctgatctg gatacggctc tgggtgcgcg gacgtaaggc tccggcctat cgaaaacgct
      181 ggggtgaacg ttacggtttt taccgccatc cgctaaaacc aggcggcatt atgctgcact
      241 ccgtctccgt cggtgaaact ctggcggcga tcccgttggt gcgcgcgctg cgtcatcgtt
      301 atcctgattt accgattacc gttacaacca tgacgccaac cggttcggag cgcgtacaat
      361 cggctttcgg gaaggatgtt cagcacgttt atctgccgta tgacctgccc gatgcgctca
      421 accgtttcct gaataaagtc gaccccaaac tggtgttgat tatggaaacc gaactatggc
      481 ctaacctgat tgcggcgcta cataaacgta aaattccgct ggtgatcgct aacgcgcgac
      541 tctctgcccg ctcggccgca ggttatgcca aactgggtaa attcgtccgc cgcttgctgc
      601 gtcgtattac gctgattgct gcgcaaaatg aagaagatgg tgcacgtttt gtggcgctgg
      661 gcgcaaaaaa taaccaggta accgttaccg gtagcctgaa attcgatatt tctgtaactc
      721 cgcagttggc tgctaaagcc gtgacgctac gccgccaatg ggcaccacac cgcccggtat
      781 ggattgccac cagcactcat gaaggtgaag agagcgtggt cattgccgca catcaggcat
      841 tgttacagca attcccgaat ttattgctca tcctggtacc ccgtcatcca gaacgcttcc
      901 cggatgcgat taaccttgtc cgccaggctg gactaagcta tatcacacgc tcttcagggg
      961 aagtcccctc caccagcacg caggttgtgg ttggcgatac gatgggcgag ttgatgttac
     1021 tgtacggcat tgccgatctc gcctttgttg gcggttcact ggttgaacgt ggtgggcata
     1081 atccgctgga agctgccgca cacgctattc cggtattgat ggggccgcat acttttaact
     1141 ttaaagacat ttgcgcgcgg ctggagcagg caagcgggct tattaccgtt accgatgcca
     1201 ctacgcttgc aaaagaggtt tcctctttac tcaccgacgc cgtttaccgt agtttctatg
     1261 gccgtcatgc cgttgaagta ctgtatcaaa accagggcgc gctacagcgt ctgcttcaac
     1321 tgctggaacc ttacctgcca ccgaaaacgc attgaggttg ttatgcaaaa acgggcgatt
     1381 tatccgggta ctttcgatcc cattaccaat ggtcatatcg atatcgtgac gcgcgccacg
     1441 cagatgttcg atcacgttat tctggcgatt gccgccagcc ccagtaaaaa accgatgttt
     1501 accctggaag agcgtgtggc actggcacag caggcaaccg cgcatctggg gaacgtggaa
     1561 gtggtcgggt ttagtgattt gatggcgaac ttcgcccgta atcaacacgc tacggtgctg
     1621 attcgtggcc tgcgtgcggt ggcggatttt gaatatgaaa tgcagctggc gcatatgaat
     1681 cgccacttaa tgccggaact ggaaagtgtg tttctgatgc cgtcgaaaga gtggtcgttt
     1741 atctcttcat cgttggtgaa agaggtggcg cgccatcagg gcgatgtcac ccatttcctg
     1801 ccggagaatg tccatcaggc gctgatggca aaattagcgt agcgtttatg ccggatggta
     1861 tgccatccgg ctcgcatgaa ttacttctgg cactgccgac aataaaacgt tgcccgctgc
     1921 gcatgtttag tcgccacaat cggcgtaccg cacacccgac acggctcacc ttttcgcccg
     1981 taaacctgca attcctgcgc gaaatagccc ggtttaccat cactttgcag aaaatctttc
     2041 agcgttgtac caccctgctc aatcgaacgc agcaacaccg ctttaatcac ccgagctaac
     2101 aattcacact ctgccagcga cagtgatgac gccagccgat ccggatggat ccccgccgca
     2161 aacagtgatt cgctggcata gatattccct acccctacca ccagcttgtt atccatcagc
     2221 cacggtttaa tcgccgtttt tttcttcgcg cacttctgat gcagatactc accattgaaa
     2281 tcgtcgctaa gcggctccgg tccaagatgg gccagcacat tatgcccttc cagctctttg
     2341 gtccacagcc aggcaccaaa gcggcgcgga tcggtgtagc gcagcacttt accgttgctc
     2401 atcaccaaat ccacatggtc atgcttttca gggggaagtt cttctggaag gatgcgcagg
     2461 ctgccagaca tccccaaatg aatgataatc cagccctcag gcagctccag cagcagatat
     2521 ttagcccgcc gctgcacgct aagcactggt tgatcgctta atcggtagat ctcttctgaa
     2581 accggccagc gcaagcgtcc gttgcgcacc accgcatgaa gaatggttgc accaacgaga
     2641 tgcggttcta tgccgcgacg gctggtttca acttcgggta attcaggcat agcatctcca
     2701 ggaatgaaca gatgcaatca atatgggggc aagcagataa caaaaaaccc cgccggagcg
     2761 aggtttttcg ttacatcaaa gcgagaatta tttgattttc gcttctttgt agatcacgtg
     2821 ctgacgaaca actggatcga attttttcag ttccagtttt tccggcttag tacgtttgtt
     2881 cttcgtagtg gtatagaagt gaccagtacc agcagaagaa accagcttga ttttctcacg
     2941 aataccttta gccatgattt atttcctcta agtacttagt acttttcgcc acgggcacgc
     3001 agttcagcca gaactgtatc gatgcctttt ttatcgatta cacgcatacc tttagcagat
     3061 acgcgcaggg tgacaaaacg cttctcgctc tcaacccaga aacggtgaga gtgcaggttc
     3121 ggcaggaaac ggcgtttagt cgcgttcagt gcgtgggaac ggttgttacc ggtcaccgga
     3181 cgcttgccag taacttggca gactcgggac atgtctattc tccaaaaatc aaattagctc
     3241 gagcttcgta tggggtattg gcgcctcgtc aggctttaca gcccggtcat cgcagttcta
     3301 tgtgaactct cgattgccag gcccaaatgc caaacccgag attctcaaag gtggcgtagt
     3361 atacgctgac tcagcgatgt gctcaagtcc cgaacagaca aagatcccga aggatcgcgc
     3421 atagcgggtt aaatccagcc gcgttcggca aaagaaacat actctccacg cccaatcacg
     3481 atatggtcga gcacgcgtaa atccatgaac tgacaactct ttattatccg ttcagtaatg
     3541 agtttatccg ctttactggg ttcagcacaa cccgaagggt gattatgtgc aaggatcagc
     3601 gccgaggcgt ttatttttat cgcttcgcgg ataatttccc gaggatggac ttcaacatgg
     3661 tttagcgtgc cggaaaaaag acggctatgc gttataaccc ggtgttgtga gtcgagaaag
     3721 atcaccataa agatctcccg ctcctcaccc gtgagctggc tttgtaaaaa ttcccgcgtc
     3781 atctccgggc tgagtaaagg gctttcttca cgcatccgca cgttgtagta acgtcgcgcc
     3841 agttcagcaa tcccctttaa ctgggcaaat ttcgctacgc caattccatg aacaccacta
     3901 aattgttcat attcagaggt taacaagcca taaagagagc cgaaattctc cagcatctct
     3961 tttgccaggg ttaatacatc tttaccgcgc gttccggtac gcagaaatag cgccagcagc
     4021 tcgacatccg ttaaggcgct aataccaaac ttcagcattt tttcgcgcgg catcaacagc
     4081 tgtgcattgt ttttcacctt cacctccttt gtggtgctcg catcctgtca tagccgttcc
     4141 gttaaatcga cggccagttt tcaatcctgg aaagagcctc gcaaagtgaa tctcaggtga
     4201 aggcacgaca acaaaagtat tgtgataaaa tcgccaactt ctggtgtcac ccaacaggaa
     4261 aaatcatcat gagcctggcc ggtaaaaaaa tcgttctcgg cgttagcggc ggtattgctg
     4321 cctataaaac ccctgaactg gtgcgtcgtt tgcgcgatcg cggggccgac gtccgcgtag
     4381 ccatgaccga agcggcaaaa gcctttatca ccccacttag cttgcaggcg gtttctggtt
     4441 atcccgtttc cgacagtctg cttgacccgg cagccgaagc cgctatgggc catattgagc
     4501 tgggtaaatg ggctgattta gtgattctcg cccctgccac ggcagatttg attgcccgtg
     4561 ttgctgccgg aatggcgaat gacctggtat cgacgatttg tctggctaca cctgcgcctg
     4621 tagccgtgct ccccgccatg aaccagcaga tgtaccgtgc cgctgccacg cagcataatt
     4681 tagaggtgct tgcttcccgt ggtttgctca tctgggggcc agacagtggc agtcaggctt
     4741 gtggtgatat cggtcctgga cgaatgctcg atccgttaac cattgtggat atggcggtgg
     4801 cgcatttttc gcccgtcaac gacctgaaac atctgaacat tatgattacc gccggtccga
     4861 cgcgtgaacc gctcgatccg gtgcgttata tctctaatca cagctccggc aagatgggtt
     4921 ttgctatcgc cgccgctgct gcccgtcgtg gcgcgaacgt cacgctggta tcaggtccag
     4981 tttcactacc gacgccaccg tttgttaaac gtgttgatgt gatgaccgcg ctggaaatgg
     5041 aagccgccgt gaatgcttct gtacagcagc aaaatatttt tatcggttgc gccgccgtgg
     5101 cggattatcg cgcagctacc gtggccccag aaaaaatcaa aaagcaggcc acgcagggtg
     5161 atgaattaac aataaaaatg gttaaaaacc ccgatatcgt cgcaggcgtt gccgcactaa
     5221 aagaccatcg accctacgtc gttggatttg ccgccgaaac aaataatgtg gaagaatacg
     5281 cccggcaaaa acgtatccgt aaaaaccttg atctgatctg cgcgaacgat gtttcccagc
     5341 caactcaagg atttaacagc gacaacaacg cattacacct tttctggcag gacggagata
     5401 aagtcttacc gcttgagcgc aaagagctcc ttggccaatt attactcgac gagatcgtga
     5461 cccgttatga tgaaaaaaat cgacgttaag attctggacc cgcgcgttag gaaggaattt
     5521 ccgctcccga cttatgccac ctctggctct gccggacttg acctgcgtgc ctgtctcgac
     5581 gacgccgtag aactggctcc gggtgacact acgctggttc cgaccgggct ggcgattcat
     5641 attgccgatc cttcactggc ggcaatgatg ctgccgcgct ccggattggg acataagcac
     5701 ggtatcgtgc ttggtaacct ggtaggattg atcgattccg actatcaggg ccagttgatg
     5761 atttccgtgt ggaaccgtgg tcaggacagc ttcaccattc aacctggcga acgcatcgcc
     5821 cagatgattt ttgttccggt agtacaggct gaatttaatc tggtggaaga tttcgacgcc
     5881 accgaccgcg gtgaaggcgg ctttggtcac tctggtcgtc agtaacacat acgcatccga
     5941 ataacgtcat aacatagccg caaacatttc gtttgcggtc atagcgtggg tgccgcctgg
     6001 caagtgctta ttttcagggg tattttgtaa catggcagaa aaacaaactg cgaaaaggaa
     6061 ccgtcgcgag gaaatacttc agtctctggc gctgatgctg gaatccagcg atggaagcca
     6121 acgtatcacg acggcaaaac tggccgcctc tgtcggcgtt tccgaagcgg cactgtatcg
     6181 ccacttcccc agtaagaccc gcatgttcga tagcctgatt gagtttatcg aagatagcct
     6241 gattactcgc atcaacctga ttctgaaaga tgagaaagac accacagcgc gcctgcgtct
     6301 gattgtgttg ctgcttctcg gttttggtga gcgtaatcct ggcctgaccc gcatcctcac
     6361 tggtcatgcg ctaatgtttg aacaggatcg cctgcaaggg cgcatcaacc agctgttcga
     6421 gcgtattgaa gcgcagctgc gccaggtatt gcgtgaaaag agaatgcgtg agggtgaagg
     6481 ttacaccacc gatgaaaccc tgctggcaag ccagatcctg gccttctgtg aaggtatgct
     6541 gtcacgtttt gtccgcagcg aatttaaata ccgcccgacg gatgattttg acgcccgctg
     6601 gccgctaatt gccgcgcagt tgcagtaata tgacgccgga tgacttttca tccggcgagt
     6661 ttctttaaac gccaaactct tcgcgatagg ccttaaccgc cgccagatgt tccgccattt
     6721 ccggcttctc ttccagatag gcaatcagat ctttcagggt gatgatagag atcactttgc
     6781 agttgtaatc acgctcaact tcctgaatcg ccgaaatctc gccgcgcccg cgttcctgac
     6841 gatcgagcga aatcaacacg ccagcaagcg tcgcgccatt ggcctgaata atctccatcg
     6901 actcgcgaat cgccgttccg gcggtgatca catcatctac cagcattacg cgtccttgta
     6961 acgcgctacc aaccagattg ccgccttcac cgtggtcttt tgcttctttg cggttaaagc
     7021 agtacggcag gtccaggtcg tgatgctccg ccagtgccac agcggttgtg gtggcaatcg
     7081 ggatcccttt gtaagcaggg ccaaacagca gatcgaactc aatgccggaa tccaccaacg
     7141 cttcagcgta aaaacggcct aacagtgcca gatcgcgccc ggtattaaac agcccggcgt
     7201 tgaagaaata ggggcttttg cgcccggatt tcagcgtaaa ctcgccaaac tttaacacct
     7261 gcttgctaag cgcaaattca ataaactggc gctgatatgg tttcatgcct tcgctcctca
     7321 tcttactttt ctacagacaa aaaaaaggcg actcatcagt cgccttaaaa atcagtttgc
     7381 cagcgccgcc ttctgcgtcg ctacaatgga ttcgattccc cctcgggcca gcgccaacaa
     7441 ggtgagtagc tcttcatggg tgaacggctc gccttctgcc gtcccctgca cttcaatgat
     7501 gcgcccgtct tcggtcatca ctacgttcat gtcggtctct gcggctgagt cttcaacgta
     7561 ttccagatcg caaattgctt caccgtttac aataccgaca gaaactgcgg ctaccatccc
     7621 tttcatcggg ttggttttca gcttgccgtt ttccaccagc ttctgtagcg catctgccag
     7681 cgccacgcag gcacccgtaa tcgacgcggt acgagtgcca ccatcagcct gaagcacgtc
     7741 gcagtccagc gtaatggtga actcacccag cgctttcaaa tctactgccg cgcgaagagc
     7801 acgggcgatc agacgctgga tttccattgt gcgtccaccc tgcttacctt tcgccgcttc
     7861 acgagcgtta cgggtgtggg tagaacgtgg cagcatgccg tactctgcgg tgatccagcc
     7921 ctggccctga cctttcagga agcgcggcac gccttcttca atagaggcgg tacacaatac
     7981 tttggtatcg ccaaattcga ccagcaccga gccttctgca tgttttgtat agttacgagt
     8041 cagggtaacg ggacgcacct gattattgct acggcctgct ggacgcatat tgaaatctcc
     8101 ggcttgaaac aaatgtggct gcgcattata cggacttccg gcggttattc ctatcctgac
     8161 aaggcatcga tggctataat ccttccacct ctccttttat aaacaggaac gtctatgatc
     8221 cgcagtatga ccgcctacgc ccggcgtgaa atcaagggtg aatgggggag cgcaacctgg
     8281 gaaatgcgct cggtaaacca gcgttatctg gaaacttact ttcgtctgcc ggagcagttc
     8341 cgtagccttg aacctgtcgt tcgcgagcgt attcgttctc gcctgacgcg cggtaaagtg
     8401 gaatgtaccc tgcgctatga gccagatgtt agcgcacaag gtgagctgat cctcaatgaa
     8461 aaactggcta aacagctggt aactgccgcg aactgggtaa aaatgaagag tgacgaaggg
     8521 gaaatcaacc cggttgatat tctacgctgg ccgggcgtga tggcagccca ggagcaggat
     8581 ctcgacgcga ttgccgctga aattctcgcg gcgttggatg gtacgctgga cgactttatc
     8641 gtcgcgcgcg aaaccgaagg tcaggcactg aaagcattga tcgagcagcg tctggaaggc
     8701 gtcaccgccg aagtggtcaa agtccgcgcc catatgccgg aaattctgca atggcagcgt
     8761 gagcgtctgg tcgcgaagct ggaagatgct caggttcagt tggaaaacaa ccgtctggag
     8821 caggagttgg ttctgctggc acaacgaatt gacgttgccg aagagctgga ccgcctcgaa
     8881 gcgcatgtta aagagacgta caacattctg aagaaaaaag aagcggttgg tcgtcgtctg
     8941 gactttatga tgcaggagtt caaccgtgag tcgaacactc ttgcgtcgaa atctatcaat
     9001 gccgaagtga caaactccgc catcgagctg aaagtgttga ttgagcagat gcgcgagcag
     9061 attcagaata ttgagtaact cgtaacacca attctgactt ttcagccttc acaaatgccg
     9121 cccactcaaa cagagcggca tttttcttcc ccgcaacttc aattctgttt tgcgtgcctg
     9181 ctccagattt tgcgatgttt ttttgcccag cacactgaga acgtgagata ctcacaactg
     9241 tatataaata cagttacaga tttactttct ttgcaatcga tatcacatgg agtgggcaat
     9301 gaacgaacat catcaacctt ttgaagagat aaaactgatt aatgcaaacg gagcagaaca
     9361 atggtcagca agacaacttg ggaaactact gggttattca gagtatcgtc actttatacc
     9421 tgtattaacg cgcgccaaag aggcctgtga gaacagtggt cacacaattg atgaccattt
     9481 cgaggagatc ctcgatatgg tcaaaattgg ctcaaatgcc aaaagagcat taaaagacat
     9541 cgtactctcc cgctatgcct gttacctggt agtacaaaac ggcgaccccg cgaaaccggt
     9601 cattgcggca gggcagactt attttactat ccagacccga cggcaggagc ttgctgacga
     9661 tgaagcattt aagcaacttc gtgaagacga aaagcgtctg tttctgagaa atgagttgaa
     9721 agaacataac aaacaattgg ttgaggcagc acagcaagct ggggtagcga cagctactga
     9781 tttcgccata tttcagaatc atggttacca ggggctgtat ggtggattag atcagaaagc
     9841 catccaccaa ctgaaggggc tgaaaaagag tcagaagatc ctggatcata tgggttcaac
     9901 agaactggcg gctaatctct ttcgagctac ccaaacagaa gaaaaactca agcgggatag
     9961 cgtgaattca aaacagcaag ctaataccac acactttgac gtgggtagca aggtgaggca
    10021 aaccattcag gaacttggcg gaaccatacc tgaggagttg ccgaccccgc aggtcagcat
    10081 caagcagttg gaaaacagtg taaaaattac agagaagaaa tagcagaaac gaaaaactct
    10141 ttttgttgat tgagacaccc gatgcgtaag ccaaggtcca ggtgcagtga acacaatggc
    10201 taaacattgc acctttcttt tcccccctca gttttaacct attttttctt atgcattttc
    10261 tcagacaaga agtcagaaga atgcatctac gctacagaaa atagcgattt cacataacta
    10321 aaagttatca atttcctctc accttaaaaa atatcaatcg tgacaatgcg cacaaatcgc
    10381 taccctgcca gacagatttt tagggagaga accatgctgt tacacatttt gtatttggtt
    10441 ggcattactg ccgaagccat gacgggggcg ctggcggccg ggcgacggcg catggataca
    10501 tttggcgtaa ttattattgc aaccgccacc gcaattggcg gagggtcagt gcgcgatatt
    10561 ctgctgggcc actatccgct cggctgggtc aaacacccgg aatatgtgat tatcgtcgct
    10621 accgccgcag tactcaccac tatcgttgct cctgtaatgc cttacctgcg caaagtgttt
    10681 ctggtgctcg atgcgctcgg actggtggtc ttttctatca tcggcgcaca ggttgcactg
    10741 gatatggggc acggcccaat tattgccgtt gtcgcggcgg taaccaccgg tgtatttggc
    10801 ggcgttttgc gcgatatgtt ctgtaaacgc attccgctgg tctttcaaaa agagttgtat
    10861 gccggtgtct catttgcctc cgccgtgctg tacatcgcgc tgcaacacta tgtttctaac
    10921 catgatgtgg tgattatctc caccctggta ttcggctttt ttgctcgttt actggcgcta
    10981 cgtcttaagc tgggattacc ggtcttttac tacagccacg aaggtcacta aggttcaaaa
    11041 cctgtgatct gctgggcagc cagccaactg cccagcttct tgatttgcgc attttccttc
    11101 cattcaataa cctgtctggc gcgcccagat ccagtccccg gcagctgctg ccagaactgc
    11161 tccgtgctaa ataaaagttg cgaccaggac cgttcatcac tggcattaag cgccgcccgg
    11221 gttagcggta ttcccattgc catcacccag cgagtaaaag gctggttacg agccagatta
    11281 aactgatgcc ataactgcgc acttttactt ttcgcgatcc ccggcgtgtt ctgtaattgc
    11341 tctggcgtta ataaaagcca ggaaaagata tgctcaaagc gatgagtctg atgcagcgcg
    11401 cgccaaccgg cctcaccaat gccatccagc cccagaacct gttttgaccc cagccagact
    11461 aagcgtgaaa tgaactgttc ctgacaaaca tcagaagcaa agtagcaggt caacgggtta
    11521 aagcggtttt ctggtggtgt cggttttgta cgttctgcac cgcgccagac cacatcatca
    11581 atgcgaggaa taccctgacc ggcaaggctg acgaggatct gatcaccagg cgcaatatcc
    11641 cactcctgcc agcgcctgac ggaaccaata ttcacccgct ggactttttt atcatccagc
    11701 atgacaggtg cgagtgacgc aaccaccgat attttaccgc tcttacccac cgcaaactga
    11761 atcgccttca cttcggcaac ctgagctaca ggttgatatt tccaggccac cagccactcc
    11821 gcctggcccg gtagccaatg acgggattcg ggttccttag cccctcgcac aaccacgcca
    11881 tcggtgacga agggtaattt cgctttccac cactcattgc gtacgcgcgc aacctcatca
    11941 gcatttttaa ccgcacgggt atacctctgc gttagagtaa aacctgcggt agccagctcc
    12001 tttaaacgat cggtcattaa ctgcggtcca tccggccatg cccagacaaa aacagccagt
    12061 gagtttagcg tgtcgctatt cccctggcgc atcatcaggc cagcaacttt tgcgcgggca
    12121 tttattcccc ccatttgttg ttggatatgc ccctcgcgct ggagaaatat ttccccctga
    12181 agcgtactgt tggctaaagg cccgctaacg gtttgcggca cagcggaaat taagctaact
    12241 ttctgcgtcc agtcctcgcc tttcaggcca ttaccgcgac tgattgcttt gttcagtttc
    12301 ccgtcccgat aaaccagggt taccgcaacg ccatcaactt ttggctgcac ccaaagatcg
    12361 ctacgttctc gcatccacag acttaatgcg tttttatccg ccattttgcg tacacccgta
    12421 tgggcaaccg gatgcataac cgcaccattt aaaggcggca tcatcacgtc gcggggctcg
    12481 ctcacaaagc agcgttgcca ctgcgtaaga cgggcgctta actgatcgta aacaccgtct
    12541 tccacctcac tttttccttc cttccagtag tcatcgtccc actgttttat ttgctgttgc
    12601 aggcgggaaa tttcttcctg tgctctggct ggcgaccagg ccggacagac cgcccacaca
    12661 gatgattgcc agcacaagat acttattaat atcgccatcc atactttcat cttcacctcc
    12721 gctgtagtca ggctggcaga tatacaacgt ggtgaaggca aagccgagtg gcaaaaacgg
    12781 agtctgcgag gacgcttcct gagaatcgtc tttattgcag taaatcacag gcaaatgcgg
    12841 aaacagctac gcaaaacgca acaactttgc gcaaaaagtg tgagcaaggg ctacgtcaca
    12901 tggccgcgcc gtgtataata agctcgtatg taggctttat ttcgctaatc acatacgaaa
    12961 gatactcatg gctcaaggca cgctttatat tgtttctgcc cccagtggcg cgggtaaatc
    13021 cagcctgatt caggctttat taaaaaccca accgttgtat gacacccagg tttctgtttc
    13081 acacaccaca cgccaaccgc gtcctggtga agtccacggt gaacattatt tctttgttaa
    13141 tcatgatgaa tttaaagaaa tgattagcag agatgcgttc ctcgaacacg cagaagtttt
    13201 tggtaattac tatggcactt cgcgtgaggc cattgagcaa gtactggcga ccggtgtcga
    13261 tgtttttctc gatatcgact ggcagggcgc gcagcaaatt cgccagaaga tgccgcacgc
    13321 gcggagtatc tttattttac cgccgtccaa aattgaactg gaccgccgtc tacgcggtcg
    13381 cggtcaggac agcgaagagg tcattgcaaa gcgtatggcg caagctgttg cagaaatgag
    13441 ccattacgcc gaatatgatt atctgattgt gaatgatgac ttcgataccg cgttgaccga
    13501 tttgaagacc attattcgcg ccgaacgtct gcgcatgagc cgccaaaagc agcgtcatga
    13561 cgctttaatc agcaaattgt tggcagactg aacctgattt cagtatcatg cccagtcatt
    13621 tcttcacctg tggagctttt taagtatggc acgcgtaact gttcaggacg ctgtagagaa
    13681 aattggtaac cgttttgacc tggtactggt cgccgcgcgt cgcgctcgtc agatgcaggt
    13741 aggcggaaag gatccgctgg taccggaaga aaacgataaa accactgtaa tcgcgctgcg
    13801 cgaaatcgaa gaaggtctga tcaacaacca gatcctcgac gttcgcgaac gccaggaaca
    13861 gcaagagctg gaagccgctg aattacaagc cgttaccgct attgctgaag gtcgtcgtta
    13921 atcacaaagc gggtcgccct tgtatctgtt tgaaagcctg aatcaactga ttcaaaccta
    13981 cctgccggaa gaccaaatca agcgtctgcg gcaggcgtat ctcgttgcac gtgatgctca
    14041 cgaggggcaa acacgttcaa gcggtgaacc ctatatcacg cacccggtag cggttgcctg
    14101 cattctggcc gagatgaaac tcgactatga aacgctgatg gcggcgctgc tgcatgacgt
    14161 gattgaagat actcccgcca cctaccagga tatggaacag ctttttggta aaagcgtcgc
    14221 cgagctggta gagggggtgt cgaaacttga taaactcaag ttccgcgata agaaagaggc
    14281 gcaggccgaa aactttcgca agatgattat ggcgatggtg caggatatcc gcgtcatcct
    14341 catcaaactt gccgaccgta cccacaacat gcgcacgctg ggctcacttc gcccggacaa
    14401 acgtcgccgc atcgcccgtg aaactctcga aatttacagc ccgctggcgc accgtttagg
    14461 tatccaccac attaaaaccg aactcgaaga gctgggtttt gaggcgctgt atcccaaccg
    14521 ttatcgcgta atcaaagaag tggtgaaagc cgcgcgcggc aaccgtaaag agatgatcca
    14581 aaaaatcctc tcggaaatcg aagggcgttt gcaggaagcg ggaattccct gccgcgtcag
    14641 tggtcgcgag aagcatcttt attcgattta ctgcaaaatg gtgctcaaag agcagcgttt
    14701 tcactcaatc atggacatct acgctttccg cgtgatcgtc aatgattccg acacctgtta
    14761 tcgcgtgctc gggcaaatgc atagcctgta caagccgcgt ccgggtcgcg tgaaagacta
    14821 catcgccatt ccaaaagcga acggctatca gtctttgcac acctcgatga tcggcccgca
    14881 cggtgtgccg gttgaggtcc agatccgtac cgaagatatg gatcagatgg cggagatggg
    14941 tgttgccgcg cactgggctt ataaagagca cggcgaaacc agcaccaccg cgcaaatccg
    15001 cgcccagcgc tggatgcaaa gcctgctgga gctgcaacag agcgccggta gttcgtttga
    15061 atttatcgag agcgttaaat ccgatctctt cccggatgag atttacgttt tcacaccgga
    15121 agggcgaatt gtcgagctgc ctgccggtgc aacgcccgtc gacttcgctt atgcagtgca
    15181 taccgatatc ggtcatgcct gcgtgggcgc acgcgttgac cgccagcctt acccgctgtc
    15241 gcagccgctt accagcggtc aaaccgttga aatcattacc gcaccgggcg ctcgcccgaa
    15301 tgccgcgtgg ctgaactttg tcgttagctc aaaagcgcgc gccaaaattc gtcagttgct
    15361 gaaaaacctc aagcgtgatg attctgtaag cctgggccgt cgtctgctca accatgcttt
    15421 gggtggtagc cgtaagctga atgaaatccc gcaggaaaat attcagcgcg agctggatcg
    15481 catgaagctg gcaacgcttg acgatctgct ggcagaaatc ggacttggta acgcaatgag
    15541 cgtggtggtc gcgaaaaatc tgcaacatgg ggacgcctcc attccaccgg caacccaaag
    15601 ccacggacat ctgcccatta aaggtgccga tggcgtgctg atcacctttg cgaaatgctg
    15661 ccgccctatt cctggcgacc cgattatcgc ccacgtcagc cccggtaaag gtctggtgat
    15721 ccaccatgaa tcctgccgta atatccgtgg ctaccagaaa gagccagaga agtttatggc
    15781 tgtggaatgg gataaagaga cggcgcagga gttcatcacc gaaatcaagg tggagatgtt
    15841 caatcatcag ggcgcgctgg caaacctgac ggcggcaatt aacaccacga cttcgaatat
    15901 tcaaagtttg aatacggaag agaaagatgg tcgcgtctac agcgccttta ttcgtctgac
    15961 cgctcgtgac cgtgtgcatc tggcgaatat catgcgcaaa atccgcgtga tgccagacgt
    16021 gattaaagtc acccgaaacc gaaattaatg ttttatgaac ccaacacgtt atgcacgcat
    16081 ctgcgaaatg ctcgccaggc ggcagcctga tctgaccgtc tgcatggagc aggtccacaa
    16141 acctcataac gtttctgcga ttattcgtac cgcagatgcc gttggcgtac atgaagttca
    16201 cgccgtctgg cctggtagcc gcatgcgcac catggcttcg gcagcggcgg gtagtaacag
    16261 ctgggtacag gtgaaaacac accgcaccat tggcgatgcc gtcgctcatc tcaaaggcca
    16321 gggcatgcag attctggcaa cccatctttc tgataatgct gtcgatttcc gcgaaattga
    16381 ttacactcgc ccgacctgca ttttgatggg acaggagaaa acgggcatca cgcaggaagc
    16441 attggccctg gcggatcagg acatcatcat tccgatgatc ggcatggtgc agtcgctgaa
    16501 tgtttccgtt gcctcagccc tcattcttta cgaagcccag cgtcagcggc aaaatgcagg
    16561 catgtacctg cgtgaaaaca gcatgttgcc ggaagcagag caacaacgcc tgttgtttga
    16621 aggcggctat ccggtgctgg cgaaagtcgc aaaacgcaaa ggcctgcctt atccccacgt
    16681 caatcagcaa ggcgagatcg aagctgatgc cgactggtgg gctactatgc aggctgcagg
    16741 gtaagtgcca tgaaaggtcg cctgttagat gctgtcccac tcagttccct aacgggcgtt
    16801 ggcgcagcac ttagtaacaa actggcgaaa atcaacctgc ataccgtgca ggatctactc
    16861 ttacaccttc ccctgcgcta cgaagatcgc acccatctct accccatcgg agaactactg
    16921 ccgggcgttt atgccacggt ggaaggcgaa gtgctgaact gcaatatctc cttcggcggt
    16981 cggcggatga tgacctgcca gatcagcgac ggttccggca tcctcaccat gcgctttttc
    17041 aatttcagcg cggcaatgaa aaatagcctg gcgacgggcc gccgtgtact ggcttatggc
    17101 gaagcaaagc gcggtaaata tggtgcggag atgatccacc cggaataccg cgtgcagggc
    17161 gatctcagca cgccagaatt acaggaaacg ctcacgccgg tttatccaac aacggaaggc
    17221 gtaaagcagg ccacgctgcg taaattaacc gaccaggcgc tggatctgct cgacacctgc
    17281 gccattgaag aactcctgcc gccggaactg tcacaaggaa tgatgacgct accggaagcg
    17341 ttgcgcactt tgcaccgccc gccaccgacg ctacagctta gcgatctgga aaccgggcag
    17401 catccggcgc aacgtcgtct gattctggaa gaactgctgg cgcacaacct cagcatgtta
    17461 gccttacgtg ccggagcaca gcgttttcat gcccagccgc tgagcgccaa tgacacgctg
    17521 aaaaataaac tcctcgccgc cttaccgttc aagccaacgg gcgcacaggc acgcgtagtg
    17581 gcggagatcg agcatgatat ggcgctggat gtgccgatga tgcgcctggt acagggcgat
    17641 gtaggttccg gtaaaacgct ggtcgccgcc ctcgccgcgc tgcgtgcgat tgcccacggc
    17701 aaacaggtgg cactgatggc accaaccgaa ttactcgccg agcagcacgc caataacttc
    17761 cgcaactggt ttgcaccgct cggtatcgaa gtgggctggc tcgccggtaa gcagaaaggc
    17821 aaagcgcgac tgtctcagca ggaagccatc gccagcggtc aggtgcagat gattgtcggt
    17881 acacacgcca tcttccagga acaggtgcag tttaacggcc tggcgctggt gattatcgac
    17941 gaacagcatc gttttggcgt gcatcagcgt ctggcattgt gggagaaagg ccagcagcag
    18001 ggcttccatc cgcatcagtt gatcatgacc gccacgccga tcccccgcac gctggcaatg
    18061 acggcgtatg ccgatctcga tacctcggtg atagatgagc tgccgccagg ccgcacgcca
    18121 gtgactacgg tcgctattcc tgatacccgc cgtaccgaca tcattgaccg cgtgcgccac
    18181 gcctgcataa ctgaaggtcg tcaggcgtac tgggtttgta cgttgattga agagtcagag
    18241 ttactggaag cgcaggcggc ggaagctacc tgggaagagt tgaaactggc gctaccagaa
    18301 ttgaatgttg gcctggtaca cgggcggatg aaaccagccg agaaacaggc ggtgatggcg
    18361 tcgtttaaac aaggtgagct acatctgctg gttgccacta ccgttattga agtcggtgtt
    18421 gatgtgccta acgccagcct gatgattatc gaaaaccctg agcgtctggg tctggcgcag
    18481 ttacaccagc tgcgcgggcg cgtaggtcgt ggcgcggtgg cttctcactg cgtgctgctc
    18541 tacaaaacgc cgctttctaa aacggcgcaa attcgcctgc aagtgctgcg cgacagtaac
    18601 gacggttttg tgattgcgca aaaagatctg gagattcgcg gccctggcga attgttaggc
    18661 acgcgtcaga cgggtaatgc tgaatttaaa gtggcggatt tactgcgcga tcaggcgatg
    18721 atcccggaag ttcagcgcct ggcgcgacat attcacgaac gttacccaca acaggcaaaa
    18781 gccttgatag aacgctggat gccggagaca gaacgttact cgaatgcgta aaaggcggcg
    18841 gtgagaagac cgccgcttca tcggttaacc ggcaaaaatc ggcaacatta aatacagctt
    18901 aatcaccagc gcattgacga tatcgataaa gaacgcaccg accatcggca ccaccaaaaa
    18961 cgccatgtgc gacgggccaa agcgttcagt gatcgcctgc atgttggcga ttgccgttgg
    19021 cgttgcaccg aggccaaaac cacagtgacc cgcagccagc actgccgcat cgtagttttt
    19081 gcccatcatg cgccaggtaa cgaagatggc atacaacgcc atgaagatgg tctgtaccac
    19141 cagaatcgcc agcatcggca gcgccagcga agccagctcc cacagtttca gccccatcaa
    19201 cgccatcgcc aggaacaacg acaagcttac gttacccagc acggataccg cacgctcaaa
    19261 gacgcggtaa aagcccatta ttgacagacc gttgctcaga atcacgccaa caaacagtac
    19321 acagacgaag gtcggcagtt caaaagcagt gccagccaaa agttgcgcaa caattttccc
    19381 caccgtcagg cagatagcaa tcagcgcgat agtttcaatc agcaccaacg aggtgatcat
    19441 gcgtcccaca tccggctttt caaacgccgt cgggacttcc tggtcatccg gaataccgtt
    19501 cggcgtggtg gagtgtttca ccagatagcg cgccaccgga ccgccaatca agccgcccag
    19561 caccagaccg aacgttgcac aggccatcgc cacttccgtc gcattggtga agccataacg
    19621 ttcaatgaac aatttactcc acgcagcgcc cgtaccgtga ccgccggaaa gcgtaataga
    19681 accggccaac agccccatca gcggatcaag ccctaacagg ctagccatac caatgccaat
    19741 ggcattttgc atcaccaaca gaccaacaac cacaatcaag aagatgccaa ccacacgccc
    19801 accggcacgc aaactggcaa tgttggcgtt caggccaatg gtggcgaaga aagccagcat
    19861 taacggatcg cgcagggaca tatcaaagtt gacttcccag cccatgcttt ttttcagtac
    19921 tagtagcgcc agcgccacca acaaaccacc cgcaacaggt tccggtatgg tgtatttctt
    19981 caaaaaggag acggaatgga ccaacttacg cccgagcagc aacgtcagcg ttgcggcaac
    20041 aagcgttgct aaagtatcga gatgaaacat agttactcct ttgtatccgc atcactcttt
    20101 catactcgtc atacttcatc gcgcagtggc aacttcatga actatgacgg gtatcgagtt
    20161 atgctttcct gggtcaaatt ctcagctgtt aatgaacaaa tggcatggat tttaagcaga
    20221 aaaataccaa aagttatata aaaaagtgca tttatctcat ttaaatattt agatgaggat
    20281 gtggcaaacg tttgcttttc tctttcggtc agctaaaatg cccgctttgc taccacggga
    20341 ttgttttcga tgtctgtttc caccctcgag tcagaaaatg cgcaaccggt tgctcagact
    20401 caaaacagcg aactgattta ccgtcttgaa gatcgtccgc cgcttcctca aaccctgttt
    20461 gccgcctgtc agcatctgct ggcgatgttc gttgcggtga tcactccagc gctattaatc
    20521 tgccaggcgc tgggtttacc ggcacaagac acgcaacaca ttattagtat gtcgctgttt
    20581 gcctccggtg tggcatcgat tattcaaatt aaggcctggg gtccggttgg ctccgggctg
    20641 ttgtctattc agggcaccag cttcaacttt gttgccccgc tgattatggg cggtaccgcg
    20701 ctgaaaaccg gtggtgctga tgttcctacc atgatggcgg ctttgttcgg cacgttgatg
    20761 ctggcaagtt gcaccgagat ggtgatctcc cgcgttctgc atctggcgcg ccgcattatt
    20821 acgccgctgg tttctggcgt tgtggtgatg attatcggcc tgtcgctaat tcaggtgggg
    20881 ctgacctcca ttggcggcgg ttacgcagcc atgagcgata acaccttcgg cgcaccgaaa
    20941 aatctgctgc tggcaggcgt ggttttagcc ttaattatcc tgcttaaccg tcaacgtaac
    21001 ccttacttac gcgtggcctc actggtaatt gcgatggcgg ccggatatgc gctggcgtgg
    21061 tttatgggca tgttgccaga aagcaacgaa ccgatgacgc aagaactgat tatggtgcca
    21121 acgccgctct attacggtct tggcattgaa tggagtctgc tgctgccgct gatgctggtc
    21181 tttatgatca cttcgctgga aaccattggc gatatcacgg cgacctctga cgtttccgaa
    21241 cagccagtgt ccggtccgct gtacatgaaa cgcctgaaag gcggcgtgct ggcaaacggc
    21301 ctgaactcgt ttgtttcggc ggtgtttaac accttcccga actcctgctt cgggcaaaac
    21361 aacggagtga tccagttgac tggtgttgcc agccgctatg tcggttttgt cgtcgcgctg
    21421 atgttgatcg tgctgggtct gttcccggca gtgagcggtt ttgtacaaca cattccagaa
    21481 ccggttctgg gcggcgcaac gcttgtaatg tttggcacca tcgccgcctc cggtgtgcgt
    21541 atcgtttctc gtgagccgct gaaccgtcgg gcgattctga ttatcgcgct gtcgctggcg
    21601 gttggtctgg gcgtgtctca gcagccgctg attttgcagt ttgcccctga atggctgaaa
    21661 aacctgctct cctccgggat cgccgcgggc ggtattactg ccatcgtgct gaatctgatt
    21721 ttcccaccag aaaaacagta attcactggc ggcggtaaga taattaccgc cgcagtctca
    21781 cctctttcac cattcccgcc ttgaggattg cgcgtaaatc gtgcataact cccttatgtg
    21841 catttcacgg gatggaagac catgaaattt attgggaagc tgcttctcta cattctcatc
    21901 gctctgttag tggtgatcgc tggcctctat tttcttctgc aaacccgctg gggagcagaa
    21961 catatcagcg catgggtttc cgagaatagc gactatcatc tggccttcgg ggcgatggat
    22021 caccgttttt ccgcgccatc tcatatcgtg ctggagaatg tcacgtttgg tcgtgatggt
    22081 cagcccgcgc ccctggtggc aaaaagtgtc gacattgcgc taagcagtcg ccaactgacc
    22141 gaaccacgcc atgtcgatac catcctgctg gaaaacggga cgctgaatct caccgaccag
    22201 accgcgccgc taccgttcaa agccgatcgt ctgcaactgc gtgatatggc gtttaatagc
    22261 ccgaatagcg aatggaaact gagcgcgcag cgggtaaatg gcggcgtggt tccgtggtca
    22321 ccagaagccg gtaaagtgct gggtacgaag gcgcagattc agtttagtgc cggatcgctt
    22381 tcgctcaatg atgttcctgc caccaatgta ctgattgaag gcagtattga taacgatcgc
    22441 gttacgctga ctaacctggg tgccgacatc gcccgcggga cattaaccgg aaacgcgcag
    22501 cgtaacgccg acggcagctg gcaagtggaa aatctgcgca tggcggatat ccgtctacaa
    22561 agcgaaaaat cgctaaccga cttctttgcg ccattacgct ctgtcccgtc gttgcagatt
    22621 ggtcgcttgg aagtcatcga tgctcgtctg caaggtccgg actgggcggt gaccgacctc
    22681 gatctcagct tgcgcaacat gaccttcagt aaagatgact ggcagacaca ggaaggcaaa
    22741 ctgtcgatga acgccagcga gttcatttat ggttcgctgc atttatttga cccgattata
    22801 aacgcggaat tttccccgca gggcgtagcg ctgcgccagt tcaccagccg ctgggaaggg
    22861 ggtatggtca gaacgtcagg gaactggctg cgtgacggta aaacgttgat ccttgatgat
    22921 gcggcaattg ccgggctgga atataccttg ccgaaaaact ggcaacagtt gtggatggaa
    22981 acgacacccg gttggttaaa cagcctgcaa ctgaagagat ttagcgccag ccgcaatctg
    23041 atcattgata tcgaccctga cttcccgtgg cagctcaccg cgctcgatgg ttacggtgcc
    23101 aacctgacgc tggttaccga tcataaatgg ggcgtctgga gtggctcggc gaatctgaat
    23161 gccgccgccg cgacattcaa tcgtgttgat gttcgtcgcc cgtcgctggc gctgaccgcc
    23221 aacagcagca cggtgaatat cagcgaactg agtgcattta ctgaaaaagg cattctggaa
    23281 gccaccgcaa gtgtttcaca aacgccacaa cgtcagaccc atatcagcct gaatggacgc
    23341 ggtgtgccgg tgaatatttt gcaacaatgg ggatggcctg aattaccgtt gactggcgac
    23401 ggcaatattc agcttaccgc cagtggagat attcaggcca atgccccgct gaaacctacg
    23461 gttagcgggc aattgcatgc cgtgaacgcc gcaaagcagc aagtgactca aaccatgaat
    23521 gcgggcgtcg tttccagcgg tgaagttaca tcgacggagc cggtgcggta aacatcaatc
    23581 tcgccgggta cgactcagac gtacccggca ttccgtcaat agattacaac gtaattgtca
    23641 gcgcattccc ttgaggcgtc accaccagcc cctgctcact ttcagcctgc gaaccgcctt
    23701 gcagaccatt tactttcaca acattgcgca agcacagcgt ccagttcttc gcctcgcccg
    23761 caccagtcac agtaatcgtg ttgccagtac gtgctgcttt taaagtaaag atcaccgatc
    23821 cgtcagcagc aggcacttca cagacggctt catgcccgtc ttgcagatta aagaggtgga
    23881 atgccgtgcc ttcgtgccac acgtaatcgg gacgttgatc gttgttgccc agcgccagta
    23941 gagtgttatc acgcacataa acgggcagac tcaggaagcc gtgctgctgt ttatgccagc
    24001 gactaccgtc gagttcatcg ttgtgccaca ggtgtgtcca gcgaccttcc ggcaggtaga
    24061 actgcacatc gcccgcttca gtgaacaccg gcgcaaccat cacgttgtcg cctaacatgt
    24121 attgacggtc aaggtaatca caagccggat cgtccgggaa ctccatcatc atggcccgca
    24181 tcatcggcgt accccgcgcg ttcgcacgcg cagcttcacg atacagatac ggcatcatgc
    24241 ggcatttcag ttgcgtgaag aagcgcacca catcacagga ctcatcatcg taggcccacg
    24301 gcacacgata agatttgcta ccgtgtaaac ggctatggct ggagagcaaa ccaaacgcgc
    24361 accagcgttt gtaaacgtgc gccggagcgg tattttcaaa gccgccgata tcgtggctcc
    24421 agaagccaaa acctgaaagg ccaatagaca aaccaccgcg caggctttcc gccattgatt
    24481 cgtagttagc gtaacaatcg ccaccccagt gtaccgggaa tttctgcgca ccgacggagg
    24541 ccgagcgggc aaacaagaca gcttcttcct caccaacggt gtccttgagc acgttccaca
    24601 ccagttcgtt gtagatgtac gcataatggt tatgcatttt ctgcggatcg gaaccgtcaa
    24661 accactgaac atcaattggg atacgttcgc caaagtcggt cttaaagcaa tcaacgccca
    24721 tcgcgaccag acctttcagt ttgtcggcgt accatttgca ggcatccgga ttggtaaagt
    24781 cataaatcgc cagacctggc tgccatttat cccactgcca tagcgaaccg tccgggcgtt
    24841 tgagtaaata gcctttctct tgtaactctt taaagacggg ggatttttga ccgatatagg
    24901 ggttaatcca gacgcagatt ttcagccctt tcgctttcag gcggcggatc atcccttccg
    24961 ggtcagggaa agtcagcggg tcccactcaa aatcgcacca ctggaaggct ttcatccaga
    25021 aacagtcaaa gtggaaaaca tgcagcggca gattgcgttc cgccatacca tcgataaagc
    25081 tgtttaccgt cgcttcgtcg tagttggtgg taaatgaagt ggttagccac aggccgaagg
    25141 accacggggg cggcagcgcc ggacgaccag taaagcgggt ataacgatca agtaccgctt
    25201 tcggcgtcgg gccgtcgata acaaagtatt cgagatattc actctcaacg ctgaactgca
    25261 ctttggagac tttctccgat cccacttcaa aagagacaca ctggggatga ttgaccagta
    25321 ccccataacc acggttagtc atgtagaacg ggatattttt atacgcctgt tcagtacttg
    25381 tgccgccgtc ccggttccag gtctctaccg tctggccatt gcgcaccagg gcagtaaagc
    25441 gctctcccag accgtaaact gtttcgccaa cgccaagatc aagccgctca aacatataat
    25501 tgcgttgatt attcgtgtcc tgcacgtagc cattattttt cacctgacta ccggtaatac
    25561 gttcgccgtt gcgcagaaaa tccagtgacc agaactcacc tttgctgaca cgcgcgctta
    25621 agttgccact tttaaactca gcataacgtt ctgtgttttc gattgtgacc ttcacgtcct
    25681 gcaaaatatt gagcggataa tgaggaccgt tattcagcgc cccctgaaaa tgctcaatcc
    25741 gcacaccgac aataccttcc tgtggggaga aaaagcgcag cgtaaataaa ggcgtatcaa
    25801 gctgccaggt acgttcacgc acatcacggg gggcagcata gaccaccatt tcattatcct
    25861 gctgttcaac ctcgaatacc tgaagcgggt gaatcaaatt gaggccaggt tgaatcaacc
    25921 agtttccatc gctaattttc attgccgttc cttagttctg cacttcttga gaggtgaatt
    25981 gttgctggca acggcgttta ccctgagcca gctgttccat aacggttttc agattgtgcg
    26041 tcgtgagtga gtagtagcgt ttagcgataa tcgcgctcag caaataacag atcgccggaa
    26101 caatcgtgaa tagcgcaata atgatgctaa tcgtggcgct gttctgcgct ttttctgccg
    26161 catcatatcc gccataagcc agcatccagc cgataagagc gccgccgaag gccaacccca
    26221 gtttgagcac aaacagcgtg ccagcaaaac tgatcccggt caggcgttta ccattgcacc
    26281 actcgccgta gtcgacggta tcgaacatca ttacccactg gataggtgtc accagttgat
    26341 gcaacacacc aatcacgaag atgaagacaa acatagtgat gctggcctgc atgggaacaa
    26401 agaacatcgc gaggctaatc actgccagca gggcgttcgt ccaccagaag atagtgactt
    26461 tacatttcca gtcggtcaga ggttttgcca gtgcggaacc aatcaggtta cccacgcaat
    26521 aagtggtgag aaaagcgaca aacacttccg gcgtgcccaa aatccatgtg acgtaataca
    26581 tcatcgcccc accgcgtacg cacaccgcca ggatattgaa aatggttagt aaaccgacaa
    26641 tccgccactg gtcgttttgc cagatatcac gtaaatcttc ccgcatagac gttgttgtag
    26701 atggtgcttc aacgcgttct ttagtggtga agaaacaaaa tgccagcatc atgaatgcca
    26761 ccacggaaag gaccacgata ccgccctgga aaccgagtgg tttattatca ccgccaatta
    26821 aattaaccag tggcatcatc agaacagtag aaagcatgcc tcccgcggtc gccagcacaa
    26881 aacgccagga ttgcagcgag atacgctgag tcgggtcatt ggtgattaca ccacccaatg
    26941 cgcagtaagg gatattgacg acggtataaa gtaaggtaag ttagggtgta agtaattgct
    27001 gcatagatca ttttgccgtt catactgaga tctggcgtgc tataggccag tacacagacg
    27061 atcccgaatg gcagtgcgcc aaacagtacc cacggacgaa atttacccca gcgagagcgc
    27121 gttcgatcgg ccaacaaccc catgcaagga tcggaaatcg catccagtgc acgagcgacc
    27181 aaaaacatgg ttccgacaaa tccggcagga atgccaaaaa tatcggtata aaagaacatc
    27241 atatataaca ttacgttatc gaaaataatg tggctggcgg cgtctcccat gccataacca
    27301 attttctctt taacggacaa cacttcactc ctcatctgct tttccttgat gctatagggg
    27361 tgctgagacc gataacgcat tttgtaaacc atccctgtga gagcgaatat tccgatatct
    27421 ggttattaat ttatgattct tgttttatgt gatcgtggta gcgttaattc cgctcatata
    27481 tcattgtaaa atatgggttt tatataaatt ataatgcttt cgtgataata cgctgcgttt
    27541 attaggcgga aaaaactgat ctgggggatg tagaaactca aggaagtagc tataatgcgc
    27601 cccgcctcca tgtagcaatc gaggcgcgga agatcgtcgt ctccggtgag gcggctggac
    27661 ttcaaatcca gttggggccg ccagcggtcc cgggcaggtt cgactcctgt gatcttccgc
    27721 cagtttacct cttctgaact ctcccaatgt caataaatct cttctaatcc cttgtatatc
    27781 agtatttatt tgtatcctga ggtcaaccta cttcaccccg tatcaagttt cttttggggg
    27841 tactttaggg ggtgttattt cctattgaaa tacaagcacc cccataatag gaaaaatgtg
    27901 atacataaca acggctttta acaatatctt tgttatacaa ggggtgtatt gggggtatca
    27961 ggttagttac tggcagaaat ccccctcagg aacatgaatt catcaaaaag ccggaggatg
    28021 tcatggcatt aaccgatatc aaagtgaaaa ccgcaaaacc caaagacaaa ccctataagc
    28081 ttgctgatgg cggcggtatg tatctactga ttaacactaa tggttctaaa tactggcgca
    28141 tgaagtaccg ctttgctggt aaagaaaaga tgctttctat tggcgtatac cccgatgtaa
    28201 cgctagccga tgctcgcgag aaacgcagtg aagccaggaa actcctcgct gctggaggcg
    28261 atccaaggga ggcaaaaaag gaagaaaaaa tcgcgcagca aatgagtctg aaaaatacgt
    28321 tcgaggctat tgctcgcgaa tggcaccagt taaaagctga tcggtggtca ttacgctatc
    28381 gtgatgaaat cattgatacc tttgaaaaag acatttttcc ctacattggc aagcgcccta
    28441 ttgccgaaat caaaccaatg aagttactgg aagcactgcg taaaatggaa aaacgtggag
    28501 ctctggagaa aatgcgcaag gtccgccagc gctgtggtga ggtgttccgc tatgcaattg
    28561 ttactggccg ggcagactat aacccagccc ccgatcttgc cagtgcttta gctacaccga
    28621 aaaaagtaca ttttcccttt cttactgcta atgaacttcc ccacttcctc aacgatctgg
    28681 cgggttatac cggaagtata atcactaaga cagctactca gatcattatg ttaaccggcg
    28741 tacgaacaca agaactgcgt tttgcacgct gggaagatat cgattttgag acaaaattgt
    28801 gggagatccc ggcagaagta atgaagatga agcgacctca tattgtgccg ctctctgagc
    28861 aggtcattat gctatttaag cagcttgaac caatctcaaa acatcatccc ctagtcttta
    28921 tcggtagaaa tgatccccgc aaaccaatta gtaaggaaag cattaaccaa gtaattgaat
    28981 tacttggata taagggaaga ctcacagggc acggtttcag acacaccatg agtacaattc
    29041 tgcatgaaca aggctttaat tccgcctgga ttgaaatgca gttagctcat gtggataaaa
    29101 attccatcag gggtacctat aatcatgccc tgtatctcga tggccgccgt gaaatgatgc
    29161 aatggtacgc tgattacatt gattcactct ccagccggga aagttaagtc agaaatactt
    29221 ttccctgatg ttttcctcaa ttaagtattt tatcctctgg cctacaaggc tggaggattt
    29281 ttgttattat attatttttt tgcgtcttca aacttattac aaaaacataa ttaaaaacaa
    29341 attgacattt ttaaattggc aattacaaaa tacagccttg cagttatttc gataccttta
    29401 gcaaaggaat agttgattca gatataattt agcatttcaa tcatctgaca tcattataaa
    29461 aaatgagatt cacataaaga taaaattatt gttctcaagg aaagcatgaa ggggcttcgt
    29521 aagcaatatt cattcggtgc caagacagga cttgatacac agaatctcaa cttttatgct
    29581 ctcagactat atcaccacat gttttacagg ctcagaaaat gaacgataga ttatgctttg
    29641 aagtgcacga taacaagggt tatttcgtat ttccggatac ctggtttggc ccattgctgg
    29701 gggagtttga agaagtgctt gatgcatacg acgctgatga aatcagcgag acgagttaca
    29761 tcaacaaact ccgacgcctt gctcaacggg agccggactt tattgatata cacgctcatc
    29821 tggcatatgc atttctggaa cagaatgccc cgcgtaaggc actgaacgcc gctcttaaag
    29881 gactggcggc aggtaatcgt atcatcccgg aaagtttctg tggtgaaata atctggatgc
    29941 atcccgaaaa ccggccttat ctgcgcgcgc tttatgcagc aattctcgca aatgtgcact
    30001 tacagcgcca tcaggatgcg gttatgctca ctgataaaat actggcatac aatcctgaaa
    30061 ataatcaggg ggctcgctgg ttgttgggtt cagagctgtt acgcaccggc gatcatgagc
    30121 gagccttcag tgtactgaaa gagcatgctg atgaattttc accttactgg tatgaactcg
    30181 gtctcctgca ttttctgaac ggggaacatg ttaaggccgc cacagctttt cgccatggct
    30241 ttgccacaaa cacttacatc gcagaaatgc tgtgcggaaa tctgcatcca tttccacttg
    30301 ctgtgtggca tgacttctcc ggctctctgg atacggcaga agattactat gcaacatact
    30361 caccattatg gggacaatat ccggaagcat tgctgtttgt aaactggctc tataaccatt
    30421 cctccgtgct gcatgaacga tcagaaatca ttaaatgcgc cgaaatgctg attcaggaag
    30481 atgatttcga aatatgcgaa agtattttaa gacagcagga gcacctgtgg aaaagaattg
    30541 acaagacact ttctgaggaa attgtacaga aatgcagaaa tatgaacggt gaatatatct
    30601 ggccctggat attgcccttt tccgcagcag ggatgaaaca ttcaagcatc cagtatcagt
    30661 agatattgca ttagtgtaac tttaaaacaa gtaataattt attatccaat aataacactg
    30721 tgttccttcc ccaggtgatt accctggggt tattttctga aagtttcctt tgcaaaagga
    30781 tagcgttgta aattacttta acgaattact ccatgaactc attaaagttc tttcgtaata
    30841 ctttatcgtc aaacacgtta tagctggcat caaaaaccac agatttaccc tgccagcggg
    30901 taatcccctc ctctggcgtt ttcggttgaa ctggtatccc cgtccaacac aggcagccat
    30961 tataaacagt aaaaaattgt attcgctgtt atgaaatcaa tagtcctgac gtactgttga
    31021 atgtgcttgt gttctgaacc tcgtacaacc agtacatttg atatcgaatt ataaacaaaa
    31081 cattgcatcc aactttttta atatggacaa tagttgtcca tacggtggtt ttatattcga
    31141 taaatcaatg gatttcattg agtcacagga ctggtaatgg atgaaaatgc tttaaggttt
    31201 gcctcatact ggcgcaactc gcttgcagat gctgagtcag gaaagggctg ttttgaacgg
    31261 aaagacgccc aaaatttcac tcactggcat gggatagcgg cgggacgtct tgacgaagcg
    31321 attgtcaata aattttttaa gggagaaaaa gacgatgtcg aaactgtcaa tgtcatctta
    31381 cgcccaaaag tttatttccg gttactgcag catggtaagg accgttccgc aggcacgcct
    31441 gatattgtta caccgatagt gacgccagcc ttgctaagcc gtgagggttt tttatatccg
    31501 acgccagcga cctccattcc cagagacctg cttgaacctt tgccaaaagg agcattttcg
    31561 attggtgaga ttgggcagta tgacaaatac aagacgaccc atactgatcc tacccacgta
    31621 atgtggacac ggccctaagc gagattatgg ttttcaaatt gttccgggct gagaccgcca
    31681 caggcactgt gacgacgcca gcgattgtaa tcacactcga tataattaaa caccgttgcc
    31741 cgcattattt cccggctgct aaagcgttcc ccgtggatac attccacctt cagcgaatga
    31801 aagaagcttt ccacacaggc attgtcataa cagtagcctt tcgcactcat actgccacgc
    31861 aggttgtgtc gcttcagcat tgcctgataa tcccctgaac agtattgacc accacggtcc
    31921 gtatgaacaa tgacgctttc cgggcgtctt ctccgccaca acgccatttg cagtgcatca
    31981 caggccagtt gtgctgtcat tcgcggtgac atcgaccagc caatgacgga gcgtgaccac
    32041 aggtcgatga ctactgcgag atacaaccag ccctcatcgg tacgcaagta cgtgatgtca
    32101 cccgcccact tctggtttcg ggccactggc gctgaagtcc tgctccagca gattctccaa
    32161 tacgggcagg ccatgtgcac ggtagctgac cgggctgaac ttccggctgg ctttcgcccg
    32221 cagcccctga cgacgcaggc tggcggcaat ggttttaata ttgaactccg gcagttcgtc
    32281 agcaaggcgg ggagcaccgt atcgctgctt tgcctcaaag aatgccttat ggacagcggc
    32341 atcgcaggtg agccgaaact gttggcgcag gctcatctgg tgacgacgcc tgagccagac
    32401 ataccagccg ctgcgggcaa ctcgaagtac acgacacatc gctttgatgc tgaactctgc
    32461 ccgatgattt tcgataaaga catacttcat ttcaggcgct tcgcgaagta tgtcgcggcc
    32521 ttttggagga tggacagttc ctcagcctgc tccgccagtt gtcgtttaag gcggacattt
    32581 tcagcggcca gttcgctttc gcgctctgac gaactcattt gttgctgctg tttactgcgc
    32641 caggcataaa gctgagattc atacaggctg agttcacggg ctgcggcggc cacaccgatg
    32701 cgttcagcga gtttcagggc ttcgttacga aattcaggcg tatgttgttt acggggcttc
    32761 ttgctgattg atactggttt tgtcatgagt cacctctggt tgagagttta ctcacttagt
    32821 cctgtgtcca ctattggtgg gtaagatcac cctccattaa aactaaaacg ggcgctttct
    32881 gatatatcat ctgtaagcgc agtaatacca ttagtagcac ctctcggcgg cgcattgatt
    32941 attgaatatt ataactggaa tgttatattt cttgttatga tgctgttcag tattataata
    33001 atgttattat caccaaagat aactcatgca aatgagaaag taatatgtca aaacagtgca
    33061 tcctttgtga ccaaagagtt tatctgcggc actatacttg tctcattaat actcagttct
    33121 ctattttgct acatatcaat atcaccatca attttcatgc atacttatgg cctgagtact
    33181 tttagttact caattatttt ctccttctca gttttatttt tcataatcgg aaatcaatta
    33241 tcaaagattg aaaaattatc caacctatgg tttttattac ccaatagcca attttatcgc
    33301 attacaaata aatggttcaa aaacaggatt tgccgcatct attacaggtt ttactcaaac
    33361 aataagcgct ggtacaatca gtttcctgtc agtaaccgta agcgacaacg gtgttggtcc
    33421 cggaactact ttaggtcttg gttcactaat aacttcatta ctgtcaatag ccgtaatcgc
    33481 cattgcagga aataataaaa agtaagtgta ctctgactgc gtatgtatct acttgaaccg
    33541 ccccggtttt cctggagagt gttttatctg tgaactcagg ctgccagatc atcgtttccg
    33601 atggaagcat aataagcttt ttctgcttct gccggaggag tatggcccag cctttccagc
    33661 aatcgtctat tgttatacca gtccacccac gt
//
LOCUS       DAEEUE010000038        32633 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.038, whole genome shotgun sequence.
ACCESSION   DAEEUE010000038 DAEEUE010000000
VERSION     DAEEUE010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32633
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.038"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..956)
                     /locus_tag="KLK84_001926"
     CDS             complement(<1..956)
                     /locus_tag="KLK84_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBE2009266.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1202..1828
                     /gene="gstB"
                     /locus_tag="KLK84_001927"
     CDS             1202..1828
                     /gene="gstB"
                     /locus_tag="KLK84_001927"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HBE2009267.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="KLK84_001928"
                     /pseudo
     CDS             complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="KLK84_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="KLK84_001929"
     CDS             complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="KLK84_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HBE2009268.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3646..4971
                     /gene="rimO"
                     /locus_tag="KLK84_001930"
     CDS             3646..4971
                     /gene="rimO"
                     /locus_tag="KLK84_001930"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBE2009269.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5018..6346)
                     /locus_tag="KLK84_001931"
     CDS             complement(5018..6346)
                     /locus_tag="KLK84_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBE2009270.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLFGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6354..8702)
                     /locus_tag="KLK84_001932"
     CDS             complement(6354..8702)
                     /locus_tag="KLK84_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBE2009271.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFMINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILSAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(8880..9791)
                     /gene="gsiD"
                     /locus_tag="KLK84_001933"
     CDS             complement(8880..9791)
                     /gene="gsiD"
                     /locus_tag="KLK84_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HBE2009272.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9794..10714)
                     /gene="gsiC"
                     /locus_tag="KLK84_001934"
     CDS             complement(9794..10714)
                     /gene="gsiC"
                     /locus_tag="KLK84_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HBE2009273.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10732..12270)
                     /gene="gsiB"
                     /locus_tag="KLK84_001935"
     CDS             complement(10732..12270)
                     /gene="gsiB"
                     /locus_tag="KLK84_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HBE2009274.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12290..14161)
                     /gene="gsiA"
                     /locus_tag="KLK84_001936"
     CDS             complement(12290..14161)
                     /gene="gsiA"
                     /locus_tag="KLK84_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HBE2009275.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14148..15113)
                     /gene="iaaA"
                     /locus_tag="KLK84_001937"
     CDS             complement(14148..15113)
                     /gene="iaaA"
                     /locus_tag="KLK84_001937"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBE2009276.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15317..16552
                     /gene="moeA"
                     /locus_tag="KLK84_001938"
     CDS             15317..16552
                     /gene="moeA"
                     /locus_tag="KLK84_001938"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBE2009277.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            16552..17301
                     /gene="moeB"
                     /locus_tag="KLK84_001939"
     CDS             16552..17301
                     /gene="moeB"
                     /locus_tag="KLK84_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HBE2009278.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17480..18142)
                     /gene="fsa"
                     /locus_tag="KLK84_001940"
     CDS             complement(17480..18142)
                     /gene="fsa"
                     /locus_tag="KLK84_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBE2009279.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18273..19171
                     /locus_tag="KLK84_001941"
                     /pseudo
     CDS             18273..19171
                     /locus_tag="KLK84_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
     gene            19177..21609
                     /locus_tag="KLK84_001942"
     CDS             19177..21609
                     /locus_tag="KLK84_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBE2009280.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21755..22570
                     /gene="ybiV"
                     /locus_tag="KLK84_001943"
     CDS             21755..22570
                     /gene="ybiV"
                     /locus_tag="KLK84_001943"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HBE2009281.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22722..23987
                     /locus_tag="KLK84_001944"
     CDS             22722..23987
                     /locus_tag="KLK84_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HBE2009282.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24228..25820)
                     /locus_tag="KLK84_001945"
     CDS             complement(24228..25820)
                     /locus_tag="KLK84_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HBE2009283.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            26039..26959
                     /gene="ldtB"
                     /locus_tag="KLK84_001946"
     CDS             26039..26959
                     /gene="ldtB"
                     /locus_tag="KLK84_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBE2009284.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(27017..28135)
                     /locus_tag="KLK84_001947"
     CDS             complement(27017..28135)
                     /locus_tag="KLK84_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HBE2009285.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(28132..28599)
                     /gene="mntR"
                     /locus_tag="KLK84_001948"
     CDS             complement(28132..28599)
                     /gene="mntR"
                     /locus_tag="KLK84_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HBE2009286.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28785..28913
                     /gene="mntS"
                     /locus_tag="KLK84_001949"
     CDS             28785..28913
                     /gene="mntS"
                     /locus_tag="KLK84_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HBE2009287.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29185..30768
                     /gene="opgE"
                     /locus_tag="KLK84_001950"
     CDS             29185..30768
                     /gene="opgE"
                     /locus_tag="KLK84_001950"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HBE2009288.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30816..31331)
                     /gene="ompX"
                     /locus_tag="KLK84_001951"
     CDS             complement(30816..31331)
                     /gene="ompX"
                     /locus_tag="KLK84_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HBE2009289.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31384..31449)
                     /locus_tag="KLK84_001952"
     CDS             complement(31384..31449)
                     /locus_tag="KLK84_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009290.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31684..32571
                     /gene="rhtA"
                     /locus_tag="KLK84_001953"
     CDS             31684..32571
                     /gene="rhtA"
                     /locus_tag="KLK84_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HBE2009291.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         8169 a         8699 c         7882 g         7883 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattactgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt
     1021 aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca
     1081 atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta
     1141 actttacttc accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag
     1201 tatgattacg ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct
     1261 cgaagaactg gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca
     1321 cgatgctgat tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga
     1381 aagtgatctc attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg
     1441 gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga
     1501 ctgggcaaac cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac
     1561 accaccggaa gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct
     1621 gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg
     1681 tgtgggcgat atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg
     1741 gacaccgcgt ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg
     1801 caaagtggtg atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact
     1861 ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg
     1921 ctgccctctg tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat
     1981 cacaatgaca tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat
     2041 ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca
     2101 ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag
     2161 ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt
     2221 tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc
     2281 gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcgc accggattcc
     2341 tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc
     2401 tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa
     2461 agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt
     2521 ggcatctggc ggaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg
     2581 ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag
     2641 atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg
     2701 tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag
     2761 tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc
     2821 agtgcccagg gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc
     2881 gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata
     2941 acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt
     3001 ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct
     3061 caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt
     3121 caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac
     3181 atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat
     3241 ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt
     3301 tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca
     3361 cgtatgcagc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc
     3421 tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc
     3481 tattttttga gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac
     3541 atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta
     3601 gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact
     3661 ccccagccga aaatcggctt tgtttccctt ggctgtccga aaaaccttgt cgattcagag
     3721 cgtattctca ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgcg
     3781 aacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa
     3841 gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca
     3901 aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc
     3961 tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc
     4021 ctgagcctgg tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa
     4081 atttcggaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac
     4141 ctggtgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc
     4201 gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat
     4261 cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag
     4321 ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac
     4381 gacgtcatcc cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag
     4441 cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg
     4501 gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt
     4561 gtcggcttcc ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa
     4621 gcacgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat
     4681 gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag
     4741 ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg
     4801 attatcgacg aagtggacga agaaggcgcg attggtcgca gcatggcaga tgcaccggaa
     4861 atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt
     4921 gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag
     4981 gccgggcaaa atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc
     5041 tgccgttttt cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg
     5101 tagtacattc atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct
     5161 ggaaatgaac gatttctttt agtatcgata gcaactcttc cagcgtgccg ccagtataaa
     5221 tcaccgcaaa ttcgtcacct ccgaagcggt agactttacc tttatctacc gggatctgtg
     5281 atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt
     5341 cgttaatgtt tttgaattta tcgacgtcaa acagacacaa ataaccgcct ttttccggca
     5401 gcgcttttaa atcttcatca aaggattttc tgcccattgc tttggtcagg tcgtccagga
     5461 acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc
     5521 catccagtac gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat
     5581 taatcactct gcgtaccaga tagaggcaac tggcacccaa tatcactgcc accaatgcgg
     5641 caaagaaaat aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacgt
     5701 acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt
     5761 ttttagcatt atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca
     5821 ggttctcgct gtccatcacc agcgattttg acagtaaaga aaaacgctcc agaccaaaca
     5881 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt
     5941 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
     6001 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
     6061 gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa
     6121 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
     6181 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
     6241 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
     6301 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
     6361 tttactaatt ttccactttt atcccaagcg gagaatggca tgggtttctg gtacagatac
     6421 ccctgagcct gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg
     6481 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
     6541 tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga
     6601 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
     6661 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg
     6721 atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg
     6781 atggtggtaa aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata
     6841 tgcagtaaga aatcatcctt cagcgacgca cgttgactaa aatgtctgaa ttcacccatt
     6901 gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta
     6961 atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc
     7021 gcgctcagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg
     7081 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
     7141 agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc
     7201 gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg
     7261 gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc
     7321 gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca
     7381 ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
     7441 ctgacgattt tattgcgttc atcaacacca aacaccgcct ccacggcttc cagaccacac
     7501 agtttgatga tccccacagc cagatactga ttattgttgg actcaataat cgccatattt
     7561 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
     7621 atttcggcat ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg
     7681 ccttcgcgaa ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta
     7741 ttaaccagct cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat
     7801 accaccagca acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg
     7861 ttcaataaat taccgccagg ataataaaaa tagatttcga acaatgcatt atttggcact
     7921 gctgatgtct ttgcaatata ctccgtatcg acagaatcaa taatattata attaccttcg
     7981 ctattataaa taatcagttt ttcacgagcg agttgattct tggcaatgat ctcatgacct
     8041 tgtttcaaaa atagaacttc atcattagcg ctgatttgat caagatactc gttcagatac
     8101 cgccagttta aatcgacatg aattttatct accgctaaca aatcgtgatc tttgccgttg
     8161 atacgtagcg taaaagagat acatttttta cccggacgat caaagcaatt gtatgccgga
     8221 ttccagctgt tgttagagtc atctgaagcc accgaccacg gtcgtttcga tgggttaaaa
     8281 tcggcaaaaa taacccgccc tgactcgtcg acaagcggtc catttacttg ttcctgatga
     8341 taaacaacga ttttatcatt cgccctccgc gaaaagagat aatatttatt atcatcaagt
     8401 accagtccca ccgagtctaa aaatggcatc atttccattt tcagaatcac ggcgcgttga
     8461 atctcctctg tattttttaa taaatacagt gagtccttta atgtcagggc cgaacgttca
     8521 atatctttaa agaaatagcc aacgtttttc tgaataagtt caaagcggct atcggcacgt
     8581 tttaccgcgc gttccgatac cagttgatag ttaatcatga aaaaaagacc aataaatgac
     8641 agtgctgcca ataaaaataa aattatcagc cttatcttta tcttttcgta taaactcagc
     8701 atgttaatac atttaacaaa ataactatct gaacgctgtg ccgctgcaaa aatagtccct
     8761 tgctgcggct ttttaaccaa attacccgat tctacaaaat cagcagaaag ttacaacact
     8821 tgcttagggc aataatttcc tgtgaggtga ttaccctttc aagcaatatt caaacgtaac
     8881 tatcctttaa ttttcggatc cagcgcatcg cgtaaaccat cgcccaacaa attgaacgcc
     8941 agtacggtca gaaaaatagc cagggccgga aaaacagcga catgcggcgc gataaccata
     9001 tccgctcgtg cctcattgag cattgctccc cactctggtg tcggcggctg cgcaccgagg
     9061 ccgagaaatg acaggctggc ggcagagata atcgaggtgc caatgcgcat ggtgaaaaac
     9121 accacgatag aagagacggt cccaggcagg atatgacgca ataaaatggt catatcgctg
     9181 gcaccaatac tgcgtgctga ctcaataaag gtttgctgtt tcaacaccag cgtgttgccg
     9241 cgcaccaggc gggcaaacgc ggggatggaa aaaatggcga ctgcgataat cacgttagca
     9301 atgccgcttc ccaacaccgc aacaacagcg atcgccagta aaatacccgg gaaggcaaac
     9361 agcacatcgc aaatgcgcat gatcagccga tcccaccagc cttcataata tccagccaac
     9421 aagcccagca acgtccctat cgccacaccg ataaacacgg caaacacgcc cgccgccagc
     9481 gagatttgcg caccaaccag gacacggctg aaaatgtcgc gccccagtga atcgacgcca
     9541 aaccagtgct gcaaagaagg tccgttattc agattgtcat aatcaaaata attttcggcg
     9601 tcatagggag cgatccagcg tgcaaaaatg gccaccacaa tcaataaaat aacgaataat
     9661 gcggcggtca tcgccatatg ctgacggcga aatcgtcgcc agaattcatg ccacggtgta
     9721 cgtacctggt caggtttgac cagtggcatg gcgtttaaca ccgcctgacg tcgccagtta
     9781 aatagtcgca tccttacttg tacctgatag ccgggttaat ggcggcgtaa agcacatcca
     9841 ccactaagtt gataagaata aattccagcg agaaaagcag aatttccgcc tgaatcaccg
     9901 ggtaatcgcg catttctaca gagtcaacga gtaagcgtcc aagtccgggc cagttgaaaa
     9961 ctttctccac cacgatagaa ccaccgagca aaaagccaaa ctgtaagccc atcatggtca
    10021 ctaccgggat catcgcgttg cgtagcccgt gtttgaggac aacccaggtt tcgctcaccc
    10081 ctttcgccct cgcggtacgc atataatctt cgcttaaaac gtcgacaaat gacgcacggg
    10141 taaagcgcgc catcacggcg gccactgccg cgccgagcgt cagggagggt aaaatgtagt
    10201 gctgccaact gtctgctccc acagtaggca gccagcccag ttcaacggag aatacctgaa
    10261 ttaaaaacat ccccagcgca aatgccggaa acgagatccc cgacaccgca atggtcatac
    10321 tcaatcgatc cggccaacga ttacgccaga cggcggcgat aattcccgcc gccataccaa
    10381 atatcaccgc ccagaccata ctggttatgg tcagccacag cgttggcata aagcggctgg
    10441 caatctcatc ggcaaccgga cgacgcgaca ccatcgacag gccaaaatcc ccctgcacag
    10501 cattgctgat atagtgccag aactggtgat acagcggctg atccaacccc agctgctgac
    10561 gcaccagttc tataacctgc gcatcagctt cgggcccggc aatcaatcgc gccggatcgc
    10621 cgggcagcat gtggacaaat aaaaacacca gcaccgagac gataaacagc gtcggaatca
    10681 accccagtaa tcgtttgata acgtaattaa gcattccact ccctgcgttg cttattgcaa
    10741 atccgcgtct tcaaagctga agccggtatc tggcatgatc caaaaaccgg tcaggttttt
    10801 actgtgtgcc gacaccagtt tttctaccac cagcgggatc cacggcgatt cttgccagat
    10861 gatatcctgc gccgccttat ataagcgggt cttttccgcc ggatcattcg ttttcagtgc
    10921 ctgagccagg aagtcatcca cctgtttatt gctgtaaaac gcggtattaa acagcgtcgg
    10981 tggccagttt tgcgaggcaa acagcggcga tagtgcccag tcggcttcgc cggttgaagc
    11041 cgaccagcca gtgtagaaca tccgcacgcc actctctttt tgccctttac cttcaacttc
    11101 tgccgcccgc tgtccggcat ccatcgcggt cacctgggct ttaatcccga cctgcgctaa
    11161 ctgctgctgg gtaaattgca gcactttctg cgcggtgctg tggttatgtg acgaccacag
    11221 cgtggtactg aaaccgttgg gatatcccgc ctctttcagt aattcgcgcg ctttcactgg
    11281 atcgtaaggc cacggtttat aactttgcgc gtaggcgata cttggcggta ccacaccagt
    11341 agctggcgtt gcatagcccg caaaggcaac tttcaccagc gccggacggt taatggcgta
    11401 attcagcgcc tcacggacct tcgggttatc gaacggcttt tgcgtcacgt tcatactgat
    11461 ataacgctgc ataattgacg gactggccat caactcgata tttttgtttt tctccagcag
    11521 tgtggcctgc tcgtagggaa tggggaaagc aaactgcgct tcaccggttt gcagcattgc
    11581 cgcgcgggtg ttgttatccg ccaccggacg ccaggttatg ctgtccagtt tgggcaatcc
    11641 tggctgccag taacccgcga attttttcac cttcacaaaa tcggtctgat tccaggtatc
    11701 cagttcatac ggtccggttc ccaccggatg aaaaccaatc tccttgccat atttttccag
    11761 cgctgtcggt gaaatcatcg cggtcgccgg atgggcaaga atattaataa acgctgagaa
    11821 cggctgtttg agggtaatct ttaccgttgt cggatcgatc gcctccgttt tggcaatatt
    11881 cttatacagg ttatagcgtt taagatgatt cgccggatcg ctggcccggt ccagattcgc
    11941 ttttaccgcc actgcgttga aatcggtgcc atcctggaat ttaattcctt cccgcaattt
    12001 cacggtgtaa gtaatgccgt catcggaaac ggtataactc tccgccagca cgtttttcag
    12061 tttcatctct ttatccagac cgaacagccc ctggtaaaac gatttcgcta cggcctgaga
    12121 taacgtgtca ttcgcgtcat acggatcgag cgtggtgaaa ttcgatccta ccgccaccac
    12181 cacatctttg gcagcgaatg cacaagatgc catcaacgct gtcgcaatgc ccagcgccac
    12241 taacccacta cggtgtacag ctcttgccat tttattctcc gcctgaatgt tatctacgca
    12301 tgaatgcgta ttctgattgt ggttgtgcga cgtaatgccc cggcccgacg cattgcaacg
    12361 agacggctgc cacctcttcg ccacgcagat gaatattgct gggaagatcg tccgacagca
    12421 gtacacgctg cggtcgttgt cgggacggtt cagcgaccgg aactgccgcc agtaatttac
    12481 gcgtataagg atgctgcggg ttttcgaaga ccgcgcaccg tgaaccaatt tcaacaattt
    12541 gcccgagata catcaccgcc acacgatgac taatccgctc taccacagcc atatcgtggg
    12601 agataaacag atacgcaatg ccgaaatcac gctggagatc gagcaacaag ttgataatct
    12661 gcccgcgaat agaaacatcc agcgccgaaa cggcttcgtc ggcaatgatc acttttggat
    12721 tcaatgccaa cgcgcgagca atgcagatgc gctggcgctg accgccggaa aactcatgcg
    12781 ggtaacgcca ggcatgttca ggtaacaggc ccacgcgctc cagcaaccac gcaacgcgtg
    12841 cagccgcgtc tttacctggc aataaaccgt gtacacgcag cggttcgata atcgaatcac
    12901 cgatggtctg acgtgggtcc agcgaagcgt aagggtcctg aaaaataaac tgaatatccc
    12961 ggcgcaatgc ctgaagtttg ccaggtgaca aggtatcgat tcgctgaccg ttaaagataa
    13021 tttcgccgcc ctgcgattcg accaggcgca gcaacgcccg cccggtagtg gatttaccgc
    13081 tgccagactc acccaccagc gatagcgttt cgccaggcca gagatcaaaa ctgactttct
    13141 caacggcatg cacttcccgc gttacgcgat tcaacaaacc gctgcgcaaa gggaaacggg
    13201 tgacaagatt acgcacccgt aaaacaggtt cgccatccac caccgttttc tgctcgatgg
    13261 ggggttcctg tttcgctgga tgttcaagcg atatcaatgg gaaacgtcgg ggataatcta
    13321 accctttcat cgcaccaagt tgcggaacag cagctaacag cgcacgggtg taaggatgtt
    13381 gcggtgcatg aaaaatctgt tcgacggtac ccgtttccac cgcctcgccc tgatacatca
    13441 ccagtacccg atcggcaatc tctgccacca cgcccatatc gtgagtgata aagataacgc
    13501 ccatcgacat ctctttttgc aatactttga ttaattgcag gatctgcgcc tgaatagtga
    13561 catccagcgc ggtggttggc tcatcggcaa tcagcaccgc cgggcggcat gacagcgcca
    13621 tcgcaatcat cactcgctgg cgcatcccgc cagagagttg atgcgaataa cgtgaaagaa
    13681 tggtttgtgc ctccggaatg cgtacctgat ccagcatccg cttcgcctcg accatcgctt
    13741 cttcacgact ggcgttctga tgcagacgaa ttgattcggc aatctgttca cccacagtaa
    13801 atactgggtt cagcgatgtc atcggctcct gaaatatcat cgccatatcc gcaccgcgca
    13861 catggcgcat ttgtgcagcg ctctgctcgc taagttcaat cacatcacga ctgcgccgcc
    13921 gcaacagcat tttatcgcac tgtactaaac cgcccgcctg ttccaacaga cgcatcaatg
    13981 ccagcgcagt cactgactta ccggagccgg attcgcctac aattgccagc gtctcaccgc
    14041 gttgcagact aaaagagaga ttgcggaccg cagctatttt ctgctggtcc tgcataaagg
    14101 caatattaag attttcaacc gccagcacat taccggcatc aagttcatca ctgtgtggca
    14161 acggtgtccc ctttttcacg gtagataccg gtggttggcg tatcgcctgc gtagccccag
    14221 gcgcgataca ttccttcggt gttaaacggt agcgcgacat tcccttcatg gtcgatagcg
    14281 attaagccac cgctaccgcc aagcgcaggg agtttttcca ttactacccg ctcgcaggct
    14341 tccgcgagac ttaatccgcc gtaatccatt aacgcggcga tgtcatatgc cgccagcgcg
    14401 cggatgaaga cttcgcctgt gccggtacaa gaaaccgcca cactggcgtt attggcgtag
    14461 catccggcac ccactaaggg actatcgcca actcgtccgg gtaatttatt ggtcattccg
    14521 cctgtggacg tggctgccgc cagattgccg tctaaatcca acgccacggc ccccacggtg
    14581 cccattttct gtttttcatc cagtggcgca ccgctatggt cgaggactgt tgccccttcc
    14641 tcgcgcgctg ccagtagttg ttcataacgc aaaggcgtgg agaaaatctc cggtgagacg
    14701 cgctccatac catgagcaaa cgcaaaattt tctgcccctt cgccaatcat catcacatgc
    14761 gggctttgct ccatcaccag ccgggcggca agaaccggat tacgcagatg actaacgccc
    14821 gccaccgcac cggctttcag ggtgttacca tccatcacac aggcgtccag ttcatgggtt
    14881 tcatcacgcg taaagacagc gccaattccg gcgttaaaca gtggacactc ttccagcaga
    14941 cgcaccgctt ccgtcaccac atccagcgca ctttcgccgg cttccagcat tttctgcccg
    15001 gtttcaacaa tggcagacaa cgcctcgatg tagcgtaatt cctgttgcag actcatctgc
    15061 gcgcggctaa ttgcacctgc gccaccatga attgcaatga ctgctttgcc catagtattc
    15121 gtccattaag ggtggctcgc cacttttgct ataaatatca gaatttttat gaattactca
    15181 ttcaattttt gaatatagaa agatggatat ggcatgtaaa ggcaggagcc aacgctacgc
    15241 ccgaggtggc atacagtcta tggcgttttt ctgacataat aggcaaattc gattttgcct
    15301 ccgcaggagt gttttcatgg aatttaccac cggattgatg tcgctcgaca ccgcgcttaa
    15361 tgagatgctt tctcgcgtta ccccactgac cacccaggaa acgctgccac tggttcagtg
    15421 ttttggtcgt attctggcga gcgatgtcgt ttcgccactg gatgtcccgg ggtttgataa
    15481 ctccgctatg gacggctacg cggtgcgttt agccgatatt gcctccgggc aaccgctgcc
    15541 cgttgccggt aaatcctttg ccggtcagcc ataccatggt gaatggcctg cgggtacctg
    15601 cattcgtatt atgaccggtg cgccggtgcc ggaaggctgc gaagcggtgg tgatgcagga
    15661 gcagactgaa caaacggaca atggcgtgcg ttttactgct gaagtgcgta gcgggcaaaa
    15721 tattcgccgt cgcggtgaag atatctctgc aggtgcggtt gttttcccgg cgggaactcg
    15781 cctgactacc gcagagctgc cagtaattgc ttcactgggg attgccgaag ttccggtgat
    15841 tcgtaaagtg cgtgtagcgc ttttttctac cggtgatgaa ctccagttgc ccggtcagcc
    15901 gctgggcgac ggccaaatct acgacaccaa ccgtctcgcc gtacacctga tgttagaaca
    15961 gctgggttgc gaggtaatca acttagggat tatccgcgac gatccacatg ccctgcgcgc
    16021 cgcatttatt gaagccgaca gccaggcgga tgtagtgatc agttccggcg gtgtttcagt
    16081 gggtgaagcg gattacacca aaatgattct tgaagagctg ggggagatcg ccttctggaa
    16141 gctggcgatt aaaccaggta aaccgttcgc gttcggtaaa ctcagcaata gctggttctg
    16201 cggcctgccg ggcaacccgg tttcagcgac gctgaccttc tatcaactgg tacagccttt
    16261 gctggcaaaa ctaagcggca acaccgccag cggcctgccc gcgcgccagc gcgtacgcac
    16321 agcgtcccgc ctgaaaaaaa cgccaggacg tcttgatttc cagcgcggcg tgctgcaacg
    16381 caacgccgac ggtgaacttg aagtgacgac caccggacat cagggttcac atatatttag
    16441 ctcctttagt ctcggcaact gctttatcgt gctggaacgc gatcgcggca atgtggacgt
    16501 gggcgaatgg gtggaagtag aaccgtttaa cgcgttgttc ggaggcctgt aatggcggaa
    16561 ctcagcgatc aggagatgct gcgctacaac cggcaaatca ttctgcgcgg ttttgatttt
    16621 gacggtcagg aggcgctaaa agattctcgt gtgctggtag tcggcctcgg tggactcggc
    16681 tgcgcagctt cgcagtatct ggcaagcgcc ggtgtcggca acctgacgct gctcgacttc
    16741 gacacggttt cgctctcgaa tctgcaacgc cagacactgc acagtgatgc cacggtcggg
    16801 caaccgaagg tggaatccgc ccgtgacgcc ctgacgcgga tcaacccaca tatcgcgatt
    16861 acgccagtca atgcactgct agatgacgca gaacttgcag cattgattgc tgaacacgat
    16921 ctggtgctcg actgtacgga taacgttgcg gtacgtaatc aactgaacgc aggctgtttt
    16981 gccgcgaagg tgccgctggt ttccggcgcg gcaattcgta tggaagggca aatcacggtc
    17041 ttcacttatc aggacggcga accgtgctat cgctgcctta gccgtttgtt tggcgagaac
    17101 gcgttaacct gcgtagaagc aggcgtgatg gcaccgttga taggcgtaat tggttcgttg
    17161 caggcaatgg aagcgatcaa actgctggca ggttatggca aaccggccag cgggaaaatc
    17221 gtcatgtacg atgcgatgac ctgtcagttt cgcgaaatga aactgatgcg caatccgggg
    17281 tgtgaggtgt gcgggcagta attgccacct ccaagcctga taagacgcga caagcgtcgc
    17341 atcaggcatt tgctccagac gccagatgcg gcgtgaacgc cttatctggc ctacggttcg
    17401 ggcacagatt tccaagcgcg gcaggcatcg gtgcactccg ccacgtcatc agcaatttat
    17461 gaggatgcag aacgtccggt taaatcgacg ttctgccaaa cgctccctgc cagtcctgct
    17521 caaacttcgt cacagcggct tccaccgccg gatagctaat catctgttgt gccacatcca
    17581 gtggcagagt aattgattca catccggcca gtaagcagtc cagcgcctga cgcggggttt
    17641 tgaaactcgc tgccagcact ttcgcctgcg gcgcatgcat tttcaataac tggtgtaagt
    17701 cggtcacagt ctgaatgccg ctaccgccct gagcatcaat acgattaacg taaggcgcaa
    17761 catattccgc acctgccagc gccgacagca gcccttgtgc cgcgccatat accgcggttc
    17821 ccagcgtcgg aatcccttcc gcttttaaca tcttaatcgc tgccagcccc tcggctgtca
    17881 ccggaacttt caccacgata tccgcaataa tagaacgcag cttaagcgcg tcattcacca
    17941 tcccttcagc agtggtagcc attacctggg caaacagacg cccctgaccg cccatcgctt
    18001 catgaagttg cggaagcaca acatccagcg gttttttacc cgcggcgata atgcttgggt
    18061 tagtggtcac acccgccagc ggaaaaatac gtgacagcgc cttcaccgca acaacgtctg
    18121 aagtatccag atacagttcc ataaccatcc tcaaaatgta ctttttgtct gaatgtagac
    18181 tatcacggga aaactctctg aacagttgac gcacatcaat ctgactttca ttcgaaagta
    18241 ttttaatgtt tgaacgaaac agagaggcaa ctatgatttt caatattcag cgctactcga
    18301 cccatgatgg ccccggtatc cgcacggtcg tatttcttaa aggctgttcg ctgggctgcc
    18361 gctggtgtca gaacccggaa agccgcgccc gtacgcagga tctgctgtat gacgcacgac
    18421 tgtgtctgga aggctgcgag ctgtgcgcta aggccgcgcc ggaagtgatt gagcgcgcgc
    18481 tgaatggttt gcttattcat cgggaaaagt taaccccgga gcatctgacg gcgttaaccg
    18541 actgctgtcc gacacaggcg ttaaccgtgt gtggtgaagt gaaaagcgtt gaggagatca
    18601 tggcgaccgt tctgcgcgat aaaccgtttt acgatcgcag cggcggcggc ttaacgcttt
    18661 cgggtggtga acccttcatg cagccggaaa tggcgacggc gctgctgcaa gcctgccacg
    18721 aggcaggcat tcatactgcg gtagaaacct gtctgcatgt gccgtggaaa tatatcgccc
    18781 cttctctgcc ctatatcgat ctgtttcttg ccgatttaaa acacgttgcc gacgcgccgt
    18841 ttaaacagtg gaccgacggt aacgccgcca gggtgctgga taacctgaaa aaactcgccg
    18901 cagcgggcaa aaaaatcatt atccgcgtgc cgctgattca gggctttaat gccgacgaaa
    18961 cctctgtaaa agccattacc gattttgccg ccgacgagct gcacgttggc gaaattcatt
    19021 ttctgcccta ccacacgctg ggcatcaaca aatatcactt acttaatctg ccctatgacg
    19081 cccggaaaaa ccgcttgatg cgccagagct gctcgatttt gcccagcagt atgcctgcca
    19141 gaaagggtta accgcgacct tacgaggata acaaccatga ccacactgaa actggacacg
    19201 ctcagcgacc gcattaaagc gcacaaaaat gcgctggtgc atattgtgaa gccgccagtc
    19261 tgtaccgagc gcgcgcagca ctataccgag atgtatcaac aacatctcga taagccgatc
    19321 ccggtacgtc gcgcgctggc actggcgcat cacctggcga atcgcaccat ctggatcaaa
    19381 cacgatgagt tgatcattgg caaccaggca agcgaagttc gcgccgcgcc gatcttcccg
    19441 gaatatactg tctcgtggat cgaaaaagag attgatgatc tggcagatcg tcccggtgct
    19501 ggctttgcgg tgagcgaaga gaacaaacgt gttctgcatg aagtgtgccc atggtggcgc
    19561 ggtcagacgg tacaggatcg ctgctatggc atgtttaccg atgagcaaaa aggtctgctg
    19621 gcgaccggca tcattaaagc ggaaggcaat atgacctccg gcgatgcgca cctggcggtc
    19681 aatttcccgc tgctgctgga aaaagggctt gatggtctgc gtgagaaagt agcggaacgt
    19741 cgctcgcgca tcaatctgac ggtgctggaa gatttgcacg gcgaacagtt cctgaaagcg
    19801 attgatatcg tgctggtggc agtcagtgaa cacattgaac gtttcgctgc cctggcgcgt
    19861 gcaatggccg cgaccgaaac ccgcgaaagc cgtcgcgatg aactgctggc gatggcagaa
    19921 aactgcgatc ttatcgccca ccagccgccg cagactttct ggcaggcgct gcaactgtgt
    19981 tacttcatcc agttgatttt gcagattgaa tctaacggtc actcggtgtc gtttggtcgt
    20041 atggatcagt atctctaccc gtactatcgc cgcgacgttg aactgaacca gacgctggat
    20101 cgcgaacacg ccatcgaatt gctgcatagc tgctggttga aattgctgga agtgaacaaa
    20161 atccgctccg gctcccactc aaaagcctct gcgggaagtc cgctgtatca gaacgtcacc
    20221 attggcggac aaaatctggt tgatggtcaa ccaatggacg cggtgaatcc actctcttac
    20281 gcgatcctcg aatcctgcgg tcgcctgcgt tccactcagc ctaacctcag cgtgcgttac
    20341 catgcaggaa tgagtaacga tttcctcgac gcctgcgtac atgtgatccg ctgcggcttc
    20401 gggatgccgg cattcaacaa cgacgaaatc gtgatcccgg aatttattaa actcggtatt
    20461 gaaccgcagg acgcttatga ctacgcagcg attggttgta tagaaaccgc cgtcggtggc
    20521 aaatggggct atcgctgtac cggcatgagc tttatcaact tcgcccgcgt gatgctggcg
    20581 gcgctggaag gcgggcgtga tgccaccagc ggcaaagtgt tcctgccaca agaaaaagcg
    20641 ttgtcggcag gtaacttcaa caacttcgat gaagtgatgg acgcgtggga tacgcaaatc
    20701 cgttactaca cgcgtaaatc aatcgaaatc gaatatgtcg tcgacaccat gctggaagag
    20761 aacgtgcacg atattctctg ctcggcgctg gtggatgact gtattgagcg agcgaaaagt
    20821 atcaagcaag gcggcgcgaa atatgactgg gtttctggcc tgcaggtcgg tattgccaac
    20881 ctcggcaaca gcctggcggc agtgaagaaa ctggtgtttg aacagggcgc gattggtcag
    20941 caacagctgg ctgccgcact ggcggatgac ttcgatggcc tgactcatga gcaactgcgt
    21001 cagcggctga ttaacggtgc gccgaagtac ggcaacgacg atgataccgt cgatacgctg
    21061 ctggctcgcg cttatcagac ctatatcgac gaactgaaac agtaccataa cccacgctac
    21121 ggtcgtggtc cggttggcgg caactattac gcgggtacgt cgtctatctc cgctaacgta
    21181 ccgtttggcg cgcaaactat ggcaacgccg gacgggcgta aagcccacac cccgctggca
    21241 gaaggcgcta gtccggcctc tggtactgac catctcggcc ctactgcggt cattggctca
    21301 gtgggtaaac tgcctacggc agcgattctc ggcggcgtgt tgctcaacca gaaactgaat
    21361 ccggcaacgc tggagaacga atctgacaag cagaaactga tgatcctgct gcgtaccttc
    21421 tttgaagtgc ataaaggctg gcatattcag tacaacatcg tttcccgcga aacgctgctg
    21481 gaggcgaaaa aacatccgga tcagtaccgc gatctggtag tgcgtgtcgc gggttattcc
    21541 gccttcttca ccgcgctctc tccagacgct caggacgata tcatcgcccg tactgaacat
    21601 atgctgtaat ctcttcggcc cggctgcggc gtcgccgggc taaatcattc acatcatcaa
    21661 tttcatcctt actttcattc gaaatataat ttgtgctctg cgtcacattg ttattacact
    21721 gttacggttt cattttcgag ccaggagcac acctatgagc gtaaaagtta tcgtcacaga
    21781 tatggacggt acttttctta acgacgccaa aacgtacaac caaccacgtt ttatggcgca
    21841 atatcaggaa ctgaaaaagc gcggcattaa gttcgttgtc gccagcggta atcagtatta
    21901 ccagcttatt tcgttctttc ctgagctaaa ggatgagatc tcttttgtcg cggaaaacgg
    21961 cgcactggtt tacgaacatg gcaagcaact gttccacggc gaactgaccc gacatgaatc
    22021 gcggattgtt attggcgagt tgctaaaaga taagcaactc aattttgtcg cctgcggtct
    22081 gcaaagtgca tatgtcagcg aaaatgcccc cgaagcattt gtcgcactga tggcaaaaca
    22141 ctaccatcgc ctgaaacctg taaaagatta tcaggagatt gacgacgtac tgttcaagtt
    22201 ttcgctcaac ctgccggatg aacaaatccc gttagtgatc gacaaactac acatagcgct
    22261 ggatggcatt atgaaacccg tcaccagtgg ttttggcttt atcgacctga ttattcccgg
    22321 tctacataaa gcaaacggta tttcgcggtt actgaaacgc tgggatctgt caccgcaaaa
    22381 tgtggtagcg attggcgaca gcggtaacga tgcggagatg ctgaaaatgg cgcgttattc
    22441 ctttgcgatg ggcaatgctg cggaaaacat taaacaaatc gcccgttacg ctaccgatga
    22501 taataatcat gaaggcgcgc tgaatgtgat tcaggctgtg ctggataaca catccccttt
    22561 taacagctga cctctctacc ggagccatgc gctccggttt tcccttcttt ttacatcctg
    22621 tacatcacat caaatcatta aacttgcatt aacttattta taacttgtat tgtcacatgc
    22681 aaattagcat agtttcgaat gaaagttaat gcgaggtggt tatggcttct acttttacca
    22741 gcgacacatt gcctgccgat cacaaagcag ctatccgtca gatgaagcac gcgctgcggg
    22801 cgcagcttgg cgatgtccag cagatcttta atcagctaag cgatgacatt gccacgcgag
    22861 tggctgaaat caacgcactc aaagcacagg gcgatgccgt ctggccggtg ctgtcttatg
    22921 ccgatatcaa agcaggtcat gttactgcag agcagcgcga acagattaaa cgtcgcggtt
    22981 gtgcggtgat aaaaggccat ttcccccgcg aacaagcgct aggctgggat cagtcgatgc
    23041 tggactatct gaaccgcaac cgctttgacg aggtctacaa aggccccggc gataatttct
    23101 tcgggacgct cagcgcttca cgtcccgaga tttaccccat ctactggtcg caggcgcaaa
    23161 tgcaggcccg ccagagtgaa gaaatggcga atgcgcagtc gtttctcaat cgtctgtgga
    23221 catttgaaag tgatggaaag caatggttta acccggatgt gagcgtcatc taccctgacc
    23281 gtatccgccg ccttccgccc ggaacgacct ccaaaggtct tggagcgcat accgactccg
    23341 gggcgctgga acgctggctg cttccagcgt atcagcacgt tttcgccaac gtctttaatg
    23401 gcaatctggc gaaatacgat ccctggcatg cggcacatcg tacggaagtt gaagagtaca
    23461 cggtggacaa caccaccaaa tgttccgtgt ttcggacatt ccagggctgg actgcgctct
    23521 cggatatgct gcctggtcag gggttgctgc acgtcgtgcc cattcctgaa gccatggcgt
    23581 acgtactgtt acgtccgctg cttgatgatg tgccggagga tgaactgtgc ggcgtagcgc
    23641 ccggaagagt gttgccggta tcagagcaat ggcatccact gttaattgag gcgttaacca
    23701 gcattccaaa actcgaggcc ggagactccg tctggtggca ctgcgacgtc atccattccg
    23761 ttgcccccgt tgaaaatcaa cagggttggg gcaacgtgat gtacattcct gcggcaccga
    23821 tgtgcgagaa aaatcttgcc tacgcgcaca aggtgaaggc cgcactggaa aaaggcgcat
    23881 cgccgggcga cttcccgcgc gaggactatg aaacaaactg ggaaggacgc tttacgcttg
    23941 ccgacctcaa cattcacggt aagcgagcgt tgggcatgga tgtttgagag ttaatggaag
    24001 tgaattgatg tgggattaag aaaatcgatt cgacgttact cagattgctg acaagtgcgc
    24061 tttcttaatg cctgatgcgg cgttaacgcc ttatccggcc tacaaagtcc tgcagattca
    24121 atatattaca ggtactgcgt aggcctgata aacgtagcgc atcaggctat tttgcatttg
    24181 tcatcagccg tcgtcacccc gacagtatcg gggtgacgga taatcactta ctcgatccct
    24241 ttactacgca ggtaatcttc gtaattaccg ctaaagtcga tcacgcgttc cggggtgatt
    24301 tccagaatgc gggtcgccag ggagcttacg aactcacggt cgtgtgaaac aaagatcagc
    24361 gtgccctgat acagttccag cgccatgttc agtgactcga tggattccat atccaggtgg
    24421 ttggtcggtt cgtccatgat cagaatgttc ggcttctgca tcattaactt accaaacagc
    24481 atccgccctt tttccccacc ggaaagcact ttagctggct ttttgatgtc gtcctggctg
    24541 aacagcaaac gaccgagaat gctgcgtacc gcctgctcgt catcgccttc ctgcttccac
    24601 tgactcatcc attcgaacac ggtcagatca ttttcaaact catattcgtg gtcctgggcg
    24661 tagtaaccaa tgcgcgcgtt ttcagaccat tttacggtgc cgctgtccgg ttgcagatcg
    24721 cccaccagcg ttttcagcag cgttgattta ccgacgccgt tggtacccag taccgcaagt
    24781 ttttcaccga cctccagcag caggttgaga tttttaaaca gtggaccgtt atcaaaccct
    24841 ttggtcagac cttccacttc cagcgcgtta cggaacagtt tcttatcctg ttcaaaacgg
    24901 ataaacgggt tctgacggct ggaggctttc acctcttcca gtttgatttt atcaatctgg
    24961 cgcgcgcgcg aagttgcctg gcgagatttc gaggcgttgg cgctaaagcg gctaacgaaa
    25021 gattgcaact cagcaatctg cgctttcttc ttggcgttat cggccagcag acgttcacgc
    25081 gcctgggtcg ccgccgtcat gtactcatcg tagttacccg gataaacgcg cagctcgccg
    25141 taatccagat ccgccatgtg ggtacagacc atgttaagga agtgacggtc gtgcgagatg
    25201 atgatcatgg tgctgtcacg ctcgttcagc acctgttcca gccagcgaat ggtgtcgatg
    25261 tcgaggttgt tggtcggttc gtcgagcagg agaatatccg gctcagcaaa cagcgcctgc
    25321 gccagaagca cacgcagctt ccagccagga gcaacttcac tcatcgggcc gtagtgctgt
    25381 tccactggaa ttcccacgcc aagcaacagt tcaccggcgc gagcttccgc agagtaaccg
    25441 tccatttcgc cgtatttcac ttccagatcg gccactttat agccgtcttc ttcactcatt
    25501 tccggcaaag catagatgcg gtcgcgctcc tgcttcactt cccacaactc tttatgcccc
    25561 atgatcaccg tatccagcac agtgaactct tcaaaggcaa actgatcctg acgcagctta
    25621 ccaatgcgct cgttgggatc gagggaaacg ttacccagcg tcggctcaag gtcgccgccg
    25681 agaatcttca taaaggtgga tttaccacta ccgttcgcgc caatcaggcc gtaacggttg
    25741 ccgccgccga atttgacgga aatgttttca aacaacggct tactgccgaa ctgcatggtg
    25801 acgttactgg aaactaacac aggcgtatcc tgaaaagaga tatgacaaac cgcgcattat
    25861 gccataagtc tgaaagaaga tcctgctcct cgcttaaggt ctacactcaa gccaaaggca
    25921 aaaaatgtga tttcgtacac atctgatttc actgtgagct ggaatgaact tataatgcgc
    25981 ttccaatact ctaatattct caacccaatg gcctgccagg cacaaaatct cgcttaacat
    26041 gaatatgaaa ttgaaaacat tattcgcagc ggccttcgct gttgtcggct tttgcagtac
    26101 cgcctctgcg gtaacttatc ctctgccaac cgacgggagt cgcctggttg gtcagaatca
    26161 ggtgatcacc attcctgaag gtaacactca gccgctggag tattttgccg cggagtacca
    26221 gatggggctt tccaatatga tggaagcgaa cccgggtgtg gataccttcc tgccgaaagg
    26281 cggtactgta ctgaacattc cgcagcagct gatcctgccg gataccgttc atgaaggcat
    26341 cgtcattaac agtgcagaga tgcgtctgta ttactatccg aaagggacca acaccgttat
    26401 cgtgctgccg atcggcattg gtcagttagg caaagatacg cctatcaact ggaccaccaa
    26461 agttgagcat aagaaagcag gcccgacctg gacgccgacc gccaaaatgc acgcagagta
    26521 ccgcgctgcg ggcgaaccgc taccggctgt cgttccggca ggtccggata acccgatggg
    26581 tctgtatgca ctctacatcg gtcgcctgta tgctatccat ggcaccaacg ccaacttcgg
    26641 tatcggcctg cgtgtaagtc atggttgtgt gcgtctgcgt aacgaagaca tcaaattcct
    26701 gttcgagaaa gtaccggtcg gtacccgcgt acagtttatt gatgagccgg taaaagcgac
    26761 caccgagcca gacggcagcc gttatattga agtccataac ccgctgtcta ccaccgaagc
    26821 ccagtttgaa ggtcaggaaa tcgtgccaat taccctgacg aagagcgtgc agacagtgac
    26881 cggtcagcca gatgttgacc aggttgttct tgatgaagcg atcaaaaatc gttccgggat
    26941 gccggttcgc ctgaattaat cgtcaaaacg taaaacaaag gcggactcat aatccgcctt
    27001 ttttatttgc cagaccttac caggccggga tcataactaa caaaacgtat cccaccagcg
    27061 ccgcccacaa cagcatcggt attgaataga gatggaaacg ccaccagatg cggcgatcgt
    27121 tcgccatacg tagcgcaatc aaatttgcca gcgatcctgg taataaacca aagcccccca
    27181 cgtttaccgc ccatgccagt aataaagacg gaggtacata attgagcagc aaaatggtac
    27241 tcggcacgtt actgatcacc tgcgataaac cgactgccgt taaccataac ccgggttcag
    27301 ataaatgact cacgttaccc aacacgcctt gcaacgctgg aagctgggtc agtaaatgga
    27361 cgtcgataaa catcgccata aacaccagca gcaacgtcca gtccacactc aacaccacgc
    27421 gacgcgccag cagcacaaag cccgccgcca caatcaccag tccccacagc tcttgtttga
    27481 actccagcgc cgtcagaaag acgatataca gccccagaca attccacacc agccgcggtt
    27541 tccactccgg tgtttgcacc cccgtatggt attgcagcgc ctttccaggg aaacaacacc
    27601 agcacaacag caggagtgtc agcatcattg cgccagccat cggtgccatt tgggcaataa
    27661 atccggcaaa cgaaagacca gaacgtcccc agataagaat attttgcggg ttgccaattg
    27721 gcgtcagtag cgaaccagcg ttgactgcca gcgcctcaaa aataatcagc cgattaaccg
    27781 ggatctcaca cagtcttttt agcgtgatag tcagcggaac aacaataaac agcgcgacat
    27841 cgttggtcag aaaggtagaa agcagcgccg ccgccagcac cataaacatc gccagccgac
    27901 gctccgtagc aaagcggcgc accattttgc gccccagcac atcaaaataa ccgcttaact
    27961 ccacaccttt ggtcagcagc atcaggccgc ttaaggtgat gatggtgtgc cagtcgatag
    28021 cagcaggcca ggatttcggt gcaaagggca cgaaaaagct taatccgata ccaacaagaa
    28081 ttaataactg aaaaaaacga tcgccttgca gcgtgcgtaa aaaaggcagg ctcatttggc
    28141 accgtgtttc tgggtaaaca aacgaaaagc gtcgagcgtc tcttcactaa catggtgctc
    28201 catgccttcc gcgtcgcgac gggcgatttc cggactgacg cccaacacca acaagaaatt
    28261 ttcgactatc tgatgtcgct cgcggctttc ctgcgccagc ttctctcctt ctgccgttaa
    28321 aaacacgcca cgccagggga tcatttcaat cagccccatg gttgccagcc gcttaagcat
    28381 tttagccacc gtcggttgcg aaactcccag acgagcagcc atgtccacct gacgagcttc
    28441 ccccacttcc ctgatcaagt cagaaatcag ctcaacgtaa tcatcaataa gctcgcgccg
    28501 atgcgcctct ctgacctggc ggaacccttc aacgtgctct tccacgttca ctaactgcgt
    28561 cacttttttt gctattggcg tacctgcgcg acgactcatt gtgcttcctc attatggtga
    28621 cgcatacata gcgttctcat ttcgtgcgca cattgtaaac cagagttgcg aaggtacaaa
    28681 aaattaacgt tttagcaata gctatataat atagcctgtg ctatatctgt atgtaatgca
    28741 atcatccctc aaggatcgac gggattagca agtcaggagg tcttatgaat gagttcaaga
    28801 ggtgtatgcg cgtgtttagt cattctccct ttaaagtacg gttaatgctg ctctctatgt
    28861 tgtgcgatat ggtcaacaac aaaccgcagc aagataaacc ttcagataaa tagcggcgtc
    28921 gcggtacgcc gcttcactcc tgcttttatg caggcataac gcgttttggt ctgaaaaaaa
    28981 ccactttttg tcggatttgc aatccccttc gcaaaagatt tgttcgtcag tagttgacct
    29041 gaacggcggc tcgctctatc ttcttgcagc cctgcgtata ttgcggctcg cggatgcgga
    29101 cccctttccc ctcttcacgc actcttgcag gtattgaccc ttgacgccag ggtaagcaca
    29161 tggcgtttgt tacgatagtg gcatatgaat ttaaccctca aagaatcgct tgttacccgt
    29221 agccgggtat ttagcccgtg gactgcgttc tactttttac agtcgctatt aattaacctc
    29281 ggcttaggtt accccttcag tttgctctac accgctgcgt ttacggctat tttgcttttg
    29341 ctatggcgaa cattgcctcg cgtacaaaaa gttctggtcg gtgtcagttc gctggtggcg
    29401 gcttgttatt tcccttttgc tcaggcctac ggcgcgccta atttcaatac attgctggca
    29461 ttgcactcca ccaatatgga agagtcgacc gaaatcctga cgatttttcc gtggtacagc
    29521 tacctggtcg gcttatttat ttttgcgctc ggcgtaatag caatcaggcg aaaaaaagag
    29581 aatgaaaaag cgcgctggaa taccttcgac agcctgtgtc tggtattcag tgtggcgaca
    29641 ttttttgttg ctcccgtgca aaacctggcc tggggtggcg tatttaaact gaaagatact
    29701 ggctatccgg tatttcgttt tgctaaggat gtcatcgtca ataataacga ggtgattgaa
    29761 gagcaagaac ggatggcaaa actttccgga atgaaagata cctggacggt cactgccgtt
    29821 aagccgaagt atcagaccta tgtggtagtg atcggtgaaa gcgcgcgtcg cgatgccctc
    29881 ggtgcctttg gcggtcactg ggacaatacc ccgtttgcca gcagcgttaa cggtttgata
    29941 tttgctgact acattgctgc cagtggctcc acgcagaaat cgcttggctt aacgctcaat
    30001 cgcgtagtcg atggcaaacc acagtttcag gataactttg tcaccctggc aaatcgcgcg
    30061 ggcttccaaa cctggtggtt ttccaaccag ggccaaatcg gcgaatacga tactgctatc
    30121 gccagcatcg ccaaacgtgc agatgaagtg tacttcctga aagaaggtaa ttttgaagca
    30181 gataaaaaca cgaaagacga agcgttactg gatatgaccg ctcaagtgct ggcgcaagag
    30241 cactcgcaac cgcagctgat tgttctgcat ctgatgggct cgcatccgca ggcctgcgac
    30301 aggacacaag gcaaatacga aacctttgtg caatcgaaag aaacgtcgtg ctatctctat
    30361 accatgacgc aaacggacga tttactgcgc aagctgtacg atcagttacg caacagcggc
    30421 agcagcttct ctctggttta cttttctgac cacggtctgg cctttaaaga gcgcggcaaa
    30481 gacgtgcaat accttgccca tgatgataaa tatcagcaaa atttccaggt gccttttatg
    30541 gtcatttcca gcggcgataa agcgcatcgg gtgattaaag cccgccgctc agccaatgac
    30601 ttcttaggct ttttctcgca gtggacggga attaaagcga aggaaataaa tatcaaatac
    30661 ccgtttatat ctgagaagaa agccgggccg atatacatca ccaacttcca gttacagaag
    30721 gtggattaca accatctcgg aaccgatatt ttcgacccga aaccttaaaa acaaaaatcc
    30781 gccccgagag gcggattttt atatcaccaa agtgattaga agcggtaacc aacaccggca
    30841 atccaagtgc ctacgtcaac gctacgaata cggctctgct cgtaagagaa gtccagagca
    30901 acgttttcca tcgggttgaa ctgcagacca gcaccgtagg agaaaccgta gtcgctggtg
    30961 tcgtgtttgt aggtcgggta ttcagtggtc tggaatttac cataacccac acccactaca
    31021 ccgtagatgc ttgcccagtc gttaatgcgg taagccggac cagcagtgat gccgtagtac
    31081 tggtttttgt tgtagtcacc agagcttgca gtacggcttt tctcggtgta agtgaaagaa
    31141 ccgatcacac ccagcgggct gttgtcttct tcatagcggt atttcaggtt gaaaccgccc
    31201 attttgttca tttggccctg agcgtcgctc tgtgcgtaac cgccagttac ggtagaagtc
    31261 gcagctacgg aagtacctgc ggtgaaagcc agaactgcgg ccagtgctga aagacatgca
    31321 atttttttca taaccacctc aaatgtgatt caaataagtc ctaagtttta aatatatcaa
    31381 aaattaatgg gaaactcttc gcgatttgtg atgtctaacg ggccatttca tgtaacagaa
    31441 cgtttccata caccgctatc catctaaatt taaatcactt tttcagagaa ctgcgtaagt
    31501 attacgcatg ttttccctgt cattcatcca gattattcct aatcaccaga ctaatgattc
    31561 catcaatcct ggcgcatttt agtcaaaacg ggggaaaatt ttttcaacaa atgctcaacc
    31621 agcattgggt atatccagta cactccacgc tttacttaag tctagatatt tgtgggagaa
    31681 aggatgcctg gttcattacg taaaatgccg gtctggttac caatagtcat attgctcgtt
    31741 gccatggcgt ctattcaggg tggagcctcg ttagctaagt cactttttcc tctggtgagc
    31801 gcaccgggtg tcactgcgct gcgtctggca ttaggaacgc tgatcctcat cgcgttcttt
    31861 aagccatggc gactgcgctt tgccaaagag caacggttac cgctgttgtt ttacggcgtt
    31921 tcgctgggtg ggatgaatta tcttttttat ctttctattc agacagtacc gctgggtatt
    31981 gcggtggcgc tggagttcac cggaccactg gcggtggcac tgttctcttc tcgtcgcccg
    32041 gtagatttcg tctgggttgt gctggcggtt cttggtctgt ggttcctgct accgctgggg
    32101 caagacgttt cccatgtcga tttaaccggc tgtgcgctgg cactgggggc cggggcttgt
    32161 tgggctattt acattttaag tgggcaacgc gcaggagcgg aacatggccc tgcgacggta
    32221 gcaattggtt cgttgattgc agcgttaatt ttcgtgccaa ttggagcgct tcaggctggt
    32281 gaagcactct ggcactggtc ggttattcca ttgggtctgg ctgtcgctat tctctcgacc
    32341 gctctgcctt attcgctgga aatgattgcc ctcacccgtt tgccaacacg gacatttggt
    32401 acgctgatga gcatggaacc ggcgctggct gccgtttccg ggatgatttt cctcggagaa
    32461 acactgacac ccatacagct gctggcgctc ggcgctatca tcgccgcttc aatggggtct
    32521 acgctgacag tacgcaaaga gagcaaaata aaagaattag acattaatta aatttacatt
    32581 tctgtatggt tatgcataac catacagaat ttctcgctac ttttcctcta cac
//
LOCUS       DAEEUE010000039        32143 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.039, whole genome shotgun sequence.
ACCESSION   DAEEUE010000039 DAEEUE010000000
VERSION     DAEEUE010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32143
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.039"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            1..956
                     /gene="espL1"
                     /locus_tag="KLK84_001954"
                     /pseudo
     CDS             1..956
                     /gene="espL1"
                     /locus_tag="KLK84_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1128..1235
                     /locus_tag="KLK84_001955"
     CDS             1128..1235
                     /locus_tag="KLK84_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009292.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1288..2046)
                     /locus_tag="KLK84_001956"
     CDS             complement(1288..2046)
                     /locus_tag="KLK84_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HBE2009293.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2333..3262
                     /gene="pfkB"
                     /locus_tag="KLK84_001957"
     CDS             2333..3262
                     /gene="pfkB"
                     /locus_tag="KLK84_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HBE2009294.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3363..3653
                     /gene="ghoS"
                     /locus_tag="KLK84_001958"
     CDS             3363..3653
                     /gene="ghoS"
                     /locus_tag="KLK84_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HBE2009295.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3759..4619
                     /locus_tag="KLK84_001959"
     CDS             3759..4619
                     /locus_tag="KLK84_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBE2009296.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4660..5196)
                     /locus_tag="KLK84_001960"
     CDS             complement(4660..5196)
                     /locus_tag="KLK84_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HBE2009297.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFIGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5343..6011
                     /gene="hxpB"
                     /locus_tag="KLK84_001961"
     CDS             5343..6011
                     /gene="hxpB"
                     /locus_tag="KLK84_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HBE2009298.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6174..6764
                     /locus_tag="KLK84_001962"
     CDS             6174..6764
                     /locus_tag="KLK84_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBE2009299.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6897..8287
                     /gene="tcyP"
                     /locus_tag="KLK84_001963"
                     /pseudo
     CDS             6897..8287
                     /gene="tcyP"
                     /locus_tag="KLK84_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(8291..9107)
                     /locus_tag="KLK84_001964"
                     /pseudo
     CDS             complement(8291..9107)
                     /locus_tag="KLK84_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9384..9647)
                     /gene="cedA"
                     /locus_tag="KLK84_001965"
     CDS             complement(9384..9647)
                     /gene="cedA"
                     /locus_tag="KLK84_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HBE2009300.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            9830..12091
                     /gene="katE"
                     /locus_tag="KLK84_001966"
     CDS             9830..12091
                     /gene="katE"
                     /locus_tag="KLK84_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HBE2009301.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            complement(12138..12896)
                     /gene="chbG"
                     /locus_tag="KLK84_001967"
     CDS             complement(12138..12896)
                     /gene="chbG"
                     /locus_tag="KLK84_001967"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBE2009302.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPVNLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(12909..14261)
                     /gene="celF"
                     /locus_tag="KLK84_001968"
                     /gene_synonym="chbF"
     CDS             complement(12909..14261)
                     /gene="celF"
                     /locus_tag="KLK84_001968"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBE2009303.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGHDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(14366..15208)
                     /gene="chbR"
                     /locus_tag="KLK84_001969"
                     /pseudo
     CDS             complement(14366..15208)
                     /gene="chbR"
                     /locus_tag="KLK84_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(15216..15566)
                     /gene="chbA"
                     /locus_tag="KLK84_001970"
     CDS             complement(15216..15566)
                     /gene="chbA"
                     /locus_tag="KLK84_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HBE2009304.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(15625..16974)
                     /gene="chbC"
                     /locus_tag="KLK84_001971"
     CDS             complement(15625..16974)
                     /gene="chbC"
                     /locus_tag="KLK84_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HBE2009305.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANP"
     gene            complement(17059..17379)
                     /gene="chbB"
                     /locus_tag="KLK84_001972"
     CDS             complement(17059..17379)
                     /gene="chbB"
                     /locus_tag="KLK84_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HBE2009306.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(17678..18016)
                     /gene="osmE"
                     /locus_tag="KLK84_001973"
     CDS             complement(17678..18016)
                     /gene="osmE"
                     /locus_tag="KLK84_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HBE2009307.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            18218..19045
                     /gene="nadE"
                     /locus_tag="KLK84_001974"
     CDS             18218..19045
                     /gene="nadE"
                     /locus_tag="KLK84_001974"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBE2009308.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            19275..20162
                     /gene="cho"
                     /locus_tag="KLK84_001975"
     CDS             19275..20162
                     /gene="cho"
                     /locus_tag="KLK84_001975"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HBE2009309.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(20122..20697)
                     /gene="ves"
                     /locus_tag="KLK84_001976"
     CDS             complement(20122..20697)
                     /gene="ves"
                     /locus_tag="KLK84_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HBE2009310.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(20900..21385)
                     /gene="spy"
                     /locus_tag="KLK84_001977"
     CDS             complement(20900..21385)
                     /gene="spy"
                     /locus_tag="KLK84_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HBE2009311.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(21715..22683)
                     /gene="astE"
                     /locus_tag="KLK84_001978"
     CDS             complement(21715..22683)
                     /gene="astE"
                     /locus_tag="KLK84_001978"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HBE2009312.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALVCTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(22676..24009)
                     /gene="astB"
                     /locus_tag="KLK84_001979"
                     /pseudo
     CDS             complement(22676..24009)
                     /gene="astB"
                     /locus_tag="KLK84_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(24006..25484)
                     /gene="astD"
                     /locus_tag="KLK84_001980"
     CDS             complement(24006..25484)
                     /gene="astD"
                     /locus_tag="KLK84_001980"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HBE2009313.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTVGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPLLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(25481..26515)
                     /gene="astA"
                     /locus_tag="KLK84_001981"
     CDS             complement(25481..26515)
                     /gene="astA"
                     /locus_tag="KLK84_001981"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HBE2009314.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(26512..27732)
                     /gene="astC"
                     /locus_tag="KLK84_001982"
                     /pseudo
     CDS             complement(26512..27732)
                     /gene="astC"
                     /locus_tag="KLK84_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            28178..28984
                     /gene="xthA"
                     /locus_tag="KLK84_001983"
     CDS             28178..28984
                     /gene="xthA"
                     /locus_tag="KLK84_001983"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBE2009315.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            29151..29859
                     /locus_tag="KLK84_001984"
                     /pseudo
     CDS             29151..29859
                     /locus_tag="KLK84_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
     gene            29864..30541
                     /gene="ydjY"
                     /locus_tag="KLK84_001985"
     CDS             29864..30541
                     /gene="ydjY"
                     /locus_tag="KLK84_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HBE2009316.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            30556..31263
                     /locus_tag="KLK84_001986"
     CDS             30556..31263
                     /locus_tag="KLK84_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBE2009317.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            31263..31811
                     /locus_tag="KLK84_001987"
     CDS             31263..31811
                     /locus_tag="KLK84_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBE2009318.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            31821..>32143
                     /locus_tag="KLK84_001988"
     CDS             31821..>32143
                     /locus_tag="KLK84_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBE2009319.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTL"
BASE COUNT         8246 a         8242 c         7612 g         8043 t
ORIGIN      
        1 tgctcagacc tgggctgata ggtatgttcg caactgtgca attgctattc tgcccctatt
       61 acctgtggga gttccagggg ctattttcgc gggtattgca tcacgaatgc catttgcccc
      121 tatacatcca tcggcaatgt tgttaataat ggctacaggc cagactcaac agcttattac
      181 attattcaaa cagttgccca tactccctga aaaagaaatc attgaaataa taactgcgca
      241 gaatagcgtt ggtacacctg ctttattttt ggctatgatg aacggacata ctgacaacgt
      301 gaaaatattt atgcaagaaa ttcagtcact ggtagataat cacatcattc atgaagataa
      361 tctggttaaa ttactgcaaa ctaaaagtgc taacgaaaca cctggacttt atatctccat
      421 gttgtatgga ttcgatgaaa taatcgatat ctttctgaat acattaacca ctccaatagc
      481 actaagagct tttaaacaaa aaactggtga tgagtatttt agccatgaaa atacatgatg
      541 gtgagccagg attatacgcc gcaatggaaa ataatcaccc tttgtgtgtc acacggttcc
      601 tctctaaaat taatggcatc gcctttaaat acaagttgag caaagctaac atcatggatt
      661 tattaaaagg agctacagca cagggaaccc ctgcattata catcgccatg agcaagggta
      721 atgaagacgt cgtgttatct tatatatcga cgctgggtgc ttttgcaaaa aaacattctt
      781 ttagtcaaca tcagttattt acactattgg ccgctaaaaa tcatgacaac atgtcagctg
      841 ttcatatagc cattcatcat aagcattata aaactgtaga aacatattat gctgctataa
      901 atgtcatcag ccaaagcctg agttttagtg ctgatgaaat aaagacgtat ttataaccag
      961 ctatttacga tatatatcga tacataattt tatttttttc ataaaacgca gaaacagtga
     1021 ccatgtgcac aacgccaccc gacggctgta ataacaaaaa atcccttgat gcctgtccct
     1081 tttgttacac tccgttatca cgcacaagag atatgcagga cactggtatg ccgactaaac
     1141 gctttgataa aaaacactgg aagatggtgg tggtgctact ggcaatctgt ggcgctatgt
     1201 tgttgctacg ttgggcagca atgatttggg gctgagcagt gacaaacgaa gacagccaga
     1261 ccgcataaca cggtccggca gataatttta catgctgtag cccagcgata aggttgtccg
     1321 acggtcggta tgttccggtg cagactctgg aggttcagag ttccatgtga cgttgtaagc
     1381 caccttcagt ccaaaatgtt cattaatagc aacatttaat gcgctttcgg agttcagcgt
     1441 tgtgtcttcc gcgccaaaga ccgaaacacc ctgcgtaaat ttagcgttgt cagtcaactg
     1501 ccatgcataa gccccggagg catagcccag cggctgggtt tcactggcat tgtcggtata
     1561 tttgtcgtaa cgcacacctg gaccgaattc aaagcggaaa ctgtgtaccg ggccattcaa
     1621 aaactgacga ccataacccg cggtcaacac atcgcgctct cgatagccgt tataacggtc
     1681 tgtcagccag cttgcctgac caaataaata gtcataatca gttaaattaa agcggctacg
     1741 cccgcccgcc gcatattttt ctgaagaacg ctcatcatta gaagaagtat tactggcgtt
     1801 cccccacagc gaccaggcag tggtttgtcc ataccaggtc atggtggtgt cagccgtaag
     1861 tgaggagctt ttcgtattgc ctgattgtgc aagatatcct gcgttcagat taccttcgaa
     1921 aggtttttta gcgctggcag gatcgtccat gacagtaaaa acggaatcat cggcagctgc
     1981 attcagtgac gcaaacatgc cccccgccaa cataacgatg gcaggaactg tcttcaaaag
     2041 cttcatttat caagagtccg tacaacaaaa aaagagacca tcgcggtccc ggaaactttc
     2101 ttaaggatca aagattagcg tccctggaaa ggtaacgaat tataaaaagg cgcgaataac
     2161 ttagcaatgt attcttattt cattttttga ataagcatgt ggcgaaaaca gatttttatt
     2221 tatatatatt tatctgcaaa attttaaata aagctctaat aaatcatatt gttaatttct
     2281 tcactttccg ctgattcggt gccagactga aatcagccta taggaggaaa tgatggtacg
     2341 tatctatacg ttgacacttg cgccctctct cgatagcgca acaattaccc cgcaaattta
     2401 tcccgaagga aaactgcgct gtaccgcacc ggtgttcgaa cccgggggcg gcggcatcaa
     2461 cgtcgcccgc gccattgccc atcttggagg cagtgccaca gcgatcttcc cggcgggtgg
     2521 cgcgaccggc gaacacctgg tttcactgtt ggcggatgaa aatgtccccg tcgctactgt
     2581 agaagccaaa gactggaccc ggcagaattt acacgtacat gtggaagcaa gcggtgaaca
     2641 gtatcgtttt gttatgccag gcgcggcatt aaatgaagat gagtttcgcc agcttgaaga
     2701 gcaagttctg gaaattgaat ccggggccat cctggtcata agcggaagcc tgccgccagg
     2761 tgtgaagctg gaaaaattaa cccaactgat ttccgctgcg caaaaccaag ggatccgctg
     2821 catcatcgac agttctggcg aagcgttaag tgcagcactg gcgattggta acatcgagtt
     2881 ggttaagcct aaccaaaaag aactcagtgc gctggtgaat cgcgaactca cccagccaga
     2941 cgatgtccgc aaagccgcgc aggaaatcgt taatagcggc aaggccaaac gggttgtcgt
     3001 ttcccttggt ccacaaggag cgctgggtgt cgatagtgaa aactgcattc aggtggtgcc
     3061 accaccggtg aaaagccaga gtaccgttgg cgctggtgac agcatggtcg gcgcgatgac
     3121 actgaaactg gcagaaaatg cctctcttga agagatggtc cgttttggcg tagctgcggg
     3181 gagtgcagcc acactcaatc agggaacacg tctgtgctcc catgacgata cgcaaaaaat
     3241 ttacgcttac ctttcccgct aacaaaaaca ttcccccagc attgggggaa tcaccaccaa
     3301 cctgtcggca acgcgtttct ccgactatgc tcaaaagtca tgtgataaca aaggggtgaa
     3361 ctatggccag tggcgatctt gtccgttatg tcataaccgt catgttgcat gaggatacat
     3421 tgactgaaat taacgagttg aataattacc tgactcgcga cggttttttg ctcaccatga
     3481 cggatgatga gggaaatatc catgagctgg ggactaacac ttttggactt atcagtaccc
     3541 aaagtgaaga agaaattaga gaactggttt cggggcttac ccaaagtgca accggcaaag
     3601 atcctgaaat caccatcacg acctgggagg aatggaatag caacagaaaa taaatggttt
     3661 ttgggcaata atcagtctgt ggtgtgcgtt agctcgtgtt tttacaccgc attcttgcgc
     3721 taaccttatg atctggcaga caacatggga gagacatcat gtggcaggca atcagtcgtc
     3781 ttttgagcga gcagttaggt gaaggcgaaa tcgaactgcg taatgaactg cctggcggag
     3841 aagtccatgc cgcatggcat ttgcgctatg caggacatga ctttttcgtc aaatgtgatg
     3901 aaagggaact gcttcccggt tttaccgccg aagccgacca actggagtta ctgtcgcgta
     3961 gtaaaaccgt caccgtgcct aaggtttggg cagtaggcgc tgaccgtgac tacagttttc
     4021 tggtgatgga ttatctccca cctcgtccgc tggatgcgca tagcgcattt attcttggtc
     4081 agcaaattgc gcgtttacat caatggagtg accaaccaca atttggcctc gattttgata
     4141 actcgctctc cacaactcca cagcccaaca cctggcaacg tcgctggtca acgttttttg
     4201 ctgaacaacg gattggctgg cagttggaac tggcagcaga gaaagggatc gctttcggca
     4261 atatcgacgc catcgtcgag catattcagc agcgtctggc ctcgcatcaa ccgcagcctt
     4321 ctctgttgca cggcgattta tggtccggca actgtgcgct gggtccggat ggcccgtaca
     4381 ttttcgaccc tgcctgcgac tggggtgacc gagagtgcga cctagcgatg ttaccgctgc
     4441 atactgaaca gccgccacaa atctatgacg gctatcagtc agtatccccg ctacctgccg
     4501 atttccttga acgtcaaccg gtttaccaac tctacacgct gttaaatcgt gcaagattat
     4561 ttggcggtca gcatttggtt attgctcagc agtcattgga tagattatta gcagcatgat
     4621 atgggttgag gataatggcc gctccgtgcg gccttttgat taaataaacc caagcagaga
     4681 aaagaacaca taacctgcag caatacaaat aaccggcagg atatatagtg agaaaaactg
     4741 caggaaaata gtatggtgcg gaacaacaat acgggactca atttgttcac gcgttagccc
     4801 ctcttcccct ttggcttttt ccagaatgag ttgatcttca acaccttccc gcaagaagcg
     4861 cgcctggcga ctcatccggg caccggaatc ctgcaacgcc agtccgataa aaatcaggat
     4921 gaaaatcacc cagaacataa cgttcacacc accattaaaa ttaggggtcg gggagttata
     4981 ccagaacaga ttcaaaaaag gcgtattcgc ctgcatcata tcgatcatga catgagcaaa
     5041 gtcgagcatg accgcattaa tgccttcctg gttttcctgc cgggtattca taaatttcag
     5101 caaagaaatc aatgtagaga tcaatgcagg tataaaaatc acccaaccca aaatcctttt
     5161 caaaacagca atgcgtccag cttgttgata cgtcatgagt tctccttgat taagacgcgt
     5221 cgtttcactt agtttacctg tagatatctg ttttcgccca ttctttaaag gcgatatgat
     5281 aggcgcttaa tcataagcac ggcttaatac cttacacata atgctctaaa ggagaggttg
     5341 taatgtcaac cccgcgtcag attcttgctg caatttttga tatggatgga ttacttatcg
     5401 actcagaacc tttatgggat cgagccgaac tggatgtgat ggcaagcctg ggggtagata
     5461 tctcccgtcg taacgagctg ccggacacct taggtttacg catcgatatg gtggtcgatc
     5521 tttggtacgc ccggcaaccg tggaatgggc caagccgtca ggaagtagta gaacgggtta
     5581 ttgcccgtgc catttcactg gttgaagaga cacgtccatt attaccaggc gtgcgcgaag
     5641 ctgttgcgtt atgcaaagaa caaggtttat tggtgggact ggcctccgcg tcaccactac
     5701 atatgctgga aaaagtgttg accatgtttg acttacgcga cagtttcgat gccctcgcct
     5761 cggctgaaaa actgccttac agcaagccgc atccgcaagt atatctcgac tgcgcagcaa
     5821 aactgggcgt tgacccactg acctgcgtgg cgctggaaga ctcggtaaat ggcatgatcg
     5881 cttctaaagc agcccgcatg cgttccatcg tcgttcctgc gccagaagcg caaaatgatc
     5941 cacgttttgt attagcaaac gtcaaactct catcgctgac agaactcacc gcaaaagacc
     6001 ttctcggtta atgaccaggg gcagtgatca cgctgcccct ggttctttat ctgaatttgc
     6061 attcaactga cggattaatc gtcaatttaa gagaaagagt tacaccgtca ccacttccgt
     6121 gcactgtata aaaatcctat actgtacgta tcgacagttt agtgagtttt atcatgacgg
     6181 cggaaggtca ccttctcttt tctattgctt gtgcggtatt tgccaaaaat gccgagctga
     6241 cgcccgtgct ggcacagggt gactggtggc atattgtccc ttccgcaatc ctgacgtgtt
     6301 tgttaccgga catcgatcac ccaaagtcgt ttcttgggca gcgattaaaa tggatatcaa
     6361 aaccgatcgc ccgcgctttt gggcatcgtg gttttaccca cagtctgctg gcggtatttg
     6421 cgctgctggc aaccttttac cttaaggttc cggaaggctg gttcattccg gctgatgcgc
     6481 tacaaggaat ggtgctgggt tatttgagcc acatacttgc cgatatgctg acacccgccg
     6541 gtgttcccct gctctggcca tgccgctggc gtttccgctt gcctatcctg gttccccaaa
     6601 agggcaacca actggaacgt tttatctgca tggcattatt tgtctggtcg gtatggatgc
     6661 cccattcatt acccgagaac agcgctgttc gttggtcatc gcaaatgatc aataccttgc
     6721 agatccagtt tcatcggctt attaagcatc aggttgaata ctaaaaaggc aaaaatcacc
     6781 tttctggaat aagcaattcc atttgaatat aagagccagc tcacagttct gttaatcttg
     6841 cgccaacact atgactgcta cgcagtgata gaaataataa gatcaggaga acggggatga
     6901 actttccatt aattgcgaac atcgtggtgt tcgttgtact gctgtttgcg ctggctcaga
     6961 cccgccataa acagtggagt ctggcgaaaa aagtgctggt gggtctggtg atgggtgtgg
     7021 tttttggcct tgccctgcat accatttatg gttctgacag ccaggtactt aaagattctg
     7081 tacagtggtt taacatcgtt ggtaacggct atgttcaact gctgcaaatg atcgttatgc
     7141 cgttagtctt cgcctctatt ctgagcgcgg ttgcccgtct gcataacgca tctcagttag
     7201 gcaaaatcag ttttctgacc atcggtacgc ttttgtttac cacgctgatt gcggcgctgg
     7261 tcggtgtgct ggtcaccaac ctgtttggtt tgacggctga aggtctggtt cagggtggtg
     7321 cagaaactgc acgtctgaac gccattgaaa gtaactatgt tggtaaagtc tctgatcaga
     7381 gcgttccgca gctggtcttg tcctttatcc cgaaaaaccc gtttgccgat cttaccggag
     7441 ccaatccgac gtcaatcatc agcgtggtaa tttttgccgc attcctcggc gtagctgcat
     7501 tgaaactgct gaaggatgat gcgccgaaag gggaacgcgt cttaaccgct atcgataccc
     7561 tacaaagctg ggtgatgaaa ctggttcgcc ttgtcatgca gttgacccct tacggcgttc
     7621 tggctctaat gaccaaagtg gttgcaggtt ctaacttgca agacatcatc aaactgggaa
     7681 gtttcgttgt cgcgtcctac ctcggtctgc tgattatgtt tgctgtgcat ggcattctgc
     7741 tgggcattaa tggcgtgagt ccgctgaagt acttccgtaa ggtatggcct gtgctgacgt
     7801 ttgcctttac cagccgttcc agtgctgcgt ctatcccact gaatgtggaa gcacaaacgc
     7861 gtcgtctggg cgttcctgaa tccatcgcca gtttcgccgc ctctttcggt gcaaccattg
     7921 gtcagaacgg ctgcgccggt ttgtatccgg caatgctgcg gtgatggttg cgcctacggt
     7981 tggcattaac ccgctggacc cgatgtggat tgcgacgctg gtcggtattg ttaccgttag
     8041 ttccgcaggc gttgccggtg tcggtggtgg tgcaactttc gccgcactga ttgtgctgcc
     8101 tgcgatgggc ctgccagtaa ccctggtggc gctgttaatc tccgttgaac cgcttatcga
     8161 catgggccgt acggcgctaa acgttagtgg ctcgatgaca gctggcacgc tgaccagcca
     8221 gtggctgaag caaaccgata aagccattct ggatagcgaa gacgacgccg aactggcaca
     8281 ccgttaattc ttatgctggc aacggtccgt tttgtatagg ggccgttgct ttacttttta
     8341 aatgttccat cgcggcgatg catactgact catatttaaa cagatcgtca gcgaaaaaaa
     8401 tgtccaactt atcaatacat tcctggctca atgaatgtgc ctgtagtgag aagcttaaaa
     8461 cctcataccc gagacgtttt ctaagtgaac tatctggaat actcataata tcttcaagca
     8521 caccacttcc cattcccgga agatttagtg gattaaaaag aagattgata ttaagcattg
     8581 atattatcga aggaaacttt aggagactct catttttttt gaatcgcagc aaaaatgctc
     8641 ccggcaggat gtatataatt tttttcatag tatatagtcc ttaaataatg aataaccaac
     8701 ctatttttta tattgatcaa attcgtcatt ttttcgggtc ggaattcaca ggtaaaatcc
     8761 atcccaacaa aattctcatt aatatctctt atccgaaact cactgccata aagattatta
     8821 attatcactc tatatttaac attgtcctgc agatacgtat caaaactaag tgctggcgct
     8881 cctagctttg aggcatcata gttaatgtta gacatatagg caatcatacg attgaagttc
     8941 gactctgcta tttcatcgca gtcacattga cccgttgata taaagaaagt gagtgccgcc
     9001 atggtagact caagcctttc tttaaaagag tcgaaagcca ggcttttaat atttaaatca
     9061 ccataattac tctgtattaa gtttgtagaa aacatctccc gcctcatatt gttaacaaaa
     9121 ttattatctt atttaaatct aagtcattta caatataagt ttaagagcga cgccacagga
     9181 tgaactatca aaaataactc atcatgatta gcaaaactta accattttaa aataaataaa
     9241 taattaaaga aaaaagatca cttatttata gcaatagctc gtcaaaggca gctttttgtt
     9301 acaggtggtt tgaatgaatg tagcaacgaa atgcagaatt tcaggtcctg taactcccgg
     9361 caaaaccggg aggtatgtaa tccttactca gtcacttccc cttcctggcg gatctgattt
     9421 gcccaacgtt gggcagattc aggcacagta aacgccggtg agcgcagaaa tgactctccc
     9481 atcagtacaa acgcaacata tttgccacgc agcatccaga catcacgaaa cgaatccatc
     9541 tttatcgcat gttctggcgg cgcgggttcc gtgcgtggga catagctaat aatctggcgg
     9601 ttttgctggc ggagcggttt cttcattact ggcttcacta aacgcatatt aaaaattaga
     9661 aaaactgtag tttagccgat ttagcccctg tacgtcccgc tttgcgtgta tttcataaca
     9721 ccgtttccag aatagtctcc gaagcgggat ctggctggtg gtctatagtt agagagtttt
     9781 ttgaccaaaa cagcggccct ttcagtaata aattaaggag acgagttcaa tgtcgcaaca
     9841 taacgaaaag aacccacatc agcaccagtc accactacac gattccagcg aagcgaaacc
     9901 ggggatggac tcactggcac ctgaggacgg atctcatcgt ccagcggctg aaccaacacc
     9961 gccaggtgca caacctaccg cccctgggag cctgaaagcc cctgatacac gtaacgaaaa
    10021 acttaattct ctggaagacg tacgcaaagg cagtgaaaat tatgcgctga ccactaatca
    10081 gggcgtgcgc atcgccgatg atcaaaactc actgcgtgcc ggtaaccgtg gtccaacgct
    10141 gctggaagat tttattctgc gcgagaaaat cacccacttt gaccatgagc gcatcccgga
    10201 acgtattgtt catgcacgcg gctcagcagc tcacggttat ttccagccat ataaaagctt
    10261 aagcgatatc accaaagcgg atttcctctc agatccgaac aaaatcaccc cagtatttgt
    10321 taggttctct accgttcagg gtggagctgg ctctgccgat accgtgcgtg atatccgtgg
    10381 ttttgccacc aagttctata ctgaagaggg tatttttgac ctcgttggca ataacacgcc
    10441 aatcttcttt atccaggatg cgcataaatt ccccgatttt gttcatgcgg taaaaccaga
    10501 accgcactgg gcaattccac aagggcaaag tgcccacgat actttctggg attatgtttc
    10561 tctgcaacct gaaactctgc acaacgtgat gtgggcgatg tcggatcgcg gcatcccccg
    10621 cagttaccgc accatggaag gcttcggtat tcacaccttc cgcctgatta atgccgaagg
    10681 gaaggcaacg tttgtacgtt tccactggaa accactggca ggtaaagcct cactcgtttg
    10741 ggatgaagca caaaaactca ccggacgtga cccggacttc caccgccgcg agttgtggga
    10801 agccattgaa gcaggcgatt ttccggaata cgaactgggc ttccagttga ttcctgaaga
    10861 agatgaattc aagttcgact tcgatcttct cgatccaacc aaactgatcc cggaagaact
    10921 ggtgcccgtt cagcgtgtcg gcaaaatggt gctcaatcgc aatccggata acttctttgc
    10981 cgaaaacgaa caagcagcat tccatccagg tcatattgtt cctggtctgg acttcaccaa
    11041 cgatcccctg ttgcagggac gtttgttctc ctataccgat acacaaatca gtcgtcttgg
    11101 tgggccgaat ttccatgaga ttccgattaa ccgcccgacc tgcccttacc ataatttcca
    11161 gcgtgacggc atgcatcgta tggggatcga cactaacccg gcgaattatg aaccgaactc
    11221 gatcaacgat aactggccgc gcgaaacacc accggggccg aaacgcggcg gttttgaatc
    11281 ataccaagag cgcgtggaag gcaataaagt tcgcgagcgc agcccatcgt ttggcgaata
    11341 ttattcccat ccgcgtctgt tctggctaag tcagacgcca ttcgagcagc gccatattgt
    11401 cgatggtttc agttttgagt taagcaaagt cgttcgtccg tatattcgtg agcgcgttgt
    11461 tgaccagctg gcgcatattg atctcactct ggcccaggcg gtggcgaaaa atctcggtat
    11521 cgaactgact gacgaccagc tgaatatcac cccacctccg gacgtcaacg gtctgaaaaa
    11581 ggatccatcc ttaagtttgt acgccattcc tgacggtgat gtgaaaggtc gcgtggtagc
    11641 gattttactt aatgatgaag tgagatcggc agaccttctg gccattctca aggccatgaa
    11701 ggccaaaggc gttcatgcca aactgctcta ctcccgaatg ggtgaagtga ctgcggatga
    11761 cggtacggtg ttgcctatag ccgctacctt tgccggtgca ccttcgctga cggtcgatgc
    11821 ggtcattgtc ccttgcggca atatcgcgga tatcgctgac aacggcgatg ccaactacta
    11881 tctgatggaa gcctacaaac accttaaacc gattgcgctg gcgggagacg cgcgcaagtt
    11941 taaagcaaca atcaaggtag ctgaccaggg tgaagaaggg attgtggaag ctgacagcac
    12001 cgacggtagt tttatggatg aactgttaac gctgatggca gaacaccgcg tgtggtcacg
    12061 cattcctaag attgacaaaa ttccggcgta aatatggata aggtcacgtc ccctggggag
    12121 tgaccttacc accttactta cacatcaaga taactaccca accgataacc acgctcagca
    12181 attgcatatt tcaacgacgc tgatgtcagc acttccagct ccgttaaccg tgggaaacag
    12241 taagcactct gacggatagt attatcgata aacgccggat gacacatcac ctccagcgaa
    12301 ggatcacccc gatggcttgc gtcatccagc acttgcagga acagtgcctc gctaatctct
    12361 tcaccataaa acgcgctact gaatccctgc gaactgcgca gattaaccgg taaatcacca
    12421 gcgttagata gcggctgacg gtcgatgcgc aacgcaatcc cctcttcagc cgcaaacctg
    12481 gcaacaatcg ggaaaatctg cgggaacata tgcacatgat gatggctatc aagatgcgta
    12541 ggtttgcgtc caaatagctc aatgaaacgc aaatactgac tggcaagctc ctgagtaatt
    12601 tcttccagcg gtaaagcatc ttcttctgcc aactgccaga tccattttcc cagcacacca
    12661 tcgcgggtta accccggcat agctgtcagt ggcttgccca tagtaaggac aaagtgcatc
    12721 cctatggcca gactcggttc atcacgactc aactgcaccg catggtcaat agcctgccca
    12781 ttcaccagcg ccgtcgtcga tgtgacaatc ccattgcgac aggcctcgat aatgccgtag
    12841 ttctgacctt tgcttaagcc aaaatcatcg gcattaacaa tcagtaagcg ttccataatc
    12901 agcctcggtt aatgtgcttt tttaagctct gcgatgcagt cggcaaaatt tggcagccat
    12961 ttctcgtgcg ccagaatcat ctcgcgtgcc agcagctcag catcgcgatc ggaatgcacc
    13021 aacggactaa ggtttagcgc cagtaacaca tcgttaaatt ctccgctaag tgcggcgttg
    13081 ctggcagcaa tctcgaagcc tttaatggtg tgaatcagcc ccatcacttt atcatcgaaa
    13141 tgcgtaatgc gcggatgtgg cgtcgcgcca tcgtgcccca gcttacaggt catttctacc
    13201 gcccagtctg ccggaatatt atcaatatgc ccatgatgcg ggatattaac gtaatgttca
    13261 gcttgcttgt cgttgtagat agcgttgatc acttcgcacg ctgcatcaga gtaataagcc
    13321 ccaccgcgct gttccagttc tttcggctta actttcaact ccgggttttt atacagctca
    13381 aaaagttgtt tctcgacttt ctgtactacc tgcgctcgtg cgccgccttt gtagtattcg
    13441 cccatttcaa tagccagcat ctctttttgc ttgaagtaat aaagcaaata agaacacggc
    13501 aacagattca gagaacgaat taagccttcg ctaaatggca gatcgaaaat atttttaacg
    13561 ccagatactt ttaactgccc ggatgccaca ccatcaagca attcggcaaa gcgcgacttg
    13621 ccatttacca gcacatcctt aatgaacacc atatggttga ggccaaacaa atcgatagat
    13681 aaatcatcgc tgtctttcag catcagaaca tcgtgaataa acatcttcat gccgatcgga
    13741 atattacaca cgccgataaa acgtttaaat ccggtatgac gataaacggc ttcagtaacc
    13801 attcccgccg ggttagtgaa gttaatcacc catgcattcg gacaaagttc ttcgacatct
    13861 tttacgatgt caaaaatcac cggaatggta cgcagacctt taaacaaacc gcccgcgccg
    13921 ttggtttcct gaccaagata accatgactt aatggaatac gttcatccag ttcacgcgcc
    13981 ggtaattggc caacgcgcag ttgggtagta acgaaatcag catctttcaa tgcttcgcgg
    14041 caatccagcg ttttataaag cttcatcggg acgccagcgt tatcaatcat ccgttggcag
    14101 agatcgaaaa taatatccag tttagctttg ccaccctcga catccaccag ccataattcg
    14161 ctgaccggca attcgtgata acgcttaata aatccttcca gtaactccgg ggtatagctg
    14221 ctcccgccac caatagtgac gacttttaat ttctggctca taatttctcc cttcagtaca
    14281 gaatactgat atctggcata tctgcccccc cggacataaa taatccagca acaggacaga
    14341 tatgtgaatt gtcaggtata acgacttact gattaaattc agtcaatttc ttacgatatc
    14401 tcttaggcgt aaaggacgtt aatttcttaa acgttttaat aaacaaactc gggctactat
    14461 aaccggcctc aaacgcaata tccgtcacgg aatagttggt catttccagt tgtttttttg
    14521 caaaattaat acggatctca ttaataatct gcattggtgt tttgccataa tatcgttgag
    14581 tcgctcgcgt caaatattcc tgtgatttgg ctgacaacgt caccatattc tccagcgccg
    14641 attcactaaa ctgctcttta tcatgcatct tttctaccgt acttttcagc cactacggta
    14701 catcatcaat cacctgttct tcgcggtaat gacgtaaacg gttaatgaca taaaaagtaa
    14761 ccatctcaac aaactcttcc agccctgttt cgcggaaatt taatgaagaa atcactgttt
    14821 ccacataggt gagaaaagcg ttattggtcc ggtatacctg cgaagcaaca aagcaataag
    14881 gcaacaacgg caggtaatgc tgctcaaaaa agcgtttact gatcccaacg ttcaatatgc
    14941 gcgtggcacc aaactcataa aaactttgat ggtgcgaacc taacggaata aaaacaaaat
    15001 cgccccgttc cagtaacacg cgcttaccgt taatctcctg gaaataacgc ccggttaata
    15061 ctagagtaaa ttcataatag tcgtgctgat gcagtccgct gatactctca gttttgttat
    15121 agataaacac atggaagttt ttgccattaa acaactgctg ttctcgggca gtggcaattt
    15181 ccggcgcgtt aatcactggc tgcatcatcg actccttatg ccttcagttt ttcatgaagc
    15241 tcaattaatt cagtaatcag ttcacgcgca agcatggacg tcattaaatg atcctgagcg
    15301 tggaccagca ccagactcac tttcatctta ccttcgcccg catcgccttc aatcagtttc
    15361 gtctgtacca gatgcgcttc attcaatgcc atccgtgact gatccatcat ggctttcgct
    15421 gcggcaaaat cgccctgctt cgcctgtttc aatgcggcat acgccaggct acgcgcttgt
    15481 ccagagttga tgataagccc catcaccact tcttccagct cttcagcttc tgtttgcgta
    15541 tcaggaatat tatcgagatc catcatacat cgttcctctc tcttcttacc ggcaagatta
    15601 cccgtaccgg catcgattaa aatttcaggg gttagcgata tcttcttcgc tctcttcttt
    15661 atcaatcgca ttttgtgctt tgttagccac cacaacaaag ggcagataaa ttaacgttgc
    15721 gatgccaagg ttgaagagtg cgaccagcaa tgcggcgacg ctaccgttgg tgttaaagaa
    15781 ggctcccaga ccggttggca tggtccacgg tgcaatattg gtcaccggag gaatgatgcc
    15841 catgtagtac gctgcgaggg tgattgccgc cagaatcggt tgtaccagta caaacgggat
    15901 aaacatcacc gggttcatga taattggcag accaaacaga atcggttcgt taatctggaa
    15961 gatgccggac ggcagcgcca gttttgccac ctgacgataa tcagcacggc gagaggcgat
    16021 aaagatagcc aggatcaggc ctaaagtcgc accactgccc ccaaggaaaa taaaggagtc
    16081 cagcatcggc ttggcccaga tatggaaggt cttaccggct gccagcgccg cttcgacgga
    16141 accatattgc tgataggtcg cgatattttc cagcgcccac ggcgtcataa tgccgttgtc
    16201 cagtgcggtc agcgccagcg cgccatgaat accgaagaac cagagcagtg gaacaaagat
    16261 cacataggcc cagcccacca cgctacccaa cgatgccagt ggggttgaga tggtatccat
    16321 aatgatctga tggaagttgg tgccccaggt attcaacgcc caggcaataa tccccatcac
    16381 ggaaagaata ataaagccgg gaattaatgc cgagaaagaa cgcgatactg aagcaggtac
    16441 gctgtcgggc agtttaatga cccaattgcg gcggacaata aaggtaaaca tttctgccac
    16501 caccaggcca ataataatcc cggagatgat attcgcccca cctaaccagt ttgcgccaac
    16561 cgcataggcc tcaccgacac tatatggggt gacggtcata aatgctgcaa cggataacaa
    16621 cccagccgcc agcgcatcga ctttacgctc ttctgccagc gccatgccaa taaagaacgg
    16681 tgccattaaa gacattattc ctaatgttcc gttatatacg ttgccgccaa tacctttcag
    16741 accattaagt gtttcaatgg ttgaggcgtc gaggcgaata cctaaggaat aaaaaaacga
    16801 cccctcccca aagcttagaa aaacgttgtt aattaataca aacatggccc ccgcaagggt
    16861 taacggcatt aagcgaataa agccattttt gattgcatta acgtgtggct gctttcctat
    16921 tttaactgca aaagggagga gtaccttttc aagcgatgca ataacattac tcatagaaaa
    16981 atacccttaa aaaccgcaat ttaaatatta cggtattgat ttatgaaata actctttgac
    17041 gggaaaattt aaaataaatt aatttgctgc ggctttttta atcgctgcaa ccgcagcctt
    17101 aagcacgcct aaaccatcga ctttgccata aagcagcgag tcaattactt caaccggttt
    17161 gttgggtaac aaacgctgga tttcgggcaa catataagca atctgcggcc ctaataacac
    17221 gacatcggca ttctgacctt tttcaccagc cagtgtttcc ggaaatgctt caataatgac
    17281 cggaacttca tatttttctg cctgtgcgcg catttttgat accagtaaag agatagacat
    17341 gcccgcagaa caaaacagat aaatgtgttt cttttccata aaactgccct catcgacgat
    17401 tatctgtcag ccagacactc cgcaagcctt gacctgcttc catgctctgg gtaacttgcg
    17461 aaaccaacat gacttttttt gtggctgaaa ggagtatacg gtatagacct gagcaatagt
    17521 atttctttga ccttataaat tgtttctcct tgacctgtgg ttatgaccct cttcacattt
    17581 cgggcaaata attcgcgaag ataattaaga actcaggcca aaaaaaaccg gcgcaatggc
    17641 cggtttccgt tgttactcaa gctttcagac gaattgatta cttcgcagcc tgtggatcag
    17701 tgtcgtattc agcacaggtc tgataaccgg agttgatgac atgtccggta tcatctaacg
    17761 cgacaaagta ggtttctgct ttaccatcac gttgacccag gatgtaggtc tggcaagtac
    17821 cgcgagcatg gatcatgctc acttcagacg aaggtttacc cgcaatttgt gcaacctgcg
    17881 cccggctcat gccttttttg acgtctttca ccacaggctg tacaaactgg tctttggtac
    17941 gatcataagc tgtacaaccc gccagcatgg ttaataccgc cgctgcactc agaattcctg
    18001 ccatattctt gttcatattc cgtcctcttg tttatcagcg tgttagataa gcctggaata
    18061 cattgggcgc tttttcaagc ccgtgaacga aacggctccg ctttcagagg attcctgtat
    18121 gacgttttaa ccaccattca gcccgctgtc gcttgtcgtt tcagtagcaa cgggttagct
    18181 ttaaggaagt tttgtctttt ctgtctggag gggttcaatg acattgcaac aacaaataat
    18241 aaaggcgctg ggcgcaaaac cgcagattaa tgctgaagag gaaattcgtc gtagtgtcga
    18301 ttttctgaaa agctacctgc aaacttatcc gttcattaaa tcactggtgc tcgggatcag
    18361 cggcggtcag gactccacgc ttgccggaaa gctgtgccag atggcgatta atgagctgcg
    18421 ccaggaaact cgcaacgaat cactgcaatt tattgccgta cgcctgccct atggtgttca
    18481 ggctgacgaa caagattgcc aggatgccat tgcctttatt caaccggatc gcgtattaac
    18541 cgttaatatc aagggcgcgg tattggctag cgagcaggca ttgcgggaag caggcattga
    18601 actgagcgat tttgtccgtg gcaatgaaaa agcgcgtgag cggatgaaag cacaatatag
    18661 cattgcgggt atgaccagcg gtgtcgtggt gggcaccgat catgcagcag aagccattac
    18721 cggattcttc actaaatatg gtgacggcgg tacggatatt aatccgctgt atcgtctcaa
    18781 caaacgccag ggtaaacagt tactgacggc attaggttgc ccggaacacc tttataagaa
    18841 agccccaact gccgatcttg aagatgatcg cccttctctg ccagatgaag tggcactcgg
    18901 cgtgacctat gacaatatcg acgactatct ggaagggaaa aacctacctg aacaggtcgc
    18961 cagaacaata gagaactggt atctgaaaac cgaacataaa cgccgtccgc caattaacgt
    19021 tttcgatgat ttctggaaaa agtaataatt tgcacctatt ggattgtgcg aaaaagagta
    19081 atttgttcac gccggatgcg gcgtgaacgc cttattcgac ctacaaaact atgcaaattc
    19141 aatacattgc aggagtcgaa taggcctgac aggcgtagca cgtcagacgg tgtaaccttt
    19201 gtcatcaacc cgcctctttt ttaatcgcct cccgcctgtt acactggata gataaccagc
    19261 attcggagtc aacagtggta cggcgtttaa cttctccgcg gctcgaattt gaagctgcgg
    19321 caatttatga atatcccgaa catttacatt cattccttaa tgacttaccc acccgaccag
    19381 gggtgtatct gtttcatggc gaaagtgaca ccatgccgct ctatatcggc aaaagcgtta
    19441 atatccgcag tcgggtgctt tctcatttac gcaccccgga tgaagccgcc atgctacggc
    19501 aatcccggcg aattagctgg atctgcaccg caggcgaaat cggcgctctg ctccttgaag
    19561 cgcgattaat caaagaacaa cagccgctgt ttaataaacg gttgcgccgc aatcgccagc
    19621 tctgtgccct gcaattaaat gaaaagcgcg tcgatgtggt gtatgccaaa gaggtggatt
    19681 tttcacgagc ccccaacctg tttggcctgt ttgccaatag gcgcgcagct ttgcaagcat
    19741 tgcagagcat cgctgatgaa caaaaacttt gttatggcct gttgggactg gaaccgttaa
    19801 gtcgcggtcg tgcatgtttt cgttcagcgc taaaacgttg cgccggagca tgctgcggta
    19861 aagagagcca tgaggaacat gcgctacgct tgcgccagtc tctggagcgt ttgcgagtgg
    19921 tgtgttggcc ttggcaaggt gcggtggcac tgaaagaaca gcacccggaa atgactcaat
    19981 atcatattat tcaaaactgg ctgtggctgg gggcggttaa ttcgctggaa gaagcgacaa
    20041 cgttaattcg gacacccgcc gggtttgatc acgacggtta taaaattctt tgtaagccgc
    20101 tgctttccgg taactatgaa attactgaac ttgatccggc gaatgaccag cgagccagtt
    20161 gatttcactg aacaacagtt taccttgcgg ttgtaaaagc cgcagggtgt gcctgccatc
    20221 gaaccagcac gcgccctggt cggtagtgag cagtttgtcg cccagttgcc acgccccgtt
    20281 aatcacaaac accaccccgc cgcgtgagcc aaaggtagta aacgtacgtt cggcaattct
    20341 gactttcgcc ttacagacat ccaggcgcgt cataatattg aaatccatcg acatctgccc
    20401 tgccgtcagt ttcgctttta ccacctggtc cgccgtaaag gcaaaaggct gtaacggttt
    20461 taaggtatgg ttaaagcggt ctgcgctttc aaggagcatc tcgccgcctt ccagcaacgt
    20521 cactatcctt tccatgccgg gaaacaaaga aaactcccca ttcgccgcga tggatgcaat
    20581 gctggcacgc cagtaaaaat cacgttttgc cggtgggaaa gtgcaaattt cgcgcgtttc
    20641 accggcagcg tttcgccaca gattcaccga cattttacgc atatcaaagt attccatact
    20701 cgctcccggc ccgttctttt cattgttatc gttcaggtaa ccgtggatga ttatcggcaa
    20761 ctcgggtgat ttacgttagt ggtgatcagg ctttttttac aactgtttga gaagagaaaa
    20821 gaaaaccgcc gatcctgtcc accgcattac tgcaaggtag tggacaagac cggcggtctt
    20881 aagttttttg gctgaaagat tattcagcag ttgcaggcat tttacctttt gccgctggac
    20941 gttctgtcag acgcttctca aaattagcat taaattgctt tttctgttcc ggcgtcagga
    21001 tgttgtaaat tttgttctgg gtttccatgt gcgccagcat gttagctttg cgctgttctt
    21061 ccatttttgc gatctgcgct tcagcttttg ctttatcgaa ggtatcgctg gcaatgatgt
    21121 catgcattgc gcggcgttct tccagcggcg gacgtttcat ctggtcacgc tggcctttca
    21181 tgatttcgtg gatctgctgt ttctgcgcgt cggtcaggtt caggtctttg aacatcatgt
    21241 cctgatgcgg accgaacttg cctttgtggt gcatcatcgg cttcgcgtca gccggtgctg
    21301 cggtagtggt gtctgcggca tgggccaggt tagccgcgcc aagagccagg gtagaggcaa
    21361 caaacagtgc agttaattta cgcatattct atatccttcc tttcagttat ttattacggc
    21421 tttcttaagt agcgtgccgt gttgacgaga ttaactttac tgactttagc gtcaattaat
    21481 cagagcaacg gtaaaacaat gaaagtgtaa aaaacacttt tgcgccaatt atggagaaaa
    21541 aaagaaaatt tgatggagag tgatgagaga atattacaac acgatgattt tgcagagatt
    21601 atgaagaact ataccggatg actggtgata aataaagcaa ataaccagga ttaatctgta
    21661 ttaatttata agaaagcaac ttaatacccg cagaatgatt tctgcgggta agtattagct
    21721 tattttttcg agcattaatc ccgcgcgtaa tcccaacgct accaacggat tagggaataa
    21781 cacatactct acatcatggg ttacggtaaa acgttcctct ccgtcctgcg ccagcaatgt
    21841 tcctttctca aacggcataa aattcagcgt gtcacttgcc atatgcattt cgaaggacgg
    21901 cgagtggcga gtaatttgcg aaaccacccg ataacggagc ggcggtgttc tcacgatacc
    21961 gacactctca ccagatagca gcgcagcaat tgcgctggca gttactgcaa actggcgaag
    22021 atcgttttgc ccaaagggca acgctttgcc aagttccaac gtacagacca gcgcgccaaa
    22081 atgtctgacg ctgaaatggg taaacgtacc accaggctcc tgatggaaca ccagcgcctc
    22141 cagccccgcc gcacccagcc acgtcagaaa tttctcgtcc cagggaatgt cgcgttgcgg
    22201 taatacaccg aactgcggat gcaaggagcc acgaattgcg gtatgtagat caaggtgcca
    22261 acgcacagat tctttgccct ggtcataaaa atcttccagg cactgttcca gttcgcgcgc
    22321 ccgacaggtt tctccgcttt cagcaaatag ctgccaacga ccgccaaaca ttcggttcat
    22381 atcgctatgg caataacgtt tcccttgctt cagcgcagga ggattcccga ggatcaccag
    22441 caaccgccaa cgtaacggga tctcgccgtg agatatcgcg ccaagcaacg cgtccagcat
    22501 ttccacaggt gccgtctcat taccgtggat ccccgcagaa atcaccagcg cgccttgcgg
    22561 tggcgttaat ggcgtcagtt ccagcacacc atcgcccagc cagcgccagc gaacgccgtt
    22621 gatttctcgc tcggtgataa ccggtttttt acccgttaag gtcagagcaa gaaaattatc
    22681 cattgccccc tccctcgcgc tggaacggat aaaccgaacc gagattcagt aattggctca
    22741 atacatccag tgcttcccgc ccttcgcgca gcaattgcgg gtcggccaga tcggcagcag
    22801 taagacgatc gcggtagtaa cgatccaccc agtcattgag cacattaaac aaggtatcgt
    22861 tcatcatcac cgccggattc accgcccggc gttcttcttc tgtcaatacc ccccgcaacc
    22921 gcaggcacgc cgggccgccg ccattcgcca tgctttcacg taagtcaaag acttttagtt
    22981 cactaatcgg gttgtcagcg gcaaggagtt cattgagata accccatact ccggcgtgct
    23041 cccgacactc ctgaggcagc accaacatca tggaaccatc atcgcggctc aacagttggc
    23101 tgttaaacag ataggtagac accgcatccg acacggaaac ctgagttgcc ggaacttcta
    23161 tcgccataaa accgttcacc cgcgtgccag taactgtgac tggcgagcga acgcctgttg
    23221 gtggcaaaac agcacctggc ggttactcac ggcaatcacg tcattatgaa aaacgctctg
    23281 gtcgataacg tccgggtttt gctgggcgaa aatcacctgt tggggattca cctgattaag
    23341 ccttgccacc gcctcgctgg cttcgcgagt ctgtcgcgcc ggataacggg aaggccgggt
    23401 atcattgcct tcttctcgcc cgtagacaaa aagtagcata cccggttcac cgtaatgacc
    23461 gccgagacga ttgtggtttg ccgccccctc atcaccgagc aacgctacct gcggcaacgc
    23521 cgaatggacg ctaaatttct cttcgtcctt aaaaatcgct tttaacagcg attcagtgac
    23581 gggcgcttcc agcgaacggt gaaatttatt gttcaggttg gcaacggtga gatgcacttt
    23641 gccatccagc gtatcggcag atggcgcgat cgttgccgca ttggctaccc acattggcga
    23701 agcggaactg acgctggaaa gccagtgcgg tgcctggcgt gcaacttttt ccagtacctg
    23761 ctcatcgcta ccactgaatc ccaactgacg cagcaccgga ataaacggac gctcgtgcgg
    23821 cgggatcacg gcctggggga atcccgcatc ggcaagggct ttcattttca gtaagccctg
    23881 ttttgccgcc agtcgcgggt tagacacctg aaaacggtga cgggtagagg cttcattacc
    23941 aaacgacagg cccgcgtaat gatgcgtcag ccctaccagc ccgtcgaaat taacttccca
    24001 ggcgttcatc gcaccacctc atcggaaaaa tccagcccgg ggttaagcgt ggcgggcaat
    24061 gttaacgagt ccgactccag gctcgccata ggccatgcgc agtaatctgc ggcataccag
    24121 gcgctggggc gatggttacc ggaagcacca atgccgccga atggggcggt actggcagca
    24181 ccagtaagcg gtttgttcca gttaacaatc cccgcccgcg cctccagcaa cagttgatcg
    24241 aatttttccc gctcggggga aaccagaccg caaaagagtc cgaagcgagt gttgttcgcc
    24301 atccgaatcg cctcatcaaa agtatcataa cgccagacgc gcaaaagcgg cccgaacacc
    24361 tcttcatccg gcacaccgcc aacgcccgtc atttcaatga tccctggtgt cagcaacgat
    24421 gtccctgctt gtaataagcg cggcgcaagc agggttcgtc cgcccatcgc ttccagttgc
    24481 tgccaggcag taaccacctg ctgtgcggcc tgttcagaaa tcagcccgcc aataaacggc
    24541 tgcggttcgt catcccagtt gcccggcgtt aatcgctggc tgacggcaac cagactagca
    24601 agaaacgcat cgccctgcgc tccgcttttg agcaataaac ggcgggcgca ggtgcagcgt
    24661 tgcccgactg tgacaaacgc cgactgaatg gtcagatgga cagccgcgtc gatatccgcc
    24721 acctcatcga taattagcgg attattaccg cccatctcaa gggcgagaat tttctccggc
    24781 tgaccggaga gctggcgatg cagctggtag ccggtattgg cgctaccggt aaacagcaaa
    24841 ccgtcgagat cctccagcgc actcagcgcc tgacccgttt cacgcccgcc ctgcaccagg
    24901 ttcagcacgc ccggcggcaa gccagcctgc tgccataaac gcattaccgc ttcgccactc
    24961 cacggtgtca gttcgctggg tttaaagata atggtgttac ctgccagcaa tgccggaacg
    25021 atatgtccgt tcggcaaatg accagggaaa ttatacggcc caaacaccgc cagcacgccg
    25081 tgcgggcgat gtcgcaggct cgccgcaccg tccggcattt cactacgctg ctcgccggta
    25141 cgaacgtgat acgccttaat tgatatcgcg attttattga tcatcgccgt cacttcggtt
    25201 gccgcttccc agcgcggctt acccgtttct ctggcaataa tcgcggttaa ttcggcttta
    25261 ttgctttcca gcaatccggc aaagcgttcg acaacgacct gacgttcagc caatgagagc
    25321 cgcgcccagc gcggaaacgc cgcacgggct gcccgacaag cctgttcgac ctgagcggca
    25381 tcggcatcat tgccttgcca taacacctcg cccgataccg gattacgctt cacacgcgat
    25441 gcgccctggc ccgttatcca gtcaccgtta atccataaag tcatgctgtt ttctcctctg
    25501 cgcacaggcg caccagacga acgcgatccc ctgcgtggca tttgagggca tccagttgtg
    25561 cggcggttaa aatcaaacgc tcggttgccg gatcggtacg cgccagcacc acgcggaaat
    25621 ggtgataatt ttcattggcg accaggcagg ccggaaaatc gccctgcgca ggctgccctt
    25681 ctgccacttc caccagccga cttttacgga tggcgcgcac gcggtcgatg tcacactcaa
    25741 gcgttggccc gccgtcgaag atgtcgatat agttgcggta acgaaaacct tctttctcca
    25801 gcaccgcgcg ggcaggcgcg gtttgcggat gtacctgacc gatgacgtcc tgggcttcct
    25861 gggataaaaa gtgggtatag atcggatgtt tcggcatcag ttctgcaata aatgcctttt
    25921 gcccggtgcc gcagagaaaa tcggcgcggc taaaatccat cgaaaagaag cgtttaccga
    25981 ggctttgcca gaacggtgaa tagccgtgtt cgtcaatcac cccgcgcatt tcagcaacca
    26041 ctttgtcatt aaacttgtcg cgaaaagccg ccataaacat aaagcgcgat ttcgacagca
    26101 aatagccgtt gccctctttg cgccagtccg ggtcgagaaa cagcgtgcac agctcgctgc
    26161 tgccggtgtg atcgttacta agaaacagcg tcggcaatgc gttatagaca ttcagctctt
    26221 ttgaggcgtg aaccaacgtg ccgacgcgat agttgtacca gggatcgttc agcccaaccg
    26281 ccacctcaat agcgcaaatc cccgccacgg tgcctgtctc gctatcttcc agcacgaaca
    26341 catagccctg ctcgcttttg ggcagttcac cttgccaggt tttgattgcc ctttcgatac
    26401 gcgccgaaag cgtggcttca ttggcgggaa gcgacgtcag gccgccgccc gttttgctgg
    26461 caagctgcat cagcgccgag acatctgagc gctcaacggg acggatgacc atcatgatga
    26521 acctcggcta acaaagtgtt cgcaagcagc tgcaaagcga tccagtccgg tcgtcacctc
    26581 ttcttcgctg acattgagcg caggcgcaaa acgcaccacg ttgccacccg caatcagtac
    26641 catcacgcct gctttcgccg cttccagaga gatctgtttc gcttgcccgg cgtaatcggc
    26701 attcagtaca cagccaatca acaaacctaa gccgcgaact tcactgaaca aaccatagcg
    26761 gtgattaata gtattaagac gctcaacaaa ccagtcgtga cgctgtttaa cgccattaag
    26821 catctctggt gtgttgatga gctccagcac tttgcctgct accgccgagg ccagcggatt
    26881 accgccatag gtggtgccat gagtgccaac ggtcatcacg cgggcgcact cttcggttgc
    26941 caacaacgca ccgaccggga aaccgccgcc cagcgctttg gcggtggtta acagatcggg
    27001 cgtcacgccg tagtgcatat aggcatacag ctcaccagtg cgcccgacgc cggtttgtac
    27061 ctcatcaaaa atcagcaagg cattgtggcg gtcacacaat tcacgcagac cttgtaaaaa
    27121 cgcgttgctg gctggcacca caccgccttc gccctagatg ggctcgacaa tcaccgcaca
    27181 ggtggcgtcg tcaatcagcg cgctggcaga gttaatatcg ttatatgcag catgacgaat
    27241 atccgccggc agtggcgcaa aatcctgtga ataggctggc tgcccacccg cactgacagt
    27301 aaacagcgtg cgaccatgaa acgcattttt gaacgccacg atgccactct tatggctgcc
    27361 gtagcggtcg tgagcgaatt tacgcgccag ttttagcgcc gcttcgttgg cttccgcacc
    27421 ggagttacaa aagaagacgc gatcggcaaa cgtggcgtcg atcaattttt tcgccagtcg
    27481 cagtaccggc tcgttggtgt aaccgttgcc ggtatgccag aacttactcg cctgttcgtt
    27541 cagcgcttca cgcagttccg gatgcgcatg gcccagcgcg ttcaccgcaa tgccacccgc
    27601 gaagtcgata tactctttcc cctgctgatc ccacaagcgc gaaccttcgc cacgtaccgg
    27661 tataaagggt gccggagcgt aaacaggtat catccattca tcaaagtttt cacgcgtaat
    27721 tggctgagac atagcgacct ctacagtaaa taattcgtta tttgcatgtt aacaataagt
    27781 aatgtttgcg ctgtaaatgt agattgcagg gttcgtgcca gccagtgata aaagtgcata
    27841 aacggcggag gctaactgga aatcaaggag ttataaccaa accatatgca tttaaagtgc
    27901 atataaagtg aatacgtttg cgatgtgggt gaataaaaag aataaaaaac gcaatgttat
    27961 gcagaagtaa aatataattc tagaattgtg atcattgacg aaatttactg gaaattactg
    28021 cgccattctg acgcagcgcg caccaaaagc gggcattttt tgcgccatca ttgacatcat
    28081 taacaaccat cgatcaaatc acttaacaac aggcggtaag caacgcgaaa ttctgctacc
    28141 atccacgcac tctttatctg aataaatggc agcgactatg aaatttgtct cttttaatat
    28201 caacggcctg cgcgccagac ctcaccagct tgaagccatc gtcgaaaagc accaaccgga
    28261 tgtgattggc ctgcaggaga caaaagttca tgacgatatg tttccgctcg aagaggtggc
    28321 gaagctcggc tacaacgtgt tttatcacgg gcagaaaggc cattatggcg tggcgctgct
    28381 gaccaaagag acgccgattg ccgtgcgtcg cggctttccc ggtgacgacg aagaggcgca
    28441 gcggcggatt attatggcgg aaatcccctc actgctgggt aatgtcaccg tgatcaacgg
    28501 ttacttcccg cagggtgaaa gccgcgacca tccgataaaa ttcccggcaa aagcgcagtt
    28561 ttatcagaat ctgcaaaact acctggaaac cgaactcaaa cgtgataatc cggtactgat
    28621 tatgggcgat atgaatatca gccctacaga tctggatatc ggcattggcg aagaaaaccg
    28681 taagcgctgg ctgcgtaccg gtaaatgctc tttcctgccg gaagagcgcg aatggatgga
    28741 caggctgatg agctgggggg tggtcgatac cttccgccat gctaatccgc aaacagcaga
    28801 tcgtttctca tggtttgatt accgctcaaa aggttttgac gataaccgtg gtctgcgcat
    28861 cgacctgctg ctcgccagcc aaccgctggc agaatgttgc gtagaaaccg gcatcgacta
    28921 tgaaattcgc agcatggaaa aaccgtccga tcacgccccc gtctgggcga ccttccgccg
    28981 ctaatttagc agctctcctg gctcaaactg ggtcaggaga attaaccttg agaaaaatca
    29041 acaaactgtc agtaatgatt tgttgcctgc cgtcctttgt tataccgtca ctgcgttttt
    29101 agttgtctga ccacttctct attatcaagt ttgatatagg aaactccacg atgaacgctg
    29161 agcgtaactt tctgtttgcc tgtcttattt ttgcgctggt catttacgct atccacgctt
    29221 tcggtttatt cgatctgctc accgatttac cccacttaca gacactcatt cgccagagcg
    29281 gattgttcgg ttatagcctc tatattctgt tattcatcaa tgccccctct ttctgttacc
    29341 aggaagcata ctggtgatcg ctggaggaat agtttttggc ccgctcttag gacacaactc
    29401 tcattaattg ccgcaacgct ggcctcctcg tgctcattcc tgctggcgcg ctggctggga
    29461 cgtgatttac tgctgaaata cgtgggccat agccatacct tccaggccat tgaaaaaggc
    29521 attgcgcgta acggtattga ttttcttatt ctgacccgct taatcccgtt gtttccttac
    29581 aatattcaaa attacgctta cggattcacc accatcgcct tctggcctta tacccttatt
    29641 tcggcactca cgaccctgcc cggtattgtt atttataccg tgatggcaag cgatctcgcc
    29701 aatgaaggca ttacgctgcg ctttatttta caactctgtc tggcgggcct ggcgctgttt
    29761 attctcatcc agctcgcaaa actctacgcc cgacacaaac atgtggatct gtctgcttcg
    29821 cgccgcagcc cacttactca cccaaaaaat gaaggataga acgatgtcgc aacattattc
    29881 agtgtcatgg aaaaaaggac tggctgcact ctgcttactg gctgttgccg ggcttagcgg
    29941 ctgcgatcaa aaagagaatg cggcggcaaa agtggaatac gacggacttt cgaacagcca
    30001 accgctgcgc gtcgatgcca ataaccatac ggtaaccatg ctggtgcaaa ttaatggtcg
    30061 tttcctcacc gacgacactc gtcacggtat tgtgtttaaa gatggctcca acggacataa
    30121 atcgctgttt atggcttatg cgcccccgaa agcattttat gaagccctga aagaggcagg
    30181 tggtacgccg ggcgaaaaca tgacgatgga taataaagaa acgactcatg tcacaggcag
    30241 caaactggat atttcggtca actggcaagg ggcggcaaaa gcgtattcct tcgatgaagt
    30301 gattgttgac agtaatggca agaaactgga catgcgcttt ggcggtaatt taacggcagc
    30361 agaagagaag aaaacgggtt gcctggtgtg tctggatagc tgcccggtcg gcatcgtcag
    30421 caatgcaaca tacacttatg gtgcggtgga aaaacacggc gaagtgaaat tcaaaggcaa
    30481 tgcctcagtt ctcccggcgg ataacacgct ggcaacggtt acctttaaaa tcaccgaata
    30541 aagccaagat aaaggatgat gaagatacaa tcgcgtaaaa tctggtacta ccgtataacc
    30601 ctcatcatcc tgttattcgc tatgctgctc gcatgggcgc tgcttcccgg cgtccatgag
    30661 tttatcaatc gcagcgttgc ggcgtttgcc gccgtggacc aacagggtat agaacgcttt
    30721 attcagtctt acggcgcact ggcggcagtt gtctcgttct tgttgatgat tttacaggcc
    30781 attgctgcac cgctacctgc gtttttgatc acctttgcca atgcgtcgct gtttggcgcg
    30841 ttctggggcg gcttgctgtc gtggaccagt tcgatggccg gcgcggcgct gtgctttttt
    30901 atcgccagag tgatgggccg cgaagtggtg gaaaaattaa ccggcaaaac cgtgcttgac
    30961 agtatggacg gctttttcac tcgctacggc aaacacacca tcctggtctg tcggttattg
    31021 ccttttgtcc ctttcgatcc aatcagctat gctgccggtc tgacttcaat acgttttcgc
    31081 tcgtttttta tcgccaccgg gcttggtcaa ttaccggcga ctattgttta ttcctgggca
    31141 ggcagcatgt taacaggcgg tactttctgg tttgtcaccg aactgtttat tctgtttgcc
    31201 ctgaccgtgg tgatttttat ggcgaagaaa atatggcttg agcgccagaa gaggaatgac
    31261 tgatgggttt accgccgctt agcaaaattc ctttcatttt acgtccgcag gcgtggctgc
    31321 atcgtcgcca ttacggcgag gtgctaagcc cgattcgctg gtgggggcgg atcccgttta
    31381 tcttttattt ggtgtcaatg tttgttgggt ggctggagcg caaacgctca ccgctcgatc
    31441 cggtagtgcg atcgcttgtc agcgcgcgca ttgcgcaaat gtgcctgtgt gagttttgcg
    31501 tggatatcac cagtatgaaa gtcgccgagc gtaccggcag caccgataaa ctgctggcag
    31561 tggctgactg gcggcaaagc ccgctcttta gcgatgaaga acggctggcg ctggagtacg
    31621 ccgaagccgc aagcgtcaca ccgccaacag tcgatgatgc cctgcgtacc cgactggctg
    31681 cgcattttga cgctcaggcg ctcaccgaac tgacggcatt gatcggcctg caaaacctgt
    31741 cggctcgttt taattctgcc atggacattc ccgctcaggg gctgtgccgt attccggaaa
    31801 aacgttctta aggagggaag atgcgccatt gtgggtggtt gctgggattg ttatcgctgt
    31861 tttctttggc aacacatgcc agtgactggc aggaaattaa aaatgaggcc aaagggcaaa
    31921 ccgtctggtt taacgcctgg ggcggcgata ccgcaattaa ccgctatctc gactgggtta
    31981 gcggcgagat gaaaacccat tacgctataa acctgaagat tgttcgtctc gcggatgccg
    32041 cagacgcggt gaagcgcatt cagaccgaag ctgctgccgg tcgtaaaacg ggcggctcgg
    32101 tggatctgct ctgggtgaac ggcgaaaact tccgcacctt aaa
//
LOCUS       DAEEUE010000040        32129 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.040, whole genome shotgun sequence.
ACCESSION   DAEEUE010000040 DAEEUE010000000
VERSION     DAEEUE010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32129
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.040"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="KLK84_001989"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="KLK84_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HBE2009320.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="KLK84_001990"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="KLK84_001990"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBE2009321.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="KLK84_001991"
     CDS             complement(3736..4092)
                     /locus_tag="KLK84_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBE2009322.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="KLK84_001992"
     CDS             complement(4096..4512)
                     /locus_tag="KLK84_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HBE2009323.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="KLK84_001993"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="KLK84_001993"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HBE2009324.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5738..5942
                     /locus_tag="KLK84_001994"
                     /pseudo
     CDS             5738..5942
                     /locus_tag="KLK84_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="KLK84_001995"
     CDS             complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="KLK84_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HBE2009325.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7891..8970)
                     /gene="alr"
                     /locus_tag="KLK84_001996"
     CDS             complement(7891..8970)
                     /gene="alr"
                     /locus_tag="KLK84_001996"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBE2009326.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="KLK84_001997"
     CDS             complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="KLK84_001997"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBE2009327.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10521..11504
                     /locus_tag="KLK84_001998"
     CDS             10521..11504
                     /locus_tag="KLK84_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HBE2009328.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="KLK84_001999"
     CDS             complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="KLK84_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HBE2009329.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="KLK84_002000"
     CDS             complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="KLK84_002000"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HBE2009330.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13446..14431)
                     /locus_tag="KLK84_002001"
                     /pseudo
     CDS             complement(13446..14431)
                     /locus_tag="KLK84_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14749..15264
                     /gene="zur"
                     /locus_tag="KLK84_002002"
     CDS             14749..15264
                     /gene="zur"
                     /locus_tag="KLK84_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HBE2009331.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15306..15515)
                     /locus_tag="KLK84_002003"
     CDS             complement(15306..15515)
                     /locus_tag="KLK84_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBE2009332.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="KLK84_002004"
     CDS             complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="KLK84_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HBE2009333.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="KLK84_002005"
     CDS             complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="KLK84_002005"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HBE2009334.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="KLK84_002006"
     CDS             complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="KLK84_002006"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBE2009335.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18286..20709
                     /gene="plsB"
                     /locus_tag="KLK84_002007"
     CDS             18286..20709
                     /gene="plsB"
                     /locus_tag="KLK84_002007"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HBE2009336.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="KLK84_002008"
     CDS             complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="KLK84_002008"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HBE2009337.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="KLK84_002009"
     CDS             complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="KLK84_002009"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HBE2009338.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22469..24049)
                     /locus_tag="KLK84_002010"
                     /pseudo
     CDS             complement(22469..24049)
                     /locus_tag="KLK84_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24278..25198)
                     /gene="malM"
                     /locus_tag="KLK84_002011"
                     /pseudo
     CDS             complement(24278..25198)
                     /gene="malM"
                     /locus_tag="KLK84_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
     gene            complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="KLK84_002012"
     CDS             complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="KLK84_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HBE2009339.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26762..27877)
                     /gene="malK"
                     /locus_tag="KLK84_002013"
     CDS             complement(26762..27877)
                     /gene="malK"
                     /locus_tag="KLK84_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HBE2009340.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28242..29432
                     /gene="malE"
                     /locus_tag="KLK84_002014"
     CDS             28242..29432
                     /gene="malE"
                     /locus_tag="KLK84_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HBE2009341.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29586..31130
                     /gene="malF"
                     /locus_tag="KLK84_002015"
     CDS             29586..31130
                     /gene="malF"
                     /locus_tag="KLK84_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HBE2009342.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYVWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31145..32035
                     /gene="malG"
                     /locus_tag="KLK84_002016"
     CDS             31145..32035
                     /gene="malG"
                     /locus_tag="KLK84_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HBE2009343.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
BASE COUNT         8172 a         8246 c         7782 g         7929 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gttgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaatccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcaaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctttttat acatagaaac acagttaaaa attgcaaaag atttttgtag
     5881 acctggagaa gaagtatcaa gttctattgc aagtaaagtt tttcacgcat ttattgattt
     5941 agttaacaaa atcaggggta agaaagactt tatgtatatt tgcacgcttt gctgttttgc
     6001 cgaggaggtt aaaggtgatt attctcatta caggaccttt ttatttgata ttggtaatca
     6061 atataaggtt aaacttacac aaagcggaaa aaagagttct ctttaacatt agaatttaac
     6121 gatactataa ttgaatctca gaaagtcaca ggcaataagg caaagcatat tcttgaggat
     6181 atagaaatat tctatcgtaa caaacccgat acttattatt aaatttaata atggttagat
     6241 gtgcagaaat tattactcca cacgaaaaaa tagggaaagg aattccagat aattgggata
     6301 aacttaacaa taaaagaact gacaacgttt tattatctct cacgtagaac aacggcatca
     6361 aaaaaatcat ctacaaagaa atagtgaaag cgacgacagt ttaatttcac tgcaggctgg
     6421 gtaactccag cctgctttcc tgcattacat caccgcagca aacgcctttg ccacacgttg
     6481 tacatttgcc gtatttaacc cggcgacaca catgcgaccg ctggcgatga gatagacacc
     6541 aaattcttca cgtagtcggt caacctgagc ggcacttaaa ccggtataac tgaacatgcc
     6601 gcgctgatta agcagataat cgaaattgcg ttctggcatc tctgtgctta ataccttcac
     6661 caattcctga cgcattgcca gaatgcgagt acgcatctct tctacttccg ccagccagct
     6721 ggctttcaat gcctcgtcat tcagcactgc agccaccacc tgcgcaccaa aattcggcgg
     6781 gctggagtag ttgcggcgaa ctgttgcttt caattgcccc agtacgcggc cagcggcttc
     6841 ggcatcttca cacagaacag aaagtccgcc gacgcgctcg ccgtaaaggg agaaaatttt
     6901 cgagaacgaa ttgctcacca gagcgggtaa tccagcgctg gcaatggcgc gaatagcgta
     6961 ggcatcctct tccataccgg cactaaatcc ttgataggca atatcgagga atgggataag
     7021 ctcgcgggct ttgagaattt caatcaccgc atcccactga tcattagtga gatcggcacc
     7081 cgttgggttg tggcaacatg gatgcagcaa cacaatactg cgggcaggta atgttttcag
     7141 cgtcgccaac aggtcattaa agcgcacgct gttagtcact tcgtcatacc aggggtaagt
     7201 actcacttcg aatccagccc cggcgaatat tgctacgtgg ttttcccaag taggatcgct
     7261 gacccagacg cctgattccg ggaagtagcg tttcaggaaa tccgcgccca ctttcaatgc
     7321 ccctgagccg ccaagggttt gaatggttgc tacgcgctgt tgttgcagta ccggatggtc
     7381 cgcaccaaac agcagcggcg caatggcatg gcgatagctg ttaagccctt ccatcggtaa
     7441 ataaagcgaa gcgccatggg gctgcgcatt caggcgcgct tccgcctccg ccacggcttt
     7501 caattgtgga ataattccgt cttcgttgta gtacagaccg atacttaaat tcactttgtc
     7561 gctgcgaggg tcttctttaa aacgctccat aagcgtaaga atcgggtcgc cagcgtaggc
     7621 gtcaactttt tgaaacacgc gatggttctc caggtttacg ggcaggtggt taaaacacaa
     7681 taaaccggaa gaaggcgaag atcgagtgga tgttcaggag cgaacggcaa ttagcaacag
     7741 agtgagacta atgataaacg tacatccgcc agaggcacga cctttataag agcatgaaag
     7801 aacatcaact tattgaattt ttaggatttt attggccgga taaggcattc acgccgcatc
     7861 cggcacagac aatcaaatat tacagaacga ttaatccacg tatttcatcg cgacccttga
     7921 agtcaggcgc gtaataagtt cgtaagcgct tactttcgtc atttcagcga tacgttctac
     7981 gggcaaacct tcgccccata aaatgaccgg atccccggct ttgtcctgcg cctgtggacc
     8041 taagtctacg cagatcatat ccatcgccac gcgcccgaca atcggtactt cgcgaccgtt
     8101 caccagcact ggcgtaccgg acggcgcggc gcgcggataa ccatcgccat agcccatcgc
     8161 gactacgcca agacgggtat cacgttcgct tacccaggtt ccaccataac caacaggctc
     8221 tccggcttta tgctcacgca cggcaatcag gctggaggtt agtgacatca ctggctgaca
     8281 gccaaaatcg gcaccggtgg agcgatcttc cagcggcgag acgccataaa gaatgatgcc
     8341 cgggcgcacc cagtcaaaat gcgactgtgg ccacagcaga atgccacccg acgcggcaat
     8401 ggaacgttga ccaggtttgc cttcgcaaaa ggtattaaag atagcgagtt gtttctcggt
     8461 tgcgccacat tttggttcat ccgcgcgcgc aaaatggctg acgatattca ccggctgacg
     8521 aacgtttttg cactgggtca ggcgatgata aaacgcctca gcctgttccg gccttacgcc
     8581 cagacggtgc ataccggtat cgagtttcat ccagacggta accggctcgt ccaagctagc
     8641 ctcttccagc gcagccagct gttcttcgtt atgcacggcg gtatgaaaat gttgcgcaga
     8701 aatcgtcggc agatctctgg catcaaaaaa gccttcgagt aacagtacag gtttggtgat
     8761 tccccccgca cgcagtcgca gagcttcttc gagacgggct acgccaaagg cgtcagcatc
     8821 ggggagcgtt cgcgcggtct caagaagacc gtgaccataa gcgttcgctt tcaccaccgc
     8881 aaccatttta ctggcaggtg ccagttcacg aagacgttgc aggttgtgtc gcagagcgcg
     8941 gcggttaatc acaacagttg ccgcttgcat ttgtgttcct tgataattgt ttgctttaat
     9001 tacctaattc ataaaataat tattattcgt cgtcgtactg cggccccgca tagttgtcga
     9061 agcgcgacca ttgaccgtta aaggtcaggc gtaccgtccc gattgggccg ttacgttgtt
     9121 taccgataat aatttccgcg atgcctttta aatcactgtt ttcgtgatac acctcatcac
     9181 gatagataaa catgatcaag tccgcatcct gctcgataga gccagattca cgcaggtcgg
     9241 agttgaccgg acgtttgtcg gcacgttgtt ccagagaacg gttcaactgg gacagcgcca
     9301 ccaccggcac gttcagttct ttcgccagtg ctttcagcga gcgggagatt tctgcaattt
     9361 ccagcgtacg gttatcggaa agcgccggta cgcgcatcag ttgcaggtag tcgatcatga
     9421 taagcccgat gccgccgtgt tcacgggcaa tacggcgtgc gcgggaacgc acttccgttg
     9481 gcgtcaggcc ggaagagtca tcgatataga tattgcgttt ttcgagcaaa atacccatgg
     9541 tgccggaaat gcgcgcccag tcttcatcat cgagctgccc ggtacggatt ttagtctggt
     9601 caacgcgcga cagcgacgcc agagaacgca tcatgatctg ttctgatggc atctccagcg
     9661 agaagataag tactggttta tcctgcaaca ttgccgcgtt ttcgacgagg ttcatcgcaa
     9721 atgttgtttt acccatcgac ggacgcgcgg cgacgatgat caaatccgac ggctgcaagc
     9781 cagcggtttt tttgttgaga tcgtcataac cggtgtttac cccggtaacg ccatcgtgtg
     9841 gctgctgaaa caactgctca atacgcgcca cggttgcgtc gagcacatcg gcgatgttct
     9901 tcggcccttc gtctttgttt gcacgacttt cggcaatttt aaagacgcgg gattcagcaa
     9961 ggtccagcag atcttcgctg gtacgcccct gcggatcaaa accggcttca gcaatctcat
    10021 tcgcaaccga gatcatctca cggacaacgg cacgttcacg cacgatgtcc gcataagcac
    10081 tgatgttcgc cgcacttggc gtattttttg acagctctgc cagataagca aaaccaccga
    10141 cgctatcgag ttgcccctgg cgttccaacg attccgcaag ggtaatcaga tcgataggac
    10201 taccgctttc ctgcaaacgc gccatttcag taaagatatg acggtgtggg cgggtgtaaa
    10261 aatcgtctgc taccacacgc tcggctacat catcccagcg ttcgttatcc agcattaaac
    10321 cgcccaacac cgactgctcc gcttcgatcg agtgcggagg cactttcagc ccggcaactt
    10381 gtggatcgcg ttcgcggggt tcagcctgct gtttgttgaa gggtttattt cctgccatag
    10441 tgaatggagt taccgaaata aagaatgggt cgaaacttta ccatatgaag cagaccctga
    10501 cgatacgttc tggaggacac atggcaacac gaattgaatt tcacaagcac ggtggcccgg
    10561 aagtacttca agccgtagag ttcactcctg ccgatccggc ggagaatgaa atccaggtcg
    10621 aaaataaagc catcggcatc aattttatcg acacgtatat ccgcagcggc ctttacccgc
    10681 cgccatcgct acccagcgga ttaggtaccg aagcggcagg catcgtgagt aaagtcggca
    10741 gtggtgtaaa gcatattaag gctggcgatc atgtagtcta tgcgcagtcg gcgttaggcg
    10801 cttacagctc tgtgcataac attaatgcgg ataaagcggc gattctgcct gcggcaattt
    10861 cttttgagca agctgcggca tccttcctga aaggcttaac ggtttattat ctgctgcgca
    10921 aaacctatga aattaaaccc gatgaacagt tcctgttcca cgcagcggct ggcggcattg
    10981 gcttaattgc ctgccagtgg gcaaaagccc tgggcgcgaa actcatcggc accgtaggaa
    11041 ccgcgcaaaa agcgcagagc gcgctaaaag ccggcgcgtg gcaggttatt aactatcgtg
    11101 aagagaatct ggtcgagcga ttgaaagaga tcaccggcgg taagaaagtg cgcgtagtgt
    11161 acgattccgt gggcagagac acctgggaac ggtcgctgga ttgcctgcaa cgccgcggct
    11221 taatggtcag ttttggcaac tcatcaggtg cggttaccgg tgtgaactta ggcattctca
    11281 atcaaaaagg ctcgttgtat gtgacacgcc cttccctgca aggctatatc accacgcggg
    11341 aggaattaac cgaggccagt aatgaactgt tctctttgat tgccagcggt gtgattaagg
    11401 tcgatgtcgc cgagcagcag aaatatccgc tgaaggatgc gcagcgtgcg catgagattc
    11461 tggaaagccg ggcgacgcaa ggttccagcc tgctgattcc ataaaagaaa tagggcttcc
    11521 acctgggaag ccctttcttt ttatagttcg gctgtatgta gggtacagca cgatgaatct
    11581 gttagaggcg caatagtgac agatttgatt atcaattcct attttgttct aaggataaaa
    11641 ccttaggttg tgatcatccg cacaatccct tagtaacgcc agcggtcata acgctgatat
    11701 ttcggcattt ttggtgcttt aatcgcctta ataacccaca ccaccgcaat cgccagtagt
    11761 aaccacggca gcaacttaat catcaatgcc agcataccgc cgaggaacat aatggccgtc
    11821 gccacaacca gcgcggcgat aatgcccagc aacgaaacgc cggtgaccat cagcatgaca
    11881 aaaaagccaa tcacaaaaag tagttccagc atgatgctct cccaaatatg aaatctcttg
    11941 ctggcattac aagaatcatg ccaaaaataa tctattgatt taacagcaaa acgccccgcg
    12001 acggtgcgca gggcgtggtg aatttgacta ctttttggtg aaaagttaac gcttatccgc
    12061 caccagtttg agcgcgtgtt ccagcacgtt aatgtcagca cccgctttat gggcattttc
    12121 acttaaataa cgccgccact gccgcgcgcc aggaataccc tggaacaaac ccagcatatg
    12181 ccggataata tggccgagat acgtcccctg gctgagttca tgctcaatgt acggatacat
    12241 ggcacgcact actgccaccg gatcggcatc ggtatccgag gaaccaaaga tctcccggtc
    12301 taccgccgcc agaatacccg gattctgata cgcctcgcgc ccgaccatca cgccatccat
    12361 atgttgcaaa tgcgctttag cttcttccag cgacttgata ccaccgttaa tcgacattgt
    12421 cagatgcggg aagtcacgct tcagttgata cacacgcgga taatcgagcg gcgggatttc
    12481 acggttttct ttcgggctta accccgaaag ccaggcttta cgtgcgtgga tgatgaacat
    12541 ctcacactcg cctttgccgg aaacggtatt gatgaaatcg cagagaaatt catagctgtc
    12601 ctggtcgtcg atgccaatac gcgttttcac cgtcaccgga atcgacacca catcgcgcat
    12661 cgctttcacg cagtcggcaa ccagctgcgc attacccatc agacacgcac caaacatgcc
    12721 gttctgcacc cggtcagacg ggcagccgac attcaggttg atctcatcat atccgcgcgc
    12781 ttctgccagc tttgcacact gcgccagcgc cgccggatcg ctaccgccca gttgcaacgc
    12841 taccggatgt tcttcttcac tgtacgccag ataatcacct ttaccgtgaa taatcgcccc
    12901 tgtggtcacc atttcggtat aaagcaacgt attgcgggaa agcagacgca agaaatagcg
    12961 gcaatgtctg tccgtccagt cgagcattgg tgcaacacta aaccgaccac tccagtgaac
    13021 gtcagttttt tcaggcattg cgctggtttg gttgattttt tgcatttcag aattaccgtg
    13081 cattttcaaa tgtagagatt attttatcga tatatcatgg ggttatgtta ttcagcttca
    13141 ctgttcagga ggctcaatag cggggtacta taccataaca acaggaagcg cctgtctcat
    13201 tgcaaaagaa aattgagatc ctctcaaggc atgaagctct cacgaagtga tggaaataat
    13261 cttattagcc gttaactttg ttaaggccaa tgataaacaa tccaggttcg acgataaata
    13321 aaaaatcaca cattaaactc tggtgatata tctccctgct aatagcattg atagagaaaa
    13381 aagaacccaa taaatattgg gtccttttat ataatgcctt ccatactatc gaagaacttc
    13441 acatattatt tctccgattt aaccccgaac aagtcataaa ttaatttaga agtgtctgta
    13501 agtattttaa tctcttcctt tgaggttggg tcaaacgact tcgcgaagct aattaattgt
    13561 ggtgcagcat ctcgcatttt gcttaaaata tcaggatcga gcgttccttc attcaccaga
    13621 tgtgacatct tatccagata gccatcaaaa ttcatttctc ccccatctgc cagacgcttc
    13681 atctctccca ggtacttctt catatcacgt tgatataatt tttcaaactg agctttcagg
    13741 tagctctcat tattttcatt aatatacact gtttctgata aatctgcgta ggcactagaa
    13801 tatgaaagtg aaattaacag cgaagcaaat aattttaagc tgttgtcatc atgttcctgg
    13861 caggcattaa caaacgttaa aaaaccagaa cctattgctt gaaaatgtat attcgctaaa
    13921 ggttcattat ccttagattc ctcataaaat agctgaaccg tggagggaag gccattctta
    13981 gtttctacat caaaagtaca ttgttcaatg ggcattgcat ctttattatc cccaggatta
    14041 catatactat taatcgcgaa atgaaaaata gcccggtcaa caactgatgc aataaagtca
    14101 cgatcgttta aatcctcatc agtgcaatcg tcagtatact tattgaccca cattttgaga
    14161 tcatttatgt gctcaattaa aaacacattt ttttacgtgc atgccataaa ttatgcgtga
    14221 ttcgttgaat aaacaaatct atttttgcaa taattatatc ccgatgacgc agcgctgcat
    14281 aatcaataga tttaccatat atattattta atttaccttt gattgaacct ctattatcca
    14341 atttatcttt ggttgcatct ttattttcga attgatttga attgagtgtt ttattaaagt
    14401 atccagttgc accgggtatt atcttcaaca tgattcatct accttttttg tcgtaccgtg
    14461 tcctccttat aattcttttg ccatctggtc actatcaaaa atcgctcata atttctgaac
    14521 tacaggtatg cgtttaaacc cagttaaata aatagcgagt taacttgaag caatttattt
    14581 aaaaatgaat tattaattat tatcgtgttg ataatggtac gacaaggcaa cacaacacct
    14641 tacctacaaa acagaaaatc aacattacgg caacaataag ggttctcggt gtttttcctg
    14701 gaacatggta aagtaaggac attcttaacc cccactttga ggtgcccgat ggaaaagacc
    14761 acaacgcagg agttattagc gcaggctgaa aaaatctgcg cgcagcgtaa tgtgcgcctg
    14821 accccacagc gcctggaagt gttgcgcctg atgagtctgc aggatggcgc tatcagcgct
    14881 tatgatctgc ttgatttact gcgcgaagct gaaccgcaag ccaagccgcc aacggtttat
    14941 cgcgcgctgg attttctgct tgagcaaggt tttgtgcata aggtggaatc caccaacagt
    15001 tatgtgctct gtcatctgtt cgaccagccc acgcatacgt cagccatgtt tatttgcgat
    15061 cgctgcggcg cggtgaaaga agagtgtgca gaaggcgtgg aagacattat gcatacgctg
    15121 gcggcaaaaa tggggtttgc cctgcggcat aatgtgattg aagcacatgg gctctgtgcg
    15181 gcatgcgtgg aagtggaagc gtgtcgtcat cctgaacagt gccagcatga tcactctgtg
    15241 caggtgaaaa agaaaccgcg ttaagagggc gtacatcctt gtacacgtcg ggcaggaggg
    15301 attaattacc agcgatattc attgcgggtt tcccaatcca cgacctcttt ttctgcctga
    15361 tctttctgat aaccataacg ttcctggatt ttaccgacca gttgatcacg tttaccttca
    15421 atgatcgtca tatcatcatc ggtcagtttg ccccattgct ctttcacttt acctttaaac
    15481 tgtttccagt taccgccggc ttcatcttta ttcataatca agacctcatc gttaggttgt
    15541 gaatgagagt acgttcactt ttcttctgaa cgtgagatta agtgtagtca gcaatttggc
    15601 ttaggataat ttattcagaa tttttaaccg tcacgttgcg gcaaaccagg taccgttgcg
    15661 ccagtgacgc cgccagatag ccgccagaga aagcccgcgc aacgccagaa agacggttaa
    15721 tgccagccac aaaccatgat tacccagcca cggcagcgta aggagcgtca gcgcaaaacc
    15781 tgcggcggcc accgccatac tgttacgcat ttcggcggca cgcgttgcgc ctataaacat
    15841 gccgtccagc agataacacc agacgccaac caacggcaaa atcacctgcc agataagata
    15901 gcggtcagcc agctgctgaa tctgggttaa cgacgtcagc aacgcaatga tgtgttcccc
    15961 tgccagcaaa taaaccaccg aaaacagtaa cgctacaatc cccgactggc ggcacgctgc
    16021 ccgccataca tccagcaact ggctaccgtc gcgcgcaccg tatgcctgac cggagtgcgc
    16081 ttcaaccgcg taggcaaaac catcaagcgc ataggcggta aaggtgagta gcgtcatcag
    16141 aaccgcgtta acagcgataa tgtcactccc cagtcgcgca ccaagtacgg tgatcgcgcc
    16201 gaaacagagt tgcaacaaca gcgaacgcag catgatatcg cggttaagcg ccagcaagcg
    16261 acggaagttt cctcgccagg cagttttcag catttcgccg gaaattccgc gtagtttgag
    16321 gattttacgc accattagca gaccaatcag caatgttgca tattccgcaa taaccgtcgc
    16381 cagcgccgcg ccctgcacgt tcatatgcag ccccatcacc agccagacat ccagcacaat
    16441 gttgaggata ttgccgacca ctaacaaaat tactggcgca cgggcatatt gcacgccgag
    16501 taaccaaccg agtaatacca gattcgccag cgacgccgga gcgcttaacc agcggatttc
    16561 aagaaagcgc cgcgcctgtt ccaggaccgc ttcactaccg ccaacaatat gcagcgccag
    16621 atcgataatc ggcgtacgca acagcgcaat taacgccccg gcccccaacg ccaacagcaa
    16681 cggttgcacc agcgcacggg ctaatgcctg aggatttttg gcaccataag cctgcgcagt
    16741 cagcccggtg gtgctcatgc gtaaaaacag caacagcata aagagaaagc tggtcgccgt
    16801 tgcaccaacc gccacgccgc ccaaataaac cggactatca agatgaccaa ttaccgccgt
    16861 atcgaccagt cccagcaacg gaacggtgat attggagaaa atcatgggta aggcgagatg
    16921 ccagagtgct ttatcagatg aagtgaggaa tgccatgcag acaagcctga tgaagagaga
    16981 tgaaaaacaa accgcgacgc caagcggcat cgcggtctca gagatatgtt acagccagtc
    17041 gccgttgcga ataaccccaa ccgccagccc ttcaatggtg aagctctgct gacgaaggtc
    17101 aacgacaatt ggtttaaact cgctattttc tggcaacagt tcgactttat tgccctgttt
    17161 tttcaggcgc ttaacggtaa cttcgtcatc aatacgtgcg acaacgacct gaccgttacg
    17221 tacatcctga gttttatgca ctgccagcaa gtcgccatcc ataatgccga tatctttcat
    17281 cgacatcccg ctgacgcgca gcaggaaatc agcattcggc ttgaacaagg aaggatcgac
    17341 ctgataatga ccttcaatat gctgttgcgc cagaagcggt tcaccggcag ccacacgacc
    17401 taccagcggc aacccttctt cctcttcctg caacagacga atcccgcgtg atgcgccgga
    17461 aacaatttca ataacgcctt tgcgtgccag cgccttcaga tgttcttcag ccgcgtttgg
    17521 ggaacggaac cccaaacgct gcgcgatttc cgcacgcgtc ggcggcatac ctgtctggct
    17581 gatgtgatca cggatgagat caaacacctc ttgttgcctg gccgttaacg ctttcattcc
    17641 gccccctggg tgtatataca gttatactgt gagtatatac agcaaaaggc gattttggaa
    17701 ccataaactg cacaataaac cagagattta tcgaaatctg gaagggttat ccaaaatgcg
    17761 accataacag gatgcaccag gtaatcacgg cgacgataat ggcaatcagc accgcggcgg
    17821 atcccatatc ttttgcgcgt ccggaaagct catggtattc agagccaatt cggtcaacca
    17881 ctgcttcgat ggcgctattg aggatttcca caatcatcac cagcatcacg gagctgataa
    17941 gcagcacgcg ggtaattgcg tccacatcca gccagcaggc gatgaccacc gccaacaata
    18001 ccgctacgcc ttcctgacgg aatgccgctt cgttgatcca tgcagcgcgt aaacctttcc
    18061 aggaatagcc agcagctttg ataattcggg tgaatccagt tgtattattg gccattgaaa
    18121 gaaccttttt acattatgag cgtcaatatc agtgtaccgt taaatccgat gattcgggtt
    18181 tgcgtaacgc cgcgcgaaac atgagcggat accacagaat ttcccatgac tttctgctat
    18241 ccttgccgcg catttgcatt attaaccaga ggctttacat cgtttatgtc cggctggcca
    18301 cgaatttact acaaattact gaatttacca ttaagcatcc tggtaaaaag caagtctatt
    18361 ccggcagatc ctgccccgga actggggctg gatacctctc gtccaattat gtacgtttta
    18421 ccgtacaact cgaaagcaga tttgctgacg ttgcgcgccc agtgtctggc acatgatttg
    18481 cctgacccgt tagagccgct ggaaatcgac ggcacgctac tgccgcgcta tgtgttcatt
    18541 cacggcgggc cgcgtgtatt cacctattac acgccgaaag aagagtctat taagcttttc
    18601 cacgactatc tcgatttgca ccgtagcaac ccaaatctgg atgtgcagat ggtgccagta
    18661 tcggtgatgt ttggtcgcgc gccggggcgt gaaaaaggcg aagtgaaccc gccgctgcgt
    18721 atgcttaacg gcgtacagaa atttttcgct gtactgtggc tcggtcgcga cagttttgtg
    18781 cgtttctcgc cgtcagtgtc gctgcgccgt atggcggatg aacacggcac ggataaaact
    18841 atcgctcaga aactggcgcg cgtggcgcgt atgcactttg cccgtcaacg tctggctgcc
    18901 gtaggcccac gtctgcctgc tcgtcaggat ctgtttaata agctgctcgc ctcccgcgcc
    18961 attgccaaag cggtagaaga tgaagcgcgc agcaaaaaaa tctcccatga aaaagcgcag
    19021 cagaacgcga ttgcgctgat ggaagagatt gcggcgaatt tctcttacga gatgatccgc
    19081 ctgactgacc gcattctggg cttcacctgg aaccgatttt atcagggcat caacgtccat
    19141 aacgccgagc gcgttcgcca gctggcccac gacggtcatg agctggtgta tgtgccttgc
    19201 caccgcagtc acatggacta cctgctactt tcttacgtgc tgtaccacca ggggctggtg
    19261 ccgccgcata tcgccgccgg gatcaacctg aacttctggc cggccgggcc gattttccgc
    19321 cgtctggggg cgttctttat tcgccgtact tttaaaggca ataaacttta ttccaccgtt
    19381 ttccgtgagt atctcggcga actgttcagc cgtggttatt ccgtcgagta cttcgtggaa
    19441 ggcggtcgtt cccgcacggg gcgtttgctg gatccgaaaa ccggtacgct gtcgatgacc
    19501 attcaggcga tgctgcgtgg cggcacgcgt ccgattacgc tgattccgat ctatatcggt
    19561 tatgagcacg tcatggaagt gggtacttac gccaaagaac tgcgcggcgc gacgaaagag
    19621 aaagagagcc tgccgcagat gctgcgcggt ttaagcaagc tgcgtaatct cggtcagggt
    19681 tacgtcaact tcggtgaacc aatgccgttg atgacctacc ttaaccagca cgtaccagac
    19741 tggcgtgaat ctatcgatcc catcgaagcg gtgcgtccgg cctggttaac gccgacggtc
    19801 aataatattg ctgccgatct gatggtacgc attaacaacg caggcgcggc aaacgccatg
    19861 aacctgtgct gtactgcgct actggcatca cgtcagcgct cactcacccg cgagcagtta
    19921 accgagcaac tcaactgcta cctggatctg atgcgcaacg taccttactc cacggactct
    19981 accgttcctt cagccagcgc cagcgagctt atcgatcacg cgctgcaaat gaacaagttt
    20041 gaagtcgaga aagacactat cggcgacatc atcattctgc cacgcgagca agcggtgctg
    20101 atgacctact atcgcaacaa cattgcgcat atgttggtgc tgccttcgct gatggcggca
    20161 attgtcaccc agcatcgcca catctcccgc gacgtattga tggagcacgt caatgtgctt
    20221 tacccaatgc tgaaagcgga gctgttcctg cgctgggatc gcgacgagtt gccggacgtt
    20281 attgatgcgc tggcaaatga gatgcaacgt caggggctga ttaccctgca agatgatgag
    20341 ttgcatatca acccggcgca ttctcgcacc ctacagctgc tggccgcagg cgcgcgcgaa
    20401 acgctgcaac gttatgccat caccttctgg ttgttgagtg ccaacccgtc gatcaaccgc
    20461 ggtacgctgg agaaagagag ccgcaccgtt gcgcaacgtc tctccgtgct gcacggcatc
    20521 aacgcgccgg agttcttcga caaggcggtg ttcagttctc tggtgctgac gctgcgtgat
    20581 gaagggtata tcagcgatag cggcgatgcc gaaccagcag aaacgatgaa ggtttatcag
    20641 ttgctggcgg agttgattac atcagacgtg cgtttgacga ttgagagtgc gacgcagggc
    20701 gaagggtaat cagagagaat tgccggatgc ggcgaaaacg ccttatccgg cctaccatga
    20761 cctgcaaatt caataaattg cgattcaccg ggtaggcctg agaagcgcaa cgcatcaggc
    20821 aacccagaag aaagccggat gatcatccgg cttttttaca tcatcagaaa tgccagtaac
    20881 tcattgccag tcctaaaaac agtaccagac caacatagtt attattcata aatgctttaa
    20941 agcaggcttc acgctcgcgg ttggcaatca gtttttgttg ataaacaaac agcgcgccag
    21001 ccaccacaat tgaccagtaa tatccccagc ctaagccatt taactcaccg atgatcgcca
    21061 tcagtgccag tacgccaatc tgcaaaatac caataatcaa tttatcgtat tggccgaaca
    21121 ggattgccgt ggatttaatg ccaatcttca catcatcatc gcggtcaacc atcgcatact
    21181 gcgtgtcgta agccaccgcc cagagaatat tggcgaggaa cattaaccag caactcaatg
    21241 gcaccgactc actcacagcg gcaaaagcca ttggaatcga ccagccaaac gccgcgccca
    21301 gcaccacttg cgggagatgg gtataccgct tcataaacgg gtacacccac gccaatgcca
    21361 gagcggcaat cgacaacaga atggtcatcg tattcagcgt cagaaccagt aaaaacgaaa
    21421 tcagtaccag cactacaaac agcgcgcgcg cctctttctc tgttaccgcg ccgctgggaa
    21481 gtggtcggtt cgccgtgcgc ttaacatgac catcaaactt gcgatcagca taatcattca
    21541 ccacgcaacc agcagcgcgc atcagccaga cacccgcgac aaacaccgcc atgatccaga
    21601 gctgaggaac gcccggtgtc gccacccaca acgcccataa tgttggccag agcagcagta
    21661 acgcgccaat tggcttatcc gtacgcatta agcgatgaaa cgccagcagc ttattctgcg
    21721 tcagactcca ctccatattt ttttcctctt agtacaacgg tgacgccggt aaaaacagtt
    21781 ctgttagcaa cagcggttta ccgcttaatc gcaggcggga acgtcgcccc cacagtccgg
    21841 catcacggcc tatctcgata aaatcccggg ttagtgtcga tgatgtgaac agatagcgtc
    21901 ctaacggcgt tttacccaat ttttgtaacg ctagttccgg cccgcttaat gttgatacag
    21961 gaacgacggt acgaccggca agccacggtt caccatcggc acataacaaa atttcacgta
    22021 accagtaacg agactctttc ggcagcagcg gcagttcttc ggggatttca ttctgctcga
    22081 caaacccctc gcggatcatc gtcacactca ccgtttttcc ctgctgttca aaacgttttg
    22141 tcatggaatc ctccagcaac agccagtcga gcagttgcgg atccagggca gggatctctt
    22201 taaaatagcg cagcgcacgc agttgcgtta acgcggggtg tgacatgccg aactctccgt
    22261 tacataacgt aaaggcattg tatcgcagaa aagtgtttac gggagatgta aagcaaatta
    22321 agtgatctaa tgcgcaacaa aaacgcaaca atcgcatgaa gtggtaaaac gctgttgaac
    22381 cttacgcttt gccgatgccc gtgctggcga gggcaaccat aggggcatca ccgcccatga
    22441 tgctaggaac ggtgatgttt tcctttactt aaataccttt attctttata cgagtagctc
    22501 gccaactcgc agtaaaaatt gggaaaagcg catatttacc atgagatttg gagctgaaaa
    22561 gtgtttcctg agtaagatta ataggcaaca tcttatgtct ggatttatag atacacgtgg
    22621 aattccaatt ggtatctttt gtaggattca acatatcatg taagcgctgg gagcttacca
    22681 ccataacagg cccatcatcg acagaggaga acaggataaa tatattatcg tttcgtgtta
    22741 atttattagt gttaaaggtt cttaactttt cttcagtttc tataaatata ggtgaaacat
    22801 catcactacg aatccactct ttataaagat accaggcatt tgctttgatc atatcatccg
    22861 ttgatcgtaa ggcctgatcg ataagttgga taaaaaaatt gttattttta tcaaccagcc
    22921 ctttattcat attaaatctg ttaataaaat ggatcatctg ttgggtatcg aaattcacct
    22981 tactgaatcg atgttgacgc ataaaattat cgaacttgtc atcttcccca gaatgtatat
    23041 aactacgtat aattggatct tttatataag gacaatggct aaatgttttc ttcatattct
    23101 taataaagtt tttattaaca atattgagaa tttttaattg catcaccagt tttactgcca
    23161 gaacggattt caatatcatt gcattatctg gcaatgtatc aatcattttt ataatgttat
    23221 tgatataacc atcgagatct tcattcccaa atatatctga ataatcaata gttaattcca
    23281 attgttctgt aatgcaatct taaaaattcc ctgtcgacat tcgcacatga tcaaacataa
    23341 cagctgtcaa attcgattta ataaatgata tttctttggc agacagaaac tctgctctgg
    23401 tgaagtgcga agcgtaaaga taacaattat cgaagtttga atttctaatc tgacattcgc
    23461 gaaaattact atgaccaagt tgactctgcg agaagtcaac tgtatcaaga atattttggc
    23521 taaaatcaac acattcgcca ctcgttttga gaaaaatcac attgtgtaaa cgtcgacatt
    23581 taaatatgac attatcaact gttgccttag taaaattgca attctcaatt tctttacatt
    23641 cgaaagttac atgttgtaga tttgatgata aaaaagacac cttagaaata acgtcacctt
    23701 taaaatgaca atttgttaaa tcaaaatcag acaggtcgat atacccctgc gcatctttat
    23761 ccatcagaaa attacaagcg cattctattt tttcatttct gtagaatttt acacttatag
    23821 tatcattact aattgagact tcacgtaaca tatttattgc atgatttttt tcgttccgtt
    23881 gttttatatt taaaaatata tcactgatat aaataaagta atctctgttt aaacctgtca
    23941 ccccattgaa attacgtagt aaatcaagat ctactatatt atttccttgc accacatcat
    24001 gtttcatcaa agagttattg ccgccagaat ttgtgatatg acctatcata aaacctctgt
    24061 aatacagaaa taaaataacc tggaaaagta gcctgagcgt ccagacacta ccggcgaaag
    24121 gaaaagaatc tctgataatg cattgaaata atggacattc ttagtgaaga agaagtgaag
    24181 tactataaaa aatcgctcat atcccaggaa ggaagcataa aaaaaggtgc gccaggagac
    24241 gcaccagttg ttgcaaaatc agcactgtgg ggcgtaatta ccccttgcct tttacactgc
    24301 tgataaaggt ggaacgggca gatgtcgatc ccaggcgttc agcttcatca agcagtttta
    24361 acgccttatc aacatcacct ttcgcgacag cgtttttgat cgcggtatta aaataacttt
    24421 ccgtgtcgtt gagcatcggc tcgcttttct tcaccggcac cggagcaggt gcagccacag
    24481 ctggtgccgc cgtgttgcct accgtaaccg gagctggagc ggaagaacca aatagtggtc
    24541 ctaccaacac gctggagctg gagttcgttt tgactttcag tttcagtaag ccatcgctgg
    24601 tatgacgagc aaccggatcg ggaatatccg ggatcgagtt accgacgcct ttggcatagg
    24661 ctttagccgg gtcgagcagt tgggtcgtct gctggagatc tttttccgtg gtaaagacca
    24721 gaacataaag tttttgctgc cccaacgccg gtgtcaggcg cataacgcct tccagccgct
    24781 ctgcactcat cacgcctagt tcctggtagg tgaaataact gctggggaag aaggctgatg
    24841 gggtcatgtt ctgatcaaga atcagcacgt tcggcgcaaa aacgctggtt tgtttgttca
    24901 cttcgctggt cagcgtcagg ggcagttcgc caatgtttgc cgggacgctg tacgcagcaa
    24961 ccggaccact gatgccggga acgttcagtt gttggccgcc ggtcgccagt tgggtggtct
    25021 gggttttaga ttgatcgacc ggtgtctagg tgagttgttg cagcgcggca gatggaatgg
    25081 ctggcgcgtc gctggtgttt tgcggtacgt agttaacatc ggcaaggcta attccaggcg
    25141 cgcttgccag taaccctgct gataaacaga gggcgatgag acttttattc attttcattg
    25201 ttttcacctc aaaatctgga gctcagcggt agccaggcaa tagcgcgcta aacccgataa
    25261 tcagaggggc tttcgcccct ttcagataat gacaacctgt ttttatgccg gatgcggcgt
    25321 aaacgcctta tccggcccag gttttgctat taccaccaga tttccatctg ggcaccgaag
    25381 gtccactcgt cgctgtcgcc acgaccgaag ctgccgccgt tgaaatcagc aggaacggct
    25441 ttgccgtagt tcgggttaac cttgctatcg ccgttgtagt cgtaacccca tttctcatcc
    25501 cacttggcgt aggttgcgaa gacacgaata gccgggcgtg accagatgct gtcgccagcc
    25561 tgccattgtt gtgcgagggt aattttgtac tgattgttct tgtcgccggt gcgctgggat
    25621 tcgacgttgt cgtagccgat ttccatcacg gtgctcatga ttggcgtcca cttgtacatc
    25681 gggcgaatac caacggtcca ccacttggtg ccgttgtcgt tatcccagtt gatatcctgg
    25741 tacataccca cgtacatcat gtcccagttg tcgcccatgg agatcgcacc gtggtcgagg
    25801 atacgcagca tgtgaccgtt gttgttgata ttgtaggcaa atttttcgtt atcaaacgcg
    25861 acgccagaac cctgcgacag acctttaccc tgcgaggtca tcgagtcagt agcgtactga
    25921 acaacaaact tgttaaagcc cttcaggaca ctctgagtat gttcagcagt gaataaccag
    25981 ccgtctttcg atgcgccatc aaccagacga tagttatcac gcaggttggc acgaccgtag
    26041 tcgacaccca gttctaatgt gccgcccggg ttgatttcca tctgcgctaa acgcacatcg
    26101 aaaacgtcgt tcgcggtttc gttggtatag tcataaatat tgttgctggc aaaagaggaa
    26161 gaaccaccag cttcagagga gcgggttgct gccagagaga gtttaccgaa gccaacatcg
    26221 atgttttcca gaccggcacc aggaccagaa atatcccagt agtagaagtc gatcatatga
    26281 acgtcatgac gttggtagaa gcgcttacct gcccagatgg tggaacctgg cagccattcg
    26341 atcaggtttt ttccctgcac gtttgcttca cggaaggccg gatcggtagc ttcccagtca
    26401 ttctgttgcg cgacggaata ggccacgtta gtgtcgaaat agaagctctt atcgccctct
    26461 ttccacactt cctgacccaa ttttaattca gcataagttt cacattcgtt gccaagacgg
    26521 tatttacttt gagcaccggt agtctggaaa cactgttgtt caccgccgct acctgtccag
    26581 ccaataccgg aacgtgcata gccgtggaaa tcaacagcca ttgcctgagc agacattacg
    26641 cccgctgcga cggcaaccgc cagaggaagt ttgcgcagag taatcatcat tctatctcct
    26701 gagtcattgc ttttcttttt tcacatcacc tgtgacaggc tttgtgtgtt ttgtggggtg
    26761 cttaaacgcc cggctcctta tgcagtcgac gacatgcagt gccatcctca cggaacagat
    26821 ggcaacgctc tggcggcagg ccgatagcga atgtggcacc ttcttctacc aacaccacgt
    26881 cgttctggcg gtacaccagg ttttgacgaa tggaagggat ctggatatag atttgggttt
    26941 cgttgccgag ttgctcgacg acctgaactt caccctcaag gatgacgtca gcgatatcac
    27001 tcggcagtag atgttccggg cgaataccca gcgacatatt ggctccaacc tggacatcac
    27061 ggctttcaac aggcagccag acttgttgac gatttggcat cggcagctcc acctgcactt
    27121 gatcgattgc ggtggcggtc acttttaccg gcaggaagtt catctttggc gaaccgataa
    27181 atccggcgac aaaacggtct gccggatagt ggtacagttc cagcggtttc ccaacctgtg
    27241 ccacgcgacc ggcgtccagc accacgattt tgtcggccag cgtcatcgct tcgacctgat
    27301 cgtgggtgac gtaaatcatt gtgcggccca ggcgtttatg cagacgggag atttcgatac
    27361 gcatttgcac acgcagtgca gcatcgaggt tggagagcgg ttcatcgagc aaaaatacgc
    27421 ttggctcggc caccagcgta cggccaatcg ccacgcgctg acgctgacca ccggagagcg
    27481 ctttcggttt gcgatccagc aaatgcgcca gttgcagcac ttccgccacc tggttgacgc
    27541 gttggttaat cacctctttt tttgcgccag ccagtttcag gccaaatgac atgttttctg
    27601 ctactgacag gtggggatag agcgcgtaag actgaaacac cataccaacg ccgcgttctg
    27661 ctggcggagt gtcattcatc cgtttctcac cgatgaacag gtcgccgctg gtgatcgtct
    27721 caagcccggc aatcatgcgc agtaaagtcg atttaccgca gccagacggt ccgacaaaca
    27781 ccacgaattc accttcatgg atatcgagat tgatatcttt cgataccacg acctcgcccc
    27841 aggctttcgt tacattttgc agctgtacgc tcgccatgcc cttctctctt tgtaacaacc
    27901 tgtcatcgac agcaacattc atgatgggct gactatgcgt catcaggaga tggcttaaat
    27961 cctccacccc ctggcttttt tatgggggag gaggcgggag gatgagaacg cggcttctgt
    28021 gaactaaacc gaggtcatgt aaggaatttc gtgatgttgc ttgcaaaaat cgtggcgatt
    28081 ttatgtgcgc atccccacat taccgccaat tctgtaacag agatcacaca aagcaacggt
    28141 ggggcgtagg ggcaaggagg atggaaagag gttgccgtat aaagaaacta gagtccgttt
    28201 aggtgttttc acgagcactt caccaacaag gaccatagat tatgaaaata aaaacaggtg
    28261 cacgcatcct cgcattatcc gcattaacga cgatgatgtt ttccgcctcg gctctcgcca
    28321 aaatcgaaga aggtaaactg gtaatctgga ttaacggcga taaaggctat aacggtctcg
    28381 ctgaagtcgg taagaaattc gagaaagata ccggaattaa agtcaccgtt gagcatccgg
    28441 ataaactgga agagaaattc cctcaggttg cggcaactgg cgatggccct gacattatct
    28501 tctgggcgca cgaccgcttt ggtggctacg ctcaatctgg cctgttggct gaaatcaccc
    28561 cggacaaagc gttccaggac aagctgtatc cgtttacatg ggatgccgta cgttacaacg
    28621 gcaagctgat tgcttaccca atcgctgttg aagcgttatc gctgatttat aacaaagacc
    28681 tgctgccgaa cccgccaaaa acctgggaag agatcccggc gctggataaa gaactgaaag
    28741 cgaaaggtaa gagcgcgctg atgttcaacc tgcaagaacc gtacttcacc tggccgctga
    28801 ttgctgctga cgggggttat gcgttcaagt atgaaaacgg caagtacgac attaaagacg
    28861 tgggcgtgga taacgctggc gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa
    28921 acaaacacat gaatgcagac accgattact ccatcgcaga agctgccttt aataaaggcg
    28981 aaacagcgat gaccatcaac ggcccgtggg catggtccaa catcgacacc agcaaagtga
    29041 attatggtgt aacggtactg ccgaccttca agggtcaacc atctaaaccg ttcgttggcg
    29101 tgctgagcac cggtattaac gccgccagtc cgaacaaaga gctggcgaaa gagttcctcg
    29161 aaaactatct gctgactgat gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg
    29221 ccgtagcgct gaagtcttac gaggaagagt tggcgaaaga tccacgtatt gccgccacca
    29281 tggaaaacgc ccagaaaggt gaaatcatgc cgaacatccc gcagatgtcc gctttctggt
    29341 atgccgtacg tactgcggtg atcaacgccg ccagcggtcg tcagactgtc gatgaagccc
    29401 tgaaagacgc gcagactcgt atcaccaagt aatgctgtga aatgccggat gcggcgtgaa
    29461 cgccttgtcc ggcctacaaa actgaaacgc atgtaggcct gataagacgc atcagtgtcg
    29521 catcaggcag ttgttgtcgg ataaggcgtg aaagccttat ccgtcctgga atgaggaaga
    29581 accccatgga tgtcattaaa aagaaacatt ggtggcaaag cgacgcgctg aaatggtcag
    29641 tgctaggtct gctcggcctg ctggtgggtt accttgttgt tttaatgtac gcacaagggg
    29701 aatacctgtt cgccattacc acgctgatat tgagttcagc ggggctgtat attttcgcca
    29761 atcgtaaagc ctacgtctgg cgctatgttt acccgggaat ggctggaatg ggattattcg
    29821 tcctcttccc tctggtctgc accatcgcca ttgccttcac caactacagc agcactaacc
    29881 agctgacttt tgaacgtgcg caggaagtgt tgttagatcg ctcctggcaa gcaggcaaaa
    29941 cctataactt tggtctttac ccggcgggcg atgagtggca actggcgctc agcgacggcg
    30001 aaaccggcaa aaattacctc tccgacgcgt ttaaatttgg cggcgagcaa aaactgcaac
    30061 tgaaagaaac gaccgcccag cccgaaggcg aacgcgcgaa tctgcgcgtg attacccaga
    30121 atcgtcaggc gctgagtgac attaccgcca ttctgccgga tggcaacaaa gtgatgatga
    30181 gctccctgcg ccagttttct ggcacgcagc cgctctacac actcgacggt gacggcacgt
    30241 tgacgaataa tcagagtggc gtgaaatatc gtccgaataa ccaaattggc ttttaccagt
    30301 ccattaccgc cgacggcaac tggggggatg aaaagctaag ccccggttac accgtgacca
    30361 ccggctggaa aaactttacc cgcgtcttta ccgacgaagg cattcagaaa ccgttcctcg
    30421 ccattttcgt ctggaccgtg gtgttctcgc tgatcactgt ctttttaacg gtggcggtcg
    30481 gcatggttct ggcatgtctg gtgcagtggg aagcgttgcg cggcaaagcg gtctatcgcg
    30541 tcctgctgat tctgccctac gcggtgccat cgttcatttc aatcttgatt ttcaaaggat
    30601 tgtttaacca gagcttcggt gaaatcaaca tgatgttgag cgcgctgttt ggcgtgaagc
    30661 ccgcctggtt cagcgatccg accaccgctc gcacgatgct gattatcgtc aatacctggc
    30721 tgggttatcc gtacatgatg atcctctgca tgggcttgct gaaagcgatt ccggacgatt
    30781 tgtatgaagc ctcagcaatg gatggcgcag gtccgttcca gaacttcttt aagattacgc
    30841 tgccgctgct gattaaaccg ctgacgccgc tgatgatcgc cagcttcgcc tttaacttta
    30901 acaacttcgt gctgattcag ctgttaacca acggcggccc ggatcgtctt ggcacgacca
    30961 cgccagccgg ttataccgac ctgcttgtta actacaccta ccgtatcgct tttgaaggcg
    31021 gcgggggtca ggacttcggt ctggcggcag caattgccac gctgatcttc ctgctggtgg
    31081 gtgcgctggc gatagtgaac ctgaaagcca cgcgaatgaa gtttgattaa gggagataac
    31141 aaaaatggca atggtccaac cgaaatcgca aaaagcacgt ttgtttatta ctcacctgct
    31201 actgctactt tttatcgcgg cgattatgtt cccgctgctg atggtcgtcg ctatctcgct
    31261 gcgtcaggga aacttcgcga ccggtagcct gatcccggag caaatctcct gggatcactg
    31321 gaaactggcg ttaggtttta gcgttgaaca ggctgatggt cgcattacgc caccgccatt
    31381 cccggtactg ctgtggctgt ggaactcggt aaaggtcgct gggatttccg cgattggcat
    31441 tgtggcgctc tccaccacct gcgcctacgc tttcgcccgt atgcgctttc caggcaaagc
    31501 gacgctgctg aaaggaatgc tgattttcca gatgttcccg gcagtacttt cactggtcgc
    31561 gttgtatgcg ttgtttgatc gtctgggtga gtacattcca ttcattggcc tgaatactca
    31621 cggcggcgta attttcgcgt atctgggtgg gattgcgctg catgtctgga ccatcaaagg
    31681 ctatttcgaa accatcgaca gttcgctgga agaagcggct gcgctggatg gtgcgacacc
    31741 gtggcaggcc ttccgccttg tcctgttgcc gctgtcagta ccgattctgg cggtggtatt
    31801 catcctgtcg tttatcgctg ccattactga agttccggtc gcgtcgctgt tactgcgtga
    31861 cgtaaacagc tacaccctgg ccgtggggat gcagcaatac ctcaacccgc aaaactacct
    31921 gtggggtgac tttgccgccg ctgccgtgat gtctgcatta ccgatcacca tcgtcttctt
    31981 gctggctcaa cgctggctgg tcaacggcct gacggcaggt ggtgtgaaag gttaaagatg
    32041 ttgttctgcc aatgttatgc cgctgcaccc tcaacttacg ttatcccaac ttgtgactat
    32101 tattcggcgc tccacggagc gcctttttt
//
LOCUS       DAEEUE010000041        31766 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.041, whole genome shotgun sequence.
ACCESSION   DAEEUE010000041 DAEEUE010000000
VERSION     DAEEUE010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31766
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.041"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="KLK84_002017"
     CDS             complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="KLK84_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HBE2009344.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQ"
     gene            1794..2540
                     /gene="mipA"
                     /locus_tag="KLK84_002018"
     CDS             1794..2540
                     /gene="mipA"
                     /locus_tag="KLK84_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HBE2009345.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2630..3391
                     /locus_tag="KLK84_002019"
     CDS             2630..3391
                     /locus_tag="KLK84_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBE2009346.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3442..4326)
                     /locus_tag="KLK84_002020"
     CDS             complement(3442..4326)
                     /locus_tag="KLK84_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HBE2009347.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="KLK84_002021"
     CDS             complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="KLK84_002021"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBE2009348.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5747..6160
                     /gene="msrB"
                     /locus_tag="KLK84_002022"
     CDS             5747..6160
                     /gene="msrB"
                     /locus_tag="KLK84_002022"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBE2009349.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6202..6474
                     /locus_tag="KLK84_002023"
     CDS             6202..6474
                     /locus_tag="KLK84_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HBE2009350.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6844..7920
                     /locus_tag="KLK84_002024"
     CDS             6844..7920
                     /locus_tag="KLK84_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBE2009351.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDMVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7947..9326
                     /locus_tag="KLK84_002025"
     CDS             7947..9326
                     /locus_tag="KLK84_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE2009352.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9343..10388
                     /locus_tag="KLK84_002026"
                     /pseudo
     CDS             9343..10388
                     /locus_tag="KLK84_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10409..11245
                     /locus_tag="KLK84_002027"
     CDS             10409..11245
                     /locus_tag="KLK84_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBE2009353.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11250..12197
                     /locus_tag="KLK84_002028"
     CDS             11250..12197
                     /locus_tag="KLK84_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HBE2009354.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNEALDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12207..13187
                     /gene="ydjG"
                     /locus_tag="KLK84_002029"
     CDS             12207..13187
                     /gene="ydjG"
                     /locus_tag="KLK84_002029"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HBE2009355.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13324..14082
                     /locus_tag="KLK84_002030"
     CDS             13324..14082
                     /locus_tag="KLK84_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBE2009356.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14200..15558
                     /locus_tag="KLK84_002031"
     CDS             14200..15558
                     /locus_tag="KLK84_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE2009357.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="KLK84_002032"
     CDS             complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="KLK84_002032"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HBE2009358.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="KLK84_002033"
     CDS             complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="KLK84_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBE2009359.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="KLK84_002034"
     CDS             complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="KLK84_002034"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBE2009360.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19503..20054
                     /locus_tag="KLK84_002035"
     CDS             19503..20054
                     /locus_tag="KLK84_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HBE2009361.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20171..21214
                     /gene="selD"
                     /locus_tag="KLK84_002036"
     CDS             20171..21214
                     /gene="selD"
                     /locus_tag="KLK84_002036"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBE2009362.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDLNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVHARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21219..23180
                     /gene="topB"
                     /locus_tag="KLK84_002037"
     CDS             21219..23180
                     /gene="topB"
                     /locus_tag="KLK84_002037"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBE2009363.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23307..24347
                     /locus_tag="KLK84_002038"
     CDS             23307..24347
                     /locus_tag="KLK84_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBE2009364.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24464..25807)
                     /gene="gdhA"
                     /locus_tag="KLK84_002039"
     CDS             complement(24464..25807)
                     /gene="gdhA"
                     /locus_tag="KLK84_002039"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBE2009365.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            26043..26315
                     /locus_tag="KLK84_002040"
     CDS             26043..26315
                     /locus_tag="KLK84_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HBE2009366.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(26281..26688)
                     /gene="nudG"
                     /locus_tag="KLK84_002041"
     CDS             complement(26281..26688)
                     /gene="nudG"
                     /locus_tag="KLK84_002041"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HBE2009367.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQVLVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            26775..27395
                     /locus_tag="KLK84_002042"
     CDS             26775..27395
                     /locus_tag="KLK84_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBE2009368.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(27404..28711)
                     /gene="ynjE"
                     /locus_tag="KLK84_002043"
                     /pseudo
     CDS             complement(27404..28711)
                     /gene="ynjE"
                     /locus_tag="KLK84_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(28778..29431)
                     /locus_tag="KLK84_002044"
     CDS             complement(28778..29431)
                     /locus_tag="KLK84_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBE2009369.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(29431..30948)
                     /locus_tag="KLK84_002045"
                     /pseudo
     CDS             complement(29431..30948)
                     /locus_tag="KLK84_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(30921..31766)
                     /locus_tag="KLK84_002046"
                     /pseudo
     CDS             complement(30921..31766)
                     /locus_tag="KLK84_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         7957 a         7605 c         8240 g         7964 t
ORIGIN      
        1 tgctgttcca ggacgtagaa cagatggacc gggtttgccg ccacttctac atgatcgaag
       61 ttgaacacgc gggagaggat cttaaacgca aaacgcgtcg acagaccgtt catcccttca
      121 tcgacaccgg cgtagtcacg atattcctga tacgacttgg ctttcggatc ggtgtctttc
      181 agactttcgc catcataaac ccgcatcttt gaataaatgc tggagttttc tggctctttc
      241 aggcgcgaaa gaatggaaaa acgtgacagt gtttcgagcg tgccaggggc gcatggggcg
      301 tgagtcaatt cactgtgatt aagcaatttc tcgtagattt tgatctcttc ggaaatgcgc
      361 aagcaatacg gcaccttcac gatgtaaaca cggtcgagga aggcttcgtt gtttttgtta
      421 ttacggaaag tgacccattc ggacttgttc gagtgggcga gaataatccc gttgaacggc
      481 agggcggaga taccttccgt cccgttgtag ttaccttcct gagtggcggt taacaaggga
      541 tgcagcactt ttatcggtgc tttaaacatc tcaacgaatt ccatgatccc ctgattggcg
      601 cggcacagcg caccggaata gccgtaggcg tccgggtcat tctgcgcgta gtgttcgagt
      661 ttacgaatat cgactttccc aaccagcgcg gagatgtcct ggttgttctc atcaccgggt
      721 tccgttttgg cgatagcaat ttgttgcaga attgacggcc agaccttcac tacccggaac
      781 ttagtgatat cgccaccaaa ttcatgcagg cgttttgccg cccatggcga catgatggtg
      841 ccgagataac ggcgaggaat gccatactct ttttccagaa tctgagcatc ttcctgcgga
      901 ttgaaaagac agaacggatg atcgttgacc gggctacgct caccgttggc gctcaataca
      961 taaatcggta cgagctgcat taatgatttc agtcgctcag caagcgatga tttaccccca
     1021 cccacaggcc ccagcagata caggatttgt ttcttctctt ccagcccctg agccgcgtgt
     1081 ttcagataag agacaatctg ttcaatcgcg tcttccatgc cgtaaaactc ttcaaatgcc
     1141 ggataacgtg caatgacccg gttagaaaag agtcgagaaa gtctggggtc ctgggctgta
     1201 tcgaccatga caggctcacc gatagccatc aatagccgct cagccgcgtt ggcataagca
     1261 ctgcgatctt gccgacaagt ggtaagaaac tcctgcagtg tgaactcttc gtccttggca
     1321 gcttcatatc gctggcgata gtgatcaaat atattcatgg tatgccgtcc tttcgttttt
     1381 tagcacaggt taagagccgt tcatatgaat agtagaggct cccggaagag gtaaactgaa
     1441 cgacgtctct tgctaaaaca cagcaactct catgccattt tatcgacgat gagaacaacg
     1501 tgcccggtga tacaccttca aaattaagcg tagatggcaa atggaaaact tgcctgcacg
     1561 gctttcttaa tttcaatggc atatcaacaa ctcatccata aattttgcat aattaatgta
     1621 aagaccaggc tcgccagtaa cgctaaatcc atttggctgt aagcgcggtg tcatccgcgg
     1681 caggaaaatt aaacagttac tttaaaaaat gaaaacgtaa aaaggttggg tttcgatgta
     1741 ttgacgggta aactttgtcg cccgctaaac atttgtttaa ggaatgatta attgtgacca
     1801 aactcaaact tctggcactt ggagtgctta tcgcaacgtc tgcaggcgta gcgcacgctg
     1861 aaggtaaatt ttccctgggc gcaggcgtag gtgtcgttga gcacccatat aaagattacg
     1921 ataccgatgt ttacccagta ccggtaatca actatgaagg cgataacttc tggttccgtg
     1981 gcttaggtgg tggttactac ctgtggaatg acgcaacgga taaactttca attaccgctt
     2041 actggtcgcc gctttacttc aaagcgaaag acagtggcga tcaccaaatg cgtcacctgg
     2101 atgaccgtaa gagcaccatg atggctggtc tgtcttatgc tcactttacc cagtacggtt
     2161 acctgcgtac caccctggct ggcgataccc tggataacag caacggcatt gtctgggata
     2221 tggcctggtt gtatcgttac accaacggtg gcctgaccgt gactccgggt attggtgtgc
     2281 agtggaacag cgaaaaccag aacgaatact attatggcgt atcgcgcaaa gagtccgctc
     2341 gcagcggtct gcgtggctat aacccgaatg acagctggag cccttacctt gagctgagcg
     2401 ccagctacaa cttcctcggc gactggagtg tttacggtac cgcgcgctac acccgtctgt
     2461 ctgatgaagt tactgacagc ccgatggtgg ataaatcctg gactggcctg atttctaccg
     2521 ggatcaccta caaattctga taatgcatct ttccagggca ttttgtgcat aaaaacaggg
     2581 cgtcacgccc tgttttgcat tacggtggtg caatacctgg gaggaaacaa tgcaacaaaa
     2641 aatgattcaa tttagtggcg atgtctcact gccagccata gggcagggaa catggtatat
     2701 gggcgaagat gccagtcagc gcaaaacaga agttgctgca ctacgcgcag gcattgaact
     2761 cggtttaacc ctcattgata ccgccgaaat gtatgccgat ggcggtgccg aaaaggtggt
     2821 tggggaagca ttaaccggtc tgcgagagaa ggtctttctc gtctctaaag tctatccgtg
     2881 gaatgctggc gggcaaaaag cgataaatgc atgcgaagcc agtttacgcc gtctcaatac
     2941 tgactatctc gatctctact tattacactg gtctggcagt ttcgcttttg aagagactgt
     3001 tgccgcgatg gaaaaattga tcgcccaggg aaaaatccgt cgctggggcg tttctaacct
     3061 tggttatgcc gatatgcagg aactctggca gctgccgggg ggaaatcagt gtgccactaa
     3121 tcaggtgctt taccatctcg gttcacgagg gattgagtac gatctactcc cctggtgcca
     3181 gcaacagcag atgccggtga tggcttacag tccattagcc caggccgggc ggttgcgcaa
     3241 tggactgtta aaaaacgcgg tagtcaacga aattgcacat gtccaacaac atgcagctgc
     3301 gcttgaggtc gaactttcgt cagcggaatt agctatgctg gataaggcat atccggcacc
     3361 aaaaggaaaa actgcgctgg atatggtgtg atgttgatac gtgccggatg catcgcaccc
     3421 ggcacacaac attaacgcaa attaacgttt ggcaacgcga atggattgcg ccagatgtgc
     3481 aggtttctct ttggtgactt tttgcgtttc tgaagcgtaa gccgtttcta cacaaacaaa
     3541 ggtcttgtag ccatcgtccg gcatgtcgcc catgctaact gaaagcgccg gtcccgggtt
     3601 ccagccgaca acgttcagat gatgctggtg gcctacggcg ataatacgat tcagcgcttc
     3661 atcattaatc acgctgcaat cttgcggatt cagatacacg cgatcggtac ggtcagggaa
     3721 ggtctgaata ccgtcggtca gtacatcttc tttcgcgtca ttcactttat caatgaagcg
     3781 atcgcccagc ccactgacgc ttaccttagc gatatcaccc acgttaaagt aggtatgcag
     3841 ggcagaggtg gtttcaaatt cgccatgtga ttcaagatcg atttcacagg ttttacccac
     3901 gcggaaatgc gctaacagcg taaagtcgtg cggccagaat tttttcgtct cttcgctttg
     3961 tgtcaattca aaagtcagcg ctacgccatc agcatcttca tgatgtgatt tcagcgtcca
     4021 cggcaggttg cgggcaaaac cgtgcgcagg cagaccttgt tgtgccgccg gaccaaacca
     4081 cggccagcaa accggtacgc caccgcgaat agcgacgcca tttttgaacg gtgtgttgtt
     4141 gctcaaccac agaacttctt cttcacccgc aggtttccac gagagaaggt gtgcgccctg
     4201 taatgcaaaa gaggctttta cctggggatg atcgaccaca atgaggtcca gttcatccag
     4261 tttacgacgg gagaggacag gggagatttg ttcgatgacc ggaagggcaa aaattttctt
     4321 aatcatgacg cagtccttta acttcatttt atcaggtaaa aaaaagagcg accgaagtcg
     4381 ctctttttag atcacagtgt catctcaact tatttggaga tgtgagcgat caggtccaga
     4441 actttgttgg agtaaccggt ttcgttgtcg taccaggata ccagtttcac gaagttgtcg
     4501 ttcagagcga taccagcttt agcatcgaac acggaagtgc aaacttcgcc gttgaaatcg
     4561 gtagatacta cgtcatcttc ggtgtagccc agaacgcctt tcatttcgcc ttcagcagca
     4621 gctttaacgg cagctttgat ctgctcgtaa gttgcagctt tttccagacg aacggtcagg
     4681 tcaactacgg atacgttcgg ggtcggaacg cggaacgcca taccagtcag tttgccattc
     4741 agttctggca gtactttacc tacagcttta gcagcaccgg tagaggacgg gatgatgttc
     4801 tgggaagcgc cgcggccgcc gcgccagtct ttgtgagacg ggccatcaac ggttttctga
     4861 gtagcggtag tagcgtgaac ggtggtcatc agaccttcga tgatgccgaa gttatcgttg
     4921 ataactttag ccagcggagc taggcagttg gtggtgcagg aagcgttgga aacgatgtcc
     4981 tggccagcat atttgtcgaa gttagcgcct ttaacgaaca tcggagtgtt gtctttagac
     5041 ggaccagtca taaccacttt cttcgcacca gcggtgatgt gtttacgagc agtttcgtca
     5101 gtcaggaaca gaccagttgc ttcagcgaca acgtcaacac ccacttcgtc ccatttcagg
     5161 ttagccggat cacgttcagc ggtaacacgg atttttttac cgttaacgat cagatgaccg
     5221 tctttcactt caacggtacc gtcgaaacgg ccgtgagtgg agtcatattt cagcatgtat
     5281 gccatgtaat cagcgtctaa caggtcgttg attgcaacga tctcgatgtc agaacgtttc
     5341 tgagcagcac ggaaaacaat gcgaccgata cggccaaaac cgttgatacc tactttgata
     5401 gtcatatatt ccaccagcta tttgttagtg aataaaaggt tgcctgtaaa attacaaaaa
     5461 ccttacgcag cgtcaagcgg aatcgtgtca atcattgcga caaatcaatc ctgtgcctaa
     5521 gcattacgcg actgactcgc ctcacttttc ctttgggctt gagaccacat ggggtcggcg
     5581 ccccgaattt taaagggcaa ttacgataaa aatgtgattt tcatcacgat ttaggtgcag
     5641 ccatttcgtc gagatcaagt ttaggacaaa attttgccag ttggtgttaa tgttttgtta
     5701 gaatcagtca cgtaatgtga gcacgattaa agtgagatgt gagtaaatgg cgaataaacc
     5761 ttcggcagaa gaactgaaaa aaaatttgtc cgagatgcag ttttacgtga cgcagaatca
     5821 tgggacagaa ccgccattta cgggtcgttt actgcataac aagcgtgacg gcgtatatca
     5881 ctgtttgatc tgcgatgccc cgctgtttca ttccgaaacc aagtatgatt ccggctgtgg
     5941 ctggcccagt ttctacgaac cggtgagtga agaatccatt cgttatatca aagacttgtc
     6001 acatggaatg cagcgcatag aaattcgttg cggtaactgt gatgcccatc tggggcatgt
     6061 ctttcccgac gggccgcaac caacaggcga acgttattgt gttaactctg cttctttacg
     6121 cttcactgat ggcgaaaacg gcgaagaaat caacggttga aaaaacgatt cagcaactta
     6181 ttccacagga gcggaaaaat tatgaatctt gatgacatta tcaacagcat gacgcctgag
     6241 gtataccagc gtttgtcgac cgccgttgaa ctggggaaat ggcctgatgg cgttgcgttg
     6301 accgaggaac aaaaagagaa ttgcctgcaa ctggtgatgc tgtggcaagc ccgccataat
     6361 accgaagcac agcatatgac aattgacaca aatggtcaga tggtcatgaa gagcaaacag
     6421 cagttaaaag aagattttgg tatctcagca aagcctattg ccatgtttaa gtaagtggtt
     6481 catccccttt ttgccgcggc tatgaagggg atagccaaca atgaccccac gactcgtctc
     6541 aattatctcg acccgacctg gctaagtccc agtccgatct cttgcagtcg cgttcacgcc
     6601 tgaatgactt ctcctgaact taagtctcag acctatttgg ccggtaatcc ctctcgaatt
     6661 tgctcttttt gcgtcccgcc ttccaccaac ctgtaacaga cccagccatt ttctgcgtaa
     6721 ttgtgatctg agttgtaaaa tgcaggtcat tatctgtgtt gtgcagattt aatattgacc
     6781 tcaaaaatca tgtgggtaag tatgatggcc atctaagcaa aacaaaacac agaggatgac
     6841 gcaatgaaag cactggctcg gtttggcaag gcctttggcg gctacaagat gattcatgtc
     6901 ccacaaccca tttgtggccc ggaagacgta gtgattgaaa ttaaagccgc ggcaatctgc
     6961 ggcgcagaca tgaagcacta caatgtcgat agcggttctg atgagtttaa ctctatccgc
     7021 ggccatgagt tcgcaggttg tattgcgcag gttggtgaaa aagtcaaaga ctggaaagtg
     7081 gggcaacgcg tcgtgtcgga taacagcggt cacgtttgcg gcgtttgtcc ggcctgtgaa
     7141 cagggtgatt ttctgtgttg tacagaaaaa gtaaaccttg gtctggataa caatacctgg
     7201 ggcggtggtt tttccaaata ttgtctggtt cctggtgaaa ttctcaaaat tcatcgtcat
     7261 gcgttgtggg aaatccctga tggtgttgat tatgaggacg cagccgtact tgaccctatc
     7321 tgtaatgcct ataaatccat cgcgcagcaa tcgaaattcc ttcctggtca ggatgttgtc
     7381 gtcattggca ctggcccact cgggctgttt tccgtacaaa tggcgcggat tatgggggcg
     7441 gtaaatatcg tcgtcgttgg tctgcaagaa gatgtggcgg tccgcttccc ggttgcaaaa
     7501 gaactggggg cgacggcagt agtaaatggt tctaccgaag atatggtggc gcgctgccag
     7561 caaatttgtg gcaaagataa tctgggactg gtgattgaat gctccggtgc caatatcgca
     7621 ctgaaacaag ccatcgaaat gcttcgcccg aatggggaag tggtacgcgt tggaatgggc
     7681 ttcaaacctc ttgatttctc gattaatgac attaccgcct ggaataaaag catcattggg
     7741 catatggcct atgactccac ctcatggcgt aacgctatcc ggctattagc cagcggcgct
     7801 atcaaagtca aaccgatgat cacgcatcgt atcggcctgt cgcaatggcg cgaagggttt
     7861 gatgcgatgg tcgataaaac cgcaatcaaa gtgatcatga cttacgactt tgatgaataa
     7921 ctactctcct gaaggaacgt tatcgcatgg aacagataac aaaaccgcat tgtggtgcac
     7981 ggctggatcg cttaccggat tgccgctggc attcgtcaat gtttgccatc gtcgcgtttg
     8041 gcttgcttgt ctgctggagt aatgccgttg gtggcttgat cctcgcgcag ctgaaagcgt
     8101 tgggctggac agataattcc actacagcca cattctcagc aatcacgacc gccggaatgt
     8161 ttctcggtgc gctggtgggc ggcatcattg gcgacaaaac cggtcgcaga aatgcgttca
     8221 tcctctatga ggccattcat attgcctcga tggttgtcgg tgctttctca ccgaatatgg
     8281 attttcttat tgcctgccgt tttgtgatgg acgttgggct gggagcttta ctggttacac
     8341 tgtttgctgg tttcaccgaa tatatgcccg gtagaaatcg cggaacgtgg tccagtcggg
     8401 tttcctttat tggcaactgg tcatatccgc tctgctcact gattgcgatg ggactcacgc
     8461 cgctgattag tgcagagtgg aactggcggg tacaactgct tattcctgca atattgtcgc
     8521 ttatcgctac ggcgctggcc tggcgctact ttcctgaatc cccgcgctgg ctggaatcgc
     8581 gcggacggta tcaggaagcc gagaaagtca tgcggagtat agaagaaggc gtcatacgcc
     8641 agacgggtaa acctttgccg cctgtggtta ttgctgatga cggtaaagcg ccacaagcgg
     8701 tgccgtattc agccttactg acaggagtat tactgaaacg cgtgatatta ggctcttgtg
     8761 tgctgattac catgaacgtt gtgcagtaca cactaattaa ctggttgcca acaatattca
     8821 tgacccaggg gattaattta aaagactcga ttgttttaaa taccatgagt atgtttggcg
     8881 cgccatttgg tatttttatt gccatgctgg tgatggataa aattccgcgt aaaacaatgg
     8941 gtgtggggct attaatcctg attgcggtgc tcggatatat ctattcactg caaaccagta
     9001 tgttgctcat aacgctgatt ggtttcttcc tgattacttt cgtctatatg tacgtttgct
     9061 atgcctcggc agtgtatgtc cctgaaatct ggccgacaga ggccaaactc cgtggctccg
     9121 gtctggcgaa tgcggtaggg cgaatcagtg gtattgccgc accttatgcc gttgcagtgc
     9181 tgctcagtag ttatggtgta acgggagtct ttattcttct gggggctgtt tcaaatattg
     9241 tcgcaattgc tatcgccacc attggaattg aaaccaaagg tgtctccgtt gaaagtttaa
     9301 gtattgatgc agtagccaat aaataatacg ggtggattca aaatgaaaaa ttcaaaagca
     9361 atattgcagg tgccgggcac aatgaaaatt atttcagcag aaataccagt gcctaaagaa
     9421 gatgaagttt tgattaaagt agaatatgtc ggtatttgtg gttcagatgt acatggtttt
     9481 gaatcaggcc cgtttattcc gcctaaagac ccaaatcaag aaattggcct gggtcatgaa
     9541 tgcgccggga cggttgtggc tgtgggaagc cgcgtgcgca aatttaaacc gggggatcgg
     9601 gtaaatatcg aacctggcgt tccttgcggt cactgtcgtt actgtctgga aggcaaatat
     9661 aacatctgcc cggacgttga ttttatggcg acacaaccca actaccgcgg cgcattaacg
     9721 cactatctgt gtcatccgga gagctttact tacaaactgc ccgacaatat ggacacgatg
     9781 gaaggggcgc tggtggagcc tgccgcagtc gggatgcatg ccgcgatgct ggcagatgtt
     9841 aaaccgggta agaagataat tattctggga gcaggttgta ttggtttgat gacgttgcaa
     9901 gcgtgcaaat gcctgggcgc aacggaaatt gccgtcgttg atgtactgga aaaacgtctg
     9961 gcaatggggg aacagcctgg tgcgacagtg gttattaatg gcgcaaaaga ggacactatt
    10021 gcacgctgtc agcaatttac cgaagacatg ggcgcagata ttgttttcga aacagcgggt
    10081 tctgcggtca ccgttaaaca ggcaccttta atctggtaat gcgcggcggt aaaattatga
    10141 ttgttggtac tgtacccggc gattcggcaa tcaatttcct caaaatcaat cgcgaagtca
    10201 ctatccagac ggtattccgc tatgccaatc gttatccggt cacgattgaa gctatttctt
    10261 cagggcgatt cgatgtgaaa tcgatggtga cgcatattta cgattatcgg gatgtacaac
    10321 aggcatttga agagtcagtt aacaacaaac gcgacattat taaaggcgtt attaaaatta
    10381 gcgattaatt aatacgggag aacagaacat gctcgcagat atcagatatt gggaaaacga
    10441 tgccacaaat aaatattatg caattgccca ttttaacgta tggaatgcag aaatgttgat
    10501 gggcgttaaa gacgcagccg aagaagcgaa atccccggtt attatttctt ttagtacagg
    10561 ttttgtcggt aacacctcat ttgaagattt ctctcacatg atggtatcaa tggcacaaaa
    10621 agcaacggtg ccggtaataa ctcattggga tcatggtcgg agtattgaga ttattcataa
    10681 cgcctggact catggtatga attcattaat gcgtgatgct tctgcatttg atttcgaaga
    10741 aaatattcgt ttaaccaaag aggctgttga tttctttcat ccgctgggta ttccggtaga
    10801 ggcggaatta gggcatgtcg gtaatgaaac cgtttatgag gaagcgctgg cgggctatca
    10861 ctataccgat cctgaccagg cggctgaatt tgttgaacgt acgggctgtg actcattggc
    10921 tgtcgccatc ggcaaccagc atggggttta tacgtcagag ccgcaattga actttgaggt
    10981 cgtcaaacgc gtacgcgatg ctgtttctgt tccgctggtt ttgcatggcg catcggggat
    11041 cagtgatgcc gacattaaaa ctgcaatttc gttgggtatt gcgaaaatca acattcatac
    11101 ggagctctgt caggccgcaa tggtagcagt gaaagaaaac caggatcagc ctttcctgca
    11161 tttagaacgt gaagtacgta aagctgtaaa agaacgggca ttggaaaaaa ttgaactgtt
    11221 cggttctgat ggcaaagcgg aataatgata tggataatct cgacgttatt tgtataggtg
    11281 ccgctattgt tgatattcca ttacaaccgg tcagtaaaaa tatctttgat gtagattctt
    11341 atcctcttga aagaatcgca atgagcaccg ggggcgatgc aataaatgaa gcaacaatta
    11401 tttctcgtct ggggcatcgt accgcattaa tgagccgcat tggtaaagat gcctcagggc
    11461 aatttattct cgaccattgt cgcaaagaga atattgatat tcaaagcctg aaacaggacg
    11521 tcagcataga tacatctatt aacgttggac tggtgacgga ggatggcgag cggacgtttg
    11581 tcacgaaccg taacggcagt ttgtggaaat taaatattga cgatgttgat tttgcgcggt
    11641 ttcctcaggc gaaattatta tcactggcca gtattttcaa cagtcctcta ttggatggta
    11701 aagcgctaac agaaattttt acgcaagcga aagcccggca gatgatcatt tgtgccgata
    11761 tgatcaaacc gcggttgaat gaagcgctgg atgatatttg cgaagcattg agttatgtcg
    11821 attatctgtt tccaaatttt gccgaggaaa aattactcac agggaaagag acactggatg
    11881 aaattgctga ctgctttctt gcgtgcggcg taaaaacggt ggtgattaaa acgggtaaag
    11941 acggctgctt tatcaagcgt ggtgacatga cgatgaaggt gccggcggtc gcaggaataa
    12001 ccgccatcga caccattggc gcgggcgata actttgcttc aggttttatt gcggcactgt
    12061 tagaaggcaa aaatctgcgt gaatgcgcac gctttgccaa tgcaacggcg gctatctcgg
    12121 ttctaagcgt cggtgccacc accggcgtaa aaaacagaaa gctggtcgaa caattgctgg
    12181 aagaatacga aggataatga aggcaaatga aaaagatacc tttaggcacg acggatataa
    12241 cgctttcgcg aatggggttg gggacatggg ccattggcgg cggtcctgca tggaatggcg
    12301 atctcgatcg gcaaatatgt attgatacga ttcttgaagc ccatcgttgt ggcattaatc
    12361 tgattgatac tgcgctagga tataactttg gcaatagtga agttatcgtc ggtcaggcgt
    12421 taaaaaaact gccccgtgaa caggttgtag tagaaaccaa atgcggcatt gtctgggaac
    12481 gaaaaggaag tttattcaac aaagttggcg atcggcagtt gtataaaaac ctttccccgg
    12541 aatctatccg cgaagaggta gaagccagct tgcaacgtct gggtattgat tacatcgata
    12601 tctatatgac gcactggcag tcggtgccgc cattttttac gccgatagct gaaactgtcg
    12661 cagtgcttaa tgagttaaag gctgaaggga aaattcgcgc tataggcgct gctaacgtcg
    12721 atgctgacca tatccgcgag tatctgcaat atggtgaact ggatattatt caggcgaaat
    12781 acagtattct cgaccgggca atggaaaacg aactgctgcc gctatgtcgt gataatggca
    12841 ttgtggttca ggtttattcc ccgctagagc agggattgtt gaccggcacc atcactcgtg
    12901 attacgttcc gggcggcgct cgggcaaata aagtctggtt ccagcgtgaa aacatgctga
    12961 aagtgattga tatgcttgaa cagtggcagc cactttgtgc tcgttatcag tgcacaattc
    13021 ccactctggc actggcgtgg atattaaaac agagtgattt aatctccatt cttagtgggg
    13081 ctactgcacc ggaacaggta cgcgaaaatg tcgcggcact gaatatcaac ttatcggatg
    13141 cagacgcaac attgatgagg gaaatggcag aggccctgga gcgttaaata ctcaacacga
    13201 ttcacctctg tttctgtgaa aactgtgatg aaaatctgtg attcaaatag gttattttga
    13261 aagtaaacat cggttatgcg ataatcgcgc taatgtgatg taaaaagata caggggtgta
    13321 atcgtggcgg caaaagacag gattcaggca attaagcaaa tggttgccaa cgataaaaag
    13381 gtgacagtct caaatttgag tgggattttt caggtaaccg aagaaaccat tcgccgcgat
    13441 cttgagaagc tggaagatga aggctttttg accagaacct atggtggtgc tgttttaaat
    13501 acagcggtgt tgacggagaa tatccatttt tataagcgcg cttcatcgtt ttatgaagag
    13561 aagctgctta ttgcacgcaa ggcactaccc tttatcgaca ataaaaccac catggcagcc
    13621 gattccagta gtaccgtaat ggaattgctc aaattgttac aggaccgtag tgacctgacg
    13681 ttgctaacca actcggcgga agcgattcat gttctggctc agtcagaaat taaagtcgtt
    13741 tcaacgggtg gggaactaaa caaaaataca ctttccctgc aaggaagaat tactaaagag
    13801 atcatcagcc gctaccatgt cgatatcatg gtaatgagtt gtaaaggtct tgatattaac
    13861 agcggcgcgc tggactctaa tgaagcggaa gctgaaatca aaaagacaat gatccgtcag
    13921 gcgacagaag ttgcgttatt ggttgatcac tctaagtttg atcgtaaagc ttttgtccag
    13981 ttagctgact ttagtcatat taattatata ataactgata aatcaccggg tgcagagtgg
    14041 atagcatttt gcaaagacaa taatatccag ctggtgtggt aatttaagca cacgttgtca
    14101 atattatttg ttcctgcgac tggaaataaa atgatgataa agatgcggca agtgaatttg
    14161 ccgcaattgc taccttttat agcaaatccg aggcacccaa tggaacaata tgatcaaatt
    14221 ggcgcaagac tggaccgctt gcctttggcc cggtttcatt atcgtatatt tggtataata
    14281 agctttagtc tgttattaac ggggtttttg agttactcag gtaatgtcgt cttagcaaag
    14341 ctggtaagca atggatggtc aaataatttc ctctatgccg cctttacctc ggcattaatg
    14401 tttggttatt tcatcggctc acttactggt gggtttattg gtgactactt tgggcggcgc
    14461 agggcgtttc gcataaatct tctcatcgtc ggtattgctg caacaggggc cgcttttgtc
    14521 cctgatatgt actggctcat tttctttcgc ttcctgatgg gaacaggaat gggggcgctg
    14581 attatggttg gctatgcctc atttacggag tttatccccg cgacggtgcg tggaaaatgg
    14641 tccgcgcggc tctcatttgt tggtaactgg tcgcccatgc tgtctgcggc gataggcgtg
    14701 gtggttatcg ctttttttag ttggcgaata atgtttcttc tgggtggtat tggcatactg
    14761 ttagcctggt ttctctcagg taaatacttt atcgagtcgc cacgatggct ggaagggaaa
    14821 gggcaaatcg ccggtgcaga aagccaactt cgtgaagtag agcagcaaat tgaaagggag
    14881 aagagtattc gtttacccca gcttactttg aaccagagca acagcaaggt taaggtaatc
    14941 aagggtacct tcaggctcct gtttaaaggg gaaatgttac gacgtacatt agttgcgata
    15001 actgttttaa ttgcaatgaa catttcgctt tataccatta ccgtatggat accgaccata
    15061 tttgttaact ccggcattga tgtcgataaa tcaatattaa tgaccgctgt tattatgatt
    15121 ggtgctccgg taggaatatt tattgcagca ttaattattg atcattttcc tcgtcgatta
    15181 tttggctccg ccttacttat tattattgcc gtgttaggct atatctattc aagtcagact
    15241 acagagtggg cgattttaat ctatggtctg gtgatgatct tctttttata catgtatgtt
    15301 tgcttcgcgt cggcggttta tatcccggag ctttggccaa cgcatttacg cctgcgcggt
    15361 tcgggtttcg ttaatgccgt cggacggatc gtcgcagttt tcacgcccta tggcgttgcg
    15421 gcattattaa cacattatgg gtcgatcacg gtgtttatgg tgcttggtgt catgttagtg
    15481 ctctgtgcgc tggttctctc catttttggc atcgaaacgc ggaaggtgtc gctggaagag
    15541 atttctgagg tgaattaaat ctttcgttga tggcatcaat tgccagatgt accactgacg
    15601 tgttttattt ggcctacaat gccagtacag aattgtaggc cagcgtaaaa ttacccctgc
    15661 gtctcttccc agtctgccag cgtatatagc gttgccccag ctgctgacat ctccataaac
    15721 gcgtgtgcac tgtcctgggg ctggatattc acgccacgac agccatcggt aatcacgttt
    15781 accttataac ctaactgtaa cgcgtccagc acggtaaact tcacgcaata gtcagtagcc
    15841 aggcccataa cgatcaattc atcgatttca tgatcgcgca accagtcatc aagcgcggtt
    15901 ttctgccgac gtccgttatc aaaaaaggca ctgtaactgt caactaaagg attttcgcct
    15961 ttatggaaca ccgctgcgat ctctttttga ttcagtaacg gatgtaattg tgcgccttca
    16021 ctgttctgca cacagtgttc aggccagaaa gtttgccgca ggccgccgag ttggcctggc
    16081 gtataaggct ctacgccgtg ctggctggca aagctgccgt gattcgccgg atgccagtcc
    16141 tggctggcga taaccgcttc tccgcgcgac tggcaccagt caataaggcg gttagcaacc
    16201 tccaccgtac tgtcaccttc cggtacggcg agcgcgccac cagcacagaa atcattttgt
    16261 aaatcgacca gtaacagggc gcgatggggc attgcagtct ccttaatcat ccggcgtcag
    16321 ttcgccgcgc aggttttggc tcatggcctt gcgaatggtt tcagtatcca gttcctggct
    16381 cagcaggtaa tgcagtttgg ttagcgtggc ttctacagtc atatctgcac cgtcaataac
    16441 gccggcatgg gcgagggcgt taccggtggc ataaccactc atattcactt taccggacat
    16501 acattgtgtc aggttgacca ccacaatacc gcgatcgctg gcttcttgta attcctgcag
    16561 gaaggctttg ttttgtggcg cattacccac gccataggag cgcagaatca atgctttcac
    16621 cggttggcgc agaaaattgc gcacgacgtc agcagaaatc cctggataaa tcgtcaccac
    16681 gccaattggt tgtggcgtga ttggatgaac gattaattcc ccttcaccgt gcggggcggg
    16741 tggcgtattc aaacgacgaa tatggatacc agcttccagt aacggaggaa ggtttggaga
    16801 ggcaaacgca tcaaaaccat cggcatgggc tttggtagtg cggttgccgc gatacaatcg
    16861 gttattgaaa aagagcgtta cttcgttgat cggataattc gccgcaacgt acaacgcatt
    16921 cagcagatta atttgtccgt cagagcgtaa ctcagccagc gggatttgtg accctgtcac
    16981 aatgaccggt ttaccgagat tctcgagcat gaacgacagc gcagaggcgg tatacgccat
    17041 cgtgtcggta ccgtgtagaa tgacaaaacc atcatagtcg tcatagtgcg ctttaatatc
    17101 ttcagcaata tgctgccagt cttccggcgt catatcagaa gaatccatca acggcgtata
    17161 ttcatgaatg gtgaaatctg gcatctccgg gcgatggaat tccggcatca gcgccagttg
    17221 gcgttgtaga tgacctgaca ccggaatata accctgctcg gaacgctgca tcccgatggt
    17281 cccgcccgtg taggcgacgt aaattgattt cttttgcatg atattgatat gaatgatatc
    17341 gaaagagcaa aagtatatac gtgaggcagg aggagcgcac ctgatactat gcggataagg
    17401 aaatatgggg cgagacagcg ggaagaggat tcgtcgaggt aaaaaataat gtggccgacg
    17461 atctgtcggc cactcagtac aagacttaac gcacgttggc gcaggtcagg caaaacgcat
    17521 aacggttttg tgggtcgtta aacgcggcca gcttgtcgct ttcactttta acggtagagg
    17581 ctaccgaggc aagcggtgca ggtaacatgg cctggaacgc atctggcaac attgcccgga
    17641 cagaaccaga catgttgtcc atcactttgt cgaagaaggt cggttcatca acgtagtatt
    17701 ccagatgcca ctgtttcact tttgcgagct ctgctgcttt ggcaaccgca tcatcgaaat
    17761 ccccgagact atcgaccagc ccgttagctt ttgcatcctg accggtccag acgtggccct
    17821 gggcgatttt atcaatttgc tccggcgtcg aatgacgcgc atcagcaacc agcgtgataa
    17881 agcgtttata gccattctca atgcttaatt gcatcatctg ctgcgcttcc ggcggcagtg
    17941 ccctggtgat agaaacatcc gccagcggtg aagttgagac accatcagta tgaacaccaa
    18001 tcgaatccag actattttct acggtggtga tcacgccgaa gataccgata gaaccggtca
    18061 gggtgctggg gttagccaca atgtaattag ctggcgtgga aatccagtaa ccaccagatg
    18121 ccgccatgcc gcccatcgat acaaccacag acttacccgc tgcccgggct gctgccagtt
    18181 cagcgcgaat cacttcagac gcggtaacgc tgccgcctgg gctattaaca cgcaggacaa
    18241 tcgctttcac tttcgggtca aggcgagcgt cgcggatttg tgccgcagtg gtatcaccgc
    18301 caacattccc ctgagtttcc tcgccatcca taattgcgcc attagcgaag acgacaccga
    18361 tgctgtcacc ggtatctgcc ggcgttttca atgcgtaatc gtaataactg atggcgcgat
    18421 aatttttatc agtcttactc cagccgaact ctttggtcag agctttttcg atttcggcac
    18481 tcgatgccag tgcatcgacc agcttgtttt ccagtgcata tttcgcggta tcgccaccgg
    18541 ttttggttaa accctcaagc aacccttgcg cgccagggaa tacctgctga gcagggatct
    18601 gccggttagc ggcaacagta ttcagatagt tttgccacag ctcaccaatc cagcggctgt
    18661 cagcttcgcg ggctgccggt gacatatcat cacgaataaa cggttcaacg gcagatttat
    18721 acgtacccac gcggaacaca tgggtggaaa ctttcagctt atccagcaac gatttgtagt
    18781 acagaccgtt ggtagcaaaa ccgtgcagat caaccacgcc ttgcggtgac agccaaattt
    18841 tattggcgaa actggcgaga taatattgcc cctggctgta gttctcgcca acggcataaa
    18901 ccggtttccc gctgtcacga aactctttca gggctttgcc gatgtactgc atagacggct
    18961 ggtcgccgcc tgcgaagttt ttcagatcca tcacgatacc ggtgatattg cggtcgtcct
    19021 tcgcctggcg aatggtgttg acgatatcaa acagtgagtt ttcctgcaga cgatcggaac
    19081 tggcaccaag cagctggcgg cttaatttac taaaccgctg agaactgtct ggtttatcga
    19141 cgatcacacc agaaatgtcc agcagcagtg cgccacgact ggccgtttct ttcgaatcac
    19201 caccactgac ctgcatccag atccccacac caaccagtac gaggaaaata aagaacaggt
    19261 taagtaccat ttcacggacg aaattcagca gacgccacgt ccatttaaaa aatccggcaa
    19321 taaatcgcca aagggttcgc atgtattctc ccaacttaag gtcacacctg tcacaggcgc
    19381 aatagatgtg gttatcgtaa tgacccaacc ggcacttgtc agcaggaatt gcggactgtg
    19441 ctgtaacaaa atccactctc gtgttaattt tgtgaataaa tatcacgaca ggagttaatc
    19501 aaatggatgc actcgaacta ttgatcaatc gccgtagcgc ctcccgcctg gctgaacccg
    19561 cgccaacggg tgaacaactg caaaacatcc tgcgtgcggg tatgcgtgcg ccggaccata
    19621 agtccatgca accgtggcat ttttttgtga ttgaagggga agggtgcgag cgtttcagcg
    19681 ccgtactgga acagggggcg attgctgccg gtagtgatga taaagctatc gacaaagccc
    19741 gtaatgcgcc gttccgcgca ccgctcatca tcacggtggt ggcgaaatgc aaagaaaatc
    19801 ataaagtccc gcgatgggaa caggaaatgt ctgccggatg cgcggtcatg gcgatgcaaa
    19861 tggcagccgt tgcccaggga tttggcggca tctggcgcag tggcgcatta actgaaagtc
    19921 cggtagtgcg tgaagcattc ggttgccgtg agcaggataa aattgtcggt tttctctacc
    19981 tcggcacgcc gcagttgaaa gcatctacgt cgattaacgt cccggacccg acgccgtttg
    20041 taacttattt ctgataattg tcgcgaaact gtctggattg tgagcaaagg cgcaggaatt
    20101 cagacactct cacttatcac ttcacggaat gagggttacc atacccggat tgcattgaca
    20161 ggagatgtcc atgagcgaga actcgattcg tttgacccaa tacagccacg gagctggttg
    20221 cggctgtaaa atttccccaa aagtgttgga aaccatcctg cacagtgagc aggcgaagtt
    20281 tgttgatctg aatttgcttg tgggtaatga aacccgcgac gatgcggcgg tgtacgatct
    20341 gggcaatggc accagcgtta tcagtaccac cgacttcttt atgccgatcg ttgataatcc
    20401 tttcgatttt ggccgcattg cggcgactaa cgccatcagc gatatcttcg cgatgggggg
    20461 caaaccgatt atggcgattg cgatcctcgg ctggccgatt aacaaacttt ccccggaaat
    20521 tgcccgcgaa gtaaccgaag gtggacgcta tgcatgccgt caggcgggta ttgcgctggc
    20581 tggcggtcac tccatcgatg cgccggagcc gatttttggt ctggcggtaa cggggatcgt
    20641 accgaccgag cgggtgaaga aaaacagtac cgcacaagcc ggatgcaaac tgttcctgac
    20701 gaaaccgctg gggatcggcg ttcttaccac ggctgagaaa aaatcactgt tgaaaccaga
    20761 acatcaggga ctggcgacgg aagtgatgtg ccggatgaac atcgcaggcg cgtcctttgc
    20821 caacatcgaa ggcgtaaaag cgatgaccga cgttacgggc tttggtctgc tgggccactt
    20881 gagcgaaatg tgtcaggggg ctggtgtgca tgcacgcgtc gactatgaag cgatcccgaa
    20941 actccccggt gttgaagagt acattaagtt gggcgcagta cctggcggca ctgaacgtaa
    21001 ctttgccagc tacggtcatc tgatgggtga aatgccgcgt gaagtgcgcg atctgctgtg
    21061 cgatccgcaa acttctggcg gtttgctgct ggcggtcatg ccggaagcag aaaatgaggt
    21121 taaaactaca gccgccgagt ttggcattga actgacggcg attggcgaac tggtgccagc
    21181 gcgcggcggt cgtgccatgg ttgagatccg ttaatttaat gcggttgttt attgccgaaa
    21241 aaccgagtct ggcgcgcgcc attgctgatg tcctgcccaa accgcaccgg aaaggcgatg
    21301 gctttatcga gtgcggtaat ggtcaggtgg tgacttggtg tatcggtcac ctgcttgagc
    21361 aggcgcagcc agacgcctac gacagccgct atgcgcgctg gaatcttgcg gatttgccga
    21421 ttgtcccgga aaagtggcaa ttacagccac gaccctccgt gaccaaacaa cttaacgtca
    21481 tcaaacgctt cctgcatgaa gccagcgaaa tcgttcacgc cggggacccg gatcgtgaag
    21541 ggcaattgct ggtggatgaa gtgctggact atctgcaact ggcaccggaa aagcgccagc
    21601 aggtgcagcg ttgcttgata aacgacctga acccgcaggc ggttgagcgg gcgatcgacc
    21661 gtcttcgttc caacagtgag tttgtgccat tgtgcgtttc tgcgctggcg cgagcgcgtg
    21721 ccgactggct gtacggcatc aatatgaccc gtgcgtatac cattctcggt cgcaatgccg
    21781 gttatcaggg cgtactttcc gtgggacgcg tgcagacacc cgtgctcggg ctggtggtgc
    21841 gccgcgatga agagattgaa aacttcgtgg cgaaagactt ctttgaagtc aaagcacata
    21901 tcgtgacacc tgccgatgag cggtttaccg ctatctggca accgagcgaa gcgtgtgaac
    21961 cgtaccagga tgaagaaggg cgcttgttac atcgtccact ggcggagcat gtggttaacc
    22021 gcattagtgg tcaaccggct attgtcacca gctataacga taaacgggaa tcagaatccg
    22081 cgccgctgcc gttttcgctt tcggcgttgc agattgaagc ggcaaaacgc ttcggtctga
    22141 gcgcgcagaa cgtgcttgat atctgccaga agctgtacga aacgcacaag ctaatcactt
    22201 atccgcgttc cgattgtcgc tatttgccag aagaacattt tgccggacgc cacgcggtga
    22261 tgaatgccat cagcgttcat gcaccggatc tgttgccgca gccagtggta gatccagata
    22321 tacgcaaccg ctgttgggat gacaaaaagg tcgatgcgca ccacgccatc atcccgaccg
    22381 cacggagttc tgcgatcaac ctgacggaga acgaagcgaa ggtctataac ctgattgccc
    22441 gtcaatacct gatgcagttc tgcccggatg cggtgttccg caagtgtgtt atcgaactgg
    22501 acattgccaa aggcaaattt gtcgctaaag cccgttttct tgctgaagca ggctggcgca
    22561 cgctgttagg cagcaaagag cgcgatgaag aaaacgacgg cacgccattg cctgtggtgg
    22621 cgaaaggcga tgagttgctg tgtgaaaaag gtgaagtggt agagcggcaa acccagccgc
    22681 cgcgccattt taccgatgca acactgcttt cggcgatgac cgggatcgcg cgctttgtgc
    22741 aggacaaaga tctgaaaaag gtccttcgtg cgaccgatgg tctggggaca gaagcaaccc
    22801 gtgccgggat tattgaactg ctgttcaagc gtggttttct gaccaaaaaa gggcgctata
    22861 tccactccac cgacgccgga aaagcgttat tccattcgct gccagaaatg gcgacgcgac
    22921 cggacatgac cgcgcactgg gaatcggtgc tgacgcaaat cagcgaaaag cagtgtcgct
    22981 atcaggactt tatgcagccg ctggtgggga cgctatatca gcttattgat caagccaaac
    23041 gtacgccggt gcggcagttt cgcggcattg tggctccggg cagtggtggc agtgctgata
    23101 agaaaaaggc tgcaccgcgt aaacgtagtg cgaaaaaaag tccgccagca gatgaagccg
    23161 gaagcggggc gatagcgtaa gcgagcgaat ctttcgtgct attcgagtca tattctgaaa
    23221 tatccagcgg atcaagaaaa ttcgttggat atttttttgc atggataaaa ttatcgcctc
    23281 taaagtatgt aataacaggg aatgtgatga aaaaagtttt attgcaaaac catcctggga
    23341 gcgagaagta ttcttttaat ggctggggaa tatttaatag taattttgaa cgggtgatta
    23401 aagaaaataa ggccatgctg ctttgtaagt gggggtttta tttaacatgt gttgtcgctg
    23461 taatgtttgt attcgcagcg ataacatcca acggtttgaa tgaaagaggc ctgattaccg
    23521 cgggatgctc ttttctttat ctattaatta tgatggggct tattgttcgg gccggtttta
    23581 aagcaaaaaa agaacaactg cattattatc aggctaaagg tattgagccg ctcagtatcg
    23641 agaagttaca ggcgctacaa ttgatcgcac cttatcgatt ctatcataag caatggtctg
    23701 aaacgctaga gttctggccg cgaaagcctg aacctggcaa agataccttc caatatcatg
    23761 tgcttccttt tgactcgatc gatatcatca gtaaaagacg cgagtcttta gagaatcaat
    23821 ggggtatcga agatagcgaa agttattgtg ccttaatgga gcattttctt tctggcgacc
    23881 atggagccaa tacctttaaa gcaaacatgg aggaagcccc agagcaggtt atcgccttgt
    23941 tgaataaatt tgctgttttt ccctcagact atatctctga ttgcgctaat aatcgctccg
    24001 gtaaatcctc ggcgaagcta atatgggcgg cggaattatc atggatgatc tcgatatcaa
    24061 gcacagcttt tcaaaacggg acaattgaag aagaactggc ctggcattat ataatgcttg
    24121 cttctcgaaa ggcgcacgag ttgttcgaaa gcgaagaaga ttatcaaaaa aatagtctaa
    24181 tgggatttct ttactggcat atctgctgct atcgcagaaa gttaacggat gctgaacttg
    24241 aagcgtgtta tcgttacgac aagcagtttt gggagcacta cagtaagaaa tgccgttggc
    24301 ccataagaaa tgttccctgg ggagcatcat ccgttaaata ctcataaacg cctgaaattt
    24361 tgccgggggc gctgcgcttg accggcctac attagagcgt aactgcaatg aattgtgccc
    24421 atctgtaggc ctgataagcg tagcgccatc aggcatttac aacttaaatc acaccctgcg
    24481 ccagcatcgc atcggcaacc ttcacaaaac cggcaatgtt cgcgccctgc acgtagttgg
    24541 tttgctcacc ttcaccacca tgctcaacac aggcatggtg gatatccagc atgatgtgat
    24601 gcaaacgtgc gtcaactttc tcggctttcc agcccaggcg cgcagcgttt tgtgccattt
    24661 ccaggcccga cgttgctaca ccgccagcat tagccgcttt acccggtgca aacagtacgc
    24721 ctgcctgctg gaacagttca gtcgcttcga tggtggtcgg catatttgcc ccttcagcga
    24781 cggctttaac gccattggcg ataagctgat gcgcggcgtc aacatccagt tcgttctggg
    24841 tggcgcaagg cagggcgata tcaaccggta cagaccacgg ctgttggcct tcgagataga
    24901 ccagaccaaa ttctttggcg taatctgcca ctcgaccatc gcggctggct ttgatttcga
    24961 taagacgtgc cagtttctct ttcgtgaatc cgctttcatc aactacagtg ccgctggagt
    25021 ctgacgcagt gatcacacga gcaccaaatt ccatcgcttt ttcgatagcg tactgggcga
    25081 cgttgccgga gccagaaacg gaaacgctca tcccttcaaa acccataccg tggcgtttta
    25141 acattgcttc tgtgaaataa accagaccgt agccggtagc ttccgggcga ataagactac
    25201 cgccaaatga aaggccctta ccggtgaaga cgcaggcggt attgttggag agctttttca
    25261 tcatccccgc cataaagccg acttcacgac caccaacgcc gatatcacct gccggaacgt
    25321 cggtatccgc gcccaggtgg cgatacagtt cagtcatcag cgtctggcaa aaacgcatca
    25381 ctttaccttc gctttttcct ttcggatcga aatcgctgcc gcctttacca ccgcccatcg
    25441 gcagggtagt cagggcattt ttgaaggttt gttcaaagcc gaggaatttg agaatggaaa
    25501 ggttaactga cggatggaag cgcataccgc ctttgtacgg gccgatggca gagctgaact
    25561 gcacacgcca tgcacggttg acctgtatct ggttgcgatc atcaacccat accacgcgaa
    25621 actggatcac gcgctccggt tcaaccagac gctccagtaa tgacatctgg cgatattttg
    25681 gattttgttc aagaaaaggc cagagtgtgg tcattacttc acgaacggct tgcgcgaact
    25741 cggtttgatt cgggtcgcgc ttttggacat ggttgaggaa tgactccaga gaatatgtct
    25801 gatccataga tataaaaacc ttatatatta atacgattgt gcttatgttg tttatgtcat
    25861 gtgcttttgc agttttcgac tataccatca agaatgtgac ggtaagcaag aaaaaaatga
    25921 cccaggaaag caaattaata acgagagtaa tctcataacc aaatgcgata actcagacgc
    25981 taaagttttg ccacccggct accgttatca gtagcagaga caataacgtt aaggagttcc
    26041 ctgtgagtcg agcattgttc gccgtagtat tagcatttcc attaattgca ctggctaacc
    26101 cgcattatcg tccggacgtg gaggtaaacg taccgccaga agtgttcagt tcaggcgggc
    26161 agtcagcgca gccctgtact cagtgctgcg tttatcagga tcaaaattat tctgaagggg
    26221 cggtaattaa agccgaagga atactgctgc aatgccagcg tgatgacaaa acccttagca
    26281 ctaatccgct gctctggcgg cgcgtaaagc cataaacgcc tctaataatg gaatgtcagc
    26341 aggggccagc ggatattgca gcgcctcttc aggtgagcac cagaccagcg cctgatgttc
    26401 atgtgcctgt aacgtcccgt ggaagtcggg tacgtgccag gcatgaagat ggataatccg
    26461 gcccgaaact tctcgctgat ggctggcaac atactcaccc acagttgctt cgatgtccaa
    26521 ttcttcgttt aactcacgca ccagcacctg ctgctggctt tcatccagct cgactttacc
    26581 tccggcaaac tcccataatc ccgcctgatc gctctgggcg gggcgttgcg cgagtaaaat
    26641 tttgccatca cgttcaatga tggcggcaac aacttcaatc attttcataa attacacacg
    26701 ggcagataag cgaaaaatgc cataatatcc gcactgttgc tcaatcagaa aggctttata
    26761 tttaaggagt gcctgtgcta gaccgccatc ttcatccccg gattaaaccg ttgttgcatc
    26821 agtgcgtgcg ggttcttgat aaaccgggca ttacgccaga tggtttaaca ttagtgggat
    26881 ttgccatcgg cgtgctggct ctgccgtttc tggcgctggg ctggtatctg gcggcgctag
    26941 tcgtcatttt gttgaacagg ctgcttgatg gtctggacgg tgcgctggcg cggcgcagag
    27001 ggcttaccga cgcgggcggc tttcttgata tttctctcga ttttctcttt tacgcgctgg
    27061 tgccgtttgg ctttattctt gctgcaccgg agcaaaatgc gctggcaggc ggctggttgt
    27121 tgtttgcgtt tatcggcacg ggcagcagtt ttctcgcctt tgctgcactg gcagcaaaac
    27181 atcagattga taaccccggt tatgcacata agtcgtttta ctatctgggg ggattaaccg
    27241 aaggcacaga aacgatctta ctgtttgtgc tgggatgttt attccctgca tggtttgcgt
    27301 ggtttgcgtg gatcttcggt gcgttgtgct ggatgacgac ctttacgcgg gtgtggagtg
    27361 ggtatctgac gctgaagtcg ctccagcgtc agtaattgca atgttatttg ctactgcccg
    27421 ggccgcgttc accggttgca accggatttt ttggatcgct gctccattcg taccagccgc
    27481 cgtcatacac ggaaacattc ttccaaccca tggcgcgtgc gtacataaag gtttcggacg
    27541 cgcgccagcc ggtgccgcag tagaatgaaa cttgctgatc tggtttgata ttccatgctt
    27601 tccacatagc ggtaatatca tcggcgctac gcatggtgcc atccgggtta tagaaatctt
    27661 ccatatgcgt cgagtcgcta ccagcgtgtc cccaacgtgc tccggctatt tcaccttttg
    27721 gtttaatata gctgtaaccg ctggtcgtac cgataaattc tggccacgaa cgaatgctca
    27781 ccagcgatgc atcctggcga tgcagcagtc cacgggcctg ttccatatcg agcatcaact
    27841 gcggttgtgc cgggatcttc acgccgaaat ccggttccgc tttcactttc ggtggcgttc
    27901 ctcgctcaac aggcagtccc gcgtcggacc aggtttgcca gccgccatcc agcagacgca
    27961 catctttcac gcctgcgtag agcataattt gcgccacacg cgctccagcg tatacgtcac
    28021 gcccgtacag aatgaccgtg gtgtcatggc gaatgccgtg ttttgccagc atcgctttta
    28081 gttgttcatc agaaactttg ttccacagcg gttcgctttc cacttcgttg gtatcgatgt
    28141 agtcagcgcc aggaatatgg ctgataaggt aaagcttagg agcgccctag gccgcttcaa
    28201 tgactttcca gtcaccgaca ggtttcgccg taacctcttt accttgttgc aggtcatgca
    28261 gccattgcgg ataaaccagc tgctcaaagt gcggcagttt ttgtagacgg gaaggttcgc
    28321 ttagcgcgtc actcagaatg gagatatgcg taaaacctgc tttttgcagt cgcgttttga
    28381 cggcgtcgac atctttgcca ttaccgtaca gcgccaccgg ggcatcggct ttcaggttat
    28441 gttgcttgat ccacgcgttg agctgttcgg tgctcatttt gtcgagccag ctagcagaga
    28501 ggtttaaggc ggcaggttca tgaccagaag ggccatttaa ggtttgtggc cagccgttat
    28561 aaaacgcgct ggggcgagta tctatcgctt tgccattttg ttgctgaagt tggtcaagtg
    28621 taagaggctt cgccagttca gcggcccacg aagaagcgca agccagccct aaagccattg
    28681 ccagcgcggt catttgagaa acacgtttca tcgaaaatcc cgacattgta gttttgagtg
    28741 cgccattctg aactgacgca ttgagaaaaa ctttgcgtta tcgcagtttg ttgtaatttt
    28801 ctgaccactg cgccatatcc agaacagaac tatcagcagg aacatcctgg agatcgtgcg
    28861 ttacctgaac gacggggatc gccagggcgc gaacttcgct gaacacccac tggcgaaaat
    28921 tatcgcgcag agccacatca agacggctga atggctcatc caggagtaac gcttttggct
    28981 gggcgagaag ggcgcgtagt agagcaacgc gcgctcgctg accgccagac aaagtggcag
    29041 gatcctgatg gaaagctccg tcaaggcctg aacgctcaag tgcatcattc acggcattac
    29101 gtcgggcatt ccccttaagt gtcgccggta gcgccagcag taaattttgc ccgacgctga
    29161 actggtcaaa taacagtgca tcctgaaaaa gaatgccaat ctgacgctgt gcggtgggta
    29221 gcatgtcaat ccgttgctca ttgagccata gctcacctgt acaagaaaac tgtccggcca
    29281 gtgcaccaat catccatgaa aacagagtgg attttccaca gccagacggc cccattaacg
    29341 tgacgatgtc acctttatca accgtaaagt taacgtttgt cagcaagcgg ctttctggta
    29401 aacgtagcga aacatttttc acgcagagca ttagcggatt ccttgtctga cataacctac
    29461 ccattttgcg actaacgcgg tcagggcaaa aataataagc ggtaacaaca gttgccataa
    29521 cgcctgggcg gcgagaatac cgttgctgcc gccgctgctt aatgccaccg cctcagtggt
    29581 gagcgtcgga aaacgccccg cgcccagcca cagcgtcggc atatactgcg caatactgac
    29641 tgaaaatccc accgcgaagg cagtcagcac agggcgcaac atgagtgggc atttcacgta
    29701 aaagaagatt ttggcccgcg accagcccag tgtttgcgca atcaaaatta accgtgaatc
    29761 aatgcgctgc caggcgggtt gcaggataaa cagcatccac ggcattaccc acagcagatg
    29821 cccccagacc accgccgtcc agcttccatc caggttcagc catagcgcca gcgtgtactg
    29881 gcccgccacc agtggcagag cgggcaatag aatgggcaac catagccaca actggcgacg
    29941 ctgtgccccc cattccagcc acagcagtaa caggagcaaa gcgatgaatg ttgctaccag
    30001 ccccattgtc agactgttga tgagtgcttc actattgatc gtcgactgat ccgcgaggat
    30061 cgccagcaga accacacaca gcacaccggt taagggtaaa aaactcgcca gcgtagtgcc
    30121 tggtaataaa ggtgtggcag gcttgcgaat gccatctacg cggggaatag tgcgccgcca
    30181 gctgcgccaa aacgtaggcg gcgagtaaca gcatcagcag cagactggcg agtgcgcctt
    30241 tcgtttgttg atccgcgtcg ccctgagtta accactgcca gctaattact gccagcgtcg
    30301 gtggattacc tggcccaaga ataatcgcca catccacgac cgaaagcgac caggcaacaa
    30361 tcgccagcat cgccattgcc agcgaaggcg cgacggaggg caacagcagc cagttcaggc
    30421 attgccagcg gctgtagccc agcgaatcca gcacaatgac ctgctgcaac agccgttttt
    30481 cgctcaatac tgccactaag atccacagca gaaatgcgct ttctttcact gcaagggtga
    30541 ggcccagacc gatccaaatc ggtccattgg cggagtgaaa tacgggaaat agtcatatag
    30601 cagccctccg tcagcaaaga gcagcagggc gctggtggca aaagccacat ggggaatggc
    30661 gagcagccac ggcagacggg cgcacatacg ctgccatttc ggcccaggcc atagcgccac
    30721 aatcaccagc agggcaatca acaatgcccc gaccgccgcg atggttgtcg acaccagcgt
    30781 tgccagtaat gcctgcggta actgtggatc ggcaaataac gcctgccagc gtgtcaacga
    30841 caacgcaggc gatatcagcg tgagagctgc cgggatcagc ggtgcataaa tcaccgccac
    30901 catcgcccac agcaaaaaga ttaatgcgta ccgtaacggc gtaaccattc ttgttccagt
    30961 gcattcaccc atcctgcgtg cggttcagcc agtaccggcg gcagatcctg cggcattctt
    31021 gattgcaata tttcgcgctg cccgtcaggc agtttttgcg gatcgagaac agaaggatcg
    31081 ccccagacaa cgggatctgc tttacgcagt tgcgcatcgg gtgagagcag gaaattggca
    31141 actaccttcg ccgcagcact ggcattcgcg ttggctggag tggtgacgaa atgcacgttg
    31201 ccaatcatcc cctcgcgaaa gccaaaactg taactgcttg ccggcaaatc gccgctggcg
    31261 attttttgct gcgcatgtgc ggggttaaag gtcagcgaca ggcgcaatgt gccggctttc
    31321 agcagagcat ccatccgcgc gggcgaaggc gggaaatcct ttccttcgcg ccacaaatac
    31381 ggatgcagtg catcaagata ttgctacaag ggcgcagtga cacgggcgaa agtcgcatcg
    31441 tccggcgctt cttttaatgc ggcgggatct ggcgtcagca taatcagcaa ctgttcaaga
    31501 aacgccgtgc cggtaaagtc cggtgggcgc ggataagtaa ccgtgccggg attggcttta
    31561 gcaaactcca gtaaggcttg cggcgtttgt ggtggctgtg gcgtaacatc gcggcgggcg
    31621 ataaacgtca gttgtgcgcc gccccagggc gattccgcac cttgtgtcgg cactgaaaaa
    31681 tctccccgca ccggcagctg tgtgtcgaca tagcgccagt tgggcagagt ctccgcccag
    31741 cccgtttgta ataaattggc ctcttt
//
LOCUS       DAEEUE010000042        29802 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.042, whole genome shotgun sequence.
ACCESSION   DAEEUE010000042 DAEEUE010000000
VERSION     DAEEUE010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29802
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.042"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            58..852
                     /locus_tag="KLK84_002047"
     CDS             58..852
                     /locus_tag="KLK84_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBE2009370.1"
                     /translation="MKIFKYTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLY
                     DAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETW
                     FNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDI
                     TMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVH
                     HHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            874..2283
                     /locus_tag="KLK84_002048"
     CDS             874..2283
                     /locus_tag="KLK84_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE2009371.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2261..3040
                     /locus_tag="KLK84_002049"
     CDS             2261..3040
                     /locus_tag="KLK84_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBE2009372.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3037..3897
                     /locus_tag="KLK84_002050"
     CDS             3037..3897
                     /locus_tag="KLK84_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBE2009373.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4045..4620)
                     /locus_tag="KLK84_002051"
                     /pseudo
     CDS             complement(4045..4620)
                     /locus_tag="KLK84_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4637..4897)
                     /locus_tag="KLK84_002052"
     CDS             complement(4637..4897)
                     /locus_tag="KLK84_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HBE2009374.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4888..6159)
                     /locus_tag="KLK84_002053"
     CDS             complement(4888..6159)
                     /locus_tag="KLK84_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBE2009375.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6237..6599)
                     /gene="queD"
                     /locus_tag="KLK84_002054"
     CDS             complement(6237..6599)
                     /gene="queD"
                     /locus_tag="KLK84_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HBE2009376.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6918..8717
                     /gene="cysJ"
                     /locus_tag="KLK84_002055"
     CDS             6918..8717
                     /gene="cysJ"
                     /locus_tag="KLK84_002055"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HBE2009377.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8717..10429
                     /gene="cysI"
                     /locus_tag="KLK84_002056"
     CDS             8717..10429
                     /gene="cysI"
                     /locus_tag="KLK84_002056"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HBE2009378.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10503..11237
                     /gene="cysH"
                     /locus_tag="KLK84_002057"
     CDS             10503..11237
                     /gene="cysH"
                     /locus_tag="KLK84_002057"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HBE2009379.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11501..11653
                     /locus_tag="KLK84_002058"
     CDS             11501..11653
                     /locus_tag="KLK84_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBE2009380.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11847..12938
                     /locus_tag="KLK84_002059"
                     /pseudo
     CDS             11847..12938
                     /locus_tag="KLK84_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(13084..14121)
                     /gene="iap"
                     /locus_tag="KLK84_002060"
     CDS             complement(13084..14121)
                     /gene="iap"
                     /locus_tag="KLK84_002060"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HBE2009381.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14373..15281
                     /gene="cysD"
                     /locus_tag="KLK84_002061"
     CDS             14373..15281
                     /gene="cysD"
                     /locus_tag="KLK84_002061"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HBE2009382.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15283..16710
                     /gene="cysN"
                     /locus_tag="KLK84_002062"
     CDS             15283..16710
                     /gene="cysN"
                     /locus_tag="KLK84_002062"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HBE2009383.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPDNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16710..17315
                     /gene="cysC"
                     /locus_tag="KLK84_002063"
     CDS             16710..17315
                     /gene="cysC"
                     /locus_tag="KLK84_002063"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HBE2009384.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            17365..17688
                     /locus_tag="KLK84_002064"
     CDS             17365..17688
                     /locus_tag="KLK84_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HBE2009385.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGSLF
                     MWLLG"
     gene            17882..18193
                     /gene="ftsB"
                     /locus_tag="KLK84_002065"
     CDS             17882..18193
                     /gene="ftsB"
                     /locus_tag="KLK84_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HBE2009386.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            18212..18922
                     /gene="ispD"
                     /locus_tag="KLK84_002066"
     CDS             18212..18922
                     /gene="ispD"
                     /locus_tag="KLK84_002066"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBE2009387.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18922..19401
                     /gene="ispF"
                     /locus_tag="KLK84_002067"
     CDS             18922..19401
                     /gene="ispF"
                     /locus_tag="KLK84_002067"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBE2009388.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19398..20447
                     /gene="truD"
                     /locus_tag="KLK84_002068"
     CDS             19398..20447
                     /gene="truD"
                     /locus_tag="KLK84_002068"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HBE2009389.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20428..21189
                     /gene="surE"
                     /locus_tag="KLK84_002069"
     CDS             20428..21189
                     /gene="surE"
                     /locus_tag="KLK84_002069"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HBE2009390.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            21183..21809
                     /gene="pcm"
                     /locus_tag="KLK84_002070"
     CDS             21183..21809
                     /gene="pcm"
                     /locus_tag="KLK84_002070"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HBE2009391.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21949..23088
                     /gene="nlpD"
                     /locus_tag="KLK84_002071"
     CDS             21949..23088
                     /gene="nlpD"
                     /locus_tag="KLK84_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HBE2009392.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTMSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            23151..24143
                     /gene="rpoS"
                     /locus_tag="KLK84_002072"
     CDS             23151..24143
                     /gene="rpoS"
                     /locus_tag="KLK84_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HBE2009393.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(24263..24670)
                     /locus_tag="KLK84_002073"
     CDS             complement(24263..24670)
                     /locus_tag="KLK84_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBE2009394.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24817..25410
                     /locus_tag="KLK84_002074"
     CDS             24817..25410
                     /locus_tag="KLK84_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBE2009395.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            25410..26357
                     /locus_tag="KLK84_002075"
                     /pseudo
     CDS             25410..26357
                     /locus_tag="KLK84_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26630..27193)
                     /locus_tag="KLK84_002076"
     CDS             complement(26630..27193)
                     /locus_tag="KLK84_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009396.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            27358..27609
                     /locus_tag="KLK84_002077"
     CDS             27358..27609
                     /locus_tag="KLK84_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE2009397.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27611..29707
                     /locus_tag="KLK84_002078"
     CDS             27611..29707
                     /locus_tag="KLK84_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE2009398.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         7301 a         7382 c         8059 g         7060 t
ORIGIN      
        1 attcgtcaca tcatcatcgg caacgaaggt gcattgtgct atatcactga agtaacagtg
       61 aaaatcttta aatacacccc ggaaaacaac ctcttctacg gctatatcct ggaagacatg
      121 aaaaccggct tcaacatcct gcgtgaaatc atggtggaag ggtatcgtcc gtcgatcgct
      181 cgtttgtatg acgctgaaga tggcacccaa cacttcaccc attttgccga cggaaaatgt
      241 gtgctgatct ttatggctga aggtaaccct cgcattgcga aggcgacggg cgaagggatt
      301 gcggaaatcg ttgcccgcta cccgcagtgc cagcgcgtgg acagcaaact gattgaaacc
      361 tggtttaaca acctgaactg gggaccggat aaagtggctg ccgaacgtgt gcagatcctc
      421 aaaaccggca acatgggctt taccaccgaa gtgtccggct gctggagctg catccacgaa
      481 atctacgaaa gcgttattaa ccgtattcgt actgagttcc cgcacgccga cgacatcacc
      541 atgctgggcg gtcactcctc tcatagctat cagaacggca ccaacatgta cttcgtctat
      601 gactacaacg ttgttgactg taagccggaa gaggaaatcg acaagtacca caatccgctc
      661 aacaaaatca tctgcgaaga aaccattcgc ctcggcggtt cgatggtgca ccaccatggc
      721 attggtaaac atcgcgttca ctggagcaag ctggaacacg gcagcgcgtg ggcgttgctg
      781 gaagggctga aaaagcagtt cgatcctaac ggcattatga atacgggtac tatctatccg
      841 attgaaaaat aatgtatcag gcagcgttcc gcgatgacgg ggcgttgcct tttcggcttc
      901 tcaggcgaga agccgttctt attaccggac aatgaagggg taaagatgaa cacttcaccg
      961 gtgcgaatgg atgatttacc gcttaaccgt tttcactgtc gaattgctac gctcactttc
     1021 ggcgcacacc tgaccgacgg ttatgttcta ggcgtcattg gttacgccat tattcaactt
     1081 acgcccgcca tgcaactgac gccgtttatg gcgggaatga tcggcggctc ggcgctcctt
     1141 ggtttgtttc ttggcagcct ggttcttggg tggatctccg accatattgg tcggcaaaaa
     1201 atcttcacct tcagcttttt gctgattacg ctcgcttcgt tcttgcaatt ttttgccacc
     1261 acgccagagc atcttattgg gctgcgcatt ttgatcggca ttggtctggg aggcgattac
     1321 tcagtaggtc acaccttgct ggctgaattt tccccgcgcc gccatcgcgg tattttgctg
     1381 ggcgcattca gcgtggtgtg gaccgtaggc tatgtgttgg caagtattgc cggacatcac
     1441 tttatttccg aaaacccgga ggcctggcgc tggttgctgg catcggcagc tctgcccgcg
     1501 ttgttgatta cgttattacg ctggggaacg ccagaatcgc cacgttggct actgtgccag
     1561 gggcgttttg cagaagctca cgctatcgtg catcgctatt ttggtcccca tgttttactg
     1621 ggcgatgaag tggtaacggc gacccataaa cacatcaaaa ccttgttctc ttcgcgttac
     1681 tggcggcgca cggcgtttaa cagcgtcttc tttgtctgcc tcgtaatccc atggtttgtg
     1741 atttatacct ggctgccaac tatcgcccag actattggtc tggaagatgc gctgactgcc
     1801 agcctgatgc ttaatgcgtt gttaattgtg ggcgcgctgc tgggattagt tctgacgcac
     1861 ctgctggcac atcgcaaatt tttgctggga agttttttgc tgctggcggc aacgctggta
     1921 gtaatggcct gtttgccttc cggcagttca ttaacgctgc tgctttttgt tctcttcagc
     1981 accaccattt cggcagtcag taatctggtg ggcattttgc ctgcggaaag ttttcctact
     2041 gacattcgct cgctgggcgt cggttttgcc accgccatga gtcgacttgg cgcggcggta
     2101 agtactggcc tgctgccgtg ggtgctggcg cagtggggaa tgcaagtcac cttattgctc
     2161 ctagcgacag tgttgttggt tggttttgtt gtgacctggc tatgggcacc agaaactaaa
     2221 gccctcccgc tggtggcggc gggaaatgta ggaggtgcga atgaacattc tgttagcgtt
     2281 taaagccgaa ccggatgccg gaatgctggc ggaaaaagag tggcaggcgg cggctcaggg
     2341 taatagcgga ccggatgttt cgctactgcg aagtttactc ggtgctgatg aacaggccgc
     2401 tgccgcgctg ctgttagcgc agagaaaaaa cggtacgccg atgtctttaa ccgcgttgag
     2461 catgggggat gaacgggcgt tgcactggct gcgctatctc atggctcttg ggtttgagga
     2521 agctgtattg ctggagacgg cggcagatct gcgctttgcg ccggaatttg tcgctcgcca
     2581 tattgccgaa tggcagcgtc agaatccgct ggatctgata attaccggct gccaaagcag
     2641 cgaagggcag aatgggcaaa cgccattttt gctggcggaa atgctgggct ggccctgctt
     2701 cacccaggtg gagcgtttca ctctcgacgc gctgtttatc accctcgaac agcgtactga
     2761 acatgggttg cgctgttgcc gggttcgctt gcctgcggtg attgccgtgc gtcagtgcgg
     2821 tgaagtggcc ttgccagtac cgggaatgcg ccagcgaatg gctgcgggga aagcagaaat
     2881 tatccgtaaa actgttgctg ccgaaatgcc agcgatgcag tgtctgcaac tggcaagagc
     2941 agagcaacga cgcggagcga cgctgattga tgggcaaacg gtagcagaaa aagctcaaaa
     3001 gctatggcag gactatttac gccaaaggat gcagccatga atatcgcaat tgtgacaata
     3061 aatcaggaga atgccgcaat agcgagctgg ctggcggcgc aggatttcag cggttgtacg
     3121 ctggcgcact ggcaaataga acctcagccc gtagtggcgg aacaggtgct ggatgcgatg
     3181 gtggaacagt ggcagcgaac gcctgcggac gttgttctct ttccgccggg aacgtttggc
     3241 gatgaactgt cgacccggct ggcctggcgt ttacatggtg caagcatctg tcaggtgact
     3301 tcgttagata ttccaaccgt aagcatgcgc aaatcccact ggggaaatgc gttaacggcg
     3361 acgctgcaaa cagaaaaacg cccgttatgc ctttccctgg cgcgtcaggc gggagcagcg
     3421 aaaaacgcca cgttgccctc tggaatgcag caactgaaca ttgtcccagg tgcgcttccc
     3481 gactggctgg ttagcacaga ggatctgaaa aatgttagcc gcgacccatt agctgaggcc
     3541 agacgggtgc tggtggtggg acagggcgga gaggcagata accaggaaat cgttatgctg
     3601 gcagaaaagc tgggagccga agtgggctac agtcgggcgc gagtgatgaa cggtggcgtt
     3661 gatgctgaaa aagtgattgg tatttccgga catctgctgg cacctgaggt gtgcattgtt
     3721 gttggcgctt ccggcgcagc agcgttaatg gcgggcgtac gcaatagcaa gtttatggtg
     3781 gcgattaatc atgacgccag cgcagcggta ttttcacagg cagatattgg cgtggttgat
     3841 gactggaaag tggtgctgga ggcactggtg acaaacattc acgcagaata ccaataacgt
     3901 gctgccggat gatcctgcct acggttctgg actggggacc tgtaggcctg ataagacgcg
     3961 ttaagcgtcg catcaggcgc tgaatgtcgg atgtggcgtg cggccaacaa atcaaaacaa
     4021 attcaattgg ttacgattat cccgtcaaag caattttttc gctaacgtcc agactccggt
     4081 attcgtggtg gaaagcgcca cgacacccgc gttaatcgca ttacgcgcat cttcttcatc
     4141 gcataccagc ccgccagcaa tcagcggttg gcggattttt tccgtcaccc agcccagcac
     4201 tttgggcatg cagcctggca ggatctcaat acaatccgga ttcgattgcg caacttactt
     4261 atcaatgttg tgaaacgaaa tcgaatcaac aataaacagg tgatgaatac agaaaaaaac
     4321 ttccgctctt gccgctttta gcatcgatgc tttggtgctg ataattccat ccgcctcggt
     4381 caccagtttc aaaaactgaa tcaccacttc tttatttgac gcgccttcca gcaaatcaac
     4441 atgaataaaa gcatatttcc cggcgttctt aatcttttta acgatattac tgatggtgca
     4501 gatattgccg tacaacacgg aaataaactg gcattcagaa tcgatggcca gttgcaggct
     4561 ggcattatct ttaacggcag caatcaccgg attctggcgg agcaggtgta agaggggcat
     4621 aaattatcct tattatttat ccaaagcggt aggtgatgcc gaatccggaa ggcggatagc
     4681 gccactgttg tagtgtggat tcgtcgcaca gcaaacggca ggtgccacac tccaggcagc
     4741 cgcgatagtc aatacgtaag tcaccttcct gtgtgagaga aaaaagacct accggacagg
     4801 cgttaatcaa ccgttgtacc atctgacgct caacggagtc agccggaaca atgtgcggcg
     4861 catcagcaac gcgccagaga ttacgggcta cagacatcgc agactcctga taacatcgcc
     4921 cgccagatgc cacaggccat gacgacgtaa atgatgccag aataactggc gcagcggtgg
     4981 aacaggttta tcaccctgat cccataaatc gcgggaaata tcctgcatta acgcaggcca
     5041 cgcccgatac cagccagggc gttgcaaaag cgccggaaca tgctgataac gttgtagaac
     5101 atcccatagc aggctgcgct ctacgttgtg atgataaagc gcaaacagat tttgcggctc
     5161 gcggtgctgg caggcgctta ttagcgtttg tgccgccgcc tgtgcgccag tcagcgccat
     5221 atccatgccg cgcacggaaa ttccggtatt gacgcaactg cgcaacgcat cgcccaccag
     5281 cagccagccg ttaccggcgt attgcaccgg catactgtgc aagccacctt ctggcaccag
     5341 atgcgcacca tactccagtg attccgtgtt tttgataagc gtgcgcactg ccggatgcgc
     5401 tttaaagcga gtcagcagct cgcttgccgg aacacgactt tgcgtaaggg aagagagcgg
     5461 gcaaacaatc cctaacgaga gcgtttgttg attagtataa agaaatgcgc cgccgggtaa
     5521 gtcatcacag attccgccgc tgaacagcaa cgctgcgcct tcgttattct ccagatgaaa
     5581 acgttcttca atagcggatg tttccagcga cagcacttct ttgatcccca acgccatcgc
     5641 ttcgccagca ggacgagtca ctaacccgtg acgttcagcc aggacgctgt tggcaccttc
     5701 tgccagcacc acataacggg cgcggagaat atcgtcacca caaataacgc cacagactct
     5761 gccgttttct tcatacagtg catccaccgt cgctccgggg atgcattcga caccttcttt
     5821 ttcggcttcg gcaaccagcc acggatcgaa ccgtgcacgt aatacactcc aggattcacc
     5881 gccgggctgt aagctggaaa acgtcgttgc gccatccggc gttaacaggg aaaggctttc
     5941 gtgagtgatg cgtcgttcaa gaggcgcggt cagatgaaat tgcgggagga gttccgcgag
     6001 tgcatgggta tataaccgcc tgccggaaag atttttgctg ccggggattt cagcgcgttc
     6061 gagtaacaaa acggataaat ccgctcgcgc gcagcgtaac gcgcaagcgg tgcctgcaat
     6121 accggcacca ataataataa tgtcgcagtc gtcttccatg tcatgcccca gatatccaga
     6181 acacaacacc ctaacatagc gttactaaag ggaaattgac cgccgacaca ctcttatcat
     6241 tcgccgcgat agatacaccc tgcggtgcag gtttctttta ccatcaccgc acttaacagc
     6301 ggcacaacgg gtttaacctg atcccaaatc cattttgcta aaacttcgct ggttggattt
     6361 tccagacctg gaatatcatt gagataatgg tgatcgaggc gctcataagt tggcttaaac
     6421 gccgctttca gttcagcgaa atcgataatc cagcccgtat gcggatcgac ttccccggta
     6481 atttccagtc gcaccataaa ggaatgcccg tgcaggcgac cacatttatg cccttccggg
     6541 acatgtggta agcggtgagc ggcttcgaag gtgaaatctt taaataacgt ggtggacatc
     6601 ataatttctc tacaggacga taaaaccgcc gtagagtacc ggaaaggctg ttttttcgca
     6661 ttatctaaaa cgactttgtt gcgcaaaatc gctgatttat cttaatgatt ggctaaattc
     6721 atttgttttt cattaggttg gttaatctat tttgttgtta aagactattg ctaaaacagg
     6781 ttagtcgatt tggttattag ttatcgctat cccgtcttta atccacaccg ttcaccccgt
     6841 taaccttacc ttctcttctg ttttatgggc gctgacaggg cgcagaaaca gctttgctta
     6901 ctggaacata acgacgcatg acgacacagg tcccaccttc cgcgttgctt ccgttgaacc
     6961 cggagcaact ggcacgcctt caggcggcca cgaccgattt aacgcctacc cagcttgcct
     7021 gggtttctgg ctatttctgg ggcgtgctca accagcagcc tgttgcgctt gcagtgacgc
     7081 cagcgcccgc cgcagaaatg ccgggtataa cgattatctc tgcttcgcaa accggcaatg
     7141 cgcgccgggt tgctgaagca ttacgcgatg atttattagc agcaaaactg aacgttaagc
     7201 tggtgaacgc gggcgactat aaattcaaac aaatcgccag cgaaaaactg ctcatcgtag
     7261 tgacgtcaac gcaaggggaa ggggaaccgc cggaagaagc cgtcgcgctg cataagttcc
     7321 tgttctccaa aaaagcgcca aagctggaaa acaccgcgtt tgccgtgttt agcctcggcg
     7381 atagctctta tgaatttttc tgccagtccg ggaaagattt tgacagcaag ctggcggaac
     7441 tggggggtga acgcctgctc gaccgtgtcg attctgatgt tgaataccag gctgctgcca
     7501 gcgagtggcg cgcccgcgtg gttgatgcgc ttaaatcgcg tgcgcctgtc gcggcacctt
     7561 cgcaatccgt cgctactggc gcggtaaatg aaatccacac cagcccgtac agcaaagacg
     7621 cgccgctggt ggctagcctc tctgttaacc agaaaattac tgggcgtaac tctgaaaaag
     7681 acgttcgcca tatcgaaatt gacttaggcg actcgggcct gcgttaccag ccgggtgacg
     7741 cgctgggcgt ctggtatcag aacgatccgg cactggtgaa agaacttgtc gaactgctgt
     7801 ggctgacagg cgatgaacct gtcactgtcg agggcaaaac gttgcctctg aacgaagcgc
     7861 tacagtggca cttcgaactg accgtcaaca ccgccaacat tgttgagaat tacgccacgc
     7921 ttacccgcag tgaaacactg ctgccgctgg tgggcgataa agcgaagtta cagcattacg
     7981 ccgcgacgac gctgattgtt gacatggtgc gtttctcccc ggcacagctt gatgccgaag
     8041 cgctaattaa tctgctgcgc ccgctgaccc cgcgtctcta ttccattgcc tcctcgcagg
     8101 cggaagtcga gaacgaagta cacgtcaccg ttggtgtggt gcgttacgac gtggaaggcc
     8161 gcgcccgtgc cggtggtgcc tccagcttcc tcgctgaccg cgtggaagaa gagggcgaag
     8221 tccgcgtatt tatcgaacat aacgataact tccgcctgcc cgctaacctg gaaaccccgg
     8281 tgattatgat tggcccaggc accggtattg cgccgttccg cgcctttatg cagcaacgcg
     8341 ccgctgacga agcgccaggt aaaaactggc tgttctttgg caatccgcat tttacggaag
     8401 acttcctgta tcaggtggag tggcagcgct acgtcaaaga gggtgtgctg acgcggatcg
     8461 atctcgcctg gtcgcgcgac caaaaagaaa aagtttacgt acaagataaa ctgcgcgaac
     8521 agggcgcgga gctgtggcgc tggatcaatg acggtgccca catttatgtc tgcggcgacg
     8581 ctaatcgcat ggcgaaagac gttgagcagg cacttctgga agtgattgcc gaatttggtg
     8641 gcatggacac cgaagcggcg gatgaatttt taagtgagct gcgcgtagag cgccgttatc
     8701 agcgagatgt ctactaatga gcgaaaaaca tccagggcct ttagtggtcg aaggaaaact
     8761 gacagacgcc gagcgcatga agcttgaaag caactacctg cgcggcacca ttgcggaaga
     8821 tttaaacgac ggtctgaccg gcggctttaa gggcgataac ttcctgctga tccgcttcca
     8881 cggcatgtat cagcaggatg accgcgacat ccgcgccgaa cgtgctgaac agaagctgga
     8941 gccgcgccac gcgatgctgt tgcgctgccg tctgccgggt ggagttatca ccaccaaaca
     9001 gtggcaggcg atcgataaat ttgccggtga aaacaccatc tatggcagca ttcgcctgac
     9061 caaccgccag acgtttcagt tccacggcat tctgaaaaag aacgtcaaac cggtgcacca
     9121 gatgctgcac tcggtcggtc ttgatgcgct ggcgactgct aacgacatga accgtaacgt
     9181 actctgcacc tcgaaccctt acgagtcgca gctgcacgca gaagcgtacg agtgggcgaa
     9241 gaagatttct gaacatctgc tgccgcgtac ccgcgcgtat gcggagatct ggctcgatca
     9301 ggaaaaagtc gccaccaccg atgaagaacc gatccttggt cagacttacc tgccgcgtaa
     9361 gttcaaaacc acggtagtga tcccgccgca gaacgatatt gatctgcacg ccaacgacat
     9421 gaacttcgtg gcgatcgcgg aaaacggcaa gctggtgggc ttcaacctgc tggtgggcgg
     9481 tgggctttct atcgaacacg gtaacaagaa aacctacgcc cgcacggcga gtgagtttgg
     9541 ctatctgccg ctagagcata cgctagcggt ggcggaagcc gtcgtgacaa ctcagcgtga
     9601 ctggggtaac cgaaccgatc gtaaaaatgc caaaaccaaa tacacactgg agcgcgtggg
     9661 ggttgagacg tttaaagcgg aagtggagcg tcgcgcgggg atcaaatttg aaccgatccg
     9721 cccgtatgaa tttacaggac gcggcgatcg tattggctgg gttaagggca ttgatgataa
     9781 ctggcacctg acgctgttta tcgaaaatgg tcgcatcctt gattatccgg ggcgtccgct
     9841 gaaaactggc ctgctggaga tcgcgaagat ccacaaaggt gatttccgca ttacggcgaa
     9901 ccagaatctg atcatcgccg gtgtgccgga aagcgagaaa gcgaagatcg agaaaattgc
     9961 caaagagagc gggttaatga atgccgtcac gccgcagcgt gaaaactcga tggcctgcgt
    10021 gtcattcccg acttgcccgc tggcgatggc ggaagcggag cgtttcctgc cgtcttttat
    10081 cgacaacatc gataatttaa tggtgaaaca tggtgtcagc gatgagcata tcgtgatgcg
    10141 tgtaacaggc tgcccgaacg gttgtggtcg cgcgatgctg gcggaagtgg gcttggtggg
    10201 taaagcaccg ggtcgctaca acctgcatct tggcggcaac cgcattggga cacgtatccc
    10261 acggatgtat aaagaaaaca tcaccgagcc ggaaatcctg gcgtcgcttg atgaactgat
    10321 agggcgctgg gcgaaagagc gcgaagcggg tgaaggcttc ggcgacttta cggtgcgtgc
    10381 gggcatcatt cgcccggtgc tcgatccggc gcgcgatttg tgggattaac catcagcccg
    10441 gtcttgtagg cctgataaga cgcgtgagcg tcgcatcagg caaggcaaac agtgaggaat
    10501 ctatgtccaa actcgatcta aacgccctga acgaactgcc gaaggtagat cgcattctgg
    10561 cgctggcaga aactaacgcc gaactggaaa aactggacgc tgaaggccgc gttgcctggg
    10621 cgctggataa tctgcccggt gaatatgtac tttcttccag ctttggcatt caggcggcgg
    10681 tgagcctgca tctggtgaat cagattcacc cggatattcc ggtgatcctc accgacaccg
    10741 gctatctgtt cccggaaacc taccgcttta ttgacgagtt aacggacaaa ctcaagctca
    10801 acctgaaagt gtaccgcgct accgaaagcg ccgcctggca ggaagcacgc tacggcaaac
    10861 tgtgggaaca gggcgttgaa ggcattgaaa agtacaatga catcaacaaa gtcgaaccga
    10921 tgaaccgggc gctgaaagaa ctgaatgcgc aaacgtggtt tgctggcctg cggcgtgaac
    10981 aatccggcag ccgtgccaat ttaccggtgc tggcaattca gcgtggcgta tttaaagtgc
    11041 tgccgattat cgactgggat aaccgaacta tttatcagta cctgcaaaaa catggcctga
    11101 aatatcaccc attatgggac gaaggatatt tatcggtggg cgatacccat acaacccgta
    11161 aatgggaacc cggcatggcg gaagaagaaa cgcgtttctt tggcttaaaa agggaatgtg
    11221 gattacacga agggtaaata ttgccggata aattaaacgc ctctgtcaga aatgatgggg
    11281 gcgtttttat tttgcgagca gcattccggc atttaaaggt gaacatcgca gggaaaatta
    11341 acaggtatag aatggcgttg ggtgcttgag gctgtctggc gttaagcatt cgcgaggttc
    11401 cagatggaca aaagccccag gcgatatttc tatcaacctg aggctcgcgt tcgaacgtag
    11461 caattcggat gttagtctct tctttgcagg gaggcaagac atgctgacaa aatatgccct
    11521 tgtggcaatc atcgtactgt gttgtacagt actgggattc acgttgatgg taggtgactc
    11581 gttatgtgag ttgagtatca gagaacgtgg tatggagttt aaggcagttc tcgcttacga
    11641 atcgaagaag tagccaccac gcgggggtaa aaccccgcgt actgaattgt gatggtttgg
    11701 tctcctgcac ccttttttta aatttatatt ttttatgtga atatatagtc tctatttatt
    11761 attatgaatt tcaaatgaaa atcattaatt tattcataat gtaaatgttt tattggctga
    11821 tatatatcat agatagtttt taaaatatgc gtaaatatcc tttaagttta ctgaaggata
    11881 aaaatattgt gactttcttt gatttctggg gaaaaaaccg acgtggcgag aaagagggta
    11941 tcgacgacta tcaccttctt tgctggcatt tgctggatgt ggccgcaatg ggctatttaa
    12001 tggttaaaag aaattgcttc gggctggctg attattttcg tcaattaggg atttctgaca
    12061 aggaacaggc agctcaattt ttcgcctggt tgctgtgctg gcacgatatt ggaaaatttg
    12121 cccgctcttt tcaacaactt tacctgcccc ctgaattcaa gattcaggaa ggtgccagaa
    12181 aaaattacga aaaaatctct cattcaacgc tgggctactg gttgtggaat cattatttaa
    12241 gtgaatgtca ggagttactt ccttcatctt cgctctctcc gcgcaaactt aggcgagtga
    12301 tagaaatgtg gatgcccgtg acgacagggc atcatggtcg gccccctgat cgtatggatg
    12361 aattggataa ttttctgcct gaagacaaag ctgcagcacg agattttctc cttgaaatca
    12421 agccactgtt tccgcgaata gagatcccca cattctggga tgatgacgag ggcgttgaac
    12481 ttataaaaca actttcctgg tatatctctg caaccgtcgt acttgcagac tggacagggt
    12541 cgtcaacgcg attttttcca cgcgtcgcac aggcaatgga tattgaagat tactggcaaa
    12601 aggctttagt tcaggctcaa aacgcattaa ccgtctttcc gccacaagca gaaaccgcgc
    12661 ctttcaccgg aattaatacg ctgtttcctt ttattgagaa tacgacaccg ttacagcaaa
    12721 aggtgctgga tctggatatc aatcagccag gaccacagtt gtttattctg gaagatgtga
    12781 ccggcgcagg taaaacagaa gcggcgctta tcctggcgca caggttgatg gctgcgggta
    12841 acgctcaggg gctgtttttt ggcctgccaa caatggcaac ggccaatgcc atgtacgatc
    12901 gactggtcaa aacctggctt gctttctatt cgccagaggt gttccccgct ccagcggaga
    12961 taaaccggcg caatacggtg cggttttccc ggaagtaaac cggacgcact ggatgcgatg
    13021 atgggtatca cttagaattc cccgcccctg cggtagaact cccagctccc attttcaaac
    13081 ccatcaagac gccttcgcca gctccttcac caacggtagc attatccgca taacgtcacg
    13141 gcagcgacgt tctattcttc caggaagtgc cttatcaata tgttgttgat tatccagtct
    13201 tacgtcatgc cagctatttc ccgccgggaa ggcaggtgtt tttgcgcgtt gctgataacc
    13261 atccttattt ccaagattcc agttagtcgc ttccaccgaa agtacagcaa tgcccgcttt
    13321 gtcgaatatt tctgcgtcat tacaacaccc agtgcctttc ggataatttt tattcaaacc
    13381 cggattggtc gttgcggcta ttccgtgact gcgtgcaatt gccagcgccc tgtcgcgcgt
    13441 taatttcctt actgcttcag gggtttttac accgctgttg aaatacaatt tatcgccaac
    13501 aattaagtta tcgagattaa tcaccagcag cgtatttttc ttttcggtgt cactcatccg
    13561 cttgagtaaa ttctcagcgc ctaattttcc ttcctcttcg ccgctggtcg ccacaaatcg
    13621 aataccatac tcggtaggcg tatttttcag gcgttctgcc agttccagca tgacacctaa
    13681 acctgcggcg ttatcatcta ttccttgtaa cgtcagtccg ccgagattgg catctgcgtc
    13741 agcatcgctc agcggggcgt aagtatccag atgcgccata atgatgatct gctgcggcgc
    13801 tttgccttca tgagcggcaa tcaccgtact tcctgtcacg ttatgccagt tcttgcgatt
    13861 atcgcgggcg gtataaatat accgactatt aaatgtccga atatcactgc gataacccat
    13921 ttgctgaaac tgttggcgaa tataatcggc agataacatt tctgctggag ttccggtcat
    13981 gcgtcccgga aagaaagtag caatatgtcg tgcctgggta ttagcaaaat cgccaggttt
    14041 aggtgacgag gcgtgtacgg ggagaataaa gcatacgccg agcgccaggg cagcggtacg
    14101 gtggcgcaat gcggaaaaca tagtgagtcc ttaaatacca tgcaaatttt tttaccgcca
    14161 tagtatgaaa ctgccgctgc gctaaaacaa tttcaaatct tcctaaacgc ccgaaatccg
    14221 gtgccttaag cactttttga tattagcttt gccaaatcgt tattccgtta aggaactact
    14281 cattctaatt ggtaatttca ttcgttctct tacgctccct atagtcgaaa catctgatgg
    14341 caagaaaata gcggtattgc aaaggaacgg ttatggatca aaaacgactt actcacctgc
    14401 ggcaactgga ggcggaaagc atccacatta ttcgcgaggt ggcggcagaa ttctcaaatc
    14461 cggtgatgct ctactctatc ggtaaagatt ccagcgtcat gctgcatctg gcgcgcaagg
    14521 cgttttatcc aggtacgctg cctttcccgt tgctgcatgt cgataccggc tggaaattcc
    14581 gcgagatgta tgagttccgc gatcgtacgg cgaaagccta cggctgcgag ctgctggtgc
    14641 ataaaaaccc ggaaggcgtg gcgatgggga ttaatccatt cgtgcacggc agcgcgaaac
    14701 ataccgatat tatgaaaact gaaggcctga aacaggcgct gaacaaatac ggttttgatg
    14761 ccgccttcgg tggtgcgcgc cgtgacgaag agaaatcccg cgctaaagag cgaatctact
    14821 ctttccgtga ccgcttccat cgctgggatc cgaaaaatca gcgcccggag ctgtggcaca
    14881 actacaacgg gcaaattaac aaaggcgaaa gcatccgcgt cttcccgctc tctaactgga
    14941 ccgagcagga tatctggcaa tacatctggc tggaaaatat cgacattgtg ccgctgtatc
    15001 tcgctgcgga acgtccggtt ctggaacgcg acggtatgtt gatgatgatt gatgacaacc
    15061 gtatcgacct gcaaccgggc gaagtgatca aaaaacggat ggtgcgtttc cgtacgctgg
    15121 gctgctggcc gctgaccggt gcggtggagt caaatgcaca aacactgccg gaaatcatcg
    15181 aagagatgct ggtttccacc accagtgaac gtcaggggcg cgtgattgac cgcgaccagg
    15241 cgggatccat ggagctgaaa aaacgtcagg ggtattttta agatgaacac cgtacttgca
    15301 caacaaatcg ccaatgaagg cggcgtcgaa gcctggatga ttgcgcaaca acataaaagc
    15361 ctgctgcgtt ttctgacctg tggtagcgtc gatgacggca aaagtaccct gattggtcgt
    15421 ctgctgcacg atacccgcca aatctacgaa gatcagctct catcgctgca taacgacagt
    15481 aagcgtcacg gcacccaggg cgaaaagctg gatctggctc tgctggtgga cggcctgcaa
    15541 gctgagcgcg aacagggcat cactattgat gtggcctacc gctatttctc taccgagaag
    15601 cgtaaattta ttatcgccga caccccaggg cacgagcagt acactcgcaa tatggcgact
    15661 ggcgcatcga catgtgaact ggcgatctta ctgattgatg cccgtaaagg cgtgctcgat
    15721 caaacccgtc gtcacagttt tatctccaca ctgttgggga tcaaacatct ggtcgtggcg
    15781 atcaacaaaa tggatctggt ggattacagt gaagagacgt tcacccgtat tcgtgaagat
    15841 tatctgacct ttgccgggca gctgccggat aatctggata tccgctttgt gccgctctcc
    15901 gcactggaag gtgacaacgt ggcttcgcaa agtgaaagta tgccgtggta cagcggtctg
    15961 acactgctcg aagtgctgga aaccgtggag atccagcgag tggtggatgc ccagccaatg
    16021 cgcttcccgg tgcagtacgt taaccgccca aatctcgatt ttcgtggcta cgccggaatg
    16081 ctggcatccg gtcgcgtgga agtcgggcaa cgtgtaaaag tgctgccctc tggtgtggaa
    16141 tcaaacgtcg cgcggatcgt gacttttgat ggtgatcgcg aagaagtctt tgccggagaa
    16201 gcgatcaccc tggtgctgac ggatgagatc gatatcagcc gtggtgatct gctgctggcg
    16261 gcagacgaag cgttaccggc ggtgcagagt gcgtcggtgg atgtggtatg gatggcggaa
    16321 cagccgcttt ccccgggcca gagttatgac atcaaaattg ccggtaagaa gacgcgtgct
    16381 cgtgttgatg gcattcgtta tcaggttgat attaataacc ttacccaacg cgaagttgaa
    16441 aacctgccgc tgaacggcat cggcctggtg gatctcactt ttgacgagcc actggtgtta
    16501 gatcgctatc agcaaaatcc ggttaccggt gggctgattt ttatcgatcg cctgagcaat
    16561 gtgaccgtgg gtgccggtat ggtgcacgag ccagttagcc aggcaacagc tgcgtcatct
    16621 gaattcagtg cattcgaact ggaattgaat gccctggttc gtcgccactt tccgcactgg
    16681 ggcgcgcgcg atttgctggg ggataaataa tggcgctgca tgacgaaaac gtcgtctggc
    16741 atagtcatcc ggtcactgtg caacaacgcg aactacacca cggtcatcgt ggtgtagtgc
    16801 tgtggtttac cggcctctcc gggtccggta aatcaacggt cgccggggcg ctggaggagg
    16861 cgttacataa actcagcgtc agtacgtatc tgctggatgg cgacaatgtt cgccacggat
    16921 tatgcagcga tctcggtttt agcgatgccg atcgtaaaga gaatatccgt cgcgtcggtg
    16981 aagtggcgaa tttgatggtt gaagccggac tggtggtgct gaccgcattt atctcgccac
    17041 accgcgccga acgccagatg gttcgcgaac gcgtaggaga agggcgcttt attgaagtgt
    17101 ttgtcgatac gccgctggcg atttgcgaag cccgcgatcc aaaaggctta tataagaaag
    17161 cgcgtgccgg tgaactgcgc aactttacgg gaatagattc cgtttacgaa gcgcctgaat
    17221 cggcagaaat tcatctcaat ggtgaacaat tagtaacaaa tttggtacag caattattag
    17281 atctgttgag acagaacgat attatcagat cctgagacac caccgggttt ccatgcccgg
    17341 ttcgtcaaag taacaggatc aaatatgcgt aatagccata acattacact aacaaataac
    17401 gacagcctta ctgaggatga agaaactaca tggtcactgc ctggtgccgt ggtcggtttt
    17461 atctcctggt tatttgcgct ggcgatgccg atgttgattt atggctctaa cacgctgttc
    17521 ttctttctct acacctggcc tttctttctg gcactgatgc ctgtcgcggt agtggtgggg
    17581 attgcgctgc attcattgat ggacggaaag ctacgctaca gtattgtttt caccctggtg
    17641 acagtaggca ttatgtttgg ctcactgttt atgtggctac tgggctaatt tgtactttct
    17701 tgtctcctct gttcataatt caaaccgtaa ctaataatga gattatgttc tgcacgccct
    17761 gggtatacgt aacaatggac aaatgtggta catttgcccg cgttgtcgcg gtatccccaa
    17821 cagaggatgt agagtcgtct tcggatgcat gggatgatga tgcagttttt cagggggcag
    17881 gatgggtaaa ctaacgctgc tgttgctggc tattctggtc tggctacagt attcgctgtg
    17941 gttcggtaag aacggtatac atgactatac ccgcgtcaat gatgatgtgg cggcactgca
    18001 agctacaaac gcgaaactta aagcgcgaaa cgatcaactt tttgccgaaa ttgacgatct
    18061 caatggcggc caggaggcgc tcgaagagcg tgcgcgtaat gaactcagca tgaccaggcc
    18121 gggcgaaact ttttatcgtc tggtgcctga cgcgtcgaag cgcgcacagt ctgcggggca
    18181 aaacaatcga taaatcagcc cgggaattaa catggcaacc actcatttgg atgtttgcgc
    18241 cgtggttccg gcggccggat ttggccgtcg aatgcaaacg gaatgtccta agcaatatct
    18301 ctcaatcggt aatcaaacca ttcttgaaca ctcggtgcat gcgctgctgg cgcatccccg
    18361 ggtgacacgt gtcgtcattg ccataagtcc tggcgatagc cgttttgcac aacttcctct
    18421 ggcgaatcat ccgcaaatca ccgttgtaga tggcggtgat gagcgtgccg attccgtgct
    18481 ggcaggtctg aaagccgctg gcgatgtgca gtgggtattg gtgcatgacg ccgctcgtcc
    18541 ttgtttgcat caggatgacc tcgcgcgatt gttggcgttg agcgaaacca gccgcacggg
    18601 agggatcctc gccgcaccag tgcgcgatac tatgaaacgt gccgaaccgg gcaaaaatgc
    18661 cattgctcat accgttgatc gcaacggctt atggcacgcg ctgacgccgc aatttttccc
    18721 tcgtgagctg ttacatgact gtctgacgcg cgctctaaat gaaggcgcta ccattaccga
    18781 cgaagcctcg gcgctggaat attgcggatt tcatccgcag ttggtcgaag gccgcgcgga
    18841 taacattaaa gtcacgcgcc cggaagattt ggcactggcc gagttttacc tcacccgaac
    18901 catccatcag gagaatacat aatgcgaatt ggacacggtt ttgacgtaca cgcctttggc
    18961 ggtgaaggcc caattatcat tggtggcgta cgcattcctt acgaaaaagg attgctggcg
    19021 cattctgatg gcgacgtggc gcttcatgcg ttgaccgatg cattgcttgg cgcggcggcg
    19081 ctgggggata tcggcaaact gttcccggat accgatccgg catttaaagg tgccgacagc
    19141 cgcgagctgc tacgcgaagc ctggcgtcgt attcaggcga agggttatac ccttggcaac
    19201 gtcgatgtca ctatcatcgc tcaggcaccg aagatgttgc cgcacattcc acaaatgcgc
    19261 gtgtttattg ccgaagatct cggctgccat atggatgatg ttaacgtgaa agccactact
    19321 acggaaaaac ttggatttac cggacgtggg gaagggattg cctgtgaagc ggtggcgcta
    19381 ctcattaagg caacaaaatg attgagtttg ataatctcac ttacctccac ggtaaaccgc
    19441 aaggcaccgg gctgctgaaa gccaatccgg aagactttgt ggtggtggaa gatttgggct
    19501 ttgagcctga tggtgaaggt gagcatattc tggttagaat cctcaaaaac ggctgcaata
    19561 cccgttttgt ggcggatgca ctggcgaaat tcctgaaaat tcattcccgt gaagtcagct
    19621 tcgctgggca aaaagacaaa catgctgtaa cggaacagtg gttatgtgct cgcgtgccgg
    19681 gcaaggaaat gcccgatctg agcgcctttc aactggaagg ctgccaggtg ctggagtatg
    19741 cgcggcacaa gcgcaagctg cgtttagggg cgctgaaagg taacgccttt accctggttc
    19801 tgcgcgaagt gagcaatcgc gatgacgttg aacaacgtct gatcgatatt tgcgtaaaag
    19861 gtgtaccgaa ctacttcggt gcccaacgtt ttgggattgg cggtagcaac ttgcagggcg
    19921 cgctgcgctg ggcgcaaacc aatactccgg tgcgcgatcg caataaacgg agtttttggt
    19981 tgtcggcagc ccgcagtgcg ttgtttaatc agattgttgc tgagcgcctc aaaaaagcag
    20041 acgttaatca agttgttgac ggcgatgcgc tacaattagc cggacgtggt agctggtttg
    20101 tagcaaccac cgaagaactg gcggaattac agcgtcgcgt caacgataaa gagctgatga
    20161 taaccgccgc attgccgggc agtggcgaat ggggaactca gcgtgaagcg ctggcattcg
    20221 aacaagcagc tgtcgccgca gaaactgaat tacaagcttt actggtgcgc gaaaaagttg
    20281 aagccgcgcg cagagcgatg ctgctgtatc cgcaacaatt aagctggaac tggtgggatg
    20341 acgtcaccgt agagatccgt ttctggcttc cggcgggtag ttttgcaacc agcgttgtca
    20401 gggaacttat caacacaaca ggtgattatg cgcatattgc tgagtaatga tgacggggta
    20461 catgcacccg gtatacaaac gctggcgaaa gccttgcgtg agtttgctga cgttcaggtg
    20521 gtcgcccccg atcgtaaccg cagcggcgct tcaaattctc tgacactgga atcctccctg
    20581 cgcacgttta cctttgaaaa tggtgatatt gctgtgcaaa tgggaacccc gaccgattgt
    20641 gtctatcttg gcgtgaatgc tctgatgcgt ccgcgcccgg acattgttgt gtccggaatt
    20701 aacgccgggc cgaatctggg ggatgatgtt atttattccg gtacggtagc cgccgcgatg
    20761 gaaggccgtc atttaggttt tccggcgctg gccgtctcgc ttgacgggca taaacattac
    20821 gacactgccg cggcggtaac ctgttcaatt ttgcgcgcac tgtgtaaaga gccgctgcgc
    20881 accggacgta ttcttaatat taacgttcag gatttaccct tggatcaaat caaaggtatt
    20941 cgcgtgacgc gctgcggtac acgacatccg gcagatcagg tgatcccgca gcaagatccg
    21001 cgcggcaata cgctgtactg gattggcccg ccgggcggta aatgtgatgc cggtccgggg
    21061 accgattttg ctgcggtaga tgagggctat gtctccatca cgccgctgca tgtggattta
    21121 actgcgcata gcgcgcaaga tgtggtttca gactggttaa acagcgtggg agttggcacg
    21181 caatggtaag cagacgcgta caagcacttc tggatcaatt acgtgcgcaa ggtattcagg
    21241 atgagcaggt gctgaatgca cttgccgccg tgccgcgtga aaaattcgtt gatgaagcgt
    21301 ttgaacaaaa agcctgggac aatatcgcct tgccgatagg tcaggggcag acaatttcgc
    21361 agccgtatat ggtggcgcga atgaccgaat tactcgagct gacgccgcag tcgcgggtgc
    21421 tggaaattgg cactggttcg ggatatcaaa cggcaatcct ggcgcatctt gtccagcatg
    21481 tttgctcggt tgaacggatt aaaggcttgc agtggcaggc acgtcgccgc ctgaaaaatc
    21541 ttgatttaca taatgtttca acccgtcatg gtgatggatg gcaaggttgg caggcacgtg
    21601 cgccgtttga cgctatcatt gttacggcgg caccgccgga aattccaact gcgctaatga
    21661 cgcagctgga cgaaggcggg attctcgtct tacccgtagg ggaggagcac cagtatttga
    21721 aacgggtgcg tcgtcgggga ggcgaattta ttatcgatac cgtggaggcc gtgcgctttg
    21781 tccctttagt gaagggtgag ctggcttaaa acgtgaggaa atacctggat ttttcctggt
    21841 tattttgccg taggtcagcg tatcgtgaac atcttttcca gtgttcagta gggtgccttg
    21901 cacggtaatt atgtcactgg ttattaacca atttttcctg ggggataaat gagcgcggga
    21961 agcccaaaat tcaccgttcg ccgcattgcg gctttgtcac tggtttcgct atggctggca
    22021 ggctgttctg acacttcaaa tccaccggca ccggtcagct ccgttaatgg caatgcgcct
    22081 gcaaatacta attctggtat gttgattacg ccgccgccga aaatggggac gatgtctaca
    22141 gcgcagcaac cgcaaattca gccggtacag cagccacaaa ttcaggctac tcaacaaccg
    22201 caaatccagc cagtgcagcc agtagctcag cagccggtac agatggaaaa cggacgcatc
    22261 gtctataacc gtcagtatgg gaacattccg aaaggcagtt atagcggcag tacctatacc
    22321 gtgaaaaaag gcgacacact tttctatatc gcctggatta ctggcaacga tttccgtgac
    22381 cttgctcagc gcaacaatat tcaggcacca tacgcgctga acgttggtca gaccttgcag
    22441 gtgggtaatg cttccggtac gccaatcact ggcggaaatg ccattaccca ggccgacgca
    22501 gcagagcaag gagttgtgat caagcctgca caaaattcca ccgttgctgt tgcgtcgcaa
    22561 ccgacaatta cgtattctga gtcttcgggt gaacagagtg ctaacaaaat gttgccgaac
    22621 aacaagccaa ctgcgaccac ggtcacagcg cctgtaacgg taccaacagc aagcacaacc
    22681 gagccgactg tcagcagtac atcaaccagt acgcctatct ccacctggcg ctggccgact
    22741 gagggcaaag tgatcgaaac ctttggcgct tctgaggggg gcaacaaggg gattgatatc
    22801 gcaggcagca aaggacaggc aattatcgcg accgcagatg gccgcgttgt ttatgctggt
    22861 aacgcgctgc gcggctacgg taatctgatt atcatcaaac ataatgatga ttacctgagt
    22921 gcctacgccc ataacgacac aatgctggtc cgggaacaac aagaagtgaa ggcggggcaa
    22981 aaaatagcaa ccatgggtag caccggaacc agttcaacac gcttgcattt tgaaattcgt
    23041 tacaagggga aatccgtaaa cccgctgcgt tatttgccgc agcgataaat cggcggaacc
    23101 aggcttttgc ttgaatgttc cgtcaaggga tcacgggtag gagccacctt atgagtcaga
    23161 atacgctgaa agttcatgat ttaaatgaag atgcggaatt tgatgagaac ggagttgagg
    23221 tttttgacga aaaggcctta gtagaagagg aacccagtga taacgatttg gccgaagagg
    23281 aactgttatc gcagggagcc acacagcgtg tgttggacgc gactcagctt taccttggtg
    23341 agattggtta ttcaccactg ttaacggccg aagaagaagt ttattttgcg cgtcgcgcac
    23401 tgcgtggaga tgtcgcctct cgccgccgga tgatcgagag taacttgcgt ctggtggtaa
    23461 aaattgcccg ccgttatggc aatcgtggtc tggcgttgct ggaccttatc gaagagggca
    23521 acttggggct gatccgcgcg gtagagaagt ttgacccgga acgtggtttc cgcttctcaa
    23581 catacgcaac ctggtggatt cgccagacga ttgaacgggc gattatgaac caaacccgta
    23641 ctattcgttt gccgattcac atcgtaaagg agctgaacgt ttacctgcga accgcacgtg
    23701 agttgtccca taagctggac catgaaccaa gtgcggaaga gatcgcagag caactggata
    23761 agccagttga tgacgtcagc cgtatgcttc gtcttaacga gcgcattacc tcggtagaca
    23821 ccccgctggg tggtgattcc gaaaaagcgt tgctggacat cctggccgat gaaaaagaga
    23881 acggtccgga agataccacg caagatgacg atatgaagca gagcatcgtc aaatggctgt
    23941 tcgagctgaa cgccaaacag cgtgaagtac tggcacgtcg attcggtttg ctggggtatg
    24001 aagcggcaac actggaagat gtaggtcgtg aaattggcct cacccgtgaa cgtgttcgcc
    24061 agattcaggt tgaaggcctg cgccgtttgc gcgaaatcct gcaaacgcag gggctgaata
    24121 tcgaagcgct gttccgcaag taagtaagca tctgtcagaa aggccagtct caagcgaggc
    24181 tggccttttt cttttgtttg ggaagcttgt gtttttgtcg cctggataag acacgtcagc
    24241 gtcctacccg gcaatcagca ttctatgtgc tcatcatctt gcgcaccaac tgcataaaca
    24301 gctcctgctc ttctccactc aaacgcccca aaaattcctc atccacgctg tcaccaatag
    24361 gtatcgccgc agcaagtagc ttttctcctt cggcagtcag ccagacaaag cgtcgccgct
    24421 tgtcagcgac gtcatgttca cgtctgacta agccacgatt ttccattctt gccaacattt
    24481 ctgctagcgt tgctttggtg ctgactgctg cttctatcag tgccacctgt tcaataccag
    24541 gtttatcagc aatggcacgc atcaccgcgt actgtggttt ggtcaggtca ggtaactcgt
    24601 gctgccagcg agccgtgtgc tgctgaaaaa gctgtcgtaa ctgatggaac gctttatttc
    24661 gtaacgccat gtaaactccg ggctaattct ctgtcgctat catagcggtt ttttagtgac
    24721 gaagaagatg ttgttacttt tcaatccttg ccgctgtgga aatgaaggag tatgttaata
    24781 ataacgttcg tatacgaata attaagagaa taagcaatga aactgatcgt cgggatgaca
    24841 ggggctaccg gtgcgcctct tggtgtggca ttactgcaag cgctgcgtga gatgccgaat
    24901 gtcgagactc atctggtgat gtcgaagtgg gcgaaaacca ccattgaact ggaaacgcct
    24961 tacagcgctc gcgatgttgc tgccctcgca gacttcagcc ataacccggc ggatcaggcg
    25021 gcgatcatct catccggttc ttttcgtacc gacggcatga tcgttattcc gtgcagtatg
    25081 aaaacgctcg ccggtatccg cgctggttac gccgatggcc tggtagggcg cgcggcggac
    25141 gtcgtgctca aagaaggccg caaactggtg ctggtgccgc gtgaaatgcc gcttagcacc
    25201 atccatctcg aaaatatgct cgcactttca cgcatgggcg tggcgatggt gccgccgatg
    25261 cctgcctttt ataaccatcc cgaaacggta gatgacattg tccaccatgt ggtagcccgc
    25321 gtgctggatc aatttggcct cgaacatccc cacgccaggc gctggcaagg attaccgcag
    25381 gcccggaatt tttctcagga gaatgaataa tggcatttga tgatttacgc agctttttac
    25441 aggcgcttga tgaccacggc cagttactga aaatcagcga agaagtgaac gctgagccgg
    25501 atctggcagc agcagctaac gccaccgggc gtatcggcga cggcgcgccc gcgctgtggt
    25561 ttgataatat tcgcggcttt accgatgccc gcgtggcgat gaataccatc ggttcctggc
    25621 agaaccacgc gatttccctt ggcctgccac caaacacccc ggtcaaaaag cagattgatg
    25681 agtttatccg ccgctgggat aacttcccga ttgccccgga gcgccgcgcc aatccagcct
    25741 gggcgcagaa caccgtcgat ggcgaagaga tcaacctgtt cgatattctg ccgctgtttc
    25801 gcctgaatga tggcgatggc ggtttctatc tcgacaaagc gtgcgtggtt tcccgcgatc
    25861 cgctcgaccc ggataacttc ggcaagcaga acgtcggcat ctaccgcatg gaagtgaagg
    25921 gcaagcgtaa gctcggcctg caaccggtgc cgatgcacga tatcgccctg catctgcata
    25981 aagcagaaga gcgcggtgaa gatctgccga ttgcgatcac gctcggtaac gatccgatca
    26041 tcacgctgat gggggccacg ccgctgaaat acgatcagtc tgagtatgaa atggcaggcg
    26101 cgctgcgtga aagcccgtac ccgatcgcca ccgctccgct gaccggtttt gatgtgccgt
    26161 ggggttcaga agtgatcctc gaaggggtta tcgaaagccg taaacgcgaa atcgaagggc
    26221 cgttcggtga gtttaccggg cactactccg gtgggcgcaa catgaccgtg gtgcgcatcg
    26281 ataaagtctc ttatcgcagc aaaccgattt tcgaatcact ctatctcggt atgccgtgga
    26341 ccgaaatcga ctacctatga ctaggtaatt tattcaagat aaccgggaag agattaaaaa
    26401 agacgggaat agattggaat caacaagtta ggaactcaag gttttcaaga taaagcgagt
    26461 ccaaaaatgt taaacggttc gatatcaata aaacattcaa gataactcac aggttcttac
    26521 aatagccttt aaagaccgtt caaaaacgat tcaggtgttt attatagcgc tgatgggtgc
    26581 aagcgccaaa accaatgtaa ttttagcaca gctaggcact tgcactgggt tatgcgctgg
    26641 ctgctttctg aggtagattt aagtgatcgc ccttagcttc ggacaacgcg taaatctctc
    26701 taaccttttc gtctggcaaa ttaccaaggc ttaacgctat ggctctacga actccaacta
    26761 gagcgtgcaa ctcttggatg tgctcatcca gaagaatgag taattgctcc gctccttttc
    26821 tcccaagaac atcagctact tgttgttttt cttgtggttt aagaaatgac aagaacatta
    26881 gaatttgagc atcaacatta tccgcattct cttttttggg ttctttggaa tcttttagtt
    26941 caacatcacc ctctcccgtc atgagccatt ccaaggtaac attttcgctt tctgctatct
    27001 tttttaactg atgtgggcgt ggtatcggtg cgtaatcccg cctgttgtaa taatttttta
    27061 aagtgccttc attgatcccc catgcccgag cagccgcatt tcttgaaggg tatcgttcta
    27121 ctaacctaaa gattctttcg ataatcggtt cttttccatc ggcaggaaaa gaaccttggg
    27181 gtgcttttct cattcttggt tcctttctat ctaattcatt gataaataat aaaattaaaa
    27241 aataaaccat aaaaacacca aagaaccctt tacagggaat ctttggtatc ttaatcttta
    27301 tccatacgga tatccgaccg gatatccgcg cgggtaattt tgtaggttaa aggaaagatg
    27361 agacaaagag aagatagtga ccgcgattgg ccttcgctga agattcagct ggcgctcaaa
    27421 gataacgggt ggtcttcctt ccgggagctt tcactccatg ctggttatgc tcaggattca
    27481 ctgaaagcgg tagttcgcac accaaacaaa aaatacgaac gcgtaatttc tgacgctata
    27541 ggtgtaccgc cagaaatcat ctggcccagc cggtatcgta acgatggatt tatcaggaag
    27601 gcgtcgtgat atgcagtcgt acgttaccgt aaatgacctg ctcggcgtac caggaatgcc
    27661 agccaccaca aaaggcatcc gacaggcgtt acagcgtttt tctcgtgatc ttggtgacgt
    27721 tagtcgccgt cgggaaggca caaaagccat tgagtaccac attgattgtc tgcctgaaat
    27781 aacacgaaag gcactgcgtg aacgctatgt ggaacagctg gtggctacag agaacaatgt
    27841 ttctgaagtg aaagctgtta ccagaaaaac acgcaatcct gacgctgtcc aggcaatcga
    27901 agcatatcgc ggttctccac aactgatgga agaacgcctg aatgcgctga ccgaaaacca
    27961 gcgctgggta tctgaagcaa gagctgcgct ggtggtggaa gtgctgaagc tggaaagcgc
    28021 cggtaacccc gggcgactga aagccattaa ctttcttgtt gaaaaagccc gtaaaggtga
    28081 gctgccggag cgcctgcaac aggccgcagt taacgccaat gcaaaacgtg gcgctaatcg
    28141 tactatcagc cgcgatccac tttaccagtg ggttctgaaa tacaaccagt cgcagaacgc
    28201 cgccgaacgg ctgctgttac tggcaccggg aaaacgcgac gaaataaaac cggaagaaat
    28261 cagctggctg cctgaatttc tggcgcagta tcgccaggtg aacggcagac ccatgtcaga
    28321 agcctacgag gattttgtcg cagaatggca gcgtcgccat gcggacgaac cgtacatgct
    28381 ggaggtgatg ccttcctatg atgttgtccg gtatgccatg aaaaaactgc cggaagtggt
    28441 gaaacaaaaa gggcgcgtta ccggcagtga ataccgtcag cttgaaggat tcacccgccg
    28501 cgactggaca gctatgccgg tgaattatgt ctggattggt gacggtcacg gtatgaagct
    28561 gaaatgtgcg catccgatcc acgggcgtcc attctcaccg gaagtcacgt ttgtgatcga
    28621 tggtggaaca cggtttgttg ttggctggag tcttgacctt gccgaaaacg tgttcgccgt
    28681 tgccggtgcg atacagcacg gcattcgcaa ccacggaaaa ccgttcctgt attactcgga
    28741 taacggttcc ggtgaaacgg ctgacatgct ggacaaagag gtcgtgggta ttctgccacg
    28801 cctggggatt aaacacccta ccggtattgc cggtaatcca cagggacgcg gcattattga
    28861 acgcctgaac cggacactgc cgatgcgtat agccaggagg tatcgcacct attttggcaa
    28921 gggtgctgac cgggaatcgt tgcgtgtact taaccgtgat ctgcgttcag cgtttaacgc
    28981 cctgcaacag gacaagcctc tgaacgaccg ccagaaagcg gcaatgcgcg agctgccatc
    29041 gtgggcggag cttattgagg ctattcgtga aggcgtggaa tggtacaaca accgcccgca
    29101 ttctgagctg ccgatgaaac cgaacggacg gcattacagc ccgacagagt tcagaaaaaa
    29161 acgccaggca gaagaagaca ccgaaattga gtggctgagt gatcttgagc tgcgcgacat
    29221 gttccgccca atggtggaac gaccggtaag acgctgcgaa attcagtggc tgaacaatat
    29281 ttactacgcg ccggagttgc gtgacgaaca cggtcgcaaa gtgcttatca gttatgacat
    29341 tcatgacgcc gagcgaatta cggtacgtcg caaggacggc agctttatct gtgaggcgat
    29401 atggaacggc aataaacgcg cagcctttgc tgtcagtgct gaatatcaca aacaacagca
    29461 gcgtattaaa ggaatgcgca aacgcgctga ggaaaaaatt cgtgatgctg aagatgaagg
    29521 tattcagatt ctggaacaca aacaggctga accctggctg agtaatgttt atcgcccggt
    29581 tggtaatgtg gtggcagttc agcaaccgga atatgaagaa gagcatgacg aagaatttga
    29641 acgcgatttc cggctgggga tgcaaaaact ttttgccatg caggaagaag atgatccgct
    29701 ggcctgaata aaaaaacagt ccgcgggaac ggactgttaa ataaaggaaa caaagctgat
    29761 ttgaaataac cgaggaaata ctatgactga tattgatgat gt
//
LOCUS       DAEEUE010000043        29236 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.043, whole genome shotgun sequence.
ACCESSION   DAEEUE010000043 DAEEUE010000000
VERSION     DAEEUE010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29236
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.043"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..218
                     /locus_tag="KLK84_002079"
     CDS             <1..218
                     /locus_tag="KLK84_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE2009399.1"
                     /translation="DTAMNFIRQGLGIALQPELTLKSIAGELCSVPHEPTFYRQISLL
                     TKEKPVEGSPLFLLQMCMEQLVAIGKI"
     gene            complement(260..1336)
                     /gene="glpQ"
                     /locus_tag="KLK84_002080"
     CDS             complement(260..1336)
                     /gene="glpQ"
                     /locus_tag="KLK84_002080"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBE2009400.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(1341..2699)
                     /gene="glpT"
                     /locus_tag="KLK84_002081"
     CDS             complement(1341..2699)
                     /gene="glpT"
                     /locus_tag="KLK84_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HBE2009401.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            2972..4600
                     /gene="glpA"
                     /locus_tag="KLK84_002082"
     CDS             2972..4600
                     /gene="glpA"
                     /locus_tag="KLK84_002082"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HBE2009402.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            4590..5849
                     /gene="glpB"
                     /locus_tag="KLK84_002083"
     CDS             4590..5849
                     /gene="glpB"
                     /locus_tag="KLK84_002083"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HBE2009403.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            5846..7036
                     /gene="glpC"
                     /locus_tag="KLK84_002084"
     CDS             5846..7036
                     /gene="glpC"
                     /locus_tag="KLK84_002084"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HBE2009404.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            7229..8179
                     /locus_tag="KLK84_002085"
     CDS             7229..8179
                     /locus_tag="KLK84_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HBE2009405.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8220..8690)
                     /locus_tag="KLK84_002086"
     CDS             complement(8220..8690)
                     /locus_tag="KLK84_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBE2009406.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(8745..9947)
                     /locus_tag="KLK84_002087"
     CDS             complement(8745..9947)
                     /locus_tag="KLK84_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HBE2009407.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(10034..10576)
                     /locus_tag="KLK84_002088"
     CDS             complement(10034..10576)
                     /locus_tag="KLK84_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009408.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            10855..11280
                     /gene="nudI"
                     /locus_tag="KLK84_002089"
     CDS             10855..11280
                     /gene="nudI"
                     /locus_tag="KLK84_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HBE2009409.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(11319..11921)
                     /locus_tag="KLK84_002090"
     CDS             complement(11319..11921)
                     /locus_tag="KLK84_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBE2009410.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            12229..13368
                     /gene="arnB"
                     /locus_tag="KLK84_002091"
     CDS             12229..13368
                     /gene="arnB"
                     /locus_tag="KLK84_002091"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HBE2009411.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAESGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRTAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            13372..14340
                     /gene="arnC"
                     /locus_tag="KLK84_002092"
     CDS             13372..14340
                     /gene="arnC"
                     /locus_tag="KLK84_002092"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HBE2009412.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            14340..16322
                     /gene="arnA"
                     /locus_tag="KLK84_002093"
     CDS             14340..16322
                     /gene="arnA"
                     /locus_tag="KLK84_002093"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HBE2009413.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEIFQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFLPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSCYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            16319..17209
                     /locus_tag="KLK84_002094"
     CDS             16319..17209
                     /locus_tag="KLK84_002094"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HBE2009414.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            17209..18861
                     /gene="arnT"
                     /locus_tag="KLK84_002095"
     CDS             17209..18861
                     /gene="arnT"
                     /locus_tag="KLK84_002095"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HBE2009415.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLVLAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            18858..19193
                     /gene="arnE"
                     /locus_tag="KLK84_002096"
     CDS             18858..19193
                     /gene="arnE"
                     /locus_tag="KLK84_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HBE2009416.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVSQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            19193..19579
                     /gene="arnF"
                     /locus_tag="KLK84_002097"
     CDS             19193..19579
                     /gene="arnF"
                     /locus_tag="KLK84_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HBE2009417.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(19573..19845)
                     /gene="pmrD"
                     /locus_tag="KLK84_002098"
     CDS             complement(19573..19845)
                     /gene="pmrD"
                     /locus_tag="KLK84_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HBE2009418.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(19950..21305)
                     /gene="menE"
                     /locus_tag="KLK84_002099"
     CDS             complement(19950..21305)
                     /gene="menE"
                     /locus_tag="KLK84_002099"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HBE2009419.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(21302..22264)
                     /gene="menC"
                     /locus_tag="KLK84_002100"
     CDS             complement(21302..22264)
                     /gene="menC"
                     /locus_tag="KLK84_002100"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HBE2009420.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(22264..23121)
                     /gene="menB"
                     /locus_tag="KLK84_002101"
     CDS             complement(22264..23121)
                     /gene="menB"
                     /locus_tag="KLK84_002101"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HBE2009421.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(23136..23894)
                     /gene="menH"
                     /locus_tag="KLK84_002102"
     CDS             complement(23136..23894)
                     /gene="menH"
                     /locus_tag="KLK84_002102"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HBE2009422.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(23891..25561)
                     /gene="menD"
                     /locus_tag="KLK84_002103"
     CDS             complement(23891..25561)
                     /gene="menD"
                     /locus_tag="KLK84_002103"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HBE2009423.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(25650..26945)
                     /gene="menF"
                     /locus_tag="KLK84_002104"
     CDS             complement(25650..26945)
                     /gene="menF"
                     /locus_tag="KLK84_002104"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HBE2009424.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(27024..27329)
                     /gene="elaB"
                     /locus_tag="KLK84_002105"
     CDS             complement(27024..27329)
                     /gene="elaB"
                     /locus_tag="KLK84_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HBE2009425.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(27384..27845)
                     /locus_tag="KLK84_002106"
     CDS             complement(27384..27845)
                     /locus_tag="KLK84_002106"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE2009426.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            27910..28827
                     /gene="rbn"
                     /locus_tag="KLK84_002107"
     CDS             27910..28827
                     /gene="rbn"
                     /locus_tag="KLK84_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HBE2009427.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            29018..29206
                     /locus_tag="KLK84_002108"
                     /pseudo
     CDS             29018..29206
                     /locus_tag="KLK84_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         7086 a         7856 c         7300 g         6994 t
ORIGIN      
        1 cggatacagc gatgaatttt attcgacagg gattaggcat cgccttacaa ccagagttaa
       61 cgctgaaaag cattgcaggt gaattgtgtt ccgttcctca cgaaccaacc ttctatcgac
      121 agatttcgtt gctgactaaa gaaaagccgg tagaaggcag tccactgttt ttactacaaa
      181 tgtgcatgga acaattagtg gcgattggaa aaatttgata tgagacagga tggcgcatga
      241 acgccatcct gcagtaacat tactctttat taagaaattt tactgcctta tcagggaaat
      301 cagtaaacag cccatttaca cccgctttgt tatacagagc atcatataac tgattcacat
      361 caggagtgta ttcaggcagt ttatctgacc gcacggtata aggatgcact accagtttat
      421 tctgctgggc atcttgcacc atgccagtga gtttgatatt acccggctgc gatgtctcct
      481 caatcaacat atggtaatcc ggaccaatac catctgcata ttccgccacc tgtttcatgg
      541 ctcccggctt aaacatccag tcgtagttgt aattaaccca gcttccatcc ggctgtttct
      601 gctgcgtttc attccagtcg gtataggcaa tcagctgtac cagattgagc tccatgccca
      661 ttttgggttc cagctcattc ttaatacgct tcagctcatc agcatcaaaa cattgcaaat
      721 aaactttatc gtctttaccg gtgtaaccat atttcttcag cacttccagc gtttttgccg
      781 caatatcctt cccttcctga tgatggaacc acggcgcttt gatttctgga tagataccga
      841 tatttttccc ggtagagtga tttaacccct gaacaaattc aatctcttct tcaaaggtgt
      901 gcacccggaa gtcggactta cccattggga aacgccccgg ataagtctgt acttttttac
      961 cgttttcaat atcgaaacct tcggtaaatt tcagcgactt aatttcatcc agcgtgaaat
     1021 ctatcgcgta gtaacgaccg tctttgcgcg cccgatccgg gaaacgatcg gcaacatcag
     1081 taacacgatc gagataatgg tcatgcagaa caaccagatg gtcgtctttg gtcatcacca
     1141 aatcctgttc cagataatcc gctccctgcg catacgccat cgcttttgct ggcagcgtat
     1201 gctccggcaa atatccactg gcaccgcgat gggcgatgac tattttttcg ttgctgtccg
     1261 ccgccattgc actgcttccc atgactatag tgctcatcat gatcgccatg ctaaggtttt
     1321 tcagcttcaa tttcatgcca ttagcctccg ttgcgttttt gcagtaattg ttcatggcga
     1381 cgtttttcgc caatcatcac aacaatcaac aagataaccg ccagaatgct gccgccaatc
     1441 attaccataa agccgccatc ccagccgaag aagtccacgg tgtagccaac aatcgcgctc
     1501 gccgccaccg aaccgcccag gtaaccaaac agcccggtaa agcccgctgc cgtacctgcc
     1561 gcttttttcg gtgccagttc cagcgcatgc agaccgatca gcatcacagg accgtagatc
     1621 aggaagccga taacaatcat acaaatcata tcgacggttg ggttacctgc cgggttcatc
     1681 cagtaaacga tagtcgcgat ggtcaccagt gtcataaaga agacgccggt tgccccacgg
     1741 ttaccacgga agactttgtc cgacatccag ccgcacagca gggtgcccgg aatacccgca
     1801 tactcataga agaagtaggc ccaggaggat ttatccagcg cgaaatgctt aacctctttc
     1861 agataagtcg gtgaccagtc gaggatgccg taacgcagca gataaacgaa cacgttggcg
     1921 atggcgatgt accacagcag tttgttcggc agcacgtact gcatgaagat ttgcttcgcg
     1981 gtcagctcct gttccgcttt ttcgttatag tcgtccggat aatcattttt gtactcttcg
     2041 atcggcggca agccacagga ttgcggggta tcgcgcatca tcgcaaaggc gaataatgcc
     2101 accagaatgg cgcagaaagc aggcatatag agcgccgcat gccagtcatt gaaccaggcc
     2161 atccccagca ggaacagcag cggcggaata ccaccaccga cgttgtgcgc acagttccac
     2221 actgacacaa tgccgccacg ttctttctgc gaccaccagt gcaccatagt acgaccacac
     2281 ggcggccacc ccatcccctg gaaccaaccg cagaggaaca acagtacaaa catcaccgca
     2341 atgctcgacg tcgcccatgg cacaaagccc ataaacaaca tcactgccgc cgccagaatc
     2401 aaacctgcgg gcaggaaaac gcgcggattc gagcgatccg ataccgaacc catgatgaat
     2461 ttcgaaaatc cataagcaat cgagatcccc gaaagggcaa aacctaaatc accgcgtgag
     2521 aatccctgct caaccagata aggcatagca agcgcaaagt tcttacgaac caaatagtaa
     2581 gccgcatagc caaagaatat ccccaggaaa atttgccagc gcaatcgacg ataagtcgga
     2641 tcgatctccg cggcaggtaa gcgcgctttg tgtggcgctg gtttaaaaat actcaacatt
     2701 gatagcctcc gtggcccgtg gtcttattta tgattaacag cctgattcag tgagagaacc
     2761 tgccgtttct tgagttgccg cgatgttaag aaaacattca taaattaaat gtgaattgcc
     2821 gcacacatta ttaaataaga tttacaaaat gttcaaaatg acgcatgaaa tcacgtttta
     2881 ctttcgaatc atgagcgaat atgcgcgaaa tcaaacaatt catgttttta ctatggctaa
     2941 atggtaaaaa acgaacttca gagggataac aatgaaaact cgcgactcgc aatcaagtga
     3001 cgtgattatc attggcggcg gcgcaacggg agccgggatt gcccgcgact gtgccctgcg
     3061 cgggctgcgc gtgattttgg ttgagcgcca cgacatcgca accggtgcca ccgggcgtaa
     3121 ccacggcctg ctgcacagcg gtgcgcgcta tgcagtaacc gatgcggaat cggcccgcga
     3181 atgcattagt gaaaaccaga tcctgaaacg cattgcacgt cactgcgttg aaccaaccaa
     3241 cggcctgttt atcaccctgc cggaagatga cctctcattc caggccactt ttattcgcgc
     3301 ctgcgaagaa gcagggatca gcgcagaagc tatagacccg cagcaagcgc gcattatcga
     3361 acctgccgtt aacccggcac tgattggcgc ggtgaaagtt ccggatggca ccgttgatcc
     3421 atttcgtctg accgcagcaa acatgctgga tgccaaagaa cacggtgccg ttatccttac
     3481 cgctcatgaa gtcacggggc tgattcgtga aggcgcgacg gtgtgcggtg ttcgtgtacg
     3541 taaccatctc accggcgaaa ctcaggccct tcatgcacct gtcgtggtta atgccgctgg
     3601 gatctggggg caacacattg ccgaatatgc cgatctgcgc attcgcatgt tcccggcgaa
     3661 aggatcgctg ctgatcatgg atcaccgcat taaccagcat gtgatcaacc gctgccgtaa
     3721 accttccgac gccgatattc tggtgcctgg cgataccatt tcgctgattg gtaccacctc
     3781 tttacgtatt gattacaacg agattgacga taatcgagtg acggcagaag aggttgatat
     3841 tctgctgcgt gaaggggaaa aactggcccc cgtgatggcg aaaacgcgca ttttgcgggc
     3901 ctattctggc gtgcgcccgc tggttgccag cgatgacgac ccgagcggac gtaacgtcag
     3961 ccgtggcatc gtgctgctcg accatgctga acgcgatggt ctggacggat ttatcaccat
     4021 caccggtggc aaactgatga cctatcggct gatggctgaa tgggctaccg acgcggtatg
     4081 ccgcaaactg ggcaacacgc gcccctgtac gactgccgat ctggcactgc ctggttcaca
     4141 agaacccgct gaagttacct tgcgtaaagt catctccctg cctgccccgc tgcgcggttc
     4201 tgcggtttat cgtcatggcg atcgcacgcc tgcctggctg agcgaaggcc gtctacaccg
     4261 tagcctggta tgtgagtgcg aagcggtaac tgcgggtgaa gtgcagtacg cggtagaaaa
     4321 tttaaacgtt aatagcctgc tggatttacg ccgtcgtacc cgtgtgggga tgggcacctg
     4381 ccagggcgaa ctctgcgctt gccgcgctgc cggactgctg caacggttta acgtcacgac
     4441 gtccgcgcaa tctatcgagc aactttccac cttccttaac gaacgctgga aaggcgtgca
     4501 acccatcgcc tggggagacg cactgcgcga aagcgaattt acccgctggg tttatcaggg
     4561 actgtgtggt ctggagaagg agcagaaaga tgcgctttga tactgtcatt atgggcggtg
     4621 gcctcgccgg attactctgt ggcctgcaac tgcaaaaaca cggcctgcgc tgtgccattg
     4681 tcacgcgtgg tcaaagcgcg ttgcatttct catccggttc gctggatttg ctgagccatc
     4741 tgccagatgg tcaacccgtg gcagacattc acagtggact ggaatctttg cgtcagcagg
     4801 cacctgcaca tccttactct cttctcgggc cacaacgcgt gctcgatctc gcttgccagg
     4861 cgcaggcatt aatcgctgaa agcggtgcgc aattgcaggg cagcgtagaa ctggctcacc
     4921 agcggattac gccgctcggc actttgcgtg caacctggtt aagttcgcca gaagttcccg
     4981 tctggccgct acccgcgaag aagatatgtg tagtggggat aagcgggctg atggatttcc
     5041 aggcgcatct tgcggcggct tcgttgcgcg aactggatct ttcggttgaa accgcagaaa
     5101 tagagctgcc ggaactggat gtcctgcgca ataacgccac cgaatttcgc gcggtgaata
     5161 tcgcccgttt ccttgataat gaagaaaact ggccgctgtt acttgatgcg cttattcctg
     5221 tcgccaatac ctgcgaaatg atcctgatgc ccgcctgctt cggcctggcc gatgacaaac
     5281 tgtggcgttg gttgaatgaa aaactacctt gttcactgat gcttttgcca acgctgccgc
     5341 cttccgtgct gggcattcgt ctgcaaaacc agttacagcg ccagtttgtg cgccaaggtg
     5401 gagtgtggat gccgggcgat gaagtgaaaa aagtgacctg taaaaatggc gtagtgaatg
     5461 aaatctggac ccgcaatcac gccgatattc cgctacgtcc acgtttcgcg gttctcgcca
     5521 gcggcagttt ctttagtggc ggactggtag cggaacgtaa cggcattcgc gagccgattc
     5581 tcggccttga tgtgctacaa accgccacgc ggggtgaatg gtataagggc gatttttttg
     5641 cgccgcaacc gtggcagcag ttcggtgtaa ccactgatga gacgctacgc ccgtcgcagg
     5701 cagggcaaac cattgagaac ctgtttgcca tcggttcggt gctgggcgga tttgatccca
     5761 tcgcccaggg atgcggcggc ggtgtttgtg ccgtcagtgc tttacatgcc gctcaacaga
     5821 ttgcccaacg cgcaggaggc caacaatgaa cgacaccagc ttcgaaaact gcattaagtg
     5881 caccgtctgc accaccgcct gcccggtgag ccgggtgaat cccggttatc cagggccaaa
     5941 acaagccggg ccggatggcg agcgtctgcg tttgaaagat ggcgcactgt atgacgaggc
     6001 gctgaaatat tgcatcaact gcaaacgttg tgaagtcgcc tgcccgtccg atgtgaagat
     6061 tggcgatatt atccagcgcg cgcgggcgaa atatgacacc acgcgcccgt cgctgcgtaa
     6121 ttttgtgttg agtcataccg acctgatggg tagcgtttcc acgccgttcg caccaatcgt
     6181 caacaccgct acctcgctga aaccggtgcg gcagctgctt gatgcggcgt taaaaatcga
     6241 tcatcgtcgc acgctaccga aatactcctt cggcacgttc cgtcgctggt atcgcagcat
     6301 agcggctcag caagcacaat ataaagacca ggtcgctttc tttcacggct gcttcgttaa
     6361 ctacaaccat ccgcagttag gtaaagattt aattaaagtg ctcaacgcaa tgggtaccgg
     6421 tgtacaactg ctcagcaaag aaaaatgctg cggcgtaccg ctaatcgcca acggctttac
     6481 cgataaagca cgcaaacagg caattacgaa tgtagagtcg atccgcgaag ctgtgggagt
     6541 aaaaggcatt ccggtgattg ccacctcctc aacctgtaca tttgccctgc gcgacgaata
     6601 cccggaagtg ctgaatgtcg acaacaaagg cttgcgcgat catatcgaac tggcaacccg
     6661 ctggctgtgg cgcaagctgg acgaaggcaa aacgttaccg ctgaaaccgc tgccgctgaa
     6721 agtggtttat cacactccgt gccatatgga aaaaatgggc tggacgctct acaccctgga
     6781 gctgttgcgt aacatcccgg ggcttgagtt aacggtgctg gattcccagt gctgcggtat
     6841 tgcgggtact tacggtttca aaaaagagaa ctaccccacc tcacaagcca tcggcgcacc
     6901 actgttccgc cagatagaag aaagcggcgc agatctggtg gtcaccgact gcgaaacctg
     6961 taaatggcag attgagatgt ccacaagtct tcgctgcgaa catccgatta cgctactggc
     7021 ccaggcgctg gcttaaactc ccttctgatg cccggtaagc atgtggttac cgggcatttt
     7081 tgcgtacacg attccgtgcc caatgtatgc gttgcaacgc agtgaaaatt cctctgaaaa
     7141 cgtctcgcaa aggctgaaac tggcagatgt caaaggcctg ggataaccgt aatgtcgcgt
     7201 catcataaat atcaggtgac ggacaaccat gaccgaatca acaacctcct ccccgcatga
     7261 tgcggtattt aaaaccttta tattcacacc cgaaaccgca cgggattttc tcgaaataca
     7321 tttaccagaa ccactgcgca agctttgcaa cctgcaaacc ttacgcctgg aacccactag
     7381 ttttattgaa aaaagtttac gcgcttacta ctcggatgtt ttgtggtccg tggaaaccag
     7441 cgacggtgac ggctatatct actgcgtgat tgaacatcaa agctctgcag aaaagaatat
     7501 ggcttttcgg ccaatgcgct atgccactgc cgccatgcag agtcacctag ataaaggcta
     7561 tgacagagtt ccgctggtgg tgccattgct gttttatcat ggcgaaacat cgccctaccc
     7621 gtactcactt aactggctgg atgagtttga cgatccgcaa cttgcccggc agttgtacac
     7681 cgaagctttt ccgttggtgg atatcaccat cgtacctgac gatgagatca tgcaacatcg
     7741 gcgtatagct ctgctggaac tgattcaaaa gcatattcgc gaccacgatt taatcggcat
     7801 ggtcgacagg atcaccacgc ttttggttag aggcttcact aatgacagcc agctacaaac
     7861 actgtttaat tatctgctgc aatgcggcga tacctcccgt ttcacccgtt ttattcagga
     7921 gattgccgaa cgttcaccac tacaaaagga gagattaatg actattgctg aacggctacg
     7981 gcaggaagga catcaaattg gctggcaaga aggtaaatta gtaggtttgc agcaaggtaa
     8041 attagaaggt ttgcaggaag gtatgcatga acaagccatt aaaattgctt tgcgcatgct
     8101 ggaacagggc attgatcgtg accaagtgct cgcggccacc cagctaagcg aagccgatct
     8161 ggcagcgaat aaccactaat taacacaggc ccacagccga tccccatggg cctttgatat
     8221 caataactac cttttatgcg tggcccattt ttgcctgatt taaacatcgc cagtcgttga
     8281 tccagggcat cgctgtagag catcgtgtca acgccaacag cgacaaagtt cgctccccac
     8341 gccaggcatt gctgcgccat atcaggagcc acagccagaa aaccagccgc tttacccgca
     8401 gcacggatcc gccgaatgct ggtttcaata attcgctgca cttccgggtg cccggcgtta
     8461 tccgggtagc ccaacgacgc agaaagatcc gctggtccaa taaacacgcc atcaatccct
     8521 tcgacgtcga ggatctcgtc caggttatcc agtgccgttt tactttccac ctgcactaac
     8581 agacaaagcg aatcgttgac ttgcgccatg taattctcaa tgcgtcccca gcgcgcagcc
     8641 cgtgccacac tggccccaac accacgctca ccgtagggag ggttggacaa attttgccgg
     8701 agggggaatg tgccggtgca gtaaaccggc acagagtggc gaaatcagac ggataaagtc
     8761 atggactcaa caacctcaat ccagccatgc tcactagcga tgtcctggcc gtttaaccaa
     8821 cggcgtagca tattcagtgc catcattgcg cacacttcct gacggatagc caggctgtag
     8881 cgtgtagtgc tgaaacgcac acgcagagcg aaagtgccgt ctggcgtggc tagcgcaaag
     8941 ttgagatgct cgttctcgaa acccgaaaca gccagtgcca gcccggcaaa atggttggcc
     9001 cgccgttctg taatccagtg cgcagtttgc gccagggttt cttcctgtga aggaatcact
     9061 tcacacgcca gcaatggagc acctgcgcga gaaagttgca aagccaataa accaccggtg
     9121 aattgctcgc tcaacgtcag gctgaactgg cggttttgca attcgcgact gatctgcgcg
     9181 ggcagtcctt cggtgccttc gaaaatcacg ctctgtccgg caacacgttt aacgtccagc
     9241 cacagttttt ccatcgcctg ttgctcgctt gccggtccgg tgagtttcag ttcgatgatc
     9301 ggcattgagg agcgatagcc cattgttacg cccggcggca gttgtagagt gtccaggctt
     9361 tgtgccagat cgctttccga acgaccaaag gtagttaaac gcagacaaac cggcggctgt
     9421 ggtaaagaaa aacgctcgcg caggcgcggc aggatttcgt gctcgaccat caccttaaat
     9481 tctgacggta cgccgggagt aaagaacatc aggcaacgat taagctgcac ggcaaaacca
     9541 caggcggtgc ctaccgggtt attgataaat tcagcactgg caggcagctc cgcttgttta
     9601 cggttgctcg gtgccattac tcgtccacgt tcgtggaaat agcgttccat ctctttgagc
     9661 caggcttcat gcagcaccag gccttcacct tttgctgtcg cagcggcgag tgcgcttaaa
     9721 tcatcgctgg tcggtcccag cccgccgtta acgatcagca catcggcgtg ctgactacgt
     9781 tcgcgaagaa tggtgactaa gtcatcaagg ttatccccca ccgtattgcg gcgagataat
     9841 ggcagcccct gatgaaagaa aaaatcggcc agccaggcag cgttagtgtc aacgatttgc
     9901 ccgtataaca cttcatcccc ggtggatagc atttccactt ttaacatggt tatctcctgc
     9961 ttttgttatc gaatcactat aacgcaaagg gagataaggc gtagtaagaa actgaatgcc
    10021 gcgccggaag ggatcagaaa ctggcgttaa ccccaacata caggccgtca gccacagcgt
    10081 tgtcgcggtt accgtcttta cccgacagat tcaggtagcg ataacccgcc tcaatactga
    10141 ctggacgcat aatggtgtaa cgcgcgccag cattcgcttc ctcataactt tgaataccgc
    10201 tggagagcga atccggagag tagtaatact cgccaaacaa acggaagctg ttgccaattt
    10261 tccactgcaa accacctcct accgctgcgg cataaccttc atcgccgtaa ttcgggttgg
    10321 tgtacacgcc ttttccgcca acggtcgcca ttaacggccc gagaggcaga ttcaacccca
    10381 gccccacgcc cgcgacgtca ccgtcgtcgt cgttatgtgt ccagttaccg cttaaagcca
    10441 ggcccgtcga ttcagtccca aaaccgacac caatattggt gtattcttta cccgcctggc
    10501 cgctgatgct cattgcattg accgatgcag aaaccaacag cattccggcc agacctgcta
    10561 gcgcaatttt tttcattatc ccctttccca caagcaatta atcaaaattc ccaaagctgc
    10621 gggattgtac tgcctggcac agaggattca atgagcgaaa aaagggatct agtcacttat
    10681 tgtaactaaa tacttcttaa ataattaaac gccgaaaaaa taaacacact ttaccgatac
    10741 acacaaaaat aataataagt gcagttatca ttgcaaaaga gattactgtc actttcacat
    10801 atttctgagt taccgtattc tcatggtttc tttttattta taaggaatac ccctgtgcga
    10861 caacggacta ttgtatgccc tttgattcaa aatgatggtg cttatttgct gtgcaaaatg
    10921 gccgacgatc gcggcgtttt ccccggtcaa tgggcgcttt cgggtggcgg cgtggagtct
    10981 ggcgaacgaa ttgaagaggc actacgccgc gaaattcgcg aagaactggg agaacagctg
    11041 cttttgacag aaatcacgcc gtggaccttc agcgatgata ttcgcaccaa gacatatgca
    11101 gatggtcgca aggaagagat ttatatgatt tacctgattt ttgactgcgt ttctgccaac
    11161 cgagaagtga aaataaacga agagtttcag gactacgcgt gggtaaaacc tgaagatctg
    11221 gtgcattatg atttaaatgt tgccacccga aaaacgttac gtttgaaagg tcttctttaa
    11281 caatgatgac agccatcact ggctgtcaga ttgaaaattt agtggttaac gaattcccca
    11341 tccagataaa ctttgccttt ttccacatgc atgactaaac catccagata atcaggttta
    11401 aatatcgcat cacgcttatt tttagcaata tatgtcaggc aatgattatg ggtgaaaata
    11461 acgatatttt tatcaggcgc tttgctttgt aagtccttaa ttgcactata aatctcatta
    11521 ccgcactgaa gaagtcgttt atctaccgtc agctttttac ccgcggaaaa ccaggtcgcc
    11581 gactgaatgg tccggacggt attactggaa taaagatcga aatcagggat gtcagcgcta
    11641 aaagcgttgc ccagttcacg ggcatcctgg gtacctttaa ccgtaatacc tgttttatct
    11701 gacaagcatt gattggttga acgatcgcaa cgttcagcat ggcgaaacaa aacgacaacc
    11761 gggtgctgct gtgccagtct ggccaatgtt ttattgtcga tacgtggcaa accattgcta
    11821 ctccaggcgg catgagtacc cagtccggca attgcagcga gcgccagtaa aatgatgaaa
    11881 tatttttttg acttcaatga tgagcggcaa aaagctaaca cgtaaactcc acctatagac
    11941 aagcgcaacc agacaattac cgtgaaattg agcgacattt ctggcgataa ttagcagttg
    12001 gtgtaatatt aaaaatccta cgatgtcggc aaaatgcctc aaatttttgc caaatgcaaa
    12061 gcctaaataa gaaaaaatat aaaaatttca atatttacgt ctaatattag tttcttaagg
    12121 ttaagttaat attctatcct taaaatttcg ctccaaatgg caaaatatac acaacactct
    12181 ttatagcaaa tataagtgga caggtattca atggcggaag gaaaagcaat gtcagaattt
    12241 ttgccttttt cgcgaccagc aatgggcgtg gaggaactcg ctgcagttaa agaggttctc
    12301 gaatccggtt ggatcacaac cggtccgaag aatcaggcgc ttgagcaagc cttttgccag
    12361 ttgacgggaa atcagcatgc catcgcggtc agttcagcca ccgccggaat gcatatcacg
    12421 ctaatggcgt tggaaattgg caagggcgat gaagtgatta cgccttccct gacctgggtt
    12481 tcaaccctca atatgatttc cttgctgggt gcaacgccgg taatggtgga tgtcgaccgc
    12541 gatacgctga tggtcacgcc tgaagcgatc gaatcagcca ttacgccacg cactaaagcc
    12601 atcattccgg tgcattatgc cggtgcgcca gcagatattg acgccattcg cgccattggc
    12661 gaacgttacg gcatcgcagt tatcgaagat gctgcccatg ccgtcggtac gtattacaaa
    12721 gggcgacata ttggcgcaaa aggtaccgct attttttcat ttcatgccat taaaaatatt
    12781 acctgtgctg aaagtggcct gattgtaact gataatgaaa accttgcccg ccagctacgg
    12841 atgctgaaat ttcacggtct gggtgtcgat gcctatgaca gacaaacctg gggccgtgca
    12901 ccgcaggctg aagtcttaac accgggctat aagtacaatc tgaccgatat taacgccgcg
    12961 attgccctga cacagttagt caaattagag cacctcaaca cccgtcggcg cgaaattgcc
    13021 cagcaatatc agcaagcact ggcagctctc ccctttcagc cattaagcct tcccgcctgg
    13081 ccgcacgttc acgcctggca tctgtttatt attcgtgtcg atgaacaacg ttgtggtatc
    13141 agtcgcgatg cgttgatgga agcgttaaaa gaaagaggca ttggtaccgg gttacatttc
    13201 cgcaccgctc acacacaaaa atattatcgc gagcgttttc ccacgctgtc gttaccgaat
    13261 accgaatgga atagcgaacg catctgttcg ttgccgctgt tcccggatat gactaccgcc
    13321 gatgccgacc gcgtcatcac agcccttcag caactcgcag gacaataagc catgtttgaa
    13381 atccaccctg ttaagaaagt ctcggtggtt attcccgttt ataacgagca ggaaagctta
    13441 ccggaattaa tcaggcgcac caccacagcc tgtgaatcgt tggggaaaga gtatgagatc
    13501 ctgctgattg atgacggcag tagcgataat tccgcgcata tgctggtcga agcctcacaa
    13561 gcggagaaca gccatattgt gtctattttg attaaccgca attacgggca acattcagcg
    13621 attatggcgg gattcagtca cgttactggc gacttaatta ttacccttga tgccgatctc
    13681 cagaatccgc cagaagaaat cccccgcctg gtggcaaaag ccgatgaagg ttacgacgtg
    13741 gtagggactg tacgccagaa ccgccaggac agctggtttc gtaaaacagc ttcgaagatg
    13801 attaaccggc ttattcagcg caccactggc aaagcgatgg gtgactacgg ttgtatgctg
    13861 cgcgcctatc gccgtcatat tgtcgatgcg atgttgcact gccatgaacg cagcaccttt
    13921 atcccgattc tggcgaatat cttcgcccgc cgtgcaattg aaattccagt acatcatgcc
    13981 gagcgtgagt ttggtgaatc caaatacagt tttatgcgcc tgattaattt gatgtacgac
    14041 ctggtgacct gccttaccac aacgccgcta cgtatgctga gtctgctcgg cagcattatt
    14101 gcgattggag gttttagcat tgcggtgctg ctggtgattt tacgcctgac cttcggacca
    14161 caatgggcgg cagaaggcgt ctttatgcta tttgccgtgc tgtttacttt tattggcgct
    14221 cagtttatcg gcatgggatt actcggtgaa tatatcggca ggatctacac cgatgtccgc
    14281 gcccgccccc gctattttgt tcagcaagtt atccgtccat ccagcaagga aaatgaataa
    14341 tgaaaaccgt cgtttttgcc taccacgata tgggatgcct cggtattgaa gctctgctgg
    14401 ctgccggtta cgaaattagc gccattttta cccataccga taatcccggt gaaaaagcct
    14461 tttatggttc ggtggctcat ctagcggcgg aaagagacat tccggtttat gcgccggata
    14521 acgttaatca tccgctgtgg gtggaacgca ttgcccaact gtcaccagag gtgattttct
    14581 ctttttatta tcgccatctt atttgcgacg agatttttca gctcgctccc gcaggtgcat
    14641 ttaatctgca cggttcgctg ttaccaaaat atcgtggtcg cgcgccgctg aactgggtgc
    14701 tggtgaacgg tgaaacggaa acgggcgtta cattgcaccg aatggtaaaa cgtgccgatg
    14761 ctggggccat tgtggcgcaa ctgcgcgttg ccattgcgcc agacgatatc gctattacgc
    14821 tgcatcataa attgtgccat gccgcgcgcc agctactgga acagacatta cccgccatta
    14881 aacacggtaa tattctggaa atcgcccagc gcgaaaacga agccacctgt tttggtcgca
    14941 gaacgccgga tgacagtttc cttgaatggc ataaaccggc atccgtactg cacaacatgg
    15001 tacgtgccgt tgccgatccg tggccgggtg ccttcagcta tgttggcaat cagaaattca
    15061 ccgtctggtc gtcgcgtgtt catcctcatg ccagcaaagc acagccgggg agcgtgattt
    15121 ctgttgcgcc actgctgatt gcctgtggcg atggcgcgct ggaaatcgtc accggacagg
    15181 cgggcgacgg cattactatg cagggctcgc aattagcgca gatgctgggc ctggtgcaag
    15241 gttcacgctt gaatagccag cctgcctgca ccgcccgacg ccgtacccgg gtactcatcc
    15301 tcggggtgaa tggctttatt ggcaaccatc tgacagaacg cctgctgcgc gaagatcatt
    15361 atgaagttta cggtctggat attggcagcg atgcgataag ccgttttctg aatcatccac
    15421 attttcactt tgtcgaaggc gatatcagta ttcattccga atggattgag tatcacgtca
    15481 aaaaatgtga tgtcgtcttg ccgctggtgg cgatagccac gccgattgaa tatacccgca
    15541 acccgctgcg cgtatttgaa ctcgattttg aagagaatct gcgcattatc cgctactgcg
    15601 ttaagtaccg taagcgaatc atcttcccgt caacttcaga agtttatggg atgtgtagcg
    15661 ataaatactt cgatgaggac cattctaatt taatcgtcgg cccggtgaat aaaccacgct
    15721 ggatttattc ggtgtcaaaa caattacttg atcgggtgat ctgggcctat ggcgaaaaag
    15781 agggtttaca gttcaccctc ttcctcccgt ttaactggat gggaccacga ctggataacc
    15841 ttaatgcagc gcgaattggc agctcccgcg ctattacgca actcattctc aatctggtag
    15901 aaggttcacc aattaagcta attgatggcg gaaaacaaaa acgctgcttt actgatatcc
    15961 gggacggtat cgaggcgttg taccgcatta tcgaaaatgc gggaaatcgc tgcgacggcg
    16021 aaattatcaa cattggcaat cctgagaacg aagcgagcat tgaggaactg ggcgagatgc
    16081 tgctggcgag cttcgaaaaa catccgctgc gccatcattt cccaccgttt gcgggctttc
    16141 gcgttgtcga aagtagctgc tactacggca aaggatatca ggacgtagag catcgtaaac
    16201 cgagcatccg caatgcccac cgctgcctgg actgggagcc gaaaattgat atgcaggaaa
    16261 ccatcgacga aacgctggat ttcttcctgc gcaccgttga tcttacggat aaaccatcat
    16321 gaccaaagta ggcttacgca ttgatgtcga tacctttcgt ggcacccgtg aaggcgtgcc
    16381 gcgtctgctg gaaatcttga gtaagcataa tattcaggcc agcatttttt tcagcgtcgg
    16441 cccggacaat atgggccgcc atctctggcg actggtgaag ccacagtttt tgtggaagat
    16501 gctgcgctca aacgcggcat cgctttatgg ctgggatatt ttactggcag gtacggcctg
    16561 gccaggtaaa gagattggtc atgccaatgc cgatatcatt cgtgaagcgg ctaaacatca
    16621 cgaagtcggt ctgcacgcct gggatcacca tgcctggcaa gcccgtagcg gtaactggga
    16681 tcggcaaaca atgatcgacg atattgcacg cggtcttcgc actctggaag agattatcgg
    16741 tcaaccggta acctgttctg ccgctgcggg ctggcgtgcc gaccagaagg tgatcgaagc
    16801 aaaagaagcg ttccatttgc gctacaacag cgattgtcgt ggggccatac cgttccgtcc
    16861 actgcttgaa tcaggaaacc ctggcactgc gcaaattccg gtgaccttac ccacctggga
    16921 tgaagtgatt ggtcgggatg tgaaagcaga agattttaac ggttggttac tcaaccgcat
    16981 cctgcgagat aaaggcacgc cggtttatac cattcatgca gaagttgaag gctgcgctta
    17041 tcagcataat tttgtggatc tcctcaaacg cgcagctcag gaaggcgtga cattttgccc
    17101 tttaagcgaa ctgttatcag agacgttgcc gctcggacaa gttgttcgcg gaaatattgc
    17161 cggacgtgaa ggctggctgg gttgccaaca aattgcgggt agtcgctgat gaaatcggta
    17221 cgttacctta tcggcatctt cgcgtttatt gcctgctatt acctgttacc gatcagcacg
    17281 cgtctgctct ggcaacccga tgaaacgcgt tatgcggaaa tcagtcgaga aatgctggca
    17341 tccggcgact ggattgtgcc ccatctgtta gggctacgtt atttcgaaaa acccattgcc
    17401 ggatactgga ttaacagcat tgggcaatgg ctatttggcg cgaataactt tggtgtgcgg
    17461 gcaggcgtta tctttgcgac cctgttaact gccgcgctgg tgacctggtt tactctgcgc
    17521 ttatggcgcg ataaacgtct ggctctactc gccacagtaa tttatctctc attgtttatt
    17581 gtctatgcca tcggcactta tgccgtgctc gatccgttta ttgcattctg gctggtggcg
    17641 ggaatgtgca gcttctggct ggcaatgcag gcacagacgt ggaaaggcaa aagcgcagga
    17701 tttttactgc tgggaatcac ctgcggcatg ggggtgatga ccaaaggttt tctcgtcctt
    17761 gccgtgccgg tattaagcgt gctgccatgg gtagcgacgc aaaaacgctg gaaagatctc
    17821 tttatttacg gctggctggc ggttatcagt tgcgtactga cggttctccc ctggggactg
    17881 gcgatagcgc agcgggagcc tgacttctgg cactattttt tctgggttga gcatattcaa
    17941 cgctttgcac tagatgatgc ccaacataga gctccgttct ggtactacgt gccggtcatc
    18001 attgccggta gcctgccgtg gctgggatta ctccccggtg cactgtacac aggctggaaa
    18061 aaccgcaagc attccgcaac cgtctatttg ttgagctgga cgataatgcc gctgctgttt
    18121 ttctccgtcg ctaaaggcaa attgcccacc tatattcttt cttgctttgc acctctggca
    18181 atgctgttgg cgcattacgc gttgctggca gcaaaaaata atcctctggc gctgcggatt
    18241 aatggctgga ttaacatcgc ttttggcgtc actggcatta ttgccacatt tgtgatctcc
    18301 ccctggggac caatgaacac gccggtgtgg caaaccttcg agagctataa agtcttttgt
    18361 gcctggtcga ttttttcgct atgggcattt ttcggctggt acaccttaac aaacgtcgaa
    18421 aagacctggt ctttcgccgc gctttgcccg ctggggctgg cgttgctggt aggattttca
    18481 attcctgaca gagtcatgga aggaaaacat ccgcaatttt ttgtcgagat gacacaagaa
    18541 tcacttcagc caagccgcta tattcttact gatagcgtcg gtgttgccgc aggtctggca
    18601 tggagcctgc aacgcgatga catcatcatg tatcgccaga caggtgagtt gaaatacggc
    18661 cttaattatc cggatgcgaa agggagattt gtcagcggtg atgagttcgc aaactggctt
    18721 aatcaacatc gtcaggaggg gattattaca ctcgtgcttt cggttgaccg cgatgaagat
    18781 atcaacagtc tcgccattcc gcccgcagat gccatcgttc gtcaggagcg tctggtgctg
    18841 attcagtatc gtcccaaatg atctggctaa cattagtctt tgccagcttg cttagcgttg
    18901 ccgggcagtt gtgtcagaaa caggcaacct gctttgtggc gataaacaaa cggcgcaaac
    18961 atatcgtgct gtggctggga ctggcgctgg cttgtcttgg tcttgccatg atgctctggc
    19021 tgctggtctc gcagaacgta ccggtaggca ttgcctaccc gatgttaagt ctgaattttg
    19081 tctgggtgac gctggctgca gtaaaactgt ggcacgaacc ggtatcgccg cgtcactggt
    19141 gtggggtggc gttcattatt ggtggcattg tgatcctcgg gagtacggtg taatgggcct
    19201 gatatgggga ttattcagcg tcattattgc ctcagttgcg caattaagcc tgggttttgc
    19261 ggcaagtcat ctgccgccga tgacgcactt atgggatttt atcgcggctc tgttagcttt
    19321 tggcctggat gcccggatac tgttactcgg gctgctgggt tatctgcttt ccgttttttg
    19381 ttggtataaa acgctacata aacttgcgct aagcaaagcc tatgccttgc tcagtatgag
    19441 ttatgtgctg gtatggattg cttcaatggt acttcccggc tgggaaggga ctttttcgct
    19501 gaaagcacta ctgggagtag cctgtattat gagcgggttg atgctgattt tcctgcccac
    19561 gacaaaacaa cgttactgag ttttccctgc cgctgtacac tgccgttccc actcatcggg
    19621 gctataactg cttgccgaga ggactttcac cgtatgcagc ttttcacgat taatacaata
    19681 aagcgcattc tgcaaaggcg agagtaaatc ccccactttc acggcgaagt cactcttcac
    19741 ttcggcgatc atttttatcg cgccgccagc atcgcagagc ataagtacat gtctgttatc
    19801 ttgtttattg cagcacgatt ttttgtgcca gccattccat tgcattatcc tgtttgctaa
    19861 gagttttcac atcaatttac agattaacag ataaactctt tatcaacggc aatggaatgg
    19921 cagaaatgag aagtgaaacg gagggttaat tattgctgac gttgcaccca ctcttttagc
    19981 gcctgacgtg aaattttaat accgccgttt ttcagctccg gcggtaaagt cagccagcgc
    20041 accggttgtt gaaaacgagc cagcttatct ttcacccatt cgccaagatc aacggtttgc
    20101 tggtcatact ccaccaccgc taccggccga tgaccaaact ccttgtcggc aacggaaacg
    20161 ataaacacct gcaaaaccgc aggatgtgca gcaataacac gctcgacttc ttccggctga
    20221 ataccctcac cgccactgaa gaacagattg tctaaacgtc cgacaatggt cagcttgcca
    20281 ttatgcatct caccgcgatc gcgcgtagcg taccagcctt cgtcattaac cagtgaaacc
    20341 agttgcccgt tacgccagta accttctgcc atactggcag cccgcagcca cacttcatta
    20401 ttaacgattt tcacttcccg acccggcagc ggcaaaccaa cgtctgccag gccgtcggct
    20461 tctttcgcac ataccgtgga ggcaaactcg gtcagaccat agccgcaaaa gcaacgaatc
    20521 ccctgctcgc gcgcctgttc cgtcaactcg accgggatag ccgcgccgcc aagtaacacc
    20581 gctttcaggg aaacggaact gcggttaacc agcaaacgcc agagttgtgt tggcaccagt
    20641 gaagcgtgag tacagcctgc cagcatttgc tccaatggct gtttatcacg caccgtcatc
    20701 cgcgcaccag cgtataacca gcgccacata attccctgac cggagacgtg aaacagcggt
    20761 aaagagagca accagtcatc gtgatcgcca aacggaatca gcgataacac accttgcgca
    20821 ctggcaagat gggcctgata agtatggaca gcggctttcg gcaaaccggt agaacccgag
    20881 gtcaacgtca ttgagcacag acgcgtcggc tgccacgcag cggcatgtac gccttcaacc
    20941 agctgaatgt gcagcgacgt taatgccgga aacgtgtttt ccccatccgg caccagcgca
    21001 aattgcagcg tcagattggg cagcaattct tcaagcaacg gttgcggcag ctgagggttc
    21061 acgggcaaca cccgcgcccc gcattgcagt aacgccagcc aggcgagcag cgtttgcggc
    21121 gtattccacg cccgcaacat cacgccgctg ccctcaacca ccccctgcgc cgcaaagccc
    21181 gtagctaact catcgacgcg agcacaaagc tcgcgccagt tgagttgctc gtcattaaga
    21241 cgtaaggcga tggcttctcc ccgcacttgc cgccagtgac gccacggcca gtcagagaag
    21301 atcataacaa ctgctcctgt acatcagcgt ccactaacgg cagtgggctc cccggccagc
    21361 gacgaacctg ctgcgcctgc atcagatcca gcgtgtccag tcccggaatg gtgtctggcg
    21421 ttaaccaggc agcaatccgc gccagttgcg ttagacctaa gctcgactca atggaagaac
    21481 tgatcaccgc cgtcagcccc agcgcatgcg ccgcctgtac ctgctcgcgt actttttcca
    21541 gactgcccgt gagcgtgggt ttgataacta ccgcgcgcac gccctcttca gccacaaagg
    21601 caaaatccgg ctcgcgcagg ctttcatccc aggcaatggc aatgccggtt tcacgggcaa
    21661 acgctcgcga atcatcgcgg gttttgcacg gctcttcgag aaacgcgatg cggtggcgat
    21721 aatccgggtt aacatatttg gcaaactgct gacctttcag cggtgtccag gcgcgatttg
    21781 cgtcaagacg caaatgcaga tccggaattg cctccagcaa cagattcacc accatgccgt
    21841 cgcgcaccgc ttcgtacaat cccactttga ccttcgccac tttctcgccc ggcatatcgg
    21901 caagtttgag gatcagatcg tccggatcgc cattacacag cggtgccgca cggtagttgg
    21961 ctgcttgcgg caacgtatct gtcagttctg ccaatgcaca gcttacgcca aaggccacgg
    22021 aaggcatctg cggtagctcg caatcgcctg ccagccagtt atttacccag gcaagcagca
    22081 cgctttgcgc ctcttcccag ttttcctggc tgaagcccgg cagtggggag atctcccccc
    22141 acccttcgcg ctcgccctca cgcaggcaaa cgtacagccc gtcgcgggtt tttaaccgcc
    22201 tgtcgcgcag aaccaccccc gcgtccatgg ggatctgcca gcggtatacc tgcgcgctac
    22261 gcattacgga ttccgtttaa atttgctgaa atcaggctga cgtttctggt tgaaagcgtt
    22321 gcgaccttcc tgaccttctt ccgtcatgta gaacagcatg gtggcgttgc ccgccagctc
    22381 ctgcagcccc gcctgcccgt cacagtcggc gttcagcgca gctttcaggc agcgcagcgc
    22441 catcgggctg ttttgcagca tttcgcggca ccaacggacg gtttcttttt ccagatccgc
    22501 cagcggtacc acggtgttga caaggcccat atccagcgcc tgttttgcgt cgtactgacg
    22561 gcacaggaac cagatttctc gcgctttttt ctgcccgacg atacgagcca tgtaggaagc
    22621 gccccagccg ccgtcgaagg aaccgacttt cgggccagtc tgaccgaaga tggcattatc
    22681 tgctgcgata gtcaggtcgc acatcatgtg cagaacgtga ccaccgccga tggagtagcc
    22741 agccaccatc gcgacaaccg gtttcggaca ggtacggatc tgacgctgga agtccagcac
    22801 gttcaggtga tgtacgccgg aatcatcttt atagccgccg taatcaccac gcactttctg
    22861 gtcaccaccg gagcagaacg ctttatcgcc tgcgccagtc agaatgatca cgccgatatt
    22921 gtcgtcataa cgcgcatctg ccagtgcctg gatcatctct tttaccgtca gaggacggaa
    22981 ggcattgcgc acctgcggac gattaatggt gatttttgcg ataccgtcgg tggatttttc
    23041 ataacgaatg tcctcgaaac cttcggagca gtcgtgccat tcaaccggtg cgtaaggcat
    23101 tgcttcatca ggataaatca tattgtgtcc tttagtcaga aacgcaagat ctgcgccaga
    23161 cttgcgatta cgccagcggg attttcccga tgcgcgttat gtccggcgcg aggaatgaca
    23221 tggcagtcgg cagccagttc cgccgccagg gcgcggaatt tgctgtcacg ttcaccacat
    23281 aaataataaa acgcaaatgt gcgggcgcta aggttagcac gtaaatcagg ctggacggcg
    23341 agagaagtcg cctccagcat ggcggcaagg gttgcgccat tattgttgct gcgcagcgcc
    23401 accagctccc ggcgttgatc gtcattgagc gaggcaaaaa caggctgttg ataccagtcg
    23461 gcaaataccg ccgttaacgg ttctgtgcga aaacgctgcg cccattggcg atcggaacgc
    23521 tgacgttccg cacgttgttc agcattttgc agccccggat gcccgccttc gacaataacc
    23581 ccacaaagcc ccgccagccc ctggcaagcc gccatcatcg ccacccgtcc gccaagcgag
    23641 taccccacca gccagaagtc aaggatgttg taactaacca aggttttacg cagtaagtcg
    23701 gtgacatcat caaatccatc gacgctaatc gccgccgaac caccatgacc taggagatca
    23761 acatacaacc gtgagtagtc ggcaaacgcc tcgcccactt cttgccattc gtggcaatcg
    23821 ccggaaaaac cgtgcaaaaa caccagccag ggtaaaccta gttttccgtg ttttgcctgc
    23881 gcgtgcagga tcataaatgg cttacctgcg ccagaagttg ctggagcgtt tgcgcaccat
    23941 cggtgtcgtt aaccaccatt tcaatcaccg tggtggttgg cgtgcgccag gcgtcggcaa
    24001 atgccgtttc aagttcctgc cagttttgcg gatgatgata tttcagctcg aacatcgcgg
    24061 cggcgtgctc aaaatggacg ttttgcggca tcagatagaa acgctcacgc tcgctttgcg
    24121 gcgttggcaa cagcgagaaa atttgcccgc cgttgttgtt caccacaatt aataccagcg
    24181 gcgcagaaac ctgacgcaat aacgccagcg cgttgagatc gtaaagcgcg gagagatcgc
    24241 ccacaatcgc cagcgtcggt ttgccgcttg cccgctgaac gcctgcggcg gtcgaaagca
    24301 gtccgtcgat accgctggcc cctcggttgc tgtacaccgg gtaacctgcc ggaagttgcg
    24361 aaagcgcatc aatcagacgt accaccaggc tgttaccaac aaacaattgc ccctgttcag
    24421 gcagatagtc gctgatgcga tgcgccagtt gcgcttcgcc aaacgcatca cggcgggcaa
    24481 taaccgcctg cattgcctgt tccgccaggc gcgggatttc aacgcaccag ggctggcgtt
    24541 tttctgccgg atgcagctcc agccagtcgg caatattggc aattaagcga cgtccgcgat
    24601 ggtgtgccgg atcaagtcgc ccttcaatgt catcaacaat ccagtactct tctggttcac
    24661 agcttgcctg ccattgcagg agccgtttcc ccgtcaggct acttcccagt tgcaccacaa
    24721 tttgcgcctg ctgcagctcg ctggtcgctt tggcattgcc taaccaaaga tcggcacacg
    24781 gcagcggctg cccggtttgt gacagcacat cgccaatcag cggccagcca agagtttgcg
    24841 cccacagggc aacttttttg ccctcttccg cactcatgcg cccggcaacc accacgccgc
    24901 gcttttgtcg ccagaagaac cagtcgcgct gtttttcact ttccagacga ggcgcttcac
    24961 gcagccacgg tttgtcgtcc tgccaccagt cacccagacg ctgttgccag ctaagcccgg
    25021 tatcgtccat ttcgccatac agcggttcag caaacgggca gttgatatgg actccccccg
    25081 catgaagcgt accgagagcg tggtcgatgg tagaaaccag ccaacgtgcg gggatatcct
    25141 gggtcgggcg cggcaatgaa atactgtgcg tggggtgaga ggcgaacatt cccggctggc
    25201 gaattgcctg attcgcgccg cagtcaatta gctccggcgg gcgatcggcg gttaagagaa
    25261 tcagtttttc tccggttaac ccggcttcaa tcagtgccgg atagagattt gccaccgccg
    25321 tgccggaggt cacaatcacc gccaccggct gcttgctgac tttcgccagc cccagcgcca
    25381 gatgccccaa cccacgctca tcgaaatggg tgtggtgaat gaatgcggaa ttctccgccg
    25441 ccgctaacgt taacggtgta gaacgcgagc ctggggcgat acagatgtgt ctgacgccgt
    25501 gacgcgttaa tgcttccaga atgaccgccg cccagcgtcg gttaaatgcg cttactgaca
    25561 tgagtttgtc cggtatcaat attgcggcta agtataagga gctacaaaaa tagattattg
    25621 atatgaatcg gtaatgatgc gactcattac tattccattt gtaataaagt acgcagccct
    25681 gccgctttgt tgtcgatttc ctgccactct tgctcggggt cggaaccacg gacaatgccc
    25741 gcgccagcat ataatcgcac gacattgccg ctaatttttg ctgagcgcag ggaaacgcag
    25801 aattcgcttt gttgtaatga gagatagccc gcagaaccgg cgtaccattc tcgggtgaac
    25861 ggttcgtgac gggcgataaa ctgtcgcgcc agatcgcgcg gtaagccagc aactgctgcc
    25921 gttggctgca actgatgtaa acagatcaca tcatccgctt tgttgagtga agtccagata
    25981 cagcggcgaa gatgctgcac tttacgcaga cgcagtacct gcggcggtaa aacatccagc
    26041 gtctgggtat cggcctgtaa tcgttggcag atatcttcca ccaccagcat gttctcgcgc
    26101 tggtttttat catccgccat cagccactct cctaactgct gcgcctgctt atcatcagga
    26161 tgatttgcta ctgttcccgc cagcgcttca gtacgcagcg ctttgtcacg ccgccgccat
    26221 aaccgttccg gtgaagagcc aagaaaagca ttttcgccat caaaggccat gtaaaaatgg
    26281 tagcaattca gattcagtcg acgactggca gccatcatcg ccgccgcgtt gaccggactt
    26341 gcgaaatgca ggtcagttgc ccgagcgagc accactttgt cgagctcacc ttcggcgatg
    26401 gttttcgttg ccagttcgat taattgcgtc cagcccgttt tgtccggcca gtgttgttct
    26461 cgcgtggtgg ttaaatgtaa cccaggcaag ggcttgatac tcaccagtgt ggcgataaat
    26521 tcttttgcct gaatcgcatc gtgctgaagg gagctttcgc tgaatagcgt cagccgcagc
    26581 gtggctttac cgccacagcg tcgccattcc aggcggggta aaagtaaatt gccctgcgac
    26641 gggtcaaaag cattcagccc ccaaatgcgt aagtcagcgt gttccgggtg ctggcgaaga
    26701 aaacgttgtg cctggtccaa cgacgtaaaa cgggtaatcg cgcccaggac ggcagcttct
    26761 tcatcaccat tacgttgttg ccagtagaat tgcgggtacg tttgctgact ggccagccag
    26821 ctcaaggcat caaaagcgtc tttgagaggg aaaggaatat cgataacccg aatgccgggt
    26881 gtcgccggaa tctcttgcga caaatggcgc agtagatttt ccagcgccgt agtaagtgat
    26941 tgcacgcgga cctctccctg ttaaaagcct cacattatac ggggtactac aaaaaaaatg
    27001 cagtaccccg gtgtagggag gttttaacgg cgtgccagca acagtcctag taccagcccg
    27061 acggccgcgc ccacaccaat tccttgccag ggtttttcgt ggacgtagtc atcagcacga
    27121 taaacagcct gcttcgcccg atagtaataa ctgtctgaag cctggctaac ccgttttttc
    27181 acatcatcca gcgctttttc tgcatgcgct ttcagctcaa catatttctg atcggcggga
    27241 tcgccagagg agcggagcac ctcttccagt gtttcactaa gcagcgtcag gtcgtcatcg
    27301 atacgtgtat caccaaactg attagacatt ctcgttctcc atttgcgtaa aacctgtcac
    27361 taactataga caagagttgt tgattacgcc tgaattacct cgcgtgccat cccaatgtgt
    27421 gggataccat cttcttcata gacctccgtc acagggataa aaccgaaact ctggtagaag
    27481 ttttgcagat gcgcctgcgc cccgaggtaa acaggcttat caggccagtg atgcgtacat
    27541 gtttcgagtg ttttactcat cagctgctgc cccacttttt caccacgcaa cgcttcactg
    27601 acaatcaccc gacctataac gaccggctca agatcatcat cacttttcag aatcctcgca
    27661 tacgccacca gttcatcatt tttccaaccg agaatatggc gattatcccc cgtcaaatca
    27721 tcaccatcga tatcctgata agggcaattc tgctcgacaa caaacaccgc gcaacgcagt
    27781 tgtaataagg cataaagctg ggaaaccgat aattcagagt gatgcagatc ttgccattca
    27841 atcataatgc tctcctttta gatgtatgcc ttatactagc cggaatattt tttgaaacgt
    27901 gatgaactca tggaattaat ttttttaggt acttcagccg gtgtgccaac ccgcacgcgc
    27961 aatgtcacgg caatattgct taacctgcaa catccgaccc agagcggact ttggttgttt
    28021 gactgcggtg aaggcaccca gcatcagcta ctgcataccg cctttaaccc tggaaaactg
    28081 gacaagattt ttatcagtca ccttcatggc gatcatcttt ttggtttacc cggcttgctg
    28141 tgcagtcgtt ctatgtcagg cattatccaa cccttaacga tttatggtcc gcaaggtatc
    28201 cgtgaatttg tggaaaccgc gctgcggatt agcggctcat ggaccgatta tccgctggaa
    28261 attgtcgaaa ttggcgctgg cgaaattctc gatgatggcc tgcgcaaagt aaccgcttat
    28321 ccgctggaac acccactgga atgttatggc tatcgtattg aagaacatga taaaccgggt
    28381 gcattaaatg cccaggcatt aaaagctgct ggcgtgccgc ctggcccact gtttcaggaa
    28441 ttaaaagcgg gcaaaacaat cacgctggaa gatggaaggc agattaacgg cgcagattac
    28501 ttagctgctc cagtgccagg taaagcgctc gctattttcg gcgataccgg cccctgcgat
    28561 gccgcacttg acctggctaa aggtgtcgat gtcatggtgc acgaagcgac gctggatata
    28621 accatggaag ccaaagccaa tagtcgcggc catagctcta cacgccaggc tgcgacacta
    28681 gcccgtgagg ctggagtcgg caagctaatc attacccacg tcagctcgcg ctatgatgac
    28741 aaaggttgtc aacacctgtt acgtgaatgc aggtcaattt tcccggcgac tgaactggcg
    28801 aatgatttca ccgtgtttaa cgtttaactc tgcttattat taaaaacagg gtgaaacttg
    28861 ccctgttata gcaacccgcg ctacatatat ccacccacac aggcatattg agcgattttt
    28921 gatatttatt catctcctta cgcactatat tcagagcaat aagttgcctc ttttcatctt
    28981 gattcagcac aacctttgca actaaaagga gcccatgatg gttacagttg tcagcaatta
    29041 ttgtcaatta tctcaaaccc aactcagtca gacatttgca gaaaaattta ctgtgaccga
    29101 ggaattactg cagtctttaa aaaaaacagc gttatccgga gatgaagaaa gtattgagtt
    29161 actgcataat attgcgttag gttatgatga atttggaaaa aaagctgggt aatgctgcca
    29221 acttactgat ttagtg
//
LOCUS       DAEEUE010000044        29197 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.044, whole genome shotgun sequence.
ACCESSION   DAEEUE010000044 DAEEUE010000000
VERSION     DAEEUE010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29197
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.044"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(177..1073)
                     /gene="yhaJ"
                     /locus_tag="KLK84_002109"
     CDS             complement(177..1073)
                     /gene="yhaJ"
                     /locus_tag="KLK84_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HBE2009428.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTVEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            1178..1879
                     /locus_tag="KLK84_002110"
     CDS             1178..1879
                     /locus_tag="KLK84_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HBE2009429.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1902..2066
                     /locus_tag="KLK84_002111"
     CDS             1902..2066
                     /locus_tag="KLK84_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009430.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2278..3588)
                     /locus_tag="KLK84_002112"
     CDS             complement(2278..3588)
                     /locus_tag="KLK84_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBE2009431.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3616..4947)
                     /locus_tag="KLK84_002113"
     CDS             complement(3616..4947)
                     /locus_tag="KLK84_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HBE2009432.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(5222..6586)
                     /gene="tdcG"
                     /locus_tag="KLK84_002114"
     CDS             complement(5222..6586)
                     /gene="tdcG"
                     /locus_tag="KLK84_002114"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBE2009433.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6658..7047)
                     /locus_tag="KLK84_002115"
     CDS             complement(6658..7047)
                     /locus_tag="KLK84_002115"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HBE2009434.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7061..9355)
                     /gene="tdcE"
                     /locus_tag="KLK84_002116"
     CDS             complement(7061..9355)
                     /gene="tdcE"
                     /locus_tag="KLK84_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HBE2009435.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9389..10597)
                     /gene="tdcD"
                     /locus_tag="KLK84_002117"
     CDS             complement(9389..10597)
                     /gene="tdcD"
                     /locus_tag="KLK84_002117"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HBE2009436.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10623..11954)
                     /gene="tdcC"
                     /locus_tag="KLK84_002118"
     CDS             complement(10623..11954)
                     /gene="tdcC"
                     /locus_tag="KLK84_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HBE2009437.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11976..12965)
                     /gene="tdcB"
                     /locus_tag="KLK84_002119"
     CDS             complement(11976..12965)
                     /gene="tdcB"
                     /locus_tag="KLK84_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HBE2009438.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13064..14002)
                     /gene="tdcA"
                     /locus_tag="KLK84_002120"
     CDS             complement(13064..14002)
                     /gene="tdcA"
                     /locus_tag="KLK84_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HBE2009439.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            14192..14536
                     /gene="tdcR"
                     /locus_tag="KLK84_002121"
     CDS             14192..14536
                     /gene="tdcR"
                     /locus_tag="KLK84_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HBE2009440.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            14792..15331
                     /locus_tag="KLK84_002122"
     CDS             14792..15331
                     /locus_tag="KLK84_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009441.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTPPRHFNKTCIT
                     SDCWQILFFSPDHFNNDFY"
     gene            15353..15700
                     /locus_tag="KLK84_002123"
     CDS             15353..15700
                     /locus_tag="KLK84_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009442.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            15869..16519
                     /locus_tag="KLK84_002124"
     CDS             15869..16519
                     /locus_tag="KLK84_002124"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009443.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNCLNRIKLPEMKEFSSKKIYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
     gene            complement(17140..17516)
                     /gene="rnpB"
                     /locus_tag="KLK84_002125"
     ncRNA           complement(17140..17516)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="KLK84_002125"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(17549..18694)
                     /gene="garK"
                     /locus_tag="KLK84_002126"
                     /pseudo
     CDS             complement(17549..18694)
                     /gene="garK"
                     /locus_tag="KLK84_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
     gene            complement(18791..19681)
                     /gene="garR"
                     /locus_tag="KLK84_002127"
     CDS             complement(18791..19681)
                     /gene="garR"
                     /locus_tag="KLK84_002127"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBE2009444.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(19711..20481)
                     /gene="garL"
                     /locus_tag="KLK84_002128"
                     /pseudo
     CDS             complement(19711..20481)
                     /gene="garL"
                     /locus_tag="KLK84_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(20497..21831)
                     /gene="garP"
                     /locus_tag="KLK84_002129"
     CDS             complement(20497..21831)
                     /gene="garP"
                     /locus_tag="KLK84_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HBE2009445.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGLKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            22206..23777
                     /gene="garD"
                     /locus_tag="KLK84_002130"
     CDS             22206..23777
                     /gene="garD"
                     /locus_tag="KLK84_002130"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HBE2009446.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVEIASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(23829..24653)
                     /gene="agaR"
                     /locus_tag="KLK84_002131"
     CDS             complement(23829..24653)
                     /gene="agaR"
                     /locus_tag="KLK84_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HBE2009447.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            24902..26182
                     /gene="kbaZ"
                     /locus_tag="KLK84_002132"
     CDS             24902..26182
                     /gene="kbaZ"
                     /locus_tag="KLK84_002132"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HBE2009448.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            26205..26678
                     /gene="agaV"
                     /locus_tag="KLK84_002133"
     CDS             26205..26678
                     /gene="agaV"
                     /locus_tag="KLK84_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HBE2009449.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            26689..27468
                     /locus_tag="KLK84_002134"
     CDS             26689..27468
                     /locus_tag="KLK84_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HBE2009450.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            27458..28267
                     /locus_tag="KLK84_002135"
     CDS             27458..28267
                     /locus_tag="KLK84_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBE2009451.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            28354..28788
                     /locus_tag="KLK84_002136"
     CDS             28354..28788
                     /locus_tag="KLK84_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBE2009452.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            28785..>29197
                     /locus_tag="KLK84_002137"
     CDS             28785..>29197
                     /locus_tag="KLK84_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBE2009453.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         7593 a         7623 c         6891 g         7090 t
ORIGIN      
        1 attgttgaca aagtgcgctt tgtttatgcc ggatgcggcg taaacgcctt atccagccta
       61 caaattgcca aaattcaata tattgcagtg actgcgtagg cctgataagc gtagcgcatc
      121 aggcagtttt gcgtttgtca gcagtcagaa gcaccttgtg aaaggtgctt aatagattat
      181 tttccagcga acaactttgg aatttcccgc agacaccagg atttcgcttc ccccatactg
      241 tcacgacgcc aggccataat aatatcgatc tcgctggtcg attccgggct gacgacacgc
      301 aaccgccctt ccgcaatatc tttttcgacc atcggatacg gcatcgtcgc cacgccaagc
      361 cccgccagta atgcctgacg tttatcttca accgtgctca ccgttaagcg cggctgtttg
      421 tccagcagct gtacggtcaa caccgggcgc tcacgagcgg tatccgctac cgcaattcca
      481 cgatatttca cacgcgtcac ttcagataac ggttccggct cctgatgaat cgggtgatca
      541 ggcgcggcaa cgtagacgtt cattaacgta tagagcttgc gcgagttgat ctccgacgag
      601 gaacgaaaat gcatatccgg cgcgataacg atatccgccc gcccctgctc cagccgttcc
      661 cacgcccccg ccagcacttc tgtgatgatt gccagttggg tattggcttt tgccgccagt
      721 ttgtcgatta acgggaaaaa ggcaggtgtc ggtaccagcg cttcggtcac aatggtgaga
      781 tgcgtttccc aaccgcgcgc gagagcttcc gcatcagtag tcagtttatc tgcggcttcc
      841 agcaaaacgc gccctcgctc cagcaacatc ctcccgacat tggtgaattt ggtgcgatgg
      901 cccgagcggt caaacagcac cacatccagc tcttcttcca gtttttgcat ggtgtagcta
      961 agtgcggaag gcacgcgtcc cagctcatcc gctgccgccg caaaactgcc ccggcgatcg
     1021 atcgcatcca taacccgtag tgcttccagc gttaatgccc tttctttggc catttcgttc
     1081 tcattcagga aatttgaaca taccgggcag aatatctggc taacaatgta gcgtccagcc
     1141 ccttaccata aaaggaagta aagagaggtc aagaattatg attactaccc gaactgccag
     1201 gcagtgtgga caagcagact acggatggtt gcaggcccgg tatacttttt cctttggaca
     1261 ctacttcgac ccgaaattgt taggctatgc ctccctgcgt gtgcttaacc aggaagtgct
     1321 ggccccaggt gccgcctttc agccgcgaac ctatcccaaa gtcgatattt taaatgtgat
     1381 tctggatgga gaagcagagt atcgcgacag cgaaggcaat catgttcagg ccagcgccgg
     1441 agaggcgttg ctgctctcta cccagccggg tgtcagctat agcgaacaca atctcagcaa
     1501 agacaaaccg ttaacgcgaa tgcagctttg gctggatacc tgcccgcagc gagagaatcc
     1561 gctgattcaa aagctggcgc ttaatatgga caagcagcaa ttaatcgcct cgccagaggg
     1621 tgcgatggga agcctgcaat tacgccaaca agtgtggctg caccatatcg tgctcgacaa
     1681 aggcgaaagt gcgaatttcc agttgcatgg gccacgcgcg tatttgcaat cgattcacgg
     1741 gaaatttcat gcgcttacgc atcatgaaga gaaagcggcg ctaacctgcg gtgatggggc
     1801 gtttattcgt gacgaggcta acattacgct ggttgccgat tccccactgc gcgctttgct
     1861 gatagatttg cctgtctagt tgtttttaca gggagatgat gatgagtaaa aaatcgacca
     1921 aaaagcgcca gccagcgaag cccgtggtgg cgaaagaacc tgctcgcacc gccaaaaatt
     1981 ttggctatga agagatgttg agcgagctgg aagctatcgt cgcggatgct gaaacgcgtt
     2041 tagccgagga tgaagctacc gcgtaaagac aggttgccgg atgcggcgta aacgccttat
     2101 ccggcctaca aaacacaaca acatcaacaa attaaacacc ccgtaggccg gataagatgc
     2161 gccagcatcg tatccggcat caggtacaaa acacaacaac atcaacaagt tgagcacccc
     2221 gtaggccgga taaggtgcgc cagcatcgca tccggcacaa tactcaaaac ctggcgttta
     2281 tctggccttg ctcgccataa tctcgataat ctgccgatcc gtttgctgca tcgaatggct
     2341 tgctaacgca cacaggttgg caatcgactg ctcaacatca tgcgccacga tcccttcatt
     2401 gccggtcacg gcggtatcat ccagcgccat taacaccgct ttccacgcag ccgaagcact
     2461 ggtcgaaacc ttcatcgcgc agctgttcga cgcaccatcg caaatcatgc cgctgacatc
     2521 gccgatcata ctgctgatcg ccatcgagat agtttcataa cgcccatcca ccagccatgc
     2581 catcccggcg gcggccccca ttgctgcggt cgttgcggca caaagtgcag acaaacgcgg
     2641 taactggtta tggatgtaaa ttgcgctcaa atgagaaagc atcagcgcac gcgccagtcg
     2701 ttcatcatcc gctccgaagt gttctgctac aaccaccaca ggcattattg cagtgatccc
     2761 ctggttaccc gagccggagt tactcatagc cggaagcgta gcgccgccca tacgcgcatc
     2821 ggatgccgcg ctggtacgaa tcacaatgga tgaagagaga tctttcgcca gcaaaccgcg
     2881 ctcgcactgt ttttccagcg tcgcgccaat atgcagcccc cacttaccgc tcaaaccttc
     2941 ctgcgataac gcacaattta gcttcgcgga atcgagaata aagcggatcg ccgcaaatgg
     3001 gacttcattg acgaacttca ggatctcagc cagcgtcgtt ctggaaagca ccgtcagcgg
     3061 cgactcttgc tcgccctctg ccacacacgc ctggtgggta aacaccacac cattgtgcgt
     3121 ctcaatatgc acaatgttgg tatgcccgcc gacgatggtg acacacgccc acttctcacc
     3181 gttccagact ttggcgcgtg agaagaggat ttcattgcaa ggttcctgga tcttaacaga
     3241 gactttcccc gccgccagca gtgctttggc atcggcaatt gcctgcgcag ttgcgtcttt
     3301 cagcacttcc agcccggcgt tggcatttcc acctaacgcc cccagcgccg ccgcaatcgg
     3361 cagccccacc attcccgtgc cgggaacggt gacgcccaga ccgttcttca tcagatttgg
     3421 cgaaacccag gcttctacac gttcaaccgg accttccagt tctgccgcag caaccgccgc
     3481 cgccagcgcc agtgaaatcg gttcagtaca tcccagcgcc ggttttactt cctcctgaac
     3541 ggcgaggatg taacgctgcc ataacggatt taaagtcgaa tcaaacatat taaaaacctt
     3601 aaaatttcag gtaaatcaag aaaatgccag gaacagagaa acacaaagca acaaaccagt
     3661 gacgataatc aggtacagag acatcccttt gtatttgtgc aatgccggta ctttgtaaac
     3721 cagccacgcc gggatcaggc accctaccat gccgaaaatc gggctacaga tagaggtgaa
     3781 gctcaacacc ggtgcgttca gtacgatggc gctccaggcc agcaaaatgg cgaaaatcat
     3841 gatgccgcgc tgaacaagat tttcgttaat cttctcggca ggcatcttgc gacgcaggat
     3901 gttcattacg atcccttgcg ttgcttcgcg aaagcctaaa tagacgccaa agaatgcagt
     3961 catgacagca aagatattga gaatgacgct gaccactttc acccacgctg cgccgtcacc
     4021 gctaataaac tgtgcggcaa tcgccagcgc ggaaatattc tgctcgtagg cttttaccgc
     4081 ttcgtcatgt cccatcgcca gcgtgaacga cacggcgtag aaaaagacgg tgacaaacaa
     4141 aatgccaaac gcgatattca ttgcccgcaa tgctttatgc cgcgccactt caattgattt
     4201 ttcccgcgag cgataagaga tcaccatcgg gcttaacgtc tggataaaca gaatcgacgt
     4261 cagggtaaac ggcagcgtaa taatggcgtt tttcaccagc agccccagcg gcggtagcga
     4321 accgacgtta tacagatgcc acattcccac catcgacacg cccagcgcag cgactaccag
     4381 cagcttggtc agcaccatgt cggtcgaaat tttgaataac aatttctcgc cgcgtgaaga
     4441 gatcgccacc agaatgcaaa tcagtaccag accataaaag ggactgtctg acagcaaccc
     4501 ttccgtcacg ccgaaggtat gcagatagga agcactatcg ttggtgatgg cggtggaata
     4561 gacgaacatc caaatcacca gcattacgaa atagagcgca cctaacagga tgccccagtt
     4621 tttacctaaa taaccgctaa tgacactcgg gtaatcttta cattctggtg attctgccag
     4681 cgtattaata aacaaccgct gaaacagata cattgccggg taaccaatca ccgatgagag
     4741 caaaaatacc cacaatccca tcaaaccgac ctgcaccggg agaaaaacaa tccccgcgcc
     4801 aatcgccatc ccgatactca taatcaccca gccggtgtca gtactgtcga atttgatcgc
     4861 ttctcgccac tcgctctcac tcattccagc acgacccgcc ggggtcgaag cgtctgcaat
     4921 gacgccttta ttcgatgcaa tttccataat ttctcgctca atattttgta gggcttatta
     4981 tttttttccg agccgcatca aggcgatatg cggtctacgt ttttgcaggc gagttattag
     5041 aataaagaaa tgatacgcgc gaggtcagag aaaatcttca caatcaagac tttaaaataa
     5101 cggagatgga taagaatttt ctactaaatt aatcgcaaga gaatattcat tctctatttg
     5161 cggcagatca caaaaaaaag gtgcacattt gtgcacccaa ggatgaaagc tgacagcaat
     5221 gtcagccgca gaccacttta atggccagtc ctccgcgtga tgtttcgcgg tatttatcgt
     5281 tcatatcttt gccggtttca tacatcgtct cgatcacttt atcgagtgaa acacgcggtg
     5341 ccgaggtgcg gcgcatcgcc atccgcgcgg cgtttactgc tttcacggca ttaatggcat
     5401 tacgttcaat gcacgggatt tgtacctgtc cggcaaccgg atcgcaggtc agcccaaggt
     5461 tatgctccat cgcgatttcc gccgcattgc atacctgcgc cggactgccg cccagtagtt
     5521 cagttaaccc tgccgctgcc attgaacagg ccacgccaat ctccccctga cagccgacct
     5581 ccgcgccaga gatggaggcg ttcattttat acagcgcgcc aatagccccc gctgccagaa
     5641 aatagcgggc aattgaccgc tcgtttaccg gacgacggaa cttatcgtaa taagccagca
     5701 ctgccggaat aatgccgcac gcaccgttag tcggtgccgt taccacgcgc ccgccagctg
     5761 cgttttcttc actaaccgcc agcgcgtaca tgttgatcca gtcgatgaca ttcatcggat
     5821 cgttagagat gttatcgcta gaaaccagct gacgacgcag cgctacggca cgacacggca
     5881 cattgagcgg accaggcagc acgccttcag tgttcatccc gcgttcaata ccgtcatgca
     5941 tcacttgcca gatacgggca aaccctgcgt caatttccgc tttgctgcgc agcgccagct
     6001 cgttgtgcat catcaggcca gatatagaca ggccgttgta atcacacatt ttcagcagtt
     6061 cacctgctga gtggaaatcg taaggtacgg acgtttcgac atcgtgcgac aggccgaagt
     6121 gttcttcttc gacaataaac ccgccgccga cagagtaata ggttttactt aatagcgctt
     6181 cctgtccctt ccaggcagtg atccgcattc cgttctcatg gcgaggcaac atttcgggat
     6241 ggaagataat gttctttgct acaggaaaat caacaatatg cgcaccagat gccactggca
     6301 gccgcccgct gcgcgttact aactctataa atgcagggat ctcatcaatg acaacatcct
     6361 gcggactgtt tcctgccagt cccatgataa tggcgacatc cgtggcatgg cctttgcccg
     6421 tcagtgacaa cgacccgtac agatcgacca caatatggct cgtcgcggtt aataagccgc
     6481 tactttccag ccgatcaata aaactttttc cggcattcat tggccccacg gtatgcgaac
     6541 tggagggacc aatcccaatt ttgaaaatat cgaatgcact aatcatatcc acaccctcgg
     6601 attgccgttc agtgaagtgg agcggaacga ccttgcgacc gtcccgctca cgaggcttta
     6661 cgcactacgt actgcgatag cttcaatttc cagcttcaca tctttcggca aacgcgcaac
     6721 ctgcacacag ctccgggtcg ggtaggtcgc ctgatgctca tcgaagaact gcttatagac
     6781 ttcattgatg gtggcaaaat cattcagatc ggtgatgaac acggtcatct tgatgatatc
     6841 gcccacgctc agcccggcag caaccacgat cgctttgacg ttttcgaggc ttaaacgtgc
     6901 ctgatcttgc acatcagccg ggatctcacc ggtctgcggg caaaccggaa tttgcccgga
     6961 ggtgaagacc atgctgccta aatcaacgcc ctgaacataa gggccgattg cgcctggggc
     7021 acgttgcgtt tcgataatct ttttcataca tcctccggcg tcagagcgcc tgggtaaaag
     7081 tacgtgaaat aacatcctgt tgctgttcac gggtcagtgc gttgaagcgc acggcgtagc
     7141 cagagacacg gattgtcagg ttaggatatt tttccgggtg ctcgatggca tccagcagca
     7201 tttcccgatt cattacgttg acgttgaggt gttgaccgcc ttcgacatcc gcttcgtggt
     7261 ggaaataccc atccagcagg ccaacaaggt tggttttacg tactggatct tctttgccca
     7321 gcgccgcagg aacgattgag aaggtgtacg agatcccatc tttggcgtag gtgaacggca
     7381 gtttcgccac cgacgtcaat gaggccacgg cacctttgcg gtcacgacca tgcatcgggt
     7441 tagcgcccgg cgcgaacggt gttccggcgc gacgaccgtc cggcgtatta ccggttttct
     7501 ggccgtacac cacgttagaa gtgatagtca gaatcgactg ggtagggacg gcgttgcgat
     7561 aggttggcag cgctttaatt ttcttcataa agcgttcaac caggtcgcag gcaatgctgt
     7621 ctacgcgctc gtcgttgttg ccgtactgcg gatattcacc gtcgatttca aagtccaccg
     7681 ccaggccgtt ttcgtcacgg attggtttca cgcgggcata tttgatggca gacagggagt
     7741 ccgtcgccac cgacaggccc gcgatgccgc atgccatagt gcgatagaca tcacgatcgt
     7801 gcagcgccat cagcgaagct tcgtagctgt acttgtcgtg catgtagtga atgatattca
     7861 gcgcgctgat gtactgcacc gccagccagt ccatgaagtg atcgaggctg tccatcactt
     7921 tgtcgtaatc cagcacgtcg tccatcagcg gtgctgtttt cggcccgacc tgaatcttca
     7981 gcttctcgtc caccccgccg ttaattgcgt agagcagcgt tttcgccagg ttagcgcgtg
     8041 caccaaagaa ctgcatttgc ttaccaatca ccattgggct gacgcagcag gcaatcgcgt
     8101 aatcgtcgct gttgaagtca gtacgcatca gatcgtcatt ttcatactgc aaggaagagg
     8161 tgacgatcga cacctgcgcg gcatattttt tgaaggcaat cggtaattct tccgaccaaa
     8221 gaatggtcag gttaggttcc ggtgccggcc ccatagtgtg cagggtgtgc aaatagcgga
     8281 aggagttttt ggtcaccagc gtacgaccgt ccagccccat cccgccgatc acttccgtcg
     8341 cccagattgg gtcgccggag aacagcgaat caaattccgg tgtacgcagg aagcgtacca
     8401 tacggatctt catgatgaag tgatcgatca gttcctgtgc ctgctgctca ttgaggacgc
     8461 cagctttaaa gtcgcgctca atgtagatat ccaggaacga tgccgtgcgc cccagcgaca
     8521 tcgcgccgcc attttgcgat ttcactgccg ccagataagc gaagtagagc cactgcaccg
     8581 cttcctgtgc attctgcgcc gggcgagaga tatcaaagcc atatttcgcc gccatttcct
     8641 gaatctgcaa cagcgcatga cgatgctctg ccagctcctc acgcagacgg atggtggctt
     8701 ccagatcctc gcctttttcc agacgagact ggagatcggc aaattgcagt tcgcgttcac
     8761 gcaccagata actgatgcca tacagcgcta cgcggcgata gtcaccgata atgcgcccac
     8821 gaccatagcc atctggtaaa ccggtcagca cgccagattt acggcagcgc agcatatccg
     8881 gtgagtaaac atcaaatacg ccctggttat gggttttacg cagatcggta aacagatatt
     8941 caaattcact gtccatttca cggccatagg cgtggaatga acttttaatc atattaatgc
     9001 caccgaacgg gtgtagcgca cgtttcaacg gcgcatccgt ttgcaggcca acaatttttt
     9061 ccagcggctg gttaatatat cccgcatcat gagcggtaat tgtggtggca atattggtat
     9121 cgaaatcaac cggcgcgtgg gttgcgtttt cgatacggat gccttccatt accttttccc
     9181 acaattccgt ggtggcaggc gtcgcttcgg cgagaaaaga ttcatcgcct tcatacggtg
     9241 tatagttatg ttgaataaaa tcgcggacat taatttcgct tttccagtcc gtacctttaa
     9301 agccaagcca tgcgtcagcg tacagcttat cgctggtatc aatatctacc ttcatgaaaa
     9361 ataatctctc tacaatactt caactaaatt atgcaaattc tgcgggcgcg ttaactttac
     9421 ctaaatgaat ggcatccaaa gcaatcattt tttcttcgtt agtcggaata acgacgcaaa
     9481 tgacatgcgc attttcactg gaaacaattc gctcgccaaa agagttagat cgattattca
     9541 tttctgtatc aatctctacg cctaatacag ccaaatgttc catgaccaga cgacgaatta
     9601 aacttgaatt ctctcctatt ccgccggtga atataattcc atccagccga cgtaatgaag
     9661 ctgcatgtcc ggcaatatga cgggcaattc ggtgaacaaa ggttttaatt gccagttgcg
     9721 cgcgttcgtg accttcatgc caggcttttt ccagaacacg taaatccgaa gaaagaccgg
     9781 aaatacctaa taatcccgac tctttattca ctacgcgttc caggtcaccc aggctctggt
     9841 tggtttggct ggcgacccag gacatcgcac caaagtcgac atcgccactg cgggtaccca
     9901 tcatcaagcc ttccagcggc gtcattccca ttgaggtgtc aacactctga ccgttgcgaa
     9961 ctgcgcagat tgacgcgcca ttgccaagat gcgccacaac caggccggag tcatcttccg
    10021 ccagattcag cagcgaatgt gcgcgctggg aaacatagcg gtgcgacgtg ccgtggaaac
    10081 cataacggcg tacacctaac tcttcataat atttccacgg caggccgtat aaataagctt
    10141 ccggagccat cgtctggtgg aaactggtat caaataccgc cacctgagtt acgcccggaa
    10201 ataattgctg cgccgattca ataccactta aatttgcgta attatgcagg ggtgccagtg
    10261 gagaaacgcg acggatatta tcaatgactt catcggtaat aatggcggac tcggtaaaaa
    10321 tactgccgcc gtgagcgatg cggtggccaa ttaaggccac gctgtcattt aaattccgtt
    10381 tttccagttc aaatgcaatt gccttcaatg caccttcgta gctgtggtga gccagcggtg
    10441 ctggctctcc cccatttacg gataagaatg cattttccga gttaataccg tcggcaatac
    10501 ctgacattaa tacttcacaa tcgctggcat cgagtacgga aaacttaatc gaagacgaac
    10561 cacagttaat aaccaaaaca accggaaatt cattcatctc ttttctcatc ctgagttacg
    10621 gattaaaaca gtttgtatac gatgttcagg atggtcagca gaccaatcac ggtaacaaac
    10681 acgttatcca gacgaccacg gtatttcgcc agagacggcg ctttacggat agcatacatc
    10741 ggcaacaggc acagcaggga tgcgataatc ggtgcgccca tggcttcaat caggtcgagg
    10801 atgttcgggt tggcgtaggc aacaacccag gtggagccca tgatgaagat catgctgaga
    10861 gtattcagtt tacccagcga cactttggtt ttgtcacctt tataaccgaa cttcagaatc
    10921 agaccattca agccttccag cgtccccaga tagtgaccga agaaagattt gaagatagcc
    10981 acgagtgcga tgatggaagc cgcatattcc agtgtaatcg cgaacgttgt tttcgtaccg
    11041 gtcatggacg caaagtggtt agccagataa gaaagcactg gaatattctg cgctttggct
    11101 tccgccatgt tggccggaga cagagtaaac aggcagctaa aggcaaagaa catcaccact
    11161 gcaaccatca gcatgctggc acgagaaatg atttgggaac atttacgttc ggtgaagtcg
    11221 cgaccgaagt ctttctcata ctcttcacgt ttagaaacca cgaaggaaga gacgattggc
    11281 gagaagttaa aggagaaaac catgatggaa atccccagcc agacagtgat caggataccg
    11341 tcatgaccgg ttaacgacag cgaaccgagg tcaacctggt cgataactgc agagttccag
    11401 taagggatca gcgacagaga aatcagcacc aggctggcga taaacggcca taccaggtag
    11461 ctcatcactt taaccatcag atccttacca aaccagatga cgaaagccat cagcagcaac
    11521 aggaacagcg ccacaaagcc gcgattcagc ggtgcaaatc cgagctggtt ttcccagaac
    11581 gtcataaagg tattggtaat agtaacgcca taaatccaca gcagtgggca aatcgcgaag
    11641 aagtacagga acgtgataac cacgccgcca gttttaccaa aatgctcttc caccgtttcc
    11701 gtaatgttgc cggaagggtt agagccagaa agacacagac gcgccagcgc ccggtggcaa
    11761 taaaatgcga tggggtatgc caataccaac atcagaagaa tcgggatcag tccgccaaaa
    11821 cctgcgcgga tagggaagaa cagcaccccg gcgccgattg ccgtaccaaa caagcctaac
    11881 gtccatgtgg tatctgattt acgccaggac gattgttttg tctggctgga tacaatgcta
    11941 tctgaagtac tcatatccta tcctcaacga attaattaag cgtcaacgaa accggtgatt
    12001 tgagagacgc gagaaagatc gatattgccg ccggaaataa tactgacggt ttttctgttt
    12061 tgaatatatt ggtctaattt accgcttaat aatgcagcac atgccagagc gcctgcgcct
    12121 tcggtgacga ctttattgcg ctgaattaag gcaatcatac tgtttctgat ttcgtcttcg
    12181 ctgaccagca cgatgtcatc gactaattca cgaacgattt cgtaagttaa attacccggg
    12241 cgggagacat cacaaccatc cgccagggtg ccggtagttc ggtgcgtggt tatttctccg
    12301 gagtggaaag aagccgccat gccgtgaacg ttttctgact gtacgccaat aacacgaatg
    12361 gtcgggttaa tagatttaat tgccaccgca ataccagcaa ttaaaccacc accaccaatt
    12421 ggcacaatca cgttatcgac atcatagaga tcttccataa tttccagacc aatcgttccc
    12481 tggccagcaa tcactttcgg atcatcgtaa ggtgggataa aaatacgacc ttccatttcg
    12541 acaatttcgc tcactttagc gatagtgtcg ttgaagttat caccatgcag aacgacttct
    12601 gcggagtagt cgcacgttgc cgctactttg gattttggcg caccttttgg catcaccact
    12661 ttaccgtcga tacccagcat cgcgcaggag agcgaaaccc cttgcgcatg gttgcccgca
    12721 gaacaggcca ccacgccttt gcgtttttcc gcatcggtca gtgaacttaa tttattaaat
    12781 gcgccacgaa ttttaaatga acccgtacgc tgcatatttt caaacttcag gaatatttca
    12841 cctttgcaac gttcactaaa atagttggag cgaggcatgc ctgttttata aattcgccca
    12901 gccagtcgtt gtttcgcttc aataatatca tcaatagcaa ccggcagatc gtatgtaata
    12961 tgcattataa aacctcttcg cctgaattaa atatgtaggt aaccgtaacc gacacctgca
    13021 aaacagacag gtggattatt tatgtttaaa taaaaacaaa caactaacca acttctatta
    13081 attgccttcg tctacaccca ttataagatg aatactcttt ggctaattcc accaaaaccg
    13141 atgctgcttt tttaatacga taatttttcg accataccgc ggcatattgt gccacaggta
    13201 atgtttcttc aaccggaata gtaataaatt gattagaacc aaaaggtgac gtcatatcac
    13261 aaggaattac agttaagaaa tcagcattga gaacaagatt ataaattgtc acgactgagt
    13321 cggttttaac gatgttttca atactgatgc catttctttg taacgtagta agcagttcgc
    13381 tgtagtaccc catattagtt tgtggcaaca cccactgttc gttcttcaac gactccagcg
    13441 tggtggtgcc ggtgcatgtt cgggacttac tggctaccag cacaaactcg gactcgaaca
    13501 gcggctcaac atgtaaatcc tgaagcttca tttctgcact taacgtacca atcgcaaaat
    13561 ccagtcgacc gtcgcggatt gccggtaaga acgaagacag ttgcgcttca tacatagaaa
    13621 cctgcgcttt cgggaacacc tctttgaact tgttgatcat ccctgacata aaagtaaaac
    13681 caatcaatga aggaaaacca aatgagactt ccaccaccgc ttcagaagac ataccgctta
    13741 tctcattaac catatttttc atttcacggg taatggattc ggaacgggag agtaacaatt
    13801 gaccggcagg tgttaatgtt acaccggtat ttttccgcac cactaattcc acaccaaaat
    13861 aatcttcaat atcgttaatg attttactga cggccggttg agttaaccct aattcttttg
    13921 cagccgagcc gatagagcca cttctaatga cttcctgaaa gactaccagg tgctgcgttt
    13981 tcggaagaag aatagtgctc ataacgacct acgttaatta cctcattgac ggcatgaagt
    14041 gtatcaaaat gaaataaaca ggatatgtgc gaccactcac aaattaactt tcattacttt
    14101 ccaaggtatc gttattacaa ttaaatatct ttaaatatca acaagttaaa gcataaaaat
    14161 cagcataaac cctgattttt atcaaaaaaa aatgacgggg ataaccatat tttatggcga
    14221 taacatcatt cgttatgtgg ttaatacaaa aaaggggctg agaccatatt tcaagcaatt
    14281 accggataat taccaggcaa aatttgagtt aaatttaatg agcaagttct caaattttat
    14341 tataaataaa ccattttcag cgataaatac tgcggcacgt cacatttttt cacgctattt
    14401 gttggagaac aaacatttat tttatcaata ttttaaaatt tcgaatacag gtattgatca
    14461 tctcgaacaa ttgattaacg tcaacttttt ctcttctgac aggacgtcat tttgtgaatg
    14521 caatcgtttt ccataaattc ttctcccctc ataggcgacg aatagcattt tgtgttgagg
    14581 atcacaaaac gaataattgc tggtcgccgc gataaggtca gacgaagtca acaaggggga
    14641 ttttcactgt gctttacatc agcgtaggac acagaatgtt gcttcgttta ctgtaacgcc
    14701 gggtaaatga gcgttttttg atagtgcgta agtgtcgtgc gaggaaaata agcatctatt
    14761 attgtttgtc atgacgacaa ggcaggcatc aatgaaaggc ttcccaattg cacatatttt
    14821 tcacccttca atcccgccaa tgcacgcagt ggttaacaat cacaatagaa atattgatta
    14881 ttggacggta aaaagaaagt ttgcagaaat tgtctccacc aatgacgtta ataaaattta
    14941 cagtataagt aatgaactcc ggagagtatt atctgcaatt actgcattga atttctatca
    15001 aggcgatgtt ccttctgtca tgatccgaat ccaaccggaa aatatgagtc cattcatcat
    15061 agatatttct acaggagaac atgatgatta tatcatacaa acattagatg taggcacttt
    15121 tgcacctttt ggtgaacaat gtacttgctc agccgtcaat aaaaaagagc tggaatgtat
    15181 taaggagacg atttctaagt attgcgcaaa gttcacccaa aaagaagcca tcttaacacc
    15241 ccctaggcac tttaataaaa cctgcattac ttcagattgt tggcaaattc tctttttttc
    15301 acccgatcat tttaataatg atttttattg attatttata aaggataaaa taatgttccc
    15361 tgtttcatca attggtaatg atattagtag tgatttagtc cgtcgaaaaa tgaatgacct
    15421 tcctgaaagc cccataggaa ataacctcga agcactagca ccaggtatag aaaaactaaa
    15481 acagacctct attcaaatgg tcactttact taacgcgtta caaccaagtg gaaaatgcat
    15541 tatcactggt gattttcaaa aagaattagc gtacttacaa aatgtaattc tttataatga
    15601 ctcgtctctt cgtctggatt ttttaggtta taacacccaa attattcaac gatcggacaa
    15661 tacctgcgaa cttaccatta atgaaccgtt aaaaaactag gaaatatcta caggcaatat
    15721 caatgttaat tgcccattaa aagatattta caatgaaatc aggaggttga acgtaatctt
    15781 tagttgtggg actggagata tagttgatct atcctctctg gacttacgta atgtcgattt
    15841 agattattat gatttcacag ataaacatat ggctaatact attctaaatc attttaaatt
    15901 ggattcaaca aattttacta atgccaacat gtttcaggtt aattttgtta gttcaacaca
    15961 aaacgccaca atctcctggg attatttact aaaaataacg cctgttttaa taagcattag
    16021 cgatatgtat tctgaagaaa aaatcaagtt tgtcgaaagt tgtttaaatg agcttggaga
    16081 cattaccgaa gaacaattaa aaattatgag atttgcaatt ataaaatcta taccaagggc
    16141 aactcttaca gataaattag aaaatgaatt aacaaaagaa atatataaaa gctcatcgaa
    16201 aatcaacaat tgcttgaaca gaattaaatt accagagatg aaagaattct catcaaaaaa
    16261 aatatatgat tacatcgata caatcattga agattatgaa aatatcaaag aaaatgctta
    16321 tctggtcgtc ccccaaatta attatactat ggatttaaac atagaagact ctagctcaga
    16381 agagttaata ccctcgagaa agacgaaaat tctccggaca atggctttga ggtcggggaa
    16441 tataacacat atgaagcata taactcagag aatcaatatt ttaccagaga ggactatacg
    16501 tataattacg accttttaaa tgcaatatag acttaataac ttcttacaaa gtctattatt
    16561 aataccgtag atattcatct taccctgtgc accaaaagca aaaacttatc gacgcgggtt
    16621 tgaaactgat ggcaaccgca aaaatgcctg atgcgctacg cttatcaggc ctacgccatc
    16681 tctgcaatat attgaatttg cgtgcttttg taggcaggat aaggcgttca cgcagcatcc
    16741 ggcatgaata aagcgcactc aacaatctga aacccgccgg aacggtttat tacgtacatc
    16801 aggtgaaact gaccgataag ccgctttctt ttgggtatag tgtcgtggac agtcattcat
    16861 ctttctgccc ctccaaaagc aaaaacccgc cgaagcgggt ttttacgtat atcaggtgaa
    16921 actgaccgat aagccgcttt cttttgggta tagcgtcgtg gacagtcatt catctttctg
    16981 cccctccaaa agcaaaaacc cgccgaagcg ggtttttacg tacatcaggt gaaactgacc
    17041 gataagccgc tttcttttgg gtatagcgtc gtggacagtc attcatcttt ctgcccctcc
    17101 aaaagcaaaa acccgccgaa gcgggttttt acgtaaaaca ggtgaaactg accgataagc
    17161 cgggttctgt cgtggacagt cattcatcta ggccagcaat cgctcactgg ctcaagcagc
    17221 ctacccgggt tcagtacggg ccgtacctta tgaaccccta tttggccttg ctccgggtgg
    17281 agtttaccgt gccacggact gttaccagcc gcgcggtgcg ctcttaccgc accctttcac
    17341 ccttacctga tcccgcttgc acgggccatc ggcggtttgc tctctgttgc actggtcgtg
    17401 ggtttccccc ccaggcgtta cctggcaccc tgccctatgg agcccggact ttcctcccct
    17461 ccgcccgtct cccccgaaga ggacgacgac gaagcggcga ctgtctggtc agcttcggcg
    17521 cgcagtatag agggtttgcg cgcccttgtc accccgtgtt gcgcattcca atcgccagtg
    17581 tcgcggcaat attacgtgaa gcacggcaga tattgtcata agccccgcgg aatgcttcgt
    17641 ccaacgtacc tatgctggtc aatacgctga agaccgcatc aatgccatgc tgatgtacaa
    17701 cgccaacatc atcggtcagg ctacccgcaa tgccaatcac cggtttatgg tacttcttcg
    17761 ccacgtttgc gacgccaatc ggtaccttgc cgtgaatact ctggctgtca atacgccctt
    17821 caccggtgat caccagcgta caatcgtgaa tatgttcctc cagattcagc gccgtagtga
    17881 cgatttcaat accacttttc agttccgcac caagaaacgc catcagcacc gcacccatac
    17941 cgcccgcagc tcctgcgccg gggacatctt ttacatcaac atgcagcgct tttttaatga
    18001 catcggcata gtgagagagg ttattgtcca gctcaacaat catcgcttca ctggctccct
    18061 tttgtgggcc aaagatgcgc gatgcgccgt tatcgcccac cagcggattg gtgacatcac
    18121 aagcgacgcg aatgacgcaa tcttttaagc gcggatcgag gccggaaata tcaatatcat
    18181 tcagagtatt aagactaccg ccgccaaaac caatttcatt gccgttggcg tcgcataatt
    18241 tcgcccccag cgcctgtacc atgcctgcgc cgccatcatt tgtagcgcta ccgccaatgc
    18301 cgataataat gtttgtcgcg ccgctctcca gcgcctacag gattaactcg cctgtgccgc
    18361 gtgaagtggt cacgagtgga tcgcgttttt ccgcaggtac cagctccagc ccactggccg
    18421 ccgccatttc aataaacgcg gttttgccat cgccggagat cccccaactg gcattcactt
    18481 tctcgcccag cggccctgta acccaggcgt gacgttcaga accctgggtg gctgcaatca
    18541 tcgcttccac cgttccttcg ccaccgtcgg caaccggaat agaaacgtac tgtgcatcag
    18601 gaaaaatttc ccgaaatcct ttttctatcg cctgcgcaac ctcgctggca gataaacttt
    18661 ctttataaga gtctggggcg attacgattt tcatacctat gcctgttacc acatgacgcc
    18721 ggagggcgtt tctcttattc ggcctggatt ccaggcccgg attgcaatac gccatccggg
    18781 cacgacgtca ttaacgagta acttcgactt tcgccagttt ttcgtagtag cacgccaggg
    18841 cgctatgatc cgccgttcct aaaccatctg ctcgcagtgc ctgcatcatc tccataaccg
    18901 cagctgtgag cggcagttgc gcgccgacgc cgtgggaagt atccagcgca tttgccagat
    18961 ccttaatatg cagatcaata cggaagcccg gcttgaagtt gcggtccatc accatcggcg
    19021 ctttggcatc cagcacggta ctgcccgcca gtccaccgcg aattgcctga tacaccaggt
    19081 ccgggttaac gcccgcttta gttgccagcg ttaacgcttc tgacatcgcg gcaatattca
    19141 gcgccacaat gacctgattc gccagtttgg tgacgttacc tgcaccaatt gccccggtat
    19201 gcaccacgga acccgccatt gctttcatca aatcatagta tttgtcgaaa atagccttgt
    19261 cgccgcccac catcactgac agcgtaccgt cgatggcttt cggttcaccg ccgctcaccg
    19321 gagcatccag catatcaatg cctttcgctt tcagcgcttc gctgatttca cggcttgcca
    19381 gcggtgcgat agaactcata tcgatcaata ccgtacctgg cttcgcgcct tcaataatgc
    19441 cattctcacc cagcgccacc tctttcacat gaggggagtt tggcagcatg gttatgatga
    19501 cgtcgcactg ttcagcgatc gctttagccg tagaggctgt ttctgcacct gcagcaatca
    19561 cgtcagcaat agcttctggg ttacggtcag caaccaccag cgagtaacct gctttcagaa
    19621 ggtttttact cattggttta cccataatcc ccaggccaat aaaaccaact ttcatagtca
    19681 tatcaatcat ctctcttgtt gcggtggtgg ttatttttta aaggtatcag ccagtttatg
    19741 agtggcagag cggaagacgc cgagatcgct gccgacagcc acaaacgtcg cgccccattc
    19801 cagataacga cgcgcatcgg cttcgaccgg cgcgaggata ccgctgggtt tgccgtgcgc
    19861 gctggcacgg ttaaaaatgt gctgaattgc tttttgtaca tccgggtgtg atgcattgcc
    19921 gagatggcct aatgccgcgg ccagatcgct ggggccgacg aagatgccgt ctacgccttc
    19981 ggtagcggca atggcatcga cgttatctac gccctgctga ctttctatct ggaccagaat
    20041 agtgatgttc ttgttcgact gagcgaaata atccgccacg gtgccaaaca tattggcgcg
    20101 gtgagaaacg gagacgccgc gaatgccttc cggtgggtaa cgggttgatg ccaccgcctg
    20161 ctctgcttcc tcttttgttt ctacaaaagg aatcaggaag ttatagaaac cgatatccag
    20221 aagacgctta ataatttccg gctcgttggt cggcactcac actactggcg cgctggcgct
    20281 gcctttcaag gccattaact gcggaataaa cgtggagata tcgtttggcg catgttcgcc
    20341 atccagcacc agccagtcaa acccagccaa accaagaact tcagtgctaa tcgggttaga
    20401 gagtgctgac cagcaaccaa tttgtacctg tttcgcagcc agtgcggctt tgaatttatt
    20461 cgggaaaaca tcgttattca tcgcttatac ctttgcttat ttctgcaatt ccatacgttt
    20521 aatgtcgcca actacgaaga ggtagcagac catcgccatc agcgctgaac atcccacgaa
    20581 aaccagtgct gcattgaagg agtgcagttc acttaccagg tagccaatca ccagtggagt
    20641 gacaatggag gcaacattgc caaagacgtt aaagacgccg ccacagaggc caacaatctc
    20701 tttcggcgcg gtgtcagaaa tcaccggcca gcccagagca ccaaatcctt tgccaaaaaa
    20761 agccagcgcc atcagcatga ccaccagcgt ggtgttgttg gtgtagttac ataagatgat
    20821 ggtggaagcc agcaacattc ccagcacaat cggtagctta cgtgccaggg tcagggataa
    20881 accgcgtttg atcagataat ccgagaagac acctcccagc acgccgcccg caaaaccaca
    20941 cagtgccgga atcgaggcga ccagacccac tttcagaatc gacatgcctt tttcctgcac
    21001 cagataaatc gggaaccagg tgaggaagaa ccaggtgatg gtgttgataa aatattgtcc
    21061 gaaaaatacg cccaacatca tgcggttaga gagcaattgc ttgatgtaat gcagtttgag
    21121 accgcttgct gccgcgctgc ccggcttttt gtggtccata tcgaccaccg cgccattttc
    21181 agagataaac ttcagctctt cctcagacat acgtgggtga tctgtcgggt tatgaatcaa
    21241 cttgatccac agcgccgtca gcacaaaaca aatcaccccc ataacggtaa agacgtgctc
    21301 ccatccccag gcgaaagtca gccagccaag cagaggcgaa aagagcgcca gcgagaaata
    21361 ttgtgccgag ttaaagatgg cggaggcagt accacgttct ttcgtcggga accaggcggc
    21421 gacaatacgg gcgttcgccg ggaatgatgg agcttccgag aatcccagca taaagcgcat
    21481 aaagaacatg gagatccctg cccaggccag cgggaacata tcaacaaagc cttgcaggaa
    21541 ggtgaacagc gaccagaaaa agaggctgta ggtgtaaact tttttcgagc caaacttatc
    21601 aagcaaccag ccgccgggga tttgcatcag caagtaggcc cagccaaaag cggagaagat
    21661 gtaacccatc gaaaccgcac ttaactgcaa ctcttttgcc acttcggtac cagcaataga
    21721 cagcgttgca cgatcggcgt agttaacggc ggtaacaata aaaataatca gtaatattaa
    21781 atagcgggta tgcacacctt tccttttttc gtcaacggtg tccagaatca ttttatttac
    21841 ctcgggtact tatgctgatt tttattatta tggggaaggt gttatttata agtttcattt
    21901 atgccgtaac gacaatgaac tcgggaatta gtataagcag cgcgagaata ataatcattg
    21961 tgcaaatgct aatttaatta atacttttta aatattattt tgagcatatg cacataaggt
    22021 tgcgcgctaa agcacagatt tgcgctttac cttgccgggc ggcactgcaa tccctgaaat
    22081 gattgacatt gatcacattt ctgcgtttaa actcctgaca ttcttatttc acccaatgaa
    22141 atcatttatt tttaaatgag accaggtcct cattttaata acccctggct ggagaatatt
    22201 gcacaatggc caacatcgaa atcagacaag aaacgccaac tgcgttttat ataaaagttc
    22261 acgacacaga taatgtggca attattgtta atgataatgg cctgaaagca ggaacgcgtt
    22321 ttccggatgg gctggaatta attgaacata ttccccaggg gcataaagtc gcattgctgg
    22381 acattccggc taatggtgaa attattcgtt atggcgaagt gattggttac gccgtgcgtg
    22441 caatcccacg cggaagctgg atcgacgaat caatggttgt actaccggaa gcgccgccgt
    22501 tacacacgct gccactggca accaaagtcc cggaaccctt accgccgctg gaaggataca
    22561 cctttgaggg ctatcgcaat gccgatggca gcgtgggcac caaaaacctg ctcggcatca
    22621 ccaccagcgt ccactgtgtg gcaggcgtgg tggactatgt agtaaaaatc attgaacgcg
    22681 atctgctacc gaaatacccg aacgtcgatg gcgtggtggg gctgaatcat ttgtacggtt
    22741 gtggcgtggc gattaacgca ccggcagcag ttgtacctat tcgtaccatt cacaatattt
    22801 cgctgaaccc taactttggc ggcgaagtaa tggtgattgg cctgggttgt gaaaagttgc
    22861 agcctgagcg cctgctgact ggaacggatg atgtgcaagc tattccagta gaaatcgcca
    22921 gcattgtcag tttgcaggat gaaaagcatg tcggttttca gtccatggtc gaggatattt
    22981 tgcaggtcgc cgaacgccat ctacaaaaac tgaatcaacg tcagcgagaa acctgtccgg
    23041 cttcagaact ggttgtcggc atgcattgcg gtggcagcga tgcgttttct ggcgtaacgg
    23101 caaacccggc agttggctat gcgtctgatc tactggtgcg ctgcggcgca acggtgatgt
    23161 tttcagaagt cacggaagtg cgtgacgcga tccatctgct gacaccacgc gcagtgaacg
    23221 aagaggtcgg caaacggctg ctggaggaga tggagtggta cgataactat ctcaatatgg
    23281 gaaaaaccga ccgcagcgcc aacccttcgc cgggcaacaa gaaaggcggt ctggcaaacg
    23341 tggtagagaa agcgctcggc tccattgcta aatcgggcaa aagtgcaatt gttgaagtgc
    23401 tgtcgcccgg tcaacgcccg actaaacgcg gattaattta cgccgcgacg ccagccagcg
    23461 attttgtctg tggcacgcaa caggtggctt cgggtatcac cgtgcaagtg tttacgaccg
    23521 gtcgtggtac gccgtacggc ctgatggcgg tacccgtcat taaaatggca acccgcaccg
    23581 agctggcgaa ccgctggttt gatttaatgg atattaatgc gggcaccatc gctaccggcg
    23641 aagaaactat tgaagaggtg ggctggaagt tgttccactt tattctcgac gtcgccagcg
    23701 ggaagaagaa aaccttctcg gatcaatggg gactgcataa ccagctggcg gtgtttaacc
    23761 cagcaccggt gacctgataa ttcaattcgt attatgcccg atagcacacg tttatcgggc
    23821 atattgagtc acagcgaaga cgcgtgatcc ccgaccagaa tcacttcaac cccagccttt
    23881 cgcagtcctt ccagactatc cgcaggaatg ccttcatcaa caatgatcat gtcgatacgt
    23941 tgagtatcaa tgatcttatg taaactggaa cgattgaatt tactggaatc ggtgaccacg
    24001 atgatccgtt ccgcaacttc gcacatccga cggtttaaac gggcttcatc ttcattatgt
    24061 gtgctgacgc cgcgctccag atcgatcgca tctacaccaa gaaacagcat atcgaagtgg
    24121 taattttgca gcgattgctc agcctgatcg ccgtaaaaag attgcgactg acggcgcaaa
    24181 tgcccgccgg tcatcagcag ctcaacgcct tccgcttcca gcaacgcatt agccacgttc
    24241 ataccgttgg tcatcgcaat tacgtcagtg tacttgcgca tcagacgagc aatctcaaaa
    24301 gtggtggtcc cggaatcgag gatcacccga tgacctggct gaatcaactc aacggcagct
    24361 ttcgcaacgc tgcgtttcat cgcggtgttc agtgcgcttt tatcttccac tgatggctcg
    24421 actgacggcg tcgtgctatc gcagatcaac gcgccaccat aggcacgcac agcgatcccc
    24481 tgcttttcca gaaacgccag atcattgcgg atcgtcacag tagatacgcc atacaatgcc
    24541 gacagatcgt taacctgcac actcccttgc tgtcgcagac gctgaatgat ctgttctcgt
    24601 cgctcgctgg tgcctgtcac tcgcttctca cctgaagcgt cggtattact catagtaagt
    24661 cctttcgtaa aactttcgtt tcatttcgtt ttgcctatta acgcctttct attaagcaaa
    24721 tgcaagccca ccttgcccat tggcgcaagc tactctcgtt tcagtgactt tcattacgtt
    24781 tcttttgtga atcagatcag aaaactatta tctttcgttt tatttttatc tcaccatgac
    24841 gcagtatcaa ttgaaacaaa acgaaagatc aatatcgcag taatctgaac tggagaggaa
    24901 agtgaaacat ctgacagaaa tggtgagaca gcacaaagcg ggcaaaacaa atgcaattta
    24961 tgccgtttgt tccgcacatc cgctggtgct ggaggctgca atccgctacg ccagtgcaaa
    25021 ccaaacgccg ttactgattg aagcaacctc caatcaggta gaccagttcg gcggttatac
    25081 cggaatgacg cccgccgatt ttcgcggctt tgtttgtcag ctcgccgact cgttgaattt
    25141 cccgcaggat gcgctgattc tgggtggtga ccatctgggg ccaaaccgct ggcaaaacct
    25201 gccagccgct caggcaatgg ccaatgccga tgatttgatt aaaagctacg ttgcggcagg
    25261 attcaaaaaa atccaccttg attgcagcat gtcctgtcag gacgatccaa ttcccttaac
    25321 tgatgacatc gtggctgaac gcgccgcccg tctggcgaaa gtggcggaag aaacctgtcg
    25381 cgaacacttt ggcgaagccg atctggagta tgtcattggt accgaagtgc cggtacctgg
    25441 cggcgcgcat gaaaccttaa gcgagctggc ggtcaccacg ccggatgccg cccgcgccac
    25501 gctggaagcc catcgtcacg cctttgaaaa gcaaggtttg aatgccatct ggccacgcat
    25561 tattgccctg gtggttcaac ccggcgtcga attcgatcac accaacgtta ttgattatca
    25621 gcccgccaaa gcgagcgcct taagccagat ggtcgaaaac tacgaaacgc tgattttcga
    25681 agcgcactct accgattatc aaacgccgca atctctgcgc cagctggtga ttgaccactt
    25741 tgccattctg aaagttggcc cggcgctgac cttcgccctg cgtgaagccc ttttttctct
    25801 ggcggcgatt gaagaagaac tggtaccagc gaaagcctgt tccggtctgc gtcaggtgct
    25861 ggaaaacgtg atgctcgacc gcccggaata ctggcaaagc cactaccacg gtgacggcaa
    25921 cgcgcgtcgt ctggcgcgtg gttacagcta ctcagatcgc gtgcgctatt actggccgga
    25981 cagccagatt gatgacgctt tcgctcatct ggttcgtaat ctggcggatt caccaattcc
    26041 gctaccgctc atcagccagt atctgccgct gcagtacgtg aaagttcgct ccggcgagct
    26101 gcaaccgacg ccacgggaac tcattataaa ccatattcag gacatcctgg cgcagtacca
    26161 cacagcctgt gaaggccaat aagcaaaaca aagaggaaca tgctatgcca aatattgttt
    26221 taagccggat tgatgaacgc ttgattcacg gtcaggtcgg cgttcagtgg gtcggatttg
    26281 cgggggcaaa tctggtgctg gtagccaacg atgaggttgc cgaagatccg gtacaacaaa
    26341 acctgatgga aatggtactg gcagaaggga tcgccgtacg tttctggacg ctgcaaaaag
    26401 ttatcgacaa cattcatcgc gccgccgatc gacagaaaat cctgctggtt tgtaaaacac
    26461 ccgccgattt cctgacgctg gtgaaaggtg gcgttccggt gaatcgcatt aacgttggca
    26521 atatgcacta cgccaatggc aaacaacaaa tcgccaaaac ggtttctgtg gatgcgggcg
    26581 atatcgcagc atttaacgac ctgaaagccg ctggggtgga atgcttcgtt cagggcgtcc
    26641 cgacagagcc tgctgtggac ctctttaaat tactttgagg gattcatcat ggaaatcagc
    26701 ctgttgcagg catttgcgtt gggcattatc gcctttatcg ctggcctaga tatgtttaac
    26761 ggcttaaccc atatgcaccg cccggtggtc ctcggcccgt tggtcgggct ggtacttggc
    26821 gatctgcata ccggaatttt aaccggcgat acgctggaac tagtgtggat ggggctggcc
    26881 ccgctggcgg gcgcacagcc gcctaacgtg attatcggta ctatcgtcgg cacggcgttt
    26941 gccattacta ctggcgtgaa acccgatgtc gcggtcggtg tcgccgtacc tttcgctgtc
    27001 gcagtacaga tggggattac cttcctgttc tcggtgatgt ctggcgtgat gtctcgctgc
    27061 gaccggatgg cggagaacgc cgatactcgc ggcattgaac gcgtgaacta tctggcactg
    27121 ctggcactcg gcactttcta ttttctctgc gctttcctgc ctatctactt cggcgcggaa
    27181 catgcaaaaa ccatcattga tgtcctgccg caacgattaa tcgacggcct cggcgtcgcg
    27241 ggcggcatca tgccagcaat cggttttgcc gtgctgctga aaatcatgat gaaaaacgtc
    27301 tacataccct acttcatcct gggctttgtt gccgcaacct ggctcaagtt accggtgctg
    27361 gctattgctg ccgccgcgct ggcgatggcg ctgatcgatc tgctgcgtaa atctcctgaa
    27421 ccgactcaac ctgcggcaca gaaagaggaa tttgaagatg gcatctaatc aaaccaccct
    27481 gccgaacgtc agcgaaaatg aagaaacact gctgactggc gtcaatgaaa acgtgtatga
    27541 agatcggagc attgccgcgg agctgacgaa aaaagatatc aaccgcgtcg cctggcgttc
    27601 catgctgtta caagcgtctt ttaactacga acgtatgcag gcttctggct ggttgtacgg
    27661 tctgctgcct gctctgaaaa agatccacac taataaacgc gacctggcgc gcgccatgaa
    27721 ggggcatatg ggtttcttca atacccatcc gtttctggtg acgtttgtta tcggcattat
    27781 ccttgcgatg gagcgttcta agcaggacgt taacagtatt cagagcacca aaattgccgt
    27841 cggtgcgccg cttggcggga ttggcgatgc gatgttctgg ctaacgctac tgccgatttg
    27901 tggcgggata ggcgcaagcc tcgcgctaca aggctctatt cttggcgcag tcgtctttat
    27961 tgtgctgttc aacgtggtac acttggggct gcgttttggt ctggcgcatt atgcttaccg
    28021 catgggcgtg gcggcgattc cccttattaa agcgaatacc aaaaaagtcg gccatgctgc
    28081 gtcaatcgtt ggaatgacgg taatcggcgc actggtggca acctatgttc gtttaagcac
    28141 cacgctggaa atcacggcgg gcgacgcagt agttaagtta caggctgatg ttatcgacaa
    28201 actgatgcct gccttcttac cgctggtcta caccctgacc atgttctggc tggtacgccg
    28261 cggctagagt ccgctgcgcc tgattgcggt taccgtggtt ctagggatcg tcggtaaatt
    28321 ctgccatttc ctttaaatac aatgaggtat tagatgttaa gtattatttt gacagggcat
    28381 ggcgggtttg ccagtggcat ggaaaaagca atgaagcaaa tcctcggcga tcaatcgcag
    28441 tttattgcca tcgattttcc cgaaacctca agcgccgcgc tgcttacatc gcagcttgaa
    28501 gaggccatcg cccaactgga ttgtgaagac ggcattgttt ttctgacgga tttgctgggc
    28561 ggcacgccgt ttcgcgtagc ttcaacgctg gcgatgcaaa aaccgggctg tgaagtgatt
    28621 accggcacca atttgcaact gctactggag atggtgctgg agcgcgaagg gttaagcggt
    28681 gaagaattcc gtgtacaggc gctggagtgt ggacaccgag ggctgaccag tctggtggac
    28741 gagttaggcc gctgccatga agaatgtccg gtcgaggaag gaatatgaca cacgttctgc
    28801 gcgccagaag gctgctgact gaagagggat ggttcgatga ccatcagttg cgtgttgctg
    28861 acggtgtcat cgcagcaatc gaaccgattc cagtgggcgt gactgaacgc gatgcggaac
    28921 tgctctgccc cgcttacatc gacacccatg tacacggtgg tgcgggcgtt gatgtaatgg
    28981 atgacgcgcc ggatgtactc gacaagctgg caatgcacaa ggcacgcgaa ggtgtcggca
    29041 gttggttacc gaccaccgta accgcgccgc ttaataccat tcatgcggcg ctgaaacgta
    29101 ttgctcaacg ttgccaacgc ggcggacctg gtgcgcaagt gctggggagt tatctcgaag
    29161 gaccgtactt cacgccgcag aataaaggcg cgcatcc
//
LOCUS       DAEEUE010000045        29051 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.045, whole genome shotgun sequence.
ACCESSION   DAEEUE010000045 DAEEUE010000000
VERSION     DAEEUE010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29051
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.045"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            109..1140
                     /gene="mdtN"
                     /locus_tag="KLK84_002138"
     CDS             109..1140
                     /gene="mdtN"
                     /locus_tag="KLK84_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HBE2009454.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWLVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1140..3188
                     /gene="mdtO"
                     /locus_tag="KLK84_002139"
     CDS             1140..3188
                     /gene="mdtO"
                     /locus_tag="KLK84_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HBE2009455.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYG
                     EPLIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIV
                     VGLYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALA
                     LQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLA
                     SEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPAA
                     KPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGS
                     SYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLAAWIATSSERSS
                     YIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTL
                     PQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDS
                     EERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLA
                     TALSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ
                     "
     gene            3185..4651
                     /gene="mdtP"
                     /locus_tag="KLK84_002140"
     CDS             3185..4651
                     /gene="mdtP"
                     /locus_tag="KLK84_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HBE2009456.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4849..5088
                     /locus_tag="KLK84_002141"
     CDS             4849..5088
                     /locus_tag="KLK84_002141"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009457.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            5098..5268
                     /locus_tag="KLK84_002142"
     CDS             5098..5268
                     /locus_tag="KLK84_002142"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBE2009458.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5317..6729
                     /gene="fdhF"
                     /locus_tag="KLK84_002143"
     CDS             5317..6729
                     /gene="fdhF"
                     /locus_tag="KLK84_002143"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HBE2009459.1"
                     /translation="MSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
                     CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKI
                     VESYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLA
                     MLTSNLGKPHAGVNPVRGQNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVES
                     LPAHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFI
                     TKTASAADVILPSTSWGEHEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMG
                     YPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKF
                     DTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPG
                     YAQINTEDAKRLGIEDEALV"
     gene            6730..6996
                     /locus_tag="KLK84_002144"
     CDS             6730..6996
                     /locus_tag="KLK84_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HBE2009460.1"
                     /translation="MHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTESLSP
                     ITKTPEYKYCAVRVEPISDQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7090..7779
                     /locus_tag="KLK84_002145"
     CDS             7090..7779
                     /locus_tag="KLK84_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBE2009461.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7857..9170)
                     /gene="gltP"
                     /locus_tag="KLK84_002146"
     CDS             complement(7857..9170)
                     /gene="gltP"
                     /locus_tag="KLK84_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HBE2009462.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9512..10108)
                     /gene="nrfG"
                     /locus_tag="KLK84_002147"
     CDS             complement(9512..10108)
                     /gene="nrfG"
                     /locus_tag="KLK84_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HBE2009463.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10105..10488)
                     /gene="nrfF"
                     /locus_tag="KLK84_002148"
     CDS             complement(10105..10488)
                     /gene="nrfF"
                     /locus_tag="KLK84_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HBE2009464.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10481..12199)
                     /locus_tag="KLK84_002149"
     CDS             complement(10481..12199)
                     /locus_tag="KLK84_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HBE2009465.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLCLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12219..13175)
                     /gene="nrfD"
                     /locus_tag="KLK84_002150"
     CDS             complement(12219..13175)
                     /gene="nrfD"
                     /locus_tag="KLK84_002150"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HBE2009466.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13172..13843)
                     /gene="nrfC"
                     /locus_tag="KLK84_002151"
     CDS             complement(13172..13843)
                     /gene="nrfC"
                     /locus_tag="KLK84_002151"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HBE2009467.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHVPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13840..14406)
                     /gene="nrfB"
                     /locus_tag="KLK84_002152"
     CDS             complement(13840..14406)
                     /gene="nrfB"
                     /locus_tag="KLK84_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HBE2009468.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14451..15887)
                     /gene="nrfA"
                     /locus_tag="KLK84_002153"
     CDS             complement(14451..15887)
                     /gene="nrfA"
                     /locus_tag="KLK84_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HBE2009469.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16280..18238
                     /gene="acs"
                     /locus_tag="KLK84_002154"
     CDS             16280..18238
                     /gene="acs"
                     /locus_tag="KLK84_002154"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HBE2009470.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAMHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            18438..18752
                     /locus_tag="KLK84_002155"
     CDS             18438..18752
                     /locus_tag="KLK84_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HBE2009471.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18749..20398
                     /gene="actP"
                     /locus_tag="KLK84_002156"
     CDS             18749..20398
                     /gene="actP"
                     /locus_tag="KLK84_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HBE2009472.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20576..21868
                     /locus_tag="KLK84_002157"
     CDS             20576..21868
                     /locus_tag="KLK84_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBE2009473.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22022..23671)
                     /locus_tag="KLK84_002158"
     CDS             complement(22022..23671)
                     /locus_tag="KLK84_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HBE2009474.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23822..25171)
                     /gene="ghxP"
                     /locus_tag="KLK84_002159"
                     /gene_synonym="yjcD"
     CDS             complement(23822..25171)
                     /gene="ghxP"
                     /locus_tag="KLK84_002159"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HBE2009475.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25877..26341)
                     /gene="soxR"
                     /locus_tag="KLK84_002160"
     CDS             complement(25877..26341)
                     /gene="soxR"
                     /locus_tag="KLK84_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HBE2009476.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26427..26750
                     /gene="soxS"
                     /locus_tag="KLK84_002161"
     CDS             26427..26750
                     /gene="soxS"
                     /locus_tag="KLK84_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HBE2009477.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26753..28330)
                     /gene="pdeC"
                     /locus_tag="KLK84_002162"
                     /pseudo
     CDS             complement(26753..28330)
                     /gene="pdeC"
                     /locus_tag="KLK84_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            28759..29040
                     /locus_tag="KLK84_002163"
     CDS             28759..29040
                     /locus_tag="KLK84_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HBE2009478.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         7289 a         7779 c         7349 g         6634 t
ORIGIN      
        1 cgggcaaata tcaatctggc atttgtcgga attatttata ccgccctttt tgctctctac
       61 gccatgctgt tctggctggc atttttctaa tctatttgag acgcgctcat ggaaagtacg
      121 ccgaaaaaag cacctcgcag taaattccct gctctgttag tggttgcgtt ggcgctggtt
      181 gcccttgttt tcgttatctg gctcgtagac agtgcgccat caactaatga cgcttacgcg
      241 tcagcagata ccattgatgt ggtgccggaa gtcagcggtc gcattgtaga actggcggtc
      301 accgacaacc aggcggtgaa acagggcgat ttgctgttcc gcatcgaccc gcgcccgtac
      361 gaagccaacc tggcgaaagc cgaagcctcc ctcgcggcgc tggataagca aattatgctc
      421 acccagcgta gcgttgacgc ccaacagttt ggtgccgact cggttaatgc cacggtagaa
      481 aaagcccgtg ccgccgcgaa acaggccaca gatacattac gccgcaccga gccattactg
      541 aaagagggtt ttgtctcagc agaagacgtt gaccgtgcaa gaacggcgca gcgcgccgca
      601 gaagcggatc ttaatgccgt attgttacag gcgcagtcag ccgccagcgc cgtcagcggc
      661 gtcgatgcgc tggtggcgca acgtgcggcg gtcgaagcgg atattgccct gaccaaacta
      721 catctggaaa tgacgaccgt tcgcgcgcca tttgatggcc ggatcatttc cctcaaaacc
      781 tccgtcgggc aatttgcttc tgccatgcgc cctattttta ccctcatcga cactcgtcac
      841 tggtacgtga tcgccaactt ccgcgaaacc gatctgaaaa atattcgctc aggtacaccc
      901 gcaacgattc gcctgatgag tgacagcggc aaaaccttcg agggtaaagt ggattcgatt
      961 ggctacggcg tgctaccgga tgacggcggc ctggtgctgg gcggcctgcc aaaagtgtct
     1021 cgttctatta actgggtccg cgttgcccag cgttttccgg tcaaaatcat ggtcgataaa
     1081 cctgacccgg aaatgttccg catcggcgct tcggcagtcg ctaatcttga gccgcaataa
     1141 tgagcgcgct caactccctg ccattaccgg tggtcaggct gctggcgttc tttcatgaag
     1201 agttaagcga gcggcgaccc ggccgcgtgc cgcagatcgt gcaactctgg gtaggctgcc
     1261 tgctggtgat tctgatctcg atgacttttg agatcccttt tgtggcgtta tcgctggcag
     1321 tgctgtttta cggtatcggt attcagtcga acgcgtttta caccaaattt gtggcgatct
     1381 tgtttgtggt tgccacggtg ctggagatcg gcagcctgtt tttgatctac aaatggtcat
     1441 acggcgaacc gttgatccga ttgatcatcg ccgggccgat cctgatgggc tgcatgtttt
     1501 tgatgcgcac ccatcggctg ggactggtct ttttcgctgt cgccatttac gggcaaacct
     1561 tccccgccat gctcgactat ccggaagtag tcgtgcgctt aacgctgtgg tgtatcgttg
     1621 ttggcctcta tccaaccttg ctgatgacgt taatcggcgt gctgtggttt cccaatcgtg
     1681 ccattacgca aatgcatcaa gcgcttaatg atcggcttga tgatgccatt agccacctga
     1741 cggacagcct cgcaccgcta cccgaaacgc ggattgaaag agaggcgctg gcgctgcaaa
     1801 aactgaatgt cttttgtctc gcggacgatg ccaactggcg aactcaaagc gcatggtggc
     1861 aaagctgcgt ggcaacggta acctacattt actcgacgct gaatcgctac gatcccacct
     1921 cttttgctga ttctcaggca attattgaat ttcggcaaaa attagcttca gaaatcaaca
     1981 agctgcagca tgccgttgct gaaggtcagt gctggcaaag cgactggcgg atcagtgaaa
     2041 gtgaagcggt ggcggcacgg gaatgtaacc tggagaatat ctgccagacg ttgttacaac
     2101 tgggccagat gaacccgaat acgccgccaa cgcccgccgc caaaccgcca tcaatggtcg
     2161 ccgatgcttt taccaatcca gactatatac gctacgcggt aaaaacattg ctcgcctgtt
     2221 tgatctgtta caccttttac agcggcgtgg actgggaagg cattcacacc tgtatgctga
     2281 cctgcgtgat cgtcgctaat ccaaatgtcg gttcgtcgta ccagaagatg gtgctgcgtt
     2341 ttggcggggc cttttgcggc gcgattctgg cgctgttatt cacgctactg gtcatgccct
     2401 ggctggacaa tattgtcgaa ttgctgtttg tgctggcacc gattttcctg ttggccgcat
     2461 ggattgccac cagctctgaa cgctcttctt atatcggcac acagatggtg gtcaccttcg
     2521 cgctcgccac gctcgaaaac gtttttggcc cagtgtacga cctggtggaa attcgcgatc
     2581 gcgccctggg tatcatcatt ggtaccgtgg tgtccgcgat gatttacacc tttgtctggc
     2641 ctgaaagtga agcgcgcacg ctgccgcaaa aactggctgg cgcgctgggt atgttaagta
     2701 aagtaatgcg gatcccacgc cagcaggaag tcacggctct gcgcacttat ctgcaaattc
     2761 gtatcggtct gcatgcggcg tttaatgcct gtgaagagat gtgccaacgc gtggcgctgg
     2821 agcgtcaact ggacagcgaa gaacgcgcct tactgattga acgttcgcaa acggttattc
     2881 gtcagggccg cgatatcctt cacgcctggg atgccacctg gaattcggcg caggcgctgg
     2941 ataacgcact acagccggac agagcaggtc agtttgccga cgccctggag aaatacgctg
     3001 ccggtctggc aaccgcactc agccgttctc ctcaaataac gcttgaagag acacccacct
     3061 cgcaggccat cctgcccacc ttattaaaac aggagcaaca cgtctgccag cttttcgccc
     3121 gcttgccaga ctggacagcc ccggcattaa cgcccgccac ggaacaggca caaggagcca
     3181 cgcaatgatc aatcgtcaac tttcacgtct gctgttgtgc agcattctcg gcagcacgac
     3241 gctgatttcc ggctgtgccc tggtacgtaa ggattctgcg cctcatcaac agctcaaacc
     3301 ggaacaaatc aaactggccg atgatattca tcttgccagc tccggctggc cgcaggcgca
     3361 gtggtggaaa caactcaatg acccgcagtt ggatgccctg atccaacgga cgctaagtgg
     3421 ttcacacacc ctcgccgaag cgaaactgcg ggaagaaaaa gcgcaatcgc aggccgattt
     3481 gttagatgcc ggttcacagt tgcaggtggc agcgttaggg atgcttaacc gccaacgtgt
     3541 ctcggcgaac ggctttttaa gcccttatgc gatggatgcg cccgcactgg ggatggacgg
     3601 gccgtactat acggaagcca cagtaggttt gtttgccgga ctggatcttg atttgtgggg
     3661 tgtgcatcgc tcagcggttg ccgccgccat tggcgcgcat aatgccgcgc tggcagaaac
     3721 cgcagcagta gagctatcgc tgaccacggg cgtagcgcag ctttattaca gtatgcaggc
     3781 cagctatcag atgctcgatc tgttagaaca aactcgcgat gtgattgatt acgcggtgaa
     3841 agcgcaccaa agtaaagtgg cgcacggtct ggaagcacaa gtgcctttcc acggcgcgcg
     3901 ggcacagatt ctggcggtcg ataaacaaat tgctgccgtc aaagggcaaa tcaccgaaac
     3961 gagagaatct ctgcgcgcat tgattggcgc gggagccagc gatatgccgg agatcaaacc
     4021 tgtggcatta ccacgagtcc agaccggcat tccggcgaca ctctcttatg agttgctcgc
     4081 cagacgcccg gatctgcaag ccatgcgctg gtatgttcag gcgtcattag atcaggtgga
     4141 ttccgcgcgg gcgttgttct atccgagctt tgatatcaaa gcgtttttcg gccttgatgc
     4201 catccacctg gataccttat tcaaaaaaac cagtcgccag ttcaacttca tcccgggtct
     4261 gaaattgccg ctgtttgacg gtggacggtt gaatgccaat ctcgaaggca cgcgcgccgc
     4321 cagcaacatg atgattgaac gttacaacca gtcagtactg aacgcggtgc gtgacgttgc
     4381 cgtcaacggc acgcgtctgc aaacgctcaa cgacgagcga gaaatgcagg ctgaacacgt
     4441 ggaagcaacg cgcttcaccc agcgcgctgc cgaggccgcc tatcagcgcg gcttaaccag
     4501 ccgcttacag gccactgaag cccggttgcc agtacttgcc gaagagatgt cattactgat
     4561 gctggacagc cgccgggtga tccaaagcat tcagttgatg aaatcgctgg gcggcgggta
     4621 tcaggcggct cccgtcgtcg agaaaaaata aaatgtctgc cgcgtgatgg ctgtcacgcg
     4681 gtatttcgtt tcgtcacgtc aaaactgacg acagcctgtt tttcgtcaga gttttgaata
     4741 aatagtgccc gtaatatcag ggaatgaccc caaataaaat gtggcataaa agatgcatac
     4801 tgtagtcgag agcgcgtatg cgtgatttga ttaactggag cgagaccgat gaaaaaagtc
     4861 gtcacggttt gcccctattg cgcatcaggt tgcaaaatca acctggtcgt cgataacggc
     4921 aaaatcgtcc gggcggaggc agcgcagggg aaaaccaacc agggtaccct gtgtctgaag
     4981 ggttattatg gctgggattt cattaacgat acccagatcc tgaccccgcg cctgaaaacc
     5041 cccatgatcc gtcgccagcg tggcggcaaa ctcgaacctg tttcctgaga tgaggcactg
     5101 aattacgttg ccgagcgcct gagcgccatc aaagagaagt acggtccgga tgccatccag
     5161 acgaccggct cctcgcgtgg tacgggtaac gaaaccaact atgtaatgca aaaatttgcg
     5221 cgcgccgtta ttggtaccaa taacgttgac tgctgcgctc gtgtctgaca cggcccatcg
     5281 gttgcaggtc tgcaccaatc ggtcggtaat ggcgcaatga gcaatgctat taacgaaatt
     5341 gataataccg atttagtgtt cgttttcggg tacaacccgg cggattccca cccaatcgtg
     5401 gcgaatcacg taattaacgc taaacgtaac ggggcgaaaa ttatcgtctg cgatccgcgc
     5461 aaaattgaaa ccgcgcgcat tgctgacatg cacattgcac tgaaaaacgg ctcgaacatc
     5521 gcgctgttga atgcgatggg ccatgtcatt attgaagaaa atctgtacga caaagcgttc
     5581 gtcgcttccc gtacagaagg ctttgaagag tatcgtaaaa tcgttgaaag ctacacgccg
     5641 gagtcggttg aagatatcac cggtgtcagc gccagtgaga ttcgtcaggc ggcacggatg
     5701 tatgcccagg cgaaaagcgc cgccatcctg tggggcatgg gtgtaaccca gttctaccag
     5761 ggcgtggaaa ccgtgcgttc tctgaccagc ctcgcgatgc tgaccagtaa cctcggtaag
     5821 ccgcatgcgg gtgtaaaccc ggttcgtggt cagaacaacg tgcagggtgc ctgcgatatg
     5881 ggcgcgctgc cggatacgta tccgggatac cagtacgtga aagatccggc taaccgcgaa
     5941 aaattcgcca aagcctgggg cgtggaaagc ctgcctgctc ataccggcta tcgcatcagc
     6001 gagctgccgc accgcgcagc gcatggcgaa gtgcgtgccg cgtacattat gggcgaagat
     6061 ccgctgcaaa ctgatgcgga gctgtcggca gtacgtaaag cctttgaaga tctggaactg
     6121 gtcatcgttc aggacatctt tataaccaaa accgcgtcgg cggcggatgt cattttgccg
     6181 tcaacgtcgt ggggcgagca tgaaggcgtg tttactgcgg ctgaccgtgg cttccagcgt
     6241 ttcttcaaag cagttgaacc gaaatgggat ctgaaaacgg actggcaaat catcagtgaa
     6301 atcgccaccc gtatgggtta tccgatgcac tacaacaaca cccaggagat ctgggatgag
     6361 ttgcgtcatc tgtgcccgga tttctacggt gcgacttacg agaaaatggg cgaactgggc
     6421 ttcattcagt ggccttgccg cgatacttca gatgccgatc aggggacttc ttatctgttt
     6481 aaagagaagt ttgatacccc gaacggtctg gcgcagttct tcacctgcga ctgggtagcg
     6541 ccaatcgaca aactcaccga cgagtacccg atggtactgt caacggtgcg tgaagttggt
     6601 cactactctt gccgttcgat gaccggtaac tgtgcggcac tggcggcgct ggctgatgaa
     6661 cctggctacg cacaaatcaa taccgaagac gccaaacgtc tgggtattga agatgaggca
     6721 ttggtttgag tgcactcgcg taaaggcaaa attatcaccc gtgcgcaggt cagcgatcgt
     6781 ccgaacaaag gggcgattta catgacctac cagtggtgga ttggtgcctg taacgagctg
     6841 gttaccgaaa gcttaagccc gattacgaaa acgccggaat acaaatactg cgccgtgcgc
     6901 gtcgagccga tctccgatca gcgcgccgcc gagcagtacg tgattgacga gtacaacaag
     6961 ttgaaaactc gcctgcgcga agcggcactg gcgtaataca gtcctttcta cagcctcctt
     7021 tcggaggctg tttttttatc cattcgaact ctttatactg gttactccct acccaatcgt
     7081 attatcaaaa tgaaaaaaat tatcgcattg atgttgtttt tgacattctt tgcccacgcg
     7141 aacgactccg agcctggcag ccagtattta aaggcagcag aggccgggga ccgacgtgca
     7201 caatattttc ttgccgacag ctggtttagc tccggcgatt tgagcaaagc cgaatattgg
     7261 gcacagaaag ccgccgacag cggtgatgct gatgcctgcg cgctgttggc gcagatcaaa
     7321 atcaccaatc cggtcagtct ggactatcca caagcaaaag ttcttgcaga gaaagcggcg
     7381 caagcgggca gtaaagaagg tgaagtaacg ctggcgcata ttctggtaaa tactcaagcg
     7441 ggtaaaccgg attatccaaa ggcgatttcg ctgttagaaa acgcctcgga agatctggag
     7501 aatgactccg ccgtcgatgc ccaaatgctg cttggtttga tttacgccaa cggcgtgggc
     7561 attaaggccg acgatgacaa ggcaacctgg tatttcaaac gcagctctgc aatttcccga
     7621 accggttatt ccgagtactg ggcgggaatg atgttcttaa acggtgagga aggttttatc
     7681 gagaagaaca aacaaaaggc gctgcactgg ttgaacctga gctgtatgga agggtttgat
     7741 accgggtgtg aagagtttga aaaattaacc aacggttaag gtaggcctga taagacgcgg
     7801 caagcgtcgc atcaggcatc cacacattgc cgggggatat ccccggcaat cttcaattat
     7861 tgatccgcag ttttatcaaa tttgcccagc acttcacgct cataagccag cgcctttttg
     7921 cggtcaaatt tgtgttccca cttggcaatc accagcaccg ccagcgcatt acccaccacg
     7981 ttcagcgcag tacgcgccat gtcgaggata cggtcaacac cagcaataaa cgccagacct
     8041 tccagcggaa tacctacgct acccagcgtt gccagcaaca ccacaaacga cacgccagcc
     8101 acgccagcaa tccctttcga ggtcaccatc agcgtcagca ccagaatgat ttcctgccag
     8161 atggacaggt caatgccata cagctgagcg atgaaaatag cggcaatact ttgatacagc
     8221 gtcgaaccat caaggttaaa agagtaaccg gtcggcacca cgaagctggt gatcgacgcc
     8281 ggtgctccgt aggcttccat cttctcaata attcgcggca gtacgctttc agagctggca
     8341 gtggagtacg ccagaatcag ctcatctttc agaatacgaa tcaggatcca gacgcttaac
     8401 ccgcacagac gtgccacaat tcccagcact accagcgcga agaacagaat ggcgaaatgc
     8461 accagcagca ccagtttcgc cagtggccac agagacgaga aaccaaagtt agccaccgtc
     8521 accgcaatca gcgcaaacac acccaccggt gcataacgca tcaccatgtg agtcacttta
     8581 aacatggttt cagagatgga gcggaacacg gtcaccagcg gttcacgatg cgtcgcgggc
     8641 agggaagaaa gccccagacc aaacagcacc gagaaaaaga tgatcggcag catttcgcct
     8701 ttcgccatcg acgccacaat gttcgtcggc accagcgaca aaatcgtgcc cataatgccg
     8761 tgggaactgc tttgtaccgc ttccgtagtg ctctgatatt tcgagatatc gacggtcgcc
     8821 agctgcgaca tatccacccc ggcaccgggc tggaagacgt tcgccagagt gatccccaaa
     8881 atgatggcga cggtggtgat cacctcgaag taaataatgg ttttcgcgcc aatacgcccg
     8941 agctgtttgg catcaccaac acccgcgata cccaccacca gcgtggagat cacaatcggc
     9001 acaacaatca ttttgatcag atggatgaag atatcacccg ccggagagag caaattgacg
     9061 accagccagt cgcggctgtc gctatggtag tgcaggtagc ttcccaggag aatgcccagc
     9121 accatagcaa acagaatctg ccaggccagg ctgaatttta tatttttcat cagaataact
     9181 tcctcaatgg agcgccgttc cccgttcaga aacgcgagaa cgtgacatac tgccagggca
     9241 tgaattgtgt tgcgttggtt tgtagatttt tttaacgctt atgatcagta tcatttgcgc
     9301 ggtatgttgc gcaacccttt aacaacagag cttttcgctg cattaatgca taataacgcg
     9361 agtttatggc aggattaatg aataaccatt tattatggaa agatttttat ttgtttaatc
     9421 tgcaataata ctgattggtg attatttttg gcatatgttc attcgattat tttttatgca
     9481 gctcaatgac ataatcttca tcaccagatg attaatccga tctccgctgc aacagttttg
     9541 ccatattaat cgactcgacc agctgtgtcc ggttgatccg gggtgagttg agatccatca
     9601 ctttttgcca tagttcgatg gcttgcgcgt aattcgcctg cataaacgca tcggaagcca
     9661 gcagcatcag agcggtgatt tcgttagagt ccagcgcgag ggctttgtcg atcattgcgc
     9721 gagtctgggc ggtcatatgc tggctggcct ggtaataaag caccgtcgcc agcgccgcat
     9781 acagttcagc gttctcacca cgtagttgca acgcctgacg atacgccagc agcgaattgc
     9841 tgtaatcgtt ttgccacaga tagtactcgc ccagtaacgc ccattgttcg ctgttttgtg
     9901 gattagcgcg gattttatcc tgcaatgcct gaagctgcgc ttccggggtt tgctggctgg
     9961 caaactgatg tagcggatcg cgctgacgct gatactccgc acgtaccgcc tgccattttg
    10021 ggcttaacag ataactgccg acacagagga ataccatcaa tatcgtgagt gttgtcagca
    10081 ttttcaccgg tatttgaggc tgtttcatcg cttagccctc actcgccaga ggatcagtgc
    10141 catcagcaat aacaacacca ctggcagcgc ccacaacacc agcgtctgac ccgttaacgg
    10201 tgggttatag cgaacaaaat ctccgtagcg ttcggtcatc cagccaatga tttcgacttc
    10261 gttcttcccc tccgacacca tgctataaac ctgatggcgc atactgaccg ccaccggcgc
    10321 attggattcg agtaagtttt gattctggca ctgcggacaa cgtaactggc tggcaatatt
    10381 taacgcctgt tgctgttgtt gcggattggc gaattgccag gtgtctacaa cctgagcgcg
    10441 cgcaaaacag gtaaataaca gcagtagcgt gagaagccct ttattcatcg cgcttcttcc
    10501 cccgccatcc gcttaacaat gcgcccgcaa tcatcaacaa tcctcccccc cagatccagc
    10561 gcacaccgct ttgtacatac aaacgaaaag cgtaacgatc cgctctggtt ttttcaccca
    10621 tgaccgcata ccagtcatgg atgccgttcc agcgaattga cggttccatc atttgctgac
    10681 ggcgtgcttc ataaaaacgc cgctccggcg ttaattcacc aatgcgttgc tgatggtcaa
    10741 acagcgccac tatcgctttt tcgctggtgt aattgcctct ggcttgcaga tcgagtcgct
    10801 caaaacggaa ggtgtatcct gccagcgtca cctgctgacc cggctgtaaa ttcaggctga
    10861 tctcctgacg actgacactg gaaaccacga tccccgcggc aaataacagc acgccagcat
    10921 gagctaccag cgccggaagc tgcacgcgtt tgccagagac aaacgtcgcc agcacaatca
    10981 ccaccagcat caacagacca aacggtaacg tcgcgcggtt aaaatacggc gcgccaacgg
    11041 agaggcgtcc ccagcccagc aggccgtaaa tcatcggata aagcgttccc accagcacga
    11101 tcagtagcac tgcgcaaaac agcaacagcg tagcgaggat taacatttcc cgcgataacc
    11161 ccgaaaaacg caccgccggg ccaccgtccc gcgctcgcca gccatacaga gccaaagacg
    11221 caaggctaat cagtgcaaac aggctgaaca acggcacggc gcggacgtta tccagtgcga
    11281 acgcatgaac cgaaaccaga atgccagaac ggacaattaa ggtgcccagc agcgacagca
    11341 tcagagtaac tatcgccagc aacagcgacc agtggcggaa aatcccccgc tggcgtgtca
    11401 gggacaaact gtgcagcagc gcagtggcag aaagccaggg taataaagag gcgttttcca
    11461 ccggatccca gaaccaccag ccgccccagc cgagttcgca gtaggcccac caggaaccga
    11521 ggatgatccc cgccgttaat gcactccagc caggtaacgc ccagcgccag caaattcggg
    11581 cgctggccgc atcaaactcg ccgcgcagta gactcgccag cgccacgctc gccgctacca
    11641 tcaaaccgcc atagccaagg caaagcagcg gtggatgaaa gataagaccg ggatgttgca
    11701 gcatcggatt gagatcgcgg ccttcgattg ctggtggaaa tatccgcacg aagggatcgg
    11761 accacagcac cacaaacagt agcagtgcgg cgagcatgag agataaaacg gctagcgtca
    11821 gcggaaatag cggatcgctt tgctgccgat aatgccaggc aaacagcgcg ctccaggcgg
    11881 aaagcagcag cacccaaagc agcagcgaac cttcatgacc gccccacacc gccgccagtt
    11941 tgagttccca cgacagcagg ctgtagctat gttgggcgac ataaatgacc gagaaatcgc
    12001 tgatgagaaa acaatacgtc agtacgccaa aggcgagcag caggagcgcg aactgcgcca
    12061 gaatgccgat gcaagtgagt cgcatcatgg caggccagcg caaccgcact cccgcaaagg
    12121 ccgtcaacgg ggtcaacacg ttgaccccga gacttaacaa caacgccaga aaaccgactt
    12181 caggaaggaa tacgtccaga aaccacctct tttctggctt acgccaccgt caactgtccg
    12241 gcgtagagaa tgaaaaagcg cagcatcaac acgccgacca gactggcccc acacgccgcc
    12301 agcacggcag gaatgccgga actgcgattg acccacggtt tgagcaacat tggcacaatc
    12361 agccccagcc cggcgacacc aagccagaac caccacgtcc agaaaccgcc acctaatgcc
    12421 gccaccagcg cacgaacttt accgtcatcg cccagcgcca gaccgacaaa aaacgccacc
    12481 agcaggaaga tttcacccca taccacgggg atttccatcc ggtgaacaaa gtgcgcttcc
    12541 gtggaatgcg ggttactgcg ttggcgtatc gccatggcga tcagcgccac cgccgcaccg
    12601 gacgagatgc cggagaagag gagcagcacc ggcaggctcg ggttattgag gaacggatac
    12661 gatttcagcg ccgacagcag aaagccggta taagccccca gcagcaccgc caacaccagc
    12721 atcagcgttt ccagtccgcg atgaacgggc gtcagcaggc tcagaacctt ttgcacgatc
    12781 cccagcttcg gcaaccagcg ttgttgcagg gcaagcaaat catgttcaaa gattttcgcc
    12841 agccacagca ccagcaccac catgtagagc tgaaacagca tcacccccat cgacatcacc
    12901 gaggtaaaac tgtagtggaa catcagcttc cagaaggtcc acggtcttgt caggtggaag
    12961 acgaggatca acagaccgag gatcaccgcg cccggcccga caatcagcgt ggtgcgcagc
    13021 aacgtactgt ctgcaccgcc cgcctgcggg tagaagcgac gtaacagcac ggccagcgtc
    13081 accagaccgg cagaaatacc aatcaaaaac aggtagatgg caatcggcca gtcccacacc
    13141 agcgattcaa aatgaaatgc ggaagtctgc gtcattggct cacctccccg tatttaaacg
    13201 gtacgcggta cagcttcggt ttggttccca gcgccagctt gtagcggtaa gtaggcttct
    13261 ggcgcagcag ttgcgaaatc tcactgttag gatcgtccag attgccaaac gtcagcgcct
    13321 tcgtcgggca agcttcaacg cacgcgggaa gcttaccggc ctgcaaattg gtcttacgac
    13381 agaaatcgca tttgtccgcc gtcttcgtga ccggatggat aaagcgcacg cggtacggac
    13441 aggcggcgat gcagtactga caaccgacgc agagatcagg attaacatcg acaatcccgc
    13501 tagcggcatc acgaaaagac gcaccggtcg ggcagacgtc aacgcacggc acatgatcgc
    13561 agtgctggca agacttacgg aagaagcgat atttcacatc aggaaattcg ccctgcggct
    13621 cgctacgaat aatcgtcagg cgcgagacgc cttccggcac cttgttcact tcccgacaag
    13681 catccataca ggcggtacag ccgatgcata acgactcgtc atgcaccatg ccgtaacgca
    13741 ccccattgat attcaacgtt ttcgccacca cgcgccctgc ggtgccgctg acggctgcca
    13801 gcacgccgac gccggtgaga aactggcgac gagaccaggt catggctgct ccttaagcaa
    13861 cggaacagac gccgggttaa agttcggatt ggtgcgctga tcgctgtggc aatcgacgca
    13921 aatcttaatc cgtcctttgt cacttaacgt ctgcatcgta tcttgctgtg gatgcaggga
    13981 atggcagctg gcgcacgcca ctttggttac gtggacatcg tgcggccaga acgctttttg
    14041 caactgttcc ggcagatgac aggacataca gacgctgttc tgctctccga ccttatacat
    14101 cggctcgtta aagcgcatca catctttcac cccttcgcgg tgttgcggtg atgcttggcc
    14161 gtgacagttg gtgcaggtga ccggcaattt gttattcggg ttgataacgg aagcatgttt
    14221 gccatgcata ccttcggtat ctggtttatg acagtccaga caggcggcat ccgggttacg
    14281 ctgctgggta acttcgtagc gatcaccaga tgcctgcacc gcaggggtag cggtaattcc
    14341 gttcaggctc cacaacaggc ccgacgccag caccccagca gttaataacg aacgtaatac
    14401 gctcatattc actccattgt gttttccgcc ccttgcgagg cggaacgggg ttattggctt
    14461 aacagaccgt ttttacgtgc ctgctcttcc cactgcggga tcaccgtttt gatgaagtcc
    14521 tgcttctcgg ccttgatttg ttccatgttc aggccaatgg cctgctgggc tttctctttg
    14581 gtcgagatat ccgggatctg gatttcatgg gtgatacctt tggtcgccag caggcgcgcc
    14641 agtttggtgc gcgcatccgc cgctttatcc atcgccgtac cgagcatccg tagaccttct
    14701 tccggtgcgt gcatatgaat accgtgggaa gcgatcgcca gatcccaacg ccactgggca
    14761 tgacggatat cgtcctgaat cggcttcatt tcggcttccg tcgcgcctgc atccagcgcc
    14821 gctttcgctt cgaagtgagc gtgaaccagt tgatcttcaa ccttgatttt caggtcgtta
    14881 atcgattgct tacgttccgc gaccactttt tgcaaggcag ctttatcctg ggtatggcag
    14941 ttcgcacaag tctgggcgaa gttatcaaac ggattaccaa ttttatggtc ggtgtagagt
    15001 ttgccttcgg cgttctgcac ttttggcata tggcagtcga tacaggtcac gttgttttta
    15061 ccgtgaatgc ccgctgtcca ggtttcatat tccgggtgct gcgctttcag cattggcgtt
    15121 ttcgacaggg agttagtcca gtcagagaag gcaattttgt cgtaatactg ctccatattt
    15181 tcgactttca tgccgtcatc ccacgggaat ttaaccgctt tgtttttgcc gtcgaagtaa
    15241 tactccacat ggcactgacc gcaaaccatc gattgctggt cgaaacgtcc ggctttctca
    15301 aaaggtttac caatggcttc catcgcgcga gccgcatacg gacgggaaag ggttaactcc
    15361 ggtttgcctt tggcgaactc tggtgaggcg gtgttatggc agtcggcaca acctaagttg
    15421 ttgacgattt ccgggccgcc gcgcgcccat ttaccgtgga agtagccatc ttcgccgtct
    15481 ttctggatca gacgcgccac atccgggctt ttacaactcc agcacgccat cggtagcgga
    15541 ccatcttcag cgtttttcgg cgcaccggta cgcagggttt cacgcacatc ggtcacagca
    15601 aaggcatgtc cacgaggctt gttgtaatcg cgcgagaagg gataccccgc ccacaggatc
    15661 accagccgtg gatcttccgc cagggcgtca acacgctctg actgttccga ggtggctttc
    15721 caggagagat attgatcgtg atgctgcggg gcaaaggttt cattcttcgc ttccacagtt
    15781 acaggttttg cgggagctgc cgtttgttca gcgtgaacag aagtgaaaaa gaaaaaagga
    15841 atcaataagc tgaagatacg gcgtgcgttt atttttatcc ttgtcatagg ggcttcatcc
    15901 gaattgcgcc attgttgcaa tggcggtttt tattgttttt cacgacagta accgcaccta
    15961 cactgtcatg acattgctca cccctatgtg taacaaataa ccacactgtg aatgttgtct
    16021 ttaatcaatt gtaagtgcat gtgaaatacc actttagagt tagtcagtat cttcctcttt
    16081 ttcaacagca tgcataactg catgttcctc aaagaattaa tcaacttttg ttggtgacct
    16141 tcaaaaatta ccctgccgtt tatttgcacc attctacttt tgcgtgatct gtcgcccaaa
    16201 tactaaacaa aactgccaat acccctacat ttaacgctta tgccacatat tattaacatc
    16261 ctacaaggag aacaaaagca tgagccaaat tcacaaacac accattcctg ccaacatcgc
    16321 agaccgttgc ctgataaacc ctcagcagta cgaggcgatg tatcaacaat ctattaacgt
    16381 acctgatacc ttctggggcg aacagggaaa aattctcgac tggatcaaac cgtaccagaa
    16441 ggtgaaaaat acctcctttg cccccggtaa tgtgtccatt aaatggtacg aggacggcac
    16501 gctgaatctg gcggcaaact gccttgaccg ccatctgcaa gaaaacggcg atcgcaccgc
    16561 catcatctgg gaaggcgacg acgccagcca gagcaaacat atcagctata aagagctgca
    16621 ccgcgacgtc tgccgcttcg ccaataccct gctcgagctg ggcattaaaa aaggtgatgt
    16681 ggtggcgatt tatatgccga tggtgccgga agccgcggtt gcgatgctgg cctgcgcccg
    16741 cattggcgcg atgcattcgg tgattttcgg cggcttctcg ccggaagccg ttgccgggcg
    16801 cattattgat tccaactcac gactggtgat cacttccgac gaaggcgtgc gcgccgggcg
    16861 tagtattccg ctgaagaaaa acgttgatga cgcactaaaa aacccgaacg tcaccagcgt
    16921 agagcatgtg gtggtactga agcgtactgg cggaaacatc gactggcagg aagggcgcga
    16981 cctgtggtgg cacgacctgg ttgagcaagc cagcgatcag caccaggcgg aagagatgaa
    17041 cgccgaagat ccgctgttta ttctctatac ctccggttct accggaaaac caaaaggcgt
    17101 actgcacact accggcggtt atctggtgta cgcggcgctg acctttaaat atgtctttga
    17161 ttatcatccg ggtgatatct actggtgcac cgccgatgtg ggctgggtta ccggacacag
    17221 ttatttgctg tacggcccgc tggcctgcgg cgcgaccacg ctgatgtttg aaggcgtacc
    17281 gaactggccg acgcctgccc gtatggcgca ggtggtggac aagcatcagg tcaatattct
    17341 ctataccgca cccacggcga tccgcgcgct gatggcggaa ggcgataaag cgattgaagg
    17401 caccgaccgt tcgtcgctac gcattctcgg ttccgtgggc gagccaatta acccggaagc
    17461 gtgggagtgg tactggaaaa aaatcggcaa cgagaaatgt ccggtggtcg atacctggtg
    17521 gcagaccgaa accggcggtt tcatgatcac cccgctgcct ggcgctaccg agctgaaagc
    17581 cggttcggca acacgtccgt tcttcggcgt gcaaccggcg ctggtcgata acgaaggtaa
    17641 cccgctggaa ggggctaccg aaggtagcct ggtgatcacc gactcctggc ctggtcaggc
    17701 gcgtacgctg tttggcgatc acgaacgttt tgagcagacc tatttttcca ccttcaaaaa
    17761 tatgtatttc agcggcgacg gcgcgcgtcg cgatgaagat ggctattact ggatcaccgg
    17821 gcgtgtggac gacgtgctga acgtctccgg tcaccgtctg gggacggcag agatagagtc
    17881 ggcgctggtg gcgcatccga agattgccga agccgccgta gtaggtattc cgcacaatat
    17941 taaaggtcag gcgatctacg cctatgtcac gcttaatcac ggggaggaac cgtcaccaga
    18001 actgtacgca gaagtccgca actgggtgcg taaagagatt ggcccgctgg cgacgccaga
    18061 cgtgctgcac tggaccgact ccctgcctaa aacccgctcc ggcaaaatta tgcgccgtat
    18121 tctgcgcaaa attgcggcgg gcgataccag caacctgggc gatacctcga cgcttgccga
    18181 tcctggcgtg gtcgagaagc tgcttgaaga gaagcaggct atcgcgatgc catcgtaacc
    18241 cacaattgcc ggatgcgaat cggtaacggt ttgtaggcct gataagacgc gacagcgtcg
    18301 catcaggcat tgattgccgg atgcggcgta aacgccttat ccggcctaca ttcggcaagg
    18361 gttacccgag cgttaacctt ctcccataag ggagcgggaa ttaaaacaat ccctacatta
    18421 cctctggaga atctgtgatg aatggtacga tttatcagcg gatagaagac aatgcgcatt
    18481 tcagggagtt agtcgaaaaa cggcaacggt ttgccaccat cctgtcgatt attatgctgg
    18541 cagtttatat cggctttatt ttactgatcg ccttcgcgcc cggctggctg ggcacgccgc
    18601 tgaatccgaa caccagcgtc acacgcggta ttccaattgg tgttggagtg attgtgatct
    18661 cctttgttct caccggtatc tacatctggc gggcgaacgg cgaattcgac cgtcttaata
    18721 atgaagtcct gcatgaggta caagcatcat gaaaagagtt ctgacggcgc ttgccgccac
    18781 actccctttc gcagctaacg ccgcggatgc tattagcggg gccgtagagc gccagccaac
    18841 gaactggcag gcgattatta tgttcctgat tttcgtcgtg tttacgctcg gcattaccta
    18901 ctgggcatca aaacgcgtac gttctcgtag cgactactac accgcaggcg gcaatatcac
    18961 tggcttccag aacgggctgg cgattgccgg ggactatatg tccgccgcct cattcttggg
    19021 gatctccgcg ctggtgttta cctccggcta tgacgggctg atctactcgt tgggcttcct
    19081 ggtgggctgg ccaatcattc tgtttctgat tgccgaacgt ctgcgtaacc tgggacgcta
    19141 cacctctgcc gatgtggcct cttatcgtct gaaacaaggg ccgattcgta ttctttcggc
    19201 ctgtggttct ctggtagtgg tggcgcttta ccttatcgct cagatggtag gcgcaggtaa
    19261 actgatcgag ctgctgtttg gccttaacta tcacattgcg gtggtgctgg tcggcgtgct
    19321 gatgatgatg tacgtcctgt tcggcggcat gctggcgacc acctgggtac aaattatcaa
    19381 agctgtgctg ttgctgttcg gtgccagctt tatggccttt atggtgatga aacacgtcgg
    19441 ctttagcttc aacaatctgt tcagcgaagc gatggcggta cacccgaaag gtgtcgacat
    19501 catgaaaccg ggcggactgg tgaaagatcc gatctccgcg ctctctctgg ggctgggact
    19561 gatgtttggt acggcgggct tgccgcacat tctgatgcgc ttctttacag tcagcgatgc
    19621 ccgcgaagca cgtaagagcg tgttctacgc caccggattt atgggctact tctatattct
    19681 gacctttatt atcggcttcg gcgcgatcat gcttgttggt gcgaatccgg aatataaaga
    19741 cgcggcgggc catctgattg gtggtaacaa catggcggcc gttcacctgg cgaatgcagt
    19801 gggcggcaac ctattcctcg gttttatttc agcggttgct ttcgccacta tcctcgcggt
    19861 ggttgcggat ctgacactgg cgggcgcatc ggcggtttcg catgacttgt acgctaacgt
    19921 cttcaaaaaa ggcgcgaccg aacgtgaaga gctgcgggta tcaaaaatca ccgtactgat
    19981 cctcggcgtg attgcgatta tcctcggcgt gctgtttgag aatcagaaca tcgcctttat
    20041 ggtaggcctt gcgtttgcca tcgcggcgag ctgtaacttc ccgatcattc tgctttccat
    20101 gtactggtcg aaactgacta cccgtggcgc gatgctgggc ggctggctgg ggctgattac
    20161 cgcagtggtg ctgatgatcc tcggcccgac tatttgggta cagatcctcg gtcacgaaaa
    20221 agccatcttc ccgtatgaat acccggcgct gttctctatc agcgtggcat tcctcggcat
    20281 ctggttcttc tcggcaaccg ataactcagc ggaaggggcg cgcgagcgtg aactgttccg
    20341 cgcgcagttt atccgctccc agaccggctt tggcgttgag caaggccgcg cacattaatc
    20401 tctccccttc cccggtcgcc tgaccgggga atacgcttcc tctccaacat gcatcacctt
    20461 ttcccataat attaaacaaa taaactcatt aaaaaatgag cgatttttga cagtcgtaga
    20521 aaatgataat gcagagaata tgccttttct ttcttgttaa ttataaggat attttatgcg
    20581 ctataatggt ttaaataata tgtttttccc tctttgccag attaacgata accactctat
    20641 cacaagttca tcacatacaa agaaaacaaa atcagataat tacagcaaac atcataaaaa
    20701 cacgttaatt gacaataaag ccctctctct tttcaaaata gatgatcatg aaaaagtgat
    20761 aggcttgatt cagaaaatga aaagaattta tgatagttta ccatcaggaa aaatcacgaa
    20821 agaaacggac aggaaaatac ataaatattt tatagatata gcgttatatg caaataataa
    20881 atgtgacgat agaattacga gaagagttta ccttagtaaa gaaaaggatg tatccattaa
    20941 ggtggtatat tttataaata atgtcgccgt ccataataat actatcgaaa ttccacagat
    21001 agtaaatggt ggttacgatt tttcacacct tagcctgaag ggtatcgtga ttaaagatga
    21061 agatttatcc aattcgaatt ttgcaggttg cagactacaa aacgctattt tccaggactg
    21121 taatatgtat aaaacgaatt tttattacgc cataatggaa aaaatacttt ttgataattg
    21181 tattctcgat gactcaaatt tcgctcagat aaaaatggcc gacggaactc taaatgcatg
    21241 ctccgctatg catgttcaat tctacaatgc agcaatgaat agagccaata ttaaaaatac
    21301 cttccttgac tattcaaatt tttatatggc gtacatggct gaggtaaatc tttataaagt
    21361 aatagcgcca tatgttaatt tatttaaagc cgaccttagt ttctctaaac tcgatttaat
    21421 taactttgaa catgctgatc tgtctcgcgt caatctgaac aaagcaatcc tccagaatat
    21481 aaacttaatt gatagcaaac tcttttgtac gtggctgaca aatacattcc tcgaaatggt
    21541 tatatgtacc ggctctaata tggctaatgt taattttaat aatgccaatt taagcaactg
    21601 ccatttcaac tgttctattt taacaaaagc ctgtatgttt aatacccgtc tctatcgggt
    21661 taatttcgat gaggctagcg tccagggaat gggcatttcc attctccgtg gggaggaaaa
    21721 tatccccatt gatagtgata ccctggtaac actacagaaa ttctttgaag aagattgtac
    21781 ctctcatact ggcatgtcac aaactgagga taatattaat gcagtcgcta tgaagattac
    21841 tgcagatatt atgcaacacg cagattgagg ttaagtcgaa gaacagccaa tgaattgact
    21901 ttacaagaga gataacttat ttgttttaga atcatgaatg ttcgcccgag ctaacctgtt
    21961 aaaggtgcaa aagattgcac ctttttcaga ttacctcggg cctggttacg tcgctttcgg
    22021 cttactgatt ttcctcaatt agcaacgctt caagcaaatc gagatcgtgc agtaattttt
    22081 gcagcgtttc gttgctgatc tcccgcgtgg cgcgcaggtg gtacagttca gcacgttcag
    22141 aacgcaatgc cgccagacgg aagcgacgct caaggttctc ttcctgcacg gaactttcaa
    22201 cgtcgttacg tccatcggcg cgacgacgca ggttaccaat gacgcgagaa ctgacctccg
    22261 taagcaactg gttatcgata ttctcttcgg tatccgccgc cagacgctcc tccattttct
    22321 ggatcgccac aatcgccact tctgccgttg ccgctcgcgc aatacgttct tctttcaatt
    22381 gttgcgaatg gtccgcgact tcaatgtgtt gtagcagaat aggcaacatc accacgccga
    22441 caaacagcga gaagagaatg acgccagccg ccaggaacac cagctcatag cgcgccggga
    22501 agacgttacc atccggcaag agcagcggga ttgagagcac accggccaga gtgatcgccc
    22561 cacgcacccc agcgaaagac gcgatcagga tttctcgtgt ggtccacgaa ccaaactcca
    22621 tcggcttctt cttcaggaag cggttgctga actttttcat cgtccacagc cagccgaaac
    22681 ggaccagcat cagcgccgca tatatcagaa taatatcggt aaacagcatc cagatttcga
    22741 cgttagggtc gatttctgcc gccatcagcg acgtctccag aatacccggc agttgcagac
    22801 ctaacagcag gaacaccatg ccgttaaaga caaattccag catcgcccag gtgctgtttg
    22861 cgcgcaggcg cattgccagt ggcgcacggc gcatcacacc ggagcgggtg atggtcatcc
    22921 cggcggcaac cgcagcgagg atgcccgaaa cgccaatatg ttcggcaatc agataagaag
    22981 cgaatggcag caagaacagc agaacgatct gcgtcgcggg ttcatcaccg ccccagcggc
    23041 tgaggaatcg cagcgagcga ccatacagcc agctcaccac aaaaccggca agaataccgc
    23101 caatggctac tttcataaat tcgaccgtcg caccgccgac agtgaagatc atcgtcccca
    23161 tcgccactgc cacggcaaac ttcaacgaca ccaggccgga agcgtcattc atcaacgctt
    23221 cgccctgcaa aatgcccatg atttttttcg ggatgcgccc ttcgccaaca atcccggaga
    23281 gcgccacagc atcggtcgga gaaagtaccg ccgccagcgc aaaggcgggg atcagcggaa
    23341 tgcccggcac cacccagtaa ataaggaagc caatgccgac cacggtgacc accaccagcg
    23401 ccagcgcgag gccgaaaatc tctcgaccat gttcgagaaa ttcacgggtc ggcgttttcc
    23461 agccatcagc gaacagcaac ggcgggataa acaagactaa aaagagttca ggatcaaact
    23521 ccacatgcaa accaaacgtc ggccacgcca gtagcgcacc gatggcgatt tgcataagcg
    23581 gaagcgggat ctgaaagggc atgacacgag tgaccacccc ggacagcgag accacgaggg
    23641 tcattatcag tatggtgaag aagatttcca tgcgttccct gtttgcgatg ttttttatga
    23701 tctgagaaag acgttatgcc tgaattctca ttttttcaga atatcgcaaa agctcacaca
    23761 atgtgcggag aaaataaaaa cgggcagcca ttgccacccg ttttcagaat ccgatgaaag
    23821 attagatagc ccagccaccc gcatagaagg tcaccagcgc cacggcgata acgaccgtac
    23881 cgatgttcaa cttgcgccat tcaccggaaa ccagacgacc aatcaccaga gtcgcgaagc
    23941 cgatcatgat gcctgttacg atgttacagg tcagcacgat gaataccgcc gtaaccagac
    24001 ccgccatcgc atcaacaaaa tcagcaaagt cgattttcgc cacgttgctc agcatcagca
    24061 ggccaacgta catcagcgcc ggagccgttg cataccccgg aacgaggtaa gaaagcggag
    24121 agaggaacag aatcagcagg aacagcacgc caacggtgat agccgtcaac ccggttttac
    24181 cgcccgccgc cgtacccgcc gcagactcga tgtataccgc tgccggagct gcacccacca
    24241 ggccagagaa aacgctgctc atggagtcag tggtcagtgc tttcccgccg tcgatgatct
    24301 gcccatcttt atccagcagg ttcgcctggc cggcgacagc gcggatagtt ccggtggcat
    24361 caaatactgc cgtcatcacc agcgccagaa cgcttggcag gactacagga ttcagcgcgc
    24421 ccataatgtc caggctgcca atcagtgaat tgccgttttc atcgctcaat gaaggcatgg
    24481 cgaaaacgcc ggagaaatgg acgttaggat cgaagatcaa accgacaatt gagataccga
    24541 taatggtcag cagaatgcca ccagggactt tcagtttttc caagccgatg atcaccgcca
    24601 gacctaccag tgacataatc accgggaagg tcgcgaaatc acccagcgca acgggcagac
    24661 catcaagcgg gtttttaatc accagaccga caccattagc ggcaatgagc agcaggaaca
    24721 gaccgatacc gatccccgtg ccgtgcgcca caccgtgcgg caagttgcgc aaaatccagc
    24781 tacggatacc cgtggcagaa attaccgtaa acagcacacc catcaggaac acggcaccca
    24841 gcgcgacagg tacgctaata tgttgcccca gcaccaggct gaatgcggta aacgccgtca
    24901 gggagatggc gcaaccaatc gccaacggca gattagccca cagacccatc acgatagaac
    24961 cgagtccggc aaccagacag gttgcaacga aaactgccgc aggcgggaag cctgctttac
    25021 ccaacatacc tggaacgacg atgaccgagt agaccatcgc cagaaacgtt gttaacccgg
    25081 caaccacttc ctgacggaca gtgcttccac gttgtgaaat tttaaaccag gcgtcgagtg
    25141 aaccgccggt acgcgctgat ggcgtagaca taagaaaaca tcccctgaga gtttaatttt
    25201 cgtcaagatg cgtggcggac aatcccctgc cagcgaaacg ttatcttcct gctccaggtt
    25261 tgcggcaatt tggtgcgtcg caaaaaagca aacgtttagc tccgcgcata caaaacgggt
    25321 ggtaaaaaaa agcaaacgat tatccggcga tcatagcggc tttttcaacc gaagtttgct
    25381 gcttttttct cttttattgt cttgacgatg aaaaaccagg caattgatgc gcaaacgtta
    25441 tcgtcgatgt gcaagattgc agcgttatcg agccgtatac cgttctggat tgcgataaaa
    25501 aatcgtctga gatgacaaaa atattgcggc taaaaagtat acttttacac tggcgaagaa
    25561 acgtcggtga catacaatta aagcatcaac accaaccgga acctccacca cgtgctcgaa
    25621 tgaggtgtgt tgacgtcggg ggaaaccctc ctgtgttcca gcgggataga gagaaagaca
    25681 aagaccggaa aacaaactaa agcgcccttg tggcgcttta gttttgtgtt tgcggataat
    25741 tccaaaagag ctcgatcagt cccctcgccc cttcggggag agggttaggg ttaggggaac
    25801 aggcagcacc agtgcaaata tttaccctca ccccggcccc atagttaacg atgagtcagc
    25861 ttgtccttgt ggcgctttag ttttgttcat cttccagcaa gcgtgcgccg gtaccttctt
    25921 ctcctaagcg gtcgcccggg ttacgcaacg ggcaatcact gcgcgaaagg cagccacaac
    25981 caatacatcc gtccagttcg tcacgcagcg ccactaaggt atgaatgcgc cgatccaact
    26041 cttctcgcca ttgggacgaa agctgtttcc actctttcgc acttaacgta tgcccttctg
    26101 gcaacacgcc aaacgcttca ccaatggtcg ccagcggaat gccaatacgc tgagcgattt
    26161 tgataattgc gacatatcgc aacacatcac gtttatatcg ccgctgattg ccgctgttac
    26221 ggatactagt aatcaaccct ttgctttcat agaaatgcag cgccgatacc gccacaccgc
    26281 tgcgtttcgc cacttcgcca ggggttagca gcgctttaat gcggggtaat ttcttttcca
    26341 taaatcgctt tacctcaagt taacttgagg aattatactc cccaacagat gaattaacga
    26401 actgaacact gaaaagaggc agatttatgt cccatcagaa aattattcag gatcttatcg
    26461 catggattga cgtgcatatt gaccagccgc ttaacattga tgtcgtcgca aaaaaatcag
    26521 gctattcaaa gtggtacttg caacggatgt tccgcacggt gacgcatcag acgcttggcg
    26581 attacattcg ccaacgccgc ctgttactgg ccgccgttga gttgcgcacc accgagcgtc
    26641 cgatttttga tatcgcaatg gacctgggtt atgtctcgca gcagaccttc tcccgcgttt
    26701 tccgtcggca gtttgatcgc actcccagcg attatcgcca ccgcctgtag ttttattgcc
    26761 cgcgcgttaa ctcccgcgcg ggtaattgct ccatccattg cataaacacc tgcggcggca
    26821 tcgccttcgc aaaaaaccat ccctggcaat agcgcacgcc gcgtttgcgc agccagttaa
    26881 cctgctcctc agtttcgacg ccttcagcga tcgtttttaa ccccaggctg tgcgccagct
    26941 cgatgatgtg ttccgcaatc aaatgactgg ttttgtgggt ggtcagcgtt tcaacaaacg
    27001 atttatcgat tttcaaaata tcgacattca atgatttaag gttatgcaag ttagagtagc
    27061 caataccaaa atcatcaatt gccacttcgt aaccagcctg gcggaaagcc agaattaatc
    27121 ggcgtcattt tttcgacatc aagaaatgca tgctcagtca cttcaaattt aatttgctgc
    27181 ggacgcaccg cgtattgctc tgttttctga ttgattcgcg ctatcaaccg tgacgtatga
    27241 aaatcggagg ccgacaggtt aatagaaaca tagcgatcgg catgtgttgc caggtacgcg
    27301 cccagatcgc ggaagacatt atcaataaca taatcagtta tctgttctat catcccctcc
    27361 ttttctgcca gcggaataaa ctctgccgga ttcattattt gcccctgctc accaggccaa
    27421 cgtaacaacg cttcagcgcc gatacatttt tctgttttga tatcgattat tggctggtaa
    27481 taaagacaaa gttgatgttt ttcgagggcg cgttgcaatt tacgtttggg agataaatag
    27541 ttttgtcgaa tacgtagcca gagtaatagc agaacaagac tccccaaaat accggcgggc
    27601 aacgcaaaaa taagatgatt ataaaaatgg gttataagac gttgatatga agtcgcaaca
    27661 atggctgcaa ttgggcgttt tgtcgaataa attgtcgcat ataaatagcc atttctttgt
    27721 acggttaaat ccttcaaatg aatcagcgga gaaaacgttg ctgccgaggc ctctttgctt
    27781 aacgagaaaa aggttttcgt caccgtatca tacacacccc attgcaatgt cgggtcatca
    27841 gacatcactt cactccagaa gagagggttg ataaccgcca cataatttcc ccgctgcata
    27901 taggtcattt tatagccaga gaaaaaaggc gtatcgcggt aataatagat agaaacgtta
    27961 ggttcacgct tataatcggc cggtgcaatc gtatagccgt ttacaggcgc tatcagcgat
    28021 gagcataaaa aatggttatc acgaattcat taatataaag atagccacga ataatattca
    28081 acattcgctt ttgatgggct ggagtgcaaa cttgcccctg atagcgttca gctgcatcgc
    28141 tcactaaatc tgcctggcga atgaccagct cggatttatc cagtgccagt tgagcaaagg
    28201 ttcggagctg attattcact tctgattttg cccaaaggaa ggcaatccat agcgaaagaa
    28261 tgatgggaaa gagaactaaa aagataatgc ccggcaacgc cagtaattgg tgtcgtgcac
    28321 gatgactcat tgctatcaat ttcctgcctg tttataccca gccattgcaa ggtgaaaccc
    28381 tggctaccga cgtctttaga taaatcttac ggtataaata gcacttttgc ctgatttgaa
    28441 attgatacct ttttaatcgt cacctggatg aaaataattc attgatattc aaaattaatt
    28501 ttctacagga atacagaggc ggcgggaaga tatgccctta atgccactga aaaaccacat
    28561 aatttgtaca gcaataaaag tcacggccta atatggctaa caaacaatca attgtgatat
    28621 agttcacaaa attaatgaaa caaacagagt gtttcatttt tgtgttctgc aagccccgcg
    28681 atagccagag tgtaccacgc ctcccgtgaa caacgccgcg ctgtccaggg catcggctct
    28741 tttcagagga attgtttgat ggctaccctc accactggcg tggttcttct tcgctggcaa
    28801 cttcttagtg ccgtaatgat gtttctggcc agcacactca acatccgttt tcgtcggtct
    28861 gattatgtcg ggcttgcggt gatcagcagc ggtctgggcg ttgtttccgc ctgttggttc
    28921 gcaatggggt tgcttggcat cacaatggcg gacatcaccg ctatctggca caacatcgag
    28981 tcggtgatga tagaagagat gaatcagaca ccgccacaat ggccaatgat tttgacctga
    29041 tacgtagaag c
//
LOCUS       DAEEUE010000046        28425 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.046, whole genome shotgun sequence.
ACCESSION   DAEEUE010000046 DAEEUE010000000
VERSION     DAEEUE010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28425
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.046"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            564..1334
                     /locus_tag="KLK84_002164"
     CDS             564..1334
                     /locus_tag="KLK84_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HBE2009479.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1325..1798
                     /locus_tag="KLK84_002165"
     CDS             1325..1798
                     /locus_tag="KLK84_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HBE2009480.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPRLWLYRKRPRV"
     gene            1905..2444
                     /gene="dnaT"
                     /locus_tag="KLK84_002166"
     CDS             1905..2444
                     /gene="dnaT"
                     /locus_tag="KLK84_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HBE2009481.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2447..3184
                     /gene="dnaC"
                     /locus_tag="KLK84_002167"
     CDS             2447..3184
                     /gene="dnaC"
                     /locus_tag="KLK84_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HBE2009482.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3233..3727
                     /locus_tag="KLK84_002168"
     CDS             3233..3727
                     /locus_tag="KLK84_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HBE2009483.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3981..6272
                     /gene="opgB"
                     /locus_tag="KLK84_002169"
     CDS             3981..6272
                     /gene="opgB"
                     /locus_tag="KLK84_002169"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HBE2009484.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="KLK84_002170"
                     /pseudo
     CDS             complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="KLK84_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7571..8485
                     /locus_tag="KLK84_002171"
     CDS             7571..8485
                     /locus_tag="KLK84_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBE2009485.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8700..10061
                     /locus_tag="KLK84_002172"
     CDS             8700..10061
                     /locus_tag="KLK84_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE2009486.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10110..11774)
                     /gene="tsr"
                     /locus_tag="KLK84_002173"
     CDS             complement(10110..11774)
                     /gene="tsr"
                     /locus_tag="KLK84_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HBE2009487.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11893..12339)
                     /gene="hpaR"
                     /locus_tag="KLK84_002174"
     CDS             complement(11893..12339)
                     /gene="hpaR"
                     /locus_tag="KLK84_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HBE2009488.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12610..13899
                     /locus_tag="KLK84_002175"
     CDS             12610..13899
                     /locus_tag="KLK84_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HBE2009489.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPAPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13896..15362
                     /gene="hpaE"
                     /locus_tag="KLK84_002176"
     CDS             13896..15362
                     /gene="hpaE"
                     /locus_tag="KLK84_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HBE2009490.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVSEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15364..16203
                     /gene="hpaD"
                     /locus_tag="KLK84_002177"
     CDS             15364..16203
                     /gene="hpaD"
                     /locus_tag="KLK84_002177"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HBE2009491.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRTI
                     LKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWREGQFK
                     EFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVNAVFP
                     LPA"
     gene            16213..16593
                     /locus_tag="KLK84_002178"
     CDS             16213..16593
                     /locus_tag="KLK84_002178"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HBE2009492.1"
                     /translation="MPHFIVECTENIREEARLPGLFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16661..17464
                     /gene="hpaH"
                     /locus_tag="KLK84_002179"
     CDS             16661..17464
                     /gene="hpaH"
                     /locus_tag="KLK84_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HBE2009493.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALMDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17475..18278
                     /gene="hpaI"
                     /locus_tag="KLK84_002180"
     CDS             17475..18278
                     /gene="hpaI"
                     /locus_tag="KLK84_002180"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HBE2009494.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPASIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18300..19655
                     /gene="hpaX"
                     /locus_tag="KLK84_002181"
     CDS             18300..19655
                     /gene="hpaX"
                     /locus_tag="KLK84_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HBE2009495.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19665..20555
                     /gene="hpaA"
                     /locus_tag="KLK84_002182"
     CDS             19665..20555
                     /gene="hpaA"
                     /locus_tag="KLK84_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HBE2009496.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTQAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDEKYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCPPTQFRGR"
     gene            20582..21519
                     /locus_tag="KLK84_002183"
                     /pseudo
     CDS             20582..21519
                     /locus_tag="KLK84_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21746..23304
                     /gene="hpaB"
                     /locus_tag="KLK84_002184"
                     /pseudo
     CDS             21746..23304
                     /gene="hpaB"
                     /locus_tag="KLK84_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23322..23834
                     /locus_tag="KLK84_002185"
     CDS             23322..23834
                     /locus_tag="KLK84_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HBE2009497.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            24227..26377
                     /gene="btsT"
                     /locus_tag="KLK84_002186"
     CDS             24227..26377
                     /gene="btsT"
                     /locus_tag="KLK84_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HBE2009498.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26427..26630
                     /locus_tag="KLK84_002187"
     CDS             26427..26630
                     /locus_tag="KLK84_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HBE2009499.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26641..27597
                     /gene="yjiA"
                     /locus_tag="KLK84_002188"
     CDS             26641..27597
                     /gene="yjiA"
                     /locus_tag="KLK84_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HBE2009500.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27666..>28425)
                     /locus_tag="KLK84_002189"
     CDS             complement(27666..>28425)
                     /locus_tag="KLK84_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBE2009501.1"
                     /translation="NYTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMART
                     EDFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQ
                     HADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQ
                     ALVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSF
                     YLTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6958 a         7429 c         7122 g         6916 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgctt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt
      601 gtatccagtg tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc
      661 tttcctcacg actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga
      721 acgccatagt gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc
      781 acgatcgcgg cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg
      841 agcatcatct gctggattac aaaggcgtag agaaacgatt tagccaaatt caaccattac
      901 gttacccaag atggctggta gccttaatgg ttggcctttc ttgcgcctgt ttctgtaaac
      961 tcaataacgg tggctgggat ggtgccgtca tcaccttctt tgccagtacg accgcgatgt
     1021 atatccgcca gctgctggca caacgtcatc ttcatccaca gatcaacttt tgccttaccg
     1081 ctttcgccgc caccaccatt tccggattgc ttttgcaact ccccactttc agcaataccc
     1141 ccaccattgc gatggccgcc agcgttctgc tgctagtgcc gggctttccg ttgattaatg
     1201 ccgtcgccga tatgtttaaa ggccacatca ataccggact ggcacgctgg gcgatcgcca
     1261 gtctgctgac actggctacc tgcgtcggcg tagtgatggc actgacgatt tgggggctac
     1321 gcggatgggt gtgatcgaat ttctgttagc gttggcgcag gatatgatcc tcgccgccat
     1381 tcctgcggtc ggctttgcga tggtgttcaa cgttcccgtg cgggcgttac gctggtgtgc
     1441 gctgcttggc tcgataggtc atggttcccg aatgatcttg atgaccagcg ggttgaatat
     1501 tgagtggtca acctttatgg cttctatgct ggtcggtacc attggtattc aatggtcgcg
     1561 ctggtatctg gcgcatccga aagtgtttac cgtggcggcc gttatcccta tgttcccggg
     1621 catatcggct tataccgcaa tgatttcgtc ggtaaaaatc agccagttag gttacagcga
     1681 accgttgatg attaccctgt taaccaactt tcttacagct tcatcgattg ttggtgcgtt
     1741 atccatcggt ctttccattc ctagattatg gttgtaccgc aagcgccctc gcgtataaaa
     1801 ttgcctcgct taagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1861 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1921 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1981 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     2041 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctagcgcgt ccgggaagcg
     2101 atgtcgctct ggacgaccaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca
     2161 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg
     2221 cgctatgggg cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg
     2281 cctactggca ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc
     2341 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2401 tcagtgaacc tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2461 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2521 gggtgaagag ctactggcct ggcagaaaga acaaggggcg atccgctccg ccgctctcga
     2581 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact
     2641 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2701 aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2761 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2821 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2881 cttcaggaat agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct
     2941 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     3001 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     3061 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa
     3121 cagtttatgg gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta
     3181 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     3241 aactattaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     3301 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3361 cagcacgaca caggaaggcg gttggtcact cgcgtcatta actaacttgc ttagcagcgg
     3421 aaaccaagcc ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc
     3481 gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3541 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggacg gtattcaggg
     3601 tttgctgaaa acaaaagatg gtcagcaact caatctggat aacatcggaa cgactccgct
     3661 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3721 ttcctgaacc gcgaaacgcc gcgttttagc tgttacccgg ctgacgcggc tctctcctgc
     3781 ccttttccct gctcctggac ggttttcccc ctgttacacg ggaagcgacc gcgatcaaaa
     3841 aagtctgttt ataaactaat tcctaacaac atcgcatttt tttgacacta gaattgcagc
     3901 aatatggcga cgccattaca ttgtgcttct caaaaagctt aaacgttggc taaacggccc
     3961 ggtttagtga ggatcgccca ttgtcagaac tactctcttt cgcccttttt ctcgcctctg
     4021 tgctgattta cgcatggaaa gcgggacgta acacctggtg gtttgcagcc acgttaacgg
     4081 tgctggggct atttgtcgtt ttaaatatca ccctgtttgc cagcgactat tttactggcg
     4141 atggtattaa cgacgcggtt ctctatacct taaccaacag cctgaccggt gctggcgtca
     4201 gcaaatacat cctgccgggt atcggcattg tgctggggct gacggcggtg ttcggtgcgc
     4261 tgggctggat cctgcgccgt cgtcgtcatc atccgcacca ttttggttac agcctgctgg
     4321 cgctcttact ggcgctgggt tcggtggacg ccagcccggc atttcgtcag ataacggaac
     4381 tggtgaaatc ccagtcacgc gatggcgacc cggactttgc ggcttattat aaagagccgt
     4441 cgaaaactat ccctgacccg aaactcaacc tggtttatat ctacggcgaa agtctcgagc
     4501 ggacctattt tgataacgaa gctttcccgg atctcacgcc tgaactgggc gcgttgaaaa
     4561 atgaaggcct ggatttcagc cacacgcagc agctgccagg aacggattac acgattgcgg
     4621 gcatggtggc ttctcagtgc ggcataccgc tgttcgcccc ctttgaaggc aacgcctccg
     4681 cctctgtctc cagcttcttc ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg
     4741 gttatcagaa ctatttcgtg cagggcgcaa atctgcgttt tgccggtaaa gatgtgttcc
     4801 tgaaatcgca cggcttcgac cacttatacg gctcagaaga gctgaaaagc gtggtagccg
     4861 acccgcacta tcgcaacgac tggggattct acgacgatac cgttctcgat gaagcgtgga
     4921 aaaagtttga agagctttcc cgctcaggtc agcgattctc actgtttacc ctgacagtcg
     4981 atacccatca cccggatggt tttatctcgc gcacctgtaa ccgcaaaaaa tatgattttg
     5041 acggtaagcc gaatcagtca ttcagcgcgg taagttgcag ccaggagaat atcgcgacgt
     5101 ttatcaacaa aatcaaagcg tcaccgtggt ttaaagatac tgtcatcgtc gtctcttctg
     5161 accatttagc gatgaacaac acggcgtgga aatacctcaa taagcaggat cgcaataacc
     5221 tgttttttgt cattcgtggc gacaagccgc agcaaaagac gctggcagtg aagcgtaaca
     5281 cgatggataa cggcgcgacg gtgctggaca ttctcggtgg cgataactat ctcggacttg
     5341 gtcgtagcag tttatccggg cagtcgatgt cggaaatctt cctcaatatc aaagagaaaa
     5401 cattggcgtg gaagccagat atcatccgcc tgtggaaatt ccctaaagag atgaaagagt
     5461 tcaccatcga ccagcagaaa aacatgattg ccttctcggg tagccatttc cgtttgccgc
     5521 tgctgttgcg ggtttcagac aaacgcgtgg aaccgctgcc ggaaagcgaa tactcagcac
     5581 cgctgcgttt ccagctggcc gatttcgctc cacgcgacaa tttcgtctgg gttgacagtt
     5641 gctacaagat ggcacaactc tgggctccgg aactggcact ttccaccgac tggtgtgtct
     5701 cgcaggggca gcttggcggt cagcaaattg ttcagcatgt tgacaaaaca acatggaaga
     5761 gcaagacggc atttaaagat acggtcatcg acatggcgcg ttacaaaggc aatgtcgata
     5821 cgctgaagat tgttgataac gatattcgct acaaagccga cagtttcatc tttaacgtcg
     5881 ccggtgcgcc agaagaggtg aaacagttta gcgggatttc ccgtccggag tcgtggggcc
     5941 gctggtccaa cgcgcagctg ggcgatgaag taaaaatcga gtacaagcat ccgctgccga
     6001 agaaatttga cctggtgatt accgccaaag catacggcaa taacgccagc cgtcctattc
     6061 cggtacgcgt aggcaatgaa gaacaaactc ttgtgctggg caatgaagtg accaccacca
     6121 cactgcattt cgataaccca accgatgccg acacactggt aattgtgccg ccggaacctg
     6181 tctcaaccaa cgaagggaat atcctcggac actcgccgcg taagctcggg atcggcatgg
     6241 tggaaattaa agtggtagaa cgtgaagggt aattcctgac tgatgtgtag gtcgcatagg
     6301 gtgcgaccta cacgccttct ccggcttaca acacattatt aagaaacaaa aaaaacagcg
     6361 cccaacactt ccccggacgc tgcatggcaa ctttaataat ctcgccttat cagaaagtaa
     6421 ttacaccctt aatcaactca cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg
     6481 tggcgaacgg atagcgatgg gttaacatca tgtcggcagt gattttccct tccgccatca
     6541 gtcgaccgac tttagcgaaa tcttccggcg tggcgttgcg gctgcccatc atcgtcgttt
     6601 cttttttatg gaattccgga tcggagaact gcaactcacc tttaaacaga ccgacaaata
     6661 ccaccgtgcc gccgtgacga atcagattca cggtattatt catcgcatgt tgattacctg
     6721 tcgcgtcgat cactttctgc gccagcgaac caccaaactg cgcccgcagc tgcgcgtcaa
     6781 aatcttcggc taacgggtcc agcacgggca actccagacg cgttgccaca tgttcacggc
     6841 gcgccggact ggtatccgcc accaccacct gtgcgccatc ggctttagcg attgccgccg
     6901 cgcccagacc gattggcccc gccccgacca ccagcacctg ctcgccggga gcaatggctg
     6961 cgcgacgcac cgcatgagcg ctaatagcga aaggttcaat caatgcggcc gcctgcgggt
     7021 caataccgtc tgcgggcaaa atgttcgcca ccggcacgct caaatactca ctaaaaccgc
     7081 catcctgatg cacgccaatg actgaaattt tttcacagca gttggtacgc ccgcttttac
     7141 acgccgggca ttgctgacag gcaacataag ggatcacagc aacttgctga ccatttttaa
     7201 gatcagcaat atttttaccc agcccaacaa tctccccaca tatttcatgg cctaaaaccc
     7261 gtggataact aaaaaatggt tgatttccac cctaggcatg aatatcggta ccgcaaatcc
     7321 cgacagactt aatttttatt aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt
     7381 tccagactaa ttctttcggc tgctggcaaa ttaaaacatt catcgtagac atgattcggc
     7441 tatccatttt ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa
     7501 tctgttttgt gaaaacacac gcataaaaat gttttaaatc gggttttaat ggactcacaa
     7561 ggaggaaaat atgagtcgtt cacaaaattt acgccacaat gtgattaacc aggtcattga
     7621 tgatatggcg cggggtcata tcccttcccc acttccatcg caaagtgcgc tggcggaaat
     7681 gtacaacatc agccgcacta cagtgcgtca tattctcagc caattacgcg aatgcggcgt
     7741 cttaacgcag gttggaaacg actatgttat tgcgcgtaaa cctgaccatg atgacggttt
     7801 cgcctgtact accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg cttttttcac
     7861 gatgataaac cagcgtcagt tacgccctag ggaaactttt tctgaactgc aactggctcg
     7921 ggccgcgggt gtcagcccgg tggttgtaag agaatatctt ttaaaattcg gccgttataa
     7981 tcttattcaa agcgaaaagc gcggccagtg gagcatgaag caattcgatc aatcctacgc
     8041 cgagcagctg tttgagctac gcgaaatgct cgaaacacat tcacttcagc actttctcaa
     8101 cctgcctgat catgatcccc gctggctgca ggcgaaaacc atgctagaac gtcatcggct
     8161 actgcgtgac aacatcggca atagctttcg catgttctcg caacttgaca gagattttca
     8221 ctccttactg ctttcagctg ccgacaatat cttttttgat caatcgcttg agatcatctc
     8281 agtcatcttt cattttcact atcaatggga cgaaagcgat cttaaacagc gcaatatcat
     8341 tgccgtcgat gaacatatga ccattctcag cgctctgatt tgccgcagtg accttgatgc
     8401 cacccttgcg ctccgtaacc atttgaattc agctaaacaa tcaatgattc gatccattaa
     8461 tgagaacacg cgttatgctc attaagtacg gattaaaaac agcaaactat tagcgggtgc
     8521 acggttttat caaaagataa ataccgggaa attcccgctt acctatgctc acaatcagtc
     8581 gactaaaaat gtttttgtga gcggtagtaa agtcctaaaa ctttaacctg ccgttaatgt
     8641 caaccttgcg taatgcacaa ttacgtaaac aattaaaatt ataaatacag gagtccggcg
     8701 tggaaaaaga aaatatcacc ctcgatccgc gttcttcatt tactccatct tcgtcggcag
     8761 atattcccgt gccaccagat ggattagttc aacgcagtac ccgaattaaa cgcattcaaa
     8821 ccaccgccat gttgttatta ttttttgcgg cggtaatcaa ttatctcgac cgcagttcgc
     8881 tgtcggtagc aaatttaacg attcgtgaag aattgggatt aagtgccacc gaaatcggcg
     8941 ctttgctctc cgtgttttca ctcgcttacg gcattgcgca acttccttgc ggcccactat
     9001 tggatcgtaa aggcccacgc ctgatgctgg gactggggat gttcttctgg tcactgttcc
     9061 aggccatgtc tggcatggtg cacagcttta cgcagttcgt gttggtgcgt atcggtatgg
     9121 ggattggtga agcgccgatg aacccatgcg gtgtaaaagt cattaacgac tggttcaaca
     9181 tcaaagagcg cggacgcccg atgggcttct tcaacgcagc ttctaccatt ggcgttgctg
     9241 taagcccacc gattctggcg gcaatgatgc tggtgatggg ctggcgcggg atgtttatta
     9301 ccattggtgt actggggatt tttctcgcca tcggctggta tatgctctat cgcaaccgcg
     9361 agcacgtaga actgactgcc gttgaacaag cttatctcaa tgcaggtagc gtcaatgccc
     9421 gccgagatcc gctcaatttt gccgaatggc gcagcctgtt ccgtaaccgc acaatgtggg
     9481 gaatgatgct cggattcagt ggcatcaact acactgcgtg gctgtatctg gcctggcttc
     9541 ctggttacct gcaaacagcc tataacctgg atttaaaaag cacagggttg atggcggcta
     9601 tccctttcct gtttggggct gccgggatgc tggtcaacgg ttacgttact gactggctgg
     9661 tcaaaggggg aatggctccg attaaaagcc gtaagatctg cattattacc gggatgttct
     9721 gttctgccgc ctttacgctg atagtaccac aagcgacaac atccatgatg gcggttctgc
     9781 tgattggcat ggcactgttc tgtattcact ttgccggaac atcctgctgg ggcttgatcc
     9841 acgtcgcagt tgcttctcgc atgactgcgt cggtgggcag tatccagaac tttgccagct
     9901 tcatctgcgc ctctttcgcg ccgatcatta ctggttttat tgttgataca acccactcat
     9961 tccgtctggc actaatcatt tgcggttgcg tcaccgcagc gggagcactg gcgtacatct
    10021 tcctggttcg ccagccgatc aacgacccgc gtaaagatta aactccctat gcccgataac
    10081 attttgctta tcgggcattt ttatggcgat taaaacgttt cccagttctc cccgctatct
    10141 gccactgcca ttttacgcgg cgtagctggc gtcacggttt ttaccacagc cgatgtttca
    10201 cgctgctgtt gctgctgttg ctgaatccgg aacactgcca cagcttcggt caggcgactg
    10261 gcctgctctt ccagcgcggc ggcggcagcg gcagattctt ccaccagcgc ggcgttctgt
    10321 tgagttaccc ggtccatctc agcaaccgct aagccaacct gatcgatacc acggctctgc
    10381 tcatcagaag cagaagctat ttcgcccata atgtccgtca cgcgggtcac agcactgaca
    10441 atctccgcca ttgtttcccc ggcgctttcg accagcgtag agccaacatc cactttcccc
    10501 accgagtctt caatcaggct tttaatttca cgaaccgcct gggcgctacg ctgggccaga
    10561 ttacgcactt ctcccgcaac caccgcaaaa ccacgtcctt gctcacccgc acgcgctgct
    10621 tcaaccgccg cgttcaaagc cagaatattg gtctggaagg taatgccgtc aattacgctg
    10681 ataatatcgg cgattttctg cgaactggtg gagatatcgc gcatagtctg caccacgtta
    10741 tctaccactt tgccgccgcg ttgcgccgtt tcagaagcac ttaatgccag atggctggcc
    10801 tggcgcgcat tctcggcgtt ctgtttcacc gttgcggtca gttgctccat gctggctgcc
    10861 gtctcttcca gcgaagcggc ctgttgctcg gtgcgcgaag agagatcatt attgccggta
    10921 gcgatttcgc tggcaccgct atagatggca ttggccccgt tgcgcacatc accgacggta
    10981 cgcatcagct ctccctgcat atggcgcaaa ctctctgcca gttgccccat ctcattagag
    11041 ccatccacct caatcggttt caccagatcg ccgcctgcaa tatgacgaat gctgtcaatc
    11101 aggcgattca ttggcgctac cagcgaggct ttaataccga accagacggc gaagatgacc
    11161 gccagtacga cgatcatcac gcccaccaga atccacatcg cctggctgta ggaggcattg
    11221 ttatcgctga cggcgatatc atagagccga tcgttttgct ccatgtaagc cacatactgc
    11281 ttctcgaaac cgtcctgata tccctgggtc ggctgatcaa agaactcgtt gattttgcct
    11341 gcacctaaca gttggatcag ctccgccagc gcattgtgat aaatatcgta attacgtttg
    11401 atctctgccg ctgcggcggt gctctgacgc gggtcacgcg gcaacgcttc gtaatccgcc
    11461 cagttttttt ccgcctgttt cagcgaaata ctggcactct gcatcagctc agcaacggtt
    11521 gaaccgctac caatattatt ctgatccatc atgtagcgga tacccgcgcg gttgagggtg
    11581 ttacgcgtct gcaacaacgc gacccagctg ccattcagcg tgggttgctg ctggcgaatg
    11641 gtttgtaaaa cagtgaaatt ttctttgtca ttctttaagg cattaaaaaa cagaccgcct
    11701 gatgtcagtt gtaaaaggcc aaaaacggcc aaaaccagca gtaagctggt cacgatcttg
    11761 atacgtttta acatggtttc tctttcctgt ggaccgatgc aagattttcg gcctggaaag
    11821 caaaaacttt atgaaaaggt gtaacccgcc atccatggaa aaaccaggcg ctggttatca
    11881 gatactaaaa agttattcat tatcgcccgg gatgtcttct tgtcgggaat tgcccagagc
    11941 aataaattcc tctaacagat gggttaactg ttgcattttc tcggcagtaa actgagcttc
    12001 aatctgccga taggcttctt caatctgtgt ctgagcgcgg ttatacagcg cctgcccctc
    12061 tttggtcagc gagatataga gcttccgctg atcattgatc ggctttaatc gcaacactaa
    12121 accgtcgcgt tccatccgcg tgaggatccc ggtcaggctt gggcgcaaaa tgcaggcgcg
    12181 atacgccaga tcgtgaaaat ccattgatgg gctttccgcc agaatacgca cgatgcgcca
    12241 ctgttgctca gtcaaattat gccgtttaac gattgggcga aaataactca tcgccgcttc
    12301 tcgcgcctgg agcaacgcaa tggttagtga gtcgtgcatt atctttcccc tgaaaaattc
    12361 aaaatcatta atatagaaac aattaattat cacgttaccg ggactgagct gccccgcaat
    12421 tcttcatttt atcaaaattg atgatatttt attaacttac tgatatttaa tattttatta
    12481 tctttaaatg taaatagttt gttaattaga tcacatttat atcacttact tttgccaaac
    12541 tacgcaatcg ggaataaaag taatcattaa catattaatg attaagatga tcgaatccga
    12601 ggagtggtaa tgaaaggcac tatcttcgcc gtagcgttga accatcgcag ccaacttgat
    12661 gcctggcagg aagcgttcca gcaatctccc tacaaagccc cgcctaaaac tgcggtctgg
    12721 tttattaaac cgcgcaatac ggtgattggt tgcggtgaac cgattccctt tccacagggt
    12781 gaaaaagtac tgagcggtgc gactgttgcg ctgattgtgg ggaaaacggc gacgaaagta
    12841 cgtgaagaag atgcggcaga gtacatcgcc ggatatgcgc tggctaacga cgtcagcctg
    12901 ccggaagaga gcttttaccg tccggcaatc aaagcaaaat gtcgtgatgg attctgcccc
    12961 attggcgaaa ccgtggctct cagcaatgtc gataatctga ctatctatac cgagatcaac
    13021 gggcgtcctg ccgatcactg gaataccgcc gatttacaac gtaacgccgc acagctgctg
    13081 agcgccctga gcgaatttgc cacgctgaat ccaggcgatg ctattctgct cggcacgcca
    13141 caggcgcgcg tggaaataca gccaagcgat cgcgtgcgtg ttctcgcaga aggtttcccg
    13201 ccgctggaaa atccggtagt ggacgaacgt gaagtgacca cgcgcaagag cttcccaacg
    13261 ctgccacacc cgcacggtac gctgtttgcc ctcggcctga actacgccga ccacgccagc
    13321 gaactggaat ttaagccacc ggaagagccg ctggtgttcc tgaaagcgcc gaataccctc
    13381 actggcgaca accagacctc cgtgcgcccg aacaatattg aatacatgca ctacgaagcg
    13441 gagctggtgg tagttattgg caagcaggcg cgtaacgtca gcgaagccga tgccatggat
    13501 tatgtcgcgg gctacaccgt gtgtaacgac tacgccattc gcgactatct ggaaaactac
    13561 taccgcccta acctgcgggt caaaagccgc gacggactga cgccgatgct ttcaaccatc
    13621 gtgccgaaag aggcgatccc ggccccgcat aatctgaccc ttcgcacctt cgtcaacggc
    13681 gagttacgcc agcaaggcac caccgccgat ctgatcttca gcgtgccctt cctgatcgcc
    13741 tacttaagcg aatttatgac cctgaatccg ggcgacatga tcgccaccgg cacaccaaaa
    13801 ggcttatctg acgtggtgcc tggcgatgaa gtagtggtgg aagtagaagg cgtgggccgc
    13861 ctggtgaacc gaattgtgag tgaggaaaca gcgaaatgaa aaaagtaaat cattggatca
    13921 acggcaaaaa tgttgcaggt aacgactact tccagactac caatccggca acgggtgaag
    13981 tgctggcgga tgtggcctct ggcggtgaag cggagatcaa tcaggcggta gcggcagcga
    14041 aagaggcgtt cccgaaatgg gctgatctgc cgatgaaaga gcgtgcgcgc ctgatgcgcc
    14101 gtctgggcga tctgatcgac cagaacgtgt ctgagatcgc cgcgatggaa accgcggaca
    14161 cgggcctgcc gatccatcag accaaaaatg tgttgatccc acgcgcttct cacaactttg
    14221 aatttttcgc ggaagtctgc cagcagatga acggcaagac ctatccggtt gacgacaaga
    14281 tgctcaacta cacgctggtg caaccggtag gcgtttgtgc gctggcgtcg ccgtggaacg
    14341 tgccgtttat gaccgccacc tggaaggtcg cgccgtgtct ggcgctgggc aataccgcgg
    14401 tgctgaagat gtcggaactc tccccgctga ccgccgaccg cctgggtgag ctggcgctgg
    14461 aagctggtat tccggcgggc gtgctaaacg tggtacaggg ctacggcgca accgcaggcg
    14521 atgcgctggt ccgtcatcat gacgtgcgtg ccgtgtcgtt caccggcggt accgccaccg
    14581 ggcgcaacat catgaaaaac gccgggctga aaaaatactc catggagctg ggcggtaaat
    14641 caccggtgct gatttttgaa gatgccgata ttgagcgtgc gctggacgcc gccctgttca
    14701 ccatcttctc aatcaacggc gaacgctgca ccgccggttc gcgcatcttt attcagcaga
    14761 gcatctatcc agaattcgtt aaacgctttg ccgaacgcgc caaccgtctg cgcgtgggcg
    14821 atccgaacga tccgaatacc caggttaggg cgcttatcag tcagcaacac tgggaaaaag
    14881 tctccggcta tatccgtctc ggcattgaag aaggcgcaac cctgttggcg ggcggcccgg
    14941 ataaaccgtc cgacctgcct gcgcacctga aaggcggcaa cttcctgcgc ccaacggtgc
    15001 tggcggacgt agataaccgt atgcgcgttg cccaggaaga gattttcggg ccggtcgcct
    15061 gcctgctgcc gtttaaagac gaagccgaag gcttacgcct ggcaaacgac gtggagtacg
    15121 gcctcgcgtc gtacatctgg acacaggatg tcagcaaagt gttacgcctg gcgcgtggca
    15181 ttgaagcagg catggtgttc gtcaacaccc agaacgtgcg cgacctgcgc cagccatttg
    15241 gcggcgtaaa agcctccggc accgggcgtg aaggcggtga gtacagcttc gaagtgttcg
    15301 cggaaatgaa gaacgtctgc atttccatgg gcgaccatcc aattccgaaa tggggagtct
    15361 gatatgggta agttagcgtt agccgccaag atcacgcacg taccgtcaat gtatctctct
    15421 gaactaccag gaaaaaacca cggttgtcgc cagggtgcga tcgacgggca taaagagatc
    15481 agcaagcgtt gccgggaaat gggcgtcgat accattatcg ttttcgatac tcactggctg
    15541 gtcaacagtg cttatcacat caactgtgca gaccattttg aaggcgtcta caccagcaac
    15601 gagctgccgc attttattcg cgacatgacc tacaactacg agggcaaccc ggagttgggg
    15661 cagctaattg ccgaggaagc cttaaagctc ggcgtgcggg caaaagcgca caatattccc
    15721 agcctgaaac tggaatacgg cacgctggta ccgatgcgct acatgaatga agacaagcac
    15781 ttcaaagtgg tctccatttc ggctttctgc acggttcacg attttgccga cagccgcacg
    15841 attctgaaag cgatcgaaca gtacgacggc accgtggcgg tccttgccag cggttcgtta
    15901 tcgcaccgct ttattgacga tcagcgtgcg gaagaaggga tgaacagcta cacccgcgag
    15961 ttcgaccgcc agatggacga gcgtgtggtg aagctgtggc gcgaaggcca gttcaaagag
    16021 ttttgcaata tgctgccgga gtacgccgac tactgctacg gcgaaggcaa tatgcacgac
    16081 acggtgatgc tgctggggat gctcggctgg gataaatacg acggcaaggt ggagtttatt
    16141 accgagctgt tcccaagctc tggcaccggt caggttaacg ctgttttccc gctgcccgcg
    16201 taaggacgtt ttatgccgca ctttatcgtg gaatgtactg aaaatattcg tgaagaagcc
    16261 cgcttacctg ggctgtttgc cagtgttaac actgcactgg cggcaacggg gatttttccg
    16321 cttgggggaa tacgtagccg ggcgcactgg atcgatacct ggcaaatggc tgatggcaag
    16381 cacgattacg cctttgtgca tatgacgcta aaaatcggca gcggacgcag tctggaaagc
    16441 cgtcaggaag tgggcgaaat gctgtttgat ctgattaaaa cgcacttcgc ctcactgatg
    16501 gagagccgtt acctagcgct ttcatttgag attgcagagc tgcacccgac gttgaatttc
    16561 aaacagaaca acgtacacgc gttgtttaaa taaatttgca ccagcttcca ggccggatga
    16621 taaggcgctg ccgcatccgg caatgccaca ggatatcgct atgttcgata aacacaccca
    16681 caccctgatc gcccagcgtc tggatcaggc agaaaaacag cgcgaacaga tccgcgcgat
    16741 ctcgctggat tacccggaga tcaccatcga agacgcctac gccgtgcagc gcgaatgggt
    16801 tcgcctgaaa atcgccgaaa gtcgcacgct gaaaggccac aaaatcggtc tgacctcgaa
    16861 agcgatgcag gcaagctcgc agatcagcga accggattac ggtgcactga tggacgacat
    16921 gttcttccac gatggcagcg atatcccgac cgatcgcttt atcgtgccgc gcattgaagt
    16981 ggagctggct tttgtgctgg caaaaccgct gcgtgggcca aactgcacgc tgttcgacgt
    17041 ttacaacgcc acggactatg tgatcccggc gctggagctg atcgacgctc gctgccacaa
    17101 catcgatccg gaaacccagc gcccgcgcaa agtattcgac accatttccg ataacgccgc
    17161 caacgccgga gtgatcctcg gaggtcgtcc gatcaaacca gacgaactgg atctgcgttg
    17221 gatctccgcc ctgatgtatc gcaatggcgt gattgaagaa accggcgtcg ccgctggtgt
    17281 gctgaatcat ccggcaaacg gcgtggcctg gctggcgaac aaactcgcac cgtatgacgt
    17341 acaactggaa gccgggcaaa tcattctcgg tggttcgttc acccgcccgg ttccggcgcg
    17401 taagggcgac accttccacg tcgattacgg caacatgggc tccattagct gccgctttgt
    17461 ttaaggagag aacgatggaa aacagtttta aagcggcgct gaaagcaggc cgtccgcaga
    17521 ttggattatg gctggggctg agcagcagct acagcgcgga gttactggcc ggaccaggat
    17581 tcgactggtt attgatcgac ggtgagcacg cgccgaataa cgtgcaaacc gtgctcaccc
    17641 aactacaggc gattgcgccc taccccagcc agccagtggt acgcccatcg tggaacgatc
    17701 cggtgcaaat caaacaactg ctggacgtag gcacacaaac cttactggtg ccgatggtac
    17761 aaaacgccga cgaagcccgt aaagcggtac gcgccacccg ttatcccccc gccagtattc
    17821 gcggtgtggg cagtgcgctg gctcgcgcct cgcgctggaa tcgcattcct gattacctgc
    17881 aaaaagccaa cgatcaaatg tgcgtgctgg tgcagatcga aacgcgtgag gcaatgaaga
    17941 acttaccgca gattctggac gtggaagggg tcgacggcgt gtttatcggc ccggcggatc
    18001 tgagcgccga tatgggttat gccggtaatc cgcagcaccc ggaagtacag gccgccattg
    18061 agcaggcgat cgtgcagatc cgcgaagcgg gcaaagcgcc ggggatcctg atcgccaatg
    18121 agcaactggc aaaacgctat ctggaactgg gcgcgctgtt tgtcgccgtc ggcgttgaca
    18181 ccaccctgct cgcccgcgcc gccgaagcgc tggcagcacg gtttggtaat agttcttcta
    18241 tttcgatggc gaagcaaaac aacaactctg tctactaaac gtacgatgtg gagcgcatca
    18301 tgacgacctc taccctgcaa tataaagata ataaaactgt tgaaattgaa catcgtgtca
    18361 ttaataaatt atttcgtcgc ctaatcattt ttctttttat tctgtttgtt ttttcgtttc
    18421 ttgatcgcat caatatagga tttgccggac tgacaatggg caaggatctt ggcctgacgt
    18481 ccacaatgtt tggcctcgcc gcaacactct tttatgtcac gtacgtcatg tgcgggatcc
    18541 ccagtaatat gatgttagcg aaaattggcg ctcgtcgatg gatcgctggg atcatggtgg
    18601 tctggggaat tgcttccacc tgcacaatgt ttgcaacaag ccccgaaaca ctctatctac
    18661 tgcgtatgct ggttggtatt gctgaggcgg gttttttacc tggtattttg gtttatctga
    18721 cctggtggtt cccggcatat catcgggcca gagccaatgc cttatttatg atagcgatgc
    18781 cagtcactat gatgctggga ttgatccttt caggctacat tctggccttg gatggactgt
    18841 ggaatctaaa aggctggcaa tggctctttt tgctggaagg tctgccatcg gtcattcttg
    18901 gtgtcgtcac ctggttttat ctcaacgaca caccagacca ggcgacctgg cttgatgaaa
    18961 acgaaaaaca ggctttaaaa acgatgattc tccgtgaaca agagagtgca tttgcccatg
    19021 catctgttcc acgctcaacc ttacgtgaag tcctgacccc agccatcatg ctatatacac
    19081 ttgcttattt ttgcctgact aatactctga gtgcgattaa tatctggacg ccgcaaatcc
    19141 tacagagttt caatactggc agtagcaaca tcatgattgg tttgctggcg gccatccccc
    19201 aattctgcac aatttttggc atgatctggt ggagtcgtcg ttccgaccgt cttaaagaac
    19261 gcaaaaagca tacgattctt ccctacctgt ttgccgctgc cggatggtta ctcgcttctg
    19321 cgacagatca cagcctcatt caactgagca gcatcattat ggcctcaatg ggatcattta
    19381 ccgcgatgac tatcttttgg acaacaccgg atcaagttat aagcttgcaa tcgcgtgccg
    19441 ttgcgatggc tgtcatcaat gctgtcggca atgtaggttc agcagtcagt ccattactta
    19501 tcggtatttt gaaagatgca accggcagtt ttagctcagg tttgtggttt gtagcaggat
    19561 tgctggtact gggcgcgtta gtcatgaccc gaatccctat ggggagtcgg gaacactctc
    19621 tttcatctga acttatcgca ccaaaaccgc aataaggagt catgatgtcc agtgaagcta
    19681 tctttgaaca tggttatgaa aatattgatt tggtccggga atatgatgcc cgctacgctg
    19741 gtgaagatgt gcattatgaa acatttgccc ggttagcgaa tttctttgga cgcgatatgc
    19801 gccctcactg gcacgataga tattttcaac tccattatct ggtcactgga cgcattactt
    19861 tacaactgga tgagcacttc tattctgtgc gcgcaccgct ttttgtacta acaccaccat
    19921 cggtgccaca tactttcttt actgatcctg atagcgatgg gcatgttctg accgtacgtc
    19981 aggagttaat ttggcccctg gtagaaaagc tctggcctgg tagtggagat gccgcaatgc
    20041 tacaaggtat ctgtctgtca ctggagggaa tggaacatac acaggcggca ttaaatcatt
    20101 attggccgat aatatccctg gagtttcaac aaaataagca aggacgggaa attctattgc
    20161 aatcactggc gcaaagtatt tttacaatcc tactgcgtga agcaccacca aatgatattt
    20221 caacatgcag cgtgcgcggc gagatgtgtt tattccagaa gtttaatcgg ctgattgacg
    20281 aaaaatatcg ccaacattta atggtccctg aatatgcaac aatgctcggt atgagtgaat
    20341 cacggcttac cgagctgtgc cgacgttttg ccaatcaatc gccgaagaga ttgattttcg
    20401 aacgagtgag ccgggaagcc agacggcttc tgctctatag cgagcaaagc atcaaccaga
    20461 tagcccttga tcttggctac aaagatcccg cctatttcgc ccgttttttc aaccgaatga
    20521 tgggttgccc accaacacaa ttccgtggtc gctgaccatt tatcaacctg agagactcga
    20581 catgaaaata tctttacgct cactatttgt ggcgtgcctg attgcgccag tggcattcac
    20641 ctacgcctcc gaaccgcaat aaatgaaaat tgtactactt ggaacgaaag gtggcccttc
    20701 tttattaaaa gctgaccgtt taccgatggc aacggcactt attattggca ataaaaccta
    20761 gttaattgat gctggttacg gggccagctt acaactggta aaaaataata ttccactgcg
    20821 ccagattgat aatattttac tcacccattt acacagtgat catattctgg actatccatc
    20881 agtcttaatg aatgcatggg ctagcggtct gcaggacaaa actattcatg tattcggtcc
    20941 gccaggcacc cgagccatga ccgctgccag ttggcaggtc ttcgatcggg acatcacact
    21001 gcgtatggaa gaagaaggaa aacccgatcc tcgcaaactc gttacagctc aagatgttgg
    21061 tgaaggcgta gtttataaag atgataccgt gaccgtgact gcattaaagg tccctcattc
    21121 tcctttccct gacggtgagg cttttgctta tcgcttcgac acacaaggca agcgaattgt
    21181 cttctcagga gatacctcgt actttccccc actggcaact ttctcagaag gcgctgatat
    21241 acttgtgcat gaggccgtac atgagccctc cgtcgctaag ctggcagaca gtattggcaa
    21301 tggtaaaaca ctggcaaaag ccattgcttc tcaccatacg actattgagg atgtaggcaa
    21361 aattgccaat gaagcaaaag tgaaaaaact catccttagc cacctggtac caatcaccgt
    21421 tccggatgaa acatggaagc aagaagcaca gaaaactatt caggccctat cgtcgttggt
    21481 catgaaaata tgacgattac cattccattc gccccataaa aaattccgtc cccgttagcc
    21541 cttgttagcg gggaatatta tgatttgcct tactgtaagt tgatcacgat cgcagtttac
    21601 tatttaactc caccttgctg tcgaaaagta ccaacgttcg cctgaaacgt ctcttcccga
    21661 caaccgcgtt caacgcttca attaaataac aaaaacaaca cacaaaatta acaatcgtat
    21721 tccgattaat actgtagagg tcgacatgaa accagaagat ttccgcgcca gtacccaacg
    21781 tcctttcacc ggggaagagt atctgaaaag cctgcaggat ggtcgcgaga tctatatcta
    21841 tggcgagcga gtgaaagacg tcaccactca tccggcattt cgtaatgcgg cagcgtctgt
    21901 tgcccaactg tacgacgcgc tgcacaaacc ggagatgcag gactctctgt gttggaacac
    21961 cgacaccggc agcggcggct atacccataa attcttccgc gtggcgaaaa gtgccgacga
    22021 cctgcgccag caacgcgacg ccatcgctga atggtcacgc ctgagctatg gctggatggg
    22081 ccgtacccca gactacaaag ccgctttcgg ttgcgcactg ggcgcgaatc cgggctttta
    22141 cggtcagttc gagcagaacg cccgtaactg gtatacccgt attcaggaaa ctggcctcta
    22201 ctttaaccac gcgattgtta acccaccgat cgtcatttgc cgaccgataa agtgaaagac
    22261 gtttacatca agctggaaaa agagactgac gccgggatta tcgtcagcgg tgcgaaagtg
    22321 gttgccacca actcggcgct gactcactac aacatgattg gcttcggctc ggcacaagtg
    22381 atgggcgaaa acccggactt cgcactgatg ttcgttgcgc caatggatgc cgatggcgtg
    22441 aaattaatct cccgcgcctc ttatgagatg gtcgcgggtg ctaccggctc gccatacgac
    22501 tacccgctct ccagccgctt cgatgagaac gatgcgattc tggtgatgga taacgtactg
    22561 atcccatggg aaaacgtgct gatctaccgc gattttgatc gctgccgtcg ctggacgatg
    22621 gaaggcggtt tcgcccgtat gtatccgctg caagcctgtg tgcgcctggc agtgaaactc
    22681 gacttcatta cggcactgct gaaaaaatca ctcgaatgta ccggcaccct ggagttccgt
    22741 ggtgtgcagg ccgatctcgg tgaagtggtg gcgtggcgca acaccttctg ggcattgagt
    22801 gactcgatgt gttcagaagc gacgccgtgg gtcaacgggg cttatttacc ggatcatgcc
    22861 gcactgcaaa cctatcgcgt actggcacca atggcctacg cgaagatcaa aaacattata
    22921 gaacgcaacg ttaccagtgg cctgatctac ctcccttcca gtgcccgtga cctgaacaat
    22981 ccgcagatcg accagtatct ggcgaagtat gtgcgcggtt cgaacggtat ggatcacgtc
    23041 cagcgcatca agatcctcaa actgatgtgg gacgccattg gcagcgaatt tggtggtcgt
    23101 cacgagctgt atgaaatcaa ctactccggt agccaggatg agattcgcct gcaatgtctg
    23161 cgccaggcac aaagctccgg caatatggac aagatgatgg cgatggttga tcgctgcctg
    23221 tcggaatacg accagaacgg ctggactgtg ccgcacctgc acaacaacga cgatatcaac
    23281 atgctggata agctgctgaa ataacgcagc aggaggttaa gatgcaatta gatgaacaac
    23341 gcctgcgctt tcatgacgcg atggccagcc tgtcggcagc ggtaaatatt atcaccaccg
    23401 agggcgacgc cggacaatgc gggattacgg caacggctgt ctgctcggtc acggatacac
    23461 caccgtcgct gatggtgtgc attaacgcca acagtgcgat gaacccggtt tttcagggca
    23521 acggcaagtt gtgcgtcaac gtcctcaacc atgagcagga actgatggcg cgccacttcg
    23581 cgggcatgac aggcatggcg atggaagagc gttttagcct ctcatgctgg caaaaaggtc
    23641 cgctggcgca gccggtgcta aaaggttcgc tggccagtct tgaaggagag atccgcgatg
    23701 tgcaggcgat tggcacacat ctggtgtatc tggtggagat taaaaacatc atcctcagtg
    23761 cagaaggtca cggacttatt tactttaaac gccgtttcca tccggtgatg ctggaaatgg
    23821 aagctgcgat ttaagctcca gccccttcac gcattcgtcg aaggggcatt tttattgtga
    23881 tctcaatcac atatctaccc gatgaataat tcttgcccaa ccgctcaccg ccattcctga
    23941 ccgcttacat ccctaaaata accactcagt tatttacctt actttacgcg cacgtatctc
    24001 tggcaacatc actgcaggat agcggtcaat ttacctcctc aaacgcaacg caaacctaga
    24061 acggcttcgg cctatgatta acaaattact ctatggattt tggtttgcag gaaggcagca
    24121 agtgagtgaa tccccgggag cttacaaaag taagtgtcag aggtgaacga acgcagctgc
    24181 cgcacctgta agccaaaaga cgacgagtaa aatgccaggt tttactatgg atacgaaaaa
    24241 actattcaag cacataccct gggtgattct cggaatcatc ggtgcattct gcctcgcggt
    24301 agttgcatta cgtcgggggg agcacgtcag cgccctgtgg atcgtggtcg cctctgtgtc
    24361 ggtgtatctg gtggcgtatc gctactacag tctgtacatc gcccagaagg tgatgaaact
    24421 cgaccccacg cgcgcgacgc ctgcggttat taacaacgac ggtctgaact acgttccgac
    24481 caaccgttac gtgttgtttg gtcaccactt cgccgctatc gccggtgctg gtccgctggt
    24541 gggaccggtt ctcgccgcgc agatgggcta cctgcctggc acgctgtggc tgctggcggg
    24601 ggtagtgctg gccggtgcgg tacaggactt tatggtgctg tttatctcct ctcgccgtaa
    24661 cggcgcatct ctcggtgaga tgatcaaaga agagatgggg ccagtaccgg ggactatcgc
    24721 gctgtttggc tgtttcttaa tcatgatcat catcctcgcc gttctggcgc tgattgtggt
    24781 taaagccctg gccgaaagtc cgtggggtgt cttcaccgtt tgctcaaccg taccgattgc
    24841 gctgtttatg ggtatctaca tgcgctttat ccgtccgggg cgtgtgggtg aagtctctgt
    24901 tattggtatc gtgctgctgg ttgcctctat ctacttcggt ggcgtgattg ctcacgatcc
    24961 gtactggggt ccggcactga cctttaaaga caccaccatt accttcgcgc tgattggcta
    25021 tgcgtttgtt tccgcactgc tgccggtgtg gctgatcctc gcaccgcgcg actatctggc
    25081 aaccttcctg aaaatcggcg ttatcgtcgg cctggcgctg ggtatcgtgg tgctgaaccc
    25141 ggaactgaaa atgcctgcga tgacccagta cattgacggt actggcccgc tgtggaaagg
    25201 cgctctgttc ccgttcctgt tcatcaccat cgcctgtggt gcggtatctg gcttccacgc
    25261 gctgatctct tccggtacga cgccgaaact gctggctaac gaaaccgacg cgcgtttcat
    25321 tggctacggc gcaatgctga tggagtcctt cgtggcgatt atggcgctgg ttgcagcgtc
    25381 catcatcgaa ccgggtcttt acttcgcgat gaacaccccg cctgctggcc ttggcatcac
    25441 catgcctaac ctgcatgaaa tgggtggcga gaacgcgccg atcatcatgg cgcagctgaa
    25501 agacgttacc gcacacgcgg cagcgaccgt cagctcctgg ggcttcgtga tttcgccaga
    25561 gcagatcctg caaaccgcga aagacattgg tgagccttct gtcctgaacc gtgcaggtgg
    25621 tgcgccgacg ctggcggtag gtatcgctca cgtgttccac aaagtgctgc cgatggctga
    25681 catgggcttc tggtatcact tcggtattct gttcgaagcc ctgttcatcc tgaccgcgct
    25741 ggatgcgggt acccgttctg gccgctttat gctgcaagac ctgctgggta acttcatccc
    25801 gttcctgaaa aaaaccgact ctctggttgc cggtatcatc ggtactgcgg gctgtgtggg
    25861 tctgtggggc tacctgctgt atcagggcgt ggtcgatccg ctgggcggcg ttaagagcct
    25921 gtggccgctg ttcggtatct ccaaccagat gctggcagcc gtagcgctgg tactgggcac
    25981 cgttgtgctg attaagatga agcgcaccca atacatctgg gtaactgttg ttccggctgt
    26041 atggctgctt atctgcacca cctgggcgct gggtctgaaa ctgttcagca ccaacccgca
    26101 gatggaaggc ttcttctaca tggcaagcca gtacaaagag aagattgcta acggtactga
    26161 cctgacggcg cagcagattg ccaacatgaa ccacatcgtt gtgaacaact acaccaacgc
    26221 aggcctgagt attctgttcc tgattgtggt gtacagcatc atcttctacg gtttcaaaac
    26281 ctggcttgcg gtgcgtaaca gcgacaaacg tactgacaaa gaaacaccgt acgttccaat
    26341 cccggaaggc ggcgtgaaga tctcttcgca ccactaaccg tgtttagccc cgcttcggcg
    26401 gggctttgtt cttatcagag tgaactatgt ttggtaactt aggacaggca aaaaaatatc
    26461 tcggccaggc ggcgaagatg ttgattggta ttccagacta cgacaactac gtcgagcata
    26521 tgaagaccaa ccatcccgac aagccgtaca tgagctatga agaattcttc cgcgaacgcc
    26581 agaacgcgcg ctacggcggc gatggtaaag gcggtatgcg ctgttgttaa tggagagagc
    26641 atgaacccga ttgcagttac cctactcacc ggttttttag gcgcgggcaa aactaccctg
    26701 ctgcgccata ttcttaacga acaacacggc tacaagattg ccgtgattga aaacgaattc
    26761 ggcgaagtct ccgttgatga tcaactgatt ggcgaccgcg ccacgcagat caaaacgctg
    26821 accaacggct gcatctgctg ttcgcgctcc aacgagctgg aggacgcact actcgacctg
    26881 ctggataatc tcgacaaggg caatattcag ttcgatcgtc tggtgattga atgcaccggc
    26941 atggccgatc ccggcccgat tattcagacc tttttctccc atgagatttt atgccagctc
    27001 tacctgctgg acggcgtgat tgcgctggtg gatgcggtac atgccgatga gcagatgaac
    27061 cagttcacca tcgcccagtc ccaggtgggc tacgccgacc gtattctgct gactaaaacc
    27121 gacgtcgcag gcgaagcaga gaaactgcgt gaacgcctgg cgcgcatcaa cgcccgcgca
    27181 ccggtctaca ccgtcaccca cggcgacatc gacctgggcc tgctgttcaa caccaacggt
    27241 tttatgctgg aagagaacgt cgtcagcacc aaaccgcgtt tccactttat cgcggataaa
    27301 caaaacgata tttcgtcaat tgtggtggaa ctggattacc cggtagatat cagcgaagtt
    27361 tcccgcgtga tggaaaacct gctgctggaa tcggcggata aactgctgcg ttacaaaggg
    27421 atgctgtgga ttgacggcga acctaaccgc ctgctgttcc agggcgtcca gcgcctctac
    27481 agcgccgact gggacaggcc gtggggcgat gaaaaaccgc atagcacaat ggtgtttatc
    27541 gggattcagt tgccggagga agagattcgg gcggcgtttg cgggattgag gaagtaagtt
    27601 cgggaaagat caactttttg agagttccac cgcaacatta ttacgagtgg aactctttag
    27661 atatgtcaga ccaaccaaag cttcttcaat tgcgttttca ttttttcatg tctcattttc
    27721 agttcagcca atccccattc tgaagaatta caaacatctt ttgttaaata aaaagagctt
    27781 ttactattaa gtttctcata aatagatttc tttttgataa aatcatagtg ttgtgtttca
    27841 gaatttttgg aaccacttat gagtgttaaa ttacctattt tatgcagcca gtagacatgc
    27901 tcttgaggtt ggaatctatt aatccagtat tcattaacta atgcttgagg aagaatatgc
    27961 tcaattgtga ttctccccgt ataggtttta atcacgctct catcttgttg ctccatatct
    28021 aagcgaagca aaattgcttt tattaagttc acttgattcg gacgaggctc atataaatct
    28081 tcatccagag cagctataaa acctgaatta tctgcatgtt ggtttatctg atttatcaca
    28141 gaatcaaaag gcatatcatt atttattgct accagagcag aataacaaac catttctctt
    28201 tggcttttaa tttgtttttt taaccaccca tgcatatata ccttctcaaa ggcggtaata
    28261 aattgagaaa aatcatctaa attaaaatct tctgtacgag ccattcgatt catgaatgcc
    28321 ataaccggag gaatccactc atctacccct aaattactta gcgatgctat ttttcgtcgt
    28381 attgaagggt gttcaaaatc attctctaaa atcttagtat aattt
//
LOCUS       DAEEUE010000047        28406 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.047, whole genome shotgun sequence.
ACCESSION   DAEEUE010000047 DAEEUE010000000
VERSION     DAEEUE010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28406
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.047"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..142)
                     /gene="yjdI"
                     /locus_tag="KLK84_002190"
     CDS             complement(<1..142)
                     /gene="yjdI"
                     /locus_tag="KLK84_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HBE2009502.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            323..1954
                     /gene="dcuS"
                     /locus_tag="KLK84_002191"
     CDS             323..1954
                     /gene="dcuS"
                     /locus_tag="KLK84_002191"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HBE2009503.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1951..2670
                     /gene="dcuR"
                     /locus_tag="KLK84_002192"
     CDS             1951..2670
                     /gene="dcuR"
                     /locus_tag="KLK84_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HBE2009504.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3241..4581
                     /gene="dcuB"
                     /locus_tag="KLK84_002193"
     CDS             3241..4581
                     /gene="dcuB"
                     /locus_tag="KLK84_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HBE2009505.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4659..6305
                     /gene="fumB"
                     /locus_tag="KLK84_002194"
     CDS             4659..6305
                     /gene="fumB"
                     /locus_tag="KLK84_002194"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBE2009506.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQIAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6428..7057
                     /locus_tag="KLK84_002195"
     CDS             6428..7057
                     /locus_tag="KLK84_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HBE2009507.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALFGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7196..8617)
                     /gene="melB"
                     /locus_tag="KLK84_002196"
     CDS             complement(7196..8617)
                     /gene="melB"
                     /locus_tag="KLK84_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HBE2009508.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8732..10075)
                     /gene="melA"
                     /locus_tag="KLK84_002197"
     CDS             complement(8732..10075)
                     /gene="melA"
                     /locus_tag="KLK84_002197"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBE2009509.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPS"
     gene            10358..11266
                     /gene="melR"
                     /locus_tag="KLK84_002198"
     CDS             10358..11266
                     /gene="melR"
                     /locus_tag="KLK84_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HBE2009510.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11465..13732
                     /gene="adiA"
                     /locus_tag="KLK84_002199"
     CDS             11465..13732
                     /gene="adiA"
                     /locus_tag="KLK84_002199"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HBE2009511.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            14057..14818
                     /gene="adiY"
                     /locus_tag="KLK84_002200"
     CDS             14057..14818
                     /gene="adiY"
                     /locus_tag="KLK84_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HBE2009512.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            14955..16292
                     /gene="adiC"
                     /locus_tag="KLK84_002201"
     CDS             14955..16292
                     /gene="adiC"
                     /locus_tag="KLK84_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HBE2009513.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            16396..18039
                     /gene="eptA"
                     /locus_tag="KLK84_002202"
     CDS             16396..18039
                     /gene="eptA"
                     /locus_tag="KLK84_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HBE2009514.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRATNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            18036..18704
                     /gene="basR"
                     /locus_tag="KLK84_002203"
     CDS             18036..18704
                     /gene="basR"
                     /locus_tag="KLK84_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HBE2009515.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            18705..19805
                     /gene="pmrB"
                     /locus_tag="KLK84_002204"
     CDS             18705..19805
                     /gene="pmrB"
                     /locus_tag="KLK84_002204"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HBE2009516.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19781..19870)
                     /gene="pmrR"
                     /locus_tag="KLK84_002205"
     CDS             complement(19781..19870)
                     /gene="pmrR"
                     /locus_tag="KLK84_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HBE2009517.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(19982..21484)
                     /gene="proP"
                     /locus_tag="KLK84_002206"
     CDS             complement(19982..21484)
                     /gene="proP"
                     /locus_tag="KLK84_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HBE2009518.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(21748..22626)
                     /locus_tag="KLK84_002207"
     CDS             complement(21748..22626)
                     /locus_tag="KLK84_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HBE2009519.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(22623..24851)
                     /gene="crfC"
                     /locus_tag="KLK84_002208"
     CDS             complement(22623..24851)
                     /gene="crfC"
                     /locus_tag="KLK84_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HBE2009520.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            25253..25588
                     /locus_tag="KLK84_002209"
     CDS             25253..25588
                     /locus_tag="KLK84_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HBE2009521.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            25746..26189
                     /gene="yjdN"
                     /locus_tag="KLK84_002210"
     CDS             25746..26189
                     /gene="yjdN"
                     /locus_tag="KLK84_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HBE2009522.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            26322..27110
                     /gene="phnC"
                     /locus_tag="KLK84_002211"
     CDS             26322..27110
                     /gene="phnC"
                     /locus_tag="KLK84_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HBE2009523.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            27135..28151
                     /gene="phnD"
                     /locus_tag="KLK84_002212"
     CDS             27135..28151
                     /gene="phnD"
                     /locus_tag="KLK84_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBE2009524.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            28257..>28406
                     /locus_tag="KLK84_002213"
     CDS             28257..>28406
                     /locus_tag="KLK84_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HBE2009525.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMATF"
BASE COUNT         7297 a         7422 c         6934 g         6753 t
ORIGIN      
        1 acgactttcg tttgagatta aataacttgc cgttgccacg tacgcaattg ccagaatgct
       61 gacatatcgc agtgttgaaa tagacatcga tcttttcgcc ggtataacag cgataacccc
      121 cgtccagtag cgcctgatcc atgaccttcc ctctctcatc agtatgatta tcgctataag
      181 catagccccg gaatcttcat acagcactgg cagtttccgt tgccagagtc tcttccccta
      241 tatagccaat catttttact ccctttgaat tacccgcctc atcagagata atgcttaaga
      301 aatttgtcac acaaggaagc tgatgagaca ttcattgccc taccacatat tacgcaaacg
      361 tccgatgaaa ttgagtacca ctgtgatctt aatggtcagc gcggtactgt tctcggtgct
      421 attggtggtg catctgattt acttctcgca aatcagtgat atgacgcgag acgggctggc
      481 caacaaggca ctggcagtgg cgcgtaccct cgccgactcg ccggaaatcc gtcagggctt
      541 gcagaaaaaa ccgcaggaga gtggcatcca ggccatcgcg gaagccgtgc gcaaacgcaa
      601 cgatctgctg tttattgtcg ttaccgatat gcaaagtctt cgctactcac atcctgaagc
      661 ccagcgtatt ggtcagccat ttaaaggtga tgacatcctt aatgcgctga atggcgaaga
      721 aaatgtcgct atcaatcgcg gttttctggc gcaggcttta cgcgtattta cccccatcta
      781 cgatgaaaat cataaacaaa ttggcgtggt ggcgatcggc cttgagttaa gccgcgtgac
      841 ccaacagatc aatgacagtc gctggagcat tatctggtcg gtattatttg gcatgctggt
      901 cggactgatt ggcacctgca ttctggttaa ggtactgaaa aaaatccttt tcggcctgga
      961 accctacgaa atctccacgc tgtttgagca acgccaggcc atgttgcagt ctatcaaaga
     1021 aggcgtcgtt gccgtggacg atcgcggcga ggtcacgctg atcaacgatg ccgcacaaga
     1081 attgctgaat taccgtaagt cgcaggacga tgagaaactg tcgacgctca gccactcatg
     1141 gtcacaggtg gtagatgtct cggaagtgtt acgcgacggt acgccgcgcc gcgacgaaga
     1201 gattacgatt aaagaccggc tattactgat caacaccgtt ccggtgcgca gtaatggcgt
     1261 tatcatcggt gccatttcaa ccttcaggga caaaactgaa gttcgtaaac tgatgcagcg
     1321 actcgacggt ctggtcaact atgctgacgc acttcgtgaa cgatcccacg aatttatgaa
     1381 taaattgcat gtgattctcg gattattgca tctgaagagt tataagcagt tggaagatta
     1441 cattctcaaa acagccaata actatcagga agagattggc tctctgctgg gtaagatcaa
     1501 atctccggtt atcgctggtt ttttaatcag caagattaac cgcgcgaccg atttaggcca
     1561 tacgctgatt ttaaacagtg aaagccagct gccagacagc ggcagtgagg accaggtcgc
     1621 gacgctgatt accacattgg gaaatctgat agaaaacgcg ctggaagcat tagggccgga
     1681 acccggtggc gaaattagcg taacattgca ctaccgtcac ggctggctgc actgcgaagt
     1741 taacgatgat ggaccgggga tcgcacccga caaaatcgat cacatttttg acaaaggtgt
     1801 ctcgacaaaa ggaagcgagc gaggcgtcgg tttagcactt gtcaaacaac aggtagaaaa
     1861 tctcggcggc agcatcgccg tggaatcgga accggggatt ttcacacaat tttttgtcca
     1921 gataccctgg gacggggaga ggtcgaacag atgatcaatg tattaattat cgatgacgac
     1981 gcaatggtcg cggagctgaa tcgccgatac gtagcacaaa tcccaggctt tcaatgctgt
     2041 ggaacagcct cgacgctgga gaaagccaaa gagattatct tcaatagcga tgcgcctatc
     2101 gacctgatat tgctcgatat ctatatgcaa aaagagaacg ggctcgattt actgcctgtc
     2161 ctgcataacg cgcgttgcaa aagtgatgtg attgtcatct cctccgcagc cgatgcggca
     2221 accattaaag attcgctgca ttacggtgtc gtggattacc tgatcaaacc cttccaggct
     2281 tcccgctttg aagaggcgct caccggctgg cggcaaaaga aaatggcgct ggaaaaacat
     2341 cagtattatg accaggccga gcttgatcag ctaatccacg gcagcagctc caacgaacag
     2401 gatcctcgtc gcttgccaaa aggcttaacg ccgcagacgt tacgcacgct gtgccagtgg
     2461 attgacgcgc atcaggacta tgagttttca accgacgagc tggctaacga ggttaacatc
     2521 tcgcgtgttt cctgccgaaa atacctcatc tggctggtca actgccacat cttgttcacc
     2581 agtatccatt atggcgtcac ggggcgtccg gtttaccgtt accgtattca ggcagaacac
     2641 tactcactgc tgaaacaata ttgccaataa cggatcgctg gttatctgta agtaataatt
     2701 attaccgtaa tgcaaaatac ccctggcaca agttacatcg ttaatcagta aaacttgtgc
     2761 cagatcaaat ataattatcc ctccatcatc gttaaaaatt aatatctctt cagatgaacg
     2821 gtgtttttaa tttcaaaacg ataacaaaag ttaattaact attatgtcac ccgcattatg
     2881 tgtattttta cccacaaatg ggtagatcag attaatctat aaacctaatg atatctgccc
     2941 tgagaacaaa aaatagaccg ataaatatca ataagataac agcaaacaaa acattaacat
     3001 ctgcgcagta caaactataa acccatcgcc agagagtttt tctctctgaa aaagccgctt
     3061 atcacagtgc ataaatttgc cgctgcttta atcagccaat attcactgtg aggtatttgc
     3121 taaagccggt aacgaccaaa cggatattta gtcaggctct gaaaacagtt catacaaaac
     3181 agaacgtgac tgtgatctat tcagcaaaaa tttaaatagg attatcgcga gggttcacac
     3241 atgttattta ctatccaact tatcataata ctaatatgtc tgttttatgg tgccagaaag
     3301 ggtggtatcg cgctgggttt attaggcggt atcggtctgg tcattctggt cttcgtcttc
     3361 caccttcagc caggtaaacc accagttgat gtcatgctgg ttatcattgc ggtggtggcg
     3421 gcatcggcga ccttgcaagc ttcgggcggt cttgatgtca tgctgcaaat tgccgagaag
     3481 ctgctgcgcc gcaacccgaa atatgtctca attgtcgcgc cgtttgtgac ctgtacactg
     3541 accattcttt gcggtacggg tcatgtggtt tacaccattc tgccgatcat ctacgacgtc
     3601 gccattaaga acaacatccg tccggaacgt ccgatggcgg caagttctat cggtgcacag
     3661 atggggatta tcgccagtcc ggtgtcggtt gcggtcgtgt ctctggttgc gatgctgggt
     3721 aatgtcacct ttgatggtcg ccatcttgag ttcctcgatc tgctggcaat caccattcca
     3781 tcgacgttaa tcggtatcct ggcgatcggt atcttcagct ggttccgcgg taaagatctg
     3841 gataaagacg aagagttcca gaaattcatc tccgtaccgg aaaaccgtga gtatgtttac
     3901 ggtgataccg cgacgctgct ggataaaaaa ctgccgaaaa gcaactggct ggcaatgtgg
     3961 attttcctcg gggcaatcgc tgtagtcgcc cttcttggtg ctgattcgga cctgcgtcca
     4021 tccttcggcg gcaaaccgct gtcgatggta ctggttattc agatgtttat gctgctgacc
     4081 ggggcgctga ttattatcct gaccaaaacc aatcccgcgt ctatctcaaa aaacgaagtc
     4141 ttccgttccg gtatgatcgc catcgtggcg gtgtacggta tcgcatggat ggcagaaacc
     4201 atgttcggtg cgcatatgtc tgaaattcag ggcgtactgg gtgaaatggt gaaagagtat
     4261 ccgtgggcgt atgccattgt tctgctgctg gtttccaagt ttgtaaactc tcaggctgcg
     4321 gcgctggcgg cgattgttcc ggtcgcgctg gcgatcggcg ttgatccggc atacatcgtg
     4381 gcttcagcac cggcttgcta cggttattac atcctgccga cttatccgag cgatctggca
     4441 gcgattcagt ttgaccgttc cggcaccacc cacatcggtc gcttcgtcat caaccacagt
     4501 tttattctgc cggggttgat tggtgtgagc gtatcgtgcg tcttcggctg gatcttcgcc
     4561 gcgatgtacg ggttcttata aatgcacttt gcgtgccgcc cgtgactacg cggcacgcca
     4621 ttttcgaata acaaatacag agttacaggc tggaagctat gtcaaacaaa ccctttatct
     4681 accaggcacc tttcccgatg gggaaagaca ataccgaata ctatctactc acttccgatt
     4741 acgttagcgt tgccgacttc gacggcgaaa ccatcctgaa agtggaacca gaagcactga
     4801 ccctgctggc gcagcaagcc tttcacgacg cttctttcat gctacgtccg gcacaccaga
     4861 aacagattgc ggctattctt cacgatccag aagccagcga aaacgacaag tacgtggcac
     4921 tgcaattctt aagaaactcc gaaatcgccg ccaaaggcgt gctgccgacc tgccaggata
     4981 ccggcaccgc gatcatcgtc ggtaaaaaag gccagcgcgt gtggaccggt ggcggtgatg
     5041 aagaagcgct gtcgaaaggc gtctataaca cctatatcga agataacctg cgctactcgc
     5101 aaaacgcggc gctggacatg tacaaagagg tcaacaccgg cactaacctg cctgcgcaaa
     5161 tcgacctgta cgcggtagat ggcgatgagt acaaattcct ttgcgttgcg aaaggcggcg
     5221 gctctgccaa caaaacgtat ctctaccagg aaaccaaagc cctgctgact cccggcaaac
     5281 tgaaaaactt cctcgtcgag aaaatgcgta ccctcggtac tgcagcctgc ccgccgtacc
     5341 atatcgcgtt tgtgattggc ggtacgtctg cggaaaccaa cctgaaaacc gtcaaattag
     5401 caagcgctca ctattacgat gaactgccga cggaagggaa cgaacatggt caggcgttcc
     5461 gcgatgtcca gctggaacag gaactgctgg aagaggccca gaaactcggt cttggcgcgc
     5521 agtttggcgg taaatacttc gcgcacgaca ttcgcgttat ccgtctgcca cgtcacggcg
     5581 catcctgccc ggtaggcatg ggcgtctcct gctccgctga ccgtaacatt aaagcgaaaa
     5641 tcaaccgcga aggtatctgg atcgaaaaac tggaacacaa tctaggtcag tacattccac
     5701 aagaactgcg tcaggccggt gaaggcgaag cggtgaaggt cgaccttaac cgcccgatga
     5761 aagagatcct cgcccagctt tcgcaatatc cggtttccac tcgtttgtcg ctcactggca
     5821 ccattattgt gggtcgagat attgcacacg ccaagctgaa agagctgatt gacgccggta
     5881 aagaacttcc gcagtacatc aaagatcacc cgatctacta cgcgggtccg gcgaaaaccc
     5941 ctgccggtta tccatcaggt tcacttggcc caaccaccgc aggtcgtatg gactcctatg
     6001 tggatctgct gcaatcccac ggcggcagca tgatcatgct ggcgaaaggc aaccgcagcc
     6061 agcaggttac cgacgcgtgt cataaacacg gcggcttcta cctcggtagc atcggcggtc
     6121 cggcggcggt actggcgcag cagagcatca agcatctgga atgcgtcgct tatccggagc
     6181 tgggtatgga agctatctgg aaaatcgaag tagaagattt cccggcgttt atcctggtcg
     6241 atgacaaagg taacgacttc ttccagcaaa tcgtcaacaa acagtgcgcg aactgcacta
     6301 agtaacctct tcggcccagc gcctgacagc atgctgccag gtgatccccc tgggccacct
     6361 cttttgcgat tgtaatttca tgcttgctgg tgaatagtca gtattttccc tgacttgcga
     6421 actcaccatg acccgcacac tcaagccgtt aattcttaac accggcgcac tggcgctaac
     6481 gttaatcctg atttataccg gcatttcggc ccatgacaaa ctcacctggc tgctggaagt
     6541 gacaccggtg attatcgtcg tgccgctact acttgccacc gccagacgtt acccgttaac
     6601 gctgctgctc tatacgctca tcttctttca cgccatcatc ctgatggtag gcgggcagta
     6661 cacctacgcg aaagtccccg ttggttttga ggtgcaggaa tggctggggt tgagtcgtaa
     6721 tccgtatgac aagctggggc actttttcca ggggctggtg cctgcactgg tggcacgaga
     6781 aattctcgtt cgcgggatgt acgtgcgcgg acgtaaaatg gtggcgtttc tggtctgttg
     6841 cgtggcgctg gcgataagcg cgatgtatga attaatcgag tggtgggcgg cactggcgat
     6901 gggccaggga gcggatgatt ttctcggtac ccagggcgac cagtgggaca cgcaatccga
     6961 tatgttctgc gcgctgtttg gcgcattaac gacagtgata ttgctcgctc gctttcattg
     7021 ccgccagcta cggcgctatg gcttgataac tggataacaa cgtgcacttt gttgatgccg
     7081 gatgcggcgt gaattcctta tccggtctac aaaatttgca aatccaaagc attacattaa
     7141 gacgtggcct gataaacata tcgcatcagg ccattttcgt tagctattgt ccacgtcagg
     7201 ctttcacatc actcactgca ccgaccggaa tatcagcatg aacaggctcg ggcggtactt
     7261 tgcgatattt atccagcaga tgaatctgga tcctgcgcag cgtgtcgcca ttgaggcgat
     7321 agaaacggaa gtagagaatc agcgttacca tgaaaaacag agtcggtaga gcaatcataa
     7381 taaactgcat acctaatagg gcttgcgtag actgttcaac attcggtaca tagccaatca
     7441 tccctaacac aaccgcaatg aaaaaagccg caaaggctga accgcccttc accaccatag
     7501 tctgcacgga gtaagcgatg ctttcacagc gtacgtgcag tttatattca ccgtaatcaa
     7561 cggtatctgc caccatgatg acctgtaata cccagaaaag cgccgttccc acattcagca
     7621 aaatacccgc aatcacaatg aggacgacgt tgtgatagct cattaatgcc attaacagga
     7681 gaacaccaca gcttaacacc ggaagaatag atgctccggc ccataaaatg cgtcgggata
     7741 atgatttaac taagcgtggg aagaatacta acgtcaccag gttagcagct cccgcatacg
     7801 acagataata ggggaacaaa tccgcatcac cgataacata tgagaaataa tagatagcaa
     7861 atccggtaat aatgttgctg gctacattat aagcaagagc catacccaag aggcatgaaa
     7921 gctgatcgtt tttataaata agcgcaacga tggctttaag tgtcagatgg cttccttcag
     7981 cagacgattg attgtctgac gaaaagactt catgcacatt gcgcagagtg atgatggttg
     8041 aaacaataaa aaaggcgatc aataccagag tgaacatctg aaagccaaat ccccgatcgc
     8101 caccgccgac ataattaaca aatggtagcg tcacacctgc cgtaacaaag cctgccagac
     8161 tggcaaaaaa acgcggataa ggaaccagtt gttcgcgttc acgtttatcg agcgtgatgg
     8221 ttggaaccag cgaccagaag ggaatatcca taatggtgta agtcatgccc cagaggatgt
     8281 aggtcacgca aacaaagaca atctgagtag taccttcaaa cagatgcgca ctaaagagga
     8341 gaaataagat tacagagttt gccaacgtac cgatcaggat ccagggtttg aacttacccc
     8401 atcgcgatcg cgtagcattt acaatccatc ccataatcgg atcgttaata gcatcccaga
     8461 tcctcgccac cagaaacaaa gtaccgacca aacccacaga cagcccgacg acatcggtgt
     8521 agtaatacat gaggtacata tacacaatgc cgatcgcaaa atccttcccg aacgctccaa
     8581 atccataact gagttttgta gtcattgaaa tgctcatagg gtatcgggtc gctgttacca
     8641 acgcccgcct tctgttattg gtatcgtcag aggtgtgcgc cggaataaat cccccggcgc
     8701 agtagcgttt agtcgcgttt taacggtgca actagcttgg cagccagtcg ccgtgggcgg
     8761 caatcaggtc gtcaacaaga gcatatattt cgtcaatgcc cagcacggcg gcagtatgcg
     8821 ggtccatcat cgcggcgtgg taaacacggt cgcgattttc cgtaagaata gcttcggtca
     8881 gcagcgtctg tacgttgatg ttggtttgca tcagggcggc cagatgcgaa ggtagcgtac
     8941 cgactttggt cggctgaatg ccattagcat caaccagaca ggctacttcc acgcaacatc
     9001 cttgtggcag gttatcaatc aaaccatcgt tacggacgtt gccgtaaatc acactcggct
     9061 cgccagtcca gatagcgttc atgattgtgc tggcatattc ccgtgacggt ttaatatcaa
     9121 tccgggaggc gtttttatac tcctccagct ctttatgcca gttcgccagc tgctcgacgc
     9181 agcgtttcgg gtactcatcc agcggtactt tataacgctc aatcaaatcc tcacgaccta
     9241 gcttaataaa ccacggcgta tattcggcaa aatgttctga cgattccgtg acgaagtagc
     9301 ccagcttttt gaacatttcg tagcgcacaa tattctggca gcgagtatta ccatgaatat
     9361 ttggcttcgg tgcctgccct gcttcataag ccgccagcag ttccgggtag agattcacgt
     9421 aactgccgtc ggcggttttg cgctccagct ccaggtaaaa cgccatatgg ttgatacctg
     9481 cgcaacggta acgcagcgta gccgggtcga tattgaggtc acgcgccagc tcttccgccg
     9541 ttccctgcac cgaatggcac agcccgacct gtttgatata cggatagcgg gcatacatcg
     9601 cccaggtatt catcgccatt gggttaacat agttgagcat ggtggcatcg gggcagactt
     9661 ccgtcatgtt ctcgcaaatt tgccacagat gcggaatggt acgtagcgcg cgcataatac
     9721 cgcccggccc caacgtatcg gcaatggttt gttccagacc atgccgctta cagacctcga
     9781 aatcagtcac cgtgcaaggt tcataaccgc caatctgaaa tgccaccacg acaaaatcgg
     9841 catcctctaa ggcttctttc tgttgggtgt ggcaggtgat tttgccgctg gcccctgctg
     9901 aatccatcag cttacgcacc acaatatgcg actcttccag gcgggtgggg tcaatgtcca
     9961 tcagggcaat atgcgccgtt ttcagcgcct cgcgatggaa cacatcacca agaatatttt
    10021 taacgaaaat cgtcgaacca gcgccgataa atgtaatttt gggtgcagac atcatgcaga
    10081 tctcctggct tgcttgaata acttcatcat ggcaggcgaa tctgcagaaa acctccggct
    10141 tccaggttga gcattcccat aaactcagat cctgcgtgaa gcagcagtaa atctgagttt
    10201 atgggaataa attacctgaa aagtagagtt ttcggcctca tgatgctcgg acagtcccct
    10261 cgcccctctg ggaagagggt gaaaaccgtg ctcccactcg cagtcatcct ccctcactcc
    10321 tgccataatt ctgatattct aggaaagaga gccatccatg aatacagata cgtttatgtg
    10381 cagcagcgac gaaaaacaga cccgcagccc gctgtcgctg tattcggaat accagcggat
    10441 ggagattgag tttcgcgcac cgcatatcat gcccaccagc cactggcatg gtcaggtcga
    10501 agtgaatgtg cctttcgatg gcgatgtgga atacctgatc aacaatgaaa aagtgaatat
    10561 caatcagggt catatcacgc tgttctgggc ctgtacaccg caccaactaa cagataccgg
    10621 aacctgtcag agcatggcga tttttaatct gccgatgcat ctgtttctct cctggccgct
    10681 ggataaagac ctgattaacc acgtcactca cggcatggtg atcaaatcac ttgccacaca
    10741 gcaacttagc ccgtttgaag tacgccgctg gcagcaggaa ttaaacagtc cgaatgagca
    10801 aattcgccag ctcgccattg atgaaattgg cctgatgctc aagcgattta gcctctctag
    10861 ctgggaaccg attctggtca ataaaacctc gcgcacacac aaaaacagcg tctcgcgcca
    10921 tgcgcaattt tatgttagcc agatgctggg ctttattgcc gaaaactatg atcaggcgct
    10981 gaccatcaac gatgtggctg agcacgtcaa acttaacgcc aactatgcaa tggggatatt
    11041 ccagcgggtc atgcaattga cgatgaaaca gtacattacc gcgatgcgca tcaaccacgt
    11101 tcgcgcgtta ctgagcgata ccgataaaag tattctcgat attgccctga cggcaggctt
    11161 tcgttcaagt agtcgttttt acagcacgtt cggcaaatat gtcggcatgt cgccgcaaca
    11221 ataccgcaaa cttagccaac agcgccgcca gacgtttccc ggctaaagac gatatcagta
    11281 tcagccaaaa aaatagttta gccgtcgata ataagcagtg aaaataattt aaaataatca
    11341 cataacgtat tgtttataaa actaaatatt tcaaaaatgt ttgtttttca cgcgctttac
    11401 agcccgaaaa ggccggaaga tacttgcccg caacgaagat tccttcataa ccgggtaagc
    11461 aatgatgaaa gtattaattg ttgaaagcga gtttctccat caagacacct gggtcggtaa
    11521 cgccgttgag cgtctggcag atgctttaag ccagcaaaat gttaccgtga ttaaatccac
    11581 ctcctttgat gatggttttg ccattctctc ttcaaacgaa gccattgact gcctgatgtt
    11641 cagctatcaa atggagcatc cggacgaaca tcaaaacgtc agacaattga tcggtaagct
    11701 tcatgagcgc caacaaaacg tgccggtctt cctgttgggc gatcgggaaa aagccctcgc
    11761 cgcaatggac cgggatctgc tggagcttgt cgatgaattc gcctggattc tggaagatac
    11821 cgccgacttt atcgccggac gcgccgttgc cgcgatgacc cgctaccgcc agcagctgtt
    11881 gccgccactg ttcagcgcgc tgatgaaata tagtgacatc catgaatatt cctgggcagc
    11941 gccaggccac cagggcggcg ttggttttac caaaacaccc gccggacgtt tctaccatga
    12001 ctactatggt gaaaatctgt tccgcaccga catgggcatc gaacgaactt ccctcggttc
    12061 tttgcttgac catactggcg catttggcga aagcgaaaaa tatgccgcac gcgtatttgg
    12121 tgccgatcgc tcctggtcgg tagtcgtcgg tacttccggc tctaaccgca ccatcatgca
    12181 ggcttgcatg accgataacg atgtcgtggt cgttgaccgt aactgccata aatccatcga
    12241 acaaggtttg atgctgacag gcgcgaaacc ggtctatatg gtgccaagcc gcaaccgcta
    12301 cggcattatc gggccaatct atccgcagga aatgcaacct gaaaccttgc aaaagaaaat
    12361 cagtgaaagc ccgctgacca aagacaaagc cgggcaaaaa ccgtcttact gcgtggtaac
    12421 caactgcacc tatgacggcg tgtgttataa cgctaaagaa gcgcaggatc tgctggaaaa
    12481 aacctccgat cgtctgcact ttgacgaagc ctggtacggc tatgcacgtt tcaacccgat
    12541 ctatgccgat cactatgcca tgcgcggcga acctggcgat cacaacggtc ctaccgtttt
    12601 cgccacccac tccatccaca aactgctgaa tgcgctgtca caggcttctt atattcatgt
    12661 acgtgaaggt cgtggggcga ttaacttctc ccgcttcaac caggcctaca tgatgcatgc
    12721 caccacctcc ccgctgtatg ccatctgcgc atccaacgac gtggcggtgt cgatgatgga
    12781 cggcaacagc ggcctgtcac tgacacagga agtgattgac gaagcggttg atttccgtca
    12841 ggcgatggcg cggctatata aagagttcac cgctgacggt agctggttct tcaaaccgtg
    12901 gaacaaagaa gtcgtcaccg acccacaaac cggcaaaacc tatgactttg ctgacgcacc
    12961 aaccaaactg ctgaccaccg ttcaggactg ctgggtaatg catccgggcg aaagctggca
    13021 cggcttcaaa gatattccgg ataactggag tatgctcgac ccaattaaag tcagcatcct
    13081 tgctccggga atgggtgaag atggtgaact ggaagaaacc ggtgttccgg cggcgctggt
    13141 cactgcctgg cttggtcgcc acggcattgt gcctacccgc accactgact tccaaattat
    13201 gttcctgttc tctatgggcg taacccgtgg gaaatggggg actctggtta acaccctttg
    13261 ctccttcaaa caccactatg acgccaacac accgctggcg caggtgatgc cggaacttgt
    13321 tgaacaatat cctgacactt acgcgaacat ggggattcac gatctgggtg acaccatgtt
    13381 tgcctggctg aaagaaaaca accctggcgc acggttgaac gaagcctatt ccggcctgcc
    13441 ggtggcggaa atcaccccgc gtgaagcgta caacgcgatt gtcgacaaca atgtcgaact
    13501 ggtatccatt gaaaatctgc caggacgcat cgcggcaaac tcagttatcc cgtatccgcc
    13561 aggaatcccg atgctgctgt ctggtgaaaa cttcggcgat aaaaacagtc cgcaagtaag
    13621 ttatttacgc tcgctgcaat cctgggacca ccatttccct ggatttgaac acgaaactga
    13681 agggactgaa attattgacg gtatttacca cgttatgtgc gtgaaagcgt aaccactatt
    13741 ccgctgaagg cgtaattgtt taaataacat tacgccgcct ggccttaggc cttttgagta
    13801 tggcaacgtt ttcataaaaa ttgctgcaaa caaaaatgtc atactttttg cgcggcccca
    13861 ccccgcgctt ttttttgcct gttatttatc ctgtaaaaaa atatgtacat gagaaattac
    13921 tataaaaatt tgtaatatta gtaaaactcg ttatttttat gtatgtttat attcatcatg
    13981 caattatata accatttccc ggtatcgctt tgctttagcg agaactggtc ttttattcgc
    14041 actcaggagt acatgtatga ggatttgcag cgaccaacct tgtattgttt tattgactga
    14101 aaaagatgtc tggataaggg tgaatgggaa agaacctatt agccttaaag ctaaccatat
    14161 ggcgttatta aattgtgaaa ataatattat cgacgtctcc tctcttaaca acactttggt
    14221 tgctcatatt agtcacgaca tcatcaaaga ttacctccgg tttctgaata aagatctctc
    14281 gcaaatacca gtatggcaac gtagcgctac gcccatactc accctgccat gcctgacgcc
    14341 agacgtcttt cgcgttgccg cgcaacacag catgatgccc gcagaaactg agtcagaaaa
    14401 ggaacgaaca cgcgcattat tattcacggt gctatcccgt tttctcgaca gtaaaaaatt
    14461 cctttcatta atgatgtata tgttacgtaa ttgtgtaagt gacagcgttt atcaaattat
    14521 tgaaagcgat attcacaaag actggaatct tagtatggta gccagttgtt tatgtcttag
    14581 cccaagtctg ttaaagaaaa agttgaaaag cgaaaacacc agttatagcc aaataatcac
    14641 cacctgccgc atgcgttatg ccgtaaatga attaatgatg gacggtaaaa atatctctca
    14701 ggtatcacag tcctgcggct acaacagtac gtcgtacttt atttctgtct ttaaagactt
    14761 ctacggtatg acgccgctgc attatgttag tcagcacaga gaacgcactg tcgcctgatt
    14821 tttaacctta acgaagagct atattaataa cggcatcagc gataacccgg tcgataataa
    14881 ttcaactatc gaatgcaggc gtatgatatg acgtaattta ttgtcacgaa gctcgccttc
    14941 gcaggagttt aattatgtct tcggatgctg atgctcacaa agtgggctta atccccgtca
    15001 ccctgatggt gtcggggaat attatggggt caggtgtttt tctgttacct gcaaacctgg
    15061 cctccactgg cgggattgcc atttatggat ggttggtgac gattatcggt gcgctggggc
    15121 tctcgatggt atacgccaaa atgtcgttcc tcgacccaag tcctggtggt tcttacgctt
    15181 acgcccgccg ctgctttggc ccgtttctcg gttatcaaac caacgtcctc tactggctgg
    15241 cctgctggat cggcaatatc gccatggtgg tcattggcgt aggatattta agttacttct
    15301 tcccgattct gaaagatcca ttggtattaa ccatcacctg cgtcgtggtg ctgtggatct
    15361 tcgtcctgct gaacattgtc ggtccgaaaa tgatcacccg cgtgcaggcc gttgccactg
    15421 tgctggcgct gatccccatc gtcgggattg ccgtatttgg ctggttctgg ttccgtggtg
    15481 aaacctatat ggcggcatgg aacgtcagcg gcctgggcac cttcggtgcg attcaaagta
    15541 cccttaacgt tacgctgtgg tcgttcatcg gtgtggaaag tgcctccgtt gccgcaggtg
    15601 tggtgaaaaa cccgaaacgc aatgtcccta tcgccaccat tggtggggta ttgattgccg
    15661 ccgtttgcta tgtgctctcc accaccgcga ttatggggat gatccctaat gccgcactgc
    15721 gcgtttctgc ctcgccattc ggtgatgccg cacggatggc gttgggtgac accgctgggg
    15781 ccattgtttc cttctgcgca gctgcgggtt gcttaggttc actgggcggc tggacgttgc
    15841 tggcgggtca aacggcgaaa gccgctgccg atgacggact gttcccaccg atttttgccc
    15901 gtgtaaataa agcgggtacg ccagtggcgg ggctgattat cgtcggtatt ttgatgacca
    15961 tcttccagct cagcagcatt tcaccaaacg cgaccaaaga gttcggtctg gtttcttccg
    16021 tctcggtcat ctttacactg gtgccatatc tttacacctg tgcggcgtta ctgctgctcg
    16081 gccacggtca ctttggtaaa gcacgcccgg catatctggc tgttactacc attgccttcc
    16141 tctactgcat ttgggccgtg gtggggtccg gggcgaaaga ggttatgtgg tcatttgtca
    16201 ccctgatggt catcaccgcc atgtatgcgc tgaattacaa ccggctacat aaaaacccgt
    16261 atcccttaga tgcaccaata agcaaagatt aattcccctt aatccagcaa acataaaagc
    16321 caaccttaag aacttaaggt tggcttaatt ttgctttgcg agcatatgcg cactttgttc
    16381 gatggaaaca ccgtgatgtt gaagcgccta ctaaaaagac cctctttgaa tttactcgcc
    16441 tggctattgt tggccgcttt ttatatctct atctgcctga atattgcctt ttttaaacag
    16501 gtgttgcagg cgctgccgct ggattcgctg cataacgtac tggttttctt gtcgatgccg
    16561 gtcgtcgctt tcagcgtgat taatattgtc ctgacactaa gctctttctt atggcttaat
    16621 cgaccactgg cctgcctgtt tattctgatt ggcgcggctg cacaatattt cataatgact
    16681 tacggcatcg tcatcgaccg ctcgatgatt gccaatatta ttgataccac tccagcagaa
    16741 agttatgcgc tgatgacacc gcaaatgtta ttaacgctgg gattcagcgg cgtgcttgct
    16801 gcgctgattg cctgctggct aaaaatcaaa cctgccacct cgcgtctgcg cagtgttctt
    16861 ttccgtggag ccaatattct ggtttctgta ctgctgattt tgctggtcgc cgcactgttt
    16921 tataaagact acgcctcgtt gttccgcaat aacaaagagc tggtgaaatc cttaagcccc
    16981 tctaacagca ttgttgccag ctggtcatgg tactcccatc agcgactggc aaatctgccg
    17041 ctggtgcgaa ttggtgaaga cgcgcaccgc aacccgttaa tgcagaacga aaaacgtaaa
    17101 aatttgacca tcctgattgt cggcgaaacc tcgcgggcgg agaacttctc cctcaacggc
    17161 tacccgcgtg caactaaccc gcggctggcg aaagataacg tggtctattt ccctaatacc
    17221 gcatcttgcg gcacggcaac ggcagtttca gtaccgtgca tgttctcgga tatgccgcgt
    17281 gagcactaca aagaagagct ggcacagcac caggaaggcg tgctggatat cattcagcga
    17341 gcgggcatca acgtgctgtg gaatgacaac gatggcggct gtaaaggtgc ctgcgaccgc
    17401 gtgcctcacc agaacgtcac cgcgctgaat ctgcctgatc agtgcatcaa cggcgaatgc
    17461 tatgacgaag tgctgttcca cgggctggaa gagtacatca ataacctgca gggtgatggc
    17521 gtgattgtct tacacaccat cggcagccac ggtccgacct attacaaccg ctatccgccg
    17581 cagttcagga aatttacccc aacctgcgac accaatgaga tccagacctg taccaaagag
    17641 caactggtga acacttacga caacacgctg gtttacgtcg actatattgt tgataaagcg
    17701 attaatctgc ttaaagaaca tcaggataaa tttaccacca gcctggttta tctttctgac
    17761 cacggtgaat cgttaggtga aaatggcatc tatctgcacg gtctgcctta tgccatcgcc
    17821 ccggatagcc aaaaacaggt gccaatgctg ctgtggctgt cggaggatta tcaaaagcgg
    17881 tatcaggttg accagaactg cctgcaaaaa caggcgcaaa cgcaacacta ttcacaagac
    17941 aatttattct ccacgctatt gggattaact ggcgttgaga cgaagtatta ccaggccgcg
    18001 gatgatattc tgcaaacttg caggagagtg agtaaatgaa aattctgatt gttgaagacg
    18061 atacgctgtt attgcaggga ctgattctgg cggcgcaaac cgaaggctac gcgtgcgatg
    18121 gcgtgacaac cgcgcggatg gcggaacaaa gccttgagga cggtcattac agcctggtgg
    18181 tactggattt agggttaccc gacgaagatg gactgcattt tctcgcccgt atccggcaga
    18241 aaaaatacac cctgccggta ctgatcctca ccgctcgcga tacgctgacc gacaaaatcg
    18301 ccgggctgga tgtcggtgcc gatgactatc tggtgaagcc ttttgcgctg gaagagttac
    18361 acgcccgtat ccgcgccctg ctacgacgcc ataataatca gggcgaaagt gagctgattg
    18421 ttggcaatct gacgctgaat atgggtcgcc gtcaggtatg gatgggcggt gaagagttga
    18481 ttctgacgcc caaagaatat gctctgctgt cacggttaat gctcaaagca ggcagtccgg
    18541 tacatcggga aattctctac aacgacatct ataactggga caatgaaccc tcgaccaaca
    18601 ccctggaagt gcatatccac aatctgcgcg acaaagtggg caaagcccgt atccgcaccg
    18661 tgcgcggctt tggctatatg ctggtcgcga atgaggaaaa ctaattgaat ctgatgcgtt
    18721 ttctgcgccg accaatatcg ctgcgccaac ggctgatatt gaccatcggg gccattttgt
    18781 tggtgtttga gctgatcagc gtcttctggc tatggcatga aagtaccgag cagattcagc
    18841 tgtttgagca ggcgctacgc gacaatcgca acaacgatcg ccacatcatg cgtgagatcc
    18901 gcgaggcggt cgccagcctg attgtccccg gcgtctttat ggtcagcctg acgctattta
    18961 tctgctatca ggcggtacgc cgcatcaccc gcccgctggc ggagctgcaa aaagagctgg
    19021 aagcgcgcac cgccgacaac ttaacgccca ttgccattca cagcgccacc ctcgaaatcg
    19081 aagcggtggt ttcggcgttg aacgatctgg tcagtcgcct gaccagcacg ctggataacg
    19141 aaaggttgtt taccgctgac gtcgcgcacg aactgcgaac gccactggcg ggggtgcgtt
    19201 tgcatctgga actgctggcg aaaacgcatc acattgatgt agcaccgtta gtggcacggc
    19261 ttgatcagat gatggagagc gtctcccagc tgctgcaact ggcgcgtgcc ggacagtcat
    19321 tttcttccgg taattatcaa catgtaaaac tgctggaaga tgtcattctc ccctcgtatg
    19381 acgaactcag taccatgctc gaccagcgac agcaaaccct gctattgcca gagagcgccg
    19441 ccgacatcac cgttcagggt gatgcaaccc tgctgcggat gttattgcga aacctggtag
    19501 aaaacgccca tcgttacagc ccgcaaggca gcaacattat gattaagctg caagaagatg
    19561 gcggagcggt catggcagtt gaagatgaag gaccaggtat tgatgagagt aaatgcgggg
    19621 agttgagtaa agcgtttgta cgtatggaca gccgttatgg cgggattggt ctggggttaa
    19681 gtattgtcag ccgcattaca cagttgcatc acgggcagtt tttcctgcaa aaccggcaag
    19741 agacttccgg cacgcgggcc tgggtacggc tgaagaaaga tcagaacgtg gcaaaccaga
    19801 tataaagaaa gctgctgacc accagcacgc tgaacacggt agttaaactt tcataaacac
    19861 ggtttttcat tgcactctcc tccgttaacc tggaggagag tatgcgcgtc agagattaag
    19921 tgaaccttaa gagttcaatt ggcctgataa gacagcgtca catcaggcca tccgtttcag
    19981 cttattcatc aattcgcgga tgttgctgca ccaggcgggt acgtttcgcc tgcaaatcgg
    20041 caatctcgtg gtcaatatta tcgattttct gctcgatatt atcgtaatgc tcgacgagaa
    20101 tttccttcgc ttcctgtata tctgacgccg ccggtgtcgc acctttcaac ggacgatttg
    20161 ccgtctcttt catggttacg ccggtgatta aaccaatcac cgccactacc atcaggtaat
    20221 aggcaggcat catcagattc tgcgagcttt cgaccagcca ggccgccagt gttggcgtca
    20281 gaccggcaac cagcaccgaa atattaaatg ccgccgccag cgcgctgtaa cggatatgcg
    20341 tcgggaacat cgctggcaag gtagaagcca taacgcccat aaagcagtta aggatcaccg
    20401 ccagcatcag taacccggca aaaatcaggc cgatgacgtt actgttaatc agaataaacg
    20461 ccgggatcgc caacacaaac agtgcaacac taccaagtag cacaaacgga cgacggccaa
    20521 aacggtcact cagcaagccc atcaccggct ggacaaacag cataccgatc ataatggcga
    20581 taataatcag caccccgtgg tcttcggagt aatgcaggtt atgcgacaaa taactcggca
    20641 tataggtcag caacatgtag taagtcacgt tggtggcaat taccagacca atacatgtca
    20701 acaggctgcg ccagtattta gtggcaatct ctttaaacga gactttcggg ccatcctgca
    20761 aaccttcgcg gtcgccctgt tccagtttat caacatgctg ctggaacgcc ggagtttctt
    20821 ccagcgcatg gcgcaggtaa agcccgataa tccctaacgg cagagcaata aagaacggaa
    20881 tacgccagcc ccagtcgagg aagttcgctt cgccgacaat ggtcgaaatt aacaccacca
    20941 cgcccgcacc cagcacaaac ccggcaatag aaccgaagtc cagccagctg cccataaagc
    21001 cacgtttacg gtcaggggag tattccgcaa caaatatcga cgccccggta tattcaccac
    21061 cgaccgagaa accttgtgcc atcttacaga tcaacagcag aatcggtgcc caaataccaa
    21121 tcgtgtcgta ggacggtatt aagccaatac agaacgtact gatcgacata atcacaatag
    21181 tgatagcgag gatcttctgg cgaccatatt tatcgcccaa cataccaaag aagagtccgc
    21241 caagcggtcg aatcagaaag ggaacggaga aagtggcaag tgcagcaacc atctgcacgc
    21301 tggggtcagc ccccgggaaa aaaactttac ctaatgcgta agcaacaaaa ccataaacac
    21361 caaaatcaaa ccattccatt gcattaccca gtgatgctgc ggtaatggct ttacgcagtt
    21421 taccgtcatc aataatggtg acatcacgaa gggtaatcgg ttttactttt ttccttttca
    21481 gcatagcttt cctcgcagag ttggcccatt tccgcaaacg ccagaaaaat cagcgcgacg
    21541 gccgcaaaag gggagcggga attgcaggat gcaatctctg taacaagcgt agcgggtttg
    21601 cttacaccct ccggttaagg aatgtacaat caaaccagtt gacgcctgaa ttgggcagtt
    21661 aatgaccttt ttaccctacc agtgtttaaa tttgtgatca acttcacaca tggatttcgt
    21721 ttaggactca ttgatgtaac tgattattta cgccaaattt acagagaaga tttcctcgac
    21781 cattgccccc accaaacgct gcgctgaaca gagtcgtggc atccccagcg cgacagtttg
    21841 ccagcgttgg gtcactgacc agcaaacggg ttgtttatct gccgtggtcc actttcccag
    21901 ccaacttaaa aaatcagcat gatccccgcc cgtcggaacg gtcgaagagg caaaccactg
    21961 atggtaatag tgacgcgtag tcggtgccgc attgatactc tctgccagat gttgtgtcgc
    22021 tttctcgcgc aaatcaccca gcaacaacac caccgcttgc gggcttccca gacggcaggc
    22081 atcgccaaat gcaccccagc gcaactcctc cagaacgata aaacagcgcc caggcaacga
    22141 ccaggcgtta tacgccccag cacgccagcg ggtaaaaatt tgctcgctct gatcacctgc
    22201 ctgcaccagt aggcctcgtt gcgttaacgc cttttccttc cagcgacaac gggctgaaaa
    22261 ccactcatac aaggcggcaa tttgctgggg gagctccggg aaagcagagt gctggcggtg
    22321 atccattttt tgcagcagcg ccagatgctg cgcgatctgc gtcagcgcca gatgaccggg
    22381 atccagcatc gacgccagtt tttccaccat tgtcagtcgc aggctaagcg gcgcatttaa
    22441 catgccgccg ttcgtccatg tctgaagctg cacacgcgaa agaagttcct gcattagtcg
    22501 ttcacgaaac tgctgctgat gggcttgagg atggcgggca tcatcgccct gcgccagatc
    22561 cactaaaaag cgcggatgaa ccgactccag cacgcgaccg gggccgtcca gtaacgtctt
    22621 ggtcaatatc gttctgccgt gaaaagtgtt tgaatatcat cgcgtaacag ctgagcgtcg
    22681 gtgtaaatcc agctgtgagt catcacagtc tgctgcaatt gctgctgcat cagcctgacc
    22741 accgattcat tttgttgacg cagagccaga ctttcgcgta aacgcgtctg taattctgtc
    22801 aaacatgaag cgaactcagc gaaaaaagta ttcatgcctg ccgtaacaga ttcatcgacc
    22861 tgctctgcca gaactttagc catttgttgg caatagagat cgacttctgc gcttaatgct
    22921 cgttgcaaca cactgtaatc aaccgtttct gtcggggatt tctcatttcc ccgtccccag
    22981 tcgggctgat tcaaccagcg cgaaaaagtc tcacgcacaa cgcctaaacg cgtgctctgc
    23041 tcgtccgttg cttcctggcg cgaaatgact tcactgaaca gctggcgagt gttgaagtgg
    23101 ggaactacgc cgtgaaaaac aggaaaatga aacccaggac gaaaccctga ctcgctcaat
    23161 tccattttga cttgttgctc aatggggcga ataacatcgg ttaacacccg gcaaagggtg
    23221 gattccatct cggcaaaacg cagcgtaaag tcgcgactga tggtgttctg cgccgtctgt
    23281 aacagtgtct cacagcgggt acgaatctcg cttaacggct ccgaatcatc ctgaaacaag
    23341 gcggctaact gcgcattcac cgcatcttgc tgttgacgca gaaagtggtt ggcggaggtc
    23401 agggccagct cgatttcatg tttaatctcg ccgctcacct gcgcctgatt gagttgcaat
    23461 agctgcaaac tttcttcgac ctgatggata ttttgccgca attgttcaca agcgacgttt
    23521 aacccgtgcg cacgaaaatc caggtattcc cgcgcctgct gcgcgtaatt caacagttta
    23581 tgcgcagcag atcgcagagc atacaatgag gcgttagcgt aagcggcatg aagcaacgcc
    23641 tgaattggct gggcgaacag cgaatcttcc cacaactgat cggcagcatg acgaatatgt
    23701 tcgaggtccg ccagatcggc atgacgccag cgcctgccga gcgcggcatg ggcaaaatct
    23761 tccacccagc gttgttgctc tggcgctggt aacttaccgt tgttggctaa ctcatagcgc
    23821 gcccgattcg ccaggtagcc ccacatcgac gacaccggga atatctgctg tggcgtaata
    23881 cagcctttca tcagcgtccc ggaaatcagt gcccgcacct ggtcggcgtc gtcactgtta
    23941 cgatcctgtt gatcgaactt attgaccagc acatacagcg gcaccgattg ccccaccgcc
    24001 aaaatcgcct cacggacctc ttcatcggag atcgatttca gttgcgtata atccagcacc
    24061 gccagtaccg ccgaggcgcg tgccagctgc tggttaagca ttttttgcag atgcggttgc
    24121 ccggcttcat ttggcccggg ggtatccagt aacgtcaact gaccgggata actttccagc
    24181 cccgccagat ggacaaactc cacttcaatc acgggaatat gctcaatggc ggcgtaagca
    24241 gaaaaaggaa aatcgacgtc cagcgccttc gccagtcgca ctaaatcatt caaacttttc
    24301 agacaatgaa aaataggctg ggcacccaga taatattttt cgaaagcgac gccattttcg
    24361 atccgctgca taagcgcacg catatcttta tctatttcca gcacatcggt cagatgctta
    24421 atatcgcaat cacgcaggcg ctgttgtaat tgttgaatta aacaatcgat tggcgcgaca
    24481 tgtgaaaaat gcagtaccgg ttccttttgc ccgggcgtat ggcgaataag cgtcggcagc
    24541 gcagtcattg ggcgattacg attaggcaga acctccgtac caacaatggc attaatggtg
    24601 gttgatttcc ctgctttcat ggtaccgaca attgccagca ccatttccag tcgggaaatt
    24661 ttacacaact cattattcag catcgcgtga cgttcggcga tattaggctg actccagggt
    24721 aaagccagtt gtggcgcgtc gtctccgggt acagagagag gcattttttc cagtaactgc
    24781 aactgttggc gagaaagctg taacaggcgt tcagcctcct gacttaactc atacagggtc
    24841 tgtgtgtaca tagaaaattc ttccttaaag caaattttgt tacttttatt tagccagatt
    24901 gtttttgagt tctgttttcg gcttttataa ttactgcaag aaataatttt atatttagtg
    24961 tgttgttttt tatcagaata aataacgtct tctgatgcgt ttaaaacgtc agaaagataa
    25021 aaatatcatg tgaataaaaa aaagaacaag tagagcatta acattatctt aaataataaa
    25081 tagaggcaaa aagattattt tctttttacg tttcctttca aatgaaaacg atcgtcgtct
    25141 aaaatcagca gtacccccga caaactcagg gattttgtgt ataattgcgg cctttttcgg
    25201 caatctgccg ttttttggcg cttttgccct gctgactttt gaggaaatcc acatgtcatt
    25261 accacactgc ccaaaatgca actccgaata cacttacgaa gataacggca tgtacatctg
    25321 cccggaatgt gcctacgaat ggaacgacgc agaacctgca caggaaagcg acgagctgat
    25381 cgttaaagat gctaacggca atctgctggc tgacggcgac agcgttacca tcattaaaga
    25441 tctgaaggtg aaaggtagct cttcgatgct gaaaattggc accaaagtga aaaacatccg
    25501 cctggttgaa ggcgaccata acatcgattg caaaatcgac ggttttggtc cgatgaaact
    25561 gaaatctgag tttgtgaaaa agaactgatt gtattgtgat cggtaagccg gataaggcgc
    25621 tcgcgccgca tccggcaacg atgccgactg cctgatgcga cgcttgcgcg tcttatcagg
    25681 cagcctctca tctgtataaa tttcgaacta cacttaactg gcttctctta actgaggtca
    25741 ccatcatgcc gttaagtccc tacctctctt ttgccggtaa ctgttccgac gcgattgcct
    25801 attatcaacg tacgttgggc gcggaactgc tctataaaat cagcttcggc gaaatgccga
    25861 aatcagcgca ggacagcgcc gagaactgcc cttccggaat gcaatttccc gataccgcca
    25921 tcgctcatgc caacgtgcgc attgccggaa gcgacatcat gatgagcgat gctataccgt
    25981 caggaaaagc cagctactcc ggctttacgc tggtgctcga ttcgcaacag gtcgaagaag
    26041 gaaaacgctg gtttgataac cttgccgcta acggaaaaat cgaaatggcc tggcaggaaa
    26101 ctttctgggc gcatggcttt ggcaaagtca ccgataaatt tggcgtaccg tggatgatta
    26161 atgtcgtcaa acaacaacca acgcaataac ccgccgggag gcccgccctc ccgcactgtc
    26221 atcgaattcc cgttaactct tcatctgtta gtcactttta attaaccaaa tcgtcacaat
    26281 aatccgccac gatggagcca cttttttagg gaggctgcat catgcaaacg attatccgtg
    26341 tcgagaagct cgccaaaacc ttcaatcagc atcaagcgct gcatgcggtt gatctgaaca
    26401 ttcatcacag tgaaatggtg gctctgcttg ggccgtcggg ttccggcaaa tccacccttt
    26461 tacgtcattt aagcggtttg attaccggcg ataaatccgc cggtagccat atcgagctac
    26521 tgggccgcac tgtccagcgc gaaggccgcc tggcccgcga tatccgcaaa agccgcgcca
    26581 acaccggcta catctttcaa caattcaacc tggtgaaccg cctgagcgta ctggagaacg
    26641 tgctgattgg cgcgctcggc agcacgccgt tctggcgcac ctgttttagc tggttcaccg
    26701 gcgagcagaa acagcgcgcg ttacaggcgc tgacccgcgt tggcatggtg cattttgccc
    26761 atcagcgcgt ttccaccctc tccggcggcc agcagcaacg tgtggcgatt gcccgtgcgc
    26821 tgatgcagca ggcgaaagtg attctggccg atgaacccat cgcctcgctg gacccggaat
    26881 cagcgcgcat cgtgatggac accctgcgcg acatcaacca gaacgacggc atcaccgtgg
    26941 tcgtcacgct gcatcaggtg gattacgccc tgcgctactg cgaacgcatc gtcgccctgc
    27001 gtcaggggca cgtcttctac gacggcagca gccaactgtt tgataacgaa cgttttgacc
    27061 atctctaccg cagcattaac cgcatcgaag agaacgcgaa agctgcctga tatccccatc
    27121 attgaggaaa acgaatgaac gctaagataa ttgcctcgct ggccttcacc agcatgttca
    27181 gcctcagcac cctgttaacc ccggcacacg ccgaagagca ggaaaaggcg ctgaatttcg
    27241 gcattatttc aacggaatca cagcaaaacc tgaaaccgca atggacgccg tttttacagg
    27301 atatggagaa gaaactgggc gtgaaggtca acgccttctt tgccccggac tacgcgggca
    27361 ttatccaggg gatgcgcttc aataaagtgg atattgcctg gtacggcaac ctgtcggcga
    27421 tggaagcggt ggatcgcgcc aatggccagg tcttcgccca gacggtcgcg gcggatggat
    27481 cgccgggtta ctggagcgtg ttgatcgtca acaaagacag cccgatcaac aacctgaacg
    27541 atctgctggc gaagcggaaa gatctcacct tcggcaatgg cgatcctaac tccacctctg
    27601 gcttcctcgt ccccggttac tacgtcttcg ctaaaaacaa tatctccgcc agcgacttca
    27661 agcgcaccgt caacgccggg catgaaacca acgcgctggc cgtcgccaac aagcaagtgg
    27721 atgttgccac caacaacacc gaaaacctcg acaagctgaa aacctccgcg ccggagaagc
    27781 ttaaagagct gaaggtgatc tggaaatcgc cgctgatcct aggcgatccg atcgtctggc
    27841 gcaagaatct gtccgaaact accaaagaca agatctacga cttctttatg aactacggaa
    27901 aaacgccgga agagaaagcg gtgctggaac gcctgggctg ggcgccgttc cgcgcttcca
    27961 gcgacctgca actggtgccg attcgccagc tcgcactgtt taaagagata caaagcgtga
    28021 aaggcaataa agggctgaat gagcaggaca agctggcgaa aaccaccgag attcaggcgc
    28081 agctggatga cctggaccgc ctgaacaacg cgttaagcgc gatgagttcg gtgagtaaag
    28141 cggtgcagta aatcgtaggt cggataagac gccccggcgt cgcatccgac aatgtgtagg
    28201 cgttgatgcc ggatgcggtg caagcacctt atccggccta cagaccggag ccaaacatgc
    28261 aaaccatcac catcgcccca cccaagcgta gctggttctc gcttctgagc tgggccgttg
    28321 ttctcgacgt gctggtcgtc tcgtggcagg gcgcggaaat ggccccgctt acgctgatta
    28381 aagacggcgg caacatggca accttc
//
LOCUS       DAEEUE010000048        28338 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.048, whole genome shotgun sequence.
ACCESSION   DAEEUE010000048 DAEEUE010000000
VERSION     DAEEUE010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28338
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.048"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="KLK84_002214"
                     /pseudo
     CDS             complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="KLK84_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
     gene            complement(998..2020)
                     /locus_tag="KLK84_002215"
     CDS             complement(998..2020)
                     /locus_tag="KLK84_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE2009526.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2034..3536)
                     /locus_tag="KLK84_002216"
     CDS             complement(2034..3536)
                     /locus_tag="KLK84_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBE2009527.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3676..4632)
                     /gene="ytfQ"
                     /locus_tag="KLK84_002217"
                     /pseudo
     CDS             complement(3676..4632)
                     /gene="ytfQ"
                     /locus_tag="KLK84_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4942..5472
                     /gene="ppa"
                     /locus_tag="KLK84_002218"
     CDS             4942..5472
                     /gene="ppa"
                     /locus_tag="KLK84_002218"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HBE2009528.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5851..6192)
                     /locus_tag="KLK84_002219"
     CDS             complement(5851..6192)
                     /locus_tag="KLK84_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HBE2009529.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6195..9974)
                     /gene="tamB"
                     /locus_tag="KLK84_002220"
     CDS             complement(6195..9974)
                     /gene="tamB"
                     /locus_tag="KLK84_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HBE2009530.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGNADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9971..11704)
                     /gene="tamA"
                     /locus_tag="KLK84_002221"
     CDS             complement(9971..11704)
                     /gene="tamA"
                     /locus_tag="KLK84_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HBE2009531.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11910..12548
                     /gene="msrA"
                     /locus_tag="KLK84_002222"
     CDS             11910..12548
                     /gene="msrA"
                     /locus_tag="KLK84_002222"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBE2009532.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12871..14214
                     /locus_tag="KLK84_002223"
     CDS             12871..14214
                     /locus_tag="KLK84_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBE2009533.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14276..14482)
                     /locus_tag="KLK84_002224"
     CDS             complement(14276..14482)
                     /locus_tag="KLK84_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HBE2009534.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14807..15361
                     /locus_tag="KLK84_002225"
     CDS             14807..15361
                     /locus_tag="KLK84_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBE2009535.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15424..16362)
                     /locus_tag="KLK84_002226"
     CDS             complement(15424..16362)
                     /locus_tag="KLK84_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009536.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16574..17314)
                     /gene="cysQ"
                     /locus_tag="KLK84_002227"
     CDS             complement(16574..17314)
                     /gene="cysQ"
                     /locus_tag="KLK84_002227"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HBE2009537.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17504..19447
                     /gene="cpdB"
                     /locus_tag="KLK84_002228"
     CDS             17504..19447
                     /gene="cpdB"
                     /locus_tag="KLK84_002228"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HBE2009538.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19573..19953)
                     /locus_tag="KLK84_002229"
     CDS             complement(19573..19953)
                     /locus_tag="KLK84_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBE2009539.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20042..20902
                     /gene="qorB"
                     /locus_tag="KLK84_002230"
     CDS             20042..20902
                     /gene="qorB"
                     /locus_tag="KLK84_002230"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBE2009540.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            21011..21976
                     /locus_tag="KLK84_002231"
     CDS             21011..21976
                     /locus_tag="KLK84_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBE2009541.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATVQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            22084..22746
                     /gene="ytfE"
                     /locus_tag="KLK84_002232"
     CDS             22084..22746
                     /gene="ytfE"
                     /locus_tag="KLK84_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HBE2009542.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22791..24203)
                     /gene="cycA"
                     /locus_tag="KLK84_002233"
                     /gene_synonym="dagA"
     CDS             complement(22791..24203)
                     /gene="cycA"
                     /locus_tag="KLK84_002233"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HBE2009543.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGSAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24511..25131)
                     /gene="fklB"
                     /locus_tag="KLK84_002234"
     CDS             complement(24511..25131)
                     /gene="fklB"
                     /locus_tag="KLK84_002234"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBE2009544.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25350..25895
                     /locus_tag="KLK84_002235"
     CDS             25350..25895
                     /locus_tag="KLK84_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HBE2009545.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25879..26526)
                     /locus_tag="KLK84_002236"
     CDS             complement(25879..26526)
                     /locus_tag="KLK84_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBE2009546.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26738..27529
                     /locus_tag="KLK84_002237"
     CDS             26738..27529
                     /locus_tag="KLK84_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HBE2009547.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27539..>28338
                     /locus_tag="KLK84_002238"
     CDS             27539..>28338
                     /locus_tag="KLK84_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBE2009548.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDIN"
BASE COUNT         6953 a         7541 c         6989 g         6855 t
ORIGIN      
        1 cacggagagg catttttatc gctgcgcaat gttgactctt gtcactggcg aactctgacg
       61 attctcccac agcaccgtta acccacgctg taatgctata aaaataaaca acaaaatacc
      121 gatggcgatt ttcgtccact aggaactcag cgtgccgtca aagttgatgt aagtctggat
      181 cagtccctga attgccacgc caaaaagcgt cccaagcacc gtcccgacgc caccacttaa
      241 caacgtaccg ccaatcacca ccgacgcgat ggcgtccagt tccacaccta cgcccgccag
      301 tgcatatccg gcttgggtat aaatcgagaa gacaatcccc gccagtgttg ccagtccggt
      361 ggagagcata taaatgcgaa tagtggtgct gcgagtagaa atccccatca ggttcgccga
      421 cgtcgcgttg ccgccaatag cgtacacctg attgccaaaa cgggtacaat gcgcaaggaa
      481 aataccgata accaccaccg ccagcatcaa cagccccatc gcactaaggc gaccgccgcc
      541 ggggattttc cacgcaaggc ttgagagcgt gtcataaatg ggatggttta tcggaatcga
      601 ctcttccgaa acgagatagc tgacgccgcg caaaaagaac atccccgcaa gcgtaatgat
      661 aaatgccggg atcttcaggg cgtcgatcag aagccccata aatgcgccga aggcacagcc
      721 catcaccagc actagcggaa acgccagcag cggcgagagg ccgaaatcgc caaccacttt
      781 tgccagaaac acgccagtaa aggcgatcac cgaaccgacg gagagatcga taccgccaga
      841 gaggatcaca aaggtcatgc caacggcgat tatcccaaga aaggcgttat cggtcaggat
      901 attgcagatc acccttgtgg aagcaaaacc gggaaactgg gtcaggcagt agagatagcc
      961 cagcacaaag acgccgatgg tgatcatcag cggcaagtta cgttttatca tggctacgta
     1021 ctcctttaat cagactgata aagcgttgcg actgaacaat cagcacacaa agcaccacca
     1081 ccgctttcac cacctggttc atctccggcg gaaagcccga aagcaaaatt ccggtgttca
     1141 tcccctgaat aatcagcgcc cccactaccg agagcagcag gttaaagcgc ccgcccatca
     1201 gcgatccgcc gccaatcacc accgcgagaa tggcgtccag ctccagccat aatccggcgt
     1261 tgttggcatc ggcaccgcga atatccgccg ccacgataat gcccgcaatc gccgcacata
     1321 gcccgctcaa cacgtaggtg agcatgacga tgattcgcgt gtttaccccg gcattttttg
     1381 ccgcccgaat gttgatacca acgacttcga taaacatccc cagcgccgtt ttgcgggtca
     1441 acagccagaa caggagaagc gtcagcaccg cgataatgac cggcgttggc aggaacaaca
     1501 gcgatccgct gccaaaccat gagagatccg gcgagttaaa cgtgacgatc tgcccggcgg
     1561 tgatcagttg cgccacgccg cgcccggcga ccatcaggat cagggtggcg acaaacggct
     1621 gaattttgag gatcgctacc agtatgccgt tccacaatcc cgccaggatg ccggtgccca
     1681 gggcgcttaa caaaacaatc ggcaggctga atcccgcgac cgtcatcgca gccgttgtgg
     1741 ctccggcgat agccatcacc gcccccacag agagatcgat accaccggtg gcgatcacca
     1801 gcgtcatacc aatcgccagt aacgcaacgg gagctgcacg gttaagaatg tcgatagggc
     1861 taccgaacaa acgcccatcc tgaagcacca cctgccagaa atgcggggct accaggctat
     1921 cgaccagcag caccagcaac agtgccgcca gctgcggcat ccccgtcggc cagtgaaaac
     1981 gcctttttgg cggcgtagtg tcagggagag attgaggcat cacactgttc tccttacgcc
     2041 gcaatggcgt tcatgatcgc cggaacggaa agctgtgcca gcgggatctc cgccacctgt
     2101 ttgcgatcgc gcatgatgat cacccggtcg gcatagccca ccagctcttc cagttcggag
     2161 gagatcacca gcagcgccag accgtcagca cacagcgttt caatcaggcg gatgatctcg
     2221 gcgtgggcac caacatcaat gccacgggtt agctcatcaa ggatcagaaa ttgcggtcgg
     2281 gtcagtagcc aacgtgaaag cagcactttt tgctgattgc caccggagag aaattcaatc
     2341 ggttgttcag ttgaaggtgt gcgaatgcca agctggcgga taaagcgttc ggcaatctct
     2401 tgctgctctt tgcgggaaat gggtcgtagc cagccgcgct gggcctggag agcgaggatg
     2461 atattttccc gcaccgaggc ggcggcgatg atgccatcgg ttttcctgtc ttccgggcag
     2521 aatccgatgc ccagtactga agcctgatgt ggcgatcgca gggtttgcgg tttgcctttg
     2581 atcaacgccg tgccgctgtc agcaggtttg ataccgaaga tcacttcggc ggtttcggta
     2641 cgtccggatc ccagcaagcc agccagaccg acgatctcgc cggggcgcac ttcgagatca
     2701 aacggtgcta tcgttccttt tttgccgtaa tttttgaacg cggcaacggg tttgtcgctc
     2761 aacaatgttc gcccggcacg ctgtagcgcg tgggtatcca gctcgcgccc cagcatcatt
     2821 tttaccagtt cgatctgcgg tagctcgcgc gtttcccgac agcctacgaa actgccgttg
     2881 cgtaagacgg tgatccgatc gctgacctga tagacctgat cgagaaagtg agtgacaaag
     2941 atcaggctga cgccgcgatc gcgcaactga cgcatcaggc caaataatag ctccatttcc
     3001 tgggtgtcga ggctggcggt gggttcatcg aggatcagca ctttggcgga gagatcgata
     3061 gcccggcaaa tggcgacgat ttgctgcatc gccaccgaaa aacggttaag cggttcgcgc
     3121 acgtcgaggg agaaaccgta agatgccatc agttcggtgg cgcgcttttc catctcttta
     3181 cggcgtagaa ggccgaaacg tttgggttcg cgacctatga atagattatc agcaaccgac
     3241 atattgggta gcaggttgac ttcctgatag acggtgccga taccgagttg ttgtgcatgg
     3301 gcggtatttt tcggtgagat agtctggcct tccagccaga tggtgccgcg atcggcgtgg
     3361 tatacaccag ttaatgcttt gattagcgtt gattttcccg ccccgttttc accaagcagc
     3421 gccatgattt cgccacggcg caggctgaaa tcaacgttgt ctaacgcttt gacgccgggg
     3481 aaaaatttac ttaatccttc ggtgcggagg atctcctggt gttggtcggt cgtcatggtt
     3541 tcccccggtg taaagcgtgc tcccagtagg catgataaga cgcggtaagc gtcgcatcag
     3601 gcttcagtgc accatcgccg gatgcggcgc gagcgcctta tccggcctac gaatcggcat
     3661 caggcatagc aaccctcaat accccatatt tttcttcttc tctaactctt cttttgcggt
     3721 atcaggcagg taaagggtgg atttggttaa cgtcagcttt tcaggcatgg tgccgtcttt
     3781 tttgtatttc tccagcgcat cgaaagcggg tcccgccatg ttcggcgtca gttcaacact
     3841 ggcgttcgct tcgccatcca tcatcgcttt gtagatgtcc ggtacaccgt caatggaacc
     3901 cgtgaggata tctttgcccg gtttcaggcc cgcttcttta attgcctgaa ttgcaccaat
     3961 caccatgtcg tcgttatggg cgtaaaccat gcagatgttt ttgctgttgt tttccgcttt
     4021 gataaagctc tccatgactt ctttgccttt actgcgggtg aagtcacctg actgcgagcg
     4081 gataattttg atatttggcg cattcttaat ggcttcggca aagcctttct tacggtcaat
     4141 ggcgacgctg gccccaacgg tgccctgcag ctccaccacg ttgcatggtt tgccattcac
     4201 ttcttttacc agccagtcac cgatcaactt accttcgagg atgttgtcgg cagtaacggt
     4261 ggtcatatag agagatttgt ctttcacatc gatggaacgg tcgagcaaga agaccgggat
     4321 ttcggcatct ttcgcctctt ttaataccgg ttcccaaccc gtcgcaacca ctggagcgat
     4381 aaagatcgca tccacccctt atgcaacgaa ggaacgtacc gctttaatct ggttttcctg
     4441 cttttgctga ccatcggcaa ttttcaacgt aattccgcgt ttttcggcct cacttttcgc
     4501 cacattggtt tctgcggcgc gccagccgga ttccgatccg acctgcgaaa atcctacggt
     4561 taatggagcg gccaacgcca tagacgacat ggctgccgag actgcagaga ctacaagtaa
     4621 gcgtttccac atagggttgt cctcgtcggg gtaattatta ttggtgaaaa gatgttcgcg
     4681 aaaaaactat agacaattcg ttatgtaacg gattgcgtta catcacactt caaaaaagta
     4741 aataaaacgt taatcacaag tttgtaatcg ctttcatctc actatgaaaa atgcggctac
     4801 ggttatggat tttcctgctc tgtataccgt cttaaaactg gcgaaaaagg aaaatgaaga
     4861 cgaaaacaag caaagacatt cggcgcgagt tggctataat acttggcact tgtttgccac
     4921 atatttttaa aggaaacaga catgagctta ctcaacgtcc ctgcgggtaa agatctgccg
     4981 gaagacatct acgttgttat tgagatcccg gctaacgcag atccgatcaa atacgaaatc
     5041 gacaaagaga gcggcgcact gttcgttgac cgcttcatgt ccaccgcgat gttctatccg
     5101 tgcaactacg gttacatcaa ccacaccctg tctctggacg gtgacccggt tgacgtactg
     5161 gtcccgactc cgtacccgct gcagcctggt tctgtgatcc gttgccgtcc ggttgccgtt
     5221 ctgaaaatga ccgacgaagc cggtgaagat gcgaaactgg ttgcggttcc gcacagcaag
     5281 ctgagcaaag aatacgatca cattaaagac gtaaacgatc tgcctgaact gctgaaagcg
     5341 caaatcgctc acttcttcga gcactacaaa gacctcgaaa aaggcaagtg ggtgaaagtt
     5401 gaaggttggg aaaacgcaga agccgctaaa gctgaaatcg ttgcttcctt cgagcgcgca
     5461 aagaataaat aagttcttct agcgcaataa ccctgaacgc cgggcttcgg ttagtaaggg
     5521 tttttttatg cccgcgataa ataaactctc tattccacca tcattattct cagcggttgc
     5581 aaggcttgaa cggtaagaac aagcaaaccc gaccaccatt ttgctgttca tagccacttg
     5641 ctggaagtta gccgacctca ctcatactca ccgttcaaag cgtgcccccg ttttacccct
     5701 gcacatttgt gaacgccagc agagtttttt atgcgattgc agatatggaa agcggctcgg
     5761 agagaaaagc aaaagatgga aaagttacaa aaaaaatcgc gagatacatc gcataaaaca
     5821 acgccacccg aaggaggcgt gcttttatta ttacttatcc ctgtctaacc agtcgccgct
     5881 ttcaattagc tttaatccat cgacgggtcg ttgataaacg tacatccatg cactcccata
     5941 cggcgtctga atcaactggc gcgcgtattc accgcccctg gtgcgcaagg catcaagttc
     6001 ggccagcgtg gcgttgtcaa tacgataaac ttcaccgtgt accgttccgt tccccggaac
     6061 tgcgcctgga tagtggccca ggctatacaa ctggtagtta tcgatactga aatcgcccag
     6121 taactgggca ttggtcatcc agtgactgtt gccttgtttg tggcgtaaac tgccgtagac
     6181 aaatattcgc attgctaaaa ctcgaactga tagagcaaat ccagtgcctg gtctacacca
     6241 gacacggctt ccagatatag cttaggcatc aggcgataac gtaacgtgag tgttgctata
     6301 gagtcaaata tacccacgcc gtatttcact tgcagacctg gcaatacata gccgctgacc
     6361 actacctggg aggagtcgcc tactcccagg gtgtcgagcg ctaaattgct tacgccaaac
     6421 gtctcgccga ttttacccac aatctggcca ctttgcgcaa cccccagacc aatcagcatc
     6481 gaggtcattg ccgcactgtc gctctgatcg ctctccagcc cttgtccacg tagcaaataa
     6541 gacaatgcag cttgttgcga catcgccggg tcagagaaga tctccgcttt cggttcgtcc
     6601 gccagaccag tgacgcgaac tccggcgatt acatcgtctt ctgtagcatc cgggttacga
     6661 atagcttcaa tattaagata cggttgatcc ggcggaccag agaacagtaa ctcaccctta
     6721 cgcacaatca gatcctgacc ataggcatgg aagcgccctt cagggatgtt gatctgcccg
     6781 ttcaggccca gcccttgttt gtcctgtacg acgttaagat cgcccgtcag ccgcgctttc
     6841 aggccaaagg cgtcaatgcg cacattgttg ccaacgtgga caatcaggtt actgttaatc
     6901 ggaatcgacg ccgttttcgg ctcttccggt tgcaggttat cgttaagcat caccacatcg
     6961 ctggagacgc ctactgcgct ttccggcaga tcgtgcacca cgatgcgcgc ccacgggaca
     7021 tccacgcgac catcgagggt aaacaggttt ggtgtagcct cgaatacaac atctggcgat
     7081 acatccattc gtaccatcgg cggcacggtg atccgcactt tactgccttt cgccgttact
     7141 cgtgcccgcc agttttcaat ttggctccag tcggcattac cgttcaggta gatttcaccc
     7201 tgctgggtcc gtactgtacc ggcaagcgtc gagcgcatac cgttaaagtt gaccgcaagc
     7261 tggctcggct gcatatcaaa cggcataaag ttgccgtcga tatccacacc cgtaacctga
     7321 agctgaccaa acaactgcgg gctttgcaca tcaccaccca gacgcaagtt ggcactcacc
     7381 atccccgctg ctttttcccc acgggtaaag atggggttta tcatcgcaag gttgaagtta
     7441 cggatattga cgttgccacc aagattacgg cggccttgcg gatcggtcac ctgcacctgt
     7501 ccatcaaact ggccgttatt ggtcaggcgg atggtccagc ccaattcggc acggttgtta
     7561 cgcaattccg ccgtcagatt cagtgtctga aacgccaccg gcagcgccgc atcgttgacg
     7621 gtttgcgtta cctgcacgtt acgccccgaa agggtgatac tgccctgcgg cagcccctct
     7681 ttcgtggtgt cccaggcaac atccgcttta cccgtgaaga taccgctggc ctgagtggtt
     7741 tctggcataa acggtttcag catggcgagg tcgaagcggt tgagattcac caccgcacgc
     7801 ccttcggccc ccgcatcgat agtttgcggc acgcacagtt ccgcattcgg gttaagccag
     7861 cagtgtggcc cgatgctgat tttttgctcc ttattgcggt aatccagcgc aatatcgcgg
     7921 gtcagcgacc acgggccgac cggcgtctgg aagcgggtat tgctaagagt tcccttccag
     7981 cgttcttctt tgcgatcaaa acttcctgcc agattaagct gcccggagac aggctcgccc
     8041 tgaatccgca actgtagctc gtgctgcttt tcgctgcctt tggcattcag ggtgacgagg
     8101 ttgatattta catccggctg cgaaatttgc tcaacgcgta cgtcgagttt cccggcgatc
     8161 tgatcggtgg atttaatgtc gccttccacg cgaacctgcg ccacggaaag ttcctgccag
     8221 cgcaggccgc gcgcggtgat atctgccagt agttgtggcg cttccaccgt gccgcgtact
     8281 ttcaccagcc ctttcgctgt accgccaagc cccggcagcg cgttatccag ccccggcgcg
     8341 ttgatggtgg catcaagatt gagatctttt acccccagct cgcctttcac ttcggcactg
     8401 tttggcccga gttccagatg aagcccaggg atcatccact gcatataact gttgcctttc
     8461 agcgtgccgt caacgttcac tttgttctgt ttgacgttac cggtcagctt cagttctggc
     8521 acctccatct gccaggtgcc gccgtacagg ctaccgcggg ttttaatcaa gccattgagt
     8581 ttcgacggcc actccgggaa ctctttggcg gtgttaatgc cattaagcgt tagctcaccg
     8641 cgccaactaa tggcctgctg ccagtcgagc aacgccttga gttcagtttt cccttccagc
     8701 gccgcgacgg tgagtttgtc gagattgacc tgctgttcat tacctttggc atcaagggta
     8761 atggtggctg gcgggatctc ctgtcccttc actgccgtac gcatagagag cgtgtaatcg
     8821 gtcattttgc cggtaagttt cagtttcaga tcatccgcct gatactgctt ctcaccagtg
     8881 aacggccagt aaagctgttt gctgttcact tccacgttga gtggcaatcc ggcttccgcc
     8941 agtcgcgtct gggcgcgtaa atccatatcc accggaccgg aaaggttaac gcctatctcc
     9001 agctgttcgc gcagcgcgcc gcccactttc agcttcacct tttcaccttt caacggctcc
     9061 acgttcagtg tgctgttgag ggtgatatcc accggccagt tgtctgacag ctgcgccgtg
     9121 ccgctggcgt tgacgatccc ctggttggaa tcgatatcca gggcgtccag tttagtattg
     9181 ccgtcaatgc tgctcacttt cagcagcatg gtgcgcacgg tgatgtccgt gtcgcccgtc
     9241 acgcgcagct gctcgccctt aaactcttca atgttcaggt taagcggcaa atgcacgtcg
     9301 gtcatttccg gcaataccgg gcgagaaaag agatctttca gcgtttcgcc gagcggcttt
     9361 tcctccggct gcggattttc aattttcggt tcgaccactt cttcctgcgc cacttccacc
     9421 actttcggca gagcaatcag caggcctttc agcgacgtcg gtttcagggt cagggttttc
     9481 tcctgccagt tcaggccgga ggtgaagtcc atcaccgaca ccgtggtgtc atcaatcttg
     9541 atgttgacgt tgtccagcgc cacccgtgtc agggtgatgg gatacggcgt ggagagatcc
     9601 agcggaccgc tatcttcctc ttcttcaacc tgttcagaag gaggcatttt tttactgtcg
     9661 atgttgacct gaatgtcttt cagcgccagg tcattaatac aaacgctact gttccacagg
     9721 cactcaagac cgacccccag atgcagatta cccgctttta ccgccacgcc tagctgctca
     9781 taacgaacgt cagacaaggt gagatcgcgc cagccgccgg tgaccttgcc aatatccagt
     9841 cctggcaccc agcgatccgc cgctttaaat acaagatgca ggccgctggt ggtgcccacc
     9901 agaaacgcca ccgatcccag caacagtaag ataacgataa ccacgccgag gctgattttt
     9961 ttccataaac tcataattct ggccccagac cgatgtaaaa ctgtaacccg tgttcgtctt
    10021 tatccgcgac cggtacggca aaatcgagtt tgattggccc gaccggcgat tcccagcgta
    10081 cgccgacccc ggtaccggtt ttaaagtcgc tgcggcgaat atcgcttacc gcttcgccac
    10141 tatcgacaaa caccgcgccc caccattttc cggtcacgtt gtactggtat tccagcgatc
    10201 cggttatcaa cttcgaggcc cctttcaggt caccgttggc gtatttcggg gcgatagatt
    10261 tgtatttgta gccgcgaata ctgcgatcgc ccccgacgaa gaaacgcaga tccggcggta
    10321 ctttgtcgaa atcaccggtt tcaatccagc ccagcgtgcc gcgtgtcaca aagcgatggc
    10381 gatcgtacag tgtgcggatc cagacgtgct gcgcctggaa aacggagaaa tcgacatctg
    10441 aaccccaggc cgtgttggag tagtcgatag agtagcgttg cgagtctccc caggttggca
    10501 tcaggccacc acgagaacgc gtgcggctaa tcatcacccc aggataaaac agcatcgtgg
    10561 tgttggtaat ttcaccctgg gtaaagtggt cgaggctcca gcgcaggtta atggcacgct
    10621 gccagccgct ggagagatcc cagtagcgag aagccaccaa cgtagtggag tcagattcgg
    10681 tatcgttcag gtcagtgcgc ttaaaaccgc cctgcaccaa ataatattgt tccagtggat
    10741 tcttcagcag cggcatttta tagctgaagt cgagggtctg ttccggcgcg gaaatactgg
    10801 tactggtggt caaactgtga ccgtatgagt tcatccacgg ctttttccac gtcgctttca
    10861 cgcgcggtcc cacgtccgta gagtaaccga ccccggtttc gatggtgttt tctgttcgcg
    10921 gcgaaaccac gcccgtcaat ggtaatactt tcgtttcgcg cgctttatca aattgtggag
    10981 ccaccaccac cgagttaaac cagccggtag cagaaagtcg gcggttcagt tccgccagat
    11041 ctttcgattc atactcatcg ccctctttaa acggcaccag attttgcagg tattcatcgc
    11101 ggatttgtga tccttcaaag gtcacatgcc caaagcggta acgttcgcca ctgttataat
    11161 caatatccca gaaggcttta tgcaggccga gcgcaatgcc cagctgcgct ttggtaaatt
    11221 cgctatcgaa ataaccttta cgcaacgcaa tactggttaa ggactttttg aaattttcat
    11281 aatcgccctg gttcagtacc gtaccaatag ccgggcgagt atcgagcaat ttcaaatagt
    11341 ctttatcggt ccgcgcgccg ccgcgcaata ccacatcggt gccgccaatt aacaccggca
    11401 cacctggcgt gactttggcg atcaatacct gccgcccttt ctttggcggt ggacggagat
    11461 caaattcaat ggtcggctgg taataaccca gcgctttcag accttcacgg atggcatcat
    11521 cgacgcgtgc gcgaaagcga cggtctggcg tcacttcatc actttcaatc gtagaaagct
    11581 gcgcacgaac gttcttttcc agctgtcccg ataacccctc gacctgtaga cggacgttcg
    11641 cggcgacggc agatccgctt aagcagagta agcttacaca gcataactgt cggatatagc
    11701 gcacattttc tcctgaatat ccttttttcc tgcccctgga acgccgttaa acggcttaac
    11761 aaaaatccag taatatggat taaaaagcag actaaacccc aaatatttct tatgttttac
    11821 tttagaccta ttcacggtgg ttattgtgtg caaatacgcc tcttgttaca accttgaccc
    11881 caatgaccga ttttcgggag agcgacacca tgagtttatt tgataaaaag catctggttt
    11941 cccccgccga tgccctgcct ggacgtaaca ccccgatgcc cgtagccacg ctgcatgcgg
    12001 tcaacggtca ctcaatgacc aatgtacctg acggaatgga gattgccatt tttgcgatgg
    12061 gttgtttctg gggtgtggag cgtctgttct ggcagttacc cggcgtttac agcaccgccg
    12121 caggctatac cggcggctat acgccaaatc cgacttatcg ggaagtgtgc tccggtgata
    12181 cgggtcatgc cgaagcggta cgcattgttt acgatccttc cgtcatcagc tatgagcagt
    12241 tgctacaggt attttgggag aatcacgatc ccgcccaggg aatgcgtcag ggcaatgacc
    12301 acggcacgca gtatcgttca gcgatttatc cgctgacccc agaacaggat gccgcagctc
    12361 gcgccagtct ggaacgtttt caggcggcga tgcttgccgc cgatgacgat cgtcacatca
    12421 ccacggaaat cgctaacgcc acaccgtttt attatgccga agatgaccac cagcaatatc
    12481 tgcataaaaa cccatatggt tactgtggaa ttggcggaat tggcgtctgt ctgccgccgg
    12541 aagcatagcg ttacgggtac aaatgtagtt tgttgataaa gtgctctttg tctatgcctg
    12601 atgcggctta aacgacttac caggcctaca aaatcgccca aattcaatat attgcaagga
    12661 ctgcgtaggc ctgatcggca tagcgcatca ggcagtattg catttatcag cagtctgaat
    12721 tttaaccctc tggcgacttt acagtacctt acgctatact agccactgaa aatgccggtt
    12781 cactttcttc gaatcggctt tcaatgtgta tttcacacaa attaatcaac tttcccttcc
    12841 gagggtctgg cctgaaaggt cggataagat atgttaaaca gtattttagt catactctgc
    12901 ttgatcgctg taagtgcgtt cttctcgatg tccgagatct cgcttgccgc ctcacgcaaa
    12961 atcaaactta aactgctggc tgatgaaggc aatataaatg cccaacgcgt tctgaatatg
    13021 caggaaaatc ccggcatgtt ctttaccgtg gtccaaatcg gtctgaacgc agtggcgatt
    13081 ctcggcggta tcgtcggtga tgcggcattt tctccagctt ttcacagcct gttctcccgc
    13141 tatatgtcgg cagagctctc tgagcaactg agctttattc tctctttctc gttagtgact
    13201 ggcatgttta tcctgtttgc ggatttaacc ccgaaacgca tcggtatgat tgcgccagaa
    13261 gcggtggctt tgcgtatcat caacccgatg cgcttctgcc tgtacgtttg caccccgctg
    13321 gtgtggttct tcaacggcct ggcgaacatg atcttccgta ttttcaaact gccaatggta
    13381 cgtaaagatg acatcacttc tgatgacatc tacgcggtag tggaagccgg tgcgctggcg
    13441 ggcgtgttac gtaaacagga acacgagctg attgaaaacg tctttgagct ggaatcccgt
    13501 accgttccgt cttcaatgac accgcgtgaa aacgtgattt ggtttgatct ccacgaagat
    13561 gagcaaagcc tgaagaataa ggtggcggaa catccgcact ctaagttcct cgtctgtaat
    13621 gaagatattg accacatcat cggttatgtc gattctaaag acctgctgaa ccgcgtgctg
    13681 gctaaccaaa gcctggcact gaacagcggc gtacaaattc gcaacacgct gattgtgccg
    13741 gatacgttaa ccctttcaga ggcgttggaa agttttaaaa ccgcaggtga agacttcgcg
    13801 gtgatcatga acgagtacgc gctggtggtg gggatcatca ccctcaacga cgtgatgacc
    13861 acgctgatgg gcgatctggt cggtcagggg ctggaagagc agattgtcgc ccgtgatgag
    13921 aactcatggc tgattgacgg cggcacccca attgacgacg tcatgcgcgt gctggatatt
    13981 gacgagttcc cgcagtcggg caactacgaa accatcggtg gctttatgat gtttatgctg
    14041 cgtaagatcc cgaaacgcac cgattcggtg aaattcgccg gctacaaatt tgaagtggtg
    14101 gatatcgata actaccgcat cgaccagctg ctggtgaccc ggatcgacag caaggccacc
    14161 gccctttcgc caaaactgcc tgacgctaaa gataaagaag aaagcgtcgc gtaaccccca
    14221 gaaacatcaa cggctcctga atcaggagcc gtcttattac tgcatagcac tttggttaag
    14281 ccatctctgt ttgcagacgc ataacctgac ggttaacttc ggacatcact gacaaatgca
    14341 gcttatcctt cacttttggg ataagaatct tacccttatc aaattcaaaa gcgccaatgt
    14401 ccttgatgta taaccgtcca cggaacagga tctttacgta cttcgccacc tgaagtgggt
    14461 tgtaccgttg gaaaattttc attgttatct cctgctgagt attacgcctg tgcggtgcca
    14521 caatcggccc aactattatg aggcgcaaat tttaatgctt agtgactata gactatccgg
    14581 gcaatgtttc caccgcgtat aacttttttt acctaaaggt tacaattatt cagaattatc
    14641 tttttaccga agcgcggtct tcagtataag cattcatttt tcatatgatt tgtgcgcttg
    14701 accgcaaact ggcatcacac ttgcgggaaa ttcgataaat agcacatatg attaaaactc
    14761 agacccaagt ggtcggatca cctgcatatc ataagaagga aacaccatga ccctacgcaa
    14821 gattctggca ctcacctgcc tgctgttgcc gatgatggct tccgcacatc agttcgaaat
    14881 cggtcagcga gtgccgccga ttggcattac cgatcggggc gagttggtgc ttgataaaga
    14941 tcagtttagc tacaaaacct ggaacagcgc gcagttagtg ggaaaagtgc gagtactgca
    15001 acatattgct ggtcgcacct ctgcaaaaga gaaaaacgcg acgctgattg aagcgattaa
    15061 atcagcgaag ttaccgcacg atcgttacca gaccaccacc attgttaaca ccgacgacgc
    15121 gattccgggt tccggcatgt ttgtgcgcag cagtctggag agtaataaaa agctttatcc
    15181 ctggtcgcag tttattgtcg atagcaatgg cgtcgcgcgc ggtgcctggc agctggatga
    15241 agaaagttcc gctgtagtgg tgctggataa agacggtcgc gtgcaatggg ccaaagacgg
    15301 ggcgctcaca ccggaagagg tgcagcaagt gatggacctg ctgcaaaagt tgctcaaata
    15361 atggcaataa cataaattct ggaaaaataa ttttccccta tcgttatcta tgggataggg
    15421 gaattaagtc tctgagttct tacaccgaac taatactggt aacgctagtt gctctttttt
    15481 aaacgcctca atggtcgatt tatatttctc gcaaatcttt tctttggtaa tattactatc
    15541 tgcggccaga ttcgcactta ctatcgcatc cacttgttcg gctaattcga cttgttggca
    15601 acctgcatta accagaaaat tataaataag agagtaatct tcatcctcca aaacaacaga
    15661 ggaaccatta cgaaaacgtt catcccctcc atagatttta agcgcaaagc ccaaagattc
    15721 tatgactatg gcaaaattat catcaattat tttttcgtaa atataccatc catcgcattc
    15781 tttaattcca ccgcctggta tttggagttc aaatttaccc cgttttaatt tgcttactac
    15841 atcctccaat aaggcataac cgaaagatac gtcaccaaac gcctggcgaa tataaccttt
    15901 aatccttttt ggtatgtcag actgtttttt ctgcaaaata tcacaggcat acaatcttct
    15961 gttttgttct aacggaaact cgcggttttt gaaatccatg aatgagttat actcatttat
    16021 tgttatggga atttcttcgt attcaaaccc actgcctcgc aatgttattg tatattcagc
    16081 accgttatct ttaaggctga aactgataag aaaattatcc atggcattaa cagtttcatt
    16141 ttttgatgaa agcacttgat aactattttt atcaatctgt gttgcgcaac gaaaggactc
    16201 ccgcaaagtt aacagactat cataattcga aacccaatct tttttcttaa atgcatgctg
    16261 aatattatta aaaaaatcgt ttgaatgtgt ttttgtatgc gatgacttta caaataactc
    16321 caccgcacca ataaaattat tgtgtctgat cctgggtaac atatggctac cttattttat
    16381 ttggttattt tatgtcacca ttaaatatct cattatagaa gtaaactatc aaaaaacgct
    16441 cattattaaa atgtaactta ctagcaattg aatttttgca ctttaaatac aatctctttt
    16501 tatttataac tttttaggtt gggataaatc aggatggcag aatcaggaaa tacactgttt
    16561 ctgccatctg aatttagtaa atggacactc tgaaccccgg attcaggaac gactcgcgcg
    16621 gagtgtaatc cagcggttta ccctgccagt cgtgaacgtg cgctccggca gctgcagcca
    16681 cagcatgtcc ggcggcggtg tcccaaatat tcgttggtcc gaagcgcggg tacagctgcg
    16741 cctgtccttc cgccaccagg cagaatttca gcgaagagcc gatggacgtg gtctgatgtt
    16801 cgccaagctg ttgcagatac tctttcagct ccgcatctgc atgggaacgg ctgataacca
    16861 ccagcggcgg gcgcgcatcg cggacctgaa tcagcttgcg cacaccgcac tcttctttcc
    16921 aggccttgcc ttctgccgcg ctgtacatta cgttcattac cggcgcatac accacaccta
    16981 aaatcggttt gccatggtca atgagcgcaa tgttaacggt gaattcgcca ttacgtttaa
    17041 taaactcttt agtaccatcc agcgggtcta ccagccagta acgctgccag tgctgacgga
    17101 cttcccaacc gggaggatct tcttcagaaa ggaccggaat atccggtgcc agcgtacgta
    17161 aaccgtccat gataacggtg tgagcggcaa tatccgctgc cgttaccgga gaattgtccg
    17221 ctttgctgac gacgtccatc ggtttcgtcc cgtcgtagac ctgcataatg gcatcgcctg
    17281 cattccgtgc aagctggcat acttgatcta acatttctcc acctcgtctc tgtgagcggt
    17341 gttaacttat tgttttactt ataccctatc gttaatgaat gcgccaactg tgatagtgtc
    17401 atcattttca aagcgtaaaa ttgtggcatt cttcactgtt ctataagtaa ggcgtttatt
    17461 ctcccttttc tttcgtattc ccgatgataa aaggatgtcc ctgatgatta agtttagcgc
    17521 aacgctcctg gccacgctga ttgccgccag tgtgaatgca gcgacggtcg atctgcgtat
    17581 catggaaacc actgatctgc atagcaacat gatggatttc gattattaca aagacaccgc
    17641 cacggaaaaa ttcggactgg tacgtacggc aagcctgatt aacgatgccc gcaatgaagt
    17701 gaaaaacagc gtactggtcg ataacggcga tttgattcag gggagtccgc tggccgatta
    17761 catgtcggcg aaaggattaa aagcaggtga tgttcatccg gtttataagg cgctgaatac
    17821 gctggattat acggtcggta cactcggcaa tcatgaattt aactacggtc tggattacct
    17881 gaaaaatgcg ctggcgggag cgaaattccc ttatgtaaat gccaacgtca ttgacgccag
    17941 aaccaaacag ccaatgttta caccgtattt aattaaagat accgaagtgg tcgataaaga
    18001 cggaaaaaaa cagacgctga agattggcta tattggcgtc gtgccgccgc aaatcatggg
    18061 ctgggataaa gctaatttat ccggaaaagt gacggtgaat gatattaccg aaaccgtgcg
    18121 caaatacgtg cctgaaatgc gcgagaaagg tgccgatgtc attgtcgttc tggcgcattc
    18181 cgggctgtct gccgatccgt ataaagtaat ggcggaaaac tcagtttatt acctcagtga
    18241 aattccgggc gttaacgcca ttatgtttgg ccatgctcac gccgttttcc cgggtaaaga
    18301 ttttgctgat atcgaagggg ctgatatcac caaaggcacg ctgaatggtg ttccggcggt
    18361 aatgccgggc atgtggggcg atcatcttgg ggtggtcgac ttacaactca gtaatgacag
    18421 cggtaaatgg caggtgacgc aggcgaaagc ggaagcacgg ccgatttacg acatcgccaa
    18481 taaaaaatcc ctcgcggcgg aagacagcaa gctggtagaa acactcaaag ccgatcacga
    18541 tgccacacgc cagttcgtca gcaagccaat cggtaaatct gccgacaata tgtatagcta
    18601 tctggcgctg gtgcaggacg atccgaccgt gcaagtcgtg aacaacgcgc aaaaagcgta
    18661 tgtcgaacat tacattcagg gcgatccgga tctggcaaaa ctgccggtgc tttcagctgc
    18721 cgcaccgttt aaagttggtg gtcgcaaaaa tgacccggca agctatgtgg aggtggaaaa
    18781 aggccagttg accttccgta atgccgccga tctttatctc taccccaata cgctgattgt
    18841 agtgaaagcc agcggtaaag aggtgaaaga gtggctggag tgctccgccg gacagtttaa
    18901 ccagattgct cctaacagca cgaaaccaca gtcactcatc aactgggatg gtttccgcac
    18961 ttataacttt gatgttattg atggtgtgaa ttatcagatt gatgttaccc aacccgcccg
    19021 ctatgacggc gagtgccaga tgattaatgc caatgcagag cggattaaga acctgacctt
    19081 taatggcaag ccgattgatc cgaacgctat gttcctggtt gccaccaata actatcgcgc
    19141 ttacggcggc aaatttgccg gtacgggcga cagccatatc gcttttgctt caccggatga
    19201 gaaccgctcg gtgctggcag cgtggattgc tgatgagtcg aaacgtgcgg gggaaattca
    19261 tccggcggca gataacaact ggcgtttagc accgatagct gccgataaga aactggatat
    19321 ccgtttcgaa acttccccgt cagataaagc cgcagcgttt attaaagaga aagggcagta
    19381 tccgatgaat aaagtcgcga ccgatgatat cgggtttgcg atttatcagg tggatttgag
    19441 taagtaaaac acttcttttt cggcctataa atcaaccgca tccggcattt attggcggat
    19501 gcgatgctgc cgtatcttat ccaccctaca agccatgaac cgtagaccgg ataagcgcag
    19561 cgcatccggc agttatgccg cacgttcatc ccgcaccgcc aacacctcgg gcaaattcaa
    19621 ctcaatccag tccgccagtg cagcaacctt ttcgctcacc tgctcgccca gcggcgtgag
    19681 gctatattcc acatgcggcg gcaccaccgg atacgcgata cggttaagaa aaccatcctg
    19741 ttctaacgcc tgtaacgact gcgcaagcat cttttcactc accccaccga ttttgcgccg
    19801 caggtcgcta aagcgatgag taccttcgcg tagcgccacc agaatcaaca ccccccaacg
    19861 gctggtgacg tgtttcaaca cctcgcgcga cgggcactgt tccgcaaaga gattaccctc
    19921 tttcagttgt tgtgacaggc taacctgact catttcatac ttaccttttt gcacgtactt
    19981 actaaaagta agtttaggtg ttagcgtatt taaacacaag acaaaacgat ggagacttcc
    20041 catgatcgct attactggtg ccactggcca acttggtcac tatattattg aatccttgat
    20101 gaaaacggtt cctgccagcc aaatagtggc tatcgttcgt aatccggcaa aagcccaggc
    20161 cctggcagca caaggcatta ccgtgcgtca ggctgactac agcgatgaag ccgcactgac
    20221 atctgcactt cagggagtgg aaaaactact gctgatctct tccagcgaag tgggtcaacg
    20281 tgccccgcag catcgtaatg ttattaatgc cgcaaaggcg gctggcgtga aatttatcgc
    20341 ttataccagc ctgctacatg cagatacctc cccgctcggc ctcgccgatg agcacatcga
    20401 gacggagaaa atgctggctg attctggcat cgtttacacc ctgctgcgca acggctggta
    20461 cagcgaaaac tacctcgcca gcgccccggc agcactggaa cacggcgtat ttatcggtgc
    20521 ggcgggcgat ggcaaaatcg cctcagcaac gcgggcagat tatgcgacag ctgcggcacg
    20581 cgtgattagc gaagccggtc acgaaggcaa ggtttacgaa ctggcgggcg atagtgcctg
    20641 gacgttgaca cagttagcgg cagagctgac caaacagagc ggcaaacagg ttacctatca
    20701 aaatctgagc gaagccgatt tcgccgcggc actgaaaagc gtcggactgc ccgacggact
    20761 ggcggatatg ctggcggatt ctgacgttgg cgcatcgaaa ggtggtctgt ttgataacag
    20821 caaaacgctt agcaaattga ttggctgccc aacgacaacg ttagccgaaa gcgtaagcca
    20881 tctttttaat gttaataact agttaattaa agtggcatcc tcccgcatcc tctctgataa
    20941 tgacgggatg ccgggagcaa tcatgtctgc ttcctgaacg tttcttctga cagaccaatg
    21001 gatgccagta atgattagcg gcgtgctgta cgccctgtta gcagggttga tgtgggggct
    21061 tatttttgtc gggccgttga tcgtgccgga atacccggcg atgttgcagt cgatggggcg
    21121 ttatctggcg ttagggttaa ttgcgctacc cattgcctgg ctgggacgcg tgcgtctgcg
    21181 tcagttggcg cgtcgggact ggcttaccgc cttgatgctc actatgatgg gcaacctcat
    21241 ttattacttc tgccttgcca gtgccattca acgtactggc gcgcctgttt ccacgatgat
    21301 tatcggcacc ctgccggtgg tgattcctgt ctttgccaat ctgctttata gccagcgcga
    21361 cggcaaactc gcgtggggaa aactcgcccc ggcactgatt tgtattggca tcggcctggc
    21421 gagtgtgaat attgctgagt taaaccacgg actccccgat tttgactggg cacgttatac
    21481 ctctggcatc gtgctagcgt tagtttccgt ggtctgctgg gcatggtatg ccctgcgcaa
    21541 cgcccgctgg ctgcgggaaa atcccgacaa acatccgatg atgtgggcga cggtgcaggc
    21601 gctggtcacg ctgccggttt ctctcatcgg ctatctcgtc gcctgttact ggctgaatat
    21661 acaaacgccg gacttctcct taccttttgg cccccgtccg ctggtgttta ttagtctgat
    21721 ggttgcgata gccgtgcttt gctcatgggt tggcgcactc tgctggaacg tcgccagcca
    21781 gcgattaccg acagtgattc tcgggccgct gattgttttc gaaacgctgg caggtttgct
    21841 gtacaccttt ttactccgcc agcaaatgcc gccgctaatg acgctgagcg gtatcgcgct
    21901 gttagtgatt ggcgtggtca tcgcggtcag agcaaaacca gaaaaacctt taactgaatc
    21961 tgtctcagaa agttgacatg ctggcagtga gttaaataag cctctgctac gtaagggtta
    22021 tagcttttgc cttaaaggtg tatttaaaat acatcttatc ttattaagaa tgaggtatca
    22081 gctatggctt atcgcgacca acctttaggt gaactggcgc tctctatccc tcgcgcttcg
    22141 gctctgtttc gtaaatatga tatggattac tgctgtggcg gtaagcagac gctggcgcgc
    22201 gcggcggcac gtaaagaact ggatgttgag gtcattgaag ctgaactggc aaagcttgct
    22261 gaacaaccga ttgagaaaga ctggcgtagc gccccgctgg cagaaatcat tgaccatatc
    22321 atcgtgcgct accacgatcg tcaccgcgag cagctgccgg agctgatcct gcaagcgact
    22381 aaagtcgagc gcgttcacgc cgacaaaccg agcgtgccaa aagggctgac aaaatacctg
    22441 accatgctgc atgaagagct ttccagccac atgatgaaag aagagcagat cctcttcccg
    22501 atgatcaaac aaggcatggg cagccaggca atggggccaa tcagcgtaat ggaaagcgag
    22561 cacgatgaag cgggcgaact gctggaagtg atcaaacaca ctaccaataa cgtcacaccg
    22621 ccgccagaag cctgcaccac ctggaaagcg atgtataacg gcattaatga actgattgat
    22681 gacctgatgg atcacatcag tctggaaaac aatgtactgt tcccacgcgc cctagcgggt
    22741 gagtgatcta aaagctggat ggcattgcgc catccagcat gataatgcgg ttatttccgc
    22801 agttcagcag cccgcttctt accaataaac agccagccca accccagcgc gataaaccac
    22861 agcggggtaa ccagcagcgc ctggcgagtg tcatcttcca gtgtcagcaa cacgaccacg
    22921 aacacaaaga acgccataca tacccagcac atcagcttgc cgagcggcat cttgtagatc
    22981 gacttttcat gcagatgagg acgctgtttg cggtacacca ggtacgagca aaggataatc
    23041 gtccagacga acataaacag aatcgcggaa acggttgtaa tcatcgtgaa cgcgccaatc
    23101 acgctaggat tcacatacaa catcaccacg ccgccgagca gacagataca cgagaaggtc
    23161 aaccctttcg cgggtactgc gcgcttagaa agtttagcga acgctttcgg cgcaacacct
    23221 tcctgcgcca gaccaaacag catacggctg gtagagaaga caccgctgtt agcggaagac
    23281 gccgcagagg tcagcaccac aaagttgatc acgctggcag ccgcaggcaa acctaccaac
    23341 acaaacagct caacgaacgg gcttttctcc gggactaccg aactccacgg tgtcacggac
    23401 ataatcacaa tcagcgcgaa gacgtagaac ataatgatac ggatcggaat ggagttaatc
    23461 gcgcgtggca gtgatttctc cggatctttg gtttccgcag ctgttgtacc taccagctca
    23521 atccccacga aagcgaaaac cgctatctgg aatccggcaa agaagccact taaacctttc
    23581 gggaaccagc cgccgtcatt ccacaaatgc gcgaatgacg cttccacacc ggtcggtgac
    23641 tgaaagtgca tcgccaccat gaccaggccg acgacaatca gcgacacgat ggcgacgatt
    23701 ttgatcatcg caaaccagaa ctccatctca ccgaacattt tcacggtggc aaggttgagc
    23761 gtcagcagca gcactatcac cgccagcgag gcgacccagt cggagagatc ggggaaccag
    23821 aactgagcat aagccgtgat cgccaccacg tctgccatac cggttacaac ccagcagaac
    23881 cagtaagtcc agccggtgaa atatcccgcc cacggcccca gtaaatcgga agcgaagtca
    23941 ctaaaagatt tgtattccag attcgaaagc agcaattccc ccattgcccg catcacgaaa
    24001 aagagcataa aaccaatgat catataaacg aaaatgatcg acggcccggc aaggctaatc
    24061 gttttgccgg accccataaa caacccagta ccaatggcac ttccaatggc aataagctga
    24121 acatgtcggt ttgtgagatt gcgccgtagc gactgttcag ccggagcctg atcatcggca
    24181 acgactttta cctgatctac catgtttttt cttcctgtac ctgtctgtgt tgttcaggct
    24241 ctacggcctt tagtcagtct atgatacgac gaatctgtat cgggactcat cgatattagg
    24301 taacaatacg cgggatgaat actaagattt agatctaatg ttaattttat gtttaaagtg
    24361 agtgtcatat cacggtgata acgcgaaaca gctcacaaaa atacacgctt acggggtctt
    24421 tgcaacacaa aatagcgccg tttggcaaac tgaagggttt attgctgaat gcctgctccc
    24481 ctctggttcg aggggaacag aatgcgctgc ttagaggatt tccagcagtt cgacttcaaa
    24541 caccagggtg ctgaacggag ggatggatgc acctgcgccg cgctcgccat atgccagttc
    24601 ctgcgggata gtcagttccc atttagaacc taccggcatc agagtcagtg cttcaatcca
    24661 gccagggatc acgccattaa ccgggaattc agcgggttca ccacgagcaa cggagctgtc
    24721 aaacacggtg ccgtcgatca gtttaccggt gtaatgaaca cgaacgcggt cggtacgtgc
    24781 cggaattgcg ccttcaccct ggttgatcac gcggaattgc aggccagatt cggtgctatt
    24841 cacaccttct tttttggcgt tttcttccag gtatttcacg ccttcagcag ccatcgcctg
    24901 gaaacgctga cgacgaacgg catcggcgcg ttcgtggatt tcacgcagcg cgcgatgcac
    24961 cacatcaacc ggcacagccg gatgtttgcc ttccagcgca tcggcaatac ctgcaaccag
    25021 tgcttctggc agcagccctt ccaggccaga ttcactcagt tgttgcccta cctgcaaacc
    25081 aatgccgtag cttgcttgcg cttcgatggt gtcaaaagtt ggggtggtca tcgtatttcc
    25141 tttcttaggg tataaaagta gcgggcagca tatcagccac acttctttgg gtaaaacttt
    25201 gtcgcaggaa tgatgacaaa tcccggggaa aaagaaacaa tagcaatatc ccgtatgaat
    25261 ataataaagg cgggtttacc acggacatca tagcgttagc ttattcatag gctatgattg
    25321 aggaacaaga cgtggagcag gaggaaacca tgcccgggcg ctttgaacta aaaccaaccc
    25381 tggagaaagt ctggcacgcg ccggacaatt ttcgcttcat ggacccgctg ccgccgatgc
    25441 atcgtcgggg gatcattatt gccgccattg tattggtggt cggatttctg cttccatctg
    25501 atgatacgcc caacgcgtcg gtggtgacgc gtgaagcgca gttagatatt cagtcgcaat
    25561 cacaaccgcc gacagaagaa cagccgcaaa ctacccaaac gcaaccattc cagccagata
    25621 gcggaattga taatcaatgg cgctcttacc gtgtcgaacc cggtaaaacg atggcgcagc
    25681 tgttccgcga ccacggactg cccgctaccg atgtctatgc aatggcgcag gtagaaggcg
    25741 cgggtaagcc gctgagtaat ttgcaaaatg ggcagatggt gaaaatccgc cagaacgcca
    25801 gcggcgtggt gacgggctta accattgata cggggaataa tcaacaggtc ctgtttaccc
    25861 gccagccgga tggcagtttt attcgtgcgc ggtagatgaa gggtcaacca aaagccagct
    25921 aaggattgtc tctttgatga ccttacgtct ggcttccagt gcctgggggg aaaccatact
    25981 gatatgataa actaggccaa gcgtatgctg gttgatcaaa taatatcccc caagcgcagc
    26041 aatattaata ttcacctgca atgggtcgat acctggctta aaaatattgt atttcttccc
    26101 ttcatccaat aaggattcca ttaactgcaa gtgagcatgg ttaatttcta agagacgctg
    26161 tgattttgca taatgcacac ctttactttg attttcacta tgcacaattt ttaaaaacca
    26221 aggattagca atataataat cccatgtaaa atcaataatt ttctcaatgg ctttttgtgg
    26281 cggaagatct gagaaatcaa gggccttttc tttttgtcga atatcgttcc aggtatattc
    26341 cagcacttcg ataaataaat tttctttatt accaaagtgg tgataaaccg tctgtttact
    26401 acaccctgct gcctttacaa tattatctac tctggcccct tcataaccat attcggcaaa
    26461 ttcattaacc gcacatagca gcaatttttc cttcaggctc tgtacgttgc tggtatcaat
    26521 atacataacg cttccccacg acctcttcgt ggtgactatc ataacctgat ttaatttcag
    26581 cttcagaaaa aagggcaaaa acttgtactt tcatcaggtt atcaaatgtc ctgtgatcgc
    26641 catcgcatat caaccaacta gatggttgat ctttttatcc tccagtcgca agctaatcat
    26701 caccaaggaa acaaaattca aaaaagagag ctattgtatg aaatccactc gtcctgttgc
    26761 cgtgattacc ggagccactc gcggtattgg aaagggctgt gcgcaggagc ttgctcgtgg
    26821 cgggtttaac ctgctgatta acgatcgtcc cgatgccgac agcgtagaaa aactgcatat
    26881 cacacaacaa gaatgtttgg cggaaggcgt ggaagtgatt tgcttccctg ctgatgtcgg
    26941 tgatctatct ctgcatgagg agatgctcga cgcggcgcaa aaccagtggg ggcggctgga
    27001 ctgtctgctc aataacgcgg ggatttcagt gaaaaaacgc ggtgacctcc tcgacctcga
    27061 accagacagc ttcgatcaaa acattgccat taacacccgc gcgccgttct tccttgctca
    27121 ggcattcagt aagcgcttac tggcacagcc gaaaccggaa gcggagctgc cgcaccgctc
    27181 gattattttc gtcagttcca tcaacgccat catgctggcg atgaatcgcg gcgaatacac
    27241 cattgccaaa accgccgttt ctgccgctgc tcgtctgttt gctgctcgcc tttgtaatga
    27301 acaaattggc gtttatgaag tgcgccctgg tctcatcaaa actgatatga ctattccggc
    27361 aacggcttat tacgacgaac tgattgccaa aggtctggtg ccgtggggac gttggggtta
    27421 tccggctgat attgcttcca ccgtccgcgc gatggcggaa ggtaaactga tttacacctg
    27481 tggtcaggcg gtcgcaatcg acggcggctt aagtatgccg cgcttctgag gctgaatcat
    27541 gagagatctg ttacaacgcc cggatttatt cagtatcaat accgcgacac tgggctataa
    27601 aacgccgcta cccgccatta ttgatgcctg cgccgcacgc ggtattggcg caattgcccc
    27661 ctggcgcagg gagttgcagg gtgaagatct ccagcaaatc acccgccagc ttgccgccag
    27721 taacatgagc gtttccggtt tgtgccgtag cacttactac accgcgccga cactggcgga
    27781 acgcaaactg gcgattgatg ataaccgccg ggcgctggac gacgcagcgg tgcttaacgc
    27841 cgcctgttat atgcaggtgg ttggcggtct gccaacgggc actaaagatc tatatgaagc
    27901 gcgagaacaa gtgaaacaag ggattcgcca gttgctgcca cattcgaaag atgtcggcgt
    27961 gccaattgcg ctggagccac tgcacccgat gacagctgcc gatcgctctt gcctgtgcac
    28021 gttacgtcag gcgctggact ggtgtgatga actcgatccc gatggtgagt ttggcctcgg
    28081 cgtggcggtg gatgtttatc acgtctggtg ggatccggat ctcgccagtc agatcctgcg
    28141 tgccggaaaa cgcatcctcg cgtttcatgt ttccgactgg ttaatgccga ccactgatct
    28201 ggtcaatgac cgtggtatgc cgggagatgg cgtcatcaat attccgtcaa ttcgtcggct
    28261 ggttgaaaac gccggattta atggtgctat tgaactggaa atattctcgc cttactggtg
    28321 gcagaaagat attaacag
//
LOCUS       DAEEUE010000049        27788 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.049, whole genome shotgun sequence.
ACCESSION   DAEEUE010000049 DAEEUE010000000
VERSION     DAEEUE010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27788
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.049"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..101)
                     /locus_tag="KLK84_002239"
     CDS             complement(<1..101)
                     /locus_tag="KLK84_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE2009549.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCS"
     gene            291..734
                     /locus_tag="KLK84_002240"
     CDS             291..734
                     /locus_tag="KLK84_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE2009550.1"
                     /translation="MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            970..1788
                     /gene="ybhA"
                     /locus_tag="KLK84_002241"
     CDS             970..1788
                     /gene="ybhA"
                     /locus_tag="KLK84_002241"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HBE2009551.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1789..2847)
                     /gene="modC"
                     /locus_tag="KLK84_002242"
     CDS             complement(1789..2847)
                     /gene="modC"
                     /locus_tag="KLK84_002242"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HBE2009552.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2850..3539)
                     /gene="modB"
                     /locus_tag="KLK84_002243"
     CDS             complement(2850..3539)
                     /gene="modB"
                     /locus_tag="KLK84_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBE2009553.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3539..4312)
                     /gene="modA"
                     /locus_tag="KLK84_002244"
     CDS             complement(3539..4312)
                     /gene="modA"
                     /locus_tag="KLK84_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBE2009554.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4479..4628)
                     /gene="acrZ"
                     /locus_tag="KLK84_002245"
     CDS             complement(4479..4628)
                     /gene="acrZ"
                     /locus_tag="KLK84_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HBE2009555.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4757..5545
                     /gene="modE"
                     /locus_tag="KLK84_002246"
     CDS             4757..5545
                     /gene="modE"
                     /locus_tag="KLK84_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HBE2009556.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5613..7085
                     /gene="modF"
                     /locus_tag="KLK84_002247"
     CDS             5613..7085
                     /gene="modF"
                     /locus_tag="KLK84_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HBE2009557.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7346..8362
                     /gene="galE"
                     /locus_tag="KLK84_002248"
     CDS             7346..8362
                     /gene="galE"
                     /locus_tag="KLK84_002248"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBE2009558.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8372..9418
                     /gene="galT"
                     /locus_tag="KLK84_002249"
     CDS             8372..9418
                     /gene="galT"
                     /locus_tag="KLK84_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBE2009559.1"
                     /translation="MTQFNLVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9422..10570
                     /gene="galK"
                     /locus_tag="KLK84_002250"
     CDS             9422..10570
                     /gene="galK"
                     /locus_tag="KLK84_002250"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBE2009560.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10564..11604
                     /gene="galM"
                     /locus_tag="KLK84_002251"
     CDS             10564..11604
                     /gene="galM"
                     /locus_tag="KLK84_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HBE2009561.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11807..12559
                     /gene="gpmA"
                     /locus_tag="KLK84_002252"
     CDS             11807..12559
                     /gene="gpmA"
                     /locus_tag="KLK84_002252"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBE2009562.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12726..13778)
                     /gene="aroG"
                     /locus_tag="KLK84_002253"
     CDS             complement(12726..13778)
                     /gene="aroG"
                     /locus_tag="KLK84_002253"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HBE2009563.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            14094..14474
                     /locus_tag="KLK84_002254"
     CDS             14094..14474
                     /locus_tag="KLK84_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HBE2009564.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14588..15529
                     /gene="zitB"
                     /locus_tag="KLK84_002255"
     CDS             14588..15529
                     /gene="zitB"
                     /locus_tag="KLK84_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HBE2009565.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15526..16245)
                     /gene="pnuC"
                     /locus_tag="KLK84_002256"
     CDS             complement(15526..16245)
                     /gene="pnuC"
                     /locus_tag="KLK84_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HBE2009566.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16283..17326)
                     /gene="nadA"
                     /locus_tag="KLK84_002257"
     CDS             complement(16283..17326)
                     /gene="nadA"
                     /locus_tag="KLK84_002257"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBE2009567.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17575..17650)
                     /locus_tag="KLK84_002258"
     tRNA            complement(17575..17650)
                     /locus_tag="KLK84_002258"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17615..17617),aa:Lys,seq:ttt)
     gene            complement(17654..17729)
                     /locus_tag="KLK84_002259"
     tRNA            complement(17654..17729)
                     /locus_tag="KLK84_002259"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17694..17696),aa:Val,seq:tac)
     gene            complement(17781..17856)
                     /locus_tag="KLK84_002260"
     tRNA            complement(17781..17856)
                     /locus_tag="KLK84_002260"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17821..17823),aa:Lys,seq:ttt)
     gene            complement(17859..17934)
                     /locus_tag="KLK84_002261"
     tRNA            complement(17859..17934)
                     /locus_tag="KLK84_002261"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17899..17901),aa:Val,seq:tac)
     gene            complement(18071..18146)
                     /locus_tag="KLK84_002262"
     tRNA            complement(18071..18146)
                     /locus_tag="KLK84_002262"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18111..18113),aa:Lys,seq:ttt)
     gene            complement(18311..19102)
                     /gene="cpoB"
                     /locus_tag="KLK84_002263"
     CDS             complement(18311..19102)
                     /gene="cpoB"
                     /locus_tag="KLK84_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HBE2009568.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(19112..19633)
                     /gene="pal"
                     /locus_tag="KLK84_002264"
     CDS             complement(19112..19633)
                     /gene="pal"
                     /locus_tag="KLK84_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HBE2009569.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19668..20960)
                     /gene="tolB"
                     /locus_tag="KLK84_002265"
     CDS             complement(19668..20960)
                     /gene="tolB"
                     /locus_tag="KLK84_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HBE2009570.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(21093..22334)
                     /gene="tolA"
                     /locus_tag="KLK84_002266"
     CDS             complement(21093..22334)
                     /gene="tolA"
                     /locus_tag="KLK84_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HBE2009571.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22399..22827)
                     /gene="tolR"
                     /locus_tag="KLK84_002267"
     CDS             complement(22399..22827)
                     /gene="tolR"
                     /locus_tag="KLK84_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HBE2009572.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22831..23523)
                     /gene="tolQ"
                     /locus_tag="KLK84_002268"
     CDS             complement(22831..23523)
                     /gene="tolQ"
                     /locus_tag="KLK84_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HBE2009573.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFVALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23520..23924)
                     /gene="ybgC"
                     /locus_tag="KLK84_002269"
     CDS             complement(23520..23924)
                     /gene="ybgC"
                     /locus_tag="KLK84_002269"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBE2009574.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(24074..24367)
                     /gene="ybgE"
                     /locus_tag="KLK84_002270"
     CDS             complement(24074..24367)
                     /gene="ybgE"
                     /locus_tag="KLK84_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HBE2009575.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24367..24480)
                     /gene="cydX"
                     /locus_tag="KLK84_002271"
     CDS             complement(24367..24480)
                     /gene="cydX"
                     /locus_tag="KLK84_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HBE2009576.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24495..25634)
                     /gene="cydB"
                     /locus_tag="KLK84_002272"
     CDS             complement(24495..25634)
                     /gene="cydB"
                     /locus_tag="KLK84_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBE2009577.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25650..27218)
                     /gene="cydA"
                     /locus_tag="KLK84_002273"
     CDS             complement(25650..27218)
                     /gene="cydA"
                     /locus_tag="KLK84_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HBE2009578.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         7026 a         7343 c         6784 g         6635 t
ORIGIN      
        1 tgagagcaga gatagcgctg atgtccggca gtgcttttgc cgttacgcac cacgccttca
       61 gtagcggagc aggaaggaca tctgatggaa atggaagcca cgcaagcacc ttaaaatcac
      121 catcatacac taaatcagta agttggcagc attaccgtct tgccgaagaa gatccagctt
      181 ctgcaaccag gccattcaga actgaagtga agcgcagtcg actgttaatt gacgagtatc
      241 tgttaatcag gaaaatagca gacgtccaga acgaatggtt cggcctttgc atggatcttg
      301 cactggtaac aggacagcgc aaaggagatc tggcggctat gagatgggag gatataagag
      361 atagcaggct atacgtggaa caacaaaaaa caggggcaaa aattcgcatt tctctcccca
      421 ccacgatttc cagattgaat ttaacacttg ccgatgtact ggataacttg aaaaaaataa
      481 acggtaaaaa cgaaaagtta ctcggaggga agacagccag gacaattgca gcacagttca
      541 ggattgcccg agacacatcc ggattgaaat gggaaggtga cccaccgcca tttcatgaaa
      601 tccgaagttt atccggtcgc ctgcactcag cagaaaaagg cagtgatttc actcaggcgc
      661 tccttggaca tcgttcatcc agcatgacgg ataaataccg tgacggacga gggagggaat
      721 ggaaagacat ttagaattaa tttcgaatga tttcgaaatg ccacttgtaa ttaattgatt
      781 ataaagaatt aattcctaaa gagcatttgc ttcatgaatg ctcctttgca tttagcaact
      841 gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg ctgaattaaa acgcagaatt
      901 ttgtccgata gtggtatcgg tgtaccattc gcgcagacga aaattcttaa ccttaatctg
      961 gaatacgcca tgaccacacg cgtgattgct ctcgacttag acggcacctt attgaccccg
     1021 aaaaagacct tgcttccatc atcgatagaa gccctggccc gcgctcgcga agcaggctat
     1081 caattaatca tcgtcacagg tcgccatcac gtcgctattc atccttttta tcaggcgctg
     1141 gcgctggata cacctgctat ttgctgtaat ggcacctatt tgtatgatta tcatgcaaaa
     1201 accgtgctgg aagcggaccc aatgcccgtt aataaagccc tgcaactcat tgagatgctg
     1261 aatgaacacc acattcacgg tctgatgtat gtcgatgatg caatggtcta tgagcacccg
     1321 accgggcatg tcattcgcac gtctaactgg gcgcaaaccc tgccgccgga acagcgtccg
     1381 actttcacac aagtcgcttc tctggctgaa acgacgcaac aagttaacgc cgtatggaag
     1441 ttcgccctga cgcacgatga cctgccgcaa ttgcagcatt ttggtaagca tgtcgaacat
     1501 gaactgggac tggagtgtga atggtcctgg cacgatcagg ttgatattgc acacggcggc
     1561 aacagcaaag gtaaacgttt gacgaaatgg gttgaggcgc aaggttggtc gatggaaaac
     1621 gtcgtggcat tcggcgataa ctttaatgat atcagtatgc tggaagccgc tggtacaggc
     1681 gtggcgatgg gcaacgccga tgacgcggta aaagcgcgcg ccaacattgt gattggtgat
     1741 aacaccaccg acagcattgc ccagttcatt tatagccacc tgatttaatc aggcggttat
     1801 cgacacactt ttaatttgcg cgtacagcca caggccaggt ttgatcgcca gttcatccct
     1861 ggcccacggg ctgatacgcg cccacagcgt tttaccgccg acttccagct ccacttccac
     1921 ctggccgttg tcgtcataac tattaacaac ttttgcccgc aatacgttac gaatgctggt
     1981 ttgctgcggc ggttgtaaaa ccagagaaac atcggaagcc tgaatgcgga tacgtagcgc
     2041 agcttgcagc ggttcgccca gcttattgac ccacaaatgc tgatcgccca gcgccagcgc
     2101 ggtcatcgcg taatgcggat gatgctcaag caccgtcact ttcagaatgc tactttgttg
     2161 ctctttcggc agccacggat tcatcacgct actgccccac acttcctcca gcgcgccaaa
     2221 ggctttcacc tgaccgtttt ccagtaccat cactctgtct gccagatgga ggatctcatc
     2281 cagcgaatgg ctgacataca acatcggaat gttgatttcc cgtgtcagcc gttgcagata
     2341 aggcaacagt tcgcgtttac gcggaatatc cagtgacgcc agcggttcat ccagcaacag
     2401 caattccggt gctgtcagca aagcccgacc aatcgccacg cgctgttttt cgcctccgga
     2461 caggctgcct ggtaaacggt caagcaacgg ttcaatgcct aaaagcgcca ccagcttatc
     2521 gaactgatcg accatacttt tcgacatgcc gtagcgcaga ttgccacgca ctttgtaatg
     2581 cgggaacaac cgcgcatcct gaaaaacata gccaacacga cgcttttccg gcgtcaggca
     2641 gatacctttt tcggcatcat ttagtacccg cccattgagg acaatccgcc ctttttgcgg
     2701 gcgcgtcagt ccactgatgg cgttaatcag cgaagttttt ccggcaccgg agacgccaaa
     2761 gatagcagtg atgccattgg cgggcagcgt ttcattaatg gtcaggcaat ggttgcccaa
     2821 cgtctgggaa aaattcagct ccagcatgat tagcgccccg cccgttcacg gctgattctg
     2881 gccagccatt ctgaaatcaa cagggagatc atcgccagcg caatagaaat aatgcacagt
     2941 ctcgccgcgc cactttcccc gccgggggtc tggatcaggg tatacatggc agaaggaatg
     3001 gttcgcgttt cgctaggaat gttcgacaca aaggtgatgg ttgcaccaaa ctcaccgaga
     3061 gaacgagcaa aagccagtac cgtaccaaca ataattcccg gtaaggtcag cggtaacgtg
     3121 atagtaaaga aaacgcgcca gcgcccggca cctagtgttc ttgcggcctg ttccagtttg
     3181 acatcaaccc cttccagcgc cagacgaatt gcccgcacca tcagcggaaa cgacatgacg
     3241 gcggcagcga gaaccgcgcc gcgccagcta aaggcgaagg taataccaaa ccagtcatac
     3301 agacgttcac cgataaatcc gcgccgtccc atcgaaacta ataataagta accgacgacc
     3361 acgggcggta acaccagcgg tagatgcagt acgctgtcga gcagagcttt gcccggaaac
     3421 gtgcaacgca ccagtaacca ggcaaaaaag atcccaaacg gcaggctaaa cagcacagcc
     3481 agggaagaaa ctttcaggct taataaaact gcctgccatt ctggatcggt cagtatcatt
     3541 acttggttgt aaatccgtaa cgtttaaaga tttccgctgc ctgcggtccc ttcagataat
     3601 cataaaatgc tttcactgtc gcattgttat gcccttccac aacagcaacc ggatattcca
     3661 cttttttatg tgaatcttcc gggaaggtgg caaccacttt tacccctttg ctggcaactg
     3721 cgtcagaacc gtagacaatg cccagaggcg cttcgttacg ttcgaccagc gccagcgccc
     3781 cacgaacatc ttccgctggg gccagtttcg gagagagcgt atcccatgcg cccagttttt
     3841 gcagtgcttc ttttgcataa atgccagcgg gaacatgttc cggatcgcca accgccaggc
     3901 gaccgccatt cagcagcgaa gtccagttag ttttgctgtc gatggtgaaa tctttctgct
     3961 cgctggcttt cggtgctaca acgaccaggc tattgccgag cagtgtctga cgcgtagcag
     4021 tatcgatcgc ttttttatca accgcataat ccatccattt ctgatcggca gaaataaaca
     4081 gatccgcagg ggcacccgct tcaatctgac gggcgagagt agatgacgaa gcgaaagaag
     4141 aaaccacatc cacgcctttc tcttttttat actgcgtagc aatgtcctgc attgcgttag
     4201 tcagtgatgc ggcggcgaac accgtgattt tcccttcatc tgccagtgca ttgccagcaa
     4261 ctgcgaaaga gagtgccgcc ccggcaaaca agttcaacca tttacgagcc attggtaacc
     4321 ccttaatgta acgttatgta ggcgacaata taacgactaa ctcggaattt tcccagcagt
     4381 tattgctaac ctttgtgagg tagataagaa aaaatatcgg caggaaaagc aggaagttga
     4441 gaaaaagaaa atgcccgact aagcgggcac tcagggaatc aatgattttg tccgggctgg
     4501 tcttttttac caacaccaga aaagatgttg aatacttcac caagaccgta aatcagaccc
     4561 aggatgatgg ccatcacgac aggtaccatg attacggcga ataccagact ttttaataac
     4621 tctaacatgg tcaactccag atatagtcat gagactattc taaccgctaa gcacagaaaa
     4681 gcactcccct tttgtgcggt cagctttgcg tggcgttcgt tttccgtcac aataagactt
     4741 ttgccaggac attgttatgc aggccgaaat ccttctcacc cttaagctcc aacaaaaatt
     4801 attcgccgac ccgcgccgca tttcgctact aaaacacatt gcgctttccg gttccattag
     4861 ccagggagcg aaagatgccg gtatcagcta taaaagcgcc tgggatgcca ttaacgagat
     4921 gaatcagtta agtgagcata ttctggtcga gcgcgcaaca ggcggtaaag gtggcggagg
     4981 cgcagtactg acccgctatg gtcagcgact gattcagctc tatgacttac tggcgcaaat
     5041 ccagcaaaaa gcctttgatg tgttaagtga cgatgacgcc ctgccgctga acagcctgct
     5101 ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt aaccagtggt tcggtaccat
     5161 caccgcccgc gatcatgatg acgttcaaca gcatgttgat gtcttactgg ctgacggaaa
     5221 aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgca cgtctggggc tggatgaagg
     5281 caaagaagtg ttgatattgc taaaagcgcc gtgggtaggc attactcagg acgaagcggt
     5341 cgcacaaaac gccgacaacc aattaccggg tattattagt catattgagc gcggcgcaga
     5401 gcagtgcgaa gtattaatgg cgctacccga cgggcaaaca ctgtgcgcca cagtgccggt
     5461 aaatgaagcg acttctcttc agcaagggca gaatgtcacg gcctacttta atgccgacag
     5521 cgtgattatc gccacgctgt gctaagcgtg ttgacaattt gttatgaaac acgtatccct
     5581 gtcagtaatc gctgcacaaa atgggatata aaatgtcatc gttgcaaatt ttgcaaggca
     5641 cgtttcgtct tagcgacaca aaaacgctgc aattgcctca gctaacgtta aacgcgggtg
     5701 atagttgggc gtttgtcggt tcgaatggaa gtggaaagtc ggcgctggcc cgcgcgctgg
     5761 cgggggaact tccgcttttg aaaggtgaac ggcaaagcca gttttcccac atcactcgtc
     5821 tctccttcga gcaattgcaa aagctcgtca gcgacgaatg gcaacggaat aacaccgata
     5881 tgctcggccc tagcgaagat tacaccggac gcactacggc tgagattatt caggatgaag
     5941 taaaggatgc accgcgttgc atgcaactgg cgcagcagtt cggtattacc gccctcctcg
     6001 accgacgctt taaatacctt tccactggcg agacgcgaaa aaccctgctg tgtcaggcgc
     6061 tgatgtcgga gcctgacttg ttgattcttg atgagccgtt cgatggcctg gatgttgcct
     6121 cacgtcagca gctggctgag cgactcgcct cgttacatca gtccggtatt actttggtac
     6181 tggtgctcaa tcgcttcgat aagatcccgg aatttgtcca gtttgctggc gtgctggcgg
     6241 attgtacgtt agcggaaact ggcgctaaag aggaactgct ccagcaagca ctcgtcgcgc
     6301 aacttgcgca tagtgaacag cttgaaggtg tgcaactgcc ggagccggat gaaccttcag
     6361 cacgtcacgc cttacccgcc aacgaatcgc gcattgtgct gaacaatggc gtggtttctt
     6421 ataatgatcg ccccattctt aataacctta gctggcaggt gaatccaggc gaacactggc
     6481 aaattgtcgg gccaaatggc gcgggaaaat cgacgttatt aagcctgatt actggcgatc
     6541 atccgcaagg ttacagcaac gatttgacgc ttttcggccg acgtcgcggc agcagcgaaa
     6601 ccatctggga catcaaaaag catatcggtt acgtcagcag tagtttgcat ctggattacc
     6661 gggtcagcac taccgtgcgt aatgtgattc tttctggcta ttttgattcg attggcattt
     6721 atcaggtcgt ttcggatcgc cagcaaaaac tggtgcagca gtggctggat attctcggca
     6781 ttgataaaca cacggctgac gctccgttcc atagtctttc ctggggacag cagcgtctgg
     6841 cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct tattctcgat gaaccactac
     6901 aggggcttga tccgctcaat cgccaactta tccgccgttt tgttgatgtg ctgattagcg
     6961 aaggtgaaac gcaattgttg tttgtttcgc accacgctga agatgcgcct gcctgtatta
     7021 cccatcgcct tgagttcgtg ccggacggtg gactctatcg ctatgtgctg acaaaaatat
     7081 attgagtcgg tagtgctgac cttgccggag gcggcctaag cgcactctcc ggccaacggt
     7141 tcgacgcatg caggcatgaa accgcttctt ttttcagata aaaagcgcaa tcattcataa
     7201 accctctgtt ttataatcac ttaatcgcgc ataaaaaacg gctaaattct tgtgtaaacg
     7261 attccactaa tttattccat gtcacacttt tcgcatcttt gttatgctat ggttatttca
     7321 taccacaagc ctaatggagc gaattatgag agttctggtt accggtggta gcggttacat
     7381 tggaagtcat acctgcgtgc aattactgca aaacggtcat gatgtcatca ttcttgataa
     7441 cctctgtaac agtaagcgca gcgtactgcc tgttatcgag cgtttaggcg gcaaacatcc
     7501 aacgtttgtt gaaggcgata ttcgtaacga agcgttgatg accgagatcc tgcacgatca
     7561 cgctatcgac accgtgatcc acttcgccgg gctgaaagcc gtcgccgaat cggtacaaaa
     7621 accgctggaa tattacgaca acaatgtcaa cggcactctg cgcctgatta gcgccatgcg
     7681 cgccgctaac gtcaaaaact ttatttttag ctcctccgcc accgtttatg gcgatcagcc
     7741 ccaaattcca tacgttgaaa gcttcccgac cggcacaccg caaagccctt atggcaaaag
     7801 caaattgatg gtggaacaga tcctcaccga cctgcaaaaa gcccagccgg actggagcat
     7861 tgccctgctg cgctacttca acccggttgg cgcgcatccg tcgggcgata tgggcgaaga
     7921 tccgcaaggc attccgaata acctgatgcc atacatcgcc caagttgctg taggccgtcg
     7981 cgactcgctg gcgatttttg gtaacgatta tccgaccgaa gatggtactg gcgtacgcga
     8041 ttacatccac gtaatggatc tggcggacgg tcacgtcgtg gcgatggaaa aactggcgaa
     8101 caagccaggc gtacacatct acaacctcgg tgctggcgta ggcagcagcg tgctggacgt
     8161 ggttaatgcc ttcagcaaag cctgcggcaa accggttaac tatcattttg caccgcgtcg
     8221 cgaaggcgac cttccggcct actgggcgga cgccagcaaa gccgaccgtg aactgaactg
     8281 gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc tggcactggc agtcacgcca
     8341 tccacaggga tatcccgatt aaggaacgac catgacgcaa tttaatctcg ttgatcatcc
     8401 acatcgccgc tacaacccgc tcaccgggca atggattctg gtttcaccgc accgcgctaa
     8461 gcgcccctgg cagggggcgc aggaaacgcc agccaaacag gtgttacctg cgcacgatcc
     8521 agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc gataaaaacc ccgattacac
     8581 cgggacttac gttttcacta atgactttgc ggccttgatg tctgacacgc cagatgcgcc
     8641 agaaagcaac gatcctttga tgcgttgcca gagcgcgcgc ggtaccagcc gggtgatctg
     8701 cttttcaccg gatcacagta aaacgttgcc agaactgagc gttgcggcat tgacggaaat
     8761 cgtcaaaacc tggcaggagc aaaccgcaga gctgggaaaa acgtacccgt gggtgcaggt
     8821 ctttgaaaac aaaggtgcgg cgatgggctg ctctaacccg catccgcacg gacagatttg
     8881 ggcaaatagc ttcctgccta acgaagctga gcgcgaagac cgcctgcaaa aagaatattt
     8941 tgccgaacag aaatcaccaa tgctggtgga ttatgttcag cgcgagctgg cagacggtag
     9001 ccgtaccgtt gtcgaaaccg aacactggtt agccgttgtg ccttactggg ctgcctggcc
     9061 gttcgaaacg ctactgctgc ccaaagccca cgttttgcgg atcaccgatt tgaccgacgc
     9121 ccagcgcagc gatttggcgc tggcgttgaa aaagctgacc agtcgttatg acaacctctt
     9181 ccagtgctcc ttcccctact ctatgggctg gcacggcgca ccatttaatg gcgaagagaa
     9241 tcaacactgg cagctgcacg cgcactttta tccgcctctg ctgcgctccg ccaccgtacg
     9301 taaatttatg gttggttatg aaatgctggc agagacccag cgagacctga ccgcagaaca
     9361 ggcagcagag cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg gagtgtaaga
     9421 aatgagtctg aaagaaaaaa cacaatctct gtttgccaac gcatttggct accctgccac
     9481 tcacaccatt caggcgcctg gccgcgtgaa tttgattggt gaacacaccg actacaacga
     9541 cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg atcagctgtg caccacgcga
     9601 tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat cagctcgacg agttttccct
     9661 caatgcgccc attgtcgcgc atgaaaacta tcaatgggcg aactacgttc gtggcgtggt
     9721 gaaacatctg caactgcgta acaacagctt cggcggtgtg gacatggtga tcagcggcaa
     9781 tgtgccgcag ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg tcggaaccgt
     9841 attgcagcag ctttatcatc tgccgctgga cggcgcacaa atcgcgctta acggtcagga
     9901 agcagaaaac cagtttgttg gctgtaactg cgggatcatg gatcagctaa tttccgcact
     9961 cggcaagaaa gatcatgcct tgctgattga ctgccgctca ctggggacta aagcagtttc
    10021 catgccgaaa ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta ccctggttgg
    10081 cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct tccagcagcc
    10141 agctctgcgc gatgtcacca ttgaagagtt caacgctgtt gcacatgagc tggacccaat
    10201 cgtggcgaaa cgcgtgcgtc atatcctgac tgaaaacgcc cgcaccgttg aagctgccag
    10261 cgcgctggag caaggcgacc tgaaacgtat gggcgagttg atggccgagt ctcatgcctc
    10321 tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac actctggtag aaatcgtcaa
    10381 agctgtgatt ggcgacaaag gtggcgtacg catgaccggc ggcggatttg gcggctgtat
    10441 cgtcgcgctg atcccggaag agctggtgcc tgccgtacag caagctgtcg ctgaacaata
    10501 tgaagcaaaa acaggtatta aagagacttt ttacgtttgt aaaccatcac aaggagcagg
    10561 acagtgctga acgaaactcc cgcactggca cccgatggtc agccgtaccg actgttaact
    10621 ttgcgtaaca acgcagggat ggtagtcacg ctgatggact ggggtgcgac tttactttcc
    10681 gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc tgctcggctg tgccagtccg
    10741 gaatgctatc aggatcaggc tgcgtttctg ggggcctcta ttggtcgtta tgccaaccgc
    10801 atcgccaata gtcgctatac ctttgacggt gaaaccgtga cgctttcgcc aagccagggc
    10861 gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac gtcgctggca gattgtgaac
    10921 cagaacgatc gtcaggtgct gtttgccctg agttcagatg atggcgatca gggcttcccg
    10981 ggtaatctcg gcgcgacggt gcaatatcgt ctgaccgacg ataaccgtat ctccattact
    11041 tatcgcgcca cagttgataa accttgcccg gtgaatatga ctaatcacgt ctatttcaat
    11101 cttgacggcg agcagtctga cgtgcgcaat cacaagttgc agattctggc ggacgaatat
    11161 cttccggttg atgaaggcgg cattccgcac gacggcttga aatctgtcgc cggaacgtct
    11221 tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc ttgccgacga cgatcagcgc
    11281 aaagtgaaag gttacgatca cgcattcttg ttacaggcca aaggcgatgg caagaaagtg
    11341 gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga aggtctacac caccgctccg
    11401 gctcttcaat tctactccgg caacttcctc ggcggcacac cgtcgcgggg aaccgaacct
    11461 tacgccgact ggcaagggct ggcgttggaa agcgagtttc tgccggacag cccgaaccac
    11521 cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg aagagtattc cagcctgacg
    11581 gaatatcagt ttattgctga gtaatgttaa gccctcgtca tgagggcttt atcttcatat
    11641 tgttcaaatc accagcaaac accgacatat ttgcaactca atattcacaa caaccttaca
    11701 ctgcgccact attttcgcta tggttatgcg taagcattgc tgttgcttcg tcacggcaat
    11761 ataatgagaa ttattatcat taaaagatga tttgaggagt aagcatatgg ctgtaactaa
    11821 gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa gaaaaccgtt tcaccggttg
    11881 gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca aaagcagcag gtaagctgct
    11941 gaaagaggaa ggttacagct ttgactttgc ttacacttct gtgctgaaac gcgctatcca
    12001 taccctgtgg aatgtgctgg acgaactgga tcaggcatgg ctgcccgttg agaaatcctg
    12061 gaaactgaac gaacgtcact acggtgcgtt gcagggtctg aacaaagcgg aaactgctga
    12121 aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt tttgcagtga ctccgccgga
    12181 actgactaaa gatgatgagc gttatccggg tcacgatccg cgttacgcga aactgagcga
    12241 gaaagaattg ccgctgacgg aaagcctggc gctgaccatt gaccgcgtga tcccttactg
    12301 gaatgaaacc attctgccgc gtatgaagag cggtgagcgc gtaatcatcg ccgcacacgg
    12361 taactccctg cgtgcgctgg tgaaatatct tgataacatg agcgaagaag agattcttga
    12421 gctgaatatc ccgactggcg tgccgctggt gtatgagttc gacgagaatt tcaaaccgct
    12481 gaaacgctat tatctgggta atgctgacga gatcgcggcg aaagcagcag ccgttgcgaa
    12541 ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaaaatg agaaagccga ctgcaagtga
    12601 gtcggctttt ttgtttgtcg ttgctaacaa tggagcacat tgcctgatgc gacgctgcgc
    12661 gtcttatcag gcctgtggtg attcatcgga tacgccactc tgacggcgca tccgacaatt
    12721 aaaccttacc cgcgacgcgc ttttactgca ttcaccagtt gacgtaacag agcatcggta
    12781 tcttcccagc caatgcaggc atcggtgatg ctcttaccgt aggccagcgg ctccccgcta
    12841 tcgaggctct gattgccttc caccagatgg ctttccacca tcacgccaat aatggccttt
    12901 tcgccaccgg caatctgctg gcaaacgtca gcacaaacat ccatctgctt tttgaattgt
    12961 ttggacgaat tagcatggct gaaatctatc atcacctgag ctggcagacc tgctttgttc
    13021 agcccttctt tcacttcagc aacgtgcttc gcgctgtagt taggctcttt accgccgcgc
    13081 agaatgatat ggcaatcgcc gttaccactg gtattcacaa tcgccgaatg cccccattta
    13141 gttacggaca ggaagcagtg cggcgcaccg gcggcattaa tggcatcgat agccacttta
    13201 atcgtaccgt ctgtgccatt tttaaaacct accggacaag aaagaccaga cgccagttcg
    13261 cggtgcacct gcgattccgt ggtacgtgca ccaattgcgc cccagctcat caggtcagcg
    13321 agatactgag gagtgatcat atcgagaaac tcacccgccg ctggcagacc gctgtcgtta
    13381 atatcaagca gcaatttacg ggctatacgc agaccgtcgt tgatctggaa gctgttatcc
    13441 atatgcggat cgttaatcaa ccctttccag cccaccgtgg tacgcggctt ttcaaaatag
    13501 acgcgcatta cgatttccag ctcatctttc agctcttcac gcagcgccag caagcgagtg
    13561 gcatactctt tagccgcgac aggatcatga attgagcacg ggccaatcac caccaacagg
    13621 cgatcatcat tacctttcag gatcttatgg atcgcttttc gggcatgggc aaccgtattc
    13681 gcggcatttt cagttgcggg gaatttttcc agcaatgcga caggaggaag taactctttg
    13741 atttctttga tgcgtaaatc gtcgttctga taattcatgt ctgttccagt gttgccatac
    13801 ttatcttagt gaatgcaata cttccaatct atatcttccg tcagaatgtg taaacggggt
    13861 tttacactat gaacggatta atcctggaat tagcaagaaa aacgccagat tgtcgcgaaa
    13921 aacgagatct ctcctacaat ttctaactgt aactcctttg tttgttaatt atttcaagat
    13981 tctctgctgc gtttcataaa ctggctgaaa tcttaaacca atgccttata ttcacctgca
    14041 aatgcactgt tggaagaggt tatccgacat aacgaccata acaggagcat cctatgaaaa
    14101 tgacaaaact ggccacatta tttctgactg ccactctaag ccttgccagc ggtgccgcac
    14161 tggctgccga tagcggagcg caaactaata acggccaggc aaacgccgca gctgatgcgg
    14221 gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa taacgtcgac aataacgggg
    14281 taaataccgg ttctggcggc acaatgctgc attcggatgg ttcttcaatg aacaatgacg
    14341 gaatgaccaa agatgaagag cacaagaaca ccatgtgcaa agatggtcga tgtccggata
    14401 tcaataaaaa ggtacaaacc ggtgatggca tcaataatga tgtcgatacc aaaaccgacg
    14461 gtaccacgca gtaaccctgc aaaatagcca ataatgggag agcggagtct ctccctttta
    14521 atttttcact tctaaaagca gtatgatgtc ataaagttaa gtgagaataa ttaagggatg
    14581 actcgtcatg gcgcactcac actcacacac gtcctcacac ctgccagaag ataataatgc
    14641 tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt atgctggtag aagtcgttgg
    14701 tggttttctt tctggttctc tggcattgct ggccgatgcg ggtcatatgt tgaccgatac
    14761 tgccgccctg cttttcgccc tactcgccgt gcaattttca cgtcgtcctc ccaccattcg
    14821 ccacactttc ggttggctaa gactgaccac gctcgcggcg tttgtgaatg ccatcgccct
    14881 cgtggtaatt accattttga ttgtctggga ggcgatagaa cgcttccgca cgccgcgtcc
    14941 ggtcgaggga ggcatgatga tggcaattgc cgtggctggg ttgctggcaa atatactttc
    15001 tttctggtta cttcatcacg gcagcgaaga gaaaaacctc aacgtacgag cagcggcact
    15061 gcatgtgctc ggggacctgc tgggttcggt tggggctatt attgccgcgt tgattattat
    15121 ctggactggc tggacacctg ctgaccccat tctctcgata ctggtgtcgc ttctggttct
    15181 gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa ttacttgaag gtgcaccggt
    15241 atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg gaaatcccgg aagtccgcaa
    15301 tgtgcaccat gtacatgtat ggatggtagg cgagaagccg gtgatgacgt tgcatgtgca
    15361 ggtgatcccg ccacacgatc acgatgcctt gttggatcag atccaacact acctgatgga
    15421 tcactatcag attgagcaca ccaccattca gatggaatat caaccttgtc ataggccaga
    15481 ctgccacctg aatgaagggg tgtctggcca ttcacatcat caccattaat gggacagcgc
    15541 gcgtgagcct ctttcacgtg cgctgttgat ccacatccgg ctgccgttga gcgcaataaa
    15601 ggtcaggatg atgtactcca gagacatggc gtaaacgccc tgaagtgcaa aaataacgac
    15661 gctaatcacg ttaataatca cccacaacag ccagttttcc acatacttac gcgtcatcag
    15721 aatcattgcc acgatagata acaccatcat gcatgaatcc cagaacggga aagcgtccgg
    15781 ttgcagttca ggcatcacaa cctgtaatcc taatgcttgc atgatcatga ctgccacgcg
    15841 ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc agaccaatcg aaacaacgca
    15901 aaccgccaac cagctgagtg ccttcggcaa tggcaaccag cgaattttca actccgcctc
    15961 gttctgactg gtttgtcgcg accacgcata ccagccgtaa atattcgcgg caaagaaaaa
    16021 cacctgtaat agcaggctgg catacagctg aatctgaaag aaaataatgc caaacaaggt
    16081 aacgttgatc aggccaaaga agtagttgct gatcttctcc agactggcaa ggccaataca
    16141 cagcaacccg gcgatcgtgc ctaccgcttc gatccatgag agatcataac cgcccgcccc
    16201 tattggtata tgtaccagga tattctgcac actaaaaaaa tccatctttc ccccaaagcg
    16261 taacgttacg ccttattatt cgttatccac gtagtgtagc cgcaaaatcc agcatacgat
    16321 tgagcggcac cagcgccctc tctcgcagcc tctcatcgac atgaacctcg tgattacttc
    16381 cttcctgttc taatgcctct gcgatagcct gaaggtcatt catggccatc cacggacaat
    16441 gcgcgcagct gcggcaggtt gcgccctcac ccgcggttgg tgcttccagt aactctttat
    16501 ctggcaccgc ctgctgcatt ttgtagaaaa taccccgatc ggttgccaca ataagcctct
    16561 gatgaggcaa tgcttttgca gcagcgatca gttgactggt ggaaccgacc gcatccgcca
    16621 tatcgacaat agcttgtggt gattctggat gcaccagtat ggcagcatcc gggtattctt
    16681 cttgcaagcg ggttaacgcc tgagtcttaa attcatcatg cacaatgcag gcaccctgcc
    16741 agcataaaat gtctccaccc gtctgttttt gcacgtaacg ccccagatgt ttgtcgggtg
    16801 cccagatgat tttttcaccc aaactatcaa gatgatcaat aagttcgacg gcaatgcttg
    16861 aagttaccac ccaatctgcg cgcgctttta ccgcagcaga agtgttggcg tagacgacga
    16921 cagtacgatc gggatgggca tcgtaaaatg cgttaaattc ttcaacaggg cagccgagat
    16981 ccagtgaaca ctcaacctga agtgtcggca tcagaattgt tttttccgga ctgagaattt
    17041 tggcggtttc tcccataaat ctcaccccag cgactaacaa agtagaagcg ggatgctttg
    17101 caccgaagcg cgccatttcc agagaatcag aaatacagcc accggtttct tctgccagtt
    17161 gttgaatttc gggatcggta tagtagtggg caaccatcac cgcattacgt tcttttagca
    17221 aacgttttat cttctcgcgg taatacgctt tttcatcaat gcttaacggc gtcggcttcg
    17281 gggggaaagg ataaatcgcc gtgtctggat caaacattac gctcatctta ccatctcgtt
    17341 ttacaggctt aacgttaaaa ccgacattag cgtaacattc gctgttatcg gtgtaatttg
    17401 ttttatatgc taaacaagat agtcgaatgg tggggaaaag tcactcgaat tacgcctaat
    17461 tccaccgacg tagagttgct tgcacagtaa gctctgacga tgtcatcagg tttacaaagt
    17521 gaaattgagg atgcctgaat agcaaaaaag cgcctttagg gcgctttttt acattggtgg
    17581 gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt caactgctct accaactgag
    17641 ctaacgaccc gagtggtggg tgatgacggg atcgaaccgc cgaccccctc cttgtaaggg
    17701 aggtgctctc ccagctgagc taatcacccg atactgcgct ggatactaca aactacttcc
    17761 accgaaccca ccgtaattag tggtgggtcg tgcaggattc gaacctgcga ccaattgatt
    17821 aaaagtcaac tgctctacca actgagctaa cgacccggtg gtgggtgatg acgggatcga
    17881 accgccgacc ccctccttgt aagggaggtg ctctcccagc tgagctaatc acccgacact
    17941 acgctggata ctacaactaa ctatgttagt ggtgggtcgt gcaggatgac tcgacttcgt
    18001 ctcgcccttc ggcgccgttg ctgatgcaac gttatccttc acgttttact accgcttcca
    18061 ccttagcgat tggtgggtcg tgcaggattc gaacctgcga ccaattgatt aaaagtcaac
    18121 tgctctacca actgagctaa cgacccactt tttcgttgct ttcggtttgt ttgatatccc
    18181 gtggcaacgg cggcatatat tactgatttc agacttgagc gcaacaaaaa tttcgatgca
    18241 gatcactcaa ctgcttatga ttcgcacgac acgaccagaa ataatgcgac ttctggtcgt
    18301 gtgttatgca ttacatcgcg ttcagacgtt tttgtgcctg tttagcgcca tcagtaccag
    18361 ggtatttact gataacctgc tggtatacgg ctttcgcttt tgcggtgtca cctttgtcct
    18421 gcatgatgac gccgacttta aacatcgcat cagcagcctt tggtgacttc ggatagtttt
    18481 tcactaccga agcaaaatag tacgccgcat catctttttt acccttgttg tagtttaact
    18541 gacccagcca ataattggcg tttggcaggt aagttgaatc agggtaattt ttgatgaaat
    18601 tctgaaatgc caccattgcg tcatcctggc gggatttatc ctgcaccagc gcaatagctg
    18661 cattgtaatc cgtgtttgca tcgccgcttt tcaccggcgc gccagcattc gcagtaccag
    18721 catcagctgt cggcgtcgtt gatgccgtcg caccgctttg atcgctgctg gttgattgcg
    18781 ccgctgcacc accgctgctg aggctgtcga tctgcaacag gatctgcttt tgccgctcca
    18841 cgacctgatt cagttgatac tgattttcct gaatctgacc acgcagggaa tcaatatcgg
    18901 attgattatc agaaagttgt tgctggagtt gggttaaaag ctggctgtga gcattagaaa
    18961 tacgctcaag ttgagtgacg cggtcttcga ccgagcctga gccgacacta ctgattggtg
    19021 cctgagcaaa agcggcccag ggggccgcta taccaaccag taacgacaga ctcaatagtt
    19081 gatgtctgaa gttactgctc atgcaattct cttagtaaac cagtaccgca cgacggtttt
    19141 tggagtatgc cgcttcgtca tgacccagta ctgcaggttt ttctttaccg taagaaacga
    19201 tggagatctg gtctgcagaa acgcctttac cctgcaggta catcttaacg gcgttcgcac
    19261 gacgttcacc cagggagatg ttgtattccg gagtaccacg ttcgtccgcg tgaccttcta
    19321 cggtgacttt gtaagacggg ttgctacgca ggaagtttgc atgtgcatcc agcatttgag
    19381 cgaagtcaga acggatatcg tacttgtcca gatcgaagta aacgatgttg ttctgctgca
    19441 gctgttgcat ttgcagacga gcctgctctt cggaagacat gttgccgttg ccgccgttcg
    19501 catccatacc agtgccggca cccagcatgc cttcgctgcc gtcattgctg gcgttcttgt
    19561 tggaagaaca tgccgcaatt gccataacag gcagagcaat catcagccct ttcagcactt
    19621 tgttcagttg catttcaatg attcctttac tattcaatta attattatca cagatacggc
    19681 gaccaggcag ggaatttgac ctgtccatca gttgccggaa gacgcgcttt gaaacgccca
    19741 tctgtagaaa ccaaattcag cacggatccc atcccctgag aagagctgta gattaccata
    19801 gtgccgttag gtgccagact tggcgtttca tccaggaacg tggacgacag aacttgtacg
    19861 cctcccgttg ccagatcttg tttggcaatg tgctgctgcc caccattgga gctgaccatt
    19921 accataaatt taccgtcgct gctgacatcc gcatcctggt tctgcgaacc ttcccaggta
    19981 atacgttgtg gcgcaccgcc gttgatattc actttataaa cctgcggacg accggcctgg
    20041 tcagaagtaa atgccaggtt ctggctatcc gggaaccagg tcggttcggt attgttactg
    20101 cgaccatcag tcacctggcg gatctgacca gaagccaaat ccattacgta caggttcaga
    20161 ctaccggttt tcgacaaggc gaatgccagt ttgctgccgt ctggcgagaa tgcaggtgca
    20221 ccgttgtgac gcgggaatga agccacctga cgtacagcgc catttgccag cgtctgaata
    20281 accagcgcgg aacgaccgct ttcgaaggtc acataagcca gtttagaacc gtctggtgac
    20341 cacgccggag acatcagcgg ctgcggtgaa cggtgaacga caaactggtt gtaaccgtca
    20401 tagtcagata cgcgcagttc atacgggaac tgaccgccgt tggtctgaac aacgtaggca
    20461 atacgggtac ggaacgcacc tttaatgccg gtcagttttt caaacacttc atcactggcg
    20521 gtatgaccag cataacgcag ccactgcttg ttcactttgt acgagttctg agcaagtaca
    20581 gtacccggtg cgccgccagt gtcaacaagt tgataagcaa cattgtaaga accatccgga
    20641 ttcggagtga cctgaccgac aactacagcg tcaatgccca gtgcggacca tgcagctggt
    20701 tgtacttcct gcgcactacc cggctgctgt ggcagacgag cgcgatctaa cggattaaat
    20761 ttaccgctgt tacgcaagtc agcagcaacg atgccgccaa tatcttcagg tgccgcacca
    20821 ggccccgccc actggaaagg aacaacacca ataggacgac cggaatctac accgctgtcg
    20881 atcacaatgc ggacttcagc atgcagaact gatgcccaca gtatgagaaa accaaatgct
    20941 actcgtaatg cctgcttcat catatctccc ttatctggac cgatggccca cgataattta
    21001 gcagaatgtt aacaaactaa aatacacaaa actaccagaa ccccgtggca accggtgcct
    21061 gatgttgacc gtccgaacag tcaacatcgc gattacggtt tgaagtccaa tggcgcgttt
    21121 ttgaacactt catatactgc ctggcttggt ggtttcggga tcttcgcaag tttagctgct
    21181 gccaacgcag cctgacaaag tgcgggatcg ccaccttcag gtttgatatc cagtaacata
    21241 ccatcgggtg ccagttttat gcgcagcgta caggttttgc ctgcatagga cgatgcgtca
    21301 tagaacttac tttcgatagc agatttaatc tgcccggcat agttattgat atcggcccct
    21361 gatgcgccat tgtttttagt attaccactc ccggcaggcg aagcattgtt ccctttcgcc
    21421 cctccccccg ttttcggtgc attcttacca gagcttagct caccgaaaat atcatctgcc
    21481 tctgcggcag cttttgctgc agcggctttt tcagctgctg cttttgccgc tgccgctttt
    21541 ttgtctgcgg cggctttttc tgccgctgct ttcttatcag ctgctgcctt atcagcagcc
    21601 gctttttctg ctgctttttt gtcggctgca gctttctctg ctgccgcttt cttatcagct
    21661 gcagcctttt cagcagccgc tttcttctca gcttctgcct tggctttttc agcagcttca
    21721 gttgctgctt tctttcttgc ttcagctgca gctgcttctg ccgcttccgc tttcttcttc
    21781 agtgccgcag cggctacctc ggcttttttc tgcgcttcgg ctgcggcttt ggcggcttct
    21841 gcttctgctt ttttcttcgc gtctgcagcc gctttcttcg ctgcttcttc cgcagcttta
    21901 gcatctgccg ccgctttcgc tgccgccact tcagcttgct tctgctttaa ctcggcctgt
    21961 tttgcggctt cttcagcctg ctttttctgc tcctgagccg ctaaccgctc tttctcaagt
    22021 tgcttcaggc gttcctgttc agccgcttgt ttctcacgca gttcttcagc agcctgctgt
    22081 tccttcatct tgcgctgttc atcagaacgc ttcgcgcttg attcctggct ttgcatgcgt
    22141 ttgtactgct caactaccgc acctgaatca accatgacag cgtcgatgga cgaaccaccg
    22201 ccgcctccgg ctgaagcttc tatattctca tcgaacgaac tccagatcag cgccgcaaat
    22261 aagatgacat gcagcactgc tgaaataatt atcgcccgct tgagcttgtc gttttgttcg
    22321 gttgcctttg acactctcgg tttccaaaaa ctgttcgcct gttacccgct ctctttcaag
    22381 caagggaaac gcagatgttt agataggctg cgtcattaaa ccaaccgatt tcacacccgc
    22441 actatgtaac aagttcagtg ctttaattat ttcatcgtaa ggcacatctt ttgcgccacc
    22501 gatcagaaag accgttttcg ggttggcctt gaaacggctg gacacttccg ccaccacctg
    22561 ctctggtggt aaacgctcca ggcgatcttt ctcaaccacc acggtgtact gaccaatacc
    22621 agacacttca acaatcactg gcggattatc gttactgctc accgcctgtg attcagtagc
    22681 gtctggcaga tcgacctcca cgctctgggt gatgatgggc gctgtcgcca taaagatcag
    22741 caacagcacc agcagtacgt ccagcaacgg aacaatgttg atttcggact tgagatcgcg
    22801 acgacctcgt ccacgcgctc tggccatggc ttaccccttg ttgctctcgc taacggtaaa
    22861 cgcctggcgg tgcagaatcg cggtaaactc ttccataaag ttgtcgtaat tcagttccag
    22921 tttgtttacg cgctggttga gacggttata agccattacc gccggaattg cggcgaacag
    22981 accaattgcc gtcgcaatca acgcttctgc gataccgggc gcaaccattt gcagtgttgc
    23041 ttgttttacc gccccgaggg cgacaaaggc gtgcatgatc ccccagaccg taccaaacag
    23101 accaatatac gggctgatgg agccaaccgt accgaggaac ggaatgtgcg tttccagatt
    23161 ttcaagttca cggttcatgg agatacgcat agcacgcgac gccccttcca ctacggcttc
    23221 cggcgcatgg ctattggcac gatgcaggcg cacaaactct ttgaacccgc tgtaaaagat
    23281 ttgttccgaa ccagtcagat tatcccgttt cccctgactc tcttgataga ggcgagagag
    23341 ttcgattcca gaccagaatt tatcttcaaa cgcttcggct tcacgcgccg ctgcgttaag
    23401 aatacgggtt cgctggataa taatggccca ggatgcgatt gaaaaaccaa tcaaaatcaa
    23461 catgataagt ttaaccagaa ggctagcctt caggaacaaa tcaaggatat tcatgtcagt
    23521 cactgcttaa actccgcgac aatagacttg ggaagcgcac gaggcttcat tttgagtggg
    23581 tcaacgcaaa caaccagaac ctctgcttca ttcagcaaag tattctcggc gttgacaata
    23641 cgttgcgtga aaaccaaaga ggtgccacgc attgatgtta tttcagtctg tatttcgagc
    23701 atatcgtcga gccgcgcagg tgcgtaatat tccaccgtca ttttacgtac cacaaaggca
    23761 acgcgttcag ccatcagcgc ctgctgactg aagtgatgat gacgcagcat ctctgtgcgt
    23821 gctctttcat aaaaagcgac gtaactggcg tggtacacca caccaccggc atcggtatct
    23881 tcatagtaga cgcgaaccgg ccatcgaaac agcgttgtat tcactttaca tcccggtaat
    23941 gcaacaaaag ttagagcttt taaacgctgc tactatacgc gcgcgattga ggtttgggaa
    24001 tgagggcaag ttaagggcgc gtaaaaattt atgggcctgg agcggcccat aattttttaa
    24061 agagaattct gatttaaaag aagaagaaaa tcagcccgac aatgagaaca atatcggcaa
    24121 gcaatgggca aaaaatccct tgccaaagaa ccttctgcgg acgaaagccc acgccgtgaa
    24181 tcacaccagc acagacggcc cacatcagca ataaaccatg ccagatttcc agttcactgg
    24241 tcttcgcggc aaaacgagat gggtcccaaa acatacatcc tgctaacaga agcgccatca
    24301 cgaaggaaag cgcccgcagg gggcgcttgt ccattaccgc atacaaggtc gcgataatct
    24361 tactcatcag atgtcttctt gaccggcttt gcctgattcg acgtgttcaa gcgccagcgc
    24421 ggtgattacc ccaaacgaac aggcaagaag cgttcccaga atccatgcga aataccacat
    24481 ttttagctcc ttacttagta cagagagtgg gtgttacgtt caatatcttc tttggtgata
    24541 cgaccgaaca ttttccagta acaccaggcg gtgtagagca gaatgatcgg taccagaacc
    24601 accgcaaccc aggtcatgac gttaagcgtc agctggctgg aagttgcatc ccacattgtc
    24661 agacttgcgt tcatcatggt gctggacggc atcacaaacg ggaacattgc gataccggct
    24721 gtcaggatga tgcaggccag cgtcagggag gagaacacaa acgcccacgc ggctttatcc
    24781 atacgtgcag tcaggatggt cagcagcagc agaaccacac ccagtgccgg aatagcccac
    24841 agaattggcg tgttgttgaa gttaaccagc catgcgccag cttcacgaac cacttcttta
    24901 ttcagtgggt tagaggctgc gtaatggtcc attgtcgatt tcacgacata accatcgata
    24961 ccgtacatca cccatacgcc agccagtgcg aaacagacca gtgtcaccag cgcagccacc
    25021 tgagccgttg cacgggtacg caggtgcagt tcgcccacgg tacgcatttg cagataggtt
    25081 gcgccctgag taatgatcat ccctacgctc accacgcctg ccagcaggcc gaacgggtta
    25141 agcaactgga agaagttacc ggtgtagtac agacgcagat attcatcaac gttgaacggt
    25201 acgccctgca acaggttacc gaacgctaca ccaattacca gcggcggaac gaagctacca
    25261 atgaagatgc cccagtccca catgttacgc cagcgggttt cttcaatctt ggagcggtag
    25321 tcaaaaccga ccggacggaa gaacaaagac gccagcacga ggatcatcgc cacatagaag
    25381 ccggagaacg cagcggcata gaccatcggc caggcagcaa agagtgcgcc gcccgcggtg
    25441 atcagccaaa cctggttacc gtcccagtgt ggtgcaatgg agttaatcat aattcgacgc
    25501 tcggtgtcgt tacgaccgag gaaacgggtg agcatgccca cccccatgtc gaaaccgtca
    25561 gtgactgcaa aaccaatcag cagaacgcca accagcagcc accagataaa acgcaatact
    25621 tcataatcga tcatttgacg actcctgtct tagcgtgccg gctgagtagt cgtggaagac
    25681 tgctcaaagt gatagcgacc ggttttcagg ctgcttgggc cgaggcgtgc aaacttgaac
    25741 attaagaaca attctgccac caggaacagg gtatacaggc cgcaaatcag caccattgag
    25801 aagatgagat cgcctgcggt cagtgacgaa ttcgccacag ctgtcggcag cacttcaccg
    25861 atagcccacg gttggcggcc atattcagcc acgaaccagc ccgcttctac agcaatccac
    25921 ggcagcggaa taccgtacag cgcggcgcgc agaagccatt ttttctcgcc aatgcggttg
    25981 cggatgacac tccagaaaga gagcgcgatg attgccagca gcaggaagcc acacgccacc
    26041 atgatacgga acgcaaagta cagcggcgct acacgcggga tggagtcttt ggttgcctgt
    26101 tgaatctgcg cttcagtcgc atcagccacg tttggcgtat agcgtttcag cagcagaccg
    26161 taaccgaggt ctttcttcat gctattgaac tggtcacgaa ccgcctggtc ggtagaacca
    26221 gaacgcagtt gttcgagcag agagtacgcc ttcatcccgt tacgaatgcg ttcttcatgc
    26281 tgcaccatca gctctttcag gccgataacc ggggtatcca cggaacgcgt tgcaatgatg
    26341 cccagtgcgt aagggatctg aatcgcaaat ttgttcgtct cctcttcctg atcaggaatg
    26401 ccgaacaaag taaaggcagc aggcgcaggt tgcgtttccc actcggcttc aatagcagcc
    26461 agtttggttt tctgcacgtc gcccatttcg tagccggatt catcacccag aacaataaca
    26521 gacagaacag cagccatacc gaagctggca gcgatagcaa aggagcgttt agcgaaggcg
    26581 aagtcacgac ctttcagcat ataccatgcg ctgataccga ggatgaacat cgcgccagtc
    26641 acataaccag acgctacagt gtgaacgaat ttcacctgag caaccgggtt aagcaccagc
    26701 tcggagaagc tcaccatctc catacgcata gtttcaaagt tgaaatcgga cgcgattggg
    26761 ttttgcatcc agccgttcgc aaccagaatc cacagtgcgg acaggtttga accgagcgcc
    26821 accagccagg tgacacacat atgctgaact ttacccagac gatcccaacc gaagaagaac
    26881 agacctacaa aggtggattc gaggaagaag gccatcagac cttcgattgc cagcggcgca
    26941 ccgaagatat cccctacata gtgggaatag taagaccagt tagtcccgaa ctggaactcc
    27001 atggtcagac cggtagccac acccagagcg aagttgatac caaacaactt gccccagaac
    27061 ttggtcatat ctttataaat ctgtttgccg gagaggacgt agaccgtttc cataatggcc
    27121 aggaggaacg ccataccgag cgtcagtggc acaaaaagga agtggtacat cgcggtcaag
    27181 gcaaactgta agcgcgacag ttcgactata tctaacatca tgactccttg ctcatcgcat
    27241 gaagactccg agagtgaacc ccgttagaaa gggtcacacg catgccccaa tagaaatttg
    27301 tttgaacact ttcatcatac catgtgaatg cacacaaaaa cggcgtaaag aaaggttgca
    27361 aacatgttaa taaaaactca aattgatccc acgtatatat tacgccgcaa aatccttaca
    27421 ataaacaggt ttttgttgaa gcaaatttgc atttttcgac ggtgatcaat ttatagctaa
    27481 attgccgcct ttcagccaat ttgatcgaga acaatttatc tctttttgat gcccatttcc
    27541 aagacttata cattgataaa tatcaatttc tacaaattat gttaatcatg tttcaacaag
    27601 gtaactttag tgatatttat gtttaaaatg tgtgaattat aagactgaaa ttagtttcgg
    27661 ttggcaatta aagataaaaa gagacgaagc aaaaacagca cggttgacct taattctttt
    27721 tttggtcact ggctaatacg acgcagagtt tcaggattat taataaaggc ataaatctcc
    27781 cccccgcc
//
LOCUS       DAEEUE010000050        27590 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.050, whole genome shotgun sequence.
ACCESSION   DAEEUE010000050 DAEEUE010000000
VERSION     DAEEUE010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27590
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.050"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="KLK84_002274"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="KLK84_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HBE2009579.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="KLK84_002275"
     CDS             complement(1614..1937)
                     /locus_tag="KLK84_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HBE2009580.1"
                     /translation="MRILFVCSLLLLSGCSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="KLK84_002276"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="KLK84_002276"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HBE2009581.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="KLK84_002277"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="KLK84_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HBE2009582.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSYARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="KLK84_002278"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="KLK84_002278"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HBE2009583.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="KLK84_002279"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="KLK84_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HBE2009584.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="KLK84_002280"
     CDS             7537..8574
                     /locus_tag="KLK84_002280"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBE2009585.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="KLK84_002281"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="KLK84_002281"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBE2009586.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="KLK84_002282"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="KLK84_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HBE2009587.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="KLK84_002283"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="KLK84_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HBE2009588.1"
                     /translation="MTPILLSAFWTYTLITAMTPRPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11615
                     /locus_tag="KLK84_002284"
                     /pseudo
     CDS             10745..11615
                     /locus_tag="KLK84_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(11650..12984)
                     /gene="srmB"
                     /locus_tag="KLK84_002285"
     CDS             complement(11650..12984)
                     /gene="srmB"
                     /locus_tag="KLK84_002285"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HBE2009589.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13116..13853
                     /gene="trmN"
                     /locus_tag="KLK84_002286"
     CDS             13116..13853
                     /gene="trmN"
                     /locus_tag="KLK84_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HBE2009590.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13838..15460)
                     /gene="nadB"
                     /locus_tag="KLK84_002287"
                     /pseudo
     CDS             complement(13838..15460)
                     /gene="nadB"
                     /locus_tag="KLK84_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15716..15871
                     /gene="rseD"
                     /locus_tag="KLK84_002288"
     CDS             15716..15871
                     /gene="rseD"
                     /locus_tag="KLK84_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HBE2009591.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15868..16443
                     /gene="rpoE"
                     /locus_tag="KLK84_002289"
     CDS             15868..16443
                     /gene="rpoE"
                     /locus_tag="KLK84_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HBE2009592.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16476..17126
                     /gene="rseA"
                     /locus_tag="KLK84_002290"
     CDS             16476..17126
                     /gene="rseA"
                     /locus_tag="KLK84_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HBE2009593.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17126..18082
                     /gene="rseB"
                     /locus_tag="KLK84_002291"
     CDS             17126..18082
                     /gene="rseB"
                     /locus_tag="KLK84_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HBE2009594.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18079..18558
                     /gene="rseC"
                     /locus_tag="KLK84_002292"
     CDS             18079..18558
                     /gene="rseC"
                     /locus_tag="KLK84_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HBE2009595.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18756..20555
                     /gene="lepA"
                     /locus_tag="KLK84_002293"
     CDS             18756..20555
                     /gene="lepA"
                     /locus_tag="KLK84_002293"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBE2009596.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20571..21545
                     /gene="lepB"
                     /locus_tag="KLK84_002294"
     CDS             20571..21545
                     /gene="lepB"
                     /locus_tag="KLK84_002294"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBE2009597.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21817..22497
                     /gene="rnc"
                     /locus_tag="KLK84_002295"
     CDS             21817..22497
                     /gene="rnc"
                     /locus_tag="KLK84_002295"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBE2009598.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22494..23399
                     /gene="era"
                     /locus_tag="KLK84_002296"
     CDS             22494..23399
                     /gene="era"
                     /locus_tag="KLK84_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBE2009599.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23411..24139
                     /gene="recO"
                     /locus_tag="KLK84_002297"
     CDS             23411..24139
                     /gene="recO"
                     /locus_tag="KLK84_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBE2009600.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24151..24882
                     /gene="pdxJ"
                     /locus_tag="KLK84_002298"
     CDS             24151..24882
                     /gene="pdxJ"
                     /locus_tag="KLK84_002298"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HBE2009601.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24882..25262
                     /locus_tag="KLK84_002299"
     CDS             24882..25262
                     /locus_tag="KLK84_002299"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBE2009602.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25682..25762
                     /gene="shoB"
                     /locus_tag="KLK84_002300"
     CDS             25682..25762
                     /gene="shoB"
                     /locus_tag="KLK84_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HBE2009603.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25956..26216)
                     /gene="yfhL"
                     /locus_tag="KLK84_002301"
     CDS             complement(25956..26216)
                     /gene="yfhL"
                     /locus_tag="KLK84_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HBE2009604.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26431..27527
                     /locus_tag="KLK84_002302"
                     /pseudo
     CDS             26431..27527
                     /locus_tag="KLK84_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6805 a         6865 c         7230 g         6690 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccggcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcatccag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 acgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtagctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatca
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cttggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgttgctgcc gcacatttat gaacttaccc gagtgatgga tacgcttcgt gaggctgcca
    10981 aaaaggagtc agatccggac ggggaactac gcgttgtttc gggcgaaacg ttgctctctt
    11041 accgaatgcc gcaggtgttg cagcgttttc ggcaacgcgc accaaaagtg cgtttatcgt
    11101 tgcaggcgct gaactgttac gtgatccgtg atgcgctact gaatgatgag gccgatgtcg
    11161 gcgtctttta tcgcgtaggg aatgatgatg ccctgaaccg acgagagttg ggtgaacaat
    11221 cgctggtgct ggtggcttca ccacaaattg cagatgtcga ttttactgag ccgggaagac
    11281 ataacgcctg tagctttatt atcaacgaac cgcaatgtgt cttccggcag atatttgaga
    11341 gcacgctgcg acagcggcgg atcacggtgg aaaacaccat tgagcttata agtatcgaaa
    11401 gcatcaagcg ttgtgtcgcg gcgaatatcg gtgtcagtta cttgccccac tttgcggtgg
    11461 aaaaagagat tgagagcgga gaattaattg aattgccttt tggcgaacag tcgcagacca
    11521 ttacggcaat gtgcgcacat tatgctggaa aagcggttag cccggcgatg cacattttta
    11581 ttcagtgtgt tgaagagtgt tttgtggttg cgtaaaaaaa caatgccggg cttaaacccg
    11641 gcattgagat tactcttctg tcgtttgcgg tggcacgccc gttccgctcg gtttacggcg
    11701 cttaccaata tttttggtgt cgcgatggcg ttttttcacc cgcggcttct ctttctcttt
    11761 agcttttttc ttctcggcgc gttttgccag tactttcttc gatggcttgc cggtctgctt
    11821 ctcgcttggc gcacgcgttt tcgggcgtaa ctcatcaata acgcgagctt taatcggctc
    11881 ttcaatgtaa cggccaacct tgcccagcag caaatggtca tgggcttcca ccagcgaaat
    11941 tgcggtgcct ttacgaccgg cgcgcgcggt acgtccgata cgatgcaaat aagtatcgcc
    12001 actgcgcggc atatcgaagt taaagacgtg gctgacgtca ggaatgtcga taccgcgcgc
    12061 ggcaacatcg gtggcaacca gtacgtttac gcggccttcg gtcaggcgtt taattgcttc
    12121 gttacgcttg ccttgtacca tctcaccttc gagatagcag ttgttgatgc ccgcttcgcg
    12181 cagccagttt gccagctcat gcacacgttc acgcttacgc acaaacacga ttgagcgagt
    12241 cgcttcaggc tgttttaaca ggtgcaccag caatgcggtt ttatgttcca ggtcatcggc
    12301 gcggtaatac cactgatgaa tttttttgcg ctcacgggtg gacggattgg cagaaacttc
    12361 caccggatct tccagcagac gctcggcaaa gtcctgaatc gcatcgcctt ccagcgttgc
    12421 cgaaaagagc agggtctgtt tacgccagcg agtttcgcca gcaatatgtt cgatatcctg
    12481 agcgaagccc atatccagca tacggtctgc ttcgtcgagg atcagcgttt caaccgcgcg
    12541 gcaatcgaag ttctcttctt ttatgtattg cagcagacgt ccggtcgtgg cgaccacgat
    12601 gtcctgattt tcgctgaaca cttccgcgtg gttcatatag gctacgccgc cggtgatggt
    12661 ggcgatatcc agatgcgtat gtttcgccag ttcgcgggca tgatcggcca cctgcatcgc
    12721 cagctcgcga gttggggtga ggatcaaaat acgcggcgga ccggatttct tacgtgggaa
    12781 atcgagcagg tgctgcaacg ctggcagcag atacgccgcc gttttaccgg tgcctgtcgg
    12841 cgcagaaccg agtacatcac ggccatcgag cgcaggcgga atggcggcag cctgaatggc
    12901 ggtcgggcga gtgaaacctt tatcctggag ggcttccagc aggctttcgt cgagttcaag
    12961 ttcggaaaaa gtcgttacag tcatgttcta cctctgtgtg gggcgctgat tatagacgtt
    13021 acggctgcaa tcttcatctg tttgtatgga tatcgcttct cgggtattgt tctcacccgg
    13081 tgctatcgcc gtttttcctg caaggtttta ttttcatgtc acagtctaca tccgtgcttc
    13141 gtcgtaatgg atttactttt aaacagtttt ttgttgctca cgatcgctgt gcgatgaaag
    13201 tgggaacgga tggcattttg ctgggcgcat gggcaccggt ggctggggtg aaacgttgcc
    13261 ttgatatcgg cgcgggtagc gggttgctgg cactgatgct ggcgcagcga accagtgaca
    13321 gcgtgataat tgatgccgtt gagctggaaa gtgaagctgc cgcgcaggcg caggaaaata
    13381 tcaaccagtc cccgtgggca gagcggatta acgtccatac ggcggatatt cagcagtgga
    13441 tcacacagca gacagtacgc ttcgatttaa tcatcagtaa cccaccttac tatcagcagg
    13501 gagtagagtg cgcgacacct caacgggaac aggctcgcta taccactacg cttgatcacc
    13561 catcgctgct gacctgtgcg gcggagtgca ttaccgaaga gggatttttc tgcgtggtgc
    13621 tgccagagca gattggtaat ggttttactg aactggcatt aagcatgggc tggcatttac
    13681 gtttgcggac ggatgtggcg gaaaacgaag cgcgactgcc gcatcgggtg ctgctggcat
    13741 tctccccgca ggcgggagaa tgctttagcg atcgcttagt cattcgtggg ccagaccaga
    13801 actattccga agcgtatacg gcgctgaccc aggcttttta tctgtttatg taatgattgc
    13861 cgggggaaag gatcgatgga ccggaatgcg tgagcagttc cgggtaatcc agcgtaaaat
    13921 gcagcccccg gctttctttg cgcatcatcg cgcagcgaac aatcaactcg gcaacctgta
    13981 ccagattacg cagctccagc aaattatttg agacgcggaa atgggcgtaa tattcgtcta
    14041 tttcttgttg gagcatggtt atccgccgca gggcgcgttc caggcgcttc gttgtgcgca
    14101 caatgccaac gtaatcccac ataaacagcc gtagctcgtg ccagttatgc tgaattacta
    14161 cccgttcgtc agggttctca acgcggcttt catcccacgg cggtaacgta ctgacgccgt
    14221 gggcataagg catacgtctg gtgatatctt ctgccgccga ccagccatag accagacact
    14281 ccagcaatga attcgaggcc atgcggttag cgccgtgtaa gccggtataa ctcacctcgc
    14341 caatggcata caagccctcg acgtccgtac gcccatgatc atcaaccatt acaccacctc
    14401 aggtatagtg tgcagcaggc acaatcggta ccggttcttg tgtgagatca atccccagcc
    14461 cgagcaattt ttcataaatc atcgggaaat gctggcgaat aaaatcggcg ggcttatggc
    14521 tgatgtcgag gaacatacaa tctgcgccga ggcgtttcat ttcatggtca atggcgcggg
    14581 cgacaatatc gcgcggggcc agttcgccgc gctcatcaaa atcgggcata aaacgcgtgc
    14641 catccgggcg cttgagataa gcgccttcgc cgcgcagtgc ttccgttaac aggaaattgc
    14701 gtgcctgtgg gtgatacagt gcggtagggt ggaactgatt aaattcgaga ttggcaaccc
    14761 gacagcccgc gcgccacgcc atagcaatgc catcgccaga agaaatatcc ggattggtgg
    14821 tgtactgata caccttcgac gcaccgccgg ttgccagtac cactgctttt gcgtggcagg
    14881 tttccaccgt ttctttattt cggttccata cccacgcgcc tacaacccgt cgcgtgcccg
    14941 gcaggccaat tttgtcagag acaatcagat caactgcgtt gctgcgctcc agcacacgaa
    15001 tattcggatg gttctgcgcc ttgctcacca gcgtggattg tacttctcta ccggtggcgt
    15061 cggcggcatg aagaatacga cggtgactat gtccaccttc acgggtcaga tggtagcttt
    15121 cttcgccatt cggttgaacg tgggtatcaa acaaaacccc ctggtcaatt aaccatggca
    15181 cacaggaacg agcattgctg gcgacaaatt caactgcatg gtgatcgcaa ataccagccc
    15241 cggcaatcaa tgtgtcttcc acatgcgagt caatgctgtc agtttcatca aacacggcgg
    15301 caataccgcc ctgggcataa aatgttgaac cttccgttac cgggccttta cttagaacga
    15361 tgacctgatg ctggtcagcc aggcgtagcg ccagtgaaag tccggctgcg ccgctaccaa
    15421 taatcaacac gtcacatgaa tgttcaggga gagtattcat tttctttgtt taatttacta
    15481 aacattgttt ggtcagcata gcatcatgtt gtgcgaataa acacctgcta ttttaatatt
    15541 tgttacagtg gctaaacacg ttgacgcagg gcggcgagaa aaagagaagt tactggctgg
    15601 tggaggatta ggtggtgaaa taaaaaggcc gttgggttac tcttcaggca gttaaatggg
    15661 catttctaca cagataatgc gatgttcaga ttctgtagac ttataatgat agataatgat
    15721 ccgtctacag catgacaaac aaaaacagat gcgttacgga actttacaaa aacgagacac
    15781 tctaaccctt tgcttgctca aattgcagct aatggagtgg cgtttcgata gcgcgtggaa
    15841 atttggtttg gggagacttt acctcggatg agcgagcagt taacggacca ggtcctggtt
    15901 gaacgggtcc agaagggaga tcagaaagcc tttaacttac tggtagtgcg ctatcagcat
    15961 aaagtggcga gtctggtttc ccgctatgtg ccgtcgggtg atgttcccga tgtggtacaa
    16021 gaagctttta ttaaagccta tcgtgcgctg gattcgttcc ggggagatag cgctttttat
    16081 acatggctgt atcggattgc tgtaaataca gcgaaaaatt acctggttgc tcaggggcgt
    16141 cgtccacctt ccagtgatgt ggatgccatt gaagctgaaa acttcgaaag tggcggcgcg
    16201 ttgaaagaaa tttcgaaccc tgagaactta atgttgtcag aagaactgag acagatagtt
    16261 ttccgaacta ttgagtccct cccggaagat ttgcgcatgg caataacctt gcgggagctg
    16321 gatggcctga gctatgaaga gatagccgct atcatggatt gtccggtagg tacggtgcgt
    16381 tcacgtatct tccgagcgag ggaagctatt gataacaaag ttcaaccgct tatcaggcgt
    16441 tgacgatagc gggatactgg ataagggtat taggcatgca gaaagaacaa ctttccgctt
    16501 taatggatgg cgaaacgctg gatagtgagc tgcttaacga actggctcat aacccagaaa
    16561 tgcagaaaac ctgggaaagc tatcacttaa tccgtgattc aatgcggggt gatactcccg
    16621 aggtgctcca tttcgatatc tcttcacgcg tgatggccgc cattgaagaa gagccagtac
    16681 gtcaatcggc gacattgatc ccggaagccc agcctgcgcc gcatcaatgg cagaaaatgc
    16741 cattctggca gaaagtacgt ccgtgggcgg cacagcttac ccaaatgggc gtagccgcat
    16801 gcgtatcgct tgcagttatc gttggcgtcc agcactataa tggacaatct gaaacgtccc
    16861 agcagcccga aacgccggta tttaatacac tgccgatgat gggtaaagcc agcccggtaa
    16921 gcctgggagt accttctgaa gcgaccgcaa acaatggtca acagcagcag gtacaggagc
    16981 agcgtcgtcg cattaatgca atgttgcagg attacgaact gcaacgccga ctccactctg
    17041 aacagcttca gtttgagcag gcgcaaaccc agcaagccgc tgtacaggtg ccaggaattc
    17101 aaactttagg aacgcaatcg cagtaatgaa gcaactttgg tttgccatgt cattagtgac
    17161 aggtagcctg ttattctctg ctaacgcctc ggccactccc gcgtccgggg cgttattaca
    17221 gcagatgaac ctggccagtc agtcactgaa ttacgagctg tcattcatca gcatcaataa
    17281 acagggtgtt gagtctctgc gttatcgaca tgcacgcctc gataaccgtc ctcttgcaca
    17341 attgttgcaa atggatggcc cgcgccggga agtggtacag cgcggcaatg aaatcagcta
    17401 ttttgaaccg ggacttgaac cgttcacgct taatggcgat tacattgttg attctctgcc
    17461 atcgcttatc tataccgatt tcaaacgcct ttctccttac tacgacttta tctccgtcgg
    17521 gcgcacgcgt attgctgatc gtctttgcga agtcattcgc gtggttgccc gagatggtac
    17581 acgctacagc tacatcgtgt ggatggacac cgaatcgaaa ttaccgatgc gggttgatct
    17641 ccttgatcgc gatggtgaaa cgctggaaca atttcgcgtg attgctttta acgtcaatca
    17701 ggatatcagc agcagtatgc agacgctggc gaaggcaaat ttgccgccgt tgctttctgt
    17761 tcctgtaggt gaaaaagcta aattcagctg gacgccaacc tggttgccac agggttttag
    17821 cgaagtttcc agtagtcgac gtccgctacc gacgatggac aacatgccta tcgaatcacg
    17881 tctctattcc gacggattat tcagcttctc ggtaaacgtt aaccgcgcta cgccatcgag
    17941 caccgatcag atgttgcgca ccggacgcag aaccgtcagt acaagcgtac gtgataacgc
    18001 cgaaatcacc attgtcggtg aactgccgcc gcaaacggcg aaacgcattg ccgagaatat
    18061 taagttcggg gcagcgcaat gatcaaagag tgggctaccg tcgtctcctg gcaaaacggg
    18121 caggcgctgg tcagttgtga tgttaaagct tcatgcagca gctgcgcttc acgcgccggt
    18181 tgcggtagcc gcgtgttgaa taaacttggc ccgcaaacca cgcataccat tgtcgtaccc
    18241 tgtgatgagc cgttagtgcc ggggcaaaaa gtggaattgg ggatcgccga aggcagcctg
    18301 cttagctccg cattactggt ttatatgtcg ccgctggtgg gattattcct catcgcttcg
    18361 ctatttcagc tactctttgc ttctgacgtc gcagcattat gtggtgcggt cctcggcggt
    18421 attggcgggt tcctgattgc gcgcggctac tcgcgtaagt ttgctgcccg ggcagaatgg
    18481 caaccgatca tcttaagcgt ggcattgccg cctggccttg tgcgatttga aacatcttcg
    18541 gaagacgcga gccagtgatt tcactggctt tgttgcattt tgcataacaa atgcgaggac
    18601 gtttccagat accacaatcc atacggtatg aacatttcct cgcctcaatg ttgtagtgta
    18661 gaatgcggcg tttctattaa tacagacgtt aagctcagaa cagcgacttc taaaagcctg
    18721 gttaaccggg cattaaggca caataatcat actttatgaa gaatatacgt aacttttcga
    18781 tcatagctca cattgaccac ggtaaatcga cgctgtctga ccgtattatc cagatctgcg
    18841 gtggcctgtc tgaccgtgaa atggaggcgc aggttctcga ttccatggat cttgagcgtg
    18901 agcgtggcat taccatcaaa gcgcaaagcg tgacgctgga ctacaaagcg tctgacggcg
    18961 aaacctatca gcttaacttt atcgacaccc cgggccacgt agacttctcc tatgaagttt
    19021 cccgttcgct ggctgcctgt gaaggtgcat tgctggtggt cgacgccggg cagggcgtag
    19081 aagcgcaaac cctggcaaac tgctacaccg ccatggaaat ggatctcgaa gtggtgccgg
    19141 tactgaacaa gattgacctg ccggcagccg atcctgaacg cgtggcggaa gaaattgaag
    19201 atatcgtcgg catcgacgcc accgatgcag tgcgctgttc agcgaaaacc ggcgttggcg
    19261 tgcaggacgt tctcgaacgt ctggtgcgcg acattccgcc gccggaaggc gatccggaag
    19321 gcccgttgca ggcactaatt atcgactcct ggttcgacaa ctacctgggc gttgtttcac
    19381 ttatccgtat taaaaacggc accctgcgta agggcgacaa agtgaaagtc atgagtaccg
    19441 ggcagaccta taacgccgac cgtctgggca tcttcacgcc gaaacaggtt gaccgcactg
    19501 aactgaaatg tggtgaagta ggctggctcg tatgtgcgat taaagatatc cacggcgctc
    19561 cagtcggcga taccttaacg ctggcgcgta atccggcaga aaaggcgctg cctggcttta
    19621 agaaagtcaa accgcaggta tacgccggtc tgttcccggt aagttccgac gactatgaag
    19681 ccttccgtga cgcgctgggt aaactcagcc tgaacgatgc ctcactgttc tatgagccgg
    19741 aaagctccag cgcgctgggc tttggtttcc gctgcggctt ccttggcctg ctgcacatgg
    19801 agatcatcca ggagcgtctg gaacgtgaat acgatctgga tctgatcacc actgcgccga
    19861 ccgtagtgta tgaagttgaa accacgtcaa gggaagttat ctacgtcgac agcccatcca
    19921 agctgcctgc ggtaaataac atttacgaac tgcgcgagcc gattgcagag tgtcacatgc
    19981 tgctgccgca ggcatatctc ggcaacgtta ttacgctgtg cgtagaaaaa cgcggcgtgc
    20041 agaccaatat ggtttaccac ggtaatcagg tggcgctgac gtacgagatc ccgatggcgg
    20101 aagtggtgct cgacttcttc gatcgcctga aatctacctc gcgtggttat gcgtctctgg
    20161 attacaactt caaacgcttc caggcgtccg acatggtacg tgtagacgta ttaatcaacg
    20221 gtgaacgtgt tgatgcgctg gcgttgatca cccaccgtga taattcgcaa aaccgaggtc
    20281 gcgagttggt ggagaagatg aaagatctga tcccacgcca gcagtttgat atcgccattc
    20341 aggcagcgat tggtacgcac attattgcgc gatccaccgt gaaacagctg cgtaaaaacg
    20401 tactggctaa atgttatggc ggcgatatca gccgtaagaa aaagctgctg cagaagcaga
    20461 aagaaggtaa gaaacgcatg aagcagatcg gtaacgttga gctgccgcag gaagcgttcc
    20521 tcgccattct gcacgtcggc aaagacaaca aataaccctt aggagttggc atggcgaata
    20581 tgtttgccct gattctggtg attgccacac tggtgacggg cattttatgg tgcgtggata
    20641 aattcttttt cgcacctaaa cggcgggaac gtcaggcagc ggcgcaggcg gctgccggtg
    20701 actcactgga taaagcaacg ttgaaaaagg ttgcaccgaa gcctggctgg ctggaaaccg
    20761 gagcttctgt ttttccggtg ctggctatcg tattgattgt acgttcgttt atttatgaac
    20821 cgttccagat cccgtcaggt tcgatgatgc cgactctgtt aatcggtgat tttattctgg
    20881 tagagaagtt tgcttatggc attaaagatc ctatctacca gaaaacgctg atcgaaaccg
    20941 gtcatccgaa acgcggcgat atcgtggtct ttaaatatcc ggaagatcca aagcttgatt
    21001 acatcaagcg cgcggtgggt ttaccgggcg ataaagtcac ttacgatccg gtctcaaaag
    21061 agctgacgat tcaaccggga tgcagttccg gccaggcgtg tgaaaacgcg ctgccggtca
    21121 cctactcaaa cgtggaaccg agcgatttcg ttcagacctt ctcacgccgt aatggtgggg
    21181 aagcgaccag cggattcttt gaagtgccga aaaacgaaac caaagaaaat ggaattcgtc
    21241 tttccgagcg taaagagaca ctgggtgatg tgacgcaccg aattctgaca gtgccgattg
    21301 cgcaggacca ggtggggatg tattaccagc agccagggca acaactggca acctggattg
    21361 ttccgccggg acaatacttc atgatgggcg acaaccgcga caacagcgcg gacagccgtt
    21421 actggggctt tgtgcctgaa gcgaatctgg tcggtcgggc cacggctatc tggatgagct
    21481 tcgataagca agaaggcgaa tggccgactg gtgtgcgctt aagtcgcatt ggcggcatcc
    21541 attaatagcc atcttcgttc acgttgtcgc cgttatggcg acaacgtgaa ttatttatga
    21601 gataaatctc ccgtggctaa cgacatcccc cgtcgttgtg tatagaatat tcccccgaag
    21661 tttaaggttg gcacctccag gttgccacgg cacacgaaac agcgttggtc cccatatacc
    21721 gctaaactga aactgcagcg aagcagttag cagagccatg tatatcaggt ctgtttcgtg
    21781 tgctgaattg ttgacgcatt tatttattgg tatcgcatga accccatcgt aattaatcgg
    21841 cttcaacgga agctgggcta cacttttaat catcaggaac tgttgcagca ggcattaact
    21901 catcgtagtg ccagcagcaa acataacgag cgtttagaat ttttaggcga ctctattctg
    21961 agctacgtta tcgccaatgc gctttatcac cgtttccctc gtgtggatga aggcgatatg
    22021 agccggatgc gcgccacgct ggtccgtggc aatacgctgg cggaactggc gcgcgaattt
    22081 gagttaggcg agtgcttacg tttagggcca ggtgaactta aaagcggtgg atttcgtcgt
    22141 gagtcaattc tcgccgacac cgtcgaagca ttaattggtg gcgtattcct cgacagtgat
    22201 attcaaaccg tcgagaaatt aatccttaac tggtatcaaa ctcgtctgga cgaaattagc
    22261 ccaggcgata aacaaaaaga tccgaaaacg cgcttgcaag aatatttgca gggtcgccat
    22321 ctgccgctgc cgacttatct ggtagtccag gtacgtggcg aagcgcacga tcaggaattt
    22381 actatccact gccaggtcag cggcctgagt gaaccggtgg ttggcacagg ttcaagccgt
    22441 cgtaaggctg agcaggctgc cgccgaacag gcgttgaaaa aactggagct ggaatgagca
    22501 tcgataaaag ttactgcgga tttattgcca tcgtcggacg tccgaacgtt ggcaaatcca
    22561 cattgttgaa caaactgctg gggcagaaaa tctccatcac ctcccgcaag gcgcagacaa
    22621 ctcgtcaccg cattgtgggg atccatactg aaggcgcgta tcaggcgatc tacgtcgata
    22681 caccgggcct gcatatggaa gaaaaacgcg ccattaaccg cctgatgaac aaagcggcga
    22741 gcagctctat tggcgatgtt gagctggtga tttttgtcgt tgaaggcacc cgctggacgc
    22801 cggacgacga aatggtgctc aacaaactgc gcgaaggcaa agcgccggta atcctcgcgg
    22861 tgaacaaagt ggacaacgtg caggagaaag ccgatctgct gccgcacctg cagttcctgg
    22921 caagccagat gaacttcctc gatatcgtgc caatctctgc cgaaaccggg ctgaatgttg
    22981 acactattgc ggcaatcgtg cgtaagcatc tgcctgaagc gactcatcac ttcccggaag
    23041 attacatcac cgatcgctca cagcgtttta tggcgtctga aatcatccgc gaaaaactga
    23101 tgcgtttcct tggcgctgaa ctgccgtact ccgtgaccgt ggagatcgaa cgtttcgtct
    23161 ctaacgaacg cggcggttgt gacatcaacg gtttgattct cgttgagcgt gaagggcaga
    23221 agaagatggt tattggcaac aaaggggcca agatcaaaac catcgggatt gaagcgcgta
    23281 aagacatgca ggaaatgttc gaagcgcctg tccaccttga actgtgggta aaagtgaaat
    23341 cgggttgggc cgacgacgaa cgcgcactgc gcagtctcgg ttatgttgac gatctttaag
    23401 agtcactccg atggaaggct ggcagcgcgc atttgtcctg catagtcgcc cgtggagcga
    23461 aaccagcctg atgctggacg tcttcacaga ggaatcgggt cgcgtgcgtc tggttgccaa
    23521 aggcgcacgc tctaaacgct ctactctgaa aggtgcatta cagcctttca cccctctctt
    23581 gctacgtttt ggcgggcgtg gcgaagtcaa aacgctgcgc agtgctgaag ctgtctcact
    23641 ggcgctgcca ttaagcggta tcacgcttta cagcggtctg tacatcaacg aacttctctc
    23701 ccgcgtactg gaatacgaga cgcgcttctc tgaactcttt ttcgattact tgcactgcat
    23761 tcagtctctt gcaggtgaca ctggtacgcc agaacccgcg ctgcgtcgct ttgaactggc
    23821 actactcggg catctgggct atggcgtcaa ttttacccat tgcgcgggta gcggcgagcc
    23881 ggtagatggc accatgacgt atcgttatcg cgaagaaaaa gggtttatcg caagcgtcgt
    23941 tatcgacaat aaaacgttca ccggaaggca gttaaaagcg ttaaacgcgc gggaatttcc
    24001 tgacgcagac acactacgcg ccgcgaaacg ctttacccga atggcgctta agccgtatct
    24061 tggcggtaaa ccattaaaga gcagggaact gttccggcag tttatgccta agcgaacggt
    24121 gaaaacacat tatgaatgat gaggattgtc atggctgaat tactgttagg cgtcaacatt
    24181 gaccatattg caacgctgcg taacgcacgc ggtactgctt acccggatcc ggtgcaggcg
    24241 gcgtttattg ccgagcaggc gggagcggac ggcattaccg tgcatttacg tgaagatcgc
    24301 cgccacatta ctgaccgcga tgtgcgcatc ctgcggcaga cgctggatac ccgaatgaat
    24361 ctggagatgg cagtgaccga agagatgctg gcgatcgccg ttgagacgaa gccacatttt
    24421 tgctgcttgg taccggaaaa gcgtcaggaa gtaacaaccg aaggcggcct ggacgttgca
    24481 gggcagcgtg aaaaaatacg cgatgcctgc aaacgtctgg cagatgccgg gattcaggtt
    24541 tctctgttta tcgacgccga tgaagagcag atcaaagctg cggcagaggt tggcgcaccg
    24601 tttatcgaga tccacaccgg ttgctatgct gatgccaaaa ctgacgccga acaggcgcaa
    24661 gagctggtgc gtatcgccaa agccgcgacc tttgccgcta gtctcggtct gaaagttaac
    24721 gccggacacg gtctgaccta tcacaacgtg aaagccattg ccgccatccc tgagatgcat
    24781 gaactgaata tcggtcatgc cattattggc cgtgcagtga tgaccggact gaaagatgcg
    24841 gtggcagaaa tgaagcgtct gatgctggaa gcgcgtggct aatggcaata ttaggtttag
    24901 gcacggatat cgtagagatc gctcgcatag aagcagtgat cgcccgatcc ggtgagcgcc
    24961 tggcacgccg cgtattaagc gataacgaat gggcaatctg gaaaacgcac caccagccgg
    25021 tacgttttct ggcgaagcgt tttgctgtga aagaagccgc agcaaaagcg tttggcaccg
    25081 ggatccgcaa tggtctggcg tttaatcaat ttgaagtatt caatgatgag ctcggcaaac
    25141 cacggctacg gctatggggt gaggcattaa aactggcgga aaagctcggc gttgtaaata
    25201 tgcatgtaac gctggcagat gaacgccact atgcttgtgc cacggtaatt attgaaagtt
    25261 aaaataatac cgttctcttt aaaaagtaaa acccccgaac tggtaaggaa cgggggtttt
    25321 actttttaac tttaattttg tgcagcatgc accctcaaca acagcgatta tggatcagtg
    25381 tacttatctt ttacaagcaa cttgcaatct ttagcataaa aactcgagcc tttacgaaga
    25441 aagcaatatt gatggaaaga ttaacgtgac cgccaattcg taagtacatt aaaattggct
    25501 tcgttattga agattttgct gtgctttaca ccatgccaca gaattccccc attgaaacga
    25561 gtggtgtcgt caaagctctg gtgtggagtg cagcatgcac cctcaataac tcgcacgttc
    25621 agttttgggg agatgtaagg gctaatctga atggctgcat tccttgttta aggaaaaacg
    25681 aatgactgat tgccgatacc tgattaaacg ggtcatcaaa atcatcattg ctgttttaca
    25741 gctgatcctt ctgttcttat aacacaagga aacgtactta aggtgcgtcc ggtgaaccaa
    25801 tcggacgcgc ctttaataac tataaataag tgtcttggca gatactatat aaattaactt
    25861 agtgaatgat tatgctaatg tcatcaatta aataaatata atggcgttaa ggcttcccag
    25921 taatataatt aatactctac ttccagagta gaatattaaa ttttatccgc gtggtgcatc
    25981 agcacaaatt tatcccacaa ctgttcttct gtctcgacat gcgccggatc tttcacaata
    26041 gtattgggga tcgggcacac cttctggcag gttggtgtct cgtagtgccc tacgcattcg
    26101 gtacacttat cgctgttaat ttcgtagata tgatctccca ttgaaatcgc ctcattcggg
    26161 cattcgggtt cacacatatc acaattaata cagcgtttag taattagtaa agacatttca
    26221 atggattacc gttaaatcat tttaaaatca gtaagttgta tcgagtttgt atgctttact
    26281 gtcattaact tactgtatgt tgatccagtg tatttaacct tgataaactc agtccagcaa
    26341 cacaaaaccg caacacattg cattttgtcc cgtagaaaag acttgtatgt gtgagcttgt
    26401 tttctgcgcc tacgcagata aggattgaga atgccgcgca ctgtaacaca taatccggat
    26461 agccccaata atgacgatgt tttagccgca tctgaaaaat gggacgcctg taaacccccc
    26521 tataccagcg cacacatgaa aatctgtgtt gctgccgcca aaatcatcct cgctgcttcc
    26581 ggcgtggctc gccgttccaa atacgaaaaa gagaactatc tccgtatcga tttcagcaaa
    26641 gccggtaagg ttacatttta cgccgagttt ccaaaaaaga tgggcctcaa aggtaaaaag
    26701 ctcggcgagt ggccggagct cgttatccag ctggcgcgcg aaaaagcact aggtatggct
    26761 gacggtggac tgcgggcaga gtccgtacat gcagcgctgg aaatgtaccg ggatgaccca
    26821 aagccaaagt agcccggcag aagctgagcc cggacagttt cacaacctac ggggtgcgta
    26881 tcgaccggat taaagcaacg ttcggcgagc gtgaggtgtt cagcgacgta acatacaatc
    26941 ggctggtgga agtgctggac gagtggatcg ccactcgctc gaacaataac gccctggagt
    27001 tgtttgccga gctccgtcgg ttctggaagt tctgcgcacc tactctttgc aacggccgca
    27061 atgttgccgc cagtctgcca gatgattatg tttcctcccg cgtacagaaa cctaccccca
    27121 cacggctttt taccgatatt gaatcaatcg cccgactctg gctcaatgtt gctgcctgca
    27181 cctctgtaca ccagaagaat gctgttcgct tcatgatcat cactggtgtt cgtccgatta
    27241 atgtccataa cctgcgctgg gactacgttt acgaggaggc tggtgaaatt gtttatccgg
    27301 aaggggttat cggcatgcga ggggctatga aaacacaaaa ggctttccgc ctgccgataa
    27361 cgcctgagat ccggcggatt atcgacgagc agaaagcctg gcgtgattca gttcctgagt
    27421 gcaacaggga ttatgtattt ttgcagccac gtgatccaat gcagccattt tcaaaacgat
    27481 cactggataa gctggtgaaa acatacagcc cggacggggc tgtaaaatga ggtgtactgg
    27541 caatagcgga cactaccatt tgttcttttt ttaagcagcc atctgatgat
//
LOCUS       DAEEUE010000051        26563 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.051, whole genome shotgun sequence.
ACCESSION   DAEEUE010000051 DAEEUE010000000
VERSION     DAEEUE010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26563
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.051"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            348..641
                     /locus_tag="KLK84_002303"
     CDS             348..641
                     /locus_tag="KLK84_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HBE2009605.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            713..1372
                     /locus_tag="KLK84_002304"
     CDS             713..1372
                     /locus_tag="KLK84_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBE2009606.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1464..1910
                     /locus_tag="KLK84_002305"
     CDS             1464..1910
                     /locus_tag="KLK84_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HBE2009607.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="KLK84_002306"
                     /pseudo
     CDS             complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="KLK84_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3390..3809
                     /locus_tag="KLK84_002307"
     CDS             3390..3809
                     /locus_tag="KLK84_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HBE2009608.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3809..5077
                     /gene="umuC"
                     /locus_tag="KLK84_002308"
     CDS             3809..5077
                     /gene="umuC"
                     /locus_tag="KLK84_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HBE2009609.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5123..5653)
                     /gene="dsbB"
                     /locus_tag="KLK84_002309"
     CDS             complement(5123..5653)
                     /gene="dsbB"
                     /locus_tag="KLK84_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HBE2009610.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5799..7340)
                     /gene="nhaB"
                     /locus_tag="KLK84_002310"
     CDS             complement(5799..7340)
                     /gene="nhaB"
                     /locus_tag="KLK84_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HBE2009611.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHSIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7561..8280
                     /gene="fadR"
                     /locus_tag="KLK84_002311"
     CDS             7561..8280
                     /gene="fadR"
                     /locus_tag="KLK84_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HBE2009612.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8332..9864)
                     /locus_tag="KLK84_002312"
     CDS             complement(8332..9864)
                     /locus_tag="KLK84_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HBE2009613.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10194..11492
                     /gene="dadA"
                     /locus_tag="KLK84_002313"
     CDS             10194..11492
                     /gene="dadA"
                     /locus_tag="KLK84_002313"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HBE2009614.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11502..12572
                     /gene="dadX"
                     /locus_tag="KLK84_002314"
     CDS             11502..12572
                     /gene="dadX"
                     /locus_tag="KLK84_002314"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HBE2009615.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12740..14154)
                     /locus_tag="KLK84_002315"
                     /pseudo
     CDS             complement(12740..14154)
                     /locus_tag="KLK84_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14765..16501)
                     /locus_tag="KLK84_002316"
     CDS             complement(14765..16501)
                     /locus_tag="KLK84_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBE2009616.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16596..17510)
                     /gene="ldcA"
                     /locus_tag="KLK84_002317"
     CDS             complement(16596..17510)
                     /gene="ldcA"
                     /locus_tag="KLK84_002317"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HBE2009617.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17610..18221
                     /gene="emtA"
                     /locus_tag="KLK84_002318"
     CDS             17610..18221
                     /gene="emtA"
                     /locus_tag="KLK84_002318"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HBE2009618.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18223..18957)
                     /locus_tag="KLK84_002319"
     CDS             complement(18223..18957)
                     /locus_tag="KLK84_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HBE2009619.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19158..19412
                     /locus_tag="KLK84_002320"
     CDS             19158..19412
                     /locus_tag="KLK84_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBE2009620.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19462..20922)
                     /locus_tag="KLK84_002321"
     CDS             complement(19462..20922)
                     /locus_tag="KLK84_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HBE2009621.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20859..21416)
                     /locus_tag="KLK84_002322"
     CDS             complement(20859..21416)
                     /locus_tag="KLK84_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HBE2009622.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITPLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21442..22296)
                     /locus_tag="KLK84_002323"
     CDS             complement(21442..22296)
                     /locus_tag="KLK84_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HBE2009623.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22296..23105)
                     /locus_tag="KLK84_002324"
     CDS             complement(22296..23105)
                     /locus_tag="KLK84_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBE2009624.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23115..23873)
                     /locus_tag="KLK84_002325"
     CDS             complement(23115..23873)
                     /locus_tag="KLK84_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE2009625.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23870..24850)
                     /locus_tag="KLK84_002326"
     CDS             complement(23870..24850)
                     /locus_tag="KLK84_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBE2009626.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24850..25872)
                     /locus_tag="KLK84_002327"
     CDS             complement(24850..25872)
                     /locus_tag="KLK84_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HBE2009627.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
BASE COUNT         6683 a         6472 c         6851 g         6557 t
ORIGIN      
        1 tatggccttt gctgaactca acattgacat tcttacccgc agcaaagctc gtggaggtta
       61 gcagtgcaag cagcaataca gccttactga tgctcttgat tttcattggt agtctcctgt
      121 tcagaatgag tacgatgaac tgttataata taacaatccc ttacgggaga attccagtgg
      181 cttttctgcg ttcggtaaaa tcccggcgcg gcattttttg ccggggggat tacttataaa
      241 ggggcatgga ctcaacaaac cgcagaaatc tacagctggc tggcttgtgg cgcgggtttc
      301 attgatgtta gcttatgcca aaacctggca tcttaaagag taaaagtatg ttttgtgtga
      361 tttatcgaag cagcaagcgt gaccagacct atttatatgt cgaaaaaaaa gacgattttt
      421 cgcgtgttcc tgaggaactg atgaaaggtt ttggtcagcc tcagttagcg atgattctgc
      481 cgttggatgg gcggaagaaa ctggtcaatg ctgatattga aaaggttaaa ctggcattaa
      541 ccgagcaagg ttactatttg cagttaccgc caccacccga agatttgctg aagcaacatc
      601 tttccgtcat ggggcagaaa acagacgaca ctaacaaata accgatatcc ggcggtggca
      661 ttatctttgt cggcgcgggt tttcatatcc acgataaggt gaggggaacg ttatgtatca
      721 acatcacaac tggcaaggtg cgctgctgga ttatccggtg agtaaagtag tctgtgttgg
      781 cagtaactat gccaaacata ttaaagagat gggcagcgca gtgcccgaag agccagtgct
      841 gtttattaaa ccagaaacgg cactgtgcga tctgcggcag ccattggcga tcccatccga
      901 tttcggttca gttcatcatg aagtcgaact ggcggtgttg attggcgcga cgctgcgtca
      961 ggcaacggaa gagcatgtcc gcaaagccat tgccggttac ggcgtggcgc ttgatctgac
     1021 attgcgtgat gttcagggaa aaatgaagaa agccgggcag ccgtgggaaa aggctaaagc
     1081 gtttgataac tcttgtccgc tttccgggtt tatccccgcg gcggaattta ccggcgatcc
     1141 acaaaataca acgctgggcc tgagcgtaaa cggcgaaaaa cgccagcaag gtacgactgc
     1201 ggacatgatc cataaaatcg ttccgttgat cgcttatatg agcaagtttt ttaccctcaa
     1261 ggccggtgac gttgtgttga caggcacgcc tgatggcgtc ggcccgttgc aaagcggtga
     1321 tgagctgaca gtcactttcg atgggcattc tttgacaact cgcgttttgt aatacttttt
     1381 gccgccagaa agcggcggca aaacttgcat cgctgtgcca gactggttat aaggtgcgtt
     1441 ttaacgtaat ggcggaacac ctgatgagcg atgtaccttt ctggcaaagt aaaaccctgg
     1501 acgaaatgag cgatgcggaa tgggagtcgt tgtgtgatgg ttgcggtcag tgttgcttgc
     1561 ataaactgat ggatgaagac accgacgaaa tctacttcac taacgtcgcc tgtcgccagc
     1621 tcaatattaa aacctgtcaa tgtcggaact acgaacgtcg tttcgagttt gaacccgact
     1681 gcattaaatt aacccgtgaa aatctgccaa cattcgaatg gctgccaatg acctgcgctt
     1741 atcggttgct ggcggaaggt aaagatttac ctgcgtggca tccgctactt actggttcga
     1801 aagcggcaat gcatggtgaa cgtatctctg tgcgtcatat cgcagtgaaa gaatcagaag
     1861 tgattgactg gcaggatcat atcttaaata aacctgactg ggcgcagtga taaatttaat
     1921 agatgctgat gggggctttc ttaatatacg tctggatgct cgccatttcg tagccctgtt
     1981 tttgaaagcg ctctgtcata atgagagtta cccgatacct tattatcaac aggtaactct
     2041 caatctttaa aaaacgtctg tgtttttaaa tgtgataaac cttatacctt gagtacatgg
     2101 agagaataat cgcttatcag acttcaggta cttcaaagag tgtcttttta ccgtgtcttt
     2161 tctgatactc attacaggtg ttaatcattt ttttggccgc tgcttttagc aaagaaagca
     2221 ttaaatcatc ataatcaaca tagaatctgg ttgtttcagt ttccgttttt atctccccga
     2281 tggcggctat ttcggtggtt aatttcaatt tggcggcatc gatatcttta ttcgcttgtt
     2341 taaccgtgtt agacagggtg gtaaagaaac tctgcacaga ttttaacttg ttcttcaatt
     2401 ctggaatcag tttcccttca actacgcccg cagcaataga ataggaaatg attaaaccaa
     2461 atggaccggc gacgacatcg gctgcggcac cggcatacgc ttccttcctg attttatcta
     2521 cctgtgactg gaaatagctg cttttttctg aaaaatcatt ggttaactgg ctatctaacg
     2581 ccagcagttt cccggaagcg ttgttgaaac tttgtgagct taccagcagg gatttttgcg
     2641 cttcattcag cttcgtgatg ccgtcatcca gtaccttaat gaggggcgga tgctttcttc
     2701 tcattgtact catcaaatag caaaatatac gctgcgagca attgcgtcgc aacaccacac
     2761 cattcataca ctgtttgggt tgcttcaaaa tacttatcct ggctatccat aagtaaggtt
     2821 ttaatatcgc cgactaaaac ggaggctgcc tgtgaatact cctgtttaaa gcgacttaac
     2881 tcttttatgg tttcatcaaa ggtctgccag gggatgacct gatcgagata tttattataa
     2941 agatctaatg ctccatctgc ggtttcgatt gcgtttttaa ctacttctac cgttttatct
     3001 gcaacgattt cagtcataat cattcgcctc tttaaatata taaattgtaa tgaaactcct
     3061 gttttacaac tattaataaa ttttacttca tctaattcat agttagccgg gcgggatgcg
     3121 tcaatgtctt tatttctatt aatatgataa atatcaaaca atgtttaatg tcattatggc
     3181 gaatgcttct attctatttt tttgccgggt gatatttttc atttctgctg gatgagcgtc
     3241 gtcgccagaa ggccacgtga gcacaggata agagaacgaa aaatcagcag tctatgcagc
     3301 gacaaatatt gatagcctga atcagtattg atctgctggc aagaacagac cactgtatat
     3361 aaaaacagta taacttcagg cagattatta tgttgtttat caagcctgcg gatctccgcg
     3421 aaattgtgac ttttccgcta tttagcgatc ttgttcagtg tggctttcct tcaccggcag
     3481 cagattacgt tgaacagcgc atcgatctga atcaactgtt gatccagcat cccagcgcga
     3541 cttacttcgt caaagcaagt ggtgattcta tgattgatgg tggaattagt gacggtgatt
     3601 tactgattgt cgatagcgct attaccgcca gccatggtga tattgtcatc gctgctgttg
     3661 acggcgagtt tacggtgaaa aaattgcaac tacgcccgac ggtacagctt attcccatga
     3721 acagtgcgta ctcgcccatt accatcagta gcgaagatac gctggatgtc tttggtgtgg
     3781 tgatccacgt cgttaaggcg atgcgctgat gtttgccctc tgtgatgtaa acgcgtttta
     3841 tgccagctgt gagacggtgt ttcgccctga tttatggggt aaaccggtgg ttgtgctatc
     3901 gaataatgac ggttgcgtta tcgcccgaaa cgctgaggca aaggcgctgg gcgttaaaat
     3961 gggcgatccc tggttcaaac aaaaagatct gtttcgtcgc tgtggtgtgg tttgctttag
     4021 cagcaattat gagctttacg cagacatgag caatcgggtg atgtcgacgc tggaagaact
     4081 atcgccccgc gtcgagattt acagtattga tgaagcattc tgcgatctga caggtgtgcg
     4141 taattgtcgc gatctgactg attttggcag agaaattcgc gcaacggtgc tacaacgtac
     4201 ccatcttact gttggtgtgg ggatcgccca gaccaaaacg ttggctaagc ttgccaatca
     4261 tgcggcaaaa aaatggcagc ggcagacggg tggggtggtg gatttatcaa atctggaacg
     4321 ccagcgtaaa ttaatgtctg ctctccccgt ggatgacgtc tgggggattg gacggcggat
     4381 cagcaaaaaa ctggacgcga tggggatcaa aaccgttctc gatttggcgg atacagatat
     4441 ccggtttatc cgtaaacatt ttaatgtcat gctcgaaaga acggtgcgtg aactgcgcgg
     4501 cgaaccctgt ttgcaactgg aagagtttgc accgacgaag caggaaatta tctgttcccg
     4561 ctcgtttggt gaacgcatca cggattatac gtcgatgcgg caggccattt gtagttacgc
     4621 tgcccgggcg gcggaaaaac ttcgcagtga gcatcaatat tgtcggttta tctccacatt
     4681 tattaagacg tcaccatttg cgctcaatga accttattac ggcaatagcg cgtcggtaaa
     4741 actgctgacg cccactcagg acagcaggga tatcattaac gctgctacgc gatctctgga
     4801 tgccatctgg caagcgggcc atcgctacca aaaagcgggc gtgatgctgg gggatttctt
     4861 cagtcaggga gtcgcgcagc tcaatttatt cgatgacaac gcaccgcgcc ccgggagtga
     4921 gcaattgatg gcggtaatgg atactctgaa tgctaaagag ggcagaggaa cactctattt
     4981 tgccgggcag gggatccaac aacaatggca gatgaagcga gctatgcttt caccacgtta
     5041 tacaacgcga agttctgatt tactgcgggt caaataaata tagcggcagg aaaaaagcgc
     5101 tcccgcagga gcgccgaatg gattagcgac cgaacagatc acgttttttc gctttaaacg
     5161 gctgggaaat caccaccagc accgcgacaa tcaggtaagc gataaaaata ccgagcagcc
     5221 actgcggcat ttccatacct aaaaaatccc actgacgctc ggcgcaatcg ccagaggcga
     5281 caaacacttg cggcacccat ttatccagcg gcagccattc cgggaaacga accataaaat
     5341 cacaggtggc aaatggcgaa ggatagagct gaagcatggt gtgctcgtaa gttaactgca
     5401 caccgcggaa cgcactatac aaccagataa ccatcgctac ataacgcagc ggagttttcg
     5461 gggcgatcgc gccaatcagc gcagcaccca gaacgccgaa taacgcgcag cgttcataaa
     5521 tacagagcac gcaaggtttc agtaacatca catgctggaa ccacagcgcc gtcagttcca
     5581 gtgccagagc agtaaacgcc atcaacagcc acgcgccccg gccttgtgaa cattggttca
     5641 aaaatcgcaa cataatcatt tccctgcaat atgcatagag tgcgcagttt aaaccaattc
     5701 attcgctgcg ccaccaggag gcagcaaaaa taggacgcaa taggacaatt atcgggcgaa
     5761 aaggcaaacc gggcatttat atgcccggta agttgttatc aaagcgttgc tatccagccc
     5821 atttgcataa accattcggt tacaggggca agcgtaaact cgacgcagag caagccgacg
     5881 agtgtcagta cgagggtgta aggcagggcc atccacacca tgcggccata agagaggcga
     5941 atcaatggcg cgagtgcaga ggtcagcagg aacaggaacg cagcctgacc gttcggcgta
     6001 gcgacagagg gcagattggt accggtatta atggcgaccg ccagcagctc gtattgcttc
     6061 aacgtgatag cgccactttc cattgccgct ttcgcttcgt tgatataaat cgtccccacg
     6121 aagacgttat ccgaaatgga tgacagcaga ccgttgaaaa tatagaacag cgacagctga
     6181 gcatgttcag atgcctgcaa cacaaactga ataatcggcg aaaacagttg ttggtcgata
     6241 atcaccgcga ctaccgagaa aaacaccgtc aacagtgcgg tgaatggcag agattcggtg
     6301 aaggctttac cgatagaatg ttcatcggtg acaccggtca atgatgttgc cagaataatg
     6361 actgacaaac caatcaagcc aacttccgcc agatgcaacg ccagcgcagt caccagccag
     6421 acgccaataa tcgcctggac aatcagacgg attttatcct gacgggtacg ctggtggcgg
     6481 ctttgatcgt caaactgttg caacacttcg cggactttct ccggcagcgt ttcaccgtag
     6541 ccaaaccaac gcagcttctc taccagcagg caggttaaca ggccacaaat cagaaccgga
     6601 acggtcaccg gcgacatgcg caggaagaaa tcgccaaaat gccagccagc cgctttagcg
     6661 atgatcaggt tctgtggttc gcccaccatg gtcattacgc cgcctaatgc ggtgccgaca
     6721 ccggcatgca tcatcaggct acgcagaaag ccacggaact gttccagaac cacgttgtaa
     6781 tgcttgtcga tatgactatc gtcttgcagg tcggtgtctt cggtacggga agaggctacg
     6841 cgatgataaa taccataaaa accgactgca acgctgatca ccaccgccac gacggttaag
     6901 gcatcgagga acgcggagag gaacgctgcc gccacgcaaa aagagagcga cagcagcatt
     6961 ttggagcgaa tgcttaacag caaacgggta aatatgaaca gcaacagctg tttcataaaa
     7021 tagatacccg ccaccataaa catcaataac agcaagactt caagatttgc cgccacctct
     7081 tcacggacgt gttccgcgct ggtcatgccg atgaataccg cttcgatagc caacagacca
     7141 ccggggagta gcgggtagca tttcagggcc atcgccagag tgaaaataaa ttccgctacc
     7201 agcaaccagc ccgcgacgaa agggctgatg aggaaaatta acgggtttac gattaagaaa
     7261 atgatgaggg cgagtttgta ccagtcgggg gactggccca aaaaattgcg ccatagcgcg
     7321 cggccccagg agatctccat gatggtttcc cttaccttac aaataatcaa tgatgttttt
     7381 atgtttaaac gcaaagctta acggtcaggc aggagtgagg caagtcttga tagtcaaggg
     7441 gaaagagatg cggaaaatga agccttgatc cctttttctt ctttttgtct gctatcagcg
     7501 tagttagccc tctggtatga tgagtccaac tttgttttgc tgtgttatgg aaatctcact
     7561 atggtcatta aggcgcaaag cccggcgggt ttcgcggaag agtacattat tgaaagtatc
     7621 tggaataacc gcttccctcc cgggactatt ttgcccgcag aacgtgaact ttcagaatta
     7681 attggcgtaa cgcgtactac gttacgtgaa gtgttacagc gtctggcacg agatggctgg
     7741 ttgaccattc aacatggcaa gccgacgaag gtgaataatt tctgggaaac ttccggttta
     7801 aatatccttg aaacactggc gcgactggat cacgaaagtg tgccgcagct tattgataat
     7861 ttgctgtcgg tgcgtaccaa tatttccact atttttattc gcaccgcgtt tcgtcagcat
     7921 cccgataaag cgcaggaagt gctggctacc gctaatgaag tggccgatca cgccgatgcc
     7981 tttgccgagc tggattacaa catattccgc ggcctggcgt ttgcttccgg caacccgatt
     8041 tacggtctga tccttaacgg gatgaaaggg ctgtatacgc gcattggtcg tcactatttc
     8101 gccaatccgg aagcgcgcag tctggcgctg ggcttctacc acaaactgtc ggcgttgtgc
     8161 agtgaaggcg cgcacgatca ggtgtacgaa acagtgcgtc gctatgggca tgagagtggc
     8221 gagatttggc accggatgca gaaaaatctg ccgggtgatt tagccattca ggggcgataa
     8281 tcccttccgt ttaaagagca accccctcaa acgaggggtt ttttgttgtt tttacagatt
     8341 tcccattctt ggcgggcaac gttccagcaa ctcgatgctg ccgtcttcgt tttgctgttc
     8401 gagcatcaca tcaaatcccc acaggcgatg cacatgcttc aggacttctt tgcgcccccg
     8461 atccagcggt gcgcgattat gtggaatata acgcagcgtc agcgaacggt cgccgcgcaa
     8521 atccacgttc cagatctgaa tattcggctc cagattactt aagttatatt gcgacgataa
     8581 ccggttacgg atctcccgat aaccttcttc attatgaata gcggaaatct ccagataatt
     8641 atgccgatca tcatccagca cggtgaagaa gcggaaatca cgcatcactt tcggtgacag
     8701 aaactggctg ataaaactct catctttgaa atcacgcatc gcgaaatgca gcgtttccag
     8761 ccagtcggaa ccggcgatat ccgggaacca gtatttgtct tcttccgttg gcgactgaca
     8821 aatccgttta atatcctgga acatggcgaa cccgagggca tacgggttga tgccgctgta
     8881 ccacgggctg ttataggggg gctggaagac cacattggtg tggctgtgca aaaactccag
     8941 cataaaacgt tccgttactt tcccttcatc atacagatgg ttaaggatgg tatagtgcca
     9001 gaaggtcgca cagccttcgt tcatcacctg agtctgtttt tgcggataaa aatactggct
     9061 caccttacgc acaatacgca ggatttcacg ctgccatgat tccagcagtg gcgcattttt
     9121 ctccataaaa tagagcaggt tttcttgtgg ttcggacgga tagcggcgcg cttcagcaac
     9181 cgttttctct tcctcgcgct tcggcagggt acgccagagc atattgacct gactttgtag
     9241 atactcttcg cgacttttct gccgggcttt ctcttcttgc agcgagattt tttgcgggcg
     9301 tttgtaccgg tccacgccgt agttcatcag cgcgtggcac gagtccagaa gccgttctac
     9361 ttcatcaacg ccataacgtt cttcgcactc ggtaatatat ttacgggcaa aaatcagata
     9421 atcgacaatc gaactggcgt cggtccaact gcggaataag caattgtttt taaagaaaga
     9481 gttatgtcca tagcaggcgt gtgccatcac cagcgcctgc atagtaatgg tgttctcttc
     9541 catcaagtaa gcgatacacg ggttagagtt aatgacgatt tcataggcca gcccttgctg
     9601 accgtgctta tacagccgtt cagtctcgat aaacttttta ccgaatgacc agtgcggata
     9661 gttaattggc atgccgacgc tggaataggc atccatcatc tgttctgagg ttatcacttc
     9721 aatctggtgc gggtaggtat ccagccggta gagtttcgcc acccggtcta tctctgccag
     9781 ataaacatcc agcaggtcga acgtccagtc gggtccatcg ctcaaacgtg tggtgtcctt
     9841 attcatagaa tcgatcgtcg ccatacacgc acctcattgt tgtcggcgct ctctgtgtgg
     9901 agcacctcat ttcaagcata gaacacctgt taaaaaccgc gtcgccggag aatttttttc
     9961 tttgcgattt cttattatca gagtgccact aatccgcttc tgaacggaat tttatgctgg
    10021 ataaaaaggg cgttcagcag gagatactaa agacgccata ttgccgcaga gtcagggaga
    10081 tgtgagtcag ctcaccataa aaaagccgta tgttgaataa tattttcaac tgagttatca
    10141 agatgtaatt agattattat tcttttactg tatctaccgt tatcggagtg gctatgcgag
    10201 ttgtcatact gggaagtggt gtggtaggcg ttgccagcgc ctggtactta aatcaggcag
    10261 gacatgaggt caccgtcatt gatcgggagc cgggggccgc cctggaaacc agtgccgcga
    10321 atgccgggca aatctccccc ggatatgctg caccgtgggc agcaccaggt gtgcctttaa
    10381 aagcgattaa atggatgttc cagcgccatg cgccgctggc ggttcgtctc gacggtacgc
    10441 agtttcagtt gaaatggatg tggcaaatgt tacgtaactg cgacaccagc cactacatgg
    10501 aaaacaaagg gcggatggtg cgtctggcgg aatacagccg tgattgcctg aaagcattgc
    10561 gcgccgaaac caatattcag tatgaagggc gtcagggtgg gacgctgcaa ctgttccgta
    10621 ccgaacaaca gtatgaaaat gcgacccgcg atatcgccgt gctggaagat gccggcgtac
    10681 cgtatcagct gctggaatcc agccgcctgg cggaagtgga tcccgcgctg gcagaagtag
    10741 cgcacaaact gacgggcggc ctgcagttac ccaatgatga aaccggagac tgccagctgt
    10801 ttacccagaa tctggcgcgg atggcagagc aggcgggggt taaattccgc tttaatacgc
    10861 ccgttgacca actgctttgc gacggcgagc aaatctacgg cgtgaagttt ggcgatgaag
    10921 tgattaaggc cgatgcgtat gtgatggcgt ttggttctta ctcgacggcg atgctcaaag
    10981 gcattgttga tattccggtg tatccgctga aaggctactc gctgactatt ccaattgcgc
    11041 aggaagatgg tgcgccagta tccaccattc ttgatgaaac ctacaaaatc gccattaccc
    11101 gtttcgataa ccgcattcgt gttggcggaa tggcggaaat tgttggtttt aacaccgagc
    11161 tgttgcaacc gcgtcgtgaa acgctggaaa tggtggttcg cgatctctat ccacgcggcg
    11221 gtcatgtcga gcaggcgact ttctggacgg gtttgcgccc gatgacgcca gacggtacgc
    11281 cggttgtcgg gagtacacgc tttaaaaatc tgtggctgaa taccggtcac ggcacgctcg
    11341 gctggacgat ggcttgcggt tccggtcagt tgttaagcga tctgctctct ggtcgcacgc
    11401 cagcgatccc atatgaggat ctaagcgtag cgcgctacag ccgtggattt acgccatcac
    11461 gtccgggcca tttacatggc gcacacagct aaggaaacga gatgacccgt ccgatacagg
    11521 ccagcctcga tctgcaggca ttaaaacaga atctgtccat tgtccgccag gccgcgccgc
    11581 acgcgcgcgt ctggtcggtg gtaaaagcga acgcttacgg gcatggtatt gagcgtatct
    11641 ggagcgcact cggggccacc gatggctttg cattacttaa cctggaagag gcaataacgt
    11701 tacgtgagcg cggctggaag gggccgatcc tgatgctgga aggatttttc catgctcagg
    11761 atctggagat ttatgaccag caccgcctga ccacctgcgt acacagcaac tggcagctca
    11821 aagcactgca aaatgcgcgg ctaaaagcac cgttggatat ttatcttaaa gtgaacagtg
    11881 ggatgaatcg gttaggcttc cagtccgatc gcgtgcttac cgtctggcag cagttgcggg
    11941 caatggcgaa tgttggcgaa atgaccctga tgtcgcattt tgctgaagcg gaacatcctg
    12001 atggaatttc cggcgcgatg gcgcgtattg agcaggcggc ggaggggctg gagtgtcggc
    12061 gttcgttgtc caattcggcg gcgactctgt ggcacccgga agcgcatttt gactgggttc
    12121 ggcctggcat tattttgtat ggcgcttcgc cgtccggtca gtggcgtgat atcgccaata
    12181 ccggattacg tccggtgatg acgctaagca gtgagattat tggtgtccag acgctaaaag
    12241 cgggcgagcg tgtgggctac ggcggtcgct atactgcgcg cgatgaacag cgaatcggca
    12301 ttgtcgccgc aggatacgcc gacggttatc cgcgccatgc gcctaccggt actcctgttt
    12361 tagtggacgg cgtgcgcacc atgacggtgg ggaccgtctc gatggatatg ctggcggttg
    12421 atttaacgcc ttgcccgcag gcggggattg gtacgccggt tgagctgtgg ggcaaggaga
    12481 tcaaaattga tgatgtcgcc gccgctgccg gaacggtggg ctatgagttg atgtgcgcgc
    12541 tggcgttacg cgtcccggtt gtgacagtgt aacttgttgt aagccggatc ggaggcagcg
    12601 tcttctggtt gcgaaaaaat catccatccg gctggtcagc aactgtagtt gttaatgtga
    12661 cagagccatt gcccatgata gtgtccatta aaagaatgga cactatttcc ccggaacctg
    12721 aactcgccgc acaggcgttc tacataaaac gcttacgctt cattgttgac tcggagtcga
    12781 cttcagataa atcacgcttc acccttgatg cagattcggg ttcatgttca gattgttgat
    12841 taacaggctg cgagtcctgt tgtctttcca gactcaacag gcccaactgt tgatccagga
    12901 gctccaggtc ctcctgtacc ttcacgcgct ccaggagttc cgggtcatcc aggaactcca
    12961 gatccataag gtcctccagc tcctcgagtc cttccaggtc aacctagcct tccttgtgca
    13021 aaaagtccaa aaggtccttc aggccctgct gttttttcag gccctcgacg gctttgagca
    13081 gcgtcggtaa attgtctttg tcccacgggt taccattaca gtcaaacacc ttcaggctct
    13141 ggggctgcgg cacctgtacc gttttaaact tgttttctcc acagtagtaa attattagtt
    13201 tgggcggcag ccgtggtgaa gagatcaaat cgttactaga acagtcaaat actttcaggc
    13261 gagtcaagtt ctccagcatc tcatcaggta ttgagttaat attgttgccg gcacaggata
    13321 gtttatacag gtactggggc atcgacggcg gtaaaaattg caagaagttt tcagaacagt
    13381 taatcactct caggttagtc gacagctcag gcagccagat aatactgttt tcatagcact
    13441 tcagtaccat cagttttgag ggctgcacct tcggtaacaa gatcaagacg ttatctgtac
    13501 agtcaagcac cagcagccac tttggcatca ccttcggtaa cgcggtcaac acgtttttac
    13561 tgcagtaaaa ttcaatcaga tgtggaggca aattctcagg gagtgaagtc aatcccaggt
    13621 tgctaagatt gagcatactc cgtcttgtaa tgagacattc cttcattctg cgcaatgctt
    13681 ctgcccggtt ttcaatccgg cttgctggca ggttttcttc caggatattt tccttgattt
    13741 cttccagact ttctttcagg tcttcttggg gaatttctga ctgtttgttt tgctggtttt
    13801 cttccaggat ttcgtacagg tttttttgca ggttttcttg caaaccatcc tgctgtatag
    13861 cccattcgca ccatttggcg accatcatga actcgttatg ttgctgattc agacgagtcc
    13921 ccaacgtgat gatggtattg ttgtccacct gtatgttcac tttgcccgcg atactgaggt
    13981 caatgatctg ccagggggca tcaccccagc tgaatttttg cgggctgaga tacttcatgt
    14041 tctgcaaaat ttgattcttg agaagagccg ccctggcggg cgtcgtacgc ctgggttaac
    14101 ggaagatggg ggaatggcgg gggcgaatgg gcagatgtct ttaaagctag ccataatgct
    14161 acgtttcctt gaaataaata tcctctggca gcgctggaga cgtgaatgtg gtcaccgcaa
    14221 aggagccaca ggacgtcatt tgcgctgata tcactactca tcgttgactt tttccctggt
    14281 cagaaatgcc gtgtcgggtc tggtacatgc tgtattacgg cctaggtagc tctgacgtat
    14341 taaagaaaat acgagagtga acgacccgga aataatcgtt attaagcaaa ggaataacta
    14401 taagaaatcg ctcatgcatc cggtgctaca ggcggatgaa acgcctattg tgtgggctat
    14461 gtcagtgcgg caggttgcgt gggggaatat gttcaggcga tgtcaggcag gagcccctga
    14521 tggttgaccg gtaatgcggg gctggttttt attacctgtt acagtcggaa aaagccctta
    14581 cgctgacttt cttgagccgg tgttttgagt tcattagcca gagcctgcaa tggtttgtca
    14641 tccataattt aaccttcttc tgatgttggg atgagcatat caaaatcagg aaatgacaag
    14701 tttattacag gctcaggctg cgttcagtaa agcatcgcat ccggcgatag atgctgcatt
    14761 aaatttaaga ttcagcttcc tcttcagcca cccgaacgcc aatcttcaac acttcattat
    14821 cttctttctc ggccaccgtc cagatcatcc cggcaaactc tacctggtca ccgacaaccg
    14881 gtgccgcgcc tagcaactgc tggacaattt cacccagcgt ttgctgttta tcacgatatt
    14941 ctcgcccgtc ttccaggcca tatatcagcg caacatcagc atatttggcg ctggcttcga
    15001 gaatgaagtc accaaagaag cgttgatcca gcgcgaccgg tggcgactgg ctgaacagtt
    15061 taccgagcgc cgggagatcg cgttcccgac caattacaca caatacatcg ccttcgcgca
    15121 gtcgggtgct gccggtagga tgaagcaact ggttatcacg aaacagggct gcaatacgcg
    15181 tctcttttgg catatgcaaa tcgcgcagtg ccgcacctac acaccattta tcagcactca
    15241 attgataaac aaactgctcc cacggatttt ccggatgaat atccaggcca acgcgtgaca
    15301 ccggacgtcc gacgggcgga accaccactt tggctttttt cgccgcccac gagagtgatg
    15361 ttccctgcaa gagcagtgaa accagaacca caaagaaggc gacattaaag aacagtcgcg
    15421 cattctccag ccccgccatc atcgggaaca cggctaggat gattggcacc gcgccgcgta
    15481 atcccaccca gctgataaac acgcgctcac gcagattgaa accgcggaaa gggagcaatc
    15541 cagcaaatac cgaaagggga cgggcgaaga atatcatcca tgccgacaaa atgagcgccg
    15601 gaatggcaat aggcagcaga tcgcttgggt taaccaatag ccccagcacc aggaacatgg
    15661 cgatttgcgc cagccaggcg aggccgtcga aattttgcag gatgccgtag cggttgcgaa
    15721 tcggccgatt acccagcaga aaaccgcaca gataaacagc cagaataccg ctgccttcca
    15781 gcgccgtggt taaagcaaaa attaaaatac cgccgcttaa tgccagcaat ggatataatc
    15841 cggcaggcag ggcgatgcga ttaatcattt gcagcagtaa ataaccaccg ccaagaccga
    15901 tgacaatccc gaggccaaat tgctgcagaa tatcgaccac gaacatccaa ctgacactgc
    15961 tttcatgctg ctggatcatt gcaatcaggg taatcgtcag aaagaccgcc attggatcat
    16021 tactgccgga ttcaatttcc agcgtcgagc caacacgttc gttaagcccc ttaccaccca
    16081 gcagagaaaa gaccgctgca gcatcggtgg agccgacgat agcgccgatt aataagcctt
    16141 caatcaaatc aagattaaac agccacgccg ccatcatgcc ggttaaacca gaggtgataa
    16201 gtacgcccag cgtcgccagc gacagcgccg gtcctaacgc cacacgaaag gaactggcct
    16261 gagtgcgcat cccgccgtcg agcaaaataa tcgccagcgc caggttactt accatgtagg
    16321 cgaaggggta attatcaaac gggataccgc cgacgccatc gactcccgcc agcatgccga
    16381 tcgccagaaa aataaccaga ataggaatgc caagacggga agaaaatgaa ctaagtaata
    16441 tactgctggt gacaaggatg gatcctaaga tgaaaaggct aattattgtt gtggcatcca
    16501 acggtcggtt actcctgatt acgctgtctc ttatataaac cctaccatat tagcggcaga
    16561 gacagcgttt tacttagccc tgaagcgtga tttttttaca ttttaagaac aggatgaccg
    16621 ctaatcgtca actgagcgcc ttcccgggtg ttattcagaa tggcatgtgc gcccaacggc
    16681 agcgtaaccg tgcgttgttc atgaccaaaa tcgaggccgg taatgagtgg aatcgacagg
    16741 cgggaacgca aaaacgcgta cactgactcc aggttgtaac ctgcgtcata atcattgggc
    16801 gtgctgccgc taaagctacc gagaataatc gccttctgac gtggcaaaat tcccgcatga
    16861 tagagctgca acagcatacg ttcgacgcgg aaagggtgca cgttaatatc ttccagcacc
    16921 agaataccgt tctcaatttt tggcatccac ggtgtaccaa tcagtgaaat cagcatcgca
    16981 agattgcctc cccacaacgt gccttcggtc tgacatgtcg ggccttcccc ttgccattca
    17041 atggtgaagg tttcattgcg taacgccagc cagaaatggt gctcggtaaa ggcgttcagt
    17101 tcatccgcac caaagtttgc taccagcatg gggccgctaa aggtgatgac attgccttgc
    17161 gccagaagac cgcactgaat ggcggtaaaa tcgctatgtc cgcaaatgag taacgggtca
    17221 tgttgttggc gagccaccag tgcctgccag tcaatatctg ccagtaaacg actggcaccg
    17281 taaccgccgc gtacagccag cacgatggtg ttgggggtag tcagtctggc aagggaattg
    17341 agatcttcca gacgctccgt ttccgtaccc gcaaaacgct cacaacgacg ggcaataacc
    17401 tcgacgttat tgacttgatg ccccgcgtcg gtaaggcgct ggataccacg cagcgcggcg
    17461 tgctgtttaa tgctgtaacc cgatggggca attaagtgaa acagagacat ggcaattcct
    17521 tgctgacaac agaaacgaaa tgtatatcat gccgcttagg tgtgccgttg tcacctcaac
    17581 ggcgattcca ggctataagg atagaagaag tgaaattgag atggtttgcc tttttgattg
    17641 tgttattagc gggttgttca tcaaagcatg actatacgaa cccgccgtgg aacgcgaaag
    17701 ttccggtgca acgtgcgatg cagtggatgc caataagcca gaaagccggt gcagcctggg
    17761 gcgtcgatcc acaattgatc acggcgatta tcgctatcga atcgggtggt aatccgaacg
    17821 cggtgagtaa atcgaatgcc attggtttga tgcagttaaa agcttcaacc tccggacgtg
    17881 atgtttatcg ccgtatgggc tggagtggtg agccgacgac cagcgagctg aaaaatccag
    17941 agcgtaatat ttcaatgggg gcggcttacc tgaatattct ggaaaccggc ccgctggcag
    18001 gcattgaaga tccgaaggta ctgcaatatg cgctggtggt gtcatacgct aacggggcag
    18061 gtgcgctgct acggactttc tcgtcagatc ggaaaaaggc gatcagcaaa atcaacgatt
    18121 tggatgctga cgagttcctc gatcacgtag cgcgaaatca tcctgcgccg caggctccgc
    18181 gctatatcta caaacttgag caggcactgg acgcgatgta aatcagtcgc gcactttgtc
    18241 cgctttttcc cgggcttctc gctcgagaga gaaaataatc cgctgtaatt gccgctccac
    18301 cgtcgggctg acgttaagaa aacggaagct cagacgggga gtggtgatgg tttcattctt
    18361 gccatcaatc actttgcgct cgctggtgga gattaactgg gcgtcaaagt gaaaaacacc
    18421 ccattgcccc atgttgactt caatttgagc gaagcgcata ccttcttgta attcggcagg
    18481 ctttgctgtt tccagtaatg cgcccatgcc gcctaacgac aaatcataca agcggaaacg
    18541 taacgtactg ttatccgcca gtttggtctg gcaaaaataa ggcggatgga gtggggcgga
    18601 gatgcggaaa tatcggcgtc gttgtacaaa ccataaggtg ggaggcggta cggtaataaa
    18661 tgccggaagc tgcaagtatt cactctgctg tagttgttca acagtaaact cgactttcgc
    18721 accctgagtt tcggcggtaa tggtaatgtg ctgtgccttt agtacggcga tgttgtcttc
    18781 ggcttgactg ccgaaatcca gcaccagttt atccggggtt attgccagta ttttgctgat
    18841 cagctgcccg ccattccaac tgagacgcaa aggaattgcg gctttgtgca aatcgcgtaa
    18901 cacgcccagg acggctaacg gattttgttt caggaactgc tcatggtaat gactcacgcg
    18961 gaaaaactcc tgactgacaa actgtctttg gtttatcggt catagttaac aaaacttaat
    19021 acaaatgcgt gaatattttt tacatgttgt tcttaaatca gccgacaaac gcccagcatc
    19081 gatcccgccc ttgcctatac ttagagcgtt gacgtaagca tttcttgcga ccgattcatc
    19141 gaaacgaggg cctgaacatg ggaattattg cctggattat ttttggcctg atagccggca
    19201 ttatcggcaa gctaatcatg ccggggcgtg atggtggtgg atttttcctg acctgtattc
    19261 tcgggatagt cggtgcggtg gtcggcggct ggctggcgac catgtttggc attgacggct
    19321 ccatcagcgg ttttaatttg cacagcttcc tggtggcggt ggtgggcgct atcctggtgc
    19381 tgggcgtatt ccgcctcctg cgaagagaat aagattttca taaggcggat agcgatacag
    19441 atgccgctat ccgctttcac atcagaacgt atactcgaca cctgcccaga aattgcgacc
    19501 ttcttcaata aacccttcac tgtaggcata ttgggtatca aacaggttat tgaccgacgc
    19561 attgacgctg aagccatgac ctaaggtgta atcggctcga atgtgggtca ccgcaaaacc
    19621 ggcggctttt tgtgaaccgt cactgttgct gtagctggag gaacgcgcct cttccgacag
    19681 cgttacgctt aacggctccc atggtttgag tgtcatccat gcggtcattg tctgcgttgg
    19741 aagatcggtt atcttgccga tgtctttgcg tttggcgtca gcgtggatca gagcgtagct
    19801 caatcctaca tccagtatat tgctgatttt ccccttaata ccggcatcca gaccgctgta
    19861 atccaccgtg ccgctgttct gattttgaat ggtatcggca ttgatattgt gcgagaggat
    19921 ggcatcactc acccggttat agtaaacgct gacctcaaat ccccagtcgc gcgtgaaggc
    19981 accattccag gttaaatcca ccccgcgagc gcgttccggc ttgagctgcg ggttaacaat
    20041 cgctatctgg ttatacgcag gtttggatgt ggtatagcgt tctttcagcg tcggaaagcg
    20101 tgtgcggtca tagtacgaaa gcgccagagt gtcttcattg gcaaagtggt atttccccat
    20161 cacctgccag ttaaaagctg actgattgtt gtcgtcataa tgggtgatgc tgccatcttt
    20221 ctcgtgtttt ttcgcttcta cgctatcgcg ccagtcatag ctgattcccg ccacgacatc
    20281 gacattatca gcagcagccc attgatattc actggcgagc gaccaggtac gatcttcata
    20341 gcgatcgtaa gcggcgtgcg gcgcaccttt ttcccggtgt acgtcatctt tccagttaac
    20401 ggcaaacgac agcagatcgt tttcacgcac atcggctgcc agttgtaatc cggcaccgtc
    20461 gctgtaatcg gaataatggc tgtagctgcc ttttttattt ttcaaatcag ccagcgagtt
    20521 gtacatcatc agcgtatttt caaaggtgtc gcgatacagc cgacttttca gggtaaaacg
    20581 gtcgtttagt tgggtcgctc cctgataata aaaactttct ttgtcatact ctggccactg
    20641 ccagtagcgt gatttttgac cacaatttcc gctgtatggc gggttatctt tttcaccatc
    20701 ctgcttaatg taagtcagcg tgtattcatc gttttcgcgc ggtgtaaaac cgagcttcac
    20761 aatgccgcgt ttatcatcag ccgatgaatt aatcatcttg ccgtgtttgc ctgcaatatc
    20821 attatttaca ccatgcggca ggccgagaaa atcctgcttt agctggctac cgctgacttg
    20881 caaataccca agatcgctgc tggcggcaaa tgaagcatgc atatcgtagg cattgtcctg
    20941 actacggctc catccctggc gatatcccag acttgcttct tcaggggggt gatattaatg
    21001 gctccgccca tctgattagg cccatgaagc agcgacgaat accctttgga aacttcgact
    21061 gcccccagat tgttggtcag aatccgcgcc agatcgaggt tgccgtcata ggggacataa
    21121 atgggcacac cgtcgaaata gaccggcacc tgccgactat caaagccacg aacttttacc
    21181 tgttcttcgt tgcggctgcc tgacttttgc agcaccacgc cggggacgac acttaatgcc
    21241 tgagcgacat tctgtttatc caaggccttc atggtgggtt gatcgagaac ggtggtcgtc
    21301 gtcgatgata ccggactgga ccatacggtc agcgtgtcgc tctcctgagc cgctacggcc
    21361 tgctgggtaa aggcaagtga taatgctgtg cagagataac gttttttgag tctcatagaa
    21421 taaattccgt ttaacaaaga attaaataga ggctgtgggt tgcagactga ctttaatgtc
    21481 agctggtgcc gcgtaataag gcgcggaggt aataaaaagg cgaatgccgc agtcgaggta
    21541 atttttgagt gtcgtcagat taattccgcc ggtcagagcc agcgtgcagt gaggagccag
    21601 cgacggcgct atttgcgcaa tttcagttgc ctgttgcgga ctaaatttgt cgagctgaag
    21661 tacgtctggt tgtgcgcgta acgcggcgat agcctctttc ggcgtgtcgg cttcaacaac
    21721 aattttcttc tcgggtgcgt ggcgacgtaa ctgagcgatt gcgcctgacc agccctgatt
    21781 gtcatgaaga aaatggcggt ggttggcaaa caataatatg gtttccgcac atccggcacg
    21841 atgaattagt cctccggcag ccagaattgc ctgcgaagcc agtaagcggg tgcccggaat
    21901 tgcttttcgg gtgcaggcga tattaccgtc aggataacgt tcacgaagca atgccagcat
    21961 ttgagcgaga taatcagaaa caccgcaact ccactccagc acattctgga ctgccttcca
    22021 tccctgatga agtgctgcgg cattaccctg cgcacggatt agccgttgac ccgcgttcgc
    22081 ttgtgaaccg tcgctgaccg cgtcatcaat agttaatccc agcgtagtta acatcttaca
    22141 cgcgacagaa acgccgctga cgcagccgcc ctgacggtga aaaaacgcta tatagccagg
    22201 ctggtgtcca atatttaatg ctcgcgtggt caggtcacct ccctggatat cttccagcag
    22261 taacgcatcg atttgtgcct gagaaaggaa aatcatcaca gtgcctcctg cggtacgcta
    22321 tcccaccaga tgagcgccca gtcgcggctg gcaggggcga gggcacgagc atcttgttgt
    22381 tgataccagt gatataaccg ctcacgctca tcgtcgttaa gcgcgccaag actccagctg
    22441 acgttctgct caaaacgttc ccaggtgctg ttatcctgtt ggcaattttg tccacggata
    22501 aaatcaacgt gggcataaat tcccatctga tacaggacgt tgagcgcaaa gatatagttg
    22561 ggtaattcga tcacttcccg accagccgcc cgctgaatgg ctggagagac gaacgaggtg
    22621 ctgaccagat gagttgtata gacgcgaagc cgtgcctgat tattcagttt gctcattgca
    22681 tggcgcatat cagccaccag agtggagcgg gaagccacgg caatatcgca gcgtggcagg
    22741 tcactccagt cctcttccca ggcgcgctgg atccagtgga cattgtcagc cttcagtgcg
    22801 gcggcgcggc gggcggcaac gttcagcatc ccctgactgt aatcaacgcc ataaaccgta
    22861 gtgagtttgt cagccagcgc cagcgatacg gtgcctggtc cgcagcccat atcgaataag
    22921 gtctgagcgc cttgcaggtc aattttagcg atcagttgtt gcaggtagct gtccgttggc
    22981 gaggcgcaat tttccgccat tttctccgcc cgttgatccc agtgatcggg cgttttttct
    23041 gtgcggtgag ccagtttgag ttgctgcaga taaaggtctg caaaatcaat atcatcaatg
    23101 agcatgattg ttccttagcg gctaattgca gagaggtgat gatgaatttg gtcggcagaa
    23161 acccgataca gggccgccag tttgttcgtt gttaactgtt cggtcggtaa gccttgtgtt
    23221 actcgtccat ctggctctac ttggataatg ctgtcggcaa tggcgttggc atggagcgga
    23281 tggtgagtcg acattaacat tgtcatgccg ttatttttga gctgcgccag cgtgtccaga
    23341 agttggatct gatgaccgaa atcgaggctg gaggcgggct catccagtaa caacaagcgc
    23401 ggttgttgga cgagtgcacg ggcgattaaa gccagctgac gttcaccgcc gctaagcgta
    23461 ttccagcgtc gtgtcgccag atgcaaaatg cccagctttt ccagttgctc cgtggctttc
    23521 aggcgttctt gcttaccggg aacggaaaaa gcgcctatgg tgggggccag gcccatcaac
    23581 accatatcca gcacactgaa agcaaaaata ccgtcgtgcg cctgcggcac ccaggcaata
    23641 gcctgtgcgc gctggcgatg gctgagtgtc tgcacagaga ggctatccag taggatctcg
    23701 ccttttaata acggtataac gccaagaatg gagcgcatga gtgtggtttt gccgcaacca
    23761 ttcgcgccaa gcagacaaca aattgttcct tgcgacaggg tgaaggacac gtcgcgtagc
    23821 actggatggc gggagtaacc cagagacgca tgacgaacgg caagcaaagt cactggcgac
    23881 ctccgcgtaa caacaaaagc aggaaaaacg gtgcgccaac aaaagcggtc agaatgccaa
    23941 gtgggatttc tgttgttcca atgctgcgcg ccagcgtatc ggtaagcagg agcagaatag
    24001 caccagtaca cattgccata ggcaacagct gctgatggtt atgaccggtc aacaagcggg
    24061 caatgtgcgg aacgaccaga ccgatccaac caattatgcc cgctattgct accgtgctgg
    24121 cggtgattag tgtggcgcag acaatcaatc caaagcgcag acgcgtcacg ttcagtccca
    24181 gcgaacgggc ttcgtcgtcg gaaagcgtga gtaaattcat gcgccagcgg agaaaaaaca
    24241 ggggcaagga gcctatgaga atgatcggtg cagcatagca gagatcgcgt aaggtcacgg
    24301 tggacagtcc ccccagcaac cagaaggtaa ttgacggtaa ctgggtgtaa ggatcggcca
    24361 gcgttttaat tagcgatatc cccgctccgc acaacgttcc taacgcaatg cctaccagca
    24421 ccagggttaa gatcggatcg tggcgggtca cccgacgcgt aatcaaccag acgcccgcga
    24481 ccaccatcag cccgccgcaa aatgcgtaca gctggatgta aacaatgggt aatccccaca
    24541 ggattgccgc gcaggcacca agtccggcac cggcggagac gcctaaaata tccggtgaaa
    24601 ctaagggatt acgcaacatt ccctgatagg tggttccggc tcctgccagc gctgcgccca
    24661 gtaacagcgc agcaagaata cgcggcacgc ggatttgcca gaagacaatt tgttcctgca
    24721 cagactcatt ttcttgacga agaattagcg ccagcagttg ctgcatatcg agatgataag
    24781 ccccacttac ggcggcaaaa gtgatactga tagctgccag cagcaaaata catccgttta
    24841 cgattctcat tagctcgcca ccaatttttg atagtcggca tcactgagtg aacaatgcca
    24901 gaacagttgg gcgtaatgct gcatgtcact tttaaactgg cgattgatgg cgggatccag
    24961 ccaggcgtga agtctgcgta atcccagaag acggttgatt cccggcgggg catcgagcca
    25021 gccaaagggc aagccactta aaaataagat gcgttgttcc gcaacggctt tcacgccctg
    25081 ccagagagga tcacgacgaa taaaatctgc agtaacggcc tcctgaacca gaataatatc
    25141 cggctgccag cgcaggagat tttccatgga aacctgtgtc aggccgtggc gatcggctat
    25201 ttgcgcgacg ttgtgcaaac ctaataattc cgccgcctcg gtatgcaacg aaccctgtaa
    25261 gcccgtttcc agccctcggg gaccgcgcgc agcataaaag cggaggttag cggcgggtga
    25321 ggtggcgaat gcctgagctt caccaacgaa gtggctggcg agattggctt gttctgcggc
    25381 gcggtgctct tcgcctaacg ttttgccaag cgttgttaac tgttctgctg attgcgccag
    25441 ttttccgtta agcaataacc aggggatttg tgtctgttcg ctaacctgcc gtgcctggga
    25501 gatcaaggtt tcatccgtat tgccgcaatc aacgaccaaa tcgggatgta acgccataag
    25561 cccttccagc gatagtgtgc tggcgcgccc ggcgagtcgt cccagcctgg gtaactggcg
    25621 aatgtgctct ggcaatggaa ttaatgcctg acgggcaaaa tcaaaagagg aaaaaccgac
    25681 cattttttct ggcgcgacag ccagcaagag caaatcggcg ggcgcgcctg cgctgacgat
    25741 gcgtgtaatg ttttgggctt cgggaagaga ttgcgaagca aaacgattaa ctgtttgtga
    25801 aaaggacggt agagactgca ggagcagggt agatgcaagt gcctgcgcaa acgtccgtct
    25861 ggttatgggc atggagaaca tcctgaaaag taaaatcgat atatactttc atatatatcg
    25921 ataaaaatca tgttcaatat caaagtttag ctattgaaaa catgaagatg tgacgacttc
    25981 cgcaagcctg ttgaccctct ctatcattaa aaatgtattt atcaggtggc taatattatt
    26041 taaatcattt aaggctaaat catttaaata tgaatgacat tattgttttt tatcgtccac
    26101 ggttacgcga cgttgaagtt acttgtttta taatgagcga tttttgatat ctcgccgaga
    26161 aaattatcag cattctctcg tttcagtgtc gtgaatatac tgttaacgga ggtgaaaaga
    26221 atgctggggg ttatacaaaa cctatgttgt tgtcgtccgg attcaccgct tgctgatagt
    26281 aagcagcgta accagaagtt accgcaacaa gactttgagg attttaagaa cgacgttatt
    26341 aatgtcatta acaacaatga agatatttat tttcttagag agagtaattt actgaatgcg
    26401 tttattcgga cgtgcaaaaa aagagatgat aacagttacg gaaatatgat atttacgctt
    26461 tattttgata acgatatcta tgggaacaga attatcaaag catcgctgaa cggcggagag
    26521 ttgcagttat taagcacagg gtgtagcaat gataacattc tcc
//
LOCUS       DAEEUE010000052        26076 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.052, whole genome shotgun sequence.
ACCESSION   DAEEUE010000052 DAEEUE010000000
VERSION     DAEEUE010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26076
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.052"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="KLK84_002328"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="KLK84_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HBE2009628.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="KLK84_002329"
     CDS             complement(2138..2584)
                     /locus_tag="KLK84_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBE2009629.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="KLK84_002330"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="KLK84_002330"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HBE2009630.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="KLK84_002331"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="KLK84_002331"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBE2009631.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="KLK84_002332"
     CDS             complement(5347..6009)
                     /locus_tag="KLK84_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HBE2009632.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="KLK84_002333"
     CDS             6182..6595
                     /locus_tag="KLK84_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBE2009633.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="KLK84_002334"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="KLK84_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HBE2009634.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="KLK84_002335"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="KLK84_002335"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HBE2009635.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="KLK84_002336"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="KLK84_002336"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBE2009636.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="KLK84_002337"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="KLK84_002337"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HBE2009637.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="KLK84_002338"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="KLK84_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HBE2009638.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="KLK84_002339"
     tRNA            complement(9861..9948)
                     /locus_tag="KLK84_002339"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="KLK84_002340"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="KLK84_002340"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HBE2009639.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="KLK84_002341"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="KLK84_002341"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HBE2009640.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="KLK84_002342"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="KLK84_002342"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HBE2009641.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /locus_tag="KLK84_002343"
     CDS             14005..14592
                     /locus_tag="KLK84_002343"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HBE2009642.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="KLK84_002344"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="KLK84_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HBE2009643.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="KLK84_002345"
     CDS             14984..15841
                     /locus_tag="KLK84_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HBE2009644.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPM"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="KLK84_002346"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="KLK84_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HBE2009645.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVVTGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="KLK84_002347"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="KLK84_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBE2009646.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="KLK84_002348"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="KLK84_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HBE2009647.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="KLK84_002349"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="KLK84_002349"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HBE2009648.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22444)
                     /gene="etk"
                     /locus_tag="KLK84_002350"
     CDS             complement(20264..22444)
                     /gene="etk"
                     /locus_tag="KLK84_002350"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HBE2009649.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKLGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVSAVLARAMLR
                     RGVEAPEQLEEHGISVYSTIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSNAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGTILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(22464..22910)
                     /gene="etp"
                     /locus_tag="KLK84_002351"
                     /gene_synonym="yccY"
     CDS             complement(22464..22910)
                     /gene="etp"
                     /locus_tag="KLK84_002351"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HBE2009650.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(22898..24037)
                     /locus_tag="KLK84_002352"
     CDS             complement(22898..24037)
                     /locus_tag="KLK84_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HBE2009651.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24083..25243)
                     /locus_tag="KLK84_002353"
                     /pseudo
     CDS             complement(24083..25243)
                     /locus_tag="KLK84_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
     gene            complement(25322..26017)
                     /locus_tag="KLK84_002354"
     CDS             complement(25322..26017)
                     /locus_tag="KLK84_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE2009652.1"
                     /translation="MLNTYVAEGKAASAKLIRSTLVDVFREAIAEGHVATNPVTATRT
                     AKSEVRRSRLTANEYVAIYHAAEPLPIWLRLAMDLAVVTGQRVGDLCRMKWSDINDNH
                     LHIEQSKTGAKLAIPLTLTIDALNISLADTLQKCREASSSETIIASKHHDPLSPKTVS
                     KYFTKARNASGLSFDGDPPTFHELRSLSARLYRNQIGDKFAQRLLGHKSDSMAARYRD
                     SRGREWDKIEIDK"
BASE COUNT         6217 a         6647 c         6906 g         6306 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgcctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggccggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgatgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcaggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaagt ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagcag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccaggaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaaa
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgctact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacgcg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgatgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtc accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggttac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgccg cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc aatccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaacg aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg cgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgtgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gcctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga gtaagcgaca gcgatcagcg
    22321 tgaataacgc ggtcacgctg ataataaact tacggtgatc ccataactcg ccgaccagac
    22381 gaagcagatc gatctcattt tcctgagtgc tgcctggtgg cgtattcata tttttagttg
    22441 tcatcgtatt ttataaactc aaattaccgg ctgaggcgct tcgcccattc ctgactggcg
    22501 cgctccaaca taccgtagac atgttcaaat gcgtcctgac ttttacgata gggatccggg
    22561 atctctttct gttccagcca ttgcccaaac agcattgtct tcccgcggac ctcgggagca
    22621 attgcggtaa cctgagcaat atgttccgac tccattgcca gaatcagatc gtaatttcgt
    22681 gccatctcag cagtgagctt acgtccggca tgtccttcaa gagatacgcc atgattagcg
    22741 gcaacatctg ccgctgtcgc atctgcaggg tgttttacca gaccatgaac acctgccgat
    22801 ttgactttta cgcccggcag acgtttacgc agcaagcgct cgccaatggg cgaacggcaa
    22861 atgttgccgg tacaaaccac taggattgag ttaaatttta gttgggccat gtttgaatcc
    22921 accgtgaagt ctctgtcagg tcatgcaccc cggaaatcgt cggtaccagc agagaaatca
    22981 cacggttcca acgagccagc ggcgcggtag tgacataaac aatgtcgtaa ggttcaagct
    23041 ggaattccgt tccgagtatc attgccgatg cgtccttcgc attcagctga taaatattgg
    23101 cgattttgcc attctgcttg ttttgcgttg cacgaataac aaaaatcccc gtcgcatcag
    23161 cgacatcctg attcataccc tcagcgttac ccagcgcttc tgccagggtc ataccgctgc
    23221 ggtccatttt cagcgtgctc tgtttaccca cttcacccat cacgaagact ttcagatcat
    23281 cgttgcgcgg aataaataaa atatcgcccg ggtgcagcag tttgttctgg cgcaagtcgc
    23341 cacgctgcat cagcgcatag aggttcactt tggtctttac gccattctgg gtgagcacca
    23401 cgttgcgcca gtctgcatct gcggtcagac cgcccgcggc gttaatcgca tccatgatgg
    23461 tcagaggaat attggtaatc ggctgctgac ccgatttgct gacttcgccg gtgacgtacg
    23521 ctttttgcga tcggaacgcc gcgacgctga catcgacttg cgggctttca atgactgaat
    23581 cgagacgcgc tgtaatctcg ttgcgtacct gggtaagtgt tttcccggcc actttcaggc
    23641 gaccaatata ggggtagaaa atcgcaccat cggcgttaac ccagttgccg gtatcactgg
    23701 cgctacggta ttgccctgcg ggggtggtta gttccgggtg atcccagacc gtaaccatca
    23761 acacatcacc aatcccgata cgatattcat agtttgcgat ttgctgctcc agctccgggt
    23821 tagcctgcga cataatcggt ttggcgcgca gctgatcaat caatcctggc gtgacgggat
    23881 agatattgac cattttgtcc aggtcatact gattgtcagg tagttcaatg acatccttgt
    23941 tactggtcga taaattctga ccaggaacaa gggtacatgc tgtcaaagac agaacagcca
    24001 atgtcagtac cgaaaactta aaaatgttct ttttcattgg tttatattta tcagtcaatg
    24061 taatgttatg aattacgtca ggttattgtc caaccggtac ttctcgctct gccgtcattg
    24121 gatcgagctg gtatttgcgt gacagcattt gtccaccatc acgcgtcaat ggtgtccacg
    24181 aaaccaccgc gcggttgcgg ttaggtccaa tggtcatcaa gtcgaacgga atcgagatat
    24241 aaaaaccttt actaaagccg ccttcgccgt agtcatcttt agatacgtta ctgattgccg
    24301 cccatacccc taccgccacg ccgctgtcaa agcgtttggc gacgtcgatc gttgcgcctt
    24361 tatcttttgc cagatattgc ccaacgctaa gtttcatcag tacgccattg agcgtcggcg
    24421 ggttccagta agccgtcacg aaacctgtgg gcgtggaata atcggtgaag cgcatcatgt
    24481 tgtcccagtc acgctgcttc acgtagttaa cgtccacacc cagcgcccag ctggcatcta
    24541 gcgggcgata aagaagctcg gaaccgacac cggcatacat cgtttccaga taaccgccat
    24601 acacctggcc atagaaacca ttgcctaagt cggcaaagta attcgcttgc aagttgttga
    24661 gataaacgtc attgcgaacg taatcacgga tatgcgtgcg cacgcggggc agggtagagt
    24721 ccgcgggtaa cagtgaagac ttaaacttgt cgtagttgtt gtaaatattg gtgaaaatac
    24781 cgtcatcaag cagcaggtgg tcggtaaacc agtagcgggc gctggacatc agccccagct
    24841 ggaacatata gaaatcttcc gggccgccca gcgactgtga aagtgttggg ttgaaagagt
    24901 agctaaagcg atcttcacga atacggtagc cccgaccaaa ggcagaaaga tcttctgctt
    24961 caacacgttg ttgttgcagt tgctctgatt taccaggcgc tgtgccggcc agctgtttgc
    25021 gcaggctggc tacgtcggtt tcggtagtca ccatcgccat atgctcgcgc ttttgcgtca
    25081 cgctaatctt ctcaacgcct tgcggcaggt tattcaccag aatccggttg gcacgatcga
    25141 ccgcttcgcg agagtcacgg tatttgtatt gctgaccaga catatacagc gtcttatcgc
    25201 gtagctgaat ttctggcgcg tcaaagcccg cgttatactt caggtgaata agttgtatat
    25261 ttaaaatctc tttgactatc aatgaattag tataagaagg tcattattag tcaaaataaa
    25321 atcatttgtc gatttcaatt ttgtcccatt cccgtccacg gctgtcccta taccgcgccg
    25381 ccattgaatc tgatttatgt ccgagaagac gttgagcaaa cttatcgcca atctgattac
    25441 ggtatagcct cgccgacagg ctacgcagtt catggaatgt tggcgggtct ccatcaaatg
    25501 agagtcctga tgcatttctc gcctttgtaa aatacttcga tactgttttc ggggaaagcg
    25561 gatcgtgatg ctttgatgcg attatagttt cactgctgct ggcctccctg catttctgta
    25621 gtgtatcagc caatgagata ttgagcgcgt caatcgttag cgtcagcgga atggcgagct
    25681 tagcccctgt tttgctctgt tcaatgtgaa gatggttgtc gtttatgtct gaccatttca
    25741 ttctgcacaa atcgccgact ctctgccctg taacgacagc caaatccatt gctagcctca
    25801 gccagattgg gagaggttcg gctgcatggt aaatcgcgac atactcatta gctgtcagcc
    25861 ttgagcgcct tacttctgac tttgctgtgc gggttgctgt taccggattc gttgctacat
    25921 gcccctcggc tattgcctca cgaaaaacgt caacaagggt tgacctgatt aattttgcgg
    25981 aagccgcttt accttctgct acgtaggtgt ttagcattgc tgccacctct ttcgttgata
    26041 tgtcagcgag cggtttgtcc ggcaattttc ttcgga
//
LOCUS       DAEEUE010000053        25417 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.053, whole genome shotgun sequence.
ACCESSION   DAEEUE010000053 DAEEUE010000000
VERSION     DAEEUE010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25417
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.053"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            49..1388
                     /gene="yjeM"
                     /locus_tag="KLK84_002355"
                     /pseudo
     CDS             49..1388
                     /gene="yjeM"
                     /locus_tag="KLK84_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1539..4850)
                     /gene="mscM"
                     /locus_tag="KLK84_002356"
     CDS             complement(1539..4850)
                     /gene="mscM"
                     /locus_tag="KLK84_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HBE2009653.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4872..5840)
                     /gene="psd"
                     /locus_tag="KLK84_002357"
     CDS             complement(4872..5840)
                     /gene="psd"
                     /locus_tag="KLK84_002357"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HBE2009654.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5937..6989)
                     /gene="rsgA"
                     /locus_tag="KLK84_002358"
     CDS             complement(5937..6989)
                     /gene="rsgA"
                     /locus_tag="KLK84_002358"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HBE2009655.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7084..7629
                     /gene="orn"
                     /locus_tag="KLK84_002359"
     CDS             7084..7629
                     /gene="orn"
                     /locus_tag="KLK84_002359"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HBE2009656.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7840..7915
                     /locus_tag="KLK84_002360"
     tRNA            7840..7915
                     /locus_tag="KLK84_002360"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7873..7875,aa:Gly,seq:gcc)
     gene            7951..8026
                     /locus_tag="KLK84_002361"
     tRNA            7951..8026
                     /locus_tag="KLK84_002361"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7984..7986,aa:Gly,seq:gcc)
     gene            8259..8312
                     /locus_tag="KLK84_002362"
     CDS             8259..8312
                     /locus_tag="KLK84_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009657.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(8295..9434)
                     /gene="queG"
                     /locus_tag="KLK84_002363"
     CDS             complement(8295..9434)
                     /gene="queG"
                     /locus_tag="KLK84_002363"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBE2009658.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            9433..10980
                     /gene="nnr"
                     /locus_tag="KLK84_002364"
     CDS             9433..10980
                     /gene="nnr"
                     /locus_tag="KLK84_002364"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBE2009659.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            10952..11413
                     /gene="tsaE"
                     /locus_tag="KLK84_002365"
     CDS             10952..11413
                     /gene="tsaE"
                     /locus_tag="KLK84_002365"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBE2009660.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            11432..12769
                     /gene="amiB"
                     /locus_tag="KLK84_002366"
     CDS             11432..12769
                     /gene="amiB"
                     /locus_tag="KLK84_002366"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HBE2009661.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQYEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            12779..14626
                     /gene="mutL"
                     /locus_tag="KLK84_002367"
     CDS             12779..14626
                     /gene="mutL"
                     /locus_tag="KLK84_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBE2009662.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            14619..15569
                     /gene="miaA"
                     /locus_tag="KLK84_002368"
     CDS             14619..15569
                     /gene="miaA"
                     /locus_tag="KLK84_002368"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBE2009663.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMVIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            15655..15963
                     /gene="hfq"
                     /locus_tag="KLK84_002369"
     CDS             15655..15963
                     /gene="hfq"
                     /locus_tag="KLK84_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBE2009664.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            16039..17319
                     /gene="hflX"
                     /locus_tag="KLK84_002370"
     CDS             16039..17319
                     /gene="hflX"
                     /locus_tag="KLK84_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBE2009665.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            17405..18664
                     /gene="hflK"
                     /locus_tag="KLK84_002371"
     CDS             17405..18664
                     /gene="hflK"
                     /locus_tag="KLK84_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HBE2009666.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            18667..19671
                     /gene="hflC"
                     /locus_tag="KLK84_002372"
     CDS             18667..19671
                     /gene="hflC"
                     /locus_tag="KLK84_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HBE2009667.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            19753..19950
                     /locus_tag="KLK84_002373"
     CDS             19753..19950
                     /locus_tag="KLK84_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HBE2009668.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            20054..21352
                     /gene="purA"
                     /locus_tag="KLK84_002374"
     CDS             20054..21352
                     /gene="purA"
                     /locus_tag="KLK84_002374"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBE2009669.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            21557..21982
                     /gene="nsrR"
                     /locus_tag="KLK84_002375"
     CDS             21557..21982
                     /gene="nsrR"
                     /locus_tag="KLK84_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HBE2009670.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            22021..24462
                     /gene="rnr"
                     /locus_tag="KLK84_002376"
     CDS             22021..24462
                     /gene="rnr"
                     /locus_tag="KLK84_002376"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBE2009671.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            24642..25373
                     /gene="rlmB"
                     /locus_tag="KLK84_002377"
     CDS             24642..25373
                     /gene="rlmB"
                     /locus_tag="KLK84_002377"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBE2009672.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
BASE COUNT         5943 a         6457 c         7124 g         5893 t
ORIGIN      
        1 aagcacctta aaatcaccat catacactaa atcagtaagt tggcagcatt acccgcctta
       61 atgatggctg aaatgggagc tgcttatcgc aaagaagaag gtggtatcta ttcctggatg
      121 aataatagtg tcggaccacg ttttgccttc attggtacgt ttatgtggtt ttcctcttat
      181 atcatctgga tggtgagtac ctccgcgaaa gtttgggtac cgttctcaac attcctctat
      241 ggtagtgaca tgacccagca ctggcgtatt gccggactgg agcctacgca ggtggttggt
      301 ctgctggcag tagcatggat gattctggtc accgtcgttg cttcaaaggg gattaataaa
      361 attgcccgca ttactgcggt gggcggtatt gcagtaatgt gtctgaattt agtattgctg
      421 ttagtaagca ttactatttt gttattaaat ggtgggcatt tcgcgcagga tattaatttc
      481 cttgcatcac cgaacccggg ttatcagtcc ggtctggcaa tgctatcgtt tgtggtattt
      541 gctatttttg cctatggcgg aattgaagcg gttggtggtc tggtcgataa aacggaaaat
      601 ccagaaaaga actttgccaa aggtattgtt tttgccgcta ttgttatttc aatcggttat
      661 tcgctggcaa tatttttatg gggcgtcagc acaaactggc agcaggtatt aagtaatggt
      721 tccgttaacc ttggcaatat tacctatgtg ctgatgaaga gcctcggggt gacgctgggt
      781 aacgcactgc atttgtcacc tgaagcgtca ttgtcgctgg gcgtatggtt tgcgcgtatt
      841 accggacttt cgatgttcct cgcttatacc ggtgcgtttg ctattcaccg ctgaaagcca
      901 tcatccaggg gacgccgaaa gcgttgtggc cggaaccgat gacgcgcctg aatgcgatgg
      961 ggatgccgtc tatcgccatg tggatgcagt gcgggttggt tactgtcttc attctgctgg
     1021 tttcgtttgg tggcggtacc gcatcggcgt tctttaacaa gctgacgctg atggcgaacg
     1081 tgtctatgac gcttccttac ctgttcctcg cgctggcttt cccgtttttt aaagcacgtc
     1141 aggatctcga cagaccattt gtgattttca aaacgcgtat gtcggcaatg attgcgacgg
     1201 tggttgtcgt tctggtggtg acatttgcga acgtcttcac cattattcag cctgtggttg
     1261 aagccggaga ctgggacagc acattgtgga tgattggcgg ccctgtcttc ttctcgctgt
     1321 tagcgatggc gatttaccag aactattgca gtcgcatggc gaataaacct gagttagctc
     1381 tcgactgatc atatgaaatc ctgcaactca tcgtctctcc ggcgatgagt tgtcaggaag
     1441 taatgcgtat ggatgatact ctccagggcc ggtgataact gaagatgatg ttaagctgct
     1501 aaatgagcca cgttgacatc atttggcagc ttcatgcatt acaaacttcc cgcctgacga
     1561 cctttgcccg cagacgtcag cgttctcccc gtttgtttac cgttgaggct ttccagacgc
     1621 atctggaagg gcgggaatgg catatcgata ccgtgggcgt ggaagcctgc cagaatcagc
     1681 tggtggatct catggcgtag cggcatacgg tgacccatct cagcggcgta aatacgcagc
     1741 tcgaaaatct gaatcccctg ttgcagatcc accaggaaga cttccggtgc cgggttgtcg
     1801 atcaccagcg agcagcgacg cgctgcggtg agcaggattt ccgtcacttc ttcactgttg
     1861 gcatcggcag gggccggaat cgtcaacact acgcgcgtga ccgagtcaga aagcgaccag
     1921 ttgataaact gctcggtaat aaacgccttg ttcggcacga ttatctcttt acggtcccag
     1981 tcgctgatgg tggtggcgcg ggtgttaatt tttgtcacgc taccagtaag atcgcggatc
     2041 gttaccgtat cgccaatgcg aatcggtttt tcgaacagga tgatcaggcc agagataaag
     2101 ttggcgaaaa tttcctgcaa accaaaaccg agaccaacac cgagcgcggc aaccagccac
     2161 tgcaatttcg accactcaat accaatcatt gagaagccga ccagcccgcc aatcagcatc
     2221 agcagatatt ttgtgatggt ggtgatggcg taacccgtac ccggcgttaa atccaggtgc
     2281 tgcaaaatcg ccagttccag cagcgcgggc aggttgcgca ccagctgcgt ggtgatgata
     2341 aataccagaa tggcaatcag caccgcaccg agggtaattg gctccagact ttctacgccc
     2401 tgtaccgtgg aggtgacatc ccacagcgaa atattttcga ggaagccgaa agcggaatgg
     2461 atttctgacc acagcacaat gaccgaaagc agggcgatca acatcaaaat tgagcgcacc
     2521 agccgcaagg attgcgcact gatggcatcg agatcgactt cgctttcatc gacttcaatt
     2581 gctccttccg ggctactgtg atgatgcgct tcctcttcgc cacgcgcacg ttgcgctaac
     2641 atctctgccc ggcgatgctt cgcccggtca aacgccagcc tgcgacgctg gatcagcatc
     2701 cagcggcgga taacgtgata aaccaccagt agcaggaacc agatggcaac cgaggtttca
     2761 agcctcgcta acagtgcctg cgccgttgcc agataaccca ccgccgacgc cagaatggca
     2821 accagtggtg cgccaatcat catgttccac agcatatggt tggtaatgtt gtcgccgctg
     2881 ccttctttgt tgagatacaa cggaataccg gcttttttca ggcttagggt gaccaccgcc
     2941 agcgcaccgc aaatgaggat aaagcaaagc cgtcccagcg aaccggaaaa ttcgcggtcg
     3001 tcgaggttat cgaacatcat cagcgccata atcagcccga tgctcatcag gtagtagcgc
     3061 atcccacggg aaacacgttc gcgcggccag ccaaaatgag cgataaacaa gccgttcggg
     3121 cgggcaaagg tggcgcaaat catcactacc cacagcagcg gcacggtggc cgttacacca
     3181 tcgccaatcg cgaccgccag cggataaggc cacgcctcgc gcaagccgta acccagcgtc
     3241 atccacagca ccggtaacgg tgacgcgacg agaatcgacc agaaaagagt gcgcaacgtc
     3301 agccagaagt gatcctgagt cactttgccg actttcgccg ccgaacgttc aagaaaacgg
     3361 gtgaaatagc ggcgcgagta aatactgcaa ccgaccagaa tcaacgcgcc aaacagcggc
     3421 aaaattgtct ctttgctggt cagcatcatc acactggctt tgcccaactg actgaaggtg
     3481 tccagcgaaa tgagacgacg cagatcctgg gcgatttcca gcggccaggc gatggtcatc
     3541 gggcgcacgt cagaggccca gaacagatag cggtgcgttg cttcgttcac ctctttcagc
     3601 gcatcctcca gttgcccgtt ggagactttc agcttggtca gttccaatag tagcgtgtcg
     3661 ccaccctgca acaatgagtt cagcaactca cgctgagtgc gcagttgtgc ttccagaata
     3721 cggttttgct cggcggtcag cggctgaccg tcggcctgat ggatttgccg tagcagcggc
     3781 tgtttattaa gtaaatcctc ataacgtaac cgttgcacac gcaactgcgc catttcggta
     3841 tcaagctgtt gtggttttgg catttctggt agccgtgcca cctgcgcccg cagcgcttcg
     3901 ccgagcaggt tggacgatcc cagccattgc gactgttcac gcagcgtatt caacgcctgc
     3961 cggacctgta acgtctggct ggcagcctga cgctgttgcg aggcaacgag ctccatccgc
     4021 tgcgcctgtt gattcagcgc tgccgatagt tcgcggttaa ttttgaattg cgcgacgata
     4081 tctttcggca aatcggcact gttttctgcc agtaattcgg tactttccag cgcccgctcc
     4141 gcctcaagct gacgttggct attcaactga ttacgtaagg cctgcaaata cgcatccagt
     4201 tgctggctct ctttttccgc cagctctgag cgtaagcgcg ctaattcctg gcggttattg
     4261 gcagacagct gcgccagctc cagttcatca acgagcgcct taagacgtgc agagtcagac
     4321 tgcaacgcga aattttgtgc ctgattgagc ggagtattgc cggtaagcgt tcccaggcgg
     4381 cgctcgatct catttaactg acggcgggcg tcggtttgct gttgcggcag ttgattcagc
     4441 gaatcggcaa tctcgcgggc tcgctcctgc tcttgctggg cctgacggct tttatccagc
     4501 aactggctgc tgacctggag aatttcctga ttcagcgcgt cggtagacat tcccggcgac
     4561 acgctgcgcg gctcgtcacg aatgttgttt aattgtgcgc gcagagtagc ggagagtttc
     4621 ggataattat cgataacttg ctgatattgt ttgatgcgct caagggaacc ttttcgttcc
     4681 tcaagcgcat ttaaggcaga ctggagcgcc tctacgactt ccggctgtgc gggtttcgcc
     4741 gcttttgcct gctccagttc ctgagtgatt tgtttgctat cgggggccgt cgcggcgtac
     4801 gccccccaac tgaggcacca ggccatcaga aaagtgataa tcaggcgcac gtcagcgttt
     4861 cctttgatgg attagacctg gtctttttta tcgtcaacca atgggctggc gtcgtgttct
     4921 gcttcgatct cttcagcagg aagtggggcg ggttcagcgt ctggcgtaac aaaggtttcg
     4981 gtagatactg ccagcggctg gccaattttc gtgacagaca ggctttccag ttgctcaacc
     5041 agattcactt tacccggtgc aaacaggttg ataacggtgg aaccgagttt aaagcgaccc
     5101 atttcctggc ctttcagcag tgccacagaa ccgtcgtttt ccccggcagg ccaggtccag
     5161 cgcttgatga taccttcgcg cggcggcgta atggtgcccg cccagaccgt ctcaatgctg
     5221 ccaacaatcg tcgctccgac cagaatctgc gccattgggc caaattcggt atcgaaaagg
     5281 caaatcacgc gttcgttacg ggcaaacaga ttcggcacgt tctgagcggt gagatggtta
     5341 acggaaaaga gatcgcccgg cacgtagatc atctcacgca gaataccgtt gcacggcatg
     5401 tgtacgcgat ggtagtcacg cggggagagg taagtggtca caaacgtacc gttgcggaac
     5461 aggtccgcca tcagatagtt gcctgccagc agggcttcga ggctgtagtt gtggcctttg
     5521 gcttgcagga ttttatcttc ttcgatttta cccaactggc tgataacgcc atcggcaggc
     5581 atgaccagta cattcggatc ggtatcgatt gggcgtactt cgtcacgcag cggacggaca
     5641 aagaattcgt taaaggtgcg gtagctggcg gtgtccggct tttgcgcctc tttcatgtcg
     5701 accttgtagt atttaacgaa cagatcgata accagttttg tcagccatcc tgcccgcttg
     5761 cttgcgcccc aacccgccag gcgagtaagc catagtttcg gcagaatgta ctgtagcgaa
     5821 agtttaaatg aatttaacaa ggtagcctcc aggccattgt tttgtcgttc ctgatccggc
     5881 caacatgccg gatcctgaaa aaaaggggac gattctaacg acggttagct taattgtcag
     5941 tcatccgtat cagaaaagtt tttacgcgtt tttacctgcg ccatgctttc cagaatacgg
     6001 tgatagtttt cgaaacgggt ttccgcgatt ttcccttcct caaccgcttc ccggatagcg
     6061 cagcccggat cggtatcgtg tttgcaatcg cgatatttac acagacctaa atagtcatgg
     6121 aattcgacaa agccctgagt gatttgttcc ggctccaggt gccagaggcc gaactcacgc
     6181 acaccagggg aatcaatcac atcaccgccg tgcgggaagt gatacagccg agcggcggtc
     6241 gtggtgtgct gaccgagccc cgagttgtca gagacatcgt tggtcaggat ctctttttgc
     6301 agccccagca acgcgttcag caggctggat ttaccgacgc cagactgccc ggcaaaaatg
     6361 ctgatgcgcc cggtcaacgc ctcttccagc ggtttcagcc catcctgagt atggctggaa
     6421 accatcaata cgcgataacc gatgttgcgg tagatatcca tctgctcgtt gacgaacgcc
     6481 atgccttcgt cgtccagcag gtctatcttg ttgagcacga taatcggctc aatctgcaag
     6541 gtttcgcagg ccaccaggta acggtcgata atattgagcg acagctccgg caaaatggcg
     6601 gagacaatga caatctggtc gatgttggcg gctatgggtt tcacgccgtc gtagaaatcc
     6661 ggacgcgtta acaccgaggt gcgctcatgc accgcttcca cgatcccttt gacatttacg
     6721 ccttccgccg ccggtttacc cggacgccag actacgcggt cgccggttac cagcgaacgg
     6781 atggtacggc gaatattgca gcggtgaacg tcgccatcgg cggattccac atcagcgtgc
     6841 ataccaaagc ggctgatgac gataccttca tcaggctcgc caaacagatt gtcgtcgtag
     6901 tcgggcttct ccttagacgt tttaagacga cgctggtgat tggcgttcac gcggcgctgc
     6961 tggcctttgg agagtttatt tttactcaat cgtactgact cctggtcgcc cctgatgggc
     7021 aaaacatcta tgatacacgc aattgtggat caatatagtc actgtgaatg ggtggaaaat
     7081 agcatgagtg ccaatgaaaa caacctgatt tggatcgatc ttgagatgac cggtctggat
     7141 cccgagcgcg atcgcattat tgagattgcc acgctggtga ccgatgccaa tctgaatatt
     7201 ctggcagaag ggccgaccat tgcagtacat cagtccgatg aacagctggc gctgatggat
     7261 gactggaacg tgcgcaccca taccgccagc gggctggtag agcgcgtgaa agcgagcacg
     7321 atgggcgatc gggaagctga actggcaacg ctcgaattct taaaacagtg ggtgcctgcg
     7381 ggaaaatcgc cgatttgcgg taacagcatc ggacaggacc gtcgtttcct gtttaaatac
     7441 atgccggagc tggaagtcta cttccactac cgttatctcg atgtcagcac cctgaaagag
     7501 ctggcgcgcc gctggaagcc ggaaattctg gatggtttta ccaagcaggg gacgcatcag
     7561 gcgatggatg atatccgtga atcggtggcg gagctggctt actaccgcga gcattttatc
     7621 aagctgtaaa atttttcctg gtcacgtaag cgccggaatc cacttgcggc ggcgtgattt
     7681 tgacgctaaa tcgaacgtct tgctgataat ttcagcactt gagataaaaa cgcaaaaaaa
     7741 acttttttgg ggggttgcag agggaaagat ttctcgtata atgcgcctcc cgtaacgacg
     7801 cagaaatgcg aaaattacga aagcaaaatt aagtagtacg cgggaatagc tcagttggta
     7861 gagcacgacc ttgccaaggt cggggtcgcg agttcgagtc tcgtttcccg ctccaaaatt
     7921 taaaagtgct gtaaggcaca gaccacccaa gcgggaatag ctcagttggt agagcacgac
     7981 cttgccaagg tcggggtcgc gagttcgagt ctcgtttccc gctccaaatt cttctctcaa
     8041 taaaatatct acagcgacgc gatgcgttat tgctggtttt tgttgtctct gacaaactct
     8101 tgtaaacaga gttatccaca gcctcaggct gtaatcttaa tttcaaagaa acttcgcacg
     8161 gtgaatggta ttttttaacc tattgataaa taagttaaaa atcaagattc cgttttgtcg
     8221 agtcgatcac ttgacgattt tattcgtctt gaattgcaat gcgtttttat ttttattcac
     8281 aagctgtgga tgaatcaggc gtcacgcggt aacccttttt caatcacccg aaccagacgc
     8341 tgttttttcg gcaattgcac ttcgactatg cacgcatttc gtctctctat ttgctgcgca
     8401 atcgcccacg ctatgtgctc atcgagaagt gggtgctcac ctttacgact ttccagcgct
     8461 gtcaaaatcg tttcatccca aggggcattg cctaatgcca cggcgatatt acgcagccag
     8521 cgcaggtgac ctatacggcg aatcgccgat ccttccgtga cttttaaaaa cttctcttcg
     8581 ctccaggcaa ataactcaat gagttccggt gcgtgtagcg gcttacgcgg gctgaaatcg
     8641 tcttctgtag tgagttgcga atagcgattc cacgggcaga taagctggca gtcatcgcaa
     8701 ccgtaaatac ggtttcccat taacggtcgc aactcttccg ggagcgcccc ttcaagttcg
     8761 atggtgagat aagagataca gcggcgagca tcgacggtat atggctcgac gatggcaccg
     8821 gtcgggcaaa tcgtcataca ggccacgcat ttgccgcatc cttcctcgac tggttgatcc
     8881 acgggcagcg gaatatcgac cagcaattcg cctaaaaaga agaacgaacc ggcctcgcga
     8941 ttgaggataa gtgagtgctt acctgtccag ccgagcccag ctttttcagc taacgggcgc
     9001 tcgagaatag gcgcagaatc gacaaacggt ctaaaattca gcgaaacaca atgttgctga
     9061 atcatctcgc ccagcttttt gagtcggttg cgcagaagtt tgtgatagtc acggcccagc
     9121 gcataacggc taacatagcc gagtttgggg tttttcagcg tgctggcaaa tgcggcgtta
     9181 gcaggaaggt aattcatccg cacgctgatt acacgcagcg taccgggtaa taactcatgg
     9241 gggcgagcgc gcagcatacc gtgacgtgcc atccagtcca tttcgccgtg gtattgtttg
     9301 tccagccatg cttgcagttt gggctcggac tcgctgagat cggtatcggt aatacctacc
     9361 tgctgaaagc ccagttccag cccccactgt ttaatttttt gcgctaactg attgagatcg
     9421 aggggctctg acatgacgga ccatacaatg aagaaaaacc ccgtaagtat accacacacc
     9481 gtctggtacg ccgacgatat ccgccgcgga gaacgcgagg cggcagatgt gctggggctc
     9541 acactctatg agctgatgct tcgcgctggc gaggccgcat tccaggtgtg tcgttcggcg
     9601 tatcctgacg cccgccactg gctggtgctg tgcggtcatg gtaataacgg cggcgatggc
     9661 tacgtggtcg cgcgactggc caaagcggtc ggcattgagg tcacgttgtt ggcccaggag
     9721 agcgacaaac cgttgccgga agaggccgcg ctggcacgcg aagcatggtt aaacgcgggt
     9781 ggcgagatcc atgcttcgaa tattgtctgg cccgaatcgg tagatctgat tgttgatgcg
     9841 ctgctcggta ccggcttgca gcaagcgccc cgcgaatcca ttagccagtt aatcgaccac
     9901 gctaattccc atcctgcgcc gattgtggcg gttgatatcc cttccggcct gctggctgaa
     9961 accggcgcta cgccaggcgc agtgatcaac gccgatcaca ccatcacttt tattgcgctg
    10021 aaactaggct tgctcactgg aaaagcgcgg gatgttacag gacaactgca ttttgactca
    10081 ctggggctgg atagctggct ggcaggtcag gagacgaaaa ttcagcggtt ttcggcagaa
    10141 caactttctc actggctgaa accgcgtcgc ccgacttcgc ataaaggcga tcacgggcgg
    10201 ctggtgatta tcggtggcga tcacggcacg gcgggggcta ttcgtatgac gggggaagcg
    10261 gcgctacgtg ctggtgctgg tttagtccga gtactgaccc gcagtgagaa cattgcgccg
    10321 ctgctgactg cacgaccaga attgatggtg catgaactga ctatggactc tcttaccgaa
    10381 agcctggaat gggccgatgt ggtggtgatt ggtcccggtc tgggccagca agagtggggg
    10441 aaaaaagccc tgcaaaaagt tgagaatttt cgcaaaccga tgttgtggga tgccgatgca
    10501 ttgaacctgc tggcaatcaa tcccgataag cgtcacaatc gcgtgatcac gccgcatcct
    10561 agcgaggccg cacggttgtt aggctgttcc gtcgctgaaa ttgaaagtga ccgcttacat
    10621 tgcgccaaac gtctggtaca acgttatggc ggcgtagcgg tgctgaaagg tgccggaacc
    10681 gtggtcgccg cccatcctga cgctttaggc attattgatg ccggaaatgc aggcatggcg
    10741 agcggcggca tgggcgatgt gctctctggt attattggcg cattgcttgg gcaaaaactg
    10801 tcgccgtatg atgcagcctg tgcaggctgt gtcgcgcacg gtgcggcagc tgacgtactg
    10861 gcggcgcgtt ttggaacgcg cgggatgctg gcaaccgatc tcttttccac gctacagcgt
    10921 attgttaacc cggaagtgac tgataaaaac catgatgaat cgagtaattc cgctccctga
    10981 tgagcaggca acattagacc tgggcgagcg ggtagcgaaa gcctgcgatg gcgcaaccgt
    11041 aatctatctg tatggcgatt taggcgcagg taaaaccacc tttagccggg gctttttaca
    11101 ggctctgggt catcagggta atgtcaaaag ccccacttat acgctggtcg aaccctatac
    11161 gctcgacaac ttaatggtct atcactttga tttgtaccgc cttgccgatc ccgaggagct
    11221 ggagtttatg gggatccgcg attattttgc caacgatgcc atctgcctgg tggagtggcc
    11281 acaacaaggt acaggtgttc ttcctgaccc ggatgtcgaa atacacattg attatcaggc
    11341 acaaggccgt gaggcgcgcg tgagtgcggt ttcctctgcg ggtgaattgt tgctggcgcg
    11401 tttagccggt taacctttga aaggtggcgg gatgatgtat cgcatcagaa attggttggt
    11461 agcgacgctg ctgctgctgt gcacgccggt gggtgccgcg acgctctctg atattcaggt
    11521 ttctaacggt aatcaacagg cgcggataac gttgagtttt attggcgatc ctgattatgc
    11581 gtttagccat caaagcaaac gcaccgtggc gctcgatatc aaacaaacgg gcgtgattca
    11641 gggactgccg ttgttgttca gcggcaataa tctggtgaag gcgattcgct ctggaacgcc
    11701 taaagatgca caaacgctac ggctggtggt cgatcttacc gaaaacggta aaaccgaagc
    11761 ggtgaagcgg cagaatggca gcaattacac tgtcgtcttt acgattaacg ccgatgtgcc
    11821 gccaccgcct cctccgccgc ccgtggttgc gaaacgcgtt gaaacgcctg cggttgtcgc
    11881 accgcgcgtc agcgaaccgg cgcgcaatcc gtttaaaacg gaaagtaacc gcactacggg
    11941 tgttatcagc agtaatacgg taacgcgtcc ggcagcgcgc gcgacggcta acactggcga
    12001 taaaattatc atcgctattg atgccggaca cggcggtcag gaccctggcg ctatcggccc
    12061 cggtggtacg cgggagaaaa atgtcaccat cgccatcgcg cgtaaattgc gtactttgct
    12121 caatgacgat ccgatgttta aaggcgtttt aacccgtgac ggggattact ttatctcggt
    12181 gatggggcgc agtgatgtgg cacataagca aaacgccaat ttcctcgtgt cgattcacgc
    12241 tgatgccgca ccgaaccgta gtgcgactgg cgcttccgta tgggtgctct ctaaccgtcg
    12301 cgccaacagt gaaatggcca gctggctgga gcagtacgag aaactgtcgg agctgctggg
    12361 tggggcgggt gatgtgctgg cgaacagtca gtctgacccc tatttaagcc aggcggtgct
    12421 ggatttacag ttcggtcatt cccagcgggt agggtatgat gtagcgacca gtatgatcag
    12481 tcagttgcaa cgcattggcg aaatacataa acgtcgacca gaacacgcca gccttggcgt
    12541 tctgcgctcg ccggatatcc catcagtact ggtcgaaacc ggttttatca gcaacaacag
    12601 cgaagaacgt ttgctggcga gcgacgatta ccaacaacag ctggcagaag ccatttacaa
    12661 aggcctgcgc aattacttcc ttgcgcatcc gatgcaatct gcgccgcagg gtgcaacggc
    12721 acaaactgcc agtacggtga cgacgccaga tcgcacgctg ccaaactaag gacgattgat
    12781 gccaattcag gtcttaccgc cacaactggc gaaccagatt gccgcaggtg aggtggtcga
    12841 gcgacctgcg tcggtagtca aagaactagt ggaaaacagc ctcgatgcag gtgcgacgcg
    12901 tatcgatatt gatatcgaac gcggtggggc gaaacttatc cgcattcgtg ataacggctg
    12961 cggtatcaaa aaagatgagc tggcgctggc gctggctcgt catgccacca gtaaaatcgc
    13021 ctctctggac gatctcgaag ccattatcag cctgggcttt cgcggtgagg cgctggcgag
    13081 tatcagttcg gtttcccgcc tgacgctcac ttcacgcacc gcagaacagc aggaagcctg
    13141 gcaggcctat gccgaaggac gcgacatgga cgtgacggtt aaaccggcag cgcatccggt
    13201 gggaacgacg ctggaggtgc tggatctgtt ctacaacacc ccggcgcggc gcaaattcct
    13261 gcgcaccgag aaaaccgaat ttagccatat tgatgaaatc atccgccgca ttgcgctggc
    13321 gcgtttcgac gtcacgatca acctgtcgca taacggtaaa attgtgcgtc agtaccgcgc
    13381 agtgccggaa ggcgggcaaa aagaacggcg cttaggcgcg atttgcggca ccgcttttct
    13441 cgaacaagcg ctggcgattg aatggcaaca cggcgatctc accctgcgcg gctgggtggc
    13501 cgatccaaat cacactacgc ccgcactggc ggaaattcag tattgctacg tgaatggtcg
    13561 catgatgcgc gatcgcctga tcaatcatgc gatccgccag gcctgcgaag acaaactggg
    13621 ggccgatcag caaccggcat ttgtgttgta tctggagatc gacccgcatc aggtggacgt
    13681 caacgtacac cccgccaaac acgaagtgcg tttccatcag tcgcgtctgg tgcatgattt
    13741 tatctatcag ggcgtgctga gcgtgctaca acagcaactg gaaacgccgc taccgctgga
    13801 cgatgaacca caacccgcac cgcgtgccat cccggaaaac cgcgtggcgg cggggcgcaa
    13861 tcactttgca gaaccggcag ctcgtgagcc ggtagctccg cgctactctc ctgcgcccgc
    13921 ctcaggcagt cgtccggctg ccccctggcc gaatgcgcag ccaggctacc agaaacagca
    13981 aggtgaagtg tatcgccagc ttttgcaaac gcccgcgccg atgcaaaaac caaaagcgcc
    14041 ggaaccgcag gaacctgcac ttgcggcgaa cagtcagagt tttggtcggg tactgactat
    14101 cgtccattcc gactgtgcgt tgctggagcg cgatggcaac atttcacttt tatccttgcc
    14161 agtggcagaa cgttggctgc gtcaggcaca attgacgccg ggtgaagcgc ccgtttgcgc
    14221 ccagccgctg ctgattccgt tgcggctaaa agtttctggc gaagaaaaat cggcattaga
    14281 aaaagcgcag tctgccctag cggaattggg tattgatttc cagtcagatg cacagcatgt
    14341 gaccatcagg gccgtgcctt tacccttacg ccaacaaaat ttacaaatct tgattcctga
    14401 actgataggc tacctggcga agcagtccgt attcgaacct ggcaatattg cgcagtggat
    14461 tgcacgaaat ctgatgagcg aacatgcgca gtggtcaatg gcacaggcca taaccctgct
    14521 ggcggacgtg gaacggttat gtccgcaact tgtgaaaacg ccgccgggtg gtctgttaca
    14581 atctgttgat ttacatccgg cgataaaagc cctgaaagat gagtgatatc agtaaggcga
    14641 gcctgcctaa ggcgattttt ttgatggggc cgacggcctc cggtaaaacg gcgttagcca
    14701 ttgagctgcg taaaatttta ccagtagagt tgataagcgt tgattctgcc cttatttaca
    14761 aagggatggt tatcgggacg gcgaagccga acgctgaaga gttactcgcc gcgccgcacc
    14821 gattgctgga tattcgcgat ccgtcgcagg cttactcggc tgctgatttt cgccgcgatg
    14881 cgctggcgga aatggccgat atcaccgcgg cggggcggat cccactgtta gtgggcggta
    14941 cgatgttgta tttcaaggca ttgctggaag ggttgtcgcc gctaccgtcg gcagacccgg
    15001 aagtgcgggc cagaattgag caacaggcgg cagagcaagg ttgggagtca ttgcatcgtc
    15061 aacttcagga ggtagatccg gttgcggcag caaggattca tccaaatgat ccacaaaggc
    15121 tttcccgggc actggaagtt tttttcattt cgggtaaaac tttaacggaa ctgacgcaaa
    15181 cgtcaggaga cgctctaccg tatcaggtgc atcagttcgc catcgccccg gcgagccgtg
    15241 aactgctcca tcaacgcatt gagcagcgtt ttcatcagat gttggcttca ggttttgaag
    15301 cagaagtccg ggcgcttttt gcccgaggag atttgcatac ggacttgcct tccattcgtt
    15361 gcgtgggtta tcgccagatg tggtcttacc ttgaaggcga aatctcatac gatgaaatgg
    15421 tttatcgagg tgtttgcgcc acgagacagt tggcgaagcg gcagataacc tggctgcgtg
    15481 gttgggaagg ggttcactgg cttgacagtg aaaaaccaga acaggcgcgt gacgaagtat
    15541 tacaggttgt tggtgctatc gcaggctgaa tgtgtacaat tgagacgtat cgtgcgcaat
    15601 tttttcagaa tcgaaaggtt caaagtacaa ataagcatat aaggaaaaga gagaatggct
    15661 aaggggcaat ctttacaaga tccgttcctg aacgcactgc gtcgggaacg tgttccagtt
    15721 tctatttatt tggtgaatgg tattaagctg caagggcaaa tcgagtcttt tgatcagttc
    15781 gtgatcctgt tgaaaaacac ggtcagccag atggtttaca agcacgcgat ttctactgtt
    15841 gtcccatctc gcccggtttc tcatcacagt aacaacgccg gtggcggtac cagcagtaac
    15901 taccatcatg gtagcagcgc gcagaatact tccgcgcaac aggacagcga agaaaccgaa
    15961 taaggtttcg ggctgttttt ttacacgggg agccagcgat cctgcgttcc ccgctgatct
    16021 atttagaggg ttatacgctt gtttgaccgt tatgatgctg gtgagcaggc ggtactggta
    16081 cacatctatt ttacgcaaga caaagatatg gaagacctcc aggagtttga atctctggtc
    16141 tcttccgccg gtgtcgaagc attgcaggtg attaccggta gccgtaaagc gccgcaccca
    16201 aagtattttg taggtgaagg taaagcagtt gaaattgcgg aagctgtcaa agcgatgggt
    16261 gcttcggtcg ttctttttga ccatgccctg agcccggcgc aagagcgtaa cctggagcgt
    16321 ttgtgcgagt gtcgtgttat cgaccgcacc ggccttattt tagatatttt cgcccaacgt
    16381 gcgcgtaccc atgagggtaa gttgcaggtt gagctggcgc agctgcgcca tctggctacg
    16441 cgcctggtgc gtggctggac ccaccttgaa agacagaaag gcgggatagg tttgcgtggt
    16501 ccgggtgaaa cccagctcga aaccgaccgt cgtttgttgc gtaatcgcat cgtgcagata
    16561 cagtcgcgcc tggaaagagt tgaaaagcag cgtgagcagg ggcggcaatc gcgtatcaaa
    16621 gccgacgttc ctactgtttc gctggtggga tataccaacg ccggtaaatc tacccttttc
    16681 aatcgcatca ccgaagcgcg ggtctacgcg gcagaccagt tgtttgccac cctcgacccg
    16741 acgttgcggc gtattgacgt tgcagatgtc ggtgaaaccg tacttgcaga taccgtaggg
    16801 tttattcgcc acctgccgca cgatctggtg gcggcattta aagccacgtt acaagagacg
    16861 cggcaagcca cattactgct gcacgtcatt gatgcggcgg atgtgcgtgt acaagaaaac
    16921 atcgaagcgg tgaatacggt tcttgaagag atcgacgctc acgagatccc aaccctgctg
    16981 gtgatgaaca agatcgatat gctggaagat ttcgaaccgc gtattgatcg ggacgaagag
    17041 aacaaaccga tccgtgtctg gctttccgca cagaccggag cggggatacc acagcttttt
    17101 caggctttga cggagcggct ttccggcgag gtggcgcagc atacattgcg tctgccaccg
    17161 caggaagggc gtctgaggag tcgtttttat cagcttcagg caatagaaaa agagtggatg
    17221 gaggaggacg gcagcgtaag tctgcaagtt cgtatgccga tcgttgactg gcgtcgcctc
    17281 tgtaaacaag aaccggcgtt gatcgattac ctgatctaac ggcgtagcgt ctgaagcgtg
    17341 gagtcatatc ctctggcgtc gaaagacaac agggatcacc gcataacaaa tatggagcac
    17401 aaacatggcg tggaatcagc ccggtaataa cggacaagac cgcgacccgt ggggaagcag
    17461 caaacctggc ggcaactctg agggaaatgg aaacaaaggc ggtcgcgatc aagggccacc
    17521 tgatttagat gatatcttcc gcaaactgag caaaaagctc ggtggtctgg gcggcggtaa
    17581 aggcaccgga tctggcggtg gcagttcatc gcaaggcccg cgcccgcagc ttggcggtcg
    17641 tgtcgttacc atcgcagcgg cagcgattgt cattatctgg gcggccagtg gtttctatac
    17701 cattaaagaa gccgaacgcg gcgtggtaac acgctttggt aaattcagcc atctggttga
    17761 gccgggtctg aactggaaac cgacgtttat cgacgaagtc aaaccggtga acgtggaagc
    17821 cgtgcgtgaa ctggccgctt ctggtgtgat gctgacgtcg gacgagaacg tagtgcgcgt
    17881 tgagatgaac gtgcagtacc gcgtcaccaa tccggaaaaa tatctgtata gcgtgaccag
    17941 cccggatgac agcctgcgtc aggctaccga cagcgccctg cgtggagtta tcggtaaata
    18001 caccatggac cgcattctga cggaaggtcg taccgtgatt cgtagcgata ctcagcgcga
    18061 actggaagag acgattcgtc cgtatgacat gggtatcacg ctgctggacg tcaacttcca
    18121 ggctgctcgt ccgccggaag aagtaaaagc ggcgtttgac gatgcgattg ccgcgcgtga
    18181 aaacgaacag caatacattc gtgaagcaga agcgtatacc aacgaagttc agccgcgtgc
    18241 gaacggtcag gcgcaacgta tcctcgaaga ggcgcgtgcg tacaaggccc agaccatcct
    18301 ggaagctcag ggtgaagtgg cgcgctttgc taaacttctg ccggaatata aagccgcgcc
    18361 ggaaattact cgcgagcgtc tgtatatcga gacgatggaa aaagtgttgg gtaacacccg
    18421 caaagtgctg gttaacgata aaggtggcaa cctgatggtt ctgccgttag accagatgct
    18481 gaaaggtggt aacgcccctg cggcgaagag cgataacggt gccagcaatc tgctgcgtct
    18541 gccgccagcc tcttcctcca caaccagtgg agcaagcaac acgtcgtcca ccagtcaggg
    18601 cgatattatg gaccaacgcc gcgccaacgc gcagcgtaac gactaccagc gtcaggggga
    18661 ataacgatgc gtaagtcagt tatcgcgatt atcatcatcg tgctggtagt gctttacatg
    18721 tctgtctttg tcgtcaaaga aggtgagcgc ggtattacgc tgcgttttgg taaggtactg
    18781 cgtgacgatg acaacaaacc tttggtttat gagcctggtc tgcatttcaa gataccgttc
    18841 attgaaacgg tgaaaatgct cgacgcacgt attcagacca tggacaacca ggccgaccgc
    18901 tttgtgacca aagagaagaa agacctgatc gtcgactctt acatcaaatg gcgcatcagc
    18961 gatttcagcc gttactacct ggcaacgggt ggtggcgaca tttcgcaagc ggaagtgctg
    19021 ttgaaacgta agttctctga ccgtctgcgt tctgaaattg gtcgcctgga cgtgaaagat
    19081 atcgtcaccg attcccgtgg tcgtctgacc ctcgaagtac gtgacgcgct gaactccggt
    19141 tctgcgggta cagaagatga agttactacc ccggcagcag ataacgccat tgccgaagcg
    19201 gcagagcgcg taacggctga gacgaagggc aaagttccgg tcatcaaccc gaacagtatg
    19261 gcggcgctgg gtattgaagt tgtcgatgtg cgtatcaagc agatcaacct gccgaccgaa
    19321 gtgtctgaag cgatctacaa ccgtatgcgc gccgagcgtg aagcggtagc gcgtcgtcac
    19381 cgttcacaag gtcaggaaga agcggaaaaa ctgcgcgcga ctgccgacta tgaagtgacc
    19441 agaacgctgg cagaagctga gcgtcagggc cgcattatgc gtggtgaagg cgatgccgaa
    19501 gcagccaaac tgtttgctga tgcattcagt aaagatccgg acttctacgc attcatccgt
    19561 agcctgcgtg cttatgagaa cagcttctct ggcaatcagg acgtgatggt catgagcccg
    19621 gatagcgatt tcttccgcta catgaagacg ccgacttccg caacgcgtta atataacgac
    19681 tgcggtacag gtcaataaag ccaccgcatc ctcagggatg tcggtggttt tctttttcta
    19741 taaggataat gaatgaattc gacaatctgg ctggcgcttg ccctggtttt ggtactggaa
    19801 ggtttagggc cgatgcttta cccgaaggca tggaagaaga tgatctctgc gatgaccaat
    19861 ttgcccgata atattttacg tcgttttggc ggtggacttg tggttgcggg cgttgtggtc
    19921 tactacatgt tgaggaaaac gattggctga acaaaaaaca gactgatcga ggtcattttt
    19981 gagtgcaaaa agtgctgtaa ctctgaaaaa gcgatggtag aatccatttt taagcaaacg
    20041 gtgattttga aaaatgggta acaacgtcgt cgtactgggc acccaatggg gtgacgaagg
    20101 taaaggtaag atcgtcgatc ttctgactga acgggctaaa tatgttgtac gctaccaggg
    20161 cggtcacaac gcaggccata ctctcgtaat caacggtgaa aaaaccgttc tccatcttat
    20221 tccatcaggt attctccgcg agaatgtaac cagcatcatc ggtaacggtg ttgtgctgtc
    20281 tccggccgcg ctgatgaaag agatgaaaga actggaagac cgtggcatcc ccgttcgtga
    20341 gcgtctgctg ctgtctgaag catgtccgct gatccttgat tatcacgttg ctctggataa
    20401 cgcgcgtgag aaagcgcgtg gcgcgaaagc gatcggcacc accggtcgtg gtatcgggcc
    20461 tgcttatgaa gataaagtgg cacgtcgcgg tctgcgtgtt ggcgaccttt tcgacaaaga
    20521 aaccttcgct gaaaaactga aagaagtgat ggaatatcac aacttccagt tggttaacta
    20581 ctacaaagct gaagcggttg attaccagaa agttctggat gatacgatgg ctgttgccga
    20641 catcctgact tctatggtgg ttgacgtttc tgacctgctc gaccaggcgc gtcagcgtgg
    20701 cgatttcgtc atgttcgaag gtgcgcaggg tacgctgctg gatatcgacc acggtactta
    20761 tccgtacgta acttcttcca acaccactgc tggtggcgtg gcgaccggtt ccggcctggg
    20821 cccgcgttat gttgattacg ttctgggtat cctcaaagct tactcaactc gtgtaggtgc
    20881 aggtcctttc ccggccgaac tgtttgatga aactggcgag ttcctctgca agcagggtaa
    20941 cgaattcggt gcaactacgg gtcgtcgtcg tcgtaccggc tggctggaca ccgttgccgt
    21001 tcgtcgtgcg gtacagctga actccctgtc tggcttctgc ctgactaaac tggacgttct
    21061 ggatggcctg aaagaggtta aactctgcgt ggcttaccgt atgccggatg gtcgcgaagt
    21121 gactaccact ccgctggcag ctgacgactg gaaaggtgta gagccgattt acgaaaccat
    21181 gccgggctgg tctgaatcca ctttcggcgt gaaagatcgt agcggcctgc cgcaggcggc
    21241 gctgaactac atcaagcgta ttgaagagct gaccggtgtg ccgatcgata tcatctctac
    21301 cggtccggat cgtactgaaa ccatgattct gcgcgacccg ttcgacgcgt aattctggta
    21361 cgcctggcag atattttgcc tgccgggcta acagtgtgat acattgctgt gtcggttaag
    21421 ccattacgct atccgacaca gtgttaaatc ctcgcttttt tccttccccg aactgaaata
    21481 aattagcgac acagcttgtg gctggtttat catcaatata aatgtatttt ttcccgattt
    21541 cccttttgag gttgatgtgc agttaacgag tttcactgat tacggattac gtgcgctgat
    21601 ctacatggcg tcattgccag aagggcggat gaccagtatt tctgaagtga ctgacgtcta
    21661 cggcgtctcc cgtaatcata tggtcaaaat aatcaatcaa cttagtcgtg ccggctacgt
    21721 gactgctgtt cgtggaaaaa atggcggcat tcgcctgggt aaaccggcga gtgcgatacg
    21781 tattggtgat gtggtgcgcg agctggagcc cttatcgctg gtgaattgca gcagtgagtt
    21841 ttgccacatt acacctgcct gtcggttgaa acaggcgctt tctaaggccg tgcaaagttt
    21901 tcttacggaa ctggataact acacgcttgc cgatttggtt gaagagaatc aaccgcttta
    21961 taaattattg ctggtggagt gacgaaaatc ttcatcagag atgacaacgg aggaaccgag
    22021 atgtcacaag atcctttcca ggaacgcgaa gctgaaaaat acgcgaatcc catccctagt
    22081 cgggaattta tcctcgaaca tttaaccaaa cgtgaaaaac cggccagccg tgatgagctg
    22141 gcggtagaac tgcacattga aggcgaagag cagcttgaag gcctgcgtcg ccgcctgcgc
    22201 gcgatggagc gcgatggtca actggtcttc actcgtcgtc agtgctatgc gctgccggaa
    22261 cgcctcgacc tggtgaaagg taccgttatt ggtcaccgtg atggctacgg ctttctgcgg
    22321 gttgaagggc gtaaagatga tttgtatctc tccagcgagc agatgaaaac ctgcattcat
    22381 ggcgatcagg tgctggcgca gccgctgggt gctgaccgta aaggtcgtcg tgaagcgcgt
    22441 attgtccgcg tactggtgcc aaaaaccagc cagattgttg gtcgctactt taccgaagcg
    22501 ggcgtcggct ttgtggttcc tgacgatagc cgtctgagct tcgatatctt aatcccgccc
    22561 gatcagatca tgggcgcgcg gatgggcttt gtggtcgtag tcgaactgac tcagcgtccg
    22621 actcgccgca ccaaagcggt gggtaaaatc gtcgaagtgc tgggcgacaa tatgggcacc
    22681 ggcatggcgg ttgatatcgc tctgcgtacc catgaaattc cgtatatctg gccgcaggct
    22741 gttgagcaac aggttgcggg gctgaaagaa gaagtgccgg aagaagcaaa agcgggccgt
    22801 gtcgatttgc gcgatttacc gctggtcacc attgatggcg aagacgcccg tgactttgac
    22861 gatgcagttt actgcgagaa aaaacgcggc ggcggctggc gtttatgggt cgcgattgcc
    22921 gacgtcagct actatgtgcg tccgccaacg ccgctggaca gagaagcgcg taaccgtggc
    22981 acgtcggtgt acttcccttc gcaggttatc ccgatgctgc cggaagtgct ctctaacggc
    23041 ctgtgttcgc tcaacccgca ggtagaccgc ctgtgtatgg tgtgcgagat gacggtttcg
    23101 tcgaaaggcc gcctgacggg ctacaaattc tacgaagcgg tgatgagctc tcacgcgcgt
    23161 ctgacctaca ccaaagtctg gcatattctg cagggcgatc aggatctgcg cgagcagtac
    23221 gccccgctgg ttaagcatct cgaagagttg cataacctct ataaagtgct ggataaagcc
    23281 cgtgaagaac gcggtgggat ctcatttgag agcgaagaag cgaagttcat tttcaacgct
    23341 gaacgccgta ttgaacgtat cgaacagacc cagcgtaacg acgcgcacaa attaattgaa
    23401 gagtgcatga ttctggcgaa tatctcggcg gcgcgtttcg ttgagaaagc gaaagaaccg
    23461 gcactgttcc gtattcacga caagccgagc accgaagcga ttacctcttt ccgttcagtg
    23521 ctggcggagc tggggctgga gctgccgggt ggtaacaagc cggaaccgcg tgactacgcg
    23581 gaactgctgg agtcggttgc cgaccgtcct gatgcagaaa tgctgcaaac catgctgcta
    23641 cgctcgatga aacaggcgat ttacgatcca gaaaaccgtg gtcacttcgg tctggcattg
    23701 cagtcctatg cgcactttac ttcgccgatt cgtcgttatc ctgacctgac gctgcaccgc
    23761 gccattaaat atctgctggc gaaagagcag gggcatcagg gcaacaccac tgaaaccggc
    23821 ggctaccatt attcgatgga agagatgttg caactgggtc agcactgttc gatggcggaa
    23881 cgtcgtgccg acgaagcaac gcgcgatgtc gctgactggc tgaagtgtga cttcatgctc
    23941 gaccaggtag gtaacgtctt taaaggcgta atttccagcg tcactggctt tggcttcttc
    24001 gtccgtctgg acgacttgtt cattgatggt ctggtccatg tctcttcgct ggacaatgac
    24061 tactatcgct ttgaccaggt agggcaacgc ctgatggggg aatccagcgg ccagacttat
    24121 cgcctgggcg atcgcgtgga agttcgcgtc gaagcggtta atatggacga gcgcaaaatc
    24181 gactttagtc tgatctccag tgaacgcgca ccgcgcaacg tcggtaaaac ggcgcgcgag
    24241 aaagcgaaaa aaggcgatgc aggcaaaaaa ggcggcaagc gtcgtcaggt cggtaaaaag
    24301 gtaaactttg agccagacag cgccttccgc ggtgagaaaa aaacgaagcc gaaagcggcg
    24361 aagaaagacg cgagaaaagc gaaaaagcca tcggcgaaaa cgcagaaaat agccgcagcg
    24421 accaaagcga agcgtgcggc gaagaaaaaa gtggcagagt gatcaatacc ctctttaaaa
    24481 aaagagggtt agattgctga caaaatgcgc tttgttcatg ccggatgcgg cgtgaacgcc
    24541 ttatccggcc tacataatca cgcaaattca atatattgca gagatcatgt aggccggata
    24601 aacgtagtgc atcaggcaaa acgtaaacaa cgagtacatt aatgagcgaa atgatttacg
    24661 gcatccacgc agtgcaggcc ctgctggagc gcgcccctga acgttttcag gaagtcttta
    24721 ttttaaaagg ccgtgaagat aaacgtctgt taccgctgat tcacgccctt gagtctcagg
    24781 gcgtggttat ccagttggca aaccgccaat atctcgacga gaaaagcgac ggtgccgtgc
    24841 atcagggcat tatcgcccgc gtgaagccag gacgtcagta tcaggaaaac gatctgccgg
    24901 atctgatcgc ttcgctcgat caaccgttcc tgctgatcct cgacggcgta accgatccgc
    24961 acaacctcgg cgcgtgcctg cgtagtgcgg acgccgcagg cgttcatgcg gtgattgtgc
    25021 cgaaagatcg ctccgcacag ctcaacgcta cggcgaaaaa agtagcctgc ggcgcggcag
    25081 aaagcgttcc gctgattcgg gtgactaacc ttgcgcgcac catgcgtatg ttgcaggaag
    25141 agaatatctg gatcgtcggt acggcaggcg aggcggatca tacactctat cagagcaaaa
    25201 tgaccggacg cctggcgctg gtgatgggcg cggaaggtga aggtatgcgt cgcctgaccc
    25261 gtgaacattg cgatgagttg atcagcatcc caatggcagg aagtgtttct tccctgaacg
    25321 tttcggttgc aaccggaatt tgcttatttg aagcggttcg tcagcgcagc tagttcctct
    25381 gaattatgaa aagccatcca gatttggatg gtttttt
//
LOCUS       DAEEUE010000054        24955 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.054, whole genome shotgun sequence.
ACCESSION   DAEEUE010000054 DAEEUE010000000
VERSION     DAEEUE010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24955
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.054"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(50..274)
                     /gene="cspD"
                     /locus_tag="KLK84_002378"
     CDS             complement(50..274)
                     /gene="cspD"
                     /locus_tag="KLK84_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HBE2009673.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            597..917
                     /gene="clpS"
                     /locus_tag="KLK84_002379"
     CDS             597..917
                     /gene="clpS"
                     /locus_tag="KLK84_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HBE2009674.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            948..3224
                     /gene="clpA"
                     /locus_tag="KLK84_002380"
     CDS             948..3224
                     /gene="clpA"
                     /locus_tag="KLK84_002380"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HBE2009675.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(3627..3714)
                     /locus_tag="KLK84_002381"
     tRNA            complement(3627..3714)
                     /locus_tag="KLK84_002381"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3678..3680),aa:Ser,seq:gga)
     gene            complement(3968..4186)
                     /gene="infA"
                     /locus_tag="KLK84_002382"
     CDS             complement(3968..4186)
                     /gene="infA"
                     /locus_tag="KLK84_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBE2009676.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(4471..5175)
                     /gene="aat"
                     /locus_tag="KLK84_002383"
     CDS             complement(4471..5175)
                     /gene="aat"
                     /locus_tag="KLK84_002383"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HBE2009677.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(5217..6938)
                     /gene="cydC"
                     /locus_tag="KLK84_002384"
     CDS             complement(5217..6938)
                     /gene="cydC"
                     /locus_tag="KLK84_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HBE2009678.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(6939..8705)
                     /gene="cydD"
                     /locus_tag="KLK84_002385"
     CDS             complement(6939..8705)
                     /gene="cydD"
                     /locus_tag="KLK84_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HBE2009679.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(8828..9793)
                     /gene="trxB"
                     /locus_tag="KLK84_002386"
     CDS             complement(8828..9793)
                     /gene="trxB"
                     /locus_tag="KLK84_002386"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBE2009680.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNA
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            10337..10831
                     /gene="lrp"
                     /locus_tag="KLK84_002387"
     CDS             10337..10831
                     /gene="lrp"
                     /locus_tag="KLK84_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HBE2009681.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            10966..14994
                     /gene="ftsK"
                     /locus_tag="KLK84_002388"
     CDS             10966..14994
                     /gene="ftsK"
                     /locus_tag="KLK84_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBE2009682.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANGGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFVQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            15149..15760
                     /gene="lolA"
                     /locus_tag="KLK84_002389"
     CDS             15149..15760
                     /gene="lolA"
                     /locus_tag="KLK84_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HBE2009683.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            15771..17114
                     /gene="rarA"
                     /locus_tag="KLK84_002390"
                     /gene_synonym="mgsA"
     CDS             15771..17114
                     /gene="rarA"
                     /locus_tag="KLK84_002390"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HBE2009684.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLIEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            17205..18497
                     /gene="serS"
                     /locus_tag="KLK84_002391"
     CDS             17205..18497
                     /gene="serS"
                     /locus_tag="KLK84_002391"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBE2009685.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMIAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            18737..21181
                     /gene="dmsA"
                     /locus_tag="KLK84_002392"
                     /pseudo
     CDS             18737..21181
                     /gene="dmsA"
                     /locus_tag="KLK84_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            21192..21809
                     /gene="dmsB"
                     /locus_tag="KLK84_002393"
     CDS             21192..21809
                     /gene="dmsB"
                     /locus_tag="KLK84_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBE2009686.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            21811..22602
                     /locus_tag="KLK84_002394"
     CDS             21811..22602
                     /locus_tag="KLK84_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HBE2009687.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(22638..23264)
                     /locus_tag="KLK84_002395"
     CDS             complement(22638..23264)
                     /locus_tag="KLK84_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBE2009688.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            23579..24727
                     /locus_tag="KLK84_002396"
     CDS             23579..24727
                     /locus_tag="KLK84_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE2009689.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         6222 a         6161 c         6644 g         5928 t
ORIGIN      
        1 gatcgggctg gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg
       61 ccgcttctac ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga
      121 catcaaactg aacggattgt ccagctttta gcgttctgta accatccatc tgaatggtgg
      181 aataatgagc gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaccctttgg
      241 cattgttgaa ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat
      301 acaagtaaga tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct
      361 agaaatacaa tttagagaat tagggagagc cgtcaagcat ttgacagggg ccagggggca
      421 ggtatgaatc aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct
      481 gatgccaacg atagatgata gttatctatc atgtggagta gattggtcag gcaaataagc
      541 tcttgtcagc ggcagggcgt tctgccgata accgtaaccg aagatgataa ctgacaatgg
      601 gtaaaacgaa cgactggctg gactttgatc aactggcgga agaaaaagtt cgcgacgcgc
      661 taaaaccgcc atctatgtat aaagtgatat tagtcaatga tgattacact ccgatggagt
      721 ttgttattga cgtgttacaa aaattctttt cttatgatgt agaacgtgca acgcaattga
      781 tgctcgctgt tcactaccag gggaaggcca tttgcggagt ctttaccgcc gaggttgcag
      841 aaaccaaagt ggcgatggtg aacaagtacg cgagggagaa tgagcatcca ttgctgtgta
      901 cgctagaaaa agcctgaatg caggcataaa aattggggga ggtgcctatg ctcaatcaag
      961 aactggaact cagtttaaat atggctttcg ccagagcgcg cgagcaccgt catgagttta
     1021 tgaccgtcga gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg gaggcgctgg
     1081 aagcgtgttc tgtggatttg gttgcgctcc gtcaggaact ggaagccttt attgaacaaa
     1141 ccacacccgt tctgcctgcc agtgaagagg agcgcgacac acagccgacg ctgagttttc
     1201 agcgtgtact gcaacgtgcg gtcttccatg tccagtcctc cggtcgcaat gaggttaccg
     1261 gtgcaaacgt tctggtcgct atcttcagcg aacaggagtc gcaggcggca tatctgttgc
     1321 gtaaacatga agtcagccgt ctcgatgtgg tgaactttat ctctcatggc acgcgtaaag
     1381 acgagccgac acagtcttct gatcctggca gccagccaaa cagcgaagaa caagctggtg
     1441 gggaggaacg tatggagaat ttcacgacga acctgaacca gcttgcgcgt gtgggcggaa
     1501 tcgacccact aattggtcgt gagaaagagc tggagcgtgc tattcaggtt ctctgccgtc
     1561 gccgtaaaaa caacccgctg ctggtggggg aatctggtgt cggtaaaacc gcgattgcag
     1621 aaggtcttgc ctggcgaatt gttcagggtg atgtgccgga agtgatggct gactgtacga
     1681 tttactctct cgatatcggt tctctgttag cgggcacaaa atatcgcggc gactttgaaa
     1741 aacgttttaa agcgttgctc aagcagctgg agcaggacac taacagcatc ctgtttattg
     1801 atgagatcca caccattatc ggtgcgggtg cagcgtctgg tggtcaggtc gatgcggcta
     1861 acctaatcaa accgttgctc tccagcggta aaattcgcgt aattggttcg acaacctatc
     1921 aggagttcag caacattttc gagaaagacc gtgctctggc gcgtcgcttc cagaaaattg
     1981 atattactga accgtcgatc gaagaaactg ttcaaatcat caatggcctg aaaccgaagt
     2041 atgaagcgca ccacgacgtg cgttataccg caaaagcggt gcgtgcggcg gtagagctgg
     2101 cggtgaaata cattaacgat cgtcatctgc cggataaagc cattgatgtt atcgacgaag
     2161 cgggcgctcg cgcacgtctg atgccggtaa gcaaacgcaa gaaaaccgtt aatgtggcgg
     2221 atattgagtc cgtggtggcc cgtattgcac gcattccaga gaagagtgtt tctcagagtg
     2281 accgcgatac cctgaaaaaa ctcagcgatc gcctgaaaat gctggtcttc ggccaggata
     2341 aagccattga ggcgctgact gaagccatta agatggcgcg tgcaggttta ggtcacgaac
     2401 ataaaccggt cggttcgttc ctgtttgccg gtcctaccgg ggtcgggaaa acagaggtga
     2461 cggtacagct ttcgaaagcg ttgggcattg agcttctgcg ctttgatatg tccgagtata
     2521 tggaacgcca taccgtcagc cgtctgattg gtgcgcctcc gggatacgtt ggttttgatc
     2581 agggcggttt gctgaccgat gcggtaatca agcatccaca tgcggtgctg ctgctggacg
     2641 aaatcgagaa agcgcatccg gacgtgttca atattctgtt gcaggtgatg gacaacggta
     2701 cgctgaccga taacaacgga cgcaaagcgg acttccgtaa cgtggtgctg gtgatgacca
     2761 ctaacgccgg ggtacgggaa actgagcgca aatccattgg tcttatccac caggataaca
     2821 gcaccgatgc gatggaagag atcaagaaga tctttacacc ggaattccgt aaccgtctcg
     2881 acaacattat ctggttcgat catctgtcaa ctgacgtgat ccatcaggtg gtggataaat
     2941 tcatcgtcga gttgcaggtt cagctggatc agaaaggtgt ttctctggaa gtgagccagg
     3001 aagcgcgtaa ctggctggcc gagaaaggtt acgaccgggc aatgggcgca cgtccgatgg
     3061 cgcgtgtcat ccaggacaac ctgaaaaaac cgctcgccaa cgaactgctg tttggttcgc
     3121 tggtggacgg cggtcaggtg acggttgcgc tggataaaga gaaaaatgag ctgacttacg
     3181 gattccagag tgcacaaaag cacaaggcgg aagcagcgca ttaatctgat tgtcaggtag
     3241 gttggtgaag tccgtaatct cgtcaggggt tacggacttt ttatttatgg ggtggaacgg
     3301 tgaaaaccct atttttggag gtgaaggtaa gttgttgata attagtgctg ctggaaggta
     3361 aggataaaaa agggtgctgc aggagaatgg gatggttttg ctttattaac aacgggctaa
     3421 acgtgtagta tttgagttca ctgccgtaca ggcagcttag aaatcgacgg ggtgcggtaa
     3481 aacctttgcg aacgcgttca ctgccgtaca ggcagcttag aaattcacag gtaacatact
     3541 ccacccgccc accatgttca ctgccgtaca gacagataaa atgcgaaaaa aaagctcgca
     3601 ctttcgtacg agctcttctt taaatatggc ggtgaggggg ggattcgaac ccccgatacg
     3661 ttgccgtata cacactttcc aggcgtgctc cttcagccac tcggacacct caccaaattg
     3721 ttttgctacc aaacctcatg ggtggcaacg gggcgctact atagggagtt ggagtaaaac
     3781 ggtcaagaag aattttaatg ataattattg tttgctcata ctgtaaacaa cttgtgcagt
     3841 atatctacat cgagacaagt tatggactta tacttccaaa gtacttcata catatcacaa
     3901 aataaaaagg ccggttaaac cgacctttta ctcgttcttt ctcttcgccc atcaggcggt
     3961 aaaacaatca gcgactacgg aagacaatgc ggcctttgct caggtcgtac ggggtcagtt
     4021 caacagtcac tttgtcgccc gtcaggatgc ggatgtagtt tttgcgcatt ttaccggaga
     4081 tgtgtgcagt aaccacgtga ccgttttcta actctacgcg gaacatggta ttaggcaacg
     4141 tttcaagaac ggtaccttgc atttcaatat tgtcttcttt ggccatctaa tcctctgggg
     4201 tatcactacc gtaatttgaa ccggcaagat aatgccgaag ttctgtaaat aagtaaagat
     4261 ttgcgcgcta aatcgcaaca aacaggttcg gcacattact ccgaaaacac acggctaagc
     4321 cgcaccaaaa gcgcaacgta taagggagcg gtgagataaa cgatgggcgt tacctgacgc
     4381 gaaaaattcc ttatcggcag cggggtaatg agcgtaacca actctgcgac cgcaattata
     4441 acactctggg gagaaatgtg ccgaaaacat tcattcttgt ggtgaaaaca agcaccgtgg
     4501 tacccagaaa ttattcggca atcgtccgag gcgcatttga ttgagataat taaggtaatc
     4561 ccggcgggga atttcgcagg caccaagcga tgctgtgtga tcgttaagga cctggcagtc
     4621 gataagctta ccgccatgac cgataaattc ctcacagaat accagaagcg ccgttttaga
     4681 cgcattttcc atccggctga acatggactc gccacaaaat agcgttccct gggccacgcc
     4741 gtacataccg ccgacaagct catcttcacg ccagacttca atggagtggg catgaccgag
     4801 ttcgtgaagg cgatggtagg cttcgaccac gccacgcgtg atccaggttc cttcttcgcg
     4861 atcgctggca cagccttcaa tgacctgacc aaaagcgtaa ttcatcgtga cacgataggg
     4921 cgagcgttta tgaaatcgct tcatactacg gctgatatgc agtgattctg gccatagcac
     4981 cgcgcgggga tcgggcgacc accagaggat ggggtcgcct ggagaaaacc acggaaaaat
     5041 accacgctgg taagccatta acaggcgcgc agggctaaga tcgcccccaa gtgccagcag
     5101 gccgttaggc tcacgtaatg cgccttccgg ggaagggaag gctattgaat ggcgagaaag
     5161 ctgaaccaag cgcatgaccg caaaactcca cgcaagtcgg atcgttcaat aatagcttac
     5221 aaaccctgct tgaactggta ataacgcccc tgtctggcaa gcagttctgc gtgagtacct
     5281 tgctcaataa tttgcccgtt gtccatcact attatttgtt ggaaacgaga gagtccgcga
     5341 agtcgatggg tgaccattaa caccgttttc tcacgcatca tttttgcaag caattcaagg
     5401 atctggcttt cggttgtggc atctaagcct tcggtaggtt catccagcaa caccagtggc
     5461 gcatcatgta acagcgtacg ggcgatagcc agacggcgca gttcaccacc ggagagctgg
     5521 cgtccgcctt cacctaacca actgttgaga cctgcatcct cgagcagctt ctccagacca
     5581 acgcgacgca agatctccga cagagcctca tcactactgc caggcgaggc gagtaacaga
     5641 ttatcacgca gcgtggcgct aaacagatgc actcgctgag gaacaacgct gatggtctgt
     5701 cgtagagccg cttcattcag gctggctatg gggctatcgt taagcaaaat ctcgccctgt
     5761 tgcgggtccc atgcgcgggt cagcagttgt aacagtgttg atttgccgca tccggttcgc
     5821 ccgagaatcg ctatatgttc cccggcgttt acctgaagag aaatcccttt aagtgcctgt
     5881 tgggattgct ccggataagt gaactgaaca tcccgtaatg tcagcgaaac gcgatcggca
     5941 acacgagttt gggtatcagg gaaggtgacc tccggttttt gatccgttaa gtcagagata
     6001 cgtacggcag aggcaatgac ttgccccaga tgctgaaatg cacccgttac tggtgccagt
     6061 gcttcaaacg cggctaacgc acaaaagaca aacagggcaa ttaatgcgcc gggttgagca
     6121 ttgccgccaa cgccgccaga cgccatccac agcatcagga tcaccgctaa cgcgccaatg
     6181 agcagcatta tcgcttgcga caatgcggtc agttcagatt gacggcgttg cgcttccagc
     6241 cattgaattt ctgtattctc tagttgcgtg cgataacgat cgctggcacc aaaaatggtc
     6301 agctcagctt gcccttgcag ccaggccgtc agttgttggc gatactgtcc gcgaagatga
     6361 gtcagatttt gcccggtgct ttttcccgca cgataaaaca gcggtggcat caggaaaagc
     6421 gtcagtaaca taacgctgcc cagcgtaaag gcgagggtga aatcaaggaa acttaacccg
     6481 attgtcacca ccataatcac cacaaaagcg cccaccagcg gcgagataac gcgcaggtaa
     6541 agatgatcga gcgtatcaac atccgccacc acgcggttga gcaattcgcc ctgacgatag
     6601 cgcgccagtc cggcagggga gaggggcaac aatttgctga aggtgtaaat gcgcagatgc
     6661 tgcaacacgc ggaaagtcgc gtcgtgactt accagacgtt caaaataacg cccggcagta
     6721 cgggtgattg cggcaccacg cacgcccgca gcgggtagca tatagttgaa gctgtacagt
     6781 ccggcaaccc ccgcaaccgc tgaggccgag aggaaccagc cggaaagtgt caacagaccg
     6841 atactggcga gcagcgtcac aattgccagc acaataccaa gacttaacat ccatttatga
     6901 cgtttataca gtgccagata gggtagcaaa gcgcgcattt aaatctcctc ctgacgatgg
     6961 gccagtaatg tggcgaatgg gccaccagcc acacttaatt ccgcgtaacg tccttgctca
     7021 ataatccggc catcctgcat aacccaaatg acatcccagt cagcaagatc ttctaactgg
     7081 tgggtgacca ttaacgttgt ctggcgcaga gaggcggcat tcagcgcctc cattacgcgc
     7141 tgttcactgt gagcatcaag gctggcagcg ggttcatcca acagtaatag cgaacaggga
     7201 tttagtaacg cacgggccac cgccacgcgc tgcgcctgcc ccacggaaag gcgggcagcc
     7261 tggtcgccaa caggtgtatc aacgccttgt ggcaggagcg gtagaaactc gctgacccag
     7321 gcgttatcca gcgctgcttg taattcttgt tcgctggcat caggtcgcgc cagtagtacg
     7381 ttatcccgca attttgctgc cggtaattgt gggttttgcc caacccagga gagatgttta
     7441 cgccatgatt ctggtgataa atcacgtaat tctatcccgt tgattcgtaa tgatccctga
     7501 tatgagagaa aaccagaaag cgcgttcagc agtgagcttt tacctgaacc gctgcggcca
     7561 accaacactg cacgttggcc tgctggcaaa gtaaagttca gcggtccggc cagcgtttta
     7621 ccttccggcg acgtgataaa cagatcctcg gcctcaatgg tcagcggatc ggtcaatgct
     7681 aattccgcct caccgcgctg cggatgggcg agcggggttt ccataaacgt tttcagactg
     7741 tcagctgcac caacagcctg ggctttagca tgataaaacg taccgagatc gcgtaatggc
     7801 tggaaaaact ctggcgcaag gatcagggcc agaaaacctg cagccagcgt caccccagta
     7861 tcgtagtgac caaaatccag ctcgccgaga taggaaaaac caaagtagac cgccaccaga
     7921 gcaattgaca gcgaggtaaa aaattcgaga atgccggagg ataaaaacgc cagtcgtagc
     7981 acttccattg tccgttggcg gaaatcttcc gaagcagaac gaatactttc aatttcagct
     8041 tcaccacgac caaaaatacg caatgtttcc atgccgcgca ggcgatcgag gaaatgccca
     8101 cttaagcgag caagagcgag aaagttacgc cggttagcat cggcagcccc cattccaacc
     8161 agcgccataa acaacggaat tagcggtgct gtgcccagca gaatgagcgc cgcagcccag
     8221 ttagagggga agatcgccac cacaatcagc aacggcaccg acactgccag cgccatttgc
     8281 ggcagatagc gtgcatagta atcatgcata tcgtcaattt gctcgagtac cagcgtcgcc
     8341 cagctccccg caggtttacc ctgaatccac gctggccctg cttgttgcag acggtcgaga
     8401 acctgacggc ggatggcaaa gcggatatgc tgcccggcgt gataacccac ccgttcgcgt
     8461 aaccagacca cccatgcgcg caatacaaag gtcagaacca gtaacgtaaa gggaagcagc
     8521 agggcttcac ggggaatatt ctccataatc atatgttgca gaattcgcgc catgaaccag
     8581 gcctgggcaa tgatcaatat gccgcttaca aagcccagca gacgagaaat attcagccaa
     8641 cgttgggaga tgacgctttg ctgttttaac cagcgggtta actctttttg acgagattta
     8701 ttcattgcac gcttagcagg tgagttatca gaattatttg cagagcaatg ttacaacggg
     8761 gaaaaaataa aggcgaccca tagtcgcaag gtgtcgcctt ctttactttt gttactgatt
     8821 tgtaaaatta ttttgcgtca gctaaaccat cgaggtagcg ttccgcatca agtgctgcca
     8881 tgcagccagt accggccgaa gtaatggcct ggcgataaat gtgatccatc acgtcgcctg
     8941 cggcaaagac gccaggaatg ctggtctggg tggcattacc atgaataccc gactgtactt
     9001 tgatgtagcc gttttccagt tccagctgcc cttcgaaaat cgcagtattc gggctgtgac
     9061 cgatagcaac aaacagaccg gcaacgtcga gtgactcgat gttatcgctg ttttgcgtat
     9121 cgcgcagacg aacgccagtg acacccattt gatcgccggt cacttcttcc agcgtacggt
     9181 tggtgtgcag aatgatgttg ccgttctcca ctttatccat cagacgctta ataaggattt
     9241 tttccgcgcg gaaaccgtca cggcggtgaa tcagatgcac ttccgaagcg atgttagaca
     9301 gatacagcgc ctcttcaacc gcggtattgc cgccgccgat gaccgcaact ttctggttgc
     9361 gatagaagaa accgtcgcag gttgcacaag cagaaacccc acggccttta aaggcttctt
     9421 cagagggcag gccgagatag cgtgcagaag ctccggtggc aataatcagc gcgtcgcaag
     9481 tgtattcggc gttatcgcca ttcagacgga acggacggtt ttgcagatcc accttgttga
     9541 tatgatcaaa aatgatctca gtttcaaact tggtggcatg ttcgtgcatg cgcgccatta
     9601 ataacggacc ggtcagatcg tttggatcac caggccagtt ttccacttcc gtggtggtgg
     9661 tcagttggcc gcctttttcc atgccggtaa tcagcacagg ttgcaggttg gcgcgcgccg
     9721 cgtagacagc agcggtgtat cccgccgggc ctgaacccag gataagcagt ttactgtgtt
     9781 tggtcgtgcc catgagatcc ccatagttgt tggcagacaa tgggcaggat tgtagggaat
     9841 ttacagacgt aaaaaaagag tatgacgatt ttgttaacaa tttgtgcaat cggcagcatc
     9901 gataagcagg tcaaattctc ccgtcattat cacctctgct acttaaattt cccgctttat
     9961 aagccgatta aatgatgaat aaacgccctt gttaatgaat atctggcatg ttgtactaaa
    10021 aatcgatgtt ttgctttgac aatcccctag tgttttgcga aaacattcga ggaagaaaaa
    10081 aacagtattc ttatatgcgc ataaccatgt atgtaaatac catgtttacc gtgctagtga
    10141 aatctacgta tggcgtggac agacgccatt cgtgatgtcg atagctgcca caaggcaacg
    10201 gtcttctcac cgtagaccca ggcattgcgc gccgtgaatc ttcatgattt cggtctatcg
    10261 tgacgggtag cgactctgaa cagtgatgtt tcagggtcag acaggagtag ggaaggaata
    10321 cagagagaca ataataatgg tagatagcaa gaagcgccct ggcaaagatc tcgaccgtat
    10381 cgatcgtaac attcttaatg agttgcaaaa ggatgggcgt atttctaacg tcgagctttc
    10441 taaacgtgtg ggactttccc caacgccgtg ccttgagcgt gtgcgtcggc tggaaagaca
    10501 agggtttatt cagggctata cggcgctgct taacccccat tatctggatg catcacttct
    10561 ggtattcgtt gagattactc tgaatcgtgg cgcaccggat gtgtttgaac aattcaatac
    10621 cgctgtacaa aaacttgaag aaattcagga gtgtcattta gtatctggtg atttcgacta
    10681 cctgttgaaa acacgcgtgc cggatatgtc agcttaccgt aagttgctgg gggaaaccct
    10741 gctgcgtctg cctggcgtca atgacacacg gacatacgtt gttatggaag aagtcaagca
    10801 gagtaatcgt ctggttatta agacgcgcta acacggaaca ggtgcaaaat cggcgtattt
    10861 tgattacact cctgttaatc catacagcaa cagtactggg gtaacctggt actgttgtcc
    10921 gttttagcat cgggcaggaa aagcctgtaa cctggagagc ctttcttgag ccaggaatac
    10981 actgaagaca aagaagtcac attgacaaag ttaagtagcg gccgccgcct tctggaggca
    11041 ttgctgatcc ttattgtcct gtttgccgtc tggttgatgg ctgccttact aagctttaac
    11101 ccttcggacc ccagctggtc gcaaacggcc tggcatgaac ctatccataa tttaggtggg
    11161 atgcccggtg cgtggttggc agatacgctg ttctttattt ttggcgtgat ggcttacacc
    11221 attcccgtca ttattgtcgg cggttgttgg tttgcctggc gtcatcagtc cagcgacgaa
    11281 tacattgatt attttgccgt ttcgctacgc atcattggcg ttttggcgct catccttacc
    11341 tcctgtggtc tggcggcaat caacgctgac gatatctggt attttgcctc cggtggcgtc
    11401 attggcagct tactaagcac tacgctacaa ccactgctac acagtagcgg gggaactatt
    11461 gcgctgctct gcgtttgggc agcgggcctg acgttgttca ccggttggtc atgggtgacc
    11521 attgctgaaa aactcggcgg ctggatttta aacattctca ccttcgccag taatcgtacc
    11581 cgtcgcgatg atacctgggt cgatgaagat gagtatgaag acgacgaaga gtatgaagat
    11641 gaaaatcacg gcaaacagca tgaatcacgc cgtgcccgta ttcttcgcgg cgcgctagcg
    11701 cgtcgtaaac ggttggcgga aaaattcatt aatccgatgg ggcggcaaac agacgctgcg
    11761 ttgttctccg gtaagcggat ggatgatgac gaagagatta cctacactgc acgcggtgtg
    11821 gctgccgacc cggacgacgt cctattttcg ggcaatcgtg caacgcagcc agaatatgac
    11881 gaatacgatc cattattaaa cggtgcgcca attaccgaac ctgtcgctgt agcagctgct
    11941 gctaccacgg cgacacaaag ctgggctgcg ccggttgaac ctgtgactca gacgccgcct
    12001 gttgcctctg ttgatgttcc accttcgcaa cctacagtag cctggcagcc tgtaccgggt
    12061 ccacaaacgg gagagccggt tattgctcct gcaccggaag gttacccaca gcagtcacaa
    12121 tatgcgcagc ctgcagtgca atataatgag ccgctgcaac aaccagtaca gccgcagcag
    12181 ccgtattatg cacctgcagc tgaacaacct gcgcaacagc cgtattatgc ccctgcgcca
    12241 gaacaaccgg tggcaggtaa cgcctggcaa gccgaagagc agcaatccac ttttgctcca
    12301 cagtctacat accagactga gcaaacttat cagcagccag ccgctcagga gccgttgtac
    12361 caacagccgc aacccgttga acagcagcct gttgtggagc ctgaacccgt tgtagaagag
    12421 acaaaacccg cgcgtccgcc gctttactat tttgaagaag tggaagagaa gcgagcccgt
    12481 gaacgtgaac aacttgcggc ctggtatcaa ccgattccag aaccggttaa agaaccagaa
    12541 ccgatcaaat cttcgctgaa agcaccttct gttgcagcag tacctccagt agaagccgct
    12601 gccgctgttt ctccgctggc atctggcgtg aaaaaagcga cactggcgac gggggctgcc
    12661 gcaaccgttg ccgcgccagt cttcagtctg gcaaatggcg gtggaccgcg tccgcaggtc
    12721 aaagagggga ttggcccgca gttgccacga ccgaaacgta tccgcgtgcc aactcgtcgt
    12781 gaattggcgt cttacggtat taagctgccc tcacagcgtg cggcggaaga aaaagcccgt
    12841 gaagcccagc gcaatcagta tgattctggc gatcagtata acgatgatga aatcgatgcg
    12901 atgcagcagg atgaactggc acgtcagttc gcccagacac agcagcaacg ctatggcgaa
    12961 cagtatcaac atgatgtgcc cgtaaacgca gaagatgcag atgctgcggc agaggctgaa
    13021 ctggctcgtc agtttgtgca aactcaacaa caacgttatt ccggcgaaca accggctggg
    13081 gcgaatccgt tctcgctgga tgattttgaa ttttcgccaa tgaaagcgtt gctggatgat
    13141 ggtccacacg aaccgctgtt tacgccaatt gttgaacctg tacagcagcc gcaacaaccg
    13201 gttgcaccac agcagcagta tcagcagcca caacagccgg ttgcgccaca gccgcagtat
    13261 cagcagccac aacagcaggt tgcgccgcag ccacaatatc agcagccgca acaaccggtt
    13321 gcgccacagc agcaatatca gcagccgcaa caaccagttg cgccgcagcc gcagtatcaa
    13381 cagccgcaac aaccggttgc accgcagccg cagtatcagc agccacaaca gccagttgcg
    13441 ccacagccgc aggataccct gcttcatccg ctgttgatgc gtaatggcga cagccgtccg
    13501 ttgcacaaac cgacgacgcc gctgccttct ctggatttgc tgacaccgcc gccgagcgaa
    13561 gtggagccgg tagatacctt tgcgcttgaa caaatggcgc gtctggtgga agcgcgtctg
    13621 gctgatttcc gtattaaagc cgatgtcgtt aattactctc cggggccagt tatcactcgc
    13681 tttgaactta acctggctcc tggcgtaaaa gcggcgcgca tttctaactt gtcacgggac
    13741 cttgcccgtt cactttcgac ggtggcggtg cgtgtcgttg aagttattcc tggcaaaccc
    13801 tatgtaggtc tggagttacc aaataaaaaa cgacaaaccg tttatctgcg cgaagttttg
    13861 gataacgcca aattccgcga taatccgtcg ccattaaccg tggtgctggg taaagatatc
    13921 gccggtgagc cggtggttgc cgatctggcg aaaatgccgc acttgttggt tgcggggact
    13981 accggttccg gtaaatctgt cggtgtgaac gcgatgatcc tgagcatgct ttataaagca
    14041 cagccagaag atgtgcgttt catcatgatc gacccgaaaa tgctggagct ttcggtttat
    14101 gaaggcattc cgcacctgtt aacggaagtc gttactgata tgaaagatgc cgccaacgcg
    14161 ctgcgctggt gtgttaacga gatggagcgt cggtataaac tgatgtctgc gctgggtgtg
    14221 cgtaatctgg cgggttataa cgaaaaaatt gctgaagccg atcgcatgat gcgtccgatt
    14281 ccagacccgt actggaagcc gggtgacagt atggatgccc agcatccggt gctgaaaaaa
    14341 gaaccataca ttgtcgtgtt ggttgacgaa tttgccgacc tgatgatgac ggtaggtaaa
    14401 aaagtggaag agctgatagc tcgtctggcg caaaaagccc gtgccgcggg tatccacctc
    14461 gtactggcaa ctcagcgtcc atcggttgat gttattactg gtctgattaa agcgaatatt
    14521 ccgacccgta tcgcctttac cgtatccagt aagattgact cacgtaccat tcttgatcag
    14581 gctggcgcgg aatcactgct gggtatgggg gatatgctct actctgggcc gaactccacg
    14641 ttgccggtac gtgtccatgg tgcttttgtt cgcgatcagg aagttcatgc cgtggtgcag
    14701 gactggaaag cgcgtggtcg cccacagtat gttgatggca tcacctccga cagcgaaagc
    14761 gaaggtggtg cgggtggttt cgatggcgct gaagaactgg atccgttgtt cgatcaggcg
    14821 gtgcagtttg tcactgaaaa acgcaaagcg tcaatttctg gcgtacagcg tcagttccgc
    14881 attggttata accgtgcagc gcgtattatc gaacagatgg aagcgcaggg gattgtcagc
    14941 gaacaggggc acaacggtaa tcgtgaagtg ctggccccac cgccgtttga ctaattaatg
    15001 cattgccgga taaggcgcgg tagcgtcgca tccggcactc tatcaactga aaattcagta
    15061 ttttcttctt tcctcaagct gattattagc ctggaataga gagtagaggg aactcccgat
    15121 cgggagtgac gtaatttgag gaataatgat gaaaaaaatt gctatcacct gtgcattact
    15181 ctcaagctta gtagcaagca gcgtttgggc tgatgccgca agcgatctga aaagccgcct
    15241 ggataaagtc agcagcttcc acgccagctt cacacaaaaa gtgactgacg gtagcggcgc
    15301 ggcggtgcag gaaggtcagg gcgatctgtg ggtgaaacgt ccaaacttat tcaactggca
    15361 tatgacacaa cctgatgaaa gcattctggt ttctgacggt aaaacactgt ggttctataa
    15421 cccgttcgtt gagcaagcta cggcaacctg gctgaaagat gccaccggta atacgccgtt
    15481 tatgctgatt gcccgcaacc agtccagcga ctggcagcag tacaatatca aacagaatgg
    15541 cgatgacttt gtcctgacgc cgaaagccag caatggcaat ctgaagcagt tcaccattaa
    15601 cgtgggacgt gatggcacaa tccatcagtt tagcgcggtg gagcaggacg atcagcgcag
    15661 cagttatcag ctgaaatccc agcaaaatgg ggctgtggat gcagcgaaat ttaccttcac
    15721 cccgccgcaa ggcgtcacgg tagatgatca acgtaagtag aggcacctga gtgagcaatc
    15781 tgtcgctcga tttttcggat aatacttttc aacctctggc cgcgcgtatg cggccagaaa
    15841 atttagcaca gtatatcggt cagcaacatt tgctggctgc ggggaagccg ttgccgcgcg
    15901 ctatcgaagc cgggcatttg cattctatga tcctctgggg gccaccgggt accggcaaaa
    15961 caaccctcgc tgaagtgatt gcccgctatg cgaacgctga tgtggaacgt atttctgccg
    16021 tcacctctgg cgtgaaagag attcgcgagg cgatcgagcg cgcccggcaa aaccgcaatg
    16081 caggtcgccg cactattctt tttgttgacg aagttcaccg tttcaacaaa agccagcagg
    16141 atgcatttct gccacatatt gaagacggca ccatcacttt tattggcgca accactgaaa
    16201 acccgtcgtt tgagcttaat tcggcactgc tttcccgtgc ccgtgtttat ctgttgaaat
    16261 ccctgagtac agaggatatt gagcaagtac taactcaggc gatggaagac aaaacccgtg
    16321 gctatggtgg tcaggatatt gttctgccag atgatacacg acgtgccatt gcagaactgg
    16381 tgaatggcga cgcgcgccgg gcgttaaata cgctggaaat gatggcggat atggccgaag
    16441 tctatgatag cggtaagcgg gtcctgaagc ctgaattact gatcgaaatc gccggtgaac
    16501 gtagcgcccg ctttgataac aaaggcgatc gcttttacga tctaatttcc gcactgcata
    16561 agtcggtacg tggtagcgca cccgatgcgg cgctgtactg gtatgcgcga attattaccg
    16621 ctggtggcga tccgttatat gtcgcgcgtc gctgtctggc gattgcgtct gaagacgtcg
    16681 gtaatgccga tccacgggcg atgcaggtgg caattgcggc ctgggattgc tttactcgcg
    16741 ttggcccggc ggaaggtgaa cgcgccattg ctcaggcgat tgtttacctg gcctgcgcgc
    16801 caaaaagcaa cgctgtctac actgcgttta aagccgcgct ggccgatgct cgcgaacgcc
    16861 cggattatga cgtgccggtt catttgcgta atgcgccgac gaaattaatg aaggaaatgg
    16921 gctacgggca ggaatatcgt tacgctcatg atgaagcaaa cgcttatgct gccggtgagg
    16981 tttacttccc gccggaaata gcacaaacac gctattattt cccgacaaac aggggccttg
    17041 aaggcaagat tggtgaaaag ctcgcctggc tggctgaaca ggatcaaaat agccccataa
    17101 aacgctaccg ttaatgttat cgttgcggta atgttgttac tgtatccctg tggtcgcagg
    17161 ctgtggccac atctcccatt taattcgata agcacaggat aagcatgctc gatcccaatc
    17221 tgctgcgtaa tgagccagac gcagtcgctg aaaaactggc acgccggggc tttaagctgg
    17281 atgtagataa gctgggcgct cttgaagagc gtcgtaaagt attgcaggtc aaaacggaaa
    17341 acctgcaagc ggagcgtaac tcccgatcga aatccattgg ccaggcgaaa gcgcgcgggg
    17401 aagatatcga gcctttacgt ctggaagtga acaaactggg cgaagagctg gatgcagcaa
    17461 aagccgagct ggatgcttta caggctgaaa ttcgcgatat cgcgctgacc atccctaacc
    17521 tgcctgcaga tgaagtgccg gtaggtaaag acgaaaatga caacgttgaa gtcagccgct
    17581 ggggtacccc gcgtgagttt gactttgaag ttcgtgacca cgtgacgctg ggtgaaatgc
    17641 actctggcct cgactttgca gctgcagtta agctgactgg ttcccgcttt gtggtaatga
    17701 aagggcagat tgctcgcatg caccgcgcac tgtcgcagtt tatgctggat ctgcataccg
    17761 aacagcatgg ctacagtgag aactatgttc cgtacctggt taaccaggac acgctgtacg
    17821 gtacgggtca gctgccgaaa tttgctggcg atctgttcca tactcgtccg ctggaagaag
    17881 aagcagacac cagtaactat gcgctgatcc caacggcaga agttccgctg actaacctgg
    17941 tgcgcggtga aatcatcgat gaagatgatc tgccaattaa gatgatcgcc cacaccccat
    18001 gcttccgttc tgaagccggt tcatatggtc gtgacacccg tggtctgatc cgtatgcacc
    18061 agttcgacaa agttgaaatg gtgcagatcg tgcgcccaga agactcaatg gcggcgctgg
    18121 aagagatgac tggtcatgca gaaaaagtcc tgcagttgct gggcctgccg taccgtaaaa
    18181 tcatcctttg cactggcgac atgggctttg gcgcttgcaa aacttacgac ctggaagtat
    18241 ggatcccggc acagaacacc taccgtgaga tctcttcctg ctccaacgtt tgggatttcc
    18301 aggcacgtcg tatgcaggca cgttgccgca gcaagtcgga caagaaaacc cgtctggttc
    18361 ataccctgaa cggttctggt ctggctgttg gtcgtacgct ggttgcagta atggaaaact
    18421 atcagcaggc tgatggtcgt attgaagtac cagaagttct gcgtccgtat atgaacggac
    18481 tggaatatat tggctaatac ccaatttttc tgaatctaaa aagcgccttt agggcgcttt
    18541 ttttgtctcc ctttgatacc gaacaataat tactcctcac ttacacgtaa tactactttc
    18601 gagtgaaaat ctaccgatct ctttgatttt caaattattc tatgtataca agcctatata
    18661 gcgagctgct atagaaataa ttacacaata cggtttgtta ctggaatcaa tcgtgatcaa
    18721 gcttgagtga gccattatga aaacgaaaat ccctgatgcg gtattggctg ctgaggagag
    18781 tcgccgtggt ttggtaaaaa cgacagcgat cggcggcctg gcaatggcca gcagcgcatt
    18841 aacattacct tttagtcgga ttgcgcacgc tgtcgatagc gccattccaa caaaatcaga
    18901 cgaaaaggtt atctggagcg cctgtacagt taactgtggt agtcgctgcc cgctacgtat
    18961 gcacgtcgtg gacggtgaaa tcaaatatgt cgaaacggac aataccggcg atgacaatta
    19021 cgacggccta cactaggttc gcgcctgcct gcgtgggcgt tccatgcgtc accgtgtcta
    19081 caatccggac cgcctgaaat atccgatgaa acgagtcggg gcgcgcggtg aaggcaaatt
    19141 cgagcgcatt agctgggaag aagcctacga catcatcgcg accaatatgc agcgcctgat
    19201 caaagagtac ggcaacgagt ctatctatct gaactatggc accggtacgc tgggcggcac
    19261 catgacccgc tcctggccgc cgggaaatac cctggtcgcg cgcctgatga actgctgcgg
    19321 cggttatctt aaccattacg gtgactactc ttccgcgcaa attgcggaag gtttgaacca
    19381 tacctacggc ggctgggcag atggtaacag cccgtcggat atcgaaaaca gtaaattggt
    19441 agtgctgttt ggtaataacc caggcgaaac gcgaatgagt ggcggtgggg tgacttacta
    19501 tcttgaacag gcacgccaga aatctaatgc tcgcatgatc atcatcgatc cgcgctatac
    19561 cgacaccggt gccgggcgcg aagatgagtg gatccctatt cgtccaggaa cagatgccgc
    19621 actggttaac ggtctggcgt acgtcatgat cactaaaaac ctggtggatc aggcattcct
    19681 cgataaatat tgcgttggct atgacgagaa aaccctgccc gccagtgcgc cgaaaaatgg
    19741 tcattataaa gcttatattc tgggtgaagg gccagatggc gtggctaaaa caccggaatg
    19801 ggcctcgcaa atcaccggtg ttccggcaga caaaatcatc aaactggctc gtgaaatcgg
    19861 cagtaccaaa ccggcgttta tcagccaggg atggggcccg cagcgtcacg ctaacggtga
    19921 aatcgcaacc cgtgctatct cgatgctggc gattctgacc ggtaacgttg gtattaatgg
    19981 agggaacagc ggcgcgcgtg aaggttcata cagcttaccg tttgtccgta tgccgacctt
    20041 ggaaaacccg atccagacca gcatttcgat gtttatgtgg accgatgcca ttgaacgtgg
    20101 cccggaaatg acggcgctgc gtgatggtgt gcgcgggaaa gataagctgg atgtgccgat
    20161 caaaatgatc tggaactatg ccggtaactg cctgattaac cagcattctg aaatcaaccg
    20221 tacccatgaa atcctccagg atgataagaa gtgcgagctg attgtggtta tcgactgcca
    20281 catgacctca tcggcgaaat atgctgacat cctgctgcct gactgcaccg cttccgaaca
    20341 gatggacttt gcactggatg catcctgcgg gaatatgtct tacgtgattt tcaacgatca
    20401 ggtgattaaa ccgcgctttg aatgtaagac catctatgaa atgaccagcg aactggcaaa
    20461 acgtcttggc gttgagcaac agtttactga aggccgtacc taggaagagt ggatgcggca
    20521 tctgtatgcc cagtcgcggg aagcgattcc tgaactgcca acgtttgaag agttccgcaa
    20581 gcaggggatc tttaaaaagc gcgacccaca agggcatcac gttgcttata aagccttccg
    20641 tgaagatccg caggcaaacc cactgactac gccatcgggc aaaattgaga tttattcgca
    20701 ggcgctggct gacattgccg ctacctggga attgccagaa ggcgatgtga tcgatccact
    20761 gccgatctac acgccgggct ttgaaagtta tcaggatccg ctgaacaaac agtatccgct
    20821 gcagcttaca ggtttccact ataaatctcg cgttcactca acttacagca acgttgatgt
    20881 gctgaaagcg gcttgccgtc aggaaatgtg gatcaacccg cttgatgccc aaaaacgcgg
    20941 tatccacaac ggcgataaag tcaggatctt taacgatcgt ggtgaggttc atattgaggc
    21001 gaaagtgacg ccacgaatga tgccgggcgt ggtcgcactg ggtgaaggcg cctggtatga
    21061 cccggatgca aaacgtgtcg ataagggcgg ttgtatcaac gtactgacca ctcaacgtcc
    21121 gtctcctctc gctaagggga atccgtcaca tacaaacctt gttcaggttg aaaaggtgta
    21181 aggagtaacc gatgacaacc cagtatggat tttttattga ttccagccgt tgcaccggtt
    21241 gcaaaacctg cgagctggct tgtaaagact acaaagattt gacgccagaa gtcagtttcc
    21301 gccgcattta tgaatatgct ggcggcgact ggcaggaaga taacggtgtc tggcaccaga
    21361 acgtgtttgc ctactatctg tcgatttcgt gcaaccactg cgaagatccg gcttgtacta
    21421 aagtctgccc gagcggtgcg atgcataaac gcgaagatgg ttttgttgtg gtcgatgaag
    21481 atgtatgcat tggctgccgt tactgtcata tggcttgccc gtatggcgca ccgcaatata
    21541 acgaaacgaa aggccatatg accaaatgcg atggttgtta tgaccgtgtt gcggagggta
    21601 aaaagccgat ctgtgttgaa tcttgcccgc tgcgggcgct ggatttcggc cctatcgacg
    21661 agctgcgtaa aaaacatggc gatctggcgg ccgttgcgcc gttgccgcga gctcacttta
    21721 ccaaaccgaa tattgtgatc aaacccaatg ccaatagccg cccgaccggg gataccactg
    21781 gctatctggc aaacccgaag gaggtgtgag atgggaagtg gatggcatga atggccgctg
    21841 atgatcttca cggtcttcgg gcaatgtgtg gcgggtggtt ttatcgttct ggctttggcg
    21901 ctgctcaaag gcgacctgcg agcagaagcc cagcagcgtg ttatcgcctg catgtttggc
    21961 ttatgggtac tgatgggcat tggctttatc gcctctatgc tccatcttgg ttcaccgatg
    22021 cgcgccttta actcgctcaa ccgggtaggg gcttcagcac tcagtaacga aatcgccagc
    22081 ggttcgatct tttttgctgt aggcggcatc ggctggttgc tggcaatgct gaaaaagctg
    22141 tcaccggcat tgcgtacgct atggctggtg gtgacgatgg ttcttggcgt cgtctttgtc
    22201 tggatgatgg tgcgtgtgta taacagcatt gataccgttc cgacctggta cagcatctgg
    22261 acgccgatgg gctgggcgat gcgtctgctg ccagccattt ctgtactggc actggtggtg
    22321 agtggcgtga tgtcagtgat gcagggcaca gagctggcaa ctattcatag ttctgtgcag
    22381 caggccgcag cgctggtgcc ggactatggt gcgctgatgt cctggcggat cgtgcttttg
    22441 gccgttgccc tgtgcttgtg gattgcacca cagctaaaag gttatcagcc tgcggttccg
    22501 ctactatccg tctcattcat tctgcttctg gcaggggaat taatcggtcg cggcgtattc
    22561 tatggtttgc atatgaccgt ggggatggcc gtcgcaagct aatcagtaac aaccggggtt
    22621 tcggccccgg ttctctttta tttctgcttc gttaacgtgt cataactggt catcaaatta
    22681 cgataatcag gaatatggtt agagaacaac gtcgccagtc cttcaatatc attacgccag
    22741 tcacggtgca gctcgcaagc cacgccgaac catgtcatca attgcgcacc agcttgcgac
    22801 atgcgatccc acgccgaatg ccgggtaatt tcattaaagg tccctgatgc atcagtcacg
    22861 acaaagacat caaacccttc ttcaatggct gaaagcgccg ggaatgccac gcaaacttcg
    22921 gttaccacac cggcaataat taactgtttt ttacctgtcg ctttgactgc ttttacaaaa
    22981 tcttcgttat cccaggcgtt aatattgccc gggcgagcaa tgtaaggtgc atcggggaat
    23041 tgtgctttca gttctagaac taatgggccg ttaggaccgg tttcaaaact ggtggtaaga
    23101 atagttggta agttgaaata cttagccagg tcacccagcg ccagcacgtt atttttaaac
    23161 ttatcgggtt cgatatcccg tacaagggaa agtaaaccag cctggtgatc aacaagcaaa
    23221 acggcagcat catttttatc aagacgaaca tacggtttgg tcatcctctt ctcctttcga
    23281 gagtcagtgg tacgtctgaa atcggtcaga cgcgatgcat tgctctgaaa gcatagacgg
    23341 gaaatatgag tttgctgtga ccatgaaatt tttcgactga acgaagcgta tgaaatttgt
    23401 gttagatcaa taaaaacaat cagatgagat taatcatctc ttcagcatta gcgttatgaa
    23461 tgagtaatat aaatcgataa taccgccgct gcgatatgcg cgtattttca cccattgaca
    23521 atgtttttgg cggtggcatg atgcgcatga aatttgaact tcctcacggt tttaattcat
    23581 gtccacgtat acccggcctg tcatgctttt gctgtctggc ctgcttttgt tgactctggc
    23641 gatcgcggtg ttaaatacac tcgtgccgct ttggctcgcc caggaacaca tgtccacatg
    23701 gcaggtaggc gttgtcagct catcctattt taccggcaac cttgtcggta cattgctgac
    23761 agggtatgtc attaaacgca ttggctttaa ccgcagctat tatctgtcct ccttcatttt
    23821 tgctgctggc tgtgccggcc ttggcctgat gattggtttc tggagctggt tggcgtggcg
    23881 ttttgtcgcg ggcgtcggct gtgccatgat ttgggtggtt gttgagagcg cgctgatgtg
    23941 cagtgggacg tcacgtaacc gtgggcgttt gcttgctgcg tatatgatgg tttattacgt
    24001 gggaacgttt ttaggccagt tactggtcag caaagtctca accgagctga tgagcgtatt
    24061 gccgtgggtt acaggtttga cattggcagg gatcttaccg ctgttgttta cgcgtgtcct
    24121 gaatcagcag gctgaaaacc atgattcgac gtcaattacg gcaatgctaa aactccgtca
    24181 ggcgcggctt ggcgtgaatg gctgcattat ctcaggaatc gttctgggat ctctatatgg
    24241 cctgatgcct ctgtacctca atcacaaagg ggtgagcaat gccagtattg gtttctggat
    24301 ggcggtactg gtcagtgcgg gtatcctcgg acaatggccg attggacgtc tggcggataa
    24361 gtttggtcga ctgctggttt tgcgtgttca ggtctttgtc gtcattctcg gcagtattgc
    24421 gatgcttagc caggcggcga tggccccagc gttattcatc ctcggtgccg ctggctttac
    24481 gctatatccg gtggcgatgg cctgggcttg tgagaaagtt gaacatcatc aactggtggc
    24541 gatgaaccaa gccttactgt tgagctatac cgtgggaagt ctgcttggcc cgtcatttag
    24601 cgctatgcta atgcagaatt tctccgataa tttattgttt atcatgatcg ccagcgtatc
    24661 gtttatctat ttgctgatgc tgcttcgcaa cgccggtcat acgccgaaac ccgttgctca
    24721 cgtgtaaatg aattcaagca aagtgtgaac ttactgtttc acactctgct tttttgtttc
    24781 ttctatctga cttgcttttt tccaaatttt attcgtttaa aaataaaatg tgcagcaggt
    24841 tataattttg catttcgcta tttccgcact tcttatttgc cgcgcataat ccctcgtttt
    24901 acagatgccc ctttaatttt ggcgaaggat ttgtctatgg ctgggaatgt tcagg
//
LOCUS       DAEEUE010000055        24604 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.055, whole genome shotgun sequence.
ACCESSION   DAEEUE010000055 DAEEUE010000000
VERSION     DAEEUE010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24604
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.055"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(1..374)
                     /locus_tag="KLK84_002397"
                     /pseudo
     CDS             complement(1..374)
                     /locus_tag="KLK84_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            898..2826
                     /gene="thrS"
                     /locus_tag="KLK84_002398"
     CDS             898..2826
                     /gene="thrS"
                     /locus_tag="KLK84_002398"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBE2009690.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2830..3372
                     /gene="infC"
                     /locus_tag="KLK84_002399"
     CDS             2830..3372
                     /gene="infC"
                     /locus_tag="KLK84_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBE2009691.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3469..3666
                     /gene="rpmI"
                     /locus_tag="KLK84_002400"
     CDS             3469..3666
                     /gene="rpmI"
                     /locus_tag="KLK84_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBE2009692.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3719..4075
                     /gene="rplT"
                     /locus_tag="KLK84_002401"
     CDS             3719..4075
                     /gene="rplT"
                     /locus_tag="KLK84_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBE2009693.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4192..4316
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4198..4242
                     /gene="pheM"
                     /locus_tag="KLK84_002402"
     CDS             4198..4242
                     /gene="pheM"
                     /locus_tag="KLK84_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HBE2009694.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4525..5508
                     /gene="pheS"
                     /locus_tag="KLK84_002403"
     CDS             4525..5508
                     /gene="pheS"
                     /locus_tag="KLK84_002403"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBE2009695.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5523..7910
                     /gene="pheT"
                     /locus_tag="KLK84_002404"
     CDS             5523..7910
                     /gene="pheT"
                     /locus_tag="KLK84_002404"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBE2009696.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNIGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQHRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7915..8214
                     /gene="ihfA"
                     /locus_tag="KLK84_002405"
     CDS             7915..8214
                     /gene="ihfA"
                     /locus_tag="KLK84_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HBE2009697.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8315..9295
                     /gene="btuC"
                     /locus_tag="KLK84_002406"
     CDS             8315..9295
                     /gene="btuC"
                     /locus_tag="KLK84_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HBE2009698.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9358..9909
                     /gene="btuE"
                     /locus_tag="KLK84_002407"
     CDS             9358..9909
                     /gene="btuE"
                     /locus_tag="KLK84_002407"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HBE2009699.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9909..10658
                     /gene="btuD"
                     /locus_tag="KLK84_002408"
     CDS             9909..10658
                     /gene="btuD"
                     /locus_tag="KLK84_002408"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HBE2009700.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     QMAGITSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10736..11161
                     /locus_tag="KLK84_002409"
     CDS             10736..11161
                     /locus_tag="KLK84_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBE2009701.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRDRFDLHLPRETKQQASIGTQIDKDELLPGDLVFFKTGSGQNGLH
                     VGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11408..12121
                     /gene="ydiV"
                     /locus_tag="KLK84_002410"
     CDS             11408..12121
                     /gene="ydiV"
                     /locus_tag="KLK84_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HBE2009702.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12185..13621
                     /locus_tag="KLK84_002411"
     CDS             12185..13621
                     /locus_tag="KLK84_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HBE2009703.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13625..13816)
                     /gene="hemP"
                     /locus_tag="KLK84_002412"
     CDS             complement(13625..13816)
                     /gene="hemP"
                     /locus_tag="KLK84_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HBE2009704.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13948..14994)
                     /gene="aroH"
                     /locus_tag="KLK84_002413"
     CDS             complement(13948..14994)
                     /gene="aroH"
                     /locus_tag="KLK84_002413"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HBE2009705.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAIEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15151..15984)
                     /gene="ppsR"
                     /locus_tag="KLK84_002414"
     CDS             complement(15151..15984)
                     /gene="ppsR"
                     /locus_tag="KLK84_002414"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HBE2009706.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16316..18694
                     /gene="ppsA"
                     /locus_tag="KLK84_002415"
     CDS             16316..18694
                     /gene="ppsA"
                     /locus_tag="KLK84_002415"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HBE2009707.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18751..20397)
                     /gene="fadK"
                     /locus_tag="KLK84_002416"
     CDS             complement(18751..20397)
                     /gene="fadK"
                     /locus_tag="KLK84_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HBE2009708.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20454..20747)
                     /locus_tag="KLK84_002417"
     CDS             complement(20454..20747)
                     /locus_tag="KLK84_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HBE2009709.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20744..22033)
                     /locus_tag="KLK84_002418"
     CDS             complement(20744..22033)
                     /locus_tag="KLK84_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBE2009710.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22089..23027)
                     /locus_tag="KLK84_002419"
                     /pseudo
     CDS             complement(22089..23027)
                     /locus_tag="KLK84_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
     gene            complement(23047..23811)
                     /locus_tag="KLK84_002420"
     CDS             complement(23047..23811)
                     /locus_tag="KLK84_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HBE2009711.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            24127..>24604
                     /locus_tag="KLK84_002421"
     CDS             24127..>24604
                     /locus_tag="KLK84_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBE2009712.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAI"
BASE COUNT         6097 a         5941 c         6462 g         6104 t
ORIGIN      
        1 ctgttcaata taatccagta tgcttcgcaa atggttagct ttaataggcg tggtttccag
       61 taataaggaa atgatttctt ttttatagtg taatgtaatg agtatatttt ctgcatggat
      121 gggtgaagtg gcaaagttaa tggtaaaaag ggcagtggaa taacaatttt gtgatatttt
      181 cgacagtttg cggtaaattc ctgagtaaca cagtatgaca gcatcgaact taagtcagag
      241 gtatcaagta gggtgatgag ctggcgcgca aatttattaa taggctctga tgaaggtgtt
      301 ggtatgtcgc tatcgatgtg caatctatga ggagacttat aatgagttct gataatgata
      361 tcgttttgac tcataatcta ttcgccttat atatatctgg aggttttgag gatgatgatt
      421 aattattatc gccagaacga aatgtgcacg aaaaattgtt ataagaattg tgtcactttt
      481 atcattgtga tatgaccact tcgtcattaa tctcatgagc gtttaaaata ctaccgccaa
      541 atcacgcaac gaactttgct ggattcagtt tatgctgctg taaatccgct cgcagtaaac
      601 ctttcagacg caccgtgatg ttatcagtgg ttctttaagc gttttgctgg tgtactcact
      661 acaaacgaat tgcgaatcga atcaatgtga aacggaaagg tacaatctcc ctttttttag
      721 tgttaacatc gttcaaccgg gtggaaggtg agaggttaat gtctttcatt tgtgtacctg
      781 attgcgaacc aatttagcat ttgttggcta gatggtttcg caatgaactg ttaataaaca
      841 aatttttctt tgtatgtgat ctttcgtgtg ggtcaccact gcaaataagg atataaaatg
      901 cctgttataa ctcttcctga tggcagccaa cgccattacg accacgctgt aagccccatg
      961 gatgttgcgc tggacattgg tccaggtctg gcgaaagcct gtatcgcagg gcgcgttaat
     1021 ggcgaactgg ttgatgcttg cgatctgatt gaaaacgacg cacaactgtc gatcattacc
     1081 gccaaagacg aagaaggtct ggagatcatt cgtcactcct gtgcgcacct gttagggcat
     1141 gcgattaaac aactttggcc gcataccaaa atggcaatcg gcccggttat tgacaacggt
     1201 ttttattacg acgttgatct tgaccgcacg ttaacccagg aagatgtcga agcactcgag
     1261 aagcggatgc atgagcttgc tgagaaaaac tacgacgtca ttaagaagaa agtcagctgg
     1321 cacgaagcgc gtgaaacttt cgccaaccgt ggggagagct acaaagtttc cattcttgac
     1381 gaaaacatcg cccatgatga caagccaggt ctgtacttcc atgaagaata tgtcgatatg
     1441 tgccgcggtc cgcacgtacc gaacatgcgt ttctgccatc atttcaaact aatgaaaacg
     1501 gcaggggctt actggcgtgg cgacagcaac aacaaaatgt tgcaacgtat ttacggtacg
     1561 gcgtgggcag acaaaaaagc acttaacgct tacctgcagc gcctggaaga agccgcgaaa
     1621 cgcgaccacc gtaaaatcgg taaacagctc gacctgtacc atatgcagga agaagcgccg
     1681 ggtatggtat tctggcacaa cgacggctgg accatcttcc gtgaactgga agtgtttgtt
     1741 cgttctaaac tgaaagagta ccagtatcag gaagttaaag gtccgttcat gatggaccgt
     1801 gtcctgtggg aaaaaaccgg tcactgggac aactacaaag atgcaatgtt caccacatct
     1861 tctgagaacc gtgaatactg cattaagccg atgaactgcc cgggtcacgt acaaattttc
     1921 aaccaggggc tgaagtctta tcgcgatctg ccgctgcgta tggccgagtt tggtagctgc
     1981 caccgtaacg agccgtcagg ttcgctgcat ggcctgatgc gcgtgcgtgg atttacccag
     2041 gatgacgcgc atatcttctg tactgaagaa caaattcgcg atgaagttaa cggatgtatc
     2101 cgtttagtct atgatatgta cagcactttt ggcttcgaga agatcgtcgt caaactctcc
     2161 actcgtcctg aaaaacgtat tggcagcgac gaaatgtggg atcgtgctga ggcggacctg
     2221 gcggttgcgc tggaagaaaa caacatcccg tttgaatatc aactgggtga aggcgctttc
     2281 tacggtccga aaattgaatt taccctgtat gactgcctcg atcgtgcatg gcagtgcggt
     2341 acagtacagc tggacttctc cttgccgtct cgtctgagcg cctcctatgt gggcgaagac
     2401 aacgagcgta aggtaccggt aatgattcac cgcgcaattc tggggtcgat ggagcgtttc
     2461 atcggtatcc tgaccgaaga attcgctggt ttcttcccga cctggcttgc gccagttcag
     2521 gttgttatca tgaatattac cgattcacag tctgaatacg ttaacgaatt gacgcaaaaa
     2581 ctatcaaatg cgggcattcg tgttaaagca gacttgagaa atgagaagat tggctttaaa
     2641 atccgcgagc acactttgcg tcgcgtccca tatatgctgg tctgtggtga taaagaggtg
     2701 gaatcaggca aagttgccgt tcgcacccgc cgtggtaaag acctgggaag catggacgta
     2761 aatgaagtga tcgagaagct gcaacaagag attcgcagcc gcagtcttaa acaattggag
     2821 gaataaggta ttaaaggcgg aaaacgagtt caaacggcgc gccctaaccg tatcaatggc
     2881 gaaattcgcg cccaggaagt tcgcttaaca ggtctggaag gcgagcagct tggtattgtg
     2941 agtctgagag aagctctgga gaaagcagaa gaagccggag tagacttagt cgagatcagc
     3001 cctaacgccg agccgccggt ttgtcgtata atggattacg gcaaattcct ctatgaaaag
     3061 agcaagtctt ctaaggaaca gaagaaaaag caaaaagtta tccaggttaa ggaaattaaa
     3121 ttccgtccta gtacagatga aggcgactat caggtaaaac tccgcagcct gattcgcttt
     3181 ctcgaagagg gtgataaagc caaaatcacg ctgcgtttcc gcggtcgtga gatggcgcac
     3241 cagcaaatcg gtatggaagt gcttaatcgc gtgaaagacg atttgcaaga actggcggta
     3301 gtcgaatcct tcccaacgaa gatcgaaggc cgccagatga tcatggtgct cgctcctaag
     3361 aagaaacagt aaggccttca agtagcacta tctgtggagc ctttgggctt cacaggcatt
     3421 gttcgccttt gtttcgttta ttaacaatgc gaagtggaag ttattaaaat gccaaaaatt
     3481 aagaccgtac gcggtgctgc taagcgcttc aaaaaaaccg gtaaaggtgg ttttaagcac
     3541 aagcacgcta acctgcgtca cattctgact aaaaaagcga ccaaacgtaa acgtcacctg
     3601 cgtccgaaag ccatggtttc caaaggcgat ctgggcctgg taatcgcgtg cctgccgtac
     3661 gcataagccg ttaacgtttt taacttttta attagaatat agatacagga gagcacatat
     3721 ggctcgcgta aaacgtggtg ttattgcacg cgcacgtcac aagaaaattt tgaaacaagc
     3781 taaaggctac tacggtgcgc gttctcgcgt ataccgcgtt gccttccagg ctgttatcaa
     3841 agctggtcag tatgcttacc gtgaccgtcg tcaacgtaag cgtcagttcc gtcaactgtg
     3901 gattgcgcgt atcaacgcag cagcacgtca gaacggtatt tcttacagca aattcatcaa
     3961 tggcctgaaa aaagcctctg ttgaaatcga ccgtaagatc ctggctgata tcgcagtatt
     4021 cgacaaagta gcgttcacca ctctggttga aaaagcgaaa gcagctctgg cataagccag
     4081 ttgaaagagg gagctagtct ccctcttttc gtttcaacgc catcaaaaca ttgactttta
     4141 tcgccgtagc cttttcaata aaggtctttt gaagagtacc aaaaggtaac gcaagcaatg
     4201 aatgctgcta ttttccgctt ctttttttac tttagcacct gaatccagga ggctagcgcg
     4261 tgagaagaga aacggaaaac agcgcctgaa agcctcccag tggaggcttt ttttgtatgc
     4321 gcgttttgaa aaataaaaag ccagtctatc aggcgtaatt gtcgcagcca gtctggacac
     4381 ggccagtgcg gagaaaccgg agcgtactta agtacgtgag gatttcgagc actgcccggg
     4441 accaaaatgg caagtaaaat agcctgatgg gataggctct aagtccaacg aaccagtgtc
     4501 accactgaca caatgaggaa aaccatgtca catctcgcag aactggttgc cagtgcgaag
     4561 gcggccatta gccaggcgtc agatgttgcc gcgttagata atgtgcgcgt cgaatatttg
     4621 ggtaaaaaag ggcacttaac ccttcagatg acgaccctgc gtgagctgcc gccagaagag
     4681 cgtccggcag ctggtgcggt tatcaacgaa gcgaaagagc aggttcagca ggcgctgaat
     4741 gcgcgtaaag cggaactgga aagcgctgca ctgaatgcgc gtctggcggc ggaaacgatt
     4801 gatgtctctc tgccaggtcg tcgcattgaa aacggcggtc tgcatccggt tacccgtacc
     4861 atcgaccgta tcgaaagttt cttcggtgag cttggcttta ccgtggcaac cgggccggaa
     4921 atcgaagacg attatcataa cttcgatgct ctgaacattc ctggtcacca cccggcgcgc
     4981 gctgaccacg acactttctg gtttgacgct acccgcctgc tgcgtaccca gacctctggc
     5041 gtacagatcc gcaccatgaa agcccagcag ccaccgattc gtatcatcgc gcctggccgt
     5101 gtttatcgta acgactacga ccagactcac acgccgatgt tccatcagat ggaaggtctg
     5161 attgttgata ccaacatcag ctttaccaac ctgaaaggca cgctgcacga cttcctgcgt
     5221 aacttctttg aggaagattt gcagattcgc ttccgtcctt cctacttccc gtttaccgag
     5281 ccttctgcag aagttgacgt catgggtaaa aacggtaaat ggctggaagt gctgggctgc
     5341 gggatggtgc atccgaacgt gctgcgtaac gttggcatcg acccggaagt ttactctggt
     5401 ttcgccttcg ggatggggat ggagcgtctg actatgttgc gttacggcgt caccgacctg
     5461 cgttcattct tcgaaaacga tctgcgtttc ctcaaacagt ttaaataagg caggaataga
     5521 ttatgaaatt cagtgaactg tggttacgcg aatgggtgaa cccggcgatt gatagcgata
     5581 cgctggcgaa tcaaatcact atggcgggcc tggaagttga cggtgtagaa ccggttgccg
     5641 gtagcttcca cggcgtggtc gttggtgaag tggttgagtg tgcgcagcat ccgaacgctg
     5701 acaaactgcg tgtgacaaaa gtgaatatcg gcggcgatcg cctgctggac atcgtctgcg
     5761 gtgcgccaaa ctgccgtcag ggcctgcgtg tggcggtagc taccattggt gctgttctgc
     5821 cgggtgattt caaaattaaa gcggcgaaac tgcgtggcga accgtctgaa gggatgctgt
     5881 gctccttttc tgagctggga atttctgacg atcacagtgg cattatcgaa ctgcctgcgg
     5941 atgcgccgat tggcaccgac atccgcgaat acctgaaact cgatgataac accatcgaaa
     6001 tcagcgtgac accaaaccgt gccgactgct taggtatcat tggtgttgcg cgtgacgttg
     6061 ccgtgctgaa tcagctgccg ctggttgaac cggaaatcgt tccggttggt gcgaccatcg
     6121 acgacacgtt gccgattaca gtcgaagcgc cggaagcctg cccgcgctat cttggccgtg
     6181 tggtaaaagg cattaacgtt aaagcgccaa ctccgctgtg gatgaaagaa aaactgcgtc
     6241 gttgcgggat ccgttctatc gatgcagttg ttgacgtcac caactatgta ctgctcgaac
     6301 tgggccagcc gatgcacgct ttcgataaag atcgcattga aggcggcatt gtggtgcgga
     6361 tggcaaaaga gggcgaaacg ctggtgctgc ttgatggcac tgaagcgaag ctgaatgctg
     6421 acactctggt catcgccgac cacaacaagg cgctggcgat gggcggcatc tttggtggcg
     6481 aacactctgg cgtgaatgac gaaacgcaaa acgtcttgct ggaatgcgct ttcttcagcc
     6541 cgctgtctat caccggtcgt gctcgtcgtc atggcctgca tactgatgcg tctcaccgtt
     6601 atgagcgtgg cgttgatccg gtactgcagc acaaagcgat ggaacgtgcg acccgtctgc
     6661 tgatcgacat ctgcggtggt gaggctggcc cggtaattga tatcaccaac gaagcaacgc
     6721 tgccgaagcg tgcaaccatc actctacgtc gtagcaaact ggatcgcctg ataggccatc
     6781 atattgctga tgagcaggta actgacattc tgcgtcgtct cggctgcgaa gtgaccgaag
     6841 gcaaagacga atggcaggca gttgcgccga gctggcgttt cgatatggag attgaagaag
     6901 atctggtcga agaagtcgcg cgtgtttacg gctacaacaa catcccggat gagccggtac
     6961 aggcaagcct gattatgggg actcaccgtg aagctgacct gtcgctcaag cgcgtgaaaa
     7021 cgctgctcaa cgacaaaggc tatcaggaag tgatcaccta cagcttcgtt gatccgaaag
     7081 tgcagcagat gatccatcca ggcgttgaag ccttactgct ggcaagcccg atctctgttg
     7141 aaatgtcagc aatgcgtctt tctctgtgga ccggcctgct ggcaaccgtg gtgtacaacc
     7201 agcaccgtca gcagaaccgt gtgcgcattt tcgaaagcgg tctgcgtttc gtaccagata
     7261 ctcaggcacc gttgggcatt cgtcaggatc tgatgttagc cggtgtgatt tgcggtaacc
     7321 gttacgaaga gcactggaac ctggcaaaag agaccgttga tttctatgat ttgaaaggcg
     7381 accttgaatc cgttctcgac ctgaccggta aactgaatga ggttgagttc cgtgcagaag
     7441 cgaatccggc actgcatccg gggcaatccg cagcgattta tctgaaaggt gaacgtattg
     7501 gttttgttgg agttgttcat cctgaactgg aacgtaaact ggatcttaac ggtcgcactc
     7561 tggtgttcga actggagtgg aacaagctcg cagaccgcgt ggtgcctcag gcgcgcgaga
     7621 tttctcgctt cccggcgaac cgtcgtgaca tcgcggtggt ggtcgcagaa aacgttcccg
     7681 cagcggatat tttatccgaa tgtaagaaag ttggcgtaaa tcaggtagtt ggcgtaaact
     7741 tatttgacgt gtaccgcggt aagggtgttg cggaggggta taagagcctc gccataagcc
     7801 tgatcctgca agataccagc cgtacactcg aagaagagga gattgccgct accgtcgcca
     7861 aatgtgtaga ggcattaaaa gagcgattcc aggcatcatt gagggattga acctatggcg
     7921 cttacaaaag ctgaaatgtc agaatatctg tttgataagc ttgggcttag caagcgggat
     7981 gccaaagaac tggttgaact gtttttcgaa gagatccgtc gcgctctgga aaacggcgaa
     8041 caggtgaaac tctctggttt tggtaacttc gatctgcgtg ataagaatca acgcccggga
     8101 cgtaacccga aaacgggcga ggatattccc attacagcac ggcgcgtggt gaccttcaga
     8161 cccgggcaga agttaaaaag ccgggtcgaa aacgcttcgc ccaaagacga gtaatctgat
     8221 ctaactaaaa aggccgctat gcggcctttt ttcttttcac tgtcgaagag tcaccgtaaa
     8281 atcaacgcca tgacacttca gcagaacgga taccatgctg acacttgccc gccaacaaca
     8341 gcgacaaaat attcgctggt tattatgcct gtcagttttg atgctgctgg cgcttctctt
     8401 aagcctttgc gccggtgaac aatggatttc gccaggtgac tggtttactc ctcgtggcga
     8461 actgttcgtc tggcaaattc gcctgccacg tacgctggct gtattgctgg ttggtgcggc
     8521 gctggctata tccggcgctg taatgcaggc gttgtttgaa aatcctctgg cagaacctgg
     8581 actacttggc gtctctaacg gcgcaggcgt ggggcttatc gccgcggtat tgcttgggca
     8641 agggcaactc cccaactggg cgctagggct gtgtgcgatt gctggcgcgc ttatcatcac
     8701 tttaatactc ttacgtttcg cccgtcgtca tctttcgacc agtcggttat tgctggctgg
     8761 cgttgcatta gggattatct gcagcgcact aatgacgtgg gctatctact tttccacctc
     8821 tgttgattta cgtcagctga tgtactggat gatgggcggt tttggcggcg tagactggcg
     8881 gcaaagctgg ctgatgctgg cattgatccc cgtgttgttg tggatctgtt gtcagtccag
     8941 gccgatgaat atgttagcac ttggcgagat ctcggcgcgg caactgggtt tacccatgtg
     9001 gttctggcgc aatgtgctgg tggcagcgac cggctggatg gttggcgtca gtgtggcgct
     9061 ggcgggtgct atcggcttta ttggtctggt gatcccacat attcttcggt tgtgtggttt
     9121 aaccgatcat cgcgtattac ttcccggctg cgcgctggca ggggcgagcg cattgctgct
     9181 ggccgatatt gtagcgcgcc tggcattagc tgccgcagag ctgcctattg gcgtggtcac
     9241 cgcaacgttg ggtgcgccgg tgtttatctg gttattgtta aaagcaggac gttagccgca
     9301 aaaagacggt ctatgattaa aagctaagat ttttactgac cacacctagg agaaacgatg
     9361 caagattcca ttctgacgac cgtagtgaaa gatatcgacg gtgaagtgac cacgctggag
     9421 aagtacgccg gtaatgtgct gttaattgtc aatgtcgcct caaagtgtgg cttaacgccg
     9481 caatatgagc agttggagaa tattcaaaaa gcctgggccg atcgaggttt tgtggtgctg
     9541 ggattcccgt gcaaccagtt tctggaacaa gaaccgggca gcgatgaaga gattaaaact
     9601 tactgtacca ccacatgggg ggtgacgttc ccgatgttca gtaagattga agttaatggc
     9661 gaaggacgcc atccgctgta tcaaaaattg attgccgcag cgccgaccgc agtcgcgccg
     9721 gaagagagcg gattctatgc ccgtatggtc agcaaaggcc gtgcaccgct gtacccggat
     9781 gatattttat ggaattttga aaaattcctg gttggcaggg acggaaaagt catccagcgt
     9841 ttttccccgg atatgacgcc ggaagatccc attgtgatgg aaagcattaa actggcgttg
     9901 gcaaaataat gtctattgtg atgcagttac aggatgttgc ggaatctacc cgcctggggc
     9961 cgctttcagg cgaggttcgg gctggggaaa tcctgcactt ggtggggccg aatggcgcgg
    10021 gtaagagtac cttactggcg caaatggccg gaataaccag cggtaaggga agcattcagt
    10081 tcgcggggca accactggaa gcatggtccg caacaaaact cgcgctgcat cgcgcctatc
    10141 tttcacaaca gcagacgccg ccgtttgcaa tgccggtctg gcactacctg acactgcatc
    10201 agcacgataa aacgcgtacc gaactactga atgatgtcgc aggggcgctg gctcttgatg
    10261 acaaactcgg tcgtagcacc aatcaacttt ccggcggtga atggcaacgc gtacgtcttg
    10321 ctgcggtggt gttgcaaatc acaccacaag ccaatcccgc aggccaattg ctgcttcttg
    10381 atgagccgat gaacagtctt gatgttgcgc aacaaagtgc gttagacaaa attctgagcg
    10441 cgctgtgtca gcaaggactg gcgattgtga tgagcagtca cgatctcaac cacacgttgc
    10501 gtcatgcgca tcgggcgtgg ttgctaaaag gtggaaaaat gctggccagt ggacgcagag
    10561 aagaggtgct cacgccgcca aatctggcgc aggcctatgg gatgaatttt cgccgtctgg
    10621 atatcgaagg tcacagaatg ctgatttcga ccatctgatt tcattagtat cccgaaaatc
    10681 gggtaattac gacgctaaat taatgcaaga ataaaaaaca gaggaaccgt acggaatgcg
    10741 tttctgcctt attttgatca cagcactgtt tctggccggg tgtagccacc ataaagcacc
    10801 gccgccaaat gccagacttt ctgattcgat taccgttatt gccggtttga acgaccagct
    10861 acaaagctgg catggcacgc cgtatcgtta tggtggcatg acgcggcgcg atcgtttcga
    10921 tttgcatctg ccccgtgaaa ccaaacaaca agcctctatt ggcacgcaaa ttgataaaga
    10981 cgagttgctg cctggtgacc tggtcttttt caaaacgggt tccggacaaa atggtttgca
    11041 tgtaggtatt tacgatacca acaaccaatt tatccacgcc tctaccagca agggagtgat
    11101 gcgttcctca cttgataatg tctattggca gaaaaatttc tggcaggcga gacgaatcta
    11161 gtatgtcgaa aaattgacgg cgatgataca tagccgtcaa tttactcctg aatacagtag
    11221 tattttgcag acaaaaattc cgtaaatgga ttattcgttt ataatcagga taatctaaaa
    11281 aaaggccccc tgaaaaagtg gttaacatgg aacaaatgaa agttactcct tctgtaattg
    11341 ttatgtctta tggcctcatc ccttttagcc ggatactgaa aaacatcctt cgagagggac
    11401 ggttaccatg aagatttttt tggagaatct ttatcattct gattgctatt tcctgccgat
    11461 aagagataac cagcaactcc ttgtcggcgt tgagttaatt acccatttct ccagtgaaga
    11521 tggcacggtt cgtatcccga cgagtcgggt tatagcgcaa cttaccgagg agcaacattg
    11581 gcaattattt tcagagcagt tggaattact aaaatcatgc caacactttt ttattcaaca
    11641 taaactcttt gcctggctta atttaacgcc acaagtggct acattattat tagacaggga
    11701 taattacgca ggcgaactat taaaatatcc gtttattgag ctgttgatta atgagaatta
    11761 cccgcatctc aatgaaggaa aagataatcg cgatcttttg tccttatcgc aaatgtatcc
    11821 ccttgtgtta ggcaatttgg gagcaggtaa tagcacaatg aaagcggttt ttgatggtct
    11881 tttcacccgc gtaatgctgg agaaaggttt tattcagcaa caaatcacgc atcgctcctt
    11941 cgaaccgttt attcgtgcga tccaggcgca aatttcccct tgttgcaatt gcatcatcgc
    12001 ggggggcatt gatacgccag aaatattggc acagattacc cccttcgatt tccatgctct
    12061 ccagggatgc ctgtggcctg ccgttcctat aaatcagata acgacgttgg ttcagcgata
    12121 accctcctgt ttgctggtgt ttaagacgag agtaaccgtc tacactatca aacagatagg
    12181 atctatgacc ctgtctttta ttacccgctg gcgcgatgaa ttgccagaaa cctatacagc
    12241 actttcccct acgcctttaa ataatgcccg gctgatttgg cataataccg aactcgctaa
    12301 cacgctgagt attccatcgt cgctgtttaa aaatgccgca ggtgtctggg gcggcgaaac
    12361 cctactgcct ggtatgtcac cactggcaca ggtttacagt ggtcatcagt tcgtcgtctg
    12421 ggcgggccaa ctgggtgatg ggcgcggcat tttacttggc gaacaactgc ttgctgatgg
    12481 cactacaatg gactggcatc tgaaaggtgc tggcctgacg ccttattcgc gaatgggtga
    12541 tggacgggcg gttttacgtt cgacgatacg agaaagtctc gccagtgagg cgatgcatta
    12601 tctgggcatt ccgacgaccc gcgcgttaag tatcgtcacc agcgattcgc cagtgtatcg
    12661 ggaaacggtg gagccaggcg cgatgctgat gcgtgtggca ccaagtcatc tgcgctttgg
    12721 tcatttcgaa catttttact atcgccgcga gccggaaaag gttcgtcagt tggctgactt
    12781 tgccattcgt cattactggt cacatcttga agacgatgag gacaaatacc gtctctggtt
    12841 taacgatgtt gtcgcacgta ccgcgtcgtt aattgcccaa tggcagacgg tcggctttgc
    12901 acatggggtg atgaataccg acaacatgtc gctgctggga ctgacgcttg attacgggcc
    12961 gtttggtttt cttgatgatt acgaacccgg ttttatttgt aatcactcgg atcatcaagg
    13021 gcgttatagc tttgataatc aacctgctgt cgcgttgtgg aatttacagc gtctggcgca
    13081 gacattgtca ccatttgttg ccgtagatgc cctgaatgag gccctggaca gctatcagca
    13141 ggttttgttg acgcattatg gacaacggat gcggcagaaa ctgggcttca tgacggagca
    13201 aaaagaggat aacgcgctac tgaatgaatt attcagtctg atggcgcgag agcgcagcga
    13261 ttatacccgc acattccgca tgctgagtct gaccgagcag cacagcgcgg cgtcaccgct
    13321 acgtgaggag tttattgatc gtgcggcatt tgatgactgg tttgcccgtt atcgggggcg
    13381 tttgcaacaa gacgaggtta gcgatagtga gcgtcagcaa ctgatgcaaa gcgttaaccc
    13441 agctctggta ttgcgcaact ggttggcgca acgggcgatt gaagcggcag aaaagggtga
    13501 tatgatggaa ttgcaccgcc tgcatgaggc gttgcgaaat cctttcagcg acagagatga
    13561 tgactatgtc agtcgtccac ctgactgggg taaacggctg gaagtcagct gctcgagtta
    13621 aaggctactt agttaacagc agcttgccag cctgagtttt acggagcaga tattcttgcc
    13681 cgtcatgatc gataatcagt ttgccatccg gacctaacag tgtctggctg gtaattcgcc
    13741 ggatgggctg cggggaagcg gtcttgtgat tggcatccgt ttcaggcgtg agttttctgc
    13801 tatccgtata acgcataaaa attaataata attgatataa caatgatttc tattatcaat
    13861 attatggcag gccagagcaa gcagtaatgt gttgagaaga caacaagttg cgcagaaatg
    13921 cccatcccat caggaatggg cacgcattca gaagcgggta tctaccgcaa aggcgagttt
    13981 ttcgaccagg cgttcggtat cttcccagcc cagacacggg tcggtaatgg attgaccgta
    14041 agtgagcggc tgaccgccga cgattttttg cgttccttcg cgcaggaaac tttccgccat
    14101 aattccagca atctccgtag agccattgcg gatttgctga caaatatcct cacaaacttc
    14161 taactggcga cggtgctgct tctggcagtt accgtggctg aaatccacca ccagatgttc
    14221 aggtaaatca aactcgtgca gcgtatcgca ggctgcggcg atatcatcgg catgataatt
    14281 cggttttttg ccgccacgca taataatgtg gccatacggg ttgccgctgg tctgatagat
    14341 ggtcatctga ccatttttgt ctggcgagag gaacatatgg ctggcgcggg ctgcgcggat
    14401 agcatccacg gcaatccgcg tgttgccatc ggtaccattt ttaaaaccta ccggacagga
    14461 gagtgctgaa gccatttcgc ggtggatctg actttcggta gtacgagcgc caatcgcgcc
    14521 ccaactgatt aaatcagcaa taaactgacc ggttaccata tcgaggaact cagtcgcggt
    14581 agggacaccc agctcattta cctgtaaaag taatttgcgc gccagctcca gaccgtgatt
    14641 tacccgatag ctgccgttta aatccggatc ggagattagc cctttccagc cgacaacagt
    14701 tcgtggtttt tcaaaatagg tgcgcattac gatttccagc cgtgactggt actggttgcg
    14761 cagcgactgc agacgggtgg cgtactctat tgcagcggtg agatcgtgga tcgagcaggg
    14821 gccaataatg accaacagtc gcttatcttc accattcagt attttttcaa ttctgcggcg
    14881 ggagtcggtg acatgggtgg cgacgccagg cgttacggga taccgtagcg cgagttcggc
    14941 gggcgttacc aggctctcaa tacgcgcagt acggagttcg tcagttctgt tcattacaag
    15001 tctcagaaat ttgttgcttc tacggcagga ctaccggaaa gtgatgcgca tcacgataaa
    15061 ccaatcgcag ctgatttcaa gtgcgaggtg tgtcacacct cgccgggtgg ttagcatgat
    15121 aacaaaaaaa caaaccaatg cactagttct ctagtacatt cggcgactaa ggcccatgat
    15181 atcgaggatc ttggtggcaa tctcttctac cgaataattg gtactgttaa tccacgggat
    15241 ctgattttta cggtacaagg cttccacttc cgcgacttcc atcctgcact gacgaagcga
    15301 ggcatagcga ctgttctccc gacgttcctc gcgaatcgcc gccagacgtt ccgggtcgat
    15361 agtcaggccg aacaatttat gctgaagcgg tttgagcgac gcgggtagca ccagattatc
    15421 catatcgtcg gcaataaagg ggtagtttgc cgcgcggata ccaaattgca ttgccagata
    15481 cagactggtg ggggttttac cacagcgaga aacaccgagc aggatcacct gagcctgatc
    15541 cagattgcgc aacgaaatgc cgtcatcgtg ggcgagggtg taatcaatcg ccgcaatgcg
    15601 cgcatcatat ttattgagat tattagggtt aaggccatgg gtacgatgag caatcggcgt
    15661 tggatccagt ttcatctctt gttgtagcgg ggcaaccagc gcctgaacga tatcctggca
    15721 aaagccttca ctttgcaaga tgatggcgcg aatctccggc aacacgatgg agtagaagac
    15781 cagcgggcgc acgcctgtct ggtgataaat tgcgtcaatc tgatccttca ctgcccgtgc
    15841 acggctctca ttttcgacaa acggcagcgt gatgctgctg atagtgacgg gaaattgtga
    15901 cattactgcg tgtcctaata cctccgcagt tattgccgta ccatcagaaa tataaaaaac
    15961 gtggcgatca acagcattat ccattttgtt cttcccgtga agcagacata attctatgaa
    16021 agcataaatt aaaagcgcac agaagcgtag gacgtaatgt ctggtttata aaatgaacct
    16081 tcattttatt ttttataaaa acagcatttc atttttatgg tttcgtttat accgatggtt
    16141 tatgtggaaa ttgtcgaata gagcaggttt gcgcaacgct gggatcagtc ttaaaaagta
    16201 aaaaaatata tttgcttgaa cgattcaccg tttttttcat ccggttaaat atgcaaagat
    16261 aaatgcgcag aaatgtgttt ctcaaaccgt tcatttatca caaaaggatt gttcgatgtc
    16321 caacaatggc tcgtcaccgc tggtgctttg gtataaccaa ctcggcatga atgatgtaga
    16381 cagggttggg ggcaaaaatg cctccctggg tgaaatgatt actaatcttt ccggaatggg
    16441 tgtttccgtt ccgaatggtt tcgccacaac cgccgacgcg tttaaccagt ttctggacca
    16501 aagcggcgta aaccagcgca tttatgaact gctggataaa acggatattg acgatgttac
    16561 tcagcttgcg aaagcgggcg cgcaaatccg ccagtggatt atcgacactc ccttccagcc
    16621 tgagctggaa aacgccatac gcgaagccta tgcacagctt tccgccgatg acgaaaacgc
    16681 ctcttttgcg gtacgctcct ccgccaccgc agaagacatg ccggacgctt cctttgccgg
    16741 tcagcaggaa accttcctca acgttcaggg ttttgacgcc gttctcgtgg cagtgaaaca
    16801 tgtatttgct tctctgttta acgatcgcgc catctcttat cgtgtgcacc agggttacga
    16861 tcaccgtggc gtggcgctct ccgctggtgt tcagcggatg gtgcgctccg acctcgcatc
    16921 ttctggcgtg atgttctcca ttgataccga atccggcttt gaccaggtgg tgtttatcac
    16981 ttccgcatgg ggccttgggg aaatggtcgt gcagggtgcg gttaacccgg atgagtttta
    17041 cgtgcataaa ccgacactgg cggcgaatcg cccggctatc gtgcgccgca ccatggggtc
    17101 gaaaaaaatc cgcatggttt acgcgccgac ccaggagcac ggcaagcagg ttaaaatcga
    17161 agacgtaccg caggaacagc gtgacatctt ctcgctgacc aacgaagaag tgcaggaact
    17221 ggcaaaacag gccgtacaaa ttgagaaaca ctacggtcgc ccaatggata ttgagtgggc
    17281 gaaagatggc cacaccggca aactgttcat tgtgcaggcg cgtccggaaa ccgtgcgctc
    17341 acgcggtcag gtcatggagc gttatacgct gcattcacag ggtaagatta tcgccgaagg
    17401 ccgtgctatc ggtcatcgca tcggcgcggg tccggtgaaa gtcatccatg acatcagcga
    17461 aatgaaccgc atcgaacctg gcgacgtgct ggttactgac atgaccgacc cggactggga
    17521 accgatcatg aagaaagcat ctgccatcgt caccaaccgt ggcggtcgta cctgtcacgc
    17581 ggcgatcatc gctcgtgaac tgggcattcc ggcggtagtg ggctgtggag atgcaacaga
    17641 acggatgaaa gacggtgaga acgtcactgt ttcttgtgcc gaaggtgata ccggttacgt
    17701 ctatgcggag ttgctggaat ttagcgtgaa aagctccagc gtagaaacga tgccggatct
    17761 gccgttgaaa gtgatgatga acgtcggtaa cccggaccgt gctttcgact tcgcctgcct
    17821 gccgagcgaa ggcgtgggcc ttgcgcgtct ggaatttatc atcaaccgta tgattggcgt
    17881 ccacccacgc gcactgcttg agtttgacga tcaggaaccg cagttgcaaa acgaaatccg
    17941 cgagatgatg aaaggttttg attctccgcg tgaattttac gttggtcgtc tgactgaagg
    18001 gatcgcgacg ctgggtgccg cgttttatcc gaagcgcgtc attgtccgtc tctctgattt
    18061 taaatcgaac gaatatgcca acctggtcgg tggtgagcgt tacgagccag atgaagagaa
    18121 cccgatgctc ggcttccgtg gcgcgggccg ctatgtttcc gacagcttcc gcgactgctt
    18181 cgcactggag tgtgaagcag tgaaacgtgt gcgcaacgac atggggctga ccaacgttga
    18241 gatcatgatc ccgttcgtgc gtaccgtaga tcaggcgaaa gcggtggttg aagaactggc
    18301 gcgtcagggg ctgaaacgtg gcgagaacgg gctgaaaatc atcatgatgt gtgaaatccc
    18361 gtccaacgcc ttgctggccg agcagttcct cgaatatttc gacggcttct caattggctc
    18421 aaacgatatg acgcagctgg cgctcggtct ggaccgtgac tccggcgtgg tgtctgaatt
    18481 gttcgatgag cgcaacgatg cggtgaaagc actgctgtcg atggctatcc gtgccgcgaa
    18541 gaaacagggc aaatatgtcg ggatttgcgg tcagggtccg tccgaccacg aagactttgc
    18601 cgcatggttg atggaagagg ggatcgatag cctgtctctg aacccggaca ccgtggtgca
    18661 aacctggtta agcctggctg aactgaagaa ataaaataaa tccccggcgg cgttcagtcg
    18721 ccggggttat gtgatccccg aagatgaaac ttattcaatc tcttcacaga catcctgcgt
    18781 taatcgccgt ataatatctt ttcttaacaa aaacttttgt attttatctg aggcagtgcg
    18841 cggtagtttt tcgattacca cgatatgttc aggatattta tattttgcga cccgtttacg
    18901 gctaaaaaaa gccgccatct cttccagcga taatgaatga tgcggcgctt tcagcacgac
    18961 ataagcgcat gatcgttcac ctaaacgttc atcgggcatt gcaaccacac aggcatcgtg
    19021 aattttagga tgctgcaata aaatatcttc cacttcacgg ctgctaatat tttcgccgcc
    19081 gcggacaata atatcttttt tgcgtccggt aatttttata tagccagcct catccatacg
    19141 gcagagatcg ccgctgtaat accagccttc ttcatccagc gcacgggcgg ttaattcagg
    19201 ttcatcaaaa taccccataa acacattggg gccacgcgat gcttcttcac cttcgcaacc
    19261 tggcggtaag gtcttgcgtg cgtcatcgac cactttaatc tctacacctg cggcagcgta
    19321 accatcggta tgcataaagc gcgacaaaga attatcgaga ttcaccaccg catgcggcga
    19381 actttctgtg gaaccataga cacttaataa tttaatgctg cgctgctggc attcgcgcgc
    19441 cacttttttg gggattgtgg taccgccgca aagaaagaag cgcagcgctg aaaggtctgc
    19501 gggttgtttc tctagtaaat tcaaaagatc atagacaaac ggcgttgcgc ctagcataca
    19561 ggtgcaacgc tgctgctcaa gcagcgcgag acacgcatca ggagtgaaaa tatctaacaa
    19621 cacgctgcga gcgccaatta agaatggtgc cgttacgcca tgcagaaagc ccgttgcgtg
    19681 accaagtggc gcaggcatca taaagacatc cagccaggtc agattcagcc gcgcgcaata
    19741 agcccgctca ctggcgagaa tattgttatg cgttagcatc acgccctttg gcagaccctc
    19801 ggttccggag gtaaacagca ccgcagctaa ttcatcgccg tgggtcgtta tcgccgtggt
    19861 cagtgggata ttgtcggcga taatctgact taatgagagg gaagaggtgg cgggagccag
    19921 tttgtccacg ccgacaattt gttgtagttg tggaagctga ttttgcagcg gcaggattaa
    19981 atctaccgga cgcgtttgtt taaacaacgt cggtgcaaag aacatttttg cctgacactt
    20041 attgagcacc cacaccagtt ctgcttcccg ccaggaaggc aacagcggca cggaaaccgc
    20101 accgattttc aggcaggcaa gatagataac ggtaaattca caccagccag gcagttgaaa
    20161 tgcgatgcga tcgcctgatt caataccctt cgctaacatc cagtttgcca gacagctcgc
    20221 ggcgtgatcg agcgcgctat aggtgtacga tgcaccatga ttatcgacca cggcaatttt
    20281 gtctggcatc gcacgagcgg tctgctgcca gtaatcggcc agtgaagcat cgccccatag
    20341 cccttgctga cgatacgccg cacgacgttg ttcgttaaac gttaatgtca ctttcatttt
    20401 ggactctcga aacagaacat caggcccgag ttgcgggcct gtgggatgca tgattagccg
    20461 tagcgaaaat caataccgaa ggtgcctgcg ggatattgcc actgttcgag aatagtgtta
    20521 ccgcacagca cccgacaggt gccgcactcc agacaaccgg cgggatcaaa atgaatgttt
    20581 cctacgtcat cctgcttata aagtccggca gggcaggctt tcattaattt acggaattca
    20641 ttgatatcgg gattttccgt cagaatgata tgcgggtggc cctcatcaac atggaattta
    20701 ttgacgccta atttgatgtc gacgttaacc gtagcgttct ggctcatagc gcggttgctc
    20761 ccttaatgcc atctttcagc aagttgatca gcccaatttt cttcgcgtgt cccatgatca
    20821 ttttgcgtac cggctggttt ggtttgccgt caatggtgaa catctcgttc atgatgtcgg
    20881 ctaccattcg tgggtattgg ctaaacaggc gcgggttttc catcagcgcc gggatcttgc
    20941 gaaaatgctg catatcacgc atgacgcagc tttgttccag ctcacgtttg tattgcgcca
    21001 gactgttggc ggagaaatcc gcgcgttctt tggcggcgat caccgttgtg gcggcagcct
    21061 gagccgatgc aatggctaaa tccatgccgc ggaccgtaaa acccaaattc aggcagaagc
    21121 ctgcggcgtc accaacgatc atcacgccct cgttaaccag ttgcggcacc attgccagac
    21181 cgccttccgg caccatatgc gcggaatatt caagcagttt gccgccgcta atcagcgggc
    21241 gaatggcggg gtgttgttta aaatcttcca gcatttgcgg cacgcttttt tgcgcatggg
    21301 cgatatcacc caatccacaa accagcccca aggatatgga atccttgttg gtatagagaa
    21361 atcccccgcc catcaggccg tcagaagggg aaccggcaaa cagccaggcg gcaccttcct
    21421 cgcccgtaat attaaagcga tcgttgatct gttctggtgt gaggccaata acctctttaa
    21481 caccaacagc gtaatgatgc ggatcggaag cgggaaccat tcccagcgag cggccaagca
    21541 tcgagttaac gccatcagcc agaatcacca cattcgcttc gagaatatca tccccagcct
    21601 gcacgccagt gaccttgttt ccttcacgaa ccagcgcgtc gacgcgaacg cctgggataa
    21661 actgcgcgcc cgcctgctcg gcttgttcca tcaaccacgg gtccagacga ttacgcaata
    21721 cggtataaga tgcgtgttga ggaacatctg gttgctcgcg gtgaaaatcg agggtaacgg
    21781 cgctttcttc ggttaagaag gagattttct cgcgtgtgac cttgcgttct accggcgctg
    21841 atactgcaaa gcctggaatg attgcttcaa gtgtgtgggc ataaagacgc ccgccggtca
    21901 tgtttttaca tccggcactg tcgccgcgtt ctatcaccag gacatccagc ccggctcgtg
    21961 ccatgaccag tgcggcaacg ctaccagcaa cgcccgcacc gaccacaatg gcatcaaatt
    22021 tgtcatccga catgcgttat gctcccttcc tgctgcgttt acagcgggag atgtggttga
    22081 aaggggaatt agcggctcaa ctggctaatc agggcaggga cgactttgta gatatcgccc
    22141 accagaccgt agtcggcata gttgaagatt ggcgcatttt tatctttatt gatggcgaca
    22201 atcactttta cgccgttgcc gccaaccata tgttggatct gcccggagat ccccagcgtc
    22261 aggtagagat cggatttcag caacacgccg gagacaccga tataacgttc acgctccatc
    22321 cagttctcgc cttcggcaat tggacgtgaa cagccgactt cagcattcag caccgccgcc
    22381 agttcgtgga ccattttcag gtcatcctgc gccgccagac cacgaccgac gccaaccaca
    22441 cgttttgctt tgctcaggtc cacgctgctt gcggctttcg cacggcgttc gcgacagaga
    22501 atttcatgac gcggagcaac ataaggtacc gtttctgtcg ggcactgatg agaggtatca
    22561 ctggtgcacg gttcctgaac accgggtgca agggtaatga tcgccagcgg gctgttgatc
    22621 ttttcctaag cgaacgctaa cccgccatac atccggtgtt cggcgcaaat gtgaccatcg
    22681 acaatatcca ccgccgtggc atcgttcacc agcgccgcat tcagttgcac acttaaccgt
    22741 gctgccagag ctttaccacg tttcgtcgcg gccaacagca acatagcggg gtgtttatct
    22801 ttcagcaggg cagcaatgct ttcggcgtaa ttttcagtgc gttgcagcgc gtcgttttgc
    22861 tcaagaacat aaatacattt tggaccataa ggcataactg cctgcgcctg gtcggtattt
    22921 tgtacaatgg catacacctg ttggccccat tgctgagcgc cgccaaacag ttcagcataa
    22981 cgttcaggat tatcgctaaa gacccagacg ctgtttaatt gactcatcgt ttcttctccg
    23041 taggcttcag ttcagggctt tcttcagatg ttccgccagc tcggcaatgg cctccggcga
    23101 atcgttatcg agaatgatgt gcttacgttc tgtttgcggc ggtacgcgaa tgccgaccag
    23161 ttcagcaagt ggcgcgctct ggctccagtc aatatcactt gcctgccact gatttaccgg
    23221 ttttttaccc gcgccgagaa tggctttcat cgaaggaatg cgtggcacgt taatatcgga
    23281 ggtgacgcag agcacggctg gaacagagag ttcaataact tcaacatcat cttcaagcgt
    23341 gcgttcaatc accagtgtat tgccctgacg ctgaatagca ctcactgcat taatcaccgg
    23401 aagttgcaga atttctccga ccagcaagcc aacctgctgg gcataaaggt cgccggaacc
    23461 ttcaccaaag atcagtaaat cgaagccgat cttttcaatt gctgccgcca gcgcttttgc
    23521 ggtatcgaga ggcagtgcat gttcaagttg cgcatcctgc accaaataca ggctgtgcgg
    23581 cccgcgggat agcacgtctt tgcgcacttt cgagttctgc aacaatgagc cgccaacggt
    23641 cagcgcggct atctcatcgt catctgttgc gagctggctt gcagcttcaa tggcattgag
    23701 atcgaactgg ctgattttgg cgtcggcatt gtcgaaattc agggtgtatt ctggagtgac
    23761 aacaatgtcc tgttcttcag gcaccagctt aaagcaggtt attattttca tgatttctcc
    23821 tgtgagtcgc atacggaacc atctccgatg gaaggtaaaa atggcctgaa aatagtgtgc
    23881 gatagcggtg cggggggttc gacggagcaa gctgttttcc ttgccaacaa tcttgcaaaa
    23941 aaaatgcgct tgctaaaaca aaagtgcaat ttgcggcaat atagttattc catgaccgta
    24001 caatcttgca ttcagtgtaa ataatcttgt ttagctctcg ctaaacgcgg ttgtataacc
    24061 gcaatacacg ccgtatttta tccggcatat attgtttttg cttgctcatg gaatagggag
    24121 gttgtcatgt atcaacgctg ttttgataat gccagtgaaa cgctgtttgt cgccggtaaa
    24181 acgccacggc tttcacgttt tgcatttagc gatgatccaa aatgggagtc tggacatcac
    24241 gttcatgaca atgaaaccga gctgatttac gtcaagaaag gggttgcaag gtttaccatc
    24301 gattcttcgt tatatgtcgc acatgcggat gacattgtgg tgatagaacg cggcaggctg
    24361 catgcggtgg cctctgacgt taacgatccg gcaacgacgt gtacctgtgc gctgtacggc
    24421 tttcagtttc agggggctga ggaaaatcag ttactgcaac cgcattcttg tccggtaatt
    24481 gccgcggggc agggaaaaga agtcataaaa accttattta atgagctaag tgtgattttg
    24541 ccgcaaagta aaaatagcca aacatcttcg ttatgggatg catttgccta tacattagca
    24601 attc
//
LOCUS       DAEEUE010000056        24482 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.056, whole genome shotgun sequence.
ACCESSION   DAEEUE010000056 DAEEUE010000000
VERSION     DAEEUE010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24482
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.056"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(84..881)
                     /locus_tag="KLK84_002422"
                     /pseudo
     CDS             complement(84..881)
                     /locus_tag="KLK84_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429194.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            864..1973
                     /locus_tag="KLK84_002423"
     CDS             864..1973
                     /locus_tag="KLK84_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBE2009713.1"
                     /translation="MVNVSHIRTLSSVESTTSSPSIRLTVESIWNYAGNTLVNQHSDI
                     NKTHEILQDESKCEMIVVIENFMTSSAKYADILLADLMTVEQEDIIPNDYAGNMGYLI
                     FLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPAL
                     PSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWE
                     LEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQE
                     VWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEPNMSGD
                     KIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            2034..4072
                     /locus_tag="KLK84_002424"
                     /pseudo
     CDS             2034..4072
                     /locus_tag="KLK84_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            4083..4700
                     /gene="dmsB"
                     /locus_tag="KLK84_002425"
     CDS             4083..4700
                     /gene="dmsB"
                     /locus_tag="KLK84_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBE2009714.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            4702..5556
                     /locus_tag="KLK84_002426"
     CDS             4702..5556
                     /locus_tag="KLK84_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HBE2009715.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            5599..6213
                     /gene="dmsD"
                     /locus_tag="KLK84_002427"
     CDS             5599..6213
                     /gene="dmsD"
                     /locus_tag="KLK84_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HBE2009716.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            6372..7664
                     /gene="clcB"
                     /locus_tag="KLK84_002428"
     CDS             6372..7664
                     /gene="clcB"
                     /locus_tag="KLK84_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HBE2009717.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALVG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(7617..8312)
                     /gene="bioD"
                     /locus_tag="KLK84_002429"
     CDS             complement(7617..8312)
                     /gene="bioD"
                     /locus_tag="KLK84_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBE2009718.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(8437..9657)
                     /gene="mlc"
                     /locus_tag="KLK84_002430"
                     /gene_synonym="dgsA"
     CDS             complement(8437..9657)
                     /gene="mlc"
                     /locus_tag="KLK84_002430"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HBE2009719.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(9792..10694)
                     /locus_tag="KLK84_002431"
     CDS             complement(9792..10694)
                     /locus_tag="KLK84_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE2009720.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAKEPFVFFDPHVGTGLYDDILGLMRR
                     YHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWL
                     VWPKHHEQSPAARNFRIHLLNALR"
     gene            10801..11610
                     /locus_tag="KLK84_002432"
     CDS             10801..11610
                     /locus_tag="KLK84_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE2009721.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFC
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSP"
     gene            11626..11883
                     /locus_tag="KLK84_002433"
     CDS             11626..11883
                     /locus_tag="KLK84_002433"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009722.1"
                     /translation="MVGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMPFGLL
                     MTLFSSLWLIFAGMLLFSAGFFAAHSVASSWIGPRAKRAKG"
     gene            12451..12786
                     /locus_tag="KLK84_002434"
     CDS             12451..12786
                     /locus_tag="KLK84_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HBE2009723.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            12879..12962
                     /locus_tag="KLK84_002435"
     CDS             12879..12962
                     /locus_tag="KLK84_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009724.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            13062..13883
                     /locus_tag="KLK84_002436"
     CDS             13062..13883
                     /locus_tag="KLK84_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HBE2009725.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(13922..14251)
                     /gene="mdtI"
                     /locus_tag="KLK84_002437"
     CDS             complement(13922..14251)
                     /gene="mdtI"
                     /locus_tag="KLK84_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HBE2009726.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(14238..14603)
                     /gene="mdtJ"
                     /locus_tag="KLK84_002438"
     CDS             complement(14238..14603)
                     /gene="mdtJ"
                     /locus_tag="KLK84_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HBE2009727.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            15014..16048
                     /gene="tqsA"
                     /locus_tag="KLK84_002439"
     CDS             15014..16048
                     /gene="tqsA"
                     /locus_tag="KLK84_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HBE2009728.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTWTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(16073..17461)
                     /gene="pntB"
                     /locus_tag="KLK84_002440"
     CDS             complement(16073..17461)
                     /gene="pntB"
                     /locus_tag="KLK84_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HBE2009729.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(17472..19004)
                     /gene="pntA"
                     /locus_tag="KLK84_002441"
     CDS             complement(17472..19004)
                     /gene="pntA"
                     /locus_tag="KLK84_002441"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HBE2009730.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            19528..20472
                     /locus_tag="KLK84_002442"
     CDS             19528..20472
                     /locus_tag="KLK84_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBE2009731.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20658..22040
                     /locus_tag="KLK84_002443"
     CDS             20658..22040
                     /locus_tag="KLK84_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBE2009732.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            22077..22799
                     /gene="folM"
                     /locus_tag="KLK84_002444"
     CDS             22077..22799
                     /gene="folM"
                     /locus_tag="KLK84_002444"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HBE2009733.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(22796..23131)
                     /locus_tag="KLK84_002445"
     CDS             complement(22796..23131)
                     /locus_tag="KLK84_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HBE2009734.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            23260..24003
                     /gene="rstA"
                     /locus_tag="KLK84_002446"
     CDS             23260..24003
                     /gene="rstA"
                     /locus_tag="KLK84_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HBE2009735.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            24228..>24482
                     /locus_tag="KLK84_002447"
     CDS             24228..>24482
                     /locus_tag="KLK84_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBE2009736.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLEN"
BASE COUNT         5990 a         6266 c         6019 g         6207 t
ORIGIN      
        1 cttattatgc ttccatcgga aacgatgatc tggcagcctg agttcacaga taaaacactc
       61 tccaggaaac ccggggcggt tcacagggac gctgacaagt ccttaaagct ggattgtttc
      121 agtgtctgga ccttctcagc cagcagtgat gtcaccaatt cctgaaaaag tacagatgat
      181 tctcgtgact ctggagtgag taactgacgt tcctgtgtga aaactgctgg tctatccccc
      241 tgctcattct tcagaatcga cgtagagtcg cctatagttg agtaatcagt atggttatta
      301 tcatcagtaa gctggcttac gccaccatca atcagcttaa tcagaccaga ggtatcacca
      361 gtgtcgactc tctgctgtgc tgcggtcagg acttccagcg ctaacttaat gcccctctgc
      421 tggtccacac tcaacgctct tgtcgctgca agttccctga ggttggactt ctcgtcaacc
      481 agtgtcgcca cctctacgcc attccagtag tcatcactgg catcagtcag gaactgctca
      541 gccatggacc gtatgtgcgc tgtaagctcc tgtagtgtga cttcaggatt gtccagtagg
      601 aatgccttag ctgtggctgc gagctctctc tgacgcattg tagcggtcct ccggtctgtg
      661 ctgcgggttg agactgatag aaacgggctg gtagctcctt taagtctgaa gcggacagtg
      721 taacgcccgt tggttctccg gactagctgt ggcgtagcaa ggtccttgat ttgtgtcagc
      781 cttgtgtcag gacttgaggt gtagacttgc tgatacagat agggagaccc agagaaagaa
      841 ctggatggat atgcagtatc gtcatggtta atgtcagtca tatcaggacc ttaagtagcg
      901 ttgagtcaac tacttcaagt ccgtccatcc gtctgacagt agagtccatc tggaactacg
      961 cgggaaatac cctcgttaat cagcattctg acatcaacaa aacgcatgaa attctgcagg
     1021 acgaatcgaa atgcgaaatg attgtggtca tcgaaaactt tatgacctca tcggcaaaat
     1081 atgccgacat tctgctggca gacctgatga ccgttgagca ggaagatatt attcctaacg
     1141 actacgccgg taacatggga tatctcattt tcctccagcc agtcaccagc gaaaaattcg
     1201 aacgtaaacc gatttactgg atcctgagtg aagtcgcgaa acgtcttgga cctgacgtct
     1261 atcaaaagtt cacagaaggt cgcacgcagg aacaatggtt acaacatctg tacgccaaaa
     1321 tgcttgccaa agatccggcg ttaccgtctt acgacgaact gaaaaaaatg ggtatctata
     1381 agcgtaaaga tcccaatggc cattttgtcg cctacaaagc atttcgtgac gaccccgagg
     1441 caaatccact taaaacgcct tccggtaaga ttgaaattta ttccagcaag ctggcggaaa
     1501 ttgcccgtac ctgggaactg gaaaaagatg aagtgataag cccactgcca gtttatgcct
     1561 caacctttga aggctgggac tcccctgaac gtagaacctt cccactgcaa ctgtttggtt
     1621 tccattacaa atcccgtact cactcgacct acggcaacat tgatgtcctg aaggcagcct
     1681 gccgtcagga ggtgtggatc aaccctatag atgcacagaa acgtgggatt gccaacggcg
     1741 atatggtgcg ggtgtttaac catcgtggcg aagttcggct accagccaaa gtaacaccac
     1801 gcattctccc tggagttagc gctatgggcc agggagcctg gcacgagccc aatatgtctg
     1861 gcgataaaat cgaccatggc ggatgtgtga atacgctaac cactctgcgc ccttcaccac
     1921 tggcgaaggg aaacccgcag cacactaatc tggtcgagat cgaaaaaata taacccacta
     1981 caaccataaa ttctggcatg acattttgtt tgaaaagcaa taagtgagta atgatgaaaa
     2041 tccataccac agaggcgctc atgaaggctg aaatcagcca tagaagtctg atgaaaacct
     2101 ccgcacttgg cagtctcgcg ctggcaagca gtgctttcac tctgccattt tcccaaatgg
     2161 tccgggcggc agaggctccg gtagaagaga aaacggtctg gagttcctgc accgttaact
     2221 gcgggagccg ctgtctgcta cgtttgcatg tgaaagatga caccgtgtac tgggtggagt
     2281 ctgatacgac aggtgacgac gtttacggta atcatcaggt tcgggcatgt ttacgcgggc
     2341 gctctatccg ccgacggatg aatcatcctg acaggttgaa atatcccatg aagcgcgtcg
     2401 gcgagcgcgg tgaaggtaaa tttgaacgga taagttggga cgaagccctg gataccatca
     2461 gtgataatct tcggcggatc ctgaaagatt acggcaatga ggctgtacat gtcctgtacg
     2521 gaacaggcgt agatggcgga aatatcacca actcgaacgt cccgtaccgt ctgatgaact
     2581 cttgcggggg ttttctcagt cgctatggca gctacagtac cgcacagatc agtgccgcaa
     2641 tgagttatat gttcggtgcc aatgatggca acagcccgga tgatatcgcc aatacgaaac
     2701 tggtcgttat gttcggaaat cacccggcag aaacgcggat gagcggcggt ggtgtcactt
     2761 actacgtcga gcaagcccgc gaacgttcaa acgcacgcat gatcgtcatc gatccacgct
     2821 ataacgacac agctgccggg cgtgaagatg aatggctgcc cattcgccct ggcaccgatg
     2881 gcgcactggc ctgtgcgatt gcctgggtac tgattactga aaacatggtc gatcagccat
     2941 ttctcgacaa atattgtgtt ggttacgatg aaaaaacgct gcccgccaac gcaccacgta
     3001 acgcgcatta taaagcctat attctgggcg aagggcctga cggcatagct aaaacgccgg
     3061 aatgggcagc aaaaatcacc agcatcccgg cagaaaaaat tatccagttg gcacgagaga
     3121 tcggttcagc aaaacctgct tatatttgtc agggttgggg gccacaacga cactccaacg
     3181 gcgaacaaac gtcccgcgct attgccatgc tttccgttct taccggtaac gtcggcataa
     3241 acggcggcaa ctcaggtgta cgcgaaggta gctgggatct gggggtagaa tggttcccga
     3301 tgctcgagaa tcctgttaaa acgcagattt ccgtttttac atggacagat gccatcgacc
     3361 atggtacgaa aatgaccgcg acccgtgatg gtgttcgtgg aaaagaaaaa ctggatgtcc
     3421 ccatcaagtt tttatggtgc tacgccagta acacattgat caatcaacat ggcgacatca
     3481 atcacaccca tgaggtgctt caggatgaca gcaagtgcga gatgattgtt ggcattgacc
     3541 acttcatgac ggcctcggct aagtattgcg atatcctgtt gcctgacctg atgccaacag
     3601 agcaagaaga ccttatctct catgaatctg cagggaatat gggctatgtg atcctcgccc
     3661 aacccgcaac ctcagcaaaa tttgaacgca aacccatcta ctggatgctg agtgaagtcg
     3721 ccaaaccaaa gcgcgtaccc actccagcta cggcaatatt gatgtgttac agcaggcctg
     3781 cccacaagag gtgtggatca accccattga tgctcaggca cgcggtatcc gtcatggcga
     3841 taccgtgcgg gtatttaaca ataatggaga aatgctgatt gccgcaaaag tgactccgcg
     3901 tattctgcct ggcgttaccg ccatcgggca aggtgcgtgg cttaaagcgg atatgtttgg
     3961 tgaccgggtc gatcacggcg gcagtatcaa tattctgacc tctcaccgcc cttcaccgct
     4021 ggcaaaggga aacccgtcgc acagcaatct tgtccagatc gaaaaggttt aaggagtagc
     4081 ccatgaccac acaatatgga ttttttatcg attccagccg ctgtactggc tgtaaaacct
     4141 gcgaactggc gtgcaaagat tttaaagatc ttggcccgga agtcagtttc cgccgtattt
     4201 atgaatatgc tggcggcgac tggcaggaag ataatggcat ctggcaccag aacgtgttcg
     4261 cttattatct ctccatctcc tgtaaccatt gcgatgaccc cgcatgtaca aaagtctgcc
     4321 ccagcggcgc aatgcataag cgtgaagatg gctttgtggt ggttgatgaa gatgtttgta
     4381 tcggctgccg ctactgccac atggcttgtc cgtacggcgc accacagtac aatgctgaaa
     4441 aagggcacat gacgaagtgc gatggttgtt attcgcgcgt cgccgagggg aaacaaccca
     4501 tatgcgtcga atcctgcccg ctgcgtgcac ttgagttcgg tccaattgaa gagctgcgcc
     4561 agaagcacgg cactctggct gccgtcgcgc cactgccgcg cgcgcatttc acaaaaccca
     4621 atatcgttat caaacctaac gccaacagcc gcccgaccgg cgataccact ggttatctgg
     4681 ctaatccgga ggaagtgtaa aatgggaaat ggatggcatg aatggccact ggttatcttt
     4741 accgtattgg gtcaatgcgt agtaggcgcg ctgattgtta gcggaattgg ctggttcgcc
     4801 gccaaaaatg acgcagaccg tcagcacatt gttcgtggca tgtttttcct ctggctgttg
     4861 atgggcatcg ggtttattgc ctctgtcatg cacctgggat cgccactgcg agcgtttaac
     4921 tcgctgaatc gaatcggagc ttctggtctg agtaacgaga ttgctgccgg ttccatattt
     4981 tttgccgttg gcggactatg gtggctggtg gcggtcatcg gtaaaatgcc gcaagcgttg
     5041 gggaaactct ggttacttgt cagcatggcg ctcggcgtca ttttcgtctg gatgatgacc
     5101 tgcgtgtatc aaatcgacac cgtgccaacc tggcataacg gttacactac gctggctttc
     5161 ttcctgacgg tattgttgag cggtccaata cttgctgccg caattctgcg ggcagcccgc
     5221 gttacattta ataccacacc atttgccatc attagcgttc tggcgctgat cgcttgtgcc
     5281 gaggtgattg tgctgcaagg tttgtctctg gcgtctattc actcatccgt gcaacaagcc
     5341 agcgcactgg taccagatta cgcctcctta caggtctggc gtgtggtatt gttatgcgca
     5401 ggtcttggat gctggctttg tccgctcatt cgtcgccgtg aacctcacgt tgccggactt
     5461 gttctggggc taatcctcat acttgttggt gagatgatcg ggcgtgtgct cttttatggc
     5521 ctgcacatga cagtaggaat ggctattgca ggttaacaaa ttggtgcggg gcaacccgca
     5581 catttcagga tgttaggaat gacccatttt tcacagcaag ataatttttc tgtcgcggcg
     5641 cgcgtgttgg gcgcgctgtt ttattacgct ccagagagtg cggaagccgc acctctggtt
     5701 gcggtactca ccagtgacgg ctgggaaact cagtggcctc taccagaagc gtcattagcg
     5761 ccgctggtga cggcttttca gacacagtgt gaagagactc acgcccaggc ctggcagcgt
     5821 ttgtttgtcg gcccgtgggc actgccgtct ccgccgtggg ggtcggtctg gctggatcgc
     5881 gaatctgtgc tgtttggcga ttcaacattg gcacttcgtc agtggatgcg cgagaaaggc
     5941 attcagtttg aaatgaagca aaacgaaccg gaagatcatt ttggttcact gctgttgatg
     6001 gctgcgtggt tggcggagaa tggtcgccag acagaatgcg aagaattgct ggcatggcac
     6061 ctttttccgt ggtcaacacg ttttcttgat gtttttatcg aaaaagcaga acaccctttc
     6121 taccgtgcac tgggtgaact ggctcgctta acgctggcgc aatggcaatc acaactgttg
     6181 attcctgtcg cggttaaacc actctttcga taggatcacc gtaatattgc cggatggtga
     6241 cgattacgcg cgattcggca ataatacgtt acaatgtcac gtttttttat ctcttaaagc
     6301 acgcactgct tttgcggttg gcctcttttg ccgcaaaata gtcgcccgtg tttcattgcc
     6361 catttctgct catgcatcat ctacacatct atccggatct gcgcactatg ttccgccgtc
     6421 tgcttatcgc aacagtcgtc ggtattctcg ctgtctttgc cgttgccggg tttcgtcatg
     6481 cgatgctgct actggagtgg ttgttcctca ataatgactc cggcagtctg gtcaatgcag
     6541 cgacaaacct ttcctcctgg cgacggctgc taactccggc gctcggcgga ctggcggcgg
     6601 gtttgttgct gatgggctgg cagaaattta cccaacaacg ccctcatgcg ccgaccgatt
     6661 acatggaagc gttgcaaacc gatggacagt tcgattacgc agcaagcctg gttaaatcgc
     6721 ttgcctcgct gctggtagta accagcggca gtgcaattgg tcgcgaaggt gcgatgattc
     6781 ttttagctgc ccttgccgcc tcctgttttg cccaacgttt tacgccacgc caggaatgga
     6841 aattatggat cgcctgtggg gccgcggcgg gaatggctgc ggcctatcgt gccccgcttg
     6901 ctggcagttt atttatagcc gaagtgctgt ttggcactat gatgttggcc tctctcggcc
     6961 cggtgattat ttccgccatc gtggcatggc tggttagcaa tctgattaat catagcgacg
     7021 cgttactcta caacgtacaa ctctcagtga cggttcaggc tcgtgactat gcgctgatta
     7081 tcagtacagg tgtgctggca ggtctgtgcg gaccactgtt gttaacgtta atgaacgcct
     7141 gtcatcgtgg atttgtgagt ctcaaacttg cgccgccctg gcaactggca ctagtcgggt
     7201 tgatcgtggg tctgctttcc ctgttcacac ctgcagtgtg gggcaacggc tatagcaccg
     7261 tacaatcctt tttaaccgcc ccaccactgt taatgatcat tgccgggatc ttcctttgta
     7321 aactgtgtgc cgtgctggcg agtagcggtt ccggcgcacc cggtggtgtc tttacaccga
     7381 cgctatttat cggtcttgcc attggcatgt tgtatggtcg tagcctggga ttatggttcc
     7441 ctgatggcga agaaattacg cttttactcg gattgaccgg gatggcgaca ctgttggcgg
     7501 caaccacgca cgcgccgatt atgtcgacgt tgatgatatg tgaaatgacc ggggagtatc
     7561 agctactccc cggtttattg attgcctgcg taattgcgtc ggtaatttcg cggacgttac
     7621 accgtgactc tatctaccgc cagcacactg cgcagcatag ctaagcggat gtattgcccc
     7681 agttcacgct gttcggcgcg cggcagataa ggcagttcac caatgagcgg tgccggaagt
     7741 tttttaccca gcacatcaat gatttccgca taatgcgcca gtcctgggtt gattcggtta
     7801 gccacccagc caatgagcgg cagcccgtcg ttggcgatcg cctgagctgt tagcagtgca
     7861 tggttaatgc aaccttcctg aataccgaca accatcaaca ccggcagttg ttcctgcact
     7921 acccattcag agagtggacg caaatcattc atcagactgc gccagccgcc agtcccttct
     7981 accacgacat gatcgacttt atcggtcagg tttgccaggc cgtttgaaat gagggtgtaa
     8041 ttgattgggc aactgtgcgc cacgctactt tcttcttcgc ttaacgcgat aggattaact
     8101 gcttcataag gcagttcgat ggttgaaaca ctctgcaaca ccaaggcatc tttattacgc
     8161 agcccttcgg gtgtctcttt gctccccttc gctacgggtt tatatcccgc aacccttttt
     8221 ccctgggagg ctaacgcttg tagcaatgcg cgggaaacca ccgttttccc tacagaagtg
     8281 tctgtaccgg taataaagaa acgcttcagc atcactaact ccaccgttat gcttcacaaa
     8341 tataaactag gaaaataatt aaccttgaaa gtctaagtta tgctttcctg gcccaaattg
     8401 agatagcgca aattttggta gaacagttaa aaaatgttaa ccctgcaaca gacgaatcaa
     8461 caaagaaccg ttatacatcg cgtcttttac cagtgcagcg cctgccatcg tgccctggtt
     8521 agaaaactga gtactctcaa cgctaatgtg ctgactatac gcaggaaggg cctgctgacg
     8581 gatgctgtct gagatgaccg ggaagaggat atctgccgct ttacttaacg gtgagccaat
     8641 cagtattttt tgtgggttaa ataaattcac catgatggca agaatgcgcc ctacatgcgc
     8701 gcccaccccg ataatgatgt cttttgccag tagatcgccg cgcaatgccg cctgacacaa
     8761 tgagtccacg gttaacggtt gcccatgtaa catcgagctc atggattgat tgagacgcag
     8821 ctgtgccagc tcaagaatac tgtccacact ggcgatggtt tcgaggcagc cgtggttccc
     8881 gcaataacag cgtttaccat atgggtcgac ctgtgtgtgg cctatttcca cgagactact
     8941 gctgcctgcg tgtagcagat gaccatcggt aatgacgccc acccccacgt tgtgatcgat
     9001 aaccacctga atcacatcgc gcgccccgcg tgaggcacca aacaaggcct ctgccatcgt
     9061 ccatgcgctg atatcatgct gaatataaac cggtacgccg gtatgctgct ccagcgcctc
     9121 gccgagcggc atctctttta catcctcgta gaacggcatg cgatgtacaa taccattttc
     9181 cgtatcaata attcccggca gggttatggc aatcgaagtt agacgctcaa gttttttctg
     9241 gtggcggata aaaaactgat cgatatggga aatgatacga tccagcaatg gcaagtcatc
     9301 ttttaacgcc agttcctgcg actcttccac caccagtttg ctgctcagat cgcgcagagc
     9361 aaggaaaatc tccccgcgac taatgcgcag agaaagatag tgccaggctt cagtttcaac
     9421 caccagcccc accgccggac ggccacggtt ccccgcttct ttgatttcca gctctcgcac
     9481 caggtgtgct tcgagcatct cacggacaat tttagtgata ctggcaggag ccagctgcgc
     9541 cagacgggaa agatcgatac gcgagactgg accaagctga tcaatcaggc gataaaccgc
     9601 gcccgcgttg gtctgcttta tttgatcaat gtgcccaggc tggttttcag caaccaccgc
     9661 atactcccta tattttcgcg ctccgaaata atctgtaggc tatggtgaag cacttcaata
     9721 cgtgtcgtca aatttttact taggcatgtg attaacagca catttttcgg gctttttcgc
     9781 tgaaatttcc ctcacctgag agcattcagc agatgaatac gaaagttacg cgcagctgga
     9841 ctttgttcat gatgtttcgg ccagaccaac cacatttcag aaaccgcatc ctcttcagca
     9901 atcggcaccc agcgcatttc gttgagctga acacgtttaa atgacgcagg caaaattgaa
     9961 acacccagac cggcggaaac cagaccgatg atggtcattg cctcgcccac ctcctgagtg
    10021 atgacgggcg tcaaatggta acgtcgcatc agtccgagaa tatcgtcata cagcccagtc
    10081 ccgacgtgcg gatcaaaaaa gacaaagggt tctttcgcca gttcagccag ttcagccagt
    10141 gttacactcg gattatttgc caggggatga tcgtacggga tcatcgccat aagcggttca
    10201 tggacgatga ctgcgtgttc aagcgtctcc ggtaacgctg tgttacgcag caatcccata
    10261 tccagcgttc cttcaatgag cggagcgatt tgctcgcgag tgttcatttc gcgggtttgt
    10321 aaatggacat caggataatc acggcgaaac agcgataacg tatcggacac cgcccgaata
    10381 aaaggagcgg acgaagtaaa accaatacgc aactcccccg cttcaccctg atgcagtctt
    10441 tcagcgcgag cggcagcgtc atccaccata gacaggattt gccgactatc tgcaagaaac
    10501 tgttttcctg ctgccgtcag caatacactg cgattagttc gtgccagcag tcgggcaccg
    10561 atttgttgct ccagtgcctg aatctgctga cttagcggcg gttgcgaaat attcaggcgg
    10621 gcagccgcgc gcccaaaatg cagctcttcc gcaacagcaa caaagtaacg cagatgacga
    10681 agttcaatat tcatatttaa aacatcttat ttgagattat taatatatta gacagaacaa
    10741 ttcgattttc ctaccctatg tataagcctg atccacaggc atatttagca aggatttcaa
    10801 gtgagccgta ctacaactgt tgatggcgct ccggcaagcg acactgacaa gcaaagcatt
    10861 tctcagccaa atcaatttat taaacgcggt acgccgcaat ttatgcgcgt caccctggcg
    10921 ctgttctctg ccggactggc aacctttgca cttctctatt gtgtgcagcc tatccttccg
    10981 gtgctttcgc aggagtttgg cttaacgccc gcgaacagta gtatttcact gtccatttcc
    11041 acggcgatgt tggctatcgg tttgctattt actggcccgc tatccgatgc cattggtcgc
    11101 aaaccagtga tggtcacggc gctactgttg gcctccattt gtacgttact ttcgacaatg
    11161 atgaccagct ggcacggcat tttgattatg cgcgccttga ttgggctttc gttaagtggc
    11221 gtggcagctg ttggcatgac ttatcttagc gaggaaatcc accccagttt cgtggccttt
    11281 tcgatggggt tgtatatcag cggcaactca attggcggca tgagcggacg cttaattagt
    11341 ggtgtcttca cggacttttt caactggcga attgctctgg cggcaatcgg ttgtttcgcg
    11401 ctggcctcgg cgttgatgtt ctggaaaatc ctccctgaat cacgccattt ttgcccgact
    11461 tcgctgcgcc ctaagacgtt atttatcaac tttcgtctgc actggcgtga ccggggatta
    11521 ccgttattgt tcgcagaagg ctttttgctg atggggtcgt tcgtcacgct gtttaattac
    11581 atcggctatc ggttgatgct ttccccctag catgtcagtc aggccgtggt tggcttatta
    11641 tcgctggctt atttgaccgg tacatggagc tcacccaaag ccggaaccat gaccacccgc
    11701 tatgggcgcg gtccggtgat gttgttttcg acgggagtta tgccgtttgg tttactgatg
    11761 accttattta gctcgctgtg gctgatcttt gccggaatgt tactcttctc agccggattc
    11821 ttcgcagccc actcagtagc cagcagctgg atcggccccc gcgcaaaacg cgctaaaggc
    11881 taggcctcct cgctgtatct gttcagttac tatctggggt cgagtattgc cgggacgctg
    11941 ggtggtgttt tctggcataa ctatggctgg aacggcgtcg gcgcatttat tgctctgatg
    12001 ctggtcattg ctctgctggt cgggacgcgt ttgcatcgtc gtctgcacgc ctgaaaaata
    12061 agtccggact gcggtaaata cccgtccgga cttattgcct gctcaaacca acgttaatag
    12121 ccatcctaaa atagacgaag cgccagccaa ttcccgcagc gcatctagcg tcatcaggat
    12181 tataagtacc caaataaacg gattcatttt gctgtgtgtc atttattact gatgcgcagt
    12241 tattctaccg ctttgtaagt agtataatag ttaacccgat caagactact attattggta
    12301 gctaaatttt ccttaagtca caatacgtta ttatcaacgc tgtaatttat tcagcgtttg
    12361 tacatatcgt tacacgctga aaccaaccac tcacggaagt ctgccattcc cagggatata
    12421 gttatttcaa cggccccgca gtggggttaa atgaaaaaac aaattgaggg tatgacaatg
    12481 aaaaaagtat tagctctggt tgttgccgct gctatgggtc tgtcttctgc cgcctttgct
    12541 gcagagactg cgaccacacc tgctccgact gcgacgacca ccaaagcagc gccagcgaaa
    12601 actacacatc ataaaaaaca gcataaagca gcacctgccc agaaagcgca ggcggctaaa
    12661 aagcatcata aaaatacgaa agctgaacag aaagcccctg aacaaaaagc gcaggcagcg
    12721 aagaaacacg ccgggaaaca cggccatcag caaccggcaa aacctgctgc acaacccgca
    12781 gcgtaagttt tcaacagtaa tgctggcgcg ccccctcgcg cctgaaaatt acggtgctaa
    12841 gcgggtaacg tttagcaccg cctttagcgg agggtagtat ggtgggccgt tatcgctttg
    12901 agttcattct tatcatcctt attttatgcg cactgattgc cgcccgtttt tatctttcct
    12961 gactgtagtt atctgatttt actcccactt tcatcccgtc ccgtctatag tatttatgag
    13021 ggtttgcttt taataatcat aattacccac cagagtgtga tatgcgtaca accattgctg
    13081 tagtgttggg tgcaattagt ttgacgtctg cttttgtgtt tgcagataaa ccagacgttg
    13141 ccaaatcggc aaacgatgag gtcagcaccc tgttttttgg tcatgatgat cgtgtgccag
    13201 tgaatgacac gacccaatca ccgtgggatg cggttgggca actggaaacg gccagcggca
    13261 atttatgtac ggcgacgctg attgcaccca atctggcatt aacggcagga cactgtttat
    13321 tgacgccgcc taagggtaaa gcggataagg cggtagcgct gcgttttgtg tcaaataaag
    13381 gtctttggcg ctatgagatc cacgacatag aaggccgcgt tgatccgaca ctgggaaagc
    13441 ggttaaaagc agatggggat ggttggattg ttcctcccgc agctgcaccg tgggacttcg
    13501 gattgattgt gctacgtaat cccccttctg gcattacacc gttgccgtta tttgagggag
    13561 ataaagccgc gcttactgcc gcattaaaat cggctggtcg taaagtgact caggcgggat
    13621 accctgaaga tcatctcgat acgttataca gtcatcaaaa ctgtgaagtg actggctggg
    13681 cgcaaacgtc ggtgatgtca catcagtgcg ataccttgcc gggtgacagc ggttcgcctc
    13741 tgatgttgca taccgatgac ggctggcaat taattggggt gcaaagttcg gctcctgccg
    13801 cgaaagatcg ctggcgcgcc gataaccggg ccatttctgt taccggtttt cgcgacaagc
    13861 tggatcaact gtcgcaaaaa taatgttcaa acgctgcccg acagcgcggg cagcgtcttc
    13921 atcaggcaag tttcaccatg atcattccag ccaacagcaa gaccaggcca atccagcctt
    13981 tacgatttaa ccgttgacca aacaagatcc aacctgcggc taacgtggcg gcaataccaa
    14041 acccgcccca caatgcataa acgacagaca agtcgatccc tttaacggcc tgagaaagcg
    14101 cactaaaggc cgccagcacc gccgccaggg agagcaagcc aaatattttg cgacgaaagc
    14161 cgtcagaaaa tttcaaaaag acgttagcaa cgatttccag cacgattgcc aatgccagcc
    14221 aggcggcgtg aacccattca aactgcgcca tggttcacct ccagttcagg tttacgcgct
    14281 ttacgggtac ctgatttgat caacactatc ccggcgacca gggtggttaa cccggcaatt
    14341 ttcatcagcg ataaactttc gtcgaataac aaaacgctaa acaaggtaat aaataaaata
    14401 ccgatacctt cccacagcgc ataagctacg cctaaggcga tttttttaac ggcgaaagag
    14461 agaaatatat acgacagaga aatcatcacc agcattaaaa taaagccgcc atttccctca
    14521 ctgacgctcg cccatttcat tgacagcgta ccggtaattt ctgtagcaat agccagacct
    14581 aataaaatcc aataaatata cattgtcctt ctcctgcaag agaactattt taattttcgc
    14641 ttaattcagc gaaaccaaag taaagtggtc aagccctgcg catagggcaa gcttaggcag
    14701 aagaaaggac taaagcgcgt tgcgccagtg ctgctcacct acgagcaaga tagtggatga
    14761 ggtacgaaga tgggtaaatg tagaaaataa cgtcctgaac aaattgtcca taatattaca
    14821 attatccgca gtgttgcttc tcgtcatcgc agatgataat tgtcctcggt agttgaacac
    14881 gcctgatttg tatcatagct taagaattaa ctcaaaatat tttcacttct ttacctgagc
    14941 ggtttgattt tcgttatgat gacggagcga aaaagacatt attattagca aaggaagaaa
    15001 aacggggaca agcatggcaa agccgatcat cacgctcaat ggcctaaaaa tcgtcattat
    15061 gttgggaatg ctggtcatta ttctctgcgg tatccgtttt gccgccgaga tcatcgtgcc
    15121 gtttattctc gcattattta ttgctgttat tcttaacccg ctggtgcaac atatggtccg
    15181 ctggcgtgtg ccacgtgtac tggcagtgtc gattttgatg acaatcatcg tgatggcgat
    15241 ggtgttgcta ttagcttatc tgggttccgc gctcaacgag ttgacgtgga cgttaccgca
    15301 atatcgcaac tctattatga cgccgctgca agctcttgag ccgttgttgc aacgcgtagg
    15361 gattgacgtc tcagttgacc aactggcgca ttacattgat ccgaacgcgg cgatgacgtt
    15421 gctcaccaac ttattgacgc agttatctaa tgccatgtca tcaatatttt tattgctgct
    15481 gacggtgctg tttatgctgc tcgaagtgcc acaattgccc ggaaaatttc agcaaatgat
    15541 ggcgcgtccg gttgaaggga tggcggcgat tcaacgtgcg attgacagtg tttctcatta
    15601 tctggtgctg aaaacagcca tcagcatcat caccggcctg gtcgcctggg cgatgctcgc
    15661 cgcactcgat gttcgcttcg cttttgtctg gggattgctg gcctttgcgc ttaattacat
    15721 ccctaatatt ggttcagttc tcgcggcaat cccccctatc gctcaggtac tggtgtttaa
    15781 tggcttctac gaagcgttgc tggtgctggc gggatatctg ctgattaatc tggtcttcgg
    15841 caatattctg gagccgcgca tcatgggacg tgggctgggg ctttcgacat tggtggtatt
    15901 tttgtcgttg attttttggg gatggttgtt aggaccggtg ggtatgctgc tttccgtgcc
    15961 gttgacaatt attgtcaaaa ttgcgcttga acaaacagcg ggaggtcaaa gcatcgccgt
    16021 tctgttaagc gatctcaata aagagtgacg gcctccgcag aggccgtcag ggttacagag
    16081 ctttcaggat tgcatccacg ctggctttgg cgtcaccaaa cagcatgtgg gtgttttcct
    16141 tgaagaacag cgggttttgc acaccagcat agccagtgtt catcgaacgt ttaaagacaa
    16201 tcacgttctg cgctttccac acttccagca caggcatacc agcaatcgga ctcttcggat
    16261 catcctgcgc cgccgggtta accgtatcgt tagcaccaat caccagtacg gtatcggtat
    16321 cagcaaagtc atcgttgatc tcgtccattt ccagcacgat gtcatacggt acttttgctt
    16381 cagccagcaa tacgttcata tgtccaggca aacgccccgc aaccgggtgg ataccgaaac
    16441 gcacattaat accacgagcg cggagtttct cggtaatttc agcgacagga tattgcgcct
    16501 gcgcgactgc catgccgtac cccggagtaa tgatcactga atgggagttt ttcagcagtt
    16561 ccgctgtctc ttctgcggtg atttcgcggt gctcacccac ttcctgatca tcgccagtag
    16621 aagagccgtc ggtgccgaaa ccacccgcaa taacgctgat aaaggaacgg ttcatcgcct
    16681 tacacataat gtaagaaagg atagcccccg aagaaccgac cagcgcaccg gtcacaatca
    16741 gcaggtcgtt gctgagcata aagcccgcag ccgcagccgc ccagccggag tacgagttca
    16801 gcatcgacac caccaccggc atatctgcac caccgatgga ggcgactaaa tgccagccga
    16861 ataccagcgc aatggcggtc attatcagca atgccagcac ttgcaggccg acgctgtccg
    16921 tgcgaacaaa tacaatcagc agcaagaagg aaacgaccag agccgccagg ttcattttgt
    16981 gacggtttgg cagcatcaac ggtttagacg aaatcttgcc acacagtttg ccgaacgcca
    17041 ccaccgagcc cgtgaacgtt accgccccga tgaagatacc gaggaacact tccgtcaggt
    17101 gaatattgac cagaatcggt gccattcccg cgtcatgatg cagataactg ttaaagccaa
    17161 ccagcactgc cgccagaccc acgaagctgt gcaggatcgc caccagttct ggcatttcgg
    17221 tcatttcaac tttcttcgcc agccggatac caattgcccc accaatgacc atcgccagca
    17281 agatccagcc aacattaccc gtatccggtc caaaaatggt tgcgattaac gcaatcgcca
    17341 tcccggcgat accgaagttg ttaccctggc gagacgtttc atgtttcgaa agaccggcca
    17401 gactgaagat aaacaggatc gcggcaacaa tgtatgcagc tgtaactaat cctccagaca
    17461 tatgttaccc cttaattttt gcggaacatt ttcagcatgc gctgagtcac ggtgaagcca
    17521 ccgaaaatat taatgctggc tataagcacc gcgataaaac taaggaagct aacccagcct
    17581 ccctggccaa tctgcaacag tgctccgaca acaataatcc ctgaaatcgc gttggtaacc
    17641 gacatcaacg gtgtatgcag cgcgtgcgat acattccaca ccacgtaata accgacaacg
    17701 caggccagcg cgaaaacggt gaagtgccca agaaactctt tcggcgcaac gcttgacagc
    17761 caaccgaaca ggatgattgc cagcgccatc aacgcgtatt tacgccacgg tgagcaggca
    17821 catttttcct cagttttcac ttccggtgcc gctttttgtg ccgcctgcgg ctgagctgat
    17881 acctgaatcg gcggtgccgg ccaggtaatt tcgcccgcac ggatcacggt cacgccgcga
    17941 atcaccacat catcaaaatc aacagtgata ttgccgtctt tctctttgca caacagtttc
    18001 agcagattaa cgaggttagt accgtaaagc tgtgaggatt gcgtcggcag acggcccgga
    18061 agatcggtat aaccaatcac tttgacacca ttttccgtac tgaagatttc acccggcacg
    18121 atgtattcac agttgccacc gttttgggct gccaggtcga caatcacact gcccgccttc
    18181 atggagtcaa ccatttcacg ggtaattagc ttcggcgctg gtttgcctgg aataagcgcg
    18241 gtggtgacaa tgatatcgac ctcttttgcc tgggcggcaa agagttccat ttccgctttg
    18301 atgaacgcgt ccgacatcac tttggcatag ccatcgccgc tgcccgcttc ctctttaaaa
    18361 tccagctcga ggaattccgc gcccatactt tgaacttgtt ctttcacttc cgggcgggtg
    18421 tcgaatgcac gcacaatcgc gccgagactg tttgctgcgc caatggcggc caaacctgca
    18481 acaccggcac caatcaccat cacttttgcc ggtggcactt tcccggccgc agtgatttgc
    18541 ccggtaaaga agcgcccaaa ttcatgtgcc gcttcaacaa tggcgcgata accggcgata
    18601 ttcgccatcg agcttagtgc gtccagcgat tgtgcgcgtg agatacgcgg cacagagtcc
    18661 atcgccatca cggtcacgtt acgttccgca agtttttgca ttaattccgg attctgcgca
    18721 ggccagataa aactcaccag cgttgtcccg ggattcagta acgcaatttc atcatctaac
    18781 ggcgcattga ccttcagaat gatctctgac tgccagacgc tattcccttc tacaatttca
    18841 gcgcccgctt gcacaaacgc tttatcgtca aaacttgcca gttgacccgc gccgctctct
    18901 accgcgacgg taaaacccag tttcagcagc tgttccactg tttttggcgt tgctgcaaca
    18961 cgggtttcat tggttaaccg ttctcttggt atgccaattc gcatgatatt cccttccatc
    19021 ggttttattg atgatggttt gcctgtgtca ggagccacac aagctgctca tgtacgagct
    19081 aaatgttact ccgttaaaat aaattagtaa caaacgccct ataacgtact gaaaattatg
    19141 cctgtgatct agcgccaaaa atcagtattt cggcgtgaac tcgcaaaata ttaacgattc
    19201 agccgtgata gtgggataaa caccttagaa cgccggataa agactgataa ttgtcttcga
    19261 cggtcgggta aaacgagaca tcgccccggc acgaatcact acttaacatt aaattaactt
    19321 atacaattca gttgcttcag tagtaatgat gctgatacgg ctgtttttta agcatagacg
    19381 gtcatttgag caggattaaa attggtttaa ggaatgtgat atgaaaaatg acgcagacag
    19441 ttacaccgtt taaatgcaat aatcagccac gtttctcgtt aataacaata ccagtacctg
    19501 gtttgcgcaa ggcgaaggat tatttttatg aagcttaaga acaccctcct ggcgtcggca
    19561 ctgctttctg ctacggcatt ctccgttaac gcagcaacag aactgacacc ggagcaagcg
    19621 gcagcggtta aaccttttga ccgtgtagtg gttaccggtc gttttaatgc tattggcgaa
    19681 gcggtgaaag ccgtttctcg tcgcgcagat aaagaaggtg ccgcctcttt ttatgttgtc
    19741 gacacttctg attttggtaa cagcggtaac tggcgtgtgg tcgctgacct ctataaagcc
    19801 gatgctgaaa aagcagaaga aacaagtaat cgcgtaatta acggtgttgt cgaactgccg
    19861 aaagatcagg ctgttctgat tgaaccgttt gacacggtca ccgtccaggg cttctatcgt
    19921 agccagccag aagtcaatga tgccatcacc aaagcggcaa aagcgaaagg tgcctactct
    19981 ttctacatcg ttcgtcaaat cgatgccaac cagggcggca accagcgtat tactgcattc
    20041 atctataaaa aagatgctaa gaaacgtatc gtccagagcc cggatgtgat cccggcagat
    20101 tccgaagcag gacgtgcagc tctggctgcc ggtggcgaag ccgcgaagaa agttgagatc
    20161 ccgggtgttg cgactaccgc atcaccaagt tctgaagtcg gtcgcttctt tgaaacccag
    20221 tcatcaaaag gcgggcgtta caccgtcacg ctcccggatg gcactaaagt cgaagaactg
    20281 aacaaagcga cagcagcgat gatggtcccg ttcgacagca tcaaattctc tggcaactac
    20341 ggcaacatga ccgaagtctc ctatcaggtt gcgaaacgtg ccgcgaagaa aggtgctaag
    20401 tattaccaca tcacccgcca gtggcagaaa cgtggtaata acctgaccgt cagcgcagat
    20461 ctgtataaat aactattcag ttactcctat aaggcggctt gatgccgcct ttcgcatttt
    20521 tagtcacaaa tttccccgcg accattgcat gcagacacat ctcttcgtaa aatcccgcgc
    20581 ttcagtggca aactccattt ttatgcgttt tgccctaata tttattcttc ttatcacgtt
    20641 ttaatcactg gatatcgatg gaaaagaaac tgggactgag cgcactcacc gcgctggtat
    20701 taagctcaat gctgggcgcg ggtgttttca gtctgccgca aaatatggcg gcagttgcca
    20761 gcccggcagc actgctcatc ggctggggta ttactggcgc tggcatttta ttgctggcct
    20821 ttgccatgct gatcctcacg cgcattcgtc ccgaacttga tggcggtatc tttacctatg
    20881 cccgcgaagg gtttggcgag ctaatcggct tttgttccgc atggggatac tggctgtgcg
    20941 cggtcatcgc caacgtctcc tatctggtga tcgttttttc cgcgttaagc ttttttacgg
    21001 acacgccgga attgcgcctg tttggcgatg gtaatacctg gcaatcgata gtcggtgcat
    21061 cggctttatt gtggattgtt cactttttga tcctgcgcgg ggtgcaaacc gctgccagca
    21121 ttaacctggt ggcgacactg gcaaaattgt tgccgctggg tctgtttgtt gtgctggcga
    21181 tgatgatgtt caaactggat accttcaagc tcgacttcac cggacttgca cttggcgtac
    21241 ctgtttggga acaggtgaaa aacaccatgc tgatcaccct gtgggttttc attggtgtgg
    21301 aaggtgccgt ggttgtttct gcgcgtgcgc gtaataaacg tgatgttggc aaagcgacac
    21361 tgctggcggt tctctccgct ctgggcgttt acttgctggt aacgctgctt tcgctgggtg
    21421 tggtggcacg tcctgaactg gctgaaattc gtaacccgtc aatggccggt ctgatggtgg
    21481 aaatgatggg accatggggc gaaatcatca tcgctgccgg tttgatcgtt tccgtttgcg
    21541 gcgcgtactt aagctggacc atcatggcgg cagaagttcc gttcctggct gccactcata
    21601 aagcattccc acgcattttt gcgcgccaga acgcacaagc tgcgccatcc gcctcactgt
    21661 ggttgaccaa catctgcgtg caaatttgtc tggtactcat ctggctgacc ggttccgatt
    21721 acaacacgtt gctgaccatc gcctcagaaa tgattctggt gccctatttc ctggtcggcg
    21781 cgttcttgct gaaaatcgca acccgtccac tgcataaagc ggtaggtgtc ggtgcctgca
    21841 tttatggctt atggttattg tacgcctctg gtccaatgca cttgctgtta tccgttgttc
    21901 tgtatgcacc ggggctgctg gttttcctgt atgcgcgcaa aacgcataca catgataatg
    21961 tactgaaccg tcaggaaatg gtgctgattg gcatgctgtt aattgcctca gttccggcga
    22021 cctggatgct ggtgggataa cgttcatccc atcgttggat agcaaaggag ataacgatgg
    22081 gtaaaaccca gcccttgcca atattaatta ctggcggagg tcgtcgcatc ggcctcgccc
    22141 tcgcatggca tttcattaat caaaagcaac cggtgattgt cagctatcgg acacactatc
    22201 cagccattga tggactgatt aatgcaggtg cgcagtgtat tcaggctgat ttttcgacca
    22261 acgacggtgt gatggcgttt gccgatgaag tactaaaaac cacccatggt ctgcgtgcta
    22321 ttttgcataa cgccagtgcg tggattgcgg aaaaaccggg tgcgccactg gccgacgtac
    22381 tggcttgcat gatgcagatc cacgttaata ccccatacct gctcaaccat gcgctggaaa
    22441 gattactgcg tgggcacgga cacgccgcca gcgatatcat tcactttacc gattatgtgg
    22501 tggagcgcgg tagcgacaaa catgttgcgt atgctgcaag caaagcggca ctggataata
    22561 tgacccgctc gtttgcccgc aagctggcac cggaagtgaa agtgaattct attgcgccat
    22621 cgctgatcct gtttaatgaa catgatgatg ccgaatatcg acaacaggcg ctgaataaat
    22681 cactgatgaa aaccgcgcct ggcgagaaag aagtgatcga cctggtcgat tacttactta
    22741 ccagttgctt tgtcaccgga cgcagtttcc cacttgatgg cggtcgtcat ctgcgttaat
    22801 gcagctttat ccagcatata atcaacaccc atgcgcttat cccccaacac gcaacagaac
    22861 caacaaaagc ggcgggcaag cgcatcattc cggtgaaata ccacagcgac accaggtaaa
    22921 caaaataggg aataatcgac cacatactaa aaatgatggt tgcgcgtaag gcttcaatgc
    22981 cgcgttcgct ggcaacaata taatgcgcga taagcgcaaa ggtcgggaaa agtggaatca
    23041 gcccggcgat ataataattt ttcgtttttg ctaaaacacc aatcaacagc actaccagcg
    23101 caccgagcgc ggctttgatt accagcccca tctttttacc ttaacacttc cataacaagt
    23161 catcagtaga atacctgatg aaaacttgtt tagaaacgat tgatagtaag taaaaacagc
    23221 gcggtgtatt gtgacgtttt tatatctacc gtgaatgtta tgaacactat cgtatttgtg
    23281 gaagatgatg cggaagtcgg ttcactgatt gccgcgtacc tggcaaaaca tgatatgcag
    23341 gttaccgtag aaccgcgcgg cgaccaggcc gaagaaacca ttttgcgaga aaatccggat
    23401 ttggtgttac tcgacatcat gctaccaggc aaggacggca tgaccatttg tcgtgattta
    23461 cgcgcaaagt ggtctggacc gattgttctt ctaacctctc tcgatagcga tatgaaccac
    23521 atcctggcac tggaaatggg tgcctgcgac tatattctca aaacgacgcc ccctgctgtt
    23581 ttgctagcgc gtttacgttt gcatttgcgt cagaatgagc aagccacact gaccaaaggt
    23641 cttcaggaaa cgtctcttac tccctacaaa gccctgcatt tcggcacgtt aactatcgac
    23701 cccatcaacc gcgtagtcac cctggctaac actgaaatct cgctctcgac agctgatttc
    23761 gaattattgt gggaactagc tacgcatgcc gggcaaatca tggaccgcga tgcattgctg
    23821 aaaaatttac gcggcgtcag ttatgacgga ctggatcgta gcgtggacgt ggctatttcg
    23881 cggttaagaa aaaaactgct cgataacgcc gcagaacctt atcgcattaa aactgtgcgt
    23941 aacaaaggct atctttttgc gcctcatgca tgggatagtg acaataaaca atcagatgac
    24001 taatatttgt tcatctgaaa aagagaacaa ttaacgaatt caattaataa gagcttattc
    24061 aaattccaaa ccccctacat ttgatatgaa aatgagaaaa aatttctcta taatcgcctc
    24121 gtccctgcgt cttatggcgc agatttttta caacaaactc tttccggcat ttcaggtgct
    24181 gaatgaggca agaggcttaa aaaacggtta caactttcac atatattgtg ctttttttga
    24241 tctgctcatg ggggattttc aaaacggatc aaaagagcga atccctttcc aaaagtgttt
    24301 ttatcgcgat tttcctgggg gctatcattg gtatctccct gcatttcatt cctgagacaa
    24361 gcagtgaaac cgttatcgaa tggtataata ttgctggtaa cggttatgtt aatttattaa
    24421 aacttgttgc cgttccactc atattcattt cgattctttc agcgattaat aagctcgaaa
    24481 at
//
LOCUS       DAEEUE010000057        24450 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.057, whole genome shotgun sequence.
ACCESSION   DAEEUE010000057 DAEEUE010000000
VERSION     DAEEUE010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24450
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.057"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..1014
                     /locus_tag="KLK84_002448"
     CDS             <1..1014
                     /locus_tag="KLK84_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
                     /protein_id="HBE2009737.1"
                     /translation="NEQATQELASTSTRLSGGMYADAHAEWGVKPKILGVGGGVGIRG
                     GFKGSLDATDDDSHSASSGSRATHDARHDIDAKASKDFKEASDYFTSRKVSESGSHTD
                     NNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEYAEMASRTESMSGQMSEDLSQQFAQ
                     YVMKRAPQDAEAILTNTSSPEIAERRRAMAWSFVQEQVQPGVDNAWRESRGDIGKGME
                     SVPSGGGSQDIIADHQGHQAIIDQRTQDSNIRNDVKHQVDNMVTEYKGNIGDTQNSIH
                     GEENIVDRQYSELKNNHKQEEIQQNNRYNEENKRQILMPTPSEDALKQMVDDKKERLK
                     GPL"
     gene            1011..1520
                     /gene="traS"
                     /locus_tag="KLK84_002449"
     CDS             1011..1520
                     /gene="traS"
                     /locus_tag="KLK84_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898910.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="HBE2009738.1"
                     /translation="MITHYDIKMEMQKLKEVLSVEGVNIPSLLQVIKPGTYVFLWVLL
                     WPTFLRLVSVKSDVRDVGFDICASGMMGFLLFVAITNGMMLYLAIPDSFRKDSKIINF
                     MYSKSKTYILLFLIVFSMVSFMHSILYVFALMITFILFFLVYTIDINRYNLSAIASVI
                     GLFKKESVS"
     gene            1534..2265
                     /locus_tag="KLK84_002450"
     CDS             1534..2265
                     /locus_tag="KLK84_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HBE2009739.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            2468..3205
                     /locus_tag="KLK84_002451"
     CDS             2468..3205
                     /locus_tag="KLK84_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009740.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILTKISFPEKSGSNLLKPEAVSC
                     NLLSLCVLAPVFCTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQAENITWAISSQS
                     PADKYDLINKTSCVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRTAGGHHHRHTGYTWSVSVN"
     gene            3256..5472
                     /gene="traD"
                     /locus_tag="KLK84_002452"
     CDS             3256..5472
                     /gene="traD"
                     /locus_tag="KLK84_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HBE2009741.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLV
                     LWVKISWQTFINGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGHQGKQQSENEVTGGRQLTDNPKEVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEGVTQAEQPQQPQQ
                     PQQPQQPQQPQQPQQPQQPQQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQ
                     LPPGISESGEVVDMAAYEAWQQENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            5472..10742
                     /gene="traI"
                     /locus_tag="KLK84_002453"
     CDS             5472..10742
                     /gene="traI"
                     /locus_tag="KLK84_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HBE2009742.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHV
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAE
                     WMQTLKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLQEGMVFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYDRLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETSLETAISLQ
                     KTGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPASERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGETFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISKDDGLITLEDAEGNTRLIS
                     PREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAQRLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTELAVRDIAGQERDRSAISERETALPESVLRESQREREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            10762..11508
                     /gene="traX"
                     /locus_tag="KLK84_002454"
     CDS             10762..11508
                     /gene="traX"
                     /locus_tag="KLK84_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HBE2009743.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSYRLYRAEDRAERLALLACLLAVIPALNLASSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            11567..12427
                     /locus_tag="KLK84_002455"
     CDS             11567..12427
                     /locus_tag="KLK84_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBE2009744.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDIISVINWAEKQECIDNQRIG
                     LWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGDVLVTGEMNESERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDNESKVFFEKVKGQYPEMDIKIPFLTVMETLQYKPAESAA
                     KVQCPVLIVIAGQDSVNPPEQGKALYDAVASGTKELYEEADACHYDIYEGAFFERVAA
                     VQTQWFKKHL"
     gene            12530..13090
                     /locus_tag="KLK84_002456"
     CDS             12530..13090
                     /locus_tag="KLK84_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HBE2009745.1"
                     /translation="MTEQKRPVLTLKRKTEGETPTRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELTAKKAQARQALSIYLNLPSLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEV
                     YAAERLDKIRRQNRIKAELQAVLDEQ"
     gene            13219..13431
                     /locus_tag="KLK84_002457"
     CDS             13219..13431
                     /locus_tag="KLK84_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HBE2009746.1"
                     /translation="MNGTGRDSQNSGVWRYRQVGRRAVRLEVRGDWPGAAEAWLRAAQ
                     SAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            13676..14137
                     /locus_tag="KLK84_002458"
     CDS             13676..14137
                     /locus_tag="KLK84_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HBE2009747.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLINIDAPEKKQDYGRWSTEMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            14183..14392
                     /gene="hha"
                     /locus_tag="KLK84_002459"
     CDS             14183..14392
                     /gene="hha"
                     /locus_tag="KLK84_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HBE2009748.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSTSERESFNSAA
                     DHRLAELITGKLYGRIPKEIWKYVR"
     gene            14431..15021
                     /locus_tag="KLK84_002460"
     CDS             14431..15021
                     /locus_tag="KLK84_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HBE2009749.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGAAQQSPEHRS"
     gene            15261..15509
                     /gene="repA"
                     /locus_tag="KLK84_002461"
     CDS             15261..15509
                     /gene="repA"
                     /locus_tag="KLK84_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HBE2009750.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKEL
                     RVFIPAALKAQLQVMCEAEGVTQAEMIAELIKQKSAFS"
     gene            15754..15828
                     /gene="tap"
                     /locus_tag="KLK84_002462"
     CDS             15754..15828
                     /gene="tap"
                     /locus_tag="KLK84_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HBE2009751.1"
                     /translation="MPGKVQDFFLCSLLLCNISAGRCD"
     gene            15821..16678
                     /gene="repA"
                     /locus_tag="KLK84_002463"
     CDS             15821..16678
                     /gene="repA"
                     /locus_tag="KLK84_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HBE2009752.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESGAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVAAARRSRVEWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDANRERQDIVTLVKRQLTREISEGRFTANGEAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            17380..17640
                     /locus_tag="KLK84_002464"
     CDS             17380..17640
                     /locus_tag="KLK84_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009753.1"
                     /translation="MAENGYGLAGQGMGKVKSINQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGISICWWLCLKGYILYIKPDNTQIARQMSIMHPVIACL"
     gene            17747..18010
                     /locus_tag="KLK84_002465"
     CDS             17747..18010
                     /locus_tag="KLK84_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HBE2009754.1"
                     /translation="MNSTTHYENANFLRELAESLPRIFPEGSTDKSALLQRLANEELA
                     RAEYDEQIRAKVAAARADKRPGMSSAQLRQQLQGRYQELRNEL"
     gene            18000..18299
                     /locus_tag="KLK84_002466"
     CDS             18000..18299
                     /locus_tag="KLK84_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBE2009755.1"
                     /translation="MSYEIEWRPGAIEDITALFEYLVENASLWDARNVTERLITATDR
                     LADYPRLYETDERYGEGVRRISLLGQHVLYEVNDEASRVNILAVVGQRQNPRFIL"
     gene            complement(18335..19000)
                     /locus_tag="KLK84_002467"
     CDS             complement(18335..19000)
                     /locus_tag="KLK84_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009756.1"
                     /translation="MMFWNRNKKDKATIANEKKRFNHLLSVAEKLPVKALPDLIRAIV
                     RPVQSDFLLAVAEEGTDARPDMTPQEFFFKGLTQIQSYEKMKECEMNGADYPLSLASD
                     IVLPWPWSLPRYIDNVSRIGTHKGKPWKQDKNNHYVDLWLPWRIGFVHGGNHSITAGI
                     LAGEGSLIPEHVYDMSWMFELVRTDGNNWFVEGQKAEAVKSGRSAAVFEIGRLLVEGA
                     KER"
     gene            complement(19570..20133)
                     /locus_tag="KLK84_002468"
     CDS             complement(19570..20133)
                     /locus_tag="KLK84_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBE2009757.1"
                     /translation="MDIIEKRITKRHLSESELSGVNYYNCIFERIQLDNFNFRDCEFE
                     KCRFVNCSIKNLKLNFFKLIDCEFKDCLLQGVNAADIMFPCTFSLVNCDLRFVDFISL
                     RLQKSIFLSSRFRDCLFEETDLRKSDFTGSEFNNTEFRHSDLSHCDFSMTEGLDINPE
                     INRILSIKIPQEAGLKILKRMGVVVGG"
     gene            complement(20137..20868)
                     /locus_tag="KLK84_002469"
     CDS             complement(20137..20868)
                     /locus_tag="KLK84_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBE2009758.1"
                     /translation="MTIPLLEINSLSFSYKVNLPPVFNNLSLKIEQGELIGLLGENGA
                     GKTTLFNLIRGGVSNYEGTLKRNFSGGELVSLPQVINLSGTLRNEEVLDLICCFNKLT
                     KKQAWTELNHKWNDNFFIRYDKIRRKRTYTVSYGEKRWLIISLMLTLCKNARLFLLDE
                     PTVGIDIQYRMMLWELINKITADGKTVFFSTHIFDELTRDKIPFYMLSKNSINRYSDM
                     SDFIQSNNETTPEKAFIKEVMGTGD"
     gene            complement(20865..21590)
                     /gene="mcbE"
                     /locus_tag="KLK84_002470"
     CDS             complement(20865..21590)
                     /gene="mcbE"
                     /locus_tag="KLK84_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin B17 immunity protein McbE"
                     /protein_id="HBE2009759.1"
                     /translation="MVTLKMAIIFLMEQIRTPFSLIWTIMSPTVLFFFLHFNEIELHY
                     GDTAWLGKQISWFVGYISFSVVLFNYCLYLVGRRESGFIATFVHNMDGRLLFIRSQLI
                     ASLIMSILYVFFFILVVLTGFQASPDYQIVMIILKSIYINAFMMVSLTFMASFRVTFQ
                     TASTIYSVLITVCMVSGIVSLKYNEGIVYWINQVNPIAIYSTILQSDQELSLMTIFFY
                     SIMLIISIISALTFKTEPVWSSQ"
     gene            complement(21600..22790)
                     /locus_tag="KLK84_002471"
     CDS             complement(21600..22790)
                     /locus_tag="KLK84_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HBE2009760.1"
                     /translation="MINVYSNLMSAWPATMAMSPKLNRNMPTFSQIWDYERITPASAA
                     GETLKSIQGAIGEYFERRHFFNEIVTGGQKTLYEMIPPSAAKAFTEAFFQISSLTRDE
                     IITHKFKTVRAFNLFSLEQQEIPAVIIALDNITAADDLKFYPDRDTCGCSFHGSLNDA
                     IEGSLCEFMERQSLLLYWLQGKANTEISSEIVTGINHIDEILLALRSEGDIRIFDITL
                     PGAPGHAVLTLYGTKNKISRIKYSTGLSYANSLKKALCKSVVELWQSYICLHNFLIGG
                     YTDDDIIDSYQRHFMSCNKYESFTDLCENTVLLSDDVKLTLEENITSDTNLLNYLQQI
                     SDNIFVYYARERVSNSLVWYTKIVSPDFFLHMNNSGAININNKIYHTGDGIKVRESKM
                     VPFP"
     gene            complement(22771..23589)
                     /locus_tag="KLK84_002472"
     CDS             complement(22771..23589)
                     /locus_tag="KLK84_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HBE2009761.1"
                     /translation="MSKHELSLVEVTHYTDPEVLAIVKDFHVRGNFASLPEFAERTFV
                     SAVPLAHLEKFENKEVLFRPGFSSVINISSSHNFSRERLPSGINFCDKNKLSIRTIEK
                     LLVNAFSSPDPGSVRRPYPSGGALYPIEVFLCRLSENTENWQAGTNVYHYLPLSQALE
                     PVATCNTQSLYRSLSGGDSERLGKPHFALVYCIIFEKALFKYRYRGYRMALMETGSMY
                     QNAVLVADQIGLKNRVWAGYTDSYVAKTMNLDQRTVAPLIVQFFGDVNDDKCLQ"
     gene            complement(23591..24445)
                     /locus_tag="KLK84_002473"
     CDS             complement(23591..24445)
                     /locus_tag="KLK84_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McbB family protein"
                     /protein_id="HBE2009762.1"
                     /translation="MINILPFEIISRNTKTLLITYISSVDITHEGMKKVLESLRSKQG
                     IISEYLLDKLLDESLIDKDKGKEFLITTGVINKTKTSPLWVNSVIISDVPHLFSNARE
                     QWKSDGVFVSHIIDIKDNNINVSDSTLIWLHLENYHSDIVKRIYSKFESNPGVAFIQS
                     YYLKESFRIDGVYSPDLGTPCHFCHIERWLSREEKSFRRNEMSWANLLQLLKKYQMTL
                     PALALGESERGFSYHLIKRRLQELTGTSLVKSHVDNFMSSVSADLITCILCKEPVIHW
                     QACSCLER"
BASE COUNT         6443 a         5569 c         6451 g         5987 t
ORIGIN      
        1 aatgagcagg cgactcagga actggcatca acaagtacca ggctatctgg tggaatgtat
       61 gctgatgctc atgctgaatg gggggttaaa cctaaaattc ttggtgttgg tggtggggta
      121 ggcatcagag gtggatttaa ggggagcctg gatgcaacgg atgatgactc acattcagca
      181 agtagtggca gcagggctac ccatgatgct cgccatgata tcgatgctaa agccagtaag
      241 gactttaaag aggccagcga ttactttacc agtcgcaaag tcagtgaatc cggcagccat
      301 actgacaata atgccgattc ccgcgtggac cagttgtctg cagccctgaa ctcagcaaaa
      361 cagagttacg accagtacac gacgaatatg acccgcagcc atgaatatgc ggaaatggcc
      421 tcccgtactg aaagcatgag cgggcagatg agtgaagact tgtcgcaaca gtttgcacag
      481 tatgtgatga aacgcgcacc gcaggatgca gaagctattc tgacaaatac cagttcgccg
      541 gagattgcag aacgccgtcg ggctatggcg tggtcttttg tgcaggaaca ggtacagcct
      601 ggtgttgata atgcctggcg tgaatcccgt ggggatattg gtaaaggaat ggagagcgta
      661 ccttcgggtg gtggcagcca ggatattatt gctgaccatc aggggcatca ggccattatt
      721 gatcaaagaa cgcaggacag taatatccgt aatgatgtaa aacatcaggt tgataatatg
      781 gtcacagaat ataaagggaa tatcggagat actcagaata gtattcatgg cgaagaaaat
      841 attgtagaca gacaatattc agagttaaaa aataatcaca aacaggaaga aattcagcaa
      901 aacaacagat ataatgaaga aaataaacga cagatattaa tgccaacacc gtctgaagat
      961 gcgttaaaac agatggtgga tgataagaaa gaacgattga aggggccatt atgatcactc
     1021 actatgatat aaaaatggag atgcagaaac taaaggaggt actctctgta gagggtgtta
     1081 atatcccatc attattacaa gtcataaagc cgggaactta tgtgttttta tgggttttat
     1141 tatggccaac ttttctgcgt ttagtatcgg ttaaaagtga tgtcagggat gttggctttg
     1201 atatatgtgc tagtggaatg atggggttcc tgctttttgt ggctattaca aatggaatga
     1261 tgttatacct tgcgattcct gatagtttca gaaaggattc aaaaataata aattttatgt
     1321 attctaagag taagacatat attttgttgt ttttgattgt tttttctatg gtttcgttta
     1381 tgcattctat cttatatgtt tttgcactaa tgattacatt catattattt ttcttggtgt
     1441 ataccataga tataaatcgt tataatctct cagctatagc ctcagtaata ggactattca
     1501 aaaaagagtc tgttagttaa aaggatattt tggatgaaaa caaaaaaatt gatgatggtt
     1561 gcactggtca gttccactct ggccctttca gggtgtggtg cgatgagcac agcaatcaag
     1621 aagcgtaacc ttgaggtgaa gactcagatg agtgagacca tctggcttga acccgccagc
     1681 gaacgcacgg tatttctgca gatcaaaaac acgtctgata aagacatgag tgggctgcag
     1741 ggcaaaattg ctgatgctgt gaaagcaaaa ggatatcagg tggtgacttc tccggataaa
     1801 gcctactact ggattcaggc gaatgtgctg aaggccgata agatggatct gcgggagtct
     1861 cagggatggc tgaaccgtgg ttatgaaggc gcagctgttg gtgcagcgtt aggtgccggt
     1921 attaccggat ataactcaaa ttctgccggt gccacactcg gtgtaggcct tgctgctggt
     1981 ctggtgggta tggctgcaga tgcgatggtg gaagatgtga actataccat gatcacggat
     2041 gtacagattg cagagcgtac taaggcaacg gtgacaacgg ataatgttgc cgccctgcgt
     2101 cagggcacat ccggtgcgaa aattcagacc agtactgaaa cgggtaacca gcataaatac
     2161 cagacccgtg tggtctcaaa tgcgaacaag gttaacctga aatttgaaga ggcgaagcct
     2221 gttctcgaag accagctggc caaatcaatc gcaaatattc tctgaacctc gtcaggaggc
     2281 tgatctgacc ggcctccttg ctgacgcctc gcgttgctcg ttgtccaacc ccgggatgta
     2341 acaaaataaa ttttgtcacc tcccggcgtt tccgaaataa atatgaaaac actgagcggt
     2401 tgtgcaaatt ctttgtgcga ccgtgaagtg ttgccggcga agccgggatc ttccggagaa
     2461 agtcattatg acatcagaca atatcgctga atatgttggt gagaataatc aatttactga
     2521 gaagcctgta actgaatacc ccccggtgat acaggataat acagtgacca ctaaagacta
     2581 tctgtacagc aataatattg atactgacct tttagtggtt atcggagggc ttattgttat
     2641 attgtttctc atgatgctta tcatgtactt tattaattct ctcaggcata aggaattaac
     2701 tgaaatactg acaaaaatat cttttcctga aaaatccggg agcaatttgc ttaaaccgga
     2761 ggcggtcagc tgtaatttgt tgtccctgtg tgtgctggca ccggtattct gtacacccag
     2821 aaagctggct gaagtttcca gggagatcat cgtggcgagt gcgcaggaaa ttgcatggac
     2881 gccggggagc agtcgtattg atggccacca ggcggagaat atcacctggg cgatttcatc
     2941 gcagtctcct gccgataagt atgatctcat caataaaacg tcctgtgttt ctcctgacag
     3001 tggccgtttt tatgaaccgg acacagttct ggatcaccgt gaacgggtac gcctttttat
     3061 tcagatccag tttccgcatg ggagtgactg gtctgaagta ttgcgcctga ctgacattgt
     3121 tacagagaag gtctcttccg gcaggactgc aggcgggcat caccaccggc atacagggta
     3181 cacctggtct gtttctgtga attaacccca catcatcctt ttctgccctg ttccagattt
     3241 tcccggagtt tcatcatgag ttttaacgca aaggatatga cccagggcgg tcagattgcg
     3301 tccatgcgta tccgcatgtt cagccagatc gccaatatca tgctttactg cctgtttatt
     3361 tttttctgga tcctcattgg tctggtctta tgggtaaaaa taagctggca gacgtttatc
     3421 aacggctgta tttactggtg gtgtaccacg ctggaaggca tgcgggattt aataaaatcc
     3481 cagccggtct atgagatcca gtattacggc aaaaccttcc ggatgaacgc tgcccaggta
     3541 ctgcatgata aatatatgat ctggtgcgga gagcagctat ggtccgcatt cgttctggcc
     3601 acagttgtgg cactggttat ttgcctgatc accttctttg ttgtctcctg gattctgggg
     3661 catcagggga aacagcagag tgaaaatgag gtgaccggcg ggcgtcagct gacggataac
     3721 ccgaaagaag tggcccggat gctgaaaaaa gacggcaagg actccgatat ccggattggc
     3781 gacctgccga ttatccggga ttccgaaatc cagaacttct gtctgcacgg cacggtcggg
     3841 gccggtaagt cggaggttat ccgtcgtctg gccaactacg cccgtcagcg cggtgatatg
     3901 gtggtgattt atgaccgttc aggggaattt gttaaaagtt actatgatcc ctccatcgat
     3961 aaaatcctga atccgctgga tgcccgctgt gccgcatggg atttgtggaa ggagtgcctg
     4021 acacagccgg attttgataa taccgcgaac acgctgatcc ctatgggtac aaaagaggac
     4081 ccgttctggc agggttcagg acgtaccatt tttgcggaag cggcgtacct gatgcgtaat
     4141 gaccccaacc gcagctacag caaactggtg gacacactgc tttccatcaa aattgaaaaa
     4201 ctgcgtacct tcctgcgtaa ttcaccggca gccaacctgg tggaagagaa aattgagaaa
     4261 acggcgattt ccatccgtgc tgtgctgacc aactacgtga aagccatccg ttacctgcag
     4321 gggattgagc ataacggtga gtcttttacc atccgtgact ggatgcgtgg tgtccgggaa
     4381 gatcagaaaa acggctggct gtttatttcg tcgaacgccg acacccatgc ctccctgaag
     4441 ccggtgatct ccatgtggct gtccattgcc attcgtggtc tgctggcaat gggagaaaac
     4501 cgtaaccgtc gtgtgtggtt tttctgtgac gagttaccca cgttacacaa actgccggac
     4561 ctggtggaga tcctgccgga agcccgtaag ttcggtggct gttatgtgtt tggtatccag
     4621 tcctatgccc agctggaaga tatctacggt gagaaagcgg cggccacgct gtttgacgtc
     4681 atgaacaccc gtgccttttt ccgttccccg agccataaga ttgcagagtt cgctgccggt
     4741 gaaattggtg agaaagagca cctgaaagcc agcgagcagt attcctacgg tgctgacccg
     4801 gtacgtgacg gggtatcgac cggtaaggat atggagcgtc agacgctggt cagttattcc
     4861 gacattcagt ctctgccgga tctgacctgc tatgtcactc tgcccggacc gtatccggca
     4921 gtaaaactct ctctgaaata tcaggcacga ccgaaggtcg ctccggagtt tattccgcgt
     4981 gacatcaacc cggaaatgga gaaccgtctg agtgccgtac ttgctgcaag ggaagcagaa
     5041 ggtcgccaga tggccagcct cttcgaaccg gaggttgcct ccggagaagg cgtgactcag
     5101 gctgaacagc cacaacagcc acaacagcca caacagccac aacagccaca acagccacaa
     5161 cagccacaac agccacaaca gccacaacag ccagtgtctt ctgtcatcaa tgataagaag
     5221 tcagatgcag gtgtgagtgt tccggcaggg gggatcgagc aggagctgaa aatgaaaccg
     5281 gaagaagaga tggaacagca actgccaccc gggatcagtg aatccggtga agtggtggat
     5341 atggccgctt atgaggcatg gcaacaggaa aatcatccgg acatccagca gcagatgcag
     5401 cgtcgtgaag aggtgaacat taatgtgcac cgggagcgtg gggaggatgt tgagccggga
     5461 gatgatttct gatgttgagt ttcagcgttg ttaaatcggc aggcagtgcc ggaaactatt
     5521 acaccgacaa ggataattac tatgtgctgg gcagcatggg agaacgctgg gctggtcagg
     5581 gggctgaaca gcttgggctg cagggcagcg tcgataagga tgtttttacc cgtcttctgg
     5641 agggcaggct gccggacggg gcggatttaa gccgcatgca ggatggcagt aataagcatc
     5701 gtcccggcta cgacctgacc ttctccgccc ccaaaagtgt ctccatgatg gccatgctcg
     5761 ggggggataa acgtcttatt gatgcacata accaggccgt ggattttgct gtccgtcagg
     5821 tggaggcgct ggcttccaca cgggtgatga cggacggaca gtcagaaacg gtgctgaccg
     5881 gtaatctggt gatggcactg tttaaccatg acaccagtcg cgatcaggac ccacagttac
     5941 acacgcatgt agtggtggct aatgtcacgc agcataacgg cgagtggaag acactgagca
     6001 gtgacaaagt ggggaaaacc ggattttccg agaacgtgct agcgaaccgg attgcgttcg
     6061 ggaaaatata tcagagtgag ttacgtcagc gcgttgaggc gctgggttat gaaaccgaag
     6121 tggtcggtaa gcacggtatg tgggagatgc cgggcgtacc ggtggaggcc ttttccagtc
     6181 gttcacaggc tatcagggag gccgtcggtg aagacgcctc gctgaaatcc cgggatgtgg
     6241 cggcactgga tacgcgaaaa tccaaacagc acgtcgatcc ggaaatcaga atggctgaat
     6301 ggatgcagac gctgaaggaa accgggttcg acatccgggc atatcgtgac gcggcggatc
     6361 agcgtgcgga gatccgcacg caggcgcccg ggcctgcttc acaggacggg ccggatgtgc
     6421 agcaggcggt gacacaggcg attgccggat taagtgaacg aaaagtgcag ttcacgtata
     6481 cggacgtgct ggccaggacg gtcggcatac tgccgccgga aaatggtgtg attgaacggg
     6541 cacgcgccgg tatcgatgag gccatcagcc gtgagcagct tatccccctc gaccgtgaga
     6601 aggggctgtt cacatcagga attcatgtgc tcgatgagct gtccgtccgg gcactcagtc
     6661 gtgacatcat gaaacagaac cgggtgaccg tacatccgga gaaaagtgtc ccccggacgg
     6721 ctggttacag cgatgcggtc agtgttctgg cacaggaccg tccgtcgctg gccattgtgt
     6781 ccgggcaggg cggtgcagcc gggcagcgtg agcgggtggc tgaactggtc atgatggccc
     6841 gggagcaggg gcgggaggtg cagattatcg ctgctgaccg tcgctcgcag atgaacctga
     6901 agcaggatga acggctgtcc ggtgagctga taaccggacg tcgtcagctg caggagggca
     6961 tggtcttcac gccgggcagt actgttatcg tcgaccaggg cgaaaaactc tccctgaaag
     7021 agacgttaac cctgctggac ggtgccgcac gtcataacgt acaggtcctg ataaccgaca
     7081 gcgggcagcg aaccggtaca ggcagtgcgc tgatggccat gaaggatgcc ggggtgaaca
     7141 cgtatcgctg gcagggcgga gaacagcgac cggcaaccat catcagtgaa ccggaccgga
     7201 atgtccgcta tgaccggctg gccggagatt ttgcggccag cgtgaaagcc ggagaagaga
     7261 gcgtggcaca ggtcagcggg gtacgggaac aggccatact gacacaggcc attcgcagtg
     7321 agctgaaaac acagggcgtg ctcggacacc cggaggtgac catgaccgcc ctgtcaccgg
     7381 tctggctgga cagccggagc cgttatctgc gggatatgta ccgaccgggg atggtgatgg
     7441 agcagtggaa cccggagaca cgcagtcatg accgctatgt tatcgaccgg gtgacggcgc
     7501 agagtcacag cctgaccctg cgggatgcgc agggcgaaac gcaggtggtg cgtatttcct
     7561 ccctggacag cagctggtcg ctgttccggc cggaaaaaat gccggtggca gacggcgagc
     7621 gactgagggt gacagggaaa attcccggac tccgcgtctc cggcggtgac cgcctgcagg
     7681 tggcatccgt cagtgaagat gcgatgacgg ttgttgtgcc ggggcgggct gaaccggcca
     7741 ccctgcctgt ggctgattca ccgttcacgg cactgaagct ggagaacggc tgggtggaaa
     7801 cgcccgggca ttccgtcagt gacagtgcga cggtgtttgc ctccgtcaca cagatggcaa
     7861 tggacaacgc caccctgaac ggtctggccc gcagcggtcg tgatgtccgg ctgtattcct
     7921 cactggatga aacccgtact gcggaaaaac ttgcccgcca tccctccttt acggtggttt
     7981 ctgagcagat aaaggcgcgg gccggtgaga catcgctgga aaccgctatc agtctgcaga
     8041 aaaccgggct gcacacgccg gcacagcagg ccattcatct ggcccttcct gtgctggaaa
     8101 gtaaaaacct ggccttcagc atggtggacc tgctgacaga ggcgaagtcg tttgctgcag
     8161 aaggaaccgg ttttactgaa ctgggagggg aaatcaatgc gcagataaaa cgcggtgact
     8221 tactgtatgt ggatgtggca aaaggctatg gcacaggcct gctggtttcc cgtgcgtcgt
     8281 atgaggcaga aaaaagtatt cttcgccata ttctcgaagg taaggaggcg gtcacgccgc
     8341 tgatggagag agtacccggc gaactcatgg agacgttaac gtcgggacag cgtgccgcca
     8401 cccgtatgat actggaaacg tccgaccgtt tcacggtggt acagggttat gccggtgtgg
     8461 gtaagaccac acagttccgg gcggtgatgt cagccgtgaa catgctgccg gcgagtgagc
     8521 gtccccgagt tgtggggctg gggcccacgc accgtgcggt cggtgagatg cgcagcgccg
     8581 gcgtggatgc acagacgctg gcgtcctttc tgcatgacac gcagctgcag cagcgcagcg
     8641 gagaaacgcc ggatttcagc aacacgctgt tcctgctcga tgagagctca atggtgggca
     8701 ataccgacat ggcacgggca tacgccctga ttgcggccgg tggcggtcgt gccgtggcca
     8761 gtggtgacac ggaccagctg caggccatcg cgcccggtca gcctttccgt ctccagcaga
     8821 cgcgcagtgc tgccgatgtg gtcatcatga aggagattgt gcgtcagacg ccggaactgc
     8881 gggaggcggt atacagcctg attaaccggg atgtggaaag ggcactgtcc gggcttgaga
     8941 gtgtgaaacc gtctcaggtt ccccgtcagg agggcgcatg ggcaccggag cactccgtga
     9001 cggagttcag tcacagccag gaagcgaaac tggcagaagc gcagcagaag gcgatgctga
     9061 aaggtgagac ttttccggat gtccccatga cactgtatga agccattgtc cgcgactata
     9121 ccggcaggac accggaagca cgggagcaga cgctgattgt cacgcacctg aatgaggacc
     9181 ggcgcgtact gaacagcatg attcatgatg cacgggaaaa ggccggtgag ctggggaaag
     9241 agcaggtcat ggtgcctgtc ctgaacacag cgaatatacg tgacggggag ctgcgtcgtc
     9301 tctccacctg ggagaataac ccggatgccc ttgccctggt ggatagtgtg tatcaccgga
     9361 ttgccggtat cagcaaggat gacgggctga taaccctgga ggatgcggaa ggtaacacgc
     9421 ggctgatttc gccccgggag gcggtggctg aaggtgtcac actgtacacc ccggacaaaa
     9481 tccgggtggg gaccggcgac cggatgcgct tcacgaagag tgaccgggag cgcggttatg
     9541 tggccaacag cgtctggacg gtgacagcgg tttccggtga cagtgtcacg ctgtcagacg
     9601 gacagcagac ccgggtgatt cgccccggcc aggagcgggc agagcaacat attgacctgg
     9661 cttatgccat caccgcccac ggtgcgcagg gggcgagtga aacctttgcc atcgcgcttg
     9721 aaggcacgga aggtaaccgg aaactgatgg ccggctttga gtcagcctac gtggccctgt
     9781 cgcgtatgaa gcagcatgtg caggtgtaca ccgataaccg tcagggctgg acggatgcca
     9841 ttaacaatgc cgtacagaaa ggaactgccc acgatgtgct ggagccgaaa ccggaccggg
     9901 aggtcatgaa tgcgcagcgg ctgttcagta cggcacggga actgcgggac gtggcggcag
     9961 gccgtgccgt tctccgtcag gcagggctgg ccgggggaga cagccctgca cggtttattg
    10021 caccgggacg taaatatccg cagccgtatg tggcactgcc ggcgtttgac cgtaacggca
    10081 agtcagctgg tatctggctg aacccactga ccacggatga cggaaacggg ctgcggggat
    10141 tcagtggtga aggacgggtg aaaggcagcg gggatgcgca gttcgtggcc ctgcagggca
    10201 gccgtaacgg agagagcctg ctggctgata atatgcagga tggcgtccgg attgcccgtg
    10261 ataatcctga cagcggtgtg gtggtgagaa ttgccggtga aggtcgtccg tggaatcccg
    10321 gtgccatcac cggtggccgc gtgtgggggg atatcccgga taacagcgtc cagccggggg
    10381 ccggaaatgg cgaaccggtc acggcagagg tactggcaca gcggcaggct gaagaggcca
    10441 tccgccgtga aacggaacgc cgcgcagatg aaattgtcag gaaaatggca gagaacaaac
    10501 ctgacctgcc ggatggcaaa acagagctgg ctgtcaggga tattgccgga caggagcgtg
    10561 accggtctgc catatctgaa cgggaaaccg cactgccgga gagtgtgctg cgtgaatcac
    10621 aacgggagcg ggaagcggtc cgggaggttg cccgggaaaa tctgctgcag gagcgtctgc
    10681 agcagatgga gcgggatatg gttcgtgacc tgcagaaaga gaaaaccctg ggcggagact
    10741 gatacaggaa gacaaacact gatgacaacg gataacacga acacgacacg taacgattca
    10801 ctggctgcac ggaccgatac ctggttgcag tcgtttctgg tctggtcacc cggacagcgg
    10861 gatatcatca aaacggtggc gctggtgctg atggtactgg accacatcaa tctgatattc
    10921 cagctgaagc aggaatggat gtttctggcc gggcgaggag cctttccgct gtttgccctg
    10981 gtgtgggggc tgaatctgtc ccgtcatgcg catatccggc aaccggccat taaccggctg
    11041 tggggatggg gaattattgc ccagttcgca tattatctgg ccggctttcc ctggtatgag
    11101 gggaatatcc tgtttgcctt tgcagtggcg gcacaggtgc tgacgtggtg tgaaacgcgc
    11161 agtgggtggc gtacagcagc ggccattctt ctgatggccc tgtgggggcc tttgtccggc
    11221 accagttacg gcattgccgg gctgctgatg ctggcggtca gttaccgact gtaccgggcg
    11281 gaagacagag cggaacgtct ggcgctgctt gcatgcctgc tggccgttat tccggcactt
    11341 aaccttgcct ccagtgatgc agcggcggtg gccggtcttg taatgacggt gctgaccgtt
    11401 ggtctggtgt cgtgtgcagg aaaatcatta ccccgtttct ggcccgggga ttttttcccg
    11461 gtattctacg cctgtcatct ggctgtgctg ggcgttctgg cgttgtgact ggtatgatac
    11521 tgacgctgat tctgattatt aataaacaac ataacacagg atatttatga aaattacgga
    11581 tcacaaactc tctgaaggta ttgccctgac atttcgtgtt cctgaaggaa atataaaaca
    11641 tcctttgatt atcttatgcc atgggttttg cggtatcagg aatgttctgc tcccttgctt
    11701 tgcgaatgcc ttcacagaag caggattcgc aacgattact tttgattatc gaggattcgg
    11761 tgagagtgat ggagaacgag ggcgtctggt tcctgcaatg cagaccgaag atattatttc
    11821 agttatcaat tgggcagaaa agcaggaatg tattgataat cagcgtattg ggttatgggg
    11881 aacatcgctg ggaggggggc atgtcttcag tgcagctgcg caggaccaac gcgtaaaatg
    11941 tattgtcagc cagctggctt ttgctgacgg tgatgttctg gttaccggag agatgaatga
    12001 atcggagaga gcgtcttttc tgtccacgct gaataaaatg gcagagaaga agaaaaacac
    12061 cgggaaagag atgtttgtgg gggttacccg ggtactgagt gataatgaat caaaggtatt
    12121 cttcgagaag gtaaaaggtc agtatcctga aatggatata aaaatacctt tccttacggt
    12181 tatggagacg ttgcaatata aaccggcaga gagcgcagcc aaagtacagt gcccagtttt
    12241 gattgttatt gccggccagg atagtgttaa tccaccagaa cagggaaaag ccttatatga
    12301 tgctgttgct tctggtacta aggaattata cgaggaagct gacgcatgcc attatgatat
    12361 ttatgaaggc gcgttttttg aacgtgtggc ggcagtacag acgcaatggt ttaaaaaaca
    12421 tctttaattg tcggtattaa tatgtcagag gtcgttgagg tcagcttaat atcatagtga
    12481 gtagtttcaa cgacctggag gacatatttt cacaaaggtc agggcaatca tgacagagca
    12541 gaaacgaccg gtactgacac tgaaacggaa aacggaaggg gaaacaccga cccggagccg
    12601 gaaaaccatc atcaatgtca ccacgccacc aaaatggaag gtgaaaaagc agaaactggc
    12661 ggagaaggct gcccgggaag cagagctgac agcaaaaaaa gcgcaggcca gacaggcgct
    12721 gtccatttat ctgaacctgc cctcgttgga tgaggccgtg aacaccctga aaccctggtg
    12781 gccgggatta tttgacggtg acacaccccg acttctggcc tgcggtatcc gggacgtgtt
    12841 actggaagac gtggcgcagc ggaatatccc gctctcgcat aaaaaactgc gcagggcgct
    12901 gaaggccatc acccgttcag aaagctatct gtgtgccatg aaagccggtg cctgccggta
    12961 tgacacggaa gggtatgtga cggagcatat ttctcaggag gaagaagtgt atgcggcaga
    13021 gcgtctggat aaaatccgcc gccagaaccg gataaaggca gaacttcagg ccgtgcttga
    13081 tgaacaataa aaaagcctcc ttcacactaa aggaggcgga aaaataaggc ttctctgaaa
    13141 ccttcttcca tacccggcag aagaaggtta taaaaattta taacacattc accggcctgg
    13201 attaaagggg gaaggaaaat gaacggaaca gggcgtgatt cgcaaaactc cggggtatgg
    13261 cgctaccggc aggtcggacg ccgggctgtc cggctggaag tcagggggga ctggccggga
    13321 gccgctgagg catggctacg ggctgcgcag tctgctcccc ggacagactg gcagcagttt
    13381 gcccgaaaaa gggctgaaca ctgccaccgg cgctgccggg gaagggtgta acgaaaaagc
    13441 agccttcatt actccaggga ttcagaaggc tgaataatgc atgagatttg ctttttttaa
    13501 tatccggtcg ggaagtatga ccggacagtt ctgaatagca cagagttttt tccgtattaa
    13561 gtcgtgattt tatattttgt gattaatttc acaaaataag gtgttattca gtgtgtgctg
    13621 cgatattcag gattacctga aatacaggtg ccatttatct gatatggaga ataacatgag
    13681 gaaatatatt cctctggtat tatttatctt ttcatggccg gtattgtgtg cagatattca
    13741 tggacgggtt gttcgtgtac tggacggtga taccatcgag gtcatggact cacggaaggc
    13801 agtgcggatc aggctgataa atattgatgc accggaaaag aaacaggatt atggccggtg
    13861 gtcaacggaa atgatgaaat cactggttgc aggaaaaacg gttaccgtca catattttca
    13921 gcgagatcgt tatggtcgta ttctcgggca ggtttatgca ccagacggaa tgaatgttaa
    13981 tcagtttatg gtccgggccg gggctgcctg ggtatatgag cagtacaaca cagatcccgt
    14041 gctgcctgta cttcagaacg aagccagaca acaaaaacgt gggctctggt cagatgctga
    14101 tccggttccc ccctggatat ggagacaccg taaataaagc gatggcaatc ttatcattca
    14161 tctgttctca gcgggaataa aaatggcaaa aacaaaacag gaatggctgt atcagttacg
    14221 gcgatgttca tcagtcaata cgctggaaaa aatcattcat aaaaacagag acagtttatc
    14281 gaccagtgaa cgtgaatcat ttaattcagc agcagaccac cggttagcgg aattaatcac
    14341 cggaaagtta tatggcagga taccaaaaga gatatggaaa tatgtaaggt aataattcag
    14401 caataattaa ttaccaggag cagataaaca atgaagctga ttatttttat tctcattgtt
    14461 ctgattattg cagcgttact tatccggatt atcctcagaa gtgtaaatca gcattctcct
    14521 ttgctgatgc aattgcatgc ggcaggtata cggaccggtg atgcagagcg aatactgtcc
    14581 ggcggtgaat actggcaacg tcagaagacg ctgctgacag aaagggaagt cagttttatg
    14641 aaaggactgt tcagaattgt ggatatgaag cggtggtatc tgtgtccgca ggtacgggtc
    14701 gcagatatcg tccagctgaa cgggaatatc cggccacgat cacgccagtg gtggcagtta
    14761 ttcaggatgg tgtctcagtg gcatgttgat gtggttatcg ttgagcggcg ttcgttcagt
    14821 attgttgcag cagtagagct ggatgatgcc agccatttac gacctgaacg cagacgccgg
    14881 gatattcttc tggaagaggt tctgaggcag gctggtattc cgttgctcag aagccacgat
    14941 gccagaaaac tgctgcagat gaccggagaa tggctgaata caacaggggc tgcccagcag
    15001 tcaccggaac atcgtagctg acgccttcgc gttgctcagt tgtccaaccc cggaaacggg
    15061 aaaaagcaag ttttccccgc tcccggcgtt tccataactg aaaaccatac tatttcacag
    15121 tttaaatcac attaaacgac agtaatcccc gttgatttgt gcgccaacac agatcttcgt
    15181 cacaattctc aagtcgctga tttcaaaaaa ctgtagtatc ctctgcgaaa cgatccctgt
    15241 ttgagtattg aggaggcgag atgtcgcaga cagaaaatgc agtgacttcc tcatcaggta
    15301 caaagcgagc atacagaaaa ggtaatcctc tgacgcttgc agaacgtcag caggcatccc
    15361 tggcaagaaa acgagctaca cataaagaac tcagagtgtt cattcctgct gcgttaaaag
    15421 cgcaattgca ggtgatgtgt gaagctgagg gcgttaccca ggctgagatg attgccgaac
    15481 tgataaagca gaagagtgct tttagctaac taaaatgtag cgacatcaca ttcttgcctg
    15541 tttctggtgt gcgtgataga ttgctgatcg tttaaggaat tttgtggctg gccacgccgt
    15601 aaggtggcag ggaactggtt ctgatgtgga tttacaggag ccagaaaagt gaaaaccccg
    15661 ataatcttct ctaactttgg cgagtacgaa agattaacgg ggctaacaag aaactgcata
    15721 gaagctgttg ctctatgcgg ggagtatagt tatatgcccg gaaaagttca agacttcttt
    15781 ctgtgctcac tccttctgtg caacataagt gcaggaaggt gtgactgatc ttcaacaaac
    15841 gtattaccgc caggtaaaga acccgaatcc ggtgtttaca ccccgtaaag gtgccggaac
    15901 gctgaagttc tgcgaaaaac tgatggaaaa ggcggtgggc ttcacctccc gttttgattt
    15961 cgccattcat gtggcgcatg cccgttcgcg tggtctgcgc cggcgcatgc caccggtgct
    16021 gcgtcgacgt gccattgatg cgctgctgca ggggctgtgt ttccactatg acccgctggc
    16081 caaccgcgtc cagtgctcca tcaccacgct ggccattgag tgcggactgg cgacagagtc
    16141 cggtgccgga aaactctcca tcacccgtgc cacccgtgcc ctgacgttcc tggctgagct
    16201 ggggttgatt acctaccaga cggaatatga cccgcttatc ggctgctaca ttccgaccga
    16261 catcacgttc acaccggcac tgtttgccgc tctcgatgtg tctgaggatg cggtggcagc
    16321 tgcgcgccgc agtcgtgttg aatgggaaaa cagacagcgc aaaaagcagg ggctggatac
    16381 cctgggtatg gatgagctaa tagcgaaagc ctggcgtttt gtgcgtgagc gtttccgcag
    16441 ttaccagaca gagcttaagt cccgtggaat aaaacgtgcc cgtgcgcgtc gtgatgcgaa
    16501 cagggaacgt caggatatcg tcaccctggt gaaacggcag ctgacgcgtg aaatctcgga
    16561 agggcgcttc actgctaatg gtgaggcggt aaaacgcgaa gtggagcgtc gtgtgaagga
    16621 gcgcatgatt ctgtcacgta accgtaatta cagccggctg gccacagctt ccccctgaaa
    16681 gtgacctcct cagaataatc cggcacgcgc cggaggcttc cgcacgtctg aagcccgcca
    16741 gcgcacaaaa aatcagcacc acatacaaaa aacaatctca tgatccagct tctggtgcat
    16801 ccggtccccc ctgttttcga tacaaaacac gcctcactga cggggaattt tgcttatcca
    16861 cattaaactg caagggactt ccccataagg ttacaaccgt tcatgtcata aagcgccatc
    16921 cgccagcgtt acagggtgca atgtatcttt taaacacctg tttatatctc ctttaaacta
    16981 cttaattaca ttcatttaaa aagaaaacct gttcactgcc tgtcctgtgg acagacagat
    17041 atgtacctcc caccgcaagc ggcgggcccc taccggagcc actttagtta caacactcag
    17101 acacaaccac cagaaaaacc ccggtccagc gcagaactga aaccacaaag cccctccctc
    17161 ataactgaaa agcgccgccg tcccgcccaa agggcgggaa caacatcgct tttaattatg
    17221 aatgttgtaa ctatactccg tcatcgctgt cagtcttctc gctggaagta ttgagtacac
    17281 gctcgtaagc ggccctgacg gcccgctaac gcggagatac gccccgactt cgggtaaacc
    17341 ctcgtcggga ccactccgac cgcgcacaga agctctctca tggctgaaaa cgggtatggt
    17401 ctggcagggc aagggatggg taaggtgaaa tctatcaatc agtaccggct gacgccgggc
    17461 ttcggcggtt ttactccggt atcacatgta accacagcgt gccgccttcc atgccgctgg
    17521 cgcggcataa gcatatgttg gtggctgtgt ctgaaaggtt atatactcta cataaaacct
    17581 gacaacacgc agattgccag gcaaatgagc attatgcatc cggttatcgc ctgcctgtga
    17641 aacaatatca ccggggcgaa tatcgatact ctgactcgtt atacccatgc gtgtggcatc
    17701 aaaaaacctg caattgccct ttattgaatg aaagggggag tcaattatga atagcaccac
    17761 acattacgaa aacgccaatt tcctgcgcga gctggccgaa agcctgcctc gtatttttcc
    17821 agaaggcagt acagacaagt ctgcactatt gcaacgcctc gctaatgaag aactggcacg
    17881 ggcggaatac gacgaacaga tccgagcgaa agttgccgct gcccgcgccg ataagcggcc
    17941 gggaatgagt tccgcgcaac tgcgccagca actacaggga cgttatcagg aactgcgcaa
    18001 tgagttatga aatagaatgg cgaccaggtg cgattgagga tataacggcg ttgtttgaat
    18061 atctggtcga gaatgcatct ctttgggatg caaggaacgt gacagagcgt ctgatcactg
    18121 caaccgatcg cctggctgat taccccaggc tgtatgaaac ggatgaacgc tatggtgaag
    18181 gggttcgccg tattagctta cttggacagc atgtacttta tgaagtgaat gatgaggcca
    18241 gtcgtgtcaa tatcctggcg gttgtaggcc agcgtcagaa tccccgattc attctttgag
    18301 tgtccggctt ggaactccat cttcagagaa tcgatcaacg ttctttagct ccttccacta
    18361 acagccgacc tatctcaaac accgcagctg accgtcccga cttcaccgct tcggctttct
    18421 gtccctcaac aaaccagtta ttaccgtccg ttcggaccaa ctcaaacatc catgacatat
    18481 cgtaaacgtg ttccgggatc agcgaccctt ctccggccag aatacccgcc gttatggagt
    18541 ggttgccccc gtgcacaaag ccaatacgcc agggcaacca caggtcaaca tagtggttgt
    18601 ttttatcttg cttccacggc ttccccttat gggttccaat gcgtgaaacg ttatcgatat
    18661 agcgtggtag gctccaaggc cacggcagca ctatgtcgct tgccagcgac aacggataat
    18721 cagcgccgtt catctcacac tctttcatct tttcgtaaga ttgtatttgg gtaagtccct
    18781 taaagaagaa ctcctgcggg gtcatatccg ggcgggcatc tgttccctcc tcagctaccg
    18841 ccagcaagaa atcactctgt actggccgca cgatagcgcg aatgagatct ggtagcgcct
    18901 taacgggtag tttctctgcc acagaaagca gatgattgaa gcgttttttt tcattggcta
    18961 tagttgcttt atctttcttg tttcggttcc agaacatcac tgtctgtagc cctccttatc
    19021 gtgatcagga tgatggtttt cattctaatg gtgtagtagt ttcacgtcac gcgcacgtgc
    19081 atattcttcg aaagcttcgg cgttagccgg tcaaccaggg ggttgaggcc caaccgtacg
    19141 agatcgtacg ctaagttcat tttttgagca gaagaggtga gtaaagaaaa cgagggtttt
    19201 gtttactctt cccgtcaatg ccagtagcca tgcggtttca gggactttca gtgttaataa
    19261 actcggtaat caaatcaacg taaacctgcc taacaacgcc acgactttat tgacttaaaa
    19321 tcatatagtt agcttcttag cgtacgtttc cgctccattg ggcttcaaac cccacaccca
    19381 atgacatgag aaaagctgga tctgattctc aaaatgctgg gatacccatc gagatggaga
    19441 actcagtcaa tgttccattc tttctgaaat atgtgtgggg acactgatct gtcgggagcc
    19501 ggatgcggta gttccgcagg tccggttctg aggaggggcc cgtccgggca accggcgggt
    19561 ctactcacct catcccccta caaccactcc cattcgttta agtattttta aacctgcttc
    19621 ctgtggtatc tttatagata agatcctgtt tatctcagga tttatatcga gaccttctgt
    19681 cattgaaaaa tcacagtgag acaaatcaga atgtctgaac tctgtattat taaactccga
    19741 accagtaaaa tcagatttac gaaggtctgt ttcctcaaat aaacaatcac ggaagcgact
    19801 ggataaaaaa atagatttct gtaaccgtaa actaataaaa tcaacaaatc tcaaatcaca
    19861 atttacaaga gaaaaagtac agggaaacat aatgtctgct gcatttactc cttgtaataa
    19921 acaatctttg aattcacagt caataagttt aaaaaaatta agttttaaat tctttattga
    19981 gcagtttacg aagcgacatt tttcgaactc acaatcgcga aagttaaagt tgtcaagctg
    20041 tattcgttca aatatacaat tgtaataatt tacacctgat aattcacttt cactgaggtg
    20101 tcgttttgtg attctttttt ctattatatc catgtgtcag tctcctgttc ccataacttc
    20161 tttaataaat gccttttctg gagttgtttc attattggat tgaataaaat cactcatatc
    20221 agaataacga tttatactat tttttgaaag catatagaag gggattttat ctcttgttaa
    20281 ttcatcaaaa atatgagttg agaaaaatac agttttcccg tcagcggtga tcttatttat
    20341 cagttcccat aacatcattc tgtactgaat atcaatgcct acagttggtt catccagtaa
    20401 aaataaacgg gcatttttgc aaagcgttag catcaaagat atgataagcc aacgcttctc
    20461 tccataactt accgtgtaag tccttttacg tcttatttta tcatagcgaa tgaaaaaatt
    20521 atcattccat ttatgattaa gctctgtcca tgcctgtttt ttagttaact tattgaagca
    20581 gcaaatcaaa tcaagaacct cctcattcct tagtgtacct gaaagattta tgacttgagg
    20641 tagacttact aattctccac cagagaaatt tcgcttgagg gtcccttcat aattactgac
    20701 tccgcccctg ataaggttaa aaagagtggt cttcccggct ccattttctc caagcagacc
    20761 tatcaactct ccctgctcaa tcttaagcga cagattattg aaaacagggg gtaaatttac
    20821 tttatatgaa aagctcagag agttgatttc taaaagaggt atggtcattg agaactccag
    20881 actggctcag ttttgaaggt tagtgctgaa ataatactta tgattaacat tatactatag
    20941 aaaaatatcg tcatcagaga caactcctga tctgactgga gtattgtaga atagatggcg
    21001 ataggattaa cctgatttat ccagtaaacg atcccctcat tatattttaa tgatacaatt
    21061 cctgatacca tacagacagt tattaataca gaatagatag tgctggctgt ttggaatgtg
    21121 actctgaacg atgccatgaa agtgagtgaa accatcataa atgcatttat gtatatcgat
    21181 tttaaaataa tcattactat ttgataatct ggacttgcct ggaatcctgt tagtacgact
    21241 aaaatgaaaa aaaacacata caaaatagac atgataagtg aggctattaa ttgagaacga
    21301 ataaataata gtcgaccatc catattatgc acgaaggtag caataaaacc actttctctt
    21361 cttccgacca gatacagaca gtaattaaac agaacaacag aaaaagaaat gtagcctaca
    21421 aaccatgata tttgttttcc gagccatgct gtatcaccat aatgaagttc aatttcattg
    21481 aaatgaagga aaaagaacaa cactgtaggt gacataattg tccatattaa gctgaaaggg
    21541 gttctgattt gttccatcag aaaaattatc gccattttta atgtaaccat ttgttatcct
    21601 tatgggaatg gtaccatctt tgattctctg actttaatac cgtccccggt atggtaaatt
    21661 ttattattaa tgtttattgc acctgagtta ttcatatgaa ggaaaaaatc agggcttact
    21721 atttttgtgt accagacaag gctgttactt actctttccc tagcatagta aacaaaaata
    21781 ttatcagaaa tttgttgaag atagtttaat aaatttgtgt ctgacgtaat attttcctca
    21841 agcgttaact tgacatcatc agacagtagt accgtatttt cacacaaatc cgtaaacgac
    21901 tcgtacttgt tgcatgacat aaagtgacgc tggtaactat caataatgtc atcatcagta
    21961 taaccgccaa taagaaagtt gtgcaggcat atatacgatt gccacaattc cactacggat
    22021 ttacaaagtg cttttttcag actattagca taggataatc cggtactgta ttttattcga
    22081 ctgattttgt tttttgtgcc atacagggtt agtactgcgt gtccaggagc tccgggcagg
    22141 gtgatatcga aaatcctgat atctccttct gacctgagag ccagtaaaat ctcatctata
    22201 tgatttatgc ctgttactat ttcactggat atttcagtat tggcttttcc ctgtaaccag
    22261 taaagaagga gggactgtct ctccataaat tcacacaagg aaccttctat ggcatcgttc
    22321 aaactaccat gaaagctaca tccgcatgta tctctgtcag gataaaattt cagatcatct
    22381 gcagcggtta tattgtcgag tgcaattatg actgcaggta tttcttgttg ttcaaggcta
    22441 aacagattaa aggctctgac cgttttaaat ttatgggtta tgatttcatc gcgggtcagt
    22501 gatgagatct gaaaaaatgc ttcggtaaaa gcctttgcag cagatggagg tatcatctca
    22561 tataatgttt tctgaccacc ggtgactatc tcattaaaaa aatgacggcg ttcaaaatat
    22621 tcacctattg ccccctgaat tgacttcaga gtttcaccgg ccgcgctggc tggtgtaata
    22681 cgctcatagt cccatatctg agaaaacgtt ggcatatttc tgttcagttt tggactcatg
    22741 gccattgtgg ccggccatgc ggacataagg ttactgtaga catttatcat cgtttacatc
    22801 tccaaaaaac tgaacgatca gtggcgctac agtcctctga tccagattca ttgtttttgc
    22861 tacgtatgaa tcggtatatc ccgcccatac ccggtttttc agtcctattt gatctgcaac
    22921 caatactgcg ttctgataca tcgaacctgt ttccattaag gccatccggt atcctctgta
    22981 gcgatatttg aacaaagctt tttcaaaaat aatgcaatag acgagagcaa aatggggttt
    23041 accaagacgt tccgaatccc caccggacag gcttcggtag agtgactgag tattacatgt
    23101 agcaacaggt tccagtgcct gacttagcgg caggtagtga taaacattag ttcctgcctg
    23161 ccagttttct gtattttcag ataatctgca taaaaaaact tcaatcgggt acaatgcccc
    23221 cccagaagga taaggtcttc ttacagagcc aggatcaggt gagctgaatg cattgactaa
    23281 taacttttca atagtacgaa tggaaagttt atttttgtcg caaaagttta ttcctgatgg
    23341 gagcctttca cgactaaaat tatgtgatga ggatatgttt attacggagc tgaaacctgg
    23401 cctgaagaga acttctttat tttcaaattt ctccagatgg gcaagaggta ccgcggacac
    23461 gaaagttcgt tcagcaaatt cggggaggga agcaaagtta cctctgacat gaaaatcttt
    23521 aacaatggcc agaacttcag gatctgtgta atgcgttact tccactaaag agagttcgtg
    23581 ttttgacatg ttatctctcc agacagctgc aagcctgcca gtgaattacc ggctctttac
    23641 ataaaataca ggtgattaaa tcagcgctaa cagatgacat aaagttatcg acatgacttt
    23701 taaccagtga agtacctgtc aactcctgaa gtcgccgttt tattaaatga tagctgaatc
    23761 ctctttctga ttcgcctaaa gccaatgctg gtagtgtcat ttggtatttt ttcagtaact
    23821 gaagtaagtt tgcccaggac atttcgttac gtctgaagct tttttcttcc ctgctcagcc
    23881 atctctctat atgacaaaaa tggcagggag taccaagatc aggtgaatat actccatcta
    23941 tcctgaatga ctcctttagg tagtagcttt ggatgaaggc aacaccaggg ttactttcaa
    24001 attttgaata gattcttttt acaatgtctg aatgatagtt ttcgagatgt aaccagatta
    24061 gtgttgaatc gctcacatta atattattat cttttatatc aatgatatga gaaacaaaaa
    24121 caccatcaga tttccattgt tcccgggcat tactgaaaag atgtggaaca tcgcttatta
    24181 tgactgagtt tacccagaga ggtgatgttt tcgttttatt tatcacgcct gtggtaataa
    24241 gaaactcttt gcccttgtct ttatcaataa gcgattcgtc cagtagttta tcaaggaggt
    24301 attcagaaat aataccctgt ttggatcgta ggctttcgag tacttttttc attccctcat
    24361 gtgtaatgtc tactgaagaa atgtaggtaa taagcagggt ctttgtattt ctggaaatta
    24421 tctcaaacgg cagaatattg atcatatctt
//
LOCUS       DAEEUE010000058        24183 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.058, whole genome shotgun sequence.
ACCESSION   DAEEUE010000058 DAEEUE010000000
VERSION     DAEEUE010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24183
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.058"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(25..597)
                     /locus_tag="KLK84_002474"
     CDS             complement(25..597)
                     /locus_tag="KLK84_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HBE2009763.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            815..1675
                     /gene="tesB"
                     /locus_tag="KLK84_002475"
     CDS             815..1675
                     /gene="tesB"
                     /locus_tag="KLK84_002475"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HBE2009764.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRSGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="KLK84_002476"
     CDS             complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="KLK84_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HBE2009765.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="KLK84_002477"
     CDS             complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="KLK84_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBE2009766.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3559..5340)
                     /locus_tag="KLK84_002478"
     CDS             complement(3559..5340)
                     /locus_tag="KLK84_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBE2009767.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5333..7105)
                     /locus_tag="KLK84_002479"
     CDS             complement(5333..7105)
                     /locus_tag="KLK84_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBE2009768.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7135..7593)
                     /gene="decR"
                     /locus_tag="KLK84_002480"
     CDS             complement(7135..7593)
                     /gene="decR"
                     /locus_tag="KLK84_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HBE2009769.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7747..8565)
                     /gene="cof"
                     /locus_tag="KLK84_002481"
     CDS             complement(7747..8565)
                     /gene="cof"
                     /locus_tag="KLK84_002481"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HBE2009770.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRECDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8664..10356
                     /locus_tag="KLK84_002482"
                     /pseudo
     CDS             8664..10356
                     /locus_tag="KLK84_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10421..11116
                     /gene="queC"
                     /locus_tag="KLK84_002483"
     CDS             10421..11116
                     /gene="queC"
                     /locus_tag="KLK84_002483"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HBE2009771.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11168..11566)
                     /gene="fadM"
                     /locus_tag="KLK84_002484"
     CDS             complement(11168..11566)
                     /gene="fadM"
                     /locus_tag="KLK84_002484"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HBE2009772.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11660..12031)
                     /locus_tag="KLK84_002485"
     CDS             complement(11660..12031)
                     /locus_tag="KLK84_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBE2009773.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12182..14053)
                     /gene="ppiD"
                     /locus_tag="KLK84_002486"
     CDS             complement(12182..14053)
                     /gene="ppiD"
                     /locus_tag="KLK84_002486"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBE2009774.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14245..14517)
                     /gene="hupB"
                     /locus_tag="KLK84_002487"
     CDS             complement(14245..14517)
                     /gene="hupB"
                     /locus_tag="KLK84_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HBE2009775.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14726..17080)
                     /gene="lon"
                     /locus_tag="KLK84_002488"
     CDS             complement(14726..17080)
                     /gene="lon"
                     /locus_tag="KLK84_002488"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBE2009776.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17268..18542)
                     /gene="clpX"
                     /locus_tag="KLK84_002489"
     CDS             complement(17268..18542)
                     /gene="clpX"
                     /locus_tag="KLK84_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HBE2009777.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18668..19291)
                     /gene="clpP"
                     /locus_tag="KLK84_002490"
     CDS             complement(18668..19291)
                     /gene="clpP"
                     /locus_tag="KLK84_002490"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBE2009778.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19538..20836)
                     /gene="tig"
                     /locus_tag="KLK84_002491"
     CDS             complement(19538..20836)
                     /gene="tig"
                     /locus_tag="KLK84_002491"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBE2009779.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21180..21497)
                     /gene="bolA"
                     /locus_tag="KLK84_002492"
     CDS             complement(21180..21497)
                     /gene="bolA"
                     /locus_tag="KLK84_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HBE2009780.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21802..22380
                     /locus_tag="KLK84_002493"
     CDS             21802..22380
                     /locus_tag="KLK84_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBE2009781.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22424..23899
                     /gene="ampG"
                     /locus_tag="KLK84_002494"
     CDS             22424..23899
                     /gene="ampG"
                     /locus_tag="KLK84_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HBE2009782.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVMLGGLLDYLALRKTHLT"
BASE COUNT         5665 a         6665 c         5828 g         6025 t
ORIGIN      
        1 ccgggcggag agggttaacg tcgcttaata ttgcgtagga gcgggaactg ccgaagacgg
       61 attcacctga gttggtgaag tcgaaggtac ggtagtcgtt gcgccaccgc tggcctgaac
      121 cggcacggcg gtttgctgta ccggcaccaa tgtcaggtcg gctttagttc cgccctggtt
      181 gatcaccggc tgaacggtat cggtgataaa taccagtttg tcattcacgg taatcgccgc
      241 actcaacaga atacgcgcgt tcggctgaac atctgccggg ttaaatggca gaacaaagct
      301 gaatggtgac tgtttacctt cagtacgcac cgctttctgc gccagcactt ttgacggtgc
      361 atcggctaac gacgcgtcag aaagtgtcac ggtcagcaca gcatcaggcg gcagtgcgac
      421 tttctgacgg atccagacgg taccggagac attcggttgc tggatagcag gttgttgtgt
      481 tgctgaaata gacgtatttg cagccggggc tggcgtctga atatccgcgc ttttatctgc
      541 gcaagccgcc aacgcaatcg caaccgctaa accactggcc atgtgcacga gtttcattca
      601 tttctcctta ttatcaatgc accagcgggc taactttcct cgccggaaga gtggttaaca
      661 aaatagtaac gtcaacaagt gtggcacaca tcacgcattt ctgcctgtaa ttagcccgta
      721 attcagacta tttcacccat cggaccagtg gcaaatttgc gttatactca actcactttg
      781 gcttgctgcg gcagctttgt tactggagag ttatatgagt caggcgctaa aaaatttact
      841 gacattgtta aatctggaaa aaattgagga aggactcttt cgcggccaga gtgaagattt
      901 aggtttacgc caggtgtttg gcggccaggt cgtgggtcag gccttgtatg ctgcaaaaga
      961 gaccgtcccg gaagagcggc tggtacattc gtttcacagc tactttcttc gctcaggcga
     1021 tagtaagaaa ccgattattt atgatgtcga aacgctgcgt gacggtaaca gcttcagcgc
     1081 ccgccgggtt gctgctattc aaaacggcaa accgattttt tatatgacgg cctctttcca
     1141 ggcaccagaa gcgggtttcg aacatcaaaa aacaatgccg tccgcgccag cgcctgatgg
     1201 cctgccttcg gaaacgcaaa tcgcccaatc gctggcgcac ctgctgccgc cagtgcttaa
     1261 agataaattc atctgcgatc gtccgctgga agtccgtccg gtggagtttc ataacccact
     1321 gaaaggtcac gtcgcagaac cacatcgtca ggtgtggatc cgcgcaaatg gtagcgtgcc
     1381 ggatgacctg cgcgttcatc agtatctgct cggttacgct tctgatctta acttcctgcc
     1441 ggtagctcta cagccgcact gcatcggttt tctcgaaccg gggattcaga ttgccaccat
     1501 tgaccattcc atgtggttcc atcgcccgtt taatttgaat gaatggctgc tgtatagcgt
     1561 ggagagcacc tcggcgtcca gcgcacgcgg ctttgtgcgc ggtgagtttt atacccaaga
     1621 cggcgtgctg gttgcctcga ccgttcagga aggggtaatg cgtaatcaca attaaaaaaa
     1681 tagccggagg tgaaaaccgt ccggctattt tttgcagtgc ttgttacgcg ttataggcat
     1741 tctcgccgtg gctgttgacg tccagccctt ctcgctcttg ttcttccggc acacgtaggc
     1801 caaccgtcag atccgccagt ttgtagccaa taaatgccac cacaccggac cagacgatcg
     1861 taatggcgat gctttccaac tgtaccagca actgatggcc catcgtcaca ccttcagcga
     1921 agcccacgcc gcccagagaa ctggcggcaa aaatcccggt catgatacag ccgacaatgc
     1981 cacaaacgcc gtgcacaccg aagacatcgc agggatcatc cacccgcagc aagcgtttga
     2041 gcatggtaac gccccacaaa cccgccagac cagctaccac gccgataatc aacgcgccgc
     2101 caaccccaat gtagccgcac gctggcgtca cgccgaccag accggcaatc gcgccagaac
     2161 acgctcccag cagagaaggc ttaccacgca gcgcccattc accgaagatc cagccaagaa
     2221 ttgccgccgc cgttgcgacc acagtattca caaatgccag tgccgcgatt tcattcgccg
     2281 tgcccactga cccggcgtta aagccaaacc aaccgatata gagaatggca gtcccggtga
     2341 agaccatcgg caggttgtgc ggtttaaacg cctctttacc gaagcctacg cgtttgccta
     2401 tcagatacgc gcccaccaga ccggcgattg ctgcgttaat gtgcaccacc gtgccacccg
     2461 cgaaatccag cgcaccgtga gaagccagca aaccaccgcc ccacaccata tgcgcaatcg
     2521 gaatgtaaga gagcgtcagc cataccacca cgaaaatcaa cacagctgag aagcggattc
     2581 gttccgccag cgccccaact atcaagccta cggtaatgca ggcaaacgat ccctgaaacg
     2641 ctacgtggat atactgataa atgctgccca tcaccgccgt cagttcgatg ttttttagca
     2701 tcaaccagtt aatgttgccg aagaagttgt tgccctcacc aaacgccagc gaataaccgt
     2761 aaaccaccca gagaatacag accagtgcaa acgtcaccgt cacctgcgtc agcatcgaca
     2821 gcacgttttt gccgcgaatc aacccaccgt aaaacagggc aatccccgga atagtcataa
     2881 acagcaccag cgcagtacaa atcatcataa acgcattgtc ggctttatcg gccaccgcag
     2941 gtgcagccat taccagtccc ggcagcatcg ccagtgaagc aagcccagtt tttatcgtcg
     3001 ctatcttcat ttttcgttcc tgttgctgtg tgccagagat tacagcgccg cttcgtcggc
     3061 ttcgccggta cgaatacgaa tgacgcgttg caattcagcg acgaagattt tgccgtcgcc
     3121 aatttttccg gtgtaagccg ccttactgac gatatcgatc acttcatcga gttggtcatc
     3181 agcaatcgcc acatcaattt ttacttttgg caggaagttg acgctgtatt ccgccccccg
     3241 gtacagctcg gcatgccctt tctgacgccc gaaacctttc acttcggtga cggtcaggcc
     3301 ctgaatacca atggaagata acgcttcacg aacgtcttcc agcttgaatg gtttgattat
     3361 cacggtcacc agcttcatag atcccctccg gtcagaattc ggtaatggtt ctgctacacg
     3421 aagaaggtat tgcaagcggt gtgccagaaa tgaaaaacga gaaagagagc aggaagttaa
     3481 gaagattgaa aatgaaaacg cactatgaca gtgcacagtg cgttacattt ttgcaccaac
     3541 gatgaaatat tgcgctattc aggcgctcaa tgattcctct tcacgcacgc tggctgccag
     3601 ctcttcgccc gcaagttgca gttgatacat ctgccagtag cgtccctggg ccgccagcag
     3661 ttgctggtga gtgccctgct ccacggcttg cccacgatga agcaccagaa tggtgtcggc
     3721 atcaataata gttgataagc ggtgagcaat caccaccagc gtggtatgtt cacgcaccgc
     3781 cgccagagca tgttgaatcg cctgttcagt accggagtca atgctggcgg ttgcctcatc
     3841 aaggatcagg atttgcggcg tctccaccag cacgcgcgcc agtgccagca gttgcttttg
     3901 cccgactgag agattatttc cctgctcgcc cagcggcgta taaatacaat cactcatgct
     3961 gcgcgccagc tccgccagtt gcacggtttc cagcgcctgc cagacgcgtt cttcggagat
     4021 atcccgcccc agcgtcacat tagcgaggaa ggtatccgcc agcaccaccg gatcttgctg
     4081 caccattgcc acgccctggc gcagcgcgct atgacttagc gaacttaatg gacgaccatc
     4141 aaggtgaatc tcaccttccg ttagcgggta atagcccatc aataaactgg cgagggtact
     4201 tttaccactg ccggtatgcc cgaccagcgc cacaaagtta cgcgaaggca cagagagatt
     4261 aatgttcttt agcaccagat tgtcatcgcg ataagcaaat gacacgttat cgacttcgat
     4321 ggtgccactc tgtaacgggt aatcatcatt gccatattgc tggcgcggtc cgtccatcag
     4381 ttcaaacacg cgctcaccag caacaacagc ctgttgcagc atcgcctgtt gcgtggtcag
     4441 ttcgattaag ggttcgttaa gtcgcccaag atagctgata aacgcataca acacgcccac
     4501 ttcaatggtg ccgctggcgg agaagccaaa cagcatcaat aagccacaaa gaatgagcga
     4561 tgaaaacaga ctcagcagcg gacgcagcag aaaaccgtcg aggcgcaggg tttgcatcct
     4621 agccatatag tgtgaacgac tggcctcccc catacgttcg ccaaatcgcg cctgctgacg
     4681 aaactgctgg ataacgctca tgccattgat gatttcgtta aagccgtcgt tgatatccgc
     4741 cagataggcg cgcacacggc ggacaatcgg cgtgctgtaa cgctggtata tcaccattac
     4801 caccagcacc accgggaaaa tcattatcgc caccagcgcc attcgccagt cgaggctgaa
     4861 catcgccacc agcatcgcgc ccaccagtgc ggcactgcgc aggacagttg ccactacggt
     4921 aacgtagaga tcgcggatca cttcagtgtc attagtgacg cgggaaatca cctgcccgac
     4981 gggttgggta tcaaactcgc ttaatggctg gcgtaacgca gcatccatca cgtcggtacg
     5041 caactgttgc actgcgccta ctgccgcccg attaaacagc agcgactgcg cgtaatgtag
     5101 cccggcggca aacagttgta gcccaacata cgccgcagcc agccctgcaa ccactttcaa
     5161 cggcaggtta tttttcgcta ccatattgtc gataaaatag ctgataagca gcggcccact
     5221 gacttctgcc gccgccgcaa cccacatcat caggaccgca atccccagcg gtttacgcca
     5281 cggcgaacca tacgctaaca ggcgcttgag agtcggccac agttggctaa aactacgcat
     5341 cgacggcctc ctcgcgaatt tccggaacgt cgtcgagcgc cgcctccagt tgttgatagc
     5401 gatacatatc gcgataccaa ccgctttgtt gcgccagctc atcatgattg ccacgctggg
     5461 cgatatgtcc gtgctgcatc acaataattt cactggcttc cgtcagtgcc gaaaggcgat
     5521 gggcactgat gattaccgtt cttccctgtc cccactgacg caggttatgc aggatctggt
     5581 gctctgtgcg tccgtccacc gccgaaagcg catcatcaag gatgaggatt tccgcgttga
     5641 ctaataacgc tcgagcaatg gagatacgct gtttttgccc accggaaagc atcacgccgc
     5701 gctcgcccac ttctgtatcg taaccttgcg gtagacgcaa aatatcgtca tgtacgctgg
     5761 ctaaccgcgc gacatgctca atctcttgct gggtggcatt cgggcaaccc agcgcgatgt
     5821 tattcgccac ggtgtcagaa aaaaggaatg gcgtctggct gaccaccgcc aggcggctac
     5881 gccagctatc gagttgtaac ttcgtcagag gaatatcatg aaagcgaata tccccctcgc
     5941 tgacgtcgaa atgacgctga atgagcgaca acagggtact tttgccggaa ccagtcggcc
     6001 cgcaaatacc cagcatctga ccgggtttca gggcgaaatt gacgttttcc agcgcaggat
     6061 ggtcagtctg cggatacgtg aactggtgaa tatttacatc cagttcgcca cgcccttccg
     6121 gcacgggttc gctaccatcg ttcaccaccg gcgcttccgc cagcatcgcg cgaatacggc
     6181 tgtatgcagc actaccacgt tccacaatgt taaacatcca tgccagcgcc agcattggcc
     6241 aaatcatcag acctaaatac atcataaaac tggtgagctg gcccagcgtt aaactgccct
     6301 gcaccaccat ccaactaccg ccgccaatcg ccagcaagtt cgccatacca atcgcgatat
     6361 agatggtcgg gtcgaagcga gcatcaatac gcgccacccg catgtttttt ttgccggtat
     6421 cttcggcatc cgcggcaaat aacgccgact ggcgatcttc cagaccaaag gctttgatca
     6481 tgcggatact ggtgaggctt tcctgggtgc ggtcattaag actggaaaac gccgcctgtg
     6541 ccagcttaaa gcgttcatgc aaggcatcgc cgttgcgctt aatcatgatc gccatcactg
     6601 gcatcggtaa cagggcaaat aaggtcaact gccagctaat ttgcgtagac atcataatca
     6661 acacagcgca gcccattacc agtgaatcca ccagcgtcag caccccttct ccggcggcaa
     6721 acacgacgcg atcgacgtca ttggtcgcac gagccatgag atcacccgtg cgatgacgca
     6781 ggtaaaactc cgggtgctgc cggcttagct gacggtaata atcttcacgc agttcaacag
     6841 ccagttggta agacgcgcca aacagcaata cgcgccagac ataacgcagg agataaacca
     6901 caacggcaat cagcaccatg gtggcgatcc acatcaggct cagcccggta gtaaagtgtt
     6961 gttctgtcac gccatcgaca acaataccaa ccacttttgg cggaaccagt tgcagcatcg
     7021 cgataatgac aagcaaggcg acagccccaa gatagcgacg ccattcccga cggaaatacc
     7081 agcttaattg agcaaataat cgcacgcgtt gtgtcctgac ctgattctgg atatttattc
     7141 gatgggtaaa gaagtggtgt atttaatctg ttccatcgcg aagctggaag tgacgtccga
     7201 cagccccggc acgctgttta ccagacgctt ataaaactcg tcgtagcgtt tcatgtcggc
     7261 aacctggacg cgcatcagat aatcgtattc accagccatg cgccagaccc ccagcacttc
     7321 tggcatttcg gtaaccaccg tgacaaagcg gcaataccat tcgctgctgt gatgttgcgt
     7381 ttttatcagc acaaaggcgg tcaggccgag gcctattttt tccggatcca gcagggcgac
     7441 tttgccgata aggataccgt cgtcctccag ccgtttcagg cgcttccagc agggggtggt
     7501 tgtcagatta acggcttcag ccagtgcctg caaagagagg gtgcaatcct gctgcagtaa
     7561 ggccagcagc ttacggtcaa ttttatctaa catagccctt ccacggagaa tttttttctc
     7621 ttttaattct attttaaagg tgaaatcgcg acaatttatt ctgtgcggtt taaacaccac
     7681 ggcacaaaat gacaaattca acgcaaaaaa aaaccgggca attgcccggt gctggaaggg
     7741 atctcgttat tcgggggaat aaggtagatg tggatagtcc agccagtgcg tcaaatagtg
     7801 agagacagcc tgatttcggc aatgtccaat caccggtaaa tgcgggagct ccgcgcgcag
     7861 ttgcggcatc gcattgccca taataaaccc gctaccgacg ctgcctaaca tttcgcgatc
     7921 gttcatcgca tcaccaaagg ccatgcaatc gcgcaacgat aaacctaaat gttgggtccg
     7981 caccgtcaat gcagcgcctt tattgcagcc caccggcagc acttcgaggc aatccgtggc
     8041 ggaaaaacac aaatgtgcac gctcgcctaa tgcttcgtat agctggatct gcaagcgtgt
     8101 aagatcgtcg tgatcgccac agaagcagat cttggtgacg ctgccgagtg gcattttttt
     8161 gacatcgatt atctgataac gaaaaccgct atagacaaat acctgcaaca acgcagggct
     8221 ctctttcccg gtaaaccaac cgtcgtcatt gaagatatgc atgctggctc gggtatccca
     8281 ttgctgatac agcaccagct ccgcgacatc cgcaggtaaa tcatcacgat gtaaaagttc
     8341 accttccaga gaatgcacgc gcgttccgtt gccggtaatc aaatatgcat ccagcgatag
     8401 cgccccgaga atatgctgca tctccagcgc atgacgcccc gtggcaaaag tgagggtaat
     8461 gtcgcattca cgcagtcgcg ccaaagtaga gagggttttc tcacctaaat gatggtcagg
     8521 catcaataaa gtgccatcca tatcaaatgc taccagacga gccatttctt tctccactct
     8581 gtgacacggt taatggtgat tgagtatcac ctgatatata cggaagtaat agtgaatagt
     8641 taataatatt gttccgggtt tatatgcgat tgctcaaccg tcttaaccag tatcaacgtc
     8701 tgtggcaacc ttcagccgca aaccgtcacc gtcagcgaac tggccgaacg ctgtttttgc
     8761 agcgaacgcc atgttcgtac gctgttgcgt caggcacagg aggcgggatg gctggagtgg
     8821 caggcgcagt caggacgcgg aaagcgcgga caattacgct ttctggtcac gccggaatcg
     8881 ctacgcaatg cgatgatgga acaggcactg gaaaccggaa agcagcaaga tgtgctggag
     8941 ctggcgcaac tggccccagg tgagctgcgc actctgttac agccatttat gggcggacaa
     9001 tggcaaaacg atacacccac gttgcgtatt ccctactatc gcccgctcga accgctacaa
     9061 ccaggctttt tgcccggccg tgccgagcag catctcgccg ggcagatatt ttccggcctg
     9121 acccgcttcg ataataatac tcagcgcccg attggcgatt tagcgcatca ctgggaaacc
     9181 tctactgaca ggttacgctg ggacttttat cttcgttcaa ccctacactg gcataacggc
     9241 gatgcagtaa aagcctcaca cttacaccag cgattattga tgctgttaca actgccagca
     9301 ctggatcaat tatttattag cgtgaagcgt attgaagtca cccatccgca gtgtctgacc
     9361 ttctttttac atcgccccga ttactggctt gcgcaccggc tggcgagcta ttgcagccat
     9421 ctggcgcatc cgcaattccc actgatcggc actggtcctt ttcgcttaac acaattcaca
     9481 gcggagctgg tgcgcctgga gagccatgat tattaccatt tgcgtcatcc gctgcttaaa
     9541 gcggttgagt actggataac tccgccgctt ttcgaaaaag atttgggaac cagttgtcgg
     9601 catcccgtgc aaatcaccat cggcaaaccg gaggagctgc aacgggtcag ccaagtcagt
     9661 agtggcatca gtttaggttt ttgctattta acgttgcgca aaagtccccg actctccctc
     9721 tggcaggcgc gaaaagtgat ctccattatt catcaatccg gtttattaca aacgttagaa
     9781 gtcggagaaa acctgatcac cgccagtcat gcattactgc taggctggac tattccgcac
     9841 tggcaagtac cggatgaagt caaactaccg aaaaccttga cgctggttta tcacctaccg
     9901 atagaacttc ataccatggc agaacgccta caggcgacac tggcagcgga aggctgtgaa
     9961 ctcacaatta tttttcataa cgcaaaaaac tgggacgaca cgaccctact ggcacacgca
    10021 gacctcatga tgggcgacag attaattggc gaagcaccgg aatatactct ggagcaatgg
    10081 ctacgttgcg atccactatg gccacatgtt ttcgacgctc cagcatatgc acatctgcaa
    10141 tcgacgctgg acgcggtgca agtaatgcct gatgaggaaa accgatttaa tgccctgaaa
    10201 gcggttttta gccagttaat ggcagacgcg acgctgacgc cgctgttcaa ctatcactat
    10261 cgcattagtg cccctcccgg cgtgaacggt gtgcgactga caccgcgcgg ctggtttgaa
    10321 tttaccgaag cctggcttcc cgcgccgtcg caatgaatag ctggtccggg ttaatcgcag
    10381 gcgttaccat aacgctttta ttgttcaagc aggattatct atgaaacgtg ctgtcgttgt
    10441 gttcagtgga ggccaggatt ccaccacctg tctggtgcag gcattacaac aatatgatga
    10501 agtccattgc gtgacgttcg attacggtca gcgacatcgc gcagaaatcg acgtggcacg
    10561 cgaactggcg ctgaaactgg gagcacgcgc gcataaggtt cttgatgtaa cgctgctcaa
    10621 cgagctggcg gtcagcagcc tgacacgtga cagcattccg gtgcctgatt atgaacctga
    10681 agccgatggc atcccgaata cgtttgtccc agggcgtaat attttgttcc tgacgctggc
    10741 ggcaatatat gcgtatcagg taaaagcaga agccgtaatt accggcgtct gtgaaacgga
    10801 tttctccggc tacccggatt gccgcgatga gtttgtgaaa gcactaaacc atgccgtcag
    10861 tttgggcatg gcgaaagata ttcgttttga aacgccgctg atgtggattg ataaagcgga
    10921 aacctgggcg ctggcagatt attacggcaa actggattta gtccgtaacg aaacgttgac
    10981 ctgctataac ggcattaaag gcgacggttg cggtcattgt gcggcatgta atttacgcgc
    11041 caacggtttg aatcattatc tggccgataa accgacggtg atggcagcga tgaagcagaa
    11101 aaccgggttg aggtaattat tccgggtgtc gccggatgcg gcttgagcat ccggcaccac
    11161 aaaacgttta cttaaccatc tgctccagct tttcgcgcaa ttccccttcc agagctaatg
    11221 ctttctgcgt tttaagatca atacaaacaa acgtaataag cgcatccgct accacctgcc
    11281 cttccggctc cagtgtaatg acctggttta agatgccgct tttaccgttt aattgctgca
    11341 actgactggt aatagttaac aggtcgctta ataccgctgg gcgacgatag ttaatattga
    11401 tattgaccac gacgaaggcg atgttatggg ccgtcatcca ctgaaaactg tcgctatttt
    11461 ccaccccatc ccagcgggct tcttcgagaa attcaaggta gcgggcgttg ttgacgtgct
    11521 ggtaaacgtc gagatgatat ccacgaactt tgatttgtgt ttgcatagcg caataacctt
    11581 acgttgttgt tataagtaca gaggtcataa ctggtatgac ctcttcagtc tggcaaaaaa
    11641 ttgccactat gcaaattaat tacagggtta ataccgccag attacgttcc accagcgaat
    11701 tgcccatccc cggcacctgc tttagatcct ccaccgtttt aaacggaccg tactcttcgc
    11761 gataactgac aatcgcctgc gctttcttca ggccaacgcc attcatcgcg cgggctagct
    11821 cttccgcgct ggcattatta atgctgaccc gggtgccttc ttcgtcactg gctttcgccg
    11881 gtactgctgc tttactttgt gctgcaggag cttccgcttt ggtttctacc gccgtcggtt
    11941 tcgccacaga agctgccgcc agcgcgctat gagacattcc ggcacaggcc agggacaggg
    12001 taatgagcag tgctttaatt ccgtgtttca tactgttttc tccttgtttg ttaacagtgg
    12061 aatcacctta acgggagtga aaacagtaaa caaatggcaa tgttcagaaa tggaaaaggc
    12121 cgcgaaagcg accttttctt gttacagctg caacgcattt ttttgcgagg cggcttcagg
    12181 attattgctg ttccagcgca tcgccaattt tgattttcgc ctctttacgc aggttactca
    12241 tcagagcttc aaagacgatt tgtgcgttgt tctgggtgat accctgcacc atcgcttttt
    12301 tctgatcttc cggcattgaa ccttgtttca cttcatccag cgccagcaga accacattac
    12361 cttgcatatc ggtcgccata ccgtagctcg gtttgtcttt cgctggcagt ggcagtgcaa
    12421 acgccgcctg gctaatcggg tcacgaccgg aacggcttaa ggttttcggc tcgccaaatt
    12481 tcagaccggc agcctgcata gcttccgcac ctttgccggc tttcaaatca accagcagtt
    12541 tctcagcatc cactttcgcc tgttgttcag ctttgttgtg ctgaaccagt gccttaactt
    12601 gttcctgaac atctgccaac ggtttcaccg cttccggttt gtgctcgctg acgcgcagca
    12661 cgaatgcgcg gtcgccgtct acggtgatga tgtcagagtt gatgcccgac gcgccgtttt
    12721 cacctaccag accgccgtta aagatagcgt cggcaaccgg cttgaagttc aactcttccg
    12781 gcaggttatc tttgctgaac caacccgtct gagtggcttt aacgccggca gcttgctctg
    12841 caccggccag agactcggtg tcgttgcttg ccgcatcgct cactttctgc tgcagcgcat
    12901 agtacgcatc gagggctttt tcgtgtttca ctttcgccgc aatgtcgtca cgtacttcgt
    12961 ctaacgattt cactttcgct ggctgaatgt cgtccagacg tacaatcagg aaaccgaccg
    13021 aagatttgat gacaccagac agttggcctt tttctttcag accagcattt ttcagttcgt
    13081 cggggatagt ggcatcttct aaccaaccca tatcgccgcc gttacgggca gagataatat
    13141 cggcagattt ttctttggct aatgcagcaa aatcaccgcc tttattcagc tcatcaagta
    13201 ccgctttcgc ttcatcttca gttttggtct ggatgatgct gtagcgggta cgctgcggct
    13261 gggtgaattg atcctgatgc tgatcgtagt agctctggat atccgcatcg ctaaccggtt
    13321 gctgcatcgt tgcggcaacc agcttgatgt aactcacgcg gaattgttcc ggcgtcatga
    13381 aattgttttt gttttgttcg tagtagctgg caatctcctg ttcggtcaca ggctgcttcg
    13441 ccgccagcgc gttaacatcg atagtcgcct cacgcaccac gcgttgttgc gcgaccagtg
    13501 ccgccagctc gtcggtttca cctttcagca taaaatcggt accggcaacg ccgttaatca
    13561 gctgttgggt agtgagctgg ttacgcagcg cctgggcgta ctgatcggcg gtcatcccca
    13621 tctggttgag gataccgtta tagcggctgt tatcaaattt gccgtcaacc tggaaggctg
    13681 gggtcgcgaa aatcgcctgt ttaacctgct catcactgat acccagtttc agctcacgag
    13741 cgtactgatc caacagcgcc tcgtcgatca gacgattcag cacctgttga cgcagggttt
    13801 tcatatagcc ttcgttcgct gccagctcgg agtattgatc gcccagctgt tgctgcatac
    13861 gattacgctc gctgttaaag gcgttctcga actgcccacg gctgatttcc tggtcattca
    13921 cttttgcggc gtagttattg cctccgccaa tcaggtaacc actcacgccg gtcaatatga
    13981 acgacacgat aatgataccg aaaataatct tgagcacgag actgtttgca gccgtgcgta
    14041 agctgtccat catggtgtaa caacactccg ctgtagatga ctgtgtacct cacgcaacgt
    14101 atcgatgcgc gcaaaggcct attgtgacaa gaaacggggg caattgtcag cccacaagac
    14161 acagaaagtc accgaataca aataaaaaag gcacatcagt agatgcgccc ttgaacttcg
    14221 tcacatcccc actggggaca acgcttagtt taccgcgtct ttcagtgctt tacctgcacg
    14281 gaagctcggt actttagcgg cagcgatggt gatctcttta ccggtctgcg ggttgcggcc
    14341 agtacgggca gcacgctctt taacggcaaa agtaccaaaa cctaccagtg ctacatcatc
    14401 cccttctttc agagattcag ttacggaagc aataatagca tctaacgcac ggccagccgc
    14461 agctttagag atatcagccc ctgcagcaat cttgtcgatc aattgagatt tattcactct
    14521 tctcttcctc tttataattt atatcgcact tgaatccttc aaggtacgaa cgcgcagcag
    14581 ttatatcagg ccagccatcc ccttacaaca ccccttaata agggcaagcc cgatccgcta
    14641 tctaacttag cgagacaaaa aaaggctggc aagcccgaat tagcctgcca gccctgtttt
    14701 tattagtgca ttttgcgcga ggtcactatt ttgcagtcac aacctgcatg ccagacggtt
    14761 cattttgcag cgccagagtc agaacttcct caatgcgctt cacaggatga atgtccagat
    14821 cggcaattac gttgtcagga atctcttcca gatcgcgttt attttcgaac ggaattagca
    14881 ctgttttaat cccgccgcga tgcgctgcca ggagtttttc tttcaaacca ccgatcggca
    14941 gtacctgacc acgcagagtg atctcaccgg tcattgccac atcggcacga accgggttac
    15001 cggtcaggca agaaaccagc gcggtgcaca tagcaatacc ggcactcgga ccatctttcg
    15061 gcgtcgcacc ttccggtacg tggacgtgga tgtcacgttt ttcgtaaaaa tcagggttga
    15121 tccccagttt ttccgcacgc gcacgaacca ccgttaacgc cgcctgaata gactcctgca
    15181 tcacttcgcc gagcgaaccg gtataggtca gtttgccttt acccggaaca catgcggttt
    15241 caatggtcag caagtcaccg cccacttccg tccacgccag accgattacc tgaccgacac
    15301 ggttttcgtt atcagcgcga ccatagtcga aacgctgaac accgaggtag tcatgcaggt
    15361 tatcgccgtt aatttcgata tgttttaatg acttatcgag cagtaactgc ttaaccgctt
    15421 tgcggcacag tttggagatt tcacgctcca gaccacgcac gcccgcctca cgggtgtagt
    15481 aacgaataat gccgataatg gcgctatcgt cgacggtcag ctcacctttt ttcagtgcat
    15541 tacgttcaat ctgcttcggc agcaggtgac gtttggcgat gttcagtttt tcatcttcgg
    15601 tataaccgga gaggcgaatc acttccatac gatccagcag cggtgccgga atgttcatgg
    15661 agttcgacgt cgcgacaaac atcacgtcgc tgagatcgta atccacttcc aggtagtggt
    15721 cgctgaacgc tacgttctgc tctggatcca gcacttcaag cagtgcagag gccggatcgc
    15781 ctcgcatgtc agaagacatt ttgtcgatct catcgagcag gaacagcgga tttttcacgc
    15841 ccactttcgc cattttctgg atcagtttac ccggcataga accgatgtaa gtacggcggt
    15901 gaccacggat ttccgcttca tcacgcacgc cgcccagcgc catacggaca tatttacgcc
    15961 cggtggcttt ggcaatggac tgaccaagag aggttttacc tacccccggc ggccctacca
    16021 ggcagaggat cggtcccttg attttgttga cacggctttg aaccgcaagg tactcaagga
    16081 tgcgatcttt cacgcgctcc agaccataat ggtcggtatc aaggatttcc tgcgcctgac
    16141 gcaggtcttt tttgaccttg ctgcgcgcat tccacggtac ctgtaccatc cagtcgatat
    16201 aaccacgcac tacggtcgct tctgccgaca tcggagacat cattttcagc ttctgcaact
    16261 ctgcttccgc tttctctttt gcctctttcg gcatcttcgc cgcgtcgatt ttgcgcttca
    16321 gggcttcgtt ttcgtccggc acgtcgtcca tttcaccgag ttctttctga atagctttca
    16381 tttgctcgtt cagatagtac tcacgctggg atttctccat ctgcttttta acgcggttgc
    16441 gaatgcgttt ctcaacctgc agcagatcga tttccgattc catcattgcc atcagatatt
    16501 ccagacgttc gttaacgtcg gacatctcca gaacggactg tttgtcagcc agtttcagcg
    16561 gcatatgtgc agcaatggta tccgccagac gcgccggatc gtcgatgcta ttcagcgacg
    16621 tcagcacttc tggtgggatt tttttgttca gcttgatgta gccttcgaac tggctgattg
    16681 cagtacgcac cagcacttcc tgttcccgct catcaatggt cggcgactcc agatactccg
    16741 ccttcgcaga aaagtgttcg ccattgtcag agagcgcaga aatacgcgcg cgctgtaacc
    16801 cctcgaccag cactttgacg gtgccgtcag gcagtttcag catctgcaat atagaggcca
    16861 cggtcccgac ggtgaaaaga tcgtttacac ccggctcatc cgttgaagct tctttctgcg
    16921 cgaccagcat aattttttta tcatggtcca tcgccgcttc cagacaacgg atagattttt
    16981 cccgcccgac aaataagggg atgaccatgt gcggataaac caccacatcg cgcagcggca
    17041 atacggggat ttcaatgcgt tcagaacgct caggattcat agagctctct cttagtttaa
    17101 tttccgccag gtaatcagat gacacgactg tgcttcacgc catctattaa catgtacgtc
    17161 agtatatggg gatgtttccc ccacattcaa cgccgagaat agaggaaaaa ttaaagggga
    17221 gataaaatcc cccctttttg gttaactaat tgtatgagaa tggttaatta ttcaccagat
    17281 gcctgttgcg cttccggctt accataaatc agcaacggtt tgctttgacc atcaattacc
    17341 gactcgtcga taaccacttt ttcgacgtct tccatggacg gcagatcgta catggtatcg
    17401 agcagtgcgg cttctacgat ggaacgcagg ccacgggcac cggttttacg cgccatcgct
    17461 ttcttagcga tagcatccag cgcctcgtca cggaattcca gatccacgcc ttccagatta
    17521 aacagcgcct gatactgctt ggtcagggcg tttttcggct ctttgaggat ctgaatcaga
    17581 gcttcttcgc tcagttcatt caacgttgct acaaccggca gacgaccaat gaactcaggg
    17641 ataagaccaa acttgatcag atcttccggt tcaacctgcg ccagcagctc gccttcgctt
    17701 gctttgtcgg acttcgcttt taccgtcgcg ccaaaaccaa tgccggagcc ggtttctaca
    17761 cggtgggaaa tcactttatc cagaccggca aacgcaccgc cacaaataaa caggatctta
    17821 gaggtatcaa cctgcaagaa ttcctgctgc ggatgtttac gcccaccttg cggtggaaca
    17881 gcagctaccg taccttcgat cagtttcaac agtgcctgct gtactccttc accggaaacg
    17941 tctcgggtaa tggacgggtt gtctgactta cgagaaatct tgtcgatttc atcgatgtag
    18001 acaatcccgc gctgcgcttt ctgtacgtcg taatcgcact tttgcaacag cttctgaatg
    18061 atgttttcaa cgtcttcgcc cacataaccg gcttcggtca gcgtggttgc gtcggccatg
    18121 gtgaacggga cgtccagcag gcgcgccagc gtttcagcca gcagcgtttt accggaaccg
    18181 gtcggaccga tcagcagaat gttacttttg cccaactcga caccattgct ggtatcgccg
    18241 ttgcgcagac gtttgtaatg gttgtatacc gcgaccgcca gcactttttt cgcctgttcc
    18301 tggccgataa cgtaatcgtc caggtggttg cgaatttcat gcggcgtcgg tagcgcactg
    18361 cgttcacgat gcggtgcaac ttctttaatc tcttcgcgaa tgatgtcgtt acataaatca
    18421 acacattcgt cgcagatata cacggatgga ccggcaatca gcttgcgcac ttcatgctgg
    18481 cttttgccgc aaaaagagca atacagcaat ttgcctgagc catctttgcg tttatctgtc
    18541 atgggtcaaa acctcttctt tgttctttgt gccgcacacg acgacgcaaa tgccattctc
    18601 aggcgcaagc cgcttacagc gttgtgccgc cctagataag tatagcggca cagttgcgcc
    18661 tctggcatca attacgatgg gtcagaatcg aatcgaccag accgtattcc accgcttcag
    18721 gggcggaaag gaagcgatcg cgctcggtat cacgttcaat ctgttctaat gattgacccg
    18781 tatgaagcgc cataagttca ttcatgcgcc ctttaacttt cagaatttca cgggcatgaa
    18841 tttcgatatc ggtcgcctgg ccctggtagc cgcccaacgg ttggtgaatc atcacgcgcg
    18901 aattcggcag gcaaaaacgt ttaccttttg cccctgcggt caacaagaaa gcgcccatcg
    18961 aggccgcctg gcccatacag atggtgctga catcaggctt gataaactgc atggtgtcat
    19021 agatagacat cccggcagtg atcaccccgc ctggggagtt aatgtacaga tagatatctt
    19081 tttctgggtt ttccgcttcc aggaacagca tctgcgccac aatcaggtta gccatgtggt
    19141 cttcaacctg gccagtcaga aaaatgacgc gttccttaag tagacgagaa tagatatcaa
    19201 aagagcgctc accgcgtgag gtctgttcaa tgaccatcgg caccagcgcc atatggggtg
    19261 caaagttatc tcgttcgccg ctgtatgaca tttccgtctc ctggataaaa ttgaaaaaaa
    19321 cctgctgtac cgattgtaac cttatggacg gattatcagc tagtccctgt gttatgggta
    19381 cggcatcacc atatttcaag cataacaatc ttttgttgtt acgctaacac cgaaagaggc
    19441 ttttcgcacg gctccatgca aaattcatga caaaaaaaag cccaccacct ggcggtgacg
    19501 ggcctttgtg cgaatttagc gcgttatgct gcgtaaatta cgcctgctgg ttcatcagct
    19561 cgttgaaagt ggtttctttt tcagtcactt tcgctttcgc cagtacagct tcaacagcct
    19621 gttcttccag agcaacattg cgcatgttgt ccatcagttc tttgtttttg ctgtagaact
    19681 cgataacttc tttcggatct tcgtacgcag aagccatctc ttcgatcagg cctttcacgc
    19741 gctcttcgtc agctttcagc tcgttggtgc ggataacttc gcccagcagc aggccaacaa
    19801 ctacgcggcg tttagcctgt tcttcgaaca gttcgcgcgg cagttccaga gcttgttttt
    19861 cgttgccacc gaaacgctgt gcagcctggc gacgcagaac gtcgatttcg ctgtcgatca
    19921 gcgcagccgg tacgtcgatg tcgttagctt ttaccagacc ttcgatcgcc tgagacttaa
    19981 cgcggttacg gatggcgctc ttcagctcgc gctccatgtt tttacgcact tcagcgcgca
    20041 gaccttctac ggaaccatct tcaacgccga aacgtttgat gaattctgca gtcagttccg
    20101 gcagttcacg ctcttcaact ttcttcaggt tgatagcgaa tttcgctgct ttacctttca
    20161 ggttttctgc gtggtattct tccgggaagg tcacgtcgat ggtgaactct tcgccagctt
    20221 tgtggccttt gataccgtct tcaaagcccg ggatcatacg accctggccc atcgccagta
    20281 cgaaatcaga cgctttaccg ccttcgaact cttcgccgtc tacagaaccg gtgaagtcga
    20341 tggttacgcg gtcttctgct tcaacagcgc cgtctttttc tttccaggtc gcctgctgtt
    20401 tacgcagagt atccagcatg ccgtcaacgt cagcgtcggt cacttcaacg atcggttttt
    20461 caacttcgat cgcttccaga ccctgcagtt caacttccgg ataaacttca aactctacag
    20521 agtaagtgaa gtcttcaccc agcttgtatt cgcccggaac ataagtcggt gcgccagccg
    20581 gattgatttt ttctttaatg atggcgtcaa tgaagttacg gctcatcagg tcacccagaa
    20641 cgtcctggcg tacagacgcg ccataacgct gagcaacgat attcattggc actttgcctt
    20701 tgcggaagcc gtcaatacgt acttttttcg caacgttgac cagctcgctt ttaacagcgg
    20761 tctcgatgct gtcagcagcg atagtaatcg ttacacggcg gccaaggcct tgagtggttt
    20821 caactgaaac ttgcatcttg ttacctcaaa aaatcacagt gctcggtcaa ctcttacccc
    20881 gcaagcacag gttgttggct tcgcggaacc gggatgttct tataatcaat cacattccct
    20941 gttaccagaa tcatcccgaa agcattcaaa taggacgcgg cattatagcg gcatcacttt
    21001 tatgagtcga gaacggttat tgcgcgctgt cgcgtatttt ttcacaattc ccgggcaatt
    21061 ttcgtctgaa tgctgggcaa aaagcgcaaa atccagacaa aacaaaacgg cccgcaggcc
    21121 gtttttcata aaatctttag caacatactg gaaaagcgcc gacagttgca aatgcgtttt
    21181 tacgcgatgc ttcctgctcc acgacaggga ggagaggcaa agacggtgtc ctgcaaccct
    21241 tcccactcct taatagtgta agtatgcaga gccagcgcat gaacggtagt agagagttcc
    21301 tccgctaaag tactgtaaat cattcgatga cgattcagaa aacgttcacc cgtaaaacga
    21361 tcgctgacca gcacaacttt aaaatggctt tcagagccgg ctgggacatt gtgacgatag
    21421 ctttcatcca ctacttcgag gaatacgggt tggaacgccg cccttaattt ttcttctatc
    21481 cgctcacgta tcatcatgaa atatctcctc cgacaacgct gagatgtcat ccatcccttt
    21541 aaatactagc cgcttttacc gtttccattg cagcttaaca acaaatattt aggtttcgtc
    21601 ctggtttttc cgtcaagcgt atgaagttta ctgatttaaa gattgaaaca caggcctcgc
    21661 atcagcgttt cttttaccag gggtgattat ttcgtcaaca tgatgaattt acatgcctta
    21721 cccacttccc ctcgccgttg gcaatgttat gatggcggga atttttacca aacacgctta
    21781 agagtctctg agagcccgaa catgttcaaa aaaatcctct tcccgttagt tgctctgttt
    21841 atgcttgcag gatgcgcaaa accgccaaca actattgaag tttccccgac gattacactg
    21901 ccacagcagg atccaagcct gatgggcgtc accgtaagca ttaatggtgc cgatcagcgt
    21961 accgatcagg cgctggcaaa agtcacccgc gataatcaaa tcgttaccct gaccgcctcc
    22021 cgcgctctgc gtttcctgct gcaagaagtg ctggaaaaac agatgaccgc gcgtggttac
    22081 atggttggtc cgaatggccc ggttaatctg caaatcatcg ttagccaact gtatgctgac
    22141 gtgtcccagg gcaacgtgcg ctacaacatc gctaccaaag cggatatcgc catcatcgct
    22201 accgcgcaga atggcaacaa aatgaccaaa aactatcgtg ccagctacaa cgttgaaggt
    22261 gcgttccagg cctccaacaa aaatatcgcc gatgcggtta acagtgtgct gaccgatacc
    22321 atcgctgata tgtctcagga caccagcatt cacgaattca tcaagcagaa cgcgcgttaa
    22381 tttctgccct ctggcccggt gcaagccggg cctgtagacg cccatgtcca gtcaatattt
    22441 acgtattttt caacagccgc gttcagccat attgctgatc ctgggttttg cttccgggct
    22501 accgctcgcc ctgacatccg gcaccttaca ggcctggatg acggtcgaaa atatcgatct
    22561 caaaaccatt ggtttcttct ctctggtagg ccaggcttac gtttttaaat tcctctggtc
    22621 accgctgatg gaccgctaca cgcccccatt ttttgggcgg cggcgcggtt ggctgctcgc
    22681 cacgcaaatc ctgttattag tcgccattgc ggcgatgggt tttctcgaac caggcaccca
    22741 actccgctgg atggcggcgc tggcggtggt gatcgctttt tgctctgcct cccaggatat
    22801 tgtcttcgat gcgtggaaaa ccgatgtgct cccggcagaa gaacgtggtg cgggcgcggc
    22861 aatcagcgtg ctgggttacc gtttagggat gctggtttcc ggcggcctag ccctgtggct
    22921 ggcagataaa tggttgggct ggcagggcat gtactggttg atggcggcgc tgttgatccc
    22981 ctgtattatc gcgacgttgc ttgcaccaga accaaccgac accattcctg tgcccaaaac
    23041 gctggaacaa gcggttgttg cacctctgcg agatttcttt ggtcgcaata atgcctggct
    23101 tattttgctt cttatcgtgc tgtataagct gggcgacgca ttcgccatga gcctgacaac
    23161 cacgtttttg attcgcggcg tcgggtttga tgcgggtgaa gtaggcgtgg taaacaaaac
    23221 gcttggctta ctggcgacca ttgttggcgc attgtacggt gggattttga tgcagcgcct
    23281 gtcgctgttc cgggcgctgc tgattttcgg cattttacaa ggtgcgtcta acgctggtta
    23341 ctggctgctg tcgattactg ataagcatct ctacagcatg ggcgcagccg tctttttcga
    23401 aaacctctgt ggcgggatgg gcacatcagc ctttgtcgcg ctgttaatga cgctgtgtaa
    23461 taagtcattt tccgctactc aatttgccct gctctcagcg ctttccgctg tagggcgagt
    23521 ttatgtcggc cccgtggcgg gttggtttgt tgaagcacac ggctggtcga cattctatct
    23581 attctccgtc gccgctgccg taccggggct tattttgctg ctggtttgcc gccagacgct
    23641 tgaatataca cgagtaaatg acaactttat ctcccgtacc gaatatccgg caggttatgc
    23701 ctttgccatg tggacactgg cggcgggcgt cagcctgttg gccgtgtggt tacttctgtt
    23761 gacgatggac gcgctggatt tgacgcactt ctctttcctg cctgctctgc tggaagtcgg
    23821 ggttttagtc gccctttctg gcgtcatgct tggtggtttg ctggattatc tggcgctacg
    23881 aaaaacgcat ctgacgtaat ctgtaaatat tatttagctg tagtaatcac tcgccgtaat
    23941 tattacggct aaataaacat cagcatcgct tattaatatt tgttcttttg tgcggcttag
    24001 cgtttggaaa ttattgacgc catttataaa ttctattttt cttacacgat tcagctaatg
    24061 agtctttatt ttctcaccac ccagttgtca ctttaatgat aattatttgt taaataattg
    24121 ttttatttcg cattggttat accaattgcc cgccaagact ccttctgcac tccccttttg
    24181 tta
//
LOCUS       DAEEUE010000059        22624 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.059, whole genome shotgun sequence.
ACCESSION   DAEEUE010000059 DAEEUE010000000
VERSION     DAEEUE010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22624
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.059"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..435)
                     /gene="pgrR"
                     /locus_tag="KLK84_002495"
     CDS             complement(<1..435)
                     /gene="pgrR"
                     /locus_tag="KLK84_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HBE2009783.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGV"
     gene            573..1493
                     /locus_tag="KLK84_002496"
     CDS             573..1493
                     /locus_tag="KLK84_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBE2009784.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1493..2430
                     /locus_tag="KLK84_002497"
                     /pseudo
     CDS             1493..2430
                     /locus_tag="KLK84_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            2448..3086
                     /locus_tag="KLK84_002498"
     CDS             2448..3086
                     /locus_tag="KLK84_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBE2009785.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            3377..4105
                     /gene="mpaA"
                     /locus_tag="KLK84_002499"
     CDS             3377..4105
                     /gene="mpaA"
                     /locus_tag="KLK84_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HBE2009786.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(4080..5039)
                     /gene="ycjG"
                     /locus_tag="KLK84_002500"
     CDS             complement(4080..5039)
                     /gene="ycjG"
                     /locus_tag="KLK84_002500"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HBE2009787.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALATEARAQGFSLMLGCMLC
                     TSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            5158..5664
                     /gene="tpx"
                     /locus_tag="KLK84_002501"
     CDS             5158..5664
                     /gene="tpx"
                     /locus_tag="KLK84_002501"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HBE2009788.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(5708..7249)
                     /gene="tyrR"
                     /locus_tag="KLK84_002502"
     CDS             complement(5708..7249)
                     /gene="tyrR"
                     /locus_tag="KLK84_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HBE2009789.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(7397..8458)
                     /locus_tag="KLK84_002503"
     CDS             complement(7397..8458)
                     /locus_tag="KLK84_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HBE2009790.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISHSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(8455..9852)
                     /locus_tag="KLK84_002504"
     CDS             complement(8455..9852)
                     /locus_tag="KLK84_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HBE2009791.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            10008..11006
                     /locus_tag="KLK84_002505"
     CDS             10008..11006
                     /locus_tag="KLK84_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBE2009792.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADKSFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(11117..11989)
                     /gene="ompG"
                     /locus_tag="KLK84_002506"
                     /pseudo
     CDS             complement(11117..11989)
                     /gene="ompG"
                     /locus_tag="KLK84_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(12062..13143)
                     /locus_tag="KLK84_002507"
                     /pseudo
     CDS             complement(12062..13143)
                     /locus_tag="KLK84_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(13157..13816)
                     /gene="pgmB"
                     /locus_tag="KLK84_002508"
     CDS             complement(13157..13816)
                     /gene="pgmB"
                     /locus_tag="KLK84_002508"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBE2009793.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(13813..16080)
                     /gene="ycjT"
                     /locus_tag="KLK84_002509"
     CDS             complement(13813..16080)
                     /gene="ycjT"
                     /locus_tag="KLK84_002509"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HBE2009794.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(16077..16700)
                     /locus_tag="KLK84_002510"
     CDS             complement(16077..16700)
                     /locus_tag="KLK84_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBE2009795.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(16697..17119)
                     /locus_tag="KLK84_002511"
     CDS             complement(16697..17119)
                     /locus_tag="KLK84_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBE2009796.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSRLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(17141..17916)
                     /locus_tag="KLK84_002512"
                     /pseudo
     CDS             complement(17141..17916)
                     /locus_tag="KLK84_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(17934..18986)
                     /locus_tag="KLK84_002513"
     CDS             complement(17934..18986)
                     /locus_tag="KLK84_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBE2009797.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(19017..19859)
                     /locus_tag="KLK84_002514"
     CDS             complement(19017..19859)
                     /locus_tag="KLK84_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBE2009798.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(19846..20727)
                     /locus_tag="KLK84_002515"
     CDS             complement(19846..20727)
                     /locus_tag="KLK84_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBE2009799.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWITVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(20748..22031)
                     /locus_tag="KLK84_002516"
                     /pseudo
     CDS             complement(20748..22031)
                     /locus_tag="KLK84_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(22045..>22624)
                     /gene="ycjM"
                     /locus_tag="KLK84_002517"
     CDS             complement(22045..>22624)
                     /gene="ycjM"
                     /locus_tag="KLK84_002517"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HBE2009800.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5574 a         6085 c         5514 g         5451 t
ORIGIN      
        1 gacgcctgca tcaaaacgtt cagaaacgac atcggtcaaa ccataatcaa tggtaagctg
       61 aatatcaatt tcaggatgcg atttcaggaa tgtgcgcatg gctggtaaca atatcgtttt
      121 tgctgcatgt tctacagtag taatacgtat tgtcccggat gggcggttct gcagatcgct
      181 cagggatgtc agggcagaat ctatgtcatg caatataggg ccaagaacag acaaaagatg
      241 ctcgcccgct tcagtgggta caacgctgcg cgtggttcgc gtcagaagcc gcaatcccaa
      301 tcgttcttct atacggcgca ctatctggct taaagctgac tgcaccatgc tcaggcgggc
      361 tgctgcgcga gtgaagctac gctcctctgc aacgacgaca aacgccatca gatcagcgat
      421 ttcttcacgc ttcattcagt atctccatac aatctattaa tcatttaaat gataggtcta
      481 atcatgattt agggtctaat caacttaatg atcaatatct atagtgatat caccaaccgt
      541 caaaacgaac cgacaactcc atgaggaact gaatgatgaa taataaagtc agcttcacta
      601 acagcaataa tccaaccatc tctttgtctg cagtgatcta tttccctcca aaatttgatg
      661 aaacccgtca gtatccggca attgtggtat ctcacccagg tgggggcgtt aaagagcaaa
      721 cggccggaac ctatgccgaa aagctggcgg aaaagggatt tgtcactatt gcttatgacg
      781 catcttatca aggtgaaagt ggcggcgagc cgcgtcagtt agaaaaccca tacattcgta
      841 ctgaagacgt cagtgccgtg attgattacc tgacaacgct ttcttatgtc gacaataccc
      901 gaattggtgc gatgggaatt tgtgccggtg caggatatac agccaatgct gcaattcagg
      961 atcgccgcat caaggccatc ggtaccgtta gtgcggtcaa tattggttcg atgttccgca
     1021 atggttggga aaacaatgtg aaatctattg atgctctgcc acacattgaa gctggctcaa
     1081 acgcccgaac cagcgatatt agcagcgaag aatacgccat tatgccactg gctccaatga
     1141 aggaggccga tgcgccgaac gaagaactgc gtcaggcctg ggagtattac cacacacctc
     1201 gtgcgcagta tccaacagca ccgggttacg ctactttgcg cagccttaac cagattatta
     1261 cctatgatgc ttaccatatg gcggaagtgt acctgactca gccgatgcag attgtggcgg
     1321 gcagccaggc agggagcaaa tggatgagtg acgacctgta cgatcgggca tcaagccagg
     1381 ataaacgcta tcacattgtt gaaggcgcaa accatatgga tctttatgac ggcaaagcct
     1441 atgtggctga agcaatttca gtattagctc cgttctttga ggaaactctg taatgaaaca
     1501 tattcaaatc cgcaatagtg atatggcctg gcatattgct gccaatattc agtttccacc
     1561 aaattttgat gagtcaaaac agtatccggc gattatcagc gtccatcctt ttggcagttg
     1621 taaggagcag acgtctggca atatctatgg caaggcattg gctgaaaaag gctatgtcgt
     1681 tctcgcttat gacgccagtt tccagggaga atctggtggg tagcctcgtt ggatagaaga
     1741 tccgacacag cgcgtagagg atattagccg tgttatcgat tatgcagtaa ctctgccata
     1801 tgttgatgct gaacgcattg gcgtgctggg tgtgtgtggg gggggggggg tggctatgcc
     1861 attaatgccg cgctgacaga aaaacgaatt aaagctgtag tgagtattac gggtgttaat
     1921 atcgggcgtc tttttcggga aggtttcagc aattatgatc cgattggcgt attgaatgca
     1981 atggcgtcac aacgagcaaa agaggctcgt ggtggagaat tacagatcaa cgaattactt
     2041 cccgccagcc tggatgcagc aaaagcccat ggactgaccg aacgcgatgt ttatgaagca
     2101 actgactact ataaaacacc acgcgggcaa caaccgggag gtgcgacaaa aatgttgttc
     2161 tcacatgcac agaaaacgct ggcctgggat gccttcgcat ttactgaagt gttactgacc
     2221 cagccggtga tggtagtagt tggtgaaaaa gtcggtgcct tcggtgctta tcgtgatgga
     2281 ctggaagtct atggccgggc gatggtttcg caggatcgtc agttagtttc gttacccgat
     2341 ttctcacatt atgaactata tgacaaaccg gaagcagttc aggaggcgct ggcaaaagtt
     2401 attccttttt tcaacaccca tctgggataa gtttcatgag aaatattatg aaaaatgtac
     2461 tgattcttgg tgctggcggt cagatagccc gccatgtgat taatcaactg gcagataaac
     2521 aaactattaa acaaacttta tttgcccgtc agccagcaaa aatacacaaa ccctatccaa
     2581 ctaatagcca gattattatg ggggatgtac tgaatcatgc agcactgaag caggccatgc
     2641 agggacagga tattgtgtat gccaacctta cgggggaaga tttagatatt caggcaaatt
     2701 cagtgatcgc tgcgatgaaa gcctgtgatg ttaaacgtct gatctttgtt ctttccctcg
     2761 gtatttatga tgaagttcca ggaaaatttg tcgaatggaa taatgcagtt attggtgaac
     2821 ccctaaaacc attccgcagg gctgcggatg caattgaggc ttctggactg gaatatacaa
     2881 ttcttcgccc tgcctggctg actgatgaag atattattga ttatgagctt acctctcgca
     2941 atgaaccatt caaaggtacg atcgtatcac gtaagagtgt cgcagcatta attacagata
     3001 tcatcgataa acctgagaaa cacattggtg agaatatagg catcaatcaa cccgggactg
     3061 atggcgataa gccattcttc atgtaaatca attatataga taccatcgta ctgtactatg
     3121 tgcagtacga tttattaaac ttattaaggt gaactgatca atcagatgcg attaacatgt
     3181 gtagaaccag taagaataaa atacgccatg tatataacat tacgacaatt ctaatatctg
     3241 cacatcaatg aaaatgcaaa caacttccgg caaaaaagtg atacaagatc gatgacatag
     3301 cgttgaagaa aaggatttga taatccgcaa aatttgcaca acactgcctg taatacttcc
     3361 tgaaccagag acgatcatga ccgtaacccg ctcacgcgcc gaacgcggcg catttccgcc
     3421 cggaacagaa cattacggac gttcattatt gggcgcgccg ttaatctggt ttcctgcccc
     3481 tgcagccagc cgcgaaagtg gtttgattct ggctggcact cacggtgatg aaaactcttc
     3541 ggtcgtcacg ctctcctgtg ctctgcggac tttgacacct tctttgcgcc gccatcatgt
     3601 ggtgctgtgt gtgaatcctg acggctgcca gctggggtta cgggccaatg ctaatggtgt
     3661 ggatttaaac agaaactttc cggcggcgaa ctggaaggaa ggtgaaacgg tttatcgctg
     3721 gaacagcgcc gctgaagaac gtgatgtcgt attactgaca ggtgacaaac ctggctccga
     3781 acctgaaacc caggcgctgt gccagcttat acaccgcatt caacctgcct gggtggtctc
     3841 attccacgat ccactggctt gtattgaaga tcccagacac agcgaattag gcgaatggct
     3901 ggcccagtcg tttgaattgc ctctggtaac cagcgtcggt tatgaaacgc ctggctcttt
     3961 cggcagttgg tgtgcagatc tcaacttgca ttgcatcacc gcagagtttc cgcccatctc
     4021 ctccgacgaa gccagcgaaa aatatctctt tgcgatggca aacttgctgc gctggcatcc
     4081 taaagatgca attcgcccgt cgtgaactga agcgccggtt ccacatctac cgccagccag
     4141 gtcggtccgt caagatcggc gaaactgacc tgcggcacca gcggtaaagc ggcgctaatg
     4201 gcacgagagg tacacaacat gcagcccagc atcagactga aaccttgtgc acgcgcttca
     4261 gtcgccagcg cttccgtcag accaccggtt ttatcgagct taatgttaac catctcatag
     4321 cgccctttca gcgccttcag attgctacga gtatgacaac tttcatcagc acaaatcggc
     4381 aacggatgaa taaaattctc cagcgccgca tcgtcctgcg caggaagcgg ttgttcaagc
     4441 atcgcaacgc ctaaatccgc caatagctgg caacgcgccg ccaacccttc tgcacgccag
     4501 gattcatttg catcaacgat cagcgtggca tcgggcacag cggtgcgaat tgccaccatc
     4561 cgctcactga taagatggtt atccagcttc actttcagta atttcgcgcc tgcctgccag
     4621 agtgttgatg cactattggc catctgatca ggcgtaccga tgacaaccgt ctgtgcagtg
     4681 ataactgtct cgggaagcgt tatgccgatc aaatcagcca gcgattgctg ctgttttcgc
     4741 gccgcgagat cccacaatgc acaatccagc gcattacgtg ctgcgccggc agggagaatt
     4801 ttttgcaact cctcccgtgt cagccctttc tctaattgcg gcacgacgct cataatttgc
     4861 gccattaccg aggcatcact ttctccataa cgcggatacg gcgtgcattc gccggtgcct
     4921 ttaataccct cttcttccag ttcaaccacc accacgcgcg cttcactgcg acttccccgg
     4981 gcaatcacaa acggggtatg taagggccag gcttcctcaa ataccttaac gattctcatc
     5041 tttactcctt gttgtcgcgg tggtctgcgg attaagcgca aatagcgttt gctgtgttat
     5101 tgacagttag cataaactag gtgtgacgtt aactatatgt aaacaggaaa gataatcatg
     5161 tcacaaaccg ttcatttcca gggcaacccg gttacagtcg ccaattccat cccgcaggcg
     5221 ggtagcaaag cgcagacttt tactctcgtg gcaaaagatc tgtctgacgt caccctcggt
     5281 cagtttgcgg gtaaacgcaa agtgctgaac attttcccga gtattgatac cggtgtttgc
     5341 gccgcatcag tacgtaagtt taaccaactg gcaactgaga tcgacaacac cgttgtgctg
     5401 tgtatctctg ccgatctgcc gttcgcccag tctcgtttct gcggcgcaga aggtctgaac
     5461 aacgttatca ccctctccac tttccgtaac gctgaatttc tgcaagccta cggtgtggca
     5521 attgctgatg gcccactgaa aggtctggca gcgcgtgccg ttgtggttat tgacgaaaat
     5581 gacaatgtga ttttcagcca gctggtggat gaaatcacca ccgagccgga ttacgaagca
     5641 gctctggctg tactgaaagc ataagttaat atgcctgatg gtgttgcacc atcaggcata
     5701 ttcgcgctta ctcttcgttc ttcttctgac tcagaccata ttcccgcaac ttattggcaa
     5761 tcgcggtatg tgaaacgccg agacgttttg ccagtttgcg cgtgctggga taattgcgat
     5821 aaagctgggt taataccgag cgttcaaaac ggctggtgat ttcgtccagc gaaccttcca
     5881 tcgcatcttc gcccacggct accgttgcgg cgtcataatc cggcaacaaa atatcctgtg
     5941 gacgcagctc ataaccgtcc agttgtgtca gtgcgcgata gatagcgttc tttaactgcc
     6001 gcacatttcc cggccacgca taacgtgtaa gtacagtatt caggtcagcg gccagtttcg
     6061 gacgcggcac gccctgctcg tcggcaaagc gggcgacgaa cagctcagtt aacggcataa
     6121 tgtcctgcgg acagtcacgt agcggcggca gattgagcgt caacacgttc agacgataat
     6181 agagatcttc acggaacacg cctttttgca ccagttcgac cagattcttc tgcgtagcgc
     6241 aaatcacccg cacatcgaca tgcacctcat ggtcttcgcc aacccgacgg aaagtgccat
     6301 cattaagaaa acgcagtaat ttcgcctgca tccgtggtga catttcccct atttcatcca
     6361 acagcaccga accaccgttc gcctgctcaa agaacccttt cttcccttcc ggagcatgac
     6421 caaacagttc actctcgacc gcatcttctg gtatagacgc gcaattcagc gccaggtaag
     6481 gtttgcccgc tctggggctt gcctgatggc aggcgtaggc aaaaagatct ttacctgtac
     6541 ctgtgtcacc cgtaatcagc agcggcgcgc ttagcatcgc cagtttctgc gcctgttcga
     6601 caacgtgctt cattttcggg ctgacggcga caatttggct gaaggcgctg acgtcctggg
     6661 cggcgacatt ttgcaactgg cggcccatac gaatcgttga tcgcaacatc accaccgcac
     6721 cggtcaggac gtgttgatca ttttcatcct gaagataaac aggcgtaatc tccatcagga
     6781 aattctgccc attaataacg acatgctcgt tatgcgaatc ttgcggttcg ctttccagcc
     6841 aacgtaaaaa attaaagccg ttaatcaatt gtgcggcggt atggttgcgc aggcgatcca
     6901 atttttgccc aaaaagctga cagctcgccg ggttcgccat atccactttg cttttcatat
     6961 cgacagagag cacaggttca ggcaacgcct ccagtaacgc gctcaacgcc agatgctcac
     7021 gttcggaagg catccacggg acagtacgca catcggtaac acctgcaata cggcgtattt
     7081 cggccatcag actgctgaaa ctctcaaact ccagttcagc aaaattgagg tagattcgcc
     7141 caatgggatc aatctcaata ccgcgtaaat caatgcctct tagcacgagt agatcgagta
     7201 attcgcgggt cagaccgagt cggtcttcac aaaagacttc cagacgcatg ggaaccttca
     7261 cctgaaaaaa gaacaattaa tatgatgata tgacactatt tggatagcag gaaggtttct
     7321 gtcaacaatc attgacacaa agacggaaaa acgggcactt cggcgtaaag atatccggtc
     7381 agcacccgat attgcattat ttttcgctgg gcgttttgcc tttttgcaga gtttctttca
     7441 cctgaccgat aagctgacga cggaaatccc caaggcgagg tttgtcatcg tcaatccacg
     7501 gcagcgggcg gcaaagctcc atagctttaa tcccgagtcg taccgtcaga agtccagcac
     7561 caattccctg agctgcgcgg gtagacaaac gagcagcgag atcttgcgac atccagtcca
     7621 tccccacttc gcgcaccagt tcgctggctc cggcaaaagc gatattcagc aataccagct
     7681 taaacagacg caaacggctg taatacccca gttcaatgcc atacagcgtg gcaatgcgat
     7741 taattaaacg cagattgcgc caggcgataa acgccatatc gaccaacgcc agcgggctga
     7801 ccgcaatcat caacgttgat tccgccgccg aatggctgat ttcgcgccgc gcctgggcat
     7861 ctaaaactgg ctggaccaaa tgcgcataca ggctgaccac ttcacgatcg ttctgcgttt
     7921 catggattga ggcataccag cgttgcagcg ccggatgcga ctgatcaata cccgcctgct
     7981 gcgccagttt ttcgcaaaat gcgcgacctt tgcccgtgcc gtggctatgc aataaatcac
     8041 gcgcttcgtc gcgttcatgg gcgcgctgtc gcaagcgcca taagcgccgc cactctgtta
     8101 ccacagaacc tacgccagcg ccgataatca atgccccagc ggcacatcca cccagcgcta
     8161 cccagtcctg ggtttgccag gcattcattg tccactgtat accctgcccg acaacgcttg
     8221 cgccaaacag agccagcccg cccatcacca ttttgcgcca caggctgcgt ttcggacgta
     8281 acgctgcgtc cattaccgct tcgacttgcc cctcttcttc ctgcgcttcg tcgagcgtgg
     8341 ccggggcaaa attttgcgcc tgattttcgt caaaggtctg ctgcgccctg aattttggat
     8401 tctgttcgac ctccagcgga ccgtcgaaat caatacgtgg ttttaacggt tcggtcatcg
     8461 caatttatct cctattaaaa attccagcgc ggcatcaaga cgaatatgcg gcagcggttt
     8521 gtcgacatcc atcacctgcg ggcgaaacgc ttcaaactgg aacccttgct tgtcccagaa
     8581 cgcctgaccg ggcaaacgcg caggaacttc accggggtaa acggtgagag gcgcaccatc
     8641 gctgagccgg ttaccgcgca atgccggaat tttctcgccg ttgacgtcaa taatgccgct
     8701 ggtggtcgcc tgcaccgagg ccagccccag gcaatccata ctaatccttt caaacgccgc
     8761 attttgccag gcatcctgaa tcagttgttg cagtaatgaa accatattgg cgtgctgatc
     8821 gaccgttaca tggtccgctt tcatcgcggc aaacagcagt ttgtcgataa ccggcgagaa
     8881 taagcggcga aacagcgtgc gctgcccgta gtggaaactt tgcatcagct gcgtcagtgc
     8941 cagacgcata tcattaaatg cctgtggccc actgttgaga ggttgcaggc aatccaccag
     9001 cacaatctgg cggtcaaagc gcagaaaatg attcttatag aaccccttca ccactttctc
     9061 gcagtaataa ttaaaccgct cgcgcagcat tccggcattg gtgtgcttat cggcctgcgc
     9121 cagtttggac tcgtcccagg catcgacatc cggccacggg aagaattgca gcgcgggcgc
     9181 acctgccata tctcccggca agacaaagcg cccaggctga ataaagtgca gcccctgctg
     9241 tttacagtgg tggagataat cggtccacac ggcggcaatg tctgccagcc ggttttcgtc
     9301 ggcaggcgct agcgggtcaa gtccttcgca catcattcgc catttcgccg accattcgcc
     9361 gcgctgacca ttgagtaagc ccgtcatctg gcgcgaccag cttaagtaat cctgcgccag
     9421 catcggcagg tcgagcaacc attcgccggg ataatccaca atttccagat acagcgtgga
     9481 ggtatcctta aagtggcgca gcagcgaatc gttcgattta aaacgtaacg ccaggcgaat
     9541 ttcactgacg ccgcgcgtag gcgtcggcca ggcgggagga tcaccatata actgcgccag
     9601 tccttcatca taggtgaagc gcggaatgcc aaagtcacgc tggggaatgc gttttacgcc
     9661 cagcaagcgc tcttcacgca ccgcgcttaa cagcggcaaa cgtgctccgg catgaatatt
     9721 gagcaactga ttaaccatcg cggtgataaa cgctgttttg ccgctgcggc taagtccggt
     9781 tacagcgagg cgcagatgtc tgtcgacacc ccgattcacc agcgcattga gttcattttt
     9841 aagtcgcttc attgctgtcc tgtgctgctc tgaagaccag aaaaaaaatc attcgtaagg
     9901 atatgaatgt gggggccagg ccattgatta tcaactgccc gtgttgtcat cgacgggcga
     9961 actccataag atgtgcagca ttcacgttcg gacggagttc ctgacctatg tcccctacta
    10021 tttatgatat tgccagggtt gcaggcgtat caaaatccac cgtatcacgc gtgctgaata
    10081 agcaaactaa tatctccccg gaagcgcgcg aaaaagtgtt acgggccatt gaagaattac
    10141 agtatcagcc aaacaagctg gcccgcgcgc tgacctcttc gggttttgat gccattatgg
    10201 tgatttctac ccgttcgacc aaaactacgg cgggtaatcc gtttttctct gaagttttgc
    10261 atgccatcac cgccaaagcg gaagaagaag gtttcgacgt gatattacaa acgtcgcaca
    10321 atccggcaga agatctgcaa aaatgcgaaa gcaaaattaa acagaaaatg attaaaggca
    10381 ttattatgct cagttcgcca gcggataagt cattttttgc ccaactcgat aaatacgata
    10441 ttccggtggt ggtaattggc aaagttgaag gtcaatatgc ccatgtttat tctgtcgata
    10501 ccgataattt tggtgacagc attgcgttga ccgatgcatt aattgaaagt gggcatcaaa
    10561 atattgcctg cctgcatgct ccgcttgatg tccatgtttc ggttgatcgc gttaatggct
    10621 ataagcaaag tctgtccgcg cataatattg cagtgcgtga taaatggatt gttgacggcg
    10681 gttataccca tgaaacagca ctgcaagcag cacggcaatt attaagtcag tcaccgttgc
    10741 ctgaggcagt gtttgccact gacagcctga aattaatgag catttatcgt gcggcggcag
    10801 agaaaaatat tgctattccg cagcagttag cggtggtggg ttatagtaat gaaacgctgt
    10861 cgtttatttt aacgcctgca ccaggcggca tcgatgttcc gacgcaggag ttaggtcaac
    10921 aaagctgcga gttattattc cgcttaattt cgggaaaacc gtcaccacag aatattaccg
    10981 ttgccacgca tatgacgctg aaataaatac cagctacctt gcctgaatgc cggatgcgat
    11041 gctggcgcat cttatccggc ctacatttat agatcggata aagtactcgc gccgcatccg
    11101 gcaatttagc ccattatcag aacgagtaat ttacgccgac acctgcataa tggaatttat
    11161 cgctgtcgcc ttcgtcgtga tcctgccact caaacgcgta ttccagcgaa acggaaagtc
    11221 cgttctggaa atcataaccg taaaataaac ccacacggtt aaaatcatgg ccttcacgtt
    11281 caatatcatc ctgccagtcc cagttactct agcgatccag cccaatgcgc gtatacggcg
    11341 tcaccgagtg gttgccgagc gtcagcggca aataggcgcg aatttcttgt gtggaaaact
    11401 caccgttatt gcggctgtcg tccatattga agccgcgctc gagataatag ttcactcgca
    11461 aagcaaccgt ttcgttgaag gtatattgca gacctgtttc cgtttcgaca cgggtatcag
    11521 cgtaaccggt cgtgttcaaa tcgttggcaa atttatacat cgacaaccaa ccgttgaaac
    11581 gtaaatcatc agtcagtttc acatcccagt ctggcgcgat tttccagcgc tgcatattcg
    11641 ccgtgtcttt gcccggttca tcaacgtagt gataaccata attacggaaa ccgccggtca
    11701 ggccgaaact gaaatcatcg ctttcgagga actgataatg cacctccagc tccgggcgat
    11761 caaacctcgt tccacgttta cccgcgctat aatctaccgg cccttcctga taataggcca
    11821 gagcaattct ccacggcccg ttggcggcat taaaatagac tgaaggctcc gccagcccat
    11881 ccatatcttc gccataaccc tcgacgtttt ctatttcgta catcgcaccg atattaaagt
    11941 gccagtcgct cttttcctcg gcctgtgcgc aggccattcc cgcacacatc accagtgcgg
    12001 tacagggtaa taactttttt tgttccttgt attatcctgg gggaatgcct tccccctgta
    12061 tttagcgaat tgctatttcc gtttctgcat caaagaaatg acatttcgtc atatcaaaat
    12121 gaatagtgat attttctcct gcatgataat catttaacgc accagcacgg accactaact
    12181 cgtgccccca accgtggtgt agagcataaa ttccgcaccg gttaattctg ccacgctaat
    12241 tttggcggaa atgttatttt cctcttgcgc gtccggatga atatcttccg ggcgtattcc
    12301 catcacgatg ggcttatgca aactttcctg tgttttcaga accgctaatt tctcttcgag
    12361 aatggttaat ttaagcgttt ccgtaacgaa tttatcgcca tcgatcgtgc cgcgaataaa
    12421 attcatcgct ggtgatccaa taaatccggc aacaaacata ttcgcgggtt ggttataaac
    12481 ggttttcggc gcacctactt gctgaacaat cccgtctttc ataatcacaa tccgcgccgc
    12541 catggtcatc gcttcggtct gatcgtgggt cacgtagatc atggtggtgt tcagtttctg
    12601 atgcagcttg ctgatctctg cacgcatttg tacgcgcagc ttggcatcaa ggttagagag
    12661 cggttcatcc attaaaaaca cgcccgcttc gcgcacaatc gcccgcccga gcgccacacg
    12721 ctggcgttgc ccgccggaaa gcgcgcccgg cttgcgtttc aggtactcac gcaggccgag
    12781 aatttgcgcc gcccagttca cccgctcatc aatcacctct ttggcgattt tttgcatctt
    12841 caggccaaac gccatgttgt cgtaaaccgt catatgcgga tacaacgcgt agttctggaa
    12901 caccatcgcg atattgcgtg ctttggctgg aacgtcattc attcgtttgc cgtcgatcaa
    12961 cagatcgccg ccgctgatct cctcaagccc ggcaatcatg cgcagggtgg tcgatttacc
    13021 gcagcccgac gggccgacaa atacgatgaa ctctttatcg gcaatttcca ggttgaagtc
    13081 cttcaccaca tgcacctggt tatcgtagat tttttgaata tgttgtaacg aaagctgagc
    13141 catgttgatt cctttgctat acgttttgcc agaaggccga taaccgcgac caggtcaggg
    13201 agtccgttga gggcaacagt aattgcgccc cggttaagcc cgcaccgatc cccaccgagc
    13261 gcataccgct ggcgttaatg gcgtcaatgc ccgcctgcgc atcttcaatg ccgatacatg
    13321 cctgcggcgg cacgcccagc ccggcacagg cagcgagaaa gatttccgga tccggtttcg
    13381 agtttttaag ttgggaagca tccgcgcaga aggtgaaaaa ctcgcgcagc tccagcgccg
    13441 ctaaaatcgt cggcgcattc agggagacgg aagccagccc taccggaatc tgctgtgcac
    13501 ggagctctgc cagcaaattg cgaataccgg gcagcacagc attaaccgtc agctcgcgca
    13561 gtgagtggac gtaaagcaga tttttgcgat acgccagttg cgcccgctcc tgcgggttaa
    13621 agtcgccctc tttgcccccg tgttgcagaa tgcgccgcag agactcatcg cggctgatcc
    13681 cttttagaga ttcgttaaac tgcgcatcaa tactgatgcc aatttcagcg gcaatctgct
    13741 gccacgcctg gaaatgcaga tgcgcggtat cggtgattac accatccaga tcgaaaatta
    13801 ccccttgcag tttcattcat cctcctgatg cgtggtagcg gtcccattga agggcaaaat
    13861 aaaatcaccc aaacagaaaa cagattcttc agccacggat ataagctgac cgttcaaacg
    13921 cagtgaaacg ggcgcagaag ttcgaatcgc aatacgctgc gcgtcgagag tgacctgtaa
    13981 ttcgcagccc tgccagaaca gagggaaaga caactgttgc cactgctcaa gtaacgccgg
    14041 atttagatgc aattcaccgt cacgcacgct caccccggca aaaccctgaa tcgcccccag
    14101 ccagatagcg ccagttgcgg cagcatggat accatcatca caactatgcg gatcagcacc
    14161 aagatcgatt tcagtcccct cgcgccagaa ctgataactt tgggtcagca ggccacagcg
    14221 tgcagcaaca atgccgtgga ttgctttact taatgacgag tcgtgaatag tgcgcggttc
    14281 ataaaattgc agattggcaa gacacgatgc cgatgagaac tgctctggca gcatgtaatt
    14341 gagcatcacc acatcagctt gtttgaggat ctgcatctcg ttcacttctg cgcgtgaata
    14401 atccagcaga atggtttgct tccccgccgc cgctttgtat ttcgccagat taatcgccgg
    14461 cttagtcata aacgaatcat cctgcggcaa aacaccgtcg ggctgaattt ctggcagccg
    14521 cagttcttta aggaacattt cggcacgatg aataaacgcg tcgtcactac agccaaactg
    14581 gcgggcaata ctcagcgcct gctggacgtt gtaatacacc atatagctgg tgaaggcgtt
    14641 attattgaca tgttcggtat attcgtctgg cccaataaca tcatgaattt ccagacggtc
    14701 gttaaccctc accgcgcggc taatccagaa ctttgcagtt tccagaagta gcgccatgcc
    14761 ttcatgcgca atgaaacttt catcccccgt ggtctgccag tattgaataa ccgcccaggc
    14821 gatatcggcc accagatgat gttccgcctg cgccgaggcc actttttgcc gcagcccggt
    14881 gcgaatgtta atggcggcaa attccggcgt ctcttcttcg ccgctgcgcg cgctttccca
    14941 cggaaatagc gcgccctgcc agccgttgcg tcgcgctttc tcctgcgcgc ctggcaagtt
    15001 gtgccagcga taacgcagta aacttcgggc aaccgtcgga tcgctaaaca gatggaacgg
    15061 cagcaaaaat acttctgtat cccagaaaac gtggcctttg tagccttcgc cggttaagcc
    15121 ttttgccgca atactgctgc gctcgccgtg agccggcgtc atgatgcgca gatgataaag
    15181 cgcataatct aacgcttgct gatcgtgcgc atcgccgcca tttaccgtga tacgacgttt
    15241 ctgccaccat tgacgccagt tttctgttga tgctgcaaga tgttggtcgt aactctgttg
    15301 cgcgcacatt tcaagctggc gaagcgacac gctgccccac tcgtctaaag cagcttgcct
    15361 gtcatcccgc cagtcgatcc acaccagttt ttgcaacgtc actgtctcgc ctgcatgaag
    15421 ctgcgtactg gtatgttgca gtaaacggcg ctctttggcg gtatagcatt gctgcacatc
    15481 accgctcacc tggcagcaac aactgatggc cacatcactg cgcccatcct gggtggtgta
    15541 gatcccctgc atcaaatgct gaccaaacac ccgcacctgg gtttcgtcga gatgctggcg
    15601 accgtggttg gtttgcgtgg cgtcaatgcc tgttgaaatc agcactgacg cgtcggcgtc
    15661 cagtggcata atagtgattt ccagcgcaat gagcggcagt tggtctgccg aaacaaagcg
    15721 acggctggcg atggtgtaac ctgcgccgtt gctggtacgc cagacaacgc tacggcgtaa
    15781 ttctccactg gcaaagtcaa gttcacgctg ccaggcttcg cgggataacg agaaaacctc
    15841 accatttatg gcaatctcca tccccaagat atcaggcaga ttcaccagtt cgttgatttc
    15901 accttttccc gcccgatgat acagccccgc cagatacatc cctcgcgtct ggcgcgtgta
    15961 atcttcttca tggctggcgc gaagcccaag atagccgttc ccctgcgcca tcagcgatgc
    16021 atacttgttc agggtatgct ggctgaaatg gggttctgat aacgttactg gcctggtcat
    16081 aattccacac gcgtccctgt ttctgcggat tgatataacg ccgccaccag ttgctggatg
    16141 atgtacccct gctcggcgtc ggctatcatc acgggcttgc cctgtacgtg attgataaag
    16201 gcttccatgc tgcgcaaatg gcggttgtcg tctgccattt cccgttgcat cagcgtcatt
    16261 aattcaccgt tgttatcggt gtagatatgt gctggaaaca gcgtcgcacc ggctttatca
    16321 ccacaaaagc tgacgttcat aatcaactgt tcgcggatgt tgagtgcaaa tgacgtttcc
    16381 agccacagaa tgccgccgtt atgaaattca atggtgccaa acagcgaatc ttcgacgctg
    16441 taagttgccg gatcccactc gccaaattga ccacagctct tttgcgtgcc gatcttttga
    16501 aaactatgcg cattcacgct tttcaccgcc ggaaagccca gcacatacat cgcagcatcc
    16561 agcatatgaa tgccgatgtc gatcagcagg ccaccaccct gcagttcttt attggtaaag
    16621 acaccccagc cgggaacgcc gcagcgacgc agggcgcggg cggtggtaac gtaaatctct
    16681 cccaaaacgc cgttggtcac ctgttcacgc agctgttgcg tatcgagcgc aaacgatggt
    16741 gaaagtcgta ggccagcacc ttgcccagtt tgcgcgcggt atcgcacatt tcccacgcct
    16801 gttctgtcgt catggcgggc ggtttttcgc acatcacatg gcagcctgct tccagtgcca
    16861 tcagggtatg ttcgtaatga aaacggttag gtgagccgac gctaaccaca tcaggtttca
    16921 ccgccagcag catggcctgc ggatcgtccc acacggatgc attcccgtat ttttctgcca
    16981 gcgcctgcgc ctgggaaagg cggctgtcac agacagccac cagttccaga tcgttgcggg
    17041 tgcagtagta cgaagcatga accttatccg ccacctggcc cgcgcctatt atcgcgaccc
    17101 gcagcggaga gcttgtcatt gcacttttca caaagacctc ttagcaggta cgcaaccagg
    17161 ccaacgaatc acggtacgcc tgggcaggat cttctgcccg gatacgccct tcatacacca
    17221 cataaccctg atagttatcg gcgcgcagct gttcaaacag cgcgtggaaa tcgagggtgc
    17281 cactgcccgg ctggtaacga tggttatcgg caatatgcac atgaccgagc aagtcacggt
    17341 tgtcgtgcag cgcctgcgcc aggttatctt cttcgatgtt catgtgatag aaatcgccga
    17401 taatctgcac atgtttaaga ttgttttcga cgatgtaacg acgagcatcg gcgagggtgt
    17461 tgatcatatg atcctgatag cggtttaacg gttcgagata caccacggtt ccggtacgcg
    17521 cggcgacctg ttccagtacg cgcagggaat cactcaccag tttgcggtcg ccgtccaggc
    17581 tacgcggcga ggtcatcggc ggtaagcgga aggtaaacat gccccacgct gccggaaacc
    17641 ccccacttct gccagcgcct cgagaatgcg ttcgatctgc tttaagccat taagacgacg
    17701 ctcttcgata aagtcaccaa tccagccgtc atagccgccg caggcggtgg tcactggtaa
    17761 accggtttct ttgatcgccg ctttgacttc ttcgatgttg ttgaccagca gtttgccgtc
    17821 aatctcaaaa ccatcgaagc ccatctcttt gatataacga aatctctcgc ggatattttc
    17881 cggaaagaat gcctgattct gtgtgccgat tttcattttt tcattccttt gaattaaaac
    17941 gtaacgccca ttttgatgct ctgttccgga tgctgatcga catactgcat gtagctttcc
    18001 gggctgttgg caaaggtcac taccgggtcg attaaatctt cgcaattgag ataaccgttc
    18061 atcagcagtt cccagcaggt ttcttcaata cgcttacggc tccagcgcgg ataatccggg
    18121 ttcggttcgc tgcacgcgcg ggagaaaaca attttggcgt tattgaaatg cgcttcgcgt
    18181 ccgaggttaa aaccttcagc aaatggtttg gcaaacgcga cataggagat ggtgccgccg
    18241 taggccaggc cgcgcagcgc cgattgcagc gcgtcggcgt aaccgctggt ttcgataatc
    18301 acatcagccc cctgcttgcc ggtcagcgtt ttgatctctt tgcctacgtc agtgccaatg
    18361 gggttaaggc agaaatcagc accgtggcga cgggcaatat cacagcgatg ggcgatagga
    18421 tcgacgccaa ttaccaccga tgcgccagcg cgtttcgcca gttggatggc aatttgaccg
    18481 atcgcgccaa gccctaccac cacgacaaaa tcccctacgc gtacgttggc atcgcgcacg
    18541 ccgctcatgg caaactgcgc cgggtcgtaa cagacggcgt ttttccagga gctgccttgc
    18601 ggcattttgc gcagcttgta gttattcact gcgttaatga tgaccgtctc ggagagcggg
    18661 ccgtagccgc atacgctgtc gcccaccgcg tagtctgtaa cgtcgctgcc gcactcgata
    18721 atgtcgccaa ccaccatgtt gccaagctgg aatttgccaa actcaatgcc gcgcggcgca
    18781 tctgccggac gcggggtgaa catctgccac tcgccgttaa agtcttcatc gataaacggg
    18841 ctggcggcgc ggaagtcgac cacttccgtt ccgtgtttcg gtgcgccgaa acgcacgcgg
    18901 atcttcactt cattggctaa aatcgcccga tcttcatact caaccagcgc agcaacacgc
    18961 ggtgctgtgg ctactaactt tttcatgtaa aactccttgt aaagaaactg gccgctttag
    19021 cccttcacgc caccggcggt caaaccactc ttaataaaac gttccgaaag cgcatacata
    19081 atcaccaccg gcaatgcggt caccagcgag gccgccatca tccgtcccca gatgtaatca
    19141 ggcgtactga acagcgcgtt caggcccact ggtaaggtga aattgctggc gctggagagg
    19201 aaaatcgacg caaacagata gtcgttccac gccaccataa agcagtagac gaacaccgat
    19261 atcagcccgg acatcgccag gggcacggtg atacggaaga tgatttgcag acggttgagg
    19321 ccatccatca tcgccgcttc ttcgatctca tcagggatgg tgtcgaagta acttttcagc
    19381 ataaacacgg cggtgggcag ggtttgcgtc accatggtaa tgatcagcgc catctcggtg
    19441 tcgtaaatac caagcgcggt gatgattttg aacagaggca ccaccagcaa aatgccagag
    19501 aacatataca ccgtgtaaaa gctggcgttg atggtcatcc ggcctttaaa gcgcaggcgg
    19561 gaaagcgcgt aagctccgag aatgccgaga aataccgcca ccacggagga aaccaccgac
    19621 accaccatgc tgttacggaa gtagtcgaca aacgggaaaa tcatcgggtt aaaaatgtcg
    19681 acgtaatgct ccagcgtcca ctgttgcggc agcagcgtag gatgcagcga tatcgcctct
    19741 ttcgcgcttt tgaacgaggt catcagcatc acaaaaaacg gaaacagcgt gatgatgaga
    19801 aacagcgcca gcccgcagta aaaaccgata cgactgagcg tgcgtttatt tgttgccatt
    19861 gaggttcacc cgtttcctgg tcagcagaat gacagcgaaa ataatgatga agagcaccac
    19921 cgagatcgcc gccgctttcc ctaagtcatt aaaggcaaag gcggttttgt agagatagac
    19981 accaagaata tcgaccttgg ttgtcagcag ataaacatca gcgaacatgt agaacatcca
    20041 gatggtgcgc agggtcacca ccgtcgccag gaccggcata attgcgggca gcgtgacgat
    20101 gcgaaaccgc tgccaggcat tagcgccatc catctctgcc gcttcgtaca gcgatttgtc
    20161 gatggtctgc aaaatcgcca gaaacgagat aaaggcatac gggaagtagc gccagatggc
    20221 gaacagcacc accagcacaa agctactgcc cggattatca aaccacagcg gtgcctgctc
    20281 atagagatgc agaagatcga cgccgaggta gttcacaatg ccgtagccgt tgttgaacat
    20341 gtatttccag gcaaacacca gcgaaatgga cggcgttacg taggagagga tcaccagcga
    20401 acgcgcggtt ttgcgcaggc ggaactcgcg gttgaaaaac atcgccacgg ccagcccgag
    20461 cacggtgctg cccgccacca ccagcgccgt ataccagacc gtaatccaca gcgagtgcca
    20521 gaagccggga tcggagagaa tgcgcacata gttactcacc ccaacaaacg ttgactcgat
    20581 gttgggattg agcggcagac gtaaaaaact gatttcgata ttcgacacca tcggccacgc
    20641 caccagaccg cccagcagta ataagctggg cgcgagaagc agcagcgcaa acggcatatc
    20701 ggaacgacct gaaaaaagcc tgttcattgc ggattcctgt cctgggttta gtgctgttcg
    20761 atcagttcat ccagcttttt ctggctcgct tgcagcgtgc cagggagatc ggctttaccc
    20821 acggtgacgt tatgcaccat ggagctcacc acaccagaac ccgtcacatc gcccatgcgg
    20881 gtaaagtttt tatcccctac tgcgccaaag acctgaatat ttggcagttc accgattagc
    20941 tgattcggta gttcacccag cgccttaata acgtcgttgt ctttccaggt ggcggtagtg
    21001 acaaccgctt tattcaccgg cagcgcagca cctggcgaca tcatcaccca gtcggcaatg
    21061 ttgtccgcct gctccataaa ggtgacaaat ttttctgctg cttcggtctt ttgtccggcg
    21121 gtaatggtca gcgaggtcaa catgccgtag accgcagagt ttttctcggt tggcaccacg
    21181 aaaccgacgt ttttcgggtc gccttcttta atcacagccg gaaggatata ggtggagtaa
    21241 atcgccatcg gcgcggtgcc gttcataaag gcgtctttca cttccatgat gtcgttagaa
    21301 cccggcatgg tgttggcagc aaggtcgcgg taataggtca gcgcctgcat catctctggt
    21361 gtatcaaggg tgattttgcc ttcggcgtta aagacgttgg cctagttgga taacgcaaac
    21421 tgggagaagg attgttccgt cagcacgctt tctgccgtag gcagcgcaat accgtatttt
    21481 ttattcgccg ggtcattcag tttctgtgca acgtccagca gctgttgcca gtttttcggc
    21541 tcctccagcc ccgcttttgc cagcacatct ttgcgatacc agatgtcgcc aatccaggcg
    21601 ctgacaggaa caccggtcca tgcgctacca tcttcggtac gcacaatacg cagtacgcca
    21661 tcgtaaaacg cgccttcacc aacgttgctg atgaccgtgg caaccgcttt gcgatcgata
    21721 agctgctctt tatccataac tttggcgtag tcatggctgg tttcgatcac ttccggcagc
    21781 gagccgctac gtgaaagggt gatgactttg gtgttatgag cgtcttcttc cacgggcact
    21841 tgtttaacgg tgatacctgg gttttctttt tcaaaacggg caatcaattt gctgataact
    21901 gcctgacgct cctgctccac cgaagaatgc ataaattcga tggatacatg ggttttattt
    21961 tcttctttac agcctgaaat cagggcgcag gaaaccagtg ctgataacag cacgattttt
    22021 gatttaatca tgagatgttc ctttttattt aatccacata acctgccagg ggcgcagcgt
    22081 tatttcatta cccaatatat caacttcact aatcagatcc cgaccatgca gatcagtaat
    22141 atttacatgc tgaatatttt tactgacatt aaacaatcca gtcagacaat taccatcagc
    22201 gttacttctt tgaatacgca ttacggatga attaaccgta tcgatggtaa aattattatc
    22261 cggatgaaac tcgttatggc tgcgacgaag tgtaattaaa cgcgacaact catgatatac
    22321 cgcatgcctt aatgtagctt catcgttcag ttctcgtgtt atctcttcac tgtgatattt
    22381 tttacggtta atcgcacggt tatatccgag tttttcgaca cctgcgtaat cattacgcga
    22441 gccaagaata ctttgaatat atatcgcggg tacaccgggg aaacttaaca aaatcgcatg
    22501 ggcaaggata aacctggtgc aacgttcttc atcgctactc tcgcggcggc ttaacgcatt
    22561 catataggtg acattcatct catagggact gcgcgtgccg tcgggattat ttttccagtt
    22621 tact
//
LOCUS       DAEEUE010000060        22512 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.060, whole genome shotgun sequence.
ACCESSION   DAEEUE010000060 DAEEUE010000000
VERSION     DAEEUE010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22512
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.060"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..603)
                     /gene="capU"
                     /locus_tag="KLK84_002518"
     CDS             complement(<1..603)
                     /gene="capU"
                     /locus_tag="KLK84_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HBE2009801.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKFGIDITFALFRNSLHIPTVWRLLGIVHSFQPDAIVCHSGHDSNIVGLVRFFTW
                     KHPFRIIRQKTYLTRKTKFFSINHFCDEVIVPGTNMKTHLEQEGCRTRVTVVPPGFDF
                     QELYVDSRNSLPPSVLSWLASRRGCPVIAQVGMLRPEKGHE"
     gene            complement(606..1448)
                     /locus_tag="KLK84_002519"
     CDS             complement(606..1448)
                     /locus_tag="KLK84_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBE2009802.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     SFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELG
                     FHYLYTTERRMNAPAKGTTRIGRISTKERENCVWLKRRLFYYTTPFFSSLLALHKGPR
                     LPDD"
     gene            1634..2351
                     /locus_tag="KLK84_002520"
                     /pseudo
     CDS             1634..2351
                     /locus_tag="KLK84_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3024..4532
                     /gene="ltrA"
                     /locus_tag="KLK84_002521"
     CDS             3024..4532
                     /gene="ltrA"
                     /locus_tag="KLK84_002521"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBE2009803.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPEPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
     gene            4613..5100
                     /locus_tag="KLK84_002522"
                     /pseudo
     CDS             4613..5100
                     /locus_tag="KLK84_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            5183..5440
                     /locus_tag="KLK84_002523"
     CDS             5183..5440
                     /locus_tag="KLK84_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009804.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(5998..6765)
                     /gene="fecE"
                     /locus_tag="KLK84_002524"
     CDS             complement(5998..6765)
                     /gene="fecE"
                     /locus_tag="KLK84_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HBE2009805.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(6766..7722)
                     /gene="fecD"
                     /locus_tag="KLK84_002525"
     CDS             complement(6766..7722)
                     /gene="fecD"
                     /locus_tag="KLK84_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HBE2009806.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(7719..8717)
                     /gene="fecC"
                     /locus_tag="KLK84_002526"
     CDS             complement(7719..8717)
                     /gene="fecC"
                     /locus_tag="KLK84_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HBE2009807.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(8714..9616)
                     /gene="fecB"
                     /locus_tag="KLK84_002527"
     CDS             complement(8714..9616)
                     /gene="fecB"
                     /locus_tag="KLK84_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HBE2009808.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(9661..11985)
                     /gene="fecA"
                     /locus_tag="KLK84_002528"
     CDS             complement(9661..11985)
                     /gene="fecA"
                     /locus_tag="KLK84_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HBE2009809.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPIS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(12072..13025)
                     /gene="fecR"
                     /locus_tag="KLK84_002529"
     CDS             complement(12072..13025)
                     /gene="fecR"
                     /locus_tag="KLK84_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HBE2009810.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(13022..13543)
                     /gene="fecI"
                     /locus_tag="KLK84_002530"
     CDS             complement(13022..13543)
                     /gene="fecI"
                     /locus_tag="KLK84_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HBE2009811.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            13841..14023
                     /locus_tag="KLK84_002531"
                     /pseudo
     CDS             13841..14023
                     /locus_tag="KLK84_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            14017..14121
                     /locus_tag="KLK84_002532"
                     /pseudo
     CDS             14017..14121
                     /locus_tag="KLK84_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            14461..14718
                     /locus_tag="KLK84_002533"
     CDS             14461..14718
                     /locus_tag="KLK84_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HBE2009812.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERD
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
     gene            complement(15038..15169)
                     /locus_tag="KLK84_002534"
                     /pseudo
     CDS             complement(15038..15169)
                     /locus_tag="KLK84_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            15451..16809
                     /locus_tag="KLK84_002535"
     CDS             15451..16809
                     /locus_tag="KLK84_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HBE2009813.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(17048..18433)
                     /locus_tag="KLK84_002536"
     CDS             complement(17048..18433)
                     /locus_tag="KLK84_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBE2009814.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DTIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKSIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            complement(18483..18830)
                     /gene="tnpB"
                     /locus_tag="KLK84_002537"
     CDS             complement(18483..18830)
                     /gene="tnpB"
                     /locus_tag="KLK84_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBE2009815.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(18827..19207)
                     /locus_tag="KLK84_002538"
     CDS             complement(18827..19207)
                     /locus_tag="KLK84_002538"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HBE2009816.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            complement(19562..19996)
                     /locus_tag="KLK84_002539"
     CDS             complement(19562..19996)
                     /locus_tag="KLK84_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HBE2009817.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            complement(19984..20385)
                     /locus_tag="KLK84_002540"
     CDS             complement(19984..20385)
                     /locus_tag="KLK84_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009818.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     ETELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(20645..21214)
                     /locus_tag="KLK84_002541"
     CDS             complement(20645..21214)
                     /locus_tag="KLK84_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HBE2009819.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            complement(21959..22102)
                     /locus_tag="KLK84_002542"
                     /pseudo
     CDS             complement(21959..22102)
                     /locus_tag="KLK84_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
BASE COUNT         5422 a         5889 c         5977 g         5224 t
ORIGIN      
        1 ttcgtgccct ttttccgggc gcaacattcc tacctgagca attacggggc atcctcttcg
       61 ggacgccagc caggaaagaa cactcggagg caaactgttt cgggaatcaa cgtataattc
      121 ctggaaatca aagccaggag gcacaacagt aacccgagtt cgacatcctt cctgctccag
      181 atgtgtcttc atatttgttc cgggaacaat cacctcatcg caaaaatgat ttattgaaaa
      241 aaattttgtt tttcgtgtca aatatgtctt ttgcctgata attctgaatg gatgtttcca
      301 ggtaaaaaac cgtaccaaac caacaatatt actatcatgt ccactgtgac aaacgattgc
      361 atcgggctga aaactatgaa ctattccgag taatctccat acagtaggaa tgtgaagact
      421 gtttcgaaat aacgcgaatg tgatatcaat tccaaactta ctggcttcaa aagcaatttt
      481 gctgttctcc ctgcagacaa gcagaactga atgccccatt ttcttcaggg ccttcatttg
      541 agcaacagcc tgtaattcct gaccacctat attcggtgat gattccgtaa atagtatatt
      601 cattattagt catccggtaa tcttggcccc ttatgaaggg ccagcaacga tgaaaaaaac
      661 ggtgttgtat aataaaaaag tcgtcttttc agccatacgc aattttcccg ttccttggtg
      721 cttattcttc ctatccgtgt agttccttta gcaggagcat tcattcttct ttctgttgta
      781 tataaataat ggaatccaag ttcttcagcg atctgaatat aatccttgtt ataataacct
      841 tccggccaac acaaatgttt actgcatttt cctgtcattt tttttaaata ttctctcccg
      901 ctcaataagt cctgccgaag atgtttacac tgttcttctc gtgaagtgaa ctttttatcc
      961 caacgtgtat gagtatgtgt atgaacatga aattcgacca gaccagattg caacatttcg
     1021 ttcacttcag accagcgaag cataacatta tcagcattcc cggttgctat ttgatgttcg
     1081 caatcgcgat gagaatattc ctttccagga gagtgacgaa ccggaccatt tccaataaaa
     1141 ctggtaatta aaaaaatatg tgccttgaga ttaaactcgt ttaataaagg atatacctga
     1201 aaccagttgt caaggtatcc atcatcaaat gtcaacataa cgcttttacg gggaagtttt
     1261 ccgccccgat aaaaaaactc cagttcatct gatgacagtg ttttccagtt gttttcagcc
     1321 aaccatttca tttgtttccg gaatgtctcg ggagaaagtg taaccagtcc tgggcaatgg
     1381 ctgacatgat gatacattaa taccggaaca tgatttgctt taaacaaaat gccccctccg
     1441 tttaaaattt aaagtttctg gcacccagta agtagagata aaaggaaagc ctaataaagg
     1501 tggtgaactt tatatactac taaatcaaac atcaacaatt tataaacaat aatatattat
     1561 catatctttt aaaaacggct cactaagaac cttgaggtgg cacactaaat ttagctacct
     1621 gaacagaggt gatatgctca cctcagaaca acacaggtgc cataatgaaa aaagaaattt
     1681 cagcgcagag tttaaacgcg aatccgctca actggtcgtt gaccagaaat acaccgtggc
     1741 agatgcagcc agcgctatgg atgtcggcct ttccacaatg acgcgatggg tgaaacaatt
     1801 acgtgatgag cggcagggaa aaacaccaaa agcctccccc attaccccgg aacaaattga
     1861 aatccgtgag ctcaggaaaa agctacaacg tattgaaatg gaaaatgaaa tattaaaaag
     1921 gctaccgcgc tcttgatgtc agactccctg aacagttttc gataatcggg aaactcaggg
     1981 cgcgttatcc tgtggccact ctctgccatg tgttcggggt ccatcgcagc agctacaaat
     2041 actggaaaaa ccgtcctgaa aaaccagacg gcagacgggc tgtattacgc agccaggtac
     2101 ttgaactgca tggcatcagc cacggctctg ccggagcaag aagcatcgcc acaatggcaa
     2161 cccagagagg ctaccagatg gggcgctggc ttgctggcag actcatgaaa gagctggggc
     2221 tggtcagttg ccagcagccg actcaccggt ataagcgtgg cggtcatgag cacgttgcta
     2281 tcccgaatca tcttgagcga cagttcgccg taacggaacc aaatcaggtg tggtgcggtg
     2341 atgtgaccta tagtgtgccc ggagttcagg gcgggcatgg atgcttaaat gaaccgcgag
     2401 tctgtctgga atattgaacc ggtaactcac gatgagaaac ccaacaatcc caccgggtgt
     2461 gacggtggag aacctgagcg gcagtgacct gcggcatgcc cgcagggtga tgtaacccgc
     2521 tgacaacggg gattgaggcg agatcactaa gccgagatga tcctcaaggt taagtactga
     2581 aaggctgaag aacatgaacc cgttaatccg cctctgtggg ttgaaaacgt caccacggcc
     2641 tacgtgatct gacaggccgt gcaggaggaa ctggcagtga tacgtaagca ctgccggtcg
     2701 aaggtgtttt gacatgtatg cgaaacaccg gggcagcagc gtctatcacg cttgcgttgc
     2761 tgacttctgc caacttgcgg caagcaagga taaagagtgc gacgggcagc ctcctcagta
     2821 tgcctgagtc caggcaggta aaccggggaa ggtcagcgac ggatgttaag ggggcatggc
     2881 tccgatgacg cgctgactgg cggagcttcc gtagtagtcc gcgatgggga aagcccatta
     2941 catggcgaag ggaagcagtt tgaatgtgtt tgcgacgtga attaactggc ctaacgaggt
     3001 gaagaccttt gataatcagc gaaatgcaac gcaagcttgc cacatgggca gccaccgatc
     3061 cgtccctacg gattcaacgg ctgctgcgtc tgataacaca accagaatgg ctggctgaag
     3121 cggcgcggat cacgctttca tcaaaggggg cgcatacccc cggcgttgat ggcgtgaaca
     3181 aaacaatgct acaggccaga ctggctgttg agctgcaaat cctcagggat gaattactct
     3241 caggccacta ccagcccttg cccgccagac gggtttacat ccctaaaagc aacggcaaac
     3301 tgcgaccact gggtatcccc gcgttgcggg atcgtattgt tcagcgggcc atgctgatgg
     3361 cgatggagcc gatatgggag agtgattttc atacgctctc atatggcttc cggcctgagc
     3421 gcagtgtcca ccacgcgatc cgcacggtga aattacagct cacagactgc ggtgaaaccc
     3481 ggggacgctg ggtgattgaa ggcgacctgt ccagttactt cgacaccgta catcatcgac
     3541 tgctgatgaa ggctgtacgc cgcaggatca gtgacgcacg tttcatgact ctgctgtgga
     3601 aaaccatcaa ggcgggacat atcgatgtcg gtctctttcg ggcggccagt gaaggtgtgc
     3661 cacagggcgg tgttatatcg ccgctattat cgaacatcat gctgaatgag ttcgatcaat
     3721 acctgcatga gcgctacctg agcgggaaag ccagaaaaga tcggtggtac tggaataaca
     3781 gtatccaacg gggccgaagt acggcggtca gagaaaactg gcagtggaaa cccgcggtgg
     3841 cgtactgccg ctatgccgat gattttgtcc tcatcgtcaa aggcaccaaa gcacaggcgg
     3901 aagccatcag ggaggagtgt cggggtgtgc tcgaaggcag tctgaaactc aggctgaaca
     3961 tggataagac taaaatcacc catgttaatg acggctttat ctttctgggg cacaggatca
     4021 ttcgcaaacg cagtcgttat ggcgagatgc gagtggtctc aacgatcccg caggagaaag
     4081 ccagaaactt cgccgcatcg ctgacagcac tgttatcagg caactacagt gaaagcaaag
     4141 tcgatatggc tgaacaactc aaccgaaaac tgaaaggctg ggccatgttc tatcagttcg
     4201 ttgattttaa ggccaaagtc ttcagttata tcgaccgtgt cgtgttctgg aagctggctc
     4261 actggctggc ccgcaaatac cgtacaggta tcgcttccct gatgaggtgg tggtgtaaat
     4321 caccggaacc gggtcagagc aaaacgtggg ttttatttgg taaaaccaat cacggcaagc
     4381 tcagcggcga aatactgtac cggttggtgg ggcaaggcaa gaagctgttc cgctggcggc
     4441 tacccgaagg taatccctat ctgaggacgg agaccagaaa cacgtataca tcgcgcttta
     4501 cagaagtggc aatggcgttc gccagcattt aaatggagag ccggatgcgc tgaaaggtgc
     4561 acgtccggtt cggggaggag aggcagggaa atagtccgac tacgccctgc ctcttactct
     4621 actctggacg ggtaagcgct gggcgtacct cgccgttgtt ctcgacctgt tcgcaagaaa
     4681 accagtgggc tgggccatgt cgttctcgcc ggacagcagg cttaccatga aagcactgga
     4741 aatggcatgg gaaacccgtg gtaagcccgt cggggtgatg ttccaagcga tcaaggcagt
     4801 cattatacga gcaggcagtt ccggcagtta ctgtggcgat accagatcag acagagtatg
     4861 agccggcgtg gaaactgctg ggataacagc ccaatggagc gcttcttcag gagtctgaag
     4921 aactaatggg tgccagcgac gggctatgta agcttcagcg atgcagctca cgcaataacg
     4981 gactatatcg ttggatatta cagcgcacta agaccgcacg aatataatgg tgggttacca
     5041 ccaaacgaat cagaaaaccg atactggaaa aactctaacg cggaggccag ttttagttga
     5101 ccacaacaga ctacctgaag ggagccgcgg tcgcctagca gttgcagtag caggagatca
     5161 tccagccgca gtacaggtca cgatgactct ggttaatgat accggctttg accccgtatt
     5221 ttccggctct atcgctgaat catggcgtca gcagccgtgc acaccatcct attgttgtga
     5281 ctgggaggct gccaccatgc ttcgcgcttt ccctctggcg aaaaagggag aaggacgggc
     5341 ccgtctgcct tcactttatg ccagcttcgg taagctgggt gagacaccga ctcatgaaga
     5401 tatcattgat aacaatcgat ccatcaactg gcctgtataa cgtggctgcc ggtgattaag
     5461 aaagctgcac ctacctaagt agtagcaaac gcacactttt tagaaaaatc gatggtcaga
     5521 aactggatta gcaattccgt tccagggttg cttttgattt acgttggcgt ctgatcattg
     5581 atttatcctc aaaagcccaa cctcattggt aatgaaccag ctccgtgaat gtccgctctg
     5641 gcacagagcg aaattttttg atctcccccc ctgaaatcta aacttagtca tgtcacgttt
     5701 ttgggtttct aaaattttaa cttcgcgttt ttcgttgccg taagggttat acagaaatgt
     5761 ccgttaagca gagttcaaaa ttgattgccg tgatcacgac tggtttgaaa gccgcgccca
     5821 agcctgtaca gctctggttt gcgttgatta tgaacctgtc agcctaaagc aagcggatgg
     5881 acgatgagta ttggtaatct ttcagagtcc ggaaaagttc agccccagtc tgaacaggct
     5941 tgctggcgcc agtccagttt cattcagtcg tggtttggtt cttacggcct gtgcaatcta
     6001 cctcattagg cacatcggcc tgccagatac cggctcgggg tgtatttccg cttccacgct
     6061 gaatactgtt ctcagcaatc ctggggtcat cacctcttct ggtgtgcctt gcgccataac
     6121 atgtccgttt gccattacca ccagttgatc gcagtaccgg ctagcctgat taaggtcgtg
     6181 cagcacagcg accaccgttt tcccctgagt ccggagttcg cccatcaacc gcatcaggtc
     6241 cacctggtga ttgatatcaa gataggtggt tggctcatca agtaatacaa cgggcgtatt
     6301 ctgggccagg accatcgcca gaaatgcgcg ctggcgctga ccgccggaaa gctcggttaa
     6361 ccgacgaacg gcaagatgat tgatccgggt ctggttcatg gcgacattaa ctcgtgcatt
     6421 gtcttcagcg gagagacgcc cccagagtga cagccaggga ttacgaccat acgaaaccag
     6481 ctcctggact gtgatcccct ctggcgttaa atggtgctga ggcagcagcg aaagcctgcg
     6541 ggccaactgg cgtgatgaga gcatatttat gggattatcg ccgagaaata cggtgccaga
     6601 ctgcggcatt aaaagccgcg aaaaacagtt taacagcgtc gatttcccgc aaccgttagg
     6661 gccgatcagg gcggtgatct tccccgttgg cagtgagagt gaaacgtcgt taagtacctt
     6721 gtctgtcccg taactgaccg tcagattttc agttcgtaaa gtcatttatc gcattctcac
     6781 aagcaaccag acaaaccacg gcgcaccgat aatggcggtc agcacgccaa ccgggagctc
     6841 cagtggggga tgaataattc tcgccagcag atcggcaacc accaacagca acgcacctgt
     6901 cagggctgaa acaggcagca gtctgcggtg acgtccaccg gtgatgctac gcatcatatg
     6961 cggcaccacg agaccaataa agctaatcgg gccgcaggcg gccacgccgg tagatgtcat
     7021 ggcgacagct agtaacaaag cccagaatcg ggtatggggc accgacacac cgagcgtggt
     7081 ggcgcgcgca tcgccgagtg caaggaggtc gagatcgcgg caaaaactca ggctcagcgg
     7141 cagaaataaa atcatcagcg ggatggcaat cttcacaaag ctccagtcac ggccccataa
     7201 gctgccggtc agccacagca gggcgttgtt cacatcctgc gggcgcgaga gcatcagata
     7261 atccgtcagg ctggcccagc atgcagaaag cgccacgccg gtgagcgcca gcttcatcgg
     7321 ctggtgggtc tttgccagca tcttcagtaa tatcaacccc gccatgccgc ccgcaaaggc
     7381 cagcagcggc agcaccatca cgggcagtga cggcataaga agtagagccc ccacagaggc
     7441 caggctggcg gcatggttaa cgccgagaat atccggtgat gccagagggt tgcgcacaat
     7501 cccctgtatc agcacgcccg ccacggcgag ggctgcaccg acaaacagtg ccagcagcaa
     7561 gcgcggcagt cggtactcca tcaatacata ataatgctcg cgtccggcct gccagtcggt
     7621 cagcagcgcg cgccacggca cggggatcac tcccatatgg agtgataaca gcgcacagcc
     7681 cgccagggca agggtgatga aaataaccag cgcaattttc atcctcgcct cctcacaagc
     7741 cagacaaagc aagggctgcc aatcagcgcc agcactgcgc ctgcgggcag atctccgggg
     7801 aaggccagcg cgcgtgcgag tacatctgcc agcagcatca gcgtggcccc cagcagcatg
     7861 ctcactggca gtacgttgcg ctgatcgaag cctgcccaga agcgcgccag atgtggcacc
     7921 agcagaccga taaacgccac cggacctgcc acgctgacgc acgcaccaac cagaagcagc
     7981 actaacatat tgatgaccaa acgtagcctc gtcaggttca ctcccagcgt atgggcggtg
     8041 ctgtcgctga ggttgagcag gttcagttga ttcgccagca gtaacacgac agggactgca
     8101 gtgaccacca ccggcaagag ctgccagaca tcctgccagc gggcgtggga cactccgcct
     8161 gccagccagt aaaagatgcc gtaagcatga tcttcggcca gcagcagggt gatgcgggta
     8221 aggcccatac aaaaggccga cagcgcgata cccgcgagga tcagtttgtt tctgtcatgg
     8281 gtatgacgaa atccgcctcc tgcggtcatg accagcagcc agctcacgcc gcccccgcat
     8341 gccgcgatga acgacagaga atagcctgca atcggcgtcg gactcagcgc gctggtaagc
     8401 gccatagcca gcgccgcgcc gctgttaatg ccgagcagtg aaggagaggc cattgggttg
     8461 tgggtcaggg tttgcagcag cgtgcccgcg agcgccaggc ttgcgccgat cagaacggcg
     8521 accaggcttc gtggcaaacg aaggttttgc accagcgctt ctggtagcgt tggcgtgtgt
     8581 ccaggcagca gggcgcgggt tgcatctgct ccggaaacag gaatggccga gtagcaaaac
     8641 agactcagcc agaaaataat aataagtgct gcaacgggaa gcccccacag cagcatcggg
     8701 tgttttatcg cggtcatttc acaacggtaa gcggctgatg gtggaagatt tttaccgtgt
     8761 cagcggcaat acgctctgca gcaaaaatac cgcgcatccg cgcccaggtg ttactgtcga
     8821 ccgaagcaac ctgctgcttc tgcgcggcgg ttaacatctg ccagagcgga tcttgttgcc
     8881 agcgtttaac aatgctctct tcgcgatagt gggcaaccag cagccaggca ggattgaccg
     8941 ccagcagttg ctccaggccg atggacggca tggacgaacc cgccatcgca gcgggaacgt
     9001 tcagccccag agaggccagc acgctgccgg tccaggtctc ctgagtatgc aggttgaatt
     9061 gctgttcgcg tgatgtgcca aaggccacgc gtgtcccttt gggaagctgg ctggcccact
     9121 gcgccatcct ctctttatgt tgttccagac gtgcctgcat ctctcgcttt ttacccacca
     9181 tttcgccgat gatagccgca gattgcaaat tttcagcgta ggtttcgttg cgggacttaa
     9241 gcagcagtac cggcgcgatt tgctgcaagg cgatgtaaac ccccgcatgg cgactgctgt
     9301 cggcaatgat caggtctggt ttcagagcgg caatggcttc caggctcggc tgcgcgcgcg
     9361 ttccgacgga ctgccacggt ttcaggtgcg cacgcacttc gggcaggatg cgttttgcat
     9421 cgttatcgtc ggcaataccg atcgggctga cgtccacggc ggccagcgca tcggcgaacg
     9481 agagttccag caccacaatc cgttgtggcg ttttttcgag tgtaaacgtg ccgtgttcgt
     9541 cctgaaccat ggcggcaaag gcgtggctga tcaccagcag caggcctgca aaaagaaaac
     9601 ggataaatgc caacataatc acattccagc taaaagcccg gcaagccggg cgttaacaca
     9661 tcagaacttc aacgacccct gcatatacag cgtgcgcggc tggcctgcat agatgccttt
     9721 gttgttgtcg tcataagagc ggatgaagta gtcctggtcg aagatgtttt tcacaccgaa
     9781 tgccaggttc agatctgcca tctgcgggcc aaagtcatac gccacgcgtg caccccagag
     9841 catgaagccg ggaatgcggc cggtactgcc gtcggcgctc tctttcaccg tattggcgtt
     9901 atccgcaaac tggctggact ggaaatcgct gttcagattg aacgtccagt ttcctggctt
     9961 gtagtccacg cccagcgtgc ctttatgttt cggggagaat ggtaccagat tgccgtaggt
    10021 gtcgcctttc tcgcggattt ccgcgttcac atacgcatag ctggcgtaga tggaaacgtt
    10081 atcaagcgtt ggcgttagcg tacccagatc gtaacgtgcc tgcgtttcca gcccggtatg
    10141 gcgcgttttg ccacgtgcag tgacggtgtc gttggtctgg ttggagtcgt actgattgtt
    10201 aaagttaatc aggaacagcc ccatttccgc cgtcagcgcg ccgtcgtcgt agcgggtacc
    10261 gagttcccag gttcgcgctt tttccggttc cacattgccg ctttgcacag ccttgccaat
    10321 ctggctgtac tgtacggtgc cgaacgagcc ttcagtgttt gcataaagat tccagctgtc
    10381 agtcaggtga tagagcacgt tcaacgccgg aagcggtgcg ttatagctca cttcttcgtg
    10441 cgtgcctgtg atggcgttgt tctggtatga ctcgatatgt tcgaaacgca tacccggcgt
    10501 gatggtccag ttgccgatgt cgattttgtc atccagatac caggcgtgcg cctcggtgcc
    10561 ggaacgcgta tcgcggtcgt aagggcttga gccggacggc aactgcccgc tgctggtggc
    10621 ggtgtagtaa cgcatttcat gcgttgattc attcacatag cgatagccca cgcccacttc
    10681 gtgcgcggaa gggccgatca taaagatctg gctgtagcgt ggctcaatac cgcgcaccca
    10741 gtagttacgc ggcgagaggg tgatgcgttt gccttgctcc aggtagccgc tgcgcagggt
    10801 ttgggtgtag aacccctgaa tgttgaattt atgctggctg tctggctgga actggtagcc
    10861 caggctcgcc agcttgcgac gaccccagaa gcggtcatac gggcgggtgg attgccagcg
    10921 atcggcgtcg taatccgcgc gagacaggcc accgggcatg tcggcttcac cgtcgtaata
    10981 ttgcagcagg ctgttgaagg tgtgcacctc atccggcgca tatttgcttt tcagcatcag
    11041 gtcgtcgatg cgggtggcgc tgtgctcgcg ccagtcactg ccgcgcgtgc cggagtagag
    11101 cagcgcggtg ccaaaaccgt tgtccgctgt gccgcccacc atcaggttgt gcgtctcttt
    11161 cgggttgttt tgtgaagagg ttgggctgag ctgaccttcc acgcccgcct cgataccaaa
    11221 gtcctgcgga atggcacggg taacaaagtt caccacgccg cccacgctct gcggtccgta
    11281 acgcaccgca ccaccaccgc gtaccacgtc aatggcatcc atgttgccga gcgaaatggg
    11341 agccagtgaa agctgcggct gaccgtaagg ggcgaagggg acggggatgc cgtccatcag
    11401 gacggtcgag cggctggcga ggcgcgggtt caggccccgg atgccaaagt tcatcgccag
    11461 gtcgtggctg ccggtgccgt tgttttccgg cgcgctgacg ccagggatgc ggttaagtac
    11521 ctcacgcatg gtggttgcgc cggttttggc gaaatcctca cggcggatca cgtcacgcgc
    11581 gccagcatgt tcaaatacgt cgttttcacg cgcatcaccc agccagtcgc cgaccacggt
    11641 cagggcatct tcttttggtg cgggcgcggg ctccagcgtc cagctgttat ttcccagcgg
    11701 ttttacctgc agtccgctgc cgtccagcag ttgttgcagg ccgctctcga cgtcgtaatc
    11761 gccgtgcagg ccgttgctct gcttgccgcg cgtcaggctg gcgtcaaccg agagggtaaa
    11821 tccgctgtgt gcggcatact gattgagcgc tttatcgagc gatcccggtg cgatattaac
    11881 ctgtgcagca aaagcggaaa acgagagacc ggccagcggc agcaggctca ggcgaatggc
    11941 gttaaccaaa ggtgttgttt tacgaaaaac gcgtaacggc gtcatacctt ccccatcatc
    12001 atttttgttg tgttcagcta tgagtcgaac gagaagggta aaaaggacaa tcgaaataag
    12061 aattattttc cttacagtgg tgaaatgttt atccagtacc gcgtaataga ctgaatttta
    12121 acgggaagcg tttgcgcgat aacgttcagg atcgcatcgg tatttttcag cgggaacgtc
    12181 ccgctcaggc gcagcccggc aacggcggga tcgcagcgca gcacgccgtt gcggtaacgg
    12241 gttagcgtgg ctatcacctc acccagcggt ttatcgctga agctcaggat gtccttcgtc
    12301 cagcttgtac tctcgtcatc cagcggtttc actgcgccaa actcagaggc gctgaactgc
    12361 aggctttcac cagcgttcac gatgcgtttt tgcgcggggg cactggcgag aagcacttcc
    12421 acagcgtgct gctgaacgtc aagctgcgtg aaattatcct gctggcggac ggtaaattct
    12481 gtccctaaag cagtgagctg gccctgacgg gtcaggacac ggaaggggcg ttgcagggca
    12541 tctttcgcgg tagtaatggc gatttcaccg taccagagcc ggacggtgcg ctgatgcgca
    12601 tcaaaacgca catccgccgc gctttgggta ttcagcgtga gcagggagcc atcttccagt
    12661 tgctgacggc tgactgtgcc tttggcggtg cggtaatctg cccgcagacc ttcgccggtt
    12721 tccgactgcc agagctgcca gcctccgcca gcgccgagca acagcagtaa tcctttcatc
    12781 acgtgacggc gggtgaggcg ggtatcgtgc aacgcccggc tggcaacgtc gccaggcaca
    12841 ccgccaagct ggttgcgcag gttttcaacc tgctgccagg cccactggtt atcctgatcc
    12901 tgttcatacc actgttgcca gcgcgcttcc tgttgtgggc tgacgcgctc gccgcttagc
    12961 acggcatacc agtgggaagc tgaacgcagc gcctgacggc gggaatcggt taacaaagga
    13021 ttcataaccc atactccaga cggaacagca ggcagtgctc gacggctttc gccacgtatt
    13081 ttttcacgga gctgatggaa acaccgagtt tgtgcgcaat ctcgctgtat gtcagaccat
    13141 ccagttgcga aagcagaaac gcttcacgtg ttttgccgtt tagcccgtcc agcatgctgt
    13201 cgaggagttg tagggtctcg agttggcttt cgcgttcctc aggtgaaggc gctcccccct
    13261 ccggcataag cgccagcatc tccagatacg ctttttccag cgcgtttcgg cgaaacaggt
    13321 ccaccatcac gcgtttggcg atagtgcaga ggaaggagcg aggatcgcgg atcgtcgaga
    13381 gcgtttcgct gaccattacc cgcaaaaaag tgtcctgggc aatgtcatct gcatcaaaag
    13441 cagactggag tttgcgcgtc agccagcttt tcaaccagcc gtgatgtgtg ccataaagcg
    13501 actcgaacgt taaggaagct gtggtagtgg cgcggtcaga catgcggagt gcatcaaaag
    13561 ttaattatca cgtagtcata ttaatatgag aatggttatc attacaattg gaaataaaat
    13621 tgtttccaat agacattttt aacatgttgt ttttctaagt gttataaggt aggtataaaa
    13681 tgggatggag cctctgcttc tgacatgtgt cggtcagaat gactcatgat gtggtctgct
    13741 attattgaca tcctcactgc cctaaaggat ggggatttcg gtaatgatct tacccagcaa
    13801 tagtggacac gcggctaagt gagtaaactc tcagtcagag gtgactcaca agacaaaaca
    13861 gtttcaacca gtaaaaaaac gtaaacacca ttcgcatgaa tttcgtagtt aagatctgaa
    13921 gcttgcagaa tgcatcggtg ttactgccgg agtccgtgaa cttagtctgc atgaatcacc
    13981 acagctttac atctgtcgca gtaaacagca aaatcagcaa acttacttaa ttatatcgag
    14041 tgcgattaca atcgctggcg atggtacagt tggtgtggcg gtctcagtcc ggaacaattt
    14101 gaaaatcata atctcactta aaacggcatc cattttacaa aagatggccg tctgtgtatt
    14161 tgtaatgaat accttctaca tgtttagagt gcataatcca cgacaaggcg atatcctaaa
    14221 aattagctag caggcaactt tgatggcata gataatgttt ttagtaaaaa agagttgact
    14281 gaatataata cagcctcttc ctacataaga aggaatgtgc tcacaaaaaa aaaccattag
    14341 tctgcttcct gttcttacaa taatcagtat tatgtatttt atttatatag caaaatatgc
    14401 ttgttacacc tgtcataatt aggcatggta aaattattga ctgaaccctg ggggagaaag
    14461 atgcacagtg attattataa tatggttttt ggtgaaaaat tggccaatat tttatatgaa
    14521 gctaactctc aattttttta cgagagaaat gtcatagaag aggcagtcaa cgctttattc
    14581 tgtgagcgtg atataataaa caacaagaac ataataaaga agctgatgtt ctttttgtca
    14641 gatgttaatc acaccaagaa agatgttgtt cagagcgcac ttaatattat cattgacatt
    14701 acatcaggtg atatttgaga ttttacataa aagataatct ctcaaggagt cttatctgaa
    14761 aacgaaatat atagtccctc tcgttttgta atatcgattg gctttgttga atcggttacg
    14821 taccccggag ccggtcatat tttcaggtaa tacacacgct ttcagacatt tcggctttcg
    14881 tcatttttgt atcatgggca tagcttctgc tacctgacca tcataatcac gcagcttcag
    14941 tgaagcagtg accacctgtt taacccggtg tatcgttatg atcttatcca gcaattgaac
    15001 cgccccgggt ttcctggaga gtgttttatc tgtgaactca ggctgccaga tcatcgtttc
    15061 cgatggaagc ataataagct ttttctgctt ctgccggtgg gatatggccc tgcctttcca
    15121 gcaatcgtcg attgttatac cagtccaccc acgtgagtgt ggtcagctca tcatactgac
    15181 acgtgcatta cccctaccgc gataatgcaa ccgtacaata tagggcgttt cctaaacgca
    15241 gccctcatca ctccaatcca acaagacaca gcatttttga tcacgaagaa agacaaataa
    15301 tttccgtaaa tgtttccgtt gttacaaata acaacagtgt tttacatttc tttggcctca
    15361 ggcccgcgca tgttgccatt cgatgtcatt ttcctgactc gtttcttctt tatggtgtag
    15421 tgtgatcttc actttataga tgctaacaat atgaaaagaa aaattattcc cgtgctaatc
    15481 ggttgtacgc tctctttctc tgccctggcg gcacaaccta cggctgagcg ctatgtcgtc
    15541 agttttccag aaggcacgca cgtgaactac gccggcgcat ttgccagtgc attcccgaac
    15601 ggccttccgg ttgggatagg ttctggcctg ctgttcaccg gtaaacaggg cgatgccctg
    15661 acattcgcaa ccattaccga tcgcggtccg aacgcggatt caccgaaaga ggggaaaaac
    15721 gaaactaaaa ttttcgttac gccagatttc gcgccgctgc tgatgacgat tcgtgtacaa
    15781 aacggcaaag cggaggccat cgatccccgt cctctgcatg acgataaagg cgcgatcaac
    15841 ggtctgccgc tggcaagcga cgtgattggc tccaccaatg aagtggcctt cagcgacacg
    15901 ttacatagac tgaaggggga taatcgcggg ctggatacgg aagggatcac tccggatggt
    15961 aaaggaggtt actggctgtg cgatgagtat ggcccgttcc tgattaacat cgacagtaaa
    16021 gggaaaatcc tggcaatcca cggtccgcag gcggcggaag gggaaaaagc gattgcgggc
    16081 ggtttgccaa atatcctcaa atggcgtcag gcgaaccgcg gctttgaagg cctcacccgt
    16141 atgccggacg ggcgtattat tgtcgccgtg caaagtacgc tggatatcga cgctaagagc
    16201 aagaaaaagg cgctgtttac gcgtctggtg agcttcgatc cggcaagcgg aaaaaccgcg
    16261 atgtacggtt atcctatcga cagcgcggcc tacagcaaaa acagcgacgc taaaatcgga
    16321 gacatcgtgg cgctcgataa ccagcacatc ctgctgatcg aacagggaag agataaaaac
    16381 aacagaatgc gtaacctgat ttacgaggtc gatctcaata aggcgagcga cctgtcaggc
    16441 ttcgacaagc cgggtgagta tccggagttt gatgatgaga aaaccctgtc acagcgcggc
    16501 attacccttg cgcagaaaac gcaggttgtt gatctgcgct ccttgggctg gcagcaggag
    16561 aaggctgaag ggttggcgct gatcgacagc aaaacgctgg cggtggcgaa cgataacgac
    16621 tttggcgtga aggtggcgat gcaacatccg gtcgagggca agacattcaa ggattatcgg
    16681 gtgaacgcgg aaggaaagct gacgcttgac gacaagcagg ttgaaacgac cctcagagtg
    16741 aagccactgg agaaaccgga gtctgacagt gaactgtgga ttgtgacgct gccggaagcg
    16801 ttgaaataag tttataaggt acggacagca gtatcgctta gtttttcaaa ttcatgtatg
    16861 tgaacaatat tggggttccg gcgtagaccc ccaatgaaag cgtcaccacg tgaaaacatg
    16921 cctgcacggt ttacacgtca tcccatggtg gcagccggtt aactttcctg cgccaccagt
    16981 ggaaaaaggc cataagcagt ccgaacaaaa gtaagcgtac agcgagggcc gtattgacgg
    17041 ggatgtgtta ttcagctggc agtgctatgc gccacggaag cagttcgctg acccggttga
    17101 ccggccagtc tgctatgacg ccaagcacat ggcgaaggta gctttctgga tccacgtcat
    17161 tcagtttgca cgtcccgatc aggctgtaca gtagcgctcc ccgctcacca ccatggccag
    17221 agccgaagaa cagggtcttc atcttttcac gcaaccagct ttccagggat ttcaacagcg
    17281 gtttcgtttt tcgctgacgt tcagcaagcc gctgctctgc cggcattccc cttatatccg
    17341 cctctatggc gtacaactga ccgatctgct ccagggcttc ttccgtcagt gctgacggga
    17401 tgcggacgtg cacatcgtgg atctttcggc gggcatgagc ccagcaggca gcttccgtta
    17461 tcccaccatt gcgatacagc tcgttgaacc cggcgtacgc atccgcttgc agcacaccgc
    17521 tgaagcaagc aagatgagtc tgcggatgga tgctttttct gtccgggctg taagcgaacc
    17581 acactgcagg tgccaacgct gacccggcat tgcggtcatc acgaacatac gcccacaacc
    17641 gcccggtctt cgtcttctta ttacccggca gcagtacctg gaccggggta tcatcggcat
    17701 ggagtttgcc gtcagtcatg acatagccat gaagcgcctc ttccagcgga gacagcagcc
    17761 ggcagcatgc atccacccag cccgacagca gtgaacggct cagctccaca ccttgccggc
    17821 cgtatatttc tgactggcaa tacagcgggg tgtgctctgc atacttcgag gtcagcacgc
    17881 gggccagcag ccccggtccg gcgatacccc gctcgatggg ccgcgaaggt gcaggtgcct
    17941 gcacgatggt atcgcactga gtacaggcat gtttttcccg taccgtccgg ataacccgga
    18001 aggcgctacg catcaactcc agctgttcgg cggtatcctc gcccagatag ctcagtgaac
    18061 cgccgcagtt cgggcagcac ggcgccgcag gcaacagtcg cttttcgtca cggggtagtg
    18121 attcagggaa cggcttacgg gtgcgggtct gacgcaacgg acgctgtact gccgggtcat
    18181 acaccctacc agtcagcgta tcgctctctt tctgaagccg gttcagatcg gcttccattt
    18241 gtgcgatacg gcgggagact ttttcggaac gactgccgaa gttcatccgg cggagtttat
    18301 ccagctgcgc ctgcagatgg tctatttcgc gctcccggtt gctcagcttt tcctgcaggg
    18361 cgtggatcag cgcttcctgt tcggccaggc gctgtttcag caggaagatg tcgtcagaag
    18421 agatgtcgtt cataagcccg tattttaccg ggcttattct gtgacaacca ggataaagag
    18481 atttacagca tggtcaggga ggtcagcagc cgcttgggct gtcgccagtc gataccttcc
    18541 agcagcatcg ccagctgcgc ctgcgtaagg aacactttgc catcacgggc tgacggccag
    18601 gcgaagcgcc cacgctccag ccgtttggtc aggaggcaca gtccgtcgcc ggtggaccac
    18661 agcagtttaa cctgactgcc gctgcggccc cggaaaatga aaatatggcc ggacatggga
    18721 tcgtctttca gcgccgtttg tactttcgca gccaggccgt tgaagctatt tctcatatcg
    18781 gtgataccgg caaccagcca aattttggtc ccggaaggta acgggatcat cgcttcagtt
    18841 cctgtatcag cagagtcagg agcttttcgc tgacattgcc attgaagcgg agcgtcccgt
    18901 gccggaacgt tacctcacag ctgatactga gggtttccgg gtcctctgcg agcgattctg
    18961 gctgttcggc agctgcatcg agagtcacag gaagtagctg ggggctctct gaagaaggta
    19021 atagcagctt tccctcgcgc cattgttgtc gccatttgaa caacagattg gcgttaatgc
    19081 cattttcaag agcaagtttt gagatggata tcccgggttc acaggaggca gcaacgagca
    19141 gctgtttaaa ttcgggagga taattagggc agccttttcg cctgccggga gtcacatttt
    19201 tctgcatatc tgacactttg gttcccacta cttatttggt ggacaccact ttgtctaatt
    19261 cgtcagattc tgaccagacg gttcaggctg tacgcttacc actattttat ccctgttgaa
    19321 tttggttaag ccggtgcaga cgcgccggga aacgcacacc tgaaccggca caataaaata
    19381 caatacaacc ccctgttact ggagatacga atggaatgac gaggcagcct gtcagtcaca
    19441 gggggctgtt ggcataaaat caacggaaag gccaccaaca ccggaattaa gctccacagt
    19501 ccccgcactg gcaatccatt cccgggaaag gcatcaccgc aaactgctcc ggaggcaacc
    19561 attacagcct ctgtaacgga ttatcagccc tggcatcttc cgacggcacc tcctgccgcc
    19621 cccacgccct gctgtatacc ccggtaccgc agcgtgggca gcacttttcc ccctcataat
    19681 cttcgtcaca catcacacaa aaccactttc tcaccttcac cgtataatcc ggaaccgtca
    19741 tgccctgcag atgcaaacga cgggcctctt tctctgccgg gtcaagccag gtacatcgca
    19801 gcaggatttt ttcagcgaca gtcatctggt catgttcaaa ccacggtggt tgtccgtcat
    19861 tgccccgctg aagtgttaac gggcatccac aggacacaca gcacaaaacg tcctgtcggt
    19921 tgtgcttcgc agcttctttc gccgtaacaa aatggccttt gctgtcattc gccatataac
    19981 agttcagaat tcgcatattt cctcctgagt tgtccgggga atactgaatc ctccggtctg
    20041 gcagtgtgtg cagtactgct ccccataata atcgtggtga cattgtctgc agtgccagct
    20101 ggctttacgc accacgggta aggcatccgg tacgaattgc tgcagacgtt taatcaactg
    20161 tatttctctg cgctccggcc tgacataagg gcactgttga ccgtgctctg tcagcctgtc
    20221 gtcagtgtgt tcaaaccagg gaagttcagt ttcgtattgc ggatggtatc tgagtgcact
    20281 gccacacaag tggcaggtgt agttagcata aggtgcggcc tgtgcagtac gggcgcccgt
    20341 cagacgtccg ttgccatcaa gagcgataaa ggattttgcg tacatagtca tatacctcag
    20401 ccgccagacg acacgcaggc aacagccgtc ccggtacggg cagcgtggtc agggtgtgaa
    20461 tggcggttca gttaaaatct ggactggagt ttgggtaaag caggctggtt gctgttacag
    20521 cactgtggtt gtcacacaat ggaacactga cgtaaaaaac agtcagtggg cgagtggata
    20581 catcgttgcc gccagacaac aacccatact tttggtttgc cggatattgg cgcgccagtc
    20641 gtgctcaact ccggctacag ccaaaattgc gctcaccggt gcagtgagct ttggtatgct
    20701 ccttcgccag atagtcagca cgttccagca cttgctgaaa gccggtgtca tcattacgcg
    20761 ccagccacac cgccggccag gccggaaaat gcaccagcgt ggcatggcac ccgacatcca
    20821 ctcccagggc actgcaccac gcctgtttaa tcatcccggc cagagactcc ggagcgcggt
    20881 aatcaccggc acgacaccag gtatcccggt tgaccagcag catcaggtga tagtgttttt
    20941 tacctttgca ctccccaaac tcccgggccc agacgtaatg cagggttgtg ggatgcacgc
    21001 gtttaccttc ccgatgctta cgtttctggt aagcgtcgat tcgggctttc agggcattga
    21061 tgaagcggga tatcacagct gcgtccgttg ctgccggtac atccgggaga cgcaggtcaa
    21121 cccgcaatgc cgtcaggcgg ggatgaacat tcagtgcgtg ccgcaccgtc tcacgaatac
    21181 gttgctgcca gaaggggttg tatttgtagg tcatggttaa atctccgtat ggttcatacg
    21241 gaatagccac gtcgtaaaaa atacgcagag tccctgaagt gacctccggc aggacactgt
    21301 ctcaggcact ctgtgataac ccgaacggtc gtctccggac tgacggttga attttcagcg
    21361 tcgtttttta atgtaaaaaa ccggttacgg gtaaggctgt gaggagggag tgatggtgat
    21421 acgcaaaaag aagtgcaggg actgtggaaa cgcgattact cataatacag tgtgttgccc
    21481 gtattgtggt tctgtcgacc ccttcggcta ctaccggaat acagaccgta tcgttaccat
    21541 actgctggcg ctgattattg tggtcctgct gactactgtc agcgtcagtg tgtgtatact
    21601 gtgtagctgg tgagataacg gttcatgtat cccctgagct gacagtgtac cgtgtatatt
    21661 aagcgtaacc gacaggttct ccttcatacc atattaaatt ttccagggat tgccgctgcc
    21721 ttatttcgtc ccatacagga aaactcatct gggtttctga caaatacgcg aaactcacaa
    21781 cccctcaata tcaaccacac gttgtaagcc ggtaatacct gaaaatcagc ctattaccgc
    21841 ccacgcctcc ggcaccattc ccttttgatt tcgaattctt tgttgtcgct ataccgctta
    21901 atgttcccgg tcctccggtg cagataacac cggagtcatc gtagcgctgc ttcaatattc
    21961 aggtgacgta tttccagacg gaagccggcg cgcgatcgta aagtttgttc attgtcagtt
    22021 ctgccagccg gttgtcaacg gcagagttaa aggcttccag ctccgcaagg gtaagtttat
    22081 aacgcgtgtg ggaattcact tttcccgcgt ctcctgacgt attacgggct gccacttacc
    22141 tggtgtcccg caatacagcc ttagtgttcc gttatcgggg atccggacgc accgcaacaa
    22201 ccggggaagt atttctggtt tagagcagtg tcaagaggga tttctcagcc cctcactcac
    22261 cggctcagtc tcagggcagg agcattaata cgctcagttc atgtcttata caggaatggg
    22321 cacaggggga gctggcgtgt cactgctgat tttacgcatc agcgttcatg tatctgcact
    22381 ctgatacatg aacgattcac agcgctctgt taccggagag atttactctc ttagcttact
    22441 ctggcaaatc ctttacgtta ctctctgatg acttcttata tttataatac caatcagaca
    22501 gccttatccc at
//
LOCUS       DAEEUE010000061        22434 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.061, whole genome shotgun sequence.
ACCESSION   DAEEUE010000061 DAEEUE010000000
VERSION     DAEEUE010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22434
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.061"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..832)
                     /gene="rfbG"
                     /locus_tag="KLK84_002543"
     CDS             complement(<1..832)
                     /gene="rfbG"
                     /locus_tag="KLK84_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HBE2009820.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     L"
     gene            complement(825..1715)
                     /locus_tag="KLK84_002544"
     CDS             complement(825..1715)
                     /locus_tag="KLK84_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HBE2009821.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(1745..3001)
                     /gene="wzx"
                     /locus_tag="KLK84_002545"
     CDS             complement(1745..3001)
                     /gene="wzx"
                     /locus_tag="KLK84_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HBE2009822.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGIYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYKINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(3017..3562)
                     /gene="rfbC"
                     /locus_tag="KLK84_002546"
     CDS             complement(3017..3562)
                     /gene="rfbC"
                     /locus_tag="KLK84_002546"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBE2009823.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(3567..4445)
                     /gene="rfbA"
                     /locus_tag="KLK84_002547"
     CDS             complement(3567..4445)
                     /gene="rfbA"
                     /locus_tag="KLK84_002547"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBE2009824.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(4504..5403)
                     /gene="rfbD"
                     /locus_tag="KLK84_002548"
     CDS             complement(4504..5403)
                     /gene="rfbD"
                     /locus_tag="KLK84_002548"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBE2009825.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(5403..6488)
                     /gene="rfbB"
                     /locus_tag="KLK84_002549"
     CDS             complement(5403..6488)
                     /gene="rfbB"
                     /locus_tag="KLK84_002549"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBE2009826.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGNQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            complement(6861..7754)
                     /gene="galF"
                     /locus_tag="KLK84_002550"
     CDS             complement(6861..7754)
                     /gene="galF"
                     /locus_tag="KLK84_002550"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HBE2009827.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(7929..9323)
                     /gene="wcaM"
                     /locus_tag="KLK84_002551"
     CDS             complement(7929..9323)
                     /gene="wcaM"
                     /locus_tag="KLK84_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HBE2009828.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRCSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(9334..10554)
                     /gene="wcaL"
                     /locus_tag="KLK84_002552"
     CDS             complement(9334..10554)
                     /gene="wcaL"
                     /locus_tag="KLK84_002552"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HBE2009829.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(10551..11831)
                     /gene="wcaK"
                     /locus_tag="KLK84_002553"
     CDS             complement(10551..11831)
                     /gene="wcaK"
                     /locus_tag="KLK84_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HBE2009830.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(12011..13489)
                     /gene="wzxC"
                     /locus_tag="KLK84_002554"
     CDS             complement(12011..13489)
                     /gene="wzxC"
                     /locus_tag="KLK84_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HBE2009831.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(13491..14885)
                     /gene="wcaJ"
                     /locus_tag="KLK84_002555"
     CDS             complement(13491..14885)
                     /gene="wcaJ"
                     /locus_tag="KLK84_002555"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HBE2009832.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVVGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(14940..16310)
                     /gene="cpsG"
                     /locus_tag="KLK84_002556"
                     /gene_synonym="manB"
     CDS             complement(14940..16310)
                     /gene="cpsG"
                     /locus_tag="KLK84_002556"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HBE2009833.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWCFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(16503..17939)
                     /gene="cpsB"
                     /locus_tag="KLK84_002557"
     CDS             complement(16503..17939)
                     /gene="cpsB"
                     /locus_tag="KLK84_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HBE2009834.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(17942..19165)
                     /gene="wcaI"
                     /locus_tag="KLK84_002558"
     CDS             complement(17942..19165)
                     /gene="wcaI"
                     /locus_tag="KLK84_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HBE2009835.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQSGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(19162..19641)
                     /gene="gmm"
                     /locus_tag="KLK84_002559"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(19162..19641)
                     /gene="gmm"
                     /locus_tag="KLK84_002559"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HBE2009836.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(19641..20599)
                     /gene="fcl"
                     /locus_tag="KLK84_002560"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /pseudo
     CDS             complement(19641..20599)
                     /gene="fcl"
                     /locus_tag="KLK84_002560"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
     gene            complement(20602..21723)
                     /gene="gmd"
                     /locus_tag="KLK84_002561"
     CDS             complement(20602..21723)
                     /gene="gmd"
                     /locus_tag="KLK84_002561"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HBE2009837.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(21749..22297)
                     /gene="wcaF"
                     /locus_tag="KLK84_002562"
     CDS             complement(21749..22297)
                     /gene="wcaF"
                     /locus_tag="KLK84_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HBE2009838.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(22313..>22434)
                     /locus_tag="KLK84_002563"
     CDS             complement(22313..>22434)
                     /locus_tag="KLK84_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HBE2009839.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT         5648 a         5862 c         5097 g         5827 t
ORIGIN      
        1 ttaaagccaa gatcaaattg cctatataac ctttacccaa ttgattattc acaaagctat
       61 catcagctaa ggcattagct attttagtta cttcttttcg ataccacttg tttctcacca
      121 aaccaagccg tttgtatttt gctttaaaac taatgtttgc tcccacttgg ttgttttcat
      181 gctgtctgta cagcattgtt ggttttctat caatatacca cgaataattt ctcgtccgcg
      241 caaatgaata taaaaaccaa tcatgcagac agatatcttg tgcagcattt ttattattta
      301 tgatgaactt cttaaattca ataagcgttt ctttttttat aacatatgta caccccggac
      361 ctgctgcttc aaaaaaataa tcgaattgag tttgcgactg agccttatca actaaatttt
      421 ttcgccctga tggataatat gcaattacat tgctggaata acaattcgca ttattaaata
      481 ccaagcagtc aattgcattt tttattttat ctttatacca aatatcgtcc tgatcactaa
      541 aacagatata atcatagttt tcaagatcga catctttaat taatctataa aaatttttac
      601 ctgccccccc aaacttattg acgcttggaa gtatatttat aattgaggga tatgataatt
      661 gaaattcttc acaaatattt aatgtattat cagttgataa atcatctgat ataaaaattg
      721 agatgtctac atctttttgt tcaataatac tttttaattg ttcctcaatc cagcattcgc
      781 cattatacgt agccataagt acagctactc ttttttgatc agaactatgc attataacga
      841 ccgcccccag taattcctct tattccatcc agcgtagcca aaatatgttt tttcatatat
      901 tcaattttat ttttttgagt tgcgataatt aaagtcgtat gaattaaaga aaaaacaact
      961 tccctaatag agagtaaaag aggcacatga ttttttctaa gcaaaagaaa tgcatttctt
     1021 actcgataat aacgacgtac tggcgagtga attggtatgt taatcccaca gatttttact
     1081 gacttatccc caatagcatg aaccatttct atatcaggaa tgatatgaac taatatgcca
     1141 tagtttaagc atctaagaca ccattctgta tcgacataat caataaaaag ttcctcatcc
     1201 atcattccaa caatctcaaa aacttctttt gaaaccatgg ttccggatga gattgttact
     1261 gaacttttaa acggtgtctg tccttcagtg acatgaattt tctctcgaag accatttttt
     1321 ttgatattac aaataggata ataatgtgac ttatctctat caataaaaac tgggccagct
     1381 atttttgcat tttcattatc catatagcag gaaagttttt tgataaactc attgcgaata
     1441 cggctatctt gatcaaaaaa aattatcttc tctgcccctt tttcaagaga atatcgaatc
     1501 ccaatatttt gtgcttttgc tataccttca ttcctaccaa gacaaattaa atgaactttt
     1561 tcaatgtctg ctaatttatc tttcaaggtg aaattagtcc tattatcgac tactacaacg
     1621 taacaatttt gctcctttag ttcagtgatc agtgcattca gatttttaag ttcgggatta
     1681 tatgtcacaa tgacagcgta aatattacta ttcattatcc aagtgactca gtaattggtt
     1741 aattttagtt ttcatataca gaacacattc tagtataaag ccattgtaat aacagagcat
     1801 ctaaaattac acgtatgctc caggatattg cggcacccag taacccatat ttttcgacca
     1861 taaaatacaa aagggctaaa taaggaacca attcagcaca atgaatcaaa gcagtaattt
     1921 tagattttcc taatgcctgg attgcagaga atggtatcat tgccagcgca ttgaagaaaa
     1981 aaccgatcaa taacacattt aatatattaa aggaattaat tttataattc tcaccaaacc
     2041 atattttcaa tacaagccct gaatataaca agcctatgat tatcaccggc aaacagatta
     2101 gaaacattaa aagcaatgat ttatttacat tacgtttaaa ttctttaaag tctttgatgt
     2161 tacttaacct tggaaacact gccctcccga ttgctgcagg tattattcct aattttagaa
     2221 taacctccgc tggtgcagaa taaaatgcaa ctttatctgc ccccataata tttgacacta
     2281 taaatcgatc aaaatacacc atgaccggac ttatgatatt acttactgtc atccaaccgc
     2341 caaagaaaaa gagtcgttta aaggttttat aacaaaaatg aacccccgaa attttaatat
     2401 cattcctgac ataataggca gagactaaaa tcgaaataac tcttgcaaat attaaaccag
     2461 caaccgctgc cgacaatgta gcactataaa aaacaaatat ggccggaatt cccgcgatgc
     2521 aagagcttga tatagatttt tgaatattta caatgccaaa tttttcatcc ccctcaagaa
     2581 tggctgacca taattgattt agaataaata atggaatgca aatagccaac agtttaaatg
     2641 ctagttgtat atcactatgt tcaacaccag aaatttttag ataattaaca attccatcag
     2701 agaaaatcaa taataaaaaa gcaccaaagc atgaaaatag caccaaaaaa gatgtacttg
     2761 ttgaaattac cttggttctt tcatgatgat tatcacgatg aattgcaatt tctctaataa
     2821 cagagcgagt caggcctaca tcaaaaattc cagcataccc aacaagtgca attgcaattg
     2881 tataaatacc gaaaccttca ggccccaaac ctcgagcgag aaatcctagc gaggggatag
     2941 caattaaagt tggtattgca tagccaaaaa ggttccagac actatttttt attatgctca
     3001 ttattacatt tctttatcat ataaaataat cagcatctac aaatttttga ccattaatat
     3061 ctttatcaga aagcaatgga ttttgaaccg gccattctat atttatactt ttgtcgctcc
     3121 ataaaatccc acgctcttgc atttttgaat agaaattatt tgttttgtag acaaattctg
     3181 cttcatcact aagaacgcaa aacccatgag cgaatccttc cggtatccat aattgacgct
     3241 tattctccgc cgacaaattt acaccaaccc acttgccaaa tgttggtgat gatttccgaa
     3301 tatcaacagc tacgtcaaaa acttcaccga caacacagcg taccagtttt ccttgagcat
     3361 aaggttccag ctgatagtgc agtccgcgta acacaccctt agttgattta gaatggttat
     3421 cctgaacaaa ctcaaccttt cgtcctacag cctcttcgaa aactttctga ttgaagcttt
     3481 ccataaaaaa gccccgctca tcaccaaata cttttggttc aaaaattagc acatcaggaa
     3541 tttcagtttt aattacgttc attttattaa taacctttaa tcattttcag cagatactga
     3601 ccataagcat ttttcttcag cggctcggct aataccttca cttgctcagc atcaataaag
     3661 cctttacggt aagcaatttc ttccgggcag gaaactttca gcccctgacg ctcttcaatt
     3721 gttgcaatga agttgcttgc ttcaataaga ctttgatgtg tccccgtgtc cagccatgca
     3781 taaccacgcc ccatcatagc gacagacaaa cgtccttggt ccatataaat acggttaata
     3841 tcggtaattt ccagttcgcc acgtgcagat ggtttaaggt ttttcgccat ttctacaacg
     3901 tcattgtcat agaaataaag cccagtaacc gcatagttac tttttggttc cagcggtttt
     3961 tcttccagac taattgccgt accgttatca tcaaattcca ccacaccgta gcgctcagga
     4021 tcatttacgt gataggcaaa aaccgttgcg ccactctctc tgttaacagc ggtatccatt
     4081 aatttcggca ggtcgtgacc atagaagata ttatcaccaa gtaccagcgc acaatcatca
     4141 ccacctataa attcttcacc aatgataaat gcttgcgcca gaccatccgg actcggttgt
     4201 actttatatt gcagattaag cccccactgg cttccatcac caagcaattg ttgaaaacgt
     4261 ggtgtatcct gcggcgtact gataatcaga atatctcgaa tacccgccaa cataagcgtt
     4321 gaaagcggat aataaatcat cggtttatca taaatcggta gcagttgttt gctcacagcc
     4381 attgtcacag gataaagacg agtgccggaa ccaccagcca gaataatacc tttacgcgtt
     4441 ttcatttcac cattcctttt aattcatctc gctctagcat catgagcgag atgcaataat
     4501 ttgttaaatt gccgtggtcg taaataattc gttgagcata cgtttcacgc ccacctgcca
     4561 gtcaggcaag acaagcgcaa agttctgctg aaatttttcg gtattgaggc gagaattatg
     4621 gggacgacgt gctggtgtgg gataggccgt tgtcggcacg gcattaagtt tgttaagagc
     4681 aaggttgatc cccgctctgc gcgcttcttc aaataccaga gcggcataat cgtgccaggt
     4741 tgttgtacca ccagcaacca gatggtacaa accagcaact tctggtttat ttgcggccac
     4801 gcgaatggca tgagcggtgc aatcagccag taattcagcc cccgttggtg cgccgaattg
     4861 atcgtttatt acagccaact cttcgcgttc ttttgccagg cgcaacatcg ttttggcaaa
     4921 gttatttcct ttaccagcgt atacccagct ggtacggaaa ataagatgct ttgcgcaatg
     4981 ttcttgtaac gctttttccc cagctagctt ggtttcaccg tagacattta gcggtgctgt
     5041 tgcatccgtt tccagccatg gcgtgtcacc gttgcctggg aaaacataat cagtggagta
     5101 gtgtataacc caggctccga cttcattagc tgcttttgca atcgcttcga cgcatgtcgc
     5161 gttaagtaat tgcgcgaaat tcggttctga ttccgctttg tctactgcag tgtgagcagc
     5221 agcattaaca ataacatcgg ggcgaatttt tttgacggtt tcagccacac cttcggggtt
     5281 gctgaaatct ccacaataat cagtggagtg aacatcgagc gcaatcaagt tacccagagg
     5341 agccagagca cgctgcagtt cccaacccac ctgccctgtt ttgccgaaaa gcaggatatt
     5401 cattactgac ggccctcata gttttgttca atccacgact gataagcacc gcttttcaca
     5461 ttctcaaccc aatttgtatt agccagatac cattccaccg ttttacgaat cccgctctca
     5521 aacgtttctt gcggtttcca gcccaattcg cggctaattt tatcggcatc aatggcgtaa
     5581 cggcggtcat gccccgggcg atcggcaaca taagtaattt gctcacgata agatttctct
     5641 ttcggtacaa tctcatccaa caaatcacaa atagtgagca ctacgtcgat gtttttcttt
     5701 tcgttatgtc caccaatgtt ataagtttca cccgctttac cttcggttac gacggtatat
     5761 aacgcacgag catgatcctc tacatacaac cagtcgcgaa tttgattccc tttaccataa
     5821 ataggtaatg ccttaccttc cagagcattc agaataacca atggaataag tttttccggg
     5881 aagtgataag gaccataatt gttcgagcaa ttagtcacaa tggttggtaa accataagtc
     5941 cgcttccagg cacgtaccaa atgatcgctg gaagctttag acgcagaata cgggctactt
     6001 ggcgcgtaag ctgtcgtttc cgtaaatagc ggtaacgctt cgttattatt tgcttcatcc
     6061 ggatggggta agtcaccata aacttcatca gtagaaatat gatgaaagcg gaaacttttt
     6121 ttcttttcat cattcagagc agaccagtaa ttgcgcgccg cttctaaaag aacataagtc
     6181 cccacaatat tggtttcaat aaatgccgca gggcccgtta ttgaacggtc aacgtggctc
     6241 tctgccgcca ggtgcatcac cgcgtctggc tggtgctgtg cgaaaatacg cgacatggct
     6301 acagcatcgc agatatctgc atgctcaaaa gcataacgtt cagaatcaga aacatcagcg
     6361 agtgattcca ggttcccggc gtatgttaat ttatcgacat taacaacact atcttgcgta
     6421 ttatttatta tgtgacgaac aacagcagaa ccaataaacc ctgcgccacc agtaacaagt
     6481 atcttcactt ttttattcca tatagctata gagcatgctg tgaaatagac tgctccagat
     6541 ttgattaata gaggtattaa tacacgctac cgcccctggc ttaacagcta ccagtgcact
     6601 gcatgcatct caacgatgtg gcgagcgtta gccactcgcg ccaaatccga aaaattcaaa
     6661 cgttaattgt cttaccaaac agccgtagaa acaaggaaaa tcctggaaaa atttgaataa
     6721 tatgagctat gtaacttctt gtttttatag gctttataaa aacggcaacg gcaggaaaat
     6781 tcaatgagca aaacttatgc cacgaatctt cactgccgtt ataattttct tatcaaccgt
     6841 tacatccggt cagattttca ttattcgctt aacagcttct caataccttt acggaacttc
     6901 gccccttctt tcaggttgcg taggccatac ttcacaaacg cctgcatata gcccattttt
     6961 ttgccgcagt cgtaactgtc gccggtcatc agcattgcat caacggattg ttttttcgcc
     7021 agctcggcaa tagcatcagt cagctgaata cgtccccatg caccaggctg agtacgttcc
     7081 agttccggcc aaatatcggc agaaagcaca tagcgaccta cggccatgat gtctgagtcc
     7141 agcgtctgcg gctgatccgg tttttcgata aattcaacaa tgcggctgac tttaccctca
     7201 cggtccagcg gctctttagt ctggatgacg gagtattcag agaggtcacc cggcatacgt
     7261 tttgccagca cctggctgcg gcccgtttcg ttgaaacgtg caatcatggc agcaaggttg
     7321 taacgtagcg ggtcggcgct ggcatcgtcg atcacaacgt ctggcagtac cacgacaaat
     7381 gggttgtcac caatggcagg tcgcgcacac aaaatggagt ggcctaaacc taaaggttcg
     7441 ccctgacgca cgttcataat gatcacgccc ggcggacaga tggactgtac ttccgccagc
     7501 agttgacgct tcacgcgctg ctcaaggagt gattctaact cataagaggt gtcgaagtgg
     7561 ttttcgaccg cgttcttgga cgcgtgagtt accaggagga tttctttgat ccctgcagcc
     7621 acaatctcgt caacaatgta ctgaatcatt ggcttgtcga cgattggtag catctctttg
     7681 ggtatcgcct tagtggcagg caacatatgc atcccgagac ccgctacagg aataactgct
     7741 tttaaattcg tcattatttc atccacctgt aaaatggttg ctgaattata gcttgttcga
     7801 tttttttcgc cagcatcaat taccctgaat tgattactga attacttgtg atgttacgcc
     7861 gcttcgttgt ggattgcagt agcattgttc ctaagtatgg ctccattttt ccaggaatgg
     7921 tcgcaaatct actccctccg ttccggcaat ctaaagttaa tcttttccac attaacaata
     7981 tggtgattaa tcctgtcgat atcgacggag ctttgccctc tttcattcac cgcatgaaca
     8041 tttgcaagag acagcagtgt ttcttttttc gccataaaga cgccgcgaac gtctttgcgc
     8101 atgtcgaagt tcatgctcaa tgctggtcca actgaggatt cctgcatcac attgatattt
     8161 cgcataaaaa gatgttgcgg cttgttgtgt aactccagcg aggcacgctt catctcaatg
     8221 ttagttagcg acacaaagga gacggcattc ccggcggaga tttggatgcc gcgcaattta
     8281 taagcaagat gggtattatc cagttgaata ttattcactc ggaaattttg cggtatcgag
     8341 agatatttgc ctttaattac cccatagccg attaacatcc cggcgctatt aatcatttca
     8401 atattatcaa tcacgaaatt gtcacaaccg taaatagcga ctgtcgcgtt atcaatgccc
     8461 gctttcttac tgaaatccgg cgtgatattg cgggcattga tattacgaat aacaaaatgt
     8521 ttaccatttt caacatggat caactgccga caatccgatc ccgtgatatt cgccacgaca
     8581 aagtttttca ctgcctggtc ttccgggtag ttgttatcat aagtgcttcc cgcaaggcct
     8641 atgccgatgc cccagttgat tttgccgttg gtacagttga tacgctcgat gacatggtca
     8701 gatatcaaaa tatcactgtc gttaattgcc acgttccatt caatggcgtc gccctgtaag
     8761 tcgctgaact tacagttggt aatgttggca ccgataatct ggttatgaaa tccctggcgt
     8821 aagatggcgt aattagcgtg gctgacagtg aggttatcga tggtcaggtt gcgcatgacc
     8881 cgtttgtttt tgccgccaat ataaatctgc attaccgggc caaagccgct catcgccagc
     8941 cctttgatgg tgcagtcaga acaacgcaca tccagggtaa tgttatgcat accgccgccc
     9001 tctccccctg tcacctggct gccgtcctgt aagacaaatc gccctctgcc gttgccgcgc
     9061 aggcttccaa ggatgtgtaa cgttttgccg ggagggatga agatgccggt gttgatattg
     9121 tcacaaacga atccggcagg cacgacgacc gtttgccctt cgctgaaggc ttgtttaaat
     9181 gaggcgatcc agtcatgtgg gttgtagtcg ttaatgttaa cgctttgtcg ggcgggaagt
     9241 gcgcgagcga aaggggtatg gaggaaggca agcgccgagc ttgccgtcag gaaggtgcgt
     9301 cgggagagtg ttttaaatgg catacgttct cctctataaa gcctgcagca agctggcgag
     9361 ttctcgatta atcacctgct ggttaaaatc gtgttcgact ttttcgcgcg cacgtttgac
     9421 gaccggagcc agttcgtcgg tatccagttg gctaaacgcc gccagacgtt gtgccagtgc
     9481 gcgagcatcg ttctcaggca gcagccagcc ggatttatcg gcctccacca gttcaggaat
     9541 tccgctatgc agagtagaaa ccaccggaat accgaccgcc atcgcttcca ttagcgccac
     9601 cggaatgcct tccatatcgc catccgcgcc cgttaccgat ggcaacagga agacatccgc
     9661 gtcgtcgagc atctccttca cttcatggct cggtttaaag cccggcatct ctaccacatc
     9721 ttccagttga tattgttcga tgagggtgcg caggcgtcgt tcccacaggc caatgccgag
     9781 gatgcgatag cgaaatgcca cgccctgctc tttcaactga cggcaggctt caatcgccac
     9841 atgcaggcct tttttctcgg ttaagcgtgc gacagatatg atttccagcg gcgttgcggg
     9901 cgctttcacc ggacgcgggc taaagcgcgt catgtccacg cccatacgcg atacggcgat
     9961 tttttctctg gggcagccca ttttttgcag ccttccggcc cacagatcgc ttatcggtaa
    10021 catcaggtcg ccacggcgaa acagttgttg atattcggga gtgtagtggt tgagcacttc
    10081 ccgactggag atatcaatac cgtggaagat ggtggcaatt ttgccgcgaa tgatacccag
    10141 ttcgcgtagt tttgctgcgg ttacccccgc tgggccaaaa tgagcgatga acacatcggc
    10201 gcgaaacggt gttgcgacct gaccacaaat ggcagacaaa atcaggttcc gcgactcggc
    10261 accatagcgt ttgaggttga gcgcatgcca ggtattttta cgatgaatgc cacgtaaggt
    10321 ctggctggcg cggtggcgca gtttcgctac tttgcccgca ggttcgtcct gtaaccagcg
    10381 ggttctggcg acaaggttgt atttcgtcca tgccgcgtgg gtgttctctg tatcgccttt
    10441 ttgcaaagcg acaatctcca cttcaaagcc catatcaata aacgcggtaa tttgattgag
    10501 gacgaaggtt tccgacgaca gcgggaattt caataagaag aagccgacct tcatttcacc
    10561 tccccgatgc gctcaagcac agactgcacc atctgcattc ccgtctgacg ttcgcgactg
    10621 acggcttcgt taaatcgagc gttcagcacc ggaagctggc ctaaggtatc cgcaaccatc
    10681 gcttgcaggc tgccgtccag taaatgacga atatcaatcg ccatctctgg cagtcccagc
    10741 tgctgcataa tcccggcgga tttatgttca tagttgatgg caatcgccgg agtagcaaag
    10801 ttcattgaga taatggcgga gtgcaggcgc gtaccgacgg tgagttcaca ggctccgaga
    10861 attttgccca tttccagatc gttgagttca tccatcacta cgtggtaacg ggcaggatcg
    10921 ctgatgtgct ggcgcagatt gagcgccacc atgcggtcgt ctttgttata gctgtcgata
    10981 cccgtacagg tggagagcgc aatcacctga tagccttcat cgagaatgcg attgaccacc
    11041 ccggcaaagg ctttttcata cgcttgttga gtggtgccga gacgtttgtc gaacggtgcc
    11101 agttcgcgca gggtaattgc cactgttttc tgttgtgcgg caacgtccag ccagtgttga
    11161 acggcatagc tggcggtgaa gtcttcggtg tggtgatcga ccagccacgc ggtatcgacg
    11221 ccatgttcca cttttgcggt ggtgatattt ctgcgtttca tcaaatcaag gctgaccgat
    11281 tcgcgcagga tcagcgcgtc gcagtgacca aagacatagt tcgccagttg gttaaattgc
    11341 tcatcctgga aggggccgac gctgtgacca atcataaaca gcggcttttt cgccataaat
    11401 gtgcaaagcg catgttcaaa ctgcggcacg ccgtagagat cgacaaaaaa cgatccgccg
    11461 acctgaataa tggcgtcgta acctgacagc aggcgcacga agtcggtgaa tccctgggcg
    11521 atggcgatat tgcgcagctt gccagtgtcg gtgacgcgtg agagcaatac ctgatgctgg
    11581 tagcggcgac ggaggacttt tttaatgcgc ccgacaacgc ccgccgcgct attgtgttgt
    11641 gtcatctgca ggaacagcgg atcgcccatt accgggcggt tgagcagcca ggaagaactg
    11701 accggatagc ggctcatcac gtccacttcg gtatgtggat tgaggatgtt gatggcatca
    11761 agtaagccgc gcaagatggc gctgtcgcca cgattgccgc aagtgtggtt gcccagaata
    11821 agtaatttca ttttttcctc ataaatttga tgccaggtga ggctgtgttt gttgtgttcg
    11881 ttgccgtttt ttgcctgatg cgacgctggc gcgtcttatc aggcctacag gtcccgagca
    11941 cagaaccgta ggacggataa ggcgttttac gccgcatccg gcaaccgttg tcggaaccga
    12001 aaacagcaat tcaccccgcc cgtaaaagca ttttcatttt ttcgctgcga caaaactgac
    12061 gcttcacttc caccaccagc ggatggcgtg acagcacaat catcaccaca aacgccagca
    12121 cgcccgcggc tatttgcacc gccagcagca tccccagcgc cagttgccct ttcagcacaa
    12181 tgcccagcgc ataactgacc accagcgtcg gcaacgagag ataaaacggc agccacaaac
    12241 tcaggatgta ctggcgataa ctggaaccaa gaaccggttt aatcatcacg aagtaactca
    12301 gaatggtgtt gataatctgc accagcagga aaccgagcgt gacgccgatt gcgcccgcca
    12361 tctgcccacc aataacaatc gccggaataa acagaaatgt tttgaatacg ttgaatttaa
    12421 agctgatatc gacccgcgct ttcgccatca gcagcgaacc aatcgggtta cccacggagc
    12481 gcagcagacc cacaatacac agcaattgca gcaccggaat aatgctgttc cacttttcgc
    12541 cgaataccag cggtacgaag ttattcgaca ccaccatcag ccccagcagc gccggaaagt
    12601 tgataatccc cactatcgac agcagcttgt agaagttaac acgcagcttt tcggtgtcgt
    12661 cctgaatttt ggcaaacgcc ggaaacaaca cgcgggtgat gattgggttc agcttcatcg
    12721 gcggtacgac ggccacgttg tacgccaggt tgtatccccc tgccacgccc gcgccaagaa
    12781 tacgcgccag caccagcgtt gaaaggttgg tgttgagata gttgatgatg ctgtccgccg
    12841 tcagccaggc accaaaacgt aagttcggtg ccacagacgc cagcgagaag tgcagaccgg
    12901 ggcaataaat tttgcgtcca aagtagccaa acagcagtgt tctcaccgca ctattgacca
    12961 gataaccgag gatcgcggtc atcgccagcg gccagaaatg ggcgctaact accgtaaaag
    13021 tgaagcccgc cagcaccgcg ctggtttcga tcatgccgat tttgttgaac tccagctcct
    13081 tctgcatcaa cgcgcggaac tgttgcccgt gggggatcac cacaaacgcc agcgacaatg
    13141 ttttaatcaa cggtgccaag tccgggttat tcagcacgtc gccgatgaca tcactcaaca
    13201 aaaacaccgc cacacatacc acaagcccca gcccgacgtt cagccagtac agcgtggtga
    13261 gttcaaggtg actgatttct tttcgctgaa taatcgagtt agcgataccg aagtcagaaa
    13321 gcgtatctgc cagcgcgata atcaccagcg acacagtcag caggccgaac tggtggttgt
    13381 cgataatccg cgccagcacg gtcatctgca ccagcccgag gccgatgatg atcaccgtgg
    13441 caatagccga ccacttcgcg ccgctgatgg ttttttcacg taagctcata tcaatatgcc
    13501 gctttgttaa cgaagccctt gaataccgtc aggaaaacga ttttgatatc gaaccagacg
    13561 ctccattcgc ggatgtactc aaggtcgaac tcgacgcgtt tttccatttt ctccagcgtg
    13621 tcggtttcgc cgcgccagcc attaatctgc gcccagccgg taatgcccgg tttcacttta
    13681 tggcgcagca tgtagccttc aatgagctgg cgatactgtt cgttatgcgc tactgcgtgc
    13741 ggacgtggac cgacaatcga catccccccg gtcagcacat tgataaactg cggcaattcg
    13801 tccagcgagg tgcggcgcag aaagttcccc actttagtga cgcgcggatc gttctgcgtc
    13861 gcctgggtca ccactttgtc gttctccatc actttcatgg aacggaactt ccacactttg
    13921 atcggcttgc catccatacc atagcgagtc tggcggaaaa taaccggccc tggtgaactg
    13981 agtttcaccg ccagcgcaat acagcacagc accggggaga tcagcagcag aataagcgtc
    14041 gccagcacaa tgtcttccgc acgttttagc aggcggttaa cccccgaaag cggcgtgtca
    14101 tacagcggca ccaccggaac gccgttcatc tcttcgagac gtgaatggag aatgttgaag
    14161 gtaaagacgt cggggatcag cagcaccgaa caggtggtat ccgccagttg atggaccagt
    14221 tttttcactc gtgcgccgtc gcacatttgc atcgcgatat agacgttatg aatcttgccc
    14281 gcttttgcat cctcgaccag ctgttgcagg ttacccgccc agtcgttaga aacgccgccc
    14341 ggtttcgggt cgtggtaaac gcccaccact tcaaacccta accacggctg attacggaaa
    14401 ctctccatca gcatttgtcc ggcggctaaa tcccccacta cggcgaccat ccgcttgttg
    14461 tagccatgat tacgcagcca gcccgcccca atgcgaatac atgaacggca aaccaccagt
    14521 ccgatgctgg tcagcccata ccacgccagc cagattttca gttgcgtgtc gaaatcattg
    14581 ttgaacgcca ccagtccggc gctgaaaatc acgcttaagg tccagttttg tagcaacagg
    14641 gcaaattctg tcgctgcccg aacaccgcgc catgagcgat aaaaatcggt gatgccgccc
    14701 agcatctgga acaccaccag cgtaatcagc gccaccaaca ggtgcatgta gaggaatgac
    14761 agtccgctga cttcgcaaac cagccatagc ccggcaaaca tgatggtgat atctgaaaag
    14821 cgttgcacca tagagattaa cgatgcattg gttttcgctc gctcgcgctt ttttagattt
    14881 gtcatcgttg ttcctgttat tagcccctta cccgcagtgg ggtaagggaa gatccgacat
    14941 tactcgttca gcaacgtcag cagagttcgc gttcgcgctt ccatcagcgg cacatcaccg
    15001 cgcgattcca cattcaggcg caccaccggt tcggtattgg aggtgcgcag gttaaagcac
    15061 cagtcggcaa aggtcatgct gatgccatcg ctgcgatcca ccgccagcgc ctcacggcta
    15121 aaatgctgtt ccacgcggtt aatcgcctca acgggttgcg ccagtttgct gttgatctca
    15181 ccgcttgccg gaaacgccgc catccggtcg cgcaccagtt cgcccagcgt ttttcctttc
    15241 aggcacacca gttcggcgac cagcagccac gggatcatgc cgctgtcgca gtaagcgaaa
    15301 tcacggaagt aatggtgggc gctcatttcg ccaccgtaga tggcgtcttc cttgcgcatc
    15361 cgttctttaa taaaggcgtg tccggttttc gacattaccg gcgtgccgcc tgcagcagtc
    15421 accacatcaa tagtgttcca agagagacgc ggatcatgga tgatcttcgc gccgggattt
    15481 ttttcgagga acgcttctgc cagcaggccg acaatgtagt agccctcgat aaactgccct
    15541 ttttcgtcaa acagaaaaca gcggtcaaaa tcgccatcaa aggcaatgcc catatccgcg
    15601 ccgtgtttga tgaccgcatt gcgggtgtcg tcgcgacatt ccggcagcag cgggttagga
    15661 ataccgttgg ggaaattgcc gtccggtgtg ttgtgtactt tgattaattc cacgggcgcg
    15721 ccgagggctt taaagcgggc ttcaatggcg tccaccaccg gacccgctgc gccattcccg
    15781 gagttgatca ccagcttgag cggcgtgaga tttttgacat tgatataacc gaacaggtga
    15841 tcaacgtaag cgtcacgcag gttgatttgc tgatagcgac cgcgtttggt ttcatcgacg
    15901 ggaggaaagt cgttggcttc agccagacgc tggacgtcgc gcagtccggt atcaccgctg
    15961 atcgtgcgag ccccctcgcg caccagcttc atgccgttgt aatccatcgg attatggctg
    16021 gcagtaactt caatgccgcc atccacgccg agatggaacg tggcgaaata gatctcttcg
    16081 gtgccggaca taccgatatc cagcacatcg acgcccgcat cctgtaaacc ttttgccagc
    16141 gccagtttta aggtttcgct ggtgagacgg acatcaccgc ctaacacaat ggttttcggt
    16201 ttgagaaatt cgccataggc gcgaccaatg cgccaggcga tatcttcatt cagttcttcg
    16261 cctaatttcc cgcgaatatc ataggcttta aagcaggtta attttttcat gtcgttaccc
    16321 tttttcaggc aatagttggc cctgaccgaa acggccaatt ttatttttgt cgttatttac
    16381 accgcggttt cgcattcatt gcctgatgcg acgctgccgc gtcttatcag gcctacaaaa
    16441 ccgaaccgta ggtcggataa ggcgttcacg ccgcatccga caaacagcgc ctgatgcgac
    16501 gtttacaccc gtccgtagcg atccgcgaag cgcaccacat catcctcttc gagataagag
    16561 ccggagcgca cttcaattaa atcgagcgga attttccccg ggttttccag gcagtgcgtc
    16621 gcccccagcg gaatataaat ggactcgttt tcaccaagca gtttgatatc accatcaatg
    16681 gtgacttttg ccgttcccgc gaccactacc cagtgttccg cgcggtgatg gtgcatctgt
    16741 accgacaaac cctcgcccgg tttcacggtg atgcgtttca cctggtagcg gtcgcccgcg
    16801 tcgatagagt catatttgcc ccacggacga tacacttcgc gatgcacccg atgctcatgg
    16861 cgaccatcgg ctttgatctg ctcgaccact tttttcacat cctgtaccgc gttacggtcg
    16921 gcaatcagca ccgcatcttt ggtctgcacc actaccagat ctttcacccc gacggtggtg
    16981 accaggcctg attcggcata cacatagctg ttttcagttt tgtgattaat cacatcgccg
    17041 tggcaaacgt tgccctcggc ggtgtgggcg ctgatctccc ataatgaaga ccaggaacca
    17101 acatcgctcc agcccgcatc catcggcacc acaacagcat ctgccgtagg ttccatgacc
    17161 gcgtaatcca ccgactcttc cgggcaggcg agaaacgctt cttcatccac acgaataaaa
    17221 tcgagatccg gatcgacggc gctcatcgct ttttcgcagg catcgaggat atccgggcga
    17281 tattttttca gttcttcgag atagcgtccg gcgcggaaca ggaacatgcc gctgttccag
    17341 taatattcgc cgcttgctac ataggcctgg gcggtttcca gattcggttt ttcgacaaac
    17401 tgcgccactt caaaggccac cgtatcctgc tcacccacag acacttcacc gcgacgaata
    17461 tagccataac cggtttctgg tagatccggc acaatgccaa aggtcaccag cttgcccgct
    17521 tcggcataag gcatggcatt acgcacggcg gcacggaacg cgtcttcatc ggcaatcaca
    17581 tgatccgccg ccaataccag cattaacggg tcgctctccg ggctatgacg ttttgccgcc
    17641 agcgccgcca gtgcaatggc aggtgcagtg ttacgccctg ccggttcgag aataatgttc
    17701 tcggtgagtt tgttcagttg acgcagctgt tccgcgacaa taaagcggtg ctgctcattg
    17761 caaatcacca ccgggctttc gcactccacg ccgttcaggc ggcagatggt ggtttgcagc
    17821 atggtgagat cgcctttcag gcataaaaac tgcttggggt aaagtacgcg ggaaagcggc
    17881 cataaacggc taccggagcc acctgccatc acaactggat agagtttcga ctgcgccata
    17941 attatccccg aatatcattt ataaattgac gtaacacgtt ctctttatcg agcgtgcgtt
    18001 cggcatattc acgtgccacc gtgttatgtt tgggcagcag gagcgcctga cgaatccccg
    18061 ccaccagcgc ctcgaccgat tccggttcta cgcaaaccgc aatacccgga aaggtttcgc
    18121 aaagctgccc cagttctgtg tgggcctctg cggtaatcac cgcgttaccg cctaccgcca
    18181 gaatattggt cagtttcgac ggcaatacgg catctgccgc gccgcgtttt tgcaccacca
    18241 gatggcaatc gcccatcttc agcagtgcag gtaaagcgtc atacgattgc agcgggaaaa
    18301 attgcatgtt gcgcagtcca cgctgctgcg ccattttttc cagccgcgct ttgccgccgc
    18361 tctgcccgac aatggcaaaa atcagcggtt catcgcgcag gagatcggca gcttcaataa
    18421 cgttttccag cccctgcttt tcaccaatat tgccggagta aagaatgatt tttttgttat
    18481 ccggcaggcc aagctggtta cgaagggcat caacatcggc gtctgcaaca tgctgaaaac
    18541 gggcaatttc cgaccagttg gggaagaaga tgacgttatc cgccgccacg cctttttcga
    18601 tggctttatt catcatcgaa cgcgaaatcg tggaaacgtt atcgacgtta tgcagtccgc
    18661 tacgttcgaa cgccgttgcc agctgtgcca ctttgccgcc tttgcctttt ccggcaaggc
    18721 ccagccccag catggcgtcc acttcgtaat cctgaatatg cagcacggta cgcgcaccag
    18781 agagcttcgc cagcaggcgc attcccggcg tgcaaaacag cgttggcact acgccaataa
    18841 tgcgatccgg cttccagcga cgttgcgcca tcagtggaaa gaaactgctg acggcaaaac
    18901 tgccgagatg caacaagcgt ttcagggtgc tcggctgttt tggcacgtac agcgggcagc
    18961 gccacaccgt ggcagcccct tcttctcgtt tgtagcgcca ggcggaatag ttctcaccca
    19021 cctgccattg cgggtagtaa ggcggtgcgg taatgacccg cacctcatga ccttgtgccg
    19081 ccaaccattc caccatctcg ccggtgtatt tgccgatgcc ggttaactcc ggcgagtagt
    19141 taatgccgta gaccagtatt ttcataatcc gggtactccg gcacgcttct cagcgagaaa
    19201 ataggctcgg ctgttagcgt gaacatcgtt actggcgagc agcgcgtccg gcgtcagcca
    19261 gcggtaatcg tcatgctgct catccggcag taacagctct tcttccgcta ctctgaagcg
    19321 aaaaccgagc accacatagt gagtggtgaa atccgtgccg gagaagttat cgtcataaaa
    19381 atgctgccag acaccgtaaa actggcctgc tgttatcggc agacgcagcc ccagttccgc
    19441 catcgtcagc cgctcaaatg cggcttccag cgtttcgtct ttctgcacgc gccctcccgg
    19501 cacaaaccag taaccctgcg ccgggcggtt ggttcttttg ccaagcagaa actcgccgcg
    19561 actgttctcg acaataaagt cgagagagac aagcggtgtg gagcgcacta cagtggcaaa
    19621 gtcttcctga cgtaaaaaca tcattccccc cgaaagcggt cttgattctc aaggaaccac
    19681 tggtaagtgc tggcaagccc cgcttccagt gagatttcgt gataccagcc aagctgatgc
    19741 aggcgcgtca catccagcag tttgcgcggc gtaccatccg gtttgctggc atcaaaaacc
    19801 acccgacctt tgtaacccac cactttggcg atggtttgcg ccagctcgcg gatagtgcag
    19861 tcaacgcccg tgccgacgtt aatgtgcgac agcatcggtt gggtattctc cagccagact
    19921 tcatgcgcca gctccatgac atgaatgctc gccgccgcca tatcatcgac gtgcaggaat
    19981 tcacgcatcg gtgtaccgct gccccatacc accacgtccg tgccgtcgcc tcgtggaagc
    20041 gacgcagcaa tgctggtatc acatgggagt tactcgggtg gaagttgtca tgcggcccgt
    20101 acaggttggt cggcatgaca gtgcggtaat cgcgtccata ctggcggttg taagattcgc
    20161 acagtttgat cccggcgatt ttggcaatgg cgtaaggctc gttggtcggc tccagcgtgc
    20221 cctgcaataa ctcgctttct gccatcggct gttttgccag tttcgggtag atgcaggacg
    20281 atccgagaaa catcagtttg ttcacgtcgt tctgatgcgc ggcgtgaatg atgttgctct
    20341 caatcatcat gttctggtag atgaaatccg ccggataggt gttgttggca acaatgccgc
    20401 ccactttcgc cgccgccaga tagacctggt caatgcgttc gctggcaaag aaatcatgca
    20461 cggcgcggct gtccagcagg ttcagttcat cgcgggtacg taataccagc tccacatcac
    20521 cgcgctgttc gagctgccgc ttgatggcag aaccgaccat cccacaatgg ccagcgataa
    20581 aaatgcgttg tttactcatg cttatgactc cagcgcgatc gccacgtcgt agccgtgaga
    20641 tttcagcaga gagtgttttt tcgccgcttc gaggtcatta gccaccattt cagacaccat
    20701 ctctctgagg gtgatttccg gtttccagcc cagtttttcg tgcgctttgg tcgggtcgcc
    20761 gagcagcgtt tcaacttctg ccggacggaa gtaacgcgga tcaacggcga taatcacatc
    20821 acccggttta acgcccggcg cgtcatgccc ggtgacggaa accacaatgc ccttctcttc
    20881 aacacccgtg ccttcaaagc gcagtttgat gcccagctgt gctgccgcca tttccacaaa
    20941 ctgacgtacg gagtactgaa cgccggtcgc gataacgaaa tcttccggct gttcctgttg
    21001 cagcatcatc cactgcattt ttacgtagtc tttggcgtgg ccccagtcac gcagggaatc
    21061 catattgccg aggtacaggc acgactccag cccctgggcg atgttggcga ttgcgcgggt
    21121 gattttgcgg gtaacgaagg tttcgccgcg gcgcggggat tcatggttga agagaattcc
    21181 gttacaagcg tacatgccgt aggattcgcg gtagttaacg gtgatccagt aggcgtacag
    21241 tttggcgacc gcatacggag atcgcgggta gaacggcgtg gtttctttct gcggaatttc
    21301 ctgcaccaga ccatacagtt cagaggtgga agcctgatag aaacgagttt tcttttccag
    21361 accgaggaag cggatcgcct ccagcaggcg cagcgtaccc atcgcgtcga cgtcagcggt
    21421 atattctggt gactcaaaag agaccgcaac gtggctcatt gcgcctaggt tgtacacttc
    21481 atccggctgt acttcacgca aaatgcgcgt caggttagag gtatcactca ggtcgccata
    21541 atgcagatgg aatttcgggt tgcaggtgtg cggatcctga taaatgtgat ccacgcgctc
    21601 ggtgttgaat gacgatgcgc gacgcttaat accatgcacc tcgtaacctt tttccagcag
    21661 aaactctgcc aggtaagaac cgtcttgtcc ggttacaccg gtgatgagag cgacttttga
    21721 catgtcttat tcctctgtat ttttgaattt attcagtttc aacgcgttcg cgtatcacca
    21781 ctgcgggatt cccccggcaa accacatttg ccggaagcga tttaaaaaca ctgcttcgtg
    21841 cacccacgac ggtgccgtcg ccgattgtga cgcctggggc aacaaagaca tcggttgcca
    21901 gccagcattt ctcgccaatc acaataggcg tggcgttaat ggtgaaatgt tgacttgcat
    21961 ggtcgtggct accggtgcat aaataacttt tttgcgatat caccgaatgt gcgccaatgg
    22021 ttatttcacc gagggtatat aaattgacgt catcgccaac ccacgcgtaa tcacctaagg
    22081 ttaatttcca cggataggta atttttactg acggacgaat aactacgttt tttcctattt
    22141 ttgctccgaa taaacgtaat aaaaaagccc gccagcgata caatacttgt ggcgaccagg
    22201 caaatattgt tgcctgtact gcccaccata attgcacttt aatagcgttg ccgccccgga
    22261 accctttcgg caccgagaaa ccgcttaaat cttgcattgg ttttccttat attcagactt
    22321 tgttatataa ggctttagtc ttacctgtcg tacgttggcg taaatgccag gataattctg
    22381 cccagaagcc aggtacatgt aatatctgcc gttggacttt tttggcgtca gcac
//
LOCUS       DAEEUE010000062        22160 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.062, whole genome shotgun sequence.
ACCESSION   DAEEUE010000062 DAEEUE010000000
VERSION     DAEEUE010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22160
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.062"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..408)
                     /gene="sxy"
                     /locus_tag="KLK84_002564"
     CDS             complement(<1..408)
                     /gene="sxy"
                     /locus_tag="KLK84_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HBE2009840.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEV"
     gene            627..1136
                     /gene="sulA"
                     /locus_tag="KLK84_002565"
     CDS             627..1136
                     /gene="sulA"
                     /locus_tag="KLK84_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HBE2009841.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            1492..2538
                     /gene="ompA"
                     /locus_tag="KLK84_002566"
     CDS             1492..2538
                     /gene="ompA"
                     /locus_tag="KLK84_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HBE2009842.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(2614..3066)
                     /gene="matP"
                     /locus_tag="KLK84_002567"
     CDS             complement(2614..3066)
                     /gene="matP"
                     /locus_tag="KLK84_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HBE2009843.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            3251..5011
                     /locus_tag="KLK84_002568"
     CDS             3251..5011
                     /locus_tag="KLK84_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HBE2009844.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            5080..5598
                     /gene="fabA"
                     /locus_tag="KLK84_002569"
     CDS             5080..5598
                     /gene="fabA"
                     /locus_tag="KLK84_002569"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HBE2009845.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(5668..5835)
                     /gene="rmf"
                     /locus_tag="KLK84_002570"
     CDS             complement(5668..5835)
                     /gene="rmf"
                     /locus_tag="KLK84_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HBE2009846.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(6091..6654)
                     /gene="pqiC"
                     /locus_tag="KLK84_002571"
     CDS             complement(6091..6654)
                     /gene="pqiC"
                     /locus_tag="KLK84_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HBE2009847.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            complement(6651..8291)
                     /gene="pqiB"
                     /locus_tag="KLK84_002572"
     CDS             complement(6651..8291)
                     /gene="pqiB"
                     /locus_tag="KLK84_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HBE2009848.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            complement(8296..9549)
                     /gene="pqiA"
                     /locus_tag="KLK84_002573"
     CDS             complement(8296..9549)
                     /gene="pqiA"
                     /locus_tag="KLK84_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HBE2009849.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            complement(9679..11586)
                     /locus_tag="KLK84_002574"
     CDS             complement(9679..11586)
                     /locus_tag="KLK84_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE2009850.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            complement(11598..13706)
                     /gene="rlmKL"
                     /locus_tag="KLK84_002575"
     CDS             complement(11598..13706)
                     /gene="rlmKL"
                     /locus_tag="KLK84_002575"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HBE2009851.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            13950..15059
                     /gene="ycbX"
                     /locus_tag="KLK84_002576"
     CDS             13950..15059
                     /gene="ycbX"
                     /locus_tag="KLK84_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HBE2009852.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRTNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            complement(15056..15598)
                     /gene="zapC"
                     /locus_tag="KLK84_002577"
     CDS             complement(15056..15598)
                     /gene="zapC"
                     /locus_tag="KLK84_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HBE2009853.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMLLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(15772..16782)
                     /gene="pyrD"
                     /locus_tag="KLK84_002578"
     CDS             complement(15772..16782)
                     /gene="pyrD"
                     /locus_tag="KLK84_002578"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HBE2009854.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(16893..17633)
                     /locus_tag="KLK84_002579"
     CDS             complement(16893..17633)
                     /locus_tag="KLK84_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HBE2009855.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGGEASTVPALLLAAPPLILLKPG
                     TTGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLP
                     GDPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWC
                     QVIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            complement(17599..18114)
                     /locus_tag="KLK84_002580"
     CDS             complement(17599..18114)
                     /locus_tag="KLK84_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBE2009856.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(18122..18664)
                     /locus_tag="KLK84_002581"
     CDS             complement(18122..18664)
                     /locus_tag="KLK84_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE2009857.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGYFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(18762..19745)
                     /locus_tag="KLK84_002582"
     CDS             complement(18762..19745)
                     /locus_tag="KLK84_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE2009858.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGLRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIRE"
     gene            complement(19736..22108)
                     /locus_tag="KLK84_002583"
                     /pseudo
     CDS             complement(19736..22108)
                     /locus_tag="KLK84_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
BASE COUNT         5495 a         5804 c         5204 g         5657 t
ORIGIN      
        1 cacctccccg agaatcgctt ccagatgaaa agacatattg ggcaaatctt tcagtctttc
       61 ccgggtattg cgcgtgcttt tctctttcag cgctgcatcg agagaatatt tcgacagacg
      121 caccagcttc agttgatttc gccatagact ttcatcaacc cgatagtaat tgagggtaac
      181 ggatcggcca cactttttat atgtcagcca gacaggcgga tgttttacac agtactgtgc
      241 actttgctca caagcccgaa gatacaactc accatcagaa accatcgcaa acaccgtgtc
      301 gtcaacggtc aggctgtaac tgccaaacaa tgatcggtat tcaattgtgc ccaacgttgc
      361 caggtattct tgtgatttat agatccgctt ataggagagg cttttcataa aattcctttt
      421 aaaatcataa cataaaagaa tgattcacat taacggatcc gttaactacg aaaataggca
      481 acttattctt aaggggcaag attaatttat gttttcccgt caccaacgac aaaatttgcg
      541 aggctctttc cgaaaatagg gttgatcttt gttgtcactg gatgtactgc acatccatac
      601 agtaactcac aggggctgga ttgattatgt acacttcagg ctatgcacat cgttcttcgt
      661 cgttctcatc cgcagcaagt aaaattgcgc gtgtctctac ggaaaacact acagccgggc
      721 ttatcagtga agttgtctat cgcgaagatc agcccatgat gacgcaactt ctactgttgc
      781 cattgttaca gcaactcggt cagcaatcgc gctggcaact ctggttaaca ccgcaacaaa
      841 aactgagtcg ggaatgggtt caggcatctg ggctaccctt aacgaaagta atgcagatta
      901 gccagctctc cccttgccac acagtggagt caatggttcg cgctttacgc acgggcaatt
      961 acagtgtggt gatcggttgg ttgacagatg atttgactga agaagagcat gctgaacttg
     1021 ttgatgcggc aaatgaaggt aacgctatgg ggtttattat gcgtccggta agcgcatcct
     1081 ctcacacaac gagacaactt tccgggctaa aaattcactc taatttgtat cattaagtaa
     1141 atttaggatt aatcctagaa ctttttttgt cgcccagcca atgctttcag tcgtgactaa
     1201 ttttccttgc gaaggcttgt ctgaagcggt ttccgcgttt tttttctgta aattgtcgct
     1261 gacaaaaaga ttaaacatac cttatacaag actttttttt catatgcctg acggagttca
     1321 cacttgtaag ttttcaacta cgttgtagac tttacatcgc caggggtgct cggcataagc
     1381 cgaagatatc ggtagagtta atattgagca gatcccccgg tgaaggattt aaccgtgtta
     1441 tctcgttgga gatattcatg gcgtattttg gatgataacg aggcgcaaaa aatgaaaaag
     1501 acagctatcg cgattgcagt ggcactggct ggtttcgcta ccgtagcgca ggccgctccg
     1561 aaagataaca cctggtacac tggtgctaaa ctgggctggt cccagtacca tgacactggt
     1621 tttattccta acaatggtcc gacccacgaa aaccaactgg gtgcaggtgc ttttggtggt
     1681 taccaggtta acccgtatgt tggttttgaa atgggttacg actggttagg tcgtatgccg
     1741 tacaaaggcg acaacatcaa cggcgcatac aaagctcagg gcgttcagct gaccgctaaa
     1801 ctgggttacc caatcactga cgatctggac atctacactc gtctgggtgg tatggtatgg
     1861 cgtgcagaca ccaaggctaa cgtacctggt ggcgcatcct ttaaagacca cgacactggc
     1921 gtttctccgg ttttcgcagg tggtgttgag tacgcgatca ctcctgaaat cgctacccgt
     1981 ctggaatacc agtggaccaa caacatcggt gacgcaaaca ccatcggtac tcgtccggac
     2041 aacggtctgc tgagcctggg tgtttcctac cgtttcggtc agggcgaagc agctccggta
     2101 gttgctccag ctccggcacc ggaagtacag accaagcact tcactctgaa gtctgacgtt
     2161 ctgttcaact tcaacaaagc aaccctgaaa ccggaaggtc aggctgctct ggatcagctg
     2221 tacagccagc tgagcaacct agatccgaaa gacggttccg tagttgttct gggttacact
     2281 gaccgcatcg gttctgacgc ttacaaccag ggtctgtccg agcgccgtgc tcagtctgtt
     2341 gttgattacc tgatctccaa aggtatcccg gcagacaaga tctccgcacg tggtatgggc
     2401 gaatccaacc cggttactgg caacacctgt gacaacgtga aacagcgtgc tgcactgatc
     2461 gactgcttgg ctccggatcg tcgcgtagag atcgaagtta aaggtatcaa agacgttgta
     2521 actcagccgc aggcttaagt tatcgtctgg tagaaaaacc ccgctgctgc ggggtttttt
     2581 ttgcctttag taaattgaac tgactttcgt cagttattcc ttacccagca atgcctgcag
     2641 atcctgctta agagaagaca ttttattcgc gtatttctct ttgttttccg catcttcaat
     2701 cagctgaaca atcgtttcag aaagcgtttt accgcgacgc tgtgcaagac cagccagacg
     2761 ttgccagaca ataaattcca gatcgatcga ttttttgcgc gtatgctggt gttccgcatt
     2821 aaaatggcgc ttccgtctgg cccgaatggt ctgcttcatg cgattgacca gttccggatt
     2881 catatgcttg tcaatccagc catttaccag cacgggttca ttttcgagcg agagcaacac
     2941 atcgacggct tcctgggcgg cactggcttc tatgtaacgg gtaattaact ccccttcgcg
     3001 atgcttcttc accagatact tccatttcca accgctttca agattttcaa gttgttgata
     3061 tttcatagcg atctcaatgt taccgtgtaa ctctttacag aatatcagct ttttaggcca
     3121 gtgaagaaaa gaatctccat cctgtgaact ttaacgccca gccaggagta ttgtcggtgc
     3181 tttctacgtg tgctgtagtg ccggttacgg tataatcgcg gctttgacaa cagactaaaa
     3241 aacatcaact ttgaccatta cgaaacttgc atggcgtgac ctggttcctg ataccgatag
     3301 ctatcaggaa atatttgctc agccacattt gattgacgaa aacgatcctt tattcagtga
     3361 tactcaaccg cgactgcaat ttgcgctgga gcagttgctg catacgcgag catcctcctc
     3421 ttttatgctg gctaaggccc cggaagagtc tgagtatctg aatcttattg ccgatgccgc
     3481 gcgtacgcta caaagcgatg caggccaact ggtgggcgat cactatgagg tttccggcca
     3541 ctccatccgc ttacgtcacg cagtgagtgc agatgataat tttgcgactt taacgcaagt
     3601 tgtcgctgcc gactgggtag aagcggagca actctttggc tgcctgcgcc agtttaatgg
     3661 cgacattacc ctgcagcctg gtctggtgca tcaggcaaat ggcggtattc tcatcatctc
     3721 tttgcgtaca ctgctggcgc aacctctgct gtggatgcgg ctgaaaaata tcgttaaccg
     3781 cgagcgtttt gactgggttg cgtttgatga gtcgcgccct ctccccgtct ctgtgccttc
     3841 gatgccattg aagctgaaag tcattctggt aggcgaacgc gaatcattgg ctgatttcca
     3901 ggagatggag ccagagcttt cagagcaggc tatttatagc gaatttgaag atactctgca
     3961 gattgtcgat gcggagtcag taagccagtg gtgtcgctgg gtgacattta ccgccagaca
     4021 taatcactta cctgccccgg gagcggatgc ttggccggta cttatccgcg aagcagcacg
     4081 ctacaccggt gaacaagaaa cacttccgct tagcccgcag tggatcctcc gccagtgtaa
     4141 agaggtcgcc tccctgtgtg atggcgacac cttctccggc gagcagctaa acttaatgct
     4201 gcagcagcgt gaatggcgcg aaggtttcct cgctgaacgt atgcaggatg agatccttca
     4261 ggagcaaatc ctgattgaaa ccgaaggcga acgcatcggg caaattaacg ccctttcggt
     4321 cattgaattt ccgggtcatc cacgcgcttt tggcgaacct tctcgcatta gctgcgttgt
     4381 gcatattggc gatggtgaat tcaccgacat cgaacgcaaa gcggagcttg gcggcaatat
     4441 ccatgcgaaa gggatgatga tcatgcaggc gttcctgatg tcggaactac agcttgagca
     4501 acagatcccc ttctcagcat cgctgacatt tgagcagtca tacagtgaag tggatggcga
     4561 tagtgcctcg atggctgaac tctgcgccct gataagcgcc ctcgctgatg tgccagtgaa
     4621 tcagagtatc gctatcacag gttcagtcga tcagttcggc cgcgtccagc cggtcggtgg
     4681 tttaaatgag aaaatcgaag gcttctttgc tatttgccag caacgtgagt taacggggaa
     4741 acaaggtgtc attatcccca ctgctaacgt tcgccattta agtcttcaca gtgaactggt
     4801 gaaagcggta gaagaaggca aattcaccat ctgggcagta gacgatgtga ctgacgcact
     4861 gccgttatta ttaaatctgg tgtgggatgg cgaaggccaa acgacgctga tgcaaaccat
     4921 ccaggaacgt atcgcgcaag catcgcaaca ggaaggacgt caccgttttc catggccatt
     4981 acgttggctg aactggttta ttccgaactg atcggacttg ttcagcgtac acgtgttagc
     5041 tatcctgcgt gcttcaataa aataaggctt acagagaaca tggtagataa acgcgaatcc
     5101 tatacaaaag aagaccttct tgcctctggt cacggtgaac tgtttggcgc taaaggcccg
     5161 caattgccag caccgaacat gctgatgata gaccgtgtgg tcaaaatgac cgaaacgggt
     5221 ggtaacttcg acaaagggta tgttgaagca gaactggata tcaatccgga tctgtggttc
     5281 ttcggatgcc acttcattgg cgatccggtt atgccgggat gcctgggcct ggacgcaatg
     5341 tggcagctgg tagggttcta cctcggctgg ctgggcggcg aaggtaaagg ccgcgcgctg
     5401 ggcgttggcg aagtgaaatt cactggtcag gtactgccga cagcgaaaaa agtgacctac
     5461 cgtattcact ttaaacgcat tgttaatcgt cgtctgatta tgggtctggc agatggcgaa
     5521 gtgctggttg attgtcgtct gatctatacc gccagcgacc tgaaagtcgg tctgttccag
     5581 gatacgtctg ccttctgatt atcatcagac agagtatcaa aaggcgaaac ctccgcaatg
     5641 cggaggtttc tttttaaaga gacagaatca ggccattact accctgtccg ccatggcttc
     5701 tcgccagcct cccagccatt gtgacctttg actcagcgtc tgatagggac acatttcttt
     5761 tgagcgtccg gcgatgccgg cctgataacc acgttgatgt gcccgttcca ggcgatctcg
     5821 tttttgtctc ttcatgcctc gtttccctca tactggtttc tggtggaaaa gaaaacagtg
     5881 attactaaat gtgcaatcac agttaagcaa taccgcgaat cataactgcc gtcaatgcgc
     5941 aaaattcacg ccactgtcat atttgtgagc tacacgggca tgtcggttgt acaaaaaatg
     6001 tgaagaggaa caaatttttc cgcacgttag aaacaaaaaa accgcctcag gcaacatacc
     6061 ggagacggtt atatttacaa atctttataa ttaaggtaga cgctttatct cttgtgcaat
     6121 agaagcggct tcctgactcc agacaccggc cagcacttta accatctcat cgtaaccatc
     6181 ctgagtttgc actccttcca gacggaacgg acgtttgatc agttgtccct ggtggttcaa
     6241 cagccactca ccactgacaa tgaccttgcc atcatagcga ccgttaaact ccgttacggt
     6301 aacattgagc gtgtcctggg cgcttcccag aggctgggag gcaaccaccc agccgggcag
     6361 ttgcgtactc aggttggcaa ccagggtgtt gcgcaactgt tgatccaacg ggctggccca
     6421 caagttgttg ttggcaatca catacttcac atcactggtt tgataaacca caccattccc
     6481 cgccagatag tcaggaacag tgacctgctc tacccataac agacgattgc cctggctggc
     6541 ggtactttgt gtaccgctct gcaccacagg taactggtaa tagtttttat taatttcgcc
     6601 ggagctgcat ccggccagcc acagtgctgc aatcgtcact agccactttt tcattgtttc
     6661 gccctcttcg gctctggatc ttttttgtcc ttcgcttcaa ataccagcgc gttactcttc
     6721 tcattgagcg ttttcagcac cggttgcagt tctcgcaaca cctgatcaag gcgctgcata
     6781 tccgccacca tcttgttgta ggctgcggag ccaggctgga agccctgcat gctgcgattc
     6841 aattcacgca acgttgactg catatccgtc ggcaactgct gcatcgactg gctggcgagg
     6901 atcttgttca tgctatccag cgtcgtttgc aggtttttca ttgtgcgctg actttcagaa
     6961 agcgtactgg ttgcctgttc aatcatcgga ttcaatggca gtttgttgat cttatccaac
     7021 gcttccatca gtcgttgctg gatttgcgcc aagccgccgc taacagtcgg gataatctga
     7081 taaccattaa attcacgaat accggttatt gcaggcgtat ttggatagaa atcgagatca
     7141 acatacagtg caccagtgac caggtttccg gttttcagcg atccgcgtaa accacgtttc
     7201 aacaattcgc caaggtgctc aacaacatcc gcattttcgc caagctgcat tttcagccgc
     7261 tctggctcga tacgaatcag taccggaata cggtaatcat cgttaaatgt ctgacgcata
     7321 ttcggcgcaa agaatggcac tttgcttacg gtacccaggc gaataccccg gaattctacc
     7381 ggagctcccg gttgcagacc gcgtaccgaa tctttaaaga acatcagata atcaatgtga
     7441 tcggtgtaca acgaatcctg aatgctcttc tgatcatcat acaaaacgaa agctgttttc
     7501 ggtgccactg gctgccctaa atccagacct tccggcacat caaagctaac accgccacta
     7561 agcagcgttg tcaatgagcc catctccaca cgcatccccg ctgacgtcag atcaaccgca
     7621 atgccactat ctttccagaa gcgaacattg ctggtcacca gtcggtcata aggtgcattg
     7681 atgaacagtt gataactgat attgcgtttt tgcgtatcga aggtgctggt ttcaaccgaa
     7741 cctacccgat agccacggaa cagcaccgga tctcctggcg agagctgccc ggcttttttg
     7801 ctatcgagaa tcacacggat acctttcgca tcaggcgggg ccaacggtgg tgagtccagc
     7861 aaatcgtatt tatccatttt gctgcctttc gcgcctggct gcagttcgat ataaactcca
     7921 gacagcagcg ttcccaggcc gctaatccct tcgcgaccaa tctgcggttt caccacccaa
     7981 aagacggtgt ctttatgcag caatttttcc ataccggaat tcagccgcgc tttgatttca
     8041 acgtgcgtca aatcatcagc cagtgtggcg ctttcaacca cgccgacgtc aacgctacgg
     8101 cttttaatgg tggttttgcc accttcaatt ccttccgcat tcgcggtgat cagggtcact
     8161 tccggtccct gatggctgta atgataaaaa agaacccagg ccccaatgag cgccgtgacg
     8221 ataggaaata tccacacggg agaccagttc ttcactttct ggattttggc ttccccatta
     8281 ttagattcca tactgtcagg actcctcatg ctctgattca ggttgacgat cccacgacaa
     8341 acgcgggtca aacgtcatag cagaaaacat tgtcattatg acgactaaag caaacattaa
     8401 tgcacccatt gccggataaa tactcattaa acctcccata cgcaccagcg ccgagagcac
     8461 cgcgataacg aaaacgtcaa tcatcgacca gcggcctaca aactcaacaa cttcataaat
     8521 caaatgcatt ctttcactgt cgcgcttgcc atgccctttg gcatcccaac acagccacgc
     8581 gatggcgatc atctttaacg ttggcaccat aatactggcc agaaagatca ccgcagcgac
     8641 gggataagag ccttcgctcc ataacagaat gaccccagcg agaatcgtcg acggcatctt
     8701 cgagcctaat aaatccgtca ccatgatggg caaaatatta gccggaagat acagcatgat
     8761 ggacgttaca agcagcgcga gtgtccactg caggctgttt ctacgccgaa cgtacccttt
     8821 ggtactacaa cgcgggcaca cgggttcatc agcaggaagg attgccgtac aacaggagca
     8881 cgaacgcagc ccctgacgta tccccgtgac gcctggtttt agcggctggc gcagttctgg
     8941 catcggggcg atgtcgtccc ataaccagcg acgatcaacg cactgaaaag cgcgcagttg
     9001 caggacacaa aataaacacc aggggagaaa gctgctgcct accccaatgc tgccgtaagc
     9061 catcagttta acgaaactga ccagcacacc cgcgaggaaa atctccgcca ttccccaggt
     9121 tttgagttga aaaagcaccc gtgccagttg ctcttttaaa cggaccggta attccgcgcg
     9181 attcaccagt aacagaatgg ttatcagaca aaacgcggga accagttgca caaacaacag
     9241 gaaaaaggtg ccgaggctgg cgtagtcctc agaaaaaagc acgccgggaa tttccagtaa
     9301 tgtaatttca ctggtaactc ctgcaacgtt catattcaca aaaggaaaca agttggacag
     9361 caacagcatg aacagtgcag ccaacgcata ggcggtggga cgctgccgag gggcatccca
     9421 cgccacggtt aacgttgtgc cacaccgggg acatgccgct ttctgaccat gctcaaggcg
     9481 cggtaacgcc accagcatgt cacactgcga gcacaggatg tgcttcgcgg catgatgatg
     9541 ttcgcacatt ggtactcctt aatgatgcgc tattttacag atgactaaca aagtgttggt
     9601 tttcatgcct gataaaccca gttcatcagg caattttacc gttgtcagct gtctctgttt
     9661 aaatcgacta ttttgcgatc agccaccatt ttttaacgct tcaagatact cccagcgttc
     9721 aaaggcttgc tccagctcct gctctgcagc agccatatca gcaagcactt tttgcgtctg
     9781 ctcatgcggc tgactgaaga aggaagcatc cgccacttgc gtctgtaggg cttccagctt
     9841 cgcctccaga tcttcgagca attgcggtag ctgctccagt tcgcgctgca atttatagct
     9901 tagtttgcta ctgctgcgtt ttacagtttc tgcttttggc gcggcggctt cttcgctttt
     9961 tttcaccgca ggctgtttga gtgccacata ttgttcttgc tgaccacggg catcatgata
    10021 accgccaaca taacgaccaa ttttaccgcc gccttcgaag atccagcatt ctgtaacggt
    10081 gttatcgaca aactgacgat cgtggctaac cagcaatacc gtgccctgat agctgtcgat
    10141 cagttcttcc agcagttcca gcgtttcgac atcaagatcg ttggtcggtt cgtcaagaat
    10201 caataagttg cttggtttga ggaacaaacg cgccagcagc aagcggttcc gctcaccgcc
    10261 agaaaacgca cgtactggcg tcatcgcccg tttcggatgg aacagaaagt cctgcaaata
    10321 gcccaatacg tggcgtggct tgccattaac catcacctct tgcttacctt cggcaaggtt
    10381 atccatcacc gttttatcgg gatccagttc cgcgcggtgc tgatcgaaat aagccacttc
    10441 cagtttggtg ccaacgtgaa tacgcccgct gtccgcttga agctgaccga gcatcagttt
    10501 cagcagcgtg gttttgccgc acccattcgg accaatcagg gcaattttgt cgccacgtag
    10561 aacctgggca gaaaaatctt tcaccagttg cttaccgtta acctggtagc aaacgtcttc
    10621 catttcgaaa acgatcttac cggagcggct ggcctcttcc acctgcatct ttgcggtacc
    10681 catcacttcg cgacgttcac cacgttcgcg acgcatcgct ttcagggcgc gtacgcggcc
    10741 ttcattacgg gtacggcgtg ctttgatccc ctggcgaatc cacacttctt cctgcgccag
    10801 tttgagatcg aactcggcat tttgtagttc ttccacgcgc agggcttctt ctttttcctg
    10861 caggtactgg tcgtaattcc ctggataggt caccagcttg ccgcgatcga gatcaacaat
    10921 gcgcgtcgcc atattgcgga taaacgaacg gtcgtgggag atgaaaataa tcgtcccatt
    10981 gaaggtttta aggaacccct ccagccagtc gatggtttcg atatccagat ggttagtcgg
    11041 ttcgtccagc agcaatacgc gcggattgct caccagcgcg cgtcctaatg ccgctttacg
    11101 caaccagccg ccggaaagcg acgacagcgc aacgttagga tctaacccca gttgcgccag
    11161 cacttcgttg atgcggtttt ccagctgcca caggttgtgg tgatccagct gttcctgaac
    11221 cttcgccagt tcgttgagat ttttctcgct cgggtcgttc atcaccaggc gcgaaatatc
    11281 gtgatagcgt ttcagatatt ccgcttgttc ttcaatgcct tcggcaacga aatcataaac
    11341 gctaccctca acgttacgcg gtggatcctg ttgcagacga gctacaatca aatcttgctc
    11401 ataaataatg cgaccgtcat ccagcccttg ttcacggttg aggattttca ttaacgtcga
    11461 ttttcctgcg ccgttgcggc ccaccagaca aacacgttcg ttatcttcga tatgcagttc
    11521 tgcgttatcg agaagcggcg cgtcgctgaa cgacagccat gcgccatgca tactgattaa
    11581 tgacattact attcctttca ggctgcggta atcagccagc agttgtggat ctggcggtta
    11641 cgggcgaaat cctgggagag cgttttttgc gtaatttctt gtgctttcag tcccagtttt
    11701 gccaggccgt cgagatccat acggaagcca cgtttgttgt tcgagaacat gatcgtccca
    11761 cctgcacgca gcagacgttt caaatctttc atcagcgcca gatgatcgcg ctgaacatca
    11821 aacgcatctt ccattcgttt tgagttagag aaggttggcg gatcgataaa gatcagatcg
    11881 aactgttcat ttgcctcacg cagccacgcc aggcaatcgg cctgaatcag gcgatgcgca
    11941 cgcctagtca ggccattcag acgcaggttg cgttctgccc attccagata agtacgcgac
    12001 atatccacgg tggtggtgct gcgtgcaccg cctaatcccg catgcacggt ggcgctgccg
    12061 gtataagaaa acaggttgag gaaatctttg cctttgctca tctgaccgag catacggcgg
    12121 gcgatacggt gatcgaggaa cagaccagta tcgagataat ccgtcaggtt cacccacaag
    12181 tgagcgttat attcggtaac ttcaagaaac tcgcccttct cgcccagttt ctggtactgg
    12241 tttttgccct tatggcgttc acgggttttc agcaccagtt tgtttggcgc aatccccagt
    12301 accgaaatgg ttgcagcgat aatatcgaac agacgctgac gcgctttgtg cgcatcaata
    12361 gtttttggcg gcgcatactc ctgcaccacc acccagtcgg cataacggtc aacggcaacg
    12421 ttatattccg gcagatcggc gtcatacagg cggtaacatt caatcccttc ctggcgtgcc
    12481 cacttctcga attttttgag gttcttacgc aggcggttgg cgtaatcttc cgctaccatt
    12541 gccggtttac tgtctggtgt gctttcggca acgtggtaat ttttctgtac gcagtccagc
    12601 gggccgtttt tcgccttgta ctgtttgtct gcacgcagct gcaggcagct tagcagatcc
    12661 ggcgaggcac taaacaaaga gagattccag ccaccgaact ggtttttcat gattcggccc
    12721 agcagactat gcagcgcaat cagcgccggt tcgctgtcca gacgttcacc gtatggcggg
    12781 ttgctcaaca ctgtaccgta cggccctttc ggcagcggat tggtcagttg cgcgacatct
    12841 ttcacctcaa aggtgatcag ttcaccaatc cccgcaagac gggcgttagt gcgcgcacgt
    12901 tgaatcaccc gtgcgtcgct gtccgaaccg taaaaatgag agctatactc agccaggcct
    12961 ttacgggcgc gagtttgcgc ttccgctttc acttcctgcc agatagcttc atcatgctgc
    13021 gcccagccgc taaagcccca acgcccacgg tgcaagcctg gtgcgcgatc ggtcgccagc
    13081 atcgccgctt caatcagcaa cgtaccggaa ccacacatcg gatcgagcag cggtgttcct
    13141 gactgccagc cggatcgcat cacaatagcg gctgccaggg tttctttgat cggcgcaata
    13201 ccagcacgat cgcgatagcc acgcagatgt aaaccatcac cactcagatc aagagcgata
    13261 ctggcggttt ctttatgcag ccagacgtta acgcggatat ccggcgcatc gcgatcaaca
    13321 tttggacgcg gcagattttt ccgcgtgaaa gcatcgacga tcgcgtcttt cactttcatc
    13381 gcgccgtact gactgttgcg gatggtgtca ttcaaaccac tgaagtggac agcgaaggtc
    13441 gcgccaggat taaacatctc tgtccagttg atcgcctgaa caccgagata gaggtctaaa
    13501 tcgctgtaaa ccttacactc gcccagcggc aacataatac gcgaggccag gcggctccac
    13561 atcaggctct ggtaaacaag ccgtgtgtcg cccttgaaat ggaccccacc ctgaaccacc
    13621 tggcattcaa cggcccccag attttccagt tcagttttta acagctcttc cagcccacgg
    13681 gccgtactgg caaacagaga attcatatcg tcacttttac tctaagagcc taccgcagta
    13741 ggcgtaattg ttgcagtcag tctggacatg gagagcgcgg agaaaccgga gcgtacacaa
    13801 gtacgtgagg atttcgagca caacccaggg ccaaactgac aaataaaata gcctaacggg
    13861 acaggcgcta agaaaattgt tgcgcattat agctaataag cgcgtcatgt cataaagttg
    13921 agggcttatt ttcatttgag gaccgcaccg tggcgacatt aacccggctt tttattcatc
    13981 ctgttaaatc gatgcgcggc attggtctta cacatgctct ggctgatgtc agtggtctgg
    14041 ccttcgatcg catctttatg atcacggaac ctgacggtac gtttattacc gctcgccagt
    14101 ttccccagat ggtacggttt actccttcac ctgtgcatga tggcttgcat ctcaccgcac
    14161 cagatggcag tagcgcatat gttcgttttg ctgatttcgc cacacaagac gcaccaaccg
    14221 aagtttgggg cacacatttt accgcgcgaa ttgcgccaga cgcgatcaac aaatggctaa
    14281 gtggattttt ctcccgcgaa gtgcaattac gctgggtggg gccacaaatg acccggcgcg
    14341 tgaaacgcca caacactgta cctctgtcat ttgctgatgg ctatccttac cttcttgcta
    14401 acgaagcctc gttacgtgat ctccaacaac gttgtccggc cagtgtaaaa atggagcaat
    14461 tccgcaccaa tctggtggtt tccggcgcgt cagcttggga agaagatagc tggaaagtga
    14521 ttcgcattgg tgatgtggtg tttgatgtgg ttaaaccttg tagccgctgt attttcacca
    14581 ccgtcagccc agaaaaaggg caaaaacatc cggcaggcga accattaaaa acattgcaat
    14641 ctttccgcac tgcccaggat aacggcgatg tcgattttgg tcagaattta attgcccgta
    14701 atagcggcgt gattcgcgtt ggcgatgagg tggaaattct ggcaacggct ccggcaaaaa
    14761 tttacggcgc agctgccgct gatgataccg ccaacatcac gcaacaaccg gacgcaaatg
    14821 tagatattga ctggcaggga caggcatttc gtggaaataa ccaacaagtg ttgctggagc
    14881 aattagaaaa tcagggaatt cgtatccctt attcttgccg cgcgggcatt tgtggaagtt
    14941 gccgtgttca gcttttagaa ggcgaagtca cgccgctgaa aaaatcagca atgggcgatg
    15001 atggcaccat tctttgctgt agctgtgtac cgaagactgc ccttaagctg acgcgttaga
    15061 ctgcctgttc gaggctgaag ctgtcaacat tcacctgcgg tttcagcctg tcgttcatga
    15121 ttttaatggc gtcgcccaac agcatcgcgc gacccgcaat aacaacgcag ggttgtgcca
    15181 gcaggcatag cgcggcattt tcgccagatt caacgaccag caaattgacc tgctcgtgag
    15241 tatcgctcaa ccaaacgcag gccgcatcgc ccggcatggg cacccacatt tcaccatggg
    15301 agacaaaatg ccagcttttc ggcatttgcg gtttgagata acgaattgct accagtgcat
    15361 tcagcaccag ttcggctttt tggtctttag ataaattaaa atcgcggcac ttttcttcaa
    15421 aagagaaata gagcgcggca tcatcaacac aaaagcctgc gggagaaaag gcatctggag
    15481 tcaacatttt gcgcgcaaaa cgtgagcgaa atagcatacc attggctaaa tcgagcatca
    15541 ttcgatcgtg ctcttcatcg taataccaac gccagttatc gtctggttta attcgcatct
    15601 gcctctcccc taccctataa aaccgcagca acacaatcca ttgtcgcttc gcttaataac
    15661 ctcaacaata aaagtacaaa atgtctaaat aagcaacaac gatgaatata aaacaaccag
    15721 ggctgaaaat aaagccctga ttatcttata aagaggaatt cgaagaaata attagatatg
    15781 ggtaacgatt tctttaatca gcggcggacc tttaaaaata aaaccagaat aaatttgcac
    15841 cagtgaggca cccgcagcaa tcttttcacg cgcagcgata accgagtcga tgccgccaac
    15901 accgatgatc ggtaagcgac cgtttaattc tagtgacaag cggcgaataa tttctgtgct
    15961 ttttaactga agcggacgac cacttaagcc accggtttga tcgcaatttt tcattccctg
    16021 aaccagagaa cgatcgagag tggtattggt tgcaataacg ccatcaatat tatggcgaac
    16081 taaactatcg gcaacctgga tcaattcgtc ttcagaaaga tccggcgcga tcttcactgc
    16141 gatcggcaca tatttatggt gcatcgcttg caaatcattt tgcttatttt taatcgcggt
    16201 taagagatca tccagcgctt caccatattg cagcgtgcgt aatcctgggg tattcggcga
    16261 tgaaatattg atggcgatat atcccgcata ggcatagatt ttttccatac aaatcagata
    16321 gtcatctttg ccctgctcca ctggcgtatc tttatttttg ccgatattaa tacccaagac
    16381 gccgtcataa tgggcctttt ttacgttctc tacgaggtta tcaacgccaa gattattaaa
    16441 gcccatacgg ttgatcaaac cttcggcatc taccagacga aagagacgcg gcttgtcatt
    16501 accaggctgt ggacgtggcg tgacggtacc gatctcgatc gatccaaatc ccatcgcgcc
    16561 taacgcatca atgcactccc cgtctttatc aagaccggct gccagaccaa gcggattttt
    16621 aaacgtcagg cccatgcagt taacaggttt cgcaggcact ttctgccgca ccagtgcttc
    16681 aaacggtgtt ccggtaatgc ggcgtaattg ctgaaaagta aactcatgag cgcgctctgg
    16741 atcgagctgg aaaagggctt tacgaacgaa ggggtagtac atgaactctc ctggattccc
    16801 ggtgtgcaaa ccgggggcgt attatgtgcg agttcggacc aaaagggaat tgacctgcgg
    16861 caaaaaaaaa gcaaacgttt tcagaacctg tttcagggca gattttgctc cttctttgca
    16921 ctcaacccgc cgtaatcatt tatcgtccgc caggcgacat gacacggtgc tattacctga
    16981 caccagtttg tctgacgctg gcttttaggc ggcacgactc ccgcattact taacgcttta
    17041 ccatttacgc tcacctgaat caggttaatg tagtaaggcg ttgggttagt gagttgtaca
    17101 ccctgcgaat tccgtgtcca gcgtaattgt tgataagctt ccagcgggtc gcccggcaaa
    17161 ccttcgggtc gccagaacag tttaaatacc gagcgcatcg ccacttttac gctctgattt
    17221 tcaactttgc cggatggcac gctggcaatg cttaactcaa atagcgtttc gcgatccaca
    17281 ggcaagatgt cgctttccgt tctcagcaag cgcaacgtgc ctgtagtacc gggcttcagg
    17341 agaataagtg gcggagcggc cagtaatagt gctggcactg tcgacgcttc cccccccccg
    17401 gcccaacgcg ttgggcgatt gattttgctg ttaataagcc aagattcctg actggtatta
    17461 gtcagtaaaa tagatatcga ttctctgtct gccggaaaga taaatcgcgt tccgccgacc
    17521 accactcctg cctgcaattc acccgccacc agcagcgata aaacggcgaa aatcaagagc
    17581 gctaatctgt tccacgtatt agtcataatg aagtacaaat gtcgcgctgg cattcacttt
    17641 acccgactgc acatcgctat tgacaggtcg caattgcgca taaaaagtta atggcacctt
    17701 accgctatgt aggctgtagt ctttactcgc ctggttaagc ggaatcaata tcttcgcatc
    17761 atccagaatc gcaattgcca gcccactggc tgcatccgta ccactttcaa tggacagcat
    17821 ttcgctctgc actccgtcgg gggttcctga aaaggtgaca tcaaccgtgc tgacatctgt
    17881 tccgcaatcc tgcaaattaa tgatgaaaga tttctccggg ccataattcc catgaaaaaa
    17941 acgtgtcagc gagacatcgc ctaactgcac attgatttgc ttgctatcct gtgccagcac
    18001 gcatgtatta cggcgaaacg atccgctaat gttgaccgtg ccatcatggg caaatagctc
    18061 acagcccccc gttaatccca gtaagaacag aatccagatt atccgtttca acatcactcc
    18121 ttcactggta aaccagatca aaatagagaa ccgccgtcgc attaccgccc gttgctcccg
    18181 cctttgtgga cttataacga agttgatatt tgagtgtgtt atccccggct tttaagggcg
    18241 taagaggctg gacctgattg agcgggattt cttgctgact ttgcgcatca agaatttgca
    18301 cggcaatccc cgttgccata ccaccgcttc cgcctgtgtc agttagtttg agcagtgtcg
    18361 gcgcaagggt gtcagcctcg ccgctaaaaa ggacgtccgc gccagtaata ccagcggcac
    18421 aaccggtgat atcaatattc aaatcagcag cagtgcttac ggtattcgcg gcctgaaaat
    18481 cactgatatt aaaataacca atatgaatat tttgtaatgc acttttggtg gcgacatcac
    18541 agccctgatc gaccacattc ccataaatat gcatactgac ttgttcaggc ccagccatcc
    18601 cgacatggat gttaagaagg ctaaagaaca aaataacgca ctgatatatc gtttttttct
    18661 tcatacgttc acgcgttaat caaaatttat tctcaccgtt gccgtggctt caaatcgccc
    18721 ggtttctggt ggtacgcctg tcgtgctgac tggatagaca ttcattcgcg gatactgtct
    18781 gcatcatcag gaatagagag cggtagtgtc ccaccagcag gggaaataat gttgttctgg
    18841 ctatcggtca ccaccacgcc cacgccagga cgtgacgtct taatcgcctg gggatagctg
    18901 gggtctgagg ttgccgtcag actcagattg acgttaacta accctgcgtt tcccgtgtta
    18961 cacctgaccg gaattgcaag ttctgccgtt ctggccccaa gaggcttatg ccctacaaca
    19021 cgaaagttag ccgcaaatac gtcgcccaga tcaacctcga ttgtttctcc ggcattgacg
    19081 gaacatgagc caagtgaatt gatcgtacca gacaggtaat acactaaaac cggtgtacca
    19141 gtagtcgtgc aggaatcacc tgcgggtatg ttgtagcatt caaataatcg cgccagcgcc
    19201 ataggaggaa tgacgaccga accaacgaac ggctgcaata ttttcaggga aaagctaccg
    19261 ctattaccgc tagccccctg gccacctaaa cgtagaccgc cgggatcgca aatatgccat
    19321 cgtgtatctg cccctgtgcc cacgttttcg aaaggaaccg cagtaggaga actgccttta
    19381 acctgcaata aaactttggc ggcaagatag tcattaattt tgtaccagtt gtcgcctaaa
    19441 tagactaacg gtgaatccgc ggcgaatgcc catcggccac ttctgacatt gtctttatcg
    19501 caatcacagg aaacataata atcattcgat tcgttccact gataaaattt gtctttaact
    19561 actccagccg tattttcttc ttcatccaga gttttaacgc caaaattgac gccaatatac
    19621 ggattcccct gagtggtatg acactgcccg atataagcag cagtttgagc gcaccatagc
    19681 attaagacgg cggcaaaaaa tagtcgtagt aataacacgc atttttctcc aaagattatc
    19741 tgcatatggc atccattcgc gtaatcgcag agttaatttg ttgttcggta agctggtaac
    19801 tgatatgaca aatttgatcg ggagcttcgc cccagcgtac agttatcgct ccctgagcgg
    19861 gcaaaccgga catatagagc gagccatcat catcaataat gccgctatta gtctgtatgc
    19921 cgtccagcgt cgctatcgcg ccaaaacgca gcggtatacc ctgctttgat gttttcatta
    19981 atacccttgc tcccgcatgg gcattgaact ccgccagaac caacgcccct ttggtgggaa
    20041 ccacgttgac tactgcattt tcaagatcca cgttacgttt taacgatgcc gcatcaagtg
    20101 cgattcgatt ttcccgataa gaagtggcat aggggacaac ggtatatccg cgccagtcag
    20161 ttttcagccc ggtgctgttc gccacacgag tattctctgc gcctggtgct gcaatcagca
    20221 cgttagtttc ccccagcgat tgccccaggg taatgccctg tgaatgggca actaaactgc
    20281 ctgaaagagc gtagttcagt tgttgttgac tgccgttatc gctgtagttg tagcccactc
    20341 gggcatctgc aaacgcccct ttgtagtcca tgctggcgct accattagcc gttttcccct
    20401 cgctgttata tccctgttgc acgctgtagc tcaggttacc gtcatcgagt agtgtctccg
    20461 ttaaacccag gttttgtgta atgtgccctt cgttatcgat actggtgtta gaggtcatcc
    20521 aggcgtaatt ttttttcctg gtatagctat cattacttct ggggagcaac agattgagcg
    20581 gcagggaaaa atttagtgca aacacctgat cagcatcagg ttgaccacgg gacttactgt
    20641 agttccagga aacccccagc gagagatctt taatttgcgt gttgtagcca aactgtaata
    20701 gccgatcctg ttgatcggta tgccagtaag tttgctggct accacttaaa taaaacaaac
    20761 cgtactcgcc taattgctgg gagatattga cctgcagttt gccacgtttg gtgtaaaaca
    20821 aattgtaata acgcgaccac atcggcgtat cttcatcatc accgtcatta aattcgtagc
    20881 catccatatg tttatacatg gtgtcggaaa gggtgtagaa acccgacgtc gaatagcgat
    20941 agcccatcaa ttgaaaatta gtgcccaatt ggttgagcgt tttggcatac agaaaacgat
    21001 atgactgacc tttatgttcg ctttggtctg ccagcgtact cttcgcctgg gtcgcatcga
    21061 acgaaattgc tccgaaatcg ccaaggttaa atcccagacc aaacatggcg gcacggtaat
    21121 attcagcata ttgtccacca ccataccatg tcgttcccca cggtccgccc cactgcaacg
    21181 tggtctgggc aaatttgctc tcctgctgtt cattactatt ggttctgtat ttcgccagtg
    21241 tcaccgcgta tttgattcgc ccctgacgct ggagtaatgg cacgctggaa aacggtacgc
    21301 tgtagctatt gacgctgccg tccgcttctt tgatttcaac taacaaatca ccgctcgacg
    21361 acgtggaata gagatcacta atttcaaaag caccaggcga tacataagtt tggtaaatgg
    21421 tgtaaccatt ttgcttaatc gttatctggg cattgctttt ggcgatacca cgcaccacag
    21481 gcgcaaaccc tttcaggctg tctggcagca tgttgtcatc tgacgccagc tgcacaccac
    21541 gaaagctgac actatcgaaa aaatcgcctg cagtataatc atcacctacg gtcagttcgc
    21601 cttttagtgg aataaccgcc cgctgcaaat agctgctgag attcttccat tctgcggttt
    21661 ggccactact gcgactccat gttgaattgt tgcgcaatct ccatgggcct aaattaacgc
    21721 cactgtttaa attcagaaaa tagctgtcgc cagaatcact gtctgcgctg ctatgaatac
    21781 tgttagcccc gctaaatagc gcgttgatcc cttcatccca acgttcaggg gggacataac
    21841 cacgggcatt acttttgatg gcgatttgcg gtactgaaat ttccagacgg agtttattca
    21901 cattaaacgt tacgctggca tccggaataa tctccgccaa tggcacacac acttgtttgt
    21961 ccagttgttt aaaagccgga aacgcatcag tattaacacc cattgcatcc aggctttcag
    22021 tcgtcaaaca ggcggcaatc cccttctctg gcgttatctc aacaaaattg acgttgcgcg
    22081 tatctacaat tgtctgattc agaacaatgg taatgactcc aacttactga tagtgtttta
    22141 tgttcagata atgcccgatg
//
LOCUS       DAEEUE010000063        21701 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.063, whole genome shotgun sequence.
ACCESSION   DAEEUE010000063 DAEEUE010000000
VERSION     DAEEUE010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21701
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.063"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            58..1059
                     /locus_tag="KLK84_002584"
     CDS             58..1059
                     /locus_tag="KLK84_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBE2009859.1"
                     /translation="MHKSNCFDTARLVAAMMVLISHHYALSGQLEPYIFGFESAGGIA
                     VIIFFSISGYLISKSAIRSDSFIDFMAKRARRIFPALVPCSVLTYFLFGWMLNNFSTE
                     YFSHDIIRKTISSILMSQAPDADITSHLIHPGINGSLWTLPLEFMCYIITGVAVALLK
                     SGKTFIVILLIFVSLSLVESVSENKDVIFSIPVWLYPLRGMAFFFGATMAMYEKSWNV
                     SNVKISVISLLAMYAYASYGKGIDYTMACYILVSFSTIAICTSFGDPLVKGRFDYSYG
                     VYIYAFPVQQVIINTLHIGFYPSMLLSAIVVLFLAHLSWNLIEKRFLTRSSPKLSLN"
     gene            complement(1350..1784)
                     /locus_tag="KLK84_002585"
     CDS             complement(1350..1784)
                     /locus_tag="KLK84_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBE2009860.1"
                     /translation="MRITLQWDINEMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINSQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(1756..2547)
                     /locus_tag="KLK84_002586"
     CDS             complement(1756..2547)
                     /locus_tag="KLK84_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE2009861.1"
                     /translation="MAINNFKPFALDPNANVTSQADWEALPALLSGFTAGKASSAQVN
                     KAIRQASFIAAALAQYTANKSRLDVLDDGDLNGFISKMGTAFGKDFQALDATLSALAG
                     LATGANKLPYFTGNDTAAQTDLTSVGRDIIGKNTIADILTYLGLGEAAKRNVGTGANQ
                     IPDMSLFASSNTATAAAQKFPSGLILQCGQLNGSPNVSSTYGMRFPMTFSRVLAVTVT
                     LNVTGASGQPTVSATNVHNTGFDITVSPGSGYGSSADAYYIAMGY"
     gene            complement(2547..3227)
                     /locus_tag="KLK84_002587"
     CDS             complement(2547..3227)
                     /locus_tag="KLK84_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HBE2009862.1"
                     /translation="MSKYTELITNYHATKPLFFDHIDLSTRPLIDVSSTMSGLITAFD
                     IDTAVGVQLDILGLWIGRSRIVSQPISGVYFSWDTDGLGYDQGIWKGPYDPDSGYTTL
                     SDETYRIILKAKIAINNWDGRNDSLPPILDAATEGSGLRMQIVDNQDMTISVWVFPET
                     DISDVSLELIAAIKQGYLTVKAAGVWAGDVETPSVEAPSEGSKFFGFDMDNEYIGGFD
                     VGAWGTIL"
     gene            complement(3224..4423)
                     /locus_tag="KLK84_002588"
     CDS             complement(3224..4423)
                     /locus_tag="KLK84_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001197067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBE2009863.1"
                     /translation="MALNLDTLGLSATVTAQGISAPDYQTILDTLTSYFRQIYGSDAY
                     LEPDSKDGQMVALVALAVHDANNTAIGIYNSFSPTTAQAAALSSNVKINGITRKVATN
                     STADLLLTGTAGTTITNGSARDKNGIIWNFPASVAIGVDGTVLVTATCANSGSVAALA
                     GTITTINTPTRGWVSVTNPVAATVGSPAETDAELRIRQGQSVALPSITPFEGVDGAIA
                     NVAGVTRHKLYENDTGATDSNGLPPHSISAIVDGGDVTEIAQTIRGSKGQGTATYGKT
                     SVTVPDTYGNPHVISFSRSTDVPIFVAITLKVFTGYTSQIGEQIKQAVADYINGLTIG
                     DDVLLSRIYSPANLGVVSGGNARYYDITDLLIGKSSGSVSASNIDIAYDASASCSTAN
                     ISITVTS"
     gene            complement(4424..4777)
                     /locus_tag="KLK84_002589"
     CDS             complement(4424..4777)
                     /locus_tag="KLK84_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009864.1"
                     /translation="MRYRREDTEGDYTFGQGDDTFLIDSPECVAQAVKTRFELWRGQW
                     FLDLTEGTPYVQSVLGKQRSDVYILAIRERIQDTPGVLSILSFDTNYDGTSRRVTFTS
                     SIDTIYGQTTVTSEA"
     gene            complement(4777..5517)
                     /locus_tag="KLK84_002590"
     CDS             complement(4777..5517)
                     /locus_tag="KLK84_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HBE2009865.1"
                     /translation="MPIPTQSQIGGEQQTAQAIADSVSTQMRVAMPGIIQSFDPETVT
                     CTVEVALRGIVGDGSTELKPLVDVPVIFPRGGGCTLTFPVKEGDECLLIFADRCIDFW
                     WQSGGVQETVDPRQHDLSDAFAIVGPQSQARKISGISTSAAQLRTDDGAAFVEVAAGH
                     NITIKTPGQLTATAEGGTTITSPTITLNGNVTINGNLSQGMGESGGTATMLGPVMVTN
                     DVTASGISVATHKHGGVQTGGGTTGGPQ"
     gene            5740..6030
                     /locus_tag="KLK84_002591"
     CDS             5740..6030
                     /locus_tag="KLK84_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HBE2009866.1"
                     /translation="MGFRFRKRIRIAPGLAINISKSGVSTSIGGKGSTINIGKKGVKM
                     TNGLPGTGLSHTTNLYSPGKSTEKKQLTHKQKIIRNILFVIIVFIIIKAQYF"
     gene            complement(6054..6398)
                     /locus_tag="KLK84_002592"
     CDS             complement(6054..6398)
                     /locus_tag="KLK84_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009867.1"
                     /translation="MRHLLCTVTVFAVLISAPAFADQQCGDFKIHWADDGLARINGAK
                     PEMQKITFLKNKGDYNNIKMDWRMATDQPGRWVGLEYINRNGKAILNAQWLQASMDSP
                     RQYATYDCRKVK"
     gene            complement(6411..7475)
                     /locus_tag="KLK84_002593"
     CDS             complement(6411..7475)
                     /locus_tag="KLK84_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009868.1"
                     /translation="MSLNWMRHFELQLLDQNGQGVSLSDFKVTFQIEWADTRWPRVAN
                     VKIYNLSTDTTNKILGQEFAKIRIIAGYDGIAPDVDASQVGVSREISPDQIGQVNGQN
                     YGLIFDGDIRFTVTGKDNITDSWVLIQAIGDHEAFLYATTITTLAAGYTVADLHRATM
                     QDFNAFGVTQGITGDFPDTVFPRGRAIYSSSRNVMDNIAAQCKATWQLVDGQVQMVPE
                     DKYIHEAIVLNANTGLIGMPQQTMGGGVNVRCLINPNIRINGLIQLDQASVYRATLGN
                     SEIAQSPGRITETEENGNRVLTGTTSQAASIATDGVYIVKAIDYTGDTRGQAWYMDLM
                     CFARGARDLYSPKAMQGTTN"
     gene            complement(7478..7783)
                     /locus_tag="KLK84_002594"
     CDS             complement(7478..7783)
                     /locus_tag="KLK84_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009869.1"
                     /translation="MQFNEIPLSPDNQQFRVLLGNTTYTLRIIWRDAAGWIMDVMDSG
                     GAALLSGVPLLTGVNLLRQYPQLGIDGALVVATDKGAPDEPTKTNLGTYSHLIFVQE"
     gene            complement(7785..8387)
                     /locus_tag="KLK84_002595"
     CDS             complement(7785..8387)
                     /locus_tag="KLK84_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009870.1"
                     /translation="MDILSAIFRQQSRRIGILIPSVVVSEKHSDALEITEHPVEKPTT
                     NSASGFIADHAYKRPSEVTMECGFAGGGSLLDFIDTSSIGLSAGLSPKETYQQLLDLQ
                     SSRVPFDVVTGKRVYSNMLVRAIEVTTDKTSENVLNCTLTLREVIMSQTQSVSVADKS
                     DMQDGVSTSAVQNSGMKSTTPPNESLLSQLGGSVTSAFGG"
     gene            complement(8387..10402)
                     /locus_tag="KLK84_002596"
     CDS             complement(8387..10402)
                     /locus_tag="KLK84_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HBE2009871.1"
                     /translation="MSGNADTIKDFLVSLGFDIDQAGANKFEAVLKGVTANVLKVGAV
                     VKGAALSIVGFTTQIANGLDKIYWASQRTGASVQGIKALGYAASQTGASAESAMSSLE
                     GLAGFMRSNPGAEGFLNRLGVQTRDASGKMRDTAAIFTGVGQKLNNMPYYRAKQYAQM
                     LGIDENTLMAMRRGMGQLSSEYALTAKRIGFNAESAAKQSNIFMTSMRNLTMTLGQAK
                     DKIGSNLAGGLAGSIDNFRRQILDNWPKIEAVITKIIKGILWAGDAITRVLWRTGQAV
                     EGVIAWFKKLNPATQQLIALFSGLLVAWRLLNTAFMSSPLGMITTLIIALGLLLDDYQ
                     TWKEGGKSLIDWGKWKTEIDQAVKMIGDLKKTVTDLTKALAKLLGIDPKSWSLKWDFS
                     NFISQMGEFGKMLNMIADLLNAIKDGRWADAASIGKQMLNQGSENPSAMPMVTDSANG
                     TADWIKEHWGFDPRSVGRTVRGWFGDDEPEQLGQSVKRPQPTKAGSELLGWMQPMFTN
                     LEQLYRLPEGLLRSVAITESGGNQFAVSGAGAKGLFQFMDGTARDMGLRGNDVFDPEK
                     SAQAAAKYLSQLLRQNGGDLSKALASYNWGIGNVKRYGMGLMPQETRNYIPKVMSNMP
                     TSAPVIQQETNINIHGVFDPREAARLTVDRQKGVNSQLTQQLPAGPR"
     gene            complement(10580..11005)
                     /locus_tag="KLK84_002597"
     CDS             complement(10580..11005)
                     /locus_tag="KLK84_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009872.1"
                     /translation="MEFEIKGVKYRTAKLSVFEQLKVSRKLLPVLAGMVSDFRSVQEK
                     ISSKDTEGAMATILPKIANAVSDLSDGDVDAILFPCLSVVSREHMKGWVPVCQHGEMA
                     FDDIDLLTMLQLVARVVADSLGNFLQGLPTSETPTPLAE"
     gene            complement(11009..11449)
                     /locus_tag="KLK84_002598"
     CDS             complement(11009..11449)
                     /locus_tag="KLK84_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3277 family protein"
                     /protein_id="HBE2009873.1"
                     /translation="MATYSFLDVTASLTGPTGVIDLGQGSANSEEGITQTMGGNKNTM
                     TIGADGEVMHSLHADKSGTITVTLLKTSPVNKKLSLAYNAQSQSSATWGNNVIVIRNT
                     ASGDISTARSCAFQKQPDFNNAKEGGTVAWVFDCGKIDQLLGEF"
     gene            complement(11460..12605)
                     /locus_tag="KLK84_002599"
     CDS             complement(11460..12605)
                     /locus_tag="KLK84_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 domain-containing protein"
                     /protein_id="HBE2009874.1"
                     /translation="MAQGLPVSNVVNVDVIMSPRAASGRNFGALLILGPSTIIPVSER
                     IRRYSAAEDIGKDFGVESPEYKAAQVFFSQSPKPQEVFVGRWVKTKGDSEQATPETLE
                     QAVNAMLDYTSWYGLGIADDEDIPDADWLKVAAAIESSSVSRILAITTSDEKCLQTAS
                     SDDLASKLKTAGYSRSFIQYSSGNKYAALSAFGRAFTVNFNGSNTAITLKFKQEPGVG
                     YETLTVSQASALDAKNCNVFVYYANDTAILQQGVMANGDFFDERHGLDWLQNYVQTNL
                     YNLLYTSTTKIPQTDAGVTRLLSNVEQSMDQSVTNGLVAAGVWNGGPIGQLNSGDTLT
                     KGYYVYAQPLSEQAQADREARKAPLIQVACKLAGAVHYADVQINVVR"
     gene            complement(12609..13166)
                     /locus_tag="KLK84_002600"
     CDS             complement(12609..13166)
                     /locus_tag="KLK84_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009875.1"
                     /translation="MGELRLSNNTSTERGWLIPTSGDPDYDEALDRLLSQWMRNVSGL
                     SAGMVRPRWQKEQPPLLPVETNWCAFGVIGWSGDDSPAFTRQTDDGSQLWRHETIECM
                     ASFYGPAGMVYASRFRDGISVPQNNAALNALGLSLGDYTGLFPELINQQWVRRYDMTV
                     RLRRKVVREYGIKSLVEAPVIFFGD"
     gene            complement(13141..13530)
                     /locus_tag="KLK84_002601"
     CDS             complement(13141..13530)
                     /locus_tag="KLK84_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor"
                     /protein_id="HBE2009876.1"
                     /translation="MPTLDVTDVLFDPDFCDFNLWVTRRVQTVDEDGIGSDSEVKKQF
                     AGVVTVDRSLENRRMQAGQVISGAILIVTTERLTQGQTGRDADIVTYQGRDYRVTFVD
                     PYTAYGAGFVQAHCELMPFDGGTPVEQ"
     gene            complement(13517..14071)
                     /locus_tag="KLK84_002602"
     CDS             complement(13517..14071)
                     /locus_tag="KLK84_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009877.1"
                     /translation="MSFKSGVTTRVDNAQAILDALRSLTKKDVLVGIPEEDSEREDVP
                     FGNAGIGYVNEYGSPAQNIPPRPHLIPGVKSVEEQTVPQLKAAAQAALDGNAAGAERA
                     LNRAGTLAANGVRRYMTITGFTPLADSTVEARARRGRKGATLELARRVAGESPGTDLA
                     KPLIDTGQYRRAITHVVRDKDADS"
     gene            complement(14068..14475)
                     /locus_tag="KLK84_002603"
     CDS             complement(14068..14475)
                     /locus_tag="KLK84_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HBE2009878.1"
                     /translation="MPKNQSLPAVSDFRRDFPQFADPAKYPEAQIQFRLNLADELLSE
                     NVTGKDLFPYFAGLFVAHYMTLWAADSRAMLVGGPGGSTNGVQSSKSVDKVSVSYDTS
                     ATLNPDAGFWNNTRYGAEFYQLITMFGAGGRQL"
     gene            14474..14708
                     /locus_tag="KLK84_002604"
                     /pseudo
     CDS             14474..14708
                     /locus_tag="KLK84_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14705..15646)
                     /locus_tag="KLK84_002605"
     CDS             complement(14705..15646)
                     /locus_tag="KLK84_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HBE2009879.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLAGYTWTRDIQLRE
                     DVSIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVVELAAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWAISTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTYLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRMVAYTNDKKYVRYPLVPL
                     QSVPIQYRGLYQIATYYGKLGAVEPVYKETISYVDGI"
     gene            complement(15658..16161)
                     /locus_tag="KLK84_002606"
     CDS             complement(15658..16161)
                     /locus_tag="KLK84_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009880.1"
                     /translation="MTAYLYRMPVGIAGAISRPQDLTVEPVVLKSDNAFAAYGLAGKY
                     DADGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVNL
                     GSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDANGN
                     AEISWKI"
     gene            complement(16166..17386)
                     /locus_tag="KLK84_002607"
     CDS             complement(16166..17386)
                     /locus_tag="KLK84_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HBE2009881.1"
                     /translation="MKYFFNTRLGETRYQLADGSLLCKDVPIGRTGKQLYGAADLPNL
                     KPDKLGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGDHSDLMLADLIVKDENAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKSWWTRMRTAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKVPEMGGGPTGDSDDDLKTLLKALLAKLEGNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTVFKREVLAS
                     ADKTLVRQVVGDADIRKLPKQSVDMAFNAVSEIAKGRNTRSTTGDAQRPNMGMTSIAS
                     LNKQNAEFWSNRKG"
     gene            complement(17401..18138)
                     /locus_tag="KLK84_002608"
     CDS             complement(17401..18138)
                     /locus_tag="KLK84_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBE2009882.1"
                     /translation="MATNRQSRQAEIRYRTSLRQIARAVGDIVNGRYDGSNDSVTEIM
                     DALERYSEIITPWATKVAENFTADIARQNEKQWRQHSRNISAELRNMVDRAPVGQVMK
                     SIVAEQIKYIKSLPLEAADRVYDIQNKAIEAVVAGGRAEPFAKEIAASGDVSRSRANL
                     IARTELGRATGALDQARALSIGSNGYIWRTAEDGDVRHSHREMEGKFVEWGKPPTLDG
                     MTGHAGELPNCRCYKEIVFPNPHSYLA"
     gene            complement(18026..19492)
                     /locus_tag="KLK84_002609"
     CDS             complement(18026..19492)
                     /locus_tag="KLK84_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HBE2009883.1"
                     /translation="MARNKQATRRTAQATADGYENFVARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRLETIGEGKFKGILPLDRWMINPVLTRR
                     IKEMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAFEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRVSSLQERRLRLPMRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVAVNTTNAISTALGDGLMTRKAAMTDLRENSDVTGIGASITDEDIENAEDEAPPGIG
                     ELGDKPPEPPGGDPISNEPTADSAGGRGYRKWALRWFK"
     gene            complement(19492..21114)
                     /locus_tag="KLK84_002610"
     CDS             complement(19492..21114)
                     /locus_tag="KLK84_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerL protein"
                     /protein_id="HBE2009884.1"
                     /translation="MPLPFPFDFKHPDYQMVFEWRMERLQRIRQNPEILPALKQFYRT
                     NPAQFIIDWGMTTDPRNIDYGLPVTIPFLLFPKQEEWIHWIMERWGNRENGITEKSRE
                     MGLSWTAIGLACSLCLFNKEMVIGFGSRKEEYVDSTGDPKALFWKARKFVETLPVEFR
                     GSWDEKKHAPYMRVEFPETGAVIKGEAGDNIGRGDRTTLYLVDESAFLQRPLLIDAAL
                     SQTTRCRIDLSSVNGMANPFAQKRHGGKIPVFTFHWRDDPRKDEEWYRRECEKIDNPV
                     VVAQELDLNYSASAEGVLIPSEWVQAAVDAHIKLGIQPTGKRLGAMDVADEGRDKNSF
                     STRHGFLLENVREWSGVGSDIYQSVEKVFGFCEQDNLEEFRFDEDGLGAGVRGDARAI
                     NELRNAARRPSILATPFRGSGAVFDPDDEAVRGDNGQAARLNKDFFANAKAQSWWRLR
                     KLFQNTWRAVVEGMDYNPDEIISISSSMALKDKLIIELSQPTYSINGVGKIVIDKQPD
                     GTRSPNLADSVMISYAPMNSALNIWELLGRQA"
     gene            complement(21117..21689)
                     /locus_tag="KLK84_002611"
     CDS             complement(21117..21689)
                     /locus_tag="KLK84_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBE2009885.1"
                     /translation="MFRRCDLKQLTPKQELFCREYLKDLNATQAAIRAGYSEKTARAT
                     GSENLSKPDIADRIAELKAERNEEVGIDAAYVLRRLTEIDQMDVLDILLANGELKQIK
                     DWPKVWRTTLSGMDVVEMASADSAALLKKIKWPDKVKNLELLGRHVSVQAFKDNVKNE
                     VTGADGGPVRTEITNLTPEQAAEAYRKMMG"
BASE COUNT         4927 a         5929 c         5385 g         5460 t
ORIGIN      
        1 ggactatgac ataaagatct gatattttcc tcacaaaaac ttagggattt tattttaatg
       61 cataagagca actgctttga tacggcaagg cttgtggcag ctatgatggt tctaatcagt
      121 caccactacg cgctctctgg acaactcgag ccgtatatat tcggctttga aagtgctggc
      181 ggcattgctg ttatcatatt cttttccata tcaggatacc tgatttcaaa atcagcaatc
      241 agaagtgatt cctttattga tttcatggct aaaagagcaa gaagaatatt ccctgctctt
      301 gtcccttgct ctgtactgac gtatttttta tttgggtgga tgcttaacaa cttcagtaca
      361 gaatatttta gtcatgacat tatccggaag accataagct ctattttaat gagtcaggct
      421 ccagatgccg acattacatc acatttaatt catcccggta ttaacggcag cttgtggaca
      481 ctaccactgg agtttatgtg ttacataata actggagtgg ctgttgctct actaaagagc
      541 ggaaaaacgt tcatagttat actattgatt tttgtttcac tttcattagt tgaaagtgta
      601 tctgagaaca aggatgtaat attctccatt ccagtatggc tgtatccttt gcgggggatg
      661 gctttctttt ttggtgccac tatggcgatg tatgagaaat cttggaatgt aagtaatgtg
      721 aaaatttccg tgatttcttt gcttgctatg tacgcatacg cgagctatgg caaaggcatt
      781 gactatacaa tggcttgcta tattctggtt tctttctcaa caatagctat atgcacatcc
      841 tttggagatc cactggttaa gggtaggttt gattactctt atggtgtata catttatgca
      901 ttccccgttc agcaggttat tataaataca ttgcatattg gcttttaccc ttcgatgttg
      961 ttaagtgcca ttgtcgttct tttcttggct catctttctt ggaatctaat tgaaaaaaga
     1021 ttccttacac gaagctcgcc aaaattatcc ctgaattgaa taataaaggg gccatcgagc
     1081 ccctgaataa atcaaagcca gatataacac tgcctggcag tggacaccgt tctgatgcct
     1141 cgtcctgcaa ggacgagcga aggtggagtt cttttccacg cagctattta gtacagtact
     1201 gagtcatatt gtttatgtat gagttaactt ccgtaatatc aattaaataa aataagttga
     1261 ggccattgta gaagtgagca ttatatccag gttatctgga cctgttgaaa aatgcgtatt
     1321 attccaacaa ttaccctaga ttaacaagtc tatttatttg gatgcatagg ccactcgggt
     1381 tttactacat taacacgcat aagcagtacg cgatattttt tccatgccag taacagtttc
     1441 gtttcatcat ctgttgccat acctaaatca accgcatcct gaagcggcgc tattacattc
     1501 gttgcctctg ctatcagtgc agatttcttt tcatcattta ttaaggctaa ttgttctggt
     1561 gttggagaag gacggtcaac aatgacaggg tgaccgttct caccacaggt aatcattttt
     1621 gttacactct gtgagttaat cagtgttttc cactctgatt ccgagatttc cactgcatca
     1681 tcaggaatat tggtgccgtg aaattcagtc gaataaaatc cgtttgtcga tgcagaataa
     1741 aaatatgaca tttcgttaat atcccattgc aatgtaatac gcatcagcag atgaaccata
     1801 tcctgaaccg ggcgacactg taatatcaaa tccagtgtta tggacatttg tcgccgatac
     1861 agtcggctgc cctgacgcgc cagtaacgtt caatgtaact gtgacagcaa ggactcttga
     1921 gaatgtcatc gggaacctca tcccgtatgt tgaagataca ttcggggagc cattcaactg
     1981 accgcactgt aaaattaatc cagacggaaa tttttgcgca gcagccgttg cggtattact
     2041 tgacgcgaac aggctcatat caggtatctg attcgcccct gtacctacat tccttttagc
     2101 cgcttctccc aaaccaaggt atgtgagaat gtcagcaata gtatttttcc caataatgtc
     2161 acggccaaca gaagttaaat cagtctgcgc tgctgtatca tttccagtga aatatgggag
     2221 tttatttgca cctgttgcga gcccagctaa tgccgacagc gtggcatcaa gcgcctggaa
     2281 atctttcccg aaagcggtcc ccattttgga tataaacccg ttcaggtctc catcatcaag
     2341 cacatccagc ctgcttttgt tggcggtgta ctgcgccaac gctgccgcga taaagctggc
     2401 ttgccgaata gctttgttga cctgtgcgct ggatgcttta cctgccgtaa accctgaaag
     2461 cagagccgga agtgcttccc agtcagcttg tgaggtgacg ttagcgttcg gatcaagcgc
     2521 gaaaggttta aagttgttta ttgccattag agtattgtcc cccatgctcc aacatcgaac
     2581 ccgccgatgt attcgttatc catatcaaac ccaaagaatt tagagccctc ggatggtgct
     2641 tctaccgaag gcgtttcaac atcaccggcc catacgccag ctgctttaac ggtgagatag
     2701 ccctgtttga tagcggcaat cagttcaaga gacacatcag aaatatcagt ctcggggaat
     2761 acccaaaccg atatcgtcat gtcctggttg tcgacaattt gcatcctcag gcctgagcct
     2821 tcagtcgcag cgtcaaggat gggaggcaga gagtcgttcc gaccgtccca gttgttgata
     2881 gcgattttcg ctttcagaat gatgcggtac gtctcatcgc ttagcgtcgt atagccagaa
     2941 tcaggatcat atggcccttt ccagatgccc tggtcatacc caagcccgtc agtgtcccag
     3001 ctgaaataaa ctccgctaat tggctggctg actatgcgac tgcgtccgat ccacagaccg
     3061 aggatgtcga gctgtacacc gacagcagta tcaatatcga aggctgttat aagccctgac
     3121 atagtgctgg acacatcaat cagcgggcgg gtgctcagat ctatatggtc aaaaaagagt
     3181 ggcttggtag cgtggtagtt agtgatcagt tcggtgtatt tgctcatgag gtcaccgtga
     3241 tactgatatt cgcggtgcta caggacgcag aagcatcata ggcaatatca atgtttgatg
     3301 ccgatacgct gccagacgac ttaccgatca gcaggtcggt aatatcgtaa tagcgggcat
     3361 tcccgccgct cacaacgccg aggtttgccg gggaataaat acggctcagc agaacgtcgt
     3421 cgccaattgt taggccattt atataatcgg caacagcctg tttaatctgc tcgccgattt
     3481 gagaggtata gccggtaaaa actttcaggg taatggctac gaaaattggc acatcggtag
     3541 agcgcgaaaa actgatgacg tgtggattac cgtaagtatc cggcaccgtg acagaagttt
     3601 taccgtaagt tgcggttccc tgccctttac tcccccggat tgtttgggct atctcggtaa
     3661 catcccctcc atcgacgatg gcggaaatag agtgtggcgg cagcccgttg ctgtcggttg
     3721 ccccagtgtc gttctcatat agtttgtgac gtgtcacgcc agcaacatta gcgatagcac
     3781 cgtcgacacc ttcaaacggt gtgatcgatg gtagcgcgac gctttgcccc tgccgaatgc
     3841 gcagctctgc gtcggtttcg gctggtgaac cgacagtagc cgcaactgga ttggttaccg
     3901 acacccaacc tcgggtcggg gtgttgatag tggtaatagt cccggccagc gccgcaaccg
     3961 aaccgctatt cgcacatgtg gccgtcacca gcacagtacc atcaacgccg atcgctacac
     4021 ttgcgggaaa attccagata atgccgtttt tatcccgtgc ggagccattc gtgatagtcg
     4081 tgcctgccgt accggttaac agaaggtcag cagtagagtt tgtcgctact tttcgcgtga
     4141 tcccgttaat tttcacattg ctgctaagcg ctgcggcctg cgctgtcgtc ggtgaaaaag
     4201 agttgtagat cccgatagcg gtgttgttag cgtcatgcac ggcaagagcc accagcgcga
     4261 ccatctgccc gtctttgctg tctggttcga ggtaggcatc actaccgtaa atctgcctga
     4321 aatagctggt cagtgtatct aggattgtct ggtaatcagg cgcactaatc ccctgggcgg
     4381 ttaccgttgc cgatagcccc agcgtgtcga ggttcaaagc catttatgcc tcgcttgtta
     4441 cagtcgtctg gccgtagatt gtgtcaatgg aggaagtgaa ggtgacgcga cggctggtgc
     4501 cgtcataatt ggtatcgaag gaaagaatcg acagaacgcc cggtgtatcc tgtatgcgtt
     4561 cgcgtatagc caggatgtag acatctgatc gctgcttccc aagcactgac tgaacatacg
     4621 gcgtgccttc cgtcagatcg agaaaccact gaccgcgcca cagctcgaaa cgggttttta
     4681 cggcctgggc gacacattcc ggactgtcga taaggaaagt atcgtcaccc tggccgaaag
     4741 tgtaatcgcc ttcagtatct tcgcgacggt atcgcattat tgcggccctc cggtagttcc
     4801 cccgccagtc tgtactccgc catgtttatg cgtggcgaca ctgataccag aagctgttac
     4861 gtcattcgtt accatcaccg gcccaagcat cgtcgcagta ccgccgcttt cgcccattcc
     4921 ctgagacaag ttgccattaa tcgttacgtt gccgttcagc gtgatagtcg gggatgtgat
     4981 tgtcgttcca ccttcagccg tagctgtaag ctggcccggt gttttaatgg tgatgttatg
     5041 tcctgcggcg acttctacaa acgccgcacc atcatcggtt cgcagctgcg cggcgctggt
     5101 actgataccg ctgattttcc gtgcttgcga ctgcgggcca acgatggcga acgcatcaga
     5161 taagtcatgc tggcgcgggt cgacggtctc ctgaacgccg ccgctctgcc accaaaaatc
     5221 gatgcaacgg tcagcaaaaa tcaggaggca ctcgtcgcct tcttttaccg gaaaggtcag
     5281 cgtgcaaccg ccgccgcgcg ggaagatgac cggcacatcc accagcggtt ttaattcggt
     5341 ggatccatcg ccaacaatac cgcgtagcgc cacctctact gtgcaggtaa cagtctcagg
     5401 atcgaacgac tgaatgatgc cgggcatcgc tacgcgcatc tgggtagaca ccgaatcggc
     5461 aatggcctgc gcggtctgct gctcaccgcc gatctgtgat tgagttggaa ttggcataaa
     5521 acccccataa aaaaaccagc cgaagctggt ttggtatcaa ttatctctta gtaactaagc
     5581 tacatcagtg tgaagtaagg cttttccgct gcaatggcac acattcacaa ggtgtattag
     5641 gatatgtttg caacaagtaa gcattgaaaa tcaatttcag ccatgaagcc gccatagagt
     5701 ggacctgcac catcgcatta ttgactacag gagtaacaaa tgggatttag atttcgtaaa
     5761 agaatccgga ttgcgcccgg actcgcgatc aacattagta aaagcggagt aagcacttca
     5821 attggtggta aaggttccac cattaacatc gggaaaaaag gcgtaaagat gacaaatggc
     5881 cttcccggca cggggctgtc gcataccact aacctttact ctcccgggaa atcgacagaa
     5941 aaaaaacaac ttactcataa gcaaaaaata attagaaaca tcctgttcgt aattattgtg
     6001 tttataataa ttaaagctca atatttttga cgcatgcccg cctatctggc gggctatttt
     6061 acttttcggc agtcgtatgt tgcatactga cgcggcgaat ccatgctggc ttgcagccac
     6121 tgtgcgttga gaatggcttt gccgtttcga ttgatgtact caagaccaac ccatcttcca
     6181 ggctgatcgg tagccatacg ccaatccatc ttgatattgt tataatcgcc tttgtttttc
     6241 aggaaggtga ttttttgcat ttctggcttt gcgccattga ttctggctaa gccatcatct
     6301 gcccaatgga ttttaaaatc accacattgc tgatccgcaa aagcgggtgc tgatataagc
     6361 accgcgaaca cggtaacagt gcaaagtaaa tgtctcacgg ccccacctca ctaattcgtc
     6421 gtcccttgca ttgctttcgg gctataaaga tcacgagcgc cacgcgcaaa acacatcaaa
     6481 tccatgtacc acgcctgacc tctggtgtcg ccagtatagt cgatagcttt gacgatataa
     6541 acgccatccg tcgcaatgct ggcagcctgt gacgtcgtgc cggtcagcac acggttgccg
     6601 ttctcttctg tttcggtgat acgcccgggc gactgtgcta tttcgctatt gccgagcgtg
     6661 gcgcggtaca ccgaagcctg atcgagctgg ataagaccat taatacggat gtttgggttt
     6721 atcaggcatc gcacgtttac tccgccaccc atcgtttgtt gcggcatacc gatcaggcca
     6781 gtattggcat tcaacacaat ggcttcgtga atatatttat cctccggcac catctggacc
     6841 tgaccatcca ccagttgcca tgtcgctttg cactgcgcag caatattatc catcacgttg
     6901 cggctggatg agtaaatcgc gcggccacga ggaaacacgg tatcaggaaa atcgccggta
     6961 atgccctgtg tcacgccgaa cgcgttgaaa tcctgcatcg tcgcccggtg cagatccgca
     7021 acggtatatc cagcggcaag cgtggtgatg gtggtcgcat agaggaacgc ttcgtgatca
     7081 ccaatggcct gaatcaacac ccaggaatcc gtaatgttgt ccttcccggt gacggtgaag
     7141 cgaatatcac cgtcaaaaat caggccgtag ttctgaccgt tcacctgccc tatctggtct
     7201 ggtgaaatct cccgggacac accaacctgg ctcgcatcaa catccggcgc tataccgtca
     7261 tacccggcaa tgatgcgaat tttggcaaac tcctgcccaa gtatcttgtt cgtggtatcg
     7321 gtcgaaaggt tgtaaatttt cacgttcgcc acgcgcggcc agcgtgtgtc tgcccactcg
     7381 atctggaacg tgaccttaaa gtcagacagg gaaacgccct gcccgttctg gtccaacagt
     7441 tgcagctcaa aatggcgcat ccagttaaga gacatttcta ctcctgtacg aaaatgaggt
     7501 ggctgtatgt gccgaggttg gttttggtgg gctcgtctgg tgcgccctta tcggtcgcca
     7561 ccaccagcgc gccatcaatg ccaagctgtg gatattgtcg taaaaggttt acgccggtca
     7621 gtagaggtac gccagagaga agcgcggcac cgccgctatc catcacgtcc ataatccagc
     7681 cagccgcatc acgccagatg atcctgagtg tatacgtggt attgcccagc aaaactcgga
     7741 actgctgatt gtcaggagaa agcggtattt cgttaaactg catatcatcc cccgaatgct
     7801 gatgtaacgc ttccgcccag ctggctcagc aaggattcgt ttggcggtgt agtggatttc
     7861 atcccggaat tctgcaccgc cgatgtgctg acgccatcct gcatatctga tttatctgca
     7921 acgctaacgc tctgcgtctg cgacatgatc acttcacgca gggtaagcgt gcagttcagc
     7981 acgttctcgc tggttttatc cgttgtcacc tcgatggctc gcaccagcat attgctgtaa
     8041 acccgctttc cggtcactac atcgaacggc accctagagg actggagatc cagtagttgc
     8101 tgataggtct ctttcgggct aagtccggcg ctgaggccga ttgacgatgt atcaatgaag
     8161 tccagcaacg aaccgccacc tgcgaagccg cattccatcg tgacttcgct ggggcgctta
     8221 tacgcatgat cggcgatgaa acccgacgcg ctattcgttg ttggcttctc caccgggtgc
     8281 tcagtaattt cgagcgcatc agaatgcttt tcagaaacaa ccacgctggg gattaatatt
     8341 ccaattcgcc gggattgctg gcgaaaaatt gctgataaaa tatccattat ctcggtcctg
     8401 cggggagttg ctgggttaac tgtgaattca cgcccttttg acggtcaaca gtcaaacggg
     8461 cagcctcgcg cggatcgaaa acgccgtgga tgttaatatt cgtttcctgc tgaatcaccg
     8521 gggcgctggt gggcatattg ctcattactt tcggaatgta gttgcgcgtt tcctgcggca
     8581 ttagccccat tccataacgc ttaacattcc cgatccccca gttatatgat gccagtgctt
     8641 tgctaaggtc tccgccgttc tgccgcaaca gctggctcag gtacttagcg gctgcctgag
     8701 ctgacttttc cgggtcgaat acatcattcc cacgcaggcc catgtcgcgc gccgtgccat
     8761 ccataaactg aaacagacct ttagcgccag cgcctgaaac ggcgaactga ttaccgcccg
     8821 attccgttat ggccacgctg cgcaataacc cctccggaag ccggtagagc tgttccaggt
     8881 tggtaaacat cggctgcatc catcccagca gttcagagcc tgctttggtt ggctgtggcc
     8941 gcttaactga ctggccgagc tgctcaggct catcatcacc gaaccagccg cgcaccgttc
     9001 ggcccacgct gcgaggatcg aatccccagt gctctttaat ccagtcggcg gttccgttgg
     9061 cgctgtctgt taccatcggc atcgctgacg gattttcgct gccctgatta agcatctgtt
     9121 tgccgatgct ggcggcatca gcccagcggc catctttaat ggcgttgagc aggtcggcga
     9181 tcatgttcag catcttgccg aactcaccca tttgcgaaat gaagttgcta aaatcccact
     9241 ttagggacca tgacttgggg tcaataccga gcaacttagc cagcgctttt gtcaggtccg
     9301 taacagtctt tttcaggtca ccaatcattt tgacggcctg atcaatttca gtcttccatt
     9361 tcccccagtc aatcaggctt ttgccacctt ctttccacgt ctggtaatca tccaagagca
     9421 gaccaagtgc aataataagc gtcgttatca tgcccaaggg tgatgacatg aaagcggtat
     9481 ttagcagccg ccatgcaacc aacagcccac tgaacaatgc gataagctgc tgcgtggctg
     9541 ggttcagctt tttgaaccag gcgatcacac cctcaacagc ttgcccagtt cgccataaca
     9601 cgcgggtaat cgcgtcacct gcccagagaa ttcctttgat gatcttcgtg atgaccgctt
     9661 caatcttcgg ccagttgtcg agtatctgcc tgcggaagtt atcaatactg ccagcaaggc
     9721 caccagctag gttagagcca atcttgtctt tcgcctgtcc aagcgtcatc gtcagattac
     9781 gcatggaggt catgaaaata ttggactgtt tagccgctga ctcagcatta aaaccaatac
     9841 gctttgccgt caacgcgtac tcagaactga gctgccccat ccctctgcgc atcgccatca
     9901 gcgtgttttc atcgatgccg agcatctgcg cgtattgctt cgcgcggtaa tacggcatgt
     9961 tgttgagctt ttggccaacg ccagtaaaga tggcagcagt atcacgcatc tttccgctgg
    10021 catcgcgggt ctggacacca agacggttca ggaacccttc cgcccccgga ttgctacgca
    10081 tgaagccagc caacccttcg agggaggaca tagccgactc ggcgctggca ccggtttgcg
    10141 atgcggcgta tcccagcgct ttgatgccct ggacactggc ccccgtccgc tgggatgccc
    10201 agtaaatttt atccagaccg ttcgcgatct gagtggtaaa tccgacaatg ctcagcgctg
    10261 cgcctttcac caccgcgccg accttcagaa cgttcgcggt aacgcctttc agcacggctt
    10321 caaacttatt agcgccagcc tgatcgatat cgaatcccag cgaaacaagg aaatctttaa
    10381 tcgtatctgc gttaccgctc attggccgct ctccatttat ctacccgggc gtcgttatcc
    10441 tcgcgcatgt cgaggtagtc attgagaagc gcgatgcggc aaagatctac cgcaccgctg
    10501 ttaaggtctt tctggtcaat atggaaggca agcgccgggc gaagaataaa atcttcaccg
    10561 cccggcaggc tgttgaaggt tattccgcta gcggggtggg cgtctcgctg gtagggagtc
    10621 cttgcaaaaa atttcccagc gagtcggcga ccacccgcgc caccagttgc agcatggtaa
    10681 gcaggtcgat atcgtcaaac gccatttcgc catgctggca gaccggcacc cagcctttca
    10741 tgtgctcgcg tgaaacaacg gaaagacagg ggaacaggat agcgtccacg tcgccatcac
    10801 tcagatcgga cacagcattg gcaatctttg gcaggatagt agccatcgcg ccttcggtgt
    10861 ctttgctgct gatcttctcc tgaacgctcc ggaagtccga aaccatcccg gccagaaccg
    10921 gcaacagctt gcgggacacc ttcagctgtt cgaaaacgct gagctttgcg gtgcgatatt
    10981 tcacgccttt aatttcgaat tccatgcgtt aaaactcccc gagaagctgg tcaatcttgc
    11041 cgcagtcgaa tacccaggcg acggttccgc cctctttagc gttattgaaa tcaggctgtt
    11101 tctggaatgc acacgaacgc gcagtagaaa tatcacccga tgccgtgttg cgaatgacga
    11161 tcacgttatt gccccaggtg gcagaggact ggctttgcgc gttatacgcc agagacagct
    11221 ttttattcac cggggaggtt ttgagtagcg tcaccgtaat ggtgcctgac ttatcggcgt
    11281 gcaggctgtg catcacctcg ccatcggcac cgatagtcat ggtgtttttg ttgccgccca
    11341 tggtctgggt gataccttcc tcagagttcg cagaaccctg accaagatcg ataacgccgg
    11401 tcggcccggt gagcgacgcg gttacatcga gaaaagaata agttgccatt tatcgctcct
    11461 tagcgaacca cgttgatctg cacatcggca taatgaactg cgccagccag cttacaggcc
    11521 acctggatta acggtgcttt gcgcgcttca cggtcggcct gcgcctgttc ggacagcggt
    11581 tgcgcataca cgtaatagcc tttggtcagc gtatcgccgg aattcagctg tccgataggg
    11641 ccaccattcc acacgccagc agctaccaga ccgttcgtga cggactgatc catggactgt
    11701 tcaacgttgg aaagcagacg ggtcacaccg gcatcagtct gcggaatttt ggtggtgctg
    11761 gtgtaaagca ggttatagag gttggtctga acgtagttct gcaaccagtc gagcccgtgg
    11821 cgctcgtcga agaagtcacc gttcgccatg acaccctgtt gcaggatcgc cgtgtcgttg
    11881 gcgtagtaca cgaatacatt gcagtttttt gcatcaagtg ccgatgcctg gctgactgtc
    11941 agtgtttcat acccgacacc cggctcctgc ttaaacttga gcgtaatcgc ggtattactg
    12001 ccattgaaat taaccgtgaa tgcccggcca aatgcagata acgcagcgta tttattaccc
    12061 gatgaatatt gaataaaact gcgtgaatat ccggcggttt tcagttttga tgccaaatca
    12121 tcgctggatg cagtctgcag gcatttctca tcgcttgtcg taatcgccag aatacggctt
    12181 acagaagagg attcgatcgc cgcagccact ttcagccagt ctgcatccgg aatatcttca
    12241 tcgtctgcaa tccccagccc ataccatgaa gtataatcga gcatggcatt cacagcctgc
    12301 tccagcgtct caggcgtggc ctgttcgctg tctcccttcg ttttcaccca acgaccaaca
    12361 aaaacctcct gaggtttcgg tgattgtgag aaaaacacct gcgcagcctt atattctggt
    12421 gattccacgc caaaatcttt tccaatatct tccgcggcag aataacggcg aatgcgctca
    12481 cttaccggaa tgattgtgga cgggccgaga atgagtaatg caccaaaatt tcgccctgat
    12541 gctgcacgcg gcgacatgat cacatcaaca ttaacaacgt ttgatacagg caagccctgt
    12601 gccatagctt aatctccgaa aaagatgact ggtgcttcca ccagcgattt aataccgtac
    12661 tcgcgcacaa ccttccggcg cagacgcacc gtcatatcgt agcggcggac ccattgctga
    12721 ttaataagtt cagggaagag acctgtgtaa tcgccaagag acagccccag cgcattcagt
    12781 gctgcgttgt tctgcggtac agatataccg tcacgaaacc gggacgcata caccatcccc
    12841 gccggaccat aaaacgaagc catacactca atcgtttcat gccgccagag ctgagagcca
    12901 tcatcggtct gtctggtgaa tgccggactg tcatcacctg accatccgat aaccccaaac
    12961 gcacaccagt tcgtttcaac cggtagcagt ggcggctgct ctttctgcca gcgcgggcga
    13021 accatcccgg cagacagacc ggaaacgtta cgcatccact ggcttaacag cctgtcgagc
    13081 gcttcgtcat aatccggatc gccactggtt ggtattaacc atccgcgctc tgtactggtg
    13141 ttattgctca accggagttc ccccatcaaa cggcatcaac tcacaatgcg cctgaacgaa
    13201 tccggcccca taagctgtat acgggtcgac gaaggtcaca cgataatcac ggccctgata
    13261 cgtcacgata tcggcatcac ggccagtctg tccctgcgtc agtcgctcag tcgtcacaat
    13321 cagaattgca ccgctgatta cctgccctgc ctgcatacgg cggttttcca gagagcgatc
    13381 aacagttacg actccggcaa actgcttttt aacttcactg tcgctgccga tcccgtcctc
    13441 atccaccgtt tgcactcggc gtgttaccca caaattgaag tcgcaaaaat cggggtcaaa
    13501 aagcacatct gttacatcaa gagtcggcat ctttatccct cacaacatgg gtaatagctc
    13561 tgcgatattg cccggtgtca attaatggtt tcgccagatc ggttccggga gattcgccag
    13621 caacacgccg agcaagttcc agtgttgccc ccttgcgccc ccgacgagcc cgggcttcaa
    13681 cggtgctatc agcaagcggt gtaaagccgg taatggtcat gtaacgcctg acgccattcg
    13741 cggccagcgt tccggcgcgg ttaagcgctc tttccgcacc cgccgcattt ccatcaagcg
    13801 cagcctgcgc cgctgctttg agctgcggca ccgtctgttc ctctacggat ttaacgccgg
    13861 ggatcaggtg cgggcgtggg ggtatgtttt gcgctggtga gccgtattcg ttgacgtaac
    13921 cgatcccggc attaccaaac ggaacatcct cacgctcgct gtcttcttcc gggatgccca
    13981 ccagcacatc ctttttggtt agcgaccgga gcgcatccag aatggcctga gcgttatcaa
    14041 ccctcgttgt tacaccactt ttgaaactca tagctggcga ccgcccgcac cgaacatcgt
    14101 gatcagctga taaaattcag cgccatatcg ggtgttattc cagaagcctg cgtcagggtt
    14161 tagcgtcgcg ctggtgtcat agctgacgct taccttgtca acggacttgg aggactgaac
    14221 accattggtt gaaccgcccg ggccgccaac cagcattgcc cgactatctg ccgcccatag
    14281 cgtcatgtag tgagccacga acaacccggc aaagtacgga aacaaatctt tgccggtgac
    14341 gttttcgctc agcagttcat cggccagatt cagacgaaac tggatttgcg cttcgggata
    14401 tttggcaggg tcagcaaact gcgggaagtc gcggcgaaaa tcacttaccg ctggcaggct
    14461 ttgattcttt ggcatttttt acctcgttac gcgcgtctgt ggctttgcca acggatactt
    14521 ccgcgtgcgc acgagtgaac cagtgcgtgg caacgtcttc ctccactgca tgacggcctt
    14581 taacaaactc gcgccgcgaa ccgtcgggaa gcgtgagcac aaacggggta tgtacgtgta
    14641 ttactgcatt attttttgcc atcgggtcat ccttaatggc cccgccaggg gggccatgtg
    14701 gctgttaaat gccatcaacg tacgaaatgg tttctttgta cactggctca accgcaccca
    14761 gcttgccgta gtaggtcgca atctgataca gaccgcgata ctggatagga acgctctgca
    14821 acggcaccag cggatagcgg acgtattttt tatcgttggt gtacgcaacc atgcgatcct
    14881 tattccccac accacggcct ttcagccatt taaccgcgcg gatattcagc ggaacaccgt
    14941 tctggtgata gctgatggtg ttggtctgaa ggtacgtcaa cagggactgg ttacccgcag
    15001 atgaaacgat gatgctggac aacagagcaa actgctctgg cgggatcagc aaatcacgcg
    15061 gaaccacaga gtaaccggaa gcggcccacg catcagacag cacctggtta atgcttgcgc
    15121 ggatttcgtc cggtgttgag attgcccacg ttttggcagc gttgttgaca ggcacgccgt
    15181 ccagggtaac aaggcctttc aggtttaatg cggaatcgcc aacatacacc tgctcgtcgt
    15241 tgtccatctg ccatttgagc tgcatcccgt cgtacttctg ggtgtcaatc gggcgaccta
    15301 cctgctgagc tgctgccagc tctacaacgg tccagcccag ttccatgccc cagagattca
    15361 gcgggttacc ggatttgccg atatccacgt ttacgccagc aatagcggtt gagtctttgc
    15421 ctacccagtt tttgccattc ggatttgcac cagtacccgc agcggcgaag ctggtattcg
    15481 tccagctgga aatgtcatct gcgatagaga catcttcacg caactgaata tcgcgggtcc
    15541 aggtgtaccc cgccagtggc aggttcagag tctggtcgag tcgctccagc tctccgatga
    15601 gaaaggcacc agagctgtca acggttgcct gatcaaaagt aatcattcgt ctgttcctta
    15661 aatcttccag gaaatttctg cattgccgtt agcatcaccg gcacctgtga attcagcgtt
    15721 ggtcagcacc acatttttgc cactgactga cgtggacatg aatccaccca gcggcacttt
    15781 gatggattca tcagtggaga cgacaacgta taccgggtcg ccttttttga tggtgctggc
    15841 atcaaaatca gaaccgagat taacggtcac gtagccacgc ttcatggcgt cgcccgggaa
    15901 gttcttgcct gttcccacct ggcgaaccat gtccggctgc gaagtggtcg gataagggcg
    15961 cacgtagatc cccttcacct tgtctgcggt atcgccatct gccagcggca cgaaaaaacc
    16021 gtcagcatcg tatttaccag ccagcccata ggcagcaaag gcgttatcgg atttaaggac
    16081 caccggttcg acggttaagt cctgcgggcg agagatagcc ccggcaatgc caacaggcat
    16141 ccggtacaga tatgcagtca ttggattatc ctttgcggtt agaccagaat tcagcgtttt
    16201 gtttgttcag ggaagcgatg ctggtcatgc ccatgtttgg acgttgtgca tcgcccgtgg
    16261 tgctgcgggt gtttcgccct ttggcaatct cagacacagc gttaaacgcc atatcgaccg
    16321 attgcttagg caatttgcgg atatccgcat caccgacaac ctggcgaacc agcgttttgt
    16381 cagcagaagc cagaacctcg cgtttgaaca cggtcggttt catcttacgg ctcagatcga
    16441 tacccggaac gataacttcg gcacgccagg ctgagtcacc agtaatcgtg gtttcctctt
    16501 catcgtcctc gccgtcaccg gtcggattat cgtcaggttt attatcgtta tcgcccgtgg
    16561 catttccttc cagcttagcc agcagggctt tcagtaatgt tttgaggtca tcatcactgt
    16621 cgccggttgg acctccgccc atctctggta ctttgtccgg tagcggttgc tgcggggaca
    16681 ggttgatgtt gagattaacg ccctgcggca aatccccctc atctcctgta accgatgcgg
    16741 gagccgactc caccagttcg ttcatggtgt cagcgtcacc cgttttgatg gccgtgcgca
    16801 tgcgggtcca ccagcttttc ttttgatttg ccattgtgtc tctgtctcca attgcacaac
    16861 gatttccggc tctgcctttg gggacaagag ccacatggtt tccggtaata tcgacctgct
    16921 cggctttacc tggctcggtc tgctcgtact ccgcgtcata gccgcacgac acttcacgca
    16981 ggccatcttc gataagctga atggcgtttt cgtctttgac gataaggtca gccagcatca
    17041 aatcagagtg gtcacccgtc ccgcgccgca cattctgaag atgcccgaca gcaagctctt
    17101 tccagttctc gggatttacc agccgcacat tcccgttttc atcttcagga tgcaggatcg
    17161 tgatgctcat cccttcgaat gaggcgagcg tggccggatg gaatacctgc tcaggagagc
    17221 gcgttacgac tatctcaccg agcttgtcgg gtttgaggtt tggcagatcg gcagcgccgt
    17281 agagctgttt acccgttcga cctatcggca cgtctttgca cagcagcgag ccgtcagcca
    17341 gctgatagcg ggtttccccc agccgggtat tgaaaaaata tttcatggtt tacctgcgat
    17401 tcaggcgaga taagaatgag ggttgggaaa aacgatttct ttataacagc gacaattcgg
    17461 gagctcgcca gcgtgaccgg tcatgccatc aagcgttgga ggtttgcccc attcgacaaa
    17521 cttaccttcc atctcccgat gagaatgccg gacgtcgcca tcttcggctg tacgccagat
    17581 ataaccattc gagccgattg acagcgcacg cgcctgatcg agcgcgccgg ttgcacgtcc
    17641 aagctcggta cgggcgataa ggttcgctcg tgagcgtgac acgtcaccgg acgctgctat
    17701 ctctttcgcg aatggttcag cgcggccacc agccacaacg gcctcgatgg ccttgttctg
    17761 aatgtcatac acccgatcgg cggcctcaag aggcagtgac ttgatatact taatttgctc
    17821 ggcgacgatg gatttcatca cctggcctac cggggcgcgg tcgaccatgt tgcgcagctc
    17881 tgcgctgatg ttccggctgt gctgacgcca ctgcttttca ttctggcgtg caatgtcggc
    17941 ggtaaagttc tcagcaacct tcgtcgccca gggggtgatg atttcgctgt agcgctccag
    18001 cgcatccatt atttcggtaa cgctatcatt tgaaccatcg tagcgcccat ttacgatatc
    18061 cccgaccgcc cgcgctatct gccgtaggct cgttcgatat cggatctccg cctggcggct
    18121 ctggcggttt gtcgccaagt tcgccgatgc ctggcggcgc ttcgtcttcg gcattctcga
    18181 tatcctcgtc ggtaatggat gccccgatgc cggtgacgtc agagttttcg cgcaggtcgg
    18241 tcatcgccgc cttacgcgtc atcaatccgt cgcccagcgc ggtactaatc gcgttggtgg
    18301 tgtttacggc caccgttgat cggtcaacgt ctgacatttg ccatagcggg ttaaactcaa
    18361 acgtgaaatc gtccggcagc ggctttccga gttccgagcg gtgcataatg tccagtatcc
    18421 ggcgcatcgg cagccgtaag cggcgctcct gcaatgagct cacccggtcg taatagttgg
    18481 cgaggtctgc atcaccagta gagaagcctt tcggggattg accgaacaac cgcaccagcg
    18541 ggataccaac ggcaccgcta atctgctcag caaactgcga aagaatgtca tccagaccac
    18601 taaagctgta ctggtgggtt tcgaacttat cccgcgagtc catgagcgtc ataccttcat
    18661 tgctctggaa ctggcggatc aggtcgatgt tcttcagcaa cgcctcgaac gccgggcctc
    18721 caagcgcgat aagctcgcgc aacttctcca cgctataggt acgcagatgt gctttgtaga
    18781 ccagctgcgc cgcgccgaca gtggcgctgt cgaacgcagt aagccgatcc cagatacgct
    18841 ctacaaccga cattccccat tcgttttcgg tcatcttctg ctggaatggc agcgtcaccc
    18901 cgtcgaagcg aatcaggcgg ctgtgatgga tgcgccaggc cgggatgccc gttgcagtgg
    18961 tcaccacgtc gtaaaactca ggtttgccga gatctggccc catctcttta atgcggcggg
    19021 tcaggaccgg gttaatcatc cagcggtcga gcgggagaat gcccttaaac ttgccttctc
    19081 caatggtttc gagccgcagc ggggtcattg gtgcctgccc ctcgatcatg atgaagccga
    19141 ccgcgccgcc gtagaggcga gaccatttca gcacgtcgtt cagcgcatcc cagatttgca
    19201 actcatccag ttgtgattcg agaatgccac gatcttttgc atcaatttcc gaagtgatgc
    19261 gaatgccttt gcgggtcata tcatccggga tagcatcgac cgcttcgccg ataatccagg
    19321 acgaacgata ggaccattcc accagcatgc ggttacgact ggtgaaatta gcccggtagg
    19381 tggatgctga gtgctggtta ggtgtctgca tccccacgcg ggcaacaaag ttctcatagc
    19441 catcagcggt ggcctgtgcc gttcgccgcg tggcttgttt gtttcgtgcc atcaggcctg
    19501 tctccctagc agctcccaga tgttcagggc tgaattcatt ggcgcgtagc tgatcatcac
    19561 cgagtcggca aggtttggcg accgggttcc atcaggctgt ttatcaataa cgatttttcc
    19621 cacaccatta atggaatagg tcggctgcga aagctcgatg atgagtttat ctttgagtgc
    19681 catgctactg ctgattgaga tgatttcgtc cgggttgtaa tccatacctt caaccacggc
    19741 gcgccaggta ttctgaaaaa gtttacgtaa ccgccaccag ctctgggctt tggcgttagc
    19801 gaagaagtcc ttgttcaggc gggcggcctg tccgttgtca ccgcgcaccg cttcgtcgtc
    19861 cggatcaaat accgcgccac tacctcgaaa cggtgtggcg agtattgacg gtcggcgcgc
    19921 agcgttacgc agttcgttga tagcgcgtgc atcgccgcga acgccagcgc ccaacccgtc
    19981 ctcgtcaaag cgaaactctt cgaggttgtc ctgttcgcaa aagccgaaaa ccttctcgac
    20041 ggactgataa atgtcgctgc ccacaccgga ccattcccgc acattctcca ggaggaagcc
    20101 atgacgggtg gaaaaggaat ttttgtccct gccttcgtcg gcgacgtcca tcgcgcccag
    20161 tcgcttgcct gttggctgga tgcccagctt gatatgcgcg tcgacagcag cctgtaccca
    20221 ttcggatgga atcaggacgc cttccgctga tgcactgtag ttcaggtcaa gttcctgtgc
    20281 caccaccacc ggattgtcga ttttctcgca ttccctgcga taccactctt catccttgcg
    20341 aggatcatcc cgccagtgga atgtgaatac cggtatcttc ccgccatgac gcttctgagc
    20401 gaacgggttc gccatgccgt taactgaact caggtcaata cggcaacgcg tcgtttgtga
    20461 caacgccgca tcaatcagca gaggacgctg aaggaatgca gactcatcaa ccagataaag
    20521 cgtggtacgg tcaccacggc caatattatc gccagcctca cctttgataa cggcaccagt
    20581 ttcaggaaac tcaacgcgca tgtatggtgc gtgcttcttt tcgtcccacg aacctcgaaa
    20641 ctctacaggt agtgtttcca cgaacttgcg cgccttccag aacaatgctt tcgggtcacc
    20701 ggtgctgtcg acgtattcct ctttacggga gccgaaaccg ataaccattt ctttgttgaa
    20761 gagacaaagc gagcatgcca gcccgatcgc ggtccaactg agccccattt cacgggattt
    20821 ttcggtaata ccattctccc gattacccca gcgttccata atccagtgga tccactcctc
    20881 ctgcttaggg aagagtaaaa acggaatggt caccggcagg ccataatcaa tattacgcgg
    20941 gtccgttgtc atgccccagt cgatgatgaa ctgagccgga ttggttcggt aaaactgttt
    21001 tagtgcaggc aatatttcag gattctggcg aatgcgctgt aggcgttcca tccgccattc
    21061 aaaaaccatc tggtaatcag gatgtttaaa atcgaagggg aatggtaacg gcatacttag
    21121 cccatcattt ttctatacgc ctctgcagcc tgctccggcg ttaagttggt aatttctgtt
    21181 ctgactggtc ctccgtcagc accagtcact tcatttttga cattgtcttt aaacgcctga
    21241 acagaaacat gacgcccgag caactcaagg tttttaacct tatcaggcca tttgattttc
    21301 ttcaggagag cagcactatc tgctgatgcc atctcgacga catccattcc tgatagcgtt
    21361 gtacgccaca ccttaggcca gtctttaatc tgcttcagtt cgccattggc aagcagaatg
    21421 tcaagcacgt ccatctgatc gatttcagtc agccgtcgca atacataggc ggcgtctata
    21481 cctacctctt cattgcgttc agcctttagc tcagcaatgc gatctgctat gtcaggtttt
    21541 gacaggttct cgctaccagt ggcgcgagca gttttctcgc tgtaacccgc cctgatagct
    21601 gcctgagtgg cgtttaaatc ttttaaatat tccctgcaga acagttcctg cttaggtgtg
    21661 agctgtttca aatcacacct ccgaaacatt gctccaattt c
//
LOCUS       DAEEUE010000064        21505 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.064, whole genome shotgun sequence.
ACCESSION   DAEEUE010000064 DAEEUE010000000
VERSION     DAEEUE010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21505
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.064"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(37..2106)
                     /gene="glyS"
                     /locus_tag="KLK84_002612"
     CDS             complement(37..2106)
                     /gene="glyS"
                     /locus_tag="KLK84_002612"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBE2009886.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2116..3027)
                     /gene="glyQ"
                     /locus_tag="KLK84_002613"
     CDS             complement(2116..3027)
                     /gene="glyQ"
                     /locus_tag="KLK84_002613"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBE2009887.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVGF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3122..3421)
                     /locus_tag="KLK84_002614"
     CDS             complement(3122..3421)
                     /locus_tag="KLK84_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HBE2009888.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3596..4591
                     /gene="wecH"
                     /locus_tag="KLK84_002615"
     CDS             3596..4591
                     /gene="wecH"
                     /locus_tag="KLK84_002615"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HBE2009889.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4633..5070)
                     /locus_tag="KLK84_002616"
     CDS             complement(4633..5070)
                     /locus_tag="KLK84_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HBE2009890.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5116..5457)
                     /locus_tag="KLK84_002617"
     CDS             complement(5116..5457)
                     /locus_tag="KLK84_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009891.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5626..7080)
                     /gene="xylB"
                     /locus_tag="KLK84_002618"
     CDS             complement(5626..7080)
                     /gene="xylB"
                     /locus_tag="KLK84_002618"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBE2009892.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7152..8474)
                     /gene="xylA"
                     /locus_tag="KLK84_002619"
                     /pseudo
     CDS             complement(7152..8474)
                     /gene="xylA"
                     /locus_tag="KLK84_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8840..9832
                     /gene="xylF"
                     /locus_tag="KLK84_002620"
     CDS             8840..9832
                     /gene="xylF"
                     /locus_tag="KLK84_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HBE2009893.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9910..11451
                     /gene="xylG"
                     /locus_tag="KLK84_002621"
     CDS             9910..11451
                     /gene="xylG"
                     /locus_tag="KLK84_002621"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HBE2009894.1"
                     /translation="MSYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11429..12610
                     /gene="xylH"
                     /locus_tag="KLK84_002622"
     CDS             11429..12610
                     /gene="xylH"
                     /locus_tag="KLK84_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HBE2009895.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12688..13865
                     /gene="xylR"
                     /locus_tag="KLK84_002623"
                     /pseudo
     CDS             12688..13865
                     /gene="xylR"
                     /locus_tag="KLK84_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
     gene            complement(13973..14797)
                     /locus_tag="KLK84_002624"
     CDS             complement(13973..14797)
                     /locus_tag="KLK84_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HBE2009896.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15117..17147
                     /gene="malS"
                     /locus_tag="KLK84_002625"
     CDS             15117..17147
                     /gene="malS"
                     /locus_tag="KLK84_002625"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HBE2009897.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17325..18578
                     /gene="avtA"
                     /locus_tag="KLK84_002626"
     CDS             17325..18578
                     /gene="avtA"
                     /locus_tag="KLK84_002626"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HBE2009898.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18729..19202)
                     /locus_tag="KLK84_002627"
     CDS             complement(18729..19202)
                     /locus_tag="KLK84_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBE2009899.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="KLK84_002628"
     CDS             complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="KLK84_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HBE2009900.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLSAIT"
     gene            20353..21351
                     /gene="yiaK"
                     /locus_tag="KLK84_002629"
     CDS             20353..21351
                     /gene="yiaK"
                     /locus_tag="KLK84_002629"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HBE2009901.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21363..>21505
                     /locus_tag="KLK84_002630"
     CDS             21363..>21505
                     /locus_tag="KLK84_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBE2009902.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYA"
BASE COUNT         5399 a         5489 c         5283 g         5334 t
ORIGIN      
        1 gcctgccaga tggcaggctt tttaataacg gcgttattac tgcaacagcg aaatatccgc
       61 aacgcgcagg aacagttcgc gcagtttctc cagcatggtc agacggttga gacgcaattc
      121 tttgtcatca accatgacca tcactttatc gaagaaagca tcaaccggct cacgcagctc
      181 tgccagttcg accagcgcat cctggtaacg accttccgca aagtacggct ccagcttgtc
      241 acgtagcacc acaacctgca tcgccagttt aatttcttcc ggctctttca gggtagaggc
      301 attcacgcga tcgctcagca cttcgtcaga tttcgccagg atgttagata cacgtttgtt
      361 cgccgccgcc agtgctgcag ctgcttccag ggtacggaag tgcgataccg ctttcatgcg
      421 ggcatcgaaa tcagccggac gagtcggacg acgcgccagt accgcctgga tggtgtcaac
      481 ggtataacct tcgtcctgat accaggcgcg gaagcgaccg agcataaagt cgataacatc
      541 atcaactacg ttggcattag tcagcttatc gccatacaga cgcaccgctt cttcggtcag
      601 cgtttgcaga tcaaggttga ggttcttctc aacgataatt cgcagcacgc caagtgcggc
      661 acgacgcagc gcaaacgggt ctttgtcgcc tttcggatgc tgaccgatac cgaagatacc
      721 cgccagagta tccatcttat cagcaatcgc cagcgcacaa gctaccgggt tggacggcag
      781 gtcatcaccg gcaaagcgcg gctgatactg ctcattcagc gcgacggcta cgtcttccgc
      841 ttcgccatcg tgacgcgcgt agtgcatccc cataacgcct tgggtgtcgg tgaactcgaa
      901 gaccatgttg gtcatcaggt cacacttaga cagcaggccc gcacgggttg cgtggttaac
      961 gtcagcgcca atctgttcgg cgatccagcc agccagcgcc tggatgcggt cagtcttgtc
     1021 acgcagcgta cccagctgtt gctggaacag cacggtttgc aggcgcggca ggttatcttc
     1081 cagacgtttt ttgcggtcgg tgttgaagaa gaactcggca tccgccagac gtggacgaac
     1141 gactttctcg ttaccagaga taatttgctg cggatctttc gattcgatgt tggcaacgaa
     1201 gataaagttc ggcagcagtt tgccgtcgtt cgcatacacc gggaagtact tctggtcacc
     1261 tttcatggtg taaaccaggg cttcagccgg caccgcgagg aatttctctt cgaattttgc
     1321 ggtcagaacg accggccact ccaccagcga agccacttct tccagcaggc tttcgcttaa
     1381 gtcagcgtta ccgccaatct tacgcgctgc ttcttcggca tcggctttaa tcttcgcttt
     1441 acgttcttcg taatcggcga tgactttccc gcgctcacgc agaatttccg gatactgatc
     1501 ggcgttgtcg atggtgaatt ccggttcgcc cataaagcgg tggccgcgaa tcacgcgatc
     1561 ggactgaatg cccagaatgg ttgccggaat gactttgtcg cccagcagca gggtcacggt
     1621 gtgcaccgga cgcacgaagt gcacgtcgct tgcaccccag cgcatcagtt tcgggatcgg
     1681 cagtttcgcc agcgaagtcg caaccatatt cggcagcagt gcttcggtgc tttcgccctt
     1741 cacatgggcg cgatacagca gccattcgcc tttatcggta gtcagacgct cagcctggtc
     1801 aacggtaata ccacaaccac gcgcccaacc ttctgccgct ttgctcggtt ttccttcagc
     1861 gtcgaacgcc tgggcaatcg ccgggccgcg tttttcgatt tcacgatctg gttgtgcttc
     1921 cgccaggtta gctactttca gcgccagacg acgcggagca gcaaaccatt gaacggtgcc
     1981 gtgtgcgagg cctgcgttat ccagctccgc agtaaagttc gcagcaaagg actctgccag
     2041 gctgcgcagt gcttttggtg gcagctcttc agtgccgatt tccaccagaa aagttttctc
     2101 agacatagcc gcctcttact tatctttgtt gcacatcggg aagccgaggg cttcacggga
     2161 agcgtaatat gcttctgcca ctgctttggt cagggtgcga atgcgcagaa tgtagcgctg
     2221 acgctcggtg acggagatgg ctttacgcgc atccagcagg ttgaagctgt gggcggcttt
     2281 cagaatacgc tcgtaggctg gcagcggcag cggattttcc agcgccagca gctgctgcgc
     2341 ttctttctcg tactgctcaa agcaggtgaa caggaagccc acatccgcgt attcgaagtt
     2401 gtaagtggac tgctccactt cgttctgatg gaacacgtcg ccgtaggtgg ttttacccag
     2461 cgggccgtcg ctccagacta ggtcgtaaac gctgtctacg ccctgaatgt acatggccag
     2521 acgttccaga ccgtaggtga tctcgccggt aaccggttta cactccagac caccaacctg
     2581 ctggaagtaa gtgaactgcg tcacttccat gccgtttagc cacacttccc agcccagtcc
     2641 ccaggccccc agagtcgggt tttcccagtt atcttccacg aaacggatgt cgtgaatagt
     2701 cgggtccatg cccagctctt tcagagaacc gaggtacagc tcctgaatat tgtccggcga
     2761 tggcttaatg accacctgga actgatagta gtgctgtaaa cggttggggt tttcgccgta
     2821 gcgaccatcg gtcgggcgac gagaaggctg aacataagca gccgccatcg gttctggccc
     2881 cagcgcgcgc agacaggtca ttgggtgaga ggttcccgcg ccgacttcca tgtccaatgg
     2941 ttgaacaatg gtgcagccct ggcgagccca gtaatcctgt aaggtcagga tcaagccctg
     3001 gaaggtcctg gtatcaaact tttgcatatt atttcgtgct ggatacgtgt ggatttaaag
     3061 gaagggatca gtatacccgc tggatggaag atatacagta cgaaacggga aaaagcaggg
     3121 cttaacgcat ggaaagatgc aaaaaatggc catctgcgtc aaaagaacag acaaactgct
     3181 ctttacggaa ggtataaccg cgcatttcat agcttccctg gaactgttcg aaggcggtga
     3241 cgtcgatttt ttgctcgccg gtgttataac gttttgccgc ctgatccttg cataacgctt
     3301 ccatattcaa cgaacgcaga ggatcaacct ttacccgttg tgctttctgc acgggtgacg
     3361 gtgtagaaca acccactagc accataagcg ccattgccgg aaagaaagcg ttcatcatca
     3421 ttaaattacc gtctggtttg caggtgagtc ttattattta tatcggtata gtggctgata
     3481 tcttacgaat ttccttaagc ctctataggc tgtagtgata aaactcagat tattccagtg
     3541 cgccaaccaa atgatttatc ggtcagactt tttttaacca gatacagaga cacagatgca
     3601 gcccaaaatt tactggattg ataacctgcg agggatagcg tgtttaatgg tggtgatgat
     3661 tcacaccact acctggtatg tgaccaatgc tcatagtgtt agccccgtca catgggatat
     3721 cgccaatgtt ctgaattctg cctctcgtgt cagcgtgccg ctatttttca tgatttccgg
     3781 ctatctcttt tttggcgaac gcagcgccca gccgcgccat ttcttgcgta tcggcttatg
     3841 tctgattttt tatagcgcaa tcgcactgct ctacattgcg ctgtttacct ccatcaatat
     3901 ggagttagcg ctgaaaaacc tgctgcaaaa gccagtgttt taccacttgt ggtttttctt
     3961 cgcgattgcg gtgatttatc tggtttcacc gctgattcag gtgaagaacg tcggcggaaa
     4021 aatgttgctg gtgctaatgg tggtgattgg cattatcgct aacccaaaca cagtgccgca
     4081 gaaaattgac ggttttgaat ggctgccaat taacttatat atcaatggcg atacttttta
     4141 ctacattctg tatggcatgt tgggccgcgc tatagggatg atggacacac agcataaagc
     4201 actgtcgtgg gtgagcgccg cgctgtttgc gacgggggtt tttattatct ctcgcgggac
     4261 attatatgaa ttgcagtggc gcggaaattt tgccgatacc tggtatcttt actgtgggcc
     4321 gatggttttt atctgcgcaa tcgcgctatt gactctggtt aaaaacacgc tggatacgcg
     4381 taccattcgc ggacttggct taatctcccg ccattcattg ggtatatacg gattccacgc
     4441 cttgattatc catgcgctgc gcacccgggg aattgagctt aaaaattggc caatactgga
     4501 tattatttgg atcttttgcg cgacgttggc agcgagtttg ttactttcta tgctggtaca
     4561 acgaatcgac agaaacagat tagtgagtta agtaaaagcc cgttcacatt ggactgaccg
     4621 ggcttacgtg aattattcgc tgtattcttc ctgggtaacc tgtgtttctt ttggcgggtt
     4681 tattccggca tcacgaatat tcttctgcac cgctactgca ccaaacaagc ttaagaagaa
     4741 agccagtcca taaaaacctt tttcactgag taataatgtc gcattccaca gacctaccat
     4801 cagtagagca acagagataa taaagacagt caggcaggtc atataataaa tggaagtggt
     4861 cggtatgcct tcatacttgt cccgaacggt cttttgataa gacgcagcag aaaacagtcc
     4921 taacaccagt acggcaaaat aatacccttt ttcatttaac tgcatctctg cattccatag
     4981 ccctaacagg taggtaacga taccaccaac gagagctatc catgacacaa tactaaaggc
     5041 cggtgaatag gttgatattt tgttgtccat cacagtattc ctttcattcc tgaatgtgta
     5101 agagctttat gttgctcata ccgagcgaaa caaacgcatt tgaccaaaca agcagacaaa
     5161 aaaggcgatg gggtagatca ccatttcata agcattgata ggtgcattta aaacaccgat
     5221 aaacaagaga ccgatagtga ttaacgcaac taactggcag atatgggtaa aaaaattatc
     5281 cagttgcccg agtttttctg cgcgaagata tgcatagtta ccaaaagttg ctgtcattaa
     5341 cacgccaaga aaatagcctt ttccacttag caatgggcat gagatccata gcccaaccag
     5401 ataaaccagc gccccgacaa caaataatag ttttgccaca gtttttgagg ttttcataac
     5461 gatctccata tctaccagcg atacattacg agtaaccaac gaaagacaaa actgaaaaat
     5521 gccattaaca aatgattttc agaataaatt catactaaat attaattaat tagtgagata
     5581 tatagatgtg aattatcccc cacccggtca ggcaggggat aacgtttacg ccattaatgg
     5641 cagaagttgc tgatagaggc gacggaacgt ttcacgtcgt ggctgataag cggcataacg
     5701 ctgcgcatct ggtagatgcg actgttctaa cggtagctgc ggcaacaact caatgagcga
     5761 tttctctgga ttcgccgcaa tctgcgccag ccttgctgcg cccagtgctg gccccacatc
     5821 cccccccgta cggtaatcga gctgctgacc gctgatatcc gccagcatct gacgccagta
     5881 ctcactacgc gccccgccac caatcaacgt aacactttgc ggtttaatgc cgcaggcatg
     5941 cacgacatcc atgccatctg ccagcgcata acccacgcct tccagcactg ctcgcgccag
     6001 ttcattgggg ccatgttgat gagtcaaacc aaagaaaacc cccttcgcct ggggattatt
     6061 gtgtggtgta cgctcgccgg aaagataagg cagaaaccaa actggctcgg cactttcatc
     6121 agcctgttga gctgcagcga ttaaagctgg gacattgcac aggccggtta atttcgcggc
     6181 ccaatccaga cacgatgctg cactcagcat tacagacatt aaatgccaac gctgcggtag
     6241 cgcatggcaa aagctgtgta cagcgctttc tggcttgctt aagaaccctt cgctgacagc
     6301 aaaatagacc cccgacgtcc ccagcgataa cattgcctga ttagcatcaa ccattcccac
     6361 accaactgca ccagctgcat tgtcgccacc gcctgcgaca actggcaccg tcgccatacc
     6421 ccacgctttc gcaacttcag gtaacaaagc accagtaatt tcgctgcctt cgtataatgc
     6481 gggcatctgg tcacgagata agtcgcaagc ctgcagcatg acgtcactcc agtcacgctt
     6541 tgcgacatcc agccacatgg tgccagctgc gtcagacata tcgctggcaa actcccccgt
     6601 catacgcaga cgcaagtaat ctttcggtaa taatactttg tcgatttgac ggaatatctc
     6661 cggctcatgc cgctgaaccc atagcaattt aggcgcagta aatccgggca tcatcaggtt
     6721 gccggtaatc actcgtgatt gcggaactct cgcttccagc aaagtgcact cttgcgcaca
     6781 gcgcccgtcg ttccacaaaa tggcagggcg caatacccgt tgttgagcat ccagtaaggt
     6841 tgctccatgc atctggccgg caatacccaa tgctttaacg tcctgcagag aatgctgatc
     6901 gcccagagct ttcattgcgc gatcagttgc ctgccaccac tgttccgggt cttgttccga
     6961 ccaaagtgga tgcgggcgcg aaacggtcag cttttccgtt tgcgaagcaa ccacctcacc
     7021 ctgctcgttg agcaaaataa cttttacgcc cgaggtgcca agatctatcc cgatatacat
     7081 atcgatcgtt ccttaaaaaa atgcccggta tcgctaccga taaccgggcc aacggactgc
     7141 acagttagcc gttatttgtc gaacagataa tgatttacca gattttccag ttgctcctgg
     7201 cgaccactct gatgcaccgg agacaaatta tgttcctgag catatttggc taaatctgcc
     7261 agtgacattt ggcctttcag gatttgctgg cccaattcgc tattccagcc ggaataacgc
     7321 tgcgcgatgc gtttatccag ctcgccatct tcaatcatgc gcgctgcaat tttcagcgcc
     7381 agtgccatcg tatccatcgc gccgatatga ccgtaaaaca gatcatattt atcagtactt
     7441 tgacgacgta ctttggcatc gaagttcaga ccaccggtgg tgaaaccgcc tgctttgaga
     7501 atttcataca tcaccagcgc attctcttcc acactgttcg ggaactggtc ggtgtcccag
     7561 cccagttgcg catcgccacg gttggcgtcg acagaaccga acaggccaag cgcaatggcg
     7621 gtggctattt catgatggaa agagtgacct gccagcgtcg cgtggttagc ttcaatgttc
     7681 agtttaatct ctttttccag accaaactgt ttcaggaagc catagaccgt cgcggcatcg
     7741 taatcatatt gatgtttagt cggttcttgc ggtttcggtt cgataagcaa cgtgccctag
     7801 aagccgattt tatgtttatg ctcaaccacc atctgcataa agcggcccag ttgttcacgc
     7861 tcctgacgca agtcggtatt taacagcgtt tcgtaacctt cacgaccacc ccacaggaca
     7921 tagttttcac cgcccaattt atgggttgct tccatcgctg taacaacttg cgttgccgcc
     7981 cagctgaaga cttcaggatc tgggttcgtc gccgcacccg cgccatagcg agggtttgta
     8041 aagcagttag cggttcccca cagcagcttc acgccgctct cttcttgctt ggctgccagg
     8101 acatcaacca tttgcgcaaa attattgatg tactctttta acgacgcgcc ctcaggggaa
     8161 acatccacat cgtggaagca ataaaatggc acatgtaact tgtggaaaaa ctcaaatgcg
     8221 acatctgctt tacgcttcgc caacgccagt gcctcaccag gctgctgcca cggacgatta
     8281 aacgccccca caccaaacat gtccgccccg ttccagcaga aggtgtgcca gtagcaggcg
     8341 gcaaaacgca agtgctcttc catacgctta cccaacacca gttcgtcggg attgtagtga
     8401 cggaatgcta acgggtttga ggattttgag ccttcataac gaacgcgatc gagctggtca
     8461 aaataggctt gcatattgaa ctccataatc aggtaatgcc gcgggtgatg gatgatgtcg
     8521 taatattggg cactcccttt caattgctca attatgttat ttcacactgc tattgagata
     8581 attcacaagt gtgcgctcgc tcgcaaaata aaatgggatg atgaaactgg gtaattcctc
     8641 gaagataaaa atgcaataag tacaattgcg caacaaaagt aagatctcgg tcataaatca
     8701 agaaataaac caaaaatcgt aatcgaaaga taaaaatctg taattgtttt cccctgttta
     8761 gttgctaaaa attggttacg tttatcgcgg tgattgttac ttattaaagc tgtcctctaa
     8821 ctacagaagg ccctacacca tgaaaataaa gaacattcta ctcacccttt gcacctcact
     8881 tctgcttacc aacgttgcgg cacacgccaa agaagtcaaa ataggtatgg cgattgatga
     8941 tctccgtctt gaacgctggc aaaaagatcg agatattttt gtgaaaaagg cagaatctct
     9001 cggtgcgaaa gtatttgtac agtctgcaaa tggcaatgaa gaaacacaaa tgtcgcagat
     9061 tgaaaacatg attaaccggg gtgtcgatgt tcttgtcatt attccgtata acggtcaggt
     9121 attaagtaac gttgtaaaaa aagccaaaca agaaggtatt aaagtattag cttacgaccg
     9181 tatgattaac gatgcggata tcgattttta tatttctttc gataacgaaa aagtcggtga
     9241 actgcaggca aaagccttgg tcgatattgt tccgcaaggt aattacttcc tgatgggcgg
     9301 ctcgccggta gataacaacg ccaagctgtt ccgcgccgga caaatgaaag tgttaaaacc
     9361 ttacgttgat tctggaaaaa ttaaagtcgt tggtgaccaa tgggttgatg gctggttacc
     9421 ggaaaacgca ttgaaaatta tggaaaacgc gctaaccgcc aataataaca aaattgatgc
     9481 tgtagttgcc tcaaacgatg ccaccgcagg tggggcaatt caggcattaa gcgcgcaagg
     9541 tttatcaggg aaagtagcaa tttccggcca ggatgcggat ctcgcaggta ttaaacgtat
     9601 tgctgccggt acgcaaacta tgacggtgta taaacctatt acattgctgg caaatactgc
     9661 cgcagaaatt gccgttgaat tgggcaatgg tcaggagcca aaagcagata cctcactgaa
     9721 taatggcctg aaagatgtcc cctcccgcct cctgacaccg atcgatgtga ataaaaacaa
     9781 catcaaagat acggtaatta aagacggatt ccacaaagag agcgagctgt aagcgttacg
     9841 ccccggcgcg gagcgggggc gtgatttctc tccatgccgc gtgaatgaat tggcttaggt
     9901 ggagtcgtta tgtcttatct acttgaaatg aagaacatta ccaaaacctt cggcagtgtg
     9961 aaggcgattg ataacgtcag cttgcggttg aatgctggcg aaatcgtctc actctgtggg
    10021 gaaaatgggt ctggtaaatc aacgctgatg aaagtgctgt gtggtattta tccccatggc
    10081 tcctacgaag gcgaaattat ttttgcggga gaagagattc aggcgagtca catccgcgat
    10141 accgaacgca aaggtatcgc catcattcac caggaattgg ccctggtgaa agaattgacc
    10201 gtgctggaaa atatcttcct gggtaacgaa ataacccaca atggcattat ggattatgac
    10261 ctgatgacgc tacgctatca gaagctgctc gcacaggtca gtttatccat ttcacctgat
    10321 acccgcgttg gcgatttagg gcttgggcaa caacaactgg ttgaaattgc caaggcactt
    10381 aataaacagg tgcgcttgtt aattctcgat gaaccgacag cctcattaac tgagcaggaa
    10441 acgtcggttt tactggatat tattcgcgat ctacaacagc acggtatcgc ctgtatttat
    10501 atttcgcaca aactcaacga agtcaaagcg atttccgata cgatttgcgt tattcgcgac
    10561 ggacagcaca ttggtacgcg tgatgctgcc ggaatgagtg aagacgatat tatcaccatg
    10621 atggtcgggc gagagttaac cgcgatttac cctaatgaac cacataccac cggagatgaa
    10681 atattacgta ttgaacatct gacggcatgg catccggtca atcgtcatat taaacgagtt
    10741 aatgatgtct cgttttccct gaaacgtggc gaaatactgg gtattgccgg actcgttggt
    10801 gccggacgta ccgagaccat tcagtgcctg tttggcgtgt ggcccggaca atgggaagga
    10861 aaaatttata ttgatggcaa acaggtagat attcgtaact gtcagcaagc catcgcccag
    10921 gggattgcga tggtacccga agacagaaag cgcgacggca tcgttccggt aatggcggtt
    10981 ggtaaaaata ttaccctcgc cgcactcaat aaatttaccg gtggcattag ccagcttgat
    11041 gacgcggcag agcaaaaatg tattctggaa tcaattcagc aactcaaagt taaaacgtcg
    11101 tcccccgacc ttgctattgg acgtttgagc ggcggcaatc agcaaaaagc gatcctcgcc
    11161 cgctgtctgt tacttaaccc gcgcatcctc attcttgatg aacctaccag gggtattgat
    11221 attggcgcga aatatgagat ctacaaatta attaaccaac tcgtccagca gggtattgcc
    11281 gttattgtca tctcttccga attacctgaa gtgctcggcc ttagcgatcg tgtactggtg
    11341 atgcatgaag ggaaactaaa agccaacctg ataaatcata acctgactca ggagcaggtg
    11401 atggaagccg cattgaggag cgaacatcat gtcgaaaagc aatccgtctg aagtgaaatt
    11461 ggccgtaccg acatccggtg gcttttctgg gctgaaatca ctgaatttgc aggtcttcgt
    11521 gatgattgca gctatcatcg caatcatgct gttctttacc tggaccaccg atggtgccta
    11581 cttaagcgcc cgtaacgtct ccaacctgtt acgccagacc gcgattaccg gcatcctcgc
    11641 ggtaggaatg gtgttcgtca taatttctgc tgaaatcgac ctttccgtcg gctcaatgat
    11701 ggggctatta ggtggcgtcg cggcgatttg tgacgtctgg ttaggctggc ctttgccact
    11761 taccatcatt gtgacgttgg ttctgggact gcttctcggt gcctggaacg gatggtgggt
    11821 cgcgtaccgc aaagtccctt catttattgt caccctcgcg ggcatgttgg catttcgcgg
    11881 catactcatt ggcatcacca acggcacgac tgtatccccc accagcgccg cgatgtcaca
    11941 aattgggcaa agctatctcc ccgccagtac cggcttcatc attggcgcgc ttggcttaat
    12001 ggcttttgtt ggttggcaat ggcgcggaag aatgcgccgt caggctttgg gtttacagtc
    12061 tccggcctct accgcagtag tcggtcgcca agctttaacc gctatcatcg tattaggcgc
    12121 aatctggctg ttgaatgatt accgtggcgt tcccactcct gttctgctgc tgacgttgct
    12181 gttactcggc ggaatgttta tggcaacgcg gacggcattt ggacgacgca tttatgccat
    12241 cggcggcaat ctggaagcag cacgtctctc cgggattaac gttgaacgca ccaaacttgc
    12301 cgtgttcgcg attaacggat taatggtagc catcgccgga ttaatcctta gttctcgact
    12361 tggcgctggt tcaccttctg cgggaaatat cgccgaactg gacgcaattg cagcatgcgt
    12421 gattggcggc accagcctgg ctggcggtgt gggaagcgtt gccggagcag taatgggggc
    12481 atttatcatg gcttcactgg ataacggcat gagtatgatg gatgtaccga ccttctggca
    12541 gtatatcgtt aaaggtgcga ttctgttgct ggcagtatgg atggactccg caaccaaacg
    12601 ccgttcttga ttttgataaa aattttctca aagccggtta cgtattaccg gttttgagtt
    12661 tttgcatgat tcagcaggaa aagaaccatg tttactaaac gtcaccgcat cacattactg
    12721 ttcaatgcca ataaagccta tgaccggcag gtagtagaag gcgtagggga atatttacag
    12781 gcatcacaat cagaatggga tattttcatt gaagaagatt tccgcgcccg cattgataaa
    12841 atcaaggact ggttaggaga tgtcgtcatt gccgacttcg acgacaaaca gatcgagcaa
    12901 gcgctggctg atgtcgacgt ccccattgtt ggggttggcg gctcgtatca ccttgcagaa
    12961 agttacccac ccgttcatta cattgccacc gataactatg cgctggttga aagcgcattt
    13021 ttgcatttaa agagaaaggc gttaaccgct ttgcttttta tggtcttccg gaatcaagcg
    13081 gcaaacgttg ggccactgaa cgcgaatatg catttcgtca gcttgtcgcc gaagaaaagt
    13141 atcgcggagt ggtttatcag gggttagaaa ccgcgccaga gaactggcaa cacgcgcaaa
    13201 atcggctggc agactgacta caaacgctgc caccgcaaac cgggattatt gccgttactg
    13261 acgcccgagc gcggcatatt ctgcaagtat gtgaacatat acatattccc gtaccggaaa
    13321 aattatgcgt gattggcatc gataacgaag aactgacccg ctatctgtcg cgtgtcgccc
    13381 tttcttcggt cgctcagggc gcgcggcaaa tgggctatca ggcggcaaaa ctgttgcatc
    13441 gattattaga taaagaagaa atgccgctac agcgaatttt ggtcccacca gttcgcgtca
    13501 ttgaacggcg ctcaacagat tatcgctcgc tgaccgatcc cgccgttatt caggccatgc
    13561 attacattcg taatcacgcc tgtaaaggga ttaaagtgga tcaggtactg gatgcggtcg
    13621 ggatctcgcg ctccaatctt gagaagcgtt ttaaagaaga ggtgggtgaa accatccatg
    13681 caatgattca tgccgagaag ctggagaaag cgcgcagtct gctgatttca accaccttgt
    13741 cgatcaatga gatatcgcaa atgtgcggtt atccatcgct gcaatatttc tactctgttt
    13801 ttaaaaaagc atatgacaca acgccaaaag agtatcgcga tgtaaatagc gaggtcatgt
    13861 tgtagcgatg atgagaattg tcggcgtcac atcagggaac tcagcacagc cacctgatgc
    13921 gagcaaaagc cttatccgac cgacaaatca gataaggctc gaggaaatga aattacatat
    13981 gcgccgcgat taaccgttgg ttatcctggt acattgcgaa caggtagttg ttataactct
    14041 tccctttggt cgagtagccc ttcagcttgt gaatcatcgc tgtggcagtc acttcctgat
    14101 ccgctttacg cagctgtgca cgcgatttac ggaacgaaga gtaagccggg tgcgtattca
    14161 ggttagtgac ataggcgctc accgattctt tgacagaact aaactgtgag taccctttca
    14221 ctttacccgg cgcattggta caacgtcctt tcatgcattt catgccgaac agattgttat
    14281 tgttgcgcgc cagcttcgac gttccccaac cgctttctgc tgcagccatc gtcgccacca
    14341 tactggtagg gataatgtct acgcgttcaa gcaaggtatt ccacgggatt tttcgcgtat
    14401 taccagacca cttcaccttg tagcgtttgg cgatgtcttt cagacgcgca cgctcagcag
    14461 gtgaccattg gccctgatac tgttttgaaa tcagccagtt acgttctgca gtaatggctg
    14521 cattttggct ggtaatgtaa ggcattacgg tccggagaaa cgcctttttc cttggtgttc
    14581 cggaagggta ttttcgcaaa tcaggaagtg aactactctt tgcactattg cgagaatact
    14641 cttgtttact gcttacctgt ttattactag ctttagttaa gtgggacttt tgactcgctg
    14701 ttgttgtgtg cgtcttcgct aacacctcac tcgaaaacac cagagtgagt aacataagaa
    14761 tcatcgcccc atatcgtcgt atgggagtca aaatcatcag gtctcctggt cggatttaat
    14821 cattccaaca ccttatattt ttcacaaatt tgagagttga atctcaaatc atatcaaaaa
    14881 tagctgtcaa gagcacccca aggaatagtc caaatctgaa actatgtcac gagttaacga
    14941 ttcagattgg cgctaaatcg cagaaaatgt gggggttatc gcaaaattca gccgtttttt
    15001 gcgcgcgatc gctcaccctt gcttctcatc ctgtggactt accgctcagg gatgagtttt
    15061 gtctggctta tcgctggcaa actgtctgaa atcgcagcaa taaggactca tccaccatga
    15121 aactcgccgc ctgttttctg acactccttc ctggcttcgc cgttgccgcc agctggactt
    15181 ctccggggtt ccctgccttt agcgaacagg gaacgggaac atttgtcagc cacgcgcagt
    15241 tgcccaaagg tacgcgtcca ctcacgctaa attttgacca acagtgctgg cagcctgcag
    15301 atgcgataaa actcaatcag atgctttccc tgcaaccttg tagcaacacg ccgcctcaat
    15361 ggcgattgtt cagggacggc aaatatacgc tgcaaataga cacccgctcc ggtacgccaa
    15421 cattgatgat ttccatccag aacgccgccg aaccggtagc aaacctggtc cgtgaatgcc
    15481 cgaaatggga tggattaccg ctcacgctgg atgtcagcgc cactttcccg gaaggagccg
    15541 ccgtacggga ttattacagc caacaaattg cgatagtgaa gaacggtcaa ataacgttac
    15601 aacccgctgc taccagcaac ggtttactcc tgctggaacg ggcagaaact gacacatccg
    15661 cccctttcga ctggcataac gccacggttt actttgtgct gacagatcgt ttcgaaaacg
    15721 gcgatcccag taatgaccag agttacggac gtcataaaga cggtatggcg gaaattggca
    15781 cttttcacgg cggcgattta cgcggcctga ccaacaaact ggattacctc cagcagttgg
    15841 gcgttaatgc tttatggata agcgccccat ttgagcaaat tcacggctgg gtcggcggcg
    15901 gtacaaaagg cgatttcccg cattatgcct accacggtta ttacacacag gactggacga
    15961 atcttgatgc caatatgggc aacgaagccg atctacggac gctggttgat agcgcacatc
    16021 agcgcggtat tcgtattctc tttgatgtcg tgatgaacca caccagctat gccacgctgg
    16081 cggatatgca ggagtatcag tttggcgcgt tatatctttc tggtgacgaa gtgaaaaaaa
    16141 cgctgggtga acgctggagc gactggaaac ctgccgccgg gcaaacctgg catagcttta
    16201 acgattacat taatttcagc gacaaaacag gctgggataa atggtgggga aaaaactgga
    16261 tcagaacgga tatcggcgat tacgacaatc ctggattcga cgatctcact atgtcgctgg
    16321 cctttttgcc ggatatcaaa accgaatcaa ctaccgcttc tggtctgccg gtgttctata
    16381 aaaacaaaac ggatactcac gctaaagtca tcgaaggctt tacgcctcgc gattacttaa
    16441 cccactggtt aagtcagtgg gtccgcgact atgggattga tggttttcgg gtcgataccg
    16501 ccaaacatgt tgagttgccc gcctggcagc aactgaaaac cgaagccagc tccgcgcttc
    16561 gcgaatggaa aaaaactaac cccgacaaag cattagatga caaacctttc tggatgaccg
    16621 gtgaagcctg gggccacggc gtgatgcaaa gtgactacta tcgccacggc ttcgatgcga
    16681 tgatcaattt cgattatcag gagcaggcgg cgaaagcagt cgattgtctg gcgcagatgg
    16741 atacgacctg gcagcaaatg gcggagaaat tgcagggttt caacgtgttg agctacctct
    16801 cgtcgcatga tacccgtctg ttccgtgaag ggggcgacaa agcagcagag ttattactat
    16861 tagcgccagg cgcggtacaa atcttttatg gcgatgaatc ctcgcgtccg ttcggtccta
    16921 caggttctga tccgctgcaa ggtacacgtt cggatatgaa ctggcaggat gttagcggta
    16981 aatctgccgc caacgtcgcg cactggcaga aaatcagcca gttccgcgcc cgccatcccg
    17041 caattggcgc gggcaaacaa acgacacttt cgctgaagca gggctacggc tttgttcgtg
    17101 agcatggcga cgataaagtg ctggtcatct gggctgggca acagtgactt ttccggcttc
    17161 ccgttcgtca gtacctcggg aagccgccaa ccaggataaa atgtcagccc taatcagcgt
    17221 tgcgggataa agcaccgctc actcttcaac agaccgattt gcaccccagc aaatgtagcg
    17281 ttattgttac cttccttgct acagagttcg acagatatcc cgctatgaca ttctcccttt
    17341 ttggtgacaa atttacccgc cactccggca ttacgctgct gatggaagat ctgaacgacg
    17401 gtttacgcac gcctggcgcg attatgctcg gcggcggtaa tccggcgcag atcccggaaa
    17461 tgcaggacta cttccagacg ctactgaccg acatgctgga aagtggcaaa gcgactgatg
    17521 cactgtgtaa ctacgacggt ccacagggga aaacggagct actcacactg cttgccggaa
    17581 tgctgcgcga gaagttgggt tgggatatcg aaccacagaa tattgcacta acaaacggca
    17641 gccagagcgc gtttttctac ttatttaacc tgtttgccgg acgccgtgcc gatggtcggg
    17701 tcaaaaaagt gctgttcccg ctggcaccgg aatacattgg ctacgctgac gccggactgg
    17761 aagaagatct gtttgtctct gcgcgtccga atattgaact gctgccggaa ggccagttta
    17821 aataccacgt cgattttgag catctgcata ttggcgaaga aaccgggatg atttgcgtct
    17881 cccggccgac gaatccaaca ggcaatgtga ttactgacga agagttgctg aagcttgacg
    17941 cgctggcgaa tcaacacggc attccgctgg tgattgataa cgcttatggc gtcccgttcc
    18001 cgggtatcat cttcagtgaa gggcgcccgc tatggaatcc gaatatcgta ctgtgcatga
    18061 gtctttccaa gctggggctg cctggctccc gctgcggcat tatcatcgcc aatgaaaaaa
    18121 tcatcactgc catcaccaat atgaacggca ttatcagcct ggcacctggc ggtattggtc
    18181 cggcgatgat gtgtgaaatg attaagcgta acgatctgct gcgcctgtct gaaacagtca
    18241 tcaaaccgtt ttactaccag cgtgttcagg aaactatcgc catcattcgc cgctatttac
    18301 cggaaaatcg ctgcctgatt cataaaccgg aaggagccat tttcctctgg ctatggttta
    18361 aggatttgcc cattacgacc gagcagctct atcagcgcct gaaagcacgc ggcgtgctga
    18421 tggtgccggg gcacaacttc ttcccgggac tggataaacc gtggccgcat acgcatcaat
    18481 gtatgcgcat gaactacgta ccagagccgg agaaaattga ggcgggggtg aagattctgg
    18541 cggaagagat agaaagagcc tgggctgaaa gtcactaaaa attgcctgat gcgctacgct
    18601 tatcaggcct acatgatttc tgcaatatat tgaatttgga agaatttgta ggccggataa
    18661 ggcgtttacg ccgcatctgg cataaacaaa gcgcactatt ccggcgttga gaaacgccgg
    18721 aaaacgtttc aaacaggctg ctgttgtagc cgatgccgct gcactttctc gacatccatg
    18781 cactgcaacg cctttgtcgg gcaggcttca acacaggccg gccccgtctc ccgatgccag
    18841 cacagatcgc acttaatcgc cctcgccttt ttgcgcgaag aaacgacctc catcgcacca
    18901 aacgggcaag ccagcataca gcttttacag ccaatgcaac gtgtttgttc aacgaaaata
    18961 tgcccatgtt cgcggcttat cgcgtcaaca gggcagacat tcgcgcacgg tgcatcttca
    19021 cactgatgac aggctactgg cgtggtccag ctgtggtctt taatgacacg aatacgggaa
    19081 ataaactcgt ctggtgacaa cgcagcgcaa tcctgattct cctgatgcga cactgcgcaa
    19141 gccacttcac aggtacggca accgatacat ttcgtcgcat ccgcaataat aaaccggttc
    19201 atgatcattc ctttgcacta aaacagcaga gtgccagaga gcgcgtccct actgctttga
    19261 tccaacgaag gaaatgttaa agttttggca cttgtccaaa gcgttatgtg atcgcgctca
    19321 ggtcatccct gacagtaaat cccagttcat tagaaatagc ctgcgcggtt tcacgcagtg
    19381 gtttcaggag atttttctct cccacctgtt tcagacgtga tgtcgaaagc gaaatcgaca
    19441 cggcgtacgg cacccgccca tgaatatcaa acaccggaac agcaatacag gagacgccga
    19501 gttcgttttc ttctctgtcc atcgccgctc cgctttcacg aatgtgcgcc agttcgtcga
    19561 acatcgcggg cagctcggta atggtattgc gggttaacgg ctggatctca tgctgatggt
    19621 tttcccagta tgacttcacg tagtccgggt gaccaaacgc catgtagatc ttgcccattg
    19681 cggaacagta gagcggcata tgctggccaa tataggcacg ggttcgcagc atcccggttg
    19741 tgggttccag cttataaatc aaaatagcgt gatcgtcttc gcggctggag aagttaatgg
    19801 tttcaccagt ggcgatgttc agtgcctcaa gatgcggagc ggcgatatga atgatattca
    19861 gcgaagacag cgccttctgc ccgacggcaa taaatttggt agtcaggcgg taactccctg
    19921 cggcgggcgc ggtggtcaca tagccgcagg actgtaatcc ctgcaataag cgatggacgg
    19981 tactcttatt taaaccagcc agctccgaaa gatgcgccaa cggacaaccg tttggatagt
    20041 tgctcaaaat ctcaatcagc atcaacccgc gaaacagact ctggcttccg gctggacgct
    20101 ctttttcctg cgccatctcg ttctcttttt ttcccatcac ttctttcccc attttgtcgc
    20161 gtcctgatgg tagcgcaaag tgtgccgtag ttcacgatct cgacagataa tttataacca
    20221 cttgattttt atgtcttttg aaattcatca atcagattgc tttgttaaaa agtgatcgat
    20281 atatttgaaa tctagttccg catattgaaa ttttaagcca aaaaagcgat caaaaaaaca
    20341 aggaagccta ggatgaaagt gacatttgag cagttaaaag cagcctttaa tcgggtctta
    20401 atttcacgcg gcgttgacag cgaaacggct gacgcctgtg cagagatgtt cgcccgcacc
    20461 accgaatccg gcgtttattc tcacggcgtt aatcgtttcc ctcgtttcat tcaacaactg
    20521 gaaaacggcg atatcattcc tgatgcccaa cccaaacgta taaccagcct cggcgcaatt
    20581 gaacagtggg atgcccagcg ttcgatcggc aacctgacgg cgaaaaagat gatggatcgc
    20641 gccattgaac tggctgccga tcacggtatt ggtctggtgg cacttcgtaa tgccaaccac
    20701 tggatgcgcg gcggcagcta cggctggcag gcggcggaaa aaggctatat tggcatttgc
    20761 tggaccaact ccatcgccgt aatgccgccg tggggcgcaa aagagtgtcg catcggcact
    20821 aacccgctga tcgtcgccat tccttccacg ccgatcacca tggtcgatat gtcgatgtcg
    20881 atgttctctt acggcatgtt agaagttaac cgcctggcag gccgtcagct cccggtcgat
    20941 ggtggctttg atgatgaggg caatttgacc aaagaacctg gcgttatcga gaagaatcgc
    21001 cgcattttgc cgatgggcta ctggaaaggt tctggcatgt cgattgtgct ggatatgatc
    21061 gctactctcc tttccgacgg cgcatcggtt gccgaagtca cccaggacaa cagcgacgaa
    21121 tacggcattt cacaaatttt tattgccatt gaagtggaca agcttatcga cggtcccacc
    21181 cgcgatgcca agctgcaacg tatcatggat tacgttacta ccgctgaacg cgctgacgaa
    21241 aaccaggcca tccgcttacc cggccacgaa tttactaccc tgctggccga aaaccgccgt
    21301 aacggcatca ctgttgatga cagcgtgtgg gccaaaatcc aggcgttatg aggagataag
    21361 tcatgatttt tggacatatc gcgcagccca atccgtgccg tttgcccgcc gccattgaaa
    21421 aggcgctcga ttttctgcgc gctaccaact tcaacgtcct ggagccgggc gttgtcgaaa
    21481 tcgacggcaa gaatatttat gcgca
//
LOCUS       DAEEUE010000065        21277 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.065, whole genome shotgun sequence.
ACCESSION   DAEEUE010000065 DAEEUE010000000
VERSION     DAEEUE010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21277
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.065"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..446)
                     /locus_tag="KLK84_002631"
     CDS             complement(<1..446)
                     /locus_tag="KLK84_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HBE2009903.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLV"
     gene            complement(501..1352)
                     /gene="manZ"
                     /locus_tag="KLK84_002632"
     CDS             complement(501..1352)
                     /gene="manZ"
                     /locus_tag="KLK84_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HBE2009904.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1365..2165)
                     /gene="manY"
                     /locus_tag="KLK84_002633"
     CDS             complement(1365..2165)
                     /gene="manY"
                     /locus_tag="KLK84_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBE2009905.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2228..3199)
                     /gene="manX"
                     /locus_tag="KLK84_002634"
     CDS             complement(2228..3199)
                     /gene="manX"
                     /locus_tag="KLK84_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBE2009906.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMVPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3662..5212
                     /gene="yoaE"
                     /locus_tag="KLK84_002635"
     CDS             3662..5212
                     /gene="yoaE"
                     /locus_tag="KLK84_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HBE2009907.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5216..6814)
                     /locus_tag="KLK84_002636"
     CDS             complement(5216..6814)
                     /locus_tag="KLK84_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBE2009908.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6945..8309)
                     /gene="sdaA"
                     /locus_tag="KLK84_002637"
     CDS             complement(6945..8309)
                     /gene="sdaA"
                     /locus_tag="KLK84_002637"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBE2009909.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8493..9071)
                     /locus_tag="KLK84_002638"
     CDS             complement(8493..9071)
                     /locus_tag="KLK84_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBE2009910.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            complement(9075..10436)
                     /gene="pabB"
                     /locus_tag="KLK84_002639"
     CDS             complement(9075..10436)
                     /gene="pabB"
                     /locus_tag="KLK84_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HBE2009911.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10510..10689
                     /locus_tag="KLK84_002640"
     CDS             10510..10689
                     /locus_tag="KLK84_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HBE2009912.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10809..11168)
                     /locus_tag="KLK84_002641"
     CDS             complement(10809..11168)
                     /locus_tag="KLK84_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HBE2009913.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11530..11874)
                     /locus_tag="KLK84_002642"
     CDS             complement(11530..11874)
                     /locus_tag="KLK84_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBE2009914.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12006..13916
                     /locus_tag="KLK84_002643"
     CDS             12006..13916
                     /locus_tag="KLK84_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBE2009915.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13974..14669
                     /gene="tsaB"
                     /locus_tag="KLK84_002644"
     CDS             13974..14669
                     /gene="tsaB"
                     /locus_tag="KLK84_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBE2009916.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14709..15290
                     /gene="yeaY"
                     /locus_tag="KLK84_002645"
     CDS             14709..15290
                     /gene="yeaY"
                     /locus_tag="KLK84_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HBE2009917.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15495..17180
                     /gene="fadD"
                     /locus_tag="KLK84_002646"
     CDS             15495..17180
                     /gene="fadD"
                     /locus_tag="KLK84_002646"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HBE2009918.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHALEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17262..18377
                     /gene="rnd"
                     /locus_tag="KLK84_002647"
     CDS             17262..18377
                     /gene="rnd"
                     /locus_tag="KLK84_002647"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HBE2009919.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18431..19396)
                     /gene="yeaX"
                     /locus_tag="KLK84_002648"
     CDS             complement(18431..19396)
                     /gene="yeaX"
                     /locus_tag="KLK84_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HBE2009920.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGIPPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19452..20069)
                     /gene="yeaW"
                     /locus_tag="KLK84_002649"
                     /pseudo
     CDS             complement(19452..20069)
                     /gene="yeaW"
                     /locus_tag="KLK84_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
     gene            complement(20411..20977)
                     /locus_tag="KLK84_002650"
     CDS             complement(20411..20977)
                     /locus_tag="KLK84_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE2009921.1"
                     /translation="MPPEIMRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTY
                     PSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELL
                     EDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRK
                     KILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            21189..>21277
                     /locus_tag="KLK84_002651"
     CDS             21189..>21277
                     /locus_tag="KLK84_002651"
                     /inference="COORDINATES: protein motif:HMM:NF025073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE2009922.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT         5269 a         5272 c         5467 g         5269 t
ORIGIN      
        1 acgagaagct tataaatttt ttccagatcg tcgatatttc gaacgcggat taacagccga
       61 cgctgttcta attgcatcac caacacgcca tcttccgaca agttcatcgc tttgattcgg
      121 ctatattcaa tccagacatt ggcgaagaaa aaaccttttt gtttaaagat gatcttcgga
      181 atgcggatcc agaatatata aaaacccatc agagccagtg cacttaataa ccatgttgtt
      241 attagcgcgc catgattcgt gacgttgtta tagataagaa tgacaatcaa tccgacgaag
      301 ataacgctat cgatgcgacc acgccggagc aaaggaattg ccagcagggt ggggccgtta
      361 cggcggggca tgatgaactg atcgtagatc gcgaaggcca ggagtgcggc gatgaaaaga
      421 atcagtacca ggtccgtgat tgtcattaat cctccagata aaaaaacggg gccaaaaggc
      481 cccggtagtg tacaacagtc ttacagtccc agcaggccgc aagcgtaacc agcgataccg
      541 atgacgaaga agccaacgat gatccacagc gggttaactt ttttgcgcag cagccacata
      601 caagcaaatg tcagcagcag tggtacaagg cctggcatta actggtcaag aatagtttgc
      661 acggtggtga cacgggtctg tccatcctgc ccggtgatgg tcgaaaccac cagcgggatg
      721 ttgacatgtg tccacttgtt aaccaatgcc cccatgacaa acaggccgag gatagacgcc
      781 ccttccgtca gtttttgcag gaagccacca cccatatctt taacgatatc gatacctttg
      841 gagtaaccat acgctacgcc gtagtaacgg gttgccagac gcaccaggtt aaacaggatg
      901 aagaacagca gcggacctaa caggctgccg ctcatcgcga taccggcacc cagtgctgca
      961 aataccggac gtacggttcc ccagaagatc gggtcgccta caccagccag cggccccatc
     1021 aaaccgactt tgataccgtt gatagcaccg tcatcgatct ctgcgccatt agcacgctgt
     1081 tcttccagcg ccagggttac gccgagaatc ggtgcagcca cgaacggctg ggtgttaaag
     1141 aactccaggt gacggcgaat agcttgttta cgagcttcgt tgttctcagg gtagaggcga
     1201 cgaattgccg gtaccataga gaagcagaaa cccagtgcct gcatacgttc gaagttccat
     1261 gaaccctgga agaggttaga acgcaggaat acgccacgaa tatcactttg agtgagtttt
     1321 ttctcggtgg tagtttgagt tgtatcaacc atttcgctca cctgttagtc cagttcgtta
     1381 tcgagatcgt tgttaccagc tgcctgagca ggcgcaccgg ctacgcggtt gtatttcggg
     1441 ctaagttgga tgtagagcac tgccataaca gtaccaatca cacccagagc aaccaggtta
     1501 aagttggtga atgctgcggt tacgaagccg aggtagaaga acggcatcag gtagccagca
     1561 cgcatcatgt tgataaccat cgcataacca accaccacga tcatgccccc ggcgatattc
     1621 agaccattgg tcaccacttc cggaatcgca ttcagcatgt tctgtacttc gctggtacca
     1681 acagacagcg caacgatgac ggccggaata gccacacgca ttgcttgcag gaacagagaa
     1741 gaaacgtgga tccaggaaat cgctgtcagg ttgccgttat cagcagcctt atccgcagcg
     1801 tgctggaaag caacggtaat agtacgaacg atgatggtca gtacctggcc cgcagcggcc
     1861 agagggattg ccagtgcgat acctgcacca atgctctgat gacctgcgat aaccagaatg
     1921 gtagaaatga tagaagccag agcggcgtca ggcgcaactg cagcaccgat gttcatccag
     1981 cccagcgcga tcatttccag cgtaccaccg ataataatac cggttttcat atccccaaga
     2041 acgataccca ccagggtaca cgcgattagc ggacggtgaa actgaaattc atcgaggatt
     2101 gatcccatac ctgcgataca ggctacgata aataccagca caatttgaag agtggtaatc
     2161 tccattgtac ttctcctgtt tacgtaagac ttaagtgtga aaactgaatg ataatcaaca
     2221 caatacgtta cttatcgatt ttgctgatca gatccatcat tttcagtttc ggatcggtgg
     2281 aaaccttacg gacttccagc tcaataccgc gtgcattcag ttttttgaac gcctcgatat
     2341 ctttttcatc aaccgaaacc gcgttattca cctgggtttt accctgacgg aatgccatac
     2401 caccgacgtt aacagaggtg attttcacgc cgccttcaac gagacgctct acatctgttg
     2461 ggttggtaaa taacagcatt acgcgttcgc cagcatattt cgggttgttg tagacgcgaa
     2521 tcattttggc aacatcaact acgtgtgctg ttacgcccgg aggtgcaacc tgggtgagca
     2581 gtgtcttacg aacggtatcc gcagccactt catcactaac aacaataata cgggagacat
     2641 tggtttcttt ggtccagcgg gtggcgacct gaccgtgaat cagacggtcg tcgatacgcg
     2701 caaggccaat aaccatgtag tcgtttggca ccattggttt tgccggagtt ggagccgctt
     2761 ttggtgctgc ggcagcgggt gctggcgcgg ctttttcaac cggtttggct ttcagtgctt
     2821 tcacgccttc acggcctgtt tctactgcca gcgcaaccag ttcatcaaag ctcgggtcat
     2881 catcacgggc cattaacgtt tccacgagca ttggaatgtt aacgcctgca atgacttcat
     2941 aatgctcttt gtcgacgaca atgcggctgg cagcattgaa cgggctgcct ccccatgtat
     3001 caacgagaaa cagcacgcct ttagtggtgt cgagttttgc caactgagcg ttgtattttt
     3061 caatcagcgt ttcggcattt tcacctggaa cgaaatcgat ccagccgacg ttttcctgct
     3121 cgcctaacag catttctgcc gttttaagca actgctctgc agcccaacca tgtgtgccta
     3181 taacaatagc aatggtcact tgctacctcc tttattatcg ttaacacctc aacgtgccag
     3241 atgtattttt gaatcgtcgt ccacaatcga atcgattcag ataagggcta caaccaaaaa
     3301 cccctgcgtt tcgcgattta ttttagatat cgaaaaaatt attttatgtg atgaagatcc
     3361 gtaatttaac tttcgattat cagaaatttc gaaaggtaaa atatccttgt cacattcgtt
     3421 tgcaaaggaa ggtaaatctt tgccaaatca gaggcgtctc tgatatgttt aactcccgtt
     3481 taacaaccat ggagtatagg gcagtagccc gcagtatgga tcgtcaccga cgtcatttca
     3541 gcatcaggcc ttttaacgcc tgcctttctg gcactctatg ccgcaccttt cgtttgcatt
     3601 ttgtcgttac gcctgcatta tttctggcgt cgaatagcta ttccttaagc aggagcttgt
     3661 catggaattc ttaatggacc cctcaatttg ggcggggcta ctcacgcttg ttgttctcga
     3721 aattgtgctg ggtatcgata acctggtctt catcgccatt cttgctgaca aactgccgcc
     3781 aaaacaacgc gataaagcgc gtttgctggg gttatcactg gcgctgatta tgcgtctggg
     3841 gctgctgtcg ctgatttcat ggatggtcac gctgaccaaa ccgctattta ccgtcatgga
     3901 tttctccttc tccggacgcg atctgattat gttgttcggg gggatattct tgctgttcaa
     3961 agcaacaacc gaactgcatg aacggctgga aaaccgcgat catgattccg gccacggtaa
     4021 aggctacgcc agtttctggg tggtcgtcac acagattgtc atccttgacg ccgtcttctc
     4081 gttggatgcg gtaattactg cagtagggat ggttaaccat ctgccggtga tgatggcggc
     4141 ggtagtgatt gcgatggcgg ttatgttgct ggcatcgaaa ccgctgacgc gattcgttaa
     4201 ccagcacccc acggtggtgg tgctctgtct gagcttcctg ttaatgattg gtctgagtct
     4261 ggtggcagaa ggtttcggtt tccacattcc gaaaggttac ctgtatgccg cgattggctt
     4321 ctcgatcatt attgaagtgt ttaaccagat tgcgcgtcgc aactttattc gtcaccagtc
     4381 gactttgccg ctgcgagcgc gtactgccga tgccatcctg cgtttgatgg gcgggaaacg
     4441 tcaggccaat gttcagcacg atgccgataa cccgatgccg atcccggaag gtgcatttgc
     4501 cgaagaagaa cggtacatga ttaacggcgt actgacgctg gcgtcgcgtt ctctgcgcgg
     4561 gatcatgacg ccgcgcggtg aaataagctg ggttgacgct aatctcgggg tcgatgaaat
     4621 ccgcgagcaa ctgctctctt caccgcacag tctgttcccg gtatgtcgcg gtgaactgga
     4681 tgaaatcatc ggtatcgtac gtgctaaaga actgctggtg gcgctggaag agggcgttga
     4741 tgtggcggcg attgcttcgg cgtctccggc gattatcgtc ccggaaaccc tcgatccgat
     4801 caacctgctg ggcgtgctgc gtcgcgctcg cgggagcttt gttatcgtga ccaacgagtt
     4861 tggtgtggta caaggcttgg tcacgccgct ggatgtgctg gaagccattg cgggtgaatt
     4921 cccggacgct gacgaaacgc cggaaatcat taccgatggt aacggctggc tggtaaaagg
     4981 cggtacagat ttgcatgcct tgcagcaggc gcttgatgtt gagcaccttg ccgatgacga
     5041 tgatatcgcg acggtcgcgg gcctcgtgat ctcggcaaat ggtcacattc cccgtgtggg
     5101 cgatgtgatt gatgtagggc cactgcatat caccatcctt gaagccaatg attatcgtgt
     5161 tgatctggtt cgcattgtta aagagcaacc ggcgcacgat gaagatgagt aagcgttaac
     5221 gtaacgacat aatgggcgtg atatgtccat tatgccgggc gggcggcggt tggctgcccg
     5281 ccagccattt gggaaaatca cgtagcggca tcggctgtgc gtacaaatac ccttgcaaaa
     5341 tatgcacgcc atgacggcgc aaatacttcg cctgttcttg cgtttccacg ccctccgcca
     5401 ccagttcaat attcagtctt tgccccagcg cgatgatgat atcggtcacc gtcgagttaa
     5461 ccgcgtcaga acctatagct gcggtaaatg acttatcaat tttcagcacg tcaggacgta
     5521 atgtttcaag ccaggaaaac gagctgttgc cggtgccgaa gtcatcaatc gccagtttga
     5581 cgtttttacg atgcagctcg cgggcaatcc gataatcaac atccagtaag gcatcgcgtt
     5641 cggtgatttc gaggatcagt tgctgaatcg ggtgagcgct aaaccagtac tgattgagat
     5701 cttttatcag cacaccacgg cgaaaatggc tgggtgcgac gttaatgcca acatgaaact
     5761 gactactcat cgggaaaaca tggtgctgac gaatggtttc tgccatcaca tagtgggtca
     5821 gtggcacaat taaatgatgt tcttccgcga taggaataaa cacatccggt gaaatccagc
     5881 cctgacgcgg attattccag cgcagcaaaa tctctacacc aatacattgc tggctgcgcg
     5941 cattaagcaa aggctgacag aacaattcga actcatgttg cgccaggccc agattaattt
     6001 cgcgggaaaa gctcatccgg taagcggtcg ccagccaggc gatatagccc accagtaaac
     6061 ttagcagcac cgccagcggt aattgtgttg gaagatagtg ccatgccagc gccgtagccc
     6121 ccggaccatt tacgttaatg gtaaaaggaa agctttgcga agaaacctga tagttttcat
     6181 tgtcttcagg ttgcggaagg gaatctacca gcccattacc atagagcaaa tgccgtttgt
     6241 ccaccgtcag gctggcgcta ctgatttgcg gcagttgtgg ctcaaggagc attgccgtca
     6301 gtaagtcgat gttaatcatc tctatgaccc cagcattgct actgcccgct gcaggcgtcc
     6361 attgaatcaa aaccggactg cctttaatca gggcacggtc gatcgtcagg cgtaaaagtg
     6421 gttgcggtgc aggaagttca gccagaatgt ccacgacggg cacattgcga taaccaaaaa
     6481 tgctggagca ataaagtgtg ccgtcttgca ccaggccaat ggatcgcaca gtttggagtt
     6541 ttgccgcctg tttacgtaat ggcaaatggg cgacagagca gggcagacca atcagcggaa
     6601 gcaagacttt gctacctgcc tgtaggggaa gcagtacttt atctaattcc tctacagcgt
     6661 gattggcgaa ttgtactacc cgttgttgat ttaagttacg ctgtgaaata aatcgcacgc
     6721 tcagggtcga agcgagcgta acgagtgcgc aaatcgtaca aacaatcatt cgattacggc
     6781 gataggtttt aatgatccgt tgtgcttttt gcatgcgggc caccctgtaa gcgtatggcg
     6841 acaaacgcct gacgcaacag tggaagtgta gtggggaata ttacagcaga cgagaaagcg
     6901 ggtataaatt cgcccatccg ttgcagatgg gcgagtaaga agtattagtc acactggact
     6961 ttgattgcca gaccaccgcg tgaggtttcg cggtatttgg cgttcatgtc cttaccggtt
     7021 tcgtacatcg tttcgatgac cttatccagc gagacgcgcg gtgcactggt gcggcgcaga
     7081 gccatccgtg cggcgttaat cgccttcaca gaggcaatgg cattacgctc aatgcacggc
     7141 acctgaacct gccctgcaac cgggtcgcag gttaaaccaa ggttgtgttc catgccaatt
     7201 tccgccgcca cgcaaacctg ttccgggcta ccgcccagca gttctgcaag acccgcagca
     7261 gccattgaac aggcaacacc cacttcgccc tggcaaccaa cttccgcacc ggaaatagag
     7321 gcgttcattt tatacaatgc accaatcgcg cccgctgcca taaagtaacg ggtatagatg
     7381 tctgggctga ccgattcaat aaagtggtca tagtaagcca gcactgccgg aacgataccg
     7441 caggcaccgt tggttggcgc agttaccaca cgaccaccgg cggcgttttc ttcgttaact
     7501 gccagcgcaa acatgtttac ccagtcaatg acattcatcg gatcgttaga cagtttatcg
     7561 ctggaaacta acatccggcg cagggcagac gcacgacgtg gcacgcgcag cgggcctggc
     7621 agtacacctt cggtgttcat cccgcgatcg atacatgcct gcatggtttg ccagacatgc
     7681 gcgaaatact cgtcgatctc tttcttgctg tgcagcgcca gttcgttctg catagcgaga
     7741 ccagacagcg aatagccggt ttcattacag tacgcgagca gttcggtggc agatttgaac
     7801 ggatacggca cgctcacttc gttggcagca tcctgaccaa agtgttcttc atcgacgata
     7861 aaaccgccgc cgatggaata ataagttttg ctgtagacga cttcatcgcc gttataggcg
     7921 tggatttgca taccgttttc atgcagcggc aggttgccgt tatgaaaacg catcccgttg
     7981 tcgcgcggga aatccacttc atgccgtccc tgtgccagca gcagacgttc gcgctctttt
     8041 acgtcgcgaa taaaaccggg aatactgtcg atatccacgg tggcaggttc gttacctgca
     8101 agacccataa taatggcgat atcggtgtgg tggcctttac ccgtcagcga cagtgaacca
     8161 taaacgtcca cggcaacgcg agtaacgcta tccagtaagc ctttttcgac cagatcatcg
     8221 acgaactgtt tacccgcctt cataggccct acggtatggg aagatgaggg accaatcccc
     8281 accttaaaca tgtcgaatag actaatcacg ataatactcc tgacaaggtg actggacttc
     8341 cagtaacgaa ctaataacag cgcatagtgt aagagggaac gcggcgactg gcttaactat
     8401 tcacatgaat taaactaatc aaaaaccgcg atttaactaa tattgcgatg attgtctcaa
     8461 gtagtgtttt gtagatgtaa agataagtat agtcaggggt tcacaccaat ttgcagcgcc
     8521 agctcacgaa ttatgcctgc ggtcattccc catacaaaat actgttcgta ccaggacagc
     8581 catacccgat gtgaatcgcc acggcggtag atatctaaag ggtgataacg acccagatgt
     8641 aatgcctggg cgagcggcat ttcaaacacc gccgagactt catcttcact ggcgcgatac
     8701 ggcagatcgg gcgggataat gccgaccact ggggttactt gatagccagt tacgctatcg
     8761 acgggcggca gcacgccgat aacttcaacg gcggaaggcg gtatagcgac ctcttcttca
     8821 gcttcgcgca gcgcggcggc gataactgat gcgtccgtgt catcgactgc acctccaggg
     8881 aatgccactt gtccagcgtg tttacgcaga tgaattgaac gctgagtcag caacaacccc
     8941 ggttgcggtc gacggacgat ggggattaac acggcagcct gacgatgatt taggggttcc
     9001 cggttaattt gcgggcgcaa aagttgaaag cgagataaaa aatcatcaag cgtcaggcta
     9061 cggtattcca cgtcttactt ctccagttgg cgtaatatct tattaacttt atcaaaagtt
     9121 tcctgatatt ccgcttcttc ctggctatcg gcgacaattc caccgcccgc agagcagtat
     9181 atttgtccgt taatggcagt cagcgtgcgg atagtaatgc tggtatccat gttgccgcaa
     9241 aagctcaaat agccaatgct gccgcaccag gcattacgtc gctgcggttc cagttcgtcg
     9301 ataatttcca tagcccgtac tttcggagcc ccggttattg agccaccagg aaaagctgcg
     9361 cgcagcagat cgctggcgtg taactgttct ggtagtcgcg ccgttatagt gctgaccaga
     9421 tgatgcacgg cagggaaggg ttccaccacg aagagctctg gtacttttac cgaaccggct
     9481 acggcaacac gaccgatatc attacgcatt aaatcgacaa tcatcagatt ttcggcacga
     9541 tctttcgctg agttcgccag tttttctgct tgtttgctat cttcctgagg atcgggcagg
     9601 cgtggtagcg tgcctttaat cgggcgggtc tggatttcac tattatcaca aagaataaac
     9661 cgctctggcg aaaggcttaa aattgcaccc tgttcaagac gtaaaaaagc gctaaatggc
     9721 gcgcggttgg cctgattaag ctgaaggaat gcctgccatt catcgccaga ataggtcgca
     9781 tgaaaacgct gggcgagatt cacctgatag caatcaccgc tgtgcagata ttcctgtacc
     9841 tggcgaaatt tttcgccgta ctgctcgtgg gtcatattgg attgccagtc ggaagtgagc
     9901 gtgaaatctt cctgcggcga gaattgctgg ctttccagcc aggcccgacg ggcattgaca
     9961 tcattatgac tcagcaaaga aactgtatga cgctggtggt cgacaatgag cgcccaatcg
    10021 tagataccca ctgccatatc cggcagaacg atatcttgtt ccgcaatttc tggcagtgac
    10081 tcaaaacggc ggcccagatc gtagccaaac aaccccagtg cgccgccctg aaatggcaaa
    10141 tcttcgttat gcgttgggcg aatgtctgcg cgatccagca cctgctggag cacctgtagc
    10201 gggtcatcag tggtcgttgt gcgtttttcg ctttcactaa caacggtttc tttacctaaa
    10261 gtggttaaag tgcaaatcgg atcggcgacc acaatatcaa agcggctata cggatgatcg
    10321 gcatagccgg agtgtaaaag catcgcccac ggcaggtggc ttaagcggga gaaataaaat
    10381 tcagcggcgt cctgacgcca gggtaaagta atcacagtgg gagataacgt cttcatcagt
    10441 cctgactcta ctggctatgt gccgggtagc atgaaataat tagcgccaac aatttagcag
    10501 gagttaacaa tgtttgcagg tttaccttca ctcatccatg aacagcagca aaaagctgtc
    10561 gagcggatcc aggaactgat ggcacaaggg atgagtagcg gccaggcaat cgcgctggtg
    10621 gcagaagagc tgcgtgccaa ccactccggc gagctaatcg tggcgcgttt tgaggatgaa
    10681 gacgagtaac acactaatcc gtatcgattt cgttatagaa gccgatgatc atttgattgt
    10741 gagaaaaagg acgcttttta tatgtgatta aaagcgtcct gacccattta tgctgatata
    10801 aatttaactc agaccagtgc ttttagttct tcctgcatat atgttgaaaa gtattcagga
    10861 tttttaatca cagagcgttc acctgtctcc attttttttg cccatccaac cattttttcc
    10921 gtgaacccag ggttccagcc ttcctggtca gctcgcgccg taatatcagc ggcactcgcg
    10981 ggtactccca gttcttttaa atatttaaag attcccttcg ccgtgctttc attcatcgaa
    11041 cttggtgttg gcgctgatac atccatggca ccaatgaaat ccagggcttt atctattaca
    11101 gcaggcatat tcttatcctt aaaattaacc atgttatcga taaggctata tatgacctta
    11161 atttgcatct aatatcaaag ggattataac gatatggcac gagttaacct gttgacattc
    11221 aggtgtttta aaggtagcag ctcatggtgt gttatccagt aagatggaga aaattaacgg
    11281 tcagcccgca cgctataacc agattgttca gctaagaaag aaaaccagag ttgctaatca
    11341 aagggcgaac acaacggcat ggttatgttt atttatatag ttagttttat agataaaatt
    11401 gaaattataa atataaataa tgaattagca gaagcatcaa cagtaatatc ttattctctg
    11461 gtcgatttat gtaaaaccga ccagtattat cgataaatta tcgtctgact tattttgctt
    11521 cgataaagct tacaccgcag ccacaatctt aatttcaact ttatacttcg ggttcatcaa
    11581 acccgcttgt accgtgcagc gcaccggcgc atgacccgcg acaacccagg catcccacgc
    11641 tttattcatc gccgcgaagt cgtttttatc ggccaggaaa atggtggcat ccagaatgct
    11701 cgatttattg ctgccctgtt tttccagcac ggcgtcaatc tgtgccagcg tgttggcggt
    11761 ttgctcaaag gcatcggcgt cgaggttttc cggtacacca gtgtagtaga gcgtgttgtt
    11821 gtggattact acatcagacc agcgggcttc agcatcgata cgaacgatag tcatcatttt
    11881 gtcctcatta tacttccatt ttcgtgctgc aaagactgcc acaatgtgag gttgccgtca
    11941 ccacttcaac tggcgaaagc gccctcatcc tgacataatc cctgttcaaa tcaacagggg
    12001 gtagtgtgac ggacgatttt gcaccagacg gtcagctggc gaaagcgata ccaggcttta
    12061 agccgcgaga accacagcga cagatggcgg tagccgtcac ccaggcgata gaaaaaggcc
    12121 agccgctggt ggtggaagca ggaaccggta cgggcaaaac ctacgcttac ctggctcctg
    12181 cgctgcgggc gaaaaagaaa gtcattatct cgaccggctc aaaagcgttg caggatcagc
    12241 tctacagccg cgatttgcca acagtctcaa aggcattgaa atatacgggc aacgtggcgc
    12301 tgctgaaagg gcgctcaaac tacctctgcc tcgaacgtct cgaacagcag gcgctggcgg
    12361 ggggcgatct gccggtacaa atcttaagcg atgtgatcct gctgcggtcc tggtctaatc
    12421 aaacagtcga tggtgatatc agcacctgcg tcagcgtggc ggaagattca caggcgtggc
    12481 cgctggtcac cagcaccaac gacaactgtc ttggcagcga ctgcccgatg tataaagatt
    12541 gctttgtggt caaagcacgt aaaaaagcga tggacgccga tgtggtggtg gtaaaccatc
    12601 atctctttct ggcggatatg gtggttaaag agagtggatt tggcgagttg atccctgaag
    12661 cggacgtcat gatcttcgac gaagcccacc agctaccgga cattgccagc cagtattttg
    12721 gtcagtcact ctccagtcga caactgctcg acctggcaaa agacatcacc atcgcctacc
    12781 gcaccgaatt aaaagacacc cagcagttac aaaagtgcgc tgatcgtctt gcccagagtg
    12841 cgcaggattt tcgtctgcaa ctcggtgagc caggttatcg cggtaacctg cgtgagctgt
    12901 tagctaatcc gcaaattcag cgggcatttt tactgctcga tgacaccttg gaactttgtt
    12961 atgatgtggc gaaactgtca ctggggcgtt ccgccttgct ggatgcggca tttgagcgcg
    13021 ccacgttgta tcgcacacgg ctgaagcggc taaaagagat caatcagccg ggctacagct
    13081 actggtacga atgcacttcg cgccatttta ctctggctct cacgccgctc agcgtggcgg
    13141 ataaattcaa agagttaatg gcgcaaaagc ccggtagctg gatcttcacc tcagcaacgc
    13201 tgtcggtgaa cgacgatctg catcatttca cctcgcggct tgggatagaa caggcggagt
    13261 cgttgctatt gccaagcccg tttgattaca gccgccaggc gttactctgt gtgccgcgca
    13321 atctgccgca aaccaaccag ccaggttctg ctcgccagtt agcggcaatg ctgcgaccga
    13381 tcatcgaagc taacaacggt cgttgtttta tgctttgtac ctcgcacgcc atgatgcgcg
    13441 atctggccga gcagttccgc gctaccatga cgcttcccgt tttgttgcag ggggaaacca
    13501 gtaaaggcca actgttgcag cagtttgtca gtgccggtaa tgcgcttctt gtggcaacca
    13561 gtagtttctg ggaaggggtg gacgtgcgtg gcgatacatt gtcattggta attatcgaca
    13621 aattgccgtt tacctcgccg gatgatccac tgttaaaagc gcgcatggaa gattgtcgtt
    13681 tgcgcggtgg cgacccgttc gatgaagtgc aactaccaga tgccgtcatt actctcaaac
    13741 agggggtagg tcgactgatt cgcgacgccg acgatcgtgg cgtgctggtg atttgtgact
    13801 atcggctggt gatgcgtcct tacggcgcga cgtttctcgc cagtctgccg cccgcgccac
    13861 gcacccgtga cattgcccgt gcggttcgtt tccttgcgat accatcctcc aggtaatttg
    13921 ttacggagta tggtatgatg cgcgcctttt tattgatcta agcacgaaga cccatgcgaa
    13981 ttctggctat cgataccgcg acagaggcct gctctgtcgc cctgtggaac gacggtactg
    14041 tcaacgctca ttttgagctt tgccctcgtg aacatactca acgaatctta ccgatggtgc
    14101 aggatatcct gaccaccagc ggaacttccc tgactgatat taacgctctg gcttacgggc
    14161 gcggccccgg tagctttacc ggcgtgcgca ttggtattgg catcgcgcaa gggctggcgc
    14221 ttggcgcgga attaccgatg attggcgtct ccacgctaat gacgatggcg caaggggcgt
    14281 ggcgcaaaaa cggcgcaaca cgcgtgctgt cagccattga cgcgcgaatg ggcgaagttt
    14341 actgggccga atatcagcgt gatgaaaacg gtatctggca cggtgaagaa accgaagccg
    14401 tactcaaacc cgaactcgtt catgaacgaa tgcaacagct ttccggcgaa tgggtgacgg
    14461 tagggacggg ctggcaagcc tggccggatc tcggtaaaga gagcgggctg gttttgcgcg
    14521 atggcgaagt gttactcccc gctgcagaag atatgctgcc gattgcttgt cagatgtttg
    14581 ccgagggtaa aacggtggcg gtggaacatg ccgagccggt ttatttgcgt aacaacgtcg
    14641 catggaagaa acttccgggc aaagaatgaa tcttaagagt atgccctgag aaaaaaagga
    14701 gtcgcatcat ggcggttcaa aagaatgtta tcaaaggcat actggcaggt acgtttgcgc
    14761 taatgctgag cggttgtgtc actgtgccgg acgccattaa aggcagcagt cccacgccgc
    14821 aacaagattt agttcgggtg atgagtgcgc cgcagctgta cgttggtcag gaggcacgct
    14881 ttggtggcaa agtggttgcg gtacaaaacc agcaagggaa aacccgcctg gaaattgcta
    14941 ccgtaccgct ggacagcgga gctaggccga cgctgggaga accttctcgc ggtcgcattt
    15001 atgccgatgt gaacggtttt ctggacccgg tggatttccg tggacaactg gttacggtag
    15061 tcgggccaat caccggtgcg gttgacggca aaatcggcaa tacgccctat aaatttatgg
    15121 tgatgcaagt aacgggttac aaacgttggc atttaaccca gcaggtgatt atgccgcctc
    15181 agccgattga tccatggttt tatggcggtc gtggctggcc ctatggctac ggcggatggg
    15241 gctggtataa tcccggcccc gcgagagtac aaacagttgt aaccgaataa ttgcttgttt
    15301 ttaaagaaaa agaaacagcg gctggtccgc tgtttctgca ttcttacggt aaagataaaa
    15361 ataaatagtg acgcgcttcg caaccttttc gttgggtaat tatcaagctg gtatgatgag
    15421 ttaatattat gttaacagtg tgtatatcat ttggggttgc gatgacgacg aacacgcatt
    15481 ttagaggtga agaattgaag aaggtttggc ttaaccgtta tcccgcggac gttccgacgg
    15541 agatcaaccc tgaccgttat caatctctgg tagatatgtt tgagcagtcg gtcgcgcgct
    15601 acgccgatca gcctgcgttt gtgaatatgg gggaggtgat gaccttccgc aagctggaag
    15661 aacgcagtcg cgcgtttgcc gcttatttgc aacaagggtt ggggctgaag aaaggcgatc
    15721 gcgttgcgtt gatgatgcct aacttattgc aatatccggt ggcgctattt ggcattttgc
    15781 gtgccgggat aatcgtcgta aacgttaacc cgttgtatac cccgcgtgag cttgagcatc
    15841 agcttaacga tagcggcgca tcggcgattg ttatcgtgtc taactttgcc cacgctctgg
    15901 aaaaagtggt tgataaaacc gccgttcagc acgtaattct gacccgtatg ggcgatcagc
    15961 tatctacggc aaaaggcacg gtagtcaatt tcgttgttaa atacatcaag cgcttggtgc
    16021 cgaaatacca tctgccagat gccatttcat ttcgtagcgc actgcataac ggctaccgga
    16081 tgcagtacgt caaacccgaa ctggtgccgg aagatttagc cttcctgcaa tacaccggcg
    16141 gcaccacagg tgtggcgaaa ggcgcgatgc tgactcaccg caatatgctg gcgaacctgg
    16201 aacaggtcaa cgccacctat gggccgttgc tgcatccagg taaagaactg gtagtaacgg
    16261 cactgccgct gtatcacatt tttgccctga ccattaactg cctgctgttt atcgaactgg
    16321 gtgggcagaa cctgcttatc actaacccgc gtgatattcc agggttggta aaagagttag
    16381 cgaaatatcc gtttaccgct atcactggcg ttaacacctt gttcaatgcg ttgctgaaca
    16441 ataaagagtt ccagcagctg gatttctcca gtctgcatct ttccgcaggc ggtgggatgc
    16501 ctgttcagca agtggtggca gaacgctggg tgaaactgac cggacagtat ctgctggaag
    16561 gttatggcct gaccgagtgc gcgccgctgg tgagcgttaa cccgtatgat attgattatc
    16621 atagcggtag catcggtttg ccggtgccat cgacggaagc caaactggtg gatgacgacg
    16681 acaacgaagt accaccgggt caaccgggtg agctttgtgt caaaggaccg caggtgatgc
    16741 tgggttactg gcagcgtccg gatgctacag atgagatcat caaaaatggc tggttacaca
    16801 ccggcgacat cgcggtgatg gatgaagaag gattcctgcg cattgtcgat cgtaaaaaag
    16861 acatgatttt ggtttccggt tttaacgtct atcccaacga gattgaagat gtcgtcatgc
    16921 agcatcctgg cgtacaggaa gtcgcggctg ttggcgtacc ttccggctcc agtggtgaag
    16981 cggtgaaaat cttcgtagtg aaaaaagatc catcgcttac cgaagagtca ctggtgactt
    17041 tttgccgccg tcagctcacg ggatacaaag taccgaagct ggtggagttt cgtgatgagt
    17101 taccgaaatc taacgtcgga aaaattttgc gacgagaatt acgtgacgaa gcgcgcggca
    17161 aagtggacaa taaagcctga gcgttaagtc agtcgtcaga cgccggttaa tccggcgttt
    17221 tttttgacgc ccactaaaga gaaaacaatt tgaattacca aatgattacc acggacgatg
    17281 cgctggcttc tttgtgtgaa gccgtccgtg tctttccggc gatagccctg gatactgaat
    17341 ttgttcgtac gcgcacttat tacccgcagc tggggttgat tcaacttttc gatggcgagc
    17401 atctggcgct aattgatcct ctcgggatca ccgactggtc accgctgaaa gcgatcctgc
    17461 gcgatccgtc catcacaaaa tttctccatg caggcagtga agatctggaa gtgttcctca
    17521 atgtctttgg cgaattacca caacccttga ttgacacgca aatccttgct gccttctgcg
    17581 gacgcccgat gtcatggggt ttcgcttcca tggtggaaga gtattccggc gttacgctgg
    17641 acaagagtga atcgcgcacc gactggctgg ccagaccgct gaccgaacgt cagtgtgaat
    17701 acgcagcggc ggatgtctgg tatctgttac cgatcaccgc caagcttatg gtagaaacgg
    17761 aggcctccgg ctggctacct gcggcgctgg atgaatgccg cctgatgcaa atgcgtcgtc
    17821 aggaagtcgt tgcgccggaa gatgcctggc gtgatatcac caatgcctgg cagttacgca
    17881 cacgccaact ggcctgtctg caactgttag ccgactggcg actgcgcaag gcgcgagagc
    17941 gtgatctggc ggtgaacttt gtcgtgcgtg aagagcattt gtggtcggtc gcgcgttata
    18001 tgccgggaag tttaggcgaa ctggacagcc tgggtttatc cggtagcgaa atccgctttc
    18061 acggtaaaac gctgctggcg ctggtggaaa aagcgcagac attgccggaa gatgccttac
    18121 cgcagccgat gcttaacctg atggacatgc ctggttatcg taaagcgttt aaagcgatta
    18181 agtcgctgat tactgacgtg agcgaaacgc ataagatcag cgccgaattg ctggcatcgc
    18241 gtcggcaaat caaccaactg ctgaactggc actggaaact gaaaccgcag aacaatttgc
    18301 cggagctgat ttccggctgg cgtggtgagc tgatggcgga agcattacac aatttattgc
    18361 aggaatatcc gcagtaaaat cttccgaagc cggactgggc gcgctcagcg ccacatccgg
    18421 ctttggcaaa ctacaaatcc aacaccaggc gtttaccctt cgcacgcgaa caacagatca
    18481 acatactttg ctggctggca cgctcttcat cgctaaaata ctgatcccga tggtcagctt
    18541 caccttccag tattgccgtt tcgcaggttc cgcatacccc ttcacgacat aaacattcca
    18601 ctttcgcggc tttattattt tcaataacct gcaaaatagt catctcttcc ggcaccacaa
    18661 actcttttcc ggaacgggca agcaccaggg taaatgcatc gccggttttg tcttcgatag
    18721 caaattgctc aaagtgcagt gtatcggcgg cgatgtccag acgcgcagct tcacttctta
    18781 ccgcttcaat tagcgcctcg gggccacagg tataaacgtg tgtgccaggt tcgatatccg
    18841 ccaataatcg cgctaattcc agtcgtgttc cggttgatga atgatgcaaa tggactttct
    18901 cagcctgcgg atgctggact agctcagcac gaaatgcgca actttctgga tttcgcgagc
    18961 agtaatgtag ctgccagtcg atgtcgctgt gttgcaactc tgccatgtgc gacaggaaag
    19021 gggggatacc aataccgccc gcgataaaca gatgctttct ggctgaggga attagcgcaa
    19081 acaggttatt aggcgttgag atcgttaacc gatcgcccac ttttacctgc tgatgcaaaa
    19141 agcgggaacc gccgcgcgag ttttcttcca gtcgaacggc aatctgataa ctagaggtgt
    19201 tatgcggtga actcagtagt gaatacgcat tgctgtactg gttatcacca tcgctcatct
    19261 gcactaggat gtgacttcct ccggtgaacg caggcagcgg tttgccatcg gttgccacca
    19321 gcgtgaagcg tttcacctgt tcggtaaggg gttcaacctg gctcacctgt acttcaaaca
    19381 tttgatagtc tgacattgct gtactccatc aaaaatcagc ccagcggcgg aaaataccgc
    19441 cgccgatgtc attagtcctt aaacacctgc gccagcagat tatggaaatg ggcgatacca
    19501 tgttcggaaa tgccactacc gctactgtcg gccatgatgc gcccctgacc acgatagcca
    19561 cgcgatttca gccctttctg tacgctttca accagacgta aatcttccgg atggaacaca
    19621 tcgcgatacc actcaatcag cgatttttgc tcgtcggtta actcttcatt ggtgaagtaa
    19681 atatcgtagt tttgcagggt agtttcagaa tccaccggga attcataaat gaccgtcatc
    19741 atccctttga tcggggtgac gttcagcatc gtgcacggcc acagccagaa accgtggaat
    19801 gccgcatccg taccctcttc aaatttaaac gactgttcgg acggtttggc gaaaccgtat
    19861 tgcagcgtcc agttaccgtg catggtgtgc cagtaacgat caacctgtac ggagtcggag
    19921 aaacctagat gcgccggacc acagtgatag cactcgagat agttatcgac aatgttcttc
    19981 cagttggcag gcgtgcgggt ggtaaagcgg gccgctagtt tcagattgtg gacttccggg
    20041 caggcttcca gcactttcgc gcccagaccg gtgtattgat tcacttgaag tacgaaaaaa
    20101 aaccgggagg acattggatt attcgggttc tgatgggatt agatttggtg gggcttgcaa
    20161 gcctgtagcg caaattttag tcgttaatca atgaaacgcg aaagatagta aaaaattgct
    20221 tttgtttcat tgaaaatacg aaaaacaaaa acactgcaaa tcatttcaat aacagcttca
    20281 aaaaacgttc aaaaccgata acaaccaggc tgtaaccaaa tgactcatat cacaaatcag
    20341 ctcatgccgt ttaggtatgt tacatgtgtg attatgtgag gtgaagtatg ttttagctgg
    20401 ttcatggttg ttatacggct ttttttacat cttgtggttc ttgtgaagga gctacaagac
    20461 cttcctgttc ataaatacca tactttgaca aaatcttttt acgagtatct gttggtaatg
    20521 attcaagcat tttggctgtt tgtaaggttt gctgcgaaat accttgcaaa tcagcctgga
    20581 tggaatcatc ggaagaaaaa atctcaggca taagagcttc aaatcctttc tttctgaaca
    20641 actcaatcgc agaatgcagt tccttgtcct ctaacaattc gacaattgcg atcagtttag
    20701 ttttcaatgt gctgcggtca ggttgagaat ttttattctc ttgattattt ttatgctcaa
    20761 ccattggcat gctctgactt tggataaacc actctattgg acgccctgtt gcgtaggcta
    20821 tttgtgctaa tcgatctaaa gacgggtatg tcttgccaga caggtagttt ctgataaccc
    20881 catcagaaag tccgcactta gctgcaaacg cccggcttga ttctccattc attgcctctt
    20941 caaggcgttc cttgaaacgc attatttccg ggggcataaa aatgcgctct gcatttttgc
    21001 tttctttttc acctgtcatg cccaatctct ctgttatata aagaatttta cagaattctt
    21061 tgatttgcac tggttaaaat aaaaatgagc atagctcttg aattgagcat ttttctgcgg
    21121 tatcgttttt cccgatagcg gtagccggta tcagttaccg aaaacgttaa gagagtaagg
    21181 atcgcagaat gtcagcaaaa aaccaagact ggcacgcaga gcagataaag gctgctgttc
    21241 gtatgaaggg gaagacacta tctcaattat ccagaga
//
LOCUS       DAEEUE010000066        21255 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.066, whole genome shotgun sequence.
ACCESSION   DAEEUE010000066 DAEEUE010000000
VERSION     DAEEUE010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21255
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.066"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..793
                     /locus_tag="KLK84_002652"
     CDS             <1..793
                     /locus_tag="KLK84_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HBE2009923.1"
                     /translation="VAAQPTIVPQLQQQVTGLTSSLNSQEKKELTHKLESIFNNTQVQ
                     IAVLIVSTTKDETIEQYATRVFDNWRLGDAKRNDGILIVVAWSDRTVRIQVGYGLEEK
                     VTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQYPANPSESESTSSTD
                     HYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDLFLFSDKTFSIAVFS
                     FFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGASGRW"
     gene            complement(853..2151)
                     /gene="eno"
                     /locus_tag="KLK84_002653"
     CDS             complement(853..2151)
                     /gene="eno"
                     /locus_tag="KLK84_002653"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBE2009924.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2239..3876)
                     /gene="pyrG"
                     /locus_tag="KLK84_002654"
     CDS             complement(2239..3876)
                     /gene="pyrG"
                     /locus_tag="KLK84_002654"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HBE2009925.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4104..4895)
                     /gene="mazG"
                     /locus_tag="KLK84_002655"
     CDS             complement(4104..4895)
                     /gene="mazG"
                     /locus_tag="KLK84_002655"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBE2009926.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4991..7225)
                     /locus_tag="KLK84_002656"
     CDS             complement(4991..7225)
                     /locus_tag="KLK84_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBE2009927.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7273..8574)
                     /gene="rlmD"
                     /locus_tag="KLK84_002657"
     CDS             complement(7273..8574)
                     /gene="rlmD"
                     /locus_tag="KLK84_002657"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBE2009928.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8631..11387
                     /gene="barA"
                     /locus_tag="KLK84_002658"
     CDS             8631..11387
                     /gene="barA"
                     /locus_tag="KLK84_002658"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HBE2009929.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11619..12959)
                     /gene="gudD"
                     /locus_tag="KLK84_002659"
     CDS             complement(11619..12959)
                     /gene="gudD"
                     /locus_tag="KLK84_002659"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBE2009930.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12980..14320)
                     /locus_tag="KLK84_002660"
     CDS             complement(12980..14320)
                     /locus_tag="KLK84_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBE2009931.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14322..15674)
                     /gene="gudP"
                     /locus_tag="KLK84_002661"
     CDS             complement(14322..15674)
                     /gene="gudP"
                     /locus_tag="KLK84_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HBE2009932.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16108..16557)
                     /locus_tag="KLK84_002662"
     CDS             complement(16108..16557)
                     /locus_tag="KLK84_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBE2009933.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16575..17357)
                     /gene="truC"
                     /locus_tag="KLK84_002663"
     CDS             complement(16575..17357)
                     /gene="truC"
                     /locus_tag="KLK84_002663"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HBE2009934.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRYLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17357..17686)
                     /locus_tag="KLK84_002664"
     CDS             complement(17357..17686)
                     /locus_tag="KLK84_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HBE2009935.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18308..18853)
                     /gene="syd"
                     /locus_tag="KLK84_002665"
     CDS             complement(18308..18853)
                     /gene="syd"
                     /locus_tag="KLK84_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HBE2009936.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18921..19769
                     /gene="queF"
                     /locus_tag="KLK84_002666"
     CDS             18921..19769
                     /gene="queF"
                     /locus_tag="KLK84_002666"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HBE2009937.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19881..21245
                     /gene="ppnN"
                     /locus_tag="KLK84_002667"
                     /gene_synonym="ygdH"
     CDS             19881..21245
                     /gene="ppnN"
                     /locus_tag="KLK84_002667"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HBE2009938.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5226 a         5670 c         5073 g         5286 t
ORIGIN      
        1 tgttgcagcc caaccaacta ttgtcccaca attacagcaa caagttacgg gtcttacgag
       61 tagcttaaac tcacaagaaa agaaagaact gacgcacaag ttagaatcta ttttcaataa
      121 tacccaagtg caaattgctg tattaattgt ttccacaacc aaagatgaga caattgaaca
      181 atatgctaca agagtttttg acaattggcg tttaggagat gccaaacgta atgatgggat
      241 actgatcgtt gttgcctggt cggatcgcac tgtccgcatc caggtaggtt atgggctgga
      301 agaaaaggta accgatgctc tggctggaga tatcatccgt agcaacatga tacccgcatt
      361 taaacaacaa aaattagcta agggattaga gttagctata atcgctttga ataaccaact
      421 cacttcacaa caccaatatc cggctaatcc ttcagaaagt gaatcaacgt cttccactga
      481 tcattattac tttgctattt tttgggtatt tgcagtgatg ttcttccctt tctggttttt
      541 tcatcaaggc agtaattttt gtcgcgcatg taaaagtgga gtctgtattt cagcaatcca
      601 tcttttagat ttattcctgt tctccgataa aactttttcc attgctgtat tttccttctt
      661 ttttactttc accatattta tggtctttac ctgtttatgt gtacttcaga aaagagcatc
      721 tggtagaagt tatcattcag acaacagcgg ttccgctgga ggctcaagtg gcggcgcatc
      781 cggccgctgg taactccacc ataaaaaatg ccagcccgaa ggctggcatt atcaaatcag
      841 ataaagtcag acttatgcct ggcctttgat ctctttacga ccgttgtacg gtgctttttc
      901 gcccagagct tcttcgatac gaatcagctg gttgtattta gcaacacggt cagaacggct
      961 catagaacca gttttgatct ggcctgcagc agtaccaaca gccagatcag cgatggtagc
     1021 atcttcagtt tcaccagaac ggtgagagat aactgcagtg tagccagcat ctttcgccat
     1081 cttgattgca gccagagttt cggtcagaga accgatctgg ttgaatttga tcaggatgga
     1141 gttagcgata cctttttcga taccttcttt caggatcttg gtgttggtta cgaacaggtc
     1201 gtcaccaacc agctggattt tgtcgcccag aactttggtc tggtatgcga aaccgtccca
     1261 gtcagattcg tccagaccgt cttcgataga aacgatcggg tactgtttgg tcagttcttc
     1321 caggaagtga gtgaattctt cagaggtgaa cgctttgttg ccttcgccag ccagaacgta
     1381 tttaccatct ttgtagaatt cagaagctgc gcagtccatc gccagagtga tgtctttgcc
     1441 cagttcataa ccagcagctt taacagcttc agcgataaca gccagagctt cagcgttgga
     1501 acccaggttc ggcgcatagc caccttcgtc accaacagca gtgttcatgc ctttcgcttt
     1561 cagaactttt gccaggtgat ggaaaacttc agaacccatg cggatggctt ctttcacagt
     1621 tttcgcgcca accggctgaa tcatgaattc ctggatatca acgttgttgt cagcgtgctc
     1681 accaccgttg atgatgttca tcatcggaac cggcatggag tatttgcccg gagtgccgtt
     1741 cagttcagcg atgtgctcgt acagcggcat acctttagca gctgcagcag ctttggcgtt
     1801 agccagagat acagccagga ttgcgttcgc gccgaatttg gatttgtttt cggtgccgtc
     1861 caggtcgatc atgatcttgt caatgccagc ctgatcttta gcatctttgc caatcagcgc
     1921 ctgagcgatc gggccgttta ccgcagcaac agctttggtt acgcctttac ccaggaaacg
     1981 ggatttgtcg ccatcgcgca gttccagagc ttcacgggaa ccagtagaag cacctgacgg
     2041 agcagctgcc atgccgacga aaccaccctc cagatgtact tcggcttcaa cagtcgggtt
     2101 accacgggag tcgatgattt cacgaccgat gatttttacg attttggaca ttaggttttc
     2161 ctcaagtcac tagttaaact gaaactccag acaaacaacg cgtacccaag gtacgcgttg
     2221 ccgttctaac ttttttactt acttcgcctg acgtttctgg aactcgctgg cggctttcac
     2281 aaagcctgca aacagcgggt gaccatcacg tggagtagaa gtaaactccg gatggaactg
     2341 gcaagccacg aaccacgggt gattcggaac ttcgatgatc tcgaccaact gatcatcccc
     2401 ggaacggccc gcaacgcgca gacctgcatc ttcaatctgt ttcaacagca tgttgttgac
     2461 ttcgtaacgg tgacgatgac gctcaacaat tgtcggcgca ttgtatagct ggcgaaccag
     2521 gctatcgtca accaactggc actgctgtgc gccgagacgc atggtaccgc cgagatcgct
     2581 cttctcgcta cgaacttcaa cgttgccgtt ttcatcgcgc cactcggtaa tcagcgccac
     2641 aaccgggtac ttacagtctg gcacaaattc tgtagagttg gcgttctcca tgttggcaac
     2701 atggcgagcg taatcaatta acgccacctg catacccagg caaatgccca gataaggaat
     2761 attgttctca cgcgcaaaac gcgcggtcgt aatcatgcct tctacgccac gatagccgaa
     2821 accgccaggt acgaggattg cgtccagacc tttaaggatt tcaacgccgc gcgtttcaac
     2881 atcttgtgaa tcgatcagtt tgatgttgac gctgacacga ttcttcagcc caccgtgttt
     2941 cagtgcttcg atcactgatt tataagcatc cggcagttca atgtacttgc cgaccatacc
     3001 gatggtgact tcacttaccg gattcgcttc ttcgaagata acctgttccc attcggacag
     3061 atttgcttcc gggcagttta agctgaatcg tttacaaata taatcgtcca gcccctgaga
     3121 tttcaacagg cccggaattt tatagatgga atcgacgtct ttcagagaaa taaccgcttt
     3181 ttccggaaca ttacagaaca atgcaatctt cgcacgttcg ttcgccggta cagcgcgatc
     3241 tgaacgacaa atcaggatgt caggctggat accgatggag agcagctctt ttacagagtg
     3301 ctgagtcggt ttggttttga cttcaccaga cgctgccatg tacggcacca gcgtcaggtg
     3361 cataaacaga gtgtgctcac ggccaatttc aacagccatc tggcgaatag cttcgaggaa
     3421 cggcaaggat tcgatatcac ctactgtacc gccgatttct accagtacta cgtcatgacc
     3481 ttcgccacct tccagcacgc gctctttgat tgcgttagtg atgtgcggaa taacctgcac
     3541 ggttgcgccg aggtagtcac cgcggcgttc tttacgcaga acgtcagagt agatacgacc
     3601 cgtggtgaag ttgttgcggc ggctcatttt ggtacgaatg aaacgctcgt agtgccccag
     3661 gtccaagtcg gtttcagcgc cgtcttcagt aacgaacact tccccgtgtt ggattgggct
     3721 catagtacct ggatcgacgt tgatgtaagg atccagtttc atgatggtca cattgaggcc
     3781 acgggcttca agaatggctg cgagggaggc tgcggcaatg cctttaccca gagaggatac
     3841 gaccccgccg gtcacaaaaa tatagttcgt tgtcatgctg aacctgagaa gttaggttga
     3901 aaagacgatg gaataaccaa gacgggaaag cagtataccc gaacatgacc tgtgccacaa
     3961 actttcatta tccctcctct tcgccagcgc actattgaaa tcaggagtga gaaaatagcc
     4021 cctttggggt aaatgttttt gacgcaaatc aagcgcttgt catttaaaaa atgacacaaa
     4081 tggcgcttga ccgcgtaatt cccttagaga tcaatttcct gccgttttac ctgttgccag
     4141 acttcttcca ttgtttcgag gtcaacacct gtcatttcca gtccacgcgc ggcaacaata
     4201 cgctccactt cgcgaaaacg acgctcgaat ttttcgttca ctttttgcaa tgcgatttct
     4261 gctttcgtcc ctaaatggcg agccagatta accgtggcaa acagcaggtc ccccatttcc
     4321 tcctccagtt tagcctggtc gacaacagcc tgccgcgcct cgtacatcac ctcgtcgatc
     4381 tcttcgtaga ctttatcaac caccgggcca agcgtcgtcc agtcgaagcc aacgttggcg
     4441 caacgtttct ggattttttg cgcccgcatt aaagccggta aactacgagg aatatcgtcc
     4501 agcgccgaat gctgcgcttt ctgcgcgcgc tcttcggttt tgatttgctc ccaacgggca
     4561 agcacttcac tactgttttc ggcagaacta tcagcaaaaa catgcggatg gcgacgctct
     4621 aatttatcgc taatagcagc gcaaatatca ttaaagtcaa agcgcccttc ttcctgagcc
     4681 atttgcgcgt aaaacaccac ctggaatagc agatcgccca gttcaccgcg aagatcgtca
     4741 aaatcttcac gggcgatggc gtccagcact tcgtaggttt cttcaagggt gtaaggcgca
     4801 atggtggcaa atgtctgctc tttatcccac gggcagccgt tttccggatc gcgcaggcgc
     4861 tgcataatag tgagcaaacg gtcgatttga ttcattgaat tgtcctgaaa attgcgggtc
     4921 tgtcaggtgt aataccttcg agtaaaagat atcgctggat acggctgacg ccatatccag
     4981 ctatgtaaga ctaactcccg tgcaaccgac gcgcgtcgat aacatccggc acctggttga
     5041 gtttacccag cacgcgcccc agcacttgca ggttgtaaat ctcaatggtc atgtcgatgg
     5101 tcgccagttg ctgtttggtg tcgctacggc tggcaacgcc aagcacgttc accttctcgt
     5161 tggcgagaat ggtcgtgata tcacgtaaca acccactacg atcattagcc accacgcgga
     5221 ccaccagcga atatccggcg gagtagctct caccccatac cgcgtcaaca atgcgttctg
     5281 gcgcatggga gcgcagttcc gccagttgtt cgcaatcggc gcggtgtact gaaataccgc
     5341 gtccctgggt aatgaagccg acaatctcat ctccaggaat cggctggcag cagcgcgcga
     5401 tgtggtgcat caggttacca acaccttcaa ccaccacacg accgttatct ttactgcggt
     5461 tttgcggtgt gtagcttttt tgctgaagct gtttcagcgc ggcggcgtcc tgctcttcgg
     5521 cactcggctt attaaattgc gattgcagga agttcaccat ctgattgaga cggatatccc
     5581 cgccaccaat cgccgccagc aactcgtcga catcattgaa gttgtaacgc ggcagcagat
     5641 gtttttctgc ttctttcagg ctgatcccca gatgttccag ctcgtcgtca aggatttgcc
     5701 gcccagccag aatgtttttg tcacggtcct gtttacggaa ccaggcgtga attttcgaac
     5761 gcccacggct ggttgtgacg taaccgaggt ttgggtttaa ccagtcacgg ctggggttcg
     5821 gctgtttctg ggtgataatt tcaatctggt cgcccatttg cagctggtag gtgaacggca
     5881 caatgcgccc gccaattttt gccccgatgc agcggtgtcc gacatcactg tggatgtggt
     5941 aagcgaagtc cagcggcgtt gatcccgcag gcaaatcaac gacatcacct ttcggcgtaa
     6001 agacatacac ccggtcgtca aacacctgac tacgtacttc gtcgagcatt tcgccggaat
     6061 cagccatctc ttcctgccac gcaatcagtt tacgcagcca ggcaatccgg tcttcatgtc
     6121 ccgaacgtgc gccgccagct gccgcgccct ctttatattt ccagtgcgca gcaacaccca
     6181 actccgcatc ttcatgcatc tgtttggtgc ggatttggat ctcaacggtt tttccacccg
     6241 gccccagaac cacggtatga atagactgat aaccgtttgg tttcgggtta gcgacgtaat
     6301 cgtcaaactc atccggcagg tggcgatagt gagtgtgcac tatccccagt gcggcataac
     6361 aatcctgtaa acgctcggcg acaatacgta ccgcacgcac atcaaacagc tcatcgaagg
     6421 cgaggttctt tttctgcatt ttgcgccaga tgctgtagat gtgtttcgga cgaccataca
     6481 cttcagcttt aacgccttca gctttcatct cagcgcgcag atggccgaca aactcttcga
     6541 tatagtgttc gcggtcgaga cggcgttcat gcagcagttt tgcgatgcgt ttgtattcgg
     6601 tcgggtggag ataacggaag cagtaatctt ccagctccca tttcagttgc ccaatcccaa
     6661 gacggttcgc caacggcgcg tagatattgg tgcactcttt tgccgccagt acacgttcat
     6721 cttccggcgc atcttttact tcgcgcagat gagcaatacg ctccgccagt ttgatgacta
     6781 cgcagcgaaa atcatcgacc atcgccaata acatccggcg aacgttatcg acctgttcgg
     6841 aggaaacaga atcagtgtgc gtcgctttca gctggcggat cgccgccata tcacgcacgc
     6901 cgtgaataag gttaacaacc gacttaccga cgctctcacg cagcacatct tcgctgacta
     6961 cgttggcatc agccagaggg aacagcagcg ccgcccgcag cgtgtcaatg tccatactta
     7021 atgtcgagag gatctccacc atctcaacac cacgccacaa taacagactg gcatccggat
     7081 gcccctgcgt ctgttgcaga caatacgccc aggtttcggc taagcactca cacgacttct
     7141 ggctggtaat acccagactt gcgatccatt tttccggatc aaattcacca gccttattga
     7201 tatgtgcact tcttaccgca accatcgtcc tctcctttag ggaccagacc tgccgaaatc
     7261 ggcaaatcgt aactatttaa cgcgcgagaa aagtaccatc gattccagat gtcccgtgtg
     7321 tgggaacata tccagcatcg ccagtcgcgc aatggtatat cctgctttta ataacgcttc
     7381 gctatcccga gccagcgttg cagggttaca ggatacataa actatacgaa taggttccag
     7441 ttttataatt tgctgcataa cacctgcggc acctgctcgc gccgggtcca gcaacacttt
     7501 atcgaagccg tttttcgccc acggctgctt tgtgacatct tcttcaagat tttcgtgata
     7561 aaacgtcaca ttctgtaagc cattaagacg cgcattctgc tggccttttt ccaccagcgc
     7621 aggaacacct tctacaccga ccacactggc agcttgtgtc gccaatggca gtgtaaagtt
     7681 gcccataccg cagaacagat ccagtacgcg atcttcaggt tgcacatcca gccattccaa
     7741 cgcacgcgct accatttttt ggttcacacc cgcattgacc tgaataaaat cgcgcgggct
     7801 aaaagttaag cgcaacccgt ttgagtcata ccagggcatc tcaccagaga cggtttcgag
     7861 tatctcacta tcgggggcga gatacagatc caggccttca gaatgcgaaa agcgttccag
     7921 tttttcgcga tctgccgaac ttagcggtgc ggtatggcgc aaaatcatca gcgtgccgct
     7981 ggttgcctgt accagttcaa catgaccaag atggcgcata gcttgtaagc tgcccagaca
     8041 tgccctgact ttgggcagca atgcttcaag ttggggcgct aaaatggggc attgtttgac
     8101 gtcgacaatg tcactggagc ccgctttgcg aaaccccatc tgaagttgct gtgttttcgg
     8161 taagtagttc agacttaaac gcgcgcggcg gcgatagccc cagggaacat cggcgatcac
     8221 ttcagagaca tcgtgtttca ttaatcgggc gagtgccgca cttttgcttc gctgctgtaa
     8281 atccacgctg gcgtgttgtt gctgacagcc gccgcatacg ccaaaatgag gacagcgtgg
     8341 cgtttcgcgt tccgggctat cgcttaaccg gcgtacgact ttagcgcggg catactgttt
     8401 tttatcttca gtaacagcaa cttccgcgtt ttcctgcggc aataatccgg ggataaataa
     8461 cgttttgccg ttatgtcgcg ccacgccctg accaaaagag tcgaggtcgt tgactgaaac
     8521 ggttatgatc tgacgcgtcg tcgtgcgtcg ttttgcagag tagaattgcg ccattggcga
     8581 gactttctca atttaacagt gtgaccttaa ttgtcccata acggaactcc atgaccaact
     8641 acagcctgcg cgcacgcatg atgattctga tcctggcacc gaccgtcctt attggtttat
     8701 tgctgagtat ctttttcgtc gtgcatcgct ataacgactt gcagcgtcaa ctggaagatg
     8761 ccggtgctag cattattgag ccgcttgcag tttctactga atatggcatg agcctgcaaa
     8821 atcgcgaatc tatcggtcag ttaataagcg tactgcatcg tcgccattcc gatattgttc
     8881 gcgcgatttc ggtttatgat gaaaataacc gactctttgt cacctccaat tttcatcttg
     8941 atccctcatc aatgcagctc ggcagcaacg tgccgtttcc tcgccagctc actgtcactc
     9001 gtgacggcga tattatgatc ctccgcacgc cgattatttc tgagagttac tcccccgacg
     9061 aatcgcccag tagcgatgcc aaaaatagtc aaaatatgtt gggatatatt gcgcttgagc
     9121 tggatcttaa atcggttcgc ttgcagcaat ataaagagat ctttatttcc tgcgtgatga
     9181 tgctgttttg tatcggtatt gcgcttattt ttggctggcg cttaatgcgc gatgtaaccg
     9241 gtccgattcg caacatggtg aataccgtcg accgcatccg tcgcgggcaa ctcgacagcc
     9301 gggtggaagg atttatgctc ggcgagctgg atatgctgaa aaacggtatc aactcgatgg
     9361 caatgtcgct ggctgcttat cacgaagaga tgcagcacaa tatcgaccag gcgacgtccg
     9421 atctgcgtga aacgctggag cagatggaaa ttcagaacgt tgagttggat ctggcgaaaa
     9481 agcgcgccca ggaagcggcg cgtattaaat ccgagtttct ggcaaatatg tcacacgagc
     9541 tgcgtacacc actgaatggt gttattggct ttacccgcct gacgctgaaa acagaattaa
     9601 caccaacgca gcgcgatcac ctgaatacga ttgaacgttc ggcaaataat ttgctggcaa
     9661 ttattaatga tgttctcgac ttctcgaaac tggaagcagg taagctgatt ctggaaagta
     9721 ttccattccc actacgcagc acgctggatg aagtcgttac tctgctggca cattcttctc
     9781 acgataaagg gttggaactg acgctcaata ttaaaagcga cgtgcctgat aacgtgatcg
     9841 gcgacccact gcgattacag caaatcatca ctaacctggt ggggaatgca attaaattca
     9901 ccgagaatgg caacattgat attctggtag aaaaacgtgc gctgagtaat accaaagtgc
     9961 agattgaagt gcagattcgg gataccggca ttggtattcc tgaacgcgat caatcgcgct
    10021 tattccaggc cttccgacag gctgatgcca gtatttcccg ccgtcatggt ggcaccgggc
    10081 tggggctggt gattacacaa aaactggtta atgaaatggg cggcgatatt tcgttccata
    10141 gccagccgaa tcgcggttca actttctggt tccacattaa tctcgatctg aacccgaaca
    10201 ttattatcga agggccatcc atccagtgcc tcgcaggtaa gcgcctggcc tatgtcgaac
    10261 caaactccgc agcagcgcaa tgcacgctgg atattttaag tgaaacgccg ctggaagtgg
    10321 tttatagccc aacgttctcc gcgctgcctc ccgcgcatta cgacatgatg ttgttaggca
    10381 tcgcggtgac cttccgcgag ccgctaacaa tgcaacatga gcgattagcg aaagcggtat
    10441 cgatgaccga tttcctgatg ctggcacttc cttgccatgc acaagttaat gctgaaaaac
    10501 tcaagcaaga tggtatcggc gcgtgtctgc tgaaaccatt aacacctacg cgcctgttgc
    10561 ctgccctgac ggaattttgt catcacaaac aaaacacgct tttgcctgta accgatgaaa
    10621 gtaagctggc aatgacagtc atggcggttg atgacaaccc cgctaacctg aaacttatcg
    10681 gcgcattgct ggaagatatg gtgcaacatg tggaactttg cgatagcggg catcaggcag
    10741 ttgaacgggc gaaacagatg ccgttcgatt tgatcttaat ggatattcaa atgccggaca
    10801 tggatggcat tcgggcctgc gaactcatcc accaactccc gcatcagcga caaacgccgg
    10861 ttatcgcggt aacggcgcat gcaatggccg ggcaaaaaga gaagctgctt ggcgcaggga
    10921 tgagcgatta tctggcgaaa ccgattgaag aagagcgatt gcataatttg ttgttgcgct
    10981 acaagcctgg cagcggtatt tcgtctcgcg tcgtgacgcc cgaagtcaac gaaattgtgg
    11041 tgaacccgaa tgcgaccctc gactggcaac tggcactacg ccaggcagca ggaaaaaccg
    11101 atttagcgcg cgatatgctg caaatgttac tcgatttcct gcctgaagtt cgcaacaaag
    11161 ttgaggaaca gctggctgga gaaaacccgg aaggcctggt tgatttgatt cataaactgc
    11221 atggcagttg cggctatagc ggcgtaccac gtatgaagaa tctctgccag ctgatcgaac
    11281 aacagctacg tagcggtact aaagaagaag atttggagcc ggagctgctg gaactgttgg
    11341 acgagatgga taatgtcgcg cgcgaagcca gcaaaattct cgggtaacgg acgtcttgtc
    11401 gagtagcaat cggcttttta tgacgctggt ttcagattgg tgacaaagtg cctgtgttta
    11461 tgccggatgc ggcgttaacg ccttatccgg cctacaaaac caattaaatt caatgaatta
    11521 aaaaatgatg tagacctgat acatagcaca acaggctatt ttgcgtttag catcagtctc
    11581 aaaccggctc cagatagagc cggttttggt tttctgtctt aacgcaccat gcacgggcgc
    11641 ttgttatcga acgtccagcc aggaatcaga tactgcattc ccatcgcatc gtcacgcgcg
    11701 ccaagcccgt gtttctgata cagctcatgg gctttcatca cttgatccat atcgatttct
    11761 acacccagcc ccggtttttc tggcacctgt accagcccgc ctttgatctc aaacggttct
    11821 ttggtcaggc gctgattacc ttcctgccag atccagtgcg tatcaatagc agtaatttta
    11881 cccggtgcag cggcggcaac atgggtaaac atcgccaggg aaatatcgaa gtggttgttt
    11941 gagtgtgaac cccaggtcag gccaaattca tggcacattt gcgccacacg taccgaacct
    12001 tgcatagtcc agaaatgcgg atccgccagc gggatatcaa cggattgtaa cgagagcgta
    12061 tggcccatct gccgccagtc ggtggcgatc atattggttg cagtcggcag gccagtcgcg
    12121 cgacggaact ctgccatcac ttcacgcccg gagaaacctt gctccgcacc acacggatct
    12181 tctgcataag ccagcgaacc tttcaggtat ttaccaattt taattgcttc gttcagcgac
    12241 caggcaccgt taggatcgag cgtaatacgc gcctgcggga agcgttgcgc cagtgccaca
    12301 atagactcgg cttcttcttc cccggccagt acaccgcctt tcagtttgaa atcgttgaag
    12361 ccatattttt catacgccgc ttccgccagg cgcaccaccg catccggcgt catcgcttct
    12421 tcatgacgca ggcgatacca gtcgcatgag tcatccggct ggctttgata cggcagaggt
    12481 gtggctttgc gattaccgac gaagaacaga taaccgagca tttcgacttc gctacgctgt
    12541 tgaccatcgc ccagcagcga tgccacgttt acccccagat gctgccccag cagatccagc
    12601 attgccgctt ctatcccggt aactacatga atagtggtac gcaaatcaaa tgtctgcaaa
    12661 ccgcgcccac tagcatcacg atcggcaaaa gtattacgca ccagcgtcag aacgtttttg
    12721 tattcaccca gcgttttacc taccaccagc ggaatcgcat cttccagcgt tttacggatt
    12781 ttctcgccgc cgggaatttc ccctacgcca gtgtgaccgg aattatcttt gataatcaca
    12841 atattacgcg tgaagaacgg tgcgtgtgca ccactcagat tcatcagcat actgtcatga
    12901 cccgccaccg ggatgacctg catttcagta acaacaggcg tcgtaaattg agaactcata
    12961 tttatgtcct ttttcagaat caatgacggc cgaaaacggg acgtttacgg tcaaaggtcc
    13021 agccggggat caggtactgc atcggacctg cgtcgttacg cgcaccgcaa ggcagacgtt
    13081 tataggcctc atgtgccttt tgtacctgtt cccagtccag ttccacgccc agaccgggcg
    13141 catcaggaac ggcaattttt ccgtttttaa tctccagcgg atttttggtc aggcgacaat
    13201 cgccctcctg ccaaatccag tgggtatcga tagcggtagg attacccggt gccgccgcgc
    13261 ccacatgggt aaatatcgcc agagagatat cgaaatggtt gttggaatgg cagccccagg
    13321 tcagccccca gtcgtcgcaa agctgcgcca cgcggactgc accggaaagc gtccagaagt
    13381 gcggatcggc aagtggaata tctaccgcat tgagcatcac cgcatgaccc atttcgcgcc
    13441 agttggtggc gatcatgtta gtcgcgacgg gcaagccggt cgcccgtcga aactccgcca
    13501 tcacttcacg tccggagaag ccctgttctg cgccgcatgg atcttcggca taggtaagaa
    13561 catcattcag ccctttgcat aaagaaatgg cttcatcaag cagccatgca ccgttgggat
    13621 caacggtaat ccgcgcatcc gggaagcttt tcttcaatgc acgaacagtg tcgatttctt
    13681 gctcgccagg taacaccccg cccttaagtt tgaaatcttt aaagccatag cgatcctgtg
    13741 aggcttccgc cagacgcaca acggcttcgc tgttcatcgc tttctgatgg cgcaactgat
    13801 accactcatg gttgcccggc gtattttcca gataaggaag atcggttttg gtccgatcac
    13861 ctatataaaa cagataaccg aggacggtaa tagtctcgcg ttgcttgcct ggccccaaca
    13921 gttcgcagac cggaacattc agcgccttac ctagcaggtc aagcaaggcg gcttccagcg
    13981 ccgccacggc attaacgcgc aattcaaaag tccaggcacc tttgccgaag gtatcaaaat
    14041 cggctgcctg attaccttta tgtacctgct gaaccacttt attcaggcgc gcaacttcct
    14101 ggcccagaac catcggaata gcatcgacca gtgtttgata aatcacctct ccgcccggcg
    14161 cttcaccaat gccggtatgc ccggcgttat cggtgagtac cacgatattg cgggtgaagt
    14221 atgcgttatg cgcaccacca atattgagca acatgctgtc atgtccggcg accggaatga
    14281 ctttcatatc agtaataaca ggactggatt gtgtcgccat cttattgccc cgcgacaggt
    14341 ttcagctcga tacgcttgat atcgcccacc agcaccaggt agctcagtac cgcgattagg
    14401 gcatgaacac caacataaat cagcgcccca ttaaacgagc cagtcgtgcc aacgatataa
    14461 ccaattgcga ttggcgtgac gatgccagaa atgttaccga acatgttgaa caggccaccg
    14521 gaaagaccgc tgatctcttt tggcgcggta tctgccatta ctgcccaacc cagcgccccg
    14581 atgcccttac cgaagaaggc cagcgccata aagccgatga tcatccactc aacgttgacg
    14641 tagttgcaga acaccatcac catcgacagc aacatgccca ttacgatcgg tgttttacgc
    14701 gcaatgttca gcgatcccgt gcggcgcatc agccagtcgg aaataattcc acccagcaca
    14761 ccgccgataa aaccgcaaac cgccggaacg gaagccacaa agcccgcttt cagaatcgac
    14821 atcccacgcg cctgcaccag ataaaccgga aaccaggtaa tgaaaaagta agtcagggcg
    14881 ttgatacagt actgaccgat ataaacgccg atcatcatcc gtgacccgag cagctgtttg
    14941 atctgccccc acttcacgct gaacggcact ttaactttgg tgctttgctg atccatattg
    15001 atcagcgcgc cacccgcggc gatatactcc agctctttct gatttacccc cggatgttga
    15061 tttggctcgt ggatgacttt caaccagata aagctgatga caatgcccag accgcccata
    15121 aagaagaaga cgtgtgacca gcccacttca tgcgtcagcc agcccataat cggcgcgaag
    15181 atcaccgttg cgaagtattg agcggagtta aaaatcgaca ccgccgttcc cctttcctgc
    15241 accggaaacc aggccgcaac aatgcgactg ttgccgggga aagatggcgc ttcagcaagc
    15301 ccgaccagga agcgcagagt aaacagggca acgataatgc cgaatccact aaagatatcg
    15361 acgaagcctt gcagcaaggt aaacatcgac cagataaaga tcgaccagaa gtagacgcgt
    15421 tttgaaccaa aacgatccag caaccaacca ccagggatct gcccgataac ataagcccat
    15481 gagaaggcag agaacacata gcccattccc acgggatcaa ggccgatatc tttggccatt
    15541 tccgaaccgg caatagaaag cgtagcgcgg tcgccgtagt tgaaggatgt gacgataaac
    15601 aacatcacca ctatccagta acgagcattc gtgcgttttt ccacactgct cgcagcctga
    15661 cttaaagaac tcattgttgc actcctgaaa attcgcgtta gccacgctca ctctggactg
    15721 cgacatcgcc aggaaatcag aggtgacgta gggtgttttt tgccgttttt ataggtcgtt
    15781 cgccgaatac ggcgcgtgtt tatatctggc aatagcagta taaaaagcgc gccatagcgg
    15841 ctcaccgtgc aacaacacaa cattaatgcg ttcaatgagg cccgattttg gcattagccc
    15901 tggacggtgg aatccacttc acggaaatga aaacaagaac aagaaaggaa gggtaaaaac
    15961 gaagaaataa agaagagtat gaaatggatc gcttgactct aggcaaacgc cagtaaaaat
    16021 ccgcgctatg aagcagtttt tactggcatt tgcctgaaaa gattcgattc agcaccgcta
    16081 aaacgacatt taccgctcgc tgacatatca ggacaacagc gtgccccact gttcgaccca
    16141 cggatttgat tccgtttccg gttccgggtt ctcactggcg tcaatcaaca gcatttcgcc
    16201 aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac tgtttgccgc cattgcaaaa
    16261 atttacataa ctgctgtcgc cgagcgcaat cacgccataa cgcagattcg gctggaagcc
    16321 cagactatct ttgattccct gaaagagcgg cacaatgcta tcaggaaggt cgccctgccc
    16381 ggtcgtggac gtaaccacca gaacatactt atcctgatag ggcagccagt cgcttaattc
    16441 aggatcttca aataccgttg ctttgtggcc ctgtgcggtc agaattgctt ctgcttcttc
    16501 cgccactaac agcgaatttc cgtacatggt gccgacaaaa ataccaattt ccgccatacc
    16561 gttttctccc tggattagga acttatctct ccatcctgac ccgatggcgc actgaactca
    16621 accctttcat tttcaggaag cagaccgcgc cagccaaatt gtgataacgc ctgcatccag
    16681 gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag taaaaggatg cgtcaatgac
    16741 aactgactgg catgtagcat taaccgctgg aggccaaaat gctcagcacc gctgcgattc
    16801 tggcgtaaat cgccatgttt gctatcgcca ataatcggat gacgcaaatg ggcaagatat
    16861 cggcgaagct gatgtttgcg tccggttttc ggctccagtt ccaccaggcc gtagcgcgtg
    16921 gtcgggtaac gtccggtcgc taccggcatt tctacggtcg ccagaccgcg ataatgcgtc
    16981 actgctggct gcgggccttt atcttcgcgg gcaaatttat cagcgatttt gtccagttct
    17041 tccaccagtg gataatccaa caccgcttct tccatcaacc agccgcgcac aatcgcatgg
    17101 taacgtttct ggatttggtg ctgttcaaac tgttgtgcca gcagccgtcc ggcctcgctg
    17161 gataatccca tcaacaacac accagaagtg ggtcggtcca gacgatgagc agtaaaaaca
    17221 tgctgtccta tctggtcacg cacggtttgc atgaccacta ctttctcgtc gcgatccagc
    17281 cagctacggt gaaccagcca gccggagggt ttatttaccg caaccagcca ttcatcctga
    17341 tagagtattt ccagcattag ctcgcatcat ccgcaaaaag agcatccagt ttttccaact
    17401 cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag cgccatttca taatagggtg
    17461 caacagcaaa agcgcccggt aacggctggt tgttatttaa caaatcgtgc attcgcggga
    17521 tcagccccca ctgcaaccac tccagtggtt ccatggtgtc cataaagaac ggttgggtac
    17581 tattaaattg atgcggctgg ggttcatcgt ttcgccagtg ctgatgttca cgcagtaacg
    17641 cttcaagcgc ctgcaactgg agacgaacgc ggtcatgagt ggtcataaag caacctcaat
    17701 aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg gagcactgta ttcacagtgc
    17761 tcccggttcg tttcgcagca ttccagctac ttttgttgct ccctgctcat ccttgacaac
    17821 ttttcctctg gccttgcggc caatcgttca tcctgaacta ttgcttcctg ctcacaccac
    17881 cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc tgacccaccg aatcatcctg
    17941 accggttctc attctccatc ctggaggtgt cctttaacgc gtcctgcgtc atcctcttcg
    18001 cttcatccag aagcctttcc ctgaaacacc atcctggtgt gtcctgcaga agtgtcatca
    18061 tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa gatagaatcg tctttttcgc
    18121 gaagtcttac aaggcgctta cagacaatgc cttaaggaaa atttcatatg aaagttaaga
    18181 attaacaatc aacattttga ttttaaatga aaataacgat tcttatcttt ttaagtattc
    18241 gcttatttcc tggcgatctc ctacagagtc tgtaagagat ctctcacaac cagatggggt
    18301 atttggatta cagaagaggc ttaagttgat taaggaattc cgcaagattt gaggctaaat
    18361 gcgtccgttt acgtgttccc agcgtctctt tgcacacttc accggagaga ttacacaccg
    18421 agatgacctc taactcttct tccagcgtcg cgataaatag cgtgggcggc agttttagcc
    18481 ttttctgagt caccagatga ccaatcagat tttcctgcac ccgacggaag tcgtcttcac
    18541 tccaggtttg cagcaatgtc agtttgatat cgccaaactg cgcgtgcata tccccggcaa
    18601 actgagtcgt ataaaaggta tgaattgtgg gttgtatcac aatatcaaaa gcgcgttcaa
    18661 ccgcgtttac attttgttcc ccggtaaacg gctggggttg ccagtatacg gcatcttcgg
    18721 tagttgaaat aatgcacggt gaaggaacac cgtacaattc ctcacttaac ggccagcttt
    18781 tatgctcttc atgccacgca tcgcagtagc gtgcagtaaa atctttcagg gcttgtgcgg
    18841 tcaaatcgtc cactaatttc tctcttcact taaaccagat acacttgtcg tttagtttat
    18901 ctggtttatg acggtgaaac atgtcttctt atgcaaacca tcaggcactt gcgggcctga
    18961 ctcttggaaa atcaaccgat taccgggata cctatgacgc cagcctactg caaggcgttc
    19021 cacgcagcct gaatcgcgac ccgctgggtc tgaaagcgga taacctgcct tttcacggta
    19081 cggatatctg gacgctgtat gaactttcct ggctgaatgc gaaaggtttg ccgcaggtcg
    19141 ctgtcggtca tgttgaactt gattacacca gcgtaaatct gattgagtcg aagagtttta
    19201 agctctatct caacagtttt aaccagacgc gttttaataa ctgggatgag gtgcgccaga
    19261 cgctggagcg cgacttaagc acttgcgctc agggtaagat tagcgtggcg ttatatcgtc
    19321 ttgatgaact ggaaggccag ccgataggtc atttcaatgg cacttgcatt gatgaccagg
    19381 atatcactat cgataactat gaattcacta ctgactatct ggagaatgcc accagcggtg
    19441 aaaaagtagt ggaagagacg ctcgtcagcc acctgctgaa atcaaactgc ctgatcaccc
    19501 atcaaccaga ttggggttcg atccaaattc agtatcgtgg acgccaaatt gacagagaaa
    19561 aactgctgcg ttacctggtc tcattccgtc atcacaacga gttccacgaa cagtgcgtgg
    19621 aacgcatctt taatgacctg ttacgcttct gccagccaga gaaattgagc gtttacgcac
    19681 gttatacccg tcgtggcggt ctggacatta acccgtggcg cagtaatagc gattttgtcc
    19741 catcgaccac aagactggtt cggcaataaa ttttttctca attttgcgtg ctggattcac
    19801 gcagaaggtt gtgaaaggtc atcaggcagg gctattgtaa tcaaagggaa tgacgatatt
    19861 cgtcccataa ggagtttttc ttgattacac atattagccc gcttggctcc atggatatgt
    19921 tgtcgcagct ggaagtggat atgcttaaac gcaccgccag cagcgacctc tatcaactgt
    19981 ttcgcaactg ttcacttgcc gtactgaact ccggtagttt gaccgataac agcaaagaat
    20041 tgctgtctcg ttttgaaaat ttcgatatta acgtcctgcg ccgtgaacgc ggcgtaaagc
    20101 tggaactgat taatcccccg gaagaggctt ttgtcgatgg gcgaattatt cgcgctttgc
    20161 aggccaactt gttcgcggtt ctgcgagaca ttctcttcgt ttacgggcaa atccataata
    20221 ccgttcgttt tcccaacctg aatctcgaca actccgtcca catcactaac ctggtctttt
    20281 ccatcttgcg taacgctcgc gcgctgcatg tgggtgaagc gccaaatatg gtggtctgct
    20341 ggggcggtca ctcaattaac gaaaacgagt atttgtatgc ccgtcgcgtc gggaaccagc
    20401 tgggcctgcg tgagctgaat atctgcaccg gctgtggtcc aggagcgatg gaagcgccga
    20461 tgaaaggtgc tgcggtcgga cacgcgcagc agcgttacaa agacagtcgt tttattggta
    20521 tgacagagcc gtcgattatc gccgctgaac cgcctaaccc gctggtcaac gaattgatca
    20581 tcatgccgga tatcgaaaaa cgtctggaag cgtttgtccg tatcgctcac ggcatcatta
    20641 tcttccctgg cggtgtgggt acggcagaag agttgctgta tttgctggga attttaatga
    20701 acccggccaa caaagatcag gttttaccat tgatcctcac cggcccgaaa gagagcgccg
    20761 actacttccg cgtactggac gagtttgtcg tacatacgct gggcgaaaac gcgcgccgcc
    20821 attaccgcat catcattgat gacgccgctg aagtcgctcg tcagatgaaa aaatcgatgc
    20881 cgctggtgaa agaaaatcgc cgtgatacag gcgatgccta cagctttaac tggtcaatgc
    20941 gcattgcgcc agatttgcaa atgccgtttg agccgtctca cgagaatatg gctaatctga
    21001 agctttaccc ggatcaacct gttgaagtgc tggctgccga cctgcgccgt gcgttctccg
    21061 gtattgtggc gggtaacgta aaagaagtcg gtattcgcgc cattgaagag tttggtcctt
    21121 acaaaatcaa cggcgataaa gagattatgc gtcgtatgga cgacctgcta cagggttttg
    21181 ttgcccagca tcgtatgaag ttgccaggct cagcctacat cccttgctac gaaatctgca
    21241 cgtaatctcc cctcc
//
LOCUS       DAEEUE010000067        20907 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.067, whole genome shotgun sequence.
ACCESSION   DAEEUE010000067 DAEEUE010000000
VERSION     DAEEUE010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20907
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.067"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            60..1386
                     /gene="pgaC"
                     /locus_tag="KLK84_002668"
                     /pseudo
     CDS             60..1386
                     /gene="pgaC"
                     /locus_tag="KLK84_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
     gene            1388..1801
                     /gene="pgaD"
                     /locus_tag="KLK84_002669"
                     /pseudo
     CDS             1388..1801
                     /gene="pgaD"
                     /locus_tag="KLK84_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
     gene            complement(1851..2639)
                     /gene="phoH"
                     /locus_tag="KLK84_002670"
     CDS             complement(1851..2639)
                     /gene="phoH"
                     /locus_tag="KLK84_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HBE2009939.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(3247..4512)
                     /gene="efeB"
                     /locus_tag="KLK84_002671"
     CDS             complement(3247..4512)
                     /gene="efeB"
                     /locus_tag="KLK84_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HBE2009940.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(4518..5645)
                     /gene="efeO"
                     /locus_tag="KLK84_002672"
     CDS             complement(4518..5645)
                     /gene="efeO"
                     /locus_tag="KLK84_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HBE2009941.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(5703..6377)
                     /locus_tag="KLK84_002673"
                     /pseudo
     CDS             complement(5703..6377)
                     /locus_tag="KLK84_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
     gene            6466..6831
                     /locus_tag="KLK84_002674"
     CDS             6466..6831
                     /locus_tag="KLK84_002674"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009942.1"
                     /translation="MARSAKPRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDS
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGG
                     MYCPECGTVHWPDGVAPPF"
     gene            6831..8018
                     /locus_tag="KLK84_002675"
     CDS             6831..8018
                     /locus_tag="KLK84_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HBE2009943.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGTFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRSWNVNVSRVMDNATHVAVCFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDESMERFSWHVADKGFRMVRYYVFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
     gene            complement(8312..8950)
                     /gene="rutR"
                     /locus_tag="KLK84_002676"
     CDS             complement(8312..8950)
                     /gene="rutR"
                     /locus_tag="KLK84_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HBE2009944.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQT
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            9238..10329
                     /gene="rutA"
                     /locus_tag="KLK84_002677"
     CDS             9238..10329
                     /gene="rutA"
                     /locus_tag="KLK84_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HBE2009945.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            10329..11021
                     /gene="rutB"
                     /locus_tag="KLK84_002678"
     CDS             10329..11021
                     /gene="rutB"
                     /locus_tag="KLK84_002678"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HBE2009946.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            11033..11419
                     /gene="rutC"
                     /locus_tag="KLK84_002679"
     CDS             11033..11419
                     /gene="rutC"
                     /locus_tag="KLK84_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HBE2009947.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            11427..12227
                     /gene="rutD"
                     /locus_tag="KLK84_002680"
     CDS             11427..12227
                     /gene="rutD"
                     /locus_tag="KLK84_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HBE2009948.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            12237..12827
                     /locus_tag="KLK84_002681"
     CDS             12237..12827
                     /locus_tag="KLK84_002681"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HBE2009949.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            12838..13332
                     /gene="rutF"
                     /locus_tag="KLK84_002682"
     CDS             12838..13332
                     /gene="rutF"
                     /locus_tag="KLK84_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HBE2009950.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            13353..14681
                     /gene="rutG"
                     /locus_tag="KLK84_002683"
     CDS             13353..14681
                     /gene="rutG"
                     /locus_tag="KLK84_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HBE2009951.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(14763..14870)
                     /locus_tag="KLK84_002684"
     CDS             complement(14763..14870)
                     /locus_tag="KLK84_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2009952.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            15243..15839
                     /gene="wrbA"
                     /locus_tag="KLK84_002685"
     CDS             15243..15839
                     /gene="wrbA"
                     /locus_tag="KLK84_002685"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBE2009953.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            15860..16087
                     /locus_tag="KLK84_002686"
     CDS             15860..16087
                     /locus_tag="KLK84_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HBE2009954.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(16125..17366)
                     /gene="agp"
                     /locus_tag="KLK84_002687"
     CDS             complement(16125..17366)
                     /gene="agp"
                     /locus_tag="KLK84_002687"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HBE2009955.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(17659..18915)
                     /locus_tag="KLK84_002688"
     CDS             complement(17659..18915)
                     /locus_tag="KLK84_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HBE2009956.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSEPITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            19176..20096
                     /gene="cbpA"
                     /locus_tag="KLK84_002689"
     CDS             19176..20096
                     /gene="cbpA"
                     /locus_tag="KLK84_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HBE2009957.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            20096..20401
                     /gene="cbpM"
                     /locus_tag="KLK84_002690"
     CDS             20096..20401
                     /gene="cbpM"
                     /locus_tag="KLK84_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HBE2009958.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(20494..>20907)
                     /gene="torD"
                     /locus_tag="KLK84_002691"
     CDS             complement(20494..>20907)
                     /gene="torD"
                     /locus_tag="KLK84_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HBE2009959.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
BASE COUNT         4991 a         5354 c         5278 g         5284 t
ORIGIN      
        1 taaccaacct gaaatagacc ttattcgtcc tgagttttca acagcctggt atccgaaaaa
       61 tgattaatcg catcgtatcg ttttttatat tatgtctggt gttatgcata cccctatgcg
      121 tagcgtactt tcactctggt gaactgatga tgaggttcgt tttcttctgg ccgtttttta
      181 tgtccattat gtggattgtt ggcggcgtct atttctgggt ctatcgtgaa cgccactggc
      241 cgtggggaga aaacgcacca gctccccagt tgaaagataa tccgtctatc tccattatca
      301 ttccctgttt taatgaggag aaaaacgttg aggaaaccat acacgccgct ttagcacagc
      361 gttatgagaa cattgaagtt attgccgtaa atgacggttc aacagataaa acccgtgcca
      421 tcctggatcg catggctgca caaattcccc atttgcgggt cattcatctg gcgcaaaaac
      481 aggggaaagc cattgcgctt aaaaccggag ctgccgcggc gaaaagtgaa tatctggtgt
      541 gcattgatgg cgatgcgtta ttagaccgcg atgcggcggc atatattgtg gaaccgatgt
      601 tgtacaaccc gcgtgtgggt gccgtaaccg gtaatcctcg tattcgaaca cgttctaccc
      661 tggtgggtaa aattcaggtt ggcgagtatt cctcaattat tggtttgatc aagcgaaccc
      721 agcgtatcta tggaaacgta tttaccgttt ccggtgttat tgccgcattt cgtcgcagcg
      781 ccctggcaga agtgggttac tggaatgacg atatgatcac cgaagatatt gatattagct
      841 ggaagctgca gttgaatcag tggccgattt ttttacgagc cacgggcgct gtgctggata
      901 ttaatgcctg aaacgttaaa agggctgtgg aaacagcgcc tgcgctgggc tcagggcggt
      961 gcagaagtat tcctcaaaaa tatgacaagg ttgtggcgca aagaaaactt tcgaatgtgg
     1021 ccgctgtttt ttgaatactg cctgacgaca atatgggcct tcacctgcct ggtcggtttc
     1081 attatttacg cagtccaact tgccggtgta ccgttaaata ttgaattgac acatatcgct
     1141 gcgacacata ctgccggaat attattgtgt acgttatgtt tactgcaatt tattgtcagc
     1201 ctgatgatcg agaatcgcta tgagcataat ctgacttcat cgcttttctg gattatttgg
     1261 ttcccggtta ttttctggat gctgagcctg gcaacgacat tggtatcatt tacacgagtc
     1321 atgttgatgc ctaaaaagca acgcgcccgt tgggtaagtc ctgatcgcgg gattctgaga
     1381 ggttaatatg aacaatttaa ttattacgac ccgacaatca ccagtacgtt tactggttga
     1441 ttatgttgcc acaaccatct tgtagacatt atttgctttg ttcatattct tattcgccat
     1501 ggatctgctg acgggttatt actggcaaag cgaggccaga agccgacttc agttctattt
     1561 tttgctggca gtggcgaatg ccgtcgtgtt aattgtctgg gcgctgtaca ataagctgcg
     1621 ttttcaaaaa cagcagcatc atgcagccta ccaatatacg ccgcaagaat atgcagagag
     1681 cttagcaata cctgatgagc tctatcagca actacaaaaa agccacagga tgagcgtaca
     1741 cttcaccagc caggggcaaa taaaaatggt tgtttcagaa aaagcgctag tccgggcata
     1801 aacacccaaa acaaagcccg gttcgcccgg gctctgcacc gataatacac ttaactgtat
     1861 gcatgcagcg tacgttggca aagtgccgaa cgtacgcaat cctcttttcc gaaccggacg
     1921 atcccaacca tttcatcttc ttcgaaacgt tccagcgcgt cacttaatcc ggagcacacg
     1981 ccgcgaggca aatcgcattg cgtgatatca ccgttgacga taaccgtcac gttctccccg
     2041 aggcgggtta aaaacatttt catttgcgcg gcagtcacat tctgcgcctc gtcaagaatg
     2101 acaactgcat tttcaaaggt acgtccacgc atataggcga acggcgcggt ttccaccttc
     2161 cctatttccg gtcgcaggca gtactgcata aaggaagccc ctaagcgccg gaccagcaag
     2221 tcgtagaccg ggcgaaaata gggagcaaac ttttctgcga tatcaccagg taagaagcca
     2281 agatcttcat cggcttgcag aactggacgg gtgacgataa tcctgtcgac atccttatgt
     2341 atcagggcct ctgccgcttt tgctgcgctg atccaggttt ttccgcaccc ggcttcgccc
     2401 gtggcgaata tcagctgctt actctcaata gcctgcagat agtgcaattg cgcttcattt
     2461 cgcgcaagga tgggcgaggt atcgcgactg tcgcgggcca taccaatggc ttctacgccg
     2521 ctcatctgca caagcgaggt gaccgattct tcttcacgct gcttatggct gcgcgaatcc
     2581 cgtctcagca cacgttttgc ctcgcgacga gctttgatca ctgctttttg tcttcccatg
     2641 gagagcacct tgagttgttt gtattcatca cacgcgccgt tggcagcgcg attatgcgca
     2701 cgaacatcag agggttggct tccttgtaag ccatagtttg cttttggata aaatgccgaa
     2761 aaacggctac gcacaccgtt tgcggcgtcg gtaacacgag taaagaaagg aagtgagaaa
     2821 aatacagagt gacgaagttg ctaccggagg agaaatctcc gcgagtggtg gagcgttgct
     2881 taacaaaaca ttgtccagaa caggacgtta ccatccgcga tctccatagt gactgactat
     2941 cactgccggg aacttccgct gctacttaat aagtacaaca gaactcgcat tcattgcaac
     3001 aatatattca cttgtattta acgataaaca cccatttcgg tgacattaat ttctactgga
     3061 aagatgacag agtgatgaca gtgatggaaa aagctgtgtg ctttccgcag gattcgcagg
     3121 gctggtaaaa tgtgcctcgt gtttgccgga ttcggcgcga acgccttacc cgacctacaa
     3181 gtgattaata atttgtgcac aaaactcatg cccgataaac ctgcgcatcg ggcatatttg
     3241 cgtcgttcaa acctgcaata gcgcccttcc cagataatcg ttcgcgtcct tcactcccgg
     3301 cagcgaaaaa aaataaccgc cgccgatagg tttaacgtat tcctccagcg cttcgccatt
     3361 gagccttttt tgtactgtca ggaagccttt ttccagatcg tgttggtagc agacaaacag
     3421 caaccccata tccagttgtc cggagttggt gacccccaac gaataactgt agccgcgacg
     3481 cagcatcagg ctggactcac tctccgccgt gcggggattc gccagccgga tatggctgtc
     3541 cagcgcgatc accttccctt ccgggtcgct ggcgtaatca ggcacatcat gctcatgctg
     3601 cattcccagc ggcgcaccgg tttgcttatc acggccaaaa atcgtctgct gttctttcag
     3661 cggcgttctg tcccaaaatt ccactcgaaa ctgaatcaag cgtactgcct gatagctgcc
     3721 accgattgtc cacgcaggct cctgctgatc tgccgttacc cacaccactt tttgcatcaa
     3781 cttatcattc tggctatcgg gattggcagt gccgtctttg aaacccagca aattaatcgg
     3841 cgtctctttg cctttactac gcgccgcgtg atcggaaata aacccttccc gcttccagcg
     3901 cacactgagc aaatccggcg tgtgtttgat gatatcgcgc agcgcatggt tcaccgtgtc
     3961 ctgggtgttg gcgcaaatct gtagcaacac atcaccatga cataacgccg catccagcga
     4021 gtcgttgggg aaacgcgtca tcttctgcag cttttttggc atctgtggcg caaggccaaa
     4081 gcgctcatca aacaatgagt gacccaccga taacgtgatg gtgagattat cgggcgcaat
     4141 gtagccgcca agaatgccgg aatcgagtgg tggcaggcgc ggatttggcg tttctggtgc
     4201 tgctccgccc tgactcagaa aagcaaaacg ctgagtcaac aaacgaaaca accgctcaag
     4261 atcggcttta tcgctggcaa gcacatcaaa cgccaccagc atcattgcgg cctgttgtgg
     4321 cgtcaggatc cctgcctgat gctcaccata aaacggctgt ttctcattgc gtgcatccgg
     4381 tgaaagcgtg cccggtgcac tttgcgtttt ttgtgcatga gcgaccggac aacttcccgc
     4441 cagcgcacca atccctttca gtaaacgtcg gcgtgacggt tcattcacgc cgttttcatc
     4501 tttatactgc ataacgctta atccagtccc agcacaccgc gaagttgcgc cagatcttcc
     4561 gccagcgcag taatcggccc tttcagcgca ttccggtcgg catcggtcaa tttgtcgtag
     4621 gtttcaaaac cgtctttagt acggtatttc gccagaatgg tatcgacctt tttaaagttg
     4681 gcatcgactt ttgccaacag ttccgggttg gctttttgca gttgtggacg cagcagatcg
     4741 acaattttct gcgagccttc aacgttagcc tggaaatccc acagatcggt gtggctgtag
     4801 cgatcttctt caccgctgat tttgctggct gccacttcct caatcagtcc ggctgcgccg
     4861 ccgaccactt ttgaaggtgg gaaagccagt tcactgatgc gtttttgcaa atcgaccaca
     4921 tcggtataaa gctgctcagc gtactgatcc atccctttgg tggtgttgtc gccaaacaat
     4981 gctttttcca gacggtggaa accagtgaat tttgggtcgg cggctttttg ctcgtaatca
     5041 tcttcacggg cgtcaatgct gccatccaga tcggagaaca gttcagcaat cggttcaata
     5101 cgctcatagt gctggcgcgt cggcgcatac agtgctttcg ctttttcgat atcgcctgct
     5161 ttaatcgcgt cggtaaaggc tttggtgtcg gtcaccagct gcgtggtttc cgccatgaca
     5221 tacgctttat atgcagtaat tgcaccacca agacttaaca gcgcatcact ttgcgccgca
     5281 tccgccgttg cctcaccttt gacgatcaac ttccctttcg ggttagtcag cagaccgcag
     5341 gtcatatcgt attcgccagg ctgtaaattc gccgtcattt tctggctaaa accaggggcg
     5401 atattttccc gctcttccac caccatcacg cctttgagga tctcccactc cagcgccttc
     5461 tggctatggt tctgaataat gaactgtgtt ttcccggcgt taaccgtaat ggtcatcggt
     5521 tcgcactgct tatccgtcac ggtcactttg acctgcggca catcagcggc gttagccata
     5581 aaagcagaag aaaacagcgc agccacgctc aactgcaatg cgttacggcg gaagttaatg
     5641 gtcatgacga gactatccct ttaaagtatg ttgtaactaa gcaagagttt gcgtcgtatt
     5701 tgttacgcgg agcgagacgc tgtcgcccct gcgcgtggtg gcagagcaaa tgccaccagc
     5761 gccgggatga gataaataaa ccagacggcg acttcgctga cgctcggtgc ttcctgatag
     5821 ccaaaaatcc cttccatcag cgtgccaaac agcgagtgag ttgagaggac cgcgctcata
     5881 tcgaaggcga tttcctgaaa gtggttccac aatccggctt catgaaatgc gcgaatggca
     5941 ccagccgcca gccctgcggc gacgaagaga ataaacaggc tggtccattt aaaaaatgca
     6001 ccaagattga ggcgaatacc gccccagtag agcaggaagc cgagcaccac ggcagtagca
     6061 agaccgagca ttgcacccag cggcggccag atcccgacat cttgttgaaa tgccgccagc
     6121 aggaaaaaga ccgactccag cccttccctt gcaacggcaa aaaagaccat catcaccagc
     6181 gcccagccat gatgatttcc acgctgcaat gcgctatcga ctgcctgttc cagttgcact
     6241 ttgacgttgc gcgacacttt gcgcatccag aaaaccatcc aggtaaggat caccacggcg
     6301 atcaccgcca cgataccttc aaacagttcc tgctcttttt gcggaaattc gccggtggtt
     6361 tcgttaatga agatccccga gtaggcagcc tgacggctgc ggcttgtcat ggtctgcaat
     6421 taccgttata aaaacaggca atgtcattgt ctttcaggtg gttatatggc ccgttcagct
     6481 aaaccccgta aacgtaaacc cgcctctcaa agaagcaaac ttccccgcta tgtcgtgaag
     6541 cttcacgaag atgatttttt tgacgaagaa gacgcagaag ttctgcgctt tgatagtttt
     6601 gacgatgccg ttgaatgctg cgcagacctg aatattccgt tctttgtgga tgcgggaaac
     6661 aaaaagctgg tcttctggtt tgtacgtgtt gatgacgaag ggtatccgga aatcgcccgc
     6721 tgcacggagc gggaatttgc gaccattctt gccggtatca gcgccggcgg tatgtactgc
     6781 ccggagtgtg gcacggttca ctggccggac ggagtcgccc cgcccttctg atgctccccc
     6841 gttttgccga tatttttcag cagggaaacc gctggcttaa ctggctggag aaacaaccgg
     6901 aaggggcagt ccgtccggtg gtaattgaat cagtgacaaa aatcatggcc tgcgggacca
     6961 cactgatggg ctacacgcag tggtgttgtt catcaccgga ctgttgccat acaaaaaagg
     7021 tctgcttccg gtgtaaaagc cgctcctgcc cgcactgcgg ggtgaaggct ggcgcacagt
     7081 ggatacagta tctgctgagc ctggtccccg actgcccgtg gcagcatatt gtgttcacac
     7141 ttccctgcca gtactggtcc ctggtgttcc acaaccggtg gttactggca gagatgagcc
     7201 gcattgcagc ggatgtgata ctggaaatct gccatcaggc agatgtggag ccggggacat
     7261 tcacggtgat ccacacatgg gggcgtgacc agcagtggca tccgcatatc catttatcga
     7321 caactgccgg tggtgtgacg tcgggccaca cctggaaaaa tcttcatttt tacgcccgta
     7381 aggtgatgag catgtggcgc taccggataa cacggttgct gtcacggaaa taccctgacc
     7441 tggtgatgcc ggatgcgctg gcagcagaag gaagcagtaa acgggaatgg aatcgcttcc
     7501 tggacacgca ttaccgccgt agctggaatg tcaacgtatc ccgggtgatg gataatgcca
     7561 cccatgtggc ggtgtgcttt ggctcttacc tgaaaaagcc accggtgccg atgagtcgtc
     7621 tggagcatta tgccggtcag gatgaaatcg gtctgcgtta caacagtcac cgaacaaaac
     7681 gggaagaata cctgttgatg agtggagatg agtccatgga aaggttctcc tggcatgtgg
     7741 cggataaggg gttccgtatg gtgaggtact acgttttttt aagtccggcg aaacggcggt
     7801 tactggaaga agtggtgtac atcataacgg agacagtgag aaaaacggcg atgcaaatca
     7861 cctggagagg gatgtatcag aggttactga aggttgaccc gctgaagtgc gtgctgtgcg
     7921 ggagtcagat gcgttttacg gggctgaagc ggggctaccg tctggcagag caggtcctga
     7981 tgcatgagcc gctggcccgg atgcggtggt gcggctgaga gccgcagagg ggaagttgcg
     8041 tccattttga gggaaacgga gcaaaaaacc gccattcata ccctgaatca atcagagcca
     8101 tccagtttaa tcgtcggttc cgttcatatg gagcaaaagt ggtgttgaag aaacatccgt
     8161 tttgtggtgt ttttttaatc tttttggggt tttaattcct atcgatgaac gttcgctgag
     8221 tgtgaatacc gctgccgctg gcggtaacgc cagtttaatg acgatcggtt aaacaggtag
     8281 ccggaggatg ttacaacctc ctccgtcatc tttaacgtgg tcgaatcccc tcaataataa
     8341 tccgctgcac gttttcaacc gtttgattga aaaatacctc atcgcgcaac gtcgcgcctg
     8401 tcaccgcctc cacctgaggg gcgaaatcgg cgtaatgttg agtggaagcc caaatcataa
     8461 aaatcaaatg ctgcggatca atcggcgcga gtttgccgct tttgacccaa ccggcaatca
     8521 gcgccgattt ctcatcaatt aatgccttca aatcgcccgt cagttcatcc attaacaacg
     8581 gcgcgcctgc cagcatctcc atacagaaca ggcgcgaagc ctgcggataa tcgcgtgaga
     8641 cttccagctt cagacggatg tactctttta tcgccgccag cggggcgaaa tcttcacgaa
     8701 acgcttttaa cggtgccagc cagatatcga gaatctgccg cagcacggca atatacagcg
     8761 cctcttttga cgggaagtaa tacagcagat tggtttttga aacacccgcc aactctgcgg
     8821 tctgctccag tcttgtgccg tgaaaaccga attgtgaaaa agtgtccagt gctgcgctaa
     8881 gaatcgcttt tttcttcgcg cttactgcgc gcgaacgttt acccgttgtt ttcactgcgc
     8941 cttgcgtcat gcgctctccc ctcttgtaga ttggtaacag gatagcaaaa cccccgcccg
     9001 gcctcgacct tctgcactct catcgcgcac ggatgcatgt tttatgtgca actgttttga
     9061 ccgtttagtc cactttttac cagatatttt agataacaca atgctaacac attaataaca
     9121 ttcacttttt aaaaactggc atccgctttg caaacaagcc aatacgcggc tgaaaaggat
     9181 atgcaggatg cagcgccccg tctgactttc actttacgag acgaagagag gttgatgatg
     9241 aaaattggcg tattcgtacc tattggcaac aacggctggc tcatttcgac ccacgcgccg
     9301 cagtacatgc cgacctttga actgaataaa gccattgcgc aaaaagcgga gcattaccat
     9361 ttcgatttcg ccctgtcgat gatcaaactg cgtggctttg gcggcaaaac tgagttctgg
     9421 gatcacaacc ttgagtcgtt caccttgatg gcgggtctgg cagccgtgac ctcgcgcatt
     9481 cagatttacg ccactgctgc caccttaacg ttacctccgg caatcgtcgc ccgtatggcc
     9541 gcaaccatcg actccatctc tggcgggcgt tttggcgtca acctcgtgac tggctggcaa
     9601 aagcccgagt atgagcagat gggtatctgg cctggcgatg actatttctc ccgtcgttac
     9661 gactatctca ccgaatatgt tcaggtgctg cgcgacctgt ggggcacggg gaaaagcgat
     9721 tttaaaggcg attttttcac catgaatgat tgtcgcgtca gtccgcaacc gagtatcccc
     9781 atgaaagtga tctgcgccgg gcaaagcgac gctggcatgg cgttctccgc ccagtatgcc
     9841 gatttcaact tctgtttcgg caaaggcgta aatacaccca cggctttcgc cccgaccgct
     9901 gcgcggatga aacaggccac agagcaaacc ggacgcgacg ttggctctta tgtgttgttt
     9961 atggtgattg ccgatgaaac cgacgatgcc actcgcgcca aatgggaaca ctacaaagcg
    10021 ggcgcggatg aagaggcgtt aagctggcta accgaacaaa gtcagaaaga tacccgctcc
    10081 ggtactgaca ccaacgttcg tcagatggcc gatcccactt cggcggtaaa catcaatatg
    10141 gggacgttag tcggttctta cgccagtgtc gcgcgcatgt tagatgaagt cgcaagcgtg
    10201 cctggtgcag aaggcgtgct gttgaccttc gatgattttc tgtcgggaat cgaaaccttc
    10261 ggcgagcgca ttcaaccact gatgcagtgc cgcgcccatc tctctgcgct gactcaggag
    10321 gtggcatgat gacaacctta accgctcgac cggaagccat taccttcgat ccgcagcaaa
    10381 gtgcgcagat cgtggtggat atgcaaaacg cttatgccac gccaggcggc tacttagatc
    10441 tcgccgggtt tgatgtctca accactcgcc cggtcattgc caacattcaa accgctgtga
    10501 ccgcagcgcg agcggcaggg atgttgatca tctggtttca aaatggctgg gatgaacagt
    10561 atgtcgaggc tggcggcccc ggctcaccga attttcataa atcgaacgcc ctgaaaacca
    10621 tgcgtaagca gccgcagctg caggggaaat tgctggcgaa aggctcctgg gattatcaac
    10681 tggtggatga actggtgccg cagcctggcg atattgtgct gccgaagccg cgctacagcg
    10741 gtttcttcaa tacgccgctg gacagcattt tacgcagccg tggaatacgc catctggttt
    10801 tcaccggtat cgctaccaac gtctgcgtcg aatcgacgct acgcgatggc ttttttctgg
    10861 agcatttcgg cgtggtgctg gaagacgcaa cacaccaggc ggggccggaa tttgcacaga
    10921 aagccgcgtt gttcaatatc gaaacctttt ttggctgggt cagcgacgtc gaaacgttct
    10981 gcgacgcgct ttctcccacg tcctttgctc gtatcgctta aggagtttag cgatgccaaa
    11041 atccgtaatt attcccgctg gcagcagcgc accgctggcc cccttcgttc ccggcacgct
    11101 ggctgatggc gtggtgtatg tctccggtac gctggctttt gatcaacata ataacgtgct
    11161 gtttgccgat gacccaaagg cgcaaacccg ccacgttctg gaaactatcc gcaaggtgat
    11221 cgagacggcg ggtggcacga tggcggatgt gaccttcaac agcatcttta ttaccgactg
    11281 gaaaaattac gccgcgatta acgaaatcta cgccgagttt tttccgggtg ataaaccggc
    11341 gcgattctac attcagtgcg gactggtaaa acctgacgcg ctggtggaaa tcgccacaat
    11401 tgcgcatatc gccaagtgag gccgcgatga aactttcact ctcacctccc ccttatgctg
    11461 atgcacccgt agtggtgttg atttcgggtc ttggcggtag cggcagttac tggttaccgc
    11521 aactggcggt gctggagcag gagtatcagg tagtctgtta cgaccagcgc ggcaccggca
    11581 ataatcccga cacactggca gaagattaca gtatcgccca gatggcagcg gaactgcatc
    11641 aggcgctggt agccgcaggg attgagcgtt acgcggtggt cggccatgcg ctcggtgcgc
    11701 tggtggggat gcagctggcg ctggattatc ccgcgtcggt aactatgctg gtcagcgtta
    11761 acggctggct acgaataaac gcccatacgc gccgctgttt tcaggttcgc gaacgattac
    11821 tgtatagcgg cggcgcgcaa gcatgggtgg aagcgcagcc gttgttcctc tatcccgccg
    11881 actggatggc ggcccgcgca cctcgcctgg aggcagaaga cgcgctggca ctggcgcatt
    11941 ttcagggcaa aaataattta ctacgtcgac ttaacgccct caaacgcgct gactttagtc
    12001 accatgcgga tcgcatccgc tgcccggtgc aaatcatctg cgccagtgat gatctgctgg
    12061 tgccaacagc atgttccagt gaacttcatg ccgccctgcc cgatagccag aaaatggtga
    12121 tgccctatgg cggacacgcc tgcaacgtga ccgatcccga aacgtttaat gctctgttac
    12181 tcaacgggct tgccagcctg ttacatcacc gtgaagccgc cctgtaagga attgctatga
    12241 acgaagccgt tagcccaggt gcgcttagca ccctgttcac cgatgcccgc actcacaacg
    12301 gctggcggga gacacccgtc agcgatgaga cgttacagga gctttatgcc ctgatgaaat
    12361 gggggccgac atcagctaac tgttctccgg cacggatcgt gtttatccgc actgcagaag
    12421 gaaaagaacg tctgcgcccg gcgctttcca gcggcaatct gcaaaaaacc ctgaccgcgc
    12481 ccgtcaccgc tatcgtcgcc tgggacagtg aattttatga acggttacca ctactgtttc
    12541 cccacggtga tgcccgcagt tggtttacct ccagcccaca acttgccgaa gaaacagcgt
    12601 ttcgcaacag ttccatgcag gcggcttatc tgatcgtcgc ttgccgtgcg ctgggactgg
    12661 ataccggccc gatgtcgggt tttgaccgtc aacacgttga cgacgccttt tttgcgggca
    12721 gcacgctgaa gagcaatctg ctgattaata tcggctatgg cgatagcagc aaactttttg
    12781 cgcgcctgcc acgtctgtcc tttgaagaag cctgcgggct gttgtaagga gcaaactatg
    12841 aacatagttg atcaacaaac ctttcgcgat gcgatgtcct gtatgggcgc ggcggtcaat
    12901 atcatcacca cggacggtcc agccgggcgc gccgggttca ccgccagcgc cgtctgcagc
    12961 gtgaccgaca cgccgcccac attactggtg tgcctgaatc gtggggcgtc cgtctggccg
    13021 gtattcaatg aaaatcgaac gctgtgtgta aatacactga gcgccgggca ggagccgctt
    13081 tcaaatcttt ttggcggcaa aacgcccatg gaacaccgct ttgccgccgc ccgctggcag
    13141 acgggtgtga ccggatgccc gcaactggaa gaggctctgg tttcgtttga ctgccgtatc
    13201 agccaggtgg tgagcgtcgg cacccacgac attctgtttt gcgccatcga agcgattcat
    13261 cgtcacacca caccctacgg gctggtgtgg ttcgatcgca gttatcacgc gctgatgcgc
    13321 cctgcttgtt aacctcctta aggagacagc tgatggcaat gttcggtttt cctcactggc
    13381 agttaaaatc gacctctaca gagagcggcg tggtcgcgcc ggatgaacga ttgccgtttg
    13441 ctcagacggc agtaatgggc gttcaacacg cggtggcgat gtttggcgcg acggtgttaa
    13501 tgccgattct gatggggctg gatcccaatc tttccatttt aatgtcgggg atcggcacgt
    13561 tactgttttt ctttattacc ggcgggcgcg tccccagtta tctcggctcc agcgcggctt
    13621 ttgtcggcgt ggtgattgcc gcgacgggct ttaacggtca gggcattaac ccgaatatca
    13681 gcatcgcgct gggcgggatt atcgcctgtg ggctggttta taccgtcatt ggcctggtgg
    13741 tgatgaaaat tggtacacgc tggattgaac gactaatgcc gccggtggtg acgggtgcgg
    13801 tggtgatggc gattggcctg aacttagcgc ctatcgcagt gaaaagtgta tcggcttcgg
    13861 cctttgatag ctggatggcg gtaatgacgg tgctgtgtat tgggctggtg gcggtgttta
    13921 cccgtggcat gatccagcgg ttattgattc tcgtaggatt aattgtcgcc tgtctgctgt
    13981 atggcgtgat gaccaacgtt ttagggctgg gaaaagccgt cgattttact ctcgtcagct
    14041 atgctgcctg gttcggtctg ccgcattttt ccactcctgg ttttaatggc caagcgatga
    14101 tgttgattgc gcccgtggcg gtgattctgg tggcggaaaa cttaggtcat ctcaaggcgg
    14161 tcgccgggat gaccggacgc aatatggacc cgtatatggg gcgagcattc gtcggtgatg
    14221 gtctggcaac gatgctttcc ggctctgtcg gcggtagcgg cgtcaccacc tatgctgaaa
    14281 atatcggtgt gatggccgtg acaaaagtct actcgacgct ggtgtttgtt gcagctgccg
    14341 tcatcgctat gctgttgggc ttttcgccga aatttggcgc gctgatccat accattccgg
    14401 ccgcggtgat tggtggtgca tcaattgttg tgttcgggct gattgccgtc gcaggcgcaa
    14461 ggatatgggt acaaaaccgt gtcgatttaa gccagaacgc taatttgatt atggtcgcag
    14521 taaccttcgt gctgggcgcg ggtgattttg ctctcacgct tggcggtttt acgttgggag
    14581 ggatcggtac agcaaccttt ggcgcgattt tactcaatgc gttattaagc agaaggttgg
    14641 ttgacgttcc accgccagaa gttgttcatc aggagccata aacagaaaac cgccgggtta
    14701 ccggcggtga tgactttttg acgagcttaa gaaaaaaatg cattcgtcat tgattagccc
    14761 ggctagttgt cgcttttgcc gtgactgctc ttgccacctt ttttacctgc ttctgatgct
    14821 ctttcgcggt cttctgcaaa attgccggaa ccgcctcgat ggtttgccat tactgacctc
    14881 cgtctgggta tagacatcat attgcataga attaacgcgg cgtatggcgg ctgccatatt
    14941 ttcgctacgt gggactaagc ttagtgaact gacgcggaca gacccgcgtt tggtaatatc
    15001 ctgcaacagc ttcttagcga gaatagatat catctccaat aattatccat aagccgctct
    15061 tatgattaaa aaaacgcgat gagataaaaa tgtgtcattt tgcgacaaaa ttacgtgctt
    15121 gtgaaagttg ttataaatca aataagtggt tgtgaaattt gcactctgaa aaggacgtct
    15181 tatctttaaa taagtggtag cgaatcgcta cggaatagag ataacacgag gagtggttag
    15241 aaatggctaa agttctggtg ctttattatt ccatgtacgg acatattgaa acgatggcac
    15301 gcgcagtcgc tgagggtgca agcaaagtgg atggcgcaga agttgtcgtt aagcgtgtac
    15361 cggaaaccat gccgccgcaa ttatttgaaa aagcaggcgg taaaacgcaa actgcaccgg
    15421 ttgcaacccc gcaagaactg gccgattacg acgccattat ttttggtaca cctacccgct
    15481 ttggcaacat gtccggtcaa atgcgtacct tcctcgacca gacgggcggc ctgtgggctt
    15541 ccggcgcact atacggaaaa ctggcgagcg tctttagttc caccggtact ggcggcggtc
    15601 aggaacaaac catcacatcc acctggacga cccttgcgca tcacggcatg gtgattgtcc
    15661 ccattggcta cgcagcgcag gaattatttg acgtttcaca ggttcgcggc ggtacgccgt
    15721 acggcgcaac caccatcgca ggcggtgacg gctcacgcca gccaagccag gaagaactgt
    15781 ctattgctcg ttatcaaggg gaatatgtcg caggtctggc agttaaactt aacggctaat
    15841 cttcaacagg aggatacgca tgccaactca agaagcgaaa gctcatcacg tcggtgaatg
    15901 ggcatctctg cgcaatacgt cgccggaaat agccgaggcc attttcgaag tcgccgggta
    15961 cgacgaaaaa atggcagaaa agatttggga agaaggtagt gatgaagtgt tagtcaaagc
    16021 ctttgctaag accgataaag attcgctttt ttggggcgaa cagaccatcg aacgtaaaaa
    16081 cgtttaacca gcgactcccc cgcttctcgc gggggagttt tctgttattt caccgcctca
    16141 ttcaacacgc tatcaaactt atccatcggg cagaaaccgt tggcatctat cgggcaaccg
    16201 cttaattcca gcgtcacgcg ctgcgcaggt gcctgcaggg ttaacgcatc ggcattacgt
    16261 aactgttccg cactctggta cacatattca attttcatca aatcgcgatt ggctttgctg
    16321 tcatgccaac gctggaaaac gattttgccg ccaatcggcg tgcgttcgtt ctggtcatgc
    16381 aactgatacg gtttgaaatc cagcgccgtt aacagagagg ctatgttgga gtcgtgccca
    16441 actaacaccg tgattttcgg agcactggcg cgatcggtga ccagggcttt gtcgatataa
    16501 ctgaccagcg gtttcgcaac attgcgcgcc acttccggtg aggtaaacag actgtcctgg
    16561 taaccgtttt tcagcttcga caataccttc cactgctggt cagatttgat ttctccccag
    16621 gccacctgat ccatcggaaa accttcgtag tattgcaaag taaacgcatc taccagcgag
    16681 ttgccgactt tcagcggccc ggaaacacct ggttcttgtt gatacttggc gctaaaggta
    16741 tttttgccat ccaccagcga acactgttgt ttctctttac aggcagggga atctttatag
    16801 ttaacgattt tttccagtag ctggtagctg tcggtaagct ggagtttgct gagctctttc
    16861 tccattgccg ccaccgcctg ttcactgaat gcggcggaat catcggtgat catcgggtta
    16921 aaggttgggt ccatagtgcc cattttttcc tggtgatgca caggaatatc acacccaggg
    16981 aatgcaccag taataaagaa ctgggcggta gcgacggtac gttgcagact attggcatag
    17041 gcataaacgg tgtccggcgg cgggcattcc cccgatttca ctatcccctg ctctgccagc
    17101 cattcacgca tgtaatggcc catatacact tcgagcacgc cacctttggt ggtgagttgc
    17161 ccaccgggga cgtcccattc gggccattta ttcggcgtcg actgctccag cacactgcca
    17221 ttgttcgcca gcggcgcacg taagttatgg cggctcatca tgagcacttg ctgtagctga
    17281 tagccttccg gtacggtttg tgcctgagca tttgaagcga gtaaaactat ccctgccaca
    17341 gttgcggcga ttagcgtttt gttcattcct ggcacctctt ttgttatcaa tcattaaaga
    17401 gtgtgacaga aatatgactt ttttccggga acaggttcgc aaaatggctt gttgaacgcc
    17461 tgtttttcta tatacatctt caaaaacagg caaggtttga cgcaaactat gcctgattag
    17521 cccctgcctc gcgtacgccg ctgataattg cacctgtggc taaacagaat atctgcggcg
    17581 ttaccaatac agagacaata tttaccaata aaaaagtgtg agactaataa caatggtttt
    17641 tagcatatgc ctttgccatt aagttgcata acgatttaaa tagtcaagtt gcgcattcat
    17701 ttccttattt tttaacttat aagcctgaga aaaaagttca ggagggaggt aaacatcata
    17761 atgattgcca gtgtttgtta aataaccata ctcaagatct tcatctgttg aaaaggattc
    17821 atttcccaag gagattgatg ccacattact attaacaata aataatgagc aaatgctatc
    17881 tgaaagaaca gcaagaaaat tcttcaccgc tttaatccta tccgacgtgt cggtatctaa
    17941 agcaagcaaa ttcttgttat cattaaatat aaactcgggt aaatgcagta tgcatttttt
    18001 gtcattcaag caactatcaa tgaattgttt tttaaaaaac agaaagctcc atgacataaa
    18061 actacccaca ccctgaattt tgttgtcaga ttgcagatca acgccaaata atgtctttac
    18121 atcttccctg agattataga atgccgtatc aacaagagcc tcgatcgttt gtttatcaac
    18181 aattttaata aactcttcgt atacttgctt ttgcgtctta tggctgccca gcgcccatga
    18241 atactcatgt tcttttaaaa ccagtatggc tcttaacaga caatatccat cgccatctac
    18301 tggagcgtat tttaatccat atgttttatt aatttcactc tgaataaaag atatgccaga
    18361 caaatcaata ccagtatcta aatcagatat tcccggcata tttatctgtt ctggctggat
    18421 actcatattt tcatcatcta aacaatactc agaaaaaaca tcatcacacg cattaatgtt
    18481 ttcaatatat tccataaagc cataccaata ataaccttta aaatctgtga taggctctga
    18541 acctctgccc ggcaaggaca ctctaacaat atcaacatct tgaacatatt gtatagtata
    18601 tgtaatacct cctgaaaccc ctgaataaat ttgtgtatca ttagtacgca atgttctcat
    18661 aaataatgat agatcattat cattctgcat ttcctcaaat tctttttcta gtgattcaca
    18721 ttccagacat ggagtatcct cttcatttac tttacgtctg agttcattgt acaaccgcat
    18781 aaaacacacc agtgtgtttt caaaaagcgt gattgaatat gtttgatttt tttcgtactc
    18841 attttttata tcgttccagg cctgtttcaa ataattattt gcagtgcaac taattccatg
    18901 tatattacta cccatatata gcgtctataa aatttaataa ataatgacgc cctagttaaa
    18961 cttaaagtgc ctggttcaac tatcaaaaat cgctcaccct ttttcacctg tttaaaatat
    19021 gttcagcaac ccatcttgat ggcgacctcc tctccgcgat gatttcaata acatattctg
    19081 tgttggcata tgaaattttg aggattaccc tacacttata ggagttacct tacaggggtt
    19141 ccttcaattt gtgttgattt acgcgagata acgctatgga attaaaggat tattacgcca
    19201 tcatgggcgt gaaaccgacg gacgatctca agacaatcaa gaccgcctat cgtcgactgg
    19261 cccgtaaata ccatcctgat gtcagcaaag aaccggatgc cgaagcccgc ttcaaagagg
    19321 tcgctgaagc ctgggaagtg ttaagtgatg aacaacgtcg cgctgagtat gatcagatgt
    19381 ggcaacatcg caacgatccg caatttaacc gtcagttcca tcatggcgac ggtcagagtt
    19441 ttaatgccga agattttgac gatatcttct cgtcaatttt cggtcagcat gcccgccaga
    19501 gccgtcaacg ccccgccgca cgcggccacg atattgaaat cgaagtggcg gtattcctcg
    19561 aagaaacgct tactgagcat aagcgtacca tcagctataa cctgccggtt tataacgcct
    19621 ttggcatgat cgaacaggaa atcccgaaaa cgctgaatgt gaagatcccg gcaggcgtcg
    19681 gcaatggtca acgcatccgc ctgaaaggcc aggggacgcc gggcgaaaac ggcggtccaa
    19741 atggcgattt gtggctggtg attcatattg cgccacatcc gctgttcgat attgtcggcc
    19801 aggatctgga aattgtggtg ccggttagcc cgtgggaagc ggcgctgggt actaaagtca
    19861 ccgttccaac actgaaagaa agcattttgc tgactatccc gccaggcagc caggccgggc
    19921 aacgattgcg cgttaaaggc aaaggtctgg tgagcaaaaa acagaccggc gatctgtatg
    19981 cggtactgaa aatcgtgatg ccgccgaaac cggatgaaaa cactgccgcg ctgtggcagc
    20041 aactggcaga cgcccagtcg tcttttgatc cacgtaaaga ttgggggaaa gcataatggc
    20101 taatgttacg gtgactttta ctattaccga attttgcctg cataccggca tctctgaaga
    20161 ggagttgaat gaaattgtcg gtttgggggt ggttgaaccg cgtgagatcc aggaaacaac
    20221 ctgggtattt gacgaccatg ccgccattgt ggtgcaacgc gcggtacgcc tgcgtcatga
    20281 actggctctg gactggccgg ggatcgcggt ggcgctgacg ttaatggatg atattgcgca
    20341 cctgaagcag gaaaaccgcc tgctacgcca gcggctttcc cggtttgtag ctcatccgtg
    20401 atgtgtgttt gccggatgtg atgacgcatc cggcaactgc gccataatgc ctgatgcgac
    20461 gctaaacgcg tcttttcagg cgcataaacg acgttatctg ttttggtggt cgcactccac
    20521 taacaccagc aataactggc ttagtgccgc gtaaaaacca aagctgtcat actgtcgaca
    20581 acgcgcagca aactctggta accattgccg cagcgccgtc agtgtttttt gccgcaaact
    20641 gtcgattctt cgcgcaggaa cggtcccctc tcccagcgaa aaatgcagat aactcaacaa
    20701 ctcgagatag atcgccagat gatctgccgg ttcgttgaaa ttgccactgg tttccatccc
    20761 tgcctcaact aacaagcgtt taatctcttg ctcgtcctgt ttgtaggccg atgcatacgg
    20821 cagcgccgct tgtttgtcag tcatcagaaa caggccgcaa aagtcagcgg ccagttccag
    20881 acgggcatcg tcacgtactg tcagcgt
//
LOCUS       DAEEUE010000068        20837 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.068, whole genome shotgun sequence.
ACCESSION   DAEEUE010000068 DAEEUE010000000
VERSION     DAEEUE010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20837
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.068"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="KLK84_002692"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="KLK84_002692"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HBE2009960.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="KLK84_002693"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="KLK84_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HBE2009961.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /locus_tag="KLK84_002694"
     CDS             complement(2649..3200)
                     /locus_tag="KLK84_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HBE2009962.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="KLK84_002695"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="KLK84_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HBE2009963.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5215
                     /gene="cybC"
                     /locus_tag="KLK84_002696"
     CDS             4829..5215
                     /gene="cybC"
                     /locus_tag="KLK84_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HBE2009964.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="KLK84_002697"
     CDS             complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="KLK84_002697"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HBE2009965.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="KLK84_002698"
     CDS             complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="KLK84_002698"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBE2009966.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8414..10069)
                     /gene="treC"
                     /locus_tag="KLK84_002699"
     CDS             complement(8414..10069)
                     /gene="treC"
                     /locus_tag="KLK84_002699"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBE2009967.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10119..11540)
                     /gene="treB"
                     /locus_tag="KLK84_002700"
     CDS             complement(10119..11540)
                     /gene="treB"
                     /locus_tag="KLK84_002700"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HBE2009968.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11659..12606)
                     /gene="treR"
                     /locus_tag="KLK84_002701"
     CDS             complement(11659..12606)
                     /gene="treR"
                     /locus_tag="KLK84_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HBE2009969.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12791..12844
                     /gene="mgtL"
                     /locus_tag="KLK84_002702"
     CDS             12791..12844
                     /gene="mgtL"
                     /locus_tag="KLK84_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HBE2009970.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12985..15681
                     /gene="mgtA"
                     /locus_tag="KLK84_002703"
     CDS             12985..15681
                     /gene="mgtA"
                     /locus_tag="KLK84_002703"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBE2009971.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="KLK84_002704"
     CDS             complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="KLK84_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HBE2009972.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="KLK84_002705"
     CDS             complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="KLK84_002705"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HBE2009973.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="KLK84_002706"
     CDS             complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="KLK84_002706"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBE2009974.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="KLK84_002707"
     CDS             complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="KLK84_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HBE2009975.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18174..18569)
                     /locus_tag="KLK84_002708"
     CDS             complement(18174..18569)
                     /locus_tag="KLK84_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBE2009976.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="KLK84_002709"
     CDS             complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="KLK84_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBE2009977.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19485..20078
                     /locus_tag="KLK84_002710"
     CDS             19485..20078
                     /locus_tag="KLK84_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBE2009978.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20223..20675
                     /gene="tabA"
                     /locus_tag="KLK84_002711"
     CDS             20223..20675
                     /gene="tabA"
                     /locus_tag="KLK84_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HBE2009979.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
BASE COUNT         4963 a         5226 c         5520 g         5128 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgctg gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgccgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaagtaaaag aagcgcaggc
     5161 tgctgcagag caactgaaaa cgacccgcaa cgcctatcac cagaagtatc gttaattcct
     5221 catttccctg ttgcctgcac tcaggtaaca gggaacttat catcgcaaat gatgcaccac
     5281 ctgattgctg ctgccgcgcc agatcaggga agggtctttt aaatcctgca caaatttgcc
     5341 gtcgaccagc acgttaatca aatcaacaac ctgcatttgc gcagcgttga gttcgtcgag
     5401 tttatagcct gtccacaccc agatgtcttt acccggacac tcggcgcgga tgcgttttac
     5461 cagtttcaga atatccggca cgttttgcgg atgcagcgga tcgccgccgg agagggaaat
     5521 cccctggcgt ttgatacgag tgtcattcag atctttaatg atcttgtctt ccattgcttt
     5581 ggtaaatggc tgaccagaat ttacccgcca tgtgcttttg ttatagcaac cggggcattc
     5641 atgaacacac ccggagacaa acagggtgca gcgagtgccg gggccgttga cgatgtcgac
     5701 aggatagtac tgatgataat tcattatttc gcctggctta tcagtccgcg tgtaggctgc
     5761 atccggtaat caatgcctga tgcgacgctg tcgcgtctta tcaggcctac agccgttgcc
     5821 aaatgtaggc cggataaggc gtttacgccg catccggcat ttgcttagcg cagagtgaag
     5881 attaacctat ctgcccattc cccaaatgtt taacgcggcg cttaacttct tcctgcttac
     5941 cagcgttaaa cggacgtgca tccgggctac ctaaatatcc gcacacgcgg cgagttaccg
     6001 acacacggga ggcgtcatgg ttaccacatt tcgggcaagt gaagcctttg ctggtgcact
     6061 cgaactcacc ggtaaagcca cactcgtagc actcatcaat cggtgtattg gtgccgtaat
     6121 acggtacatg ctgatagctg taatcccaga catcttccag cgctttcagg ttgtgctgaa
     6181 tgtttggata ctcgccgtag caaatgaaac caccgttcgc cagcggcggg taaggcgctt
     6241 caaagtcgat cttgtcgtac gggttcacct tcttctccac atcaaggtgg aaactgttgg
     6301 tgtagtaacc tttatcggtc acgcccggca ccacgccaaa ctcagcagta tcgagacggc
     6361 agaagcggtc gcacaggttt tcactcggcg tgctgtaaag actgaaacca taacccgttt
     6421 cttctttcca ctgatccact gcctgacgca gacgttcaac aatggcgata cctttcgcgc
     6481 gaagcttctc gttgtcgtaa acatgctcgc cgctgaacag cgcgttaatg gtttcgtgga
     6541 tgccgatgta acccagcgaa atagacgcac gaccgttttt gaagatttca gaaacatcat
     6601 cgtcagcatt cagacgtacg ccacaagcac cttccatata gaggatcggg gcgacgcgcg
     6661 ctttcacgcc ttcgagacga gcgatacggg tcatcagtgc cttacgtgcc agcaccagac
     6721 gttcatccag cagcttccag aaggtggctt catcgccttt tgcttccaga gcaatacgcg
     6781 gcaggttcag gctgatcacg ccgaggttgt tacgaccatc gtggatctgc tcgccgtttt
     6841 cattttccca cacgccgagg aagctgcggc agcccatcgg ggttttaaac gaaccggtga
     6901 cgttcactac ctgatcgtag ttcaggatat ccggatacat gcgcttgctt gcgcactcca
     6961 gcgccagctg tttgatgtcg tagttcggat cgcctttttt atggttcagg ccatcgcgaa
     7021 tcgcaaacac cagtttcggg aacaccgcag ttttacggtt tttaccgagg cctgcgatac
     7081 ggttacgcag gatcgattcc tgaatcaggc gcgattccca gctggtgccc agaccaaaac
     7141 caaaggttac aaacggcgtc tgaccgttgg cggtgtgcag ggtgtttact tcgtactcca
     7201 gcgactggaa ggcatcgtag cactctttga tggtacgaga gttagcgtag ccttcggcgt
     7261 ccgggatgtt ccactcttct gcggtcttgc gatgtttgtt gtagctggcg gtaacaaacg
     7321 gcgccagcac ttcatcgata cggttaatgg tggtgccgcc ataaatatgg ctggcgacct
     7381 gagcaataat ctgcgcagtt accgcggttg ccgtagagat cgacttcggc ggttcaatct
     7441 cggcgttccc cattttaaaa ccctgggtca gcatgccttt caggtcgatc aacatgcagt
     7501 tgaacatcgg gaagaacggc gagtaatcga gatcgtgata gtgaatatcg ccacgctcat
     7561 gtgcctgcac cacgtcacgc ggcagcaggt gctggcgtgc atagtgttta gccacgatcc
     7621 cggccagcag gtcgcgctgg gttggaatca ccttgctgtc tttgttggcg ttttcgttga
     7681 gtaacgaggc gttggtctgc tcgaccagac cacggatctc ctggttcagg cgaccgcgtt
     7741 tttcacgttc aatgtcgcga tcgtgacggt actcgatgta agcacgagcc agttgtttgt
     7801 atggacccga catcagctgg ttttcaactg cggtctggat ctcgttgata tccacctggt
     7861 tgcgcccctg catctgctcg ttgacaaccg cggcaacagt ggcgcaataa tcggcatcat
     7921 cgacttccgc tgctttagct gcacgcagaa tcgcttcttt gatgcgctct gatttaaacg
     7981 gcactttgca gccgtctcgt ttcatcacat gcggtgtcat gatcgctcca tatttttaag
     8041 aacaggttat ccacagaaat tgggaaaggc gttttccggt tttttcgttt cgttttccgg
     8101 tgctttccgc aatcgccatc cgcttatcca caacgccgca cctttttcgt ggggagctgt
     8161 agtagcaatt atagtcgatt aatacaacat attgggttgg gatgcatttt aaagtctata
     8221 tatagtgctt tgcatcaagg atgttttaac tttttttgat gtagctcaaa gtaaaaagca
     8281 gagcgtacgg atgacgggcg ctacagcgtt atgtaaattt tttaatgaat ttgctggttg
     8341 aaaaatcaac aaaaacaaca tgctgacaga caaaaccccg gaatgaccgg cattatcggg
     8401 gcttagggaa gatttacttc tgtaaccacc agacagcctc aaaaggccgt aaattcatgg
     8461 cacagggttg tggtgaggct tcttcgtaat tatgcatcac aagctgccag ttgccgcgca
     8521 tttgccctgg ctgccagggt tggatctcac ggctaaggtt ggcaatgacc agcaaggttt
     8581 gccccttcca ttcacggcga tagcaccaca atacagggct gtttggcagc agatcctggt
     8641 aattgcccca tgtcaggatg gcttcttgct tacgcagtgc gattaacttt tggtaggtgt
     8701 aaaacacaga ggagtcatcg gccagcgcgg cttctacgtt gatttgttga tagttatcgc
     8761 ctaggccaat ccacggttcg ccagccgtaa acccggcatt atcgccgttg ctccattgca
     8821 tgggcgtgcg gctgttgtca cgggatttac tggcgaggat tgccaataac tcgtcggcat
     8881 cacgaccatc gttgcgcagc tcggcaaaca tattgaggct ctccacgtcg cgatagtcag
     8941 taatgcgcgt gaaatgcggg ttggtcatgc caatctcttc gccctggtag atatacggcg
     9001 ttccctgcat gccatgcagc accatcgcca gcatttttgc cgcaggcacg cggtattcac
     9061 cttcatcacc aaagcgagaa acaatgcgcg gctgatcgtg gttacaccag aacaaggcat
     9121 tccatgccac gttgtgcatt ccttgttgcc agtggcggaa caatgttttc aacgccacaa
     9181 agtcaggttt agccagcgtc catttttcac caccgggata atcgaccttc aggtgatgaa
     9241 aattaaaggt catcgacaat tcactgcctg tcagtgccgc gtatcgctgg caatgctcaa
     9301 ggctggtgga ggacatttca cctacggtca ttaacccgcg tggcgtaaac acatcgcggt
     9361 tcatctcgtg caaaaactcg tgtgctcgtg gcccgtcggt gtagaagcga cgcccgtcgc
     9421 catccaggtc ttcagggaaa cgcgggtctt tggagatcag attcaccaca tccaggcgca
     9481 acccgtcgac cccacgatcg gcccagaatt cacagacttt tttcagctct gcgcgtaccg
     9541 ctggattctc ccagttgaga tccgcctgtt ctggtgcaaa gagatgcaaa tagtactgtt
     9601 cgctttccgc atgccagcgc cacgcactac cgccaaattt tgaacgccag ttgttcggtg
     9661 gcgtttctgg ttcgccatcg cgccagatat aaaactggcg gtaagggctt tctttgttca
     9721 gcgcctcgcg aaaccaggca tgttgggtag aggtatggtt aaacaccata tcgagaatga
     9781 tacgaatccc gcgcgatttt gcctgcgtca ccagttcgtc aaaatcgtcc agcgtgccgt
     9841 aggtgggatc aatcgccgta tagttcgcta catcgtaacc gttatcgacc tggggagaga
     9901 cataaaaggg ggttagccag atggcatcaa cgcccagttt atgcagatag tccaggcgtt
     9961 ggataacgcc acgtaaatcg ccggtgccgc tacccgtggt gtcctgaaaa ctctttggat
    10021 aaatctggta gataacgccg ttttgccacc agtggggaag attagtcatt acgttattcc
    10081 tgcaaatgcg agggggcgca attgcgcccc gaagaaaatt aaacaatgtc cagcgtgccc
    10141 aggcggtatt tccgctgata gataaacgag gtgagtacaa tcgggatgat gatggcgata
    10201 gccattgcca gcgcaaatac ctgccagtag ctcggttgaa tagagagaat tcccggcagg
    10261 ccccctacgc cgataccatt cgccataacg ccgttcaggc cgcatagcaa tcctgccaga
    10321 ccagaaccaa tcatcgcgca cagcatcggg aagcgatatt tcaggttgat gccgtacatt
    10381 gcaggctcag tgaccccaag ccaggcggag atagcggcag gcacggagat ctcgcgttca
    10441 ttgtgcttgc ggctggaaat gataatgcct atcacggcgg agccctgagc gatattcgac
    10501 agcgcaatca gcggccacac tggcgtgcca cccatgcttt gaatcatctg caaatcaata
    10561 gcaagggtgg tctggtgtac gccggtgatc accagcggcg cgtacaggaa gccaaacaat
    10621 gccgcaccaa tcggagcaaa gctgccggtc atcaggtgac gtaccgcaaa ggcaacgcca
    10681 tcgccaatca tgcgaccaaa cggaccaatc agcgcatggg cgaggaacac cgcgaggatc
    10741 agcgaacaga cgggcaccac caccagatag aggtaatccg gtacgatgcg tttaaggcga
    10801 gtttcaataa cgcccagcgc cagcccggct aacagtgccg ggatcacctg cgcctgatag
    10861 cccactttgg cgatgctgaa catgccaaag tcccacactt ccggcagctg ctgcccgagc
    10921 agataagcgt tcatcagctg gggggaaacc agtgtcacgc caagcacgat accaaggatc
    10981 ggcgtgccgc ccattttttt caccgctgac cagcaaatcc cgaccggcag gtagaagaaa
    11041 atcgcttcac cgatcaacca cagaaaatcg tagatcgttt gcagggaagg gtacatttgc
    11101 gccagcgtct gaccgttgct catgggcaaa tcgccgatca cattgcgaaa accgaggatc
    11161 aaaccgccgc taatcaacgc gggcagcaac gggaagaaga tctccgcgaa atgagagatc
    11221 aactgctcat gccatttcat attttgccgg gcggcttttt ttacctgctc tttatcaacc
    11281 tgcgcctgtc cggttgacgc tattagtgct tgatagtaat cacccacgtt ggtgccaatc
    11341 accacctgaa attgcccggc attggtgaaa cagcctttca ccatggggag ttgctcaatt
    11401 tctttcggtc tggcattggc cggttggttg aggacaaagc gcaggcgagt aatacagtgg
    11461 ctcaccgtcg caatattgcc gcgcccgccg accagttcaa tcaaccgatc gatatccgtt
    11521 tggtttattt tgctcatcat aaagccccat ggcagatgac atttttggtt ggctgcagaa
    11581 tatattgcgc ggaaaaattt aaaaacggga acgttcccga aacgcagcga agatcacaat
    11641 ttatcgttca ggaaacgatc aggacagggt ggcggggatg atgatttgtt gcggttcgct
    11701 gcgcccggtt acctgtgcga tcaattggca ggccgcctgg cgtccagctt cggcgtagcc
    11761 aggatctacg gtgacgatct ccggatgaag gaatttcatt aacggcgtat tacctacgct
    11821 cgccagttgc aaggtgtcga tgcgttgctc ttgcaggtat ttacttgcgc caagagccag
    11881 cgtgtcggtt gcgcacagta acgcggtagt ttcaggcgta atcacttttg ctacgttttc
    11941 atagccttgc ttcatagcaa gccctggcag agcggcaacg ggatgcagtt tatgcgcttt
    12001 gcagaacgcc aggtaggctt cgtgacgtcg cttcccggtc gtcacgtcac tatgcggcac
    12061 gccgagataa ctgatattgc gatgcccctg gtcatagagc cgttgcatca gaatcttgat
    12121 tgccccttcg tcgtcataac agaccgaagc aaagcctttt gcgtcacgcg ccagcagaac
    12181 cagcgatgac tgccagtggg ctaacatttc ttcggttatg ccggtaaaac cgaacagcac
    12241 tacgccgtcg atattacgcc gtttcagcac ccccaaatgt tcggcaacta attgtgggga
    12301 aaactgattt tccatcatga ttggatcgta accttgttca tagaacgctg gcagcatggt
    12361 ttgaacggcg agattttctg acaacgaatc cagacgggta acaatgatgg cgaccacttt
    12421 atcgctttgc ccacgcatag cgcgcgcaga gcgggaaggg gaaaatccat gctgattcat
    12481 cactgcttca acacgctcgc gcgtgcgctg actcacgccg ctttcgttat tcagcacccg
    12541 ggaaaccgta gatttcccca cgccgcttaa gcgcgcgatg tctttgatgg tcagccgatt
    12601 ttgcatcctg ttgtcccata acgtgttgtt taattatttg agcctaactt tacccgtgca
    12661 ttcagcaatg ggtaaagtct ggtttatcgt tggtttagtt gccagcaggt attatatcgc
    12721 cataaatgct acgaatatta ttggattctc cttattattt gcggcgcttt tttcacttac
    12781 cggaggttat atggaacctg atcccacgcc tctccctcga cggagattaa aacttttccg
    12841 gtaagcccgt cttttcacgg cgttaccgga tgcgtaaggc cgtgacgttt taatgtccct
    12901 gctcagcttt attaccttca ggtaaggctt cgccacgcct gaagacattt ctgtactgtt
    12961 tcagacagtg cggagggact ccttatgttt aaagaaattt ttacccggtt cattcgccat
    13021 ttaccttccc gtctggttca tcgcgatcca ttgcctggcg cgcagcagac agtgaatacg
    13081 gcggtcccgc cgtccttaag tgcgcattgc ctgaaaatgg cggtgatgcc cgaagaagaa
    13141 ttgtggaaaa cgttcgacac ccatccggaa ggattaaatc aggcggaagt ggaatctgcc
    13201 cgcgaacaac atggtgataa taaattaccc gcccaacaac cgtcgccgtg gtgggtacat
    13261 ttatgggtct gctatcgcaa cccctttaat attttgctca ccattcttgg tgctatttct
    13321 tacgccacgg aagatttatt tgccgcaggt gttatcgcgc taatggtcgc tatttctacg
    13381 ttgctgaact ttattcagga agctcgttcc accaaagcgg cagatgccct gaaagcgatg
    13441 gtcagtaata ccgcgacggt actgcgagta attaacgaca aaggcgaaaa tggctggctg
    13501 gagatcccga tagaccagct ggtgcccggc gatattataa aactggcggc gggggatatg
    13561 atcccggcag atttacgtat cttgcaggcg cgggatctgt tcgtcgctca ggcgtcgtta
    13621 accggtgagt ctctgcccgt ggaaaaagcc gctactactc gccagccgga gcacagcaat
    13681 ccgctggagt gcgacaccct gtgttttatg ggcaccaccg tggtgagcgg cacggcacaa
    13741 gcaatggtga ttgctacagg agccaacacc tggtttggtc aactggcggg gcgtgttagt
    13801 gagcaggaaa gcgagccgaa tgcctttcag caagggatca gccgcgttag tatgctgctg
    13861 attcgcttta tgctggtgat ggctccggtg gtgttgctta tcaacggtta caccaaaggc
    13921 gactggtggg aagcggcact gtttgcgctt tcggtcgcgg taggcttaac gccggaaatg
    13981 ttgccgatga ttgttacctc gacgctggcg cgcggcgcag taaagctgtc gaaacagaaa
    14041 gtgatcgtca aacatctgga tgccattcag aactttggcg caatggatat tctgtgcact
    14101 gataaaaccg gcaccctgac gcaggacaaa attgtgctgg agaatcatac cgatatctcc
    14161 ggtaaaacca gcgaacgcgt gctgcatagc gcgtggttga acagtcatta ccagaccgga
    14221 cttaaaaatc tgcttgatac cgcggtgctc gaaggtacgg atgaagagtc agcgcgttcg
    14281 ctggccagcc gttggcaaaa aattgatgag attccgtttg atttcgagcg tcgtcggatg
    14341 tcggtggtag tggcagaaaa taccgaacac catcagctgg tttgcaaagg tgcattgcag
    14401 gaaatcctca atgtgtgttc gcaggtgcgt cacaatggcg agattgtacc gctcgatgac
    14461 accatgctgc gtaagattaa gcgggttact gatacgctga atcgtcaggg gctgcgcgtg
    14521 gttgcggtgg cgacgaaata cctgccagcg cgtgaaggag attaccagcg ggcggatgaa
    14581 tccgacctga tcctcgaagg atatattgct ttccttgatc cgccaaaaga gacaaccgct
    14641 ccggcactga aggcattaaa agcgagtgga attaccgtaa aaatccttac tggcgacagt
    14701 gagttagttg cagcgaaagt gtgccatgaa gtggggctgg atgcgggaga ggtggtcatt
    14761 ggtagtgata ttgaaacgct atctgacgat gaactggcaa atctcgcaca gcgcaccacg
    14821 ctgtttgccc gcctgacgcc gatgcataaa gaacgcattg tgactttact gaagcgcgaa
    14881 gggcatgtgg ttggctttat gggcgatggt attaatgatg cgcccgcctt acgcgctgcg
    14941 gatatcggca tttctgtgga cggcgcggta gatattgccc gtgaagcggc tgatatcatc
    15001 ctgctggaaa aaagcctgat ggtgctggaa gagggggtta ttgagggacg tcgcactttc
    15061 gccaacatgc tgaaatacat caaattgacg gcgagttcta acttcggtaa tgtgttcagc
    15121 gtgctggtgg cgagtgcttt cttgcccttc ctgccgatgt tgccgttaca cttgcttatt
    15181 cagaacctgc tgtacgatgt gtcacaggtg gcgatcccgt ttgataacgt cgacgacgag
    15241 caaattcaaa agccgcagcg ttggaatccg gcggatctgg ggcgctttat ggtcttcttc
    15301 ggaccgatca gctcgatttt cgatattctg acgttttgct tgatgtggtg ggtattccat
    15361 gccaacacgc cggaaacgca aacgctgttc cagtcgggct ggtttgtggt gggattactg
    15421 tcgcaaacgc tgattgtgca tatgatccgt acccgccgtg tgccgtttat tcagagctgt
    15481 gcatcgtggc cgttaatgat catgaccgtg atcgtgatga ttgtcgggat cgcattgccg
    15541 ttttcaccgc tggccagtta tctgcagctg caggcgctgc cgttaagcta tttcccgtgg
    15601 ctggttgcga ttctggcagg gtatatgacg ttaacccagt tggtgaaagg gttctatagc
    15661 cgtcgttacg gctggcagta aagaataaac tgggcatggt ggcccggatt cagattgcag
    15721 acaaagtgag ctttgttcat gccggatgcg gcgtaaacgc ccaatccggc ctacaaattc
    15781 aataaattgc aggaatcacg taggcctgat aagcgtagcg catcgggcga ttttgctttt
    15841 gtcatcagac ttaatccggg catgatagcc cggatttcca tcaagattag cgacgaacag
    15901 cgattgcttc gatctcaatc ttcacgtctt tcggcagacg ggcaacttca acgcaagaac
    15961 gtgccgggaa agtggcgttg tgttcggtga agaaggcttc gtaagtggcg tttacggttg
    16021 cgaagtcgtt cagatctttc acgaacacag tggttttaac gatgtcgccc actttcaggc
    16081 cagcggcttc gacgattgct tttacgttat ccagcgactg acgtgcctgt gcagcgacgt
    16141 ctgccggtac ttcaccagtt ttcggattta ccgggatctg accggaggtg atgatcatat
    16201 tgcccagatc aacgccctgt acgtaaggac cgatagctgc cggtgcattt tccgtcgcga
    16261 tagttttgct catgatttct cctttattac agcggtaaaa agtctggctc attataggga
    16321 gccagacttt tattaccaac cgcaattaat tggccagcac cacattatgg gaaaactctt
    16381 tttcacagta tttgcattta agcgcgatgt cattggcgcg ttttcgcacg gcaaagctgg
    16441 atgaaaccgg ttcggcatgg ctgatacagt tgctgttcgg gcagaccagc acattgtcga
    16501 tgcgctctgg cagacttggg cgcgatttac ccaccacttc atagttgtcg atacggttaa
    16561 ccgtggcttg cggtgcatac aatgccagtt ggtctacttg ctcttcactc aaaaaggtat
    16621 tttcgatttt gatcagatct ttgcggccca tctcgccaga aggcaaattc agaccaatgg
    16681 tgatgcgctg atctgtttcg gtcaacttga acagactcaa cagcttaaaa ccgatctggg
    16741 cggggatatg gtcaattacc gtgccgcgtt taatagcttc aacctgcaat ttattatcgt
    16801 gtgtcatctc tatttcccct tacagtacca gatcgcgatt cagaaccagt gccagtaacg
    16861 cctggcgagc gaaaatcccg ttgcctgctt gctggaagta ccaggcgtga ggcgttttat
    16921 ccacatccgt cgcaatctca tcaacacgcg gcagcggatg cagcactttc atattggctt
    16981 tggcgttgtg gagatcgctg gcgcgaagaa caaactgcgc tttcacgttg gcgtactcgg
    17041 acgggtccag acgctctttt tgcacgcggg tcatgtacag gatgtctact tccgccatca
    17101 cttcttcaat agagctgtgc agactccagg cgatcccttt ttcatcgagc atatccagaa
    17161 tgtattgcgg cattgccagc gcatccggcg cgataaagta aaaacggttg ccgtcgaact
    17221 tcgctagcgc ctgggtcagg gagtgaacgg tgcggccata tttcaggtca ccaaccattg
    17281 cgacgtggag attgtccaga cgcccctggg tttcctgaat ggtgaataag tccagcaagg
    17341 tttgcgtcgg atgttggttg gagccatcac cggcattcag taccggtaca ttgccagaaa
    17401 attcggtggc taggcgcgcc gcaccttcct gcggatgacg catcactatc gcatcgacgt
    17461 aagtgctgat aaccgaaatg gtatcggcca gcgtttcgcc ttttttaccc agtgatgtat
    17521 tggcgctgtc ggagaagccc accacgctgg cacccaggcg gtgcatggaa gtttcgaaag
    17581 agaggcgggt acgggtagag gcttcgaaga aacagctggc aatgactttg tgcttcaaca
    17641 gctctggttg cgggtttgct ttcagtttcg ccgctgtcgc cagcaccaga ttaaggtcat
    17701 cgcgactaag gtcgtttatg gaaatgatat gtttctgata tagcggatta gccatctttt
    17761 atctcctgac acctaggcaa aaaaaagccc ctcgattgag gggctgggaa tgggtgatca
    17821 acgggaagaa aaacggcagg ccagcgtctt ttttcagacg cggtaagaca aaatgtcgaa
    17881 cacactgaac catacatcct cccggcaaat tgtccggcat tatactcata gccagaagcg
    17941 gtgcaagcat ttgatgcaat attttgtcag cgcaaacggt ttatttgaat taaaagtcaa
    18001 aatatatgca tttttatttt ctatgattct ggaggggaag tgtcgttcgg tatcaataat
    18061 tgcagacatt tcctgctttt ttctttttca ttttcacgca atcagaaatg cattttattc
    18121 cattcatccg acttataggg cggagggtga aaacgaacgg ctaacactat tgcttatagc
    18181 tcagggatac gcgctatcac tttaatttca aaatcaaagc ctgccagcca ggtaacaccc
    18241 accgccgtcc agtttggata aggtggggcg ctaaatattt catttttcac cgtcatgatg
    18301 tcttcaaatt gtttttccgg atcggtatga aagctcgtaa catcaatgat atcgtcaaaa
    18361 gtgcatcccg cagctgccag ggtcgcatgc aaattatcaa aggccagtct gacttgttgc
    18421 tgaaaatcgg gttctggtgt tccgtcctct cgacttccta cttgcccgga aacaaacagc
    18481 aaatcgccgg aacgaatagc cgcagaataa cgatgctcag catacagtga atgtcggcca
    18541 gcagggaaaa cagcggttct ttctaccatt tggttatcct caagatttac gacatgaaca
    18601 gaagatttat atttatcggg agccgctttt agggggacga cgttagtaaa caaaacccag
    18661 acatcatgga taatggctgg gcttaattga gcgtagtcgg ttatgcgcca aacgcgccat
    18721 caatggtatg catcgcgccg gtaacaaaac tggcttctgg ccctgctaac catgcgacca
    18781 taccagcgac ctcttccggt tgcccatgtc ttttgatggc cataaaacta tgcaacatat
    18841 cgcgcattgg cccgttggcg ggattaacgt cggtatcaat tggtcctggc tggacgacgt
    18901 tgatggtgat ccctcgcggt ccaaaatcac gggccagccc gcgcgccatg ccttgcaggg
    18961 cagatttact ggcggcataa gcagccatgc ctgcaacagg catacgatcg ccattcacgg
    19021 agccgatgat taagatgcgc ccgccttcgg gcatctgccg ggcggcttca acagaggcat
    19081 gataaggagc atgaatattg attttgaaaa ggcgatcaat atcgtcggca tttaattcca
    19141 gggcatcgcc aaagacgcca atacctgcat ttaccaccag gatatccaat gcgccgctct
    19201 tacgaacgac atcaataaca gcgtctctgt cagcactatc tgtgaatact gctgtcgctc
    19261 cagtctcttg tgccagacgt tcagcggcat ctttcgaccc cgcataggtg aatcgtacat
    19321 tggccccatc ggtgacgaaa cgacgtacga tagcggcacc gattccacga ctgccaccga
    19381 ggatgagaac tgtcttaccg gtaaaagcgc ccataaggac tccttgattt attatgtaac
    19441 atgcattaca aaactgtttt aactttctgt caacaggttt tgtaatggtc actaaaaaac
    19501 aatctcgcgt tccaggtcgt cccagacgtt tcgctcctga gcaggcagtc tctgcggcaa
    19561 aagtgctttt tcaccaaaaa ggtttcgatg cagtaagtgt ctctgaagtt actgattatc
    19621 ttggtattaa ccccccgagt ctctacgcgg cttttggcag taaggctggg ttatttagcc
    19681 gtgtactcaa tgaatatgtc ggtacggaag ctattccgct tgccgatatt cttcgtgatg
    19741 atcgtccggt aggcgagtgc ctggttgagg tattaaaaga agcggcgcgc agatatagcc
    19801 aaaacggcgg ctgcgctggc tgtatggttc ttgaaggtat tcatagtcat gatccacaag
    19861 cgcgtgatat tgccattcaa tattatcacg ccgcagaaac gaccatttat gactatatcg
    19921 ccaggcggca tccacaaagc gcacaatgtg tgactgattt tatgagtacc gtgatgtcag
    19981 ggctttctgc gaaggcacgg gaggggcact ccatcgaaca actctgtgca acagctgccc
    20041 tggcagggga agcgataaaa actcttctca aggagtgatg ctggccttga tccgaaaggc
    20101 gggaacgcgc ctgccgataa gttgtgataa gacaataatt cacgcgttaa ggctggcgga
    20161 attgatcatc ttttcgtata acgatagaaa tgaaacatta ttttaattaa ggagtggaaa
    20221 agatgatcat cggaaatatt cataatcttc agccgtggct accccaggag ttacgccagg
    20281 cgattgagca tatcaaagca cacgttacgg cagaaacgcc aaagggcaag catgatatcg
    20341 aaggcaatcg tctgttttat cttatctcgg aagatatgac cgagccgtac gaagctcgcc
    20401 gtgcggagta ccatgcccgc tatcttgaca ttcagattgt gttaaaaggt caggaaggca
    20461 tgaccttcag cacgcaacct gcaggcgcgc cggataccga ctggttagct gataaagaca
    20521 tcgcattttt gccggaaggc gttgatgaga aaacagttat cctaaatgaa ggtgattttg
    20581 ttgtgtttta tccgggggaa gtgcataaac cgctgtgcgc agtgggcgcg ccagcccagg
    20641 ttcgcaaagc agtagtgaag atgctgatgg cgtgatgact tttcgccgta aataactcca
    20701 ggtttacggc gagtttgtga aaagagcgtt ttttgatatt tttttgtgag taaaatttgt
    20761 aatgcttaga cgttcttatt aactcaagga gttcgtcatg ggcaaaatat cagatttgaa
    20821 ttattctcaa cacatta
//
LOCUS       DAEEUE010000069        20724 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.069, whole genome shotgun sequence.
ACCESSION   DAEEUE010000069 DAEEUE010000000
VERSION     DAEEUE010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20724
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.069"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            81..350
                     /locus_tag="KLK84_002712"
     CDS             81..350
                     /locus_tag="KLK84_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBE2009980.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            344..709
                     /locus_tag="KLK84_002713"
     CDS             344..709
                     /locus_tag="KLK84_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBE2009981.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(710..1498)
                     /gene="bioH"
                     /locus_tag="KLK84_002714"
     CDS             complement(710..1498)
                     /gene="bioH"
                     /locus_tag="KLK84_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HBE2009982.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1536..2219
                     /gene="gntX"
                     /locus_tag="KLK84_002715"
     CDS             1536..2219
                     /gene="gntX"
                     /locus_tag="KLK84_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HBE2009983.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2278..2853
                     /gene="nfuA"
                     /locus_tag="KLK84_002716"
     CDS             2278..2853
                     /gene="nfuA"
                     /locus_tag="KLK84_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HBE2009984.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3214..4530
                     /gene="gntT"
                     /locus_tag="KLK84_002717"
     CDS             3214..4530
                     /gene="gntT"
                     /locus_tag="KLK84_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBE2009985.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="KLK84_002718"
     CDS             complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="KLK84_002718"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HBE2009986.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6669..9062)
                     /gene="malP"
                     /locus_tag="KLK84_002719"
     CDS             complement(6669..9062)
                     /gene="malP"
                     /locus_tag="KLK84_002719"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HBE2009987.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLNAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9674..12404
                     /gene="malT"
                     /locus_tag="KLK84_002720"
                     /pseudo
     CDS             9674..12404
                     /gene="malT"
                     /locus_tag="KLK84_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12447..13463)
                     /gene="rtcA"
                     /locus_tag="KLK84_002721"
     CDS             complement(12447..13463)
                     /gene="rtcA"
                     /locus_tag="KLK84_002721"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HBE2009988.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13467..14693)
                     /gene="rtcB"
                     /locus_tag="KLK84_002722"
     CDS             complement(13467..14693)
                     /gene="rtcB"
                     /locus_tag="KLK84_002722"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HBE2009989.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14882..16480
                     /gene="rtcR"
                     /locus_tag="KLK84_002723"
     CDS             14882..16480
                     /gene="rtcR"
                     /locus_tag="KLK84_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HBE2009990.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16462..17220)
                     /locus_tag="KLK84_002724"
     CDS             complement(16462..17220)
                     /locus_tag="KLK84_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HBE2009991.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17237..18067)
                     /gene="glpG"
                     /locus_tag="KLK84_002725"
     CDS             complement(17237..18067)
                     /gene="glpG"
                     /locus_tag="KLK84_002725"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HBE2009992.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18112..18438)
                     /gene="glpE"
                     /locus_tag="KLK84_002726"
     CDS             complement(18112..18438)
                     /gene="glpE"
                     /locus_tag="KLK84_002726"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HBE2009993.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18628..20133
                     /gene="glpD"
                     /locus_tag="KLK84_002727"
     CDS             18628..20133
                     /gene="glpD"
                     /locus_tag="KLK84_002727"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HBE2009994.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20402..20632)
                     /locus_tag="KLK84_002728"
                     /pseudo
     CDS             complement(20402..20632)
                     /locus_tag="KLK84_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         4894 a         5698 c         5230 g         4902 t
ORIGIN      
        1 gtcagtagat actctaccct gcttaagtgc tactttgtgc tatcttgcat gtaacacaaa
       61 gtagcactta gggaactgat atgcagacag tgaaattacg gcaacaagga ggagccatga
      121 tcgttactat cccacgagat cttgccatcg atcttggctg gtcaccgggc acagagctta
      181 ccgtggagaa aaaaggcgat agcgttaatt tgcgcgccac agagcacaaa ccgcgtgggc
      241 gtcttaccgt cgcgcagttg ttgagccaaa tcgatgaaag cgaaattacg gagctaaatc
      301 agtccacaga aggatgggct gaagggaaaa aaggtaatga agcatggtaa aggcacggac
      361 gccacatcgt ggtgagatct ggtattttaa ccctgatccg gttgccgggc atgaacttca
      421 ggggccacat tattgcattg tggtaacgga caaaaaactc aacaatgttt taaaagttgc
      481 tatgtgctgc ccgatttcaa caggggcaaa tgcagcacgt tccacagggg tgacggtgaa
      541 cgtcctcccc cgtgatacgc aaaccggtaa cctgcatggc gttgtacttt gtcaccagct
      601 aaaagccgtc gatcttattg cccgtggcgc taaatttcat accgttgccg atgaaaaatt
      661 gattagtgaa gttatcagta aactggtgaa tttaatcgac ccacaataat tagctctcac
      721 ttaccaccaa caccctctgc ttcaacgcca ccagcaggtg acaaaactcg accggatgcg
      781 aaataaacgg cgcatgggcc gctttggcga agatatatga ttcgctgtga ggccaaagtt
      841 tatccagcat cggcaccact ttgcgcggca ccagaccgtc gagatagcca tacaatcgca
      901 aaaacggcat ggacacgttt tgcagcggct gacggagatc gaccgttttc aggatttcca
      961 gcccgccatt aagcacgtca acctccggca tcggtaacgc cagaacggtt ttcttcaacg
     1021 cccgcgcatc ctggcgcgcc gtttcagtcc ccatggtttg taacgccagg aaccgctcca
     1081 ctgtacgctg aaaatcatca ctgagttgct gctgaaatcc cgccagcacg tccggtttta
     1141 tccccggcca ctcgtcacga gcactaaaac aaggtgacga cgccacggtg accagcgcct
     1201 gaacacgctc gggatgggtt aacgcaatct ggcttgccac cagcccgccc agactccagc
     1261 ctaaccaaat ggctttatca ggtgcctgtt gcagcacggc ttcggccata tcagcaagtg
     1321 acagcgcacc aaatccccgg ctacgcccga agccgggcag gtcaacaagg tgcagcgtaa
     1381 aatgcgagct aagttcctcg tcaatgcaac gccacacttc ggcattcagt ccccatccgt
     1441 gcagcagcac aagatgaaca ttcccctgac ctttggtctg ccaccagatg ttattcatcc
     1501 gctattgttc tcttttgact tacaaggatg aacatatgct aacagtaccg ggattatgct
     1561 ggctatgccg aatgccactg gcgttaggtc attgggggat ttgttcggtc tgttcacgcg
     1621 ccacccgcac agataaaacg ttatgcccac aatgtggatt acccgccaca cactcccatc
     1681 ttccctgcgg tcgctgcctg caaaaaccgc cgccctggca aagactggtc acggttgccg
     1741 actatgcgcc gccgttaagt ccgcttatcc accagcttaa attttcccgg cgcagtgaaa
     1801 tcgccagcgc cctgtcacgt ctgttactat tggaagtctt acacgctcgt cgcaccaccg
     1861 ggttgcaatt gccggatcgc atcatcagcg ttccgttatg gcagcggcgt cactggcgtc
     1921 ggggatttaa tcagagcgat ttgctgtgtc agccgttatc acgctggttg cactgccaat
     1981 gggatagcga agccgtcaca cgtacacggg ccactgcgac ccagcatttt ctcagtgccc
     2041 ggctgcgcaa gcgcaacctg aaaaatgcct ttcgtcttga attgcccgtg caaggtcgcc
     2101 atatggtgat tgtggatgat gtcgttacca ccggaagtac cgtcgcagag attgcgcagt
     2161 tgcttttacg caatggtgcg gcgactgtcc aggtctggtg cctttgtcga accttgtaga
     2221 gcctcgatga tgggcgtatt ataaccaact aaaatagtca actattaggc cattactatg
     2281 atccgtattt ccgatgctgc acaagcgcac tttgccaaac tgctggcaaa tcaggaagaa
     2341 gggacacaaa tccgcgtatt tgtgattaac cctggcacgc ctaacgctga atgtggtgtt
     2401 tcttattgtc cgccggatgc cgtggaagcc accgacactg ccctgaaatt tgacctgctg
     2461 accgcgtatg ttgatgagtt aagcgcacca tacctggaag atgcagagat cgactttgtt
     2521 accgaccagt tgggttctca gttaacgctg aaagccccga acgccaaaat gcgtaaagtg
     2581 gcagacgatg caccgctgat ggagcgcgtg gagtatatgc tgcagtcgca gatcaaccca
     2641 cagcttgctg gtcacggtgg tcgcgtttcg ctgatggaaa tcaccgaaga cggttacgcc
     2701 attctgcaat ttggcggcgg ctgtaacggt tgttccatgg tcgatgtgac gctgaaagaa
     2761 gggatcgaga agcagctgct gaacgaattc ccggagctga aaggtgtgcg cgatctcacc
     2821 gaacaccagc gcggcgaaca ctcctactac taagttatcc cctcttttgt agattgcccg
     2881 atgcgacgct aaagcgtcat ttcgggccta cgttgaacat gtgccagacg ttggcacagc
     2941 attgctttgg caaatccctc ccttcgtttc cccgcgataa tatgaccaac ctctcataat
     3001 ttaaatttac cccgctctgg tgattctcaa acgccaaatg ttacccgtat cattcacatg
     3061 ggtaccaaac atactcctga catctgacta caataattag ttttagtggg tatcagtcgt
     3121 ggtgccgcaa tatctctgtt cccgattggg ataattagag tttgtcgtca gaaaattgac
     3181 gttacccata acaaatgaaa ggccaggtaa atcatgccat tagtcattgt tgctatcggt
     3241 gtaatcttgt tgttgctcct gatgatccgc ttcaaaatga acggcttcat cgctctcgtc
     3301 ctcgtggcgc ttgctgttgg attaatgcaa ggaatgccgc tggataaagt tattggctcc
     3361 atcaaagccg gtgtcggcgg gacgctcggt agccttgccc tgatcatggg ttttggcgca
     3421 atgctgggca aaatgctggc agactgcggt ggcgcacaac gtatcgccac cacgctgatt
     3481 gccaaatttg gtaaaaagca catccagtgg gcggtggtgt taaccggttt taccgttggt
     3541 tttgccctgt tctatgaagt gggcttcgtg ctgatgctgc cgctggtgtt taccatcgcg
     3601 gcttccgcga atattccatt gctgtatgtt ggtgtaccaa tggcggctgc actgtctgtg
     3661 acccacggct tcctgccacc gcatccgggc ccgactgcga ttgccaccat tttcaatgcc
     3721 gatatgggta aaaccctgct gtacggtact attctggcaa tcccgaccgt gattctcgcc
     3781 ggtccggttt acgctcgcgt gttgaaaggt atcgataagc caatcccgga aggtctctac
     3841 agcgcgaaaa ccttcagcga agaagagatg ccgagctttg gcgtcagcgt ctggacctct
     3901 ctggtgccgg ttgtgctgat ggcgatgcgt gcgattgccg aaatgatcct gccgaaaggt
     3961 cacgctttcc tgccggtagc ggagttcctc ggtgacccgg taatggcaac gctgattgcc
     4021 gtgctgattg cgatgttcac ctttggtctg aaccgtggtc gttcaatgga tcagattaac
     4081 gacacgctgg tttcttccat caaaatcatt gcgatgatgc tgttgatcat cggtggtggc
     4141 ggtgcgttca agcaggtgct ggtagacagc ggcgtggaca aatacattgc ttccatgatg
     4201 cacgaaacca acatttctcc gctgctgatg gcctggtcga ttgctgccgt actgcgtatc
     4261 gcgctgggtt ccgcaaccgt tgcggcaatc actgcgggtg gtatcgcggc accgctgatt
     4321 gcaacgacgg gtgttagccc agagctgatg gttattgcgg tgggttccgg tagtgtgatt
     4381 ttctctcatg tgaacgatcc gggcttctgg ctgttcaaag agtactttaa cctgactatc
     4441 ggcgagacca tcaaatcctg gtcgatgctg gaaaccatta tctcggtgtg cggtctggta
     4501 ggctgtctgc tgctgaatat ggtgatttga taacactgac tgccggatgc atcgcatccg
     4561 acatcaaccg cactctactt cttcttcgct gcagctctgc gccgtctgtc caaatccttc
     4621 agcaacttgt tcacgccatc atcggcaaac atcgactcaa gcgttgcgga aagcttgcgt
     4681 cgccagtttt tatactggta actggtgcca ggaatattca ccggttcggc catatccagc
     4741 cagtcttccg gctgtagtcc taacagagca ctgttactgt cggcaatgta gcgctgcaaa
     4801 ccacggttca gcgtcggcgt catcgacatc aacgatgcct tatgcccggc acgttttggc
     4861 agacaaccat atttatgcag tgcatccagc agcccttgct tcgccagttc gcgatcctga
     4921 tacagaccgc gcagtaccac ttcatccgga tacagcccca gggttttgcc cagcgttaga
     4981 tccccgctct cccagtaacc acgcagcgtt ggcaggtcat gtgtcgccgc aaccgccatc
     5041 gactgctccg gatacgcttt cggtgcacgg aacgtcttct cgtggtcgtt ttcgaaatag
     5101 agcactttgt aagagtacac accgctgctg cgcagcttac caacaatctc taccggtacg
     5161 gtaccgagat cttcaccaat caccatacag cgatgacgtt tactttcgag tgccagaatc
     5221 gagagcagat catccaccgg atagtgaaca tacgcgccct gatctgccgt ctcgccatac
     5281 ggtatccacc acaaacgcag catcgacatc acatggtcaa ttcgtaatgc gccgcagttt
     5341 tgcatattgg cacgcaacag ctcgataaac ggttcatagg cacgcgcggt gatgatatgc
     5401 gggtccattg gcggtaatcc ccagttctgc cccaacgggc cgaggatatc cggcggcgcg
     5461 ccaaccgatg ctttcaggca atatagttca cggtcacacc aggtttccgc cccaccttcc
     5521 gctacgccaa ccgccagatc acgatacaag ccaatcggca tttcatagcc ctggcttatc
     5581 tcccagcagg cggcaaactg gctgtaagcc agccactgca accagagata aaaatcgacg
     5641 tcatcacgat gttcttcgca gaactgacgc acttctggtg aatccacgtt cttatacatc
     5701 tctggccatg caggccagcc ccagcgcatt tcgtcctctt tcacttgctg ggcatgtagc
     5761 gcatcaaagg cagcctgcca gaacaggctg tcgccctgct ctgcaacaaa ctggcgaaac
     5821 gcggccatct gctcatcatc acgttgcgcg aaacctttcc acgccattcg taatgctgtc
     5881 atttttaggg cggtaaccgt ggagtaatcg acccagtcgg catcgcgcgc ctgttgcagc
     5941 gtctgttgcg tggtcggcaa ctgccaccag gcctgagcct cttcgctaag atggaaatct
     6001 tcaacggcgt taacgtcgat ataaatcaca ttcagccaac ggcgagaaga cgggctgtat
     6061 gggctggcgc tctccggatt tgccggatag agcgcatgaa tcgggttcag gccaatgaac
     6121 gacccgccac gttttgccac atccaccagc atcgctttga gatcgccaaa atccccaata
     6181 ccccagtttt tttccgatcg cagcgtataa agctgaacgc aggcacccca cagcttttgt
     6241 ttattcagca aagcctgcgg ttcgtaacaa cgtttcgggg cgacaatcac ccggcaatgc
     6301 gcgcgctggt cgtcctgggt gagtgttagc gtgtgataac cttccggcag cttcgtcggt
     6361 agattgaacg ctttgccccc cgttacatgg cctttgtact gcgttccttc ttcggtggtc
     6421 agctgccagc tatattcgcc gctgccctcc accaccatcg gcattttttt gccgctggta
     6481 taaaccatga cattcgggac tggcgttacc gccactttcg tggcggtacg ttgatgcatc
     6541 gcgtcaagca aacgccgttt ggtttcggcg ctaatcgact gcggtttacc gtgggcattg
     6601 atgtaattgg ggctaatccc cgccgccagc gcggcattat ccagacgttt gctttccatc
     6661 gagcttcctt agcgttttgc ctgccagata cgagcctgat aatcgcgaat agagcgatcc
     6721 gagctaaaca taccgcagcg ggcggtattg aggatcgccg cgcgagtcca ggcctcctgg
     6781 tcgcggtaca gcacatccac ctgcttttgt gcctcaacat aggctgcgaa atccgccatc
     6841 accagatacg gatcgccgcc ctgtttgccg atactgtgca gcatctggtc gaaggcatgc
     6901 ttatcgccgt cgctgtattt accgctttcc agctctttca atactgcgtt cagcacctta
     6961 tctttcttcc gccatttcac cgggtcgtag cctttggcca gaatagcctt cacttgttcc
     7021 acggtatgac caaaaataaa gatattttct tcaccgactt tctcggcgat ttcaacgttc
     7081 gcaccatcca gcgtaccgac agtaagcgca ccattgagcg ccagtttcat attgccggta
     7141 ccggaagctt ctttacctgc agtcgaaatt tgttcggaga tatccgccgc cgggatcagt
     7201 ttttccgccg ccgaaacgca ataatccggc aggaacacca ccttcaactt atcgccaacc
     7261 agtggatcgt tgttgatcac gtcagccact ttgttgatcg caaagataat attcttcgcc
     7321 aggtagtagc ccggtgccgc tttcgcgccg aagaggaaga cgcgcggtac gcgatcagcc
     7381 tgcaggtttt cacgaatttc tttgtacaac gccagaatat gcagcagatt caggtgctgg
     7441 cgtttgtact catgcagacg tttgatctga atatcgaaaa tcgcctgtgg attgatctca
     7501 ataccggtac gaactttcac aaactccgcc agacagactt tattcgcctg cttgatctcg
     7561 cgatattgct gacggaattt cgcatcatca gcgaattttt ccagattgat cagctgatcg
     7621 agatcgttag cccactcttt ttgcagtgat ttatccaaca gagccgccag tgcagggttg
     7681 cactgtttga tccagcgacg tggggtaata ccgttggtaa cgttatggaa tttgttcggc
     7741 catagctggt gatattccgg gaacagatct ttcaccacca gatccgagtg cagcgccgca
     7801 acaccgttca ccgcgaaacc gccaaccaca cacaggttcg ccatatgcac ttgtttatcg
     7861 tgcaccaccg ccagtttggc ccacactttt tcatcgcccg gccaggtttt ctccaccagc
     7921 gttttaaagc gagtattaat ttcgttaata atctgcatat ggcgcggcag taagcctttc
     7981 accagtttca catcccagcg ttccagcgct tctggcatca gggtatggtt ggtgtaagcg
     8041 aaagttttgc tggtgatggc ccaggcgtca tcccagctca tctggtgctc atcggtcagc
     8101 acgcgcagca gttctggaat cgcgatagtt ggatgggtat cgttcagctg aataacttcg
     8161 taatccgcca gttcgtgcag tttacgcccc gccagatgat ggcgacgcaa aatatccgct
     8221 accgaacagg cacactggaa gtattgctgc atcaggcgca gctttttacc ggcagtatgg
     8281 ttgtcgtttg gatagagaac tttggtcagt ttttccgcat tgatgccttg ctgttcggcg
     8341 cgcaggaaat caccgtcgtt aaatttagtt agatcaaacg gatgcgcatg cgtcgcctgc
     8401 cacagacgca gcggctgcgc cacgccatta cgatagccga caacggggag atcccacgct
     8461 tgaccggtaa tggtaaactc cggctcccag cgtccgtctt tcatcacttt accgccaatc
     8521 cctacctgca catccagtgc ttcgttgtgg cggaaccacg ggtagttact gcgatgccag
     8581 tcatccggcg cttcaacctg tttgccatcg acaaaagact ggcggaacaa accatattga
     8641 tagttcagac cgtaacccgt cgcagactga ccgacagttg ccattgagtc gaggaagcac
     8701 gccgccagac gtcccagacc accgttaccc agcgccgggt cgatctcttc ttccagcagg
     8761 tcagtcaggt tgatgtcata agccttcaac gaatcctgta catcctgata ccagccgaga
     8821 ttcaacaggt tgttgcccgt cagacgacca atcaaaaact ccattgagat gtagttaaca
     8881 tgtcgctgat tcgccaccgg tttggcgaat ggctgagcac gcagcatttc ggccagtgct
     8941 tcactcactg ccagccacca ctggcgagga gtcatttcag ccgcagaatt taagccataa
     9001 cgctgccact gacgtgaaag cgcttcctga aattgcttat cgttaaaaat aggttgtgac
     9061 ataggagttc cacttttctt agattttcaa cacaacgtta tcgctagttt gccaggctcg
     9121 atgttgacct tcctcatcct gcgggggatt aggcagggag gagttgcggg gatgagcaag
     9181 gaaatgtgat ctcgaccact taaagctagt gcaaaccaca ggattagcag caaatcaatg
     9241 ccatacagcg cagaaaatct gtatctaagt gcaaaaaatg gccgttgcgt attttcaaaa
     9301 agcggaaggt aactctataa attaagtaaa ggagtgaaac agtttcacaa gtaaaatatc
     9361 cagtgtgctc catctcattc ttaatagatt tattaagatc atctttttag atggcacttt
     9421 catcaggaat gaagaagaaa cccttgctta aatgaatctg atgaacataa gggaaaccag
     9481 tattcacgct ggatcagcgt cgttttaggt gagttgttaa taaagatttg gaattgtgac
     9541 acagtgcaaa ttcagacaca taaaaaaacg tcatcgcttg cattagaaag gtttctggcc
     9601 gaccttataa ccattaatta cgaagcgcaa aaaaaataat atttcctcat tttccacagt
     9661 gaagtgatta actatgctga ttccgtcaaa actaagtcgt ccggttcgac tcgaccatac
     9721 cgtggttcgt gagcgcctgc tggctaaact ttccggcgcg aacaacttcc ggctggcgct
     9781 gatcacgagt cctgcgggct acggaaagac caccctcatt tcccagtggg cggcaggcaa
     9841 aaacgatatc ggctggtact cgctggatga aggtgataac cagcaagagc gtttcgccag
     9901 ctatctcatt gccgccgtgc agcaggcaac caacggtcac tgtgcgatat gtgagacgat
     9961 ggagcaaaaa cggcaatatg ccagcctgac gtcactcttc gcccagcttt tcattgagct
    10021 ggcggaatgg catagcccac tttatctggt catcgatgac tatcatctga tcactaatcc
    10081 agtgatccac gagtcaatac gcttctttat tcgccatcaa ccagaaaatc tcaccctggt
    10141 ggtgttgtca cgcaaccttc cgcaactggg cattgccaat ctgcgtgttc gtgaccaact
    10201 gctggaaatt ggcagtcagc aactggcatt tacccatcag gaagcgaagc agttttttga
    10261 ttgccgtctg tcatcgccga ttgaagccgc agaaagcagt cggatttgcg atgacgtttc
    10321 cggttgggcg acggcactat agctaatcgc cctctccgcc cggcagaata cccactcagc
    10381 ccataagtcg gcacgccgcc tggcgggaat caatgccagc catctttcgg attatctggt
    10441 cgatgaggtt ttggataacg tcgatctcgc aacgcgccat tttctgttga aaagcgccat
    10501 tttgcgctca atgaacgatg ccctcatcac ccgtgtgacc ggcgaagaaa acgggcaaat
    10561 gcgtctcgaa gagattgagc gtcaggggct gtttttacag cggatggatg ataccggcga
    10621 gtggttctgc tatcacccgc tgtttggcaa cttcctgcgc cagcgctgcc agtgggaact
    10681 ggcggcggag ctgccagtgg gaactggcgg cggagctgcc ggaaatccac cgtgccgccg
    10741 cagaaagctg gatggcccag ggatttccca gcgaagcgat tcatcatgcg ctggcggcag
    10801 gcgatgcgct gatgctgcgc gatattctgc ttaatcacgc ctggagtttg ttcaaccata
    10861 gcgaactgtc gctgctggaa gagtcgctta aggccctgcc gtgggacagt ttgctggaaa
    10921 atccgcagtt agtgttattg caggcgtggc taatgcaaag ccaacatcgc tatggcgaag
    10981 ttaacaccct gctagcccgt gctgaacatg aaatcaagga catcagagaa ggcaccatgc
    11041 atgcagaatt taacgctctg cgcgcccagg tggcgattaa cgatggtaat caggatgaag
    11101 cggaacggct ggcaaaactg gcactggaag agctgccgcc gggctggttc tatagccgca
    11161 ttgtggcaac ctcggtgctg ggtgaagtgc tgcactgcaa aggcgaattg acccgctcac
    11221 tggcgctagt gcagcaaacc gaacagatgg cacgccagca cgacgtctgg cactacgcct
    11281 tatggagttt aattcagcaa agtgaaattc tgtttgccca agggttcctg caaaccgcgt
    11341 gggaaacgca ggaaaaagcg ttccagctga tcaacgagca gcatctggaa cagctgccaa
    11401 tgcatgagtt tctggtgcgc atccgagcac aactgttatg ggcctgggca cggctggatg
    11461 aagccgaagc gtcggcgcgt agcgggattg aagtcttgtc gtcttatcag ccacagcaac
    11521 agcttcagtg cctggcaatg ttgattcaat gctcgctggc ccgtggtgat ttagataacg
    11581 cccgtagcca gttgaaccgt ctggaaaacc tgctggggaa tggcaaatat cacagcgact
    11641 ggatctctaa cgccaacaaa gtccgggtga tttactggca aatgaccggc gataaagccg
    11701 ccgctgccaa ctggttgcgt catacggcta aaccggagtt tgcgaacaac cacttcctgc
    11761 aaggtcaatg gcgcaacatt gcccgcgcac aaatcttgct gggcgagttt gaacctgcgg
    11821 aaattgttct cgaagaactc aatgaaaatg cccgcagtct gcggttgatg agcgatctca
    11881 accgtaacct gttgctgctt aatcaactgt actggcaggc cggacgtaaa agtgacgccc
    11941 agcgcgtgtt gctggacgca ttaaaactgg cgaatcgcac cggatttatc agtcattttg
    12001 tcatcgaagg cgaagcgatg gcgcaacaac tgcgccagct gattcaactt aatacgctgc
    12061 cggaactgga acagcatcgc gcgcagcgta ttctgcgaga aatcaatcaa catcatcggc
    12121 ataaattcgc ccatttcgat gagaatttcg ttgaacgtct gctaaatcat ccggaagtgc
    12181 cagaactaat ccgcaccagc ccgttgaccc aacgtgaatg gcaggtcctg gggctgatct
    12241 actccggtta cagcaatgaa caaattgcgg gcgaactgga agtggcggca accaccatca
    12301 agacgcatat ccgcaatctg taccaaaaac tcggcgtcgc ccaccgtcag gctgccgtgc
    12361 agcacgcgca gaaactgctg aagatgatgg gttatggggt gtgagtttag ccggataatg
    12421 cattggatcc ggtttacatc tttacatcat tcaatactca cccgcgttac gccatctgtt
    12481 tctatcaaac caaaccgcac cggcaagaaa cgctccacca ccgcgatatt ggtcagcaga
    12541 tggcatgaag gatgggcgac cgtaaattct cccgcgcccg ccagcgccat cggcagtacc
    12601 agttggtcgg cgagatattc ccccaccgcc gctgggcttg ccaggtagcg tttcacctct
    12661 ttcactaact gcgcggctac cacctcggca ctgacgcgct tttcaccgac gacgaaaaag
    12721 cgttcagtga tattttcact ttcgacttcc agcgagacgg tattacccgg cccctggtcg
    12781 cgcggcaggt tatgaatatt ctgttcatgc agggaaaaaa tccccgccag tgtagcgatt
    12841 tcacgctcag caacatggcg cggcacacca gctaatagaa cttctccacg catctgcaca
    12901 atgttcccgc gctcgccaag ttgcaaggtg ctaaacgatg ccacgggcga gacttccgtt
    12961 gccaccacac cgcctccggc aggataaaaa ccgtgacgta acagcgtggt ttgctgatga
    13021 attcctattt tcgccagcag cggctccagc acccggcgga taaaatccgc aggcggcgcc
    13081 gacgggttat cggtgccgcc gctcacttca acacgcgaag gtccatcggc aaaccacagc
    13141 gcgggcagca ccgtttgcag caccagcgta caacttccgg cgctaccgat agcaaagcgg
    13201 taatcgccgc cgcgcacggt gccaggccgg aagaccagac gctgcgatcc cagctccgcg
    13261 ccttccaccg ttgccctaca aatttccgca gccgctttta ccgcggtcag atgctggcgc
    13321 agcagccccg gtttcgcccg tccggctcga atgccagtga tggtaaatgg ctggccggtt
    13381 atcatcgaca ggctcagcgc cgagcgcagg atctgcccgc cgccttcgcc ctgtgcacca
    13441 tccagcgcaa tcatcctttt catcatttat ccttttacgc acaccacctg acgcagggtg
    13501 tagataactt ccaccagatc gctttgtgcc gccatcaccg catcaatatc tttatacgcc
    13561 atcgggattt cgtcgatcac ttcggcatct ttacggcatt ccacatgcgc ggtggcacga
    13621 atttggtctt ccacgctgaa cagttttttc gctttagttc ggctcattac ccgcccggca
    13681 ccgtggctgc acgaacagaa cgactcttca tttcccagcc cacggacgat aaagcttttt
    13741 gctcccatcg aaccgggaat aattccatat tgaccagcac gcgcagacac cgcaccttta
    13801 cgcgtcacgt agatctcttc accaaagagc tgttcttttt gcacatagtt gtggtgacag
    13861 ttgatctctt ccatcgccag cgtttgtggc tgtctgaccg ttttctgcgt aatgctctgc
    13921 aacgccgtta ccacattttc catcatcgca tcgcggttaa ggctggcgaa aagctgcgcc
    13981 caggccacgg ctcttaggta atcatcaaag tattccgtac cttccataaa gtacgccaga
    14041 tcacgcgacg gcaacgtctc aagttggtcc tgcatctctt tttgcgccag atcgataaag
    14101 taagtcccga tggcgttacc aattccgcgt gaaccggagt gcagcataat ccacacctgc
    14161 tccgactcat caaggcagat ttcaataaag tggttacccg ttcccagcgt tcccaggtgt
    14221 ttatagttgt tagtgttaag gaaacgcgga tatttttgcg ttaaccactg ataaccggcc
    14281 tccagctcag cccatttcgc atcgacgtta aaaggcgggt tttcccatgc gcctttatca
    14341 cgtttacaac gcccggtagt acgcccgtgt ggtacggccg tttcaatcgc ttgacgcagc
    14401 tccgccaggt tttcaggcag gtctgccgcc gttaacgcgg tacgcagcgc gttcattcca
    14461 cagccaatat ccacgcccac cgccgccgga ataatcgccc ctttggtcgg gatcacgcta
    14521 ccaatggtgg aacctttacc caggtgtaca tcaggcatta ccgcaatatg tttgaaaata
    14581 aacggcatct tcgccgtatt aataagttgc tgacgcgcat cggcttctac cggcacgcct
    14641 ttggtccaca tttttaccgg ggcattttca gtggtcagta attcgtaatt cattttgttt
    14701 ttctcttttc gttgtttgcg gtcctgataa ttgcaaccgt cgtgccagaa aattaaataa
    14761 gcagattaat tttttaattt attgtttcta aaaagattat atctttatgt ccgtaaacgg
    14821 agatttcccg caaagccgat ttaccgataa tgaaatatcg ccttttataa ggatatctaa
    14881 gatgcgtaaa acagtggctt ttggctttgt cggtaccgtg ctggattatg ccggacgcgg
    14941 cagtcagcgc tggtcaaaat ggcgtccgtc actctgtata taccagcaag aatcgttggt
    15001 catcgatcga ctggaattat tgcacgatgc ccgctcgcgc tcgctatttg aaacgcttaa
    15061 acgcgatatc gccagcgttt cgccagaaac agaagtggtg ggcgttgaga ttgaactgca
    15121 taacccgtgg gatttcgaag aggtttacgc ctgcctgcat gatttcgccc gtggttacga
    15181 gtttcagcca gaaaaagaag actatttaat tcacatcacc accggcaccc acgtcgcgca
    15241 gatttgctgg tttctgctgg cggaagcgcg ttatctaccc gcccggttga tacaatcttc
    15301 aactccgcgc aaaaaagaac agccgcgcgg cccaggtgaa gtgacgatta tcgatctcga
    15361 tttaagccgt tataacgcca tcgccagccg ctttgccgag gaacggcagc aaacgctcga
    15421 tttcctcaaa tccggtattg ccacgcgtaa cccccacttc aaccgcatga ttgagcagat
    15481 cgaaaaagtg gcgattaaat cccgcgcgcc gattctgctt aacggcccga ccggcgcagg
    15541 taagtcattt ctggcgcgac gcatcttcga gttaaaacag gcacggcatc agtttagcgg
    15601 cgcgtttgtg gaagtgaact gcgccaccct gcgcggcgat accgcgatgt cgacgctgtt
    15661 tggtcatgtg aaaggcgcgt ttaccggggc gcgggaatct cgtgaaggat tattacgcag
    15721 cgccaacggc ggaatgttgt ttcttgatga gattggcgaa ctgggcgcag acgaacaggc
    15781 aatgctgctg aaagctattg aagagaaaac cttttacccg tttggcagcg atcgccaggt
    15841 gagcagtgat tttcagctta tcgccggaac ggtgcgcgat ttgcgccagc tggttgccga
    15901 aggcaaattc cgcgaagatc tgtacgcgcg gatcaatctc tggaccttca ccctgccggg
    15961 tctacgccag cgccaggaag atattgaacc gaacctggat tatgaagtgg agcgccacgc
    16021 cacactcact ggtgacagcg tgcgttttaa caccgaagcg cggcgtgcct ggctggcctt
    16081 tgcaacatct ccccaggcaa catggcgcgg taactttcgc gaactttctg ccagcgtcac
    16141 gcggatggcc acctttgcca ctagcggacg catcactctg gacgtggtgg aagatgagat
    16201 aaaccgtctg cgctataact ggcaggagag ccgcccctcc gcgcttacgg cgttgctggg
    16261 cgcagaggca gaaaacatcg atctcttcga ccgtatgcaa ctggaacacg ttatcgctat
    16321 ctgccgccag gcaaagtcgc tttctgctgc cggacgacag ctttttgatg tttcgcgcca
    16381 gggcaaagcc agcgtcaatg acgcggatcg gctacgcaaa tacctggcgc gttttggtct
    16441 gacgtgggaa gccgtgcagg atcagcacag ctccagttga atatggtggt ccgtcagcac
    16501 ctgcatcacg cttactggcg gcggggcgtc ggtgtagacg gcatcaacca tgctgatgct
    16561 ccccatattg accatcgcat tacggccaaa ttttgaatgg tcaaccacca gcataacgtg
    16621 gcgcgagttc tcaataatgg cgcgcttggt acgaacttcg tggtaatcga actccagcag
    16681 cgagccgtcg ctgtcgatgc cgctgatccc cagaatgccg aaatcaaggc ggaactggga
    16741 gataaaatcg agcgtcgctt cgccaatgat cccgccatcg cggctacgta attcgccacc
    16801 ggcgagaata atgcggaaat cttcttttac catcaacgtg ttagcaacgt tgagattgtt
    16861 ggtgacaatg cgcaaattgc tgtgattgag cagcgcgtgc gccaccgctt ccggcgtggt
    16921 gccgatatcg ataaacagcg tcgagccatt ggggatttgc tccgccactt tgcgggcgat
    16981 gcgctctttt tcttcggtct gggtggcttt acgatcgtgc cacggcgtgt taaccgaact
    17041 ggaaggcagc gccgcgccgc catgatggcg caggatcagg ttttgctctg ccagctcgtt
    17101 gaggtcgcgg cgaatagtct gcgggctgac ggagaagtgc tccaccagtt cctcggtact
    17161 gacataaccc tgctgtttaa ccagttcgat aataccgttg tgacgttgtg tttgtttcat
    17221 ttataaatcc ctggaattat tttcgttttc gcgcattgag cgaatcaaca aaagccatcg
    17281 ctaaacccac ggctaatccg gcgatgtgtg ctccgttcgc catcgacatc ccaaacaaat
    17341 caaaccatcc ggcgacaatc cagatcagcg caaagataat taacccacgt tgcaggtaaa
    17401 tgccactttg cggatcgcgt tcgccacgta gccagacgta gcccatcagc gcatacacca
    17461 cgccagaaag cccgccaaac cacggcccgc tgaatttttg ctgcatatag ccgcttaaca
    17521 gggcgctgat aagcgtaatg acaattagct taccgctacc gaggcgtttt tccaccgcgc
    17581 cgccgagata ccaccaccaa agcaggttaa agaggatatg catcagcgag aagtgcatta
    17641 acgcgtgggt gaagtaacgc cagaactcaa atttcagtgt tggatcgaat ggccaggcca
    17701 gccataacat cacttcctga tcgccgagaa tttgcatggc aataaacacc accacgcagg
    17761 cgatcatcat cacccaggtc accggacctg cgcgttcacg caaggcagca aagaaaggat
    17821 aacggcgata atgcaggcca ctgccggtat ggcctgccag ccagctggcc gccagataac
    17881 gcggatctgc cgggttttcg agaaaacgcg ccagctccgc ccgcacgcgc tcggcctggg
    17941 actcatccgc cagccagaca tcgctttggt tatgttgttg aatcgtgagg ataacaccct
    18001 gcgtcgccat gtaatcaaca aacgcctgcg ccacgcgggg gttagcaaaa gaggtaatca
    18061 tcaacatcgt tgctgtcgct tattccacac aaaaggggac agtataaagc gttacgcgcc
    18121 gtacgccacc tctgcgggaa actgtcgctg ccaggcttca aagccgccgt caatgctata
    18181 gaccacatcg tagccctgtt gcagcagata ctgcgccgcc cctttgctgc tattgccgtg
    18241 ataacacatc accatcaccg gagtgtcaaa gtcgttatca cgcataaacg cgcccagcgt
    18301 gtcgttggtt aaatggaaag cctgcaccgc atgccccata gcgaaactct gtggatcgcg
    18361 aatatcgacc agcaccgcct ctttttcctg caacttctgg tgcgcgtcgg caacgttaat
    18421 acattcgaac tgatccatgc gtctctcttt ctttacaaac aagtgggcaa atttaccgca
    18481 cagtttacgt cgaagcggca gataaacgcc ataatgttat acatatcact ctaaaatgtt
    18541 ttttcaatgt tacccaaagc gcgattcttt gctaatatgt tcgataacga acatttatga
    18601 gctttaacga aagtgaatga gggcagcatg gaaaccaaag atctgattgt gataggtggc
    18661 ggcatcaatg gtgctggtat cgcggcagac gccgctggac gcggtttatc cgtgctgatg
    18721 ctggaggcgc aggatctcgc ttgcgcgacc tcttccgcca gttcaaaact cattcacggt
    18781 ggcctgcgct accttgagca ctatgaattc cgcctggtca gcgaggcgct ggctgaacgt
    18841 gaagtgctgc tgaaaatggc tccgcatatc gccttcccga tgcgttttcg cctgccacat
    18901 cgtccgcatc tgcgcccggc gtggatgatt cgcattggtc tgtttatgta cgatcatctg
    18961 ggtaaacgca ccagcctgcc gggatcaact ggtttgcgtt ttggcgcaaa ttcagtgtta
    19021 aaaccggaaa ttaagcgcgg attcgaatat tctgactgtt gggtagacga cgcccgtctg
    19081 gtactcgcca acgcccagat ggtggtgcgt aaaggcggcg aagtgcttac tcggactcgc
    19141 gccacctctg ctcgccgcga aaacggcctg tggattgtgg aagcggaaga tatcgatacc
    19201 ggcaaaaaat atagctggca agcgcgcggc ctggttaacg ccaccggccc gtgggtgaaa
    19261 cagttcttcg acgaggggat gcatctgcct tcgccttatg gcattcgcct gatcaaaggc
    19321 agccatattg tggtgccgcg cgtgcatacc caaaagcagg cctacattct gcaaaacgaa
    19381 gataaacgca ttgtgttcgt gatcccgtgg atggacgagt tttccatcat cggcaccacc
    19441 gatgtggaat acaaaggtga tccgaaagca gtgaagattg aagagagtga aatcaattac
    19501 ctgctgaaag tgtataacgc gcactttaaa aagcagctaa gccgtgacga tatcgtctgg
    19561 acctactccg gtgtgcgtcc gctgtgtgat gatgagtccg actcgccgca agccattacc
    19621 cgtgattaca cccttgatat ccatgatgaa aacggcaaag caccgctgct gtcggtattc
    19681 ggcggtaagc tgaccaccta ccgaaaactg gcagaacatg cgctggaaaa actgacgccg
    19741 tattatcagg gtattggccc ggcgtggacg aaagagagcg tgctgccggg tggtgccatt
    19801 gaaggcgacc gcgacgatta cgccgctcgc ctgcgccgcc gttacccgtt cctgactgaa
    19861 tcgctggcac gtcactacac gcgcacttac ggcagcaaca gcgagctgct gctcggcaat
    19921 gcgggaacgg taagcgatct cggggaagat ttcggtcatg agttctacga agcggaactg
    19981 aaatacctgg tggatcacga atgggtccgc cgcaccgacg atgccctgtg gcgtcgcaca
    20041 aaacaaggca tgtggctgaa tgcggatcaa caatctcgtg tgagtcagtg gctggtggaa
    20101 tatacgcagc agaagttgtc gctggcgtcg taaattaacg taaggtgatc aggtcagatt
    20161 tcaatctggc ctgagactga tgacaaacac aaaactgcct gatgcgctac gcttatcagg
    20221 cctacgtggt ttatgcaata tattgaattt gcatggtctt gtaggccaga taagacgttc
    20281 acgtcgcatc cggcatgaac aaagcgcact ttgtcaaaaa tctaacctac tttttaattc
    20341 agggaattac cgcaaagccc acgccatcat acccaacgta acaaaactca ggcacgttcc
    20401 ccactcgccc cgagaaaaat agcattaatg cgcccagcgt cagcataaaa atattgagca
    20461 gaaggtaaat tgttggcgta aagagtaaat tgcaaacctc aggaatagcg gcaagcccag
    20521 cccccagcat ggcaataacc gctgcaaaaa attgcgtcag tattgggatg ccgaaaaaag
    20581 tcattaccag cgaggtcaaa atccattttc tattttgcat tattctttcc attctttctg
    20641 aatggcgaaa ttatactccc cgagtcccct tgccccttct ggacactttt ccgaaatgat
    20701 ggcgaaaaaa acgggaccct ttgg
//
LOCUS       DAEEUE010000070        20714 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.070, whole genome shotgun sequence.
ACCESSION   DAEEUE010000070 DAEEUE010000000
VERSION     DAEEUE010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20714
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.070"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(77..4537)
                     /gene="mukB"
                     /locus_tag="KLK84_002729"
     CDS             complement(77..4537)
                     /gene="mukB"
                     /locus_tag="KLK84_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HBE2009995.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4537..5241)
                     /gene="mukE"
                     /locus_tag="KLK84_002730"
     CDS             complement(4537..5241)
                     /gene="mukE"
                     /locus_tag="KLK84_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HBE2009996.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5222..6544)
                     /gene="mukF"
                     /locus_tag="KLK84_002731"
     CDS             complement(5222..6544)
                     /gene="mukF"
                     /locus_tag="KLK84_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HBE2009997.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6541..7326)
                     /gene="cmoM"
                     /locus_tag="KLK84_002732"
     CDS             complement(6541..7326)
                     /gene="cmoM"
                     /locus_tag="KLK84_002732"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HBE2009998.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7462..8241
                     /gene="elyC"
                     /locus_tag="KLK84_002733"
     CDS             7462..8241
                     /gene="elyC"
                     /locus_tag="KLK84_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HBE2009999.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYEALGRIWQWLKGPSGEPRQE"
     gene            complement(8218..9111)
                     /locus_tag="KLK84_002734"
     CDS             complement(8218..9111)
                     /locus_tag="KLK84_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HBE2010000.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9265..10011)
                     /gene="kdsB"
                     /locus_tag="KLK84_002735"
     CDS             complement(9265..10011)
                     /gene="kdsB"
                     /locus_tag="KLK84_002735"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HBE2010001.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAEH
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10008..10190)
                     /gene="ycaR"
                     /locus_tag="KLK84_002736"
     CDS             complement(10008..10190)
                     /gene="ycaR"
                     /locus_tag="KLK84_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HBE2010002.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10242..11474)
                     /locus_tag="KLK84_002737"
     CDS             complement(10242..11474)
                     /locus_tag="KLK84_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HBE2010003.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11511..12497)
                     /gene="lpxK"
                     /locus_tag="KLK84_002738"
     CDS             complement(11511..12497)
                     /gene="lpxK"
                     /locus_tag="KLK84_002738"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HBE2010004.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12494..14242)
                     /gene="msbA"
                     /locus_tag="KLK84_002739"
     CDS             complement(12494..14242)
                     /gene="msbA"
                     /locus_tag="KLK84_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HBE2010005.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14279..16538)
                     /locus_tag="KLK84_002740"
                     /pseudo
     CDS             complement(14279..16538)
                     /locus_tag="KLK84_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16745..17029)
                     /gene="ihfB"
                     /locus_tag="KLK84_002741"
     CDS             complement(16745..17029)
                     /gene="ihfB"
                     /locus_tag="KLK84_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HBE2010006.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17189..18862)
                     /gene="rpsA"
                     /locus_tag="KLK84_002742"
     CDS             complement(17189..18862)
                     /gene="rpsA"
                     /locus_tag="KLK84_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBE2010007.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18973..19656)
                     /gene="cmk"
                     /locus_tag="KLK84_002743"
     CDS             complement(18973..19656)
                     /gene="cmk"
                     /locus_tag="KLK84_002743"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBE2010008.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19833..20594)
                     /gene="ycaL"
                     /locus_tag="KLK84_002744"
     CDS             complement(19833..20594)
                     /gene="ycaL"
                     /locus_tag="KLK84_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HBE2010009.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASG"
BASE COUNT         4982 a         5725 c         5033 g         4974 t
ORIGIN      
        1 ccgttatgtg ccattttttt agtgcagaaa cggagttttc ggaaaaagaa aaggcggcat
       61 tgctgccgcc ttaattttaa ctcgcctggg aaggcgcttc gtctcttcct agaagcgttt
      121 cagggagttg cggcgcaaat cctcgcaggc cgacgacgtg aacatgttcg gtattctgga
      181 agactttacg taccagttta taggtggtgc ctttttccgg actgatattt tccggcgctg
      241 cgatgatgag ctgcatttgc aaacgttcac acaattcaaa cagcgtggcg atagaacgag
      301 catccagtcg cgctgcttca tcgaggaaca gcaggcggca aggagagata tctttaccgc
      361 gcaggcggcg agattcatct tcccagcttt gtaccaccat caccagaatc gacataccgg
      421 taccaatcgc ctcaccggtc gacaatgcac cagactctgc gcgcaaccaa ccatcggaac
      481 cacggttaac ctcaacttcc atttccagat agttgcggta atccagcagt tcttcgccaa
      541 tggtctgcgg cgtgcgctgc cccatatcaa tctgcggatt aagacgctga tacaatttcg
      601 ccaacgcttc cgagaaggtc agacggttgc tgttaaacag atcctgatgc tgctcgtgct
      661 gttcagagag cacatccagc agcatggcgt gcgtttcacg cacgttcacg ttgagacgca
      721 cgctgttcac ctgaccaaac gatacgttct gcaacccctg gttgagcata cggatacggt
      781 tctgctcgcg ctgaatggtt ttgcgaatga tgttcgccac gctgcgggaa ctgatcgcca
      841 gtttctgttc acgggaggtt aattcttcgg tcagacggct aagttcaatc tccatctgtt
      901 cgatagcttc caccggatca tcggtacgaa taatatcctg acgaatacgt tcacgcagat
      961 gctgataaac cgccacgaag aactgaattt tacgctccgg acgtttcgga tcttccgaca
     1021 tgcgcagcac gtcgcgcaga tgttcgttat ccgccaccgc cagacgcagc gcacctaacg
     1081 ccttatccga catggaacgc aaatcatcag cggagagata agccagctca cgacggtgta
     1141 agcggcgctc aacgccgtta tctttcacca tgcgcatcac cgcacaccag cccgctttgg
     1201 cggtcactac ctgctcgcgc atctcaaagt aatcccgctc cagcttgcgc agtttgcggg
     1261 tcaggttgtc catctccgct tcgcagaagg taagcgcttt ttccagttga ttgcggcgtg
     1321 aacggttatt gctcagttgc gcatgcagct cgtcacggcg aatacgcgcc cgctcttctg
     1381 ccccgctatc agcacgcacg ccgatatcct gcaattcacg ttgcagatcg ttgagtagct
     1441 cttttttggt gtcgtaagaa cttttcagcg aagccagcac ctggttgtac tgactcaact
     1501 gcgctgcgtg accgcgcaac gcttcgcgag cgcgggtacg ctccgcttcc gcctgttcca
     1561 gacgttcacg cagtttttcg ttgagatcgc tgttaccgct aagcatttct gccgagtcag
     1621 aatagctaaa gtgcgcacga cgctgcacca cttccgtcag ggcaaacgct tgctgacggg
     1681 catcgcgctg catctgctga gagtacgcgt aatcttcttt taactgttcg aactgttccg
     1741 ggtcgctctg caataccgaa acgatcggtt ccagtttcgc cagttgattg ccaaactgct
     1801 gaacaaaacg cgcggcttcc tgggcttcat ccagacgttc gcggatttca tcgacgcgat
     1861 ccgccaggct gtcatcagcc aacaggttga gacgcggcag aatgcggttc agcgccgtaa
     1921 cgccttcttt cgcctgctca aactgaatac gctgctgctg gttatcattt tcatgattac
     1981 tcaacgcccg ctccagttcg actcgacggc tgttcagttg acggatttct gcttccgggt
     2041 cagactcaaa cgccaccgcc agatgactgc cgataaagcg gctgaacgcc tgatgcagac
     2101 gctgggtttt ctgtacatca aaggagagcg tggcgaagcg ttcggaaagc acttcacgct
     2161 cggcatggag gctttcaata cggctttcac gcgcagcacg accaaacagc ggcacttccg
     2221 ggaaacgtga ataacgccac tgtcgatcgg cgattttcac caccaccgct ttttccagct
     2281 catcaacgct gaacacgcta tcatcgaatg actgcggatc cccttcgatc aaatagagat
     2341 cttccgggca atcggtcaag ccttccaggt gttcggttac ctgtgacaga tctggcacca
     2401 cgatggcgtg gcgtgacggg ccatacagag ccgagaagta cggcgcatct tccaggctaa
     2461 cgtcgtcata aatttctgac agcagcacac caccaaaacg ctccgccagc gcgttcagac
     2521 gctgatcttc agagccgcca ggctggctta aacgttcgat ctcttcatcg acggcgtttt
     2581 tgcgcgcgcc tacttcatcg cgttcaacaa tcgcctcgcg ctcacgctcc agcaactgtt
     2641 gcagatattc agtgacgtcc tggctggagg agaactcttc gccgcactgt tcactcaact
     2701 ggttgagact gttttgcgct gccagccaaa ccggtgcacg ctgcatcaaa ctctgaatgc
     2761 gagactgcag ctgttcctgt tcctgccgca gtgccatccg ctcttcacgg gcgttagata
     2821 cgctatcgga aagagaggca atgcgtgctt ccagctcctg atgcagagct tccagttcat
     2881 caatatcaaa attcttgccc tgacgtttgc agaaatctgc cagcagacgc tcagcttctt
     2941 gctgctcacg cagacgctgt tccagttcgc ttaagcgcat ccgcaacggc tgaacctgct
     3001 ctgccaggtg acgctgatca accccttcgc gcaatagttc gcgagcgaca tcccacgcct
     3061 cgttacgcgc cagcgggccg ttgattgcca ccaccagctg ataagcctgc tcaaactggc
     3121 tgtgcgcggt ttgcgccatg ctcattttct gctcaagaga gagcattttt tcagtcgctt
     3181 ccagctcttt cgcctggaag gtttccagcc attcggcggc gctgtcggcg gttaagtccg
     3241 gcagatggca cagttctttg gcacgattaa gcgcagcaat cgcctggtta tactggatcg
     3301 cgcgcgtttg ctggacgtcc agcgcctgct ggtagtcagc aagctggctt ttcagctcgt
     3361 ccacttccag ttcggcagct tccgcgcgag cctcattctc ttcctggcgt tcgatggctt
     3421 ctgccaccac ttcattttgc tcttccagac ggatctgcag ctcatcgaga tccacttcgt
     3481 agcgttcgat cttctcctgc tgacgcaatg cggtttgcac caggttcagg tgatcgctgg
     3541 ccgcctgata atccgcttcc agatcgcctt cggcaccgtt gtgctctgcc agctcacgcg
     3601 ccatatcgac gtgtttgtac tgctcagccg ccagttgctg acgcgaagta tgtagctcgc
     3661 gacgaaactc cagggctttg tcgagatgga cacggcgctc gttggcgtga cgcatgtagt
     3721 ccgccgccac gtagttggta gcttcgctga tcagatgctt aaacaggtca cggtccgact
     3781 gggtgacacg aatcgcttcc agcgtcatgc ggttttcacg cagcgccgct tccatgtcct
     3841 ggaacgcttt acgcacgccg ctgttttccg gcaacaggta gtcgcgcaga gaacgggtaa
     3901 tggcactgga gatcccgcca tacagcgaag cttcgatcag acgatagaat ttgctacggt
     3961 cagatgcgga gcgcagacga cgcgcgatga tgcccagatc gaacatcagc gagtggtaat
     4021 cggtaatgga gttgaactgt ttaaactgca cgccttccat cgcctcgagc ttgtctttca
     4081 gctcgttaag cggcagcacg cgcgcctggc gttcgttcag ggtttcggtc accagctgtg
     4141 tcggctgcac cgacatcggc agtccctgaa tggcaaatgg tttgatatcc actttacgat
     4201 cgcgtccggc aacctgttgc agacgcacac cgaccaccac gcgctggtgg cgcgagttaa
     4261 tggtgtcgag catcgaataa cagacacccg ctttcagctt accgtgcaga cctttatcgc
     4321 gcgaaccgct ggtggccccg gcttccgtag tgttacggaa atgcagcagg gtcaggtcgg
     4381 ggatcagcgc cgtaacgaac gccgccatcg tggtggattt acccgccccg ttaccgccgg
     4441 aaagcgtcgt gaccagctcg tcaaggtcaa aagttcgggc aaaaaagccg ttccagttaa
     4501 tcagcgtcag tgagcgaaat ttaccgcgtt caatcattat tcttcctctc cgctatctgg
     4561 ctgactctct tcggtttcat cgttgagttg cagatgattt tcaatcggca ttgcttcgcc
     4621 atcacgaatc aggcgacgct gtgcttcacg gggatcgtcg ccagcacgca catcggctcc
     4681 gaagcggaac accgattcgg taatgcggaa cttgctgctg tcgtggccca taaaccacac
     4741 catgcctaaa cgacgcagac ggttgagcga agaacgtact ttctcctgca acttctgacg
     4801 gtcaacgtct gaaccggttg aacggttgtt caccagtttc agcagtttcg cttcatcggc
     4861 cagggtgagc agttcgtcgt acagttcctg ctgggtgaaa atcccctcat tcgccagccg
     4921 ttccgggctg agatagagat aacagaggat tttcccgacc atcatatcca gttccgacaa
     4981 gacggaacgc gggataagag tggtggaacg tgggcgtaaa tagaagaacc cttccggtgc
     5041 gcgaataagc tcaacgttat aacgcgcgta aaactcttcc agatattcct gaaaatccat
     5101 caggaatgca tgattatcca gttcgtcgag gccaatatgg cggcctgaac gtaaggcgct
     5161 gtccagcgcc ggaaataacg gattcgccag cgcctgcgcc agcttaaccg gcatcacttg
     5221 ttcaatattt gtcgatgaca tgcgcctgta ccttggctcc gtaatcatta atcggctgcc
     5281 atttcgctgg cagtccggtg aaatctgctt gcgctacgcc aagacgtacc gcctgatcaa
     5341 taacaatacg cgcaacgtca aagtgacgtg cacgcggata ctgtgacaga tattcgcgta
     5401 ccaccagacc aagatccagc ggcacttgtc tggttttgta cacggcaagt tgttcttcga
     5461 tgatcgccgc cagctgttcg cggatctcgt taaactcttc gtattccaga tcctcaggaa
     5521 gttccccagt cacttcttca tcgcgcagtg ccatctcttc gtcacgcata tccagcagac
     5581 gatcggcatt ggcataagtt agcgcccacg gctcatcaaa ataggtttgt accgactgac
     5641 gtaaccgctg agcaaagacg cggtttttat ccatatcgat cgcggtacga ataaatttgt
     5701 gtacgtggcg gtcgtagcca atccacaagt cgatggattg ctggccccaa ctgataatac
     5761 gatcgagttt gctctgcaga tcgaacacca gacgatcgac gaaatgcaga tcgtcatggg
     5821 tcatcgtcgc atcctgaatg cgcaacagat tagcctgcaa tttgtcgcct gccgcttcca
     5881 gcgtatcctg caattcacgc agcgttccgg aagtttccga aagcaacaat tcacagctgg
     5941 aaatcgccgc ccgccagtct ttgttcagca actgggcgat atcgtccttc acctgctgct
     6001 gctgttcgtc catcagacgt tgcgtcaggt cgatactgtc gaaaatttct gctaccgaat
     6061 atttcagtgg cgcatagaca ttacggtgcc agtgaaattc atcaccgccc tcttcggcgg
     6121 catctgctgc gcgtttgagc tcacccgcca caatcgacaa ctgcatagaa agacgcagcg
     6181 tagaaaactc gcgctgacgg atgtagtagt cagtaatgcc gatgccgagc ggcgtcagac
     6241 ggtaaattgc gttcccttcc gcctgctcgc tggtaaagcg gttcagcaga cgttgacgca
     6301 ccatgtcgtt gatcgcgtta ttggcgcgca cgccgatggt ttcgctggtt tgctcaaacg
     6361 catcactcac atggcggaat gcatccacca gctcgccttc actcatctca ccatccagac
     6421 gctcgccgtt cagcgtggca accgccagca gaaaagagag tcggtctacc ggcagcgaga
     6481 tggagaagtc attttttctg gcccaggcaa ccagttcggg gactgtctgg gaaaattcac
     6541 tcatacttta tccttgctct gcggtttgcg cgcggtgaca tgaatataac gccctagggt
     6601 aatatacggt tcctgacggc aataacgcgt ttctaattca agtaatgctt catagcagtc
     6661 gcgctgctgg tacttctcgc gcagataatc atgaaacacg cgaacgcctg tcttacccat
     6721 aatttgccaa ccagcttctt ccagccacag ataaacctgc gccgggtcgc gtggataatc
     6781 tggcgaaagc gtccgttttt tctttttcgg cattcccgcc tgcacgtaat caaaattccc
     6841 ggcgaccatg ttatgcatca acaaaccatg cgcattgtag aacattaacg acaacactcc
     6901 gcctggacgc aacactgacc acagggtctg caatacactg cggggatcgg ccacccactc
     6961 gagcaccgca tggaacaata tcagatcaac gggcgtttcc aaatgcgaag caacatcctg
     7021 agcggcgcaa tgtataaatt gcatgttgtc gctcacacct tttgcttctg ccgcctgttt
     7081 tgcgcggtcg atcatctgcg cagaaagatc gcataaaata acctgatgcc cgcgctcggc
     7141 cattttgatt gcggtctgcc cttctccgcc gccagcatcc agcacacgca gtttttgcgg
     7201 gcccatttcc gccagcacgc gatcgagatc ctgccacaga atagcctgtc gaagctgccc
     7261 tttggtggtg ccgtaaatgt tacgggaaaa cttttccgca atatcatcaa aattgcgatc
     7321 ctgcattgct ttctccaccg gttggctaag acgttttctg ataaagacgc tattttgtca
     7381 cagccacggc gagaatgaac ccgtctggtg taatatcgct agcgatccct tgctatatgg
     7441 ttaaaaaagg aaccagaaag gatgcttttt acactgaaaa aagtgattgg caatatgttg
     7501 ctgccacttc cgctgatgtt gttaattatt ggcgctggcc tggcgctgct gtggtttagc
     7561 cgttttcaaa aaaccgggaa gatttttatt agcattgggt ggctggcact cttgttattg
     7621 agtttgcaac cggtagccga ccgcctgctg cgtcccatcg aaagcaccta tccgacatgg
     7681 aataattcgc aaaaagtgga ctatatcgtg gtgctcggag gtggttatac ctggaacccg
     7741 cagtgggcac cgagctctaa tttaatcaat aacagtctgc cacgcctgaa tgagggtatc
     7801 cgtttatggc gcgaaaatcc gggttcaaaa ctgatcttca cgggaggcgt agcaaaaacc
     7861 aatacggtga gtacagcgga agtaggtgcc agagttgcgc aatcgctggg cgtaccgcgc
     7921 gagcaaatta tcaccctgga tttgccaaaa gataccgaag aagaagctgc ggcagtgaaa
     7981 caggcgattg gtgacgcccc tttcctgctg gtgacctccg catcacatct gccgcgggcg
     8041 atgatctttt ttcagcagga agggttaaat ccgctaccag ccccggcgaa tcagttggcc
     8101 atcgactcgc cgcttaatcc gtgggaacgg gcaatccctt ctccggtctg gctgatgcat
     8161 agcgatcgtg ttggctatga agcgcttggc cgcatctggc agtggctgaa aggtccgtca
     8221 ggcgagccac ggcaagagtg attttgatgc cagatcgaag ttgcgccgac taaatcgtcc
     8281 ggtattaacc agttgcgcaa cttcatccca taacacatac aaccaacgcc gccagatgaa
     8341 cgactccgcc actggcgcgc gttgcaggta actccagagc aaatcttccg ccagagaatt
     8401 atccattagt cggaacagtt cgtattctcg cggtgcccaa agcattagtc ccggcccgac
     8461 catcgccagt aactgatcgc tgcgcgaatc tttcagcatg ctgcgtaaac agaagttacc
     8521 gtgaatcaac acacaattgt cgttaaagcc ttcaaataat gccgggagac attcgcgagt
     8581 gcgaaacagg atccgcttat cctgcatcgt cagaccggtg ttattgaact gattgagcgt
     8641 ggtccatagc acttcaacat gttgccggta ccatgagggc cagaaatttt cctgagtatt
     8701 gtcgaccgcg ccgacgcaac cgcgactgtc ctgacggtgc caggccagta aggcttcaac
     8761 gatttggtct ttgagctgtt cccagcgttc tggtgttcgg gctggtgcct ccaccgaaac
     8821 accacgcatc cgctccagta gcaggacatc agggccgggg tgttcttcat gcgtcatcac
     8881 accataaata gtcggcatac ggacagtccc acttcttgcc agcatggtgg ttttccatgc
     8941 cagttgtcgg gcttttccgg gcgtactaaa gcttcttgcc attaacggca ttgggtttcc
     9001 ctggctgtca tacaaagccc acaacgccgt atccgctttt tcattgacgc actcaatacg
     9061 actgagtttt tcgcccagta aatggcttaa ttcggcacgc agctgttcca ttcagattac
     9121 cctcataaaa gctactgctt tcataatgag cgccaatata aaagatgtca cctgaatgag
     9181 atcaattgtt gaaaagaaaa attgaggaaa atcgtaacgg tacgacactc ctcccaaaat
     9241 tggctgaagt gtcgtgaagt gaaattagcg catttcagcg cgaacgcgct caagatcttc
     9301 aggggtatcc acacctgtgc caggaacttc ctgagcaaca gcaacatgga ttttttcgcc
     9361 gtaccacaga acacgaagct gctctaacat ttcgatgtgt tctaacggac ttgcctgcca
     9421 gttgacgtaa cgacggataa agcctgcacg gtagccataa ataccaagat gacgcaggaa
     9481 gttatcgcca acggtttcaa ggccttctgc aaaacgatca cgatcccaag gaatggtggc
     9541 acgtgagaag tacagtgcat acccttcagc gtcgagaacc actttcaccg cattcgggtt
     9601 aaacgcttct tccgcattgt ggattggcac cgccagagtc gccataccca cctgacgctg
     9661 agcgaggtta tcagcaacct gacgaatgat tgtcgcaggg atcatcggtt catcaccctg
     9721 cacattaacg atcaccgtgt cgtcgctgaa tgcgcatttt tcgacaactt ccgccagacg
     9781 ttctgttcct gactgatgat cggcgcgcgt catacatact tcaccgccag cggcttcaac
     9841 ggcgcgggca acatcatcat gatcggttgc cacgatgatg tgctcggcac ctgattcacg
     9901 cgcgcgttca agaacatgaa caatcatggg tttgccgtta atatcaacca atggtttacc
     9961 gggcagacgc gtggacgcgt agcgcgcggg aataatgacc acaaaactca tgatttactc
    10021 tcatcagcag tcagcacgcg ggcttccgtt tccagcaaca ctggaatgcc atcgcggagc
    10081 gggaaagcca ggttatccag tttgcaaata agctcttgtt tttcctggtt ataccaaagt
    10141 tttccgttgc aaaccgggca ggcaatgatt tcaagcaaac gatgatccat agttcctcca
    10201 gatggatcgg gttatgaatg cataaatctt atcataatca tttatgcgac ggggtctatt
    10261 tcccagccag tgcggcaatc agtaaagaga ccttgcgggc agcgtcctaa tgtcacccgc
    10321 gttgcctgct gccagctcgc gaaatcagta atcgcctgac gtaacccttt ttgcagcatt
    10381 gtcgttggtt taatgccttc ctgtaaccac agagagataa cttcaaggat gcctgtctgg
    10441 cgatgcattt tggcatccat tcgcccaact aattgcccac gatgtaataa tggcagaaca
    10501 aaatagccat actggcgttt cggcgctggg gtatagcact ccagccggta gctaaaatca
    10561 aaaagctgct ctgcgcgttt gcgatcccag acaacaggat caaaaggcga aagtaccgcg
    10621 ctgtgcgttg cagtgagctt tccggccagc gcacgctcga gtagcggcag caaatcagca
    10681 tgcagccaaa gattgcccaa tttttcaaca tgcacagcaa tgatttgctg ctgttcagcc
    10741 ctcgcttcgc gccacgccgc cagtgccgga cgtttcagcc gatagtaatc tgccagccac
    10801 tgttcgcgga atattcccag gctacgcgca ctgttatcca gcatgatgat ttctgcttct
    10861 gtttgcgaaa cgagatcgcg ctcatcatcc cagtcaggca tgacacggtg ggttaaatca
    10921 taaacgcgct ggaagttgcg ccgttcaatc accatcacct ttccggcagt aaataaacct
    10981 tccagatgcc gtttatgcgg cttccattcc caccagccgc ttgcaccttt acgaggatgc
    11041 tcaaaatcgg ctgaacgtac cggcccttta tcatgaatat gctgaattaa ctgtgcaatt
    11101 tccgcctcat gttcctgcat ccaggcgtct ttgtatttcc agcccatttt ttcaggtgcc
    11161 agcatgcggt ggcgaataag acgaaagtcg ctacgcggca taaagcaggc ttcatgcgcc
    11221 cagtattcca ttaattcgcc acgcgccaga gactcatcca gccactgggc aggataattt
    11281 cccagacgac tgaaaagcac cagatatgga ctacgggcaa caatattgat ggtatcgatt
    11341 tgcagcaagg acatgcggga gatcgttgcc ggaatatcct ccaacgacgc tcgacggcgg
    11401 ggtttgttta acaggccttg tgcggcaagg tgaagattac gcgcatcagc aagggagagg
    11461 tgcggcagcg acattcatga ctccatcaat cgaacgctgc cgcggcgtaa ctagttgcca
    11521 gaagccagcg aggttagttg cgtaagcagt ttcgctggtt catcacctga aagctgtgcg
    11581 tctacaggca aataccacca attttcttct gcaaaggccc ggcatttcac cgcatctttt
    11641 tcagtcatta ccagcgtttg cccggcgctt accaacgcac tgacatccgc atggttcaaa
    11701 gactgatgat cggccagcgg tacacatttt tccggttgta cgccacacat cttcagcgtg
    11761 gcaaaaaagc gcggcggatg cccaatcccc gccatcgcca ctacatgttc aagctgagca
    11821 acgtcacaac gcgtaccggt acgtaaattc accgcctgac ccggcagcag atgcatgggg
    11881 atttcaccgc tgcgagggac accgccgttg acgattaccg catcaaccga ctttaagcgc
    11941 cccgctcgct cacgcattgg ccccgccggc aaccaccagc cattgccaaa gcgacgcaca
    12001 ccatcaataa cgacaatttc cacatcacgc gccagacggt aatgctgtaa accgtcgtcg
    12061 gttacgatga tctgcacatc agggtgttgc gccagaatgg cttttaccgc atcagaacga
    12121 acgggagaaa ccgcaacagg cgcatcagtg cgttgataaa tcaacacagg ttcatcaccc
    12181 gcctgtgctg tggtggtatc tgccgacaat aacagcggat aagattcagc cttaccacca
    12241 tatccccgcg atacgacccc cacgcgaata ccgcgctgtt gcaactgttc caccagccag
    12301 acaacgaccg gggtttttcc gttgccgcct gcggtgagat taccaaccac gacaaccggt
    12361 acgggggcac gccaggcgcg cttcagtttt agtttatagc aaagacggat cgcgccactc
    12421 accaggccat acaaccagga gagtggcagc aatagccgcc acaaagggga ttcaccagac
    12481 cagatttttt cgatcattgg ccaaactgca ttttgtgaag ttgcgcgtaa acgccgcggt
    12541 gctcaagcaa atcgttatgc gtaccgcgtt ccacaatgac accatcctcg acgaccacga
    12601 tttcgtctgc cttttcaatg gtagacaagc ggtgggcaat caccagagag gtacggtttt
    12661 tctgcaactc atccagtgcc gcctgaatcg cacgttcgga ttcggtatcc agagccgagg
    12721 tagcttcatc cagaatcaga atcgggctat cacgcagcaa ggctcgagcg atagcaatac
    12781 gctgacgctg accgccagag agcagcacgc cgttttcacc aatcactgta tcgagaccgt
    12841 tatccatctt attgatgaag tccatggcgt aggccatccg cgccgcttct tcaatttgct
    12901 cacggctgta ctgttcagtc cgtgcgtaag caatgttgtt agcaaccgta tcgttaaaca
    12961 gatggacatt ctgcgacacc agagcaacct ggttacgtaa cgacgccagg gtatactcgc
    13021 gcagatcgtg accatccatc aggatttcgc cttcatcaat atcgtaaaaa cgcgtgatca
    13081 ggctggcgat ggttgattta cctgaaccag agcgtccaac cagagcaacc gtcttccctg
    13141 ccggaatttt caggttgatg ttacgcaatg cgggtacgtc acgtcccgga taagtaaagg
    13201 tgacattgcg gaattccacg tcgccagtcg cacgctcgat cacgcgctta ccttcgtctt
    13261 tctcctgctc actgtccaga atggtaaaca gcgtctgaca agccgccata ccgcgctgga
    13321 actgggcgtt aacgttggtc agcgatttca gcggacgcat cagtgcaatc attgaagaga
    13381 aaacaacggt aatcgtaccg gcagtcaggt tatccataac acttgggaag ctcgccgcat
    13441 acagaacaaa cgccagcgcc aaagaggcga tcagctgaat gatcggatca gagatggaag
    13501 aggctgaaac cattttcatc ccctgaagac gcattcggtt actgacttta tcgaagcgtt
    13561 tcgtttccac ttcctgacca ccgaaaatca atacttcttt gtggcccttc agcatttgtt
    13621 ctgcactggt ggtcacttgc cccatggtgt tctgcatgtt tttactgatg ttgcgaaaac
    13681 gcttcgatac tacgcgaatc gcaatcgaaa caatcggtgc cagcacaatc aaaatgatcg
    13741 acagttgcca actgtaatag aacatcatga tgaacaggcc gatgatcgac gcaccttcac
    13801 gcacaacagt aatcagtgcg ccggaagaag aagaagcaac ctgttcggaa tcgtaggtga
    13861 tacgtgacaa caacgtaccc gttgactgtt tgtcaaagaa tgaaactggc attcccatca
    13921 tgtgaccaaa caggcggcga cgcatggtca ttaccacctt tcctgatacc caggagatac
    13981 agtagctgga gacatagctg gtgataccac gtaaaatcat cagtccgatc accaccagcg
    14041 gcatccacac cagcacggag cgatctgttt taccaaagcc atcatcaaga agtggcttaa
    14101 ggagcgataa catgaaggta tcgctggctg cgttgaggat taacgctacg cccgccacga
    14161 tcagacccgc tttgaaaggc gcaatggttg gccacagtcg gcggaatgtc tgccacgtag
    14221 agagatcttt gtcgttatgc attcaaaaaa ccagcatttg ttgaaatagc cgcatattct
    14281 acccgttatc cactggcacg ccaaaccact gatgatacca acgaggtaaa atttgctctc
    14341 tgaggctgct aatccgccag ccttgtgctg aaaaattgac cgtcacttga ccctgatgtg
    14401 gagtatcaag ccattgatat cctttctgtt gatagcgatg cttaactttg ttagagggca
    14461 atcgccatgc gttatagcgc gatgccgatg cgagtgccac ttttccattc actcgctgaa
    14521 ttaatggcaa cgatgatgag gtattactgc catggtgagg tacctgaagc aatgttgcct
    14581 gcacttgctg ccagtaacgg cttagcatct tttgttcagc tggggcttca atatcaccgg
    14641 ttagaagaat gctattcgtc ccgtcatcaa ccttaaccac acaggaatgg ttatttccct
    14701 tatcgttgct accttgtaaa ggccagtgcg cgctgaaacg caatccttgc cactgccacg
    14761 cttcgccacg cacacagggc tgatgatgtt cccagtttaa cggacttctg atccataaca
    14821 tcggccatat atgcaatatt gaatccagcc ctccccggtg atccagatgt tcatggctca
    14881 gaataacgcc ttccggttca agattatgcc agtggagcca ggggataatc agttgttgcc
    14941 cactatcccc ttcaggccag tcctgtgtca tagagaatcg ctttgccgtt tctggctatc
    15001 accattgcca gcccttgccc gacatcaagc atgtacagct gccactcgtc aggtcgaggt
    15061 ttttgccaca gcggccagca catcagcaag cctccagcca cacacattgc tggcaacgtt
    15121 cgccaggcgt ttaatcgcca taccaccagt aagaaccatg gggaaaatga tagccattgc
    15181 caacattcaa caatgttgat ccacccttcc ggcaaactct ttaaccccca gaaaagtaaa
    15241 gccaaagacc ggtcggcaag aaaccataac ccctgttcta ggattaacgg cccgctaaaa
    15301 tgcacaacca tcgcggcgag gatcaacgga accgtgataa atgtcaccaa gggaattgca
    15361 aatagatttg caataaacga ggtcagacta atgccatgaa atatgacgat ttgcatgggc
    15421 ataagcagga gtgtgattcc cagttgcaga tggatgaggg aaacaactgc acgcaatacc
    15481 ggcggcagtt gccactcagg acagggaaac cactgatacc aaaaaatcag tgccgcgacc
    15541 gcagcggcag agagccataa actttgcgag agaatggcaa caggatccat cagcaaaatt
    15601 gccgccagac aacatatcca tacatcccag ccactccact gtcgcccact taacttaagc
    15661 attccccacg tagcaagcgc caccacggta cgcaatgcag gaggttgcat tcccgtcaac
    15721 caggcataaa aagcagcaca gcagattccg ccaattaatg gtatttgcca gtggatccag
    15781 cgcccaggca gaaaaatttg tccactgcga atgagtccgg cagccagcaa cgccgcaaaa
    15841 gcgatgtgca atcccgatat cgccattaaa tgcgccgttc cagtatcgcg catgatattt
    15901 ttgatttctt tggggacgga taaccgttcc cccataccta aaccaagaat aaccgcattc
    15961 cacggatagg gttgcagcgt tgtttgtagt gacgccagat actgtgcacg caggctacaa
    16021 ttcggttcaa tgacacttgc ctgcagaaaa cggccggtga gcggctgatg ctgggcaatg
    16081 gcataacgct ggctatcaaa gccgccatca ttaagttggc catgaactgc acgaactttg
    16141 agtttcattg accattgttg tccagcacaa accgcttgcg gaagttattc gccatacagc
    16201 acaaggcctg gcgcagggaa tatacgtcga ccttgtagat aagtaatttg accgtaatga
    16261 gtggtcatgc cgtcagttgc agtgatctca acaattgcat cctgcgtcgc gccagttaag
    16321 gtttctcctg cccacaaaat ttgctttgct gataatatgc cccaaacgaa aaacagcaac
    16381 gtcagcgcga tataacggac ggttttaaca gggataaatg ccagtacgca ggcgaagaga
    16441 gtcagaaacg caagggttaa tgtcctaggc aattggggca aaatcagcaa cggaaaaatt
    16501 ccgcaaatta tgcatacgcc gactgtcgtt attttcatgc acccctccct gtttatggaa
    16561 agtgtgcaac tttgttgagc ggttgtctgg tgggaatttt gcgttccccg aggcacattc
    16621 agaagaattt aacgaaaggg tgaaaactgc tcaaaactct gcgaggcagc ttccagttcc
    16681 tgattagaga attgaaaaaa gcacccgaca ggtgcttttc tcgcgttcaa gtttgagtaa
    16741 aaacttaacc gtaaatattg gcgcgatcgc gcagttcttt accaggttta aagtgaggaa
    16801 cgtattttcc ttccagttct actttatcgc cagtcttcgg attacgtccg gtacgtggtg
    16861 cgcggtagtg caaagagaaa ctgccgaaac cgcggatttc aatacgctcg ccctgcgcaa
    16921 gagtcgaggc catatgctcc agcatctctt ttactgcatc ttcaaccgtc ttggcgggaa
    16981 tgtgcgattg ctgggtggca agtctttcta tcaattctga cttggtcatg attcctccgg
    17041 ttccttaaag gcaaattaag cggctgctgt tgctgcattg attaagggcg gccgtagccg
    17101 cccttagtgc ttgattacag gacgaaacct gcaatctgtc aagtaaactc aacaaacttc
    17161 ggaataaaaa tcccgaagag tcagagaatt actcgccttt agctgctttg aaagcttcag
    17221 ccattgcgtt gttggagaag tttgcatctt cctgtttgtt aacagttgcg attgcatctt
    17281 tctcgtcagc ttcgtctttc gcacgaacag acaggctgat tgcgcggttt ttacgatcaa
    17341 cgccggtgaa tttagcttca acttcgtcgc caacgctcag aaccagggta gcgtcttcaa
    17401 cgcggtcacg ggatgcttca gaagcacgca ggtaaccttc aacgccgtca gccagttcta
    17461 cggttgcgcc tttagcgtca actgcagtta ctttaccggt tacgatagcg cctttcttgt
    17521 tcagagcaac ccagttgttg aacggatctt ctgcgagctg tttaacgccc agggagatac
    17581 gttcacgttc tgcgtcaacc tgcagaacaa ctgcagcgat ttcgtcgcct tttttgtatt
    17641 cacgaactgc ttcttcgcct gcaacgttcc aggagatgtc agacaggtga accaggccgt
    17701 cgatgccgcc gtccaggccg atgaagatac cgaagtcagt gatagacttg attttacctt
    17761 caacacggtc gcccttgttg tgggtttccg cgaactgctg ccacgggtta gctttgcact
    17821 gtttcagacc cagggagata cgacgacgtt cttcgtcgat atccagaacc ataacttcca
    17881 ctacatcgcc aacgttaaca actttggacg ggtggatgtt tttgttggtc caatccattt
    17941 cggaaacgtg taccagacct tcaacgcctt cttcgatttc aacgaagcag ccgtagtcag
    18001 tcaggttggt cacgcgacca gtcagtttgg taccttccgg ataacgttta gcgatagcta
    18061 cccacggatc ttcgcccagc tgtttcaggc ccagggatac acgggtacgt tcgcggtcga
    18121 acttcagcac tttaacagtg atttcgtcgc ccacgttgac gatttcgctc ggatgcttaa
    18181 cgcgtttcca ggccatgtca gtgatgtgca gcaggccgtc aacgccgccc agatcaacga
    18241 atgcaccgta gtcagtgagg ttcttaacga tacctttaac ttccatgcct tcctgcaggt
    18301 tttccagcag ctgatcgcgc tctgcgctgt tttcggattc gataacggca cgacgagaaa
    18361 caacaacgtt gttgcgcttc tgatccagct tgattacttt aaattcaagc tctttgcctt
    18421 ccaggtgcag agtgtcacgc accggacgaa cgtctaccag agaacctggc aggaacgcac
    18481 gaataccgtt cagctcaaca gtgaagccgc ccttaacttt gccgttgata acaccggtaa
    18541 cagtttcagc atcttcgtaa gctttttcca gcgtgatcca ggcttcgtga cgtttagctt
    18601 tctcacggga cagcagagtt tcaccgaagc cgtcttctac tgcgtccaga gcaacgtcaa
    18661 cttcgtcacc tacctggatt tccagctcgc cctgggcgtt tttgaactgc tcagccggga
    18721 tagcggactc agatttcaga ccagcgtcaa ccagtactac gtctttgtcg atagcaacaa
    18781 caacgccacg aacgatagaa cccgggcggg tttcgatttc ttttaaggac tcttcaaaga
    18841 gttgagcaaa agattcagtc atgtttaatc ttcaggttta tatttaacgt ccacctggct
    18901 ccatgccgga tggggttgtt taacataccc gctgtcattc cattgcaacg ggggtactgc
    18961 aaattcggtc gcttatgcga gagccaattt ctggcgcgcg tattgtagcg ctttttcaat
    19021 caattgctca atgcttaagg tggtggaatc caacactaaa gcatcggctg ccggaaccag
    19081 tggcgctacc gctcggttac gatcgcggtc gtcgcgttct ttgatctcgg ccaaaaggcg
    19141 ctcgaagtta acactaaagc ccttctcctg caactgtagc atgcggcgat gcgcacgttc
    19201 ttccgaggag gcgtcaagga aaattttcac tggtgcatca gggaatacca ccgttcccat
    19261 gtcgcggcca tcggcaatca gacctggtaa ttcgcgaaac gcgcgttggc gacgcaataa
    19321 tgcttcacga acgcgtggga atgccgcgac ttgtgaagcc gcattcgcca cttcctgagt
    19381 acgaatttcg ccgctgacat cttccccttc gaggatcact tccagattgc cattggtcga
    19441 cacaaaacgt acatccagat gggatgccag cggtaccagc gcatcttccg acgcaacatc
    19501 aacatggtga tgtaatgccg ccaatgccag tacgcgataa attgcacccg agtccagcag
    19561 atgccattgc aacgcttccg ccatagcctt acacaaggtg cctttccctg caccgcttgg
    19621 gccatcaatg gtaataaccg gggcaattgc cgtcatcttt atctccttaa aacaggcgta
    19681 ccgttaacat aaccgcgcgc attatacgcg ccgacgccac aaatcgttaa cgttgtgtgc
    19741 aaaaaccagt tttgtctgta ttcagatggg gatttattgc gcaattatag cgggctggca
    19801 gaagaccagc ccgaaagatg acaatgattt acttagccag aggcgatacg atcacggatg
    19861 tgttgcgcac gctctgttga tggtgggtga gagtcaaaca tggactgggt gcgaccgcca
    19921 tccaggctag ccagtttttc aaagctgcca accagcccct gggtgctgat gccacgtttc
    19981 ttcaacagat caaaagagaa atcatctgct tcggactctt tatcacggga gtatttagca
    20041 ttaatagcgc cttctgcgat atcacccaat tgtgagcggg aaagctggga agccacaccg
    20101 ctggtagctg aaatggcatc gcgtgcggca acgatcgcat aagaagcttt catttcagcc
    20161 agcgagtgac ccaacgcgac gtggcccagt tcatggccca gaacaccttc aatttcgttg
    20221 tcattcatca tgtccatcag gccactgtag acacgaacac agccgttcgc catcgcccat
    20281 gcgttgacgt cgctggtcat ataaacctta tagttgaccg gcgtgccatt aatattgtta
    20341 cccaatgctt tggcgatttt tgccagacgt ttaccgtact tgctggattt acttgcgact
    20401 tgattgccgc tgtccatttc ggcacagccc tgattggcaa tcgcttttgc atcggcatcg
    20461 cttaatgttg ctgctttata ggcatttaaa ccggagctga tagccatatt ggtatcaata
    20521 ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg tcgcaatcgc cagcagtaat
    20581 ttagtattct tcatgtgaat catccgttga ttaatcatat ataaatatta tgtatcaaca
    20641 acttacattg acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc cgaaaaaaaa
    20701 cgccgacaat gtgc
//
LOCUS       DAEEUE010000071        20521 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.071, whole genome shotgun sequence.
ACCESSION   DAEEUE010000071 DAEEUE010000000
VERSION     DAEEUE010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20521
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.071"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="KLK84_002745"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="KLK84_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBE2010010.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    687..812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="KLK84_002746"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="KLK84_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HBE2010011.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="KLK84_002747"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="KLK84_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HBE2010012.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="KLK84_002748"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="KLK84_002748"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HBE2010013.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIHVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="KLK84_002749"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="KLK84_002749"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HBE2010014.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..4814
                     /locus_tag="KLK84_002750"
     CDS             4449..4814
                     /locus_tag="KLK84_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HBE2010015.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4964..5482
                     /locus_tag="KLK84_002751"
     CDS             4964..5482
                     /locus_tag="KLK84_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HBE2010016.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5472..6697
                     /gene="dgcN"
                     /locus_tag="KLK84_002752"
                     /pseudo
     CDS             5472..6697
                     /gene="dgcN"
                     /locus_tag="KLK84_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6713..7195
                     /locus_tag="KLK84_002753"
     CDS             6713..7195
                     /locus_tag="KLK84_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBE2010017.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="KLK84_002754"
     CDS             complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="KLK84_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBE2010018.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="KLK84_002755"
     CDS             complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="KLK84_002755"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBE2010019.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="KLK84_002756"
     CDS             complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="KLK84_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBE2010020.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="KLK84_002757"
     CDS             complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="KLK84_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBE2010021.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="KLK84_002758"
     CDS             complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="KLK84_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBE2010022.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10939..11730
                     /locus_tag="KLK84_002759"
     CDS             10939..11730
                     /locus_tag="KLK84_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HBE2010023.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11751..13037
                     /locus_tag="KLK84_002760"
     CDS             11751..13037
                     /locus_tag="KLK84_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBE2010024.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="KLK84_002761"
     CDS             complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="KLK84_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBE2010025.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13808..14686
                     /gene="nadK"
                     /locus_tag="KLK84_002762"
     CDS             13808..14686
                     /gene="nadK"
                     /locus_tag="KLK84_002762"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HBE2010026.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14772..16433
                     /gene="recN"
                     /locus_tag="KLK84_002763"
     CDS             14772..16433
                     /gene="recN"
                     /locus_tag="KLK84_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBE2010027.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16582..16923
                     /gene="bamE"
                     /locus_tag="KLK84_002764"
     CDS             16582..16923
                     /gene="bamE"
                     /locus_tag="KLK84_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HBE2010028.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16985..17275)
                     /locus_tag="KLK84_002765"
     CDS             complement(16985..17275)
                     /locus_tag="KLK84_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HBE2010029.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="KLK84_002766"
     CDS             complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="KLK84_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HBE2010030.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17873..18355
                     /gene="smpB"
                     /locus_tag="KLK84_002767"
     CDS             17873..18355
                     /gene="smpB"
                     /locus_tag="KLK84_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBE2010031.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18570..18932
                     /gene="ssrA"
                     /locus_tag="KLK84_002768"
     tmRNA           18570..18932
                     /gene="ssrA"
                     /locus_tag="KLK84_002768"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19137..20378
                     /locus_tag="KLK84_002769"
     CDS             19137..20378
                     /locus_tag="KLK84_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBE2010032.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIEPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5302 a         5358 c         4815 g         5046 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctagaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggctaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacatgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt caccttgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 accatctttg atttgagcaa ccacagattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgctcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acagacaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acattccctt taaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta
     4561 tggaagatgc gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttaccta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaagggc tgatgaaaat gccagcacaa
     4801 tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt
     4861 tttatttctc acattgatga cggtcgcatg aaaaaatgat attttaatga gcatcctgaa
     4921 aaaatatcga tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg
     4981 actgttctta cttctgattc tcctgctgac gggagcacca atactcgctc aagagccttc
     5041 tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc
     5101 tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct
     5161 acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga
     5221 ggcgatgatt tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat
     5281 ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga
     5341 gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt
     5401 aaacctggat gcctcatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc
     5461 acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag
     5521 cattacgcaa catcagtatg accagcatat ttatcactat gatgctgatc tggttgctgc
     5581 tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaacctg gcactgacag
     5641 cagcaacaat gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa
     5701 ctgaaacact ggcagcgctg ggccagcaag ggcattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcat cctggcatta cccccgtaag gatccaggcg atactttcag
     5821 caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg
     5881 tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaatcagcc attttatctg
     5941 gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct
     6001 cacccgccat ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca
     6061 tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta tcgctgagtt
     6121 tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg
     6181 tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct
     6241 ggctaaccgc gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg
     6301 aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg
     6361 gggtcatgcg acgggcgata gggtcttgat tgaaatcgca aaacggttag ctgaatttgg
     6421 cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatga
     6481 tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct
     6541 cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat
     6601 gaccattgag cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca
     6661 ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa
     6721 gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca
     6781 gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc
     6841 cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt
     6901 gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct
     6961 aacacatgcc cgtatggatg gacacaccga taactatggt gaagacagtt acaacgaagg
     7021 cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctattggtg gacaaattcc
     7081 acgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac
     7141 cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attacaaccc cttaaaacac
     7201 gagcgaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct
     7261 taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc
     7321 aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact
     7381 accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca
     7441 gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc
     7501 agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg
     7561 aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata
     7621 atttaatctc ttatcctggg taaactgatc tctcgggggc ttacgccatc ccatcatgtt
     7681 tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag
     7741 tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt
     7801 tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca
     7861 tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg
     7921 aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt
     7981 caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg attgaccatt
     8041 cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga ccgcacacca
     8101 gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct
     8161 gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg
     8221 cgtcccgcaa gggttgcacc atcattaaca tccccggtcc gccgccgtaa ggacgatcgt
     8281 ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg atgctcagca
     8341 ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg aacatttcag
     8401 gaaacaggct aattatgcca atccacatag cgccgccttt taccgtttat ccggtggttt
     8461 aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact ttcttgataa
     8521 cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct ttcaggtttg
     8581 ccttaatgac gagaacgtca ttagatccgg tttccatcat atcaacgact ttaccgagat
     8641 cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag tagtccccct
     8701 cttcaagctg aggcagctgc gatgaatcca cgacaatttc acaattcgtc agcaggttcg
     8761 ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg tggtgcttcc
     8821 agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccag ggctgatagt
     8881 caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca cgaataccgt
     8941 aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt
     9001 tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg atcagcgcag
     9061 caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga tccagatcca
     9121 ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga
     9181 agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac ggacgctttt
     9241 tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg aataaaacaa
     9301 ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa ttttactgat
     9361 ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat ttagcgacca
     9421 ggaaatcccg gcggcatcat tcccttcatg cttctcatca tcttcgccat tccgcctttc
     9481 ttcattttct tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttaacgtcc
     9541 tgtacctgca tcccgcaacc ggcggcgata cgacgcttac gcgaaccttt gatgatttct
     9601 ggcttagcgc gctctttcat cgtcattgag ttgatgatgg cttccatacg caccagcact
     9661 ttatcgtcca tctgcgactt gacgttatcc gggatctgcc ccatgcccgg cagtttgccc
     9721 atcaagctgg ccatgccgcc catatttttc atctggcgca gttgctcaag aaagtcgttg
     9781 agatcgaagc cgtcgccttt ttttagcttg ctggctaatt tctctgcctg cgcgcggtca
     9841 actttgcttt cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac
     9901 gcgatgcggt ccggatggaa cggctccagc gcctcagttt tctcgccaac accgaggaac
     9961 ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg
    10021 tccactttgg tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta
    10081 ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc
    10141 gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg
    10201 gtatccacca gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaaca
    10261 atatcaaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc
    10321 agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cagcagaaac caccagcact
    10381 ttcttcttgt gcttctcgcg caggaaatta ccgagtttac caacgctggt tgttttaccg
    10441 gcaccttgca ggcctgccat cagcacgacc gcaggcggtt gcgcagccag attcagggtc
    10501 tggttctctt cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc
    10561 cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata
    10621 aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc
    10681 agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg
    10741 cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg
    10801 gccgcaatcg cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt
    10861 gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct
    10921 caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc
    10981 agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct
    11041 attatttctg cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg
    11101 cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg
    11161 gtgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc
    11221 attgtctatg cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac
    11281 atcacccatc tggaagcaac gcctgggatg ctgatgcaca ttggcttatc gctcttttcc
    11341 tatgccacgc taattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa
    11401 ctgaagaaca agaagctggc atttaaccag gaaatgccgc cattgatgag tatcgagcgt
    11461 aaaatgttcc acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc
    11521 ctgttctaca tgcacaacct atttagcatg gaaaatatcg acaaggctgt gctctctatc
    11581 gtggcgtggt ttgtctatat tgtgctgctg tggggacatt accatgaagg atggcgtgga
    11641 cgccgcgtcg tctggtttaa cgttgcgggc gcggtaattc tgacactggc ctacttcggc
    11701 agccgaattg tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca
    11761 tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt
    11821 ccgggtccga aaccggaatg atgaccctca accgctatcg tcttcgacat atggcgaaac
    11881 agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa
    11941 gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg
    12001 tcgggatgcg tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg
    12061 tcgtactggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag
    12121 tcgcttatcc gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct
    12181 ggttgctgaa tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg
    12241 tggttagcgg ctctttgagc aaagaagagt tgcgcactat cgtgcatgaa tcgcgctcac
    12301 aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa aaaatgaccg
    12361 ttgatgacat catggtaccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga
    12421 aatctattct gcgccaactc tcccactcac ctcacgggcg catcgtgctt taccgtgatt
    12481 cgctggacga cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga
    12541 aaaaagagtt caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgcctg
    12601 aaggtacgcc gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc
    12661 tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggttgaagat attctggaag
    12721 agattgtcgg cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc
    12781 aaaacgacgg ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct
    12841 ttaactggca tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac
    12901 tggaagagat ccctgtcgca ggcacccgcg tgcgtattgg caagtacgat atcgatattc
    12961 tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg
    13021 agagtgtggc ggagtaacga aaacggccct gcattcgcat gcagggccgt gaattattac
    13081 gaaaacagaa attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga
    13141 ccattcagcg tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca
    13201 gattccacca ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg
    13261 atcacttcaa caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg
    13321 ccttcaacca tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc
    13381 aggctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct
    13441 ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt tacgcgcaaa
    13501 ataccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt
    13561 tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg
    13621 tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta
    13681 ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga
    13741 tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct
    13801 cggaaaaatg aataatcatt tcaagtgtat tggcattgtg ggacacccac ggcaccctac
    13861 tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat
    13921 cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc
    13981 ggagattggg caactggctg atctcgcggt agtcgttggt ggcgacggta atatgctggg
    14041 cgcggcgcgc acgctcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct
    14101 gggtttcctg actgaccttg accccgataa cgcccagcaa caattagccg atgtgctgga
    14161 aggtcactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg
    14221 ccaaaaacgc atcagcaccg cgattaacga agtggtactt catcctggca aagtggcgca
    14281 tatgatagag ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg
    14341 gctaattatt tcgacgccaa caggctccac cgcctattcc ctctctgcgg gcggtcctat
    14401 tctgaccccc tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc
    14461 acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa
    14521 cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt
    14581 cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt
    14641 caatacatta agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct
    14701 ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca
    14761 ggaaaacgac tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg
    14821 agattgattt tcatagcggc atgaccgtaa taactggcga gaccggtgcg ggtaaatcta
    14881 ttgcaataga tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta
    14941 ccggcgctgc tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggccc
    15001 tgcgctggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt cgtcgcgtga
    15061 tcagcagtga tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac
    15121 tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca
    15181 ccaaacctga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctacttc
    15241 aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc
    15301 aacagttaag tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac
    15361 ttaacgaatt taatccgcag cccggagagt ttgagcaaat cgacgaagag tacaaacgtc
    15421 tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg
    15481 gtgaagacgc aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga
    15541 ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga
    15601 ttgctgaagc cagcgacgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc
    15661 gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt aaacatcacg
    15721 tcagccctga ggcattacca cagtattacc agtcgctact ggaagaacag cagcaactgg
    15781 acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat catcagcagg
    15841 cactggaaac cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac
    15901 agctgatcac cgacagtatg catgcgctct caatgccgca tgggcagttt acgattgatg
    15961 tcaaatatga cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa
    16021 ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt
    16081 cccgcatcgc actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga
    16141 tttttgatga agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc
    16201 tgcgtcaact cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg
    16261 gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacac
    16321 atatgcaatc tctggataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca
    16381 gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt
    16441 tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt
    16501 aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc
    16561 ccgaaaagga atcaaatcac tatgcgctgt aaaacgctga ctgctgcagc agcagtacta
    16621 ttgatgttaa ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac
    16681 caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa
    16741 caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg
    16801 ttctatgtct tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgctg
    16861 acctttaaca gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac
    16921 taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg
    16981 tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa
    17041 tgagagggcg ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg
    17101 gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc aattccagca
    17161 agccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc actcgctgca
    17221 ggtattgctt ctcaggtagc gcataagcca cctcaacggc aattttacct agcactgtaa
    17281 acctctttcg cacgaaccgt aaaagcctgg accatattag ccgccagctc tttaaacacg
    17341 cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata
    17401 cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc
    17461 ccatccacca gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac
    17521 gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac
    17581 tccagaatcc gacttccggt acaacctggc aaaaactgag gataagactg aacgtcattc
    17641 actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc
    17701 ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa
    17761 acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca
    17821 ggattgatat ggggtgtttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa
    17881 gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga
    17941 atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg aagttaaatc
    18001 cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc
    18061 atttctgttt ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga
    18121 tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg
    18181 cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg
    18241 caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa
    18301 agaacgcgaa tggcaggtgg ataaagcacg catcatgaaa aacgcccacc gttaaacctg
    18361 cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat
    18421 cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat
    18481 caggctacat gggtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca
    18541 taaatctggt atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg
    18601 aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc
    18661 aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc
    18721 ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc
    18781 tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg
    18841 cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc
    18901 ggacgcgggt tcaactcccg ccagctccac caaaattctc catcggtgat taccagagtc
    18961 atccgatgaa gtcctaagag cccgcacggc gcaagccctg cgggcttttt tgtgctccga
    19021 atttgtcccg cgaagtctga tgccaactaa ttaaatccga tccttttagg caccttgtta
    19081 ggcacttcat aaagctatat tgtctttgag gtgcctaaaa ctatggaaac ccggcaatgg
    19141 caagacaaac caaacctcta tccgttaagg aaatcgaatc tgctaaaccc aaggaagcgg
    19201 actacgttct ctatgatggc gatggccttg agctactcat taaatccagc gggagtaaaa
    19261 tctggcagtt tcgctacatt cgccctgtca ccaagacacg agcaaagaaa agcataggcc
    19321 cttacccgtc agttaccctt gccgatgcca gaaactaccg tgcagagtcc cgctcactcc
    19381 tagctaaaca gattgagccg caggaacatc agcaagaaca attgcgtagt tcgctggaag
    19441 ccaaaaccaa tactttccag ctcgtagctg aacgttggtg gaatgtgaag aaagccagtg
    19501 tgacagagga ctatgcggaa gatatctggc gctctcttga aagagatgtc tttcctgcga
    19561 ttggcgacgt tagcgttaca gatattaaag ctcatacact ggttcaggcc gtacaaccgg
    19621 ttcaggccag aggagcactg gaaacggttc gtcgcctgtg tcaacgtatt aatgaggtca
    19681 tgatctatgc ccagaacaca gggctgattg atgctgttcc cagcgttaat atcggtaaag
    19741 ccttcgaaaa gcctcagaaa aagaacatgc ccagcattcg accggatcag ctatctcaac
    19801 tgatgcagac aatgcgaaca gccagcatta gcctttccac acgctgcctg ttcatgtggc
    19861 aacttcttac tattacccgc cctgccgaag cggctgaagc tcgctgggaa gaagtagaca
    19921 tagaagcgca agagtggaag attcccgcag cacgcatgaa aatgaaccgc gaccatactg
    19981 ttccattgtc agatgaagca atagctgtgc tggagatgat gaaaccgcta agcggcaacc
    20041 gagaatttat ctttcctagc cgtatcaagc ccaaccagcc tatgaacagt cagactgtta
    20101 acgcctcact aaaacgcgca ggttttggtg gtgtgcttgt ttcgcatggt ctgagatcaa
    20161 ttgccagtac ggctcttaat gaacaaggct ttccgcctga tgccattgag gcagcattgg
    20221 cccatgtgga taagaatgag gttcgtcgtg cttataaccg cagcgattac ctggaacagc
    20281 gtcgcccgat gatgcaatgg tgggcaaatt ttgtgatggc agcggatcgt ggaagcatga
    20341 ttgaaggtgg gataaaagga atgaagctag ttgggtgact aaatagctgc gcggcatagt
    20401 gtcccacttt cagccagccc ttgcaggtta agccaccaga cagtaaacgg cgggtgtcgc
    20461 gttattaggc gcagctattt agtagtaata ttaggattgg tgattctgca ctccagaagt
    20521 t
//
LOCUS       DAEEUE010000072        20488 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.072, whole genome shotgun sequence.
ACCESSION   DAEEUE010000072 DAEEUE010000000
VERSION     DAEEUE010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20488
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.072"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            112..1167
                     /gene="phoE"
                     /locus_tag="KLK84_002770"
     CDS             112..1167
                     /gene="phoE"
                     /locus_tag="KLK84_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HBE2010033.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            complement(1206..1607)
                     /gene="crl"
                     /locus_tag="KLK84_002771"
     CDS             complement(1206..1607)
                     /gene="crl"
                     /locus_tag="KLK84_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HBE2010034.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(1665..2909)
                     /gene="frsA"
                     /locus_tag="KLK84_002772"
     CDS             complement(1665..2909)
                     /gene="frsA"
                     /locus_tag="KLK84_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HBE2010035.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPKLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(3001..3459)
                     /gene="gpt"
                     /locus_tag="KLK84_002773"
     CDS             complement(3001..3459)
                     /gene="gpt"
                     /locus_tag="KLK84_002773"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBE2010036.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3721..5178
                     /gene="pepD"
                     /locus_tag="KLK84_002774"
     CDS             3721..5178
                     /gene="pepD"
                     /locus_tag="KLK84_002774"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HBE2010037.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5235..5849)
                     /locus_tag="KLK84_002775"
                     /pseudo
     CDS             complement(5235..5849)
                     /locus_tag="KLK84_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(5911..6954)
                     /locus_tag="KLK84_002776"
     CDS             complement(5911..6954)
                     /locus_tag="KLK84_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBE2010038.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCLGRPD"
     gene            complement(7200..7652)
                     /locus_tag="KLK84_002777"
     CDS             complement(7200..7652)
                     /locus_tag="KLK84_002777"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE2010039.1"
                     /translation="MNNIQIRDYHPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            complement(7662..8060)
                     /gene="yafO"
                     /locus_tag="KLK84_002778"
     CDS             complement(7662..8060)
                     /gene="yafO"
                     /locus_tag="KLK84_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HBE2010040.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(8063..8356)
                     /gene="yafN"
                     /locus_tag="KLK84_002779"
     CDS             complement(8063..8356)
                     /gene="yafN"
                     /locus_tag="KLK84_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HBE2010041.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            complement(8408..9463)
                     /gene="dinB"
                     /locus_tag="KLK84_002780"
     CDS             complement(8408..9463)
                     /gene="dinB"
                     /locus_tag="KLK84_002780"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBE2010042.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(9534..10319)
                     /gene="lafU"
                     /locus_tag="KLK84_002781"
     CDS             complement(9534..10319)
                     /gene="lafU"
                     /locus_tag="KLK84_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HBE2010043.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            10291..12003
                     /locus_tag="KLK84_002782"
                     /pseudo
     CDS             10291..12003
                     /locus_tag="KLK84_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(12220..12717)
                     /gene="rayT"
                     /locus_tag="KLK84_002783"
     CDS             complement(12220..12717)
                     /gene="rayT"
                     /locus_tag="KLK84_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HBE2010044.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(12893..13414)
                     /locus_tag="KLK84_002784"
                     /pseudo
     CDS             complement(12893..13414)
                     /locus_tag="KLK84_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            13798..14538
                     /locus_tag="KLK84_002785"
     CDS             13798..14538
                     /locus_tag="KLK84_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HBE2010045.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(14509..15276)
                     /locus_tag="KLK84_002786"
     CDS             complement(14509..15276)
                     /locus_tag="KLK84_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HBE2010046.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWHLFCLGERVV"
     gene            complement(15482..16060)
                     /gene="lpcA"
                     /locus_tag="KLK84_002787"
     CDS             complement(15482..16060)
                     /gene="lpcA"
                     /locus_tag="KLK84_002787"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HBE2010047.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            16300..18744
                     /gene="fadE"
                     /locus_tag="KLK84_002788"
                     /pseudo
     CDS             16300..18744
                     /gene="fadE"
                     /locus_tag="KLK84_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
     gene            complement(18787..19233)
                     /gene="ivy"
                     /locus_tag="KLK84_002789"
     CDS             complement(18787..19233)
                     /gene="ivy"
                     /locus_tag="KLK84_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HBE2010048.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            19414..20184
                     /gene="yafV"
                     /locus_tag="KLK84_002790"
     CDS             19414..20184
                     /gene="yafV"
                     /locus_tag="KLK84_002790"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HBE2010049.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            20294..20488
                     /locus_tag="KLK84_002791"
                     /pseudo
     CDS             20294..20488
                     /locus_tag="KLK84_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541010.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
BASE COUNT         5010 a         5265 c         5269 g         4944 t
ORIGIN      
        1 aatatcatta atctgtaata tatctttaac aatctcaggt taaaaacttt cctgttttca
       61 acggggctct cccgctgaat attcgcgcgt taattaaaat caggaatgaa aatgaaaaag
      121 agcactctgg cattagtggt gatgggcatt gtggcatctg catctgtaca ggctgcagaa
      181 atatataata aagacggtaa taaactggat gtctatggca aagttaaagc catgcattat
      241 atgagtgata acgccagtaa agatggcgac cagagttata tccgttttgg ttttaaaggt
      301 gaaactcaaa ttaacgatca actgactggt tatggtcggt gggaagcgga gtttgccgga
      361 aataaagcgg agagtgatac tgcacagcaa aaaacgcgtc tcgcttttgc cgggttgaaa
      421 tataaagatt tgggttcttt cgattatggt cgtaacctgg gcgcgttgta tgacgtggaa
      481 gcctggaccg atatgttccc ggaatttggt ggcgattcct cggcgcagac cgacaacttt
      541 atgaccaaac gcgccagcgg tctggcgacg tatcggaaca ccgacttctt cggcgttatc
      601 gatggcctga acttaaccct gcaatatcaa gggaaaaacg aaaaccgcga cgttaaaaag
      661 caaaacggcg atggcttcgg cacgtcattg acatatgact ttggcggcag cgatttcgcc
      721 attagtgggg cctataccaa ctcagatcgc accaacgagc agaacctgca aagccgtggc
      781 acaggcaagc gtgcagaagc atgggcaaca ggtctgaaat acgatgccaa taatatttat
      841 ctggcaactt tctattctga aacacgcaaa atgacgccaa taactggcgg ctttgccaat
      901 aagacacaga actttgaagc ggtcgctcaa taccagtttg actttggtct gcgtccatcg
      961 ctgggttatg tcttatcgaa agggaaagat attgagggca tcggtgatga agatctggtc
     1021 aattatatcg acgtcggtgc tacgtattat ttcaacaaaa atatgtcagc gtttgttgat
     1081 tataaaatca accaactgga tagcgataat aagctgaata ttaataatga tgatactgtc
     1141 gcggttggga tgacgtatca gttttaatga atattgccgg atgtgatgca tccggcacat
     1201 ttcactcacg ccgttaactt caccggttca tcacgaaaat cattcgccgg ttccagcttc
     1261 agattcagcg tcgtcagcag ttcacgcagc ttctcgtgaa actcacgcag ggtgtgctcc
     1321 agtcgttcaa ccacttcagt atcttttacc ggaacactct tccagtcgcc tgctttatcg
     1381 aacagaccaa actggtagct gtaggtaaaa cgggattcct gcgcttccag ctccatccac
     1441 cagccccaga attcacgcac ttccggtgcc ggtttcacgt tgacgcatac agccagacaa
     1501 tcgaaaaaga atcgattatc ttcgcactta ccttcacgaa tatacgggcc tagtgcggta
     1561 aattttttga tcaatctgct cttcgggtgt ccactcggta acgtcattgc tatctcctgt
     1621 tgtgatgcaa ctgttttacc aaattggcaa aatttagcaa atttttaaca caagcgtttt
     1681 tcgatccagc cggtgatttc ctgaagacct ttgtcaaaat tccgatacac cgggttaaat
     1741 gggatctcta ataatttacc gtcagcagat gatgaggtga ttaagcgtga gtcctcttcc
     1801 gggctgaacg gatcgttctt ccagtagcct gataacattg gcgttgggca gcgacgcccc
     1861 agcaatcctt gcacttttaa tgaatagcga ttcagctcca cgcgtaacgc ttcgtcggaa
     1921 gcatcatgca tccccaaacg actcgccaga acgtcaagat acatttccgg cacctgttgc
     1981 tggcacttaa aatcactcaa cagggtatga actaccggac caagacaggc aaccgctttc
     2041 agacgcggcg attcaaggta tgccagacgc acggcaacgt tagcgccgaa acggaaacca
     2101 aaggccgcga cgcgagtgtg atccacccac ggtacgttag gcagcgcctt taagacgtgc
     2161 tgatgcaaca ggctggagtc ctgggtgagc ttccattttg aagaaaagcc caccgacggc
     2221 atatcaatag tcagcatcgc aatgccgcgc ggcgcaaaat aacgttcata caggctgtaa
     2281 tagtccgtct gcatcgcatc cagaccacca cacattaata ctgtcgggaa cgggccatcg
     2341 cctttcggca tatgcaaaaa gccggtgatg ggcgcaccgc cgggtacggt aaactccatc
     2401 tgccgcatcg tgcccggcag acgctgggcg gcctcttcat aggcgcggtt tgataaagcc
     2461 tgcgcttgct cggccaggtc atctcctttc agatgaggat aggcggcaat gttgtacaac
     2521 gtagccgcat gcagccagtg tcgaccactg agtttcggat cttcttccgt acaggctttt
     2581 tgttgccaca ccatcgcctg ggtggcccac tcataaatcc agttgccacc acggtagcca
     2641 atcacggtat cgtataaatc atcgtcggta cgttcagcat cgctcatcac aatacgtgcc
     2701 tggacgtcga ggatttcacg tgggtcaata ccgcgccaga tccacatcag acggttaatc
     2761 atgcgatacc agtgagggat ggttttacca tcaagagccg actgcacagg cggttgtgcg
     2821 ccgtgattaa agcggcggac tagcgtcgag gtttcaggat gtttaaagcg gggtttgaac
     2881 agggtttcgc tcaggtttgc ctgtgtcatg gatgcagcct ccagaatact tactggaaac
     2941 tattgtaacc tgcctgaagt taaaaagaac aacgcccggc aatgccaggc gttgaaaaga
     3001 ttagcgaccg gagattggcg ggacgaatac gacgcccata tcccacggct gttcgatcca
     3061 ggtatcttgc gggatatcaa caacatagtc atcaaccagc ggacgaccag ccggttttgc
     3121 gaagatggtg acaaagtgcg cttttggata catttcacga atcgcaacag cagtaccacc
     3181 ggtatccacc aggtcatcaa taacgatgaa gccttcgcca tcgccttctg cgcgtttcag
     3241 cactttaagc tcgcgctggt tgtcgtgatc gtagctggaa atacaaacgg tatcgacatg
     3301 acgaataccc agttcacgcg ccagtaacgc ccccggtacc agaccgccac ggcttacggc
     3361 aataatgcct ttccattgtt cagaaggcat cagtcggctt gcgagtttac gtgcatggat
     3421 ctgcaacatg tcccaggtga cgatgtattt ttcgctcatg tgaagtgtcc cagcctgttt
     3481 atctacggct taaaaagtgt tcgaggggaa aataggttgc gcgagattat agagatctgg
     3541 cgcactaaaa accagtattt cacatgagtc cgcgtctttt tacgcactgc ctctccctga
     3601 cgcgggataa agtggtattc tcaatacata tctcgcaagc ctgtcttgtg ttgacaacat
     3661 tttctgctaa ccctgtgacc tgcaatactg ttttgcgggt gatcgacaag gagacttaac
     3721 gtgtctgaac tgtctcaatt atctccacag ccgctgtggg atatttttgc caaaatctgt
     3781 tctattcctc acccgtccta tcatgaagag caactcgctg aatacattgt tggttgggca
     3841 aaagagaaag gtttccatgt cgaacgcgat caggtaggta atatcctgat tcgtaaacct
     3901 gctaccgcag gtatggaaaa tcgtaaaccg gtcgtcttac aggcccacct cgatatggtg
     3961 ccgcagaaaa ataacgacac cgtgcatgac ttcacgaaag atcctatcca gccttatatt
     4021 gatggcgaat gggttaaagc gcgcggcacc acgctgggtg cggataacgg cattggtatg
     4081 gcctctgcac tggcggttct ggctgacgaa aacgtggttc acggcccgct ggaagtgctg
     4141 ctgaccatga ccgaagaagc cggtatggac ggtgcgttcg gcttacagag caactggttg
     4201 caggctgata ttctgattaa caccgactcc gaagaagaag gtgaaatcta catgggttgt
     4261 gcggggggta tcgacttcac ctccaacctg catttagatc gtgaagcggt tccagctggt
     4321 tttgaaacct tcaagttaac cttaaaaggt ctgaaaggcg gtcactccgg cggtgaaatc
     4381 cacgttggcc tgggtaatgc caacaaactg ctggtgcgct tcctggcggg tcatgcagaa
     4441 gaactggacc tgcgccttat cgatttcaac ggcggcacac tgcgtaacgc catcccgcgt
     4501 gaagcctttg cgaccattgc tgtcgcagct gataaagtcg acgcactgaa atctctggtg
     4561 aatacctatc aggagatcct gaaaaacgag ctggcagaga aagagaaaaa tctggccttg
     4621 ttgctggact ctgtagcgaa cgataaagct gccctgattg cgaaatctcg cgataccttt
     4681 attcgtctgc tgaacgccac cccgaacggt gtgatccgca attccgacgt ggcaaaaggt
     4741 gtggtcgaaa cctccctgaa cgtcggtgtg gtgaccatga ctgacaataa cgtagaaatt
     4801 cactgcctga tccgttcact gatcgacagc ggtaaagact acgtggtgag catgctggat
     4861 tcgctgggta aactggctgg cgcgaaaacc gaagcgaaag gcgcatatcc tggctggcag
     4921 ccggacgcta attctccggt gatgcatctg gtacgtgaaa cctatcagcg cctgttcaac
     4981 aagacgccga acatccagat tatccacgcg ggcctggaat gtggtctgtt caaaaaaccg
     5041 tatccggaaa tggacatggt ttctatcggg ccaactatca ccggtccaca ctctccggat
     5101 gagcaagttc acatcgaaag cgtaggtcat tactggacac tgctgactga actgctgaaa
     5161 gaaattccgg cgaagtaatt atttgatttg ctgccggatg gcgtttaatc gccttccggc
     5221 agtttcatcc ttcattatcc ttcgataaaa gccatccctg taaacgttcg tcgcgggttg
     5281 ccacgttcaa tctggtgatg gaacattcgc cgctgtgatt tcagcaccgc gctattttcc
     5341 tattgctgct gatccagctt ccaggcaatc agcaatcgcg ccagccgttt gttagcatgc
     5401 tggctgcgct ctgactgaac cttcacgcta ataccggatg ccaaatgcgt ggcgcgtacc
     5461 gccgagtcgg ttttattgac atgttgaccg cccggccccg acgaacgcag cgtctcatac
     5521 cggatcgcat cggattgctc ctgctcatca gcggtaaaac gcccaatgcc cagaaaccag
     5581 tttttacgcc cgtgatgtgg gcgatacggg ctcgggcaaa tccactgaat agtgccacac
     5641 cagctttcgc ttaacgccca tgcgttgtcg ccatccagag aaatcagcgc cgaacgcagc
     5701 gtgtcagagt agcggcctgt ttctgtttcc agcaccgtta ccgcgacgtc ttgtcgggca
     5761 gcttctttaa ttagcctgtc cagcgccttt ttcactgcga gacaacattc ttccggcccc
     5821 tgcgctgagg agagttatag caagatcatc cttttttccc tccactgttt ttgagcgtta
     5881 gcaccggacg cagccgcgcg acaggaataa ttaatccggc ctgcccagac attgcaccac
     5941 actttcagcc gatttatagg cttgtggcgc ttcttcgaag atgagttgtt tatcgcgaca
     6001 aattacccga ctgccaagtt cagtacgcga gagttgcgtc gctgtgtatt tcgctgccag
     6061 acgcccttta cactcggtgc gcccccattt acgcccagcc ccatgcgcca gcgaatgcaa
     6121 tgtttcctca ctcctgacgg gctgaaccag cctggagtaa tcaccgcgtg aaccgggaat
     6181 aaccaccaga ccacagtcat caggcgtggc acctttacga tgtaagaccc cttgttgatc
     6241 accgattcga cacgcgctaa caaagttatg cgccacatcc agaaccggac ttccggtggc
     6301 cttaacctgt tgcattatgc gcaaagcgat caggtggcga ttaaaacgcg caaacgccag
     6361 cgcgtcgtca ctgccttcag gcaaaccatg atgcgaaaac gaggcaatat gccgctgtaa
     6421 aatagactgc cctagccccc tcgagccgct atgaaccagc agttgcagat gctgcgcatc
     6481 cagacctgcc agtgcaaaca gttcagcgtt gatgatttga tcaacttgtt gcagttcagc
     6541 gaaatgattg ccaccaccga tagagccaag cgagctgcgc cacggatgct gtgcaaatgc
     6601 tgacggcagg ttttcctcca gccagctttc ttcagcaacg tcatccaaag ctgataatcg
     6661 cttttcaaac ttatcggcgt tgtatttgcg agcgagaata tctgtttgcc atagcgccat
     6721 gccacagccg atatcattgc cgaccagtgc tgggtaaaaa cggcctgcgg agaagaacgc
     6781 tgcgccaatc gggtagccgc gtccggggtg taaatctggc atccccacta cgcgctgcat
     6841 gttgggtaaa tttgccgtgg tgtgtaattg ttggatcgct aaactctcga tccacaggtc
     6901 atcagatgcg atggtaaata ccgcatcgga taagggacga atataattgc ccatatacca
     6961 ttccgtaaat agactcgtca tacttcaagt tgcatgtgct gcgacttgaa ttattgagag
     7021 taacaattaa aaaatcagga aatggcagac gaatccaggg caagaaaaaa agaattaatc
     7081 ctggaaaggt ctgcaaagag tcattgataa aggatttgtc atgatttacc tccttgcagt
     7141 ttgtggaata agaaaacgcg cacatactag gcgggcgtta ttttcattgc aagctggatt
     7201 taatgttgcg gtttataacg catataaaaa ttaataaacc acgctccccg gcattcaacg
     7261 cgctgctgct taactgtctg aaaaccataa cgttcaaaaa agggttttgc ggttatgctt
     7321 gcgtccaccg taagttcgga ttcagactta ataaaaggtt ttaacaaagc gctggcaacc
     7381 ccacggcggg tgtattcagg gtcaacaaat aacatatcga tatagtgttc aacgcaggta
     7441 ataaagccga ccagttttgc attaataact gcaacccgca cttgtgattt cgcgagtttc
     7501 tccttccagc gagattcgtc aatctgcgcc caggcggcaa tttgttgtag tgaataatgc
     7561 tgactgacgg tcatcgtaac cgctctaatg aaaatagcgc atagttgctg aaaatcgcca
     7621 ggatgataat ctcttatttg tatgttattc ataatataaa ttcaaaaacg catgcgaaac
     7681 gcttctgcca tttttccaat tttatgcatt tggttgttat ctcgagccag tttatgaggt
     7741 tcaggcttca gaatggcaat gagcaaccat gcttgctcat caaacgcacc ctgacaatat
     7801 accaaatgcg cttcgtcatt cgttctgctg aattggcgca actgtggcgg aaatggatta
     7861 ttcacatttg ccagatgaat atgagcaact cgctcaaatt tgattaatgg ccaggtaaac
     7921 gagtcgtcgt agagtgcatc gcgaccaaat atatctggca aaacaccgtc acgcttatag
     7981 gaaataaaat ccgccgttaa cgcatcaagt tcctctgctg taagttgcag gcgaataagt
     8041 tttgttttga atacccgcat ccttattcct taaagtcatt gaaatcatca tccgtcatat
     8101 cattaagata ttgtttagcg cggcgtgtaa tttcctctaa tcttgcagca cttggacgaa
     8161 agcgcgcctt tatgggcttt ttctcctctt gttcagcaag catgtccatt aacgcttcga
     8221 atgcgctggc acttaagaga tatcctgcgg ggcgattatt agaaagaacc gcaactggtt
     8281 gatcaataaa gtatttagct gggtttttac gtaactcagt gatattgacc gatttttcag
     8341 cgagaattcg atgcatacag tgataccctc ataataatgc acaccagaat atacataata
     8401 gtatacatca taatcccagc accagttgtc tttccatttg cgggtcaagc aacgtcacat
     8461 gcagccccac cagacgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     8521 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgggtg gtctgctgaa
     8581 aatcgtcgaa ctttaatttc accccctggc gagcaatcag taaatcaggt tttacctttg
     8641 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     8701 gatgaatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgtaac cgttcgctgt
     8761 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     8821 agcgtttaag cagaatcacc agatcacact tttgtacatc accgcaggtc cgcagcccca
     8881 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gattttttcc agtggtaagg
     8941 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctgaccgttg ggtttattca
     9001 tgtcggaggc gattttggcg agaaacttta ctggtgccac gcccgcagat gccgtcagtt
     9061 gcagctcgtt gaagatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     9121 aatggacgct atcggtgaca tcgagataag cctcatccag tgacaacggt tcaatgcgcg
     9181 aggtgtagcg cgagaagatt tcacggatat gatttgaggc ttctttgtag gcgtcaaagc
     9241 gccccggaag caaggtgaga tgtgggcata atttgagcgc catcccagta ggcatagcgc
     9301 tacgtacgcc aaatttacgc gcgggataat tggcggtgct gatcaccccc cgacgttcgc
     9361 ggctgccgcc aatagcaata gggatatcgc gcagagcggg attgtcgcgc atctccaccg
     9421 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     9481 tggtaaagta tacagtgatt tcaggttttg agaaatgcgt aaagattcag catttagcgc
     9541 attcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     9601 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     9661 atctcaatac gccgattgcc cgcgctttgc ggattcttcg cgtccagcag catctggtcc
     9721 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     9781 cgagctgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtag
     9841 gccatcgcat cggtatgccc ggtaataata attttgttat agagcgagtc gaacactggc
     9901 gcgagctcca ccagcaaagt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     9961 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
    10021 tccagattcg cttccatatg cgcatcacgg gcaatggtgt tgatactggt tgccaactcg
    10081 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattcac atcacgcgct
    10141 tttttctccg tctcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
    10201 attttattta acggcgacag cccaccaccg ttaaaaatcg actgcccgtg cagcgcggcg
    10261 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat cgtcgtcacc
    10321 aaagaggccg agcgtatttc cgaggtttct gcccgcttta ccctggacgc gatgcccggc
    10381 aaacagatgg cgattgacgc cgatcttaac gccggattga tcaaccaggc gcaggcgcaa
    10441 acccggcgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
    10501 aagtttgtac gcggagacgc catcgccggg atgatgattc tggcgatcaa cctgagcggc
    10561 ggcgtctgta tcggcatctt caaatacaac ctgagcgccg acgccgcctt ccagcaatac
    10621 gtgctgatga ccatcggcga cggcctggtg gcgcagatcc cttccctgct gctctccacc
    10681 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgc
    10741 caccagttgc tggcaagccc gtcggtgctc tacaccgcca ccgggatcat gttcgtgctg
    10801 gcggtggtgc cggggatgcc gcatctgccg tttttgctgt tcagcgccct gcttggcttt
    10861 accggctggc ggatgagcaa acgcccgcag gcggcggagg cggaagagaa aagcctcgaa
    10921 acgctgaccc gcaccatcac tgaaaccagc gaacaacagg tcagttggga aactattccg
    10981 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcg
    11041 cagggcaacc cgctcaccca gcggattcgc ggcgttcggc aggtgatttc cgacggtaac
    11101 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gcctcaagcc cagccagtac
    11161 gctattttca tcaacggcat tgaggctgat gaagcggata ttccggcgga taaactgatg
    11221 gcgctgccct ccagcgaaac ctacggcgag attgacggcg tgctggggaa cgacccggcg
    11281 tacgggatac cggtcacctg gatccagcct gcgcagaaag cgaaggctct gaatatgggg
    11341 tatcaggtga tcgacagcgc cagtgtgatt gccacccatg tgaacaagat tgtgcgcagc
    11401 tatattcctg atttgtttaa ctatgatgac atcacgcagt tgcataaccg tttgtcgtcg
    11461 atggcaccgc gcctggcgga ggatttaagc gcggcgctca attacagcca attgctgaaa
    11521 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
    11581 gtgctggtcg ccagtagcac ggtgactaaa gatcatattc tgctggcggc cgatgtgcgc
    11641 ctggcgctgc ggcgcagcat tactcatccg ttcgttcgca agcaggagct gacggtgtat
    11701 acgctgaata atgagctgga aaatctgctg accaacgtag tgaatcaggc gcaacaggcc
    11761 ggcaaagtga tgctcgacag cgtgccggtt gacccgaata tgctcaacca gttccagagc
    11821 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagatccggt gctgctggtg
    11881 ccgccgcagc tacgcccttt gctggcgcgt tatgcaaggt tgtttgcgcc ggggctgcat
    11941 gtgctgtcgt ataacgaagt gccggatgag ctggagttga agattatggg ggcgttgatg
    12001 taagaatccg gcatcggtgc acaattgccg gatgcggcgt aaacgcctta tccggcctac
    12061 cgttcaggca caagttttta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg
    12121 cataactgcc ggatgcggcg tgaacgcctt atccggccta ccgttaaagc acgatttgta
    12181 ggccggataa gcgtaagcgc atccggcacc agcgcacagt tataaaatga tacgctcccc
    12241 ggcacttaaa tccgttatgt ctcccgccca atcgatggga tataaccctc gcgcgacatc
    12301 gcgatggaac gttgagaatg gccaatcact cacctgcttt acccagccat gtttctctgg
    12361 atttatataa atataatcaa catgatggcg ataatctttt gtattgcgaa tggcatgctc
    12421 ccaaaaacgc ggttgccaga tatttttcaa tccgcaagca tgggtgaact gctttttaat
    12481 ctcccgccag cgagaggaaa aatcatcatc gctttcgggt aatgtccata tgcaatgcat
    12541 atgctccggc aaaacgaccc aggcgttaat ttcaaaaggt ctgtctcgct taacgttaat
    12601 aatggcgtta cggagcgtct ggaactgggt ggtcagaaga tggcttcgac gattgcgtaa
    12661 atttaccgtg aaaaaccatg ttcccccctt gatgtaataa cgacgatatt ccgacatagc
    12721 gactcctttt tctcaacggt aagaggaaaa ggaaaggccg acaatgacgc tgtgttatat
    12781 cgggaaagcg gctcgcaaaa acgcacagat ttgtaggcat gataagacgc gtcagcgtcg
    12841 catcaggcat ccgtgcacaa ccgccggatg cggcgtaaac gccttatccg gcctacaaaa
    12901 tcatctcttc cgtcaaaatc ctgcgcgcgc ctaaaaaatg gtcctgccag aaaggttcgg
    12961 ctaaccggct tacccgaatg gtttccccgg tgcgcagtga ctcaataaac tgaccgtcgc
    13021 ccagatacac ccccatatgg tcggcaatct cgcggctgtg gatatggaaa aatagcaaat
    13081 ctccccggcg caggtcgtta ttcgccacaa tcgttgcccg acggtagtgg tacatctcat
    13141 tggccgtgcg cgggagtttc gcctcaagga tcttgttgta ggcataaaaa actaacccac
    13201 tacaatcgaa gccttgatcg ggccgcgtgc cgccccagac gtaaggcttg ccaagctgct
    13261 gctccaggcg gtgaatcgcc acttcggtga tgttatgcag gtgatcgctg cttaaaaagt
    13321 ggttgttttc cgccagcgcc tgccagcgtc tgtcactcgc cttgagcggc gcaggaaacc
    13381 attctggatg ctgttttatc tgctcccggt tgtggtggcg ctggcgagta aagggagcaa
    13441 aacgcataac gaaaacacgc ctggatgcaa aaatctgccg agcaagaacg aagacatgaa
    13501 agacataaac cggctgaatg aaggtgagaa ttttaaaaga ccggaggagt aaacgggatt
    13561 attaattgct tatttgcata ttactattga gatttcataa tattaggaaa taaatgatac
    13621 atgcgctttt cggactattg aaaactgccc ctgtgctaaa tttgtaaacc cagcgccgac
    13681 aggcttgcgt aaaactcttt tggtgataat gtgagccttc caaatccaga ctattccgat
    13741 ttgggatcca ttttgccgtc ctggcgtgtg taaccgtttt atcaaggaat aagcagtatg
    13801 cgtaaaatcg cattaattct tgcgatgttg ttgatcccgt gcgtttcgtt tgccggtttg
    13861 ctgggcagca gtagctcgac cacgccagtg agcaaagagt ataagcagca gttgatggga
    13921 tcccctgtct acatccagat cttcaaggaa gaacgtacgc tcgatcttta cgtcaaaatg
    13981 ggcgagcaat atcaactgct cgacagttat aaaatctgta aatattccgg cggcttaggg
    14041 ccaaaacagc gtcagggcga tttcaaaagc ccggaagggt tttatagcgt ccagcgtaat
    14101 cagttaaaac cagacagccg ttactacaaa gcgattaata ttggattccc caatgcttat
    14161 gaccgtgcgc atggttatga agggaaatac ctgatgatcc acggcgattg tgtatccatc
    14221 ggctgctacg caatgaccaa tcagggtatt gatgagatct tccagttcgt tactggtgcg
    14281 ctggtgtttg gtcagccgag cgtgcaggtg agtatttacc cattccgcat gaccgacgcc
    14341 aatatgaagc gccataaata ttccaacttt aaggacttct gggagcagct gaagccgggc
    14401 tacgactact ttgagcaaac ccgtaagcca ccgaccgttt ctgtggccaa tggccgttac
    14461 gtggtcagca agccgttaag ccacgaagtg gtgcagccac aactggcatc aaactacacg
    14521 ctccccgagg caaaataagt gccattcgcc tggcataatt ttttgccagg tttcattgcc
    14581 cgtcagcggc tgtgtcgcaa tcaccgtgac cacatcattc ggtgtggtct gcgagctgaa
    14641 gtcgatttcc acatcctgat ccagcaacgt tgccacgcca aacggcgcgc ggcgggtgat
    14701 ccagtgtaaa ttagtcgagc aatacgccat tacatagcgc ccgtccgaaa gcagcatgtt
    14761 gaaaacgccc ttcgcccgca gttcatccgc cagtgaggcg atatatttaa acactgccgc
    14821 catgttgccc ggcgtgcgcg ggtaacgctg cgttaattta tgcagcagcc agcaaaaggc
    14881 tttttcgctg tcggtttcac cgaccgggcg gaagttgccg gtttccagtg atttgtagcc
    14941 cgtcagttgt ccgttatggg cataagtcca gttacgcccc cataactcgc gggtaaatgg
    15001 gtgagtattt tccagcgcca cctcgccccg attagcctgg cgaatatgag ccaccaccga
    15061 acaggatttt atcgggtagt cctggacaag tttggcgatg ggggaattaa agctgggttg
    15121 tggatcttta aatgtgcgac agcctttacc ttcgtaaaag gtaatgcccc agccatcttt
    15181 atgtggcccg gttccaccac cacgctgtac aagtccggtg aaactaaagc agatatcggt
    15241 agggacgttg gcgctcatcc cgagcaattc gcacatgggt aacctccaca acagcgggag
    15301 cgcatggacc ccggcgaaga ttgatttaag aattttcagg tcggatgcgg cgtaaacgaa
    15361 ttattcggcc tacggttcgg gtacagattt gtaggcatga taagacgcgt cagcatcgca
    15421 tcaggcatca gcgcacaagt gccggatgcg gcgtaaacgc cttatccggc ctacgccaga
    15481 cttacttaac catctctttt tcaattaact ggatcaggat atggatcact ttaatgtgaa
    15541 tctcctgaat gcggtcggca taaccaaagt gcggaacgcg aatttcgata tccgccgtgc
    15601 cagccatttt accgccgtct ttaccggtca gggtgatcac tttcatcccc ttctcacgcg
    15661 ccgctgcgat cgctttgatc acgtttgcag agttaccgga ggtggagatc cccagcagta
    15721 catcgccttc gcgacctacc gcttcaacgt agcgggagaa aatatcattg aaaccgaaat
    15781 cattaccgac gcaggaaata tgactaacgt cagaaatagc aatcgccggg tagcccggac
    15841 ggttttcacg gtagcgaccg gtcaactctt cggcaaagtg catagcgtcg caatgggaac
    15901 cgccgttgcc gcaggaaagc actttgccac cggctttaaa gctgtctgct aacaggaccg
    15961 ccgcgcgctg aatggcgtga atattggcgt catcttttaa aaagttagcc agcgtttccg
    16021 ccgcttcgtt cagttcgtta cgaataagat cctggtacat gaggatatcc ttcagcataa
    16081 atgtaataga caaaatgcag tgtaccggat accgccaaaa gcgagaagta cgggcaggtg
    16141 ctatgaccag gactttttga cctgaagtgc ggataaaaac agcaacaatg tgagctttgt
    16201 tgtaattata ttgtaaacac attgctaaat gtttttacat ccactacaac catatcatca
    16261 caagtggtca gacctcctac aagtaagggg cttttcgtta tgatgatttt gagtattctc
    16321 gctacggttg tcctgctcgg cgcgttgttc tatcaccgcg tgagcttatt tatcagcagt
    16381 ctgattttgc tcgcctagac agccgccctc ggcgttgctg gtctgtggtc ggcgtgggta
    16441 ctggtgcctc tgaccattat cctcgtgcca tttaactttg cgcctatgcg taagtcgatg
    16501 atttccgcgc cggtatttcg cggtttccgt aaggtgatgc cgccgatgtc gcgcactgag
    16561 aaagaagcga ttgatgcggg caccacctgg tgggagggcg acttgttcca gggcaagccg
    16621 gactggaaaa agctgcataa ctatccgcag ccgcgcctga ccgctgaaga gcaagcgttt
    16681 ctcgacggcc cggtagaaga agcctgccgg atggcgaatg atttccagat tactcatgag
    16741 ctggcggatc tgccgccgga gttgtgggcg taccttaaag agcatcgctt cttcgcgatg
    16801 attatcaaaa aagagtacgg cggactggag ttctcagctt atgcccagtc tcgtgtgctg
    16861 caaaaactct ctggcgtgag cgggatcctg gcgattaccg tcggcgtgcc aaactcatta
    16921 ggcccgggcg aactgttgca acattacggc actgacgagc agaaagatca ctatctgccg
    16981 cgtctggcgc gtggtcagga gatcccctgc tttgcactga ccagcccgga agcgggttcc
    17041 gatgcgggcg cgattccgga caccgggatt gtctgcatgg gcgaatggca gggccagcag
    17101 gtgctgggga tgcgtctgac ctggaacaaa cgctacatta cgctggcacc gattgcgacc
    17161 gtgcttgggc tggcgtttaa actctccgac ccggaaaaat tactcggcgg tgcagaagat
    17221 ttaggcatta cctgtgcgct gatcccaacc accacgccgg gcgtggaaat tggtcgtcgc
    17281 cacttcccgc tgaacgtacc gttccagaac ggaccgacgc gcggtaaaga tgtcttcgtg
    17341 ccgatcgatt acatcatcgg cgggccgaaa atggccgggc aaggctggcg gatgctggtg
    17401 gagtgcctct cggtaggccg cggcatcacc ctgccttcca actcaaccgg cggcgtgaaa
    17461 tcggtagcgc tggcaacggg cgcgtattct cacattcgcc gtcagttcaa aatctctatc
    17521 ggtaagatgg aagggattga agagccgctg gcgcgtattg ccgggaacgc ttatgtaatg
    17581 gatgctgcgg catcgctgat tacctacggc attatgctcg gcgaaaaacc tgccgtgctg
    17641 tcggctatcg ttaagtatca ctgtacccac cgcgggcagc agtcgattat tgatgcgatg
    17701 gatattaccg gcggtaaagg cattatgctc gggcaaagca acttcctggc gcgtgcttac
    17761 cagggcgcac cgattgccat caccgttgaa ggggctaaca ttctgacccg cagcatgatg
    17821 atcttcggac aaggagcgat tcgttgccat ccgtacgtgc tggaagagat ggaagcggcg
    17881 aagaacaatg acgtcaacgc gttcgataaa ctgttgttca aacatatcgg tcacgtcggt
    17941 agcaacaaag ttcgcagctt ctggctgggc ctgacgcgcg gtttaaccag cagcacgcca
    18001 accggcgatg ccactaaacg ctactatcag cacctgaacc gcctgagcgc caacctcgcc
    18061 ctgctttctg atgtctcgat ggcactgctg ggcggcagcc tgaaacgtcg tgagcgtatc
    18121 tcggcacgtc tgggggatat tttaagccag ctttacctcg cctctgccgt tctgaagcgt
    18181 tatgacgacg aaggccgtaa tgaagccgat ctgccgctgg tgcactgggg cgtacaggat
    18241 gcgctgtatc aggcagaaca ggcaatggac aatttactgc aaaacttccc gaaccgcgtg
    18301 gttgccgggc tgctgaatgt ggtgatcttc ccgaccggac gtcattatct ggcaccgtct
    18361 gacaagctgg atcataaagt ggcgaagatt ttacaagtgc cgaacgccac tcgttcacgt
    18421 attggtcgcg gtcagtacct gacgccaagc gagcataacc cggttggttt actggaagag
    18481 gcgttggtgg atgtgattgc cgccgacccg attcatcagc ggatctgtaa agagctgggt
    18541 aaaaatctgc catttacccg tctggatgaa ctggcgcaca acgcgctgac gaaggggctg
    18601 attgataaag atgaagccgc tattctggtg aaagcggaag aaagccgtct gcgcagtatt
    18661 aacgttgatg actttgatcc ggaagagctg gcgacgaagc cggtaaagtt gccggagaaa
    18721 gtgcggaaag ttgaagccgc gtaattgaat aacggagccg aaaggctccg tttctttatc
    18781 cgctaattat ttaaaattaa agccatccgg atggttttcc aggctgccgg tcaacgccgc
    18841 gaacagcacc gttttaccat caatcgaaag cgcatcgttc acattcagcc aggtgagttt
    18901 ctcttgcgac gttttctcat caatagccga gaacagcccc gtcatctgat tagatttctc
    18961 ggaccacatc acagcgatac gttgcgagcc acagtcatgc ggtttgcacg cgctcatcac
    19021 ctgatacgtc tcatctccca acgttacggt ttgtgcggga gtataagtac cgcctttcat
    19081 cacccatgca ggtagcttat gcccttgcac catctgatta aacgcagctt tggtggtttc
    19141 gccctttgca aggctgctaa tggttaaatc atcctgcgcc attgcactgg tggcgatgac
    19201 cagtgcggcg actgtcgtta ttgccttaaa catcattcct cccgagctta tcctgcccat
    19261 gttattaacc tcctaaagcc tggcgataac acgataattt gcaaatcaga tatttccaat
    19321 atcagcattt gttatcaatt tattctgcgc taaggcaact gcctgataag gtgttaaagt
    19381 tataaaaccc ttcgcataaa ggagttgtac accgtgccta gtttgaagat tacgcttttg
    19441 cagcaaccgt tggtgtgggt ggatggtcct gccaacctgc gccattttga tcgtcaactg
    19501 gaaggtatta ccgggcgcga tgtgatcgtt ctaccggaga tgtttaccag cggctttgcc
    19561 atggaagcgg cagcttcgtc gcttgcacaa gatgacgtag tgaactggat gacagctaag
    19621 gcgcagcagt gcaatgcgct gattgcaggc agtgttgcat tacaaacgga gtctggttcg
    19681 gttaaccgct ttttgctggt tgagccgggc ggcacggtac atttttatga taagcgtcat
    19741 ctgttccgca tggcagatga gcatctgcat tataaagcgg gcaatgcgcg agtgattgtg
    19801 gaatggcgcg gctggcgtat tttgccgctg gtgtgctacg acttacgttt tccagtgtgg
    19861 tcgcgcaatc tcaacgatta tgacctcgcc ctgtacgtcg ccaactggcc agctccgcgc
    19921 tctctgcact ggcaggcatt gctgacggcc cgcgcgattg agaatcaggc gtatgtggcg
    19981 ggatgcaatc gcgtcggcag cgatggcaac ggctgccatt atcgcggtga cagccgggtg
    20041 attaatccgc aaggcgagat tatcgctacc gccgacgcgc ataaggcaac gcgtattgat
    20101 gcggagctgt cgatggtggc attgcgggaa tatagagaaa agtttccggc atggcgggat
    20161 gcggatgagt ttaggttgtg gtgagtgggg gttgtgttta gggccggggg ctgggctaga
    20221 ttacgaaagc ccctaactta aacacaatat tacgccagaa gtcaccattt ccaaaacaca
    20281 gcgtcgctgt accgtgggtt atctgcccaa ccgcagcgac acgccggtga tcaaaatcag
    20341 cgccaaatgg ctgagaaaag cgggatttga gatcggcacg ggcgttaccg tgaagatctc
    20401 ggagggatgt ttgatcctgc ttgggataac aacgaagtac aggaactgcg cagggaactt
    20461 tatcaggtta aacagccagt taagggga
//
LOCUS       DAEEUE010000073        20288 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.073, whole genome shotgun sequence.
ACCESSION   DAEEUE010000073 DAEEUE010000000
VERSION     DAEEUE010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20288
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.073"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(60..818)
                     /gene="aroD"
                     /locus_tag="KLK84_002792"
     CDS             complement(60..818)
                     /gene="aroD"
                     /locus_tag="KLK84_002792"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBE2010050.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(849..1715)
                     /gene="ydiB"
                     /locus_tag="KLK84_002793"
     CDS             complement(849..1715)
                     /gene="ydiB"
                     /locus_tag="KLK84_002793"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HBE2010051.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLVCEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1727..2998)
                     /locus_tag="KLK84_002794"
     CDS             complement(1727..2998)
                     /locus_tag="KLK84_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE2010052.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVQFGERKFSTRLNTIKHRG"
     gene            complement(3181..4386)
                     /locus_tag="KLK84_002795"
     CDS             complement(3181..4386)
                     /locus_tag="KLK84_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE2010053.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLHSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4486..4842)
                     /locus_tag="KLK84_002796"
     CDS             complement(4486..4842)
                     /locus_tag="KLK84_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HBE2010054.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4978..5085)
                     /gene="rprA"
                     /locus_tag="KLK84_002797"
     ncRNA           complement(4978..5085)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="KLK84_002797"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5271..6383)
                     /gene="ydiK"
                     /locus_tag="KLK84_002798"
     CDS             complement(5271..6383)
                     /gene="ydiK"
                     /locus_tag="KLK84_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HBE2010055.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6772..9828
                     /locus_tag="KLK84_002799"
     CDS             6772..9828
                     /locus_tag="KLK84_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBE2010056.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVEGIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9825..10235
                     /gene="menI"
                     /locus_tag="KLK84_002800"
     CDS             9825..10235
                     /gene="menI"
                     /locus_tag="KLK84_002800"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HBE2010057.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10335..10523
                     /locus_tag="KLK84_002801"
     CDS             10335..10523
                     /locus_tag="KLK84_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HBE2010058.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11071..11439
                     /gene="sufA"
                     /locus_tag="KLK84_002802"
     CDS             11071..11439
                     /gene="sufA"
                     /locus_tag="KLK84_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HBE2010059.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11448..12935
                     /gene="sufB"
                     /locus_tag="KLK84_002803"
     CDS             11448..12935
                     /gene="sufB"
                     /locus_tag="KLK84_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBE2010060.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12945..13691
                     /gene="sufC"
                     /locus_tag="KLK84_002804"
     CDS             12945..13691
                     /gene="sufC"
                     /locus_tag="KLK84_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBE2010061.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13666..14937
                     /gene="sufD"
                     /locus_tag="KLK84_002805"
     CDS             13666..14937
                     /gene="sufD"
                     /locus_tag="KLK84_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBE2010062.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDSLALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14934..16154
                     /gene="sufS"
                     /locus_tag="KLK84_002806"
     CDS             14934..16154
                     /gene="sufS"
                     /locus_tag="KLK84_002806"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HBE2010063.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLIAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16167..16583
                     /gene="sufE"
                     /locus_tag="KLK84_002807"
     CDS             16167..16583
                     /gene="sufE"
                     /locus_tag="KLK84_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HBE2010064.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16732..17736
                     /gene="ldtE"
                     /locus_tag="KLK84_002808"
     CDS             16732..17736
                     /gene="ldtE"
                     /locus_tag="KLK84_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HBE2010065.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRIAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17799..18035)
                     /gene="lpp"
                     /locus_tag="KLK84_002809"
     CDS             complement(17799..18035)
                     /gene="lpp"
                     /locus_tag="KLK84_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HBE2010066.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18346..19758)
                     /gene="pykF"
                     /locus_tag="KLK84_002810"
     CDS             complement(18346..19758)
                     /gene="pykF"
                     /locus_tag="KLK84_002810"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HBE2010067.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5317 a         4854 c         5175 g         4942 t
ORIGIN      
        1 ggttaaaaat acttttttta tttcggcggg ggagggtatt tccgccgaaa tattattgct
       61 tatgcctgat gtaaaatagt taataccgtg cgcaaatcat ttaccgagat ttgccctggc
      121 gcagaggctt tttttaccgc accaaaagtt gccgccgagc caaatacttc accaaccaga
      181 cgagaaatta cgccagtttt tgccatcgac atcgtgatga ttggacgatc ggcatactgc
      241 tcctgcatct ccagggtcgc ggcaagcaac gtcagcacat cgctggtact ttgcggcatc
      301 agcgcaatct taggaatatc ggcgtcgaag gactgcattt tgcgcagacg ggcaatgatt
      361 tcttcggctt ccggcgtttt atggaagtca tggttggaca tgacaacttt cacatcatgc
      421 gcgtgggcgt aggcgacggt ttctttgacc tgatcatcgc cggtaaataa ctccagatcg
      481 atcatatcaa ccaggccgct gtcgatggct gcacgattga gagcaatata agcctcggtg
      541 gaaatcgcct gctcgccgcc ttctttggca ctgcggaagg taaacagcag cggtttttct
      601 ggcatggttt cacggagaat ttttgccgcc gccatgacag actccacatt ggagaggtcg
      661 gcaaagtggt ccacacgcca ttccagaata tcaaagtccg cttcacgata ggcgagagct
      721 tcggatttca cgcgggcgat atctttcgcc atcagcgaga cgatgatttt aggtgcgccc
      781 gcaccaatga cgagatcttt tacagttacg gttttcattt tttacccttt ctgcacgcgg
      841 tcagcctgtc aggcaccgaa ccccatgacc tgtttaacat attccagagg gaaatctttg
      901 ccagtccata acgtgaactg ttcagcccct tgccacaaca acatgccgta tccatcaatc
      961 gttttgcaac cagcttgttg tgcctgctgc aataacttcg tcatatgcgg gttatacacg
     1021 cattcagtga ccagcagtcc cggatgtaac agactgatat cattaaccaa tgattcattc
     1081 tcaaggggtt tcatacctac ttttgtgcca ttggttaaaa tgtcggcgga agccagggct
     1141 tcagcaaagg cttgctgatc ggcgagatcg gtatccgtga cgacacaatc ggtgttttcg
     1201 ttaacccgct gcgcgaaggc gagggcttta tcgaagaact catcccgacg gttaaagagt
     1261 ttaatttctt ttaaaccttc aattgccccc tgcgcgccaa tcgccgttga ggcacctccg
     1321 gcccctaaca gcaccatcgt tttgcctttg atatcaaaac cgctctcttt aatggcgcga
     1381 atatggcccg tgccgtcggt gttatagcca cgcagatagc catcatcatt aacgatggtg
     1441 ttgatggcac ccaccagttt ggctgctggc gttaattcat caacatattc acacaccagt
     1501 tgtttgttcg gcatcgacac accagttccg cgcattttga gggcttttaa tccttcaatt
     1561 gctccgggaa agctatcgtt atccacttcg aaggccatat aggtaaatgg caatcccgct
     1621 ttttctaagg ctttattctg catttcaggc gataaactgt ggcggatagg ataagccatc
     1681 aatccaatca attcgtattt tgcggtaaca tccataatta actcctttaa cctctatgct
     1741 taattgtgtt taaccgggtg ctaaatttgc gttcgccaaa ctgcacatca ttctcaggaa
     1801 taataaaaac gcggtaatag cggctaaaaa caataattgc ggtaatcagg gccagcagcg
     1861 cgaaagtaaa atcgagaaca atgatatatt gcaggccgat gttcgacaga taaccggtaa
     1921 tcagcggaat aacaaagtta gccagtccac ccatcatcat ataaatactg gtgactttgg
     1981 ctttgctttt ggggaaaaac tctgacatga ccgaaacacc gagctgtaaa atgccgccag
     2041 ctgctgagaa accgataaca aaggcgccgg cattacacac cagtggagaa gggtacaggt
     2101 agataatggc agctgttatt gttgccagcg cagagttaaa tacattagcc cagatgggcc
     2161 ggaccatttt tttcagtagt gcggcaaaaa taaagacaca gaccaacgag cccatactgt
     2221 aataagagat ggtttttaat gcctcagctt ctgacatacc agcaaaagcc atcgcatatt
     2281 tgggcatcca caccacaatc acataaaagg tcgagaatgc ggctacaccg aacagtaccg
     2341 atgaaacacc ttccagccag actaaaggtt tgctgttcat ttgcggtaat tcattagcta
     2401 cgctggcgtc caccaactgg ctggggaatt tgcttttcaa cagcatcagc gtgatcagta
     2461 caaacagaat acccggaata atcagcccat agccgtacca gatattattg agcaacatat
     2521 agctcaccag cattgggtag aacatttgcc caaatgacac catcgcttta accagtatga
     2581 ccgccgaacc agaggctttc ggaaagcatt ccatgagcgc ggggtagcca cccgtatcca
     2641 gcgctgagtt agcgatacct acgcacactg ccagaccgta ggcgagagtt aaattcgggc
     2701 aagcgggaat accaaagaag aatagcagat acattattac tgccattaat atcaccgccc
     2761 gacgaccaaa cttatcggag atcacaccga agaataaaat actgatcaat cgccccaaac
     2821 cgataccgga aattaaatag gcaatgcccg cgttgtcagt ggaaaacttt tctgccagag
     2881 atgtcatatt ttgggcaagc gtaataacac taatgccgtg caggaaatag ctgaagtaaa
     2941 tacaaagaac agccaggata aatggcgtgc tgaaagataa tttcgttacc gtacgtattg
     3001 ttctccgacg gtttacataa ttactatact gaagatgatt ttccctgggt acatggcata
     3061 aagtttatgt ttccaggaat attgcatgtc atagtttgtt tttgatcgcg attttcctta
     3121 gttagcgcag aaagagttat ttatccgtaa gatcttgcat gacctgaaaa gaatattgca
     3181 ttacccaccc ggagcgacat tttcctttag cgagtgatgc cgcgtttttt tgcggctgtg
     3241 taagccgata aacagtgcca gtaaaaaacc gatggcagta tcgaaccaca taatatcggc
     3301 aatacttctt tgggacagat gagccgtaat caacggaata gtaaaggtcg caatactgcc
     3361 cgtactgtaa tagatccctg tagctttacc tttagcgtaa gggaaacgtt cagccattaa
     3421 cgtcaggcca atttgcacaa cacctccagc agaggtaaaa ccaatgacaa aagcaaatat
     3481 tatcaccaca taaaatgtgg gatgcaggca gacggtaaac agagcaataa atgagataaa
     3541 ggtgtacagc atcagtaatg ttgttgggcg aacggtatta cgaatgagtg gagcggtaat
     3601 aaatacacaa agcagcgaac ccacggtgta gatactgagt agtttgatcg acatagtgta
     3661 tgacatgcct gcaacaaatt gtccgtactg tgccagccac tggctaacca gataaaacgt
     3721 tgccattgag atatagccat ataaggtata actggctaaa tcgataattg aacagcgatg
     3781 ttccgtagag ctggtggttt tctttatgac aggtaagcga cgacccggat ggggtgggaa
     3841 cgtacaacgg tataaaaaca gagcgttaat aaacataatg cctgcagcaa tcataaagga
     3901 ccaaccgaac cacagttcag cccacactaa caggctaatg attagcggta ataaaaattg
     3961 tccgctggaa acaaatgctt taattaaaat attggctgtc ccaggtgagc gtggaaaagc
     4021 ttccatcaaa ctgggataag tgcctgcatc gagaaaactg tttgccattc ccgccagaaa
     4081 gccaaaaaca taagcgataa tgatgttatt ggtctgcagg atgtcaaaaa agaaggccat
     4141 atagcagcac atcccgagca tgataaaagg gcggcgacca aagcgatcgg ataataatcc
     4201 tgcaaaaagc aagacactta atcgaccaat gcccagcgat gagataacta tcgagacacc
     4261 cgcggcatta gtctgccaaa gtgtctccag cgaggccata ttcaggctca tcaaaatgac
     4321 gcccatacca tgcaccaggt aattaaaata caacccaagt gcggtagggt aatagggatt
     4381 tttcataggt tctacttagc aaaggtgctt taaaaagtgt agcgatgttg tgtcagtatc
     4441 cattcattat tgttgtaaca acgacaattc ctttctgtgg aaaaattaac aaagccgctc
     4501 cagatcgtgt gcatataatt cagcggctac cgattgatag atcttccagt caataaaatt
     4561 gccatcaggg tagacttgtt gaaacgcggt taagtcgggg aaaaaacgca tggtgttgtt
     4621 gccgatgcgg ttatttattt tctctatgat ggcatgaaga tgtttttttc gttgctgacg
     4681 catagccaac agttgctcga ctacacaatc aggaatggcg catttgccat tttcccactg
     4741 acgccagctt tcactgtcac cggtctgggc aatccaggtc gcgcattcat caatagtcat
     4801 ggcaaaaata tggcggagtg cctggagttc ataagcgttc atcgtgtaat ggctgcatga
     4861 aataaacaat aaaatataaa taacattatc agcctgctga cggcttgaag agagtcacag
     4921 tatcttgtgc aacattattg gaaagagtga ggggcgaggt agcgaagcgg aaaaatgtta
     4981 aaaaaaagcc catcgtggga gatgggaaaa gactacacac agcaattcgt tgtttcactc
     5041 aggggatttc catgcttata aatcaatatg ttgatttata accgtgagct aatagtaggc
     5101 atacggactt tttgcgtcga tcagattcgt ctcattagtt taaaaactgt aaagaatttg
     5161 cgacaggtac taccagctga atggcggtaa gcaggaaaat atggggaaga gcaagcaaat
     5221 atcaattagc ggtgattaac aatcaggcgg cagagccgcc tgatgagaaa ttacttattc
     5281 ggtttctcga tttcgccaag ttcttcgaga atttcttccg gttggtccgt cggtggcggg
     5341 acttcttcca cccacgcggc aaacagacgc caggaaacgg ctaacagaac cggaccaata
     5401 aacagaccga tcatcccgaa agcaatcaaa ccaccaataa cgccagagag gatcaggatc
     5461 agcggtaaat cggcacccat gcgaattaac attgggcgga tgacgttatc cagcgtgcca
     5521 accacaccgc tccacactaa caataccgtt ccccaggtgg tatcgccggt ccagtagagc
     5581 cagataatcg ccggaatcag taccggcaac gggccaagct ggacaaggca ggagaggatc
     5641 attaacaccg ttagcaacgt tgcataaggt acgccggata cggcgaggcc gataccgcca
     5701 agcactgcct gtactaacgc cgtcaccacc acacccagcg ccaccgcgcg gatagcctgt
     5761 gccgccagca gcacggcggc atctccgcga acgcctgcca gacgggttgc aaaatggcga
     5821 atgccttgtg ccacctgttc accgcgccag tacagcaggg cactgaagag aagcatcagc
     5881 gcacaatgca ccataaagcg cccgatatgc gccgcctgcc caacgaacca ggtggtggtg
     5941 gtgccaatat aagggcggac tttcgccatg atcgccgtac cccccatatc cagcaagttg
     6001 tgccagcctg catacagctt cgcgccaatc accggaatgg tattaagcca cgccagatcg
     6061 ggtaacgtca tgtcgccgct ggaaatggct ttaattagcg ggccgctgcc gtcgacgata
     6121 ctgttaacca gcaaagcaat agggatgata aacaccatca ctaataacag cgtcatcacc
     6181 agaacggcga gagagcggcg gccaaacatg atcttttgca aacgtaacaa taccggccag
     6241 gtggcgataa ccaccgtacc ggcccatgca aagccgagaa taaagggttg aacaatccac
     6301 agacatgcca caatcatgat ggctaaaaac agcaccgaaa gcagaatttg tgcgacatcc
     6361 ctgggctgac gaacatttac cataactact tttcaccttt tttgtgcgtc aaatcgtcga
     6421 cgcgacgtga acacgcgaaa ctcacctgca taatgatgag caatttcagc ggtttttaac
     6481 aggccgattc tgcccttaat tctgatgggc gtgttagaaa aaaatgtggt acaacaactc
     6541 aggcaacacg caaacgattt actcgtcgta tttcaagttg catgtgctgc ggctacgttc
     6601 gttcacccca gtcacttact attgtaagct cctggagttc actcgcttgc cgccttcctg
     6661 caactcgaaa tccatagagt aaataaacat cacctttcag tgcgctattg cagccgcctg
     6721 aaagaatagt gtatcgacct acatcacaga ccgcaggaaa gggtcaatat aatgattcca
     6781 cagatttccc tggcacccgg tgtcgttcaa ctggtgctta attttttgca agagctggag
     6841 caacaaggtt ttaccggcga tgcggcgaca agttatgccg atcgtctgac aatgtcgacc
     6901 gacaacagta tttaccaact tctccccgat gcggtggtat ttccgcgttc aaccgcagat
     6961 gtggcgctga tcgcccgtct tgccgcgcag gaacgctatt catcgctgat ctttaccccc
     7021 cgcggcggcg gcaccggcac taacggtcag gcgctcaacc aggggattat tgttgatatg
     7081 tcccgccata tgaaccgcat catcgaaatt aaccctgaag agggctgggt gcgcgttgag
     7141 gccggggtga taaaagacca actcaatcag tacctgaaac cgttcggcta cttttttgcg
     7201 ccggaacttt cgaccagcaa ccgggcaacg ctcggtggga tgatcaatac cgatgcatcc
     7261 ggtcagggat cgctggtcta tggcaaaacg tcagatcacg tacttggcgt acgcgcggtg
     7321 ttgttggggg gcgatattct cgatacgcaa cctttacccg tcgagctggc ggaaacgctg
     7381 ggtaaatcca ataccacaat cgggcgaatt tataacacgg tttatcaacg ttgccgtcag
     7441 caacgccagt taatcatcga caacttcccc aaacttaacc gctttcttac cggttacgat
     7501 ctgcgtcatg tctttaacga tgagatgacc gagttcgacc tgacgcgcat tctgacgggt
     7561 tcagaaggga cgctggcctt tattaccgaa gcgcggctgg atattacgcg cttgcctaaa
     7621 gtgcgccgtc tggtgaatgt caaatatgac tcttttgact ctgcgctgcg taacgcgccg
     7681 tttatggttg aggcgcgggc gctttcggta gagacggtgg actcaaaagt gctgaatctg
     7741 gcgcgggaag atattgtctg gcattccgtc agcgagttga ttaccgatgt gcctgacaaa
     7801 gagatgctcg gactgaacat tgtggaattt gctggtgatg atgaggcgct gattgacgag
     7861 cgagttaatg cactctgtgt gcggcttgat gagctgattg ccagccaaca ggcaggtgtg
     7921 attggctggc aggtgtgccg cgagctggcg ggggttgaag gtatctatgc gatgcgcaaa
     7981 aaagccgttg gtctgcttgg caatgccaaa ggtgccgcta agccaattcc gtttgctgag
     8041 gatacctgcg taccgccgga acatctggcc gattatattg ctgaatttcg cgcgctgctc
     8101 gacagccacg gcttaagcta cggtatgttc ggtcacgtcg acgcaggtgt cttgcacgtc
     8161 cgtccggcac tggatatgtg cgatccgcaa caagagattt tgatgaagca aatctctgat
     8221 gacgtggtgg cgctgactgc gaaatacggt ggtttgttgt ggggcgagca cggcaaaggt
     8281 tttcgcgctg aatacagccc ggcgtttttc ggtgaggaac tttttgcaga actgcgcaaa
     8341 gtgaaagcgg catttgaccc gcataaccga ctcaacccag ggaagatttg cccgccagaa
     8401 ggtctcgatg cgccgatgat gaaagtggac gcggtgaagc gcggtacatt cgatcggcag
     8461 atccccattg cggtacgcca gcagtggcgc ggtgcgatgg agtgtaacgg caacggttta
     8521 tgcttcaact ttgatgcccg tagtccgatg tgtccgtcga tgaagatcac ccagaaccgg
     8581 attcattcac cgaaagggcg cgcaacgctg gtgcgtgaat ggctgcgctt gttggcggat
     8641 cgcggcgttg acccactcaa actggaacaa gaactgcctg aatccggcgt cagtttgcgg
     8701 acgttaattg cccgcacgcg caatagctgg catgcgaata aaggcgaata tgacttctca
     8761 cacgaagtca aagaggcgat gtcgggctgt ctggcctgta aagcttgttc gacccagtgc
     8821 cccatcaaaa ttgatgtgcc ggagtttcgc tctcgttttc tgcagctcta tcacacccgt
     8881 tatttacgcc cgcttcgcga ccacctcgtt gctacggtcg agagctacgc accgctgatg
     8941 gcacgcgcgc cgaaaacctt taacttcttc attaaccagc cgctggtgcg caaactctcg
     9001 gaaaaacata tcggcatggt tgatttgcca ctgctgtcgg tcccctcgct acaacaacaa
     9061 atggtggggc atcgctcggc aaacatgacg ctggaacagc ttgaagctct caatgcagag
     9121 cagaaagcgc gcacggtgtt ggtggtgcag gaccccttta ccagctatta cgatgcgcaa
     9181 gtggtggcgg attttgtccg tctggttgaa aaattaggtt tccagcctgt gttactgcca
     9241 ttttcgccaa atggcaaagc ccagcatatc aaaggctttc ttaatcgttt tgcgaagacg
     9301 gcgaaaaaga cggcggattt cctcaaccgt atggctaagc ttgggatgcc aatggtgggc
     9361 gtcgatccgg cgctggtact ttgttatcgc gatgaatata aactggcgct gggcgaggaa
     9421 cgtggcgagt ttaacgtctt acttgcgaat gaatggctgg caagcgcact tgagtcacat
     9481 ccagtggcta cagtcagcgg tgaatcatgg tatttctttg gtcactgtac cgaggttacc
     9541 gctttgccgg gcgcgccagc acaatgggcc gcgatatttg cccgttttgg cgcgaaactg
     9601 gaaaatgtca gcgtgggttg ctgcggcatg gcagggactt acggacatga agcgaaaaac
     9661 catgaaaatt cgctcgggat ctatgagtta tcctggcatc aggctatgca gcgactgccg
     9721 cgtaaccgct gtctggcgac cggatattcc tgccgtagcc aggtaaaacg ggttgaaggc
     9781 acgggggtac gccatcctgt gcaggcttta ctggagatta ttaaatgata tggaaacgga
     9841 aaatcaccct ggaagcactg aatgctatgg gtgaaggaaa catggtgggg ttcctggata
     9901 ttcgctttga acatattggt gatgacaccc ttgaagcgac aatgccagta gactcgcgga
     9961 caaagcagcc tttcgggttg ctgcatggag gagcatccgt ggtactggcc gaaagtatcg
    10021 gttccgttgc cggttattta tgtaccgaag gtgagcaaaa agtggttggt ctggaaatca
    10081 atgctaatca cgtccgctcg gcacgagaag ggcgggtgcg cggcatatgt aaaccgttgc
    10141 atctcggttc gcgtcaccag gtctggcaga ttgaaatctt cgatgagaaa gggcgtttgt
    10201 gctgttcgtc acgattgacg actgccattt tgtgacgttg tcaccagaaa agtgtgacgc
    10261 atattgcttt tggtcattat ctgcacagta aacggtggta tactgtgcag gtcttgtcta
    10321 aaagcgagga tgctatgtct actcaacttg atcccaccca gctggccatt gaatttttac
    10381 gccgtgacca gagcaatctt tcacctgccc aatatctcaa acgtctgaaa caactagaac
    10441 ttgaatttgc tgacctgctt actctctctt ctgctgaatt aaaagaagaa atctactttg
    10501 cctggcgtct gggcgtgcat tgatcctgac ttaatgccgc aagttcgcca aatcgacctg
    10561 gattaacctc cgtccaggtt cgatgacctt cttaatactg cttatctacc gcctgatatt
    10621 gttcaaaaat aatactaatc gcagtttgtg ttaaaacggc gggttagctt tatgagatta
    10681 ttcttatcgc cccttcaaga gctaagccac tgagagtgcc ggagataagc gccggatggg
    10741 gtagaaaccc ttaagcctgt gtcgcacaga cttaagggtt ttcttatttc tgaacgctgc
    10801 acttttttac ctatctctta aatagtcatt ttcaatacaa ctttttcttt ttcatcacag
    10861 ccttaatgct ccttagataa taaccattat ctaacaatga gatacctaat tcttagcgtg
    10921 cctgttaacc cacacatcag gggctatgct tattaaaagc aggcaaaaag aatgttttac
    10981 ggtaaagccc ctgcgtttgc tgggttgaac tgataatcat tatcactaac atgctgttat
    11041 acgctgaaag cgatgaagtg aggtaaatag atggacatgc attcaggaac ctttaaccca
    11101 caggatttcg cctggcaagg cttaacgctg acacccgcag cggcgataca catccgcgag
    11161 ctggtggcaa agcagcctgg tatggtcggc gtgcgcttag gtgtgaagca aacgggctgc
    11221 gcgggctttg gctatgtgct cgacagtgtt agcgagccgg acaaagacga tctgctgttt
    11281 gaacacgacg gcgcgaagct gtttgtcccg ctgcaatcga tgccgtttat tgatggcacg
    11341 gaagtcgatt tcgttcgtga aggacttaat cagatattca aatttcacaa ccctaaagcc
    11401 cagaatgaat gtggctgtgg cgaaagcttt agggtatagg cggtactatg tctcgtaata
    11461 ctgaagcaac tgacgatgtc aaaacctgga ccggcggccc gctgaattat aaagaaggat
    11521 tcttcaccca gttagccacc gatgagctgg caaaggggat aaacgaagag gtggtgcgcg
    11581 caatttcggc gaagcgtaat gagccggagt ggatgctgga gtttcgtcta aacgcctatc
    11641 gcgcatggct ggagatggaa gaaccgcact ggttgaaagc gcactacgac aagctgaatt
    11701 atcaggatta cagctactac tcagcaccat cgtgcggtaa ttgtgacgac acttgcgcgt
    11761 ctgaacctgg cgcggtgcag caaactggcg cgaacgcctt tttaagtaaa gaggtggagg
    11821 cggcgtttga gcagttgggc gttcccgtgc gggaaggcaa agaggtggcg gtggatgcca
    11881 ttttcgactc tgtttcggtt gccaccactt atcgcgaaaa actggcggag cagggaatta
    11941 ttttctgttc ctttggtgag gcgatccacg atcacccgga actggtgcgt aaatatctcg
    12001 gcaccgtggt gccggggaat gacaacttct ttgcagcact taatgcggcg gtagcctctg
    12061 acggtacgtt tatttatgtg cctaaaggcg tgcgctgccc gatggaactt tccacctatt
    12121 ttcgcattaa cgcggaaaaa accgggcagt ttgagcgcac aattctggtg gccgacgaag
    12181 acagctacgt cagctacatt gaaggctgtt ccgctccggt gcgtgacagc tatcagttgc
    12241 acgcggcagt ggtggaagtc atcatccata aaaacgccga ggtgaaatat tccacggtac
    12301 aaaactggtt ccctggcgat aacaacaccg gcggtattct caacttcgtc accaagcgtg
    12361 ctttgtgcga aggcgaaaac agcaaaatgt catggacgca atcagaaacc gggtcagcga
    12421 ttacgtggaa atatcccagc tgcattttgc gcggcgataa ctccattggt gagttttact
    12481 cagtggcgct gaccagcggt catcagcaag cggataccgg caccaagatg atccacatcg
    12541 gtaaaaacac caaatcgacc attatctcga aagggatctc tgccggacat agtcagaaca
    12601 gttatcgcgg cttagtgaaa atcatgccga cggcaaccaa tgcgcgcaat ttcactcagt
    12661 gcgactcaat gctgattggc gctaattgtg gggcgcatac cttcccgtat gtcgaatgtc
    12721 gcaataacag cgcacaactg gagcacgagg caacgacatc acgtattggt gaagatcaac
    12781 tgttttactg cctgcaacgc gggatcagcg aagaagacgc catctcgatg attgttaacg
    12841 gtttctgcaa agacgtgttc tcagaactgc cgctggaatt tgccgttgaa gcacaaaaac
    12901 tcctcgccat cagtcttgaa cacagcgtcg gataaggaat aaacatgtta agtattaaag
    12961 atttacacgt cagcgtggaa gataaagcta tcctgcgtgg attaagcctc gacgttcgtc
    13021 ccggcgaagt tcacgccatt atggggccaa acggttcggg caaaagtacc ttatcggcaa
    13081 cgcttgccgg gcgcgaagat tatgaagtga cgggcggcac ggttgagttc aaaggcaaag
    13141 atttgcttgc actgtcgccg gaagatcgtg caggcgaagg catctttatg gccttccagt
    13201 atccggtgga gattccaggc gtcagtaacc agtttttcct gcaaacggca cttaatgcag
    13261 tgcgcagcta tcgcggccag gaaacgctcg accgctttga ttttcaggat ttgatggaag
    13321 agaaaatcgc tctcctgaag atgccggaag atttattaac ccgttcggta aacgttggtt
    13381 tttccggcgg cgagaaaaag cgcaacgata ttttgcaaat ggcggtgctg gaaccggagt
    13441 tatgcattct tgatgagtcg gactccgggc tggatattga cgcattaaaa gtggtcgccg
    13501 atggcgtgaa ctcgctgcgt gatggcaagc gctcattcat cattgttacg cactaccaac
    13561 gcattctcga ctacatcaag cctgattacg ttcatgtgct atatcaggga cgaattgtga
    13621 aatccggcga tttcacgttg gtcaaacaac tggaggagca gggttatggc tggcttaccg
    13681 aacagcagta atgcgctgca acagtggcat cacttgtttg aagctgaagg gacaaaacgc
    13741 tcaccgcaag cacagcagca tttacaacaa ttgctgcgta ccggactgcc gacacgtaaa
    13801 catgaaaact ggaaatatac gccgctggaa gggctgacca atagtcagtt tgtcagcatt
    13861 gcgggagata tatccccaca gcagcgtgat tccttagcgt taacgttaga cgccgtgcgg
    13921 ctggtgtttg tcgatggacg ttacgtgccc gcgctgagcg atgcaattga aggcagcgga
    13981 tatgaagtga gcattaacga cgaccgtcag gggttacccg acgctattca ggcggaagtg
    14041 tttctgcatt tgacggaaag cctggcacaa agcgtgacgc atatcgccgt gaagcgcggt
    14101 caacggccgg caaagccatt gctgttaatg catatcaccc agggcgtggc aggtgaagag
    14161 gtgaacactg cccattaccg gcatcatctg gatctggcgg aaggtgccga agcaacggtg
    14221 atcgaacatt ttgtcagcct taatgatgct cgtcatttta ccggggcacg gttcactatc
    14281 aatgtcgcag cgaacgccca cttgcagcat atcaagctgg cgtttgaaaa cccggtcagt
    14341 caccactttg cccataacga tttgttgctg gctgacgatg ccaccgcatt tagccatagt
    14401 ttcctgctgg gtggcgcagt gttacgacat aacaccagta cgcaactcaa tggcgaaaac
    14461 agcacgctgc ggatcaatag cctggcgatg ccggtgaaaa acgaggtgtg tgatacccgt
    14521 acctggctgg aacacaataa aggtttttgt aacagccgac agttgcacaa aactatcgtc
    14581 agcgacaaag gccgcgcggt atttaacggt ttgatcaacg tcgcgcagca cgccatcaaa
    14641 acggatggtc agatgaccaa caataatctg ctgatgggca aactggcgga agtggataca
    14701 aaaccgcagc tggaaatcta tgcagatgat gtgaaatgca gccacggcgc gacggtgggg
    14761 cgtattgatg atgaacagat gttctatctg cgctcgcgcg ggatcaatca gcaggatgcc
    14821 cagcagatga tcatttacgc ctttgctgcc gaactgacgg aagcactgcg tgatgagggg
    14881 cttaaacagc aggtgctggc ccgaatcggt caacggctgc caggaggtgc aagatgactt
    14941 tttccgtcga caaagtgcgg gccgactttc cggtgctttc gcgtgaggta aacggtttgc
    15001 cgctggctta tctcgacagc gccgccagtg cacagaaacc gagccaggtg attgacgccg
    15061 aggccgagtt ttatcgtcat ggctacgcgg cggtgcatcg cggtattcat accttaagcg
    15121 cccaggcgac cgagaaaatg gagaacgtgc gcaagcgggc atcgctgttt attaatgccc
    15181 gttcggcgga agagctggtg ttcgtccgcg gcacgacgga agggatcaat ctggtcgcca
    15241 atagctgggg caacagcaat gtgcgggcgg gcgataacat catcatcagt cagatggagc
    15301 accacgctaa cattgtcccc tggcagatgc tttgcgcacg cgttggcgca gagctgcgtg
    15361 tgatccctct caaccccgac ggtacgctgc aactggagac gctgcctacg ctgtttgatg
    15421 agaaaactcg cctgctggca attactcatg tctccaacgt gcttggcaca gaaaatccac
    15481 tggcggaaat gatcacgctt gcgcaccagc atggcgcaaa agtgctggtg gatggcgctc
    15541 aggcggtgat gcatcatccg gtggatgttc aggcgctgga ttgcgacttt tacgtgttct
    15601 ccgggcataa actgtatggc cccaccggaa ttggcattct ttatgtgaaa gaagccttgt
    15661 tgcaggagat gccgccgtgg gaagggggcg gttctatgat cgccaccgtc agcctgagtg
    15721 aaggcactac ctggaccaaa gcaccatggc ggtttgaagc cggtacaccc aataccgggg
    15781 gcatcattgg tcttggcgcg gcgctggagt atgtttcggc gctggggctt aatagcatag
    15841 ccgagtatga acagaatctg atgcattatg cgctatcaca gctggaatct gtaccggatc
    15901 tcactctcta tggccaacaa aacaggcttg gcgttattgc ttttaatctc ggtaaacacc
    15961 acgcctatga tgttggcagt tttctcgata attacggcat tgctgtgcgt accggacatc
    16021 actgcgctat gccattaata gcctattaca acgtccctgc gatgtgtcgg gcgtcgctgg
    16081 ccatgtataa cacccatgaa gaagtggagc gtctggtgac cggcctgcaa cgtattcacc
    16141 gtctgctggg ataacaggga ggcactatgg ctttattgcc ggataaagaa aaattgctgc
    16201 gtaatttttt acgctgcgcc aactgggaag agaaatatct ctacattatt gagctgggcc
    16261 agcgtctgcc agaattacgc gccgaagacg gaagcccaca aaatagcatt cagggctgcc
    16321 agagtcaggt gtggattgtc atgcgccaga atgcgcaggg aattattgaa ttgcagggcg
    16381 acagcgatgc ggcgattgtg aaagggctta ttgcggtcgt ctttattctc tacgatcaga
    16441 tgacgccgca ggatattgtc aatttcgatg tgcgtccgtg gtttgaaaaa atggcgctca
    16501 cccaacatct caccccatct cgttcacaag gtctggaagc gatgattcgc gcaattcgcg
    16561 ccaaagccgc tgcacttagc taaactaaag ggacagcttt catccggttg gctcacattg
    16621 ccggatggtc aatgtttaca ctgacctgca caggagtgtt ttggtttcag gtgaacataa
    16681 gcaccatccg acgtttcagc gtgagtctcc ggcaaataca ggaggtttac aatgaaacgc
    16741 gcgtctttgc ttacactcac gcttatcggc gcttttagcg ccatccaggc tgcctgggcg
    16801 gttgattatc cgctaccacc aaccggaagc cgactggttg ggcaaaatca aacgtatacg
    16861 gtgcaagaag gggataaaaa ccttcaggct attgcccgac gttttgatac tgcagcaatg
    16921 ttgatccttg aagccaataa cactatcgcc ccggtgccaa aacctggtac gacgataact
    16981 attccttcac aactgttatt acctgatgca ccgcgtcagg ggattatcgt taaccttgca
    17041 gagctgcgcc tttattatta tccgccggga gaaaatattg tgcaggttta tccaataggt
    17101 attggattgc aggggctgga aacgccggtg atggaaacgc gtgttgggca gaaaatccct
    17161 aacccaacct ggacgcctac ggcaggcatt cgtcagcgtt cgctggagcg tggcattaaa
    17221 ttaccgccag tcgttcctgc cggaccaaat aacccgctag gacgttacgc actgcgcatc
    17281 gcgcatggta atggcgaata cctcattcat ggcaccagtg cgccggacag cgttggtttg
    17341 cgcgtcagtt cagggtgtat tcgcatgaat gcaccggata ttaaagcctt gttctccagc
    17401 gtgcggacgg gaacgccggt gaaagtgatc aacgaaccgg tgaaatattc cgtggaacct
    17461 aacgggatgc gttatgttga agtacatcga ccactatcag cagaagaaca gcaaaacgtt
    17521 cagacaatgc catacacact gccagcaggc tttacgcaat ttaaagacaa taaggctgta
    17581 gatcagaagt tagtcgataa agcgttgtat cgtcgggcag ggtatccggt ttcggtgagc
    17641 agtggagcaa ctcccgcagc cagcaatgcg ccttcagtag agtcagcgca gaatggtgaa
    17701 ccagagcaag ggaatatgtt acgcgcgacg cagtagtggt aaatagcaga caaaaaaatg
    17761 gcgcacaatg tgcgccattt ttcacttcac aggtactatt acttgcggta tttagtagcc
    17821 atgttgtcca gacgctggtt agcacgagct gcgtcatctt tagcagcctg aacgtcggaa
    17881 cgcattgcgt tcacgtcgtt gctcagctgg tcaactttag cgttcagagt ctgaacgtca
    17941 gaagacagct gatcgatttt agcgttgctg gagcaacctg ccagcagagt agaacccagg
    18001 attaccgcgc ccagtaccag tttagtagct ttcattatta ataccctcta gattgagtta
    18061 atctccatgt agcgttacaa gtattacaca aagtttttta tgttgagaat atttttttga
    18121 tgggaaggca cttatttttg atcgttcgct caaagaagca tcgaacgctg ggttttagat
    18181 aaaaaaattg agagaaaaca ggatgcttcc atcggattca tcttaaataa agtgagatta
    18241 tatagtgaaa tccgatttga ttttttattg cttctggtta tcgattaaat aaaaaaagcg
    18301 cccatcaggg cgcttcgata tacaaattaa ttcacaaaag caatattaca ggacgtgaac
    18361 agatgcggtg ttagtagtgc cgctcggtac cagtgcacca gaaaccataa ctacgacgtc
    18421 acctttgtgt gccagaccgc tctgcagagc cagttcttta cccagacggt agaaatcatc
    18481 agtagaagtg atctctttaa ccagctgcgg cacaacgcct ttgctcagta ccaactgatg
    18541 agccgttttt tcgttggtgg tcagtgccag gatggtggca tccgggaagt atttacgtac
    18601 tgcgcgagca gatttaccgc cctgggtagc aaccacgatc agcggagcat ccagtttttc
    18661 agcagtttca acggcaccac ggcataccgc ttcggtgatg cgcagtttac ggttgtcatt
    18721 gttgaactcg agacggctgt tcatcacgcg gtcggtacgt tcgcagatgg tcgccatgat
    18781 agaaaccgct tccagcgggt atttaccttt tgcggattca ccagacagca tcactgcgtc
    18841 agtaccgtcg aggatggcgt ttgcaacgtc accggcttct gcgcgagtcg ggcgtgggtt
    18901 tttgatcatg gaatccagca tctgggtcgc agtgataacg actttacgtg cacggataca
    18961 tttttcgatc atcatcttct gggcgaagat aacttcttct accgggattt ctacacccag
    19021 gtcgccacgc gcaaccatga tgccgtcaga ggcttcgagg atttcgtcga agttgttgag
    19081 gccttcctgg ttttcgattt tggagatgat gtggatgttt tcgccgccgt gcgctttcag
    19141 gtgctcacgg atttcgataa cgtcagaacg cttacgaata aaggaagcag caacaaagtc
    19201 tacgcctttt tcgcaaccaa agatcaggtc ctgtttgtct ttttcagcca gtgctggcag
    19261 agcaatggaa acgccaggca ggttcacacc tttgttttcg cccaggtcac cgttgttcag
    19321 cactttacag ataactttgt taccttcaat ggcggtaact tccataccga tcagaccatc
    19381 gtcaaccagt acggtgttgc caacagacag gtcagtagtg aaaccttcat acgttaccgc
    19441 aaccatttcg ctgttgccga taacagattt atcagtggtg aaagtaaagg tctgaccagc
    19501 tttcagagaa acatcgttac cgccttccag tttcatggtg cggatttccg gacctttggt
    19561 atcaagcagg atagcggcgg ttttaccagt tttgctcatc acgttgcgca gattctgaat
    19621 gcgctgaccg tgttctgcat agtcaccatg agagaagttc agacgcataa cgttcatgcc
    19681 agcgtccagc attttagcta acatctcttc agattcggtt ttcggtccga tggtgcaaac
    19741 aattttggtc tttttcatga cagtcttagt ctttaagttg agaaggatgg gagaaacttg
    19801 ctttctgggc gcactgccgg aaagcgaatc cggtattacg aaagtggtgg tgcctcgctc
    19861 taaggatagg tgacatcgaa aaagcgtgca gaggaatgtg tgcttgtgtc tcagcccaac
    19921 gtgagtgtct gtttttgagt cgttgagttc tacagtccaa agtgctgaaa ccattcaaga
    19981 gtcaattggc gcgcattata cgctgaaatt aatccaaaag gaaaacaaaa acagcgtttc
    20041 aaaaaatata caacttgtta gacaataaat tgttatcaaa gatttaacgt tccagggaaa
    20101 aggttacgca aaaacttcgc tgtttgcgca acgtctggtg ccgaagttgt cagtgtgagg
    20161 ataagtgaaa gttcatccgg tgtataaata tatcatcgct caattgctac agttaaaata
    20221 atttatcgcg ttatttgtct gattttgttt gatttttgtt taattggtta gattgatata
    20281 tgctgata
//
LOCUS       DAEEUE010000074        20217 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.074, whole genome shotgun sequence.
ACCESSION   DAEEUE010000074 DAEEUE010000000
VERSION     DAEEUE010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20217
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.074"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            12..278
                     /gene="mliC"
                     /locus_tag="KLK84_002811"
                     /pseudo
     CDS             12..278
                     /gene="mliC"
                     /locus_tag="KLK84_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
     gene            337..993
                     /gene="pdxH"
                     /locus_tag="KLK84_002812"
     CDS             337..993
                     /gene="pdxH"
                     /locus_tag="KLK84_002812"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HBE2010068.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1122..2396
                     /gene="tyrS"
                     /locus_tag="KLK84_002813"
     CDS             1122..2396
                     /gene="tyrS"
                     /locus_tag="KLK84_002813"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBE2010069.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2455..3318
                     /gene="pdxY"
                     /locus_tag="KLK84_002814"
     CDS             2455..3318
                     /gene="pdxY"
                     /locus_tag="KLK84_002814"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HBE2010070.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3362..3967)
                     /gene="gstA"
                     /locus_tag="KLK84_002815"
     CDS             complement(3362..3967)
                     /gene="gstA"
                     /locus_tag="KLK84_002815"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HBE2010071.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4073..5575)
                     /gene="dtpA"
                     /locus_tag="KLK84_002816"
     CDS             complement(4073..5575)
                     /gene="dtpA"
                     /locus_tag="KLK84_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HBE2010072.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6186..6821)
                     /gene="nth"
                     /locus_tag="KLK84_002817"
     CDS             complement(6186..6821)
                     /gene="nth"
                     /locus_tag="KLK84_002817"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBE2010073.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6821..7516)
                     /gene="rsxE"
                     /locus_tag="KLK84_002818"
     CDS             complement(6821..7516)
                     /gene="rsxE"
                     /locus_tag="KLK84_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HBE2010074.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7520..8140)
                     /gene="rsxG"
                     /locus_tag="KLK84_002819"
     CDS             complement(7520..8140)
                     /gene="rsxG"
                     /locus_tag="KLK84_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HBE2010075.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8144..9202)
                     /gene="rsxD"
                     /locus_tag="KLK84_002820"
     CDS             complement(8144..9202)
                     /gene="rsxD"
                     /locus_tag="KLK84_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HBE2010076.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(9203..11425)
                     /gene="rsxC"
                     /locus_tag="KLK84_002821"
     CDS             complement(9203..11425)
                     /gene="rsxC"
                     /locus_tag="KLK84_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBE2010077.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVAAAIARVQAKKAAQQKVVNED
                     "
     gene            complement(11418..11996)
                     /gene="rsxB"
                     /locus_tag="KLK84_002822"
     CDS             complement(11418..11996)
                     /gene="rsxB"
                     /locus_tag="KLK84_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HBE2010078.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(11996..12577)
                     /gene="rsxA"
                     /locus_tag="KLK84_002823"
     CDS             complement(11996..12577)
                     /gene="rsxA"
                     /locus_tag="KLK84_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBE2010079.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(12654..13094)
                     /locus_tag="KLK84_002824"
     CDS             complement(12654..13094)
                     /locus_tag="KLK84_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HBE2010080.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(13180..13395)
                     /gene="ydgT"
                     /locus_tag="KLK84_002825"
     CDS             complement(13180..13395)
                     /gene="ydgT"
                     /locus_tag="KLK84_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HBE2010081.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(13668..13793)
                     /gene="blr"
                     /locus_tag="KLK84_002826"
     CDS             complement(13668..13793)
                     /gene="blr"
                     /locus_tag="KLK84_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HBE2010082.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            14036..15076
                     /locus_tag="KLK84_002827"
     CDS             14036..15076
                     /locus_tag="KLK84_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBE2010083.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKYVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(15112..16113)
                     /gene="add"
                     /locus_tag="KLK84_002828"
     CDS             complement(15112..16113)
                     /gene="add"
                     /locus_tag="KLK84_002828"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBE2010084.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(16217..17389)
                     /gene="malY"
                     /locus_tag="KLK84_002829"
     CDS             complement(16217..17389)
                     /gene="malY"
                     /locus_tag="KLK84_002829"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HBE2010085.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(17399..18991)
                     /gene="malX"
                     /locus_tag="KLK84_002830"
     CDS             complement(17399..18991)
                     /gene="malX"
                     /locus_tag="KLK84_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HBE2010086.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            19166..20194
                     /gene="malI"
                     /locus_tag="KLK84_002831"
     CDS             19166..20194
                     /gene="malI"
                     /locus_tag="KLK84_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HBE2010087.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
BASE COUNT         5140 a         5120 c         5199 g         4758 t
ORIGIN      
        1 aaatataggg gcaaatccag gttgagcgaa tgcaaaccga tacgctggaa taccagtgtg
       61 atgaaaaacc gttgacggtc aaactgaata atccgcgcca ggaagtcagt tttgtttacg
      121 ataatcaact actgcatctc aaacagggcc tttcagcctc tggcgcgcgt tactccgacg
      181 ggatctatgt tttctggtcg aaaggtgagg aagcgactgt ctataaacgc gaccgcatcg
      241 tcttgaataa ctgtcagtta caaaatccac agcgttgaga tttttccagg ggcggcgcac
      301 aatagcgtca cccactgaca atccgtaaag aaaaccatgt ctgataacga cgaattgcag
      361 caaatcgcgc atctgcgccg tgaatacacc aaaggcgggt tacgccgccg cgatcttccc
      421 gccgatccat taaccctttt tgaacgttgg ctctctcagg cttgtgaagc caaactggcg
      481 gaccctaccg cgatggtggt cgctaccgtg gatgaacatg gtcagcctta tcagcgcatc
      541 gttttactca aacattacga cgaaaaaggc atggtgtttt acaccaacct cggtagccgt
      601 aaagcacatc aaattgaaaa taatccgcgc gttagcctgc tgttcccgtg gcataccctt
      661 gaacgccagg tgatggtgat cggtaaagca gaacgacttt cgactctcga agtgatgaaa
      721 tattttcata gccgcccgcg tgatagccag attggtgcat gggtttcgaa gcagtccagt
      781 cgcatttctg cccgcggtat ccttgaaagt aaattcctgg agctgaagca gaagtttcaa
      841 cagggcgaag tgccattgcc gagcttttgg ggcggttttc gcgtcagcct tgaacagatt
      901 gagttctggc agggcggtga gcatcgcctg catgaccgct ttttgtacca gcgtgaaaat
      961 gatgcgtgga agattgatcg tcttgcaccc tgaaaagatg caaaaatctt gctttaatcg
     1021 ctggtactcc tgattctggc actttattct atgtctcttt cgcatctggc gaaaagtcgt
     1081 gtaccggcaa aggtgcagtc gttatataca tggagatttt gatggcaagc agtaacttga
     1141 ttaaacaatt gcaagagcgg gggctggtag cccaggtgac ggacgaggaa gcgttagcag
     1201 agcgactggc gcaaggcccg atcgcgctct attgcggctt cgatcctacc gctgacagct
     1261 tgcatttggg gcatcttgtt ccattgttat gcctgaaacg cttccagcag gcgggccaca
     1321 agccggttgc gctggtaggc ggcgcgacgg gtctgattgg cgacccgagc ttcaaagctg
     1381 ccgagcgtaa gctgaacacc gaagaaactg ttcaggagtg ggtggacaaa atccgtaagc
     1441 aggttgcccc gttcctcgat ttcgactgtg gagaaaactc tgctatcgcg gcgaacaact
     1501 atgactggtt cggcaatatg aatgtgctga ccttcctgcg cgatattggc aaacacttct
     1561 ccgttaacca gatgatcaac aaagaagcgg ttaagcagcg tctcaaccgt gaagatcagg
     1621 ggatttcgtt cactgagttt tcctacaacc tgttgcaggg ttatgacttc gcctgtctga
     1681 acaaacagta cgatgtggtg ctgcaaattg gtggttctga ccagtggggt aacatcactt
     1741 ctggtatcga cctgacccgt cgtctgcatc agaatcaggt gtttggcctg accgttccgc
     1801 tgatcaccaa agcagacggc accaaatttg gtaaaactga aggcggcgca gtctggttgg
     1861 atccgaagaa aaccagcccg tacaaattct accagttctg gatcaacact gcggatgccg
     1921 acgtttaccg cttcctgaag ttcttcacct ttatgagcat tgaagagatc aacgccctag
     1981 aagaagaaga taaaaacagc ggtaaagcac cgcgcgccca gtatgtactg gcggagcagg
     2041 tgactcgtct ggttcacggt gaagaaggtt tacaggcggc aaaacgtatt accgaatgcc
     2101 tgttcagcgg ttctttgagt gcgctgagtg aagcggactt cgaacagctg gcgcaggacg
     2161 gcgtaccgat ggttgagatg gaaaagggcg cagacctgat gcaggcgctg gtcgattctg
     2221 aactgcaacc ttcccgtggt caggcgcgta aaactatcgc ctccaatgcc atcaccatta
     2281 acggtgaaaa acagtccgat cctgaatact tctttaaaga agaagatcgt ctgtttggtc
     2341 gttttacctt actgcgtcgc ggcaaaaaga attactgtct gatttgctgg aaataatgca
     2401 ttaagtggaa agggggagtg agaaatcact ccccctggtt tttatacagg gaacatgatg
     2461 aaaaatattc tcgctatcca gtctcacgtt gtttatggtc atgcgggtaa cagtgcggca
     2521 gagtttccga tgcgccgcct gggcgcgaac gtctggccgc tgaacaccgt tcaattttct
     2581 aatcacaccc aatacggcaa atggactggc tgcgtgatgc cgcccagcca tttaaccgaa
     2641 attgtgcaag gcattgccgc cattgataaa ttacacacct gtgatgccgt attaagtggc
     2701 tatctgggat cggcggagca gggtgaacat atcctcggta tcgtccgtca ggtgaaagca
     2761 gcgaatccgc aggcgaaata tttttgcgat ccggtaatgg gtcatccgga aaaaggctgt
     2821 atcgttgcac cgggtgtcgc agagtttcat gtgcggcacg gtttgcctgc cagcgatatc
     2881 attgcgccga atctggttga gctggaaata ctctgtgagc atgcggtaaa taacgtcgaa
     2941 gaagcggttc tggcagcgcg cgaactcatt gagcaagggc cacaaattgt gttggttaaa
     3001 cacctggcgc gagctggcta cagccgtgac cgttttgaaa tgctgctggt caccgccgat
     3061 gaagtctggc atatcagccg tccactggtg gattttggta tgcgccagcc ggtaggtgtt
     3121 ggtgatgtga cgagcggttt actgctggtg aaactgcttc agggggcaac gctgcaggag
     3181 gccctggaac atgtgaccgc tgcagtctac gaaatcatgg tgaccaccaa agcaatgcag
     3241 gaatatgagc tgcaagtggt ggctgctcag gatcgtattg ccaaaccaga acattacttc
     3301 agcgcaacaa agctctgaaa tatctcattt cggcccacaa cgacctgtgg gccgatggct
     3361 attactttaa gccttccgct gacagcgcgt cttgtacttc cggacgttca gccatacgtt
     3421 gcataaatgc cgcaatgtgc tctaaccctt ccagattcag tttcaccgcg tatgcccagc
     3481 gcagaaccgt aaacagatag gcatcagcaa ttgtaaatct ttgcccgcag atccagtgct
     3541 catccttcag tgcctcgttc acatattgca gcttcttatc cagctgcgcg cgaactgtcg
     3601 gtttgtactc ttccggtgta tctgggcgaa acagaggtgt gaaaccttta tgcagctcgg
     3661 tggcgatgta attcagccat tcgatggttt tatagcggga aatactgttt accggtgcca
     3721 gcaactggcg gtcggggacg ctgtcggcaa gatactgcat aatcgcaacg ccttccgtca
     3781 gcaaagtacc atcgtccagc agcaatgcag gcacctgccc cttagggtta acggaaaagt
     3841 aatcgtcacc gttttcgaga cgttttttca ttaaatccac actgacgagc gtaaaatcct
     3901 ttccgctctc acgcagggtg atatgggaag cgagagagca ggcgcccggt ttgtagaaca
     3961 atttcatcgg taactccttt tgtctgtggt ttaaagtatg gtagtgcgtt gctgaacaaa
     4021 aaaaaagccg ctaacgaaaa gttagcggct tgtaaaagag tttccctgaa ttttacgcta
     4081 cagtagcttt cgccgctttg tctgcagcgt catcctgcgt catgcggtgc agtttaggcg
     4141 cggtcagcag catcagtact gcaataacgg cagtagcgac accaatctgc aagaatactc
     4201 gaccatagac ttccagtgac atcagcgggt cggtaacgtt atccggcaca gccatcatac
     4261 ccgcaacata accaccaatc aggtttgcac cggcagtggt caggaaccag ctacccataa
     4321 tgaagcccat cagacgctgc ggaacgagtt gtgcaaccat tgccagaccc agaccagaga
     4381 tcatcagttc cccgatgctc tgcaggccat agcttgcgac cagccagctg acagacacga
     4441 tacctgcgtc agacgcgaat ttcgcaccca gcggcagaat caggaacgca ccagaacaca
     4501 tcaccatgcc gattgcaaac ttggttggca tcggcagggt atcacccatc ttgttataga
     4561 tagcggccag aatcggacta ccgatgatga tccagaacgg gttcagtgcc tgatactgct
     4621 caggttctac ggccagaccc agaatggagt gctcaacgtt acgaatcgca aagaagttca
     4681 gtgacgttgg catctggctg tacagcacga agaagatgat tgcttcgagc atcaggatga
     4741 aggcaacgat cattttacga cgcgcagcac ctttcatcgc gaaagcttct ttaccgaaga
     4801 taaccacaat accgaaggca acaacgccca gcgccatacg cgcaacttcc tgattgtgca
     4861 gtagccaggt ggcaacagcg atcagcgcca caacaccaat aatggtcagc aacaggttac
     4921 ggtagttgac tggttcaaag tctggttttg aaccgtactg tttaacccag cgttggcaga
     4981 aggcgaagtt aacgatagtg atcagcaggc ctacaacgct caacgcaaac gcaacactcc
     5041 agccgtattt cgcggccagc cacggggtgg ctatcataga gaagaaagag ccgatgttga
     5101 cggacatgta gtacatggtg aatgcaccgt ccagacgtgg gtcgtttttc tcatagcatg
     5161 tagaaagcag agaagacggg ttagctttaa acaggccgtt accgaccgca atagccgcca
     5221 tacccatata aacaataccg gcgtcgtgac cagaccaggc aaccagagca taaccaatcg
     5281 ccagcacaat agcgccgagc ataattacgc gtttagtacc cagtaccttg tcacctaacc
     5341 agccgccgat agcgaccaga ccataaacca gggcactaaa ggaagagaaa agggtgattg
     5401 aatccgcttc agacataccc agttgtttaa ccaggtaaac agccataatt ccttgtaggc
     5461 cgtaataacc aaaacgttcc cataactcaa tcgagaagat gagatagaac gccttcggtt
     5521 gtttgaaagc gttcaaactg acgctttcag ttggtttttg gtttgcagtg gacactttta
     5581 cctctttttt tacatcccat attaacgggg gtgctttctc gcgttatgtc taaaaaacat
     5641 acgcgcaatt gttatagtgg gaggggaaac ggcaggtaat gttcactatc ttgcactttc
     5701 agacaagtcc tttgtaataa tctgttacat ataactgagg cggggaattc aaccattccg
     5761 ggaaatgtta tccagcgtta aatttttcaa aaaacgtaac ggcagatttt ttcactgcat
     5821 tgagaggggg atgatggctt ggcggcgata tgttctgcta tttagcgttt ttcctagtga
     5881 gatagtccag tttctgaaaa atagccagtg taatgttttg caggtcaata tttgttgcgt
     5941 gtgtaaattt aaatcatttt aacctgattc ttgcaatgtt tttacattgc cacagcatcg
     6001 tgatcttgat cacgaatgaa tagaaaattt cgctattaaa aaaacaatta acctgcattt
     6061 ttggttatcc gcaaacggat tacctgatga taacgctaag caatggcatc attgacgtta
     6121 aaaagatgtt ttaaaagagg ataaagaaag gttatcaatg gggtaatcgg tgttacccct
     6181 tttcttcaga tgtcaacttt ctctttgtat tcacaaagat cttcaataat acaagagcca
     6241 cagcggggct tgcgggcaat gcaggtataa cgcccgtgca ggatcaacca atggtggcag
     6301 tcgactttaa actctgccgg aaccactttc agtagctttt cttctacctg ttcgacgttt
     6361 ttccccggcg caaattgagt acgattacaa acgcggaaaa tgtgcgtgtc gacagcaata
     6421 gtcggccagc cgaatgcagt gtttaatacg acgttggctg ttttacgacc tacgccgggc
     6481 agggcttcaa gcgcagcacg atcttccgga acctcgccat tatgttgttc cagtaatata
     6541 cggcaggttt tgatgatatt ttctgctttg ctgttataaa ggccaatcgt tttgatatag
     6601 gttttcaccc cttcaacgcc tagttcaagc atcgctgcag gcgtattcgc caccgggtag
     6661 agtttcgccg tcgccttatt aacactgata tcggtcgcct gagcggaaag cagtacggca
     6721 atcagcaatt caaaaggcga actgaaatta agctcggtgg tgggatgagg attgttctca
     6781 cgcaggcgag tgaggatctc cagacgtttt gctttgttca tcagacattc cctgtttcac
     6841 cgtttggcaa tgcacgttcg gcagctgctt cagcacggcg ctttttcatt ctttcatcaa
     6901 tcaggtattt tcctgccagc atcagtccca agccaataaa tgcacctggt ggcagcatcg
     6961 ccagcaggaa aggggagtcg gtgtggaaaa tctccacgcg taatactttt gcccagctac
     7021 ctaacagcgc atctgcaccg tcaaacaacg tgccattgcc gataatttcg cgtagtgaac
     7081 ccagcacgaa catggcgcag gttgcgcccg taccaattga aaagccgtcc agtgccgaaa
     7141 gcgccggacc ttttttggcg gcgaaggctt cagcgcggcc cacaacgata cagttagtga
     7201 caatcagcgg aataaaaatc cctaatgatt gatacaggcc aaaggcgtag gcgttgatca
     7261 gcatctgtac agcgctgacc accgaggcga tgatcatcac gtaaatggga atgcggatct
     7321 cggctggcgt ccagtgacgc agcgtcgaaa tggtcaggtt ggtcagcgtc agtaccagcg
     7381 tagtcgcaag tcctaaaccc agagcgttag tggcagtgga tgtgaccgcc aatagcggac
     7441 aaaggccgag cagttggacc agtgcagagt tgtttttcca caacccctga acaataacgt
     7501 ctttaatttc gctcacgctt tattctccac aggcaggaag ttgagaaagt tgtgccggta
     7561 acgtctgagc gtacaatccg gcgcgtttta ccgcattaac caccgcgcgg ggagtaatcg
     7621 tcgcgccggt gaactggtcg aaatcaccac catctttctt caccgcccag tgcgcatcat
     7681 ccgcaccact gatttttttt cccgcaaaat gggtgagcca gtcagaaagg cgcaattcga
     7741 ttttatcgcc aagccctggc gtttcgtggt gctctgtcac gcgcgtgcca agtaccgtgc
     7801 cattaaaatc ggctcccacc agcagctgaa tcgcaccgga atagccatct ggcgcggttg
     7861 cttccagaac ggcggctacc ggtttgtcat cctgtttggc gatgtaaacc cgatgctcac
     7921 ctttacctaa ctctggcgca gttaccagat agcaactctg tgcgagcgca ttgttatagc
     7981 gttcggctgg cagcacctga tcaaataacg tcttttgttg cagactggcc tgttcagcaa
     8041 ttgtcgtttt ggtcatctgg ttgatggccg cagttaaccc tgtagaaccc gctgcaaata
     8101 gtgccagtgt aatgccgtgt tttcggatag ttttcagcat ggtttaccct ttgcgatggc
     8161 cgtagacgcg cggacgcgtg tagtaatcga tcagaggaac cgtgatgttc gccagcagga
     8221 cggcaaaagc cacgccgtca ggatagccgc cgaaactgcg gatcaaccag actaataagc
     8281 ccgccagcgc gccgaacatc agccgaccac gattggtcgt agaagcagta actgggtcag
     8341 tcaaaataaa gaatgcgccg agcatagtcg caccagacag cagatgaatt tgcggtgctg
     8401 ccagtgtttc tggtgagaac aaccagccca acgttgcgca taacgccagc gttactaaga
     8461 agctgagggg aatatgccag cgaatcgctt tctgccatag caaccacacg ccaccagcca
     8521 gccaggcgag atttacccat tgccaaccag cgcccgccag aataccgctg tagatcggat
     8581 attgcataat ctgttcaacc gaatgacctg cgcggacaga ggttttgaat gtatccagcg
     8641 gtgtcgcctg actaatgcca tcaataccta agcgtagcgt attcatatca ccaccactgg
     8701 cggtatgacc gctgaaaata acctgtatgg cgtcgataaa accagggatg ttgaccgcaa
     8761 tttcatgtgg cggtaaccag ctggtcatct gcaccgggaa ggagatcagt aagaccacat
     8821 aaccaatcat tgccggatta aacgggtttt gccccagacc gccatacaac tgtttagcga
     8881 tgatcaccgc aaacaccgta cccagtacga ccatccacca tggcgctagg gggggaatac
     8941 ttaccgccag caataagcct gtcagcaatg ctgagttatc tttcaacgtt gcggctaccg
     9001 actgcttgcg tagtttgagt acgagagctt cggctaacag agcactgacc gacgccaaca
     9061 ggatctgaac gagagtaccc caaccaaaaa accacagttg cgctgcgatt cctggcacgg
     9121 ctgcgagcaa caccaacagc ataatgcgcg atgtctggcg ctggttatgg gtataagggg
     9181 agctagctat tctgaatacc atttagtcct cgtttacaac cttttgctgg gcggcttttt
     9241 tggcctgaac gcgggcaata gccgcggcaa ctgcggcttt gcgcggatcg acctgttctt
     9301 ctggttccgc attagcctgt tgctgttcca gcttgcgtgc tttggcgcgg gcaatagcgg
     9361 cttcgacggc agctttgcgc ggatcaacct gttcttctgg ctccgcattg gcctgttgct
     9421 gttccagctt gcgtgctttg gcgcgggcaa tagccgcttc gacggcagct ttgcgcggat
     9481 caacctgttc ttctggctcc gcattggcct gttgctgttc cagcttgcgt gctttggcgc
     9541 gggcaatagc ggcttcgacg gcggctttgc gcggatcgac ctgttcttct ggttccgcat
     9601 tagcctgttg ctgttccagc ttgcgcgctt tggcacgggc aatagcggct tcgacggcgg
     9661 ctttgcgcgg atcgacctgt tcttctggtt ccgcattagc ctgttgctgt tccagcttgc
     9721 gcgctttgga gcgggcgata gctgcttcaa cggcagtttt acgtggttca gcaacggttg
     9781 ctgcgtcgtt agtttgctcc agttctgcct gtttcgctct ggcttgcgct ttacgggctt
     9841 cccgttctgc aataattgca ctgttatccg ggcgttcgcc cgctttaatc acaataggct
     9901 gtgtagcctg ggcctgtttc tctttcaccc gcgccagagc ggcagcaatc gcatctttat
     9961 ctttggctgc aggttgaacg gctgcgctct tatgtcgttc aaggcgagtc gctttttcgc
    10021 gctccagacg agcctggcgc gcttcgaaac gcgctttggc ttctgcggcg cgcttttctt
    10081 cctgacgaat agccgcaatt tcagcttttt cctgacggaa atattgcacc aagggaatat
    10141 tgctcgggca aacccacgcg caagccccac attcaatgca gtcggcaatg ttatgcgtgg
    10201 tagctttatc gtgttgctga cctttgctga accagtacaa ctgttgcggc aaaagatcca
    10261 cagggcaggc gtcagcacag gcgctacacc ggatgcagct ttgttcttcc tgtggttcgc
    10321 caagttcatt ggcagaggga gccaacagac agttggtaat ctttacgacc gggacatcca
    10381 gccatggcaa ggtaaagccc attagcgggc cacccataat caccatttga tcggcagagg
    10441 ggcagaatcc ggcatcattc aataaatgac gcactggcgt ccccagccgt gcccagacgt
    10501 tgcccgggcg agcgattgct tcgccagtca gggttacaac acgctcggta atcggctcgc
    10561 catcaataac ggcacgtttc actgcataag cagtgccgac gttttgcatt aatacgccga
    10621 tatcggatga acgcccgcca tgtggaacct gcttcccggt cagaatgtag gttaattgtt
    10681 tagcaccgcc agaaggatat ttggttggaa tcacccgcag agaaatatcg ttagagtccg
    10741 ccagcaccgc gcgcagcatg gaaatcgcct gcggtttgtt atcttcaatg ccgataagaa
    10801 tttcgcgtgg ctgcagaata tgcgcaagaa tgcgaatacc ctctacgacc tgagccgcgc
    10861 aatcctgcat caaacggtca tcggcggtaa tgtacggctc gcactcagca gcgttgataa
    10921 tcaacgtttc aatcttatct ccaccaccct gcaatttaac gcctgtcggg aatccggcac
    10981 cgcccagccc ggcaacacca aactgatgta tgcgctcgat taactcttcg cgactgcgag
    11041 agcgataatc gacccagccg tcgcgcggga tccagcagtc ttcaccatcg gcatcaataa
    11101 tcacgcttaa ttcagctaaa gctgaaggat gagccgtaga gtggggcgca atagccgtaa
    11161 cggtacccga ggtgggcgcg tgaacaggca gcattttgcc gcgaccacgg gtaagcggct
    11221 ggccgcgcaa tactttatcg ccgacgctaa cgcacaactc accttcagcg ccaatatgct
    11281 gtttcagtgg aataacaaaa tgctgcgcca gtagtacctg gcgcaggggg gtaccgttgg
    11341 tctgggtttt catctccggt ggatggatgc cgccgttgaa atcccagatt ttattttttc
    11401 tgaatgcaga gaataactta agcatggtgt tccacgggaa tgatacgcac gggaatggtg
    11461 ttcagatccc atttccagga gtcaggtgtt tctgcgaccg gttgcaacga gatgcagtgc
    11521 gtcgggcacg gatcaacaca taaattgcag cccgtacaga gatcactcat taccgtatgc
    11581 atgacacggg tagcgccaac gatggcgtct accggacaag cctgaataca tttagtgcag
    11641 ccaatacagt tattttcatc aataaccgcc accatccgcg caggcgttag ctcttgcgct
    11701 tcgccatcca gcggctgcgg ctcgacatta agcaactcgg caatttttag catcacagct
    11761 tcgccacctg gggcgcaacg gttgattttt tcaccgttac agctgatggc ttccgcgtag
    11821 gggcgacagc cgggataacc gcactgacca cactggctct gcggtaagat ttcgtcaatt
    11881 ttctcaacga ccggatcgtc ttccaccgca aaacggcggg aggcataacc cagaatggcg
    11941 ccaaacgcca ggcccagcag gctcacggcg gcaacggcaa tccagatagc attcattaca
    12001 acttcaccaa accactaaag cccataaagg ccagagacat aagacctgcg gtaattaacg
    12061 caatggcatt accgcgaaaa ggtgccggga catcagccac agcaaggcgt tcgcggatgg
    12121 cggcgaagag caccatcacc agcgagaaac cgacagcggc ggaaaaaccg tacagcgccg
    12181 actgcaagaa attgtgcccg agattgatat tcagcaacgc cacgccgagc actgcacagt
    12241 tggtggtgat aagcggcaaa aaaatcccca gcagacggta aagcaccggg ctggttttgc
    12301 gcaccaccat ctcggtgaac tgcacgacca cagcaatcac cagaataaat gccagggtgc
    12361 gcaggtaaat cagattaagt gggatcaaaa tccacgtatc gataagccag gcgcaaatag
    12421 acgtcagcgt catcacaaac gttgttgcca gccccatgcc catcgcggtt tccagttttt
    12481 tggaaacccc cataaacgga cagagaccga gaaacttgac cagtacaaag ttattgacca
    12541 gtacagttcc gacaaacagt aacaggtagt cagtcatttt tcggcctgaa ataaaaaagc
    12601 cgcctattat ccgttaatcc agagcaggcg acaacaggtt aactgtaagg ttattacgga
    12661 ttcacaaacg tcgcttttac ccgcgacgag cgtttgaaat aggggacgat caatgcggtt
    12721 gccaacaaag taaacagcaa ctggcgaacg gctatgccaa cttccacggg tgaaaaagcg
    12781 aatgctttta ctgccagcaa tacggaaata agcagccaga taatatagtg tttaggaaca
    12841 caacgccgac gtttaaagaa agcgatggtc aaccacagtg tgtaatacca catggcgata
    12901 gcggtaataa aagaaacgcc ccataagatt tgtgtcgtca gtgcttgtcc gccgagcgtt
    12961 tgaaatgttt gcggagaaga taacgcagcg gtatacagca acaacgccag cgtcgtactc
    13021 aacagagcga ctaaaagcca ggccaacggg ccaagcaacc agcctccaat acgttgtggc
    13081 gttgtggtgg tcataggttc tccagaatag cgacgcaaac aggcaaatct aattttaggg
    13141 gcgttattat aaggctttta taaccgatcg ctactttctt tattggacat agtgccagac
    13201 ggacttcggt acctggccga ggtcaaacaa acgcccgcca gaaaccagct ctgcgcgacg
    13261 gtgatcggca gcacgataca tattgatcag ttcctgatcg tccgtcaggg tgtaattaag
    13321 atggtcgtag agtttttcca gactttcgag tgaactgatt ttgcgaaatt ttaataagta
    13381 gtcctgaaca gtcataaaag gtccataaaa taattaatac caaaggggta ggtattatta
    13441 gtgatgataa aatttcacca aaagtgaacg cttgtgttat tacaataata agaatgcgct
    13501 gatggcaaat attcagggat gaaaaactgc tctgaaaaga gtaacgcgct ttactatccc
    13561 tgacaatcac caacaacatc gaacaagata ataaattcct ggtttaatat ccgacaagtg
    13621 aaaacatgca cccggacggg cagcatgtcg ctccacaagt gcagagctta cttgtgttgt
    13681 accgaagcac tctgttcagg tggctgatag ttgtcaatgt gactcgccac gccaagaaga
    13741 atgactgaaa cgacaagaac gatccaacct gttaattcaa taagacgatt cattacagcc
    13801 cacatctctc ttgattgatc cattaacttc agggggtaaa tgttacttag caataatagc
    13861 tccagcaaga tttttactga ggtttttgcg atatttagct tttgtcgttg gaaaattcgc
    13921 tatttttttg acttaagtta aacaacatcc cttattgctg gcaggttatt aaactgttga
    13981 gcgtgggtaa aggatagtgt caaatagcca tcatacttca atgagaggca atgacatgag
    14041 cgacaacatc cgtgttgggt tgattgggta tggttatgcg agcaaaacct tccatgcgcc
    14101 cctgattgcg ggcacgcccg ggctggaact ggcggtaatc tccagcagtg atgaaacaaa
    14161 agtaaaagcc gactggccaa cggttacggt tgtctctgag ccgaagcatc tgtttaacga
    14221 tcccaacata gacctgattg tcattcctac acccaacgat acccatttcc cgttagccaa
    14281 agcggcgctt gaggcgggta aatatgtggt cgttgataaa ccctttaccg tgacactgtc
    14341 acaagcgcga gagctggatg cgctggcaaa aagcctgggg cgtgtgctgt ctgtattcca
    14401 taaccgtcgt tgggatagcg atttcttgac gctaaaaggt ttgctcgcgg aaggcgtgct
    14461 gggtgaagtt gcttactttg agtctcattt tgaccgcttc cgtccgcagg tgcgcgatcg
    14521 ttggcgtgaa cagggcggcc caggcagcgg tatctggtac gatttagcac cgcatcttct
    14581 tgatcaggcc attacgctat ttggtttacc ggtcagcatg acggtagatt tggcacagtt
    14641 acggcccgga gcgcagtcga ccgattattt ccacgccatc ttgtcctatc cacagcggcg
    14701 agtcatttta cacggtacca tgctggcagc cgctgagtca gcacggtata tcgtgcatgg
    14761 atcccgaggc agttatgtga aatatggcct cgatccacag gaagaacgtc tgaaaaatgg
    14821 cgagcgtctg ccgcaggaag actggggcta cgatatgcgt gatggcgtac ttacccgcgt
    14881 ggaaggtgag gaacgtgtcg aagaaacgct gttgacggtg cctgggaatt atccggctta
    14941 ctatgcggcg attcgtgatg cgttaaatgg cgatggtgaa aatccggttc cggcaagcca
    15001 ggcaatccag gtaatggagt tgattgagct gggcatcgaa tccgccaaac atcgcgcgac
    15061 gttgtgcctt gcgtgaaaaa tggatggtgc aatttgcacc atccattttt gttacttcgc
    15121 ggcgactttt tctcgcagtg cgcgtttttc ctctgcgctg aggaaagcca tttccagacc
    15181 attaatctgt gcctggcgga tttgctcacg ggataaccca gcagctggcg cggcaacggt
    15241 atattcgtga atgatatcca ctccctgtac gccgggatcg tcagtgttaa tgctggcacg
    15301 aatgccatgc tcaaggaacg ttttcagcgg atgtgcagcc agctctgcta cggtgctggt
    15361 ctgaatattg gaggtcagac aggattcaat accaatttgt tgctcggcga gaaaatccat
    15421 cagcgcccga tcttcaatgg cttttacgcc atgtccaata cgctccgcac ccagttcacg
    15481 aatcgcctgc cagatgcttt ccggcccggc agcttcgcct gcatggacgg taatatgcca
    15541 gcccgcatca cgcgcgcggt tgaagtgaga aaggaacaga cttcccggga atccaagttc
    15601 atcaccagct aaatcaagtg cggtaatctg gtcacggtgg gctaaaaagg cctccagctc
    15661 ttgctgacag gcggcttcgc cgaaggttcg gctcataatg ccgataagct tcgcctgcac
    15721 accaaaggtg cggcaacctt cacgtacgcc atcgatcacc gcttcgacaa cacccgctac
    15781 aggcagctta tgtgccattg ccatgtagcc tggtgaaaaa cgcagctcga catagcgcag
    15841 gccgttacgg gctgcatctt caatgttttc aaatgccacg cggcggcagg catcaagaga
    15901 ggcgagaact ttaacgcccc agtcaagttt ggtcagaaag ctcaccagat cgggttcgtt
    15961 ggcaatgacc tgaacgtggg gaatcagtgt ttcaagggat tgtgcaggaa gcgagatatt
    16021 atactggcgg ccaagttcaa gaatggtctg gggacgaatg ttgccatcaa ggtggcgatg
    16081 gatatcagtt aatggcaggg tggtatcaat catggtcgca ctctttttct ggatataaaa
    16141 gtgcgcacca ttataaaaac aaagcagggt aaaaatccac gattgcgcaa cgatttttta
    16201 cgttgcgcaa ttggggttag cgaacagcgc ggatggcgtt tattaatcca gccacacctt
    16261 tttccagttt cgaacgtggg cagccggcat tgagacggac aaaaccacga ccttcttcac
    16321 cataggtata ccccggcatg atcgcgactt tttcttgctc gataagcgct ttttgcaacg
    16381 cgttgtcgtc aatattcaac ggacgtagat caagccaggc cagataagtg gattgcggga
    16441 tctgccagtt gagttcagga aacgcggcgt tcattttatc tgagatatac gtcaggttat
    16501 ctttcagata gacgcgtaag gcatccagcc acggtgcacc ttgctgatag gcggcgatat
    16561 gggcagttaa cgccagtacc gaaggggaag aaagcccatc acgtcctttc agtgccgata
    16621 aataggcatc acggctgctg ctattttcta taatcccgta agcaccggtc agcgcgggaa
    16681 tattgaaact tttcgatccc gatgtcagca acgcccagtc tccccgcgcc acattactcc
    16741 agggaatatg cggctgctcg ccccagacca tatccatatg gatttcatcg gaaataaccc
    16801 gcacatcatg acgctcgcac aggtcagcca tgatctccag ctcatcgcac gtccacactt
    16861 tcccggtagg attctgtggg ttacacagga gcataatttt acattctggt ttcgccaaaa
    16921 cggcttccag cttgcccata tcgcaaaacc agccgtcagc ctgcttctct aaagcaacgg
    16981 gcattactgt gcgctggtta ccttcaatgg ccttgtaaaa tgcgtcatag gcgggcgtgt
    17041 ggatcaccac gccttcacct gtttcagacc actgacgaat cagttctgaa accatataga
    17101 tgacagaagg gccatacacc accgtctgcg tatcaattgc ggtgtaatgc tgggtggaaa
    17161 accagtgggc aatagccgcg agaaactcat cgtttttcca gcggctgtag ccaaatacgc
    17221 cgtgcatcag gcgctgattc agcgcctcga taatgcaggg ggcagtggca aaatccatgt
    17281 ctgaaatcgt gaacggtaac aggtcagcag tgccgaaacg gtcagcgaca taatcccact
    17341 gtgtacacca tgtgccatga cgatccacga cctttgaaaa atcgaacata tcttatcctt
    17401 atgcctggac agtatgcatc agaccggcca tttcatcttt tactgactga acttgtggcc
    17461 cgataacaac ctgcaggtta tgttgattaa gttgtactac gccaattgcc cgattgtcct
    17521 tcagtgcctg cacattaaca agcgacatat cttccacaga caaacgcaga cgggtaatgc
    17581 agttatcgag gctgacaata ttgtcggcac cgcctaatgc ttcgaggatt gcaggaacgt
    17641 tgtaacctga tttacccggc gcaccggcaa cggctttttc gattgagctg gcaacttcgc
    17701 tatcgcgccc cggggttttc agattgaagc gggtgatagc gaaacggaag atgacgtagt
    17761 aaacgacaaa ccagattgcc gccaccactg gcaccatgta ccacttggtt gacagaccat
    17821 gcaaaatacc gaacaccacg aagtcgatga tattgccgtc ggtattaccg atggtgacgc
    17881 cgagcacaga catgacggtg aagccgaggc cggttaacag cgcgtggatg acatacagaa
    17941 ctggcgctac gaacaggaac aggaattcca gcggttcggt agtgccgcca acgacgcagg
    18001 cgatcaggcc agaaatcagc agacctttaa ttttatggcg attttccggg cgcgcgcagt
    18061 gatacatagc taacgctgca cctggtagac cgccgagaaa cgcaggcatt ttaccttgcg
    18121 aaaggaaacg cgtgacgctt tcagaaaaac cgtgagtggt cgggcaactc aattgcgcct
    18181 ggaagatggt cagtgcgccg ctgacggttt gaccgcagac ctcctgcgtg ccgcctgcgt
    18241 cggtaaagcg aattaatgcc accagaatgt gatgcagacc aaacggcaac agcagacgtt
    18301 caccggtacc aaacagcatc ggtccgaaat cacccgcgct gtttatcata tggcccaagc
    18361 cgctaatacc catggcgaaa atcggccaga ctaatggaat caccaggccg acaaggccca
    18421 tcaccagcga ggagataatt ggtacgaagc gcgtaccgcc gaagaatgcc agcgcatccg
    18481 gcaggcggat attatggaaa cgctcatgca gcatccagac gataataccg gcgatcaccg
    18541 caccgaggat cccggtatcg atcgactgga tcccaagaat gctctggatg ttattggctt
    18601 tcagaacggc ggcatctgtg gttggcagaa tgcctttatt ggtcaaccag aagtttaccg
    18661 cgaggttcat taccgcataa ccgacgaagc cagcgaatgc cgctacgcct ttattttcgc
    18721 gtgccaggcc cagcgggatg gcgatacaga acatgacagg caggaaacta aaagcaaacg
    18781 agccaatctt actcatccag gtaaagatag cctgcaacac ggggttgccc aggaccggga
    18841 tcagggttat gacatcatgg ctgctaagag aactaccaat gccgagcata atgccgcaga
    18901 acgacaataa tgccacgggt aacatgaagg ttttgcctaa ctgctggaag aactcccaca
    18961 gcgtgacttt cggtgctgtt ttcgccgtca taaaacgact cctcgtagag aacaatctgg
    19021 attcagtaaa ttgcgcgaga agataaaacg ttttatcaaa ttttagtgag tcataaatca
    19081 cattacgcaa cgataatagc gggtataaga taaataaaag gtaaaacgtt ttatctgtca
    19141 cataatcagg gagtaggtca tctgcatggc taccgccaaa aaaataacca ttcatgatgt
    19201 tgcgctggct gcgggcgtgt cggtaagtac cgtttcgctg gtgcttaatg gcaaagggcg
    19261 aatctctacc gccacaggag aacgcgttaa cgccgccatt gaagagctgg gatttgtgcg
    19321 caatcgacag gcgtcggcgc tgcgcggcgg gcaaagcggc gtcattggtt tgatcgtccg
    19381 tgatttatct gcgccgtttt acgccgaatt gacggccgga ttgacggaag ctctggaagc
    19441 acagggacgg atggtttttt tgcttcacgg cggtaaagac ggcgagcagc tggcacagcg
    19501 gttttcactg ttactgaatc agggtgtcga tggtgtggta attgccgggg ctgcaggaag
    19561 cagcgatgac ctgcggcgga tggcagaaga aaaagctatc ccggtgattt tcgcttcccg
    19621 tgccagttat cttgatgatg ttgatacggt tcgcccggac aacatgcagg ctgcacagtt
    19681 gttgacggag catctcattc gcaatgggca tcagcggatc gcctggctgg gagggcaaag
    19741 ttcctcatta acccgtgcag aacgggtggg gggctattgt gcaactctac taaaatttgg
    19801 cctgccgttt cacagcgatt gggtgttgga gtgcacttcc agccagaagc aagccgcgga
    19861 agctatcacg gcgcttttac gtcataaccc gaccatcagt gccgtggttt gctataacga
    19921 aactattgcg atgggggcat ggtttggttt gctgaaagca gggcggcaaa gcggggaaag
    19981 cggagtcgat cgttactttg agcaacaggt ttcgctggcg gcatttaccg atgcgacacc
    20041 aaccacactt gatgatatac ctgttacctg ggccagcacg ccagcgcggg aacttggtat
    20101 cacacttgcg gatcgcatga tgcaaaaaat cacccatgaa gagacgcatt cacgcaatct
    20161 tattattccc gcccggctca ttgcggcgaa ataatcctct ctttatctgc tatactt
//
LOCUS       DAEEUE010000075        20084 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.075, whole genome shotgun sequence.
ACCESSION   DAEEUE010000075 DAEEUE010000000
VERSION     DAEEUE010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20084
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.075"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            376..579
                     /locus_tag="KLK84_002832"
                     /pseudo
     CDS             376..579
                     /locus_tag="KLK84_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            598..5262
                     /locus_tag="KLK84_002833"
                     /pseudo
     CDS             598..5262
                     /locus_tag="KLK84_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390554.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            5265..5645
                     /locus_tag="KLK84_002834"
     CDS             5265..5645
                     /locus_tag="KLK84_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010088.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(5700..5987)
                     /locus_tag="KLK84_002835"
     CDS             complement(5700..5987)
                     /locus_tag="KLK84_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HBE2010089.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            6116..6625
                     /locus_tag="KLK84_002836"
     CDS             6116..6625
                     /locus_tag="KLK84_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HBE2010090.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            6622..8040
                     /gene="phrB"
                     /locus_tag="KLK84_002837"
     CDS             6622..8040
                     /gene="phrB"
                     /locus_tag="KLK84_002837"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HBE2010091.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(8190..9671)
                     /gene="dtpD"
                     /locus_tag="KLK84_002838"
     CDS             complement(8190..9671)
                     /gene="dtpD"
                     /locus_tag="KLK84_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HBE2010092.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            9942..10685
                     /gene="ybgI"
                     /locus_tag="KLK84_002839"
     CDS             9942..10685
                     /gene="ybgI"
                     /locus_tag="KLK84_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HBE2010093.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10708..11364
                     /gene="pxpB"
                     /locus_tag="KLK84_002840"
     CDS             10708..11364
                     /gene="pxpB"
                     /locus_tag="KLK84_002840"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBE2010094.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            11358..11804
                     /locus_tag="KLK84_002841"
     CDS             11358..11804
                     /locus_tag="KLK84_002841"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase/urea amidolyase family protein"
                     /protein_id="HBE2010095.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFCQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGD"
     gene            11835..12290
                     /locus_tag="KLK84_002842"
     CDS             11835..12290
                     /locus_tag="KLK84_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase"
                     /protein_id="HBE2010096.1"
                     /translation="MEAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQ
                     SNRMGYRLQGQILKRITDRELLSHGLLPSVVQVPHNGQPIVLMNDAQTTGGYPRIACI
                     IEADMYHLAQIPLGQPIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            12280..13014
                     /gene="pxpA"
                     /locus_tag="KLK84_002843"
     CDS             12280..13014
                     /gene="pxpA"
                     /locus_tag="KLK84_002843"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HBE2010097.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            13050..13841
                     /gene="nei"
                     /locus_tag="KLK84_002844"
     CDS             13050..13841
                     /gene="nei"
                     /locus_tag="KLK84_002844"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HBE2010098.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(13838..14884)
                     /locus_tag="KLK84_002845"
     CDS             complement(13838..14884)
                     /locus_tag="KLK84_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBE2010099.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(15036..16112)
                     /locus_tag="KLK84_002846"
                     /pseudo
     CDS             complement(15036..16112)
                     /locus_tag="KLK84_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(16109..16840)
                     /locus_tag="KLK84_002847"
     CDS             complement(16109..16840)
                     /locus_tag="KLK84_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBE2010100.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            complement(16855..19314)
                     /locus_tag="KLK84_002848"
     CDS             complement(16855..19314)
                     /locus_tag="KLK84_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBE2010101.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            complement(19365..19931)
                     /locus_tag="KLK84_002849"
     CDS             complement(19365..19931)
                     /locus_tag="KLK84_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBE2010102.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
BASE COUNT         4951 a         4843 c         5626 g         4664 t
ORIGIN      
        1 tgttgttaat aaaaagctta tagatttata gttgataatt aaaacgcatc cgatatttca
       61 gctttttcta tgcaagatac aattttataa ggtgtccatt tttatatgga ggcggtagcg
      121 tgtctgttac tagtggtttt atttgcatag tgaatatatt gggattgtta aatacataac
      181 tatgtatccg ctctggtcgt tgtaaatatt gctaattgct taatattatt agtgattatg
      241 gctggaaaga taaagtgtgc tgtaaagcgg tgatgggtat tacattcatg attaaaaaaa
      301 cagaacctgt caataaataa ttcctttatt aaaacggact atttacatta tgacggaata
      361 ttatctcaac gaggcggttt ggaaatacag agccagtttc tccggggtaa cgagcaggtg
      421 taccagtgtc aggtggcatt tgtattgccg ggtgagcggg tcatgatggc cttcacctac
      481 gccagaacca caccgctgac cccggcagat atgacgcgct gggctgaaat taaaaagaat
      541 ctgcgcttca ggatgcggca ggaagtcagg acgaattaat catacaggga acgggacatg
      601 gaagaggcat tctgggcggc aagacagggt gatgccctgc tgcatacgtc gtttatggca
      661 gatgtgctgg gggcggtgct ggaggtggcc gcaaatgtcg ttattgatgc cctcattgtc
      721 ggcacaacct cgttactggc gacgtcagtg gggatcacga tggggtgcac ggccgtcgtg
      781 ctgaccggtt ttgtggcggg tgtggcgatg gtgtacaccg gcgtgacaga aaaggtcagc
      841 gaagcctgtt cagccctcgc aaatatgctt ttcccgcctc agattgaagg ttacatcctc
      901 accggctctg gtgacacatg gatcaacagc aaacctgccg cgcgggcagc ggcgactgcg
      961 gcatcgcggc aggacataga ggcgcaggaa gcgcaggcga aggcagaaca ggaagaagcg
     1021 cagcgtcagg aggatgccag aacgttccgg gatgtggccg gggaatatct ggaaatggcg
     1081 ggggccatag ccctggcggt taacccggtg acagggcctg tgctgatgtc ccgggctctg
     1141 cacgaccagc tcagtacgga agaagggcgt tctgaaatgg tggatggcgt gacacatttt
     1201 gtgtcggaac tatggcagcc cactgtggcc agtgcggccc cgggatccac cccgacccct
     1261 gacgataaaa tcgactgcca taaacatcct tcctcactga cgcagtttct cgcacaaaaa
     1321 aaagccgcct ttctggatga ccccctggga acggccctta acctgctgaa tccgggcggt
     1381 atgctggaaa ccgcctttca gggagtgaat gcggttattg gcagcatcag taatctgttc
     1441 aaaggcgatg acgagccgcc cgcagcggaa tatatcgcgg aaggcgcgcg ggatgtgcgt
     1501 atcaacagcc agccggcgac acgcagcggg gcacgctgta cctgcgaggc cagggtggtg
     1561 aatgagccgg ggaacggggc gtttgtgtca ccggatgtgc gcattggcgg tccgttactg
     1621 gtggtcaggg atatcagaag cggcaggtcg cagataaccc tggtggcaac cgtggcgctg
     1681 atgtttcttc gcccgggcaa aatgctttct aaaatcgcct gttttgctgc cagtgtcggg
     1741 atgagcatgc tgacgcagaa ggccaccagt gcccttccgc atccggtcaa tgccgccacc
     1801 ggggccaaat acctggccga tgacgatgac ttcgatttca gtctgccggg ccactttccg
     1861 ctggactggc agcgcgtgta cagcagtcgt gacgaacgta cggaaggaat gtttggtcag
     1921 ggctggagtg taatgtataa agtcagcctc gtgtgcacac cgggtagcgc agacgaaaac
     1981 tgcatgacct ttgtgtcggg catggggcga cgcttggata tggaagccgt actgccgggg
     2041 ggcgggtttt acagccccgg tgaagggctg gcggtaaggc gtggtgagca ggggcactgg
     2101 cttatcagca gcgatgacgg gcagtgtttt ctgtttgagg ctgacccgca ccacccgcag
     2161 cgccagcggc tgaaaatgct gggcgaccgt aacagcaact gtctgaatct gtactacgac
     2221 gaccggggcc gcattgtgga aatcagcggc gaacagcagc gtccgtgcat ccgtctgtat
     2281 tacgaactgg cggcgcatcc ccggcgggtg acacagattt atcagcattt ccctgagact
     2341 gcacctttac tgctgcgtcg ttacagctat gacgaggccg gccacctgaa cggcgtgtac
     2401 gacagcacgg ggcacctgct gcgtgagttt gcttacgacg aaaaccactg catgacgctg
     2461 caccgccagc cgggcggcga agggtattat taccagtggg gctggtatga aggtccggat
     2521 gacgcggcat ggcgggtgac ggggcaccac acggacagcg gtgaacagta ccggctggcg
     2581 tgggacctgg cgcagcgcag gctgtgcgtg acggacggac tggggcgcac acgttatcac
     2641 cagtgggatg cgcagaatca ggtgacggcg taccaggacg aagccggaca ggtgacgaca
     2701 ttccgctgga gcgacgaaga acgactgttg ctggggatga ccgaccctca gggcggcaaa
     2761 tggcgctatg tgtatgacct gcaggggcat atcacggaga cgcacgaccc gctggggcgg
     2821 gtggagcagg cgcagtggca cccggtgtgg caccagccgg aaacggaggt ggacgcggcg
     2881 ggaaacacct ggcgctgtgt gtacgatgaa cgcggcaacc ttcttgccgt gaccgacccg
     2941 ctgcagcaga acacccggta tcagtatgac caccacggct aggtggtgca gataacggat
     3001 gcgcgcgggg ggaataaata cctccagtgg aatgaagacg ggcagctcat gcggcacacg
     3061 gactgttccg gctcgcagac ggcgtggttc tacgacgaac gcacgaggct tatcagaatg
     3121 acggatgcgc agagccacag cacgcgctac gggtatgacg acagcggtca tctgacggag
     3181 gtgatactgg cggacggccg cacggtgaat taccagtcag acgcggcggg caggctggta
     3241 aagtacacca gcccgatggg gaggataacg cgctggcagc gggacgggca gggccgggtg
     3301 cgcagccgga cggacgcgat ggggcgcagg acggcattcg ggtacgacgc ttacgggcgt
     3361 ctggtcacgc tcacgaacga gaacggagaa cgctaccggt tccgccatga tgtgctggac
     3421 cgtctggcgg agcagataaa cccggacggc tgccgccagt cgtaccgcta caacgcgctg
     3481 aacgcggtca cggaagtggt gttcaccggg gaccggggcg gtgaaatccg ccaccggctg
     3541 gcgcgtgatg cggcgggccg gctcacggca aaagagacag ccgatggccg tacggaatac
     3601 gtccatgatg cggcagacca gctactggaa atccgtcgcc agcggcacga aactgacgcg
     3661 ccggaagtta tccgcttcag ctacgacaga ctggggcgga tgctgagcga ggagacggcg
     3721 cagggcatcc tgacccacca gtacgataaa ctgggcaacc ggactgccac cacctttccg
     3781 gacggcagaa cgcagcgcca tctgtactac ggcagcggcc atctccagca gataaatctg
     3841 gaccgggagg tcatcagcga gttcacgcgc gaccacctgc accgtgaagt gctcagaagc
     3901 caggggcgac tgagcacgcg gcagctttac gaccccggcg ggcggctgaa gcgcagggag
     3961 acatacagcg ggatgcgcgg ggtggtaccg gagacgttca cggaccggca gtacagctac
     4021 agtggcgagg atgaactgct gaagacgcgg cacagccggc ggggggaaac ggattacttc
     4081 tacgacccca cggggcgcat cacggcgtgt cgttcggagg atgaggggta tctggcgagc
     4141 tggcagtacg acgcggcggg aaacctgctg gggaggcgcg cgggggaaag ggccacggca
     4201 gaaaacagcg tggtgccgtt caaccggctg atgtcttacc gtggtgtgca ctaccgttat
     4261 gacgaatttg gccgtgcggt ggaaaaggag ggccggagcg gaacgcagag ttaccgttat
     4321 gatgcggaac accggatggt ggaagtgacg acagcgcggg ggacataccg gtatgtgtac
     4381 gatgcactgg ggaggcggac ggaaaaacag cacatcagcc ctgacggtaa gccgtacaac
     4441 cggacaaaat ttttatggga cggaatgcgg ctggcgcagg agagcaggcc ggagggaaca
     4501 ggcagcctgt acatctacag ggaccagggg agttatgaac cgctggcgcg ggtggataag
     4561 gcgggcaaag agggcccgaa ccggatactg tattttcaca cggatgtgaa cggcgcgccg
     4621 gaagagatga cggacagcga cgggaaaatc gtgtgggaga cggggtatca ggtctggggt
     4681 aacacgatac aggagaagga ccacggcggg gtggagcaga acctgcgcta ccagggacag
     4741 tacctcgaca gggaaacggg gctgcactac aatctgcaca ggtactatga tccggatgtc
     4801 gggcggttca tggtaactga cccgataggg ttgagaggcg gtttaaatct ctacagttat
     4861 gcacctaatc ctctaaaata tgctgatcca cttggattaa ctccatgtgc tgttagtaac
     4921 caaaaagcga atcgtttatt agatagtagt gaaaccaaag ttactgttag gtctagaagt
     4981 gacgctgaac aattgtttat ggatagatat ttaggtcata attataaaaa tatgacaggt
     5041 gagtcaggcc ccagcactaa gaacttaatg gaatatctta ctggaaataa aacaaaagca
     5101 ggttcatatc attgggatga tataaaagat ccttctgtaa caaaaccttc atatagagtg
     5161 tcaggtcatg gactaggaaa tcctgatggt gatttaccac atttacaagt tcatcaacat
     5221 ggtggaagtg ttagacatat atttttcccg tgggagacat aataatgaac aatctcaata
     5281 cattattaag tggaaaggtt ttgctcaaag aaattagtcc attaatgcct ggatatagaa
     5341 catggattga aatttcattg atcgatgaat taaaaccatc ttatcctttt agattagatg
     5401 aatatgctat gatagggcat tcaccttatg ccaatgaatg taataaagat gaagctaaat
     5461 ttaaactacg aatatcatca tttttagctt ctgacataga taatgaatac gatccatcat
     5521 atgattatgt tggaaaatat gaaattatcg cttcacttaa tgagttaaaa actaggttaa
     5581 gtactcttca tgtaaacttg gagcaattta taaattcatc ggaagatgat gagtatccat
     5641 tataataaga cagaataaaa agctaggaag gttttaaatc ttcccagctt ttttaatatt
     5701 taactttcgt tcagcacact aaacatccca tcccgcagtt cctgcacctc gtcactgtca
     5761 gcgatgatgg tcaggccccc ctccgaaatc ttcaccgtga caccccgtcc ggtatcaaat
     5821 ccggcctctt tcagccagtc gcccttaagg tgcaggctgg gatgttgtga ataataccgg
     5881 gctataccgg ttttgcggtc ctggtggcgg gctctgacat agccgacttt atagcggcgc
     5941 tgtgttttgg aaatggtgac ttctggcgta atatcgtgtt cagccatgat taactacctt
     6001 gattagttgc ttgcggggag cgcgctttcg taatcttgcc ctgctagcgg attaacttca
     6061 caaacatcat ttaccaacgc ctacacttac tcctgtatac cgttcaggag cagtaatgaa
     6121 tctacaacga tttgatgaca gcaccctaat ccgtatcttc gcccttcatg agttacatcg
     6181 actgaaagaa catggcttaa cgcgcggggc gcttctcgat tatcacagcc gctataaact
     6241 cgtctttctg gcccattctc agcccgagta ccgcaaactt ggcccgtttg tggctgatat
     6301 tcatcagtgg caaaatctgg atgattttta caaccagtac cgccaacgcg taattgtttt
     6361 gctttctcat cccgccaacc cgcgcgatca caccaacgtt ttgatgcacg ttcagggtta
     6421 ttttcgcccg catattgatt ccacagaacg ccagcagctg gctgcgctta tcgacagtta
     6481 tcgccgtggc gagcaaccac ttcttgcgcc gctgatgcgt atcaaacact atatggcgct
     6541 ttatcctgac gcctggcttt cagggcagcg ttatttcgaa ctttggccgc gtgtgattaa
     6601 cttgcgccat tcaggagttt tatgactacc catctggcct ggtttcgcca ggatttacgt
     6661 ctgcacgata atctcgcact ggctgccgcc tgccgcaatt cgtctgcacg cgtgctggcg
     6721 ctgtatatcg ctacaccacg ccagtgggag acgcataaca tgtcgccgcg tcaggctgaa
     6781 cttatcaatg ctcaactgaa tgggctacaa atcgcgctgg cggaaaaagg tattccttta
     6841 ttgttccgtg aagtggatga ctttgtcgcc agtgtcgaaa tagttaaaca ggtgtgcgcg
     6901 gaaaacagcg ttactcatct tttttataac tatcagtatg aggtgaatga gcgagcgcgg
     6961 gatgtggacg ttgaaagagc gctgcgtaac gtggtgtgtg aaggatttga tgacagcgtg
     7021 atcctgccgc cgggggcggt gatgaccggc aatcacgaga tgtacaaagt ctttacgcct
     7081 tttaagaatg cctggctgaa acggctgcgg gaagggatgc cggagtgcgt cgctgcacca
     7141 aaagttcgta gtagcggatc gataaagccc gcgccatcca ttacgctgaa ttatcctcgt
     7201 cagtctttcg atactgcgca tttcccggtg gaagaaaaag cggcgattgc gcaattacgc
     7261 cagttttgcg agaacggtgc tggagagtat gagcaacaac gagattttcc ggcagtggaa
     7321 ggcaccagcc gtttgtccgc cagcttggca acgggcgggt tatcgcctcg ccagtgtttg
     7381 catcgcttgt tggcggaaca gccgcaggcg ctggacggtg gggccggtag tgtctggctt
     7441 agtgagctga tctggcgcga gttctaccgt catctgatga cgtattaccc ctcgttgtgt
     7501 aaacatcgtc catttattgc ctggacggat cgtgtgcagt ggcaaagcaa tcccgcacat
     7561 ttacaggcct ggcagaaagg caaaacggga tacccgatta ttgatgccgc tatgcgtcag
     7621 cttaacagca ctggctggat gcataaccgg ctacggatga ttacagccag ttttctggtg
     7681 aaagatttgt tgatcgactg gcgcgaaggc gagcgatatt tcatgtcgca gctgattgat
     7741 ggtgatttgg cagctaataa cggtggctgg cagtgggcgg cttctacggg tactgatgct
     7801 gcgccgtatt ttcgtatttt caatccgata actcagggcg agaaatttga ccgtgagggc
     7861 gagtttattc gtcgatggtt accggagctg cgcgatgtac cagggaaagc ggtgcatgag
     7921 ccgtggaagt gggcgcagaa agcaggtgtg aagctggatt atccgcaacc gatagtcgag
     7981 cacaaagagg cgagagtaca aacgttggca gcgtatgagg cggcgcggaa ggggaaataa
     8041 cagacgcgtc aggcaatcga gcccagatgc cggatgcggc gtgaacgcct aatccagcct
     8101 acaaatttgt gcaaattcaa taaattgcag gaaatacgta ggcctgataa gacgcgtcaa
     8161 gcgtcgcatc aggcatcggt gctggcctat tatgactcca gcgccagcgc gcggtttctg
     8221 aatttcagcg cctgatacag ccaaatcatc agtaccagtc ctacacacgc cagtgcgccc
     8281 caggtaattt gatcaaatac ttcaatatat gcattgatgg agtagttgat cgccccggaa
     8341 gcatcaaacg aagcctgcga tgtctgatcg gcaatcacgc ccgccagata gttcgcgatc
     8401 gcgccagaaa gcagcatgta gatgccggtt aatacgccgg tcacgcccgg aatttcaatg
     8461 cgcgtaattt gcgacatggc aaccgggtcg ataaacagtt ccgcaaagcc catcaccgcg
     8521 aggcctaaca ccatcagtgg cagagaagag tgaccataca ttgcggacca gcgggcgctt
     8581 aaggtcagaa tgcagaatcc ggcgctcatc aggccaaggc caagagcaaa ttttccccag
     8641 atgcgcacgg tacgattacc tgcgacgctt tctttaacca cccacgccag gaacacaccg
     8701 cacagcataa ctgcgaaggc attaatcgac tggaacatcg cggtcggaac ggtataacca
     8761 aacatatcgc ggttaacgaa gcggtcgata taaaggctaa tcgagctacc gccctgttgt
     8821 gcgaaggccc agaacaacat actgaagaag gtgagcgtca caatcagccc cagctccttc
     8881 cgctgtttct ggttttctgc tttgcgataa atttttgcca gtacacccag accaataata
     8941 gtcgcgacaa ttaaggcgta taccgaccac tctttccaga acagcacggt aatcagtgca
     9001 ggcgttgcca ccagcagaac cagcagccat ccccagttcg gcaggagaaa gtttgtcgca
     9061 cgcagtactt ttttgttaac gccgcgggta tgagtgaaat gacgattgcc acataagaaa
     9121 atgaccagac ccgcgatcat gccaaccgcc gccaggccaa agcccatcgc ccagctgtac
     9181 tcttcctggg cgtaaccaca ggcgataggt gcgataatag atcccacgtt acccgccgca
     9241 tacatcagcg agaaaccgcc atcacgacgc ggatcggttg gctcatacag ctcgccgagc
     9301 agacagctta cgttagattt aaacaggcca tagccgcaga cgataatcgc cagggacaga
     9361 tagaggaatg acggatggat ctcactggca cccagcacca catgaccgat cgccatcaac
     9421 aacgccccca gcatcaccgc catgcgattg ccgagaactt tatccgccaa aaagccaccg
     9481 aggattggcg tgacatacac cagcgaacag taggcgctaa ataactcgta ggcgtgagta
     9541 tcgttgtatt ttagttgatt ggtgagatag agaatcagca gggcacgcat gccgtaaaag
     9601 ctaaaatatt cccagatttg cagcgcaacg acatagtaaa tagcgcgcgg ttgtgatgcg
     9661 tgtttattca taataattcc acaattgacg gcaacgtcac gtaatagttg cgcggcgtgc
     9721 ttgtgtttcc ttaaagttaa aactttgata cagatctgat tatttatgca atatgctgtc
     9781 tgattgcata aatatacatt agctgaagcg tgatgatata aacaatttgc gtcttttggc
     9841 gtcgggtttg tttcttaata tgtcaaaaga ttggctatcg acgaacaggc gatgtaacgc
     9901 tatgttaacg gtgtagctat cagaatacgg aaggcgcgaa gatgaaaaac accgaactgg
     9961 aacaactgat taacgaaaaa ctgaacagcg cggcgattag tgattacgcg ccgaatggtt
    10021 tgcaggtgga aggcaaagag acggtgcaaa aaattgttac cggtgtcacc gccagccagg
    10081 ctttgctcga tgaggcagtg cgtctgggcg cagatgcggt cattgtgcat catggctact
    10141 tctggaaagg agagtctccg gtcattcgcg gaatgaagcg taaccgttta aaaacgttgc
    10201 tggcgaatga tatcaacctg tatggctggc atttgccgct tgacgcacat cctgagctgg
    10261 gcaataacgc acagctggcg gcgttactgg ggatcacggt catgggcgaa attgagccgt
    10321 tggtgccgtg gggagagctg accatgcctg tgccgggaat ggaactagcc tcctggattg
    10381 aagcgcgtct gggacgtaag ccgttatggt gtggcgatac cggacctgag gtggtccagc
    10441 gcgtcgcctg gtgcacgggc ggcgggcaaa gttttatcga tagcgccgcg cgttttggcg
    10501 tggatgcttt tattactggc gaagtttctg aacagaccat tcattcagcc cgcgagcagg
    10561 gattgcattt ttatgctgca ggtcaccatg ccactgaacg cggtggtatt cgcgcattga
    10621 gcgagtggct gaatgaaaat accgatcttg atgtgacctt tattgatatt cctaatcctg
    10681 cataacgaat aatcagaggg atcgaaagtg caacgagcgc gttgttatct gataggtgaa
    10741 acggcggtag tgctggaact ggaaccgccg gtgacgctgg ctagccagaa acggatctgg
    10801 cgactggcgc agcgtctggt ggatatgccg aatgtggttg aagccattcc cggcatgaac
    10861 aatatcacgg tgattttgcg taatcctgag tcgctggcgc tggatgccat agagcgtttg
    10921 caacgctggt gggaggagag cgaggccctg gaacctgagt ctcgctttat tgaaattccg
    10981 gtggtttacg gtggtgcagg cggaccggat ttggcggtgg tcgcggcgta ttgcgggttg
    11041 agcgaaaaac aggttgttga attgcactcc tccgtggaat acgtggtctg gtttttaggt
    11101 tttcaaccgg gcttcccgta tctcgggagt ttgccggaac aactacacac gccacggcgc
    11161 gctgaaccgc gcttgctcgt tccggcaggt tctgtcggga tcggcgggcc gcagactggt
    11221 gtttatccgc tggcaacgcc gggtggctgg cagttgattg gtcatacctc actcagcctg
    11281 tttgatccgg cgcgtgacga acccatctta ttacgtccgg gagacagcgt gcgatttgta
    11341 ccgcagaagg agggaatatg ctgaagatta ttcgtgcggg catgtacacc actgtgcagg
    11401 atggcggtcg tcacggtttt tgccagtcgg gtatcagcca ctgtggcgca ctggatatgc
    11461 ctgcgctgcg cattgctaac ctgctggtgg gtaatgacgc caatgccccc gcgctggaga
    11521 tcacgctcgg tcagttaaca gttgagttcg aaactgatgg gtggtttgcc ctgacgggtg
    11581 caggttgcga agcgcggttg gatgataacg ccgtctggac cggctggcga ttgccgatga
    11641 aagcaggcca gcgtttaacg cttaaacgcc cgcagcacgg gatgcgcagt tatctggcgg
    11701 tcgcgggtgg tattgatgtt ccgccggtaa tggggtcatg cagcaccgat ctcaaagtgg
    11761 ggattggcgg gctggaaggc cgtttactga aggatggtga ctgactcccg attggcaaat
    11821 cgaagcgtga ttttatggaa gcgcagggcg ttaaacagct gctgtggggc aaccgcattc
    11881 gcgccttgcc ggggccggaa tatcatgagt tcgatcgcgc ctcgcaggat gcattctggc
    11941 gttcgccctg gcagcttagc tcgcaaagta accgaatggg ctatcgttta caggggcaaa
    12001 ttttaaaacg catcaccgat cgcgaactgt tatctcacgg tttgttaccg agcgtggtgc
    12061 aggtgccgca taacgggcag ccgattgtgt tgatgaacga cgcacagacc accggtggtt
    12121 atccacgtat tgcctgtatc attgaggctg atatgtacca tctggcgcaa attccgctcg
    12181 gtcagccgat tcattttgtc cagtgttcac tggaagaggc actgaaagcg cggcaagatc
    12241 agcaacgtta ttttgaacaa ttagcgtggc ggctgcacaa tgaaaattga cctgaacgcc
    12301 gatctgggcg aaggctgcgc cagcgacgca gagctattaa cgctggtttc ctctgccaat
    12361 attgcttgtg gatttcatgc aggcgatgcg caaaccatgc aggcttgcgt gcgtgaagca
    12421 ataaaaaatg gtgtcgcgat tggcgctcac ccgagttttc ccgacaggga aaattttggt
    12481 cgcagcgcca tgcagctgcc gccggaaacc gtttacgccc agacgctgta tcaaattggc
    12541 gcgctggcaa cgattgcccg tgcgcaaggc ggcgtaatgc gtcatgtcaa accgcacggc
    12601 atgttgtaca accaggcggc gaaagaagca caactggcag acgccatcgc cagagcggta
    12661 tacgcttgcg atccggcatt ggttctcgtt gggctggcag gaagcgagct gattcgtgca
    12721 ggcaagcaat atggcctgac aacgcgcgag gaagtgtttg ccgatcgcgg ttatcaggct
    12781 gacggctcgc tggtgccgcg aagtcagcca ggcgcgttga ttgaaaacga agaacaggcg
    12841 ttggcgcaaa cgctggagat ggtgcaacac ggcagagtca aaagtatcac cggcgaatgg
    12901 gcaacggtcg cggcgcaaac ggtctgcctg catggcgacg gcgagcacgc tctggcgttc
    12961 gcccgccgat tacgagctac atttgccaaa aaggggattg ttgtcgcagc ataaccccgc
    13021 ttaataaaga atgaaaaaag gatatcacca tgcctgaagg cccggagatc cgccgtgcag
    13081 cggataacct ggaggcggcg atcaaaggca aaccactaac tgatgtctgg tttgccttcc
    13141 cgcagttaaa aacttatcaa tcacaactta tcggtcaaca cgttacccat gtggaaacgc
    13201 gtggtaaggc gttgttaact cattttccca acggtttaac gctctacagc cataatcagc
    13261 tttacggcgt ctggcgcgtg gttgataccg gcgaagagcc gcagaccacg cgagtattgc
    13321 gggtaaaact gcaaacggct gacaaaacca ttctgcttta tagcgcctcg gatattgaga
    13381 tgttgaggcc ggaacaactg accacgcatc cgtttttaca acgcgttggt cccgatgtgc
    13441 tggatccgaa tctgacgccg gaggtggtaa aggaacggtt attgtcgccg cgctttcgta
    13501 accgtcagtt tgcaggatta ctgctcgatc aggcgtttct ggctgggctt ggcaattatt
    13561 tgcgggtgga gatcctctgg caggttgggt tgactggaaa tcataaagcg aaagatctca
    13621 atgcggcgca actggatgca ctcgcacacg cgttactgga gattcctcgg ttttcttacg
    13681 ctacgcgggg gcaggtggat gagaataagc atcatggggc gctgtttcgc tttaaggttt
    13741 ttcatcgtga tggcgaactg tgcgaacgct gtggcggcat cattgagaaa accacgttgt
    13801 cctcgcgccc gttttactgg tgtccaggct gccagcacta ggccgaccgc ttcggcgcat
    13861 aggttgaaat aaaccgcgca atggctggcc ccgtcagcaa aatactgaac aggcgcaggg
    13921 tttgcatcgc cataataagc gccatatcgg cattgctccc tgcggcgatg accgccacgg
    13981 tatcaagccc gccgggactg gtggcgaggt aggcagtcat aaaatcaata tgcataaacc
    14041 gggtcagtcc ccacgccata cctgcacaaa tagccagcag agcaaaaatc gacagcagga
    14101 tttgcggtag cgggcgtagt gcccgcagta agatttgttt atcgaaacca agaccaatcc
    14161 gccagccaat tgccatatac gccatcgcca gcagccattc cggtagttcg atggtgatga
    14221 gctgaccaga ctggagcact gcgcccgcca gcatcgggat gagcatcgtg ccggaaggaa
    14281 gacgcagcat gcatcccgcc gtaccggcaa cgactgcgag caaaatcgta agcaggagat
    14341 taatgctcac tggcgggaac cagacgattt gctggttaac cgcttcagcg ttatcaccca
    14401 gcatcatgcg ggtgaccaga acagcggccc ccgcgacgaa cagcactcgc agatattgca
    14461 taaacgccac caggcgaata tctgcgccgt aatcttgcgc catagcgacc attgccgctg
    14521 cgccgcctgg agaagaaccc caggcaccgg tatttccagg cagtgagcta tagcgcacca
    14581 ataaccaacc cacgatggcg ctggaaagca gcgtcaccag taaaatagca agcacgatcg
    14641 gccagttggc ggccagcgtg gtgagaatcg aaccagtgag gttttgcgca atcatgcagc
    14701 caagaatggc ctgcgcggcg agaaaagcgg agcggggaag ttgcagagta attccgcgca
    14761 tactgaagat gatcccggcg atcatcggcc caagtaatag cgccgccggg agatgtaccg
    14821 cgaggaaacc aatagaaagg agaagtgata gcacacataa cattccccac tgcaaaactg
    14881 gcatccttcg ctccctcatt attttctctt tggtgtatcg atacagcata aagaaagccg
    14941 ggctaggaaa aaataacagc aatcaaggaa aaggggaaaa tcagcaattt tctgaaagag
    15001 atgccctttc cggctgcaaa gggcatcatg gcaaatcagt tatagttaat tttaaaaagc
    15061 accactgtat cgaagggccc agcctcgatg ggggtacctg caatggacga aagttcagct
    15121 gtataggttt tttcgtagac caacatgtcg ctggtgaaat cggcgtattc agcatattta
    15181 ttgaaggtat aaggcgaggc gttttcatcc agcaaacgta gcttcagacc attaccaatc
    15241 aacaatgctt tatcttcttc aaccaacgtt tcttcggtat aaaaggagga actgacttta
    15301 aatccgtcgg tgcattggtc attttgtgat tttgtcgtct taatactgaa tgttttcgac
    15361 attgtgtggc gacgaatatc cagcaaatta aatttaccaa aatcaatcat ctgtgtttcc
    15421 ggtgaaatgg aaaaattgac gctgcaatcc agcacgcgaa tgttttccag accagtaata
    15481 tgatatttca ggttttgggc agtagggtct tcgttaacgc taccggcacc gtcaaattgc
    15541 acgacgatat agtcgctaag cgtactttga taatcatgcg gcggcatttc tcgtattttg
    15601 acgtataacc gcatgcgagc gagaaaggtt cgcgacatgt gaatattagc gggatcaccc
    15661 gagcaaattt tgttctgcca tcccatcagg ggtataaatg tcaatgttct tactgtcgat
    15721 acactggtta gtgtcgatgc ggctttttcc ctggttggcg tcataatcaa cgccattgta
    15781 agttactccg agttgataat aacgatcctg cacccccggg taggggttta cccatgcgta
    15841 gacatgttcg ctttcaaagt tgccgttggt attgttgtca caatagacgg gaattttaat
    15901 atcgtcagat tcccagatct tattttaata tcgtcagatt cccagatctt atcgccgagt
    15961 ttggcattgc ctggtacagc aaaaggttga atcgcttctg atttttctac cgagccaccg
    16021 gatgaaccaa agtagcagtt cagggcaagc gcagacttca acgacagaaa taacagacag
    16081 atgacgagca acactccctt gccagcactc atttcttcgc ctccacggct tgtaaatggc
    16141 attgtccgct gcagttgagc gtcgtcatgc gtaacgcgcc gtaatcatcc atataaccga
    16201 gataaaactg gctgccgcta taatttccgg tgtttgtgtt gaccgtacta aaaggcgcaa
    16261 ccatcacggt tttgaagccg gggagaacgc ctttttcatt tcgtcccagg taggcaatgg
    16321 tcagataata cgcggtagga tttttcagtg tcagttggtt gccctgctgg ctgacctgta
    16381 actgcagttc gaccttttct cctgctttct tgcgtaatgc agccggtcgc cagaacacct
    16441 tgatacgact ctgaatggcg acttgcagaa cggcatggtc gctgtttttt tccggggcgg
    16501 gaggaatttc acgcatattg tagaaaaata gcgtttcgcg atcgcccggt aattttgtgg
    16561 tcgaggcttg ctttactatc cgtacctgtg ttgttgcttt cggttccaga cgttgtatcg
    16621 gcggcagggc gaccagaaga tcatcgcttt tctccccttt ttcattttcg agccaggaat
    16681 aagcgaggta ggggagttta tcgctacgat tatccacccg cagactggta gctttatcat
    16741 tagcgttaaa cacaatacga gtgcgatcgg gttgtagtgc agcgtggctg cacagactgg
    16801 caaccaacaa caaaaggggt agtcctttca taaatgtcat tttcatttcc ttaattaatg
    16861 acaaggtaaa atcaggcgat ttgcggtgtc ttccatatgt tcaggaagat ggagcgagca
    16921 atgctggcta tcaccccaga ccacggtaaa cttttgattt tcagcgactc cgcttaacca
    16981 ggcatgtcct tcctcgccaa ccatccccac gctaatgccg ctgtcatcct ggcggatatc
    17041 cgctccgagg ggaggaaact gaccgctggc gttgcgaatg atgacgttaa cgtctttacc
    17101 ggaacgggaa gccagtgatt tgtaacctat cgcgccttca atccacgttt ctttgataac
    17161 gttttctgca actgttacgc cgtcgggtaa gtcattcatg ttcaccgcca cggtggaagg
    17221 ctggtaactg gaaatcaacg gcaccaccgc aatgccaaaa tggttggtgt agtcgagatt
    17281 gccctgaacc ggaatatctg ccacgccatc ggtgctgacc atcaggcgtg gttcattggt
    17341 ggagctgcgg cgatgaaacg ctgcaccata ttgggttgcg gtgaaagaac cgctccagct
    17401 gctgctgacg gaactgtaat cattggcggc ataggtacca gaaatatccc actcacccgc
    17461 tgaactcagg tgctgatagt taccgctcac ctgggctcca ttgtcaggac ggtcggattg
    17521 cagtccggca gacatgcccc agctattacg ttcatccagc gtatcgttcc acgacatgcg
    17581 gtgtgtggtg ctgtggctac tgttttgcat gtcatagcca acccgaccac cgttaccgaa
    17641 ggggagcgaa atcgacaggt aaatctggtt gtcgcgatct ttatcttcgt aatgggttgt
    17701 attgaatgac gtagaaatag agatatctct ccagtcacca atatcaacat taaaacctgc
    17761 cgtgatgttg gcggtcgtgg aggcgtctgc attccaccag gtttgatgta gcaggttggc
    17821 gtaaagattg aggtttagtg gggtaatcgg ttggcccaca gataagctga tcgtctgttt
    17881 ttcgtcctgc gcatcgctgt cgttgtattt gtgatccaga tagttggcgt agctgtgaaa
    17941 atgacgatcg gagaaacgat aagcggcgag ggaaatcgtg ctgttagtgg catccacttg
    18001 tttgctgtaa ttaaaacggt agcttaagcc ccgctcgtcc tgctgagtat caaaatggct
    18061 actggcccac gtgacatcaa acgacagcgc accaagccac agcatatttt gcccaatacc
    18121 cattgcggca gaatggtagt catcaccaga aagcagcagg ccgctgtaca gcgaggtgtt
    18181 tgacagcatc ccccaggaaa cttcattgct aaaaaaggtt tcattttcag tttgatgtga
    18241 catggagggc cgtggctgac ccgcggccag tttatagcga acctgtccct gacgagtcag
    18301 gaagggcgtc gatgctgctg aaacctggaa attgttcacc cgaccatctt cttccgtcac
    18361 tttgacatcc agtgtgccct gaacagactg attaaggtcg tcaatgataa atgggcctgg
    18421 tgggactttt ttctggtaaa tgacgcggcc tgattgactg atcgtcaccg tggcattggt
    18481 ctgtgcaata ccgctaattt gtggggcgta gccgcgtagt tcccatggca acattcgatc
    18541 gtcacttgcc agtgccgcgc cggtataaga aaaaccgtcg aaaatattgg gactaaaatc
    18601 cgtttcgccg agggttaatt ttgaacctaa ttgcggtaac gggcgaaaaa gataggtacg
    18661 tgatatttcg cctgactgtt catggttatc atcgctatca gtatgattca actgataatc
    18721 actgcgtaag cgccatgccc ccgtgttgat tccggctgtt ccataggcgt taagattagt
    18781 gctgctgctg ccatcctgtg ggcggtagct gctggcaaac aagttgtaat ccatcaggac
    18841 accggcgaca ccttctttcc atgtggaggg tggggtccag ttttctgagt gccacgccag
    18901 ccaggcttgc ggaatactaa tattcagttg ctgattggct tgatcgaaat tgaagagcat
    18961 ttcaggtcgg gagctaaaat cgacgcactg atttatcaat ggtaacgact gacggacttc
    19021 aggttttaag ccaaatttat cgaccagtaa atcattgatg cacggaatgg ttttatcgtc
    19081 atttttgtgc cagttaatct tttgctcgtt actgatttgg ttattattta ccgcaacgct
    19141 aacaaaatac tcaccgggag caatgactcc tttttccttt aataatgaaa tatcaatgcg
    19201 gtcgcgcatt gatttatcga gaacattcag attgaattcg accgccaatg cgcaaggcat
    19261 cgccacgacc aggcaggata caaaagagag tcgataaata ttcacggtgt ccatacctga
    19321 taaatatttt atgaaaggcg gcgatagcgc cgccaaagta atacttattt ataatccagc
    19381 acgtaggttg cattcgccgt aactgcgcct ggcgttacag gtgtagcctg atcaatttgt
    19441 tccatccagg caaagaaatt cagcgtttgt tcgctggagg tcggagccag gtcgatatct
    19501 ggagtagcgg tgcctaaaac gatattgctt tgatctttat tcattaaacg tacgccgacg
    19561 ccggtggctt cacccgtgct ggtgttgttg agaatcggat ctgcgccggt ggttttcgct
    19621 gagctatcaa aggtgactgc aactttagaa atcttgccac cggaaccgtt gctggagttt
    19681 tccagatcac agttgactaa gagcaggtcg acagcaacgg catcgctgtg atgtgactga
    19741 ttaatgtggc tggtggtcac ctgaccgagt tcaacctctt tatcaatatc atcctgatgt
    19801 atttcacacg gtgcttcaat aacttcacct ttaaagtgaa tttcaccgga tccttcatca
    19861 gccgcataaa caggtgctgt gaacagcaga gatacggcca gtgcggccaa tgttttttgt
    19921 cctttaaaca taactgagtc ctttaacaga aaagaaaaaa gtatatcggt gcaagaatat
    19981 cttatttgat tgtcgaatgt ttttagtttt tcttaaaaat attattaatt ttattaaatg
    20041 taaaagtaat taattgatgg tatgaattaa atgtttgatg aata
//
LOCUS       DAEEUE010000076        19414 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.076, whole genome shotgun sequence.
ACCESSION   DAEEUE010000076 DAEEUE010000000
VERSION     DAEEUE010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19414
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.076"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            111..2201
                     /gene="pbpC"
                     /locus_tag="KLK84_002850"
                     /pseudo
     CDS             111..2201
                     /gene="pbpC"
                     /locus_tag="KLK84_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; incomplete; partial
                     in the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
     gene            2351..2782
                     /gene="ndk"
                     /locus_tag="KLK84_002851"
     CDS             2351..2782
                     /gene="ndk"
                     /locus_tag="KLK84_002851"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000963836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBE2010103.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTC"
     gene            2932..4086
                     /gene="trmG/rlmN"
                     /locus_tag="KLK84_002852"
     CDS             2932..4086
                     /gene="trmG/rlmN"
                     /locus_tag="KLK84_002852"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HBE2010104.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4371..5384
                     /gene="rodZ"
                     /locus_tag="KLK84_002853"
     CDS             4371..5384
                     /gene="rodZ"
                     /locus_tag="KLK84_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HBE2010105.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGVWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5411..6538
                     /gene="ispG"
                     /locus_tag="KLK84_002854"
                     /gene_synonym="gcpE"
     CDS             5411..6538
                     /gene="ispG"
                     /locus_tag="KLK84_002854"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBE2010106.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6641..7915
                     /gene="hisS"
                     /locus_tag="KLK84_002855"
     CDS             6641..7915
                     /gene="hisS"
                     /locus_tag="KLK84_002855"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBE2010107.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7933..8553
                     /gene="yfgM"
                     /locus_tag="KLK84_002856"
     CDS             7933..8553
                     /gene="yfgM"
                     /locus_tag="KLK84_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HBE2010108.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8564..9742
                     /gene="bamB"
                     /locus_tag="KLK84_002857"
     CDS             8564..9742
                     /gene="bamB"
                     /locus_tag="KLK84_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HBE2010109.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9860..11332
                     /gene="der"
                     /locus_tag="KLK84_002858"
     CDS             9860..11332
                     /gene="der"
                     /locus_tag="KLK84_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBE2010110.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11402..11617
                     /locus_tag="KLK84_002859"
     CDS             11402..11617
                     /locus_tag="KLK84_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBE2010111.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11614..12984)
                     /gene="xseA"
                     /locus_tag="KLK84_002860"
     CDS             complement(11614..12984)
                     /gene="xseA"
                     /locus_tag="KLK84_002860"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBE2010112.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13146..14612
                     /gene="guaB"
                     /locus_tag="KLK84_002861"
     CDS             13146..14612
                     /gene="guaB"
                     /locus_tag="KLK84_002861"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBE2010113.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14681..16258
                     /gene="guaA"
                     /locus_tag="KLK84_002862"
     CDS             14681..16258
                     /gene="guaA"
                     /locus_tag="KLK84_002862"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBE2010114.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16353..16892)
                     /locus_tag="KLK84_002863"
     CDS             complement(16353..16892)
                     /locus_tag="KLK84_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HBE2010115.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16908..17426)
                     /locus_tag="KLK84_002864"
     CDS             complement(16908..17426)
                     /locus_tag="KLK84_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBE2010116.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17737..17928)
                     /locus_tag="KLK84_002865"
     CDS             complement(17737..17928)
                     /locus_tag="KLK84_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HBE2010117.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17946..18098
                     /locus_tag="KLK84_002866"
     CDS             17946..18098
                     /locus_tag="KLK84_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010118.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            18280..>19414
                     /gene="pdeF"
                     /locus_tag="KLK84_002867"
     CDS             18280..>19414
                     /gene="pdeF"
                     /locus_tag="KLK84_002867"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HBE2010119.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQ"
BASE COUNT         4568 a         4730 c         5278 g         4838 t
ORIGIN      
        1 gcttatcgcc ctggcgagac gacgctgcga cgttctgttc gccatgatgc gcgacaggac
       61 tttttatacc ccgcaggggt cataacatgc ttgacaactt aataggggca cgttaccttg
      121 aagcgctgat caattatgaa gatcgctggt tctggaagca tccgggggtt aatccattct
      181 cggtggcgcg cgcagcatgg caagatctca cttcgggacg ggttatttcc ggtggcagca
      241 cgctcactat gcaggttgct cgtctgcttg atcctcaccc caaaacattt ggtggcaaaa
      301 ttctccagct ctggcgcgcg ttgcaactgg agtggcattt gtctaagcgt gaaattctga
      361 tcttgtatct taaccgcgct ccgtttggcg gtacgttgca ggggatcggt gcggcaagtt
      421 gggcttatct cggaaaatcg cctgcgaatt taagctattc cgaggcggca atgctggcgg
      481 tgttaccgca agtgcccagc cgactgcgcc cggatcgttg gccggagcgt gccgaagccg
      541 cgcgtaataa agtgctcgaa cggatggctg cgcaaggtgt gtggtcccgt gagcaggtaa
      601 aagagtcaag ggaagaaccc atctggctgg caccccgaca aatgccgcaa ctggcaccgc
      661 tgttttcgcg catgatgctc ggtaaaagca aaagcgacaa aatcgttact acgctggatg
      721 ccggtcttca acgacgtctg gaagaactgg cgcaaaactg gaaagggcgg ttgccaccgc
      781 gcagctcact ggcgatgatc gtggttaatc ataccgatat gcgtgttcgc ggctgggtgg
      841 gatcggttga tctcaacgat gattcacgct ttggtcatgt tgatatggtc aatgcgatcc
      901 gatcgccagg atcggtgctc aaaccgtttg tttatggtct ggcgctggat gaaggcttga
      961 tccacccggc atcactgctg caagacgtcc cccggcgcac cggtgattat cgaccaggta
     1021 actttgatag cggttttcat ggcccgatca acatgagcga ggcgctggtg cgctcgctga
     1081 acttacctgc tgtgcaggtg ctggaagcct atggaccgaa acggtttgcg gcaaagttac
     1141 gcaatgttgg attgccgtta tatttgccca acggcgctgc gccgaatctt tcactcattc
     1201 tcggcggcgc tggtgcaaaa ctggaagata tggcggcagc gtataccgcg tttgctcgcc
     1261 acggcaaggc aggcaaattg cgcttacagc ctgacgatcc gctgcttgaa cggcctttaa
     1321 tgtcgtcggg ggcggcgtgg atcattcgcc ggattatggc tgatgaagcg caacccttgc
     1381 cggatagtgc cttgccgcgc gtcgccccac tggcatggaa aacgggcacc agctatggct
     1441 atcgtgacgc ctgggcgatt ggggttaacg ctcgctatgt cattgggatc tggactggca
     1501 gacctgacgg cacgcccgtt gttggtcagt ttggctttgc cagtgccgta ccattgttga
     1561 atcaggtcaa taatatctta ctgtcgcgca gtgcgaatct gccggaagac ccgcgcccga
     1621 actcagtcac tcgtggcgtt atctgctggc cgggcggaca gtctttgccg gaaggtgacg
     1681 gtaactgtcg ccgccgcctg gcaacctggc tgctggatgg cagccagccg ccaacgctgt
     1741 tactgtcaga gcaggaaggg attaatggca ttcgcttccc catctggctg gatgacaaag
     1801 ggcagcgcgt cgccgctgac tgcccgcagg cgaagcaaga aatggtaaat gtctggccgc
     1861 taccgctgga accctggctg cccgcatcag aacgtcgcgc tgtgcgctta ccaccggcct
     1921 cgacaatttg cccgccatac ggtcacgatg cccagctccc tctgcagctg acaggcgttc
     1981 gcgatggcgc gattattaaa cgtttaccgg gcgcagcgga agcgactttg ccgttgcaat
     2041 caagtggagg ggcaggtgaa cgctggtggt ttctgaatgg cgaaccgtta actgaacgcg
     2101 ggcgcaacgt gaccctgcat ttgacggata aaggtgatta tcaattgctg gtgatggatg
     2161 atgtggggca aatcgcgaca gtgaaatttg tcatgcaata gtctgttttg tccctgattg
     2221 ttgctaaaac tcattttatt ttaaaaaaaa tgttaccttc atcaatagtc aacggccctg
     2281 ttgctcatta taatccgcgc catctcgtac gctggtacag acaacaacag aacaatttac
     2341 agaggtaaaa atggctattg aacgtacttt ttccatcatc aaaccgaacg cggtagcaaa
     2401 aaacgtcatt ggtaatatct ttgcgcgctt tgaagctgca gggttcaaaa ttgtcggcac
     2461 caaaatgctg catctgaccg ttgaacaggc acgtggcttt tatgctgaac acgatggaaa
     2521 accgttcttt gatggtctgg ttgaattcat gacctctggc ccgatcgtgg tttccgtgct
     2581 ggaaggtgaa aacgccgttc agcgtcaccg cgatctgctg ggcgcgacca atccggcaaa
     2641 cgcactggct ggtactctgc gcgctgatta cgctgacagc ctgaccgaaa acggtaccca
     2701 cggttctgat tccgtcgaat ctgccgctcg cgaaatcgct tatttcttcg gcgaaggcga
     2761 agtgtgcccg cgcacctgtt aataatttcg taaatgccgc gtgcaaacgt ggcatccgtg
     2821 cgccagaatt tgtacaatgc agcgcccccg gacgagcagc cgctcaccgg ggcgtttctt
     2881 ttttcaaccc tccaggggcc ataacgtgta ataacgaggc cggaatagca tatgtctgaa
     2941 caattagtca cacctgaaaa cgtcaccacg aaagatggaa aaatcaacct gctggatctc
     3001 aaccgtcagc agatgcggga gttttttaaa gatttaggtg aaaaaacctt ccgcgccgat
     3061 caggtgatga agtggatgta tcactattgc tgcgacaact ttgatgagat gaccgacatc
     3121 aacaaagtgt tgcgcggcaa actgaaagag gtggcggaaa tccgcgcacc ggaagtggtt
     3181 gaagaacagc gttcatccga cggcaccatt aaatgggcga tcgccgttgg cgatcagcgc
     3241 gtcgaaacgg tgtatatccc ggaagacgac cgtgccacgc tctgcgtctc ttcgcaggtg
     3301 gggtgtgcgc tggagtgtaa attctgttcc accgcccagc agggctttaa ccgcaacctg
     3361 cgggtatcgg aaattattgg ccaggtgtgg cgtgcggcga aaatcgtcgg cgcagcaaaa
     3421 gtcaccggtc agcgcccaat cactaacgtc gtgatgatgg gcatgggcga gccgctgctc
     3481 aacctgaaca acgtcgttcc ggcgatggaa atcatgcttg atgatttcgg ttttggcctg
     3541 tctaaacgtc gcgtgacgct ttccacttcc ggtgtagttc cggcgctgga taaactgggc
     3601 gatatgatcg acgttgcact ggcaatttcc ctgcacgcgc cgaacgacga aattcgtgac
     3661 gaaattgtgc cgatcaacaa aaagtacaat atcgaaacgt tccttgctgc ggtgcgccgt
     3721 tatctggaga aatccaacgc caatcagggc cgagtcacta ttgaatacgt gatgcttgac
     3781 cacgttaacg acggcactga acacgcgcac caactggcgg aactgctgaa agatacgccg
     3841 tgtaagatca acctgatccc gtggaacccg ttcccggacg cgccgtatgg acgcagttcg
     3901 aacagccgta tcgaccgttt ctcaaaagtg ctgatgagct acggtttcac cactattgtg
     3961 cgtaaaacgc gtggtgatga tatcgatgct gcctgtggtc agttggcggg cgatgttatc
     4021 gaccgtacga aacgtactct gcgtaaacgg atgcagggtg aagccatcga tattaaagcg
     4081 gtctgatagc cattacgcca cggttacttt atgtaccgtg gcgtaatctt ttatgaatga
     4141 agtattaagt tgcaattgtc tggtcaatat taacggtgcg tttttgctga ctttaaggca
     4201 gtatgtaacg atgcaacagt aagttagcct cagtgaatgt gggctttgtc acgagcacac
     4261 agacggtctt atactgtatg ataacggtta acttaacgga tgtttcgcgg tgtgggtgag
     4321 catgatggtt caccggcatc tcaattctca tttaaacgta cctgcagcga atgaatactg
     4381 aagccacgca cgaccaaaat gaagcactta ctaccggcgc tcgcctgcgt aatgctcgcg
     4441 aacaactagg acttagtcag caggccgttg ccgagcgact ttgcctgaag gtttccacgg
     4501 tacgcgacat tgaagaagat aaggcacccg ccgatcttgc ttcaacattc ctgcgcggat
     4561 atatccgctc ttatgcgcgt ctggtacata ttccagaaga agaactgctg ccagggctgg
     4621 aaaagcaggc tccacttcgg gctgcaaaag ttgcgccaat gcagagtttt tccctcggta
     4681 aacgccgcaa aaaacgcgac ggctggctaa tgaccttcac ctggctggtg ttgtttgtgg
     4741 ttatcggcct gagcggtgtc tggtggtggc aagaccacaa agctcagcag gaagagatca
     4801 ccactatggc cgatcaatct tcggcggaac tgagcagtaa tagcgagcag gggcagagtg
     4861 ttccgttaaa tacgtcgaca actacagacc cggctacaac gtccacgccg ccagcgtctg
     4921 tggatactac cgcaaccaac acgcaaacac ctgccgtaac tgcgccagca ccagctgttg
     4981 atccgcaaca gaatgcggtt gtttcgcctt cgcaggcaaa tgttgatacc gccgcgaccc
     5041 cggtaccaac ggcagcaaca acgccagatg gtgctgcgcc gttgccaacc gatcaggctg
     5101 gcgtgaccac gccggcggct gatccgaatg cgctggtgat gaactttact gccgattgct
     5161 ggctggaggt cactgatgct accggtaaaa aattgtttag cgggatgcag cgtaaagacg
     5221 gtaatttgaa tttaaccggc caggcaccgt ataaactgaa aattggtgcg ccagccgcag
     5281 tacagatcca gtatcaaggg aaacctgtcg atctgagtcg ttttatcaga actaaccagg
     5341 ttgcgcgtct gaccctcaat gccgaacaat caccggcgca gtaacagacg ggtaacgcgg
     5401 gagatttttc atgcataacc aggctccaat tcaacgtaga aaatcaacac gtatttacgt
     5461 tgggaatgtg ccgattggcg atggtgctcc catcgccgta cagtccatga ccaatacgcg
     5521 tacgacagat gtcgaagcaa cggtcaatca aatcaaggcg ctggaacgcg ttggcgctga
     5581 tatcgtccgt gtctccgtac cgacgatgga cgcggcagaa gcgttcaaac tcatcaaaca
     5641 gcgggttaac gtgccgctgg tggctgacat ccacttcgac tatcgcattg cgctgaaagt
     5701 agcggaatac ggcgtcgatt gtctgcgtat taaccctggt aatatcggta atgaagagcg
     5761 tattcgcatg gtggttgact gtgcgcgcga taaaaacatt ccgatccgta ttggcgttaa
     5821 cgccggatcg ctggaaaaag atctgcaaga aaagtatggc gaaccgacgc cgcaggcgtt
     5881 gctggaatcc gccatgcgtc atgttgatca tctcgatcgc ctgaacttcg atcagttcaa
     5941 agtcagcgtg aaagcgtctg acgtcttcct cgctgttgag tcttatcgtt tgctggcaaa
     6001 acagatcgat cagccgctgc atctggggat caccgaagcc ggtggtgcac gcagcggggc
     6061 agtaaaatcc gccattggtt taggtctgct gctgtcggaa ggcatcggcg acacgctgcg
     6121 tgtatcgctg gcggccgatc cggtcgaaga gatcaaagtc ggtttcgata ttttgaaatc
     6181 gctgcgtatc cgttcgcgag ggattaactt catcgcctgc ccgacctgtt cgcgtcagga
     6241 atttgatgtt atcggtacgg ttaacgcgct ggagcaacgc ctggaagata tcatcactcc
     6301 gatggacgtt tcgattatcg gctgcgtggt gaatggccca ggtgaggcgc tggtttctac
     6361 actcggcgtc accggcggca acaagaaaag cggcctctat gaagatggcg tgcgcaaaga
     6421 ccgtctggac aacaacgata tgatcgacca gctggaagca cgcattcgtg cgaaagccag
     6481 tcagctggac gaagcgcgtc gaattgacgt tcagcaggtt ggaaaaataa taacgtgatg
     6541 ggaagcgcct cgcttcccgt gtatgattga acccgcatgg ctcccgaaac attgagggaa
     6601 gcgttgaggg ttcattttta tattcagaaa gagaataaac gtggcaaaaa acattcaagc
     6661 cattcgcggc atgaacgatt acctgcctgg cgaaacggcc atctggcagc gcattgaagg
     6721 cacactgaaa aacgtgctcg gcagctacgg ttacagtgaa atccgcttgc cgattgtaga
     6781 gcagaccccg ctattcaaac gtgcgattgg tgaagtcacc gacgtggttg aaaaagagat
     6841 gtacaccttt gaggatcgca atggcgacag cctgactctg cgccctgaag ggacggcggg
     6901 ctgtgtacgc gccggcatcg agcatggtct tctgtacaat caggaacagc gtctgtggta
     6961 tatcgggccg atgttccgtc acgagcgtcc gcagaaaggg cgttatcgtc agttccatca
     7021 gttgggctgc gaagttttcg gtctgcaagg tccggatatc gacgctgaac tgattatgct
     7081 cactgcccgc tggtggcgtg cgctgggtat cttcgaacac gtaactcttg agctgaattc
     7141 tatcggttcg ctggaagcac gcgccaatta ccgcgatgcg ctggtggcat tccttgagca
     7201 gcataaagaa aagctggacg aagactgcaa acgccgcatg tacactaacc cactgcgcgt
     7261 gctggattca aaaaatccgg aagtgcaggc gcttctcaac gacgctccgg cattaggtga
     7321 ttatctggac gaggaatctc gtgagcattt tgccggtctg tgcaaactgc tggagagcgc
     7381 ggggatcgct tacaccgtaa accagcgtct ggtgcgtggt ctggattact ataaccgtac
     7441 cgttttcgag tgggtgacta acagtctcgg ctcccagggc accgtgtgtg caggcggtcg
     7501 ttatgacggt cttgtggaac aactgggcgg tcgtgcaaca ccggctgtcg gttttgcgat
     7561 gggcctcgaa cgtcttgtat tgttagtaca ggccgttaat ccggaattta aagccgatcc
     7621 tgttgtcgat atatacctgg tggcttcagg tgctgataca caatctgcgg ctatggcatt
     7681 agctgagcgt ctgcgtgatg aattactggg cgtgaaattg atgaccaacc acggcggcgg
     7741 caactttaag aaacagtttg cccgtgctga taaatggggt gcccgcgttg ctgtggtgct
     7801 gggtgagtct gaagtggcta acggcacagc agtagtgaag gatttgcgct ctggtgagca
     7861 aacggcagtt gcgcaggata gcgtagccgc gcatttgcgc acgttactgg gttaaggaag
     7921 gagaaggaca gcgtggaaat ttacgagaac gaaaacgacc aggtagaagc ggttaaacgc
     7981 ttttttgctg aaaatggcaa agcactggct gttgggggga ttttgggcgt tggcgcactg
     8041 attggctggc gctactggaa cagccatcag gttgattctg cacgctccgc ttctcttgcc
     8101 tatcaaaatg cggttaccgc agtgagcgaa ggcaaaccgg atagcatccc ggcggcggaa
     8161 aaatttgctg ctgaaaataa aaatacttat ggtgcgctgg cttctttgga acttgcgcag
     8221 caatttgttg acaaaaatga actagagaaa gctgccgccc agttacaaca ggggctggca
     8281 gacacgagcg atgaaaatct caaagccgtg ataaatctgc gtcttgctcg cgttcaggta
     8341 cagctcaagc aggctgatgc cgcgctgaaa acccttgata ccatcaaagg tgaagggtgg
     8401 gctgccattg ttgccgactt gcgtggtgaa gcattgctga gcaaaggtga taagcaaggt
     8461 gcgcgtagtg catgggaagc aggcgtgaaa agcgatgtta ctccggcact gagcgaaatg
     8521 atgcagatga aaattaataa tttgtccatc tgagagggac ccgatgcaat tgcgtaaatt
     8581 actgctgcca ggactgcttt ccgttaccct tttaagcggc tgttcgctgt ttaacagcga
     8641 agaagatgtg gtaaagatgt ccccattgcc aaccgttgaa aaccagttta cgccgaccac
     8701 ggcgtggagc acttccgttg gtagcggcat tggcaacttc tattccaatc ttcatccggc
     8761 actggcggac aacgttgtct atgcagcgga ccgcgctggt ttggtaaaag cgctgaatgc
     8821 ggatgatggc aaagaaatct ggtctgtcag cctggccgag aaagatggct ggttctctaa
     8881 agagcctgca ttactttctg gcggtgtgac cgtgtctggt gggcatgtct acattggcag
     8941 cgaaaaggcg caggtttacg cgctgaatac cagcgatggt actgtggcat ggcaaactaa
     9001 agtcgcgggt gaagcacttt cgcgcccggt ggtcagcgac ggtctggtgt taatccacac
     9061 cagtaacggt cagttacaag cgctgaacga agctgacggc gctgtcaaat ggacagttaa
     9121 cctcgatatg ccttcgctct ctttgcgtgg cgagtctgcg ccggcaacgg cttttggtgc
     9181 ggccgtcgtg ggtggcgata atggtcgcgt cagcgcagtg ctgatggaac agggccagat
     9241 gatttggcag cagcgtattt cccaggcgac cggttctacc gaaattgacc gtctgagcga
     9301 tgttgacacg actcccgtcg ttgttaacgg cgttgttttc gcgctggcct ataatggtaa
     9361 cctgacggcg cttgatctgc gcagtggtca gattatgtgg aaacgcgaac tgggttcggt
     9421 gaatgatttc atcgtcgatg gcaatcgcat ctatctggtc gatcaaaatg accgggtgat
     9481 ggcgttgacc attgatggcg gcgttacgct gtgggcacaa agcgatctgc tgcatcgcct
     9541 gctgacttct ccggtgctgt ataatggcaa cctggtggtc ggtgacagtg aaggttatct
     9601 gcactggatt aacgtcgaag atggtcgttt cgttgcccag caaaaagttg atagttccgg
     9661 tttccagact gaaccggttg ccgctgacgg caaactgctg atccaggcaa aagacggaac
     9721 cgtgtactct attacacgtt aatcgtctct gtcgttcact ttgaaaacgg ctcctggaca
     9781 ggggccgttt tcctgttttt aacaacgacg cgaatatagc gtgcgttgtc tgatgattta
     9841 taaaaatgag gctttaaaca tggtacctgt ggtcgcgctt gtcgggcgcc ctaacgtagg
     9901 aaaatccacg ttatttaacc gtctaactcg cacccgagat gcgctggttg cggatttccc
     9961 gggtctgact cgtgaccgta agtacggtcg tgcggaaatt gaaggccgtg agtttatctg
    10021 tattgatacc ggcgggattg atggcacaga agacggtgta gaaacccgca tggcggaaca
    10081 gtcgctgctg gcgattgaag aagcggacgt cgtactgttt atggtggatg cgcgcgcggg
    10141 cctgatgccg gcagatgaag cgattgccaa acatctgcgc tcccgtgaaa aaccgacctt
    10201 cctggtggta aacaaaactg acggtctgga tcccgatcag gcagtggttg atttctactc
    10261 gcttggttta ggtgaaatct acccgatcgc cgcgtctcac ggtcgtggcg tattaagtct
    10321 gctggagcat gtgctgctgc cgtggatgga agatctcgca ccgcaagagg aagtcgacga
    10381 agacgctgaa tactgggcgc aatttgaagc ggaagagaac ggcgaagaag aagaggaaga
    10441 cgacttcgac ccgcaaagtc tgccgatcaa actggcgatt gtgggtcgtc cgaacgtagg
    10501 taagtctaca ctcactaacc gtattcttgg agaagagcgc gttgttgttt acgacatgcc
    10561 tggcacgacg cgtgacagca tctacatccc aatggaacgc gatggacgtg agtatgtgct
    10621 cattgacacc gctggcgtac gtaaacgcgg caaaatcacc gatgctgtag agaaattctc
    10681 cgtaatcaaa acgttgcagg ccattgaaga cgccaacgtg gtgatgttag tgattgatgc
    10741 gcgcgaaggt atttccgatc aggatctctc gctgctgggc tttattctca atagtgggcg
    10801 ctcacttgtc attgtggtga ataagtggga tggcctgagc caggaagtga aagagcaggt
    10861 gaaagaaacg ctggacttcc gtctgggctt tatcgatttt gctcgtgtgc actttatctc
    10921 tgccttgcac ggcagtggtg ttggtaactt gtttgaatca gtacgtgaag cgtatgacag
    10981 ctccacccgt cgtgtgggga cctctatgct gacgcgcatc atgacgatgg ctgttgaaga
    11041 tcaccaaccg ccgctggtac gcggtcgtcg tgtgaagctg aaatatgccc acgctggtgg
    11101 ttataacccg ccgattgtgg tgattcacgg taatcaggtg aaagacctgc ctgattccta
    11161 caagcgctac ttgatgaact acttccgcaa atcgctggac gtaatgggat cgccgattcg
    11221 tattcagttc aaagaagggg aaaacccgta tgcgaacaag cgtaacaccc tgacgccaac
    11281 ccagatgcgt aaacgtaagc gtctgatgaa gcacatcaag aaaaataaat aattatccgt
    11341 aagtatttta tctgccattc cctctatgaa tgtagaggga atggctaccg gaggaattca
    11401 tatggaactg cattgtccaa aatgtcagca tgtgcttgat caggataacg gccatgcgcg
    11461 ttgcccatcc tgcggggaat ttattgaaat gaaagctctt tgtccagact gccatcaacc
    11521 gttgcaggtg ttaaaagcct gcggtgcagt agattacttc tgtcagcacg ggcatggctt
    11581 gatatcgaaa aaacgcgttg aattcgtgct ggcttaatga acctttttac gcgatttttt
    11641 taccggctgg atgtttttta cttcactttc tatccagccg tcttccagac gtgtggttag
    11701 catttcaccc gttttaactt gcttcacttt tttcagtaca ttgccgtcag tagcagtagt
    11761 aacgctgtat ccacgcgcca gcgttgacag tgggcttacg gcttcgaggt gcgttactgc
    11821 attaccgaaa cgttcacgcg tggcgctaag ctgtgcgcgc aggatttctg ctaaacgata
    11881 ttccagttgc tgaatgcgcg tttgcgtgcg atgaatcttc ggctgtggat tttgctgatt
    11941 cagccgctgt gttaatcgct gctgctgttg cccggcacgc ttaagctgat tttccagcgc
    12001 aaagctcatc cgtttttgca ggcgttcaag catggtttgc tggcgtgcca gccggagctg
    12061 cggatgctgc tgctgcaagc gatgatgaat ctgcgtaaag cgacgtgtac ggttggcgag
    12121 ataataatcc atcgccatct ccagccgttg acgggccgat tgcacctggc gcagtaactc
    12181 ttgctgatta cggctcacta cttcagcggc ggcagacggc gttggcgcac gcagatcggc
    12241 aacaaaatcg gcaatggtca catccgtctc atgcccgacg gcgctgacga ccggaatgcg
    12301 gctggcaaaa atcgcccgcg ctacgcgttc gtcgttaaaa ctccataaat cttccagcga
    12361 accgccgccg cgcccaacga tcaacacgtc gcactcattg cgctgattcg ccagttcaat
    12421 ggcgcgaaca atttgccccg gcgcgtcatc gccctgaacg gaggtggggt agatgatcac
    12481 cggtagagaa ggatcgcgac gttttaacac atgcaaaata tcatgtagcg cagcaccggt
    12541 ttttgaggtg atcacaccaa cgcaatgcgc aggggaggga agtggttttt tgtattgctg
    12601 atcgaacaaa ccttcagcct gcaacttcgc tttgagctgt tcgtacttct gttgcagcag
    12661 cccttcaccg gccggctgca tactctcaac gattatctgg tagtcgccgc gcggctcgta
    12721 gagcgtaata ttggcgcgaa ctaaaacttg ttgcccatgc tgcggacgga aggtgacccg
    12781 gcggttgctg ttgcggaaca tcgcgcagcg tacctgggcg gtgtcgtctt tgagtgtaaa
    12841 gtaccagtga ccggaagccg gttgtgtgaa attagagatt tcgccgctga tccaaacctg
    12901 tcccatctca tgctcaagca gcagacgaac cgtttgattc aggcgactaa cggtaaaaat
    12961 tgcaggggat tgagaaggta acatgtgagc gagatcaaat tctaaatcag caggttattc
    13021 agtcgatagt aacccgccct tcggggatag caagcatttt ttgcaaaaag gggtagatgc
    13081 aatcggttac gctctgtata atgccgcggc aatatttatt aaccactctg gtcgagatat
    13141 tgcccatgct acgtatcgct aaagaagctc tgacgtttga cgacgttctc ctcgttcctg
    13201 ctcattctac cgttctgccg aatactgctg acctcagcac ccagctgacg aaaactattc
    13261 gtctgaatat ccctatgctt tccgcagcaa tggataccgt aacggaagcg cgcctggcta
    13321 ttgctctggc tcaggaaggc ggtatcggct ttatccacaa aaacatgtcc attgaacgcc
    13381 aggcagaaga agttcgccgt gtgaaaaaac acgaatctgg tgtggtgact gatccgcaga
    13441 ctgttctgcc aaccacgacg ctgcgcgaag tgaaagaact gaccgagcgt aacggttttg
    13501 cgggctatcc ggtcgttacc gaagaaaacg aactggtggg tattatcacc ggtcgtgacg
    13561 tgcgttttgt taccgacctg aaccagccgg ttagcgttta catgacgccg aaagagcgtc
    13621 tggtcaccgt gcgtgaaggt gaagcccgtg aagtggtgct ggcaaaaatg cacgaaaaac
    13681 gcgttgaaaa agcgctggtg gttgatgacg aattccacct gatcggcatg atcaccgtga
    13741 aagacttcca gaaagcggaa cgtaaaccga acgcctgtaa agacgagcaa ggccgtctgc
    13801 gtgttggtgc tgcagttggc gcaggtgcgg gtaacgaaga gcgtgttgac gcgctggttg
    13861 ccgctggcgt tgacgttctg ctgatcgact cctcccacgg tcactcagaa ggcgttttgc
    13921 agcgtatccg tgaaacccgt gctaaatatc cggatctgca aatcatcggc ggcaacgtgg
    13981 caacagctgc aggtgcacgc gctttggcag aagctggttg cagcgcggtt aaagtcggta
    14041 tcggccctgg ttctatctgt acgactcgta tcgttactgg cgtaggtgtt ccgcagatca
    14101 ctgccgttgc tgacgcagtt gaagccctgg aaggcaccgg tattccggtt atcgcagatg
    14161 gtggtattcg cttctccggc gacatcgcca aagctatcgc cgctggcgca agcgcggtga
    14221 tggtaggttc catgctggcg ggtactgaag aatctccggg tgaaatcgaa ctctaccagg
    14281 gccgttctta taaatcttac cgtggtatgg gttccctggg cgcgatgtcc aaaggttcct
    14341 ctgaccgtta tttccagagc gataacgctg ccgacaaact ggtgccggaa ggtatcgaag
    14401 gtcgcgtagc ttataaaggt cgcctgaaag agatcattca ccagcagatg ggcggcctgc
    14461 gctcctgtat gggtctgacc ggctgtggta ctatcgacga actgcgtact aaagcggagt
    14521 ttgtacgtat cagcggtgcg ggcattcagg aaagccacgt tcacgacgtg accattacta
    14581 aagagtcccc gaactaccgt ctgggctcct gattctcttc gcccgatttc atgtcgggcg
    14641 atttatatta tctgtttcac ttgcctcgga ataagcgtca atgacggaaa acattcataa
    14701 gcatcgcatc ctcattctgg acttcggttc tcagtacact caactggttg cgcgccgcgt
    14761 gcgtgagctg ggtgtttact gcgaactgtg ggcgtgggat gtgacagaag cacaaattcg
    14821 tgacttcaat ccaagcggaa ttattctttc cggcggcccg gaaagtacta ctgaagaaaa
    14881 cagtccgcgt gcgccgcagt atgtctttga agcaggcgta ccgatattcg gcgtttgcta
    14941 tggcatgcag accatggcaa tgcagttggg cggtcacgtt gaagcctcta acgaacgtga
    15001 atttggctac gcgcaggtag aagtcgtaaa cgacagcgca ctggttcgcg gtatcgaaga
    15061 tgcgctgacc gcagacggta aaccgctgct cgatgtctgg atgagccacg gcgataaagt
    15121 taccgctatc ccgtccgact tcgtcaccgt agccagcacc gaaagctgcc cgtttgccat
    15181 tatggctaac gaagaaaaac gcttctatgg cgtacagttc cacccggaag tgactcacac
    15241 ccgccagggt atgcgcatgc tggagcgttt tgtgcgtgat atctgccagt gtgaagccct
    15301 gtggacgcca gcgaaaatta tcgacgatgc tgtagctcgc atccgcgagc aggtaggcga
    15361 cgataaagtc atcctcggcc tctctggtgg tgtggattcc tccgtaaccg caatgctgct
    15421 gcaccgtgct atcggtaaaa acctgacttg cgtattcgtc gacaacggcc tgctgcgtct
    15481 caacgaagca gagcaggttc tggatatgtt tggcgatcac tttggtctta acattgttca
    15541 cgtaccggca gaagatcgct tcctgtcagc gctggctggc gaaaacgatc cggaagcaaa
    15601 acgtaaaatc atcgggcgcg ttttcgttga agtattcgat gaagaagcgc tgaaactgga
    15661 agacgtgaag tggctggcgc agggcaccat ctaccctgac gttatcgaat ctgcggcttc
    15721 tgcaaccggt aaagcacacg tcatcaaatc tcaccacaac gtgggcggcc tgccgaaaga
    15781 gatgaagatg ggcctggttg aaccgctgaa agagctgttc aaagacgaag tgcgtaagat
    15841 tggtctggag ctgggcctgc cgtacgacat gctgtaccgt catccgttcc cgggaccagg
    15901 ccttggcgtt cgtgttctgg gtgaagtgaa gaaagagtac tgtgacctgc tgcgccgtgc
    15961 tgacgccatc ttcattgaag aactgcgtaa agcggacctg tacgacaaag tcagccaggc
    16021 gttcaccgtc ttcctgccag tacgttccgt tggcgtaatg ggcgatggtc gtaagtatga
    16081 ctgggttgtc tctctgcgtg ctgtcgaaac catcgacttt atgaccgcac actgggcgca
    16141 tctgccgtac gatttcctcg gtcgcgtttc caaccgcatt atcaatgaag tgaacggtat
    16201 ttcccgcgtg gtgtatgaca tcagcggcaa gccgccagct accattgagt gggaatgatt
    16261 ttgatccctg cactatgaat gaacaaaacc ctccgttact acagagggtt ttttatcttc
    16321 aagaatcata ggcttgaagt tactaacatc gattaattaa accagctgtc cgatttgttc
    16381 tcttctgctt tgcccacgct tttcatcaga tcgcgaccgc cttcagtcat atttctgttt
    16441 gcgtcagctt cagattgcac cacatcggtt tgcgcagctt tgtgcttcag ttcctgatcg
    16501 ataaattcgt tttcgcgctt aacgcgggct tcttctttcg ccagcgccag tttttgtttc
    16561 tgaatctcta agctgcgtag ctcatcttca taactttgat cgcgtttttt gtccgcagtg
    16621 gcttcggcgt ccagtttatc ctgacgagct ttcttatttg ccgctgccgt tgccgctctt
    16681 ttgttcgcgg cggcctgggc atttgcgcga cgttgcttct cttgctggat ttccctgttg
    16741 cgctccgcga cccattcgtc atgctgcctt tgctcttcat ttttaccttg ctgttccgct
    16801 tctgcgacag cagagagttg atcctgcaat gatgaggcta tagccggata gcttaaggag
    16861 gctaagatgg cgcaaagaaa aactttcttc atgactcctc ctgattatta gctcttttca
    16921 ggacatttgg tatttggctg aatacgcgtt tcgttatacg ttgtggtaat aacaacggct
    16981 aaacctgtcg taaactggca ctctttaccc tcctgggtgg aggtatacac tttggtgcct
    17041 tccttatatg ttaaagaaac accttccact aaggttttat cattcaccat agaacccgct
    17101 gccgcgccta cagctccgcc gccaactgcc cctgccgtcg ttccggaatt gctgccagaa
    17161 ccgacgttgt ggccgataac accgccagcg actgcgccaa taagcgcgcc gaaggcttgt
    17221 gcgttccgtt tattttggga gttgtctacg gcaacttttg cgggaagaat ggaaataata
    17281 ttaacggttt tagtttcttg tttggtattc agttgatcgg tttgataaac atcggcggca
    17341 tgatcgtcag catttgactg gcatcctgcc agagtgaatg acgctaacat tgccacaggc
    17401 agaagacatt ttttaaattt catcactatt ccttgttata cacattacga aaaataagac
    17461 atttaagatc atcaaaagag gaaagtaaat gtattgcgta taaagggata gattaaattt
    17521 attaaaattc acgcagggaa taatttataa aaatatatca ttacaacata tatattaatt
    17581 ttatatttat ttcgcagcca aacaggataa acatcagtca ggacaaagac gtaaaattta
    17641 agatgaaaga atatctgccg ctttgataat cccaaagagc aaaagcatcc gacagcgaag
    17701 tgccgttatg ttctgtgaaa gtggagaagg taaccgctaa cgttgtaccg gtgattcgac
    17761 ggagagtgtg gattgctgag cttctttttt gctctgatgg tgctgccagg caccgacgga
    17821 cgagtagata aaacggccaa agaagaagat aaagctgatg agcagtacga tacgggtcat
    17881 gcgactgtta aatcggtgtc gttttcgcat actagttgcc tgactcacaa aaggttcctt
    17941 gaagtatgtc ccacgctgtg gacggtactt acattaaggc acaacaggac aaaatggtca
    18001 attcttcgtt atgtaaaaaa gcgtcagtgg atacatattt taatgttatg gaagttaatt
    18061 taattattta caatgatgat gtaataatga tgaattaaca tgataaacgt aataattcat
    18121 taatcagggt tattttgttt gatatatatc aattggaact ttcatatgac acttagaatc
    18181 aatgctctct atagtgatga ataatcatca ttcgaagtca ggtgggatgc ctgtctgaat
    18241 acacctcctt caggatgtgg gggattctgc tgagcatcta tgaaactgaa tgcaacttat
    18301 ataaaaatac gtgataaatg gtgggggctt ccgctgttcc tgccttcttt aatcttgccc
    18361 attttcgccc acattaatac tttcgcgcat atttcttccg gtgaggtttt tctcttttat
    18421 ctgcctttgg cactgatgat cagcatgatg atgtttttca gctgggcggc attgccaggg
    18481 atcaccttag ggatttttgt ccgcaaatat gcagagctgg gtttttacga aacgctctca
    18541 ttaacggcta attttattat cattatcatt ctctgttggg gcggttacag ggtctttact
    18601 ccccggcgta acaacgtttc acatggtgat acccgtttaa tttcccagcg tatattctgg
    18661 cagatagtgt ttcctgcaac gctgtttctg atacttttcc agtttgctgc atttgtagga
    18721 ttactggcga gcagagaaaa tctggtcggt gtcatgcctt ttaacctcgg gactttaatc
    18781 aattatcagg ccttactggt gggtaatctg atcggtgtcc cgctgtgcta cttcatcatt
    18841 cgggtagtgc gaaatccatt ttatttacgt agctattatt cgcaattaaa acagcaggtt
    18901 gatgccaaag tcaccaaaaa agagttcgcg ctctggctac tggcattagg tgctttattg
    18961 ttgctgttat gcatgccgtt aaatgaaaaa agcacaattt ttagcaccaa ttataccttg
    19021 tcattattgc tgcccctgat gatgtgggga gcaatgcgct atggctataa gctgatttcg
    19081 ctgctctggg cggtcgtgtt gatgatcagc atccacagct atcaaaatta cattcccatt
    19141 tatcctggct ataccacgca gctgaccata acctcctcca gttatctggt attctctttt
    19201 attgtcaatt atatggctgt actggcaact cgtcagcgag cggtagtcag acgcattcag
    19261 cggcttgcgt atgtggaccc ggtggttcat ctgccaaatg ttcgcgccct gaatcgcgcg
    19321 ttgcgtgatg ccccctggtc tgcgctttgt tatttacgca tccctggcat ggaaatgctg
    19381 gttaagaact atggcatcat gctgcggatt caat
//
LOCUS       DAEEUE010000077        19375 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.077, whole genome shotgun sequence.
ACCESSION   DAEEUE010000077 DAEEUE010000000
VERSION     DAEEUE010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19375
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.077"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="KLK84_002868"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="KLK84_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HBE2010120.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="KLK84_002869"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="KLK84_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HBE2010121.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDAQVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVLERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="KLK84_002870"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="KLK84_002870"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBE2010122.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="KLK84_002871"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="KLK84_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HBE2010123.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="KLK84_002872"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="KLK84_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HBE2010124.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="KLK84_002873"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="KLK84_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBE2010125.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="KLK84_002874"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="KLK84_002874"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBE2010126.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9127
                     /gene="atpI"
                     /locus_tag="KLK84_002875"
     CDS             8747..9127
                     /gene="atpI"
                     /locus_tag="KLK84_002875"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HBE2010127.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9136..9951
                     /gene="atpB"
                     /locus_tag="KLK84_002876"
     CDS             9136..9951
                     /gene="atpB"
                     /locus_tag="KLK84_002876"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBE2010128.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9998..10237
                     /gene="atpE"
                     /locus_tag="KLK84_002877"
     CDS             9998..10237
                     /gene="atpE"
                     /locus_tag="KLK84_002877"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HBE2010129.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10299..10769
                     /gene="atpF"
                     /locus_tag="KLK84_002878"
     CDS             10299..10769
                     /gene="atpF"
                     /locus_tag="KLK84_002878"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBE2010130.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10784..11317
                     /gene="atpH"
                     /locus_tag="KLK84_002879"
     CDS             10784..11317
                     /gene="atpH"
                     /locus_tag="KLK84_002879"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBE2010131.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11330..12871
                     /gene="atpA"
                     /locus_tag="KLK84_002880"
     CDS             11330..12871
                     /gene="atpA"
                     /locus_tag="KLK84_002880"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBE2010132.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12922..13785
                     /gene="atpG"
                     /locus_tag="KLK84_002881"
     CDS             12922..13785
                     /gene="atpG"
                     /locus_tag="KLK84_002881"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBE2010133.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13812..15194
                     /gene="atpD"
                     /locus_tag="KLK84_002882"
     CDS             13812..15194
                     /gene="atpD"
                     /locus_tag="KLK84_002882"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBE2010134.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15215..15634
                     /gene="atpC"
                     /locus_tag="KLK84_002883"
     CDS             15215..15634
                     /gene="atpC"
                     /locus_tag="KLK84_002883"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBE2010135.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15985..17355
                     /gene="glmU"
                     /locus_tag="KLK84_002884"
     CDS             15985..17355
                     /gene="glmU"
                     /locus_tag="KLK84_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBE2010136.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17517..19346
                     /gene="glmS"
                     /locus_tag="KLK84_002885"
     CDS             17517..19346
                     /gene="glmS"
                     /locus_tag="KLK84_002885"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBE2010137.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
BASE COUNT         4768 a         4858 c         5122 g         4627 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttatgctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccaagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgctcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggcg caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaagtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gggtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac
     8941 accagcgaaa ggccgggtgg cttggacatt cgcatttggc gaagctttca aagttctggc
     9001 gatgttggtg ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat
     9061 cgttacgtgg gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa
     9121 agggtaaaag gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac
     9181 ctgaataacc ttcagctgga cctgcgtaca ttctcgctgg tggatccaca aaacccccca
     9241 gccaccttct ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg
     9301 ttcctggttt tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt
     9361 cagaccgcga ttgagctggt gatcggcttt gttaatggta gcgtgaaaga catgtaccat
     9421 ggcaaaagca agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg
     9481 aatctgatgg atttactgcc tatcgacttg ctgccgtaca ttgctgaaca tgtactgggt
     9541 ctgcctgcac tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg
     9601 ggcgtattta tcctgattct gttctacaac atcaaaatga aaggcatcgg cggcttcacg
     9661 aaagagttga cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt
     9721 gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg
     9781 tatgccggtg agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg
     9841 atcctgaatg tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc
     9901 ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac
     9961 actactacgt tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct
    10021 gctgtacatg gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat
    10081 cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct
    10141 gctgcgtact cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt
    10201 aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga
    10261 gcaatatcag aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc
    10321 tcggccaggc catcgcgttt gtcctgttcg ttctgttctg catgaagtac gtatggccgc
    10381 cattaatggc agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt gcttccgcag
    10441 aacgagcaca taaggacctt gaccttgcaa aggccagcgc gaccgaccag ctgaaaaaag
    10501 cgaaagcgga agcccaggta atcatcgagc aggcgaacaa acgccgctcg cagattctgg
    10561 acgaagcgaa agctgaggca gaacaggaac gtactaaaat cgtggcccag gcgcaggcag
    10621 aaattgaagc cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt gctatcctgg
    10681 ctgttgctgg cgccgagaag atcatcgaac gttccgtgga tgaagctgct aacagcgaca
    10741 tcgtggataa acttgtcgct gaactgtaag gagggagggg ctgatgtctg aatttattac
    10801 ggtagctcgc ccctacgcca aagcagcttt tgactttgcc gtcgaacacc aaagtgtaga
    10861 acgctggcag gacatgctga cgtttgccgc cgaggtaacc aaaaacgaac aaatggcaga
    10921 gcttctctct ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg cagtttgtgg
    10981 tgagcaactg gacgaaaacg gtcagaacct gattcgggta atggctgaaa atggtcgtct
    11041 taacgcgctc ccggatgttc tggagcagtt tattcacctt cgtgccgtga gtgaggctac
    11101 cgctgaggta gacgtcattt ccgctgccgc actgagtgaa caacagctcg cgaaaatttc
    11161 tgctgcgatg gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa tcgataagtc
    11221 tgtaatggca ggcgttatca tccgagcggg tgatatggtc attgatggca gcgtacgcgg
    11281 tcgtcttgag cgccttgcag acgtcttgca gtcttaaggg gactggagca tgcaactgaa
    11341 ttccaccgaa atcagcgaac tgatcaagca gcgcattgct cagttcaatg ttgtgagtga
    11401 agctcacaac gaaggtacta ttgtttctgt aagtgacggt gttatccgca ttcacggcct
    11461 ggccgattgt atgcagggtg aaatgatctc cctgccgggt aaccgttacg ctatcgcact
    11521 gaacctcgag cgcgactctg taggtgcggt tgttatgggt ccgtacgctg accttgccga
    11581 aggcatgaaa gttaagtgta ctggccgtat cctggaagtt ccggttggcc gtggcctgct
    11641 gggccgtgtg gttaacactc tgggtgcacc aatcgacggt aaaggtccgc tggatcacga
    11701 cggcttctct gctgtagaag caatcgctcc gggcgttatc gaacgtcagt ccgtagatca
    11761 gccggtacag accggttata aagccgttga ctccatgatc ccaatcggtc gtggtcagcg
    11821 tgaattgatc atcggtgacc gtcagacagg taaaaccgca ctggctatcg atgccatcat
    11881 caaccagcgc gattccggta tcaaatgtat ctatgtcgct atcggccaga aagcgtccac
    11941 catttctaac gtggtacgta aactggaaga gcacggcgca ctggctaaca ccatcgttgt
    12001 ggtagcaacc gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg ccggttgcgc
    12061 aatgggcgaa tacttccgtg accgcggtga agatgcgctg atcatttacg atgacctgtc
    12121 taaacaggct gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc caggacgtga
    12181 agcattcccg ggcgacgttt tctacctcca ctctcgtctg ctggagcgtg ctgcacgtgt
    12241 taacgccgaa tacgttgaag ccttcaccaa aggtgaagtg aaagggaaaa ccggttctct
    12301 gaccgcactg ccgattatcg aaactcaggc gggtgacgtt tctgcgttcg ttccgaccaa
    12361 cgtaatctcc attaccgatg gtcagatctt cctggaaacc aacctgttca acgccggtat
    12421 tcgtcctgcg gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag cacagaccaa
    12481 gatcatgaaa aaactgtccg gtggtatccg taccgctctg gcacagtatc gtgaactggc
    12541 agcgttctct cagtttgcat ccgaccttga cgatgcaaca cgtaagcagc ttgaccacgg
    12601 tcagaaagtg accgaactgc tgaaacagaa acagtatgcg ccgatgtccg ttgcgcagca
    12661 gtctctggtt ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac tgtcgaaaat
    12721 tggcagcttc gaagccgctc tgctggctta cgtcgaccgt gatcacgctc cgttgatgca
    12781 agagatcaac cagaccggtg gctacaacga cgaaatcgaa ggcaagctga aaggcatcct
    12841 cgattccttc aaagcaaccc aatcctggta acgtctggcg gcttgcctta gggcaggccg
    12901 caaggcattg aggagaagct catggccggc gcaaaagaga tacgtagtaa gatcgcaagc
    12961 gtccagaaca cgcaaaagat cactaaagcg atggagatgg tcgccgcttc caaaatgcgt
    13021 aaatcgcagg atcgcatggc ggccagccgt ccttatgcag aaaccatgcg caaagtgatt
    13081 ggtcaccttg cacacggtaa tctggaatat aagcaccctt acctggaaga ccgcgacgtt
    13141 aaacgcgtgg gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg tttgaacatt
    13201 aacctgttca aaaaactgct ggcggaaatg aagacctgga ccgacaaagg cgttcaatgc
    13261 gacctcgcaa tgatcggctc gaaaggcgtg tcgttcttca actccgtggg cggcaatgtt
    13321 gttgcccagg tcaccggcat gggggataac ccttccctgt ccgaactgat cggtccggta
    13381 aaagtgatgt tgcaggccta cgacgaaggc cgtctggaca agctttacat tgtcagcaac
    13441 aaatttatta acaccatgtc tcaggttccg accatcagcc agctgctgcc gttaccggca
    13501 tcagatgatg atgatctgaa acataaatcc tgggattacc tgtacgaacc cgatccgaag
    13561 gcgttgctgg ataccctgct gcgtcgttat gtcgaatctc aggtttatca gggcgtggtt
    13621 gaaaacctgg ccagcgagca ggccgcccgt atggtggcga tgaaagccgc gaccgacaat
    13681 ggcggcagcc tgattaaaga gctgcagttg gtatacaaca aagctcgtca ggccagcatt
    13741 actcaggaac tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg ttatttcgta
    13801 gaggatttaa gatggctact ggaaagattg tccaggtaat cggcgctgta gttgacgtcg
    13861 aattccctca ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa aatggtaatg
    13921 agcgtctggt gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt accatcgcaa
    13981 tgggttcctc cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa cacccgatcg
    14041 aagtcccggt aggtaaagcg actctgggcc gtatcatgaa cgtactgggt gaaccggtcg
    14101 acatgaaagg cgagatcggt gaagaagagc gttgggcgat tcaccgcgca gcaccttcct
    14161 acgaagagct gtcaaactct caggaactgc tggaaaccgg tatcaaagtt atcgacctga
    14221 tgtgtccgtt cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt gtaggtaaaa
    14281 ccgtaaacat gatggagctc attcgtaaca tcgcgatcga gcactccggt tactctgtgt
    14341 ttgcgggcgt aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa atgaccgact
    14401 ccaacgttat cgacaaagta tccctggtgt atggccagat gaacgagccg ccgggaaacc
    14461 gtctgcgcgt tgctctgacc ggtctgacca tggctgagaa attccgtgac gaaggtcgtg
    14521 acgttctgct gttcgttgac aacatctatc gttacaccct ggccggtacg gaagtatccg
    14581 cactgctggg ccgtatgcct tcagcggtag gttatcagcc gaccctggcg gaagagatgg
    14641 gcgttctgca ggaacgtatc acctccacca aaactggttc tatcacctcc gtacaggcgg
    14701 tatacgtacc tgcggatgac ttgactgacc cgtctccggc aaccaccttt gcgcaccttg
    14761 acgcaaccgt ggtactgagc cgtcagatcg cgtctctggg tatctacccg gccgttgacc
    14821 cgctggactc caccagccgt cagctggacc cgctggtggt tggtcaggaa cactacgaca
    14881 ccgcgcgtgg cgttcagtcc atcctgcaac gttatcagga actgaaagac atcatcgcca
    14941 tcctgggtat ggatgaactg tctgaagaag acaaactggt ggtagcgcgt gctcgtaaga
    15001 tccagcgctt cctgtcccag ccgttcttcg tggcagaagt attcaccggt tctccgggta
    15061 aatacgtctc cctgaaagac accatccgtg gctttaaagg catcatggaa ggcgaatacg
    15121 atcacctgcc ggagcaggcg ttctacatgg tcggttccat cgaagaagct gtggaaaaag
    15181 ccaaaaaact ttaacgcctt aatcggaggg tgatatggca atgacttacc acctggacgt
    15241 cgtcagcgca gagcaacaaa tgttctctgg tctggtcgag aaaatccagg taacgggtag
    15301 cgaaggtgaa ctggggatct accctggcca cgcaccgctg ctcaccgcca ttaagcctgg
    15361 tatgattcgc atcgtgaaac agcacggtca cgaagagttt atctatctgt ctggcggcat
    15421 tcttgaagtg cagcctggca acgtgaccgt tctggccgac accgcaattc gcggccagga
    15481 tctcgacgaa gcgcgagcca tggaagcgaa acgtaaggct gaagagcaca ttagcagctc
    15541 tcacggcgac gtagattacg ctcaggcgtc tgcggaactg gccaaagcga tcgcgcagct
    15601 gcgcgttatc gagttgacca aaaaagcgat gtaacaccgg cttgaaaagc acaaaagcca
    15661 gtctggaaac aggctggctt tttttacgcg ttgacccgtc ctgaagagcg ttcacataga
    15721 tcctgctgat ataaaacacc cctgttttcc cgtttattca ttgatcgaaa taagagcaaa
    15781 aacatccacc tgacgcttaa atgaaggtac tgccttaatt ttctgcagac aaaaggcgtg
    15841 acgatggtcg aaaatggcac tttcgtcagc ggggataatc cgttattgaa caatttatcc
    15901 tctgtccatt tcacgatgaa aaaaatgtag ttttttcaag gtgaagcggt ttaaattcgt
    15961 tctcaaatta cagtcaggac gcgtatgttg aataatgcta tgagcgtagt gatccttgcc
    16021 gcaggcaaag gcacgcgcat gtattccgat cttccgaaag tgctgcatac ccttgccggg
    16081 aaagcgatgg ttcagcatgt cattgatgct gcgaatgaat taggcgcagc gcacgttcac
    16141 ctggtgtacg gtcacggcgg cgatctgctt aaacaggcgc tgaaagacga caaccttaac
    16201 tgggtgcttc aggcagagca gctgggtacg ggtcatgcaa tgcagcaggc cgcacctttc
    16261 tttgccgatg atgaagacat tttaatgctc tacggcgacg tgccgctgat ctctgtcgaa
    16321 acactccagc gcctgcgtga tgctaaaccc cagggtggca ttggtctgct gacggtgaaa
    16381 ctggatgatc cgaccggtta tggacgtatc acccgtgaaa atggcaaagt taccggcatt
    16441 gttgagcaca aagatgccac cgacgagcag cgtcagattc aggagatcaa caccggcatt
    16501 ctgattgcca acggcgcaga tatgaaacgc tggctggcga agctgaccaa caataatgct
    16561 cagggcgaat actacatcac cgacattatt gcgctggcgt atcaggaagg gcgtgaaatc
    16621 gtcgccgttc atccgcaacg tttaagcgaa gtagaaggtg tgaataaccg cctgcaactc
    16681 tcccgtctgg agcgcgttta ccagtccgaa caggctgaaa aactgctgtt agcaggcgtt
    16741 atgctgcgcg atccggcgcg ttttgatctg cgcggtacgc ttactcacgg gcgcgatgtt
    16801 gaaattgata ctaacgttat catcgagggc aacgtgactc tcggtcatcg cgtgaaaatc
    16861 ggcaccggtt gcgtgattaa aaacagcgtg attggcgatg attgcgaaat cagtccgtat
    16921 accgttgtgg aagacgcgaa tctggcagcg gcctgtacta ttggcccgtt tgcccgtctg
    16981 cgtcctggtg ctgagttgct ggaaggtgca cacgttggta acttcgttga gatgaaaaaa
    17041 gcgcgtctgg gtaaaggcac gaaagccggg catctgactt acctgggcga tgcggaaatt
    17101 ggcgataacg ttaacatcgg cgcgggaacc attacctgca actacgatgg tgcgaataaa
    17161 tttaagacca ttatcggcga cgatgtgttt gtcggttccg acactcagct ggtggccccg
    17221 gtaacagtag gcaaaggcgc gaccattgct gcgggtacaa ctgtgacgcg taatgtcggc
    17281 gaaaatgcat tagctatcag ccgtgtgccg cagactcaga aagaaggctg gcgtcgtccg
    17341 gtaaagaaaa agtaattctg gccgggtaat ccggtcatct gggatgagga gataacataa
    17401 tctccctccc acaagcagta actataaaaa taaccccact ctctacaagg ctcggggcgc
    17461 ccgaaaaaac gggcatacag gttgaccgac aacgatataa atcggaatca aaaactatgt
    17521 gtggaattgt tggcgcgatc gcgcaacgtg atgtagcaga aatccttctt gaaggtttac
    17581 gtcgtctgga ataccgcgga tatgactctg ccggtctggc cgttgttgat gcggaaggtc
    17641 atatgactcg cctgcgtcgc ctcggtaaag tccagatgct ggctcaggca gcggaagaac
    17701 atcctctgca tggcggcacc ggtattgctc acactcgctg ggcgacccac ggtgaacctt
    17761 cagaagctaa cgcgcatccg catgtttctg aacacattgt ggtggtgcat aatggcatca
    17821 tcgaaaacca tgaaccgctg cgtgaagagt taaaagcgcg tggctatacc ttcgtttctg
    17881 aaaccgacac cgaagtgatt gcccatctgg tgaactggga gctgaaacaa ggcgggactc
    17941 tgcgtgaggc tgttctgcgt gctatcccgc agctgcgcgg tgcgtacggt acagtgatca
    18001 tggactcccg tcacccggat accctgctgg cggcacgttc tggtagtccg ctggtgattg
    18061 gcctgggtat gggcgaaaac tttatcgctt ctgaccagct ggcgctgttg ccggtgaccc
    18121 gtcgctttat cttccttgaa gagggcgata ttgcggaaat cactcgccgt tcggtaaaca
    18181 tcttcgataa aactggcgcg gaagtaaaac gtcaggatat cgaatccaat ctgcaatatg
    18241 acgcgggcga taaaggtatt taccgtcact acatgcagaa agagatctac gaacagccga
    18301 acgcgataaa aaacaccctt accgggcgca tcagccacgg tcaggttgat ttaagcgagc
    18361 tgggaccgaa cgccgacgaa ctgctgtcga aggttgagca tattcagatc ctcgcctgtg
    18421 gtacttctta taactccggt atggtttccc gctactggtt tgaatcgctg gcaggtattc
    18481 cgtgcgacgt cgaaatcgcc tctgaattcc gctatcgtaa atctgccgtg cgtcgtaaca
    18541 gcctgatgat caccttgtca cagtctggcg aaaccgcgga taccctggcg ggcctgcgtc
    18601 tgtcgaaaga gctgggttac cttggttcgc tggcaatctg taacgttccg ggttcttctc
    18661 tggtgcgcga atccgatctg gcgctgatga ccaacgcggg tacggaaatc ggcgtggcgt
    18721 ccactaaagc attcaccact cagttaactg tgctgttgat gctggtggcg aagctgtctc
    18781 gcctgaaagg tctggatgcc tccattgaac atgacattgt gcatggtctg caggcgctgc
    18841 cgagccgtat tgagcagatg ctgtctcagg ataaacgcat tgaagctctg gcagaagatt
    18901 tctctgacaa acatcacgcg ctgttcctgg gccgtggcga tcagtaccca atcgcgctgg
    18961 aaggcgcatt gaagctgaaa gagatctctt acattcacgc tgaagcctac gctgcaggcg
    19021 agctgaaaca cggcccgctg gcgctgattg acgcggatat gccggttatc gtcgttgcac
    19081 cgaacaacga actgttggaa aaactgaaat ccaacattga agaagttcgc gcgcgtggcg
    19141 gtcaattgta tgtcttcgcc gatcaggatg cgggttttgt aagtagcgat aacatgcaca
    19201 tcatcgagat gccgcatgtg gaagaggtga ttgcaccgat cttctacacc gttccgctgc
    19261 agctgctggc ttaccatgtc gcgctgatca aaggcaccga cgttgaccag ccgcgtaacc
    19321 tggcaaaatc ggttacggtt gagtaatcac tatcatagat aaaaaagggc tgccg
//
LOCUS       DAEEUE010000078        19262 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.078, whole genome shotgun sequence.
ACCESSION   DAEEUE010000078 DAEEUE010000000
VERSION     DAEEUE010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19262
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.078"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            32..331
                     /locus_tag="KLK84_002886"
     CDS             32..331
                     /locus_tag="KLK84_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HBE2010138.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            328..2076
                     /locus_tag="KLK84_002887"
     CDS             328..2076
                     /locus_tag="KLK84_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBE2010139.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPAEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            2365..2643
                     /locus_tag="KLK84_002888"
     CDS             2365..2643
                     /locus_tag="KLK84_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010140.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            2640..2945
                     /locus_tag="KLK84_002889"
     CDS             2640..2945
                     /locus_tag="KLK84_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010141.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            2955..3116
                     /locus_tag="KLK84_002890"
     CDS             2955..3116
                     /locus_tag="KLK84_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HBE2010142.1"
                     /translation="MTAQIAAYGRLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            3258..3482
                     /locus_tag="KLK84_002891"
     CDS             3258..3482
                     /locus_tag="KLK84_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010143.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            3783..4940
                     /locus_tag="KLK84_002892"
                     /pseudo
     CDS             3783..4940
                     /locus_tag="KLK84_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            4980..5552
                     /locus_tag="KLK84_002893"
     CDS             4980..5552
                     /locus_tag="KLK84_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBE2010144.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            5554..6765
                     /locus_tag="KLK84_002894"
     CDS             5554..6765
                     /locus_tag="KLK84_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBE2010145.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            6762..7100
                     /locus_tag="KLK84_002895"
     CDS             6762..7100
                     /locus_tag="KLK84_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBE2010146.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            7097..7393
                     /locus_tag="KLK84_002896"
     CDS             7097..7393
                     /locus_tag="KLK84_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HBE2010147.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            7393..7833
                     /locus_tag="KLK84_002897"
     CDS             7393..7833
                     /locus_tag="KLK84_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBE2010148.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            7817..7999
                     /locus_tag="KLK84_002898"
     CDS             7817..7999
                     /locus_tag="KLK84_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010149.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            8055..8345
                     /locus_tag="KLK84_002899"
     CDS             8055..8345
                     /locus_tag="KLK84_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HBE2010150.1"
                     /translation="MDDIAVKQWREKSRQLAEQGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPP"
     gene            8365..10026
                     /locus_tag="KLK84_002900"
     CDS             8365..10026
                     /locus_tag="KLK84_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBE2010151.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            10040..10321
                     /locus_tag="KLK84_002901"
                     /pseudo
     CDS             10040..10321
                     /locus_tag="KLK84_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11118..12578)
                     /gene="phoQ"
                     /locus_tag="KLK84_002902"
     CDS             complement(11118..12578)
                     /gene="phoQ"
                     /locus_tag="KLK84_002902"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HBE2010152.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(12578..13249)
                     /gene="phoP"
                     /locus_tag="KLK84_002903"
     CDS             complement(12578..13249)
                     /gene="phoP"
                     /locus_tag="KLK84_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HBE2010153.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(13418..14788)
                     /gene="purB"
                     /locus_tag="KLK84_002904"
     CDS             complement(13418..14788)
                     /gene="purB"
                     /locus_tag="KLK84_002904"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBE2010154.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(14792..15433)
                     /gene="hflD"
                     /locus_tag="KLK84_002905"
     CDS             complement(14792..15433)
                     /gene="hflD"
                     /locus_tag="KLK84_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HBE2010155.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(15469..16575)
                     /gene="mnmA"
                     /locus_tag="KLK84_002906"
     CDS             complement(15469..16575)
                     /gene="mnmA"
                     /locus_tag="KLK84_002906"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBE2010156.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(16629..17090)
                     /gene="nudJ"
                     /locus_tag="KLK84_002907"
     CDS             complement(16629..17090)
                     /gene="nudJ"
                     /locus_tag="KLK84_002907"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HBE2010157.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(17100..17753)
                     /gene="rluE"
                     /locus_tag="KLK84_002908"
     CDS             complement(17100..17753)
                     /gene="rluE"
                     /locus_tag="KLK84_002908"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HBE2010158.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            17925..19175
                     /gene="icd"
                     /locus_tag="KLK84_002909"
     CDS             17925..19175
                     /gene="icd"
                     /locus_tag="KLK84_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HBE2010159.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
BASE COUNT         5016 a         4727 c         5159 g         4360 t
ORIGIN      
        1 ataaagaaca acacaccgga gaataaaaat aatgaaactt aaatattctg gcttaactgc
       61 cagtggcaac actcacccta aatttacgcg cggtgatatt taccgcgacc agtacggcgg
      121 catggtaacg attaaaggcg tggcagaacg gcgcatcacc taccgccgtg aaggttacga
      181 atatgattgc gtgatgcctg tttatcaatt ccggcgtgat ttcacgctgg tacaggcagc
      241 gccccgcagt aaaccaacca gcagggagaa agcacgcgcc aatattcagg aaataaaaaa
      301 gatgcttaac gtattcaggg gtaaaaaatg aaactggcac cgaacgtaaa aaaacagcca
      361 cgcggaataa aacacaaaga cacagaggta attatctttg cgggtagtga tgcctggtcg
      421 cacgcgaaac aatggcagga gcaggatggc cccgcatccg gcgataatgt gccgcctgtg
      481 tggcttgggc caaatcagct tgccgaactt gatgcactga aaattgttcc ggatggaaaa
      541 aaacgcgtaa ggctgtacca ggccggagaa ctggatttgg tggagaccaa aaagattggt
      601 cagaagctgg cggcggcaga tattcaggac gcaaattttt accccgaagg aatgcacgtc
      661 cagaagtgtg aaaactggcg gcgctatctg aatgctgagc gtgaaaatat tgccgcaggg
      721 cttaccatgc cggagcagaa aaatacgcaa ctggcacaaa tggcagacag tgagcgcgcg
      781 cagctgcttg ccgagcgctt tgatggcgtt tgtgtgcatc aggaaagtga aatcgttcac
      841 gtatggcgcg gcggggtatg gtgtccggtc agcacaatgg aacttagccg cgaaatggtg
      901 gcgatctatt cagagcacag ggccactttc agcaagcgcg taatcaataa cgccgtggaa
      961 gcgttaaaag ttattgccga acccatgggc gagccgtccg gcgatttgct gccgttcgcc
     1021 aatggtgcgc ttgacctgaa aacgggggaa ttttccccgc acacgccgga gaactggatc
     1081 accacgcaca acggcattga gtacacgcca ccagcaccag gggagaacat ccgcgataac
     1141 gcgctaaact ttcataaatg gcttgagcac gcagccggaa aagaccagcg caagatgatg
     1201 cgtatatgtg ccgcgctgta catgattatg gcgaaccggt acgactggca gatgtttatt
     1261 gaggccaccg gagacggcgg gagcggtaaa agtacattca cacacatagc cagccttctg
     1321 gcagggaaac agaacacggt aagcgctgaa atgacatcgc ttgatgatgc tggtggacgt
     1381 gcgcaggttg tcgggagtcg tcttatcgtc ctggctgacc agccgaaata tacaggcgaa
     1441 ggaacgggca tcaagaaaat cacgggcggt gaccccgcgg aaattaaccc gaaatacgaa
     1501 aagcgtttca cggctgtaat cagggcggtg gtgctggcga ccaacaataa cccgatgata
     1561 ttcaccgaac gggccggagg tgtggcacgt cgtcgcgtga ttttccgttt cgacaatatc
     1621 gttagtgagg ccgaaaaaga cagggagcta ccggaaaaga ttgcggctga aatccctgtc
     1681 attatccgcc gattgctggc gaactttacc gactctgaga aagcacgggc tttactactg
     1741 gaacagcgtg acggtgatga agctctggca ataaagcaac agacggatcc ggttattgag
     1801 ttttgccagt tcctgaattt tctggaggaa gcacgcggcc tgatgatggg cggcggtggc
     1861 gattcagtga agtacacgac cagaaacagc ctttaccgcg tctatctggc gtttatggcg
     1921 tacgcaggca ggagcaaacc gctaaacgta aatgactttg gcaaggctat gaagccagcc
     1981 gcgaaagttt acggacatga atatattacg cggaaagtta agggagtaac gcagactaac
     2041 gcaataacaa cagacgagtg cgacgcgttt ttataatttt ttgtaattgc tgtctaccct
     2101 gtctacctga gtaaagaaaa atacatttaa ttcagtgcat taagttaggt agatagcctt
     2161 tttttactgt ctacccacta tctaccctct ctacctgatt ttacctgaat cagacaggga
     2221 ggtagacacg gggtagatag tggataaaag cactctaccc cgctgaaagc agcgccatta
     2281 ctggcatgag ggccactaag gtagataagg tagacaaggg gaggcacaac tcaaaacttt
     2341 ttaaacgagg gggtaaaaat aaatatgcac acttcaggga aatttaataa atcattcaaa
     2401 aaacgcagag acagaacaga accgaaatat cgcgcgttag acatgacaga gcacgcttta
     2461 aaggtggcaa tcagaacgat agaccgccac gcgggggaag gatacgcgaa agcacatccc
     2521 gaactgataa gcgcattcat gaccacgacg gcggcaaatt ttgccacgct gacagagcgg
     2581 gagattgccg aagcggaaca ggtaacaaca atcaacgtta aaaccggaga ggtggaatca
     2641 tgagcgaacc aaaatttggt gaaaaatttt acaagcacaa cggacggatc acgattctgc
     2701 aaataagcgc agctacacca ggctggtggg ttgaaacaga cgaaggatcc tcacccgtgg
     2761 catcatgggc gctttgtgtg cttagttacc ctgaccgcga tgtgtatcag gatattctgc
     2821 ctgtgatatc cactgacaaa ggcatgaaac ccgtagacat caaaaacatg gggtttcagt
     2881 gcgtaatgct cactgaaaaa atgatggaag agatgaaaaa gaacaatgcc ggatcgcttc
     2941 actgaggtgg caatatgaca gcacaaattg cagcttacgg gcggctggtg ggcgacccgc
     3001 tggtaaaaca gagcagcaag ggcacaccgg agccgccaac ggagaactac gcagacctga
     3061 gaaatgcagt aaatcgcact tacgcagaca tggggctgag caaattagca gagtaggaat
     3121 atcagaacca caccacgcag ccggagcaat ccggcttttt ttgcgtcatt tgaaacggta
     3181 tggagcacta ctggcttttc gtcacggtcc ggcatagtta ctatctgaaa caaacagaca
     3241 caacagagga aaacgcaatg ccgatgaaat ttgatgagat acttaaacag cgtgataaat
     3301 accatgctga caacatggag acgatgaata tcactgatta ccgcgcattt ctggaaacgg
     3361 gggcgctgat tgaaaaggat catcatggtt ttgttcgttg cgcgctatcc ggtgaaatgc
     3421 tggcagtaaa tcctgaacag acagatgcat tgatagaatt tctgaaaggg atcagagact
     3481 gagcacccat acagcacata cagccggagc aatccggctt tttgttattt tttgtaaatt
     3541 atttgttcgt ggttgttcca cgttgttcac tgagcggatc ggcatatttt acccgaactg
     3601 aatcatgatt attctcgccc gtggtgccag gacgctgggg ccactttccc gcctgttaat
     3661 gtgctcgcca atattcatta ccaggcggga aaacgatcgg tgcgattact gatttcctta
     3721 tgaaaaacgg ttgagtgttt gccgcgtcct ggagttcctt acttaacccc aggacttttt
     3781 ttatgcagag aataatcgaa ttacgccagc agaaaaccgc cattaaaaat cagatgcgcg
     3841 acatgctgga gaacgctgaa aaagaaaacc gcagtcttaa cgatgctgag ggcacaaaat
     3901 ttgacgaatt acgcgctaaa gctgaatccc tcgataaaga catttcccgc cttgaagcca
     3961 ttgcagacga agagcgcagc aagccaggta aaagcagcca gaccactgac cccgcagaac
     4021 tacgcaacta cattctgacc ggtgaaaccc gcgcattaag tacaggcgtt cccgctgatg
     4081 gtggttatac cgttatccct gaactgaaca ccgaaatcat gcgaatgctg gcggatgaat
     4141 ccaccatgcg tcgcatctgt accgtgaaga aaatcagcag caacgagttt aagcagcttg
     4201 tttccgctgg cggtgcgacc gttaaccacg gtgaagaggg taaggcacgc gaacagacca
     4261 gcaccccgca gattaacgag gtgagcatta agctgtatcc ggtctatgcg tacccgcgca
     4321 ccacacagga aatcgtggat ttttccgatg tggacatcct ttcatggctg acgggtgaga
     4381 ttggcgacac cttcacggaa accgaagaaa gcgatctggt tgtgggcgac ggtgacaaaa
     4441 aagcaaaagg ttttttatcc gtaccccgtg cagagaagaa cgacaaagag cgtgattttg
     4501 gtacgttgca ggtaattaaa ccttccgaat ctctggcgtg gacatctgcg gacccgctga
     4561 tcgacctgaa atttgcatta cgtaaaaaat accgcaaaaa cgcggtctga gtggttaact
     4621 ccacgacggc ggcaaaactt cagaaggtga agaacgcgaa cggtgattat atctggcgcg
     4681 accgtttaca gacgggtgat cctgatacgt tgctgggcct tccggtcgaa tatctggagt
     4741 ttatgcctga taacgttatt gccctgggtg acttcaaacg cggttactac attgttgatc
     4801 acgaaacagg tgttcgcacc agaccggaca acctgacaga accaggattc atcaaaattt
     4861 tcacgcaaaa atatttaggc ggtggcgtgg tggattcgaa cgcgatcaag attctggcac
     4921 tgccggaaga cgacgattaa cagcatacag aaggggctaa aaagcccctt tagtgtttta
     4981 tgggtgaaaa aattatgaag agtatggaaa tccggtcatc ggaaatcacc accagcggag
     5041 ccggtacgct gacgggctac gttgttcgct gggataagct ttcagaactg ttatggggtg
     5101 agttttacga aaaattccag cggggggcgt ttactgaatg gcttgcggcg ggtaatgacg
     5161 ttcgcggcct gtatgagcat gaccacagca tgttactggg gcgcacccgt tccggcacgc
     5221 tgaaactgga agaggacgaa acagggttac gctttgaact gaccccaccg gataccagta
     5281 cagggcgcga cgttatcgaa ctggttaagc gcggggacat ctcggggatg agctttggct
     5341 ttcgctcccg taaggatgta tgggatacca caacagatcc atgcgtgcgc accgtgctgg
     5401 tggcggaact gtacgaaatt accgttacat cggtaccggc ttaccccgat tccggcgtgg
     5461 agctggcccg ccgttccctg tatgagcagc accccgaaaa aatgccgcgt gcggataatc
     5521 gccgctggtg ggcggattta gcgggggtgt gatatgtggc ctttcagacg aaaaaaagag
     5581 cagcgcagca tgacgcttga tgaatttatg gcgctggccg gcacatcgaa cacgggggcg
     5641 ggtgagtacg tatcatctgg gacagcggaa tcactccccg ccgtcatgaa cgccgtcacg
     5701 gtcatctctg acgcggtggc taccatgccg tgttacctgt acctggtacg caatgagaag
     5761 gggaaggagg cccgcgaatg gcttgattct catccggtcg atcacatcct caacgagcgc
     5821 ccgaacgcgt ggcagacacc ctaccagttt aagcgaatga tgatccgcta ctgcctgtta
     5881 agtggtaatg cttatgcggt tattcagtgg gggcgtgatg gttttccggc ggctttacat
     5941 ccttacccgc cgcagtcggt gaacgtggag cagacaggtg atcataactg gcgctactgc
     6001 atcactgacg cctacaccgg aaacacccgc aactatttac cgtgggaggt tctccacctt
     6061 cgttactcca cggatgacgg ttttatgggg cgctcacctg taaccatctg ccgcgaatcg
     6121 ctggggcttg ggctggccca acaacgccac ggcgcgagcg tgatgcgtga tggcatgatg
     6181 gcggcagggg ttatcacgtc aggcgaatgg ctggacggcg tgaagggcaa acaggcatta
     6241 gccgcactgg aacgctacaa aggggccaga aacgccggaa aaacgcccat ccttgaaggg
     6301 gggatgagct atcagcagct gggcatgagt aatcaggatg ccgaatggct ggcctcccgt
     6361 cgcttcacca ttgaagacat cgcccgaatg ttcaacgtct cgccgatttt tttgcaggaa
     6421 tacagcaaca gcacctacag caacttcagc gaggcaagcc gcgcatttct caccatgacg
     6481 atgcgcccgt ggctggcgaa ctttgagcag cagataaaaa acgccttgct ggtggcctcg
     6541 cctgtacctg gtatccggta tcaggtggag tttgacagcg cggacctgtt acgggccaca
     6601 cctggcgaac gctttgccac ctatgagcgc ggcataaaat ccgacgttat gtgtccgaac
     6661 gaagcccgcg aacgtgaagg gctgtcaccg cgtgatggtg gtgatgagtt cagccaggca
     6721 tggaaacagg aagtaaaaat cagcgaggga gaaaaaccgg aatgaacata gggcgactgc
     6781 gtgacaggat aacgattcag accctgaaac agaccaggga tataaccggc gaaatactcg
     6841 aaacgtggga ggacggtcac acactctggg caagcgtgaa catggtcagc agcaaggagg
     6901 ccatttcatc gggcgcagag ctggcgattg gtaccgtaag gatctggata cggtaccgga
     6961 aggacatcaa cgccaccagc cggataaagg tcagtacggg gccgctggcg gggcgtgtac
     7021 tgaatatcat cgggcagccg ctgccggatg ccgccaggac acgccttgaa attctttgtc
     7081 gtgagggcgc ggaaaaatga cagaagaact tatcaccctg gaagaagtga aactccattg
     7141 ccgcatcgat ggcgacgatg aagaccagtt aatcagcgga tacattgccg catcgcttga
     7201 ggcgtgccag atacacatag gcaggcgctt tgatgacggg ctggcgttca cgccagccat
     7261 aaagattggc tgcatgatgt ttatcgctca cctgtatgaa aatcgccaga ttgtcgcgga
     7321 taacgcaaaa acacgcgtac ccatgacgat aggcgcactc tggacggctt accgtgatgt
     7381 gggggggtac tgatgccgtg gcaaccatta agacgatgca cagaaccagg ctgtaacagg
     7441 cgcgttaaat ccggcaagtg tgaggagcac aggcgggcgg catggcgtgc agaggatgcc
     7501 agacgaggac accgccgcgc gcgtggatat tccagacagt gggacaaata ccgcgccatg
     7561 tatctgagta aaaacccgtt atgcgtgcga tgccttgaga aggggatata tacgcccgcc
     7621 gtggtggtgg atcacattat cccgattaat ggcggtgatg atgttctctt ctggcccgaa
     7681 tggaaccatc aaccattgtg ccaggcgtgt cataaccaga aaacgaaatg gcttgatccg
     7741 gcaacaaaaa gcaagcgtgc cgcaggtgga tttcgtgaag aggaagaacg ggctgctaac
     7801 cgcaataact ggatgtatga cgctgatgaa tgagcgggaa caaaaccgcc ttatccgtgg
     7861 acttataagg cagcgtgacg catggaagac acaggaaaca gggcataaag ataaagcgtc
     7921 aggacgcgca gaacgcatca cagcgaagcg attaaccgac cgtgaccgcg aggttatgga
     7981 atgtttccgc aatcgctgat gaggctgtat gacggggtgg ggggcgtttt caggacaaac
     8041 ccgaccccgc ctacctggat gatatcgccg tgaagcagtg gcgggaaaaa tcgcggcagc
     8101 ttgcggaaca gggagacctg acccccgccg actggagcaa tctggaactg tattgcgtca
     8161 actactccat ttaccggaaa gccgttgcag accttgcggc gcgcgggttc agcattgtta
     8221 acagtcaggg cggcgagagc agaaaccccg cattaagcgc aaaatccgac gctgaaagag
     8281 tgatgataaa aatggcctcc ttgctcggtt ttgacccgat aagccgccgt aaaaatccac
     8341 cgtaaacaga agaagaggac gagcttgacc gcctggaata agtacgcaga agacgtaaaa
     8401 acgggcaaaa ttccggcctg taaacggtta aaacaggctg ttaaacggta cttttcggac
     8461 cttaaaagcc ccctttacac gttcgatcgt gaggttgtgg agcggtttat tgccttttcc
     8521 agggtgtgcc cgcacgtaaa aggaccgatg cgtggcagac ccattgagct ggagccgtgg
     8581 cagcagttcg cctttgcgtg catcctcggc tttaaggtta aggccaccgg acggcgcaaa
     8641 tacacgagcg cctttatcga agtgccgcgc aaaaatgcga aatccacgac cgccgcgatt
     8701 ctggctaact ggtttctgat tatggagaac ggtcagcagg atatctacac cgcagcggtg
     8761 agccgtgacc aggcgcggat cgtgtttgat aatgcgcgtc agatgtgcct tttatcccga
     8821 ccgttacgca ggcgggtgaa tattcaggcg cataaggtga tacacccgaa aagcaacagc
     8881 ctgttaaagc cgctggcagc aaaagcggca accatcgagg ggacaaaccc cagtctttcc
     8941 atcgtggacg aataccacct acaccccgat aacggcgttt attccgcgct tgagctgggg
     9001 atgggcgcac gtcccgaagg gctgttattt gccattacca catcgggcag taacgtcgtt
     9061 tcagcctgta aacagcacta tgattattgc tgccagatcc tggacggcga agaggtcaac
     9121 gattcaattt ttgtactgat ttacgaactg gacgacgaaa acgaggttga tgatccggcg
     9181 atgtggataa aggcgaaccc taatattgat gtttccgtgg atcgtgaaaa actggcctca
     9241 accatccaga aagcgcgggg tattccgtcg cagtgggtgg agatgatgac aaagcgattt
     9301 aatatctggt gtcaggggac taccccgtgg atgggtaatg gtgcatgggc tgagtgtgcc
     9361 ggaacgttca cggaggaaga tttacacggt caggagtgtt acgcggggct ggacttatca
     9421 tcaaccagcg atatttccag cgtatgctat gcctttccgg tcggtaaaac cattatgctg
     9481 atttcccgtc attatctgcc ggagttccag ctacagaacc ccgccaataa aaaccgcgct
     9541 gtctatcgcc agtgggctaa agcgggctgg atacgcacaa cgccaggtga ctgcattgat
     9601 tacgaccgga tcagagatga catcatgcag gatgcggaga aatttaatat caggctggtg
     9661 ggctttgata cgtggaacgc cacgcatctc aggacgcaat tacagggggc tggttttgag
     9721 gtggagccgt tcccgcaaac ctacctcaga ttcagtccgg cggcgaaatc gttcgaagtt
     9781 tttgttaacc gcagggtgat tgtgcatcgt ggcgatccgg tgttgtcctg gtcgatgagt
     9841 aacgtcgtga tgcagagtga cgcgaacgcc aatatcaagc cgaacaagaa aaaatcaccg
     9901 aacaagatag acccgagcgt agcggcgctg atggcgtttg gtacattcca ggcagagcat
     9961 gaggattttg cttttgatat aagcgacagc caccgccaga aactggaaga atttagcggg
    10021 gtgtaatgag gtcagcagca tgacagaagc cgaaatactg cgattaatcc gccgcgtctc
    10081 tggaatcagc cagtaggctg acgaacagac cacgcagccg gacagcgtga cagccgaaaa
    10141 ttatgcgcgt gttgttgctg aggtgatgcg ccgtgatggt atccagctta atgatgcgga
    10201 tatgcgcgac atacggatcc gcgttctgga aatgctggcc tacaatcgcc gcgttgaact
    10261 gtatcgggag aaggagaaaa tcacgtacca ctggaagaag ccggagcggt tacggcggta
    10321 actcactgag attaaagcaa agcgcaaaat tgcgctggct acatccctca catctgagga
    10381 ctgttaacca gcaggccgga atatattcag aaattacgaa attttcgcag tttaattact
    10441 cgtcagctta atcgctaacc cgctctgata ttttcggaaa cctcaatttg aggaagtcgg
    10501 cgaggtaact gtttgatatt cccgatagcg caaaaatgcg cggtcgggca gaatgaccac
    10561 cacctcaatc gtgacggaga gtgacaacca gtgacacacc gtgacaaaaa gacggtttaa
    10621 ccgttcatta aaaatcgacg tgattttgtg ctttccggat gacgtggcca acatcatttt
    10681 ttagcgaaaa attctgatta aggtcactgg ctgaacggtc ataatgacca cgcagaccat
    10741 cgaggcaacc atagcgatca tcgttacggt tggagctttt ccgccattgg tggaaaaggt
    10801 gcacatctgg atcatcatta cggtgatcaa gggtatgtaa aacccaccag cctgattaaa
    10861 ggttaaccgg aaaaaaaatc cggtagcccc gttatgggga tccccatatc gacattaacg
    10921 cccctcatga attggtgcgc ttccccctgg aaagattacc cgcctgtata tttcttgtgt
    10981 ctatttgttc cacgttgttt catacagtgc accgaacggt gtagttaatg gtgtagtcaa
    11041 tttgcgattt ttggcacttt ttcaatgaaa attgtttgat cgaatcaatg acttgatgta
    11101 gtggtaaaag gacatattta ttcatctttc ggcgcagaat gctggcgacc aaaaatcacc
    11161 tccatccgcg caccgcccag catgctctct ccggcgacga ttttaccctc atattgctcg
    11221 gtgatttcgc gggctaccgc cagccctaca ccttgcccag ggcgtaaagt atcaacccgt
    11281 tgaccacggt cgaaaatgac ctctcgcttg cttaatggaa taccggggcc atcatcctcg
    11341 accacaatat agagatgctc gtcggtttgc cttgcagaaa tttcgacaaa ctcgaggcaa
    11401 tatttacagg cattatccag cacgttgccc atcacctcga caaaatcgtt ctgctcaccg
    11461 acaaagctga tctctggcga aatatcgaga gagatattga cccctttgcg ttgatacact
    11521 ttgttcagcg ctgaggtgag attgtccagc agtggggcga ccggatgcag ctcgcggctg
    11581 agcaatgtcc cgccgcgcat actggcacga tgcaggtagt agccaatttg ctgtgaaatg
    11641 cggctgattt gctccagcat taccggctca gcatcactga cgctcatctt ttcactacgc
    11701 agagaacgca gcgtactttg cagcaccgcc agtggcgttt tcagactatg ggtcaggtcg
    11761 gtgagcgtcg tacggtattt gtcgtaacgt tcgcgttcac tttttaacaa tcggttcagg
    11821 tttcgtacca gactggtcag ttctcgcgtt gtggctggat tgagcaattc gcggttatgt
    11881 tcttccagtt cgcggacttc ttttgccagg gcttcgatgg ggcgtaaact ccaccaggcg
    11941 gcgacccaca gcagcgggat cactaacagc agattggctg agagcacata gataaaccag
    12001 ctccagacca tataggaact ttttagctcc accggaatgg tatccaccac cacaatggtt
    12061 aattttggca tccgcgatgt tgccgggtag acgtttaccg ccaccgagtg ggtcatctcc
    12121 gcgtcgtcat catcttcccg cacttcctgc aactgttgct gtatcgaatg atctccactc
    12181 agcaagaggc tggtatcgtt aacatccgct tcaatttcat gaaaaccatt cgatttcagc
    12241 cagtcaggct ggatcatttt catcagccag ggcacgtcac gttgcgccca taaaagctgc
    12301 ccgttctcat cataaattag cgtcatggtg gggctttgct tgtcgatatt ttcgggtaac
    12361 tcgacatgca acttattgtt ttcccactgc gcaagggtat agaacagatt gctctcgcca
    12421 cgtaacagcc gaaacgtagt tttatcgaaa ctgacgctat aaccgatcag cgcgaccatt
    12481 ccgtaggcaa gcgaaagcac cagtactacc gctgccgttg ccaacagaaa acgtacccgc
    12541 agcgagagcg ggaaaaaaag acgcagtaat tttttcatca gcgcaattcg aacaggtagc
    12601 cctggccgcg aacggtggta atgacttctt gcggatattg tgcctgaatt tttttgcgca
    12661 gacgtcccat cagtacatca atggtatggc tttcccgcag ctccgcatcc ggatagagtt
    12721 gtagcattaa cgaatctttg ctgaccactt tgccattatt gcgtatcaac gtttccataa
    12781 tggtgtattc gaacgcggtc agtttgatca cttcgtcatt aatagataat tcacggcgag
    12841 agagatcaac ctgaaacggg gggagcgaaa tgacctgtga agccagaccg ctattacgcc
    12901 gcattaatgc ctgcattcgc gccatcacct cttcaatatg aaacggttta gtcacgtaat
    12961 catcagcacc ggcacttaat acttcgactt tgtcctgcca gctttcacgg gcggttaata
    13021 ccagaatcgg cagtgaaaca tcgttgctac gccagcggcg aatcagtgac agaccgtcct
    13081 cgtcaggcaa tccgagatcg acaatcgcaa tatccggtaa atgttcattg agataataat
    13141 cggcttcttt ggcatcttct gcgtcatcga cctgatgacc agcatcctga atctgaactt
    13201 taaggtggtg acgtaacaac gcattgtctt caacaaccag tacgcgcatt tttatttctc
    13261 cctgtcttaa ttattaaaat agtgtaacgc gattatgtgt ttatgggggt aaacattaaa
    13321 taaaccagcg gggaggggag gtaaagtgaa aaaagaaaaa gtggataatc ttaataagca
    13381 ggccggacag catcgccatc cggcactgat ttgaggctta tttcagctca tcaaccatcg
    13441 tgatagctcg accaatatag ttagccggcg tcatcgcttt caggcgggct ttctcttctt
    13501 ctggcaacgc cagaccatcg ataaactgct tcatgccttc ggcgtcaacg cgcttaccgc
    13561 gagtaagctc tttcagcttc tcgtacggtt tttcgatgcc atagcgacgc ataactgtct
    13621 ggattggttc tgccagcact tcccagttgt gatccagctc atccagcaga tggtcacggt
    13681 tcacttccag tttgctcacg cctttcaggg tggattgata cgcaatcagc gcataaccga
    13741 tacccacgcc gaggttacgc agcacggtgg agtcggtcag gtcacgctgc cagcgggaaa
    13801 ccggcagttt gcttgccaga tgctgcaata ccgcgttgga aaggcccaga ttcccttcgg
    13861 agttttcgaa gtcgatcggg ttaactttat gcggcatggt ggaagaacca atctcaccag
    13921 caatggtttt ctgtttgaag tggttaaggg cgatataacc ccagacgtca cggtcaaagt
    13981 cgatcagaat ggtgttgaag cgcgcaacgc aatcaaacag ttcggcaatg tagtcgtgcg
    14041 gttcaatctg ggtagtgtac gggttccatt gaatacccag cgaggtgacg aactcttcgc
    14101 tgaactgatg ccagtcaact tccgggtaag cggcgatgtg ggcgttatag ttaccgaccg
    14161 cgccgttgat tttgccgagg atctccacct ggttaagctg gcggtgctgg cgctccatac
    14221 ggtaggcgac gtttgccatc tctttaccga tggttgacgg cgtggctggc tgaccgtggg
    14281 tacgagacag cagcgggata tcgcggtact gaacggcgag atctttaatg ccatcaatca
    14341 gttgacgcca gtacggcagg atcacttcat cacgcgcggt tttcagcatt aatgcgtggg
    14401 agaggttatt gatatcttcc gaagtacagg caaagtggat gaattcagaa accgcgtgca
    14461 gttccgggat ctccgccact ttttctttca ggaaatactc aaccgcttta acgtcgtggt
    14521 tagtggtacg ctcgatggtt ttgatgcgtg cggcatcttc ttcgctgaaa ttggcgacaa
    14581 ttgcatcaag gtaaccgatt gcgtcggcag caaaagcagg aacttccttg atcgctgcgt
    14641 gcgcggccag tttttgcagc caacgtactt caacttgtac acggaatttc agcaaaccat
    14701 attcgctgaa aatcccgcgc agcgcgctga ctttatcgcc gtagcgtcca tcgacagggg
    14761 aaacggcggt cagtgaggat aattccatag atcacaactc cggggttaaa tgagcaagaa
    14821 tttgttttgc ctgagtggtc aggcgattac gagaaaacat cagttgtaga cgtccgccgc
    14881 cgacctggtg ccagagcacg gcggcgcgaa tgcctgccag cagggttgcg cgaactttcg
    14941 cctgcacttg tgggctttgc agtacagcag gggaaccggt gacctgaatg cgcgggccaa
    15001 gcgggctgat cacatcaaca tagatagcag ccatcgcgct catcagcgtt tcggactgta
    15061 aatcgaagtg ttcgagctgg cgttgcaggc cgttgatccg gttgcccaga gtgtcgagcg
    15121 cgcctttcgc tgaggagagt ttgcgctcaa gcaccatcaa gctgagtgtg tagcgggtta
    15181 attcggcgtt taagccctgg caactgctgg cattgagcac gccgagcagg gtttccagcc
    15241 cgacgcgcag gttggcttcg ctaccgccaa aaaccgccag cgtcgagctg gggttcatat
    15301 caataatact gttgagtgag acgtgtagcg catcggcatc acaatgcccc tggtgagcga
    15361 gttgttgcac caggcgtgcc gactgacaaa taccggccag ggcgagggtg atgtcatagt
    15421 aattctttgc cacgttcact gcttccttgt tttaagtaaa gataataatc agaccggcag
    15481 cggcagacgc tgctcaataa taccgccacc gaggcacact tcaccgttat agaagacggc
    15541 agactggccc ggcgtcacgg cggcaaccgg ctcatcgaaa atcactttaa tgcgatcgtc
    15601 gtccagcgcc ttgacggtgc acggaatgtc ggtctggcga tagcgggttt ttaccgtgca
    15661 acgcatagtg ccggtgaatg gttcgcgatc gacccagtgc agctgctggg caatcaaccc
    15721 gacagacatc agccgcgggt gttcatggcc ctgagcgaca accagaatgt tgttttcgac
    15781 gtctttgtcc accacatacc acggttcttc ggtgccttct ttggtgccac cgatacccag
    15841 acctttacgc tgaccgagag tgtgatacat cagcccctgg tgctcaccaa tttcatcgcc
    15901 atcgacggta atgattttgc ccggttgcgc cgggagataa cggcccagga actcgcggaa
    15961 tttacgttcg ccaatgaagc aaatgccggt agagtctttt ttcttcgcgg tgaccagacc
    16021 aagatcttca gcaatcttac gcacctgcgg tttttccagt tcgcctaccg ggaacaggct
    16081 ttgcgcaatc tgctcatggc tgagcgtata aaggaagtag ctctggtctt tattgctgtc
    16141 cagaccacgc agcaggcggc tcttgccatc gacatcggcc cgacgtacgt aatgaccggt
    16201 agcgatataa tcggcaccta aatcttcggc ggcaaattcg aggaaggctt taaatttgat
    16261 ctccttgttg cacagaatat ccggattcgg cgtgcgaccg gctttatatt cggcaaggaa
    16321 cagttcgaag acgttgtccc agtactcggc agcaaagtta acggtgtgca gttcaatgcc
    16381 gagcttgtcg cagaccgcct gggcatcagc cagatccgct gccgctgtgc aatattcctc
    16441 accgtcgtct tcttcccagt tcttcataaa caggccttcg acctgatatc cctgttgttg
    16501 caacagccag gcagaaacgg aggaatcgac accgccggac atgccgacga ttactttttt
    16561 tgcggtttca gacattggat cactcacgac attgaacttc aaggcggcgt attctatcac
    16621 gcagcctttt agatgacacc ctttgtaaaa ggccagttaa aatcgccaat catctccagc
    16681 ggataacgtt gcccgctttg ataacaacga atactttccg ccaccagcgg cgaacgaaga
    16741 tttgacgcct gtaaaatttc ttcggcgctg acccaacggc agcagtcgat atcgctgtca
    16801 tgaggctggg tcgggcatat ttgctcaagc tcaatggcaa agaggaaacg caaaaacggc
    16861 gttttatctg gcgcaatcca ctgatgcata cgaataaagt gttgcggctg cgcgctgatg
    16921 ccggtttctt cccacagttc acgggcggcg gcttccacta aggtttcatc ggcttccaga
    16981 tgcccggcag gttggttcca taacgcttta ccattaatcg tctcttcaac gactaaaaat
    17041 ttgccttctg cgtgcaccac gcaagcaacg gtaacgtgcg gtttaaacat tttgcatcct
    17101 taatctgtca cttctcgcca ttcgccattg gcaagattat ccaaagagta atctcccatc
    17161 gcatagcgaa tcagtcgcag cgtggggaaa ccaacatggg cggtcatgcg gcgcacctgg
    17221 cgattacgtc cttcatataa ggtgatcttc agccagctgg tgggaatact tttgcgttca
    17281 cgaattggtg gattccgcgg ccataaccac gcgggttcgt caaccagctc cgcgcctgcg
    17341 ggcagggtag ggccatcatt taaggttacg ccattgcgca aggcttcaag tgcgtcttgt
    17401 gtgggaatac cttccacctg cacataatag atttttccgg tgcgtttacc cggctgggtt
    17461 aaacgcgcct gcaacgcgcc gttattggtc agcaccagca acccttcgct atcgcggtca
    17521 aggcgacctg ctgcataaac gccctgaacc gggatgaatt cttttaatgt tttgcgtccg
    17581 gtttcatcgg tgaactgcgg aagaacatcg tagggtttat tgaagaggat cacacgcgtg
    17641 ggctggtttt catgtttacg cctggtagaa cgttgtgagc tgaatcgctt aacctggtga
    17701 tttctaaaag aagttttttg catggtattt tcagagatta tgaattgccg cattatagcc
    17761 taataacgcg catctttcat gacggcaaac aatagggtag tattgacaag tcaattacaa
    17821 atcattaaca aaaaattgct ctaaagcatc cgtatcgcag gacgcaaacg catatgcaac
    17881 gtggtggcag acgagcaaac cagtagcgct cgaaggagag gtgaatggaa agtaaagtag
    17941 ttgttccggc acaaggcaag aagatcaccc tgcaaaacgg caaactcaac gttcctgaaa
    18001 atccgattat cccttacatt gaaggtgatg gaatcggtgt agatgtaacc ccagccatgc
    18061 tgaaagtggt cgacgctgca gtcgagaaag cctataaagg cgagcgtaaa atctcctgga
    18121 tggaaattta caccggtgaa aaatccacac aggtttatgg tcaggatgtc tggctgcctg
    18181 ctgaaaccct tgatctgatt cgtgaatatc gcgttgccat taaaggcccg ctgaccactc
    18241 cggttggtgg cggtattcgt tctctcaacg ttgccctgcg tcaggaactg gatctctaca
    18301 tctgcctgcg tccggtacgt tactatcagg gcactccaag cccggttaaa caccctgaac
    18361 tgaccgatat ggttatcttc cgtgaaaact cggaagacat ttatgcgggt atcgaatgga
    18421 aagctgactc tgccgacgcc gagaaagtga ttaaattcct gcgtgaagag atgggcgtga
    18481 agaaaattcg cttcccggaa cattgcggta tcggtattaa gccgtgttct gaagaaggca
    18541 ccaaacgtct ggttcgtgca gcgatcgaat acgcaattgc taacgatcgt gactctgtga
    18601 ccctggtgca caaaggcaac atcatgaagt tcaccgaagg cgcgtttaaa gactggggct
    18661 accagctggc gcgtgaagaa tttggcggtg aactgatcga cggcggcccg tggctgaaag
    18721 ttaaaaaccc gaataccggc aaagagatcg tcattaaaga cgtgattgct gatgcattcc
    18781 tgcaacagat cctgctgcgt ccggctgaat atgatgttat cgcctgtatg aacctgaacg
    18841 gtgactacat ttctgacgct ctggcagcgc aggttggcgg tatcggtatc gcccctggag
    18901 caaacatcgg tgacgaatgc gccctgtttg aagccaccca cggtactgcg ccgaaatacg
    18961 ccggtcagga caaagtaaac cctggctcta ttattctctc cgctgagatg atgttacgcc
    19021 atatgggctg gactgaagcc gcagacctga ttgttaaagg tatggaaggc gcgatcaacg
    19081 ccaagaccgt aacctatgac ttcgagcgtc tgatggaagg cgctaaactg ctgaaatgtt
    19141 cagagtttgg tgatgcgatc atcaagaata tgtaatcact acatgtgtta aatatttaaa
    19201 cgggcgtata acacgcccgt tgttttattt atgtggatat tattaatagc atatcgagca
    19261 ta
//
LOCUS       DAEEUE010000079        18160 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.079, whole genome shotgun sequence.
ACCESSION   DAEEUE010000079 DAEEUE010000000
VERSION     DAEEUE010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18160
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.079"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..134)
                     /locus_tag="KLK84_002910"
     CDS             complement(<1..134)
                     /locus_tag="KLK84_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE2010160.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     "
     gene            complement(236..1216)
                     /locus_tag="KLK84_002911"
     CDS             complement(236..1216)
                     /locus_tag="KLK84_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBE2010161.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(1224..1934)
                     /locus_tag="KLK84_002912"
     CDS             complement(1224..1934)
                     /locus_tag="KLK84_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HBE2010162.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(2151..3974)
                     /gene="typA"
                     /locus_tag="KLK84_002913"
     CDS             complement(2151..3974)
                     /gene="typA"
                     /locus_tag="KLK84_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HBE2010163.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            4347..5756
                     /gene="glnA"
                     /locus_tag="KLK84_002914"
     CDS             4347..5756
                     /gene="glnA"
                     /locus_tag="KLK84_002914"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HBE2010164.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            5929..6978
                     /gene="glnL"
                     /locus_tag="KLK84_002915"
     CDS             5929..6978
                     /gene="glnL"
                     /locus_tag="KLK84_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HBE2010165.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            6990..8399
                     /gene="glnG"
                     /locus_tag="KLK84_002916"
     CDS             6990..8399
                     /gene="glnG"
                     /locus_tag="KLK84_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HBE2010166.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            8511..8621
                     /locus_tag="KLK84_002917"
     CDS             8511..8621
                     /locus_tag="KLK84_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HBE2010167.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8850..10223)
                     /gene="hemN"
                     /locus_tag="KLK84_002918"
     CDS             complement(8850..10223)
                     /gene="hemN"
                     /locus_tag="KLK84_002918"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBE2010168.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFEEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(10412..10921)
                     /gene="yihI"
                     /locus_tag="KLK84_002919"
     CDS             complement(10412..10921)
                     /gene="yihI"
                     /locus_tag="KLK84_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HBE2010169.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            11503..12135
                     /locus_tag="KLK84_002920"
     CDS             11503..12135
                     /locus_tag="KLK84_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBE2010170.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(12517..15303)
                     /gene="polA"
                     /locus_tag="KLK84_002921"
     CDS             complement(12517..15303)
                     /gene="polA"
                     /locus_tag="KLK84_002921"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBE2010171.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            15667..16599
                     /locus_tag="KLK84_002922"
     CDS             15667..16599
                     /locus_tag="KLK84_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBE2010172.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(16640..18070)
                     /locus_tag="KLK84_002923"
     CDS             complement(16640..18070)
                     /locus_tag="KLK84_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HBE2010173.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         4379 a         4586 c         4373 g         4822 t
ORIGIN      
        1 atttgaccat tggtcaaatg gaaatattcc tgcatcggga tataaaacgc atctttcagc
       61 gacggtaatt tataaattgt accgccacac agtgttaata gactaaataa cgcccatttc
      121 tttttcgtga gcataacatc ccctaagcga tttttaaaat cactgtttta aaggcaataa
      181 gataccgacc attcatatgg aatggtttta ttaactgcgt atgagttaaa acttattagt
      241 ggttcagaag atggtttgcc tcttgtttga tttgttgtag tacgttacgc acatgattta
      301 tctgattaat tgcatcttct ttttctttac gtaaccgcgc atccagtaaa tgattttctg
      361 gtaggggtgt ttcactcatc tggcgataag ggatccacgg attatcatct tcgtcttcaa
      421 agcggaacgc cgacgcatag taatcaacct cttcttccag gccatatgcc gtcacctgcg
      481 gctcatcatc gccaaggcag ataaggtgcg ttaataacgc tttgaatggc atatcaccct
      541 gacctgaaat acaggcttta tgacccaggc caccctgttc tttaacgatc aaggcatctt
      601 taatatggac ctgggtaata tgcggtgcca tcgtttttaa ggcgtcgatg ggatgctcat
      661 ttgcattgat catgttagca aaatcaaaca gtaaggaaag agactccatc tcactttctt
      721 tgaccagcga caccagctca tgacttttta aatcttcatg ctgctcaatc gtaaaagtca
      781 ggccgctgtc ctgatacgtt tcccgtacat aggcaatgtc gttagcgata atcgataata
      841 cgtcgcgcaa attaccttca taacgtggat aaaaacgtac ggacgttgcc ccagttttca
      901 acgcgatggc gacggcttcg tcaatagatg ccttatctga agcgctggtt tcaatatgaa
      961 tatcaaggtt cagacggcgg gctttatccc caaaggcaga gagttcttta tcgtccatat
     1021 taccaagaga aaaacgctcg ccatccagaa catggatttt gacgccacgt agattatttt
     1081 cacttgcgat atcaagtaaa tcagcaggca acactctttc cagccgcata tttaaatgaa
     1141 aggcataagc atgtaaataa agaggaagag tgtcgacacg ttgtagaatc tttcttgcgt
     1201 tatttattgt caccataaaa cacctacaga ttaatgatga ggtctacggc ctcaaatcgc
     1261 caatattcaa tatctatctg gacaatattg ccttgctggt cggcgcgatg cttttcaatt
     1321 aagatcgctg gcatacccgc agaaccgcca agatacttgc tgatatcgcc cggcattctg
     1381 acgggtttga aaatcaattt ttttactacc gtctcttttt ggtagcactt ttcccagact
     1441 gcagaaaatg agtggttttc aagttgttca ataaaacccg gagcaacttc aggattaata
     1501 tatgtttcgt gatagaaaac tttatgtcct tccagcgccc cccagccggt gatgcgatag
     1561 agttcagttg atggcgtcac tgcaatcagt ggcgcgagcg tctcgggaat atcgctggta
     1621 cggtttttct cggtaaaccc ccaagaaggc tctcgccctt gctcaattgc ggcccgctga
     1681 aagctcgccg acagctccgg actgtaatta aatcgcggct gggtcacgaa ccacccctta
     1741 cgatccttac ggaagatttt ggattctgcc tcgaggttca acaacgcctg gcgcagcgtc
     1801 atacgtttaa tgcccagcag ttctcccagc tctctttctg aagggagttt tccacccggc
     1861 gtggtgatgc catctttcaa ccaccgatcc agtttctctt ttgcattttc tacggtggat
     1921 tgattctccg ccattgttta atccttttgg tttaaaccag atgaagcatc ttacgcttac
     1981 gccaaaaaag aaagcaatta acggtgtatg actttgcaga taattgtgac aaagctctct
     2041 attgacgtaa tcacagactt ataagggaga tactcgcatt ggtgtgcgct cacgacaggt
     2101 aaaaaaaaac ctgccatcgc tggcaggttt tttatgacta aaaaacgaaa ttaatcgtct
     2161 ttcggtgcgc ggttggcgcg gcgacgatcg ttttccgtca ggtgacgttt acgaatacgg
     2221 atagaggtcg gagtcacttc taccagttcg tcatcatcga tgaactccag agcttgttcc
     2281 agagtcatgc ggataggcgg aaccagaaca acagcttcgt cagtaccgga agcacgcatg
     2341 ttggtcagtt tcttaccggt caggcagttt acagtcaggt cgttagagcg gctatgaata
     2401 ccgataatct gaccttcgta aacttctgca ccgtgaccga ggaacagctt accgcgatcc
     2461 tgcagaccga acagcgcgaa cgcgacagct ttaccctgac cgttagagat cagtacaccg
     2521 ttctgacgct gacccacttc gcccggacgt acgtcgtcgt agtggctgaa ggtggagtac
     2581 agcagaccag taccggaagt catggtcatg aactcagaac ggaagccaat cagaccacgg
     2641 cttgggatca cgtagtcgag acgtacgcgg cctttaccgt ctggattcat gtttttcagg
     2701 tcgcctttac gttcgcccag cgcctgcatc acagaaccct gatgctgttc ttcaacgtcc
     2761 agcgtcacgt tttcatacgg ctcttgttta cgaccgtcta tttcacggaa gattactttc
     2821 ggacgggata ccgccagttc gaaaccttca cgacgcatgt tttcgataag aacagagagg
     2881 tgcagttcac cacgaccaga aacgcggaac gcatcggcgt cttcggtttc ttctacgcgc
     2941 agcgcaacgt tgtgtaccag ttctttgttc agacgatcga ggatctgacg agacgttacg
     3001 aacttacctt ctttaccgca gaacggcgag gtgttaacgc agaagaacat agaaacggtc
     3061 ggctcatcaa cggagagtgc cggcagcgct tcaacgtttt gcgtgtcgca aacggtgtca
     3121 gaaatgttca gttcgcccag gcccgtgatc gccacgatat cgccagcttc cgccagatcg
     3181 gtttcgatac gttccagacc gaggtggccc agcactttac cgactttcgc gttacgggtt
     3241 ttgccttcgc tatcgatgat agtaacctgc tggttcggct tcactttacc acgcttgatg
     3301 cggccaatgc cgataacgcc aacatagctg ttgtaatcga gctgagaaat ctgcatctgg
     3361 aacggaccgt caaggtcaac gtccggcgca ggaacgtggt caacaatcgc ctgatacagc
     3421 ggggtcatgt cttccgccat atcttcgtgg tccagacccg cgataccgtt cagcgcagaa
     3481 gcgtaaacga tcgggaagtc cagctgctcg tcggtcgcgt cgaggttaac gaacagatcg
     3541 aatacctgat ccacaaccca atcaggacgc gcgccagggc ggtcaacttt gttgataaca
     3601 acaatcggct tcaggccgta agcaaacgct tttttggtta cgaagcgcgt ttgcggcatc
     3661 gggccgtcaa atgcgtcaac caccagcagc actgagtcta ccatggacat tacacgttca
     3721 acttcaccac cgaagtcggc gtgccccggg gtatcaacga tgttgatacg gtaatcattc
     3781 catttgatag cggtgttttt cgcgaggatg gtaatcccac gctctttctc caaatcgttg
     3841 gagtccatca cgcgctcttg ggtttcggca cgagagtcga acgtaccgga ttgttggagc
     3901 agcttgtcta ccagggtggt tttaccatgg tctacgtgcg cgatgatggc gatattacgc
     3961 aatttttcga tcacaacttt gcctcaggca ttagaaatag cgcgttattg tacacggatt
     4021 aatcgcacta caaaacagga tcacaaacat cctccgcaaa caagtattgc agagtccctt
     4081 tgtgatcgct ttcacggagc ataaaaaggg ttatccaaag gtcattgcac caatatggtg
     4141 cttaatgttt ccattgaagc actatattgg tgcaacattc acatcatggt gcagcccttt
     4201 tgcacgatgt taagcatgat aacgcctttt aggggcaatt taaaagttgg cacagatttc
     4261 gctttatctt ttttacggcg acacggccaa aataattgca gatttcgtta ccacgacgac
     4321 aatgaccaat ccaggagagt taaagtatgt ccgctgaaca cgtactgacg atgctgaacg
     4381 agcacgaagt gaagtttgtt gatttgcgct tcaccgatac caaaggtaaa gaacaacacg
     4441 tcactatccc tgctcatcag gtgaatgctg aattcttcga agaaggcaaa atgtttgacg
     4501 gctcctcgat tggcggctgg aaaggcatta acgaatccga catggtgctg atgccagacg
     4561 catccactgc agtgattgac ccgttcttcg ccgactccac cctgattatc cgttgcgaca
     4621 tccttgaacc tggcaccctg caaggctatg accgtgaccc gcgctctatt gcgaagcgcg
     4681 ccgaagatta cctgcgttcc actggcattg ccgacaccgt actgttcggg ccagaacctg
     4741 aattcttcct gttcgatgac atccgtttcg gatcatctat ctccggttcc cacgttgcta
     4801 tcgacgatat cgaaggcgca tggaactcct ccacccaata cgaaggtggt aacaaaggtc
     4861 accgtccggc agtgaaaggc ggttacttcc cggttccgcc ggtagactct gctcaggata
     4921 ttcgttctga aatgtgtctg gtgatggaac agatgggtct ggtggttgaa gcccatcacc
     4981 acgaagtagc gactgctggt cagaacgaag tggctacccg cttcaatacc atgaccaaaa
     5041 aagctgacga aattcagatc tacaaatatg tcgtgcataa cgtagcgcac cgcttcggta
     5101 aaaccgcgac ctttatgcca aaaccgatgt tcggtgataa cggctccggt atgcactgcc
     5161 acatgtctct gtctaaaaac ggcgttaacc tgttcgcagg cgacaaatac gcaggtctgt
     5221 ctgagcaggc gctgtactac attggcggcg taatcaaaca cgctaaagcg attaacgccc
     5281 tggcaaaccc gaccaccaac tcttataagc gtctggtccc gggctacgaa gcaccggtaa
     5341 tgctggctta ctctgcgcgt aaccgttctg cgtctatccg tattccggtg gtttcttctc
     5401 cgaaagcacg tcgtatcgaa gtacgtttcc cagatccggc agctaacccg tacctgtgct
     5461 ttgctgccct gctgatggcc ggtcttgatg gtatcaagaa caagatccat ccgggcgaag
     5521 ccatggacaa aaacctgtat gacctgccgc cagaagaagc gaaagagatc ccacaggttg
     5581 caggctctct ggaagaagca ctgaacgaac tggatctgga ccgcgagttc ctgaaagccg
     5641 gtggcgtgtt cacggatgaa gcgatcgatg cgtacatcgc tctgcgtcgc gaagaagatg
     5701 accgcgtgcg tatgactccg catccggtag agtttgagct gtactacagc gtctaatagt
     5761 tgaagttgta ctacccggcg cacaacgccg ggatttagtt gccgtggaaa ctttcagccc
     5821 atctctgcat gggctttttt ctccgtcaat tctctgatgc ttcgcgcttt ttatccgtaa
     5881 aaagctataa tgcactaaaa tggtgcaacc tgttcaggag actgctttat ggcaacaggc
     5941 acgcagcccg atgctgggca gatcctcaac tcgctgatta acagtatttt gttaattgat
     6001 gacaatctgg cgatccatta cgccaacccc gccgcgcaac aactgctcgc ccaaagctcc
     6061 cgcaaattgt ttggtacgcc gttaccggaa ctgttgagct acttctcatt aaatatcgag
     6121 ctgatgcaag aaagtctgga ggcggggcaa ggctttaccg ataacgaagt gacgctggtc
     6181 atcgatggtc gctcgcatat cctttctgtg acggcccagc gtatgccgga cggcatgatc
     6241 ctgctggaga tggcaccgat ggataaccag cgccgcttaa gccaggaaca actacaacat
     6301 gctcaacaag tagctgcccg tgatttagtg cgcggcctgg cacatgagat taaaaatccg
     6361 cttggcggtt tacgtggcgc ggcgcagtta ctcagcaaag cgttacctga cccatcacta
     6421 ctcgaatata ccaaagtgat tatcgaacag gcggaccggc tgcgaaattt ggtcgaccgt
     6481 ctgttggggc cgcagctgcc cggtacgcgc gttaccgaaa gtattcacaa agtggctgaa
     6541 cgcgtggtaa cgctggtgtc gatggaactg ccggacaacg tgcggttgat tcgtgattac
     6601 gatcccagcc tgccggaact ggcgcacgac ccggatcaaa ttgaacaggt cttgctgaat
     6661 attgtgcgca atgcgctaca ggcgctgggg ccggaaggcg gtgaaatcat tctgcgtacc
     6721 cgcaccgcgt ttcaactgac cttacacggc gagcgctacc ggctggcggc gcggattgat
     6781 gtggaagata acgggccggg cattccgcct catttgcagg atacgctgtt ttacccgatg
     6841 gtcagcggcc gcgaaggtgg caccgggctt ggcttatcca tcgcccgtaa tttgattgat
     6901 cagcattcag gcaaaattga atttaccagt tggccaggtc ataccgagtt ctcggtttac
     6961 ctgcctatca ggaaataaag gtgacgttta tgcaacgagg gatagtctgg gtagtcgatg
     7021 acgatagttc catccgttgg gtgcttgaac gtgcgctcgc tggagcgggt ttaacctgta
     7081 cgacatttga gaacggcgcg gaagtactgg aggcgctggc gagcaaaacg ccggatgtgc
     7141 tgctttcaga tatccgtatg ccgggaatgg acgggctggc gctgctcaag cagattaaac
     7201 agcgccatcc aatgcttccg gtcatcatta tgaccgcaca ttccgatctg gatgctgccg
     7261 tcagcgccta tcaacaaggg gcgtttgatt atctgcccaa accgtttgat atcgacgaag
     7321 cagtggcgct ggttgagcgc gctatcagtc attaccagga acagcagcag ccgcgtaata
     7381 ttcagcttaa cggcccaacg accgatatca tcggcgaagc gcaagccatg caggacgtgt
     7441 tccgtattat cggtcggctt tcgcgttctt ctattagcgt gctgattaac ggcgaatccg
     7501 gcaccggtaa agaactggtc gctcatgccc tgcatcgcca cagtccgcgc accaaagcgc
     7561 cgtttatcgc gctgaatatg gcagctatcc caaaagattt gatcgaatca gaactgtttg
     7621 gccacgagaa aggcgcgttt actggcgcga ataccattcg tcaggggcgt tttgaacagg
     7681 ccgatggcgg cacgttattt ctcgacgaaa ttggtgatat gccgctggat gtgcagacgc
     7741 gtttgctgcg cgtgctggca gacggtcagt tttatcgcgt tggcggctat gcgccggtga
     7801 aagtggatgt gcggattatc gctgccactc accagaatct tgaacagcgg gtgcaggaag
     7861 gtaagttccg tgaggatctg ttccaccgcc tgaacgttat ccgcgttcat ctgccgccgc
     7921 tgcgcgaacg tcgggaagat attccccgtc tggcgcgcca ttttttacag gttgccgcgc
     7981 gcgaactggg cgtagaagcg aagttgctgc atccggaaac cgaagctgct ctgacgcgtc
     8041 tggcgtggcc aggcaacgtg cgccagctgg aaaacacctg ccgctggcta acggtgatgg
     8101 ccgccgggca ggaagtgttg attcaggatt tgcccggcga actgtttgaa tcaacggttg
     8161 cggagagtac ttcgcaaatg caaccggaca gttgggcgac acttttagcg cagtgggcag
     8221 acagagcgct gcgttccggt catcaaaatc tgctttccga agcgcagcca gagctggagc
     8281 ggacgttact gacgaccgcg ttgcgacata cgcaggggca taaacaggaa gcggcgcggc
     8341 tactcggctg gggccgcaac actctgacgc gtaagttaaa agagctgggg atggagtgat
     8401 tcacggcttg tgtgtaaaga ttgattattg agcgcaaatt gctggtattt tgcgctttac
     8461 tgttccgata agttcagtat gatcttgccc ggaaaacggg gagagtcatt atgctggaat
     8521 caataattaa tctggtatcg agtggcgcag ttgacagcca cacaccgcaa actgccgttg
     8581 ctgccgtgct gtgtgccgcg atgattgggc tgtttagctg aagctcgttg tttatgccgg
     8641 atgcggagaa agtcgtaggc ctgataagac gcgaccagcg tcgcatcagg catcatgctc
     8701 agatgccaga tgcggcgtaa acgccttatc cggcctacaa aagcaataat ttcaacaggt
     8761 tttgttgccc agcgcatcag gcaatcttgt tctcagcaca ttgatggcgc tttcgttttc
     8821 tactttgtaa acgaagcgcc attcactctt taaatcaccc gtgagaactg ctgcatccgc
     8881 gctttctggc gcagataggt atcaaagcac atgcaaatgt tgcggatcag caagcgacct
     8941 ttcgccgtca cctgtattcc cttctcatcc acatccacca gcccatcttt tgctaacggg
     9001 gcgagcagct tgagatcttc ctcaaagtaa tcagcgaagt gcaaatccca ctgtttctca
     9061 agaggggcgt aatccagacg gaagttgcag atgagcgact taatcacatc gcggcgaata
     9121 cagtcatcac gcgttagcgc aataccacgc cacagcgcat tgccttgttc atacacttgc
     9181 tgatagtact gcttcaactc tttctggttc tgcgcgtagc agtcgccaat catgctgatg
     9241 gcggaaacgc ccatccccag cagatcggta tcgccctgag tggtgtagcc ctggaagtta
     9301 cgatgcagca cgccttcacg ctgggccacc gccagctcgt catcaggacg ggcaaagtga
     9361 tccataccga taaactgata gcccgattgc gtcaggaagg cgatggtttc ctgcaggata
     9421 tcgagttttt gctgcggact cggcaggtca gcatctttga ttttgcgctg agcagcaaaa
     9481 atggtcggca gatgcgcgta gttaaaaacg ctcagacgat cggggttcag ctccgccaca
     9541 cgtttcaggg taaaggcgaa actctccggc gtctgtttcg gcaggccgta aatcaggtcg
     9601 atgttggtgg aggtaaaacc aatctcacgc gcatggttaa gcagtgcaaa gatgaactct
     9661 tcatcctgct cgcggttaac cagacgttgc acttctttgt tgaagtcctg cacgcccatg
     9721 ctcaggcgat taaagccttc ggcgcgtaaa tgatcgagta catccagttc gatttcccgc
     9781 ggatcgactt cgatcgaaat ctccgcatcg gtattgaaat ggaagttttc gcgcagcagc
     9841 ttcatcagac ggctgatttg cgctttattc agatacgtcg gcgttccgcc gccccagtgc
     9901 aattggctga cgtgacgccc ggcgaacagc ggtgcacgat ggacgatttc ttgctccagc
     9961 gcgtccagat actgatcggc cttgtgctgc tggcgagtaa caatcttatt gcaaccgcag
    10021 aagtaacaaa gcttatggca gaacgggata tgtacgtaga gagataatgg acgctcagga
    10081 tagcgcgcca cggcttgtaa aaacgcctgt tcgccgaagt cttctgaaaa ctccagcgcg
    10141 gtcgggtacg aggtgtatcg tggcccggaa tagttatatt tctggatcag ggccagatcc
    10201 cagtcgattt gctgtacaga catgctcact ccttccgatg gcgtctctgg cggctacggc
    10261 gtaccggcgg atgcccgttt cgggtcatca gccggttgcg cagccacttt tgtcgccgcg
    10321 acaaccgctg tagtttaacg aataaccaca ccagataaca tataaccgga agggttatca
    10381 gcagaacggg aaggcccacg gcggcaatcc gttagttgcc ccgcagcaga cgcatcatgt
    10441 cttcttgctt ctcgtcttct tcctcttctt cgtcatcgtc ataagagagg ccgagtttct
    10501 gcatcaactc atcaatacga tccagtttag catccaccca ggattgctct tcggcgctca
    10561 gggtttcgcc tgcttccaga cgttccagca gcgcatccag acgctcatcc gtttccagta
    10621 attccaactc cgcctgcggt gaaagcatag gtttctcact cttcggtttg tgctgtttgg
    10681 tgactttttc agtcactccc aatggaatag gggttttact gccaatacgt ggatcttttg
    10741 gtgcgttctg gcctttgctg cctgacgtgg tgtttccgcc cgctgcgcgg ctgcccggcg
    10801 catgaccacg acgttttttc tgacgcttac ggtcacgagc ttcctgatcc agctcctcgc
    10861 gtgtttttcg acgcgctttt gcatgacctt tgctgcgtga gtttgaagat gatggtttca
    10921 taatggttcg tcttttagcc gttttattca gtatagattt gcggcggaat ctaacagaaa
    10981 gcaagcaaag aaaaaaggcg acagattact ctgtcgcctt ttttcctgac tcataaccct
    11041 taacgggtct tacaatcctt gcaggccgcc aacacaccct gtttttccat tagccggaac
    11101 aatccgtgtt gattccattt ccgtttaatt acgtctccgg acgtttgtct tcctgacaat
    11161 cctctgtctt cgcctcctgg cgctcctgac cctcatcctg agtcattgtt cctgttagcg
    11221 tcctcgctct atgggtgcta ctttacgcct ttgctttaaa caaacaatca accgccatca
    11281 gtatttgcac attttcagag ttttatataa aagcaattca ctgatttata tagcttattt
    11341 gacatgcatc gcagaatttt ctctgctatt tcgcataaag actatcggca atgtctcaca
    11401 aagcgtatgg cttttactta caacgctcac gccgaaaaat ccgcctttta ttatggttca
    11461 ggtataatcg ccacaaagct ttgactgacg gagacgaccg ctttgactaa tttgaattat
    11521 caacagacgc attttgtgat gagtgcgcct gatattcgcc acctaccttc cgataccgga
    11581 attgaagtgg cttttgcagg ccgttccaac gcaggtaaat ccagcgcgct gaacacgctg
    11641 actaaccaga aaagcctggc tcgtacctca aaaaccccag ggcgcaccca gcttatcaac
    11701 ctgtttgaag tggctgacgg caagcgtctg gttgacttgc ctgggtacgg ttatgcggaa
    11761 gtcccggaag agatgaagcg caaatggcag cgtgcgctcg gcgaatacct cgaaaaacgt
    11821 cagagcctgc aaggtctggt ggtgctaatg gatattcgcc atccgctgaa agatttggat
    11881 cagcagatga ttgagtgggc ggtagacagc aatatcgccg ttctggtgct gctgaccaaa
    11941 gcggacaaac tggcaagcgg cgcacgtaaa gcgctattga atatggtgcg tgaagctgta
    12001 ctggcgttta acggtgatgt gcaggttgaa acgttttctt cgttgaagaa acaaggcgta
    12061 gacaagctga ggcagaaact ggatacctgg tttagcgaga tgcagcctgt agaagaaacg
    12121 caggacggcg aataattttc ttgccttaat gcttgtgccg gatgtggcgt atccggcccg
    12181 taaattcaat agattacaat tctaccgttg gcctgataaa catagcgcat caggcattac
    12241 ggatcttttc tttcgcccaa taaaaaacgc cccagtcatt actgactggg gcggctaaaa
    12301 tattcagcca aatccgatta cgtgaagtaa aaggtctgaa agatagaaca tcttacctct
    12361 gtaccctacg cgactaactt tactcttttt tgttcagttc agaaagcact ttttgtaatt
    12421 ttttttcagt tgttgcatag gaatttctaa tagccatcac aaaacgtgac agcttatgtt
    12481 gcttacttac gaaaaaaggg catgttcagg cgaatcttag tgcgcctgat cccagttttc
    12541 gccactcccc acttccacca gcaacggcac atccagacgg gtacagtttt ccatcagttg
    12601 atgaatctgc ttcgcgacgg catcaacatc atctttatga acttcaaata ccagttcatc
    12661 gtgtacctgc atgatcatac gtacacgcgg ttgctcagcc tgtaaccacg catcaacggc
    12721 aatcatcgcc cgtttgataa tgtcggcggc ggttccctgc attggcgcgt taatggctgc
    12781 acgttcagcc gctgcacgac gagcaccatt gctggatttg atatccggca gatacagacg
    12841 gcgtccgtcc agcgtttcaa cgtagcccag ctctttcgcc tgagcacggg tgcgttccat
    12901 atactccagc acgccagggt agcgttcgaa gtaaaggtcc atgtacttct gcgcttcttt
    12961 acgtggaatg ttcaattgcc gcgccagacc gaaagcactc atgccataaa tcagaccaaa
    13021 gttgatcgct ttcgcgctac ggcgttgctc gctggtgacg gtttccagtg gcaaaccaaa
    13081 cacttctgcc gccgttgccc ggtggatatc ttttccttcc gcgaatgcgg tcagcaagcc
    13141 tttatcacgc gagagatgcg ccataatgcg cagttcaatc tgcgagtagt ccgctgagac
    13201 aatcacataa tcctctggcg caataaacgc ctggcggata cgacgacctt cttcgttacg
    13261 caccggaatg ttttgcaggt taggatcggt tgacgataaa cgtcccgttg cagttactgc
    13321 ctggtgataa gaggtatgca cacgcccggt tttcgggttg atcatcagcg gcagcttgtc
    13381 ggtgtaagtc gacttcagct tcgccagacc acgatactcc agaatcactt ttggcaacgg
    13441 atagtccagc gccagttctt ccagtacctc ttccgacgtt gacggcgcgc cacccggcgt
    13501 tttcttcagc ggtttaatac cctgtttttc aaacagaatg gtttgcaact gcttggtgga
    13561 agaaaggtta aattcttcac ctgcaatttc atgcgctttc ttttccagct cagccagacg
    13621 caaggtgagc tcttcagaat gattgtgcag cactttcgga tcgatcttca caccgttacg
    13681 ttcaatgcgt gaaagcaccg gcaccagcgg catttcgata ttctcgaaga cgttcaacgg
    13741 ccctttgtgt ttctgcagat ccggccacat tttcagatgc aactgcaagg tgacatctgc
    13801 atcttcggcg gcgtaacgtc cggcctcttc gagggcaatc tggttaaagg tcagttgatt
    13861 tttgccttta cccgcaatct cttcaaaagt gatggttttg tgcttcaacc aacgttccgc
    13921 gaggctgtcc atatcgtgac gcccggcaac gctattgaga atgtaggact ccagcatggt
    13981 atcaaacgca atcccacgca gttcaatgcc gtaattcgcc agaataccgc gatcgtattt
    14041 caggttttgc ccgaccttca gcgccttttc atcttccagc agcggtttta gcaactcgag
    14101 tgcacgctcg tgagagattt gatcgggcgc atcaagataa tcatgagcaa ccggaatata
    14161 tgccgctacg cctggctcga tagcaaaaga aagcccgacc aggttagcag agatgttatc
    14221 aaggctgtcg gtttcggtat caaatgcaaa taccggcgct ttttccagct tcgcaatcca
    14281 cgctttcagt gtttcttcat caaggatagt gacgtagttg tcataagaaa tcaccgttgc
    14341 cgtcacttct ggcgcttcgt ctgcaacact ggtttcctgc ggcttcgcgg ctgtttttgc
    14401 ccctttggcc tgtaaccatt tgcccgcttc gacatcagca gtccagcgtt tgaactcata
    14461 ctttttgaac agccccaaca actcttccgc tgccggttgc tgcacttcca gttgttcaca
    14521 ggtcagctcc agttcaacat cggttttaat cgtcgccaac tggtatgaga gataagcaac
    14581 ttctttgttt tgctcgagct tcgctgccat tgttttcgcg ccacggaagc tcaacccagc
    14641 aattttttct ggctcggcat acagcgtatc cagcccgcca agaccttgca gcaatgcctg
    14701 cgcggttttt tcaccgacgc ccggtacgcc aggaatgtta tcagaggagt cacccatcag
    14761 cgccaggaag tcgatgatca attctggcgg cacgccgtac ttattcacca cctcttccgg
    14821 tccgaggatg gtattcgtca tggtattgat aagcgtaata tttggcgtca ccagctgcgc
    14881 catatcttta tcgccagtgc taatcagcac cggacgcccg gctttttcgg cttcgcgcgc
    14941 cagagtaccg ataacgtcgt ccgcttctac gccagaaacc gccagcagcg gcagtcccat
    15001 cgctttaacc atcgcgtgca gaggttcgat ttgtgctcgc agatcgtccg gcattggcgg
    15061 gcgatgtgat ttgtaatgtt caaacagttc atcacgaaag gtttttccct tggcgtcaaa
    15121 gaccaccgct gcatgcgtcg gtttatattg catgatcaga ctgcgcagca tgttgaggac
    15181 accatacatc gcaccggtcg gctcgcctgc gctgttagtc agcgggggaa acgcgtgata
    15241 tgcgcgataa agataagatg aaccatctac aaggataagt ggattttggg ggatctgaac
    15301 cataatgtcc gtgcctgttt atcagattat gggtaaagga tgccacagac aggatgaaaa
    15361 catgagtttt tcaccgcttt tcgtgaaaat cttttgtaga tctgctggat cgctcgcaaa
    15421 tcgtcatgtg gataactttg tgcataaatt atttacatcg cttttaattt attatcatgc
    15481 tttttacagc atgatgtttg tctttgtttt tcatgcagtt aaaaataaat ccatccctaa
    15541 taattgagat aatgggattt tttctaatgt ggatagatat gaattatttt tctccttaag
    15601 gatcatccgt tatttgggtc attttctgct aaaatctgcg ccttgcgctt ctttagtgca
    15661 ctgtctatgg ctaacctttt gaataaattc attatgacga gaatactcgc tgcgataacc
    15721 cttttgctga gtatcgtatt gaccattttg gtcactattt tttgttccgt cccgatcatc
    15781 attgccggga ttgtaaaact attgctacct gtgccagtca tttggcgaaa ggtttcgcgt
    15841 ttttgtgatt ttatgatgta ttgctggtgt gaaggtctgg cggtattact gcatctgaac
    15901 ccacacctgc aatgggaagt tcacgggctg gaagggttaa gtaagaagaa ctggtatctg
    15961 cttatttgta accaccgtag ttgggcagat attgtcgtac tgtgcgtgct gtttcgtaag
    16021 cacattccga tgaataaata tttcctcaag caacagctgg cctgggtgcc atttcttggc
    16081 ctggcgtgct gggcgctgga tatgccgttt atgaagcgtt attcccgcgc ttatttatta
    16141 cgtcatccgg agcgacgtgg tcaagatgtt gaaaccactc ggcgttcttg tgaaaagttt
    16201 cgcctgcatc ccaccaccat tgtcaatttc gttgaaggct cccgtttcac gcaagaaaaa
    16261 catcagcaaa cccactccac ttttcaaaac ttgttgccac caaaggcggc aggcattgcg
    16321 atggcgctaa atgtactggg taaacaattc gataaactgc ttaatgttac gctgtgttat
    16381 ccggacaata accgtcagcc gttcttcgat atgttaagcg gtaaactgac gcggattgtc
    16441 gtacatgtgg atttacagcc tattgccgat gagttacatg gcgactacat caatgacaaa
    16501 agctttaagc gccattttca gcaatggctg aactcattgt ggcaggaaaa agaccggctt
    16561 ctgacgtctc tcatgtcatc acagcgtcag aataagtaag ttttactcgc ctctgaatga
    16621 gcagaggcga gtgagtattt taatgaacaa aacgtccggc acgagacata aattcttctt
    16681 cgctcatctc ctgtccgtta aaaacaactt tacccggtgt ataacgcagc tgcagcgagg
    16741 cggtattgtt gtcaatcgtg attaactgaa acatctgacc taatgtcatc atcccgctga
    16801 tttgtttatc agcctgcttt tgcgcttttt ccgcatccat tccttcagat aaattaagct
    16861 gttttgcggt ttctgtgacg acattaagcg gtaacttcac atcaaaattg agcgatttga
    16921 tatctttgtt tgtggatgat gaagatttgg ctgggtcggc aatactgata tccagattgg
    16981 catttagttc gccgagtgcg ttcttccatc ttaccggttg tttaatggtc ggctcacttt
    17041 tttgtaataa cggtaaatac tctttgaaca atgcagcatt cacttcttgc agagcaactt
    17101 catcgtttgc taactcaggg tgtgcagcaa gctgcttctg catcgcaatg ttatactgga
    17161 tgataaattg gcgcactgct gagggatcaa tagattcagc aatcaatgac cactcaccac
    17221 taccgagttg ctgattccct tttgttaact tatcaagttc gtaggtcaga ttgacattga
    17281 tataatcttt gtctttaacg cgatccagcc tggtttttgc aacgaaagca tcgatttggg
    17341 caacgtcttc cccgtgattg ataatgttaa tctgattaaa tttcgactcg ctttcaccaa
    17401 ccggaaattt cttttcttct aatcgcgcca gagaatcaat ggtaaaactt tttgccgtta
    17461 ctttgttcat tggtgataat tgcagaacaa tatcttcgac tttgccttca atatccaggt
    17521 tagacaatga actaccatca ccattaaaag taaactggcc ctcaccaaaa gaaaattttc
    17581 catactctgc aggggcaaca tctaatgtcg tagagaatga gccaccaaac gcgaatcgca
    17641 atgtcgcttc cacgggcgat ttatttttcg cggcgataaa tagcggttgt gtcagttcat
    17701 tattcactaa gttcactttt gccgctgcca gtgctgggat gacattacca tgcattaaca
    17761 ttgtgatggg caacggaccg tgctcaacat ccacatcaaa tacaactttc tggcctggct
    17821 tgatattgag atcgggtgca ccgttatcga atgtcatttg catttgaaaa cgggaattaa
    17881 aaaagccttt gtcaaagttt ttataactta acgtcatatc gtaggcatgt tcacctttct
    17941 ttttcgcatc gttaaaatct ttaataaact tttcgatacc aggctgaatt tgcgtacctg
    18001 tgtaccatgt gccaccaccc cagattacgg ctaacgcaac aataacacct gtagctgact
    18061 tacgtatcat ataaatattc cctgataaaa cagcagaact ttgaatatcc acttatgctg
    18121 aaataatgtc cagcggcagg atgcattatc agaatgtatt
//
LOCUS       DAEEUE010000080        17893 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.080, whole genome shotgun sequence.
ACCESSION   DAEEUE010000080 DAEEUE010000000
VERSION     DAEEUE010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17893
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.080"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..1546
                     /locus_tag="KLK84_002924"
     CDS             <1..1546
                     /locus_tag="KLK84_002924"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HBE2010174.1"
                     /translation="LTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRN
                     NVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDK
                     QGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDK
                     PAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQ
                     NGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFR
                     NNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAG
                     PGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPL
                     LHRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESP
                     LADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQT
                     AAILMV"
     gene            1705..2264
                     /locus_tag="KLK84_002925"
                     /pseudo
     CDS             1705..2264
                     /locus_tag="KLK84_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            2265..3914
                     /locus_tag="KLK84_002926"
     CDS             2265..3914
                     /locus_tag="KLK84_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HBE2010175.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVTRVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3919..4695
                     /locus_tag="KLK84_002927"
     CDS             3919..4695
                     /locus_tag="KLK84_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HBE2010176.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            4970..7819
                     /gene="rcsC"
                     /locus_tag="KLK84_002928"
     CDS             4970..7819
                     /gene="rcsC"
                     /locus_tag="KLK84_002928"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HBE2010177.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TVMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(8019..8669)
                     /gene="rcsB"
                     /locus_tag="KLK84_002929"
     CDS             complement(8019..8669)
                     /gene="rcsB"
                     /locus_tag="KLK84_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HBE2010178.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(8686..11358)
                     /gene="rcsD"
                     /locus_tag="KLK84_002930"
     CDS             complement(8686..11358)
                     /gene="rcsD"
                     /locus_tag="KLK84_002930"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HBE2010179.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            12096..13217
                     /gene="ompC"
                     /locus_tag="KLK84_002931"
     CDS             12096..13217
                     /gene="ompC"
                     /locus_tag="KLK84_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HBE2010180.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            13329..14384
                     /gene="apbE"
                     /locus_tag="KLK84_002932"
     CDS             13329..14384
                     /gene="apbE"
                     /locus_tag="KLK84_002932"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HBE2010181.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            14458..15522
                     /gene="ada"
                     /locus_tag="KLK84_002933"
     CDS             14458..15522
                     /gene="ada"
                     /locus_tag="KLK84_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HBE2010182.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            15522..16172
                     /gene="alkB"
                     /locus_tag="KLK84_002934"
     CDS             15522..16172
                     /gene="alkB"
                     /locus_tag="KLK84_002934"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HBE2010183.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            16248..17891
                     /gene="yojI"
                     /locus_tag="KLK84_002935"
     CDS             16248..17891
                     /gene="yojI"
                     /locus_tag="KLK84_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HBE2010184.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         4317 a         4359 c         4823 g         4394 t
ORIGIN      
        1 attaacctcg tcgctgaaag gtaaacctgt ttctgcgcag ctaacggtag gcgtggtcga
       61 tgaaatgatc tacgcgctgc aaccagaaat cgcgccgaat atcggcaaat ttttctatcc
      121 gctggggcgt aacaatgtgc gtaccagctc cagtctgtcg tttatcagct atgaccaggc
      181 gctctctagc gagccggttg cgcctggcgc gactaaccgc agcgagcggc gagtaaaaat
      241 gcttgaacgt ccacggcgtg aagaggtgga taccgcggca tggatgccgt cactcacaac
      301 cgataaacaa ggcaaagcgt atttcacgtt cctgatgcct gattcgttaa cccgctggcg
      361 tatcaccgcg cgtgggatga acggcgacgg gctggtcggg caggggcgtg cttatctgcg
      421 ttcggaaaaa aatctctaca tgaagtggag tatgccaacg gtgtatcgcg tgggcgacaa
      481 accggcggca ggactgttta tcttcagtca gcaggataac gaaccggtgg cgctggtgac
      541 taaatttgca ggcgctgaga tgcgccagac gctgacgctg cacaaagggg cgaattatat
      601 ttcgctgacg cagaatattc agcaatctgg cttgttaagt gcagaactgc aacaaaatgg
      661 gcaagtgcag gacagcatta gcacaaaact gtcttttgtg gacaacagct ggcccgttga
      721 acagcagaaa aatgtcatgc tcggcggtgg cgagaacgcg ctgatgttgc ccgagcaggc
      781 gagtaatatc cggctacaaa gtagtgaaac gccgcaggag attttccgca acaatcttga
      841 tgcgttagtc gatgaaccgt ggggtggggt gatcaacacc ggtagccgtc tgatcccgct
      901 cagtctcgcc tggcgttcgc ttgccgatca tcaaagtgcc gccgctaacg acattcgtca
      961 gatgattcag gataaccgtc tgcggctgat gcaactggcg gggcccggag cgcgctttac
     1021 ctggtggggt gaagatggca atggtgacgc cttccttacg gcatgggcat ggtacgccga
     1081 ctggcaggcc agccaggcgc tcggcgtaac gcaacaaccg gaatactggc agcatatgct
     1141 cgacagttat gccgagcagg cggataacat gccgttattg catcgggcgc tggtgctggc
     1201 gtgggcacag gagatgaatc tgccgtgcaa aacgttgttg aaaggggggg atgaagctat
     1261 cgcccggcgc ggaactaaag atgaagattt ctctgaggaa gacatccgcg atatcaatga
     1321 cagcctgatc ctcgatacac cggaatctcc actggcagat gcggtggcaa acgtcttaac
     1381 catgacgttg ctgaaaaaag cgcagttgaa gtccacggtg atgccacagg ttcagcaata
     1441 tgcgtgggat aaagcggcaa acagcaatca gccgctggcg cacacggttg tgctgctcaa
     1501 tagcggtggt gacgctaccc agacggccgc tattttaatg gtttgaccgc tgagcaatcc
     1561 actattgagc gcgcgctggc catgaactgg ctggcgaaat atatggcgac aatgcctcca
     1621 gttgttttgc ctgcgcctgc gggcgcatgg gctaaacata agttaactgg agggggcgaa
     1681 gactggcgtt gggttggtta gggtgtgccg gacattctct cttttggtga cgaattatcg
     1741 ccgcaaatgt gcaggtccgc tggcgtgaac cggcaaaaac ggctcaacaa agtaacattc
     1801 cggtgaccgt tgaacgccag ttgtatcggc ttatccccgg tgaagaagag atgagcttta
     1861 ctctgcagcc ggtgaccagc aatgagattg acagcgatgc gctgtatctc gatgaaatta
     1921 cgcttaccag cgagcaggat gcagttctgc gctatggtca ggtggaagta ccgctcccgc
     1981 cgggagccga cgttgagcgc acaacatggg gcatttcagt caataaaccc aacgccgcga
     2041 aacagcaggg gcaattgctg gaaaaagcgc gtaatgaaat gggcgaactg gcctatatgg
     2101 tgccggtgaa agaactgacg ggaacggtca ctttccgcca tttgctgcgc ttctcgcaaa
     2161 aagggcaatt cgttctgcct cctgctcgtt atgtgcgttc ctatgcacct gcgcagcaaa
     2221 gtgtcgcggc agggagtgaa tggaccggga tgcaggtgaa ataagtgaac tggcgcagaa
     2281 tcgtctggct attggcgttg gttactctgc caacgcttgc ggaggagacg ccgttacaac
     2341 tggtgctgcg cggtgcgcag cacgatcaac tgtatcagct gtcgtcatca ggcgtgacca
     2401 aagtctcagc attacctgac tcgctcacca cgccgttagg gagtttgtgg aaactgtatg
     2461 tttacgcctg gctggaggat acccatcaac cggagcagcc ctaccagtgt cgcggaaact
     2521 ccccggaaga ggtttattgt tgtcaggctg gggagagcat aacgcgcgat accgcgctgg
     2581 tacgttcctg tgggctctat tttgcgccgc agcggttgca tattggcgca gatgtgtggg
     2641 ggcaatactg gcaacagcgt caggcacctg cgtggttagc gtctcttaca acgctgaagc
     2701 cagaaacctc ggtaacggtt aaatcgctgc ttgattcatt agcgacgtta cctgcgcaaa
     2761 acaaagctca ggaagtctta ctggatgtgg tgctggacga ggcgaaaatc ggtgtcgcat
     2821 ccatgctcgg tagtcgggta cgggtgaaaa cctggagctg gtttgccgat gataaacagg
     2881 aaatccgtca gggtggtttt gctggctggt taaccgatgg tacaccgctg tgggttactg
     2941 gcagtggcac cagcaaaacg gtactaaccc gttatgcgac ggtgcttaat cgggttttgc
     3001 cagtgccgac gcaggttgcc agtgggcaat gtgtagaggt ggagctgttt gcccgctatc
     3061 cgctgaagaa aatcacagcg gaaaagagta cgacggcagt aaaacccagc gtacttaatg
     3121 ggcgctatcg tgtgacgttt actaatggta accacattac gtttgtcagc cacggtgaga
     3181 cgaccttact gagcgaaaag ggcaaactga aattgcagtc gcatctcgat cgtgaagagt
     3241 acgttaccag agttctggat cgcgaggcga aaagtacgcc acctgaggca gcgaaagcca
     3301 tgactgtggc gatacgtact tttttacagc aaaacgccaa tcgggaaggt gattgcctga
     3361 ccatccccga cagcagcgcc acgcagcgtg tttctgcttc gccagcgacc acgggggcgc
     3421 gaacgatgac cgcctggacg caggagctca tctatgcagg cgatccggtt cattatcatg
     3481 gcagtcgcgc taccgaaggg acactttcct ggcggcaagc cacggcccag gcggggcagg
     3541 gggagcgtta cgaccagata ctcgcgtttg cttatcccga caacagcctt agccgctggg
     3601 gagctccacg ctcaacctgc cagttattac ccaaagcgaa agcctggctg gcgaaaaaaa
     3661 tgccgcagtg gcggcgtata ttacaaggcg agacggggta caacgaacca gacgtgtttg
     3721 cggtctgtcg tttagtctca ggtttcccct ataccgatcg gcagcagaaa cggctgttta
     3781 ttcgtaactt ctttacgctt caggatcggc tggatttaac ccatgagtat ctgcacttgg
     3841 cctttgatgg ttatcccacc gggcttgatg agaactatat cgaaacgctg acccgacaat
     3901 tattaatgga ctaatgctat gcgaaagatt tttcttccgt tgttactggt ggcactttcg
     3961 cctgtcgctc acagtgaagg tgtgcaggaa gtcgagattg acgcaccgct ctctggctgg
     4021 catccggcag agggcgaaga tgccagtttt tcacaatcga tcaattaccc ggcgtcgtcc
     4081 gtcaacatgg cagatgatca aaatatctca gcgcagatcc gcggcaaaat aaagaattat
     4141 gctgcggcgg gtaaagttca gcagggccgg ctggtggtca acggtgccag tatgccgcag
     4201 agaattgaat ccgatggttc atttgcacgc ccttatattt tcactgaagg cagcaacagc
     4261 gtgcaggtca tcagcccgga tgggcaaagc cggcaaaaaa tgcagtttta ctcaacgccg
     4321 ggtacaggaa ctattcgtgc acgtttacgg ctggttctct cgtgggatac ggacaatacc
     4381 gatctcgatc tccatgttgt cacgcccgat ggcgaacacg cctggtacgg taacaccgtg
     4441 ctgaaaaaca gtggtgcact ggatatggat gtcacgacgg ggtacggacc cgagattttc
     4501 gccatgccag caccagttca cgggcgctat caggtgtata tcaactacta tggtgggcgc
     4561 agcgaaacgg aattgacgac cgcccaactg acgctgatca ccgatgaagg atcggtcaat
     4621 gagaaacagg aaacatttat tgtaccgatg cgtaatgctg gcgagctgac gctggtgaaa
     4681 agttttgact ggtgatccat cccttcacac aacctggctc tctctttttt cagataagac
     4741 tgctgggcga tctgccgacg ccaggatata accattaaca agcgtcggca gattggtcat
     4801 ttcttatttt tcgtactgag aactgtccat ctactccggc tttcctggtt aaggcaatgg
     4861 tttctaagcg atacgcatta ttgcaatggt gacagaacat tgcggtaaca cgcttttacc
     4921 gctaccttaa ccacactcca tcggtcacct gaggcggagc ttctcccctt tgaaatacct
     4981 tgcttctttt cgtacaaccc tgaaagcctc gcgctacatg ttcagagcat tggcgttagt
     5041 gctctggctg ttgattgctt tttcatccgt tttttacatc gttaatgcgt tacatcagcg
     5101 agaatcggaa attcgtcagg aatttaatct gagttccgat caggctcagc gctttattca
     5161 acgcacctct gatgtgatga aagagcttaa gtacatcgcc gaaaatcgct tatcggcaga
     5221 aaacggtgtg ctttccccgc gtggacgaga aacgcaggcg gatgtgcctg cgtttgaacc
     5281 gctgtttgcc gactccgatt gttccgcaat gagtaacacc tggcgaggtt ctctggagtc
     5341 attggcgtgg tttatgcgct actggcgcga taatttttct gcggcttacg atctcaaccg
     5401 ggtattttta atcggcagtg ataacctctg catggccaat ttcggtctgc gtgatatgcc
     5461 agtggaacgc gataccgcgt tgaaagcgtt gcatgaacgc atcaataaat atcgaaatgc
     5521 accacaagat gatagcggca gtaacctcta ctggatcagc gaaggtccgc gcccttgcgt
     5581 cgggtatttt tacgcgttga cgccagttta tctggcgaac cggttgcagg cgcttttggg
     5641 tgtcgagcag accatccgga tggagaactt tttcttaccg ggtacgttgc cgatgggggt
     5701 taccattctt gatgaaaatg gtcataccct gatttcgctt accggaccag aaagtaaaat
     5761 taagggcgat cctcgctgga tgcaggaacg ctcctggttt ggctatacgg aagggttccg
     5821 ggagctggtg ctgaagaaaa atctgccacc ctcatcgcta agcatcgtgt attcggtgcc
     5881 ggttgataag gtgctggaac gcattcgcat gttgatcctt aacgcaattt tgctgaatgt
     5941 gcttgccgga gctgcattgt ttactctcgc gcggatgtac gagcgacgta ttttcattcc
     6001 ggcggaaagc gacgccctgc gactggaaga acatgagcag ttcaatcgca agattgtcgc
     6061 ctccgcgcca gtgggtatct gcattttgcg taccgctgat ggcgtcaata ttttaagtaa
     6121 cgaactggcg catacctatc tcaatatgct tacgcatgag gaccgccaac gactgacgca
     6181 aattatctgt gggcagcagg tcaattttgt tgatgtcctg accagcaaca ataccaatct
     6241 gcaaatcagc ttcgtccatt cgcgctatcg taatgaaaac gtggccattt gtgtgctggt
     6301 ggatgtttct tcgcgcgtga agatggaaga gtcgttgcag gagatggcac aagcagcgga
     6361 acaggcgagc cagtcaaaat cgatgttcct tgccaccgtc agtcatgagc tgcgaacgcc
     6421 gctgtatggc attatcggta acctggatct gttgcaaacc aaagagttac cgaaaggcgt
     6481 cgatcgtctg gtgacggtaa tgaacaactc ttccagcctg ttgttgaaaa ttatcagcga
     6541 tattctcgat ttctcgaaga ttgaatcgga acagttgaag atcgaaccgc gtgagttttc
     6601 accgcgtgaa gtgatgaacc acatcaccgc caactattta ccgctggtgg tacgcaagca
     6661 gttaggcttg tactgcttta ttgaaccgga tgtgccagtg gccttaaatg gcgacccgat
     6721 gcgtttacag caggtcatct ccaacctgtt gagtaacgcc ataaaattca ccgataccgg
     6781 ctgtatagtt ttgcatgttc gcgctgatgg cgattatctc tctatccgtg ttcgcgatac
     6841 cggcgtgggg attccggcga aagaagtggt gcgcttgttt gatcccttct tccaggttgg
     6901 aacgggcgta cagcgaaact tccaggggac cggtctgggt ctggcgattt gtgaaaaact
     6961 gatcagcatg atggacggcg atatctcggt agattcagaa ccgggaatgg gcagccagtt
     7021 taccgtgcgt attccgttgt acggcgctca gtacccgcag aaaaaaggcg tggaagggtt
     7081 gagtggtaaa cgctgctggc tggcggtccg caatgcgtcg ctctgtcagt tcctggaaac
     7141 cagtttgcag cgcagcggca tcgtcgttac aacatacgaa gggcaggaac cgactcccga
     7201 agatgtgtta atcactgacg aggtagtgag taaaaaatgg cagggcagag cggtagtgac
     7261 cttctgtcgt cgccatattg gtattccgct ggagaaagcg ccaggggagt gggtacacag
     7321 tgtggctgct ccgcatgagc taccggcatt gttggcgcgt atttatttga tcgagatgga
     7381 gagcgacgat cctgctaacg ctctgccgtc gacggacaaa gcggtcagcg ataacgacga
     7441 tatgatgatt ctggtcgtgg atgatcatcc gattaaccga cgtctgctgg cagatcagtt
     7501 gggatcgttg ggctatcaat gtaaaaccgc gaatgatggc gtcgatgcgc ttaatgtact
     7561 tagtaagaat catattgata tcgtgcttag cgacgtcaac atgccaaata tggatggtta
     7621 ccgcttgacg caacgcattc gtcagttggg actgacgttg ccggtaatcg gagtaactgc
     7681 taatgcgttg gctgaagaga agcagcggtg tctggagtcc ggtatggaca gctgcctctc
     7741 gaagccggta acgctggatg tgataaaaca gacgctgacg gtatatgccg agagggtcag
     7801 gaaatcgcgg gattcgtagg acggataagg cgtttacgcc gcatccggca ttcagtgcaa
     7861 atgccagata agacactgac gcgtcttatc tggcctactt taatgtaatc tgttgaaata
     7921 atgggaatcg taggccggat aaggcgttta cgccgcatcc ggcaatcggt gcaaatgcca
     7981 gataagacac taacgcgtct tatctggcct acagatgatt agtctttatc tgccggactt
     8041 aaggtcacag aagagagata attcagcagg gcgatatcgt tctcgacgcc cagcttcatc
     8101 atcgcagatt tcttctggct actgatggtt ttaatactgc ggttcagctt tttagcgatc
     8161 tcggtcacta ggaagccttc cgcaaacagg cgcagaactt cactctcttt tggcgagaga
     8221 cgcttgtcac cgtaaccacc agcactgatt ttttccaaca ggcgagaaac gctttccggg
     8281 gtaaatttct tccctttctg cagcgcggcg agagctttcg gcagatcggt cggtgcacct
     8341 tgtttcagca cgatcccttc gatatccaga tccaataccg cactaagaat cgccgggttg
     8401 ttgttcatag tcagaacaat gatcgacagg cttgggaaat ggcgcttgat gtacttgatt
     8461 aaggtaatgc catcgccgta cttatcgcca ggcatggaga gatcggtaat caacacatgc
     8521 gcatccagtt tcggcaggtt gttgatcagt gctgtagagt cttcaaattc gccgacaaca
     8581 ttcacccact caatttgctc aagtgatttg cgaataccga acaagactat cggatggtca
     8641 tcggcaataa ttacgttcat attgttcatg taataggcta ccttgctaca gcaagctctt
     8701 gacataactg tcaatgtcgc tgatgtattt ttctattcct ggaacatcct tctcacgaat
     8761 cagatgttcc agcgtttcac ataactgctt gccgggtacc agatttagca tggcaaatac
     8821 gcctttaaga cgatgagccg tttgggctaa cgcagcaaag tcactggttg ctgcttcagt
     8881 atacagcctc ttaacatcat ccggtactgt gtctacaaag agcgcataat agccgctggc
     8941 atggagttgc gcattttcat ctccgcccag aggcgattct gtcacctctt cctgtgccag
     9001 ttgcacttca attaattgta agaccgcttc ctgcatagcg ttgctcatat tgaagttgac
     9061 gcacaattga ccaggcccaa tttctcgtac gccagactca tcatcgctta aaagcaagcc
     9121 agaggcagta agattagacg gattatccgt taaaaagata tcataatctt gactaattaa
     9181 tctttcatcg ggtgtgatac aggttgcacc ccaattttct aacaggcgag tgacaatatt
     9241 ccgaatttct gccgaagtaa catccaccat tacgcagaca tcatccagta aacgctcttc
     9301 ttcctcttca acttccgggt cagcggcgag cattttgata tgcacagagt agcgtgtacc
     9361 aagcccatcc cgcgttttga tgtttaaatg accgcccagt ttacgtgcca gttgatcgct
     9421 cagccagaat gccagcgggt ccgcctttcc ataacgatcg ttttgggtct ggttgataaa
     9481 cgggaagtgc aaattatcca tttcatgaat acttacgcct tctcccgtgt ccagaatgcg
     9541 gaatgtcagg cggtcttcag aggactcatc ctgatcaacc tcaagggtga tttttcccaa
     9601 ttgcgttgag gtcacggcat attgcatcag tagcagcaaa atacgtcgta aggcatcgcg
     9661 atcgccgcgg cgcatatcgt gtgctttcag atgattgtta atcagcagtt gcagaccttt
     9721 acgcttgatg gcaggcaaca ctgaaggcac aacttcatca attaaatcct gcacggagaa
     9781 cagcaccgtc tcacttttcc agctatcatc cgcaagcatg ttcgctaact gtatttcttc
     9841 gaccagccgc accagcacat ctgcctgatt cgccagttgt ttgctttccg gggcgttgag
     9901 tttagccgca ctctccgcca gggactgtgc gggttctttc agcgcatcgc caatgttttt
     9961 cataaaggtc atccgcccct gctggttttt ctcatacaga cgctgcgcct gcttgagttt
    10021 cttgtttacc agcacttcgc gatcctgatc gcgaataatg aaaatttgtg tgcgcggcgc
    10081 gacctggctg cggaacatgc ggatctcata cagctcgtta ttgatcgtcg cctgaataat
    10141 cccctgatgc tgttccgcca tggtggtgat gttttgcaga ttcagatgcg gcagcaaatg
    10201 atcggcaatt ttgttactta tgacagtgcg gttcgattcc tgatcgtgaa ccagcaggcc
    10261 gagcggcaac agtgagacta tctcttcatt gattgcccgt aaaatgcgca attcgttatt
    10321 gaccgccgtg ctggggacgc tttctgtact gcggctggag aaatggcgga atgtggtata
    10381 gccaaataac gccagcgcca gcaaaccgat gttcagcagc agtggcagca gaatgttttg
    10441 caacgtatcc agcaataagg tgccataagg aacctgccag accaggcgca tatcggtaga
    10501 gttgagtgcc gaggagattt caatcttcgt actgttaaag tggatactga cgctatccgt
    10561 cccttctttc tcattatcat tgtttcccgt tgccgtcgcg tctggctcaa ggcggaaact
    10621 gtccagcggc atacccggtg ggatcaaatc attaatcggc agatcaaaag ccacgaccgt
    10681 tgccagatgt cccggctggt tgaacgtggt acgcaaggta aagtaatgac cgttctgcca
    10741 ggctaggcgg cgcatgttag aaaaactttc gcgttcatcg agggcgttgg cctgttgtaa
    10801 catttctgca cgacgtgaat caacaatgtc actgacggtc gattctttaa atccggaggt
    10861 gagatcttta aggggtaggg ttgagatcag caccagactg ttatcctgac cattcaggta
    10921 atacatcgac cacggtacat tttctgcacc ccacaatgta tccagataag tggacatccg
    10981 ctgagtcatc tcaagcgttg agttgtcgtg agagccaaag attaacgctt ccgttttgcg
    11041 gcgtggtttc tccagatagt agacatcctg tttcaggcgc gtctcttgta aaccttcgcc
    11101 ggaggagggg gaggtcgtcg cggcaatgtt gtcgtagatc tgcctggtca cgtaacgcca
    11161 ggtatcgacg cgcttttgga ttgcgtgggt aatgtcgaca atctggtaac ttttatcttt
    11221 cagccaagcg ttaacggcac tttgtaccat tacccccatc gtcaccagta acacaatgat
    11281 caacagtaaa aagaagcggg taatgctccc cggtaggagt gaaaagcggg tcgtggccgt
    11341 tgtctctttc tgacgcattc gtgtttatga cctgttaaaa cttcgcgaag tgatagggca
    11401 gtataaaggg tacagtgtga aattccagac tcttacctga ttgatgattc tctattctcc
    11461 cgtgatattt atcatcctgt atggggggaa atagatgtac aagcgccatt ttgctgtaca
    11521 aataacctac aaaaagccca acgaaattaa ttgcaaataa acaaagattg ctggaaatta
    11581 tgctgatgtt aattatttgt gaaatagtta acaagcgtta tagtttttct gtggtagcac
    11641 agaataatga aaagtgtgta aagaagggta aaaaaaccga atgcgaggca tccggttgaa
    11701 ataggggtaa acagacattc agaaatgaat gacggtaata aataaagtta atgatgatag
    11761 cgggagttat tctagttgcg agtgaaggtt ttgttttgac attcagtgct gtcaaatact
    11821 taagaataag ttattgattt taaccttgaa ttattattgc ttgatgttag gtgcttattt
    11881 cgccattccg caataatctt aaaaagttcc cttgtattta cattttgaaa catctatagc
    11941 gataaatgaa acatcttaaa agttttagta tcatattcgt gttggattat tctgcatttt
    12001 tggggagaat ggacttgccg actgattaat gagggttaat cagtatgcag tggcataaaa
    12061 aagcaaataa aggcatataa cagagggtta ataacatgaa agttaaagta ctgtccctcc
    12121 tggtcccagc tctgctggta gcaggcgcag caaacgctgc tgaagtttac aacaaagacg
    12181 gcaacaaatt agatctgtac ggtaaagtag acggcctgca ctatttctct gacgacaagt
    12241 ctgtagatgg cgaccagacc tacatgcgtc ttggcttcaa aggtgaaact caggttactg
    12301 accagctgac cggttacggc cagtgggaat atcagatcca gggcaacagc gctgaaaacg
    12361 aaaacaactc ctggacccgt gtggcattcg caggtctgaa attccaggat gtaggttctt
    12421 tcgactacgg tcgtaactac ggcgtagttt acgacgtaac ttcctggacc gacgtactgc
    12481 cagaattcgg tggtgacact tacggttctg acaacttcat gcagcagcgt ggtaacggct
    12541 tcgcaaccta ccgtagcacc gacttctttg gtctggttga cggtctgaac ttcgctgttc
    12601 agtatcaggg caaaaacggt agcccggaag gtgaaggcat gaccaacaac ggtcgtgaag
    12661 ctctgcgtca gaacggtgac ggcgttggcg gttctattac ttatgattac gaaggcttcg
    12721 gtatcggtgc tgcagtttcc agctccaaac gtactgatga ccagaacttc ggtctgaacc
    12781 gttatgatga gcgttacatc ggtaatggcg accgcgctga aacctacacc ggtggtctga
    12841 aatacgacgc gaacaacatc tacctagctg ctcagtacac ccagacctac aacgcaactc
    12901 gcgtaggcaa cttgggttgg gcgaacaaag cacagaactt cgaagctgtt gctcagtacc
    12961 agttcgactt cggtctgcgt ccgtccctgg cttacctgca gtctaaaggt aaaaacctgg
    13021 gtgtcatcaa tggtcgtaac tacgacgacg aagatatcct gaaatatgtt gatgttggtg
    13081 cgacctacta cttcaacaaa aacatgtcca cctacgttga ctacaaaatc aacctgctgg
    13141 acgacaacca gttcactcgt gacgctggca tcaacactga taacatcgta gctctgggtc
    13201 tggtttacca gttctaatct cgatggatat cgaacaaagg gcctgcgggc ccttttttca
    13261 ttgttttcag cgtacaaaca cagttttttg gtgtactctt gcgaccgttc gcatgaggat
    13321 aatcacgtat ggaaataagc tttacccgcg tggcactgct ggttgccgcg ctcttctttg
    13381 ttggttgcga tcaaaaacca caacccgcca aaacccacgc tactgaagtt accgttcttg
    13441 aaggcaaaac catgggtacc ttctggcgtg ccagcatccc gggcattgat gccaaacgca
    13501 gtgccgaact taaagaaaag attcagaccc agctggacgc cgacgatcag ctgctttcga
    13561 cctataaaaa agattccgcg ctgatgcgct ttaacgactc gcaaagtttg tcgccgtggc
    13621 cggtaagtga agcgatggcc gatatcgtca ccacctcgct gcgcattggg gcaaggaccg
    13681 atggtgcgat ggatattacc gtcgggccgc tggtgaatct gtggggcttt ggcccggaac
    13741 aacagccggt tcaaattccg agccaggaac agatcgatgc gatgaaagcc aaaaccggct
    13801 tacagcacct gacggtcatc aatcagtcgc atcagcaata tctgcaaaaa gacctgccgg
    13861 atttatatgt cgatctctcc accgtcggtg aaggttatgc ggcggatcac ctggcacgct
    13921 tgatggagca ggaagggatt tcccgctatc tggtgtcggt gggcggcgcg ctgaacagcc
    13981 gtggtatgaa cggtgaaggc cagccgtggc gggtagcgat tcaaaaacca accgataaag
    14041 aaaacgcggt tcaggctgtg gtggatatca acggtcatgg gattagcacc tctggcagct
    14101 accgtaacta ttacgaactg gacggcaaac gtctttccca tgttatcgat ccgcaaaccg
    14161 ggcgtcccat cgaacacaat ctggtatccg tgacggtgat tgctccgacg gcgctggaag
    14221 ccgatgcctg ggatactggc ttgatggtac tcgggccgga gaaagccaaa gaggttgttc
    14281 gccgggaagg gctggcggtt tatatgatca ccaaagaagg cgatagcttt aaaacctgga
    14341 tgtcaccaca gtttaaaagc ttccttgtca gcgaaaaaaa ttaaagcgca agattgttgg
    14401 tttttgcgtg atggtgaccg ggcagactaa aggctatcct taaccaggga gctgattatg
    14461 aaaaacgcca catgcttaac tgacgatcaa cgctggcaat ctgtcttagc ccgcgacccg
    14521 aatgccgacg gcgaattcgt tttcgccgtg cgcaccacgg gtatcttttg ccgtccttct
    14581 tgccgcgcca gacatgcctt gcgggaaaat gtctccttct acgcaaatgc cagcgaggca
    14641 ctcgccgccg gatttcgccc ctgcaaacgt tgtcagccag acaaagccaa tccccggcaa
    14701 catcggttgg ataaaatcac ccacgcgtgt cgactgctgg aacaggaaac gcctgtaacg
    14761 ctggaagcct tagccgacca ggtggcgatg agtccatttc atctacatcg gttgtttaaa
    14821 gctactaccg gaatgacgcc taaagcctgg caacaggcct ggcgcgctcg ccgtttgcgc
    14881 gaatcgctgg cgaaagggga gagcgtgacg acgtctattc ttaacgccgg attccccgac
    14941 agcagcagtt actaccgcaa agccgatgaa acgctgggca tgacggctaa acaattccgt
    15001 cacggtggcg aaaatctggc ggtgcgttac gcgctggctg attgtgagct gggtcgttgc
    15061 ctggtggcag aaagcgagcg ggggatttgc gcgatattgc tgggcgatga tgacgcgaca
    15121 ttaatcagcg agttgcagca gatgtttccc gctgccgaca acgcgcctgc cgatctgacg
    15181 tttcagcaac atgtgcgtga agtgatcgcc agcctcaatc aacgcgatac gccgctgacg
    15241 ttaccgctgg atattcgcgg cactgctttt cagcaacaag tctggcaggc actgcgcacg
    15301 ataccttgtg gtgaaaccgt cagttatcag caactggcta acgccatcgg caaaccgaaa
    15361 gcggtacggg ccgttgccag cgcctgtgcc gccaacaagc tggctatcgt aataccctgt
    15421 catcgagtgg tccgtggtga tggcacactt tccggttacc gctggggcgt gtcgcgtaaa
    15481 gcgcaactgc tgcgccgcga agctgaaaat gaggagaggt aatgttggat ctgtttgccg
    15541 atgctgaacc gtggcaagag ccactggcgg ctggtgcggt aattttacgg cgttttgctt
    15601 ttaatgctgc ggagcaacta atccgcgata ttaatgacgt tgccagccag tcgccgtttc
    15661 gccagatggt cgctccaggc ggatatacca tgtcggtggc gatgaccaac tgtgggcatc
    15721 tgggctggac gacccatcgg caaggttatc tctattcgcc cattgatccg caaacaaata
    15781 aaccgtggcc cgccatgcca caaagctttc atgatttatg tcaacgtgcg gctacggcgg
    15841 cgggctatcc agatttccag ccagatgctt gtcttatcaa ccgctacgct cctggcgcga
    15901 aactgtcgct gcatcaggat aaagacgaac cggatctgcg cgcgccaatt gtttctgttt
    15961 ctctgggctt acccgcgatt tttcaatttg gtggcctgaa acgtaatgat ccgctcaaac
    16021 gtttgttgtt ggaacatggc gatgtggtgg tatggggcgg tgaatcgcgg ctgttttatc
    16081 acggtattca accgttgaaa gcggggtttc atccactcac caccgactgc cgctacaacc
    16141 tgacattccg tcaggcaggt aaaaaagaat aaaaataaga attattattg ctgtgcgcgc
    16201 gaagatgttt aaactgcggg ctgtaattca ttgtccgggt tttctgcatg gaacttcttg
    16261 tacttgtctg gcggcagtat cgctggccat ttatcagtgt gatggcgcta agcctcgcca
    16321 gtgcggcatt aggcattggc ttaattgctt ttatcaatca gcgccttatc gaaacggcgg
    16381 ataccagtct gctggtgtta ccggagtttc tgggattatt gctgctgttg atggcagtca
    16441 ctctcggatc gcaactggcg ctcaccactt tggggcatca cttcgtttac cgactgcgta
    16501 gtgaatttat caagcggatt ctggataccc acgtcgagcg cattgaacaa ctcggtagcg
    16561 cctcgttgct ggcgggatta accagcgatg tgcgcaatat caccattgct tttgtgcgtc
    16621 tgccggaact ggggcagggg atcattctca ctatcggttc atcggcgtat ctgtggatgc
    16681 tgtcgggcaa aatgttgctg gtaacggcta tctggatggc gatcaccatc tggggcggtt
    16741 ttgtgctggt ggcgcgggtg tacaaacata tggcgaccct acgtgaaacc gaagacaagc
    16801 tgtacacgga ttttcaaact gtacttgaag ggcgcaaaga gctgactctg aaccgggaac
    16861 gcgccgagta tgtgtttaac aacctctaca tccccgatgc gcaagagtat cgccatcata
    16921 ttattcgcgc agacaccttc catcttagtg ccgtgaactg gtcaaacatc atgatgctgg
    16981 gcgctatcgg cctggtgttc tggatggcga acagtctcgg ttgggctgat accaacgttg
    17041 ctgcgaccta ttcgttgacg ctgttattcc tgcgtacgcc gctgctttcg gcggttggcg
    17101 cattgccgac gctgctgacg gcgcaggtgg cgtttaacaa gctgaacaaa ttcgcgctcg
    17161 cgcctttcaa agctgagttt ccgcgcccgc aggcgtttcc caactggcaa acgctggagc
    17221 tgcgtaacgt gacgtttgct tatcaggata acgcgttttc cgttggtccg attaatctca
    17281 ccatcaaacg tggcgagctg ctgtttctga ttggcggtaa cggtagcgga aaatcgacgc
    17341 tggcgatgtt gttgacgggc ttgtatcagc cacaaagcgg cgaaatcttg ctggatggca
    17401 aacctgtcag cggcgaacaa ccggaagatt atcgcaaact gttttcggca gtgtttaccg
    17461 atgtctggct gtttgatcaa ctgctggggc cggaggggca acccgctaac ccgcaactgg
    17521 ttgagaagtg gctggcgcag ctgaaaatgg ctcataagct tgagttaagc aacgggcgta
    17581 ttgttaacct gaagttatca aaagggcaga aaaaacgcgt ggcgctgttg ctggcgctgg
    17641 cagaagaacg cgatattatc ctgctggatg aatgggcggt ggatcaggat ccacacttcc
    17701 gtcgtgagtt ttatcaggtg ttgctgccgc tgatgcagga gatgggtaaa actattttcg
    17761 ctatcagtca tgatgatcat tactttatcc acgccgaccg cctgctggaa atgcgcaatg
    17821 ggcaacttag cgagctgacg ggcgaagagc gcgatgccgc ttcgcgtgat gccgttgccc
    17881 ggacggcata ata
//
LOCUS       DAEEUE010000081        17697 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.081, whole genome shotgun sequence.
ACCESSION   DAEEUE010000081 DAEEUE010000000
VERSION     DAEEUE010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17697
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.081"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(247..2880)
                     /gene="mngB"
                     /locus_tag="KLK84_002936"
                     /pseudo
     CDS             complement(247..2880)
                     /gene="mngB"
                     /locus_tag="KLK84_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
     gene            complement(2898..4814)
                     /gene="mngA"
                     /locus_tag="KLK84_002937"
     CDS             complement(2898..4814)
                     /gene="mngA"
                     /locus_tag="KLK84_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBE2010185.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            4984..5706
                     /gene="mngR"
                     /locus_tag="KLK84_002938"
     CDS             4984..5706
                     /gene="mngR"
                     /locus_tag="KLK84_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HBE2010186.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(5809..6678)
                     /gene="sucD"
                     /locus_tag="KLK84_002939"
     CDS             complement(5809..6678)
                     /gene="sucD"
                     /locus_tag="KLK84_002939"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HBE2010187.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(6678..7844)
                     /gene="sucC"
                     /locus_tag="KLK84_002940"
     CDS             complement(6678..7844)
                     /gene="sucC"
                     /locus_tag="KLK84_002940"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HBE2010188.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(7938..9155)
                     /gene="odhB"
                     /locus_tag="KLK84_002941"
     CDS             complement(7938..9155)
                     /gene="odhB"
                     /locus_tag="KLK84_002941"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HBE2010189.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(9170..11971)
                     /gene="sucA"
                     /locus_tag="KLK84_002942"
     CDS             complement(9170..11971)
                     /gene="sucA"
                     /locus_tag="KLK84_002942"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HBE2010190.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(12272..12988)
                     /gene="sdhB"
                     /locus_tag="KLK84_002943"
     CDS             complement(12272..12988)
                     /gene="sdhB"
                     /locus_tag="KLK84_002943"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HBE2010191.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(13004..14770)
                     /gene="sdhA"
                     /locus_tag="KLK84_002944"
     CDS             complement(13004..14770)
                     /gene="sdhA"
                     /locus_tag="KLK84_002944"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HBE2010192.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(14770..15117)
                     /gene="sdhD"
                     /locus_tag="KLK84_002945"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(14770..15117)
                     /gene="sdhD"
                     /locus_tag="KLK84_002945"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HBE2010193.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(15111..15515)
                     /gene="sdhC"
                     /locus_tag="KLK84_002946"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(15111..15515)
                     /gene="sdhC"
                     /locus_tag="KLK84_002946"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBE2010194.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            15896..16003
                     /locus_tag="KLK84_002947"
     CDS             15896..16003
                     /locus_tag="KLK84_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010195.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            16209..17492
                     /gene="gltA"
                     /locus_tag="KLK84_002948"
     CDS             16209..17492
                     /gene="gltA"
                     /locus_tag="KLK84_002948"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HBE2010196.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT         4203 a         4794 c         4392 g         4308 t
ORIGIN      
        1 tataattaac tcacccgtgg ttattatttt gttaaaaata aagccacata tacgtagctt
       61 gagagtgaag ataaacacaa agttacctga tgcgctacgc ttgccagtgt ctacatcatc
      121 tctgcaatat attgaatttg cctgcttttg tagaccgaat aaagcgttca tgccgtatcc
      181 ggcatgaaca aagcgccctt tgtcaacaat ctggccgcgc atatgtggct tcggtttgat
      241 tgctattcag gcaagccggt aactgaacgt ccgtgactgg ccgggtaaaa aaggccccga
      301 tagatttgaa ccttgatttt cctcggtggt aatgtgttca tccatcatcg tttctgagca
      361 agaaatcacc tcgcgactga aagcaacagt cgcatcacag gttgctgatt cagccggatt
      421 aaacagccgt aaaatcactt cttgtcggtc ttcagctttc ttaagtgcgc ttatcaggca
      481 tcccactggg ggcattttca acaaactata actttccggc acgttgaatc cggctttgtt
      541 gagcttcatc gcatcccatg ggattttgtt gtaacactgt actggagtca gccatgctcg
      601 cgcctgctga gctacaccag cggcggttgg cgtaccggta taactcaata aacttaggcg
      661 acaagaaagc agaccacgta gttgtgagtc cgggactggc attttaattc ccgaaggccg
      721 cctaggcctt aaaagcagat cttctttccc cagtaagccc acgccgcgca gcaacgtaat
      781 ggcaaaggtt ttcttctctt caccgatgac ttcaaattca cgtagccctt cgctaaagac
      841 agccatgccg ttacgccctt cctgtaaggc tgcatagttg agcatattcc ataccggaac
      901 cggcgcttct ttccagcctt cttgctgcca gttgttcatt gcactgtcgt tcacggggcg
      961 cgttagcgaa ccaaactggg tatctgccag aacactatcg gtgttaaaag atgtagggat
     1021 caggacgcga aggcgatgat cgtcagcctg gttatcaagg ttgatatcca catcaatgcg
     1081 cctgttgtta tgactaagag tgacaaccaa ctcgacatct actctgccag tggattgtct
     1141 tgcgctgcgt tgttgcagat tacgcggcac cgccatttca tagcggataa cagccctgct
     1201 ctgccaggcc tcatgaataa tctcgcattg cggtttcgca ttcgccgaag taattaccca
     1261 ctcttctttt gcgggtgaat agtcatattc atcaccatca tcagagcttt cttcaatttg
     1321 caacacccgg tcatagcgca caccgctatc tttatctacc agttgtagcg ttccatcctc
     1381 attgagcgca atttgccaga aagcattttc cagtatccct tcagcgtcac tttttgccgc
     1441 aatcacgttg ccaggctgat tcgcttcgat ataaagcgtg cgatagccca tagaagggac
     1501 aatctggttg atctgtatat caaactccat aaagggatcg taattaccgt aatgaactat
     1561 ttgccgatcg attaggcctg gatcgatctc acgggcatgg cgaataaaat acggtacagg
     1621 ctgaccgcga tcgtctcgta gattaaactg gctggcgcgc agccgcacag tggtgttgat
     1681 cacttcttcc cgcggccagg gcatcaggtt aaacaggacg agtttgtcgg cgtcgctctg
     1741 cggcatgttg tcggcgattt tacgcatata gaaacgcagc agattatccg ccatgtcttc
     1801 agccagttcg aagcgggcga cgatttcgcg atgaactttg tcactacagc agcagccgat
     1861 gctgtcgtgg gcatgatttt ttaagatctc tttccacatt ttctccagca agccgtggtg
     1921 gtatccaaaa cccaacgtcc tggccagcgt tgccagcggt tccagcagat taacaatctt
     1981 attttcaata cgtgcgtggg caactttgat atccatgcgc gtggaaccga tggtgcgatg
     2041 cacgcgcata tatttgccat caataaattc ccctttcagg gttgccagat tatctcgctg
     2101 cgcttcgatc ttctcaaata cctcttcaaa gcggctcatc acaaactttc gttgaggata
     2161 aatttcgcgc agtttctcca tcacttcgaa gatattttgc tgcaatggca tctgatcatg
     2221 cccattcggc agcaaaatct ctttggttac agacgctttt tccagcacgt caaaataact
     2281 gtcgaggcgt ttgcgtaatc cgttttcgtc cgcaggtaag tacttaccga tggcataacc
     2341 caacggcatc acctgcgccg tcacttcgct accgtcactg ctttgccaca aaaactcggt
     2401 tttatccgta ccgtggtgct cagaacatcc acgccagaac atggtgcagg taatgccaaa
     2461 tccattgtag atatgcggca gttgcccgga catgccaaag gaatcgggta aataacctat
     2521 tttcatcggc tcgccaaacg cgaggcagtc acgcattccg tacatcagat tacggacgat
     2581 ggattccgca gaaacaatcg tggtatcggt ctgggtatac taggggccga taatcaactt
     2641 gccggcttct accagtttct tcacgcggtc tttgttttcc ggtttcaccg cgaaataatc
     2701 ttcgaggatc gccgtttgcc cgtcgagtac gtaatatttg tattcgttgt cctgttccag
     2761 tcggcacagg atctcttcca tattattgac cagcagaatg cgtgactctt cggtggtgaa
     2821 ataccactcc cgatcccagt gcatatgcgg ggtgatgtga acgcgagata ccgctttcat
     2881 cgtcgtttcc tttttgttta tggcattacg ccatcagtca gatagttgcc atgcttaacc
     2941 gcgtgacgcc gccagatcag gagaattgca gtcgagattg cagcccccac caatgccgca
     3001 ccaaaccagc caattgccgc cataacaccg cccgcgccat tatcatgaag taaaaagagc
     3061 gagaaaatgc cagcaccggg tgtcgaaagg ccgatattca tcgcaccgac aatagcgccc
     3121 gttaccatag agcccagcac aaacgaacca ataacccgca gcggatcttc aatcgccatc
     3181 gggatcgccc cttcggtaat acctgccagc cctaacagcc aggtggattt cccggtctca
     3241 atttcaaact ctttaaacag gcgcggtgcg agcatcgtgg aagcggttac ggtaaatgcc
     3301 gaaaccattt tgacggaggc gaaaatggca tacgggccgt aaacgccgtt cgccattgcg
     3361 cccaggcaga atgcataagc ggctttattc actggccctc caaggtcaaa ggaacacata
     3421 aaaccgagaa tggcacccag caacagcgcg ttacttcctg acagaccgtt cagccaggcg
     3481 gtaagcgagt tattgatcca ggcgacaggt tcccccacca caaacagcat cagactgccc
     3541 gctcccaatg taccgagcac cgggtagaga taaaaagtca ggaagccatt gaatttactg
     3601 ctaagacgca agtgattttt catccagcgt atcaagtaac cggctatcaa tccgccaacg
     3661 accgcgccga gaaacccgga gccgatcatg ttggcggcaa gtccagccgc aaagcctggc
     3721 gctaacgccg gtttatctgc cagcgaatag gcggtatagg ctgccagcac cggtaccatc
     3781 aaaattccga gcagcccgcc gcccagtttg cggtacatcc acagccagga gttttcttca
     3841 ttaaacagat cttgtagccc gaagatttgc gacagtaata ccgcgaccgc cagcaccgtc
     3901 ccccccgcga caatcaacgg tacggcaaaa gagatcccgc tcaacagtgc ctgtttcagc
     3961 tccgttttga cgcttttcac cggttgcgta tcttgttgta aggtacgcgt ctcatcgcca
     4021 cgctcgaggg taagcgattg ttggatcaac gcttctgcat ggcgaatcgg ctcggcaaca
     4081 ggcacagaaa gcgcaggaat gccgttaaaa cgctcactct ccttgatggc gacttcagcc
     4141 gcaaaaatac aggcagttgc actattgagt tgatccgccg ttaaacgccc ttcaataccg
     4201 ttagcacctt gcttttcaac gtagacattc acgccgagtt tgcgtccggc tttttccaga
     4261 tactccgcag ccatgtaggt gtgagcaata cccgccgggc aggccgttac gcaaacgata
     4321 gttggcgcgt tggaaaaaga ggcagaaggt tgcgtgcctc ccttgtcatc cagcgccgag
     4381 agcaattcat caggcgtcgt cgccgactgt atacgcgccc gaatctcatc atccgccagg
     4441 cgcgtggtca gcgctgtcag cagttgcatg tgcgtagtac ccgcttcatt gaggggaatc
     4501 gccagcaaca ccactaaatc aaccgcttcc gggccatcca cgccttccca ctgaagcggc
     4561 tcgctgagtg tggcgaccgc aaacgccgct tcttttaccg cagcagtttt gccatgcggc
     4621 acagccaacc cttcacttaa ggcggtcggg ccaaggcttt cacggcgata cacttctttc
     4681 agaaattgct cagtactgga aattttcccc agagcagcaa gacgttgagt caacgcgtga
     4741 atggcctctt cacggctggt aaagcgcgca ttcagacaca acgcatcgcg gtgggtcaga
     4801 gtcgtcaggt tcatcatgat cctcacttga tcgtttttat agtcgcgcca gtgtgcccga
     4861 caaaacaata ccattgcgta atcaatttca cttgattgaa attatttgca ttttatttgt
     4921 attgtttaca ggatgaattt atcgggcata atggctaaat tcgtattaat gagatgctaa
     4981 ataatgggac acaaaccctt ataccgacag attgctgatc gcattcgtga gcaaattgcg
     5041 cgtggcgagc ttaaacctgg cgatgcgtta cctaccgaat cggcattaca aacagagttt
     5101 ggcgtcagcc gggttacggt gcgtcaggct ttgcgccagt tagtcgagca acagatcctc
     5161 gaaagcattc agggcagcgg gacttacgtc aaagaagagc gggtcaatta cgatattttt
     5221 cagttaacca gttttgatga aaaactgtcg gaccgtcacg tcgatacaca cagtgaagtt
     5281 ctgatatttg aagtgattcc ggctgacgat tttcttcagc aacagctaca aatcaccgtg
     5341 caggatcgtg tgtggcatgt gaagcgcgtg cgctatcgca agcaaaagcc aatggcgctg
     5401 gaagaaacct ggatgccgct tgcgttgttc ccggatctca cctggcaggt catggaaaat
     5461 tcgaaatatc actttattga agaagtgaag aagatggtta tcgatcgtag cgaacaggaa
     5521 atcattcctc tgatgccaac cgaagagatg agccgtctgc ttaatatcag ccagacaaag
     5581 ccgattcttg aaaaagtatc gcgtggatat ttagttgatg gtcgggtgtt tgaatatagc
     5641 cgcaacgctt ttaataccga tgactataaa tttaccctga tagcgcagcg aaaatcatcg
     5701 cgataagcac aaaaaggcca tcatacgatg gccaaccatg tcgaaaccga cagcccttcg
     5761 ccattcctgt agagggaagc ggcgagggct atttcttatt acagatattt atttcagaac
     5821 agttttcagt gcttcaccga tatccgccag gctgcgaacg gttttcacgc ctgcggcttc
     5881 cagagcagcg aatttctcat ccgcagtccc tttcccaccg gcaatgatcg cacccgcgtg
     5941 gcccatacgt ttgcctttcg gcgcagtcac accagcgatg taacccacaa ctggcttggt
     6001 aacatgctct ttgatgtacg ctgccgcttc ttcttcagcg ctaccgccga tctcaccgat
     6061 catcacgatc gcttcggtct gcggatcttt ttcgaacatt tcgagaatgt cgataaagtt
     6121 agagccaggg atcgggtcac cgccgatacc gacacaggtc gactgaccga aaccgtaatc
     6181 cgtggtctgt ttaaccgctt cataggtcag tgtaccggaa cgggaaacga tacccacttt
     6241 acccggtttg tgaatgtgac ccggctggat accaattttg cattcacccg gggtgataac
     6301 gcctgggcag ttcgggccga tcatacgaac gcctgcttcg tccagtttca ctttcacggt
     6361 cagcatatcc agcgtcggga tgccttcagt gatggtgata atcagtttga tgcctgcatc
     6421 gatagcttcc agaatggagt ctttgcagaa cggtgctggt acgtaaataa cagacgcgga
     6481 agcgccagta gcagcaacag cttcacgcac ggtgttgaac accggcaggc cgaggtgggt
     6541 agtgccgcct ttacctggag ttacgccacc aaccatttta gtgccgtatg caatggcctg
     6601 ttctgagtgg aaagtcccct ggctaccggt aaagccctgg cagataacct tggtgttttt
     6661 atcaattaaa atggacatta tttcccctcc actgcggcaa caacctgctg agctgcatcc
     6721 gtcagacctt ttgctgcaat aatattcagg ccgctgtcag ccagtttctt cgcgccgagt
     6781 tcggcgttgt taccttccag acgtaccacg accggtacgt taacacccac ttccgctacc
     6841 gcgccgatga taccatcagc gatcaggtcg caacgaacga taccgccgaa gatgttaacc
     6901 agaacggctt tcactttgtc gtcagagagg atgattttga acgcttcggt tacacgttct
     6961 ttggttgcgc cgccgccaac gtccaggaag ttagccggtt cgccgccgtg cagtttaact
     7021 atgtccatcg tacccatcgc cagacctgcg ccgttaacca tacaaccgat gttaccgtcc
     7081 agcgcaacgt agttcagttc ccactgtgca gcctgtgctt cacgcggatc ttcctgcgac
     7141 tggtcacgca tttcgcgcag atcaggctgg cggaacaggg cgttgccatc agcgcccagt
     7201 ttgccgtcga ggcaaatcag atcgccctgt ttggtgatga ccagcgggtt gatttcgatc
     7261 agcgccaggt cgcgctccag gaaaatggtc gccaggccca tgaagatttt ggtgaactgc
     7321 tgaaccagtt taccttccag acccagtttg aacgccagct cgcgtccctg atacggcatc
     7381 gggccagtca gcggatcaag cgcaacttta tggatcaggt gcggagtttc ttccgccact
     7441 ttttcgattt ccacgccgcc ttcggtggag gccataaaga ccacgcgacg ggaactacgg
     7501 tctacaacgg caccgagata cagctcttta gcgatatcgg tcgccgcttc aaccagaatc
     7561 tggttaaccg gttggccatt ggcatctgtt tgatacgtta ccagacgctt gcccagccag
     7621 ttttctgcaa aagcacggat gtcttctttg ctgtttacaa ctttcacacc gcccgcttta
     7681 ccgcggccac cagcgtgaac ctgacatttc actacccacg gaccggcacc gatttttgaa
     7741 gcggcttctt ctgcttcgcg cggagtagta caggcataac ccaccggtgc tggtaagcca
     7801 tagcgggcaa acagttgttt tgcctgatat tcatgtaagt tcatgtgttc tgtccatcct
     7861 tcagtaatcg ttatctttta aaccgtagac cgacacccta tccacgtcgg tctacagtgc
     7921 aggtgaagct taaactacta cacgtccagc agcagacgcg tcggctcttc cagtaactct
     7981 ttgatcgtta ccaggaagcc cacggattcg cgaccatcga tcagacggtg atcgtaggac
     8041 agcgccaggt acatcatcgg caggatctca acctgaccat tcaccgccat cggacgatct
     8101 ttgatagcgt gcatacccag aattgcgctc tgcggcgggt tgatgatcgg cgtagacatc
     8161 agggaaccga acacaccacc gttggtgatg gtgaagttac caccggtcag atcttcaacc
     8221 gtcagcttgc catcacggcc tttgactgcc agctctttga ttttcttctc gatgtctgcc
     8281 atgccgaggg tatcgacatc acgcagaacc ggcgtcacca ggccgcgcgg cgtagaaacc
     8341 gccatgctga cgtcgaaata gttgtggtaa accacgtcat cgccgtcgat agacgcgttc
     8401 acttccgggt aacgtttcag ggcttcaacc accgctttca cgtagaagga cataaagccc
     8461 agacggatgc cgtggcgttt ttcaaacgct tcaccgtact gcttacgcag atccataatc
     8521 ggcttcatgt tgacttcgtt gaacgtggtc agcatggcgg tggagttttt cgcttccagc
     8581 agacgctctg ccacacgctt acgcaggcga gtcatcggga cacgtttttc actacgtgca
     8641 gccagagccg gttgcgccgc cggagcagcc gctgccggag cagactcttt cgccggggct
     8701 ttcgccagat gtttttccac atcttcacga gtcagacgac cacccacacc ggtgccttta
     8761 atggcgctgg cgtcgagatt gtgttcagcc agcaggcgac ggatcgccgg gcttaacgca
     8821 tcgttgtttt gctcttccag agacgcctgc tggcgttgcg ccggagtgga cgctttctct
     8881 tcagatttgg cgctggtttc tttaccggcg ctgttgcctt cacgcaggcg accaaggatc
     8941 tgacgagacg ttaccgttgt accttcatct tccagaaccg catccagaat gccgtctgct
     9001 gatgccggta cttccagtac cactttgtca gtttcgattt ctaccagcac ttcatcacgt
     9061 acgactgcgt cgccgggttt tttatgccag gttgcgacgg tggcatcggc tacggattca
     9121 ggcaggtcag ggaccagaat atctacgcta ctcattgtgt atcctttatt tattcgacgt
     9181 tcagcgcgtc attaaccaga tcttgttgct gtttctggtg aacggacata taccctaccg
     9241 ccggagaggc ggaggccggg cggcctgcat aacgcagaga agccccaaac ggaatcactt
     9301 cacggaaatg atgctggctg cagtaccatg cgccctggtt gagcggctct tcctggcacc
     9361 agacaaaatc cttgacgtga gcaaactgct gcaacacttc ctgcatcgct ttatgcggga
     9421 acgggtagag ttgctcgata cgcacaatgg cgacatcgtg ttgattgttc ttacgacgct
     9481 gttccagcag gtcgtaataa accttaccag aacacatcac tacgcgcttc acgcccttcg
     9541 gatcaagctc gtcgatttca ccgatggctg gcaggaaggt gccgttcgcc agttcttcga
     9601 ggctggaaac cgccagcgga tgacgcagca gggatttcgg cgacatcacg accagcggac
     9661 gacgcatccc gcgcagcgcc tgacgacgca gcatgtggta aacctgtgcc ggggtagacg
     9721 gtacgcaaac ctgcatgttt tgctcagcac aaagttgcag ataacgttcc agacgcgcgg
     9781 aggagtgctc cggcccctgc ccttcgtaac cgtgcggcag caacatcacc agaccacaca
     9841 tccggcccca tttctgctcg ccagaggaga tgaactggtc gataaccacc tgcgcaccgt
     9901 tggcgaagtc accgaactgc gcttcccaga tggtcagggt gcgtggttct gcggtggcat
     9961 aaccatattc aaacgccagc acggcttctt ctgacagtac ggagtcccag acacggaacg
    10021 cgccctgacc gttatggatg tgttgcagcg gcgtgtaagt ggaaccgtta gactggttgt
    10081 ggatcaccgc gtggcggtgg aagaaggtac cgcgaccgga gtcttcaccc gacaggcgaa
    10141 ccggaatgcc ttcatcaacc agcgtggcgt aagcgaggtt ttccgcaccg ccccagtcga
    10201 acagtttctc accggctgcc atcgcctggc gatcgccata aatcttggca acgcgagact
    10261 gcatttcaac tgcttctggt accgtactga tacgtttagc cagttcctgc aggcgcttca
    10321 tctcaacttt gttcgggtac tcttcgtccc attcgtggtt gaggtacggc gaccaggtga
    10381 aagagtgcat gttcatcgga cgccactctg ccactacgca atcgccagca tccagcgcat
    10441 cgcggtacag gttaaccatc tcggtggcat cttccagcgt cgccactttt tcctgctcca
    10501 gcttgtcagc gtagattttg cgcggtgtcg gatgtttttt gattttctga tacatcagtg
    10561 gctgggttgc gctcggctcg tcggcttcgt tgtggccgtg acggcggtag cataccaggt
    10621 cgatgaagac atcacgttta aaggtgttac ggaaatcgag cgccagacgg gtcacaaagg
    10681 caacggcttc cggatcatcc gcgttaacgt ggaaaatcgg tgcctgaacc atcttaccga
    10741 tatcagtaca gtacggtgta gaacgggcat ccagcgggtt agaggtggtg aaaccaacct
    10801 ggttgttgat aacgatacgt accgtaccgc caacttcata accacgcgct ttcgacatgt
    10861 tcagagtttc ctgaaccacg ccctgcccgg tcactgcggc gtcaccgtgg atggtgattg
    10921 gcagcacttt gttgctgttc ggctcatcaa gtctgtccag acgggcacga acagagccga
    10981 taacgaccgg gcttacaatc tcaaggtgag acgggttaaa cgccagcgcc aggtgcacca
    11041 ggccgccatc ggtctggaag tcagacgaga agcccatgtg gtatttcacg tcgcctgtgc
    11101 cgaggtgttc tttatgttta ccggcgaact cgtcgaacaa gtcttgcggt tttttaccca
    11161 gcacgttcac cagcacgttc agacgaccac ggtgcgccat cccgagaacc acttcgcggg
    11221 tgccgctgtt gccagcgtgg cggatcatct ctttaagcat cgggattaac gcgtcaccgc
    11281 cttccagcga gaagcgtttt gcgccaggga attttgcgcc gaggtaacgc tcaaggcctt
    11341 cagcggcggt cagttcgctt aagaagcgtt ttttctcttc gctattgaaa gtcgcgcgac
    11401 cagactcgat acgctgttgg atccagcgtt tttcttcggt gctggtgatg tgcatatact
    11461 cggcaccaat cgggccgcag taggtttgct tgagggcgga aataagctcg ccgagtttca
    11521 tggtttcttt gccgctggca aatgaaccga cgttgaaggt ttcctggaag tctgcttcgg
    11581 tcagatcgtg gaaagacgga tccagatcgg ccactttatc ttgctgccac agtcccagcg
    11641 gatctagatt cgcatgctgg tgaccacgga agcggtatgc gttaatgagc tgcaggactt
    11701 taacctgctt cacattggtg tcagggtcgg agatcgttga agagtaacgt gaagcgtctt
    11761 tcgccaggcg gcggaaatat tcacgcgttt gagagtggaa ttgatccggt ttgactcccg
    11821 taccaggtaa ctgctggaac gtcgaacgcc agttagcgtc aaccgagtca ggatcggtta
    11881 agaagtcttc atagagctgt tctatccagc tctggtttgc gccagagagg taagaagagt
    11941 ccaaccaggc tttcaaagcg ctgttctgca tcgtgatccc ttaagcatct tttttatgct
    12001 tacttcgccg tggatactac cacgcacagt gatgtgcgtg ccggggttca cttgcgagct
    12061 cttgtctacg tagtggctcg cgaaggaacc tttaaaaact gtcttagtgt caggggcagt
    12121 ttttaaagat ttcctgcatt tctttgttac gcctgatgcg ctgcgcttat caggcctgcg
    12181 ggtaatgact tgtaggccgg ataaggcgtt cacgccgcat ccggcactgg ttgcctgatg
    12241 caacgcttgc gcgtcttatc aggcctacag tttacgcatt acgttgcaac aacatcgact
    12301 tgatatggcc gatggcgcgc gtcgggttca gccccttcgg acatacactg acgcagttca
    12361 tgatgctgtg acagcggaat acgctgaatg catcgctcaa accgtcgagg cggctgtcag
    12421 tctcggtatc acggctatcg atcaggaaac gatatgccgc taacaagcct gccgggccga
    12481 taaacttatc gggattccac cagaaagacg gacaagaggt tgaacaacat gcgcagagaa
    12541 tacattcata cagcccgtcg agtttttcgc gctgctctgg catctgtaaa tgctcgcgag
    12601 ctggcggatt ttgtccatta ttcaacaggt aaggcttaat tttctcatat tgcgcataga
    12661 attgtcccat gtctaccacc aaatcgcgga tcaccggtaa acctggcagc gggcgaatca
    12721 caatcttctt gcccggctgg ttgagtgccg aaatcggggt aatacaggcc agaccattct
    12781 tgccgttcat gttcagaccg tcggaaccgc acacaccttc acggcaggag cggcggaacg
    12841 acaggctggg atctttctct ttcagctgga taagcgcatc cagcagcatc atgtcgcgac
    12901 cttcttccgc ttccagggtg taatcctgca tacgcggagc atcatcaaca tccgggttat
    12961 agcgataaat tgaaaactcg agtctcattt tcctgtctcc gcattagtaa gtacgaatct
    13021 tcggcgggaa tgccgggcgc agtttcggtt ccatgttgac gcttcggcgc gtcatggatt
    13081 ccgactcagg cagatacagg gagtggcaca gccagttttc atcatcacga tccgggaagt
    13141 cgaagcggct atgtgcgcca cggctttcgg tacggaagtt ggcagaaaca gccgttgcat
    13201 acgccgtttc catcaggtta tccagttcca ggcactcaac gcgctgggtg ttgaactcgc
    13261 tggaagtgtc atccagacgg gcatttttca gacgctcgcg gatcactttc aactgctcaa
    13321 gccctttcgc catcgcatca ccttcacgga agaccgagaa gttatgctgc atacattctt
    13381 gcagcgcttt acggatcgcc accggatctt caccgttacg attattgttc caccggttca
    13441 ggcgatccag agacgcttca acatcagact cgctggcatc gtgcagtgcg ccctgctcgg
    13501 cgatagactc ttgcagatgc agacctgccg cgcgaccaaa gaccaccagg tccagcagcg
    13561 agttgccgcc cagacggtta gcgccgtgta ccgatacaca agcgatttca ccaacggcaa
    13621 acagtcccgg aacaaccaca tcttcgcctt tctcattcac agtcagcgcc tgaccggtaa
    13681 ctttggtcgg aataccgccc atcatgtagt gacaggttgg gataaccgga atcggctctt
    13741 tcaccggatc gacgtgagcg aaggtacggg aaagctccag gatacccggc agacgggatt
    13801 cgagaacttc tttacccagg tgatcgagtt tcagtttcgc gtgtggcccc cacggaccat
    13861 cacagccgcg accttcacgg atttcaatca tgatggaacg cgccaccaca tcacggcccg
    13921 ccaggtcttt ggcgttcggc gcataacgct ccataaagcg ttcgccatgt ttgttcagca
    13981 gataaccgcc ttcaccacgg caaccttcgg tgaccagtac gcccgcaccg gcaatgccgg
    14041 tcgggtggaa ttgccacatt tccatatcct gcaccggtac gcctgcgcgg atagccatgc
    14101 cgacaccgtc gccagtgtta atgtgggcgt tagtggtgga ctgataaata cgccctgctc
    14161 cgccagtcgc cagcacggta gcgcgggctt tgaaataaac cacttcaccg gtttcgatgc
    14221 acagtgcggt acaacccacc accgcgccat cctggttttt caccagatcc agcgcatacc
    14281 actcggagaa aatggtggtg tggtttttca ggttctgctg ataaagcgtg tgcaacagtg
    14341 cgtgaccggt acggtcagca gccgccgcag tgcgtgccgc ctgctcgccg ccgaagtttt
    14401 tcgactgacc gccaaacgga cgttgataga tacgaccatc atcgagacgc gagaacggca
    14461 ggcccatgtg ttcgagttcc agaatcgctt ccggcccggt tttacacata tattcaatcg
    14521 cgtcctggtc accgatatag tccgaccctt ttacggtgtc gtacatatgc cattcccagt
    14581 tatcttcatg ggtattaccc agcgcaacgg taatgccgcc ttgcgcagaa acggtatggg
    14641 aacgggtcgg gaagacttta gagagcaggg cacaggtctg gccgctctgg gaaatttgca
    14701 gcgccgcgcg catacctgcg ccaccggcac caatcacaac tgcatcaaat tctctgactg
    14761 gcaatttcat cacacacccc acaccacaac gaatccataa atcacgtaaa ccaccagtgc
    14821 aacgacaatc accagttgca gcatcaggcg caaggccagc ggtttaacgt agtcggtcaa
    14881 cacctgccac atgccgatcc aggcatgaat caagatagaa aacagcgcca gcagggtgaa
    14941 cactttggtg aacgcagagg cgaagaaacc gatccagact tcatatgtca gttcgccact
    15001 ggtggcgaaa aaaccgacca tataaatgat gtagagcgtc aggacgatag cggtagcgcg
    15061 aacgaggatg aaatcatgta cgccattgcg tcctaatgcg gaggcgttgc ttaccatacg
    15121 aggactcctg cgagaagtga aagcacgaca gtaataacaa aggagatttt ggcggagcgt
    15181 ttacccgctt cgaatgtttc ttccagatag ccaaaatcca tcatcatgtg gcgaatacct
    15241 acgacgacgt gatacgccag agcggtaagg atgccccaca tgataaattt gacgaagaag
    15301 ctgcccataa tcgcggaagc ttgctcgaaa ccttcagggg aagagaggct ggtacccaga
    15361 agccactgca ggatgcccac tgcaacaaag gtgatcacac cggaaacgcg atggagaatg
    15421 gacgctatcg ccgtgatggg gaaccggatg gtctgtaggt ccagattaac aggtctttgt
    15481 tttttcacat ttcttatcat gaataacgcc cacatgctgt tcttattatt ccctggggac
    15541 tacgggcaca gaggttaact ttctgttacc tggagacgtc gggatttcct tcctccggtc
    15601 tgcttgcggg tcagacagcg tcctttccat aactgcgcgt catgcaaaac actgcttcca
    15661 gatgcgaaaa cgacacgtta caacgctggg tggctcggga ttgcagggtg ttccggagac
    15721 ctggcggcag tataggctgt tcacaaaatc attacaatta acctacatat agtttgtcgg
    15781 gttttatcct gaacagtgat ccaggtcacg ataacaacat ttatttaatt tttaatcatc
    15841 taatttgaca atcattcaac aaagttgtta caaacattac caggaaaagc atataatgcg
    15901 taaaagttat gaagtcggta tttcacctaa gattaactta tgtaacagtg tggaagtatt
    15961 gaccaattca ttcgggacag ttattagtgg tagacaagtt taataattcg gattgctaag
    16021 tacttgattc gccatttatt cgtcatcaat ggattcttta cctgcaagcg cccagagctc
    16081 tgtacccagg ttttcccctc tttcacagag cggcgagcca aataacaaac gggtaaagcc
    16141 aggttgatgt gcgaaggcaa atttaagttc cggcagtctt acgcaataag gcgctaagga
    16201 gaccttaaat ggctgataca aaagcaaaac tcaacctcaa cggggacaca gctgttgaac
    16261 tggatgtgct gaaaggcacg ctgggtcaag atgttattga tatccgtact ctcggttcaa
    16321 aaggtgtgtt cacctttgac ccaggcttca cttcaaccgc atcctgcgaa tctaaaatta
    16381 cttttattga tggtgatgaa ggtattttgc tgcaccgcgg tttcccgatc gatcagctgg
    16441 cgaccgattc taactaccta gaagtttgtt acatcctgct gaatggtgaa aaaccgactc
    16501 aggaacagta tgacgaattt aaaactacgg tgacccgtca taccatgatc cacgagcaga
    16561 ttacccgtct gttccacgct ttccgtcgcg actcacatcc aatggcagtc atgtgtggta
    16621 ttaccggcgc gctggcggcg ttctatcacg actcgctgga tgttaacaat cctcgtcatc
    16681 gtgaaattgc cgcgttccgc ctgctgtcga aaatgccgac catggccgcg atgtgttaca
    16741 agtattccat tggtcagcca tttgtttatc cgcgcaacga tctctcctat gccggtaact
    16801 tcctgaatat gatgttctcc acgccgtgcg aaccgtatga agttaatccg attctggaac
    16861 gtgctatgga ccgtattctg atcctgcacg ctgaccatga acagaacgcc tctacctcca
    16921 ccgtgcgtac cgctggctct tcgggtgcga acccgtttgc ctgtatcgca gcaggtattg
    16981 cttcactgtg gggacctgcg cacggtggtg ctaacgaagc ggcgctgaaa atgctggaag
    17041 aaattagctc cgttaaacac attccggaat ttgttcgtcg tgcgaaagat aaaaatgatt
    17101 ctttccgcct gatgggcttc ggtcaccgcg tgtacaaaaa ttacgacccg cgcgccaccg
    17161 taatgcgtga aacctgccat gaagttctga aagagctggg caccaaagat gacctgctgg
    17221 aagtggctat ggagctggaa aacatcgcgc tgaacgaccc gtactttatc gagaagaaac
    17281 tgtacccgaa cgtcgatttc tactctggta tcatcctgaa agcgatgggt attccgtctt
    17341 ccatgttcac cgtcattttc gcaatggcac gtaccgttgg ctggatcgcc cactggagcg
    17401 aaatgcacag ttacggtatg aagattgccc gtccgcgtca gctgtataca ggatatgaaa
    17461 aacgcgactt taaaagcgat atcaagcgtt aatggttgat tgctaagttg taaatatttt
    17521 aacccgccgt tcaaatggcg ggttaatttt tatatgccta aacacaaaaa attgtaaaaa
    17581 taaaatccac tatcggacat tcatatattc taaaaaaaaa tacatcggta taaattataa
    17641 acaaatagat gatttttaat catctatctt cctctcagtt acatgaataa accacca
//
LOCUS       DAEEUE010000082        17173 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.082, whole genome shotgun sequence.
ACCESSION   DAEEUE010000082 DAEEUE010000000
VERSION     DAEEUE010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17173
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.082"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..185
                     /locus_tag="KLK84_002949"
     CDS             <1..185
                     /locus_tag="KLK84_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HBE2010197.1"
                     /translation="EWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHRV
                     DIGNLHYIDTGAVFGG"
     gene            complement(210..401)
                     /locus_tag="KLK84_002950"
     CDS             complement(210..401)
                     /locus_tag="KLK84_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HBE2010198.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(506..742)
                     /locus_tag="KLK84_002951"
     CDS             complement(506..742)
                     /locus_tag="KLK84_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HBE2010199.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="KLK84_002952"
     CDS             complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="KLK84_002952"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HBE2010200.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASWVKVLHA
                     NISRCGISNVALTHFGGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="KLK84_002953"
                     /pseudo
     CDS             complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="KLK84_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="KLK84_002954"
     CDS             complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="KLK84_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HBE2010201.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            6381..6878
                     /gene="msrC"
                     /locus_tag="KLK84_002955"
     CDS             6381..6878
                     /gene="msrC"
                     /locus_tag="KLK84_002955"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HBE2010202.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6975..7673
                     /gene="proQ"
                     /locus_tag="KLK84_002956"
     CDS             6975..7673
                     /gene="proQ"
                     /locus_tag="KLK84_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HBE2010203.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7693..9741
                     /gene="prc"
                     /locus_tag="KLK84_002957"
     CDS             7693..9741
                     /gene="prc"
                     /locus_tag="KLK84_002957"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HBE2010204.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9933..10814
                     /gene="htpX"
                     /locus_tag="KLK84_002958"
     CDS             9933..10814
                     /gene="htpX"
                     /locus_tag="KLK84_002958"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HBE2010205.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10860..12233)
                     /locus_tag="KLK84_002959"
     CDS             complement(10860..12233)
                     /locus_tag="KLK84_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE2010206.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12410..13201
                     /gene="kdgR"
                     /locus_tag="KLK84_002960"
     CDS             12410..13201
                     /gene="kdgR"
                     /locus_tag="KLK84_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HBE2010207.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13344..13583)
                     /locus_tag="KLK84_002961"
     CDS             complement(13344..13583)
                     /locus_tag="KLK84_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010208.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            13742..13885
                     /gene="mgrB"
                     /locus_tag="KLK84_002962"
     CDS             13742..13885
                     /gene="mgrB"
                     /locus_tag="KLK84_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HBE2010209.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13960..14247
                     /locus_tag="KLK84_002963"
     CDS             13960..14247
                     /locus_tag="KLK84_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HBE2010210.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14826..14969
                     /locus_tag="KLK84_002964"
     CDS             14826..14969
                     /locus_tag="KLK84_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HBE2010211.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14982..15191
                     /gene="cspE"
                     /locus_tag="KLK84_002965"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14982..15191
                     /gene="cspE"
                     /locus_tag="KLK84_002965"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBE2010212.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15357..16166
                     /gene="rlmA"
                     /locus_tag="KLK84_002966"
     CDS             15357..16166
                     /gene="rlmA"
                     /locus_tag="KLK84_002966"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HBE2010213.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16163..16729)
                     /gene="mntP"
                     /locus_tag="KLK84_002967"
     CDS             complement(16163..16729)
                     /gene="mntP"
                     /locus_tag="KLK84_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HBE2010214.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIITTALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4532 a         4156 c         4337 g         4148 t
ORIGIN      
        1 atgaatggca aaaggacgtt gatttgcatc aggtcttgtg gagccgctcg cgattaggtg
       61 aacgcccaaa agggcaggga attacaggtg ctgatcattt ctggtttggt catacaccgt
      121 tgcgacatcg cgtggatatt ggcaacctgc attatattga taccggtgct gtctttgggg
      181 gctaactgac tcttgtgcaa ctgcaataat taaaaatcac catactcctg tgctggtcgc
      241 cagaaaccat ctataaaatc ctcaatcgga aaacaaccgc catggcggat ccgttgatcg
      301 ctcatagaat aaagacactg ctgttccgtg ttgtagacat ccacaacaat atcttcacaa
      361 ccgccatcta ggtagcaaac aaaaagtacc agcgcgaaca tttcatcccc gaagtgtggt
      421 gccgtaccgt taagtttagg agagatttta caacggggga ataaccagga caaataaccc
      481 gccatgccga cgggttcttt ttggatcagg caagacgcat aatccaggca tcagattgtt
      541 gatcgtaacg ggtacggtaa aggacagagt gttcgccatt tagcagggca gggatactgg
      601 tttcagcatc ccaggcatcc agttgcatac ataaatcggg gtcagattta caaggaatgg
      661 ttaacgttcg atcacccggc gattcaccgt gtaattcgcc gtcgtcgatt tcaaaagcgc
      721 ctatgcgcac actggttttc atcttcgtgc tctccattta gctgctgatt gtagtacgac
      781 cagtccgatc gcacaatttt tcagcctagt tgaggccggc gaatgtgcca gtcaaaaagt
      841 gcgctttttt tgtgcgctgt catgcgttac tggtaaaaag tttcccgtcc cgcaccagtt
      901 cacgcgggta gctgtttttc agtcgcgaac caatccgttt ggctaaacca atcggctgat
      961 gctggaaagt aaccaacacg tcatccgcca ctggcgcggc ttgcgggtaa acatcgcgcc
     1021 cgcgatacca ctcctccgct tcctgcggtg tcagttcaaa agcgtttaca ttgtcggggg
     1081 aggcatgggc aataaccgct tcatgctgcc agcgataacc tttgttatgc gtttcggcaa
     1141 gtttaatccc taagcgagaa aatcggactt taccgatcag ggcttcaatg cctaccggga
     1201 acaaccacag ttctttgtcg cgttgccaga gtcgcaggtt tccatcccag tttaagccaa
     1261 cacttgcagc cgcctgacga atttgtccgg cttcgcgatc tttcaccggg ctgaacggga
     1321 aattgcccac tttgtatttc ggggcgggta aggcggggat cgcctgagtt ttacgcagac
     1381 gagcaacgaa gaagccttcg cagtcgtaaa tttgtgggaa aacatgcaaa aaaccttctt
     1441 cggtcagcgc cttgtttgca ccagggaaga gatcgccaag tggtaaaaac tctacttcgt
     1501 cggggtaagt ctctttcagc caccggcaaa cggcttcgtt ttcttcccgg tttaaggtac
     1561 aggttgagta aaccagcgta ccgccaggac gtaatgcatg aaaggcgctg tcaatcagtt
     1621 cccgttgggt agctgcgatt tcctgattgc tttcgggtga ccagtttttt agcgcatcgg
     1681 gatctttacg caccacgcct tcgccggagc agggagcgtc cagcaaaatg gcatcaaaca
     1741 tttctggcac tgccgcacca aacacgcggc caccaaaatg tgtgagcgca acattactga
     1801 tgccacagcg gctgatattg gcatgtaaca cttttaccca actggcggaa aactcattgg
     1861 caaggattgc cccttcgtta ttcatccgcg cggcaatttg cgtcgttttt gagcctggcg
     1921 cagcagcgac atccatcacc cgctgtggtg cattaccgtc agcaaacaag gcggcgacgg
     1981 gcaacattga actggcttcc tgaatataaa acaggccact taaatgctcg gtggtactac
     2041 ccaatggcaa tgcatcttca ttgtcgcgtt caatccagaa accttcttca caccacggaa
     2101 ttggcgtaag cgtccagcca taaggagcgg ttaattgcag gaaatcagca acggagattt
     2161 tcagcgtatt aacgcgaatg ctgcggcgca acgggcgctg acaggcggca agaaaatcat
     2221 caaatgagag cgtcgaaggc atagcttcgc gcatttgtgt cagaaacgcg tccgggaaat
     2281 aaacggtgtg ttgggccacg agcatgtacc actggcaaaa aaacaggcgc gcagtttagc
     2341 ataagcgctc cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagttccc
     2401 cattcacgcc actctttcgg ttcactttcc tgcaacagga agtgtttgcc ttcttgggct
     2461 ttcggtgcaa gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaattgg
     2521 ttaaaggtgc cggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat
     2581 gccaaccaga agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc
     2641 gcaatcatca cgcgatctga caatgtcccc agcgtcattc cggtaaccgt acctacttcc
     2701 agaccacgga acagcacagg cgtaccgatg cctaacgaac cggcttccgg cgcttcaaca
     2761 ataatgctta aaccatctag gtaacgcgaa tcagtaatgg tggcctcttc taattcaaag
     2821 tcgcgacgag gattgccacg gcctggttcg acgttgatat acggctggag gatagtatct
     2881 agatgctcaa cgccagctgc cgagatttgc ggtgtgacca ctgagaagcg cgtaccaccg
     2941 cgagcaaagg tctggacata ttccggatag agcaccgcct ttgcctgtac ttcattgcgt
     3001 gcggtaatca gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc
     3061 atcccgaccg ccagttttcc ggcatcgaaa gcgtgaagcg taatctgcct gccaaccgca
     3121 cgggccgctg tttcggaagc atacagaatt cgcttgtcgc ctttacgctg actggcgctg
     3181 gcaccactga ggttatcgaa gctaatggcc ccctttaatg ctctggagag cggggatgcc
     3241 tgtacggtca ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg
     3301 ctgttgctgg tcaaaaggtt gcgatattcc ggtttaatgt gcagatcgat atcgaacgcg
     3361 ttagctcgcg gacgcacggt aataacttca ccaacttcaa atttacggta cagcactacc
     3421 gatcctgcct gcacatccgg cagcgtcttt gcactcaaac tcacggttgt ggtgggtaaa
     3481 tcgctaaggc tgttctccag cgctttttcc agattggcat acagtggata gctggctttc
     3541 atctcgcctt tatcgcccgg cagaatacgt atcccgccgt taatccattc tgaggcgctg
     3601 gcaccgagaa actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca
     3661 aatttgctat cgccttttac cagttctcga tgctgaggct cgatggcgac ggtaaaggtg
     3721 acgcctttgc tggtgagttt acgatcgata acctggccta cctgcacgcc gtgaagaatg
     3781 agcggctgac ccgcatcaat accgtaactt tccggtgctg tcagggtcag tgtcagaacg
     3841 tccggctctt gcagcagggc tttttcgccc ggcacaacaa cgaactcttt gcgtggctcg
     3901 ccatcgccgg gcaccaactc aaaggttttt ccggtcagca gggcgctgag attagcatcg
     3961 ctaagggata atttcgggtt gcgtaattcg atgcgggtat tttcacgaag cagggatcaa
     4021 cggtcatttc cccagtgact ttaccaccag gatttaaatc cagtttagtc agctgtccga
     4081 cttccagccc ctgatacatt aacggcgtcg agtcggcggt taatcctgct ccactcggca
     4141 gttccagttt tattattact ccacgctggc tgtgggccag atcttcatac agaccaaagg
     4201 tatcttccgc ctcggcaggt ttcgactctt caggtgaatc gaaggcaatc gcaccgttaa
     4261 ccagtgccgc cagactttcc agtttcacct tcgcgccact gatactgacg ttggcatcaa
     4321 cgccggaaac gtttcagaaa cggctacctt ttttcaccag atcggtaaaa cgccgctcga
     4381 tcaggacatc aatcaccacg ccttgcttgt tgggattgat ggcatagtcg tagacttttc
     4441 ccaccgggat cttgcggaaa tagaccaatg aaccgctgtt cagcgaaccg agagcggggg
     4501 cttgcaggtg gatcatcaga tcgccattgt ccagccgata tttcggttgg gtatcgagcg
     4561 cgacaaagtg atcctgctct ttacctttac ccggcatcat gccgatatag ttcccaccga
     4621 cgagggcgtc cagcccggag acacctgcca acgatgcttt tggcgtcatc agccagaact
     4681 gagtctcttc gcgcagcgca tctttcatat cggacttgat gctgaccttg acttcaatct
     4741 tacgaagatc gtcgctgagg ctgatatcct gcactgttcc gacttcaacg ccctgataac
     4801 gaacaggcgt acggcccgga acaataccat ccgccgacat aaagtcgatg gtgacggtat
     4861 taccccggtc ctgataactg tcccaaatca gccaaccggc aatcattagc gcgatgaaag
     4921 gcagcagcca gaaaggtgag atacggcgtt tatttttaat ctgcgcttca gtcgtcgaag
     4981 cgggcgtttc ctgactcatg tgcatcccaa agtaagcggc tgtccagcca ttccacagca
     5041 agaatagtca aaattacggc agcgccaaaa taaaacgcag ccggtcccat agtaaaagcg
     5101 aggatctgat cgcgattaat cagcgacatg gttaaagata tgacaaacag gtcgagcatc
     5161 gaccagcgtc caatccaggt caccatccgc agtaacagaa tgcgtgtgcg taaaccttgc
     5221 tggcatttaa aatgaatgct caacagtaaa gtgaacatga cgatcacttt agtaaacggt
     5281 accagaatac tggcgataaa cacgattccc gcaaccgcaa tgttgctact tgccagcgac
     5341 ataattccgg aaagaatcgt atcttcctgc cggccaccat tcagataaat gatagaaata
     5401 ggcaataggt tggctggtaa caacaaaacg attgacgcta acagcgccgc ccagcatttt
     5461 tgcaaactat gacgacgacg caggcgtagc gggatatggc aacgcgggca gagaccacgc
     5521 tgatcaggat agccggtaaa atggcaccca agacagacac gaagtttctc gtccctacgc
     5581 gtagcggggc gctgcggata atatcgctcc cacagctcct caacattaag atgtgacaac
     5641 gtcaccgtcg ttaaaatcac caacgccaca aaagagaaca agccgacacc cgcctggata
     5701 tgggcataat cctgtacctt tatagaagca acgccaatgc cgaccaggta gatatccagc
     5761 attacccact ctttaagtcg ctcaagcatc agcagtaccg gacgtaaatt cattcccagt
     5821 cggttaccaa accataaata tgctatggag gacaccagaa tgaggggggc accaataacg
     5881 cagaaaaaga ccattgatcc cgttatcgca tcgccctgtt tggtcatttg ccagatgcct
     5941 tgcatgacgt tggcgtcaat acgaatgcct aacagccaga tatgcaacag cggttcgccc
     6001 caggcaaacg gcataagcaa cagcatggtg aaggccattg ccgccaggcg cgttagcgac
     6061 cagtcgcgcc cgtcgcgaat ttttgcctga cagcgcggac aataggcact ttgatgagaa
     6121 tttatctcgg gcaggctaaa cagcatgtca cattgcgggc aacgttggta atcaccacgc
     6181 ggcagttcct cgccgattgc cctcactgtt atcttttttg tcggcgtaat ttgtggtgtg
     6241 ttaagagcca tgacgtgcca aatgtaataa gaattaacat taatcttaac ccatgatggg
     6301 aatgatcttg agcattaacg catttaatgc ttattttaat aggctggcag ttccaggtaa
     6361 gacaaccaag tgattaagta atgaacaaaa cagaatttta cgcggattta aatcgcgact
     6421 ttaacgcgct gatggcggga gaaaccagtt ttctggcaac gcttgcgaac accagtgcgt
     6481 tgttatacga gcgtctcact gacgttaact gggcaggttt ttatttgctt gaggacgata
     6541 cactggtact cggaccattt cagggcaaaa ttgcctgtgt ccggataccc gtcgggcgcg
     6601 gggtgtgcgg cacagcggtt gcccgcaatc aagtgcagcg tatcgaggat gttcacgcgt
     6661 ttgacgggca tattgcctgt gatgcggcga gtaattctga aattgttctg ccgctggtgg
     6721 tgaaaaatca gattattggt gttctcgaca tcgatagtac cgtcttcgct cgctttacag
     6781 acgaggacga gcaaggctta cgtcagcttg tggcacagct tgaaaaagtg cttgcaacga
     6841 cggattacaa aaaattcttt gcgagcgtcg caggataatc aacggataac gtagcattta
     6901 ctggtagcgt cattataatg acgcctgttc atgcctgcgc ttgttggcta cgtccgttgt
     6961 aatcaggaaa tttcatggaa aatcaaccta agttgaatag cagtaaagaa gtaatcgcgt
     7021 ttctggccga acgttttccc cactgtttca gtgcggaagg tgaagcgcgt ccgctgaaaa
     7081 ttggtatttt tcaggatttg gtcgatcgtg ttgccgggga aatgaacctg agcaaaacgc
     7141 aattacgatc cgctttacgt ctctacactt cgagctggcg ttatctttac ggtgttaaac
     7201 ccggcgcaac gcgtgtcgat cttgacggca acccatgcgg tgagctggac gagcaacatg
     7261 tagagcatgc tcgcaagcag cttgaagaag cgaaagcgcg tgttcaggca cagcgtgctg
     7321 aacagcaagc gaaaaaacgc gaagctgccg cagctgctgg tgagaaagaa gacgcaccgc
     7381 gccgcgaacg caagccacgt ccgaccacgc cacgccgcaa agaaggcgct gaacgtaaac
     7441 ctcgtgcgca aaagccggta gagaaagcgc caaaaacagt aaaagcacct cgcgaagaac
     7501 agcacacccc ggtttctgac atttcagctc tgactgtcgg acaagccctg aaggtgaaag
     7561 cgggtcaaaa cgcgatggat gccaccgtat tagaaatcac caaagacggc gtccgcgtcc
     7621 agctgaattc gggtatgtct ttgattgtgc gcgcagaaca cctggtgttc tgaaacggag
     7681 gccgggccag gcatgaacat gttttttagg cttaccgcgt tagctggcct gcttgcaata
     7741 gcaggccaga ccttcgctgt agaagatatc acgcgtgctg atcaaattcc ggtattaaag
     7801 gaagagacgc agcatgcgac ggtaagtgag cgcgtaacgt cgcgcttcac ccgttctcat
     7861 tatcgccagt tcgacctcga tcaggcattt tcggccaaaa tctttgaccg ctacctgaat
     7921 ctgctcgatt acagccacaa tgtgctgctg gcaagcgatg ttgaacagtt cgcgaaaaag
     7981 aaaaccgagt taggcgatga actgcgttca ggcaaactcg acgttttcta cgatctctac
     8041 aatctggcgc aaaagcgccg ttttgaacgt taccagtacg ctttgtcggt actggaaaag
     8101 ccgatggatt tcaccggcaa cgacacttat aaccttgacc gcagcaaagc gccctggccg
     8161 aaaaacgagg ctgagttgaa cgcgcagtgg gacagtaaag tcaaattcga cgagttaagc
     8221 ctgaagctga caggaaaaac ggataaagaa attcgtgaaa ccctgacacg ccgctacaaa
     8281 tttgccattc gtcgtctggc gcaaaccaac agcgaagatg ttttctcgct ggcaatgacg
     8341 gcgtttgcgc gtgaaatcga tccgcatacc aactatcttt ccccgcgtaa taccgaacag
     8401 ttcaacactg aaatgagttt gtcactggaa ggtattggcg cagtgctgca aatggatgat
     8461 gactacaccg ttatcaattc gatggtggca ggtggtccgg cagcgaagag taaagctatc
     8521 agcgttggtg acaaaattgt cggtgttggt caaacaggca agccgatggt tgatgtgatt
     8581 ggctggcgtc ttgatgatgt ggttgcctta attaaagggc cgaagggcag taaagttcgt
     8641 ctggaaattt tacctgctgg taaagggacc aagacccgta ctgtaacgtt gacccgtgaa
     8701 cgtattcgtc tcgaagaccg cgcggttaaa atgtcggtga agaccgtcgg taaagagaaa
     8761 gtcggcgtgc tggatattcc gggcttctat gtgggtttga cagacgatgt caaagttcaa
     8821 ctgcagaaac tggaaaaaca gaatgtcagc agtgtgatca tcgacctgcg tagcaatggc
     8881 ggtggggcgt tgaccgaagc ggtatcgctc tcaggtctgt ttattcctgc gggtcccatt
     8941 gttcaggtcc gcgataacaa cggcaaggtt cgtgaagaca gcgataccga cgggcaggtg
     9001 ttctataaag gcccgctggt ggtactggtt gaccgtttca gtgcttcggc ttcagaaatc
     9061 tttgccgcgg caatgcagga ttacggtcgt gcgctggttg tgggtgaacc gacgtttggt
     9121 aaaggcaccg ttcagcaata ccgttcattg aaccgtattt acgatcagat gttacgtcct
     9181 gaatggccag cgctgggttc tgtgcagtac acgatccaga aattctatcg cgttaacggc
     9241 ggcagtacgc aacgtaaagg cgtaacacca gacatcatca tgccgacggg taatgaagaa
     9301 acggaaacgg gtgagaaatt cgaagataac gcgctgccgt gggatagcat cgatgccgcg
     9361 acttatgtga aatcaggaga tttaactgcc tttgaaccgg agctgctgaa ggaacataat
     9421 gcgcgtatcg cgaaagatcc tgagttccag aacatcatga aggatatcgc gcgcttcaac
     9481 gctatgaagg acaagcgcaa tatcgtttct ctgaactacg ctgtgcgtga gaaagagaat
     9541 aatgaagatg atgcgacgcg tctggcgcgt ttgaacgaac gctttaaacg agaaggtaaa
     9601 ccggagttga agaagctgga tgatctatcg aaagattacc aggagccgga tccttatctg
     9661 gatgagacgg tgaatatcgc actcgatctg gcgaagcttg aaaaagccag acccgcggaa
     9721 caacccgctc ccgtcaagta atatcaatca ggcacaagaa attgtgcctg attttttaac
     9781 agcggcaaga tgccgtaaat cagatgctac aaaatgtaaa gttgtgtctt tctggtgact
     9841 tacgcactat ccagacttga aaatagtcgc gtaacccata cgatgtgggt atcgcatatt
     9901 gcgttttgtt aaactgaggt aaaaagaaaa ttatgatgcg aatcgcgctc ttcctgctaa
     9961 cgaacctggc cgtaatggtc gttttcgggc tggtactgag cctgacaggg atacagtcga
    10021 gcagcgttca ggggctgatg atcatggcct tgctgttcgg ttttggtggt tccttcgttt
    10081 cgcttctgat gtccaaatgg atggcattac gatctgttgg cggggaagtg atcgagcaac
    10141 cgcgtaacga aagggaacgt tggctggtca atactgtagc aacccaggct cgtcaggcgg
    10201 ggatcgctat gccgcaagtg gctatctacc atgcgccgga catcaacgct tttgcaaccg
    10261 gtgcgcgccg tgatgcctct ctggttgctg tcagcaccgg tttgctgcag aacatgagcc
    10321 cggatgaagc cgaggcggta attgctcacg aaatcagcca catcgccaat ggtgatatgg
    10381 tcaccatgac gctgattcag ggcgtggtga acaccttcgt tatctttatt tcccgtattc
    10441 tggcgcagct tgccgcgggt tttatgggcg gaaatcgtga tgaaggtgaa gagagcaacg
    10501 gcaacccgct gatctacttt gcggttgcaa cggttctgga actggtgttt ggtattctgg
    10561 cgagcattat caccatgtgg ttctcgcgtc atcgtgaatt ccatgctgat gccggttcgg
    10621 caaaactggt tggtcgcgag aaaatgattg ccgcgctgca gcgcctgaaa accagctatg
    10681 aaccgcaaga agcaaccagc atgatggctt tctgcattaa cggtaagtcg aaatcgctca
    10741 gtgagttgtt catgactcac ccgccgctgg ataaacgtat tgaagctctg cgtacgggtg
    10801 aatacctgaa gtaatatgaa gtaaatacta aaaagcgcgt cctgatcgac gcgctttttt
    10861 tatgccctgg ctcgtggctg agtgatacgt aaaccactga cacaggcagc aatcactgcc
    10921 agaatagccg cagccatcag cgagacgtgc gtaccattat caccaaactg atttagcatc
    10981 agcgccacca gcgccgcgcc actactctga cccagtagac gagccgttcc taacatgcca
    11041 ctggccccac cgctacgttc gcgcggagcg gaggtaataa tggtgtggtt attgggtgac
    11101 tggaataagc caaatccagc gccacataag atcatcggcc agataatatt gatatccgca
    11161 ggtgacgcgg gcagcagaac cagggaaaaa agccccgtag ccatgatgaa caaccctaaa
    11221 gcacccagca atcctgcatg tacgcgttca atcaaatagc ctgccagcgg agccatcacc
    11281 atcgttgcta acggccacgg tgtcagaagt aaacctgttt cgacttcact acgcccgagc
    11341 acggtttgca ggtaaaaggg cagggaaacc attgccagca tttgtgcgca gaaagagcaa
    11401 acagatgtgc aaatagaaag tgaaaacagc gggatacgca gtaaatccac cggtagcagc
    11461 ggtacgggaa gagaaagctg gcggcgaata aagaaaatac caacaacaac cattaccacc
    11521 agttccgcag caattaacgt cagcgattgc ccctgagcga aaccactcaa cgcagtgata
    11581 agcaggccga aggttaacgc gttcatcacg gcgctgggca ggtcgaaacg gggtttactg
    11641 gcgcgagaac cattgggtgg cagaaaacgc atcgccagaa gcagggcgat aatacctaac
    11701 ggtacgttga ttaaaaataa ccatttccag gatgcgatgg agaggattgc tgcagcaatt
    11761 gtcggcccgg cagcagaaga gacggcaaca ataaacgagt ttatgcccat ccctctcccc
    11821 agaaaacgtt gtggatagat caggcggata agtgcggtat taacgctcat caacgctgca
    11881 ccgccgaaac cttgtatgac acgcgcaagg gtgagcattt gcagcgaatc agaaagggcg
    11941 cagaacagtg aagacaacag aaaaacgacc agaccgcatt tataaatacg tcgatagcca
    12001 aacatatcgc ccagaaacga aaacgagagc agggagatga caatggcgat ttgataggcg
    12061 ttcactaccc agatggaact ggctggtgtg gcatgaaggt ccgtggcaat tgttggcagg
    12121 gcgacgttgg cgattgcgcc gtcaaggaca gccatcgaaa taccaatcac aatggttaat
    12181 atcgcaccgt atcgctgggg caatggcagg ccgtcggcct gaacttttgg cataggaatt
    12241 ttatatcttt ggtgaataat caacaggggt atgattctag cactgatatt cacggtggat
    12301 gtcgcagaat tgtaactaac cggcaaaaga tggattgcga ccaccctggt gcgaaattat
    12361 aataaaaaac agttctgatt tttataaaac actcgcaatg aggtgataaa tggctaacgc
    12421 agatctggat aaacagcctg attctgtatc ttccgtgcta aaagtttttg gcattttgca
    12481 ggcgctgggt gaagagcgcg aaatagggat aaccgagctg tcgcagcgcg tcatgatgtc
    12541 aaaaagcacc gtttatcgct ttttacagac catgaaaacc ttaggttatg tggcgcagga
    12601 aggggagtcg gagaaatatt ccctgaccct gaaattgttt gaactgggcg ctcgcgcgtt
    12661 acaaaacgtc gatttaattc gtagcgcaga tatccagatg cgtgagatct cccgcctgac
    12721 caaagaaact atccacctcg gcgcactgga cgaagacagt attgtttaca ttcacaaaat
    12781 tgactctatg tacaatttgc gcatgtattc acggattggg cgtcgtaatc cgctgtacag
    12841 caccgcgatt ggtaaggtac tgctggcatg gcgcgatcgc gatgaagtga agcaaattct
    12901 tgagggcgtg gagtataaac gcagtaccga gcggaccatc accagtacag aagcgttatt
    12961 acccgttctg gaccaggtgc gcgagcaggg gtatggagaa gataacgaag agcaggaaga
    13021 agggctgcaa tgcattgcgg taccggtatt tgatcgcttt ggcgtggtca ttgccggttt
    13081 gagcatctcc ttcccgacgt tgcgtttctc tgaagagcgt ttacaggaat atgtcgcaat
    13141 gttgcatacc gcagcgcgca aaatttctgc ccaaatgggt tatcacgact atccgttctg
    13201 atgagagtaa gaacctgtcg gaatatcaaa cagacaggtt ctttatttag catgagaaaa
    13261 ataaagttga aggtgcgtta tattaaacgc gcttgctata agaatatttt actcatgagt
    13321 gagaatctgg ttatttatta cccttaacca ttatcgacca cgatattgct tttacgtaac
    13381 agtgggcaat ctgttatctc caagaaacca cttttagtgt gcaagtattg taccgtgctg
    13441 gtgcctctgg cagtcagata ggtacattgc aaacctaatc ctgcggcatt ctctttgctt
    13501 ccaatcaaaa cgccatatcc gctgagtaat aatcctatcc ataccagtgc tatcagcata
    13561 actgtgcgaa tgatgaatcg cattacaatc tcttctcttt ttatgttcgc ttaatcgtag
    13621 cggcaatatg cgctgaagca agctactcat tccgaaaaag cacgaatatc gacatagtta
    13681 ggcgctgttt aactaacgca tgctagttta atgacataag gtaggtgaag cggagattgg
    13741 agtgaaaaag tttcgatggg tcgttctggt tgtcgtggtg ttggcttgct tgctgctttg
    13801 ggcgcaggta ttcaacatga tgtgcgatca ggatgtacaa tttttcagcg gaatttgtgc
    13861 cattaaccag tttatcccgt ggtgatatca ttttttcagc actagatttt cttcctctcc
    13921 agcagtggta gaatagcgcc tctactcaga ggtggtgaaa tgaacgaagt tgtaaattca
    13981 ggcgtgatga acattgcgtc tttggttgta tcggtggtgg ttcttcttat cgggctcatc
    14041 ttgtggtttt ttatcaatcg tgccagttca cggactaacg aacagattga actgcttgag
    14101 gcgttgctgg atcagcaaaa acgtcaaaat gcactgttac gtcgtttgtg cgaagcaaac
    14161 gaaccagaga aagcagataa aaagaccatt gagagtcaaa aatcggttga tgacgaagat
    14221 attattcgcc tggtcgccga acgataatcc ttcctcaatc ttgagtaacc ccgtcgttga
    14281 agggttactc aattcatcct gattatacgc actgtcatgg tgcaactgct tcaaattaaa
    14341 actctggtct gacttcaaat aattatcata ttatttatta gcgaaacgtt tctctcttga
    14401 tttttttgct cactcatcaa tttttcttat tttaaattta caatcccttt ggggattgac
    14461 ttctctttag ggtaattgat agccgttaac tgactgtttt atgagaaaaa gtgatataac
    14521 tttttattca ttgcatagca gaaaatgtga tattgcacgc actatgtaat aacttctccc
    14581 tctggcctgg aacaactgaa cttattgaac tatgttagaa actacgccag tttaagtatc
    14641 tgcctgaact ggcaaggtta agcacaatga tatatcggcg cgtattccgt tgcataagtg
    14701 tgcaaaaaaa gtggaagacg tatcgagatt tgtgcgtctg atcgagacat gtttaaaaat
    14761 ggcttgccat aattaacgtt gtatgtgata aaagatttcg ggttaaacga ggtacagttc
    14821 tgtttatgtg tggcattttc agtaaagaag tcctgagtaa acacgttgac gttgaatacc
    14881 gcttctctgc cgagccttat attggtgcct catgcagtaa tgtgtcagtt ttatctatgt
    14941 tatgcctgcg ggcgaagaaa acaatctaag gaatttttca aatggcaaag attaaaggtc
    15001 aggttaagtg gttcaacgag tctaaaggtt ttggcttcat tactccggct gatggcagca
    15061 aagatgtgtt cgtacacttc tccgctatcc agggtaatgg cttcaaaact ctggctgaag
    15121 gtcagaacgt tgagttcgaa attcaggacg gccagaaagg tccggcagct gttaacgtaa
    15181 cagctatctg atcgaatcca ctgatctgaa gtgtgaatac gcttcaatct cgctataaag
    15241 cctcgtcgaa tgcgaggctt tttactatgc tttatcttcg ctcctggcgt tcggatattt
    15301 gcccgccgcg tgattcgcgt tacacttgcg gcctttagta tcctgctgga gttgtcatgt
    15361 cgttttcctg tccactttgc catcagcctc tttcgcgtga aaaaaacagc tatatctgtc
    15421 cccaacgaca tcagtttgat atggcgaaag aagggtatgt caatctgctg cccgttcagc
    15481 ataaacggtc tcgtgatccg ggcgacagcg cggaaatgat gcaagcacgc cgcgcattct
    15541 tagatgccgg acattatcag ccgctgcgtg atgcaattgt cgcccaactg cgggaacggc
    15601 ttgatgaaaa ggccacggcg gtgctggata ttggctgtgg tgaagggtat tacacacacg
    15661 catttgccga tgcgttgccc gaaatcacca cgtttggcct ggatgtctcg aaggtagcga
    15721 taaaagcggc ggcgaaacgc tatccgcagg tcactctttg tgtcgcttcc agccaccgtt
    15781 tgccgttttc cgataccagt atggacgcca taatacgtat ttacgcgccg tgtaaagcag
    15841 aagaattagc acgagtagtg aagcccggcg gctgggtcat tactgccacg ccgggaccgc
    15901 gacatttgat ggagctgaag gggctgattt acaatgaagt acatcttcat gcacctcatg
    15961 cagaacaact ggaaggtttt acattacagc agagtgcgga gttgtgttat ccgatgcgtc
    16021 ttcgcggtga tgaagccgtc gcattattgc agatgacgcc gtttgcctgg cgtgcgaagc
    16081 cagaagtctg gcaaacactg gcagcaaaag aagtgttcga ctgccagacg gactttaata
    16141 ttcacctctg gcagcgttct tattaaccgt ggaagtgcgt ccagaggatc tggacgccga
    16201 tgccgatcag caccagcccg ccgagaattt ccgctttttt cccaataatt gagccgataa
    16261 agcgaccaac catcatccct aatgtcgaca taatcaaggt tgcacaacca atggccaatg
    16321 cggtcgtgat aatgttgacc tgaaggaaag caagaccaac acccacagcc atggcatcca
    16381 ggctggtggc aatcgcggtg gttaccagta gccagaaacc gtgccgacgg cgcggctctt
    16441 catcttcatc atctgcgcca cgaaaaccct caataatcat tcgcccgccg aggaatatca
    16501 gcagcacaaa cgcaatccag tgattccatt caaggacaaa ccggctggct aacatgccca
    16561 ttccccagcc gatcagcggc gtcagggttt cgacggcacc aaaaataagg ccggttcgca
    16621 atgcttcaga aaatttcggt ttatggaggg gggcaccttt accgattgat gcagcaaatg
    16681 catccatcga cataccaaac gcaagaagaa cagtagcagt gatattcatg acaacgtcct
    16741 gaccggggta tccataaaac acatcatcgc ccccagcaaa catgcataca tgaacaacaa
    16801 gccgcgatgg gttttcgcgt tcatgcaggc atagcgattg atgtgcatat ggtctcgcct
    16861 gactgaaagt cttcagtccg tacgtgccac gttttgcaac gagtatgttg acacgtacat
    16921 ttccggaatc tggaaatcgg ctactcccca aatgagggcg caaccttaac atattttgag
    16981 aatataaaac aactgttgga atatattatt tcattgcttt attgataacg attttcattt
    17041 atgtttatat atcaatgaaa cgttaaaata aataacaaat aagggaagag ataatatagc
    17101 ctaagctatg tctgattatt aaaaatacac taaacatagc tttggctaaa ttcataagtt
    17161 attgagttga tac
//
LOCUS       DAEEUE010000083        17145 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.083, whole genome shotgun sequence.
ACCESSION   DAEEUE010000083 DAEEUE010000000
VERSION     DAEEUE010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17145
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.083"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(52..306)
                     /locus_tag="KLK84_002968"
     CDS             complement(52..306)
                     /locus_tag="KLK84_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HBE2010215.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPVTLFVPLIMGC"
     gene            616..2388
                     /gene="aspS"
                     /locus_tag="KLK84_002969"
     CDS             616..2388
                     /gene="aspS"
                     /locus_tag="KLK84_002969"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBE2010216.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2506..2958
                     /gene="nudB"
                     /locus_tag="KLK84_002970"
     CDS             2506..2958
                     /gene="nudB"
                     /locus_tag="KLK84_002970"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HBE2010217.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2987..3727
                     /locus_tag="KLK84_002971"
     CDS             2987..3727
                     /locus_tag="KLK84_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBE2010218.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3762..4283
                     /gene="ruvC"
                     /locus_tag="KLK84_002972"
     CDS             3762..4283
                     /gene="ruvC"
                     /locus_tag="KLK84_002972"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBE2010219.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4285..4887)
                     /locus_tag="KLK84_002973"
     CDS             complement(4285..4887)
                     /locus_tag="KLK84_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HBE2010220.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4956..5021
                     /gene="yobI"
                     /locus_tag="KLK84_002974"
     CDS             4956..5021
                     /gene="yobI"
                     /locus_tag="KLK84_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HBE2010221.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5160..5771
                     /gene="ruvA"
                     /locus_tag="KLK84_002975"
     CDS             5160..5771
                     /gene="ruvA"
                     /locus_tag="KLK84_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBE2010222.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            5780..6790
                     /gene="ruvB"
                     /locus_tag="KLK84_002976"
     CDS             5780..6790
                     /gene="ruvB"
                     /locus_tag="KLK84_002976"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBE2010223.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6937..7722)
                     /gene="znuB"
                     /locus_tag="KLK84_002977"
     CDS             complement(6937..7722)
                     /gene="znuB"
                     /locus_tag="KLK84_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HBE2010224.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7719..8474)
                     /gene="znuC"
                     /locus_tag="KLK84_002978"
     CDS             complement(7719..8474)
                     /gene="znuC"
                     /locus_tag="KLK84_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HBE2010225.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8553..9485
                     /gene="znuA"
                     /locus_tag="KLK84_002979"
     CDS             8553..9485
                     /gene="znuA"
                     /locus_tag="KLK84_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HBE2010226.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9501..10823
                     /gene="mepM"
                     /locus_tag="KLK84_002980"
     CDS             9501..10823
                     /gene="mepM"
                     /locus_tag="KLK84_002980"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HBE2010227.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10943..11914
                     /gene="lpxM"
                     /locus_tag="KLK84_002981"
                     /gene_synonym="msbB"
     CDS             10943..11914
                     /gene="lpxM"
                     /locus_tag="KLK84_002981"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HBE2010228.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(12045..13487)
                     /gene="pyk"
                     /locus_tag="KLK84_002982"
     CDS             complement(12045..13487)
                     /gene="pyk"
                     /locus_tag="KLK84_002982"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBE2010229.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKDY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13615..14484)
                     /gene="hexR"
                     /locus_tag="KLK84_002983"
                     /gene_synonym="yebK"
     CDS             complement(13615..14484)
                     /gene="hexR"
                     /locus_tag="KLK84_002983"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HBE2010230.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14821..16296
                     /gene="zwf"
                     /locus_tag="KLK84_002984"
     CDS             14821..16296
                     /gene="zwf"
                     /locus_tag="KLK84_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBE2010231.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16531..>17145
                     /locus_tag="KLK84_002985"
     CDS             16531..>17145
                     /locus_tag="KLK84_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBE2010232.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPVMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRM"
BASE COUNT         4375 a         4227 c         4464 g         4079 t
ORIGIN      
        1 ccccccggat catggacgac tgggggcagt aatcatgccg ggtctggctg gctaacaccc
       61 cattataagg ggtacgaata aagtaacggg ggcatcaacc ggctgtttta atgcttcggc
      121 gtaatcggca gaccagccaa ctcgcaccag aaacacgggc tgaccgctgg cgcgaaattt
      181 cgccgccagc ttcccggcgc gattaactac ctcatcggca gtatgtggac caccggcaaa
      241 aggtaagatg ccttcttgta aatcgatcac caccaacgca gtggttttag cattaagttc
      301 aagcatgctg actcctgaaa aaacaaaatc gtgcctcaca ccttaatgcg attagccgat
      361 ggcggaacga caaattttgt taatttttat gagttcgggc aacaccttta tagcaaactg
      421 gcgtagagtc tgatttcagg cccgatataa agtggtaacg aggcgttcca gcgcgggcca
      481 ggctcttacg gtgcggcaga atttccagta taatagccgc cttttttcat tcagttgtga
      541 catacagcta acgctgcgac ttggtcacct gcgcagcagg gggcatccgc cggcagctaa
      601 gttaagggat atctcatgcg tacagaatat tgtggacagc tccgtttgtc ccacgtgggg
      661 cagcaggtga ctctgtgtgg ttgggtcaac cgtcgtcgtg atcttggtag cctgatcttc
      721 atcgatatgc gcgaccgcga aggtatcgtg caggtatttt tcgatccgga tcgtgcggac
      781 gcgttaaagc tggcctctga actgcgtaat gagttctgca ttcaggttac gggcaccgta
      841 cgtgcgcgtg acgaaaaaaa tattaaccgc gatatggcga ccggcgaaat tgaagtgctg
      901 gcgtcctcgc tgactatcat caaccgcgca gatgttctgc cgcttgactc taaccacgtc
      961 aacaccgaag aagcgcgtct gaaataccgc tacctcgacc tgcgtcgccc ggaaatggcg
     1021 cagcgcctga aaacccgcgc taaaatcacc agcctggtgc gccgttttat ggatgaccac
     1081 ggcttcctcg acatcgaaac tccgatgctg accaaagcca cgccggaagg cgcgcgtgac
     1141 tacctggtgc cttctcgtgt gcacaaaggt aaattctacg cactgccgca atccccacag
     1201 ttgttcaaac agctgctgat gatgtccggt tttgaccgtt actatcagat cgttaaatgc
     1261 ttccgtgacg aagacctgcg cgctgatcgt cagcctgaat ttactcagat cgatgtggaa
     1321 acttctttca tgaccgcgcc gcaagtgcgt gaagtgatgg aagcgctggt gcgtcatttg
     1381 tggctggaag tgaagggcgt ggatctgggc gatttcccgg taatgacctt tgcggaagca
     1441 gaacgccgtt atggttctga taaaccggat ctgcgtaacc cgatggaact gaccgacgtt
     1501 gccgatctgc tgaaatctgt tgagtttgca gtatttgcgg ggccggcgaa cgatccgaaa
     1561 ggccgtgttg ccgctctacg tgttccgggt ggcgcatcgc tgacccgtaa gcagatcgac
     1621 gaatacggta acttcgttaa aatctacggc gcgaaaggtc tggcttacat caaagttaac
     1681 gaacgcgcga aaggtctgga aggtatcaac agcccggtag cgaagttcct taatgcagaa
     1741 atcatcgaag ccatcctgga tcgtactgcc gcgcaagatg gcgatatgat tttcttcggt
     1801 gccgacaaca agaaaattgt tgccgacggg atgggcgcac tgctcctgaa agtgggtaaa
     1861 gaccttggtc tgaccgacga aagcaaatgg gcaccgctgt gggttatcga cttcccgatg
     1921 tttgaagacg acggtgaagg cggcctgacg gcaatgcacc atccgttcac ctcaccgaaa
     1981 gatatgactg ctgcagaact gaaagctgca ccggaaaatg cggtggcgaa cgcttacgat
     2041 atggtcatca atggttacga agtgggcggt ggttcagtac gtatccataa tggtgatatg
     2101 cagcagacgg tgtttggtat tctgggtatc aacgaagagg aacagcgcga gaaattcggc
     2161 ttcctgctcg acgccctgaa atacggtact ccgccgcacg caggtctggc attcggtctt
     2221 gaccgtctga ccatgctgct gactggcacc gacaatatcc gtgacgttat cgccttcccg
     2281 aaaaccacgg cggcagcgtg tctgatgact gaagcaccga gctttgctaa cccgactgca
     2341 ctggctgagc tgagcattca ggttgtgaag aaggctgaga ataactgata tgactcaaat
     2401 acacgaaatc attcgcgttg catcaaggcg gcaactgagt gaactcccat gagcatagat
     2461 aactatgtga atgggatgag cgaaggcagt caacgaagag gcagcgtgaa ggataaagtg
     2521 tataagcgtc ccgtttcgat cttagtggtc atctatgcac aagatacgaa acgggtgctg
     2581 atgttgcagc ggcgtgacga tcccgatttc tggcagtcgg taaccggcag cgtggaagag
     2641 ggtgaaaccg cgccgcaagc tgccatgcgc gaagtaaagg aagaggttac cattgatgtt
     2701 gtcgctgaac aactgacctt aattgactgt cagcgcacgg tagagtttga aattttttca
     2761 catttacgtc atcgctatgc gccgggcgtg acgcgtaata cggaatcatg gttctgtctt
     2821 gcgcttccgc acgagcggca gatcgttttc actgaacatc tggcttacaa gtggcttgat
     2881 gcgcctgctg cggcggcgct cactaagtcc tggagcaacc ggcaggcgat tgaacagttt
     2941 gtaattaacg ctgcctgaac aggcagggct atctggagat atttttatgg caggtcatag
     3001 taaatgggcc aacaccagac atcgtaaagc tgcgcaggat gctaagcgcg gtaaaatctt
     3061 cactaaaatc attcgtgagc tggtaaccgc ggctaagctg ggcggtggcg atccggacgc
     3121 taacccgcgt ctgcgtgcgg cggtggataa agcactgtct aacaacatga cccgtgacac
     3181 actgaaccgc gcaattgcac gtggtgtggg cggtgatgat gatgcaaaca tggaaaccat
     3241 catctacgaa ggttacggtc ctggcggcac ggcaatcatg attgaatgtc tgtctgacaa
     3301 ccgcaaccgt accgttgctg aagtgcgtca cgcattcagc aaatgtggcg gtaacctcgg
     3361 cactgacggt tccgtagctt atctgttcag caaaaaaggc gtgatctcct tcgagaaagg
     3421 cgacgaagac accatcatgg aagcagctct ggaagcaggt gctgaagacg ttgtgacata
     3481 tgatgacggc gcgattgatg tctacactgc atgggaagaa atgggtaaag tgcgcgacgc
     3541 tctggaagcg gcaggtctga aagcagacag cgcggaagtt tccatgatcc cgtccaccaa
     3601 agctgatatg gatgcagaaa ccgcgccgaa actgatgcgt ctgatcgata tgctggaaga
     3661 ttgcgacgac gtgcaggaag tttaccataa cggtgaaatc tctgatgagg ttgcagcgac
     3721 tctctgatga tgcctgctaa acagcaaaac ggagacgcgt gatggctatt attctcggca
     3781 ttgacccggg ttcgcgcgtg accggctacg gcgtcatccg ccaggtaggt aggcaactgt
     3841 cctacctggg tagcggatgc atacgcacca aagtggatga tttaccgtct cgtctgaagc
     3901 tcatctatgc gggcgtgacg gaaatcatca cccagttcca gcctgattat ttcgccattg
     3961 aacaagtctt tatggcgaag aacgctgact cagccctgaa actgggccag gcgcgcggcg
     4021 tggcgattgt tgcggcggtg aatcaggaat tgccggtatt tgaatacgcg gcacgtcagg
     4081 taaagcaaac ggtggtaggt atgggtagtg ccgaaaaaag ccaggtgcag catatggtcc
     4141 gcaccttgct gaaactgccc gctaatccac aggcggatgc cgccgatgcg ctggcgattg
     4201 ccatcaccca ctgccacgtt agtcagaatg cgatgcagat gagcgaatcg cggctgaacc
     4261 tgacgagagg gcgactgcgt taagttatac cgcctcggtc agttccggct gaggcgtttc
     4321 aactccctcc gcgtgaatca acaccaaacc cggatgatgc cacaaactgg ctggcgtgac
     4381 ggttttacgc tcccacggaa tagaacctaa gaaccagaat ttccagaaag tgagttttgc
     4441 attcggattg ctatttaaca gttgtccaaa cgtctctatt tcacccaccg gaatacatag
     4501 cgcctcttta taaatatcaa agacaaattt tgaacagaac tggcgcgaag attcgtattt
     4561 aaaacccgtg tggtaaagtt tgcgtagccg ggagggaacc tgttcaacaa ttcgttgttt
     4621 ttgttgttct gtcagcccag cgtctaatcg ctttatagca tagcgttgat tagacgaacg
     4681 tttaataaaa cgagatagcg tggtgatggt tgaaagggga acccggcttt ctgcaaccag
     4741 gaagtcttca ccgttatgac cgataatgat cccgacgtga ttactccagc aatttgatgc
     4801 agctgatatt tgaccaaata aggcagcacc tatacatgta aagacgatat ctccaatttc
     4861 atattcagca gggtaattaa tattcacgtt gtcattccat tgaaatagat acgaatatac
     4921 gtaatagcat aaaaaattat ttttcaggag tcgcaatgta tatttttatt acgcatttct
     4981 tcactgaata tgtaatattc aaatatttgc ttccaatata acctgttgaa taaattatac
     5041 tgtgccattt ttcagatcat cgggacacct cgcaagtttt cttcatcctt cgctggatat
     5101 ctatccagca tttttttatc atacagcatt atctttgatt cattacgcag gagcgtcatg
     5161 tgataggcag actcagaggc atcatcattg aaaaacaacc cccgctggtg ttaattgaag
     5221 tgggcggcgt aggctatgaa gtgcatatgc cgatgacctg tttttatgaa ctccctgaag
     5281 cgggtcagga agcgatcgtt ttcacccact ttgtggtgcg tgaagacgcg caactgctgt
     5341 acggttttaa caataaacaa gaacgcacgt tgttcaaaga gttgatcaaa accaacggcg
     5401 tcggcccgaa gttggccctg gcgatcctct ccggaatgtc agcgcagcag ttcgttaatg
     5461 ccgttgagcg tgaagaagtg ggggcactgg tgaaactgcc gggtattggc aaaaaaaccg
     5521 ccgaacgctt gattgttgaa atgaaagacc gatttaaagg tttgcatggc gatctcttta
     5581 cgccagccgc cgacctggta ctcacgtctc ctgctagccc ggcgaccgac gatgctgaac
     5641 aagaagcagt tgccgcgctg gtggcgctag gctataaacc acaagaagca agccgcatgg
     5701 tgagcaaaat cgctcgccct gacaccagca gtgaaacatt aattcgcgaa gccctacgcg
     5761 ccgcgttatg aggtaaagga tgattgaagc agaccgtctg atttctgccg gtaccacttt
     5821 gccggaagat gtagcagatc gcgccattcg ccctaaatta ctggaagagt atgttggtca
     5881 gccgcaggtt cgttcacaga tggagatttt catcaaagca gcgaaactgc gcggcgatgc
     5941 cctcgatcat ttgttgattt ttggtcctcc ggggttgggt aaaactacgc ttgccaacat
     6001 tgtcgccaat gaaatgggcg ttaatttacg cacgacttct ggtccggtgc tggaaaaggc
     6061 gggcgatttg gctgcgatgc tcactaacct tgaaccgcat gacgtgctgt ttattgatga
     6121 gatccaccgt ttatcgccag ttgttgaaga agtgctgtac ccggcaatgg aagactacca
     6181 actggatatc atgattggtg aaggtccggc ggcacgctcc attaaaattg atttgccgcc
     6241 gtttaccctg attggcgcaa ccacgcgcgc aggttcgctg acatcaccgt tgcgcgaccg
     6301 ttttggtatt gtgcaacgtc tggagtttta tcaggtgccg gatctgcaat atatcgtcag
     6361 tcgcagtgca cgctttatgg ggcttgagat gagtgatgac ggtgcgcagg aagtagctcg
     6421 tcgcgctcgc ggtacgccac gtattgccaa ccgtctgctg cgtcgagtgc gtgatttcgc
     6481 cgaagtgaag cacgatggca ccatctcggc agatatcgct gctcaggcgc tggatatgtt
     6541 gaatgtcgat gctgaaggtt tcgattatat ggaccgcaaa ttgttgctgg cggtaatcga
     6601 taagttcttt ggtgggccgg taggtctgga taacctggcg gcagccattg gcgaagaacg
     6661 tgaaaccatt gaagatgtac tggaacctta tttgattcag caaggctttt tgcagcgtac
     6721 accgcgcggg cgtatggcga cgacgcgggc gtggaatcac tttggcataa cgccgccaga
     6781 aatgccgtaa gtcggattgg tcgctcgcgc cgcatccgac actggcagtg tgtctgatgc
     6841 gacactgttc gcgtcttatc aggccagcca gagatagcga accgtaggtc ggataaggcg
     6901 ctcgcgccgc atccgacaaa tgtgttcagc gatagattag ctggcctgct ttttcatcat
     6961 actgataata aataacagtg ccgcacatag caccaccgac ggacccgccg gcgtatcgta
     7021 aaacgcggaa aaggttaaac cgccagttac tgccaccatc cccaccaaaa cagcgacacc
     7081 agccatctgt tccggcgtac gggcaaagcg acgcgcagta gctgcaggaa taatcagcaa
     7141 cgaagtaata atcaacgcac cgacaaattt catcgctaca ccaatcgtca atgccgtcac
     7201 cagcatcaac aacaatttca cttgctgtaa tttcacacca tcaacaaacg ccagatccgg
     7261 gctaatcgtc atcgacagca aattgcgcca ttgccagaac aaaatagcca ccacgatgac
     7321 cacgccaatc gcaatagaga tgagatcttc tggcgtcact gccagtaaat caccgaacag
     7381 gtaagccatc aaatcaacac gaatgttaga catcagacta acgaccacta ggcccagcga
     7441 cagggcactg tgcgccataa tccctaataa cgtgtcgatc gccagctgtg gacgcttctc
     7501 cagccatacc agaccgcccg ccagcagcag cgtaacggca atcaccgcat agaatggatt
     7561 cacgtccagc aacaaaccaa acgcgacacc aagtaatgag gcatgagcca gcgtatcacc
     7621 gaaataagac atacgacgcc agactacaaa cgaacccagc ggacccgcgg cacaggcgag
     7681 catgatcccg gctaaccaac cgggaaataa taattcaatc atgagcgatc atttccccga
     7741 cgcaaaacaa ttcgtccctg taaatcgtga cgatgattat gatgatggcg atagataccc
     7801 agttgttcag caccacgagg accaaacatt gaaataaact ccggatgcag ggaaacaact
     7861 tccggtgtgc cggaacaaca aatgtggtga ttcaggcaca gcacttcatc ggtttttgcc
     7921 atcaccagat gcagatcgtg agaaaccatt aaaacgccac aatccagttc acgacgcaac
     7981 tggtcaataa ggtcatataa cgccacttgg ccatttacat ccacgccttg agtgggttca
     8041 tccagcacta ataattgcgg gcaatttaac aatgctcgcg ctaatagtac acgctgcgtt
     8101 tcgccaccag agagcttttg catcggtgcg ttaatcaggt gcccggcctg gacacgtttc
     8161 agtgcaggca aaatatcttc tttatgtgta ccagggcgta agcgtaaaaa acggtttacg
     8221 gtcagtggca acgtggtgtc gagatacagc ttctgcggta catagccgat gcgcagtttt
     8281 ccgttgcgct tgataacccc ttcatcgggt gttaccagcc cgagcactac ccgtaccagt
     8341 gtcgacttac ctgcgccatt tggcccaagt aaagtcaaaa tttttccagg tttaagttcc
     8401 agcgacacat cagagaggac gcggcgttgg ccaaaagaaa ccgagacatt ttccagggaa
     8461 accagacttg tcatgttaat tttagtcttg cagtagtcat gaaatgttat aatatcacac
     8521 ttctcatatt cattacgatg attggtcgca ttatgttaca taaaaaaacg cttcttttcg
     8581 cagcattatc cgccgctctc tggggaggtg caacacaggc cgcagatgcc gccgttgtcg
     8641 cttcgcttaa acccgttggg ttcatcgctt ctgccattgc tgatggtgta acagaaacgg
     8701 aggttttact gcctgacggc gcttcagaac atgattattc actgcgtcca tcggatgtaa
     8761 aacgcttaca gaacgcggac ttagtcgttt gggttggccc ggagatggaa gcgtttatgc
     8821 aaaaaccggt aagcaaatta ccagaagcga agcaggtaac gattgcgcag cttgaagatg
     8881 tgaaaccgct actgatgaaa agtattcacg gcgatgatga tgatcacgac cacgcggaaa
     8941 aaagtgacga agatcaccat cacggcgatt tcaacatgca tctttggctt tccccagaga
     9001 tagcgcgggc tacagcggtt gcaatccatg gaaaattagt ggaacttatg ccgcaaagtc
     9061 gagccaaact tgacgccaac ctgaaggatt ttgaggcaca attggcctca accgaaacgc
     9121 aggttggtaa cgagctcgcg ccgctcaagg ggaaaggtta tttcgttttt cacgatgctt
     9181 acggctattt cgaaaaacag ttcggactga caccgcttgg tcattttacc gttaaccctg
     9241 agattcaacc tggcgcgcag cgtttacatg aaataagaac acagttggtt gagcaaaaag
     9301 caacctgcgt ttttgctgag ccacagttca ggccagcggt cgttgagagc gtcgcacgag
     9361 ggacatccgt tcgtatggga acgttggatc ccctggggac gaatatcaaa ctgggtaaaa
     9421 caagctattc agaattcctg agtcaattag ccaaccagta tgcgagctgc ctgaaaggag
     9481 attaatgagg aagtgattac gtgcaacaga tagcccgctc tgtcgccttg gcgtttaata
     9541 atttaccgcg accacaccgc gttatgttgg ggtcgctcac cgttcttact ctggccgtcg
     9601 ctgtctggcg gccctatgtt tatcaccgtg atgccacgcc aattgtcaaa accattgagc
     9661 tggaacagaa cgaaattcgt tcgctcttac ctgaagccag tgagccgatt gatcaagctg
     9721 cacaagaaga tgaagccatt ccccaggacg aactggatga caaaatcgcc ggtgaagcgg
     9781 gcgtgcatga atatgttgtt tccactggcg atacgctaag cagcattctc aatcagtatg
     9841 gtattgatat gggtgatatc acccaacttg ctgctgccga caaagaattg cgtaacctga
     9901 aaatcggtca acaactctcc tggacattaa ccgcggacgg cgaactgcag cgcctcacct
     9961 gggaagtgtc tcgtcgtgaa acccgaacct atgaccgtac tgccgctaac ggttttaaaa
    10021 tgaccagcga aatgcagcaa ggagagtggg ttaacaatct gctgaaaggt accgtcggag
    10081 ggagctttgt tgccagcgcc agaaacgccg gtttaaccag cgccgaagtg agcgcagtga
    10141 ttaaagccat gcagtggcaa atggatttcc gcaaactgaa aaaaggcgat gaatttgcgg
    10201 tattaatgtc tcgagaaatg cttgatggta aacgtgagca aagccagctg ctgggcgtac
    10261 gtttgcgttc agaaggtaaa gattattacg caatccgcgc tgaggatggc aaattctacg
    10321 atcgtaacgg tactggtctg gcgaaaggat tcttgcgatt cccgacggcg aaacagttcc
    10381 gtatctcgtc taactttaac ccgcgtcgaa ctaatccggt gacaggtcgc gttgcaccac
    10441 acagaggtgt tgatttcgcc atgccacaag gtacgccagt gctttcagtg ggtgacggtg
    10501 aagtggtggt tgccaaacgc agtggcgcag caggttatta tgtggctatt cgtcatggtc
    10561 gcagctacac cacgcgttat atgcacttgc gcaagattct ggtgaaaccg ggacagaagg
    10621 tgaaacgtgg cgaccgtatc gcgctttccg gtaataccgg acgttcaact gggccgcatc
    10681 tgcactatga agtatggata aaccagcagg ccgtaaaccc gctgacggca aaactgccgc
    10741 gtaccgaagg gctgaccggc tccgatcgtc gcgaattcct ggcgcaggcc aaagagattg
    10801 tgccgcagct acggtttgat taattaacat ccattcgcag ccggtacgca gtcagtaccg
    10861 gcttttttta tttggtgcgg ggcaagttgt gccgctacac tatcaccaga ttgatttttg
    10921 ccttatccga aactggaaaa gcatggaaac gaaaaaaaat aatagcgaat acattcctga
    10981 gtttgataaa tcctttcgcc acccgcgcta ctggggagca tggctgggtg tagcagcgat
    11041 ggcgggtatc gctttaacgc cgccaaagtt ccgtgatccc attctggcac ggctgggacg
    11101 ttttgccgga cgactgggaa aaagctcacg ccgtcgtgcg ttaatcaatc tgtcgctctg
    11161 ctttccagaa cgtagtgaag ctgaacgcga agcgattgtt gatgagatgt ttgccaccgc
    11221 gccgcaagcg atggcaatga tggctgagtt ggcaatacgc gggccggaga aaattcagcc
    11281 gcgcgttggc tggcaagggc tggagatcat cgaagagatg cggcgtaata acgagaaagt
    11341 tatctttctg gtgccgcacg gttgggccgt cgatattcct gccatgctga tggcctcgca
    11401 agggcagaaa atggcagcga tgttccataa tcagggcaac ccggtttttg attatgtctg
    11461 gaacacggtg cgtcgtcgct ttggcggtcg tctgcatgcg agaaatgacg gtattaaacc
    11521 attcatccag tcggtacgtc aggggtactg gggatattat ttacccgatc aggatcatgg
    11581 cccagagcac agcgaatttg tggatttctt tgccacctat aaagcgacgt tgcccgcgat
    11641 tggtcgtttg atgaaagtat gccgtgcgcg cgttgtaccg ctgtttccga tttatgatgg
    11701 caagacgcat cgtctgacga ttcaggtgcg cccaccgatg gatgatctgt tagaggcgga
    11761 tgatcatacg attgagcggc ggatgaatga agaagtcgag atttttgttg gtccgcgacc
    11821 agaacaatac acctggatac taaaattgct gaaaactcgc aaaccgggcg aaatccagcc
    11881 gtataagcgc aaagatcttt atcccatcaa ataaaaaagc ctctcgcgag gagaggcctt
    11941 cgcctgatga taagttcaag tttgcttcag aatattcgaa atctgttgaa ctatcattga
    12001 actataggcc ggatgtggcg ttttcgccgc atccggcaac gtacttactc taccgttaaa
    12061 atacgcgtgg tattagtaga acccacggta ctcatcacgt cgccttgggt gacaatcacc
    12121 aggtcaccag acatcaagta atctttatcg cgcagcagat taaccgcttc gctggcagct
    12181 gctacgccgt cattagcgct atcaaagtgc accggcgtaa cgccacgata gagagcagtc
    12241 aggttcagcg tacgttcatg gcgcgacatg gcgaaaattg gtagaccaga gctgatacgg
    12301 gaggtcatca gcgcggtacg acccgattcg gtcatggtaa tgatcgccgt aacgcccttc
    12361 aggtggttag ctgcgtacat tgccgacatg gcaatagctt cttccacatt gtcgaactga
    12421 acgtccagac ggtgtttaga aacgttgatg ctcgggattt tttccgcacc caggcaaacg
    12481 cgcgccatgg ctgcaacagt ttctgacgga tactgcccag cggcagtttc tgcagacagc
    12541 atcacagcgt cagtaccatc cagaacggcg tttgctacgt ccatgacttc tgcacgcgtc
    12601 ggcatcgggt tagtaatcat tgactccatc atctgggtcg ccgtgattac cgctcggttt
    12661 agctgacgcg cacgacggat caacgctttc tgaatgccga ccagttccgg gtcgccaatt
    12721 tccacaccga ggtcgccacg tgcaaccatt accacgtcag aggcgaggat gatgtcatcc
    12781 attgcatcct ggctgcaaac ggcttccgca cgttcaacct tggcaacaat tttcgcatca
    12841 catcctgcat cgcgtgccag gcgacgggca tagttcagat cttcgccaca gcgtgggaag
    12901 gagacagcca ggtaatctac gccaatcaac gccgcagtct taatgtctgc tttgtctttt
    12961 tcggtcagcg cttcagccga caaaccgccg ccaagtttgt tgataccttt attgttggag
    13021 aggggaccac cgacggtgac ttcggtgaac actttcatgc cctgaacttc cagtactttt
    13081 aactggacgc gaccatcgtc cagcagcagg atgtcaccag gcacgacgtc agcaggcagg
    13141 cctttgtagt cgataccgac tttttctttg tcgccttcac ctttacccag gttggcgtcg
    13201 agcaggaatt tatccccaat attgaggaaa actttgcctt ctttaaaggt ggatacacgg
    13261 attttgggcc cctggaggtc acccagaata gccacatgac gccccagttt tgcggcaatc
    13321 tcacgaactt tatccgcgcg cattttgtga tcttcaggcg agccgtgaga aaagttcata
    13381 cgtacaacgt tggcacccgc cgcgataact ttttcaagat tattatcgcg atctgttgct
    13441 gggcctaacg tggtaacgat ttttgttctg cgaagccttt tggacatgta atactccgtt
    13501 gactgaaaca accaggtgtt gcttgaacat gaaatccgaa tgaaataacg ccgcgatggc
    13561 gataaattaa tctcattcgg atgtcgtata agattaggac agtgacagtc gcttttagcg
    13621 atcgtcactt aaattaagta actgcttatc aaaacgcgat tccttcagcg cttccttgac
    13681 ccgcttcaag ttatctctga attttgcacc gcgtcgcaaa gtaaatcctg tcgccagcac
    13741 atctatcacg gtcagctgtg caagtcgaga aaccatgggc atataaatgt cagtatcttc
    13801 cggtacgtcg agggtaattg ccagcgttgc ttcccgggcg agcggggtac ccgcagaggt
    13861 gagggcaatc accatggcgt cgttttcgcg tgccagctgc gccagctcga ccagattttt
    13921 tgttcttcct gtgtgagaaa tcagcaccac cacgtctccg tcgctacaat tcatacaact
    13981 catgcgttgc agcacgatat catcggagta caccaccgga acattaaaac gaaagaactt
    14041 attcatcgca tcgtgggcaa cggcggcaga agagcctaat ccgaaaaagg cgattttttt
    14101 tgcctgagtg agcaagtcga cggcgcggtt gatggcagat ttatccagtg aatgacggac
    14161 atgatcgagc gttgccattg cggactcaaa tattttcccc gtgtatgatt caacgctgtc
    14221 atcttcattg acgttgcgat taacataggg agtgccattc gccagacttt gtgccagatg
    14281 aagtttaaaa tcaggaaaac cgcgcgtgtc catgctgcga cagaaacgat tcaccgtcgg
    14341 ttcgctaaca ttggcttcca gcgccagagc agcaatactc gaatggatcg cgttatcggg
    14401 cgaagccaga atgacctcgg caactttgcg ctctgatttg ctcaaatgtt ccagctgaga
    14461 ctggattttt tccagcatat tcatgatgta aagagactca cgggtaatga cgatttccgc
    14521 actgaaagaa atcgaaatgc agttttgtca gatattacgc ctgtgtgccg tgttaatgac
    14581 aaaagcagat aaaaaagttg ttattttttt cataacatga tcagtgtcag atttttaccg
    14641 aatggaaaac gatgattttt ttatcagttt tgccgcactt tgcgcgcttt tcccgtaatc
    14701 gcacgggtgg ataagcgttt acagttttcg caagctcgta aaagcagtac agtgcaccgt
    14761 aagaaaatta caagtatacc ctggcttaag taccgggtta gttaacttaa ggagaatgac
    14821 atggcggtaa cgcaaacagc ccaggcctgt gacctggtca ttttcggcgc gaaaggcgac
    14881 cttgcgcgtc gtaaattgct gccttccctg tatcaactgg aaaaagtcgg tcagctcaac
    14941 ccggacaccc ggattatcgg cgtagggcgt gctgactggg ataaagcggc atataccaaa
    15001 gttgtccgcg aggcgctcga aactttcatg aaagaaacca ttgatgaagg tttatgggac
    15061 accctgagtg cacgtctgga tttttgtaat ctcgatgtca atgacactgc tgcattcaac
    15121 cgtctcggcg cgatgctgga tcaaaaaaat cgtatcacta ttaactactt tgccatgccg
    15181 cccagcactt ttggcgcaat ttgcaaaggg cttggcgagg caaaactgaa tgctaaaccg
    15241 gcacgcgtag tcatggagaa accgctgggg acgtcgctgg cgacctcgca ggaaatcaat
    15301 gatcaggttg gcgaatactt cgaggagtgc caggtttacc gtatcgacca ctatcttggt
    15361 aaagaaacgg tgctgaacct gttggcgctg cgttttgcta actccctgtt tgtgaataac
    15421 tgggacaatc gcaccattga tcatgttgag attaccgtgg cagaagaagt ggggatcgaa
    15481 gggcgctggg gctattttga taaagccggt cagatgcgtg acatgattca gaaccacctg
    15541 ctacaaattc tctgcatgat tgcgatgtct ccgccatctg acctgagcgc agacagcatc
    15601 cgcgatgaaa aagtgaaagt actgaagtcc ctgcgccgca tcgaccgctc caacgtacgc
    15661 gaaaaaaccg tacgcgggca atatactgcg ggcttcgccc agggcaaaaa agtgccggga
    15721 tatctggaag aagagggcgc gaacaaaagc agcaatacag aaaccttcgt ggcgatccgc
    15781 gtcgacattg ataactggcg ctgggccggt gtgccattct acctgcgtac tggtaaacgt
    15841 ctgccgacca aatgttctga agtcgtggtc tatttcaaaa cacctgaact gaatctgttt
    15901 aaagagtcgt ggcaggatct gccgcagaat aaactgacta tccgtctgca acctgatgaa
    15961 ggcgtggata tccaggtact gaataaagtt cctggccttg accacaaaca taacctgcaa
    16021 atcaccaagc tggatctgag ctattcagaa acctttaatc agacgcatct ggcggatgcc
    16081 tatgaacgtt tgctgctgga aaccatgcgt ggtattcagg cactgtttgt acgtcgcgac
    16141 gaagtggaag aagcctggaa atgggtagac tccattactg aggcgtgggc gatggacaat
    16201 gatgcgccga aaccgtatca ggccggaacc tggggacccg ttgcctcggt ggcgatgatt
    16261 acccgtgatg gtcgttcctg gaatgagttt gagtaatatc agcgcttatc ctttatggtt
    16321 attttaccgg taacatgatc ttgcacagat tgtagaacaa tttttacact ttcaggcctc
    16381 gtgcggattc acccacgagg ctttttttat tacactgact gaaacgtttt tgccctatga
    16441 gctccggtta caggcgtttc actcataaat cctctgaatg aaacgcgttg tgaatcatcc
    16501 tgctctgaca actcaatttc aggagccttt atgaatccac aattgttacg cgtaacaaat
    16561 cgaatcattg aacgttcgcg cgagactcgc tctgcttatc tcgcccggat agaacaagcg
    16621 aaaacgtcga ccgttcatcg ttcgcagttg gcatgcggta acctggcaca cggtttcgct
    16681 gcctgccagc cagaagacaa agcctctttg aaaagcatgt tgcgtaacaa tatcgccatc
    16741 atcacctcct ataacgacat gctctccgcg caccagcctt atgaacacta tccagaaatc
    16801 attcgtaaag ccctgcatga agcgaatgcg gttggtcagg ttgcgggcgg tgttccggtg
    16861 atgtgtgatg gtgtcaccca ggggcaggat ggaatggaat tgtcgctgct aagccgcgaa
    16921 gtgatagcga tgtctgcggc ggtggggctg tcccataaca tgtttgatgg tgctctgttc
    16981 ctcggtgtgt gcgacaagat tgtcccgggt ctgacgatgg cagccctgtc gtttggtcat
    17041 ttgcctgcgg tgtttgtgcc gtctggaccg atggcaagcg gtttgccaaa taaagaaaaa
    17101 gtgcgtattc gccagcttta tgccgaaggt aaagtggacc gcatg
//
LOCUS       DAEEUE010000084        16386 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.084, whole genome shotgun sequence.
ACCESSION   DAEEUE010000084 DAEEUE010000000
VERSION     DAEEUE010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16386
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.084"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(85..2061)
                     /gene="speA"
                     /locus_tag="KLK84_002986"
     CDS             complement(85..2061)
                     /gene="speA"
                     /locus_tag="KLK84_002986"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HBE2010233.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2070..2201)
                     /gene="yqgB"
                     /locus_tag="KLK84_002987"
     CDS             complement(2070..2201)
                     /gene="yqgB"
                     /locus_tag="KLK84_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HBE2010234.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2337..2552
                     /locus_tag="KLK84_002988"
     CDS             2337..2552
                     /locus_tag="KLK84_002988"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010235.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2856..4010
                     /gene="metK"
                     /locus_tag="KLK84_002989"
     CDS             2856..4010
                     /gene="metK"
                     /locus_tag="KLK84_002989"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBE2010236.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4447..5841
                     /gene="galP"
                     /locus_tag="KLK84_002990"
     CDS             4447..5841
                     /gene="galP"
                     /locus_tag="KLK84_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HBE2010237.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5918..6415
                     /locus_tag="KLK84_002991"
     CDS             5918..6415
                     /locus_tag="KLK84_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HBE2010238.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6510..7217
                     /gene="endA"
                     /locus_tag="KLK84_002992"
     CDS             6510..7217
                     /gene="endA"
                     /locus_tag="KLK84_002992"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HBE2010239.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7297..8028
                     /gene="rsmE"
                     /locus_tag="KLK84_002993"
     CDS             7297..8028
                     /gene="rsmE"
                     /locus_tag="KLK84_002993"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBE2010240.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8041..8991
                     /gene="gshB"
                     /locus_tag="KLK84_002994"
     CDS             8041..8991
                     /gene="gshB"
                     /locus_tag="KLK84_002994"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HBE2010241.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9100..9663
                     /locus_tag="KLK84_002995"
     CDS             9100..9663
                     /locus_tag="KLK84_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HBE2010242.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIAGRWREAAKLIGVDILTMPGVAGHA"
     gene            9663..10079
                     /gene="ruvX"
                     /locus_tag="KLK84_002996"
     CDS             9663..10079
                     /gene="ruvX"
                     /locus_tag="KLK84_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBE2010243.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10255..11235)
                     /locus_tag="KLK84_002997"
     CDS             complement(10255..11235)
                     /locus_tag="KLK84_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBE2010244.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            11253..11957
                     /gene="yggS"
                     /locus_tag="KLK84_002998"
     CDS             11253..11957
                     /gene="yggS"
                     /locus_tag="KLK84_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HBE2010245.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11975..12541
                     /gene="yggT"
                     /locus_tag="KLK84_002999"
     CDS             11975..12541
                     /gene="yggT"
                     /locus_tag="KLK84_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HBE2010246.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12538..12828
                     /locus_tag="KLK84_003000"
     CDS             12538..12828
                     /locus_tag="KLK84_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HBE2010247.1"
                     /translation="MSAVTVNDDGLVLQLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12836..13429
                     /locus_tag="KLK84_003001"
     CDS             12836..13429
                     /locus_tag="KLK84_003001"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HBE2010248.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13422..14558
                     /gene="hemW"
                     /locus_tag="KLK84_003002"
     CDS             13422..14558
                     /gene="hemW"
                     /locus_tag="KLK84_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HBE2010249.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14872..15835
                     /locus_tag="KLK84_003003"
                     /pseudo
     CDS             14872..15835
                     /locus_tag="KLK84_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15880..16383
                     /locus_tag="KLK84_003004"
     CDS             15880..16383
                     /locus_tag="KLK84_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBE2010250.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
BASE COUNT         4057 a         4220 c         4126 g         3983 t
ORIGIN      
        1 cagctagatt atttttaaga atatataaaa agaaagcggg ccgatttacg taaataaatc
       61 gacccgccag cggaataatc ttaattattc atcttcaaga taagtataac cgtacaaacc
      121 tgcctcgaac tcttcaagga actgttgttg cagttcagca tcaagatcgg ttttcttcac
      181 ttgatcgcgg aactgggtta acagcgtttt cggatcgagc tgtacatatt gcagcatgtc
      241 cgccacggta tcgccttcgt cagacagttc tacttctacg ctaccgtcag ggaacacgaa
      301 cacgtcaact gcttcggtat caccgaacag gttgtgcata ttgccaagga tctcctgata
      361 tgcaccgacc ataaagaaac cgagcatcgg cggattctct ggatcgtact ccggcattgg
      421 cattgtcgtg gcaataccat caccatcaat atagtggtcg atagcaccgt cagagtcaca
      481 ggtaatatcc agcagcacag cgcgacgttc cggcacttga tccagccctt ccagcggtag
      541 aaccgggaac aactggtcga tcccccatgc gtccggcatt gactggaaca gcgagaaatt
      601 gacgtacatt ttgtccgcca tacgttcctg cagttcgtcg attatcggac gatgcgcacg
      661 gttttgcgga tccagttgct tctgcacttc atggcacatg ctcagataga gttgttctgc
      721 ccatgcacgt tcttgcaggc taaaggtgcc ggaagagtag ccgatatgaa tgtcgtgcag
      781 atccatctgg ctgtcgtgta accattcacg caaagaacgg cgagttcccg gttcgtgcat
      841 ttcctgccag gtttcccaca tgctttgcag cgcacgcggc gcatcttcta cgggcgcggt
      901 cggcaccgta tattcgttac gttccacgcc gatgatattc gacaccagca cggtatggtg
      961 cgcagtcacc gcacggcccg attcggtgat taccgtcgga tgcggcagac cattttcttc
     1021 acacgcatcg ccaatcgccc agatgatgtt gttggcgtat tcattgaggc catagtttac
     1081 ggagcagtca gactgcgaac gggtgccttc ataatccacg cccagaccgc cgccgacgtc
     1141 gaagcactga atattgacgc ccagcttgtg cagttccaca tagaaacgcg cggattcacg
     1201 aacgcctgtc gcgatatcgc gaatattcgc catctgcgaa ccgaggtgga agtgcagtag
     1261 ttgcaggctg tcgagacgcc cggcttcacg cagggtttca accagttgca gtacctgagt
     1321 cgcagccagg ccgaacttcg atttttcccc gccggaggac tgccatttac ccgagccctg
     1381 cgaagccaga cgtgcacgca cgcccagacg aggaacgaca ttcagacgtt ctgcttcatc
     1441 cagcacaatg gcgatttctg acatcttctc aatgaccaga tagaccttgt gccccatctt
     1501 ctcgccaatt aatgccaagc ggatatattc gcggtcttta taaccgttgc agacgatgac
     1561 gctacgagtc atgccagcat gtgccagcac tgccatcaac tcggctttgg aaccggcttc
     1621 cagacccagc ggttcacccg aatgaatcag ggactcgatc acgcggcggt gctggttaac
     1681 tttgatcgga taaacaagga agtaatcgcc gttatagccg taggattccc tcgcacgttt
     1741 gaacgcggcg ttaatggaac gcaaacggtg ctgcaggatc tgtgggaaac agaacagtgc
     1801 aggcagacgc tgaccctgtg cttcacgagt tttcactaac tgcgcgagat cgacgcgagc
     1861 ttccgggacg tccgggtccg ggcacacgct gatgtggccc agctcgttaa cgtcatagta
     1921 gttattgccc caccaggcaa tattgtaagt acgcagcatc ttgctggctt cctgggagct
     1981 cattgcaacc tcctgcatgg agcgtagtac accgtgttcg cccgctgacg aaggcaaacc
     2041 catagacatg tcgtcagaca tagcgaacct caaattattt tattaagtgt aaaacagtta
     2101 acgactgtcg cagcctggaa ctgcgataac aacccataag cacatggatt ttccagcagt
     2161 gtatgctgac gctccgactg cgcaaccggt ttctttttca taacattatt aagcacataa
     2221 ccgaacgtaa gtgtgacagt tcggcgaaac cacgagaaaa ctcttgtttt tacaagagcg
     2281 cccttgttca gtcctcagta actgtaacca gctcttgaat cctgagaagc gccgagatgg
     2341 gtataacatc ggcaggtatg caaagcagag atgcagagtg cggggaacga gtcttcacca
     2401 gaacggtgag acaggttaag cagcagacaa cggttcatta tttcgtatca cctccacggc
     2461 cgcctgttaa gacgaaccca caagccaaaa ctctgatttc aacccggctg gaagtggcaa
     2521 cacgaaagaa acgtcgtgtg ctttttattt aagccgcgcg ccgcgtttta taccccacaa
     2581 tggcagaaaa ttgcaaaaga taaatacgca gaatgccggc attgtcagga aaaatttcca
     2641 gccacgtttt taacagaatg agacacgatt caaaaaaaag tggaaatcgg gtgaagaatt
     2701 gacctaaaat agccatccag atgttaatcc atccataccg attaacactc agactgccag
     2761 tgtttttaac ctgcagagtc gtggtaggat ccgctaccac agaaaatcca cacaacagtt
     2821 tgagctaacc aaattctctt taggtgatat taaatatggc aaaacacctt tttacgtccg
     2881 agtccgtctc tgaagggcat cctgacaaaa ttgctgacca aatctctgat gccgttttag
     2941 acgcgatcct cgaacaggat ccgaaagcac gcgttgcttg cgaaacctac gtaaaaaccg
     3001 gcatggtttt agttggcggc gaaatcacca ccagcgcctg ggtagacatc gaagagatca
     3061 cccgtaacac cgttcgcgaa attggctatg tgcattccga catgggcttt gacgctaact
     3121 cctgtgcggt tctgagcgct atcggcaaac agtctcctga catcaaccag ggcgttgacc
     3181 gtgccgatcc gctggaacag ggcgcgggtg accagggtct gatgtttggc tacgcaacta
     3241 atgaaaccga cgtgctgatg ccagcaccta tcacctatgc acaccgtctg gtacagcgtc
     3301 aggctgaagt gcgtaaaaac ggcactctgc cgtggctgcg cccggacgcg aaaagccagg
     3361 tgactttcca gtatgacgac ggcaaaatcg ttggtattga tgctgtcgtg ctttccactc
     3421 agcactctga agagatcgac cagaaatcgc tgcaagaagc ggtaatggaa gagatcatca
     3481 agccaattct gcccgctgaa tggctgactt ctgccaccaa attcttcatc aacccgaccg
     3541 gtcgtttcgt tatcggtggc ccaatgggtg actgcggtct gactggccgt aaaattatcg
     3601 ttgataccta cggcggcatg gcgcgtcacg gtggcggtgc attctctggt aaagatccat
     3661 caaaagtgga ccgttccgca gcctacgcag cacgttatgt cgcgaaaaac atcgttgctg
     3721 ctggcctggc cgatcgttgt gaaattcagg tttcctacgc aatcggcgtg gctgaaccga
     3781 cctccatcat ggtagaaact ttcggtactg agaaagtgcc ttctgaacaa ctgactctgc
     3841 tggtacgtga gttcttcgac ctgcgcccat acggactgat tcagatgctg gatctgctgc
     3901 acccgatcta caaagaaacc gcagcatacg gtcactttgg tcgtgaacat ttcccgtggg
     3961 aaaaaaccga caaagcgcag ctgctgcgcg atgctgccgg tctgaagtaa tctttcttca
     4021 cctgcgttca aaggccagcc tcgcgctggc ctttttcttt tacctcaccg cggtggatag
     4081 gcgttcacgc cgcatccggc aaaaaaactg cccgcacaat aacatcattc ttcctgatca
     4141 cgtttcacgg caaattacca tcacaactga aaccgattac accaaccaca ccagcaaaat
     4201 atttgtaata ttttcatatt attattcagc tttcacagtt gttacatttc ttttcagtaa
     4261 agtcttaatt gcagatgaca gcgtttaatc tatgatgata taactcaatt attttcatgc
     4321 ccttaaatca taactaagat aaatgttagt gtaagcgatt acactgatgt gatttgcttc
     4381 acatcttttt acgtcgtact cacctatctt aattcacaat aaaaaataac tatattggag
     4441 ggcatcatgc ctgacgctaa aaaacagggg cggtcaaaca aggcaatgac gtttttcgtc
     4501 tgcttccttg ccgctctggc gggattactc tttggcctgg atatcggtgt aattgctggc
     4561 gcactgccgt ttattgcaga tgaattccag ataacttcgc acacgcaaga atgggtcgta
     4621 agctccatga tgttcggtgc ggcagtcggt gcggtgggca tcggctggct ctcctttaaa
     4681 ctcgggcgca aaaagagcct gatgatcggc gcaatcctgt ttgttgccgg ttcactgttc
     4741 tctgcggctg cgccaaacgt tgaagtactg attctttcgc gcgttctgct ggggctggcg
     4801 gtgggtgtgg cctcttatac cgcaccactg tacctgtcag aaattgcgcc ggaaaaaatt
     4861 cgcggcagta tgatctcgat gtatcagttg atgatcacta tcgggatcct cggtgcttat
     4921 ctttctgata ccgccttcag ctacaccggt gcatggcgct ggatgctggg tgtgattatc
     4981 atcccggcaa ttttgctgct gattggtgtc ttcttcctgc cagacagccc acgttggttt
     5041 gccgccaaac gccgttttgt tgatgccgaa cgcgtgctgc tacgcctgcg tgacaccagc
     5101 gcggaagcga aacgcgaact ggatgaaatc cgtgaaagtt tgcaggttaa acagagtggc
     5161 tgggcgctgt ttaaagagaa tagcaacttc cgccgcgcgg tgttccttgg cgtactgtta
     5221 caggtaatgc agcaattcac cgggatgaac gtcatcatgt attacgcgcc gaaaatcttc
     5281 gaactggcgg gttataccaa caccaccgag caaatgtggg ggacagtgat tgtcggcctg
     5341 accaacgtac ttgccacctt tatcgcaatc ggccttgttg accgctgggg acgtaaacca
     5401 acgctaacgc tgggcttcct ggtgatggct gctggcatgg gcgtactcgg tacaatgatg
     5461 catatcggta tccactctcc gtcggcgcag tatttcgcca tcgccatgct gctgatgttt
     5521 attgtcggtt ttgccatgag tgccggtccg ctgatttggg tactgtgctc cgaaattcag
     5581 ccgctgaaag gccgcgattt tggcatcacc tgctccaccg ccaccaactg gattgccaac
     5641 atgatcgttg gcgcaacgtt cctgaccatg ctcaacacgc tgggtaacgc caacaccttc
     5701 tgggtgtatg cggctctgaa cgtactgttt atcctgctga cattgtggct ggtaccggaa
     5761 accaaacacg tttcgctgga acatattgaa cgtaatctga tgaaaggtcg taaactgcgc
     5821 gaaatcggcg ctcacgatta atctcctcag gcttcctccc gtcgcggagg aagccgcctc
     5881 ttgcagtcat cttttcttcg ctctatcctc tgccgctatg aaaacatccc gtctccctat
     5941 cgccatccaa caggccgtta tgcgccgcct gcgggaaaaa ctcgcccagg ccaacctgaa
     6001 gctagggcgt aactacccgg agccaaaact ctcttacacc cagcgcggaa cctccgccgg
     6061 aacagcctgg ctggaaagct atgaaatccg cctcaatccc gttttgctgt tggaaaacag
     6121 tgaagctttt attgaagaag tggtaccgca cgaactggca catttgctgg tatggaaaca
     6181 tttcggccgc gtagcgccac atggcaaaga gtggaagtgg atgatggaaa gcgtgctggg
     6241 cgttcccgcc cgtcgtacgc atcagttcga actgcaatcc gtgcgtcgca acaccttccc
     6301 ctaccgctgt aagtgccagg aacatcagct taccgtacgc cgccataatc gcgtagttcg
     6361 tggcgaggcc atctatcgct gtgttcactg cggtgaacag ctggttgcga aataaccacc
     6421 tgaactatca ggaactttcc tgatctggct gattgcatac caaaacaact ttcgctacgt
     6481 tgctggctcg ttttaacacg gagtaagtga tgtaccgtta tttgtctatt gctgcggtgg
     6541 tactgagcgc agcattttcc ggcccgacgt tggccgaagg tatcaatagt ttttctcagg
     6601 cgaaagccgc ggcggtaaaa gtccacgctg atgcgcccgg tacgttttat tgcggatgta
     6661 aaattaactg gcagggcaaa aaaggcgttg ttgatctgca atcgtgcggc tatcaggtgc
     6721 gcaaaaatga aaaccgcgcc agccgcgtag agtgggaaca cgtcgttccc gcctggcaat
     6781 tcggtcacca gcgccagtgc tggcaggacg gtggacgtaa aaactgcgct aaagatccgg
     6841 tctatcgcaa gatggaaagc gatatgcata acctgcagcc gtcagtcggc gaggtgaatg
     6901 gcgatcgcgg caattttatg tacagccagt ggaatggcgg cgaaggccag tacggtcaat
     6961 gcgccatgaa ggtcgatttc aaagaaaaag ctgccgaacc accagcgcgt gcacgcggtg
     7021 ccattgcgcg cacctacttc tatatgcgcg accaatacaa cctgacactc tctcgccagc
     7081 aaacgcagct gttcaacgca tggaacaaga tgtatccggt taccgactgg gagtgcgagc
     7141 gcgatgaacg catcgcgaag gtgcagggca atcataaccc gtatgtgcaa cgcgcttgcc
     7201 aggcgcgaaa gagctaacct acactagcgg gattcttttt gttaacccct accccacgcg
     7261 tacaaccgcg tggggagacg acgcggattt ttaactatgc gtatcccccg catttatcat
     7321 cctgaaccac tgaccagcca ttctcacatc gcgctttgcg aagatgccgc caaccatatc
     7381 gggcgcgtac tgcgcatggg gccggggcag gcgttgcaat tgtttgacgg tagcaaccag
     7441 gtctttgacg ccgaaattac cagcgccagc aaaaaaagcg tggaagtgaa ggtgctggaa
     7501 ggccagatcg acgatcgcga atctccgctg catattcacc tcggtcaggt gatgtcgcgt
     7561 ggtgaaaaaa tggaatttac tatccagaaa tcgatcgaac tcggtgtaag cctcattacg
     7621 ccactttttt ctgagcgctg cggcgttaaa ctggatagtg aacgtctgaa caagaagctt
     7681 cagcagtggc agaagattgc aattgctgcc tgtgagcagt gtggtcgtaa ccgggtgccg
     7741 gaaatccgtc cagcgatgga tctggaagcc tggtgtgcag agcaggatga aggactgaaa
     7801 ctgaatcttc acccgcgcgc cagtaacagc atcaatacgt tgcctttacc ggttgaacgc
     7861 gtccgcctgc tgattggccc ggaaggcggt ttatcggcag atgaaattgc catgactgcc
     7921 cgctatcaat ttactgatat cctgttggga cctcgcgttt tgcgtacaga gacaactgcg
     7981 ctcaccgcca ttaccgcgct acaagtacga tttggcgatt tgggctaacg gagaagaata
     8041 atgatcaagc tcggcatcgt gatggacccc atcgcaaaca tcaacatcaa gaaagattcc
     8101 agtttcgcta tgttgctgga agcacagcgt cgtggttacg aacttcacta tatggagatg
     8161 ggcgatctgt atctgatcaa tggcgaagcc cgcgcccata cccgcacgct gaacgtgaag
     8221 cagaactacg aagagtggtt ttcgttcgtc ggtgaacagg atctgccgct ggccgatctc
     8281 gatgtgatcc tgatgcgtaa agacccgccg tttgataccg agtttatcta cgcgacctat
     8341 attctggaac gtgccgaaga gaaagggacg ctgatcgtta acaagccgca gagcctgcgc
     8401 gactgtaacg agaaactgtt taccgcctgg ttctctgact taacgccaga aacgctggtt
     8461 acgcgcaata aagcgcagct aaaagcgttc tgggagaaac acagcgacat cattcttaag
     8521 ccgctggacg gtatgggcgg cgcgtcgatt ttccgcgtga aagaaggcga tccaaacctc
     8581 ggcgtgattg ccgaaaccct gactgagcat ggcacttgct actgcatggc gcaaaattac
     8641 ctgccagcca ttaaagatgg cgacaaacgc gtgctggtgg tggatggcga gccggtaccg
     8701 tactgcctgg cgcgtattcc gcaggggggc gaaacccgtg gcaatctggc tgccggtggt
     8761 cgcggtgaac ctcgtccgct gacggaaagt gactggaaaa tcgcccgtca gatcgggccg
     8821 acgctgaaag aaaaagggct gatttttgtt ggtctggata tcatcggcga ccgtctgact
     8881 gaaattaacg tcaccagccc aacctgtatt cgtgagattg aagcagagtt tccggtgtcg
     8941 atcaccggaa tgttaatgga tgccatcgaa gcacgtttac agcagcagta acccacctta
     9001 gcgagaagga tctcgttgag actctgagtg acagcgccct tctttccacg catactgggc
     9061 gctgttgctt ttttgaacca ggaaacagaa cctctgacaa tgaatttaca gcatcacttt
     9121 cttattgcca tgcctgctct ccaggatccg attttccgtc gttccgtggt ctacatttgc
     9181 gaacataata ccaatggtgc aatggggatc atcgtcaaca agccgctgga aaatctcaaa
     9241 attgaaggga ttctggaaaa gctgaagatc acgccggagc cgcgtgatga atcaatccgt
     9301 ctggataaac cggttatgct cggcggccca ctggctgaag atcgcgggtt tattttgcat
     9361 actccgccct ccaattttgc ttccagcatt cgcatttcag acaacacggt gatgaccact
     9421 tcccgcgatg tgctggaaac gctcggcacc gataaacaac cgtctgacgt attggtggct
     9481 ctgggctatg cctcctggga aaaaggtcaa ctggaacaag aaattctcga taacgcgtgg
     9541 ctaacggccc cggcggatct gaatattctg ttcaaaacgc cgattgccgg ccgctggcgc
     9601 gaggcggcaa aactgattgg tgtggatatt ctcaccatgc ctggtgtggc aggacacgcc
     9661 tgatgagtgg aaccttactc gccttcgact tcggcaccaa aagcattggc gtagcggtcg
     9721 gccaacgcat taccggcacc gctcgccctt tgcctgcaat taaagcgcag gacggtacgc
     9781 cggactggaa cctcatcgaa cgtttactga aagagtggca gcctgacgaa atcatcgtcg
     9841 gtttgccgct gaatatggac ggcaccgaac agccgttaac cgccagagcg cgcaaatttg
     9901 ccaaccgtat tcatggtcgt ttcggtgttg aagtaaagct ccatgacgag cgtcttagca
     9961 ctgtggaagc ccgttccggt ctttttgaac agggcggcta tcgggcgctc aacaaaggca
    10021 aagttgactc tgcctctgcg gttattattc tcgaaagcta tttcgagcag ggatattaag
    10081 gcgatctaaa agcctggcgg atagtcgtta ccgccatctt gccggatgcg gcgtgaacgc
    10141 cttatccagc acacatctgg cagcggctgt aggtctgata agacgcgtta gcgtcgcatc
    10201 agacatttat tgcctttata ggccctataa acttgcgcat cgggcatggc aacgtcacaa
    10261 acgcccttcc cccacccgct gctgataact ctgctgaaac gttaacatcc ccacctgctg
    10321 cccggtttga ataacatgcg gtaactggtg ggttttccct tcgcgaatca aattccccac
    10381 cgcgggagtg ttaatcagta attcaaacag cgccacgcgt ccttcctgtt tatccacttc
    10441 cagcttttgt gacagcactg cccgtaaact acccgcaagt tgattacgta cgggatcttt
    10501 ttcctgcgcc ggaaatgaat ccaccagtcg ctcaactgcc tgcgcggcac cacgtgtatg
    10561 taaggttgcc agcaccagat gcccggtttc tgccgccgtc agcgccagac ggattgtctc
    10621 actgtcacgt agctctccga gcaaaatcac atcaggatct tcccgcaatg cagcccgcaa
    10681 ccccgatgcg aacgtcatac agtgcaaacc aatttcccgc tgctggatca aacatcgctg
    10741 gctggcatag agatattcca caggatcttc cagcgtcaga atatgcgcat cggcatgttg
    10801 attaagatag ccaaccatcg ccgccagcgt ggtagatttg ccactccccg tagcccccgt
    10861 caccagaatc aggccattct cgctcttgag taattccggc aataccggtg gtgcaccaag
    10921 ctgttcgagc tgtggacagt gcgaaggtaa caaccgtaat gccagagaaa taccttgccg
    10981 ttgcgcgaac gcactgccac gcaaccgctg gttttccgcc agcgacacgg caaaatccag
    11041 ctggccattt tccagcaata ttgcccgctg atcgtcatcc agccactccc gcagtagctc
    11101 ttcgacgtcc ggcgcatcaa acggcgcagc ttccattctg ccacgaatgc gccatcgtgc
    11161 gggccaggcg ctgcacaggt gtagatccga gacgttatgc tttacactaa gggccacaat
    11221 ttcttccata ttcatactaa gatcctcgga aaatgaacga tattgcgcat aacctggcac
    11281 aggtccggga caaaatctca gcggctgcaa cgcgttgcgg ccgttctcca gaagaaatta
    11341 cgctgcttgc agtcagtaaa acaaaacctg cgagcgccat cgcagaagcc attgatgccg
    11401 ggcagcgtca atttggtgaa aactacgttc aggaaggggt agataaaatt cgccactttc
    11461 aggaactggg cgtaacagga ttagaatggc attttattgg cccgttgcag tctaataaaa
    11521 gccgcctagt ggcagagcat ttcgactggt gtcataccat cgaccgtttg cgcattgcta
    11581 cccgcctcaa cgagcagcgc ccggcagaac ttccccctct taacgtactg attcaaatta
    11641 atattagtga tgaaaacagt aagtccggga ttcaactggc agaactggac gagctggcag
    11701 cggcggtcgc tgaactaccg cgtttacgtc tgcgcgggct gatggcaatc cctgcgcctg
    11761 agtcagaata tgtaaggcag tttgaagttg cacgccaaat ggctgtagca tttgccggac
    11821 tgaaaacgcg ctacccgcat atcgacacgc tctctctggg aatgtcggac gatatggaag
    11881 ccgccattgc ggcaggtagc acgatggttc gtatcggcac tgcaattttt ggtgcgcgtg
    11941 attactctaa aaaataagga attaaaggaa cgccatgaat acgttgactt tcctgctttc
    12001 aacggtcatt gagctgtata caatggtgct gttattacgc atctggatgc agtgggctca
    12061 ttgtgatttt tacaacccct tctcacagtt tgtagtgaag gtagcgcagc caattatcgg
    12121 gccactgcgc cgcgttattc cggcaatggg tcccattgac agcgcctcgc tgctggttgc
    12181 ctatattctc agttttatca aagccattgt gctgtttgaa gtggtgacct tcctgccaat
    12241 catctggatt gccggtttac tgattctgct gaaaaccatc ggcttgctga ttttctgggt
    12301 cctgctggtg atggcgatta tgagctgggt aagccagggt cgtagcccga ttgaatacgt
    12361 gctgattcag ctggccgatc cgctgctgcg cccgattcgc cgcttgctac cggcaatggg
    12421 tgggattgat ttctcgccga tgatcctcgt tctgctgctg tatgccatca atatgggtgt
    12481 cgcagaagta ttacaggcaa ccggaaacat gctgctgccg gggctgtgga tggcgttatg
    12541 agtgccgtaa cagttaatga tgacggtctg gttttacagc tctatattca gccgaaagcc
    12601 agccgtgatt ctattgtcgg tttacatggc gacgaagtta aagtcgccat taccgcgccg
    12661 ccggttgacg gccaggccaa cagtcatctg gtgaagtttc tcggtaagca attccgggtt
    12721 gccaaaagcc aggtggtgat tgaaaaaggc gaacttggcc gccacaaaca aattaaaatc
    12781 attaatccgc aacaaatccc gccagaaatc gcggcgttaa ttaattaggt atcctatgca
    12841 aaaagttgtc ctcgcaaccg gcaatgccgg taaagtgcgt gagctggcgt cgctgcttag
    12901 cgactttggt cttgatatcg tggcccaaac agacctcggc gttgattccg ctgaagaaac
    12961 cggcctaacc tttatcgaaa acgcgattct gaaagcgcgc catgcggcaa aagtgaccgg
    13021 tttaccggca attgccgacg actctggtct ggcggtagat gcgctgggtg gcgcgccggg
    13081 tatttactcc gcgcgttatt ccggtgaaga cgcgaccgat caaaagaatc tgcaaaaact
    13141 gttggaaaca ctgaaagacg taccggacga ccaacgtcag gcgcgtttcc actgcgtgct
    13201 ggtatatctg cgtcacgcgg aagatccgac tccgctggta tgccacggca gctggccggg
    13261 cgtgattact cgcgaaccag cgggcactgg tggctttggt tatgatccaa tcttcttcgt
    13321 accttccgaa gggaaaaccg ccgccgaact gacccgcgaa gaaaagagcg ccatttccca
    13381 ccgtggtcag gcgttgaaac tgctgctgga cgctttacgt aatggttaaa ttaccgccgc
    13441 tgagtctcta cattcacatc ccgtggtgcg tgcagaaatg cccgtactgc gatttcaact
    13501 ctcacgcgtt gaaaggagaa gtgccgcacg acgattatgt tcagcatctg ctttgcgatc
    13561 tggacaatga tgtggcttac gctcagggcc gtgaagtaaa gacaatcttt attggcggtg
    13621 gtacgccgag cctgctttcc ggcccggcga tgcaaacgct gctagacggc gtgcgtgcgc
    13681 gtttgccgct ggcagcggat gcagaaatta ctatggaagc gaacccaggc acggtagaag
    13741 cggatcgctt tgtcgattat cagcgtgctg gtgtaaaccg catctctatt ggtgtgcaga
    13801 gttttagcga agaaaagctg aaacgacttg ggcgtattca tggcccgcaa gaagcgaaac
    13861 gagcggcaaa gctggcgagc ggtttagggt tacgtagctt taaccttgat ttgatgcatg
    13921 gactaccgga tcaatcactg gaagaggcgc ttggcgatct acgccaggcc attgaactga
    13981 atccgccgca tctttcctgg tatcaactga ctatcgaacc taatacgctg tttggctcgc
    14041 gaccgccggt gctgccggac gatgacgcgc tgtgggatat tttcgaacag gggcatcagt
    14101 tattaaccgc agcgggttat cagcaatatg aaacttccgc ttacgccaaa cccggttatc
    14161 agtgccagca caatctcaac tactggcgct ttggtgacta catcggtatt ggctgcggcg
    14221 cgcacggcaa agtgaccttc ccggatgggc gcattctgcg taccactaaa acgcgtcatc
    14281 ctcgtggttt tatgcaagga aggtatctgg aaagccagcg tgatgtcgaa gccgcagata
    14341 agccgtttga gttctttatg aatcgcttcc gtctgctgga ggccgcgccg cgcgtggagt
    14401 ttattgcgta taccgggctt tgcgaagatg tgattcgccc acagttagac gaggcgattg
    14461 cccagggtta tctcaccgaa tgtgcggatt actggcagat aacggaacat ggtaaattat
    14521 tcttaaactc acttcttgaa ttattcctcg ctgagtaatc attaatgcgc ctgcgggcgc
    14581 attaatcact tcttttatcc ccccgttaat taacctcggc agaaatatat ttcttactga
    14641 ataaatatcg acgacattta ttatatatat ttagtgaata tttactaatc attcattgat
    14701 tataaatatg tacaacctgg cgcacaaatt taatatttaa tttctgactt tatttcaagt
    14761 tgtgataaac atagacaagt tgtcagacag gttaaccaat ttgtcgcaca aatcactgat
    14821 aagcacaatt agatatcaac caatttccat ccttcaacag ggagtttttt tatgaaaatg
    14881 aggtcattta cccgctcatt agtctgcgca tccttgctgg ctctggttag cactggcgta
    14941 aacgccgcag aaaaagtggc gttaaagtta gcacacaacc tggaaagaag tcatgttgta
    15001 catcagagtt ttgaagagct ggcgaaagag gtaaaacagc tgtcaaaagg taatatggtg
    15061 atccgtattt atcccagcag ccagatgggt aatgcgcgag aaactatgga attgttgcaa
    15121 aatggcgcac tggatatgac caaaggttcc gccagtgatc tggagtcttt tgataatatt
    15181 tatgctatct ataatttgcc gttcttatta agttgttttt ggtgaagttg gtaaagaaat
    15241 tatggactca acaaaagaca aaggcttttt tgctttatca gcttatgtag cgggtactcg
    15301 cagtttctat gcgaaaaaac cgattacaaa gccagaagat cttaaaggac tgaaaattcg
    15361 tgttcagcca agtccgacca ccatcaaaat gattgaactg atgggtggtt cacccactcc
    15421 aatttcattt ggcgaagttt atactgcgat gcagcaaggt gtagtagatg gcgcagaaaa
    15481 taacgtgcct tcctggatgc agactcgaca tattgaaatt gccaaagttt tttctgaaga
    15541 tgaacacgcc tcaattcctg actttttggt tatctccacc aaaacatgga ataaattaac
    15601 cccagaacag caacaaatcc tcgaaacagc ggcgaagaaa tccgaagctt atcaacaaaa
    15661 attgtgggaa aaaattgacg ctgacacacg tgctcaggcg aaagctatgg ggggtgaaat
    15721 tgtaaaagtg gataaagcgc cattccgtaa agccgtgcaa cctctgtttg acgacttcaa
    15781 aaaagatcca aaacaagctg ctttgttaga gaaattcgac aacgctgctc aataatattc
    15841 cgggccagcc tctggccctg attttccagg atgcttacaa tgatatttaa ccgcctgaaa
    15901 cttgcggtag accgcgtcat tgccgcgttt tctgtcgccg taatgcttgc tctggttgta
    15961 tgcgtagtct ggcaagtatt tagccgctac gtacttaacc agccaagtac attaaccgat
    16021 gagctggcac gctttttgat gatctgggta ggattattag gtgctgcata taccgtcggc
    16081 gcgcagcgac atctctctat tgatttattt gctctcgccc tgaataagcg caaacaatta
    16141 cttcttagta tagtcattaa cgttctcatt ttaggttttg ccggttcggt cattgtgact
    16201 ggtggcctga aattaattga taaaacactg gcaacatcac aggtatctgc cgctatgcaa
    16261 ataccgatgg gatacgtata tatcattctg ccattaagtg gagtggtaat gatgttttat
    16321 gccttgtgtt ttattaatca aagcattcaa caactgaaac aacctgcgca ggaggccagt
    16381 taatgg
//
LOCUS       DAEEUE010000085        16309 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.085, whole genome shotgun sequence.
ACCESSION   DAEEUE010000085 DAEEUE010000000
VERSION     DAEEUE010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16309
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.085"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..246)
                     /gene="yefM"
                     /locus_tag="KLK84_003005"
     CDS             complement(<1..246)
                     /gene="yefM"
                     /locus_tag="KLK84_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HBE2010251.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            529..570
                     /gene="hisL"
                     /locus_tag="KLK84_003006"
     CDS             529..570
                     /gene="hisL"
                     /locus_tag="KLK84_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HBE2010252.1"
                     /translation="MTRVQFKHHHHPD"
     misc_feature    546..669
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            716..1615
                     /gene="hisG"
                     /locus_tag="KLK84_003007"
     CDS             716..1615
                     /gene="hisG"
                     /locus_tag="KLK84_003007"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBE2010253.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1621..2925
                     /gene="hisD"
                     /locus_tag="KLK84_003008"
     CDS             1621..2925
                     /gene="hisD"
                     /locus_tag="KLK84_003008"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBE2010254.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2922..3992
                     /gene="hisC"
                     /locus_tag="KLK84_003009"
     CDS             2922..3992
                     /gene="hisC"
                     /locus_tag="KLK84_003009"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBE2010255.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3992..5059
                     /gene="hisB"
                     /locus_tag="KLK84_003010"
     CDS             3992..5059
                     /gene="hisB"
                     /locus_tag="KLK84_003010"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HBE2010256.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5059..5649
                     /gene="hisH"
                     /locus_tag="KLK84_003011"
     CDS             5059..5649
                     /gene="hisH"
                     /locus_tag="KLK84_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBE2010257.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5649..6386
                     /gene="hisA"
                     /locus_tag="KLK84_003012"
     CDS             5649..6386
                     /gene="hisA"
                     /locus_tag="KLK84_003012"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBE2010258.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6368..7144
                     /gene="hisF"
                     /locus_tag="KLK84_003013"
     CDS             6368..7144
                     /gene="hisF"
                     /locus_tag="KLK84_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBE2010259.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7138..7749
                     /locus_tag="KLK84_003014"
     CDS             7138..7749
                     /locus_tag="KLK84_003014"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBE2010260.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7935..8912)
                     /gene="wzzB"
                     /locus_tag="KLK84_003015"
     CDS             complement(7935..8912)
                     /gene="wzzB"
                     /locus_tag="KLK84_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HBE2010261.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9058..10178)
                     /gene="ugd"
                     /locus_tag="KLK84_003016"
                     /pseudo
     CDS             complement(9058..10178)
                     /gene="ugd"
                     /locus_tag="KLK84_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10428..11834)
                     /gene="gndA"
                     /locus_tag="KLK84_003017"
     CDS             complement(10428..11834)
                     /gene="gndA"
                     /locus_tag="KLK84_003017"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBE2010262.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11943..12302)
                     /locus_tag="KLK84_003018"
     CDS             complement(11943..12302)
                     /locus_tag="KLK84_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010263.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12299..12805)
                     /locus_tag="KLK84_003019"
     CDS             complement(12299..12805)
                     /locus_tag="KLK84_003019"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010264.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILN
                     SNIFILNT"
     gene            complement(12951..13514)
                     /locus_tag="KLK84_003020"
     CDS             complement(12951..13514)
                     /locus_tag="KLK84_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010265.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13721..14659)
                     /locus_tag="KLK84_003021"
                     /pseudo
     CDS             complement(13721..14659)
                     /locus_tag="KLK84_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14722..15096)
                     /locus_tag="KLK84_003022"
     CDS             complement(14722..15096)
                     /locus_tag="KLK84_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HBE2010266.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15100..16248)
                     /gene="wzy"
                     /locus_tag="KLK84_003023"
     CDS             complement(15100..16248)
                     /gene="wzy"
                     /locus_tag="KLK84_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HBE2010267.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
BASE COUNT         4700 a         3835 c         3624 g         4150 t
ORIGIN      
        1 aataatgtcc ttttccgttc ctttgcctga tttcaggcta tcgattgagt ccatcaatct
       61 ccgggcgtta gcgggggaac gcagtagata agccgtctct tccagcgaat tgtattcttc
      121 gagtgacatc agaacacaag cctctccatt ctgacgagta atgaggatcg gggcatgatc
      181 ttcaacggct ttcatcattg ttgccgacaa attctgacgc gcttcgctgt agctaattgt
      241 acgcatgtaa atctcctatt ttgtacagtt cattgtacaa tgataggtgt taattaacta
      301 tttattaatt agtttgtaga tcaagacatt gccagcgaga cgaaaaatca gactttgctc
      361 tttttggtgc tgtcaagtta gaggacagtc ctcttagccc cctcctttcc ccgctcattc
      421 attaaacaaa tccatcgcca taaaatatat aaaaaagccc ttgctttcta acgtgaaagt
      481 ggtttaggtt aaaagacatc agttgaataa acattcacag agatttttat gacacgcgtt
      541 caatttaaac accaccatca tcctgactag tctttcaggc gatgtgtgct ggaagacatt
      601 cagatcttcc agtggtgcat gaacgcatga gaaagccccc ggaagatcac cttccggggg
      661 cttttttatt gcgcggttgt taacggttca gacaggttta aagaggaata agaaaatgac
      721 agacaacact cgtttacgca tagctatgca gaaatccggc cgtttaagtg atgactcacg
      781 cgaattactg gcgcgctgtg gcattaaaat caatcttcac acccagcgcc tgatcgcgat
      841 ggcagaaaac atgccgattg atattctgcg cgtgcgtgac gatgacattc cctgtctggt
      901 aatggacggc gtggtagacc ttgggattat cggcgaaaac gtgctggaag aagagctgct
      961 taaccgccgc gcccagggtg aagatccgcg ctactttacc ctgcgtcgtc tggatttcgg
     1021 cggctgccgc ctttcgctgg caacgccggt tgatgaagcc tgggacggtc cgctctcctt
     1081 aaacggtaaa cgtatcgcca cctcttatcc tcacctgctc aagcgttatc tcgaccagaa
     1141 aggtatctct tttaaatcct gcttactgaa cggttctgtc gaagttgcgc cgcgtgccgg
     1201 gctggcggat gcgatttgcg atctggtttc caccggtgcc acgctggaag ctaacggcct
     1261 gcgcgaagtc gaagttatct accgctcgaa agcctgcctg atccagcgcg atggcgaaat
     1321 ggaagaatct aaacagcaac tgatcgacaa gctactgacc cgtattcagg gtgtgatcca
     1381 ggcgcgcgaa tcaaaataca tcatgatgca cgcaccgacc gaacgcctgg atgaagtcat
     1441 cgccctgctg ccaggtgccg aacgcccaac cattctgccg ctggcgggtg atcaacagcg
     1501 cgtagcgatg cacatggtga gtagcgaaac cctgttctgg gaaacgatgg aaaaactgaa
     1561 agcactgggt gccagttcaa tcctggtcct gccgattgag aagatgatgg agtgatcgcc
     1621 atgagcttta acacaatcat tgactggaat agctgtactg cggagcaaca acgccagctg
     1681 ttaatgcgcc cggcgatctc cgcttctgaa agcattaccc gcaccgttaa cgatattctc
     1741 gataacgtga aaacgcgtgg cgatgaggcc ctgcgtgaat acagcgcgaa gtttgataaa
     1801 accacggtta ccgcgctgaa ggtgtctgct gatgagatcg ccgccgccag cgaacgcctg
     1861 agcgacgagc taaaacaggc gatggcggtg gcagtaaaga gtattgaaac cttccacact
     1921 gcgcaaaaac tgccgccggt agatgtagaa acgcagctag gcgtgcgttg ccagcaggtc
     1981 acgcgcccgg tggcttcagt gggtttgtat attccgggcg gctccgcccc gctcttctca
     2041 acggtattaa tgctggcaac tccggcgcgt attgcgggct gtaaaaaagt ggtgctgtgc
     2101 tcaccgccgc cgattgccga tgaaattctt tatgcggcgc agctgtgcga tgtgcaggac
     2161 gtgtttaacg tcggcggcgc acaggccatt gccgcgctgg cgtttggtac ggaatctgtg
     2221 ccgaaagtgg acaaaatctt cgggccgggt aacgcctttg tcaccgaagc aaaacgccag
     2281 gtaagccagc gtctggacgg tgcggcgatc gatatgcccg caggcccgtc ggaagtgctg
     2341 gtgattgctg acagcggcgc tacgccggat tttgtggctt ctgatttgct ttctcaggct
     2401 gaacacggcc ctgactcaca ggtaatttta ctgacccccg atgctgatat ggcgcgtcgc
     2461 gttgccgagg ccgtcgaacg ccaactggca gaactgccgc gtgccgaaac cacccgccag
     2521 gcactgaacg ccagccgcct gatcgtgact aaagatttag cgcagtgcgt ggagatctcc
     2581 aaccagtacg gcccggagca cctgatcatt cagacccgca acgcccgcga actggtcgat
     2641 ggcatcacca gcgccggttc ggtatttctt ggtgactggt caccggaatc ggcaggcgac
     2701 tacgcctccg gcaccaacca cgtgctgccg acttacggtt acaccgccac ctgttccagc
     2761 ctcgggctgg cagatttcca gaaacgcatg accgtacagg aactgtcgaa agaaggtttc
     2821 tccgcgctgg cttcgaccat tgaaacactg gccgccgccg agcgcctgac cgcccacaaa
     2881 aatgccgtta ctttgcgtgt taacgtcctt aaggagcaag catgagcacc gtgactatta
     2941 ctgatttagc gcgtgaaaac gtccgcaacc tgacaccgta tcagtcggcg cgtcgtctgg
     3001 gcggtaacgg cgacgtctgg ctgaacgcca acgaatatcc cacagccgtg gagtttaagc
     3061 ttactcagca aacgctcaac cgctacccgg aatgccagcc aaaagcggtg attgaaaatt
     3121 acgcgcaata tgcaggcgta aaaccggaac aggtgctggt cagccgtggc gcggacgaag
     3181 gtattgaact gctgatccgc gctttttgcg aaccgggtaa agacgccatc ctctactgcc
     3241 cgccaacgta cggcatgtac agcgtcagcg ctgaaaccat tggtgtcgaa tgccgcacag
     3301 tgccgacgct ggaaaactgg caactggact tacagggcat ttccgacaag ctggacggcg
     3361 taaaagtggt ctatgtttgc agccccaaca accccaccgg gcaactgatc aatccgcagg
     3421 attttcgcac cctgctggag ctaacccgcg gtaaggcgat tgtggttgcc gatgaagctt
     3481 atatcgagtt ttgcccgcag gcatcgttag ctggctggct ggcggaatat ccgcacctgg
     3541 cgattttgcg taccctttcg aaagccttcg cgttggcggg gcttcgttgc ggatttacgc
     3601 tggcaaacga agaagtcatc aacctgctga tgaaagtgat ccccccctac ccgctctcga
     3661 cgccggttgc cgacattgcc gcacaggcgt taagcccgca gggaatcgtc gccatgagcg
     3721 aacgggtggc gcaaattatt acagaacgcg aatacctgat tgccgcactg aaagagatcc
     3781 cctgcgtgga gcaggttttc gactctgaaa ccaactacat tctggcgcgc tttaaagcct
     3841 ccagtgcagt gtttaaatct ttgtgggatc agggcattat cttacgtgat cagaataaac
     3901 aaccctcttt aagcggctgc ctgcgaatta ccgtcggaac ccgtgaagaa agccagcgcg
     3961 tcattgacgc cttacgtgcg gagcaagttt gatgagtcag aagtatcttt ttatcgatcg
     4021 cgatggaacc ctgattagcg aaccgccgag tgattttcag gtggaccgtt ttgacaaact
     4081 cgcctttgaa ccgggcgtga tcccggaatt gctgaagctg caaaaagcgg gctacaagct
     4141 ggtgatgatc actaatcagg atggtctggg aacacaaagt ttcccgcaag cggattttga
     4201 tggcccgcac aacctgatga tgcagatctt cacctcgcaa ggcgtgcagt ttgatgaagt
     4261 gctgatttgt ccgcacctgc ccgccgatga gtgcgactgc cgtaagccga aagtaaaact
     4321 ggtggagggt tatctggctg agcaagcgat ggatcgtgcc aacagttatg taattggcga
     4381 tcgcgcgacc gacattcaac tggcggaaaa catgggcatt aatggtttac gctacgaccg
     4441 cgaaaccctg aactggccaa tgattggcga gcaactcact aaacgagacc gttacgccca
     4501 tgtagtgcgc aacaccaaag agacgcagat tgacgtccag gtgtggctgg atcgcgaagg
     4561 tggcagcaag attaacaccg gcgttggctt ctttgatcac atgctggatc agatcgccac
     4621 ccacggcggt ttccgcatgg aaatcaatgt caaaggcgac ctctatatcg acgatcacca
     4681 caccgtcgaa gataccggcc tggcgctggg cgaagcgtta aaaattgccc tcggcgacaa
     4741 acgcggtatt tgccgctttg gttttgtgct gccgatggac gaatgccttg cccgctgcgc
     4801 actggatatc tctggtcgcc cgcacctgga atataaagcc gagtttacct accagcgtgt
     4861 gggcgatctc agcactgaaa tgatcgaaca cttcttccgt tcgctctctt acaccatggg
     4921 cgtgacacta cacctgaaaa ccaaaggtaa aaacgatcat caccgtgtag aaagcctgtt
     4981 caaagccttt ggtcgtaccc tgcgccaggc catccgcgtc gaaggcgaca ccctgccctc
     5041 gtcgaaagga gtgctgtaat gaacgtggtg atccttgata ctggctgcgc caacctgaac
     5101 tctgtgaagt ctgccattgc gcgccacggt tatgaaccca aagtcagccg tgacccggac
     5161 attgtgttgc tggccgataa actgttttta cccggcgtcg gcaccgcaca agcagcgatg
     5221 gatcaggtgc gtgagcgcga gctgtttgat ctcatcaaag cctgtaccca accggtgctg
     5281 ggcatctgct tagggatgca actcctggga cgacgcagcg aagagagcaa tggcgtcgac
     5341 ttgctgggca tcatcgacga agacgtgccg aaaatgaccg actttggtct gccactgcca
     5401 catatgggct ggaaccgcgt ttatccgcag gcaggcaacc gcctgtttca ggggattgaa
     5461 gacggcgcgt acttttactt tgttcacagc tacgcaatgc cagtcaatcc gtggaccatc
     5521 gcccagtgta attacggcga accgttcacc gcggcggtac aaaaagataa cttctacggc
     5581 gtgcagttcc acccggagcg ttctggtgcc gctggcgcta agttgctgaa aaacttcctg
     5641 gagatgtgat gattattccg gcattagatt taatcgacgg caccgtggtg cgtctccatc
     5701 agggcgatta cggcaaacaa cgcgattacg gtaacgaccc gctgccgcgc ttacaggatt
     5761 acgctgcgca aggtgccgaa gtgttgcacc tggtggatct gaccggggca aaagatccgg
     5821 ctaagcgtca aatcccgctg attaaaaccc tggtcgcggg cgttaacgtc ccggtgcagg
     5881 ttggcggcgg tgttcgtagt gaagaagacg tggcggcgtt actggaagct ggcgttgcgc
     5941 gcgtggtggt cggttccacc gcagtgaaat caccggacgt agtgaaaggc tggtttgaac
     6001 gtttcggtgc cgatgcgctg gtgctggcac tggatgtccg tattgacgag caaggcaaca
     6061 agcaagtggc agtcagcggc tggcaagaga actcgggcgt ttcactggaa caactggtgg
     6121 aaacctatct gcccgtcggc ctgaaacatg tgttgtgtac cgatatctcg cgcgacggca
     6181 cgctggcggg atctaacgtc tctttatatg aagaagtgtg tgccagatat ccgcaagtgg
     6241 cgtttcagtc ctccggcggc attggcgaca ttaacgatgt tgctgcccta cgtggcactg
     6301 gcgtgcgcgg cgtaatagtt ggtcgtgcat tactggaagg taaattcacc gtgaaggagg
     6361 ccatcgcatg ctggcaaaac gcataatccc gtgtctcgac gttcgtgatg gtcaggtggt
     6421 gaaaggcgta cagtttcgca accatgaaat cattggcgat atcgtgccac tggcaaaacg
     6481 ctacgccgaa gaaggtgcag atgaactggt gttctacgat atcaccgctt ccagcgatgg
     6541 ccgtgtggta gataaaagct gggtatctcg cgtggcggaa gtgatcgaca ttccgttttg
     6601 tgtggcgggt gggattaagt ctctggaaga tgccgcgaaa attctttcct ttggcgcgga
     6661 taaaatttcc atcaactccc ctgcgctggc agacccaaca ttaattactc gcctagccga
     6721 tcgctttggc gtgcagtgta ttgtggttgg tattgatacc tggtacgacg ccgaaaccgg
     6781 taaatatcat gtaaatcaat ataccggcga tgaaagccgc acccgcgtca ctcaatggga
     6841 aacgctcgac tgggtagagg aggtgcaaaa acgcggcgcc ggagaaatcg tcctcaacat
     6901 gatgaatcag gacggcgtgt gtaacggtta cgacctcaag cagctgaaaa aagtgcgtga
     6961 agtttgccac gtcccgctga ttgcctccgg tggcgcgggc accatggaac acttcctgga
     7021 agccttccgc gatgccgacg ttgacggtgc gctggcagct tccgtattcc acaaacaaat
     7081 aatcaatatt ggcgaattaa aagcgtacct ggcaacacag ggcgtggaga tcaggatatg
     7141 ttaacagaac aacaacgtcg cgaactggac tgggaaaaaa ccgacggtct gatgccggtg
     7201 attgtgcaac acgcggtatc tggcgaagtg ttaatgctgg gctacatgaa cccggaagcc
     7261 ttagacaaaa ccatcgaaag cggtaaagtc accttcttct cgcgcactaa acagcgactg
     7321 tggaccaaag gcgaaacctc gggcaatttt ctcaatgtgg taagtattgc cccggactgc
     7381 gacaacgaca cattactggt gctggcgaat cccatcgggc caacctgcca caaaggcacc
     7441 agtagctgct tcggcaacac cgctcaccag tggctgttcc tgtatcaact ggaacaactg
     7501 ctcgccgaac gtaaatatgc cgacccggaa acctcctaca ccgccaaact gtatgccagc
     7561 ggcactaaac gcattgcgca gaaagtgggc gaagaaggcg tggaaaccgc actggcagca
     7621 acggtacatg atcgctttga gctgaccaac gaggcatctg acttgatgta tcacctgctg
     7681 gtgctgttgc aggatcagga tctggattta acgacagtaa ttgagaacct gcataaacgg
     7741 catcagtgag ttacgggctg agcaggtgtg atgttgttgt cacacccgga gccattgccc
     7801 gatgcgcaaa cttatcgtgc ctacacatta tctcaacaca cgtaggccgg ataagatgcg
     7861 ccagcatcgc atccggcaaa aaaaacgggc atggtgtcac caccctgccc gtttctctta
     7921 aatgcacaat aatattactt cgcgttgtaa ttacgcagag cgttacgccc caacacaatc
     7981 cccgcgccaa ccatgccgcc cagaagcact gccagaatca aggtgattgc ctttttcgga
     8041 ctatcgcgac gaataggtaa cgtcggtttc atcacatagc ggtaagcatg aatatcaaga
     8101 tcatcaaact ttaatttttc aatatccaac aggttttgac gtgtctgata gtagtttggt
     8161 gagaacacca acggacgagt cgcttcatgc ttaatcatcg actccagcgc ttcgctccct
     8221 agaaggaaca acgtatcttg cgtcacatct tcagtctgct gaacctgtgg ctttgtcacc
     8281 tgcgcctgat tcgcatactg caacgcttcc tgaatctgac ggatacgcag atctttctgc
     8341 tcctgcgcga ccacttcctg ggttcttaaa gagtcctgca agtttttccg tcccagagca
     8401 atgttgtcct tgagatcctt ttctagctct tgattcactt tatcatcaac ttgctgaatg
     8461 tattgggcca acttcatttg tgcgccctct gcagtttgcc caacataaga aacagtcaat
     8521 ggtaattgct ggttcttaac agaaggttcg atggtaagtt tttctggctc ttcctgatta
     8581 tccagcgttt ctgctaatgc agagaaggca gaactgaagc gaccaattaa cgtctcctgc
     8641 aaatccgata ctttcggtgc agcctgacca tagataacat tcatggcatt gttatagcca
     8701 gcaatttgcc ccacgtcggg ctgagtgata attgctgttg acgtccattt ctccttcgct
     8761 actgccaaat aaccaatagc cagggcaata gccacaatga cggaaatgat aattgtcatc
     8821 ttgccacgcc acaactgcac tagtaaatca atcaaatcaa tctgttccgg gtcatggttt
     8881 tgcccagaaa cattattatt ttctactctc atcattaccc taactgacgg aaatttccaa
     8941 gtccgcatag tttagcgata atccccgtga atgctatagg ataagtgata aaaataatga
     9001 attattgcgg aggtaacaaa agacaatcag ggcgtaaata gccctgataa caagatgtta
     9061 gtcgctgcca aagagatcgc gggtgtagac cttatctgcc acatccttaa gctcttctgc
     9121 catacggttg gagataatga cgtcggcttg ttgtttgaag gtggcgagat cacgttccag
     9181 gcgagagttg aagaatgagt cttctttcat caccggctcg tagatgatca cttcaacgcc
     9241 tttcgccttg atacgcttca taatcccctg aatagaagac gcacggaagt tatctgaccc
     9301 gctcttcata atcagacgat aaatacccac cacttgcggt ttgcatcgac aattgccgag
     9361 atcaggttat tcggcacaga ctggtagttc gccagtaact gcttggtatc tttcggcaga
     9421 caataaccac cataaccaaa cgacggattg ttgtaatggt tgccaatacg cggatcgaga
     9481 caaacgcctt cgataatctg gcgagtattc agacctaaac tttctgcata actatccagt
     9541 tcattaaagt atgctacgcg catcgccaga taggtattag cgaaaagttt aatcgcttct
     9601 gcttcagtgg agtcggtaaa cagggtcggg atattttgct taatcgcgcc ttcctgtaac
     9661 aacgcagcaa aacgttctgc gcgttctgaa cgctcaccga tgacaatacg tgacggatgg
     9721 agattatcgt aaagggcttt accttcgcgg aggaattccg gagagaaaat aatattttca
     9781 gtacgatatt tcttatgcat cgctgcggta aaaccaacgg gaaccgttga tttgatgacc
     9841 ataaccgcat aaggatttat ctcaactacg tctttaatta ctgattctac actggatgta
     9901 ttgaaataat tagttttagg atcatagtcg gttggagtgg cgatgatgac ataatcagca
     9961 tcccggtagg cttcattttt atctaatgtg gcattaaagt gtattttatc tgattgcaaa
    10021 aactgctgaa tttccttatc aacaatagga gatatccgat cattcagcat agcaacgcgt
    10081 gacggtaaaa tatctaatgc cacaacctca tgattttgtg cgattagaag cccgtttgac
    10141 aagcctacat agccagtacc ggaaatggtg attttcattt catgctctca gaattaactt
    10201 aactgtgaat catgatgttt ttagcatcgt gataagatgc taaaagtttt aacgctacgt
    10261 tgtcagatgg tcaacgcaac gaaaaacaat tcagattttc tttataagaa tagctgatgt
    10321 ttattaagtt aatattaagc agtgaaattt agtctataag atatttggca aaaaaaagcc
    10381 cggtgcaata tacgccgggc ctcaatttta ttgttggtta aatcagatta atccagccat
    10441 tcagtatgga acacaccttc tttatcaatg cgcttataag tatgcgcccc gaaatagtca
    10501 cgctgtgcct ggatcaggtt cgcaggcaga accgcggcac ggtagctatc gtaataggca
    10561 accgcagcgg cgaaggtcgg aaccgggata ccgttctgta ctgcataagc gacgacatcg
    10621 cgcagcgcct gctggtagtc atcggcaatt tgcttgaagt acggggccag cagcaggtta
    10681 gcgatctgcg gattttcggc ataagcatcg gtgattttct gcaggaactg cgcacggatg
    10741 atgcagccag cacggaaaat cttcgcgatt tcgccgtagt tcagatccca attgtattct
    10801 tcagacgcag cacgcagctg agagaagccc tgagcgtaag aaacgatttt gcccagatac
    10861 agcgcactgc gaactttttc gatgaactca gccttgtcgc ctgctgactg agcttgcgga
    10921 ccagagagaa ctttagatgc ggcaacgcgc tgatctttca gagaagagat ataacgtgca
    10981 aacacagact cggtaatcag cgacagcggt tcgccgagat ccagcgcgct ctggctggtc
    11041 catttaccgg tgcctttgtt tgctgcttca tccagaatca catcaaccag gtagttaccg
    11101 tcttcatctt ttttggtgaa gatatcttta gtgatgtcga tcaggtagct gctaagttca
    11161 ccgttattcc actcggtgaa agtctgggcc agttcttcat tagatagatt caggccacct
    11221 ttgagcagag aatacgcttc agcaatgagc tgcatatctc cgtattcaat accattgtga
    11281 accatcttca cataatggcc tgcgccgtcg gcaccaatat aagtaacgca tggctcgccg
    11341 tcttctgcca cggcagcgat tttagtcagg ataggcgcaa ccagctcata agcctctttc
    11401 tgcccgccag gcataataga aggacctttc agcgcgccct cttcaccgcc ggaaacaccg
    11461 gtaccgatga agttaaagcc ctctgctgaa agctcacgat tacgacgaat ggtgtcctgg
    11521 aagaaggtat taccaccatc aatgatgatg tcacctttat cgaggtatgg tttcagggaa
    11581 tcaatcgcag catccgtgcc tgcacctgct ttcaccatta acaggatgcg acgaggcgtt
    11641 tccagagatt caacaaactc tttcaccgta tagtaaggag ccagtttctt gcctggattt
    11701 tcagcaatca cttcttccgt cttttcgcgg gagcggttga aaatagagac ggtataacca
    11761 cgactttcga tgttgagcgc aaggttgcgc cccatcactg ccataccgac tacgccgatc
    11821 tgttgctttg acattgttta ctcctgtctt ctatattatt aaccgtctat taaccgttat
    11881 attattaacc gtcgtgattt tcatcttcta atcaaggaat acatatggtt acagcatgct
    11941 tcttacttta tagcgataag tatgccattg ggtgtatcca taagaacttt gaattttgga
    12001 tacttttctg gattaaaatt agcatagctt cgtttagata taacaataaa cgaactatta
    12061 aaagatttca tcttctctat taaatcatcc tgtgggatta cctcacactc gggattacta
    12121 atagtccaat cactatatct tgctaatcca aagacgtcac caattgccat ccctttatag
    12181 aagtaaatac catcctccaa gcctaattgg ataagattat ctccatataa ctcactatat
    12241 tcacttgctt tcttcatcag ttcatagcta tagcgacttt ctaagcgtga attaaaattc
    12301 aagtattcag aataaaaata tttgaattca aaatacaacg atcgccccga aaaaaaataa
    12361 tacacacaag gataaaatat cgtcccaact tgtaatttgt aatgttgata tcttttatta
    12421 aacttgcaat tgagatcgca acctgcaata ctgacaatgg gtaaatgggt gataaatacc
    12481 tgtcaccttg cgtaacagaa aaccaaaata agacatatgt taaaacaatc gagaaaaata
    12541 cccaaagtat tttattattc ctaagtgtaa agacagacag tatagcataa aaaacaacat
    12601 cactgccaac tttattaatt gcttgttgca aatctaatgt ggcaggactt ataccatgtc
    12661 gagcttgttc tgctgtttga acttgtagat caatttcatt ccataggtag tgaccaaata
    12721 tatttcctcc agcaggacta aaaggatcac cagagatcca aaaactacgg acataccaat
    12781 aaatgccacc aagaattgtt gccaagctaa aaataagaaa tgtctgtatg tttctctttt
    12841 tcgctatcaa tataacaaat atgatggctc catatatggc accaaatagt tttacgccac
    12901 aagcaattcc aataaaaaaa ccacataaaa aaattaattc attttgtgtt ttattgtttt
    12961 ctgagcgaat atatataaga atactggctg cagcaaaaca aagatagcgt taaatcaata
    13021 tatgcataac caagatgatc tttaagtgga ttgatagtaa atagtaggat tgtagcaata
    13081 atgcctggat aataagtttt cgtaatttcc catgaacagc caacaagtcc acaggctatt
    13141 acaaaaaaac agatatttgc taaaaactga gctaacctta catctcctaa ctgtaaacct
    13201 aaagccatta aaagatctgc attttgcggg aacagaggaa aacgcaaata ttgttcaaca
    13261 acaattgcat ggtgctcaac taatgaacga gccaaaggta agtgatacat agtatcatcc
    13321 caatcaccgg gagctcgcag ggacgataac aatgtaacca caaaaatcaa aacaagaaat
    13381 aaaataacaa acagcgtact gaactcaata ttatttattg ccagccatct aaattgctga
    13441 cgtttaataa tgcttaaaac ccaacacacc gtaaaaatta aaaagatagc aatcttatta
    13501 aattgcccga ccatgcccag aaagtaaatt atcaaaatat ttaaagacat ccctatgcat
    13561 aggcaataaa aaataaagaa aatttgttaa gtcctgccac cccctcctta aaaaaagatg
    13621 acaatacaat attaccaatg acaaaaaatg tcattccaaa agcaaaacaa tacacaacat
    13681 acaaatacaa cggcaattca atcatagtaa aatactctat ttacattatc agtttatagt
    13741 ttttttcaga aagataaaat aatgccttca tttcacgtct agttgttgta tttgaatgca
    13801 aaataatgcc acatacaaat gccattattg acgataacat gatggaggca gttaaaaagg
    13861 cagtcggaaa tcttctcacc aaacctgtat caatataatc aacaatgatg ggcaatccta
    13921 aaattataga caaagcagca agtaaaacgc cgataatact aaaaaagtat agtggccgtt
    13981 caagcgagta aagtttaatt attgttttca gaatcttgaa cccatcactc caagttgata
    14041 gtttgctcac actcccttca gatcgctcgc catattttgt attaacctca ccatacttca
    14101 tacgtaattc taaagcatga attgtaagct cagtttcgat ttcaaatccc cttgatagac
    14161 atggaaaaga tttaacatag cgtctggaaa atacacgata ccccgacaac atatcagtaa
    14221 aattcccttt aaagatagtc tgaacagtac cagtcaacag tttatttcct cagcgatgcc
    14281 cttttctgta tgtattttga tctccattct cttgacgaca tcctacaacc atatccaatt
    14341 gattttttat gagatggtta atcatttctg gagcagaact tgcatcataa gtgtcatctc
    14401 catcaaccat tagataaata tccgcatcta catctgagaa catacgtctt acgacattcc
    14461 ccttgccttt cagaggaaca gagtgaactg ttgctccagc atcctctgcc acttgtgctg
    14521 tactgtcagt cgaattatta tcaaatacat atatagaagc agttggaata ttcgttttga
    14581 aatcatttat gacttttgct attgccttct gctcattata acaaggaata attacagcga
    14641 tattaaaaga gtgatacata agcccctcat ataaactatt tagctaatgt gcaagtaatt
    14701 gtgtttttaa caaatcgttt actaacgaaa aatgaaatat tttgatgcgc aatatcctaa
    14761 aaccaaacta aaaagagaaa aggtgaacaa tgtaaatatt ggagataaag ctaagagatc
    14821 aaataaaacg ccgaataaat aactcatcgc ccccataaaa aatataaata taaaatatct
    14881 agccttggtt gcagatactt taaatgagca tcttgcattt gcaaaaaaag aaaaggttgc
    14941 tgcacatata aaaccaacaa tgttacttcg gccttgacca aaagctaaat tattataaca
    15001 cgcataaaat accccccaat gtaaagcagt attgacaatt ccaattaaaa aataactaaa
    15061 aaaagaactg gtcaataact tccctatttt taacatcctt tattttgctc cagaagtgag
    15121 gttattacta atttggatat tttctataga aaatacccat aaaactacaa cgcctataat
    15181 aaaaattccc atgggtgtaa aaagaaatgg attagttata gaaacggtaa tactgaaaaa
    15241 aagtattaac gacatcatta atgatgtttt tgtactattt aaaaaatagc ctttcaaaaa
    15301 gataagcaac ataggaacta atgatataaa tacaccaata acgcctgttt tctgaagtat
    15361 ctcaagaggt actatttcaa tccttaatcg atctaatata aatgaaccaa atcctcttcc
    15421 aaacaagaac gtcgctaaat caacattttt ataataaaaa tataaatcat taaatctaac
    15481 agagtcagaa tcttcactgc gtgatccgac catgtaaagc ataattataa atagaacgct
    15541 gcccaatatt attataatag tatttaaata tagcttatgt aatttaaaac tggctaataa
    15601 cagtgataat gttgtaaaaa gtaatacacc tcttgattct gtcaataata tcgcaagcac
    15661 tattatgaga cattttaaat attttttgtc gacaaaactg ataatcgcac cgacaccaaa
    15721 aaagtagaag gatttggaaa aaaaagcccc atcgggacga aaaaagaact cgctagtcaa
    15781 tgataagtgt tcataaatta aattaaaatt taacaacccg aaattcaaca aaattatgta
    15841 tgaaatatat aacagtgatc catataatgc aaatgcatta actattttaa taaaaatctc
    15901 acatgaaaat acttttaaag tatatcttag tgtaaataga tatataagtg acagcataaa
    15961 aaggtatggt tgcacatcaa acagtatatc atttatttta ttaccagcta ataagccgac
    16021 aaagaagcca taaaaaggaa ataaaagcaa ccatataaaa agactgacac atttttgggt
    16081 gattgaaaaa ttgaatgtaa ctaaattaaa aattaaaagt ataaaactaa atacaaataa
    16141 taaatatcgc aaagggaata ttcccaatgg ctccagtaat cttccaccac caccaataat
    16201 cagttcaata cataaaaatg ttataaaaat tttatttata ttattcatat tcgtaaggtg
    16261 atgtttttta aaatatgttt aaaatcaaca ttaataaaat ataaatttt
//
LOCUS       DAEEUE010000086        16011 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.086, whole genome shotgun sequence.
ACCESSION   DAEEUE010000086 DAEEUE010000000
VERSION     DAEEUE010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16011
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.086"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            44..375
                     /locus_tag="KLK84_003024"
                     /pseudo
     CDS             44..375
                     /locus_tag="KLK84_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
     gene            522..1883
                     /gene="yegQ"
                     /locus_tag="KLK84_003025"
     CDS             522..1883
                     /gene="yegQ"
                     /locus_tag="KLK84_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HBE2010268.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(2225..2542)
                     /locus_tag="KLK84_003026"
     CDS             complement(2225..2542)
                     /locus_tag="KLK84_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010269.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            2948..3847
                     /gene="yegS"
                     /locus_tag="KLK84_003027"
     CDS             2948..3847
                     /gene="yegS"
                     /locus_tag="KLK84_003027"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HBE2010270.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(3929..4702)
                     /locus_tag="KLK84_003028"
     CDS             complement(3929..4702)
                     /locus_tag="KLK84_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBE2010271.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(4808..5848)
                     /gene="gatD"
                     /locus_tag="KLK84_003029"
     CDS             complement(4808..5848)
                     /gene="gatD"
                     /locus_tag="KLK84_003029"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBE2010272.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(5896..7251)
                     /locus_tag="KLK84_003030"
     CDS             complement(5896..7251)
                     /locus_tag="KLK84_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBE2010273.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     CDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
     gene            complement(7255..7539)
                     /gene="gatB"
                     /locus_tag="KLK84_003031"
     CDS             complement(7255..7539)
                     /gene="gatB"
                     /locus_tag="KLK84_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HBE2010274.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(7570..8022)
                     /gene="gatA"
                     /locus_tag="KLK84_003032"
     CDS             complement(7570..8022)
                     /gene="gatA"
                     /locus_tag="KLK84_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HBE2010275.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(8032..9294)
                     /gene="gatZ"
                     /locus_tag="KLK84_003033"
     CDS             complement(8032..9294)
                     /gene="gatZ"
                     /locus_tag="KLK84_003033"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HBE2010276.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            complement(9323..10177)
                     /gene="gatY"
                     /locus_tag="KLK84_003034"
     CDS             complement(9323..10177)
                     /gene="gatY"
                     /locus_tag="KLK84_003034"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBE2010277.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(10485..11537)
                     /gene="fbaB"
                     /locus_tag="KLK84_003035"
     CDS             complement(10485..11537)
                     /gene="fbaB"
                     /locus_tag="KLK84_003035"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HBE2010278.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            11794..13071
                     /locus_tag="KLK84_003036"
     CDS             11794..13071
                     /locus_tag="KLK84_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE2010279.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAICYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            13068..14071
                     /locus_tag="KLK84_003037"
                     /pseudo
     CDS             13068..14071
                     /locus_tag="KLK84_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            14068..15033
                     /locus_tag="KLK84_003038"
     CDS             14068..15033
                     /locus_tag="KLK84_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HBE2010280.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(15007..15753)
                     /locus_tag="KLK84_003039"
     CDS             complement(15007..15753)
                     /locus_tag="KLK84_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBE2010281.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLCGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(15801..>16011)
                     /locus_tag="KLK84_003040"
     CDS             complement(15801..>16011)
                     /locus_tag="KLK84_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HBE2010282.1"
                     /translation="LAGYFNEYPWWVAHYYQRRPDNDGMAWRFWQHSDRGQVDGINGP
                     VDFNVFNGTGMSCRHSLMGLKKRLK"
BASE COUNT         4002 a         4132 c         3823 g         4054 t
ORIGIN      
        1 ttagatgctg ttttcgataa gcaatttcaa tcaaggagtc attatggctg gttggtttga
       61 actcagtaag agcagtgata atcagttccg gtttgtgcta aaagcgggca atggtgagac
      121 tatcctcacc agcgagctgt atacctcaaa agcctctgcg gaaaagggca tcgcgtcggt
      181 gcgtagcaac agcccgcaag aagagcgcta tgagaaaaaa cggcaagcaa cggcaaattc
      241 tatttcaatc tgaaagccgc taatcatcaa attatcggct ccagccagat gtacgccacc
      301 gcgcaatctc gtgaaaccgg aattgcctcc gttaaagcca atggcacaag ccagacggtg
      361 aaagacaata cgtaattacg atgccgggcg tgggttgttg gcgtccggcg ctttaccaga
      421 tcccccctac agcgctacaa tgcccgccct taaagtgggg gcctccccta accgcttcat
      481 caggtgaagc ggatctgacc tgtcatcaga acgagagaat tatgtttaaa ccggaactcc
      541 tttccccggc gggaacgctg aaaaatatgc gttacgcttt cgcttatggc gcagatgctg
      601 tttatgcggg ccagccgcgt tactccctac gtgtgcgcaa caacgaattc aaccacgaaa
      661 atcttcagct cggcatcaat gaagcccacg cgctggggaa aaagttttat gtcgtggtca
      721 acattgcacc gcacaacgcc aagctgaaaa cctttatccg tgacctgaaa ccggtggtgg
      781 aaatggggcc ggatgcgctg attatgtccg atccagggct gattatgctg gtgcgtgagc
      841 acttccctga aatgccgatc cacctctcgg tgcaggctaa cgccgtgaac tgggcgacgg
      901 tgaaattctg gcagcaaatg ggactgaccc gcgtgatcct ctctcgcgag ctgtcgctgg
      961 aagagattga agagatccat aatcaggtgc cggatatgga gatcgagatc ttcgttcacg
     1021 gcgcactgtg catggcctac tccggtcgct gcctgctctc tggctatatc aacaagcgcg
     1081 acccgaacca gggcacctgc accaacgcct gccgctggga gtacaacgtc caggaaggga
     1141 aagaagatga cgttggcaac atcgtacaca agtacgagcc gattccggtg caaaatgttg
     1201 agccgacgct gggtatcggc gcgccaaccg acaaagtgtt tatgatcgaa gaagcccagc
     1261 gtccgggcga gtatatgacc gcgtttgaag atgagcacgg cacttacatc atgaactcga
     1321 aagatctgcg cgccatcgcc catgtagaac gcctgaccaa aatgggcgtg cattcgctga
     1381 aaatcgaagg tcgtaccaaa tctttctact attgcgcacg caccgcacag gtttaccgca
     1441 aagctatcga tgacgccgct gcgggaaaac cgttcgatac cagcctgctg gaaactctgg
     1501 aaggtctggc gcatcgtggc tataccgaag gtttcctgcg tcgtcatact cacgacgatt
     1561 atcagaacta cgaatacggt tattcagttt ctgaccgcca gcagtttgtt ggtgagttta
     1621 ccggtgagcg caagggggac ctcgcagcgg tagcggtgaa aaataaattc tccgttggcg
     1681 acagtcttga gctgatgacg ccgcaaggca acattaactt tacccttgag cacatggaaa
     1741 acgccaaagg cgaagctatg ccggtcgcac caggcgatgg ttatactgtg tggctcccgg
     1801 tcccgcagga tcttgagcta aattacgcgc tgctaatgcg taatttctcc ggggaaacca
     1861 cgcgtaaccc ccacggtaag tgattaattt cgattatttt tcccggatgg aaaattctta
     1921 gaaaccgatc acatacagct gcatttatta aggttatcat ccgtttcgct gaaaaacata
     1981 atccataaaa tgctagctgt accaggaacc acctccttag cctgtgtaat ctcccttaca
     2041 cgggcttatt ttttgtacat aagctgtaaa gaaggattga tccctgataa ccgtcttcca
     2101 ttgaccacat cgatagaatc ccccttcata gcacaatgcc tttcacgtaa cggtatcgtg
     2161 ctcgcccagg ttccggctac gcacagccag aacgcgcatg tttgacgctt acaaaaaaat
     2221 attctcactc tccacatttg aatgtcagac gagcgacgcc atgtaatcct gcaccttctg
     2281 tcttcaggtc aactatctgc atttttttgc cctgagtaac acagaaatgg gctgcatcat
     2341 tttttactat attttctgca ccagatattc tgcccctggc taaagaagct tcggcttcgg
     2401 tgtagtattg gttatcgagt ttacgctgaa tattactttt atatgcaaga ccaaatttac
     2461 cgatacttgt ttcatcatta tgcacagcac aaccagatat aagaaaaata ctaattaatg
     2521 atatagcagc tatctttttc atctcacctt cccccattaa ataccaacga cactctctag
     2581 tgtttaaata taataatggc atgattatta taattgaata ggattataat aaatgctctg
     2641 tacaacattt cctacataag taggaattac ggacattgag gcccttcagg gtaactccat
     2701 gggggcttta atatattata ttgaagatgc cactgtttag ttgaatatta ggtatatgct
     2761 cttttttgaa atttatcggt ggcagccgtt agtattcgct gtccccattg caagctcctg
     2821 gtggtaacca ctgaatcctc catacttgaa ctgacttttt atcctccgac tttcatcctg
     2881 ttctgactcc accttttgtt ttctgctcta cactatctac agaccaatca taaaggcaca
     2941 tacgatcatg gcagaatttc ccgccagctt actgattctt aatggtaaaa gtactgacaa
     3001 tctacccttg cgcgaagcaa ttatgctgtt gcgtgaggaa ggaatgacga tccatgtgcg
     3061 ggtcacctgg gagaaaggtg atgccgcacg atttgtagag gaggcccgga agttgggcgt
     3121 cgcaacggtg attgccggtg gtggcgatgg caccattaat gaagtttcta cggcgttgat
     3181 tcagtgtgag ggggatgaca tacccgcgct gggaattttg ccattaggaa ccgccaatga
     3241 ttttgccacc agtgtaggga ttcctgaggc actggataag gcgctgaaac tggcaattgc
     3301 cggtaatgcc attgcgatag atatggcgca ggtcaacaaa caaacctgtt ttattaatat
     3361 ggcgacaggc ggatttggga cgcgtattac cacagaaacg ccggaaaaat taaaagccgc
     3421 gctgggtggc gtctcttaca tcattcatgg cttaatgcgc atggatactc tgcaaccgga
     3481 ccgttgtgaa atccgcggtg aaaactttca ctggcaaggt gacgccctgg tcattggtat
     3541 tggtaacggg cgtcaggccg gtggcggtca acaattgtgc ccgaacgcgt taattaacga
     3601 tggcttgctg caactgcgca tttttaccgg cgatgaaatt cttccggctc tcgtatcaac
     3661 cttaaaatct gacgaagata acccgaatat tatcgaaggc gcttcgtcgt ggtttgatat
     3721 acaagcccca cacgaaatca cttttaatct tgatggcgaa ccgttgagtg ggcaaaactt
     3781 ccatattgaa atacttccgg cggcgttgcg ttgtcgatta ccaccagatt gtccattatt
     3841 gcgttaatca aactattctc tcttgaacca gggagaatcc gtaatgccgg atgcggtgta
     3901 aacaccttat ccggcataca gaacaatact acaccgtaat taattttacc cgtgtcttct
     3961 gcaatgccaa tttatccacc tctgacacag catcatcggt aacaataatg tcaatttttt
     4021 cgattggcag tacctgatta aaaccgcgac gattaaattt cgatgaatcg agaacagcaa
     4081 tcactttatt tgcggcggtg accatcgccc cgctaatcga ataaccttca ttaaaggtcg
     4141 taataccatt aaccgcatca ataccatcag caccgacaaa cattaaatcg gcattaatat
     4201 cctgcaatga acgctcggca atggaaccat gcatcgagcg cgttttatgg cggaccgtgc
     4261 caccacagac gaccagagta atgtctttat tttcggaaag ggcaaacgct gccgggagac
     4321 tgttggtaat caccgtgata tttttagcgg tcattaatcc ttcggcgata agcattgtgg
     4381 tacttccgct gtcgagaata acggtcatcc cttcatggat cattttgact gccgcctgcg
     4441 ctattctgtt ttttggcgcg ctggcgagct gaaatcgctc tttaaacccg acttcctggg
     4501 tctcggtttc actattaccg gacattattt tcgccgcacc gccatgaaag cgcgtaacca
     4561 cgcctttttg ttcgagaaag cgcaaatcgg cacggattgt cgcttccgag gcagcaaata
     4621 ctcccgccag atcctgaaca agcacggttc cctgttcatt cactaattgg atgatcttat
     4681 tccttcgctc gaatgaattc atcgtcattt acccatattc aattgtggct agtgtaaacg
     4741 aagggggacc attggtgaat gatctgtttt gcacaatacc gggccagcat cacgctggcc
     4801 cgcggtttca tggaatgagc aacactttgc ccggcatggc attacgagcg atgtcacgca
     4861 ccgcctgggt gaagctttca aagcttccac ggtgagcgat taatggctcc aggcttaact
     4921 tacgttctgt cagcaaccgg ctcgccgttt cccactcctg ccccggccaa gggctggaat
     4981 agttcatcca actgccgata accgtcagct ctttacgcaa tattttgcca aacgttgccg
     5041 atgttaaatg cagatcctga tgcaacgtgc ccaccagcgc cagttgggca tgaggaccgg
     5101 caatctctac cgccagttcg acagtttgcg gtacgccagc cgtctcgagg ataagctgat
     5161 taaagcgcag ttcgcgtaaa acgctctgca tttgcggcgc gctcatttcg ctactgttaa
     5221 atgtttgcat cgcaccgaaa gattttgcca gtgccagttt ttctgagcta atgtcgatcg
     5281 ccgtcacact ctttgctccc agcgcgacag cgcactgaat agccagcagg ccaatggttc
     5341 cggcaccaat aataataacg tttttattct cacaaccttg cgctaaatga aaagcatgca
     5401 ggccaacggt aatcggctca ataaaagccc catcctcaat aggcatatcc gtgggtagag
     5461 caaagacatt ttttcgctta acgacaatat attcagcaaa tccaccatca cgccgcgagc
     5521 caataaaatc atattttgcg cactgggaat aaaacccttt caaacactct ggacaagtaa
     5581 aacagggtaa taacggcaca caggcaaccg catcgccagg gtgtaaatca tcaacaccgg
     5641 atcccaccgc atcaatatag ccgctaaatt catggcctaa cgttattgga taataatgtg
     5701 caccattttt aaatatcctg ggtaaatcgg aaccacataa gcccgagctg gcaattttta
     5761 cccgcacctc atcctgatgt ttaatttcag gaatgacgct ttttgcaacg cgcacgatac
     5821 catcagtatc attcactact gatttcataa aaactcctga ttgttaaggg ggataacctc
     5881 cccctcaggt aaaaattatt ctgcgagaac ggctttctct tgtttagtaa aaccacgcgc
     5941 tctacgccag gtcatgaaaa taccggtcag ataaattgcg ccgataataa tgaaaccggg
     6001 aatattttgc ggggagaaaa cctgaatcag taaccaggta atgggagaac cgccctgatc
     6061 cattgaagcc accatacccc cggcttttaa cgccccagca ttagccgccg gttgggtgtg
     6121 caaaccaata gtttgcgtcg cgatccacag ggtgatgctc ataatgatga cacccgagat
     6181 taaggtgcgg aacagatttc cacgatgcac cgcgaccgcc atcgccacga agaagccgat
     6241 agtggcaaga tcgccaaacg gcagtacctg attacccgga acacaaacag caattaaaat
     6301 ggtgagcgga ataaaaatca ggcttgccga taccaccgcc gtatgcccca gcagtaatgc
     6361 gggatccagg ccaatcagga actcctgacc gccgaacttc gcctgtaaac gactacgggc
     6421 ctgcttagcg atgggcgtta aaccatccat gatgggttta atcatccgtg gcatcagcag
     6481 cataactgcc gccgttttta ccgccagctg caatacacct ttgacatcgt aacccgcgag
     6541 gatgccgata atcagcccca tcacaaaacc cacggtgaca ggttcgccaa aagggccaaa
     6601 tttgcgctga atatcgtcgg cgctaaattt aattcggtta acgcctggga ttttctcgat
     6661 gatagcatcg accagcaccg caatcggccc catatacgcc gacgtaccgt gcggaatagc
     6721 aataccttcc agctcaaaga aatttcgggt atcgcgggca aaccagtcgc agagcttata
     6781 aacaaacgcc gcgtgaatta ccacacccgc catccctatc atccatgaac cggttgccag
     6841 gtgcagcaac gcgccggtga aggtcatatg ccagatattc cagatatcaa catttaccac
     6901 ccgcgtcata cgggtcagta acatcgccac gttaaccaat atcgcaatcg gaatcgccac
     6961 cagcgcaatt tgcgacgccc aggtcattgg tgaagagccc ggccagccga catcgaccac
     7021 atgcagattc aggtcgaaat tttccgccat cgctttcgcc gccggaccaa tggaatccag
     7081 cattaagcca atcacaaggc caatgccaac aaagccaatc ccgatatgca gacccgcttt
     7141 aaagcaatcg cctgccttca tgcctaatat tttagaaaaa ataatgatga caaccggcag
     7201 catcactgta gggccgaggt cgagaatata acgcatgact tctgaaaaca taggtcaccc
     7261 ctgtaagata gtcagaattt tattttgtaa tgcttcgata ccgacaccag aaacaaaagg
     7321 catgccgtga actaacggaa tatcgccaaa actacgatcc accctggcag tggtacatat
     7381 caaatgcaca ccatccatat aggtttctat ttcattaacc cgacactgga ttaattcaac
     7441 aggaatatta tgactctgac acaactcttt aatttcttcc gccgccatcg tagaggtcgc
     7501 aaccgcgcct ccgcaagcga caataatctt gcgtttcata aatagtactt tttattgtta
     7561 cagagggatt tattcgtctg aatctaaaac atactttgtg aagtattcct ttaactgagt
     7621 ttcaggaaga gtaattagtg tctcgacgat atcgggctgt tgtaacttgc caaataaaca
     7681 gcgtaaaagt ttcaattgct gctgcggatt ttccacaatc aacgcaataa ccaacgatac
     7741 cgccacgtcg ttatcatcat ccgcttgctg aaaatgaact ttatttgttg gccttaacag
     7801 ataaatggct gacgacttag catgaatcgc ctcacaatgc ggtattgcaa tggcgtgctg
     7861 ctcaagcatt atcccggtag ggaattctgc ttctctggca attaatgcct gtggccaggt
     7921 atcatgaacc acacctttcg cgagcatctc attaccgata taggttaaaa cttcgctacg
     7981 atcgacaaaa gaaattccgc tacgaacaaa caggttagtc atataccgtc cttattccgc
     8041 acagccgtag cgataggcgc gcaaaacatc ataaatttta ttcataataa gctgatgcgg
     8101 cattgctgat aattccccgg actgaatgcg ttcaaattgt ttgggaagat actgactaat
     8161 catgcccagt gggatgtcca cgccttgcag attcaccatc atcgtttcaa cgctattttt
     8221 aatccgacta tgcggccagt aataacgaat acgatccgac aggctgtaac gaatatccag
     8281 taatgaatcg ttaaaacccg tgcgataata ttttttccag tattgcggtt cgtcgagcat
     8341 cacttcttca attaccgcca ggcaaccgct gcgattttcg ggggcgataa gttcctgctc
     8401 aatttgtgcc agtgcaaata tcgcctcgcg taaagcaaag gttaatgcgg gaccgacttt
     8461 caatattgca aagtgatcgc ggactaattc ccaataagcc gtccgggtct ggtaatcggt
     8521 agaatgtgct tcataaacca ttcgggtgtt ttctatccat tgcgccagcg cctgcgcttc
     8581 ctgcggctga taatggataa tattgctgtg atcaaattcc acacccggct gcaccacgat
     8641 ggcaatcaca cgtgttaacg cctctgtcag cccacgggca ataaaggcct tttgatgcgt
     8701 acgtaaagta ttggcggcat cttcaacatg ggtgatgtgt actgactgaa tggcgctggc
     8761 ctcaccgccc ggaaccggaa cttcagtgcc aatgacatag ctcagttgct cacgctggca
     8821 atctgtcgcc acactttccg cagcaaagca aagcacagca gctcgttccg caaccgtttc
     8881 tggtgctaac ggtatggtat cccccgcgca ggacattgac gcatcaagat gaattttact
     8941 gaagccagca cgaacatatg cttttaccag ctcgacggat ttttccatcg ccgcatccgc
     9001 attttcttgc tgccagcagt ttggtcccag atgatcgccg ccgagaataa tgcgttcgcg
     9061 tgcaaaccca actttatcgg caatcgtaaa aacaaattcg cgaaagtctg ccggtgtcat
     9121 tccggtataa ccgccaaatt gattgacctg gtttgacgtt gcttcaatca gcactttgcg
     9181 cgtgctgttg cgatcaaatg ccagcgccgc ttcgataacc aacggatggg cagaacagac
     9241 tgaacatatg ccgatatgtt caccagcttt atgccgggca attaacgttt tcatgttttt
     9301 tccttgttaa atggcaggtg cgttatgccc tgccctcgca gccacaatcg gcaatcactt
     9361 tgctcaccac atcgcgcatt gcggatttag ccgactgcaa ataatcccgg ggatcggtcg
     9421 cttcggggtg ctcggtcagg taatttttta acgactgcaa gaaggcattt ttcagctccg
     9481 ttgcaacgtt gattttgcat atccccagtt tgatggtttg ctgaatatcc ttagtcgata
     9541 atcctgacgc gccatgcagc accagcggta agttcaccca ctggcgaatg ttctccagtc
     9601 gagaaaaatc aagcaccggt gcgctggcat acataccgtg agccgtgccg atggcgaccg
     9661 ccagggaatc aatcccggtt gcctcggcaa attcacgcgc ctgtgcgggg ttggtgtaca
     9721 acgcatcgac ttcattgact tgcacatcat cttcctggcc gccaagttgc cccagctccg
     9781 cctcgacgct gacatcaaag cgatggcaaa aatccaccac ctctttgacc cgtgaaatat
     9841 tttgcgcaaa aggcaaatgc gaggcgtcaa tcatgactga gcgcacgcca gaacgaacct
     9901 tctgagcgat atcgtcaaat ttcgtgtgat ggtcgagatg aattgccagt ggatggtgat
     9961 attgcttcgc catcgcgctg accagcgcca acagattttc tgtaccagca tgagtaaatg
    10021 tgccaggcgt tccggcgatg atgaccggcg catgcaggtt ggcagcggtt tccaccacca
    10081 cttgcatcgt ttcgagattg tgaatattga atgccggaac cgcataaccg ccgcgctgtg
    10141 cgttgttcag catctgcttt gtcgatacca cgtacatttt catatcttgt cgtttgcttt
    10201 cgatttcaaa atataatgaa attatttgct tttaaatgtc gagataatga tcacaaaaac
    10261 aacaatatga aaattgttcg aggagtgaaa ggcaaaaaaa cggcctcccg atagggaagc
    10321 cgtagcaaag tgcgcgtgtt tttatgccgg atgcggtgta aacatacgtg gcagtgctat
    10381 gtaggcatga taagacgctt ccgcgtcgca tcaggcatag gttgccggat gcagtgtaaa
    10441 cgtcttatcc gtcctacggg gtggtgctgt tcgtatgagg cgtatcaggc aatagtaatt
    10501 ttgctatcga gataaacgtc ctgcacggca ttaatcagtt tcacgccgtc agccatcgat
    10561 ttcttgaacg ccttacgtcc aagaatcagc cccattccgc ctgcgcgttt gttgataacc
    10621 gcagtacgca ctgcatcgct gaggtcagtt tcaccgcctg cagcaccgcc ggagtttatc
    10681 aacccagccc ggcccatata gcagttagct aactgataac gcaccagatc aatcgggttt
    10741 tcgctggtta acttgctgta cacgcgatcg tcggtataac cgtaattaat cgctttatag
    10801 ccgccgttat tttccgccat tttttgtttg acgatatctg cacctatggt tgccgccaga
    10861 tggttcgcct gaccagtcag gtcggcggaa acatggtaat caacgccatc tttcttaaag
    10921 gcggagtttc gcaaataggc ccacagcact gtcaccatgc ccagctcgtg cgcgcgttca
    10981 aaggccgcag aaatttcttc aatctggcga cgtgactctt ccgagccaaa atagatagtc
    11041 gcaccaaccg ccaccgcgcc catgttgaag gcctgctcca cgctggcata cagcgtttga
    11101 tcgtaggtat tcgggtaact tagcgtctcg ttgtgattaa gtttgacgag aaatggaatg
    11161 cgatgcgcat agcgccgcga taccgacacc aacacgccgt aagttgatgc cacacagtta
    11221 cagcccgctt cgatcgccag ttcaacaatg tttttcgggt caaagtagag cgggttagca
    11281 gcaaatgaag ctccggcaga gtgctcaacg ccctggtcaa ccggcagaat agaaagatat
    11341 cctgtgccag ccagacgccc ggtgttgtac aacgtctgca tattacgtaa caccgctggc
    11401 gggcgattat tgtcaatcat cacgcggtct acgtagtcat gtccggggag ataaagctgg
    11461 tcagaaggaa tagtcataca acggtgctgt aaaaggttgt cggcgtcttt gccaagcaac
    11521 tgcgcaatat ctgtcattac tatgctcccg taaattccga ttggatatcg gctatggatt
    11581 gtcctggccc gccttttgcg ggcaatcata atcctagtcg ttacgggcga gtttttccat
    11641 cttttggatg ttttttcagc gttttctgtt ggctcgattc atcagaaaaa atgttagcgc
    11701 ggtcaagttt tcaccgcaaa ggtattcaaa aggcattatt atgtggtatt gtcatcgcgt
    11761 accttacatt acctgtcatg aaggaattaa aagatgaaaa caacagcaaa gctgtcgttc
    11821 atgatgtttg ttgaatggtt tatctggggc gcgtggtttg tgccattgtg gttgtggtta
    11881 agtaaaagcg gttttagtgc cggagaaatt ggctggtcgt atgcctgcac agccattgcg
    11941 gcgatcctgt cgccaattct ggttggctcc atcactgacc gctttttctc ggcgcagaaa
    12001 gtgctggcgg tattgatgtt cgctggtgct gtgctgatgt atttcgctgc gcaacagacc
    12061 acttttgccg ggttctttcc gttactgctg gcctactcgc taacctatat gccgaccatt
    12121 gcgctgacta acagcatcgc ttttgccaac gtgccggatg tggagcgtga tttcccacgc
    12181 attcgtgtga tgggcactat cggctggatt gcctccggtc tggcatgtgg tttcttgccg
    12241 caaatgctgg ggtatgccga tatctcaccg actaacatcc cgctgctgat taccgccgga
    12301 agttctgctc tgctcggtgt gtttgcgttt ttcctgcccg acacgccgcc aaaaagcacc
    12361 ggcaaaatgg acattaaagt catgctcggc ctggatgcgc tgatcctgct gcgcgataaa
    12421 aacttcctcg tctttttctt ctgttcattc ctgtttgcga tgccactggc gttctattac
    12481 atctttgcca acggttatct gaccgaagtt ggcatgaaaa acgccaccgg ctggatgacg
    12541 ctcggccagt tctctgaaat cttctttatg ctggcattgc cgtttttcac taaacgcttt
    12601 ggtatcaaaa aggtattatt gcttggtctg gtcaccgctg cgatctgcta tggcttcttt
    12661 atttacggta gtgcggatga atatttcacc tacgcgttac tgttcctcgg tattttgctt
    12721 cacggcgtaa gttacgattt ttactacgtt accgcttaca tctatgtcga taaaaaagcc
    12781 cccgtgcata tgcgtaccgc tgcgcagggg ctgatcacgc tctgctgcca gggcttcggc
    12841 agtttgctcg gctatcgtct tggcggtgtg atgatggaaa agatgttcgc ttatcaggaa
    12901 ccggtaaacg gactgacttt caactggtcc gggatgtgga ctttcggcgc ggtgatgatt
    12961 gccattatcg ccgtgctgtt catgattttt ttccgcgaat ccgacaacga aattacggct
    13021 atcaaggtcg atgatcgaga tattgcgttg acacaagggg aagttaaatg aaaacagaac
    13081 gtattctcgg tgctctttat gggcaggcgt taggggatgc gatggggatg ccctccgagc
    13141 tttggccacg cagccgcgtt aaagcacact ttggctggat tgaccgtttt ctgcctggac
    13201 caaaggagaa taacgcggcc tgttatttta accgcgccga attcaccgac gatacctcga
    13261 tggcctgtgt ctggcggatg cgttactgga atgtgaaggc aagatcgatc cgaatctgat
    13321 tgggcgtaat attctcgact gggcgttgcg tttcgacgcc tttaacaaaa acgtactagg
    13381 tccgacctcg aagattgcgc ttaacgccat tcgcgacggt aaacccgttg ctgaactgga
    13441 aaacaacggc gtaaccaacg gcgcagcgat gcgcgtctcg ccattaggtt gtttgcttcc
    13501 agcgcgtgat gttgattcct ttattgatga tgtggcgctg gcgtccagcc cgacccataa
    13561 atccgatctg gcggttgcag gcgcggtagt catcgcatgg gcgatttctc gtgccattga
    13621 cggagaacgc tggtcagcga ttgttgattc attgccttca attgcgcgac atgcacagca
    13681 aaaacgcacc accacgttca gcgcctcact ggcggcacgt ctggagattg cgctgaaaat
    13741 tgtgcgtaat gccgacggca ccgaatccgc cagcgaacag ctttaccagg tcgttggcgc
    13801 aggcaccagc actattgagt ccgttccgtg cgccattgcg ctagttgaac tggcacaaac
    13861 cgacccaaac cgctgcgccg tcctgtgcgc caatcttggc ggcgacacag acaccatagg
    13921 tgctatggcg acggcgattt gcggcgcgtt gcatggcatt aacgctgtcg atcctgcgtt
    13981 aaaggcggag ctggatgcgg taaatcagct tgatttcaac cgctatgcca cagcgctggc
    14041 gaaatatcgt caacaacggg aggcggtatg agcggcgctc gattacacac actgctgcct
    14101 gaattaacca cgcgtcagcc ggtgatggtc gtcggcgcgg cggtcattga cgtgatcgcc
    14161 gacgcttatg ccctcccctg gcgtgggtgc gatatcgaac tgaaacagca gagcgttaac
    14221 gttggcggct gcgcactgaa tattgccgtg gcgttaaagc gcctcggcat cgaagcgggt
    14281 aatgccttgc cgctcggtca gggcgtgtgg gcggagatta ttcgcaaccg gatggcgaaa
    14341 gaggggttaa tcagcctgat cgataacgcc gaaagcgata acggctggtg tctggcactg
    14401 gttgagccgg atggcgaacg cacttttatg tcattcagcg gtgtggaaaa tcaatggaat
    14461 cgccaatggt tggcacgatt aaccgttgcg cctggcaacc tgctctattt ttccggttat
    14521 caactggcct cgccctgcgg cgaattgtta gtggaatggc tggaaaagct gcaagacgtg
    14581 acgccgttta tcgatttttg cccacgtatt ggcgatatcc cggacgcatt actggcgcgg
    14641 atcatggcct gtcgaccttt agtgtcactc aatcgtcaag aggctgagat tgccgccgaa
    14701 cgttttgctt tatccgcaga gataacaaca cttggcaagc aatggcagga gaaatttgcc
    14761 gcgccgttga tcgttcgtct cgataaagaa ggcgcatggt atttcagcaa cgacgcttct
    14821 ggctgcattc cggcatttcc aacgcaagtt gtagacacca ttggggcggg cgacagtcat
    14881 gcaggtggcg tacttgccgg gctggcctct ggtctgccac tggcggatgc cgtattactg
    14941 ggcaatgcag tggcgtcgtg ggttgtcggg catcggggcg gtgattgtgc gccaacgcgc
    15001 gaggaactac tcctcgcaca caaaaacgta tagatcgctg cgacagtggc taatgctgta
    15061 ctcaataggc cgttgctgtt ggtcaagcgc cacttgcttg atcaccagca ccggtatttt
    15121 gctatctaac tgaatatgtg actgaaactc ggcatccggc atccgggcgc taacgcggga
    15181 acgcgtacgt tgtgggtaaa tgtgctggct gcggaaatag tcataaagag aaattccgat
    15241 ggcatcaaca tcatgaatta aatgcgccgg aacccacgat tcctcaatcg ataccgcttc
    15301 ttcatcgaca tagcgaatac gcttaagcaa aaagacatca cttcccgctt cgaccgccag
    15361 ctgctgcgca acttcttcgg gacatttcac gacacgctta ttaacccata gcgtatcggg
    15421 ctttttcccg cacaatacca cctgctgaga aaaacctctc gcttctttca acgagtattc
    15481 gaagatgttg ttgatctgcg tgccgtaacc gcgcgaacgc gtcaccacgc cctcttcttc
    15541 cagcgcctgc atcgccttgc gcaccgtaat gcgcgaaacg ccggttaact gacttaaatc
    15601 acgctcaccg ggcaaaatat tgccatgctc cagcaccccg ctgcgcacgg cattttttac
    15661 cgtttcggca aacttaatat aaagcggcgt gttatccgcc gctaaaatac gctcgttcag
    15721 ttgggcaatc agctgggtat gcgcttgttc catttgtctt tttcctgcca gggagtctgg
    15781 tgggagtata tcactgcttc ttatttaagg cgtttcttta atcccatcaa cgaatgcctg
    15841 caactcatcc ccgtgccatt aaacacatta aaatccaccg gaccattgat gccatctacc
    15901 tgtccacggt cggaatgctg ccagaagcgc caggccatgc cgtcattgtc cggacgacgt
    15961 tgataatagt gagccaccca ccacggatat tcattgaaat agcccgccag a
//
LOCUS       DAEEUE010000087        15408 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.087, whole genome shotgun sequence.
ACCESSION   DAEEUE010000087 DAEEUE010000000
VERSION     DAEEUE010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15408
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.087"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..1173)
                     /locus_tag="KLK84_003041"
     CDS             complement(<1..1173)
                     /locus_tag="KLK84_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HBE2010283.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRL"
     gene            complement(1372..1456)
                     /locus_tag="KLK84_003042"
     tRNA            complement(1372..1456)
                     /locus_tag="KLK84_003042"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1420..1422),aa:Leu,seq:caa)
     gene            1652..2671
                     /gene="ahr"
                     /locus_tag="KLK84_003043"
     CDS             1652..2671
                     /gene="ahr"
                     /locus_tag="KLK84_003043"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HBE2010284.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2800..4302
                     /locus_tag="KLK84_003044"
     CDS             2800..4302
                     /locus_tag="KLK84_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HBE2010285.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFVQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4421..5503)
                     /gene="lptG"
                     /locus_tag="KLK84_003045"
     CDS             complement(4421..5503)
                     /gene="lptG"
                     /locus_tag="KLK84_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBE2010286.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMVIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5503..6603)
                     /gene="lptF"
                     /locus_tag="KLK84_003046"
     CDS             complement(5503..6603)
                     /gene="lptF"
                     /locus_tag="KLK84_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBE2010287.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6870..8381
                     /gene="pepA"
                     /locus_tag="KLK84_003047"
     CDS             6870..8381
                     /gene="pepA"
                     /locus_tag="KLK84_003047"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBE2010288.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8515..8958
                     /gene="holC"
                     /locus_tag="KLK84_003048"
     CDS             8515..8958
                     /gene="holC"
                     /locus_tag="KLK84_003048"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HBE2010289.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8958..11813
                     /gene="valS"
                     /locus_tag="KLK84_003049"
     CDS             8958..11813
                     /gene="valS"
                     /locus_tag="KLK84_003049"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBE2010290.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDICESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11867..13063)
                     /locus_tag="KLK84_003050"
     CDS             complement(11867..13063)
                     /locus_tag="KLK84_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HBE2010291.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICMVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLLFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13256..13759
                     /locus_tag="KLK84_003051"
     CDS             13256..13759
                     /locus_tag="KLK84_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE2010292.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13805..14221)
                     /gene="rraB"
                     /locus_tag="KLK84_003052"
     CDS             complement(13805..14221)
                     /gene="rraB"
                     /locus_tag="KLK84_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HBE2010293.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14383..15396
                     /gene="argF"
                     /locus_tag="KLK84_003053"
     CDS             14383..15396
                     /gene="argF"
                     /locus_tag="KLK84_003053"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBE2010294.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3975 a         3932 c         3966 g         3535 t
ORIGIN      
        1 caacctacgc tccccaagat gttccgcctt atggatgtaa gctgctcgca cagagctacg
       61 ctcctggtga ctcatctgcc gctctaccgc atccctcgac cataatcccg actcaatcaa
      121 cgaactacaa gccatcgtcc tgaagccatg cccacaaacc tcgaccttcg tgtcataccc
      181 cattacgcgc aaagccttgt tcaccgtatt ctcactcatc ggcttacgtg gatcgtgatc
      241 gcccacaaaa atcagctctc gatttccgct catgcttttg atcttttcca aaatacttag
      301 ggcctgccgc gacaaaggta caaggtgtgg tgtccgcatc ttcgagccac gctgagaatg
      361 cttgactcct tccagttgct cacgctcacc tgggattgtc cacatagccg tttcaaaatc
      421 tatttctgac cagcgggcaa aacgcagctc gctcgaacgg atgaaaacca ataaagtgag
      481 ttcaaccgcc agtcgagtta acggtctgcc agaatagtga tctatgcgat gaagtaattc
      541 aggtatacgg ttcagttcaa gagctgcacg gtgctgtctt ttcgccgtag caaccgcacc
      601 tgcaatctct tgcgcggggt tgtagtcgat taagccgctc tgaacggcaa agcgcataat
      661 cgcggtagta cgctgctgta aacgggcggc aacttcgagc cgcccggatg actcgactgc
      721 tttaatgggt acaagaagat cccgcgtctt cagttccgca atgttccgct taccgatagc
      781 agtaaagaga ttatcctcca gacttttcaa aacacgagcg ctatgcgatg ccgaccactt
      841 ctgattgctg gcgtgccagt ctctggcgac tacttcaaac gttatcgcct cttgttcctg
      901 ttctacctta acagctttct tgttttcact gggatcgaca ccattagcta aaagcttacg
      961 agcctcatcc cggcgtgccc tggcatccgc caacgacact tcggggtatt tccccaatgc
     1021 cagcatcttc tctttgccgc caaagcgata acgtagccgc cagtatttgg agccgttagg
     1081 gtgaaccagc aaaaccatgc cttcaccgtc ggtcagctta taggcttttg cttcaggctt
     1141 agccgaacga accttcacat cactcagagc catgatgagt atcctttcaa gggttctgtg
     1201 tgggtacaaa cattatcgaa ccgggaaata cccgcagttg tacccgcatc agtaagttga
     1261 tgtagattga atcaggttga cttaggttga gtgaaaaagc gaggaaagcc ttgcggatac
     1321 tggattttag gcacaaaaaa agacgtccgt tgacgtctat tgatgttccg atggtgccga
     1381 aggccggact cgaaccggca cgtatttcta cggttgattt tgaatcaact gcgtctaccg
     1441 atttcgccac ttcggcactg aagaggtatg cggaaaacgt tgtggattat acctgtcgca
     1501 cgccaccatg caagcgccag cttgcaccca cctcgctaag tgctgaaatt ttcagcatta
     1561 cactcagcga tatgtaacct ttgtcacact ccaggcaccc cgccctgcca tgctctacac
     1621 ttcccaaaca acaccagaga aggccccaaa aatgtcgatg ataaaaagct atgccgcaaa
     1681 agaagcgggc ggcgaactgg aagtttatga gtacgatccc ggtgagctga agccacaaga
     1741 tgttgaagtg caggtggatt actgcgggat ttgtcattcc gatctgtcga tgatcgataa
     1801 cgaatgggga ttttcacaat atccgctggt tgccgggcat gaggtgattg ggcgcgtagt
     1861 ggcactcggg agcgccgcgc aggataaagg tttgcaggtc ggtcagcgtg tcgggattgg
     1921 ctggacggcg cgtagctgtg gtcactgcga cgcctgtatt agcggtaatc agatcaactg
     1981 cgagcaaggt gcggtgccga caattatgaa tcgcggtggc tttgccgaga agttgcgtgc
     2041 ggactggcaa tgggtgattc cactgccaga aaatattgat atcgagtccg ccgggccgct
     2101 gttgtgcggc ggtatcacgg tctttaaacc actgttgatg caccatatca ctgctaccag
     2161 ccgcgttggg gtaattggta ttggcgggct ggggcatatc gctataaaac ttctgcacgc
     2221 aatgggatgc gaggtgacgg cctttagttc taatccggcg aaagagcagg aagtactggc
     2281 gatgggtgcc gataaagtgg tgaatagccg cgatccgcag gcactgaaag ccctgtcggg
     2341 gcagtttgat ctcattatca atactgtgaa cgtcagcctc gactggcagc cttattttga
     2401 ggcgctgacc tatggcggta atttccatac ggtcggtgcg gttctcacgc cgctgtctgt
     2461 tccggccttt acgttaattg cgggcgaccg cagcatctct ggttctgcta ccggcacgcc
     2521 ttatgagctg cgaaagctga tgcgctttgc cgcccgcagc aaggttgcgc cgacaaccga
     2581 actgttcccg atgtcgaaaa ttaacgacgc cattcagcat gtacgcgatg gtaaagcgcg
     2641 ctaccgagta gtcctgaaag ccgacttctg atccctttgc cgggtgctgt cgcccggcac
     2701 ttaaatctct ttggaaggcg tctcgcaatc tgctgaattt agcggtgaag agtgcacagc
     2761 cgttgcctat actccaggca ggaaactgga ggaaatctca tgagcgaacc cctgttaatt
     2821 gcccgcacgc cggacacaga actgttttta ctgccgggaa tggctaaccg tcacgggctg
     2881 attactggcg caacggggac gggtaaaacc gttacgctgc aaaaactggc agagtcattg
     2941 tcggaaatcg gcgtgccggt gtttatggct gatgtgaaag gcgatctgac cggtatcgcg
     3001 caggcaggaa cggcgtcgga aaaactgctc acaaggctta aaaatatcgg cgtcaatgac
     3061 tggcaaccgc atgccaatcc ggtggtggtg tgggatatct ttggcgagaa aggccacacg
     3121 gtgcgggcga cggtttcgga tctggggccg ctgttgctgg cgcgactgtt gaatctcaac
     3181 gatgtgcaat ctggcgtgct gaatattatc ttccgcattg cggacgatca gggattgttg
     3241 ctgctcgact ttaaagattt gcgggcgatt acccagtaca tcggcgataa cgccaaatcc
     3301 ttccagaatc agtacggaaa tatcagtagc gcatcggttg gtgccatcca gcgcggatta
     3361 ctgtcgctgg aacagcaagg cgcagcacac ttctttggtg agccgatgct ggatatcaaa
     3421 gactggatgc gcaccgatgc caacggtaaa ggcgttatca atatcctcag cgccgagaaa
     3481 ctttatcaga tgccgaaact gtacgccgcc agcctgctgt ggatgctttc agagttgtat
     3541 gaacaattgc cggaagcggg cgatctggag aaaccaaaac tggtgttttt cttcgacgag
     3601 gcacatctgc tgtttaacga tgcaccgcag gtactgctgg ataagattga gcaggtgata
     3661 aggcttattc gctcaaaagg cgtgggcgtc tggttcgttt cgcaaaaccc gtctgatatt
     3721 ccggataatg tgttcgtcca gctcggtaat cgtgttcagc acgctttgcg ggcttttacg
     3781 ccaaaagatc agaaagcagt gaagacagcg gcgcaaacca tgcgggccaa tccggcattt
     3841 gataccgaaa aggcaatcca ggaactgggg accggcgagg cgttaatctc gtttctcgat
     3901 gcaaaaggaa gtccttctgt ggtggaacgg gcgatggtga tcgcaccttg ttcgcgaatg
     3961 gggccggtga cggaagatga gcgtaatggc ctgattaatc actccccggt gtatggcaaa
     4021 tatgaagatg aggtggaccg cgagtccgcc tatgagatgc tgcaaaaagg ctttcaggcc
     4081 agtaccgagc agcaaaataa tccccccgtg aaagggaaag aggtggcggt ggatgacggt
     4141 attcttggtg gattgaagga tattttgttt ggcactaccg gaccacgcgg cgggaagaaa
     4201 gatggtgtgg tgcaaacaat ggcgaaaagc gccgctcgcc aggtgacgaa tcagattgta
     4261 cgcgggatgt tggggagttt gctggggggg agaaaaagat aagtcagacg tgatctgtgc
     4321 ccctcaccct aaccctctcc ccaaaggggc gaggggaccg ttcgaactcg tatttgactt
     4381 tgtcaacaat aataaaggga gctttcgctc ccttgttaga ttacgatttt ctcattaaca
     4441 gccacaggct gattaagaag aagctggcac ttggcaacag tgcgccgatg atcggcggga
     4501 tgccgtaaac caacgtcagc gggccgaaga tctggtccag tacgtagaag acaaaaccga
     4561 agctgatacc tgtgaccaca cgcacgccca tcggtacgct acgcagtggg ccaaagataa
     4621 acgacagcgc catcagcatc atcaccgcca cggatagcgg ctggaagatt ttgctccaca
     4681 tgttgagctg ataacgtccg gcatcctgac cgctcgactt cagatacttc acgtagttgt
     4741 gcaaaccact gatggagagt gcatccgggt ccagcgccac cacgcccagt ttgtctggcg
     4801 tgaggtcggt tttccaggtg ccgctcaccg tctgcgaacc ggtaatctgt ttcggattgg
     4861 tcagatcaga ttcatcaacc tgcgacagac gccagacttt atgttccggg tcaaacttcg
     4921 cggtagcggc ataacgtacg gattgcagac gacgattctc gttaaaggca taaatactga
     4981 tgccacctaa cacctcgtca cctttaaccc gctcaatgta gacgaagttg ttgccatctt
     5041 tcgcccataa accttgctgg gtagagagca acgagccgcc gtacatcgcc tgcgcacggt
     5101 agttacgcgc catctgttcg ccctgcggcg cgacccattc gccaatcacc atcgtcagca
     5161 agaccagtgg aatggcggtt ttcatcaccg acagcgctac ctgcatacgg gtaaaaccag
     5221 aagcctgcat caccaccagt tcgctgcgct gcgccagcat cccaagacca agcaacgccc
     5281 caagaagagc cgccatcggg aagaagatct gcacatcttt cggcacgctc agcaaggtat
     5341 acattcctgc gcctaacgcg tcgtaactcc cctgcccggc ttttttcagc tgatcgacaa
     5401 atttgataat gcccgacagc gacaccagca tgaacagcgt catcatgatg gtggtgaaaa
     5461 tagttttacc gatatagcgg tcaagtacgc caaaaggttg catcacaccg ctcctttacg
     5521 cgaaaaactg gcgcgcaggc ggcggaccgg cacggtgtcc caaaggttga gaacaatcgc
     5581 caaagccaga taaatcaggt taacggtcca catccacagt gtcgggtcca gcttaccttt
     5641 accgccgttc gatttcaggg aggtctggat caggaagaaa agtagataca gcagcatggc
     5701 tggcagcatc gacagtacgc gtccctgacg cgggttaacc acgctcaacg gtacaaccat
     5761 aagtgccatc ataaacacgg tgaataccaa cgtgatacgc cagttcagtt ctgcgcgagc
     5821 acgatcggtg tcagtgttcc acaatgtgcg catgtccatc tggtcggtat cgttcgggtc
     5881 gagcgccacc gcctggtgac caatgatcgc ctgataatcc tggaaatccg taatgcggaa
     5941 atcacgtaac agtgcagtgc cttcgaagcg cgttccctgg ttgagagtga cgacctggga
     6001 gccgtcgcgc agctgggtta aatgtccgga atcggccacc accacagaag gacgtgcatt
     6061 accttttggt cgaatttgtg cgaggaacac atctttgaaa tcgctgccgt caacgctttc
     6121 gatgaacagc accgagctgc cattagtcgc ttgctggaat tgcccttgcg ccagcgccgc
     6181 catgccaggg ttcgctttcg cttctgctaa cacttcatcc tgatgacgcg atgaccacgg
     6241 tcccgcccac atcacgttaa ccgccgcgac gattgccgtg aataccgcaa ggatcattgc
     6301 cgctttcacc agaaccgctt tgctcaagcc gcaggcatgc attaccgtaa tttcactttc
     6361 ggtatacagt ttgcccagcg tcatcagcag cccgaggaac aggcttaatg gcaggataag
     6421 ctgcgccatt tccggcacgc ccaacccgag aagggagagc accagattcg ccggaatatc
     6481 gccgtcaacc gctgcgccga ggatcctcac taacttttga cagaagaaga tcaaaagcaa
     6541 gatgaagagt atcgccagct ggcttttgag cgtctcccgc accagatatc ttatgattat
     6601 cactttaaat acgcccgtaa aaactcgtct tttgcaggat tttagcttgt ttcatggctt
     6661 aaacgtcatt tattctcttg agtcgtcgaa atcgtcgcta agataattat actcaacgga
     6721 ttcacctctc agattttgtt ctgacgtgcc aatgccgtaa taacgttaag attaacacga
     6781 agtcatcgca acagcggaca tgagttacga aagcttgcaa ttctatctgt agccaccgcc
     6841 gttgtcttta agattcagga gcgtagtgca tggagtttag tgtaaaaagc ggtagcccgg
     6901 agaaacagcg gagtgcctgc atcgtcgtgg gcgtcttcga accacgtcgc ctttctccga
     6961 ttgcagaaca gctcgataaa atcagcgatg ggtacatcag cgccctgcta cgccggggcg
     7021 aactggaagg aaaaccgggg cagacattgt tgctgcacca tgttccgaat gtactttccg
     7081 agcgaattct ccttattggt tgcggcaaag aacgtgagct ggatgagcgt cagtacaagc
     7141 aggttattca gaaaaccatt aatacgctga atgatactgg ctcgatggaa gcggtctgct
     7201 ttctgactga actgcacgtt aaaggccgta acaactactg gaaagtgcgt caggctgtcg
     7261 agacagcaaa agagacgctc tacagtttcg atcagttgaa aacgaataag agcgaaccgc
     7321 gtcgtccgct gcgtaaaatg gtgttcaacg tgccgacccg ccgtgaactg accagcggtg
     7381 agcgcgcgat ccagcacggt ctggcgattg ccgccgggat aaaagcagcg aaagatctcg
     7441 gtaatatgcc gccgaatatc tgtaacgccg cttatctcgc ctctcaggcg cgccagctgg
     7501 ctgacagcta cagcaagaat gtcatcaccc gcgttatcgg cgaacagcag atgaaagagc
     7561 tgggaatgca ctcttatctg gcggtcggtc agggttcaca aaacgaatcg ctgatgtcgg
     7621 tgattgagta caaaggcaac gcgtcggaag atgcacgccc aatcgtgctg gtgggtaaag
     7681 gtttaacctt cgactccggc ggtatctcca tcaagccttc agaaggcatg gatgagatga
     7741 agtacgatat gtgcggcgcg gcggcagttt acggcgtgat gcgtatggtc gcggagctgc
     7801 aactgccgat taacgttatt ggcgtgctgg caggctgtga aaacatgcct ggcgggcgtg
     7861 cctatcgtcc gggcgatgtg ttaaccacca tgtccggtca aaccgttgaa gtgctgaaca
     7921 ccgatgccga aggccgcctg gtactgtgcg acgtgttaac ttacgttgaa cgttttgagc
     7981 cggaagcagt gattgacgtg gcgacgctga ccggtgcctg cgtgatcgcg ctgggtcatc
     8041 atattactgg tctgatggcg aaccataatc cgctggccca tgaactgatt gccgcgtctg
     8101 aacaatccgg tgaccgcgca tggcgcttac cgctgggtga cgagtatcag gaacagctgg
     8161 agtccaattt tgccgatatg gcgaacattg gcggtcgtcc tggtggggcg attaccgcag
     8221 gttgcttcct gtcacgcttt acccgtaagt ataactgggc gcacctggat attgcaggaa
     8281 ccgcctggcg ttctggtaaa gcaaaaggcg caaccggtcg tccggtagcg ttgctggcac
     8341 agttcctgtt aaaccgcgct gggtttaacg gcgaagagta agtttgcgtt ttaccctcac
     8401 cctaaccctc tccctaaggg agagggaacc gatcgagcgc aatagaaatt cattgctcat
     8461 ctttcagggt gaggaatgca gcaatattta aatccaccac aagaagcccc atatatgaaa
     8521 aacgcaacgt tctaccttct ggacaatgac accaccgtcg atggcttaag cgccgttgag
     8581 caactggtgt gtgaaattgc cgcagaacgt tggcgcagcg gtaagcgcgt actcatcgcc
     8641 tgtgaagatg aaaagcaggc ttaccggctg gatgaagccc tgtgggcgcg tccggcagaa
     8701 agctttgttc cgcataattt agcgggagaa ggaccgcgcg gcagtgcgcc ggtggagatc
     8761 gcctggccgc aaaaacgcag cagcagcccg cgcgatatat tgattagtct gcgaacaagc
     8821 tttgcagatt ttgccaccgc tttcacagaa gtggtagact tcgttcctta tgaagattct
     8881 ctgaaacaac tggcgcgcga acgctataaa gcctaccgcg tggctggttt caacctgaat
     8941 acggcaacct ggaaataatg gaaaagacat acaacccaca agatatcgaa cagccgcttt
     9001 acgagcactg ggaaaagcag ggctacttta agcctaatgg cgatgaaagc caggaaagtt
     9061 tctgcatcat gatcccgccg ccgaacgtca ccggcagttt gcatatgggt cacgccttcc
     9121 agcaaaccat catggatacc atgatccgct atcagcgcat gcagggcaaa aacaccctgt
     9181 ggcaggtcgg tactgaccac gccgggatcg ctacccagat ggtcgttgag cgcaagattg
     9241 ccgcagaaga aggtaaaacc cgtcacgact acggccgcga agctttcatc gacaaaatct
     9301 gggaatggaa agcggaatct ggcggcacca ttacccgtca gatgcgccgt ctcggcaact
     9361 ccgtcgactg ggagcgtgaa cgcttcacca tggacgaagg cctgtccaat gcggtgaaag
     9421 aagttttcgt tcgtctgtat aaagaagacc tgatttaccg tggcaagcgc ctggtaaact
     9481 gggacccgaa actgcgtacc gctatttctg acctggaagt ggaaaaccgc gaatcgaaag
     9541 gttcgatgtg gcacatccgc tatccgctgg ctgacggcgc gaaaaccgca gacggtaaag
     9601 attatctggt ggttgcgacc actcgcccgg aaaccctgct gggcgatacc ggtgtagctg
     9661 ttaacccgga agatccgcgt tacaaagatc tgattggcaa atatgtcatt ctgccgctgg
     9721 ttaaccgtcg tattccgatc gttggcgacg aacacgccga catggaaaaa ggcaccggct
     9781 gcgtgaagat caccccggcg cacgacttta acgactatga agtcggtaaa cgtcacgccc
     9841 tgccgatgat caacatcctg acctttgacg gtgatatctg tgaaagcgcc caggtgttcg
     9901 ataccaaagg taacgaatct gacgtttatt ccagcgagat cccggcagag ttccagaaac
     9961 tcgagcgttt tgctgcacgt aaagcagtcg ttgctgcggt tgatgcactc ggcctgctgg
    10021 aagaaattaa accgcacgac ctgaccgttc cttacggcga ccgtggcggc gtggttatcg
    10081 aaccgatgct gaccgaccag tggtacgtgc gtgccgatgt actggcgaaa ccggcagttg
    10141 aagcggttga gaacggcgac attcagttcg taccgaagca gtacgaaaac atgtacttct
    10201 cctggatgcg cgatattcag gactggtgta tctctcgtca gctgtggtgg ggtcaccgta
    10261 tcccggcatg gtatgacgaa gcgggtaatg tttatgttgg ccgcaacgaa gaagaagtgc
    10321 gcaaggaaaa taacctcggt gccaacgttg ccctgcgtca ggacgaagac gttctcgaca
    10381 cctggttctc ttctgcactg tggaccttct ctacccttgg ctggccggaa aataccgacg
    10441 ccctgcgtca gttccaccca accagcgtga tggtatctgg tttcgacatc atcttcttct
    10501 ggattgcccg catgatcatg atgaccatgc acttcatcaa agatgaaaat ggcaaaccgc
    10561 aggtgccgtt ccacactgtt tacatgaccg gtctgattcg tgacgacgaa ggtcagaaga
    10621 tgtccaaatc caagggtaac gttatcgacc cgctggatat ggttgacggt atttcgctgc
    10681 cagaactgct ggaaaaacgc accggcaata tgatgcagcc gcagctggcg gacaaaatcc
    10741 gtaagcgcac cgagaaacag ttcccgaacg gcattgagcc gcacggcacc gatgccctgc
    10801 gcttcaccct ggcggcgctg gcgtctaccg gtcgtgacat caactgggat atgaagcgtc
    10861 tggaaggcta ccgtaacttc tgtaacaagc tgtggaacgc cagccgcttt gtgctgatga
    10921 acacagaagg tcaggattgc ggcttcaacg gcggcgaaat gacgctatcg ctggcggacc
    10981 gctggattct ggcggagttc aaccagacta tcaaagcgta ccgcgaagcg ctggacagct
    11041 tccgcttcga tatcgccgca ggcattttgt atgagttcac ctggaaccag ttctgcgact
    11101 ggtatctgga gctgaccaag ccggtaatga acggtggaac agaagccgaa ctgcgcggta
    11161 ctcgccatac gctggtgact gtactggaag gtctgctgcg cctcgcgcat ccgatcattc
    11221 cgttcatcac cgaaaccatc tggcagcgtg tgaaagtact ttgcggtatc actgccgaca
    11281 ccatcatgct gcagccgttc ccgcagtatg atgcatctca ggttgatgaa gccgcactgg
    11341 ccgacaccga gtggctgaaa caggcgatcg ttgctgtacg taacatccgt gcagaaatga
    11401 acatcgcgcc gggcaaaccg ctggagctgc tgctgcgtgg ttgcagcgcg gatgcagaac
    11461 gtcgcgtaaa tgaaaaccgt ggcttcctgc aaacgctggc gcgtctggaa agcatcaccg
    11521 tgctgcctgc cgatgacaaa ggtccggttt ccgttaccaa gatcatcgac ggcgcagagc
    11581 tgctgatccc gatggctggc ctcatcaaca aagaagatga gctggcgcgc ctggcgaaag
    11641 aagtggcgaa gatcgaaggt gaaatcagcc gcatcgagaa caagctggca aatgaaggct
    11701 ttgtcgcccg cgcaccggaa gcggttatcg cgaaagagcg tgagaagctg gaaggttatg
    11761 cagaagcgaa agccaagctg attgaacagc aggctgttat cgccgcactg taatcaacac
    11821 agtgatgaaa acgaaggccg gagcatgctc cggccttttt tatctcttac agaaatggta
    11881 aagaaggcat aactccccga gaaatactgg ctaagaagcc tttatctggt tgttcttctt
    11941 tattgattaa aggaaggtca tccaaatcgc ccagcaaata cgtatttttt gcctgccagt
    12001 caatcatcca tattttcagc agtgggtaag ccagaccacc cgtaatcccg gatatcacca
    12061 ccaacgcaca catgcgataa agcataccgt ggtacgttaa agttgagcga aaacgaatac
    12121 gcccatcatt cagtgacagg ttgctcataa aatgatttcg caacgacacc gttaaataac
    12181 tggtgctaac agcaatccca aaataataaa ttaactgcgc gattatcatt ttacgttgca
    12241 tttccataaa ttgctgtaaa ttctcaatat catcatttgc atacccagaa ctatcatact
    12301 catttaatat ttgatcgaag ataatataac cagcaacagc gagaaaaagt aataaagcta
    12361 aaaatgcgag gatggcatat tttatacaat acgcagtatc taatttcacc ttgaaacgat
    12421 gtataccgaa actggtattg ctccagagaa aactcattac caggctatat aaagtaccat
    12481 taaaaatacc aatggaaaca ataccaacta ttgccatcag aaccacagat ataataacac
    12541 tacttgaact attggcatgt agcatctttg tcgagataaa gaaaacagtc cccatcccaa
    12601 tggccattaa aatcggcaag aaaaaggtta cccaccagaa ccctttcaaa gagcaattaa
    12661 aactaaatct tacgccgttg agactggtca ttaaggcctg ataacgcagt ccttttgctg
    12721 ccataaatat aagcaaaacc aacagtaaca aagtcaaaac acagccaaca agcggcatat
    12781 ctgctgacac tgtcattaag attgcgaaat agaaaaaaac aaaaacaaga caactaacaa
    12841 aaacattccc accggtaatt ccataagaaa atcgttgtcc gttaacttcc atattagcat
    12901 aaagataacg cttacatttc attaatgccc atggtaaata gatacctagc gttataatcg
    12961 ttaacaacac attcaccata caaataagaa aatatctctc acctgtacca tgaaaaacaa
    13021 aagaatggga cggaacatcc atttcattaa taacttgagc cataaaaatc ccctgaatta
    13081 aaaaatccat tgatataatt tatagataca gacagtaaaa tgttctgcca cgttacaata
    13141 ctcttatcag taataggctg gcaaaaactt tattgcttta taagacgctt gcgcacgtaa
    13201 gaagagagtg gtattaaaca aatgtataat tactttattt ttattgggtt aaactatgaa
    13261 taacattgcg ccgcaatcac ctgtaatgcg tcgcctgacg ctgcaggata atcctgctat
    13321 cgccagcgtc atccgtcagg tttacgcaga gtatggtctt accgccgata aaggctacac
    13381 cgttgccgat ccgaatctcg acgaactgta tcaagtgtac agccagcctg gccatgctta
    13441 ttgggtggtt gaatacaatg gcgaagtggt cggcggcggc gggattgcgc cattaacagg
    13501 tagtgaagcg gacatttgcg aactgcagaa aatgtacttt cttcccgcta ttcgcggtaa
    13561 agggctagcg aaaaaactgg ccttaatggc gatggagcag gcacgagaga tgggcttcaa
    13621 acgctgctat ctggaaacga ccgctttttt aaaggaagcc attgcgcttt atgagcattt
    13681 gggctttgag catatcgact atgcgcttgg ctgcacgggc catgtcgatt gtgaagtgcg
    13741 gatgctacgc gaactctaaa aaaatgcccg ctagtatgtg ctatcgggca ttttaatcaa
    13801 ttaattagtg gcgaaccccg tcatcgtctt catcgacaaa atcttcatcg tcgccatctt
    13861 cgccgttagg atcttcaaag taagtgcccc aaccgtcgta ctcaacgtca aatttctccg
    13921 ccagcgtcat cagttgttca acctgggcat cgatcagatc ggcattcaac gcgcactcgc
    13981 tgaggatgtc gcagcaaatc acgatatcac catcttcaac ttccagctct tctggatcgg
    14041 tcacttcgta accgagttta aacgcttcaa ctgccgcttt ttccagagtt tccagatcgt
    14101 ctgcggaaag atggtgttcg atggtgtaca gcgcgtccgg atcgctgcca tcttccagta
    14161 attcttcaat aatcaaacgt gtttcttcac gctgttcttc cagttgttcc gggtttgcca
    14221 tggctcattc ctcttaatgt cctgccgata ctcttattgt cacacaccac tgacattgcc
    14281 tccacctttg tgacaaagat ttatgcttta gacttgcaaa tgaataataa tacacataaa
    14341 ttgaatttta attcattgag gcgttagcca caggagggat ctatgtccgg gttttatcat
    14401 aagcatttcc tgaaattact cgatttcacg ccagctgaac tcaacagcct gctgcagtta
    14461 gccgcgaagc tgaaagctga taagaaaagc ggtaaagaag aagccaaact cactggtaaa
    14521 aacatcgcgc tcatcttcga aaaagactcg actcgtaccc gatgctcttt cgaagttgcc
    14581 gcatatgacc agggggctcg cgttacttat cttggcccaa gcggtagtca gattggtcat
    14641 aaagagtcga ttaaagacac tgcccgcgtg cttggtcgca tgtatgacgg tattcagtat
    14701 cgcggctatg gtcaggagat tgtcgaaaca ctggcggaat acgctggcgt gccggtatgg
    14761 aacggcctga ccaatgagtt tcaccccacg cagttgctgg cggatctcct caccatgcag
    14821 gagcatttac ccggtaaagc attcaacgaa atgacgctgg tctatgcagg tgacgcacgt
    14881 aacaacatgg gcaattcgat gctcgaagct gcggcgctta ccggtctgga tttgcgtctg
    14941 gtcgcgccac aggcatgctg gccagaagct gcgctggtta cggaatgccg cgccctggca
    15001 caacaaaatg gcgggaatat tacgctgacg gaagatgtag ctaaaggagt tgaaggtgct
    15061 gactttatct ataccgatgt atgggtgtcg atgggtgaag caaaagaaaa atgggctgag
    15121 cgcattgcat tactgcgtga ttatcaggtg aacagcaaga tgatgcagtt gaccggtaac
    15181 ccggaggtca aattcctcca ttgcctgccc gcgtttcatg acgaccaaac gacgcttggc
    15241 aaaaaaatgg cggaagagtt tggcctacat ggcggaatgg aagtgactga tgaggtcttc
    15301 gaatctgccg ccagcattgt atttgatcag gcggaaaacc gcatgcatac catcaaagcg
    15361 gtgatggtcg cgacgctcag taaattaaat aattaatatc tctataaa
//
LOCUS       DAEEUE010000088        15350 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.088, whole genome shotgun sequence.
ACCESSION   DAEEUE010000088 DAEEUE010000000
VERSION     DAEEUE010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15350
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.088"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..157
                     /locus_tag="KLK84_003054"
     CDS             <1..157
                     /locus_tag="KLK84_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBE2010295.1"
                     /translation="IEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSG
                     GGVQELN"
     gene            386..976
                     /gene="uidR"
                     /locus_tag="KLK84_003055"
     CDS             386..976
                     /gene="uidR"
                     /locus_tag="KLK84_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HBE2010296.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1364..3037
                     /gene="uidA"
                     /locus_tag="KLK84_003056"
     CDS             1364..3037
                     /gene="uidA"
                     /locus_tag="KLK84_003056"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBE2010297.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATS"
     gene            3073..4098
                     /gene="uidB"
                     /locus_tag="KLK84_003057"
                     /pseudo
     CDS             3073..4098
                     /gene="uidB"
                     /locus_tag="KLK84_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4137..5402
                     /gene="uidC"
                     /locus_tag="KLK84_003058"
     CDS             4137..5402
                     /gene="uidC"
                     /locus_tag="KLK84_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HBE2010298.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5628..7136)
                     /locus_tag="KLK84_003059"
     CDS             complement(5628..7136)
                     /locus_tag="KLK84_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HBE2010299.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7237..8412)
                     /gene="manA"
                     /locus_tag="KLK84_003060"
     CDS             complement(7237..8412)
                     /gene="manA"
                     /locus_tag="KLK84_003060"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HBE2010300.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8611..10257
                     /gene="fumB"
                     /locus_tag="KLK84_003061"
     CDS             8611..10257
                     /gene="fumB"
                     /locus_tag="KLK84_003061"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBE2010301.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10400..11803
                     /gene="fumC"
                     /locus_tag="KLK84_003062"
     CDS             10400..11803
                     /gene="fumC"
                     /locus_tag="KLK84_003062"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HBE2010302.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11800..12729)
                     /gene="tus"
                     /locus_tag="KLK84_003063"
     CDS             complement(11800..12729)
                     /gene="tus"
                     /locus_tag="KLK84_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HBE2010303.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPTTLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12805..14106)
                     /gene="rstB"
                     /locus_tag="KLK84_003064"
     CDS             complement(12805..14106)
                     /gene="rstB"
                     /locus_tag="KLK84_003064"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HBE2010304.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14285..>15350)
                     /locus_tag="KLK84_003065"
     CDS             complement(14285..>15350)
                     /locus_tag="KLK84_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBE2010305.1"
                     /translation="SAGIGKMSLTIVACMLCLVMIAGLVGLAIASAIGLDASAFGHMQ
                     SSLTSDDVSKTAAVSLPRLITSLIPTNLFLDLTGARSVSVIGIVIFTILAGIALLKVK
                     KDAPAEGEKLSAGINAIQIWVMKMVRIVIALTPYGVMALMAKVFSTYQLSQFTNLLGF
                     IVACYLAVFVMFIVHSGILLLIGKNPVNYYKTVWQVLTFAFVSRSSAASIPLAISALE
                     KLGVPNTIANIAASFGSSMGQNGCAGIYPAVMVAMIAPTMGIDPFSLQFLATLLPAIA
                     LGSIGVAGVGSGGTFAALIVLSTLNFPVALVVIFIAIEPIVDMARTALNVNGSIMSGV
                     ISARLLKDRETKEQQSVTLN"
BASE COUNT         3933 a         3634 c         3930 g         3853 t
ORIGIN      
        1 aattgaacaa aaaatgttac agcacacgcc gatcagacgt ctgggccaac cgcaagatat
       61 tgctaacgcg gcgctgttcc tttgctcgcc tgcagccagc tgggtaagcg gacaaattct
      121 caccgtctcc ggtggtgggg tacaggagct caattaatac actaacgaac cggtaaacgg
      181 tcgtatgtgt tgtttaccgg gatagcgtgt gcaattcatc aacgtctctg ctaaacaact
      241 ggcagccaaa tcacggctat tggttaacca attgctgggt gaaaagtata cgaatagagt
      301 gtaccttcgc actattcaac agcaatgata ggcgctcacc tgacaacgcg gtaaactagt
      361 tattcacgct aactataatg gtttaatgat ggataacatg cagactgaag cacaaccgac
      421 acggacccgg atcctcaatg ctgccagaga gattttttca gaaaatggat ttcacagtgc
      481 ctcgatgaaa gccatctgta aatcttgcgc cattagtcct gggacgctct atcaccattt
      541 catctccaaa gaagccttga ttcaggcgat tatcttacag gaccaggaga gggcgctggc
      601 tcgtttccgg gaaccgattg aagggattca ttttgttgac tatatggtcg agtctattgt
      661 ctctctcacc catgaagcct ttgggcaacg ggcgctggtg gtcgaaatta tggcggaagg
      721 gatgcgtaac ccacaggtcg ccgccatgct taaaaataag catatgacga tcactgaatt
      781 tgttgcccag cggatgcgtg atgcccagca aaaaggcgag ataagcccag acatcaacac
      841 ggcaatgact tcacgtttac tgctggatct gacctacggt gtactggccg atatcgaagc
      901 ggaagacctg gcgcgtgaag cgtcgtttgc tcagggatta cgcgcgatga ttggcggtat
      961 attaaccgca tcctgatcct ctcatttcgc gggctggtga taactgtgcc cgcgtttcat
     1021 atcgtaattt ctctgtgcaa aaattatcct ccccgtgttc ggagaattcc cccccaaaat
     1081 attcaccgta gccatatgtc atgagagttt atcgttccca atactctcga acgaacgttc
     1141 gattgcttat tttatggctt ctgtcaacgc tgttttaaag attaatgcga tccatatcac
     1201 gctgtgggta ttgcagtttt tggttttttg atcgcggtgt cagttctttt tatttccatt
     1261 tctcttccat gggtttctca cagataactg tgtgcaacac agaattggtt aactaatcag
     1321 attaaaggtt gaccagtatt tttatcttaa tgaggagtcc cttatgttac gtcctgtaga
     1381 aaccccaacc cgtgaaatca aaaaactcga cggcctgtgg gcattcagtc tggatcgcga
     1441 aaactgtgga attgatcagc gttggtgcga aagcgcgtta caagaaagcc gggcaattgc
     1501 agtgccaggc agttttaacg atcagttcgc cgatgcagat attcgtaatt atgtgggcaa
     1561 cgtctggtat cagcgcgaag tctttatacc gaaaggttgg gcaggccagc gtatcgtgct
     1621 gcgtttcgat gcggtcactc attacggcaa agtgtgggtc aataatcagg aagtgatgga
     1681 gcatcagggc ggctatacgc catttgaagc cgatgtcacg ccgtatgtta ttgccgggaa
     1741 aagtgtacgt atcaccgttt gtgtgaacaa cgaactgaac tggcagacta tcccgccggg
     1801 aatggtgatt accgacgaaa acggcaagaa aaagcagtct tacttccatg atttctttaa
     1861 ctatgccggg atccatcgca gcgtaatgct ctacaccacg ccgaacacct gggtggacga
     1921 tatcaccgtg gtgacgcatg tcgcgcaaga ctgtaaccac gcgtctgttg actggcaggt
     1981 ggtggccaat ggtgatatca gcgttgaact gcgtgatgcg gatcaacagg tggttgcaac
     2041 tggacaaggc accagcggga ctttgcaagt ggtgaatccg cacctctggc aaccgggtga
     2101 aggttatctc tatgaactgt gcgtcacagc caaaagccag acagagtgtg atatctaccc
     2161 gctgcgcgtc ggcatccggt cagtggcagt gaagggcgaa cagttcctga ttaaccacaa
     2221 accgttctac tttactggct ttggtcgtca tgaagatgcg gatttgcgag gcaaaggatt
     2281 cgataacgtg ctgatggtgc acgatcacgc attaatggac tggattgggg ccaactccta
     2341 ccgtacctcg cattaccctt acgctgaaga gatgctcgac tgggcagatg aacatggcat
     2401 cgtggtgatt gatgaaactg cagctgtcgg ctttaacctc tctttaggca ttggtttcga
     2461 agcgggcaac aagccgaaag aactgtacag cgaagaggct gtcaacgggg aaactcagca
     2521 ggcgcactta caggcgatta aagagctgat agcgcgtgac aaaaaccacc caagcgtggt
     2581 gatgtggagt attgccaacg aaccggatac ccgtccgcaa ggtgcacggg aatatttcgc
     2641 gccactggcg gaagcaacgc gtaaactcga cccgacgcgt ccgatcacct gcgtcaatgt
     2701 aatgttctgc gacgctcaca ccgataccat cagcgatctc tttgatgtgc tgtgcctgaa
     2761 ccgttattac ggttggtatg tccaaagcgg cgatttggaa acggcagaga aggtactgga
     2821 aaaagaactg ctggcctggc aggagaaact gcatcagccg attatcatca ccgaatacgg
     2881 cgtggatacg ttagccggcc tgcactcaat gtacaccgac atgtggagtg aagagtatca
     2941 gtgtgcatgg ctggatatgt atcaccgcgt ctttgatcgc gtcagcgccg tcgtcggtga
     3001 acaggtatgg aatttcgccg attttgcgac ctcgtaaggc atattgcgcg ttggcggtaa
     3061 caattgacct acatgggcct cgggctttgc tacagcctgg tgaatattcc ttatggttca
     3121 cttgctaccg cgatgaccca acaaccacaa tctcgcgccc gtctgggcgc ggctcgtggg
     3181 attgccgctt cattgacctt tgtatgcctg gcatttctga taggaccgag cattaagaac
     3241 tccagcccgg aagagatggt gtcggtatac catttctgga cgattgtgct ggtgattgcc
     3301 ggaatggtgc tttacttcat ctgcttcaaa tcgacgcgtg aaaatgtggt acgtatcgtg
     3361 gcgcagccgt cattgaagat cagtttgcaa accctgaaac ggaatcgccc gctgtttatg
     3421 ttgtgcatcg gtgcgctgtg tgtgctgatt tcgaccttcg cggtcagcgc ctcgtcgttg
     3481 ttctacgtgc gctatgtgtt aaatgatacc gggctgttca ctgtgctggt actggtgcaa
     3541 aacctggttg gtactgtggc atcggcaccg cttgtgccgg ggatggtcgc gaggatcggt
     3601 aaaaagaata ccttcctgat tggcgctttg ctgggaacct gcggttatct gctattcttc
     3661 tgggtttccg tctggtcact gccggtggcg ttggttgcgt tggccatcgc ttcaattggt
     3721 cagggcgtta ctatgactgt gatgtgggca ctggaagctg ataccgtaga atacggtgaa
     3781 tacctgaccg gcgtgcgaat tgaagggctc acctattcac tattctcatt tacccgtaaa
     3841 tgcggtcagg caatcggagg ttcaattcct gcctttattt tgggattaag cggatatatc
     3901 gccaatcagg tgtaaacgcc ggaagttatt atgggcatcc gcacatcaat tgccttagta
     3961 ccttgcggat ttatgctact ggcattcgtt attatctggt tttatccgct cacggataaa
     4021 aaattcaaag aaatcgtggt tgaaattgat aatcgtaaaa aagtgcagca gcaattaatc
     4081 agcgatatca ctaattaata ttcaataaaa ataatcagaa catcaaaggt gcaactatga
     4141 gaaaaatagt ggccatggcc gttatttgcc tgacggctgc ctctggcctt acctctgctt
     4201 acgcggcgca actggctgac gatgaagcgg gactacgcat cagactgaaa aacgaattgc
     4261 gcagggctga taagcccagt gctggcgcgg gaagagatat ttacgcatgg gtacagggag
     4321 gattgctcga tttcaatagt ggttattatt ccaatattgt tggcgttgaa ggcggagcgt
     4381 attatgttta taaattaggt gctcgtgctg atatgagtac ccggtggtat cttgatggtg
     4441 ataaaagttt tggctttgcc ctgggggcag taaaaataaa acccagtgaa aatagcctgc
     4501 ttaaattagg tcgcttcggg acggattata gttatggtag cttaccttat cgtattccgt
     4561 taatggttgg cagttcgcaa cgtacattac cgacagtttc tgaaggagca ttaggttatt
     4621 gggctttaac gccaaatatt gatctgtggg gaatgtggcg ttcacgagta tttttatgga
     4681 ctgattcaac aaccggtatt cgtgatgaag gggtgtataa cagccagacg ggaaaatacg
     4741 ataaacatcg cgcacgttct tttttagccg ccagttggca tgatgatacc agtcgctatt
     4801 ctctgggggc atcggtacag aaagatgttt ccaatcagat acaaagtatt ctcgagaaaa
     4861 gcataccgct cgacccgaat tatacgttga aaggggagtt gctcggcttt tacgcgcagc
     4921 tcgaaggttt aagtcgtaat accagccagc ccaatgaaac ggcgttggtt agtggacaat
     4981 tgacctggaa tgcgccgtgg ggaagcgtat ttggcagtgg tggttatttg cgccatgcaa
     5041 tgaatggtgc cgtggtggat accgacattg gctatccctt ttcattaagt cttgatcgta
     5101 accgtgaagg aatgcagtcc tggcaattag gtgccaacta ccgtgtaact ccgcaattta
     5161 cgctgacatt tgcaccgatt gtgactcgcg gctatgaatc cagtaaacga gatgtgcgga
     5221 ttgaaggcgc gggtatctta ggtggtatga actatcgggt cagcgaaggg ccgttacaag
     5281 ggatgaattt ctttcttgct gccgataaag ggcgggaaaa gcgcgatggc agtacgctgg
     5341 gcgatcgcct gaattactgg gatgtgaaaa tgagtattca gtatgacttt atgctgaagt
     5401 aagaaataac gccggagaga aaaacctccg gcgtttcaga ttgttgacaa agtgcgcgtt
     5461 ttttatgccg gatgcggcgt aaacgcctta tccagcctac aaaaactcat aaattcaaag
     5521 tgttgcagga aaaggtaggc ctgataagcg tagcgcatca ggcaatctct ggtttgtttt
     5581 cagatgaaaa cgccggagtg aaaattctcc ggcgttttgg ccgtgaatta ctgctgcgga
     5641 attgccggta cagccggaac gttaagagct ggcatcgcaa acatgccaac aaaatcttct
     5701 aacgacattt tctgcccatt taacgttatc tgaccgttag catattgcag gctggtggtg
     5761 atggtattgt cctgcaaggt ggtcagacgg aacatctgcc ccattgctga tgcaccttca
     5821 acttgctgtt tcgccagttt tttcgcttga tcttcctgat aaccttccag cttcgctacc
     5881 tgagtcatca actcagttgc catatccacc ggaatggtca gtttcgcatc cagagattta
     5941 accgaacgat ctacttcctg cgccagcgtt tgcggcgctt ctttagtcgt tgccggatct
     6001 ttcaggaaca gcgacagatt cagcgcactt tcaccctgac tgtttttcca gcttagcggc
     6061 gcgatagtaa tcaccggatc gcctttcaac atcagcggca gggcgctaaa gaaggcttcc
     6121 gtcactttct cctgataaag ttcgggattg ttggcaattt ctggctgtgc cagcagcgcc
     6181 tgagtttgcg cgttatattg ctggctaaac tgatgccagg cttcaccatc aatctggccg
     6241 acttttaaag tcagcttgcc gctgcccaga tcctgattct gtaccttcag gctgtttagc
     6301 gagtaatcca gttggctatt gatcgtttta ccgtcattga ccagatccga tttaccgctg
     6361 atctccatgc cttccagcag tgccagttct ttgccttcca ctgaaatggt cattttttcc
     6421 agtgacagtt tttggtttcc tacgcgctca ccaaaacttc ccagcgtgct ggaaccgtcg
     6481 gttttcagat tattaaaggt caactgcact ttctggttgt attcgttaac tgcgtctatc
     6541 cgaccacttt gcgcctctcc ggaaagggag atggctttgc cgtctctgtc agcatttaac
     6601 tggaactcgc cgccgctaaa ggcgactttt tcatcctttt gctcgtaatt cagtggattg
     6661 agcgaaatat cggaactgga atcaccgctg taaccaatgc gcgagttaat ctcaaaaggc
     6721 gtttcacctt ttgccatatc aaacagtggt ttgcttactt cgttattaac cagcgtggtt
     6781 tgaattgatg ccatcgacgg gatcaggttc agttttttaa gctgggcaag cgggaaggga
     6841 ccatgatcaa ccgattcgtt gaagatgacg ctctgaccgc ttttaatcca cggattttct
     6901 ttcccggcaa tgggtttcac caacagttgc aactggctgc tgaatacgcc gcgatgatag
     6961 ttttgataac tcacttccag gttggattca ggagctgtca gtttgagttg cgcgttcgcc
     7021 tgcgcgacca tgtcttcgag atgggtttca atcttcttgc ctgtatacca tgcgccgcct
     7081 gtccagacta cgcctagcgc aacaatgacg cctaccgcta ccagcgattt attcataatg
     7141 attatccata aaatgaaatc aggcggactg gccgcctgaa ggtgttataa gcctttaata
     7201 agcttagcaa gagatgttaa ttttttcagt aagctcttac agcttgttgt aaacacgcgc
     7261 taaacggccg tggcctttga cagtcaccgg tgattcgttg gcggcaataa acgctgattc
     7321 acccggttta agctgtaact gctgagaacc tttccacaac gttgcatcgc cttcgacgca
     7381 gaacaaaatg gcggcactct gctggctgat ggtggtttct ttatcactaa ggtcatgcag
     7441 cgagaaggca aaatcatcca caggaatcgg gaagtccagt tctgcacctt gtttcaccgg
     7501 ctgggtcaac aactggttag ccggtttggc ttcgaatttc acattggcaa ccagttccgg
     7561 aatatcaatg tatttaggcg tcagacctgc acgcagcacg ttatcggagt tcgccatcac
     7621 ttccagcgcc acgccttgca ggtaagcgtg cggtgtttca gcgaacagga acatcgcttc
     7681 gccaggattc aatttcacca cattcagtaa cagcggggag aacaggccgc tgtcttccgg
     7741 gtaaaattca gaaattaaac gaatcgtttg ccacggttca ccctgctgac tgtcgagcgc
     7801 cgattttaaa atcgccagcg cgcgggattt ttcttcaccc tgcatattca acaggttggc
     7861 gaacagttcg cttaaacgtt cggcatgtgg ctgttgtaaa aagtgagcaa tcgccggatg
     7921 tgcaccggcg accggctgga gtagggagac aatctcggaa aattcacgaa acgcgttcat
     7981 cgcaaggaaa ggcgtcagcg caaaaaccag ctccggcttg tggttaggat ctttatagtt
     8041 acgctcggcg gcatccatcg ggatacctgc ggcattttct ttggcaaaac cgatttcaga
     8101 attgtgtttg tttggatgaa cctgaatgga gagtggctgt gctgcgcata atactttgaa
     8161 caggaaaggc agttcgccaa agcgtttggc aacggcctct ccgagcagag tcgatttatc
     8221 actctcaatc acatcacgca gtgaaacgat atctccggcg gcattctgca ctcgtgaact
     8281 gcttttcgga tgtgcgccca tccacagctc ggccatcggc tggctggacg gattttccat
     8341 accataaagt tcagtcaacg ccgttttgct accccaggca tagttttgca ctgagttaat
     8401 gagtttttgc atgatcaatc cctgttttaa tgtggaaatt aatcccgcta ttaaagcaag
     8461 aatcctacgg gaagtaacct ggagccgcaa aaagtcgtac tagtctcagt ttttgttaaa
     8521 aaactgtgta ggatattgtt actcgctttt aacagggcaa cggaacaccc gcccagagca
     8581 taaccaaacc aggcagtaag tgagagaaca atgtcaaaca aaccctttca ttatcagtct
     8641 ccttttccac tcaaaaaaaa tgatactgag tattacctgc taaccagtga acacgttagc
     8701 gtatctgaat ttgaagggca ggagattttg aaagtcgcac ccgaagcgtt aactctgttg
     8761 gcgcgtcagg cgtttcatga tgcgtcgttt atgctgcgtc cggctcacca acaacaggtg
     8821 gccgacattc tgcgtgaccc ggaggccagc gaaaatgata aatatgtggc gctgcaattc
     8881 ctgcgtaact ccgacatcgc ggcgaaaggc gttctgccaa cctgtcagga taccggcacc
     8941 gcgattattg ttggtaaaaa agggcagcgt gtatggaccg gtggtggtga tgaagcggcg
     9001 ctggcgcgcg gtgtctataa cacttatatc gaagataatc tgcgctactc gcaaaacgcg
     9061 ccgctggata tgtataaaga agtgaatacc ggcaccaatc tgccagcgca gatcgatctt
     9121 tatgccgttg atggcgacga atacaaattc ctctgtatcg caaaaggtgg tggttcggca
     9181 aacaagacgt atctctatca ggaaaccaaa gcgttactga cgccggggaa actgaaaaat
     9241 tacctggttg agaagatgcg cacgctgggt accgcggcct gtcctccgta tcatattgcg
     9301 ttcgttattg gtggaacttc tgcagaaacg aaccttaaaa cggtgaaact ggcttccgcg
     9361 aaatactatg atgaactgcc aacggaaggg aatgagcacg gtcaggcgtt ccgcgatgtg
     9421 gaactggaaa aagaattact gatcgaagcg caaaatctcg gtctgggtgc gcagtttggt
     9481 ggtaaatact tcgctcacga catccgcgtg attcgcctgc cacgtcacgg cgcatcctgt
     9541 ccggttggta tgggtgtttc ctgctctgct gaccgtaata tcaaagcgaa gatcaaccgt
     9601 caggggatct ggatcgaaaa actggaacat aatccaggta aatatatccc tgaagagctg
     9661 cgtaaagcgg gagaaggcga agcggtgcgc gttgacctta accgtccgat gaaagagatc
     9721 ctcgcacagt tgtcgcagta tcccgtttct acacgcttat cgcttaacgg cacgattatc
     9781 gttggtcgtg atattgctca cgccaaactg aaagagcgga tggataacgg tgaagggctg
     9841 ccgcaataca ttaaagatca tccgatttac tacgcgggtc cggccaaaac gccggaaggt
     9901 tatgcctccg gttctcttgg cccaacgacc gccggacgga tggattctta tgtcgatcaa
     9961 ctgcaagcgc agggcggaag tatgatcatg ctggcgaaag gcaaccgcag ccagcaggtg
    10021 acggatgcct gtaaaaaaca cggcggcttc taccttggca gtatcggtgg tccggccgct
    10081 gtattggcgc agggaagtat taagagcctg gaatgtgttg aatatccgga actgggaatg
    10141 gaagccatct ggaaaattga agtggaagat ttcccggcgt ttatccttgt ggatgataaa
    10201 ggaaatgact tcttccagca gatacaactc acacagtgca ctcgctgtgt gaaataaaca
    10261 gagccgccct tcggggcggt ttttttacat ggcacgaaag accaaacatt tgttatcaaa
    10321 tagtaaataa tgagtgagct aaaagttgct taacgaaagc aaaacagaaa gaaaaaatta
    10381 atcaggtgag gagcaggcca tgaatacagt acgcagcgaa aaagattcga tgggggcgat
    10441 tgatgtcccg gcagataagc tgtggggcgc acaaactcaa cgctcgctgg agcatttccg
    10501 catttcgacg gagaaaatgc ccacctcact gattcatgcg ctggcgctaa ccaagcgcgc
    10561 agcggcaaaa gttaatgaag atttagactt gttgtctgaa gagaaagcga gcgccattcg
    10621 gcaggcggcg gatgaagtac tggcaggaca gcatgacgac gaattcccgc tggctatctg
    10681 gcagaccggc tccggcacgc aaagtaacat gaacatgaac gaagtgctgg ctaaccgggc
    10741 cagtgaatta ctcggcggtg tgcgcgggat ggaacgtaaa gttcacccta acgacgacgt
    10801 gaacaaaagc caaagttcca acgatgtctt tccgacggcg atgcacgttg cggcgctgct
    10861 ggcactgcgc aagcaactca ttccgcagct taaaaccctg acacagacgc tgagtgaaaa
    10921 atcgcgtgca tttgccgata tcgtcaaaat cggtcgaacc cacttgcagg acgccacgcc
    10981 gctaacacta gggcaggaga tttccggctg ggtagcgatg ctcgagcata atctcaaaca
    11041 tatcgaatac agcctgcctc acgtagcgga actggctctt ggcggtacag cggtgggtac
    11101 tggactaaat acccatccgg agtatgcgcg tcgcgtagca gatgaactgg cagtcattac
    11161 ctgtgcaccg tttgttaccg cgccgaacaa atttgaagcg cttgcgacct gtgatgccct
    11221 ggttcaggcg cacggcgcgt tgaaagggtt ggctgcgtca ctgatgaaaa tcgccaatga
    11281 tgtccgctgg ctggcctctg gcccgcgctg cggaattggt gaaatctcaa tcccggaaaa
    11341 tgagccgggc agctcaatca tgccggggaa agtgaaccca acccagtgtg aagcattaac
    11401 catgctctgc tgtcaggtaa tggggaacga cgtggcgatc aacatgggtg gcgcttccgg
    11461 taactttgaa ctgaacgtct tccgtccgat ggtgatccat aatttcctgc aatcggtgcg
    11521 cttgctggca gatggcatgg aaagttttaa caaacactgc gcagtgggta ttgaaccgaa
    11581 tcgtgagcga atcaatcaat tactcaatga atcgctgatg ctggtgactg cgcttaacac
    11641 ccacattggt tatgacaaag ccgcggagat cgccaaaaaa gcgcataaag aagggctgac
    11701 cttaaaagct gcggcccttg cgctggggta tcttagcgaa gccgagtttg acagctgggt
    11761 acggccagaa cagatggtcg gcagtatgaa agccgggcgt taatctgcaa catacaggtg
    11821 cagccgtgga atgatcaaac gaagcggctg cgcctgaggt ttataacggt gctgcacatt
    11881 gtcggcatcg taatttaaca actcaccaac gtccggcacg cccgcgccat tatcccgatt
    11941 aatcagtgca atcagtggtg tagggcaggc gtgttggact tgtttttgat ctcctttgta
    12001 ccagacgcgg gcaatcggct gcaccttcac tggacgtttg atttttaact tcgcgttctg
    12061 tggcagggca gcgatatcct gatactctcg ctccagtttt ctttgccact cctcgcgcgt
    12121 ccacggtgcg acactgcgtg gtgatttcag gcttttttcc agctgtgcca ggacttcatc
    12181 acgatgcaaa ttcttaatga tatgtttatt agcccaacca aagcgtaaag tggtggggtc
    12241 gtgcagaacg gtgagcgtgc ggtaagcatt aagggtgatc agccccggca aatgacgatg
    12301 cacccattca aaacgtgccg cggtggggag ttctgattca accgtgacga tatgctcgaa
    12361 cgtggtcttg agtttattga tgtgctgaat atgactgacc aacgctgctt gcgaaaggtt
    12421 atcgacctgg taacacaaca cgccaggcag acggacagcg gccttgctgc tgcgattttc
    12481 ggactgttgt tgaataaata aatggcggaa atgacgcaac gccagcgact gcgcgtcgtt
    12541 gccgagatgt tgttttacct caatacgatt aagcggatta tgctcatcct ctttttttac
    12601 ctccggcaaa gagaacacgc gggcaaccaa tagcttgtgt tgctcaagat gagcggcaaa
    12661 tgtagccagc tcttgttcca tctggcgaaa ggtagtgttg agtcggtcta cgagatcgta
    12721 acgcgccata atattaacca ctttagttac aacatactta ttttatagca cagtcgtggt
    12781 ggcttgacca tcgtgcgcgt agtgtcaggc agaggtaaat tgcgggatgt tatgccataa
    12841 cggccagcta aacgagaagc gagcaccacc cagttcgctg atgtcacaat taaccgtacc
    12901 gcccattgcc agtgctattg agtggacaat tgccagcccc agcccgcagc cgccggttga
    12961 gcgatcccgg ctgggatcga ggcgaacaaa aggttcaaag atatgttcgc ggttttctgg
    13021 ggcaatccct gggccatcat cctcaacaat taatgtcgct ctattccccg acagtagcag
    13081 gctggtttca accgttgaat ggcagtagcg cagggcgtta ttgagcaaat tatccagcac
    13141 gcgctccatt aagcgcatat ccaacgccgc ataatggcct tgcatgagcg tttttatccg
    13201 taccgtttta tcgggcgtta ctgcctgaat atctgccaga tgcgttgaca gccacaacgg
    13261 caggtctggt tcgctaagat gaagctcgtt ttgtgggcga tcgagtcggg cataagtcag
    13321 cagctcttca attaaagctt caagttgact gatatcacga ttcaacgcct gggattcggc
    13381 ggcgctcagg ttatcgctca tctccagtcg ataacgcagg cgcactaacg gtgttcgcag
    13441 ttcgtgagcg ataccgtcaa taagctgttt tttgctggca attaaggcgt tgatattgtc
    13501 cgccatctgg ttaaatgcga taccaagtcg ttcaaagctc gaaccctcat caaagtggat
    13561 acgttcattg agatgcccat cgccaaatcg ttgcgccgct gcttccagtt ttaacatatc
    13621 ctgccagtgc ggacgcatcc agataaacac cggaaaggcg agggaaatag caataaaagc
    13681 gatcagggcg atatccagca atcgcatttg atggaggtaa taaagataag gaacaggacc
    13741 aactgccagc acgtagtggc tgcgcgggat acgctgcaaa aacgtgtact gatcgtccag
    13801 ggcgacaatt tcgccgccac gcagtcggtg catggaaata tcatcaagat ggtatttact
    13861 cagtggctcg acacgcagat cgaaagagag atttaaatcc atctctttca gcgtcttacc
    13921 ccagtcgtgt ggggggatct ctcgcaattc gctgcgcatc agatacagcg aactgttcat
    13981 caaatcatcc agcgactgtt tgcccgcgcg ttcggcggta aatttgtaca ccagcccaac
    14041 cagcagagac atcacaagga agcagacaaa caataacagg taaaactgga taaacaattt
    14101 tttcattggc atcgcccgca acagaatact attcgtatta attcacgact gtggagcaat
    14161 gtaaaccgcc aactggataa gaccttgttt ggaagataat gtccggaaca gacggatgta
    14221 tccccggtgt attcatattc agtaaattca attttacacc ggggcttgtt tattatgcta
    14281 aggtttaatt taacgtgacg ctttgttgtt ctttagtctc tctatcctta agaagacggg
    14341 ctgatattac accggacatt attgaaccat tcacattaag cgctgttctt gccatatcaa
    14401 cgatcggctc tatggcaata aaaataacta ctaatgcgac agggaaatta agtgttgaaa
    14461 ggacaatcag cgccgcgaat gtcccaccac tacctacgcc tgctacccct attgagccaa
    14521 gcgcgatggc aggtaaaaga gttgcgagga attgcaatga gaagggatcg atacccattg
    14581 ttggagcaat catcgccacc atcacggcag gataaatccc agcgcagcca ttttgtccca
    14641 tgctggaacc aaaagacgct gcaatattag caatcgtatt tggaaccccc aatttttcca
    14701 atgcagaaat agccagagga attgaagccg cactactgcg agaaacaaac gcaaaggtaa
    14761 gtacttgcca gacggtcttg taatagttaa cggggttttt acctattaac agcaaaatgc
    14821 ccgagtgaac aataaacatc acaaaaacgg ccagataaca ggcgacgatg aacccgagca
    14881 aattggtaaa ctgtgaaagc tgatacgttg agaagacttt cgccattagt gccattaccc
    14941 cataaggcgt cagcgcaatg acaatacgga ccattttcat tacccatatt tgaatcgcgt
    15001 tgatccccgc gctcaatttt tcgccctctg ctggtgcatc tttctttact tttaacagcg
    15061 caataccagc aagaattgta aagatgacga tgccaataac cgaaacactt cttgccccgg
    15121 tcagatcaag gaagaggttg gttgggatca atgacgtaat gagtcgcggt aaagataccg
    15181 cagctgtttt cgagacgtca tcgctggtta aagaggattg catatgacca aaggcgctgg
    15241 catcaaggcc aattgcactg gcaatagcca ggccaactaa acctgcaatc atgactaaac
    15301 acaacataca ggcaacaata gttaaagaca tcttgccaat accagcgcta
//
LOCUS       DAEEUE010000089        15345 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.089, whole genome shotgun sequence.
ACCESSION   DAEEUE010000089 DAEEUE010000000
VERSION     DAEEUE010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15345
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.089"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(174..1199)
                     /gene="tdh"
                     /locus_tag="KLK84_003066"
     CDS             complement(174..1199)
                     /gene="tdh"
                     /locus_tag="KLK84_003066"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBE2010306.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNASKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1209..2405)
                     /gene="kbl"
                     /locus_tag="KLK84_003067"
     CDS             complement(1209..2405)
                     /gene="kbl"
                     /locus_tag="KLK84_003067"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBE2010307.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2680..3537)
                     /gene="yibB"
                     /locus_tag="KLK84_003068"
     CDS             complement(2680..3537)
                     /gene="yibB"
                     /locus_tag="KLK84_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HBE2010308.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3841..4773
                     /gene="rfaD"
                     /locus_tag="KLK84_003069"
     CDS             3841..4773
                     /gene="rfaD"
                     /locus_tag="KLK84_003069"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HBE2010309.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4783..5829
                     /gene="rfaF"
                     /locus_tag="KLK84_003070"
     CDS             4783..5829
                     /gene="rfaF"
                     /locus_tag="KLK84_003070"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HBE2010310.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5833..6825
                     /gene="rfaC"
                     /locus_tag="KLK84_003071"
     CDS             5833..6825
                     /gene="rfaC"
                     /locus_tag="KLK84_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HBE2010311.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6822..8030
                     /gene="rfaL"
                     /locus_tag="KLK84_003072"
     CDS             6822..8030
                     /gene="rfaL"
                     /locus_tag="KLK84_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HBE2010312.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8066..9208)
                     /locus_tag="KLK84_003073"
     CDS             complement(8066..9208)
                     /locus_tag="KLK84_003073"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HBE2010313.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9217..10230)
                     /locus_tag="KLK84_003074"
     CDS             complement(9217..10230)
                     /locus_tag="KLK84_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBE2010314.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIVLEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10255..10962)
                     /gene="rfaY"
                     /locus_tag="KLK84_003075"
     CDS             complement(10255..10962)
                     /gene="rfaY"
                     /locus_tag="KLK84_003075"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HBE2010315.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10988..11995)
                     /gene="waaO"
                     /locus_tag="KLK84_003076"
                     /gene_synonym="rfaI"
     CDS             complement(10988..11995)
                     /gene="waaO"
                     /locus_tag="KLK84_003076"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HBE2010316.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(12038..12835)
                     /gene="rfaP"
                     /locus_tag="KLK84_003077"
     CDS             complement(12038..12835)
                     /gene="rfaP"
                     /locus_tag="KLK84_003077"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HBE2010317.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12828..13952)
                     /locus_tag="KLK84_003078"
     CDS             complement(12828..13952)
                     /locus_tag="KLK84_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBE2010318.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13949..15007)
                     /gene="rfaQ"
                     /locus_tag="KLK84_003079"
     CDS             complement(13949..15007)
                     /gene="rfaQ"
                     /locus_tag="KLK84_003079"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HBE2010319.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         4379 a         3447 c         3145 g         4374 t
ORIGIN      
        1 cctgtgagat attattttta caggtgagag agtcacttca gggtagagag ataatgagaa
       61 cagctatata aaagatcagc gcaattacga actctttatt acactgacag gattttacat
      121 aattaacaga ttatcctcag ataaaagggc tggaatacca gcccttgttc gtgttaatcc
      181 cagctcagaa taactttccc ggactggccc gaacgcatag cgtcaaagcc cttctggaaa
      241 tcatcgatag agaaacgatg ggtaatgatc ggcgagagat cgaggccaga ctgaatcagc
      301 gccgccatct tgtaccaggt ttcaaacatc tcacgaccgt aaatgccttt aatgaacaag
      361 cctttaaaga tcactttggt ccagtcgata gacatatcag acggtggaat acccagcatc
      421 gcaatacgac cgccgtggtt catggtatcg agcatggtac gaaacgctgg cggcgcgccg
      481 gacatttcca gaccgacatc aaagccttcg gtcatgccca gttcagccat cacatcatta
      541 aggttttctt tcgcgacgtt aaccgcacgg gtgataccca ttttgcgcgc cagctcaagg
      601 cggtattcgt taacatcggt gatcaccaca ttgcgtgcac caacgtgttt cgccaccgcc
      661 gcagccataa tacctatcgg acctgcacca gaaaccagca catcttcgcc caccagatcg
      721 aacgacagcg cggtatgcac ggcgttaccg aagggatcaa aaatggaagc caggtcgtcg
      781 gagatattgt cggggatttt ggaagcgttg aacgccggaa tcaccagata ttcggcgaag
      841 cagcccggac ggttaacacc gacaccaatg gtgttgcggc acagatgggt acgaccgcca
      901 cgacagttgc gacagtgacc gcaggtgata tggccttcgc cagaaacgcg atcgccgatt
      961 ttgaagcctt tcacttcctg accaatacct accacttcac cgacatattc atggcccacg
     1021 accatcggca ccgggatggt tttttgcgac cactcatccc agttatagat gtgaacgtca
     1081 gtcccgcaga tggctgtttt acggatttta atcagcagat cgttatgccc gagttccggt
     1141 acagggacgt cggtcatcca gatgccctct tccgctttca gtttggataa cgctttcatc
     1201 tcacatcctc aggcgataac gcccagttgt ttaccaatac gcgtgaatgc ttctactgca
     1261 cgcgtaattt gctcaggggt atgcgccgca gacatctggg tacgaatacg cgcctgacct
     1321 ttcggaacga ccggatagaa gaaaccggtg acgtaaatgc cctctttttg cagctctcgg
     1381 gcaaatttct gcgccactac cgcatcacca agcatgaccg gaataatggc gtgatcggct
     1441 cccgccaggg taaagcccgc cgccgacatt tgctcacgga actgacgcgc gttcgcccac
     1501 agacggtcac gcaactcgct acccgcttca accatctcca gcactttgat ggaagcggcg
     1561 acaattgctg gtgccagcga gttggagaac aggtacggac gagaacgctg ccgcagccac
     1621 tcaaccactt ctttgcgcgc cgcggtataa ccaccagaag ccccgcccag cgctttaccg
     1681 agcgtgccgg tgatgatgtc gacacgcccc atcacatcgc agtattcatg ggaaccacga
     1741 ccattttcac cgacaaaacc gaccgcgtgg gagtcgtcta ccatcaccag ggcatcgtat
     1801 ttatctgcca gatcgcaaac gcccttcaga ttggcaatca cgccgtccat cgagaacaca
     1861 ccatcggtgg caatcagcac atgacgcgca ccggcttcac gcgcttcttt cagacgagct
     1921 tcaagctcct gcatatcgtt gttggcatag cgatagcgtt tagctttaca cagacgcaca
     1981 ccatcaataa tagacgcgtg gttcagtgcg tcggagataa tggcgtcttc cgcgcccagc
     2041 agcgtttcaa acaggccacc gttagcatca aagcaggaag agtagagaat cgcatcttcc
     2101 atccccagga atgccgccag tttttgttca agctctttat ggctgtcctg agtgccgcaa
     2161 ataaaacgca ccgaagccat gccgaaaccg tgagaatcca ttcccgcctt tgctgccgca
     2221 atcagatccg gatgattcgc cagcccgaga tagttgttgg cacaaaagtt aatgacgtgg
     2281 cttccatcag ccacggtgat atctgcttgc tgcgcagacg taataatgcg ctcttcttta
     2341 aacaaccctt ccgcccgtgc ggtttccaga tcgttggtta actgctgata aaattctcca
     2401 cgcattgcga ttctccagac agggcaaatt ccagcacata ttacccaaac ttataggtcg
     2461 cgacgagatg atgcgttaac acttctgcaa aattcaggat aaataacgtg tatcttgatg
     2521 tctctcaaca cctacagata gtagaaaaac tgtacagttt tggctgagca taatgatgag
     2581 tgattttact gcattgatat aaaaagaaaa aatgatgcca aagcatcatt tttcctatct
     2641 cagttggcat caaaacgcta cccactaatg gagggaaacc tattttcttg aaaggaaaat
     2701 tctcagcgcc tgcatttttg cccctaaagt gttactcctg aagcagcgaa acaaatcgaa
     2761 ccattttcct ctccccagat agtttaggtt gaagagatcg gaacgtttgt aatagcacat
     2821 gacaaaaatg ccctggtcat cgtccacaat gttattgttt agtgttattt tttgactttc
     2881 cagaactaat ttatagaatt ctttccattt atgctgtgaa ccaacaattg cgcccccaat
     2941 aatatataca tgattgccaa tcatgaaatc aaaagcctgt tgttgagatg taacagtaag
     3001 cccttttttt atagtgaaaa gatgcatctt attttcatca aatgggaaat cccaaatttt
     3061 caatcctcga gttacatttg gcttacggca atagccaaaa tctatccacg caacaagagg
     3121 cgttttcact aaacccatgt tgatagcttt atttacaaaa taggccttaa ggttacatac
     3181 caaaacatac tctggtgacc agtactctgg gttttttaat tgtcgaggct caagtctatt
     3241 tgtaaatgac tcatcttttt gaattttttc gatacgactt ctgatatatc taaatttctt
     3301 ttttatatcg ataacgataa ccgttgttgg ttttccgttg cgaatcgctt caatccttgg
     3361 tttcaaatcg ggagaagtga aaataatcat ctcattttca agtgcggcca gacgctcaaa
     3421 gtaactaaaa taaacatcaa ctgaacgagc tagtttttca cggaaccctt tattagcagt
     3481 ccaatcccct ctaccaatgt caaaatatgc agtgataatt gtggtggatg atttcattac
     3541 taataagacc attaatataa aaaaagaatt atatcataaa tattaacaga atgtatactg
     3601 tatattatcc attcaaatga ttccgttatt tatcatacat cggaatattg atactaaagc
     3661 actattcaca tgcaaaacca acatccgcca tgaaggacta gctaaaaccc aaactagttt
     3721 gttgcaatta gcatccttgc acctctatgt aaagggctga agggattcgg atgtgatggt
     3781 atgattacag acattcgtgt ctgagattgt ctctgactcc ataattcgaa ggttacagtt
     3841 atgatcatcg ttaccggcgg cgcgggcttt atcggcagca acatcgttaa ggccctgaat
     3901 gataaaggca tcaccgatat tctggtggtg gacaacctga aagacggcac caagtttgtg
     3961 aacctggtgg atctggatat cgcggactat atggataagg aagacttcct gatccagatt
     4021 atggctggcg aagagttcgg tgatgtcgaa gcgattttcc acgaaggcgc atgctcttcc
     4081 accaccgagt gggatggcaa gtatatgatg gataacaact atcaatactc caaagagctg
     4141 ctgcactact gtctggagcg cgaaatcccg ttcctgtatg cctcttccgc agccacctac
     4201 ggcggacgca cctccgactt tattgaatcc cgcgagtacg aaaaaccgtt gaatgtctac
     4261 ggttactcaa aattcctgtt tgatgaatat gttcgtcaaa tcctgccaga agcgaactcg
     4321 cagattgttg gcttccgcta tttcaacgtt tatggaccgc gtgaaggcca taaaggcagc
     4381 atggcgagcg tcgctttcca tctcaacacc cagcttaata acggtgaatc accgaagctg
     4441 ttcgaaggta gcgagaactt caaacgtgac ttcgtctatg tgggcgacgt ggcagatgta
     4501 aacctgtggt tcctggaaaa tggcgtttcc ggtatcttca acctcggcac cggtcgtgca
     4561 gaatccttcc aggcagtagc agatgctacg ctggcttatc acaagaaagg ccagatcgaa
     4621 tacattccgt tcccggataa gctgaaaggc cgctaccagg cgttcactca ggcagatctg
     4681 acaaatctgc gcgcggcggg ttacgacaaa ccgttcaaaa ccgttgctga aggtgtaacg
     4741 gaatacatgg cctggctgaa tcgcgacgca taagagctct gcatgaaaat actggtgatc
     4801 ggcccgtctt gggttggcga catgatgatg tcgcaaagtc tctatcgcac gctccaggcg
     4861 cgctatcccc aggcgataat cgatgtgatg gcaccggcat ggtgccgtcc attattatcg
     4921 cggatgccgg aagttaacga agctatccct atgcctctcg gtcacggagc gctggaaatc
     4981 ggcgaacgcc gcaaactggg tcatagcctg cgtgaaaagc gctacgaccg cgcctacgtc
     5041 ttaccaaact ccttcaaatc tgcattagtg cctttcttcg cgggtattcc tcatcgcacc
     5101 ggctggcgcg gcgagatgcg ctacggttta ctcaacgatg tacgcgtgct cgataaagaa
     5161 gcctggccgc taatggtgga acgctatgtc gcgctggcct atgacaaagg cattatgcgt
     5221 accgcacaag atctgccgca gccattgtta tggccgcagt tgcaggtgag cgaaggtgaa
     5281 aaatcatata cctgtaatca attttcgctt tcatcagaac gtccgatgat tggcttttgc
     5341 ccgggtgcgg agtttggtcc ggcaaaacgc tggccacact accactatgc ggaactggca
     5401 aagcagctga ttgatgaagg ttatcaggtg gttctgtttg gctctgcgaa agatcatgaa
     5461 gcgggcaatg agattcttgc cgctttgaat accgagcagc aggcatggtg tcggaacctg
     5521 gcgggggaaa cacagcttga tcaagcggtt atcttgattg cagcctgtaa agccattgtc
     5581 actaacgatt ctggcctgat gcacgttgcg gcggcgctca atcgtccgct ggttgccctg
     5641 tatggtccaa gtagcccgga cttcacaccg ccgctatccc ataaagcgcg cgtgatccgt
     5701 ctgattaccg gctatcacaa agtgcgtaaa ggtgacgctg cggagggtta tcaccagagc
     5761 ttgatcgaca ttactcccca gcgcgtactg gaagaactca acgcgctatt gttacaagag
     5821 gaagcctgac ggatgcgggt tttgatcgtt aaaacatcgt cgatgggcga tgttctccat
     5881 acgttgcccg cactcactga tgcccagcag gcaatcccag ggattaagtt tgactgggtg
     5941 gtggaagaag ggttcgcaca gattccttcc tggcacgctg ccgttgagcg agttattcct
     6001 gtggcaatac gtcgctggcg taaagcctgg ttctcggccc ccataaaagc tgaacgcaaa
     6061 gcgtttcgtg aagcgctaca agcagagaac tatgacgcag ttatcgacgc tcaggggctg
     6121 gtaaaaagcg cggcactggt gacacgtctg gcgcatggcg taaagcatgg attggactgg
     6181 caaaccgctc gcgaaccttt agccagcctg ttttacaatt gtaagcatca tattgcaaaa
     6241 cagcagcacg ccgtagaacg cacccgcgaa ctgtttgcta aaagtttggg ctatagcaaa
     6301 ccgcaaaccc agggcgatta tgctatcgca cagcattttc tgacgaacct gcctacagat
     6361 gctggcgaat atgccgtatt tcttcatgcg acgacccgtg atgataaaca ctggccggaa
     6421 gaacactggc gagaattgat tggtttactg gctgattcag gaatacggat taaacttccg
     6481 tggggcgcgc cgcatgagga agaacgggcg aaacgactgg cggaaggatt tgcttatgtt
     6541 gaagtattgc cgaagatgag tctggaaggc gttgcccgcg tgctggccgg ggctaaattt
     6601 gtagtgtcgg tggatacggg gttaagccat ttaacggcgg cactggatag acccaatatc
     6661 acggtttatg gaccaaccga tccgggatta attggtgggt atgggaagaa tcaagtagag
     6721 tgtcgttcta caagtatgtc tcttgcagat ttgccagctc aaaccgtttt tcaaaattta
     6781 aatcttgaaa taataaccaa taagttgaca tcggagataa gatgacctca acattatttt
     6841 tctctctcga gaaaaaaaac tggatagcgt actggaacag agctctcgta ttcttattca
     6901 ttaccaccta ttttttgggt gggattacaa ggtataaaca tcttattgtt attcttatga
     6961 caataacgac aatcgtctat ctctgcaaac ggccaaaaca ctatctctca ctatttaaaa
     7021 catttctttt tggtagtgtt gccatattaa ctattgctgc attgctgtca cttcttcaat
     7081 cccctgatgc aggtgctagc atgaaggaag ttttcaaagc tattattgag aatactttac
     7141 tatgcacaat agcaataccg gtcatattga gagacgagaa aagagaagat gtcgaaaaaa
     7201 tcgttttctt ctcatttatt agtgcgttgg gcttacgctg tttttctgaa ttgattacct
     7261 attataagga ctatcaacaa gggataatgc cattcgcaga ttatagacac cgtagcattt
     7321 ctgactcgat ggtcttttta ttccctgcat tgttaaatct ctggcttatc aaatcagcaa
     7381 aataccgcat ttcttttgtg gttctaagcg ttatttttat ttttctgata ttaggaactt
     7441 tatccagagg ggcctggctt tccgtgttag tcattggatt aatatggatt ctgatgttta
     7501 aacaatggaa gttactatta gtaggagtaa tggtttccat cattgcattg tcggttattt
     7561 tcacacataa ggagatgacc gcaaagctaa cgtataaact tcaacaaact aatagttctt
     7621 atcgctatgc aaatggtact caaggcagcg cactcgatct aatattagaa aatcctgtta
     7681 ttggttatgg ttacggtaac gttgcatata aagatgtcta taataaacgt gtcattgatt
     7741 atccagaatg gacctttaga caatcaatag ggccacataa ttttgcgcta ttcatctggt
     7801 ttggcactgg tttattaggg ctggtaagtc ttatgatgct atactgtgca atattgaaag
     7861 agtgtataaa aaatggcgtc aagaataaat atcgctcacc atataatgca tattatataa
     7921 tcttactatc ttttataggt tattttgtta tccgtggaaa cgtagaacaa attgaaccaa
     7981 atttattagg cgtttacgcc ggcttattat tagcgatgaa aaacaagtaa aaaataaaaa
     8041 aggctgcata atgcggcctt tttatttatt caaaccaatt atgaataacc tcttcgaatc
     8101 tttgagttac gccatcccaa ctgtagtgat caaacacaaa gtcctgacct tgctttgcaa
     8161 ctgctgttaa ttcaggattc gctaacgttt ttaaaatatc gctgcttatg gagtctgcag
     8221 tcataggttc ttttaaatgg aatcccgtag tattttcttt gacaaattcc gtcatccccc
     8281 cacgcgtact cactaaaaca ggcttaccag cacccatagc ttcaattgcc accatgcaaa
     8341 atggttcctg gaactgggaa ggaatgacga ctaaatctgc cagtgggtaa taacaataca
     8401 tcttttcagg cggtatgctt cctaacataa tgcaacgatc cttcaaacgt tttgcaattt
     8461 ccctgacatt gcgttgatat gcacctttgt cacttttact catttctgta taatcaccaa
     8521 tcacaactaa ttttaaattt ttgtgagcag cggccaattt ttcgaatgat tgcatcaaca
     8581 gcaatatgcc tttgtcaggg acgatacgcc cagcataaaa aataattttc tcttcaggag
     8641 agatattaag ttcgcttcta gtgatcggct gaaaattaga ttgataggtt tctaaatcaa
     8701 ttccatttgg tacaatctca atatctgcat ttgctaaata agattggtaa tactttttca
     8761 agtataaact agggactatc attttaacat tttgctcaag caattttggt tcaaatgcat
     8821 tatgcatatg tataaccact cttgcctgag gagctagctt acgaatttga gtgtatagct
     8881 tcatgctatt atgaactaca ataacacttt ctttcgttat aggaaaatca tgtgcaatat
     8941 tcaatattct ctgtgaatat ggaagaggat caagtcttgt ccatttttga aataaccgct
     9001 tatatatgcg actgaatcca accctatgaa tactacaatt atcattaaca aaggtgtaat
     9061 ttgagtatcc tggatttttg atacaaacaa tacgatttgg aaaattggta cgctgagcaa
     9121 cctgatacat ccacgtttca acagcagccg ctccgcgagg aggaattgaa aaaataggag
     9181 taaccgtaaa tattatttta tcaaccatct cgtatattaa cctttcataa cattatattt
     9241 aattgctgta aaaacaccat cgagaaattt tttctggtaa agcaagtgtt tatatttttc
     9301 tttgtgctca tgttttttaa cagctttttt ataaggtata tttctccatg gtgatatatt
     9361 ataaatatta cgaaaataat ctgcagaggc gtagttcgcc caatcatgcc atggcttagt
     9421 tataccagta taatgtatga agacagtgtc atcattaata aaccgggtgt aatattcact
     9481 atttttttct tcaaattctg actttatagt ataaatacaa ttatattttc tcggtaatat
     9541 tttagcatga tgtagaaaca ttaagtttaa aatatcttga tccgggtact ttaatttttt
     9601 tataattgac tcatctgata atagatcaaa gaatttttcc gttagtcttt gttttaacca
     9661 ttcccgtaaa ttaatataca ttacaccaga gttaaaatag ctaccgttaa aatcctcatt
     9721 acacaaacgc tcagcgcttt tactttgcat agcatctaca tcaattacca ctgccccata
     9781 ttcatcttta aactctaata ctattaactc gttcaatgaa cccttacaga cgatatcagc
     9841 atcaagatat aataatgaat ccaatcgcgc agaaaaataa tcaaatgaaa gcaaacgata
     9901 ataaatagat actggccaga tatttgattg aggtagcgcc ttcaatggct cagaatcaat
     9961 atgatataat ttaattactg tttgatactt cgttgctaat tgtgataata attcaatata
    10021 ctctttgtct aagtaatcag cgattatgtg aaaggtaaat ttgttttgtt tattcttttc
    10081 aagaattgaa gcaattgaaa tcgcagcccc aacctggtag ttctcatcaa taccccatga
    10141 gacattaaac gtttcccgtt catcaagttt aacggggcgc ttgtccagtt caattatatc
    10201 tttaaattga gtaagatgtt taaaatccaa tttaattacc cttcatcata atcattattt
    10261 gctcttcaat cctagtttga cttttacatc cctgattact tttttaattt tctttttgaa
    10321 tataagatat gtatagccaa agtctttcaa ttcatttttt atattatagt gccgttcaag
    10381 atcaatgcga tcttttgctt ttaaaacagc ggttgttttt ttacctgaaa ggtcaattag
    10441 tcttaaacct ttttcggata ctataaaatt tcctttatga ggatcgccag acaccattcc
    10501 atgctgatgc aactctttta tcgattctga taattgatcc ttcagatctt cagaaatttc
    10561 caaatactca tttaacccga caccttcaat atactcaatg agcataatat aataatgagc
    10621 gaaatttaat gttttacgtt cagctagcaa aaaatagtca ttaattgatt ggatgccttc
    10681 accacgtacc cgatcggttt gataaattaa attttcataa taatcttttt ttatgcaaga
    10741 ttttaagaaa cgttcggtca ttttatgctt tggcgcataa actttaagta caagaggtcc
    10801 acgagcagtg tcaataagaa tgactttagt atcatcaata gaacgaaaaa cttttaatgt
    10861 tttaaccctg caagttaaaa agtcatttag aacttgctca taaaagggat cattatcttt
    10921 tatgaaaact ttcaacccat ttatcgtttt attatatatc ataatgaatt attttaacct
    10981 taaatcttta gaagcatttt tctttataat actttaaata attaaaaatg cctgccatat
    11041 aatgcttttg tttaaattta tgttttgcac aataccgata ctgattgcta tttacaggtt
    11101 taagtaaatc ttctttactc cacggagaag ctgctttggc gatcaaaaaa ctacgtgaaa
    11161 ccggataatc agcccactca tgccagggtt ttgtaggtcc cacatagtgt ataaacacgg
    11221 tgtcatcatt gactggatta tcaactttgt cttttaattc atagttaata ctatatcggg
    11281 tattatattt ctccgaaata aacttcactt taccattcaa taatacattc agtacatctt
    11341 gatcaaggtg ggtgatttta cttacccaat ctgggtcacg caacatttca atagcttttg
    11401 acgaaatgtt atttagattc cactcatcaa tatttatcag taaaaaacca gcattgaaat
    11461 atccagaagc taactgtggt gtagttaata ccgaggctcg attttgccac cattcaatat
    11521 ctctttcagc tacaactgcg gcaatttcat tagtagaaaa ttgataatct aagagttctt
    11581 taatactacc cttgcaagca atatctgcat caagatatag tattttttca tgtttatgat
    11641 agaaataatc tgcaattata aatcgaaaat atgttgcgta agtccagttt ttcgtgcttg
    11701 gtaatgactt cagcttatca caattgataa cataaatatt aattcttgaa ttatattgtt
    11761 ttgctaaatc agaaaaatat aatttgtctt tatcactgat ataatctgtg aaaacatgaa
    11821 attcacaaga gatttctcta ttgtttaata gaatagatgc gatggctaca ccacatccaa
    11881 aaagaaaatt tctgtcaata ccaaatgcta tatcaagagt atttttagat cttatatttt
    11941 gataatgaaa attatactca aaaaggatga tgtcactatc attgagttga gacatattta
    12001 ttctgtatcc aaaattttgg caaaaataag ttacgggtta caacgatttt cgaatcgttc
    12061 tttccctgat ttttgtggct tttgcttctg cttgcgacag cagtccttgt tcctgcttga
    12121 gaatgtcttt aagcggggcg gcaaaataca ccttcataaa gcgccagata tcccgctgag
    12181 tcaggccaat attcattgaa gaaaaataaa gccctataag atctttatcg cgccaacgac
    12241 ctggaacgcg cgtgcgaagc tgcgcccggt gtaggtcaat taccgaaatt tttaactcct
    12301 cttccttacc ggaaaaaggc aagtgcagca ggaaatgaca gatataacag tcacgatggt
    12361 taatgcccgc agcatgcata tcgcgcacca tcgtcgcgac acgcttaata agcatacgct
    12421 ttacgcgaac atctggtggg ttggtcgccc agtcagcaca gtaatcttcc agactgatgg
    12481 ttggtgtcag atcttcggta ataataaacg aggttctggt cagcggattc atgccttttt
    12541 cgccgaatgc caccccatac atagtatcaa cgccgacatc ccgcagtcga tgaatcgcat
    12601 tccattcgcg gtctgcgcct aatactggca tccgcaatga gagtaaattt ttgattatct
    12661 ctttcagggt cgtgccgcga tgccatttga gaaaatagct ttttcccgcc atttcaaagc
    12721 gcagagtacg gcgagtttcc agttcacgaa atacctcacc ctgcaaggtt ttaacttcct
    12781 caaaaggatc tttgccgcgc cataacgtgg caaacggctc tttaagttca accatccaga
    12841 ccacccgtta tgatatccgc cgctttctct ggcagactgt ataaatcttg tgtatcagca
    12901 taatgtcgcg cattttccgc ccaagcctgg cgcaatgaag attgcgttaa cgctttgcgt
    12961 aaaatctcat tcaatgtttc ctggcggaat ggctcagcaa tagcctcgcc gcaattagcg
    13021 tcgacaatat aatgcgcata gccacaaacg gcagttgtta gtaccggtaa tcctgcagta
    13081 atcgcttcca gcagcacaat tcccgccgct tcctggtagg caggatgcag taataaatcc
    13141 gccgccgcca ttaattccga gacatcgttg cgccccgaga agaagtgaac attactgcgc
    13201 acgccgcgtt tttctgccag tgcctcaaat tttcgcggtt tatcctgccc aacaacatat
    13261 agcaatgtgt tgtggcgcag cgaatccggt aacgaagcaa gtgcttcaat ggaacgatcg
    13321 gcacctttac gcgtgaagtc tgaaccgacc tgcaacaata aatattgttg ttcggttatt
    13381 ccattcttct tacggaagat ttcacggcta ttggctggct gctggctata tttacgatca
    13441 ggataaatcc ccggtggcag aatatgaaaa cgctccgctt cagtctgata atgtttctgg
    13501 aaatcggcga tttgcttatc tgtcagcatc agcagctgtg tcggcttgcc ctgttcgaag
    13561 gttgcccgct caaaggcggc ataatggcga taacgtgacg tcaggcgata gaaaaagcct
    13621 ttttcctgcg ctactttctc ggcataacaa acatcagcgg cataataaac gtccagcccc
    13681 ggcattttgt tgaatccaac gactttatcg acgggatgtt cgcgtaaatg tttttgcacc
    13741 caggcaaaat actccgcatt gcgcccgtga ttggtatgcg atttaaccgg cactttgatc
    13801 acttcaaata catcagggca ttcgccttcc cacgactggg tataaacccg aacatgatga
    13861 cctcgggcgg cgactgtctg agcaatacgc ataaaatcgc gctgcaaacc gccaaaggga
    13921 aaatatttat ataaacaaaa agcaacgatc ataacgacgt acctgtcgtg gaggagggca
    13981 gtaatttatc gacagcagca atgacatctg ccgccggaat aaccgaaaga tacatctcat
    14041 ttcggtcacg ctgatcgcgc gttggcattt cccggtaatc tcccgcccag aattgaatca
    14101 tgttatttga ccagggacgc cagaaaatat gatctgttgc gccaaacagc gatatcagcg
    14161 gcgtattaac tgcagcggca atatgcgccg gtgcggaatc aacgccaata aacagctgcg
    14221 catgatcgat taacgcacca agttccggga aggtcacctt tccagccagc gccgttactg
    14281 gtggcgtctg gcatccctgc gcaatttcat tgacgcaggc cagatcgtct ttatctgggc
    14341 cggacgtcag aacgacttca taaccacgag catgtaaggc atcaatcaca gcggaaaact
    14401 tggcgttgtc ccagcatttg aagatttgcc gcgccgtagg ttggataacc acataatttt
    14461 gtccaacacc agcgtgatca agttcgcgac gcatacgttt ccagcttgca ggcgggtaac
    14521 tcatggttgt ctgcttcacc aacgaatcaa gtcccaatgg tgtcagcacg gataagttac
    14581 tttccaccac atttccaccc tgcaacggca ccaaatgggt gaaactttta cgccaaaaag
    14641 cagactgccg atgatgataa tcctgggaaa ttttcacacg agcatttaat aagcgaacca
    14701 gtatagcaac catccattga tcggtaagat tgacgataag gtcatactta ttggcacgta
    14761 ataccttgat gagatgaaaa aagttggcaa ttttttctga ggcttttgct tttttatttt
    14821 ttatgccgta gagcgcgtta atctctggat tttcagacag gatcgggatg gtgtcctgat
    14881 aaagcagcac atcgattttt gcgtcagggt aatttttttt cagagaacta atgacgggag
    14941 tagttaataa catatcccca tgaaaacgca tcttaatgag caaaattctt cgaaatggct
    15001 tatccacaag taactctttt tgttgagatt atccgattaa ggtgaagcag aaaaagcacg
    15061 ctaccgcccc aggctcaaca gctacctgaa tactgatacc gcactgatat tgacgcgcta
    15121 gtgtatcact tctttcgcgg caatccgacc cgaatgattt ttaagcacat tgttgatttt
    15181 caataaagaa atcaatcaaa taaaatcatt agcaaccata ttctttcact gttgctctgc
    15241 gattccttcc tgcacgtaca gcagaaatca atgaatcgca taaaaataca aaaaaagtat
    15301 agatgttcag caagaattgg cgggtaaatt gctatttaag tcaaa
//
LOCUS       DAEEUE010000090        15099 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.090, whole genome shotgun sequence.
ACCESSION   DAEEUE010000090 DAEEUE010000000
VERSION     DAEEUE010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15099
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.090"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..179
                     /gene="yjbD"
                     /locus_tag="KLK84_003080"
     CDS             <1..179
                     /gene="yjbD"
                     /locus_tag="KLK84_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HBE2010320.1"
                     /translation="VLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEA
                     SFSWSANTSTRGRR"
     gene            complement(312..1184)
                     /gene="rluF"
                     /locus_tag="KLK84_003081"
     CDS             complement(312..1184)
                     /gene="rluF"
                     /locus_tag="KLK84_003081"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HBE2010321.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRYLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1532..2479
                     /locus_tag="KLK84_003082"
     CDS             1532..2479
                     /locus_tag="KLK84_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBE2010322.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2549..3355
                     /locus_tag="KLK84_003083"
     CDS             2549..3355
                     /locus_tag="KLK84_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBE2010323.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3365..3772
                     /locus_tag="KLK84_003084"
                     /pseudo
     CDS             3365..3772
                     /locus_tag="KLK84_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
     gene            3772..4266
                     /locus_tag="KLK84_003085"
     CDS             3772..4266
                     /locus_tag="KLK84_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HBE2010324.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4332..5129
                     /locus_tag="KLK84_003086"
     CDS             4332..5129
                     /locus_tag="KLK84_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBE2010325.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            5140..5928
                     /locus_tag="KLK84_003087"
     CDS             5140..5928
                     /locus_tag="KLK84_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBE2010326.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5980..7209
                     /gene="sorE"
                     /locus_tag="KLK84_003088"
     CDS             5980..7209
                     /gene="sorE"
                     /locus_tag="KLK84_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HBE2010327.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            7591..8280
                     /gene="pepE"
                     /locus_tag="KLK84_003089"
     CDS             7591..8280
                     /gene="pepE"
                     /locus_tag="KLK84_003089"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HBE2010328.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8371..10002)
                     /locus_tag="KLK84_003090"
     CDS             complement(8371..10002)
                     /locus_tag="KLK84_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBE2010329.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10222..13905)
                     /gene="metH"
                     /locus_tag="KLK84_003091"
     CDS             complement(10222..13905)
                     /gene="metH"
                     /locus_tag="KLK84_003091"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBE2010330.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14105..14929
                     /gene="iclR"
                     /locus_tag="KLK84_003092"
     CDS             14105..14929
                     /gene="iclR"
                     /locus_tag="KLK84_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HBE2010331.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELSQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         3591 a         3982 c         3937 g         3589 t
ORIGIN      
        1 gcgtactgac caactccgaa accaacagca ttaagaaaga gtacatcgat aaattaatgg
       61 tcgagcgtga ggctgaagcg aaaaaagccc gccaattgaa gaaaaagcag gcttataaac
      121 cggatcctga agcgtcattt agctggtcag caaatacgtc gacgcgcggc aggcgctaat
      181 gaacaatcaa cgcctgagat tttcactcgt ctgtgtggac acaatcttcc cctcacccta
      241 accctctccc cgaaggggcg aggggactgt ccgagctctt tttggacgtt ttcatcagtc
      301 tgaggcgtta atcagcgtcc cttcttttta cgccccggcg aggtaaaacg cttaccgttg
      361 gacgccgggc gaccgccttt ttccgccgtt ttttccatct taacgactgg acgtttgatg
      421 cccgctgttt tcggtttggc cttcgcttta ggttttgcct cggaagagga attttcgatg
      481 agcttaaaga ggtcgattaa ctcatcgtcg gttaaatagc gccactcccc cagcggaatg
      541 ccgcttaagc tgacgttcat aatgcgcgta cgttccagct ttttcacttc atagccgaaa
      601 tgctcgcaca tgcgccggat ctgacggttc agcccctgca ctagggtaat gcggaagaca
      661 aacggcgctt cttttttgac tttgcacttt ttcgtcaccg tcccgaggat cggcaccccc
      721 gcccccatac cacgaataaa ttcatcggta atcggtttat cgaccgtcac cagatactct
      781 ttctcatgat cgttacccgc acggaggatc ttattcacca gatcgccgtg attggtgagg
      841 aaaatcagcc cctgggagtc tttatccagg cggccaatcg ggaacacgcg tttgctgtgg
      901 ttaacgaaat cgacaatgtt atcgcgctcg ccatcttcgg tggtgctgac aataccgacg
      961 ggcttgttca gggcgataag taccaaatct tcggcttccc gaggctcaat caactgaccg
     1021 tttactttca caatgtcgcc gggtttcacc tgatcgccaa tggtggctcg cttgccatta
     1081 aggaacacat tgccttgctc gatatagcga tccgcttcgc ggcgtgagca aattccgctt
     1141 tcgctgatgt atttatttaa acggactgat gagtcgggca gcataaattc tcctgtaaaa
     1201 cgcggaatat accgtacctc ttagcgggga aaaacagggt tatgcggcaa cgtcgaaatt
     1261 aaatgtgatc tgccacgcct tttatcacaa aagtgtctcc ggaagtacga tgcaaatttg
     1321 cctggcaaaa taaggataat caacatatta cgaatcgctg cacacctgtg ctgatgacta
     1381 ctacgcagag aaacaaatgt gcaagagaaa gcgctatttc tccccatgca aagagtgaaa
     1441 aatcgctttg aaaggccctc atgatggggt tatatcagat caagcggtga gaaagcgtat
     1501 ttgcacaaat gtgcagaacc aggaggcggc aatggaaaac agtgacgata tccgtttgat
     1561 tgtgaagatt gcccaactct attacgaaca ggatatgacg caggcgcaaa tcgcgcgcga
     1621 actgggtatt taccgcacca ccatcagccg cttgcttaaa cgaggccgcg atcagggaat
     1681 ggtcaccatc gccatcaact atgactacaa cgaaaatctc tggctggagc agcaactgaa
     1741 gcaaaagttt ggcctgaaag acgttgtggt ggtgtcggga aatgatgagg atgaagagac
     1801 tcaactggcg atgatggggt tacacggcgc gcaactgctg gatcgcttgc tggagcctgg
     1861 cgatattgtc ggtttttcct ggggtcgcgc ggtgagcgca ctggttgaaa acttgccgca
     1921 ggcggggcaa tcgcggcagt taatctgcgt accaattatt ggcggaccgt ccggtaaact
     1981 cgaaagccgc tatcatgtaa acacattaac ctacagcgcg gcagcgaagc tgaaagggga
     2041 atcgcatctc gcggattttc cggctctgct ggataaccca ttaattcgta atgggatcat
     2101 gcagtctcag cactttaaaa ccatctctgc ctactgggat aatctggata tcgccctggt
     2161 gggaattggc tcaccggcca ttcgcgacgg cgctaactgg catgcgtttt atggtggtga
     2221 agagagtgac gacctgaatg cccgccaggt tgctggcgat atttgctcgc gcttttttga
     2281 tattcacggc gcaatggttg aaacgaatat gagcgaaaaa acgctctcta tcgaaatgaa
     2341 taaattaaag caggcgcgat attccattgg cattgccatg agtgaagaaa aatacagcgg
     2401 aattattggt gcactgcgtg gaaaatatat taattgtctg gtaacgaata gcagcacagc
     2461 tgaactatta ctgaaataaa aatcttcccg atgtttaccg taataaaaat tacggcgggt
     2521 ccctattatc taaaatcagg agtctgttat gcaaacgtgg ttaaatttgc aggataaaat
     2581 cattattgtc accggcggcg catccggtat tggtctggcg attgtagagg aattattagc
     2641 acaaggcgcg aatgtacaga tggtcgatat tcacggtggc gatggtcaat atgaaggtca
     2701 taaaggctat cagttttggc cgaccgatat ttctagcaca aaagaggtaa atcatacggt
     2761 agcggaaatt atccagcgtt ttggtcgcat cgacggtctg gtcaataacg ccggggtcaa
     2821 tttcccgcgt ctgctggtcg atgagaaagc gcctgccggg cagtatgaac tcaacgaagc
     2881 tgcattcgaa aaaatggtca atatcaacca gaaaggtgtt tttctgatgt cgcaggcggt
     2941 ggcgcgacag atggtcaaac aacatgatgg cgtgattgtg aatgtttcct cagaaagtgg
     3001 gctggaaggc tcagaaggac aaagctgtta cgccgctacc aaagccgcgc tcaatagctt
     3061 cacgcgctcc tggtcgaaag agctgggtaa acacggtatc cgtgtggtcg gtatcgcgcc
     3121 cgggattctg gaaaaaaccg ggctacgcac gccggaatat gaagaggcgc tggcgtggac
     3181 gcgcaatatc accgtcgagc agctgcgtga aggctatacc aaaaacgcca ttcctattgg
     3241 gcgcgccgga agattagcag aagtagctga ttttgtttgt tatctgttgt ctgaacgcgc
     3301 cagctacatc accggagtaa ccactaacat tgcgggcggc aaaacgcgcg gctaaggagg
     3361 ctttatggtc aacgccattt tttgtgccca cggcaaatta gcttgcgcca tgctggaatc
     3421 ggttcagatg gtttacggca acgccaatgt tgaagccgtt gcatttgtgc ccggtgaaaa
     3481 cgccggagac attgcggtaa agctggagaa gttagtaagc attcacaatc aagatgagtg
     3541 gctgattgcc gtggatttac agtgcggcag cccgtagaat gcggcagcaa tgcttgcgat
     3601 gcgcaatcct cgtttgcggg taatcagcgg cctttctctg ccgctggcgc tcgaactggt
     3661 tgataaccag gacagcatga atgttgatga actgtgtgaa cacctgacgc aaatcgcaaa
     3721 gcaaacctgc gttgtctgga aacaactggc aacgacagag gaggatttct gatgaacatt
     3781 acgctcgccc gcattgatga ccgtctgatc cacggccagg tcactaccgt ctggtcgaaa
     3841 gtggctaacg cccagcgcat tattatctgc aatgacgaag tttataacga tgaagtgcgc
     3901 cggacattat tgcgtcaggc tgcgccaccg ggaatgaagg ttaacgtggt caatattgaa
     3961 aaagccgtcg cggtttatca caacccgcaa tatcaggacg aaaccgtttt ttatcttttc
     4021 actcgcccac aggatgcctt agcgatggtt cgccaggggg taaaaatagg caccctgaat
     4081 attggcggca tggcctggcg gcccggtaaa aaacagttaa ccaaagcggt ttcattagat
     4141 gatgatgata ttaacgcatt tcatgagtta aataatcttg gtgtcatact ggatttacgc
     4201 gtcgtagcgt cagatccctc aatcaatatt atcgacaaaa taaacgaaca gttaatcgca
     4261 aattaaataa tagcgcctgt gtacatatgc tttaacaggc atggatgacg acacttaaaa
     4321 ggtgacaaat catggaaata agtaccctac aaatcattgc tatatttctt ttttcctgta
     4381 ttgccggaat gggcagcgtg ctggatgaat ttcagactca tcgcccgtta attgcctgta
     4441 cggtgattgg tttaattctc ggtgatttaa aaaccggaat aatactcggc ggtacgctgg
     4501 aattgatagc tctctgctgg atgaacgtcg gcgcggcgca atctccggat tctgcactcg
     4561 ccagcataat ctccgccatt ctggttatcg ttggtcagca aagcatcgcc accggaatcg
     4621 ccatcgcgtt gcctgtggct gcggcaggcc aggtgctgac ggtgtttgcc cgtaccatca
     4681 ccgtagtgtt ccagcacgcg gcggataaag cagcagaaga ggcgcggttt cgcactctcg
     4741 atattctgca tgtctccgcg cttggcgtgc aggcgctgcg cgtcgccatt ccggcactga
     4801 ttgtctcact gttcgtcagc gccgatatgg tcagcaatat gttaaacgcc attcccgaat
     4861 ttgtgacccg aggactacag attgctggcg gttttatcgt ggtggtcggt tacgccatgg
     4921 tgctacgtat gatgggcgtg aaatatttga tgcctttctt tttcctcggt ttcctcgcag
     4981 gtggctacct cgatctcagc ctgctggcgt tcggtggcgt cggcgtgatc atggccctgg
     5041 tctacatcca gctaaatcca cagtggcgta aagctgaacc tcaactccag gccaccgcct
     5101 ccaccgccct tgaccaactt gacgattaac ggagccatca tggaacagag aaaaattaca
     5161 cgcagcgatc tggtgagcat gtttctgcgc tccaacctgc aacaggcgtc ctttaacttt
     5221 gaacgtattc acgggctggg cttttgctac gacatgatcc ccgccatcaa gcgactttac
     5281 ccattaaaag aggatcaggt tgcggcgctc aggctacacc tagtgttctt caataccacg
     5341 ccagccgtat gtggcccggt catcggcgcg gaaattgatg acggtaccat caacggcatc
     5401 aaagtcggtc tgatgggacc attggcagga gttggcgatc cactggtctg gggaacgctg
     5461 cgcccgatta ccgccgcgct cggcgcatct ctggcacttt cgggcaacat tctcggcccg
     5521 ctgctgttct tctttatttt caacgcggtg cgtctggcga tgaagtggta tggcctacag
     5581 ctcggctttc gcaaaggggt gaatatcgtc agcgatatgg gcgggaatgt gctgcaaaaa
     5641 ctcaccgaag gcgcgtcgat tctcggactg tttgtgatgg gcgtgctggt gaccaaatgg
     5701 acgtcaatca acgtaccgtt ggtggtttca caaacgcatg ccgccgatgg ctccaccgtc
     5761 accatgaccg tgcagaacat tctcgaccaa ctttgccctg gtttgctggc gctcggtctg
     5821 acgctactaa tggttcgtct gctcaacaaa aaaattaacc cggtatggct gattttcgcc
     5881 ctgtttggct tagggattat cggcaatgct ctgggcttcc tgtcctgatt cttcgccccg
     5941 gcacgactgc cgaggccatc gctcaacatg aggtggttta tgaaaacaac agctctgcgt
     6001 ctttatggta aacgtgattt acgcctggaa acctttgacc ttcctgaaat gcaggaggat
     6061 gaaatcctcg caacggtggt cactgacagc ctgtgcctct cttcctggaa agaggccaat
     6121 ctgggtgaaa accataaaaa agtacccgac gatgtgacga ccaacccaat catcatcggc
     6181 cacgaatttt gcggcgatat tctggccgtg ggtaaaaagt ggcagcacaa attccagccg
     6241 ggtcagcgtt atgtgattca ggccaacctg caactccccg accgcccgga ttgccccggc
     6301 tactccttcc cgtgggtaga cggcgaagcg acgcatgtgg ttattcccaa cgaggtcatg
     6361 gaacaatatt gcctgctggc atacgacggc gaaacctatt ttgaaggctc actggttgaa
     6421 ccgctttcct gcgtgattgg cgcgttcaac gccaactatc atcttcagga aggtagttat
     6481 aaccacacga tggggattcg cccgcaaggg cgcacgctga tcctcggcgg caccggacca
     6541 atgggactgt tggcgattga ttatgcgctg catggaccag ttaacccgtc gctgctcgtc
     6601 attaccgata ccgacaacga caaattgagt tatgcgcgca agcactatcc atcagaaccg
     6661 caaacactga ttcattatct caatgccacc gatgcagcat ttgatacgtt aatggcgctg
     6721 agtggcggtc acggcttcga tgatattttc gtctttgtgc ctaatgaagg actggtgact
     6781 ctcgcctctt ccttgctggc gacagatggt tgcctgaatt tcttcgccgg accgcaggat
     6841 aaacatttca gcgcgccaat taatttctac gatgtgcatt atgcatttac ccactacgtg
     6901 ggcacgtcag gcggcaatac cgacgacatg cgcgcggcgg tcaaactgat cgaagagaaa
     6961 aaagtgcagg ccgcaaaagt ggtaacacat attcttgggc tgaatgccgc gggcgaaacc
     7021 acgcttgaat tgcctgccgt cggcggcggc aaaaagctgg tgtataccgg gaaatacctg
     7081 ccgctgacgt cactcacgca gattcaggat caagcactgg cggcgattct ggcgcgtcat
     7141 caggggatct ggtccggtga ggcggagcaa tacctgctcg cccatgcaga ggcaatttcc
     7201 catgattaat cgcgctactt aactctgcat gtcgctggct ggtcgcccca gcaacttcgc
     7261 gaccggatgt gcggctgatt gctgaagcag cacaatttgc acgagaagga taaaaaatat
     7321 cagcgcctta aggggcgctc aaattggtgg caaagtacac attgtttatg ccggatgcgg
     7381 aatgaagcct tattcggtct acataatttt gaaaatccaa tatattgcag ttatctggta
     7441 ggccaggtaa gcgtagcgca tcaggcaatg ctgtgtttgt catcagtctc agcgcccggt
     7501 gcaatacgtg acatcgtgag ccatgtcagt aagtggttaa attcgcagtg gtagcatgcc
     7561 ttccgtgatt tcttactaac cggagaagta atggaactgc ttttattgag taactcgacg
     7621 ctgccgggta aagcctggct ggaacatgca ctgccgctaa ttgctgaaca gttgcagggt
     7681 cgccgctcag cggtgtttat ccctttcgct ggcgtaacgc agacctggga tgattacaca
     7741 gagaaaacgg ctgcggttct cgctccgctg ggtgtttctg tcaccggtat tcatagcgtt
     7801 gtcgatcccg ttgccgcgat tgaaaatgcg gaaattgtga ttgtcggcgg cggtaatact
     7861 ttccagttgc tgaaacagtg ccgcgagcgc gggctgctgg ctccaattac tgacgtggtt
     7921 aaacgtggcg cgctgtatat tggctggagc gcaggcgcta accttgcttg cccaaccatt
     7981 cgtaccacca acgatatgcc gattgtcgat ccgcaaggtt tcgatgcgct aaatctgttc
     8041 ccgttgcaaa tcaacccgca cttcaccaac gcgctgccgg aaggccataa aggtgaaacc
     8101 cgtgagcagc gtattcgcga actgctggtc gtcgcgccag aactgacgat tattggtctg
     8161 ccggaaggta actggatcac agtgagtaaa ggtcacgcta cgctgggtgg cccgaacacc
     8221 acttatgtgt ttaaggctgg tgaagaagcg gttccgctgg aagctggtca ccgtttttaa
     8281 gtgaattgcc ggatgcatga tccggcatct tgttcatagt gcctgatgcg acgcttgacg
     8341 cgtcttatca ggcctacagg attcacaata ttaatactcg tcccgtcctt catcttcatc
     8401 cggctgttcc agcacactgt aagccaccga acaaaacagc gagttcaggc gctgcatatc
     8461 tcccagtaag cctaaatgta gcgaactggt ttcaatgctc tgtacgtttt gctgatgcag
     8521 gcgatcgacg tgggcgtggg aatagcggcg attaagaatg cgaaaacgat gtttgctgcg
     8581 acgcaaacga cgagcgctgg tgacatcgcc agagaagaac accgacattg ccagctttaa
     8641 attactgagc aattgctcat agagcgcatc cagttccttc aacccatcaa gcgaaaatgc
     8701 ccgccgtgct gccagcgatt tatcggcaat ttcgctgccc atgcgctcga cgatatcgga
     8761 ggcctgttca aggttgagcg acatttcgat gatctccgcc cagcggcgcg actcttcctc
     8821 cgccaactct tcttttggca tccgcgccag atacagctta atggcggtgt agagaacgtt
     8881 gatatcatct gccagcttac gcagctcttt ctcctgccgt ggctcgccgt gcatcacttt
     8941 attcagccct tccatcatct gttccatggc gtcgccaatg cgcagggttt cgcgcgcggc
     9001 gttcgccaga gcaagcgtgg gcgtgtccag cgcgctgaca tccagatgtt taggccgtag
     9061 ctgggtatcc agttccggtt catcgcgaat aatcgttttg caaaaccgtg ccatcgggtc
     9121 aacaaatggc agcatgacca ggcaacgtac aaggttgtag aagacgtgga aatagatcac
     9181 cagttccgct tttggcaatg acagcttccc cattgtctct gccagcaaat ggacaaacgg
     9241 caggataatc aggctaccca ccagcttaaa cagcagacta cccagcgcga cacggcgggc
     9301 tgcggcattg gcggcactgt tgttgagcat cgccagcagg ccggaaccga ggttagctcc
     9361 aatcaccaga cagagcgcca cggggaagga gataatgcct gcagcggtca gagtcgcggt
     9421 cagcagtaca gcagcaaggc tggagtagct gataatggcg aacatcgcgc caatcagcgc
     9481 atccagcaga atatcgccgg tcagcgaggc aaagatcacc tgaacgccgt ttgcctgggt
     9541 gatcggcgtt acggcctgca caatcaactc cagcgccagc aaaatcagcc caagaccaat
     9601 accgacgcgg cccagttgcc cggcgcgtga ctgtttgcgt ccgaggaaaa aaatcacgcc
     9661 gataaaaata agtaacggtg acagccagga taagtcgaag gtgagaatac gcgccattag
     9721 cgccgtcccg acatctgccc ccagcacaat gaccagagcc ggtgcgaggg ctaccagatc
     9781 ctgagcgacg aacgaggtca ccagcatggt ggtggcatta ctgctctgta ccagtgcggt
     9841 aacgccgatc cccgcgcaaa aggcgagcgg ctttttttcg acgctccggc taaggacagt
     9901 acgcaaacgc gcgccgaaga cgcgcattac gccggttcga acaatatgag tcccccagac
     9961 cagcagggcg acggcagaaa gcaggtgaag cagcgttaac acgagttcag gttctcctta
    10021 tcgttatatg ttcctgaagc aattgaatga ccaaaggacc ggaaacacgc tgactggcat
    10081 ctttactgtg ccttttgaca ttcgtttttc cggctaagat tattccagcc tgtttccagt
    10141 ataagtgttt catattaata aagagacagg gcactcatta agcgccctgt ttgttgtaag
    10201 gaaaagtgac agatttgtga atcagtccgc gtcatacccc agattcggtg ccagccagcg
    10261 ctcaacatcg gagacgctca tacctttacg gcgggcatag tcttcaacct gatcgcgctg
    10321 aatttgcgcc accgcgtagt acttgctgtc cggatgactg aaataccaac ccgaaaccga
    10381 tgcaccaggc cacatcgcga aagattctgt gagtttcatg ccagtatgtt tttccacttc
    10441 cagcagctcc cagatggtgg ctttttccgt atgttccggg caggccggat agcccggtgc
    10501 cggacggatg ccctggtagt tttcgcggat cagttcttcg ttgctgaggt tctcgttcgg
    10561 cgcatagccc cagtagactt tacgcacacg ctcatggaga tactccgcaa aggcttcggc
    10621 taaacggtcg gcaagcgctt tcaccatgat tttgttgtaa tcatcgtgct gcgcttcaaa
    10681 ggcatcagcc agtgcgtcct cttccagccc gccagtcacg gcaaatgcgc cgatgtaatc
    10741 tgctttacca gaaagcttcg gcgcaacgaa gtcagcgaga cagtagttag cgaatcccgt
    10801 tttttcggtc tgttgacgca gatggtggct gacgttgatc acatgggtgc gcgtttcgtc
    10861 acggtagatt tcaatgtcat cgcccacacg gtttgccggg aacaggccca ccacgccacg
    10921 cggattcagc gttttctcgg cgcttaattt atccagcatg tcgttggcgt ctttaaacag
    10981 ccgctgcgcc tcaacgccca ccacttcatc ttccagaatg cgcggatact tcccggccag
    11041 cgaccaggtc ataaagaacg gtgtccagtc gatgtaatta cgcagcgttt cgatgctggc
    11101 ttccacttcc tgcacgccga gacggtgcgc caccggcggc gtgtaagcct gccagtcaaa
    11161 tgcgaaatcg ttatcgcgcg ccgcttccag cgtgaccggt ggtgtgcgtg gtttcttgcg
    11221 cccgtgctga atacgcacgg tttcgtactc cttgcaggta cgagcgacaa aatcatcacg
    11281 ctgagtatcg gaaagcagcg ccgctaccac accaacggtg cgcgaggcat tctgcacata
    11341 caccgtaggg ccgctgtagt tctgctcgat tttcaccgcc gtgtgcgctt ttgaggtggt
    11401 cgcgccgcca atcagtaacg gaatggcgaa gccctgacgc tccatctctt tcgccacgtt
    11461 aaccatctcg tccagcgacg gcgtgataag ccccgaaagg ccaatcaaat cagcattcac
    11521 ttctttggcg atacggagaa ttttttccgc aggcaccata acgccgagat cgacaatttc
    11581 gtagttgtta cattgcagca ccacaccaac gatattttta ccgatgtcgt ggacatcgcc
    11641 tttcacggtg gcgatcacca tcttgccgtt ggttttaccc tgctctttgc tggcttcaat
    11701 aaacggttcg agatacgcca ctgcctgttt catgacgcgc gccgatttga ccacctgtgg
    11761 caggaacatt ttgccttcgc caaacagatc gccgaccacg ttcatgccgt ccatcaacgg
    11821 gccttcaatc acttcaatcg ggcgcgtagc ctgctggcgg gcttcttcag tatcctgctc
    11881 gataaactcg gttatgcctt tgaccagcga gtattccaga cgtttattca cgtcccacga
    11941 gcgccactcc gcttgctggg cgttagcagt gtcgtccgct ttactgccgc gatatttctc
    12001 ggcaagctcc agtaaacgct cagtgccatc gtcacgacga ttaagaatca catcttccac
    12061 cgcgtcgcgc agttcagcgg gtaggtcgtc gtaaatcgcc agttgcccgg cgttgacgat
    12121 ccccatatcc atgccattgc gaatagcgta gtagaggaac actgcgtgaa tggcttcgcg
    12181 caccggatcg ttgccacgga atgagaaaga aacgttagat acgccgccgg aaatcagcgc
    12241 gtgcggcagt tcgcgtttga tgtcttcaca cgcgccgata aagtcctgcg cgtagttgtt
    12301 gtgctcttca ataccggtgg cgaccgcgaa gatgttcggg tcgaagatga tatcttctgg
    12361 cgggaagcca acctcttcgg tgaggatttt gtacgcccga cggcaaatct cgattttccg
    12421 tgcgcgagta tcggcctgtc cctgttcgtc aaaggccatc accaccactg ccgcaccgta
    12481 gcgacgcaac aatttcgcgt gatggataaa ggcgtcgacg ccctctttca tcgagataga
    12541 gttaacaatg cctttgccct ggatacactt cagacctttt tcaatgacat cccattttga
    12601 ggagtcgatc ataatcggca cgcgagcgat atccggttca ccggcaatca gattgagaaa
    12661 acgcaccatc gccgcttcgg catcgagcat cccttcatcc atgttgatat cgataatctg
    12721 cgcgccgttt tccacctgct gacgcgccac atccagcgcc tcgctgtatt tctcttcttt
    12781 gatcaggcgc ttgaacttag cagagccggt gacgttggtg cgttcaccca cgttcacaaa
    12841 caggctatcg tcgccaatgt tcagcggctc caggccagac aaacggcagg ctacgggaat
    12901 ttccggcagt ttgcgcggcg ctaatccttc tactgcacga ctcatcgctg caatatgttg
    12961 cggcgtggtc ccgcagcaac caccgacgat attgagaaag cccgcttgcg cccattcacg
    13021 tatctgtttt gccatcgtgt cggcgtcgag atcgtattca ccaaaggcgt taggcagccc
    13081 ggcgttcggg tgggcggtga cgtagcattc cgcaatccgt gatagttcct gcacgtactg
    13141 gcgcagttca tcgggcccca gcgcacagtt caggccaaag gtcagcgctt cggcgtggcg
    13201 caatgagttg taaaatgctt cggtggtctg cccggagagc gtgcgcccgg aggcgtcggt
    13261 gatggtgccg gagatcataa tcggcagctc aacgcccagc gcttcaaact ccgttttcac
    13321 cgcaaagacc gccgctttgg cgttaagggt gtcgaaaacg gtttcaatca ggatcagatc
    13381 cgcaccacct tccaccagcg ctttggtgga ttcgcgatag gccgccgcca gctggtcaaa
    13441 agtgatgttg cggaatgccg gatcgttgac gtccggagaa atagacgccg tgcggttggt
    13501 tgggccaaga acaccggcaa cgtagcgcgg tttctccggc gtgcgcgcgg tccactcgtc
    13561 agcacaggcg cgcgccagtt tcgccgccgc aaagttgatt tccgccgaca gggattccat
    13621 ctggtaatcc gccatcgcaa tggtcgtgga gttgaaggtg ttggtttcga tgatatccgc
    13681 gcccgcttca aagtaggcgt tgtggatagc ggcgatcact tccggtttac tgagtaccag
    13741 caggtcgttg ttgcctttga ggtcgcatgg ccagtcggca aagcgttcac cacgaaaatc
    13801 ggcttcgttc agtcgatagc tctggatcat ggtgcccata ccgccgtcca acaccagaat
    13861 acgttcattt aactgcgcac gcagttgttc cactttgctg ctcacacttg ctcccgacac
    13921 gctcaaccca gatttaataa aaattcaaca aaccatactg gaataaacgc atctgtggta
    13981 aaagcgacca ccacgcaaca tgagatttgt tcaacattaa ctcatcggat cagttcagca
    14041 actattgcat tagctaacaa taaaaatgaa aataatttcc acgatacaga aaaaggaggc
    14101 tgtcatggtc gcacccattc ccgcgaaacg cggcagaaaa cccgccgttg ccaccgcacc
    14161 agcgactgga caggttcagt ctttaacgcg tggcctgaaa ttactggagt ggattgccga
    14221 atccaatggc agcgtggcac tcacagagct gtcgcaacaa gccgggttac ccaattccac
    14281 gacccaccgc ctgctaacca cgatgcaaca gcagggtttc gtgcgtcagg tcggcgaact
    14341 gggacactgg gcaatcggcg cacatgcctt tatggtcggc agcagctttc tccagagccg
    14401 taatttgtta gcaattgttc accctatcct gcgcaatcta atggaagagt ctggcgaaac
    14461 ggtcaatatg gcggtgcttg atcaaagcga tcacgaagcg attattatcg accaggtaca
    14521 gtgtacccac ctgatgcgaa tgtccgcgcc tatcggcggt aaattgccga tgcacgcttc
    14581 aggtgcgggt aaagcctttt tagcccaact gagcgaagaa caggtgacga agctgctgca
    14641 ccgcaaaggg ttacatgcct atacccacgc aactctggtg tctcctgtgc atttaaaaga
    14701 agatctcgcc caaacgcgca aacggggtta ttcatttgac gatgaggaac atgcgctggg
    14761 gctacgttgc cttgcagcgt gtattttcga tgagcaccgc gaaccgtttg ccgcaatttc
    14821 tatttccgga ccgatttcac gtattaccga tgatcgcgtg accgagtttg gcgcgatggt
    14881 gattaaagcg gcgaaggaag tgacgctggc gtacggtgga atgcgctgac tttttctggc
    14941 gggcagaggc aatattctgc ccatcatacc tgagtggcaa tagaataagg gtgtctgtta
    15001 atcgcattga cgccaaaata acttaatgtc atacacttca cacactttta ttaactcagc
    15061 attattttta aacatcaaac cacttaatta ttaacaaaa
//
LOCUS       DAEEUE010000091        15050 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.091, whole genome shotgun sequence.
ACCESSION   DAEEUE010000091 DAEEUE010000000
VERSION     DAEEUE010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15050
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.091"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..176)
                     /locus_tag="KLK84_003093"
     CDS             complement(<1..176)
                     /locus_tag="KLK84_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010332.1"
                     /translation="MVANINLIKQESYSVVNLEKQLMSNKSPKQTLLNQFSLCLKMEG
                     EEQLCDCVNELKHG"
     gene            complement(592..1533)
                     /gene="fabY"
                     /locus_tag="KLK84_003094"
                     /gene_synonym="yiiD"
     CDS             complement(592..1533)
                     /gene="fabY"
                     /locus_tag="KLK84_003094"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HBE2010333.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1578..2015)
                     /gene="dtd"
                     /locus_tag="KLK84_003095"
     CDS             complement(1578..2015)
                     /gene="dtd"
                     /locus_tag="KLK84_003095"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBE2010334.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(2012..2884)
                     /locus_tag="KLK84_003096"
     CDS             complement(2012..2884)
                     /locus_tag="KLK84_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HBE2010335.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2878..3477)
                     /gene="yihX"
                     /locus_tag="KLK84_003097"
     CDS             complement(2878..3477)
                     /gene="yihX"
                     /locus_tag="KLK84_003097"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HBE2010336.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3576..4361)
                     /locus_tag="KLK84_003098"
     CDS             complement(3576..4361)
                     /locus_tag="KLK84_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBE2010337.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4395..5291)
                     /gene="yihV"
                     /locus_tag="KLK84_003099"
     CDS             complement(4395..5291)
                     /gene="yihV"
                     /locus_tag="KLK84_003099"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HBE2010338.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGLPDCDQTRSFLSLFV"
     gene            5459..6355
                     /gene="yihU"
                     /locus_tag="KLK84_003100"
     CDS             5459..6355
                     /gene="yihU"
                     /locus_tag="KLK84_003100"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HBE2010339.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGHGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6379..7257
                     /gene="yihT"
                     /locus_tag="KLK84_003101"
     CDS             6379..7257
                     /gene="yihT"
                     /locus_tag="KLK84_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HBE2010340.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            7274..8515
                     /gene="yihS"
                     /locus_tag="KLK84_003102"
     CDS             7274..8515
                     /gene="yihS"
                     /locus_tag="KLK84_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HBE2010341.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8653..9555
                     /locus_tag="KLK84_003103"
                     /pseudo
     CDS             8653..9555
                     /locus_tag="KLK84_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
     gene            9754..11790
                     /gene="yihQ"
                     /locus_tag="KLK84_003104"
     CDS             9754..11790
                     /gene="yihQ"
                     /locus_tag="KLK84_003104"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HBE2010342.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11836..13210
                     /locus_tag="KLK84_003105"
                     /pseudo
     CDS             11836..13210
                     /locus_tag="KLK84_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13253..14656
                     /locus_tag="KLK84_003106"
     CDS             13253..14656
                     /locus_tag="KLK84_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE2010343.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14724..>15050
                     /gene="ompL"
                     /locus_tag="KLK84_003107"
     CDS             14724..>15050
                     /gene="ompL"
                     /locus_tag="KLK84_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HBE2010344.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT         3699 a         3874 c         3744 g         3733 t
ORIGIN      
        1 aaaccatgct tcagttcgtt aacacaatcg cacagttgct cctcaccttc cattttaagg
       61 caaagtgaaa attgatttaa tagcgtttgc ttcggtgatt tattactcat tagttgtttt
      121 tctaaattaa caacagaata actttcttgt ttaatgagat taatattggc aaccattata
      181 gcctccttcc cacggtttaa gagcaaacat acaacataat gattcataac atacagagca
      241 gtaaatgaat gatactgaca atgtatgcca gcccattatt tttgcataat atttgtgagc
      301 tatagatttt aaaaatattt atgaaaaaag tatggcacca gagggggttt tgactatcaa
      361 aaatcgctca aacctgaact aatatgatga gttaagcatt atctgcaata attgccactc
      421 agctctacaa ataatattta aatgctcctg aacagtgatg tagcaagaca cgtatggtgc
      481 caacgaagaa taaaaattta taaagttctt attcaatata accggaatag tcagggactg
      541 atgtaaatga ccggaatgaa tgagagaggc agcaccgccc ctctccataa gctactcttc
      601 ttcgttcccg ccctcttcat acgggccaaa tggcttcgcg ggcagaacga tatacgtgcc
      661 ttcaaacact gcacccggcg tctcgtcgcc aaagatttcg acctgcatct gcacccgtgc
      721 ttttcgtccg cgcgccagac ggtcgagatc gccgcttaag gcaccaaggt cggctaccgc
      781 atggggttta ccgctaatcg gtttgctgta gcggatatgc gcatccgcca gaataatcgt
      841 tccgccgagg tggcgttcac gcagcatcag ccagataagc ccccaaccgg tgagcgtcgc
      901 cagcgagaat aaactcccgg caaacagcgt atggtgcgga ttctgattgc cggtttccgg
      961 catggtggtg ataaattttt gcccggtata ttgttgaata cgcacgccca ttttttcact
     1021 aagcgggata tgttcgtacc acgcctgttg cagctgcgcg caccagtcgc cgcgatgcaa
     1081 aatatcatcc agagtggcga cgggcttaat catcaaaaaa tggcgaatcg gcgtggtggt
     1141 tggcgtggtg atttctccct gattaacaaa ccccagcttg gcgaaaaact ccaccgcgtc
     1201 ttcacgggcg ctacaggtca cgcgcttaac gccttcctga cgcgccaccg actccagggt
     1261 catcgccatc agcgttccta accctttgtc ctgcacgtcc gggtgaacga ccataaagcg
     1321 aatggacgct tcattgtcgg cattaatata caatcggcct accgccacca gattaccctg
     1381 ctcgtcgacg accatctggt gatgcgccat cgcatcccac gcgtcgcgtt ccgaaccttt
     1441 tggttgatgc aggggcttac gcaacatttc ccagcgaaac tgatagtaac gctctaactc
     1501 ttcttctgtt tgtggaacct gaaggtgata catagcgata ctctctcttg tttcccgtga
     1561 caaccctgga agctggctca tacctgcaac cagaatgtca cggggccatc attgaccagc
     1621 gatacctgca tatcggcagc gaagcgtcct gtttgcgtgt tcatctcttg ctggcggcag
     1681 cgttcgacga aatagtcata taacgcctct gcgcgatccg gtgatgcgcc tttggagaaa
     1741 cttgggcgca tcccccgttc ggtatctgcg gcgagggtaa actgggaaac caccagtaca
     1801 ctgccgcccg cctgttgcac gttgagattc atcttgcctt cggcatcgct aaagatgcgg
     1861 tagccgagca cacgctcgca cagacggttt gctttctgtt cgtcgtcatc cttttcgaca
     1921 cccaataaca ccaaaagtcc cgcgccaatt tcgcccgtca cttctccctc cacggtgacg
     1981 ctggcacggg ttacgcgttg aattaatgca atcatggttc gtcgtcttct tcttgttcag
     2041 ctgcttgttt tagtttgcgg tattccccga gagtgacagt aatttccgcg ccaagcaaga
     2101 cgatacacca tgtccagtag acccaaacaa agagaatggg gatcaccgcc agcacaccgt
     2161 aaatgagctg atatgacggg aacatggtga tataaagcgc gaaacctttc tttcctgctt
     2221 cgaacaggag tgcggcgaca aacgcgccga caatcgcgtc gcggttaggt acgcggatgg
     2281 taggaacaat gctgtacagc aaccagaagg agatccacga caacagcaac ggaaaaatac
     2341 gcagcacgtt gtcgatgaca gtattgagat cgctcgccca gcgcagggag agcaaatagg
     2401 aactgatcgc cagactggcc cctgccagca gcggccccag cgttaaaatc atccagtaca
     2461 cggcgaacga gtaaattttt ggtcgcgctc gtttactgcg ccagatggta ttcaacgcgc
     2521 tatcgatgga gtacatcaac aataacgcgg tgacgatcag cccgcacgcc ccaacggcgg
     2581 tcatcttgtt ggaattagca acaaattgtt cgatataccg ctgaataaca tcgccagtag
     2641 caggcagaaa gttggcaaaa ataaagtgac gtaactgaat gctgacgtcg gaaaacatgg
     2701 gaaaagcggc gaaaagcgca aaaacaacgg caaccagcgg cactaatgag agcaacgaca
     2761 cataggcaag gttacctgcc agggttgtca tgttgtcctc atcaatacgt tgccagagta
     2821 gttttagcca ggcccatagt ggacgggtac ggtgcctggc tttgtcctga atggttttta
     2881 gcataacacc ttcgcgaaat agtccgggat ggtggtttta tctttcacca gaatactggt
     2941 aatgcctagc tgattagctc cttctatatt atcggcgtta tcgtcgaaaa agaccgtatc
     3001 gctgggtgaa aaaccttccg cctgcaaaac atgctggtaa attcgtgctt caggtttgcg
     3061 catccccaga tcttgcgaca gatagatatg gtcagcagca tcacgaattt ccgggtattc
     3121 ttccggccag aaggtggtat gcaggcggtt ggtattggaa agcaccacca cgcgatgccc
     3181 ctgctcacgc agtttatgca tgatggcgat cacttccggg cgcagcgcaa caaacaccgc
     3241 ctgccagccg tgagagaact gctcgtagct tagcggtaga gccatctcat gacacagcgc
     3301 ctctgcgaac gcttcatcgc taatttcccc acgctcatgc tgatgaaacg cctcccccat
     3361 atgaaaactc ttcttaagcg atgccagcgg aatacgcgtt aaatcgctcc aggctcccag
     3421 cacacggtta aagtcgatat cgacaatcac attacctaaa tcaaagatat agagcatttt
     3481 tgcctccttt cgcgccacaa gaaattaact gtagcgggaa aggatgggtt tggctatgtg
     3541 gcaaggtatg aagccaggtt aacggtcaca aaaatctacg cgtcttcctg gggaagaatg
     3601 atttcaattt ggctgtcttg taagcgtgat tttagcgcag cgggcggcag ctcgtcggta
     3661 aagagcgcag tgacctgtgc cacgttacca atttcaaccg ccgccgaagc atgatactta
     3721 gtgtgatcgg cgaccagcag aatatttctc gcgtgcgcca tcatcgtttt caccacgtta
     3781 gcttcgttta catcaaactc catcaacgcg ccatcgctct caatcgcccc aacgcttgtt
     3841 accagataat cagcacgaaa atcagccaca aaggacgccg ctgaagggcc aatgatcccg
     3901 ctattatgag agcgcaacgt accgccgggc accatcactt caaagcgcgg gttgtggtaa
     3961 agaatatgcg ccacacgcag gctgttggtg attatccgca aatgattatg gttaagtaac
     4021 gcccgggcaa catgctcaac agtcgtacca atggtgataa atattgttga accatcagga
     4081 atatagtctg ccaccgcttc ggcaatcgct ttttttttct cggtttgcga aacctcacgc
     4141 tgctcgaacg ccgtattaac gacgctggaa gcccgacccg cgccaccgtg atggcgcgta
     4201 atcaggcctt gctcgcttaa tttacgaata tcccggcgga ccgtctgcgt ggagacatcc
     4261 agcagatttg ccagctcatc aatattcata tacccacgct cggcgatcag catgaggagt
     4321 tggtcgtgcc gcgggttacc ggttagttcg gtaaggctca tgaaaattcc tcgaaaaacc
     4381 atcaccctgg cattttatac aaaaagtgac aaaaaagatc gggtttgatc acagtcaggg
     4441 agcccggctc gtccaccggg acgtgtgcat tttaacgccg ctacaccgct ggcgaagcgg
     4501 actgactccg ctaaatcccc acttgttgcc agcgccaccg ccaaagcgcc gtgaaaaaca
     4561 tcacccgcac ctgtggtatc taccacatca actttgaagg ccggttgatg ctgacgccca
     4621 ccattttcca gccagtcgca gcctgcgcta ccctgggtca catagacatg accatttgtg
     4681 agcgtttgtg cctgttttag cgcactggcc atctctttca cgcccgttaa gcgcgccaga
     4741 cccggttctg aaaaggccgc gtgatcgctt aatgccacca gctcactgat atcctgcggc
     4801 gtaatgtccc cgtccagaac ggtcatcaca cccgcctgac gggccagggt gaaggctttt
     4861 ttagcgccgt cgtgccagcg tacatctgcc agcacaacat cccactgaga gaaatcaatt
     4921 tcctccaacc actctgcgtc aggcagcaga tccgggctgg ggtagttaat gattatccgc
     4981 tcgcctttgg tatccaccat gatggcggat tgcgaagatt tcgcctggtt ataccgtttg
     5041 gtgtaacggg tgttaacgcc ccaggattcc agctctgcca gcaggctgtt gcccgcgtcg
     5101 tcatcaccta cgcgaccaat aaaatcgacc tgcgccccca gccttgccgc cgcaaccgct
     5161 gccgtcgccg ctggcccgcc accaacttcc gtataatttc tcgccacgta tttaccgctc
     5221 tccgtcggta acccttccac gtaatagatg cgatccatca cggttatacc tacacaagca
     5281 acacgaatca tggtcattcc ttaaacattt tgggatgcgt tcattttaat ttcaccaatt
     5341 gtttaaaaag tgacggctgt caattttttg acgataaatt acaaattcga tcaaaaacag
     5401 acaagcaaag catgtcataa atgacaaaaa gtgacatcac tgtattcagg agagggttat
     5461 ggcagcaatc gcgtttatcg gtttaggaca aatgggttcg ccaatggcga gcaatttatt
     5521 gcagcaaggg catcaacttc gcgtctttga tgtgaacgcc gaagctgtgc ggcatctggt
     5581 agacaaaggt gcgactcccg ccgccaaccc ggcgcaggcc gctaaagatg ccgaatttat
     5641 cattaccatg ttgccgaatg gcgatctggt gcgcaacgtg ttgttcggtg aaaacggcgt
     5701 ttgcgaaggc ttatctaccg atgcgctggt cattgatatg tccaccatcc atccgctgca
     5761 aaccgataaa ttgattgccg atatgcaagc caaaggcttc agcatgatgg atgttccggt
     5821 aggccgtact tctgcaaatg ccattaccgg tactctgtta ctgctggctg gcggcaccgc
     5881 tgaacaagtt gaacgtgcca caccgatcct gatggcgatg ggcagtgagt tgatcaacgc
     5941 tggcggtccg ggcatgggga tccgcgttaa gctcatcaac aactacatga gcatcgcgct
     6001 caatgcgctt tcggcagaag ccgccgtttt gtgcgaagcc ctgaatcttc ccttcgatgt
     6061 tgccgtcaaa gtgatgagcg gtaccgccgc cggtaaaggt cacttcacca cttcctggcc
     6121 aaacaaagtc ctcagcggag atctttctcc cgccttcatg atcgatcttg cccataagga
     6181 tcttggcatc gcccttgatg tcgccaacca gctgcatgtg ccaatgccgc tgggggccgc
     6241 ctcacgggag gtttatagcc aggcgcgcgc agcgggtcac ggtcgccagg actggtccgc
     6301 cattctggaa caggtccgtg tcagtgccgg gatgactgcc aaagtaaaaa tgtaacgact
     6361 ggataaagga ataaatgaat gaataagtac accatcaacg acattacgcg cgcatcgggc
     6421 ggttttgcca tgctggcggt cgatcagcgc gaagccatgc gcatgatgtt tgccgcggct
     6481 ggggcacccg ctccggtagc cgatagcgtt ttaactgatt tcaaagttaa cgctgcaaag
     6541 gccctctcgc cttatgcctc ggcgattctg gtagatcaac aattctgcta ccgccaggtg
     6601 gttgagcaaa acgcgattgc caaaagttgc gccatgattg tcgccgccga tgagttcatt
     6661 cctggcaacg gtattccggt cgatagcgtg gttattgacc gcaaaatcaa tccgctacag
     6721 atcaaacaag acggtggcaa agccttaaaa ctgctggtgc tgtggcgtag tgatgaagat
     6781 gcgcagcaac gtctggatat ggtgaaagag ttcaacgaac tgtgccactc acacggtctg
     6841 gtaagcatca ttgagccagt cgtccgccca ccgcgtcgtg gcgataaatt cgatcgcgaa
     6901 caagcgatca tcgatgccgc caaagagctg ggcgacagtg gcgctgacct ctacaaagtt
     6961 gaaatgccgc tttatggcaa aggtccgcaa caagagcttc tctgtgcttc acaacgtctg
     7021 aatgaccata tcaatatgcc atgggtgatc ctctcttcca gtgtcgacga aaaactgttc
     7081 ccgcgtgccg tacgcgtggc aatgacggcg ggcgcatcgg gattcctggc aggccgtgca
     7141 gtctgggcat cggtcgtcgg tttaccagac aacgagctga tgctccgtga cgtttgcgca
     7201 ccgaaattac aacaacttgg cgatatcgtc gacgaaatga tggctaaacg ccgctaagat
     7261 gaggataaaa ggaatgaaat ggtttaacac cctaagccac aaccgttggc tggaacagga
     7321 aaccgaccgc atctttgatt ttggtaaaaa ttccgtagtg ccgactggtt ttggctggtt
     7381 aggcaataaa gggcaaatca aagaagagat gggcacccat ctgtggatca cagctcgtat
     7441 gttgcacgtt tattccgttg ctgcggcgat gggtcgacct ggcgcttacg cgttggttga
     7501 tcacggtatt aaagccatga acggcgcact gcgtgataaa aaatatggcg gctggtatgc
     7561 ctgcgtgaat gacgagggcg tggtggatgc ctccaaacag ggctatcaac acttctttgc
     7621 tctgctgggt gccgccagcg ccgtcacaac gggtcacccg gaagcgcgca agctgctcga
     7681 ttacaccatt gagattatcg agaaatattt ctggagcgaa gaagagcaga tgtgcctgga
     7741 gtcctgggac gaagccttca gcaaaaccga agagtaccgc ggcggcaatg ccaatatgca
     7801 cgcggtggaa gctttcttga ttgtttatga cgtcactcac gacaaaaaat ggctggatcg
     7861 cgcgattcgc gtggcttccg tgattatcca cgacgtcgcc agaaataatc attatcgcgt
     7921 taacgaacac ttcgataccc agtggaatcc gctgccggat tacaacaaag ataacccggc
     7981 gcatcgcttc cgcgcgttcg gaggtacacc aggccactgg atcgaatggg gccgtttaat
     8041 gctgcacatc catgctgccc tggaagcccg ttgcgaacaa ccaccagcat ggctgctaga
     8101 agatgccaaa ggtctgttta acgccaccgt gcgcgatgcc tgggcacccg atggagcgga
     8161 cgggattgtt tataccgttg actgggaagg aaaaccggtg gtccgcgaac gtgtacgttg
     8221 gcctatcgtc gaagcaatgg gtacggccta cgcgctctac accgtcactg gcgatcgcca
     8281 gtatgaaacc tggtatcaaa catggtggga gtactgcatt aagtacctga tggactatga
     8341 aaatggttcc tggtggcagg agctggatgc ggacaataag gtcatcacca aagtctggga
     8401 cggcaaacag gatatttatc acctgctgca ttgcctggtg atcccgcgta tcccgttagc
     8461 gcccggcatg gctccagcgg ttgcagcggg tctgctggat attaatgcga aataactgat
     8521 ctgaaaatag ggtgcgggtt ttcccctcac cctaaccctc tccccaaagg ggcgagggga
     8581 ccgtccggtg cagtttttgg gtttgacatc agcctcacgg gaggctgtat gtcgttaatt
     8641 aaggtgcctt gtatgcaaat caccaatatg cattgcagtg gacagaccgt ttcacttgcc
     8701 gcgggggatt atcacgcgac cattgtgacc gtcggtgctg gtctggctga attaaccttt
     8761 cagggttgtc atttagtcat cccgcacaaa cccgaagaga tgccgctggc gcatctgggt
     8821 aaagtgctta ttccgtggcc gaaccggatc gccaacggct gttaccgtta tcaggggcag
     8881 gaatatcagt tacccattaa tgaacatagt tcaaaagccg ctattcacgg gctgcttgcc
     8941 tggcgagact ggcaaattag tgaattaacc accaccagcg ttaccctgac agcttttctg
     9001 ccgcccagtt acggctatcc gtttatgctg gcttcacaag ttgtttattc gctcaacgca
     9061 cacacgggtc tttccgttga gatcgcaagc cagaatatcg gcactgtcgc agcgccttat
     9121 ggcgtgggta ttcatcctta tttaacctgc aacttaacat ctgttgatga atatctgttc
     9181 cagcttccgg caaactaggt ttatgccgtc gatgaacacg taaatccgac aacgcttcac
     9241 cacgttgatg agctggattt aaatttcacg caggcgaaaa aaatagccgc gacaaaaatc
     9301 gaccacacct ttaaaaccgc taatgatttg tgggaaatga ccattaccca tccacagcag
     9361 gcgctttcgg taagcctttg ttccgatcaa ccctgggtgc aggtttatag cggtgaaaaa
     9421 ttacagcgtc agggactggc agttgaacct atgagttgcc cgcctaacgc ctttaattca
     9481 ggtatcgact tattattact ggaaccagga aaaccgcacc gcctgttttt taatatttac
     9541 ggacagagaa aataacttgt attgattagc agtcaaaatc attgaagaac atttcactcg
     9601 ctaaaggcaa cattatgtat cttcaattta ttaagttaaa aacagctcag cttctgtaaa
     9661 gtttttatat atcagtttta tgagaatcat tttacataag cctgtaataa caggaaggca
     9721 acaatctcgt tctcaactct atggagatta gttatggata cgccacgtcc acagttatta
     9781 gattttcaat ttcatcagaa taacgacagt tttaccctac attttcaaca acgtcttatt
     9841 ttaacccata acaaagataa tccttgttta tggattggct caggtatagc ggatatcgat
     9901 atgttccgcg gtaatttcag cattaaagat aaactacagg agaaaattgc gcttaccgac
     9961 gccatcgtca gccagtcacc ggatggttgg ttaattcatt tcagccgtgg ttctgacatt
    10021 agcgccacgc tgaatatctc tgccgacgat cagggccgtt tattgctgga actacaaaac
    10081 gacaacctta accacaaccg tatctggctg cgccttgccg ctcaaccaga ggaccatatc
    10141 tacggctgcg gcgaacagtt ttcctacttc gatctgcgcg gcaaaccgtt cccgctatgg
    10201 accagtgaac aaggcgttgg tcgcaacaaa caaacctatg tcacctggca ggccgactgc
    10261 aaagaaaatg cgggcggcga ctattactgg actttcttcc cacagcctac gtttgtcagc
    10321 acgcagaagt attactgcca tgttgataac agttgctaca tgaacttcga ctttagcgcc
    10381 ccggaatacc atgaactggc gctgtgggaa gacaaagcta cgctacgttt tgaatgtgct
    10441 gacacataca tctccctgct ggaaaaatta actgccctgc tgggacgcca gccagaactg
    10501 cccgactgga tttatgacgg ggtaacgctc ggcattcagg gcgggacgga agtgtgccag
    10561 aagaaactgg acaccatgcg taacgcgggc gtgaaggtta acggcatctg ggcgcaggac
    10621 tggtctggga tccgtataac ctcttttggc aagcgcgtga tgtggaactg gaagtggaac
    10681 agcgaaaact acccgcaact ggattcacgc attaagcagt ggtatcagga gggcgtgcag
    10741 ttcctggcct atatcaaccc gtatgttgcc agcgataaag atctctgcga agaagcggca
    10801 caacacggct atctagcaaa agatgcctct ggcggtgact atctggtgga gtttggcgag
    10861 ttttacggcg gcgttgtcga tctcactaat ccagaagcct acgcctggtt caaggaagtg
    10921 atcaaaaaga acatgattga actcggctgc ggcggctgga tggctgactt cggcgagtat
    10981 ctgcccaccg acacgtacct gcataacggc gtcagtgccg aaattatgca taacgcctgg
    11041 cctgcgctgt gggcgaagtg taactacgaa gcccttgaag aaacgggcaa gctcggcgag
    11101 atcctcttct ttatgcgcgc cggttctacc ggtagccaga aatactccac catgatgtgg
    11161 gcgggcgacc agaacgtgga ctggagtctc gacgatggcc tggcgtccgt agtcccggcg
    11221 gcgctgtcgc tggcaatgac cggacatggt ctgcatcaca gcgacattgg cggttacacc
    11281 accctgtttg agatgaagcg cagcaaagag ctgctgctgc gctggtgcga tttcagcgcc
    11341 ttcacgccga tgatgcgcac ccacgaaggt aaccgtcctg gcgacaactg gcagtttgac
    11401 ggcgatgcgg aaactatcgc ccattttgcc cgtatgacta ccgttttcac caccctgaaa
    11461 ccttatctga aagaggccgt cgcgctgaat gcgaagtccg gtctgccggt catgcgcccg
    11521 ctgttcctgc attacgaaga cgatgcacat acctattccc tgaaatatca gtacctgtta
    11581 ggccgcgaca ttctggtcgc tccggtacac gaagaaggtc gcagcgactg gacgctctat
    11641 ctgccggagg ataactgggt acatgcctgg acgggtgaag cgttccgggg cggggaagtt
    11701 accgttaatg cgcccatcgg caagccgccg gtcttttatc gcgccgatag cgaatgggcg
    11761 gcactgttcg cgtcgttaaa cagcatctaa tcacatccgc ccgtggggaa actcatgggc
    11821 aaagggagaa catcgatgag tcacatcaca acggaagatc cagcaacttt acgcctgccc
    11881 tttaaagaga aactctctta cggtattggc gacctggcct ctaacatcct gctggatatc
    11941 ggtacgcttt atcttttgaa gttttatacc gacgttctgg ggctgccagg cacctatggc
    12001 ggcattatct ttttgatttc aaaattcttt actgcgttta ccgatatggg taccggcatt
    12061 atgttggatt cccgacgcaa gatcggtcca aaaggtaagt tccgtccttt tattctgtat
    12121 gcgtcattcc cggtcacctt actggcgatc gccaactttg tcggcacacc gtttgatgtc
    12181 accggtaaaa cggtgatggc cactattctg tttatgcttt acggactgtt tttcagcatg
    12241 atgaactgct cctacggcgc aatggtcccg gcaatcacca aaaaccccaa cgaacgcgcc
    12301 tcactggcgg catggcgtca gggcggcgct acgctgggcc tgctgctgtg cacggtggga
    12361 ttcgtgccag ttatgaatct tatcgaaggt aatcagaaac ttggctatat cttcgccgcc
    12421 acgctgtttt cactgttcgg cctgctgttt atgtggatct gctactcagg cgtgaaagag
    12481 cgttatgtcg aaacccaacc aaccaatccg gcgcaaaagc ctggcctgtt gcaatctttc
    12541 cgcgcaattg ccggtaaccg cccactgttc attctgtgta ttgccaacct ctgcacctta
    12601 ggggcgttta acgtcaagct cgccattcag gtctattaca cccagtacgt acttaacgat
    12661 cccatcctgt tgtcgtatat gggatttttc agcatgggct gtattttcat cggcgtgttc
    12721 ctgatgcctg gcgcagtcag acgttttggt aagaagaagg tctatatcgg cggcctgctg
    12781 atttgggtgc tgggcgatct gctcaactat ttcttcggcg gcggctcggt cagctttgtg
    12841 gcgttctcct gcctggcgtt cttcggctca gcgcctgaac tgggcgctgg tttccgacac
    12901 cgtcgagtac ggcgagtggc gcaccggcgt gcgttcggaa ggcacggtct acaccggctt
    12961 caccttcttt cgcaaagtgt ctcaggcgct ggctggtttc ttccccggct ggatgctgac
    13021 gcaaattggc tatgtgccga acgtcgcaca ggctgaccac accattgaag ggttacgcca
    13081 gttgatcttc atctacccaa gcgcactggc ggtagtcacc attgtggcga tgggttgctt
    13141 ctacagcctg aacgagaaga tgtatgtccg cattgtggaa gagatagaag cccgtaaacg
    13201 cacggcgtaa taataataaa cgacgccctg cggggcgtta taaggagtga ttatgtctga
    13261 ccataatcca ctgacattaa aactgaatct gcgggaaaaa atcgcctatg gtatgggcga
    13321 cgtcggttcg aatttaatgc tctgcatcgg tactctgtat ctcctcaaac tttataccga
    13381 tgaattaggg atgcctgctt actatggcgg gattatcttt ctggtcgcga agttttttac
    13441 cgcgtttacc gatatgctca ccggattttt actcgactcg cggaaaaata ttgggcctaa
    13501 aggcaaattt cgtcccttta ttttatatgc agcagttccg gcggcgttaa ttgcaacgct
    13561 ccagtttatc gccaccacct tttgtttgcc ggttaaaacg acgattgcca ccacgctgtt
    13621 tatgatgttt ggactttcat acagtctgat gaactgctcg tatggtgcga tgatcccggc
    13681 aattaccaaa aacccgaatg agcgcgcgca gctcgcggct taccgccagg gtggtgcaac
    13741 catagggcta ttgatttgta ccgtggcgtt tattccgttg cagtcgcttt tttctgactc
    13801 aaccgtcggt tatgcctgtg cggcacttat gttctccatt ggcggcttta tttttatgat
    13861 gctgtgctac agaggcgtca aagagcatta tgtggacaca gcgccaaccg gacataaagc
    13921 cagtattctc aaatcttttt gcgcgatatt tcgtaatcca ccattgctgg ttttatgcat
    13981 tgctaacctg tgtactctgg cggcatttaa tatcaaactg gcgattcagg tctattacac
    14041 ccagtatgtg ctgaatgata ttaatttatt gtcgtggatg ggattcttca gtatgggatg
    14101 catcctcgtc ggcgtattac tggtgccagt aactgtaaaa tgttttggta aaaaacaggt
    14161 ttatttagct ggcatggtgc tatgggcggt gggtgatata ctgaattatt tctggggaag
    14221 taactctttc actttcgtca tgttctcttg tgtcgccttt tttggcacgg cctttgtaaa
    14281 cagcctgaac tgggcactgg taccagatac cgtagattac ggtgaatgga aaaccggtat
    14341 tcgtgccgaa ggttctgttt ataccggtta taccttcttt cgtaaaattt ctgccgcact
    14401 tgctggcttc ttgccaggca ttatgctgac gcaaattggt tatgttccca acatcgcgca
    14461 aagcgatgcg acattacagg gtttgcgtca gctcattttt atctggcctt gtgcactggc
    14521 aattattgct gcattgacga tgggattctt ttacacactc aatgaaaaac ggtttgcatt
    14581 gattattgag gaaatcaacc aacgcaaaaa taaagagatg gcgacagaaa aaaaaacggc
    14641 ttccgtaacg ttataaatgt caatagccgc tatttccatc ctggtgggtg gcggcctccc
    14701 tacgtttaaa aaatggactt attatgaaaa agattaatgc aataatttta ttatcttctc
    14761 tgacctctgc ctcggtattt gctggcgcat acgtcgaaaa cagggaggca tataatcttg
    14821 cctccgatca gggcgaggtt atgctacgtg tgggttataa cttcgatatg ggcgcgggta
    14881 ttatgttaac caatacctac acctttcagc gagaagatga actaaaacat ggctataatg
    14941 aaattgaagg ttggtatcct ttattcaaac caaccgataa attaaccatc cagcccggtg
    15001 gcttaattaa tgataagagt atcagttcgg gtggtgcagt gtatctggac
//
LOCUS       DAEEUE010000092        15033 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.092, whole genome shotgun sequence.
ACCESSION   DAEEUE010000092 DAEEUE010000000
VERSION     DAEEUE010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15033
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.092"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..239
                     /locus_tag="KLK84_003108"
     CDS             <1..239
                     /locus_tag="KLK84_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HBE2010345.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            249..887
                     /locus_tag="KLK84_003109"
     CDS             249..887
                     /locus_tag="KLK84_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HBE2010346.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            890..2053
                     /locus_tag="KLK84_003110"
     CDS             890..2053
                     /locus_tag="KLK84_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBE2010347.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2137..3762
                     /locus_tag="KLK84_003111"
     CDS             2137..3762
                     /locus_tag="KLK84_003111"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HBE2010348.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(3879..4154)
                     /gene="yjfN"
                     /locus_tag="KLK84_003112"
     CDS             complement(3879..4154)
                     /gene="yjfN"
                     /locus_tag="KLK84_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HBE2010349.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4303..4632)
                     /gene="bsmA"
                     /locus_tag="KLK84_003113"
     CDS             complement(4303..4632)
                     /gene="bsmA"
                     /locus_tag="KLK84_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HBE2010350.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4814..5563
                     /gene="yjfP"
                     /locus_tag="KLK84_003114"
     CDS             4814..5563
                     /gene="yjfP"
                     /locus_tag="KLK84_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBE2010351.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5560..6315)
                     /gene="ulaR"
                     /locus_tag="KLK84_003115"
     CDS             complement(5560..6315)
                     /gene="ulaR"
                     /locus_tag="KLK84_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HBE2010352.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6423..7487)
                     /gene="ulaG"
                     /locus_tag="KLK84_003116"
     CDS             complement(6423..7487)
                     /gene="ulaG"
                     /locus_tag="KLK84_003116"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBE2010353.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7842..9239
                     /gene="ulaA"
                     /locus_tag="KLK84_003117"
     CDS             7842..9239
                     /gene="ulaA"
                     /locus_tag="KLK84_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBE2010354.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9255..9560
                     /gene="ulaB"
                     /locus_tag="KLK84_003118"
     CDS             9255..9560
                     /gene="ulaB"
                     /locus_tag="KLK84_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HBE2010355.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9570..10034
                     /gene="ulaC"
                     /locus_tag="KLK84_003119"
     CDS             9570..10034
                     /gene="ulaC"
                     /locus_tag="KLK84_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HBE2010356.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            10048..10698
                     /gene="ulaD"
                     /locus_tag="KLK84_003120"
     CDS             10048..10698
                     /gene="ulaD"
                     /locus_tag="KLK84_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBE2010357.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10708..11544
                     /gene="ulaE"
                     /locus_tag="KLK84_003121"
     CDS             10708..11544
                     /gene="ulaE"
                     /locus_tag="KLK84_003121"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HBE2010358.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSRERRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARMAKAGMVE
                     AA"
     gene            11544..12230
                     /gene="ulaF"
                     /locus_tag="KLK84_003122"
     CDS             11544..12230
                     /gene="ulaF"
                     /locus_tag="KLK84_003122"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HBE2010359.1"
                     /translation="MQKLKQQVFEANMDLPRYVLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     EIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12359..12634)
                     /locus_tag="KLK84_003123"
     CDS             complement(12359..12634)
                     /locus_tag="KLK84_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBE2010360.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            12961..13356
                     /gene="rpsF"
                     /locus_tag="KLK84_003124"
     CDS             12961..13356
                     /gene="rpsF"
                     /locus_tag="KLK84_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBE2010361.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13363..13677
                     /gene="priB"
                     /locus_tag="KLK84_003125"
     CDS             13363..13677
                     /gene="priB"
                     /locus_tag="KLK84_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HBE2010362.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13682..13909
                     /gene="rpsR"
                     /locus_tag="KLK84_003126"
     CDS             13682..13909
                     /gene="rpsR"
                     /locus_tag="KLK84_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBE2010363.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            13951..14400
                     /gene="rplI"
                     /locus_tag="KLK84_003127"
     CDS             13951..14400
                     /gene="rplI"
                     /locus_tag="KLK84_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBE2010364.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14471..>15033)
                     /locus_tag="KLK84_003128"
     CDS             complement(14471..>15033)
                     /locus_tag="KLK84_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HBE2010365.1"
                     /translation="TMNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDV
                     ESFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYIDQL
                     HTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRS
                     DRMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         3511 a         3667 c         4051 g         3804 t
ORIGIN      
        1 cattgttagg ttacgttatc ccattatcca gtgcggcaat caatgcggtg agtattccag
       61 actatttcgc ctggggcggg attgcactgg tgattcagtt actcgttttt gctggtgtca
      121 gactttatat gcccgcatta agcgaaaaaa ttattaatca caataccgca gcaggaatgt
      181 ttatgggaac cgccgcgctg gctggcggta tttttaacgc agcttgtatg acatggtaat
      241 ggacgatcat ggccagaaaa cgcaaatcaa gaaataacag taaaattggt cacggagcga
      301 tcagtcgcat tggtagaccg aataatcctt ttgaaccgcg tcgcaatcgc tacgcacaaa
      361 aatacttaac gttggcgcta atgggcggtg ccgctttttt cgtattgaaa ggttgtagcg
      421 atagtagcga tgtcgataac gacggcgacg gaacatttta cgcgacagtg caggattgta
      481 ttgatgacgg taataatgcc gacatttgcg cgcgtggctg gaacaacgcc aaagcggcat
      541 tttatgccga tgttccaaag aatatgactc agcagaactg tcagtctaag tacgaaaatt
      601 gctactacga caatgttgaa cagagttgga tcccggtcgt ttctggattt ttgttaagcc
      661 gggttattcg taaagatcgc gatgagccgt tcgtctataa cagcggcgga tcctcatttg
      721 cttcccgccc cgtctggcgc aacacttccg gtgattactc ctggcgcttc ggttctggca
      781 aaaaagagtc ttactcttca ggcggcttta ccaccagaaa agcgtcgacc gtttcgcgcg
      841 gcggctatgg tcgttcttcc agcgcccgtg ggcattgggg aggctaatca tgctgagaca
      901 caacgttcct gtgcggcggg atctggacca gatcgccgct aacaacggtt tcgactttca
      961 tatcatcgac aatgaaatct attgggacga gagtcgggct taccgtttta ctctgcgcca
     1021 gattgaagag cagatcgaaa aaccgactgc ggaactgcat cagatgtgcc ttgaggtggt
     1081 ggatcgcgcg gttaaagacg aagagatcct gacgcaactg gcgatcccgc cgttgtactg
     1141 ggatgtgatc gccgaaagct ggcgcgcacg cgatccttcg ctgtatggcc ggatggattt
     1201 tgcctggtgt ggcaatgcgc cggtgaagct gctggagtac aacgccgata cgccgacctc
     1261 gttgtatgag tcggcttatt tccagtggtt gtggctggag gatgcccggc gcagcggcat
     1321 tattccgcgt gatgccgatc agtacaacgc cattcaggaa cgcctgattt cgcgctttag
     1381 tgagctttac agtcgggaac cgttttattt ttgctgctgt caggacaccg atgaagacag
     1441 gagtaccgtg ctgtacttgc aggactgcgc ccagcaggca gggcaggagt cgcggtttat
     1501 ctacattgaa gatctcggtt tgggcgtcgg cggcgtactg accgatcttg atgataatgt
     1561 catccagcgt gcatttaagc tgtatccgct ggagtggatg atgcgtgacg ataacggtcc
     1621 gctgctgcgc aagcgtcgcg agcaatgggt ggagccgtta tggaaaagta tcttgagtaa
     1681 taaagggcta atgccgctgc tttggcgctt cttccctggt catcctaacc ttcttgcatc
     1741 ctggtttgag ggtgaaaaat cgcagattgc cgctggcgaa agctatgtgc gtaaaccgat
     1801 ctactcgcgc gaaggcggaa acgtcaccat tttcgacggt cagaataacg tcgttgacca
     1861 cgctgatggt gattacgccg atgaaccgat gatctaccag gcgtttcaac ctctgccgcg
     1921 atttggcgat agctacacac tcatcggtag ctggattgtc gatgatgaag cgtgcggaat
     1981 ggggatccgt gaagataaca cactgatcac caaagacacc tcacgtttcg ttccgcatta
     2041 cattgctgga taagaatgtt ttagcaatct ctttctgtca tgaatccatg gcagtgacca
     2101 tactaatggt gactgccatt gatggaggga gacacagtgc actggcaaac tcacaccatt
     2161 tttaatcaac ctataccatt aaataacagc aacttatacc tgtctgatgg cgcgctttgc
     2221 gaagcggtaa cgcgtgaagg tgctggctgg gatagcgatt ttctagccag tattggtcag
     2281 cagttaggaa cggctgaatc ccttgaactg gggcggctgg cgaatgtaaa cccgcctgaa
     2341 ctgttgcgct acgatgccca ggggcggcgt ctggacgatg ttcgtttcca tcccgcatgg
     2401 cacctgctga tgcaggcgtt atgtaccaat cgggtgcaca atcttgcctg ggaagaagac
     2461 gctcgctccg gcgcatttgt ggcgcgcgcg gcgcgtttta tgttacatgc gcaggttgag
     2521 gctgggtcgt tatgtccaat aacgatgacc tttgccgcca cgccattgtt gttacagatg
     2581 ttacccacgc cgtttcagga ctggaccacg ccgctgctga gcgatcgcta cgattctcac
     2641 ttattgccag gtgggcaaaa acgcggtttg ttgattggca tgggaatgac ggaaaagcag
     2701 ggcggttccg atgtcatgag caacaccacc cgtgcagagc gtttggaaga tggctcttat
     2761 cggctggtgg ggcataaatg gtttttctcg gtgccgcaaa gcgatgcgca tctggtgctg
     2821 gcgcagaccg cgggcggtct gtcctgcttt tttgtgccgc gctttttgcc tgacgggcaa
     2881 cgcaacgcga ttcgcctcga gcggctgaaa gataagctgg gtaatcgctc taacgccagt
     2941 tgcgaagtgg agtttcagga tgccattggc tggttattgg ggcaggaagg ggaaggaatt
     3001 cgtctgatcc tgaaaatggg ggggatgacg cgttttgatt gcgccctggg tagccatgcc
     3061 atgatgcgcc gtgcattttc gctggcgatt tatcatgcac atcaacgcca tgtttttggt
     3121 aatccattga tccaacagcc ccttatgcgt catgtcttaa gtcgcatggc acttcagctt
     3181 gaagggcaaa cggcgttgct gtttcgtctt gcgcgagcgt gggaccggcg tgccgatgcc
     3241 aaagaagccc tgtgggcgcg tttatttacg cctgcggcaa aatttgtgat ctgcaaacga
     3301 ggtatgccgt ttgtggccga agcgatggag gtgctgggcg gcattggtta ttgcgaggag
     3361 agcgagctgc cgcggcttta ccgggagatg ccggtaaaca gtatttggga aggttccggc
     3421 aatattatgt gcctggatgt gttgcgcgtt ctcaataagc aagcgggcgt atacgactta
     3481 ttgtcggaag catttgtgga agtgaaaggg caggatcgct attttgatcg cgcggttcgt
     3541 cgtttacagc agcagctgcg taagccagct gaagaactgg ggcgagagat tactcatcag
     3601 ctattcctgc tgggctgcgg tgcgcaaatg ttgaaatatg cttctccgcc aatggcgcag
     3661 gcgtggtgtc aggtgatgtt agatacgcgc ggcggcgtac ggttgtcaga gcagatccag
     3721 aatgatttat tgctgcgggc gacgggggga gtgtgtgtgt aagcgtatac gactgatgcg
     3781 acgctggttt cgattaacta aatgaaatat gtgaaaattg taggccggac aaggcgctcg
     3841 cgccgcatcc ggcattgttc atttgctgta gatgacactc atgcatacag tatcgcctgt
     3901 acgcgccagt tatcaggctg ctggtcttcg tacatggtaa taatgcggta ccatgacgca
     3961 ccttgttcat cggcctttaa cgccacgata cgttccacgt cctgcggatc ccctgaaata
     4021 ttgctaactg agatctgacc tatttcattc aggcccgtca cgcagtcagc actggcgaat
     4081 tctgcagact gtgccgtggc gctcgcagga taagtcacta attgcagcga gggtgaggca
     4141 agtaattgtt tcatcgtcag ctccattttt cgtttcccca catgttgcag ggcattccat
     4201 cgcggtaata aacgcccaag cttccagccg gtaatttatt gtgcacaggt aaacgcatag
     4261 ggacgcaaag caatgtaaag ctgactatgt aagttgtctg atttatttac ggtacaaaat
     4321 ggcttgtgaa taccactgtc ctgtcacgat ggtttcgtcc accatgacga caacgtaata
     4381 atcagctttt gcagcgacag cttttgctct gatttctgct aatgcgtcat ccggagaacc
     4441 ccgaaccatc gtgcttacgc tacctattcg ctgtaaccct tgcgtctggt cgcggcgaat
     4501 ctcttgcgga tggtccgtta ctggcggtgc tggctgtggc gtaccttgca gtgcgctaca
     4561 ggcacttaac atcaacacca ataataaact ggcaaaccgg taaataacgc tattacgttt
     4621 cctgctaacc atagtgtagt gccttattaa tttaactttg ggggaatgtt cccgaattgt
     4681 tacctggtac gcgattttcg aatgaaaacg tgacgaaagc gtaacccaca tctcaacatt
     4741 atgaactaat accgccagca gaaacttgtg ctaaggattt cttcatgtca taccagacaa
     4801 aaaggagaga cagatgattg aaatagaatc acgcgagctg gcagatattc ccgttcttca
     4861 tgcttatcct gtcgggcaaa aagatacccc gttaccgtgc gtaatttttt atcacggctt
     4921 tacttcatcc agcctggtgt atagctattt tgccgttgcg ctggcgcagg ctggtttgcg
     4981 ggtgatcatg ccggatgcgc ccgatcacgg tagccgtttt agtggcgacg aagcgcggcg
     5041 gttaaatcaa ttctggcaaa tcttgctaca aagtatgcag gaattcacta ctttacgtgc
     5101 ggcaatagcc gaagaaaact ggctgcttga tgaccgtctg gcagtcggtg gcgcgtcgat
     5161 gggcgcgatg acggcactgg gaattaccgc tcgccacccc acggtgaaat gtaccgccag
     5221 catgatggga tcgggctatt ttacatcgct cgcccgttca ctgtttccac cgctgatacc
     5281 tgaaacggca gcacagcaga atgaattcaa taaaattgtc gcgccgctgg cagagtggga
     5341 agcgacaaac cacctggaac aacttgggga cagacctcta ctgctgtggc atggcctcga
     5401 cgatgacgtt gtgcctgccg acgaatcact acgtttgcag caggccttaa gcgagacggg
     5461 acgggataaa ctgctaacct gttcatggca gccaggcgtg cgtcaccgta ttacgcctga
     5521 ggcgttagat gctgccgtga catttttccg ccagcatctt taaacacgca gaatgctgac
     5581 cccttgcgct tccagttgtt gcaggatttc cgggttagca tttttgccgg tgatcagcat
     5641 atcgatttga tcggcacggc taaaaagcat tcccgcgcgt tcgccaatct tactgctatc
     5701 aaccagtacc accagtttcc ctaccacgct cagcatcttc tgctctgcca ttgctgtcag
     5761 catatcggtt ttatacaacc cttctgcggt cagccctttt ccgctggtaa acatccagtg
     5821 cccggcatag agactgtttt cgctgccctg cgggcttaaa gtgatggact gacttttgtt
     5881 gtactgtcca cccataatga tcacgctgtc atgttcttga tcgatcaggt aatttgccag
     5941 cggtagataa ttagtgatga tttgcactgg cttgccacac atttcccgcc caagcagaaa
     6001 cgcggtggag ccgcagttga tgactacgct ttcgcccgga ttaaccagct gcgaggccgc
     6061 tttagcaata cgtacttttt catcgtgatt ctgcgcctga tgcagattca tcggcgtcca
     6121 gcgcgggcgc tgttgggtaa tagcttctgc gccattgcgc acctttttca gtttgccgct
     6181 ttcgccaagt ttattgatat cgcgtcgcgc agtggcaggc gaaattccca gacgctcaac
     6241 gactttctca acggtcacaa agcccaattg tgcgagcatt tccaggagga tttgatgtct
     6301 ttgtgcttca gtcatgagct attccgataa aatttgattt ttttagatga tatttgaaat
     6361 agccaggaaa tactacgccg gatagcgcga acttctccac gctatccggt ataaacgaca
     6421 gattacagga atgacttgaa cggcagatcc ggttcaatag tgaagcaatc atcgaaaccg
     6481 cgcggatagt ggtactcgaa gttgtcttta tccagcggcc aggtaaattt gccgccaacc
     6541 tgccagataa acggcttgaa gccatacttc agacgatctt ttttgatctc ccacagcacg
     6601 cggatctctt gcggatcggc ctggaagttt gaccagatgt cgtggtggaa cgggataacc
     6661 actttcgcat tcagggcttc acccatacgc agcatatcgg cgctggtcat tttgtcggta
     6721 ataccgcgcg ggttctcacc atacgatcct aacgccacat cgatctgatg ttcgttaccg
     6781 tgcttcgcat aatagttaga gtagtgggaa tcgccactgt gatataggga accgccaggg
     6841 gttttgaaca ggtagtttac tgcgcgatcg tccatgccat ctggcagtac gccagccgct
     6901 ttttgatcgg caggcagggt gatcagtgca gtacggtcga aagcgtcaag cgcatgaatt
     6961 tcaatgtctt tcacttttac aacatcgccc ggtttgacca cgatgcaacg ctctttcggt
     7021 acgccccagc caatccacaa atcaacgcag gttttcggtc caatgaatgg tacgtcatca
     7081 gcacagttct gcatcacggc agcagcgacg ttaacgtcga tatgatcgtt gtgatcgtga
     7141 gtcgccagta ccgcgtcgat ctggcgaatc gcaaacggat caagaacaaa cggggtggtg
     7201 cgtaggtttg gctgcagttt tttcacgcca gccatgcgct gcatctggtg accctgtttc
     7261 attaacgggt taccgtgact ttgtttgcca gtgccgcacc agaaatcaac gcaaacgttg
     7321 gtgccacctt ctgatttcaa ccagatcccg gtgcagccta gccaccacat cgcaaatgtg
     7381 ccaggggcga cctgctcttg ttcaatttct tcattcagcc agctacccca ctccgggaaa
     7441 gtgctcagga tccaggattc tcgggtgata cttttcactt tactcatcgc catttacctt
     7501 catgatagtt caattcgaat caacatgtga ttggttttga ttaatcctga cactattcct
     7561 tcaggaaggc aatgaccatt ttttgacttt ttgccaggga agttgttgtt gattttgagt
     7621 gtggaaagat ttaatggaat gtgtagttaa attaactaaa tgaatttaaa taaataattg
     7681 tttcgttgtg tgaatcacac tctatccgtg tggaattatt tgcgggtcgc gccacattta
     7741 atcataaata atcttgttgt gattactttt gaaaattaga gtgagtacac aacattccgg
     7801 gtgtgcggaa tacccggtta cctcttcttc aggagatcgt tatggagatc ctctacaaca
     7861 tctttaccgt gttttttaac caggtcatga ccaatgcccc gttgttgctg ggtattgtga
     7921 cctgtctggg ctacatccta ctgcgcaaaa gtgtcagcgt tattattaaa ggcacgatta
     7981 aaaccataat tggtttcatg ttgttgcagg cagggtccgg catcctcacc agcaccttca
     8041 aaccggtggt ggcgaaaatg tccgaagtct acggcattaa cggcgcaatt tccgatactt
     8101 acgcgtcgat gatggcaacc atcgaccgca tgggcgatgc ctatagctgg gtaggttacg
     8161 cggtgttgtt agcgctggcg ctgaacatct gttacgtgct gctgcgtcgc attaccggta
     8221 ttcgtaccat catgctgact ggtcatatca tgttccagca ggcggggctg attgccgtta
     8281 cgttgtttat tttcggctac tccatgtgga caaccattat ctgcacggcg attctggttt
     8341 cgctctactg gggcattacc tccaacatga tgtacaagcc gactcaggaa gtgacggacg
     8401 gctgcggttt ctccatcggt caccagcaac agtttgcatc attaattgcc tataaagttg
     8461 cgccgttcct cggcaaaaaa gaggaaagcg ttgaagacct caaactgcca ggctggctga
     8521 atattttcca cgacaacatc gtctccacgg cgattgtcat gaccatcttc tttggtgcca
     8581 ttctgctctc cttcggtatc gacaccgtgc aggcgatggc aggcaaagtg cactggacgg
     8641 tctatatcct gcaaaccggt ttttccttcg cggtggcgat cttcatcatc actcagggtg
     8701 tgcgcatgtt tgtggcggaa ctctctgaag cgtttaacgg tatctcccag cgcctgatcc
     8761 ctggtgcggt tctggcgatt gactgtgcgg ctatttatag cttcgctccg aacgccgtgg
     8821 tctggggctt tatgtggggc accatcggtc agctgattgc ggttggcatc ctggtcgcct
     8881 gcggttcctc gatcctgatt atcccaggct ttatcccgat gttcttctcc aacgccacca
     8941 tcggcgtgtt cgctaaccac ttcggcggct ggcgtgcggc gctgaagatt tgtctggtga
     9001 tggggatgat cgaaatcttt ggttgcgtct gggtggtgaa acttaccggt atgagtgcct
     9061 ggatggggat ggcggactgg tcgattctgg caccgccgat gatgcagggc ttcttctcca
     9121 tcggtatcgc ctttatggcc gtcatcattg taattgcact ggcttatatg ttcttcgctg
     9181 gccgcgcact gcgcgcagaa gaagatgcag aaaaacaatt ggcagaacag tctgcttaat
     9241 aaggagtttt gattatgacc gtacgtattc tggctgtgtg tggcaacgga caaggcagtt
     9301 ccatgatcat gaagatgaaa gtggaccagt ttttaaccca atcaaacatt gaccacacgg
     9361 taaatagctg cgcggttggc gaatacaaaa gcgagttgag cggcgcggat atcatcatcg
     9421 cttctacgca cattgcgggc gaaatcaccg tgaccggcaa caaatacgtg gttggcgtgc
     9481 gcaacatgct ctctcctgcc gactttggcc cgaaactgct ggaagtgatc aaagcgcatt
     9541 tcccgcagga tgtgaagtaa ggacgcgcca tgaaattaca tgattcgctg gcggagaata
     9601 aatccatccg cctgcaggct gaagctgaga catggcagga cgcagtaaaa atcggcgttg
     9661 acctgctggt ggcggcggat gttgtcgagc cgcgttacta ccaggcgatt ctggatgcgg
     9721 ttgaacagca cggtccgtac tttgtgctcg caccaggcct ggcaatgccg cacggtcgcc
     9781 cggaagaggg cgttaagaaa accggttttg cgctggtgac gctgaaaaag ccgctggagt
     9841 tcaaccacga agataacgac ccggtggata tcctcatcac catggcggcg gtcgatgcca
     9901 acacgcacca ggaagtgggc atcatgcaga tcgtcaacct gtttgaagat gaagaaaatt
     9961 tcgaccgttt acgtgcctgc cgtaccgagc aggaagtact ggatctcatt gaccgcacca
    10021 acgcggcagc ttaagaagga attgaagatg tcattaccga tgttgcaagt cgcgctggac
    10081 aaccagacta tggatagcgc ctacgaaacc actcgcctga ttgccgaaga agtcgacatt
    10141 atcgaagtgg gtaccattct gtgcgtgggc gaaggcgtgc gtgcggttcg cgatctgaaa
    10201 gcgctctacc cgcacaaaat cgtgctggca gacgccaaaa ttgccgatgc gggcaaaatt
    10261 ctttctcgta tgtgcttcga agccaacgct gactgggtga ccgtgatttg ttgtgcggat
    10321 atcaacaccg ccaaaggtgc gctggacgta gcaaaagagt ttaacggcga cgtgcagatc
    10381 gaactgaccg gttactggac ctgggaacag gcgcaacagt ggcgcgacgc aggtattcag
    10441 caggtggttt atcaccgcag ccgtgacgcg caggccgctg gcgtggcgtg gggtgaagcc
    10501 gatatcactg cgatcaaacg tctttccgat atgggcttca aagtcaccgt caccggtggc
    10561 ctggcgctgg aagatctgcc gctgttcaaa ggcattccaa ttcatgtgtt tatcgccggt
    10621 cgcagcatcc gtgatgccgc ttctccggtt gaagccgcgc gtcagttcaa acgttccatc
    10681 gccgaactgt ggggctaagg agcggatatg ttgtccaaac aaatcccgct tggcatctat
    10741 gaaaaagcgc tccccgccgg ggagtgctgg ctggaacgcc tgcgactggc aaaaacgttg
    10801 ggcttcgatt ttgtcgagat gtcggtagat gaaactaacg agcgcctgtc gcgcctcgac
    10861 tggagccgcg aacggcgtct ggcgctggtg aatgctattg ttgaaaccgg cgtgcgtgtg
    10921 ccgtccatgt gcctttctgc ccatcgtcgt ttcccgttgg gcagcgaaga tgacgcggtg
    10981 cgggcgcagg ggctggagat tatgcgtaaa gccatccagt tcgcccagga cgttggcatt
    11041 cgcgtgatcc agctggcggg ctatgacgtt tactatcagg aagccaataa cgaaacgcgt
    11101 cgtcgtttcc gtgacggcct gaaagagagc gttgagatgg cgagccgcgc gcaggtgacg
    11161 ctggcgatgg agatcatgga ttatccgttg atgaactcca tcagcaaggc gctgggttac
    11221 gcgcactatc tcaacaaccc gtggttccag ctctacccgg atatcggcaa cctgtcggcg
    11281 tgggacaacg atgtgcagat ggagttgcag gccggaatcg ggcatatcgt cgcggtgcat
    11341 gtgaaagaca ccaaaccggg cgtcttcaaa aacgtgccat ttggcgaagg tgtggtggat
    11401 ttcgaacgtt gcttcgaaac gctcaaacag agtggctatt gcgggccgta cctgattgag
    11461 atgtggagcg aaacggcgga agacccggcg gcagaagtgg tgaaagcgcg catggccaaa
    11521 gcaggcatgg tggaggcggc ataatgcaaa agctaaaaca gcaggtgttt gaagccaaca
    11581 tggatctgcc gcgctatgtg ctggtgacct ttacttgggg caacgtcagc gctatcgacc
    11641 gcgaacgcgg gctggtggtg atcaagccca gcggcgttgc ctacgaaacc atgaaagcag
    11701 acgatatggt ggtggtcgat atgagcggca aggtggtgga aggggagtat cgcccatctt
    11761 ccgacactgc gacgcatctc gaactctacc gtcgttaccc gtcgcttggc ggcattgtcc
    11821 atacccactc cactcatgcc accgcatggg cgcaggcggg gctggcgatc ccggcattag
    11881 gcaccacgca cgctgactac ttctttggtg atatcccgtg tacgcgtgga ttaagcgaag
    11941 aagaggtgca gggtgagtat gaactgaaca ctggcaaagt gattatcgaa acgctgggca
    12001 acgccgagcc gctgcatacg ccggaaattg tggtgtatca gcacggaccg ttcgcctggg
    12061 ggaaagatgc ccacgacgcg gtgcataacg cggtagtgat ggaagaagtg gcgaaaatgg
    12121 cgtggattgc gcgcagtatt aatccacaac tcaatcatat cgacagctac ctgatgaata
    12181 aacacttcat gcgtaagcac ggtcctaacg cttattacgg gcagaagtaa aacacgcgct
    12241 gcggaaattt ccttcctcgg gagataactg gtctaattcc gcagccgttt ttcaaaaaaa
    12301 gccccctgcg aagggggcaa agcaaactat ggcaatgttt cgttggttat acctggtgct
    12361 agcgataaat atccgcgctg gcgtgcatat tgccgttact ccccggttcc cgcatcagaa
    12421 ttacgtggta gtacgttgcg ccttgcgcat ctgtttcttc atttaatgcc tgacgtgctt
    12481 cgctttcagt ggcgaaatta tgattgatat aaatcacgcc taagctttgc acatcgtcca
    12541 tatttctggc ctggtggtta ttaatttcaa tggctgccca tgtatttgca cttagcaaaa
    12601 gcacagccag aagggctaaa actcgactga acatagatac ctcctcgacg gctgactttg
    12661 tgtgctctcc tctgtgatga tcttctgatt taattttaat caatgataaa gaagttgatg
    12721 gtgaccattt ctgatgcagt tgttcaaaaa aacactgtga cggaatgtga tgaacttcaa
    12781 atcagcggtt tagaggttat ttgcgaaagg ggagatttat ttctgctccg cccttgagtt
    12841 tagcggggta tgccagtact ataacgcgtc atttttcagc cgacctttaa cacgttcctt
    12901 gcctccccgg gattcggctg acccagacag gaggctgaat aatccgtaag gagcaattcg
    12961 atgcgtcatt acgaaatcgt ttttatggtc catcctgacc agagcgaaca ggttccgggc
    13021 atgatcgagc gctacactgc tgccatcact ggtgcagaag gcaagatcca ccgtctggaa
    13081 gactggggcc gccgtcagct ggcttacccg atcaacaaac tgcacaaagc acactacgtt
    13141 ctgatgaacg ttgaagctcc gcaggaagtg atcgatgagc tggaaactac cttccgcttc
    13201 aacgatgccg ttatccgcag catggttatg cgtaccaagc acgctgttac cgaagcatct
    13261 ccgatggtta aagcgaaaga cgagcgccgt gagcgtcgcg atgatttcgc aaacgaaacc
    13321 gctgatgatg ctgaagctgg ggattctgaa gagtaatttc tgatgaccaa ccgtctggtg
    13381 ttgtccggca ccgtgtgcag gactcccctt cgaaaggtca gtccatcagg aattcctcac
    13441 tgccagttcg tgcttgagca tcgttctgtg caggaggaag ccggctttca ccggcaggca
    13501 tggtgtcaaa tgcccgttat tgttagcgga cacgaaaacc aggccattac tcacagtata
    13561 acggtcggca gtcgcataac cgttcagggg ttcatttcat gccacaaggc aaagaacgga
    13621 ctgagcaaaa tggttttgca tgccgagcag attgaattga tagattctgg agactagcca
    13681 tatggcacgt tatttccgtc gtcgcaagtt ctgccgtttc accgcggaag gcgttcaaga
    13741 gatcgactat aaagatatcg ctacgctgaa aaactacatc accgaaagcg gtaagattgt
    13801 cccaagccgt atcaccggta cccgtgcaaa ataccagcgt cagctggctc gcgctatcaa
    13861 acgcgctcgc tacctgtccc tgctgccgta cactgatcgc catcagtaat cggtcacggt
    13921 ccattaatac gactttgaga ggataaggta atgcaagtta ttctgcttga taaagtagca
    13981 aacctgggta gcctgggtga tcaggtaaac gttaaagcgg gctatgctcg taacttcctg
    14041 gtaccgcagg gtaaagctgt tccagctacc aagaaaaaca ttgaattctt cgaagcacgt
    14101 cgcgctgaac tggaagctaa actggctgaa gttctggcag ctgctaatgc tcgcgctgag
    14161 aaaatcaatg cactggaaac tgttaccatc gcgtctaaag ctggcgacga aggtaaactg
    14221 ttcggttcca tcggtactcg cgacatcgct gacgctgtaa ctgcagctgg cgttgaagtg
    14281 gctaagagcg aagttcgtct gccgaacggc gttctgcgta ccactggcga acacgaagtg
    14341 agcttccagg ttcacagcga agtattcgcg aaagtgatcg taaacgtagt agctgaataa
    14401 ttcgttattc aacgagacgt aaaaacgccg accattggtc ggcgttttgc tttctatttt
    14461 tcgtcaggca ttaatttcgc aagtagatcc attcaccatt ctcttgtgtc cataagtcag
    14521 gatggtttgc tggcgttaga tgcatacgat cactccttat gtctcggtcc tgcggcactc
    14581 gctcgaaacc taaacccgag tagattgcgg ctgaatttga taaggaacgt agaccaattt
    14641 tcaaatcatg gggtaaatca gaatacgcgt ttagcaatgc agcaatgatg aatccgaggg
    14701 cattagaaac gcctgtaaca gtgtgaagtt ggtcgatata aagatcgatt gttggtagtt
    14761 cagatgacca gcaattaggc atttgggcag gaagggttac aacgtatcct atcgtttggc
    14821 cggtatagtc tggagtacta taaccaggtc ccaaaacaac ctcgtgtatt gtgtatttac
    14881 tgttgggttc gaaggattcg acatcttgca ataaatattt cagttctgcg gttaacctcg
    14941 cacccgcgta agctttattg tatttctggg cgtgaatatt atctaatgta gcttcatggt
    15001 tccatccaaa agaattatca ccattcattg tta
//
LOCUS       DAEEUE010000093        14920 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.093, whole genome shotgun sequence.
ACCESSION   DAEEUE010000093 DAEEUE010000000
VERSION     DAEEUE010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14920
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.093"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(100..525)
                     /gene="arsC"
                     /locus_tag="KLK84_003129"
     CDS             complement(100..525)
                     /gene="arsC"
                     /locus_tag="KLK84_003129"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HBE2010366.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(538..1827)
                     /gene="arsB"
                     /locus_tag="KLK84_003130"
     CDS             complement(538..1827)
                     /gene="arsB"
                     /locus_tag="KLK84_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HBE2010367.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1881..2234)
                     /gene="arsR"
                     /locus_tag="KLK84_003131"
     CDS             complement(1881..2234)
                     /gene="arsR"
                     /locus_tag="KLK84_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HBE2010368.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            2977..3060
                     /locus_tag="KLK84_003132"
     CDS             2977..3060
                     /locus_tag="KLK84_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HBE2010369.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3114..4466)
                     /gene="gorA"
                     /locus_tag="KLK84_003133"
     CDS             complement(3114..4466)
                     /gene="gorA"
                     /locus_tag="KLK84_003133"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBE2010370.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4538..5380)
                     /gene="rlmJ"
                     /locus_tag="KLK84_003134"
     CDS             complement(4538..5380)
                     /gene="rlmJ"
                     /locus_tag="KLK84_003134"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HBE2010371.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5583..7625
                     /gene="prlC"
                     /locus_tag="KLK84_003135"
     CDS             5583..7625
                     /gene="prlC"
                     /locus_tag="KLK84_003135"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HBE2010372.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            7633..8385
                     /gene="rsmJ"
                     /locus_tag="KLK84_003136"
     CDS             7633..8385
                     /gene="rsmJ"
                     /locus_tag="KLK84_003136"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HBE2010373.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8434..9903)
                     /gene="dtpB"
                     /locus_tag="KLK84_003137"
                     /pseudo
     CDS             complement(8434..9903)
                     /gene="dtpB"
                     /locus_tag="KLK84_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(10221..10655)
                     /gene="uspA"
                     /locus_tag="KLK84_003138"
     CDS             complement(10221..10655)
                     /gene="uspA"
                     /locus_tag="KLK84_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HBE2010374.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11049..11384
                     /gene="uspB"
                     /locus_tag="KLK84_003139"
     CDS             11049..11384
                     /gene="uspB"
                     /locus_tag="KLK84_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HBE2010375.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11455..12954)
                     /gene="pitA"
                     /locus_tag="KLK84_003140"
     CDS             complement(11455..12954)
                     /gene="pitA"
                     /locus_tag="KLK84_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HBE2010376.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            13186..14385
                     /locus_tag="KLK84_003141"
     CDS             13186..14385
                     /locus_tag="KLK84_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBE2010377.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3713 a         3789 c         3928 g         3490 t
ORIGIN      
        1 gaatttgcac ggttttgtag gtcggataag acgcgttagc gtcgcatccg acataaacaa
       61 agcgcacttt accaactatc ggcagtggat atcgccgctt tatttcaggc gcttacccgc
      121 ttcatcaacc actttctcgc catcttcctt ggagaatgcg cctttttgcg catctggcag
      181 aatttccagc accacttctg aagggcggca caggcgagtt cccagcggcg tcaccacaat
      241 cgggcgatta atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa
      301 tttatcttcc gcaaggccca gctcctcata cggttcgacg tttttacgca gcagcgcgcg
      361 tacggaaatc cccatatcgg caatgagttt gaccagttca tcgcgcgttg gcggagtttc
      421 cagataatgg ataatggtcg gttctgtgcc gctgttgcgg atcatctcca gcgtattacg
      481 cgacgtgccg caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta
      541 caaagtgaaa gagagacgta gcgccagcgc agccagcgtc acaaacagca caggcagggt
      601 catgataatc cctgtacgga agtaatatcc ccagctgata gtcatattct tctgcgaaag
      661 tacgtgcagc cagagcagcg ttgccaggct accaatcggg gtaattttcg gccccaaatc
      721 gcagccaatc acgttggcat aaaccatcgc ttctttgata acgccagatg ccgtgctgcc
      781 atcaatggac aacgcgccaa ccagtaccgt cggcatattg ttcataatag aagagaggaa
      841 ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt
      901 gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc aggccataaa ccaccagata
      961 catgccgagc gagaagatga caatctgcca gggggcaccg cgcaggactt tacccgtatt
     1021 aatcgcatga ccgcgtttag cgacgacaaa taatatcagt gcgcccacag ctgcaatggc
     1081 gctcaccgga atgccgagcg gttccaagac gaaaaatccc accagcagaa gcagtaaaac
     1141 aacccagcca gttttgaacg tagcaggatc tttgatcgct tctgcgggag atttcagcag
     1201 cgccatatcg tagttctgcg gaatatcttt gcgaaaatag agatgtagca tcaccagcgt
     1261 ggcaacaatc gcggcgatat ccaccggcac catcaccgag gcgtattcgc gaaagccgag
     1321 gccaaagaaa tcagcggaaa cgatattcac caggttggag acaataagcg gcaggctggc
     1381 ggtatcggca atgaatccgg ccgccatcac gaacgccagc gtcgtgcctt tactgaatcc
     1441 taaagccagc agcatggcga tgacaatcgg cgtcaaaata agcgccgcgc catcattggc
     1501 aaacagggcc gcaacggcgg caccgagcag gacaatccag gtaaacagca agcgaccgcg
     1561 tccattgccc cagcgtgaaa cgtgcagcgc tgcccattca aaaaagccgg actcatccag
     1621 cagcaggctg atgataatga cggcaataaa cgccgccgtc gcgttccaga cgatattcca
     1681 caccaccgga atatcacccg gatgaaccac gcccgtaacc aacgccagta ccgcgccgag
     1741 cgttgcactc cagccgatgc ctaaaccttt cggctgccag ataaccaata cgatggtcag
     1801 gacaaagata gcgcctgcca gtaacataat gcctcccgga taaaacacat gtgcaaatcc
     1861 atatgtgttt agctaaattt ttaactgcaa atgttcttac tgtctccgga acagttttgt
     1921 ctggccaggt tgcggacaat tgcctgaatc ttttcctgtt cacatcgcca ggcttcatca
     1981 ataattttcg ccgcccatgc tggaatatgc ggcgataagc ggtaatgaac ccacttacct
     2041 tgcttgcggt ccagcaatag cccgctttca cgcagcaatg ccagatggcg ggatatcttg
     2101 ggctgcgact gatcgagggc agtgcagaga tcgcagacgc ataactctcc cagttcgcta
     2161 agcagtaaaa cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatgggt
     2221 aacagaaatg acatattgcg ctcctgattg ttgcaggtag tgtctctctt cgaagcggat
     2281 aagtcaaaaa catatatgac ttaacgaatg tgtaagtgca gaggaaggta ataggtgtga
     2341 attttgagtt ggctattcat ttgaaaggcg gtttgaatca ggacgtaaaa aaaccgactt
     2401 tgcgtcggtt ttttactctt cagcctgagt tggtggctct ggctggagtg agaatgccca
     2461 catctaacgg ttcaatacta ttagccagtg gctaacattg caagcgttag cgatttcttc
     2521 agctggagaa gggacagttt gctatacatg gtggtgaaag aggatttata tactttcatc
     2581 gggtcgctgc cgtaccaagc cttgtccgaa tccggacgta aaaaaaccga cttcgcgtcg
     2641 gtttttttac tcttcagccc agagttggtg gctctggctg gagtgagaat gccatcatct
     2701 aacgcatcaa tgctaatacc atactgaaac tattgcaagg ttatgctggt tatttagcct
     2761 aattacactg tatgattatc cagttagctc tgaggcattt tcactctggc aatgcgcata
     2821 aacgctttca aagtcctggt cagaagtacg ggtggtgccg ttaactgatg ctctggccgg
     2881 agtgagattg cccacatcta acaatgaggc atgcgccgtg acaggcagtg gatgagtaag
     2941 cggatgcatt ctcactccat cgcatggaga aaacgggtga ttgataaagc aatcatcgtt
     3001 cttggggcgt taattgcgct gctggaactg atccgctttc tgcttcagct tctgaactga
     3061 tagcggaaac gtaattaagg gctaagagca cactactctt agccctttaa catttaacgc
     3121 atggtcacga actcttctgc cgccgttggg tgaatggcga cggtattgtc gaagtctttt
     3181 ttggttgcgc ccatcttcag cgccaccgcg aagccctgca acatttcgtc cataccaaag
     3241 ccaatgccgt gaataccgac aatcttctct tctggcccaa cgcacaccag cttcatgcgg
     3301 cacggctggc ggtgagtggt gacggcggta tacatcgcgg tgaaagagga tttatacact
     3361 ttcacctgat cgtcgccgta ctgctcgcgc gcctgcggtt ccgttaaacc aacagtacca
     3421 atcggcggat ggctgaagac cacggtcgga atgttgctgt aatccagatg ctcatccggc
     3481 ttgttattaa acaggcgttc agagagacgg cgacccgctg caactgccac cggtgtcagt
     3541 tccactgcac ccgtgttatc gcccaccgcg taaatacctt caatattggt gttttgatat
     3601 ttatcgacga cgatatagcc tttttcgttg gttttaacgc cagcggcttc gaggttgatg
     3661 ttgtcattgg caggctcgcg accaatcgcc caaatcaggc aatccaccgt ttcactgcga
     3721 ccatcttcca gctccagcgt caggctacca tcggcatttt tcactaccgc tttcgggatg
     3781 gcgttggtat gcaactgtgg gccttcggcg ttcatcactt cgaccagcgt ttcagagatc
     3841 atcgggtcga agctgcgcag cggcgcatgt ttacgcacaa acagatgcgt tttcgcgccg
     3901 aggccgttaa tcacgccagc cagctcaacg gcgatgtaac ccgcgccaac aaccgccacg
     3961 cgctctggca aagcaggaag ggcgaagaag ccatcggaat caataccgta ttccacgcct
     4021 ggaatatccg ggtggctcgg acgaccgccg gtggcgatca gaatatgatc ggccgtgatg
     4081 gtttcgccgt taacttccag cgttttggca tcaacgaagc gggcaaagcc tttgattaca
     4141 tcaacgttat ttttaccgag cacgttttca taggaagtat gaatacggtc gatataggcg
     4201 gtacggctgg cgatcaacgt ttcccagttg aacttgttga tggtggtatc gaaaccataa
     4261 tcagggccgt acatatggat cgcttcacgg atttgcgccg cgtgccacat cacttttttc
     4321 ggcacgcagc cgacgttcac gcaggtgccg cccagctctt tggcttcaat cagtgcgcat
     4381 ttctggccgt acatagccgc gcggttgatg gaggcgatac cgccgctgcc gccgccgatg
     4441 gcgatgtaat catagtgttt agtcatgaca tattgtcctt atcgttgatt accgcgattg
     4501 tagcgcgaga cgtaataggt gccagcaatg gctgcaatta ctccggcacg atccagctta
     4561 cggtggcgtg cccggtacct gccggaacca gtttgctgtg cagccacgac agcacgttat
     4621 tcatctgctg ttccagcttc cacggcgggt taatcacaat catgccggaa gcggtcatgc
     4681 cacggcgatc gctgtctggc agcaccgcca gttcaatttg cagaattttg cgaatgccgg
     4741 tcgcttccag gtcgtggatc atgcgcttaa tttgctggcg cagcaccacc ggataccaca
     4801 atgcgtaagt gccagtggcg aaacgtttgt aaccttctgc tatcccgctg accaccgcct
     4861 gatagtcggt tttcatttca tacggcgggt cgataaggat taaaccacgg cgagaaaccg
     4921 gcggcagttt ggccttaagt tgctggaaac cgtcggcttt ttcgacgcgt gcacggctgt
     4981 ctttctgaaa tccagaacgc agcaacgggt aatcgctcgg gtgcagttcg gtcagttgca
     5041 ggctgtcttg ttcgcgcagt agctggcgag caatcaacgg cgaaccgggg tagtaacgca
     5101 actggccgct acggttgaag tgttttacca cattgatgta cgcctccagt tctgcgggca
     5161 aatcgtcctg ctgccagata cgggcgatac cttcgagata ttcgccggta cgctcggcat
     5221 gttcgctacc taactgataa cgcccggccc ctgcgtgggt gtcgagatag agaaacggtt
     5281 tatctttctc tttcagcgac tcgatgatca ggctctgaac ggtatgttta aggacgtcgg
     5341 cgtggttgcc agcgtgaaag ctgtggcgat aactgagcat gggtaaaggt gttccggtaa
     5401 gtaaaaattg gcccgtattc ggggcgcaca aaagcctatc aggacagtat accgaaaaga
     5461 ggccgccgcc gcgaaagcgt aacgtttctc attgaaattc actacactta accccatgct
     5521 acacacatta tgtaaagcgc ctgttgagcg cttccttaac ctctttaacc aggactgcgc
     5581 taatgacgaa tccgttactg actccctttg aattgcctcc gttttctaaa attctcccgg
     5641 aacatgtcgt tccagccgtg actaaggcat tgaacgactg ccgcgaaaat gtggagcgcg
     5701 tagtagcgca aggggcaccg tacacctggg aaaatctctg ccagccgttg gcggaagtgg
     5761 acgatgtttt ggggcgtatc ttctccccgg tcagccacct gaactcggtg aaaaatagcc
     5821 cggaactgcg tgaagcgtac gaacaaaccc tgccgctgct gtcggaatac agcacctggg
     5881 tagggcaaca tgaagggctg tataaagcgt accgcgacct gcgcgatggc gatcattacg
     5941 ccacgctgaa cacggcgcag aagaaagcgg ttgataacgc cctgcgtgat tttgaactct
     6001 ctggcatagg tctgccgaaa gagaaacagc agcgttacgg cgaaattgct acccgtcttt
     6061 ctgaactggg caaccagtac agcaacaacg tcctcgatgc gacgatgggc tggaccaaac
     6121 tcgttaccga cgaagcgggg ctggcgggga tgccagaaag cgcgctggct gcggcaaaag
     6181 cccaggccga agcgaaagag ctggaaggct acctgctgac gctggatatc ccaagctacc
     6241 tgccggtaat gacctactgc gacaaccagg ctctgcgcga agagatgtat cgcgcttaca
     6301 gcacccgcgc ctccgatcaa ggcccgaatg ccggtaagtg ggataacagc aaggtgatgg
     6361 aagagatcct cgcgctgcgt cacgaactgg cgcaactgct gggctttgaa aactacgcct
     6421 ttaaatccct tgccactaaa atggcagaaa acccgcagca ggtgctggat ttcttaaccg
     6481 atctggcaaa acgcgcgcgt ccgcaaggcg aaaaagagct ggagcaactg cgtgccttcg
     6541 ctaaagccga atttggtgtc gatgagttgc agccgtggga tatcgcttac tacagcgaaa
     6601 aacagaaaca gcacctctac agcatcagcg acgagcagct gcgtccgtac ttcccggaaa
     6661 acaaagcggt taacggcctg tttgaagtgg tgaaacgtat ttacggcatc accgctaaag
     6721 agcgtaaaga tgttgatgtc tggcatccgg atgtacgttt cttcgaactg tatgacgaaa
     6781 ataacgaact gcgcggcagc ttctacctcg atctgtatgc ccgtgaaaac aagcgcggcg
     6841 gggcgtggat ggatggctgc gtaggccaga tgcgtaaagc tgatggttct ctgcaaaaac
     6901 cggtcgcgta tttgacttgt aacttcaacc gcccggtaaa tggtaaaccg gcgctgttca
     6961 ctcacgacga agtgatcacc ctgttccacg agttcggtca cggcctgcac catatgctga
     7021 cccgcatcga aaccgctggt gtttccggta tcagcggtgt gccgtgggat gcggtcgaac
     7081 tgccgagtca gtttatggaa aactggtgct gggagccgga ggcgctggcg tttatctctg
     7141 gtcactatga aaccggcgaa ccgctgccga aagagttgct ggataaaatg ctggcggcga
     7201 agaactacca ggcggcgctg tttattctgc gtcagctgga gttcggcctg tttgatttcc
     7261 gccttcatgc cgagttccgc ccggatcagg gggcaaaaat cctcgaaact ctggcagaaa
     7321 tcaagaaact ggttgccgtg gtgccatctc cgtcctgggg ccgtttcccg cacgctttca
     7381 gccatatttt cgccggtggt tatgcggcag gttactacag ctacctgtgg gctgacgtac
     7441 tggcggcaga tgctttctcg cgctttgagg aagagggcat tttcaaccgt gaaaccgggc
     7501 agtcgttcct cgacaacatt ctgagccgtg gcggttcaga agagccgatg gatctgttca
     7561 aacgcttccg tggtcgtgaa ccgcagctgg atgcgatgct ggagcattac ggcattaagg
     7621 gctgatcatt cagtgaaaat ctgcttaatt gatgaaacag gcgccggaga cggcgcctta
     7681 tctgttctgg cggcccgctg ggggctggag cacgatgaag acaacctgat ggcgctggtg
     7741 ttaacgccgg aacatctgga attgcgcaag cgtgatgagc caaaacttgg cggcatcttt
     7801 gttgattttg ttggcggagc gatggcgcac cgacgcaaat tcggcggtgg tcgcggtgag
     7861 gcggtggcga aagcggtggg cattaaaggc gattatttgc cggatgtggt ggacgccacc
     7921 gcagggctgg ggcgcgatgc ctttgtactg gcttcagtcg gctgccgcgt gcggatgctg
     7981 gagcgtaatc cagtggttgc cgcgctactc gacgacggcc tggcgcgtgg ttatgcggat
     8041 gcggaaatcg gcggctggtt gcaggagcgg ttgcagttaa ttcacgcctc cagcctgacg
     8101 gcgctaaccg atattacccc acgcccgcag gtggtctatc tcgatccgat gttcccgcat
     8161 aagcagaaaa gcgcgctggt gaaaaaagag atgcgtgtct ttcagtcgct ggtggggccg
     8221 gatcttgatg ccgatggatt actggagcct gcacgcttgc tggcgaccaa acgcgtggtg
     8281 gtaaagcgcc cggactacgc gccaccgctg gcgaatgtcg ccacgccaaa cgcggttgtg
     8341 actaaagggc atcgctttga tatctatgca ggtacgccag tgtaaaagta aagcagggta
     8401 gcgataacgc taccctgctt tctgcaagaa taattaatgg ctttccggcg tcgcaatcat
     8461 gcgtttcagc cacggcacca tcaacagcat cactactgca acgcccagcg tgaccagacc
     8521 aatcttacca aacacgttgg tatagacggg caacgtctca agcggatcgg taatgttgtc
     8581 cggcaccgcg gtaaatgttg ccacatagcc gcccagcaag aacgcggcag cctgcgtcag
     8641 gaaccacatc ccgagaataa agcccatcaa atgctgcggc accagggcag caatcatcgc
     8701 caggccaagg gcgctaataa acaattcacc caggctctag aataaataca ccagcacgat
     8761 aaaccatggc gatgtcagcc cctggtcatc cgcaaaccac attcccgcag cagcagccgt
     8821 caaaaagccc agtgagcaca taaacatgct gagagtaaat ttcatcggca tcgagaggtc
     8881 tttgcctttg ttacccagat gcgtgtaaat gcctgccagt attgggctgg cgagtaccac
     8941 ccagaacggg ttaagcgcct ggaagctgac cgggttgatg gaaaaaccga gaatttcatg
     9001 atgcacgttg ttgatggcaa agaagttcag cgatgttggc atctgggcgt agagaatgta
     9061 aaacaccacc gcttcgagca tcaggacaaa ggcgacaaac attttattgc gcccggtttt
     9121 atccagcttg aatgcctgac gaaagaagat gatagtgacg acgatggaga gaacaatcag
     9181 caccagattg gcgacttcta cgttgtgcat cagccatgca catacgaaga tcatcaccac
     9241 gctgccaagt aatacgtaca acagtttgct gaagctcatc ggcttgaagt cgggttcaga
     9301 accaatgtct ttcaccattc cacgacaggc gatgtaaacc agtaatgcga taattaaccc
     9361 cgccccgcac aggttgtagg tgactgaata accgaatcta tcagcgatca caggggccag
     9421 cgataacgct atcaacgagc cgatattgat cgacatatag aacagggtga atgcgccatc
     9481 aagccgcgga tctttcggcg gatagcactt cgaaagcaag ctggctgggt tagctttaaa
     9541 caggccgtta ccgacagcga tagtccccag ggcgatgaaa atcaggtcag gcttaagtag
     9601 cgacatgccg gtcatgaagt agccaatcgc cagcacaagt gctccaagaa caatggtgcg
     9661 tttggtcccc aacaggtggt cgccgacata gccgccaatg gaaatgaggc catagaccag
     9721 tgcagcaaaa gcaccaaaag tgacaaaagc ctgctcttgc gagaatccaa gctgtttaac
     9781 gaagaaaacc gccagtacgc cctgcacgcc gtagtagccg aatcgctccc ataactcgac
     9841 aaaaaagatc atgaaaaatg ggcgaggttg ctgcagcatc cccatgggtg ttgttgtatt
     9901 aatatttatt aaccttccaa taccatcctg aaaagtatga acgcgtaagg gataacgcgc
     9961 gaaaaagttt acatctgtca gagattctta atattgatgc ggtaaataga ttaatacact
    10021 attacaacag aaaataacca gctgatcaca ctaatgatgg aaaatatgat ggtgttaaat
    10081 tttgtattgg caggagattt tgattgcgta cgcagatcgt aggccagata aggggtttac
    10141 gctgcatcag gtaacaaggc gcgccaaaaa gcaaaacgcc cgcatatgcg ggcgttcagg
    10201 aacacgtcgt ggagggatga ttattcttct tcgtcgcgta gcggaacaat cagcatatca
    10261 acgtgaacgg tgttgatcag ctgacgtgcg gaagacatca gtttgctcca gaagtcctgg
    10321 tggtgaccac aaaccaccag atccatatcg tatttcttga ttgcatcgac cagaacctgg
    10381 cctaggtcgc cgctgccgct cagggtttca gtgattggat agcctgcatt agtggaaagc
    10441 tcggtcagcg catgatgtgt ctcttcagag atgcgtttct gcatatcacc cagattcaca
    10501 tcaataagcc cggtgtatag gtcagagtag tttacatcta cgtggatcag agaaactttc
    10561 gcattgtagg ggcgagccat agagactgct ttctctacca gaactttgct ttccggggag
    10621 agatcgactg cgatgagaat gtgtttataa gccatagtgt tactccttcc ataaagttgt
    10681 cgatgactgg ccagctagcg tttcttgtgc cgttacgtca cccgcgtcct gcgaactacg
    10741 tgcgcggggc taacccagcc gtaaagattc agtgttaaga ccatccttac cttatagcga
    10801 cccggatgat ccgtcaatcc gccttgctta cccgttagat aaacaaattt tcgacatgaa
    10861 tattatattg atagtggtta accttctgga aaaaaaacaa cctgatctcc tacactatct
    10921 atagagccgc tcggatgttg gtcaccacaa aagcggtttc tccaggacct ggtgttaact
    10981 gattggcagc gtatcgggag cggtagcccc ggggtggaac tccgcgggca ggtcgccggg
    11041 gaggaggtat gataagcacc gtcgcattat tttgggcttt atgtgtcgtt tgcattgtta
    11101 acatggcgcg ctatttctca tcactacgcg cgttgttagt ggtactgcgt aactgcgatc
    11161 cattgctcta tcaatatgtt gatggagggg gcttttttac ctcacatggc caacccaaca
    11221 aacaggtgcg tctcgtttgg tatatctatg cccaacgtta tcgcgatcat cacgatgatg
    11281 agtttattcg ccgctgtgag cgggtgcgtc ggcagtttat tctgactagc gcattgtgtg
    11341 gtctggtggt ggtcagcctg attgcattga tgatttggca ttaagcactt cagcaataat
    11401 aacgtataaa aaaagcgggc cagtaaactg acccgctcgt tttggtgcgt acgattacag
    11461 gaactgcaag gagaaccagt acagcccgcc ggaaagtagt accgcagccg gaagggtaaa
    11521 cacccaggcc atcagaatac tggttacggt tttacgctgt aagccgccgc catctaccac
    11581 catcgtcccc gcgacagaag aggagagtac gtgagtggtg gaaaccggca tcccggtata
    11641 actcgccagg ccgattgaca ctgccgccgt catctgggca gacatcccct gagcgtaggt
    11701 catgcctttc ttaccgattt tctcaccgat agtcgttgcc acacgacgcc agccaatcat
    11761 cgtaccgata cctaacgcca gcgcgaccgc catgatgatc cacaccggcg catactcgat
    11821 ggtgctaagc atgtcggact tcagtttctt caacaggcgc tgatcgtcag cactcacacc
    11881 aggcatcttc accactttgt cgatagtgtc agaaacgcac agcataatgc ggcgcatctg
    11941 gctacgttga tcaagcgaca gcttgtcgta gctttccaca tcggtggtca acataccttt
    12001 caggcggttg agcgcgttaa tggtattcga cggatggcag tggaactccg caggttgcgt
    12061 tgcgccagct tccggagccg gtactaactg atcagcaccg gtagcctgtt tgagcagcgc
    12121 aggatgctgc tcaaagtaag cttcgacgtt gttgatggca tcacgggtac gggtgatttc
    12181 gtagccagtg gcattcatgt tcaccacgaa gcctgctggc gcgacgccaa tcaataccaa
    12241 cataaccaga ccaatgcctt tctgaccatc gttcgcgccg tgcgaaaacg ccacgccgat
    12301 agcggaaagg atcagcgcga tacgcgtcca gaacggcggc tttttcttgc cgtctttctt
    12361 ttcacgctcc gctggggtca ggtggatacg ggcgcgtttc ttggtgccgc tccagtagcg
    12421 acgcagcaag aaaatcagac cgccagcaaa caccaggccg acaatagggg aaacgatcag
    12481 agaaccgaaa atacttaata ctttcgggat attgagtgca tccaccactg acgtcccggt
    12541 catcaacgca ttggttaaac caatcccgat gatcgcgcca atcagcgtat gagagctgga
    12601 tgcaggtaaa ccaaagtacc aggtacccag gttccagata atcgccgcca gcaacataga
    12661 gaacaccatg gcaaggccat gagacgatcc catattaagc agcagatccg tcggcagcat
    12721 atgcacaatg gcataggcaa cactcagacc acccagcaaa acacccaaaa agttgaacac
    12781 cgccgccata accacggcga gctgagaacg catcgcgcgg gtatagataa cggttgccac
    12841 ggcgttggct gtgtcatgga aaccattgat ggcttcgtag aacagcacaa aagccagtgc
    12901 aagcaataat aacagcccgg tatgcaaatc caggccagca aacaaatgta gcataggacg
    12961 ttacgccatt ttgaggacat gaacgcggcg cattatcagt gactttcgcg gcgcgggcaa
    13021 agtgaaatat agactttttt tgatttgcct cctgtatgga tttcactcaa aaaataatta
    13081 tcttatataa ttcaggcaaa tacttccttt tagtaatatt gatgctggtg cgaccactga
    13141 ggaatcttta caattcacgc ccgttttttc taagaggagc gcaacgtgga aaggtttgat
    13201 gccattatta taggcgctgg tgcggcgggt atgttctgtt ctgcgctggc aggtcaggta
    13261 ggacgccggg ttctgctgat cgataatggt aaaatactag ggcgcaaaat ccttatgtct
    13321 ggcggtgggc gctgcaactt taccaacctt tatgtcgaac caggcgctta tctgagccag
    13381 aatccgcatt tttgtaagtc tgcactcgca cgttttaccc agtgggattt cattgatctg
    13441 gtcaataaac acggtatcgc ctggcacgag aaaacgttag ggcaactctt ctgcgatgac
    13501 tccgcgcagc agattgtcga catgctggtg gatgagtgcg agaagggcaa tgtaaccttc
    13561 agattgcgta gcgaagtgct gagtgtggcg aaggatgaaa caggcttcac gcttgatctg
    13621 aacggcatga ctgtcggttg cgaaaagctg gtcatcgcga ccggtgggct gtcaatgccg
    13681 gggctgggcg catcgccgtt tggttataag attgccgaac aatttggcct caacgtgctg
    13741 ccgacccgcg cgggtctggt gccattcact ctgcataaac cgttgctcga agagttacag
    13801 gtgctggcgg gcgtggcggt gccttccgtg attaccgctg aaaacggcac cgttttccgt
    13861 gagaacttac tcttcaccca ccgcggctta tctggaccgg cggtgttgca gatttcaagc
    13921 tactggcaac cgggtgaatt tgtcagtatc aatctgctac cggatgtgga cctcgaaacc
    13981 ttcctgaatg agcagcgtaa cgcacatccg aatcaaagcc tgaaaaacac actggcggtt
    14041 catctaccga agcggttggt tgaacgctta cagcaactcg ggcaaatccc ggatgtttcg
    14101 ctaaaacagc tcaacgtgtg tgaccaacag gcactgatta gcacattgac cgactggcgc
    14161 gtacaaccca acggcactga aggctatcgc actgccgaag tgacgctcgg cggcgtggac
    14221 accaacgaac tttcttcacg gacgatggaa gcgcgcaaag tgcctgggct gtacttcatc
    14281 ggcgaagtga tggacgttac cggctggctg gggggctata acttccagtg ggcgtggtcg
    14341 agtgcatggg cttgtgcgca ggatttggcg tcaattgtga aataaccatg acgttttccc
    14401 gtcagccgtt tgtatatgtc gttattaagt cattttggta cggttctgca tccgacattc
    14461 tggtgttaac tcgctgtttt gttagcgaat taacgctatt gccgttgtgg ttattttata
    14521 gtgtatttat tctcagagaa tatatattac tgaaataaaa aacacaaaga ataaccaaag
    14581 gaaattattt gaaacgtaaa aggtatatat ttttaatctg taaaatttaa aatcacctcc
    14641 tctggattga atcaacttgc tctatttaag acgattgttt ttacattttc accacctgta
    14701 tcaggtggtg tttttttatt ggcggcaatg cctttatctt atttttatta cgttgtaaat
    14761 atggctattc ctaaatgaaa tttgatttgc tcagtttatt tgtgatgtca ggggagtgct
    14821 tgccatgaga gtcgtgatat tattattcat catttttagg taatgctgcc aacttactga
    14881 tttagtgtat gatggtgatt ttaaggtgct tgcgtggctt
//
LOCUS       DAEEUE010000094        14566 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.094, whole genome shotgun sequence.
ACCESSION   DAEEUE010000094 DAEEUE010000000
VERSION     DAEEUE010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14566
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.094"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..581
                     /locus_tag="KLK84_003142"
     CDS             <1..581
                     /locus_tag="KLK84_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HBE2010378.1"
                     /translation="GALTFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSGPFYCVD
                     EVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELV
                     ERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLA
                     KCLQKGGDCQGYLFRCLHCGKLRLWGDYFVVI"
     gene            complement(635..1357)
                     /locus_tag="KLK84_003143"
     CDS             complement(635..1357)
                     /locus_tag="KLK84_003143"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBE2010379.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(1359..2530)
                     /locus_tag="KLK84_003144"
                     /pseudo
     CDS             complement(1359..2530)
                     /locus_tag="KLK84_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            complement(2557..4173)
                     /gene="bglH"
                     /locus_tag="KLK84_003145"
     CDS             complement(2557..4173)
                     /gene="bglH"
                     /locus_tag="KLK84_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HBE2010380.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMIDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNNIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDAQFAVGAQAEIWW"
     gene            complement(4259..5653)
                     /gene="bglB"
                     /locus_tag="KLK84_003146"
     CDS             complement(4259..5653)
                     /gene="bglB"
                     /locus_tag="KLK84_003146"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HBE2010381.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(5672..7549)
                     /gene="bglF"
                     /locus_tag="KLK84_003147"
     CDS             complement(5672..7549)
                     /gene="bglF"
                     /locus_tag="KLK84_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HBE2010382.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(7683..8504)
                     /gene="bglG"
                     /locus_tag="KLK84_003148"
     CDS             complement(7683..8504)
                     /gene="bglG"
                     /locus_tag="KLK84_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HBE2010383.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(8791..9516)
                     /gene="phoU"
                     /locus_tag="KLK84_003149"
     CDS             complement(8791..9516)
                     /gene="phoU"
                     /locus_tag="KLK84_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBE2010384.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(9531..10304)
                     /gene="pstB"
                     /locus_tag="KLK84_003150"
     CDS             complement(9531..10304)
                     /gene="pstB"
                     /locus_tag="KLK84_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HBE2010385.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSCADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(10396..11286)
                     /gene="pstA"
                     /locus_tag="KLK84_003151"
     CDS             complement(10396..11286)
                     /gene="pstA"
                     /locus_tag="KLK84_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBE2010386.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(11286..12245)
                     /gene="pstC"
                     /locus_tag="KLK84_003152"
     CDS             complement(11286..12245)
                     /gene="pstC"
                     /locus_tag="KLK84_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HBE2010387.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(12332..13372)
                     /gene="pstS"
                     /locus_tag="KLK84_003153"
     CDS             complement(12332..13372)
                     /gene="pstS"
                     /locus_tag="KLK84_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBE2010388.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(13620..>14566)
                     /locus_tag="KLK84_003154"
     CDS             complement(13620..>14566)
                     /locus_tag="KLK84_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE2010389.1"
                     /translation="GYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYY
                     TSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYN
                     CNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADV
                     YIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGI
                     LNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVY
                     GEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         3757 a         3718 c         3317 g         3774 t
ORIGIN      
        1 tcggggcgct tacattcaaa tatcatccca agccactgga aacaggcgca tttgaacagg
       61 ataaaaccgt agagtgcgat tgctgtgaac aacagacgtc agtttattac tcgggtccct
      121 tttattgcgt tgatgaagtt gaacatctct gtccgttgtg tattgcggac ggttctgctg
      181 cagaaaagtt tgcaggtagt tttcaggatg atgccagcat agaaggtgtt gaatttgagt
      241 atgatgaaga ggacgaattt gccggtatta agaacacata tcctgatgaa atgctgaaag
      301 agttggttga acgcacgcca ggttatcatg gatggcaaca ggaattctgg cttgcgcatt
      361 gtggcgattt ctgtgctttt atcggctatg tgggctggaa tgatataaaa gatcgcctcg
      421 atgaatttgc caaccttgaa gaagattgtg agaatttcgg tattagaaat tctgatctag
      481 ctaaatgcct gcaaaagggt ggtgattgtc agggttatct cttccgctgt ctccactgcg
      541 gcaagctgag actgtggggt gattatttcg tagttattta aataatgaga acaggccgga
      601 gcgtaattca tacatccggc cttatttctt cagcttaacc gaacgctaat tctgcggcag
      661 cggctttatc agcgattacc atcagcgaag gatgcagctg caacacggaa gctggaacat
      721 cttcggtaac cggaccttgc agcacatttt tcagtgcctg cgctttacct gcgccactga
      781 caattatcag caagtttttc gccgccatga tgcttttcgg ccccatcgtc acgtagctgt
      841 caggcactaa cgaaaagtcg ccgcccaact caccatgcgc cacaatatca accatctctc
      901 cctggatcgg gaactccacg gtttgctcat ggaaatgggt cgtattcggc aaattgccac
      961 aaaaatgacc atctgcacct aatcccagca ccaccaaatc caggccgcct tcacgcgcca
     1021 gtttctgatc atgctcgcgg tagttatcaa tagtgagctt ctggatattc tcttctttga
     1081 tccccgcagg ggtgaaaaac agattacgca gattggtaat cgttacgcct tctccctctt
     1141 tgccgcgaaa gggaatttca tcgaaattat agaaatagca gttatcgtac caaggcttac
     1201 ctttaaccag ggtggtgagg tattcataca tgcctttggg cgtgctaccg gcggtaattg
     1261 ccaggttaac acgacgcgtc ttcgacatat aaccaagcag atggtgtgcc gcgacacggc
     1321 tcatttcctg gtaatcttcg gtaatgatta atttcatctt aatgccttat tttaaatttc
     1381 tgtacgaagg ctgcataggc aggccgccag acatccattt catggttcag gccgggatat
     1441 tcctggtaat caaacttaat ttttttctgc tcaagctcag ttttcagccc ggcgatatcc
     1501 ttgccggtta cgacatcttt atcaccaacg accacagtaa aattacgtag ttgctggtta
     1561 atagctgccg gatcgttcag ccgggccgcg acaccttcat ccggtacggt tgtcgtggta
     1621 acaccactga atgtggccag ccaaagcttt ccagatgatt cattccggaa accagcgcct
     1681 ggtacccgcc ttgtgaaagc cctgccagcg cgcgaccatc ggcatcttta cggacactaa
     1741 aacgcttgct aatcagcggg ataatatcgt tcatcagttc gcgatctgcc gctttagcat
     1801 tcagcggata aaagacttta cgtctttcct gaggcacgaa atcttcggga ataatgccct
     1861 tcgcatcggt ttcggtatcc gggatcacca ccagcatcgg tttaattttc ccttcagcca
     1921 gcaggttatc catgatttgc gggatacgcc cctgatcgat agcggaacgt ccggtatcac
     1981 caaaaccgtg atagaaatag agcactggca aaggctcgcc catgccggtg tatcctggcg
     2041 gggtccagac atacatctga cgttcagatt gcaatgcgtt ggagtggtaa gttatggcga
     2101 tcaaatcacc atgcgcaaca gaacgcgtat ccagataact gcctggcacc agaatcatac
     2161 tggagttaac ctggcgctga ggcttagtca ttgcggtgcc tgtatcaatg ctgcgtacac
     2221 catcaacatt gaaaaaatac tcgtacaggt tgcctttcag gataggtgtg cgccacgacc
     2281 agactcctgc ttcgtctttg gtcatcgggt gaatattgtc cggaaccgga acacccacca
     2341 ctacagagac attttttgcc ccaggggcaa agtagcgaaa ggtcacgctg ttatcggcat
     2401 tgacctgagt cacatactgt ttcaccggga tagtgggtgc cgggctggct ggcatatccg
     2461 ctgctatggc ccactgagca gaaataccgc tggcaatagc cagcgttaaa gcagcaattt
     2521 ttatattcat cgtaatattc ctttcattaa gttccattac caccagattt cagcctgggc
     2581 accgacagca aactgggcgt ctttattgtc ttcgaaggtg aagccatcca gttcgttttc
     2641 cagggctttg atatacgtgg cgtagaaacg aatttccaga cgcgaggtca acatactggt
     2701 attgacttta aaggtatgga agagcgtggt tttataacca gactcattaa atttattact
     2761 attcgcatct ttgttttgct gggtgaaata gcccagttca acacctgtct ggttatattg
     2821 gtcccaaata taggccgggc gaacaaccgc acgaatagat tcgaaatcag agtgtgcgcc
     2881 tgtttcgtag ctataaatat tgttaccgaa ggagtaaaca atcgcgttag ccacaataaa
     2941 atgatcgccg atataggctt cgccctgcga ggtcagacga actgctgttc cgccggtgtg
     3001 atcaccataa taacgaccat taaaggtggt aaatggactt gcgccagcat aacggccaaa
     3061 gttcctggcg atagagttat tcgcaaccag gaaggagaat tcgttgaagc cacctttatc
     3121 aaatttctgc gttaaagatg tgccgaacat ccaggtatct ttccagtcat aatacccgtt
     3181 attatcctga ttatcttttt ccgatgcgct ttcgtttgcc gtgacataac gaccactcac
     3241 cattaaggtc gctttatccc ataacgggat atctttatag cgtaaatcaa tagtatgggt
     3301 attaatctgc tgtttgtttt gcaggctgcg atcgtaatca tcgacatctt cgcgaaccag
     3361 cgcgatatca attttacccg gaccgacttt ccagttttcc agacctacac ccgctgcagc
     3421 atcagtacgc tgcgttttcc agtccagcat ctggatttca attttcggcg caccgtgttt
     3481 acccacccag aaatcagcct ctggcgcaaa gggcaggaaa cctttggtgg taacgtacat
     3541 atcggagaac tgcataaagt tctcgccacc ggcattatcg ccaaaccagc cggtagagta
     3601 ctgctgacca acgttaccat ctatcatcac aacggcatca acccgtttgc cgttttcgtt
     3661 gtagacacgt tgttttaact gcaaatcaaa ccagccggag tattcgttac caaagcggcc
     3721 cagagaacca atcgcccatg atttaggtga accatgagag gcggtccccc aaccagaacg
     3781 gtaatagccg ttgtaactaa agccaatttc atctttcaca aacttactga aatctttcag
     3841 agtcatggaa gaataaatcg agcctgtctc actggcattt ttctcttcat ttttgaccag
     3901 tacagcatca ggttttgcgg cactactggt cgggaacgga ttggcggcat acccttgatc
     3961 attcgtactt acgctacgat tcaccgtcgc tggcgtatat tttttcttct cttcatcttt
     4021 atactttttg agttcgcttt gcgtttctct taacgctttt tctaataact caaggcgttg
     4081 ttccaccgtt aatgattcag ccaatgattg tgcagaaaag gctaacggtg ccattaataa
     4141 gatggcagag gtaataagat ttcgtctaaa cataatatcc ctttatggtg caaagaaaga
     4201 attaacgtat cgcatccaga ctgttctgaa tgcgacgata attaaggtgc tttattggtt
     4261 atttttttaa tgacagcccc cgcgttttga tcacttctgc ataccatccg aagcttttct
     4321 tacgcgttct tgtgaggctt ccttcgccat tatcatcacg atccacataa ataaagccgt
     4381 agcgcttaga catttgtgaa tgagatgcac tgactaaatc aattggcccc caactggtgt
     4441 accccataat atccacacca tcggcaatcg cttcatttac ctgtaccagg tgatcgttta
     4501 aataggcaat tcgataatcg tcctgtatcg aaccatccgc ttcaacgctg tcttttacgc
     4561 ctaatccatt ctcgacaata aataacggtt tttgataacg atcccaaagc gtatttaaca
     4621 gaacccgtaa tccaaccgga tcaatttgcc acccccactc tgaacttttc agatgcggat
     4681 tggggatcat attcagtatg ttgccctgcg catttttatt aatgctttcg tcgtgggaaa
     4741 cacaaccagt catgtaataa ctaaaagaga tgaaatctac agtatgtttt aaatcttctg
     4801 cgtcactttc agtcatctca atggtgatat tgtggtcgcg gaagaaacgc tgcatatagc
     4861 cgggatattg gccacgcgcc tgaacatcac caaagaacat ccagcgccgg ttctcttcca
     4921 tggcctgcaa catatcctgt ggctggcagg tgagggggta aaccagccca ccgagaagca
     4981 tattgccgat tttcgcttcg gggatcaggc tatgataggc tttaactgcc cgcgcactgg
     5041 caaccagttg atgatggatc gcctgataaa cttccgcctc gccactctct tctgccagcc
     5101 ccacgcccgt gaatggcgcg tgtaacgaca tattgatttc attaaacgtc agccataacg
     5161 ctactttatg ttggtagcgg gtaaagaccg tgcgggcgta atgctcgaag tgatcgatga
     5221 ccgcccgatt agcccaaccg ccgtagtttt tcaccagccc atatagcatt tcgtaatggg
     5281 ataacgttac cagcggcttg atccccgcct gcgccatttc atcaaacagc cgatcgtaaa
     5341 acgctaaccc cgcttcattc ggttcggctt cgtcgccctg agggaaaatt cgcgcccagg
     5401 caatggaaat acgcagacag gtgaagccca tctcggcaaa taacgcgata tcttccgggt
     5461 aacggtaata aaaatcgatg gcgacatctt tgatattctc tttccccagg atgcgcggtt
     5521 ccatttttcc cattacgcca tgaggctgta aatctgaggt cgtgatccct ttgccatctt
     5581 ccagccaggc accttccacc tgattggcag ctgttgcacc accccaaaga aatgtttctg
     5641 gaaatgcttt cataattaac tccttttatc gttagcgaat gatggataac agcggttcac
     5701 ctgcgcttat ctgcgccgtg ccgtggggta atacgtctgt aaaatcatcg ctattactga
     5761 ttaataccgg cgtcgtcaga tcaaatccgg cctcgcgaat agcagggata tcaaaagaaa
     5821 tcagccgatc gcctgtattg accttgtcac ccacgttgac gtgagcggaa aagaatttgc
     5881 cgtccagttt tacggtgtcg ataccgacat gaatcaggat ctccacacca tcatctgact
     5941 caatgccaat ggcgtgtaat gtggcgaaca acgaagcaat tcgacccgca accggagaac
     6001 gcacttcacc aaccgagggc agaatggcga tacctttacc caacaggcca ctggcaaacg
     6061 tggtatcagc gacgtgaatg agcggcacaa tctctcccgt catcggtgaa cagataccgc
     6121 cctgctcagg tggtgtaata acctctggtg ttttctcttg cggggcaccc tgcgctggct
     6181 gacgtttact ggtgatgaaa tgaagcatca acgtaccaac aaatgcgcaa cctatggcaa
     6241 tgacaccgcc aataacgctg gcccagacgg tgaaatcaat ccccgttgac gggatggtct
     6301 gcatgaaggt gaaaatactt ggcaaaccaa aggagtaaac tttcgtttgc gcgtagccaa
     6361 tgatggtggc ccccaatgct ccactgatac aggcgataac aaaggggtac ttacgcggca
     6421 ggttgacgcc atataccgct ggttcggtga taccaaacag acccgtcaac gccgctgatc
     6481 ccgccaccac ttttttctgc gcatcgcgtt cgcagaggaa gacgccgagc gccgccccga
     6541 cctgcgccat aatggcgggc attaacagcg ggatcatggt gtcgtagccc agcacggtga
     6601 agttattgat acacagcggc accaggcccc tgtgcagtcc gaacatgacg aagatttgcc
     6661 agaagccgcc cattaccgcg cccgcaaatg caggaaccgc ctgataaagc cagagataac
     6721 cggcggcaat catttcgctt atccaggttg atagcggccc caccagcaga aaggtgacgg
     6781 gtgtgataac catcagacat agcaatggtg tgaagaaatt tttgattgcc gacggtaacc
     6841 acgcattaag tcggcgttcc agaatgctgc acaaccaggc agaaaaaata atgggaataa
     6901 ccgatgacga gtaattcaac aatgtgaccg gaatacccag gaaatccagc cccggcgcat
     6961 ccgctttttg cccgttctcg aaggcagtca gaattaatgg atgcactaac gctccaccaa
     7021 tcaccatggc agtaaatgga ttaccgccga agcgtttgcc cgcggtgtat cccaggatta
     7081 tcgggaagaa cccaaacaag gcatcactgg cgctgaataa aattaaataa gtaccacttt
     7141 gttcggtcgt ccactgaaaa gtgagcgcca gagccagcat acctttcagg atcccggttg
     7201 ccgccatcag accgatcaga ggcgtaaaaa tacctgaaat aacataaaca aagcggttta
     7261 gcagattacc tttctcatca ttttccggtg cctgttgcgc tttttcgcca aggcctgcca
     7321 cgctgttaac cgccaggaag acatcggcca catggttacc tatgaccacc tgaaactgac
     7381 caccgctttc caccaccata ataataccgg gggtcttttt cagtacctca gcttgcgctt
     7441 tgctttcatc ctttaactta aaacgtaatc gcgttgcgca atgcatcaga ctcacaatgt
     7501 tatctgcgcc cccgactcct gcgactattt ttctggctaa ctccgtcata acttgccctc
     7561 taccgctttg cggcaaaact ccaaaaaaaa acctgaaaaa aacggcctga cgtgaatcaa
     7621 gcaatttttt tcaggttttg cccgcttagt gcggtaacaa tcctttactc agtaataata
     7681 tttcagtgtt ctttgcgcac gcgctctata tttatggcta aaaacataat ctctgcgggt
     7741 gaaattttac gttgatactg caaaccaata aaaatggcga tccgttccgc acattgccat
     7801 gcttgcgggt aattttgttt tactgcttgt tgtaatgatt catcactatc gttaattgaa
     7861 gcatgttcaa gaatacgcca agataaaaac ttcagatgtg taaccagtcg ctgataactc
     7921 aagctttctt cctggtaatt aaggctgaac tgaaatttta ttaattgcag catttcgcgc
     7981 attaactgcg tgacacctgc aacatcctcc atatttccgc tcatttgggc actgaccaga
     8041 tgcatggcaa taaagcccac ttcatctttc ggtaactgca cgcccaaccg tttatcaata
     8101 atggttaacg cttcttcccc tagctggaac tctttcgggt aaagccgctg gatatcccac
     8161 agcaacacgt tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt tagcgagata
     8221 taaatactgt cctgtaattt tcccaagcgc tcctgcgcta aagagataag acgatcacag
     8281 gttgccatca cctcaagagg aatatgactt aagagttcgc ttaatcgccc gttcagttca
     8341 tgactgctca aggcatactc tttttctatt ccacttgagt taattctttc gccagcgcgt
     8401 ttttgaaagc caattccgcg ccccatgacg actttttccc gctgttgatc atcaataacc
     8461 accacaacat tattgttgag aattttggtg atttgcatgt tcatagcaag gacctttttt
     8521 ataaacaaaa aaaaaccgac ttcaccagta ttctctggtt atgtcaggtt ttgcctgcga
     8581 atgcagtaac aatccagtca tttattaatg gtttttataa cgaacatcca ggttcggaaa
     8641 ttaatttaat tgcgtgcaga gaaaatagca atgcgctatt gataaaaata tgaccatgct
     8701 cgcagttatt aactttatgt aattttagga atttataaag ttatatataa caaatcccaa
     8761 taattaagtt attgggattt gtctggtgaa ttatttgtcg ctatcttttc ccgccagcag
     8821 tttatccagc tcatcaccgc ctacatgacg gaaatcctga cccttcacgt agtagaagat
     8881 aaactcacaa atattctggc agcggtcgcc gatacgttcg attgaacgcg cacagaacag
     8941 cgcggtaagt acgctcggaa tagtgcgcga atcttccatc atgtaggtca tcagttgacg
     9001 aacaatacct tcgtattcct gatcgacttt tttatcttca cgataaatac gtaccgcttc
     9061 gtcaatgtcc atccgcgcga acgcgtccag cacgtcgtgc agcatctgga tggtatgacg
     9121 gcccagcgac tccagactta ccagcaacgg ctgatgctgc tgggagaatt tctccagcgc
     9181 agtacggcag attttgtccg ccacgtcgcc aatacgctcc agctcggcaa tggttttact
     9241 gatcaccata accagtcgca ggtcgctcgc cgtcggctga cgtttggcga taatgcgcac
     9301 gcaggcttca tcgatcgcca cttccatcat gttgacgttc ttgtcgcctt cgatcacgcg
     9361 cttcgccaga tcgctgtcct ggttatgcat cgcggtgatt gcatcagaaa gctgctgctc
     9421 caccatgccg cccatggtca tcacctgcgt gcggatactt tccagttcgg cgttgaactg
     9481 gccggaaata tgtttattaa gattgagact gtccataacg cactcctgaa tcaaccgtaa
     9541 cgaccggtga tgtagtcttc tgtttgtttc ttcgctggct tggtgaacag atcgtccgtg
     9601 ttgctgaact caatcaattc gcccaggtac ataaatgccg tgtggtcgga acaacgcgca
     9661 gcctgctgca tgttgtgggt gacgatcacc acggtgtaat cctgcttcag ttcggtgatc
     9721 agctcttcaa tacgcccggt agagatgggg tcgagcgccg aacacggttc gtcgagcagc
     9781 agcacttccg ggcgaatggc gataccacgc gcaatacaca gacgctgttg ctgaccacca
     9841 gagagagagt aaccgctctg gtgcaattta tctttggttt cgttccacaa tgcggctttg
     9901 gtcaatgccc actgcacgcg ctcgtccatg tcggcacagg agagcttctc aaacagacga
     9961 acgccaaagg cgatgttgtc gtagatggac atcgggaacg gcgtcggttt ctggaacacc
    10021 atgcccactt tcgcacgcag cagggcgata tcctgagagt tggtcaggat gttgtcgcca
    10081 tcaagcagaa tttcaccttc cgcacgctgc tccgggtaca gttcaaacat tttgttgaag
    10141 gtacgcagca gcgtcgattt accgcagccg gacggcccga taaacgccgt aacctggttt
    10201 ttggcgatat ccaggttgat gtttttcagg gcatggaatt tgccgtagta gaagttcaaa
    10261 ttacgaacct gaattttact cggggcagtt tcaaccatac tcattgcaat cttttcctca
    10321 tcgtacctga tgcttttcgc taatcaggct taccgtaggg ccgggtaagg cgttcacgcc
    10381 gcatccggca aagtgttaac cgtgtttatt cttcgcaaaa acaacgcgcg ccagaatgtt
    10441 cagcagcagg acgcacaggg taatgatcaa taccccggcc caggccagtt gttgccattc
    10501 cgcaaacggg ctcatcgcaa acttaaagat cgtcaccggc aggttggcga tcggctgcat
    10561 catgtccgtg ctccagaact ggttggagag cgcggtaaac agcagcggcg cggtttcacc
    10621 ggcaatacgg gcaatcgcca gcaggatacc ggtcataatc ccggacaccg acgctttcag
    10681 cgtaatcgcg gagatcatct tccacttcgg tgtacccagc gcataagccg cttcacgcag
    10741 gctgtccggc accagtttca gcatgttctc agtggtgcgg ataacgatcg gcacctgcaa
    10801 cagcgccagc gcaattacgc ccgcccagcc ggagaagtgc tccatctgtg ccaccacgat
    10861 ggtgtagaca aacagaccaa ccacaatcga cggcgcggag agcagaatgt cgttaatgaa
    10921 gcgaatcact tctgccagcc aggatttacg accatattcc gccagataaa tccccgccat
    10981 aatgcccagc ggcgtaccga ataccgtggc ccacaaaatt aacagcccgc tacccgccag
    11041 ggcgttcgcc agaccaccac cttccgtatt gggcggcggc gtcatttcag tgaacagcgc
    11101 cagcgacata ccgtcgatac cgcgagtgat ggtggacatt aaaatccaga tcagccagaa
    11161 caggccgaag gccatcgtcg ccatcgagag cgtcagcgca atacggtttt tgaggcggcg
    11221 acgcgcctgc attttgcggc gagattcagc cagcgccgca gtggtttgca tttcaaccat
    11281 agccattagc gtgccccctc attcttagcc aggcgcataa tcataaactt cgatgcggcg
    11341 aggacgatga aggtaatcac aaacaggatc aggcccagtt ccatcagtgc ggcaacgtgc
    11401 agaccggatt ccgcttccgc aaattcgttc gccagcgcag aggtgatact attgcccggc
    11461 atgtacagcg aggcgctgtc gagctggtag gtgttaccga tgataaaggt caccgccatg
    11521 gtttcaccga gcgcgcggcc cagccccagc atgatgccgc cgataacacc atttttggtg
    11581 aacggaagaa cgatacgcca gataacttcc caggtggtgc agccaatacc gtaggccgac
    11641 tctttcatca tcaccggggt ttgttcgaac acatcacgca ttaccgccgc aatgtacgga
    11701 ataatcatga tggcgaggat cacgcctgcc gcgaggatac cgataccaaa tgcggggcca
    11761 gagaacagcg cgccaacaat cgggatattc gacatgatat tgccgaccgg ctcctgaaag
    11821 taaacggcga acagcggcgc aaagataaac aggccccaca tgccgtaaac gatacttgga
    11881 atggctgcca gcagctcaat ggcgataccc agcgggcgtt tcagccagcc aggcgcaagc
    11941 tcagtcagga acagggcgat accgaaactc accgggacgg cgatcagcag cgcgataaac
    12001 gaagtcacca acgtaccgta gatcggcacc agcgccccgt agatatcgtt cggtgcatcc
    12061 cactctttgg tccataggaa agccagacca aatttctgaa tgctcggcca ggaggagatg
    12121 atcagagaga caataatgcc acccaacatc aatagcacaa tcagcgccgc cagttttacc
    12181 agcacgctga aaattatgtc gcccttttta cccggtgggt taaaagcggg cttggttgca
    12241 gccataagtt actcttcagt taaacgcgtt tacaaagtca ttttgtaatg ccggatgcgg
    12301 cgtaaaacac cgtacccggc ctggagtttt attagtacag cggcttaccg ctactgtctt
    12361 taatattggt cttccacgca gcgcgaacct gttcaactac actatccggc aggctggcgt
    12421 aatccaggtc gttcgcctgt ttagccccgg ttttgtacgc ccagtcgaag aatttcagca
    12481 cctctgtgcc ttgttctggt ttcttctgat ctttgtggat cagaatgaac gtggtagagg
    12541 taataggcca tgcatcttcg cctttctggt tggtcagatc ctgagcgaag gttttgctcc
    12601 agtctgcacc ttttgctgca ttagcgaagt tttcttcggt cggactaacc ggtttaccat
    12661 cagcggagat cagtttggtg tacgccaggt tgttctgctt cgcgtaagca tattcaacat
    12721 aaccaattgc acccggcaga cgctgaacga acgcggcgat accgtcgtta cctttaccgc
    12781 ccagaccgat cggccatttt acggtagagc cagtaccaac gttgtttttc cactcttcgt
    12841 tcactttcgc caggtagctg gtgaagacga aggaagtccc ggagccatct gcgcggcgta
    12901 ctacagcaat gttttgtgaa ggcagtttca gacccggatt cagtttggcg atggcttcat
    12961 catcccactt cttgattttg cccaggtaga tgtcgccgag ggttttacca tccagcacca
    13021 gttcgccaga cttcagccct ggaatgttaa ccgccagcac cacgccgcca atcacggtcg
    13081 ggaactggaa cagaccttcc tgagccagtt tttcgtcaga cagcggcgcg tcagaggcac
    13141 caaaatcaac ggtattagcg ataatctgtt ttacgccacc ggaagaaccg ataccctggt
    13201 agttaacttt attaccggtt tctttctggt aagtgtcagc ccatttggca tacaccggcg
    13261 cagggaaggt tgcacctgca cctgtcaggc ttgcttctgc aaacacagag aaagcactca
    13321 tcgataaggt cgcggcgaca acagttgcga cggtggtacg cataactttc ataatgtctc
    13381 ctgggaggat tcataaagca ttgtttgttg gctacgagaa gcaaaatagg acaaacaggt
    13441 gacagttata tataaggaat atgacatttt tatgacagaa aggaattacc aaataactta
    13501 ctgattttaa atgtgaagat gaaaatccca catgccatct ttttgtcatg atgcatttaa
    13561 aagtgataaa gaaatgggtg agatgacaac gatgtgatga acaatcgttg tcagacctat
    13621 tagcgcacaa tctcaacagt tatcgtcgct gttgcagtaa attgccccgg ggctgggcga
    13681 gctccagtaa ggctcgcagg agcagctgaa aaagttacac tacctgattt ttcaccatac
    13741 actgttgatt tatccatgtc tgttggcaac tcaccaccgt tcactttgat ttccttatta
    13801 tttttgtcgt aaatttttat ccccacgtta gagtttgaag ttacgaccat tgaattgtcc
    13861 gtattgggtt cggtagcata aaaagacgta ctgatatcat cggtattcag tatccccagg
    13921 ttttcacact caatagtgac tgttttagta attttatgtg ttgttaccgc acttccaaca
    13981 acagatgaaa aatctgcagc tggtatttct ttaaaatcaa aacaaactgg ctttaaatta
    14041 tttatttcgc aactttgcgg agctgtaata tcgccccgaa tataaacatc agccatcggc
    14101 tgtgagtaat ccaccggggt gtcacggcta attgttccat acaatgtcac tatctttgtt
    14161 tcaggaataa tcaattttcc tgcaaatgtc ttcttgatat aaaaatatac tatcgcttta
    14221 gccccactgg atatacttaa aggttcaatg cggttgcaat tgtactttgt attaccagac
    14281 gggttgttag catgatattc aaaaggtgca ttcacatacc caaccccaag aatatctata
    14341 gcaatcccaa catcaaggtt cggattaaga tcataataat taatattaga gtgaacccca
    14401 gcataagtta atgcctggtt tatttttgac gtataataga tataggggaa ttctcctacg
    14461 ttaccagtat cacaatgaca gcgtccaaga tatgaggatc cattagttac agtggtgtta
    14521 aatgtcgttc caaccttatt ttcgttcgcg gtaataagat atcccg
//
LOCUS       DAEEUE010000095        14515 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.095, whole genome shotgun sequence.
ACCESSION   DAEEUE010000095 DAEEUE010000000
VERSION     DAEEUE010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14515
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.095"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(113..379)
                     /locus_tag="KLK84_003155"
     CDS             complement(113..379)
                     /locus_tag="KLK84_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HBE2010390.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            653..4177
                     /gene="nifJ"
                     /locus_tag="KLK84_003156"
     CDS             653..4177
                     /gene="nifJ"
                     /locus_tag="KLK84_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBE2010391.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4544..5673
                     /gene="ompN"
                     /locus_tag="KLK84_003157"
                     /pseudo
     CDS             4544..5673
                     /gene="ompN"
                     /locus_tag="KLK84_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5814..6248
                     /gene="uspF"
                     /locus_tag="KLK84_003158"
     CDS             5814..6248
                     /gene="uspF"
                     /locus_tag="KLK84_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HBE2010392.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6424..7359
                     /gene="ttcA"
                     /locus_tag="KLK84_003159"
     CDS             6424..7359
                     /gene="ttcA"
                     /locus_tag="KLK84_003159"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBE2010393.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="KLK84_003160"
     CDS             complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="KLK84_003160"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HBE2010394.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="KLK84_003161"
     CDS             complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="KLK84_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HBE2010395.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10571..11803
                     /gene="dgcM"
                     /locus_tag="KLK84_003162"
                     /pseudo
     CDS             10571..11803
                     /gene="dgcM"
                     /locus_tag="KLK84_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="KLK84_003163"
     CDS             complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="KLK84_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HBE2010396.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(12641..14095)
                     /locus_tag="KLK84_003164"
     CDS             complement(12641..14095)
                     /locus_tag="KLK84_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HBE2010397.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14092..>14515)
                     /locus_tag="KLK84_003165"
     CDS             complement(14092..>14515)
                     /locus_tag="KLK84_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBE2010398.1"
                     /translation="FSICSRQAAVVRAPIDGIVANRSAHTGSWVEGGTSLVSLVPVSE
                     LWVDANYKENQIAGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATG
                     NFTKIVQRVPVRIAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3873 a         3663 c         3429 g         3550 t
ORIGIN      
        1 acaccattag cgaaatgctg aaagaaggtg cacaagtgga tctgaccgcg aaccagttaa
       61 cgctggcgac cgcgaaacag acattaactt ataagctggc ggatttaatg aattaatagc
      121 tgccacagct cccggcggca agtgactgtt cgctacagcg tttgccgttg ggtaatgcac
      181 acatcccaat cgccgtacca tccagttgac gggcaacaga aagcgaaccg ccgatcattg
      241 cacaatttgc ttctccacta ctggacatcg acgcttttaa acttggcgct acgtgcgccg
      301 cagtcgcctg ctgaacaggc tcactactac acgccgacaa caataaagcg gcacacccta
      361 cccaaaacgc tgctcgcatc tcttcttcct ctgatcttca agccaaacga caccgccata
      421 aataataggc agcacagagg gcggcgtcga gagctgtcct gcgcgttgcc ccgcattttt
      481 acttttttat ggctattttt ttgccctctg tttgatcaaa acattcatta ctctgatgtg
      541 ggggacacaa aagcgaaaat gcagaagaaa gccatttgct aaaattgaag gattactact
      601 gggcgcgcag caatttcgtg cgcccctcat ttgcgcaatg taagggtgtc atatgattac
      661 tattgacggt aatggcgcgg ttgcttcggt gacgtttcgc atcagtgaag ttatcgccat
      721 ctaccctatt acccccagtt ccacgatggc agaacaggct gatgcctggg ccggaaacgg
      781 cttgaagaac gtttggggag acacaccacg cgtggttgaa atgcagtcgg aagcgggtgc
      841 tatcgctacc gtgcatggcg ctttgcagac gggtgccctt tcaacatcgt ttacgtcatc
      901 gcagggtttg ctgctgatga tcccgacgct gtacaaactg gcaggcgaac tgacgccatt
      961 tgtcctgcat gtagcggcac gtaccgttgc cacacatgca ctttctattt ttggcgatca
     1021 ttccgacgtt atggcggtgc gccagacggg ttgcgcgatg ttgtgtgcgg ctaacgtcca
     1081 ggaagcgcaa gactttgctc tcatttcgca catcgcaacg ctgaagagcc gcgtgccatt
     1141 tattcatttc tttgatggtt ttcgcacgtc ccacgaaatc aataaaattg tcccgctggc
     1201 cgatgacact attcttgatc tcatgccgca ggccgaaatt gatgctcatc gcgcccgggc
     1261 gctcaacccg gaacatccgg tgatccgcgg tacgtccgcc aatcctgaca cttatttcca
     1321 gtctcgcgaa gccaccaatc cctggtacaa cgcggtctat gaccatgttg aacaggcgat
     1381 gaatgatttc gctgccgcga caggtcgtca gtatcagccg tttgagtatt acgggcatcc
     1441 gcaagcggaa cgggtgatta tcctgatggg ctctgccatt ggcacctgtg aagaagtggt
     1501 tgatgaattg ctaacccgtg gcgaaaaagt tggcgtgctg aaagttcgtc tgtaccgtcc
     1561 cttctccgcc aaacacttgc tgcaagctct gccgggatcc gtacgcagcg tggcggtact
     1621 ggacagaacc aaagaacccg gtgcccaggc agaaccgctc tatctggatg tgatgaccgc
     1681 actggcagaa gcctttaata atggtgagcg cgaaaccctg ccccgtgtca ttggtgggcg
     1741 ctatggtctt tcatccaaag aatttggccc ggactgtgta ctggcggtat ttgccgagct
     1801 caacgcggct aaacctaaag cacgctttac ggttggtatt tacgatgatg tgaccaatct
     1861 gtcactgccg ttgccggaaa acactctgcc aaactcggcg aaacttgaag ccttgtttta
     1921 tggccttggt agtgatggca gcgtttccgc aaccaaaaac aatatcaaga ttatcggtaa
     1981 ttccacgccg tggtacgcac agggctattt tgtttacgac tccaaaaaag cgggcggcct
     2041 gacggtttct caccttcgcg tgagcgaaca gccgattcgt tccgcttatc tcatttccca
     2101 ggctgatttt gttggctgcc accagttgca gtttatcgat aaatatcaga tggctgagcg
     2161 tttaaaacct agcggcattt tcctgcttaa tacgccgtac agcgcagatg aagtgtggtc
     2221 gcgcttgccg caggaagttc aggccgtgtt aaaccagaaa aaagcgcgct tctatgtgat
     2281 taacgcggcg aaaatcgccc gcgaatgtgg cctggcggcc cgtattaata ccgtcatgca
     2341 gatggctttt ttccatctga cgcaaattct gcctggcgat agcgccctcg cagaattgca
     2401 gggtgcgatt gccaaaagtt acagtagcaa aggtcaggat ctggtggagc gcaactggca
     2461 ggctctggcg ctggcgcgtg aatccgtaga agaagttccg ttgcaaccgg taaatccgca
     2521 cagcgccaat cgaccgccag tggtttccga tgccgcccct gatttcgtga aaaccgtaac
     2581 cgcggcgatg ctcgccgggc ttggtgatgc cctccccgtt tcggcgctgc cgccagacgg
     2641 cacctggccg atgggcacca ctcgctggga aaaacgcaat atcgccgaag agatccccat
     2701 ctggaaagag gaactctgta cccaatgtaa ccactgcgtc gccgcttgcc cacactcagc
     2761 tattcgcgca aaagtggtac cgcctgaagc gatggaaaac gcccctgcca gcctgcattc
     2821 gctagatgtg aaatcgcgtg atatgcgcgg gcagaaatat gtcttgcagg tggcaccgga
     2881 agattgcacc ggttgtaacc tgtgcgtcga agtttgcccg gcgaaagacc gtcagaatcc
     2941 agagattaaa gccatcaata tgatgtctcg ccaggaacat gtcgaagaag agaaaatcaa
     3001 ttacgatttc ttcctcaacc tgccagaaat cgaccgtagc aaactggaac gtattgatat
     3061 tcgtacatcg cagctgatta caccgctgtt tgaatattca ggtgcttgcc ccggttgtgg
     3121 cgagacgccg tatattaaat tgctgactca gctctatggt gaccggatgt tgatcgctaa
     3181 cgccaccggc tgttcttcaa tttatggcgg taacctgccc tctacaccgt ataccaccga
     3241 tgccaacggt cgtgggccgg catgggcgaa ctctctattt gaagataatg ccgaatttgg
     3301 ccttggtttc cgcctgacgg tcgatcaaca ccgtgtccgc gtgctgcgtc tgctggatca
     3361 atttgccgat aaaatcccgg cggaattact gacggcgttg aaatcagacg ctacgccaga
     3421 ggttcgtcga gaacaggtcg cagctttacg ccagcaactc aacgatgttg ccgaagcaca
     3481 tgaactgctg cgtgacgcag atgcactggt ggaaaaatca atctggctga ttggtggcga
     3541 cggctgggct tacgatatcg gctttggcgg tctggatcat gtattgagtt tgacggaaaa
     3601 cgtcaacatt ctggtgctgg atacgcaatg ctattccaac accggtggtc aggcgtcgaa
     3661 agcgacaccg ctgggtgcag taactaagtt cggcgagcac ggcaaacgta aagcgcgtaa
     3721 agatcttggc gtcagtatga tgatgtacgg tcatgtttat gtggcgcaga tttctctcgg
     3781 cgcgcagctg aaccagacgg tgaaagcgat tcaggaagcg gaagcgtatc cgggaccatc
     3841 gctgatcatt gcttatagcc cgtgtgaaga gcatggttac gatctggctc tcagccacga
     3901 ccagatgcgc caactcactg ctaccggctt ctggccgcta tatcgctttg atccgcgtcg
     3961 tgccgatgaa ggcaaactgc cgctggcgct ggattcacgc ccgccgtcag aagcactgga
     4021 agaaacgtta cttcacgagc agcgtttccg tcgtctgaat tcgcagcagc cagaagtggc
     4081 agaacagtta tggaaagatg ctgcagctaa tttgcaaaaa cgctatgact tcctagcaca
     4141 aatggccgga aaagcggaaa aaagcaacac cgattaaatg ctctggataa ggattatcca
     4201 attctaaaaa aaaagcccgg acgactgttc gggcttgtct ttttatatgt tggaaaatca
     4261 gtggcaatgc aatggcccaa cagaaaataa aatctgtgac aataaaggca tataacccgc
     4321 gcagaataac gtataggaaa ttaaaagtat aatttttatt ttgtatgtat tatcttcgat
     4381 acattcacca gacttattat catttcagag gaattatttg attaaggttt tacttaaggc
     4441 gtaacaaatg atatttatcg gctaactgaa cttctccttt aggatgtttt cactccccga
     4501 aatggggata aaaggcaaat aaaataacta aaggatttat tcaatgaaaa gaaaagtact
     4561 ggcactttta attcctgccc tgctcgccgc aggtgctgca catgcagccg aagtttataa
     4621 taaagacggc aacaaattag atctgtatgg caaagttgat ggcctgcatt atttttctga
     4681 taattcagcg aaagatggcg accagagcta tgcgcgtctg ggttttaaag gcgaaaccca
     4741 aattaacgat caacttactg gctacggtca atgggaatac aatattcagg caaacaacac
     4801 tgaatcttca aaaaaccagt catggacccg tctggcattt gccggactga aatttgcaga
     4861 ttacggttct ttcgattacg gacgtaatta tggcgtaatg tacgacatcg aaggctggac
     4921 cgatatgctg cctgaatttg gcggtgactc ttataccaat gcagacaact ttatgactgg
     4981 tcgagccaat ggcgtcgcga cttatcgtaa tactgatttc ttcggtctgg taaatggtct
     5041 gaacttcgcg gtgcagtatc aaggtaacaa cgaaggagcc agtaatggtc aggaaggcac
     5101 caataacgaa cgcgatgttc gtcatgaaaa cggtgacggc tggggtcttt ccacaacata
     5161 tgatttaggc atgggcttta gcgctggtgc ggcatacacc tcttctgacc gcaccaatga
     5221 ccaggttaac catactgcgg cgggtggtga taaagcagac gcgtggactg ctgggctaaa
     5281 atacgatgct aacaatattt acctagcaac catgtattca gaaacgcgta atatgacccc
     5341 gtttggcgac agcgattatg cagttgcgaa caaaacccag aactttgaag ttactagtac
     5401 cagtttgatt ttggtctgcg tccggcagtc tctttcctga tgtctaaagg ccgtgacctt
     5461 cacgctgctg gtggtgcaga caacccggca ggtgttgatg ataaagatct ggttaaatac
     5521 gccgatgttg gcgcgactta ctatttcaat aaaaacatgt ccacctatgt tgactataaa
     5581 atcaacctgt tggatgaaga tgacagtttc tacgctgcca acggcatctc taccgatgat
     5641 attgtcgctt taggtctggt ttatcagttc taaatcctcc tgcccgctgt tatggcgggc
     5701 tttttctgct tattcttcct cctttgatct aaattaaaaa tgtgaactcc gtcattacac
     5761 aaaaagtgtc atctggcgtt acactttatg cggatactaa aacaggaggt tttatgaaca
     5821 gaacgattct tgtccctatc gatatttcag attcagaatt aactcaacgc gtgattagcc
     5881 acgttgaggc agaggcaaag attgatgatg cagaggttca tttcctgacg gtaatacctt
     5941 cactgcccta ctatgcctct ctgggtttag cgtattccgc agaattaccg gcaatggatg
     6001 acctgaaagc ggaagccaaa tcgcaactgg aagagatcat taaaaaattt aaactgccaa
     6061 ccgacagagt gcatgtccat gttgaggaag gctcgcccaa agaccgcatt ctggaattgg
     6121 cgaagaagat ccccgctcat atgatcatca ttgcttccca tcgaccggat atcaccactt
     6181 atctgctcgg ttccaacgcc gcagctgtag tgcgtcacgc agagtgctcc gtgctggttg
     6241 tgcgctgaca ctaacgcccg cacattgctg cgggcttttt gattcatttc gcaaatgtgc
     6301 tgacattttc ccctctaatc cgtaccatac acgccacagt ttttatatca gatttcttat
     6361 gctgagcgtt ccggcatgac gcatactctt ctgatgccat ataacgaatt gagtcgcttt
     6421 taaatgtcgc aaaatcaaga aattagtaag aaagaacaat acaacctgaa caaattacaa
     6481 aaacgtctgc gtcgtaacgt gggcgaagcc attgctgact tcaatatgat tgaagaaggc
     6541 gatcgcatca tggtttgcct ctccgggggt aaagacagct ataccatgct ggagattctg
     6601 cgcaatttgc agcaaagcgc gccaatcaat ttttcgctgg tggctgttaa cctcgatcaa
     6661 aagcaaccgg gcttcccgga acacgttctg cccgagtatc ttgaaaagct gggcgttgag
     6721 tacaagattg ttgaagagaa cacttacggt atcgtgaaag agaagattcc agagggcaaa
     6781 accacttgct cactgtgttc tcggctacgt cgcggtatcc tttatcgcac cgcaacggaa
     6841 ctggggacga cgaagatcgc gttgggtcac catcgtgacg atatcctgca aacgttgttc
     6901 ttaaatatgt tctacggcgg caagatgaaa ggtatgcctc cgaaactgat gagcgatgat
     6961 ggcaaacata tcgttattcg tccactggcc tactgccgcg agaaagatat tcagcgattt
     7021 gccgatgcaa aagcgttccc gattattccg tgcaacctgt gcggttcaca gcctaacctg
     7081 caacgtcagg tgattgctga catgttgcgt gactgggata aacgttatcc agggcgtatc
     7141 gagacgatgt tcagcgcgat gcagaatgtg gtgccgtcgc atctgtgcga taccaacctg
     7201 ttcgatttca aaggcattac ccacggttct gaagtggtta acgggggtga tctggcgttt
     7261 gatcgcgaag agatcccact acaaccggcg ggctggcagc cagaagaaga tgaaaatcag
     7321 ttggatgagt tacggctgaa tgtggttgaa gtgaaataac caagatagcg cccgatgcgc
     7381 aagcgtatcg ggctactctt atggaggccg gataagacgc ggccagcgtc gcatccggca
     7441 atcccgaata agatgtttac tcttgcaccc ggcaattcaa catttcatta ttttaataac
     7501 cgcacccggc acgtttttcc tttaatcttc ccgccctgta actgtttcca tgctttatga
     7561 gcaacagcct gacggaccgc gacatagaca tgcgccggat gcacggcgat tttgccaata
     7621 tctgcgccat caagcccgat atctcctgtc agtgcaccta atacatcacc cgggcgcatt
     7681 ttggcttttt tcccgccatc gatacacaac gttgccattt ctgcttccag cgtcacaatg
     7741 gaactattag ctggcggcgt ttgccagtta agttttatct gcaacatgtc agaaatgata
     7801 ttggcccgct gtgcttcttc cggagcacag aaactgatcg ccagaccgct atttcctgca
     7861 cgagctgtac gaccgatgcg atgtacatga acttcagggt cccacgccag ctcaaagttc
     7921 accaccagct caagcgattt aatatccaga ccacgcgcag caacatcagt cgcaaccagt
     7981 acacgggcgc taccgttagc aaaacgtacc agggtctgat cgcgttgctc cagatcgccg
     8041 tgtaacgaca atgcactttg ctctacttca ttcagcgcgt cgcagacagc ctggcaatct
     8101 tttttggtat tgcaaaacac cacacaagag gatggctgat gcaagcttaa taaccgttgc
     8161 aacagaggaa ttttgccttt actggatgtc tcataaaatt gttgttcaat gggtggcaaa
     8221 gcatctgttg agtcaatttc aatcgccaaa ggatcgcgtt gcactcgtcc gctgattgcg
     8281 gcgatggctt ccggccaggt tgccgaaaac agaagcgtct gtcgagatgc aggcgcaaaa
     8341 cggatgacat catcaatggc atcgctaaat cccatatcca gcatgcggtc ggcctcatcc
     8401 atcaccaaag tattcagcgc atccagtgat accgtgcctt tttgcaagtg atccagcaaa
     8461 cgacccggcg ttgccacgat aatatgcggc gcatgttgca gcgaatcacg ctgcataccg
     8521 aacggttgac caccgcacaa cgtcaaaatt ttggtatttg gcagaaaacg cgccagccga
     8581 cgcaattcac ctgccacctg atccgccagt tcacgcgtag gacacagcac taaagcctgg
     8641 gtttgaaata gcgacgcatc aatttgctgt aacaagccga ggccaaaagc cgccgttttg
     8701 ccgctgccgg ttttcgcctg cacgcgaaca tcttttccgg caaggatcgc cggaagcgcg
     8761 gcggcctgca ccggcgtcat ggttaaataa cccaactcat taaggttcgt gagttgggcg
     8821 ggaggcaaaa cattcagggt agaaaaagcg gtcacaatct attctcgtgg tcatcgacgc
     8881 aaagttagca ggcgcgtatc ctcgcagatc tacgctcacg atgcgacaat ttaatcggtt
     8941 cttcatcggg tggtgggtca ggcatgggtt gcgggcgagg gatcggatcg ggcactggaa
     9001 caggatcgcc aggaatcggt tcagggacag gaatttgcaa ataaataagt gtcgtcatat
     9061 ttccctccgg tcattgggtg gactcttaaa gggtagacgc tgataaataa caggcaaaaa
     9121 aaagccgact catcaaagtc ggcgtcgtac gaatcaattg tgctatgcag taattcaaaa
     9181 aaggaagtaa gacaatatgg agcgcaacgc ccatcgcttg acgttgcatt cacctgcaag
     9241 ggagatattg ccctgaatgg gtagagagtt tattgacttc gctcaaactt tgcggcgttt
     9301 ttgtatacag acagccggaa aaattgcttt tgttacaacc atttactacg gtgcaaccat
     9361 aaagcaacac caccaataag aacaactaac agaatacaaa aaattgaaaa tccgaattgc
     9421 cacccgccgc cagggatccc accaaggttg acgccaaata acccggtcag aaaggtactg
     9481 ggtaaaaaga ccattgccat caacgacatt gtataggtac gacgagctaa attttcctgc
     9541 atcacctgag cgatttcatc cgccatcacg ccagtccgtg ctatacaggc gtcgatttcg
     9601 tcaaggccgc gcccaaggcg atcggcaata tcctgcatcc gacggcgttg gtcatcgctc
     9661 atccacggca aacgttcact ggcaagacga gcataaacat cacgttgcgg tgccatatag
     9721 cgacgcatca caattaattg tttgcgcagc agagccagga atccacgcgg tggaatttgc
     9781 tgatcaagga gattatcttc aaggtcgata attttatcgt gcagctgctc gataaattca
     9841 ctggaatgat cggtcaacgc atcgcacaca tccaccagcc atcccccgca atcggtcgga
     9901 cccgtgccct cttccagatc gctcaccaca tcgtccagcg ccagcacttt gcgttgtcgg
     9961 gtcgaaacaa ttaaccgccc gtccatatat acacgcatgg cgaccagttg atcggggcgt
    10021 tcatcggtgc tgccgtttat acagcgcaat gtaatcagcg tgccttcacc gaaacggctg
    10081 actcggggcc gtgtgctctc gcccgccagc gcatcacgta cgttattggg aagcagcggt
    10141 gttgtcgcca gccattgggc gctatcatgg tgtacataat taaggtggag ccagcaggga
    10201 tgcgcttcat caatcacatc tgtattttcc agcggtttaa cgccgcctct accatccagc
    10261 atccaggcaa atactgcatc cgggacatta acgtccgatc ccttaatcgc ttccacagtg
    10321 cctccatcat caacgcatta ttttgtagtc tagccttctg gccctgttac gcaacatctc
    10381 atcaccccat taccctgaaa tgattaataa aattctgtct aaattgaata caaaaagcaa
    10441 aatgcttttc cgtatacaaa ccgtgtgaag tgttaaatag cgtctatcat tatcagaatt
    10501 atctgatcat atgacgtggc ttttttgcga tcggatagca acaaaaattg ataaaaataa
    10561 cgggatctca atgattacgc acaacttcaa taccctggac ttactcacca gtcctgtctg
    10621 gattgtttcg ccctttgagg aacagttaat ttatgccaat agcgcggcgc gactgttgat
    10681 gcaagacctc acgtttagtc agctacgaac cggaccctat tccgtctcct cacaaaaaga
    10741 actgccgaaa tacctctccg atctgcaaaa ccaacacgat attatcgaaa tcctcactgt
    10801 tcagcgtaaa gaagaggaaa cagcattaag ctgtcggctt gttttgcgag agctgacaga
    10861 aacagaaccg gtgattattt tcgaaggtat cgaagcgccg gcaacgctgg gtttaaaagc
    10921 cagtcgctcg gcaaattatc agcgcaaaaa acaaggtttt tatgcgcgct tttttctgac
    10981 taactctgca ccaatgttgt tgattgaccc gtcacgagat ggacaaatcg tcgatgctaa
    11041 cctcgccgcg ctcaatttct atggttataa ccatgaaacg atgtgccaga aacataccta
    11101 ggaaataaat atgcttgggc gtcgcgtcat gcctatcatg catgaaattt cgcatttacc
    11161 cggcggtcat aaccctttga attttgttca taaactggcg gatggatcta ctcgtcatgt
    11221 gcagacctat gccggaccga ttgaaattta tggcgacaag ctcatgttat gtattgtgca
    11281 tgatattact gagcaaaaac ggctggagga gcagctggaa catgctgctc accatgacgc
    11341 gatgaccgga ttactgaatc ggcgacagtt ttatcacatt acggaaccag gccaaatgca
    11401 gcatctcgcc atcgctcagg attacagctt gttgctcatc gacaccgatc gttttaaaca
    11461 cattaacgat ctctatgggc attctaaagg tgatgaggtg ttatgcgccc tcgcccgccc
    11521 cctcgaaagt tgcgctcgca aaggcgattt ggtgtttcgt tggggaggcg aagagtttgt
    11581 cttattgcta ccaagaaccc cactggatac cgcgctttcg ctggctgaaa ctatccgcgt
    11641 aagcgtggca aaagtgagta tttcgggctt accacgcttt accgtcagca ttggtgtggc
    11701 gcatcacgaa ggaaatgaaa gcatcgatga actgtttaaa cgcgttgatg atgctttgta
    11761 tcgggcgaaa aatgatggac gcaaccgcgt gctggcggca taagccgcgg atgcgtctcg
    11821 agatcaacga ctgcgcttag cgtggcgctc ccagttttct tgcttcgcct gcgccgtttt
    11881 acgtagtgcg acgtaacacg ccccgctgcc gccatgatgc ggtagcgcgg tgcaatatgc
    11941 ctgaacatca tcaaattcgg tcagccagcg cgccacatag ctgcggacaa tattggcatg
    12001 tgatttatct tcccgccctt taccatgaat aatcagcaca ttacgcaaac catccgccag
    12061 ggcttgttga atgaaactga acaccatttt gcggcactct tccaccggct ggcgcaaaag
    12121 attcaggctc gcctgttgcg gatatttacc actgcgcagc ttatccagca ccccatgctg
    12181 caacccttcc tgccgaaact ccagcggctg acttagcggg atgatgtcga gaaatccggt
    12241 agtgaggaaa ttatcaagct gcagcgtgtc gatacgctgc ggcgcacgtt ggttacgcgt
    12301 tggatgccag tggacatctg tagcacgttt cagcggctgg acatcttcca tggcgtcaag
    12361 aaacagcgat ttgtcgtcaa ggttcatgta acctcctatg caccttcaca cgtcgtgatt
    12421 cagacgggtt aattgttcat acttactgca tcgttccctt cacagattga aacaatactc
    12481 tacttcatac ctgtgagtat ctgcgcaggc ggggcatttt atactagcct gttagcagta
    12541 tcgaacagtt tagcccgagt ttaattcagc acaggctccg atcaggatga tttattcata
    12601 gcaggaacca acaaaaatgc gataaataac agaccactga ttagcagaaa ggtaccgaaa
    12661 atgccatagt ttgcgcctcc ctcagcatca attgactcaa taatttggtt gaagccagta
    12721 tttccgatgt ctggtcactt ctactgcgat taaaaaataa agcacttctg gaaatgaagt
    12781 cattcaccgt atggggaaca gaaaccattt tttcacccat tctcgaaaaa tgaaaattag
    12841 ttcgattatt gagaatagag ccacacaggg caataccaat cgcgccccca agattacgtg
    12901 ttaaattaaa tacaccagag gctaacttca gccgttcttt tggaattccc cccagcgtta
    12961 atgttacgat gggggccatc gcaaactgct gcgaaatccc tctaattgcc tgtggaaaca
    13021 gtaattcctg ccatccccat tcgtgggtta taggggtaaa aagatacatt gaaaacacaa
    13081 atccgcccag ccccgccatc aacagccatc gcagatttat ttttttactt aaccagaagt
    13141 aaaagggaac agaaaataac tggaaaatac ctgttgtgca aacggcaaaa cctatctctt
    13201 cggcatttaa tccgcgaatc tgccccaaaa acacaggtat taaatataca gtagagaata
    13261 tcccaacccc tccagaaaaa gaaaaaaaac atcccagagt gaaattttta tccttaaaag
    13321 catgaagatc cattatcgga ttggatattg taagtgtacg cgcagcaaat aaaataaaac
    13381 tcaccaatgc cagaactgat gtcagcaaaa tagtattatc atccagccat ccccagcgcg
    13441 ctccttcttc aagggtatat tccagacatc ccagagtcat ggccaacagg ataatactcg
    13501 gataatcagc aactttcagt aaagagagat caggtttatc aaaattaacc agaaaaggaa
    13561 tactcaaaac taaatatatg ccaggtaata tattgatata aaatagccat cgccagtcga
    13621 ggttatcagt gagccagccc cccagagttg gccctagcgt cggtgacagt gaggccagcg
    13681 cactgactac cgcagcagcc aaccccaact ctttaccctg gtaataaata aacgccatcg
    13741 tgaaaactaa agggatcatg gatgcgccag cagctccctg taaagcacga aacaaaatca
    13801 tgatctgaat attccatgca agtccacacg cgatactcat taaagtaaat attcccgcgg
    13861 atagagtaaa aagccaacga gtggaaaata cccgtgacag ccacccagag agcggaataa
    13921 caataatctc agcaattaaa tagctggtct gaagccaggc catttcgtct ttacctgcgg
    13981 acagaccacc acctatttca tttagggatg acgatacaat ttgaatatca agctgagcaa
    14041 taaataatcc catacacata ctggcgaaag caatatgttt acgcatagac atcatctctc
    14101 atcaacactg acggtgacgg acagtcccgg gcgaagaagt tgctttaatt cctttgcgtc
    14161 gtcaaatgca atccgtaccg ggacacgctg aacaattttg gtgaaattgc cagtggcatt
    14221 ttctatcgga atcaagctga aactggctcc tgtcgctggg gaaaggcttt caatgtgacc
    14281 gtgaaacact tcccctttga gaatatcagc acggatctca gccttcattc ctggcttcat
    14341 tcccgctatt tggttttctt tataatttgc atcaacccac aactcactga ctggtactag
    14401 tgaaactaac gatgtgccgc cttccaccca actgcctgta tgggcgctgc ggtttgcgac
    14461 aatgccatca atcggcgcac gaaccacggc agcctgacgg ctgcaaattg aaaac
//
LOCUS       DAEEUE010000096        14252 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.096, whole genome shotgun sequence.
ACCESSION   DAEEUE010000096 DAEEUE010000000
VERSION     DAEEUE010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14252
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.096"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..106)
                     /locus_tag="KLK84_003166"
     CDS             complement(<1..106)
                     /locus_tag="KLK84_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HBE2010399.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(147..602)
                     /gene="csgB"
                     /locus_tag="KLK84_003167"
     CDS             complement(147..602)
                     /gene="csgB"
                     /locus_tag="KLK84_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HBE2010400.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            1355..2005
                     /gene="csgD"
                     /locus_tag="KLK84_003168"
     CDS             1355..2005
                     /gene="csgD"
                     /locus_tag="KLK84_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HBE2010401.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            2010..2399
                     /gene="csgE"
                     /locus_tag="KLK84_003169"
     CDS             2010..2399
                     /gene="csgE"
                     /locus_tag="KLK84_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HBE2010402.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            2424..2840
                     /gene="csgF"
                     /locus_tag="KLK84_003170"
     CDS             2424..2840
                     /gene="csgF"
                     /locus_tag="KLK84_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HBE2010403.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            2867..3573
                     /locus_tag="KLK84_003171"
                     /pseudo
     CDS             2867..3573
                     /locus_tag="KLK84_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(3706..4197)
                     /locus_tag="KLK84_003172"
     CDS             complement(3706..4197)
                     /locus_tag="KLK84_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBE2010404.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4299..4853)
                     /gene="ycdY"
                     /locus_tag="KLK84_003173"
     CDS             complement(4299..4853)
                     /gene="ycdY"
                     /locus_tag="KLK84_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HBE2010405.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4877..5614)
                     /gene="ycdX"
                     /locus_tag="KLK84_003174"
     CDS             complement(4877..5614)
                     /gene="ycdX"
                     /locus_tag="KLK84_003174"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HBE2010406.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(5669..6607)
                     /gene="ghrA"
                     /locus_tag="KLK84_003175"
                     /pseudo
     CDS             complement(5669..6607)
                     /gene="ghrA"
                     /locus_tag="KLK84_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6842..6929
                     /locus_tag="KLK84_003176"
     tRNA            6842..6929
                     /locus_tag="KLK84_003176"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6876..6878,aa:Ser,seq:gga)
     gene            complement(7079..7360)
                     /locus_tag="KLK84_003177"
                     /pseudo
     CDS             complement(7079..7360)
                     /locus_tag="KLK84_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(7642..8646)
                     /locus_tag="KLK84_003178"
     CDS             complement(7642..8646)
                     /locus_tag="KLK84_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HBE2010407.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(8718..9278)
                     /locus_tag="KLK84_003179"
                     /pseudo
     CDS             complement(8718..9278)
                     /locus_tag="KLK84_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(9363..9560)
                     /locus_tag="KLK84_003180"
     CDS             complement(9363..9560)
                     /locus_tag="KLK84_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010408.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(9572..10063)
                     /locus_tag="KLK84_003181"
     CDS             complement(9572..10063)
                     /locus_tag="KLK84_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010409.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(10060..10434)
                     /locus_tag="KLK84_003182"
     CDS             complement(10060..10434)
                     /locus_tag="KLK84_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HBE2010410.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(10481..10855)
                     /locus_tag="KLK84_003183"
     CDS             complement(10481..10855)
                     /locus_tag="KLK84_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HBE2010411.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(11018..11239)
                     /locus_tag="KLK84_003184"
     CDS             complement(11018..11239)
                     /locus_tag="KLK84_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HBE2010412.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(11302..11778)
                     /locus_tag="KLK84_003185"
     CDS             complement(11302..11778)
                     /locus_tag="KLK84_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HBE2010413.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(11794..12267)
                     /locus_tag="KLK84_003186"
     CDS             complement(11794..12267)
                     /locus_tag="KLK84_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBE2010414.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(12609..13430)
                     /locus_tag="KLK84_003187"
     CDS             complement(12609..13430)
                     /locus_tag="KLK84_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBE2010415.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(13530..13703)
                     /locus_tag="KLK84_003188"
     CDS             complement(13530..13703)
                     /locus_tag="KLK84_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HBE2010416.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            complement(13823..>14252)
                     /locus_tag="KLK84_003189"
     CDS             complement(13823..>14252)
                     /locus_tag="KLK84_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010417.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
BASE COUNT         3641 a         3490 c         3412 g         3709 t
ORIGIN      
        1 cgccaccacc gtggttaccg ccgccgccgt actgaggaac aacacctgcc agagcgctac
       61 cggagaatac gattgctgca attgctgcta cttttaaaag tttcatgtaa aacccccatc
      121 ggattgattt aaaagtcgaa tggtaattaa cgttgtgtca cgcgaattgc catttgcgac
      181 tgtctctgca ctacaattgc cgttttttga gtaccatact gtgtaatatt tgctttatta
      241 ccagaacctt tctgtataat catcgcagta ttaccataag caccttgcga aatactggca
      301 tcgttggcac tgcccgcctg atcaatatat gcaaggttat aatctcctgt ctggtcaatc
      361 tttgcccgat tgctactacc ttcttgcgca acaaccgcca aaagttttga gcctccctgc
      421 cgtaactgag cactattatt agtcccagct tgaccaatta tggctgcctg attaaatgaa
      481 gacttactca attcatttac cgcgaagtta tattctgaat tagctaaatc ataacctgct
      541 gcggctgcaa tcccaggcgc acccagtatt gttaacatca taaataacaa tttgtttttc
      601 atgttgtcac cctggacctg gtcgtacatt taagaaatta aatcatttca acttggttgt
      661 taacgcaacc tgtattttgt taacgctgcg ttacgatgga aagtatgtct gcggaaatat
      721 ttttaataac tcacccgcgc gttgtatttt tctttttttg ttcataccaa agtattaaaa
      781 cccgcgtaaa tacgctgtta tctacgcaaa aaatattttg ttttctctta aatctccgtt
      841 ttccgctatc aaaaatcacc agacagtcat tcttcttgcc cgtcgctgat tgctgcgata
      901 tgtcttgcgc acaagccgtg acaaacctcg tctttttgca gtaaaaaaat tgtccacgga
      961 ggtgtggaga aaaaacaaaa acgttttaca tgacgaaagg actacaccga aatattttta
     1021 tatgtattat tagtaagtta gcaccatttg tatgattttt taaaattgtg caataaaaac
     1081 caaatgtaca acttttctat catttctaaa cttaataaaa ccttaatgtt aacattttaa
     1141 tataatgagt tacattttgt tacatgttta acacttgatt taagatttgt aatggctaga
     1201 ttgaaatcag atgtaatcca ttagttttat attttaccca tttagggctg atttattact
     1261 acacacagca gtgcaacatc tgtcagtact tctggtgctt ctattttaga ggcagctgtc
     1321 aggtgtgcga tcaataaaaa agtggggttt catcatgttt aatgaagtcc atagtattca
     1381 tggtcataca ttattgttga tcactaaacc ttctttgcag gcgacagctc tcttgcagca
     1441 ccttaaacaa tcgctagcaa ttacaggaaa attacataat attcaacgtt ctctggacga
     1501 tatctcttca ggctctatta ttcttctgga tatgatggaa gcggataaaa agcttatcca
     1561 ttattggcag gatactttga gcaggaaaaa caacaatatc aaaatattgt tgctaaatac
     1621 gcctgaagat tacccgtacc gcgacattga aaactggcct catatcaacg gcgtttttta
     1681 tgccatggag gatcaagaac gtgttgtcaa tgggttgcaa ggcgtcctgc gtggcgaatg
     1741 ctactttacg caaaagcttg ccagttacct gattacacat tctggtaact atcgttataa
     1801 cagcacggaa tcagccctcc ttactcatcg ggaaaaagag atcctgaata agctgcgtat
     1861 cggcgcgtct aataacgaga tcgctcgttc gttgttcatc agcgaaaata cggttaaaac
     1921 gcatctttat aatcttttca agaagatagc cgtaaaaaac cggacacaag cggtttcctg
     1981 ggcaaacgat aacctcaggc gataaagcca tgaaacgtta tttacgctgg attgtggcgg
     2041 cagaatttct gttcgccgca gggaatcttc acgccgttga ggtagaagtc ccgggattgt
     2101 taactgacca tactgtttca tctattggcc atgattttta ccgagccttt agtgataaat
     2161 gggaaagtga ctatacgggt aacttaacga ttaatgaaag gcccagtgca cgatggggaa
     2221 gctggatcac aataacggtc aatcaggatg ttattttcca gactttttta ttcccgttga
     2281 aaagagactt cgagaaaact gtcgtctttg cactgattca aactgaagaa gcactaaatc
     2341 gtcgccagat aaatcaggcg ttattaagta cggacgattt ggcgcatgat gaattctaaa
     2401 taaaaaattg ttcggaggct gcaatgcgtg tcaaacatgc agtagttcta ctcatgctta
     2461 tttcgccatt aagttgggct ggaaccatga ctttccagtt ccgtaatcca aactttggtg
     2521 gtaacccaaa taatggcgct tttttattaa atagcgctca ggcccaaaac tcttataaag
     2581 atccgagcta taacgatgac tttggtattg aaacaccctc agcgttagat aactttactc
     2641 aggccatcca gtcacaaatt ttaggtgggc tactgtcgaa tattaatacc ggtaaaccgg
     2701 gccgcatggt gaccaacgat tatattgttg atattgctaa ccgcgatggt caattgcagt
     2761 tgaacgtgac agatcgtaaa accggacaaa cctcgaccat ccaggtttcg ggtttacaaa
     2821 ataactcaac cgatttttaa gccccagctt cataaggaaa ataatcatgc agcgcttatt
     2881 tcttttggtt gccgtcatgt tactgagcgg atgcttaacc gccccgccta aagaagccgc
     2941 cagaccgaca ttaatgcctc gtgctcagag ctacaaagat ttgacccatc tgccagcgcc
     3001 gacgggtaag atctttgttt cggtatacaa cattcaggac gaaaccgggc aatttaaacc
     3061 ctacccggca agtaacttct ccactgctgt tccgcaaagc gccagggctt acaaaacctg
     3121 cttaacgagc gcaaggttat tcgtgcggca caagaaaacg gcacggttgc cattaataac
     3181 cgaatcccgc tgcaatcttt aacggcggca aatattatgg ttgaaggttc gattatcggt
     3241 tatgaaagca acgttaaatc gggcggtgtt ggggcaagat attttgggat cggtgcagac
     3301 acgcaatacc agctcgatca gattgccgtg aacctgcgtg tcgtcaatgt gagtaccggt
     3361 gagatcctct cctcggtgaa caccagtaag acgatacttt cctatgaagt tcaggccggg
     3421 gttttccgct ttattgacta ccagcgcttg ctggaagggg aagtgggtta cacctcaaac
     3481 gaacccgtta tgctgtgtct gatgtcggct atcgaaacag gggtcatttt cctgattaat
     3541 gatggtatcg accgtggtct gtgggatttg taaaataaag cagaacggca gaatgacatt
     3601 ctggtgaaat accgccatat gtcggttcca ccggaatcct gacagataaa aatgccggaa
     3661 ttaataatcc ggcatttact gtttaagcga aagcgtgagt gcccctcacg ctttattttt
     3721 gatttcctgc tcacggtgag ccgtctttgc actacgcgcc gccagccaca ggccgctgtt
     3781 tttcattgcg taaccaaata ccagcccaag cgccagcgaa ggtaacacca gtttccagtc
     3841 accctgcccg gcaaacgtcg cgcatgcgcc tataaacgtc cccggaacaa atgaaagcag
     3901 cagctgtttg gcctgaatac acatcagaaa cgcgacaata ccggttatga cataaccgag
     3961 aatttccaga tgtggtgcca gtgcactacc gtaaataatg accatcgccc acaccacgcc
     4021 actaagcagg gttgcagcgg agatcgccag ccctttcagc ccaccttgcg ggcaagcaaa
     4081 gtaggccgta cagcccagga agcccgccca gcttagtaag cccagggaaa cagccaccca
     4141 tccccagata ccggagagaa tgcctgttgt gattgcaata gagagaagta tgttcatgcc
     4201 gcgcatgata gcagaaaacg cggccatgaa tgagatcgag cacacatttt atgcaacaac
     4261 aaattatctg ttgcagttat gagtgacgat gatcacattt actcttcaga atcttcttcc
     4321 agctcgtccc acattgcact aatggcatca cgggttagcg gtgccatggt gcgccagaaa
     4381 ggcgtggttg catgggcctc cactttgccg aggaacgcac cacaccaggg taacagatac
     4441 tcactgaaca gtgtttccag tgcttcgctc tcatcttccg ttgactgatc ttccagccag
     4501 gaagccgcaa gcagcaatgt gccgatgtga tcggctggcg tatccgccag tggcatccct
     4561 cgttcggaaa gaaaagcgcg cacttccgct tccgttgcgt cctcaaccca tgcgctacga
     4621 tatggcggca cggcacattc atcgccgata aacaacgcat tgtaatcggc agagacttgc
     4681 gtcatatcac agcttttctg taaacgcgtc agtaactcat cctgctccag tggccagttc
     4741 gcagccagtt tcccctcacg aatcagagta aacagcggca ccagtaaagg atcttgcggt
     4801 tggcggtaat agagcgaacc cagcacacga cagaggatag aaaactcgtt cattaatata
     4861 tttccattac tagtaattaa aggtctgcaa attccgcaat cggtgccata ccgcgagatt
     4921 caaggaagtt cagtaagcgg cgcggagaaa cattcaaaat gcgctctagc ggaaaatcta
     4981 ccgcgtcgag gattttaaga cactcttcaa attcccccat ggtaaacgcg gtgtgagaat
     5041 ccgagcctaa tgccacccaa cctccagcat cacgtaccgc tgcagctacc gcacgacagt
     5101 tgtcttcact gcccttacgt gagtgtaaaa atgaggaatt attgatttcc agcgccacct
     5161 gatgtttcgc ggctgcttca gcaacggctt taacatctat ttcatatttc ggatttccgg
     5221 gatggcttat tatatgcaca ttgccgctgg cgatagtgga gatcatcgct tgtgtattgg
     5281 tcgctttgtc atgtggcgca aaaaccggct catgaaaacc ggcaataatc agatccagcg
     5341 agtcaaacat tttaccgctg cagtcaattt caccatcaac atttttaatg ttagcttcaa
     5401 tgccgcgcag gatccctacc ccatcaacca ctcgcggcca gatacgcatg ttaatgaagt
     5461 gccagtgatg cggcgcatct tccatatccg ggccatgatc ggtgatcgca aaaagtttaa
     5521 tgcccttttg tttggcctgg gcaatgtaat cactcaatgt gctatatgca tgtgtgctgg
     5581 caacggtatg catatgaagg tcgacgggat acataactct ctcctgtatt caattcccgc
     5641 caaggatagc aggaatcctg atgctttatt agtagccacg tgcgcggtcg acttgcccgc
     5701 agaccctctc ccctttttcg agctgggcaa tagtgcgaga aatgtactcc acagcttcag
     5761 cgggacgggt aatcgcggcg acatgtggtg ttatcgtcac gcgtggatgt tgccagagcg
     5821 gactttcagg cggtaagggt tcacgattaa aaacatccag cattgcgcct ttaactttcc
     5881 cgctatccag cgccgcgagc aggtcatctt ccacaacatg aacaccacgc gccaggttga
     5941 ggagatacgc gccatcaggt aatttttcga gtaattgttg attaataatg ccgacggttt
     6001 caggggtatt cggtaacaaa ttaatcaata cccgacattg gctcagaaat gcagacagtt
     6061 cttcccgtcc ggcaaagctt tgcacgccag gccacgattt acgggttcga ctccagcaac
     6121 gcagcggaaa gcgctaggtt tgcagactct gagcaacttt acagcccagt acgcctgcgc
     6181 ccaaaatgcc gatggtaaaa tcttcccgat gatattcagg cagcggttgc caatgcgaac
     6241 tattttgctg gatgcgataa tcgtcaaaac gtcgaaacca atgcagcacc tgactgacag
     6301 catattcctg catttgctcg cccataccgg tatcttccag gcgaaaaagt ggaacagaag
     6361 ggttcagcat ttcagggtgt gcctgtagct tgctcaaaat agaatcaaca ccggccccga
     6421 gtgcgaacac cgctttaaga tcgcgccctg ccagcatttc aacaggagga tgccagacta
     6481 aaacataatc agcagagtca ttatctccgc ttttccatgc cctgactctt gcctgaggaa
     6541 tagctttgcg cagtgcctca atccaccatt gggtatcgaa cgttgggtga taaaagatga
     6601 tatccatact gactcccgaa aagcgttgtg cgaaatttat tcgcacttat cgttatgatc
     6661 tacaaaggcc accagcataa caaatccgtg gtcggtggca aaaaaagcag atttcgctta
     6721 ttaaaaccac acattgattg aatgtttgaa taaacgcgcg attttttcaa aaagtttgtt
     6781 gacctcaggt catgatttcc ctaaattagc gcccgttcca gcaagacagg aacgacaatt
     6841 tggtgaggtg tccgagtggc tgaaggagca cgcctggaaa gtgtgtatac ggcaacgtat
     6901 cgggggttcg aatccccccc tcaccgccag attataagtg catgttattg tttaacatgc
     6961 gcttttttat ttttccttcc agacagtatg ccatcgggta tgccatctga aaccggttta
     7021 tatgccccgc gatcatatct tatcggacaa tttccgatct ttgagcatac tggcaaagct
     7081 atactgccag catatcagga tagtgttttg cgataatatc attcagtctg tcaaccagtt
     7141 ctggttttct gaacgtgata tgcgcggagc ccttcttaaa gtatctgatg ctgaacatct
     7201 catcttcata gctctcttta ccctgaacag actgaatgtg atcatccaga cgaagggtga
     7261 tgtttccccg gttgtcgggg attggtttac cgctgaacag gtgcaacatt ctttccagat
     7321 cggcgagtcg gtcagcctgt tgtccggtga taagatgaaa tctatggtca cccccgtttt
     7381 tgcaacactg atttttgatt aatggttggc ttgcttaaat ctatccggcg tctctgtggg
     7441 caaggatgcc cgcgccccga tgagttccgc acctgataat cctaaaaatc ccctcggctt
     7501 cagtgagcca tttcatctgt taggttttta atcaggtcga tttgccgttc atgtcatcaa
     7561 taatcagtct tcgcaaaacc agaaaggtaa cttgtatctt attgtttaaa ataaactaat
     7621 tgagttatat cagcatctat atcaggcctg atattctttt ttctgagtag tgattgccca
     7681 ggcaattcga gccagtttat ttgccagagc gcaggtaacc acattcgaat gcttcttgtt
     7741 gagttgctcc cgaacccact cagcaagttt tccatgcttg cgttcaagtc gcattatgaa
     7801 tgagcgggca cactgaacca gtaaccggcg taaattttta tcacctcgct tactgatacc
     7861 caacagagtc gattttcctc cagtgctata ctgccgggga acaagtcctg tggaagctgc
     7921 aaaatccctg ctgcatgaga actgtttccc gtcaccaagt tgcgacgata gtaagctggc
     7981 agtaatcgga ccaacaccag gaattgtcat aatgcgctga gcggtatcat cagcgttgat
     8041 tgactgactt aattctgatt ccagctctgc tatttgctca acaaggtaaa agtagtgggt
     8101 atgcaacctc accaaaaggc ggctcagata ttcggggatt tcgtgttcag ccaacacgag
     8161 agacaaccgt tttattacag catcacctat cggcaggctg attccaaatt ccagcaagaa
     8221 accgtggatc tggttggttg ttttcacctt atctctgatg agcgattctc tgacccggtg
     8281 taacgccctc atggcctgct gggtctcagt tcttggctgg acaaaacgca ttgatggtcg
     8341 tgaagccgct tcgcatatgg cttctgcatc aacaaaatca ttcttattac ttttgacaaa
     8401 cgggcgaacg aactgtggag aaatcagctt agtttcatgc ccaaaatcag agatacagcg
     8461 agccataaag tgtgcacccg cacaggcttc cattacaaca actgagggcg gacatgtcgc
     8521 cagaaactgc atcagtttag tacgggtaaa ttttttacgc agaagagcct taccagactt
     8581 gtcttgacaa tgaatgtgga aggagtgctt tccgagatcg ataccaataa gtgtgatgtt
     8641 ctgcatgatg gtctctccgt taaagggatt taccttaaca gcgtactgct gttaaggtgg
     8701 gggtgaccat atcattatcc ccaccggtcc catctcacca gattgttgac gataatttta
     8761 ctgccaaatt tacaggggca attggttttg taattccagt tcagccctct gaagacgttg
     8821 atcacccctc gctcaaacac ctcatcctta ttctggtgca gttgttcaaa tgtgctcaga
     8881 atgttcgctt cgctgatttc cggaaaatta tcgtactcca gtgaccggta ccatgtatca
     8941 cgggcctgcg tatccattaa tgacagcatc ccggaacgtt gcatcaggtc acgccagatt
     9001 tcgcgatcaa gattccgggt aatggctttc attgctgttt cgggtttctc catcagccag
     9061 cagccgcagc gaaaatcctg tttcatggcc cagtccgggg cagttttacc tccgatgctt
     9121 tccgtcagca ccgagatttc agccagttgt cggataagtg tgtcgatttg caacagtgta
     9181 ctctttcgta cagccagaat gtggcgaatg ctggttgagc agataacgtc cgtatgcccg
     9241 ttgaccactt ccggacggga gattgtgata tctgacataa tcgatattcc tttttgcaga
     9301 taaatgaaaa cgccagccct gtgcaggctg gcgtcagagg aatgaagtct ggtgagtgag
     9361 cttcaccggt tctggtgcag aagctgcaga tgacgcaggg tacggacatc ctcccgggcc
     9421 tgttcgatac agggcaacag tgctgctgaa tccgttttat cctcatcgtt gtcaaagata
     9481 attcccgatc cgcagtcgat attgtcctgc agccacgcaa tcagaatatc cagcgctgtt
     9541 tccgtggtta atgatttcat gttgttcctg gttaccagtc gaaagtgggt aaacccggca
     9601 ggcattcagc gctggcatcc agatgaatga gactgacgcc ataacgccgg atgagtgtga
     9661 cgaccagacg acggaacgtt tttgacagcc ccaggcgttt aagccgcaga accgggtatg
     9721 accacgcatc ggtacgtaac agatatccgc tacctgtaaa atgaatccat tctgattcac
     9781 cgaagtcact ggtctggtgt gacagcgagt acaaccaggc gttgtccgtt tccgtgatat
     9841 gtgcggtact gcagcgtatg ccggtaaggg tagcaaatgg tggcgtgcat tctgctgagg
     9901 tctggtcaga ttcatcaaca acacgaaggg agtagccgtt atcgcagacc atgttaatca
     9961 gttcagcgag ctcaatacca tcgacgtcaa cgacaatgcg ccctatgttc agtgcctgta
    10021 cgttaacgct gtcggcttcc agtgtcaggg ccagtttcat catttcgcct ccggatactt
    10081 acccagggta atgttattta ccgttctgta attgtcgcgg gtcatcaggc cggtcgcccg
    10141 gcgagcccgg aggatatcaa tactgtttat taactgagag cggggacagg tgctgaatcc
    10201 cggctggtcg gtacgcacca gcgcgtattt ttccacgaga aaattaactg catcacacag
    10261 tgaaatgcct gcctcaatat gctgctcaat cacacgttca tcgacaaacg gtgtgtcatt
    10321 cagtgtgagg ccataatgct ggtccagcag tcggatgagc agtgtctgcc agatggtgac
    10381 gggagacggg cagcgagata cctcccgtac atgtgtgtcg gataatgttt tcatcttaag
    10441 ttctctgtaa ctggtagtgc taaaggtgag agcttcgggg ttatgaggtg gttgcgggtg
    10501 ctggtgtcgg ataaacagcc agataaacgt aaccacagga gccgagggtg tcggcttcgc
    10561 aggtcagccc ttttgcatac agcgtgacgg tatgctgata tcggggattc agttcaccgc
    10621 tggtgagcat gagttccagt tgtttcatca gcagcggaaa ggcctggtcc agatggtatg
    10681 catctgcgtc gctgaagcgg cctctgatac cggcgcggtc ggcaaggtaa tacaggcggt
    10741 taccctcttg caccagacga gcgccaaaac agggcctcac tgtgcaggga agcccccacc
    10801 aggggcggtc gtgattgtca tcgggatgag ttatcccgga gagtttgtct gacacgataa
    10861 aatcctctct gaaaagtggt taagaatggt ttaacgatga caggtcagcc ggcgcgctga
    10921 ccggtttcgg tgaagatgta tgcattgatg agaatggcct catccacagc ggcgtctgag
    10981 gtcatctggt cgtactcgtt tcccgggtga cggaacatca gttcagggtg acactcatca
    11041 gccggacata cgggccaaaa ctgtccttac ggcgttctgc aaacacggca agaacgccgg
    11101 gaatatcctg cacttcacga ccagtatacg cttcagcact gccgtgccag cgatacttac
    11161 cggtacagaa cggaaaaaga cgggatgcag gatgctgttg gtgaatacgc atggcttcac
    11221 cacgggtgat gattctcatg atgtgatacc tctgaagaca gaagataaaa gtgaaaacag
    11281 gtgtgatgtg gttgtgacgg gttaaagcag accatgttcg gcaaaggaga aaacctggtt
    11341 gccaccgact atcagatggt ccggcacccg gatatccacc aggcccagtg cctgtaccag
    11401 acgttcggtg ataagccggt ctgccttact gggtgtgact tcaccggacg ggtgattgtg
    11461 cgccagcacc acggcagcgg cattgtggta cagggcgcgt ttaatcactt cccggggatg
    11521 aacttccgta cggttgatgg tgccggtgaa gagggtttca ccggcaatca gctgattctg
    11581 gttgttcaga tacagcaccc ggaactcttc ccgctccagt cccgccatgt tcagaatcag
    11641 ccattcccgt gccgcacggg tggaggtgaa ggccacgccg ggttcatgaa gatggcggtc
    11701 cagggtttgc agggcccgca gaatgagact gcgctcgccg ggcgtcatgt ttccgggcag
    11761 aaaggaaagc tgctgcattg tccctgcctc cattcagtcg atgatgcgca taatggcgct
    11821 gcattccgga tgctgcaggg cgtaatcccg cagccggtaa taatggaccg tcatggcata
    11881 acactccgta cgacaggcat gatgactgta cgtcatcaga caggcagcaa tgccggcggc
    11941 ttccgggctc atttcagcgc ggttaccgtt catggcattg aacagtaccc atgtttcgtc
    12001 atcatcgtca tccggttcgg gtgccataaa tgccccgccg ttgttcaggg tgtacagatt
    12061 ccagatacca ccgcagtagt cttcacacag acggtccatc cagccgaaga cacggggttc
    12121 cagggtgacc cactgtggaa tgaggccaaa atgctgcggc cagaagctga tgcgctgttc
    12181 atcagggacg ggagtggcaa ccagttgttg caggccagtc tccggtgcac aggccgaagc
    12241 ggtggtgttc tgagacaatg ttttcatgta tgaaatcctt gtaatgtgaa taaatgagag
    12301 ggagtaaagg tcgggagctg gcaacatcac gttcatcact gaccgtgatg acccggtaat
    12361 atcagggatg aatgatattc ggctgaaatt ccggacagcc gtacgcgtaa ccgttccggt
    12421 cacagacgga tggccagatg ccattgcctg tatactcagt ctgcacagac tgatttctcc
    12481 gggacggtaa agcagacggg tacaccaggc gcacaggtca tcatcagaaa tggaacgaca
    12541 ccccggcagc gcctccgggg cgtcgggtga ttctggtgcc tgaatcacgc cttcagggac
    12601 atgctgcctc agcgcaggct ctccagcagc gtttctgcca ttacccacaa tgcgcggttg
    12661 agcttaatat cggtgtcgat gttgtgaatg gcacgggtgt ggatacgttt tccttttgca
    12721 ctgcggccgg aaatcccgcc tttcagcata ttctcctgga tggtctgata tgcgctccac
    12781 aggtctttac cgtaatcctc cctgcgtcgt ggtgtcagaa tgtcagcggt ggtgactggc
    12841 tgatgttcgt caccataacg gtaagtcagt gccgcctgtg ccagcgcctg gcgtgccggt
    12901 ggcggcagga ccagcgactg catggcatca cgcttctcct ctatccggtc aaacacgccc
    12961 accacctcgt aagccccttc aataactttc tccaccacat ttccccggtg tggaacacgc
    13021 aattctccca gagactgacc gcagaccagt ccattggtac aaatggcacg aaaatatccg
    13081 ggtaacatct ggtagctgga tgaaccgtca tgagagttga gcagaataat ttcagggaca
    13141 tgctgaccgg ttatctgtcc ggcccgccgc agacgcagca tgtgttttgt gtattcccgg
    13201 cgacccgggt cacgcacacg tgtctggcag gcgaagaatg gctgaaagcc ttcccgctgc
    13261 aggctttcca gtacggtgat ggtgggagtg tacgcatagc gtttactgcg ggaagtatgc
    13321 cggtcttcac cgaaaatact gggaacatag tgcatcagtt cttcgtgtgt cagcggacgg
    13381 tcacggcgta tctggtttgc agcataacca aaacgactgg ctagtcgcat aatttgctcc
    13441 ttatcggtgg ttaagattta ctggtgtaat aaatgaaaaa gccacgtctc ccggagaaga
    13501 cgcggcctga tggacgaaat taatgaggtt tattgtctga gaagaccgtc actggcgaac
    13561 cttccggcgt cagtctctgc gttgcggata acccgacgga aatgagttcc ctggtcatcg
    13621 tcgataaaca cattgcggta agctgttgtg atgacaacag cctgctcgcg tgagaaaggg
    13681 acttcgggca ataaacgtga catacaacca gacctgtatt tggttccctg aaaatataag
    13741 tccctgtcat catggcagag ccgtatttac ttttgatatc aataaattaa taacgcgaag
    13801 atgaaccgca taacttacgc ctttaatttg ttttgtttac gtagtccctg accaggtacg
    13861 cgaattcgtg aacgccagtt tcaccaaacc aaatcaggta accagggtcg atgttgtaat
    13921 agtaaccaac gactttatgc gtttttgggt tatagagtgt tacgatatag tcccagccgc
    13981 aggcgtgggc tttacaaccg ccaccttcta cccaggtcgt tccgttaatg tttacaggat
    14041 acgcaccacc atcaggacca tccatatagc cgtcttcccc aaacattccg tttaacccaa
    14101 cagggtttaa tgctttgtcg atagcagctc tgtaggtagc gtcaccttcg tataactcgt
    14161 acgttgttgg acatgcacca ggctggtcag cccgggcttt tgagtcgcat ttaagtgtct
    14221 gatttacagt ggcggcgttt gtgtcaggac tt
//
LOCUS       DAEEUE010000097        14118 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.097, whole genome shotgun sequence.
ACCESSION   DAEEUE010000097 DAEEUE010000000
VERSION     DAEEUE010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14118
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.097"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(197..2872)
                     /gene="adhE"
                     /locus_tag="KLK84_003190"
                     /gene_synonym="adhC"
     CDS             complement(197..2872)
                     /gene="adhE"
                     /locus_tag="KLK84_003190"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBE2010418.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            3349..3996
                     /gene="ychE"
                     /locus_tag="KLK84_003191"
     CDS             3349..3996
                     /gene="ychE"
                     /locus_tag="KLK84_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HBE2010419.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            4734..6365
                     /gene="oppA"
                     /locus_tag="KLK84_003192"
     CDS             4734..6365
                     /gene="oppA"
                     /locus_tag="KLK84_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HBE2010420.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            6451..7371
                     /gene="oppB"
                     /locus_tag="KLK84_003193"
     CDS             6451..7371
                     /gene="oppB"
                     /locus_tag="KLK84_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HBE2010421.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            7386..8294
                     /gene="oppC"
                     /locus_tag="KLK84_003194"
     CDS             7386..8294
                     /gene="oppC"
                     /locus_tag="KLK84_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HBE2010422.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            8306..9319
                     /gene="oppD"
                     /locus_tag="KLK84_003195"
     CDS             8306..9319
                     /gene="oppD"
                     /locus_tag="KLK84_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HBE2010423.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            9316..10320
                     /gene="oppF"
                     /locus_tag="KLK84_003196"
     CDS             9316..10320
                     /gene="oppF"
                     /locus_tag="KLK84_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HBE2010424.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(10373..10702)
                     /locus_tag="KLK84_003197"
     CDS             complement(10373..10702)
                     /locus_tag="KLK84_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HBE2010425.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(10737..12197)
                     /gene="cls"
                     /locus_tag="KLK84_003198"
     CDS             complement(10737..12197)
                     /gene="cls"
                     /locus_tag="KLK84_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBE2010426.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            12340..12513
                     /locus_tag="KLK84_003199"
     CDS             12340..12513
                     /locus_tag="KLK84_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HBE2010427.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(12568..13821)
                     /gene="kch"
                     /locus_tag="KLK84_003200"
     CDS             complement(12568..13821)
                     /gene="kch"
                     /locus_tag="KLK84_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HBE2010428.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         3647 a         3285 c         3491 g         3695 t
ORIGIN      
        1 cccagagaag tcagtgggtt acgcagagaa tcgtaacggg ataccagatc aagagcctgt
       61 ccaatgtgca taaaaaaatc cggaaacaag tgagcgtttc cggattctta cacagccact
      121 ggatcggtca actgatcctt aactgatcgg cattacccca gaaggggccg tttatattgc
      181 cagacagcgc tactgattaa gcggattttt tcgctttttt ctcagcttta gccggagcgg
      241 cttctttttt cgctgcagtt tcaccttcta cataatcacg accgtagtag gtatccagca
      301 gaatctgttt cagctcggag atcagcgggt aacgcgggtt agcgccggtg cactggtcat
      361 cgaatgcatc ttcagacagt ttatccacgt tcgccaggaa gtctgcttcc tgaacgccag
      421 cttcacggat agatttcgga atacccagtt cagctttcag cgtttccagc catgccagca
      481 gtttctcgat cttagcagca gtacggtcgc ccggtgcgct cagacccagg tggtcggcaa
      541 tttcagcata acgacggcga gcctgcggac ggtcatactg gctgaatgca gtctgcttgg
      601 tcgggttgtc gttcgcattg tagcgaataa cgttacaaat cagcagggcg tttgccagac
      661 cgtgcggaat atggaactgg gaacccagtt tgtgcgccat tgagtgacat acacccagga
      721 aggcgttcgc aaatgcgata cccgcgatag ttgctgcact gtgaacacgt tcacgcgcta
      781 ccggattttt agacccttcg tggtaggacg ctggcagata ttctttcagc agtttcagtg
      841 cctgcagagc ctgaccatca gagaactcag atgccagtac agaaacataa gcttccatgg
      901 cgtgagttac tgcgtccaga ccaccgaaag cacacaggga cttcggcatg tccataacca
      961 ggttggcgtc gacaatcgcc atatccgggg tcagcgcata gtccgccagc gggtatttct
     1021 gaccagtagc gtcgtcagtt acaaccgcaa acggagtgac ttcagaacct gtaccagaag
     1081 tggtggtgac agcgatcatt ttcgctttca cgcccatttt cgggaacttg tagatacgtt
     1141 tacggatatc cataaagcgc agcgccagct cttcgaagtg agtttccgga tgttcgtaca
     1201 taacccacat gatcttcgcg gcgtccatcg gggaaccacc acccagcgcg ataatcacgt
     1261 ctggtttgaa ggagtttgcc agttctgcac ctttacgaac gatgctcagg gtcgggtctg
     1321 cttctacttc gaagaagact tcagtttcaa cgcctgctgc tttcagtacg gaagtgatct
     1381 gatcagcata accattgttg aacaggaagc ggtcagtcac gatgagcgca cgtttgtggc
     1441 catcagtaat cacttcatcc agcgcgattg gcagggagcc acggcggaag tagatagatt
     1501 tcggaagttt gtgccacaac atgttttcag ctcgcttagc aacggttttc ttgttgatca
     1561 ggtgtttcgg accaacgttt tcagagatgg agttaccacc ccaggaacca caacccagag
     1621 tcagggaagg tgcgagtttg aagttataca ggtcaccgat accaccctga gaagccgggg
     1681 tgttaatcag gatacgagct gttttcattt tctgaccgaa gtaagaaacg cgagccggtt
     1741 ggttatcctg gtcagtgtac aggcaagagg tatgaccgat accgcccata gcaaccagtt
     1801 tctctgcttt ttctaccgcg tcttcgaaat ctttagcgcg gtacattgcc agagtcgggg
     1861 acagtttttc atgtgcgaac ggttcgcttt catcaacaac ggtcacttca ccgatcagaa
     1921 tcttggtgtt ttctggtaca gagaagcctg ccagttcagc aattttatag gctggctgac
     1981 caacgatagc cgcgttcagc gcaccatttt tcaggataac atcctgaaca gctttcagct
     2041 ctttaccctg caacagatag ccgccgtggg ttgcgaaacg ttcacgtact gcgtcataaa
     2101 cagagtcaac aacaacaaca gactgttcag aagcacagat tacgccgttg tcgaaggttt
     2161 tggacatcag tacagatgca actgcacgtt tgatatcagc agtttcatca ataacaactg
     2221 gagtgttacc cgcgcctaca ccgatagctg gtttaccgga gctgtatgcg gctttaacca
     2281 tgcccggacc accagtcgcg aggatcaggt tgatgtctgg gtggtgcatc agtgcgttag
     2341 acagttcaac agaaggttga tcgatccagc cgatcagatc tttcggagca ccggcagcga
     2401 tagcagcctg cagaacgata tcagccgctt tgttggttgc atcttttgca cgcgggtgcg
     2461 gggagaagat aatggcgtta cgggtcttca ggctgatcag cgatttgaag atagcagttg
     2521 aagtcgggtt agtggtcgga acgataccgc aaataatacc gattggttca gcgatagtga
     2581 tggtaccaaa agtgtcgtct tcagacagaa caccacaggt tttttcatct ttataggcgt
     2641 tgtagatata ttcagaagca aagtggtttt tgatcacttt atcttcgacg atacccatgc
     2701 cggattcggc aacggccatt ttcgcgagtg ggattcgagc atctgcagca gccagagcgg
     2761 cggcgcggaa gattttgtct acttgctctt gagtgaaact ggcatattca cgctgggctt
     2821 tttttacacg ctctacgagt gcgttaagtt cagcgacatt agtaacagcc ataatgctct
     2881 cctgataatg ttaaactttt ttagtaaatc atctgctcga atacgagagt atagtcagtg
     2941 cggtgatgat ttgcttaacc gatgaaaatc aaaagcttac tcgcgctcac actcactgtg
     3001 atttactaaa agagtttaaa cattagagtt attatctcta atgcgtcact tccaggtggc
     3061 gtaagcaaga ttactcactt ctgggtactg attacgtgat ccaaatcaaa tttttgcaaa
     3121 gctgacacct ttcagcatcg cttttcgcca ttatagctaa cagttaataa attgtagtat
     3181 gatttggtgg ctacattagc atgttttgca caactagata acaataacga atgatagcaa
     3241 ttttaagtag ttaggaggtg aaaaatgctg tcaaaaggcg tattgtcagc gcgtcttttc
     3301 aaccttattt atggctaaca ttatccggct tttgcttcgg agcgaaccgt gattcagacc
     3361 ctttttgatt ttcccgttta cttcaaattt ttcatcgggt tatttgcgct ggtcaacccg
     3421 gtagggatta ttcccgtctt tatcagcatg accagttatc agacagcggc agcgcgaaac
     3481 aaaactaacc ttacagccaa cctgtctgtg gccattatct tgtggatctc gctttttctc
     3541 ggcgacacga ttctacaact ttttggtata tcaattgatt cgttccgtat cgccgggggc
     3601 atcctggtgg tgacgatagc gatgtcgatg atcagcggca agcttggcga ggataaacag
     3661 aacaagcaag aaaaatcaga aaccgcggta cgtgaaagta ttggtgtggt gccattggcg
     3721 ttgccgttga tggcggggcc aggggcgatc agttctacca tcgtctgggg tacgcgttat
     3781 cacagcatta gctatctgtt tggtttcttt gtggctattg cattgttcgc tttatgttgc
     3841 tggggattgt tccgcatggc accgtggctg gtacgggttt tacgccagac cggcatcaac
     3901 gtgattacgc gtattatggg gctattgctg atggcattgg ggattgaatt tatcgttact
     3961 ggtattaagg ggattttccc cggcctgctt aattaattcc tttcaaatga aacggagctg
     4021 ccatgctccg tttacttcgt cattattttt actttgttcc cgcgcagtta tcaaaagcaa
     4081 aaggaatagg taaaaatatt cttctcaaat tacagttagt tataaggatt tccttaactg
     4141 tttctcctca ccatcatgtt attttcgcca catcataatc ctgggcttgc tgaagaataa
     4201 ttgaaatgat attagtaatt ccactgcctt tggtagagga aagtgctaaa taataatcaa
     4261 ttgttaaatt attgtgcatt tcactactgg aactgtaatc agaaaagata gacatgctta
     4321 gccaatctct atttgattga attgaaagat gtttgttaag gcatggatgc aagctataga
     4381 ttctgatacg gtcaataaaa gagaatcgct taacaatttt gcaaaatgta ttggcgagta
     4441 agaaccgcat ttggtacttt ccgggcaacc gccagacgat tctttattgg taatgagaat
     4501 aattaacaat taaagagcgt cgcgaaagaa taatgtgtct cgacagggga gacacagtac
     4561 gaatcgacat aaggtgatcg tctgaatcac cagaataaat aaagtcggtg atagtaatac
     4621 gtaacgataa agtaacctga cagcagaaag tctccgagct tgtgcagggt cccaatccgg
     4681 gattacacat gctggttaat accagtaatt ataatgaggg agtccaaaaa acaatgacca
     4741 acatcaccaa gagaagttta gtagcagctg gcgttctggc tgcgctaatg gcagggaatg
     4801 tcgcgctggc agctgatgta cccgcaggcg tcacactggc ggaaaaacaa acactggtac
     4861 gtaacaatgg ttcagaagtt cagtcattag atccgcacaa aattgaaggt gttccggagt
     4921 ctaatatcag ccgagacctg tttgaaggct tactggtcag cgatcttgac ggtcatccag
     4981 cacctggcgt cgctgaatcc tgggataata aagacgcgaa agtctggacc ttccatttgc
     5041 gtaaagatgc gaaatggtct gatggcacgc cagtcacagc acaagacttt gtgtatagct
     5101 ggcaacgttc tgttgatccg aacactgctt ctccgtatgc cagttatctg caatatgggc
     5161 atatcgccgg tattgatgaa attcttgaag ggaaaaaacc gattaccgat ctcggcgtga
     5221 aagctattga tgatcacaca ttagaagtca ccttaagtga acccgttccg tacttctata
     5281 aattacttgt tcacccatca acttcaccgg tgccaaaagc cgctatcgaa aaattcggcg
     5341 aaaaatggac ccagcctggt aatatcgtca ccaacggtgc ctatacctta aaagattggg
     5401 tcgtaaacga acgaatcgtt cttgaacgca gcccgaccta ctggaacaac gcgaaaaccg
     5461 ttattaacca ggtaacctat ttgcctattg cttctgaagt taccgatgtc aaccgctacc
     5521 gtagtggtga aatcgacatg acgtataaca acatgccgat cgaattgttc cagaagctga
     5581 aaaaagagat cccggacgaa gttcacgttg atccatacct gtgcacttac tattacgaaa
     5641 ttaacaacca gaaaccgcca ttcaacgatg tgcgtgtgcg taccgcactg aaactaggta
     5701 tggaccgcga tatcattgtt aataaagtga aagcgcaggg cgacatgccc gcctatggtt
     5761 atactccacc gtatactgat ggcgcaaaat tgactcagcc agaatggttt ggctggagcc
     5821 aggaaaaacg taacgaagaa gcgaaaaaac tgctggctga agcgggttat accgcagaca
     5881 aaccgttgac catcaacctg cggtataaca cctccgatct gcataaaaag ctggcgattg
     5941 ctgcctcttc attgtggaag aaaaacattg gggtaaacgt caaactggtt aaccaggagt
     6001 ggaaaacgtt cctcgacacc cgtcaccagg gcacttttga tgtggcccgt gcaggctggt
     6061 gtgcagacta caacgaacca acttccttcc tgaacaccat gctgtcgaac agctcgatga
     6121 ataccgcgca ttataagagc ccggcctttg acagcattat ggcggaaacg ctgaaagtga
     6181 ctgacgaggc gcagcgcaca gctctgtaca ctaaagcaga acaacagctg gataaggatt
     6241 cggccattgt tcctgtttat tactacgtga atgcgcgtct ggtgaaaccg tgggttggtg
     6301 gctataccgg caaagatccg ctggataata cctatacccg gaatatgtac attgtgaagc
     6361 actaatggca atacgtgggg caggagtgtc ctgctccacg gtgtctgatt tttatcgcat
     6421 tacagaaggc acaggccaga aggtagggca atgttaaaat ttattctacg tcgttgtctg
     6481 gaagcgattc cgacgctatt tattcttatt actatttcgt tctttatgat gcgcctcgcg
     6541 ccgggaagcc cttttaccgg cgaacgtact ttaccgccag aagtgatggc caatatcgaa
     6601 gcgaaatatc atcttaatga tccgatcatg acacagtatt tcagctacct gaaacaactg
     6661 gcgcatggcg atttcggtcc atcgtttaaa tataaagatt attcggtcaa tgacctggtg
     6721 gcatccagtt ttcccgtttc tgccaaactg ggagccgccg catttttcct tgcggtaata
     6781 ctgggtgtta gtgctggcgt tattgccgca ttaaaacaaa acaccaaatg ggactatacc
     6841 gtgatggggc tggcaatgac cggggttgtt atccccagtt ttgtggttgc gccattatta
     6901 gtcatgatat ttgcgatcat tttgcattgg ctgccgggcg gtggctggaa tggtggggcg
     6961 cttaaattca tgatattgcc gatggtggcg ttgtcactcg cctatatcgc cagtattgcg
     7021 cgtattaccc gtggctctat gattgaagta ttacactcca actttattcg tactgcccgg
     7081 gcgaaagggt tacctatgcg gcggatcatt ttacgccacg cattaaaacc tgctctgtta
     7141 cccgtgctct cctatatggg ccccgcattt gtcggcatta ttaccggttc tatggtcatc
     7201 gaaaccattt atggtttgcc ggggattggg caactgttcg ttaatggcgc attgaaccgt
     7261 gactattcct tagtgttaag cctgaccatc ctggttggtg cgttaaccat tttgtttaat
     7321 gccattgtcg atgtgctata tgcggttatc gacccgaaaa tccgttactg atactggagc
     7381 tcgcgatgat gttaagtaag aaaaacagcg agacgctgga aaatttcagt gaaaagctgg
     7441 aggtcgaagg gcgcagcttg tggcaggacg cacgtcgacg ttttatgcat aaccgtgcgg
     7501 cggttgccag tctgatagtg ctggtgctga tcgcgttatt tgtaatcctg gcaccgatgc
     7561 tttcccagtt tgcctatgac gatactgact gggcgatgat gtccagcgcc ccggatatgg
     7621 agtccggtca ctactttggt actgactcat ccggtcgcga cctgcttgtg cgcgttgcga
     7681 ttggcgggcg tatctcactc atggtcggtg ttgctgcggc actggtggca gtggtcgtgg
     7741 ggacacttta tggttcgctt tccggttatc tgggcggtaa agtggattcg gtaatgatgc
     7801 gtctgctgga aatcctcaac tccttcccat tcatgttctt cgtcattttg ctggtgacct
     7861 ttttcggcca aaacatcctg ctgattttcg tggcgattgg catggtttcc tggctggata
     7921 tggctcgtat tgtgcgtggg caaaccctga gtctgaagcg caaagagttt attgaggcgg
     7981 cacaagttgg cggtgtatcg acgccgggca ttgttattcg ccacattgtg ccgaacgtac
     8041 tcggtgtggt ggtggtctac gcatcgctac tggtgcccag catgatcctc tttgaatctt
     8101 tccttagctt cctggggttg ggtacgcaag agccgttaag cagctggggg gcattgctga
     8161 gtgatggcgc gaactcgatg gaagtctctc catggttact gttgttccca gcgggattcc
     8221 ttgtggtgac actgttttgt ttcaacttta tcggcgatgg cttgcgtgat gccctcgacc
     8281 cgaaagatcg ttaaggagtg cagccatgag cgtaattgaa actgcaactg tgccgctcgc
     8341 acaacaacag gctgacgcac tgctgaacgt gaaagatttg cgtgtcacct ttagtacccc
     8401 ggacggcgac gtcacggcgg tcaatgattt aaatttttcc ctacgtgccg gagaaacgct
     8461 gggcattgta ggtgagtctg gttcgggtaa atcgcaaact gcatttgcgt tgatgggcct
     8521 gttggctgcc aacggacgca ttggcggatc ggcaaccttc aatgggcgtg aaatcctcaa
     8581 tttgccagag cgtgaactca ataaactgcg cgctgaacaa atctcaatga tttttcagga
     8641 cccaatgact tcgttgaatc cctatatgcg cgtcggtgag cagttgatgg aagtgctgat
     8701 gctgcataag aacatgagca aagctgaggc gtttgaagag tcggtgcgga tgctcgatgc
     8761 ggtaaaaatg ccggaagcac gtaaacgcat gaaaatgtac ccgcacgaat tttctggcgg
     8821 catgcgtcag cgagtcatga ttgcgatggc attgctatgt cgacctaagt tgctgattgc
     8881 ggatgaaccc actacagcgc tggacgtcac cgtacaggcg cagatcatga cgctattgaa
     8941 tgaactgaag cgggaattta atactgccat cattatgatc acccacgatc ttggcgtggt
     9001 ggcggggatc tgtgacaaag tgctggtaat gtacgccggg cgcacgatgg aatatggcaa
     9061 tgctcgcgat gtcttttatc aacccgttca tccttattct atcggtttgc tcaacgcggt
     9121 gccgcgtctt gatgcggaag gtgaaacaat gttgaccatc ccgggtaatc cgccaaactt
     9181 gctgcgatta ccgaaaggtt gcccgttcca gccacgttgt ccacatgcga tggaaatttg
     9241 tagtagcgca ccgccgctgg aagagtttac gcctggccgt ctgcgtgctt gctttaaacc
     9301 ggtggaggaa ctgttatgaa tgctgtaact gaaggaagaa aagtcctcct tgaaatcgcc
     9361 gatcttaaag tgcactttga aatcaaagat ggcaaacagt ggttctggca accgccgaaa
     9421 acgctcaaag ccgtcgatgg tgtcactctt cgcctgtatg aaggggaaac attaggtgtg
     9481 gtaggggaat cgggatgcgg taagtccacc tttgctcgcg ccatcatcgg tttggtcaag
     9541 gcgaccgacg gtcatgttgc ctggttaggt aaagagttgc tgggcatgaa gcccgatgaa
     9601 tggcgtgccg ttcgcagtga tattcagatg attttccagg atccgttggc atcgctaaac
     9661 ccgcgtatga ccatcggcga gatcatcgct gaaccactgc gtacttatca tccgaaaatg
     9721 tcacgccagg aagttcgcga gcgcgtgaag gcgatgatgc tgaaagtcgg gttattgcct
     9781 aacctgatta accgctatcc gcatgagttc tccggtgggc agtgccagcg tatcgggatt
     9841 gctcgtgctc ttattcttga accgaagctg attatctgcg atgagccggt ttcggcgctg
     9901 gacgtgtcaa ttcaggcgca ggtggttaac ctgctccagc agctgcaacg tgagatggga
     9961 ttgtcattaa tttttattgc tcatgacttg gccgtggtaa aacacatttc cgatcgtgtg
    10021 ttggtgatgt atctcggcca tgcagtagaa ctggggacct atgatgaggt ctaccacaat
    10081 ccactacatc cttacaccag ggcattgatg tcggcagtcc ccatacctga tccggatctg
    10141 gagaagaaca aaaccatcca gttactggaa ggggaattac cgtcgccgat caacccgcct
    10201 tccggttgtg ttttccgtac ccgttgcccg attgccggtc cggagtgcgc caaaacacgt
    10261 cctgttctgg aggggagttt cagacacgcc gtttcttgcc tgaaagtcga tccgctttaa
    10321 aaacaataag ggctgacagt tgtcagccct ttttcacgct aaaagcgatt atttattccc
    10381 gccagatgat atggcaaagt ttgtgatctt tttcgcggca taacagaatg cgggcgaaaa
    10441 catcgttgat ttcaccatct tcactgtccg ccagaccaat caccacttcg gcaaaaaagt
    10501 ccgggttcaa atcgaaatca acatgttcct gccagtcttc cgccggatcg aataactccg
    10561 cgccgccgcg ctcttcaaac tgaagattga acagcagaac atcagctgga tcgagattgt
    10621 cggcagccag ttcgagaaaa atatcgtagg cctgctcaag cgtttcatct tcagtcaggc
    10681 gattgtttag atccatatcc atagtcacta cctgtttaac ctctgttggc gacgttttac
    10741 agcaacgggc tgaagaagta aaacagtcgc tcggcgacac gttgccataa tggacgtttt
    10801 agccataaac gggcatcgag cagacgtgaa cgcgaaatat aatcgtcctg aacggcggca
    10861 aggtctgcac caaaaccttt atcgtcgatt gccaaggtaa tctcgaaatt cagccacaga
    10921 ctacgcatat caaggttaac ggtgccaacc agacttagtt cgccatcgac cagcacgctc
    10981 ttggtatgca gtaacccgcc ttcaaactga taaattttaa ccccagcagc aagcagttcc
    11041 gtaaagaatg cgcgactggc ccagccgacc agcatcgagt cattttttcg cggaaggata
    11101 atactgacat ccaccccgcg ctgcgccgcc gtgcaaatcg catgaagtaa atcatcgctt
    11161 ggcacaaagt agggcgtggt catgatcaaa tattcatgcg ccgaataagc cgcagtcaat
    11221 aatgcctggt gaatgagatc ttccggaaag ccggggccag aagcaattgt gtgaatggtg
    11281 tgaccgctgg cctgttcaaa cggcataata ttgacatctg gtggtggcgg cagaatacgt
    11341 tttccggttt caatctccca gtcgcaggaa taaataatcc ccatcgcggt ggcgatgggg
    11401 ccttccatac gcgccatcag atcaatccat tgccctacgc ccgcatcttg tttgaagtag
    11461 cgaggatcga ccatattcat gctgccggtg tacgcgatgt aattatcgat catgatcatc
    11521 ttgcgatgtt ggcgcaggtc catacggcgt aaaaacacac gcatcagatt gacctttaag
    11581 gcttcgacca cttcaatacc ggcattacgc attagctcgg gccacgggct gcggaaaaaa
    11641 gccacactcc cggcggagtc gagcatcaat cggcaatgaa taccacgccg tgcagccgcc
    11701 attaatgatt cagccacctg gtccgccatg ccgccgggct gccagatata aaacaccatc
    11761 tcaatattat ggcgcgcgag ctggatgtcg cggattaacg cctgcatcac atcatctgac
    11821 tcggtcatca gctgtagctg attccctttg accccagcga tcccctgacg acgctcgcaa
    11881 agcttgaata atggcgcagc gacactgcta ttttcttcgg cgaagatatg cttacaggct
    11941 ttaaggtcgt taagccattt tgcggtggaa ggccacatcg ctctggcgcg ctcagcgcgg
    12001 cgtttgccta aatggagctc gccaacggca agataggcaa taattccgac taacggcaga
    12061 atgtaaataa tcaacagcca ggccatcgcg gagggaactg cgcgtcgttt cattagaatg
    12121 cgtaaagtta cgcctgcaat gagcaaccag tatcccagaa tggccaacca actcaccaac
    12181 gtataaacgg ttgtcataga ttagaaatcc ttttgaaagc gcattgttat gagtttacgc
    12241 atatggattc atctggcaaa taaaaacgcg ggaaaagcac tggtcagcgt cgggggcggg
    12301 ggtataatga ccattctgtt attgcataga gtagttaaca tgaagcggag tagaacggaa
    12361 gtggggcgct ggcgcatgca gcgtcaggct agccgacgta aatcgcgttg gcttgagggg
    12421 caatcgcgcc gaaatatgcg tatccacagc atcaggaagt gcattctaaa caaacagcgt
    12481 aactcgttat tgtttgcgat ctacaatatc taaatgtact gggcaccatt gcggtgccca
    12541 ataagtttac tcttccgtct ttaaactcta tttttgcgcc gattctttac tgtcagcttt
    12601 cgtttcttgt tcatcgttat cgctgaaaat accatgacct gagttcaaca acattgaaac
    12661 gagcatatca ttattaatct cttcaccatt taaaactcgc gccagaattt cgctgccaaa
    12721 cagttgcggt gagagaatga tatccggatg taccatctta atcttattca ggtttttgct
    12781 atcactgacg gcgagaactg ttttgacatc actgctcata tcttttgccg agagtacaac
    12841 aaacgcgtta tctgcatcgt tatcactcag cgccagaatg gcccggcaac gatcgattcc
    12901 cgctttcttt aatactgaac tgtcattact gtcaccgggg ataacatcag cgttatcgcc
    12961 taagcgttgc tcaagttgct tgatatcatc ttcaggcaag ttgctgataa ccgttacgtt
    13021 ttgtccgcgt tgattcagtt gcaggatcgt attgatggcg agaatcgaat gtccgcaaac
    13081 aataaaatga tctttacgat gcattgtatg attgtttcct tttaaaagtt tgttgaatcc
    13141 cccgcggata agcggaccaa aaattgaggt catagatgtg gcaaaaacgg taatgccgga
    13201 aataatgacc gaaatagtga acaatcgtgc tgattcagaa acagggacaa tatctccata
    13261 gccgacggtt gacatggttt ctatcgaaaa atagaacgcg gtcatcaaac tttctattcg
    13321 cggattaaaa ccttcgctta aataaagcgc accgtaggtt gagtaaaaca gtaatgtcgt
    13381 gaaactaata aatgcaaaaa ttgtcccggc tgcggcgcta ctgtgggaga agtctttgcg
    13441 cagtatcagc aaaaacacca gcgtaaaaat gcaaaatcca atactaaatt tcagccaggg
    13501 ataaaaatgc agggtgtaaa ttagcgctat caacaacaaa atgatactga ttgcccatgc
    13561 gagctttgcc cggaacagta agccaatgga gttcaggaca agaaagacgc cgagcataaa
    13621 tagtggggcg tttgccagca aggagagatc caactctgaa aatgctttga tatggaaaat
    13681 atcaagcaaa ttgaccgaca taccatagat tgttttaaaa ataagcaatc catttaaaaa
    13741 gacggccagc gcgagaatgt cgtgccgtag ggtcacccat aaatttgttg ctgtttgttt
    13801 gaatgtagcc cagtgactca ctgatacctc tgtaaccaca attttgttta attatcttag
    13861 cgtgcacatt ttctgaacat acatgcagcg cgtgaatgtg ttaaacaata gtagatgact
    13921 ttatccgcag taatcaaagg ttttgagtga gcaactctgc gtcaggaaag ggcttttttc
    13981 ctgaacatgc tcacatcata taaagaaata cagataaagt tattatctgc ttgtggtagt
    14041 gaatgcactg accggctata aggaaaggcc aaacaagaac acggttgcaa aaaccgtgcc
    14101 cttaaatatt ggatctct
//
LOCUS       DAEEUE010000098        13964 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.098, whole genome shotgun sequence.
ACCESSION   DAEEUE010000098 DAEEUE010000000
VERSION     DAEEUE010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13964
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.098"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="KLK84_003201"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="KLK84_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBE2010429.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="KLK84_003202"
     CDS             2219..2767
                     /locus_tag="KLK84_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HBE2010430.1"
                     /translation="MGKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="KLK84_003203"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="KLK84_003203"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HBE2010431.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="KLK84_003204"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="KLK84_003204"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HBE2010432.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLSIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="KLK84_003205"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="KLK84_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HBE2010433.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="KLK84_003206"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="KLK84_003206"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBE2010434.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="KLK84_003207"
     CDS             complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="KLK84_003207"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBE2010435.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9765..12380
                     /gene="pepN"
                     /locus_tag="KLK84_003208"
     CDS             9765..12380
                     /gene="pepN"
                     /locus_tag="KLK84_003208"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HBE2010436.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISPA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="KLK84_003209"
     CDS             complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="KLK84_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HBE2010437.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13351..>13964)
                     /gene="ssuC"
                     /locus_tag="KLK84_003210"
     CDS             complement(13351..>13964)
                     /gene="ssuC"
                     /locus_tag="KLK84_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HBE2010438.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         3551 a         3562 c         3526 g         3325 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga gctgtagact tgattatcat
     2221 gggcaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgttgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gctaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcaa ggatgccgtc aatgctgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agctttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggagcttt gtgaacaccg
     6361 tcatatttcc atagaaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagaatg cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcgggggtg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcttcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tgggggggat acctatgtta cctggcatct gcaatcagac
     8221 aagcagaatt cgcaaatgca gcgaaagatt tcggaattaa aggtaaaaaa gggagccgat
     8281 tagctccctg atgatattaa ctggcttttt taatatgcgg aaggtcgaac gctttgcgca
     8341 gcgcccgaac aaacgcttta tcatggcaga tagttttgcc agggctgtca gaaagtttcg
     8401 ccaccggttt accgttacac tctaccaact taatcacaat attcaggggt tttacctggg
     8461 ggatatcgca ggtcaggcga gtcccaatac caaaacttaa ttgcacgcgg gaagagaagt
     8521 ggcggtatag ctcaaccgct ttgcgtaaat ccagattgtc agagaaaacc agcgttttac
     8581 tctgtggatc aattcccagc ttttcataat gtgcaatggc tttttcaccc cattcaaccg
     8641 ggtcgccaga gtcatgacgc aggccctgat accgactagc gaactcgaca ccgaaatcac
     8701 gcaggaaagc atccatagtg atgcagtcgg ttaatgcaat gccaagttgg tcgggatact
     8761 cttccagcca ggcagcaagt gcagctcgct ggctgttggc tagatccggg ctgatttgct
     8821 gatgtgcctg gaaccattcg tgtgcctgtg ttcccatcgg cgtgagggaa agccgacgcg
     8881 ccagatcgta gttgctggtg cccacgaacc aggattcctg ttgcagacgc ttaacgatgg
     8941 tttcttgtac ttcgcgagaa aaacggcgac gggtgccaaa atccatcaga tggaagcgcg
     9001 acatatcaag accggcggtt aacgccgaga agtcgactaa tttgctttcc agcgtgtcga
     9061 gggcttgcgc aacgtcggcc tgcggtgagc gatagcgatg taccatttca ctgataaccg
     9121 ccagcaaagg aacttcccag aggatgactt cacgccacgg accgcttaag cgaatatcca
     9181 gcttgccatt atcgttggac acggtgactt gttccgggtt aaagcggaac tcgcgtaacc
     9241 agttaagata gtcggcctta aagaaaggca gggcagagag ccactgatat tcatcatcct
     9301 gcaggcgcag gtgctgcatc gcctgaatct gttcacgaat agcatcggca taaataccca
     9361 gcagatcgtc acctcggcaa cgaaactccg ccgcgacatg cacatcgtaa tagtgatgaa
     9421 acacggcttg ctgcatatgc aacttataag catctgtatc cagcaacgag tgcagaacag
     9481 gagaagcgaa ttgtgtcata ggtgcgcagt agcgtcctcg tacaggagcg tttagtacaa
     9541 taaacatctt caggaactgc tggagtatac cttgtttcgc aatttattga accccgatca
     9601 caccatatgc cacctttctg gtcgatggca ttacgcggtg tatgttataa aaatgtagca
     9661 ataaaggcgt ttgtacctga aaagatgaag attctgcata gcgcgattta cgcaacagga
     9721 atagactgaa caccagactc tataaaagat gctaaaggtt atttatgact caacagccac
     9781 aagccaaata ccgtcacgat tatcgtgcgc cggattacca gattactgat attgacttga
     9841 cctttgacct cgacgcgcaa aagacggtcg ttaccgcggt cagccaggct gtccgtcatg
     9901 gtgcatcaga tgctccgctt cgtctcaacg gcgaagacct caaactggtt tctgttcata
     9961 ttaatgatga gccgtggacc gcctggaaag aagaagaaga gggcgcactg gtcatcagta
    10021 atttgccgga gcgttttacg cttaagatca ttaatgaaat aagcccggcg gcgaatacgg
    10081 cgctggaagg gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt
    10141 tccgccatat tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa
    10201 ttattgccga taaaatcaaa tatcccttcc tgctttccaa tggtaaccgc gttgcgcaag
    10261 gcgaactcga aaacggacgc cattgggtac agtggcagga cccgttcccg aaaccgtgct
    10321 acctgtttgc gctggtggca ggcgactttg atgtactgcg cgataccttt accacgcgtt
    10381 ctggtcgcga agtagcactg gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt
    10441 gggcgatgac ctcgctgaaa aactccatga aatgggatga agaacgcttt ggcctggagt
    10501 atgacctcga catctatatg atcgtcgcgg tggatttctt caatatgggc gcaatggaga
    10561 ataagggtct gaatatcttt aactccaaat atgtgctggc ccgcaccgac accgccaccg
    10621 acaaagatta cctcgatatt gaacgcgtta tcggccatga atatttccat aactggaccg
    10681 gtaaccgagt gacctgccgc gactggttcc agctcagcct gaaagaaggt ttaaccgtct
    10741 tccgcgatca ggaattcagc tctgaccttg gttcccgcgc agttaaccgc atcaataatg
    10801 tgcgcaccat gcgcggattg cagtttgcgg aagacgccag cccgatggcg cacccaatcc
    10861 gcccggacat ggtcattgag atgaacaact tctacaccct gaccgtttac gagaagggcg
    10921 cggaagtgat ccgtatgatc cacaccctgc tgggcgaaga aaacttccag aaagggatgc
    10981 aactctattt cgagcgtcat gatggtagcg cagcgacctg tgacgacttt gtgcaggcga
    11041 tggaagatgc gtcgaatgtt gatctctccc atttccgccg ttggtacagc cagtccggta
    11101 cgccggttgt gaccgtcaaa gacgactaca atccggaaac tgagcagtac accctgacca
    11161 tcagccagcg cacgccagcc acgccggatc aggcagaaaa acagccgctg catattccgt
    11221 ttgccatcga attgtatgac aacgaaggta aagtgatccc gttgcagaaa ggcggtcatc
    11281 cggtgaattc cgtgctgaac gtcacccagg cggaacagac ctttgttttt gataatgtct
    11341 acttccagcc ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa ctggaatata
    11401 agtggagcga tcagcaactg accttcctga tgcgtcatgc gcgtaatgat ttctcccgtt
    11461 gggatgcggc gcaaagtttg ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc
    11521 aagggcagcc gctgtctctg ccggtacatg tggctgacgc tttccgcgcg gtgctgctcg
    11581 atgagaagat tgatccggcg ctggcggcag aaatcctgac gctgccttct gtcaatgaaa
    11641 tggctgaact gttcgatatc atagacccga ttgctattgc cgaagtacgc gaagcactca
    11701 ctcgtactct ggcgactgaa ctggcggatg agctgctggc tatttacaac gcgaattacc
    11761 agagcgagta ccgtgttgag catgaagata ttgcaaaacg cactctgcgt aatgcctgcc
    11821 tgcgcttcct tgcttttggt gaaacgcatc tggctgacgt gctggtgagc aagcaatacc
    11881 atgaagcaaa caatatgacc gatgcgctgg cagcgctttc tgcggcggtt gctgcacagc
    11941 tgccttgtcg tgacgcgctg atgcaggagt acgacgataa gtggcatcag gacggtctgg
    12001 tgatggataa atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg
    12061 tgcgtggtct gttgcagcat cgctcattta ccatgagcaa cccgaaccgt attcgttcgt
    12121 tgattggtgc gtttgcgggc agcaacccgg cagcgttcca tgccgaagat ggcagcggtt
    12181 accagttcct ggtggaaatg cttaccgacc tcaacagccg taacccgcag gtagcttcac
    12241 gtctgattga accgctgatt cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc
    12301 gcgcggcgct ggaacagttg aaagggctgg aaaatctctc tggcgatctt tacgagaaga
    12361 taactaaagc actggcttga tacttttgag ccaaaatgcc tgatgcgacg cttgacgcgt
    12421 cttatcaggc ctacaactcc gttgaaattg ctggttttgt aggctggata aggcgttcac
    12481 gccgcatccg gcagccgtgc ccaactgcct gatgcgatgc ttgacgtgtc ttatcaggcc
    12541 tacaagtccg ttgaaaatgc tggttttgtg cgccacatcc ggcatattaa ccatgacgcc
    12601 gaatcagttg ttcagaatgc ccacgccgca ttacccgttg caatacttcc gcttccagct
    12661 ccgctaatcg caccgaccct aatcgacgcg gacgggggat atccactgtc aaatccagac
    12721 taatttttcc ctcttcaatt aacaacaccc ggtcagccat tgctaccgct tcgctcacat
    12781 catgcgtcac caacagtacg gtaaagccgt gctgctgcca aagtgacaca atcaaatcct
    12841 gcatctcgag tcgcgttaag gcgtccagtg ccccgagcgg ttcatcaagc aacaataaac
    12901 ccggtcgatg aatcaacgct cttgccagcg ccacacgctg tttctgcccg ccagaaagtg
    12961 cggcaggcca ttccccggcg cgattctcca gtcctgccgc agccaacgcc cgacgcgcgg
    13021 catcgcgcca ctgaccttta aggcctaacc caacgttatc aatcaccgat ttccacggca
    13081 gcagacgcgc atcctgaaac atcattcgcg tatcatcctg aatttcagcc agcggtgtgg
    13141 tgcccgctaa cacatcgcct gcggttggcg tttccagacc ggccagcagg tgcagcaggg
    13201 tacttttacc accaccgctg cggcccacca ccgccacaaa ctgacctgcc ggaatatgta
    13261 aatccagttg gttcaggacg atattttccg cgtaatgttt gcttactgca ttgagcaaca
    13321 atggtgtgcc ctggttcaga cgagcagtat tcatatcgtg gcctccttca aatgataagc
    13381 cgggttccag cgcaaccaga ggcgctctaa cagctgcgcg ctgacgtcag caagtttgcc
    13441 gagcagggcg taaagaataa tagcgactac caccacgtct gtttgcaaaa actctcgcgc
    13501 attcatcgcc agataaccaa tgccggaatt ggcagaaatg gtttcggcaa caatcagcgt
    13561 cagccacatc aggcccaacg caaaacgcac gccgaccata attgagggca gggcatcggg
    13621 caggatcaca tggataaaca gcggtatgcc ggataatcca tagctacgcg ccatctccac
    13681 cagcccgcga tcgatattac ggatgccatg ccaggtgttg atataaatgg ggaacagcgt
    13741 accgagcgcc accagaaaga ttttcgcgga ttcatcaatg ccaaaccaca aaatcaccag
    13801 cgggatcagc gccagatgcg gcacgttgcg caacatctga attgaggtat ccagcagcct
    13861 ttctccccag cgcgacagtc cgctaatcag tcccagaatc aggcccagcg atccgccaat
    13921 tgaaaagcca atcagcgccc gccaggaact gatcgccaga tgct
//
LOCUS       DAEEUE010000099        13950 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.099, whole genome shotgun sequence.
ACCESSION   DAEEUE010000099 DAEEUE010000000
VERSION     DAEEUE010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13950
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.099"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..231)
                     /locus_tag="KLK84_003211"
     CDS             complement(<1..231)
                     /locus_tag="KLK84_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBE2010439.1"
                     /translation="MSRKTQRYSKEFKAEAVRTVLENQLSISEGASRLSLPEGTLGQW
                     VTAARKGLGTPGSRTLAELESEILQLRKALNEA"
     gene            complement(295..486)
                     /locus_tag="KLK84_003212"
                     /pseudo
     CDS             complement(295..486)
                     /locus_tag="KLK84_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215564.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(627..2660)
                     /gene="metG"
                     /locus_tag="KLK84_003213"
     CDS             complement(627..2660)
                     /gene="metG"
                     /locus_tag="KLK84_003213"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBE2010440.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2792..3901
                     /gene="apbC"
                     /locus_tag="KLK84_003214"
     CDS             2792..3901
                     /gene="apbC"
                     /locus_tag="KLK84_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HBE2010441.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            4163..4444
                     /locus_tag="KLK84_003215"
     CDS             4163..4444
                     /locus_tag="KLK84_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HBE2010442.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4737..5261
                     /locus_tag="KLK84_003216"
     CDS             4737..5261
                     /locus_tag="KLK84_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBE2010443.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            5342..6016
                     /locus_tag="KLK84_003217"
     CDS             5342..6016
                     /locus_tag="KLK84_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBE2010444.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            6032..8512
                     /locus_tag="KLK84_003218"
                     /pseudo
     CDS             6032..8512
                     /locus_tag="KLK84_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            8528..9561
                     /locus_tag="KLK84_003219"
                     /pseudo
     CDS             8528..9561
                     /locus_tag="KLK84_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(9643..9981)
                     /gene="rcnB"
                     /locus_tag="KLK84_003220"
     CDS             complement(9643..9981)
                     /gene="rcnB"
                     /locus_tag="KLK84_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HBE2010445.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(10200..11048)
                     /gene="rcnA"
                     /locus_tag="KLK84_003221"
                     /pseudo
     CDS             complement(10200..11048)
                     /gene="rcnA"
                     /locus_tag="KLK84_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            11170..11442
                     /gene="rcnR"
                     /locus_tag="KLK84_003222"
     CDS             11170..11442
                     /gene="rcnR"
                     /locus_tag="KLK84_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HBE2010446.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      11534..11630
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11665..12453
                     /gene="thiM"
                     /locus_tag="KLK84_003223"
     CDS             11665..12453
                     /gene="thiM"
                     /locus_tag="KLK84_003223"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBE2010447.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            12450..13250
                     /gene="thiD"
                     /locus_tag="KLK84_003224"
     CDS             12450..13250
                     /gene="thiD"
                     /locus_tag="KLK84_003224"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBE2010448.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            13315..>13950
                     /locus_tag="KLK84_003225"
     CDS             13315..>13950
                     /locus_tag="KLK84_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HBE2010449.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVDERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEY"
BASE COUNT         3723 a         3133 c         3421 g         3673 t
ORIGIN      
        1 agcttcattt aacgccttac gcagttgcag aatttcagat tccagttcag ccagcgtgcg
       61 ggaaccagga gtaccgagcc cttttctggc ggcggtaacc cattgtccta aagtgccttc
      121 aggaagagat aatcgggagg cgccttcact gatcgaaagt tgattttcaa gaaccgttct
      181 gacagcttcg gctttgaact ctttagagta acgttgggtt tttctgctca ttattagctc
      241 cttctgatgc cattctattt caggaaggag tgtccgttaa actcaggcta cctcataccc
      301 cttcttcacc ttctccgcaa tcagcttcag ttccgctttt tccgccgccg cagcatctga
      361 aaaacttttt atctgacttt gccctttggt gccaactttt ccccaactga tatgcaactc
      421 gtttccctgt tgctccaccg tccagaattt atgtgatttt tcgtcctgat agataaagtg
      481 tctcattctg catccctgta attttatcca gttgaatgca gatgctacca gtatttatac
      541 gggttagaga gagacaaaat cgcaagaaaa caactttagg aatttataaa gaaaaggcgc
      601 tgtcgatgca gcgccttgaa gggggattat ttcacctgat gacccggttt agcaccggca
      661 tccgggctta gcaggaaaat atctttcccg ccaggaccgg cagccatcac catgccttca
      721 gagataccga agcgcatttt acgtggtgcc aggttagcca ccataatggt gtgacgacca
      781 atcagtgcct gcggatccgg gtaagcagaa cgaataccgg agaagacatt gcgtttttca
      841 ccgccgagat ccagcgtcag gcgcagcagt ttgtcagaac cttcaacaaa ctctgcgttt
      901 tcgatcagcg ccacgcgcag gtcaactttg gcgaagtcgt caaaggtgat ggtttcctga
      961 atcggatcat ctgccagtgg gccagtaact ggcgcggtag tggctttcac ttcttcttta
     1021 gaggcttcca ccagagcttc aacctgtttc atatcgatgc ggttatacag cgccttgaac
     1081 ggattcactt tgtggcccag cagcggttgc tggataccat cccaggtcag ttccgtattg
     1141 aggaaggctt ctgcacgctc ggtcagtttc ggcagtaccg gcttcaggta agtcatcagc
     1201 acgcggaaca ggttgatgcc cattgagcaa atcgcctgca ggtcagcatc gcgaccttcc
     1261 tgttttgcga ctacccacgg agcctgctcg tcgacatagc ggttagccag gtcagccagt
     1321 gccatgattt cgcgcactgc tttaccaaat tcacggcttt cccacgcttc accaatcact
     1381 tcagcggcat cggtaaaggt tttgtacagc tcagggtcag ccagctcgct tgccagcaca
     1441 ccgtcaaaac gcttgttgat aaagcccgca ttacgggagg ccaggttaac cactttgttc
     1501 acgatatcgg cattcacacg ctgaacgaaa tcttccaggt tgagatcgat atcatcaatg
     1561 cgcgaagaga gtttcgcagt gtagtagtaa cgcaggctgt cagcgtcaaa atgattcagc
     1621 caggtgctgg ctttaataaa ggtgccgcga gacttggaca tcttcgcgcc gttcaccgtc
     1681 acatagccat gaacaaacag gttggtcggc ttgcggaagt tgctgccttc cagcatggca
     1741 ggccagaaca ggctgtggaa gtaaacgata tctttaccga tgaagtggta cagctcggcg
     1801 gtggagtctt tcttccagta ttcatcgaag cttacgctgt cgccgcgctt gtcgcacaga
     1861 ttcttgaaag aacccatgta gccaatcggt gcgtccagcc agacgtagaa atatttgccc
     1921 ggtgcgttcg gaatttcaaa accaaagtat ggcgcgtcgc gggagatatc ccactgttgc
     1981 aggccggatt caaaccactc ctgcattttg tttgccacct gctcctgcaa cgcaccgctg
     2041 cgggtccatg cctgcagcat ttcgctgaaa gagggcagat caaagaagaa gtgttcagaa
     2101 tcacgcatca ctggtgtagc accagaaacc accgatttcg gctcgatcaa ttcagtcggg
     2161 ctgtaggtcg cgccgcagac ttcgcagtta tcgccgtatt gatccgggga tttacatttc
     2221 gggcaggtgc ctttcacaaa acggtccggc aggaacatgc ctttttccgg atcgtacagc
     2281 tgagagatgg tgcggttttt aataaaaccg ttttctttca ggcgagagta gataagttct
     2341 gacaactggc ggttctcttc gctgtgcgtc gagtgatagt tgtcatagct gatgttaaag
     2401 cctgcgaaat cagtctgatg ctcctgactc atttcgccaa tcatctgctc cggggtgata
     2461 ccgagctgct gagctttcag catgatcggt gtaccgtggg catcgtcggc gcagatgaag
     2521 ttgacctcgt ggccgcgcat tcgctggtaa cggacccaga catcagcctg gatgtgctcc
     2581 agcatatggc cgaggtggat tgagccgtta gcgtacggca gtgcgcacgt caccagaatt
     2641 ttcttcgcga cttgagtcat agtaggcatt acttcttaat agtgaaaagg ggttagatag
     2701 taccaaatgg gaaaatgtta agtaagtact tctctatatt cttcaggtaa actatcagct
     2761 gatggtttat ctatacaaaa aaggagtcgg gatgaacgaa caatcccagg ccaaatcacc
     2821 agaagccttg cgcgcaatgg tcgccgggac actggccaat tttcagcacc caaccctgaa
     2881 gcataacctc accacgctga aagcgttgca ccatgtcgcc tggatggacg acacgttgca
     2941 tgtggaactg gttatgccgt ttgtctggca tagcgcgttc gaagagttaa aagagcaatg
     3001 tagcgccgaa ctgctgcgca tcaccggggc gaaagctatc gactggaaac tgtcgcataa
     3061 catagccact ctgaaacgcg tgaagaatca gccgggaatt aacggtgtga agaacattat
     3121 cgcggtgagt tccggtaaag gcggcgtggg taaatcgtcc acggcggtaa acctggcgct
     3181 ggcactggcg gcggaagggg cgaaagtcgg cattctggac gccgatatct acggcccatc
     3241 aattcccacc atgctgggcg cagaaaatca gcgtccaact tcgccggacg gtacccacat
     3301 ggcaccgatt atgtctcatg gcctggcaac caattcaatt ggttatctgg tcactgacga
     3361 caatgcgatg gtgtggcgtg gaccgatggc gagcaaggcg ttaatgcaga tgttgcagga
     3421 aaccttgtgg ccggatctcg actatctggt gcttgatatg ccgccaggca ccggtgacat
     3481 tcaattgacg ctggcgcaaa acattccagt aactggtgca gtcgtggtca ccaccccgca
     3541 ggacatcgcg ttgatcgatg cgaaaaaagg cattgtgatg ttcgagaaag tcgaggttcc
     3601 ggtgctcggt attgtcgaga acatgagtgt gcatatttgc agtaactgtg gtcaccatga
     3661 accgattttc ggtaccggtg gtgcagagaa actggctgaa aaataccata cacagctgct
     3721 ggggcagatg ccgttacaca tctccttacg tgaggatctc gataaaggca cgccaacggt
     3781 tatcagccgt ccggagagcg aatttaccgc tatctaccgc cagctggctg accgcgttgc
     3841 agctcagctc tactggcagg gtgaagtcat tcctggcgag atttccttcc gcgcggtcta
     3901 acgcctatta accattctgg ttattcttca tacaccgcag agatgttata ttgatgcggt
     3961 gtatttgttt ttgccgcatt aatgaacact tatttaatac tgcgaatatt ctgcggacct
     4021 tcgtccgaaa ctctacaaag tcgagtgatc gtacaggcaa tgcttcatta tcatccttcg
     4081 tgctgtgtag tggatgtaca gctatagttt gttatgcact cattctatcc aggcataacc
     4141 tttactacag ggataacgtt caatgaataa gtattggctg tcaggaatta tttttcttgc
     4201 ctatggattg gcttctcccg cagtctcttc agagactgcc acattaacga ttaatggcag
     4261 gatcagtccc ccaacatgca gtatggctat ggttaatagt caaccacaac agcattgtgg
     4321 tcagttaacg tataatgttg acacccgtca tcaggtttct tcgcccgtca aaggtgttac
     4381 gacagaagtg gttgttgcag gtagcgatag caaacgtcga atcgtgttaa atcgctacga
     4441 ttaatttggc aacctgattt taattgcatt tagaaagacg aaatcattta tataaatgat
     4501 ttcgtctttt ttattgatat caacaatacc atttacataa atctacatta attaatagat
     4561 gtgataaata tttaatattc ttaagtattt gttgtattta tattgcattg gttaatttgt
     4621 tttaaatcaa taaaacaata tgtttacatt gcggcagctt ttttgatgtg agaaatttgt
     4681 ttccaagaga tacagactct taacaaccaa aaataaattt tattaaggaa catattatga
     4741 aacgttcaat tattgctgcc gctgtctttt cttctttttt tatgagcgct ggagtatttg
     4801 ctgcagacgt tgataccgga acattaacca ttaaaggaaa tattgcagaa tctccgtgta
     4861 aattcgaagc gggtggtgat tcagtaagta ttaatatgcc gactgtacca accactgtct
     4921 ttgaaggtaa agctaaatat tctacctatg atgatgcagt cggtgtaacc agcagcatgt
     4981 taaaaattag ctgcccgaaa gaagttgctg gtgtaaaact ctcgttgatc acaaacgaca
     5041 aaataaccgg taacgataag gcgatagcca gtagcaacga taccgtaggt gataacagcg
     5101 atgtcctaga tgtttctgca ccttttaaca ttgagagtta taaaacagcg gaaggtcaat
     5161 atgctattcc gtttaaagca aaatacctga aactgacaga taactcagtg caatcaggtg
     5221 atgtgttatc ttctctggtt atgcgtgtgg cgcaggatta ataaataaaa ttgcaagttt
     5281 ctccaggtaa taaacatggc tgctatcccc atggcggctt tttaatttaa gaggcattaa
     5341 gatgaaagga ttattatctt tactcatttt ttctatggtc cttcctgcac atgccggaat
     5401 tgttatctac gggacgcgca ttatttaccc ggcagaaaat aaagaagtta tggtgcagtt
     5461 gatgaaccag ggaaatcgtt cttcgctgct gcaggcgtgg attgatgatg gcgatacgtc
     5521 gttaccacca gaaaaaattc aggttccttt catgttaacg ccaccagtgg caaaaatagg
     5581 ggcaaattcc gggcagcaag taaaaatcaa aattatgccg aataaactgc ccactaataa
     5641 agaaagcatt ttttatctga atgttctgta tattccacca aatagtccag agcaagaagg
     5701 caagaatgca ctgaagtttg cgatgcaaaa cagaattaag ttgttttacc ggccagcggg
     5761 tattgctccg gtaaataaag cgacctttaa aaagttactg gtaaatcgca gtggcaatgg
     5821 attggtgata aaaaatgact cagctaattg ggtgacgatt tcggatgtca aagctaataa
     5881 tgttaaagtc aattatgaaa ctattatgat tgccccctta gaaagtcaga gtgttaatat
     5941 caaaagtaat aatgcaaata actggtatct gaccattatc aatgaccacg gcaactatat
     6001 tagtgacaaa atttaattgc aggagctgcc tatgttgaga atgaccccac ttgcatcagc
     6061 aatcgtagcg ttattgctcg gcattgaagc ttatgcagct gaagaaacct ttgataccca
     6121 ttttatgata ggtggaatga aagaccagca ggttgcaaat attcgtcttg atgataatca
     6181 acccttaccg gggcagtatg acatcgatat ttatgtcaat aagcaatggc gcgggaaata
     6241 tgagattatt gttaaagaca acccgcaaga aacatgttta tcaagagaag ttatcaagcg
     6301 gttaggcatt aatagcgata acttcgccag cggtaagcaa tgtttaacat ttgagcaact
     6361 tgttcagggt gggagctata gctgggatat cggggttttt cgtctcgatt tcagtgtccc
     6421 gcaggcttgg gtggaagaac tggaaagtgg ctatgttcca ccggaaaact gggagcgggg
     6481 tattaatgcg ttttatactt cttattatgt gagtcagtat tacagcgact ataaagcgtc
     6541 gggtaacaac aagagtacat atgtacgttt taacagcggg ttaaatttac tggagtggca
     6601 actgcattct gatgccagtt tcagtaaaac aaataacaat ccaggggtgt ggaaaagcaa
     6661 taccctgtat ctggaacgtg gatttgccca atttctcggc acgcttcgcg tgggtgatat
     6721 gtacacatca agcgatattt ttgattctgt tcgcttcagc ggtgtgcggt tgtttcgtga
     6781 tatgcagatg ttgcctaact cgaaacaaaa ttttacgcca cgggtgcagg ggattgctca
     6841 gagtaacgcg ctggtaacta ttgaacagaa tggttttgtg gtttatcaga aagaggttcc
     6901 tcctggcccg tttgcgatta cagatttgca gttggccggt ggtggagcag atcttgatgt
     6961 tagcgtgaaa gaggcggacg gctcggtaac cacctatctg gtgccttatg cagcggtgcc
     7021 aaatatgctg caacccggcg tgtcgaaata tgattttgcg gcgggccgta gccatattga
     7081 aggggcgagc aagcaaagtg attttgtcca ggcaggttat cagtatggtt ttaataactt
     7141 attgacgctg tatggcggca cgatggttgc taataattac tatgcgttta ccctctgaac
     7201 gggttggaac acgcgcattg gcgcaatttc cgtcgatgcc acgaagtcgc atagtaaaca
     7261 agacaacggc gatgtgtttg acgggcaaag ttatcaaatt gcctacaaca aatttgtgag
     7321 ccaaacgtcg acgcgttttg gtctggcggc ctggcgttat tcgtcgcgtg attaccggac
     7381 atttaacgat cacgtttggg caaacaataa agataattat cgccgtgatg aaaacgatat
     7441 ctatgacatt gccgattatt accagaacga ttttggccgt aaaaatagct tttctgccaa
     7501 tatgagccag tcattgccag aaggctgggg ttctgtgtcc ttaagtacgt tatggcgaga
     7561 ttactggggg cgtagcggca gcagtaagga ttatcagttg agttattcca ataactggcg
     7621 gcggataagc tataccctcg cggcaagcca ggcgtatggc gagaatcatc atgaagagaa
     7681 acgttttaat atttttatat caattccctg tgattggggt gatgacgtta cgacgcctcg
     7741 tcggcaaata tatatgtcta actcaacgac gtttgatgat cagggttttg cctcaaataa
     7801 tacgggatta tcaggaacag tagggagtcg ggatcagttc aattatggtg tcaacctgag
     7861 tcatcaacat cagggaaatg aaacgacagc tggggcgaat ttgacctgga acgcgccggt
     7921 tgcgacagtg aatggcagtt atagtcagtc gagtacttat cgacagactg gagccagtgt
     7981 ttcagggggc attgtcgcct ggtcgggtgg cgttaatctg gcgaaccgtc tttccgaaac
     8041 gtttgctgtg atgaatgcgc caggaattaa agatgcttat gtcaatgggc aaaaatatcg
     8101 cacaacaaac cgtaatggag tggtggtata cgacggaatg acaccttatc gggaaaatca
     8161 cctgatgctg gatgtgtcgc aaagcgatag cgaagcagaa ttacgtggca accggaaaat
     8221 tgccgcccct tatcgcggcg cggttgtact ggttaatttt gataccgatc agcgcaagcc
     8281 ctggtttata aaagcgttaa gagcggatgg tcaaccatta acgtttggtt atgaagtcaa
     8341 tgatatccat ggtcataata ttggcgttgt cggccaggga agccagttat ttattcgcac
     8401 caatgaaata ccgccatcgg ttaatgtagc aattgataag caacaaggac tttcatgcac
     8461 aatcaccttc ggtaaagaga ttgatgaaag tagaaattat atttgccagt aatagtcagg
     8521 tggttctatg gaaattcgca taatgctatt tatattaatg atgatggtta tgcctgtgag
     8581 ctatgcggca tgttatagtg agttatctgt tcagcacaat ttggttgttc aggggaattt
     8641 tgcacttact caaacacaaa tagcgacata tgagcataat tttaatgatt cgtcatgcgt
     8701 gagtacaaat actatcaccc ctatgagccc atcggatatt attgttggac tttataacga
     8761 taccattaaa ttaaatttac attttgaatg gaccaataaa aacaacatca cgttgtcaaa
     8821 taatcagagc agtttcacca gtggttattc ggtaacggtg acacctgcgg ccagtaatgc
     8881 aaaagtgaat atttctgcgg ggggcggtgg ttcagtgatg attaatggtg ttgcgacatt
     8941 atccagtgct tcatcatcga cacgcgggag tgccgcagta caatttctac tgtgtttatt
     9001 aggtggcaag tcatggggtg catgtgtaaa tagctacaga aatgcattgg cacaaaatgc
     9061 aggtgtctat tccttcaatc tgacattgtc atacaacccg ataaccacaa cctgcaaaca
     9121 ggacgattta ttaattactt tagacagtat tcccgtttca caattaccag ccacaggtaa
     9181 caaagcaaca ataaatagta aaaaaaggga tattattctg cgttgtaaaa atttattagg
     9241 tcaacaaaat caaacatcac ggaaaatgca ggtgtattta tcaagttctg acttgttaac
     9301 caacagcaac acaatactga aaggtgcgga agataatgcg taggatttat tcttgaaagt
     9361 aatggttcgc cagtcacact tttaaatatc actaacagca gtaaaggata cacaaattta
     9421 aaagaaattg cggcaaagtc aaaacttaca gatacaacgg tttcaattcc gataacagcc
     9481 agttactacg tctacgatac aaacaaagtt aaatctggcg cactggaggc aaccgcatta
     9541 atcaacgtga aatacgacta ataacaagca aaacgagcaa gtggctaata ataaaaaata
     9601 acaaggtgtc tgcatttccc cctcatgatg agggggcttt ttttagcgat gataaaaaat
     9661 ctcaccgtcg taggctttaa tgattttacc gtcggtgtcg ctgatcaaca cgtacgcgcc
     9721 acccatatag gtccagtgcg tcccggcatc aggcgcgggc aggttacgta actgccactg
     9781 cttaatgttg tactcatcgg tgcggtacat ctctgggacg gtatcacctg gtttgaaatg
     9841 cttaaagtca gcaatgaaac tacttaactc atacttagaa attcccgagg tattggtcga
     9901 acccgcggtg gctggtgcgg cccaggcggc actggtaacc agcaaaagca cacccagcaa
     9961 catcttattt ttaatggtca taaactctcc actttgggga tctgtaatat cagtcgcata
    10021 tctttcctgt catacaatat aactgacaag cgatattttg ttagtgatcc catgatttcg
    10081 taaaaagtgt aaatgaaagg atgccctaaa agcatcctta gcgagcagag agtcagcgga
    10141 aatagctgat ttaagaatca tcctgggctg aagataacat gcaagcctaa aggattgagt
    10201 tatcgcatta tgcccatgaa gccatgtacg cccatataca caccgactaa gccaatcaac
    10261 agactggaaa aatagggggc gcgtttagcg agagtgttaa atccgctcca gcgttttgcg
    10321 acctgctgaa cgctgattgc tgcgccaacg cctacggtga caagcgttaa cgccaggcca
    10381 atgctgaaac tgacgaccag tgttgcgccc agtgtcaggg ctttcaactg aatgcatatc
    10441 aacagcacgg taattgctgc cgggcagggg ataaagccac cggttaagcc aaataacaaa
    10501 atttgccagt tgatgacctc tctaccatca aagcgtcgtt taatgtcatt ggcatgggct
    10561 cgtgcatggg catcctgata ctcgccatat tcgtgatggt gatgatgtcc ttggtcgtgg
    10621 tgatgttcgt ggtcttgatg atgttcgtga tcgtgatgat ggtgatgatg ctcataatca
    10681 tgcccgtgca tattctccag ccagttgcgt tcgccgcgct aggtacgcca gaacatccag
    10741 aacgcggtgc caataatgat cactgcggaa atcagctgga gccacggttc tgctgattga
    10801 gcagtaaagc gcttactgat caccatcccg ccaaaggcaa tcaaccagac caccgcggta
    10861 tgcgaaatgg ttgctgccag tccgagcatc accgcctgtt taatggtgcc tttgatggcg
    10921 atgataaacg ccgccatcat cgtttttgag tgccctggtt ccaggccatg aagcgcacca
    10981 agtaagatgg cgctggggat gaagaaccag gcgtttcctt gctgaagaag agttgtaaat
    11041 tcggtcatga taataattct tagtattaat tcggcaatct gattctactc ccccccagta
    11101 cctgatacta cccccccagt agattaatag tgctatgatt tttcatattc ttgttaacca
    11161 ggtgttgcca tgtctcatac aatccgtgat aaacagaaac tgaaagcgcg tgccagtaag
    11221 attcagggcc aggtcgtggc gctcaagaaa atgctcgacg agccgcacga atgcgctgca
    11281 gttttacaac agattgctgc tatccgtgga gcggtaaacg gtctgatgcg ggaagtgatt
    11341 aaaggtcatc tgacggaaca catcgttcac cagggggatg agctaaaacg tgaagaagat
    11401 ctggatgtcg ttctgaaggt gctggattca tatatcaaat aatttattaa cgcgattgta
    11461 aaactgccgt ttttcctcgt ttacaacggg ggcgctggac attaccatcc tcctctgcga
    11521 tttatcatcg caaccaaacg actcggggtg cccttctgcg tgaaggctga gaaatacccg
    11581 tatcacctga tctggataat gccagcgtag ggaagtcacg gaccaccagg tcattgcttc
    11641 ttcacgttat ggcaggagca aactatgcaa gtcgacctgc tgagttcagc gcaatctgcg
    11701 cacgcgttac acctttttca ccaacattcc cctcttgtgc actgcatgac caatgatgtg
    11761 gtgcaaacct ttaccgccaa taccttgctg gcgctcggtg catcgccagc gatggttatc
    11821 gaaaccgaag aggccagtca gtttgcggct atcgccagtg ccttgttgat taacgttggc
    11881 acactgacgc agccacgcgc acaggcgatg cgtgctgccg ttgagcaagc aaaaagctct
    11941 caaacacgct ggacgcttga tccagtagcg gttggtgcgc tcgattatcg ccgccatttt
    12001 tgtcatgaac ttttatcttt taaaccggca gcgatacgtg gtaatgcttc ggaaatcatg
    12061 gcattagctg gcattgctaa tggcggacgg ggagtggata ccactgacgc cgcagctaac
    12121 gcgatacccg ctgcacaaac actggcacgg gaaactggcg caatcgtcgt ggtcactggc
    12181 gaggtggatt atgttaccga tggacatcgt atcattggta ttcacggtgg cgatccgtta
    12241 atgaccaaag tggtaggaac tggctgtgca ttatcggcgg ttgtcgctgc ctgctgtgcg
    12301 ttaccaggcg atacgctgga aaatgtcgca tctgcctgtc actggatgaa acaagccgga
    12361 gaacgcgcag tcgccagaag cgaggggcca ggcagttttg ttccacattt ccttgatgcg
    12421 ctctggcaat tgacgcagga ggtgcaggca tgaaacgaat taacgctctg acgattgccg
    12481 gtactgatcc gagtggtggt gcggggattc aggccgatct taaaaccttc tcggcacttg
    12541 gcgcttatgg ttgctcagtt attactgcac tggtggcgca aaatacccgt ggcgtacagt
    12601 cggtgtatcg cattgagcct gattttgtcg ccgcccagct cgattcggtg ttcagcgatg
    12661 tgcgaatcga taccactaaa atcggtatgt tggcggaaac cgatattgtt gaagcggtgg
    12721 cagaacggtt gcaacgttat cagatccaaa acgtggtact cgacaccgtt atgctggcaa
    12781 aaagcggcga cccgctgctt tcaccttcgg cggttgctac gctgcgcagt cgattattgc
    12841 cacaggtttc attaataacg ccaaacttgc ccgaagctgc cgccttgctc gacgcgccac
    12901 acgcgcgcac cgaacaggaa atgctggaac aagggcgatc gctgttggcg atgggctgtg
    12961 gcgcagtgct aatgaaaggt ggtcatctgg atgatgagca aagcccggac tggctgttta
    13021 cccgcgaggg tgaacaacgg tttaccgcac cgcgcattat gaccaaaaac acccacggca
    13081 ctggttgtac actctctgcg gcgttggctg cactacgccc gcgccataca aactgggctg
    13141 acaccgtaca ggaggcaaaa atctggcttt catcggcgtt agcccaggcc gacacgctgg
    13201 aagttggtca cggtattggt ccggttcacc acttccacgc ctggtggtga gcaccgtact
    13261 atagctgctt tggttattgt cagcctttgg cactgacatt cagggatgaa accaatgcag
    13321 ctaagaatta ccagtcgaaa aaaactcacc gctctattat gcgctcttgg gctaatttcc
    13381 atcgttgcaa tttatccgcg tcagacggtg aattttttct attcgacagc agttcagatt
    13441 acagactaca tccacttcta cggttatcgc ccggttaaat ctttcgctat tcgcattcct
    13501 gccagttaca ccattcacgg attagatgtt tcgcgctggc aggagcgtat cgactggcag
    13561 cgggtggcaa aaatgcgcga caacgatatc cgcttacagt ttgcttttat taaggctacg
    13621 gaaggcgaaa agctggtgga cccctatttt tcgcgtaact ggcaattaag ccgcgaaaat
    13681 ggcctgctgc gcggggcgta tcattatttc tccccgtcgg tatccgcttc agttcaggcg
    13741 cgattatttc tgcaaacggt ggatttctca caaggcgatt tccctgccgt gctggacgta
    13801 gacgaacggg gaaaattatc ggcaaaagaa ttacgcaagc gggtaagtca gtggctaaaa
    13861 atggtcgaaa aacgcacggg aaaaaagccg attatttact caggagccgt tttttatcac
    13921 accaatctgg cgggctattt caatgaatat
//
LOCUS       DAEEUE010000100        13822 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.100, whole genome shotgun sequence.
ACCESSION   DAEEUE010000100 DAEEUE010000000
VERSION     DAEEUE010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13822
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.100"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            409..1239
                     /locus_tag="KLK84_003226"
     CDS             409..1239
                     /locus_tag="KLK84_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HBE2010450.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="KLK84_003227"
     CDS             complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="KLK84_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HBE2010451.1"
                     /translation="MKSVTSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2368..3828)
                     /locus_tag="KLK84_003228"
     CDS             complement(2368..3828)
                     /locus_tag="KLK84_003228"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HBE2010452.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="KLK84_003229"
     CDS             complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="KLK84_003229"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBE2010453.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5433..6776
                     /gene="gntP"
                     /locus_tag="KLK84_003230"
     CDS             5433..6776
                     /gene="gntP"
                     /locus_tag="KLK84_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HBE2010454.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="KLK84_003231"
     CDS             complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="KLK84_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HBE2010455.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="KLK84_003232"
     CDS             complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="KLK84_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HBE2010456.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="KLK84_003233"
     CDS             complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="KLK84_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HBE2010457.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(9005..11641)
                     /gene="fimD"
                     /locus_tag="KLK84_003234"
     CDS             complement(9005..11641)
                     /gene="fimD"
                     /locus_tag="KLK84_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HBE2010458.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIYGIARGTAQVTIKQNGYGIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMEALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11708..12433)
                     /gene="fimC"
                     /locus_tag="KLK84_003235"
                     /pseudo
     CDS             complement(11708..12433)
                     /gene="fimC"
                     /locus_tag="KLK84_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
     gene            complement(12470..13009)
                     /locus_tag="KLK84_003236"
     CDS             complement(12470..13009)
                     /locus_tag="KLK84_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBE2010459.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(13074..13622)
                     /gene="fimA"
                     /locus_tag="KLK84_003237"
     CDS             complement(13074..13622)
                     /gene="fimA"
                     /locus_tag="KLK84_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HBE2010460.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT         3404 a         3533 c         3285 g         3600 t
ORIGIN      
        1 acgtagatga aaatacttgt caccgctgct acttaattcc ggtgggaaat tatttatttc
       61 cgcataatca gctatgtatt atttgaaaca attagtttgt aaataatata aattgataac
      121 atatcaaata catcaaaaat accattcaca ggcattatta atatagacct caatatagca
      181 atatcaaatt ccaattagta ataagatatg cttttaaatt gcatgcaaat aattttaaat
      241 gagcgttttt tgatacttta gaatgtttcc taccctttaa tatcactgtg tagagttgat
      301 atcactacaa cagaaacatg catgtcattt gtttacttct ctttttacta cagaaaaatt
      361 tatctatttt tctaaacatc agttaaatac tcataaaagg taactattat gtctatgcca
      421 ttaagcaatg ccgctaataa ccaattcaat acaaataatc acttccttca tcatccaaaa
      481 gtcgacagtg agttaacacg tcgatatgaa tatgcacgcc tggatacgga aaatatctat
      541 cttctgcctc ttgctcgtgg caataaccat aattacgatg gaaaaagcgt tgtcgaaatt
      601 agaaaactta atattggcaa acagtcctgg ccatttaatt atgtgacaga tacatgcaga
      661 gaattcaatg gtattacaac aacaggacgt atgctctatc gaaacttgaa aatcacctca
      721 gctttagacg aaatatatgg tggtatatgt aaaaaggcgc atgctgctac agagttagca
      781 gaaggtttac ggctaaattt atttatgaag agccctttcg atcctgttga ggactataca
      841 gttcatgaaa taacattagg ccctggttgc aatgttcctg gttatgccgg aaccacgata
      901 ggatatattt caacccttcc agcttctcag gcaaaaagat ggaccaatga gcagccaaga
      961 attgatatct atattgatca gataataact gtctcagggg tagcaaattc ttccggattc
     1021 gcccttgccg cattacttaa tgctaatatt gagatgggaa atgacccaat cattggtata
     1081 gaagcctatc ctggtaccgc ggagatccat tcaaaaatgg gatataacgt tatccccggt
     1141 gatgaagatg cgccactcaa acgcatgact ctgcaaccgt catctcttcc agaactgttt
     1201 gaattaaaaa atggtgaatg gaattatatt ggtaaatagc cactgagaag aatgagtact
     1261 aatattcagg ttatacagat atttaaacaa aaccctgcaa tttacaaagt aatactcatt
     1321 gcgacttctt aatttaagaa agcgcaagga acgtttaccc ttgcgcttat tataataagt
     1381 caggcgtcga ctttatccag cggccatgaa tcaaagagat agccgtcaaa ataaatatcg
     1441 tcaacgttcg agaattccag cagacgttgc ttaacgtttt ccagatgctg ccacattgcc
     1501 agcttcgccg ctcgggcatc ttttttgatt aacgcggcga ggatctgttt gtgatcgccc
     1561 aaccactctt tgcgatacag gctgtcatcc agatggctgt gcaactggat ccacattgga
     1621 ttgttttccc gccactgcca ggactgacgg aataactcca ccagcatgct gttatgcgtt
     1681 gcttcagcaa tggcgagatg gaactgcatg tcaccgcttt cgctgctacc cggcgcgctg
     1741 gaagccagtt cacgctcttc cagttgcaat gcctgacgca ttttgacgat atcttcgcgg
     1801 gtagcctgca gtgcggcaaa ctcggcgatg ttactttcca gtaactgacg cgcctgtaac
     1861 agctcaaaag gacccgcatc attgcagacg ttggcatccg gactgtctgt gttgtggctg
     1921 cctgagctat caagcacata gataccggca ccccggcgca cttccaccag ccctttgatc
     1981 tccagcatga tcagcgcttc acgtaccacc gtccgcgtga catcaagcat ttctgcaatt
     2041 tcacgctccg gcggcagccg ttcgccagga ttgtacggcg tctttatgat cagatcgcgg
     2101 atcatcgccc cgacttcctg gtaaggtctt tgcgcagagg tgacagattt catggtgttg
     2161 tgtccgttag gtgcggttaa tcaaattttt gccggatgcg gcgtgaacgc cttatccggc
     2221 ctacaacggg caattttacg gcccccgtta atctcaaact ataccattag cactggaagt
     2281 tggcacgtac aggtgggcaa aaaaacgaaa actggtcaac caaatatcag tttgattgac
     2341 cagtaagtct gtagggtaat cagttagtta cagcgcagcc acacactcgc gcgcaccgcg
     2401 ttcgcacagc tgctgatatg ccgccgtcac cgcgccaaca aagtcggcat tctgcggcag
     2461 atcatcggca aaaataccgc tcaggcccag cagcgctttc acgcggtctg cgccctgata
     2521 ctgcgcgtta atcttctgga actcggccag catcgggtcg accacgtcaa tggcattacc
     2581 ctgctcatcc acgccctggg tgtaacccat ccagccagcc acgcccagcg ccaggtgacg
     2641 ccagctgccg ccgttttgca ggtgcagacg caccgggtcc agtagacgct gcggtaactt
     2701 ctggctgccg tccatcgcaa tctgccaggt acggtgacgc agagacgggt tgctgaaacg
     2761 ctcgatcagc agcgtcgcat aggcgttcag gtccgtacct tccggcatcg acagcgttgg
     2821 cgcttgttcc tgcatcatca gggcaaaggc cgctttgcga taatccgggt tagtcatggt
     2881 gtcggcaatg gtttcatagc cgccgaggta accgaggtac gccaggaaag agtggctgcc
     2941 gttcagcata cgcagcttca tcatttcgaa cggcacaacg tctgcaacga actgtgtgcc
     3001 cactttatcc cagtccgggc gaccattaac gaagttatct tcaatcaccc actgacggaa
     3061 cggttcgcag gcaatggcgc acgggtcgta aacacccagc tggtcagcaa tttcctgtaa
     3121 ggtttctggc gtcgccgccg gaacgatgcg gtcaaccatg gtgcacggga aggtgacgtt
     3181 ctcttcaatc catgccgcta gctgcgggtc acgcgcctga gccagcccca gtaccgcgac
     3241 cttcgccaca tggccgtttt cacgcacgtt atcgcaggac atcaccgtaa atgctttcag
     3301 ccctttttca cgacgcagac gcaaggcttc gacgatgtaa ccaatcgcgg acttcggcgc
     3361 agtcgggttt tccagatcgt gcttgatcag cgggttattg agatccagtt gaccgcttgc
     3421 cgcatcagcg cagtagcctt tttccgtgac cgttagagag acaatcgccg tttgcggacg
     3481 cgccatcgcg ttgagaatac cttcgcagcc gtcgatttcc ggatgcagcg cttctttcat
     3541 cgaaccgata attttcagct cggtgctttc tgcgcctttt tccgctacgg tgtacagcag
     3601 ttgctgtttt ttcaggtttt cgatcagcat gcggtcgttg cctggcatca ggttaacttc
     3661 gcagatgccc cagtcgctgt cggtgctttc cagcagatga tgggtataca gcgcctggtg
     3721 cgcgcggtga aacgccccgc aaccgagatg cacaatgcgt gattccagac gagaatgatc
     3781 ccatgacggg cgggcaaccg gcagattgct gtcaacaata gtagtcatcg taaactccag
     3841 aatccgtctt cagagagtga cggcaaggaa gaaggaaccg gcacggcgcg tagccatgcc
     3901 ggtgggtatt aacggctaaa gaaagcgcgc tggatcgcca gttcgacacc gcgaacttcg
     3961 gccaggcctt tcagacgacc aattgcggag taaccagggt tggttttctt cttcaggtcg
     4021 tccagcatct gatgaccgtg gtccggacgc atcgggatca ggtcttcttt gccttccgct
     4081 ttacgacggt gttcttcttc aacaatcgct ttcaccactt cgtacatatc aacgtcaccg
     4141 ttcaggtgcg ccgcttcgtg gaaggttttc gggttatctt cacgcatggt ggagcgcaga
     4201 tgggtgaagt aaatacgcgg accgaactgc ttgatcatat caaccagatc gttgtcagca
     4261 cgcacgccgt aggaaccggt gcacatggtg aaaccgttcg ccatgctgtt tacggtatca
     4321 accatccact gcatatcttc aatggtggaa acaatgcgcg gcaagccgag gatcgggcgc
     4381 ggcggatcgt ccgggtgaac cgccatacgc acgccaactt cttcagcaac tggaataatc
     4441 gctttcagga agacggcaaa gttttcgcgc agtttggctt tgtcgatatc tttgtacagc
     4501 tccaggtgct tacggaactg gtcgagggta tacccttctt ccgcgcccgg cagaccggca
     4561 atgatgttac gcgtcagacg cgctttgtct tcgtcgctca tagtggcgaa gcgttcagct
     4621 gcctgagcaa tttcttcttc ggtgtaatcc gcttccgcgc ctggacgttt caggatatgc
     4681 atttcgaatg cagcgaattc gatctggtcg aagcgcagag ctttggagcc gtctggcagc
     4741 acgtattcga ggtcagtacg ggtccagtcg agcaccggca tgaagttgta gcacacggtg
     4801 cgaatgccac actgcgccag gttgcgcaga gtctgctgat agttagcgat ccactgctca
     4861 tagttgccag tgtgggtttt gatatcttcg tgaattggta cgctttctac gacagaccac
     4921 accaggcctg cgtcttcaac gatcgccttg cgtttgagga tctcttctac ggaccatact
     4981 tcgccgttcg ggatatggtg cagcgcggta accacgccag ttgcgcccgc ctgacggaca
     5041 tcagctaaag aaaccggatc gtttgggccg taccagcgcc aggtctgttc catgattttt
     5101 cctctttcac atagtgggtt ccacagcaaa gagacgcgga accttggagt tgtttaggta
     5161 tccggatgaa caaccagacc gcagccgtgg cgttgcatcg gatgagatgg cgtataagtt
     5221 ctaccttttg gtaagacatg tcaaccagaa ttctgaaatt ggttaactgc atcgcaagaa
     5281 tttcactctc atggttgttg ctgcaaattg cagcaaaggg taatcgatgg ttattgatca
     5341 gaggatgtga cattcatcgc aacaatggtt gaccaattta cataacatgt cgcccaaaat
     5401 aacaacggtt caaccaagta aggtaaaacg ttatgcatgt gcttaacatt ctctgggtgg
     5461 tattcggcat tggcctgatg ctgatactga atttgaagtt caaaatcaat tcaatggtgg
     5521 ctttgttggt ggcggcgctg tccgtcggga tgctggcagg catggatttg atgtcgctgc
     5581 tgcacaccat gaaagcgggc ttcggcaaca cgctgggaga actggctatc atcgtggtgt
     5641 tcggtgcggt catcggtaaa ttgatggtcg actccggcgc ggctcaccag atagcacata
     5701 cgctgctggc gcgtctcggt ctgcgctatg tacagctgtc ggtgattatc atcggcctta
     5761 ttttcggtct ggcgatgttc tatgaagtgg cctttatcat gttagcgccg ctggttattg
     5821 ttattgccgc cgaagctaaa attccgttcc tgaaactggc gatcccggca gtagcagctg
     5881 ccactaccgc acattcactg ttcccaccgc agccgggtcc ggtggcgctg gtgaatgctt
     5941 atggcgcgga tatggggatg gtttatatct atggcgtact ggtgacgatc ccaagtgtaa
     6001 tctgcgcagg tctgatcctg ccgaagttcc tcggcaatct tgagcgccca acgccatcat
     6061 tcctgaaagc agatcaaccg gtagatatga ataatctgcc ctctttcggc gtttcgattc
     6121 tggtgccgct gatcccggcg atcattatga tctctaccac catcgccaat atctggctgg
     6181 taaaagatac ccctgcctgg gaagtggtta actttattgg ttcctcgccg attgcgatgt
     6241 ttattgcgat ggtggttgca ttcgtactct ttggcaccgc gcgtggtcat gacatgcagt
     6301 gggtgatgaa cgcttttgaa agcgcggtga agagtattgc aatggtgatt ctgatcatcg
     6361 gtgcgggtgg cgtgctgaag cagaccatca tcgacaccgg cattggcgac accatcggca
     6421 tgttgatgtc ccacggcaat atctcgccct acattatggc atggctgatc actgtgctaa
     6481 ttcgtctggc gacgggtcag ggtgtcgttt cggcgatgac cgccgccggg attatcagtg
     6541 ctgcaatcct tgatccagca accggtcagc tggttggcgt gaatccggcg ctgctggtac
     6601 tggcgacggc tgcgggttcc aacaccctca cccacattaa tgatgcatct ttctggttgt
     6661 tcaaaggtta ctttgacctg tcagtaaaag acacgttgaa aacctggggt ctgctggagc
     6721 tggtcaactc cgtggttggg ctgattattg tgctgattat tagcatggta gcgtaaaaga
     6781 acaccagagc ctgccaatgg caggctcaga ctgatgacta aagtctaaaa atgtgcccgg
     6841 acagtcccct cgcccctccg gggagagggt tagggtgagg ggaacagacc agtaaagtgc
     6901 gctttgttta tgccagatgc gacgctgacg cgtcttatct ggcctacaaa gggctaacgt
     6961 gcaggttttg taggtctgat agcgtagcgc ctcaggtacc agcattagca atgcgctgta
     7021 atttttttat tgataaacaa aagtcacgcc aataatcgat tgcacattcc ctgcagtcac
     7081 ctgccctccg gtacgtgcgt aatttgccgt taatcccaga cttaccgccg aagttcctac
     7141 tgctcctaac gataccgtgt tattcgctgg aataatcgta ccgttgcgcg tcaactgtac
     7201 gccgacgccc tgcgctggtg aaaacgacgc ggtattggtg aaaatcgagt tgcccgcatc
     7261 tgcggttgtg ccggagaggt aataccccag gttttggctt ttcgcacaat aaacggtaag
     7321 aggaattggc actgaaccag ggtagtccgg cagagtaacg gtgacatcac gagcagaaac
     7381 atcacaaccg ccagtgggca ccaccacatc attattggcg taaatattcc acacaaactg
     7441 aaaatcatcg ctgttatagt tgttggtctg tcgcaaaata agcacggcaa ttaatgaacc
     7501 agctttaatc gccaccccgc ccgcactgct cacaggcgtc aaataaagcg ccaccggcca
     7561 cggcttatcc gttctcgaat tataaacaac ccgcggcgtt tcgctggtgg tcggaaatgg
     7621 atagctactg ccactatatt ttacggtccc ggaaaaatta gataacacgc cgccgtaagc
     7681 cgagcctcgt tgcagtgtga catagtctgt aatggtttcc ggataatcgt tatggcaaaa
     7741 gatttgcgtc gaaagatcta cgaccaggtt ttgccccaca ttcacgacag gcgcaaggtt
     7801 tacataaaca ttagcgctgc caccgccaat agggatagcg gtaccattgg cggttttaca
     7861 ggcgaatgac caggcattta ccgaccagcc catcagcagt acagcaaaca gggtaatagc
     7921 tcgtttcatt acaattatct cttcgggttc agctgtaggt ataggtgatg ctaatcactg
     7981 cctgaatggt tccctgagtg gctccgccat ttaccgtcaa tgctctgacc tgtaacggga
     8041 agtgcgctga ttgtgaggaa tcatccacct gaactgtttt ggttgcgcca gtattcaatg
     8101 tgttgccact gtcatcctgt agctctaact ggatgttttg cgcggtcccc tggtttttat
     8161 aatatccggt gctgtcggct gccccgctga agctggcagt gaccctcgac gttcccaccg
     8221 gacaattagt caactcaagc gcaacatcat gccaggccga tgccgccccg gcagacataa
     8281 gactgaaaga ataaagatcg ccgagatcta ccgtggcatt ggtggtggaa accgtacacg
     8341 gtttggcgac gaccttaccg ttcaccgtga tggtgacatc ggctgcctgt atcgtcgcac
     8401 ttgcgagcgc caacattgcc gccaatacat acccacgttt gcaccatttc atgagcatct
     8461 ccagttactg atattcaaga gtgaaggtag ccgtagcatt gatatgcccc gcagtgacag
     8521 gcacctgtgt cgccattagc cgggcgtaaa aattcagcgt atttggttta cccggcgtca
     8581 gggtcgtcca cgaaatcgca gacgatggag cattaagggg tatttgattt tgctgctcat
     8641 tcagaagctg tattcccagt cccgcagccg ctgacaccgt attttcaagt gcaagcaggt
     8701 tggcattgtg gctatctgca acgccggtaa acccaacttt tacggcagaa acggcattac
     8761 cacagggtga cagcaaaata cgaaatggaa cgacaggagt cgtcgcgcca atgttgttaa
     8821 attgcttcgc cgcgttttcc atcagatcaa cagtaaaatt ggttgattca gcggccacac
     8881 tacagccgtt atctctgaca tagccgcgga tagtaatcgt gctatccgca gccaaaacgc
     8941 gacttaccgc cagccacaaa aaagcgcaca gaagataaaa aggtttgttt ctcatcacgc
     9001 ccccttaacg acattcagct gatagctggg ttaataactg ctgctgactc tctggtggca
     9061 gttgataatt ggcgacacag tgagcatttt cctcttctcc ccatttcacc tgaacttttc
     9121 ccgctaaagg cattccgctg aggtaaacct gaccattatc cgcaacaatg ccgctactct
     9181 ggctactctc tgatgtcacc atcgccccaa acggcagcgg cttattattg tgagttagcg
     9241 tcatgagcag ttttatccca acgcgcgctt taaactctgc tcgcacgatc gccccacgag
     9301 tgggaacaac gttagcgacc gcgttatcta aatcgacgtt atcagccaag gtattggtat
     9361 ccagcgccac tctattttcc cgatattcag tggcataagg cagcacggca taaccacgcc
     9421 agtcggtacg cacccccgtc tggttttcga cttttgcatc ttttgcgcca ggcgctttaa
     9481 caagcaccac cgtatcgttt aacggctgcc ccagcgttac gccattggca tgagccaata
     9541 ccccaccgct gactccgtaa tagagctgct taatatcatc gctatggctg taaccgatat
     9601 tggcattgcc gtaaccaccg cgataattca gcgtggcgta gcctgtactt ccgctattac
     9661 catcgcctcc cccggcatag ccggtttgca cgctataact gaggttgttg tcttccagca
     9721 acgtaccgta tacaccagcc agattggtca tccgaccgtt gagatcgtgt gacatgctgt
     9781 agctggcact ggcatgtcgc cactgagatt tactgtcaga acgcagccag tggctgaaag
     9841 gaatattgac gttaagcgct aacatctgat cccgtccttt ttgccaggcg tttttcgtca
     9901 ggctatagct gagcgtccag ttgatatctt cgaacgcagt atttaatcca gcctggaatt
     9961 gctcatcgac attactcgtt ccccaataag tttgatggct accactcaaa tacagtgttg
    10021 atgtgcgccc gagttgctga gtaacggtga gctgtaattt cccgcgtttg ttataagcga
    10081 ggttgtaata gtcggtgaat ttcggcttaa cctgaataac tccatcctgt gtttcgatgt
    10141 tgtagccatt cattcgactg tatgttgtat cagcgaaatt aaaatatccg ctggtcgaat
    10201 aacggtaacc cactaactga atattcgtgc ctgattcatt gagcgatttg ttatagagaa
    10261 aacgcaccga ttgtccgtca tgctgactgt catcgggaag tgtggaatta gcctgcgtca
    10321 tatccacaga cagagcgccc agtgcctcca tgtttttccc gataccgaaa ttaaaagcac
    10381 gataacgatc cgccagttgc gttccaccat atattgtcca gccagccgga aggccgtgga
    10441 gtaatgtact ctggaaaaag cggggttttt cctgctgcgc atttccacta cggtattctc
    10501 ctgccgtaat ggaataacga gtatgccctt cacgttgcaa aagcgggact gacgaatagg
    10561 gtacggtaaa aatctgcgtg ctgccgtcag cctctttgat cgttacctgc aagtcaccac
    10621 tattacctgc ggcatagata tcgttgatgg taaaaggccc cggtggcacc gtactattat
    10681 aaatgccata tccattttgt ttaatagtga cctgtgcagt accacgagca ataccgtaga
    10741 tcaccggggc aaatcctctt tggctatcgg gtaacatatt gtcatctgag gccaattgtg
    10801 cgccgcgaaa gttaatacca tcgaaaatat cgccctgggt ataaccatca cccaacgtca
    10861 gccgggaacg taaaggaatt atgtctcgct caatccaggt aatgatatgc tgccatttat
    10921 ttttgctacc tgatgaactg tcgctactgt tataactcca ggtggtattg tcgcgtaaac
    10981 gccacgcacc aatatttaac ccactctgta ggtttaaata tgcataatgg ctgttacccc
    11041 caatccgatt ctgtacacta tttccgctga aattataatt gagcaatccg gcattaatac
    11101 cgggatccca taactcagga ggaatataac cacgcgcgcg attactcata aatgcctgag
    11161 ggatcgtcag gttcagtcgc tgctgaccaa catccagatg cgcagtagcg tcttggacca
    11221 ttgtggttaa tggcacacag gcatcatccg ccagcagatt cataccggcg acagaagccg
    11281 tattcagccc catactggcg agttgcgcgc gtgtcaggca gggaacaatc ccttgttcac
    11341 tgtcgcccgt attaaatgtg acatcacgcg ttgccatata accattattc aaatagatat
    11401 cgacgcgata cgtccctggc ggtaattctt gcccattttc aaaacgcgat aaatcggcca
    11461 cagcctgggg atcatccgct aaaaagcgca gattaaaata gaggtcggca gatgacaaag
    11521 gtgcctgtgc ggcaaaagca caggcgacaa cgagtcggac aaaaaaacca gccaaacgat
    11581 gcttacgaat atgcaagcat tgtgtgtttc gctggtaaag tcttaaattc agatatgaca
    11641 ttattcctcc ggtttaaaat cacccggcag tcaattcttt ttattcaggc gaaaaattcc
    11701 ccctgcgtta ttccattacg cccgtcattt tgggggtaag tgcgccataa tcatttattg
    11761 ttcggtaagt aatattgctt cctgcatcag aaggcaattt aaccgtgctt tcgcccattg
    11821 gaggcaccaa tgcattttca agaacccggg ttccggcatt caactctgtt accgtcaggt
    11881 aatagggtgt cgggttaatc agcgtcagag aattcgcgct acgacgaaat cttaattttt
    11941 ctgcggcctg atcgggtggc aacgctaatt tagccgggcg atagtacagt ttaatgcggc
    12001 tgataattgc gagctgtagc atattctcag tcaattttga tttatccatt gacggaatcg
    12061 ctttaacgtt catccagaat aaactttccc ggtcctgtgg caattggtta tttgttgcat
    12121 caagaatacg taaggtattc tctttttttc ccttcatcgc aaacagagga ggcgtcacga
    12181 taaaacgacc atcctttaca ccatcggcat tttccaccca tgattgaatt aaataggtac
    12241 tattttcatc attatttgtc acggcaagtt gcacttgttt ttaccctgcc ggataaatta
    12301 cgcgagtcgc acctaaggcc actcctgctt cagcgcgtcc ggcaaccatc attgccatga
    12361 acatcaggat acctgccagc aagcagaatg ttatttcctg cgatttcctt acattgacgt
    12421 ttttattact cactgtcctg ttcctgttat cacattatct actgaacaat tactgatagg
    12481 ttaaagagaa ccaggcctgg gcattggcga tgccgccagt aacccgacgc ccggtagcac
    12541 gatatttggc gatgaaatgt agcgaagtag agcctgagta aagccgtttc cagtttgctg
    12601 gaggacgatt aatcggtacg aggtttcctt catcatcaaa caacgctaca ccaatattgg
    12661 aggctatccc tggcccctct cccacggaga gcacatccgg atttttacca tccgcgacac
    12721 cgtgaaacgc cacacccaca cgttcactca ccaccgtgct acattcccgt aaatgaataa
    12781 caaaaggcac cggtgcgcta tcttcccctg ccgcatgaaa ccggttactg ctgatttgcc
    12841 ccatattgac cgtcatttgt ttatcaccgg cttcaatccg gcaagtttcc gcaataatga
    12901 cgccctgaaa ttgcatattt ccgccgggca gcgtggtatt ccatttattt ccggccagag
    12961 caaacattgg cagcaacgac gctaacagaa ataatctctt ccttttcatc gttattttta
    13021 tcgcacaagg gtgggcatcc ctgcccgtaa tgacgtcctt gaacctgggt aggttattga
    13081 tactgaacct tgaaggttgc atccgcatta gctgcacccg gggttgcctc gccgattgca
    13141 taataacgcg cctggaacgg aatggtgttg gtaccgttat tcagggttgt ttgtgcacta
    13201 aatgtcgcac cgtccagcgt cagagcattg cctgttctgt ccaggatctg cacgccaacg
    13261 tttgttgcac tacctgcagc agaactctgc agagccagta catcagtacg cgtagcatca
    13321 attgccgtac ctaagaaggc aacagcggct tttgtggcaa cagtggtatc gcaatcattc
    13381 agctgaatgt taaaaccaac ggcagagctg gttgctccag cctgcttcag gctagcggta
    13441 cgaacctgtc ccaactgaac ggtttgatca acagagcctg catcaactgc gcaagcggcg
    13501 ttaacaactt cccctttaaa gtgaatggtc ccaccattta ccgtcgtagt atcggccaga
    13561 gccgctgcgg aactgaggga cagagccgac agaacaacga ttgccagagt tttaattttc
    13621 atgctgcttt cctttaaaaa aactatttct aaatcgacat gggcagtcgt tctgtacact
    13681 ttgttttgtc aacgagtttg actgccaaca ctgcacagtt ttcccccaaa agatgaaaca
    13741 tttggggcca ttttgactca tagaggaaag catcgcgcac aaactttttc agtttatttg
    13801 ttggcttaat attctattat ta
//
LOCUS       DAEEUE010000101        13800 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.101, whole genome shotgun sequence.
ACCESSION   DAEEUE010000101 DAEEUE010000000
VERSION     DAEEUE010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13800
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.101"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..818)
                     /gene="wcaD"
                     /locus_tag="KLK84_003238"
     CDS             complement(<1..818)
                     /gene="wcaD"
                     /locus_tag="KLK84_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HBE2010461.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLGT"
     gene            complement(793..2010)
                     /gene="wcaC"
                     /locus_tag="KLK84_003239"
     CDS             complement(793..2010)
                     /gene="wcaC"
                     /locus_tag="KLK84_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HBE2010462.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREILALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2007..2495)
                     /gene="wcaB"
                     /locus_tag="KLK84_003240"
     CDS             complement(2007..2495)
                     /gene="wcaB"
                     /locus_tag="KLK84_003240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HBE2010463.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2498..3337)
                     /gene="wcaA"
                     /locus_tag="KLK84_003241"
     CDS             complement(2498..3337)
                     /gene="wcaA"
                     /locus_tag="KLK84_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HBE2010464.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(3430..5592)
                     /gene="wzc"
                     /locus_tag="KLK84_003242"
     CDS             complement(3430..5592)
                     /gene="wzc"
                     /locus_tag="KLK84_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HBE2010465.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5595..6038)
                     /gene="wzb"
                     /locus_tag="KLK84_003243"
     CDS             complement(5595..6038)
                     /gene="wzb"
                     /locus_tag="KLK84_003243"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HBE2010466.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6044..7183)
                     /gene="wza"
                     /locus_tag="KLK84_003244"
     CDS             complement(6044..7183)
                     /gene="wza"
                     /locus_tag="KLK84_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HBE2010467.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7492..7641
                     /locus_tag="KLK84_003245"
     CDS             7492..7641
                     /locus_tag="KLK84_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010468.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7842..9425
                     /locus_tag="KLK84_003246"
     CDS             7842..9425
                     /locus_tag="KLK84_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBE2010469.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9877..11730)
                     /gene="asmA"
                     /locus_tag="KLK84_003247"
     CDS             complement(9877..11730)
                     /gene="asmA"
                     /locus_tag="KLK84_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HBE2010470.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11752..12333)
                     /gene="dcd"
                     /locus_tag="KLK84_003248"
     CDS             complement(11752..12333)
                     /gene="dcd"
                     /locus_tag="KLK84_003248"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HBE2010471.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12425..13066)
                     /gene="udk"
                     /locus_tag="KLK84_003249"
     CDS             complement(12425..13066)
                     /gene="udk"
                     /locus_tag="KLK84_003249"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBE2010472.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13384..>13800
                     /locus_tag="KLK84_003250"
     CDS             13384..>13800
                     /locus_tag="KLK84_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HBE2010473.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLVSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPLLG"
BASE COUNT         3193 a         3640 c         3495 g         3472 t
ORIGIN      
        1 gtacctaaat cgcccagacg ggtggaaata taaggaaacg cgattaccac accaacccgg
       61 aatacagcca gagaaatcaa tgctaacggt aattttttct taatcgcctc tttattcaga
      121 tattgaaatg cccactccag caaataaaac aggataaagg tcataacccc tgaaaacgat
      181 ccggataata ttatccctgc gagaatcagg gcatctgttt taggcgtttt gataccaaac
      241 tgtttgatgc tgagccaaat tgagattaat gccagagcga aaaatgccgg ttcgaaataa
      301 agtgctgtcg tgcgcttgcc gccgaattta atgaaattca gcacatagct gttgctgtaa
      361 atcagatatt tcgaaatcga ctccataata ctgctgccac cggtgaggat aatttgcgcc
      421 atctccaccg ccgccagcgc caccaccagt ccgaccacca gataaaagaa acgtaatatc
      481 ttacgatggt tgtgtggcga aattgtttta aaacgaatac tccacaccat gccaataatg
      541 atcacaatat agacaaacag catagttgaa gtgacgtatt taccggcatc caacgactga
      601 ccaaacaggt agttaaacgc cgtgagtccc gcgccaatcc ctaaggcaat catcaatttt
      661 ttaatgctga tgcgttctaa aaacagcagt aacaagacag gtaaaaaagt gacgatggtg
      721 atggggaaac tttcgccaag ctgggcgatt ttgacgttaa ccagcaggta gatcagtggc
      781 agcagcaggt agctacagat tctgatagaa gttgacatac tcctccagca tctgttgtcc
      841 actgtaggcg gcgcgactgc gttggctgaa ctcagccagc gtggtaccaa atatcgcctg
      901 cgcgatttcc ggtttgctta actgtaccag ttgcagcacc tcttcttcac tgacggtttt
      961 accaccggat ttttgcagca cttcccgcgc cgcatcgcta tgggtggcaa tcaccggcac
     1021 gccaatcgat agcgcctcac acaaaatcag cgggtagtta tcgacacgag aactgaacac
     1081 cagcgcatcc atctgattga gcgcgctcat caacttgcgc ttgtcagttt caaagccgtg
     1141 attaaccacg ttgccagcgg tgaacggcga gaacttacca aaggtatgca gttcgatttt
     1201 gtcgcccagc gccatcatct cacgcaccag ttgctggtta gttttgccgt cgtaacgcag
     1261 gtcatgcgcc accactgcga ttttcggctt gccctgagtt tcgcgcaccg gaggcagatc
     1321 tgccagaatc gcttcggttg ccatatcaat gccattattg ataatccggc aacgccctgg
     1381 cccgtacaga ctattgaaag cgtcagccac atgctggctg ggggaaataa actgacagcc
     1441 cagcgccagt atctcgcgga ataactggcg tttgcccgcc accagctggt gcgcgcgatc
     1501 aatcttcacc ggcggatagt tatttaaggt cgggcatttc tggcagcccg ttttccagcc
     1561 ttcgcaaccg tcggtaaagg cgcagcgtcc ggttacactc cagtggtcgt gcagcgtcca
     1621 gaccagggtg acatccggtt tatggttttt cactttttcg caaaagcgca ccacgctgtt
     1681 aagattcaac cagtagctgt gcagcacatg aaaatgcagg accaccggac ccggtgtgcg
     1741 agtaatggtg cgatataact cattgaaatt gccgaacaga tcgcgattaa acagacgaaa
     1801 caacgcaatg ttcgccatcg cggtcatccg cggcgtatgt ttgatgacct gcggatagtt
     1861 ctgatggctg acgctctctt tgccgccttt gccgtagccg tagacaaaat gtgacgccag
     1921 cccctgttgc agcgcacgct ggtggagatc taacgccaca cctgctgccc cgccttccgc
     1981 cagtcgcaca ttaaattgca gaatattcat ttaatcacct tcactcgcgc tttttctccc
     2041 accaccagcg cgttgtccgg gacagagtcg agcaccacgc tgcccgcacc cacggtgacg
     2101 ttgttaccaa gcgtgatatc accaagaata atgacgttgg caccgagttc gacgccgttg
     2161 ccaatgtgtg gacatgccat gttatcggca ccccgattgc cgatagtgac gccgtggcga
     2221 atggtgaaat catcccccgc caccacgttt ttattgatca ctacggcgta accgtgatgg
     2281 atagtaaagc ggcggccaat ggtcgcggcg gcctggattt cataaccgaa aaagcattcg
     2341 gtgataatgc gatacagcac cagcagcggg gccgcccaca gattgttgag gacgttcttt
     2401 ttgcgccaca ccgaacaaaa atgagcgacg cgataggcaa gtaccatgca gcacgggcgt
     2461 aaactccagc tgttggcgcg cagatcttcc agcatcttta gcgcccccga ataccatcag
     2521 ccagacgttt gccgttacgc accgacagca gcgtcaacaa ggtgcgccag gtcatacgtt
     2581 tattgcggat ctggtagagg gtaaacagct gatatttttt actggcgcgg tcgaatttgt
     2641 ctttgtgctt gcggtaaaag tggaagtatc cggagaattt tttcggtgac gaggtgatct
     2701 gcatttcgcc gtgattgata tgcaggatct gcgtcgcctc ttccactttc cacggttcgc
     2761 cgtactccac caccatccgc aggaatatgt cgtaatcctg tgctgctttc agttcggtat
     2821 cgaacaaaca ctctttgaaa cgccatgccc aggtaaagac ctgattgcca atgatattac
     2881 gtttatagaa caggcggcgt gagtacggcg atttgggata cagtggtaag ctcgccggtt
     2941 gggaataaac ttctccctgg caaacgtagt cgttagcgta caaaaaggcg tgtgtgacca
     3001 gttgctgttt atgggcgagg aagacgctca ggcggttggg cgtccattca tcatcatcat
     3061 cgatcccggt gatgtattcc ccttgcgcca gcataatcgc ctggttacgc accgcgcacg
     3121 ccccgctgtt aatgtcgttg tggatgtaag tgatacgcgg atcgttgagg gcagtgacgt
     3181 actgttgcag ttgttcccag gaggtggagc aatcatccac gatgatcatc tcccagttgc
     3241 tgtagtcctg gcgcaggacc gattttatcg cccgaatcgc cagttgttgg cggttccagg
     3301 ttggcatata gattgagatc agcggattgt ctttcatagg ttgttctccc ggcttatgag
     3361 ctggtttgct tgccggatgc ggcgtgagcg ccttatccgg cctacggggc ggtgcgaatg
     3421 caggcgtcgt tatttcgcat ccgacttata ttcgtattcg taatagccat aatcctgata
     3481 cgcgctggcg cggcggaaga tggagttcag aatcacccct ttcaccggaa taccgttttg
     3541 ctcaaagcgg ctcagactgg tttccacttc tttcaatgtg ttgaccgcat aacgcgccac
     3601 cattaacgtg gttccgacat gacgaccaac aattgccgca tcggtcactg ccagaatcgg
     3661 cggcgtatca atcaacacca ggtcatagtt tttactcgcc cagttcacca gttcggcaaa
     3721 gcgttcgctc attaacagtt cagaaggatt tggcggcacc tgaccgcgcg ggatcaggtc
     3781 aaatttggca atagaggtcg gtttagcagc ggtagtaata tcgccctgac caatcagaat
     3841 ctccgacagc ccattgacat tattggtgcc caacagctcg tgggtgtagc ctttgcgcat
     3901 atcgcagtcg atcaacaaca cgcgtttatt ggtctggctg atcaccgccg ccaggttggc
     3961 gcagacaaag gttttaccga ttgacgggct aaccccggtc atcatcaaca cattgttctg
     4021 cgcctgcatc atcgcgaagt gcagactggt acgcagacta cggatggctt caatcgccag
     4081 atcggttgga ttccccactg ccagtagctg gctctgttta tagcgtttaa cccctttgat
     4141 ggttttgacg ctatcgcgcg ctttctgcca ctccgacagc gggatgctgg catagacgct
     4201 gattccgtgt tcttccagca cttgcgggct ttcgatgccg cgattaaaca acgagcgcag
     4261 cagcacaccc acgatagaga gcatcaggcc aaggataatc gcgccgagga taatcagccc
     4321 tttcttcggt tttagcacgc caggctgagt gattgccggg tcaataatgc gcacatcgcc
     4381 gacggtgctg gcttcggtga ttttcagctc ctgctcttta ttcagcagtt gcatatagac
     4441 ctgctgacca gactcgacat cgcgggtcag acggacaatt tcctgctggt ttttcggcat
     4501 cgccgttacg cgaccgttaa gtttggcttt ctcttcttcc agcgcctgac gtttctccag
     4561 cagcgtgcgg tacgccgggt gaactttggt gtacagcttg gagatttccg cctctttaaa
     4621 ggtcagttcg ttcaactgcg cgtcgatgtt caccatcgaa tcgagcaccg ctttcgcttc
     4681 aagcggcaga tcaacagaat ctttatcctg acggaaggca ttcagtttgt tttcggcaac
     4741 atcgaggcgg ctacgtactt ccggtaactg ttgcgcgagg aaagcgaggc ttttcgacgc
     4801 ttccgccgat ttgcgctcaa tattttgttc ctgatagtta cgagcgatgc tgttaagaat
     4861 gtcgcggatc tgttcgcgat cttcaccggt ataagtcagg ctcagtacgc ctgcgtcttt
     4921 gccgttctcc gttaccgtca ggctgttttg cagttggttg atcatcccca acgtggagta
     4981 tttggtgacg gtaaactcac tgcccgggcg ggcgtgaatg gcttcaacca tcagcgtgac
     5041 gccttctttt ttcagcatct ggcccgcttg cccacgggcg ctaaagccgc catcactgct
     5101 cagggtgtag tttttgtcat ccagcacatt aagcgtaaac acctgatccg ccatctcttt
     5161 cgggcggtta aaggtagtca ctttcaccgt ctcgttctga cgccccatca ggcgatccca
     5221 gcccgccccg aaaatcggga acgtgttttt gctcaccgca atatcgaggt cgagatcgtc
     5281 caccgtttta ccaagcacca gacgcgaacg aatcaactgg atctcggcgt ccgatgcagg
     5341 cggtttgttg gctaatgccg agccgatatc ctgcaccagc gaattgccgc tgttttgctc
     5401 gatttgtacc agtgcgtcgg cactataaat cggcgtggcg aagaaagtgt aaaccacggc
     5461 acagagggca aatacgacgg tgatgccaat cacccaccag cgcgcttcaa tgacggtgcc
     5521 gaccaggcga ccaatatcga tttcatcact gcccgttacc ggagcggcat gttgttttac
     5581 tttttctgtc attcttatac ctgctctgcg ttcaatgcct gcgcccactg gcgggcagac
     5641 cgttcaagta atgtgtacac cgctgcaaac gtttcccggc ttttgcgata cggatcgggg
     5701 atttcacatt cgttatccca gtgaccaaac agcatcactt tgccgcgcat ctccggtgcc
     5761 atctcgcata agcgttcgat atggcgctta tccatggtca aaatcaggtc atagttgcga
     5821 cacaggcggc ggctgatttg tcgtgcacag tggccttcca gagacagttg atgttctgcg
     5881 gcgacgctga tagcggtagg atcagcgccc ttaccgacca gcgcgccgag tccagcggac
     5941 tccactttca gttccgggtg ataacgttgc agtaagcgtt ccgccatcgg ggaacggcaa
     6001 atattgccga cacagacaac taagatgttg ttaaacatga cgattaccag ttatgaatgt
     6061 cgctggctgt atccgtcatg taacggacac cgctaatagt tggcagcaac tgattgatca
     6121 gacggttcca gcgggaaacc ggggcggtgg tgacatacac cacgtcataa ggttgcaggc
     6181 ggaattctgt cgccatcacc agcgacgtcg catcggacat atccagctgg tagatattgg
     6241 caatcttgcc gttacgcccg ccctcgcctt tcagcggacg aatgacaaag atgccgctgg
     6301 cgttggaggt ggtcatgtcg ataccttcgg cattggccag agcttcagtc agggtcatgc
     6361 cgctaaagtc cattttcagg gtgctctgtt tcttcacttc acccatcaca aatactttca
     6421 gatcatcatt acgcggcacg tagagaatat cgccgggata aagcagtcgg ttctgattga
     6481 ggtcgccgtt ttgcatcagc gcctgtaaag aaatgcgttc ttcacgacca ttgtgtgtta
     6541 gcaccacgtt gcgccagtca gcggtgtcgg tcaggccacc tgcggcgttg atggcgtcga
     6601 gaatggtcag tggtacgttg gtgatcgcct gttgaccgga tttattcacc tgacctgaga
     6661 tataggcctt ttgtgagcgg aaggcggcga tattaacgtc cacctgcggg tcagcgatgt
     6721 acgtcgctaa gcgcccggta atatcactgc ggatttcagc gagcgttttc ccgactacgt
     6781 gaaccttgcc gatatacggg taaaacatag tgccgtcagg ctgtacccag ttgccggtgt
     6841 cgctggagct gcggtactga ccagctggcg tggtgagttc cgggtgatcc cagacggtga
     6901 cattaagaac gtcccccggc ccgacgcgat actggtaatt cgcgatctcg ctttccagcg
     6961 tcatattggg gcgcgctaca ttcgggcgtg ggcgtaattg gtcaatcagg cgcggggtca
     7021 gcggataaac attcaccatt ttgtcgagat cgaaatcagc gtcctgctgt ttgatgacgt
     7081 ctttgcccat cgtcgacata ttgctgcccg gaagtactgt gcaaccgctt atcaaggtta
     7141 ctgacaccaa taatggcatc aatttcattt tggatttcat cattgtttat ttatcacttt
     7201 ggcagagtaa ttatcctgtg cactattaat agcaatgtcg ccatgcacat ttaccttgca
     7261 gttaattgaa taaaaattta actggcatca gtcctaaaaa aattgatttc atccgcaggc
     7321 tattgacaga ataattcaga ctggtctttc aggcatccag acacgctacc gcccctggct
     7381 ttttagctac caatacactg attgagctta atttttcaaa ccctctctgc atacaatcgt
     7441 tgatgagaaa gggttattgc ggaaattaac ttccgaatat aaggtgacat tatggtaatt
     7501 gaatattggc tcgccaataa tgcaggagga agtgttacag ctaacggaat agcaggcaag
     7561 gtaacaattc ggcaattggc tatttttaag aattatatta agtatcattc aatatggttt
     7621 ttaggagttt ctttaggttg acaatattta atattgcgtc tccacatgcg atatttctta
     7681 aataatgttt tattattacc gcttttaatt cagggataat gtgaggttat taccctcaaa
     7741 taatatgaga taaatagtgc tgcaacattg catttttgcc ccgatttatc catgatcgaa
     7801 ttgtgacatt tgtcatacaa cgaataggtt ttgtacttac tatggaatgg attgccgatc
     7861 cgtctatctg ggccgggtta atcacgctga ttgtgatcga actggtcctc ggcattgata
     7921 acctggtctt tattgccatc ctcgccgaaa aactaccgcc gaagcagcgc gaccgcgcac
     7981 gggttaccgg gctgctgctg gcgatgttaa tgcgcctgtt actgctagcg tcaatctcct
     8041 ggctggtcac cctgactcaa ccgctgttca gcttccgctc gtttaccttt agcgcccgcg
     8101 acttaatcat gctgtttggt ggtttcttcc tgttgttcaa agccacgatg gagctgaacg
     8161 aacggctgga agggaaagac agcaataatc ccacacaacg caaaggtgcg aagttctggg
     8221 gcgtggtgac gcaaattgtg gtactggacg ccattttctc actcgactca gtgattaccg
     8281 ccgtcgggat ggtcgaccat ttactggtca tgatggccgc cgtggttatc gctatcagcc
     8341 tgatgttgat ggccagcaag ccgttaacgc aatttgttaa cagtcacccg accatcgtta
     8401 ttctctgctt aagcttcctg ttgatgattg gctttagcct ggtggcagaa ggtttcggct
     8461 tcgtcattcc gaaaggctac ctgtacgctg ccattggttt ctcggtgatg atcgaggcac
     8521 tcaatcagtt ggctatcttt aaccggcgac gttttctttc cgctaatcag acgctacgcc
     8581 agcggactac cgaagcggta atgcgcctgc ttagcgggca aaaagaagat gcggaactgg
     8641 acgccaaaac cgcgtccatg ctggtggatc atggaaacca gcagatcttt aatccgcagg
     8701 aacggcggat gattgagcgg gtacttaatc ttaaccagcg taccgtaagc agcattatga
     8761 cgtcgcgcca cgatattgag catatcgatc tcaacgcgcc ggaagaagaa atccgccagt
     8821 tgctggagcg aaatcagcat acgcggctgg tggttaccga cggtgatgac gcagaagatt
     8881 tgctcggtgt tgttcacgtt atcgacctgc tacaacagtc actgcgcggc gaaccgctca
     8941 acctgcgggt gttgattcgc cagccgctgg tgttcccgga aaccttgccg ttgttaccag
     9001 ccctggagca gttccgtaat gcccgcacgc actttgcttt tgtggtggat gagtttggct
     9061 cggtggaagg gattgtgaca ttaagtgacg tcactgaaac cattgccggt aacttaccga
     9121 acgaagtgga agagatcgac gcccgccatg atattcagaa gaatgcggac ggttcctgga
     9181 cggcgaatgg tcatatgccg ctggaggatc tggtgcaata tgtgccgctg ccgctggatg
     9241 aaaaacgtga atatcacacc attgccgggc tgttgatgga gtatttgcag cgtattccaa
     9301 aacctggcga agaagttcag gtgggggatt atttgcttaa aacgttgcag gtggaaagcc
     9361 atcgcgtgca gaaggtgcag attataccgc tgcgtaagga tggcgagatg gagtacgagg
     9421 tgtgatggta tcggccattt tacccaatgc tgcgtaacgc cttataactt cagcaatgaa
     9481 gcgttgtctg tggtaaacgg acatcacagc gggggtgtgg gtccggctgt aaatcgccga
     9541 atcgtttcct gtaggctggg gcagcccaca tggatgtggg ctgagggcgc gttttacagg
     9601 gatgttacct cgcgcccgta cccgatagcc gaaaggaata agatgaggga accgcgccag
     9661 cggcgattta ccgccgggag cccgggttgc cagggtggtg gcgattgagc caccctggca
     9721 cgttcaccgg cttgagcgtt ccatagtagc gaggaacatc aagtgaacgg aacagccttc
     9781 gatcttacat attcagaata taatagtgcc gccgcgaacg cctgatgcga cgctttacgc
     9841 gtcttatcag gcctacagga caggtcacct ggtaacctac atcttctcca gcaacttctt
     9901 cacatctttg ccattgcggg aatctttatt ccgctcggcc cagtcattca ggcgacgttt
     9961 cgcttcgtcc tgtagatgtt tgcgcagtaa ctgatccact tgcaggctgt aattgagttg
    10021 ctgccagttg ccataaaccc gcagcggtac tggcgtttct ttgaggaaat caatcagttt
    10081 gctttccccg ttccagccac ccacgacccg aatatcaaac tgggtgtcgc aggtttgatc
    10141 tgccagattc aacatgcctt cccctgtcag cgccagcaca ggcgattgac cttgcatgtc
    10201 gtttaacgtc acgacgccat ccttcaacgt caaatcggtg gtaaagcggt caagccgcgt
    10261 cacgttatcg aagttttcag cggccttcac atcaccgcca ttacgctcta ccgcttgctg
    10321 aatcatctgc tggaagttca tcccttccat acgtgtgtcg gtcatctcaa catgcgcctg
    10381 tccttgccag ttgtggcgga atgcgtcggc atctatgtca gcaccggaga aatcaccagc
    10441 cagtgacatt tttccggtca acgaaatcgg atagttaaac gccttcagaa tggtaccaat
    10501 ctcaacgttc tccagccgtg gctggaagtt tatccgcgga tttattgatg tcgcgtccag
    10561 cgtgcccggc agtgaaacct ttccaccgtt aagcttgccc tgcagttgag taatttccag
    10621 caaacccgac ttgttggtca tttgcgtggc aacatctgta aaattcattc cgcgccagcg
    10681 cacgttactg gcctgcaaca aaatatcagc tgtaaagcct tgcagtccct gataggccgg
    10741 ttcatcaata cgcgaagaaa tgaccgggcg cggcaacgtt gattggctct gcccctgctg
    10801 cgcggcaccg ttttcaccat tcgcggtttc attaagcggg atgaggttgt caagattcag
    10861 ttgcgggaat tgcagtctca gctgccattc cggtttctct gtcagcgtga cctgtgcttg
    10921 tccgctcagc gtactgtcat tggcggtcaa actaatttgg ttaaatgaaa ggcgtttatg
    10981 tgactcctgc cactgggact ggaaactccc ctgcccctga ataccttgtt ttggtaaatc
    11041 ggcaccctgc aactgccagt taatttgttc aatagccgct gttaaatcat gcggataatc
    11101 agaagcatct accgtaccgt taagggatat cgtgagatcg cgctgatcgc gattaacccg
    11161 cccggagaac tcaaatgagc cacgatgttg gggatcttgt tccatttgca ggcggatatt
    11221 gcggattgtc acctgctcgt catcttcatg ctggaacacc agaacgctgt ccgccacctt
    11281 aagactggat atatcaaacg accatccgcg atcgtctgac agatccggca aggtattgtc
    11341 gcgcggtgca accggagcgt cttcactgcg caccgcttcc gtctgcggcg tcagttggat
    11401 cactgcccct tttagcatca cctgcttaac gctcagttga tgactcagta gtggtaaaag
    11461 cgccacgtcc agacgcatgt tgtcggcgcg aaccagtggc tggcttgcgc cctgggcggt
    11521 gagagacatt cgcccggaga ggatactaag ctgcggccag acgtgccaac gcagtggccc
    11581 gtcgagctgc aattgataac cgctacgtgc agcaacttgc ttgaccatat agtcgcggaa
    11641 atcattcgga ttcaccagca acactaacgc agataacccg gccaccagca cgaccaagag
    11701 tatcatcagc gtcgtcagaa atcgtctcat ggtatcctca atgggcctga attagtcttt
    11761 atcgattcgg ctggctaccg cgccctgctg gttgcgatat ttcgcatctt cacggcggtt
    11821 gtaaggtcgc gccgccgggc cggaaagcgg ctcaaagctc agcgcaccaa ttaacatgcc
    11881 cggacgcagc gccagcggca gcttaccgga gttgtagaac tccagcacaa tgcaaccaga
    11941 ccagcccgga tcgatacggt gcgcggtgac gtgcaccatc agccccagac gcgccaaaga
    12001 ggaacgcccg tccagccagc ccaccagatc ggctggcagc gtcaccgact ccagtgtcac
    12061 cgccagcgcc agctctcctg ggtgaagata gaacgcctcg ccctcgtcga gaacgatctc
    12121 atcgctcatc acgcggtcaa gcgcggcgct cacttcatct ttgggaccgc tcagatcgat
    12181 aaacgctgcc gtgtgaccac ggaaggtacg aaatttattg cccaggcgta catccaccgt
    12241 cgcgccgtta atacgctcca ctggcggacg tgggttgatc gacaaacggc cttcatcaag
    12301 ccaggcttca atatctcggt cacacagacg catggcattt tctcctttcg cacatcactc
    12361 ccttaacgcc aattacgtca agggcatacc aacttatcac tgaacggtac acaatttatc
    12421 aagcttattc aaagaactga cttattttcg ctttcaatat atcgatcgcg atgcggtttt
    12481 tcccgccgcg cggcacgata atgtccgcat attgtttaga aggctcaatg aattgcagga
    12541 acatcgggcg cacggttttt tgatattgcg ccatcacgga atccattgaa cgcccacgct
    12601 cgttaacgtc acgcttgatg cggcgcatca ggcagatatc cagcggggta tcaacgaaaa
    12661 tggagaagtt aagttcgtca cgcaaacgcg catccgtcag caacaaaatg ccttcgagaa
    12721 tgatgacctt cttcggctca accgtcaccg tttctttcat acgcgtatgt tcaacatagc
    12781 tgtaaaccgg caggtcaatt gccgagccgc gtttcaacgc ttgtaaatgc tcaagcaaca
    12841 ggctgtgatc catcgcgctg ggatggtcgt agttggtctt aacgcgttct tccatcgaca
    12901 gatggctttg atctttgtaa tagcagtctt cgggaattac gccgatgtgt tcatcaccga
    12961 cttgctcacg taattcacga taaagggtac tggcaataag actcttgccg gaagccgatg
    13021 cgccagcgat accgataatg acgcactgat gagactgatc agtcatatat ttagcgacct
    13081 gattaacctg gatgttagga agggggcgac gaagcgccaa acgcggcaat tatagggatt
    13141 tcatccgtct gataccagtc gaatagcgtt gccgcgcgct cagagttaat tgttgacaaa
    13201 gaattcccgg tggcaaatta cgttgatcag ttttatgtaa ggtaaaaaat gttatacgca
    13261 gttgcgcaaa ttatccgcct ttacgtcact ttatgagcaa ttcgcatata aaatgtaaaa
    13321 cttttgtact agcataaaca cagaaacgaa tactggcgac ctggccttgc ggataaagcg
    13381 gtaataagca aacaatcaca gcatgtatta attgccctgc cccacccgct gcttcacctg
    13441 gtcagtttag gtttagtctc gtttatcttt acccttgtct cgcttgagct ttcgcagttt
    13501 ggcacccaac tcgccccact gtggttcccg acgtccatca tgatggtggc gttttatcgc
    13561 catgccgggc gcatgtggcc gggaattgcg ctgagctgtt cgctgggaaa tatcgccgca
    13621 tccatcctgc ttttttccac cagctcgctg aacatgacct ggacgaccat caatattgtt
    13681 gaagccgtgg tcggggcagt gctgctacgt aaattgctgc cgtggtataa ccccttgcaa
    13741 aatctggctg actggctgcg tctggcattc ggcagcgcca ttgttccgcc tctgttgggg
//
LOCUS       DAEEUE010000102        13669 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.102, whole genome shotgun sequence.
ACCESSION   DAEEUE010000102 DAEEUE010000000
VERSION     DAEEUE010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13669
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.102"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(337..582)
                     /locus_tag="KLK84_003251"
     CDS             complement(337..582)
                     /locus_tag="KLK84_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010474.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1227..1302)
                     /locus_tag="KLK84_003252"
     tRNA            complement(1227..1302)
                     /locus_tag="KLK84_003252"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1267..1269),aa:Phe,seq:gaa)
     gene            complement(1408..2115)
                     /locus_tag="KLK84_003253"
     CDS             complement(1408..2115)
                     /locus_tag="KLK84_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBE2010475.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2513..4648
                     /gene="speC"
                     /locus_tag="KLK84_003254"
     CDS             2513..4648
                     /gene="speC"
                     /locus_tag="KLK84_003254"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HBE2010476.1"
                     /translation="MKSMNIAASSEVVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4697..5953)
                     /gene="nupG"
                     /locus_tag="KLK84_003255"
     CDS             complement(4697..5953)
                     /gene="nupG"
                     /locus_tag="KLK84_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HBE2010477.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="KLK84_003256"
     CDS             complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="KLK84_003256"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HBE2010478.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7299..7574)
                     /locus_tag="KLK84_003257"
     CDS             complement(7299..7574)
                     /locus_tag="KLK84_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HBE2010479.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="KLK84_003258"
     CDS             complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="KLK84_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBE2010480.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            8815..9534
                     /gene="trmB"
                     /locus_tag="KLK84_003259"
     CDS             8815..9534
                     /gene="trmB"
                     /locus_tag="KLK84_003259"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBE2010481.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9534..9860
                     /locus_tag="KLK84_003260"
     CDS             9534..9860
                     /locus_tag="KLK84_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HBE2010482.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            10044..10763
                     /locus_tag="KLK84_003261"
     CDS             10044..10763
                     /locus_tag="KLK84_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HBE2010483.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            10939..11985
                     /gene="ansB"
                     /locus_tag="KLK84_003262"
     CDS             10939..11985
                     /gene="ansB"
                     /locus_tag="KLK84_003262"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HBE2010484.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12102..13063
                     /locus_tag="KLK84_003263"
                     /pseudo
     CDS             12102..13063
                     /locus_tag="KLK84_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
     gene            complement(13164..13589)
                     /locus_tag="KLK84_003264"
                     /pseudo
     CDS             complement(13164..13589)
                     /locus_tag="KLK84_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
BASE COUNT         3515 a         3263 c         3539 g         3352 t
ORIGIN      
        1 gctgaggagt ccccagaaaa gaagacaggc atagagtttt gtgatctact gattgcgcta
       61 acaaattcaa tgagatcacc tctatgcctg cccgttaagt atgtcaggct tttttccgta
      121 atgctctaac ttccacgcat tggtttctga catcaaataa ggctatgact ttctctccct
      181 tttaatctca ttctgatcta aaaccatatc ttaaaaaaat gcatgggtat tttaattatt
      241 ttgatgttga tctaacgcac tcaaggacca ttttattacc gattatccat tctatttaac
      301 tgttgcgata taaatgtttg ttttaggtgc tttaattcag tacaagtcat caaaccgtac
      361 cagaccctga ctgtcagtga caccgacagg tgggggcggc ggtgtggggt caaactgagt
      421 gtgtctgcct ctggcccaca gagccagctc tgccgccatt gcccgtggat gatactccag
      481 ctgcacgttg agaccggaaa ctgggttgtt gttctcatcc acgaactgca gcgccagcca
      541 gtgcatccgc tgcttaccgc aggcgacttc tttgtacttc attttgtgac tcaggcaggt
      601 gactgtcgtg ttgcctgttg cgtccatttt gcggttaaac ccttttaatt caatttgtta
      661 aattgctttc caccttttgc ggggcgcgac ttaaccaaaa tattcactgt tatcataata
      721 tgcaatgggc tggaggttgc aggtcattta gggattttct gtaagccccg ttttgcagaa
      781 cgttctatct ccgattgaga tcctgtctct cggtattacc ggtagtctgg ccataatccc
      841 ctatggatca tattgatata gtaggcataa cacccaaaag ttgactaaag ctaaatcaat
      901 cagagtgaaa aatgttcggt ttgatgtagg gggggactga ggaggggcca acgcaggtgg
      961 tatgcgtttt acttcagacc ggacaaagac cggatgtatc cgtgcagtaa tgaatccgtt
     1021 agccaggtgc tcatgtggaa atagtgtcta ttggggattt gaagtttgac agaaaaggcc
     1081 acagtgacca gaagggggct atttagaaga aatgttcagg ctggatggaa gaggtaaagc
     1141 agtgacatca gcattcaaag tgaccggata tctaccagaa ccccagaaag caaaaaacca
     1201 gcccgtaggc tggttcttct aaatagtggt gcccggactc ggaatcgaac caaggacacg
     1261 gggattttca atcccctgct ctaccgactg agctatccgg gcaacggggc gcattaaacc
     1321 tgattcgcct cgtctcgtca atcaaatttc ttcaatttac tgcagactgc acaatctatc
     1381 atcactttgc cgctattgca cgcattgtca ggcaaaccag gcggtccagg cggcggaaag
     1441 cggcattgcc agaaggagtg ctggaagcat gttgaccacc gggaacattt taatgccaca
     1501 gatgcgtaat ccggttgcca gcagcaataa accgcctacg gcgctgaagt ctgccatcat
     1561 cgacggtgtg atcagcggta atatcagcgc ggcagcccac gccagcgtta actggatgat
     1621 cagtaatggg atactaatta ccgacaccgc aatacccagc gagcaggcga agatcatcgc
     1681 cgtaaagaaa tcaagaaatg acttggcgat taaaatactc ggatcgccgg tcatcccttc
     1741 gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac aggacaataa tcgcgacata
     1801 attctgaata aaagattcat gcgctggctt cttacgtgag tgacgaaaca gattttgtgc
     1861 tttggcgact gctgtattga cacctttctc cagcaaacaa atttcgccaa ttagagcgcc
     1921 aagtagcgta gctaaaacca tcgctggaag gttggcgcat ttcacaacca gtaaaatacc
     1981 aatccccagc gatgccagac caaaaataga tgtcatggag acgcggatac gttccggtaa
     2041 gcgttggctg agcagtgcgc cgagaacgcc acccagtaag actgcactag cgttgataaa
     2101 agggccgatg accacaagag ttcctgttag ctgtattgtt tgattttctt attatgaact
     2161 ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg cttgcacgcc ttagcgaaag
     2221 gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt ttgcccgatg cacgccatct
     2281 ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt ttccctttct atgttactgc
     2341 tcatgcggtg aagttaacgc acgctcactg caggacaaca gtaaaatcag agcgtttctg
     2401 cttttactga tgtctggcgg tcggagctgg tgaccagttt gacccatatc tcatggggca
     2461 gggttttcca ccttgtccgg tattcttact tccccgaaac gggtttgcgc ttatgaaatc
     2521 aatgaatatt gccgccagta gtgaagtggt atcccgactt tcttctcatc gccgcgtggt
     2581 ggcgttggga gatactgatt ttacggacgt cgcggcagtc gtcattaccg ctgcggatag
     2641 tcgcagtggc attcttgcgt tgcttaagcg caccggtttt catctaccgg tgtttttgta
     2701 ttccgaacat gctgttgaat tacctgcggg cgttacggcg gtaatcaacg gcaacgagca
     2761 gcagtggctg gagctggaat ccgcagcctg tcagtatgaa gagaatttgc tgccaccgtt
     2821 ttatgacacg ctgacgcagt acgttgagat gggcaacagc acctttgctt gccctggaca
     2881 tcaacatggt gcgttcttta aaaagcatcc tgccggacgc catttttacg atttctttgg
     2941 tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta aaattgggcg atctgcttat
     3001 tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc aaagtctttc atgccgataa
     3061 aacctatttt gtgctgaacg gcacatcggc agcgaataaa gtggtgacga atgcgctgtt
     3121 aacgcgtggc gatctggtgc tcttcgaccg taacaaccat aagtcgaatc atcacggcgc
     3181 gctgattcag gcggggacga cgccggtcta tctggaagct tcacgtaacc cgtttggttt
     3241 cattggcggt attgatgcgc actgttttaa tgaagagtat ctgcgccagc aaattcgcga
     3301 cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc ctgacgatta ttcagctggg
     3361 aacctatgac ggcactgtct ataacgcccg tcaggtgatc gataccattg ggcatctgtg
     3421 tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa cagtttatcc cgatgatggc
     3481 ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat ccggggattt ttgtgaccca
     3541 gttggtgcac aaacagcagg ctggattctc acagacgtcg cagatccata aaaaagataa
     3601 ccatatccgc ggacaggcgc gtttttgccc gcataagcgg ttgaataacg cctttatgct
     3661 ccatgcttct accagccctt tctatccgct gtttgctgca ctggatgtta acgccaaaat
     3721 tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc gttgagttgg gtattgagtc
     3781 gcgcaaggct attcttgcgc gctgtaagct gttccgcccg tttatcccgc ccgttgttga
     3841 tggcaaattg tggcaggatt atccgacatc agtgttagcc agcgaccgcc gttttttcag
     3901 ttttgagccg ggggcgaagt ggcacggctt tgaaggatat gccgcggatc agtattttgt
     3961 tgatccgtgc aagctgttac tcactacacc aggtatcaat gccgaaaccg gcgaatatag
     4021 cgactttggc gttccggcga cgattctggc gcactatctg cgtgagaacg gcattgtgcc
     4081 ggagaagtgc gatctcaact ccattctgtt cttattaact ccggcggaaa gccacgagaa
     4141 gctggcacaa ctggtggcga tgctggcgca atttgaacag catattgagg atgactcgtc
     4201 gctggctgag gtgttgccga gcgtttataa caaatatccg gtgcgctatc gcgactacac
     4261 cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc agtttcgacg tcaaagacct
     4321 acaaaaagcg atgttccgcc agcagagttt cccgtcagtg gtgatgaacc cccaggatgc
     4381 gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg attcgtgatg ccgaagggcg
     4441 aattgcggca gaaggggcgt tgccttatcc acctggcgtg ctttgcgtgg tacccgggga
     4501 agtctggggt ggggcggttc aacgttattt ccttgcgctg gaagaaggcg tgaatttgtt
     4561 gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa accgatgcgg atggcatgaa
     4621 acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac gggtcacctt ctggcgaccc
     4681 gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc tgtcgggaca cgaacgtgtt
     4741 tatatttgaa catcgccatg aacgcgaagg ccagaaccac ggaataacca gcgaaaatca
     4801 accatacggt ctgccagtcg gtaatgccgt tttgggtgta catctcaaca actttaccgc
     4861 tcacgatgcc gccgaggata cagccgaagc cgttagtcat catcaggaac atcccttgtg
     4921 cactggcgcg aattgccggg ctaacttctt tttcgacaaa caccgaacca gagatgttga
     4981 agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac cagcagtaca gtaccgaacg
     5041 gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat ccacgccaca atactgatca
     5101 tcattacgtt cttaataccg tagcggctta agaagaacgg gatggtcaga atgaacaggg
     5161 tttcagagat ctgcgaaatc gacatgatga ttgacgcatg ctgcacgatg aagctgctgg
     5221 caaacatcgg atctttgtcg aagctgtgca ggaaggtatt accgaacatg ttggtaatct
     5281 gcagttccgc gcccagcagc attgagaaga tgaagaagat tgccatacgc ttgtttttaa
     5341 acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct ctgattcgct tgctgtttag
     5401 caaccggaat atgcggcaga gtcagggtaa acagaaccag aatggcggaa agtgctgcgc
     5461 caatatacag ctgcatgtgg cttaattcga agccagacag gctcaccacc cacattgcca
     5521 tgataaagcc gatggtgccc cagatacgga ttggcgggaa gtcagtaacg atatccatcc
     5581 cggcattttg caggcgatag taagagatgg tgttgattaa cccaagcgtt ggcatataag
     5641 caaacgagtt aatcaatatc acaaggaaca tcgcttccgg cgtcgtgacc tgtgccgcca
     5701 tgaacagcgt gatagcgcca atggtgtggc aaatggcata tacccatttc gcacttaacc
     5761 atttgtcggc cacaatcccc agcagcgcag gcataaatac cgcggcgata cccagtgagc
     5821 tataaactgc gccaatagaa gcaccgtcaa acttcagggt aacaaacata taggagccga
     5881 gggtcgtcag ccaacttccc cacagacaga actgcagaaa agagaggatt ttcagctgca
     5941 gcttaagatt catgttaatt tcctcacatc gtgatgcgga tgagtgtttg aaaaagcaca
     6001 tttgaccgtg cgagatgatg ggattctatc aacttcgcgg actggttttt tgttacctgt
     6061 ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat tgtgatgtgg ataacatttt
     6121 tgcccctgag catcgtcagg ggcggttaat ggaattatcg gcggcggtag gatttttgcg
     6181 cggtattcac tttataaaga taacggcgag attccgcaga gggatggcgg gtcgtcagcg
     6241 tctgataaac atcgcccggc gtcatggtgt taataatatt ggcagcctga atcttatcat
     6301 tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata ggcggtgatg acggcataac
     6361 gccgcgatgt tgggttatca attccgccga gataaacatt gttcagcatc gccagatacg
     6421 cggtgccggt atcaatattg ctggcaggat caaacaagaa actgcggctc ggcgtgccgg
     6481 atttcccctg cgagcggaac acatctttcc cggcagtatt ttgtaccacc tgcattaatc
     6541 ccagcgcatc ggaacggctg accgcatacg ggttaaagga agattcagtc tgcataattg
     6601 ccagaatcag cgactcatca acgccatatt tccgtgacgc ctggcggacc atgccgagat
     6661 atttgtgcgc acgtttatca aggtggttcg gcaccatgtt aatggtgacg ctgtagatga
     6721 tacgtagccc gttgctgcgg cttttcagac ggtttttcag cagataatcc gcgaagttgc
     6781 ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc caccacctga ccgtaaagga
     6841 aaggttcttt cgaaattgta atatcatcaa cgtcggaata gagatcgacc gaactcggat
     6901 cgtcacccat cagcaacgtt ttgataattg cccggcgcaa atgcgcggca ggttctgtcc
     6961 cggcgatggt ttcgatagta atcgtaccgt catcgaagtt gatgtggctg cgggtctgat
     7021 attgatcggt gtatttcacg tagtccttag gaccggcgat caccacctct ttgaagcccc
     7081 agatgttctc aatattgtgg gcaaattgcc ccatcagaat atcaaaaccg ttggtatctt
     7141 tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt cgaacaggag atgagcaacg
     7201 gcgcaatcaa agccagcgcg agatattttt tcatcattcc gggtgcgtgt tgtgtttgtt
     7261 tttatgcaag ctgccggagg cgtgctccgg cactgttttt attttttatc ttccggcgta
     7321 tagccctcga tatgcacctc tttaccctcg aacaggaagt tgaccatctc ctgctccagc
     7381 agcttgcggt gctcggcatt catcatgttg agtttctttt cattaatcag catggtttgt
     7441 ttgtgctgcc actgcgccca ggcttctttg gagatctcgt tatagatgcg ttttcccagc
     7501 tcgccggggt acagctgaaa atcctgacct tctgcttcac gttgcaggaa agtacaaaaa
     7561 atcgttctgc tcataaatca tcctctttat cgactcacgc gctaaaccgg cgcgccagtg
     7621 cgtaactgct gtaataaacg ctccacggga gccgccaggc caactgacgg cggttgcgct
     7681 aagttatacc agagcgcatt gccttcatcc atgcagccgg tgaatgacga cacgggaagc
     7741 cacataggca caatatctaa gtggaaatgg ctgaaggtat ggcgaaacgc ggtcagttgc
     7801 gtcaggttat cggcagcaat ctgccgttgt gccagccaat gccgcaaact ttcttcgtcg
     7861 gcaaactgcg ggaaacagta taaaccgccc cacaatccgc tcggcggacg ctgcgccagc
     7921 aatacttcat cttcgtgctg taacaggaga aagtagccgg tgcgctccgg cagcgtctgt
     7981 ttcggttttt tgcccggata aagcgaccag ctattgttgg tggcggcaat acatccgttt
     8041 tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc aaatcatcgc gcccaaatcc
     8101 atcatcgcct gattaaaccg ttccacgccg accgcgggcg tcacctgctc gctcaaactc
     8161 catagtttat tctcgacctc ttttttccca ggccagccgc ttacagcata gcagcgcgcc
     8221 agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct tacccagaga aagcgagaga
     8281 atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg cgacttcttc aaaggtttcc
     8341 gggaatttac caccgtgtaa ggtcgccact tgttgtgccg ctttatgcag gttgcgcgcg
     8401 cgggcgtaat agccaagccc ggtccacaag tggagaactt cgtcgagcgg cgcattggcg
     8461 agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa aataggggat aacggtcgca
     8521 acctgagttt gttgcaacat cacttctgag agccatactt tgtagggcgt cttgtcaatt
     8581 tgccagggca gcgttttccg cccgtattta tcgtaccagt ccagaacctg ggctgaaaat
     8641 tgcgacgctt gcatggtcac cgacttcact gttgttgggg gcaagattgc agcacagcga
     8701 cggcagggtg taaaccggaa ctttccgcag ccacggcctt atcatgcttg catccggcaa
     8761 ctaactttgg ataatgcccg ttttcagaac actttcacaa gcgactaaat ctttatgaaa
     8821 aacgacgtca tttcaccgga atttgatgaa aacggccgcc cactgcgccg tatccgtagt
     8881 tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac atgcgctgga aaactactgg
     8941 ccggtgatgg gcgttgagtt cagtgaagat atgctggatt tccccgcgct ttttggccgt
     9001 gaagcgccgg tgacgcttga gattggtttt ggcatggggg cgtcgctggt ggcaatggct
     9061 aaagatcgcc ccgagcagga cttcctcggc attgaagtgc attcaccggg cgttggtgcg
     9121 tgcctggctt ctgcgcatga agaaggttta agcaacctgc gcgtgatgtg tcacgatgcg
     9181 gttgaagtgc tgcataaaat gattcctgac aattcattgc gcatggtgca gctctttttc
     9241 cctgacccgt ggcacaaagc gcgccataat aaacgccgta tcgttcaggt gccgtttgcc
     9301 gaactggtaa aaagcaaact gcagctgggg ggcgtattcc atatggcgac cgactgggaa
     9361 ccttatgcgg aacatatgct tgaagtgatg tcttctattg acggttataa aaacctgtca
     9421 gagagcaatg attacgtacc gcgtccggca tcacgtccgg tgacgaaatt tgaacaacgt
     9481 ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg agagggtgaa ataatggcaa
     9541 agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga cgaattccag gaattaggat
     9601 tttcggtggc atggcgattc ccggaaggta catcggaaga acagattgat aaaatcgttg
     9661 atgactttat taacgaggtt atcgaaccga acaaactggc ctttgacggc agcggttatc
     9721 tggcctggga aggtctgatc tgcatgcagg aaatcggcaa atgcaccgaa gaacatcagg
     9781 cgattgtgcg taagtggctg gaagagtgca aactggatga ggtacgcacc agcgaacttt
     9841 tcgacgtttg gtgggactaa gaaagcatac gggcgatgac aaatgcaaaa ctgcctgatg
     9901 cgctacgctt atcaggccta cagagatgca cgatcgagta ggcgggataa ggcgtttacg
     9961 ccgcatccgg catggaaaac gcgtactttg ttatcaatct ggggccagca tatgctggcc
    10021 tgatttgttc ttgagggaag actatgatgc gcaaaatgct gctggcggca gcactttcag
    10081 tgacggcaat gaccgctcac gccgactacc agtgcagcgt cacgccgcgt gacgatgtga
    10141 ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg caatctggtg atcacgccag
    10201 acggcaacgt gatgtataac ggtaagcaat attccctgaa tgccgcccag cgcgagcagg
    10261 cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg gattgatgaa ggcgcgaaaa
    10321 gccgcgtcga gaaagcccgt attgcgctgg ataaaattat cgttcaggag atgggcgaca
    10381 gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca gcttaaagag cagatgaatc
    10441 gcattatcga aacgcgcagc gatggcctga cgttccacta taaagccatt gatcaggttc
    10501 gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg aattttacag gacagcatta
    10561 atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa cccattacag aatgtgctgg
    10621 gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg gaaaaagcag gaaaaagatt
    10681 tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac tctggaagat agccgcaaag
    10741 ccctggtcgg gaatttaaaa taatcctcta ttttaagacg gcataatact tttttatgcc
    10801 gtttaattct tcgttttgtt acctgcctct aactttgtag atctccaaaa tatattcacg
    10861 ttgtaaattg tttaacgtca aatttcccat gcaaagctaa gggataatgc gtagcgtcca
    10921 cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg cacttgccgc actggttatg
    10981 ggttttagtg gtgcagcatt ggcattaccc aatatcacca ttttagcaac cggcgggacc
    11041 attgccggtg gtggtgactc cgcaaccaaa tctaactaca cagcgggtaa agttggcgta
    11101 gaaaatctgg ttaatgcggt gccgcaacta aaagacattg cgaacgttaa aggcgagcag
    11161 gtagtgaata tcggctccca ggacatgaac gataatgtct ggctgacact ggcgaaaaaa
    11221 attaacgccg actgcgataa aaccgacggc ttcgtcatta cccacggtac cgacacgatg
    11281 gaagaaactg cttacttcct cgacctgacg gtgaaatgcg acaaaccggt ggtgatggtc
    11341 ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc cattcaacct gtataacgcg
    11401 gtagtgaccg cagctgataa agcctccgct aatcgtggcg tgctggtggt gatgaacgac
    11461 accgtactgg acggtcgcga tgtcaccaaa accaacacca ccgacgtagc gaccttcaag
    11521 tctgttaact acggtcctct gggatacatt cacaacggta agattgatta ccaacgtacc
    11581 ccggcacgta agcacaccag cgatacgcca ttcgatgtct ctaagctgaa tgagctgccg
    11641 aaagtcggca tcgtttataa ctacgctaac gcatccgatc ttccggctaa agcactggta
    11701 gatgcgggct atgatggcat cgttagcgct ggtgtgggta atggtaacct gtataaatcc
    11761 gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg cagtagtgcg ttcttcccgc
    11821 gtaccgacgg gtgctaccac tcaggatgct gaagtggatg atgcgaaata cggcttcgtc
    11881 gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc tgcagctggc tctgacgcaa
    11941 accaaagatc cgcagcagat ccagcagatt ttcaatcagt actaatcacc tcgtcccggc
    12001 atcgtgccgg ggctttttca cttcagactt acgtccattg ccaattttca ttaccctaat
    12061 gataatcacc ggaataaatt attccgcgcg agggttttcg ggtgaaaaag caatggattg
    12121 ttggcacggc gctgcttatg ttgatgactg gtaatgcctg ggcagatggc gaaccgccaa
    12181 ctgaaaatat cttaaaagat caattcaaaa agcagtatca cggcattctc aagcttgatg
    12241 ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc cacttggtca gcggaaggcg
    12301 atgtctcttc cagtgacgat ctctatacct gggtcggtca gttggcagat tacgaactgc
    12361 tcgaacagac ctggacgaaa gataaaccgg taaaattctc ggcgatgtta accagtaaag
    12421 gaacgcccgc gtctggctgg tcggtgaact tttactcttt tcaggcggca gccagcgatc
    12481 gtgggcgggt ggtcgacgat atcaaaacga ataataaata tctgatcgtg aatagcgaag
    12541 atttcaatta tcgctttagt cagcttgaat ctgcgttgaa tacccagaag aactcgattc
    12601 ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat ggtggcggcg cagaaagcag
    12661 cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat gacccgcgaa gaagccttta
    12721 aaaaaattca ccaacagcgt gatgagttta ataaacagaa cgatagcgag gcgttcgcgg
    12781 ttaaatatga caaagaggtt tatcaaccgg cgattgcggc atgtcataaa cagagtgaag
    12841 agtgttatga agtgccgatt cagcagaaac gagatttcga tatcaacgaa caacggcgac
    12901 agacttttct gcaatcacaa aaactcagcc gtaaattgca ggatgactgg gtaacgctcg
    12961 aaaagggcaa tatccgttaa cgatgaaagt ttcagaaata aacagtaaaa aagtcgcgat
    13021 tttgatgaaa attgacgata tcaaccaagc taatgaacgc tagaaaaaag ataccgaaca
    13081 actgcggcgc aatggggtga ttaagtaact catgttaagt gcctttgccg gataatataa
    13141 aaagttatcc ggcaaattat tttttacatt agccctagta gctgtggaat gaacaaggat
    13201 atttgcggaa tgtaagtgat catcatcaac gctactagca atgcagcata aaatagcagc
    13261 aatggtttga ttaaatgttg aatcttcaca cccgagatag aacaaccaac aaatagcgca
    13321 ctacccaccg gcggtgttaa cagcccaata caaaggttgg ccaccatcat aataccgaaa
    13381 tgtactgggt ccattcccag ctcttgtgca atcggcaaga agatcggtgt aaagattagt
    13441 acagccggtg tcatgtccat aaagatgccg acaattaata agacgatatt aatcagcagc
    13501 aatatgatca gtgggttatc agaaatcccc atcaatgcgt cgctgatcat ataaggaata
    13561 tcagcattag tcattgccca ggacatgccg gtaatgctgc caacttactg atttagtgta
    13621 tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtc
//
LOCUS       DAEEUE010000103        13015 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.103, whole genome shotgun sequence.
ACCESSION   DAEEUE010000103 DAEEUE010000000
VERSION     DAEEUE010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13015
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.103"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..319)
                     /locus_tag="KLK84_003265"
     CDS             complement(<1..319)
                     /locus_tag="KLK84_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HBE2010485.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFD"
     gene            complement(525..1244)
                     /locus_tag="KLK84_003266"
                     /pseudo
     CDS             complement(525..1244)
                     /locus_tag="KLK84_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(1260..1592)
                     /gene="cusF"
                     /locus_tag="KLK84_003267"
     CDS             complement(1260..1592)
                     /gene="cusF"
                     /locus_tag="KLK84_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HBE2010486.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1616..2998)
                     /gene="cusC"
                     /locus_tag="KLK84_003268"
                     /pseudo
     CDS             complement(1616..2998)
                     /gene="cusC"
                     /locus_tag="KLK84_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
     gene            3155..3838
                     /gene="cusR"
                     /locus_tag="KLK84_003269"
     CDS             3155..3838
                     /gene="cusR"
                     /locus_tag="KLK84_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HBE2010487.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTLSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3828..5270
                     /gene="cusS"
                     /locus_tag="KLK84_003270"
     CDS             3828..5270
                     /gene="cusS"
                     /locus_tag="KLK84_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HBE2010488.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5420..7657
                     /gene="nrfB"
                     /locus_tag="KLK84_003271"
                     /pseudo
     CDS             5420..7657
                     /gene="nrfB"
                     /locus_tag="KLK84_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
     gene            7644..10612
                     /gene="nfrA"
                     /locus_tag="KLK84_003272"
                     /pseudo
     CDS             7644..10612
                     /gene="nfrA"
                     /locus_tag="KLK84_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10613..11503
                     /locus_tag="KLK84_003273"
     CDS             10613..11503
                     /locus_tag="KLK84_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HBE2010489.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11686..12447
                     /gene="envY"
                     /locus_tag="KLK84_003274"
     CDS             11686..12447
                     /gene="envY"
                     /locus_tag="KLK84_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HBE2010490.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQREKVMW"
     gene            complement(12493..12569)
                     /locus_tag="KLK84_003275"
     tRNA            complement(12493..12569)
                     /locus_tag="KLK84_003275"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12533..12535),aa:Arg,seq:tct)
     gene            12812..>13015
                     /locus_tag="KLK84_003276"
     CDS             12812..>13015
                     /locus_tag="KLK84_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBE2010491.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3183 a         3373 c         3307 g         3152 t
ORIGIN      
        1 tgtcgaacca gtagtcgaga cactgggcga ccgtcatgga gttctccacc ttctcttcaa
       61 aataagtgcg tggatccgtt cctgagaaat agagctttcg caagtcagca gtgatctgtc
      121 tggcatcctt caaagacagg gatgggtatc gaccaagccc aagtcgatta ggcttgccat
      181 gccagcgata gcggtactgg aactggatga cccccttcgg tgaaattcgt acgctgaggc
      241 catcagcatc agccacttct tgtgggccag aatatggttt accataaata gtacgcagtt
      301 ttgtgtcgct tatagccata aataatattt ggtacgctca gaaataatgg tttggtacac
      361 atctggtaca caatatcaca tggacaacag cgcacagtaa acaactatat ttgacgttcg
      421 tagacatact caggtgaaaa aagggttgtt tttcgagtta taacagacag ttaattaact
      481 acagtggaca atgctagaca atgacagaca cagataagca acctaccttc ctctttcgcg
      541 accacgttat ctttggcgat attcattccc gcgcgcagat caaacgcggt gatcacgcca
      601 tcaatgggcg ctttgagcgt aaagcgagtc tggatttttt gcgtggcgat caggtggcga
      661 atatccgcct ccggcattcc cgccagtcgc agccgctcaa gaatgccttc agtctgggtc
      721 gccgtaccgc cggtttcgcg caacagtaaa tactcactct gcgcttccac ccagtcggga
      781 atggtcaggt cgagaagcgg tgtgcccttt tgtactttat cgcccacggt aagcggatac
      841 accttgtcga taaacccggc agcgcgagcc tgcacaatgg catactgata ctcgttgtaa
      901 ctgacattcg ccgggaaact ctgggcaaaa gtcagcggcc cgcgcgtgac ggtagccgtt
      961 ttcaccccca gattctgcgt ctgagtcggg tcaatgcgca caccagacgc agaactctct
     1021 tcatcggcat atttcggcac cagatccata tccataaacg gcgatttacc tggtttatcg
     1081 aaccgcgtat tgggatacat cgggtcgtac cagaataaga ttttacgttc tgcggtcggc
     1141 attttttctg tgtgcggttc cgcctggtca aaccaggtaa aacctgccgc tgaaataata
     1201 ccgcccgcga tcatgctgcc gataataagc gcgatttttt tcatctcatt aaacctgggt
     1261 tactggctga ctttaatatc ctgtaataaa gaaaggttgc cctgctggac aaaattaaac
     1321 gccactttgt cgccggtttt aatttcactc attttcgtct gcggggtgat ggtaaagcgc
     1381 atggtcatct ccggccagtt gacggcagca atcggatcgt gatggatggt gatttttttg
     1441 ctttccagat caacaccttt taccacaccg gtggcactaa tgacctgtgg ttgtgcttcg
     1501 ctcatggttt catgatgatg ttcgttagcc tgggcattaa agccaataac ggtaaacaga
     1561 ctgaacattg cgacttgcag tgcttttttc atttattctc tcctggagtt aaaaattatt
     1621 gctgccaacc gccccctaag gcggtataca aataaatttc gttaacctga cgggcataat
     1681 tcaggtcaag taaagtttgt cgggttgcaa ataaagaacg ctcggcatcc agcacttcca
     1741 gataacttac cgcgccgtgc tgatataatg cccgcgcccg ttgcagggta atttgcagcg
     1801 acgccagata acgctgctgg gcgctgattt gatcgtccag gctttgacgt aatgccagcg
     1861 catctgccac ctctttaaag gcgttctgga ttttctgttc ataattcacc accgactgct
     1921 gctggcgaat ttcggcgata tccagattgg cctggttgcg tccggcatta aaaatgggga
     1981 tctcaatttt aggaataaaa ttccacatcc cgctgctagc gttaaataat gacgacagat
     2041 cgctgctggc ggttgatatt ccgctggtca ggctgataga cgggaaaaat gccgcacgcg
     2101 cagcaccaat attagcatta gccgccatta acgcgtgttc agcttccata atatccgggc
     2161 gctgcaataa gatttgcgac gacaagcccg ccggtaattt aacgctttgc aggctgccgc
     2221 tgtttactgt ctgcgcttgc ggtagcttgc cgtagcttcc caataacagt tgcaatgcat
     2281 tattcgcctg cgccagttcc ccctgacgtt tagcgatgtc actgcgggta ctttctatca
     2341 ccccgcgagc ctgttccagc gccagaacat tgctgctacc ggtcaacagt tgtttttcga
     2401 caaacgcata tgactgctga taattacgca gcgtttcttc ggctatttgc aattgcgcat
     2461 acgccagctg ctgattgaaa tagctttgcg cgacattaga aaccagcaaa atatgcaccg
     2521 cgcgctgggc ttcctcagtg gctaaataat tttggcgctc ggcttcgctc atgttcttta
     2581 agcgaccgaa aaaatcgaga tcaaagctgg cgttaaggcc agtcgagaac tcccgcgtcg
     2641 tggctgaatc gcctttaaga ttgccgctcc agctgccgct gccctagcca ttgagctgtg
     2701 ggtagcggtc ggcatcggtc agacgatatt gcgcccgtgc ctcctgcact ttcagcgtcg
     2761 ccatgcgcaa atcccggtta ttcaccagcg cctcgctaat cagcgtcttc acctgattat
     2821 caacaaaaaa ggtgcgccag cccacgttct ggtagttatc cgctgcgtta accaggccgt
     2881 tctggctgag tgagaactgc tgcggtacgg gcattaccgg acgctgataa tccggtgcca
     2941 gtgaacaacc agttagcgca agggccacac aaaatggcag aagtttacaa ggagacatag
     3001 gctcataatt tctggtgatt ttatgtcgcc aactttactc gccaggctct gattttccgg
     3061 tgacaggaaa atgacaaaat tgtcattttg ccaataagcg attgccatct gatcccgcta
     3121 ctctagaatt gcccgggcaa catgcggagg aaatatgaaa ctgttgattg tcgaagatga
     3181 aaagaaaacc ggagaatact tgaccaaagg gttaaccgaa gccggttttg tggtcgattt
     3241 ggccgacaac gggctgaatg gctaccatct ggcgatgacc ggtgattatg acctgataat
     3301 cctcgatatt atgctgccag acgtgaacgg ctgggatatc gtgcgcatgt tgcgctccgc
     3361 caataaaggg atgccgattc tgttgcttac cgcgcttggc accattgaac atcgcgtcaa
     3421 ggggctggag ttgggggcag atgactacct ggtgaaaccg ttcgcttttg ctgaactgct
     3481 ggcgcgggtg cgcactctcc tgcggcgcgg ggcggcggtg attatcgaaa gtcagtttca
     3541 ggttgccgat ctgatggtcg atctcgtcag ccgcaaagtc accctcagcg gcacgcgcat
     3601 cactctgacc agtaaagaat ttactctgct ggagtttttt ctccgccatc agggcgaagt
     3661 gctgccccgc tcgcttatcg cctcgcaggt atgggacatg aatttcgaca gcgacactaa
     3721 cgccattgat gtggcggtga agcggctacg cggcaaaatc gacaacgact ttgagccgaa
     3781 gctgattcaa accgtgcgcg gcgtgggtta catgcttgag gtgccggatg gtcagtaagc
     3841 catttcagcg cccgttttcg ctggcaaccc gcctgacctt ttttatcagc ctggccacca
     3901 tcgcggcgtt tttcgccttt gcatggatca taatccactc agtaaaagtg cattttgccg
     3961 agcaggacat taatgattta aaagagatta gcgccaccct tgaacgggta ctaaatcacc
     4021 ctgacgaaac gcaagcccga cgcttaatga cgctggaaga tatcgtcagt ggttattcca
     4081 acgtgttgat ttccctggca gatagccacg gcaaaacggt gtatcactcc cccggtgcgc
     4141 ctgatatccg cgagtttgca cgtgatgcca tacccgataa agacgcgcgg ggtggcgagg
     4201 tgtatctcct ttccggcccg acgattatga tgccaggcca cggtcacggg catatggaac
     4261 acagcaactg gcggatgatt aacttgccgg ttggcccgtt ggtggacggc aaaccgattt
     4321 atacgctcta catcgcgctg tcgatcgatt ttcatcttca ttacataaat gatttgatga
     4381 ataaacttat tatgaccgca tcggtaatca gcatcctgat cgtctttatc gtgctgttgg
     4441 cggtacataa aggtcacgcg ccgatccgca gcgtcagccg tcaaatccag aatatcacct
     4501 cgaaagatct cgacgttcgc cttgacccgc agaccgtgcc gattgagctg gaacagctgg
     4561 tactgtcgtt caaccatatg atcgagcgta ttgaggatgt ctttactcgc cagtccaatt
     4621 tctcagcgga tatcgcccac gaaattcgca caccaattac taatctcatt acgcaaacgg
     4681 aaatcgccct cagccagtcg cgcagccaga aggagctgga agatgtgctc tactctaatc
     4741 tcgaagagct gacgcgaatg gcgaaaatgg tcagcgatat gctgtttctc gctcaggccg
     4801 ataacaacca gctaatcccc gaaaagaaaa tgctcaacct ggcggatgaa gtcggcaaag
     4861 tgttcgattt tttcgaggcg ctagcggaag atcgcggcgt ggagttgcgg tttgttggcg
     4921 acaaacgtca ggttgcgggc gatccgttga tgctgcgtcg ggcgctaagc aacctgcttt
     4981 ctaatgccct tcgttatacg ccaaccagag agacaattgt agtacgctgt cagacggtcg
     5041 atcaccaggt gcaagtttcc gtcgaaaacc ccggtacgcc cattgcgccc gagcacttac
     5101 cgcgattgtt tgaccgtttc tatcgcgttg acccctcccg ccagcgaaaa ggtgaaggta
     5161 gcggtattgg gctggcgata gtgaaatcga ttgttgtcgc gcataaaggc acggttgcgg
     5221 taacgtcaga tgcgcggggg acaaggtttg ttatcacatt acccgcttaa ttattcagca
     5281 aacggcaact tttataacca gtgtaaaaat aacgtgccgc aatagtcata catattaatg
     5341 gtaaaaagct gtcacaattc ataaaaaacc ttaatatacg ccaccctaaa cataaccagc
     5401 gttaatgtaa ggtttttgtg tggactggct tcttgatgtt tttgctacct ggctctacgg
     5461 cttaaaagta atcgcgataa cgttagcggt catcatgttc atcagcgggc tggacgattt
     5521 ttttattgat gtcgtctact gggtacgccg cattaaacgc aagttgagtg tttatcgccg
     5581 ctacccgcga atgagttacc gcgaactgta taaaccagat gaaaaaccgt tagcgattat
     5641 ggttccggcg tggaatgaaa cgggcgtcat cggcaatatg gccgagctgg cggcgaccac
     5701 gctcgactac gaaaactatc atatctttgt tggcacctac cccaacgacc ccgatactca
     5761 acgtgatgtt gacgaagtgt gcgctcgctt tccgaacgtg cataaggtag tctgcgcgcg
     5821 tcctggtccc accagcaaag ccgactgcct gaacaacgtg ctggacgcca tcacccagtt
     5881 tgaacgtagc gccaatttcg cttttgctgg ttttattctg catgacgccg aagatgtgat
     5941 ttcaccgatg gaattgcgtc tgttcaacta tctggtcgag cgtaaagatc tgattcagat
     6001 cccagtgtat ccgtttgaac gcgaatggac gcacttcacc agcatgactt acattgatga
     6061 gttttcagaa ctgcatggca aagatgttcc ggtgcgtgaa gccctcgccg gacaagtgcc
     6121 cagcgcaggc gtcggcacct gtttcagccg ccgcgccgtg accgcactgt tagctgacgg
     6181 taacggtatt gctttcgacg tgcagagtct tactgaagat tacgacattg gcttccgcct
     6241 gaaagaaaaa ggtatgacgg aaatttttgt ccgttttccg gtggtggacg aagccaaaga
     6301 acgcgagcag cgtaaatttt tacagcacgc acggacgtca aacatgatct gcgtgcgcga
     6361 atatttcccc gatacctttt cgactgcggt tcgacaaaaa tctcgctgga tcatcggcat
     6421 tgttttccag ggctttaaaa cccataaatg gacctccagc ctgacgctga actactttct
     6481 ctggcgcgac cgcaaagggg caatcagtaa ctttgtcagc ttcctcgcga tgctggtgat
     6541 gatccagctt ttgctgttgc tggcgtatga aagtttgtgg cccgatgcct agcatttcct
     6601 ttctattttt agcggcagcg catggttaat gaccctgctg tggctaaact ttggcttgat
     6661 ggttaaccgc atcgtgcagc gagtgatttt cgtcactggc tactacggcc tcacgcaggg
     6721 cctgctttcc gtcctgcgtc ttttctgggg caacctgatt aactttatgg ctaactggcg
     6781 cgtgctgaaa caggttcttc agcacggcga tccgcgtcgc gtcgcgtggg ataaaacaac
     6841 gcatgacttc cccagcgtca ctggcgatac ccgctcgttg cgcccgttag gtcaaattct
     6901 gctggaaaat caggtcatca ctgaagaaca actcgataca gcactgcgta atcgcgtcga
     6961 aggtctacgc ctgggcggtt caatgctgat gcaggggctg attagcgccg agcagctggc
     7021 acaggcgctg gcagagcaaa acggcgtggc gtgggaatcc atcgatgcct ggcagatccc
     7081 gtcctcgctg attgccgaaa tgccagcctc cgtagcgctg cattatgcag tactgccgct
     7141 gcgtctggaa aatgatgagt taattgtcgg cagtgaagat ggtattgacc cggtttcgct
     7201 ggcggccctg acgcgtaaag tcggacgcaa agtgcgttac gtcattgttc tgcggggaca
     7261 aattgtcaca gggttacgcc actggtatgc acgccgacgc ggtcacgatc cgcgggcaat
     7321 gttgtacaat gcggttcagc atcagtggct cacggaacag cagaccggtg aaatctggcg
     7381 gcaatatgtg ccgcatcagt tcctgttcgc cgaaatactg accacgctcg gtcatattaa
     7441 tcgttcagca attaacgtgt tgttattgcg ccatgaacgc agttctctgc cgctcggcaa
     7501 gtttttggtc accgaaggcg ttatcagcca ggaaacgttg gatcgcgtcc tgacaattca
     7561 acgcgaatta caagtttcga tgcaatcact attactcaaa gcaggtttaa acacagaaca
     7621 ggttgcgcaa ctggagtccg aaaatgaagg agaataacct taatcgcgtc atcggatggt
     7681 ctggtttact gctgacgtct ttattgagta ccagcgcact cgcagacaat atcggcacca
     7741 gcgcagaaga gctggggctg agcgattatc gccattttgt tatttatccc cgtctcgata
     7801 aggcgctgaa ggcacagaaa aataacgacg aagcaaccgc catccgcgaa tttgaatata
     7861 tacaccaaca ggtgccggat aatattccgc tgactttata ccttgcggaa gcctatcgtc
     7921 attttggtca tgatgaccgg gcgcggctgt tgcttgagga tcaactgaaa cgtcacccag
     7981 gagatgcccg acttgagcgc agtctggcgg ctattccagt tgaagtgaaa agcgttacga
     8041 ctgttgaaga actgcttgcc cagcaaaaag cgggcgacgc tgcgccgacc ctgcgttgtc
     8101 gcagtgaagt cgggcagaat accctgcggc tggcacaatt acctgtcgcc agagcgcaac
     8161 tgaacgatgc gacgtttgct gcatcgccgg aaggaaaaac gctgcgaacc gatctgctgc
     8221 aacgggcaat ctacctgaaa caatgatccc aggcagatac gctatacaat gaagcacgcc
     8281 agcagaacac attaagcgcg gcagaacgcc gtcagtggtt tgacgtgctt cttgccgggc
     8341 agctggacga tcggatcctg gcactgcaat cacaggggat cttcactgat cctcagtcat
     8401 atattactta cgcgaccgcg ctggcttatc gtggcgaaaa agcacgcctc cagcattatc
     8461 tcattgaaaa taagccgctg tttaccacgg gcgcacaaga gaaaagttgg ctctatctgt
     8521 tatctaaata cagcgccaac cccgttcagg cattggcgaa ttatacggtg cagtttgccg
     8581 ataaccgcca gtatgttgtt ggcgcgacgc taccggtgct gttaaaagaa ggtcagtacg
     8641 acgcagcgca aaaactgctc gccaccctcc ccgccaatga aatgcttgag gagcgttatg
     8701 ctgtcagcgt ggcgacccgt aacaaggctg aatctctgcg tctggcacga ttgctgtatc
     8761 agcaagaacc gacaaatctt ccccgcctgg atcaactgac ctggcaactg atgcagaacg
     8821 agcagtcacg cgaagctgcc gatttattgc tgcaacgcta tcctttccag ggcgatgcgc
     8881 gtgtcagcca gactttaatg gcgcgactgg cgtctctgct ggaaagtcat ccttacctag
     8941 caacgccggc gaaggtggcg attttatcga aacccttacc gctggcggag caacgtcagt
     9001 ggcaaagtca gttgccgggt attgcagata attgcccggc aatagttcgc ttgctgggcg
     9061 atatgtcgcc ttcctacgat gctgccgcct ggaaccgtct ggcaaagtgt tatcgggaca
     9121 cgctacccgg tgtggcgttg tatgcatggc ttcaggccga acaacgacaa ccgagcgcct
     9181 ggcaacatcg tgcggtagcc tatcaggcgt atcaggttga ggactacgcc accgcactgg
     9241 cggcctggca gaaaatcagt cttcacgaca tgagtaatga tgatctgctc gccgccgcca
     9301 acaccgctca ggcagcggga aatggcgcgg ctcgcgatcg ctggcttcaa caggcagaac
     9361 aacgtgagct gggaaacaat gctcaatact ggtggctgca tgcgcgacgt tatatcccag
     9421 ctcagccaga actcgcactg aacgatctca cgcgctcaat caatattgcc ccttctgcca
     9481 actcttacgt tgcgcgggcg acaatttatc gccaacgtca taatgttccg gccgcggtga
     9541 gtgatttgcg cgccgcgctg gaactggaac caaataatag caacacccag gcagcgcttg
     9601 gttacgcctt gtgggatagc ggtgatatcg cacagtcgcg ggaaatgctc gaacaggcgc
     9661 ataaagggct tccggacgat ccggcactga tccgacaact ggcctacgtg aaccagcgtc
     9721 tggatgacat gcctgcgacg cagcactacg cccggctcgt gattgatgac attgataatc
     9781 aggcataacc ccactgaccc cagagcaaaa tcagcaacgc ttcaatttcc gccgtttgca
     9841 tgaggaggtc ggtcgccgct ggacgttcag tttcgattct tccatcggct tgcgttccgg
     9901 cgcaatgagt accgctaaca ataatgtcgg cggtgcagcg cctgggaaaa gctatcgtag
     9961 ctacggacaa ctggaagccg agtaccgcat cggacgcaat atgctgctgg aaggcgacct
    10021 gctctcagtt tatagccgcg tctttgccga taccggagaa aacggggtga tgatgccggt
    10081 gaaaaatccg atgtccggca ccggtctgcg ctggaagccg ctgcgcgatc agatcttttt
    10141 cctcgccgtc gaacagcagt tgccgctgaa cgggcaaaat ggcgcatccg ataccatgct
    10201 gcgcgccagc gcctcattct ttaatggcgg caaatacagc gacgaatggc acccgaacgg
    10261 ttcaggctgg tttgcccaaa acctgtacct cgatgcggcg caatatatcc gccaggatat
    10321 tcaggcgtgg acggcagatt atcgcgtcag ctggcatcag aaggtagcta acggacagac
    10381 tattgagcct tacgctcacg ttcaggacaa cggctatcgt gataaaggca ctcagggcgc
    10441 gcagcttggc ggtgtcgggg tccgctggaa tatttggacc ggcgagacgc actacgacgc
    10501 ctggccgcac aaagtcagtc tcggcgtcga gtatcaacat acctttaagg cgattaatca
    10561 acgtaacgga gagcgcaaca acgcgtttct caccattgga gtgcactggt aaatgcgtaa
    10621 gttcattttc gtattgctga cactgctttt ggtcagccct ttttcctttg cgatgaaagg
    10681 tattatctgg caaccacaaa accgagatag tcaggttacc gatacccagt ggcaggggct
    10741 gatgagtcag ttacgttttc aaggcttcga taccctggtt ttgcaatgga cccgttacgg
    10801 cgatgcattt acccagccag aacagcgcac gttattgttt aagcgtgccg cagctgcgca
    10861 acagactggc ctgaagctga ttgtcgggct gaacgccgat ccggaatttt ttatgcacca
    10921 gaaacagtcg tctgcagcgc tggaaagcta tcttaatcgc ctgctggctg ccgatctcca
    10981 gcaagccaga ttatggagcg ccgcgcctgg cataacgccg gatggctggt acatcagcgc
    11041 ggaaattgac gacctgaact ggcgcagcga agccgcccgt caacctttgc taacatggtt
    11101 aaacaacgcg cagcggctga ttagcgatgt ttcagcaaaa ccggtttata tcagtagttt
    11161 tttcgccgga aacatgtcgc ccgatggcta tcgccaactg ctggaacaag ttaaagcaac
    11221 cggagttaat gtctgggtac aggatggcag cggcgtggat aaacttaccg ctgaacagcg
    11281 tgaacgttat ctacaggcca gcgccgattg ccaaagctcc gccccagcaa gcggcattgt
    11341 ttatgaactg tttgtcgccg gcaaaggcaa aacctttaca gcgaaaccga aaccggacgc
    11401 agaaattgcc tcgctgttag cgaaacgttc ctcttgcggt aaagacatgc tctatttctc
    11461 tctgcgctat ttgcccgtcg cgcacggcat tctcgagtat taaatcccct ccaggtaagt
    11521 cgggtacgac ctggcttacc tctttcgctc tcgtcagaat tccatcaaga ttaattgcta
    11581 aaaagcatgc taaagaaaga agatcgttat cggcgaacgg gccgatcact catgcaggaa
    11641 gcatagatcc tttgagcgga gtttctgata tctgaggagt gcgaaatgca attgagcagc
    11701 agtgaacctt gcgtggtgat cctgaccgaa aaagaggtag aggtaagcgt caataaccat
    11761 gctacgttta cccttccgaa aaactacctg gccgccttcg cgtgcaacaa taacgtcatt
    11821 gaactctcaa cgttaaatca cgtattaatc acccacatca accgtaacat catcaacgat
    11881 tatctgttgt ttttaaataa gaaattaacc tgtgtaaagc cctggtcgcg gctggcaacc
    11941 ccggttatcg cttgtcatag ccgtacaccg gaagtgttcc ggctagccac caaccacagc
    12001 aagcagcaat ccagcaaacc ctgcgaagcg gagttgacgc gcgcattact ttttaccgta
    12061 ttgtctaact ttcttgagca atcgcggttt attgccctac tgatgtatat cttacgcagc
    12121 agcgtccgcg acagcgtctg ccgcattatt caaagcgata ttcagcatta ctggaatctg
    12181 cgaattgtcg ccagttcgct atgtttaagc cccagcctgc tcaaaaagaa attaaaaaac
    12241 gaaaatacca gctatagcca gattgtcaca gagtgtcgta tgcgttacgc cgtacagatg
    12301 ttattgatgg ataacaaaaa tatcactcag gtggcgcaat tatgtggcta tagcagcacg
    12361 tcgtacttta tctctgtttt taaggcgttt tacagcctga caccgttgaa ttatctcgcc
    12421 aaacagcgag aaaaagtgat gtggtgaagg gcaaagcgga aacggataag acgggcataa
    12481 atgaagaaga aatggcgcgc cctgcaggat tcgaacctgc ggcccacgac ttagaaggtc
    12541 gttgctctat ccaactgagc taagggcgcg ttgataccgc aatgcggtgt aatcgcgtga
    12601 attatacggt caacccttgc tgagtcaatg gcttttgatc tggttgctga acaagtgaac
    12661 gaccgcgtct gattttctga tttatttcgc tatagcggca aacaaacgca caccgctgcg
    12721 cgtctgaatc aagaaaaccc gtattttcat gtatcaaagt acaatttacc gccctaacgg
    12781 aaaattgtcc gctcctatga gactggtaac tatgaaacca acgtcggtga tcattatgga
    12841 tactcatcct atcatcagaa tgtctattga agttctgttg caaaaaaaca gtgaattgca
    12901 gattgtcctg aaaacggatg attaccgcat aaccatcgat tatctccgaa cccgtcctgt
    12961 tgatttaatc attatggata tagacttgcc cggaacagac ggttttacct tcctg
//
LOCUS       DAEEUE010000104        12907 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.104, whole genome shotgun sequence.
ACCESSION   DAEEUE010000104 DAEEUE010000000
VERSION     DAEEUE010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12907
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.104"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="KLK84_003277"
     CDS             complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="KLK84_003277"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBE2010492.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1806..3224)
                     /locus_tag="KLK84_003278"
     CDS             complement(1806..3224)
                     /locus_tag="KLK84_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBE2010493.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3378..3722)
                     /locus_tag="KLK84_003279"
     CDS             complement(3378..3722)
                     /locus_tag="KLK84_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HBE2010494.1"
                     /translation="MQYLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="KLK84_003280"
     CDS             complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="KLK84_003280"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HBE2010495.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4712..5647)
                     /locus_tag="KLK84_003281"
     CDS             complement(4712..5647)
                     /locus_tag="KLK84_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HBE2010496.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5727..6842
                     /gene="yneK"
                     /locus_tag="KLK84_003282"
     CDS             5727..6842
                     /gene="yneK"
                     /locus_tag="KLK84_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HBE2010497.1"
                     /translation="MVTPVSISNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6992..8182
                     /gene="ydeA"
                     /locus_tag="KLK84_003283"
     CDS             6992..8182
                     /gene="ydeA"
                     /locus_tag="KLK84_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HBE2010498.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8207..8872)
                     /gene="marC"
                     /locus_tag="KLK84_003284"
     CDS             complement(8207..8872)
                     /gene="marC"
                     /locus_tag="KLK84_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HBE2010499.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9084..9518
                     /gene="marR"
                     /locus_tag="KLK84_003285"
     CDS             9084..9518
                     /gene="marR"
                     /locus_tag="KLK84_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HBE2010500.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAEICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9538..9921
                     /gene="marA"
                     /locus_tag="KLK84_003286"
     CDS             9538..9921
                     /gene="marA"
                     /locus_tag="KLK84_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HBE2010501.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9953..10171
                     /gene="marB"
                     /locus_tag="KLK84_003287"
     CDS             9953..10171
                     /gene="marB"
                     /locus_tag="KLK84_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HBE2010502.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="KLK84_003288"
     CDS             complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="KLK84_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HBE2010503.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLIFDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11365..12483
                     /gene="ydeE"
                     /locus_tag="KLK84_003289"
     CDS             11365..12483
                     /gene="ydeE"
                     /locus_tag="KLK84_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HBE2010504.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            12610..12705
                     /gene="mgtS"
                     /locus_tag="KLK84_003290"
     CDS             12610..12705
                     /gene="mgtS"
                     /locus_tag="KLK84_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HBE2010505.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         3400 a         3296 c         2951 g         3260 t
ORIGIN      
        1 gtcaagctta caacattaac aggcgctgca tgtgacacct gttaatgata aagggatata
       61 ggatgttgta actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac
      121 ggtgttatta gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca
      181 ggtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
      241 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
      301 ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
      361 ctccgttacg ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
      421 ccggaagtgt gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga
      481 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
      541 aacgcccggt gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg
      601 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
      661 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
      721 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
      781 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
      841 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
      901 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
      961 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1021 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga
     1081 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     1141 tcagttttgc cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     1201 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     1261 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     1321 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
     1381 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     1441 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     1501 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     1561 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc tgtttgaatt
     1621 ggtgcgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     1681 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     1741 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     1801 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     1861 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc
     1921 gttcggtacg atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt
     1981 tgttgccagg tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga
     2041 ccatcgacag gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc
     2101 accagcccct tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta
     2161 tcgccacatt catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc
     2221 atgaccgtca gatgttgtgt ctgtttcagc gatggacttt tcagcgcctc ataaagaccg
     2281 gaccgtgagt aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt
     2341 agcaaattga tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca
     2401 ataccgagac gtgcggagaa catttccgga atggagaacg gcgaaccgac cgagatatca
     2461 atcaccgaat ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat
     2521 gttacctgcg gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga
     2581 ctccccgctc cgccaatgac taccgaggca ataaccgaca caataagcgc gatggcgggc
     2641 atcatctgtt ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa
     2701 ggcacgatca acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag
     2761 aaatccagac tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg
     2821 gcaatcagac agtaattaaa tagccgtaaa gcacttactg gctcatactt atttttcccc
     2881 agacgcttat cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa
     2941 ttgataacca gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc
     3001 acataactga tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc
     3061 gccattacgc cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca
     3121 ccaataaaat agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga
     3181 ccttcctgga acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata
     3241 caattgcggg tggcaattat ctcaacgtga taagtaaatt ctcataaagg atagcatgtt
     3301 cgctaacagc ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat
     3361 tctcatggct gattcatcta tacaaccagc cagtgataga ttaccgccac gatataacgt
     3421 tgttgttctt cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg
     3481 cgacagcagg tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt
     3541 tgcttaccgt cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca
     3601 tgttgttcga tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga
     3661 cgaaagcgag agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagatactgc
     3721 attaataaac cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa
     3781 gcgagttacc tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa
     3841 caatcgccac aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa
     3901 actcccccgc gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct
     3961 gcattggcgt caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga
     4021 caaacgtccg ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt
     4081 gcagaacggt tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag
     4141 ccgcatttcg cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat
     4201 cagacacacc gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc
     4261 gcccttgcaa catatcgcag accaccagcg caccggtatt aatgaacgga ttacgcggaa
     4321 taccctgttc catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc
     4381 cgacgcgttg ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca
     4441 ctttggaaat agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc
     4501 cgtcaacggt acagatagca atccccaatc gggaaccgtc tactgtagcc agcgccggaa
     4561 tataatccgc gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta
     4621 aaattgcatt atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac
     4681 ctgcgagtat atcagagctg aatatgtcgc gtcagatccg gtctttccac actgtctgga
     4741 tattacagaa ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca
     4801 cgccaccaaa ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc
     4861 attccagacg tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg
     4921 aaaggccgaa ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg
     4981 tgattgcagc aacggggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat
     5041 tcgccagaac cgttggcgga tagtagttac ctgctccagc catcttttcc ccgcccagta
     5101 acaaacgcgc accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat
     5161 caaaacgagc cattggtccg agagcgttct cttcatcacg gggatcgccc attttcaagg
     5221 ctgccgcagc tgacacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa
     5281 agcgttttgc cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt
     5341 taaccgccag ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt
     5401 ccagtacgca ttttttcagt gccgctccag cacgaacact tccggtcacc gtgacagcag
     5461 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     5521 atacgccttg cgggattccc gcatctttaa acacttgggc aatgagctga gcacagccca
     5581 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     5641 cacgcatcac ctgcctttga tagttcttgc agctcaaatg ccctatatag tgatgttccg
     5701 atgacttatg actatatggg gcaaatatgg ttacgccagt aagcatcagc aattacatat
     5761 ctcttcctga tgattttccc gctcgtaata ttgcacctca agtaaaagaa gttttaaagg
     5821 attttattga tgcactcagt accataatat gtgatgaaga gtggcgcacg tctttaaaca
     5881 ccaactcagc cacaaaaaag atatttaaca accttgataa cttatcatat attcagagga
     5941 cttcttttcg gggtaacgac acactataca atgaaaaggt tcagtttaaa ctcacttatc
     6001 ctgtcaagaa tggaagacac aaagaaaata ttgaatttca ggtagtaata aatttaagcc
     6061 ctatttattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
     6121 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
     6181 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
     6241 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
     6301 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
     6361 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
     6421 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
     6481 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
     6541 tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat
     6601 tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag
     6661 catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg
     6721 taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct
     6781 cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt
     6841 aaaccgtgat gttaccgact ctctgactcg gaacagaatc agcgtcagag aaacggaaaa
     6901 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
     6961 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
     7021 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
     7081 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     7141 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
     7201 aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     7261 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     7321 tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc
     7381 tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat
     7441 ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt
     7501 cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     7561 actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     7621 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     7681 ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     7741 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
     7801 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact
     7861 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     7921 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     7981 taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc
     8041 gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc
     8101 ggtgcctgct tttgctgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     8161 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     8221 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     8281 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     8341 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     8401 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
     8461 tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat
     8521 tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
     8581 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     8641 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg
     8701 ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     8761 cactgttcat gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta
     8821 acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     8881 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     8941 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
     9001 gcatcggtca attcattcat ttgacttata cttgcctggg caatattacc ccctacaact
     9061 aattacttgc cagggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc
     9121 cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc
     9181 tgtctccgct ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg
     9241 cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc
     9301 gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata
     9361 agcgcggcgt actggtaaaa cttaccacca gcggcgcgga aatatgcgaa caatgccatc
     9421 aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg
     9481 caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg
     9541 tccagacgca atactgacgc tattaccatt catagcattt tggactggat cgaggacaac
     9601 ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatggcac
     9661 ctgcaacgga tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt
     9721 aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca
     9781 gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat
     9841 gttccgccgc ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca
     9901 ttaaatcatt acaacagcta gttgataacg tgacaacgtc actgaggcaa tcatgaaacc
     9961 actttcatcc gcaatagcag ctgcgcttat tctcttttcc gcgcagggcg ttgcggaaca
    10021 aaccacgcag ccagttgtta cttcttgtgc caatgtcgtg gttgttcccc catcgcagga
    10081 acaaccaccg tttgatttaa atcacatggg tactggcagt gataagtcgg atgcgctcgg
    10141 cgtgccctat tataatcaac acgctatgta gtttgttctg gccccgacat ctcggggctt
    10201 attaacttcc cacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc
    10261 cggtcataat gagcaccgca cctaaaaatt gcagacccgt taagcgttca tccaacaata
    10321 gtgccgcact tgccagtccg actacgggca ccagtaacga taacggtgca acccgccaag
    10381 tttcatagcg tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg
    10441 ccagatacat cagcgacaag atggtggtca tatcaatagt aaccagactg tgaatcatgg
    10501 atgcggaacc atcgaaaatc agcgagacaa caaagaaggg aatgattggg attaaagcgc
    10561 tccagattac cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga
    10621 agatgttgcc acacgcccaa ctaaatgctg cagccagggt caacataaag ccgagcatcg
    10681 ccacatgctg accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg
    10741 cgatccccgc caattgtttg ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga
    10801 tagtaaaaaa cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt
    10861 taatggcgca gaacagaaag gcaaactgcg caaaactgat ggttaatcca taccccaaga
    10921 gcaaattcag tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa gcgaccagca
    10981 taaagcgcaa accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca
    11041 caaaatttag cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca
    11101 ttctaccgcc tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct
    11161 ttaacagttg attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat
    11221 acttcggatt tttgccatgc gtccgatggt taataacaac gacgaggcaa gaagggcgct
    11281 ggtagagcgt cgtagggata agttcatttg tctgtccggc agtgcgtttc gttatgtttt
    11341 attttagaga tgtaaagaaa tagcatggct acgctgccat ttatgaccat ttacttgagt
    11401 cgccagtaca gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt
    11461 ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat
    11521 atgttactgg caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac
    11581 gtgacgctgg ttgtgctctt ttttgccctc attaactgcg cctattctgt ttttgctact
    11641 gtgctgaaag cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca
    11701 atcaactaca ccatgctaaa cattggctgg accatcggtc cgccgctcgg cacgctgttg
    11761 gtgatgcaga gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg
    11821 cttttcattc aaatatgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt
    11881 gtctggtcgc cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt
    11941 tttctggctt cttttgtaag cggcgcattt gcttcatgca tttcacaata tgtgatggtg
    12001 attgctgatg gggattttgc cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc
    12061 atggtggtta cgttgcaata ttccgtgggc cgccgactta acccagctaa catccgcgcg
    12121 ctgatgacag caggcaccct ctgtttcgtc atcggactgg tcggttttat tttttccggc
    12181 aacaacctgc tattgtgggg tatgtcagct gcggtattta ctgtcggtga aatcatttat
    12241 gcgccgggcg agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat
    12301 ttttccgccc agtctttagg ctggcttggt gccgcgatta acccattagt gagtggcgta
    12361 gtgctaacca gcctgccgcc ttcctcgctg tttgtcatct tagcgttggt gatcattgct
    12421 gcgtgggtgc tgatgttaaa agggattcgt gcaagaccgt gggggcagcc cgcgctttgt
    12481 tgatttaagt cgaacacaat aaaaatttaa ttcagccttc gtttaggtta tctctgctaa
    12541 tatctttctc attgagatga aaattaaggt aagcgaggaa acacaccaca ccataaacgg
    12601 aggcaaataa tgctgggtaa tatgaatgtt tttatggccg tactgggaat aattttattt
    12661 tctggttttc tggccgcgta tttcagccac aaatgggatg actaatgaac ggagataatc
    12721 cctcacctaa ccggcccctt gttacagttg tgtacaaggg gcctgatttt tatgacggcg
    12781 aaaaaaaccg ccagtaaacc ggcggtgaat gcttgcatgg atagatttgt gttttgcttt
    12841 tacgctaaca ggcattttcc tgcactgata acggatcgtt gacacagtag catcagtttt
    12901 ctcaatg
//
LOCUS       DAEEUE010000105        12801 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.105, whole genome shotgun sequence.
ACCESSION   DAEEUE010000105 DAEEUE010000000
VERSION     DAEEUE010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12801
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.105"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..351)
                     /locus_tag="KLK84_003291"
     CDS             complement(<1..351)
                     /locus_tag="KLK84_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBE2010506.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKAVIEQHMSLNQAAAHFMLAGSGSVARWLK
                     VYEERGEAGLRALKI"
     gene            727..2367
                     /gene="trg"
                     /locus_tag="KLK84_003292"
     CDS             727..2367
                     /gene="trg"
                     /locus_tag="KLK84_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HBE2010507.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2405..3328)
                     /locus_tag="KLK84_003293"
     CDS             complement(2405..3328)
                     /locus_tag="KLK84_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE2010508.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3545..4888
                     /locus_tag="KLK84_003294"
     CDS             3545..4888
                     /locus_tag="KLK84_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBE2010509.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMTPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            5113..6768
                     /gene="opgD"
                     /locus_tag="KLK84_003295"
                     /gene_synonym="mdoD"
     CDS             5113..6768
                     /gene="opgD"
                     /locus_tag="KLK84_003295"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HBE2010510.1"
                     /translation="MDRRRFIKGLMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6908..7132
                     /locus_tag="KLK84_003296"
     CDS             6908..7132
                     /locus_tag="KLK84_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HBE2010511.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            7195..7731
                     /gene="rimL"
                     /locus_tag="KLK84_003297"
     CDS             7195..7731
                     /gene="rimL"
                     /locus_tag="KLK84_003297"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HBE2010512.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7726..8706)
                     /locus_tag="KLK84_003298"
     CDS             complement(7726..8706)
                     /locus_tag="KLK84_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010513.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8830..9822
                     /gene="tehA"
                     /locus_tag="KLK84_003299"
     CDS             8830..9822
                     /gene="tehA"
                     /locus_tag="KLK84_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HBE2010514.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9819..10412
                     /gene="tehB"
                     /locus_tag="KLK84_003300"
     CDS             9819..10412
                     /gene="tehB"
                     /locus_tag="KLK84_003300"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HBE2010515.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10715..11383
                     /locus_tag="KLK84_003301"
     CDS             10715..11383
                     /locus_tag="KLK84_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HBE2010516.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(11401..11907)
                     /gene="tnpA"
                     /locus_tag="KLK84_003302"
                     /pseudo
     CDS             complement(11401..11907)
                     /gene="tnpA"
                     /locus_tag="KLK84_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            11915..12801
                     /locus_tag="KLK84_003303"
                     /pseudo
     CDS             11915..12801
                     /locus_tag="KLK84_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3335 a         3004 c         3202 g         3260 t
ORIGIN      
        1 aatcttgagc gcgcgtaaac cagcttctcc gcgctcttca tagaccttca gccacctggc
       61 tacagaacca ctaccagcaa gcataaagtg agcagcagcc tgattaaggg acatgtgctg
      121 ctcgatcaca gctttcacga ccttaatacg caactctgga tcagcactaa cgcctttagg
      181 tttgggaatt aaaccttttt ctccatgttt ttcatagagg gcaacccatg tcctgacctg
      241 ggttcggggg acaccaaaac gtgccgagat gatcctgtaa ccatcatcag tagtgaagta
      301 gtgattcacg acttcaaggc gcttttcaaa agggtatttt ggctttgaca tattaggggc
      361 tattccattt catcgtccaa caaaatgggt gcagtacagt tgatagaaat atcggtcggg
      421 gccttcgtct ttctgattcc cggttagcct gaaaacagaa agtctcaggc acccgcaggc
      481 atcctatgag gtttccttag ggacgaaaat aatcacttca cgaaattgcg tgctgttttc
      541 cagaattttt cgtcattcgg gttagccagt ttagccattc gttactctct tcattccaat
      601 agcattaatt ttctctgcaa taattgttgt aaaaatgtga cgcaaagagg tttttggtca
      661 taagtaatta ccgtcaagtg ccgatgactt tctatcagga gtaaacctgg acgagagaca
      721 acggtaatga atacaactcc ctcacagcga ttaggttttt tgcatcacat caggctggtt
      781 ccgttatttg cctgcattct tggcggtatc ttagttctat tcgcattaag ttctgctctg
      841 gctggctatt tcctctggca ggccgatcgc gatcagcgtg atgttactgc ggagattgag
      901 attcggaccg ggttagcgaa cagttcagat tttttgcgtt cagcccggat caatatgatt
      961 caggccgggg ctgcgagtcg tattgcggaa atggaagcaa tgaagcgaaa tattgcgcaa
     1021 gccgaatcgg agattaaaca gtcgcagcaa ggttatcgtg cttatcagaa tcgaccggtg
     1081 aaaacacctg ctgatgaagc cttcgacact gaattaaatc aacgctttca ggcttatatc
     1141 acgggtatgc aacctatgtt gaaatatgcc aaaaatggca tgtttgaagc gattatcaat
     1201 catgaaagtg agcagatccg accgctggat aatgcttata ccgatatttt gaacaaagcc
     1261 gttaagatac gtagcaccag agccaaccaa ctggcggaac tggcccatca gcgcacccgc
     1321 ctgggtggga tgttcatgat tggcgcgttt gtgcttgccc tggtcatgac gctgataaca
     1381 tttatggtgc tacgtcggat cgtcattcgt ccactgcaac atgccgcaca acggattgaa
     1441 aaaatcgcca gtggcgatct gacgatgaat gatgaaccgg cgggtcgtaa tgaaatcggt
     1501 cgcttaagtc gtcatttaca gcaaatgcag cattcactgg ggatgacagt agggactgtt
     1561 cgacagggcg cggaagagat ttatcgtggc accagcgaaa tttcagctgg caatgcggac
     1621 ctgtcatctc gcaccgaaga acaagcggcg gctatcgaac aaactgccgc cagcatggag
     1681 caactcactg cgacggtgaa acagaatgcg gataacgcgc atcatgccag caaactggcg
     1741 caagaggctt ctattaaagc cagcgatggc gggcagacgg tttccggtgt agtaaaaacg
     1801 atgggcgcta tctccacgag ttcgaagaaa atttctgaga tcaccgccgt catcaacagt
     1861 attgctttcc agacgaatat tctggcactg aatgctgccg ttgaagccgc gcgagcgggt
     1921 gagcaagggc gtggatttgc cgttgtcgcc agcgaagtac ggacactcgc aagccgcagc
     1981 gctcaggcag cgaaagagat tgaaggcttg atcagtgaat cagtcaggtt aattgacctg
     2041 gggtcggatg aggtggcaac ggcagggaaa accatgagca ctattgttga tgccgtcgcg
     2101 agtgtcacac atatcatgca ggaaatcgcc gccgcctcgg atgaacaaag tagaggcata
     2161 actcaggtta gccaggcgat ttctgaaatg gataaggtga cgcaacagaa tgcttctctg
     2221 gtagaagagg cctcagcagc ggcggtgtcc cttgaagaac aggctgcacg attaactgag
     2281 gcggtgaacg tattccgtct gaacaaacat tctgtgtcgg cagaacctcg cggagcgggt
     2341 gaaccagtta gtttcgctac ggtgtgaaaa tgttcaagga gggatcgaca gatcccttca
     2401 cctttcagaa cggcattgat tttcgaatag cgttaatcat caactggcaa ccagaagaga
     2461 acgtcgcatc tacgcgggtc agtattccaa tcggttcgcc tgcaccatgt cccggaacag
     2521 gcagggccac cagcgtggca tgacgcaggt cgtcttttac agcgccagaa gggacaaacc
     2581 acacgtaatc gtattcaacg gtaagttgac gagatagcga agtagacagc gtttcgatac
     2641 aacccgaagg aattttacag ccctggctct gcactaatgc atctgaatgc tggcgtggcg
     2701 cagtgccttc tggtgataca acgaccggcc attccagcac ccggcttagc gttacgttct
     2761 cctgaagtag cgggtgatta gggcggacaa ccagcttcaa cgattcaaga aacagcagtt
     2821 cgtaattaag cccggtcatc agttcaggat ctgacatccg accaatgccg atatcgattt
     2881 ccccggtttt taaacccgcc agaatcatag ggttactcat tgtcgcaact tgcaaggtcg
     2941 tctctttttg ttgctgatga aactgaccta taaccgaagg taatatcccc agtgccgcag
     3001 taggtagtgc accaaccctg acgacatcat tattaagacc ttctttacga tgaagcgact
     3061 gtccggcagt gttgatggcg tcaagaactc tgactgcatg cgttaaaaat tgttcgccgg
     3121 gtaaggtaag ttgcgccccc tgacgaccac gctcaaacaa gcgagcgccc gtcagctgct
     3181 ccagttcatt caatgtatta gagagcgcag gttgactcaa attaagggtt tcagctgcgc
     3241 gccccaaagt tccttgttgt gcgacagcta cgaatgtatg aaggtggcgc aaacggatgc
     3301 gctgactaaa cagactattt ttttccataa gcgatgttaa aaacgaagcg gtgtcgctga
     3361 caagtgaagt tgtttgatta tgataacttg attgcaaaat attattaata attaaagcaa
     3421 ttatgttaca gcaaaatgga taatattgat gttttcgcgg cgagatcaca gtttgtaaat
     3481 tcttcccgcc agagtgaatg cggttaccta cactccagat tactgaccac tggaggcaga
     3541 cactatggcg aacagcatca cggcggatga gattcgggaa cagttttcgc aggcaatgtc
     3601 agccatgtac cagcaagaag ttccgcaata tggcacgctg ctggaactgg tagctgatgt
     3661 gaatctggct gtgctggaaa acaatcctca actgcacgaa aaaatggtaa atgcagacga
     3721 gctggcgcga ctgaatgttg aacgtcatgg ggcgattcgc gttgggactg cacaagagct
     3781 tgctactctt cggcggatgt ttgccattat ggggatgtac ccggtgagct attacgatct
     3841 ctcgcaggca ggggtgccgg tacattcgac agcatttcgg cccattgatg atgcttctct
     3901 ggcgcgtaat cccttccgcg tttttacatc cttactccgc cttgagctta tcgagaacga
     3961 aattttgcgc cagaaagcgg cggagattct gcgtcagcgc gatatcttca ccccacgttg
     4021 tcgacaactg ttagaggaat atgagcagca gggcggtttt aacgaaacac aggcacagga
     4081 gtttgtgcag gaagccctgg aaacgtttcg ctggcaccag ttagcaacgg tagatgaaga
     4141 aacctatcgc gccttgcaca acgaacatcg gttgattgct gatgtggtct gttttcctgg
     4201 atgccatatc aaccacctga cgccacgtac gctggatatt gaccgggtgc agtcgatgac
     4261 gcctgaatgc ggaattgaac ccaaaattct catcgagggg ccgccgcgcc gcgaggtacc
     4321 gattttacta cgccagacca gctttaaagc actggaagag acggtgttgt ttgcggggca
     4381 gaaacagggc acgcataccg cgcgctttgg tgaaattgag cagcgtggcg tggcattaac
     4441 gccgaaaggg cgacaactgt atgatgatct tctgcgtaac gctggaaccg ggcaggataa
     4501 tctcactcac caaatgcatt tacaggaaac cttccgcact tttcctgaca gtgagttttt
     4561 aatgcgtcag caagggttgg catggttccg gtaccgtctg acgccttcgg gtgaggcgca
     4621 tcgtcaggcg attcatcctg gagacgatcc acagccctta attgaacgtg gttgggtagt
     4681 ggcgcaaccc atcacctatg aagatttctt gcccgttagc gcggcgggga tcttccagtc
     4741 aaatctgggt aatgaaacgc agacacgcag tcacggtaat gccagtcgcg aagcatttga
     4801 gcaggcgttg ggttgtccgg ttttggatga gttccagctt taccaggaag cggaagaacg
     4861 cagtaaacgt cgctgtggtt tgctttaaat tctgaccatc cgcctttgca aaaatttgcc
     4921 tgattttaca aacgaatcag gctcatccca tcgacataaa aaaaatgccg atttatgcat
     4981 attctctcag ttcaacaatt ggattattaa taaatattgt ctagagtgag cggtcataaa
     5041 taagcacttt cttgccgctg aaaacgacca gcgcgggacc attcacaaca ccagaaggac
     5101 tcactttcag gtatggatcg tagacgattt attaaaggtt taatggctat ggccgccgtg
     5161 tgcggtacca gcggcattgc ttctcttttt tctcaggcag cattcgcggc agattctgat
     5221 attgccgacg ggcaaaccca gcgttttgac ttctccattc tacagtcaat ggcgcacgac
     5281 ttagcgcaaa cagcgtggcg tggtgcgcct cgtccgttac ctgacacgct ggcgacaatg
     5341 acgccgcagg cttataacag tattcaatac gacgccgaaa aatcgctctg gcataacgtt
     5401 gagaaccgtc aactggacgc tcagttcttc catatgggaa tgggattccg tcgccgcgtt
     5461 cgtatgtttt ctgtagatcc agcaacacat ctggcgcgtg aaattcactt tcgcccggag
     5521 ttgttcaaat acaacgatgc aggtgttgat accaaacaat tagaagggca aagcgatctc
     5581 ggctttgccg gttttcgcgt gtttaaagcc cccgaactgg cgcgccgtga tgtagtatca
     5641 tttctcggcg cgagttattt ccgcgccgtt gatgatacat atcaatacgg tttgtcggcc
     5701 cgcggcctgg cgatcgacac ttacaccgac agtaaagaag agttccccga ctttaccgcc
     5761 ttctggtttg atacggtaaa accgggggca actaccttta ccgtttatgc gttgctcgat
     5821 agcgccagca ttactggtgc ctataagttc actatccatt gtgagaaaag tcaggtgatt
     5881 atggatgtgg aaaatcacct gtatgcgcgc aaagacatta aacagctggg catttcgccg
     5941 atgaccagta tgttcagctg cggtactaat gaacgtcgga tgtgcgatac aattcatccg
     6001 caaattcatg actctgatcg tctgtccatg tggcggggca acggcgagtg gatttgccgt
     6061 ccgctgaata atccgcaaaa attgcagttc aatgcttaca ccgacaacaa cccgaaaggg
     6121 tttggtttat tgcaactgga tcgtgacttc tcccattatc aggacattat gggctggtat
     6181 aacaaacgcc caagtctgtg ggtggaaccg cgtaacaagt ggggtaaggg caccatcggc
     6241 ctgatggaaa tcccaacaac gggcgaaacg ctggataaca ttgtctgctt ctggcagcca
     6301 gaaaaagctg taaaagcagg tgatgagttt gcattccagt atcgtctgta ctggagtgcg
     6361 caaccgcctg ttcattgccc attagcgcgc gttatggcga cgcgtaccgg catgggcggt
     6421 ttcccggaag gttgggcgcc aggtgaacac tatcccgaaa aatgggcgcg tcgttttgcc
     6481 gtcgatttcg ttggtggtga tctgaaagct gccgcgccaa aaggcattga gccggtgatt
     6541 acgctttcca gtggggaagc gaagcaaatc gaaattctct atattgaacc catcgatggt
     6601 tatcgtattc agtttgactg gtatccgact tcggactcca ctgatccggt cgatatgcgg
     6661 atgtatctac gttgtcaggg ggacgctatc agtgaaacat ggctgtatca gtatttcccg
     6721 ccagcgccgg ataaacgtca gtatgttgac gaccgcgtga tgagttaatc gttttttctt
     6781 cggcaccttc ttcgggaggt gccgtctggt taaacacgat cccgctcgca tttttcccta
     6841 agttaaatga gtaatctgat ggtgtgtatt tcagatacac cgtgtcagcc actaacaggg
     6901 agtgcgtatg tttccagaat accgagattt aatatcccgt ctgaaaaacg aaaatcctcg
     6961 ctttatgtcc ttgttcgata aacacaataa acttgatcat gaaattgcca gaaaggaagg
     7021 ttccgacggt cgagggtaca atgcggaagt ggtccgcatg aaaaaacaaa agctacagtt
     7081 aaaagatgag atgctcaaaa tcctgcagca ggagagcgtc aaagaggtgt aaactttcct
     7141 aagccgccag ccaggcggct tttttaacaa cagcatggat tgactggaga taagatgact
     7201 gaaacgataa aagtaagcga atcacttgaa ttacatgctg ttgcggaaag tcacgtcaca
     7261 cctctttatc agttaatctg taaaaataaa acctggttac agcagtcgct aaactggccg
     7321 cagttcgttc agagtgaaga ggacacgcga aaaacggtgc agggtaatgt gatgttgcat
     7381 caacgcggct atgccaaaat gttcatgatt ttcaaagaag atgaacttat cggcgttatc
     7441 tcgtttaatc gtattgaacc actgaataaa actgctgaaa tcggctactg gctggacgaa
     7501 tctcatcagg ggcaggggat catttctcag gcgctgcagg cattgattca tcattacgct
     7561 cagtctggtg aacttagacg cttcgtgatc aaatgtcggg tggacaatcc gcaaagcaac
     7621 caggtcgctt tgcgcaatgg ttttatcctt gaaggttgcc tgaaacaggc tgagttcctg
     7681 aatgatgcct atgatgatgt gaacttatac gcgcgtatta tcgattcata ataaccctgc
     7741 cagcggcgtt cgcgtaatgc gctcctcgcc gttaatgact tttggaccac ggacatgaac
     7801 cgtgtcgcca tcaaaggctt caacgacggc gtggtcggtt aactccacat ggttttcaat
     7861 tatcaccgca cctgtaatac ggctctcacc ttgtattaca acgtgctcgt cgagcagaat
     7921 cggtccacca cgtaccacgg catttccgcc aatcaagaca tgatgtttta acacacaatt
     7981 accttccaca atggcatatt ccgccacctg cgaactgtaa tgaatcgtcg gaatggcatc
     8041 ttcttctatg ccagctttca cctgcgcgtg accgtagact ttagcgcaat cgcatagcca
     8101 gacgttgttc tcttcattac cttcaacact ggcaaaatca aaaacttcgg cgcgatgttc
     8161 aataaaagca taccggacga cggcatcgcc ataaatttgt gcctggtgga caatacgcga
     8221 ggcgctaacc cttgcgcgat cataaatttg gagcaggagt tgatggtcgg gcgttaagcc
     8281 ttgtgcggcg acgatcatag aatgttgatc aattaaggcg tgaccaaata ttctacattg
     8341 cacgtaaact aatgagtcat gaatggtgac actgtcactt atatatgcac cctggctgat
     8401 ttcactatta tcgatccaga cgttatccgt tgcgtaaacc tctccccaca gcacgctggt
     8461 gccggtgata cgcgtattac cggaaatcac cgttccgccg aacgcaatag cattctggtc
     8521 gtaaatccag caatttcctt cctgtgctaa caccgtttcg cgatcgatcc agccgccagc
     8581 ggttcctgct atcacatcgt taaaatcgct aatggcgata atctgccgta ataacacact
     8641 ttttttagtg ccatcttcct gataactaaa ggctcgctgt tcttcactta ggcgatattt
     8701 gcgcatagcg ttttcccaca ggtgacttac tataaccgta gcaaattctg cggctctggc
     8761 tatgctcgag aaaattccat aaaatgcatt tcaaatatac tttataaatt aaacaaaatg
     8821 agtaagaaga tgcagagcga taaagtgctc aatttgccgg caggctactt tggtattgtg
     8881 ttggggacga tagggatggg atttgcctgg cgctatgcca gccaggtttg gcaggtcagc
     8941 cactggttag gggatgggct ggtgattctg gcgatgatca tctggggatt attgactagc
     9001 gcatttatta cccgactcat acgctttccg catagcgtgc tggcggaagt tcgccatcca
     9061 gtgctgagca gttttgtgag tttgtttccg gcaacgacga tgctggtggc gattggtttt
     9121 gttccgtggt ttcgcccact ggcggtgtgc atgttcagtt ttggtgtcgt ggttcagttg
     9181 gcttatgccg cctggcaaac tgcgggatta tggcgcggat ctcaccctga agaagctacc
     9241 acgcctggac tgtatctgcc gacagttgcc aacaacttta tcagcgcaat ggcctgtggt
     9301 gcgttgggct acaccgacgc cggtctggtg tttttaggcg caggcgtttt ctcatggcta
     9361 agccttgaac cggtgatctt gcagcgtctg cgcagttcgg gagaattacc cacggcactg
     9421 cggacatcac tcggcattca gctcgctcct gcgctggtgg cctgtagtgc ctggctgagc
     9481 gtcaacggcg gcgagggtga cacgctggcg aaaatgcttt tcggttatgg actgctgcaa
     9541 ctgctgttta tgctacgtct gatgccatgg tatctctccc agccatttaa tgcttcattc
     9601 tggagtttct cgttcggcgt atctgcactg gcaaccaccg gtttgcatct ggggagtggc
     9661 agcgataatg gatttttcca tacgctggcg gtgccgctgt ttatctttac caattttatt
     9721 attgcaatac tgctcatccg tacttttgcg cttctgatgc agggaaaatt gttagtcaga
     9781 accgagcgcg ccgttttaat gaaagcagag gacaaagaat gatcattcgt gacgaaaact
     9841 attttactga taaatatgaa ttaacccgca cacactctga agtactggaa gcggtgaaag
     9901 tggttaaacc gggtaaaacg ctggatctgg gctgtggcaa tggtcgtaac agtctttacc
     9961 tggcagccaa tggttatgat gttgacgcat gggataaaaa tgccatgagt atcgccaacg
    10021 tcgagcgcat taaatccatt gaaaatctgg ataatttaca cacccgggtc gttgatctga
    10081 ataacctcac atttgatgga cagtacgatt ttattctttc gactgtggtg ctgatgttcc
    10141 ttgaggctaa aaccatccca gggttgattg ccaatatgca acgttgcact aaacctggtg
    10201 gttacaacct gattgtggcg gcgatggata ccgctgatta tccatgtacc gtcggcttca
    10261 cgtttgcctt caaagaggga gaattacgtc gatattacga aggctgggag agggtgaaat
    10321 acaatgaaga cgtcggcgag ctgcaccgca ccgacgccaa cggtaatcgt attaaactgc
    10381 gtttcgccac gatgctggca cgtaaaaaat gacccggtaa gcacaaaacg cgtgaaaatt
    10441 ccccacgctg agatgattta ctgttcttct tttcggtaag catatttttt atcgaaggga
    10501 tgtgaaatta atcacagtag tcgaagtttt ttagcagctt aacttactga aatttaacta
    10561 ctgatgattg acttagcccc tttttcggca ttgactatgt cgtctgaaaa ggggctgaaa
    10621 aatttatttt caccaacact ttttttgcca caacacgaag cggcgctttt tgctataact
    10681 tagaaagtaa tataatcatc tcaggaaact attcatgcgt accacatcat ttgcgaaagt
    10741 tgcagcttta tgcggcttgt tggctctgtc tggttgtgcc tctaaaatca cccagccaga
    10801 taaatattct ggttttttaa acaattactc tgatttaaaa gaaacaacct cagcaacagg
    10861 taaacctgtt ttacgttggg tagacccgag ttttgatcaa agcaaatatg acagcatcgt
    10921 ctggaaccca atcacttatt atccggtacc aaaaccttct acccaggtag ggcagaaagt
    10981 tctggataaa attttgaact ataccaacac cgaaatgaaa gaagcgatag cgcagcgtaa
    11041 accagtggtt accaccgctg ggccgcgtag tctgattttc cgtggggcca ttaccggtgt
    11101 agataccagc aaagaagggc tgcaattcta tgaagtggtt cctgttgcat tagtggttgc
    11161 ggggacgcaa atggctacag gccaccgtac catggatact cgcctctatt ttgaaggtga
    11221 gctgattgat gcagcgacta ataaaccggt tatcaaagtc gttcgtcagg gcgaaggtaa
    11281 agacctgaat aacgaaagta cgccaatggc tttcgaaaat attaaacaag ttattgatga
    11341 catggcgacc gatgccacca tgtttgacgt taacaaaaag tagttccaga cgcgccatcg
    11401 ttagatggcg ctttttatcc ggtgcgccgt aaaaccccat ccttcagggc ggggatataa
    11461 ggcgcggttt tccacctgac caggtgtttg ctgttgttca atgtattggc ggatgacgga
    11521 tattggcgca cctccgcagc tactggcaaa atagccagga ctccacaaaa cgcctttgta
    11581 gtaatacctg actgcaatat ctggtcgatc tcgtcgcagt aacctacccg atacgccttt
    11641 gaggctgttt accagactgg atatcgccag tttgggaggt tagttaatta acaaatggac
    11701 gtgatctggt tcgccatcca tttcaaccag ttcagcttca aaataagcac atacatttga
    11761 aaagtaagtg cgtagttttt ctgtcgcgtc atggtcaaaa atctggcgtc ggtatctggt
    11821 gacaaagact aggtgaacat gcatcaggaa aacacaatgc cttccacgcc ggatatcggt
    11881 ttcttttttc acagaccaaa gtatcatttg acctgtgaaa cgattacagg catttaaatt
    11941 ccagttaaga ccaggtggtc aacaggagtg tgaaatgagg cgcttcgcag gcgcatgtcg
    12001 tttcgttttc aatcgtgctc tggcacgtca gaatgagaat catgaggccg gtaataaata
    12061 catcccttac gggaaaatgg cttcctggct ggttgagtgg aaaaatgcca ctgaaacgca
    12121 atggcttaaa gattctccct cacagccatt gcaacagtca ctgaaagacc ttgagcgggc
    12181 ttacaaaaac ttcttccgga agcgggctgc ttttccccga ttcaaaaagc ggtgacagaa
    12241 tgatgcattc cgctacccgc agggcgttaa gctcgatcag gaaaacagcc gtatttttct
    12301 gccgaaactg ggctggatgc gctaccggaa tagccgtcag gtcacgggtg ttgtgaaaaa
    12361 tgtcactgtc agccagtcct gcggtacgtg gtacatcagt attcagacag aaagtgaagt
    12421 atccactccg gctcaccctt cagcatcaat ggtcgggctg gatgctggcg tggctaaact
    12481 cgccacgctg tcagatggca cagtctttga gcctgtaaac agttttcaga aaaaccagaa
    12541 gacgctggcg agacttcagc gacagttaag ccgcaaggtc aaattcagca acaactggca
    12601 gaagcagaaa cgcaaaatac agcgactgca ttcctgtatc gcaaatatcc gcagggacta
    12661 ccttcacaaa gtcacaacgg ccgtcagcaa aaaccacgca atgatagtca ttgaggattt
    12721 gaaggtcagc aacatgtcaa agtcagcagc gggtacggtc agccagccgg ggcgcaatgt
    12781 ccgggcaaaa tcaggtttaa a
//
LOCUS       DAEEUE010000106        12416 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.106, whole genome shotgun sequence.
ACCESSION   DAEEUE010000106 DAEEUE010000000
VERSION     DAEEUE010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12416
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.106"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..1199
                     /gene="edd"
                     /locus_tag="KLK84_003304"
     CDS             <1..1199
                     /gene="edd"
                     /locus_tag="KLK84_003304"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HBE2010517.1"
                     /translation="ALLESEAASYHAPGTCTFYGTANTNQMVVEFMGMQLPGSSFVHP
                     DSPLRDALTAAAARQVTRMTGNGNEWMPIGKMIDEKVVVNGIVALLATGGSTNHTMHL
                     VAMARAAGIQINWDDFSDLSDVVPLMARLYPNGPADINHFQAAGGVPVLVRELLKAGL
                     LHEDVNTVAGFGLSRYTLEPWLNNGELDWREGAEKSLDSNVIASFEQPFSHHGGTKVL
                     SGNLGRAVMKTSAVPVENQVIEAPAVVFESQHDVMPAFEAGLLDRDCVVVVRHQGPKA
                     NGMPELHKLMPPLGVLLDRCFKIALVTDGRLSGASGKVPSAIHVTPEAYDGGLLAKVR
                     DGDIIRVNGQTGELTLLVDEAELAAREPHIPDLSASRVGTGRELFSALREKLSGAEQG
                     ATCITF"
     gene            1236..1877
                     /gene="kdgA"
                     /locus_tag="KLK84_003305"
                     /gene_synonym="eda"
     CDS             1236..1877
                     /gene="kdgA"
                     /locus_tag="KLK84_003305"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBE2010518.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(1933..3111)
                     /gene="purT"
                     /locus_tag="KLK84_003306"
     CDS             complement(1933..3111)
                     /gene="purT"
                     /locus_tag="KLK84_003306"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HBE2010519.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            3245..3535
                     /gene="yebG"
                     /locus_tag="KLK84_003307"
                     /pseudo
     CDS             3245..3535
                     /gene="yebG"
                     /locus_tag="KLK84_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            3602..3958
                     /locus_tag="KLK84_003308"
     CDS             3602..3958
                     /locus_tag="KLK84_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HBE2010520.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            4285..4944
                     /locus_tag="KLK84_003309"
     CDS             4285..4944
                     /locus_tag="KLK84_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HBE2010521.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            5154..7214
                     /gene="ptrB"
                     /locus_tag="KLK84_003310"
     CDS             5154..7214
                     /gene="ptrB"
                     /locus_tag="KLK84_003310"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HBE2010522.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7211..7873)
                     /gene="exoX"
                     /locus_tag="KLK84_003311"
     CDS             complement(7211..7873)
                     /gene="exoX"
                     /locus_tag="KLK84_003311"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HBE2010523.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLNSMSPELRLTLKHYLE
                     NT"
     gene            complement(7897..8553)
                     /locus_tag="KLK84_003312"
                     /pseudo
     CDS             complement(7897..8553)
                     /locus_tag="KLK84_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            complement(8655..8885)
                     /gene="holE"
                     /locus_tag="KLK84_003313"
     CDS             complement(8655..8885)
                     /gene="holE"
                     /locus_tag="KLK84_003313"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HBE2010524.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            9024..9398
                     /gene="yobA"
                     /locus_tag="KLK84_003314"
     CDS             9024..9398
                     /gene="yobA"
                     /locus_tag="KLK84_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HBE2010525.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            9402..10274
                     /gene="copD"
                     /locus_tag="KLK84_003315"
                     /pseudo
     CDS             9402..10274
                     /gene="copD"
                     /locus_tag="KLK84_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            10287..10628
                     /locus_tag="KLK84_003316"
     CDS             10287..10628
                     /locus_tag="KLK84_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HBE2010526.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(11021..12097)
                     /locus_tag="KLK84_003317"
                     /pseudo
     CDS             complement(11021..12097)
                     /locus_tag="KLK84_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            complement(12063..12344)
                     /locus_tag="KLK84_003318"
     CDS             complement(12063..12344)
                     /locus_tag="KLK84_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBE2010527.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
BASE COUNT         3080 a         3032 c         3137 g         3167 t
ORIGIN      
        1 tggccttact ggagtcagaa gccgcgtctt accatgcgcc gggaacatgt actttctacg
       61 gtactgccaa caccaaccag atggtggtgg aatttatggg gatgcagttg ccaggctctt
      121 cttttgttca tccggattct ccgctgcgcg atgctttgac cgctgccgct gcgcgtcagg
      181 ttacacgcat gaccggtaat ggtaatgaat ggatgccgat cggtaagatg atcgatgaga
      241 aagtggtggt gaacggtatc gttgcactgc tggcgaccgg tggttccact aaccacacca
      301 tgcacctggt ggcgatggcg cgcgcggccg gtattcagat taactgggat gacttctctg
      361 acctttctga tgttgtaccg ctgatggcac gtctctaccc gaacggtccg gccgatatta
      421 accacttcca ggcggcaggt ggcgtaccgg ttctggtgcg tgaactgctc aaagcaggcc
      481 tgctgcatga agatgtcaat acggtggcag gctttggtct gtctcgttat acccttgaac
      541 catggctgaa taatggtgaa ctggactggc gggaaggggc ggaaaaatca ctcgacagca
      601 atgtgatcgc ttccttcgaa caacctttct ctcatcatgg tgggacaaaa gtgttaagcg
      661 gtaacctggg ccgtgcggtt atgaaaacct ctgccgtgcc ggttgagaac caggtgattg
      721 aagcgccagc ggttgttttt gaaagccagc atgacgttat gccggccttt gaagcgggtt
      781 tgctggaccg cgattgtgtc gttgttgtcc gtcatcaggg gccaaaagcg aacggaatgc
      841 cagaattaca taaactcatg ccgccacttg gtgtattatt ggaccggtgt ttcaaaattg
      901 cgttagttac cgatggacga ctctccggcg cttcaggtaa agtgccgtca gctatccacg
      961 taacaccaga agcctacgat ggcgggctgc tggcaaaagt gcgcgacggg gacatcattc
     1021 gtgtgaatgg acagacaggc gaactgacac tgctggtaga cgaagcggaa ctggctgctc
     1081 gcgaaccgca cattcctgac ctgagcgcgt cacgcgtggg aacaggacgt gaattattca
     1141 gcgccttgcg tgaaaaactg tccggtgccg aacagggcgc aacctgtatc actttttaag
     1201 acgacaaatt tgtaatcagg cgagagaaaa ctctgatgaa aaactggaaa acaagtgcag
     1261 aatcaatcct gaccaccggc ccggttgtac cggttatcgt ggtaaaaaaa ctggaacacg
     1321 cggtgccgat ggcaaaagcg ttggttgctg gtggggtgcg cgttctggaa gtgactctgc
     1381 gtaccgagtg tgcagttgac gctatccgtg ctatcgccaa agaagtgcct gaagcgattg
     1441 tgggtgccgg tacggtgctg aatccacagc agctggcaga agtcactgaa gcgggtgcac
     1501 agttcgcaat tagcccgggt ctgaccgagc cgctgctgaa agctgctacc gaagggacta
     1561 ttcctctgat tccggggatc agcactgttt ccgaactgat gctgggtatg gactacggtt
     1621 tgaaagagtt caaattcttc ccggctgaag ctaacggcgg cgtgaaagcc ctgcaggcga
     1681 tcgcgggtcc gttctcccag gtccgtttct gcccgacggg tggtatttct ccggctaact
     1741 accgtgacta cctggcactg aaaagcgtgc tgtgcatcgg tggttcctgg ctggttccgg
     1801 cagatgcgct ggaagcgggc gattacgacc gcattactaa gctggcgcgt gaagctgtag
     1861 aaggcgctaa gctgtaaaag tcaaaatgcc cgatctagga tcgggcattt ttgtagcgtt
     1921 tttttgccgg gtttaaccct gtacttttac ctgtccggcg gcgtgcttcg cgcgttcaat
     1981 ggcgtcaaca acactctctg aagtagccag taccaccccc agacgacggc tgccatcaat
     2041 ttccggctta ccaaataaac gaatctgcaa atctgcgcct acggcattct gcacattatc
     2101 aaacgtgaca ttctgactgg tcagttgtgg cagaataacg gcagaagctg caggaccata
     2161 ctgacggatc ccgccaacag gaagtccgag gaaggcacgt acatgcaggg caaactctga
     2221 gagatcttga gaaattaacg tcaccatccc ggtatcatgt ggacgagggg agacctcact
     2281 gaaaatcacc tcatcaccac agacaaacag ctcgacacca aacagaccat aaccgcccag
     2341 tgccagcacc actttacggg caatctcctg cgcacgttca agggcaagcg ggctcatttg
     2401 ctgtggttgc caggattcac ggtagtcgcc atcttcctgg cgatgaccta ctggtgcaca
     2461 gaaatggacg ccatccaccg cgctgacggt tagcagggta atttcgaagt caaacttaac
     2521 gacgccttca acaattacgc ggcccgctcc ggcgcgaccg ccttgctgag cgtattccca
     2581 tgcctgagca agttgctctg cagagcgaat aaacgtctgc cccttgccgg aagagctcat
     2641 caccggtttt acaatgcagg gatagccaat ggcggcaacc gcctcgcgga aaaggctttt
     2701 gctatcggca aaacgataag tggaagtggg cagctgcagc tcttctgccg ccaggcgacg
     2761 gataccctcg cgattcatcg ttaatttcgt tgcgcgagcg caggggacaa cattcagtcc
     2821 ttcctcttca agttggatca gcatatcggt ggcaatagct tcgatctccg gcacgatata
     2881 atgtggtttt tccagttcaa ccacacggcg taatgcatca ccatcaagca tattaatgac
     2941 atgggagcga tgcgcgacat gcatggctgg tgcgtcggca tagcgatcga cggcaatcac
     3001 ctctacgccg agacgctgac actcgattgc cacttcttta cccagttcac cggagcctaa
     3061 taacatcacg cgagttgctg ccggacgcag cgcagtgcct aataacgtca taacgaatat
     3121 cccctgatta attccgctcg cagtataaac gaaaacgttt gcgtgtgtct ttattgctga
     3181 tgttgatttc tcaaccgaaa agaaatatac tgtataaaat ctcagttatt atgagaggct
     3241 gcaaatggcg gttgaagttt aatacgtagt cattcgtgag ggcgaagaga aaatgtcgtt
     3301 taccagcaaa aaggaagccg atgcttatga caaaatgctt gatacggcgg atctgcttga
     3361 cacctagctg acaaattctc cagtgcaaat ggaagacgag caacgtgaag ccctttcgct
     3421 atggctggca gaacaaaaag aggtgctgag caccattctg aaaaccggca aattaccgtc
     3481 cccacaggtc gtcggtgctg aatccgaaga ggaagatgcc tcacacgcgg cctgaagcaa
     3541 cttgcgctcc tgacaatttg taccatgctt tctcctgaat cattgtgcta tggagaaaaa
     3601 catgaaaaaa agaggggcgt ttttagggcg gttgttggtt tctgcctgcg catcagtttt
     3661 cgctgccaat aatgaaacca gcaagtcggt cactttccca aagtgtgaag gtctggatgc
     3721 tgccggaatt gccgcgagcg taaaacgtga ttatcaacaa aatcgcgtgg cgcgctgggc
     3781 agatgatcaa aaaattgtcg gtcaggccga tcccgtggcc tgggtcagtt tgcaggacat
     3841 tcagggtaaa gatgataaat ggtcagtacc gctaaccgtg cgtggtaaaa gtgccgatat
     3901 tcattaccag gtcagcgtgg actgcaaagc gggaatggcg gaatatcagc ggcgttaatg
     3961 gcttctgata attcgattga ccgttggcgg atgcggcgcg agtgccttgt ccgtcccaga
     4021 acgcaccaca atcgtaggcc tgagaagacg caacagcgtc gcatcgggca atcagcatca
     4081 aacaatgcca ctcaaaacga caaactactt tcacctaaaa tacccttatc acttgatata
     4141 cagtaaatac cttacctggc gcgaatttct ttgaataacg gcctaccgcc ccgcttattt
     4201 gcgcatcttt tgtctcgata tacattttct gacatcctca tccgctatgc tacaggcact
     4261 aaaaatggtt attgaggagc aaatatggct aactggttaa atcaactgca atccctgctt
     4321 gggcaaagca gttcttctac ctcctcgtct gcggatcagg gattgggcaa actgttagtg
     4381 tcaggcgcat taggcggact ggctgggctg ctggtcgcaa ataaatcagc acgtaaactt
     4441 cttacgaaat atggcaccaa cgcgttactg gttggcggcg gagcggtagc gggtacggtg
     4501 ctgtggaata aatacaaaga taaaattcgc gcggcgcatc aggacgaacc gcagtttggc
     4561 gcgcaaagta cgccgctgga tgagcgtacg gaacgtttga tccttgcgct ggtctttgcc
     4621 gctaaaagtg atggtcatat tgatgccaat gaacgtgcgg caatcgacca gcaattgcgt
     4681 gaagccggcg tggaagagca ggggcgtgta ctcattgagc aggcaatcga acaaccgctg
     4741 gatccacaac gcctggctac cggggtccgc aatgaagaag aggcgctgga aatctatttc
     4801 ctgagctgcg cggctattga tattgaccat tttatggaac gcagttacct gaatgcactg
     4861 ggtgacgcgc tgaaaatccc ccaggatgtt cgtgaaggca ttgaacgcga tctcgaacag
     4921 caaaaacgca ctttggcgga ataattccgc tctctggata gcctgaagtt gactatttcg
     4981 ggctatccag aaccgcacag ccatttgaac aggctgattt tctctgtctg cataacattc
     5041 gatatccgcg ccattacctg tcatcatcta agcaatgacg accctgtttc gcttgcatcc
     5101 ccggtgagtt ttgccaccct tataagatgt ttccaaccag aaagaacaat aacatgctac
     5161 caaaagccgc ccgcattccc cacgccatga cgcttcatgg cgatacgcgc atcgataatt
     5221 actactggct gcgggacgat acgcgttctc agccaaaagt cctggactac ctgcaacaag
     5281 aaaatagtta cggtcatcgg gtgatggcct cacaacaagc cttgcaggat cgcatcttaa
     5341 aggaaatcat cgaccgcatt ccgcaaagag aagtttctgc gccctacata aaaaatggct
     5401 accgctatcg gcatatttat gaaccaggct gtgaatatgc tatctaccag cgtcaatcgg
     5461 cattcagcga agagtgggat gagtgggaaa cattgctcga tgccaacaag cgcgcagctc
     5521 atagtgagtt ttattcgatg ggcggaatgg cgattacgcc cgataacacc attatggcgc
     5581 tggcagaaga ttttctttcc cgacgccagt acggcattcg ttttcgtaat ctggaaacag
     5641 gtaactggta cccggaactg ctggataacg ttgaacccag ctttgtctgg gcaaatgact
     5701 cctggacttt ctactatgtt cgcaagcatc cggtgacgct gctgccttat caggtctggc
     5761 gtcacgccat cggtacgcca gcatcgcaag atacactgat ctacgaagaa aaagacgata
     5821 cctattatgt cagcctgcat aaaacgacgt cgaagcacta tgtcgtcatt catctggcca
     5881 gcgccaccac cagtgaagtt cgcctgctgg acgcggaaat ggccgatgcc gagccgtttg
     5941 tttttctgcc gcgccgcaaa gatcacgaat acagccttga tcactaccag catcgttttt
     6001 atctgcgttc caatcgccac ggcaaaaact ttggcttata ccgtacccgt atgcgtgatg
     6061 agcaacagtg ggaagagtta attccgccac gcgaaaacat catgctggaa gggtttacgc
     6121 tgtttaccga ctggctggtg gttgaagagc gtcagcgcgg gttaaccagt ttgcgccaaa
     6181 ttaaccgcaa gacccgggaa gtcattggca ttgcctttga tgatccggcc tatgtgacct
     6241 ggattgccta caatccagaa cctgaaaccg cgcgattgcg ttatggttat tcttccatga
     6301 ccacaccaga cactttgttt gaactggata tggataccgg tgagcgtcgt gtattaaaac
     6361 aaacagaagt gcctggtttt gatgcggcga attaccgcag tgaacacctg tggatagtcg
     6421 cccgtgatgg cgtcgaagtt ccggtttcgc tggtctatca tcgcaaacat tttcgcaaag
     6481 gacacaaccc gttgctggtg tatggctatg gttcttacgg cgcaagtatt gatgccgatt
     6541 tcagttttag ccgcttgagt ttgttagatc gtggctttgt ctacgccatt gtccatgttc
     6601 gcggcggtgg tgagctgggg caacaatggt acgaagacgg taaatttctg aagaagaaaa
     6661 atacgtttaa tgattatctt gatgcctgcg atgcattgtt aaaactgggc tatggctctc
     6721 cttcgctctg ttatgcgatg ggcggaagtg cggggggcat gttgatgggc gttgcaatta
     6781 atcaacgccc ggaattattc cacggcgtta tcgcccaggt accgtttgtt gatgttgtaa
     6841 caacgatgct tgatgaatca attcctctta ccactggtga gtttgaagag tggggaaatc
     6901 cgcaggatcc gcaatattac gagtacatga aaagctacag cccgtatgac aacgtcaccg
     6961 cacaggctta tccgcattta ctggtaacga ccggtttaca cgattctcag gtgcaatatt
     7021 gggaaccggc aaaatgggtc gctaaattgc gcgagctgaa aaccgatgac catcttttat
     7081 tgctctgtac cgacatggac tcaggccatg gcggtaaatc tggccgcttt aaatcgtacg
     7141 aaggcgtagc gatggaatat gcttttctgg tcgcgctggc gcagggaaca ttacccgcta
     7201 cgcctgcgga ttaagtattt tccagataat gtttcagtgt taaacgcagc tccgggctca
     7261 tgctgttcag gttattaaat aaccagcgca gatagcccgg atagcgttcg gcaacgtcgg
     7321 aaaccgcttt gccacggtat ttgccaaagg tgaaggtcgt catcaacgac ggacgtccgg
     7381 tgatatcggc catctgttct gccgtccagc cggaggtgtt cataatatcg ataagcaacg
     7441 cggcggtgat ataacagtca tacaacgcgc ggtgatgatg caggcccggc ggtgtctgta
     7501 cattgagctt gcgtgtttta tataacgcca tattgctgta cttgagccct ggccacaaac
     7561 gacgggccag tttcatagtg caaatccact caccgggcat ctcaggcagt acgcggcggt
     7621 caaagctggc gttgtgcgcg acataccatt cactaccgta atagtgtggg atcacatctt
     7681 caatccacgg tttatcggcg accatggctt cggtgatgcg atgaatcgcc atcgcttgtg
     7741 gactaatagg acgatcgggg cgcaccagga ggctcatggg gttgacgatt tttccgtcaa
     7801 tgacatcaac agaggcaatc tcaacgatcc ctccctgcaa accgcaggtt tctgtatcga
     7861 taatgcgcaa catggcatgc tccaggccga aaaagcctag cgtaatggaa tgatatcgcc
     7921 ttgccaaccc tgtgatgaac gctgaccgac taacaataat gaaccacgat cggcgcgaac
     7981 gatgagacga ccatattcat cccatagtgc actgttacca cgggcatttg ccatcagcac
     8041 ggcaatcgag tactgatgtg aaaaaaattg taagcgacgg gccgacgcca gcaggtcggg
     8101 ttctcccaga cattgccctg tcgtaaacag tgaacaggtc gggtccatat ccataccttg
     8161 cggttgttca tcgaccaccg tgatggttct ggaacgacgg cccagacatg caccatgact
     8221 ctgatggtag ttcccaggcg tttttcgcca cggtgcgaac accgcaattc cacgaataaa
     8281 gcgatcgtta tattcaacgg gaaggccggc aatgatggtc attcgctagg tcgttgcggc
     8341 ataacaaagc gggtctaaca gtgaaagatc gggtggggca ggaagggcac gtcgcgaata
     8401 atcacacccc agtaaagaaa gcgatggaaa aactaacagc tgacattgtt gcctggcggc
     8461 ggccctcaca aactccagat gatgagctac ctgttcggtg agtgaggttt tacgaggctc
     8521 atattgcgct gctgcaactt tccagaacga cataatgact tccttttcaa aatgactgca
     8581 aattatgggg gcggtttaga aaatactcat aatttagcag gcgttatgta agaaagtgta
     8641 actctgtata agttttattt aagtttgggc tcgtaaggta aacgtgacag attgaccgaa
     8701 gccaaacggt gggcaataag ccgctcgcga aaccagctgc gcaaatgttc tggctgttca
     8761 cgttcaaccg cttcagcgat caccggcata ttgtagcgtt ctttaaaagc caccccggcc
     8821 gccgccaaat cgacattcac tttatccatt tctgtttgat ccagtttagc cagattcttc
     8881 agcattctta atctcctcat cattcgcggg caaaagttac taagagtgag cctggatggc
     8941 aagccggaat tgatgatcct cgactcagac attagacaag gttatgctct gtaaaaaagt
     9001 tattacaaaa aggaatagat aatatggctt caactgcacg ctcccttcgc tacgcgctgg
     9061 caatcctgac cacttcgttg gtgacacctt ccgtctgggc acatgcgcat ttaacgcatc
     9121 agtatcctgc ggcaaacgcg caagtgacag ctgccccgca ggcaatcact ttaaacttct
     9181 cggaaggcgt tgaaacagga ttcagtggtg caaaaatcac ggggccaaaa aacgaaaata
     9241 ttaaaacatt accagcgaag cggaatgagc aggaccaaaa gcaactgatt gttccactgg
     9301 ccgattcgct gaaacccgga acctacaccg ttgactggca tgttgtttcg gtggacgggc
     9361 ataaaacgaa aggacactac acctttagcg tgaaataaat catgctggcg tttacctgga
     9421 tcgcgctgcg atttatccat ttcacctcgt tgatgctggt ctttggcttt gcaatgtatg
     9481 gtgcctggct ggccccctta atgattcgtc gcttgctgac gaagcgttct ctgcgtttac
     9541 aacagcacgc tgccgtctgg agtttgatca gtgccactgc aatgctcgcc gttcagggcg
     9601 ggttgatggg aacgggatgg acagatgtat tttcgccaaa catttggcag gctgttttgc
     9661 aaacgcagtt tggtggcgtc tggttatggc aaattgttct cgccctcgta acgttgatcg
     9721 tcgcccttat gcaaccgcgg aatatgccac gtttgctgtt tatgctcact accgcgcaat
     9781 ttattctgct ggcgggagtg gggcatgcga cgctgaatga aggggtaaca gcgaaaatcc
     9841 atcagactaa tcacgcgatt cacctgattt gtgcggcggc gtggtttggg ggattactgc
     9901 cggtgctctg gtgtatgcaa ctcatcaaag gccgttggcg acactaggct attcaggcgc
     9961 tgatgcgttt ttcctggtgc gggcattttg cggtgatcgg cgtactggca agcggcgtgc
    10021 ttaatgcatt gttgattacc ggatttcccc ccacgcttac gacttactgg ggccaactct
    10081 tattgctcaa agccatcctg gtgatgatta tggtggtcat tgcgctggct aatcgttatg
    10141 ttcttgtacc gcgcatgagg caggatgaag atcgtgcggc accctggttt gtgtggatga
    10201 ctaaactcga atgggcgata ggtgcggtag tgctggtgat catcagcctg cttgcgactc
    10261 tcgaaccttt ttgatggatt acgaaaatga tgaaaaaaag tattctggcg tttctgttac
    10321 tcaccagttc tgcagcggcg ctggcggctc cacaggtgat aaccgtgagt cgttttgaag
    10381 tgggtaaaga caaatgggcg tttaatcgcg aagaggtaat gctgacttgc cgaccgggta
    10441 atgctttgta tgtcatcaac ccaagtaccc tcgtgcagta tcctttaaac gatatcgcac
    10501 aaaaggaagt tgccagtggg aagaccaatg cccaacccat ttcggtgatt cagattgatg
    10561 atcctaacaa tcccggcgaa aaaatgagtc tggcaccgtt tatagaacga gctgaaaaac
    10621 tctgttaatt acctaaaata gccttttgat ttccaataaa aaaaccgcct cagttctttc
    10681 accagaacgg gcggttttta acatttaagc tgatgagcac cgcgcttttt attgaccatt
    10741 ttgcacgcaa actggaaaac ctggcgtcgt catctattct taaagggcaa ggcaactaag
    10801 cctgcattaa tgccaacttt tagcgcacgg ctctctccca agagccattt ccctggaccg
    10861 aatacaggaa tcgtattcgg tcttttttta attgtattta aaatcaatta gttgcaaacg
    10921 tctccccgaa attccccgaa atttactcga atttctgtat tccggtcttt tttggttata
    10981 tcacaaccaa aatacattta acaatccatt tacgttaaaa tcagagcagt aagtacgttt
    11041 tttctctctc atcaagatac atttttgttg tcttctccga tgtgtggcca agtagacgct
    11101 gagcaaattc ttctccacat atttctttgt acaatcgtcc agccaggctt ctgatttcgt
    11161 gaaaagttgg tgggttttca ctgaacagga tacctgttaa ttttctggct gcgacaaatt
    11221 tctttgtcag gccgtccggg tgaatgctgc cgtcagggct gttttttcta atcccggcac
    11281 tgattagata atccccccta cttaccatgc ggcactgttc aactactgca ccaagtcgta
    11341 aaccagcgac aggaaggctg agtgacaggg ggatagcaat catcattcct gtcttaattt
    11401 gcctgatgtg gagacgatca tcataaatat cactgaaccg catattcgtt atgtcttcgc
    11461 gacgttgtcc tgttacaagg gctaagtcca tagctaatgg gaaccatgcc ggaagttgat
    11521 ctgctgcctc cctgatgcag ttgtatgtct ttagtttcag ccgttctctt gtaactacta
    11581 ttttcggtgc tcttgttggc gttactggat tttgagatat acgtccttca acaatggctt
    11641 cgcggaacat atcagataac acagaacgca ttgatcctgc catcgtgttt ttccctcctt
    11701 caatctacaa atcaagaaac tcggcgatat ggcgagtggt tatttctgtc agtaaaaccc
    11761 ctcctaattt ttcttttatt gtctccagtt gatttacccg agttttataa gtatttctgg
    11821 acgcttttct ccttataaga atcgttttgt aacgttcaat ccagcctgcc atagtaaatg
    11881 agtcgacccc tttaagcttt tcaattaggg cagcaggaga gtagtttttg tatatataat
    11941 gatttgcttc aattgcctgc gctactgcat ctcttcttga aattttacct agtgtaaatt
    12001 cttctttcgt cagagggttg cgccagtaat atgctttgtc tctccttcga tatgttaagt
    12061 ttctaggcaa attgggatcg tattttttcc gctgcatgtt ttaacttctc cattaacgga
    12121 ctgtctctcc cttgtcgccc attaggctga tggtgtgtta tatcggtatc aaccttattt
    12181 gggttgatat agaaagcctc cggaaccacc ctgtaactcc tcccgtgtag ttcaggtgca
    12241 ggataaatgt ttccattcct tgcccatcgt ctcagcgttg atattgatgg tgggttatcc
    12301 ggatatctga gttttcccca cgttttgagt gtcacaagat tcattgccat acctctcacg
    12361 atatgaccgc cagtaaatat acagaatact ggcgggtgtg gttgattttt aataat
//
LOCUS       DAEEUE010000107        12220 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.107, whole genome shotgun sequence.
ACCESSION   DAEEUE010000107 DAEEUE010000000
VERSION     DAEEUE010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12220
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.107"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..493)
                     /locus_tag="KLK84_003319"
     CDS             complement(<1..493)
                     /locus_tag="KLK84_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE2010528.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(524..1216)
                     /gene="fimC"
                     /locus_tag="KLK84_003320"
     CDS             complement(524..1216)
                     /gene="fimC"
                     /locus_tag="KLK84_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HBE2010529.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1436..1978)
                     /gene="fimA"
                     /locus_tag="KLK84_003321"
     CDS             complement(1436..1978)
                     /gene="fimA"
                     /locus_tag="KLK84_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HBE2010530.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2459..3325
                     /gene="folD"
                     /locus_tag="KLK84_003322"
     CDS             2459..3325
                     /gene="folD"
                     /locus_tag="KLK84_003322"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HBE2010531.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3327..3539
                     /gene="ybcJ"
                     /locus_tag="KLK84_003323"
     CDS             3327..3539
                     /gene="ybcJ"
                     /locus_tag="KLK84_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HBE2010532.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3647..4156
                     /locus_tag="KLK84_003324"
     CDS             3647..4156
                     /locus_tag="KLK84_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBE2010533.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4192..5577)
                     /gene="cysS"
                     /locus_tag="KLK84_003325"
     CDS             complement(4192..5577)
                     /gene="cysS"
                     /locus_tag="KLK84_003325"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBE2010534.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5751..6245
                     /gene="ppiB"
                     /locus_tag="KLK84_003326"
     CDS             5751..6245
                     /gene="ppiB"
                     /locus_tag="KLK84_003326"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HBE2010535.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6248..6970
                     /gene="lpxH"
                     /locus_tag="KLK84_003327"
     CDS             6248..6970
                     /gene="lpxH"
                     /locus_tag="KLK84_003327"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HBE2010536.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7088..7597
                     /gene="purE"
                     /locus_tag="KLK84_003328"
     CDS             7088..7597
                     /gene="purE"
                     /locus_tag="KLK84_003328"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBE2010537.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7594..8661
                     /gene="purK"
                     /locus_tag="KLK84_003329"
     CDS             7594..8661
                     /gene="purK"
                     /locus_tag="KLK84_003329"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBE2010538.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYACGVIWAQSKFS"
     gene            complement(8798..9691)
                     /locus_tag="KLK84_003330"
     CDS             complement(8798..9691)
                     /locus_tag="KLK84_003330"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBE2010539.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9688..10335)
                     /locus_tag="KLK84_003331"
                     /pseudo
     CDS             complement(9688..10335)
                     /locus_tag="KLK84_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10338..11570
                     /gene="allC"
                     /locus_tag="KLK84_003332"
     CDS             10338..11570
                     /gene="allC"
                     /locus_tag="KLK84_003332"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HBE2010540.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQK"
     gene            11581..>12220
                     /gene="allE"
                     /locus_tag="KLK84_003333"
     CDS             11581..>12220
                     /gene="allE"
                     /locus_tag="KLK84_003333"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HBE2010541.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2969 a         3156 c         3117 g         2978 t
ORIGIN      
        1 gagcgctact caacaacgct atctgaggaa tagtgatgtt caggcgcatc gctgcaaaat
       61 caaagttaat tgtggcatca ggtacagctt tcagtaaatt gatgcattta ttgttttgct
      121 gctgggctaa ctcgggaaat gcactgctat taaggccaat tcgttcaagg agtacctggt
      181 taaaacaggg cattaaccca cctgatttat cacctgtatt ttctgttgtc gattcaaata
      241 cgatatcctg tgaaccaatg aactcatcat tacgccagag atccacccga tacacgcccg
      301 caggttgatg attacctctc tcaaagcgcg ataaatcagc aacggatgtg ccattctcta
      361 ataaaaatgc cgggttgaaa taactttccg catagcttaa aaaggagggt aaaatggcaa
      421 gagatgaata acaaattccg gccagacacc aggtatacct ctgcggaata tccgtagtag
      481 tgggtatttt cataattagc tctgatattg tcagatgatt cggttagttt aagttcactt
      541 ctctgaccgg ggttactgaa ccataatcat taacggtctg aaatttcagc tttccctgca
      601 ctcctgaggg taagggaatt tgtgaactaa ctctgggtgc cgccatagca ttccccaact
      661 tttggctgcc cacttgcagg ttaacgaggg tgacataaaa aggtgatgga ttatgaacat
      721 tgatatagtt accggatcgc gaaaacttga gtgtgtccgg cgcttctgcg ggtaattctt
      781 gtaattgaat tggacggaga aataatttca tgcgcgataa aatcgccagt tgcaaaacat
      841 tcctgccgtt caatgcattt ttatctaccg aagggatcgt cttaacattc atccagaaca
      901 gagactcacg atctgccgcc agcggtggac cggtgtaaat aatacgcaaa gtattttcgc
      961 ttttgggttc actaacaaat agtggcggtg taatgatgaa tgacttttct tttacaccgc
     1021 tgctgttttc aatccacgaa ttgaccagaa agagctcatt ggtatggcta tttttaatcc
     1081 ataccgcagt ctgtttagca tcagcgggat aaataatacg cgtggcacct aatgcgatcc
     1141 ctccggcagc atgggcgctg gccgaaatga ttaaataaaa tatcatgagc atcaataact
     1201 ttattttagt catcatagtt aatacgcctt gtgtattcct gacggcaaaa ataaggagta
     1261 taaagaaatg attgctttgg cttcttacaa cacatcattc ccttttcatt taataaagcg
     1321 gcatgataca aagtgaacgt ggtttcgatt tacattgcca ccataccgct tcagacaacc
     1381 ggtacattac tttgttataa gacgtgatat ataattcaaa acaacgtggt tttgattatt
     1441 cgtatctcat aataaaagtc gcgtcagcat ttgcttgccc tgctgatgca cttgtaccgg
     1501 tgcctttata acgtgcagaa aaatgaagaa cgttggtccc ggggatcaag ttctggttgg
     1561 ttgagaagct attcccatca ggtttgagaa ttgcggatgt attatcaagt atttcaatac
     1621 cgacacccgt tgctgttgtt gtatttgtac tggatgcaat ggccaataaa ttatcattga
     1681 ttgcatcagc ctgaccagaa aatgcaacgg cagcattagc ggcaacaaca ggatcgcagt
     1741 cgttcaactg aatggtgaat ggaattaatg cagaggtatt accaacagca ttgaaaatat
     1801 cggtacgata ttgaccgagt gtgacaacct ggtctgccga gtcagtattc actgaacagg
     1861 cagcattcac cagttcgcct tcaaagtgaa ttgtaccgcc atctacaacg gcagcataac
     1921 tacccgtagc ggcgaatagt gcggcagcca gcgcagacga aataaatctt aatttcatat
     1981 atattccttc aatctcattt atcgactcca catccgtata taaccgatta ctttatttaa
     2041 gacactgata gtagtaaaat tcctttttat cctctaagaa tgtcttaatt gaaaatatgc
     2101 actctattct aaaaaataga gagccccgtt agatgaatac ttccgcgcaa aatatattca
     2161 acacaaatat agacctgaag cggtaaatta ccaggctgaa aattcttttt atattgtcag
     2221 gtatttctta aattatctta atccttaaac aaggaaataa atcagttcca gatttacaac
     2281 gccatcatgt acgaaaaatg aagctttcaa tctcagcgac ctcagcgacg gtgcgcctaa
     2341 ccttcgcaag aggtcgcttc acgcgataaa tctgaaacga aacctgacag cgcgccccgc
     2401 ttctgacaaa atagacgcat ccccttcgac ctacgtaaca gatggaatcc tctctctgat
     2461 ggcagcaaag attattgacg gtaaaacgat tgcgcagcag gtgcgctctg aagttgctca
     2521 aaaagttcag gctcgtattg cagccggact gcgggcacca ggactggccg ttgtgctggt
     2581 gggtagtaac cctgcatcgc aaatttatgt cgcaagcaaa cgcaaggctt gtgaagaagt
     2641 cgggttcgtc tcccgctctt atgacctccc ggaaaccacc agcgaagcgg agctgctgga
     2701 gcttatcgat gcgctgaatg ccgacaacac catcgatggc attctggttc aactgccgtt
     2761 accggcgggt attgataacg tcaaagtgct ggaacgtatt catccggaca aagacgtgga
     2821 cggtttccat ccttacaacg tcggtcgtct gtgccagcgc gcgccgcgtc tgcgtccctg
     2881 caccccgcgc ggtatcgtca cgctgcttga gcgttacaac attgatacct tcggcctcaa
     2941 cgccgtggtg attggcgcat cgaatatcgt cggtcgccca atgagcatgg aactgttgct
     3001 cgcaggttgc accaccaccg tgactcaccg cttcactaaa aatctgcgtc atcacgtaga
     3061 aaatgccgat ctgttgatcg ttgccgttgg caagccaggc tttattcccg gtgactggat
     3121 caaagaaggc gcaattgtga ttgatgtcgg catcaaccgt ctggaaaatg gcaaagttgt
     3181 gggcgacgtc gtgtttgaag acgcggctaa acgcgcctca tacattacgc ctgttcccgg
     3241 cggcgttggc ccgatgacgg ttgccacgct gattgaaaac acgctacagg cgtgcgttga
     3301 atatcatgat ccacagggtg agtaacatgg cgacattttc tttaggtaaa catccgcacg
     3361 ttgagctgtg cgacttgctg aaactggaag gctggagcga aagcggcgcg caggcgaaaa
     3421 tcgcgattgc cgaaggccag gtgaaagtcg acggtgcggt tgaaacgcgc aaacgctgca
     3481 aaatcgtcgc cggtcagaca gtgagttttg caggacacag cgtacaggtt gttgcctgat
     3541 ccctttcccg gcctgtatca acaggccggt tctcttttca gacttttgcc ctctacgccc
     3601 cgttcatcca tgctgatagc atcgacattt tgccgaagcg accatcatgc caaccgttat
     3661 tacgcacgcc gccgttcccc tttgcattgg tttagggttg ggttcgaaag gtctgttatt
     3721 tgccggaatc atcctggcga tgctgcccga tgccgacgta ttgtcgttta aatttggcgt
     3781 tgcttacggc aatgtttttg gtcatcgcgg ttttacccat tcgctggtgt ttgcgtttgt
     3841 tgtcccgctc ttatgtgtgc tgattggacg tcgatggttc agggctgggc tgattcgctg
     3901 ctggctgttt ttaaccgtct cgttgctatc gcacagctta ctggattcgg taaccactgg
     3961 cggtaagggc gttggctggc tgtggccgtg gtcagatgaa cgctttttcg ctccctggca
     4021 ggtgattaaa gtcgcgccgt ttgcattgtc tcgttacacc acagcgtacg ggcatcaggt
     4081 gattatttcc gaattgatgt gggtgtggtt gccggggatg ctactgatgg gaatgttgtg
     4141 gtggcgcaga cgataaccgg atgcgaaaac tcgcatccgg caattgcgca attacttacg
     4201 acgccaggtg gtcccttgcg ggccatcttc cagcacgatc cccatctcgt taagacggtc
     4261 acgtgccgca tctgccgccg cccagtcttt cgctttacgg gcatccagac gctgttgaat
     4321 taacgcttca atctcagcca cttcgctgtc gtctgcctgc gcgccgcttt gcaggaacgc
     4381 ttccggttct tgctccagca ggcccaatac agcggaaagt ttacgcaggt gagatgccat
     4441 tgcattcgcc gctgccatat cttctgcttt cagacggttc acttcacgcg ccatatcaaa
     4501 cagcacggaa taggcttccg gggtgttgaa atcgtcgtcc atcgcttcaa taaagcgcgc
     4561 ttcaaacgct tcgccaccgg caggcgcaac ggttttatct gtgccgcgca gcgcagtgta
     4621 gaggcgctcc agcgccgcac gcgcctgctt caggttctct tcgctgtagt tcagctggct
     4681 gcgatagtgg cccgacatca ggaagtaacg cacggtttcc gcgtcgtagt atttcagcac
     4741 atcgcgcacg gtaaagaagt tacccagcga tttggacatc ttctcgcggt caaccatcac
     4801 cattccggag tgcatccagt agttcacata ctgaccatca tgggcacagg tggactgcgc
     4861 gatttcgttc tcgtggtgcg ggaacatcag gtctgaaccg ccgccgtgga tatcaaagtg
     4921 gttacccagc tgcttgcagt tcatggccga acattcaatg tgccagccag gacgacccgc
     4981 gccccacgga gacggccagc tcggttcgcc ctctttcgac atcttccaca gaacgaagtc
     5041 catcgggttg cgtttgtcat caaccacgtc aacgcgcgcg ccagcctgca gctggtcgag
     5101 atcctgacgc gacagcacgc cataagttgg atcggtcggg acgtcgaaca tcacgtcgcc
     5161 gttgtccgcc acataagcgt gacctttggc gatcagttgt tcagtgagtt caataatttc
     5221 tgcgatatgg tgcgtcgcgc gcggctccat atccgggcgc agaatgttca aagcatcaaa
     5281 atctttgtgc atttcggcga tcatgcgatc caccagcgcc acaaagcttt cgccattttc
     5341 attggcgcgt ttgatgattt tgtcgtcgat atcggtaatg ttgcgcacat acttcagttt
     5401 atagccgagg aaacgcagat agcgcgcaac cacgtcaaaa gcaacaaagg tacgcccgtg
     5461 accgatatga cagagatcgt aaacggtgat tccacacacg tacatgccga cttccccggc
     5521 gtgaataggc ttaaattcct ctttttggcg tgtcagagta ttgaagattt ttagcatcga
     5581 agattccgtt tagacatgtg tgggtaattg agttgcgtat aatacccata tttcccgcgc
     5641 gaatcagcat acattgcgtg atgatccgat cgtgcggtta tgctataaca ccaccctata
     5701 tatgacccga actgggttga agcaccaatc aaacggaaca ggatgcaaaa atggttactt
     5761 tccacaccaa tcacggcgat attgtcatca aaacttttga cgataaagca cctgaaacag
     5821 ttaaaaactt cctggactac tgccgcgaag gtttttacaa caacaccatt ttccaccgtg
     5881 ttatcaacgg ctttatgatt cagggcggcg gttttgaacc gggcatgaaa caaaaagcca
     5941 ccaaagaacc gatcaaaaac gaagccaaca acggcctgaa aaatacccgt ggtacgctgg
     6001 caatggcacg tactcaggct ccgcactctg caactgcaca gttcttcatc aacgtggttg
     6061 ataacgactt cctgaacttc tctggcgaaa gcctgcaagg ttggggctac tgcgtgtttg
     6121 ctgaagtggt tgacggcatg gacgtggtag acaaaatcaa aggtgttgca accggtcgta
     6181 acggtatgca ccaggacgtg ccaaaagaag acgttatcat tgaaagcgtg accgttagcg
     6241 agtaatcgtg gcgacactct ttattgcaga tcttcatctc tgcgtggaag aaccggcgat
     6301 caccgccggt tttctgcgtt ttttagcggg ggaagcccgc aaggccgacg cgctgtatat
     6361 tcttggcgat ctgtttgaag catggattgg cgacgacgat cccaacccac tccatcgcca
     6421 gatggcggca gcgatcaaag cggtgtccga ttccggcgtt ccctgttatt tcattcatgg
     6481 caaccgtgat tttctgctcg gcaaacgctt tgcccgtgaa agcggcatga cattattgcc
     6541 ggaagaaaag gtgctcgaac tttatggtcg ccgggtgtta attatgcatg gcgacacgct
     6601 gtgcaccgat gacgcgggtt atcaggcttt tcgcgccaaa gtccacaaac cctggctgca
     6661 aatgctattc ctcgccctgc cgttgtttgt gcgcaaacgc attgccgcgc gaatgcgcgc
     6721 gaacagcaaa gaagccaaca gcagtaaatc gctggcgatc atggacgtta accaaaacgc
     6781 tgtggtcagt gcgatggaaa aacatcaggt gcaatggcta atccacgggc atacccatcg
     6841 cccggcggtg catgaactta tcgccaacca gcaacctgct tttcgcgtgg tactgggtgc
     6901 ctggcatacg gaaggttcga tggtgaaagt cacggctgat gacgttgagc tgattcattt
     6961 tccgttttaa aaaacccaca actttgctga tttcacagcc acgcaaccgt tttccttgct
     7021 ctctttccgt gctattctct gtgccctcta aagccgagag ttgtgcacca caggagtttt
     7081 aagacgcatg tcttcccgca ataatccggc gcgtgtcgcc atcgtgatgg ggtccaaaag
     7141 cgactgggct accatgcagt tcgccgtcga aatcttcgaa atcctgaatg tcccgcacca
     7201 cgttgaagtg gtttctgctc accgcacccc cgataaactg ttcagcttcg ccgaaagcgc
     7261 cgaagagaac ggttatcagg tgattattgc gggcgcaggc ggcgcagcgc acctgccagg
     7321 catgattgcc gccaaaacgc tggtgccggt gctgggcgtg ccagtacaga gcgccgcact
     7381 gagcggtgtc gatagcctct actccatcgt acaaatgccg cgcggcattc cggtgggtac
     7441 gctggcgatt ggtaaagctg gcgcggcaaa cgcggcatta ctggcagcac aaattctcgc
     7501 gactcacgat aaagagctac accagcgtct gaatggctgg cgcaaagccc agaccgacga
     7561 agtgctggaa aacccggacc cgcgaggtgc ggcatgaaac aggtttgcgt cctcggtaac
     7621 gggcagttag gccgtatgct gcgtcaggca ggcgaaccgt taggcattgc tgtctggccg
     7681 gtcgggctgg acgctgaacc ggcggcggtg ccttttcaac aaagcgtgat taccgctgag
     7741 atcgaacgct ggccggaaac cgcattaacc cgcgagctgg cgcgtcatcc ggcctttgtg
     7801 aaccgcgatg tgttcccgat tattgctgac cgtctgactc agaaacagct tttcgataag
     7861 ctccacctgc cgaccgcgcc gtggcagtta cttgccgatc gcagcgagtg gcctgcggtg
     7921 tttgatcgtt taggtgaact ggcgattgtt aagcgtcgca ctggtggcta tgacggtcgc
     7981 ggtcaatgga gtttacgcgc caatgaaacc gaacagttac cggcagagtg ttacggtgaa
     8041 tgtattgtcg agcagggcat taacttctct ggtgaagtat cgctggttgg cgcgcgcggc
     8101 tttgatggca gcaccgtgtt ttatccgctg acgcataacc tgcatcagga cggtattttg
     8161 cgcaccagcg tcgcttttcc gcaggccaac gcacagcagc aggcgcaagc cgaagagatg
     8221 ctgtcggcga ttatgcagga gctgggctat gtgggcgtga tggcgatgga gtgttttgtc
     8281 accccgcaag gtctgctgat caacgaactg gctccgcgtg tgcataacag cggtcactgg
     8341 acacaaaacg gtgccagcat cagccagttt gagctgcatc tgcgggcgat taccgatctg
     8401 ccgttaccgc aaccagtagt gaataatccg tcggtgatga tcaatctgat tggtagcgat
     8461 gtgaattatg actggctgaa attgccgctg gtgcatctgc actggtacga caaagaagtc
     8521 cgtccggggc gtaaagtggg gcatctgaat ttgaccgaca gcgacacatc gcgtctgact
     8581 gcgacgctgg aagccttaat cccgctgctg ccgccagagt atgcctgcgg cgtgatttgg
     8641 gcgcagagta agttcagtta agcaggtgct catttgattg ccggatgcgg cgtaaacgcc
     8701 ttatccggct taccgctccg gtacccattg taggcctgat aagacgcgtc aagcgtcgca
     8761 tcaggcatgg tgcaccgatt gccggatgct ctcatcacta cagcgaaata caggtccccg
     8821 cttcgcccgc cagcgtctct tcaattcgcg ataacgcccc aatccacgcg ggtttactac
     8881 ggcttctgac ataaccactc accgccgtta ccttcggccc catcgaacca tcggctttgg
     8941 caaatggagc taactcatcc ggtgtggcat ggcgaatggc acgttgctgc ggcgttcccc
     9001 agttttcata taccgcatca gcatcggtga ggatcaccag tccatcggca ttaatctgct
     9061 cggcgagcaa cgcagcggcg agatctttat caatcaccgc ttcactccct gccccatctt
     9121 ccgtcacagg cacaccgccg ccgccactgc aaatcaccac atgcccctct ttgagcaaca
     9181 actcgatggc ttcgctgtcg agaattttac gcggttgcgg agacgccacc acccggcgca
     9241 aatatttacc atcacgtttc atctgccagc cgtaagccgc ttccagtgcc tcttgttctt
     9301 ctggctgata aaccggacca ataaatttct ctggctgcaa aaacgccgga tcatcaggcg
     9361 aaacctcaat gcgcgtcagc accgtcgtaa caggcggcat ctgcggctgt gcgctcaagc
     9421 tctgcgccag catatagcca atcatcccct ggctttccgc caccagtaca tccagcggat
     9481 acggttctac ctctttccac gccagattct gtaacgccag cagccccacc tgcggcccgt
     9541 tgccgtgaac aatcgccagc cgataagaac gggccaggcg tgccagcgcg ggtacagcac
     9601 tggcgatatt gcgatattga ttttccgccg tcagcgcctc accgcgctgg agtaaggcgt
     9661 tgcccccaag agctacaacc agtgttttca tggttttcct tgtaataatt gttggccaag
     9721 ccagaacccc agcagcgtat ctgcccctga cgtatgcccc agcgccagca gcgaatcaat
     9781 cgcaacagcg gtacgtttcg ggcaactcag agcatggaca aagtgcagga gtggcgaggc
     9841 gaaatatcct tgcgcggcat aacgtaaata actgacgctc accgctgtgg taacgagttg
     9901 aagattgtcg gaacaggcaa aaaacgggcg accggcgcgc gcatctaaag cgccataata
     9961 ccaggccgcc agcagcattc cgctcaacgt gtcatcatga cttggcgtta atccggggcc
    10021 tttacccagc cagtaccgcc agtcggtctt aacgccattg agcgcggcct gaaaacagtg
    10081 acgaaactgg cgtaattcag cgggcagcgg atcgcttgcc gccatcgcca gtggcccgaa
    10141 aagtccggtt tcctccgcgc gttgcatcca tgcagctgca agcggttgag gatgcgaagg
    10201 cggcgtaata cgcagcaaac aataacgctg tggctgttta accgtgaaac gcccgaggcg
    10261 aatcccttga gccacaacct gtggtcgttc attgccgcat aatccaccgc ataacgcatc
    10321 gaattgcgtg cggcgaaatt acacattccc gtcaagctat agaagaaacg ctgccctggc
    10381 tttcctcttt tggcgctgac ccaacgggtg ggatgacccg tttactttat tcgccggaat
    10441 ggctggaaac ccagcagcaa tttaaaaaaa gaatggcagc aagtgggctg gaaacacgtt
    10501 tcgatgaagt ggggaattta tacggtcgcc tgagtggcac cgaatatcca caggaagtgg
    10561 ttctgagcgg ttcgcatatc gataccgtgg ttaacggcgg taacctcgac gggcaattcg
    10621 gcgcgctggc ggcgtggctg gcaattgact ggttgaaaac gcaatacggc gcaccgttac
    10681 gtacggtcga agtggtggcg atggcagaag aagaaggcag ccgcttcccg tatgtcttct
    10741 ggggcagtaa aaatatcttt gggctggcga atcctgacga cgtgcggaat atctgtgatg
    10801 ccaaaggaaa tagttttgcc gatgcgatga aggcttgcgg atttactctg ccgaacgccc
    10861 cactaactcc gcgtcaggct ataaaagcct ttgttgaact gcatattgaa cagggctgtg
    10921 tgctggaaag taatgggcaa tcaattggcg tggtgaatgc aattgtcggg cagcgtcgct
    10981 atacggtgac gctgaacggc gaatcaaacc atgcaggcac cacgccgatg ggttatcgtc
    11041 gtgatacggt ttacgctttc agtcgcattt gccatcagtc gatcgaaaaa gcgaaaaaga
    11101 tgggcgatcc gctggttctg acctttggca aagtagagcc gcgcccgaat acggtaaatg
    11161 tggtgccggg taaaaccacg ttcaccattg attgtcgtca taccgacgct gccgtgctgc
    11221 gcgatttcac ccaacagtta gaaaacgaca tgcgggcgat ttgcgatgaa atggacattg
    11281 gtattgatat cgatttatgg atggacgaag aacccgtgcc gatgaataag gagctggtcg
    11341 ccaccctgac agaattgtgt gaaagagaaa aactgaatta ccgggtgatg cacagtggtg
    11401 ccgggcacga cgcgcaaatt ttcgcgcctc gcgtaccaac ctgcatgatt tttatcccca
    11461 gcatcaacgg gatcagccat aacccggcgg aacgcaccaa tattactgac cttgccgaag
    11521 gggtcaaaac gttggcactc atgctttatc aacttgcctg gcagaaataa ggagtcataa
    11581 atgggatatt taaataacgt caccggttac cgcgaagatt tactggctaa ccgtgcgatt
    11641 gttaaacacg gtaatttcgc actgttaacc ccggatggtc tgataaaaaa tattattccg
    11701 gactttgaaa attgtgacgc gacaatcctc tccacgccaa agctgggtgc ctcttttgtt
    11761 gattatctgg tcacactgca tcaaaacggt ggcaaccaac agggcttcgg tggcgaaggc
    11821 attgaaacgt tcctgtatgt gatctctgga aatatcactg ccaaagccga aggcaaaaca
    11881 tttgccttaa gcgaaggtgg ctatctttat tgcccaccag gctccttaat gacgtttgtt
    11941 aacgcccagg ccgaagacag ccaaatcttt ttatataagc gccgctatgt tccggtagaa
    12001 ggccatgcac cgtggctgtt ttctggcaat gccagcgaac tggaacgcat tcactatgaa
    12061 ggtatggatg atgtgatcct gctggatttt ctgcccaaag agttaggttt tgatatgaac
    12121 atgcatatcc tctcttttgc accaggtgcc agccacggtt atatcgaaac acacgttcag
    12181 gaacatggtg cctatattct ttccggtcag ggggtttata
//
LOCUS       DAEEUE010000108        12167 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.108, whole genome shotgun sequence.
ACCESSION   DAEEUE010000108 DAEEUE010000000
VERSION     DAEEUE010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12167
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.108"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            215..1356
                     /locus_tag="KLK84_003334"
                     /pseudo
     CDS             215..1356
                     /locus_tag="KLK84_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            complement(1480..2250)
                     /gene="glcC"
                     /locus_tag="KLK84_003335"
     CDS             complement(1480..2250)
                     /gene="glcC"
                     /locus_tag="KLK84_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HBE2010542.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            2501..4000
                     /gene="glcD"
                     /locus_tag="KLK84_003336"
                     /pseudo
     CDS             2501..4000
                     /gene="glcD"
                     /locus_tag="KLK84_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            4000..5052
                     /gene="glcE"
                     /locus_tag="KLK84_003337"
     CDS             4000..5052
                     /gene="glcE"
                     /locus_tag="KLK84_003337"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HBE2010543.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            5063..6282
                     /gene="glcF"
                     /locus_tag="KLK84_003338"
                     /pseudo
     CDS             5063..6282
                     /gene="glcF"
                     /locus_tag="KLK84_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            6287..6691
                     /locus_tag="KLK84_003339"
     CDS             6287..6691
                     /locus_tag="KLK84_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HBE2010544.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            6713..8884
                     /gene="glcB"
                     /locus_tag="KLK84_003340"
     CDS             6713..8884
                     /gene="glcB"
                     /locus_tag="KLK84_003340"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HBE2010545.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            9239..10834
                     /locus_tag="KLK84_003341"
     CDS             9239..10834
                     /locus_tag="KLK84_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HBE2010546.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSLVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVIGVITLLQAYVFTGMLVS"
BASE COUNT         2960 a         2904 c         3285 g         3018 t
ORIGIN      
        1 atttaattta tgaatatatt aagaagaagc cttgtccggt cgccttaaat tgacaaaagg
       61 cattgaggcg tttctgtgta gcctctgacc ttatcataat cattaattaa tactatttat
      121 tcgtcgtttg tattcattga gttgattatt aagatgccgc tcgttgctgt tgcaacctgt
      181 ttttcaccat taattatttg ttatccacag agatatggaa tgcgatctgc tgatgattaa
      241 aattgaaaaa gtaattaata aaaatgacct taaggcattt atcgcttttc cgtcatcact
      301 ttatcccgac gatccaaact ggataccccc tttattcatt gaacgcaacg agcatttgtc
      361 tgcgaaaaat ccggggacgg accatatcat ctggcaggcg tgggtggcaa aaaaagcggg
      421 gcagatagtt gggcgtatta ccgcgcaaat agataccttg caccgtgagc gttacggtga
      481 agataccggt catttcggca tgattgacgc cattgatgat ccgcaggatt ttgccgcgtt
      541 gtttggcgca gcggaagcgt ggttgaagtc acaaggtgca agtaagatca gcgggccttt
      601 cagcctgaat atcaatcagg aaagcggatt actgattgaa ggttttgaca caccaccctg
      661 tgcgatgatg ccacacggca aaccgtggta tgccgcgcat attgaacaac tgggttatca
      721 caaaggcatt gatttactgg cgtggtggat gcagcgaacc gatctcactt tctctccggc
      781 gctaaaaaaa ctgatggatc aggtgcgcaa aaaggtgacc attcgctgca tcaatcgtca
      841 gcggtttgcc gaagagatgc agatcctgcg tgagattttc aactctggct ggcagcacaa
      901 ctggggattt gtgccgttta ccgaacatga attcgcgacc atgggcgacc aacttaaata
      961 tctggtgccg gacgatatga tctacatcgc tgagattgat tctgcaccct gcgcgtttat
     1021 tgtggcttgc ctaacatcaa cgaggcgatt gccgatctga acggatcgct ctttcccttc
     1081 ggctgggcaa aattgctgtg gcgtttgaaa gtcagcggtg tgcgaactgc gcgagtaccg
     1141 ctgatgggcg tgcgcgacga gtatcagttc agccgcatcg gcccggtgat tgcgctgtta
     1201 ttgattgaag ccttacgcga tccgtttgcc cgccggaaga tcgatgcgct ggagatgtca
     1261 tggatcctcg aaaccaatac aggcatgaat aacatgctgg aacgcattgg ggcggagccg
     1321 tacaagcgtt accgattgta tgaaaagcaa atttaatacc agatcctgtt tagcgcaggg
     1381 gcggtcattc gggagagggt atagatgaac agatttaaca ccagatcctg ttaagtgcag
     1441 gggcggtccc ctcgccttgg ggatagggta aaactctgct cacattccca ccttgttcat
     1501 ctccagcggc accgccgagc gaatcaaatg gtggccttcc agctcgatat cgtggagatt
     1561 ctttttcacg gtccgtacat gatcgcgcgc tgcgcgctga gcgacgtgcg gcagccgctg
     1621 caacaccgcg ttgtagatcc gcgcatgctg gcgatcgatc tgcttttttt gctgtggtcg
     1681 atggtagaga ttatttacgc tggcaaacac tgaattaaac atcagatcgg tcaatgattg
     1741 cagcgtaaac accagcacct gattgtgaga agcctgacaa atggcgagat ggaaagcgtg
     1801 atccaactgc gcatgttcga tcagcgaaat ctctttgtgg ttctcactgg cggcgagcat
     1861 tttttcataa cagcgggtta tcacaacaaa atcagcctgc gttcccagcg ttgccgccag
     1921 ccttgccgat tcgccctcca gtaatgcgcg aacgtcgagc agatcgtaca gcgttcgcgg
     1981 ctgcgtactg aacagatgga tcagcgggct ggtgtcctgc tcccgattaa gccgtgcgac
     2041 atgagaatcg cgaccctgcg ccgtttcaat aatcccgcgc ccgcgcagca cggtcagccc
     2101 ttcacgcagt gcggagcgtg agaagccgag cttttcacac agtcgacgtt ccgagggaag
     2161 cggctgaccg accttcagta cgccgtcgat aattaaccgt tcgatactct ctgcaaccac
     2221 ttcgcaaata gggcgacgtt catctttcat tcccggacct cgtgcacagg taggaccaat
     2281 ttttctgttc ttaactcgca aaacacgcac atcacgcaag tgattatgtt acatcaattt
     2341 aacattaagt taaccaagac aaggtcacag agctggaaaa aaaatggtct gaccggtagc
     2401 taaagagata gacgaaaacg aaaagcccgc ttaataactg ttcacagaag cagcgcgcaa
     2461 aaatcagctg ccacacaaca caacaaagcg aagcctactc atgagcatct tgtacgaaga
     2521 gcgtcttgat ggcgctttac ccgatgtcga ccgcacatcg gtactgatgg cactgcgtga
     2581 gcatgtccct ggacttgaga tcctgcatac cgatgaggag atcattcctt acgagtgtga
     2641 cgggttgagc gcgtatcgca cgcgtccatt actggttgtt ctgcctaagc aaatggaaca
     2701 ggtgacagcg attctggctg tctgccatcg cctgcgtgta ccggtggtga cccgtggtgc
     2761 aggcaccggg ctttctggtg gcgcgctgcc gctggaaaaa ggtgtgttgt tggtgatggc
     2821 gcgctttaaa gagagcctcg acattaaccc cgttggtcgc cgcgcgcgcg tgcagccggg
     2881 cgtatgtaac ctggcgatct cctaggccgt tgcgccgcat aatctctatt acgcgccgga
     2941 tccttcctca caaatcgcct gttcaattgg cggcaatgtg gcggaaaatg ccggtggcgt
     3001 ccactgcctg aaatatggtc tgaccgtaca taacctgctg aaaattgaag tgcaaacgct
     3061 ggacggcgag gcgctgacgc tgggatcgga cgcgctggat tcacctggtt ttgacctgct
     3121 ggcgctgttc accggatcgg aaggtatgct cggcgtgacc accgaagtga cggtaaaact
     3181 gctgccgaag ccgcccgtgg cgcgggttct gttagccagc tttgactcgg tagaaaaagc
     3241 cggacttgcg gttggtgaca tcatcgccaa tggcattatc cccggcgggc tggagatgat
     3301 ggataacctg tcgatccgcg cggcggaaga ttttattcat gccggttatc ccgtcgacgc
     3361 cgaagcgatt ttgttatgcg aactggacgg cgtggagtct gacgtacagg aagactgcga
     3421 gcgggttaac tacatcttgt tgaaagcggg cgcgactgac gtccgtttgg cacaggacga
     3481 agcagagcgc gtacgtttct gggccggtcg caaaaatgcg ttcccggcgg taggacgtat
     3541 ctccccggat tactactgca tggatggcac catcccgcgt cgcgccctgc ctggcgtact
     3601 ggaaggcatt gcccgtttat cgcagcaata tgatttacgt gttgccaacg tctttcatgc
     3661 cggagatggc aacatgcacc cgttaatcct tttcgatgcc aacgaacctg gtgaatttgc
     3721 ccgcgcggaa gagctgggcg ggaagatcct cgaactctgc gttgaagttg gcggcagcat
     3781 cagtggggaa catggcatcg ggcgagaaaa aatcaatcaa atgtgcgccc agttcaacag
     3841 cgatgaaatc acgaccttcc atgcggtcaa ggcggcgttt gaccccgatg gtttgctgaa
     3901 ccctgggaaa aacattccca cgctacaccg ctgtgctgaa tttggtgcca tgcatgtgca
     3961 tcacggtcat ttaccttttc ctgaactgga gcgcttttga tgctacgcga gtgtgattac
     4021 agtcaggcgc tgctggagca ggtgaatcag gcaattagcg ataaaacgcc gctggtgatt
     4081 cagggcagca atagcaaagc ctttttaggt cgcccagtca ccgggcaaac gctggatgtg
     4141 cgttgtcatc gcggcattgt taattacgac ccgaccgagc tggtgataac cgctcgtgcc
     4201 ggaacgccgc tggtggcgat tgaagcggcg ctggagagcg ccgggcaaat gcttccctgc
     4261 gagccgccgc attatggtga agaagccacc tggggcggga tggtcgcctg cgggctggcg
     4321 gggccgcgtc gcccgtggag cggttcggtc cgcgattttg tcctcggcac gcgcatcatt
     4381 accggcactg gaaaacatct gcgttttggt ggcgaagtga tgaaaaacgt tgccggatac
     4441 gatctctcac ggttaatggc cggaagctat ggttgtcttg gcgtgctcac tgaaatctca
     4501 atgaaagtgt taccgcgacc gcgtgcctct ctgagcctgc gtcgggaaat cagcctgcaa
     4561 gaagccatga gtgaaatcgc cgagtggcaa ctccagccat tacccattag tggcttatgt
     4621 tacttcgaca atgcgttgtg gatccgcctt gagggcggcg aaggatcggt aaaagcagcg
     4681 cgtgaactgc tgggtggcga agaggttgcg ggtcagttct ggcagcaatt gcgtgaacaa
     4741 caactgccgt tcttctcgtt accaggtacc ttatggcgca tttcattacc cagcgatgcg
     4801 ccgatgatgg atttacccgg cgagcaactg atcgactggg gcggggcgtt acgctggctg
     4861 aaatcgacag ccgatgacaa tcaaatccat cgcatcgccc gcaacgctgg cggtcatgcg
     4921 acccgcttta gtgccggaga tggtggcttt gccccgctat cggctccttt attccgctat
     4981 caccagcagc ttaaacagca gctcgaccct tgcggcgtgt ttaaccccgg tcgcatgtac
     5041 gcggaacttt gaggagcagg ctatgcaaac ccgattaacc gaagagatgc ggcagaacgc
     5101 gcgctggaag ccgacagcat cctgtgcgcc tgtgttcact gcggattttg taccgcaacc
     5161 tgcccaacct atcagcttct gggcgatgaa ctggacgggc cgcgcgggcg catctatctg
     5221 attaaacagg tgctggaagg caacgaagtc acgcttaaaa cacaggagca tctcgatcgc
     5281 tgcctcactt gccgcaattg tgaaaccacc tgtccttctg gtgtgcgcta tcacaatttg
     5341 ctggatatcg ggcgtgatat tgtcgagcag aaagtgaaac gcccactgcc ggagcgaatg
     5401 ctgcgcgaag gattgcgcca ggtagtgccg cgtccggcgg tcttccgtgc gctgacgcag
     5461 gtagggctgg tgctgcgacc gtttttaccg gaacaggtca gagcaaaact gcctgctgaa
     5521 acggtgaaag ctaaaccgcg tccgccgctg cgccataagc gtcgggtttt aatgctggaa
     5581 ggctgcgccc agcctacgct ttcgcccaac accaacgcgg caactgcgcg agtgctggat
     5641 cgtctgggga tcagcgtcat gtcagctaac gaagcaggct gttgtggcgc ggtggactat
     5701 catcttaatg cgcaggagaa agggttggca cgggcgcgca ataatattga tgcctggtgg
     5761 cccgcgattg aagcaggtgc cgaggcaatt ttgcaaaccg ccagcggttg cggcgcgttc
     5821 gtcaaagagt atgggcagat gctgaaaaac gatgcgttat atgccgataa agcgcgtcag
     5881 gtcagtgaac tggcggtcga tttagtcgaa cttctgcgcg aggaaccgct ggaaaaactg
     5941 gcaattcgcg gcgataaaaa actggccttc cactgcccgt gtaccctaca acatgcgcaa
     6001 aagctgaacg gcgaagtgga aaaagtgtta cttcgtcttg gatttacctt aacggacgtt
     6061 cccgacagcc atctgtgctg cggttcagcg ggaacatatg cgttaacgca tcccgatctg
     6121 gcacgccagc tgcgggataa caaaatgaat gcgctggaaa gcggcaaacc ggaaatgatc
     6181 gtcaccgcca acattggttg ccagacgcat ctggcgagcg ccggtcgtac ctctgtgcgt
     6241 cactggattg aaattgtaga acaagccctt gaaaaggaat aaaaaaatga aaactaaagt
     6301 cattcttagc cagcaaatgg cgagtgcaat tattgccgca ggtcaggaag aggcgcagaa
     6361 aaataactgg tctgtttcca ttgctgttgc cgatgacggc ggtcatctgc tggcgttaag
     6421 tcgcatggac gattgcgcgc cgattgcggc ttacatctcc caggagaaag cgcgtaccgc
     6481 cgcgctgggg cgtcgtgaaa ctaagggcta tgaagagatg gtgaacaacg gacgtaccgc
     6541 gttcgtgact gcgccgttac tgacgtcgct ggaaggcggc gtaccggttg ttgttgatgg
     6601 gcaaattatt ggtgccgtgg gcgtttctgg tttaaccgga gcacaggatg cgcaggtcgc
     6661 gaaagtggca gcagcggtgt tggcgaaata agcgaaaacg aggagagaaa caatgagtca
     6721 aaccataacc cagggccgtt tacgcattga cgccaatttt aaacgttttg tggatggaga
     6781 agttttgccg ggcgtggaac tggacgctgc tgcattctgg cacaatgttg atgagatcgt
     6841 tcacgatctg gcaccagaaa atcgtcagtt gctggcagaa cgcgatcgca ttcaggcggc
     6901 gcttgatgag tggcatcgca gcaatccggg gccggtaaaa gataaagcgg cctataaatc
     6961 tttccttcgt gaactgggct acctggtgcc gcaaccggag cgcgtgacgg tggaaaccac
     7021 gggcattgac agcgaaatca ccagtcaggc ggggccacag ctggtggttc cggcaatgaa
     7081 cgcccgctac gcgctgaacg cggcgaacgc tcgctggggc tcactgtacg atgcgttata
     7141 cggcagcgac atcatcccgc aggaaggggc gatggtcagt ggctacgatc cgcagcgcgg
     7201 tgagcaggtt atcgcctggg ttcgacgttt cctcgatgaa tcactaccgc tggaaaacgg
     7261 cagctatcag gatgtggtgg cgtttaaggt ggtcgataaa caattacgca tccagttgaa
     7321 aaatggtaaa gaaaccacgt tacgtactcc agcacagttt gtcggttacc gtggcgatgc
     7381 cgctgcgccg acctgcattt tgctgaaaaa taacggcctg catattgaac tgcaaattga
     7441 tgccaacggg cggattggca aagacgatcc ggcgcacatc aacgatgtta tcgtcgaagc
     7501 tgctatcagt accattctcg actgcgaaga ttcggtcgcg gcggttgatg cggaagataa
     7561 aatcctgctg taccgcaatc tgctgggcct gatgcagggg actctgcaag agaaaatgga
     7621 gaaaaacggt cggcaaatcg tacgtaaact gaatgacgat cgtcagtaca ccgccgccga
     7681 tggctctgaa atttctctgc acggacgctc gttgctgttt atccgcaacg tgggtcattt
     7741 gatgaccatt cctgtgattt gggacagcga aggcaatgaa atcccggaag gtattcttga
     7801 tggcgtcatg actggcgcga ttgccctcta tgatttaaaa gtgcagaaaa actcgcgcac
     7861 tggcagcgtc tatattgtga aaccgaaaat gcacggtccg caggaagtgg cgttcgccaa
     7921 caaactgttt acccgcattg agacaatgct cggtatggca ccgaataccc tgaaaatggg
     7981 cattatggat gaagaacgcc ggacctcgct gaacttgcgt agctgtatcg ctcaggcgcg
     8041 caaccgcgtg gcgttcatca ataccggttt cctcgaccgt accggcgatg aaatgcattc
     8101 ggtgatggaa gctggcccga tgctgcgtaa aaaccagatg aaatcgacgc cgtggatcaa
     8161 agcctacgaa cgtaataacg tgctttccgg tctgttctgt gggctgcgcg gtaaagcgca
     8221 aatcggtaaa ggcatgtggg caatgccgga cctgatggca gacatgtaca gccagaaggg
     8281 cgaccaactg cgtgccgggg caaacacagc ctgggttccg tcaccaaccg ctgctacgct
     8341 ccatgcgctg cactaccacc ataccaacgt acagagcgtg caagccaaca ttgcccagac
     8401 cgagttcagt gctgaatttg aaccgctgct ggacgatctg ctgactattc cggtggctga
     8461 aaacgctaac tggtcggcgc aagagatcca acaagagctg gataacaacg tgcaggggat
     8521 tctggggtac gtggtgcgtt gggtggagca ggggattggt tgttcaaaag tgccggatat
     8581 tcacaacgtg gcgctgatgg aagaccgcgc aacgctgcgt atctccagcc agcatatcgc
     8641 caactggtta cgtcacggta ttctgaccaa agaacaggtg caggcgtcgc tggagaatat
     8701 ggcgaaagtg gttgatcagc aaaatgctgg cgatccggct tatcgtccga tggcggggaa
     8761 tttcgctaac tcgtgtgctt ttaaagctgc cagcgattta atcttcctcg gcgtgaaaca
     8821 gccaaacggt tataccgaac cgttattaca cgcctggcgt ttacgcgaaa aagaaagtca
     8881 ttaatagcgt caaaaaagcc ccggcaagtt ttattgtcgg ggctatgata gtaactgcgg
     8941 tagtacattt atcccggaaa taaccggagc gattatcagg gtttaacaat attaaatacc
     9001 cgtagcgcat tactagcggg cctaaaacaa caatatggac atcttaacga tactctgcgc
     9061 ctgcgtgtgc ggaacagaaa cccttacccg atatacttgt tatgccagag gtagcaattg
     9121 cgttagcggc gtactgttgc tgtaaagtat aacgcctata ttttacagtt cagtcctctt
     9181 ttgcttatta caaccttaag tatatcaagc atataaagat aataagagac tgaacaatat
     9241 ggttacctgg acccaaatgt atatgccgat gggaggactg gggctatccg ctctggtcgc
     9301 cctgatcccg ataatattct tcttcgttgc actcgcggta ttacgtctga aaggacatgt
     9361 cgctggagca ataaccctta tattatctat cctgattgcg atattcgcct ttaaaatgcc
     9421 gattgatatg gcatttgctg ctgcgggcta tggctttatt tatggattat ggccaattgc
     9481 gtggattatt gtcgcggcgg tgttcctgta taaattaacc gttgccagcg ggcagttcga
     9541 tattatccgc agcttggtta tctccatcac cgacgatcag cgtttgcagg tgttactgat
     9601 tggtttctcc tttggtgcgt tgctggaagg agctgctggc tttggtgcga cggtggcgtt
     9661 tggtgcgttg ggcgtgccga ttctggtcgc cggtcaggta acgggaatcg atccgttcca
     9721 cattggcgca atggcgggac gtcagttacc gttcctgtcg gttcttgtgc cgttctggct
     9781 ggtagcaatg atggacggct ggaaaggggt gaaagagacg tggccagcgg cgctggttgc
     9841 tgggggaagc ttcgctgtca ctcagttctt tacctctaac tatattggtc cggaactgcc
     9901 ggatattact tcggcgctgg tgagtatcgt ctcactcgct ttatttctta aagtctggcg
     9961 gccgaaaaat accgaaacgg caatcagcat ggggcaatcc gcaggtgcga tggtggtaaa
    10021 taagccatct tctggcggtc ccgtgccttc agaatatagt ctggggcaaa tcattcgtgc
    10081 gtggtcaccg tttttaatct taacggtgct ggtcaccatc tggaccatga agccgtttaa
    10141 agcgttattt gctccgggcg gcgcgtttta ttcactggtg attaatttcc agatccctca
    10201 tttgcatcaa caagtgttga aagcggcacc cattgtcgcc caaccaacgc caatggatgc
    10261 ggtgtttaaa ttcgaccctc tctcggctgg cggcaccgct atttttattg cggcgattat
    10321 ctctatcttc atcctcggcg tgggcatcaa gaaagggatt ggcgtctttg ccgaaatgct
    10381 aattagtctg aagtggccga tattgtcgat tggcatggtg ctggcattcg ccttcgtcac
    10441 caactattcc ggcatgtcca ccacgctggc gctggtactg gcaggtacag gcgtgatgtt
    10501 cccgtttttc tcaccgtttc tcggctggct gggcgtattc cttaccgggt cggacacttc
    10561 ttcaaacgcc ctgtttggtt cactgcaatc gaccacggcg cagcaaatca acgtctctga
    10621 cactctgctg gtggcagcaa ataccagcgg cggcgtaact ggcaagatga tctccccgca
    10681 atctatcgcc gtggcctgcg ccgcgacggg catggtgggc cgagaatctg aactgttccg
    10741 ctacaccgtg aagcacagtc tgatttttgc cagcgttatc ggcgttatca ccctgctgca
    10801 ggcgtatgtg tttaccggga tgttagtctc gtaaatcaat aaagccggat gaaggtaaat
    10861 atcacccggc tttccatttg tctgtcgttt atctcgccag ggaaccgttt ttacgattgg
    10921 cttctttata cgtagtctcc gttttacgat gcgaattgtt taatatttat acaacttggc
    10981 ttctgtgctg gttataaagt acccactttc atgagatcaa ttttagtatg ttattacatt
    11041 gcttcatctg ttgctttcat gacgaatggc gttttttctc ttatttatat ttttcactag
    11101 aacaatgagt agatcaaaat tcccaaaact cccaaaaaca taagattatt cgcaaagctg
    11161 attttgcaga ataatgagaa cttttaagat ttgtcgagga tcaattttaa gcaatttatc
    11221 tccacttgtt acataaaaga tgccgttagg ttaataagtt aatcttgcta taacagtgtt
    11281 cgttactgcc tattaataaa gatatttggg atagggtaac gtggatgagg attcgaaatc
    11341 atccgtcttg cggaaagtga aatattggca gatgttaata ttatgtatgt attttaactc
    11401 caacaacaca ccaatcattt tgttagaaat aagaaaattt atggttaaat aatttggtga
    11461 ttatgaagca ttaaatcgta atgccaaata ttgttcgctt tggagagtat tattgcctgt
    11521 ttgtgtctgt tgagcagaaa aatcatctga atttgaatac ttttattagg tgtgaataaa
    11581 tgaaaaaatc attaaaatca ttaaaatcat taaaatcatt aaaatcatta acttatatga
    11641 atatttcaga gttggagttc gaatgttgac acctgagtat ttcctgctaa aaatagccct
    11701 gctttacgga tggttgtatt gcaataacac gaaagtagta agagattaaa ggtatctgtc
    11761 agcgataact ctgcaagtga tgacattttg taaaccaaat aatgtaaatc agcaatgtat
    11821 cttttcactt aattataaca aatgtggtat tcactaagaa tctgatacga gcttacatca
    11881 cgactatttt agtctcatcg cgggatacag ggatgcaccg atggatctca gcacaccgta
    11941 atgcaatacc tgaacaaata gaacagtaaa gtggttcagt attaatgaaa gctcttattt
    12001 tccgttggga aaacttaaaa tagatgagaa ggctttttga tttcttatgt gatctcattt
    12061 cgagtattga gaaatttgtg atttttaaat ttttcaataa gccctgtaag ggattggggt
    12121 tttatgagaa ataaagagac gtattgtctt tgtttaaacg gtacgta
//
LOCUS       DAEEUE010000109        12153 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.109, whole genome shotgun sequence.
ACCESSION   DAEEUE010000109 DAEEUE010000000
VERSION     DAEEUE010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12153
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.109"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            126..995
                     /locus_tag="KLK84_003342"
     CDS             126..995
                     /locus_tag="KLK84_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HBE2010547.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1198..1551)
                     /locus_tag="KLK84_003343"
     CDS             complement(1198..1551)
                     /locus_tag="KLK84_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HBE2010548.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1689..3335)
                     /gene="groL"
                     /locus_tag="KLK84_003344"
     CDS             complement(1689..3335)
                     /gene="groL"
                     /locus_tag="KLK84_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBE2010549.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3379..3672)
                     /locus_tag="KLK84_003345"
     CDS             complement(3379..3672)
                     /locus_tag="KLK84_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBE2010550.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3948..5204
                     /gene="yjeH"
                     /locus_tag="KLK84_003346"
     CDS             3948..5204
                     /gene="yjeH"
                     /locus_tag="KLK84_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HBE2010551.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5220..5696)
                     /gene="fxsA"
                     /locus_tag="KLK84_003347"
     CDS             complement(5220..5696)
                     /gene="fxsA"
                     /locus_tag="KLK84_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HBE2010552.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6033..7469
                     /gene="aspA"
                     /locus_tag="KLK84_003348"
     CDS             6033..7469
                     /gene="aspA"
                     /locus_tag="KLK84_003348"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HBE2010553.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7587..8888
                     /gene="dcuA"
                     /locus_tag="KLK84_003349"
     CDS             7587..8888
                     /gene="dcuA"
                     /locus_tag="KLK84_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HBE2010554.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9004..9342
                     /gene="cutA"
                     /locus_tag="KLK84_003350"
     CDS             9004..9342
                     /gene="cutA"
                     /locus_tag="KLK84_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HBE2010555.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9318..11015
                     /gene="dsbD"
                     /locus_tag="KLK84_003351"
     CDS             9318..11015
                     /gene="dsbD"
                     /locus_tag="KLK84_003351"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HBE2010556.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11052..11627
                     /locus_tag="KLK84_003352"
     CDS             11052..11627
                     /locus_tag="KLK84_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBE2010557.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11734..11809
                     /locus_tag="KLK84_003353"
     tRNA            11734..11809
                     /locus_tag="KLK84_003353"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11767..11769,aa:Phe,seq:gaa)
BASE COUNT         2932 a         3140 c         2980 g         3101 t
ORIGIN      
        1 ttctgtttat atagagtgag cacggctatt tatgttattt taacaactat tactaccaag
       61 cccatttatc tggcttataa attaaaatta attatttaat tgagaaatgg ttagggagaa
      121 cctacatggc cataagcatc aagggtgtta ataccggtgt tattcgcaag agtaataatt
      181 tcattgcact ggcgctgaaa attaaagaac ctcgcaataa agaatcgttg ttctttatgt
      241 ctgtcatgga actgcgcgac ctgctgattg cgctggaaag tcgtctgcat caaaagcata
      301 aactggatgc tgctgcacgc cttcagtatg agcaagctcg cgataaagtc attaagaaaa
      361 tggcagaaaa catcccagaa attctggttg atgagcttaa aaatgccgat attaatcgtc
      421 gggttaacac gctggaactt accgataatc aaggcgaaaa tcttaccttc gtattaacgc
      481 tacatgacgg tagcaaatgt gagttagtcg tcaatgagtt gcagattgaa atgctggcgc
      541 gagcaatcat tcatgcaatt aataatgcgg aaatgcgtga actggcatta cgtattacct
      601 cactgttaga tttcctgccg ttatacgacg ttgactgcca ggacaatggc aatctggaat
      661 atgacaccta ttcccaaccg gaatggaaac ataacttgtt cgaccattat ctggcggtgc
      721 tttaccgctt taaagatgaa agtggcaaag aacaatttag cggcgcagtg gtgaaaacgc
      781 gtgaagcaac gccgggtaaa gaaatagagg cgatcactcg ccgaatgctt gatttcagcc
      841 cacgactgaa aaaactcgcg ggtgtgccat gtcaggtcta tgttcgcacc gtagcggcga
      901 ataacgccca accactaacc caggatcaat gcttacgcgc gctgcatcac ttacgtgtac
      961 aatccaccag caaaactgca ccacaggcga aataatctca tctttgctaa tgcgaggctg
     1021 gagccatcca gcctgaaatg actgataacg tgatcgttgg tcatgccgga tgcggcgtaa
     1081 acgccttatc cggcctacaa attctcaagc attcaatcag ttgcaatgcc tgctgtaggc
     1141 ctgataagcg aagcgcatca ggcaataata cgtttgtcac ccagcctctg gatcacatca
     1201 gttcgggcac ttataaacct gaccgataat ctggctatcc gtcgggacaa aactggacaa
     1261 cattccctgc gacgggctgc tgatgccata aatcacgtta ccgcccattg cagccgcctg
     1321 gttgcgcaga tcgtttgctg cgccgcgcat agaaccgccc tcttctccgt gttgcccgga
     1381 aagccagttg ctttgcttac ctgtcgcagt accaatcagc tggcactctg cgcctggctg
     1441 ctcgtccaca atgcgtacac tctgaccggc agcactcaat tcgttgctgg agctacaacc
     1501 cgccatcagt agcgcggcac cgacaatccc tgctaagtat tttacgtgca tgttattccc
     1561 cataatcaat gagctggacg ccactcgcgt catccgtgtc tgaatcttat actagaaaga
     1621 tgaccaaaag aaaaaccccc agacatttct gcccgggggt ttgtttattt ctgcgaagtg
     1681 cagggcaatt acatcatgcc gcccatgcca cccatgccgc ccataccgcc agcagcgcct
     1741 aagtcagctg catcgttttt cggcaggtcg gtaaccatgc attcggtggt gatcatcagg
     1801 ccagccacag aagctgcgta ctgcagagca gaacgggtta ctttggttgg gtccaggata
     1861 cccatgtcga tcatgttgcc gtattcttcg gttgctgcgt tgtaaccgta gttgccgtcg
     1921 ccgcctttaa cggtgttagc aacaacagac ggttcttcgc cgcagttcag gacgatctga
     1981 cgcagcggag cttccattgc acgcagtgca actttgatac ccacgttctg gtcttcgttc
     2041 tgaccacgca ggtcagccag tttagacgct acgcggatca gcgcaacacc accaccagca
     2101 accacgcctt cttctaccgc agcacgggtc gcgtgcaggg catcttcaac gcgtgctttt
     2161 ttctctttca tttcaacttc ggtagcagca cctactttga taactgcaac gccgcctgcc
     2221 agtttcgcta cgcgctcctg cagtttttca cggtcgtagt cagaagttgc ttcttcaatc
     2281 tgctgacgga tctgagcaac acggccctgg attgcagctt cttcacccac gccatcgatg
     2341 atagtggtgg tgtctttgtt gatcacaaca cgtttagcct gacccaggtc ttccagggtt
     2401 gctttttcca gctccatacc gatctcttca gagattacgg taccgccagt cagggttgcg
     2461 atatcctgca gcatagcttt acgacgatcg ccgaagcccg gagctttaac cgcagcaact
     2521 ttcacgatgc cacgcatggt gttaacaacc agagttgcta gcgcttcgcc ttcaacatct
     2581 tcagcgatga tcagcagcgg tttgcctgct ttggcaacag cttccagaac cggcagcatt
     2641 tcgcggatgt tggagatttt cttgtcagcc agcaggatga acgggctttc cagttctact
     2701 gcgccagttt ccggcttgtt gatgaagtaa ggagacaggt agccacggtc gaactgcata
     2761 ccttcaacca cgtccagttc gtcctgcaga ccggtaccgt cttcaacggt gataacgcct
     2821 tctttaccga ctttgtccat cgcttcagcg atcagtttac ctacggtttc gtcggagtta
     2881 gcggagatgg taccaacctg agcaatcgct ttagaatcag agcacggtac ggacagcgct
     2941 ttcagttctt caactgcagc ggtaaccgct ttgtcgatac cacgtttcag gtccatcggg
     3001 ttcatgcccg cagcaacagc tttcagacct tcagtgatga tagcctgagc cagtacggtt
     3061 gcagtggtgg taccgtcgcc tgcagcgtcg ttcgctttgg aggcaacttc tttcaccatc
     3121 tgcgcaccca tattttcgaa cttgtcttcc agttcgattt cacgagcaac ggaaacacca
     3181 tctttggtga tggtcggtgc accgaaagat ttatccagaa ctacgttacg gccttttgga
     3241 ccgagggtaa ctttcactgc atctgccagt acgtttacgc cgcgcagcat tttcacacga
     3301 gcgtcgttac cgaattttac gtctttagct gccattatct ttattcctta aattcgtatg
     3361 ttcagtgtcg tgcgcggatt acgcttcaac aattgccaga atgtcgcttt cggacatgat
     3421 caacacttct tcattgtcga tcttctcgga tttcacaccg tagccatcgt tgaaaataac
     3481 gatgtcgcca actttcacgt ccagcggctt cacttcgcca ttttcaagga tacggccatt
     3541 gccgacagcc agcacttcgc cgcgggtgga tttagccgct gcagagccgg tcagaacgat
     3601 gccgccagca gatttagttt caacttcttt acgcttgacg atcacgcgat catgcaatgg
     3661 acgaatattc attgataact ctcctttgag aaagtccgta tctgttatgg gtgacgccgg
     3721 agcttacgtg gtttcccggc tggtgaccag agaaatgggg atgaggcttc gccccttcaa
     3781 gggggaaaaa gaaaaaaaat tctgatcagc acaaaatcgg gtgaaaaccc tgattcacct
     3841 cacatttcat cgcaatttct tcatcgccgt cataagcgaa tctgattgtg ctaccatcga
     3901 aaatctacgc agttgcccaa aatttgggcg caatcggata tcaacccatg agtggactca
     3961 aacaagaact ggggctggcc cagggcattg gcctgctatc gacgtcatta ttaggcactg
     4021 gcgtgtttgc cgttcctgcg ttagctgcac tggtagcagg caataacagc ctgtgggcgt
     4081 ggcccgtttt gattatctta gtgttcccga ttgcgattgt gtttgcgatt ctgggtcgcc
     4141 actatcccag cgcaggcggc gtcgcgcact tcgtcggtat ggcgtttggt tcgcggcttg
     4201 agcgagtcac cggctggctg tttttatcgg tcattcccgt gggtttgcct gccgcactac
     4261 aaattgccgc cggattcggc caagcaatgt ttggctggca tagctggcaa ctgttgttgg
     4321 cagaactcgg tacgctggcg ttggtgtggt atatcggtac tctcggtgcc agttccagtg
     4381 ctaatctaca aaccgttatt gccggactta tcgtcgcact gattgtcgct atctggtggg
     4441 cgggcgatat caaacctgcg agtatcccct tccccgcgcc aggaaatatc gaacttaccg
     4501 ggttatttgc tgcgttatca gtgatgttct ggtgttttgt cggtctggaa gcatttgccc
     4561 atcttgcctc ggaatttaaa aatccagagc gtgattttcc tcgtgctttg atgattggtc
     4621 tgctgctggc aggattagtc tactggggct gtacggtagt cgtcttacac ttcgacgcct
     4681 atggtgaaca aatggcggcg gcagcatcgc ttccaaaaat tgtagtacag ttgttcggtg
     4741 taggagcgtt atggattgcc tgcgtaattg gctatctggc ctgctttgcc agtcttaaca
     4801 tttatataca gagcttcgcc cgcctggtct ggtcgcaggc gcaacataat cctgaccact
     4861 acctggcacg cctctcttct cgccatatcc cgaataatgc cctcaatgcg gtgcttggct
     4921 gctgcgtggt gagcacgttg gtaattcatg ccttagagat caatctggac gctcttatta
     4981 tttatgccaa tggcatcttt attatgattt atctgttatg catgctggca ggctgtaaat
     5041 tattgcaagg tcgttatcga ctactggcgg tggttggcgg gctgttatgc gttctgttac
     5101 tggcaatggt cggctggaaa agtctctatg cgctgatcat gctggtgggg ttatggctgt
     5161 ttctgccaaa acgaaaaacg ccggaaaatg gcataaccac ataatccggc gtttcgacat
     5221 taatcctggc gatcgtcttt atgatcaagg cggtcgcggt catcatcctt tcgctgatac
     5281 tcaccatcaa aagtattacc gccaccggtc ccggcgctaa aaccgccgcc tggcatgcga
     5341 gaaaagcgca aatgtggcat caacttcact gtcagatgct tttgcaccgg cggcaataaa
     5401 agcagaagac cgaggaagtc ggtgaaaaag cccggtaata aaagcagcaa accggcaata
     5461 atcagcgaaa cacttttaat catctccgcc gcagggtttt cacccgccgc cattttttgc
     5521 tgcatcagca cgaaattctt aaagccctgg ttacgtacca gtgacatacc gataactgac
     5581 gtgaatataa ccagcacgag ggtcagcaat acccccaata catgggcaac ctgaataaag
     5641 attgaaatct caatatagac ataaaggaaa atggcaataa aaggtaacca gcgcaaaggt
     5701 ttctcctgta atagcagccg gttaaacccg gctacctgaa tgggttgcga atcgccttca
     5761 gcttatattg tggtcattag caaaatttca agatgtttgc gcaactattt ttggtagtaa
     5821 tcccaaagcg gtgatctatt tcacaaatta ataattaagg ggtaaaaacc gacacttaaa
     5881 gtgatccaga ttacggtaga aatcctcaag cagcatatga tctcgggtat tcggtcgatg
     5941 caggggataa tcgtcggtcg aaaaacattc gaaaccacat atattctgtg tgtttaaagc
     6001 aaatcattgg cagcttgaaa aagaaggttc acatgtcaaa caacattcgt atcgaagaag
     6061 atctgttggg taccagggaa gttccagctg atgcctacta tggtgttcac actctgagag
     6121 cgattgaaaa cttctatatc agcaacaaca aaatcagtga tattcctgaa tttgttcgcg
     6181 gtatggtaat ggttaaaaaa gccgcagcca tggcaaacaa agagctgcaa accattccta
     6241 aaagtgtagc gaatgccatc attgccgcat gtgatgaagt cctgaacaac ggaaaatgca
     6301 tggatcagtt cccggtagac gtctaccagg gcggcgcagg tacttccgta aacatgaaca
     6361 ccaacgaagt gctggccaat atcggtctgg aactgatggg tcaccagaaa ggtgaatatc
     6421 agtacctgaa cccgaacgac catgttaaca aatgtcagtc cactaacgac gcctacccga
     6481 ccggtttccg tatcgcagtt tactcttccc tgattaagct ggtagatgcg attaaccaac
     6541 tgcgtgaagg ctttgaacgt aaagctgtcg aattccagga catcctgaaa atgggtcgta
     6601 cccagctgca ggacgcagta ccgatgaccc tcggtcagga attccgcgct ttcagcatcc
     6661 tgctgaaaga agaagtgaaa aacatccaac gtaccgctga actgctgctg gaagttaacc
     6721 ttggcgcaac agcaatcggt actggtctga acacgccgaa agagtactct ccgctggcag
     6781 tgaaaaaact ggctgaagtc actggcttcc catgcgtacc ggctgaagac ctgatcgaag
     6841 cgacctctga ctgcggcgct tatgttatgg ttcacggcgc gctgaaacgc ctggctgtga
     6901 agatgtccaa aatctgtaac gacctgcgct tgctctcttc tggtccacgt gccggcctga
     6961 acgagatcaa cctgccggaa ctgcaggcgg gctcttccat catgccagct aaagtaaacc
     7021 cggttgttcc ggaagtggtt aaccaggtat gcttcaaagt catcggtaac gacaccactg
     7081 ttaccatggc agcagaagca ggtcagctgc agttgaacgt tatggaaccg gtcattggcc
     7141 aggccatgtt cgaatccgtt cacattctga ccaacgcttg ctacaacctg ctggaaaaat
     7201 gcattaacgg catcactgct aacaaagaag tgtgcgaagg ttacgtttac aactctatcg
     7261 gtatcgttac ttacctgaac ccgttcatcg gtcaccacaa cggtgacatc gtgggtaaaa
     7321 tctgtgccga aaccggtaag agtgtacgtg aagtcgttct ggaacgcggt ctgttgactg
     7381 aagcggaact tgacgatatt ttctccgtac agaatctgat gcacccggct tacaaagcaa
     7441 aacgctatac tgatgaaagc gaacagtaat cgtacagggt agtacaaata aaaaaggcac
     7501 gtcagatgac gtgccttttt tcttgtgagc agtaacttaa aaataacaat ctaatatcaa
     7561 cttgttaaaa aacaaggaag gctaatatgc tagttgtaga gctcatcata gttttgctgg
     7621 cgatcttctt gggcgccaga ttggggggaa taggtattgg ttttgcaggc ggattggggg
     7681 tgctggttct tgccgctatt ggcgttaaac ccggtaacat cccgttcgat gtcatttcca
     7741 ttatcatggc ggttatcgcc gctatttctg ccatgcaggt tgctggcggt ctggactatc
     7801 tggttcatca gacagaaaag ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac
     7861 cgatcgtgac ctatttcctg actatctttg ctggtactgg caacatctct ctggcgacac
     7921 tgccagttat cgctgaagtt gcgaaggaac aaggcgttaa accttgccgt ccgctctcta
     7981 ctgcagtggt atccgcgcag attgcgatca ccgcatcgcc aatctcagcg gcagtggttt
     8041 acatgtcttc cgtgatggaa ggtcatggca tcagctacct ccatctgctc tccgtggtca
     8101 tcccgtccac cctgctggcg gttctggtga tgtccttcct ggtcactatg ctgttcaact
     8161 ccaaactctc tgacgatccg atttatcgca agcgtctgga agagggcttg gttgaactgc
     8221 gcggtgaaaa gcagattgaa atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc
     8281 tgggcgtagt tggcgtggtt atctatgcaa tcatcaacag cccaagcatg ggtctggttg
     8341 aaaaaccgct gatgaacacc accaacgcaa tcctgatcat catgctcagc gttgcaactc
     8401 tgaccaccgt tatctgtaaa gtcgataccg acaacatcct caactccagc accttcaaag
     8461 caggtatgag cgcctgtatt tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt
     8521 ccaacaacat cgactggatc aaagataccg ctggtgaagt gattcagggt catccgtggc
     8581 tgctggccgt catcttcttc tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa
     8641 aagcactgat gccgatggct ctggcactga acgtttcacc gctgaccgct gttgcttctt
     8701 tcgctgcggt gtctggtctg ttcattctgc cgacctaccc gacgctggtt gctgcggtac
     8761 agatggatga cacgggtact acccgtatcg gtaaattcgt cttcaaccat ccgttcttca
     8821 tcccgggtac tctgggtgtt gccctggccg tttgcttcgg cttcgtgctg ggtagcttca
     8881 tgctgtaatg atcaatcgcg gggcgttcac gccccgcttt ctttcccgcc gactaacatc
     8941 cttcccccgt ccgttgtata gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg
     9001 tttatgcttg atgaaaaaag ttcgaatacc gcgtctgtcg tggtgctatg taccgcaccg
     9061 gatgaagcga cagcccagga tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc
     9121 gcgaccttga tccccggcgc tacctctctc tattactggg aaggtaagct ggagcaagaa
     9181 tacgaagtgc agatgatttt aaaaactacc gtatctcacc agcaggcact gctggaatgc
     9241 ctgaagtctc atcatccata tcaaaccccg gaacttctgg ttttacctgt tacacacgga
     9301 gacacagatt acctctcatg gctcaacgca tctttacgct gattctgcta ctttgcagca
     9361 cttccgtttt tgccggatta ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc
     9421 aagcctttgc ttttgatttt cagcaaaacc aacatgacct taatctgacc tggcagatca
     9481 aagacggtta ctacctctac cgtaaacaga tccgcattac gccggaacac gcgaaaattg
     9541 ccgacgagca gctgccgcaa ggcgtctggc atgaagatga gttttacggc aaaagcgaga
     9601 tttaccgcga tcggctgacg cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt
     9661 taactgtcac ctaccagggc tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa
     9721 ccgttccgtt aagcgaagtg gtcgccaaca acgcagcgtc acagcctgtg tctgtttcgc
     9781 agcaagagca gcacaccgcg caattgccct tttccgcgct ctgggcgttg ttgatcggta
     9841 ttggtatcgc ctttacgcca tgcgtgctgc caatgtaccc actgatttct ggcatcgtgc
     9901 tgggtggtaa acagcggctc tccactgcca gagcattgtt gctgaccttt atttatgtgc
     9961 aggggatggc actgacttac acggcgctgg gtctggtagt tgccgccgca gggttacagt
    10021 tccaggcggc gctacagcac ccatacgtgc tcattggcct cgccatcgtc tttaccttgc
    10081 tggcgatgtc aatgtttggc ttgtttactc tacaactccc ctcttcgctg caaacacgtc
    10141 tcacgttgat gagcaatcgc caacagggcg gctcacctgg cggtgtgttt gttatggggg
    10201 cgattgccgg actgatctgt tcaccatgca ccaccgcacc gcttagcgcg attctgctgt
    10261 atatcgccca aagcgggaac atgtggctgg gcggcggcac gctttatctc tatgcgttgg
    10321 gcatgggcct gccgctgatg ctaattaccg tctttggtaa ccgcttgctg ccgaaaagcg
    10381 gcccgtggat ggaacaagtc aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct
    10441 tcctgctgga gcgagtgatt ggtgatgtat ggggattacg cttgtggtcg gcgctgggtg
    10501 tcgcattctt tggctgggcc tttatcacca gcctacaggc taaacgcggc tggatgcgcg
    10561 tggtgcaaat aatcctgctg gcagcggcat tggttagcgt gcgcccactt caggattggg
    10621 catttggtgc gacgcatacc gcgcaaactc aaacgcatct caactttaca caaatcaaaa
    10681 cggtagatga gttaaatcag gcgctcgttg aagccaaagg caaaccggtg atgttagatc
    10741 tttatgccga ctggtgcgtc gcctgtaaag agtttgagaa atacaccttc agcgacccgc
    10801 aggtgcaaaa agcgttagca gacacggtct tacttcaggc caacgtcacg gccaacgacg
    10861 cacaagatgt ggcgctgtta aagcatctta atgtccttgg cctaccgaca attctctttt
    10921 ttgacggaca aggccaggag catccacaag cacgcgtcac gggctttatg gatgctgaaa
    10981 ccttcagcgc acatttgcgc gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg
    11041 aggaaaatag cgtgcaacgt gaagatgtac tgggagaagc cctgaaatta ttagaattac
    11101 aagggattgc caacaccacg ctggagatgg ttgctgaacg tgtggattat ccactggacg
    11161 agctacgccg cttctggcca gacaaagagg caatcctcta cgatgcgctg cgttatctta
    11221 gccaacagat agatgtctgg cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac
    11281 aaaagctgct ggcacgttat caggcgttat cggagtgcgt taaaaacaac cgctatccgg
    11341 gctgtctatt tatcgctgcc tgtacgtttt atcccgatcc tggccaccct attcatcaac
    11401 tggccgatca gcaaaaaagc gctgcctacg atttcaccca cgaactgtta accacgctgg
    11461 aagttgacga tccggcgatg gtagcaaagc agatggaact ggtgctggaa ggctgtttaa
    11521 gccgaatgct ggtgaatcgt agccaggcgg atgtcgacac tgcacatcgg ctggcggaag
    11581 atatcctgcg cttcgcccgc tgccgccagg gtggtgcatt gacctgacag aaacacagaa
    11641 aagaagcgat ttgccgcaat cttaagcagt tgaatcgctt ttactgaaat taggttgacg
    11701 aaatgtgcag attacggttt aatgcgcccc gttgcccgga tagctcagtc ggtagagcag
    11761 gggattgaaa atccccgtgt ccttggttcg attccgagtc cgggcaccaa attcatatca
    11821 acggacctcc acggaggtcc gtttttcgtt tcaggacgcc acgatttaag cgttctgcct
    11881 ccaaatcaat tctaccgaac tcaaccagat tctccccaca tcaccagcaa tttgcgagca
    11941 tatcccaatt tgggaaaatt tgttcctgag ctatagcact aactgacgtg aaatgtcgtg
    12001 cggccccgtg atgctgttga acgtcaaatg acgtcatcag gagcgtaacg tatccataaa
    12061 gcacaacacc ggtctgaacg ccgactgatg agattttctg aatgagaaca aagagaaatg
    12121 tgtcagtccg cttgctcatg caaagactaa caa
//
LOCUS       DAEEUE010000110        12144 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.110, whole genome shotgun sequence.
ACCESSION   DAEEUE010000110 DAEEUE010000000
VERSION     DAEEUE010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12144
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.110"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..561
                     /locus_tag="KLK84_003354"
     CDS             <1..561
                     /locus_tag="KLK84_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE2010558.1"
                     /translation="LNSLDTRMVPQASAWAINRVAQKAVSVATRQVAGNTVAGDNQVK
                     GIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEGLRVGKYFFRG
                     AFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIPMSGPLTQAFEDAR
                     DRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            558..959
                     /locus_tag="KLK84_003355"
     CDS             558..959
                     /locus_tag="KLK84_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE2010559.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLNPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            970..1710
                     /locus_tag="KLK84_003356"
     CDS             970..1710
                     /locus_tag="KLK84_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HBE2010560.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            1769..2155
                     /locus_tag="KLK84_003357"
     CDS             1769..2155
                     /locus_tag="KLK84_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HBE2010561.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            2164..2493
                     /locus_tag="KLK84_003358"
     CDS             2164..2493
                     /locus_tag="KLK84_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HBE2010562.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            2465..5203
                     /locus_tag="KLK84_003359"
     CDS             2465..5203
                     /locus_tag="KLK84_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBE2010563.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARAAQLGVSSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGN
                     FGALRGSGITLANRAGWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFN
                     RQLALTGHYAGVTVGQLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKA
                     AAQMERSVGQSVSDTINQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSS
                     DAARIAMSALAEETGKRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQ
                     IDALQEKIQRAKKYPWTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQK
                     RRNAENAALNRMNETEAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTR
                     ATRNDEATRLLLQYSQQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDG
                     KKLTADEKSVLARKNELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERAL
                     REQHNLDIATAGMGDKQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEE
                     VLKGSLKRQLNENKRYWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFS
                     SAGNALATFCTTGKLNFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGV
                     YQSADLSRYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSG
                     MVMFAPQYNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            5203..5532
                     /locus_tag="KLK84_003360"
     CDS             5203..5532
                     /locus_tag="KLK84_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE2010564.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            5532..6230
                     /locus_tag="KLK84_003361"
     CDS             5532..6230
                     /locus_tag="KLK84_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HBE2010565.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            6235..6978
                     /locus_tag="KLK84_003362"
     CDS             6235..6978
                     /locus_tag="KLK84_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE2010566.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6915..7517
                     /locus_tag="KLK84_003363"
     CDS             6915..7517
                     /locus_tag="KLK84_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HBE2010567.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            7578..11058
                     /locus_tag="KLK84_003364"
                     /pseudo
     CDS             7578..11058
                     /locus_tag="KLK84_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
     gene            11126..11725
                     /locus_tag="KLK84_003365"
     CDS             11126..11725
                     /locus_tag="KLK84_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HBE2010568.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            11747..>12144
                     /locus_tag="KLK84_003366"
     CDS             11747..>12144
                     /locus_tag="KLK84_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HBE2010569.1"
                     /translation="MTARWFRRAFCGVNMAVKISGVLKDGAGKPVVNCAIELRARRTS
                     PTVVAHVVATCVTDNNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNA
                     FLNAPKDGDLRPEVMKRFEEMVAQAQQSAGA"
BASE COUNT         2846 a         2873 c         3884 g         2541 t
ORIGIN      
        1 ctgaacagcc ttgatacccg tatggtgcca caggccagcg catgggcgat aaaccgtgtg
       61 gcacagaaag cggtctcggt tgccacccgg caggttgccg ggaataccgt tgcgggcgat
      121 aaccaggtga aagggatccc cctgaaactg gtacgtcagc gtgtccgggt gtttaaagcc
      181 agtccgtcag gaaaaatgac ggccaggatc cgcgttaacc ggggcaatct gcccgccatc
      241 aaactgaaca caacacggcg gcgtgctggt gaaggactga gagtgggaaa atactttttc
      301 cggggggcat ttgttcagca actggcgaat ggccgctggc atgttctgag gtgtcttcct
      361 gaagcgcgtt ttgcaacagg gcatgaccat cagggcaggc taagaaaaaa tcgtcttcct
      421 gtggaggtgg tgaaaatccc gatgtccgga ccgctgacac aggcatttga agatgcccgc
      481 gaccgcatca ttgctgcgga aatgccgaaa cagctggggt atgccctgaa acaacaactg
      541 aggttacatc tgagtaaatg aaccgacaca cacaaatccg tcagagcgtg ctggcacgcc
      601 ttcgggaaca gtgtggagac agcgccacgt tttttgacgg gcttccggca tttattgatg
      661 cgcaggaact gcctgccgtg gcggtgtggc tgagtgatgc tcagtacacc ggaaaaatga
      721 cggatgaaga tgactggcag gctgttctgc atattgccgt cttcatccgg gcacaggcac
      781 cggattcaga gctggatatg tggatggaga gcaccatttt cccggccctg aatgatgtac
      841 cggcactttc cggactcatc gacaccctga acccactcgg ttttaactat caacgtgata
      901 atgagatggc tacctgggcg atggcggaaa tcacgtacca gatcacgtac acgaattaag
      961 gaggtggtaa tgaccacacc aaatccactg gcaaagacga aaggtgcggg gacgacgttc
     1021 tggatgtata ccggcaacgg cgatgcgttt gcgaaccctt tgtcggacac tgactggctg
     1081 cgtcttgcga tggtgaagga tctgcaacct ggcgaaatga ccgctgatgc agaagatgac
     1141 acttatctcg atgatgaaga tgcagactgg aaaacgacaa cccaggggca gaaatccgtt
     1201 ggtgatactt cggcgacgct ggcctggcgt ccgggtgaca gcgggcagaa aaaactggtt
     1261 cagttgttcg actccggtga agtctgcgcg tttcgtatca aatatcccaa cggcactgtt
     1321 gatgttttcc gcggctggct gagctcactg ggtaaaacca ttgcctcaaa agacgtgatg
     1381 acccgcactg tgaaaatcag cggtgtgggg cgtccgtatc tggcagagga aggcactgaa
     1441 accgtgggtg ttaccggact gacggtggca ccggcatctg tcagtgtcaa agcgggagca
     1501 accaccacac tgacctttac agtaaaacct gacggggcca gtgacaaagc gatcagtgtg
     1561 cattcgtcag atcctcagac tgcttcggtg accctgagcg ggcttgtggc cacggtgaaa
     1621 ggcgtgaagc agggcagtgt cagcatcgtg ggcatgacct ctgacggaga gtttgtggca
     1681 gtggctgcgg tgaccgtcag cgcaccataa caggacgata ctcatcattg ccccggttat
     1741 ccggggcttt ttgcatccgg agaacatgat gtttctgaaa caggatacgt ttaattatga
     1801 aaaacagtcc gtggtgctca gtgagctgtc cgggctgcag agaattgaat atctgacgtt
     1861 tgttcagcag cgaacggcaa agtttgatgc acaggaggga gaactgccgg aggctgaacg
     1921 acagattgct tttctgcgta tgggaatgga tatcaatgcc tggctggttt cccgctcact
     1981 gtggaatgct gagcagtctc aggatgttga gacgctttgc gcatccatta tgacaacatg
     2041 gtcgtatgat gcgctgggcg cgggggcgga gagggttctg tcgctgagcg gtatggggac
     2101 cattgagaat gccggggatg atgatcatga ggcgctgacg ccggaaaagt cctgacgcgg
     2161 gaaatgcagt ttgtcatgcg gcttgcccgg gagttccggc gggcagactg gcggcggatg
     2221 ctgtcggata tgtcggccac tgagcttggt gagtggggcg attatttccg gatgcagagc
     2281 ttcagtgatg tgtggatgga tgcgcagttt gcctcgctga aggcattgat cgtgagaatg
     2341 gtgtccggca gcagcgatgc tgcggtggct gatttcagcc ttttaccgga agagaacggg
     2401 ataccggagc gaacggacga agaactgatg catcttgggg aaggtatttc cggaggtgtg
     2461 cgttatggac cagatagcca acctggtcat tgatttgggg attgatgcgg cagagtttaa
     2521 aaatgaaatt ccccgtatca aaaaccttct gaatggtgca gccagcgatg cagaacggtc
     2581 ttctgcccgt atgcagcgtt ttatggagcg tcagactcag gcggcccggc agacaatgca
     2641 ggcggcttct tcggctgcaa cagcagcatc agcccatgcg cagacggtgg agaagaatgc
     2701 acgggctcat gaacgcatgg cccgtgaggt ggaacaaacc cgtctgcgcg tggatgccct
     2761 gaatcagaaa atgcgcgagg aacaggcgca ggccagggcg gctcagctgg gggtaagcag
     2821 tgctgcagaa gtgtatatcc gcaaaatgga gcgggcagga aaagccacac attcgctggg
     2881 gctgaaaagt gcggcagccc ggcgggagct gggggtgtta atcagtcaga tggcgcgcgg
     2941 caattttggt gcgctgaggg gatccgggat aacgctggct aaccgtgccg gatggacagg
     3001 cgcactgatg tcgccgaaag gcatgatgac tggcggcgtt attggcggac ttgtcgcggc
     3061 ggtcctgggt ctgggtaaag cctggcatga cggccggaag gagggcgaga aatttaaccg
     3121 tcagctggcg ctgacgggac attatgccgg tgtcactgtc gggcagttgt ggaaactcag
     3181 ccgggccata tccgggaatg gtatcacgca acatgcggca gccggtgcgc tggcacaggt
     3241 ggtgggcagt ggggcatttc atggaaacga tattggtatg gtggcgaaag ctgccgcaca
     3301 gatggagcga tcggttggcc agtcggtcag cgataccata aatcagttta agcggctgaa
     3361 ggatgatcct gtaaatgccg cgaaggcgct ggacaatgcg ctgcattttt tgaatgccac
     3421 tcagcttgag cagatacgcg tccttgggga gcaggggcgg tccagtgatg cggcccggat
     3481 cgccatgtct gcgctggcag aggaaaccgg taaacgcaca tctgatattg ataataatct
     3541 caatgcgctg ggtagtacgc tgcaaacctt gtctgactgg tggaagcagt tctgggatgc
     3601 ggccatgaat attggtcgtg aagactcgct ggatgcgcag attgatgcgt tacaggaaaa
     3661 aattcagcgc gcgaaaaaat atccgtggac aaacgcctcc acacaggtgg agtacgatca
     3721 gcagcgtctt aacgatcttc aggagaaaaa acgccggaag gatttgcagg atgcaaaagc
     3781 gcaggcagaa cggaattacc aggagcaaca gaaacgccgg aatgctgaaa atgccgcgct
     3841 gaaccggatg aatgaaacgg aagctgcacg acatcagcgg gaaattgcgc gtattaatgc
     3901 catgcagtac gccgaccagg ctgtcaggga tgcggcgata cagcgtgaaa atgaacgtta
     3961 cgaaaaagcc attaagaaaa atacacgggc aacccgtaat gatgaggcca cccggttatt
     4021 gctgcagtac agtcagcagc aggcacaggt ggaagggcag attgctgctg ccagacagtc
     4081 agcaggcatt gccactgaaa ggatgactga agcgcataaa cagcttctgg ccctgcagca
     4141 gcgcatcagc gacctggacg ggaaaaagct gacggcagat gaaaagagtg tgctggcccg
     4201 taagaatgag ctgattcagg cgctgacgct gctggatgtg aaacagcagg agctgcagaa
     4261 acagacagcg cttaacgacc tgagaaaaaa aacggttcag ctgaccagcc agctggcaga
     4321 caaagaacgt gcactgcgtg agcagcacaa tctggatatt gccactgcag gtatggggga
     4381 taagcagcgg cagcgctacc aggcacagtt gcgcatccgg caggaatacc ggcaacagtt
     4441 gcaacagctt gagaatgaca gtcgccagaa aggcacttac gggacggagg actaccggag
     4501 ggctgaggag gtgctgaagg ggagcctgaa gcgacaactg aatgaaaaca aacgctactg
     4561 gcaggaactg gaagtggcgc agggcgactg gaaaaacggt gccatgcggg cgtttcagaa
     4621 ttttacggcg gatgcggata atgcggcggg aacggcagaa cagatgttta cagtggcatt
     4681 cagcagtgcc ggtaatgcac tggcgacatt ctgtaccacc ggtaagctga attttaaatc
     4741 cttcacctct tccctgttgt cagatatggc cagaattatg gcacagatgg ccatgatgca
     4801 ggcggtaaag ggcgtcggtt ctttattcgg cttcacgact aatgctgatg gcggtgttta
     4861 ccagtctgct gatttgagtc gctacagtgg cacggtggtt aaccgtccga cgttttttgc
     4921 ttttgcaaaa ggcgcgggtg tgatggggga agcgggacct gaagccattc tgccactgcg
     4981 tcgcggtgct gatggtaagc tgggggttgt ggcggatact ggtggttcag gcatggtgat
     5041 gtttgccccg cagtacaaca ttgagatcaa taacgacggc acgaacgggc agataggtcc
     5101 ggctgccctg aaggtggttt atgacctcgg gaaaaaagcg gccgcggact ttatgcaaca
     5161 gcaggcccgt gatggtggcc ggttaagtgg agcataccgg taatggagac gtttcactgg
     5221 aaagtgcgcc cggatatgaa tgtggtatca gagccgaaag tggtgacagt gaagctgggc
     5281 gatggttatg aacagcgtcg tgcggcggga ctgaataacc agttgtcgac ttacagcgtg
     5341 acgatacgtg ttcgtaaagg tgaacaccca tctttaaaag cctttctgga acggcacggt
     5401 ggcgtccgcg catttcagtg gacgccacct tatgactgga aactgatcag ggtggtttgt
     5461 cgtaaatggt cggcaagcgt gggggcgtta tgggtgactg taacggccga ttttgaacag
     5521 gtggtgaact gatgcaggat atccggcagg aaacactgaa tgaatgcacc cgtgcggagc
     5581 agtcagcccg ggtggagctc tgggaaatcg atctgacaga ggtcggtggt gagcgttatt
     5641 ttttctgtaa tgagcagaac gaaaaaggtg agccggtcac ctggcagggg cggcagtatc
     5701 aggcataccc cattcagggg tcggggtttg aactgaacgg caggggctgt gctgcccgtc
     5761 cgacactgac ggtctctaac ctgcacggca tggtcaccgg gatggcggaa gatctgcaga
     5821 gtctggtcgg cggaacggtg gtccggcgta aggtttacgc ccgttttctg gatgcggtga
     5881 acttcgtcaa cggaaacagc gacgccgatc cggaacagga ggtgatcagc cgctggcgca
     5941 tcgagcagtg cagcgaactg agcgcggtca gtgcctcttt tgtgctggcc acaccaacgg
     6001 agacggatgg cgcggttttc ccggggcgta tcatgctggc gaatacctgt atgtggactt
     6061 accgttctga tgagtgtggt tacacgggca gggcagtggc tgacgagttc gacaaaccaa
     6121 caacggatat ccggaaggac aaatgcagca agtgtatgcg cgggtgtgag ttgcgcaaca
     6181 ataccggtaa tttcggcggt ttcctttcca tcaataaact ttctcagtaa atccatgaca
     6241 cagacagaat cagcgattct ggcgcacgcc cggcgatgtg cgccagcgga gtcgtgtggc
     6301 ttcgtggtga gaacgccgga gggagacagg tatcttccca gcgagaatat ctccggtgag
     6361 ccggaggaac ggttccggat ggctccggag gactggctgc gggcacaaat gcagggtgag
     6421 attgtggcac tggtccacag tcatcccggt ggtctgccct ggctgagtga ggctgaccgg
     6481 cggctgcagg tgcagagtga tttgccgtgg tggctggtct gccggggggc gattcacaag
     6541 ttccgctgtg tgccgcatct caccgggcgg cgctttgagc acggggtgac ggactgttac
     6601 acgctgttcc gggatgctta tcatctggcg gggattgaga tgccggattt tcatcgcggg
     6661 gatgactggt ggcgtcacgg tcagaatctc tatctggata atctggaggc cacagggctg
     6721 tatcaggtgc cgttgtcatc agcacaaccg ggcgatgtgc tgctgtgctg ttttggttca
     6781 tcggtgccga atcatgccgc catttactgc ggcgatggtg agctgctgca ccatattcct
     6841 gaacaactga gtaaacgaga gaggtatacc gacaaatggc agcgacgcac acactccctc
     6901 tggcgtcacc gggaatggca cgcatctgcc tttacgggga tttgcaacga tttggccgcc
     6961 gcatcgacct tcgtgtgaaa acgggggctg aagccatccg cgcactggcc acacagctcc
     7021 cggcgtttcg tcagaaactg agtgacggct ggtatcaggt gcgcattgcc gggcgtgatg
     7081 caggtgaaac cgaattgtct gcccgtctta atgagccgct ggcaaatggt gcagtgatcc
     7141 acatagtacc gcgtctggtg ggagctaaaa gtggcggtgt gtttcaggcg gtgctggggg
     7201 cagctgttat ggcggttgct atatggatgc cgggggtagg aattatggcg agtaatctgc
     7261 tgttttctct cggtgccagt atgacgcttg gcggtgttgc acagatgctg gcaccgaaag
     7321 ccagaactcc ccgtacacag acaacggata acggcaaaca gaacacctgt ttctcctcac
     7381 tggataacat ggttgcccag ggcaatgttc tgcctgttct gtacggtgaa atgcgcgtgg
     7441 ggtcgcgggt ggtatctcag gagatcagca cggcagacga aggggatggt ggtcaggttg
     7501 tggtgattgg tcgctgatgc aaaatgtttc atgtgaaacc gcctgcgggc ggttttgtcg
     7561 tttatggagc gtgaggaatg ggtaaaggca gcagtaaggg gcataccccg cgcgaagcga
     7621 aggacaacct gaaatccacg cagttgctga gtgtgattga tgccatcagc gaagggccgg
     7681 ttgacggtcc ggtggatgga ttaaaaagcg tgctgctgaa cggtacgccg gtcctggata
     7741 gcgaggggaa gacaaacttt tccggtgtta cggtggtgtt ccgcgccggc gagcaggagc
     7801 agacaccgcc ggaggggttt gaatcttccg gctcagagac tgtgctgggt acggaagtga
     7861 aatacgacac gccgatcacc cggaccatca cgtcggcaaa cattgatcgt ctgcgcctga
     7921 ccttcggtgt gcaggcactg gtggaaacca cctcaaaggg ggaccggaat ccgtcggaag
     7981 tccgcctgct ggttcagata cagcgtaacg gtggctgggt gacggaaaaa gacatcacca
     8041 ttaagggcaa aaccacttcg cagtatctgg cctcggtggt ggtgggtaac ctgccgccgc
     8101 gcccgtttaa tatccggatg cgcaggatga caccggacag caccacagac cagctgcaga
     8161 acaaaacgct ctggtcgtcg tacaccgaaa tcatcgatgt gaaacagtgc tacccgaaca
     8221 cggcgctggt cggcgtgcag gtggattcag agcagttcgg taaccagcag gtgagtcgca
     8281 attatcatct tcgcgggcgc attctgcagg tgccgtcgaa ctataacccg cagacgcggc
     8341 aatacagcgg tatctgggac ggaacgctta agccagcata cagcaacaac atggcctggt
     8401 gtctgtggga catgctcact catccgcgct acggcatggg gaaacgtctt ggtgcggcag
     8461 atgtggacaa atgggcgctg tatgtcatcg gccagtactg cgaccagtca gtgccggacg
     8521 gatttggcgg cacggagccg cgcatcacct gtaacgctta cctgaccaca cagcgtaagg
     8581 cgtgggatgt tctcagtgat ttctgctcgg cgatgcgctg tatgccggta tggaacgggc
     8641 agacgctgac gttcgtgcag gaccgaccgt cggataaagt gtggacctat aaccgcagta
     8701 atgtggtgat gccggatgat ggcgcgccgt tccgctacag cttcagcgcc ctgaaggacc
     8761 gccataatgt cgttgaggtg aactggattg atccggacaa cggtcatgag acggcgacag
     8821 agcttgtgga ggacacgcag gccattgtcc gttacggtcg taacgtcacg aagatggatg
     8881 cctttggctg taccagccgg ggacaggcgc accgcgccgg gctgtggctg attaaaacgg
     8941 aactgctgga aacgcagacc gtggacttca gcgtcggcgc agaagggctt cgccatgtac
     9001 cgggtgatgt cattgaaatc tgcgatgatg attatgccgg tatcagcacc ggtggtcgcg
     9061 tactggcggt gaacagccag acccggacgc tgacgctcga ccgtgaaatc acgctgccgt
     9121 cctccggtac cacgctgata agcctggttg acggaaatgg caatccggtc agcgtggagg
     9181 ttcagtccgt caccgacggc gtgaaggtga aagtgagccg ggttcctgac ggcgttgcag
     9241 aatacagcgt gtgggggctg aagctgccga cgctgcgcca gcgcctgttc cgctgcgtga
     9301 gtatccgtga gaacgacgac ggcacgtatg ccatcaccgc cgtgcagcat gtgccggaaa
     9361 aagaggccat cgtggataac ggggcacact ttgacggtga ccggcgcggc acggtgaatg
     9421 gtgtcacgcc gccagcggtg cagcatctga ccgccgaagt caccgcagac agcggggaat
     9481 atcaggtgct ggcgcgatgg gacacgccga aggtggtgaa gggtgtgagc ttcctgcttc
     9541 gcctgaccgt ggcagcggac gacggcagtg agcggctggt cagcacagcc aggacgacgg
     9601 aaaccacata ccgcttcagg cagctggcgc tggggaatta cagtctgaca gtccgggcgg
     9661 taaatgcccg ggggcagcag ggcgatccgg cgtcggtatc gttccggatt gcggcaccgg
     9721 cagcgcctgt cactattgaa ctgataccgg ggtattttca gataacggtg gtcccgaaac
     9781 tggctgtata tgacccgacg gtgcagtttg agttctggtt ttcggaaaag cggattgcgg
     9841 atatcaggca ggttgaaacc agcgcccgct atcttggcac ggcgctgtac tggatagccg
     9901 ccagtatcaa tatcaggccg ggccatgatt attattttta cgttcgcagt gtgaacaccg
     9961 ttggcaaatc ggcatttgtg gaggccgtcg gtcgggcgag cgatgatgcg gaaggttatc
    10021 tgagttttta taaagggttg atcaataaaa cgcatctcgg caaggagttg tggacgcaga
    10081 ttgataacgg tcagcttgcg ccggacctga ctgaaatcag gacgtccatt acgaatgtca
    10141 gcaatgaaat cacgcaaacc gtcaataaaa aactggaaaa tcagagtgct gcaatccagc
    10201 agatacagaa agttcaggtt gatacaaata ataacctgaa cagcatgtgg gctgtgaagc
    10261 tgcaacagat gaaggacgga cgcctttata ttgcgtggta tcggtgccgg tattgagaat
    10321 acgccagcag gaatgcagag tcaggtgctg ctggcggcag acaggattgc gatgattaat
    10381 cctgcgaatg gcaacacaaa gccgatgttt gttggtcagg gcgatcagat attcatgaac
    10441 gacgtgttcc tgaaacgcct gacggctccg accattacca gcggcggtaa tcctccggca
    10501 ttttccctga cacctggcgg acggctgacg gcgaaaaatg ccgatatcag cggtaacgtg
    10561 aacgcgaact ccgggacgct caacaacgtc acgattaata agaactgtcg ggttctggga
    10621 aaattgtccg cgaaccagat tgaaggcgat ctcgttaaaa cagtgggcaa acctttccca
    10681 cgggactccc gggcaccgga gaggtggcca tcagggacca ttaccgtcag ggtttatgac
    10741 gatcagccgt ttgatcggca aattgttatt cccgcggtgg cgtttcgcgg tgctaaacat
    10801 gagcggaaga ataacaatat ttattcgtca tgccgcctga tagtgaagaa aaacggtgct
    10861 gaaatttata accgaacgac cctggataat acgctgatat atacgggtgt tattgatatg
    10921 cctgccggtc acggtcacat gacgctggag ttttctgtat cggcatggct ggtaaatggc
    10981 tggtatccca cagcaagtat cagcgatttg ctggttgttg tgatgaagaa atccacagca
    11041 ggtatcagta tcagctgaat tttataaccc agatacgggc accagaaatg gtgccttttt
    11101 tattgcagaa aagcgagagg taattatgcg taaactttat gctgccattt tgtccgcagc
    11161 catctgtctg gctgtatccg gtgcgcctgc atgggcgtct gaacatcagt ccacgctgag
    11221 cgcggggtat cttcatgcct cgacgaacgc tcccggcagt gatgatctga acgggattaa
    11281 cgtgaaatac cgttatgagt ttacggacac gctggggctg gtgacgtcat tcagctatgc
    11341 caacgctgaa gatgagcaaa aaacgcatta cagtgatacc cgctggcatg aggattccgt
    11401 gcgtaaccgc tggttcagcg tgatggcggg gctgtctgtg cgcgtgaatg aatggttcag
    11461 cgcgtatgcg atggcgggcg tggcttacag ccgtgtgtcg accttctccg gggattatct
    11521 ccgcgtaact gacaacaagg ggaaaacgca cgatgtgctg accgggagtg atgacaaccg
    11581 ccacagcaac acgtctctgg cgtggggggc tggcgtgcag tttaacccga ccgaatccgt
    11641 ggccattgac cttgcttatg aaggttccgg cagtggcgac tggcgcactg acggtttcat
    11701 cgtgggtgtc ggttataagt tctgattagc caggtaacac agtgttatga cagcccgctg
    11761 gttcaggcgg gctttttgtg gggtgaatat ggcagtaaag atttcaggtg tactgaaaga
    11821 tggggcgggt aaacctgtcg taaattgtgc gattgaactg cgggccagaa gaaccagtcc
    11881 gaccgttgtg gcacacgttg ttgccacttg cgtgacggac aataacggtg cttatgtgat
    11941 tgaggctgag ccggggtatt acgaggttgc gcttcactgt aacggctggc agccaacccg
    12001 tgtcggggat attgatgtgg caccgactga tgcaccgggg acactgaacg cgtttctgaa
    12061 tgcaccaaag gatggtgatt tacgtccgga ggtgatgaag cgctttgagg aaatggtggc
    12121 gcaggcgcag cagagtgccg gggc
//
LOCUS       DAEEUE010000111        12030 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.111, whole genome shotgun sequence.
ACCESSION   DAEEUE010000111 DAEEUE010000000
VERSION     DAEEUE010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12030
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.111"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(167..403)
                     /locus_tag="KLK84_003367"
     CDS             complement(167..403)
                     /locus_tag="KLK84_003367"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010570.1"
                     /translation="MPVCPRREKTRAGGAERGRAHAPPLSLRGRSDGGFCFFMPVICL
                     LLMLLFLLLLLLLLLLLLLLLLLLLLLFFFFLLL"
     gene            complement(429..992)
                     /locus_tag="KLK84_003368"
     CDS             complement(429..992)
                     /locus_tag="KLK84_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBE2010571.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVM
                     LAEISNRDHILEPSAGTGAILRAIRDTAPGAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWLPVQYYSRVIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            complement(1040..2401)
                     /locus_tag="KLK84_003369"
     CDS             complement(1040..2401)
                     /locus_tag="KLK84_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HBE2010572.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYISDMKITEI
                     PQK"
     gene            complement(2453..2683)
                     /locus_tag="KLK84_003370"
     CDS             complement(2453..2683)
                     /locus_tag="KLK84_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010573.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            complement(3718..3909)
                     /locus_tag="KLK84_003371"
     CDS             complement(3718..3909)
                     /locus_tag="KLK84_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010574.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(3906..4328)
                     /locus_tag="KLK84_003372"
     CDS             complement(3906..4328)
                     /locus_tag="KLK84_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HBE2010575.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRYIAQDWNLSD
                     DELETVMQRLDDAFEYGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(4375..4800)
                     /locus_tag="KLK84_003373"
     CDS             complement(4375..4800)
                     /locus_tag="KLK84_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBE2010576.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGLDFLYAEASVYALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(5216..5986)
                     /locus_tag="KLK84_003374"
     CDS             complement(5216..5986)
                     /locus_tag="KLK84_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010577.1"
                     /translation="MNETLNALIYRHASNLLLAQGWPEETDVDQRNPKYPGWISIYVR
                     LDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLAEDEIRAVLGAVRDAVCSVSYQVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSSVEMYLVSECIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARTEIRSMADALAKIRK"
     gene            complement(6031..6465)
                     /locus_tag="KLK84_003375"
     CDS             complement(6031..6465)
                     /locus_tag="KLK84_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HBE2010578.1"
                     /translation="MYGTCETLCRELAAKYPGDTPLMLVIWSPEEIQALADGMDISLS
                     DHEIRTVLARLEDIPEDQRTESGISSGAAMEIINNVRENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQAVVNQVRILLKNNTHEND"
     gene            complement(6479..6700)
                     /locus_tag="KLK84_003376"
     CDS             complement(6479..6700)
                     /locus_tag="KLK84_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010579.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            complement(6701..7384)
                     /locus_tag="KLK84_003377"
     CDS             complement(6701..7384)
                     /locus_tag="KLK84_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HBE2010580.1"
                     /translation="MSRFILGDCVRVMATFPGNAVDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGVELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            complement(7461..7772)
                     /locus_tag="KLK84_003378"
     CDS             complement(7461..7772)
                     /locus_tag="KLK84_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010581.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARGGRFYRSRLASVKVYASPSPFTDERPSSRFRGIALPSKRRRLRYSTVGLTRYRT
                     R"
     gene            complement(7769..8695)
                     /locus_tag="KLK84_003379"
     CDS             complement(7769..8695)
                     /locus_tag="KLK84_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HBE2010582.1"
                     /translation="MPNWCSNRMYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLF
                     LAGSAGLLQITENIRSEQCPGVTAAGRGAVSLENIAFTRWLTHLQNGVLLDEQNCLML
                     HELWLQSGTGQRRWEGLPDDVRETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVLP
                     EKTMPFDLLTVLPTRLDIEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQG
                     ENFIQVDFDTPWCQPESDVVAELSRRFGCTLEHWYAEQGCNFCGWQLYERGELVDVLW
                     GELEWSSPTDDDELPEVTGPAWIVDNVTHYGG"
     gene            9206..10168
                     /gene="parM/stbA"
                     /locus_tag="KLK84_003380"
     CDS             9206..10168
                     /gene="parM/stbA"
                     /locus_tag="KLK84_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HBE2010583.1"
                     /translation="MLVFIDDGSTNIKLQWQESDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKITLNGGDTFTIKDVKVMPESIPAGYEVLQEL
                     DELDSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGS
                     SYLADDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHV
                     MVIGGGAELICDAVKKHTQIRDERFFKTNNSQYDLVNGMYLIGN"
     gene            10171..10521
                     /locus_tag="KLK84_003381"
     CDS             10171..10521
                     /locus_tag="KLK84_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HBE2010584.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTA
                     GLALYRQDPRAPFLLCELLTKETTFSDIVNILRSLFPKEMADLNSSTIIQSSSQSEQK
                     SDEETKKNAMKLIN"
     gene            complement(10924..11376)
                     /locus_tag="KLK84_003382"
     CDS             complement(10924..11376)
                     /locus_tag="KLK84_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBE2010585.1"
                     /translation="MKFMECAVRDVIYGTNVRIVKPVNIYECELRDNVFVGPFVEIQK
                     GCVIGSGSRIQSHTFICENVTLGENCFIGHNVTFANDLFRSGAPDPSPDNWISITLGD
                     SVTVGSGAIILSPYICSGAVIGAGAVVVKPIENKGIYAGNPARLIRIL"
     gene            11906..>12030
                     /locus_tag="KLK84_003383"
     CDS             11906..>12030
                     /locus_tag="KLK84_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin B17"
                     /protein_id="HBE2010586.1"
                     /translation="MELKASEFGVVLSVDALKLSRQSPLGVGIGGGGGGGGGGSCG"
BASE COUNT         2872 a         3370 c         3122 g         2666 t
ORIGIN      
        1 gcatcggacc gtagcgacag cccgaaaccg cgccagcggg ttcggcggag actggcaggt
       61 gcgccagcag ctgcctgtcg gagtcgggcc tgacgaccgg atgccgccag gcagccggga
      121 gccgaaggtg cgcccggcag acaggacaac actgcagtag tcagaatcag agcagcagga
      181 aaaagaagaa cagtagcagt agcagtagca gtagcagtag cagtagcagt agcagtagca
      241 gtagcagtag caggaacaac aacatcagta acagacaaat gacaggcatg aagaaacaga
      301 agcccccatc agaacgtccg cgtaatgaaa ggggcggcgc gtgagcgcgg cccctttcag
      361 cgccaccggc gcgcgttttt tcccggcgcg ggcagacagg catttatcct cccgccggcc
      421 ctgtgtcatc aggcacgcag acgaataatc atcgtcggga catcggtata agcaaacgtg
      481 ccgcgcggca gctcctcgcg gacgtcagaa aacggtaaca gcttctcctg ctggcgtggt
      541 ccgttgagac agacggcgac cagcacgcca cccggacgca acagggaaaa ggcccgcagg
      601 atatgccgga tatcctgccc gtggctgaac ggcggattca tgataacccg gctgtaatac
      661 tgcaccggca gccattccat gaagtcgcca cactgaaccc tgacaccgtt aaaattttcc
      721 cgcagatacc ggaccagccc gctgttgatt tccaccgcat cacacatggc ccccggcgcg
      781 gtatcccgaa tcgcccgcag tatggccccg gtgccggcag agggttccag aatgtgatca
      841 cggttgctga tctccgccag catcaccagg cgatcacaga ccggggcagg agtcacaaaa
      901 agctgagatt cagcccggat ttcagcaacc ggcgcggcgt tatccagcct tgcgcgcatc
      961 agccggatac gttctcttaa ctgactgtgc atatcattat ctccgtcatc ccccggggat
     1021 tttcccgggg ggatatacct tatttctgtg ggatttccgt gattttcatg tcggagatat
     1081 acacattcac caggcggaaa ttattgtcca ttgtgcggcg gtaccggtat gccccgtggt
     1141 cttctgcttc tgccacactg cgcacggcct tacagtcccg gggcagggcg gcccactgtg
     1201 cctttgtcat ttcccggaaa ccttcccccg gatagttgac taccggcgga cgcttcgcgg
     1261 cctgactggc gacggcagcc tcttctgccg atggtgcttt gtagtccgtg atgcgatcgg
     1321 gcgtcacttt catcgtgccg ctgtacccga gaaaactgta atttggcgtt gtgactgaac
     1381 tcaccgcccc gttgcttttg ttcacacgga tgatggtcag ccactcgccc cggctgaaaa
     1441 cctgtccgcc cggctcaaaa tcctgtgtcc gggtaaccac gccgccgctt tcgtccagca
     1501 tggcacgctc atagatcagg cggttctgat agtggttaac ccagcgttgt tgatgctgaa
     1561 tctgccgttc atgacagcgg atcgcaattt cgcgggcctg ctccgtggtg atgatgtcat
     1621 catccagggc cgaccataac gatcgacttc cttcatactg gcttttttct gccgggcgcg
     1681 gatacgtatc cagcgggaaa caggcgctga tatggtcatg actgctgatg agttttgcca
     1741 tattcagatc cagcgattca gcccgccaca tcgtcagata tttctgcgac tgcgcgatgg
     1801 tcttttcagc cttgcgcaaa tcagcttcga tttttttgat acgacgccag cgaacgtccg
     1861 gacgttcttt atacttcgcg tgaagcagtg ccgaccgcgc ccgctcttcc cagtattccg
     1921 cacgttcaaa gagcatcacg gctcgtttca tgccgttttc aatacgctgc gcatcacgac
     1981 gggcgcggcg ttcgctgtga tgccccacaa gaatgggctg accgggcggg atcatcgcgg
     2041 ccagtctttc cacttcatca agtgcctgtg cggattcact ggcccgcttt ccgctgtatc
     2101 cggtaaaccg ctccgcccgt gcttcctgac gttcagcgag cgtgctgtct tcatcctcaa
     2161 tctctccggc aagtgagagg agcacgtctt cccggcccgg tgtccaggct ggcgcgacaa
     2221 acagcgcctg ttttggtgcc cagcggaaac cggcatcatg cactttttta tacgtctccg
     2281 ggtcaagacg tgacacggca tacaggcgca gtttgttgtc atccggtgag taggtcgcgc
     2341 ggtacatctg cacagggctg gcggcttccg gggcggtcat ggtgattgct gctaatgtca
     2401 taaaatgttc tccttccgtc tgttgccgcc cctcgcgggg cggctcacct catcagttaa
     2461 aagcgcgttt cagttcgagt gtgcggagca cctgcccgaa ggccgtgttt ttcatggcgg
     2521 ggatgtgtac accacgcccc cggtgaaaac tccgggcaat gtgtcgcccg aaggctttca
     2581 gcacggggtc aatttccggc gcggggatgt cttcgcgtct gaaacgggga tgacggcggg
     2641 gctttacagc cgctgcggaa ggtgaaacaa tacttactgt cataatttag cctcttatga
     2701 cgttaattaa tgaaaccgta aagactgttc gtgcagtctc tgcggtgtgg attaagcagg
     2761 tccctgacct gcttttttca tatctgcgtc tgacgttctc cggtgttttc ctccttctgt
     2821 tcctgcgttc tcattacctg ccagccggat gactgacaga acacataaat gcgtattgcc
     2881 ggagcctgaa ataaatcctg atttttaaag aacatcaccc gttgagtttc gggagccgcg
     2941 gttttgacgt ttcgcgggaa cctgctgctg tataccctgt ggtatgaact tcacccgttt
     3001 gagaaccttc gccgcaaggg gcatcactgc cacggcctga cgcacctgtc ctgggcagaa
     3061 cgcaaaattt tttaccgggt tttgggtaaa aaattttgtg ggctgttcat ttaccctgga
     3121 cagtcagtgc ggcaggttgt ggctgtatca gccccaggcg acagggactc atacgggtgt
     3181 gtggcaccgg caggcaatga gcagggaccc gccgcaacga cggagagcgc gtgcgcgaaa
     3241 cggcgttgcc ccttacatcc cggcggcctt ttgccgggat gccgggagcg caggccacgg
     3301 cgcgacaagg gtggcgcgac cggaacggtc gctgcaagcc cggggttttc acgcatcgga
     3361 ccgtagcgac agcccgaaac cgcgccagcg ggttcggcgg agactggcgg gtgcgccagc
     3421 agctgcctgt cggagtcggg cctgacgacc ggatgccgcc aggcagccgg gagccgaagg
     3481 tgcgcccggc agacagaaca acactgcagt agtcagaatc agagcagcag ataaaggaac
     3541 aacaacatca ggaacagttc cataacataa gaaagaaaca ctcctgagtc agacacatca
     3601 cagacgggaa gggtaacggg cgacgcgcca gcgtggcccg cttcccttcc ggcaggcgtc
     3661 agcctgccag gggttttcgc gcggactgac aggcggcctc caccgcctgc ccctgtatca
     3721 cctgtttctg ctaccctgta agatgtagtg accgcccagg aaaaccgcct gctgattcgc
     3781 cacaaaatcg cagatttcat caacaatctg cgcggtggtg agcggtttct gccccatttc
     3841 acgacgccgc gcattaatca cggccctgta attacgcaga atttcgcggg tttctgtgct
     3901 gatattcatg acatgtgctc cccgtccagc gcctgtaaca caacgtccat tgcttcgcgt
     3961 tcttccctga cgaatgcgtc accgtcgccc ccgttctcac tcccgacggc atacaggcgc
     4021 ttcatttcac tgcctgccag cgccatcact ttttccagca tcaccgccgg aactgtcacc
     4081 tgacggctgg ctcgcttttc ttccatcagc tcacgaacaa cgtcgtgaac aatgctgaca
     4141 tccgcaccat attcaaaggc atcgtccagc cgctgcatga cggtttccag ctcatcgtct
     4201 gacaggttcc agtcctgggc tatgtagcgc acatccccac gggtcagcac cacggcaaaa
     4261 acgcattcac tgtccggcac gtctgtatcc agtacatcgc ggatgatttc tttaacagta
     4321 cagtacatgt tctcgtctcc tttacagggg cggcattgcc gccccgtccg ttaatcagtc
     4381 cagtgccgcg taaatcgcgt tgcattcggg gtgaaattcg atgtgacgcc acaactgcgc
     4441 atcgcgcatc cggtaaagat gggtcagtcc tgcatccccg ctgtcgtggt acagccacaa
     4501 ctggcggtta atcacaaggg aagtcagaat gatgcctgcg gcatcggcgc tgaccgtacg
     4561 gtcaaaccag tttgcaccgt tcaccatgtg gaaacggtcg ccatccggca tcatgtaacc
     4621 gccaccgtcc ggcaggcgga tgaagtgcca gaatccgcct tcatattccg gcatcatttt
     4681 tttcgccagc gcgtatacag acgcttcggc atacagaaaa tccaggccaa acaggtaagg
     4741 cagaaaggag agacggtcgc actcttcaac attcagagtg acaggtttcg catattgcat
     4801 gatgttttcc tccgttgttc acatattccg cgacaccggg cggtgccgtg cctccatctt
     4861 tgccgggcct gcagggttgc aagggcgcaa gggaagccgc agcctgacgc gcacggctga
     4921 cgggagttgg agggtgagac cgcgaaatct gagcgggtga acgaccggaa aatcacggca
     4981 gacgggaagg agggaccggg tgaccgcagc ggttcacctt accttgcaac ccgcccgcag
     5041 aagcccggct acgatggagg cacggcacag cccgcacacg acgctgaggc cacataactg
     5101 aaaacccaca aggccatgcc ccgcatggcc gggtaaagcg gagcctgcgg cgacatcctt
     5161 ttgctctttc cggggaccgg ctggaaaagg cggagatttt tccccgccag tgtcatcatt
     5221 tccggatttt tgccagggca tccgccatgc tgcggatttc ggtccgggct tcccggacca
     5281 cctcccgcag aacaccacgg tcaaaccagc ggcggggcgg ttcatccggt atacgcagca
     5341 catcgcaggc cagcccactg gacaggatat gctcaataca ctcactgacc agatacattt
     5401 ccaccgacga aaaacgagcg ccccggttca ggatggcatc gagcaccacg ggcaggtttt
     5461 cgtcgtcttc atcgagccag caggggtgat cgctttcctt caccaccagg cgaaagcggc
     5521 gcgtctggcg ggtcagcaac gtctgaccgg tggtggtcag cgccgcacgg atacgccgcg
     5581 catcttctgc cacctggtaa ctgacactgc atactgcatc gcgtaccgca ccaagaacgg
     5641 ccctgatttc gtcctctgcc agccactcac cggcatacgg gaaagacacc tgcgttccgt
     5701 ctgccggaag taccggcagc gactgccact gactgccgga cagaacaagt tccgctccgg
     5761 ttccggtcag tttctgaatg gcagaggcca ggagcggcgg cagtacgccg ccgtgacggt
     5821 tgataagtaa cgtcgccagc cggggcgcat ccagccgcac gtaaatgctg atccagcccg
     5881 gatacttcgg gttacgctgg tcaacatccg tctcttccgg ccagccctgc gccagcaaca
     5941 ggttgctggc gtggcgataa atcagtgcat tcagcgtttc attcataatt catgaactcc
     6001 ctgtatcgcc gcccggaggc ggcgataaca tcagtcgttt tcgtgtgtgt tgtttttcag
     6061 caggatgcgg acctgattaa ccaccgcctg acggcgggtg acgcattccg ggacggcgag
     6121 gccatgatcc cgggcggccc attcacgttt ccacaatgcc tgttcagcgg tctgaatcag
     6181 ggacgccagc agttcagcag ggacggttac ctggcggttt tctctcacgt tattgatgat
     6241 ctccatcgcc gcaccggaag aaataccgga ttcagtccgc tggtcttccg ggatgtcctc
     6301 caggcgcgcc aggacagtcc tgatttcatg atcggacagg gaaatatcca ttccgtcagc
     6361 gagggcctga atttcttccg gggaccagat aaccagcatc agcggcgtgt ctcccggata
     6421 ctttgctgcc agctcccggc atagcgtttc gcatgttccg tacattctcg tctccttctc
     6481 aatctttgaa gcaggaatcc agttccagaa tttcggtata cgcctccatg aacaggcgat
     6541 tggcgcgata cagcgtctgg cgtgtcaggc gttcaacaag cacatcggaa tcaaagcgac
     6601 agcgatgctc catatcgttc agcctggccc gaaggtcaca catggtgttg gccacctccg
     6661 ccacgtcggc gcgaagtttt tcgtgtccgg catagttcat ttacgcagcc tccggttcaa
     6721 accagttatc attcgcggcc ccctgctgca tggcccgttg cacggcggcc agtcgttgct
     6781 gtccggcacg gtgatactgt tcaagcagct cgacaccgat ataccggcgt ccggactgga
     6841 gggcggccac acaggttgag ccgctgcctg caaacgggtc cagcacaatc gcgttcgggt
     6901 gtgtgaagct ctcaatgagc ggttgcaggc tggtaacggg cttttcagtc gggtgatgac
     6961 gattgcctga atatttccag cccagcacgt cgggcaatgg cttctgaggc agcgccggac
     7021 gtccttttgc caggatgtag gcgcattcgt ggcgatagcc cacatatgcg gccttcgatg
     7081 tgtaggtttt ggtaaacacc aggtggccaa ccacgctgaa tcccgcattt ttccaggcgg
     7141 ccataaagcg atcgacgcgg ttccagccgt agaagctgac catcagcgca tcttttttca
     7201 gcacgcggta catttcatta caggccggtt gcagccattc atcggtttta tcgccggcga
     7261 tggtgcgccc ggaacggtca cggaaaccga cgagatacgg cgggtcggtg aggatgaaat
     7321 cgacagcatt gcccggaaac gtggccataa cacgcacgca gtcacccagg ataaaacggg
     7381 acataacagc actccttctc ttacagagga caccgggagg gcacccctgt ggtgcccgtt
     7441 ccccgctgcc tgtgggctgt tcaccgcgtc cggtaacggg tcaggccgac cgtggaatac
     7501 cgcagccgtc gtctttttga cggcaaggct atgccgcgaa agcggcttga agggcgttca
     7561 tcagtaaagg gcgacgggct ggcgtacact ttcaccgacg ccagccggga gcggtaaaag
     7621 cgcccgcccc gtgcccgccg gacgcgggac actgaccaca ggcagcgttt atgccgcgcc
     7681 gcgtcagccg gcgcagtaaa aagaggagca cggcgacatc ctttgtcgga aaagaaacgg
     7741 gcggttgccc gcccgtctcc gtcatacttc atccgccata atgcgtcaca ttgtcgacta
     7801 tccacgcagg tccggtgact tccggcagct catcgtcatc tgtcggggaa gaccattcca
     7861 gttcccccca cagcacatca acaagctctc cgcgctcata caactgccag ccacagaaat
     7921 tgcagccctg ttcggcgtac cagtgctcca gcgtgcagcc gaaacggcgg cttaattctg
     7981 caacaacgtc gctttccggc tgacaccacg gcgtgtcgaa atccacctga atgaagtttt
     8041 ccccctgact gctgatattc aggtcatacc cacaaggcca cttcacgcca taccgttctg
     8101 tataccagtg atatgcagaa ggaacaccgt tcagaacacc gccgttaaag ccattcactt
     8161 caatatccag gcgggtcggc aaaaccgtca gcaggtcaaa cggcatggtt ttttccggca
     8221 gtacgttgtc acacagacgg ttccaccaca ccgatacatc ctcgttaccc cagatgtcac
     8281 accagtcacc tctttttgcg gtgaaatgca cagtgatagt ttccctgaca tcatccggta
     8341 acccttccca gcgacgctgt ccagtaccgc tctgcagcca gagttcatgc agcatcaggc
     8401 agttttgctc atccagcagt acaccgttct gcaggtgtgt cagccagcgg gtgaacgcga
     8461 tattttccag tgatacagca ccacgtccgg cagccgtcac tccggggcac tgctcagagc
     8521 ggatattttc agtgatctgc agaagtccgg cacttccggc cagaaacagc tgaatacctt
     8581 catttgtggc gcggcgataa agcggtgtga ctgcaccgct ggccagtcgt ttaatctcag
     8641 cgatctgtgc cggttcacca gaaaaataca tacgattcga gcaccagttt ggcatgggaa
     8701 ttatcctcac aggagcatcg ggagggcgcc cctgtggcgc ccgttccccg ctgcctgtgg
     8761 gctgttcacc gcgcccggta acgggtcagg ccgaccgtgg aataccgcag ccgtcgtctt
     8821 tttgacggca aggctatgcc gcgaaagcgg cttgaagggc gctcatcaga aaagggcgac
     8881 gggctggcgt acactgtcac cgacgccagc cgggagcggt aaaagcgccc gtcccgtgcc
     8941 cggccggacg cgggacactg accacaggca gcgttcctgc cgcgccgcgt cagccggcgc
     9001 agtaaaaaga ggagcccggc gacatctttt gttatccgcc aaacaaaacc caaaaacaac
     9061 ccataccaag cccaacaaaa caccaaaaca agacaaataa tcattgattg atagttaaaa
     9121 tggggtaaac ttgacaaata aacccattta aagcccaaaa cacacccaaa aaaacaccaa
     9181 aaaaacacca taaggagttt tataaatgtt ggtattcatt gatgacggtt caacaaacat
     9241 caaactacag tggcaggaaa gcgacggaac aattaaacag cacattagcc cgaacagctt
     9301 caaacgcgag tgggcagttt cttttggtga taaaaaggtc tttaactaca cactgaacgg
     9361 cgaacagtat tcatttgatc caatcagccc ggatgctgta gtcacaacca atatcgcatg
     9421 gcaatacagc gacgttaatg tcgttgcggt gcaccacgcc ttactgacca gtggtctgcc
     9481 ggtaagcgaa gtggatattg tttgcacact tcctctgaca gagtattacg acaggaataa
     9541 ccaacccaat acggaaaata ttgagcgtaa gaaagcaaac ttccggaaaa aaattacatt
     9601 aaatggtggg gatacattca caataaaaga tgtaaaagtc atgcctgaat ctataccggc
     9661 aggttatgaa gttctacaag aactggatga gttagattct ttattaatta tagatctcgg
     9721 gggcaccaca ttagatattt ctcaggtaat ggggaaatta tcggggatca gtaaaatata
     9781 cggagactca tctcttggtg tatctctggt cacatctgca gtaaaagatg ccctttctct
     9841 tgcgagaaca aaaggaagta gctatcttgc tgatgatata attattcaca gaaaagataa
     9901 taactatctg aagcaacgaa ttaatgatga gaacaaaata tcaatagtca ccgaagcaat
     9961 gaatgaagca cttcgtaaac ttgagcaacg tgtattaaat acactcaatg aattttctgg
    10021 ttatactcat gttatggtta taggtggtgg cgcagaatta atatgcgatg cagtaaaaaa
    10081 acacacacag attcgtgatg aacgtttttt taaaaccaat aactctcaat atgatttagt
    10141 taacggtatg tatctcatag gtaattaatg atggacaagc gcagaaccat tgcctttaag
    10201 ctaaatccag atgtaaatca aacagataaa attgtttgtg atacactgga cagtatcccg
    10261 caaggggaac gcagccgcct taaccgggct gcactgacgg caggtctggc cttatacaga
    10321 caagatccca gggccccttt ccttttatgt gaactgctga cgaaagaaac cacattttca
    10381 gatatcgtga atatattgag atcgctattt ccaaaagaga tggccgattt aaattcttca
    10441 acaatcattc agtcctcttc acaatcggag caaaaaagtg atgaggagac aaaaaagaat
    10501 gcgatgaagt taataaacta attaaattat gattgagttt cctttatcca ctatcaggct
    10561 ggataaaggg aacttaatca agttatttta ttgccagtca ttacataaaa taaacagggt
    10621 cgttattatg aaataatttc acggaactta tgaaaaagtg gctccaataa tcaaaattgg
    10681 tgagttcgca atgtctggca ataacgtttt tgatgggtta tttgccagtc cggatcgcca
    10741 gaatacgcat actgtcaggg ctgggaaacg atgctctcgg ttatttactg aaaaaaggtg
    10801 atatcactga cgcagaatac cgggcggccc tggaaatcga tccccggcag tcgatttctg
    10861 tacacctgcc gtattcacag acctacctct gataaaaagg gccagtaacg gcccttttct
    10921 ttttcataat attctgatca gtcttgccgg gtttcctgca taaatgcctt tgttctcaat
    10981 cggttttact acaactgccc ccgcaccaat aacagctccg ctgcatatat acggagacag
    11041 aataatggct ccactgccaa cagtaaccga atcccccagg gtaatgctga tccagttatc
    11101 cggtgacgga tctggtgctc ctgaacgaaa cagatcgttg gcgaacgtca cgttatgacc
    11161 aatgaagcag ttctccccca gtgttacgtt ctcacaaata aacgtgtgcg actgtatccg
    11221 gcttccacta ccaatgacac agcctttctg tatctcaacg aaggggccga caaacacatt
    11281 gtccctgagt tcacattcat aaatgttgac cggtttgaca atacggacat tggtcccata
    11341 aatcacatcc cggacagcac attccataaa tttcatactc acacgacctt taaatacaga
    11401 tgacacttgc cggaattata cagcccagat tcacttgcca tgtttcagaa tagattataa
    11461 gccgctcttt taccgatact cagcaacaaa tcaggtctgg atccacttca gccggaaacc
    11521 ggatctgcac ggcgggaaac gggcaaatga tatgatatgg aaggcggcac tcagttgttg
    11581 catatgatac cggcgtaagc cggtactgat tgacaggttt caccttaccc atccccggcc
    11641 ctgccagacc agtgggggct taaaggggta gtgtgacatc ctgacagcaa ctgaaagcaa
    11701 aatgtaaaca caatattaat ttttgtaaaa aaacaataac agtataaaca taaccaactg
    11761 atattattat caaatattta acaggcgtac aaatttagtt caaattatca ttgcaaaata
    11821 tagttaatta cggcaagtaa ctagtgttgg ccaacatact attcagatgt cataagcatt
    11881 aatttccctt aaaaaaggag tccttatgga attaaaagcg agtgaatttg gtgtagtttt
    11941 gtccgttgat gctcttaaat tatcacgcca gtctccatta ggtgttggca ttggtggtgg
    12001 tggcggcggc ggcggcggcg gtagctgcgg
//
LOCUS       DAEEUE010000112        11921 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.112, whole genome shotgun sequence.
ACCESSION   DAEEUE010000112 DAEEUE010000000
VERSION     DAEEUE010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11921
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.112"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..2338)
                     /locus_tag="KLK84_003384"
     CDS             complement(<1..2338)
                     /locus_tag="KLK84_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HBE2010587.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTDGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELVNV"
     gene            complement(2272..2895)
                     /locus_tag="KLK84_003385"
     CDS             complement(2272..2895)
                     /locus_tag="KLK84_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HBE2010588.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2892..4580)
                     /locus_tag="KLK84_003386"
                     /pseudo
     CDS             complement(2892..4580)
                     /locus_tag="KLK84_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4729..7356)
                     /gene="gyrA"
                     /locus_tag="KLK84_003387"
     CDS             complement(4729..7356)
                     /gene="gyrA"
                     /locus_tag="KLK84_003387"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HBE2010589.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDEKVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7503..8225
                     /locus_tag="KLK84_003388"
     CDS             7503..8225
                     /locus_tag="KLK84_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HBE2010590.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8353..10284)
                     /gene="yfaL"
                     /locus_tag="KLK84_003389"
     CDS             complement(8353..10284)
                     /gene="yfaL"
                     /locus_tag="KLK84_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HBE2010591.1"
                     /translation="MEITANVDTRSTTTEHGRDIEMRADGEVAVDAGVDTQWGALMAD
                     SSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDVADIFPYASSLWVGDG
                     ATFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATDG
                     VTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAGDTLTVNGDYTGGGTL
                     LFDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQN
                     NAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPEPDPTPDPDPTPDPDPT
                     PDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGGRYHYT
                     AAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGGYSDNQGDSRSNMTGTRADNQ
                     NHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVSEHEDGVDRYHSSGIIASLEV
                     GYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDDIQTRLGLHSEWRTAV
                     GVTPTLDLNYYHDPHATEIEEDGSTISDDAVKLRGEIKVGVTGNISQRVSLRGSVAWQ
                     KGSDDFAQTAGFLSMTVKW"
     gene            complement(10297..11892)
                     /locus_tag="KLK84_003390"
     CDS             complement(10297..11892)
                     /locus_tag="KLK84_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AidA family protein"
                     /protein_id="HBE2010592.1"
                     /translation="MTNNASGGAVFLQQGAEFSLLPENETGMTLFANNTVTGEYNNGG
                     AIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNAINNIANDGKGG
                     AIYTINNDVYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIINNTAF
                     TNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSTAAGG
                     FMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTG
                     EMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQAELNVGSTQQTF
                     VHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSYVLAGAQSMALTGD
                     IVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSGS
                     SGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASGT
                     LMVSDNSQLGDTHYNRQVIFTDK"
BASE COUNT         2758 a         3307 c         2944 g         2912 t
ORIGIN      
        1 cgacattgac cagttcacca ggctggtaat gggttttgtc cgttttcacc cggatatcaa
       61 gctgaggaac ggcaactttg atcccggcgt tctgaaaact gtactgacca ttacgggtat
      121 acagcaccga aaaagtgatg ttaggcgcaa aggaattgct cactggtacc cgggcttcat
      181 acagggtatc gtttaaacgt tgtagcgtta gccagtttgc cggatgcgaa agcagcgact
      241 gctgttccac gcgatcgcgt tccagcgtca ataacgcttc atcaattggc tccgggaaag
      301 taatcagcat cttcgcggtt tcgcctggct ggtacagcgt tttatccgcc acgatatcta
      361 ccgtaccagt atgcgccgtg ctgcccttac cgctgacggc atgacttaac ccagcgagaa
      421 ttaagccgtc tttatcgcgt aatgtcagat tgtagttgcc aggtttagcg aaattgacgg
      481 taaaggattt gccgcctgac ggtagctctc cgctatggct cgtgcggtct tcgagacgca
      541 accattcata cgtaacagga acctgttttg aagattccag cgcggcataa cggaacacaa
      601 ccgactcgcc gctattactg tattgtgcgg cggtacttaa agagtaatgc gccaggccgc
      661 gttcaatgag gatctctttg gtggtggtga cgcgatacgc cgcgccgtca ctggcggaga
      721 cggttaacaa atagcggctc ggtttatcgg cggcggggag atttaacgcc acatgaccgc
      781 tggcgtcgga caccgtttcg ctgccttcca gcgacacggg gaaacgtccg gcataacgca
      841 aatcgttacc gaccattgat aattgctgag cgcgcaaact taactgcacg cgggcatttt
      901 ttaccggctc gccatccgga tagagcagtt gcagtttgcc gctgaccgct tcgccagttt
      961 tgaactcttt tttgtcgaga gctaaaccaa tctcgaaatg tggcttgatg tagtttgcca
     1021 cgcgaaaact gctgctatag acctgattgc ggtaagcaag acgtaactca taacctccgg
     1081 caacggcatt ttctggcagg cggaaacttc cctgcccgcc attgcgcgca tccagcgtga
     1141 catcgacggt ttgcaacaga ctgccgttag cgtccagcac cgaaagcttc gccggggcgc
     1201 tgacgatggg ggatgaatgc aacggatcgc ggaactcgcg gcccatcact ttaacatcga
     1261 cacgatcgcc tgcgcgatat agcgggcgat cggtaaaaat atacaagcgg gtgttgtaga
     1321 tttcgctttc gtagaagaag ttctcggaga caaaaacgcc gccttcagca tccttaccca
     1381 gaatgtatga acgttctggc gatatatgtt gtaactgcaa agtaccgctg tcatcggtca
     1441 ccccgcgggt catcacgcca agaccgtcag tccacaagat ctctgagccg ggcttcgctt
     1501 caccctgttt tttacccgcg gtccatacca gaagctcgtt acctgacact ttgctaagcg
     1561 ccacggtatc ggaaacaaac accaccgtcg tcgcccgata cccacctacc atcgcctcga
     1621 cgaggtacag ccccggctct tgttggccga gaggaatata aatattaccc ggctgcggcg
     1681 agatgaaatt gctggatgcg ccttccagtt ttaccccttg ctgcggctcg accggtttag
     1741 cctgccatag tggataacgg aactgttcca ccaagggata ttttttcagc ggggaaaact
     1801 ggttgttctg tacataacgg gaaggtttaa taatggcatt gccgagatgt aattcgggta
     1861 atgcctgagt cacattctgc cgtgactgag aagagaaagt acgctgcatc acgcggcgag
     1921 atttgccgta ccagttatcc cacagccagg tcagcgtatt gttcagcccg tcgcccagat
     1981 attgcggttg caccacaatg cgatgcaggt ttttctgctg gcgcaaaaat gccatcgggt
     2041 caggaatacg atataggcga acgtccacgc cgccgtactc ttccatctga tagcgccgat
     2101 aatcacgccc cggcgcttcc agtcgcactt tcgcctcttc actgctgcta aaactgctgt
     2161 cagcaagcaa aaagaatgtt cccccggcgg gcggcgcata gttactggaa ggaagcgaat
     2221 catcagcttt agcaagccct gttcccacaa gagacaggga caagcacgct gctatcgcgc
     2281 cagaaaattt aaacgataaa tgccaatgaa attgggattg gaatcgttgg gtatccatcg
     2341 ggtgtccttc catgtcataa gttgttgcag actgactgcg cgcattccgt tgtcagtttt
     2401 cgtggcgctt ccggtgtggt agatgacgta acgccccatc cagaccatta agtgctgggc
     2461 atcgccctga tcgaaaaaaa tcatatcgcc aggcagcgcc tggtttatgt cttggccaat
     2521 aaactggctg ttgtactgaa tcaaattaat cgcggtcacg taggggccgt ttttcccgct
     2581 cccctgattc cagttttgcg ccagttgacg ctgttcaggt gttagcgtca tctctggcgg
     2641 caaatactgg ctagataaac cgttactttt tagccattta ctgtcgtgaa ctttcagcgt
     2701 ctcgttcgcc gcaaatcgca ccaggcccgc acaatcctgc tgataccagc gtggacttgg
     2761 cccctggcgg agctgttctt gtgcaatgcg tacaaaccag gcgcgaaaca gcctggattg
     2821 ttcgacattc agcatttcgc tatgggcaac aacacaacac agccagcaaa tcagcgccag
     2881 cagcccgtgt ctcatagcgg ctgccaggtt atgggtagcc actgccaggc gacaccgggt
     2941 tccatctggg ccagcttcat gacataacgc ggttgttgag ataaagcgtc cagcttcggc
     3001 attaataaag tttgtgcggc gttataaaaa accggttcga gattcttcgg cagactggtc
     3061 aacgtttcgt tacgcagcag tttcgccacg ccttgtggat tgatatagag cggaacgatg
     3121 ccatcagtgg gtattacatc caccattgcc gggcgggttt tattcagtgt ttgcagggcg
     3181 ttattaacca gcgtgtcatc gagggagaaa agcagcgttt tgttttgcat cgccagcgac
     3241 acacggaaaa aataatccga cattaattga tcgggttgcg ccgcctgcgc tttcggatac
     3301 tgaccgtatc gggaactcac ttcgcgacac cagatttgcg cttcgccctg ctgagtctgg
     3361 cttaccggca aaacaccttc tggcgctttg ctttcgtgcg caccaatatt ttgcgtaaac
     3421 agtttccctg gcaattgcgc ctgttcggca gtgccatcaa actgaccgac aaacagcggg
     3481 gtttgcaatt ttgagtcttc ataccagcac agccccgcgg caccgtctaa cgccccattc
     3541 agcttgtcgt tttcctggct gatgtgcgaa agcatctctt cggcaatacc gtgtgaatac
     3601 ggcaccgcca cacagaagct ggctccggca ggcatactgt tccagaccgg cgtaaaatcg
     3661 aaactggcat ctacgctggc ggattcatca tttaacgcca caaaactgtg ccagccgtcg
     3721 ttacccattt cgaagcgtac gccagcaaaa gaaggcatta accgctggta gccaaaaccc
     3781 agcaacctgg cgctgaccac gatgcgcagg cgtactggcg ttttttcggc tgtacgctct
     3841 tccaggccaa agctggcttg ccagcgtttt ttaccgctca acaaatcact tgcgatcgcc
     3901 gtggcttcgg tatcctgctg atcatcttta aacaacatat ccgtactgga aaacaccagc
     3961 atcttgtcct gatatgtcgc gaacatcagg gcgttattgc cgttatagcg caactgataa
     4021 acaggaatag tttcactatt tatcttgatg ctactgattt ccgttttgct taactagctg
     4081 tcgctggtag cggcaaacaa caatggctcc agcagtttgc ttaaaccact gcgctggatc
     4141 agcaccatgt aatgtgaaag atggccttgt ttatcgtgcc atagcgccgc ctgcgcgggc
     4201 tgatctaaga gtgacgaaaa gagcttatct ttcagcgtga gatcgtgttc atagacaatg
     4261 cggcgaatgc tgccttcaat gcccagacga tcggcatgat tctgataata gaaaacgaaa
     4321 tcttcgctca gaacatcgtg gagaaacgga atggtgagga gatctttggg aagctggctc
     4381 agagagtcgc tgtctagaaa gaggtccggc tcattgagat cgatttgcag attgttgtgc
     4441 accaccagcg gcgacaacgt tttttctggc ccactgccag catattgcaa cgcccagacg
     4501 ccagcggaaa gcagtgctat tgcgccaaaa cctacaagac catagaaccg ccagcctttc
     4561 gccttttttt caccactcat tgccacattc cttgtgtata gccagccatt ttttacgggc
     4621 acagccaaac tttaccgcgc cctaatacga caaaagccca gactttgcag cctggacttt
     4681 tcaattcaaa caagggagat agctcccttt tggcatgaag aagtaaaatt attcttcttc
     4741 tggctcgtcg tcaacgtcca cttccggagc aatttcatca tccccttccg cggcactgcc
     4801 gtcgatggta tccagatctt cctcgtcaac cggttcagca acacgctgca gacccactac
     4861 tttttcatct tccgcagtac ggatgaggat cacgccctgg gtgttacggc ccacgatgct
     4921 gatttctgaa acgcgagtac gtaccagcgt accggcatcg gtgatcatca tgatctggtc
     4981 gcagtcatct acctgcaccg cgccaaccac taaaccgtta cgttcggtaa ccttgatgga
     5041 gataacccct ttcgtcgcac gcgacttggt tgggtattcc gccactgcag tacgtttacc
     5101 gtaaccgttt tgcgttgcgg tgaggattgc gccatcgcca cgaggcacga tcagagagac
     5161 gactttatcg ccttcaccta agcgaatacc gcgcacacca gtggtattgc agcccatcgc
     5221 acggacagaa gactctttaa agcgcaccac tttaccttcg gcggagaaca gcattacttc
     5281 atcttcgcca ctggtcagat caacgccgat cagctcatcg ccgtcaacca gtttgatcgc
     5341 cactttaccg gcggtacgca gacggttgaa ttcggtgagg acggttttct tcacagtacc
     5401 gttagcggtc gccatgaaga ctttcacgcc ttcttcaaac tcggtcaccg gcaggatcgc
     5461 ggtgatacgt tcgtcctgtt ccagcggtag caggttgacg atcggacgac cgcgcgcgcc
     5521 acgagtggct tccggcaact ggtaaacttt catcgaatag acgcgaccac ggctggagaa
     5581 gcacagaata tggtcgtgcg tgttcgccac cagcagtcgg tcgataaagt cttcttcttt
     5641 aatacgtgcg gcagatttac ctttcccgcc acgacgctgc gcttcgtatt cagaaagcgg
     5701 ctgatattta acgtagccct ggtgagagag cgtcacgacc acatcttcct gggtgatcag
     5761 atcttccagg ttgatgtctg cgctgttggc ggtgatttca gtacgacgtt tgtcaccgaa
     5821 ctgttcacga accagctcca gctcttcgcg gatcacttcc atcagacgat cggcgctacc
     5881 aagaatacgc aacagttccg cgatctgatc cagcagctct ttgtattcgt cgagcagttt
     5941 ttcgtgctca aggccggtca gtttctgcaa acgcagatcc agaatcgcct gagcttgctg
     6001 ttcggtcagg tagtacagac catcacgcac gccgaactca ggctccagcc attccggacg
     6061 cgcagcatcg tcgccagcac gttccaacat ggcggcaacg ttgcccagct gccacggatt
     6121 agcaaccagc gcagttttcg cttctgcagg cgtcggcgca tgacggatca gttcgatgat
     6181 cgggtcaatg ttcgccagcg ccacggctaa tgcttcaagg atatgagcac gatcgcgagc
     6241 tttacgcagt tcgaaaatag tacgacgggt caccacttca cggcggtgac gaacaaacgc
     6301 cgcgatgatg tctttcaggt tcatgatctt cggctgacca tggtgcaatg ccaccatgtt
     6361 gataccgaaa gaaacctgca actgggtctg ggagtagagg ttgttgagca caacttcacc
     6421 gaccgcatcg cgtttcactt caatcacgat gcgcataccg tctttgtcag actcgtcacg
     6481 cagcgcgctg atgccttcca cgcgtttttc ttttaccagt tccgcaatct tctcgatcag
     6541 gcgcgctttg tttacctgat acggaatttc gtggacgata atggtttcac gtccggtttt
     6601 ggcgtcaact tccacttcag cacgggcgcg gatatatacc ttgccgcgac cggtacggta
     6661 agcttcttca ataccgcgac gaccgttaat gattgccgcc gtcgggaagt ccggccccgg
     6721 gatgtgttcc atcagccctt caatgctgat gtcttcatca tcgatatacg ccagacaacc
     6781 gttgatgact tccgtcaggt tgtgcggcgg gatgttggtt gccataccta cggcgatacc
     6841 ggaagaaccg ttcaccagca ggttaggaat tttggttggc atgacgtcgg gaattttttc
     6901 cgtgccgtca tagttatcaa cgaaatcgac cgtctctttt tcgagatcgg ccatcagttc
     6961 atgggcaatt ttcgccagac ggatttccgt ataacgcatt gccgccgcag agtcgccgtc
     7021 gatggaaccg aagttaccct gaccgtctac cagcatgtaa cgcagcgaga atggctgcgc
     7081 catacggacg atcgtgtcat aaaccgccga gtcaccatgg ggatggtatt taccgattac
     7141 gtcaccaacg acacgggcag attttttata ggctttgttc cagtcattgc ctagtacgtt
     7201 catggcgtaa agtacgcgac ggtgtaccgg cttcaggcca tctcggacat ctggcagcgc
     7261 acggccaaca atgaccgaca tcgcataatc cagataggag ctctttagct cttcctcaat
     7321 gttgaccggt gtaatttctc tcgcaaggtc gctcatctaa ccgctatccc tctactgtat
     7381 cccggattca aaggtcgcaa attataacac agccgcgcag tttgaggtaa acctatacgc
     7441 tttattcaca tccaatgcct gatatactcg tttgtcttgc caattacgga gtagaagtgc
     7501 caatgaatgc cgaaaaatcg ccggaaaacc ataacgtaga ccacgaagag atcgctaaat
     7561 ttgaagccgt cgcctcccgc tggtgggatc ttgaaggtga gttcaaaccg ttgcaccgca
     7621 ttaacccgct gcgtctgggc tatattgccg agcgtgctgg cggtttattt ggcaaaaagg
     7681 tgctcgatgt cggttgtggc ggcggcattc tggccgagag tatggcgcgc gaaggcgcga
     7741 cggtgaccgg tctggatatg ggctttgagc cattgcaggt ggcaaagctg cacgcgctgg
     7801 aaagcggcat tcaggtggat tacgtgcagg aaaccgtgga agagcacgcg gcaaaacatg
     7861 ccgggcagta tgatgtggtg acctgcatgg agatgctgga gcacgtaccc gatccgcagt
     7921 cagtggtcag agcctgtgcg caactggtga aaccaggcgg cgatgtcttt ttctcgacgc
     7981 ttaaccgcaa cggcaagtca tggctgatgg cggtggttgg cgcggaatat attttgcgca
     8041 tggtgcccaa aggtacgcat gatgtgaaga agtttattaa accggcagag ctgcttggct
     8101 gggtggatca gaccagttta aaagagcgac atataaccgg gctacattac aacccgatca
     8161 ctaattcctt taagctcggc cccggcgtgg atgtgaacta tatgctgcac acgcagaata
     8221 agtgaggttg atgtttggcc gcgccaatgc ctgatgcgac gcttgcagcg tcttatcagg
     8281 cctacaaatg ctctccgtag gccagataag gcgtttacgc cgcatccggc aattgtgatg
     8341 gtcagacagc gattaccatt tcaccgtcat cgacaaaaac cctgccgtct gggcaaaatc
     8401 atcactccct ttctgccacg ccacgctgcc gcgcagggac actcgctgac tgatattgcc
     8461 cgtgactccc acttttattt caccccgtag cttcaccgca tcgtcactga tagtgctgcc
     8521 atcttcttca atttccgtcg catggggatc gtgatagtaa ttcagatcta atgttggtgt
     8581 gacaccaacc gcggtacgcc attcgctgtg taaacccagc cgcgtctgaa tatcatcccc
     8641 ctgcgattgt gacacgcgcg catggttagc ggcggtaaaa tcatcctgct gcacgccctg
     8701 ataaatcacc tgcgcctgcg gttcaatcac cacaccacgc cccggtaacc actgataccc
     8761 gacttccagc gaggcgataa tccccgacga atggtaacga tccacgccat cttcatgttc
     8821 ggaaacatca ttgttaaacc acgcgtattg cagccagcta tccagccagg ccccctgctt
     8881 ctgattaccg tgctgatacc agcttgaggt cagcccaacg gcataaccgt ggttctggtt
     8941 atcggcgcga gttccggtca tattcgagcg gctgtcgccc tgattatcac tgtagccacc
     9001 aaccgcccca agcatccact cgccatccgt gccccagcgc ccgctaaaca agccgccgct
     9061 aagctgcacc gtagaagtgt cttcctgctg agccagttgc cctgccgccg tgtaatgata
     9121 acgtccgccg ataacacgta aattcagcgt ctgaccatcg ccaccagcgt gatcgcgtcg
     9181 ctccatcata aacgcctgat ttgccgcccg caggttattg agataaccgc caactttggc
     9241 attcagcacc ggctggtaag caggcgtagg ggccgggtcg ggggttgggt caggatccgg
     9301 cgtgggatca ggatcgggag tcgggtctgg ctcaggtggc gatggcggcg ttacttcttg
     9361 cgatcgcaga taccagtcgt tgttatcttc cactagcgtg tagtcatacg ctcccatgtt
     9421 gacgtagcca ctgccagcca gactgaactg tgcattgttt tgaaactgcg tgggatcagc
     9481 tgcgaaatca accactttaa tgcctgtcga tgtcggctca ccaatccctg taatggagtt
     9541 aaccaccaca gttgtgttgc cagcagtatt accgttcatc accaattgat cgcttaccga
     9601 gtcatcgccg tttaattcgc tgtcgaatag tagcgtaccg ccgccggtat aatcaccgtt
     9661 taccgtcagc gtatcgccag cgacgccatt ttgcaggcta accgcaccgg acgtattggt
     9721 cagatcacca ttaaccgtca cgtcagcaag ataggtcagg ctgtcagttt caaggttggt
     9781 attaagctcg cctgtcaacg tcacgccatc ggtagcattt accagcgtcc catcgccgtt
     9841 aaatagtgag gcattaaggg cgacggaagt atcctgcccg gtcagacgca aaaccgtacc
     9901 atcgctgatg tcgatagtgc cgctggaagt agcatcaatt gactgaatat cctgatccgc
     9961 gccagtaacg aacgttgccc catcaccaac ccacagtgac gaagcataag gaaagatatc
    10021 cgcaacatcg cctttcaggg tgccttcttc gacacgtacc gccgactgcg ttgtaccgct
    10081 ggcggtcagc tccagcgtac ccgcccccgt tttagtaaat gtgctaccct catcctgatg
    10141 ctgcccgctg ctgtcagcca tcagtgcgcc ccactgcgtg tctacccccg catcaactgc
    10201 cacttcaccg tcggcgcgca tttcaatatc acgcccatgc tcagtcgtcg tagagcgagt
    10261 atcgacattg gcagtaatct ccatcacgct ttcttgttac ttatcggtaa agataacctg
    10321 gcggttatag tgggtatctc caagctgcga gttgtcgctc accatcagcg taccggaggc
    10381 gatttgtgtt gtgcccaaat aactattatt gtttgccaga cttacctgcc cgtcgctcgc
    10441 atcgaccacc acgtagacgc catctttccc gtcgccgccg atatgcaaat tgtcgccacc
    10501 tgcaagatct accacatcct gactgccgat aaccgttccg ctgctgccac tgacttcaag
    10561 gccatcattg tatgatgtgc cgctctgcca agtggagaaa tcagagagat cgagcacacc
    10621 gccgtttaac acgatcgact gcggatcgtc ctggagagcg gtaagatctg ccgcgtcgcc
    10681 ttccagcgaa agcaccgcac catcatcaac gactatatcg ccggttagcg ccatcgactg
    10741 cgcccctgcc agcacgtagc tgccgttttg cgcaatggtg agctgaccag caccttcgat
    10801 gatgccagca aaactgccct gattaacagt aacgttgcca ccagcatcga tatttaaagt
    10861 gccattctga aagcccgtca atgcgtgcac aaaagtttgt tgggtcgagc caacgtttag
    10921 ctcagcctga ttctgatact gatcaatact ccctatcgtc agaccgtagc agtcttgcgg
    10981 atcgtcctgg caatgcgtat cgccgacatt catcagggag ttgctgcgac ccagggtaac
    11041 ttcaccgttt tcaatctgca tctcaccagt aaagtcattg ttatctgcat taagtaccag
    11101 atcgccggaa cctgttttgg tgattaaccc ggtaccagca atagagtcaa cagctccgtc
    11161 attctctgta ttgccgataa ccagcgtttt tccgtcggca atatcaaagg taacttcact
    11221 taagccgaga tacataaagc cacccgccgc agtggaggga ccatctccat agcctgctgc
    11281 gctattgttc tcatcgacta acacgccgcc gttctggctg tagctgtcat caacagaaat
    11341 atcaataaga tagggagccg tcgcgctatt ggtatatatc gccccgccat aaccttcggc
    11401 tgtgttattt gtaaaggcgg tgttatttat tatcgtataa cctgaaggat gcttgctgtc
    11461 gctattatta tcggtaacat cgattgcccc gccatcgcca tcactgtaac ttgttgatgt
    11521 atatgcctgg ttattgttaa aaacatcatc acttaaatag acgtcattat taatggtata
    11581 aattgcgcca cctttgccat cattagcgat attattgatg gcgttagtga cacgtaaatc
    11641 gacggcacca gtatcattag taccagaaga atagattgcg ccaccatagc cgcctgcgac
    11701 gttaccggaa aaaataacat cagtaagatt cagcgttgag ttttctttag caaatatggc
    11761 cccgccgtta ttatattctc ctgtaacggt gttattggca aacagagtca ttccagtttc
    11821 attttctggt aatagtgaaa attccgctcc ttgttgcaaa aatacggccc caccgctggc
    11881 attattggtc atatccgaaa aaaccagcca ttgcccatcg g
//
LOCUS       DAEEUE010000113        11666 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.113, whole genome shotgun sequence.
ACCESSION   DAEEUE010000113 DAEEUE010000000
VERSION     DAEEUE010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11666
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.113"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            597..794
                     /locus_tag="KLK84_003391"
     CDS             597..794
                     /locus_tag="KLK84_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010593.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="KLK84_003392"
     CDS             complement(744..1937)
                     /locus_tag="KLK84_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE2010594.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="KLK84_003393"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="KLK84_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HBE2010595.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="KLK84_003394"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="KLK84_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HBE2010596.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="KLK84_003395"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="KLK84_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HBE2010597.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="KLK84_003396"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="KLK84_003396"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HBE2010598.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="KLK84_003397"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="KLK84_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HBE2010599.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10573..10716)
                     /locus_tag="KLK84_003398"
     CDS             complement(10573..10716)
                     /locus_tag="KLK84_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010600.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10966..>11666
                     /locus_tag="KLK84_003399"
     CDS             10966..>11666
                     /locus_tag="KLK84_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBE2010601.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIRGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQW"
BASE COUNT         2961 a         2898 c         2993 g         2814 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgctcagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctgccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc
    10501 cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg
    10561 ctataggcaa agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt
    10621 ctgaataacg cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat
    10681 cgctaattcg tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg
    10741 aaatattaaa cattacccct ccccacattg ggcaaaaaaa aacaaaaaac gatcaattaa
    10801 cgatcaatta gtattgattc acataatctc tcatcattaa tgagaacatt gtcacatatt
    10861 tacaatatag aactgttact tttagcaagc tgttagtaac caaccgttac taataatttt
    10921 aattcttaat actttgttca ttcaataaat gagttgagag aatatatgaa taaaatatac
    10981 tcccttaaat atagtgctgc cactggcgga ctcattgctg tttctgaatt agcgaaaaga
    11041 gtttctggta aaacaaaccg aaaacttgta gcaacaatgt tgtctctggc tgttgcaggt
    11101 acagtaaatg cagcaaatat tgatatatca aatgtatggg cgagagacta tcttgatctt
    11161 gcacaaaata aaggtatttt ccagcccgga gcaacagacg taacaatcac tttaaaaaac
    11221 ggagataaat tctctttcca taatctctca attccggatt tttctggtgc agcagcgagt
    11281 ggcgcagcta ccgcaataag aggttcttat agtgttactg ttgcacataa caaaaagaac
    11341 cctcaggccg cagaaactca ggtttacgct cagtcttctt acaaggttgt tgacagaaga
    11401 aattccaatg attttgagat tcagaggtta aataaatttg ttgtggaaac agtaggtgcc
    11461 accccggcag agaccaaccc tacaacatat tctgatgcat tagaacgcta cggtatagtc
    11521 acttctgacg gttcaaaaaa aatcataggt tttcgtgctg gctctggagg aacatcattt
    11581 attaatggtg aatccaaaat ctcaacaaat tcagcatata gccatgacct gttaagtgct
    11641 agtctatttg aggtcaccca atggga
//
LOCUS       DAEEUE010000114        11601 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.114, whole genome shotgun sequence.
ACCESSION   DAEEUE010000114 DAEEUE010000000
VERSION     DAEEUE010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11601
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.114"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(282..357)
                     /locus_tag="KLK84_003400"
     tRNA            complement(282..357)
                     /locus_tag="KLK84_003400"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(322..324),aa:Ile,seq:cat)
     gene            916..3138
                     /locus_tag="KLK84_003401"
                     /pseudo
     CDS             916..3138
                     /locus_tag="KLK84_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3474..4451
                     /gene="csiD"
                     /locus_tag="KLK84_003402"
     CDS             3474..4451
                     /gene="csiD"
                     /locus_tag="KLK84_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HBE2010602.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4471..5739
                     /gene="lhgO"
                     /locus_tag="KLK84_003403"
     CDS             4471..5739
                     /gene="lhgO"
                     /locus_tag="KLK84_003403"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HBE2010603.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5762..7210
                     /gene="gabD"
                     /locus_tag="KLK84_003404"
                     /pseudo
     CDS             5762..7210
                     /gene="gabD"
                     /locus_tag="KLK84_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            7218..8504
                     /gene="gabT"
                     /locus_tag="KLK84_003405"
     CDS             7218..8504
                     /gene="gabT"
                     /locus_tag="KLK84_003405"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HBE2010604.1"
                     /translation="MRRNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGRE
                     YLDFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGD
                     FAKKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGEYHGRTHYTLALTGKVNPYSAG
                     MGLMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIMIEPVQGEGGFYA
                     ASPAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGF
                     PLAGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLK
                     DGLLAIAEKHPEIGDVRGLGSMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSC
                     GPYYNVLRILVPLTIEDAQIRQGLEIIGQCFDEAKQ"
     gene            8742..10142
                     /gene="gabP"
                     /locus_tag="KLK84_003406"
     CDS             8742..10142
                     /gene="gabP"
                     /locus_tag="KLK84_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HBE2010605.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVISFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10163..10825
                     /gene="csiR"
                     /locus_tag="KLK84_003407"
     CDS             10163..10825
                     /gene="csiR"
                     /locus_tag="KLK84_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HBE2010606.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10826..11275)
                     /gene="kbp"
                     /locus_tag="KLK84_003408"
     CDS             complement(10826..11275)
                     /gene="kbp"
                     /locus_tag="KLK84_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HBE2010607.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(11359..11517)
                     /locus_tag="KLK84_003409"
     CDS             complement(11359..11517)
                     /locus_tag="KLK84_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HBE2010608.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2984 a         2848 c         2894 g         2875 t
ORIGIN      
        1 tccggaacaa tttgaaaacc agaacctcgc ttaggcctgt gtccatatta cgtgggtagg
       61 atcaatttgc ccgcttggca tagcgggcat tttttccagg tacttttgaa tgagtactga
      121 tgaataaata cattgcagtg gcgtgccacg taccaaaaca ccagccctca ttcgaaacca
      181 cccaccgcac ttcttccttg aaatggcgtt agtcatgaaa tatagaccgc catcgagtac
      241 cccttgtacc cttaactctt cctgatacat gaatgatgat ttggtggccc ttgctggact
      301 tgaaccagcg accaagcgat tatgagtcgc ctgctctaac cactgagcta aagggcctta
      361 agtatgcaat aacaatactt ataaaccacg caataaacat gatgatctta tgatgtaaat
      421 aacagatttt tatgcgttcc cattcacctt gggtcgtgat tacacgcgac ataaaacccg
      481 acaccgcctc cattcgcaaa gtcgatactc gcagtcaaca agcaaatgtt aataattagc
      541 actatctata gttatcatcg attcaatgat actttgtaat gattttgtat ctaataatat
      601 aactttatta cattagctga agagttttcg catcattatg atatctgtta cttttcactc
      661 cataaaaata aacttcatat agcaatatat tctttcatag atcttattct gctaatcatt
      721 agtttcgtat gcgcgatttt tgacagttgc ctctccagac cacatcgata attaaaaaac
      781 agatttaaac attatccttt tccatataga tattggataa aaataggaca tctgtttgca
      841 attactttca caacaattaa acatttttat gttttcgtat acatcatatt actctaccat
      901 tagaggaacg ttattatgtt ttctataaaa ccaggacctg gaaatttacc tatcgacaac
      961 cccacattgt tatcatggaa cattactgat ggggatctaa attccaaatt taatacatta
     1021 gaatatctaa actgtataac aaatattatt aatgcttgtg gagtttatcc tcaggattta
     1081 aaagacagag aaattatatc aacttttcac gcagaaaaag tcattaatga tctgttaaaa
     1141 aacgattata aaatttccct ttctccagat acaacttacc gagagctgaa taaagctgca
     1201 cagagtagca ttacagcgcc agacaggata ggagaaggga aaacatgggt ttatcaacga
     1261 gatacaatgg ttgaaagagg tgataacagt ggtgttcatc agtatggtcc agctgaacat
     1321 ttcactcaca ttatatctga caaaccgtcc ccaaaagata agtatgttgc atatgctatt
     1381 aacattcctg actatgagct agcagccgat gtatataata ttaacgtgac gtcaccttcc
     1441 ggacagcaag aaacatttaa aatactaatc aatccagaac atctacggca aacacttgag
     1501 cgtaaatctc ttactgctgt tcagaaatca caatgtgaaa tcatcacccc caaaaaacct
     1561 ggcgaagcga ttcttcatgc ttttaatgcc acctaccagc aaatcaggga aaatatgtct
     1621 gagtttgcac gttcccatta tgggtatata caaatccctc cagtgacaac tttccgcgcc
     1681 gacggaccag aaactcccga agaagaaaaa ggttactggt ttcacgctta tcaacccgaa
     1741 gatctttgta ccatccataa tccaatgggg gatttgcagg attttatcgc attggttaaa
     1801 gatgctaaaa aatttggtat cgatatcatt cctgattata cctttaactt tatgggaatc
     1861 ggggggagtg gtaaaaatga cctggattat ccctctgctg atatacgagc gaagatcagt
     1921 aaagatatag aaagtggtat ccctggctat tggcaaggtc aggttttgat tccattcatt
     1981 aaagatccag taacaaaaga acgtaaacaa atccatccag aagatataca tctcactgca
     2041 aaagacttcg aagcaagtaa agataacatc tctaaggatg aatggaaaaa cctccatgca
     2101 ttaaaagaaa agagtttaaa tggaatgcct aaaacaaccc ccaaaagtga ccaggttatt
     2161 atgttgcaaa atcaatacgt tcgtgaaatg cgaaaatatg gcgtacgagg tttacgttat
     2221 gatgcagcaa aacactcaaa acatgaacaa atagaaagat caataacccc accgcttaaa
     2281 aattataatg agcgattaca caatactaac ttatttaacc caaaatatca tgaaaaaacc
     2341 gttatgaatt acatggaata tctggtaact tgtcagttga atgaagaaca aatgtcatcg
     2401 ctgctttatg aaagagatga tttaagcgcc attgattttt cattgctcat gaagacgata
     2461 aaagcctttt cattcggggg agatctccta acccttgcat caaaaccggg ttccacaatc
     2521 tcaagcatcc catcagaaag acggatattg attaacatta accacgattt tcctaacaat
     2581 ggcaatcttt tcaatgactt tctatttaac catcaacaag atgaacaatt agcaatggca
     2641 tatatggccg ctctcccgtt cagcaggcct ttagtttact gggatggcca ggtattaaaa
     2701 tcaacgactg aaattaaaaa ttatgatggg tccacgcgtg tcggtggtga ggcgtagctc
     2761 tacaatgaat tccacgcatt atatatagat aaagcaggaa tatggagcgc atttgagggt
     2821 gtatttgcaa ctaaaaacgt tctggccttt agtcgtgggg attctgtgaa cattaatcac
     2881 tctcctcatg atggactagt tataataaat aaaggaaacg aagaagttga aggtacctgg
     2941 cctaacaaat tgcaacctgg aatatataaa aacatgggga gtaatagcgt taacattatt
     3001 attaataata cccgaaaaat tatcccccct tgtaaagtat ttacgcttag aggcggatct
     3061 ctaaatatca atattcctag gcgtagcgct cttcttttag ggaaaacagg agaaccgccg
     3121 aactatctct atttataatt taatgttata tctgccccga taaaacgggg cagataatat
     3181 gtttagttta ctaacggtca ttttgtagtg aagccattta ctgtttttta tcgaccagat
     3241 aatctgttct ctaatgttaa ctccccctaa cctgttgctt tagttattca tttcctgtct
     3301 cactttgcct taatacccta cgttaaatgt tactaatttg ttgcttttga tcacaataag
     3361 aaagcaatat gtcgcttttg tgcgcatttt tcagaaatgt agatattttt agattatggc
     3421 tacgaaatga gcatcgccat gtcaccctac atctcataag aggatcgctt ctgatgaatg
     3481 cactgaccgc cgtacaaaat aacgctgtcg attcaggcca ggactatagc ggattcactc
     3541 tcatcccgtc ggcgcaatcc ccgcgtctgc tggaactcac cttcaccgaa cagacgacca
     3601 aacagtttct cgaacaggtt gccgaatggc ccgtgcaggc gctggagtac aaatcgtttc
     3661 tgcgttttcg ggtaggcaaa attcttgacg atctgtgtgc gaatcagctg caaccattgc
     3721 tgttgaagac cctgctaaac cgtgctgaag gtgcgctgtt gatcaatgcg gtaggtatcg
     3781 atgatgtggc gcaggcagat gagatggtga agctggcaac ggcggtagcg catctgattg
     3841 gtcgctcaaa tttcgatgcg atgagcggtc agtattacgc gcgtttcgtg gtgaaaaatg
     3901 tcgataactc agacagctat ctgcgtcagc cgcaccgcgt gatggaactg cacaacgacg
     3961 gcacgtatgt cgaagagatc accgattacg tgctgatgat gaaaatcgat gagcaaaaca
     4021 tgcagggtgg aaattcgttg ctgctgcatc tcgatgactg ggaacatctg gaccactatt
     4081 tccgccatcc actggcgcgt cgcccgatgc gctttgccgc gccgccgagc aaaaacgtca
     4141 gcaaagatgt tttccatcca gtgttcgacg ttgatcaaca gggtcgcccg gtgatgcgct
     4201 atatcgacca gttcgtccag ccaaaagatt ttgaagaagg cgtgtggttg agcgagcttt
     4261 ccgatgccat tgaaaccagc aaaggcattc tttctgtacc cgttcccgtt ggcaaattcc
     4321 tgttgataaa caacctgttc tggctgcacg gtcgcgaccg ctttactccg cacccggatc
     4381 tgcgccgtga actgatgcgt cagcgtggct atttcgctta cgccactcac cactaccaga
     4441 cgcatcagta agcgcaaagg aattgagcgg atgtatgatt ttgtgattat tggcggcggc
     4501 atcatcggca tgtcgaccgc catgcaactg attgatgttt atccggatgc ccgcattgcg
     4561 ttgctggaaa aagagtccgg cccggcctgt caccagacgg gccacaacag cggcgtgatc
     4621 catgccgggg tctattacac gcccggcagc ctgaaggcgc agttttgcct ggcggggaac
     4681 cgtgccacca aagccttttg cgatcaaaac ggcattcgct atgacaactg cggcaagatg
     4741 ctggtcgcca cgtctcagct cgaaatggaa cggatgcgcg ccttatggga acgcacagcg
     4801 gcgaacggta tcgagcgcga gtggataaac gccgtggaac tgcgcgagcg cgaaccgaat
     4861 atcaccgggc ttggtggcat ttttgtgccg tccagcggca ttgtcagcta ccgcgaagtg
     4921 acggcggcaa tggcaaaaat cttccaggcc agaggcggcg agattattta taacgccgaa
     4981 gtcagcgccc tcagtgagca taaaaacggc gtggtgatac gcacccgtca gggcggtgaa
     5041 tatgaagcat cgacgctgat tagctgttcc gggctgatgg ctgaccggct ggtgaaaatg
     5101 ctcggcctcg aaccgggctt tattatctgc ccgttccgtg gcgagtattt ccgccttgcg
     5161 ccggagcata accagattgt taaccacctg atttacccca ttcccgaccc cgcgatgcca
     5221 tttttgggcg tgcatctcac ccgcatgatc gacggcagcg tgaccgtcgg gccaaacgcg
     5281 gtgctggctt tcaaacgcga aggctatcgc aagcgcgact tctcatttag tgacacgctg
     5341 gagattttga gctcgtcggg gattcgccgg gtgctgcaaa accatctacg ctcaggactg
     5401 ggcgagatga aaaactcgct gtgcaaaagc ggctatctgc ggctggtgca aaagtattgt
     5461 ccccggcttt cgttaagcga tctccagccc tggcccgccg gtgtgcgggc gcaggcagta
     5521 tcgccggacg gcaagctgat tgacgatttt ctgtttgtca ccaccccacg cacgatccac
     5581 acctgcaatg cgccctcccc ggcagcgaca tcagcaattc ctattggtgc gcatattgtc
     5641 agcaaggtac aaacgctgtt ggcaagccag agtaaccccg gacgcacgct gcgagcggca
     5701 cgtagtgtgg atgccttaca cgccgcattt aatcaataac ctttgaaaac aggacgtaac
     5761 aatgaaactt aacgacagta acttattccg ccagcaggcg ttgattaacg gggaatggct
     5821 ggacgccaac aatggtgaag tcatcgacgt caccaatccg gcgaacggcg acaagctggg
     5881 tagcgtgccg aaaatgggcg cagatgaaac ccgcgccgct atcgacgccg ccaaccgcgc
     5941 cctgcccgcc tggcgtgcgc tcaccgccaa agaacgcgcc accattctgc gcaactggtt
     6001 caatttgatg atgcagcatc aggacgattt agcgcgcctg atgaccctcg aacagggtaa
     6061 accgctggct gaagcgaaag gcgaaatcag ctacgccgcc tccttcattg agtggtttgc
     6121 tgaagaaggc aaacgtattt atggctacac cattcctggt catcaggccg ataagcgcct
     6181 gattgttatc aagcagccga ttggcgtcac cgccgccatc acgccgtgga actttccggc
     6241 ggcgatgatt acccgtaaag ccggtccggc gctggcggca ggctgcacca tggtgctgaa
     6301 gcccgccagt cagacgccgt tctctgcgct ggcgctggcg gaactggcga ttcgcgcggg
     6361 cattccggct ggcgtgttta acgtggtcac cggttcggca ggcgcggtcg gtaatgaact
     6421 gaccagcaac ccgctggtac gcaaactgtc gtttaccggt tagaccgaga ttggccgcca
     6481 gttaatggaa cagtgcgcga aagacatcaa aaaagtgtcg ctggagctgg gcggcaacgc
     6541 gccgtttatc gtctttgacg atgccgatct cgataaagcc gtggaaggcg cgctggcctc
     6601 gaaattccgc aacgccgggc aaacctgcgt ctgcgccaac cgtttatacg tgcaggacgg
     6661 cgtgtatgac cgctttgccg aaaaattaca gcaggcggtg agcaaactgc acatcggcga
     6721 cgggctggag aaaggcgtca ccatcgggcc gctgatcgat gaaaaagcgg tagcaaaagt
     6781 ggaagagcat attgccgatg cgctggagaa aggtgcgcgt gtggtttgcg gcggtaaagc
     6841 gcacgaacgc ggcggcaact tcttccagcc aaccattctg gtggacgttc cggccaacgc
     6901 caaagtgtcg aaagaagaga cgttcggccc cctcgcccca ctgttccgtt ttaaagatga
     6961 agccgatgtg attgcacaag ccaatgacac cgagtttggt cttgccgcct atttctacgc
     7021 ccgtgattta agccgcgtct tccgcgtcgg cgaggcgctg gagtacggca tcgtcggcat
     7081 caataccggc attatttcca atgaagtggc cccattcggc ggcatcaaag cctcgggtct
     7141 gggtcgtgaa ggttcgaagt atggcatcga agattactta gaaatcaaat atatgtgcat
     7201 cggtctttaa ctggagaatg cgaaggaaca gcaataaaga gttaatgcag cgccgcagtc
     7261 aggcgattcc ccgtggcgtt gggcaaattc acccgatttt cgctgaccgc gcggaaaact
     7321 gccgggtgtg ggacgttgaa ggccgtgagt atcttgattt cgcgggcggg attgcggtgc
     7381 tcaataccgg gcacctgcat ccgaaggtgg tggccgcggt ggaagcgcag ttgaaaaaac
     7441 tgtcgcacac ctgcttccag gtgctggctt acgagccgta tctggagctg tgcgagatta
     7501 tgaatcagaa ggtgccgggc gatttcgcca agaaaacgct gctggttacg accggttccg
     7561 aagcggtgga aaacgcggta aaaatcgccc gcgccgccac caaacgtagc ggcaccatcg
     7621 cttttagcgg cgagtatcac gggcgcacgc attacaccct ggcgctgacc ggcaaggtga
     7681 atccgtactc tgcgggcatg gggctgatgc cgggtcatgt ttatcgcgcg ctttatcctt
     7741 gcccgctgca cggcataagc gaggatgacg ctatcgccag catccaccgg atcttcaaaa
     7801 atgatgccgc gccggaagat atcgccgcca tcatgattga gccggttcag ggcgaaggcg
     7861 gtttctacgc cgcgtcgcca gcctttatgc agcgtttacg cgctctgtgt gacgagcacg
     7921 ggatcatgct gattgccgat gaagtgcaga gcggcgcggg gcgtaccggc acgctgtttg
     7981 cgatggagca gatgggcgtg gcgccggatc ttaccacctt tgcgaaatcg atcgcaggcg
     8041 gtttcccgct ggcgggcgtc accgggcgcg cggaagtgat ggacgctgtt gcaccgggcg
     8101 gtctgggcgg cacctatgcc ggtaacccga ttgcctgcgt ggctgcgctg gaagtgttga
     8161 aggtgttcga gcaggaaaat ctgctgcaaa aagccaacga tctggggcag aagctaaaag
     8221 acggattgtt ggcgatagcc gaaaaacacc cggagatcgg cgacgtacgc gggctgggat
     8281 cgatgattgc catcgagctg tttgaagatg gcgatcacag caaaccggac gcaaaactca
     8341 ccgccgagat cgtggcacgc gcccgcgata agggcctgat tcttctctcc tgcggcccgt
     8401 attacaacgt gctgcgcatc cttgtaccac tcaccattga agacgctcag atccgtcagg
     8461 gtctggagat tatcggtcag tgttttgatg aggcgaagca gtagcaccgc ccccatgcag
     8521 gaggcgacgc tgcgcgtctt gtccggccta cgagaatcca agtcggataa ggcgtttacg
     8581 ccgcatccga caatctgtac gtgaacagga ataaatctat gtcggccgga taaggcggag
     8641 ccgctctccg gcaaaaagaa tcaataacaa ttaaacgcgt gacccgacgc gggaaatgtc
     8701 ggggcgctct cccaagtgac acactttcga gaggattcag gatggggcaa tcatcgcaac
     8761 cacacgagtt aggcggcggg ctgaaatcac gccacgtcac catgttgtct attgccggtg
     8821 ttatcggcgc aagtctgttt gtcggttcca gcgtcgccat cgccgaagcg gacccggcgg
     8881 tattactggc ctatctgttc gccggattac tggtggttat gattatgcgg atgctggcgg
     8941 aaatggcagt tgctacgccc gataccggtt cgttttccac ctatgccgat aaagccattg
     9001 gccgctgggc gggctatacc atcggctggt tgtactggtg gttttgggta ctggttatcc
     9061 cactggaagc caacatcgcc gccatgatcc tgcactcgtg ggttccaggc attcccatct
     9121 ggttattttc cctcgtcatt accctcgcct taactggcag taatttatta agcgttaaaa
     9181 actacggcga atttgagttc tggctggcgc tgtgcaaagt catcgctatc ctggccttta
     9241 ttttccttgg tgcagtcgca attagcggtt tttaccctta tgccgaagtg agcgggatct
     9301 caagattgtg ggatagcggc ggctttatgc ccaacggttt cggtgcggta ttaagcgcga
     9361 tgttgatcac catgttctcg tttatgggcg cagaaattgt caccattgcc gccgcggaat
     9421 ccgacatgcc ggaaaaacat attgtccgcg ccaccaactc ggttatctgg cgtatttcta
     9481 tcttctattt gtgctctatt tttgtcgtcg tggcattaat accgtggaat atgccggggc
     9541 tgaaagccgt tggatcttat cgctcggttc tggaattgct caatattccc catgcgaaat
     9601 taatcatgga ctgtgtgata ttactttccg taaccagttg cctgaactcg gccctgtata
     9661 ccgcctcaag gatgctctac tccttaagcc gtcgcggtga tgctcccgcg gtaatgggta
     9721 aaatcaaccg cagtaaaacc ccgtacgtgg cggtgttact ctccaccggc gcagcattct
     9781 taacggtggt ggtgaactat tacgcgcctg cgaaggtatt taaatttctg atcgacagct
     9841 ccggcgctat cgccctgctg gtttatttag tcatcgccgt ttcacagttg cggatgcgca
     9901 aaattctgcg agcagaagga agcgaaattc gcttgcgaat gtggcttgat ccctggctca
     9961 cctggctggt catcagcttt attacctttg tgttggtagt gatgctattc cgtccggcgc
    10021 aacagttaga agtgatctcc accggcttat tagcgatagg gattatctgt accgtgccaa
    10081 ttatggctcg ctggaaaaag ctggtattgt ggcaaaaaac acccgttcat aatacgcgct
    10141 gataatgatc aggagtcaca ccatgaccat tacgtctctg gatggctatc gctggctgaa
    10201 gaacgatatt attcgcggta attttcaacc ggatgaaaaa ttacggatga gtttactgac
    10261 atcgcgttat gcacttggcg ttgggccgtt gcaggaagca ctttctcaac tggtggcgga
    10321 acggctggtc acggtggtga atcaaaaagg gtatcgggtg gcgtctatgt cagagcagga
    10381 gctgctcgat attttcgacg cccgcgccaa tatggaagcg atgctggtca gtctggcgat
    10441 tgcccgcggt ggcgatgaat gggaggcaga cgttctcgca aaagcgcatc tgctcagtaa
    10501 gctggaagcc tgtgacgcca gcgagaaaat gcttgatgag tgggatctgc gtcatcaggc
    10561 gtttcataca gcaattgtgg cgggctgtgg ttctcactat ttgctgcaaa tgcgtgaacg
    10621 gttgtttgat ctggcggcgc gttatcgatt tatctggctg cggcgaacgg tgctttcggt
    10681 ggaaatgctg gaggataaac acgatcagca ccagaccctg actgcggcgg tactgccgcg
    10741 agatacagcg cgcgccagtg agttaatgcg ccagcattta ctgacgccaa ttcccattat
    10801 ccagcaggcg atggctggca attaactact cttccggaat acgcaacact tgccccggat
    10861 aaattttatc cgggcttttt agcatcggtt tattcgcttc gaagatttta ttgtacagat
    10921 tagcgttacc gtagacctgt ttggaaatgg cactcagagt gtcgccagac ttaacggtat
    10981 aaaactggct ggcagtggct ggtgtcgccg ttttcacctg atcatcaaca ctggcaatac
    11041 cggaaatatt ccccaccgca acaaggattt tctccttcgc ctcctgactc aggccgtcac
    11101 cagtgaccgt cgctttgccg tcggcaattt gaatgttcac tttatcggca tcaggtatac
    11161 cggttttgct cagatgctcc tgcaccttct tcgcctgatc gtctttatcg tgctgacctg
    11221 taaccgcatc ccagagtttt tctccggcat ctttaacaaa attgaacaga cccatagcta
    11281 cctcattcgt taacggaaac actcagaaag tgtagcagag gaacgcggcg cgttaacgcc
    11341 gcgggggtga gcacaggttt aatcgcgggt ttgcacccag aacgcgtgaa tcaaaccggg
    11401 gatataccca agcagtgtca gcagaatatt aataatgaac gcccaaccga accctttacc
    11461 gagcagcacg ccgatcggcg gcagaatgat ggtgatgacg attctccaga aacccatatg
    11521 tactccctat aagaaaatta ctcattgttt aaaaagagat tttatctctt aagcgtagta
    11581 attttagcgg agcctgccag t
//
LOCUS       DAEEUE010000115        11303 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.115, whole genome shotgun sequence.
ACCESSION   DAEEUE010000115 DAEEUE010000000
VERSION     DAEEUE010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11303
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.115"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="KLK84_003410"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="KLK84_003410"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HBE2010609.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="KLK84_003411"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="KLK84_003411"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HBE2010610.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="KLK84_003412"
     CDS             complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="KLK84_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HBE2010611.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEINGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="KLK84_003413"
     CDS             6038..6658
                     /locus_tag="KLK84_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBE2010612.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="KLK84_003414"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="KLK84_003414"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HBE2010613.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="KLK84_003415"
     CDS             complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="KLK84_003415"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBE2010614.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9035..9403)
                     /gene="folB"
                     /locus_tag="KLK84_003416"
     CDS             complement(9035..9403)
                     /gene="folB"
                     /locus_tag="KLK84_003416"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HBE2010615.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9508..10125
                     /gene="plsY"
                     /locus_tag="KLK84_003417"
     CDS             9508..10125
                     /gene="plsY"
                     /locus_tag="KLK84_003417"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBE2010616.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="KLK84_003418"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="KLK84_003418"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
BASE COUNT         2654 a         3250 c         2781 g         2618 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcagcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccactg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccattgat
     5401 ctctactagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaagg
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct atgccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtgtcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaaccgtc ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc
     8041 ggtgcgaaca atcaccccca ccccggccct ctccctacag agcgagggag aaaacccggc
     8101 ctgctcccga accttttctc aatcaaaaga acacgacata caccgcagcc gccacaataa
     8161 agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa
     8221 tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc gggatatcgc
     8281 cggttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg
     8341 gtaccgccag caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc
     8401 cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga cactggaaac
     8461 agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt
     8521 tcggcttcag gcattcggcg gcaatcagca acaaaccgcc aacgaccagc gcatacatca
     8581 catttatcgg gttaaacaat gacttaatcg tgtcgtggaa taatagcccc agtaccaccg
     8641 ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgctt tcaccttcgt
     8701 gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca
     8761 ctactaccgc cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc
     8821 cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa
     8881 attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc
     8941 tcatcaataa acccctaaat tattaaaatg taccgcttgt ccgaactact gcagtatgac
     9001 caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc
     9061 cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc ttgctgagtt
     9121 tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca
     9181 cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt tctgcaatgt
     9241 cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg
     9301 ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa
     9361 tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt
     9421 ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga
     9481 acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc
     9541 gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc
     9601 gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc
     9661 aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt gcctgtctgg
     9721 ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat cgccgcctgt
     9781 cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt tgctaccgct
     9841 tttggagcaa tcgcaccaat tagctgggat ctcaccggag taatggcggg aacctggtta
     9901 ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct
     9961 ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc
    10021 ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa
    10081 atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta
    10141 catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa
    10201 ttccgcgcag acacgaattt catgctgcga tagagcagct cccggtaaac agcccagata
    10261 ttggcgcttt gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc
    10321 aacacatccc actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact
    10381 atctcgccgc tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg
    10441 ttccccaact cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga
    10501 cgacttaatt cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg
    10561 cataatattc ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca
    10621 ttaatacgaa tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat
    10681 tcaaaatgca cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata
    10741 tggctgcgcc caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg
    10801 atttgcgtga cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg
    10861 taacaacgtt ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt
    10921 gttgccagcg tattttcaag gatttggatc cgcttggtga caaatgcagg cgtttgccca
    10981 agcgtcgccg ctgcagcact aaaactcccg ctgtggacga tttctaccag tacctgcaag
    11041 tctttggcta aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag
    11101 tgtatttcgc aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg
    11161 ttgattcacg aactgttaat tacatattca cggtggcaaa aaatataaaa ccacattttt
    11221 agtgttagtt tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat
    11281 gagcgaaagt aataagcaac agg
//
LOCUS       DAEEUE010000116        11084 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.116, whole genome shotgun sequence.
ACCESSION   DAEEUE010000116 DAEEUE010000000
VERSION     DAEEUE010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11084
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.116"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            404..562
                     /locus_tag="KLK84_003419"
     CDS             404..562
                     /locus_tag="KLK84_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBE2010617.1"
                     /translation="METLSTNLQLARLVGVQGTPATIIGDEMIPGAVSWETLEAVVKE
                     KLAVAHAQ"
     gene            552..1058
                     /locus_tag="KLK84_003420"
     CDS             552..1058
                     /locus_tag="KLK84_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003846856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HBE2010618.1"
                     /translation="MLSKLRRWLREGAILLVVLAGVIILLDVWRSPQMPAMFDSTPLH
                     TLDGETVTLASISEERPVLLYFWASWCGICRFTTPDVARLQSEGESVMTIALRSGNDG
                     EVSRWLSRKRVTFPVVNDSGGEISRNWEISVTPTLVVVSKGQVVTTTSGWTSYWGMKL
                     RLWRAAMF"
     gene            1241..2056
                     /locus_tag="KLK84_003421"
     CDS             1241..2056
                     /locus_tag="KLK84_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HBE2010619.1"
                     /translation="MIRSGDFFDFHFDRDNNSMRKGKIIVASMMVLCVLIFVAAAAWF
                     MLFQGEMTEPTEEEKQAAILHAAGLMKSETQDKKSVETLYHRYIIQRHVNLDSGELVA
                     GSSADTARQKCEKLAPERDPAQVRQRCTVADVFFVKDKNNEIQQVIIPVTGKGAKSMM
                     HAFLALGLDGRTVRNLYYYQQRETPFLGARVEDANWRKQWPGKRLLDNSGHPALKIVQ
                     DKPEHADEYTVDGISGATLTSTGVEKSINYWMGPQGYGQFLQRLASDRNNLNL"
     gene            2403..4289
                     /locus_tag="KLK84_003422"
     CDS             2403..4289
                     /locus_tag="KLK84_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HBE2010620.1"
                     /translation="MSVWASTNYAPLIEDIEQRLDKTAELYQQQHADEARRTVQMAYF
                     EVFENLEGPIRINISARKSYEMESAFGEIRRMIGEKKPLADVQARIDWLKAALREVEP
                     VLDGGHRLVAEEQHNALSRDDIAVHWQESFRTIDDLLAQAVTEYQAGNYSVASQHVQQ
                     AHYQGFKNSEMEMSLRQNRSAKDAASINQQFSSLIALTAQPDHLNDVSYQVTTLLQDI
                     EDILPGLPTTRDDQQVAAAQSADNIPAADEGNNRTDWAEVTTGINQSIQDAIARYQGG
                     DAKNAILDIQDTYFDRFEASGMENKIGSRDSAFKTTLEAYFTRLVSLMKAGQPVERLN
                     AEASALTQDLQKAVTMLGEGEETQWSLLLYSLMIIVREGLEALLIVAAIVAYMVKNNH
                     QDKLPLIRQSVIVALIASVITAAIFQMLFTNSGASRELLEGITMLIAVVMLFFMSYWL
                     LSKVEARHWKAWLEGKLSHSLSRGSLVGLWLTSFLAVYREGAETVLFYYALIGDANTV
                     SGHMAIGAGFVIGCVVLLLAWLVMRYSVVRLPLKPFFMFTGSFMYLMAFVFAGKGVLE
                     LVEGKLFQPTLINGFPEISWLGIYPYVETLLPQVVLLLAALVALWVMRRKSAVPGETI
                     KNNP"
     gene            4330..4857
                     /locus_tag="KLK84_003423"
     CDS             4330..4857
                     /locus_tag="KLK84_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016674077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HBE2010621.1"
                     /translation="MTMKKTLIASAVMASIFIAPAAFAFKEYPAGEPVTMNEMELAAV
                     YLQPIDMEPRGMGLPAAKADVHLEADIHAVEGNKNGFGAGEWIPYLTISYTLVNNDTG
                     EKQEGTFMPMVASDGPHYGANIKMMGVGNYKVTYHIEPPSKAGMHRHTDSETGVGRWW
                     KPFDVSYEFKYVGLN"
     gene            4961..6340
                     /locus_tag="KLK84_003424"
     CDS             4961..6340
                     /locus_tag="KLK84_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003836820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2318 domain-containing protein"
                     /protein_id="HBE2010622.1"
                     /translation="MSYFFVTTLQVFFCIALLSGVLWSRNDPPSLRPLTWTLLTGLIA
                     GVLAGLFIHGSQPVQLLLVGAEVMVSLLFVLSFWWASTRIRYLWQGILIFGAARHWAL
                     DPNLGGLTSTHVLNTDLLLNLTAVVLAFAILCLAGVLCAMLLRRIRGLYWPLTLILLV
                     MIWLPLSGNLLLLLMKLQVVPLGKSLLSFVAKVTNNTALYNWAGAALLLALALCWLPA
                     LLRAFRQTRETEEPIAHRLALAQRRNALRLWLVTIGCAVVVIAGQLWWDKVASQPPQL
                     SEAVPVTLGSDGMVRLPVEQLRDGKLHRFVWVADDGKAVRFFVINRYPDKLRFGVVFD
                     ACLLCGDQGYVMEGNQVICVACGVHIFIPSIGKAGGCNPVPIENWHNDEKELVIPGKE
                     LATGVNYFSTVMTIKVTDPVDGSTLTNTSADYKYSYGGKTWFFSSEANYERFRETPEQ
                     FVPADMREE"
     gene            6343..7626
                     /locus_tag="KLK84_003425"
     CDS             6343..7626
                     /locus_tag="KLK84_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE2010623.1"
                     /translation="MLWRMLRQSWGRNLRRKVLAIITVFLASSLISALLAVSIDIGDK
                     MSRELKSYGANILIEPAGQAALPALFSESSNPLSGQDFLDEAELPNIKDIFWRNNIVG
                     FAPMLGGEASVEGEPVRILGTFFSQPVDIPDEEGYETGQKTVSPYWQVTGDWPQEPAG
                     AEPQTLVGHALARQMGWKPGDKLTLRTEGEAVQVTVSGILSSGGDEDNQLVMPLSTVQ
                     HLLGLPGKVQAIRVSALTVPENELSRRARENLDALNAEEYDLWYCTAYVSSIAHQLEE
                     AISGAEVRPVWQVAASEGVVIDKIQLLMAVVTVAALVASAMGIASLMTSTIMERAKEI
                     GLMKALGARQWQIMLLFYLEAASSGLAGGALGCIAGWGLAKAIGVMLFDAPLNFAWIV
                     VPCVLVIAVLIALIGTWFPARRIARLYPVEVLYGR"
     gene            7616..8746
                     /locus_tag="KLK84_003426"
     CDS             7616..8746
                     /locus_tag="KLK84_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE2010624.1"
                     /translation="MAAKRTMLWLLVWRALRLRFQRVSVVFAALMVGATIVTALSAVW
                     FDINTKMSEELRTFGANFYIGPGHGSSMPQQELQSILDQAPQGLVHGASPWLYGMART
                     ELEKVVMVGVWFESLQKMVPYWQVTGSWIGVSFDDRNAMIGVKLAERLNVQPGDSITL
                     VDHNQRKNLQIKGIVESGDATDNMLIVSLDVAQAWLHQPGKISHGLLSVSNDVGQVEN
                     YASRLQAQYPDLEIRPVRKVSASEGQVLDKIKGLMGLVSIVILALSSLCVNTTLMAIV
                     GERAREFALQKALGSSNGDIVRQILLETSIIALAAVACGWVLGYLLAQLLGLTVFNAA
                     ISLRLPVLPITLVLSLLVAILAAIVPVRRAVSVEPAKVLKGE"
     gene            8751..9446
                     /locus_tag="KLK84_003427"
     CDS             8751..9446
                     /locus_tag="KLK84_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004863043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE2010625.1"
                     /translation="MSVTHIPQVAIETRHLYKRFGDVTALDDINLRIAQGEFVAIMGA
                     SGSGKTTLMNILTCLDTATEGQVFLDGTDAAALDEEGRRRFRAEKIGLVFQQFHLIPF
                     LTALENIMLAQHYHSVVDEAAARKVLEQVGLGHRVTHLPSQLSGGEQQRVCIARALVN
                     EPPVIFADEPTGNLDEENEQRVLDLLTDLHRQGRTIVMVTHNPALGQFADRILRLQHG
                     KYLGEEANQHALA"
     gene            9433..9918
                     /locus_tag="KLK84_003428"
     CDS             9433..9918
                     /locus_tag="KLK84_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBE2010626.1"
                     /translation="MRWHNVLLLAAAILISGCKEEKLAVGAPAPALAVFDLQGQESGL
                     ERWQGKAVYLNFWSAGCGGCLAEMDTLEALSKKWGDKVVVVAVNTDPETVRINDLLAK
                     HQITYPVVRDQLNITQERYQVIGTPTSVLIDSEGRVLELHQGMRKPPELEATFARLAA
                     R"
     gene            9943..10428
                     /locus_tag="KLK84_003429"
     CDS             9943..10428
                     /locus_tag="KLK84_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010627.1"
                     /translation="MAFIWNDESLAILRENAGILTTEQIAQLLHTNITAVRNMAYRLK
                     LSLRVTAYNHRRIAQVQALYASETLSLKEIAAKTGLTASTVQYIVYVKSKNKPYATTE
                     YVSFETENAVHYRVQKEFVDTERSLLDNISDNTRFRELYLTDGTFYCARNIKYEVFIS
                     E"
     gene            10550..>11084
                     /locus_tag="KLK84_003430"
     CDS             10550..>11084
                     /locus_tag="KLK84_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine-type phosphatase"
                     /protein_id="HBE2010628.1"
                     /translation="MKKLILAAAVAGAVLLSSAAQAQTTPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNQWSEWDVPGGQLTTKGGVLEIYMGHYMREWLAELGMVTSGECP
                     TPDTVYTYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SQKAVQAMEKERSQMQLD"
BASE COUNT         2455 a         2738 c         3335 g         2556 t
ORIGIN      
        1 tcggggcaga attggtaaag agagtcgtgt aaaatatcga gttcgcacat cttgttgtct
       61 gattattgat ttttcgcgaa accatttgat catatgacaa gatgtgtatc caccttaact
      121 taatgatttt taccaaaatc attaggggat tcatcagggt gatgcgtgca ctacgcaaag
      181 gccaggcctc agcattttat tatggtgatc ccctgggcga aatgcgcctg gtaagcagag
      241 tttttgaaat gtaaggcctt tgaataagac aaaaggctgc ctcatcgcta actttgcaac
      301 agtgccgtgc agacagaaac cgtaaacacc ggcattgaca tgccggggaa gcccttatag
      361 tacaataatt ctctatatcc aaactgaccc cgatgagaaa agcatggaga cgttgagcac
      421 caatctgcaa ctcgcccggc tggttggcgt tcaggggacg ccggcaacca tcattggcga
      481 cgagatgatc cccggcgcgg tatcgtggga gacccttgag gcggtggtga aagaaaaact
      541 ggcggtggcc catgctcagt aaactgcgcc gctggctgcg tgaaggcgcg atcttactgg
      601 ttgtgctggc tggcgtgata atcctgctgg atgtctggcg ttctccgcag atgccggcca
      661 tgtttgacag cacgccgctg catacgctgg acggggaaac cgtaacgctg gcgtcgatca
      721 gtgaagaacg cccggtgctg ctctacttct gggcgagctg gtgcggtatc tgccgtttca
      781 ccacgccgga tgtcgcccgt ttgcagtcgg aaggggagag cgtcatgacc atcgcgctgc
      841 gttccggtaa tgacggggag gtttcccgct ggttatcgcg taaaagggtg acgttccccg
      901 tcgtgaatga tagcggcggt gagatatcac gaaactggga aatcagcgtc acgccaacgc
      961 tggtggtggt gtcgaagggg caggtggtaa ctacgaccag cggctggacc agctactggg
     1021 gcatgaagtt acgtctgtgg cgggcagcca tgttttgata gcgtgccggg gaaacccggc
     1081 attttttaac ccattgtaag gatgtgtaaa acgagcggag agcgtgtttt ctgttatttt
     1141 tgctgctttt ttaccgacgc cagttattat tttttaccgc tgcctgacgt tgcaaacctg
     1201 aattgataat ggcatatctg ttttaattga atgaaagtct gtgatacgtt caggcgattt
     1261 tttcgatttc cattttgaca gggataacaa ttcaatgcgc aagggtaaaa ttattgttgc
     1321 atcaatgatg gtgctttgtg tccttatttt tgttgcggcg gcggcatggt ttatgctgtt
     1381 ccagggagaa atgacggagc cgacagagga ggaaaaacag gcggcaattt tacacgctgc
     1441 gggcctgatg aaatcagaga cccaggataa aaaatcagtc gaaacgctct atcatcgtta
     1501 tattattcaa cgccatgtta atttagattc cggcgaactg gtggctggca gcagcgcgga
     1561 caccgcgcgg cagaagtgtg aaaaacttgc ccctgaacgc gacccagcgc aggttcgtca
     1621 gcgctgtacg gtggcggatg tgttctttgt gaaagataaa aataatgaaa ttcagcaggt
     1681 tatcattcca gttaccggta agggtgcgaa atccatgatg catgcgtttc ttgctctggg
     1741 gctggacggt cgtacggtga gaaatctgta ttattatcag caacgggaaa cgccgtttct
     1801 gggggctagg gtggaagacg ccaactggcg caaacagtgg ccagggaaac gattattaga
     1861 taacagcgga catcctgcat tgaaaatagt acaggataaa cctgaacatg ccgatgaata
     1921 taccgttgac ggtatttccg gtgcgacgtt gacatcaacc ggcgttgaga aaagtattaa
     1981 ctactggatg gggccgcagg gatatggtca attcttacag cgtcttgcca gcgatcgaaa
     2041 taaccttaat ctttgaatat tgctgtacgc aataacgcgt tttatttctg cctgccgata
     2101 atgaataata tcactgtcgg cagggggatt tattcctctg ttaccgcgcc tgccttgccg
     2161 ttaattccat gagaaaaaca gggagataac gtccctttta tcaacgcata atgtgtgtgt
     2221 tcatcacgat ttgatgaaat aagtttacat aacttgtcgt ctgctgacat ttttatgatc
     2281 caggtcatgt ataacgcgac aaaaagatat gataataatt atcatttgta ttctttggcg
     2341 gtgtgcacgt gcgtaacttc cgtaactctt tgttggttac tctctttggc ttgtttataa
     2401 gcatgagtgt ctgggcgtcg acgaattatg cgcctttgat tgaagatatc gagcaacgtc
     2461 ttgataaaac agcggaactt tatcagcaac agcatgccga cgaggcgcgc cgcacggtac
     2521 aaatggccta ttttgaggtg ttcgagaacc tggaagggcc gatccgcatc aacatttccg
     2581 cgcgcaaaag ttacgagatg gagagcgcat tcggcgagat ccgtcgaatg attggcgaga
     2641 aaaaaccgct cgctgacgtc caggcgcgca ttgactggct gaaagccgca ctgcgcgaag
     2701 tggaaccggt gctggatggc ggacatcgtc tggtggccga agagcagcac aacgcgctgt
     2761 ccagggacga cattgccgtt cactggcagg agagtttcag gacgatcgac gatctgctgg
     2821 cgcaggcggt cactgaatat caggccggga attacagcgt tgccagtcag cacgttcaac
     2881 aggcgcacta tcagggcttt aaaaactctg agatggagat gtccctcaga caaaaccgtt
     2941 ctgcaaaaga tgccgcctcc attaaccaac aattctcatc cctgattgcg ctgaccgcgc
     3001 aacctgacca tctcaacgat gtctcctatc aggtcactac gctgctacag gatatcgagg
     3061 atatcttacc cggcttgcca accacccgtg acgatcagca ggttgccgca gcgcaaagcg
     3121 cagacaacat tccggcggcg gacgagggaa ataaccgcac cgactgggct gaggtgacga
     3181 cgggaattaa ccagagcatt caggacgcga tagcgcgcta tcagggcggc gatgcgaaga
     3241 atgcgattct ggatatccag gatacctact tcgaccgctt cgaagccagc ggcatggaaa
     3301 acaaaatcgg ctctcgcgat tcggcattta aaaccacgct ggaggcgtat tttacccgcc
     3361 tggtcagtct gatgaaggcc ggtcagccgg ttgagcgcct gaatgcagag gccagcgcgc
     3421 tgacgcagga tctgcaaaaa gccgtgacga tgctgggcga aggcgaagaa acccagtgga
     3481 gtctgctgct ctatagtctg atgatcatcg tgcgcgaggg gctggaagcg ctgctgattg
     3541 ttgcggcaat cgtggcctac atggtgaaaa acaaccatca ggataagctg ccgcttattc
     3601 gtcagtcggt aattgtggcg ctgatcgcca gcgtcatcac cgccgctatc ttccagatgc
     3661 tgtttactaa ctccggcgcc agccgtgaac tgctggaagg cataacgatg ctgattgccg
     3721 tcgtgatgct gttctttatg agttactggc tgctgtccaa agtggaagcg cgccactgga
     3781 aagcctggct ggaaggcaaa ctgtctcatt ccctgtcgcg aggctccctg gtcggactat
     3841 ggctgaccag cttcctggcg gtgtaccgcg aaggggcgga aacggtgctg ttctattacg
     3901 ctctgattgg cgatgcaaac acggtatccg gccatatggc gattggcgcc ggttttgtga
     3961 ttggctgcgt ggtgctgctg ctcgcctggc tggtgatgcg ttactcagtg gtgcgcctgc
     4021 cgctgaaacc cttctttatg tttaccggca gtttcatgta tctgatggcc ttcgtctttg
     4081 cgggcaaagg cgttctggaa ctggtggaag gcaaactgtt ccagcctacg ttgattaacg
     4141 gcttccctga aatcagctgg ctggggattt atccctacgt ggaaacgttg ttaccgcagg
     4201 tcgtattgct gctcgcggcg ctggtggcgc tgtgggtgat gcggcgcaaa agcgcggttc
     4261 ccggggagac gataaaaaat aacccataat cgttgtatag ccgttgtttt agacgagagg
     4321 atacgtttga tgaccatgaa gaaaaccctg attgccagcg cagtgatggc cagcattttc
     4381 attgcgccag ccgcgtttgc ttttaaagag tacccggcag gcgagcctgt caccatgaat
     4441 gaaatggagc tggcggccgt ttacctgcaa ccgattgata tggaaccgcg cggcatgggc
     4501 ttaccggcag caaaagccga tgttcacctt gaagcggata tccacgctgt agaaggcaat
     4561 aaaaacggct ttggcgcagg ggaatggatc ccgtacctga ccatcagcta caccctggtc
     4621 aacaacgaca cgggcgaaaa acaggaaggc accttcatgc cgatggttgc cagcgatggc
     4681 ccgcactatg gcgcgaacat caaaatgatg ggcgtgggca actataaagt gacctaccac
     4741 attgaaccgc cgtcaaaagc cgggatgcat cgccatactg acagcgaaac aggcgtaggc
     4801 cgctggtgga aaccgtttga tgtgagctac gagtttaaat atgtcggtct gaactaaaac
     4861 cgactaacca acgcgccagg aggattcccc cttctggcgc gttggtcaga ccgatcatat
     4921 gcccgtcgct taattcaacc cgccgtacag agtcgaagtt atgagttact ttttcgtcac
     4981 gacgctacag gtctttttct gcattgcgct tctttccggt gttctctgga gtcgaaacga
     5041 tcctccctct ttacgtccgc tgacctggac gctgctgacc gggctgattg ccggcgtgct
     5101 ggcgggactg tttattcacg gcagccaacc ggtacaactg ctgctggttg gggcggaggt
     5161 catggtatcg ctgctgtttg tcctcagttt ctggtgggct tcaacgcgta tccgctatct
     5221 gtggcagggg attctgattt tcggcgctgc ccgccattgg gcgctggacc ctaatctggg
     5281 cgggctgacc agtacgcatg tgctgaatac tgacctgctg ttaaacctga cggccgtcgt
     5341 tctcgctttt gcgatcctct gtctggcggg cgtactctgc gcgatgctgc tgcggcgtat
     5401 tcgcggcctg tactggccgt taacgctgat tctgctggtg atgatctggc tgccgctgag
     5461 cggcaacctg ctgttgttgc tgatgaaatt acaggttgtt ccgctgggaa aatcactgct
     5521 gagcttcgtg gcgaaagtca caaataacac ggcgctgtat aactgggcgg gagccgcgct
     5581 gctgctggcc ctggcgctgt gctggcttcc ggcgctgttg cgggcttttc gacagacgcg
     5641 ggaaaccgaa gaaccgattg ctcaccgtct ggcgctggcg cagcgtcgca acgctttgcg
     5701 gctgtggttg gtcacaatcg gctgcgcggt ggtcgttatc gcaggccaac tgtggtggga
     5761 taaagtggcc tcgcagccgc cgcaactctc tgaagccgtg cccgtaacgc tggggagcga
     5821 tggcatggtg cgcctgccgg ttgaacagct tcgtgacggt aaactgcatc gtttcgtctg
     5881 ggtggcggac gatggcaaag cggtgcgctt cttcgtgatt aaccgctatc ccgacaagct
     5941 gcgttttggc gtggtgttcg acgcctgcct gctgtgcggc gatcagggtt atgtcatgga
     6001 gggcaatcag gttatctgcg tcgcctgcgg agtgcatatt tttattccgt ctatcggcaa
     6061 ggccggtggc tgtaacccgg tgccgattga aaactggcac aatgatgaaa aagagctggt
     6121 gatccccggt aaagaactgg cgacaggggt gaactacttc tcgacggtca tgactattaa
     6181 agtgaccgat ccggtcgatg gttcaacgtt gaccaatacc tcagccgatt acaaatacag
     6241 ctatggcggc aagacctggt tcttctcgtc agaggctaac tatgaacgct tccgtgaaac
     6301 gccggagcag tttgttcctg ccgacatgag ggaggaataa tcatgctgtg gcggatgtta
     6361 cgacaatcct gggggcgcaa tttacgccgc aaagtgctgg cgataatcac cgtttttctg
     6421 gcctccagcc tgatctcggc gctgctggcg gtgtccattg atatcggcga caaaatgtcc
     6481 agagagctga agtcttatgg tgcgaacatt ttgattgaac ccgccggaca ggctgcgctg
     6541 cccgcgctgt ttagtgagag cagtaatccg ctttccggtc aggattttct tgatgaagcc
     6601 gagctgccga acatcaaaga tatcttctgg cgaaacaaca ttgtcgggtt tgcgccgatg
     6661 ctcggcggcg aagccagcgt ggagggagaa ccagtgcgga ttctgggcac tttcttcagt
     6721 cagccagtgg atattcctga tgaagaggga tatgagacgg ggcagaaaac ggtcagccca
     6781 tactggcagg tgaccggcga ctggccgcag gaacccgcag gcgcagagcc gcagacctta
     6841 gtcgggcacg cgctggcgcg gcaaatgggc tggaaacccg gtgataagct gacattacgt
     6901 accgaaggcg aagcagtgca ggtcaccgtc agcggtatcc tctccagcgg cggcgatgaa
     6961 gataaccaac tggtgatgcc gctaagcacg gtacagcacc tgctggggct ccccggaaaa
     7021 gtgcaggcga ttcgcgtctc ggcgctgacg gtgccggaaa acgaactgtc ccgccgcgcg
     7081 cgggaaaacc tggacgcgct caatgccgaa gagtacgacc tctggtattg cacggcctat
     7141 gtgtcgtcga tcgcgcatca gctggaagag gcgatttccg gtgcggaagt gcgcccggta
     7201 tggcaggtcg cggcgtccga aggggtggtg atcgataaaa ttcagttgct gatggcggtc
     7261 gttaccgtcg cggcgctggt ggcttccgca atggggatcg cctcgctgat gaccagcacc
     7321 attatggaac gcgccaaaga gatcggtttg atgaaagcgc tgggtgcgcg acagtggcaa
     7381 attatgctgc tgttctacct ggaagcggcg tccagcgggc tggctggcgg tgcgctggga
     7441 tgtattgccg ggtgggggct ggcgaaagcg attggcgtca tgctcttcga cgcgccgctt
     7501 aattttgcct ggattgtggt gccgtgcgta ctggtgattg cggtactgat tgcgttgatc
     7561 ggcacctggt tcccggcccg gcggattgcc cggctgtatc ctgtggaggt gctgtatggc
     7621 cgctaaacgt accatgctct ggctgctggt gtggcgggcg ctgcgtctgc gttttcagcg
     7681 ggtcagcgtg gtgtttgccg cgctgatggt gggggcgacc atcgtgacgg cgctttctgc
     7741 cgtctggttc gacattaaca ccaaaatgag cgaagaactg cgcaccttcg gcgcgaattt
     7801 ttacatcggg ccggggcacg gttccagtat gccgcagcag gagttgcaga gcattctcga
     7861 tcaggcgccg caggggctgg tgcatggcgc cagtccgtgg ttgtacggca tggcgcgtac
     7921 cgagctggaa aaagtggtga tggttggcgt gtggttcgag tcgctgcaaa aaatggtgcc
     7981 gtactggcag gtcaccggta gctggattgg cgtcagtttt gacgaccgta atgcgatgat
     8041 cggcgtcaaa ctggcggaac gcctcaacgt gcagccgggc gacagcatca cgctggtgga
     8101 tcacaatcag cgtaagaatt tgcagattaa aggcattgtg gagtctggcg atgcgacgga
     8161 caacatgctg atcgtcagcc tcgacgtggc gcaggcctgg ctgcatcagc cgggcaaaat
     8221 cagccacggc ttgctgagcg tcagtaacga tgtcggccag gtggagaact atgccagccg
     8281 gttgcaggcg cagtatcctg acctggagat tcgtccggtg cgtaaggtct ctgcttccga
     8341 agggcaggtg ctggataaaa ttaaaggtct gatgggactg gtctcgatcg tgattctggc
     8401 gctctcttcg ctgtgcgtga ataccacgct gatggcgatt gtcggcgagc gggcgcgcga
     8461 gtttgcgctg caaaaagcgc ttggttccag caacggcgat atcgtgcggc aaattctgct
     8521 ggaaaccagc atcatcgcgc tggcggcggt ggcgtgcggc tgggtgctgg gatatttgct
     8581 ggcgcaactg ttgggattaa cggtgtttaa tgccgccata tcactgcgtc tgccggtgtt
     8641 gcccattact ctggtgttgt ctttactggt cgccattctg gcggcgattg ttccggtgcg
     8701 acgcgcggta agcgtcgagc ctgctaaagt gctgaaagga gagtagcgcc atgtccgtta
     8761 cgcatatccc tcaggtggcg atcgaaaccc gccacttata caaacggttc ggtgacgtta
     8821 ccgccctgga tgacattaat ctgcgcattg cgcagggtga atttgtcgcc attatgggcg
     8881 cttctggttc tggtaaaacc acgctgatga atattctgac gtgccttgat acggcgacgg
     8941 aagggcaggt gtttcttgat ggcaccgatg ccgccgcgct ggatgaagag gggcgccgtc
     9001 gctttcgcgc cgagaaaatc ggcctggtat ttcagcagtt ccatttgatc ccgttcttaa
     9061 ctgcgctgga aaacatcatg ctcgcccagc attaccacag cgtggtggat gaagcggcgg
     9121 cgaggaaggt gctggaacag gtagggcttg ggcatcgtgt gacgcacctg ccgagccagc
     9181 tttccggtgg tgagcagcag cgtgtgtgta tcgcccgggc gctggtcaac gaaccgccgg
     9241 ttattttcgc ggatgaaccg acgggcaacc tggatgaaga gaatgagcag cgggtgctgg
     9301 atctgctcac cgatctgcat cgtcaggggc gcacgattgt gatggtgacg cacaaccctg
     9361 cgcttgggca gtttgccgac cgtattctgc ggctgcaaca cggtaaatat ctgggtgagg
     9421 aggccaatca acatgcgctg gcataatgtg ctgttactcg cggcggcgat cctgataagc
     9481 gggtgtaaag aagaaaagct ggcggtcggg gcaccggccc cggcgctggc ggtgtttgat
     9541 ctgcaaggcc aggagtcagg ccttgaacgc tggcaaggaa aggcggtgta cctgaatttt
     9601 tggtctgcgg gatgcggggg gtgcctggcg gaaatggaca cgctggaagc gctgagcaaa
     9661 aaatggggcg ataaagtggt ggttgtggcg gtcaataccg accctgaaac ggtcaggatc
     9721 aacgatctgc tggcaaaaca tcagataacc tatccggtgg tgcgcgatca gctcaacatt
     9781 acccaggaac gttatcaggt gatcggcacg ccgacctccg tactgattga ctccgaagga
     9841 cgcgttctgg agctacacca gggtatgcgt aagccgcctg agttagaggc cacgtttgcg
     9901 cgcctggcgg cgcgatgaac gacgcgtacg gagagatacc caatggcatt tatctggaac
     9961 gacgaaagcc ttgcgattct gcgggaaaac gcgggcatcc tcacgacaga gcagatagcg
    10021 caactgcttc acactaatat taccgccgtg cggaatatgg cctatcggct gaaactgagc
    10081 cttcgggtga cggcgtataa tcaccgacgc atagcgcagg ttcaggcgct ttatgcctct
    10141 gagacgttga gtctcaaaga gatcgcggcg aaaacggggc tgacggccag taccgtgcaa
    10201 tatatcgtgt acgtgaaatc aaaaaataag ccctacgcca ccacggaata tgtctctttt
    10261 gaaacggaga acgccgttca ttaccgcgtg caaaaagaat ttgtcgatac ggaacgttcc
    10321 ctgctggaca acatctctga taacacgcga tttcgtgaac tgtatctgac ggacggcact
    10381 ttttattgcg cccgcaatat taagtatgag gtttttatct cggagtaacc ggaacacgct
    10441 gacctgccgg ttatcttctg gaatctcccg ttttgcgaaa ccgttcccgg aaaaatgtca
    10501 tatttctgtc acactctatg aggattgata acaacaagag gttgtaggga tgaaaaaatt
    10561 gatactcgcc gccgctgtgg caggggctgt tttattgtcg tccgccgcgc aagcgcagac
    10621 aacgccggaa ggttatcaac tgcaacaagt gttgatgatg agccgacaca acttgcgcgc
    10681 gccgttggcc aataatggca gcgtgctgga gcagtccacg ccgaatcagt ggtcggagtg
    10741 ggatgtgccc ggcgggcagc tcaccacgaa aggcggtgtg ctggaaattt atatgggcca
    10801 ctacatgcga gaatggctgg cggagctggg gatggtcacg tcaggcgaat gcccgacgcc
    10861 ggacacggta tatacctatg ccaacagtct gcaacgcacc gttgcgacgg cacaattctt
    10921 tatcaccggc gctttccctg ggtgcgacat tccggtacat catcaggaaa aaatgggcac
    10981 gatggaccca acgtttaatc cggtgatcac cgatgactct gcggcgttta gccagaaagc
    11041 ggtgcaggcg atggaaaaag agcgcagcca gatgcagctt gacg
//
LOCUS       DAEEUE010000117        11036 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.117, whole genome shotgun sequence.
ACCESSION   DAEEUE010000117 DAEEUE010000000
VERSION     DAEEUE010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11036
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.117"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            154..504
                     /locus_tag="KLK84_003431"
     CDS             154..504
                     /locus_tag="KLK84_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBE2010629.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            557..952
                     /locus_tag="KLK84_003432"
     CDS             557..952
                     /locus_tag="KLK84_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HBE2010630.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            993..1736
                     /gene="cmoA"
                     /locus_tag="KLK84_003433"
     CDS             993..1736
                     /gene="cmoA"
                     /locus_tag="KLK84_003433"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HBE2010631.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1733..2704
                     /gene="cmoB"
                     /locus_tag="KLK84_003434"
     CDS             1733..2704
                     /gene="cmoB"
                     /locus_tag="KLK84_003434"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HBE2010632.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="KLK84_003435"
     CDS             complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="KLK84_003435"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HBE2010633.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5323..6423)
                     /locus_tag="KLK84_003436"
     CDS             complement(5323..6423)
                     /locus_tag="KLK84_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HBE2010634.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="KLK84_003437"
     CDS             complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="KLK84_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBE2010635.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7571..8137)
                     /locus_tag="KLK84_003438"
     CDS             complement(7571..8137)
                     /locus_tag="KLK84_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBE2010636.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8353..10086
                     /gene="argS"
                     /locus_tag="KLK84_003439"
     CDS             8353..10086
                     /gene="argS"
                     /locus_tag="KLK84_003439"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBE2010637.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10263..10751
                     /locus_tag="KLK84_003440"
     CDS             10263..10751
                     /locus_tag="KLK84_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HBE2010638.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(10869..10937)
                     /locus_tag="KLK84_003441"
                     /pseudo
     CDS             complement(10869..10937)
                     /locus_tag="KLK84_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
BASE COUNT         2691 a         2815 c         2634 g         2896 t
ORIGIN      
        1 taatgggctt ttaatcctat cgtttattgc ttgcatagta gaatctaacc atccatcact
       61 catagctaac cctcggaaat cttacatttc tgtctatcat aaccactagc atcaccttta
      121 ggaaccatct gaaacgggag gtgtatttag accttgctaa tcttagacag ccgcccggtt
      181 catgcatcac gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt
      241 ccggtacatg ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat
      301 atgacgcaaa accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag
      361 accactacgc cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta
      421 cataccctgt gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt
      481 ccacagcaat cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat
      541 tttaaaggga gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat
      601 gaagttctct tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg
      661 tggttttagc gaactgcaaa gcgctattcg cattcatggt aacgcggtgg aatatattcc
      721 catcgcgatt gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca
      781 tatttgcggc atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg
      841 tctgtttcgc tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt
      901 gctggcgaat ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat
      961 acgccacttt ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg
     1021 cccctatcgc cagactgggc gactggacct ttgatgaacg ggtcgctgaa gtcttcccgg
     1081 atatgatcca gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag
     1141 ccgagcgctt cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg
     1201 cgacgctctc ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca
     1261 actccccggc gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc
     1321 cagtagacgt cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg
     1381 tgctgaattt taccctgcaa ttcctggaac cttccgagcg ccaggcatta ctggataaaa
     1441 tttatcaagg actgaacccg ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag
     1501 atgccaaagt tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt
     1561 acagcgaact ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt
     1621 ccgtggaaac ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt
     1681 tccagtgctt taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga
     1741 ctttggtaac ttttattctc tgattgccaa aaatcatctt tcacactggc tcgaaacgct
     1801 gcccgcgcag attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc
     1861 caacacggtg gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt
     1921 aaccgccgaa agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat
     1981 gcggaacctg atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac
     2041 cgaatggcgt tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg
     2101 acgcaccatt ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc
     2161 aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc
     2221 ggtgcgtaaa ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca
     2281 gcttccggca ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg
     2341 ttcgccgctg gagcatctct ggcagttgaa agatcaactg gtgaatgagg gtgaactggt
     2401 gctggaaacg ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta
     2461 cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa
     2521 gaagtgtggt tttgtagata tccgcattgt ggatgtgtgc gttaccacta ctgaagagca
     2581 gcgacgcact gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc
     2641 gagtaagaca gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc
     2701 gtaaaggtct ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct
     2761 tgctaattca atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt
     2821 tttacgtttg cataacctca gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac
     2881 agcaggctta tcaaatgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac
     2941 cagcgcagag ttaccggcac aggcatttgc ccgctgcgag ctggggatat ccgccgttaa
     3001 cacgttcgca ctgccgtttt tacacaagcc gttttccaga tctggccatg caccttcatg
     3061 cacgcatacc acgccttttt tgatcccgtc agtcaccacc gcgcctgtca aaatctgacc
     3121 acgtttgttc cagacacgca ccagatcgcc attcgcaata ccaaatcgag cagcatcttc
     3181 ggtgtgaata gtgatcggtt cacgatcggc aaccgcatat tttttacgca gttccgcata
     3241 gttaagctga ctgtgtaaac ggtgtgccgg atgtgcggtc agaagctgca actgcttctc
     3301 gtcggcggta cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt
     3361 atagccaaat ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag
     3421 cgcatttttc accggatcgg cgcggaaatc accataacga acgtactgtt cgttcttctc
     3481 gctgcggcgc atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt
     3541 gacgcgttgc gcacgggccc ctttctgagc ggcatcatag aaaaatttca gccacgccat
     3601 ttcatcttta ccttcggtat agatctcttt tccgccaggt ttgagtaatt ccgcaagatc
     3661 ggcaaacacg tcaaaatcgt tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg
     3721 cacaatatgc tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt
     3781 ggtgatcggt aatacgatat ctgcgtgttt ggctgctgcg gtccagtagc attcagaaac
     3841 gacgatcatc tccggtttct gccaggcttt gatcagacga ttagtatcct ggtgatgggt
     3901 aaagttaccg ccacccgccc accagatcat tttgatattc ggatacgttt gttctttacc
     3961 gttatgctga tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat
     4021 cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat
     4081 ttccggcagc acgccgccaa cacgcgtcgg gttgccacca ttggagtaat gataagagaa
     4141 accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat
     4201 ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt
     4261 gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat
     4321 ttccgccgtc cagaccgcgc tcttcggcgt attatcgctc ttacctgtca gatactcttc
     4381 aaactgcgga tacccggtag tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt
     4441 catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat
     4501 ccaggtggca ttatcgccaa agaattcgat ggtttcggag cggataggat caatggcaat
     4561 cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact
     4621 gctccaggca atttttaagg tattcagcgg gttcattccc cacagcacca caacctggct
     4681 gttttccaga atcagcggcc aggaggcctg ctgttcatac acctctacag aaccgaccac
     4741 atgcggcatg atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc
     4801 gcccgccagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga
     4861 gcgccagccg taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc
     4921 atgttgctca tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc
     4981 gccacgacct ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg
     5041 ctgaatacgc gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat
     5101 ggttttcgcc agcgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgctcat
     5161 cgctccccaa cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag
     5221 cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc
     5281 acgtcttgtt aatgccataa cttcctccct gatcaacgag gatcactgtt tctcggtaat
     5341 atctttggcg ttgtactgga aataccgcgt taaaatgtcc ggttcgtttt cgctcatgct
     5401 ggttcgtgcc cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc
     5461 ggcaatagag gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca
     5521 tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg
     5581 ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc
     5641 ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc
     5701 actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac
     5761 gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa
     5821 aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc
     5881 cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat
     5941 acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga
     6001 ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt
     6061 taattctttc catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact
     6121 gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta atttagcaaa
     6181 taaataatcc atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt
     6241 ctggttcgaa aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca
     6301 ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc
     6361 gccgccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg
     6421 tatagattcc tctaaataaa ttccagaaca ccgaaatcat tgtataatta aatacacaac
     6481 gaattaaccg cttgcataac tcggcacaac actgcctgaa ataatcgata aagaatatga
     6541 tttattacaa tgtaatcatt aattgctaag gaataaccca gttgctattt agaatatata
     6601 tttatcagtt ttagcaattt aaatcccata atcaatgtga atataaaaaa tatttttagc
     6661 acaacgctat gcgcactgtc acccgctcaa cagatcacgg ttatcgttcg ttttttatac
     6721 tgctcaggga taaaaaaagg cccctgttga aattgcaggg gcctggtacg agcaagcatc
     6781 atattgggcg acatgatgca acggtaaaaa tcatttggcc tgatggcgtt caatgattcc
     6841 tttcatttca gcaaccgccg ccccgtctac gatataacgc gaatactcgt ccgcgtgttc
     6901 atctgatgac atggacaatc cttgattacg ataacgcatc ggtgaggctt gccacgctcc
     6961 cgcggagcta tggacttcca gcactccggc atcgaggaag tggtgcaggt tttctgcacg
     7021 gactcctgct ccagccataa tgattggagc atcacgatgg gcaataagtt ccataatttt
     7081 tgataaacct tgcagtgcgt ctgatttttg ccctgatgtc agtacccggg caatgcccaa
     7141 ttccgcaaga ttattgagtg tatataaagg gttagcgcac atatcgaagg cgcgatgaaa
     7201 agtcactgcc agcggaccgg cagcagccat tattttttcc attcgtggca tatcgacatt
     7261 cccgtcaaca tcgagaacgc ccgtcaccag tccaggaaaa cctaattcgc ggaccgtgcg
     7321 cacatcctca agaatggcgg caaactcacc gtcgctgtag caaaaatcac caccgcgtgg
     7381 gcgaattatc ggatgcacag ggatcgtcac ccgctggcgc acggatttca gtacccccaa
     7441 cgacggcgtt aagcccccct cttttggggc tgcgcataat tcaactctgt ctgcgccgtt
     7501 ttgctgcgcc gttagtgcac attccatgct gtaacagcaa atttccagta atgccatttt
     7561 tgctccttaa ttacgctgac tgctcgctgg caacgatctc ttcaatggac cacggatgaa
     7621 acttaatagt tgttttgcca tcggtgaccg ccagcgtcgg attcggtaat cgctcatgct
     7681 caccttttgg tgaactggtt ttaactgaga tccccggtag gctgagtcct tcatcagaaa
     7741 gcaatgccag cgcacgggca ttcagcgttt ctggatcacc cggcagaacg atttcaatat
     7801 gttcccaacc ttcgtgtggg taacgttttt ccccgggcca cggtagctcc acattggaaa
     7861 actgccagtg cgcaacctgt accggttcat gcaatttaaa cagacaaatc ggtctgccat
     7921 tgatcatttt ttctgacaaa agctcgccac actgttcaaa accgcgacgc cagcgctcag
     7981 cggtgacgtt ttgatggcaa cgcaaagaaa tgtgatcggc agtcagcgga gtgatatcca
     8041 accccagacg gcgggaaagt tcatctaatg cgtgggtaaa tcgcggtaaa tccgatgcaa
     8101 tatcctgcag ctcgtcgata gattgccagt tcgccataat cactcttcgt ctttcagtaa
     8161 aagcgttaat ttaccctgtt gccctgtgcc aaccaaccgc tgatttcacg ccgcttctga
     8221 tgcaatagtg aaaacggcaa tacgccacgc gcacgttgct gacgaaaaca gccatttgca
     8281 gtatactccc gccctaattt ctttaactgg tgcgggcaat ttttgctcgc ttcatcaatg
     8341 taaggtattc cggtgaatat tcaggctctt ctctcagaaa aagtccgtca ggccatgatt
     8401 gcggcaggcg cgcctgcgga ttgcgaaccg cagattcgtc agtcagcaaa agttcagttc
     8461 ggcgactatc aggctaacgg catgatggca gttgctaaaa aactgggtat ggcaccgcga
     8521 caattagcag agcaggtgct gactcatctg gatcttaacg gtatcgccag caaagttgag
     8581 atcgccggtc caggctttat caacattttc cttgatccgg cattcctggc tgaacatgtt
     8641 cagcaggcgc tggcgtccga tcgtctcggt gttgctacgc cagaaaaaca gaccattgtg
     8701 gttgactact ctgcgccaaa cgtggcgaaa gagatgcacg tcggtcacct gcgctctacc
     8761 attattggtg acacagcagt gcgtactctg gagttcctcg gtcacaaagt gattcgcgca
     8821 aaccacgtcg gcgactgggg cactcagttc ggtatgctga ttgcatggct ggaaaagcag
     8881 cagcaggaaa acgccggtga aatggagctg gctgaccttg aaggtttcta ccgcgatgcg
     8941 aaaaagcatt acgacgaaga tgaagagttt gccgagcgtg cacgtaacta cgtggtaaaa
     9001 ctgcaaagcg gtgacgaata tttccgcgag atgtggcgca aactggtcga catcaccatg
     9061 acgcagaacc agatcactta cgatcgtctc aacgtgacgc tgacccgtga tgacgtgatg
     9121 ggcgaaagcc tctacaaccc aatgctgcca ggaattgtgg cggatctcaa agccaaaggt
     9181 ctggcagtag aaagcgaagg ggcgaccgtc gtattccttg atgagtttaa aaacaaggaa
     9241 ggcgaaccga tgggcgtgat cattcagaag aaagatggcg gctatctcta caccaccact
     9301 gatatcgcct gtgcgaaata tcgttatgaa acactgcatg ccgatcgcgt gctgtattac
     9361 atcgactccc gtcagcatca acacctgatg caggcatggg cgatcgtccg taaagcaggc
     9421 tatgtaccgg aatccgtacc gctggaacac cacatgttcg gcatgatgct gggtaaagac
     9481 ggcaaaccgt tcaaaacccg cgcgggtggt accgtgaaac tggcagacct gctggatgaa
     9541 gccctggagc gtgcacgtcg tctggtggca gaaaagaacc cggatatgcc agctgacgag
     9601 ctggaaaaac tggctaacgc ggttggtatt ggtgcggtga aatatgcgga tctctccaaa
     9661 aaccgcacca cggactacat cttcgactgg gacaacatgc tggcgtttga gggtaatacc
     9721 gcgccataca tgcagtatgc ttacacgcgt gtattgtccg tgttccgtaa agcagaaatt
     9781 aacgaagagc aactggctgc agctccggta atcatccgtg aagatcgtga agcgcaactg
     9841 gcagctcgcc tgctgcagtt tgaagaaacc ctcaccgtgg ttgcccgtga aggcacgccg
     9901 catgtaatgt gtgcttacct gtacgatctg gccggtctgt tctctggctt ctacgagcac
     9961 tgcccgatcc tcagcgcaga aaacgaagaa gtgcgtaaca gccgtctgaa actggcacaa
    10021 ctgacagcga agacgctgaa gctgggtctg gatacgctgg gtattgagac tgtagagcgt
    10081 atgtaatcga tttttcgtga gagtgaagcc tgatcagggt tagccgatca ggctttttta
    10141 ttgccatcta aatgtattct gaaaacggac atgccattgt tttctcactg ttgagtaaga
    10201 ggccagaagc gtaatatcca ggcccaggaa aacgataacg gttcaattta aggaataccg
    10261 aagtgtttaa atttcttgta ttaacattag gcattatctc ttgccaggct tacgcagaag
    10321 atacggttat agtaaacgac catgacactt cagccatcaa agattgttgg caaaaaaatt
    10381 cagatgatga tactgacatt aacgtgatca aatcatgcct gcgacaagaa tacaatctcg
    10441 tcgatgcgca attaaataaa gcctatggtg aagcttatcg ttatatagaa caagtgccac
    10501 gcacaggtgc aaaaaaactt gatactgaac aacttaactt gcttaaaaaa tcacagcgag
    10561 cctggctgga ttttagggac aaagaatgtg aattaatcct ttcaaatgag gatgttcagg
    10621 atttaagtga cccctattct gaatcagaat ggctatcatg tatgatcata cagaccaata
    10681 cgcgaactcg ccagttgcag ctataccgta actctgaaga tttttatcca agccctttga
    10741 caagaggata attcacatct ttttggcatg ttttgttgca agctattcct gataaatgat
    10801 tgcaacaaaa catccagcct tttgactgag ttattaaaca tactcgcgag cgcgtaattt
    10861 tttgtctttc agcgataatt cacaatcact tcattccgtt gtacctttag cggtggaatt
    10921 aaccgccctg aaccgccccg ggtttcctgg agagtgtttt atctgtgaac tcaggctgcc
    10981 agatcatcgt ttccgatgga agcataataa gctttttctg cttctgccgg aggagt
//
LOCUS       DAEEUE010000118        10952 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.118, whole genome shotgun sequence.
ACCESSION   DAEEUE010000118 DAEEUE010000000
VERSION     DAEEUE010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10952
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.118"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..890)
                     /locus_tag="KLK84_003442"
     CDS             complement(<1..890)
                     /locus_tag="KLK84_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE2010639.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAP"
     gene            1131..1931
                     /locus_tag="KLK84_003443"
     CDS             1131..1931
                     /locus_tag="KLK84_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBE2010640.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2009..2779
                     /locus_tag="KLK84_003444"
     CDS             2009..2779
                     /locus_tag="KLK84_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBE2010641.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2827..4185)
                     /gene="mltD"
                     /locus_tag="KLK84_003445"
     CDS             complement(2827..4185)
                     /gene="mltD"
                     /locus_tag="KLK84_003445"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HBE2010642.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4257..5012)
                     /gene="gloB"
                     /locus_tag="KLK84_003446"
     CDS             complement(4257..5012)
                     /gene="gloB"
                     /locus_tag="KLK84_003446"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HBE2010643.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5046..5768
                     /locus_tag="KLK84_003447"
     CDS             5046..5768
                     /locus_tag="KLK84_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBE2010644.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5765..6232)
                     /gene="rnhA"
                     /locus_tag="KLK84_003448"
     CDS             complement(5765..6232)
                     /gene="rnhA"
                     /locus_tag="KLK84_003448"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HBE2010645.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6297..7028
                     /gene="dnaQ"
                     /locus_tag="KLK84_003449"
     CDS             6297..7028
                     /gene="dnaQ"
                     /locus_tag="KLK84_003449"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HBE2010646.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAVF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7161..7237
                     /locus_tag="KLK84_003450"
     tRNA            7161..7237
                     /locus_tag="KLK84_003450"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7195..7197,aa:Asp,seq:gtc)
     gene            complement(7368..8414)
                     /locus_tag="KLK84_003451"
     CDS             complement(7368..8414)
                     /locus_tag="KLK84_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HBE2010647.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8425..9410)
                     /gene="tssG"
                     /locus_tag="KLK84_003452"
                     /pseudo
     CDS             complement(8425..9410)
                     /gene="tssG"
                     /locus_tag="KLK84_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(9407..>10952)
                     /gene="tssF"
                     /locus_tag="KLK84_003453"
     CDS             complement(9407..>10952)
                     /gene="tssF"
                     /locus_tag="KLK84_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HBE2010648.1"
                     /translation="TAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPA
                     LHRYLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSA
                     IATLAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERF
                     YFFTPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVT
                     ARIDVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGN
                     HGRYFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVT
                     NRDLPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPLAEREMAWRLIRQLSFNYL
                     PLADLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGR
                     GVSCELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRA
                     GQRGSV"
BASE COUNT         2552 a         2848 c         2875 g         2677 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattctcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aattgttccg ctgcccggct aaagctgccg
      841 ctttcgacga ccgaaacaaa aatggcgagt tcttccgacg tggctttcat ttttgctcct
      901 gttgcaaaat agatgagata ttttgaattt atttgtcatt aaaccatcag gatgcgtgat
      961 atgtcatccg atttaatgtt ctcaataatg agcaaatttc tgacaggtgt aagcacttgc
     1021 ttacataaca atatacaatt gctcgttgaa agagtgagct aaaatcccta taacagtaga
     1081 accctcccga gtgcggaagg gttgacgtaa tagaggtttc aaagtcaaaa gtgcgaaaaa
     1141 acacctatgc catgcgctat gttgccggac aacctgcgga aaggatctta ccgccggggt
     1201 cttttgcgag catcggccag gcattaccac ctggagaacc gttaagtacc gaagagcgta
     1261 ttcggatcct ggtgtggaac atatacaaac agcaacgcgc tgaatggttg tcggtattaa
     1321 agaactacgg caaagatgca catctggtgt tattgcagga agcgcagaca acgccagagt
     1381 tagtacagtt tgcgaccgct aactatcttg ccgccgatca ggtgcccgct ttcgtgctgc
     1441 cacaacatcc ttccggtgta atgacccttt cggcggcgca tccagtgtat tgctgcccgt
     1501 tacgtgaacg agaacccatt ttgcgtctgg cgaagtcggc actggtgacg gtctatccat
     1561 tacctgacac ccgcctgttg atggtggtta atatacacgc cgtcaacttc agtctgggcg
     1621 tggatgtcta tagtaagcag ttacttccta ttggtgatca gatagctcac cacagcggcc
     1681 cggtcattat ggcgggagat ttcaatgcct ggagccgtag aaggatgaac gcgttatatc
     1741 gctttgcgcg ggaaatgtcg ctgcgccagg tgcgttttac cgatgatcag cgccgtcggg
     1801 cgtttggccg cccgctcgat tttgttttct accgtggtct gaacgtcagc gaagcttctg
     1861 tattggttac gcgcgcttcc gatcacaatc cgctactcgt tgaattcagt cccggcaagc
     1921 ctgataaata aggtatgtca ggtctgccac agggcagacc aacgtttggc gctgtccttt
     1981 tatactcaaa caacaaaagg acagcgcaat gacaacacaa tcccaccatg accacgtaga
     2041 aaagcagttt agctctcaag cctgcgaata tctgaccagt accgtacatg catccggacg
     2101 agatttacag cgcctggcgg tgcgtctggc tgattatcct ggtgcaagtg cacttgatat
     2161 gggctgcgga gcagggcatg ccagctttgt tgctgcgcaa aacgtgagcg cggtggtggc
     2221 gtatgactta tctgcccaaa tgctggatgt cgtggcacaa gctgccgaag cccggcaact
     2281 gaaaaatatc accacccgcc agggatatgc cgaaagtctg ccatttgccg ataacgcatt
     2341 tgatattgtt atcagccgtt attctgccca tcactggcat gatgttggtg cagcactgcg
     2401 tgaagtgaac cggatattga aacctggcgg taggatgatt gtgatggacg taatgtctcc
     2461 gggtcatcca gtgcgcgaca tctggttaca gacggtagaa gcattacgcg atacctctca
     2521 cgtacgaaac tacgccagcg gtgagtggtt gaggttaatc aatgaagcca atctgatagt
     2581 tgataattta attacagata agttaccgct ggaattttgt tcatgggtcg cgagaatgcg
     2641 tacgccagaa gcgttagtag acgctattcg catttaccaa cagagcgcat cgacagaggt
     2701 gagaacgtat tttgccttgc agaatgacgg ctctttcacc agtgatatca tcatggtaga
     2761 agcacataaa gcggcataaa taaaaaaggc accgggggaa tcggtacctt tttattatct
     2821 ggtttgtcag gaatctggca tgttgttgtt tttcacaaac aacgtcagct tgtcgcctgg
     2881 ttgcagattc gcagtgtcgc tgttccagcg catcacatct ttgatgttca cgccgtggcg
     2941 tttagcaatg cttgaaagcg aatcgccttt gcgcacacga tacgtaatgc tatcgctgtt
     3001 gtttgccagt cgctgtgcgc tactgcctgc accaatcgtc aaactttggc ctggcttcag
     3061 cttagatccg cgcagtttgt tccactgctg caaatctttg gtgcttacgc cgagacgtga
     3121 agcgatgctt gaaagcgtgt cgccagagcg tacggtgtaa acacggctgt taagcggcgt
     3181 attgttggca accagcgtcg actgtacagc agcaatttcg cctgaagcga gagattcacg
     3241 cagttgatct gcatgcttct ttggcaccat cacgtactgc ggcccacttg cgcccagcgt
     3301 ggagcctttc acgccagcgt tgaatgtctt cagcttgctg acggaaatcc ccgccatatc
     3361 tgcaaccttc gccatttcaa ccgggctact caggtgcaca cgcgccagag cacggctttc
     3421 atcggtcgtt ggcagacgta cgccataacg cttgctgttt ttgagaatat cactcaatgc
     3481 cagcatttta ggcacgtact gcttcgtttc ctgcggcaac ggtaacgacc agaagtccgt
     3541 ggatttccca cgcgctttgt tcgttttaat tgccttcatg acccgacctt cgccgctgtt
     3601 ataagccgct acggtcagaa gccagtcgtc gtcaaacatt ttgttcagac gctgcatcat
     3661 attcagcgcg gcagttgttg aagcaacaac atcgcgacgc gcgtcataat tgcgggtctg
     3721 tttcaaacca taattgcgcc ccgtgctcgg aatgatctgc cagatgcctg cggcattggc
     3781 gccagacgtt gcgtgaggat caaaagcgct ctccactatg ggtagtagta ccagttccat
     3841 aggcatgtta cgttttttaa cttgccctgc tatccagtac atatacggct ctgcccgtaa
     3901 agttacatcg tggagatagc tcttattgcg taaatatttc tgtttctgtt cgcgaatccg
     3961 gtcattttcc ggaattccca tctttagctc gtcgccaatg aaagcccaca agtcaccatc
     4021 tggcgcgata gacgtcccat cgtccatcca tcgtgcctga cttgtaaact ttgctgcttc
     4081 cccttgacca gctgcagaaa ggctctgtgc gtgctgttga acgttgccgg tactctggca
     4141 acccacgagc aggacagagg cgagtaatat cgcttttgcc ttcatgtgtg tgtcaatagt
     4201 tgcttaaaag acgaccgatc ataacggcga acggagccga tgacaagaaa gttttatcag
     4261 aacctatctt tctttgacct taaccatgca aaacgctctt caggttgttg caataatgtt
     4321 tcttcattaa ttccattaat taaatcaata tcttccgttc ttaaaaaaac attaatttgc
     4381 cgctcatttt tcagaattac gggtagtgtt atttgatttt ttgcccgtaa ctccttaact
     4441 ttacgataat aatcatttat ggacaaatcg tgcggaaaaa tactcaaagc aaacttcata
     4501 tttgataacg tatattcatg agcacaacat accaatgtat cgtcaggtaa cgcacttagc
     4561 tttttaagtg attgatacat ttgtaaagct gtcccttcaa acaaccgacc acacccacca
     4621 gaaaacagcg tgtcgccgca aaatagataa ggtttactga agtaacagat atgtcctaaa
     4681 gtgtgacccg gtgtggtaat tacactaaat tcatgcccta aaacgaaggc agtttcgcca
     4741 tctttgacta cctgtgttgt tcccttatct tgtgtctctt gtggaccata caccacaatt
     4801 tgcggaaact tttccaccag ttcttttacg ccgccaacgt gatcgtggtg atggtgggtt
     4861 agaaatatgg cctccggttg ccagttattg gcggtaatgg cgtttaatac tggctctgcg
     4921 tctccgggat cgacaatcag gcagcgacct gcctcatcat tcaaaaccca gatgtaattg
     4981 tcatcaaagg cgggaatact gttaagattc atagattacc tctcagtgta aaacggaagg
     5041 ttgtgatgaa accggcaaga gtccctcaaa ctgtcgtggc tcctgattgc tggggcgatt
     5101 tgccctgggg agagctttat cgcaaggcgc tggagcgcca gctcaacccg tggttcacta
     5161 aaatgtatgg ttttcatctg cttaagattg gcaatttaag cgcagaaatc aattgcgaag
     5221 cgtgcgcggt ttctcatcaa gtgaatgttt ctgcgcaagg aatgcccgtc caggtgcagg
     5281 cggatccact tcatcttcct tttgccgata aatccgttga tatttgtcta ctggcacata
     5341 ccttgccgtg gtgcaccgat ccgcatcgtt tattacgtga agccgatcgg gtactgattg
     5401 atgatggctg gctggtgatt agtggcttca accccatcag tttaatggga ttacgcaaac
     5461 ttgtgccggt attgcgcaaa acctcgccct ataatagccg gatgtttact ctgatgcggc
     5521 aactggactg gctctctttg ttgaattttg aagtgctgca cgccagccgt ttccacgttc
     5581 tcccgtggaa caaacacgga ggaaaactat tgaatgcgca tattcctgcg cttggttgct
     5641 tacaacttat tgttgcccgg aaacggacta ttcctttaac gctaaatccg atgaaacaga
     5701 gtaaaaacaa gccacgaatt cgccaggcgg ttggagccac ccggcaatgt cgtaaaccac
     5761 aggcttaaac ttcgacttgg tagcccgtat cttccagtgt gggattcatc gccgcggcac
     5821 gagccagttc atcacagcgt tcgttttccg ggtgtccggc atggccttta acccattccc
     5881 atttgatttg atgctgcccc aatgcagcat caagacgttg ccagagatcg acatttttta
     5941 ctggtttttt gtctgctgtt ttccagccac gttttttcca gttatggatc cactgggtga
     6001 taccctggcg gacatactgg ctgtcggtac tcaaaatgac ttcgcaatgt tcttttaacg
     6061 cctccagcgc gacaatagcg gccatcaact ccatacggtt gttggtggtg cgggtgtagc
     6121 ctgcgctaaa ggttttctcg cgtccgcgat agcgtaaaat agcgccgtaa cccccaggtc
     6181 caggattacc cagacacgaa ccatcggtga aaatttctac ctgtttaagc atctctggta
     6241 gacttcctgt aattgaatcg aactgtaaaa cgacaagtct gacataaatg accgctatga
     6301 gcactgcaat tacacgccag atcgttctcg ataccgaaac caccggtatg aaccagattg
     6361 gtgcacacta tgaaggccac aagatcattg agattggtgc tgttgaagta gtgaaccgtc
     6421 gcctgacggg caataacttc catgtttatc tcaaacccga tcggctggtg gatccggaag
     6481 cctttggcgt acatggtatt gctgatgaat ttttgctcga taagcccacg tttgccgaag
     6541 tagccgatga gtttatggac tatatccgcg gcgcggagtt ggtgatccat aacgcagtgt
     6601 tcgatatagg ctttatggac tacgagtttt cgttgcttaa gcgcgatatt ccgaagacca
     6661 ataccttctg taaggtcacc gatagccttg cggtggcgag gaaaatgttt cccggtaaac
     6721 gcaacagcct cgatgcgcta tgtgcccgct acgaaataga taacagtaaa cgaacgctgc
     6781 acggggcatt actcgatgcc cagatccttg cggaagttta tctggcgatg accggtggtc
     6841 aaacgtcgat ggcttttgcg atggaaggag agacacagca gcaacaaggt gaagcaacaa
     6901 ttcagcgcat tgtacgtcag gcaagtaagt tacgcgttgt ttttgcgaca gatgaagagc
     6961 tggcagcaca tgaagcccgt ctcgatctgg tgcagaagaa aggcggaagc tgcctctggc
     7021 gggcataaat acatgtaaaa ggcgctaaaa atagcgactt gggcgatttt tgcagcaaac
     7081 gattcaaaag atgagaaaaa tcgttgacga aggtcgaggc aatccgtaat attcgcctcg
     7141 ttcccaacgg aacacaacgc ggagcggtag ttcagtcggt tagaatacct gcctgtcacg
     7201 cagggggtcg cgggttcgag tcccgtccgt tccgccaata ttcagaaagc ccgaatcagt
     7261 aatgattcgg gctttcgtcg ttttggtgaa gtgagaattt ttcactccga agtaaaacgg
     7321 atatgattta aaaaatggcc agacattata aattctggcc acttctgtta ctccagcgca
     7381 tcccatttct ccagcagttc tactggaatt tcatttatta cctccgagac gcgttttccg
     7441 accaggcgtt ctgcctggcg cagcaaagga atggtcgggc tgctcggttc gctgtgctca
     7501 aaccagcgac ggatgacacg caggcgttcc agcgcgtcgt ttcggtcgcg gatctgccgg
     7561 ggttctgcgc cggtcatcgg tgatatttcc tccgcgatat gcaccggtag cgcaatctgc
     7621 tctgcctgca tctgttcagt atgaacgatg gtggcgtttt ccggctgctc agcctgtggt
     7681 tgtggcagga tctccggcgt ggtgctctgc actgctcccg gtaacagggc cagcagttgc
     7741 gtcagacggg agaagtccgg tgcctggtca ttcagcgttt cacgcgcatg gcgttgcagc
     7801 cgctcagtgg tctctgctgc ctggcgaaag gcatccagcg gcagtgtgcc gcgcgcttcc
     7861 aggtctgcca gttgctggcg taccgattct ggtgccagcg catccgccgg gcgtaatgca
     7921 gatagcgaac gctccacatc ccgcacctga aggcgcatgg cagcattatt tgacagtgta
     7981 atgccacgaa tatccgccat tacaccttcg tggtccagca acgccgccag cgcgttactg
     8041 cgtgccaccg cagcatcttc ggcggtgata tcttcagtcg cgagcagttg aggatggatg
     8101 gcgtccgggt acgtggagca caacgtttcc agcagcgtga gcgcctccga cagtcccctg
     8161 gcaccggcct gctggatacg gcagcgcagc aggacaacca gaatgcggat atctttgctg
     8221 cgcatcagca gacgatgggc gtcccgctca agctccgccc agttaatgtt ttccggcgta
     8281 ctgacaaaat cgccatattg cgcttcttca cgcggtgcgg cgcgggtgaa cagcagcagg
     8341 tattccggat cgtactccag atccgggccg caggggcatt ccgccgaaat tgggtcagac
     8401 aggaatattt ccatttaatt atcctcagtg cgccaggtac tgttctggtt cgaaaatcat
     8461 gcccgtcacc gggttgtcgt gcatggcttt acccgcccat gcagtccaac ccagttggta
     8521 cggaccaccg gtaacagccg gaggggcggc gtggggcttt agctgtagct caatttccca
     8581 gcagtattca taaccactaa aggcgcggac cagttctgtc aggacgggca ggttattgct
     8641 gccaggcaga aaacgtcggt aatcctccag cgtcagcggg ccgataacca gccggaactt
     8701 atactgcatg tccggcacgg cctggccgat gagtgcacca ttgccaataa cggaagaggg
     8761 ggcaggcatc cccagccggg tgatctcatc gtccgccacg gctatccagt gcaggacata
     8821 ctcctgtatc cggaaaggta caccgaaata gtgcgccagc gtggtggcca gaccgtccgg
     8881 attgcgggac tcacgcacca gatgggccga tgccgccaga cgagcgtgat ctggcaacgg
     8941 gctgtcagcg ctctcccgca aatcctgccc gctgaggctg gcgacataaa aggcaaagcg
     9001 gtcatgttgc ggtttatcca gaccgccccc agcggactgg gcgctgcgcc atgcctggta
     9061 aaactgcgtc agccagcggt gatgaaaaag atcagaaaaa cgggtcagcg tcggctttca
     9121 gtccggttac gcgccagttc ggtgtagtgc agcggcagtg gaccgttcgg cccccacagc
     9181 ccgaggctgt acagactgat gtgcaggcgc ccttcctgcc agcccacact ggcgatttct
     9241 cgtggcgcaa aggtcagcgc gggcgtttgt ccgaggcgaa atttttccat ccgtggttgc
     9301 caggtgttgc ccgccggaac ggtacacaac tgcacatcca cgcggcgcat caggttcaga
     9361 aaaccgtaac gccagggggg ggcctgcgcc tgagtgaaat cgttgctcat acgcttcccc
     9421 gctgtcccgc cctgacaggc caggtcataa ttttgccgcg ctgcatgctg tgcagcgtca
     9481 tctgcgagaa ggtattaatg gaaacatggc gggcgatata gtgttccagc accaggccga
     9541 acagatacgg actgatgccg gaaaaacctt cttcatccac cgtcagttca caactgacgc
     9601 cacgcccata gaccagtagt ccggaaccgg gcagacggcg ggtgaccggg gtcgttttac
     9661 aaccaatcag gctgcgcacc tggcgcgatt gcggactgtc atgagcagga ataaacaggt
     9721 tcagcagatc tcgcaacgcc tggccgccgg tacggtgatc cagatcggcc agcggcagat
     9781 aattaaaaga taactggcgg atcaaccgcc atgccatctc acgttcagcc agcgggggct
     9841 gcggcgagcg tggcgggcgg ataagcccta cgcccgccac cgggatggct gcatccacgg
     9901 ttagatcgtc ccggccattg cgggcaataa gacagggcag atcgcggtta gtgaccatgg
     9961 ccgtgatggt gatgtggcgc agattttccg ggtacggcgc ttcatactgg tcaaccagcg
    10021 acaggaacac ttccgagccc gtataggggg tacgggtgcc gtaacggcgg gcgttttctg
    10081 ataagcggcg gggctcccgg cgcagggaaa aataacgtcc gtgattgcct tcatcattat
    10141 tgcgggtgtg gtagagcggg cgaaaggcca tcttccgtgt ggtgtcggtt tccagccctt
    10201 ccacttcctg gaccgagaac acctcataat ccagcggatg ggttctgtcc accaccagat
    10261 gctgttccgt cgtgctgtgc gtgacatcga tccgggcggt gacgcgggga aacaggttga
    10321 taaccggggt acagaacagg ctgaactgtg ccgcatttgt ctggtggatc agccagtccg
    10381 gcggcaggcg gttcagcagg ataacaatct ccgccacgcc accgtcaatt ttctgtaatc
    10441 cggcagacag gcctgtcggg gtgaaaaaat agaagcgttc cggacaggca aaatattcat
    10501 gcagcaggtt atgaccgtga aacacgttcc acgccagcgg cagcagcccc tgatccggct
    10561 ccaggccttc gtacataacg ggctgttgca gattcacatc cagcgcgccg tcaaaatgcc
    10621 ccggaatgcc agccagggtc gcgatggcgc tggtatgtag aagttctaac aggtgtgagg
    10681 ccgtacgctc ctcgccgcag agataaaacg gcagcctgtc cagtccggca agctgactga
    10741 acgttagttc accaaaggtg cgcagggtaa tacgcagcgc cccggcgacg tgaatgttgg
    10801 ccggcaggta gcggtgcagg gcaggcatat ccggtggcgc ggcggtcagt cgcgcctctt
    10861 cgatggcgag cggccacagc gttacatcct ggctgctgcg aaactggcag gcagtgcttt
    10921 cgccttccgg aatggcggag aaaaaggctg tg
//
LOCUS       DAEEUE010000119        10901 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.119, whole genome shotgun sequence.
ACCESSION   DAEEUE010000119 DAEEUE010000000
VERSION     DAEEUE010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10901
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.119"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(152..874)
                     /gene="baeR"
                     /locus_tag="KLK84_003454"
     CDS             complement(152..874)
                     /gene="baeR"
                     /locus_tag="KLK84_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HBE2010649.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(871..2274)
                     /gene="baeS"
                     /locus_tag="KLK84_003455"
     CDS             complement(871..2274)
                     /gene="baeS"
                     /locus_tag="KLK84_003455"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HBE2010650.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(2271..3686)
                     /locus_tag="KLK84_003456"
     CDS             complement(2271..3686)
                     /locus_tag="KLK84_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE2010651.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(3687..6752)
                     /gene="mdtC"
                     /locus_tag="KLK84_003457"
     CDS             complement(3687..6752)
                     /gene="mdtC"
                     /locus_tag="KLK84_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HBE2010652.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(6753..9875)
                     /gene="mdtB"
                     /locus_tag="KLK84_003458"
                     /pseudo
     CDS             complement(6753..9875)
                     /gene="mdtB"
                     /locus_tag="KLK84_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            complement(9875..>10901)
                     /gene="mdtA"
                     /locus_tag="KLK84_003459"
     CDS             complement(9875..>10901)
                     /gene="mdtA"
                     /locus_tag="KLK84_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HBE2010653.1"
                     /translation="YLTGLGTITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEI
                     DPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSRQELDAQQALVSETE
                     GTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPI
                     DLVFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKV
                     KARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSENKVSKH
                     LVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQSATTPEEKATSREY
                     AKKGARS"
BASE COUNT         2510 a         3070 c         3003 g         2318 t
ORIGIN      
        1 ctaaaggtgc tgtttactga ttaaagtatg agcgattttc tgatgcctgc cagggcggag
       61 agataatgtc agtttgctga ccatgcgtgg aataatgttg ccggatcggg tctgaacgcc
      121 atctccggct aacaaagtaa tgtcgctaaa actaaacgat gcggcaggcg tcggcttccc
      181 agcggtaacc aacgccgtaa acggcgcgga taaatgactg ttcggcgtcg agagattcca
      241 gcttgcggcg cagattttta atgtggctgt caatggtgcg gtcggttact acgcggtagt
      301 cgtcataaag atgattgagc aattgctcgc gggagaacac ttttcctggt tcgtgagaga
      361 gcgttttaag cagacgaaat tccgcaggcg tcaggtcaag cattttaccg cgccatgaag
      421 cctgaaaacg accttcgtcg ataatcaacg ggctttcggc atcctgttgc tgaagttcgc
      481 gttgcggttt acaacggcgc aaaatggttt tgacacgcgc taccacttcc cgtgggctgt
      541 acggcttgca gatgtagtca tcggcgccaa tctccagccc cagcaggcgg tcgatctctt
      601 cgatttttgc cgtcaccatc acgatcggaa catcggaaaa acgacgaatt tcccggcata
      661 atgtcaggcc atcggtgcca gggagcatca gatccaacag gatcagatcc ggtggtgtct
      721 ggcgcacata cgccagtacc tgatcgccgt ggctgataag cgtcggcgca tagctcgcag
      781 cacgcagata atcaatgagc aactgcccca gcttcggttc atcttccacg atcaaaatac
      841 gcggtgtgtt ttcgtcgatt ggtaactcgg tcatacttct ctctgtaaat cccgttccag
      901 cggtaactct actgtaatgc ttaccccgcc aaaaggcgaa tgggcggcaa taatgcggcc
      961 attatgggct tcaacaatgt tcaggcaaat cgccagcccc agcccggaac cgccgctggc
     1021 gcggttgcgg gaaccttcgg tgcgataaaa acgttcaaac aatttttgta gctgatcgtc
     1081 actgacacct ggcgcactgt cggcaaaggt caggcgcacc gttttgtcgt gctgctcggc
     1141 agagattttc aggctgccgc cgctgtcagt gtagcgcagg ctgttttcca gtaagttatt
     1201 gaataactgc attaaacggt cgcgatcgcc aaatacggtg atactgtctg gcagggaaaa
     1261 ttgcagtttc aggccacggc tggcgaatcg ttcgcgaaat gcgccgcccg ccacttccag
     1321 cagtgggatc aaatctaccg gtgctttttg ataggcgaga gcgccttcat cagacatcga
     1381 caactgatga agatcgtcaa ccagtttggt cagtgtaccg acctccgcct gtaaagacgc
     1441 caccgtctcc ggcgtgaatt tgcgcacgcc atcctgaatg gcttccagtt caccgcgcag
     1501 caccgctaat ggcgtacgca gttcgtgaga aatatcggcc ataaaatcgc gccgcatttg
     1561 ctggtttttc tccagcgtgc tggcgagctg gttgaagtct tgcgccagtt tgcccagttc
     1621 atcttcactg gtgggcgtta cgcgggtagt gaaatcgccc gccgccagtt tgtgcgtgcc
     1681 atcgacaagt cgttttaccg gtgccagcaa accgcgcgcc agcagaaagg tggcgagcgc
     1741 cgcgagtaac gttgccaggg cgacgatcag ccagctggtt tgccgctgtt gtttatcgaa
     1801 attgatatca gtattgcggg tcagccgctc aacaggggag gcaatcaccg cgcccacttc
     1861 cgcaccgttg accagaatgg gtcgccgtgt accgtcaggt ggaatcggcg ctcgcggacc
     1921 aaccagtact ttgttgtttt gatcgaccac ccagaactgg gtacgccagc cgtgcggcgg
     1981 catacccggg ccgggtttat cttccgaatt atcgtgttca aatgaacgca ggatctgaaa
     2041 gacaaagcga tcgttgttgc gcaggaagcg ccagttacca tgctgcgcat actgctcgcc
     2101 aagcgcatca cttaacagct gtaatcgctg ttcattgccg tgtttgatgt aatcaataaa
     2161 gccacgctca aaactgatac gcaccgccca gtgcatactg atcagcaaga caatgcaggt
     2221 ggcgaaaatc gccagaaaca gtttgccggt aataccgggt cgccagaact tcattgcgcg
     2281 ctcctttttc gccgcgaaat agctacattt tgatgcgtat cgttcggcac tctggcaaag
     2341 atgaacgccg gaagggcgat gataaacgcc atgctaagcc aggtgtacat aaagacggtt
     2401 tgtgtggtgc cgctgtcgac gctgatatgc tgcgaaccaa aaagccccag caacagcccg
     2461 gcgatggtga cgccgatact catcgacaat tgcataatca tcgacagcag gctgtttccg
     2521 ctgctcgcca gattgtccgg gagatctttc agcgtcaggg tgttcatgga ggagaaacgg
     2581 gtcgagttga ccatcccttg taaaaacagg acgaacggca aaacgtagta ccagcccaac
     2641 agagcggtag tcataaacaa cagggtgacc agcgacagac ccagcgtggt cgctaccagt
     2701 acccgacgat aaccaaagcg attcaccacc tgtaccacaa ttcgcttcat tcccatgctg
     2761 ccaagcacca tcgggatcat catcagtccg gcatgaaacg gcgagaaacc gaggccaatt
     2821 tgcaggaaaa ccggtgtcat aaagggcaac atgccactgc caatacgtcc ggcaaagctc
     2881 cccgccaggc ccagcgaaaa ggtacgagtg cggaacagtt tcagactgaa cagggcgcgg
     2941 ttgttatttc tggcgtgcag cagataaagc accagtgcca ccacgccaac tgcgaccagg
     3001 ccagcaatcg ccagcggcga taaacctgta cctttactgc cgtccagcgc cagggttaat
     3061 accgccatgc caaccgccag caataaaaag ccggaaagat caaagcgccg cgtctgcatg
     3121 gtgtagttcg gcattaacat caatgtggcg atcgcaccga taatccccac cggaatgttg
     3181 atcaaaaaga tccagtgcca cgacgcgtat tctaccagca gaccgccgag cgccggaccg
     3241 agcagcggac cgacctgacc gggtaacgtg acaaaggtca tcgccgccat atattgctcg
     3301 cgcggtacga ttttcatcac cgtcaacctg ccgaccggca ccatcatcgc accgccaacg
     3361 ccctgtaacg cgcgtgccag caacagttcg ttcagcgtgc cggaaagcgc gcaaaacagt
     3421 gaaccgagag taaacagcac gatggcggta aagaaaatat tgcgcacgcc gactttgtcc
     3481 gccagccagc cgctggcggg cagcatcacc gccacggtca gcacataaga gacaatgacc
     3541 atgtgcatat gcaacggact ttccccgagg ctttgcgcca ttgagggaag ggcggtgttt
     3601 acgatggtgg tgtccagcga ctgcataaag aagccgaaag ccacaatcca taattgccag
     3661 cgggtgctgt cgggaagatc tgtcatttac tcggttaccg tttgtttagg tttacgcgaa
     3721 aaacgcagcc gcagacggtc gaaaaagaga tacaccaccg gcgtggtata cagcgtcagg
     3781 agctggctca tcaccagacc gccgacaatg gtgatctcca ggggttgccg cagctccgag
     3841 ccgtcaccgc ccgacaatac cagcggcagc gcgccaaaca gcgccgccag ggtggtcatc
     3901 ataatcgggc gaaaacgcag cagacaggcc tggaaaatag cttcctgcgg cgtcaggtta
     3961 ccgtgccgtt gggcttcaag cgcaaaatcg accatcataa tggcgttttt cttcacgatg
     4021 ccgattaata gcatgatccc tatcagggcg attaggctga acggggcatt gaacagctcc
     4081 agcgccaaca gcgctccaac gcccgccgag ggcagggtgg agagaatcgt cagcggatgt
     4141 acgtaactct cgtaaaggat acccagcacg atatacaccg tggcgatggc ggcgataatc
     4201 aggatcacct gcgagttcat cgtctcctgg aaaacctgcg ccgtgccggc aaaactgccg
     4261 cgcaccgtcg aaggcacacc aagctgggtc attgcgcgat cgatcgccgc actggcgtcc
     4321 gagagcgatt ttccggtcgg caggttgaac gagatggtcg aggccgccga taatccctga
     4381 tgattcaccg atagtggggc attcgccggt tgccatttag cgaaatacga cagcgggatc
     4441 gctttgcctt cgttattgat aacgaacatt ttttccagcg cactgatgtc ctgggtatag
     4501 cgcggatcca cttccatcac cactttatac tggttcatcg gctggtaaat ggtcgagatt
     4561 tgccgctgac cgaaggcgtt atttaacaga ctgttggcgg cctgtacgtc gattcccagc
     4621 cgtgccatgg tgtcgcggtc gtaaaccaga ttcatctccg cgccgttatc ctgctgatcg
     4681 gagttcacgt ccgccagttc cggcaacgtc gccagttttt tgcggatttt cggctcccat
     4741 tcgcgcagtg ccgccaggtc gtcggataac aacgtgtact ggtagctggc gttcgactga
     4801 cgcccaccaa cgcgaatatc ttgtaccgcc atcaggaaca gattcgcccc cggttctttc
     4861 gccagcttca cgcgcagacg gtcgataatt tgctgcgccg tttcgctgcg ttcgtcgcgt
     4921 ggcttgaggg tgataaacat catcccactg ttaactcgtg aaccgccggt aaagccggtg
     4981 acattatcca ctgccggatc gtcacggata attttcatga aatcctgcaa tttgccgcgc
     5041 atcgcctgaa acgaaatact ctgatccgcc tgtatcccac ccatcagtac gccggtgtcc
     5101 tgctccggga agaaggtttt cgggatcgag atattcagcg caatggtgcc aagcagcacc
     5161 acgcccacca gacgggtatg attgagcacc catttcagcg acttgccgta gccttgttgc
     5221 agggctacca acatgcggcc aaaaccacgc agtcgctttt gctcacgcgg cttgctggct
     5281 tttagcatcc agccgcacat cattggcgtt agcgtcaggg aaaccagcaa cgaaatacca
     5341 atggcgacag aaagcgtcac ggcaaactcg cgtaacagtc ggcccggcaa tccgcccatc
     5401 aacagcagcg gcaggaacac cgccaccagt gacagactca tcgacagcac cgtaaagccg
     5461 acttcgcgag taccttgcag cgcggcctgt agtggtttca tccccgcttc cagatgacgg
     5521 gcaatgtttt ccagcaccac aatggcgtca tccaccacga aaccagtagc aatggtgagc
     5581 gccatcagcg aaagattatt gagactgaat ccgcacaggt acatcgccgc aaacgtacca
     5641 atcagcgaaa ccggcaccgc aacggcggga ataatagtgg cgcgacccga gcgcaggaat
     5701 aaaaagacca ccagaatcac cagcgccacc gagataatca gcgtttgctc gacttcttcc
     5761 agcgaggcgc gaatggtggg ggagcgatcc tgggcaattt gcagatcaat cgccgccgga
     5821 atggtctcct gcaactctgg taattttgcc cggatgctgt ctaccgtctg gataatattg
     5881 gcttccggca gtttgcggat catcagtaaa atagccggtt tggcgttggt catcccggcg
     5941 ttgcgcacat cctgcactga gtcagtcacc gtcgccacat cgcccagacg aaccgcgccg
     6001 ccgttgttgt agtgaataat cagcggctga tattcagcgg cggttttcag ttcatcgttg
     6061 gtctggatct gccagcggtg agtgccatct tccagcgccc cctgcggttt acgcacgttg
     6121 gcattgctga tggcagtgcg tacgtcgtcc agcgacacgc cctgattaaa cagcgcctgc
     6181 ggggtcagcc cgacgcgcac ggcgggcagc gagctgccac cgacatcgac atcaccgaca
     6241 ccgtcgattt gcgaaatcgt cggagccagc tgcgtcgagg cgaaatcgta cagttcaccc
     6301 tgcgaataag tatcggatgt cagcgtgagg atcataattg gcgcatccga cgggttcgct
     6361 ttgcgatagg tcgggcggct gggcatcccg ctgggcagca aactttgtgc agcgttgatc
     6421 gccgcctgca catcacgcgc tgcgccgttg atatcccggt caaaatcaaa ctgcaaaata
     6481 atgcgcgtgc tgccgagcga actgctggag gtcatttcac taaccccggc aatgcgccca
     6541 agtgagcgct ccagcggcgt ggcaacggaa gacgccattg tctccggcga cgcaccgggc
     6601 agcgaggcgc tgaccataat caccggaaaa tcgacctgcg gcagcggggc gaccggcagc
     6661 atacggaaac ccagtatgcc gcacagggta atggcgaccg acagtaaaat cgtcgccacc
     6721 gggcggtaaa tgaagagggc aaaaaacttc acttacgcct cctcttcatg gcgagcaaag
     6781 cgacttttgg tccacaatgc caggcggtcg aacagcagat aaatcaccgg cgtggtgaac
     6841 agcgtcagca cctggctgac aatcagaccg ccgaccatgc cgatacctaa cggacgacgc
     6901 agttccgcgc cgaccccggt actcaacatc agcggcagcg cgccaagcag agccgccagg
     6961 gtggtcatca ggatcggacg aaaacgcaac agacaagcct ggtagattgc atcgcgcggc
     7021 gacatgcctt gctcgcgctc agcagccagc gcgaagtcga tcatcatgat ggcgttcttc
     7081 ttcacaatac cgatcagcaa aataatgccg ataatcgcaa tcacatccag ttcgctaccg
     7141 gcaatcatta atgccagcag cgcgccgacc cctgcggtgg gtagcgtcga gagaatggtg
     7201 atcgggtgaa taaagctctc gtacagaatg ccgagcacga tatacatcgc caccaccgcc
     7261 gcgacaatca gccagacagt gctgcccagc gccgactgga aggcgagggt gctgccctag
     7321 aattgcgtgg tgatatccac cggcagattc agcgtctttt cggtgtccat aatcgcctgc
     7381 accgcatcgc ccagcgaata gttatccggc acgttaaagg agatggttgt taccgggaac
     7441 tgatccagat ggttgatgga aagcggcgca aaacgctgct caattttggc aattgagctt
     7501 aacggcacca cgccaccgtc gctgctggtc aggcgaatgg tatccagcgc cgcgaggcct
     7561 ggggtatttt cggtattgtg ctccagcacc acgcgatact ggttagcctg agtataaata
     7621 gtggaaatca gtcgctgacc aaacgcgttg tacagggcgt tatcgacgtc cgccatgctg
     7681 atccccagac ggctggcgct gtcgcgatca acattgacat acgccaccag ccctttgtcc
     7741 tgccagtcgc tggagacatc agaaagctgt ggcagttgct ggagtttttc catcaactgt
     7801 ggcacccagg tactgagcgc atccagtgac gtggcctgca aggtaaactg gtactgggtg
     7861 cggctgacct gagtatcgat ggtcaggtcc tgcgttggtt gcaggaagag atcgacgccc
     7921 ggcactttat ctaccgccgt ttgcagacgg gcgatgactt tttgcacccg atcatcacgt
     7981 tcatccaacg gtttgaggtt gatttgtaaa cgtgcactat tcagcgacgg gttagtgcca
     8041 tcaacgccaa caaatgaggt caggctttgc actgccggat cctgcaaaat cacgtccgcg
     8101 acctggcgtt gtcgctgggc catattggca aaggagctgg attgcggtgc ctgcaaagtg
     8161 ccctgaataa tgccgttgtc ctgtaccggg aagaaacctt tcggaatgaa cacccacagc
     8221 agcacgctaa gcagcagcgt gctgagtgcc acgcttaagg tcagccacgg atgattcagc
     8281 actttcgcca gtccacgacc ataggcggcg attatcctgt cgaacatttt ttccgaggca
     8341 cgggagaagc ggttctgttt acgcaacgac tcctggctga gcatccgcgc gcacatcatc
     8401 ggtgtcaggg tcagcgacac caccgctgag atcaaaatcg ctaccgccag ggtaatagca
     8461 aattcgcgga acagtcgccc gacgatatcg cccataaaca gcagtgggat caacaccgca
     8521 atcagtgaga aggtcagcga gataatggta aagccgattt cacctgcgcc cttgagcgcc
     8581 gccgccaacg gtttttcgcc tttttcgata tagcgggaaa tgttttcgat caccacgatg
     8641 gcgtcatcga ccacgaatcc ggtggcgata gttaacgcca tcagtgtcag gttattgatt
     8701 gaaaaatcga gaaacaccat aaccgcgaaa gtgccgatta acgacagcgg tacagcaaca
     8761 ccgggaatga tggtcgccgg aatattgcgc aaaaacaggt agataatcat gactaccagc
     8821 gcgatagcca tcatcaattc aaactgagta tcatcgacgg atgcgcggat attggtggtg
     8881 cgatcggaaa gcactgtcac cttcaccgat ttcggcagac tctcagtgag ctgtggcagc
     8941 atctgccgaa tgctgtcggc ggtggagata atgttagcac cgggctggcg ctgaacattc
     9001 atcacaatgg cctgttcttt gttcgcccac gcgccgagcc agctgttttc tgcaccttgc
     9061 tctacagttg cgacatcgcc cagacgaatt ggcgcgccgt tctggtaggc gatgattagc
     9121 tggcgatact cttcggcgga ttgcatctgg tcgttcgcgg aaagcgtgac cgcacgggaa
     9181 gggccgtcga ggctaccttt tgccgagtta acgttagcgc cggtaatggc ggtgcgcacg
     9241 gtttcgctgg tcaggccgag ggcggcaatc gcctgagcgt taagtttgac gcgaacagcc
     9301 ggacgctgac cgccggaaag cgtcaccagg ccgacgccgg aaatctgcga gattttctgc
     9361 gcgacgcggg tttccaccat atcttccact tgcgtcatcg gcatggcggt tgaggtgacg
     9421 gcgagcgtca tgatcggcgg atctgccggg ttcactttgc tgtaaaccgg cgggttaggc
     9481 agatcgctcg gcaacaagtt ggtcgcagcg ttaatcgcgg cctgcacttc ctgctcggcg
     9541 acattgagcg gtaatgttag ctggaactgc aaagtgataa ctgacgcacc gccggaactt
     9601 tgcgacgaca tctgtttcag gccagacatc tgcccgaact ggcgttcgag cggcgcggta
     9661 acggcagagg tcatgacatc cgggctggca cctgggtaga gcgtgaccac ctgaatggtc
     9721 ggatagtcca cttccggcag cgccgaaacg ggcagggcgc gataaccgat aatcccggcg
     9781 agtaagatcg ccaccatcag cagcgtggtg gcgacaggac gcataataaa caggcgcgac
     9841 gggccgcctg tgctgctcgg gggtaacacc tgcatcagga gcgtgctcct tttttcgcgt
     9901 attcgcggct ggtggctttc tcttccggag tggtggcgct ctgggcttcc accacttcca
     9961 ctttcgcccc ttcggtcagg cgatcaatgc cgtctgtcac cacgcgatcg cccgcagaaa
    10021 tacctgcacg gatcaccact ttctgactgt cctgaatgcc cggcgtcacc agatgtttgc
    10081 tgaccttgtt ttcgctattc agcacccaga caaaatggcc ttcattgccc atttgcaggg
    10141 cggctgttgg gatcactacg gcgttttgtt cggtgtcgac taacatgcgc gcgttaacaa
    10201 actgattggg aaacagcgca tcatcctgat tattaaagcg tgctttcact ttaatcgtac
    10261 cggttgtggc atcgatttgg ttatcgaggc ttaacagcgt gccttcactt aatttcttcg
    10321 aattggtgcg atcccaggct tctaccatca gcggttttcc ggctttttgc gcctgcacta
    10381 cagtagcgat atcgctttcc ggcagggtaa agactaaatc gataggatgc gtctgggtga
    10441 tcaccacaat tccggtggta tcaccactgg agatttggtt accaacatca acttgcttga
    10501 gaccaacgcg accatcgact ggtgcggtga tgcggctcca gtcgagttgc aactgcgcgc
    10561 tggcgacgct tgcttcatca gccttaatgg tgccttcggt ttcactgacc agcgcctgtt
    10621 gggcatccag ctcctgacgg gagacgagat tggtttttgc cagttgctga taacgcgcca
    10681 gatcacggcg ggcgttagca agcgtggctt tatcttttgc cagttggccc tgggtttgtg
    10741 ctaatgcaac tttgaactgg ctggggtcaa tttctgccag taaatcgcct gctttgacct
    10801 gctggccttc ctggaaatgt aacgccatca gttgaccgtc cacgcggctg cgcaccgtaa
    10861 cagtattagc ggcggtaatg gtgccaagcc cggtgaggta a
//
LOCUS       DAEEUE010000120        10747 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.120, whole genome shotgun sequence.
ACCESSION   DAEEUE010000120 DAEEUE010000000
VERSION     DAEEUE010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10747
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.120"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(133..1482)
                     /gene="lysC"
                     /locus_tag="KLK84_003460"
     CDS             complement(133..1482)
                     /gene="lysC"
                     /locus_tag="KLK84_003460"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HBE2010654.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLELGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            2007..3656
                     /gene="pgi"
                     /locus_tag="KLK84_003461"
     CDS             2007..3656
                     /gene="pgi"
                     /locus_tag="KLK84_003461"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBE2010655.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            4118..4360
                     /locus_tag="KLK84_003462"
     CDS             4118..4360
                     /locus_tag="KLK84_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010656.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTITTTTTSTQ"
     gene            4474..5112
                     /locus_tag="KLK84_003463"
     CDS             4474..5112
                     /locus_tag="KLK84_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HBE2010657.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            5109..5846
                     /locus_tag="KLK84_003464"
     CDS             5109..5846
                     /locus_tag="KLK84_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HBE2010658.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            5846..7942
                     /locus_tag="KLK84_003465"
     CDS             5846..7942
                     /locus_tag="KLK84_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HBE2010659.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(7989..8267)
                     /locus_tag="KLK84_003466"
     CDS             complement(7989..8267)
                     /locus_tag="KLK84_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010660.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            8268..8411
                     /locus_tag="KLK84_003467"
     CDS             8268..8411
                     /locus_tag="KLK84_003467"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010661.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            8537..8947
                     /gene="psiE"
                     /locus_tag="KLK84_003468"
     CDS             8537..8947
                     /gene="psiE"
                     /locus_tag="KLK84_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HBE2010662.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(8991..10466)
                     /gene="xylE"
                     /locus_tag="KLK84_003469"
     CDS             complement(8991..10466)
                     /gene="xylE"
                     /locus_tag="KLK84_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HBE2010663.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
BASE COUNT         2727 a         2741 c         2778 g         2501 t
ORIGIN      
        1 gtttttctgc tcattattag ctccttctga tgccattcta tttcaggaag gagtgtccgt
       61 taaactcagg ctacctcata gtgacaagaa aatcaatacg gcccgaaata tagcttccag
      121 gccatacagt atttactcaa acaaattact atgcagtttt tgcaccacct gctcggcatc
      181 ttcgccgggc accaggaagc acaggttatg gctggatgcg ccgtaacaaa tcatgcgaat
      241 gttgaacggt tccagtacgc cgaatacctc tttgccaacg ccgcaggctt ttgacaggtc
      301 attgccaatc aacgcgacca gcgccagacc ttcttccacc tccacccggc acagtgcgga
      361 aagctccatc agcagagatt gcgtcagcaa cgtatcgcca gtggaggttg aaccggtggt
      421 atcaagggtt aatgccacgc tcacttctga cgtggtgatt aagtctaccg aaatattatg
      481 ccgcgcgagg atgccgaaaa cttccgcgag gaagccgcga gaatgcagca tattcaggct
      541 gtgcaaagtg agcagagtct gattgcgacg aagcgccagc gcgcggaaca gcggcggatt
      601 ttcagtttta ttgcacacca gcgtaccacc tgcgcgtggg tctttgctgg agccgacaaa
      661 gaccgggata tcgctgcgta ctgcgggtag caacgttgcc ggatgcagta cttttgcacc
      721 aaaagttgcc atctctgccg cttcggcaaa cgcgatttca tcaatgcgtt ttgctgcgga
      781 aactacgcgt ggatcggtgg tgtagatgcc cgggacgtcg gtccagatat caacacgaga
      841 tgcgtgtaaa gcctccgcca gcaaggctgc cgtataatcg ctgcctccac ggccaagcgt
      901 cgttgtacga cctttatttt cgctaccgat aaatccctgg gtgatcacta agccttcatt
      961 gaggcgtggg agcagctgca gcgcggccag ttccgccagc gcggctacat ctggctctgc
     1021 acgaccaaat cggtcgttgg tacgcatcac tttacgtaca tcaaaccact gtgcctgaac
     1081 atcgcgttcg cgcaggattt cgacaaacag cagggtcgac atcagctcgc cgtggctgac
     1141 cagctcatct gtcagcgccg gagacgttgc cagcgccgcc gcttctgcca gaacagtaat
     1201 gttctccagc agacgttcaa tctcttcacg gataacgttc gggtaacgca gacgttccag
     1261 aatggcaaac tggatgttgc gaatagcgtc gagtttttcg aatcgctcgc caagttccag
     1321 cccttccgct aaagcgacca gcagattagt gataccagca gaagccgaga ggactactaa
     1381 acgcacgttg gcatcagaaa gcacaatatc agcgctgcgg ttcatggcgt caaaatcagc
     1441 tacgctggta ccgccaaatt tggagacaac aatttcagac ataactacct cgtgtcaggg
     1501 gatccatttt cagccttggc acaagggaag agcggaagac gggtgggcgc agagcgatac
     1561 ttcgctacta ttttcaccca gaagtgctcc accacttgcg aaacgcccga ctgcgaacgc
     1621 ttctggtgac aacccagggg attcagcccc tgtagccgat gatgaacgtg gccagccgtt
     1681 caatcacctc ggcgatgcac cccctcaggt gttatcacag gactggctcc tccaacaccg
     1741 ttacttgggc aacgcgcctc ttctggcctg cgctagcgca ggtagtacat ttataaataa
     1801 agggtgagcg gggcggttgt caacgacggg gtcatgcgga tttttcatcc actcctggcg
     1861 gtcagtagtt cagctaataa atgcttcact gcgctaaggg tttacactca acattacact
     1921 aacggcacta aaaccatcac atttttctgt gactggcgct acaatcttcc aaagtcacaa
     1981 ttctcaaaat cagaagagta ttgctaatga aaaacatcaa tccaacgcag accgctgcct
     2041 ggcaggcact acagaaacac ttcgatgaaa tgaaagacgt tacgatcgcc gatctttttg
     2101 ctaaagacgg cgatcgtttt tctaagttct ccgcaacctt cgacgatcag atgctggtgg
     2161 attactccaa aaaccgcatc actgaagaga cgctggcgaa attacaggat ctggcgaaag
     2221 agtgcgatct ggcgggcgcg attaagtcga tgttctctgg cgagaagatc aaccgcactg
     2281 aaaaccgcgc cgtgctgcac gtagcgctgc gtaatcgtag caataccccg attctggttg
     2341 atggcaaaga tgttatgcca gaagtcaacg cggtgctgga gaagatgaaa accttctcgg
     2401 aagcgattat ctccggtgag tggaagggtt ataccggcaa agcaatcact gacgtagtga
     2461 acatcgggat cggcggttct gacctcggcc cgtacatggt gaccgaagca ctgcgtccgt
     2521 acaaaaacca cctgaacatg cactttgttt ctaacgtcga tgggactcac atctcggaag
     2581 tgctgaaaaa agtaaacccg gaaaccacgc tgttcctggt agcatctaaa accttcacca
     2641 ctcaggaaac catgaccaac gcccatagcg cgcgtgactg gttcctgaaa gcggcaggtg
     2701 atgaaaaaca cgttgcgaaa cattttgcgg cgctctcaac caatgccaaa gccgttggcg
     2761 agtttggtat tgatactgcc aacatgttcg agttctggga ctgggtcggc ggccgttact
     2821 ctttgtggtc agcgattggc ctgtcgattg ttctctccat cggctttgat aacttcgttg
     2881 aactgctttc tggcgcacac gcaatggaca agcatttctc caccacgcct gccgagaaaa
     2941 acctgcctgt cctgctggcg ctgattggca tctggtacaa caatttcttt ggtgcggaaa
     3001 ctgaagcgat tctgccgtat gaccagtata tgcaccgttt cgcggcgtac ttccagcagg
     3061 gcaatatgga gtccaacggt aagtatgttg accgtaacgg taacgttgtg gattaccaga
     3121 ctggcccgat tatctggggt gaaccaggca ctaacggtca gcacgcgttc taccagctga
     3181 tccaccaggg aaccaaaatg gtaccgtgcg atttcatcgc cccggctatc acccataacc
     3241 cgctctctga tcaccaccag aaactgctgt ctaacttctt cgcccagacc gaagcgctgg
     3301 cgtttggtaa atcccgcgaa gtggttgagc aggaatatcg cgatcagggt aaagatccgg
     3361 caacgcttga ctacgtggtg ccgttcaaag tgttcgaagg taaccgcccg accaactcca
     3421 tcctgctgcg cgaaatcact ccgttcagcc tgggtgcgtt gattgcgctg tatgagcaca
     3481 aaatctttac tcagggcgtg atcctgaaca tcttcacctt cgaccagtgg ggcgtggagc
     3541 tgggtaaaca actggcaaac cgtattctgc cagagctgaa agatgataaa gaaatcagca
     3601 gccacgatag ctcgaccaat ggtctgatta accgctataa agcgtggcgc ggttaatcat
     3661 catgcgtgcc tgatgccgat atgtaggccg gataaggcgt ttacgccgca tccggcaacc
     3721 ggagcctgat gccaatatcg acattaatgc atagtatgag gagttaaata accttaaaaa
     3781 caatgccgac aataagtcgg cattgtttta tcagataaat ccccttgtct gtgatttaac
     3841 ggaaatcata ccgtgaggtt aatcctaaaa tagattttta atcgttgttt atttcggaga
     3901 atacgcagat taattacttt tgtttttatt ttaagcttat gatttttatt gttatttaaa
     3961 tataagttga aacttatatt tgatattcat tccaattatc ctaaaactcc atcgctaatt
     4021 ccccgcgccg taattcgcat gctttagttg tgtatactcg atcccgcccg aaatgttttt
     4081 gggtaaatct ccattcattc aatgaaggga aattgttatg aaaaaagttc tgtatggcat
     4141 ttttgccata tctgcgcttg cggcgacttc tgcgtgggct gcacctgtac aggtgggcga
     4201 agcggcagag tcggcagcaa cgtcggtttc ggcggggagt tcctccgcga ccagcgtcag
     4261 caccgtaagc tcggcggtgg gtgtcgcgct cgcggcaacc ggtggcggtg atggttctaa
     4321 taccgggacc atcacaacca cgaccaccag tacccagtaa taaagtatgt atccccaaaa
     4381 taatttgagt cattgcatct gtggcttgaa gtatgaaggg atttaccata accacactcc
     4441 ggtgtggtta ttctgcccct ctggagaaga gtcgtgaagc gacctgcact cattcttatc
     4501 tgcctgctat tacaggcctg ttcagccacg actaaagagc tgggcaattc actgtgggac
     4561 agtctgttcg gcacgccagg cgtacagctg acggacgatg atattcaaaa tatgccctac
     4621 gccagccagt acatgcagct taatggcggg ccgcaattgt ttgtggtgct ggccttcgct
     4681 gaagacggac aacaaaaatg ggtcactcag gatcaagcca ctcttgtcac acaacatggt
     4741 cgtctggtga agactttgct tggcggcgac aacctgattg aagtgaataa ccttgccgcc
     4801 gacccgctga ttaaacccgc acaaattgtt gatggtgcaa catggacgcg cacgatgggc
     4861 tggaccgagt accagcaggt acgctacgcc accgcacgct cagtcttcaa atgggatggc
     4921 accgataccg tcaaggtcgg cagcgatgaa accctggttc gcgtgctgga cgaagaagtc
     4981 tccaccgacc aggcgcgctg gcataaccgc tactggatcg acagcgaagg gcaaattcgc
     5041 cagtcggaac agtatctcgg cgcggattat ttcccggtga aaaccactct catcaaggcg
     5101 gcaaaacaat gattaaacaa actattgtcg cgttgctttt gagtgtggga gcgtcatcgg
     5161 tcttcgcggc aggaaccgtc aaggtgttca gcaatggcag cagtgaggcc aaaacgctaa
     5221 cgggcgcaga gcatttaatc gatctggtag ggcaacctcg actggcaaac agctggtggc
     5281 ctggtgcggt gattagcgaa gagctggcaa cggcggcagc attgcgtcag cagcaggcgt
     5341 tgctgacacg gctggcagaa cagggcgcag attccagcac cgacgatgcc gccgccatta
     5401 acgccttacg ccagcaaatc caggcattga aagtgacagg caggcaaaaa atcaatcttg
     5461 atcccgatat cgtccgcgtt gccgaacgcg gtaacccgcc gttgcagggc aactacacgc
     5521 tgtgggtcgg accaccgccg tccacggtca cgttgttcgg gcttatcagc catcctggca
     5581 atcagccatt cactccaggt cgcgacgtgg cgagctatct ctctgaccag agcctgctca
     5641 gcggtgcgga tcgcagctac gcgtgggtcg tttacccgga cggacgcacg caaaaagcgc
     5701 cggtggctta ctggaacaag cgtcacgtag agccgatgcc cggcagcatt atttatgttg
     5761 gcctcgcaga ttccgtctgg agtgagacgc ctgatgccct taacgccgac attcttcaga
     5821 ctctgacgca gcggataccc caataatgaa aaaaagacat ctgcttagct tactggcgct
     5881 gggcattagc acagcttgct acggcgaaac atatcctgcg cccattggtc cgtcgcagtc
     5941 ggatttcggt ggcgtaggat tattacaaac tcccaccgcg cgtatggcac gggaagggga
     6001 gttgagtctg aactatcgcg ataacgatca gtaccgttat tactcagctt cagtgcaact
     6061 cttcccgtgg ctggaaacaa cgctgcgcta caccgacgtg cgcacccggc agtacagcag
     6121 cgtcgaagcg ttctctggcg atcaaacgta taaagataaa gccttcgatc tcaaactgcg
     6181 tttgtgggaa gagagttact ggctgccgca agtggcggtt ggtgcgcggg atatcggcgg
     6241 tacggggctg tttgatgcgg aatatcttgt tgccagcaaa gcctgggggc cgttcgattt
     6301 tacgctcggc ctgggctggg gatatctggg caccagcggt aatgtaaaaa atccgctctg
     6361 ttcagccagt gataaatatt gctatcgcga taacagctac aaacaggcgg gatctatcga
     6421 cggtagccag atgttccacg gtcctgcctc actgtttggc ggcgtggaat accagacgcc
     6481 ctggcaaccg ctgcgtctga aactggagta tgaaggtaat aattatcagc aggattttgc
     6541 cgggaagctg gagcaaaaaa gtaagtttaa cgtcggggcg atttatcgcg ttaccgattg
     6601 ggccgacgtt aaccttagct atgaacgtgg caacaccttt atgtttggcg tcacgctgcg
     6661 caccaacttt aacgatctgc gcccgtccta catcgataac gcccgcccgc aatatcaacc
     6721 gcagccgcag gatgccattt tgcagcattc ggtagtggcg aatcagttaa cgttgctgaa
     6781 atacaacgcc ggacttgccg atccgcagat ccaggcgaaa ggcgatacgc tgtatgttac
     6841 cggcgagcag gtgaaatatc gtgattcgcg cgaagggatc atccgtgcta atcggatcgt
     6901 gatgaacgat ctgccggatg ggatcaaaac gatccgcatt acggaaaatc gccttaacat
     6961 gccgcaagtg acgacggaaa ccgatgtcgc cagcctgaaa aatcatctcg ccggagagcc
     7021 gttgggccac gaaacgacgc tggcgcacaa acgcgtcgag ccagtggttc cgaagtccac
     7081 cgagcagggc tggtatatcg acaaatcacg ctttgatttc catatcgatc cggtgctgaa
     7141 ccagtcggtc ggtggcccgg aaaactttta catgtatcag ctgggcgtga tgggaacggc
     7201 agatttgtgg ctgacggacc atctactgac caccggcagc ctgtttgcaa atcttgccaa
     7261 caactacgac aagtttaact acaccaatcc accgcaggac tcgcacttac cgcgcgttcg
     7321 tacccatgtg cgcgagtatg tgcagaacga tgtctatgtg aataacctgc aagccaacta
     7381 cttccagcat ctgggcaacg gcttctacgg tcaggtctat ggaggttatc tcgaaaccat
     7441 gtttggcggt gcgggggccg aagtgttgta tcgtccgctg gacagtaact gggcgtttgg
     7501 tctggatgcc aactacgtta aacagcgcga ctggcgtagt gcaaaagata tgatgaaatt
     7561 caccgactac agcgtgaaaa ccggacatct gaccgcctac tggacgccat ctttcgccca
     7621 ggacgtgttg gtgaaagcca gcgtcgggca gtatctggca ggggataaag gcggcacgct
     7681 ggagatcgct aaacgctttg acagcggcgt ggtggtgggt ggctatgcca cgatcaccaa
     7741 cgtttcgaaa gaggagtacg gcgaagggga cttcaccaaa ggcgtgtatg tctcggtacc
     7801 gttggatctc ttctcgtctg gcccgacacg cagccgtgcg gcgattggct ggacgccgct
     7861 gacgcgtgac ggtggtcagc aacttgggcg taagttccag ttgtatgaca tgaccagcga
     7921 ccgtagcgta aatttccgct aagtcatggg aaaggtgcca gttttcgcac tcactggcac
     7981 cctcattctt aatgacctgt tgagccatca ggaacgcagt tcacccccgg tttacgatct
     8041 ggtgtgtaaa ggcgaaaaca ttctgtcttc ctgtccgtcg atatcggttg caggtctggt
     8101 tgcctgagcg actgggccat cattgggctg gagacattgc tttgcggtag gtcggcttta
     8161 tcagcagtac cgagcggacc agcataagca ggaagaacag agactgataa catcaaagca
     8221 gcaaaataag gcttcatttt taccaccttt atcaggttac gtttcatttg ttccagagga
     8281 acattgtcga tttttcgcgc attgctggtg gctgggaatc acctgaatgg gtgatttttg
     8341 aattaccggc tttggtgcgg tttgtcttgc cggatgcgcc gccaggcgcg gcttatccgg
     8401 cctacgggta ggtatatccg gctttggtgg aggcgcgctc caaatccagg ttgaacaaaa
     8461 catacacaaa aaatatagat ctccgtcaca tttttgcgtt atacaggaag ctcgccactg
     8521 tgaaggaggt actgctatga cgtcactctc tcgtccgcgc gtggagttta tctccaccat
     8581 tttgcagacc gtactcaatc ttggcctgct gtgcctcggc ctgattttgg ttgtcttcct
     8641 cggcaaagaa acggtgcatc tggctgatgt gctgttcgcg ccagaacaaa ccagcaaata
     8701 tgagctggta gaaggactgg tggtttactt tctctatttc gaatttatcg cgctgattgt
     8761 gaagtacttt cagtccggtt ttcacttccc gctacgttac tttgtctata ttgggatcac
     8821 cgccattgtg cgcttgatca tcgtcgatca taaatcgcca ctggatgtgt tgatctactc
     8881 ggctgcgatc ctgttactgg tgatcaccct gtggctgtgt aattcgaaac ggctgaagcg
     8941 ggagtaaaaa gacagcacgc cgaaatggcg cggcgtgctg gacaggaaga ttacagcgta
     9001 gcagtttgtt gtgttttctt cgtttccggt tcccagagcg cttccagctc ctcaagggtt
     9061 ttacctttgg tttccgggac aaatttccac ataaacagtg ctgccagaac gcccatacaa
     9121 ccgtaaatcc agtaggagaa accgttgtgg aaatgggcca ccagccagga gtttttgtcc
     9181 atcatcggga aggtccagga gacgaagtag ttcgccagcc actgggccgc caccgcgatt
     9241 gccagcgctt taccacgaat agcattcggg aagatttccg acagcagtac ccagcatacc
     9301 ggaccccagg acatggcaaa ggcggcaaca tagaacagca tcgacagtag cgccacaata
     9361 cccgatgcct gagtgtaaaa cgcggtaccg aggctaaaca taccgattgc cattccgagt
     9421 gcgccgataa tttgcagtgg cttacgacca aatttatcca cagtcataat tgccagtacg
     9481 gtaaaggtga ggttgataac tccgacaata atggtctgca acagcgcgat atccgtgctg
     9541 gcccccagcg ttttgaacac ttccggcgcg tagtacagca ccacattgat gccgacaaat
     9601 tgctggaaga tggagagcat aacgccgatt acaatcacgc ccacgccaaa catcagcaga
     9661 cgaccaccgg ttttgcggcc atgatccagg gagtgtttaa tttcctgtac tgcctgagtt
     9721 gcaagcgtgt tgcccataat tttgcgcagg atactttccg tctgttcttg cttgccgcgt
     9781 gacatcagcc agcgaggact ttctggcacg gtatacagca gcattaagaa cagcagtgca
     9841 gggatacatt ccgaggcaaa catataacgc ctgccgtcag tattcagcca gctggcatca
     9901 ccggaacggg caataaaata gtttacgcag taaactaaaa gttgcccgaa aataatcgca
     9961 aactggttaa atgaaaccag ttttccgcga atatgagctg gagccagttc cgcaatatac
    10021 attggtgaaa gcattgaggc taaaccaacg ccaataccgc caataatacg ataaataaca
    10081 aattccggga cataacctgc cagataaata ggtactgtgt tgtccgggtt tatagaggta
    10141 aaaccaagtt ctggccaggc agaacctaca ccagaaataa aaaacaggac agcagaaatc
    10201 ttaagtgaat cacgacggcc gaagcggtta ctgcaataac caccgagggc accgccgatg
    10261 atgcaaccaa tcagagcgct ggctacgcaa aatcctaaca gggagttggc agcggattca
    10321 cttaagtttt gtggagcaac aaagacggta ttgagtgact caacagtacc ggaaataacg
    10381 gcggtgtcgt agccaaataa taaaccacct aatgtagcga ctaaggtaat cgaaaatata
    10441 taactggaat tatactgggt attcattcag acctgcctta gaccattctg atcttttatc
    10501 agtgcattga tgttggaatt gtcaggcagt taaggcaatt atcatttttt acaatgcgtt
    10561 tacgtaatgt cttttctgtg atcttaattg tgataattat caaaaattga tgaaaacaat
    10621 actattgccg tgactcagag cacgaaagag aattatcgta agtgggaaaa caaataacgt
    10681 aaaaataata agttctatga tgggaatatt aaccggcgaa cgattcagat tgcagacgaa
    10741 agaaaaa
//
LOCUS       DAEEUE010000121        10724 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.121, whole genome shotgun sequence.
ACCESSION   DAEEUE010000121 DAEEUE010000000
VERSION     DAEEUE010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10724
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.121"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            75..1475
                     /locus_tag="KLK84_003470"
     CDS             75..1475
                     /locus_tag="KLK84_003470"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBE2010664.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            1631..3166
                     /gene="gadC"
                     /locus_tag="KLK84_003471"
     CDS             1631..3166
                     /gene="gadC"
                     /locus_tag="KLK84_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HBE2010665.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            3297..4616
                     /locus_tag="KLK84_003472"
     CDS             3297..4616
                     /locus_tag="KLK84_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HBE2010666.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            4914..4955
                     /locus_tag="KLK84_003473"
     CDS             4914..4955
                     /locus_tag="KLK84_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010667.1"
                     /translation="MVQLVDLARCVSF"
     gene            4993..6138
                     /gene="dosC"
                     /locus_tag="KLK84_003474"
     CDS             4993..6138
                     /gene="dosC"
                     /locus_tag="KLK84_003474"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HBE2010668.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            6063..6680
                     /locus_tag="KLK84_003475"
                     /pseudo
     CDS             6063..6680
                     /locus_tag="KLK84_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            6938..7270
                     /gene="ddpX"
                     /locus_tag="KLK84_003476"
                     /pseudo
     CDS             6938..7270
                     /gene="ddpX"
                     /locus_tag="KLK84_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            7284..8180
                     /locus_tag="KLK84_003477"
                     /pseudo
     CDS             7284..8180
                     /locus_tag="KLK84_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
     gene            8177..9163
                     /locus_tag="KLK84_003478"
     CDS             8177..9163
                     /locus_tag="KLK84_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE2010669.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            9156..10082
                     /locus_tag="KLK84_003479"
                     /pseudo
     CDS             9156..10082
                     /locus_tag="KLK84_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(10138..10569)
                     /gene="osmC"
                     /locus_tag="KLK84_003480"
     CDS             complement(10138..10569)
                     /gene="osmC"
                     /locus_tag="KLK84_003480"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HBE2010670.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2607 a         2647 c         2706 g         2764 t
ORIGIN      
        1 gtcctttgca cttgcttact ttatcgataa atcctacttt tttaatgcga tccaatcatt
       61 ttaaggagtt taaaatggat aagaagcaag taacggattt aaggtcggaa ctactcgatt
      121 cacgttttgg tgcgaagtct atttccacta tcgcagaatc aaaacgtttt ccgctgcacg
      181 aaatgcgcga cgatgtcgca tttcagatta tcaatgatga attatatctt gatggcaacg
      241 ctcgtcagaa cctggccact ttctgccaga cctgggacga cgaaaacgtc cacaagttga
      301 tggatttatc cattaacaaa aactggatcg acaaagaaga atatccgcaa tccgcagcca
      361 tcgacctgcg ttgcgtaaac atggttgccg atctgtggca tgcgcctgcg ccgaaaaatg
      421 gtcaggccgt tggcaccaac accattggtt cttccgaggc ctgtatgctc ggcgggatgg
      481 cgatgaaatg gcgttggcgc aagcgtatgg aagctgcagg caaaccaacg gataaaccaa
      541 acctggtgtg cggtccggtg caaatctgct ggcataaatt cgcccgctac tgggatgtgg
      601 agctgcgtga gatccctatg cgccccggtc agttgtttat ggacccgaaa cgcatgattg
      661 aagcctgcga cgaaaatacc atcggcgtgg tgccgacttt cggcgtgacc tacaccggta
      721 actatgagtt cccgcaaccg ctgcacgatg cactggataa attccaggcc gacaccggta
      781 tcgacatcga catgcacatc gacgccgcca gcggtggctt tctggcaccg ttcgtcgccc
      841 cggatatcgt ctgggacttc cgcctgccgc gtgtgaaatc gatcagtgct tcaggccata
      901 aattcggtct ggctccgctg ggctgcggct gggttatctg gcgtgacgaa gaagcgctgc
      961 cgcaggaact ggtgttcaac gttgactacc tcggtggtca gattggtact tttgccatca
     1021 acttctcccg cccggcgggt caggtgattg cacagtacta tgaattcctg cgccttggtc
     1081 gtgaaggcta taccaaagta cagaatgctt cctaccaggt cgctgcctat ctggcggatg
     1141 agatcgccaa actggggccg tatgagttca tctgtaccgg tcgcccggac gaaggcatcc
     1201 cggcggtttg cttcaaactg aaagatggtg aagatccggg atacaccctg tacgacctct
     1261 ctgaacgtct gcgtctgcgc ggctggcagg ttccggcctt cactctcggc ggtgaagcca
     1321 ccgacatcgt ggtgatgcgc attatgtgtc gtcgcggctt cgaaatggac tttgctgaac
     1381 tgttgctgga agactacaaa gcctccctga aatatctcag cgatcacccg aaactgcagg
     1441 gtattgccca gcagaacagc tttaaacata cctgataacg tttaacggta acggtgtccc
     1501 gaaacgaacc cgtttcggga taatttccaa agtctgttca ctggcattag caacggaaaa
     1561 tattgttctg aatacgcttc agaacaaaac aggtgcggtt ccgacaggaa taccgtttta
     1621 gggggataat atggctacat cagtacagac aggtaaagct aagcagctca cattactcgg
     1681 attctttgcc ataacggcat cgatggtaat ggctgtttat gaatacccta ccttcgcaac
     1741 atcgggcttt tcattagtct tcttcctgct attaggcggg attttatggt ttattcccgt
     1801 gggactttgt gctgcggaaa tggccaccgt cgacggctgg gaagaaggtg gtgtcttcgc
     1861 ctgggtatca aatactctgg gtccgagatg gggatttgca gcgatctcat ttggctatct
     1921 gcaaatcgcc attggtttta ttccgatgct ctatttcgtg ttaggggcac tctcctacat
     1981 cctgaaatgg ccagcgctga atgaagaccc cattaccaaa actattgcag cactcatcat
     2041 tctttgggcg ctggcattaa cgcagtttgg tggcacgaaa tacacggcgc gaattgctaa
     2101 agttggcttc ttcgccggta tcctgttacc tgcatttatt ttgatcgcat tagcggctat
     2161 ttacctgcac tccggtgccc ccgttgctat cgaaatggat tcgaagacct tcttccctga
     2221 cttctctaaa gtgggcacac tggttgtatt tgttgccttc attttgagtt atatgggcgt
     2281 agaagcttcc gcaactcacg tcaatgaaat gagcaacccc gggcgcgact atccactggc
     2341 tatgttactg ctgatggtgg cggcaatctg cttaagctct gttggcggtt tgtctattgc
     2401 gatggtcatt ccgggtaatg aaatcaacct ctccgcaggg gtaatgcaaa cctttaccgt
     2461 tctgatgtcc catgtggcac cggaaattga gtggacggtt cgcgtgatct ccgcactgct
     2521 gttgctgggt gttctggcgg aaatcgcctc ctggattgtt ggtccttctc gcgggatgta
     2581 tgtcacagcg cagaaaaacc tgctgccagc ggcattcgct aaaatgaaca aaaatggcgt
     2641 accggtaacg ctggtcattt cgcagctggt gattacttct atcgcgttga tcatcctcac
     2701 caataccggt ggcggtaaca acatgtcctt cctgatcgca ctggcgctga cggtggtgat
     2761 ttatctgtgt gcttatttca tgctgtttat tggctacatt gtgttggttc ttaaacatcc
     2821 tgacttaaaa cgcacattta atatccctgg tggtaaaggg gtgaaactgg tcgtggcaat
     2881 tgtcggtctg ctgacttcaa ttatggcgtt tattgtttcc ttcctgccgc cggataacat
     2941 ccagggtgat tctaccgata tgtatgttga attactggtt gttagtttcc tggtggtact
     3001 tgccctgccc tttattctct atgctgttca tgatcgtaaa ggcaaagcga ataccggcgt
     3061 cactctggag ccaatcaaca gtcagaacgc accaaaaggt cacttcttcc tgcacccgcg
     3121 tgcacgttca ccacactata ttgtgatgaa tgacaagaaa cactaaccat gtctgaatgg
     3181 ttataagaca agggagcgat aattcatcgc tccctttttc gtgcttgctg tcgtattgac
     3241 ttaaccggga taagtacgag aatgagcgca catctgttta ccggaaacca gcacatatgg
     3301 atatctgctc ccgaaacaag aaattaacga ttagaagacc agcgatactg gttgcattgg
     3361 cacttttact gtgtagttgt aaaagcacgc ctccagagtc catggtgaca ccaccagcag
     3421 gttcaaagcc accagccacg acgcaacaat cgtcacaacc gatgcgtggc atctggctgg
     3481 ccacggtttc tcgcctcgac tggccaccgg tttcctcggt taacattagt aatcccacca
     3541 gccgagcccg tgtacaacaa caggcgatga tcgacaaact ggatcatctc caacgtctcg
     3601 gcataaacac ggtctttttc caggtcaagc cggacggtac cgccctgtgg ccatcgaaaa
     3661 ttttgccgtg gtccgatctt atgaccggta agattggtga aaatccgggt tacgatccgc
     3721 tgcaattcat gctcgacgaa gcccacaagc gtgggatgaa agtacacgcc tggtttaacc
     3781 cctatcgcgt atcggttaat acgaagcccg gtactatcag ggaactgaat agcactctgt
     3841 ctcaacaacc ggcgagcgtc tatgtgcaac accgtgactg gatcagaacg tctggcgatc
     3901 gctttgtcct cgacccgggc atccctgagg ttcaggactg gatcacatca atagtcgctg
     3961 aagtggtttc ccgctatccg gtagatggcg tgcagtttga cgactatttc tatactgaat
     4021 caccgggttc acggctaaat gataacgaaa cgtaccgtaa atacggaggc gcatttgcgt
     4081 caaaagcaga ctggcggcgc aacaatactc agcagttaat tgcaaaggta tcacacacca
     4141 ttaaaagcat taagccggga gtcgaatttg gcgttagccc ggcaggcgtg tggcgtaacc
     4201 gatcacacga tccgctcggt tccgataccc gaggcgcggc agcctatgac gaatcctacg
     4261 cagacacccg tcgatgggtg gaacaaggat tgctggatta cattgctccc caaatttact
     4321 ggccgttctc acggagtgcc gcgcgttatg acgtgttggc aaaatggtgg gcggatgtcg
     4381 ttaaaccaac caggacccgc ctgtatatcg gtatcgcctt ctataaagtg ggtgaacctt
     4441 caaagataga gccagactgg atgattaacg gcggcgtacc ggaactgaaa aagcagctcg
     4501 atcttaacga tgcggtacca gaaattagcg gcaccatctt gttccgtgag gactatctga
     4561 ataaaccgca gactcaacaa gcggtcagct atctgcaaag tcgttggggc agttaaacta
     4621 ccatgcttac tttgtaacaa gccggtgctt tacccaccgg cttgttacac cttgtgaaat
     4681 atcccactag taatcatgct tacataagtc aaattaacca cgcaattaac cacacttagt
     4741 taaacagcta taaatcccta ataaaaatgt atgaatatga aaaaccatac gatattcgct
     4801 gtcaccgaaa aataattacg atgggctata ctcttctgaa gaacagtagc gtagattgaa
     4861 actgtcttaa gtcgcttatt tcttttcagt atatcttcat atttcaggag aatatggtgc
     4921 agttagtcga tctcgcacgt tgcgtttcgt tttgattccg tctgatttcc cagccttata
     4981 agggtgtgaa ttatggagat gtattttaaa agaatgaaag atgagtggac cggacttgtc
     5041 gaacaggcag atcctctcat tcgtgctaaa gccgcagaaa ttgcccttgc gcatgctcat
     5101 tatctgagta tcgagtttta tcgaattgtc cgcatcgacc cgcatgccga agaattcttg
     5161 agtaatgaac aagttgagcg gcagttgaaa agtgcgatgg aacgctggat tattaacgtg
     5221 ctttctgccc aggttgacga tgtcgaaagg ctaatacaaa tccagcatac cgtcgcggaa
     5281 gtgcatgccc gcataggaat tccggtagaa attgtcgaga tggggtttcg ggtgctgaaa
     5341 aaaatcctct atccggtaat cttctcttcg gattattccg ccgcagaaaa acttcaggtc
     5401 taccatttct cgattaacag tattgatatc gcgatggaag tgatgacccg cgcgtttacc
     5461 tttagtgaca gtagtgcctc aaaggaagat gaaaactatc gtatcttctc gttactggaa
     5521 aacgccgaag aagaaaaaga acggcaaata gcctcaatac tttcatggga aatagatatt
     5581 atctataaag tcctgctgga ttctgattta ggcagcagtt tgcctttaag ccaggctgat
     5641 tttggcctgt ggtttaacca taaaggtcga cattatttta gcggcattgc cgaagtgggc
     5701 catatctccc gtctgattca ggatttcgac ggtattttca atcaaaccat gcgtaacacc
     5761 aggattttga ataacagaag tttgcgggta aaatttctat tacagataag aaataccgta
     5821 tcgcagatta ttaccttgct gcgtgaattg tttgaagaag tatcgcgcca cgaagtcggt
     5881 atggatgtac tgacgaaatt acttaaccgc cgtttcctgc cgactatctt caaacgcgaa
     5941 attgcccatg ccaaccggac cggtacaccg ctgtcagtgc tgattattga cgttgataaa
     6001 ttcaaagaga tcaacgatac gtggggccat aacactggcg atgaaattct gcgtaaagtc
     6061 tcattcctct cgcagaagag attggtgaaa tcgaaaatat tgggcgctgg gtcatcgcgg
     6121 aagcttgccg tcagttagca gaatggcgta gccagaatat tcatatcccg gcgttatccg
     6181 tgaacttgtc ggcgctgcac tttcgcagta atcagctgcc taatcaggtg tctgatgcaa
     6241 tgcaagcctg gggtattgac ggccaccagc tgacggtgga aatcacggaa agcatgatga
     6301 tggaacacga taccgaaatc tttaagcgca ttcagatcct gcgtgatatg ggcgtgggtt
     6361 tatcggtaga tgattttggc acgggctttt ccggattatc ccgcttagtc agtcttccgg
     6421 taacggaaat caaaattgac aaaagctttg tcgatcgttg tctgacggaa aaacgcatcc
     6481 tggccttact tgaagccatt accagcattg ggcaaagcct caatttaacc gtcgtggcgg
     6541 aaggggtcga aaccaaagag caatttgaga tgctacgtaa gatccactgt cgcgttattc
     6601 agggatattt cttttcccgc ccccttcccg ccgaagaaat tccaggctgg atgagcagcg
     6661 tgttaccgct gaaaatctga caaattcctc tcgcccgcac tcgcgggttt tcctttaagg
     6721 tgacactgtc acttaaatac tgtgatttca gctacgattc cgggagattc cttccttaac
     6781 tttccctctt gcaccaaaac tgcactacgc tgcacaaatg ttgatcaaaa gttaaaaaat
     6841 ataactttaa gccagcattc tgccgcttat cccggcatgg catgagatct gcataagcgg
     6901 aaagcgcagc aatttttgtc ttatacagga accgtttatg tcggatacca ccgaactggt
     6961 tgatttagcc gtgatcttcc ctgatctgga gatcgaattg aaatatgcct gcgttgataa
     7021 catcacaggt aaagctattt atcagcaagc gcgttgtctg ctacataaag atgcgattac
     7081 cgcgctggcg aaaagtatca gtatcgccca gctgtcaggc ttacaactgg tgatttacga
     7141 tgcgtatcgc ccacaacaag cacaggcgat gttgtggcaa gcctgcccag acccgcaata
     7201 tgttgttgat gtgacggtcg gttctaatca cagccgtggc acggcgctcg acctgacgct
     7261 tcgtgatgac gtggaggtgg tgaatgatgc taagcgagga aacgtccgcc gtacgcccac
     7321 aaaaacaaac gcgatttaac ggtgcaaaac tggtatggat gctgaaaggc agtccgctca
     7381 ccgtgaccgg cgcagtcatc attgtattaa tgcgactgat gatgattttt tcaccgtggc
     7441 tggcgacgca tgatcccaac gccattgatt taaccgcccg ccttttgccg ccttctgcgg
     7501 cgcactggtt tggcaccgat gaagtgggac gcgatctgtt tagccgcgta ctggtcggca
     7561 gtcagcaatc aattctcgcc ggattagtgg tggtcgccat tgcgggtatg attggttcgc
     7621 tactcggatg tctatccggt gtgcttggcg gacgcgcaga cgccatcatc atgcgcatca
     7681 tggacattat gctgtcgatt ccttcgttgg tactgacaat ggcactggct gccgctctcg
     7741 ggccgagttt atttaacgcc atgctggcga ttgctattgt gcgaattccc ttttatgtgc
     7801 gcttggcgcg ggggcaagca ttagttgtac gccagtatac ctatgttcag gcggcgaaaa
     7861 cctttggcgc gtctcgttgg catctgatca actgacatat tttacgtaac tccctaccgc
     7921 cgctgatcgt gcaggcatcg ctggatatcg gtagcgcgat tttaatggcc gccacgttgg
     7981 gatttattgg cctaggtgct caacaaccga gtgctgaatg gggcgcgatg gtagcgaatg
     8041 gtcgcaacta tgtactcgat cagtggtggt attgcgcatt tccgggggca gcgattttgc
     8101 ttacctccgt cgggtttaat ctctttggtg atggtattcg cgatctgctt gacccgaaag
     8161 caggaggaaa gcagtcatga cccaacccgt tctggacatt caacagctgc atttgagttt
     8221 ccccggtttt aacggtgatg ttcacgcgct caacaatgtg tccttgcaga ttaaccgcgg
     8281 cgaaattgtc ggtctggtgg gagaatccgg ctcaggtaaa tcagtcaccg caatgctgat
     8341 tatgcgtctg ctaccgacgg gcagttattg cgtacatcgg gggcagattt cactgctggg
     8401 agaagatgtt cttaacgctc gggaaaaaca gcttcgtcag tggcgcggcg cacgagtggc
     8461 gatgatcttt caggaaccga tgaccgccct taatccgaca cgtcgaatag gtcttcagat
     8521 gatggacgta atccgccatc atcaaccaat aagtcgtcgg gaagccagag ctaaagcgat
     8581 tgccctgctg gaagagatgc aaatcccgga tgccgtggaa gttatgtcgc gctatccgtt
     8641 tgagctttca ggtggtatgc gccagcgggt aatgattgcg ctggcattct cctgcgagcc
     8701 gcaattgatt attgccggcg aaccgacgac ggcgctggac gtcacagtac agttgcaggt
     8761 actgcgtttg cttaaacata aagcacgcgc cagtggaact gcggtactgt tcatcagcca
     8821 tgatatggcc gtggtgtcgc aactgtgcga tagcgtttac gtgatgtatg ccggaagcgt
     8881 aattgaaagc ggcgggacgg cagacgttat ccatcatccc cggcatccgt ataccattgg
     8941 tttgctgcaa tgcgcaccag aacatggagt accacgccag ccattacccg ccattccagg
     9001 gacggtacca aacctcaccc atttgcctga cggctgcgct tttcgcgatc gttgctatgc
     9061 ggcaggtgca cagtgtgaaa acgtcccggc gctgacagcg tgtggtgaca acaaccagca
     9121 ctgcgcctgt tggtatccgc agcaggaggt cataagtgtc tgacacgtta ttaacgttac
     9181 gcgacgtcca tatcaatttt ccggcccgta aaaactggct tggtaaatct acggaacatg
     9241 ttcatgccat taatggtatt gatttacaga tccgccgtgg tgaaacctta gggatcgtcg
     9301 gcgagtcagg ctgcggcaaa agcaccctcg cacagctttt aatgggcatg ctgcaaccga
     9361 gccacgggca gtacatccgt tcaggctcac aacgcattat gcagatggtg tttcaggacc
     9421 cgctctcttc gcttaatccg cgcttaccgg tgtggcgcat catcacagaa tcgctctgga
     9481 tagctaagcg tagtagtgaa caacagcggc gagcgttggc agaggagttg gctgtgcagg
     9541 tgggtattcg tccggagtat ctcgaccgcc tgcctcatgc gttctccggc gggcagcggc
     9601 aacgcatcgc cattgccaga gcactctctt cgcagcctga cgtgattgtg cttgatgagc
     9661 caacctcggc gctggatatc tccgtgcagg cgcagatcct caatttgctg gtaacgctac
     9721 aggaaaatca cgggctgact tatgtgctga tttcacacaa tgtctcggtg atacgtcata
     9781 tgagcgatcg ggtggcggtg atgtatctcg ggcagattgt agaactgggc gacgcgcaac
     9841 aggtgttgac tgcttcggca catccataca cccgattatt gctggattcc ctccccgcca
     9901 ttgataaacc gctggaggaa gaatgagcat tacgtaaaac ggatctgccg ggaaaccgca
     9961 cgttgccgca aggctgtttt ttctacgaac gttgccctct ggcaacccac ggatgtgaag
    10021 tccggcaatc attagcaata agggaggacg gacgtgagct ccggtgctgg cgggcgctgt
    10081 agagatgcta acaacgcatc aggcattcac gcgcctgacg cgtcatccgg caatgcttta
    10141 cgatttcaac tggtaatcca gcgtaatttc cgctttcagt acctgagaga ccgggcatcc
    10201 tgcttttgct ttctggatga tgccgtcaaa ggtagaggca tcaatacccg gcaccgcaac
    10261 ttcactcttc agtgcgattt tcgtaatcgc aaaaccggca tccactttat ccagcgacac
    10321 atcggcggtg gtatcaattg atgttggcgt gaatcccgct tcccccagca ttaatgaaag
    10381 cgccattgag aaacatgcgg catgcgctgc gccaatcagt tcttcagggt tggttccttt
    10441 ttcgccttca aaacgcgtgt taaatccata cggctgttgg ttcagcacgc cgctctcggt
    10501 ggatactgtt cccttcccgc gtttgatatc gccttcccag tgtgcctgac ctttcttatg
    10561 gattgtcatt gttgctctcc tgtgggctta aaaaggaaat tatagactac gtgagaaaca
    10621 gcacgaggat aagcaggata ttccgaataa aatacggcga aaatattact tctgcactta
    10681 aattaaattc ccacaataga taaccgggaa tatatatctc aaga
//
LOCUS       DAEEUE010000122        10687 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.122, whole genome shotgun sequence.
ACCESSION   DAEEUE010000122 DAEEUE010000000
VERSION     DAEEUE010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10687
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.122"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..213)
                     /locus_tag="KLK84_003481"
     CDS             complement(<1..213)
                     /locus_tag="KLK84_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HBE2010671.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSS"
     gene            704..835
                     /locus_tag="KLK84_003482"
     CDS             704..835
                     /locus_tag="KLK84_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HBE2010672.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            1095..2090
                     /gene="pgl"
                     /locus_tag="KLK84_003483"
     CDS             1095..2090
                     /gene="pgl"
                     /locus_tag="KLK84_003483"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HBE2010673.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2131..3084)
                     /locus_tag="KLK84_003484"
                     /pseudo
     CDS             complement(2131..3084)
                     /locus_tag="KLK84_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            3268..4320
                     /locus_tag="KLK84_003485"
     CDS             3268..4320
                     /locus_tag="KLK84_003485"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HBE2010674.1"
                     /translation="MKKIPCVMMRGGTSRAAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            4396..5829
                     /locus_tag="KLK84_003486"
     CDS             4396..5829
                     /locus_tag="KLK84_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HBE2010675.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AMALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            6012..8192
                     /locus_tag="KLK84_003487"
     CDS             6012..8192
                     /locus_tag="KLK84_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HBE2010676.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMASGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(8333..9616)
                     /locus_tag="KLK84_003488"
     CDS             complement(8333..9616)
                     /locus_tag="KLK84_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HBE2010677.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(9751..10632)
                     /locus_tag="KLK84_003489"
     CDS             complement(9751..10632)
                     /locus_tag="KLK84_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE2010678.1"
                     /translation="MTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMSKIKAI
                     RRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPV
                     AATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCEMKWSD
                     IVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLS
                     SGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMA
                     SQYRDDRGREWDKIEIK"
BASE COUNT         2623 a         2462 c         2663 g         2939 t
ORIGIN      
        1 cgaggaaagc gccaaggctc gcaggtccag tttatgcccc ttagaggtaa gacatgcacg
       61 aagtcttctt accgcctcag tacgctgttc ttccggtaac ccctgtttct cccaatcatc
      121 ccattcggac aaataatggt tgggggatgt aattgtgctg atatgtggaa ataaacagga
      181 tgaaatgttt ctgagcatat taatttctcg catatggata tggaattaga ttgtgaataa
      241 attgtattta atgtatctct cctaatgagg agggtgtttc agaatattcg ccataaggga
      301 atgagtgtta tatcatgctt tctaccctgg taagtatatt tgcttttttc gtatatttgg
      361 ttcatctttt tttgatgttt tacatattca caatgcagag gatttatacc agaggacatt
      421 agtcttgagt aagatactac aacatctcca actattttag ccagtctgac tacttgactt
      481 ggtaatattt gttgctaata atcatgtata aaataagctt atatacatag taacttcagc
      541 aaaggattta gtaatgatca aaatcatatc tcaatgtact gtttttttct ttaaaaaaac
      601 agtacattct attttataat gactgtgtat tcccatggaa ggagatcacc caattctccc
      661 ccccgacttt tgacgcagtg tcttgttaac agggggattg accatgctta ttggctatgt
      721 acgcgtatca acaaatgacc agaataccga tctacaacgt aatgcgttta tttgtgccgg
      781 aagtgagttg attgttgcgc gaatgggaag ttggccagaa gttgttatgg catagtgttt
      841 acggtcgttg atacaggtaa acaaaagcgg ggagagtcat actccctgaa tagtcgcgaa
      901 aatatctgtg gcattgtcaa attcgtgtcg gtgactaata ttttagtaat atgccattcg
      961 aaatggagtg ctaacttatt gatttaattg atgtgcgata ttgtatttta tcgtgttttg
     1021 ttgtctgttt tttatccagt aagtgttgtt atatgtatga aagataaggg atttatcatt
     1081 tttgaggtgt gttcatgaag caaacagttt atatcgccag ccctgagagc cagcaaattc
     1141 acgtctggaa cctgaatcat gaaggcgcac tgacgctgac acaggttgtc gatgtgccgg
     1201 ggcaggtgca gccgatggtg gtcagcccgg acaaacgtta tctctatgtt ggtgttcgcc
     1261 ctgagtttcg cgtcctggcg tatcgtattg ccccggacga tggcgcactg acctttgccg
     1321 cagagtctgt gctgccgggt agtccgacgc atatttccac cgatcaccag gggcagtttg
     1381 tctttgtagg ttcttacaat gcgggtaacg tgagcgtaac gcgtctggaa gatggcctgc
     1441 cagtgggcgt cgtcgatgta gtcgaggggc tggacggttg ccattccgcc aatatctcac
     1501 cggacaaccg tacgctgtgg gttccggcat taaagcagga tcgcatttgc ctgtttacgg
     1561 tcagcgatga tggtcatctg gtggcgcagg accctgcgga agtgactacc gttgaagggg
     1621 ccggcccgcg tcatatggta ttccatccaa acgaacaata tgcgtattgc gtcaatgagt
     1681 taaacagctc agtggatgtc tgggaactga aagatccgca cggcaatatc gaatgtgtcc
     1741 agacgctgga tatgatgccg gaaaacttct ccgacacccg ttgggcggct gatattcata
     1801 tcaccccgga tggtcgccat ttatacgcct gcgaccgtac cgccagtctg attaccgttt
     1861 tcagcgtttc ggaagatggc agcgtgttga gtaaagaagg cttccagcca acggaaaccc
     1921 agccgcgcgg cttcaatgtt gattacagcg gcaagtatct gattgccgct gggcaaaaat
     1981 ctcaccacat ctcggtatac gaaattgttg gcgagcaggg gctactgcat gaaaaaggcc
     2041 gctatgcggt cgggcaggga ccaatgtggg tggtggttaa cgcacactaa ccgctgattt
     2101 aaccggcgca gtctctcctg cgccggtgta ttaacttatc tcctgtaacg catgtctctg
     2161 gcgttcgacg atattggtcc acaaattgtc tttatcgtca gtccacaaat taatcagcaa
     2221 ggaaaaaaag cgttcagctg ccggagaaag cacggcatct ttgcggcgaa taatccccaa
     2281 tgtccgacgt ataacgggtt ccaccagtgg gataccaata atcgaagaat agggcgcatg
     2341 gggcattgcc agaccaggaa gcgccgaaat ccccagtcct gcctccacta gtcctaatga
     2401 cgtcgaaaga tgacgcactt cgtaaaacca gtccagcttc cagggcttgt cggctagctg
     2461 ttgttctatc aacaatcggt tgccgctgga ggaacgtacg ccaatcattt tgtaacccac
     2521 taactcctgc cattctacaa gctacttttt ggccaatgga tggtcacgcc gacaggccag
     2581 cacgaatggc tcgttgacca gtggggtaaa atcaatggat gaatttgtga cgttgttcat
     2641 gtttatgcca aagtctgatt cgttacacag caccgattcc atgcaattat tcgtgccttg
     2701 ttccagaatc cgcactttaa tattgggata cagctcatta aatttaccga ttgccagcgg
     2761 taaaaaataa aatactgcgg tcggaataca cgctaatgtc accatatcac gatgataagc
     2821 attcatatca cgaatattaa aaagcgtttc atcgaatttc tttattaatt cccgcgcctc
     2881 cgggagcaac ctttttcctg cttttgttaa cgtaaccttg cgagttgtac gctcaaatag
     2941 ttgaatatgt aaatcttcct ccatcttttt tattcttcgc gttaatgcag gttgcgtaat
     3001 attgagtaat ttagcggcat tgttaaatga actggactct gccagtatga caaatgcctt
     3061 catacttgat aattcatgct tcattattac tccggaaaat ggaagcgacg attttggggg
     3121 gctggtcgtt aaaaatttta actgcattta gccaacttaa attaatgaaa aaatgttatt
     3181 aatcgttgag ctaaagtcat tagagatgct ttgtccttaa tgtaaccata tcgcaataag
     3241 ttatgttttt aaattgaggg cattattatg aaaaaaatac cctgcgtgat gatgcgaggt
     3301 ggaacctcga gggccgcgtt cctgttagcg gaacatttac ccgaagatca aacgcagcgc
     3361 gataaaatat tgatggcaat tatgggttcc ggtaacgatc tggaaattga cggtattggc
     3421 ggcggtaatc cactgaccag taaagtcgcc attattagcc gttccagcga tccgcgtgct
     3481 gatgtcgatt atctgtttgc tcaagttatc gtccatgagc aacgtgtcga taccacgcct
     3541 aactgcggca atatgctgtc tggcgtaggg gcattcgcca ttgaaaatgg tttgattgca
     3601 gcgacttcgc cagttacccg tgtacgtatc cgcaacgtca atacgggtac gttcatcgaa
     3661 gctgatgtgc aaacgccaaa tggtgttgtc gagtacgagg gtagcgccag aattgacggc
     3721 gtaccgggta ctgccgcacc ggttgcgctc actttcctga atgccgctgg aacgaaaacc
     3781 ggaaaagttt tcccgactga taatcagatt gattattttg acgatgtccc ggtgacctgt
     3841 atcgatatgg cgatgccagt cgtcattatt ccggctgaat atctgggaaa aacaggttat
     3901 gaattaccgg cggaactgga tgccgacaaa gcattattag cccgcattga atctatccgg
     3961 ctacaagcgg gtaaagcaat gggcttaggt gatgtcagta atatggttat ccctaaacct
     4021 gtgcttattt ctccagcgca gaaaggcggg gcaattaatg tgcgttattt tatgccgcat
     4081 tcttgccatc gcgcgctggc gataaccggt gctattgcta tttccagtag ttgtgcattg
     4141 gaaggcaccg tcacccgaca aatcgtccct tctgtaggat atggcaatat caatattgaa
     4201 caccccagtg gtgcgctcga cgttcattta agtaatgaag gtcaggatgc cacgacgtta
     4261 cgcgcatctg ttattcggac aaccagaaaa atattttccg gtgaagttta tcttccctga
     4321 aaaaattcgt tgtcaggata aggacaatca ataaaggact tctgtatgag tcatacagaa
     4381 agaacaggat tttaaatgaa taagaaatcg ttatggaagc taattctgat attagcgatc
     4441 ccatgtatta ttggttttat gccagctccg gcaggattaa gcgaactggc gtgggtgctt
     4501 tttggtattt acctggcggc cattgtgggt ctggttatca agcctttccc ggaacctgtc
     4561 gtactgttaa ttgccgttgc tgcctcaatg gtggtggtcg gtaacttatc cgacggtgcg
     4621 tttaagacca ccgccgtatt aagcggttac tcttcaggta ctacctggct ggtgttctcg
     4681 gcgtttacct taagcgccgc atttgtgacc accggtttag gtaaacttat tgcctatctg
     4741 ctgattggta aaatcggtaa caccatgctg ggtctgggtt acgttacggt attcctcgat
     4801 ctggtactgg ctccggcaac accgtctaac accgcgcgtg cgggcggcat tgtgttaccg
     4861 atcatcaaca gcgtggcgat ggctttgggg tccgaaccgg aaaaaagtcc gcgtcgtgtc
     4921 ggacattacc tgatgatgtc catttacatg gtcaccaaaa ccaccagcta tatgttcttt
     4981 accgcaatgg cggggaacat tctggcgctg aaaatgatca acgacattct gcacctgcaa
     5041 attagctggg gtggatgggc gctggccgcc ggattgccgg gcatcattat gctgctggtc
     5101 accccgctgg taatttacac catgtatcca ccagaaatta aaaaggtgga taacaaaacc
     5161 atcgctaaag cgggccttgc cgaactggga ccgatgaaaa tccgcgaaaa aatgctgctc
     5221 ggtgtctttg tgctggcgct actgggctgg attttcagta agtctctggg ggttgatgaa
     5281 tccactgtgg caatcgttgt tatggcgacc atgctgctgc tgggtatcgt tacctgggaa
     5341 gacgtggtta aaaataaagg cggctggaat accttaatct ggtacggcgg tattatcggc
     5401 ttaagctcct tattatcgaa agttaaattt ttcgaatggt tagctgaagt ctttaaaaat
     5461 aacctggcat ttgatggtca cggtaacgtt gctttcttcg ttattatttt cctcagcatc
     5521 atcgtgcgtt atttcttcgc ttccggtagt gcctatatcg ttgctatgtt acctgtattt
     5581 gccatgctgg cgaacgtctc cggcgcaccg ttaatgttaa ccgcgctggc actgttgttc
     5641 tccaactcct atggcggcat ggttactcac tatggcggcg cggcaggtcc ggtcatcttt
     5701 ggcgtgggtt acaacgatat taaatcctgg tggttggtcg gtgcggtact gacgatatta
     5761 accttcttgg tgcatatcac cctcggcgtg tggtggtgga atatgctgat cggctggaac
     5821 atgctgtaaa tatactcgtc atacttcaag ttgcatgtgc tgcgtctgcg ttcgctcacc
     5881 ccagtcactt acttatgtaa gctcctgggg attcactcac ttgtcgcctt cctgcaactc
     5941 gaattattta gagtatatcc atttattatc tttctgcgca cttcacggtg cgcagatatc
     6001 tggagcattt gatgatcaag ttatctgaaa aaggcgtgtt tctcgccagt aataacgaaa
     6061 taattgccga agaacatttc accggcgaaa ttaaaaaaga agaagcaaaa aaaggcacta
     6121 ttgcctggtc tattctctct tctcataata cgtccggaaa tatggataaa cttaaaatta
     6181 agtttgattc attagcctct cacgatatta cctttgttgg tattgtacag accgctaaag
     6241 cgtccggaat ggaacgtttc ccgctgccgt atgtgctgac caactgccat aactcactct
     6301 gcgccgtcgg cggcaccatt aacggtgatg accatgtttt tggtttatcg gcagctcagc
     6361 gttatggcgg tatttttgtg cctccgcata ttgcggtcat ccatcaatat atgcgtgaga
     6421 tgatggctag cgggggcaaa atgatcctcg ggtcagacag ccacacccgt tacggtgcat
     6481 tagggacaat ggcagtcggt gagggtggcg gtgagttggt aaaacagctg cttaatgaca
     6541 cctgggatat cgactatccg ggcgtggttg cggtgcatct gaccggaaaa cctgcgccgt
     6601 atgtggggcc acaggatgtg gcgctggcta tcattggcgc ggtgttcaaa aacggttacg
     6661 tcaaaaacaa agtcatggag ttcgttggac cgggcgttag cgcgctctct accgatttcc
     6721 gtaacagcgt tgacgtgatg accacggaaa ccacctgttt aagctccgtc tggcaaactg
     6781 atgaagaagt gcataactgg ctggcgctgc acggtcgcgg ccaggattac tgccagctta
     6841 accctcaacc gatggcgtac tacgatggct gcatcagcgt tgatttaagc gccatcaaac
     6901 caatgattgc gctgccgttc cacccgagca acgtgtatga aatcgacaca ctgaaccaga
     6961 acctgaccga cattctgcgt gagattgaaa ttgagtccga acgcgtggcg cacggtaaag
     7021 ccaaactctc gctgctggat aaagtggaaa atggtcgcct gaaagtgcag caggggatta
     7081 tcgcgggctg ttctggcggt aactacgaaa acgtcatcgc ggcggcgaat gcactgcgcg
     7141 gtcaatcctg tggcaatgac accttctcgc tggcggttta cccgtcatca cagccggtgt
     7201 ttatggatct cgccaaaaaa ggtgtggtag cagatttgat tggcgcaggc gcaatcatca
     7261 gaaccgcatt ctgtggccca tgctttggcg cgggcgatac gccaatcaac aacggtttga
     7321 gtattcgcca caccacgcgt aacttcccga accgcgaagg ctctaagcca gccaatgggc
     7381 agatgtcagc ggtggcgttg atggacgctc gttctatcgc tgcgactgcg gcaaacggtg
     7441 gctatttaac ctctgccagc gaacttgatt gctgggacaa cgtgccggag tacgccttcg
     7501 atgtaacgcc gtataaaaac cgtgtttatc agggctttgt gaaaggggca actcagcaac
     7561 cgctgattta cggaccgaac attaaagact ggccggaatt gggtgcgctg actgacaata
     7621 tcgtcctgaa agtgtgctcg aagatcctcg acgaagtcac caccaccgac gaactgattc
     7681 cttccggtga aacctcttct tatcgttcaa atccgattgg tctggcgggg aatgtcagcg
     7741 agctgacaga ggtgtttgcg cgcattaagc agattgctgg tcaggagcat attgatccgc
     7801 tgcaaactga aattggcagc atggtctatg cggtgaaacc aggcgatggt tctgcgcgtg
     7861 aacaggcggc gagctgccag cgtgtgattg gcggtctggc gaatattgcc gaggagtacg
     7921 cgactaaacg ctaccgttct aacgtcatca actgggggat gttaccgctg cagatggcgg
     7981 aagtgccaac ctttgaagtg ggggattaca tttacatccc tggcattaaa gcggcgctgg
     8041 ataatccggg tacgacgttt aaaggttatg tgatccatga agatgcgccg gtaacggaaa
     8101 ttacgcttta catggaaagc ctgacggctg aagagcgcga gattatcaag gcgggtagtt
     8161 tgattaactt caataaaaac cgtcagatgt aaaaagcgcc atgtgaatgt aggtcgcatt
     8221 cggcacttat tgtcggatgc gatgctggcg catcttatcc gacctacaaa tcgcatcgaa
     8281 ccgtaggccg gataaggcgt ttacgccgca tccggcaaat agttaatagc tcttacttct
     8341 tcgcctctgc aaccactttg ctgcccacgc cgcggttatt gtattcccac atgcggttgt
     8401 agttagtgtc attcagattg cgctgtattt cgtcgttatc atctacgctg ccggtattac
     8461 ccgcaaacgg acgattagag atcaccgcat cggcccacgg tttagccgtg ttaaaacctt
     8521 cgttgatggc gctatcacgg atcaccacct gaccgttggt attagcatca acatccagcg
     8581 agcggcccag ttgcgccacg ccatcaccgg aagcattgaa acggctgttt acggcgagga
     8641 aaccgtaata gatgttagac agcgtagccg gtgcaaacac atatgcttct tgctgggtac
     8701 gtgagttcac cacgcggaat tcggtgttat cgaacaccac tgcgccgcga ccagaaacga
     8761 tatccacatc cccttcaata tagctgttgg tcaccagcgt acgcggctga cggttggttt
     8821 ccagacggtt ctgcacaccg ctgttggtga caaagaaggt gttctgacga ccgagaatgt
     8881 taacgttgtt aatctgtact tggtcaccgt cagtacgcag tgctaccgcc ggatggttac
     8941 ctgcatctac gctatcgccc agcgtgtttt cgatggtcag attttgcagt tgcagtccat
     9001 tgttttgtga ccagaagacc gcagaacaca gaacaccgat actgtcgctg cgtttgctct
     9061 ggcagctatc gtacatatac cacgctggtt tacctggcat atatttgccg cgcgggttga
     9121 cgtcgtgacg ccagtcggca gggctcatgc ccccatcaag ggaaagccca atcttcacat
     9181 caatcggttt ttcacctgta ccgtacagag tgattccacc tggagcagca gggacataca
     9241 ccgttccctg atactcccca ggcatcacgg caatatactg gcgcttgttg gtgcgcttga
     9301 taattgccgc atctaccgcc gcctgaatcg tggtatgcgt tacaccttga gtacccgccg
     9361 ggccgacaac aaagtcaggt tgcgcaggca gggttatcgg ggacggattc cacgctgcag
     9421 cacctggtgt cagggatgca aaatagtgtt gcgcatcgaa attcagcgct tcttttgccg
     9481 acagaatcgg gcgagaagag gtaccaggcg cagtttgatc agaaggacgt tgatcgggtg
     9541 gtgttgagct acaggcggtc agcgtcacgc caaaagccaa tgccagcgcc agacgggaaa
     9601 ctgaaaatgt gttcacaggt tgctccgggc tatgaaatag aaaaatgaat ccgttgaagc
     9661 ctgctttttt atactaagtt ggcattataa aaaagcattg cttatcaatt tgttgcaacg
     9721 aacaggtcat tatcagtcaa aataaaatca ttatttgatt tcaattttgt cccactccct
     9781 gcctctgtca tcacgatact gtgatgccat ggtgtccgac ttgtgcccga gaagatgttg
     9841 agcaaactta tcgcttatct gcttctcata gagtcttgca gacaaactgc gcaactcgtg
     9901 aaaggtaggt ggatcccctt cgaaggaaag acctgatgct tttcgtgcgc gcataaaata
     9961 ccttgatact gtgccggatg aaagcggttc acgacgagta gatgcaatta tggtttctcc
    10021 gccaagaatc tctttgcatt tatcaagtgt ttccttcatt gatatcccga gagcatcaac
    10081 atgcaatgtt gtagggatgg caatttttac gcctgttttg ctttgctcga cataaagata
    10141 tccatctacg atatcagacc acttcatttc gcataaatca ccaactcgtt gcccggtaac
    10201 aacagccagt tccattgcaa gtctgagcca acatggtgat gattctgctg cttgataaat
    10261 tttcaggtat tcgtcagccg taagtcttga tctccttacc tctgattttg ctgcgcgagt
    10321 ggcagcgacc gggtttgttg ttatatggcc ttcagctatt gcctctcgga atgcatcgct
    10381 cagtgttgat ctgattaact tggctgatgc cgccttgccc tcgtctatgt atccattgag
    10441 cattgccgca atttcttttg tggtgatgtc ttcaagtgga gcatcaggca gccccctcct
    10501 tattgcttta attttgctca tgtaatttat gagtgtcttc tgcttgattc ctctgctggc
    10561 gaggattttt tcgtagcgat caagccatga atgtaacgta acagaattat cactgttgat
    10621 tctcgctgtc agaggcttgt gtttgtgtcc tgaaaataac tcaatgttgg cctgtatagc
    10681 ttcagtg
//
LOCUS       DAEEUE010000123        10534 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.123, whole genome shotgun sequence.
ACCESSION   DAEEUE010000123 DAEEUE010000000
VERSION     DAEEUE010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10534
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.123"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(99..371)
                     /locus_tag="KLK84_003490"
                     /pseudo
     CDS             complement(99..371)
                     /locus_tag="KLK84_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
     gene            complement(465..1027)
                     /locus_tag="KLK84_003491"
                     /pseudo
     CDS             complement(465..1027)
                     /locus_tag="KLK84_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1231..1437)
                     /locus_tag="KLK84_003492"
     CDS             complement(1231..1437)
                     /locus_tag="KLK84_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HBE2010679.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1544..2107)
                     /locus_tag="KLK84_003493"
     CDS             complement(1544..2107)
                     /locus_tag="KLK84_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HBE2010680.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3482..5317)
                     /locus_tag="KLK84_003494"
     CDS             complement(3482..5317)
                     /locus_tag="KLK84_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010681.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5418..5705
                     /locus_tag="KLK84_003495"
     CDS             5418..5705
                     /locus_tag="KLK84_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBE2010682.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5677..7206
                     /locus_tag="KLK84_003496"
     CDS             5677..7206
                     /locus_tag="KLK84_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HBE2010683.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7376..8593)
                     /locus_tag="KLK84_003497"
     CDS             complement(7376..8593)
                     /locus_tag="KLK84_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE2010684.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8960..9946)
                     /locus_tag="KLK84_003498"
     CDS             complement(8960..9946)
                     /locus_tag="KLK84_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010685.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(9943..10471)
                     /locus_tag="KLK84_003499"
                     /pseudo
     CDS             complement(9943..10471)
                     /locus_tag="KLK84_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT         3282 a         2412 c         1987 g         2853 t
ORIGIN      
        1 cagaagcaga aaaagcttat tatgcttcca tcggaaacga tgatctggca gcctgagttc
       61 acagataaaa cactctccag gaaacccggg gcggttcact tacaccagac atcgttatta
      121 agtagcagca ccataagata aggagtggca tcgttagcca cagcttccct actccagagg
      181 taatataaag gggtgggctc gactgattta tctttacgtc gcttacactg caaatgttca
      241 gaaattagtc tatgcagctc accagtaaaa tcagcaatca gagaaggaat tgccttattc
      301 ataccagggc aaggtattaa tttaaactgt aataatttaa ttttaggatg tgtagcagca
      361 gcccgataca gagttgcaag gacacacttt tgccagaggg cgttacagga aagcttaatg
      421 tttgattctg tatacataat aaatcacctt atagttacaa taggtcaaaa accgctgtag
      481 ccaaagttac gctggcctga tgctttagtg tcgagcttcg tcaaataatc cagacgtacc
      541 aataagcgct ggtactgctc tgggaaatta ggatcgttaa tatccaggat gtaacgtcca
      601 ttagcaggga aatgaactaa tccctggtga tcttccagtt ccaaccctaa ggcactgtac
      661 caggctccgg taattaaccc tctcagggtg tcagagcgct catagtttcc cagatggtaa
      721 taggcgtctt tgttaaaaaa aggcagacgt gataatgaca tttaaaggac tgcgtatatt
      781 ctttcgccca gataatacaa accgacgttg cgtaggtgcg tttgccttgt tgttttttac
      841 ggtgttgatc tgcattaatt ttcgcattca gcgagttacg gaaacgagat atctctccgg
      901 ggatcaggtt agggaaacag gtaatgatat ccccgttgtc aatcacagga gggtaatgga
      961 gatccccccg tattgctgtt attctggaag tgtttttaat attcgtattg atagttctca
     1021 gagatatatc acactctaga tattttaatt aagtatcagt tcgatatcgg catattgtac
     1081 actacctcag gtagatgatg agatcagata cagggatcaa ctgacggtga gcgcagtccc
     1141 cacattttat ccagggctta ctgcatattc ccaacagcca ttcgttagcc aggtagacga
     1201 ataccctgat ttgccgctgg ttttgctttc ctatcgaagg gcccatacat catcacgccc
     1261 acgaaacaac cgacgaaata acgcaacttt ctcatccgtg gataatacga aaacgcactg
     1321 cacagggctc tgcggtttac gtcgccattc aatcccatga gcttcaagta atgagaccag
     1381 ccgggtattt tctgcccgca gtactgctag ttcatcgctg tcagatttat aaaccatatg
     1441 cgttatcact tgtacctgtc tcccggcggc accacgactg aaattcgtgt gaaggcaaat
     1501 ccacgcgata acttacgata gttcggacat aagcccccat aattcagtta tgtcgtggtc
     1561 gaccccggtt acttttttcg ggaagcctgc tacggcccag aataataccg tgttctttct
     1621 gatcctgttt agccagatag ctaagggcgt aacgtaagtc gtctaccgca gatttatcac
     1681 tatagtgaat cacgtgatcg atacgcaccg ggtatttgtc tttagcccgg cacagatgaa
     1741 aataaccctc cccttccgtg atccgcctcc agatatctcc cagttgcctg gatatctgat
     1801 aagagttctt gtgacgttgt ccatccaggt agccgataaa atgaatatga agccctttgt
     1861 ctggtgtata ttccattacc cagtaatatc ccgccaacat cgtctgggtt tcgctaagta
     1921 aacggtatat ttccatacac atactgtgct tacaggaatg cccgaaactg ggcgtgtctt
     1981 tcctgtaagc aaaatcaatt ctgaaaggta acagtttaga aaaacgttga aacataccat
     2041 ccatgtgttc attcacgtct ttcagaatca tgaaatccat tttgtaattg ggattggcat
     2101 tatacatttt atagtcctta ctttataaaa ctcacaggaa gagagaatta cgtaaatact
     2161 gagagtagag aaacgccgta ccacggcatt aataaacgaa gtaaaccagc agcacagaca
     2221 acctgataaa actgttttgt attaccgcat tactgtgatg aggtaatact tcagaataca
     2281 gtgagactct cttaaactga gaaccagtac gaattacaat ggaaaaacac acctcataga
     2341 attgagtttc aggaagcacc actaaattct ttacaggaag taatctgaca ttcacaaaac
     2401 tcagtatcgg ctagctacag gcaacatcgc tattaatatt gtcgaaccat taagtaaagc
     2461 cgagagagac cacgggtatt taatcattag cataaaagaa taatggtata cagaacatac
     2521 ccacacacca taagaagatt gttccataca gaaaagatca tatttaccta tacctataaa
     2581 ctgtaacgga gtcataacac gctctaatag tgttccgata cgtaaccagt gttaagtacg
     2641 tacaggacaa cattacacac ataattcacc atacagcttc ccccatatgc ttcactgcgt
     2701 agtgtatgga gatatatgca ttacacacat acagaatact tagcataaag aggagtgaca
     2761 ttaccacgta aagagataag ttatacaaca aacatacaaa caatacggca cagacacaca
     2821 ataccataac tccaactaat aatactaatt aaccttaatt atttatggta tatgaaataa
     2881 atgtgattct cccggaacta actaacctaa ccagacagat gatcactgta taaaaacttc
     2941 agttaatact catttgtttt gtcgactcag tatataatga gtgaccagta atcaggataa
     3001 tcacacttac ataacctgta aaaaagaaca agaagaataa gccactaaca taatgcaaaa
     3061 tggcatctga atcgatcgca gtttggcatt cgaatcgatc atgatttggc ccaatccgtg
     3121 atcgaataga gcggaatacg caacataatt caccagacaa gagcttacac cgaattacag
     3181 attatgtatt aatgtcaaaa ctggcatatt ttacagatac aatgaacatg caacccatca
     3241 atctaatagg ggcggtatgt acatctgccc atgaatggga tggcgctaag aatacaaaac
     3301 aattagacat agatcacaaa aacacaccat tctaattgaa aaacaaaaca aacaaataaa
     3361 tcatactatt atagaatttt ctcttgccag acatagataa tacccctgct aattttgcta
     3421 atggttagca aaattagcag ggtatataaa gattattgac ccaaaacaca ctcagatgaa
     3481 ttcacgacag ctcaggaggg ttattcctga tgtcatcttc acaaataatg gttaacgcat
     3541 catatatcat tctgcgtctg ttcctgtttt cggccttatc gccatacaac atcatcacat
     3601 ttttaatttc atcgaccggt gatttcacat agtgaactaa tacaccaagc ttctcaacac
     3661 attgcagtaa agtatatttc ttactgcctg tgaaaagccc tatcatagtt tctttacgtt
     3721 cgcgactgga taatactttg ttttccataa tgacttctgc cagcgtctta ccatcataaa
     3781 catactcttt atctatcaga atatttatct tatccagcac cttctctacg tcatcaagca
     3841 aacctgtaat atatgggggc gcaatatatg gtttcgtact gatcttttca agcatacaat
     3901 tatacatctt aataacacgc atcagggtaa aattgttcgc atcatactgt atttcaccct
     3961 ccttatatac aggatcaata agtggtattt tttcaataaa tgcatcctga taggctttta
     4021 catggtcaag ccaataaaac aacgagccat actttatatt ttttctattt actttaacct
     4081 ccagtgctgt ccgaagcaac gataatgccc ccctgacata tcctgaaggt aattcaccaa
     4141 acaatccttt tctttccatg tcatcataat gctgcagcac atcattaatt ttatttttcc
     4201 ttatcagaca aatgtacacc gcaagtgttt cgtagcagct ggtcacatga acagcctcat
     4261 tctgcaactg cccaaaatag cttataatct ttttcagata tgcgtcatca caaccattct
     4321 tatcattaat attaaataca agatctttca gacgggaaat atattcctga gatttatcat
     4381 caagtgaaac actataacga gacaatgttc cttctgcact gttttgaaac ttataaaaca
     4441 tttccagtaa tttttttgca taaggcgttg tatcaaagct ggtaaagtca taaagatatt
     4501 gcgcagcgta accatacaaa ccatgaatcc agagtccttc tctcaccaac actctgagat
     4561 aatatgcaag gctttgaagt atcttcgtct ttcgttgtat atcaagtaag acacttaatg
     4621 aataaaatgc attatagaat ttatcaatat tcctgagcac tttctccagt tcatccttat
     4681 ccagcttctt ctcccggtaa tcctttttat attcattttt gatagccatg atatattgtt
     4741 gcatcaggct cttgcttttc ccgttatcct tccgtatagt tcctgtatat ttccccagag
     4801 catacagctt tccattttca tgaaaatctt ccttgttgaa tcccagttca gccagatatt
     4861 cccgaagata acgtttaaca gcagcttcat cgctttctgt atcagcgtga tcaagacaag
     4921 gacaatacga tttctgtaaa aaattataaa tatgataata aaaagcagag tcattctctg
     4981 gtctgtatgt attatacata tctacaacaa gaaaaataac cccaaaagtg cgcagacaat
     5041 agctgcgaag gaattcaggt tttagtgtgg aatgcactgg ataagaattc atcatgtaat
     5101 aaaaatgttc atatcgggca tatatgatcc gccgttgctc ctgactgaac accccttcag
     5161 catcaataat acgactgatc tcattataac gttgatccca gaatttttga tgcatttcaa
     5221 aattggtact cttccgcttg tgtgccggat tctcgccctc cttaaacaac cccaaaacag
     5281 tacaaatatc atacaacata tttgtaaatg tgcgcatatg ctgtgctctc cggttggttt
     5341 gcattttatc gcagaggaca ttctgagctt aacgtatcaa actgacacaa cgacatgcaa
     5401 tgcatatgga gaacaatatg tcaaatcaaa ccggacagct ttacacgcct tcacgcatcc
     5461 tgcgtatggc tgaacttacc cgactgctgg gtatatcaag atctactatt tacgtgaaaa
     5521 taaatccggc ctcgaaatac cacgacccta cctttcccaa accaataagg cttggtagtg
     5581 catccgtagg ctggcgggca tctgccatcg atgagtggct gatgttacat acggcaccag
     5641 cctggtctga acctgaaaac aaactaaagg aacttaatga tgacgacaaa actatcactc
     5701 cctgaacttg acacaactaa actgcctgac aggtgccagg cattacttga tgaaatgcat
     5761 gaagaaacag gaatacgccg tgaaatactt ctgtctgtta tgctgacggt catggctgcc
     5821 tcggtccagg atacgcatga agttgaactt tccggagggc aacgtaccag ccttcagata
     5881 tttatgtgtc tttcatcagc atcaggcagt ggaaaaacct ctgcctgcgc aaaattaatc
     5941 gctcctgttc atgaaacaga agaagaacta catcaggcat atatcgacga taaaaaaaac
     6001 tatgaccgaa tgatggaaat gtggacaaca gataaaaaaa tcctggagcg gagatataaa
     6061 aaggaaattg aaagatcccc ggaaaatgct gctgctgcac gagcagcact tgaaaagtgt
     6121 atagcaaata aacccgaacc tcccgtacaa caggttctca tcgtgaatga cgcgacacca
     6181 gaagggatag ctttgaaact cagccagtca ccctctcttc tgttgctgtc tgatgaagga
     6241 ggtactattc tggataagcg tttcgaacgc aaatcggcac tgtacaacac attatggagt
     6301 gggcaaccag tcaacgttga acgggcatcc agacccgggt tccggctgaa tgatgtccgt
     6361 ctcactatac ttatactgac tcaaccggta atatttaata agttttttac tctcactggc
     6421 gatcagatcc ggggcaatgg ctttctggcc cggttgctat tttgcgaacc cggagcaatt
     6481 aaaataatga cgacagaacc gcatactgcc actcctgttg tgacgcaaca atgtgcaagc
     6541 tgttttcgta agaaaagttt tggcagtcag atcagagact ctctgagagc atccagagaa
     6601 agacgtgcaa aaagtaaaga gcgcatctgc atgacattat caaatgcagc atcccgcgca
     6661 ctggagcgct ttcacaaaga aaatatggat actgtcagac agaatcctca tatgggtaca
     6721 tttgaagaca ttattgtcag aaaaagagag caggttgtca ggatagcagc acttctggaa
     6781 ctggagaaag atccagactc gacagtaata acgctggaaa gtactaatag cgccatttat
     6841 ctcattgaat tttatttcaa gcatcttata tataaacttg aatcccttcg ggaaatatcc
     6901 ccggctgaga aacttgataa atggcttcag aaaagaatta tcacaacagc aggttacata
     6961 ttccagaaaa gttatatttt acaatatgcc ccatatgctc tcaggaaaaa atgtgttctg
     7021 gatgaagcac ttgacatact ggcggaacaa aggaaaataa ggattgatga taacctggtt
     7081 gtttatatag gaaacacgat tacaccatcc gaactggcaa aaaaactaaa tattcctgca
     7141 ttcgatgccg gagttttcat ttgtgatcat caaaacatac tgaagtatca ccgaaacaga
     7201 ctatagatac tatcggacaa aaaataaatt atcacaagtg tattattcgt tataataatg
     7261 aataatcgtt atctgagtgc aatgccaggt tttcattacg aactggcatt gcatttattt
     7321 ttcaaaaaag aaagcagtaa ccatttggct actctgagta tgccagtcca tgaaactaag
     7381 cgaattcctt agtgttttta gcaagttgag acatcacata atccgcccat gcctgcatca
     7441 tcggtacacg ctgctccagt agatcagtac gatgatatgc cgcctcaacc ttatttttca
     7501 gcgtatgagc gagcgccctt tccgccagat cccgcgaata cccctgttca ctacaccagt
     7561 ccctgaatgt tgagcgaaaa ccatgtgccg tggcaactcg ccccggaatg tcactgacgg
     7621 ctttcttttt acgcagaaaa cttgtcaaca ccatatcgga aaggatctgc tgctttctgg
     7681 gtgaagggaa caccagttca tcatgcaggc cacgtatatt ttccagaatg taaatagcct
     7741 gccgggataa aggaacacga tgctgtagcc tagctttcat tctttctgca ggtatagtcc
     7801 atacccgctt atgaaaatca atttcagccc agcgcattcc cctggcttcg cccgagcgag
     7861 ttgctgtaag tatcaccatt aataacagtg cgcgggtaac attataaggt tcatcactat
     7921 acacactggt cgccacaaaa agcggtaact gcctccaggg cattgcgggt tggtgttcat
     7981 cacgtcctct tgtctgctga ggaagcaaat ggtcaaccac atcaacagga tttgccacac
     8041 aaaaaccgtg cgcccatccc cactgcataa caacatgaat gcgctgttta acccggcttg
     8101 ccgtttctga caaggttaac cagactggac gcagtgtttc tgccacgtcc gcagccgtaa
     8161 tcgaatccag cgtttttgct cccagttgag gaaacgcgta attctcaagc gtcgataacc
     8221 actgccttac atgctttgga ttctcccatc caggagacag ttctgcatgt acacgcctgg
     8281 ctgcatcggc aaatgttggg ataacgactt tctcagattc agccttttta atctccagag
     8341 gatcatcacc tgcagcaagt tgctctcgca ttatccgggc agtacgtgca gcttcagcaa
     8401 tactgacctc tgggtaagtt cccaatccag cattacgtct tttttgtgtc accggactta
     8461 cataacgaaa aacccatttc ccccgcccct ttactgaaga aggatgaagg gtcagtccgg
     8521 taattccccc atggggcaat ggtttgtcat caggtttgat atgtcttgct ttcgtatccg
     8581 tcaatactgc catacgctaa ttcccgtcac tctggtatgc catccagtat gccattagtc
     8641 cctcgcttca ctaagaatac attagataac atcggacaac aaaaagtgta atacattgtt
     8701 tataaagaaa ttatataaca cccctggatt aagtcagatt tatttcaggc ggtccccctc
     8761 accgccatat ttaaagaaga gcgcgtacga aagtacgggc ttttttttcg tatattgtac
     8821 acaccgggtg cccccgccca cgtctgttag ggcgagggga aattgtgcac atcaactgtc
     8881 tggttactca gtcaacaatc aaaatgcgta agatagtaca gaacatatat gcgttatctg
     8941 gtcacttcct gtaacttcct cacttattaa aatgccccca acggaaatac atgtcacatg
     9001 ccatcataaa atcgttatgc cagaggaatc gacaatggaa aaaatagtaa attaagcacg
     9061 gtacatagca gagtaaaaaa taggcttcaa accaaatccg ccattccaca agcgttgcca
     9121 ataccgacac cagcgtaatc cacatcattt caataacccg caatatgaat aaccgaagcc
     9181 aaagcggctt ggcaaaaaag gtcgacaacg tattttcact ggagaaaaac agatcgattt
     9241 cctgcaaaaa aaacctgccc agaaaagcgg gccgcttgga gaagcgcaga aaaaacagat
     9301 taatcgccaa cactacgcca caaagaatga gcaatgcaat tatcgattta aacgttaacc
     9361 actgcccctc tgtcacaaaa gaatcactgt tacgcaacgg gctgatagta agcaggttaa
     9421 caaccgttga gatgcacacc gaaagcagca catagcgagt cagactttta tattttactt
     9481 ttttcgctac tgaaatagcg acctgcttga cataactggt gcggatcatc cgaaacagca
     9541 gaaagatgac caacggccca agtaacgaaa tcaggaacag cgcatataac gagggtagaa
     9601 gctggtatag cccccaactc attgcactca acatgattaa cgacagaaat cctgccacca
     9661 gaggttgtgt aagaaataat tctattttgt ggttctgctt tttcttaaac cacgaaaaat
     9721 cgatattctt cgtgacgcat ttataagccc agtacgcctt gagatcaaaa ataaaattat
     9781 acagcaccag ggccagaatc ccactaacta caccaatatc ctctgatgga aaagtaaacc
     9841 ctgcattttt ccaataaaag cctatcagaa taagtccaat agcataaatg tatatcagat
     9901 ttcgtttatc ctgaaagcga ataaaagtta aataatcagg aatcatttga atacctttgc
     9961 atcactaaat gctttacgac aatgcataac tcgttgctga tccacgccag caaaatcatt
    10021 taacatattc cccaaatagg gcttattact tatttcaagg aaagctaccg gggttggcat
    10081 attcagaata tgtatcgaga gattcagtcc atttcaccgt atgagttaag tgtaatgaga
    10141 gattttcttt gataattgac ggagcgacct cgctttttgc tgttacgttc atcagaacct
    10201 gatgctctgg cgaagcgata tccagtccgg caagataatc gcgcattgcc tgaacgccgt
    10261 cttccatcaa acgcgtgtgc caggcaccgc tcacaccgag tttaaccggt tcgtatccag
    10321 cggccatcag cagcgtggta aattcattca acgcagcctg cgttccccca ataacctgct
    10381 ggcgcggcgt gttatcacag ctaatatcca gcgcaatgcc tgattccgta atcatcgtct
    10441 gcagttgttc gcggttgatg cctttaaccg cctgatctta cccagcaata gtggacacgc
    10501 ggctaagtga gtaaactctc agtcagaggt gact
//
LOCUS       DAEEUE010000124        10090 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.124, whole genome shotgun sequence.
ACCESSION   DAEEUE010000124 DAEEUE010000000
VERSION     DAEEUE010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10090
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.124"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..177)
                     /locus_tag="KLK84_003500"
     CDS             complement(<1..177)
                     /locus_tag="KLK84_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBE2010686.1"
                     /translation="MNKSLTVLPSGEYPTMSSLEMVDYINADRKSKAEAEGLAFPCKK
                     YRKLEHRSFMKKVPR"
     gene            complement(380..847)
                     /locus_tag="KLK84_003501"
     CDS             complement(380..847)
                     /locus_tag="KLK84_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HBE2010687.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAGEL
                     KLANATITDMQQRQRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYIRTQCLK"
     gene            complement(844..1377)
                     /locus_tag="KLK84_003502"
     CDS             complement(844..1377)
                     /locus_tag="KLK84_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HBE2010688.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(1443..2072)
                     /locus_tag="KLK84_003503"
     CDS             complement(1443..2072)
                     /locus_tag="KLK84_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010689.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNGAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(2076..2291)
                     /locus_tag="KLK84_003504"
     CDS             complement(2076..2291)
                     /locus_tag="KLK84_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBE2010690.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
     gene            complement(2584..2658)
                     /locus_tag="KLK84_003505"
     tRNA            complement(2584..2658)
                     /locus_tag="KLK84_003505"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2623..2625),aa:Gly,seq:tcc)
     gene            complement(2662..2737)
                     /locus_tag="KLK84_003506"
     tRNA            complement(2662..2737)
                     /locus_tag="KLK84_003506"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2702..2704),aa:Thr,seq:tgt)
     gene            complement(2740..2816)
                     /locus_tag="KLK84_003507"
     tRNA            complement(2740..2816)
                     /locus_tag="KLK84_003507"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2780..2782),aa:Arg,seq:tct)
     gene            complement(2818..2894)
                     /locus_tag="KLK84_003508"
     tRNA            complement(2818..2894)
                     /locus_tag="KLK84_003508"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2858..2860),aa:Ile,seq:cat)
     gene            complement(3094..3474)
                     /locus_tag="KLK84_003509"
     CDS             complement(3094..3474)
                     /locus_tag="KLK84_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBE2010691.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
     gene            complement(3467..3667)
                     /locus_tag="KLK84_003510"
     CDS             complement(3467..3667)
                     /locus_tag="KLK84_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HBE2010692.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            complement(3762..4019)
                     /locus_tag="KLK84_003511"
     CDS             complement(3762..4019)
                     /locus_tag="KLK84_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010693.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            complement(4016..5407)
                     /locus_tag="KLK84_003512"
     CDS             complement(4016..5407)
                     /locus_tag="KLK84_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBE2010694.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            complement(5404..6282)
                     /locus_tag="KLK84_003513"
     CDS             complement(5404..6282)
                     /locus_tag="KLK84_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBE2010695.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            complement(6293..7201)
                     /locus_tag="KLK84_003514"
     CDS             complement(6293..7201)
                     /locus_tag="KLK84_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBE2010696.1"
                     /translation="MRPSDLLLDFGHPVAYYPGLVKYMGSPHAVIFFGQIFYWQDKAH
                     AAEGVHKTREEIQHETGLTFEQQAVARKHLVSRGILVETNKRLEHKMFYRIDCERLNE
                     IINENNQFSRNGETRFRETVKPNFAEEGKPSPRTRETPRRGEGKTNFDLTENTTEITS
                     ENTTESKNTIGASADASAPARSSRQEYSPEFEQAWQEYPKRAGGNSKSAAFKAWKARL
                     REGIKPEAMLDGVKRYAAWVRATGNTGTQFVKQAATFFGPDRHFEDFWQQPAAPRGGR
                     QRQVDVLAGLGAMSDKFGKSSDKLTF"
     gene            complement(7188..7421)
                     /locus_tag="KLK84_003515"
     CDS             complement(7188..7421)
                     /locus_tag="KLK84_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010697.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGYVLRVCLVSTESQKVV
                     CQVQGRTHTLSYPLVAFQPGKMFKRLGGGYASV"
     gene            complement(7418..8434)
                     /locus_tag="KLK84_003516"
                     /pseudo
     CDS             complement(7418..8434)
                     /locus_tag="KLK84_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114998.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            8366..8629
                     /locus_tag="KLK84_003517"
     CDS             8366..8629
                     /locus_tag="KLK84_003517"
                     /inference="COORDINATES: protein motif:HMM:NF017757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBE2010698.1"
                     /translation="MRARYQHLVERMKVYGSNLGEPHTSAFGDGLFELRIKGNDGIAR
                     VFYCTLTGKRIIMLHSFVKKAQKTPPAERKKAETRMKEVKHDW"
     gene            8619..8927
                     /locus_tag="KLK84_003518"
     CDS             8619..8927
                     /locus_tag="KLK84_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE2010699.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(8969..9145)
                     /locus_tag="KLK84_003519"
     CDS             complement(8969..9145)
                     /locus_tag="KLK84_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HBE2010700.1"
                     /translation="MRRHRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTT
                     HLWDIRWLRHRARK"
     gene            complement(9142..9552)
                     /locus_tag="KLK84_003520"
     CDS             complement(9142..9552)
                     /locus_tag="KLK84_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HBE2010701.1"
                     /translation="MKQTIFLRTKQQQQAAINAILSTPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            complement(9549..9827)
                     /locus_tag="KLK84_003521"
     CDS             complement(9549..9827)
                     /locus_tag="KLK84_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010702.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQASRAAIELDI
                     DWPESNDDFWKDGEEGAYAMGHEDGKDKTVIAVMKAIRAAGIKEKNFR"
     gene            complement(9902..>10090)
                     /locus_tag="KLK84_003522"
     CDS             complement(9902..>10090)
                     /locus_tag="KLK84_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010703.1"
                     /translation="ASVLMLEKQRNGEGWEGGVPLFLDEQSHQFLQLESGSPYSYIAN
                     MPKSEYDEAWRQENVTEY"
BASE COUNT         2440 a         2707 c         2314 g         2629 t
ORIGIN      
        1 cctgggtact ttcttcatga aactgcggtg ttcgagtttg cgatatttct tacaggggaa
       61 cgcaagcccc tccgcctccg ctttggattt ccgatcagca ttaatgtaat ccaccatctc
      121 aagactgctc atggtcgggt attcgccaga aggaagaacg gttaatgatt tgttcatgtc
      181 ggtatttcct ttagaaagtt gagcctgttc gcacagaaaa gccgtccccg agaggtcctc
      241 acctatacgg cagttctcag gctcagcttt ctgaaagact cgggattgtt acgcgctgcg
      301 atgcgcggtt tactgcggac atataaaagc cccgcaagtg cgaggctcat taaatggact
      361 ttgtgatttg caaaaaaatt tatttcaggc actgagtcct gatgtactcc tgaagcattc
      421 tcaatgctgt ctggtcgctg atgattccgt cccggatacc gagaacgttt cgtccagcaa
      481 ctggagagag ttcgacggtg gcatcattgc ccatgccgga ggcgctggag gtttcggctg
      541 aggatggcac agggcatttt cctttgacga gcacccgacc accattatca agcttgcgcc
      601 gaagagcatc attttcagct ttcgcatcag ctaactcctt cgtgtattta gcatcgagtg
      661 catcagcatc acgctggcgc tgctgcatgt cagtaatggt tgcgttcgcc agcttcagtt
      721 ctccggcgtt tttgtcgcgc tgctctttgt aggtaatggc gttatcacgg taatgattaa
      781 cagcccatga caggcagacg atgatgcaga taaccagagc ggagataatc gcggtgactc
      841 tgctcattga tctatccccc aacaggctaa tgcgctttcc tggtcacgac gaataacctg
      901 tccatagcag ttatttgaac gtatgcggca atcgcgccca acatctttta tccaccagcg
      961 aatcgcctcg catgcgccct tacgatcacc ggcattcagc cgcttataaa acgtcgacgg
     1021 gaaacactta ccggggccaa tgttataggg gcaaaatgac gctatacccg ctttctgggg
     1081 ttcggtcaga ggtactttaa tattgcgctc cacccatgcc agcgccttat cacgttcaat
     1141 agcgttaacc tggtcacatt tttccttcga cagcttcatt cccggtatga caggcttacc
     1201 atccaccatt gtggcaccac gacagatggt ccatatgcca gaaccatcac ggtatgccgt
     1261 tgtgtgatta ccctcttttt cgttcagaaa ctggtcaagt atctggggag cagacgcgcc
     1321 tgcggcaatc agcgccagaa cagcagccga caggccgtat ttgatttttg cgctcatgga
     1381 tatttatcag gatttatcgg caacaaataa cgagccaact tatatacgcc ttttaagata
     1441 agtcagcccc ggctggatcc agtcatcagg ctctttctta aagggggagt attgaaatca
     1501 cgaagaagag cctcccgcac cgcagcatcc atgtctgcac cactggcaag tgcttcaatc
     1561 tctgccgcca cctgcaaata ccccatgcaa cggccaatgc gtttcataac tctggtgttt
     1621 tcgtctttca ggcatctgat agcgagatta ataattttca ggcagtgatg atgcgatgcc
     1681 cggacaggtt ttccgttctc ataaacgacg aactcaccct cttcacagga aaccctgaaa
     1741 ggccgatggg gttcctggtc tttatccggt gcagacgcaa taaaatcact tttatgctct
     1801 tcctctccgg caccgttaac cgtaatgacg tactcaggcc gcaggtaagc cgcctctttt
     1861 tctgacaatg cggccggtaa aaacttttcc tgcaaaaact tcataaaagt ctcgtgagcg
     1921 actttgtggc agtaatcgta aatcgccgca taatgctcat cgcggcgttt gttttcatct
     1981 tcagaactca ccaacgccga caatttttta ttaagctcag tgatttcatt ttccaggtga
     2041 ttgaatcgct gattcatttc ttcatgcttc attatttact ctccccgtgc agccttacgc
     2101 ttgtcttctc ttattttgaa atacagattc gtcagataag tcagaagccc cagaagcaga
     2161 cttcccagca caccaatcgc agcccactgt gatggcctga cctgatccaa ccactgcaaa
     2221 aaccagtagc cggcactgcc ggcggaggtg ccgtaggcaa tgcccgttga aattttgtcc
     2281 atggatttca tagcctcacc tccgcaaata acggatggcg tagttttaca ctgagaaatg
     2341 aaagggattt gaaaagaaaa aaacgcaaaa gcgggcgaaa cgatatatac agtaaggaaa
     2401 gcactctatc caacaaacca cccacagtta atcggaataa aagcagagtg cttatgaatg
     2461 atcgcacgcc cgaaggttag tatttctgca cagcaatttt gcaaaaaaag gcgatcattc
     2521 ataacttaaa cgtctttcag tcactccggg atttcccatc atcgcagact gaaagactct
     2581 gactggagcg ggcagcggga atcgaacccg catcatcagc ttggaaggct gaggtaatag
     2641 ccattatacg atgcccgcat atggtgccga ctaccggaat cgaactggtg acctactgat
     2701 tacaagtcag ttgctctgcc tgctgagcta agtcggcgct ggcccaccac cgaggactcg
     2761 aacctcgcac cgtcaactta gaaggttgat gctctatccg gatgagctag tggtggttgg
     2821 tggcccttgc tggacttgaa ccagcgacct ggcgattatg agtcgctcgc tctgaccaac
     2881 tgagctaaag ggccggaggc agaataataa ccatatgtca tcacatctgc aaactcatct
     2941 gaccaccagc gtgtttaacg tcctgtaccg tttttcaggc ataaaaaacc cgcccggagg
     3001 cgggtttaag ctgtgtggcg aagcaaccac tcttaacaga atatcccgtt ttttacgtac
     3061 gtaaaatatt ttctagaaag tcgcccctta ccatcaggga tgttcaatat atttgtccat
     3121 ttctaaccgg acacccaaca tcatcaacat tccttccacc accccctcgg ctttttgtag
     3181 cttctttcct atataaccat ctgagcatcc atgcttccgc gccagcgtca tgaatgtcat
     3241 cccaaacacg tagtagtcaa ccagcaagtc atgtagatcg ttgttgttcc ggttaaggcg
     3301 ggccatacac ccacatataa ccatcgcgtc atcgtcacag cattgcgggc gggattttac
     3361 ttttgaaggg attaatccct taaaaccggc agcaatggac gaccaggtca catcctcatg
     3421 attattagcc acccacgcgc cccagcgctc aagaacctgc tgaatatcac gcatcactgt
     3481 ctttacccct gtcccatcca cgatgaacaa tcagaacacc atcaacaatg gcgtgccctt
     3541 tgccctcttt atcttcagca tattttctta ccgtggcgcg attacagttc agcattcgtg
     3601 aaacttcggt catattacct cgtgtctgga taagcagttc cggtatcgtt tgaattttga
     3661 cgctcatcaa atactctccg gttcggtgat ttttatcccg ggctttccac cagggacata
     3721 atgatcgcgc acaagccagg gcaaaacaaa ctcatggcgc atcagcgcag cgcctcctgc
     3781 accagttttt caaactttcc gactctggtt tccagctctg ccacacagtc caccagctca
     3841 tctactgctt tctgtgcgcg atgcttcgcc tgcatcagtt cccgaagcgc gggtaccata
     3901 tctttacgga tagcatcttt tgttatgccc gttttttcga gttgttcagc atgacgcagc
     3961 atttcctgcg cgtgtttacg caattgttca ggggtaaaag tcattgtctg gttgttcaaa
     4021 agaaacgctc catcttactg ctgtcagttc gtttattact gtatctgcgc ggattgccgg
     4081 gcttcatggg agtggaaagc acccgtgcac tttcctggtc cacaggcaga aaatgtccgt
     4141 tataaaaacg ccggtaaatc gttcccagag aaccgttacg ttgtttcgtg atattgattt
     4201 ctgcgatgcc cctggcctgc gtatccgggt tgtacacttc atccctgtaa agcatcagaa
     4261 tgatgtctgc atccgcctct atttctccgg aatttttcag gtctgagttc atgggacgtt
     4321 tattgggtct ggactccaca ccgcgggaga gctggctcag cgcaatcaac ggaaaaccac
     4381 cggattttgc caggccttta agcccctttg agatttcacc cacggcaagg tcatgacgcc
     4441 ccgtggttcg ggtttttatc agcccgagat aatcaaccac caccagcgcc gtttccggat
     4501 atttaatcag gtggtgtttc gttgttgcgc atatctcatc aatggccagg ttcgcctggt
     4561 ccaccatcca gatattgcgc ccggtcatcc gccccacccc ttgtgagaaa cgcgtccagt
     4621 cttcatcttc aaagtgagcc acagatttca ggcgtgatac tggcatccct ccagccgcag
     4681 acaccatacg ttcaccaatc tggatgttcg ccatctccat ggtgaacaga agcacaccat
     4741 gcccctgctc agtcaccttg tcgatgatgt ccagcgcaag ttcggttttc cccatcgaag
     4801 gacgggcggc aatgaatacc aggtctccgg gctccatacc gcctgttttt gcgtccagtt
     4861 catcaatacc ggtcatcaac gtcctggatt tctccagccc ctgattccgg cattcaacac
     4921 gctcaaccac ttccggaagc acatcatcaa tatgtaccgg ctgaatgacg ccctttcctg
     4981 tcgacagtgt gaccatcatg ttctgcgcat ccttcagggc atcttcagct gcttcacagg
     5041 tatgcgcatc acgtaatttc tgcagcgcct cattcagtgt tttttctgca tcgcgcagtg
     5101 cggcattgcg ccgcaacgct gcaacatagt gctccagtga agacttcacc caggttttac
     5161 gcccggtatc agtaatcacc ggggcaagtt ccggcatctc attacacaac agcacggggt
     5221 caatcactcc tgaaacacgg gcctgtctgc agatgcctgt atagatatcc tgatacgctc
     5281 gtacagaaaa aacgtccgct ggcagtgtgg ccagaatatc catcacttca ggatctgctc
     5341 cgcgcagaaa gaacgcgccg atgacagcgc cttcaaggtc atcgttacgc catactggag
     5401 ttgtcatgca gccacacctc tgatacgaga acggtaactg ggccagttaa acgacaacca
     5461 gttgcgtccc ccgtctgtga tcctgtcggc aattcgggga ctgatgaacg cccacaactc
     5521 ttccggtgag aggttactga taagaatggt gggcaagata ctttcgtacc tggcgttgat
     5581 aatttcctgc aaaatagcca tttcagccgc gctgccaaac tgaacgccaa cttcgtcgat
     5641 gatcagcaaa tccattgacg cataacgctc aataacttca tccgctgttt tttcgctgtc
     5701 attccgccag caatttttca cagcacgggt aaggcgcatc acgtcggtga tctccacact
     5761 ggccagatag ttacggatga tgtgttttgc catagccaca gccagatgat tttttccggt
     5821 accacaactg ccggtcataa caagactggt accgttctcc agcatatctg gccagttctc
     5881 cgcatagcgg cgacaggccg caagatttct ggctgcgcca ggattaacct ccagataatt
     5941 atcaaactcg cagtcccgaa aacgcagggc aattccggcg ttatcagtca gctcttccgc
     6001 cttgatggac gacagctcca tggtcaaatc gttggcctca gcgattaagc agtcagggca
     6061 gcatgaaatt tcttctctgt cctcgccatt acgatcgctc cacaccagta tatgcgtgtg
     6121 atattcgcca tgtttttcgc aatatccgcg accttcacgc atcaggcagg aacgataagg
     6181 ccatggcttt tcgcccttct gagcaaatgc aatctctgcc cgtaactcat ccattcgcgc
     6241 ctgtagtctt ttttgttgtt cacgcaggtt aaacgtcatc atcgctgtca cctcagaatg
     6301 tcagtttgtc actggattta ccgaatttgt cagacatggc tcccaggcca gccaggacat
     6361 cgacctgtcg ctgtcgccca cctctgggag cggctggctg ttgccagaag tcttcgaagt
     6421 gacgatcggg tccaaagaac gtcgcagcct gcttcacgaa ctgtgtgccg gtatttcctg
     6481 tggcacgtac ccaggcagca tagcgtttca cgccatcaag catggcttca ggttttattc
     6541 cctccctgag ccgggctttc caggctttga aggctgcgga ttttgagtta ccaccagcac
     6601 gttttggata ttcctgccag gcctgttcaa attccggtga atattcctgt cgggaagaac
     6661 gcgctggtgc agacgcgtca gcggatgcgc caatagtgtt tttactctct gtagtattct
     6721 ctgaagtaat ctctgttgta ttctctgtaa gatcgaaatt ggttttccct tctccgcggc
     6781 gaggggtttc ccgtgtccgc ggtgaaggct ttccctcctc cgcgaaattg ggttttacag
     6841 tttcccgaaa acgggtttcc ccatttcggg aaaactgatt gttttcattg ataatttcat
     6901 taaggcgctc acaatctata cggtagaaca ttttgtgctc aagacgcttg ttggtttcaa
     6961 ccaaaatgcc tctggacaca agatgcttac gcgctacagc ctgttgttca aatgtaagtc
     7021 cggtttcgtg ttgtatctct tcacgcgttt tatgtacgcc ttccgctgca tgtgctttat
     7081 cctgccagta aaaaatctga ccaaagaaaa taacagcgtg cggacttccc atgtatttaa
     7141 cgagcccagg gtaataagca accggatgtc caaaatcgag cagaagatca gacggacgca
     7201 tagccacctc ccaggcgttt aaacattttt ccgggctgaa acgccaccag cggataactc
     7261 agggtatgag tacgtccctg aacctggcag acaaccttct ggctttcggt actgaccagg
     7321 caaacccgca gaacgtagcc gttgctggtg gtgaaccact gccccacacg ggggcaatgg
     7381 ttgtatcggt gatacaggaa attaacgatg tggcggatca tgggcgcacc tccttgtcag
     7441 aaccattcag cctggaatca acaagtgcag caccaaaaac agcatcacct acacggtcgt
     7501 acagtttgcc agccagcgga gattcaacgg ccttaagcat tgggtaaagc tggcttgtcc
     7561 agatttgatg gatttcacgc aaatgcaggt atacgcctct ggcgttttgt gcgacagatg
     7621 acatgtcagc cgcaccagct cctgataagc tcctctccat ctggttaaag gcattgatgt
     7681 atgcctcttt gaaccgggcg gcacgtttac cagtgaagcc catagcaagg aaggcgaagc
     7741 cgccgcgggt aatttggtag cagggaagtt tgcgagtacc gccgttgggc tggcgtacca
     7801 aaattgatgt ctccgcaaaa ttgcgggcac aaaactctgg agaacaatcc aaaatgcgga
     7861 tctttttcag aacatcgtca tgacgtttag agaagaagtt ggcaacagcc agggatgaag
     7921 taacagccta accatcaacg atggcaattt caggttgaga gagggttggg agagtagtca
     7981 tggtgacagc ccctatgttg aattcaaaga actcaccaca tgggacgcca atcacagagg
     8041 tggtgagacg tacagggttg gcgtaaccgg tcaacataga acccggcgca tcttgcgatg
     8101 tccctgcacg ccccaccata atttgggcgt agcaatgctc atgacacgaa aaaaccgcat
     8161 gagcgcggtt atgctctata ttgaatttca ggacgccaat cccggccccc gctttataag
     8221 gtgcagagac agtgtaacgt cccgaaattg cagaatcaat atattgtgca gatataacga
     8281 ttttgttatg ttttgctgta tgaactacac tatcgaatac tacagtgaag aggtccgact
     8341 ggaggtcgat cagcttccat tgagtatgcg tgccagatac cagcatcttg ttgaacgcat
     8401 gaaggtatac ggcagcaatc tcggagaacc tcacaccagc gcctttggtg acgggctttt
     8461 cgaactcaga attaaaggca acgatgggat cgcgcgtgtt ttttactgca ctctgacagg
     8521 gaaacgcatc atcatgctgc atagttttgt aaagaaagcg cagaaaacac cgccagccga
     8581 acgcaagaaa gctgaaacca gaatgaagga ggttaagcat gactggtaaa cgcactctcc
     8641 ccaccatgac acacgacgaa atggcagcca aatggatgga agacccggct tttaaagcag
     8701 aatacgacgc tatcgctgac gaattcgcac tgcttgatga aatgctggca gcacgcaaag
     8761 aagctggctt aactcaggct gaagttgccg agcgaatggg aacaaaagcg accgcgatca
     8821 ccagaatgga aagtaatctc gcatcaggta tcagcggccc atcatttgcc acactaaaaa
     8881 aattcgcccg cgctactgga aaaaaactcc agatccgctt cgtttaacca caccgcgccg
     8941 tcattctggc ggcgcaccgg ataaatagtc atttcctcgc acgatgtctt agccaccgga
     9001 tatcccacag gtgagtcgtg tagttgaagg tttttacgtc agattctttt gggattggct
     9061 tgcgtttatt tctggagcgt ttcgttggaa ggtatttgca gttttcgcag atgatgtcgg
     9121 tgaaacttcg tcgatgtcgc ctcattcata cctcctgtcg gtaaatctga caccctgacc
     9181 aatagcccag gctgttgtgt actcgatcag acttgccatg cgcttcacgc tcatctgcgc
     9241 gctgctttcg cgaatgttga cgcattctcc ttcaaggccg ggcaaaacat cagcttcctg
     9301 ttttgttgcc actgcatgac cgctgatcaa caaaaccttc cattgttctg gttttaacca
     9361 tttgccgcac cattgaacct gacgagcgat atccgccagc atcgcgtgaa attttgcgtt
     9421 ctggtcaaga tttcgcttgt agtcagtaat gcggatggtg actggcttgt ctttatcgag
     9481 aggagttgag aggatggcat ttattgcggc ttgctgttgt tgcttagttc ggaggaagat
     9541 tgtttgcttc atcgaaaatt cttctcttta attccagcgg ctctgatagc tttcattact
     9601 gcaattaccg ttttgtcctt cccatcctca tgccccatcg cataagcacc ttcttcacca
     9661 tctttccaaa agtcgtcatt cgattcgggc cagtcgatat ccagttcaat agctgctcgc
     9721 gatgcctgcc atatcaccca ggcaaactct tttaattcat cgtctcctgt gaactggctt
     9781 ttgtcttttg accaccagtt ttcaaactgt cggtagctat cgttcacttc cctctccccc
     9841 aaataaaaag gcctgcgatt accagcaggc ctgctattag ctcagtgatg tagatggtca
     9901 tttaatactc cgtcacgttt tcctgtcgcc acgcctcgtc atattccgat ttcggcatat
     9961 tggcgatgta gctatatggc gatcctgatt caagttgcag gaactggtgc gattgctcgt
    10021 caaggaacaa cggaacacca ccttcccaac cttcgccgtt acgttgtttt tcaagcatca
    10081 aaacagatgc
//
LOCUS       DAEEUE010000125        10059 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.125, whole genome shotgun sequence.
ACCESSION   DAEEUE010000125 DAEEUE010000000
VERSION     DAEEUE010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10059
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.125"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..60)
                     /locus_tag="KLK84_003523"
     CDS             complement(<1..60)
                     /locus_tag="KLK84_003523"
                     /inference="COORDINATES: protein motif:HMM:NF015750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010704.1"
                     /translation="MASISIRCPSCSATEGVVRN"
     gene            171..1661
                     /locus_tag="KLK84_003524"
     CDS             171..1661
                     /locus_tag="KLK84_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HBE2010705.1"
                     /translation="MDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKS
                     TPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETF
                     LPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDY
                     FMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAY
                     NAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTII
                     LFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDF
                     YPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWD
                     NYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDN
                     LAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1713..2870
                     /locus_tag="KLK84_003525"
     CDS             1713..2870
                     /locus_tag="KLK84_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HBE2010706.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2836..2958)
                     /locus_tag="KLK84_003526"
     CDS             complement(2836..2958)
                     /locus_tag="KLK84_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010707.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3161..4846
                     /locus_tag="KLK84_003527"
                     /pseudo
     CDS             3161..4846
                     /locus_tag="KLK84_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4884..7244
                     /locus_tag="KLK84_003528"
     CDS             4884..7244
                     /locus_tag="KLK84_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HBE2010708.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7301..>10059
                     /locus_tag="KLK84_003529"
     CDS             7301..>10059
                     /locus_tag="KLK84_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBE2010709.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNELGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYISYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNT"
BASE COUNT         2958 a         2235 c         2207 g         2659 t
ORIGIN      
        1 gttacgcacc acgccttcag tagcggagca ggaaggacat ctgatggaaa tggaagccac
       61 gcaagcacct taaaatcacc atcatacact aaatcagtaa gttggcagca ttacccttta
      121 cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc
      181 ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc
      241 gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat
      301 caacgccgac gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg
      361 cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct
      421 ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc
      481 ctgaattatt ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa
      541 aaatcagtaa tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca
      601 ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat
      661 tccacgctgc aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg
      721 tccccgcaaa aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc
      781 gtgccaaaac acttgaccag ccttttatgc tttacctggc ttataatgct ccgcacctac
      841 caaatgataa tcctgcaccg gatcaatatc agaagcaatt taataccggt agtcaaacag
      901 cagataacta ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac
      961 aactgaagaa aaacggacag tatgacaata caattattct ctttacctcc gataatggtg
     1021 cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct
     1081 atcctggcgg tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta
     1141 attatgacaa gctgatttcc gcaatggatt tctacccaac agctcttgat gcagccgata
     1201 tcagcattcc aaaagacctt aagctggatg gtgttccctt gctgccctgg ttgcaagata
     1261 agaaacaagg cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg
     1321 atgaggaaaa tattccattc tgggataatt accacaaatt tgttcgccat cagtcagacg
     1381 attacccgca taaccccaac actgaggact taagccaatt ctcttatacg gtgagaaata
     1441 acgattattc gcttgtctat acagtagaaa acaatcagtt aggtctctac aaactgacgg
     1501 atctacagca aaaagataac cttgccgccg ccaatccgca ggtcgttaaa gagatgcaag
     1561 gcgtggtaag agagtttatc gacagcagcc agccaccgct tagcgaggta aatcaggaga
     1621 agtttaacaa tatcaagaaa gcactaagcg aagcgaaata actaaacctt catgcggcgg
     1681 atttttccgc cgccttattg agcgagatag cgatgcacgt tacagccaag ccctccagtt
     1741 ttcaatgtaa tctcaaatgt gattactgtt tttaccttga aaaagagtcg cagtttactc
     1801 atgaaaaatg gatggatgac agcactctga aagagttcat caaacaatat atcgaagcgt
     1861 ctggcaatca ggtctatttt acctggcaag gcggtgaacc cactctggct ggcctggatt
     1921 ttttccgtaa agttattcac tatcaacaac gctatgcagg ccaaaaacgt atttttaatg
     1981 cattacaaac gaatggcatt ttattgaata atgaatggtg tgcctttctc aaagaacatg
     2041 aatttctggt tggtatctcg atcgatggcc cccaggagtt acatgaccgt tacagacgca
     2101 gtaattcagg taacggtact tttgcaaaag tgatagcagc catcgagcgt ctgaaatcat
     2161 atcaagtaga gtttaatacg ttaaccgtca ttaataacgt taatgtccat tatcctcttg
     2221 aggtttatca ttttttaaaa tctatcggca gtaaacatat gcaatttatc gaattgctag
     2281 aaaccgggac gccgaatatt gatttcagtg gtcatagtga gaacacattc cgtatcattg
     2341 atttttctgt gcctcccacg gcttatggca agtttatgtc aaccattttt atgcaatggg
     2401 ttaaaaacga tgtgggtgaa attttcattc gtcagtttga aagctttgtc agccgttttt
     2461 tggggaatgg gcatacaagt tgtattttcc aggagtcctg caaggataat ctggttgttg
     2521 aaagtaatgg agacatttac gaatgcgacc attttgtcta tccacagtac aaaattggaa
     2581 acattaataa atctgaactc aaaacgatga acagtgtaca actgacagcg caaaaaaaac
     2641 ggatttcagc gaaatgtcag caatgtgcat ataaacctat ctgcaatggc ggttgtccta
     2701 agcatcgtat tactaaagta aacaatgaga ctgtttctta tttttgcgaa ggttataaaa
     2761 tccttttttc aaccatggta ccttatatga acgccatggt tgagttagct aagaacagag
     2821 taccgcttta ccacattatg gatgttgcaa aacaaatgga gaataattaa taacgctctc
     2881 ttttaactcc attcttagcc tccttcttat gaccttaaca aggcacccga cataaattcc
     2941 tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac gtggtatgcg
     3001 tcagggcatt agcaataccc ttgattatca atagcataac cataaccact gaaaattaaa
     3061 aatttctata tatttcatat tgttaaatga tgcttaaata aatttcatca gtaaatcatt
     3121 gccatagata atcattggat ggcacaatca attttcgcta atgataacca ttcccattac
     3181 gttttgtatg ttgatagcaa agtatctctg tctgctgaag cccttttggc tgcgtaagaa
     3241 taataaaact tcagtgttgt taatcattat tatcctcgca atgatccttg gcgtggttaa
     3301 aattcaggtc tggttaaacg actggaataa tgattttttc aatgcgctaa gccagaaaga
     3361 aaccgataag ctttggcagc tcgttctttg gttccctgcc ctgctgggga tctttgtatt
     3421 aatatccgta aacaaaacat ggttaattaa actactgact attcgctggc gcgaatggtt
     3481 aacagattac taccttaatc gctggtttgc agataaaaac tactacttca cgcagatcta
     3541 cggcgagcat aaaaatacag ataaccctga ccaacgtatc gctgaagaca ttctcttact
     3601 gatcaacaaa acgctcagtc tttcatttgg cttcatccag tcacttagca tgctgatcac
     3661 ctttaccgtt attctttggc aaagcgcggg tacgctctca ttcactgttg gtggaacaga
     3721 atggaatatc caggggtata tggtctatac cgtcgtgctc attgtgatcg gtggaacttt
     3781 atttacccat aaggtgggta aacgtattcg tccgcttaat gtggaaaaac aacgtagcga
     3841 agcgacattt cgaactaatc ttgtgcagca taataagcag gcagaactga ttgcgctaag
     3901 caatgctgag tctctacaac gccaggagtt gcgcgacaat tttcatacca ttaaagagaa
     3961 ctggcatcgt ttaatgaatc gtcagcggtg gcttgattac tggcaaaata tttattcgcg
     4021 ctcgcttagc gttcttccct actttctgtt attgccgcag tttattagcg ggcagatcaa
     4081 tctgggcgga ctgatgaaat cgcgccaggc atttatgcta gtatcgaaca atttaagctg
     4141 gtttatttat aaatatgacg aacttgctga actggctgcg gttatcgatc gcttgtatga
     4201 gttccatcaa ctcactgaat agcgccctac gaataagcct aaaaattgcc aacatgcggt
     4261 acaagtggct aatgcgagta ttcgtacgcc tgataataag atcatattag agaacttgaa
     4321 ctttcatgtt tcgccaggca aatggctatt actgaaaggc tactctggtg cgggaaaaac
     4381 cacactgctt aaaacattat cccactgctg gccgtggttt aaaggtgata tttcttctcc
     4441 tgctgacagt tggtatgtgt cacaaacacc gttaatcaaa agcggcttac tgaaagagat
     4501 tatttgtaaa gcacttcccc tgcccgtaga cgataaatcg ttgagcgaag tactgcatca
     4561 ggttggtctg ggaaaattgg ctgcgcgtat tcatgaccac gatcgctggg gagatattct
     4621 ttccagcggc gaaaaacaac gtatcgccct ggcacgatta attttacgac gtccgaaatg
     4681 gatatttctt gacgaaacta cctctcatct tgaggaacaa gaggctatac gcttactgcg
     4741 tttagtgcgt gaaaaactac ccacaagcgg cgtcattatg gttacacatc aacccggtgt
     4801 ctggaacctg gctgatgata tttgtgacat tagcacggtt ttataacaac aataaaagcc
     4861 ctgctgtacg gagatataaa taaatgaagc gagttcttat tcctggcgtc attttatgtg
     4921 gcgctgatgt ggcgcaggcc gtcgatgaca aaaacatgta catgtatttt tttgaagaga
     4981 tgacggtcta tgctcctgtc cctgtacccg taaacggcaa cacgcattac accagtgaaa
     5041 gcatcgagcg tttaccgacc gggaatggca atatcagcga tctgctgaga accaaccctg
     5101 cggtacgcat ggattcaacg caaagtacct cgttgaacca gggagatatt cgccctgaga
     5161 aaatctctat tcacggtgcg tcgccctacc agaatgccta tttgattgac ggtattagtg
     5221 caactaataa cctgaaccca gcgaatgagt ccgatgccag tagtgcaacc aatattagcg
     5281 ggatgtcaca ggggtattat cttgatgtca gcttactgga caatgtgacg ctttatgaca
     5341 gttttgtgcc ggttgaattt ggtcgcttca atggcggggt aattgatgca aagatcaaac
     5401 gcttcaacgc tgatgatagc aaggtgaaat tgggttatcg cactacgcgt ttggactggt
     5461 taacatcgca tatcgatgag aataacaaga gcgcatttaa tcaaggttct tcaggaagta
     5521 cctatttctc tccagatttt aaaaagaact tttatacctt gtcgtttaat caggagctgg
     5581 ctgataactt tggcgttacc gccggtttat cgcgccgcca gtctgatatc acccgcgcgg
     5641 attatgtttc gaatgacggc attgtcgccg gtcgggcaca gtataaaaac gttatcgata
     5701 ctgcattgag caaatttacc tggtttgcca gcgaccgctt tacccacgat ttaaccttaa
     5761 aatataccgg ctccagccgt gattataata ccagcacctt cccgcagtct gatcgcgaaa
     5821 tgggtaataa atcctatggt ctggcatggg atatggatac gcaactcgca tgggccaaac
     5881 tacgtaccac cgttggttgg gatcatatta gtgattatac ccgtcacgat catgacatct
     5941 ggtacaccga actttcatgt acatatggtg atattacagg gcgttgtacc cgtggcggat
     6001 taggacacat ttcccaggct gtagataatt acaccttcaa aacacgcctg gactggcaaa
     6061 aattcgccgt gggtgatgtt tcgcatcaac cctacttcgg cgcggaatac atctattccg
     6121 atgcatggac tgaacgccat aaccagtctg aatcctatgt gattaatgct gcaggaaaga
     6181 aaactaacca taccatttac cataaaggta aaggcagcct gggaattgac aactacacgc
     6241 tgtatatggc ggatcacatt agctggcgga atgtgtcgtt aatgcccggt gtgcgttatg
     6301 actatgacaa ctatctgtca aaccacaata tctccccgcg ctttatgacg gaatgggata
     6361 tttttgctga tcaaacctca atgattaccg ccggttataa ccgttactat ggcgggaata
     6421 ttcttgatat gggattacgt gatatccgca atagctggac ggaatcggta tcaggtaata
     6481 aaaccctgac gcgttatcag aatttgaaaa cgccttataa cgatgaactg gcaatgggat
     6541 tgcagcaaaa aatcgataag aacgttattg cacgcgcaag tgaagcgcat gatcaaatca
     6601 gcaaaagcag tcgtaccgac agcgcgacta aaaccaccat tactgaatat aacaacgatg
     6661 gcaaaaccaa aacgcattcg tttaacctca gttttgaact ggccgaaccc ctgcatatcc
     6721 gccaggtaga tattaacccg caaattgtct ttagctatat caagagcaag ggcaacttgt
     6781 cgttaaacaa tggttatgag gagagcaata ccggtgataa ccaggtggtt tataacggta
     6841 atctggtctc ttacgatagc gttccagtgg cagattttaa taacccatta aagatctcct
     6901 taaacatgga tttcacgcat caaccgagcg ggttagtgtg ggcgaatacg ctggcctggc
     6961 aagaagcgcg taaagctcgc attatccttg gtaagacaaa tgcgcaatac atcagcgaat
     7021 attcagatta caagcagtat gttgacgaaa aactggatag cagcctgacc tgggacaccc
     7081 gcttgtcctg gacgccacaa tttctgaaac aacaaaacct gacgatcagt gccgatattc
     7141 tcaatgtact ggatagcaaa accgctgttg atacaacgaa caccggtgtg gcgacctacg
     7201 ccagtggccg tactttctgg cttgatgtca gcatgaaatt ttaatagttc ggattttact
     7261 taaccgggta aattcgcccg gttttcgcat ggagataatt atgagaaacc tctgtttctt
     7321 actgacgtta gtggcaactc tgttgctccc cgggcggctg attgccgccg ccttaccgca
     7381 ggatgaaaag ttaattaccg ggcaactgga caatgacttg cgatatatga tttatccgca
     7441 tgctcatcca aaggatcagg taaatttatg gctgcaaatt cataccggtt cattgcagga
     7501 agaagacaat gagctcggcg tggctcattt tgtagaacat atgatgttta acggcacaaa
     7561 aacatggccg ggtaataaag tcatcgaaac atttgagtca atgggcctgc gttttggtcg
     7621 cgatgttaat gcctatatca gctatgacga aacggtgtat caggtgagtt tgccgactac
     7681 gcagaaacaa aatctgcaac aagtgatggc aatcttcagt gaatggagta atgccgcaac
     7741 ctttgaaaaa ctcgaagtag acgctgaacg tggcgtaatt actgaggaat ggcgtgccca
     7801 tcaggatgcg aaatggcgca cctctcaggc gcgccgccct ttcctgctgg ctaatacccg
     7861 taatttagac cgtgaaccta tcggcctgat ggatactgtc gccacggtca cgccggcaca
     7921 attgcgccaa ttttatcaac gctggtatca accaaataat atgaccttta tcgtggtcgg
     7981 cgatatcgac agtaaagaag cgctggcgct gataaaggat aatttaagta agcttccggc
     8041 taacaaagca gctgaaaatc gcgtctggcc gacaaaagcc gaaaaccacc tgcgctttaa
     8101 tatcattaat gataaagaaa accgggtgaa cggcatcgca ctctattatc gcctgccaat
     8161 ggtacaagtg aacgatgagc aaagctttgt cgaacaagct gaatggagca tgttagttca
     8221 gctgttcaat caacgtctgc aggaacgcat acagtcgggc gagttgaaga ctatttctgg
     8281 cggcactgcg cgcagcgtta aaattgcacc cgattatcag tcgctgtttt tccgtgtaaa
     8341 tgcacgagac gataatatgc aggatgctgc gaatgcatta atggcagagt tggcaaccat
     8401 tgatcagcat ggtttttctg ctgaagaact cgatgatgtc aaatctaccc gcctcacctg
     8461 gctgaaaaat gcggttgatc agcaagctga acgtgattta cgtatgctga ccagtcgcct
     8521 ggcatccagc tcattaaata atacgccgtt cttgtcgccg gaagagacat atcaactttc
     8581 gaaacgtctg tggcagcaaa ttaccgtgca aagtctggcg gaaaaatggc agcagttaag
     8641 aaagaaccag gacgcatttt gggagcaaat ggtaaacaat gagcttgccg ccaaaaaagc
     8701 attgtctcct gcggctatcc tggcgctgga aaaagagtac gccaacaaaa agctggcggc
     8761 ttacatcttc ccaggcagaa atttatcgtt aacagtagac gctgacccac aggcggaaat
     8821 tagcagcaaa gaaacgctgg ctgagaatct gacatcatta acactttcca atggtgccaa
     8881 ggttattctg gcaaaatccg cgggtgaaga gcaaaagcta caaattattg ccgtatcgaa
     8941 taaaggcgat ttaagtttcc ctgcgcagca aaaatcactt atcgcgctgg caaataaagc
     9001 agttagcgga agcggcgttg gcgaactctc ctcttccagc ctgaaacgct ggagtgcgga
     9061 aaactcggta accatgagca gtaaagtcag tggcatgaat acgttgctct ccgttagcgc
     9121 gcggactaat aaccctgaac ctggtttcca gttgattaac cagcgaatca cccacagcac
     9181 gattaacgac aatatttggg catcgctaca aaatgctcaa attcaggcgt tgaaaacgct
     9241 cgaccagcgt ccagcggaga aattcgccca gctgatgtat gagacgcgct atgctgatga
     9301 ccgcacgaaa ttactgcaag aaaatcagat tgcacagttt actgccgcag atgcgctggc
     9361 tgccgatcgc cagttgtttt catctccagc ggatatcacg tttgtcattg tcggtaatgt
     9421 cgcagaagac aaactcgtgg cgttaattac gcgttactta ggctcaatca aacactctga
     9481 ttcgccatta gccgcaggta aaccattaac tcgcgcgacg gacaacgcat cggttactgt
     9541 aaaagaacaa aatgaacctg tggcacaggt ttcacagtgg aagcgttatg attcccggac
     9601 acctgttaat ctggcgacgc gtatggcgct cgatgctttt aacgtcgcac tggcaaaaga
     9661 tctacgcgtt aatattcgtg aacaggcatc tggggcatac agcgtttctt ctcgcctctc
     9721 ggttgatcct caggctaaag atatcagtca tttgctggct tttacttgtc aaccagaacg
     9781 acatgatgaa cagttaacgt tagcgaatga agtgatggtt aagcgtttga ctaaggggat
     9841 cagtgagcaa gaactgaacg aataccagca aaacgttcag cgcagcctcg atatccaaca
     9901 gcgtagcgtt caacaattag cgaacactat tataaatagt cttattcaat atgacgatcc
     9961 tgcagcatgg actgagcagg agcaattgtt gaaacaaatg acggtagaga atgttaacac
    10021 tgccgttaaa caatatcttt ctcatccggt aaatactta
//
LOCUS       DAEEUE010000126         9954 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.126, whole genome shotgun sequence.
ACCESSION   DAEEUE010000126 DAEEUE010000000
VERSION     DAEEUE010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9954
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.126"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            447..1505
                     /gene="citC"
                     /locus_tag="KLK84_003530"
     CDS             447..1505
                     /gene="citC"
                     /locus_tag="KLK84_003530"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HBE2010710.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISKSVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1520..1816
                     /gene="citD"
                     /locus_tag="KLK84_003531"
     CDS             1520..1816
                     /gene="citD"
                     /locus_tag="KLK84_003531"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HBE2010711.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1813..2721
                     /gene="citE"
                     /locus_tag="KLK84_003532"
     CDS             1813..2721
                     /gene="citE"
                     /locus_tag="KLK84_003532"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HBE2010712.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2732..4264
                     /gene="citF"
                     /locus_tag="KLK84_003533"
     CDS             2732..4264
                     /gene="citF"
                     /locus_tag="KLK84_003533"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HBE2010713.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMNVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAV
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4268..4819
                     /gene="citX"
                     /locus_tag="KLK84_003534"
     CDS             4268..4819
                     /gene="citX"
                     /locus_tag="KLK84_003534"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HBE2010714.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHRVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4794..5672
                     /gene="citG"
                     /locus_tag="KLK84_003535"
     CDS             4794..5672
                     /gene="citG"
                     /locus_tag="KLK84_003535"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HBE2010715.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5723..7185
                     /gene="citT"
                     /locus_tag="KLK84_003536"
                     /pseudo
     CDS             5723..7185
                     /gene="citT"
                     /locus_tag="KLK84_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7299..8105
                     /gene="rna"
                     /locus_tag="KLK84_003537"
     CDS             7299..8105
                     /gene="rna"
                     /locus_tag="KLK84_003537"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HBE2010716.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8334..8744
                     /gene="rnk"
                     /locus_tag="KLK84_003538"
     CDS             8334..8744
                     /gene="rnk"
                     /locus_tag="KLK84_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HBE2010717.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8975..>9954)
                     /locus_tag="KLK84_003539"
     CDS             complement(8975..>9954)
                     /locus_tag="KLK84_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBE2010718.1"
                     /translation="IACGDCFFCRLQQYAACENTNAGKGAALNKKQIPAPAALFGYSH
                     LYGGVPGGQAEYVRVPKGNVGPFKVPPLLSDDKALFLSDILPTAWQAAKNVQIQQGSS
                     VAVYGAGPVGLLTIACARLLGAEQIFVVDHHPYRLRFAADRYGAIPINFDEDSDPAQS
                     IIEQTAGHRGVDAVIDAVGFEAKGSTTETVLTNLKLEGSSGKALRQCIAAVRRGGIVS
                     VPGVYAGFIHGFLFGDAFDKGLTFKMGQTHVHAWLGELLPLIEKGLLKPEEIVTHYMP
                     FEEAARGYEIFEKREEECRKVILVPGAQSAEAAQKAVSGLVNAMPGGTI"
BASE COUNT         2351 a         2677 c         2507 g         2419 t
ORIGIN      
        1 agatacgcag cggaaatgcc agtctttgga aaaatgcaaa ctgtttattc tcgttaagct
       61 gcaacattgt tttccttgcg ggttgtcccg agcctctttg acggatgtaa ttaaaatgtt
      121 gaatcaaaat acatagatat taattatgat cgtggcaaat tttattcatt aaatcaatta
      181 aatcacttaa acctgcgagt catttcgtaa ttacattaat taaaaaactt aaaaccatta
      241 aataaataaa aaaccactaa ctctatgtga aataaatcaa aatttcacgc cgaaatactc
      301 cttaggatgt atagcgaaag gagaaaaaga tatacctcga tcacccccct ttctcccaag
      361 tgaaaataaa aggttatcag tttgcaacat tgaacaacat tcgttgcaaa tcgataacaa
      421 catgcacctt caggatacta tttattatgt tcggcaatga tattttcacc cgcgtaaaac
      481 gttcagaaaa taaaaaaatg gcggaaatcg cccaattcct gcatgaaaat gatttgagcg
      541 ttgacaccac agtcgaagta tttattaccg taacccgcga tgaaaagctt atcgcgtgcg
      601 gtggaattgc cggaaatatt attaaatgcg ttgctatcag taaatccgtt cgcggtgaag
      661 gactggcgct gacattagcc actgaactga taaacctcgc ctatgagcgg cacagcacgc
      721 atctgtttat ttataccaaa accgaatacg aggcgctgtt ccgccagtgc ggtttttcca
      781 cgctgacctg cgtacccggc gtgatggtgc tgatggaaaa cagcgccacg cgactgaaac
      841 gctatgccga atcgctgaaa aaatttcgtc atccagggaa caagattggc tgcattgtga
      901 tgaacgccaa tccctttacg aatggtcacc gttatctgat tcaacaagct gcggcacagt
      961 gcgactggtt gcatctgttt ttagtcaaag aagattcttc acgcttcccc tatgaagacc
     1021 ggctggatct ggtgttaaaa ggcaccgccg atattccacg cctgactgtg catcgcggct
     1081 cagaatacat catctcccgc gctacgttcc cttgctactt cattaaagaa cagagcgtca
     1141 ttaaccattg ttacaccgaa attgatctga agattttccg tcagtacctc gctcccgcgc
     1201 ttggtgtaac tcaccgcttt gtcggtactg aacccttttg tcgcgttacc gcccagtaca
     1261 accaggatat gcgctactgg ctggaaacgc cgactatctc cgcagcgccc atcgaactgg
     1321 ttgaaattga gcggctgcgt taccaggaga tgccgatatc cgcttcccgg gtacgtcaac
     1381 tgctggcgaa aaacgatctc acggctatcg cgccgctggt ccctgcagtc acgctgcatt
     1441 atttgcagaa cctgcttgag cactcccgcc aggacgcggc agctcgtcaa aagacccccg
     1501 catgagaaac aggtgaaaaa tgaaaataaa ccagcccgcc gttgcaggca cccttgagtc
     1561 tggggatgtg atgatacgca tcgccccact cgatacgcag gatatcgacc tgcaaatcaa
     1621 tagcagcgtt gagaaacagt ttggcgatgc aattcgcacc accattctgg acgttctcgc
     1681 ccgctacaac gtgcgcggcg tacagctgaa tgtcgatgac aaaggcgcac tggactgcat
     1741 tttacatgca cgactggaag ccctgctggc acgcgccagc ggtatcccgg ctctgccatg
     1801 ggaggattgc caatgatttc cgcttcgctg caacaacgta aaactcgcac ccgccgcagc
     1861 atgttgtttg tgcctggtgc caatgccgcg atggtcagca actccttcat ctacccggct
     1921 gatgccctga tgtttgacct cgaagactcc gtggcattac gtgaaaaaga caccgcccgc
     1981 cgcatggttt atcacgcgct gcaacatccg ctgtatcgcg atattgaaac cattgtgcgt
     2041 gtcaacgcgc tggattccga atggggtgtt aacgacctgg aagccgtcgt tcgcggtggt
     2101 gcggacgttg tgcgtctgcc gaaaaccgat accgctcagg atgttctgga tattgaaaaa
     2161 gagatcctgc gtatagaaaa agcctgtggt cgtgaacccg gcagcaccgg cctgctggcg
     2221 gcgattgaat ctccgctggg cattacccgc gcagtggaaa tcgctcacgc ttccgagcgt
     2281 ttgatcggta tcgccctcgg tgcagaagac tatgtgcgca acctgcgtac agaacgctcc
     2341 ccggaaggaa ctgaactgct gttcgcacgc tgttccattt tgcaggccgc gcgctctgcg
     2401 ggtattcagg cgttcgatac cgtctattcc gacgctaaca acgaagccgg attcctgcaa
     2461 gaagccgccc acatcaaaca gctgggcttt gacggcaaat cgctgatcaa cccgcgtcag
     2521 attgatctgc tgcacaacct ctacgcaccg acccagaaag aagtggatca cgcccaccgc
     2581 gtcgtagaag ccgctgaagc cgccgctcgc gaaggcctcg gcgtggtttc cctgaacggc
     2641 aagatggtgg acggtccggt tatcgatcgc gcccgtctgg tgctctcccg tgcagaactt
     2701 tccggcatcc gcgaagaata aggcaatcaa aatgacgcag aaaattgaac aatctcaacg
     2761 acaagaacgg gtagcggcct ggaatcgtcg cactgaatgc gatcttgccg ctttccagaa
     2821 ctcaccaaag caaacctacc aggctgaaaa agcgcgcgat cgcaaactgt gcgccaacct
     2881 ggaagaagcg attcgtcgct caggtttaca ggacggcatg acggtttcct tccatcacgc
     2941 tttccgtggc ggtgacctga ccgtcaatat ggtgatgaac gtcatcgcga agatgggctt
     3001 taaaaacctg accctggcgt ccagctccct gagtgattgc catgcgccgc tggtagaaca
     3061 cattcgccag ggcgtggtta cccgcattta tacctctggc ctacgcggtc cactggcgga
     3121 agagatctcc cgtggtctgc tggcggaacc ggtgcagatc cactctcacg gcggtcgtgt
     3181 gcatctggta cagagcggcg aactgaatat cgacgtggct ttcctcggcg tcccgtcctg
     3241 tgatgaattc ggtaatgcca acggctacac cggtaaagcc tgctgcggct ccctcggcta
     3301 tgcaatggtt gatgccgaca acgcaaaaca ggtcgtgatg cttaccgaag aactgctgcc
     3361 ttatccgcat aatccggcaa gcattgagca agatcaggtt gatttgatcg tcaaagttaa
     3421 tcgcgttggc gatgctgcaa aaatcggcgc tggtactacc cgtatgacca ctaacccgcg
     3481 cgaactgctt attgcccgta gcgctgcgga tgtgattgtc aactctggct acttcaaaga
     3541 aggtttctcc atgcaaaccg gcaccggcgg cgcttcgctg gcggtaaccc gtttcctgga
     3601 agacaaaatg cgtagccgcg atattcgcgc cgacttcgcc ctcggcggta ttaccgcgac
     3661 gatggttgat ctgcacgaaa aaggtctgat ccgcaaactg ctggatgtgc agagctttga
     3721 cagccatgct gtgcaatcgc tggcccgtaa ccccaatcac atcgaaatca gcgccaacca
     3781 gtacgctaac tggggttcga aaggcgcatc ggttgatcgt ctcgacgtgg tggtactgag
     3841 cgcgctggaa attgacaggc agttcaacgt taacgtgctg accggttctg acggcgtact
     3901 gcgtggcgct tccggtggtc actgcgatac cgcgattgcc tctgcgcttt ccatcatcgt
     3961 cgcgccactg gtacgcggtc gtattccgac tctggtggat aacgtgctga cctgcatcac
     4021 cccgggctcc agtgtcgata ttctggtcac agaccacggt atcgcagtta acccggcacg
     4081 tccggaacta gcagaacgtc tgcaggaagc gggcattaaa gtggtttcca ttgagtggct
     4141 gcgcgaacgt gcgcgtctgc tgaccggtga accacagccg attgaattca ccgaccgcgt
     4201 cgttgccgtt gtgcgttacc gcgatggctc ggtgatcgat gttgtgcatc aggtgaagga
     4261 ataagccatg cacctgcttc ctgaactcgc cagccaccat gcggtgtcaa ttcccgagct
     4321 gctcgtcagc cgggatgaaa ggcaagcacg gcaacacgcc tggctcaagc accatcctgt
     4381 tccactggtc tcctttaccg tggttgcgcc tgggccgatt aaagacagcg aggtcacacg
     4441 ccgaattttt aatcatcgcg tgacagcctt gcgtgcctta gctacaaagc agggctggca
     4501 aattcaggag caggctgcac tggtttccgc cagcgggccg gagggcatgt tgagcattgc
     4561 cgccccggct cgcgacctca agctcgccac cattgagctt gaacatagtc atcctctcgg
     4621 gcggttatgg gatatcgatg tcctgacgcc cgaaggcgaa attctctccc gccgcgacta
     4681 ttcactgccg cctcgccgct gcctgttgtg cgagcaaagc gcagccgtct gcgcgcgtgg
     4741 aaaaacccat caactgaccg atttactcaa ccgcatggag gcactgctga acgatgtcga
     4801 tgcctgcaac gtcaactaaa accacaaagc ttgcgacgtc attaatcgat gagtacgccc
     4861 tgctgggctg gcgcgccatg ctgactgaag tcaatctgtc accgaaacca ggcctcgtgg
     4921 atcgcattaa ctgcggtgcg cacaaagata tggcgctgga agatttccac cgcagcgcgc
     4981 tggcgattca gggctggtta ccccgtttca ttgaatttgg tgcctgtagt gcagaaatgg
     5041 caccggaagc ggtactccac ggattacgcc caattggtat ggcttgcgaa ggtgatatgt
     5101 tccgcgccac tgcgggcgta aacacgcata aaggcagcat tttttcttta gggctgctat
     5161 gtgcggcaat tggccgtttg cttcagctca accagccggt tacgccaaca accgtttgtt
     5221 ctacggcggc aagtttctgc cgtggcctga ccgatcgcga actgcgtacc aataattcac
     5281 gactgacggc aggtcaacgg ttgtaccagc agcttggcct taccggcgca cgcggtgaag
     5341 ccgaagcagg ttatccactg gtgatcaacc acgccttgcc gcattacctc actctgctgg
     5401 atcaggggtt agatcctgaa ctggcattgc tcgatacctt gctcctactg atggctacca
     5461 acggcgatac caacgttgca tcgcgcggtg gcgagggggg cctgcgctgg ctacagcgcg
     5521 aggcgcaaac attattgcaa aaagggggca ttcgaacccc caccgatctc gattatctcc
     5581 ggcagttcga cagggagtgt atcgaacgaa atctcagtcc aggcggcagt gccgacctgc
     5641 tgatccttac ctggttttta gcacagattt aattatttaa gcacttgata aatttggaaa
     5701 tattaatttt cggagaaccc gtatgtcttt agcaaaagat aatatatgga aactattggc
     5761 cccactggtg gtgatgggtg tcatgtttct tatccctgtc cccgacggta tgccgccgca
     5821 ggcatggcat tacttcgctg tgtttgtggc aatgattgtc ggcatgatcc tcgagccaat
     5881 tccggcaaca gcgatcagtt ttattgcggt tactatttgc gttattggca gtaattacct
     5941 gctctttgat gccaaagaat tagctgaccc agcgtttaat gcgcaaaaac aggcgctgaa
     6001 atggggcctg gctggttttt ccagcaccac ggtatggctg gtatttggcg catttatttt
     6061 tgcattaggg tatgaagttt ccgggttagg tcgtcgcatt gcccttttcc tggtgaaatt
     6121 catggacaaa cgcacgctga cgttgggtta tgcgattgtc attatcgaca ttctgctggc
     6181 accgtttaca ccgtccaaca ccgcgcgtac cgggggtacg gtttttccgg tcattaaaaa
     6241 cctgccgccg ttgtttaaat cattcccgaa cgatccgtcc gcgcgtcgta ttggcggcta
     6301 tttgatgtgg atgatggtca ttagtaccag tctgagttcg tccatgtttg tcaccggtgc
     6361 ggaccaaacg tgctgggtct ggagttcgtc agcaaaattg ccggtatcca gattagctgg
     6421 ttgcagtggt tcctctgctt cctgccggtt ggggttatct tgcttatcat tgcgccgtgg
     6481 ctttcctacg tgctgtacaa accggaaatc acacacagtg aagaagtggc aacctgggcg
     6541 ggtgatgaac taaaaaccat gggtgcgctg acacgcagag agtggacgct gattggcctt
     6601 gtattgctca gcttaggttt gtgggtattt ggcagtgaag tcattaatgc tactgcggtt
     6661 ggtctgctgg cagtttcgct aatgctggct ctgcacgttg tgccgtggaa agacattacc
     6721 cgctataaca gcgcatggaa cacgctggtc aacctggcaa ctctggttgt gatggctaac
     6781 ggcctgactc gttctggttt tattgactgg ttcgccggta ccatgagtac gcacctggaa
     6841 ggattctcac caaacgcaac ggtgattgta ctggttctgg tgttctactt tgcacactac
     6901 ctgtttgcca gcctgtctgc gcacaccgca accatgctgc cggttattct ggccgtcggt
     6961 aaaggtattc cgggcgtacc aatggaacaa ctgtgtatcc tgctggtgct gtctatcggt
     7021 atcatgggct gtctgacgcc gtatgcaacc ggtcctgggg tgattattta cggctgtggc
     7081 tatgtgaaat caaaagatta ctggcgtctt ggcgcaatct tcggggtgat ttacatctct
     7141 atgttgctgt tggttggctg gccgattctc gccatgtgga actaatacgt tcgccaaacg
     7201 ctgaataaat catcacgccc gccaggtaag cccacctggc gggctttttt atgatttaat
     7261 agatagtatt cagtgtatgt atgagttcca cacccattat gaaagcattc tgccgtaacg
     7321 ccgcgttgct cgcggtttct ctgcttccct tatcttctgc caacgccgta gcgttgcagg
     7381 caaaacagta tggcgatttt gatcgctatg tcctggccct ctcctggcaa accggatttt
     7441 gccagagtca acacgatcga aatcgtaacg aacgagatga atgtcgcctg caaaccgaaa
     7501 cgaccaacaa aactgatttt ctgaccgtac atggtctgtg gccaggattg cctaaatcgg
     7561 ttgctgcccg tggtgttgat gaacgccgct ggatgcgctt cggttgcgct actcgcccaa
     7621 tcccgaatct accagaagca cgcgccagcc gaatgtgttc atcgccggaa accgggttat
     7681 cactggaaac ggccgctaaa ctaagtgaag tcatgccagg agctggcgga cgttcctgcc
     7741 tggaacgcta cgaatatgcc aaacacggtg cctgctttgg ttttgatccg gacgcatact
     7801 tcggtacgat ggtacgcctg aatcaagaaa ttaaagagag cgaagccgga aaattccttg
     7861 cggataatta cggtaaaaca gtgagccgcc gtgactttga cgccgccttt gccaaaagct
     7921 ggggaaaaga gaacgtgaaa gcagttaagc taacgtgcca gggtaaccct gcgtatttga
     7981 ctgaaattca gatctcgatc aaagctgacg ccatcaacgc tccgctttct gcaaactcat
     8041 tcttgccaca acctcaccca ggtaactgtg gcaaaacctt tgtgattgat aaagcgggtt
     8101 attaatcctc tgatttacaa aacacgctaa ttcgtcttcc ataaccatgc cgattaagaa
     8161 ttatacataa tgcgctgcat tgaggaattc cctgaatgtg acgcaaatca cttcaaagag
     8221 tgagcaacag tcagtatcat catgtgagtt aaaccctcgc cgcctgacgg tgagggtttt
     8281 cttttgggta tgtttatctg cgacactcgc agtaccgacg acatggagta aaaatgtcca
     8341 gaccaactat catcattaac gacctggatg ccgaacgcat cgatattctg ctggagcaac
     8401 ccgcctatgc tggtttgcca atcgccgacg cgttaaacgc agagttggat cgcgcccaaa
     8461 tgtgttcgcc agaagagatg ccacacgacg tggtgacaat gaacagccgg gttaaattcc
     8521 gcaatcttag cgatggcgaa gtgcgtgtgc gcacgctggt gtatccggca aaaatgaccg
     8581 atagcaatac tcagctttcc gttatggctc cggtaggtgc cgcactgctg gggctgcgcg
     8641 ttggcgattc cattcactgg gaacttccgg gcggcgttgc aacccacctt gaagtgctgg
     8701 aactcgaata ccagccagaa gctgctggcg actacctgct ttaatcccat ctgcacaagc
     8761 gataactcgc tttgcccaga ggatgcactc gcatcctctt tacgcaaaat cagcaatttt
     8821 tcctggttct cactgttcac ttttgctgca aaattattcc gtttcgtttg tgggaataac
     8881 catggcagaa gcattttaca tcctgatagg atttctgatt atggcggcga ttatcgtcat
     8941 ggctgtcctt tacctcgaaa accactcctg acgatcatat tgttcccccc ggcatcgcat
     9001 tcaccagacc tgaaaccgcc ttctgcgccg cctctgcgct ttgtgcaccg ggtaccagaa
     9061 tcaccttacg gcactcctct tcacgttttt cgaaaatctc atatccccgg gcggcctctt
     9121 caaacggcat atagtgggta acaatttctt ctggtttcag taatcctttc tcaattaacg
     9181 gtaataattc tcccagccat gcgtgaacgt gggtctgtcc cattttaaac gtcaaccctt
     9241 tatcaaaggc gtcgccaaac aggaaaccgt gaataaatcc agcgtagacg cccggtacgc
     9301 taacaatgcc gccacgcctg accgccgcaa tacactggcg caacgcttta ccgctgctgc
     9361 cctccagttt caggttagtc agcaccgttt ccgtggtgct gcctttcgct tcaaaaccga
     9421 cggcgtctat tactgcatcc acgccccggt gacctgccgt ttgttcaata attgactgtg
     9481 ccggatcgct atcttcatcg aaattaatcg ggatcgcgcc gtagcggtca gcggcgaaac
     9541 gcaagcggta gggatgatga tcaacaacaa aaatctgttc cgcaccgagc aaccgtgcac
     9601 aggcgattgt caacaatccc acaggaccag caccatagac tgcaacgctt gaaccttgtt
     9661 ggatctgcac attttttgct gcctgccatg ccgttggcag aatatcagaa aggaaaaggg
     9721 ctttatcatc tgaaagcaaa ggtggtactt taaacggccc cacattccct ttcgggacgc
     9781 ggacatattc cgcctgcccg ccaggaacgc caccatacag gtgactataa ccaaacaatg
     9841 ccgcgggagc aggtatctgt tttttattga gcgcagcgcc tttacccgca ttggtatttt
     9901 cgcaggcggc atattgctgt aatcgacaga aaaaacagtc gccacaagca atga
//
LOCUS       DAEEUE010000127         9927 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.127, whole genome shotgun sequence.
ACCESSION   DAEEUE010000127 DAEEUE010000000
VERSION     DAEEUE010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9927
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.127"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..338
                     /locus_tag="KLK84_003540"
     CDS             <1..338
                     /locus_tag="KLK84_003540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010719.1"
                     /translation="SSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLNA
                     RNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYNI
                     DPLCECFKY"
     gene            526..1641
                     /locus_tag="KLK84_003541"
     CDS             526..1641
                     /locus_tag="KLK84_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010720.1"
                     /translation="MFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVGPDILAL
                     PVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFD
                     LRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRSVALAKT
                     VAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISVRNKYSP
                     AFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAKDRTISI
                     VLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKDLFDIRVLHVSNK
                     FNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            2320..2538
                     /locus_tag="KLK84_003542"
     CDS             2320..2538
                     /locus_tag="KLK84_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HBE2010721.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2756..2998
                     /locus_tag="KLK84_003543"
     CDS             2756..2998
                     /locus_tag="KLK84_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010722.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3328..4257)
                     /gene="fdhE"
                     /locus_tag="KLK84_003544"
     CDS             complement(3328..4257)
                     /gene="fdhE"
                     /locus_tag="KLK84_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HBE2010723.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4254..4889)
                     /gene="fdoI"
                     /locus_tag="KLK84_003545"
     CDS             complement(4254..4889)
                     /gene="fdoI"
                     /locus_tag="KLK84_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBE2010724.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4886..5788)
                     /gene="fdoH"
                     /locus_tag="KLK84_003546"
     CDS             complement(4886..5788)
                     /gene="fdoH"
                     /locus_tag="KLK84_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HBE2010725.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5801..8851)
                     /gene="fdnG"
                     /locus_tag="KLK84_003547"
     CDS             complement(5801..8851)
                     /gene="fdnG"
                     /locus_tag="KLK84_003547"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8264..8266),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBE2010726.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9045..9878
                     /gene="fdhD"
                     /locus_tag="KLK84_003548"
     CDS             9045..9878
                     /gene="fdhD"
                     /locus_tag="KLK84_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBE2010727.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2491 a         2396 c         2541 g         2499 t
ORIGIN      
        1 tgtcgagtaa taatgaatat gacctgttta aaaatagaga aacctcactg tggaaacgca
       61 aagtaccaga aagcgcagcg ctggcaatga cgggaacagt atataaatta tttggtaatc
      121 gcttactcaa cgcccgcaat cagttagata aaaatctcct cgaaatggct atgcaaatgg
      181 tttatggcaa agatatggtg acaaagtccg gggatatatt gattgatata caattaaata
      241 aggatggcag tctccagagt aagcactaca cacttgatgt aggtgaagtt ataaacagtt
      301 ataatattga ccctctttgt gaatgcttca agtattaatc aaacttactt acatgataaa
      361 aatcctggtg acgttattag cctgtatggc actttagata acattcccct tgtagttaag
      421 gaatgcacgg gtaaaaatca tcaaattaat gttaaagcca aattgccaca agaacgaagt
      481 gaaaaagttg aggatatgtg tgaaaaaatg aaaggtggca tctctatgtt taaccacacc
      541 acaaaaacag ctgataatat agagcataat ttgcagctaa gttttctcgt gggttcacgt
      601 ccgtcgatca ccaataaata ctcagcaaaa gatgtaggtc cagatatact tgcattgccc
      661 gtcaacgtat atcatgcgga ttttaaaatt aaaatcaata atgcagtgga gaaggcttca
      721 tcaattgtgt tatgtgctgt gcaaaaaaat ctccatgcag caaggaaggt tcttagcggg
      781 ataataggaa atgtgccaaa aaataataaa ccaaaaacaa ttatttttga tttgcgtttt
      841 cttagtgcaa ttaagcagaa agtttttttg ggcagtgaaa aaccgttgtt tgtaaagcat
      901 accgcaatga tgagggaggc atgtaaggaa ttaccgcagt cagtggaata tgttccttta
      961 gcctgtgaag cgcatagcca tcgcagcgtc gcccttgcca aaaccgtggc tgcgcccatc
     1021 atggcgacag gtgtgggctt ggcattaatg tatgcaacat catcaggacc gtattatgcg
     1081 caaagtcttt ctggttcgcc ggatatcgag atgataaaaa atcgcatggc tgaattgttt
     1141 aaccagtcgg atatttctgt gcgcaacaaa tatagcccgg cttttgataa atggaatgaa
     1201 ctggtagata aattaaatca cgaaagaaat acagttccgt ttgcgtgttt gaccaccatt
     1261 ttagcgacgg cgataaatga aactcttgag ggggatacga atgttgccgt tgtgatgggg
     1321 tgtaaatctg cgaaagatcg cacgatatcc attgtccttg gcaactcgat gttgcaaaca
     1381 ctccttgaaa aacgcctcgc cgatagtgga gaaatagaaa atctttttga tcaacaggga
     1441 tattttagtt gcgattcatt aacacccgaa gaattaatga tgttaaaaga cctttttgat
     1501 attcgagttc ttcatgtctc taacaaattc aatgttggat tgcaaggtaa tatcaacact
     1561 gatgttttac aagattcgtt ttttaaaaat gtcgatttca ttcatgaata taactcgtat
     1621 accgtaggga tgggagtctg acgagtaaaa ctcattagga ttatcagaat tcggacaatg
     1681 cattgcaaaa atgacataca aagagagatg tgtgcaggta cttagccgcg aggggcatgg
     1741 cgaagccata cagaaagtac cagtattcag aactccagtg aaagcggcat agtggccgcc
     1801 tgctcaggag ttttgctatg acccgtaatc gtgctgaacg tcgccaccct atgatgcgat
     1861 taaaaaaact catcgtcgcg ctgatgattg acgtcaatgc tatcggatga tgccgggggt
     1921 gttccggaag gaattccttg tttcccgaac tgttaccaac aaaattataa cgatgagcaa
     1981 gtaaagaccg gttggtggca gtactgaccg gtctgtgatg aaagattaat ttcaccttaa
     2041 atcctggtgc tgttaggatg ctgacgtttt tcggctacac cattcagatt atgcaaataa
     2101 atatccctga atataatata ttcatagcac ccataactca tcctgggcga taactaaacc
     2161 atttttgagt aataaatgaa acaactgcaa cgctcttatt atttctggaa aacgcctcgc
     2221 aaaaacaaaa tgacatgcaa aaatccgcat ttttttaaat gcttttccgg cgtaattttc
     2281 gcacatacat taatgagtaa tatatgtaag gtggtggtaa tggcgatgaa tacggttttt
     2341 cttcatttat cagaagaggc aattaaacgg ctgaacaagc ttcggggctg gcgtaaggtt
     2401 tcacgatctg caattttacg cgaagcggtt gagcaatatc tggagcggca gcaatttccg
     2461 gtgcgtaaag caaagggagg caggcaaaag ggggaggtag ttggtgttga tgatcagtgt
     2521 aaggagcata aggaatgaaa aaaatttttc attcttgaat ataaaaaaca gatgccctta
     2581 ttctggtatt aatacaaggc tgttttactt gaacttataa taactgcaac tgttacatcg
     2641 tatctggaaa acgcctcgca aaaataaaaa atgatgcgta aatgagcttt ctttaaagat
     2701 tcggcaaggg tacttttctc tccatagatt caaaaatgat attggcgaga tatttatgaa
     2761 ctctctggca ggtatagata tgggcagaat tttactcgat ttatcgaatg aggtgattaa
     2821 gcaacttgat gatctcgagg tgcagcgtaa tcttcctcgt gcggacctat taagggaagc
     2881 ggtagatcaa tacctgataa atcaatcgca aacagcaaga accagtgttc ctggcatctg
     2941 gcaagggtgt gaggaagatg gtgtcgaata tcagcgtaag ctgcgcgagg aatggtaatg
     3001 ggaaaaaatg atgtcaatca gattgctgac aacgtgcgcg ttgttcatgc cggatgcggc
     3061 gtgaacgcct tatccggcct acaaagtcat gcaaattcaa tatattgaag tttattggta
     3121 ggcctgataa gcgcagcgca tcaggcaatc atacgcttgt catcagtgac atacccataa
     3181 ccttatcgat gtgttggagg gaagaacgag gcaaacagac aattgcggca tgatcttgaa
     3241 tgggtaacgc ggaatacgag agatggtgtc gtcacaccct atgagctgta atccgggcga
     3301 cctgcgccac ccggaaaacg tcagagatta ctctccttca cccggaaaca ggaacgggtt
     3361 aatggaacta cgggcgtagc cttcttgctc cattcgcgcg tccagtacca gagaggcgag
     3421 gtcatctgcc acggcttcaa ctttcggttc tttttcctga taaagaatct tcaggtaagt
     3481 gccgcagtca tcgcagcttt cggctttaat cgcggcctgt tcgtcatcca gcgaccagta
     3541 atgcagtttg ccgctctgct cgcagttgct gcattttacg cgcactacgt gccattcggt
     3601 ttcacacagg ttgcagtgca ggtaacgcag accctgagtg gtgccaattt gcaccatgct
     3661 ggacaccggc atagagccac ataccgggca atattgacgt tgttcgccgt attcagcgcg
     3721 ggctttgccg gggatcagat tggccatctg cgcccagtag agcgacagcg cagcccagat
     3781 aaacggcgct ttatcgctgc tgacggacga gaaatcagag gcaaacagcg cgctggccat
     3841 atcttccagc tcctgagtcg atgccttctc cagattctca atcactgcca gcgccgggcc
     3901 gctcatttca ggtttcagct cagcaattag cgccatcagc agcttttgcc agtgcttatc
     3961 acgcggcaga acgtgaatat ccagcggggg cttgccctgt gcgctggctt ctttaatgcg
     4021 cgcggtcaga tccatctcca gcggatggtc gtacagcacc actttctggg cgtgggcgat
     4081 aagcgcagca aagcgcaggt aatcacccag cggattattt tctgccagct cgcgcagacg
     4141 ctcggcgcgg cggttgtata aattcttgag ccgagggaac aataacggcg gaatcatatc
     4201 cgccgtacgt ttctcgctcg aacccagctc atcttgcggg attatgcgaa tactcattca
     4261 gctttctttt ccgttgtctt gcggacctca cggtaccagc gcgggtgatg tttcttcgcc
     4321 caggcgctgg taacccatcc ttccaccatg gcggtaatcg tgcctttcac ccaaagggcg
     4381 gcgtagatat gcaccatgat aaccacaatt aacgccactg cggcaaatga atgcagcatt
     4441 aacgcgaatc ggatcaccgg gattgagaaa gcaggcgcaa aataaggacg ccagataatc
     4501 acgccgctca ccagcagcag aaccaggaaa ataatcgccg cccagaaaac gcatttctga
     4561 ccgaagttat aacgcccggt gtcacctact tcctcgttga cgacgatctt acgaatattc
     4621 ttcgcccaaa agatatcatc ccgattgatt aggttttgat gccagtaacg gaaaaacatg
     4681 atgatgaacg aggcaaacat aaccacgccg acaaacgggt gcagaattcg cgccagctgc
     4741 ggtgtgccca tgatttgcat caaccagttg aaggacggga acaaaaagcc cagcccgctc
     4801 accgccgcca ggatgaagca gaaggcggtg atccagtggt tgatacgttc cggcgcggtg
     4861 tagcgcacga tggtgtcacg tcgtttcatt tgcgctcctc gtctttctct tcgtgcagat
     4921 tattctcttc ctcatccgca cggttcggac cgacacccac gtagtggaag atactggccg
     4981 caaaggtagc cgcaaagcca acagccgcga gcggtttcca gatgcctttc cagaatttca
     5041 cggtttcgct gatttccggg ttctccggca agccatgata cagatttggc ttgtcagcat
     5101 ggtgcagcac gtacatgacg tgtgtaccac cgacgcctgc cggatcgtac agacccgcat
     5161 tgtcgtaacc gcgggttttc agctcagcaa cgcgctcgct cgccagcgtt ttcatcgact
     5221 ctttcgtgcc gaagtgaatc gcgcccgttg ggcaggtctt cacgcaggct ggttcttgcc
     5281 caaccaccac gcggtcaacg cacagcgtac atttgtagac gcggttgtct tccgggttga
     5341 ggcgcggaat gtcgaacgga cagcccgcaa tgcaataacc gcagccaatg cactgctcgg
     5401 actggaagtc gacgataccg ttggcatact gaataattgc accttccgcc gggcacgctt
     5461 tcaggcagcc tggatcggaa cagtgcatac agccgtcttt gcggatcagc cattccagtt
     5521 tgtcgttctg ctccacttcc gagaagcgca ttaccgtcca cgatttggcg cttaaatcat
     5581 tggggttgtc gtacacccca atgttattgc cgacggtatc gcgaatgtcg ttccactctg
     5641 aacaagccac ctgacaggct ttacagccga tacaggtggt aacgtcgatg agtttcgcca
     5701 cttcttcctg gaagtcccgc gcctgaggcg cgggggtcag accgttagtc gcggaacgac
     5761 gaatgatatc ttgagattga taagccatat gtcgtctccg ttacaccttt tccacattca
     5821 caaggaagga cttaaactcc ggcgtctgcg tgttcgcatc accaacgaat ggcgttaaag
     5881 tattggcaat aaagcctttt ttagctacgc cttcatagcc ccagtgaatc ggaataccga
     5941 tggtatcgat atctttgccg ttcgctttca gcgtgcgaat acgtttggtc accaccgctt
     6001 tggctttgat atagccacgg ttggaggaga ctttcacggt atcgccctgg gcaatgccaa
     6061 gtttattcgc cagcgactcc ccgatttcca caaactgctc tggctgcaaa atcgcgttca
     6121 acagcgcgtg tttggtccag tagtggaagt gctcggtcag acgataggtg gttccgacat
     6181 acgggaactt atcggcttta cccaatgctt cggcgtcgtc tttaaagata cgtgcagctg
     6241 ggttcgagat aacgtttgga tgcagcgggt tagttcccag cggcgtttca aacggctcgt
     6301 agtgttccgg gaacggacct tccgccatct tatcgagggc aaacagacgc cccatgcctt
     6361 cctgctgcat gataaacggc ccgacgccgc tacccggagg cgctgcgctg tagtccggaa
     6421 tatcccagcc ggtccactta gtgccgtccc atttcagcaa ctgacgcttc ggatcccacg
     6481 ggttaccctg cggatctgcg gaggcgcggt tatacagaat gcggcggtta agcggccatg
     6541 cccatgccca gcccagcgtg ttaccgaggc cagacggatc ggcgttatca cgacgcgcca
     6601 tctggttgcc ttccggcgtc cagctaccgg cgaaaatcca gcagccacag gaggttgtac
     6661 cgtcatcgcg caattgggcg aacgagctaa gttgttggcc tttcttgacg ataaccgcac
     6721 cggttgccgg atcggtaata tcggccagcg ccttaccgtt gctctccatc gctacttctt
     6781 ccgacttcgg ctcatgcgga atggcgtagt tccaggtcat gttcagcacc tgatccgggt
     6841 tcgcgccacc ctgttcggca tacatcttgc gcaagcgcag gaagataccg gagaggatct
     6901 caccgtcagt cagcgcaatc cccggggcgt ccgcaccttt ccagtgccac tgcaaccagc
     6961 gacctgagtt aacgattgaa ccgttctctt ctgcgaagca ggtcgatggc agacggaaca
     7021 cttcggtctg gatcttcgac gagtcaactt cgttcagctc accgtggttc tgccagaagt
     7081 tagaggtttc agtgttcagt gggtcgatgg tgacgaggaa cttcagtttc gacagacaac
     7141 cgatcacttt gtttttgttc gggaatgagg caacagggtt aaagccctgg cagatatagc
     7201 cattgacctt gccctctttc atcatctcga agtattgcag gacgtcgtag cctttatccc
     7261 acttcggcaa ccagtcaaag ccccagctat tttccgccgt cgctttatca ccaaagaagg
     7321 ccttcatcat agagacgaag aatttcgggt agttgcccca gtagtttacc tggccttcca
     7381 gcagcggttt tggcgtgttg gcggtaaggt aggtttgcag atcggtctgc ttctcgcttg
     7441 gcagcgtcat gtaacctggc agactctgcg acagtagccc caggtccgtc agcccctgaa
     7501 tattggagtg accgcgcagg gcgttaacgc cgccgccggc catccccata ttgccgagca
     7561 gcagctggat catcgccatc gtacgaatgt tttgcgcacc aacggagtgt tgcgtccagc
     7621 cgagggcata caggaacgag gcagttttat cgtgagcact ggtttctgcg atgtattcgc
     7681 agactttcag gaacgcgtct tttggcgtac cacagatgtt ttcaaccaca tctggcgtgt
     7741 agcgggaaac gtgctgtttc agcaagttcc acacgcagcg cggatgttgc agcgtggtat
     7801 cgcgtttggc gaagccgttt tcgtccagtt cataagtcca ggaggattta tcgtacttgc
     7861 gtttttccgc gtcgtagccg gtgaacaggc catcttcaaa gccgtaatcc tcacgcacga
     7921 tcaggctggc gttggtatag gcttcggtgt attcgtggtt gaatttttca ttgttcagca
     7981 ggtacagcaa tacgcctgac aggaaagcaa tgtcagtacc ggaacgaata ggggcatagt
     8041 agtcagctac cgccgccgta cgcgtaaagc gaggatcgat cacaatcagc ttcgcgccgt
     8101 tgtgaatttt ggcttccatc gcccagcgga acccgaccgg gtgagcttca gcggcgttac
     8161 cgcccatcac cacgacgagg ttggcgttct tgatgtcgac ccagtggttg gtcatcgcac
     8221 cgcgaccaaa tgttggagca agacttgcta ccgttggtcc gtgtcagaca cgcgcctggt
     8281 tgtcgaccgc gagcataccc agcgcgcggg agaatttttg cgttaaatag ccggtttcgt
     8341 tactcgacgc ggaagcacac agcatcccgg tggagagcca gcggttaaca gtcacgcctt
     8401 cggcgttttg cgcaatgtag ttagcatcgc ggtcttcttt catcagtttg gcgatgcgat
     8461 caaacgcctc ttcccaactg atttgctgcc atttatcaga acctggcgca cggtattccg
     8521 gaaacttcag acggctttcg gagtggatga aatccaccag gccagcgcct ttcggacaaa
     8581 gtgcaccgcg gttgaccggg tgatccggat cgccttcgat atggaagata gatgctttgg
     8641 cgttttttgc tccgtcaccg aggctgtaca tcaacagccc acagcctacg gaacaatagg
     8701 tgcaggtatt acgggtttcg cgggtgcgca gcagtttata ctgccgggtt tccgcgagtg
     8761 ctacgctggg tgcaaaaccc agtgccgctg ccgtggtgcc tgccataccg ccagcgcaga
     8821 tcttaaagaa ctgccttctg ctgacctgca tggattgctc cttgtttcga cattgtcacg
     8881 tcccatttac attcgcttgc tgcgtgtgca gggagtggga gttatttttc tttgcggaag
     8941 gggccgcaaa ggtccagaat tggctcaatt tccctccatc taggaagggt ttgtaacaga
     9001 ataccataat gttggtgtgt atgttcttat ctggttaaga gaaagtgaaa aaaacacagc
     9061 gaaaagaaat cgaaaatgtg acaaatatca caggtgttcg tcaaattgag ttatggcggc
     9121 gtgatgattt acaacaccca cggttagatg aggtcgcgga agaagttccc gttgcgctgg
     9181 tctacaacgg catttcgcat gtggtgatga tggcgtcgcc caaagacctt gagtactttg
     9241 cgctcggttt ttcgctttcc gaggggataa tagaaagtcc gcgcgatatc ttcggcatgg
     9301 atgtcgttcc ttcctgtaat ggtcttgaag tacaaattga gctttccagc cgccgcttta
     9361 tggggttgaa ggagcgccgc cgggcgctgg cgggacgtac gggctgtggc gtatgcggcg
     9421 tggagcaact taatgacatc ggaaaaccgg tgcagccgct accgttcacc cagacgtttg
     9481 atctcaacaa actggatgat gcattacgtc atctcaacga ttttcagcca gtggggcgac
     9541 tgactggttg tactcacgcc gctgcctgga tgttgccatc tggcgaactg gtcggcgggc
     9601 atgaagacgt gggtcgccat gtggcgctgg acaaactgtt aggccgccgg tcacaagaag
     9661 gggaaagctg gcagcaaggt gcggtactgg tttccagtcg tgccagttat gaaatggtgc
     9721 aaaagtcggc gatgtgcggc gtagagattt tgtttgcggt gtctgccgcg accacgcttg
     9781 ctgtagaagt ggctgagcgc tgtaatctga cactggtagg tttttgtaaa ccgggtcggg
     9841 caacggttta tacccatccg cagcgtttga gcaattaatt aaaataagtc ctgcgatata
     9901 tattaaattt caggacttat tcatttc
//
LOCUS       DAEEUE010000128         9905 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.128, whole genome shotgun sequence.
ACCESSION   DAEEUE010000128 DAEEUE010000000
VERSION     DAEEUE010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9905
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.128"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(160..900)
                     /gene="pflA"
                     /locus_tag="KLK84_003549"
     CDS             complement(160..900)
                     /gene="pflA"
                     /locus_tag="KLK84_003549"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HBE2010728.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(1092..3374)
                     /gene="pflB"
                     /locus_tag="KLK84_003550"
     CDS             complement(1092..3374)
                     /gene="pflB"
                     /locus_tag="KLK84_003550"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBE2010729.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3429..4286)
                     /gene="focA"
                     /locus_tag="KLK84_003551"
     CDS             complement(3429..4286)
                     /gene="focA"
                     /locus_tag="KLK84_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HBE2010730.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4692..6452)
                     /locus_tag="KLK84_003552"
     CDS             complement(4692..6452)
                     /locus_tag="KLK84_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HBE2010731.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEDFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6582..7208
                     /locus_tag="KLK84_003553"
                     /pseudo
     CDS             6582..7208
                     /locus_tag="KLK84_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            7407..8495
                     /gene="serC"
                     /locus_tag="KLK84_003554"
     CDS             7407..8495
                     /gene="serC"
                     /locus_tag="KLK84_003554"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HBE2010732.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8566..9849
                     /gene="aroA"
                     /locus_tag="KLK84_003555"
     CDS             8566..9849
                     /gene="aroA"
                     /locus_tag="KLK84_003555"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBE2010733.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         2520 a         2368 c         2482 g         2535 t
ORIGIN      
        1 ttcgatgggg aagaacttca cgcgagctat tatcagtcgt tattatctca ggtacgggat
       61 atggtagatg cactataaga tgtgttaaaa acgctgtagc agaatgaagc gcggaataaa
      121 aaagcggcaa ctcaataaag ttgccgcttt acgaggaaat tagaacatta ccttatgacc
      181 gtactgctca agaatgcctt tcacgcgttc catggtctct ttcttcggtg gtttaacacc
      241 atcgagtttg tattcttcac ccatcgccac ccatttgtgt ttgcccagtt cgtggtaggg
      301 gagaagttcg attttctcaa cgttacccat atcacgggta aattcaccga ggcgatgtgc
      361 cgaatcgtca tcgtcagacc agcctgggac aacaacgtag cggatccaca ccttcacatt
      421 tttgttcgcc agatatttag cgaactccag cgtgcggtgg ttggaaactc caaccagatt
      481 ttggtggatc tcgtcgttca tctgtttgag atcgagcatt accaggtcgg ttacttccag
      541 cagttcatca atcaccggat cgtaacgacg aacaaaaccg ttggtatcca gacaggtatg
      601 aatgccttct tttttacagg cgcggaacca gtcacgaaca aactcagctt gcaggattgc
      661 ttcaccgccg gatgcggtaa cgccgccgcc ggaagcgttc ataaagtggc gataggtcac
      721 cacttccttc atcaaatctt caacggtaac ttctttaccg ccatgcgtgt cccaggtgtc
      781 gcggttatga caatacaggc agcgcatcag gcagccctgg aaaaaggtga taaagcgaat
      841 acctgggccg tctacggttc cacaggattc aaaggagtga atgcgaccaa taactgacat
      901 tgcggtgttt ctccagatgt ggcccatctg aggccgtgtt ggtgcgcagc tcgaaggcta
      961 cgtcgagtct gttttggcag tcaccttaaa gtatagatag ctgacaaaaa aggctctcgt
     1021 gctaaaaaag gccccacttt cgtggagcct ttattgtacg ctttttactg tacgatttca
     1081 gtcaaatcta attacataga ttgagtgaag gtacgagtaa taacgtcctg ctgctgttct
     1141 ttagtcagcg agttgaaacg tactgcgtag ccagatacac ggatggtcag ctgcggatat
     1201 ttttccgggt tttccatcgc gtcgagcagc atttcacggt tcatcacgtt aacgttcagg
     1261 tgctgaccac cttcgatgga tgcttcgtgg tggaagtaac catccatcag accagccagg
     1321 ttggtcttac gaacttcgtc gtctttaccc agtgcgttcg gaacgataga gaaggtgtag
     1381 gagataccat ctttagcgta agcaaacggc agtttagcaa cggaagtcag agacgctaca
     1441 gcacctttct ggtcacgacc gtgcatcggg ttagcacccg gtccgaacgg cgcgccagca
     1501 cgacgaccgt ctggggtgtt accagttttc ttaccataca caacgttaga agtgatggtc
     1561 agaacagact gagtcgggat agcgtcacgg taggtgtgca gtttctgaat tttcttcatg
     1621 aaacgttcta ccaggtcaac agccaggtca tctacacgcg gatcattgtt accaaactgc
     1681 gggtattcgc cttcgatttc gaagtcgata gccagaccgt cttcgtcacg aatcggttta
     1741 actttcgcat atttgattgc agacagggag tcagcagcaa cggacagacc agcgatacca
     1801 cacgccatgg tgcggataac gtcacggtcg tgcagcgcca tcagagaggc ttcgtagctg
     1861 tacttgtcgt gcatgtagtg gatgatgttc agtgcagtga tgtactgttt agccagccag
     1921 tccatgaagt gatccatgcg ctccatcact tcatcgtagc tcaggagatc gcctttgatc
     1981 ggttcagact tcggaccaac ctgcattttc agtttttcgt caacgccgcc gttgattgcg
     2041 tacagcatgg ttttcgccag gtttgcacgc gcaccgaaga actgcatttg tttaccaacg
     2101 atcatcgggc ttacgcagca agcgatagcg tagtcatcgt tgttgaagtc cggacgcatc
     2161 aggtcatcgt tctcatactg cagagaagag gtgtcgatgg acactttagc ggcgaatttc
     2221 ttgaagttca gcggcagttt ttcagaccac agaatggtca tgttcggttc cggagacggc
     2281 cccatagtgt acagggtgtt caggaaacgg aagctgtttt tggtaaccag ggtacgacca
     2341 tcgaggccca taccaccgat agattcggtt gcccagattg ggtcgccaga gaacagttca
     2401 tcgtattccg gagtacgcag gaagcgaacc atacgcagtt tcatgaccag gtggtcaacc
     2461 atttcctgcg cttcttgttc ggtgatcttg ccagctttca ggtcacgttc gatgtacaca
     2521 tccaggaagg tggaggtacg accgaaggac attgcagcac cgttctgaga cttaacagca
     2581 gccaggtagc cgaagtaagt ccactggata gcttcctgag cgttggtagc cggaccagag
     2641 atgtcgtagc cgtatttcgc agccatttct ttcatctgac ccagagcgcg gtgctgttca
     2701 gcgatttctt cgcgcagacg gatagtctgt tccaggttta cgccgttttc cagatcagcc
     2761 tgtagagagg tgaactgagc gtatttgtct ttcatcaggt agtcgatacc gtacagcgca
     2821 acgcgacggt agtcaccgat gatacggcca cggccataag catctggcag accggtcaga
     2881 acaccggatt tacggcaacg caggatgtcc ggagtgtaaa cgtcgaacac gccctggttg
     2941 tgagttttac ggtattcagt gaagattttt ttgatcatcg ggtccagttc gcggttgtac
     3001 gctttgcagg aaccttcgat cattttgata ccaccgaacg ggataagagc acgtttcagc
     3061 ggagcttcag tctgcagacc aacaactttt tccaacgctt tattgatgta gccagcgtcg
     3121 tgagaggtga tggtggaagc aacagcggtg tcaaaatcaa ctggcgcgtg agtgcggttt
     3181 tccagtttaa caccttccat tactttgtcc cacagggtgg tggtcgcttc agtagcgcca
     3241 gccaggaagg actcgtcacc ctcgtacggc gtgtagtttt tctgaatgaa gtcacggacg
     3301 tttacttcat tctgccagtc acctttggta aaaccttccc aggctgtggc taacttttca
     3361 ttaagctcgg acatgtaaca cctaccttct taagtggatt ttttatttac tgcgtacttc
     3421 gacaaccatt aatggtgatc gttttcacgc aggtaaatga cccagtatgt caacccaacc
     3481 aacaaaccac cgccgataat attaccgatc gtaaccggga tcaggttatc agtgatgaag
     3541 ttcatcacgg tcaggtgaga aaaattttcc ggtgcagaac cgacagcggt ccagaattcc
     3601 ggagatgcga agtcgcggat tacaataccc atcgggatca taaacatgtt tgcgatactg
     3661 tgctcaaaac cgctggcaac aaacatcgcg accggcagca ccataatgaa cgctttgtcc
     3721 atcaggctgc ggccagaata actcatccat actgccagac ataccatcag gtttgccagg
     3781 ataccaagac agacggcctc aataaaagta tggtgcactt tgtggtcggc ggtttgtagg
     3841 acgtttagtc cccattgacc atttgcggtc atatactcgc cggaaagcca cattaaaagt
     3901 acaaacagca gtgcgccgac caggttgcca aaatagacat ttagccagtt tttagccaac
     3961 tgacaccagg tgatgcgccc actcgcctta gcaacaacaa tcaacactgt ggaagtaaag
     4021 agatcggctc cgcagacaac acaaagaatc agccccagag agaagcaaat gccgccaacc
     4081 agttttgcca tgccgaaggg cattgtgcct gtgccagtgg ttgctgtgat atagaagacg
     4141 aatgcgattg agatgaaaac accggcggta atcgccagat agaaagtctt aagcggatgt
     4201 ttcgttgctt tatagacacc cgcctcttcg gccactttgg ccattgcagc aggaagtaaa
     4261 agatcaaaag ggttgtcagc tttcacacta actctctctt tattaagtcg gcgacgagat
     4321 actaacaaag cattatagat gagaaattga tatagatcat atctcgcctg gcttataggc
     4381 ccgtaactcg catggttttt atgcaaatac ggagtaacta tttgattatc caaataaaaa
     4441 taaattttaa aaattaacaa atgagttgaa ttttttccgc atcctccgct aaaacagtta
     4501 attaaaaggg agcatcaggc gaataaagta acaatatcga tcgtatttat taaatacaaa
     4561 ttaccgatat ttaaccttat aattacaatt attttattaa tgcaaatata tgtaaagcgg
     4621 ggcattaaaa aaacgccccg taatataact cagactaatc attaagccta cattgcgtag
     4681 gctatttgat tttattttgc ccagaatgct gctttggcgc gctgcagctt ttcgtaggcc
     4741 ttcaacaacg actgatgtgc agcaaacgcg tgcagatcgc tatctaccgg ttgcaggccg
     4801 taaaacgccg cttcgccgct cattgccgca ctggcggctt ctacggcatc tgcgccgtac
     4861 atgcgaacaa acgcattcag atattgcagc ggctggcgat cttcttcctg tgccagtaat
     4921 aacaacgttt gcaggcagcg ataatagttg gcgcgttccg gactaaatac tgatgagtta
     4981 aactccatcg tccattcggt ccagaccaga gcctgttcca gatcgccacc agccagcgcc
     5041 agcatggctt ttaattcacc gatacgcagg gtgtaccaac cgttatccga cccggtcgcc
     5101 agacccaaca gctcacgcac gcgggtaaag tcatcaaaat cttcttcatc cagttgctcg
     5161 atgaggttca ggtaatcttc tttttcccac tcgctgcctg gtagcgaaag aatcgtttca
     5221 cgtaaatggc tgcccatact gttattcgcg agccacagat cttcagccgg ataaatatcg
     5281 gacatgccag gcacgataat acggcaagca taaacgccca gatgctcgta atcggcaata
     5341 taaacttctt tatcttcttt gttgaagata gccatcagcg tagcaaactc ttcttccgtg
     5401 gtgccggaga aattccagtc cacaaacgga taatcggcat cctgcttgaa caggtcccag
     5461 gagattaaac cgctggaatc gataaagtgc gtttcgaggt tggtatgttc agcgacttct
     5521 tcatcatcga aggttggcgg agtaaacaca tccaaatctt tcaggccacg accttgcagc
     5581 agctcggtca cggtacgttc cagtgctacg ccaaaatcag gatgcgcacc gaaagaggca
     5641 aagcaggtgc cgtttgcagg attaaacagt accacgcaaa tcaccggata ctggccgcca
     5701 agggaaccat catatgcgaa gatcgggaaa ccttctgttt ccagtgtttc gatcgcttca
     5761 accaccgctg ggtaacgcgc cagcacgtct gccgggattt ccggcaagct gatgctttca
     5821 gcaataatgc ggtttttcac gtagcgttcg aaaacttcgg acaacccctg aacgcgtgct
     5881 tcgttgcggg tattgccagc ggacataccg ttagaaacgt acaggttacc aatgatattc
     5941 atcggaatat aaacggtctg attgtcggac tggcgcgtaa acggcagacc gcaaatacca
     6001 cgatcttcgt taccggattg taggtcaatc agcatactgc cggtcaattc attctccgga
     6061 tcgtaaaatg cgcgcagacg gtcatcgagc agcccttctg gcacatcgtc attttcgctc
     6121 agtgggaacc atttttcgtt gggataatgc acaaatggac cgttggcgat ggtttcgccc
     6181 agccagaaat cagcaaaaaa gtagttggtt gagagacgct cgaaatattc accgagtgca
     6241 gaagccagcg ccgctttctt ggttgcgcct ttaccgttgg taaaacacag tgcgcactct
     6301 ttgtcgcgaa tatgtacaga ccagacgtta ggcacgggat tcagccagga ggcctcttca
     6361 atctgaaagc cgaggtctga aagtttttgc tggaagcgag cgatggaatc ttccagagcg
     6421 gcatctttgc cggggataaa tgtttgcgtc atgaaaatca ctttagtcgt acggaaagcg
     6481 cgcaataata cgggttttat ctcaaaggcg ctatcaccgc cgccatgccg atgaactgtt
     6541 gactatgctt ttagcggata aaccacgtta agagcataaa aatgaaggcg ttcgatctcc
     6601 accgtatggc atttgataaa gtgccttttg atttccttgg cgaagttgca ctacgtagtc
     6661 tttatacctt tgtactggtt ttttgttcct caaaatgacc ggaagacgcg gtgtgcgaca
     6721 gatgtcgctg tttgaagtgt taatcattct cacgctgggt tcagcggcgg gagatgtggc
     6781 gttttatgat gatgtgccga tggtgccggt acttatcgtc tttatcactc tggcgttgtt
     6841 ataccgcctg gtaatatgat ggcgagctgg cctggtcgaa actcaataac tccaacatga
     6901 cggaatttga gttctttatg gagctacgat tgcgtggcgt ggagcagctg gggcaggtac
     6961 gtctggcgat tctcgaaacc aacgggcaaa tcagtgtcta tttctttgaa gatgacaagg
     7021 tgaaaccggg tttacttatt ttacccagtg attgtacgca gcgttacaaa gtggtgccgg
     7081 agtcggcgga ctatgcctgc attcgttgta gtgaaatcat tcatatgaac gcgggggaaa
     7141 aacaattatg tccgcgctgt gcaaatccag aatggacgaa ggcaagtcgg gcaaaacggg
     7201 tgacctgaca gtaaaaacat cggctttttg ctaataatcc gagagattct tttgtgtgat
     7261 gcaagccaca tttttgccct caacggtttt actcattgcg atgtgtgtca ctgaatgata
     7321 aaaccgatag ccacaggaat aatgtattac ctgtggtcgc aatcgattga ccgcgggtta
     7381 atagcaacgc aacgtggtga ggggaaatgg ctcaaatctt caattttagt tctggtccgg
     7441 caatgctacc ggcagaggtg cttaaacagg ctcaacagga actgcgcgac tggaacggtc
     7501 ttggtacgtc ggtgatggaa gtgagtcacc gtggcaaaga gttcattcag gttgcagagg
     7561 aagccgagaa ggattttcgc gatcttctta atgtcccctc caactacaag gtattattct
     7621 gccatggcgg tggtcgcggt cagtttgctg cggtaccgct gaatattctc ggtgataaaa
     7681 ccaccgcaga ttatgttgat gccggttact gggcggcaag tgccattaaa gaagcgaaaa
     7741 aatactgcac gcctaatgtc tttgacgcca aagtgactgt tgatggtctg cgcgcggtta
     7801 agccaatgcg tgaatggcaa ctctctgata atgcggctta tatgcattac tgcccgaatg
     7861 aaaccatcga cggtatcgcc atcgacgaga cgccagactt cggcaaagat gtggtggtcg
     7921 ccgccgactt ctcttcaacc attctttccc gtccgattga cgtcagccgt tatggtgtaa
     7981 tttacgctgg cgcgcagaaa aatatcggcc cggctggcct gacaatcgtc atcgttcgtg
     8041 aagatttgct gggcaaagcg aatatcgcgt gtccgtcgat tctggattat tccatcctca
     8101 acgataacgg ctccatgttt aacacgccgc cgacatttgc ctggtatcta tctggtctgg
     8161 tctttaaatg gctgaaagcg aacggcggtg tagctgaaat ggataaaatc aatcagcaaa
     8221 aagcagaact gctatatggg gtgattgata acagcgattt ctaccgcaat gacgtggcga
     8281 aagctaaccg ttcgcggatg aacgtgccgt tccagttggc ggacagtgcg cttgacaaat
     8341 tgttccttga agagtctttt gctgcaggcc ttcatgcact gaaaggacac cgtgtggtcg
     8401 gcggaatgcg cgcgtctatt tataatgcca tgccgctgga aggcgttaaa gcgctgacag
     8461 acttcatggt tgagttcgaa cgccgtcacg gttaatgccg aaattttgct taatccccac
     8521 agccagcctg tggggttttt atttctgttg tagagagttg agttcatgga atccctgacg
     8581 ttacaaccca tcgctcgtgt cgatggcact attaatctgc ccggttccaa gagcgtttct
     8641 aaccgcgctt tattgcttgc ggcattagca cacggcaaaa cagtattaac caatctgctg
     8701 gatagcgacg acgtgcgcca tatgctgaat gcattaacag cgttagggtt aagctatacg
     8761 ctttcagccg atcgtacgcg ttgcgaaatt atcggtaacg gcggtccatt acacgcagaa
     8821 ggtgccctgg agttgttcct cggtaacgcc ggaacggcaa tgcgtccgct ggcggcagct
     8881 ctttgtctgg atagcaatga tattgtgctg accggtgagc cgcgtatgaa agagcgcccg
     8941 attggtcatc tggtggatgc gctgcgcctg ggcggggcga agatcactta cctggaacaa
     9001 gaaaattatc cgccgttgcg tttacagggc ggctttactg gcggcaacgt tgacgttgat
     9061 ggctccgttt ccagccaatt cctcaccgca ctgttaatga ctgcgcctct tgcgccggaa
     9121 gatacggtga ttcgtattaa aggcgatctg gtttctaaac cttatatcga catcacactc
     9181 aatctgatga agacgtttgg tgttgaaatt gaaaatcagc actatcaaca atttgtcgta
     9241 aaaggcgggc agtcttatca gtctccgggt acttatttgg tcgaaggcga tgcatcttcg
     9301 gcttcttact tcctggcagc agcagcaatc aaaggcggca ctgtaaaagt gaccggtatt
     9361 ggacgtaaca gtatgcaggg tgatattcgc tttgctgatg tgctggaaaa aatgggcgcg
     9421 accatttgct ggggcgatga ttatatttcc tgcacgcgtg gtgaactgaa cgctattgat
     9481 atggatatga accatattcc tgatgcggcg atgaccattg ccacggcggc gttatttgca
     9541 aaaggcacca ccacgctgcg caatatctat aactggcgtg ttaaagagac cgatcgcctg
     9601 tttgcgatgg caacagaact gcgtaaagtc ggcgcggaag tggaagaggg gcacgattac
     9661 attcgtatca ctcctccgga aaaactgaac tttgccgaga tcgcgacata caatgatcac
     9721 cggatggcga tgtgtttctc gctggtggcg ttgtcagata caccagtgac gattcttgat
     9781 cccaaatgca cggccaaaac atttccggat tatttcgagc agctggcgcg gattagccag
     9841 gcagcctgaa tgaacaacgg gcaataaata gccaaatctt tctttatcaa aacgtcggca
     9901 cattg
//
LOCUS       DAEEUE010000129         9783 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.129, whole genome shotgun sequence.
ACCESSION   DAEEUE010000129 DAEEUE010000000
VERSION     DAEEUE010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9783
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.129"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..143
                     /locus_tag="KLK84_003556"
     CDS             <1..143
                     /locus_tag="KLK84_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBE2010734.1"
                     /translation="AEVELATLTWVDWYNNRRLLERLGHIPPAEAEKAYYASIGNDDL
                     SA"
     gene            complement(194..514)
                     /gene="psiF"
                     /locus_tag="KLK84_003557"
     CDS             complement(194..514)
                     /gene="psiF"
                     /locus_tag="KLK84_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HBE2010735.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(633..2048)
                     /gene="phoA"
                     /locus_tag="KLK84_003558"
     CDS             complement(633..2048)
                     /gene="phoA"
                     /locus_tag="KLK84_003558"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HBE2010736.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2149..2409)
                     /gene="iraP"
                     /locus_tag="KLK84_003559"
     CDS             complement(2149..2409)
                     /gene="iraP"
                     /locus_tag="KLK84_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HBE2010737.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2591..2794)
                     /locus_tag="KLK84_003560"
     CDS             complement(2591..2794)
                     /locus_tag="KLK84_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010738.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2872..3966
                     /gene="ddlA"
                     /locus_tag="KLK84_003561"
     CDS             2872..3966
                     /gene="ddlA"
                     /locus_tag="KLK84_003561"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBE2010739.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3990..4202)
                     /locus_tag="KLK84_003562"
     CDS             complement(3990..4202)
                     /locus_tag="KLK84_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HBE2010740.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4462..4770
                     /locus_tag="KLK84_003563"
     CDS             4462..4770
                     /locus_tag="KLK84_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HBE2010741.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4829..5923)
                     /gene="yaiW"
                     /locus_tag="KLK84_003564"
     CDS             complement(4829..5923)
                     /gene="yaiW"
                     /locus_tag="KLK84_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HBE2010742.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPRKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5936..7156)
                     /gene="sbmA"
                     /locus_tag="KLK84_003565"
     CDS             complement(5936..7156)
                     /gene="sbmA"
                     /locus_tag="KLK84_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HBE2010743.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVAHITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7508..8665
                     /gene="ampH"
                     /locus_tag="KLK84_003566"
     CDS             7508..8665
                     /gene="ampH"
                     /locus_tag="KLK84_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HBE2010744.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8666..9169)
                     /locus_tag="KLK84_003567"
     CDS             complement(8666..9169)
                     /locus_tag="KLK84_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE2010745.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(9257..>9783)
                     /locus_tag="KLK84_003568"
     CDS             complement(9257..>9783)
                     /locus_tag="KLK84_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBE2010746.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         2437 a         2493 c         2333 g         2520 t
ORIGIN      
        1 gtgcagaagt ggaactggcc acactaacgt gggtggactg gtataacaat cgacgattgc
       61 tggaaaggct gggccacatc cctccggcag aagcagaaaa agcttattat gcttccatcg
      121 gaaacgatga tctgtcagcc tgagttcaca gataaaacac tctccaggaa acccggggcg
      181 gttcaatcag gtatcaggcg gctttcttga ggcaggcact cataaactta ttacgatcat
      241 cacctttcag agcttgttgc gttgcttgat tattacattc acgcatcttt tgctgctgtg
      301 gcgtcaaact tttttcacca ggcgcagact tgctgttctt caggcaatca ctcatgtagg
      361 tcttacgagc atcccctttc aacgcctgcg ccgtcgcctg ctgattacag gaggtcatac
      421 gctgttgttg tggggttaag gttctctcgg cagcgccgac ggtggttaaa aaaaccagac
      481 caaaaagcaa ggtaaccagt aatgttattt tcatagcacc atccctcttc atgttttaac
      541 catgagcgtt tgcgcccgtg atctgccatt aagtctggtt gctaacagca aaaaaaccac
      601 ccggcagcga aaattcactg ccgggcgcag ttttatttca gccccagagc ggctttcatg
      661 gtgtagaaga gatcggtctg gtcggtcagt ccaacaacat tggcggcatg cgggccatac
      721 gccgcaatac gcaactgact gccggtatgt tcttgtgaat cctcttcgga attcccgtaa
      781 ctcatcacca tcactgcgcc atctttagta tttagcgcct gagtgaggcc cggcgctttg
      841 gtatccggcg caacaacctg gctggcgtgg gcgtgatcag cggtgactat gaccagcgtg
      901 ttaccctcct ttttagcgaa ttccagcgcc cgttgtacgg cttcatcgag atcgaccgtc
      961 tcgccaattt gcccacaagg gttcgcagcg tgatcctgtt tatcgattga cgcaccttca
     1021 acttgcagga aaaatccttt ctcattttta ctcaacaatt caatggcttt gtcggtcatc
     1081 tgtgccaggg ttggtacact gtcattacgt tgcggattag gcgtataggt gactgcgggc
     1141 ttatcgatat tgccgtggta cgttgctttc ggccctagcc agcgcactgg catattgccg
     1201 tcagcaaaca ggccaagcag gggtttttgc tgattcgctt ccgtcaccga attcagtgag
     1261 gcagcatcgt tcaccaactg ataaccacgc gcctgtgcct gttcacgcag cgtttttccc
     1321 tgccattcac cagcggttgc cgtttcagca aaggtttttg cgccgccgcc aagcgtaacg
     1381 tcggcacgag cgttaagcag ctgttcggta atcgatcctt ttccgccttt ttccagagcg
     1441 ttacccggac atttttcact ggtcgcgctc ggaccgtagc atttgcgcga agtcacatgt
     1501 gccaccagcg cagcgggcgt ggcatcctgc aactctgcgg tagaaacgtt accggtcgcc
     1561 agacctgcgg cttttgccat ttccagaatc gttgggtgat ctttttcgtg aatatcgacg
     1621 cccagcgcgc cgttataggt tttgacaccg gttgaccagg cggttgctga tgcagccgag
     1681 tcggtgacgt agtccggttt gccagttttt ttattcagcg catagtgagt gtattgcccg
     1741 gtaagcggta aggcatcgat acctttaaaa aagccgcccg caccttcggc ataattacgt
     1801 gcggcagtaa tttccgaatc ccccatccca tcgccaatca gcaaaataat attttttgca
     1861 ggtttatcgc taagagaatc acgcagagcg gcggtctgat cacccgttaa gcggcgagca
     1921 ccgccgggtg cagtaatatc gccctgagca gcccggtttt ccagaacagg catttctggt
     1981 gtccgggctt ttgtcacagg ggtaaacagt aacggtaaga gtgccagtgc aatagtgctt
     2041 tgtttcactt tattttctcc atgtacaaat acattaaaaa ataaaaacaa agcgactata
     2101 agtctcggcc gtgacaactt tatgacagct gttgaaaaga ttaacttttt actgacaagg
     2161 atgcttcaat aacttcttta cgtaatcgcg cagcagctct gtatcgtcgt caggaatgct
     2221 ggcatcgggc tttacctcgt acagcgcccc ctctacctga tcaatcaacc gctgttggtc
     2281 attttgcgcc atattgcgaa gcattgcagt gacgataatc tccaaagctt ctacctgcgc
     2341 acacagttct ttcgactctt cttctttttg ggcaagctta aataacaact cagcaatgag
     2401 atttttcatg tctgtatttc cttatccaaa gtatggagaa gttaacatta tgtttcacaa
     2461 cttcatagtg tctggaagta ttatttttat gacaaaggaa atactttgcg caaatatttt
     2521 ttgtttgatt accagcgaaa cgtttctctt atttaaataa ttacccacac aaaatatagg
     2581 cttttaatta tcaaccttta ttataaataa aagctatgaa atatattctc ttcgttaact
     2641 tataattaag agaaaaaaac tcaccgcatt gccatttaag ccaggaataa attagcttcc
     2701 cgttcatcat ttttgtgatc ttgcgcacat ctttgagata acgcactttc ttcaccagta
     2761 cgttgcacgc tttgtcagca gaagattgca acaatctttc agaaggccgc gaatcccccg
     2821 ttttgtcgtg gtaatgtatg cactttgtgt tagacgggat aggtttttaa gatggaaaaa
     2881 ctgcgggtag gaatcgtttt tggtggtaaa tcagcggaac atgaagtgtc tctgcaatcg
     2941 gcaaaaaaca ttgtcgatgc cattgataaa agtcgcttcg acgttgtgct gctgggcatt
     3001 gataaacaag ggcaatggca cgtcagcgat gccagcaatt atctgctaaa tgcagacgat
     3061 cctgcccata ttgcgttgcg cccttcggcg accagccttg cgcaggtgcc aggtaaacat
     3121 gagcatcagc ttatcgacgc gcaaaacggt cagccgttgc cgacggtgga tgtcattttc
     3181 ccgattgtcc acggtacgct gggcgaagat ggttccttgc agggaatgct gcgggtcgcc
     3241 aatttaccgt ttgtaggttc tgatgttctg gcttcagcag cctgtatgga taaagatgtc
     3301 accaaacgtc tgctacgcga tgccgggctg aacattgcgc catttattac cctgacgcgc
     3361 gctaatcgtc acaacatcag ttttgccgaa gtggagtcta aactggggtt accactgttt
     3421 gtaaaaccgg ctaatcaggg ctcttctgtt ggtgtcagca aagtaaccag tgaagaacag
     3481 tacgcaattg ccgtcgatct ggcgttcgag ttcgatcata aagtgatcgt tgagcaaggg
     3541 atcaaaggtc gtgagatcga atgcgcagtt ctgggcaacg acaatccgca agccagcacc
     3601 tgtggcgaga tcgtactcac cagcgatttc tatgcctacg acaccaagta cattgacgaa
     3661 gatggcgcga aagtggtagt tccggcagcc attgcaccag agatcaacga taagatccgg
     3721 gcgattgccg ttcaggctta tcagacgttg ggatgcgcag gcatggcgcg tgtagacgtg
     3781 tttttaaccc cagagaacga agtggtgatc aacgagatca acacactgcc tggcttcacc
     3841 aacatcagta tgtatccgaa gctgtggcaa gccagcggtc tgggttacac cgatctgatc
     3901 acacgtttga ttgagctggc gctggagcgt cacgctgcgg ataacgcatt gaaaaccaca
     3961 atgtaatatt ttaacgcccg ataatattct tattgggcgt tttcagtttc ttcatcgcga
     4021 cgacgaataa tcaaccccgc cagccagaaa ctaatcaccc acgtcaccag accgacggca
     4081 taggtctgcc aaccttttgc ttcaaatccc agtaccccca ccacgccatt aagaatgaat
     4141 ataaggccaa tggcgaacgc atagtagtgc cagtcacggc ggatttttac aggcagattc
     4201 atggcagctc cgttaacgaa aaacaacatc cttgcatatt ctgtaacaac cgtctgtggt
     4261 gtagacaaca ttttcgtaat tattaataaa tttaacttaa ggttgccatt ttgtgactca
     4321 caacgaagat tcacgcttcg ggaatattcc caacatcaat ttaccatgat tctgatattg
     4381 acaatctaaa tgacctgtca gacttgccat aagccgctgg tatcacacca gaagagaagc
     4441 agatgacttc cggaggtttt tatggctgat ttcaccctgt caaaatcgct gtttagcgga
     4501 aaatatcgca atgcctcttc aacgcctggc aacattgcct atgcgttgtt tgtgctgttt
     4561 tgcttttggg ctggggcgca attgctgaac ctgttagtgc atgcgcccgg cgtctatgag
     4621 cgtttaatgc aggtccagga aacaggtcgc ccacgggtgg aaattggttt aggtgtcggc
     4681 accattttcg ggctgatccc gtttttagca ggctgcctca tttttgcagt ggtggcgcta
     4741 tggctgcact ggcgacatcg ccgccagtaa aatttacgtt gccagatgcg gcctgaaacg
     4801 ccttatctgg cctgacattc ctgtttgatc actgtttcat acaacgcgcc cgccgttcgt
     4861 ctacgcgctt cgcaaaccag gccgtagtca ggttgcgggt gattttcggg ctttccagtt
     4921 gaatgccagg caacatttcg cggggtagtg atttacccgt tttcgcttcg gcaagttgat
     4981 aaactttctt gtacagcgcc gtctcctcaa aagagaagct gtcacctttc tctaactgac
     5041 ggcgaatttc gctgtcgctc atccccagtt ttcccgccag tttgcgcgtt gccagttcgg
     5101 ttttcctggg ttctttactg tcgtaacgaa ttaaatcacc gtccagtgcc agcttcacgc
     5161 cgctggcctt actgactgcg ttctgaaacg cggcattacg gctggcgtac cagccagcgt
     5221 taaaatcagc aaagcggtat atcggtgcgc tatagctggc gggataattc agtaaatggt
     5281 aagtaccaaa ccacaacccg ccgcggcgac tgaagacttc ctgacgaact gtaccgtcca
     5341 ttttccacgg ataccctttg gtatgctgtt cggcaaaagc aatgctgacc tgcatcggcc
     5401 caccggtgcg caccggatta agcgaaccaa acagcgtctg ccccattggt accatgctga
     5461 tcagatcgtc aaaaatcgcg ctcaattgct tttctgtacg caccgaatct aaccgttcgc
     5521 tataactttt gccgtttggc gatttgattt ttagcgcggt atgaaccaga aaagcaggaa
     5581 tgtgcatccg ttcagcacga cggtcaatct cttgccaggc gatcttgctt aagcccggaa
     5641 cggccggatc tgcctgataa tttgactcct gctgcgccac cgccagcacc gagtagacgt
     5701 tttccagcgt cggtgccagc ccctgacttt caaaggtggt ggcgagatct ttcgcccagg
     5761 catcgcggtc tttcacgctc gcaggcatct tctgccgcac gacgctcgct acatctatcg
     5821 ctttttcgcc ctttttcaac ggttgcggag cctgactact acaccccgct aaaaccagaa
     5881 ccgccaacaa tgacagcgaa gatagatgat tgacgcgcga cattacttct cctttttagc
     5941 tcaaggtatg ggttacttcc tgaattttgt caccatccag ctcatgttca aagctgcgca
     6001 gacgtttgta gatagacatc aactcaacca gtgtggtcca tgagttaatc aggtactgga
     6061 aagcaccgcg aacctgacca aaaacgttgg taatctgcgt catcaggccg agcgtaatcg
     6121 taccggcaac aattgacgga aacagcaaga acaaaccgaa aacgttatcg acctgcaaat
     6181 agagaatgcg ggcgatgttg aaatacatat agtgaaaata gaggcgaaaa tagtttttcc
     6241 gtacggcgct aaacagctcg cgtaccgtag gcggcgtcgc gcgcgtggca tcgtcttcac
     6301 cataaaccag ctctttacgg taggcggcct ctactcgctg gtttttaaac tccagccccg
     6361 gcagtttgat ccctaccact gccagcaatc cggtccccat cagcgaccag acgatagcgg
     6421 caatcaccag accatacgga atgtgcccga taatcggcag ctccggcaca tgcgcggaga
     6481 gcgttaccag caccggcagg aaggcgatca acgtcattat ggcgttgata aaactgacgc
     6541 ccatattctc cagcgttgaa gcaaaacgca tggtgtcttc ctgcacacgc tgtgcggcac
     6601 cttcgatatg acgcagttgt tgccagttcg ccatgtaata ttcgttcatc gccgtacgcc
     6661 agcggaacac gtagtgactg acaaagaagt tgttcagtac actgatcacc actgcgatca
     6721 gcgcaatccc cagaaagacg cccacttcgc ggtaaaattg ttcgatagtg actttatgcg
     6781 gcgaacttag cgcggtttga atcagatcat agaacggcgc ataccaggcg ttgacggcga
     6841 ccccgacttc caccaaaaac caagtgacga agataatcag cgcagtaccg agtattgacc
     6901 agtattgcca acggtgcggg ctgtagataa accagaaaaa tgcaaaaagt cctacgcaaa
     6961 caatgtagta agcgtaaaaa atcaggaaat ccaacgacca gaaacgcgcg gcgctaatcg
     7021 ggatctgccc ggaagcgccg gtgatatgtg ccacccagtc acccccaccg gcttgccaga
     7081 agataacggc aatcaatgcc caaacaaagg ccgagagaaa aaacgttccc ggctttggga
     7141 aaaaagactt aaacatatgc actcctgcta acttcttatc gttttaacaa ctgctgtgta
     7201 ttaaccgcat gaccgctaca aagcgtcagc caaatgaaac cgcccattgt gacccatagc
     7261 aaggcgctta gttcgccagc ctgaccataa gaattgtttc gacggatttc acaaaccaaa
     7321 gattgtttac ataagcggat taacacgcgc aggatcacaa atgcaggcat tggtgagcag
     7381 gaaaaagatc gtgtttacgc gccgttacgt ttttaacact attattgcac taattcgccc
     7441 tttgcaatcc atcaatgagt agtataaata cgctcagtta ccttcattca atctatggac
     7501 accaccgttg aaacgtagtc tgcttttttc tgccgtgctg tgtgcggcgt cattgacctc
     7561 cgtccatgcg gcgcagccga tcactgaacc ggagtttgcc tctgatattg tcgatcgtta
     7621 tgccgatcat attttttacg gcagcggtgc cacggggatg gcgctggttg ttatcgacgg
     7681 taaccagcgc gtctttcgta gttatggcga aacgcgacct ggtaataacg ttcgcccgca
     7741 gctggattcg gtcgtgcgta ttgcttccct caccaaacta atgaccagtg aaatgctggt
     7801 gaaattgctc gaccagggga ccgtaaagtt aaacgatccg ctaagtaaat atgctccgcc
     7861 aggcgcacgc gtgccgacct acaatgggac gcccattacc ctagtgaatc tggcaaccca
     7921 taccagcgcc ctgccccgtg aacagcccgg tggcgcggca catcgtccgg tgtttgtctg
     7981 gccaacgcgc gagcaacgct ggaaatacct ttctacggcg aagctgaaag ccgcgccagg
     8041 cagtcaggcg gcgtactcta accttgcgtt cgacctgctg gcagatgcac tggcgaatgc
     8101 gtccggcaag ccttataccc agttgtttga agagcagatt acccatccat tggggatgaa
     8161 agacaccacc tacaccccct caccggatca gtgccgccgt ttaatggttg ctgagcgcgg
     8221 tgccagtccg tgcaataaca cgctggcggc aattggcagc ggcggcgtct attccacgcc
     8281 tggcgatatg atgcgctgga tgcagcagta tttgtcatct gatttttatc agcgcagtaa
     8341 ccaggcagat cgcatgcaga cgttgattta tcagcgcgcg cagtttacga aagtgattgg
     8401 catggatgtc cccggcaaag cggatgcgct tggtttaggc tgggtatata tggccccgaa
     8461 agagggtcgt ccgaggatta ttcagaagac aggcggtggt ggcggtttca ttacctatat
     8521 ggcgatgatc ccgcagaaga atattggcgc gtttgtggtg gtcactcgtt cgccgttgac
     8581 gcgctttaaa aatatgagcg atggcatcaa tgatctggtg accgagctaa gcgggaataa
     8641 accgttggtt atccccgcat cctgattaat actctgaagg caaacggttg atagcgacca
     8701 gtttgccttt attcatttcg atatagccgc ctttacgcaa agcagcgaga acttctgcga
     8761 cgacagaacg cgatatgcgt gtacgttgat ggatataatt catcacacca atacgtgatc
     8821 gcaattcttc attccagtca atcattgata ataatgttgc gcgggtttgt ttggctggta
     8881 gatgatatcc cgtacaattt ccttctgata ttaatttgta tggtatatcg tttttcatta
     8941 aaccatcagc cagccccata atataaggag cctgggtaat accgataagt acgttttctt
     9001 ctctacgcag agagataact ccttctaaaa taacgaaagt atcttcgtta ttgacatcac
     9061 tggaaaatat aacttgcttt tcattattaa actcgaagcg cgtaccgtat ctggacaaac
     9121 atttatcgag cttaccaaat tcctgaagag gtttaactac agataacatt tgcgcgtcct
     9181 ttgcagtaat gcccgtcaaa tccttgacgg gcattattta gattaaatta ccagtatttc
     9241 ttcggagtga agaatattac caagtatatt taacacccac gttcgcggac cagtcttgat
     9301 ctacgtcacc accaccgagg tagttagcat cggtataggc gctgaagttc ttggtgaagc
     9361 tgaactgagt acccagacca acacgtaccg cagacccttc agtaccgtta tcgatggaat
     9421 caccgttcac atcgttatcg ttgttagagt cgtcgtagac gtaagccagt ttgaagtacg
     9481 gagtcagagc ctgatcttcg ctgtaggtga aggtataacc tgcatctaca cccagttcat
     9541 aacgcatgct gtcgtaaggc tgaccgtcaa ctttcatgtc gttgctcagc tggtagtcat
     9601 caccagactg gaacagacca gaaatgctgc cgtaaggagt cacgtaacca gcatcaccca
     9661 gtttgaagtc gtaaccggtt ttcaaaccga agccccaagc gtcggagttg gtgctaccgt
     9721 caacgtaagt accgttgctc atggttgcag acaggtcgtt gttgaagtga gagtagctca
     9781 agc
//
LOCUS       DAEEUE010000130         9644 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.130, whole genome shotgun sequence.
ACCESSION   DAEEUE010000130 DAEEUE010000000
VERSION     DAEEUE010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9644
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.130"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(384..1361)
                     /gene="epmA"
                     /locus_tag="KLK84_003569"
     CDS             complement(384..1361)
                     /gene="epmA"
                     /locus_tag="KLK84_003569"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HBE2010747.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1686..3494
                     /gene="frdA"
                     /locus_tag="KLK84_003570"
     CDS             1686..3494
                     /gene="frdA"
                     /locus_tag="KLK84_003570"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HBE2010748.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTIPTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3487..4221
                     /gene="frdB"
                     /locus_tag="KLK84_003571"
     CDS             3487..4221
                     /gene="frdB"
                     /locus_tag="KLK84_003571"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HBE2010749.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4232..4627
                     /gene="frdC"
                     /locus_tag="KLK84_003572"
     CDS             4232..4627
                     /gene="frdC"
                     /locus_tag="KLK84_003572"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBE2010750.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4638..4997
                     /gene="frdD"
                     /locus_tag="KLK84_003573"
     CDS             4638..4997
                     /gene="frdD"
                     /locus_tag="KLK84_003573"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBE2010751.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5060..6193
                     /locus_tag="KLK84_003574"
     CDS             5060..6193
                     /locus_tag="KLK84_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBE2010752.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6282..6815
                     /gene="blc"
                     /locus_tag="KLK84_003575"
     CDS             6282..6815
                     /gene="blc"
                     /locus_tag="KLK84_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HBE2010753.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6812..7129)
                     /gene="sugE"
                     /locus_tag="KLK84_003576"
     CDS             complement(6812..7129)
                     /gene="sugE"
                     /locus_tag="KLK84_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBE2010754.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7305..7451)
                     /gene="ecnB"
                     /locus_tag="KLK84_003577"
     CDS             complement(7305..7451)
                     /gene="ecnB"
                     /locus_tag="KLK84_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HBE2010755.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7562..7687)
                     /gene="ecnA"
                     /locus_tag="KLK84_003578"
     CDS             complement(7562..7687)
                     /gene="ecnA"
                     /locus_tag="KLK84_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HBE2010756.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7739..8305)
                     /gene="efp"
                     /locus_tag="KLK84_003579"
     CDS             complement(7739..8305)
                     /gene="efp"
                     /locus_tag="KLK84_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBE2010757.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8347..9375
                     /gene="epmB"
                     /locus_tag="KLK84_003580"
     CDS             8347..9375
                     /gene="epmB"
                     /locus_tag="KLK84_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HBE2010758.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2451 a         2473 c         2459 g         2261 t
ORIGIN      
        1 taaggcgaat ggaataaaga ataacaatgc agaaaatata taaaagggaa tcgcactata
       61 acccattaag taataagccg atgggctatt ggcaaatcca aatacggaag taaagatcat
      121 caatatgagt cctatcagac tcatcttttt tatcgtgtga ggcatgaaac catccttcat
      181 ttcagcgcgt gtataacacg tgcccactga actgcactgc gcaacattga gcaggcagcg
      241 taaaaaccaa tacagaattg atacttggat gatatcaaat atccagtctt aaattgtggc
      301 ctttaacgga aaaatgaaaa catatgctgc agaatggcgc ttatcacgcc attcttcgct
      361 gttaattcag taatttttca gaattatgcc cggtcaacgc taaaggcgat gacttcagcc
      421 agtgtctccg cgcccagcgc caacatcacc agacgatcaa cacctaatgc cacgccggaa
      481 cagtcaggca taccaacttt caaggcttca atcagattct ggtcaatggg gtgctgcggc
      541 aaaccgcgcg ccgcgcgctt acggttatct tgttcaaagc gttgttgctg ctcacgggca
      601 tccgttaatt catggaaacc attcgccagc tcaatacctt tataataaac ctcaaagcgt
      661 tcagcgaccc gatgatcttc ggtactgatt tgcgccagtg atgcctggct ggctggaaag
      721 tggtacacaa aggtcggttt ttctttacca atatttggct ctacgccaaa ggtaaacagc
      781 aattgtagca acgtgtcgcg gtcttcttcg gtatcagcaa cattgctcaa atccagtttc
      841 gctgcgactt cccgcagttg cgttttgtcg gcagagagcg ggtcaatttc cagataacgc
      901 aagaaagctt gttgataaga aaggctttct gctgccgggc agtccagcac ctgttgtaag
      961 agatcgtcca cctcgttcat caaccggtac atatcatagt gcggtcgata ccactccagc
     1021 atagtgaact cagggttgtg ataacgcccc atctcttcat tacggaagct gcggcacagc
     1081 tggaataccg gcccacaacc agcaaccagc aggcgtttca tatggtattc cgggctggtc
     1141 attaaccaga gattcatccc ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg
     1201 accaaatgaa tatcggttac cgtcgcctgg ctcatacaag gcgtctccac ctccagcact
     1261 ccacgatcgg caaagaaacg acggatctcc gccataatcg ccgcgcgttt taataagtta
     1321 ggaatggatg cgctcggctg ccaggatgcc gtttcgctca tagttaaatc tccagttttt
     1381 gacaagggca cgaagtctac tcgcaacgcg acggcgagac aaattttacg caggaatcaa
     1441 acagcggtgg gcagtgacta aaaaaagcac gatctgatgg tttagtaatt aaattaatca
     1501 tcttcagtga taatttagcc ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga
     1561 cttatccatc agactatact gttgtaccta taaaggagca gtggaatagc gttcgcagac
     1621 cgtaactttc aggcacttac cctgaagtac ggggctgtgg gataaaaaca atctggagga
     1681 atgtcgtgca aacctttcaa gccgatcttg ccattgtagg cgccggtggc gcgggattac
     1741 gtgctgcaat tgctgccgcg caggcaaatc caaatgcaaa aatcgcacta atctcaaaag
     1801 tatacccgat gcgtagccat accgttgctg cagaaggggg ctccgccgct gtcgcgcagg
     1861 atcatgacag cttcgaatat cactttcacg atacagtagc gggtggcgac tggttgtgtg
     1921 agcaggatgt cgtggattat ttcgtccacc actgcccaac cgaaatgacc caactggaac
     1981 tgtggggatg cccatggagc cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg
     2041 gcatgaaaat cgagcgcacc tggttcgccg ccgataagac cggcttccat atgctgcaca
     2101 cgctgttcca gacctctctg caattcccgc agatccagcg ttttgacgaa catttcgtgc
     2161 tggatattct ggttgatgat ggtcatgttc gcggcctggt agcaataaac atgatggaag
     2221 gcacgttggt gcagatccgt gctaacgcgg tcgttatggc taccggcggt gcgggtcgcg
     2281 tttatcgtta caacaccaac ggcggcatcg ttaccggtga cggtatgggt atggcgctaa
     2341 gccacggcgt tccgctgcgt gacatggaat tcgttcagta tcacccaacc ggtctgccag
     2401 gttccggtat cctgatgacc gaaggctgcc gtggtgaagg cggtattctg gtcaacaaaa
     2461 atggctaccg ttatctgcaa gattacggca tgggcccgga aactccgctg ggcgagccga
     2521 aaaacaaata tatggaactg ggtccacgcg acaaagtttc tcaggccttc tggcacgaat
     2581 ggcgtaaagg caacaccatc cccacgccgc gtggtgatgt ggtttatctc gacctgcgtc
     2641 acctcggcga gaaaaaactg catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt
     2701 acgttggcgt cgatccggtt aaagaaccga ttccggtacg tccgactgca cactacacca
     2761 tgggcggtat cgaaaccgat cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg
     2821 gtgaatgttc ctctgttggt ctgcacggtg caaaccgtct gggctccaac tccctggcgg
     2881 aactggtggt cttcggccgt ctggccggtg aacaagcgac agagcgtgca gcaactgccg
     2941 gtaatggcaa cgaagcggca attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag
     3001 atctagttaa ccaggatggc ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg
     3061 caatggaaga aggttgcggt atctaccgta cgccggaact gatgcagaaa accatcgaca
     3121 agctggcaga gctgcaggaa cgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt
     3181 tcaacaccga cctgctctac accattgaac tgagccacgg tctgaacgtt gctgaatgta
     3241 tggcgcactc cgcaatggca cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag
     3301 gttgcaccga gcgtgacgac gtcaactttc tcaaacacac cctcgccttc cgcgatgctg
     3361 atggcacgac tcgcctggag tacagcgacg tgaagattac tacgctgccg ccagctaaac
     3421 gcgtttacgg tggcgaagcg gatgcagccg ataaggcgga agcagccaat aataaggaga
     3481 aggcgaatgg ctgagatgaa aaacctgaaa attgaggtgg tgcgctataa cccggaagtc
     3541 gataccgcac cgcatagcgc attctatgaa gtgccttatg acgcaactac ctcattactg
     3601 gatgcgctgg gctacatcaa agacaacctg gcaccggacc tgagttaccg ctggtcctgc
     3661 cgtatggcga tttgtggttc ctgcggcatg atggttaaca acgtgccaaa actggcatgt
     3721 aaaaccttcc tgcgtgatta caccgacggt atgaaggttg aagcgttagc taacttcccg
     3781 attgaacgcg atctggtggt cgatatgacc cacttcatcg aaagtctgga agcgatcaaa
     3841 ccgtacatca tcggcaactc ccgcaccgcg gatcagggta ctaacatcca gaccccggcg
     3901 cagatggcaa agtatcacca gttctccggt tgcatcaact gtggtctgtg ctacgccgcg
     3961 tgcccgcagt ttggcctgaa cccagagttc atcggtccgg ctgccattac gctggcgcat
     4021 cgttataacg aagatagccg cgaccacggt aagaaggagc gtatggcgca gttgaacagc
     4081 cagaacggtg tatggagctg tactttcgtg ggctattgct ccgaagtctg cccgaaacac
     4141 gtcgatccgg ctgcggccat tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc
     4201 gcgaccctga aaccacgcta aggagtgcaa catgacgact aaacgtaaac cgtatgtacg
     4261 gccaatgacg tccacctggt ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga
     4321 aggcacggcg gttccggctg tgtggttcag cattgaactg attttcgggc tgtttgccct
     4381 gaaaaatggc ccggaagcct gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt
     4441 gatcattaac ctgatcactc tgacggcagc tctgctgcac accaaaacct ggtttgaact
     4501 ggcaccgaaa gcggccaata tcattgtaaa agacgaaaaa atgggaccag agccaattat
     4561 caaaagtctc tgggcggtaa ctgtggttgc caccatcgta atcctgtttg ttgccctgta
     4621 ctggtaagga gcctgagatg attaatccaa atccaaagcg ttctgacgaa ccggtattct
     4681 ggggcctctt cggggccggt ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc
     4741 tggtgggtat tctgctgcca ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg
     4801 ttctggcgtt cgcgcagagc ttcattggtc gcgtattcct gttcctgatg atcgttctgc
     4861 cgctgtggtg tggtttacac cgtatgcacc acgcgatgca cgatctgaaa atccacgtgc
     4921 ctgcgggcaa atgggttttc tacggtctgg ctgctatcct gacagttgtc acgctgattg
     4981 gtgtcgttac aatctaacgc atcgccaatg taaatccggc ccgcctatgg cgggccgttt
     5041 tgtatggaaa ccagacctta tgttcaaaac gacgctctgc gccttattaa ttaccgcctc
     5101 ttactccaca tttgctgccc ctcaacaaat caacgatatt gtgcatcgca caattacccc
     5161 gcttatagag caacaaaaga tccccggtat ggcggtggcg gtaatttatc agggtaaacc
     5221 ttattacttt acctggggct atgcggacat cgccaaaaag cagcccgtca cacagcaaac
     5281 gttgtttgag ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg gcgacgctat
     5341 tgctcgaggg gaaatcaagt taagcgatcc cgcaacaaaa tactggcctg aacttaccgc
     5401 taaacagtgg aatgggatca cactattaca tcttgcgacc tacaccgctg gcggcctgcc
     5461 attgcaggtg ccggatgaag tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg
     5521 gcagcctgca tgggctccag gaacacaacg tctgtatgcc aactccagta tcggtttgtt
     5581 cggcgcactg gctgtgaagc cgtctggttt gagttttgag caggcgatgc aaactcgtgt
     5641 cttccagcca ctcaaactca accatacgtg gattaatgta cctcccccag aagaaaagaa
     5701 ttacgcctgg ggatatcgcg aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc
     5761 tgaagcttat ggtgtgaagt cgaccattga agatatggcc cgctgggtgc aaagcaattt
     5821 accccccctt gatatcaatg agaaaacgct tcaacaaggg atacaactgg cacaatctcg
     5881 ctactggcaa accggcgata tgtatcaggg cctgggctgg gaaatgctgg actggccggt
     5941 caatcctgac agcatcatta acggcagtga caataaaatt gcactggcag cacgcccggt
     6001 aaaagcgatt aagcccccaa ctcctgcagt acgcgcatca tgggtacata aaacgggggc
     6061 gaccggcgga tttggtagct atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat
     6121 gctggctaac aaaaactatc ccaatccagc gagagtcgcc gccgcctgtc agattctcaa
     6181 cgctctacag taaaatttca tcgggtccga attttcggac cttttctgcg cttttccttg
     6241 ctgtcatcta cacttagaaa aaaaccagta aggaaacatt tatgcgcctg ctccctctcg
     6301 ttgccgcagc gacagctgca tttctggtcg ttgcctgtag ttttcctacg ccgccgcgtg
     6361 gcgtgaccgt agtgaataat ttcgacgcca aacgttatct cggtacctgg tatgagattg
     6421 cccgttttga tcaccgtttt gaacgtggac tggaaaaagt caccgcaaca tacagcctgc
     6481 gtgatgacgg cggcctgaat gtcattaata aaggctataa ccctgacaga ggaatgtggc
     6541 agcagagtga agggaaagcg tactttaccg gcgcaccaac tcgcgctgcg ctgaaagtgt
     6601 cattctttgg tcctttctat ggcggttata acgttattgc actcgatcgg gaataccgcc
     6661 atgcgctggt ttgcggcccg gaccgcgact acctgtggat actctcccgc acgccaacca
     6721 tttctgacga agtgaaacag gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca
     6781 gtaagtttat ttgggtacag cagcccggta gttagtgagt gctgagtttc agaccaataa
     6841 tccccaatac gattaacgcc agactcgcca gacgcatcgg gttagcggac tcaccgagca
     6901 gcacaatgcc ggtgatggcc gcgccgactg cgccaatacc cgtccacacg gcataagccg
     6961 tccctaccgg taacgatttc atcgcccagg caagtagcgc catactgact atcatcgccg
     7021 tcacggtaat aacactcggc gtcaaacggc taaagccgtg ggtatatttc aggccaacgg
     7081 cccataccac ttccagcaga ccagcaataa ctaagataat ccaggacata tcaggctccg
     7141 gaaatttggg gccgtccccg gtgaaaagag gcgtttgcag gtcgtcctgc aaagctgatg
     7201 gttgaacgga tattttgcca ggtggcaggg gaatttcaac tattaccttt atgacgatcg
     7261 cagaaaaaac aaacggcacg acactgctgt cgtaccgtta ttgcttattg ctgcgctttc
     7321 gttgctgcgc cagaaatcgc gttaccgcca tcagaaatgt cttcaccaac gccacgcgtg
     7381 gtgttgcagg cagttaatac tgttgaaagc accagaacag aaaagatcgc tgcaattgtc
     7441 ttcttcacca taacgtcttc cttttaggta atgttattta tgtttgccta tagcaaatta
     7501 ataatagaca acatcgccaa aaatgatgga aatctgatga tttttcggaa gagaagaaaa
     7561 attagctggc agcgcgagag atggagttac cgagatgttt gatgtcttcg ccgaaaccgc
     7621 aagcggtgtt acagcccgtg agcagcgtgc tggcaagcaa aacaagaacg ataaggcgtt
     7681 tcatcatctc tgacctgaat aagtgatggt gcagcctgca ggccgcacca caaccgcatt
     7741 acttcacgcg agagacgtat tcaccagagc gggtatccac tttgatgact tcgccgattt
     7801 gtacgaacag cggaacttta accacagcgc cagtagacag ggtagccggt ttgccgccag
     7861 tacctgcggt atcacctttc aggcccggat cggtatcaac gatttccagt tcaacgaagt
     7921 tcggcggagt aacggagatc ggctgaccat tccacagagt tacgatacac tctgcctgat
     7981 ccagcagcca tttagcattg tcgccaattg cttttgcatc agcagacagc tgctcgaaag
     8041 tttcgttgtt catgaagtgc cagaactcac cgtcgttgta caggtaagtc aggttcatat
     8101 cgacaacatc agcgccttca gcggaatcag tagatttgaa ggttttttct acgcgagtac
     8161 cggtcagcag acgacgcagt ttaacgcgag caaatgcctg gcctttaccc ggttttacga
     8221 attcactcgc ttcaaccgcg taaggttcgc cgtctaacat gattttaaga ccagcacgaa
     8281 aatcgttgct atagtacgtt gccataaggc cctctgaaat ttgttaattg gtagctaagc
     8341 cacaaaatgg cgcatattgt aaccctaaat accccatcca gagaagattg gttaacgcaa
     8401 cttgccgatg ttgtgaccga tcctgatgaa cttctgcgtc ttttgaatat agacgcggac
     8461 gaaaaactgt tggccggacg cagcgccaaa aagctgtttg ccctgcgtgt gccccgctca
     8521 tttatcgatc gcatggagaa aggcaatccg gacgatcctc ttttgcgtca ggtacttacc
     8581 tcgcaagatg agtttgtcgt cgcgtccgga ttctccaccg acccactgga agaacagcac
     8641 agcgtagtgc ctggtttgtt gcataaatac cacaaccggg cgcttttgct ggtcaaaggc
     8701 ggctgcgcgg taaattgccg ctattgcttc cgtcgccact tcccctatgc cgaaaatcag
     8761 ggcaacaagc gtaactggca gactgcactt gagtatgttg ctgcgcatcc ggaactggac
     8821 gagatgattt tctccggcgg cgatccgctg atggcgaaag agcacgagct ggactggttg
     8881 ctcacacaac tggaagccat cccgcatatc aaacgtctgc ggattcacag ccgtctgccg
     8941 attgtgatcc cggcacgtat caccgaggcg ctggttgaac gctttgcccg ttctacgctg
     9001 caaatcttgc tggtgaatca catcaatcat gccaatgagg tagatgaaac gttccgtcag
     9061 gcgatggcta aattgcgccg tgtcggtgtc accctgctta accagagcgt tctgttacgt
     9121 ggtgtgaacg ataacgcaca aacgttggca aacctgagta atgcgttgtt cgatgccggc
     9181 gtgatgccgt attacctgca tgtgctagat aaagtacagg gcgcggcgca ttttatggtg
     9241 agtgatgacg aagcacggca gattatgcgt gagttgctga cattggtgtc gggatatctg
     9301 gtgccgaaac tggcgcgaga aattggcggc gaacccagca aaacgccgct ggatctccag
     9361 ctacgccagc agtaactgat taaacaggga agttaaacat taaaattgcc tgatgcgcta
     9421 cgcttatcag gcctacatcg tgcattgaaa ttgaatggat ttgcaatgaa ctgtaggccg
     9481 gataaggcgt ttacgccgca tccggcaaga gcaacaaacg caagtacagc aacctgtcat
     9541 taaactgaga aatttaatct ttgcacgttg cataaccttt aattattcat atgcattgct
     9601 gttgttactt tttatatagc taacaccaat aattaacctt tctt
//
LOCUS       DAEEUE010000131         9625 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.131, whole genome shotgun sequence.
ACCESSION   DAEEUE010000131 DAEEUE010000000
VERSION     DAEEUE010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9625
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.131"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(16..999)
                     /gene="kdgT"
                     /locus_tag="KLK84_003581"
     CDS             complement(16..999)
                     /gene="kdgT"
                     /locus_tag="KLK84_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HBE2010759.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="KLK84_003582"
     CDS             complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="KLK84_003582"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HBE2010760.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2164..3198
                     /gene="rhaT"
                     /locus_tag="KLK84_003583"
                     /pseudo
     CDS             2164..3198
                     /gene="rhaT"
                     /locus_tag="KLK84_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3195..4061)
                     /gene="rhaR"
                     /locus_tag="KLK84_003584"
     CDS             complement(3195..4061)
                     /gene="rhaR"
                     /locus_tag="KLK84_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HBE2010761.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="KLK84_003585"
     CDS             complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="KLK84_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HBE2010762.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5241..6710
                     /gene="rhaB"
                     /locus_tag="KLK84_003586"
     CDS             5241..6710
                     /gene="rhaB"
                     /locus_tag="KLK84_003586"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBE2010763.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6707..7966
                     /gene="rhaA"
                     /locus_tag="KLK84_003587"
     CDS             6707..7966
                     /gene="rhaA"
                     /locus_tag="KLK84_003587"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBE2010764.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8208..9032
                     /gene="rhaD"
                     /locus_tag="KLK84_003588"
                     /pseudo
     CDS             8208..9032
                     /gene="rhaD"
                     /locus_tag="KLK84_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            9042..9356
                     /gene="rhaM"
                     /locus_tag="KLK84_003589"
     CDS             9042..9356
                     /gene="rhaM"
                     /locus_tag="KLK84_003589"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBE2010765.1"
                     /translation="MIRKAFVMQVNPDAHEEYQSRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         2352 a         2515 c         2489 g         2269 t
ORIGIN      
        1 attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt
       61 gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc
      121 cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc
      181 aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt
      241 gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat
      301 tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat
      361 ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa
      421 ttctcgtaat tcagggtcaa gattccccag ggcaaagccc accaggaacg gtaatactgc
      481 gccgacgaaa acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt
      541 catgagcggc ccggactcca acgacatcaa cacaaatgcc ccagcttctt ctttcgtacc
      601 gtactgctgc atgatggaag cgtaaagtcc gccgttggtc atatccatcg ccgccaccag
      661 cgccagcgtt gaaagtccgg caaagaatcc aacttcaaca ccatgttccg gaataatgcg
      721 cgaggcaatt gccgcaacca cccacgcgac ggcaatttta gttaccacca gcgtaccgga
      781 tttacgcagt accgttcccg tcgcgcttaa ttttattgac gcccccatgc aaaaaaacca
      841 caccgccaga atgggcaccg taccggtaat catcccgttg gtgaatgatc caaaatattt
      901 ccccgccccc ggcgagaagg tgtggcacag tgcgccaagg aatagcggga cgagcatcat
      961 cccccccggg attttctcaa tcgagcgttt tatctgcatt tccatcacct gtcggcatat
     1021 ttttaagggc tgatgcgtaa ttcctcaatg gagcatcagt gttgatggaa acgaagatag
     1081 acattcattt aatcaataaa agtgatcaaa atcacatcaa ttgaaacgct gttttatttt
     1141 tataagacca ttgtttttat ttttaaattg atcacaaaaa aaccaccgcc gttggcgatg
     1201 gttcattttt taagctgata tgcggcctat acgcctcatt gcagcaggcg gcaaatgatt
     1261 attttttcgc cgcaaaacgt gccgctgctt cgtcccagtt caccacgttc cagaactctt
     1321 tgatgtagtc cggacggcgg ttctggaatt tcaggtagta agcatgttcc cacacatcca
     1381 ggcccagaat cgggaagccg gaagcgccag aaatagcttc acccatcagc ggagaatcct
     1441 ggttagcagt agaaaccacc gccagtttat cgcctttcag caccagccat gcccagccgg
     1501 aaccaaagcg ggaagctgcc gctttttcaa attctgcttt gaagttatca acggagccga
     1561 agtcacgttc gatagccgct ttcaggtcac cctgcagggt ggtgcctttt ttcagacctt
     1621 tccagaacag gctgtggtta gcgtgaccgc cagcgttgtt gcgcagtacg gttttcttgt
     1681 ctgctggcag ctggtccagt ttggtgatca gctcttcaac cggcaggttg gcaaattctg
     1741 gcaggctttc cagcgccgcg ttggcgttgt ttacgtaggt ctgatggtgt ttggtgtggt
     1801 ggatttccat ggtctgctta tcgaagtgcg gttccagggc atcgtaagca tacggcaggg
     1861 atggcagggt atagctcata ttcatctcca gtattgtcgg gcggccgatt gttaatgccg
     1921 cgtaagcagt tggttcatta tagttaatta aatgatattg aaaatgatta tcaatgccgt
     1981 acttttcgta agggtatggt tttgcaggaa aatgcccgag atgtgaagca aatcacccac
     2041 ttaatgccgt gattgccagt aaatcgacaa cggcggcaac aggcgaaagg ttaatcgaca
     2101 gcacgatttt tacactcatc tcgtcggaga tgtgaagcga cgaaaaatga tgaggataag
     2161 aagatgagta acgcgattac gatggggata ttttggcatt tgatcggcgc ggccagtgca
     2221 gcctgttttt acgctccgtt caaaaaagta aaaaaatggt catgggaaac catgtggtca
     2281 gtcggtggga ttgtttcgtg gattattctg ccgtgggcca tcagcgccct gttactaccg
     2341 gatttctggg cgtattacag ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc
     2401 ggcgctatgt gggggatcgg taatatcaac tatggcctga ccatgcgtta tctcggcatg
     2461 tcgatgggaa ttggcatcgc cattggcatt acgttgattg tcggtacgct gatgacgcca
     2521 attatcaacg gcaatttcga tgtgctgatt aacaccgaag gcggacgcat gacgttactc
     2581 ggcgttctgg tggcgctgat tggcgtaggg attgtaactc gagccgggca gttgaaagag
     2641 cgcaagatgg gcattaaagc cgaagagttc aatctgaaaa aagggctggt gctggcggtg
     2701 atgtgcggca ttttctctgc cgggatgtcc tttgcgatga acgccgcaaa accgatgcat
     2761 gaagccgctg ccgcacttgg cgtcgatcca ctgtatgttg ctctgccaag ctatgttgtc
     2821 atcatgggcg gcggcgcgat cattaacctc ggtttctgtt ttattcgtct ggcaaaagtg
     2881 aaggatttgt cgctaaaagc cgacttctcg ctggcaaaac cgctgatcat tcacaatgtg
     2941 ttactctcgg cactgggcgg gttgatgtgg tatctgcaat tctttttcta tgcctagggc
     3001 cacgcccgca ttccggcgca gtatgactac atcagttgga tgctgcatat gagtttctat
     3061 gtattgtgcg gcggtatcgt cgggctggtg ctgaaagagt ggaacaatac aggacgccgt
     3121 ccggtaacgg tgttgagcct cggttgtgtg gtgattattg tcgccgctaa catcgtcggc
     3181 atcggcatgg cgaattaatc tttctgcgaa ttgagatgac gccactggct gggcgtcatc
     3241 ccggtttccc gggtaaacac caccgaaaaa tagttactat cttcaaagcc acattcggtc
     3301 gaaatatcac tgattaacag gcggctatgc tggagaagat attgcgcatg acacactctg
     3361 acctgccgca gatattgatt gatggtcatt ccagtctgct ggcgaaattg ctgacgcaaa
     3421 acgcgctcac tgcacgatgc ctcatcacaa aatttatcca gcgcaaaggg acttttcagg
     3481 ctagccgcca gccgggtaat cagcttatcc agcaacgttt cgctggatgt tggcggcaac
     3541 gaatcactgg tgtaacgatg gcgattcagc aacatcacca actgcccgaa cagcaactca
     3601 gccatttcgt tagcaaacgg cacatgctga ctactttcat gctcaagctg accgataacc
     3661 tgccgcgcct gcgccatccc cacgctacct aagcgccagt gtggttgccc tgcgctggcg
     3721 ctaaatcccg gaatcgctcc ctgccagtca agattcagct tcagccgctc cgggcaataa
     3781 ataatattct gcaaaaccag atcgttaacg gaagcgtagg agtgtttatc gtcagcatga
     3841 atataaaaga gatcgccacg ggtaatgcga taaggacgat cgttgagtac atgcagacca
     3901 ttaccgcgcc agacaatcac cagctcacaa aaatcatgtg tatgttcggc aaagacatct
     3961 tgcggataac ggtcagccac agcgactgcc tgctggtcgc tggcaaaaaa atcatctttg
     4021 agaagtttta actgatgcgc caccgtggct acctcggcca gagaacgaag ttgattattc
     4081 gcaatatggc gtacaaatac gttgagaaga ttcgcgttat tgcagaaagc catcccgtcc
     4141 ctggcgaata tcacgcggtg accagttaaa ctctcggcga aaaagcgtcg aaaagtggtt
     4201 actgtcgctg aatccacagc gataggcgat gtcagtgacg ctggcctcgc tgtggcgtag
     4261 tagatgtcgg gctttcatca gtcgcaggcg gttcaggtat cgctgaggcg tcagtcccgt
     4321 ttgctgctta agctgccgat gtagcgtacg cagtgaaaga gaaaattgat ccgccacggc
     4381 atcccaattc acctcatcgg caaaatggtc ctccagccag gccagaagca agttgagacg
     4441 tgatgcgctg ttttccaggt tctcctgcaa actgctttta cgcagcaaga gcagtaattg
     4501 cataaacaag atctcgcgac tggcggtcga gggtaaatca ttttcccctt cctgctgttc
     4561 catctgtgca accagctgtc gcacctgctg caatacgctg tggttaacgc gccagtgaga
     4621 cggatactgc ccatccagct cttgtggcag caactgattc agcccggcga gaaactgaaa
     4681 tcgatccggc gagcgataca gcacattggt cagacacaga ttatcggtat gttcatacag
     4741 atgccgatca tgatcgcgta cgaaacagac cgtgccaccg gtgatggtat agggctgccc
     4801 attaaacaca tgaatacccg tgccatgttc gacaatcaca atttcatgaa aatcatgatg
     4861 atgttcagga aaatccgcct gcgggagccg gggttctatc gccacggacg cgttacaaga
     4921 cggaaaaaaa tccacactat gtaatacggt catactggcc tcctgatgtc gtcaacacgg
     4981 cgaaatagta atcacgaggt caggttctta ccttaaattt tcgacggaaa actacgtaaa
     5041 aaacgtcgat ttttcaagat acagcgtgaa ttttcaggaa atgcggtgag catcacatca
     5101 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc
     5161 attttcctgt cagtaacgag aaggtcgcga attcaggcgc tttttagact ggtcgtaatg
     5221 aaattcagca ggatcacatt atgacctttc gcaattgtgt cgccgtcgat ctcggcgcat
     5281 ccagtgggcg cgtgatgctg gcgcgttacg agcgtgaatg ccgcagcctg acgctgcgcg
     5341 aaatccatcg ttttaacaat gggctgcata gccaaaacgg ttatgtcacc tgggatgtgg
     5401 atagcctgga aagtgccatt cgccttggat taaacaaggt gtgcgaggaa gggattcgta
     5461 tcgatagcat tgggattgat acctggggcg tggactttgt gctgctcgac caacagggtc
     5521 agcgtgtggg cctgcccgtt gcttatcgcg atagccgcac caatggcctg atgccgcagg
     5581 cacaacagca actcggcaaa cgcgatattt atcagcgtag cggcatccag tttctaccct
     5641 tcaatacgct ttatcagttg cgtgcgctga cggagcaaca acctgaactt attccacaca
     5701 ttgctcacgc tctgctgatg ccggattact tcagttatcg cctgaccggc aagatgaact
     5761 gggaatatac caatgccacc accacacaac tggtcaatat caatagcgac gactgggacg
     5821 agtcgctact ggcgtggagc ggggccaaca aagcctggtt tggtcgcccg acgcatccgg
     5881 gtaatgtcat tgggcactgg atttgcccgc agggtaatga gattccggtg gtcgccgttg
     5941 ccagccatga taccgccagc gcggttatcg cctcgccgtt aaacggttca cgcgccgctt
     6001 atctctcttc tggcacctgg tcattgatgg gcttcgaaag ccagacgcca tttaccaatg
     6061 acacggcgct ggcagccaac atcaccaatg aaggcggggc ggaaggtcgc tatcgggtgc
     6121 tgaaaaatat tatgggctta tggctgcttc agcgagtgct tcaggaacgg caaatcaacg
     6181 atctcccggc gcttatcgcc gcgacacagg cacttccggc ctgccgcttc atcatcaatc
     6241 ccaatgacga tcgctttatt aatcctgacg agatgtgcag cgaaattcag gctgcgtgtc
     6301 gggaaacggc gcaaccgatc ccgggaagtg atgctgaact ggcgcgctgt attttcgaca
     6361 gtctggcgct gctgtatgcc gatgtgttgc atgagctggc gcagctgcgc ggtgaagatt
     6421 tctcgcaact gcatattgtc ggcggcggct gccagaacac gctgctcaac cagctatgcg
     6481 ccgatgcctg cggtattcgg gtgatcgccg ggcctgttga agcctcaacg ctcggcaata
     6541 tcggcatcga gttaatgacg ctggatgaac tcaacaatgt ggatgatttc cgtcaggtcg
     6601 tcagcaccac cgcgaatctg accaccttta cccctaatcc tgacagtgaa attgcccact
     6661 atgtggcgca gattcactct acacgacaga caaaggagct ttgcgcatga ccactcaact
     6721 ggaacaggcc tgggagctgg cgaaacagcg tttcgcggcg gtggggattg atgtcgagga
     6781 ggcgctgcgc caacttgatc gtttacccgt ttcaatgcac tgctggcagg gcgatgatgt
     6841 ttccggtttt gaaaacccgg aaggttcgct gaccgggggg attcaggcca caggcaatta
     6901 tccgggcaaa gcgcgtaatg ccagtgagct acgtgccgat ctggaacagg ctatgcggct
     6961 gattccgggg ccgaaacggc ttaatttaca tgccatctat ctggaatcag atacgccagt
     7021 ctcgcgcgac cagatcaaac cagagcactt caaaaactgg gttgaatggg cgaaagccaa
     7081 tcagctcggt ctggatttta acccctcctg cttttcgcat ccgctaagcg ccgatggctt
     7141 tacgctctcg catcccgacg acagcattcg ccagttctgg attgatcact gcaaagccag
     7201 ccgtcgcgtt tcggcctatt ttggcgagca actcggcaca ccatcggtga tgaacatctg
     7261 gatcccggat ggtatgaaag atatcaccgt tgaccgtctc gccccgcgtc agcgtctgct
     7321 ggcagcactg gatgaggtga tcagcgagaa gctaaaccct gcgcaccata tcgacgccgt
     7381 tgagagcaaa ttgtttggca ttggcgcaga gagctacacg gttggctcca atgagtttta
     7441 catggggtat gccaccagcc gccagactgc gctgtgcctg gacgccgggc acttccaccc
     7501 gactgaagtg atttccgaca agatttccgc cgccatgctg tatgtaccgc agttgctgct
     7561 gcacgtcagc cgtccggttc gctgggacag cgatcacgta gtgctgctgg atgatgaaac
     7621 ccaggcaatt gccagtgaga ttgtgcgtca cgatctgttt gaccgggtgc atatcggcct
     7681 tgacttcttc gatgcctcta tcaaccgcat tgccgcgtgg gtcattggta cacgcaatat
     7741 gaaaaaagcc ctgctgcgtg cgttgctgga acctaccgct gagctgcgca agctggaagc
     7801 ggcgggcgat tacactgcgc gtctggcact gctggaagag cagaaatcgt tgccgcggca
     7861 ggcggtctgg gaaatgtatt gccaacgtca cgatacgcca gcaggtagcg aatggctgga
     7921 gagcgtgcgg gcttatgaga aagcgatttt gagccagcgt gggtaaacac tgccggatgc
     7981 ggcacgagcg ccttatccgg cctacgggtc gaatgtccat gcgggctgtc accctcaccc
     8041 cggccctctc cctgaaaggg cgagggagaa aaacatagcc tcgtgcggca acggagctga
     8101 acaccgctcc ggacagtccc ctcgccccta cggggagagg gttagggtga ggggaacaag
     8161 ccgcaccatg tctaaacaag gcaacctaat ttacaggaac acagaacatg caaaacatta
     8221 cccagtcctg gtttgtccag ggaatgatca aagccaccac cgatgcctag ctgaaaggct
     8281 gggacgagcg caacggcggc aacctgacgc tgcgcctgga tgacgccgat atcgcgcttt
     8341 atcacgacaa cttccaccca caaccgcgct atattccgct cagccaaccc atgcctttac
     8401 tggcaaatac gccgtttatt gtcaccggct ccggcaaatt cttccgtaac gtccagctcg
     8461 atcctgcagc taacttaggc gtcgtaaaag tcgacagcga tggcgcgggc taccatattc
     8521 tctgggggtt aaccaacgaa gccgttccaa cttctgaact tccggctcac tttctctccc
     8581 actgcgagcg cattaaagcc accaacggta aagatcgggt gatcatgcac tgccacgcca
     8641 ccaacctgat cgccctcacc tatgtactgg aaaacgacac cgcggtcttt actcgccaac
     8701 tgtgggaagg cagcaccgaa tgtctggtag tattcccgga tggcgttggc attctgccgt
     8761 ggatggtgcc cggcacggac gaaatcggcc aggcgaccgc acaagagatg caaaaacatt
     8821 cgctggtgtt gtggcccttc cacggtgtgt tcggttccgg accgacgctg gatgaagcct
     8881 ttggtttaat cgacaccgca gaaaaatcag cacaagtatt agtgaaggtt tattcgatgg
     8941 gcggcatgaa acagaccatc agccgtgaag agttgatagc gctcggcaag cgcttcggcg
     9001 ttacaccact agccagtgcg ctggcgctgt aaggagcaaa catgatccgc aaagcctttg
     9061 tcatgcaggt aaaccccgac gcccacgaag agtatcagag tcggcataat cccatctggc
     9121 cggaactgga agcagtgttg aaatctcacg gtgtgcataa ctacgccatt tacctcgaca
     9181 aagcgcgtaa tctgctgttt gccatggtag agattgaatc tgaagaacgg tggaatgcgg
     9241 tagccagtac tgatgtttgc caacgttggt ggaaatatat gaccgatgtt atgcccgcta
     9301 acccagataa cagcccggtg agtagcgagc tgcaagaagt gttttacctg ccgtaattgc
     9361 ccaatgtgcc ggagatgcct ccggcacaat attttaaatt tcgatttgaa aaattaaaca
     9421 tattacatct cccggtaagt tatatttccc tgatacattg tgagtaaatc acaaaaataa
     9481 tgaataaccc attaatgatt catgtggttt atttaaataa ccattatgtg cattactccg
     9541 caaatctgac ctttcactct gtctcaaatt gtcattgttt accgacatat cgcctctcat
     9601 cacttaatcg ctccaccatt acaat
//
LOCUS       DAEEUE010000132         9499 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.132, whole genome shotgun sequence.
ACCESSION   DAEEUE010000132 DAEEUE010000000
VERSION     DAEEUE010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9499
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.132"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            229..768
                     /locus_tag="KLK84_003590"
     CDS             229..768
                     /locus_tag="KLK84_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBE2010766.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSETWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGRAAGAQTQPEEGQQFSGQPQPEPQSEAGTKKGGAKTKGRGRKAAQPE
                     PQPQPPEGEDYGFSDDIPF"
     gene            831..1064
                     /locus_tag="KLK84_003591"
     CDS             831..1064
                     /locus_tag="KLK84_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HBE2010767.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIEDDHGEQFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            1130..3088
                     /locus_tag="KLK84_003592"
     CDS             1130..3088
                     /locus_tag="KLK84_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBE2010768.1"
                     /translation="MSVTESKAKTERKSSRKPAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSVESVSELAESIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERNILPADWPVRVKVILQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQTG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPDVRVIRNLITESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEFLREAEGWGWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLIDCMAKVRAWTPEMRAGGGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADRTEQVQEKASVGGISLPLLTKMSSERTLAVQAALMQQPD
                     KSLTLLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGVAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLETAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NCWVPGWMCAPHPQTDTTERTDNLADAA"
     gene            3143..3577
                     /gene="psiB"
                     /locus_tag="KLK84_003593"
     CDS             3143..3577
                     /gene="psiB"
                     /locus_tag="KLK84_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HBE2010769.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            3574..4293
                     /locus_tag="KLK84_003594"
     CDS             3574..4293
                     /locus_tag="KLK84_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HBE2010770.1"
                     /translation="MSARSQALVPLSTEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEAGMAARV
                     VGHAVREMERWMVPNKLREAA"
     gene            complement(4305..4493)
                     /locus_tag="KLK84_003595"
     CDS             complement(4305..4493)
                     /locus_tag="KLK84_003595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein sok"
                     /protein_id="HBE2010771.1"
                     /translation="MKINLLRGYLLSATQHGNKPPSRHEAESLKRRAHHSPYTCTLTT
                     LTFPENNPLIQTVHGKSV"
     gene            4573..4731
                     /locus_tag="KLK84_003596"
     CDS             4573..4731
                     /locus_tag="KLK84_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBE2010772.1"
                     /translation="MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAA
                     FMAYESGK"
     gene            5422..5628
                     /locus_tag="KLK84_003597"
     CDS             5422..5628
                     /locus_tag="KLK84_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBE2010773.1"
                     /translation="MYIPQNFLPRSRQKLLRSAPGQSFPSVAVMPLAAKVLKKVMVNT
                     GGNKRQVPAKRQNRGSRNSTDDGG"
     gene            5653..5940
                     /locus_tag="KLK84_003598"
     CDS             5653..5940
                     /locus_tag="KLK84_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010774.1"
                     /translation="MSTRNIHVNTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSQGVDKQFRHINIAEADHFKQFLRQIKRAGLDIRAIC"
     gene            6059..6880
                     /locus_tag="KLK84_003599"
     CDS             6059..6880
                     /locus_tag="KLK84_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBE2010775.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRREDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
     gene            complement(7177..7779)
                     /locus_tag="KLK84_003600"
     CDS             complement(7177..7779)
                     /locus_tag="KLK84_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HBE2010776.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFI
                     NEICQATDCFDLAGRDYKIDPDLLRAISWQESRYRVNAIGINPVTGYGSGLMQVDSQH
                     FNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVTWEAVGAYNAGFRKTERQNQ
                     RRLAYASDVYRIYTGIKSSKGIRLPATKKSLPEINSVQNN"
     gene            8110..8493
                     /locus_tag="KLK84_003601"
     CDS             8110..8493
                     /locus_tag="KLK84_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome protein TraM"
                     /protein_id="HBE2010777.1"
                     /translation="MAKVQAYVSDEIVYKINKIVERRRAEGAKSTDVSFSSISTMLLE
                     LGLRVYETQMERKESAFNQTEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMERFFPENDEG"
     gene            8627..9304
                     /locus_tag="KLK84_003602"
     CDS             8627..9304
                     /locus_tag="KLK84_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBE2010778.1"
                     /translation="MRERPLNSQSVNKYILNVQNIYRNSPVPVCVRNKKRKILYANGA
                     FIELFSKEDKPFSGESYVRLQVEIFLSSLELECQSLGHGSAFCRRFNFHGEIYQIRME
                     NVSFYNEESVVLWQINIFPDYPFFRVEKENYYHRDSYVQSVISNMTAKSLVVFCFYAL
                     GYKHINIAKELKITEVASKKRIKKINADINKRLGSFDLFRNKCIKTGVMYEILNVLSE
                     YMGVKKM"
     gene            9392..>9499
                     /locus_tag="KLK84_003603"
     CDS             9392..>9499
                     /locus_tag="KLK84_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraY domain-containing protein"
                     /protein_id="HBE2010779.1"
                     /translation="MRRRNARGGISRTVSVYLDEDTNNRLIRAKDRSGRS"
BASE COUNT         2405 a         2200 c         2614 g         2280 t
ORIGIN      
        1 acatcccgca aaactttttg cccagaagca ggcaaaaact tttgcggtct gcccccggac
       61 agtcgtttcc gtccgtggca gtgatgcccc atgcggcgaa ggttctcaaa aaggtgatgg
      121 tgaataccgg agggaacaaa aggcaggtcc ccgcaaaacg tcaaaaccgc ggctcccgaa
      181 actcaaaaga tgatgggggc tgaaaacatc aacagaagga ggcacatcat ggcagttcgt
      241 ggcattaaca aggtcattct cgtaggacgc ctgggaaaag atccggaagt ccgttacatc
      301 cccaacgggg gcgcagtggc aaacctgcag gtcgccacgt cggaaacctg gcgtgacaaa
      361 cagacagggg agatgcggga gcagacagaa tggcatcgcg tggtgctgtt cggcaagctc
      421 gcggaagtgg ctggcgaata tctgcgcaag ggcgcgcagg tctacatcga aggtcagctt
      481 cgcacccgta gctgggaaga taacggtatc acccgttacg tcaccgaaat tcttgttaag
      541 accacaggca ccatgcagat gctgggacgt gccgcaggtg ctcagactca gccggaagag
      601 gggcaacagt tcagcggtca gcctcagccg gaaccacagt cggaggccgg tacgaaaaaa
      661 ggtggcgcaa aaacgaaagg ccgtggacgt aaggccgcgc agcccgagcc tcagccgcaa
      721 ccgccggagg gggaggatta cgggttttca gacgatatcc cgttctgatc gggctgactg
      781 tgataaccgc cccgccccgt tctgtgcggg gcatcaccgg agagatgaga atgagcgaat
      841 atttcagaat acttcagggg ctgcctgacg gtccttttac ccgcaaacat gccgaagctg
      901 ttgccgcaca gtaccggaac gtctttatcg aggatgacca cggcgaacag tttcgcctgg
      961 ttgtccgtaa taacggtgca atggtctggc gtacctggaa ttttgaggac ggtgccgggt
     1021 actggatgaa ccatgtcatc agggatttcg ggattcttaa gtaagagagg tgccggacgg
     1081 ggacacgtcc ggcagacaat cattattacc gttttgggag gtactaacta tgtcagttac
     1141 agagtctaag gcaaaaacgg agcgtaaatc cagccgtaaa cctgcaaaaa cgcaggaaac
     1201 agtcctgtct gccctgctgg cgcagacgga ggaagtgagc gtgccgctgg cctcactgat
     1261 taaatcaccg ctgaatgtgc gcacggtgcc gtattctgtg gagtccgtca gcgaactggc
     1321 tgagtccatt aagggcgttg gcctgttgca gaatctggtc gtgcataccc tgcctggtga
     1381 ccgttacggt gtcgccgcag gtggtcgccg actggctgca ctcaacatgc tggcagagcg
     1441 caacatcctt ccggctgact ggcctgtacg cgtgaaggtt attctgcagg aactggcgac
     1501 tgccgcatca atgactgaaa acggtcatcg tcgggatatg caccctgccg aacagattgc
     1561 cggattccgc gcaatggcgc aggaaggcaa aacacctgcg cagactggcg acctgctggg
     1621 ctattcaccc cgccacgtcc agcgaatgct gaaactggca gaccttgcac ccgtcatcct
     1681 tgatgcgctg gcagaagacc gcatcaccac cgaacactgt caggcgctgg cgctggagaa
     1741 cgacaccgcg cgtcaggtgc aggtgtttga agccgcctgc cagtcaggat ggggcggtaa
     1801 accggatgtg cgggttatcc gcaacctgat taccgaaagt gaagtggcgg tggctgggaa
     1861 cagtaaattc cgcttcgtgg gggctgatgc cttctcgcca gacgaactgc gcaccgattt
     1921 gttcagcgac gacgagggtg gctatgtgga ctgcgtggcg ctcgatgccg ccctgctgga
     1981 aaaactccag gctgtcgcag agttccttcg ggaagccgaa ggctggggat ggtgtgccgg
     2041 acgcatggag cctgtaggtg agtgccgtga ggatgccgga acataccgct gtctgccgga
     2101 gccggaagcg gtgctgacgg aggcggaaga agaacgtctg aacgaactga tgacgcgtta
     2161 cgacgcgctg gaaaaccagt gtgaggaatc tgacctgctg gaagcagaaa tgaagctgat
     2221 tgactgcatg gcgaaggtca gagcgtggac gccggagatg cgtgccggag gtggagtggt
     2281 ggtgtcctgg cgttatggca acgtgtgtgt ccagcgaggt gtgcagttgc gcagtgagga
     2341 tgacgtggct gacgacgctg accgcacgga acaggtgcag gagaaagcgt cagtgggggg
     2401 aatcagtctg ccactgctga cgaaaatgtc ttccgaacgc acgctggcag tccaggcggc
     2461 gcttatgcag caaccggaca aatccctgac actgctggca tggacgctct gcctgaatgt
     2521 gtttggcagc ggagcgtaca gtaaaccggc acaaatcagc ctggaatgta aacattattc
     2581 gctgaccagc gatgcgccgt cagggaagga aggtgtcgca ttcatggcgc tgatggcaga
     2641 aaaagcccgt cttgctgccc tgttaccgga gggatggtca cgagacatga cgacattcct
     2701 gtcactcagt caggaggtgc tgttgtccct gctcagtttc tgcaccgcat gcagccttaa
     2761 cggtgtccag acccgtgagt gtggtcacac gtcacgcagt ccgcttgact cgctggaaac
     2821 cgctatcgga ttccacatgc gcgactggtg gcagccgaca aaagcaaact tcttcggaca
     2881 cctgaaaaag ccgcagatta tcgcagccct gaatgaggca ggactgtccg gtgctgcacg
     2941 ggatgcagag aagatgaaga aaggtgatgc ggctgaacat gcagagcacc atatgaaaga
     3001 caactgctgg gtgcctggct ggatgtgtgc accacatcca cagacggata ccactgaacg
     3061 caccgataac ctggctgatg ccgcctgata aacaaccaca ccgccccgcc ggagacgggg
     3121 cggcagcaag ggagataccg tgatgaaaac tgaactgacc ctgaatgtat tacagaccat
     3181 gagcgcacag gaatatgaag atatccgggc agccggaagt gatgaacgtc gtgagctgac
     3241 acacgccgtg atgcgggaac tggatgcgcc ggacaactgg atgatgaatg gcgagtatgg
     3301 cagtgagttc gggggctttt tccccgtcca ggttcgtttc acgccagccc acgaacgttt
     3361 ccacctggcg ttatgttcac cgggcgatgt ctctcaggtc tgggtgctgg ttctggtgaa
     3421 tgccggtggt gagccgtttg cggtggttca ggtacagcgg cggtttgcac cggaagccgt
     3481 cagccattca ctggcactgg cggcgtcact ggacgcgcag gggtacagcg tcagtgacat
     3541 catccatatc ctgatggcgg aaggaggtca ggcatgagcg cacgttcaca ggcactggtt
     3601 cctctcagca cagagcaaca ggccgcatgg cgggcggtgg cggagaccga aaaacgccgc
     3661 catcagggta acacgctggc tgaatatccg tatgcggggg cgtttttccg ctgtctgaac
     3721 ggaagccgca ggatatcgct gtctgacctg cgatttttca tgccgtccct gaccgcagaa
     3781 gaactgcacg gcaaccgtct tcagtggctg tatgccatcg atgtgctgat tgaaacacag
     3841 ggcgaggtct gcctccttcc ccttcccggt gatgcagcag agcgcttgtt cccgtcggtg
     3901 cggtttcgtg tcagggaacg cagtcgccat aaatccgcgc tggtcatgca gaagtacagc
     3961 cgccagcagg cgagggaggc agagcagaaa gcccgtgcgt accaggcgct ggtcgctcag
     4021 gctgaaatcg aactggcatt ccattcgccg gaaacagtcg gaagctggca tgcacgctgg
     4081 agtgaccggg tggcagagca tgacctggaa accctgttct ggcagtgggg cgagcgtttt
     4141 ccgtcactgg ccggaatgga acgctggcag tggcaggata tgccattctg gcaggtcatc
     4201 gcagaggctg gtatggcggc aagggtggtc ggtcatgctg ttcgtgagat ggagcggtgg
     4261 atggtgccga acaaactccg ggaggcagcg tgatgcggca acaatcacac ggatttcccg
     4321 tgaacggtct gaatgagcgg attattttca gggaaagtga gtgtggtcag cgtgcaggta
     4381 tatgggctat gatgtgcccg gcgcttgagg ctttctgcct catgacgtga aggtggtttg
     4441 ttgccgtgtt gtgtggcaga aagaagatag ccccgtagta agttaatttt cattaaccac
     4501 cacgaggcat ccctatgtct agtccacatc aggatagcct cttaccgcgc tttgcgcaag
     4561 gagaagaagg ccatgaaact accacgaagt tcccttgtct ggtgtgtgtt gatcgtgtgt
     4621 ctcacactgt tgatattcac ttatctgaca cgaaaatcgc tgtgcgagat tcgttacaga
     4681 gacggacaca gggaggtggc ggctttcatg gcttacgaat ccggtaagta gcaacctgga
     4741 ggcgggcgca ggcccgcctt ttcaggactg atgctggtct gactactgaa gcgcctttat
     4801 aaaggggctg ctggttcgcc ggtagcccct ttctccttgc tgatgttgta cgggcatgaa
     4861 caacctgact tcaggaaggt cgtttccctt caggaacggg gatgaacgcg cgcctgcggc
     4921 gcccgttctt ttcccccgcc ttctctggtt atggcctgtc agaaatcaca tcaccttccc
     4981 tgtgattatt ctctttttct tcctgctctg attctgacta ctgcagtgtt gtcctgtctg
     5041 ccgggcgcac cttcggctcc cggctgcctg gcggcatccg gtcgtcaggc ccgactccga
     5101 caggcagctg ctggcgcacc tgccagtctc cgccgaaccc gctggcgcgg tttcgggctg
     5161 tcgctacggt ccgatgcgtg aaaaccccgg gcttgcagcg accgttccgg tcgcgccgcc
     5221 tctgtcgcgc cgtggcctgc gctcctggca tcccggcaaa agaccgccgg gatgtaaggg
     5281 gcaacgccgt ttcgcaaacc gcgcttcacg tcgttgcggc gggtccctgc ccgttgttct
     5341 gtccggtgcc attcacctgt ttaagcccct gtcgccgggg gcaaagacag ccacaaccgt
     5401 ctccgccagt ccaggggtga aatgtacatc ccgcaaaact ttttgcccag aagcaggcaa
     5461 aaacttttgc ggtctgcccc cggacagtcg tttccgtccg tggcagtgat gccccttgcg
     5521 gcgaaggttc tcaaaaaggt gatggtgaat accggaggga acaaaaggca ggtccccgca
     5581 aaacgtcaaa accgcggctc ccgaaactca acagatgatg ggggctgaaa accagagaga
     5641 ggagaaagca ggatgagtac ccgaaatatc cacgttaaca ccgcgtcgta cacgcttctt
     5701 gttgccggga agaaaaaaaa caccggggaa gagtgggacg tcctggaatt cagcagcctg
     5761 actgagctga aaaaatatcg caaaagccac ccggaaaaga tggccttcag ttatagctac
     5821 gcgctcagtc agggtgtgga taagcagttc cgccatatca acattgcgga agccgatcac
     5881 ttcaaacagt tcctgcgtca gattaagcgt gccggcctcg atatccgggc gatctgctga
     5941 ccggatgtga tgaatacccc tcctgattgt tgcaatcttg ttaatggaga tcggggggga
     6001 tgatattaag cggaaaaatt ttttattaaa tttattcttt cataaaggag tagctgttat
     6061 gcgattagct tcccgttttg gtcggtataa ttccatccgc cgtgaacgtc ctttaacgga
     6121 tgatgaatta atgcagttcg tgccttcggt attttccggt gataaacatg agtcccggag
     6181 tgaacgttat acgtatattc caacaatcaa tatcatcaat aagttacgtg atgaaggttt
     6241 ccagccattc tttgcctgtc agagtcgggt tcgtgatttg ggacgtcgcg aatacagtaa
     6301 acatatgtta cgtcttcgca gggaagggca tattaacgga caggaagttc ctgaaattat
     6361 cctgcttaat tcacatgatg gttcatccag ttatcagatg atccccggaa tttttcgttt
     6421 tgtctgcaca aatggcctgg tgtgcggaaa taattttggc gaaatccgcg ttccacataa
     6481 aggtgatatt gtcgggcagg ttatcgaggg agcgtatgaa gtgctcggtg tctttgataa
     6541 ggtcactgat aatatggagg cgatgaaaga aattcatctt aacagtgacg agcaacattt
     6601 atttggcaga gctgcactga tggtcaggta tgaagatgaa aataaaacgc cagtgacacc
     6661 tgaacagata attactcccc gtcgtcggga agacaaacag aacgatctct ggacaacctg
     6721 gcagcgggtt caggagaata tgataaaagg tggattatcg gggcgaagtg cctccgggaa
     6781 aaataccagg acaagagcca ttacaggtat tgatggtgat ataagaatca acaaggcgtt
     6841 atgggtgatt gccgaacagt tcagaaagtg gaagtcatga taatataagt aaagccccga
     6901 aaaaattttc ggggctttac ttatattatc atttttcggc attgctgtgt cgtctgatat
     6961 acctcaattt catcaaccgg ctttccggtt gtctgtaaat attgtgcctg cgaaaaatgg
     7021 tctggcagga ggctgcgcag ttaccgggta tatataagat gggttcccgc ttcgcggggc
     7081 ttcggccagt cagtattgtc actcatatga tatttttgtg tgtggccttc catgccgctt
     7141 ttgcggcata accggtatct gacaatgtct gtaagattaa ttgttctgca cgctgttaat
     7201 ttccggaagt gatttcttcg tggccggaag ccggatacct ttactgcttt ttatcccggt
     7261 ataaatccgg taaacatctg atgcgtaggc aagacgtctc tggttctggc gttcggtttt
     7321 cctgaatccg gcattgtatg caccaacggc ctcccaggtg acgccccatt ttttaaatgc
     7381 tattgccaga taataagcac cggtataaat gttcatgcag ggatctgttg tcagatgttc
     7441 tggcttaatt ccgtagcggg ccagttcgtt aaaatgctgg gaatctacct gcatcagtcc
     7501 gctgccatat cccgttaccg gattaatacc gatggcatta acccggtaac gggattcttg
     7561 ccatgatatt gctctcagta aatcaggatc tattttgtaa tcccggcctg caagatcaaa
     7621 gcaatcagtg gcttggcaga tctcatttat aaacatcagg cagatggcta acatccattt
     7681 tttcattttt ccacctctgg tgactttatc cgtaaataat ttaacccact ccacaaaaag
     7741 gctcaacagg ttggtggttc tcaccaccaa aagcaccaca cactacgcaa aaacaagttt
     7801 ttgctgattt gctacttgaa tcattaactt atgttttaaa taatgtattt taatttattt
     7861 tacattataa aaaggattca ttggtgaatc gcatatgatt caccaatgaa tttaacacaa
     7921 ctcatagatg aatcagtaat aataaaacaa cgagttaaag cgaattgact ctagattcaa
     7981 ttcgtgatgt gatttgctgc atgaatcgta attttcgtaa cttgcgtcac gattcgattc
     8041 atcgatgaat tgtctttttt ttatatgtga aataaatttg atgcgataag aaaataaaga
     8101 ggtatacata tggcgaaagt gcaggcttat gtcagtgacg aaattgtcta taaaataaat
     8161 aaaattgtgg aaagaagacg tgcagaagga gctaaaagta cagatgtaag tttctcaagc
     8221 atatccacta tgcttctcga attagggctt cgtgtatatg agactcagat ggagcgtaaa
     8281 gagtctgcat ttaatcagac tgagtttaat aaattgcttc ttgaatgcgt tgtaaaaaca
     8341 caatcatcag tagcgaaaat tttgggtatt gagtctctca gtcctcatgt ctccggaaat
     8401 ccaaagtttg aatatgcgaa tatggtcgaa gatatcaggg agaaggtgtc atctgagatg
     8461 gagaggttct ttccagaaaa tgatgagggt taaaaaagaa ttaaaagttg atttgcttca
     8521 aataacagga ttttgatctg gttcaatttg atgacaatta accgcataaa ggttatatta
     8581 attatgtggt taatgccacg ttaaaacttg atattaaaaa tcgccgatga gagaaaggcc
     8641 acttaacagt caatctgtaa ataaatacat tcttaacgtt caaaatatct acaggaattc
     8701 tcctgttcct gtttgtgtcc gtaacaaaaa aaggaaaatc ctttatgcca atggggcctt
     8761 tattgaactc ttttccaaag aagataaacc cttctctggt gaaagttatg tacgtctgca
     8821 ggttgaaatt tttctttcat cacttgagct tgaatgccag tctcttgggc atggttccgc
     8881 attttgtcgt cgttttaatt ttcatggaga aatctatcaa ataaggatgg aaaatgtttc
     8941 attttataat gaagaatctg ttgttctatg gcaaattaat atatttcctg actatccatt
     9001 ctttcgtgtg gaaaaagaaa attattatca tcgtgatagc tatgtacagt ctgttatatc
     9061 gaatatgaca gcaaaatcat tggttgtttt ttgtttctat gcattagggt ataaacatat
     9121 aaatatagct aaagaattaa aaatcacaga agtggcttct aaaaaaagga ttaaaaagat
     9181 taatgcggat atcaataaaa gattgggttc cttcgatttg ttcaggaata aatgtattaa
     9241 gacaggtgta atgtacgaaa tattaaatgt tttgagtgaa tatatgggtg ttaaaaaaat
     9301 gtaataaagc agggctcgtt ttgggcccat tttcgacagg ccatttctgt gcaatcatga
     9361 ttttattggt caggaaacag aggtgaattg agtgaggagg cgtaacgcga gaggcgggat
     9421 aagcagaaca gtctcagtgt atctcgatga agatacaaat aaccgactta ttcgggccaa
     9481 agatcgtagc ggtagaagt
//
LOCUS       DAEEUE010000133         9493 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.133, whole genome shotgun sequence.
ACCESSION   DAEEUE010000133 DAEEUE010000000
VERSION     DAEEUE010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9493
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.133"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..190)
                     /locus_tag="KLK84_003604"
     CDS             complement(<1..190)
                     /locus_tag="KLK84_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HBE2010780.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLGEKA"
     gene            710..1405
                     /gene="minC"
                     /locus_tag="KLK84_003605"
     CDS             710..1405
                     /gene="minC"
                     /locus_tag="KLK84_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBE2010781.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1429..2241
                     /gene="minD"
                     /locus_tag="KLK84_003606"
     CDS             1429..2241
                     /gene="minD"
                     /locus_tag="KLK84_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBE2010782.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2245..2511
                     /gene="minE"
                     /locus_tag="KLK84_003607"
     CDS             2245..2511
                     /gene="minE"
                     /locus_tag="KLK84_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBE2010783.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2883..3227)
                     /locus_tag="KLK84_003608"
                     /pseudo
     CDS             complement(2883..3227)
                     /locus_tag="KLK84_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3262..3381)
                     /locus_tag="KLK84_003609"
     CDS             complement(3262..3381)
                     /locus_tag="KLK84_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBE2010784.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3342..3527)
                     /locus_tag="KLK84_003610"
     CDS             complement(3342..3527)
                     /locus_tag="KLK84_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010785.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3628..3801
                     /locus_tag="KLK84_003611"
     CDS             3628..3801
                     /locus_tag="KLK84_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010786.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3803..4147
                     /locus_tag="KLK84_003612"
     CDS             3803..4147
                     /locus_tag="KLK84_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HBE2010787.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4151..4486
                     /locus_tag="KLK84_003613"
     CDS             4151..4486
                     /locus_tag="KLK84_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HBE2010788.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4543..6864)
                     /locus_tag="KLK84_003614"
     CDS             complement(4543..6864)
                     /locus_tag="KLK84_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBE2010789.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVYNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6981..7190)
                     /locus_tag="KLK84_003615"
     CDS             complement(6981..7190)
                     /locus_tag="KLK84_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010790.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7941..9464)
                     /locus_tag="KLK84_003616"
     CDS             complement(7941..9464)
                     /locus_tag="KLK84_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBE2010791.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVMVE"
BASE COUNT         2659 a         2203 c         2080 g         2551 t
ORIGIN      
        1 cggctttttc gccgagatat ttccttgtta actctgggga aatgccatct gagttcacac
       61 aaatatacgg gtcgcaaaca acgccgcacg tcggagattg aagtccgcca gcgtcatgtc
      121 cagacgccat tgatgagaac atcatcccca cacctgacaa tccgatataa ataatacgca
      181 tcatattcat agcacatcct gataatccaa ccgtacaaca cttatgctca tttcgtcatc
      241 aatccgtaat caaaaccgct gacctggacg agttacgatg acctgacaga agtatcagac
      301 gtcttctgcg gtttctatga tagagcaatt ctgtggcgga tattaagata acgggtatct
      361 gttctttaat gatgctgcaa aaaatggaaa gttaagaaag cctggcgtgg tgaaaaaata
      421 cacttgcctc aatactggat atttaaacag gtctggagat gctgtcacga ttagggtgcg
      481 gaaaaaggaa gcgtaaataa gaagaatcaa aagtacgacg gcaatgggtt gattgacaag
      541 ggtatttttt aagctatgaa tcagcgccat ttatcacaga atagactttt actctgaata
      601 aatgggaggg tgacttgcct caatataatc cagactataa catgccttat agtcttcgga
      661 acatcatcgc gcgctggcga tgattaatag ctaattgagt aaggccagga tgtcaaacac
      721 tccaatcgag cttaaaggca gtagcttcac tttatctgtg gttcatctgc atgaggcaga
      781 acctaaggtt atccatcagg cgctgaaaga caaaatcgct caggccccca catttttaaa
      841 acatgccccc gttgtactca acgtcagtgc actggaagac ccggtaaact ggtcagcgat
      901 gcataaggcg gtttcggcaa ccggtttgcg ggttattggc gtaagtggct gcaaagatgc
      961 gcaacttaaa gccgaaattg aaaaaatggg gctgcctatc ctgacggaag gaaaagaaaa
     1021 agcgccacgt ccagctcccg caccgcaggc tccagcgcaa aatacaatgc cggtcacaaa
     1081 aacgcgttta atagataccc cggtgcgttc cggtcagcgt atttatgctc cacaatgtga
     1141 tctgattgtt acaagccacg ttagcgctgg ggccgaattg attgccgatg ggaacattca
     1201 tgtctatggc atgatgcgcg gtcgtgcgct ggcaggggca agtggtgacc gggaaacgca
     1261 aatattttgt acgaacctga tggcggaatt ggtgtccatc gcaggtgaat actggctgag
     1321 tgatcaaatc ccagcagaat tttatggcaa agcggcgcga ctgcagttag tcgaaaacgc
     1381 tttgaccgtt caaccgttaa attgatccct ttttaacaag gaatttctat ggcacgcatt
     1441 attgttgtta cttcgggtaa agggggtgtt ggtaagacaa cctccagcgc ggccatcgcc
     1501 actggtttgg cccagaaggg aaagaaaact gtcgtgatag attttgatat cggcctgcgt
     1561 aatctcgacc tgattatggg ttgcgaacgc cgggtcgttt acgatttcgt caacgtcatt
     1621 cagggcgatg caacgctaaa tcaggcgtta attaaagata agcgtactga aaatctctat
     1681 attcttccgg catcgcaaac acgcgataaa gatgccctca cccgtgaagg ggtcgccaaa
     1741 gttcttgatg atctgaaagc gatggatttt gaatttatcg tttgtgactc cccggcaggg
     1801 attgaaaccg gtgcgttaat ggcactctat tttgcagacg aagccattat taccaccaac
     1861 ccggaagtct cctcagtacg cgactctgac cgtattttag gcattctggc gtcaaaatca
     1921 cgccgcgcag aaaatggcga agagcctatt aaagagcacc tgctgttaac gcgctataac
     1981 ccaggccgcg taagcagagg tgacatgctg agcatggaag atgtgctgga gatcctgcgc
     2041 atcaaactcg tcggcgtgat cccagaggat caatcagtat tgcgcgcctc taaccagggt
     2101 gaaccggtca ttctcgacat taacgccgat gcgggtaaag cctacgcaga taccgtagaa
     2161 cgtctgttgg gagaagaacg tcctttccgc ttcattgaag aagagaagaa aggcttcctc
     2221 aaacgcttgt tcggaggata agttatggca ttactcgatt tctttctctc gcggaagaag
     2281 aacacagcca acattgcaaa agaacggctg cagattattg ttgctgaacg ccgtcgcagc
     2341 gatgcagaac cgcattatct gccgcagttg cgtaaagata ttcttgaggt catttgtaaa
     2401 tacgtacaaa ttgatcctga gatggtaacc gtacagcttg agcaaaaaga tggcgatatt
     2461 tctattcttg agctgaacgt gaccttaccg gaagcagaag agctgaaata agcccgctgt
     2521 aaaaacgcat ttatcttcaa ggcagagtca tttctgcctt gagtttttca tccctctcat
     2581 ccacgttgtg gtaaagcggc gagtattctt gctgatactc ctcattgcta tttccacctc
     2641 cccctttaca ccttaaggct gttcccacgc acagaacaaa atgtttatgc ttggttgagt
     2701 aaataaccat gttattagtg tgtatatact gggagatgat aaccccgcaa tttcattatt
     2761 cattgttctc ccataggatg aggtttcccg ccgtaactgg cgggcttttt ttgccccaga
     2821 attttctctt tcaacatcct gtaaacgaaa actgcgccga agcgcagttt aatcgttgat
     2881 ggtcagaacg accagttcac accagccacc gcgttccacg gggactccac accggcacca
     2941 tggctatacc ccaccccgaa atgcccgctt aacgtactgc tgaatgaggc tttaatacct
     3001 gcctggtata ttccacgtct gcccgacaaa tcattgacaa aattaccgtc actattcact
     3061 ttcacccgat tatcatcgac aaattctttg cgcacagccg ccttcaggca gggttcaatt
     3121 tccataccgt tccccagacg catgttgtaa ctcagcgttg cacccagttc acgatatata
     3181 ctgcgggtat cgactgattt cgattccatg ccattggata aatgatagta gcgtctaagt
     3241 gacctaagct ctgctgcaac atcagcccgc aggcaccaga ccaggggatt catcctgaag
     3301 agacagcgca ggtgtattgt gttcaccgct caccaaagac atcatgatga aatgatgata
     3361 tcccgcataa gaaagaggca ttttttacac gcagtgcgct gaagtgtagt tggataaaaa
     3421 atcactcttt tcagataata ctgtcgtacg cttatcttta gacaatgagg ccgtcggaaa
     3481 aataaaatga ctaatgtaat cgttgctgat gaatatcttc tgctcacata aaaatcacac
     3541 aataatattg ataaatcaga ttatcttacc tttacaacat tgcgttaaca gtcaatgaca
     3601 cttcttgaat aaaaatattg tcaatcaatg agttattcct catttaagat tattctcatt
     3661 cacgtaaaaa acataattcc tatcatcaca gccacgctca ttttgaatta tctgaataat
     3721 agcgagcgtt ctttagttaa gcagatctta attgaagacg aaattattgt gtgtgcaact
     3781 tacttaatac cggacatcta agttgaagaa aaaaatactt gccttcgggc ttatctcagc
     3841 attgttctgc tccacgccag cgatggcgga tatgaaccgc accacgaaag gtgcattgct
     3901 aggcgcaggc gttggtttac ttactggcaa tggtgttaat ggcgtactga aaggtgcagc
     3961 tgttggcgct ggtgttggtg cagtaacaga aaaaggccgc gacggtaaaa atgcgcgtaa
     4021 aggtgcaaaa gtgggtgccg ccgttggtgc tgtcactggc gttctgaccg gcaatggcct
     4081 cgaaggggcg attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc tgggtaaaat
     4141 gaaataagga atgatgatga aaaaatttgc tttactggcg ggcctgtttg ttttcgcacc
     4201 aatgacctgg gcgcaggact acaatattaa aaacggtttg ccgtcagaaa cctatattac
     4261 ttgcgcagaa gccaacgaaa tggcaaaaac tgacagcgca caggttgcag aaattgttgc
     4321 ggtaatgggt aatgccagcg ttgccagccg tgatttaaaa attgagcaat ctccggaact
     4381 gagcgccaaa gtggtagaaa aactgaatca ggtttgcgct aaagacccgc aaatgctgtt
     4441 gatcacggca attgatgaca ccatgcgtgc tatcggcaaa aaataatatt tcatatgtac
     4501 cgcgtcgatg aatacgacgc ggttttttag ctaacggcgg gatcagaact tatatttcag
     4561 gcccaccatc actgcggtgt cattgtagcc attatcaccc agctgcacgc ccacattccc
     4621 ccagacgctg gccgctggat tcaggttgcc atttacgcca agtttaattt cccccaaatt
     4681 actcacccca tcttgttcga ctttcacacc gttcattgag acagcaaaat ctttactgtt
     4741 atggatccag ttcatttcaa caaaaggttc aaagtcgtca ccgtgcggac cgctgttttt
     4801 ctcctgagta cgaataaagg tgcggaagcc gagtcgggtc tggatgttgt tatttgcgtc
     4861 attctcaata cgtgagccat tggcttccgt gtgcgcatta tgatcgacgc ccatccagat
     4921 agcctgtgcc tgtggctgga agatccacgt atatttcgcc gcttccagac caaaggattt
     4981 attcagggca aaaccgtaac ccgcttccag cgagacggtt gcacctttcg cgctatagga
     5041 ttcataggct aattcatccc ctttcacgga gtttttaaac cagctatatt gcgcccagga
     5101 gtcaatgtat gcgccttttt tactgatgtc atcggcaaac caagtggcat acagccctgc
     5161 gctatagcct ctgacactcc ctttcgaacg ataatccgac acgctggaat gagttaagtt
     5221 gtagtcgcgg gcataaccag ccatcactcc caaacgccaa ctgtcactat cggtaaaacc
     5281 accggttaac agatcgcccc ctaactgcga gacgtagcga tgcgaggtcg ttctcaactg
     5341 tccgttgctg tcacgccagg cattatgccc gccaatttga cgtagccaaa gtcggcttga
     5401 acgctcctgt tcagttacag gatcgatgta gcgcgtttca cccgcacggt cgttcagacg
     5461 cataacaaaa agagtgttag cggctgccag gttggcgacg taacttccag cttcggggcg
     5521 caggacgggg gtaggcgcaa cagtaggcgc tgaacttcct ccggagtttg tttcaccgcc
     5581 gctcgttgcc tggaagttag tcagatacca gtttttattg gtcgcttcgg tattacctcg
     5641 ggttaatgaa tattcgtagg caccggcgaa aatcctcgaa tccttaataa attccccatt
     5701 tgattccccc tcaacgctga taatctctat cccatttaac gtgtaagctc cgctgcctcc
     5761 tgcattggta actttaactc gggttgttcc agaggtattt ccattaataa cgactttatc
     5821 cgtagctgaa ttatcatcat ccaattcagt tctgagatgc aatacgccat tattaccaac
     5881 gtaattttca gttattgtta atcttgttgg ttcgacatcc cgtccatctg cccgggaaat
     5941 atagaccgta ctattataaa ccgtgaggtt gctcaccgtt gagtcagctt taatatccca
     6001 ggtactatta tctgaaagcg acagataggt atgagtgtta tcatctgttg aaatattagc
     6061 tgaacccgtt atttttgaat tagttgcatc gacggtcaaa tgacctttaa tattattgat
     6121 aaaaacatct gcagccgatg atacctcact atccgtaatg gtcacgttac tcgtctcatt
     6181 tttatggtag aggatttcat ttgctgaccg tagtgagcta ccagataaaa tatctaccgt
     6241 tgcttcctga ccatttatgg aagactccag cacaattcca gacccctgat gcgcgactgc
     6301 agatgtattt ttcagcgtca ccgtgccgcc attataaatt ttagcaatcg cggcgtcagg
     6361 acccgttgta taaatttctc cctggttaaa tgaaactgtc ccctggcggg cagcgatggc
     6421 atgagcattg tccccatgcg tggtgatatc aacatcggtt aatgtaacgc tggcattggt
     6481 ataatcaagc gtgtcaccag ccttgacgac agtgccatta gcgtacgcgg cataactttc
     6541 tgttccgtgg gttgtgatct ccccttttgc catatccagt tttgctatgg cattattttc
     6601 attacttcct gtatcggcag tactttcttc atcaagagaa gatacacacg cacacgcgct
     6661 tccgggagat gcacacgcaa gaacgccgac tgcgtgatta cccgcagtaa caataaatgt
     6721 atcatctccg tcagaaaaag agggggtaac cgaaccgtca gagttgactc ttattatggt
     6781 gtcagtattc aggtttccac tcgtttgact tagcaaacca attgagtaat cccccgttgt
     6841 gacaatcgtg tttttaccct gcaaaataac agcaccgcca aattcactta cgattgcgct
     6901 agctgttaat gctgggctta gaggactatt gtcagaagga tataaaccgc ccgttgttac
     6961 aatgacgcca ttgttaatgg ttaacttact accctctcct gcaacggata cccgtaactt
     7021 ccattacccg ttgttataac tagcgcatta tcaatatctt ttacttttcc atcttttatt
     7081 gccacagcat gtttatattc atcgtgctga ataaactgtt cagcatatgc attacccacc
     7141 gccagagcaa taagtcccaa actaaacccg gggagttttt tcagtttcat agattaacta
     7201 tccattcatt ggattaacaa acattcctgg tcaaataacc tatgagctca gattaccaaa
     7261 acggagggta aagacaaaaa caaaagcaca aaaaatttaa atagataaac agcgacaata
     7321 tataaatgat aaataagtcg gcattatcat ttttcacctt attttcatga cattgatcac
     7381 tttggggtga ctattttgtt taacaaataa ctcttcgaca tcaaaattat taatagttgc
     7441 catggtaatt atttcggtga tatataaaaa caactgtgat aacaccaagg ttgacaacta
     7501 tttgcgcaat aattcctttc cttcaatgat ccaggcgttc actgccagca acccattcag
     7561 ccgcatactg acagtgagaa actgaaagat tattgctgat ccctgtggct aacgagtctt
     7621 tctcttaaat cgattaacca gattgatcgc cagccaaaca agggcattaa gcaaatttat
     7681 cgtcacaagc acttctccca gcccggtact gggttttgca ataaggatga ccaggagggt
     7741 gatcagcata aatgcaatgg aaaagatgat atagagagtg aaataatcca gattattact
     7801 attattcatg ctactaatgg caatcctgtc aaaactttca aacaattata gcaaacaaaa
     7861 taatgaacaa tagtaacaag aggcaacgag ctaattagct gtctataatt acaccgcact
     7921 atggccttac tgatcaattt tcactcaacc ataaccttca ccttcggttt agaaaggata
     7981 attttgacca ggtcgatgta tgtaacaggt ttatagaaat aataaccctg ctgaaatgtg
     8041 atattatttt ggttcagata gccaagttgt tctttcgttt cgacaccttc agcgacgata
     8101 ctgatcgaaa gtttacgcgc cagatccaat acacaatcca gaattcgggt tgattctggg
     8161 tctgcgttta cgcggccaac gaaactgtga tcaattttga tataatcaat atgcaggtca
     8221 tgaagataag aaagccctga gtagccagta ccgaaatcat ccagcgcgat gacaaaacca
     8281 ttttcatgca gaatgttcaa ccgctgaacc agactttcat caacattcaa tggctcacgc
     8341 tcggtgactt ccagaacaag gtttaaatcg cggcgggtaa aactgtcacg gaaatttaaa
     8401 cactcgtcga caaacgtcgg cgaaataata tgcgaagcgc taaaattaat tccaatatga
     8461 aaaccttccg gcagtttact cgcgatagcg ttcatctgtc tggcaacctg attcatcagg
     8521 ctttgtgtaa gcggaacgat taatccggat ttttcagcaa gtggaataaa tgccgcgggt
     8581 gaaatatatc caccgtgagg ttgtttccag cgggctaaca cctcaactcc ccgcaatgtc
     8641 ccttcccgac catttaccac aggttggtaa aaagggacga tctccccatt atttattgcc
     8701 cgtcgcagga tctcttcagg cgttgcgctt ttattgaaat atctatcaag caggaaggca
     8761 gcggcgcagg cgattaacag aataaatatt aaaatcccaa agcctttatc gataagtctg
     8821 tagaagctaa agagtggcgg cgggttaaag ataatagtga aatgatattt tgtggagtga
     8881 accttctcgg tatagggata atgtccatta agcgtcatca catcacccgt tggtccaata
     8941 atgtcatccg ccacgcgtaa tacatacctt acccctttca agggtacatt aagcgccccg
     9001 cgaatatgtt gatcacttat cgtaaccaaa atgcgcgtat ctgcaaattg gctctgatag
     9061 agcaggatag gtaatctatt aacggtgtcg attgccggag ccaacagtaa attactgtcc
     9121 gggacaacag gaatacgaga cagcaggacc cgattcccag gcagggatgt acaccagact
     9181 attccctgtt tgagaatgat gattgtgcgc aggtgaggtt tcagagctgc ttcagtgcca
     9241 agctgatatt gctcctcgag gtcgcattcc ttcccggcaa tgttaaccgc tgtttgagtc
     9301 gcctgtgacg cttcttcaag tatatgattg atattgttgg cggcatatct tgctcccgcc
     9361 aggtactctg cttttgcgga ataccagagc tggatgttca aaatagcgac accggtaata
     9421 aacaagcata tcgcaacgag gatgggtatg agtgtattgc gcatttgctt gtcccggcca
     9481 aagggtttaa tac
//
LOCUS       DAEEUE010000134         9153 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.134, whole genome shotgun sequence.
ACCESSION   DAEEUE010000134 DAEEUE010000000
VERSION     DAEEUE010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9153
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.134"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..923
                     /locus_tag="KLK84_003617"
     CDS             <1..923
                     /locus_tag="KLK84_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE2010792.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIP
                     FLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVA
                     FISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTT
                     HSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1175..2092
                     /gene="oxyR"
                     /locus_tag="KLK84_003618"
     CDS             1175..2092
                     /gene="oxyR"
                     /locus_tag="KLK84_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HBE2010793.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWENRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="KLK84_003619"
     CDS             complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="KLK84_003619"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HBE2010794.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3796..4446
                     /gene="fabR"
                     /locus_tag="KLK84_003620"
     CDS             3796..4446
                     /gene="fabR"
                     /locus_tag="KLK84_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HBE2010795.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4446..4805
                     /locus_tag="KLK84_003621"
     CDS             4446..4805
                     /locus_tag="KLK84_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HBE2010796.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4845..5933)
                     /gene="trmA"
                     /locus_tag="KLK84_003622"
     CDS             complement(4845..5933)
                     /gene="trmA"
                     /locus_tag="KLK84_003622"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HBE2010797.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRAQNLNVHLIGRATKTKIALD
                     QDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELYCGNG
                     NFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNGVREF
                     NRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLETLSQ
                     THKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6047..6237
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6302..8146
                     /gene="btuB"
                     /locus_tag="KLK84_003623"
     CDS             6302..8146
                     /gene="btuB"
                     /locus_tag="KLK84_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HBE2010798.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8091..8948
                     /gene="murI"
                     /locus_tag="KLK84_003624"
     CDS             8091..8948
                     /gene="murI"
                     /locus_tag="KLK84_003624"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBE2010799.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2094 a         2217 c         2469 g         2373 t
ORIGIN      
        1 tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg
       61 cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg
      121 ctatcgcacc tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg
      181 ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc
      241 gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgtcg gttaaaccgc
      301 gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga
      361 tgattttagg tttctctggc gtcaattata ccggttggct ttacattgcg tggttacccg
      421 gttatttgca agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc
      481 ctttcctcgc ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga
      541 aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt
      601 cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta
      661 tctcaatggc gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg
      721 tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg
      781 tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta
      841 atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta
      901 ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga
      961 ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt
     1021 tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct
     1081 gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat
     1141 cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct
     1201 ggtggcattg gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca
     1261 gccgacgctt agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga
     1321 gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg
     1381 taccgtgctg cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat
     1441 gtccggaccg ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat
     1501 tatcccgatg ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac
     1561 ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt
     1621 gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat
     1681 ctatgaagat cacccgtggg agaaccgcga atgcgtactg atggccgatc tggcagggga
     1741 aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt
     1801 tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa
     1861 catggtggcg gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg
     1921 caaacgcgat ggggttgttt atctgccatg cattaagccg gaaccacgcc gcactattgg
     1981 cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat
     2041 ccgcgcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta
     2101 acgcagctac ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt
     2161 actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt
     2221 cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca
     2281 aaattttcag cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact
     2341 gggcgcggcc cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca
     2401 cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg
     2461 tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg
     2521 ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat
     2581 acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg
     2641 ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt
     2701 ttttacccga cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact
     2761 cttcgttgtg acgaatcact acgccactgt tccagaagtg gtaggagaga gaatctgaca
     2821 tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat
     2881 ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta
     2941 catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg
     3001 tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag
     3061 cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa
     3121 agcgagcgtt cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc
     3181 gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga
     3241 gcagtcggga atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg
     3301 cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt
     3361 gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt
     3421 cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag
     3481 ggcttacctg ttcttataca taaaagcaac agaatggtaa cattctatcg cgggtaagcc
     3541 aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt
     3601 acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg
     3661 tttccgctgt acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc
     3721 acaatactgg agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc
     3781 aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg
     3841 ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg
     3901 cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac
     3961 gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg
     4021 cgccaggcgc gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca
     4081 tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc
     4141 ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa
     4201 cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc
     4261 gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa
     4321 caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct
     4381 tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac
     4441 gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggtt
     4501 tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc
     4561 cgattatttc cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca
     4621 tgtcggtagg cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca
     4681 gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct
     4741 ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg
     4801 agtaatcggt atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt
     4861 aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg
     4921 accttgtgcg tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag
     4981 atatacagaa tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg
     5041 cgcggtgggt cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg
     5101 cggttaaact cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata
     5161 atttgtacgt tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt
     5221 ttggcgattt cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta
     5281 ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc
     5341 cattccagca tctgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga
     5401 cgatagatca tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc
     5461 gcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt
     5521 gcttccgctt cctgacgcca ctcatcatcc agctttttat ggtacagcag ggaaaccacc
     5581 gcctgattac tcagcgtggt gaggtaatca atctggaaca acttgtggcg cagaacggga
     5641 ttattacgca cacccgcaat catcgccgtc atcaactggt tgatgagttc actggcggcg
     5701 gggaagctat ccacgcggat gcggcttttg gtttgttgat cgaaaatgat gtgatacagg
     5761 tcatcgccat cgtgccagat gcggaactcc gcgcgcatcc ggtaatgact gaccggcgag
     5821 cgaaacactt ccggaaccag gtcagaaaac ggtgccatca tactttgcaa acgtaccact
     5881 ttttcggcta actgcgcttc atactgttct gttggaaggt gttcgggggt catgatgtat
     5941 cctgaaaaat taaagtacgc ggcgattgta gggattgctc atcagatgtc cagatcttga
     6001 tgaattccta tttgtgagct acgtctggac agtaacttgt tacaacctgt agcatccact
     6061 tgccggtcct gtgagttaat agggaatcca gtgcgaatct agagctgacg cgcagcggta
     6121 aggaaaggtg cgatgattgc gttatgcgga cactgccatc cggtgggaag tcatcatctc
     6181 ttagtatctt agatacccct ccaagcccga agacctgccg gccaacgtcg catctggttc
     6241 tcatcatcgc gtaatattga tgaaacctgc ggcatccttc ttctattgtg gatgctttac
     6301 aatgattaaa aaagcttcgc tgctgacggc gtgttccgtc acggcatttt ccgcttgggc
     6361 acaggatacc agcccggata ctctcgtcgt tactgctatc cgttttgaac agccgcgcag
     6421 cactgtgctt gcaccaacca ccgttgtgac ccgtcaggat atcgaccgct ggcagtcgac
     6481 ctcggtcaat gatgtgctgc gccgtcttcc gggcgtcgat atcacccaaa acggcggttc
     6541 aggtcagctc tcatctattt ttattcgcgg tacaaatgcc agtcatgtgc tggtgttaat
     6601 tgatggcgtc cgcctgaatc tggcgggggt gagtggttct gccgacctta gccagttccc
     6661 tattgcgctt gtccagcgta ttgaatatat ccgtggaccg cgctccgctg tttatggttc
     6721 cgatgcaata ggcggggtgg tgaatatcat cacgacgcgc gatgaacccg gaacggaaat
     6781 ttcagcaggg tggggaagca atagttatca gaactatgat gtctctacgc agcaacaact
     6841 gggggataaa acacgggtaa cgctgttggg cgattatgcc catactcatg gttatgatgt
     6901 tgttgcctat ggtaataccg gaacgcaagc gcagacagat aacgatggtt ttttaagtaa
     6961 aacgctttat ggggcgctgg agcataactt tactgatgcc tggagcggct ttgttcgcgg
     7021 ctatggctat gataaccgta ccaattatga cgcgtattat tctcccggtt caccgttgct
     7081 cgatacccgt aaactctata gccaaagttg ggacgccggg ctgcgctata acggcgaact
     7141 gattaaatca caactcatta ccagctatag ccatagcaaa gattacaact acgatcccca
     7201 ttatggtcgt tatgattcgt cggcgacgct cgatgagatg aagcaataca ccgtccagtg
     7261 ggcaaacaat gtcatcgttg gtcacggtag tattggtgcg ggtgtcgact ggcagaaaca
     7321 gactacgacg ccgggtacag gttatgttga ggatggatat gatcaacgta ataccggcat
     7381 ctatctgacc gggctgcaac aagtcggcga ttttaccttt gaaggcgcag cacgcagcga
     7441 cgataactca cagtttggtc gtcatggaac ctggcaaacc agcgccggtt gggaattcat
     7501 cgaaggttat cgcttcattg cttcctacgg gacatcttat aaggcaccaa atctggggca
     7561 actgtatggc ttctacggaa atccgaatct ggacccggag aaaagcaaac agtgggaagg
     7621 cgcgtttgaa ggcttaaccg ctggggtgaa ctggcgtatt tccggatatc gtaacgatgt
     7681 cagtgacttg atcgattatg atgatcacac cctgaaatat tacaacgaag ggaaagcgcg
     7741 gattaagggc gtcgaggcga cagccaattt tgataccgga ccactgacgc atactgtgag
     7801 ttatgattat gtcgatgcgc gcaatgcgat taccgacacg ccgttgttac gccgtgctaa
     7861 acagcaggtg aaataccagc tcgactggca gttgtatgac ttcgactggg gtattactta
     7921 tcagtattta ggcactcgct atgataagga ttactcatct tatccttatc aaaccgttaa
     7981 aatgggcggt gtgagcttgt gggatcttgc ggttgcgtat ccggtcacct ctcacctgac
     8041 agttcgtggt aaaatagcca acctgttcga caaagattat gagacagtct atggctacca
     8101 aactgcagga cgggaataca ccttgtctgg cagctacacc ttctgaacca cgtcccaccg
     8161 tgctggtgtt tgactccggc gtcggtgggt tgtcggtcta tgacgagatc cggcatctct
     8221 taccggatct ccattacatt tatgctttcg ataacgtcgc tttcccgtat ggcgaaaaaa
     8281 gcgaagcgtt tattgttgag cgagtggtgg caattgtcac cgcggtgcaa gaacgttatc
     8341 cccttgcgct ggctgtggtc gcttgcaaca ccgccagtac cgtttcactt cctgcattac
     8401 gcgaaaagtt cgacttcccg gttgttggtg tcgtgccggc gattaaacct gctgcacgtc
     8461 tgacggcaaa tggcattgtc ggattactgg caacccgcgg aacagttaaa cgttcttata
     8521 ctcatgagct gatcgcgcgt ttcgctaatg aatgccagat agaaatgctg ggctcggcag
     8581 agatggttga gttggctgaa gcgaagctgc atggcgaaga tgtttctctg gatgcactaa
     8641 aacgtatcct acgcccgtgg ttaagaatga aagagccgcc agataccgtt gtattgggtt
     8701 gcacccattt ccctctacta caagaagaac tgttacaagt gctgccagag ggaacccggc
     8761 tggtggattc tggcgcagcg attgctcgcc gaacggcctg gttgttagaa catgaagccc
     8821 cggatgcaaa atctgccgat gcgaatattg ccttttgtat ggcaatgacg ccagaagctg
     8881 aacaattatt gcccgtttta cagcgttacg gcttcgaaac gctcgaaaaa ctggcagttt
     8941 taggctgatt tggttgaatg ttgcgcggtc ggaaaattat tttaaatttc ctcttgtcag
     9001 gccggaataa ctccctataa tgcgccacca ctgacacgga acaacggcaa acacgccgac
     9061 gggtcagcgg ggttctcctg agaattccga cagagaaagc aaaaataaat gcttgactct
     9121 gtagcgggaa agcgtattat gcacaccccg cgc
//
LOCUS       DAEEUE010000135         9019 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.135, whole genome shotgun sequence.
ACCESSION   DAEEUE010000135 DAEEUE010000000
VERSION     DAEEUE010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9019
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.135"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            146..2330
                     /gene="espL4"
                     /locus_tag="KLK84_003625"
                     /pseudo
     CDS             146..2330
                     /gene="espL4"
                     /locus_tag="KLK84_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(2299..4036)
                     /gene="aceK"
                     /locus_tag="KLK84_003626"
                     /pseudo
     CDS             complement(2299..4036)
                     /gene="aceK"
                     /locus_tag="KLK84_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(4220..5523)
                     /gene="aceA"
                     /locus_tag="KLK84_003627"
                     /pseudo
     CDS             complement(4220..5523)
                     /gene="aceA"
                     /locus_tag="KLK84_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
     gene            complement(5553..7154)
                     /gene="aceB"
                     /locus_tag="KLK84_003628"
                     /pseudo
     CDS             complement(5553..7154)
                     /gene="aceB"
                     /locus_tag="KLK84_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(7423..8352)
                     /gene="metA"
                     /locus_tag="KLK84_003629"
     CDS             complement(7423..8352)
                     /gene="metA"
                     /locus_tag="KLK84_003629"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBE2010800.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            8509..8952
                     /locus_tag="KLK84_003630"
     CDS             8509..8952
                     /locus_tag="KLK84_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBE2010801.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2360 a         2180 c         2173 g         2306 t
ORIGIN      
        1 cgcaagatct cattccaccg aaacttttac ggctgcatta tgagattgag cgatttatga
       61 tattttttta gttgtaatca tattaaattt tttcactgtt ctgatagtta aaattcaaga
      121 catcaataaa caatgagata tttaaatgat tactcgtatt cctcgtagtt ctttctctgc
      181 aaatattaat aatacagccc agacaaatga acaccaaacc ctgagtgaat tgttttataa
      241 agaactcgag gataaatttt ctggcaagga gctggcgacg cctctattaa aaagcttctc
      301 agagaactgt agacaaaatg ttcgtcatat ctttagcaac aaggattttg tcattaaatt
      361 ttccacgtcc gtcttacaag ctgataagaa agaaattacg ataattaata aaaacgaaaa
      421 cacgacactc actcaaacca ttgctacaat atttgaagaa tacctaatgg aaattttacc
      481 tcaacgctca gacactcttg ataaacaaga attaaaccta aaatcagata gaaaagaaaa
      541 agaattccca agaattaaac ttaatggtca atgttatttc ccggggcgcc ccaaaaccgt
      601 atagtatacc gacacattgc tgcacaatat attaatgata tttatcagaa tgttgattac
      661 aaaccccatc aagatgatta ctcttcagct gaaaaatttc tcactcactt caacaaaaaa
      721 tgcaaaaacc agactttggc gttgatttcc agccgtcctg aggggcgttg cgttgctgcc
      781 tgcggtgatt tcgggctagt tatgaaagca tattttgaca agatggaatc aaatggcatc
      841 agtgttatgg cagccatatt actggtggat aaccatgctt tgacggtccg gctaagaata
      901 aagaacacaa ctgaaggatg tacccattac gtggtttcgg tttatgatcc taatgtaact
      961 aacgataaaa taagaattat gagcgaaagc aaagaggata ttaaacacta ttctctgatg
     1021 gattttatga atgtagatta tagcctcctg aaatggtcaa atgatcatgt tattaaccaa
     1081 tctgttgcaa taattccagc acttccgaaa gaacagctat tgatgttaaa aggaactgtg
     1141 gatgaaataa cccctccatt atcaccagca acgatgaatt tgctaatggc aattggtcag
     1201 aatcaccaac tcacgcaact gatgattcag ctccagaaaa tgccagaact acatagaaca
     1261 gaaatgttga ctgcctataa tagtattaac ctccccggtt tatatttggc tataaattat
     1321 ggcaatgcgg atatcgttga gactattttc aattcattgt cagaaacagg atatgaaggg
     1381 ttactctcga aaaaaaatct catgcatatt ctggaggcaa aagataaaaa tggtttttct
     1441 ggattatttt tagcgatatc acgtaaggat aaaaatgttg taacctcgat tctgaacgtc
     1501 ttacctaaac tggccacaac acatcattta gataatgaac aggtgtataa attcctgagt
     1561 gccaaaaata gaacgtccag ccatgtttta taccatgtta tggcgaatgg tgatgccgac
     1621 atgctgaaaa tttttttggt cgcgttacct ttgttaattc gcacatgtca tttgactaaa
     1681 gaacaggtac tcgatctcct gaaggcaaag gatttttatg gttgcccaag actatacctg
     1741 gcgatgcaaa atggacatag cgatatcgtg aaagttattc tcgaagcatt gccctgtctg
     1801 gcccaggaaa ttaacatttc agcttccgat attgtagatc ttctgaccgc taaaagtctt
     1861 gcgcgcgaca cgggtttgtt tatggccatg cagcgcggac atatgaacgt tattaatact
     1921 atttttaacg cattacccac tctttttaat acgtttaaat tcgataaaaa aatatgaagc
     1981 ccctcctcct ggcaaataat tctaatgaat atcccggttt gttttcagcg atacagcata
     2041 aacaacaaaa cgttgtagag acggtttatc ttgctttatc tgaccatgca cgcctgtttg
     2101 gatttaccgc tgaagatatt atggattttt ggcaacacaa agcgccacaa aaatactctg
     2161 cctttgagtt ggcttttgaa tttggtcacc gggttattgc tgaattaatc cttaatacat
     2221 taaataagat ggctgaaagc tttggcttta cggataaccc tcgatacatt gcggagaaaa
     2281 attatatgga agctttactc aaaaaagcat ctccccatac cgtacgctaa atctttgcct
     2341 gcgccgatac gcataaacat cttccacatg cccttcacgt atgcggtttt gtagtgcgcg
     2401 ccagtaatca gcgcggaaca ggtcggcgtg catctcttca aacagcggac caatacgcgg
     2461 gtcggcgcat agccagtggc gaaactcttc cgggaaaaca tcgcccggcg agacgctgta
     2521 ccacggttcg ctggcaagtt cgtcttccgg atagcgcggc ggcgggatgt cgcggaaatt
     2581 cacttccgtc atgtagcaaa tttcatcgta atcataaaaa accacacgcc cgtgacgggt
     2641 gacaccgaag tttttaaaca gcatgtcgcc agggaaaatg ttagcagcgg caagctggcg
     2701 aatagcgtta ccgtattctt caatggcgtc gcgcaactgc tgaccttcca cttgttccag
     2761 ccagatattg agcggcacca tccgccgctc gattatagag atggcgaatt acaatctgct
     2821 caccaagatc ggtgattttt tccgctgctt cctggagcag taattccatt aataccgggg
     2881 aaatatgccg cttctccagc acaaagtttt caaactcctg ggtgtccgcc attcggccca
     2941 cacggtcgtg ctctttcacc agttgatagc aggcacgaac gtgagcggca gacatctctt
     3001 tctgcggcgc gaacttgtct ttgatgactt tgaatacccg atcaaagccc ggcagcgtaa
     3061 acaccaacat caccattcca cgaatacccg gcgcttcaat gaactgctca ttacagccct
     3121 gtagataaac gagatattcg cggtagcttt cggttttggc gtgcttctgg cagccgatag
     3181 ccatatacaa ttcagcagtg gttttacctg gcagaatttc ccgtagccac tcgaccagcg
     3241 ctgcgggcag cggtgcataa accataaaat aagaacgcgc aaagccaaaa acaatgctcg
     3301 cttcggcggt cgtcgtcagg caggtatcaa taaataactc gccgtcgtcc gtctggtgga
     3361 tcggcagtaa gaagggcaac gtgccggaag gcgtgatcag tttgcccacc agccaggcgg
     3421 ctttattgcg gtaaaacagt tcgttcgcca cctgtaaatg actttccgcg aggttgtctg
     3481 tccccagcgt ttccgtcaga tggcgaatga tgtaatgaat gtcacggctt ttattctgcc
     3541 agcgcaggcg cagcggcagg tcgctgataa cgcgcatcag tagagattcc cagccgtgat
     3601 cggggtgaaa gtctttcgcc agcgggcggg gaatggtacg aaagcggcgc tctggctgag
     3661 agctaaagat aaaaagccgc tcgggagtaa gcgagcggtg gtcaaataac cgacagtaca
     3721 cggagttaaa aaagctctcc gcaatctcga agcgcgggta atccggcaac agccgcgtgt
     3781 aatgctcttt aacgcgcagt aaaaatgccg cgtccgtact ttggccgtta gtaatgcagc
     3841 gcagttgctc cacgaccaga ccaacgtgat gatcgtaaag atggatacgg tttttcatcg
     3901 cctgctggac agcatgccag tcggcctgtt cgaaacgctg ctgcgccccg gaagtcactt
     3961 cgaggaatcg accatactga gcgtcgaagc cttgcaaaat ggtttgagca atcagtaatt
     4021 ccaggccacg cggcatttag cgccctcatc aggagcagag aattgccgga tgcggcgtaa
     4081 acgccttatc cagcctacgt tcggcaacgg ttgtaggcat gataagacgc gcaagcgtcg
     4141 catcaggcat cgtgcaccga ttgccggatg cggcgtaaat gccttatccg gcctacagtc
     4201 agcaacgttt gtaggttgct tagaactgcg attcttcagt ggagccagtc agcgcggtga
     4261 ctgaagacgt gccgccctga ataatagtcg tcactttatc gaagtaacct gtacccactt
     4321 cctgctggtg agatacgaag gtatagccat ctttcgcggc ggcaaattcc ggctgctgca
     4381 ctttctcaac atagtgcttc ataccttcgc cctgcgcata agcgtttgcc aggtcgaaca
     4441 tgttaaacca catgctgtgg atacctgcca gggtgatgaa ctggaacttg tagcccatat
     4501 ccgacagctg ctgctggaag ctggcaatag ttttgtcgtc gaggtttttc tgccagttga
     4561 acgacggcga gcagttataa gccagcagtt tgcccggata tttcgcgtgg atagcttgtg
     4621 caaagcgacg cgccagttcc agatccggcg tggaggtttc acaccagacc aggtcagcat
     4681 atggcgcata cgccaggcca cggctgatcg cttgctcaat gcccgcatga gtacggaaga
     4741 agccttcact ggtacgctcg ccggtaataa attcgctgtc atacgggtcg caatctgagg
     4801 tgatcagatc cgccgcatca gcatcggtac gtgcaaccag cagggttgga acgcccgtca
     4861 cgtcagctgc cagacgcgcc gcgaccagtt tctgaatagc ttcctgagtt ggcactaaaa
     4921 ctttgccgcc catgtgaccg catttcttca ctgacgccag ctgatcttcg aagtgaactg
     4981 ccgcagcacc ggcttcaatc atcgctttca tcagttcaaa ggcattcagg acaccgccaa
     5041 aaccggcttc cgcatcggca acgatcggca ggaagtaatc gacatagcgc ggatcgcccg
     5101 gctcaatgcc cacggaccat tggatctgat cggcacgacg gaaggtgttg ttgatccgct
     5161 ccaccacagc tggcaccgag tttgccggat agagcgactg atccggatac atgctggccg
     5221 ccaggttagc gtccgccgct acctgccatc ccgacagata gactgcttca atacccgctt
     5281 tcgcctgttg cagcgcctga ccgccagtca gtgcaccgag gctgttgatg tagctttttt
     5341 cgactcaccg tgcagcagac gccacatttt cgctgcgccc agttgcgcca gcgtgcattc
     5401 aggattgact gaaccgcgta atttcaccac atcttccgca ctgtatgggc gagtaatgcc
     5461 ttcccaacgc ggttgagtcc actctttctg taattcttca atttgttgtg tacgggtttt
     5521 catgtgcaga tgctccatag ttatgtggtg gtttacgcta acaggcggta gcctggcagg
     5581 gtcaggaaat caattaactc atcggaagtg gtgatctgtt ccatcaagcg tgcggcatcg
     5641 tcaaaacggc cctgggagaa acgttcttcg cccagttcgc tggcaatgac tttcatctct
     5701 tcgcccagca tctggcggaa caaggctttg gtcaccggtt tgccattgct caacgttttt
     5761 tgatgatgga tccactgcca gatcgaggta cgggaaattt cagccgtcgc cgcatcttcc
     5821 atcaggccat aaatcggcac gcagccgttg ccggagatcc acgcttcgat gtactgcaca
     5881 gccacgcgaa tgttagcgcg cataccttct tcggtgcgtt caccatcaca aggtgccagc
     5941 agctgatcgg cagtaatcgg cgcgtcttgt tcgcgcaata cttcaagctg atttttacgg
     6001 gagccgagaa tgtcgttgaa taccgccatt gccgtgtccg caaggcctgg gtgagcgatc
     6061 catgtgccat cgtgaccgtt attggcttcc agcgctttat ccgcttttac tttgttgagc
     6121 acctggttat tacgctcttc atctttgctc ggaataaacg cggccatgcc gcccatcgca
     6181 aaagcaccgc gtttatggca ggttttaatc agcaggcgtg agtaagcatt caggaatggt
     6241 ttatccatcg tcacggcctg tctgtctggc aggacacgat cgggatagtt tttcaacgtt
     6301 ttgatatagc tgaagatgta atcccagcga ccgcagttca gaccaacaat atggtcgcgc
     6361 agcgcgtgaa ggatttcgtc catctggaac acggcgggca gcgtttcaat cagcaacgtc
     6421 gccttgatgg tgccgcgcgg cagattaaag cgatcttctg catagctgaa gacttcgctc
     6481 caccaggccg cttcctgcta ggactgggtt ttcggcagat agaaataggg accgctgccc
     6541 tttgccaaca gtgcctgata gttgtggaag aaatagagcg caaaatcaaa caggctaccg
     6601 gggattgcct caccacgcca ggtgacatgt ttttccggca agtgcagacc gcgtacccga
     6661 caaatcaaaa ccgctggatt gggcttgagc tgataaattt tgcctgcttc attggtatag
     6721 ctgatggtac cgttaaccgc atcgcgcagg ttaatttgcc cgtcgatcac tttgttccag
     6781 tctggtgcca gtgaatcttc gaaatcggcc ataaagactt tcacattggc gttcagtgcg
     6841 ttgatcacca tcttgcgctc taccgggcca gttatctcta cgcggcggtc ttctaagtcc
     6901 gcaggaatcc cgcgaatttt ccaatcagca tcgcgaatgg aagccgtttc cgaaataaaa
     6961 tcaggcaacg ttccgttatc aatatcttgc tgctgctgaa tgcgcgctgc cagaagttta
     7021 ttgcgttgtg gcgtaaaatg cgtcaccagc tcagtcagaa attctaccgc ttcggcagta
     7081 agaatttgct tctcctgctc gccatacggc cttgtgaaag ccagttcatc ggttgttgtt
     7141 gcctgttcag tcatcgtgca gctcctcgtc atggatccga aaacttcccc tctgtgaacg
     7201 aaggaacgtt gtgcgctttt cgttcagcac aactaagact actcatttaa aatgcaaaat
     7261 caaaaacaat ttccatttta attaaaaata cttgataact tattgataac aatatgaata
     7321 aaatttattc acttgctgag gtgcgtttcg gaaataaaaa aggcacccga aggtgcctga
     7381 ggtaaggtgc tgaatcgctt aacgatcgac tatcacagaa gattaatcca gcgttggatt
     7441 catgtgccgt agatcgtatg gcgtgatctg atagacgtaa tagttgagcc agttggtaaa
     7501 cagcaaatta ccgtgactac gccagctcgc tcgcggtttg ttttgcggat cgttgtgcgg
     7561 gaaatagtta tacggtacat ccgggtctag tccggcttcc acatcgcgta aatattcctg
     7621 cgccagcgtt tgcgcatcat attcgggatg gcccgtcaca aaggcaatgc gcttatcttt
     7681 gctggcaaac agatatgcat ccccttcttc cgtctctgcc agaatttcca gatcggtgta
     7741 atcacgaatc aacgctgccg gaaagtcagc atagcgcgaa tgcggtgcca ggaatgaatc
     7801 atcaaagcca cgcgtcagaa gcgcatgagg atggagaata tgatgctcgt aaacgccaga
     7861 gagtttgtcg gtgcgagttt gcttaggaat gccgtagagg atattgagcg cggcctgtac
     7921 cgcccagcag acaaacagcg tcgaggtgac gtgatctttc gaccactcca gcacctgttt
     7981 gatctgcggc cagtaagcga catcattaaa ctccaccagg cccagcggcg caccggttac
     8041 aatcagaccg tcaaagttct gttcctgaat atcttcaaag ttacagtaga agttgttcag
     8101 atgctctgcg ggcgtgttgc gcgattcacg ggaatcgatg cgcaacagct gaatatcgac
     8161 ctgcaaaggt gagtttgaaa gcaggcgcag aaactgattt tcagtttcaa tcttcttcgg
     8221 catcaggtta aggatcagaa ccttaagtgg acgaatttcc tgaccagacg cacgagaagt
     8281 tgtcatcaca aagacgtttt cttcacgcaa gaaattgacg gcgggtagct cgtccggcac
     8341 acgaatcggc ataacctgat tacctcacta catacgctta tacgtttaga catccagata
     8401 gctgaagata accagaaaat ttgccaatgt cgagcctgca tgttgaaggt gagaaagttt
     8461 caaggaaaag gctacgttag aatataagaa tgacgaaaag gagagaaaat ggttattagt
     8521 attcgccgct cacggcatga ggaaggggag gaactcgttg cgatttggtg tcgatctgtc
     8581 gatgccaccc acgatttttt atcagcagag tatcgggccg agctggaaga gctggtgcgt
     8641 tcctttctgc cggaagcacc gttgtgggtc gcggttaatg aacgggagca gccggttggg
     8701 tttatgttgt taagtgggca gcatatggat gcgctgttta tcgatcctga tgtgtgcggc
     8761 tgcggcgtgg gtcggatgct ggtggagcat gcgctctcga tggcaccgga actgacaacc
     8821 aacgttaatg agcaaaatga gcaggcggtt gggttctata agaaggtggg ttttaaggtt
     8881 acgggacgct ctgaggtgga cgatttgggg aaaccgtatc cgttgctgaa tctggcgtat
     8941 gtgggggaat aagccgcccc tgccgtgcct gcccaagctg gagaagacgc gccagcgtcg
     9001 cacccggcat ttttttcac
//
LOCUS       DAEEUE010000136         9014 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.136, whole genome shotgun sequence.
ACCESSION   DAEEUE010000136 DAEEUE010000000
VERSION     DAEEUE010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9014
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.136"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="KLK84_003631"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="KLK84_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HBE2010802.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="KLK84_003632"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="KLK84_003632"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBE2010803.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="KLK84_003633"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="KLK84_003633"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBE2010804.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="KLK84_003634"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="KLK84_003634"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBE2010805.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4449..5573
                     /gene="dprA"
                     /locus_tag="KLK84_003635"
     CDS             4449..5573
                     /gene="dprA"
                     /locus_tag="KLK84_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBE2010806.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5545..6018
                     /locus_tag="KLK84_003636"
     CDS             5545..6018
                     /locus_tag="KLK84_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HBE2010807.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6047..6589
                     /locus_tag="KLK84_003637"
     CDS             6047..6589
                     /locus_tag="KLK84_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HBE2010808.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6594..7166
                     /gene="tsaC"
                     /locus_tag="KLK84_003638"
     CDS             6594..7166
                     /gene="tsaC"
                     /locus_tag="KLK84_003638"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HBE2010809.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYDKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7171..7989
                     /gene="aroE"
                     /locus_tag="KLK84_003639"
     CDS             7171..7989
                     /gene="aroE"
                     /locus_tag="KLK84_003639"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBE2010810.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNVFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7986..8243
                     /locus_tag="KLK84_003640"
     CDS             7986..8243
                     /locus_tag="KLK84_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HBE2010811.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8219..8773)
                     /locus_tag="KLK84_003641"
     CDS             complement(8219..8773)
                     /locus_tag="KLK84_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBE2010812.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2257 a         2188 c         2247 g         2322 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 atggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacagcgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact gaccaatcgc aaagattgct aaggcttgct tatggcaggg
     4441 agataaggat ggtcgataca gaaatttggc tgcgtttaat gagcatcagc agcttgtacg
     4501 gcgatgatat ggtccgtatc gctcactggc tggcaaaaca gtcgcatatt gatgcggttg
     4561 tattgcagca aacagggctt acattgcggc aggcacaacg ctttctttca tttccacgaa
     4621 agagtatcga aagctcactt tgttggttgg agcaacccaa ccatcattta atccctgcgg
     4681 acagcgaatt ttatcctcct caacttcagg cgacgacaga ttaccccggc gcactgtttg
     4741 ttgaaggaga actgcacgcg ctgcattcat ttcagcttgc cgtagtgggg agtcgggcgc
     4801 attcatggta tggcgagcga tgggggcgat tattttgcga aactctggcg acgtgtggag
     4861 tgacaattac gagtggactg gcgcgtggaa tcgatggtgt ggtgcataaa gcggccttac
     4921 aggtaaatgg cgtcagcatt gctgtattgg ggaatggact taataccatt catccgcgtc
     4981 gccatgcccg actggctgcc agtctgtttg aacagggtgg cgctctcgtc tcggaatttc
     5041 ccctcgatgt tccacccctt gcttacaatt tcccacgaag aaatcgcatt atcagtggtc
     5101 taagtaaagg tgtactggtg gtggaagcgg ctttgcgcag tggttcgctg gtgacagcac
     5161 gttgtgcgct tgagcagggg cgtgaagttt ttgccttgcc aggtccaata gggaatccgg
     5221 gaagcgaagg gcctcactgg ttaataaaac aaggtgcgat tcttgtgacg gaaccggaag
     5281 aaattctgga aaacttgcaa tttggattgc actggttgcc agacgcccct gaaaattcat
     5341 tttattcacc agatcagcaa gacgtggcat tgccatttcc tgagctcctg gctaacgtag
     5401 gagatgaggt aacacctgtt gacgtcgtcg ctgaacgtgc cggccaacct gtgccagagg
     5461 tagttactca actactcgaa ctggagttag caggatggat cgcagctgta cccggcggct
     5521 atgtccgatt gaggagggca tgccatgttc gacgtactaa tgtatttgtt tgaaacctat
     5581 attcacactg aagctgagtt gcgtgtggat caagacaaac ttgaacagga tcttaccgac
     5641 gcaggttttg agcgagaaga tatctacaat gccctgctat ggctggaaaa acttgctgat
     5701 tatcaggaag ggttggcaga accgatgcaa ctggcctcgg atcctctctc catgcgtatt
     5761 tatacaccgg aagagtgtga acgactggat gccagctgcc gtgggtttct gcttttcctt
     5821 gagcagattc aggtgctcaa ccttgaaact cgtgaaatgg tgatagagcg agtgctcgcg
     5881 ctggataacg cagagttcga actggatgat ctgaaatggg tgatcctgat ggtgttgttc
     5941 aacatcccgg gctgcgaaaa tgcgtaccag caaatggaag aattactctt tgaagtgaat
     6001 gaaggtatgc tgcattaatc atttctttaa ttcagcataa gttgttatgg caaaatcagc
     6061 actgttcacg gtgcgtaata atgagtcctg cccaaagtgc ggggctgaac tggttattcg
     6121 atccgggaaa cacggtccgt ttcttggatg ctcacagtat ccggcgtgtg actacgtccg
     6181 tcctctgaaa tcttcagcgg atggacatat tgtcaaagtt ctggaggggc aggtttgccc
     6241 cgtatgtggc gcaaatctgg tattacgcca gggacgcttt ggtatgttta ttggttgcag
     6301 taactaccct gaatgcgaac ataccgaact tatcgataaa ccggacgaaa cagcaattac
     6361 atgcccccaa tgtcggacgg gccatctggt ccagcgccgc tcccgttatg gcaaaacatt
     6421 tcactcctgt gatcgctacc cggagtgtca atttgccatt aacttcaaac caatagctgg
     6481 agaatgccct gagtgtcatt atccgctact catcgaaaag aaaaccgcgc agggtgtaaa
     6541 acacttttgt gccagtaaac aatgtggaaa gccggtttcg gcggaataat aacgtgaata
     6601 ataacctgca aggagacgct atcgcagctg cgatagatgt cctcaatgaa aaacgtgtca
     6661 tcgcctatcc aacggaagcc gttttcggtg tcgggtgcga tcctgatagc gaaacagcag
     6721 tgatgagact gttggagtta aaacagcgtc cggttgataa ggggctgatt ttaatcgcgg
     6781 caaattacga gcagcttaaa ccctatattg atgacaccat gttgactgac gtgcagcgtg
     6841 aaaccatttt ttcctgctgg ccaggtcctg tcacctttgt ctttcccgcg cctgcgacaa
     6901 caccgcgctg gttgacgggc cgctttgatt cgcttgctgt acgagtcacc gaccatccgt
     6961 tggtggttgc tttgtgccag gcttatgata aaccgctggt ttctaccagt gccaacttga
     7021 gtggattgcc accttgtcga acagtagacg aagttcgcgc acaatttggc gcggcgttcc
     7081 cggttgtgcc tggtgaaacg ggggggcgtt taaatccttc agaaatccgc gatgccctga
     7141 cgggtgaact gtttcgacag gggtaacata atggaaacct atgctgtttt tggtaatccg
     7201 atagcccaca gcaaatcgcc attcattcat cagcaatttg ctcagcaact gaatattgaa
     7261 catccctatg ggcgcgtgtt ggcacccatc aatgatttca tcaacacact gaacgttttc
     7321 tttcgtgctg gtggtaaagg tgcgaatgtg acggtgcctt ttaaagaaga ggcttttgcc
     7381 agagcggatg agcttactga acgggcagcg ttggctggtg cagttaatac cctcaagcgg
     7441 ttagaagatg gacgcctgct gggtgacaat accgatggtg taggcttgtt aagcgatctg
     7501 gaacgtctgt cttttatccg ccctggttta cgtattctgc ttatcggcgc tggtggagca
     7561 tctcgcggcg tactactgcc acttctttcc ctggactgtg cggtgacaat cactaatcgg
     7621 acggtatccc gcgcggaaga gttggctaaa ttgtttgcgc acactggcag tattcaggcg
     7681 ttgggtatgg acgaactgga aggtcatgag tttgatctca ttattaatgc aacatccagt
     7741 ggcatcagtg gtgatattcc ggcgatcccg tcatcgctca ttcatccagg catttattgc
     7801 tatgacatgt tctatcagaa aggaaaaact ccttttctgg catggtgtga gcagcgaggc
     7861 tcaaagcgta atgctgatgg tttaggaatg ctggtggcac aggcggctca tgcctttctt
     7921 ctctggcacg gtgttctgcc tgacgtagaa ccagttataa agcaattgca ggaggaattg
     7981 tccgcgtgaa tcaggccatc cagtttccgg acagggaaga gtgggacgag aataaaaaat
     8041 gtgtatgttt tcccgctctc gtgaatggta tgcaactgac atgcgcgatc tctggcgaga
     8101 gtctggcgta tcgctttact ggagatacgc cagaacagtg gttagcgagt tttcgtcagc
     8161 atcgctggga cctggaagaa gaagcggaaa acttaattca ggaacaaagt gaagatgatc
     8221 aaggctgggt ctggttaccc tgatccagat attcgtcctt ccatttcacg taattattcg
     8281 cggaatagcg taacccagcc ttctcttcat cacttaacgg gcggatctgt ttgacggggc
     8341 taccgagata cagatatccg ctctccagcc gtttattttg tgggaccaga ctacccgcac
     8401 caatcatcac atcatcttct actattgcgc catcaagtaa aattgagccc atcccaacca
     8461 aaactcgatt gccaatggtg cagccgtgga gcatcacctt gtgaccaaca gtgacatctt
     8521 cgccaatggt taatgggttg ccatctgggt tgtacgagga tttatgagtg acatgcaaca
     8581 tactgccatc ctggatattg gtgcgtgctc cgatctgtac ataatgtaca tctccacgaa
     8641 tcacaacgag cggccagatc cccacatcat cagccagacg aacgtcacca atcacgacac
     8701 tgctatcgtc gatcattacg cgctgaccga tttgtggaaa aagatcgcgg tatgggcgta
     8761 aaacatcaga catacttacc tcagcaataa atgatttact aatgactttg ggggcattat
     8821 tggccttgtg caagtctttt agtatgcaaa aaagcaccgt tttgtgtgcg attgcagcaa
     8881 aaagggtgaa aaaacaacaa acagaaaaaa agatcaaaaa aatacttgtg caaaatattg
     8941 ggatccctat aatgcgcctc cgttgagacg acaacgtgaa acacttcaca ggatggtcgg
     9001 aacaacgaag agaa
//
LOCUS       DAEEUE010000137         8898 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.137, whole genome shotgun sequence.
ACCESSION   DAEEUE010000137 DAEEUE010000000
VERSION     DAEEUE010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8898
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.137"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            1..2887
                     /locus_tag="KLK84_003642"
                     /pseudo
     CDS             1..2887
                     /locus_tag="KLK84_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="KLK84_003643"
     CDS             complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="KLK84_003643"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HBE2010813.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            3979..5331
                     /gene="yegD"
                     /locus_tag="KLK84_003644"
                     /pseudo
     CDS             3979..5331
                     /gene="yegD"
                     /locus_tag="KLK84_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
     gene            complement(5343..7283)
                     /gene="yegI"
                     /locus_tag="KLK84_003645"
                     /pseudo
     CDS             complement(5343..7283)
                     /gene="yegI"
                     /locus_tag="KLK84_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(7280..8041)
                     /locus_tag="KLK84_003646"
     CDS             complement(7280..8041)
                     /locus_tag="KLK84_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HBE2010814.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(8038..8697)
                     /locus_tag="KLK84_003647"
     CDS             complement(8038..8697)
                     /locus_tag="KLK84_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBE2010815.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPIDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         2174 a         2357 c         2318 g         2049 t
ORIGIN      
        1 tggttgtcct gctgacgccc ggagacgatc ctctcagggc atttttgata tgggtactgt
       61 cagaatccat cggcgctctg gcactggtgc cgctgggatt gttatttaaa ccacactatc
      121 tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta gccatcacac
      181 tgacgttaag ctggctttcg atgctgtatc tgccgtggcc ttttactttc attattgtgc
      241 tgttgatgtg gagcgccgtg cgcctgccac gaatggaagc ctttttgatc ttccttacca
      301 cggtgatgat ggtgtcgctg atgatggccg cggatccctc cctgcttgct acgccgcgta
      361 cgtacctgat aagccatatg ccgtggctac cgtttttgct gatcctgctg cccgccaaca
      421 tcatgaccat ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa
      481 cccgctttcg gaacgcgatg gaatattccg ctatcggtat ggcgttagtg ggcaccgagg
      541 gacaatggct gcaatccaac aaagcactct gccagtttct cggttacagt caggaagagc
      601 tgcgcggact cacctttcag caactgacct ggccggagga tctcaataaa gatctccaac
      661 aggttgaaaa gctgataagc ggtgaaataa acacctattc aatggaaaaa cgctactaca
      721 accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc actggtgcgc cacacggatg
      781 gcacgccgct ctattttatc gctcagattg aagacattaa cgagctaaaa cgcaccgaac
      841 aggtgaatca gcaactgatg gagcgcatca cgctggctaa cgaagcgggc gggattggca
      901 tctgggagtg ggagctgaag ccgaatattt ttagctggga taagcggatg ttcgagctgt
      961 atgaaattcc tccgcatatc aaaccgaact ggcaggtgtg gtacgagtgc gtgctgccgg
     1021 aagatcgcca gcatgccgaa aaagtgattc gttgcaatca cgctcgccct ttaagctgga
     1081 atttcgcatt gccgtgaaag atggcatccg ccatatccgc gccctcgcca accgggtact
     1141 gaataaagaa ggcgaagtcg aacgcctgct cggcattaat atggatatga ccgaggttaa
     1201 acagcttaac gaggcattgt ttcaggaaaa agagcgcctg cacattacgc tggattccat
     1261 cggtgaagcc gtagtctgta ttgatatggc gatgaaaatt acctttatga atccagcggc
     1321 ggagaagatg agcggctgga cgcaggaaga agcattaggt gttccgctcc tgacggtgtt
     1381 gcatattact tttggcgaca acggaccatt aatggagaac atttacagtg ccgacacctc
     1441 acgttccgcg attgaacaag atgtggtgtt gcactgccgg aacggcggca gttacgacgt
     1501 gcattacagt attacgccgt taagtactct ggacggcagc aatataggct cggtactggt
     1561 gattcaggac gtcaccgaat cacgcaaaat gctgcgccag ctgagctaca gcgcctccca
     1621 tgatgcactg acgcatctcg ccaatcgcgc cagttttgag aagcaactgc gtatcctgct
     1681 gcaaacggta aacagtacac atcagcgaca tgccctggtg tttatcgatc ttgatcgctt
     1741 taaagcggtg aatgacagcg ccgggcatgc ggcgggtgac gctttactgc gcgaactggc
     1801 gtcgttgatg ctgagtatgc tgcgctccag cgacgtgctg gcgcgactcg gtggtgatga
     1861 atttggtcta ctgttgccag actgtaatat cgaaagtgcg cgttttatcg ctacacgcat
     1921 tatcagtgcc gtgaatgatt atcactttat atgggaaggc cgtgtgcatc gggtaggtgc
     1981 cagtgccggg attaccttga ttgatgacaa caatcatcag gcggcagaag tgatgtcgca
     2041 ggctgatatc gcctgttatg cctccaaaaa tggtggacgg ggccgggtga cggtttacga
     2101 accacagcaa gctgccgcac atagcgaacg ggcggcgatg tcgcttgatg aacagtggcg
     2161 gatgattaaa gagaatcagt tgatgatgat cgcccacggt gtcgcttcgc cgcggatccc
     2221 ggaagcgcgt aatttgtggc tgatttcact taagctctgg agttgcgaag gcgagattat
     2281 tgatgaacaa aaatttcgtc gtagcttcag cgatccggca cttagccatg ctcttgaccg
     2341 acgggtattc cacgaatttt tccagcaggc cgcaaaagcg gttgccagta aaggcataag
     2401 catcgccctc cccctttccg ttgccggttt gagtagcgcc acgctggtga atgatctgct
     2461 tgagcagctg gaaaatagcc ctctaccacc acggttatta catctgatta ttccggctga
     2521 agcgatttta gatcacgcag aaagcgtgca aaaactgcgg ctggcgggat gtcggatagt
     2581 gctcagccag gtgggccgcg atctgcaaat cttcaactcg ctgaaagcga atatggcaga
     2641 ttacctgcta cttgatggtg agttatgcgc caacgtgcag ggtaatttga tggatgagat
     2701 gctgattacg attattcagg ggcacgctca gcgactcggg atgaaaacca tcgccgggcc
     2761 agtcgtttta cccttagtga tggatacgct ttctggcatc ggcgtcgatc tgatttatgg
     2821 tgatgtgatt gccgatgccc aaccgctgga tttgctggtg aatagcagtt atttcgcgat
     2881 taactgagag agcgtttgcc cattgcctga tgcgatgcta acgcgtctta tcaggcctac
     2941 aaatcgctca ttccgcaggc aggataaggc gctcgcaccg cttccggcga ccaacgtcat
     3001 gcttcgtctg gttgccagcc ttccgtatac cagatatgca acagcgcata agaacgccag
     3061 ggcttccagc gctcggcata acggcggatt tgtgccggtg tcatccccag aaatcgctgt
     3121 ttaatcagat aatcatccgg cagaaaaaca tctttcgcct gccagccgcg caaggcgaaa
     3181 taattcgccg tccagcgccc gatacccgga aaagtttgca gcgttttcat cgcctgctcc
     3241 acatcgcccg gtattgtcat tggtaaggtg ccctccagcg ccgcatttgc cagatgaatc
     3301 agcgcctctg cccgtttcaa cggcatacct aacgctttta atgcctgcgg gtcggctgct
     3361 gccagccgct gaggcgtggg gaagcagata tactccggaa aatcatccag ccgttcgcca
     3421 taaagctgtg ccactctggc ggtcaatttt gccgccatcg ccacgctcac cagttggcct
     3481 aaaatcgccc gcacgccctg ctcaaaagca tcaacactgc cgggtaaacg caatccgggc
     3541 cgcgccgcgc ctaacttgcc caacgcaccg ttaacaatct gcgggttaca ttgcagatca
     3601 agcaggcggc tcattttcgc cagacactca gcggcaacgg gttctaaacc tgcacttaaa
     3661 tttatgtgca gagtatggcg ggctatatcc ggaatagcag tcaccacgcc gcgatattcg
     3721 cccactgcca gactacgggc ataataactg tccgcgaccg tttccacacc actcaccgca
     3781 cgggcggcga gaaatcccaa catccacgac cagtcatacg gcggctgcca gttcaggata
     3841 tacatcgcat ctccttattc atccgctttc agcataaacg ttattcagac gttacgcttt
     3901 gctttcatat tccggttgtc gcgacggcaa catttcgcta aagtcacgcc ccttcttcac
     3961 cggcatgggg attatttcgt gtttattggt tttgattacg gtacagcaaa ctgttcagtg
     4021 gcggtcatgc gtgacggtaa accgcatttg ctaaaaatgg aaaacgacag cacgctgctg
     4081 ccttcaatgc tttgcgcgcc aacgcgtgaa gcggtaagcg aatggctgta ccgccatcat
     4141 gacgttccgg cagacgacga tgaaacgcag gcgttgttac gacgggcgat tcgttataac
     4201 cgcgaagaag atatcgatgt tacagcgaaa agcgtgcagt tcggtctttc ctcactggca
     4261 cagtacattg atgatccaga agaagtgtgg tttgtgaaat caccaaaatc gttcctcggt
     4321 gccagcggct taaaaccgca gcaggtagcg ctgtttgagg atctggtctg cgcaatgatg
     4381 ttgcacattc gccaacaggc gcaggcgcag ctgccagaag cgattactca ggcggtgatt
     4441 ggtcgcccga tcaacttcca ggggctgggc ggcgatgaag caaacgccca ggcgcagggg
     4501 attctggaac gcgcggcgaa gcgtgccggg tttaaggatg tggtattcca gtacgagccg
     4561 gtagcggctg ggctggatta cgaagccacc ttgcaggaag aaaaacgggt gctggtggtg
     4621 gatatcggcg gtggtacgac tgactgttca ttgctgctga tggggccgca gtggcgttcg
     4681 cgtctcgatc gtgaagccag tctgctgggt cacagtggtt atcgtattgg cggtaacgat
     4741 ctggatatcg ccctagcgtt taaaaacctg atgccactgc tgggtatggg tggcgaaacc
     4801 gaaaaaggca tcgccctgcc gatcctgccg tggtagaatg cggttgccat caacgacgtt
     4861 cctgcacaga gtgatttcta cagtagtgcc aacggtcgtc tgcttaacga tctggtacgc
     4921 gatgcccgcg aaccggaaaa agtggccctg ttacagaaag tctggcgtca gcgtttaagc
     4981 tatcgcctgg tgcgtagcgc agaagagtgc aaaattgctc tttcaagcgt agcggaaacc
     5041 cgcgcctcac tgccgtttat cagcaatgaa ctggctacgc tgattagcca gcgagggctg
     5101 gaaagcgccc tcagccagcc actggcgcgg attctggaac aggtgcaact ggccctggat
     5161 aacgcccagg aaaaaccgga cgttatctac ctgaccggcg gtagcgcccg ttctccgctg
     5221 attaaaaaag cgctggcaga acagttgccg ggcattccga ttgcaggcgg cgatgacttt
     5281 ggctccgtca ccgccgggct ggcacgctgg gcggaagtgg tatttcgtta aaagaaaatg
     5341 gctcagcagc ggcttaaatc agcctgcgcc tgagccagtt tttctgccgc ctgacgtaac
     5401 ggttccatca acggcatggt ccgtgctgga gcatgaaggc ggaatcgctg caactctgtc
     5461 gcgccgacag tcaacgtcga ttccagccga tgtcgtttgg cggccatttt cgccattacc
     5521 gcctgtctgt ctgctggcgt taccgcttcg ttcggcctga acacaaaacg gcgttcacag
     5581 ctcgctttcc agtcgatgac cgcctgggtc agatgatcac caaacccttt aacttgctta
     5641 acgccacgac gggtaacacc tgctgctgtt tcaataccaa aagaccataa cgccgcttta
     5701 cgcgcagggc caacgccggg aatagaggca acatcaataa aaaatccctc cagaaacttc
     5761 tacttctgcc gttcccttgc agtgtcatga agtgctgcca gagcgagttt cttttcttcc
     5821 ggtaacccga gaatttcgtc cttcattttt tcgagcatcg tccgtttggc gataaaacct
     5881 tccaggccgc ctacctgctg gatctggcgg actaaatggt catagtccat tttcgcacga
     5941 ttaaatgcct cacggcgttg ctgaacttct gctttgtacg ctttgcttgt cagactgcca
     6001 ataatccaga tagccgccag cacaggaacc aaaataatat aacgcggttc tgcctgaagg
     6061 ccgcaaagca gcagcgataa tgctgacagt gcgatctcaa gcagaatgat gtattcgcgc
     6121 cgtaacaggc ctaaaggaag cggcctgcca gtcggttgga aatgatcggg taatggcagt
     6181 tgcaatgctg gcggtgctac tgacgccatc accatcgccc agacttttgc cagcacaaaa
     6241 tccccgccgg tggtaatgac ctcttcgccg agatcaataa aatagataac gccttgatta
     6301 tccagcgtac accacgggca gtcggtcaaa tgagcgggat aaacatgcat tgccgaaacg
     6361 ggacatttct ttaactgttg gcgcagagaa tcaagtgccg ctacccacgc tttagccgtc
     6421 ggacgcccgg ttgccacgcc actttccgtg aatgcctgct gaaacatggc ttcaacatca
     6481 ctcggtaaca tcgatagcgg aatcgatcgt ggcggcggtt ttaaaccacg tcgctgatta
     6541 tctgacgcgt aggcataacg gaaatgggta atatccgtct ccagcgcatt acccgcatca
     6601 gagataagcg gcacaccaga ataaggatgc cggccaccaa acaagacgtg aaaaatcagc
     6661 aacgcaaggc caaaattacc gtgattttct gtgcgttcaa agccgacaaa tgacgacaag
     6721 gtttgtagct ctggcggcgt aaaatgcgac acgccgactt cgcataaatg cagtgtgcca
     6781 ttggcgttaa tctgaaagga gtcgctatca atcaacacca ctttgctgtc gcgacctacc
     6841 ataaaactgt tctggtttac gtcacccacg acatgcccgt gctcgtgaac cgtagcaaaa
     6901 gatgaagcaa tattgcgcgc aacatagagt agaaaatccc acgcacaatg agggtaacgt
     6961 tgacgacgat gtgccgggct atagatcata tgaatcggtt ctttaccaga aacttttggc
     7021 atcataaaac cgataacttt tccgcctcgt ccaccgtgaa gcgttgcctg cggccaggcg
     7081 acataattca acaattgcgc gtcagctgtc gcagccataa aggcaagttt gtcctgtttt
     7141 aaggcgggtg gcggcgtgtg ataaatcttg gcgacgctat cgacaaactc attgatatca
     7201 taaaccgcgc cttcaccgcc tttgcccagt tcacggcctg gcgtaacgca ctcaccagta
     7261 gcagtatata aagtgggttt catggtaacc acagcgccag tgccaacgtt ttatcatcat
     7321 ccgtacgctc gtttaccgcc gggctggaca aaaactgctt taacaattca ggtaataaat
     7381 cgagttgttc ctgcgttgct gacgccagtc cattgaaaaa cggggtaaag aaaggcacat
     7441 gcggagaatt atccaacata tttaacgcca gtcgctggat accatccgta aatgctgcaa
     7501 ctttatgcac acgctcagtg ctggtgaaag tttccagtct ggaaacggca tcttcatcgg
     7561 taatgaagtg cgtcatattg gcatattcac ccaccatcgg cgtaagcggc aattgcagac
     7621 cgtgaccaaa atccaccacc acgccgccat cgccaatctg cataatcaag gtgccattag
     7681 ccgatgaaat caatcccaga aacgtgcagg caaaatcgcg aactgctaat tctttggctt
     7741 ccgcctcggc aaaaagtcgc tgacgaacgg tcagcacaat atccgtggcg aggatatcat
     7801 taagccccaa ttccccaccc tgcacttttt gcgacatata agccatcgct tcattgacgg
     7861 cgagcatcgc gccttcgccc ccctgcgaaa cgctacctgc gccatcagca aggaacatca
     7921 ccagtaatgg ctgctggtca tttagccagg caacctgcat ctggcaggca tcctggcaag
     7981 gcaagtccgc gctgatgtgc gacgttccaa cagcggatgc ataaaccaga cgccagctca
     8041 cactgacgtc cagccttttg gcgcttccag cacaacttcc gttcccggcg tggagcggga
     8101 aaccgaacgc agcgaactgg ataaccagct aaacagttca cggaattgta atccttgcag
     8161 aggcaaaggc tgacgaacgc tgatttgtgc cagagtcttc atatccgccc cctgaacacc
     8221 gatggaaaag aaagcaaacc ttttatcttc ttccccccga aacactttgt tggctgcagc
     8281 ctgccactca tcgattggtg caccatcggt aatcaggaaa atccacgggc gataatacga
     8341 aataccattt gcacgatact cacgtttccg ctcctctacc atatccaggg ctttggtaat
     8401 ggccgcaccc attggtgtat cgccctgggc aaacaggatg ggcggaaaaa aattagcggc
     8461 gctggtaaag ggttgttcca catgcaccgg gccgaacgtc acaatgccga gttccactct
     8521 ttttaaagcc agcgaatcgg caagcagctc atcgcgaaag gtaaccaatc cggtgttaag
     8581 ttcattaata ggtctgccac tcatcgagcc agaaacatcc agcagcagaa tgcaggggca
     8641 acgtggttcc gggttactgg caaaatcgct ggtggcaaac gtgatttgtt cgctcataag
     8701 aaatcctttc ttagagaatt attgaggtcc ggtatcatac cgactgcaat aatgaaataa
     8761 gagcaaacga agaaactttc agaattagtc ccggcggcgc agaaaatttt gctattcgcc
     8821 tcgcaagaac ggagagccgt cgattgacac taagggcgta gtgacataat ttcaggagtg
     8881 agggttaggg agaggttt
//
LOCUS       DAEEUE010000138         8636 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.138, whole genome shotgun sequence.
ACCESSION   DAEEUE010000138 DAEEUE010000000
VERSION     DAEEUE010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8636
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.138"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(769..1965)
                     /gene="hemY"
                     /locus_tag="KLK84_003648"
     CDS             complement(769..1965)
                     /gene="hemY"
                     /locus_tag="KLK84_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HBE2010816.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1968..3173)
                     /gene="hemX"
                     /locus_tag="KLK84_003649"
     CDS             complement(1968..3173)
                     /gene="hemX"
                     /locus_tag="KLK84_003649"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBE2010817.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQGD
                     TPAAAPQGE"
     gene            complement(3195..3935)
                     /gene="hemD"
                     /locus_tag="KLK84_003650"
     CDS             complement(3195..3935)
                     /gene="hemD"
                     /locus_tag="KLK84_003650"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HBE2010818.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3932..4873)
                     /gene="hemC"
                     /locus_tag="KLK84_003651"
     CDS             complement(3932..4873)
                     /gene="hemC"
                     /locus_tag="KLK84_003651"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBE2010819.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5260..7806
                     /gene="cyaA"
                     /locus_tag="KLK84_003652"
     CDS             5260..7806
                     /gene="cyaA"
                     /locus_tag="KLK84_003652"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HBE2010820.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7846..8166)
                     /gene="cyaY"
                     /locus_tag="KLK84_003653"
     CDS             complement(7846..8166)
                     /gene="cyaY"
                     /locus_tag="KLK84_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HBE2010821.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8214..>8636)
                     /locus_tag="KLK84_003654"
     CDS             complement(8214..>8636)
                     /locus_tag="KLK84_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HBE2010822.1"
                     /translation="QFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRS
                     FNSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPA
                     KTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1966 a         2233 c         2220 g         2217 t
ORIGIN      
        1 agttgacggg gtatctgctg cgctggccat gagaggaaga gcggcggcga cagcaacaac
       61 aagacgtttg ggtgaaaacg aaaattccat gcaaaatgct ccggtttcat gtcatcaaaa
      121 tgatgacgta attaagcatt gataattgag atccctctcc ctgacaggat gattacataa
      181 ataatagtga caaaaataaa ttatttattt atccagaaaa tgaattggaa aatcaggaga
      241 gcgttttcaa tcctacctct ggcgcagttg atatgtaagg caggtttatt atatcgcgtt
      301 gattattgat gctgttttta gttttaacgg caattaatat atgtgttatt aattgaatga
      361 attttatcat tcataataag tatgtgtagg atcaagctca ggttaaatat tcactcagga
      421 agttattact caggaagcaa agaggattac agaattatct cataacaagt gttaagggat
      481 gttatttccc gattctctgt ggcataataa acgagtagat gctcattcca tctcttatgt
      541 tcgccttagt gcctcataaa ctccggaatg acgcagagcc gtttacggtg cttatcgtcc
      601 actgacagat gtcgcttatg cctcatcaga caccatggac acaacgttga gtgaagcacc
      661 cacttgttgt catacagacc tgttttaacg cctgctccgt aataagagca ggcgtttttt
      721 tatgcatcag aaaggtcccg gaggtgcttt cctccgggtg agaaggaact actgtggcgg
      781 gttattctgc aacgttaaca tcaaaccgtc gcgacgcatc gctgcagctt cttccggctt
      841 gtgcagtctg tccagcgcgt cggcaagcca tgcgtaatcg taggcgtccg gacgttgttt
      901 cagcgccgcg cggaaggcga gcgatgcttc ctgccattca ccgtgcttca tcagtgactg
      961 acccaatgtg ctccacaaca gcgggcgatc accgacgttt ttgatttgct ggcgtagcac
     1021 tttttccagc tgttccgggt tattggtttt cagacgcggg atcggcagca gtaggcgatc
     1081 gtcatactgg cgtttcaggc cgtcgatgat aatttgctgg gcagtatcat gatcgtcaca
     1141 ttcaataaga tgttccgcca ttgccacctg caacgctacc tgatgacgcg ttttccggct
     1201 ttggtttttc caccagttac gcaaaccttc actaccgtta tcggcacgcg cctgatccat
     1261 caggccaatc catgcctgtt gttccagcat tgcacgatgt tcttcatcac caacatgggc
     1321 tttcgccatt gatgggatga tatccagcag agaactccat gcccctgtgc ggatatacgc
     1381 ctgttccgcc agacgtaata cttcaggatg gcgtggcgta acttccagca gcttatccac
     1441 gccgtggcgt gcagcatggt tttcattacg ggccagttgc agacgtacac gggtgatttc
     1501 taccggaatg gtgtcattgc cggccagctc cgctgcgcgt tccagatgtt ggttggcgcg
     1561 tgcttcatca ccacgttgtt gcgcggcttc ggcagccagt agatagttca ccaccggttg
     1621 ttcagcgtga tcggcatttt tcgccatcag cttttcaact tgctgataat caccttccgc
     1681 cagtttcagc agcgcctgtt cggtctgctt acgcgcacgg cgacgcttac gtccgacaaa
     1741 ccatccacgg gtgtgcgcgc cagtgcggaa gatccgccgc agtagccact caatggcaaa
     1801 cagcactacc atcgccagaa tcaatatgat cgccaggccc gtgacgctgg tttcgatatt
     1861 gtagttgtcg gtctggatca gcacataacc ctgatgaccg gcaatcatcg ggccaaccac
     1921 tatccccgca atcagcaaaa caaagagtaa taacactttt agcatggtta ttctccttgc
     1981 ggcgcggctg ccggagtatc accttgcggt gcaggtgcag gtgcaggtgc aggtgcaggt
     2041 gcaggtttag cttccgttgt ccccgctgcc ggttgtgcca gcaggttacg cacgcgagtc
     2101 tgcatcagtt tttccagcat cgcctggctt tgcagggttt ccggaagatc catcgagata
     2161 ttttgctggc ttaactggtc cacctcgtcg aggaacgctt tggtggtggc atcatcagta
     2221 tcgtagtaag cacgtaccca ggtggagacg ttctccagcg cctggcgata agtctcttcc
     2281 tgatgacgcg gtacagcttg tgctgcgacc agcaggcgag agcgaatatt ttcgcgcaga
     2341 tagatatcct gatttggcgc taacagcggt acggcggtgt catcacgacg gcgaatcgta
     2401 atgaagttgt ccataaagtt ctgccagctt ttttgcagat tgatacgcca ttcgctgatg
     2461 gaactggaaa gctcttcacc gtctgaatcc atcggcgaac catcgctgtc attatcggcc
     2521 agacgcaggt tatctacctg atttgaaagc tgattaagct taaggatgat gccgtcataa
     2581 tccacctgcg atactgcaga aaggctggcg atatcatcgg taattgcccg acgaacggta
     2641 atcagactcg ggtcattcat atccgccagg ctggcgtctg cacttttcag caacgctgca
     2701 gaggtcgtga cgtcctgatc gctccacagc ttccgtccgg cgagtttcac cagaaaatcg
     2761 gcctgagcca gcagccaggt tttagcatcg ctgccggaaa tggtggcgat cttttgttgg
     2821 acttcatcca actgttttgc cagcgtttct tgctgacgat tcgcctgctt aagctgcgca
     2881 gcttgttgct taataatgcc ttccagctcg gctttttggc tctcctgggc tttttgcaat
     2941 gccgtcagtt ggttagccag ggcatcgctg gtggcggtct gattgacggc ctgttgttta
     3001 ccccagccat acaaaccgac acccgccgcc agagcaatag cgatagccac cgcgctgaga
     3061 atcaatgcag tattgttctt actctttttt tctgttgcga caggttgtga cgtggtgtcc
     3121 acggcctccc tggtctcttc aaccacggcg gaggtttttt cttgttccgt cattatggct
     3181 tcctgttatg agagttattg taatgcccgt aaaagcgcat cgttgtcagc gttatcggcg
     3241 accttaatgt cttgccagcc cagttcccgg gcgagtttcg ccaaacgctc actgacgacc
     3301 aatagtcgac agtgtagtaa ccagtgctca cgataccatt gtgggatcag cgaccagagt
     3361 tgctgcaaca tttcaccgct ggtaacaacg accatcgtca cctcgcggga ttgccagcgc
     3421 atcgcttctt ctgcaccatc gtaatggatt gcgcatcgtt gataacattc acaaaaagtg
     3481 acctcagcac cgcgcaccgt cagggtatcc ccaattagct cacgaccgcc attgccacgt
     3541 aatatcagcg cacgtttgcc cgcaatattt tgtaattcag gtaattgtag caagacttcg
     3601 ctgatttccc gatcctgtgg gtagagaatc ttctgtccgc ttacggtatg tagcgccagt
     3661 gcggtggtgc gtccaatggc gaaataagta ggtagttggg gccatttacg atcttgctga
     3721 tgcagctgtg attgggcaaa agcaaccgcg tgttgcgaga gggcaaacaa cagatcgctc
     3781 tcccccagcg ctgccagttg atcagcaagt tgcggtaatt gtcgacccgg agaaaactca
     3841 atcagcggaa aatgccaggc cacctgcccc agtgtgcgca gacggctcac taactcttct
     3901 ccagcgggag acgggcgggt gaccaggata ctcatgccgg ggcctctccg ttatagactt
     3961 cagcgaggat ctcgcgcgcg ccgttattca gtagctcttc tgccagcgaa atccccattt
     4021 gttcggcatc ttgcggcgca ccgcggcgtt caccgcgaat aatctgcgaa ccgtccggcg
     4081 cgccgaccag cgcacatagc cagatttcgc catcaataag ctcggcgtag ctaccaattg
     4141 gcacctgaca tccgccttcg agacgggtat tcatggcgcg ttctgcggta acgcgcagtg
     4201 cagtttcgtg gtgattcagc gcggcaagca gctcgcgagt gcgtgtatca tcaaggcggc
     4261 attcaatacc gactgcacct tgtcctaccg ccggaagaga aatctcgggt ggtaacgcgg
     4321 cgcgaatgcg ggactccaga cctaaacgtt ttagtccggc tacggcaaga atgatggcat
     4381 cgtattcgcc gttatctagt ttgctcaggc gagtgccgac gttgccgcgc agggagcgga
     4441 taatcagatc cgggcggcgt tcagccagtt ggcactggcg acgtaaactg gacgtcccga
     4501 cgatactgcc tgctggtaac gcatccagac tgtcatagtt attggacaca aaggcatcgc
     4561 gaggatcttc acgctcgcaa atagtgacca gccccagacc ttgcgggaat tcaaccggca
     4621 catctttcat tgaatgtacg gcgatatcag cgcgattctc caggagcgcg acttccagct
     4681 cttttacaaa taagcctttt ccgcctactt tcgccagcgg cgtatcaaga atcacatcgc
     4741 cgcgcgtcac catcggtacc agttcaacga ccaggcccgg atggctcgcc atcaacttgt
     4801 ctttgacata gtgtgcctgc cagagtgcaa gtgggctttg gcgtgtggca attcttaaaa
     4861 cattgtctaa catgcttgtt accgtcatta tcatccgtgg tccatcctaa catccttgcc
     4921 agagtgatgt cagtgttgtg gtgaaacgta gacgcctgcg caaaccgtaa aatgaggtct
     4981 ggcagtggat cctgacaggc gtttcacgcc gtcgtaataa ggaatttaca gagaataaac
     5041 ggtgctacac ttgtatgtag cgcatctttc tttacggtca atcagcaagg tgttaaattg
     5101 atcacgtttt agaccatttt ttcgtcgtga aactaaaaaa accaggcgcg aaaagtggta
     5161 acggttacct ttgacatacg aaatatcccg aatgccgcgt gttaccgttg atgttggcgg
     5221 aatcacagtc atgacgggta gcaaatcagg cgatacgtct tgtacctcta tattgagact
     5281 ctgaaacaga gactggatgc cataaatcaa ttgcgtgtgg atcgcgcgct tgctgctatg
     5341 gggcctgcat tccaacaggt ctacagtcta ctgccgacat tgttgcacta tcaccatccg
     5401 ctaatgccgg gttaccttga tggtaacgtt cccaaaggca tttgccttta cacgcctgat
     5461 gaaactcaac gccactacct gaacgagctt gaactgtatc gtggaatgtc agtacaggat
     5521 ccaccgaaag gtgagcttcc aattactggt gtatacacca tgggcagcac ctcgtccgta
     5581 gggcaaagtt gttcctctga cctggatatc tgggtctgtc atcaatcctg gctcgatagc
     5641 gaagagcgcc agttactaca acgtaaatgc agcctgctgg aaagctgggc cgcctcgctg
     5701 ggtgtggaag tcagcttctt cctgattgat gaaaaccgct tccgtcataa tgaaagcggc
     5761 agcctggggg gcgaagattg tggctccacc cagcatatac tgctgcttga cgaattttat
     5821 cgtactgccg tgcgtctcgc cggtaagcgt attctgtgga atatggtgcc gtgcgacgaa
     5881 gaagagcatt acgacgacta tgtgatgacg ctttacgcgc agggcgtgct gacgccaaat
     5941 gaatggctgg atctcggtgg cttaagctcg ctttctgctg aagagtactt tggtgccagc
     6001 ctttggcagc tctacaagag tatcgattcc ccatacaaag cggtgctgaa aacactgctg
     6061 ctggaagcct attcctggga atacccgaac ccacgtctgc tggcgaaaga tatcaaacag
     6121 cgtttgcacg acggcgagat tgtatcgttt ggcctcgatc catactgcat gatgctggag
     6181 cgtgttactg aatacctgac ggcgattgaa gatttcaccc gtctggattt agtacgtcgc
     6241 tgcttctatt taaaagtgtg cgaaaaactc agccgtgaac gcgcctgtgt aggctggcgt
     6301 cgcgcagtgt tgagccagtt agtgagcgag tggggttggg acgaagctcg tctggcaatg
     6361 ctcgataacc gtgctaactg gaagattgat caggtgcgtg aggcgcacaa cgagctgctc
     6421 gacgcgatga tgcagagcta ccgtaatctg atccgctttg cgcgtcgcaa taaccttagc
     6481 gtctccgcca gtccgcagga tatcggcgta ctgacgcgta aactgtatgc cgcgtttgaa
     6541 gcattgccag gtaaagtgac gctggtaaac ccgcagattt cacccgatct ctcggaaccg
     6601 aatctgacct ttatttatgt gccgccgggc cgggctaacc gttcaggttg gtatctgtat
     6661 aaccgcgcgc caaatattga gtcgatcatc agccatcagc cgctggaata taaccgttac
     6721 ctgaataaac tggtggcgtg ggcatggttt aacggcctgc tgacctcgcg cacccgtttg
     6781 tatattaaag gtaacggcat tgtcgatttg cctaagttgc aggagatggt cgccgacgtg
     6841 tcgcaccatt tcccgctgcg cttacctgca ccgacaccga aggcgctcta cagcccgtgt
     6901 gagattcgcc atctggcgat tatcgttaac ctggaatatg acccgacagc tgcgttccgc
     6961 aatcaggtgg tgcattttga tttccgtaag ctggatgtct tcagctttgg cgagaatcaa
     7021 aattgcctgg taggtagcgt tgacctgctg taccgcaact cgtggaacga agtgcgtacg
     7081 ctgcacttca acggcgagca atcgatgatc gaagccctga aaactattct cggcaaaatg
     7141 catcaggacg ccgcaccgcc agatagcgtg gaagtcttct gttatagcca gcatctgcgc
     7201 ggcttaatcc gtactcgcgt gcagcaactg gtttctgagt gtattgaact gcgcctttcc
     7261 agcacccgcc aggaaaccgg gcgtttcaag gcgctgcgcg tttctggtca aacctggggc
     7321 ttgttcttcg aacgcctgaa tgtatcggta cagaagctgg agaacgccat cgagttttat
     7381 ggcgctattt cgcataacaa actgcacggc ttgtcagtgc aggttgaaac caatcacgtc
     7441 aaattaccgg cggtggtgga cggctttgcc agcgaaggga tcatccagtt ctttttcgaa
     7501 gaaacgcaag acgagaatgg ctttaatatc tacattctcg acgaaagcaa ccgggttgag
     7561 gtgtatcacc actgcgaagg cagcaaagag gagctggtac gtgacgtcag tcgcttctac
     7621 tcgtcatcgc acgaccgctt cacctacggc tcaagcttca tcaacttcaa cctgccgcag
     7681 ttctatcaga ttgtgaaggt tgatggtcgc gaacaggtga ttccgttccg aacaaaatcg
     7741 attggcaacc tgccgcccgc caatcaggat cacgatacgc cgctattaca gcagtatttt
     7801 tcgtgatgag cgtgccggaa agcgaggctt atccggcatg caatcttagc ggaaactgac
     7861 tgtttcaccc gcctgttgcg tcgccgcctg ttccagcaaa tcccagaagg tttcgccgct
     7921 gcgatcgcaa atccattcat cgcctttcag gtcaaaatgg tagccgccct gtttggttgc
     7981 cagccacacc tggtgcagcg gctcctggcg gttaataatg attttgctgc cgttttcaaa
     8041 ggtaatggtc agtacgccgc cgttgatttc gcagtcgata tcgctgtcgc catccctgtc
     8101 gtccagacgc tcttcaatgg tcagccacag ttgatcagcc aggcgatgaa attcactgtc
     8161 gttcattgtt gtatcctgtt tttaagtgat ggcggcagta tagcggcatg gggtcagggc
     8221 ttcaaagttt gcacctctgc tgctgcgttc cggcatgatt catccgtcac cggaataatg
     8281 atgtctctgt tttgaacttc acgtacgttc tcctctctga cacgagtttt cgccgggcag
     8341 ggggagggcg taatccttac ctctgtatcg gtccatgtat aaacattgcg atcgtcataa
     8401 gcgaagcgat cgtttttagt tagcgaaccg ttaactggaa taatttgcaa gctctttgac
     8461 cgtaattggc ggataaagta agcatgctgt ttgccgctat taaatgagcg atcaatgacc
     8521 aggtctggcg tatccagcgg gattatctca agctggtctt caccaaatgt cgataatttt
     8581 gttacataca gccgatcttt atattgcgca atattgccag taatcagatg gaattg
//
LOCUS       DAEEUE010000139         8415 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.139, whole genome shotgun sequence.
ACCESSION   DAEEUE010000139 DAEEUE010000000
VERSION     DAEEUE010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8415
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.139"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            128..1825
                     /gene="maeA"
                     /locus_tag="KLK84_003655"
     CDS             128..1825
                     /gene="maeA"
                     /locus_tag="KLK84_003655"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBE2010823.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQRLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1959..2969
                     /gene="adhP"
                     /locus_tag="KLK84_003656"
     CDS             1959..2969
                     /gene="adhP"
                     /locus_tag="KLK84_003656"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HBE2010824.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3115..3399
                     /locus_tag="KLK84_003657"
     CDS             3115..3399
                     /locus_tag="KLK84_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBE2010825.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="KLK84_003658"
     CDS             complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="KLK84_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HBE2010826.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="KLK84_003659"
     CDS             complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="KLK84_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HBE2010827.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="KLK84_003660"
     CDS             complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="KLK84_003660"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7630..7632),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBE2010828.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
BASE COUNT         2019 a         2253 c         2140 g         2003 t
ORIGIN      
        1 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
       61 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      121 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      181 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      241 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      301 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      361 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      421 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      481 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      541 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      601 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      661 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      721 aacgaacaac caacagcggc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      781 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
      841 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
      901 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
      961 agtcggcaca ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1021 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1081 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1141 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1201 gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1261 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1321 agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa
     1381 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1441 gctggtcgcc actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat
     1501 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1561 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc
     1621 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1681 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa
     1741 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1801 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     1861 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     1921 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     1981 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2041 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2101 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2161 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag
     2221 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2281 atgccggata cagcgttgat ggcgggatgg cggaagagtg catcgtggtc gccgattacg
     2341 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2401 tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2461 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca
     2521 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atggatgcag
     2581 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     2641 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2701 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2761 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     2821 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc
     2881 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     2941 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt
     3001 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3061 aacaataatg atagtgttcc cagagaagta gcgctactga gagtaaagac actaatgaaa
     3121 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3181 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3241 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
     3301 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3361 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ttataaagcg
     3421 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     3481 cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     3541 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     3601 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3661 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3721 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3781 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3841 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3901 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3961 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     4021 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4081 gcgacgaaaa tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg
     4141 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4201 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4261 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4321 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4381 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4441 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4501 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4561 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4621 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4681 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4741 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4801 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4861 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4921 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     4981 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5041 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5101 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     5161 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5221 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5281 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5341 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5401 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5461 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5521 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5581 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5641 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5701 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5761 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5821 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5881 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5941 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     6001 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6061 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     6121 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6181 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6241 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6301 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6361 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6421 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6481 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6541 cgggttaaag ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata
     6601 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6661 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6721 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6781 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6841 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6901 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     6961 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7021 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7081 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7141 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7201 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7261 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7321 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc
     7381 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7441 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7501 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7561 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7621 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7681 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7741 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7801 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7861 gtcagaacct ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc
     7921 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7981 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8041 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8101 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     8161 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8221 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8281 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8341 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8401 ataaaattca gctta
//
LOCUS       DAEEUE010000140         8396 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.140, whole genome shotgun sequence.
ACCESSION   DAEEUE010000140 DAEEUE010000000
VERSION     DAEEUE010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8396
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.140"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            4..817
                     /gene="ttdA"
                     /locus_tag="KLK84_003661"
                     /pseudo
     CDS             4..817
                     /gene="ttdA"
                     /locus_tag="KLK84_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445032.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            814..1419
                     /gene="ttdB"
                     /locus_tag="KLK84_003662"
     CDS             814..1419
                     /gene="ttdB"
                     /locus_tag="KLK84_003662"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HBE2010829.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            1467..2930
                     /gene="ttdT"
                     /locus_tag="KLK84_003663"
     CDS             1467..2930
                     /gene="ttdT"
                     /locus_tag="KLK84_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HBE2010830.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(2973..3986)
                     /gene="tsaD"
                     /locus_tag="KLK84_003664"
     CDS             complement(2973..3986)
                     /gene="tsaD"
                     /locus_tag="KLK84_003664"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBE2010831.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            4224..4439
                     /gene="rpsU"
                     /locus_tag="KLK84_003665"
     CDS             4224..4439
                     /gene="rpsU"
                     /locus_tag="KLK84_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBE2010832.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            4550..6295
                     /gene="dnaG"
                     /locus_tag="KLK84_003666"
     CDS             4550..6295
                     /gene="dnaG"
                     /locus_tag="KLK84_003666"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBE2010833.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            6490..8331
                     /gene="rpoD"
                     /locus_tag="KLK84_003667"
     CDS             6490..8331
                     /gene="rpoD"
                     /locus_tag="KLK84_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBE2010834.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         2027 a         2254 c         2253 g         1862 t
ORIGIN      
        1 gcagtgaata agttgacaga gattgtcgct aactttaccg ccatgatttc tacccgaatg
       61 cctgatgacg tggtggataa actaaaacag ctaaaggatg ccgaaacgtc gtcgatgggg
      121 aaaattatct accatacgat gttcgacaac atgcaaaaag cgattgacct gaatcgtcct
      181 gcctgtcagg acaccgggga gattatgttc ttcgttaaag tcggttcccg cttcccactg
      241 cttggcgagc tgcaaagcat actcaaacaa gccgtggaag aggcaaccgt caaagcgcca
      301 ctacgtcaca atgcggtaga aatttttgac gaagtaaaca ccggcaaaaa taccggtagc
      361 ggcgtaccgt gggtcacctg ggacatcatc cccgacaatg acgatgcgga aatcgaagtt
      421 tacatggcag gcggcggctg cacgctacct ggccgctcga aagtgttaat gccgtcagaa
      481 ggctacgaag gcgtggtgaa attcgtcttc gaaaatatct ccaccctcgc cgtaaacgcc
      541 tgtccaccgg tactggtggg cgtgggcatc gccacctcgg tggaaaccgc cgccgtactc
      601 tcgcgtaaag ccattttgcg cccgattggc agccgccatc ccaatccaaa agcggcagaa
      661 ctggagctac gcctggaaga aggactcaac cgtctgggga ttggtccaca agggctgacc
      721 ggcaacagtt cagtgatggg cgcatcgtcg cggcacgctg ctggttcatg ccgatctcac
      781 ctttgaaaat ctgtctcaca cccggagcgc gttatgaaaa agatcctgac aaccccgatc
      841 aaagctgaag atctgcaaga tattcgcgtc ggcgatgtga tctacctgac cggtacgctg
      901 gtgacctgcc gcgacgtttg tcaccgccgt ttgatcgaac tgaaacgtcc gatcccttac
      961 gatctcaacg gcaaagcgat tttccacgct ggccccatcg tgcgcaaaaa cggtgacaaa
     1021 tgggagatgg tctccgtcgg cccgacaacc agtatgcgta tggaaagttt tgaacgtgaa
     1081 tttattgagc agaccggcgt aaaactggtg gttggcaaag gtggtatggg gccgctgacc
     1141 gaagaaggct gccagaaatt caaggcgtta catgtgattt tcccagcagg ctgcgcggtg
     1201 ctggcggcaa cccaggtaga agagattgaa gaagtgcact ggacagaact cgggatgccg
     1261 gagtcactgt gggtctgccg ggtcaaagag ttcggcccgc tgattgtctc tattgatacc
     1321 cacggcaaca acctgatagc cgaaaacaaa aagctgttcg ccgaacgccg cgatcccatc
     1381 gtggaagaga tctgcgagca cgtccattac atcaaataac cctcccggag aggctcaccc
     1441 ctctcctttt tcgcaggcat aacacgatga aaccttccac tgaatggtgg cgatatcttg
     1501 cgccgctggc ggtcatcgcc attattgctc tacttccggt tcccgcaggg ctggagaacc
     1561 atacctggct ctactttgcc gtttttactg gcgtgatcgt tggactgatc ctcgaacccg
     1621 tgccgggtgc cgtggtggcg atggtgggta tctccatcat cgccatactc tctccctggc
     1681 tgctgttcag ccccgaacag ctcgcccagc caggctttaa gttcaccgcc aaatccctct
     1741 cctgggcagt ttctggtttt tctaattcgg ttatctggct cattttcgcc gcctttatgt
     1801 ttggcacagg ctatgaaaaa accgggcttg gacgccgcat tgcgctgatt ctggtgaaaa
     1861 agatggggca tcgcacgctg tttctcggct atgcggtgat gttctccgag ctgatcctgg
     1921 cacctgtaac accgtccaac tcggcgcgtg gtgcgggtat tatctatccc atcatccgta
     1981 acctgccacc gctctatcaa tcacaaccaa acgacagcag ttcgcgcagc attggctcgt
     2041 acatcatgtg gatggggatt gttgccgact gcgtgaccag cgccattttc ctgacggcga
     2101 tggcacctaa cttgctgtta attggactga tgaaaagcgc atctcacgcc acgctgagtt
     2161 ggggcgactg gttcctcggg atgttgccgc tcagcatttt actggttctg ctggttccct
     2221 ggctggctta cgtgctgtac ccgccggtac tgaagtctgg tgatcaggtg ccgcgctggg
     2281 cagagacgga actgcaggca atgggcccgc tctgttcgcg tgaaaaacgg atgctggggc
     2341 tgatggtagg cgcgctggtg ctgtggattt tcggcggtga ttatatcgat gccgcgatgg
     2401 tcggttacag cgtggtggcg ctgatgctgc ttctgcgcat tatcagttgg gacgacattg
     2461 tcagtaataa agcggcatgg aacgttttct tctggctggc ctcgcttatc accctcgcga
     2521 ccggactcaa caataccggt tttattagct ggtttggcaa actgttagca ggcagcttaa
     2581 gtggttattc gccgacgatg gtgatggtga cgttgattgt ggtgttttat ctactgcgct
     2641 actttttcgc cagcgccacg gcgtatacct gcgccctcgc accgatgatg atcgccgccg
     2701 cgctggcgat gccggaaatc ccgctgccgg tgttctgcct gatggttggc gcggcaattg
     2761 gtctggggag cattcttaca ccatacgcca ccggacccag tccgatttac tacggtagtg
     2821 gttatctgcc aacggcggat tactggcgac tgggggcgat ttttgggctg atattcctcg
     2881 tgttgctggt gattaccggc ttactgtgga tgcccgtggt gttgctttaa gtgaggacgg
     2941 tgccggaaat tgctctccgg cacccaatag ttttacgcag ccggtaactc cgccagcggc
     3001 cagcgcggac gcacgctaac accgagatcc gccgtcgcgc ctgctttaaa ccgcaccatt
     3061 ccggcatagg cgatcatcgc gccgttatca gtacaaaatt ccggacgcgc gtagaacact
     3121 tcgccgcggc gttttttcat catttcagcc agcttcgccc gtaacgtgcg gttagcactc
     3181 acgccgcccg ccatgaccag tcgcttaaag cccgtctgat ccaacgctcg cttacactta
     3241 atcatcaacg tatcgaccac cgcatcttca aaggcgcggg cgatatcagc acgcgtctgg
     3301 tcgtcggtgc cgttgtcacg aatcgtgttc gccgcaaagg ttttcagacc agaaaagctg
     3361 aaatccagcc ccggacggtc ggtcatcgga cgcgggaaaa caaagcgccc ggcagtaccc
     3421 tgagccgcca ttttcgacag taacggtccg ccaggataat ccagccccag cagcttcgcg
     3481 gttttatcaa acgcttcacc ggcggcatca tcgatagact cgccgagcag ctcgtactga
     3541 ccaatgccag tcacgctgat taactgcgta tgaccgccgg acaccagcag cgcgacaaac
     3601 ggaaattccg gcgggttatc ttccagcatc ggcgctaaca gatgcccttc catatggtgt
     3661 accgggattg ccggaacgtt ccaggcaaac gccagagaac gccccacggt tgcgccaacc
     3721 agcagtgcgc cgactaatcc agggcctgcg gtataggcca cagcatcaat gtcttttgcc
     3781 gttaaaccag actcctttag cgccgcctgg atcaacggta cggttttacg cacatgatcg
     3841 cgggaggcca gttcaggcac gacgccgccg tagtcagcgt gcaatttcac ctgactatac
     3901 aattggttgg ctaacaaacc tttttcatcg tcgtaaatgg cgatgccggt ttcatcgcag
     3961 gaagtttcaa tacccagtac acgcatgact tgttttacct cgctttatta ccgcgcagtg
     4021 taggaccaat gcgggttgat gtaaaacttt gttcgcccct ggagaaagcc tcgtgtatac
     4081 tcctcaccct tataaaagtc cctttcaaaa aaggccgcgg tgctttacaa agcagcagca
     4141 attgcagtaa aattccgcac cattttgaaa taagctggcg ttgatgccag cggcaaaccg
     4201 aattaatcaa aggtgagagg cacatgccgg taattaaagt acgtgaaaac gagccgttcg
     4261 acgtagctct gcgtcgcttc aagcgttcct gcgaaaaagc aggtgttctg gcggaagttc
     4321 gtcgtcgtga gttctatgaa aaaccgacta ccgaacgtaa gcgcgctaaa gcttctgcag
     4381 tgaaacgtca cgcgaagaaa ctggctcgcg aaaacgcacg ccgcactcgt ctgtactaat
     4441 tccccgagag cgttgctctc cgatcagacc gagttgtagt tgtaaggccg tgcttccgaa
     4501 aggaatgcgc ggcttatttt cgtttatgaa ttgctaaaaa tcggggccta tggctggacg
     4561 aatcccacgc gtattcatta atgatctgct ggcacgcact gacatcgtcg atctgatcga
     4621 tgcccgtgtg aagctgaaaa agcagggcaa gaatttccac gcgtgttgtc cattccacaa
     4681 cgagaaaacc ccgtcattca ccgttaacgg tgagaaacag ttttaccact gctttggatg
     4741 tggcgcgcac ggcaacgcga tcgacttcct gatgaactac gacaagctcg agttcgtcga
     4801 aacggtcgaa gagctggcag caatgcacaa tcttgaagtg ccatttgaag caggcagcgg
     4861 ccccagccag atcgagcgcc atcagaggca aacgctttat cagttgatgg acggtctgaa
     4921 tacgttttac caacaatctt tacaacaacc tgttgccacg tctgcgcgcc agtatctgga
     4981 aaaacgcgga ttaagccacg aggttatcgc tcgctttgcg attggttttg cgccccccgg
     5041 ctgggacaac gtcctgaagc ggtttggcgg caatccagaa aatcgccagt cattgattga
     5101 tgcggggatg ttggtcacta acgatcaggg acgcagttac gatcgtttcc gcgaacgggt
     5161 gatgttcccc attcgcgata aacgcggtcg ggtgattggt tttggcgggc gcgtgctggg
     5221 caacgatacc cccaaatacc tgaactcgcc ggaaacagac attttccata aaggccgcca
     5281 gctttacggt ctttatgaag cgcagcagga taacgctgaa cccaatcgtc tgcttgtggt
     5341 cgaaggctat atggacgtgg tggcgctggc gcaatacggc attaattacg ccgttgcgtc
     5401 gttaggtacg tcaaccaccg ccgatcacat acaactgttg ttccgcgcga ccaacaatgt
     5461 catttgctgt tatgacggcg accgtgcagg ccgcgatgcc gcatggcgag cgctggaaac
     5521 ggcgctgcct tacatgacag acggccgtca gctacgcttt atgtttttgc cggatggcga
     5581 agaccctgac acgctggtac gaaaagaagg taaagaagcg tttgaagcgc ggatggagca
     5641 ggcgatgcca ctctccgcat ttctgtttaa cagtctgatg ccgcaagttg atctgagtac
     5701 ccctgacggg cgcgcacgtt tgagtacgct ggcactgcca ttgatatcgc aagtgccggg
     5761 cgaaacgctg cgaatatatc ttcgtcagga attaggcaac aaattaggca tacttgatga
     5821 cagccagctt gaacgattaa tgccaaaagc ggcagagagc ggcgtttctc gccctgttcc
     5881 gcagctaaaa cgcacgacca tgcgtatact tatagggttg ctggtgcaaa atccagaatt
     5941 agcgacgttg gtcccgccgc ttgagaatct ggatgaaaat aagctccctg gacttggctt
     6001 attcagagaa ctggtcaaca cttgtctctc ccagccaggt ctgaccaccg ggcaactttt
     6061 agagcactat cgtggtacaa ataatgctgc cacccttgaa aaactgtcga tgtgggacga
     6121 tatagcagat aagaatattg ctgagcaaac cttcaccgac tcactcaacc atatgtttga
     6181 ttcgctgctt gaactgcgcc aggaagagtt aatcgctcgt gagcgcacgc atggtttaag
     6241 caacgaagaa cgcctggagc tctggacatt aaaccaggag ctggcgaaaa agtgatttaa
     6301 cggcttaagt gccgaagagc gatcgggaag cccccgacag ccgcactgag aggcagcggc
     6361 aaatatataa gtacgccctc gtaattatcg ttggcggtaa acaaccgttg gatttcagcg
     6421 ttaacggctg aaggacatcg ggtcaatcgc ccaacaccaa cctcatgaaa taagtgtgga
     6481 taccgtctta tggagcaaaa cccgcagtca cagctgaaac ttcttgtcac ccgtggtaag
     6541 gagcaaggct atctgaccta tgccgaggtc aatgaccatc tgccggaaga tatcgtcgat
     6601 tcagatcaga tcgaagacat catccaaatg atcaacgaca tgggcattca ggtgatggaa
     6661 gaagcaccgg atgccgatga tctgatgctg gctgaaaaca ccgcggacga agatgctgcc
     6721 gaagccgccg cgcaggtgct ttccagcgtg gaatctgaaa tcgggcgcac gactgacccg
     6781 gtacgcatgt acatgcgtga aatgggcacc gttgaactgt tgacccgcga aggcgaaatt
     6841 gacatcgcta agcgtattga agacgggatc aaccaggttc aatgctccgt tgctgaatat
     6901 ccggaagcga tcacctatct gctggaacag tacgatcgtg ttgaagcaga agaagcgcgt
     6961 ctgtccgatc tgatcaccgg ctttgttgac ccgaacgcag aagaagatct ggcacctacc
     7021 gccactcacg tcggttctga gctttcccag gaagatctgg acgatgacga agatgaagac
     7081 gaagaagatg gcgatgacga cagcgccgat gatgacaaca gcatcgaccc ggaactggct
     7141 cgcgaaaaat ttgcggaact acgcgctcag tacgttgtaa cgcgtgacac catcaaagcg
     7201 aaaggtcgca gtcacgctgc cgctcaggaa gagatcctga aactgtctga agtattcaaa
     7261 cagttccgcc tggtgccgaa gcagtttgac tacctggtca acagcatgcg cgtcatgatg
     7321 gaccgcgttc gtacgcaaga acgtctgatc atgaagctct gcgttgagca gtgcaaaatg
     7381 ccgaagaaaa acttcattac cctgtttacc ggcaacgaaa ccagcgatac ctggttcaac
     7441 gcggcaattg cgatgaacaa gccgtggtcg gaaaaactgc acgatgtctc tgaagaagtg
     7501 catcgcgccc tgcaaaaact gcagcagatt gaagaagaaa ccggcctgac catcgagcag
     7561 gttaaagata tcaaccgtcg tatgtccatc ggtgaagcga aagcccgccg tgcgaagaaa
     7621 gagatggttg aagcgaactt acgtctggtt atttctatcg ccaagaaata caccaaccgt
     7681 ggcttgcagt tccttgacct gattcaggaa ggtaacatcg gcctgatgaa agcggttgat
     7741 aaattcgaat accgccgtgg ttacaagttc tccacctacg caacctggtg gatccgtcag
     7801 gcgatcaccc gttctatcgc ggatcaggcg cgcaccatcc gtattccggt gcatatgatt
     7861 gagactatca acaaactcaa ccgtatttct cgccagatgc tgcaagagat gggccgtgaa
     7921 ccgacgccgg aagaactggc tgaacgtatg ctgatgccgg aagacaagat ccgcaaagtg
     7981 ctgaagatcg ccaaagagcc aatctccatg gaaacgccga tcggtgatga tgaagattcg
     8041 catctggggg atttcatcga ggataccacc ctcgagctgc cgctggattc tgcgaccacc
     8101 gaaagcctgc gtgcggcaac gcacgacgtg ctggctggcc tgaccgcgcg tgaagcaaaa
     8161 gttctgcgta tgcgtttcgg tatcgatatg aacaccgacc acacgctgga agaagtgggt
     8221 aaacagttcg acgttacccg cgaacgtatc cgtcagatcg aagcgaaggc gctgcgcaaa
     8281 ctgcgtcacc cgagccgttc tgaagtgttg cgtagcttcc tggacgatta atcggtaggc
     8341 cggatcaggc gttacgccgc acccggcact agtctctctg cacaaacgcc accttt
//
LOCUS       DAEEUE010000141         8358 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.141, whole genome shotgun sequence.
ACCESSION   DAEEUE010000141 DAEEUE010000000
VERSION     DAEEUE010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8358
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.141"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            68..316
                     /locus_tag="KLK84_003668"
     CDS             68..316
                     /locus_tag="KLK84_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HBE2010835.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            381..749
                     /locus_tag="KLK84_003669"
     CDS             381..749
                     /locus_tag="KLK84_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBE2010836.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            843..1496
                     /gene="nfsB"
                     /locus_tag="KLK84_003670"
     CDS             843..1496
                     /gene="nfsB"
                     /locus_tag="KLK84_003670"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HBE2010837.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            1604..2851
                     /gene="ybdG"
                     /locus_tag="KLK84_003671"
     CDS             1604..2851
                     /gene="ybdG"
                     /locus_tag="KLK84_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HBE2010838.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIRRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(2919..4295)
                     /gene="pheP"
                     /locus_tag="KLK84_003672"
     CDS             complement(2919..4295)
                     /gene="pheP"
                     /locus_tag="KLK84_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HBE2010839.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(4396..7539)
                     /gene="cusA"
                     /locus_tag="KLK84_003673"
     CDS             complement(4396..7539)
                     /gene="cusA"
                     /locus_tag="KLK84_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HBE2010840.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(7551..8069)
                     /gene="cusB"
                     /locus_tag="KLK84_003674"
                     /pseudo
     CDS             complement(7551..8069)
                     /gene="cusB"
                     /locus_tag="KLK84_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2043 a         2326 c         2032 g         1957 t
ORIGIN      
        1 aagccgggaa ttgccttaca gaagaatatc cctgacaatg gtgttttaac ttacatttaa
       61 caatgttatg aaacaccctt tagaaacctt gaccaccgca gcaggcattt tgctgatggc
      121 tttcctctct tgcctgctgc tgcccgcccc cgcactgggg ctgacgctgg cacaaaaact
      181 ggtgaccatg ttccatctga tggatcttag ccagctttac actttattgt tttgtctgtg
      241 gtttttagtg ctgggcgcta ttgagtattt tgttctgcgc tttatctggc gacgctggtt
      301 ctcgctggcg gattaaacgt cgattatcgc cgaacttcgc ggcatacttt gcttattctc
      361 cttcgcctaa aggaacgttt atggataagc aatcactgca cgaaacggcg aaacgcctag
      421 ctcttgagtt accctttgtc gagctttgct ggccttttgg cccggagttc gatgttttta
      481 aaattggcgg caagattttt atgctgtcgt cggagctacg cggcgtcccc tttatcaatc
      541 tgaagtccga tccacaaaaa tccctgttaa atcagcaaat atacccaagc attaagccag
      601 ggtatcacat gaataaaaag cactggattt cggtgtattc cggcgaggaa atctccgaag
      661 cgttacttcg cgatctgatc aacgattcgt ggaatctggt ggttgatggt ctggctaaac
      721 gcgatcaaaa aagagtgcgt ccaggctaaa gcgggaatct atagcgcatt tttctcgctt
      781 accatttctc gttgaacctt gtaatctgct ggcacgcaaa attacttaca catggagtct
      841 ttatggatat catttctgtc gccttaaagc gtcattccac taaggcattt gatgccagca
      901 aaaaacttac cccggaacag gccgagcaga tcaaaacgct actgcaatac agcccatcca
      961 gcaccaactc ccagccgtgg cattttattg ttgccagcac ggaagaaggt aaagcgcgtg
     1021 ttgccaaatc cgctgccggt aattatgtgt tcaacaaacg taaaatactt gatgcctcgc
     1081 acgtcgtggt gttctgtgca aaaaccgcga tggacgatgc ctggctgaag ctggttgttg
     1141 accaggaaga tgccgatggc cgctttgcca cgccggaagc gaaagccgcg aacgataaag
     1201 gtcgcaagtt cttcgccgat atgcaccgta aagatctgca tgatgatgca gagtggatgg
     1261 caaaacaggt ttatctcaac gtcggtaact tcctgctcgg cgtggcggcg ctgggtctgg
     1321 acgcggtgcc catcgaaggt tttgacgccg caatcctcga tgcagaattt ggtctgaaag
     1381 agaaaggcta caccagtctg gtggttgttc cggtaggtca tcacagcgtt gaagatttta
     1441 acgctacgct gccgaaatct cgtctgccgc aaaacaccac cttaaccgaa gtgtaattct
     1501 ctcttgccgg gcatctgccc ggctatttcc cctcagattc tcctgatttg cataaccctg
     1561 tttcagccgt catcataggc tgctgttgta taaaggagac gttatgcagg atttaatatc
     1621 ccaggttgaa gatttagcgg gtattgagat cgatcacacc acctcgatgg tgatgatttt
     1681 cggtattatt tttctgaccg ccgtcgtggt gcatattatt ttgcattggg tggtactgcg
     1741 gaccttcgaa aaacgtgcca tcgccagttc acggctttgg ttgcaaatca ttacccagaa
     1801 taaactcttc caccgtttag cttttaccct gcaggggatt atcgtcaata ttcaggcggt
     1861 attctggctg caaaaaggca ccgaagcggc agatattctg actacctgcg cgcagttgtg
     1921 gatcatgatg tatgcgctgc tttcagtctt ctcgttgctg gatgttattt tgaatctggc
     1981 gcagaaattc ccggcggcat ctcagttacc gctgaaaggg atatttcagg ggattaaact
     2041 gatcggcgca atcctggtcg gcattttgat gatctcgctg ctgattggtc agtcgccagc
     2101 gattctgatc agcggtcttg gtgcaatggc tgccgtgctg atgttggtat ttaaagatcc
     2161 gattcttggt ctggtggcag gtattcagct ttccgcgaac gatatgctga aactgggcga
     2221 ctggctggaa atgccgaaat acggcgcgga tggcgcggtg atcgatattg ggttaaccac
     2281 cgtcaaagtg cgtaactggg acaataccat taccactatt cccacctggt ctctggtttc
     2341 tgactccttt aaaaactgga gcgggatgtc agcatctggc gggcgacgta ttaggcgcag
     2401 tatcagtatt gatgtcacca gtattcgttt tcttgatgaa gacgaaatgc aacgtctgaa
     2461 taaagcgcat ttgttaaagc cttatttaac cagccgccat caggaaatta atgagtggaa
     2521 tcgccagcaa ggttctacgg agtcggtatt aaacctgcgc cgaatgacca atattggaac
     2581 ctttcgtgcc tatctgaacg aatatctgcg taaccatccg cggattcgta aagatatgac
     2641 cttaatggta cgccaactgg ctccaggtga taacggttta ccgctcgaga tctacgcgtt
     2701 tactaacacc gtggtgtggc tggaatatga aagcattcag gctgatatat tcgatcacat
     2761 atttgcgatt gtcgaagagt ttggtctgcg acttcatcag tcgccaaccg gcaatgatat
     2821 tcgctctctg gcgggtgcat ttaagcagta attaaaaaaa ccgctctcat cgaatggatg
     2881 agagcggaca tcgttttgtc ggatgtagcg tgaatgcctt atttccgacg cagcgtttta
     2941 aatgccacaa acaggaatac aatccacacc ggcagcagga tcgctgacaa gcgcatatca
     3001 tccatcgtgc acatcagcag caaaatcatg ccgaggaagg cgatgcaaag atagttgccg
     3061 aacggataga gcagcgcctt aaactgtgtt tcacgcccct gacgtcgcat cgccgcgcga
     3121 aaacgcagat gcgccaggca gatcataatc cagttcaaca gcaacgttgc taccaccagc
     3181 gccatcagca gaccaaacgc tttttgcggc agcagatagt tgattaacac caccagcgaa
     3241 gtgatcgctc cggaaagcat cagcgagtta atcggcacac cgcgacggct gacgcgagtc
     3301 aaaaacttcg gcgcattacc ctgcacagaa aggccaaaca gcatgcggct gttagagtaa
     3361 accccgctgt tatacaccga cagcgatgcc actagaatga cgaagttcag cgcagaagct
     3421 accacattgc tgtcgagatt atggaaaatc atcacaaacg ggctactgtt ggatttcact
     3481 tccacccacg gatagagtgc cagtaaaacc accagtgaac cgatataaaa cagcaggatg
     3541 cgatacacca cctgatttac cgcttttgga atgctttttt ccggatcgcg cgcttcagcg
     3601 gctgtaatcc caatcagctc cagaccgccg aaggagaaca taattaccgc cagcgacaaa
     3661 atcagcccat tccagccggt ggcgaagaaa ccaccgtagc gccagaggtt gtcgatactg
     3721 gctttctcgc cgccgtaacc agaaaacagc agccacaggc caaagccgat cataccgatg
     3781 atcgccagca ctttaatcag cgcaaaccag aactcggttt cgccatataa gcgcacgttc
     3841 accaggttaa cggcgttgat gataataaag aaggcggcag cccaaatcca cgttggaaca
     3901 tccgggaacc agtactgcat atagatgccc gcagcggtca gctctgccat tcccaccagc
     3961 acgaacatta cccagtagtt ccagccagag aggaagcccg caaacggtcc ccagtattta
     4021 taggcaaagt gggcaaatga accggatacc ggctcttcga ccaccatctc gccaagctgg
     4081 cgcataatca ggaaagcgat gatcccggcg acgccgtagc ccagcaatac agccggaccc
     4141 gccatctgaa tcgccgggcc aatgccaaga aacagaccag taccaattgc gccacccagc
     4201 gcaatcagtt gaatatgacg gttatgtaat ccgcgatgaa gcgtcggctc ttgattcgac
     4261 gcagtatctt ccgatacggt tgacgcgttt ttcacgcctt tcccctgtgt gtcttttttg
     4321 ttgaggggca ccttttaaca tttagtgccc atcgtcgcaa gacacaatcc acacggttaa
     4381 acgaggtatc ctgctttaat tccgtacccg atgtcggtgc agccacatca gcttatacgc
     4441 cgccgggata ataaacagcg acagcaaagg tgcggtgatc atgccgccaa tcatcggtgc
     4501 ggcaatccgg ctcatcacct ctgaaccagc ccccgttccc cacagaatcg gcagcagacc
     4561 ggcgataatc accgccaccg tcatcgcttt cgggcgcacg cgcaggaccg cgccgtgata
     4621 caacgcctca tccagcttct gctcgctgaa tgtttgtgga ttattcaacg acggctcggc
     4681 ctctatggcg tgacgtaaat acatcagcat caccacgcca aattcggcgg cgaccccggc
     4741 aagggcaata aagccggtac ccgtcgccac agaaagatga aagcccatcc accacagtaa
     4801 ccagatgccg cccaccagcg caaacggtac gctgctgata atcagcaacg cttcgcccac
     4861 ccgacggaat gccagataca acagcacgaa gatgatcatc agtgtcatcg gcaccatgag
     4921 tttcagctta tggttggcgc gctccagtag ctcgaactgc ccggagaatg ccacgctggt
     4981 gcccggtttt aactgcactt tttcagctat cgctttttgc aaatcgtgaa ccaccgacac
     5041 catgtcacga tcgcgggcat cgatataaat ccagctcgtc gggcgcgcat tctcggtttt
     5101 cagcatcgac ggtccggtag agactttaac gtcggccacg tctgccaggg tgatttgctg
     5161 cttcatcggc gtcaggatcg gcaactggcg cagtgcctgc ggactatcgc gccagctttg
     5221 cggataacgc agattaattg gataacgggc aatcccttcc accgtttcgc caaccatcgc
     5281 cccgcccacc gcagaagtca caaacaactg cacatccgcc accgtcatac cgtaacgtgc
     5341 ggctttttca cggttaatct caacgttgat atagcgccca ccttccagcc gctcagcaag
     5401 cgcagaagct acgcctggca ccgttcgcgc cacttcttca atttgctcag ccatcgcgtc
     5461 gatatccgcc agcacagtgc cggaaacttt aatgccgatg gggcttttaa tgccggttga
     5521 gagcatatcg atacggttac gaattggcgg cacccacaga ttcgccagcc ccggcagacg
     5581 cacggtgtta tccagttcct caatgatttt gtccattgtc atgcctggcc gccactgatc
     5641 ctgtggctta agctggatgg tcgtttctac catctccagc ggagcagaat cggtggcggt
     5701 ttccgctttc ccggttttgc caaatacccg cgccacttca ggtacgctca taattagctt
     5761 gtcggttttt tgcagcatac tcgccgcctc tgctgcggaa atccccggca gcgtcgatgg
     5821 catatacaac aagtcgcctt cattgatctg cggtaaaaat tccccgccaa ctttattgag
     5881 cggccagaga accgtcagca ccgaaagcgc cgccaccagc agcgtggttt tcggccagtg
     5941 cagtactttc agcaacagcg gatgataaac acgaatcaaa aagcgattga gcggattact
     6001 gctttccggc ggaattttgc cacggatcca gtaacccatc aggataggga tcactacgat
     6061 cgccagcagc gccgcacccg ccatcgcata cgttttggtg aacgccaacg ggccaaacag
     6121 acgcccttcc tgcccttcca gggtgaagat cgggataaac gacaacgtga taatcagcag
     6181 actgataaac agcgccggcc ccacttcaac agacgcatcg gtgatcacct gccagcgcgt
     6241 tttattatcc agcgtggcgt caggatgctg gtgctgccac tcttccagcc gtttatgcgc
     6301 attctcgatc atgacgatag cggcatcgac catcgccccg acggcaatcg caatgccacc
     6361 cagcgacata atattggcat tcagcccctg gaagggcatg acaataaaag caatacacaa
     6421 ccccagcggc aacgaaataa tcgccaccag cgccgagcgc acatgccaga gaaacagcgc
     6481 gcagactacc gccaccacaa taaactcttc cagcaacttg ccgctgaggt tgtcgatggc
     6541 ccggtcaatg agctggctgc ggtcgtatgt tgtaactatc tccacgcctt ccggcagact
     6601 acttttcagc gtttccagtt tgtccttcac ggcggcgatc acttctcggg cgtttttgcc
     6661 ggatcgcagg atcaccaccc cgcccgccac ttcgccttcg ccgtttagtt cggcaatgcc
     6721 ccggcgcatc ttcgggccaa tctggacctt cgcaacatcg cgcagataaa cgggcacgcc
     6781 attttcactg gcttttaaaa cgatgtgatt aaagtcgtcg agcgtttgta gatagccgct
     6841 ggcgcgcacc atatattctg cttccgccag ttcgattgac gaaccgcccg cttcctggtt
     6901 tgaagcatcc agcgcgcttt ttacttcggc gagactgatg ccatactgcg ccaggcgctg
     6961 aggatcgata accacctgat actctttcac cacaccgcct accgacgcca cttccgcaac
     7021 gtcaggaatg gttttcagct catatttgag aaaccagtcc tgcaatgagc gtaaatcggc
     7081 aagatcgtgc ttaccgctgc gatccaccag tgcatattca tagatccagc caacacccgt
     7141 ggcatctggt cccagctcgg cgctgactcc cgcaggcagc ttaccctgta cctggttgag
     7201 gtactccagc acccgcgaac gcgcccagta cggatcggtg ccatcttcga aaatgacata
     7261 cacataagag tcgccaaact gcgagaaacc acgcacagtc ttcgcgccag gcaccgacaa
     7321 catggtggtg gttagcggat aagtcacctg attttcaacg atttgcggtg cctggccggg
     7381 atagctggtt ttaataatca cctgcacatc ggagagatcc ggcaacgcat ccactggcgt
     7441 attaatgatg gtccaggtgc cccagatgct cagaaacaac gcgcccatca gcaccaaaaa
     7501 acggttcgcc accgagcgac gaataatcca ttcaatcatt ggttattccc tcaatgcgca
     7561 tgggtagcac tttcagagcg catccgctcc agtgcgccag aaatattggc ttcggaatca
     7621 atcaggaaca ggccgccgga aaccaccttt tcaccttccg ccagaccaga gcgtaatgcg
     7681 gtgacgcctt gcgatgcctg gaaaacagca acgcgtttcg gtacaaagcg cccgtcggca
     7741 tcaacggtaa tcacccgctg ttcgttgccg gtatcaatca gcgcctgtga cggaatgagc
     7801 agcatcggtt cgctggcggt gttgagttgc aaccaggcgt tcatgcccgg ttttagcgcc
     7861 tcgtcggcgt tgtcgacttc cagacgcagc tgcagcgtgc gggtcgcggc atccacgcca
     7921 ggtagcagcg tccatttgcg gatggtgagt gttttatccg gtcgcgccgg aacggtgagg
     7981 gtaaactgcg aggcatcttt caccagccag gcgatggact ccgggatcgc agcagtgacc
     8041 cacaccgggt ccataccctg aattcgcgat tacgcgcaat ccccctgaga accgccatga
     8101 acaagggttt catgatttgt gattttagtt tggtacgcaa tttggtacac aacactattt
     8161 tcacttcagc gggtagtcat caaactcacc ataacgagca tcgttgatga tgtatgtgat
     8221 caccacaaat atcacatctg gactagttcc gtcttcatgc gcaggtagac tggccccctg
     8281 aatctccaga caaccagtat cacttattta agtgatagtc ttaatactag tttttagact
     8341 tgtcattgga gaacagat
//
LOCUS       DAEEUE010000142         8243 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.142, whole genome shotgun sequence.
ACCESSION   DAEEUE010000142 DAEEUE010000000
VERSION     DAEEUE010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8243
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.142"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..71)
                     /locus_tag="KLK84_003675"
     CDS             complement(<1..71)
                     /locus_tag="KLK84_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE2010841.1"
                     /translation="MASVSISCPSCSATDGVVRNGKST"
     gene            129..377
                     /locus_tag="KLK84_003676"
                     /pseudo
     CDS             129..377
                     /locus_tag="KLK84_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
     gene            complement(746..1477)
                     /locus_tag="KLK84_003677"
     CDS             complement(746..1477)
                     /locus_tag="KLK84_003677"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010842.1"
                     /translation="MHRLDEVVDTLLVLQKKHRIRFDVWQVVKRDHAIISFFDQGMNP
                     AVPRVAYWTPFRYPLLLNLASLFDNELAEKAWCARLEAHDGRSSSLFSEVCSELLARV
                     HTLGDRRYIELITDALSWAMTHFDELGYNCKTGKQKLQIMPNMVGFQFVLRGICSRLG
                     APNRKADIVVDQQSQFNTTQRELREFYYQIREMPWVHGPGLPVMDVTNMPAERWFSSR
                     VQKVQGWSWLIYISGSSNALWKGRS"
     gene            2263..3240
                     /locus_tag="KLK84_003678"
     CDS             2263..3240
                     /locus_tag="KLK84_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBE2010843.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(3525..4265)
                     /locus_tag="KLK84_003679"
     CDS             complement(3525..4265)
                     /locus_tag="KLK84_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBE2010844.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLNGVRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLR"
     gene            complement(4386..4568)
                     /locus_tag="KLK84_003680"
     CDS             complement(4386..4568)
                     /locus_tag="KLK84_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HBE2010845.1"
                     /translation="MHVAQLPSLCIFLLSLRSSLPWHYPISPKNGKPALSPRHDWLNT
                     GSLLQNIPLRIWFYPQ"
     gene            complement(4911..5218)
                     /locus_tag="KLK84_003681"
                     /pseudo
     CDS             complement(4911..5218)
                     /locus_tag="KLK84_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461876.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
     gene            5711..6880
                     /locus_tag="KLK84_003682"
     CDS             5711..6880
                     /locus_tag="KLK84_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010846.1"
                     /translation="MNQKWKTLIISLLTPSSIISGIALIVLWGYFSRLGRLDIFFDVM
                     NIKSILVLVCCATILSLAMIVFIFFITSFFIPVVIPQDINNLPAYNKIQGNFLSVMML
                     SGMFPMAFIYVLYCAFDFDQNVKDNSGWLSIASMGVLIAVILAIVNKRYLEYDLSFKN
                     NRMKLLRRVQIYLVIPLSIALLVHLQLIPLEIVFSNIATSDKSVNFKVIAELAFMSYF
                     IFLLTMLPGVIYLKMNPQHKFSKRISYSFIASLMLLLIISTQITVLPVIFTHSVIKLS
                     GISDFKIHSYIIKTSEYPEEFFSNAAWDKKNIKPGDYYSVQAVSMFTTNQFILLCPKD
                     IIKFYRESWKFELLNVDFDINTRKKLQEEAAYCVPISAISVKRWDMPLQGSKPSS"
     gene            6913..7041
                     /locus_tag="KLK84_003683"
                     /pseudo
     CDS             6913..7041
                     /locus_tag="KLK84_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(7076..7369)
                     /locus_tag="KLK84_003684"
     CDS             complement(7076..7369)
                     /locus_tag="KLK84_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010847.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSIAVAEMFHNAAWGARTLW
                     FELVTKIDIDIHKKNRYASYDLRRKIEMQHEEFQKMTEREQVPLLKCISSDLI"
     gene            complement(7475..7750)
                     /locus_tag="KLK84_003685"
     CDS             complement(7475..7750)
                     /locus_tag="KLK84_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBE2010848.1"
                     /translation="MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIYVLRLWHTRENR"
     gene            complement(7750..8034)
                     /locus_tag="KLK84_003686"
     CDS             complement(7750..8034)
                     /locus_tag="KLK84_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HBE2010849.1"
                     /translation="MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWSDSLSTDNPLPVPR"
BASE COUNT         2182 a         1924 c         1873 g         2264 t
ORIGIN      
        1 gtgcttttgc cgttacgcac caccccgtca gtagctgaac aggagggaca gctgatagaa
       61 acagaagcca caggagcacc tcaaaaacac catcatacac taaatcagta agttggcagc
      121 atcaccgtac aaatgataat gttccaggct tgctttccct gataactgca catctcaaag
      181 acctgcctga tgatggaaga aatgaagatg tgtttaaaat gttgagatca tctgccgcaa
      241 tacttcatgg gatcaataac ctgcgtaaca attacagcat ggcccatcct actgaaactt
      301 tgctgaatga agccgatgcc cgttttgcca taaatctggt tcgttccatc atgacttatg
      361 ttgatgagtt gctttagttt taataaaaat atgagactca cagcgaacag atagaacttc
      421 aacgaagaag tgaagtacgg ccagaatttc gagctctggc cctccggaag atgccggaca
      481 gacgcatcat cccgtcgtgg ccgcttccat ttttgtgtgt actcaacctg ccggatcagg
      541 atggcggcag gatttgcacc cgatgttcca gccgccttgc ctcttcctga tcccggtatt
      601 ccctcgcctt tttcatcatt tctgccgttg gctcctgcag tttgtcgaga aactccttag
      661 atcgtttagc caccgactga agggagacac tgtccgtcct gccggtattg cgattggtgt
      721 aaaccaggcg ggtaaggggg tctggttaac tccttccctt ccataaagcg tttgaagatc
      781 cagagatata tatcaaccag ctccagccct gcactttttg tacccgactg gaaaaccagc
      841 gttcagccgg catgttcgtc acatccatga ccggtaaacc gggaccgtgt acccacggca
      901 tctcacgtat ctggtaatag aactccctca gctcccgctg ggtggtgttg aactgtgact
      961 gctggtccac aacgatgtcg gctttgcgat ttggtgctcc caggcgggaa cagataccgc
     1021 gcaatacgaa ctgaaatcca accatattgg gcatgatctg cagcttttgt ttaccggttt
     1081 tgcagttata tcccagttcg tcaaagtggg tcatcgccca ggacaacgca tcggtaatca
     1141 attcaatgta acggcggtcc cctagggtat gaacgcgcgc aagcagctca ctgcagacct
     1201 cactgaacag agatgaggac cttccatcat gggcttcgag gcgggcacac cacgcttttt
     1261 cagccagttc attatcgaac aggcttgcca gattcagtag cagcgggtaa cgaaaggggg
     1321 tccagtaggc aaccctgggc actgcaggat tcatcccctg atcgaagaac gaaataatgg
     1381 cgtgatcccg tttaacgacc tgccagacat caaaccgtat ccggtgcttc ttctgcagga
     1441 caagcagcgt atcaaccacc tcatcaaggc ggtgcatgcc aagctctgcg gcgtgcagac
     1501 gctgtacttg tcaacgccac gatctttgac cgtcttatat cgacagagtt atccggcatg
     1561 gtgtcaatcc gttcacaatt tttccatcgg agcagcctac caccggtgat aaccagactt
     1621 tgcctgtagt ttgtatacac aatccctggc attgaaacac acatatgaag tgtacttcgg
     1681 atatgaccgt acgctcagga agtacggcag attccgtcaa aaattacctg taaagacaga
     1741 catcagaaaa acagtgctcg ccgcagaccg gtgaccgggc gaagcccgcg aaccggcgag
     1801 gaagcgcaat ggatctgtag ttacagtgag caggtacgcc ctgaagcctt cataatcggt
     1861 agtgtatgct gtttttctgg aatgatttat ccacatatcc ggaggcccga tccagtaaac
     1921 agatccatga atgatcaaca aaggatccat taaagatccc catatctctg caaaccttgt
     1981 cactcatggg ccgggactac gatcacataa gctgtggcat gttactgata aactgtaaca
     2041 tgctaatgat aagctgtatt cagtaatcca tatactgaag taagttaatg acataaacta
     2101 tggtcagtat gccagactca gttgttaaat acaggctgca ggtttttctt cagtcagtta
     2161 gcggagttct gacacacgat tttctgttta ttcttttact gtccacaggc tggaggcttt
     2221 ctggaaaacg aaaattcaga catcaaaaaa ctgttcggcg aggtggataa gtcgtccggt
     2281 gagctggtga cactgacacc aaacaataac aacaccgtac aacctgtggc gctgatgcgt
     2341 ctgggcgtct ttgtaccgac ccttaagtca ctgaagaaca gtaaaaaaaa tacactgtca
     2401 cgcaccgatg ccacggaaga actgacgcgt ctttctctgg cccgtgctga gggattcgat
     2461 aaggttgaga tcaccggccc ccggctggat atggataacg atttcaagac ctgggtgggg
     2521 atcattcatt cctttgcccg ccataacgtg attggtgaca aagttgaact gccttttgtt
     2581 gagtttgcaa aactgtgtgg tataccttca agccagtcat cccgcaggct gcgtgagcgc
     2641 atcagccctt ccctgaagcg cattgccggt accgtgatct cgttttcccg taccgatgag
     2701 aagcacaccc gggaatacat cacccatctg gtacagtcag cctactacga tactgaacgg
     2761 gatattgttc agttacaggc tgatccccgc ctgtttgaac tgtaccagtt tgacagaaag
     2821 gtccttcttc agcttaaggc gattaatgcc ctgaagcgac gggagtccgc ccaggcactc
     2881 tacaccttta tagagagcct gccccgggat ccggcaccga tatcgctggc gcggctgcgt
     2941 gcacgcctca atctgaagtc tcctgtattt tcccagaacc agacggtcag acgggcaatg
     3001 gagcagctgc gcgagattgg atatcttgat tacacggaga tccagcgggg gcggacaaaa
     3061 ctcttctgca ttcactaccg gcgtccccgg ttaaaagcac cgaatgatga gagtaaggaa
     3121 aatccgttgc caccttcacc tgcggaaaaa gtcagtccgg agatggcgga gaaacttgcc
     3181 ctgcttgaaa aactgggcat cacgctggat gacctggaaa aactcttcaa atcccgctga
     3241 acataaactg tagtcagtga agagcgctcc tttactgact acagcttata ttaaccggtg
     3301 cagtgagtgg tctgctcact gcagtttata ttctgtttcc tgcagtgctg cctgtggctg
     3361 agctgccatc tgcctgtccc ttacgtaagt gatcccgtaa cctgctgctg aggcattgct
     3421 cccttcataa aacatgactt actcactaca gcttatatac atgctccagc ttatgttatg
     3481 tctgttctgc tgaccacagc ttatataagg aagcgcgtat gccgtcacct caggggaggc
     3541 agtgtacgca ggatctccgc agcatcccgc ccgtcacctg tgaaaggcac tgccagcgtg
     3601 gcagccatat ccagtgcaaa cactctggta taaacctcca tcgaacgtgg atccctgtga
     3661 ccagccagtg cctggatgac tttccggggc tggcggtgat agagcatgtg catgatatag
     3721 ctgtgccgga aggtgtgtgg tgtgaccgga atcgaaaagt gtaccccgtc tgcttcggcc
     3781 cgtctgacag cctgcttcag ccagttgcgc atggtttcgt cggtcacagc ccataatggt
     3841 tcacgacgac ggggccgggt ggtgatcatc cagctttcca tctgcctgac atagcttata
     3901 tctgtcagcg gaaccaggcg gacttcatct tttggcgggc gtccgcgtcg cgcacgcact
     3961 ttttcggaca ggatccgcac aaacggtctt actccattca ggtcaaatga ttccggcgtc
     4021 agcatccggg cttcgccaat acgcattccg gtattccaga gggtggcgaa cagcatatgg
     4081 tgacgctgat ccggcatata gaagagaagg gcactcactt ccggggccag caggtaggcc
     4141 ggtgtggccc ccgtggaagt ggccattctt ctcagggaga gcgctgttgc aaaatccacc
     4201 cccggcgcaa tgggtaacag ggagacgcgt tctggtgaat tctgcagggg aatgacattg
     4261 ttcatgagtt atttttcacg tagcacacgc actgtttatg gatacaggta ttctgacctg
     4321 aacgcccgga tccttccacg acaataaatc gcagcgcgcg gatccttttg tttgcttatc
     4381 acgcattatt gcgggtaaaa ccaaatacgt aaaggtatgt tttgcagcag ggatcccgta
     4441 ttaagccagt cgtggcgcgg gctgagggct ggtttgccgt ttttgggaga aatggggtaa
     4501 tgccagggca aagatgatcg caaggaaagg agaaaaatac acagagaggg gagttgcgct
     4561 acgtgaattc tggctaggta cacccctgta accctgccag ttttcacgca acgcaatgtg
     4621 gtggctgatt actgtcagtt gaaatgacgg ctggcatcct gatgctgact taatatacga
     4681 ataataacaa tttcagtatc agtctgcaga aaataaacca tatgccgttc aacgggtagt
     4741 gcgaatgtgt attccccagt tcaggagggg gagtaccgat attatgatta ctcataacct
     4801 gaaaaatgtc ggatatctga ttaatatatt tatcagccta ctcttcaccg aaatggtggt
     4861 acccgtaatg ctagatatcc tccagatcct gactggcttt aggtgtgagt ttaacggttt
     4921 tcaccggaag ccctcgcgcc agctttcact tttttcagaa acgcattttt ttcccacgac
     4981 acaggttcac cgctgctcaa gccttctgcc agtaaatccc gcaggacacc tggagacgtg
     5041 actctgcctg tttctctcga aggagacgaa gtgactcgcg gattacctca ctctgtgtac
     5101 gataatcacc tgattctatg agagattcta tgtactccca gagttcatct ccgagagcaa
     5161 ctgtcattgt tctggccata tcaagcctcg tgattgtaag tttaaatttt acatgcagtt
     5221 tattcgatac tcaaaggtgc ttccagtaca gaacatttgg gagaattggg gtaatgcctg
     5281 gaacagacag atacggagaa aaaccctgca gataacctta tacgacaact gaaggtgtct
     5341 gatgccatat tcttagctac ttctctgtct tcaggcggct tagtcacaac ataagaccga
     5401 tgcataataa gcatattttt tcatgttatg gtcttgttca tgcatgaact attatctatt
     5461 cagtcacttt taaagtttta tatcatactg catatgatat atatcatctc ctatattcag
     5521 actaaaaata ttatgacgat ggtcaaatga atttagtgaa aacgctacct aaaacaatat
     5581 gcttatgata taacttatca agaatatgcg acaatcttct tgaaaggtta cggagaagag
     5641 tcagataatg aagtgttata actcattcag cttttttatg ttctctttat agtaaaacca
     5701 ggagattaag atgaatcaga agtggaagac gttaattatt tcattattaa caccatcaag
     5761 tattatatca gggattgcgt tgatagttct ctggggatat ttcagtcgcc tgggccgact
     5821 agatattttc tttgatgtaa tgaatattaa aagcatcttg gtattggttt gttgtgcaac
     5881 tatactatca ttggctatga tagtatttat cttttttata acctcttttt ttatacccgt
     5941 agttattcct caggatataa ataatcttcc tgcttacaat aagattcagg gtaatttttt
     6001 atctgtgatg atgttatctg gcatgttccc catggccttt atatatgtgt tatactgtgc
     6061 atttgatttt gatcagaatg ttaaagataa ctctggctgg ttatctattg caagtatggg
     6121 tgtactgata gctgttattt tagccatagt aaataagagg tatcttgaat atgacttatc
     6181 gtttaaaaac aatagaatga aactgttgag acgtgttcag atctatcttg tcattccgtt
     6241 gtctattgct ttactggtgc atcttcagtt aattcctctg gagatagttt ttagtaatat
     6301 tgcaacttca gataagagtg ttaatttcaa agtgatagct gaactggctt tcatgtcata
     6361 ttttatcttt cttctgacaa tgcttcctgg agtcatttat ctgaaaatga atccacagca
     6421 taaattttca aaaagaatta gctattcatt tatcgcatcc ttgatgttat tattaataat
     6481 atcgactcaa ataaccgtgt taccagtgat ttttacccat tccgttatta agctatcagg
     6541 tataagtgac ttcaaaatac atagctacat tattaaaaca agtgaatatc ctgaagagtt
     6601 tttttccaat gcagcatggg ataaaaaaaa tataaaaccg ggggattatt actctgtgca
     6661 ggctgtttct atgtttacga caaaccagtt tattctcctt tgtcctaagg atattataaa
     6721 gttttatcgg gaaagctgga agttcgagtt gcttaatgta gacttcgata taaataccag
     6781 gaagaaactc caggaagaag ctgcatattg cgtaccaatt tctgcaatat ctgtaaaacg
     6841 atgggatatg cctcttcagg gctcgaaacc ctcaagttag aaggcaacta gggcgactgt
     6901 attctgtgac gaaatgaatg gctggctcac tataataatg agcgaaccca tcagggaaaa
     6961 atgtgcaatg ggcggacgcc aatggaaacg ttactcgatg gaaaacgcat ctgggccgag
     7021 aaaaatttaa gccagatgta atctgacaga tacctgtata aataaccgat aactgtcaga
     7081 tcaggtctga gctaatacat ttcaataaag gtacttgctc gcgctctgtc attttctgaa
     7141 actcttcatg ctgcatttcg attttccttc taagatcata actcgcatac ctgttctttt
     7201 tatgtatatc tatatctatt tttgtgacga gttcaaacca caatgttcgc gctccccatg
     7261 cagcgttatg aaacatttcc gcaacggcta tagagtctgt attaactgct tcagtcatgt
     7321 agtcgctaat acgttcagcg gtaattttcg ttatttcatc gtaattcatt ttatctccta
     7381 caagattatt taatggaatg caaaatatct ggtctgataa taatgttcca gaattaatat
     7441 ctgcaggcta tgttaagttg tccccacaca caatctacct gttttctcgt gtgtgccaca
     7501 gacgcaatac ataaatagtc tggccattaa tttcgtaacg gatttcgtac tcgccagcaa
     7561 aaatccgtct gacctccctg ggttcaaact gaaacaactg ttctcccata cgtggatgag
     7621 ttaacagaat gaccggtgcc tgtgtcaggg actgtaccgt tctggcggca gcaggtttac
     7681 tggccagcac cagaaaatca aataaccgcg acaaatcaga aagcgcctta ctggtccact
     7741 tcagttccat cagcgtggca ccggtaacgg attgtcagta ctgaggctgt ccgaccaggc
     7801 ctgtacagcc tgatggtcga taacctgtcc ggatgtcaca tcgtccaggg cttccagcgt
     7861 caggcgatta cgctcctcct cctgggcaag ccatgcagaa agcgcctgtt tgataaccca
     7921 gccccgggaa cgttccagtc tggcggccat ctggtcgact ttatcagcca taggtaatgg
     7981 cacatgcgcg gtaattactt ttgttgcttg tgcggcattg tttttcattt gcatatccga
     8041 ttagttgtta atcatattta atcatagtga ttactttcag acggaacaag gcaaataatg
     8101 cgcgtcagcg catgtaaggc ggcatacgga ggttacatgt agcacaggtg taaaaccgcc
     8161 gaagcccggc gtaagccggt actgattgat agatttcacc tggcactgtt gcaaatagtc
     8221 ggtggtgata aacttatcat ccc
//
LOCUS       DAEEUE010000143         8037 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.143, whole genome shotgun sequence.
ACCESSION   DAEEUE010000143 DAEEUE010000000
VERSION     DAEEUE010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8037
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.143"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            52..585
                     /gene="ymdB"
                     /locus_tag="KLK84_003687"
     CDS             52..585
                     /gene="ymdB"
                     /locus_tag="KLK84_003687"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HBE2010850.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            527..2008
                     /gene="clsC"
                     /locus_tag="KLK84_003688"
     CDS             527..2008
                     /gene="clsC"
                     /locus_tag="KLK84_003688"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HBE2010851.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2016..3173)
                     /gene="mdoC"
                     /locus_tag="KLK84_003689"
     CDS             complement(2016..3173)
                     /gene="mdoC"
                     /locus_tag="KLK84_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HBE2010852.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            3567..5102
                     /gene="mdoG"
                     /locus_tag="KLK84_003690"
     CDS             3567..5102
                     /gene="mdoG"
                     /locus_tag="KLK84_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HBE2010853.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5095..7638
                     /gene="mdoH"
                     /locus_tag="KLK84_003691"
     CDS             5095..7638
                     /gene="mdoH"
                     /locus_tag="KLK84_003691"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HBE2010854.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         1998 a         1925 c         2111 g         2003 t
ORIGIN      
        1 accaaaaagc ctgctgtaca cttaagaaac aagaaggtgt aaggaggcgt tatgaaaacg
       61 cgtattcatg ttgtgcaggg tgatattacc aaactggccg ttgatgtgat tgtgaatgcg
      121 gctaatccgt cattaatggg aggcggcggc gtcgatgggg ccattcatcg cgcagcgggt
      181 ccggccctgc tggatgcctg tttaaaagtc aggcaacagc agggcgattg ccctacgggg
      241 catgccgtta ttacgcttgc aggcgatctt cccgctaaag ccgtagtaca caccgtcggg
      301 ccagtctggc gtggtgggga acaaaacgaa gaccagcttt tgcaggatgc ctatctgaat
      361 agcctacgac tggtggcggc aaacagctat acgtcagtgg cttttcctgc aatcagtact
      421 ggggtttata gttaccctcg tgcggcagcg gctgaaatcg cagttaaaac cgtttcagaa
      481 tttattaccc gtcacgcttt acccgaacag gtatactttg tctgttatga tgaagaaaac
      541 gcccacctct acgaaagact ccttacccaa caaggagatg aatgatttgc cccggctggc
      601 gagcgcggtg ctgccactgt gttcgcaaca tcccggtcag tgtggtcttt tccctctgga
      661 gaaaagtctg gatgcgtttg ccgcccggta tcgtctagcc gaaatggcag agcatacgct
      721 ggatgttcag tattacatct ggcaggacga tatgtcgggg cggttactgt tttccgccct
      781 gttagccgca gcaaagcgtg gcgttcgcgt ccgtttgttg ctggacgaca acaatacgcc
      841 cggacttgac gacattttgc gcttgcttga cagtcatcca cgcattgaag tccggctttt
      901 taatcctttc tcgtttcgct tgctacgtcc gcttggatat atcaccgact tttcccgtct
      961 caatcgccgg atgcacaata aaagtttcac tgtcgatggc gtggttaccc tagtgggagg
     1021 acgaaatatt ggtgatgcct attttggtgc aggggaagag ccactttttt cggatttaga
     1081 tgtcatggca ataggccccg tggtagagga tgttgccgat gatttcgccc gctactggta
     1141 ttgcaaatcg gtttcaccct tacagcaggt gctggatgtc ccggaaggtg aaatggcgga
     1201 tcgcatcgag ttacccgcct cctggcataa cgatgccatg acgcatcgtt atttacgcaa
     1261 aatggaatcc agtccattta taaatcatct ggttgatgga acgttgccgc ttatctgggc
     1321 gaagacacgt ttattaagtg atgatccggc gaaaggggag ggcaaggcaa aacggcattc
     1381 actgttaccg cagcgcctgt tcgatatcat gggctcaccc agtgaacgca tcgatattat
     1441 ctcttcctat tttgtaccga cacgcgcagg tgtggcgcaa ctcttacgga tggtgagaaa
     1501 aggggtaaag attgcgatcc taaccaattc tcttgccgct aacgatgttg ctgtcgtcca
     1561 tgccggatac gcgcgctggc gcaaaaaatt gctccgctat ggcgtggaat tatatgaact
     1621 caagccgacg cgtgaacaaa gcagtacgtt acacgatcgc ggcataaccg gtaattccgg
     1681 tgccagcctg catgctaaaa cttttagcat cgatggtaaa acggtgttta tcggctcttt
     1741 caatttcgat ccgcgttcaa cattgctcaa tactgaaatg ggcttcgtga tagagagcga
     1801 aacgctggca cagttaattg ataaacgctt tattcagagc cagtacgatg cggcctggca
     1861 gctccgtctg gacaggtggg gacggatcaa ctgggttgat cgtcatgcaa agaaagaggt
     1921 tattctcaaa aaagaacccg ccaccagttt ctggaagcgg gttatggtca gactggcgtc
     1981 gatattgccc gtggaatggt tattgtaaat gtggcttaac gggctggtgc tttatcgttc
     2041 tcacgcttaa caaccggttt accagagaac aaaaacttca gtaacgggat gcgtaaatga
     2101 atttcataca gaattatcgc aatccctact acgaatatca ggccacagag aaaaccaagc
     2161 cagttggagg tgatgtgcgg tgtaatgtat gcgccgaaaa acagcgttaa cgggtggtga
     2221 accagataga taaacagcga tgcgttaaca aaataagtca cccgcgctga ctggaagtta
     2281 agcaaacggt ggccaaagga gaagaccaca ttcaccatcc acaggccgag gaccatggtg
     2341 atcaccgact cggtttcgta catccaggca tcgccactgc catagcgctg gttgagtaaa
     2401 taagcgacaa acgccaatgc tgcagcgagg gtacagccac gagacggcgt ggtaaacaag
     2461 gctttaagat gagggaaaat gaaagccagt gcgccgagga taaagaacgg taaataaaat
     2521 agcgtttgca tgacaataaa attgaacatg ccattactca gaatgggtgg atacacaata
     2581 aaaatcgttc ttcttattac cgcatagccg atgccgaggc ataaaaaaat caccgatagt
     2641 tttaccatcg agaatttttt attcgtttta tcagaatttt ctaaattatt tctgatgcgc
     2701 ttaaatatcc atacacacag cgtcgtcatg actactaaca ccagtaaaaa ccacaggtgt
     2761 gatattaatt cccaggccaa cgtattatat ttgtcataca atgatagccc aggccaactt
     2821 tccgctttcc ctttgacata ttgcagcata ataaattgcg gtaatgtcag tagggggatg
     2881 gctgttaaca tcgggatacc tacacgttcg acacgtactt tccaccattt tttcaaggga
     2941 tagcgtaaaa aaagcatgta ggaaaagtat ccggatataa cgaaaaatac ctgcatgcgg
     3001 aacgagtgga tgaagtcatt aaaaagggtt agccacaatg acggttcggc gctattcaca
     3061 tgccatgtat ggctcgaata gattaaagaa atatgaaaag ggatccctaa caacatcagc
     3121 caggcgcgga tggagtcgag gaaatattca cgttgcgcgg gtactggatt catatatggt
     3181 taactaatct cggatttttc gtcttatccc tgtcgggtta tgcctttagg cttgttgcca
     3241 tagcgacacc gacctgaccg cgccaggcgc aggcttcaag gtttttatgc atagcatcat
     3301 cgctaccact aaccagaatg gaagcgtctg taagacggtt gataaataaa tttgctggca
     3361 aaccctacac gaagtcgatg cttctgtctt taggagaagc acggaaagtg aaaacggttg
     3421 caatcaggtg cttaatccat gagccagcgt gctgaacgat accgggattc agttgtcgga
     3481 atggctggtt atccattaaa atagatcgga tcgatataag cacacaaagg gggaagtgct
     3541 tactaattat gaaacataaa ctacaaatga tgaaaatgcg ttggttgagt gctgcagtaa
     3601 tgttaaccct gtatacatct tcaagctggg ctttcagtat tgatgatgtc gcaaagcaag
     3661 ctcaatcctt agccgggaaa ggctatgagg cgcctaaaag caacttgccc tccgttttcc
     3721 gcgacatgaa atacgcggac tatcagcaga tccagtttaa tcatgacaaa gcgtactgga
     3781 acaatctgaa gaccccattc aaactcgagt tctaccatca gggtatgtac ttcgataccc
     3841 cggtcaaaat aaatgaagtg actgccaccg cagtcaaacg aatcaaatac agcccggatt
     3901 atttcacttt cggcgatgtt cagcatgaca aagacacggt aaaagacctt ggttttgccg
     3961 gttttaaagt gctttacccg atcaacagca aagataaaaa cgatgaaatc gtcagcatgc
     4021 tcggggccag ctatttccgc gtgattggtg caggtcaggt ttatggcctt tctgcccgcg
     4081 gcctagcaat tgataccgct ttgccatcgg gtgaagaatt tccacgcttc aaagagttct
     4141 ggatcgagcg tccaaaaccg actgataaac gtttaaccat ttatgcattg cttgactcgc
     4201 cgcgtgcgac aggtgcttac aaattcgtgg ttatgccagg gcgtgacacg gttgtggatg
     4261 tgcagtcgaa aatctatctg cgcgataaag tcggcaaact gggggttgca ccgttaacca
     4321 gtatgttcct gtttgggccg aaccaaccgt cgcctgcaaa taactatcgt ccggagttgc
     4381 acgactctaa cggtctgtct atccatgctg gtaatggcga atggatctgg cgtccgttga
     4441 ataacccgaa acatttagcg gtcagcagct tctccatgga aaacccgcga ggctttggtc
     4501 tgttgcaacg aggtcgtgat ttctcccgct ttgaagatct cgatgatcgt tacgatctcc
     4561 gtccaagcgc atgggtgact ccgaaagggg agtggggcaa aggcagcgtt gagctggtgg
     4621 aaattccaac caacgatgaa accaacgata acatcgtcgc ttactggacg ccggatcagc
     4681 tgccggagcc gggtaaagag atgaacttta aatacaccat caccttcagc cgtgatgaag
     4741 acaaactgca tgcgccagat aacgcatggg tgcaacaaac gcgtcgttca acgggggatg
     4801 tgaagcagtc gaacctgatt cgccagcctg acggtactat cgcctttgtg gtcgatttta
     4861 ccggcgcaga gatgaaaaaa ctgccagagg ataccccggt cacagcgcaa accagcattg
     4921 gtgataatgg tgagatagtt gaaagcacgg tgcgctataa cccggttacc aaaggctggc
     4981 gtctggtgat gcgtgtgaaa gtgaaagatg ccaagaaaat cactgaaatg cgtgctgcgc
     5041 tggtgaatgc cgatcagacg ttgagtgaaa cctggagcta ccagttacct gccaatgaat
     5101 aagacaactg agtacattga cgcaatgccc atcgccgcaa gcgagaaagc agcattgccg
     5161 aagactgata tccgcgccgt tcatcaggcg ctggatgccg aacaccgcac ctgggcgcgg
     5221 gaggatgatt ccccgcaagg ctcggtaaag gcgcgtctgg aacaagcctg gccagattca
     5281 cttgctgatg gacagttaat taaagacgac gaagggcgcg atcagcttaa ggcgatgcca
     5341 gaagcaaaac gctcctcgat gtttcccgac ccgtggcgta ccaacccggt aggccgtttc
     5401 tgggatcgcc tgcgtggacg cgatgtcacg ccgcgctatc tggctcgttt gaccaaagaa
     5461 gagcaggaga gcgagcaaaa gtggcgtacc gttggtacca tccgccgtta cattctgttg
     5521 atcctgacgc tcgcgcaaac tgttgtcgcg acctggtata tgaagaccat tcttccttat
     5581 cagggttggg cgctgattaa tcctatggat atggttggtc aggatttgtg ggtttccttt
     5641 atgcagcttc tgccttatat gctgcaaacc ggtatcctga tcctttttgc ggtactgttc
     5701 tgttgggtgt ccgccggatt ctggacggcg ttgatgggct tcctgcaact gcttattggt
     5761 cgcgataaat acagtatatc tgcgtcaaca gttggcgatg aaccattaaa cccggagcat
     5821 cgcacggcgt tgatcatgcc tatctgtaac gaagacgtga accgtgtttt tgctggcttg
     5881 cgtgcaacgt gggaatcagt aaaagccacc gggaatgcca aacactttga tgtctacatt
     5941 ctgagtgaca gttataaccc ggatatctgc gtcgcagagc aaaaagcctg gatggagctt
     6001 atcgctgaag tcggtggcga aggtcagatt ttctatcgcc gccgccgtcg ccgcgtgaag
     6061 cgtaaaagcg gtaatatcga tgacttctgc cgtcgctggg gcagccagta cagctacatg
     6121 gtggtgctgg atgctgactc ggtaatgacc ggtgattgtt tatgcggcct ggtgcgcttg
     6181 atggaagcca acccgaacgc cgggatcatt cagtcgtcgc cgaaagcgtc cggtatggat
     6241 acgctgtatg cgcgctgtca gcagttcgcg acccgcgtgt atgggccact gtttacagcc
     6301 ggtttgcact tctggcaact tggcgagtcg cactactggg gacataacgc gattatccgc
     6361 gtgaaaccat ttatcgagca ctgcgcactg gctccgctgc cgggcgaagg ttcctttgcc
     6421 ggttcaatcc tgtcacatga cttcgtagaa gcggcgttga tgcgccgtgc aggttggggg
     6481 gtctggattg cttacgatct cccgggttct tatgaagaat tgccgcctaa cttgcttgat
     6541 gagctaaaac gtgaccgccg ctggtgccac ggtaacctga tgaacttccg tctgttcctg
     6601 gttaagggta tgcacccggt tcaccgtgcg gtgttcctga cgggcgtgat gtcttatctc
     6661 tccgctccgc tgtggtttat gttcctcgcg ctctctactg cattgcaggt agtgcatgcg
     6721 ttgaccgaac cgcaatactt cctgcaacca cggcagttgt tcccggtgtg gccgcagtgg
     6781 cgtcctgagc tggcgattgc actttttgct tcgaccatgg tgctgttgtt cctgccgaag
     6841 ttattgagca ttttgcttat ctggtgcaaa ggaacgaaag aatacggcgg cttctggcgc
     6901 gttacattat cgttgctgct ggaagtgctg ttttccgtgc tgctggctcc ggtacgcatg
     6961 ctgttccata cggtcttcgt tgtcagcgcg ttccttggct gggaagtggt gtggaattca
     7021 ccgcagcgtg atgatgactc cacttcatgg ggcgaagcgt tcaaacgcca cggctcacag
     7081 ctgctgttgg ggttagtgtg ggctgttggg atggcgtggc tggatctgcg tttcctgttc
     7141 tggctggcac cgattgtctt ctcgttgatc ctgtcaccgt ttgtttcggt gatttccagc
     7201 cgtgccaccg ttggtctgcg caccaaacgc tggaaactgt tcctgatccc ggaagagtat
     7261 tcaccgccgc aggtgctggt tgataccgat cggttccttg agatgaatcg ccaacgctcc
     7321 cttgacgatg gctttatgca cgcagtgttt aacccgtcat ttaacgctct ggcaaccgca
     7381 atggcgacag cgcgtcaccg cgccagtaag gtgctggaaa tcgcccgtga ccgccacgtt
     7441 gaacaggcgc ttaacgagac gccagagaag ctgaatcgcg atcgtcgcct ggtgctgcta
     7501 agcgatccgg tgacgatggc ccgtctgcat ttccgcgtct ggaattcccc tgaaagatat
     7561 tcttcatggg tgagttatta cgaagggata aagctcaatc cactggcatt gcgtaaaccg
     7621 gatgcggctt cgcaataaaa acgtagttgc ctgatgcgct acgcttatca ggcctacatc
     7681 gttcctgcaa tttattgatt ttgcaagatt ttgtaggtcg gataaggcgt tcacgccaca
     7741 tccggcaaaa acaacgagcc aataaaaata ccggcgttat gccgtaatgc cagtcagtta
     7801 agcaactgac tggctctttt tcggggctgt ggggtatttc cagggcctct cctttaccac
     7861 tctcgatcga taggaattaa aaccccaaaa agattaaaaa aacaccacaa aacggatgtt
     7921 tcttcaacac cacttttgct ccatatgaac ggaaccgacg attaaactgg atggctctga
     7981 ttgattcagg gtatgaatgg cggttttttg ctccgtttcc ctcaaaatgg acgcaac
//
LOCUS       DAEEUE010000144         7931 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.144, whole genome shotgun sequence.
ACCESSION   DAEEUE010000144 DAEEUE010000000
VERSION     DAEEUE010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7931
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.144"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(280..355)
                     /locus_tag="KLK84_003692"
     tRNA            complement(280..355)
                     /locus_tag="KLK84_003692"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(320..322),aa:Asn,seq:gtt)
     gene            complement(456..2083)
                     /gene="yeeO"
                     /locus_tag="KLK84_003693"
                     /pseudo
     CDS             complement(456..2083)
                     /gene="yeeO"
                     /locus_tag="KLK84_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            2088..2163
                     /locus_tag="KLK84_003694"
     tRNA            2088..2163
                     /locus_tag="KLK84_003694"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2121..2123,aa:Asn,seq:gtt)
     gene            complement(2201..3151)
                     /gene="cbl"
                     /locus_tag="KLK84_003695"
     CDS             complement(2201..3151)
                     /gene="cbl"
                     /locus_tag="KLK84_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HBE2010855.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLEFCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(3253..4170)
                     /gene="nac"
                     /locus_tag="KLK84_003696"
     CDS             complement(3253..4170)
                     /gene="nac"
                     /locus_tag="KLK84_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HBE2010856.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKIIYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4498..4573
                     /locus_tag="KLK84_003697"
     tRNA            4498..4573
                     /locus_tag="KLK84_003697"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4531..4533,aa:Asn,seq:gtt)
     gene            complement(4629..5564)
                     /gene="ldtA"
                     /locus_tag="KLK84_003698"
     CDS             complement(4629..5564)
                     /gene="ldtA"
                     /locus_tag="KLK84_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBE2010857.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(5626..6705)
                     /gene="cobT"
                     /locus_tag="KLK84_003699"
     CDS             complement(5626..6705)
                     /gene="cobT"
                     /locus_tag="KLK84_003699"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBE2010858.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6717..7460)
                     /gene="cobS"
                     /locus_tag="KLK84_003700"
     CDS             complement(6717..7460)
                     /gene="cobS"
                     /locus_tag="KLK84_003700"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBE2010859.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7457..>7931)
                     /gene="cobU"
                     /locus_tag="KLK84_003701"
     CDS             complement(7457..>7931)
                     /gene="cobU"
                     /locus_tag="KLK84_003701"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBE2010860.1"
                     /translation="SQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDELI
                     HADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQRC
                     PAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         2192 a         2131 c         1715 g         1893 t
ORIGIN      
        1 tacgtatagt ttcccgataa agacgcgaca gctacgtccc gcataaggca tttttctctt
       61 tatctttgta cggtacttca tggaacagaa tttttgacct tgtgaatcgt gatatctgtt
      121 ggggaggggc aatttgctca ctgaagcgtg agactcgatt aagcgcacga aacacagaaa
      181 tcaaaaaacc cggccacttt tttacaaagt ggccgggtaa aaataatttt tattttttaa
      241 ctgtcctgag tctcatagag atgtctcaaa actaaaattt ggctcctctg actggactcg
      301 aaccagtgac atacggatta acagtccgcc gttctaccga ctgaactaca gaggaatcgt
      361 gagaacgagg cgaatattag cgatgcccac ccacaatgtc aaagcctgtt ttttaaattt
      421 gaaatcgttt gctgaaataa tctgcatttt gtcgtttatt ccgacacaac tggctttttt
      481 tcacactttt gcggctcgga tcgagggtat ttccacagcc aacgtccagt aaccattcgc
      541 cagtaaaaca gcacggcccg cacagcccag tcggcaaaca ttcccatcca gacaccaacc
      601 acaccccagc caagcatgat tccctgcaca taaccgacta caacccggca accccacatg
      661 ctcaacatag aaacccacat ggcgtaacgg gcatcacgag cacccttaaa tccagcgggt
      721 agcacccatg aggcggacca aataggcata aataaagcat ttagctaaat cagaatcaca
      781 acgacgtgtt taacctgtgg atcctgggtg taaaacgatg ccataacccc ggcaaaggga
      841 gccgttagcc aggcgatggc cgttaatcca agagtggaca gccagaacac atgccgcaac
      901 tgaatctctg cttgcgctat ctgccctacc cccaaccttc ggcctgtaat gatcgtagaa
      961 gcagaaccga gcgcacttcc gggtaagttg ataagagccg caattgagaa cgcgataaaa
     1021 tttccggcaa taacgctggt ccccatcccg gcaacgaaca tttgggttaa taaccgacca
     1081 ctggtaaata acactgattc gacactcgct ggaataccaa tccccatgac ttcccagata
     1141 atgctaaaat tcagcggttt aaaatagctc tttaacgaaa tccttagcgc aggattaaaa
     1201 ccaatcgcca gcacccacaa aattgcaact gcgccaatat aacgagaaat ggttaaaccc
     1261 aacccagccc cgaccaggag aaaaggccgt aaatcaatat gccgctaata ataatattaa
     1321 gaatattcag gctaccgtta atcagtagcg gtattttcgt attccctgca ccacgaagtg
     1381 ccccgctacc aataagagtg atggcagcgg ctggataact gagtacggtc agctccagat
     1441 aagtcaacgc cagtgcttta acttctgtcg tggcatcacc cgcgacgaaa tcaataattt
     1501 gttcgccaaa atgatgaatt agcgttgcca acagtacggc aaacaacgtc atgatcacca
     1561 atgactgccg cgtcgccacc ctcgctcgtc gtcgatcccg cttaccgaga ctaaatgcta
     1621 caacgacagt agtaccaaga tcgatagctg caaaaaaagc cataatgacc atattgaagc
     1681 tgtccgccaa ccccacgccg gccatcgcat cttttcccag ccaactgacc agaaaagtgc
     1741 tcaggccccc catcaacagg acacaggcgt tctccatgaa gataggaaca gcaagcgggg
     1801 ttatctcgcg ctagaacaac actttgtagc tcttgcgttt agagtgccag cgagtgccgt
     1861 gaacaacctg gcgtaaagca gaggagatat tcaaagccga ccttaattgc agaaagtgaa
     1921 accacatttc aaatgatgag ggagaatcag caaagctgca aagattttcg ccaacaaatt
     1981 gtctgcaaat gcaacaaact gttgatagaa acggcaaaca gttgggaaat ttaaaaatcg
     2041 ggtttgacaa aatatttttc gccgttaaga tgtgcctcaa cagcgattcc tctgtagttc
     2101 agtcggtaga acggcggact gttaatccgt atgtcactgg ttcgagtcca gtcagaggag
     2161 ccaaatttct gaaaagcccg cttttatagc gggctttttg ctatatctga taatcaattt
     2221 cctcttcact gttttccatc acctggcgct tgatatcctc aactgacagt cctgcattac
     2281 aaaattccag aaagcgccaa acatagttac gctgaagttg tcctcgcttc agtcccaacc
     2341 agacagtatt agcatcgaaa agatgccgcg tatccaggcg gattaaattc tcttcctctt
     2401 gttcgccgct ggattgttcg gcaactaatc cgatcccaag cccaagagca acataggttt
     2461 taatgacatc agaatcctgt gcacttaata caatatctgc cggcaaacct ttgcgggtaa
     2521 atgcgtcatc agtgcgtgag cgccccgtaa ttccctgtcg gtaagtaatt aacggccact
     2581 tcgctattga ttccagcgtc aatggtgtaa tttgcgttaa gggatgatcg agtggaacaa
     2641 gcaaactatg gtgccaacaa aaccacggga aggcgacgag ctgcgggtca ttactcaaac
     2701 gctcgctggc gataccaata tcagcttcgc cattttgcaa caacgtcgca atttcctgtg
     2761 gcgaaccctg gattagctcg agccgaacct ccgggaaaag ttcgcgaaaa gctttaatga
     2821 cctctggcaa gctataacgt gcctgagtat gcgtcgttgc aatagtgaga acaccagacg
     2881 tatcgttggt aaacaggtct gcaagccgac gaacattact ggcttcattc agaatacgtt
     2941 ctgcaatgac cagtaatgct ttgcccggtt cagtcatccc cagcagtcgc ttacctcgtc
     3001 gaacaaatat ttcgatgcca aattcatcct ccagttcccg aatatgacgg ctaacgcctg
     3061 actgtgaggt aaaaagcata ttcgcaacct ctgtcaggtt gtaatcctga cgtgcagcct
     3121 cgcggattat ctttagttgt tggaaattca cggtaaactc cgggcagttc agatatcccg
     3181 ttattgttaa agtctaatgc ccggcataac aaataataaa aacccgcatc ttattccatc
     3241 ccgatataac gcttagctca ccaattgcca ctgccttttt tccatcactg gagaactaat
     3301 cactgacatt aacaactctt tcactgcctg tgcctgtggt gacaagtttg ctctggcggg
     3361 taaatttaag gacaaagaga gactcattga aggagtggta atgcgtgaca tccacccatt
     3421 tactgcgcca cataacgaac gcgcagccga ttcaggtaat actgcaacgc ccatgccgct
     3481 ggcaatcgct gcggtaagcg tggcaataga ctcaatttca ccaatgactt ttgccgtgag
     3541 ccgccgtagg gaaaaagcct catcaacacg aagtctaaca gcgctgtaat cactggggag
     3601 aaaaaggttc atttgcgcaa tagcattaac atcaacgttt tgccccgggc aatcttgagt
     3661 tcctaccagg aaaagatctt ctttcagcaa agcctgactg gataccccag ccacagggga
     3721 atgctcataa atcaccgcca tatcgagttg gtgatttatc agtttttcgt taagcactgc
     3781 accactattt tcatgaagat agataacgac ctccgggaat tcagcgcgaa ccgcctgtaa
     3841 taagggcatg gtgatggatg acgcagcggt tcctggcgca aagccaatcg agacttgccc
     3901 cgataatgac tgaccaacgt tatgcaccgc cagttgggcc tgctcacact gacgtaaaat
     3961 ggcccgtgca tgggtataga taatttttcc ggcgtctgtt ggcgtaacgc cccgttttgt
     4021 gcgaatcaaa agctgttgat ttaactcacc ttccagtatg gcaacctgct ggctgagcgc
     4081 tggttgtgcg atatgcaata cttcagcagc ctgggtcagg ctaccaatat ctacaatttt
     4141 tacgaagtat ttcaggcgtc tgaagttcat gttgcctccg gtttttaaga atcggcccaa
     4201 gtgccgccat tacttacaac cagtatgcaa gatgcctgcc agttctattt tggcgttgat
     4261 gcgcaagcta accaactgtc aaataagaga ttatgataga ttcgtcattg gcccttttta
     4321 tcagcagtcg cattcacctg ccctataaaa ggagggtatg caccacgatg gttcattacc
     4381 caataagatt gaaagctcac cactttgttg aaattggcag caaacaaaca aaaaaaatgc
     4441 atttcaccct ttgacatcac catgcactgc cattaatatg cgccccgttc acacgattcc
     4501 tctgtagttc agtcggtaga acggcggact gttaatccgt atgtcactgg ttcgagtcca
     4561 gtcagaggag ccaaattcaa aaaagcctgc tttcgagcag gctttttact ttctaattac
     4621 caacgctctt aaaacatctg tcttgaacca gaactaattt gcacaggcat tcccgatcga
     4681 tgttgcaacg cagcatttgc gcgatttaca tcaacttctt gcccgttgat aaacgcccgc
     4741 aaagatgggg ttaccggcaa tggcactttt cggtcagact catattctgc acgattgcgc
     4801 gacagcggct catgaacttc cagccacttt gaaccatccg gttccgttgt gtatttcact
     4861 ggctgatcaa taatttgtac acgagtgcct acaggaacat tatcaaacag atatttgata
     4921 tcgtcattac gcagacgaat acagccctga cttacacgga gcccaatacc aaaattggca
     4981 ttggtaccgt ggatggcata caacctgcca atataaatcg cgtacagccc catggggtta
     5041 tcgggccccg caggaacaaa tgcgggcaaa ctctcccctc gtttcgcata ttcgcgccgg
     5101 gtgttcggcg ttggcgtcca ggttggtgct tcttgtttac gttcaacggt agtcacccag
     5161 ttacgcgggg tttctcgccc agcctggccg ataccaatag gaaagacttc cactgtatta
     5221 ctgtctggcg gatagtaata aagacgcatt tcagcgacgt taaccacaat ccctttacga
     5281 acagtggcgg gcaaaatcag ttgctgcgga atggtgagtt gtgagccagg cttcggcaaa
     5341 aaaacatcag cgcccggatt cgcttccagc atattactta acccttgccc gtattgtgcg
     5401 gcaaaagtct ccagcggctg agtattgtga tcaggaacag ttacagtaag cgactgcccc
     5461 actaaacggc taccctctgg aggtaatgga taagttaccg ccaggctatt ttggctggca
     5521 aaaagcagag caaatgagca aagaatattt acacgacgca tcatgtccct ttcctaagtc
     5581 gcgaatacta tccgataagt atagctttta tcagactttt cgtttttagc tgttcaaatc
     5641 agaagtcgta ttccccggta gaacaatatt actggcagca agttcgccca tgttgttgta
     5701 tatcgcacaa gcagcttcga tgatggacat cgccagagct gcaccactcc cctcacctaa
     5761 acgcatatcc atattgagat aaggctccag ccccaaatgc gagagcgcta tacgcgcgcc
     5821 tttttctgcc gacaagtgag aaggaatgag atacggtttg attgcaggag acatctggca
     5881 ggctgcgagc gcagcagcat aagaaagaaa tccatccagc aacacgggta aaccgcagga
     5941 agcagcacct aacatcactc cagctattcc gaccaaatca aatccaccca cttttgccag
     6001 gacatcaaca ccatcctgag gatttggttg attcaacgta atcgcccgac gcacaacatc
     6061 aattttatta gccagtttat ctgtcggcag gtttgcgcca atcccaacca cttcttcagg
     6121 atcccggcca gtgattgtgc tgactattgc cgctgccggt gtcgtgtttg ccatccccag
     6181 ttcacctaca ccaaacagcg tgacaccgtt tttcgccagc tcccgcgtat aacatatgac
     6241 gtccaaaagc aacttttcag cctgacggcg actcattgcc ggagctgaag caatattgcc
     6301 gctacctcgt gcgacacgca tgttgataag cccggggata ggctcagcag tatcaatacc
     6361 aacatcaatt acgtggacgt tagcgcccgc ttgtgctgcc agcacacaca cgccggttgt
     6421 tccacgggtc atattttcag cctgtatggc tgtcacttct tttggagaaa tagcgacccc
     6481 ttcctcccag acgccgtgat cggcacacat aaccagtacc gcttttttgc ccacatgcgg
     6541 tatgccattc acccccggca ttcctgccag ttgtatggca agcgcctcca gctttcctag
     6601 gctaccaaca ggtttgaata acccgtcaac atgccgttgt gcacgcgaca tagcggcaga
     6661 attgatggct gggatcgtat tcagtaaatc ggcaattatt tgcatgtcac gtttctttat
     6721 agcagtgcca gcaggaagac cagttcacca agctctatgg ctgctcccag cgtatcgccc
     6781 gtttgcccgc ccagcgtacg tttgagaagc tgaccgagga tgaaaatcgc caccatcgtc
     6841 accaccatag cagccacacc atgcataccc agcaataata cggcagcaaa aatagcggct
     6901 aagccgagtg tgacgcaggt ttgtcgccca tcaattttgc caatgaacac attgccaagc
     6961 ccttcttccc gcgcataacg atgacgatac atcaataatg cggcagtccc gcggctgacc
     7021 gcacatgctg ccgccagcga tgcaaggatc ggttcgccac gcagagccaa ctcgcttaac
     7081 accagaatct ttgccagtac cacaaaaatc aatgccagac cgccgtgggt gcctaaacgg
     7141 ctatcacgca tgatttccaa catgcgatcg cgactacgtg cagaaaatac gccatcacag
     7201 gtatcagcga ggccgtccag atggaattcc ccggtcatca gcgccagcac aagtacgcta
     7261 aacagtgccg ccagtggtgc gccacaccat gcctgcagca ccatgaagac cagcccgcta
     7321 atcgcgccaa gcaataatcc aatcaaagga aaagtaataa taccgcgaga ataatgctcg
     7381 aaatccagtc cctgggacca gcgacgcggt acaggcagac gcgtaataaa tgagagcatc
     7441 gcccaaaata atttgctcat ttgattttta ctccaatacc cgaaaccacc agccatactt
     7501 catttgccgc agcggccaac tgctgattta cccgcccggc aatatcacga aaatgtcgtg
     7561 ccagacgact ctccggcaca atccccattc ccacttcgtt agtcactaat acaacctttg
     7621 cggggcaacg ttggcaggca gcaatcaacg actgaatctc agcattaatc gcctgttcca
     7681 tcgcctgata atcccattca tcagggtctt tatcgccgcc ataatcaaac aacagattag
     7741 tcaccattgt ggtaacgcat tccagcaaca cagcctcatt cgggttaatg tctgcatgaa
     7801 ttaattcatc aagatgttgc cagcgctcca ctgtgcgcca gtgctccggg cggctttgcc
     7861 gatgatgttc tatccgtgca gccatctcat catcaaggat ttgcgaggta gcgatataca
     7921 gaacctgtga a
//
LOCUS       DAEEUE010000145         7918 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.145, whole genome shotgun sequence.
ACCESSION   DAEEUE010000145 DAEEUE010000000
VERSION     DAEEUE010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7918
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.145"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            239..742
                     /gene="dps"
                     /locus_tag="KLK84_003702"
                     /gene_synonym="pexB"
     CDS             239..742
                     /gene="dps"
                     /locus_tag="KLK84_003702"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HBE2010861.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1146..1892
                     /gene="glnH"
                     /locus_tag="KLK84_003703"
     CDS             1146..1892
                     /gene="glnH"
                     /locus_tag="KLK84_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HBE2010862.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            2031..2690
                     /gene="glnP"
                     /locus_tag="KLK84_003704"
     CDS             2031..2690
                     /gene="glnP"
                     /locus_tag="KLK84_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HBE2010863.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2687..3409
                     /gene="glnQ"
                     /locus_tag="KLK84_003705"
     CDS             2687..3409
                     /gene="glnQ"
                     /locus_tag="KLK84_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HBE2010864.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            3670..5894
                     /gene="ybiO"
                     /locus_tag="KLK84_003706"
                     /pseudo
     CDS             3670..5894
                     /gene="ybiO"
                     /locus_tag="KLK84_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(5891..6817)
                     /gene="rlmF"
                     /locus_tag="KLK84_003707"
     CDS             complement(5891..6817)
                     /gene="rlmF"
                     /locus_tag="KLK84_003707"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HBE2010865.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            7093..7353
                     /locus_tag="KLK84_003708"
     CDS             7093..7353
                     /locus_tag="KLK84_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBE2010866.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            7618..>7918
                     /locus_tag="KLK84_003709"
     CDS             7618..>7918
                     /locus_tag="KLK84_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HBE2010867.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT         1935 a         2026 c         1978 g         1979 t
ORIGIN      
        1 accgtattta tatatcgaat tatgcaaaag catatttatt ccgaaaattc ctggcgagca
       61 gataaataag aattgttctt atcaatatat ctaactcatt gaatctttat tagttttgtt
      121 tttcacgctt gttaccacta ttagtgtgat aggaacagcc agaatagcgg aacacatagc
      181 cggtgctata cttagtctcg ttaattactg ggacataaca tcaagaggat atgaaattat
      241 gagtaccgct aaattagtta aatcaaaagc gaccaatctg ctttataccc gcaacgatgt
      301 ctccgacagc gagaaaaaag caacagtaga gttgctgaat cgccaggtta tccagtttat
      361 tgatctttct ttgattacca aacaagcgca ctggaacatg cgcggcgcta acttcattgc
      421 cgtacatgaa atgctggatg gcttccgcac cgcactgatc gatcatctgg ataccatggc
      481 agaacgtgcc gtgcagctgg gcggtgtagc tctggggacc actcaagtta tcaacagcaa
      541 aaccccgctg aaaagttacc cgctggacat ccacaacgtt caggatcacc tgaaagaact
      601 ggctgaccgt tacgcaatcg tcgctaatga cgtacgcaaa gcgattggcg aagcgaaaga
      661 tgacgacacc gcagatatcc tgaccgccgc gtctcgcgac ctggataaat tcctgtggtt
      721 tatcgagtct aacatcgaat aaatccatcg ctgatggtgc agaactttag tacccgataa
      781 aagcggcttc ctgacaggag gccgttttgt tttgcagccc acctcaacgc acttatttag
      841 tgcatccatc tgctatcccc agctgattaa gtaaattttt tgtatccaca tcatcacaca
      901 atcgttacat aaagattgtt ttttcatcag gttttacgct aaataatcac tgtgttgagt
      961 gcacaatttt agcgcaccag attggtgccc cagaatggtg catcttcagg gtattgccct
     1021 ataaatcgtg catcacgttt ttgccgcatc tcgaaaaatc aatgagttgc aaaattggca
     1081 cgattttttc atatatgtga atgtcacgca ggggatcgtc ccgtggatag aaaaaaggaa
     1141 atgctatgaa gtctgtatta aaagtttcac tggctgcact gaccctggct tttgcggttt
     1201 cttctcatgc cgcggataaa aaattagttg tcgcgacgga taccgccttc gttccgtttg
     1261 aatttaaaca gggcgataaa tatgtgggct ttgacgttga tctgtgggct gccatcgcta
     1321 aagagctgaa gctggattac gaactgaagc cgatggattt cagtgggatc attccggcac
     1381 tgcaaaccaa aaacgtcgat ctggcgctgg cgggcattac catcaccgac gagcgtaaaa
     1441 aagcgatcga tttctctgac ggctactaca aaagcggcct gttagtgatg gtgaaagcta
     1501 acaataacga tgtgaaaagc gtgaaagatc tcgacgggaa agtggttgct gtgaagagcg
     1561 gtactggctc cgttgattac gcgaaagcaa acatcaaaac taaagatctg cgtcagttcc
     1621 cgaacatcga taacgcctat atggaactgg gcaccaaccg cgcagacgcc gttctgcacg
     1681 atacgccaaa cattctgtac ttcatcaaaa ccgccggtaa cggtcagttc aaagcggtag
     1741 gtgactctct ggaagcgcag caatacggta ttgcgttccc gaaaggtagc gacgagctgc
     1801 gtgacaaagt caacggcgcg ttgaaaaccc tgcgcgagaa cggaacttac aacgaaatct
     1861 acaaaaaatg gttcagtact gaaccgaaat aataacgcta cacctgtaaa gcgcactggc
     1921 agttccctct cccccctggg gagaggatta gggtgagggg cgcaaacccg ctccggggcc
     1981 attaattacc ctgaatttga ttatttacac cacggtaaca ggaacaacat atgcagtttg
     2041 actggagtgc catctggcct gccattccgc ttctgattga aggtgccaaa atgaccctgt
     2101 ggatttcggt cctcggtctg gctggcggtc tggtaatcgg attgctggca ggttttgcac
     2161 gcaccttcgg tggttggata gccaaccacg tcgcgctggt ctttattgaa gtgatccgcg
     2221 gcacacctat cgtcgtccag gtgatgttta tctatttcgc cctgccgatg gcgtttaacg
     2281 acttacgcat cgacccattt actgcggcgg tggtcaccat catgatcaac tccggcgcgt
     2341 acattgcgga aatcacgcgt ggtgcggtgc tgtctatcca caaaggtttt cgtgaagcag
     2401 gactggcgct cggtctttca cgttgggaaa ccattcgcta cgtcatttta ccgctggcac
     2461 tgcgtcgtat gctgccgccg ctgggtaacc agtggatcat cagcattaaa gacacctcgc
     2521 tgtttattgt tatcggcgtg gcggaactga cccgtcaggg gcaagaaatt attgccggta
     2581 acttccgcgc ccttgagatc tggagcgccg tggcggtgtt ctatctgatt attaccctgg
     2641 tgctgagctt tattctgcgt cgtctggaaa gaaggatgaa aatcctgtga ttgaatttaa
     2701 aaacgtctcc aagcactttg gcccaaccca ggtgctgcac aatatcgatt tgaacattgc
     2761 ccagggcgaa gtcgtggtga ttatcgggcc gtccggttcc ggtaaatcga ccctgctgcg
     2821 ctgcatcaac aaactggaag aaatcacctc cggcgatctg attgtcgatg gcctgaaggt
     2881 caacgatccg aaagttgacg agcgcctgat tcgccaggaa gcaggtatgg tgttccagca
     2941 gttttacctc ttcccgcatc tgacggcgct gggaaacgtc atgtttggtc cgctacgcgt
     3001 gcgtggcgcg aacaaagaag aggcggaaaa actggcgcgt gagctgctgg cgaaagtcgg
     3061 tctggcagaa cgtgcacatc actacccttc cgaactttct ggtggtcaac agcagcgtgt
     3121 ggcgattgcc cgcgcgctgg cggtgaagcc gaaaatgatg ctgtttgatg aaccgacttc
     3181 cgctcttgac ccggaactgc gccatgaagt gctgaaggtt atgcaggatc tggctgaaga
     3241 agggatgacg atggtgatcg tgacccacga aatcggtttt gccgagaaag tagcttcgcg
     3301 gctgatcttt atcgacaaag gccggattgc ggaagatggc aatccgcagg tgttgatcaa
     3361 gaacccgccg agccagcgct tgcaggaatt tttgcagcac gtctcttaat aagacacatt
     3421 gcctgatgcg ctacgcttat caggcctaca ggatatctgg caacttatta aaattgcatg
     3481 aacttgtagg acggataagg cgttcacgcc gcatccggca aaaaagcccg cacgttgtca
     3541 gcaacctgct taatatccct tcctcccttt cacccgaaag ggaggcacac cagattcctc
     3601 tcatttaaaa tcgcccctcc cccagcatct atacttatct ttttgctctg ttttctcact
     3661 ggaggagtca tgcggtggat cctgttcatc ctcttctgcc tgctgggcgc acctgcccac
     3721 gcggtatcca tacccggcgt tacaaccaca acgacaacgg actcaacgac tgaaccggcc
     3781 ccggaaccgg atatcgaaca aaaaaagcgg cctatggcgc actggcggat gtgctggata
     3841 acgacacctc gcgtaaagag ttgatcgacc agttgcgtac tgtcgccgcg acgccccctg
     3901 cggaaccggt cccgaagatc gtgccgccga cgctggttga agagcaaacc gtgctgcaaa
     3961 aggtcaccga agtcagccgc cattatggtg aagccctttc cgcccgcttc gggcaacttt
     4021 atcgcaatat caccggctcc ccgcataagc catttaatcc acaaaccttc agcaatgcgc
     4081 tgagccattt ttcaatgtta gcggtattag tgtttggttt ttactggctg attcgcctgt
     4141 gcgcactgcc gctatatcgc aaaatgggtc agtgggcgag gcaaaaaaat cgtgagcgca
     4201 gcaactggtt gcagcttccg gcgatgatta ttggggcgtt tattatcgac ctgctgttac
     4261 tggccctgac attatttgtc ggccaggtat taagcgacaa cctgaatgca ggcagtcgca
     4321 ccatcgcttt ccaacaaagt ttgtttctca acgcctttgc cctgattgaa tttttcaaag
     4381 ccgtactacg cctgattttt tgcccaaacg tggcggagct gcgcccgttc acgattcagg
     4441 acgagagcgc ccgttactgg ggtcgtcgcc tgagctggtt aagcagcctg attggctatg
     4501 gcctgattgt ggccgtgccg attatctcta atcaggtgaa tgtacagata ggtgcgctgg
     4561 cgaacgtcat cattatgctg tgcatgaccg tctgggcgtt gtacctgatc ttccgaaata
     4621 aaaaagagat tacccagcat atgctcaact ttgcggagca ttcgctggct tttttcagcc
     4681 tgtttatccg cgcctttgcg ctggtgtggc actggctggc aagcgcctat tttatcatgc
     4741 tgtttttctt ttcgttgttc gatccgggca acagtctgaa atttatgatg ggtgcaacgg
     4801 tgcgcagcct ggcgattatt ggtatcgcag cgtttgtttc cggtatgttt tcccgctggc
     4861 tggcgaaaac catcaccctc tcgccacata ctcagcgtaa ctatccggag ctacaaaaac
     4921 ggctgaatgg ctggctttcg gcggcgctga aaacggcgcg tattctgacc gtctgcgtgg
     4981 cagtaatgtt gctgttgagc gcatggggat tgttcgattt ctggaactgg ctgcaaaacg
     5041 gcgcggggca gaaaaccgta gatatcctga tccgcatcgc actcattctt ttcttctcgg
     5101 cggttggctg gacggtgctc gccagtttga tcgaaaaccg gctggcttcg gatattcatg
     5161 gccgcccgct acccagcgcc cgtacgcgta ccctgctgac gctgttccgt aacgcgctgg
     5221 cggtgattat cagtaccatc accatcatga ttgtgttgtc ggaaatcggc gtcaatatcg
     5281 cgccattgct ggcaggtgcc ggggcattag gtctggctat ctcgtttggt tcgcaaacgc
     5341 tggtaaaaga tattatcacc ggggtattta ttcagtttga aaacggcatg aacactggag
     5401 atttggtgac tatcgggccg ttgaccggca cagtggaacg gatgtcgatt cgctccgtgg
     5461 gcgtgcgcca ggataccggg gcgtatcaca tcattccgtg gtcttcgata accacctttg
     5521 ctaacttcgt ccgcggtatt ggttcggtag tggcaaacta cgatgttgat cgccatgaag
     5581 atgctgataa agccaatcag gcactgaaag atgcggtaac ggaattgatg gaaaacgaag
     5641 aaattcgcgg gctgattatt ggtgaaccga actttgccgg gattgtcggc ttaagcaata
     5701 ccgcgtttac actgcatgtt tcgttcacca cgctgccact caaacagtgg acggtacgct
     5761 ttgccctcga cagccaggtg aaaaaacatt tcgacctggc gggcgttcgc gcgccagtgc
     5821 agacttatca ggtgctgcct gctccgggcg cgaccccggc tgaaccgctg ccgccggggg
     5881 agccaacgct ttaacgttgg cgattaacaa aacggcggcg ctgctcgtcg ttcataaagg
     5941 tccaggcaat aaagcgactc tgcttttgcc cctgggccat ctcttttttt accaccttca
     6001 ccgcgcccac gtcggtcaga gcacgataca acggcggtaa gttttcacca cgagatacca
     6061 gcgaggtaaa ccacatcacc tgcttcgcga agcctttgct ctcttcaatc atctttttga
     6121 taaagttgac ttcaccgcct tcacaccaca actcctgttg ctgaccacca aagttcagtg
     6181 catcgtcttt gttcagcccc aggttacggc gtttacgctc actacctgcc cgcgccgcag
     6241 cggcggaatc gtggaatggc gggttacaca aggtcgcgtc gtattgctcg tttttgtgga
     6301 tgatgccgtt aaaaatcgcc ccactctctt tttgccgacg cagacgaatg gcgcggttaa
     6361 gccccggatt agcactgata atcgcctgcg cactgcttaa cgcctggctg ctggtttcac
     6421 taccggtaaa acgccagcca tattcatgta cgccaatcag cggataaata cagttcgcac
     6481 caacgccgat atccagaatg ctggcattcg ctggaattgt tccgcttgct tcagccagta
     6541 aatcggcaag gtgatgaata taatccgccc ggcccggtac tggtgggcag agaaaaccgt
     6601 cggggatatc ccagttcgct acggcgtaaa aatgggccag caacgcctta ttgagcgcct
     6661 tcaccgccag cggattggca aagtctacgc tttgctcccc tgcgggtgta agcgtgagga
     6721 attgcctgag ttcaggattg acctgacaaa gcgtggcgag atcgtagcgg ctgtgatgac
     6781 ggttgcgcgg atgcaacccc ggtttctggg cggacatagc attctccttt aagcagcggc
     6841 gtaagatacc cgcataacgc gacagggtaa atatctggct gcgtttatca ttccccaaaa
     6901 ccttaacatg gctatggttt atggcaattc atcataaaaa tcagcattat gcatgtcatt
     6961 gatttgtctt attttgtaaa aatatatcgt tcaaaaaata agcatatctc tgtcaatttt
     7021 ttgcgtcatt tgcatcgcgc aggcgaccta cacttatcag gcactacccg ttgtatttat
     7081 gaggatgtca ttatgaaaaa gtgcctcaca ctactgattg ccaccgtcct gagcggaatc
     7141 tctctcacgg cttatgccgc gcaaccgatg agtaacctgg acagcggtca gctacggccc
     7201 gccggtaccg tttcggcaac tggcgcatca aacctaagcg atctggagga taaactggca
     7261 gaaaaagcgc gcgaacaagg cgcgaaaggt tatgtcatta attccgcagg cggaaatgac
     7321 cagatgttag gtactgcaac catctacaaa taaccccacc aacgtaactg cattcgcctc
     7381 tggatgcagt tttcgcctgt tttgtaatca aaaaacatca aatatgaacg caatgtaaat
     7441 aaatgtattt ctttttcgcg caatgggtga tagaaaatcg catcaagtga taatgcttat
     7501 caaaattact atcacttgca cgaacactat cacgggatta acagtggcat cgcatccgca
     7561 gagatgcttt ctcgtggcag tgaaaatttc aacatataag aaaaagtcac ctgcaaaatg
     7621 gaaaacaatc gcaatttccc tgccagacaa tttcattcgc tcacgttctt tgccggtctt
     7681 tgtattggta ttacgcctgt ggcacaggca ctcgccgccg aagggcaagc taacgcggat
     7741 gacacgctgg ttgtcgaagc atcaacgcct tcgctttatg cgccacaaca atctgccgat
     7801 ccgaaattct cgcgtccggt agcggatact acccgcacga tgacggtgat ttctgaacaa
     7861 gtgattaaag atcagggcgc aaccaacctt accgacgcgc tcaaaaacgt ccccggcg
//
LOCUS       DAEEUE010000146         7915 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.146, whole genome shotgun sequence.
ACCESSION   DAEEUE010000146 DAEEUE010000000
VERSION     DAEEUE010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7915
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.146"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..104)
                     /locus_tag="KLK84_003710"
     CDS             complement(<1..104)
                     /locus_tag="KLK84_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE2010868.1"
                     /translation="MSRKTQRYSKEFKAEAVRTVLENQLSISEGASRLS"
     gene            166..1185
                     /gene="ydfJ"
                     /locus_tag="KLK84_003711"
                     /pseudo
     CDS             166..1185
                     /gene="ydfJ"
                     /locus_tag="KLK84_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            1274..2734
                     /locus_tag="KLK84_003712"
     CDS             1274..2734
                     /locus_tag="KLK84_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBE2010869.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2770..2973)
                     /gene="ydfZ"
                     /locus_tag="KLK84_003713"
     CDS             complement(2770..2973)
                     /gene="ydfZ"
                     /locus_tag="KLK84_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HBE2010870.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(3151..3837)
                     /gene="ydfH"
                     /locus_tag="KLK84_003714"
     CDS             complement(3151..3837)
                     /gene="ydfH"
                     /locus_tag="KLK84_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HBE2010871.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3926..4672)
                     /gene="ydfG"
                     /locus_tag="KLK84_003715"
     CDS             complement(3926..4672)
                     /gene="ydfG"
                     /locus_tag="KLK84_003715"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HBE2010872.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4809..6854
                     /gene="dcp"
                     /locus_tag="KLK84_003716"
     CDS             4809..6854
                     /gene="dcp"
                     /locus_tag="KLK84_003716"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HBE2010873.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQLRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6899..7417)
                     /locus_tag="KLK84_003717"
     CDS             complement(6899..7417)
                     /locus_tag="KLK84_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HBE2010874.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7693..>7915
                     /gene="ydeI"
                     /locus_tag="KLK84_003718"
     CDS             7693..>7915
                     /gene="ydeI"
                     /locus_tag="KLK84_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HBE2010875.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHK"
BASE COUNT         1981 a         1852 c         1990 g         2092 t
ORIGIN      
        1 gataatcggg aagcgccttc actgatcgaa agttgatttt caagaaccgt tctgacagct
       61 tcggctttga actctttaga gtaacgttgg gtttttctgc tcattattag ctccttctga
      121 tgccattcta tttcaggaag gagtgtccgt taaactcagg ctacctcacc catcttgctg
      181 gtgacgctgc gtattattca gggattgggt gcaggtgcgg aaatttccgg tgccggtacg
      241 atgctggcgg aatatgcgcc aaaaggtaag cgcggaatta tctcctcatt tgtggctatg
      301 ggaactaact gcggcacctt aagcgcaacg gcaatctggg cctttatgtt cttcattctc
      361 agtaaagagg aactgctggc gtggggatgg cgtataccgt tcctagcgag cgttgtcgtg
      421 atggtctttg ctatctggtt gcgtatgaat ctgaaagaaa gcccggtctt tgagaaggtt
      481 aacgacagca accaaccgac agcaaaacct gcacctgctg gtagcatgtt ccagagcaaa
      541 tccttctggc tggcaacagg gctgcgtttt ggtcaggcgg gtaactccgg tttaattcag
      601 actttccttg caggctattt agtacagacg ttattgttta acaaagcaat tccaacagat
      661 gcattgatga tcagttcgat tctcggcttt atgaccattc cgttccttgg ttggttatcc
      721 gataaaattg gtcgccggat cccgtatatt attatgaata cctctgcgat tgtgctggcg
      781 tggccaatgc tttctatcat cgtagataaa agctatgccc cgagcaccat tatggttgca
      841 ctgattgtga ttcataactg tgcggtgctg ggattatttg ctctggaaaa cattaccatg
      901 gcagaaatgt tcggctgtaa aaaccgcttc acccggatgg ctatttcaaa agaaattggt
      961 ggtcttatcg cttccggttt tggtcctatc ctggcgggta ttttctgcac catgacggaa
     1021 tcctggtatc cgatcgccat tatgatcatg gcatattcag tgattggttt aatctctgcg
     1081 ctgaaaatgc cagaggtgaa agaccgtgat ttaagtgcgc tggaagacgc tgcggaagat
     1141 caaccgcgtg ttgtaagagc tgcgcaacct tccagaagtc tgtaaaccct caatcccttc
     1201 tcttaccgga gaggggattt ttattcatat aacaaaacat atagcttgcc atatttaaat
     1261 ttaaggaatt gttatgggaa ataatttgtt atcagcaaaa gcgacactcc ctgtttatga
     1321 tcgtaataac ctggccccaa gaattgttca tttaggcttt ggtgcatttc accgtgcgca
     1381 tcagggcgtg tatgccgata ttcttgctac agaacatttc agtgactggg gatattatga
     1441 ggtcaactta atcggcggcg aacagcaaat tgccgattta caacagcaag ataatcttta
     1501 taccgttgcg gaaatgtcgg ccgatgcgtg gacggctcgc gtcgttggcg tcgttaaaaa
     1561 agccttgcac gtacagatgg atggcttaga aaccgtgttg gctgcgatgt gtgaaccgca
     1621 aatcgcgatt gtctctctga caatcaccga aaaagggtat ttccactctc cggcgaccgg
     1681 acagttaatg ctcgagcacc cgatggtcgc tgccgacgtg caaaatcccc accagccgaa
     1741 aacagcaaca ggggtgattg ttgaggcgct ggctcgccgt aaagcggcag gacttcccgc
     1801 atttaccgtc atgtcatgtg acaacatgcc agaaaacggt catgttatgc gtgacgttgt
     1861 cacttcctac gcgcaagccg ttgatgtaaa actggcacaa tggatcgaag ataacgtgac
     1921 tttcccatca acaatggtgg accgtattgt gcccgcagtg acagaggata cgctggcgaa
     1981 aatcgaacaa cttaccggtg tgcgcgatcc tgctggcgtt gcctgtgaac ctttccgcca
     2041 gtgggttatc gaagataact ttgtagccgg acgtccggaa tgggaaaaag cgggagccga
     2101 actggttagt gatgtgctgc cttatgaaga gatgaagttg cgcatgctca acggcagtca
     2161 ttcattcctg gcatatctgg gttatcttgc tggatatcag cacattaatg actgtatgga
     2221 agatgaacat tatcgtcatg cggcgtatgg catgatgttg caggaacaag cgccgacgct
     2281 gaaagtgcag ggcgttgatt tgcaagatta cgctaaccga ttaattgcac gctatagcaa
     2341 cccggcgtta cgtcatcgaa cctggcagat tgcgatggat ggcagccaga aattgccaca
     2401 gcggatgttg gattctgttc gctggcatct ggcgcatgac agcaagttcg atctgctggc
     2461 gctgggcgtc gcgggttgga tgcgttatgt cggtggtgtt gatgaacagg gaaatccgat
     2521 agaaatcagt gacccactgt tacctgttat tcagaaggct gtacaaagta gtgccgaagg
     2581 gaaagcgcgc gtccagtcat tgctggcgat taaggcaatc tttggtgatg atttgccaga
     2641 caatagcttg tttactgcaa aagtgacgga agcgtacttg tctttattag cgcatggcgc
     2701 gaaagcgacc gtggcgaaat attccgtgaa gtaaaaaaag caatgccgcc atcggcggca
     2761 ttcacacact tagttcatgc cgagacgaat caggtccaga ggtgccagtt tctcttcaca
     2821 accttcaaca actacggttt ttccgcggcg gatttgctca gcggtcagac cttcagtatc
     2881 aatcgacagg atcttgccag tgtgaccggt gccgctaacc ataacacggc tgccagtggt
     2941 gattgcgtta cggttacgat cgtaggtcgt cataggtgtt ttctcctttc tgatttacag
     3001 tcgaagccca cgtaacgggc gcaatataaa tactcctgtc gagtgaaata ttttttgttt
     3061 ttgatcaagc tcacatcttt tttgatgcat gcatgagccg taaggcgggg ggaaaagcct
     3121 ctcatcccgg atgggatgag agtaaatgaa ttactcttcg ctaaaccagt cgctgttttc
     3181 ctggcggatc tggcgcacgg attcgctgat ttcctgcaaa tgttgcgtca ttgcacgttc
     3241 taccgcatcg ccatcacgtt tttgcagggc agagaaaata tcaagatgct ggcgtaacag
     3301 catttctggt ggagaaacat ggtcgaaact catatagcgc acgcgatcaa cggtcgcttt
     3361 caggttctca atggtatccc acgccagttg acagtcggca atctgcgtca ggagttgatg
     3421 gaagttgtca tcaagttcaa aaaaatcatc cagttgcttg cgctcaatgg caatgcgttg
     3481 ctggtgaaga ttttgttcca gttggtagca ctggctttcg gtaatcatgc tcgccgcccg
     3541 acgcgccacc gcgcactcaa tggcctgacg gataaaactg ccgttgcgca cctgggccat
     3601 ggaaattttg ttgacgtagc tgccacgctg cggacgaatt tgaatcaggc cgttttccgc
     3661 cagtttaata aaggcttcac gaaccggctg gcgtgacaca ttgaaacgaa cagaaacttc
     3721 tttttccgac aacggtgtgc ctggtgcaat caggcaatgg acaatgtcgc gacgaagaat
     3781 acgataaatc tgctgattga caggctgtgt gggattaagt tgcgtttcga cggtcattcg
     3841 ttcttgcttg tgagtgagtt aactgcatga gtctaccact tttttgtgac aagcaataac
     3901 ccggcagtta cgccgggtat aaaaattact gacggtggac attcagtccg gcatagcttt
     3961 gggtaaccgg catcatttcc agggtattga tattgacgtg agcaggcagc gttgacaccc
     4021 accagacggc ttcgctgaca tcttctggcg tcaatgcaac ggtattttga taggtttttt
     4081 ccgctttacc gtcatcgcct ttaaagcgga cattggaaaa ctcggtgcca cccaccagac
     4141 ccggttcgat gtcggtgacg cgcaccgccg taccatgcag atccgtacgc agattcaggc
     4201 taaactgacg aacaaacgct ttcgtcgcac cgtaaacgtt accaccggca tacggccagc
     4261 tgcctgccgt tgagccaatg ttaataatat gaccatgatt acgttcaacc atacccggta
     4321 agacggcgcg cgtcatatat actaggcctt tgttgttggt atcaatcatc gtttcccagt
     4381 cttcaacgct ggctttatgc gcaggctcca tgcccaacgc caggccggca ttatttacca
     4441 ggatatcaat attgctccac tcggcaggga gcgatgccag catctcttca atagcggcgc
     4501 ggttgcgaac gtccagttgg gcgatataca gattatctcc cagttcgtct gttaactcct
     4561 gcaaccgctc ctggcgacgg ccagtggcga taactttatg cccttgttga ataaaacgac
     4621 gagtaatgca ttcaccaaaa cctgccgttg ctccagttac taaaacgatc atctcactgt
     4681 tcctcaacgc ttttgtgtgt caataacata gcacgtgatg cttaagagcg ggtaatactg
     4741 gtttgcaaga ttgcactccg ttatcagtaa gcctacgcta tggaacacct cgcgttcagg
     4801 agaagaaaat gacaacaatg aatcctttcc ttgtgcaaag cacactgccg tatctggctc
     4861 ctcattttga tcaaattgcc aatcatcact atcgcccggc attcgatgag ggaatgcaac
     4921 aaaagcgggc agaaattgct gccatcgcgc ttaacccgca aacgcctgat ttcaacaata
     4981 ctattctggc actggaacaa agcggagaat taattacccg cgttaccagc gtcttttttg
     5041 cgatgactgc ggcgcatacc aatgatgaat tacagcgtct tgatgaacag ttttccgctg
     5101 aactggcgga actggctaat gatatctatc tgaacggtga attattcgcg cgggtagatg
     5161 ctgtctggca gctccgtgaa tccctggggc ttgatagtga atccatccgc ctggtggagg
     5221 ttattcatca acgttttgtc cttgccggag ccaaacttgc acaagctgat aaagcaaaat
     5281 taaaagtact gaatacagaa gctgcgaccc tgaccagcca gtttaaccag cggttacttg
     5341 cagcaaataa atccggcggt ctggttgtga acgatatcgc gcagctggca ggaatgagtg
     5401 agcaagagat tgcgctggcg gcagaggcgg ctcgcgagaa aggtctggat aacaaatggc
     5461 tgattccgct gctgaatacc acccaacaac cggcgcttgc cgaaatgcgc gatcgtgcga
     5521 cgcgtgaaaa actgtttatt gcgggctgga cgcgagcgga aaaaaatgat gccaatgata
     5581 cccgcgctat cattcaacgt ctggtagaga ttcgcgcaca gcaggcgaaa ctgcttggtt
     5641 ttcctcatta tgccgcatgg aaaatcgccg atcagatggc aaaaacgcca gaagcagcac
     5701 tcaactttat gcgggaaatt gttccagcgg cgcgtcaacg tgcgagcgat gaattagcct
     5761 ccatacaggc ggttatcgat aagcagcagg gcgggtttag cgcgcagccg tgggactggg
     5821 cattttatgc ggaacaggta cggcgggaga aatttgatct tgatgaggcg cagctcaagc
     5881 catattttga attaaacacg gtgttaaatg aaggtgtatt ctggaccgcg aatcagctct
     5941 tcggtattaa gtttgtcgaa cgttttgata ttccggtcta ccatcctgac gttcgtgtgt
     6001 gggaaatttt tgatcataat ggcgtgggac tggcgttatt ttacggtgat ttcttcgccc
     6061 gtgattcaaa aagcggcggt gcatggatgg gcaattttgt tgagcaatca acgcttaatg
     6121 aaacgcatcc ggtaatttat aacgtctgca attatcagaa acccgctgcc ggtgagcctg
     6181 cgttgttact ctgggatgat gtaataacct tattccatga atttggtcat acgctgcacg
     6241 gcctttttgc ccgccagcgt tatgccacgc tttccggcac caacacgccg cgtgattttg
     6301 tcgaatttcc gtcgcaaatc aacgaacact gggcaacgca tccgcaggta ttcgctcgct
     6361 acgcccggca ttatcagagc ggggcagcaa tgcctgacga actgcaacag aaaatgcgta
     6421 atgccagcct gttcaacaaa gggtatgaga tgagcgaact gcttagcgcc gcacttctcg
     6481 atatgcgctg gcattgcctg gaagaaaacg aagcaatgca ggatgtcgat gattttgaat
     6541 tgcgggcgct ggtggcggaa aatatggatc ttcctgctat accgccacgc tatcgcagca
     6601 gttatttcgc ccatattttt ggtggcggat atgctgcagg ttattacgct tatctgtgga
     6661 cgcaaatgtt ggccgatgat ggttaccagt ggtttgttga gcagggcgga ttaacgcgtg
     6721 aaaatgggca gcgttttcgc gaggcgatcc tttccagagg taacagcgaa gatctggaac
     6781 gcctgcatcg acaatggcgt ggtaaggctc ctcagattat gccgatgctg caacatcgtg
     6841 gcttgaacat ataaacacca ccaatggctg gcctggagat atcgccaggc cagcctggtt
     6901 atagcagtaa ttgcagcagc tgggcgaggg caaacctcac cgctaaagct aataccgttt
     6961 cgcagtcgcc agcaaaatgc ataaccgcag tgtagttctg gcctttaata tgccacgcga
     7021 accagacggt acccgctggc gtaccatctt caccgccctc cggtccgccg tagccggtaa
     7081 tggcaatact gacatcagca tccgcacgct ctatggcacc tgttgccatt tctgctgcca
     7141 ctttctcact caccgcagaa tatcgttcaa gagactgctg gcttacgctg aggattttca
     7201 tctttgcctg atcggtgaaa gtaacaaagc ctgcaccgta aaatttgggt gtatcttcag
     7261 ctgcacacag ggcgctagcc aacttaccgc cggtgcatga ttcggcggta gttaagcgta
     7321 attgatccgc gataagtctt tgactcaacg cggatgtcag gttttcaata gtgtcggtat
     7381 ctgcgagttg tacaatttta tctttattaa tgttcactgt tttttgtcca cgctattata
     7441 tgtaaaggtg atttgctttt tctgtatgct cgtccaatat aataattttc aaacatataa
     7501 atccgattat tctgaaaaca tgtttaagac tcttcaatct tcttaacttt agtgagtcaa
     7561 attctctgaa tatttgacag ctagtgggct gattttaaat gccttataac aattggcagt
     7621 attcttattt ggcaattata aaaatagcaa ccagacttaa taagtcaaac caaacgcaat
     7681 taaggagtta gtatgaaatt tcagtccatt gtattagcaa gttttcttgt catgccttat
     7741 gcgttggcag atgaccaggg cggtttaaaa caggatgcag cgccaccgcc gcctcatgca
     7801 atagaagatg gctatcgcgg aaccgatgat gcaaaaaaaa tgaccgttga tttcgcaaaa
     7861 aacatgcacg atggcgcttc ggtttcacta cgcggtaatt tgatttctca caaag
//
LOCUS       DAEEUE010000147         7769 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.147, whole genome shotgun sequence.
ACCESSION   DAEEUE010000147 DAEEUE010000000
VERSION     DAEEUE010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7769
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.147"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..306
                     /locus_tag="KLK84_003719"
     CDS             <1..306
                     /locus_tag="KLK84_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBE2010876.1"
                     /translation="LLIARWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMAL
                     SSTLDSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            278..2206
                     /locus_tag="KLK84_003720"
     CDS             278..2206
                     /locus_tag="KLK84_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HBE2010877.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLPFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTRDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TWVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAASRKDATAQP
                     TLEELAAKLSGGVNGYSR"
     gene            2190..2393
                     /locus_tag="KLK84_003721"
     CDS             2190..2393
                     /locus_tag="KLK84_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE2010878.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2390..3983
                     /locus_tag="KLK84_003722"
                     /pseudo
     CDS             2390..3983
                     /locus_tag="KLK84_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3973..5478
                     /locus_tag="KLK84_003723"
     CDS             3973..5478
                     /locus_tag="KLK84_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HBE2010879.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSPGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5515..5862
                     /locus_tag="KLK84_003724"
     CDS             5515..5862
                     /locus_tag="KLK84_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HBE2010880.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5920..6948
                     /locus_tag="KLK84_003725"
     CDS             5920..6948
                     /locus_tag="KLK84_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HBE2010881.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            7000..7386
                     /locus_tag="KLK84_003726"
     CDS             7000..7386
                     /locus_tag="KLK84_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HBE2010882.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7398..>7769
                     /locus_tag="KLK84_003727"
     CDS             7398..>7769
                     /locus_tag="KLK84_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE2010883.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEG"
BASE COUNT         1793 a         1815 c         2411 g         1750 t
ORIGIN      
        1 ctactcattg ccagatggga actgaccgca gaacaggctg tcgcacagca gttaaaaaat
       61 caggtgtcaa aaggaaatct tatcgatacc ggattttgta tttttgccct tagtaagctg
      121 gcgatggctt tatccagtac gcttgactcc attcctttgt ccatgcaacg acagttcccg
      181 gatttaacgc cgcgtcatat tgatcatctg aaaatcctta ttgctaaggg agcgaatcag
      241 tgcgcgcgtg cgggggataa attaccggat ttactcgatg aatatatcag aacaacaact
      301 gagtaatatg atggtggcag tcactatcgc actgcagcca ctggtcaggg tattgccggt
      361 gacggctgta gaatgggccg atcaaaatta ttacctgcct aaagaatcgt catacggtga
      421 gggggaatgg aaaacgctgc cgtttcaggt cgccatcatg aactgcatgg gcaatgacga
      481 gattcgtaca gttaacctga ttaaatctgc ccgtgttggt tatacaaaaa tggtgcttgg
      541 tgtgatcggg tattttattg agcacaaatc ccgcaacact cttctttttc agccgacgga
      601 ctcagctgct gaagatttta tgaagtctca cgtggaagca acaattcgtg atgttccttg
      661 cctgaaaaaa ctttctccct ggctgggccg gaaacaccgg gataatacgc tcacactgaa
      721 gcgtttttcc tctggtgtgg ggttctggtg tttgggtggt gctgctgcca aaaactaccg
      781 tgaaaaatct gtcgatgtgg tctgctatga cgaactctcc tcgtttgaac cggatgtgga
      841 aaaagaaggt tcgccaacac tgcttggcga taaacgtatt gaaggttcgg tatggccaaa
      901 atccatacgc ggttcaacgc caaaaattaa aggctcctgt cagattgaaa aagctgcgaa
      961 tgaatctgcg catttcatgc ggttttatgt cccttgccct cattgcgggg aggagcagta
     1021 tctgaagttt ggcgatgatg cgacgtcgtt tggcctgaaa tgggagaagg gtaaaccgga
     1081 aacggtgtat tacctgtgtg aacataatgg ctgcgtgatc cgtcagtctg aacttgatca
     1141 gaccgggggg cgctggattt gtgacaatac cgggatgtgg acgcgtgacg gtctgacgtt
     1201 tttcagtgct tcgggtaatg aaattccgcc gccgcgctcc atcactttcc atatatggac
     1261 ggcgtacagt ccgttcacca cctgggtaca gatagtctgt gactggctgg atgcactgaa
     1321 agatcccaac ggcgtgaaaa cttttgtgaa caccacgctg ggcgagacct gggaagaggc
     1381 cgtgggcgaa aaactcgatc accaggtact gatggataag gttgtgcctt acacggcaac
     1441 ggtgcctgtc cgggtggttt atctgacggc gggcattgac tcgcagcgaa accgttttga
     1501 gatgtatgtc tgggggtggg ctccgggaga ggaagccttt ctggtggata aaatcatcat
     1561 catgggacgt cctgatgagg aagagacgct gttacgtgtg gatgcggcga tcaataaaaa
     1621 ataccgccat gcagacggta ccgaaatgac catttcccgt gtctgctggg atatcggggg
     1681 gatcgatggc gaaatcgtgt atcagaggtc aaaaaaacac ggtgttttcc gggtactgcc
     1741 ggtaaaaggt gcctctgtct atggcaagcc ggtgatcacc atgccgaaaa cccgcaatca
     1801 gcggggtgtg tatctgtgtg aggtggggac ggataccgca aaagaaattc tctatgcccg
     1861 aatgaaggcc gatcccaccc ctgtgaatga ggccacatcg tatgccatcc gctttcctga
     1921 taatccggag attttttcgc agacagaggc acaacaactg gtggcggagg aactggtgga
     1981 aaagtgggaa aaaggaaaga tgcgtctgtt gtgggataac aaaaagcggc gtaacgaagc
     2041 gctggactgc ctggtgtatg cctatgcggc attacgtgtg tccgtgcaac gctggcaact
     2101 tgatctggct gtgctggcgg catcccgcaa ggatgcaact gcacaaccga cccttgaaga
     2161 actggcagcg aagctgtccg gaggagtgaa tggttacagt cgctgaactg caggcgctgc
     2221 gtcaggcgcg ccttgattta ttaaccggta aacgggttgt gtcggtacag aaagacggtc
     2281 gcaggatcga atatacggca gcgtccctga atgagcttaa ccgtgcgatc aacgatgcgg
     2341 agtcggttct gggtacgacc cgacgccgcc gaccgctggg agtgaggtta tgaaacgaac
     2401 gcctgtcctg attgatgtga acggcgttcc gcttcgtgag agtctcagct acaacggagg
     2461 cggcgcagga tttggcgggc aaatggcaga gtggttacca ccggcgcaga gtgccgatgc
     2521 ggccctgttg cctgcattgc gtctggggaa tgcccgggca gatgatctgg tgcgcaataa
     2581 cggtatcgcg gctaatgcgg tggccctgca taaggatcat atcgtcgggc acatgtttct
     2641 tatcagctac cgtctgaact ggcgttggct ggggatgcgg gagaccacgg caaaaagttt
     2701 tgtcggggag gtggaagcag cctggtcgga atacgcagaa ggaatgtttg gcgagatcga
     2761 cgtggaaggg aaacgcacgt ttacggaatt tatccgtgaa ggggtgggcg tgcatgcctt
     2821 taacggcgaa atctttgtgc agccggtctg ggatacggaa accacgcagt tattccgtac
     2881 gcgttttaaa gccgtgagtc cgaaacgggt ggacacgcca ggacacggta tggggaacca
     2941 ctttctgcgg gccggggttg aggtcgatcg gtatgggcgg gcgattgcgt accatatctg
     3001 tgaggatgat tttccgttct ctggtgccgg acgctgggag cggatcccgc gtgagcttcc
     3061 caccgggcgt ccggccatgc tgcatatttt tgagccggtg gaggacgggc agacccgtgg
     3121 ggccaatcag ttttacagcg taatggagcg gatgaagatg ctggactccc tgcaggccac
     3181 gcagcttcag tcggccattg tgaaggcgat gtatgcagcc acaatcgaaa gtgaacttga
     3241 taccgaaaag gcatttgaat atatcgcggg ggcaccgcaa ggtcagcagg ataatccgct
     3301 tattaatatt ctggagaagt tcacccgctg gtatgacacg aataacgtga cgctgggcgg
     3361 tgttaaaatt ccgcaccttt tttcctggcg atgcgctgaa cctgcagaca gcgcaggatt
     3421 cagacaatgg attttcggcg cttgaacagg cgctgctgcg gtatattgca gccggtcttg
     3481 gcgtttccta cgaacagttg tcccgtgatt actcgaaggt cagttactca agtgcccgtg
     3541 cctccgccaa tgagtcgtgg cgctatttta tggggcggcg aaaatttatt gcggcccggc
     3601 tggccacgca gatgttttcc tgctggctgg aagaggcgct tcttcggggg attattcgtc
     3661 cgccacgggc gcgttttgat ttttaccagg cgcgttcagc ctggtcacgg gcagagtgga
     3721 ttggctccgg aagaatggcc attgacgggc tcaaggaggt ccaggaatcg gtgatgcgca
     3781 ttgaggccgg actgagcacg tatgagaaag aactggcact gatgggcgaa gattatcagg
     3841 acattttccg ccagcaggtc agggaatccg cagagcgcga aaaagccgga ctctcacgtc
     3901 cggtgtggat agcccaggcg tatcagcagc agatagcgga gagtcgcagg ccggaagagg
     3961 agacaacatc acgtgagacg taatctttca catattatcg ctgcggcatt caatgagccg
     4021 ctgcttctgg agcccgccta tgcgcgggtt ttcttttgcg cgctcgggcg tgagatgggc
     4081 gcagcaagcc tttcggtacc gcaacagcag gtacagcttg acgctcccgg gatgctggct
     4141 gaaacggacg agtacatggc cggaggcaaa cgacctgccc gtgtttaccg ggtggtgaac
     4201 ggtattgctg tactgccggt gaccggcacg ctggtacacc ggctgggtgg catgcggcca
     4261 ttttccggaa tgactggcta tgacggcatt gtcgcctgtc ttcagcaggc aatggcggac
     4321 actcaggtac ggggcgtact gctggacatt gacagtcctg gcgggcaggc caccggcgcg
     4381 tttgactgcg ctgacatgat ttaccgtctc cggcagctga agcctgtctg ggcactgtgc
     4441 aatgacacgg cctgttcagc cgccatgtta ctggcatcgg cctgctcccg acggctggtt
     4501 acccagacat cccgtatcgg ctccattggg gtgatgatgg gccatgtcag ctatgccggt
     4561 catctggcac aggccggtgt ggatatcacg ctgatttacg ccggatcgca caaggtggat
     4621 ggcaatcagt ttgaatccct gccagcaaag gttcgccagg acatgcagca gcgcattgat
     4681 gcggcgcgcc ggatgtttgc tgaaaaagtg gcgatgtata ccggtttatc tgtggatgcg
     4741 gttatgggaa cagaggccgc cgtttttgaa ggtcagtccg gtattgaggc cgaactggcg
     4801 gatgaattaa tcaatgcgtc ggacgccatc agcgtgatgg ctgcgacgct gaacacacac
     4861 gatacaggag gcactatgcc gcaattaact gcaacggagg ctgccgcgca ggagaaccag
     4921 cgagtgatgg ggatcctgac gtgtcaggaa gcgaaaggac gtgaacaact ggccacgatg
     4981 ctggcagggc aacagggtat gagcgttgag caggcccggg cgattctggc cgtggcggca
     5041 ccgcaacaat ctggtgtatc tgcgcagagt gaagccgatc gcattatggc atgtgaagag
     5101 gcgaaaggac gtgaacaact ggcggcaacg ctggcggcga tgccggagat gacggtggaa
     5161 aaagcccgtc cgattctggc tgcttcaccg caggcgaatg ccggaccctc acttcgtgat
     5221 cagattatgg ccctggatga ggcaaaaggg gcagaggcgc aggctgaaaa actggcggcc
     5281 tgtccgggga tgaccgtgga gaatgcccgg gctgtactgg cggcagcctc aggcagggca
     5341 gaaccggttt ctgcatccac aaccgccatg tttgaacatt tcatggcgaa ccattcaccg
     5401 gcagcggtac agggtggcgt gccacagacg tcagcagacg gtgatgcgga cgtgaaaatg
     5461 ctcatggcca tgccatgaag tcagtgctga ccatcaatat gaggttttta caatatggta
     5521 acgaaaacca tcactgaaca gcgtgcggaa gtacgtattt ttgccggtaa tgatccggcc
     5581 cataccgcca caggcagcag cgggatttcc tcggcaacac cggctctgac gcccctgatg
     5641 ctggatgagg ataccgggaa actggtggtc tgggacggac agaaggcagg ccatgtggtt
     5701 ggcatactgg tactgccgct tgaaggtaca gagacggtgc tgacgtatta caagtcgggg
     5761 acctttgcga cggaggccat ccactggcct gagagagtgg atacacacaa aaaggcgaat
     5821 gcctttgccg gcagtgccct gagtcacgca gcgctgccgt aacattttat caggtcactg
     5881 cggtagcctg actgatttct gaatgaaagg aactgattta tgggattgtt tacgacccgc
     5941 cagttactcg gttataccga acaaaaagtt aaatttcgtg cgctgtttct ggagctgttt
     6001 ttccgccgta cggtgaattt ccataccgaa gaggtaatgc tggacaaaat taccggaaaa
     6061 acgccggtgg cggcctatgt ctccccggtt gttgaaggaa aggtgttgcg tcaccgtggc
     6121 ggtgaaactc gtgtactgcg cccgggctac gttaagccga aacacgaatt taattaccag
     6181 caggcggtgg agcgtcttcc cggtgaagat ccggcccagc tgaacgaccc ggcttaccgt
     6241 cgcctgcgta tcatcaccga taacctcaaa caggaagagc acgccattgt ccaggtggaa
     6301 gagatgcagg cggtgaatgc cgtgctgtat ggcaaataca ccatggaagg agagcagttt
     6361 gaaactgttg aggtcgattt tggccggtcg gtgggaaata acattacgca ggcaggaggg
     6421 acggagtgga gcgctcagga ccgcgatacc tttgatccga cgcatgatct ggatgcgtac
     6481 tgtgattttg cttccggcac catcaatatt gccattatgg atggtaaggt ctggcgtctg
     6541 ctgaatggct ttaagctgtt ccgcgaaaaa ctggataccc gtcgtggctc aaattcacag
     6601 ctggaaacgg cagtgaaaga cctgggggcg gtggtgtcct tcaaagggta ttatggtgat
     6661 ctggcaatcg tggtggcaaa aacgtcctat gtggcagagg acggtaccga aaaacgttat
     6721 ctgccggacg gcacactggt actgggaaat acggccgcag agggtattcg ttgctacggt
     6781 gccattcagg atgcgcaggc attgtctgaa ggcgtggtgg cgtcttcccg ttatccgaaa
     6841 cactggctga ccgtgggtga tccggcgcgt gaattcacca tgacgcagtc cgcgccgctg
     6901 atggtgctgc cggatccgga tgagtttgtg gtggtgcagg tgaaataatc cgtgagcggg
     6961 agctaaatgc tcccgttttt cattcaatca ggagcgggta tggctacgaa agaagaaaac
     7021 ctgaaacgtc tttgtgagct ggctgagagg ctggggcgtg aaccggatgt ctccggtagt
     7081 gcggcagata ttgcgcagcg agtggcggaa ctggaggaag agcttggaga tgcgggggag
     7141 gtcgcagagc cggatacgta ctcaccatcg aaagatgatc cgaccgcgca taaaggcgag
     7201 tcagttcctg aacagccagg cattgttacc ggagacgaaa cggggcaggt tacggtggtg
     7261 gcactggcga cactgcatac aaaatgtaat ggttcagtga tatttgtttc accgggaacg
     7321 tcgtttcgtg tttctgctga ggtggctgcc agcatggcgg caggtggtct ggcaaaaagg
     7381 cagtaacagg agatgtcgtg gacggtttcg ataatctgtt tgatgctgcg cttgccggtg
     7441 tcaatgaagt cattctccgt gatatgggga tcagcgccgt cattacgtcc ggagaacttg
     7501 aggggacaca cctgacaggg gtttttgatg atcccgaaag tatttctttt gttgccgggg
     7561 ggatccggct tgaggattcc tcaccgtgcc tgtttgtgaa aacggcagat atatcgcagt
     7621 tacgccgtca ggatacgtta actatcgggg atgattcttt ctttgtggat cgtattactc
     7681 cggatgacgg gggatgctgt tatatccggt taaggcgggg cagtcctgcc ccacagaata
     7741 atgccagaat gaggcgatat gatgaaggg
//
LOCUS       DAEEUE010000148         7758 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.148, whole genome shotgun sequence.
ACCESSION   DAEEUE010000148 DAEEUE010000000
VERSION     DAEEUE010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7758
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.148"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(33..869)
                     /locus_tag="KLK84_003728"
                     /pseudo
     CDS             complement(33..869)
                     /locus_tag="KLK84_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
     gene            1051..2475
                     /gene="sbcB"
                     /locus_tag="KLK84_003729"
     CDS             1051..2475
                     /gene="sbcB"
                     /locus_tag="KLK84_003729"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HBE2010884.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(2518..2745)
                     /locus_tag="KLK84_003730"
     CDS             complement(2518..2745)
                     /locus_tag="KLK84_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HBE2010885.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(2759..3817)
                     /gene="yeeE"
                     /locus_tag="KLK84_003731"
     CDS             complement(2759..3817)
                     /gene="yeeE"
                     /locus_tag="KLK84_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HBE2010886.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3996..5354)
                     /gene="plaP"
                     /locus_tag="KLK84_003732"
     CDS             complement(3996..5354)
                     /gene="plaP"
                     /locus_tag="KLK84_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HBE2010887.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(5344..5406)
                     /gene="yoeI"
                     /locus_tag="KLK84_003733"
     CDS             complement(5344..5406)
                     /gene="yoeI"
                     /locus_tag="KLK84_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HBE2010888.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(5567..6550)
                     /locus_tag="KLK84_003734"
     CDS             complement(5567..6550)
                     /locus_tag="KLK84_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE2010889.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(6596..7420)
                     /locus_tag="KLK84_003735"
     CDS             complement(6596..7420)
                     /locus_tag="KLK84_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBE2010890.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(7503..7757)
                     /gene="yoeB"
                     /locus_tag="KLK84_003736"
     CDS             complement(7503..7757)
                     /gene="yoeB"
                     /locus_tag="KLK84_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HBE2010891.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         2075 a         2020 c         1821 g         1842 t
ORIGIN      
        1 attttcacaa gacgtcgctg atggggcgct tacatttgtt cgaagattgg acggcggata
       61 acattaccca tcttgctatg ctctttcggt acggtccata cgttgtccag caggtcaaat
      121 tctgtctttg ttgccagcct aagctctgag agcctcgccc cccacagcat aagcatctga
      181 tgaaggagct tatttgacgt agacgcacgg cttctttcaa tagcaagcca aatcttagcc
      241 agttcgtgat acgacagtac ccgatcccct acctcagcgc gggagccgaa gtcccttggc
      301 tggatgctca taattgcgca actatctatc aactgacgcc gcatgcacca actaattgct
      361 gatcttagtt gacttagcac ctgccttgct cggcgtggat tatctttttc ttcttcggta
      421 agcaggtcaa cccattgctt aaccgtgata gaagatgccg gacgattagg gaaggcgtca
      481 tgcatgcgct tcataaccgt tgatcgataa agtgcctggg tcttttctct gagagttgta
      541 gagacgtagt tgtcgaacca gtagtcgaga cactgggcga ccgtcatgga gttctccacc
      601 ttctcttcaa aataggtgcg tggatccgtt cctgagaaat agagctttcg caagtcagca
      661 gtgatctgtc tggcatcctt caaagacagg gatgggtatc gaccaagccc aagtcgatta
      721 ggcttgccat gccaacgata gcggtactgg aactggatga cccccttcgg tgaaattcgt
      781 acgctgaggc catcggcatc agccacttct tgtgggcccg aatatggttt accataaata
      841 gtacgcagtt ttgtgtcgct tatagccata aataatattt ggtacgctca gaaataatag
      901 tttggcacac atctggtaca caatatcaca tggacaacag cgcacagtaa acaactatat
      961 ttgacgttcg tagacatact caggtgaaaa aagggttgct tttcgagtta taacagacag
     1021 ttaattaact acagtggaca atgctagaca atgacagaca cagataaaca acctaccttc
     1081 ctctttcacg attacgaaac ctttggcacg caccccgcgt tagatcgccc tgcacagttc
     1141 gcagccattc gcaccgatga cgaattcaat gtcatcggcg aacccgaagt cttttactgc
     1201 aagcccgcgg atgactattt accccggcct ggagcagtat taattaccgg tattaccccg
     1261 caggaggcac gggcgaaagg agaaaacgaa gccgcgtttg ccgcccgtat tcactcgctt
     1321 tttaccgtac cgaagacctg tattctgggc tacaacaatg tgcgtttcga cgacgaagtc
     1381 acacgcaacg ttttttatcg taatttctac gatccttacg cctggagctg gcagcatgat
     1441 aactcgcgct gggatttact ggatgttatg cgtgcctgtt atgccctgcg cccggaagga
     1501 ataaactggc ctgaaaatga tgacggtcta ccgagctttc gccttgagca tttaaccaaa
     1561 gcgaatggta ttgaacatag caacgcccac gatgcgatgg ctgatgtgta cgccactatt
     1621 gcgatggcga aactggtaaa aacgcgtcag ccacgcctgt ttgattatct ctttacccat
     1681 cgtaataaac acaaactgat ggcgttgatt gatgttccgc agatgaaacc cctggctcac
     1741 gtttccggaa tgtttggggc atggcgtggc aataccagct gggtggcacc gctggcgtgg
     1801 catcctgaaa atcgcaatgc cgtaattatg gcggatttgg caggagacat ttcgccatta
     1861 ctggagctgg atatcgacac attgcgcgag cgtttatata ccgcaaaagc cgatcttggc
     1921 gataacgccg ccgttccggt taagctggtg catatcaata aatgtccggt actggcccag
     1981 gcgaatacgc tacgcccgga agatgccgac cgactgggaa ttaatcgtca gcattgcctc
     2041 gataacctaa aaattctgcg tgaaaatccg caagtgcgtg aaaaagtggt agcgatattc
     2101 gcggaagccg aaccgtttac accttcagat aatgtggatg cacagcttta taatggcttt
     2161 ttcagtgacg ccgatcgtgc agcaatgaaa attgtgctgg aaaccgagcc gcgtaattta
     2221 ccggcactgg atatcacttt tgttgataaa cgaattgaaa agttgttgtt caattatcgg
     2281 gcacgcaact tcccggggac gctggattat gccgagcagc aacgctggct ggagcaccgc
     2341 caccaggtct tcacgccaga gtttttgcag ggttatgctg atgaattgca gatgctggta
     2401 caacaatatg ccgatgacaa agagaaagtg gcgctgttaa aagcactttg gcagtacgcg
     2461 gaagagatcg tctaatttga agccgccgcc gctggtacgg cggaggacaa tgttgagtca
     2521 ggctttttga acggtgatgc tccacgctgc atcgccaatc tgctgataat cggtgatggc
     2581 atgtccttct tctgccgccc actgtgggat tgcttcagtt gcctgggtgc aatcaaactc
     2641 aattaccaac tcatcgccgc tggccatttc tgccagtgct gcttttgctt ctatgagtgg
     2701 aaacggacag acctgcgtca ccacatccag ctttttaatc gccatcttta caccttactt
     2761 aatttgccgc agcagttgcc agtcgcgcct tacgctgcgg tcgaacatac acaagccagg
     2821 acgcagtcca gactccgaga atcataaata caaggccaat ccagccctgc caggtcatca
     2881 ttgcagtcat aaccagtcca ttaccgatag agcaaccacc tgcaatactg gcaccgaagc
     2941 ccatcagtac accacctaac ccgctacgta atgttgtttg tgcatcagct gcgcgaacgc
     3001 ggaactcacg gctcgctttg gctgcaataa atgcctccac gaagatccct aacaccagga
     3061 aaacgcccca gttaatgtat ttcacgtcac ccgcgaccag aaattgaaga atattggccg
     3121 ttggggaagt gactcctaac ccaaacatgc gtccggttgc ttcactcagg ggccaggcta
     3181 aaagcgcaat caaaccgatg agtacagcag tgacaaaggg atgccagcgt ttttcaaaca
     3241 gaatatgagc gatcccggtt cggcgcggcg gtaaggtcgc gactttgagt tttggcttct
     3301 tcaactcttt catcaccacc cagagcgtta tcaccagcaa aacggtaacc aacggccaca
     3361 cagacaaatt aaaagtctca gcaatagagt tatgttcagt agtgtagtgc tgcaaggttt
     3421 gatttaaacc actggcgtgt ggagaacgca tcaccgcact catcaccata taagtgaaaa
     3481 gcgcgatcca actgccgatc aatccctcac ccgcgcgata ccaggtcccg gtggcacatc
     3541 cacccgccag aacaattccc agcccgaaaa gatacccacc tataacagta ccaagccacg
     3601 ggaacgcccc ggcttcgtaa gtcagtaggc ccgcctgaat taacgcaaaa acccccacgc
     3661 tttgtgccga aattgcaatc agcaaggcgt aaaacatgcg attatttttc gcgatataca
     3721 tatcgcgaaa accaccagtc aggcaaaaac gcccacgctg catgacaaat cccagcagag
     3781 caccacaaat tagcccgctt aatatcattg aaaacataaa ttaattaacc agatgaatgt
     3841 taatggggaa atttttcatg actggtggaa tgcagaccaa taaccaaatt ctctaaatta
     3901 gaacaaatgg ttattaatga ggatttcgat aaaaaagaat aaaaaaaccg gagccaaatg
     3961 ttcattcgac tccggtttat tattagaaaa gatgattatg ctacgtcttc gtactgcgga
     4021 accggattac ggaagctctt ggtgacgcaa gccaggtaaa tcagaccgat agcagcccag
     4081 atcagaccca gaaccattga gctttcttcc aggttaaccc acagcgcacc aacggtcagc
     4141 gcaccacaca tcggcaggaa cagatactgg aagtgatctt tcagcgtctt gttacgcttc
     4201 tcacggatcc agaactgcga gatgaccgac aggttaacaa aggtgaacgc caccagcgca
     4261 ccaaagttaa tcagcgccgt tgccattacc aggtcgaagt tgattgccag cagggcaatc
     4321 gcgccaacca ggatgatgtt catcgccgga gtacgccatt tcgggtgtac ataaccgaag
     4381 aagcttttcg ggaacacgcc gtcacgaccc attacgtaca tcagacgcgc tacgcctgcg
     4441 tgcgccgcca taccggacgc cagtacggta atggtggaga agatcagcgc gccaacctgg
     4501 aacgctttac ccgcaacgta cagcatgatt tcaggctgtg acgcatccgg atctttaaag
     4561 cgagagatat ccgggaagta cagctgcagg aagtaagttg caaagatgaa gatcatgccg
     4621 ccaatcagcg cggtcaggaa aatcgcacgc gggatcacgc gctctgcatc tttggtttct
     4681 tccgacagat tgctgatgcc gtcaaagccg gtaaaggaga agcacaggat tgtcgccccg
     4741 gtaatcatcg ggataacatg cgcatcgcca gaccagaacg gacgcgtgct cgccagcgta
     4801 ccagcgcctt caccttcaaa tacgccataa acaaccatgc ccagaatcac cgcgatcagc
     4861 actacctgca acacgacgat tacagtgttg acgttcgcta cggatttcag actacgcagg
     4921 ttaaaggcgg tcataaaggc caccagcgcc accacaaaca tccacgatgg aatggaaggc
     4981 accagagctt caaaatagat tttcgccagc agaatgttga tcatcggcgc gaacagatag
     5041 tcgagcagag aagaccaacc caccataaag ccgacagtcg ggctaatgga tttctgggcg
     5101 taagtgtatg cagagccagc agaaggatag cggcgaacca gcttcccgta gctcagagcc
     5161 gtaaacagga tcgcaatcaa tgcgaacgca taggctgtcg gcacatgacc atccgtgagg
     5221 cctgaaacga taccaaatgt atcaaacagc gtcatcggct gcatataggc tagacccatc
     5281 attacaaccg gaactaacgt aagcgtttta cgcaattcca cgcgagaggt gtttggagta
     5341 acgttatgcg acatggttat tctcctttac ggtgataacc gtcgcgtaag caaaaaattg
     5401 ccccattttt ttggattcct cagcgacaac aactgtcgat ttttagtaaa tatctatccg
     5461 gtacgaagcc cggcctcttg gtatgaatga ttggtttgaa gcaaaaaata accgacgcta
     5521 attaaacgtc ggtttttaat cattttttga cagcggcgca ttgtgcctaa atggggggga
     5581 aatgacaaga gaatgagagg cttgtcataa taatttttct ttgaatggct gattttccgt
     5641 catcagattt tgcataaaca ccggcgatcg cactatttgc taaaatttca tctcgccacc
     5701 agccgctggc aagccctgcg gtctgttcat tccagccaat ccagacaggt tcaaagagag
     5761 tttgggcgac cactttcttc tcgattaacg cgccactatc gagaaaacgt tgcgccagat
     5821 accgtggtaa atagccgcag ccaaggccgc taatttgcag ctccagtttg gttttaaaat
     5881 cgaaaacggt aatggcttct tgttcgtcga gaagttgtga ggccgtagaa gcgcctgcct
     5941 gcgcagtgtc ccccaccaca atcgcccgat atcgtttaat gatacgacgg tttaacggtt
     6001 cttcttcctg ggctaacgga tgatgaggcg cgaccgcaaa gacttgttca agatcgccca
     6061 accgcgagaa accaaactca ctggaagatg gtggctcatg catcgcgcca acgataatat
     6121 ccgctcgccc ctgagtcagg gcatcccagg aaccaccgag tacgccattg ataaacttca
     6181 ggcgcgtcac gctatgatgt tgataaaaag cttcaatgag aggcgcaagc agagaaaaag
     6241 gaaacgtgtc gtccacgcca atcaccagct cgttttccca gccttcatgc agtttgatcg
     6301 cctgcttttc cagttctcgc acggtatgca gaacttcccg ccctttctct aataacattt
     6361 ttccggtgcg ggtgaattta gcgcggtgtc cgctacgatc aagcaattga atatttaggt
     6421 cgctttccag cttgtgaacg gtgtaactca gagcggacgg cgtcttgtag agtttggctg
     6481 acgccgccgc aaaactgccc tctttttcta tggcatcgag gatcatcaac acatccagca
     6541 gtggtttcat acacgccccc ttgcagtgca tgcggcgtcc tgctggcgtg atcgtttact
     6601 ccaacggcat taccagcgga tcgggatact ggtattcaaa tcccagttca ttacaaatcc
     6661 gactgccatc aataatctta cctttgccgc tgtccagact atttctgaac tgcggaggtt
     6721 gcagccccag taaacgggcc atctgcggat agaaaacatt acgcgcaggg tgagctggcg
     6781 cacatatatt atagatgtgt ccgcctttag gtgcctgtaa caacagagtg atagcgccaa
     6841 taacatcttc taaatggact aaattaacac catgttcacc atcaggggcg gtttttccgg
     6901 caaagaagcg tcccggatga cgtcccggtc cgaccaggcc cgcaagacgc agaatatcga
     6961 ccgaagtacc gggtaaattg tgcagccagt cttcgagttc ttctaatact cgtccactgt
     7021 tggttaccgg attacgcggg gtagtttctt taaccgtgcc ttgcgcgtcg ccatagacag
     7081 atgttgagct ggtaaaaata attcggggaa tacgatgggc cagcgcgcta tccactaact
     7141 cttgtaccgc ttgtaaatag aactcatcgc cggggccgct acgacgtgcc ggaagcgtaa
     7201 tgaccagcgc atcggcatcc ataagggcat ccagatcgtc agaatcgcaa actaactcag
     7261 gctccatgcg aagcagatag ctatcaatgc cactcattcg ggccgcttcg acaccatctt
     7321 gtgtggtttt actcccggtg acttgccagc ctcgcgctga aagtgacatc gccagcggca
     7381 tacctaacca ccctaacccg acaattgcga cctttttcat ccgttatctc ctgatacacc
     7441 ttagatctat aaggctacgc tagcgtatca aaactgacaa ttcattctat gaatgaatct
     7501 gttcaataat gataacgaca tgctgcaatg agcagtgaat catcagtaac cgcgtatacc
     7561 agacggtgct cctctgtaat gcgtcgggac cagaaacctg acaaattatg tttcaggggt
     7621 tctggtttcc ccttaccttc aaatggtgtt ctgcgggtat ctttgataag ttcattgatc
     7681 tttttaacaa ttcgcttatc tgtttcctgc cagtacagat aatcatccca tgattcctca
     7741 gaccagatta gtttcact
//
LOCUS       DAEEUE010000149         7554 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.149, whole genome shotgun sequence.
ACCESSION   DAEEUE010000149 DAEEUE010000000
VERSION     DAEEUE010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7554
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.149"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            1..1158
                     /gene="rbsA"
                     /locus_tag="KLK84_003737"
                     /pseudo
     CDS             1..1158
                     /gene="rbsA"
                     /locus_tag="KLK84_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1163..2128
                     /gene="rbsC"
                     /locus_tag="KLK84_003738"
     CDS             1163..2128
                     /gene="rbsC"
                     /locus_tag="KLK84_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBE2010892.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2153..3043
                     /gene="rbsB"
                     /locus_tag="KLK84_003739"
                     /pseudo
     CDS             2153..3043
                     /gene="rbsB"
                     /locus_tag="KLK84_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3169..4098
                     /gene="rbsK"
                     /locus_tag="KLK84_003740"
     CDS             3169..4098
                     /gene="rbsK"
                     /locus_tag="KLK84_003740"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBE2010893.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4102..5094
                     /gene="rbsR"
                     /locus_tag="KLK84_003741"
     CDS             4102..5094
                     /gene="rbsR"
                     /locus_tag="KLK84_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HBE2010894.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="KLK84_003742"
     CDS             complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="KLK84_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HBE2010895.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLAYALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6510..7202)
                     /locus_tag="KLK84_003743"
     CDS             complement(6510..7202)
                     /locus_tag="KLK84_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBE2010896.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1922 a         1828 c         1987 g         1817 t
ORIGIN      
        1 aaccatgtat gtcgaagcgg ataaattgct ggctaaactt aacctgcgct ttaaaagcga
       61 caagttagtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg ccaaagtgct
      121 gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga ccgataccga
      181 aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccact gtattgtcta
      241 tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg tttttcgtga
      301 tgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc tgattgagat
      361 gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc caggagatat
      421 ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt ttactttacg
      481 caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg aactgatgaa
      541 agtgctctac ggcgcactgc cgcgcaccag cggttacgtc accctggatg ggcatgaagt
      601 cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct ccgaagaccg
      661 taaacgtgac ggtttagtgc tgggcatgtc agtaaaagag aacatgtcgc tgacagcgct
      721 gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc aggctgtgag
      781 tgatttcatt cgtctgttta atgtgaaaac gccgtcgatg gaacaggcaa ttggtctgct
      841 ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac ccccaaagta
      901 ttgatccttg atgagcctac ccgtggcgta gatgtcggcg cgaaaaaaga gatctatcaa
      961 ctgattaacc agttcaaagc cgatggcttg agtatcattc tggtgtcatc ggagatgcca
     1021 gaagtattag gcatgagcga tcgcatcatc gtcatgcatg aagggcatct cagcggggaa
     1081 tttactcgtg agcaggccac ccaggaagtg ttaatggctg tcgctgtggg caagcttaat
     1141 cgcgtgaatc aggagtaaaa aaatgataac ccagactgtc tctggtcgcc gttatttcac
     1201 gaaagcgtgg ctgatggagc agaaatcgct tatcgctctg ctggtgctga tcgcgattgt
     1261 ctcgacgtta agcccgaact ttttcaccat caataactta ttcaatattc tccagcaaac
     1321 ttcggtgaac gccattatgg cggtcgggat gacgctggtg atcctgacgt cgggcataga
     1381 cttgtcggta ggttctctgt tggcgctaac cggcgcagtt gctgcatcta tcgtcggcat
     1441 tgaagtcaat gcgctggtgg ctgtcgctgc tgctctcacg ttaggtgccg caattggtgc
     1501 ggtaaccggg gtgattgtag cgaaaggtcg cgtccaggcg tttatcgcta cgctggttat
     1561 gatgctttta ctgcgcggcg tgactatggt ttataccaac ggtagcccag tgaataccgg
     1621 ctttactgag aacgccgatc tgtttggctg gtttggtatt ggtcgtccgc tgggcgtacc
     1681 gacgccagtc tggatcatgg ggattgtctt cctcgcggcc tggtacatgc tgcatcacac
     1741 gcgtctgggg cgttacatct acgcgctggg cggcaacgaa gcggcaacgc gtctttctgg
     1801 tatcaacgtc aataaaatca aaatcatcgt ctattctctt tgtggtctgc tggcatcgct
     1861 ggccgggatc attgaagtgg cgcgtctctc ctctgcacaa cccacggcgg ggactggcta
     1921 tgagctggat gctattgctg cggtggttct gggcggtacg agtctggcgg gcggaaaagg
     1981 tcgcattgtt gggacgttga tcggcgcatt aattcttggc ttccttaata atggattgaa
     2041 tttgttaggt gtttcctcct attaccagat gatcgtcaaa gcggtggtga ttttgctggc
     2101 ggtgctggta gacaacaaaa agcagtaata acgactacag gacatcttga atatgaacat
     2161 gaaaaaactg gctaccctgg tttccgctgt tgcgctaagc gccaccgtca gtgcgaatgc
     2221 gatggcaaaa gacaccatcg cgctggtggt ctccacgctt aacaacccgt tctttgtatc
     2281 gctgaaagat ggcgcgcaga aagaggcgga taaacttggc tataacctgg tggtgctgga
     2341 ctcccagaac aacccggcga aagagctggc gaacgtgcag gacttaaccg ttcgcggcac
     2401 caaaattctg ctgattaacc cgaccgactc cgacgcagtg ggtaatgctg tgaagatggc
     2461 taactaggcg aacattccgg ttatcactct tgaccgccag gcaacgaaag gtgaagtggt
     2521 gagccacatt gcatctgata acgtactggg cggcaaaatc gccggtgatt acatcgcgaa
     2581 gaaagcgggt gaaggtgcca aagttatcga gctgcaaggc attgccggta catccgcagc
     2641 ccgtgaacgt ggcgaaggct tccagctggc cgttgctgct cacaagttta atgttcttgc
     2701 cagccagcca gcagattttg atcgcaccaa aggtttgaac gtaatgcaga acctgttgac
     2761 cgctcatccg gatgttcagg ctgtattcgc gcagaatgat gaaatggcgc tgggcgcgct
     2821 gcgcgcactg caaactgccg gtaaatcgga tgtgatggtc gtcggatttg acggtacacc
     2881 ggatggcgaa aaagcggtga atgatggcaa actagcagcg actatcgctc agctacccga
     2941 tcagattggc gcgaaaggcg ttgaaaccgc agataaagtg ctgaaaggcg agaaagttca
     3001 ggctaagtat ctggttgatc tgaaactggt tgttaagcag tagttttaat caggttgtat
     3061 gacctgatgg tgacataaat acgtcatcga cagatgaacg tgtaatataa agaaaagcag
     3121 ggcacgcgcc accctaacac ggtggcgcat tttatggaca tcccgaatat gcaaaacgca
     3181 ggcagcctcg ttgttcttgg cagcattaat gctgaccaca ttcttaatct tcaatctttt
     3241 cctactccag gcgaaaccgt aaccggtaac cactatcagg ttgcatttgg cggcaaaggc
     3301 gcgaatcagg ctgtggctgc tgggcgtagc ggtgcgaata tcgcgtttat tgcctgtact
     3361 ggcgatgaca gcattggcga gagcgttcgc cagcagctcg ccactgataa catcgatatt
     3421 actccggtca gcgtgatcaa aggcgaatca acaggtgtgg cgctgatttt tgttaatggc
     3481 gaaggtgaga atgtcatcgg tattcatgcc ggcgctaatg ctgctctttc cccatcgctg
     3541 gtggaagcgc aacgtgagcg tattgccaac gcgtcagcgt tattaatgca gctggaatca
     3601 ccactcgaaa gtgtgatggc agcggcgaaa atcgcccatc aaaataagac tatcgttgcg
     3661 cttaacccgg ctccggctcg cgaacttcct gacgaactgc tggcgctggt ggacattatt
     3721 acgccaaacg aaacggaagc agaaaagctc accggtattc gtgttgaaaa tgatgaagat
     3781 gcagcgaagg cggcgcaggt actgcatgaa aagggtatcc gtactgtact gattacttta
     3841 ggaagtcgtg gtgtatgggc tagcgtgaat ggtgaaggtc agcgcgttcc tggattccgg
     3901 gtgcaggctg tcgataccat tgctgccgga gataccttta acggcgcgtt aatcacggcg
     3961 ttgctggaag aaaaaccatt gccagaggcg attcggtttg cccatgctgc cgctgcgatt
     4021 gccgtaacac gtaaaggcgc acaaccttcc gtaccgtggc gtgaagagat cgacgcattt
     4081 ttagacaggc agaggtgacg cttggctaca atgaaagatg ttgcccgttt ggcgggcgtt
     4141 tctacctcaa cagtttctca cgttatcaat aaagatcgct tcgtcagtga agcgattacc
     4201 gccaaagttg aagcggcgat taaagaactc aattacgcgc catcagctct ggcgcgtagc
     4261 ctcaaactca atcaaacaca taccattggc atgttgatca ctgccagtac caatcctttc
     4321 tattcagaac tggtgcgtgg cgttgaacgc agctgcttcg aacgcggtta tagtctcgtc
     4381 ctttgcaata ccgaaggcga tgaacagcgg atgaatcgca atctggaaac gctgatgcaa
     4441 aaacgcgttg atggcttact gttactgtgt accgaaacgc atcagccttc gcgtgaaatc
     4501 atgcaacgtt atccgacagt gcctaccgtg atgatggact gggctccgtt cgatggcgac
     4561 agcgatctta ttcaggataa ctcgttgctg ggcggagact tagcaacgca atatctgatc
     4621 gataaaggtc atacccgtat cgcctgtatt accggtccgc tggataaaac tccggcgcgc
     4681 ctgcggttgg aaggttatcg ggcggcgatg aaacgtgcgg gtctcaacat tcctgatggc
     4741 tatgaagtca ctggtgattt tgaatttaac ggcgggtttg acgccatgcg ccaactgtta
     4801 tcacatccgc tgcgtcctca ggccgtcttt accggaaatg acgctatggc tgttggcgtt
     4861 taccaggcgt tatatcaggc agagttacag gttccgcagg atatcgcggt gattggctat
     4921 gacgatatcg aactggcaag ctttatgacg ccaccattaa ccactatcca ccaaccgaaa
     4981 gatgaactgg gggagctggc gattgatgta ctcatccatc ggataaccca gccgaccctt
     5041 cagcaacaac gattacaact tactccgatt ctgatggaac gcggttcggc atagatttac
     5101 gctgtctttt gatcaaatta ttaccatcgg ttgttttcag aagcatgaac attgctgctg
     5161 aagcaacagt aataatgccc atcgtgataa acgtatagtg gaattgttcg acagtcgttg
     5221 tgccttccat cccttcataa atgcgaagga cggccgcact tacagcaacg cctaaactaa
     5281 tcgacagttg ctgcgtgacc gccagaacac tgttaccgct gctggcgttg tcatcggtca
     5341 gatcggcaag tgtgatggta ttcatcgcgg taaactgcgt cgacatagcc atccctaata
     5401 taaacaacgg caagatcagc atccatatcg ccattgccgg tgattgcaaa gagaactgag
     5461 cgatcattag cccaataatc accgtgatcc ccactaatgt atggcgatag cccagacgac
     5521 gtaagacttg ggtaaccatc gattttgcaa taatggaacc taacgctgtc ggtgccatca
     5581 tacagccggc aataaacgcc tgataaccaa atcctacctg taacatcaat ggcataagga
     5641 acggtacaca gccggtcccc agacgggttg caatattgcc tacgataccg atcgagaaag
     5701 tgcgggtttt aaataaatct aatgaaatta atgggtttgg cgtacgtcgc gcatggagaa
     5761 tatagagaag cagtaacccg atgctggtga caattaccgt caaggcaatc cagctggcga
     5821 caatcttttc cccgaatagc tctattccgc ttgaaaagag aacaaggctg aggccaaaca
     5881 gcaaaaagcc agtgatatcg aatctgcgtc gtgcggtggt gaaattgggc atatgtttgc
     5941 gcgcgtaaag aaggcccgca atacctatgg ggatattaat taaaaatatc cagtgccagg
     6001 ttgcccaggt gaccagcacg ccgccaagaa cggggcctaa aattggcccc accagacccg
     6061 gcatggcgac aaaattcaat accggaagaa gttcattacg aggataagca cgcagtaagg
     6121 ccggccgagc aacaggcatc atcattgcgt cgcctatccc ttgaataacc cggaagacaa
     6181 ccagctgtgg tagcgaatta gaaagtgcgt aggccagaga acccaacgtg aacagactca
     6241 cggcaagggt aaaaatgcga cgcgtaccga agcgatcggc tagccatccg cttaccggaa
     6301 taagcatcgc caccgtcagc gtataactga tgatggctga ttgcatcgcg agaggagaac
     6361 gattaaggct atgagcgatt gcgggtaagg cggtattcag aatagtggca tcaagtgcct
     6421 gcatgaagaa ggccatcgcc gcgatccacg gcaaacccga catactacgc ttctttttat
     6481 cgctcattca atgtcctgtt atcgggttat cacttatcag gtgagcgtag cagcgcctga
     6541 caagctttaa atgccgcgtc gccatcgctt tggataatcg catcgacaat cgcctggtgc
     6601 agatccagct ttatcactgt gtcgctggta attgacgtga agtaagtgtg ataaaccgaa
     6661 tggaatagcg aggcgaatga ggtcaaaaac ggattggcgc tcatttcata gatatgctca
     6721 tgccaggcca tatcgacttc gatccagcgt tcacggcgaa agttctcttt taatgccgcc
     6781 atttcggcca ttaacgtatt gagatgcgcc ttctgttccg cggtgccaac cgttgctgcc
     6841 agtaggcagg cttgcggctc cagacagata cgcataacca gaaagtgatc gatgacctga
     6901 tgaaagttct cttctgttat ccaccaagta agcaattcct gatcaagaaa attccagttc
     6961 gattgtggca tgacccgagt accaattcgc ggtcgcggta aaaccatccc ttttgccgtt
     7021 aacgttttga ccgcttcgcg taccgctgta cgactcactc caaattgctc gcccagctca
     7081 atctcaccgg gcaaaatggt gccgggttca tattcacctt ttaagatccg ttgcgccagc
     7141 ttctcagcca gaacatacga aaggtttttc tgtgcagcta actgttgtgc gcttaaaggc
     7201 attacttatc ttcctttttc tttttattcc tccttagtat gccaccagga agtgtgatta
     7261 cggttgcaaa aacggcaaat tgcttgtttt atggcacatt aacggggtat tttgctgaaa
     7321 aaatgcgcgg tcagaaaatt attttaaatt tcctcttgtc aggccggaat aactccctat
     7381 aatacgccac cactgacacg gaacaacggc aaatacgccg ccgggtcagc ggggttctcc
     7441 tgagaattcc gacagagaaa gcaaaaataa atgattgact ctgtagcggg aaagcgtatt
     7501 atgcacaccc ccgcgccgct gagaaaaagc gaagcgcact gctctttaac aatt
//
LOCUS       DAEEUE010000150         7184 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.150, whole genome shotgun sequence.
ACCESSION   DAEEUE010000150 DAEEUE010000000
VERSION     DAEEUE010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7184
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.150"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(296..1198)
                     /locus_tag="KLK84_003744"
     CDS             complement(296..1198)
                     /locus_tag="KLK84_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBE2010897.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="KLK84_003745"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="KLK84_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HBE2010898.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="KLK84_003746"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="KLK84_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HBE2010899.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     DLSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="KLK84_003747"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="KLK84_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HBE2010900.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAFIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="KLK84_003748"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="KLK84_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HBE2010901.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="KLK84_003749"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="KLK84_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7090..>7184
                     /locus_tag="KLK84_003750"
     CDS             7090..>7184
                     /locus_tag="KLK84_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HBE2010902.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGAL"
BASE COUNT         1673 a         1811 c         1878 g         1822 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cggagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcggcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatgcccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgacttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc cactctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg ttcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgctctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gcattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgct
//
LOCUS       DAEEUE010000151         6951 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.151, whole genome shotgun sequence.
ACCESSION   DAEEUE010000151 DAEEUE010000000
VERSION     DAEEUE010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6951
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.151"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(225..1067)
                     /gene="purU"
                     /locus_tag="KLK84_003751"
     CDS             complement(225..1067)
                     /gene="purU"
                     /locus_tag="KLK84_003751"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HBE2010903.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(1117..1575)
                     /locus_tag="KLK84_003752"
     CDS             complement(1117..1575)
                     /locus_tag="KLK84_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HBE2010904.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            1688..2593
                     /gene="rssA"
                     /locus_tag="KLK84_003753"
     CDS             1688..2593
                     /gene="rssA"
                     /locus_tag="KLK84_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HBE2010905.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            2685..3698
                     /gene="rssB"
                     /locus_tag="KLK84_003754"
     CDS             2685..3698
                     /gene="rssB"
                     /locus_tag="KLK84_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HBE2010906.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            3900..4808
                     /gene="galU"
                     /locus_tag="KLK84_003755"
     CDS             3900..4808
                     /gene="galU"
                     /locus_tag="KLK84_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBE2010907.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(4952..5365)
                     /gene="hns"
                     /locus_tag="KLK84_003756"
     CDS             complement(4952..5365)
                     /gene="hns"
                     /locus_tag="KLK84_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HBE2010908.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            5970..6587
                     /gene="tdk"
                     /locus_tag="KLK84_003757"
     CDS             5970..6587
                     /gene="tdk"
                     /locus_tag="KLK84_003757"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBE2010909.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
BASE COUNT         1833 a         1524 c         1700 g         1894 t
ORIGIN      
        1 gaaccttcga agtcgatgac ggcagattta cagtctgctc cctttggccg ctcgggaacc
       61 ccaccacggg gtaatgcttt ttactagcct gctcccttat cgggaagcgg ggcgcatcat
      121 atcaaatgac gtgccgctgt aaagtgttac gttgagaaaa atgaactggt tgcgtaattt
      181 tcatccgtaa cggattaaag gtaaccagtt atttttgctg gcgattaaag aataatcgtc
      241 cgattaccgt aaacaaagac gcgttgcgcc agtactttgt atagcgcacg acttaagacg
      301 tttttctcga cgtcacgacc tgcgcgcatc atatcttcag ctgtgtaggt atgatcgaca
      361 tgaataacgt cctgcatgat gattgggcct tcgtccagat tgtcattcac atagtgagcg
      421 gttgcgccaa taatcttcac accacgttca taggcctggt gataaggacg tgcgccgata
      481 aacgcaggca ggaatgaatg gtgaatattg atgattttat tcgggaagcg tgccacaaat
      541 tccggcgtta atacccgcat atacttcgcc agcaccacat agtcaggttg ataagcatca
      601 atggcgtccg ccatcttttg atcgtgctcg ttgcggctta acccttcatg gcttaccagc
      661 tcaaacggaa tatcaaaacg ctcaaccaga gaacgtaaag tatcgtggtt accaataact
      721 gccgcgattt cgacatccag accgccgtaa ttggctttca tcaacaaatc gccaaggcaa
      781 tgcgcttctt tagtgaccag aatcactatc cggcgacgac cggcaggatt cagctcacgc
      841 acggagcctt ctggcaatgc gctatcgaga tccgccagca gggtggaatc attaaaaatc
      901 ccttccagtt ccgtgcgcat aaaaaagcgc ccggtacggt gatcaacaaa ttcattgttc
      961 tgtacgatat ttaactcgtg cttgtagcaa atattggtaa tacgtgcgat cagacctttt
     1021 tggtccggac aaatagtacg cagaacttta cgttggagtg aatgcattgc tggaaaaacc
     1081 ttgttgagag tgtttgctaa accgtaccgt caaccattat tggccgcagc actttttaaa
     1141 ttttttacct gaaccacaag ggcagggatc gttgcgacca aactgcggac gtgtaccgtc
     1201 aatatagtac cattgaccgt tttctttcaa aaatcgcgaa cgttcaatta ttgcacctgt
     1261 tttgccacct tcggtaaatc gagcaacaaa actgacaaat ccgatattgt cggcgtcctg
     1321 ccaacaatgt tcaaatacgg tcaatcccaa ccattcagta tgcgcaaatc cggtcatcaa
     1381 ttcggcacgt aacgccgctg ctccacaaga gggatgccag gtctttatta aataatcagc
     1441 gtcttgcatc acaaaagcgc agtaacgcga acgcatgaga tgttctggat caggtgcaac
     1501 cttttcacca gacacataag ggtggcaaca taggctatac tcgacagcac taccacaggg
     1561 acaaagctga gacacaaata atctccctgg aaacaataac ggcgtattaa ccgcctgagt
     1621 agcactatgt taaccgagca gtagcgatgt ggctacgatt gcattccagg ggaatcttgc
     1681 gggaataatg agaaagataa aaatagggct ggcgctggga tctggcgcgg cgagaggttg
     1741 gtcgcatatt ggcgttatta atgcgctaaa aaaagtgggt attgaaattg atatcgttgc
     1801 aggatgttca attggttcgc tggtgggcgc ggcctatgca tgcgatcgat tatctgcgct
     1861 ggaagattgg gtaacctctt tcagttattg ggacgtttta cgcctgatgg acctctcctg
     1921 gcagcgcggt gggttactgc gcggcgagcg tgtcttcaat caatatcgcg aaataatgcc
     1981 ggaaacagag atcgaaaatt gttcccgtcg ctttgcggct gttgccacca atttaagtac
     2041 ggggcgtgaa ttatggttta ctgaaggcga tctccatctt gctattcgcg catcatgcag
     2101 tattccagga ctcatggcac ccgttgcaca taacggctac tggctggttg atggggcagt
     2161 agttaaccca attcctattt ctcttacgcg tgcattgggg gctgatattg tgatagcggt
     2221 cgacctgcag cacgatgctc atttgatgca acaagatctg ctctccttta atgtcagtga
     2281 agaaaatagc gagaatggtg attctctacc gtggcatgcg cgtctgaaag aaaggttagg
     2341 cagcataacg acacgtcggg ctgtgacagc gccaacggca acagagatta tgaccacgtc
     2401 tatccaggtg ttggagaacc gccttaaaag gaaccgcatg gcaggtgatc cgcccgatat
     2461 tctgattcaa cctgtttgcc cgcaaatatc tacgcttgat ttccatcgcg cgcacgctgc
     2521 cattgcggcc ggacagctgg cagtggaaag gaaaatggac gaacttttgc cgttggtacg
     2581 caccaacatt tgaccagaat ttttatctac acttaagtta attctgacag gcgcaggtgg
     2641 caatagcatg ccactattga gtaaagccag tcaggggaga gaacatgacg cagccattgg
     2701 tcggaaaaca gattctcatt gttgaagatg agcaggtatt tcgctcgctt ctggattcat
     2761 ggttttcctc attgggagcg acaacggtac tggcggctga tggggtggat gcccttgagt
     2821 tgctgggagg tttcactcca gacctgatga tatgtgatat cgcgatgcca cgaatgaacg
     2881 ggcttaaact gctggagcat atacgtaaca gaggcgacca gaccccagtt ctggtgatat
     2941 ctgccactga aaatatggca gatattgcca aagcgttacg tctgggcgtt gaagatgttt
     3001 tgctgaaacc agttaaagat ctgaatcgct tgcgcgagat ggtttttgcc tgtctctatc
     3061 ccagcatgtt taattcgcgc gttgaggaag aggaaaggct ttttcgcgac tgggatgcaa
     3121 tggttgataa ccctgccgca gcggcgaaat tattacagga actacaaccg ccggttcagc
     3181 aggtgatttc ccattgccgg gttaattacc gtcaattggt tgccgcggac aaacccggcc
     3241 tggtgcttga tattgccgca ctttcggaaa acgatctggc attttattgc cttgatgtca
     3301 cccgagctgg acataatggc gtacttgctg ccttgttatt acgcgcattg tttaacggat
     3361 tattacagga acagcttgca caccaaaatc aacggttgcc agagttgggc gcgttattga
     3421 agcaggtaaa ccatttactt cgtcaggcca atctgccggg gcagtttccg ctattagttg
     3481 gctattatca tcgcgaactg aaaaatctca ttctggtttc tgcgggtctg aatgcgacgt
     3541 taaataccgg cgaacaccag gtgcaaatca gtaatggtgt tccgttaggc actttaggta
     3601 acgcttattt gaatcaattg agccagcgat gcgatgcctg gcaatgccaa atatggggaa
     3661 ccggtggtcg actgcgcttg atgttgtctg cagaatgagc aaacgataac gcgggctaaa
     3721 tttgcattac ctgctaatgt cggctggtgg tactatcgtc gccattcgta taagtaattg
     3781 tcttaattat gctatctcgc ctccttttca gaacttagcc ccttcggggt gctgatatac
     3841 tgggatgcga tacagaaata tgaacacgtt caaaacacga acagtccagg agaatttaaa
     3901 tggctgccat taatacgaaa gtcaaaaaag ccgttatccc cgttgcggga ttaggaacca
     3961 ggatgttgcc ggcgacgaaa gccatcccga aagagatgct gccacttgtc gataagccat
     4021 taattcaata cgtcgtgaat gaatgtattg cggctggcat tactgaaatt gtgctggtta
     4081 cacactcatc taaaaactct attgaaaacc actttgatac cagttttgaa ctggaagcaa
     4141 tgctggaaaa acgtgtaaaa cgtcaactgc ttgatgaagt gcagtctatt tgtccaccgc
     4201 acgtgactat tatgcaagtt cgtcagggtc tggcgaaagg cctgggacac gcggtattgt
     4261 gtgctcaccc ggtagtgggt gatgaaccgg tagctgttat tttgcctgat gttattctgg
     4321 atgaatatga atccgatttg tcacaggata acctggcaga gatgatccgc cgctttgatg
     4381 aaacgggtca tagccagatc atggttgaac cggttgctga tgtgaccgca tatggcgttg
     4441 tggattgcaa aggcgttgaa ttagcgccgg gtgaaagcgt accgatggtt ggtgtggtag
     4501 aaaaaccgaa agcggatgtt gcgccgtcta atctcgctat tgtgggtcgt tacgtactta
     4561 gcgcggatat ttggccgttg ctggcaaaaa cccctccggg agctggtgat gaaattcagc
     4621 tcaccgacgc aattgatatg ctgatcgaaa aagaaacggt ggaagcctat catatgaaag
     4681 ggaagagcca tgactgcggt aataaattag gttacatgca ggccttcgtt gaatacggta
     4741 ttcgtcataa cacccttggc acggaattta aagcctggct tgaagaagag atgggcatta
     4801 agaagtaaca tccgtatcgg tgttatccac gaaacggcgt tgagcaatcg acgccgtttt
     4861 tttatagctt attcttatta aattgtctta aaccggacaa taaaaaatcc cgccgctggc
     4921 gggattttaa gcaagtgcaa tctacaaaag attattgctt gatcaggaaa tcgtcgaggg
     4981 atttaccttg ctcatccatt gcttttttga ttacagctgg agtacggcct tggccagtcc
     5041 aggttttagt ttcgccgttt tcgtcaacgt agctatattt tgccggacgc tgagcacgtt
     5101 tagctttggt gccagattta acggcagcaa ggctattcag cagttcgttc gggtcaatac
     5161 cgtcagcgat cagcatttcg cgatattgct gcagcttacg agtgcgctct tcaacttcag
     5221 cagcagccgc gctttcttct tcgcgacgtt cgttaacaac aacttctaat ttttccagca
     5281 tttcttccag cgtttcaagt gtacattctc ttgcctgcgc acgaagagta cggatgttgt
     5341 tcagaatttt aagtgcttcg ctcattgtag taatctcaaa cttatattgg ggtggtttgt
     5401 tgaggtaata atagagcctt aaattcagtt gtgcaatagc caggaatgta aggaattcaa
     5461 aattgttctt tattttgtgc cgccaataaa tatcttttca taaaattagc cagaaaagac
     5521 gcggcatata gccctattta caccgatgat ttcgcagcac gtgaggttaa aacttcctga
     5581 ttcatgtcac attttatggg gagattatcg taggctgacg acctttcagt cttctgtatt
     5641 agttgtgttt acgagaattc cctattaagc gaatgatgaa aagtagaaca gtcgcaataa
     5701 gagcatggac ttagtattgc actatctcct ggaggtcaac agagggctat tacttgcgca
     5761 acaggttaaa gattgtgaat agttaccagc agtcatttac ccgcttataa caagcgaggc
     5821 agttgtaatg atagctcaga aggattatgc aaggcttcgt aagggagaac gcatataccc
     5881 acttctgtgc atactgttga gctgaaaaac tgacgaatta tgataaactc cagccaactt
     5941 tatttcatat cattgagggc ctgtggctga tggcacagct atatttctac tattccgcaa
     6001 tgaatgcggg taagtctaca gcattgttgc aatcttcata caattaccag gaacgcggca
     6061 tgcgcactgt cgtatatacg gcagaaattg atgatcgctt tggtgccggg aaagtcagtt
     6121 cgcgtatagg tttgtcatcg cctgcaaaat tatttaacca aaattcatca ttatttgatg
     6181 agattcgtgc ggaacatgaa cagcaggcaa ttcattgcgt actggttgat gaatgccagt
     6241 ttttaaccag acaacaagta tatgaattat cggaggttgt cgatcaactc gatatacccg
     6301 tactttgtta tggtttacgt accgattttc gaggtgaatt atttattggc agccaatact
     6361 tactggcatg gtccgacaaa ctggttgaat taaaaaccat ctgtttttgt ggccgtaaag
     6421 caagcatggt gctgcgtctt gatcaagcag gcagacctta taacgaaggt gagcaggtgg
     6481 taattggtgg taatgaacga tacgtttctg tatgccgtaa acactataaa gaggcgttac
     6541 aagtcggctc attaacggct attcaggaaa ggcatcgcca cgattaataa gaatttcttt
     6601 actgacaggg tgagcagggc acttttatcc tgtcagttcg ttttacgcac ttcttccggg
     6661 ctatataccc ttctcggcag ttttttaacg ccgctatacg cctcacaggg ctcttaagca
     6721 ccgacgttga cttgtgacct gtaaagtaca atatccctgt gtttaggcgt tatacatcgt
     6781 cgcaaatatg atgaaggcta atgctgtcgg tttatggaaa agttgctttg ggtaaacaaa
     6841 aaaacggccc cagaaggggt aatgccagtc agttaagcaa ctgactggct ctttttcgga
     6901 gctgtggggt atttccaggg cctctccttt accactctcg ggaaggccct t
//
LOCUS       DAEEUE010000152         6850 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.152, whole genome shotgun sequence.
ACCESSION   DAEEUE010000152 DAEEUE010000000
VERSION     DAEEUE010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6850
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.152"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            151..2583
                     /locus_tag="KLK84_003758"
     CDS             151..2583
                     /locus_tag="KLK84_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBE2010910.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2650..4119
                     /locus_tag="KLK84_003759"
     CDS             2650..4119
                     /locus_tag="KLK84_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBE2010911.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4119..5780
                     /locus_tag="KLK84_003760"
     CDS             4119..5780
                     /locus_tag="KLK84_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBE2010912.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6001..6342
                     /gene="symE"
                     /locus_tag="KLK84_003761"
     CDS             6001..6342
                     /gene="symE"
                     /locus_tag="KLK84_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HBE2010913.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6389..6709)
                     /locus_tag="KLK84_003762"
                     /pseudo
     CDS             complement(6389..6709)
                     /locus_tag="KLK84_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2030 a         1627 c         1574 g         1619 t
ORIGIN      
        1 ctccaatgac tagtctaaaa actagtatta agactatcac ttaaataagt gatactggtt
       61 gtctggagat tcagggggcc agtctaatca tagccaggct attgatttta tgtacaatca
      121 gaatcgtcct gataaaagcg agaacccccc atggcggaac tgaacctaag taacctgacg
      181 gaagcagaca tcattaccaa atgcgttatg ccagccattc tcaatgcagg ctgggacaac
      241 acaacgcaaa tcagacagga ggtcaaactc cgggacggta aagtcatcgt acgtggtaaa
      301 gttgcggcac gcagaacggt aaaatctgct gacatcgtgc tgtaccacaa gccaggcatt
      361 cccttagccg tgattgaagc aaaagccaac aaacatgaaa ttggcaaagg gatgcaacag
      421 ggcattgaat atgcgcgcct gctggacgtt ccttttgttt ttgccaccaa cggtgatggc
      481 tttatcttcc gcgatgccac cgcagccgaa ggtgaatgcc ttgaaaagca aataacgctg
      541 gatgacttcc cctcccccgc tgaactctgg caaaaattct gcctctggaa aggatacaca
      601 caagctcagc ttccggtgat tactcaggac tattacgacg atggcagcgg caaatcgcct
      661 cgttattatc aacttcaggc aatcaacaaa accattgaag ccgtctccaa cgggcaaaac
      721 cgcgttctgc tggtcatggc gaccggaaca gggaaaacct ataccgcatt ccagatcatc
      781 tggcgcctgt ggaaatcaaa aaataaaaaa cgcattttgt tccttgccga tcgcaacatt
      841 ctggtcgacc aaaccaaaaa taatgatttc cagccatttg gtacggcaat gaccaaagtc
      901 agcggacgca ccattgatcc cgcttatgaa attcacctcg cgctctatca ggctataact
      961 ggcccggagg aagaccaaaa agcgtttaaa caagtcgcac cagatttctt cgatctgatc
     1021 gtgatcgacg aatgccatcg cggcagcgca tccgaagaca gcgcctggcg agagatcctt
     1081 gattatttca gttccgccac ccaaattggc ttaaccgcca cgccaaaaga gacgcatgaa
     1141 gtctccagca cggattactt cggcgatccg gtttacgtct actcactaaa agaagggatc
     1201 gaagacggct tcctcgctcc ttataaagtt gtccgtgttg atattgatgt cgacctgcaa
     1261 ggctggcgcc caaccaaagg gcaaactgac ttaaacggcg aagtgattga cgatcgtatc
     1321 tataaccaga aagatttcga tcgcacgatg gtgatcgacg aacgcacaga actggttgcc
     1381 agaaccatta ccgactacct caagcgcacc aatccgatgg ataaaaccat cgtcttctgt
     1441 aacgacatcg atcatgcaga acgtatgcgc cgcgccctgg ttaatctcaa cccgcagcag
     1501 gtgaaaaaga acgacaaata cgtcatgaaa atcaccggcg atgatgaaat tggcaaagct
     1561 cagttggata acttcatcaa cccgaaaaaa ccgtacccgg ttatcgcgac cacttcagag
     1621 ctgatgacca ctggtgtgga tgctaaaacc tgcaaactgg tagtactaga ccagaacatc
     1681 cagtcgatga ccaaattcaa gcaaattatc ggtcgtggta cacgcatcga cgaacgttac
     1741 ggcaaactct ggtttaccat cctcgacttt aaaaaagcca ccgaactgtt tgccgatgag
     1801 cgtttcgatg gcattcccga aaaagtcatg gataccacac cagaggatat cgccgatcca
     1861 gaatctgatt ttgaagagaa actcgaagaa atcagcgaac atgacgacga acaggtaaca
     1921 ggcgttgatg aaccgcctgc gccaccatac caggttaccg ataccgatga tgtcggccca
     1981 tttccggaag aagacgagaa gaaaatccgc aagtttcacg tcaacggtgt agcggtgggc
     2041 gttattgcac agcgcgttca gtattacgac gccgacggta aactggttac cgaatccttt
     2101 aaagattaca cccgcaaaac actgctcaaa gaatatgcct cgctggatga ctttacccgc
     2161 aagtggcagg acgccgatcg caaagaagcg atcattcacg agctggagca acaggggatc
     2221 atctgggaag tactggcaga agaagtcggt aaagatctcg acccgttcga catgctttgc
     2281 cacgtagtgt atggtcagcc gccgttaacc cgcaaagagc gcgccgagaa cgtgcacaag
     2341 cggaactact tcacaaaata ctctgaagca gcgcaagccg tgctcgataa tctgctggat
     2401 aaatacgccg atgcgggcgt acaggagatc gaaagtattc aggtgctgaa acttaaacca
     2461 ttcgacagca tgggcacctt accggagatt attaaaaccg gatttggcga ccgtaacggg
     2521 tataatcagg cgctcagcga gctggaaaac gaaatctacc aattaccgcc ccgctctgct
     2581 taacaactct cttttgcgcc ttctgcaacc atcatgttgc agaaggcctt gtttttcatg
     2641 gaatacaaca tgtctatcag ctcagtaatc aaatcattac aagatattat gcgcaaagat
     2701 gccggtgtgg acggcgatgc gcagcgtctc ggtcagctct cctggctgct gtttttgaaa
     2761 atcttcgatg cccaggaaga ggcgctggaa ctggagcagg ataactatca atatccgatc
     2821 ccacagcgtt atttgtggcg cagttgggcc gccaacgcgc agggcattac cggtgattcc
     2881 ctgctggaat tcgttaatga tgatctgttt ccggcgttga aaaacctcac tgcgcctatc
     2941 gataaaaacc cacgcggcta cgtggtgaag caggcgttca gcgatgccta taactatatg
     3001 aaaaacggta cgctactgcg ccaggtgatc aacaagctga acgaaattga ctttaccagc
     3061 gccagcgaac gccatctgtt tggtgatatt tacgaacaga tccttaaaga tctgcaatct
     3121 gcgggcaatg cgggcgaatt ctatactcca cgcgccgtca ctcgctttat ggtggatcgc
     3181 gttgatccga aactcggcga atccattatg gacccggcct gcggtacggg cggttttctt
     3241 gcctgcgcgt tcgatcatgt aaagaacaaa tacgtgaaga gcgtcgccga tcatcagaca
     3301 ctgcaacaac agatccacgg tgttgagaaa aaacagcttc cgcacctgct ggcgaccacc
     3361 aatatgctgc tgcacggcat tgaagtaccg gtacaaattc gtcacgacaa caccctgaat
     3421 aaaccgcttt cctcctggga tgagcaactg gatgtcattg ttaccaaccc gccgtttggt
     3481 ggcacggaag aagacggtat tgagaagaac tttccggcag agatgcaaac ccgcgaaacg
     3541 gcggatctgt ttctgcaact gattgtggaa gtactggcga aaaacggtcg tgcggcggtg
     3601 gtattgccgg atggcacact atttggcgaa ggtgttaaaa ccaaaatcaa aaagctactt
     3661 accgaagagt gcaacctgca taccatcgtg cgtttaccga atggtgtgtt taatccctat
     3721 accggcatta aaaccaacct gttgttcttt accaaaggtc agccaaccaa agagatttgg
     3781 ttctatgaac atccgtatcc ggcgggcgtg aaaaactaca gcaaaaccaa gccgatgaag
     3841 tttgaagagt ttcaggcgga gatcgactgg tggggtaacg aggccgatgg ttttgccagc
     3901 cgcgtcgaga atgagcaagc gtggaaagtc agcattgatg acgtgattgc gcgcaacttc
     3961 aatctggata ttaaaaaccc acatcaggcg gaaaccgtca gccatgatcc ggacgaactg
     4021 ttagcgcagt atgcaaaaca gcaggcggag atccagacgc tgcgtaatca actgcgcgat
     4081 attcttggcg ctgcgctgtc tggcaaggag gttaactaat gagtgtggaa aagctgatcg
     4141 ttgatcatat ggaaacctgg acctcggcgt tgcaaacccg ttccaccgcc gggcgcggca
     4201 gctccggtaa gattgatttg tatggtatta agaaattgcg tgagctgatt ctggaactgg
     4261 cggtgcgcgg taaactggtg ccgcaggatc cgaatgatga accggcgtcg gacctgctga
     4321 agcgtattgc ggcggaaaaa gcagagctgg tgaagcaagg caaaattaaa aagcaaaaac
     4381 cactgccaga aattagtgag gaagagaagc cgtttgaatt gccggaggga tgggagtggg
     4441 tgcacctccc tgatatttat tgctcaattt ctgagtcatc tagaaaaata aaatcctccg
     4501 aaatactacc tgagggaaaa tatcctgtta tcgagcaaag ccaagaattt atttctggtt
     4561 attgcaacaa tgaatgtctg ttaataaagt taaataatcc agttatagtt tttggtgatc
     4621 acacaaggaa tattaaattt attgattttg attttgttgt cggcgctgac ggtgtgaaga
     4681 ttttatcacc tatattaatt tgtgagcgtt tctttttttg gcagttacga tcatttaaat
     4741 tagatgtaag aggatacgcg agacatttta aagtattaaa tagctgctta tttgcattac
     4801 cccccattgc agaacaagag cgaatagttg aaaaagttag ctctttgatg tccctctgcg
     4861 accaactaga acagcaatcc ctgaccagtc tggacgcaca tcagcaactg gttgaaaccc
     4921 tgttgggaac gcttacagac agccaaaaca ccgcggaact ggctgaaaac tgggcgcgta
     4981 ttagcgagca tttcgacacc ctatttacca ccgaagccag cgtggatgcg ttaaaacaga
     5041 ccattctgca actggccgta atgggcaaac ttgtgccgca ggatccgaat gacgaacccg
     5101 cctctgaact gctcaaacgt attgcgcagg aaaaagctca attggtgaaa gaagggaaaa
     5161 taaaaaaaca aaaaccactg ccgccaatta gcgatgagga aaaaccgttt gaacttccgg
     5221 aagggtggga atggtgtcgt attggtaata tagttaatat taaatctgaa ttagtatcac
     5281 caaaagatta tttaaattta tatcaagtag cacctgatat tattgaaaag ggcactggga
     5341 gagtgatatc aaagagaaca gtgaaagaat caggagtcaa agggccaaat agtcgctttt
     5401 ataaaggaca aattgtttat tctaaaatcc gcccgtcatt atccaaagtt tttttagcag
     5461 agtataatgg tctttgcagt gctgatatgt atccattaga ctgttacatt aatccaaatt
     5521 acttattgaa atatatcctt tccattccat ttcttatgca agtcaaaaaa gcagagaaca
     5581 gaataaaaat gccaaaactt aattctgaca gtttttacaa tattattgtt gcgattcccc
     5641 cctataatga acaacaagca atatttgata agataaattc tattgaagca gtatgcaatg
     5701 gtcttatttc ttatattggg atttatcata aaacccaact gcatcttgca gacgcactca
     5761 ctgacgcagc gataaactaa ttccctcgcc ctcacaaaac caataaagcg gctaaatagc
     5821 cgctttattc acatcaccaa aatttatatt tcacgctaat tttcatctta ttttcattaa
     5881 tttttagctg gactttccct gtatttactg atgatgtatc caggtattta gcacggtgcg
     5941 gatgtgcgcc aacacactcg caccgctaat cacaatcact atttatagag aatagtaatc
     6001 atgactgaca cgcattctat tgcacaaccg ttagaagcag aagtctcccc ggcaaataat
     6061 cgtcaattaa ccgtgagtta tgcgagtcgt tacccggatt acagccgtat tcccgccatc
     6121 accctgaaag gtcagtggct ggaagccgcc ggttttgcca ccggcacggc ggtagatgtc
     6181 aaagtgatgg aaggctgtat tgtcctcacc gcccaacccg ccgccgccga agagagcgaa
     6241 ctgatgcagt cactacgcaa ggtgtgtaag ctgtcggcgc gtaaacaaag gcaggtacag
     6301 gaatttattg gggtaattac aggtaaacag aaagtcgcct gatacattga cccggtagcg
     6361 ccagtgctac cgggtattat tcgccgcgtt ataccaacgc gctctccatc tgtttttcaa
     6421 atgcctgacg aattaatccc ataatgtaag gaagttgtgc gaggtcgctg aaaccaattt
     6481 ctacatcgcc attaccccag cgcccaacat ttgtcacatc tttggcaata cctttcggat
     6541 cgaccagttc atgaaactgc atattaagcg acaatcgcag tcggcttttt tgcggcacaa
     6601 catcaacaaa attcgtttcc gctttaaacg caatatatag cttcaatact tcctgcgtga
     6661 tccctgcgtc caggcgcata acttcatcgc gaagatgatc gaataacact gaggtagcct
     6721 gagtttaacg gacactcctt cctgaaatag aatggcatca gaaggagcta ataatgagca
     6781 gaaaaaccca acgttactct aaagagttca aagccgaagc tgtcagaacg gttcttgaaa
     6841 atcaactttc
//
LOCUS       DAEEUE010000153         6829 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.153, whole genome shotgun sequence.
ACCESSION   DAEEUE010000153 DAEEUE010000000
VERSION     DAEEUE010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6829
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.153"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..327
                     /locus_tag="KLK84_003763"
     CDS             <1..327
                     /locus_tag="KLK84_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HBE2010914.1"
                     /translation="NLFPATFSDGYVDIYGYHLPRPLHFGTQGEGMVGVRPEAITLSD
                     RGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDVGEQYYLEIHPYGMF
                     VLADAA"
     gene            complement(436..1368)
                     /gene="mmuM"
                     /locus_tag="KLK84_003764"
     CDS             complement(436..1368)
                     /gene="mmuM"
                     /locus_tag="KLK84_003764"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HBE2010915.1"
                     /translation="MSQNNPLRALLDKQDILLLDGAMATELEARGCNLADSLWSAKVL
                     VENPELIREVHLDYYRAGAQCAITASYQATPAGFAARGLDEAQSKALIGKSVELARKA
                     REAYLAENPQAGTLLVAGSVGPYGAYLADGSEYRGDYHCSVEAFQAFHRPRVEALLDA
                     GADLLACETLPNFSEIEALAKLLTAYPRARAWFSFTLRDSEHLSDGTPLRDVVALLAG
                     YPQVVALGINCIALENTTAALQHLHGLTVLPLVVYPNSGEHYDAVSKTWHHHGEHCAQ
                     LADYLPQWQAAGARLIGGCCRTTPADIAALKARS"
     gene            complement(1355..2758)
                     /gene="mmuP"
                     /locus_tag="KLK84_003765"
     CDS             complement(1355..2758)
                     /gene="mmuP"
                     /locus_tag="KLK84_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methylmethionine permease"
                     /protein_id="HBE2010916.1"
                     /translation="MQTTQQNAPLKRTMKTRHLIMLSLGGVIGTGLFFNTGYIISTTG
                     AAGTLLAYLIGALVVWLVMQCLGELSVAMPETGAFHVYAARYLGPATGYTVAWLYWLT
                     WTVALGSSFTAAGFCMQYWFPQVPVWVWCVVFCAIIFGLNVISTRFFAEGEFWFSLVK
                     VVTIIAFIILGGAAIFGFIPMQDGSPAPGLSNITAEGWFPHGGLPILMTMVAVNFAFS
                     GTELIGIAAGETENPRKVIPVAIRTTIARLIIFFIGTVFVLAALIPMQQAGVEKSPFV
                     LVFEKVGIPYAADIFNFVILTAILSAANSGLYASGRMLWSLSNERTLPACFARVTKNG
                     VPLTALSVSMLGGVLALFSSVVAPDTVFVALSAISGFAVVAVWLSICASHFVFRRRHL
                     QQGKALSELHYRAPWYPLVPVLGFVLCLVACVGLAFDPAQRIALWCGLPFVALCYGAY
                     FLTQPRNAKQEPEHVAE"
     gene            2966..3982
                     /locus_tag="KLK84_003766"
     CDS             2966..3982
                     /locus_tag="KLK84_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS5 family transposase"
                     /protein_id="HBE2010917.1"
                     /translation="MFVIWSHRTGFIMSHQLTFADSEFSSKRRQTRKEIFLSRMEQIL
                     PWQNMVEVIEPFYPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLF
                     ARLSLDSALPDRTTIMNFRHLLEQHQLARQLFKTISRWLAEAGVMMTQGTLVDATIIE
                     APSSTKNKEQQRDPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQL
                     GNLLHGEEQFVSADAGYQGAPQREELAEVDVDWLIAERPGKVRTLKQHPRKNKTAINI
                     EYMKASIRAKVEHPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRADQMIRQW
                     ERSH"
     gene            4210..4527
                     /locus_tag="KLK84_003767"
     CDS             4210..4527
                     /locus_tag="KLK84_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I deoxyribonuclease HsdR"
                     /protein_id="HBE2010918.1"
                     /translation="MPERENYQQVTLQARLVSALETLNPNVPSDTLIEVAKIVATPQT
                     PVLIKNNKLFHQYLIEGVPVEYSVMEQEQWVTKHTHVRLIDFVLWSTKTGHRVRVFPV
                     SVF"
     gene            complement(4477..4890)
                     /locus_tag="KLK84_003768"
                     /pseudo
     CDS             complement(4477..4890)
                     /locus_tag="KLK84_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            complement(4814..5173)
                     /locus_tag="KLK84_003769"
     CDS             complement(4814..5173)
                     /locus_tag="KLK84_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pdcB"
                     /protein_id="HBE2010919.1"
                     /translation="MKAVITPFVQKELGVATFKVDQEVRKLVEAGRKFIMEPVPRELI
                     EHMDDGLVVSEQTMATNEALQPFFNSDELFRRIGGIDSLVAWLRRKEGQVRLGHVVLA
                     GQQAYHESTGNGMGNPW"
     gene            complement(5201..5380)
                     /locus_tag="KLK84_003770"
     CDS             complement(5201..5380)
                     /locus_tag="KLK84_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PdcA protein"
                     /protein_id="HBE2010920.1"
                     /translation="MARKYNKLSREALKMLLDGVSRREVKQYLAGKQIGARTAIAVLC
                     RQEMVVLKQRMPGSR"
     gene            complement(5385..5765)
                     /locus_tag="KLK84_003771"
     CDS             complement(5385..5765)
                     /locus_tag="KLK84_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBE2010921.1"
                     /translation="MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVA
                     YEEITDLFEVSATYLVATARGHIFNDANKRTALNSALLFLRRNGVQVFDSPELADLTV
                     GAATGEISVSSVADTLRRLYGSAE"
     gene            complement(5765..5986)
                     /locus_tag="KLK84_003772"
     CDS             complement(5765..5986)
                     /locus_tag="KLK84_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBE2010922.1"
                     /translation="MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATF
                     EAYKKAALDAEFASLFDTLDSTNKELVNR"
     gene            6169..>6829
                     /locus_tag="KLK84_003773"
     CDS             6169..>6829
                     /locus_tag="KLK84_003773"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005767712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBE2010923.1"
                     /translation="MTSIQQRAELHRQIWQIANDVRGSVDGWDFKQYVLGALFYRFIS
                     ENFSSYIEAGDDSICYAKLDDSVITDDIKDDAIKTKGYFIYPSQLFCNVAAKANTNDR
                     LNADLNSIFVAIESSAYGYPSEADIKGLFADFDTTSNRLGNTVKDKNARLAAVLKGVE
                     GLKLGDFNEHQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTSV
                     NK"
BASE COUNT         1758 a         1916 c         1675 g         1480 t
ORIGIN      
        1 aacctgttcc cggcaacctt cagcgacgga tacgttgata tctacggcta tcatctgccg
       61 cgcccgctgc actttggtac acagggtgaa gggatggtcg gtgtgcgccc ggaagcgatc
      121 acgctcagcg atcgcggcga agagagccag cgctgcgtga tccgccatgt cgcctatatg
      181 gggccgcagt atgaagtgac ggtggaatgg cacgggcagg agatattatt gcaggtcaac
      241 gctacgcgtc tgcaaccgga cgtcggcgag cagtattatc ttgaaatcca tccgtacggc
      301 atgtttgttc tggcggatgc ggcataacgt tttgtggttt tgtaggccgg ataaggcgct
      361 tgtgccgcca tccggcaatg cctgatgcgc tgcgcttatc aggcctacaa tgaacgatgt
      421 agacccgata aaccctcagc ttcgcgcttt taacgcggcg atatccgcag gcgtggtgcg
      481 acagcacccg ccaatcaacc gtgcgccagc ggcctgccac tgcggcagat aatccgccag
      541 ctgcgcgcaa tgttcaccgt gatgatgcca ggttttgctc acggcatcgt aatgctcgcc
      601 cgagttcgga tacaccacca gcggcagcac cgttaaaccg tgtaaatgct gcaacgcagc
      661 ggtggtgttt tccagcgcaa tacagttaat gcctagcgcc accacctgcg gataacccgc
      721 cagcaacgca accacgtcac gcagcggcgt accgtcgctc aggtgttcgc tgtcgcgcag
      781 ggtaaatgag aaccacgccc gcgcacgcgg atatgcggtc aacagcttgg ccaacgcctc
      841 aatctcggaa aaattcggca gggtttcgca ggccagcaga tcggccccgg catccagcaa
      901 ggcttccacg cgcgggcgat gaaacgcctg aaatgcctca acgctacaat gataatcgcc
      961 acggtattca gagccatccg ccagatacgc gccgtaaggc ccgacggatc ccgccaccag
     1021 aagcgttccc gcctgcgggt tctctgccag atacgcttca cgggctttac gcgccagctc
     1081 cacgcttttg ccaatcagcg ctttcgactg cgcttcatcc agacctcgcg cggcgaagcc
     1141 cgccggcgtc gcctgatagc tggcagtgat cgcgcattgc gcccccgccc ggtagtaatc
     1201 aagatgcact tcgcggataa gctccgggtt ttctaccagc actttggctg accacaggct
     1261 gtcggctaag ttacaccctc gcgcttccag ctccgtcgcc atcgcgccat ccagcagcag
     1321 gatatcctgt ttatcaagaa gagcgcgtaa cggattattc tgcgacatgt tctggctcct
     1381 gttttgcgtt tcggggttga gtaaggaaat aagcaccata gcacaacgca acaaacggta
     1441 acccgcacca caacgcaatt ctctgcgctg gatcgaatgc cagcccaaca caggccacca
     1501 ggcacagcac aaaacctaat actggcacca gcggatacca cggcgcgcga taatgtaatt
     1561 cactcaatgc cttaccttgt tgcagatgac ggcgacgaaa aacaaaatgc gaggcgcaga
     1621 tactcagcca caccgctacc accgcaaacc cggagattgc cgacagcgca acaaataccg
     1681 tgtccggggc caccacgctg gaaaacagcg ccagcacacc accgagcata ctgaccgaca
     1741 gcgccgtcag tggcacgccg tttttcgtta ctcgcgcaaa acaggccggt agcgtacgtt
     1801 cattcgacaa cgaccacagc atgcgcccgg aggcatataa cccggagttc gctgcagaaa
     1861 gaatagccgt caggatcacg aagttaaaaa tatcagcggc gtacgggatc cctactttct
     1921 caaataccag cacaaacggg cttttctcca cgcccgcctg ctgcatcggg atcagcgctg
     1981 ccagcacaaa cacggtgccg ataaagaaaa taatcagtcg cgcgatggta gtacgaatcg
     2041 ctaccgggat aactttgcgc gggttttccg tttcaccggc ggcaatgccg ataagctcgg
     2101 tacccgaaaa agcaaagttc actgccacca tagtcatcaa aatcggtaag ccaccgtgcg
     2161 ggaaccagcc ttctgccgtg atattactca gccccggcgc gggcgagcca tcctgcatcg
     2221 gaataaagcc gaaaatcgcc gccccaccga ggatgataaa ggcgatgata gtgaccactt
     2281 tgaccagcga gaaccagaac tccccttcgg caaaaaagcg cgtggagata acattcagac
     2341 caaaaataat cgcgcagaac accacgcacc agacccatac cggcacctgt ggaaaccagt
     2401 actgcataca gaatccagcg gcggtaaagc tcgaacccag cgccacggtc caggtcagcc
     2461 agtaaagcca ggccacggta tacccggtag ccggaccaag atagcgcgcg gcataaacgt
     2521 gaaacgctcc ggtctccggc atcgcgaccg acagctcgcc cagacactgc ataaccagcc
     2581 agaccaccag cgcaccaatc agataggcca gcagcgttcc cgccgctcca gtggtggaaa
     2641 tgatgtaccc ggtattgaag aataatcctg tgccaatcac gccgcccaag gaaagcataa
     2701 tcaggtgacg cgttttcatt gtgcgcttca gtggcgcatt ttgttgtgtt gtttgcatat
     2761 aaccttctaa acgtatggac atctaaacat ccaaagaagg tttgttatac cgcgtttgtt
     2821 aacagaatgc aaagtcaacc ctgcaccaga cgcagatgcc cgcgccgggc gcttaatagc
     2881 gggtggggat taataatcag atacaaaaag ggccgccagc tcataacgcc tccggaaggt
     2941 gcgaacaagt ccctgatatg agatcatgtt tgtcatctgg agccatagaa cagggttcat
     3001 catgagtcat caacttacct tcgccgacag tgaattcagc agtaagcgcc gtcagaccag
     3061 aaaagagatt ttcttgtccc gcatggagca gattctgcca tggcaaaaca tggtggaagt
     3121 catcgagccg ttttacccca aggctggtaa tggccggcga ccttatccgc tggaaaccat
     3181 gctacgcatt cactgcatgc agcattggta caacctgagc gatggcgcga tggaagatgc
     3241 tctgtacgaa atcgcctcca tgcgtctgtt tgcccggtta tccctggata gcgccttgcc
     3301 ggaccgcacc accatcatga atttccgcca cctgctggag cagcatcaac tggcccgcca
     3361 attgttcaag accatcagtc gctggctggc cgaagcaggc gtcatgatga ctcaaggcac
     3421 cttggtcgat gccaccatca ttgaggcacc cagctcgacc aagaacaaag agcagcaacg
     3481 cgatccggag atgcatcaga ccaagaaagg caatcagtgg cactttggca tgaaggccca
     3541 cattggtgtc gatgccaaga gtggcctgac ccacagccta gtcaccaccg cggccaacga
     3601 gcatgacctc aatcagctgg gtaatctgct gcatggagag gagcaatttg tctcagccga
     3661 tgccggctac caaggggcgc cacagcgcga ggagctggcc gaggtggatg tggactggct
     3721 gatcgccgag cgccccggca aggtaagaac cttgaaacag catccacgca agaacaaaac
     3781 ggccatcaac atcgaataca tgaaagccag catccgggcc aaggtggagc acccatttcg
     3841 catcatcaag cgacagttcg gcttcgtgaa agccagatac aaggggttgc tgaaaaacga
     3901 taaccaactg gcgatgttat tcacgctggc caacctgttt cgggcggacc aaatgatacg
     3961 tcagtgggag agatctcact aaaaactggg gataacgcct taaatggcga agaaacggtc
     4021 taaataggct gattcaaggc atttacggga gaaaaaatcg gctcaaacat gaagaaatga
     4081 aatgactgag tcagccgaga agaatttccc cgcttattcg caccttccct agaacaacaa
     4141 tgcctcgatt ggtttaaaga gctaggttac caataccaaa atggctatga catcgcacca
     4201 gatggtgata tgcctgaacg tgaaaactat cagcaagtca cgctacaggc gcgtttagtc
     4261 tctgccttgg aaaccttaaa cccaaatgtg cccagtgaca ccttaatcga agtagcgaaa
     4321 atcgttgcca caccgcaaac gcctgtactg attaaaaaca acaaactctt tcaccaatat
     4381 ttaatcgaag gtgtgcctgt tgaatactcg gtaatggagc aagaacaatg ggtgaccaag
     4441 catactcatg ttcgattaat tgattttgtg ttgtggtcaa ctaaaactgg ccaccgcgtt
     4501 agagtttttc cagtatcggt tttctgattc gtttggtggt aacccaccat tatattcgtg
     4561 cggtcttagt gcgctgtaat atccaacgat atagtccgtt atggcgtgag ctgcatcgct
     4621 gaaacttaca tagcccgtcg ctggcaccca ttcgttcttc agactcctga agaagcgctc
     4681 cattgggctg ttatcccagc agtttccacg ccgactcata ctctgcctga tccggtatcg
     4741 ccacagtaac tgccggaact gcctgctcgt ataatgactg ccttgatcgc tgtggaacat
     4801 caccccgacg ggcttaccac gggtttccca tgccatttcc agtgctttca tggtaagcct
     4861 gctgtccggc gagaacgaca tggcccagcc tgacctgccc ctctttcctg cgcaaccacg
     4921 ctaccagcga gtcaattcca ccaatacggc gaaacagttc atcgctgtta aaaaacggct
     4981 gcaacgcctc atttgttgcc atagtttgct cggaaacaac gaggccgtcg tccatgtgct
     5041 cgattaactc acgcggcacc ggctccataa taaatttacg gccagcctcc accagctttc
     5101 tgacttcctg atccactttg aatgtggcga cgccaagctc tttttgtaca aagggagtaa
     5161 ttacggcttt cacatcacac ctttcatcac tgattgggct ttatctgctg cccggcattc
     5221 tctgtttaag cacaaccatt tcctgacggc ataacacagc aatagcggtc ctggcaccaa
     5281 tttgcttacc agccaggtat tgctttacct cgcggcgact cacgccatca agaagcatct
     5341 ttaacgcttc acgggacaat ttgttgtatt tgcgtgccat taatctactc cgcagaacca
     5401 tacaatctac gtaacgtgtc ggcgacagaa gatacagata tctctccggt cgcagcccct
     5461 acggtaaggt ctgccagttc aggtgaatca aatacctgca ccccgttacg gcgtagaaat
     5521 aacagcgcac tgtttagcgc ggtacgctta ttggcatcat tgaatatatg ccctctcgct
     5581 gtagccacca ggtaggtggc ggagacttcg aaaaggtcgg tgatctcttc gtaggcaact
     5641 ctggcctgaa ctctcccgat aatggcctct gccctgcccg gatcagacat tcccggcagg
     5701 ccgccgtagc ggtttatatt cgcatcatga agcgcaataa gttcttccgg tgatatatgc
     5761 ctcattatcg gttaaccagt tccttgttgg tggagtccag ggtgtcaaac agggatgcaa
     5821 attcagcatc cagcgccgct tttttgtagg cttcgaaagt agccttgctg acaattactg
     5881 ctggctcacg gcctctgcgg gtgatttcaa cctcttcccc ggcctcaaca ttgttgagca
     5941 cttcagaaag gttgccgcgc gcggtacgga agttaatgga ttgcataaac acctcgtgta
     6001 ctcgttatgt gtacacaatt ataaacttca caggcataaa gcaccagcac tttgcggctt
     6061 aacaaacctc taggcaggtc attcgtagcc taatgtccga actgctaaag catccaagtt
     6121 gctgtagaat caccgccaat tacataagcc tgaaataagt ggatgaaaat gacaagtatt
     6181 caacaacgtg cagagcttca tcgtcaaatc tggcaaattg ctaacgatgt caggggttcg
     6241 gtcgatggat gggattttaa gcaatacgtt ctgggcgcac ttttctaccg ttttatcagc
     6301 gaaaattttt ccagttatat tgaagccggt gatgacagta tctgttatgc gaaactggat
     6361 gacagcgtaa ttactgatga cattaaagac gatgccatca aaaccaaagg ctacttcatc
     6421 tacccaagcc agcttttctg caacgtagct gcgaaagcaa ataccaatga cagattgaat
     6481 gcagatttaa acagcatctt cgttgctatc gaaagttctg cttacggtta tccttcagaa
     6541 gctgacatca aaggtttgtt tgctgatttc gataccacca gtaaccgcct gggtaacacc
     6601 gttaaagata aaaatgcccg cctggctgcg gttctgaaag gggttgaagg gttaaaactt
     6661 ggtgacttca acgaacatca gattgacctg ttcggtgatg cctatgagtt cctgatttct
     6721 aactatgcgg caaatgccgg taagtcaggc ggtgagttct ttacaccgca gcacgtctcc
     6781 aagctgattg cacaactggc tatgcacggg cagaccagcg ttaacaaaa
//
LOCUS       DAEEUE010000154         6717 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.154, whole genome shotgun sequence.
ACCESSION   DAEEUE010000154 DAEEUE010000000
VERSION     DAEEUE010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6717
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.154"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(249..845)
                     /gene="fimE"
                     /locus_tag="KLK84_003774"
     CDS             complement(249..845)
                     /gene="fimE"
                     /locus_tag="KLK84_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HBE2010924.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GNRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(1323..1925)
                     /gene="fimB"
                     /locus_tag="KLK84_003775"
                     /pseudo
     CDS             complement(1323..1925)
                     /gene="fimB"
                     /locus_tag="KLK84_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            3379..4096
                     /gene="nanC"
                     /locus_tag="KLK84_003776"
                     /pseudo
     CDS             3379..4096
                     /gene="nanC"
                     /locus_tag="KLK84_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
     gene            4116..5222
                     /gene="nanM"
                     /locus_tag="KLK84_003777"
     CDS             4116..5222
                     /gene="nanM"
                     /locus_tag="KLK84_003777"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HBE2010925.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            5287..6267
                     /locus_tag="KLK84_003778"
     CDS             5287..6267
                     /locus_tag="KLK84_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HBE2010926.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPTCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         2067 a         1372 c         1382 g         1896 t
ORIGIN      
        1 tagtttttgg agagaagaat gaggaagatg cgtcgagcca cagaaacgtt agctttacat
       61 atagcggagg tgatgtgaaa ttaatttaca agagaaataa tttacatatc aaatagttag
      121 atgctttttg tcgtttttta atatttttat gcttgagaaa aaatacgtaa cttatttatg
      181 atatggacag tttggcccca attgtcttgt gtttatttgt tttttaaatt tattatcaat
      241 aagttaaatc aaacctcttc tctttttaat ttttcgttta tgagattatt tctttcccat
      301 aatccggcaa aacgagcagc attactggcg gtataacgca cagtatggcg aatatttcga
      361 ttcccgagat aatcctgaat taaacgggta tctgcaccac gctccgccag ttcataaccg
      421 caagcatgcc ttaacatatg aggatgagtc tgcgttacgg ttccagcttc aataccggca
      481 tcgcgaataa tgcgataggc ctgctggcga gaaagccgac tcccgcggcg agaaataaat
      541 atagcgtcgg tccggtcagc gcctttccag ttagcacgtt cctgggtcca gcgttccacg
      601 gcttcacgct catcaaaacg taacgggtga acggtagaaa atccgttctt cagtcggcga
      661 atatttattc gaccttcatt aaggtcaagg tcctgataat gcagatcaag cagttcacta
      721 atacgcatcc catgccgata tgccaacaga ataagacaat aatctctggc tcccgttgcc
      781 ccgtaacaaa ccgcctgcat catggcctga acttctttac cggtaagata acgacgttta
      841 ctcacaatag tagtactcct aactgagata tatttgaatg aatacctata ggaaacctca
      901 atcggtcaaa attagcccaa tagataaaaa gataaccaca cacgaagtta tgtggctatc
      961 agtcaattac attaaacatc atacaaaaat aaaatatcac tgatgtgaca acatctattt
     1021 tcattgattt gattcaatat aacaatagat tacagaggat taacttccag ttacttaaaa
     1081 aataaagaaa aaagtgtgga aacaggagaa tttaagtgat gtgtaaactt atgaatgatt
     1141 ttgtttattt attttgaaga acataaacca ttagattttg tatgggtatg gttaacttaa
     1201 tgtttattat gataattaag aatcacactt acaataacat caattaataa atggttaatc
     1261 atttttgata tatcgtaaga ataatgtagt ttttaacacc atccctggta tctcaactat
     1321 ctctataaaa cagcatgacg ctgtcgtcct ctggctctat cccagatgcc gtaaaaacgc
     1381 cctgcattgc tggcggtata ccagacagta tgacgaatat tgcgatgccc aagataatcc
     1441 tagataagtc gcgtatctat tcccatattc gccaaagcaa aaccacacga atagcgtaac
     1501 atgtgcggat gaatctccag tgacaacccg gcattaccac cagaagtcga gataatatgg
     1561 taaaactgtt gccgagaaag cggattcccc ttacgtgata aaaataccca ctcgctctca
     1621 gcatgcggat aagaagtacg gatactcaac cagtttttta aagcctgaac ttctttgttc
     1681 aatagcgggt gcgttgttga aaagcctttt tttaatcgat ggatatatat acactttgct
     1741 ttaagatcaa tatccgaaat cctcaatcga caaatttcac tcgcccggaa accatgaata
     1801 aaacaaagca aaatcagaca ataattacgt gttgcatgag gcccggtatt tgctgcttta
     1861 aggagtgact cgatttcact atgggtaagg aagttccttt ttttgttatc agccttattc
     1921 ctcatcgttt tcccttataa ttacagacgc gcactagctg tgctgggtta gcaataatcc
     1981 aacattttta tcctgattat gtttataaaa gcgaacgttt gcttaataac ttacgtaagt
     2041 ggaccagttc ttctgagcga acttaaatgg agtagcgata gttacttata ataactttgc
     2101 catacaaacg ccatgctctt gcatgctatg tacctttata tatttatcaa tctgatacga
     2161 atctaataat aaccctcatc attaatgcaa ctaatcttat ctatatatca tgtgatatgt
     2221 tctgtaacaa gtaaaatcaa cataaaaaat tcagcaaaat ttaatatttg ttaaactagc
     2281 cgtctcaaat atgacattat tagttaactg catttgccac aaaatggcta tttcatggaa
     2341 cgcagtaatt tcttagtggc tattatcatg ctaattctta gatccctagc gattattcct
     2401 gcctggttcg tcagcttagg ccatgagatt gttacattcc tttttatcct ggagatatgg
     2461 gggaacgcag gcgacgcttt ttcatctaca tccgttggta aatatagctt tggtcactac
     2521 gacttaagcc ttcctcaatc acagctattc gccgggcgat acggcaaaca aactaagtga
     2581 aacatcatcc tgaaaacacc aacatcaaca agcctctcca gatcgacatc aggagtgacc
     2641 agttacaagc cacaaaccaa gaaacatatt tcatgatgag aattatgctc agcctgatgg
     2701 cgagtaaacc cttattttca ccactctcac tgagttagct tgcaaagctt tctttgttta
     2761 gattctaaat aaattattta taaaacatat agttaaaaga aaatctttag tttttgaaga
     2821 ccaccgcaag tgttcgtctg gcttcacatg gcatcttctt cttagaaaaa gatcgacata
     2881 ttttgtgacg cgaattgcaa atctggtttt gttgtatgga ttgcgtgatt tttgatctgg
     2941 tataacaggt ataaagatgc accacgatag tcaatgagac tgggcatctc gtaatctatg
     3001 gcaaacatca cttcagttct ttcacatcgg gtgatgaaaa tgcacttcag tctgaaagga
     3061 atatgaaaat gagatcaaca gacactctat tttatgactc tgggtaaaat gggttgagta
     3121 agtgatataa cttacgaata ttcaaatcaa ttaaaaatca gaagagattt tatactcagg
     3181 tatttaatct ggatctctgt ttatttaaat aatgttaaaa gagaattttc acaggagacc
     3241 ttatacaaaa aatataaaat acagttaccc gttgccaaag acactataag cctggcaaaa
     3301 aaatattaca caacataaat gctaattgtt tatgcgggct ttgtattgct ttctgtatcc
     3361 tacaaatgag tgaaatttat gaaaaaggct aaaatacttt ctggcgtatt attactgtgc
     3421 ttttcgtccc cattaatttc tcaggctgcg acactggacg tacgtggtgg atatcgtagt
     3481 ggaagccacg cctatgagac tcgactcaaa gtcagtgagg gatggcaaaa tggatggtgg
     3541 gcaagcatgg aaagtaatac ctggaatacc attcatgata ataaaaagga aaatgccgca
     3601 catcaatgat gttcaggttg aagttaatta cgcaattaaa cttgatgatc aatggacggt
     3661 gcgcccggga atgttaacgc attttagcag caacggcaca cgctacggac cctacgtaaa
     3721 actgtcctgg gacgcgacaa aagatcttaa ttttggcatt cgctatcgtt acgactggaa
     3781 agcttaccga caacaagact tatccggtga tatgtctcgt gataacgttc atcgttggga
     3841 tggatatgtc acttaccata ttaatagtga tttcaccttc gcatggcaaa cgacgctata
     3901 cagcaaacag aatgattatc gctatgcaaa ccacaagaaa tgggcgacgg aaaatgcctt
     3961 tgttctacaa taccatatga cgcccgatat tacgccatac atagaatatg actaccttga
     4021 ccgccagggt gtttacaacg gcagagataa tttatcggaa aacagttatc gcattggtgt
     4081 gtcatttaaa ctgtagtaga caggagacag tcacaatgaa taaaactata atggcgcttg
     4141 ctatcatgat ggcttcattt gccgcaaacg cgtctgtatt accggaaact cctgtgccat
     4201 ttaaaagtgg taccggagca attgataacg acactgtcta cattggttta ggtagcgcag
     4261 gtacagcatg gtacaagcta gatacacagg ccaaagataa aaaatggact gcgttagctg
     4321 cattccctgg tggaccgaga gagcaagcaa cctctgcatt tattgatggc aatctgtatg
     4381 tgtttggcgg cattggcaaa aacagcgagg gattgactca ggtatttaat gacgtacaca
     4441 aatacaaccc caaaaccaat agctgggtta aattgatgtc gcacgcgccg atgggcatgg
     4501 cgggtcatgt gacttttgta cacaacggca aggcttatgt tactggcggt gttaaccaga
     4561 atatcttcaa tggctatttt gaagatctca acgaggctgg aaaagattca accgctatag
     4621 ataaaatcaa cgcccactat tttgacaaaa aagcagaaga ttatttcttc aataagtttc
     4681 tgttgtcttt tgatccctca acacagcaat ggagttacgc tggcgaatcg ccctggtacg
     4741 gaacggctgg tgcggcggtt gtgaataaag gtgataaaac ctggcttatt aatggcgaag
     4801 ccaaaccagg attgcgaacg gatgccgtat ttgaacttga tttcaccggt aataatttaa
     4861 aatggaataa gcttgatccc gtctcatcac cagatggcgt cgctggcggt tttgcgggga
     4921 taagcaatga ttctcttata tttgccggag gggccggatt caaaggttca cgagaaaatt
     4981 accagaacgg taagaactat gcgcatgaag gcctgaaaaa atcatatagc actgatattc
     5041 atctttggca taacgggaaa tgggataaat cgggtgaatt atcgcaaggt cgggcctacg
     5101 gagtatcatt gccctggaat aatagtttat taattattgg cggtgaaact gcaggcggca
     5161 aagcggtgac ggattcagtt ttaatttctg tgaaggataa taaagtcaca gtacaaaact
     5221 aacgcttcag ggccccggta aaggggccct gctatcaatt tgtcatttgt attacgcgaa
     5281 aattatatga acgcaataat atcacccgat tattactatg ttcttaccgt tgctggtcag
     5341 tctaatgcca tggcgtatgg cgaaggactg ccattaccgg acagggaaga tgcgcctcat
     5401 tccagaatta aacaattagc gagatttgcg catacgcatc ccggaggccc gacatgtcac
     5461 tttaacgaca ttattccact gactcactgc ccacacgatg ttcaggatat gcagggttat
     5521 caccatcctc tggcaacgaa tcatcaaaca cagtacggca ccgttggcca ggcactgcat
     5581 attgcacgga aattactgcc cttcattcct gataatgcag ggattcttat cgttccgtgt
     5641 tgccgtggcg gatcggcttt taccgcgggc agcgaaggga catattcaga acggcacgga
     5701 gccagccatg atgcttgtcg ttggggaacg gatactccgc tgtaccagga tttagtcagc
     5761 agaacgcgag ccgcactggc aaaaaatccg cagaacaaat tcctcggcgt atgctggatg
     5821 caaggcgaat ttgacttaat gaccagtgac tacgcgtcac accctcaaca ctttaatcat
     5881 atggttgaag cctttcgtag ggatctaaaa caataccatt ctcagcttaa taatattact
     5941 gacgcgccgt ggttttgcgg cgataccacc tggtactgga aagaaaattt cccgcattcg
     6001 tatgaagcta tttatggcaa ttatcaaaat aatgttttag ccaatattat tttcgtcgac
     6061 ttccagcaac aaggtgaaag aggactgacg aacgcgcctg atgaagaccc ggacgattta
     6121 agcacgggat attacggttc agcgtaccgg tcaccggaga actggacgac ggcactgcga
     6181 agcagtcatt tcagcacggc agcccgtcgg gggattattt ctgacagatt tgtagaagca
     6241 attttgcagt tttggcgcga aaggtgagcg gggagtttat tcatggggga tgtccttgtt
     6301 cgttctcaga gtagacaaca aaaaacaaat gagatcaagg taatatctct tcacttgcgg
     6361 gctcgaacag caggcaattg ccaatataat aatctggcac aagggtgaaa aacgcgcacc
     6421 gcataaaccg ttgttattgc ttacagatta gccggatgcc ttaatagaca tccgcgcctt
     6481 ttcgattatg gctcggaaat ctacgaacat ctacacagtt tactggaacg ttttgggcca
     6541 cagcgttcgc agcaccgtcc tgatatgccg ttctggcgat tataagtcga cggattctgg
     6601 caaatagacg atgatgatat gcttaatgat gttcaaagaa attaggccat tattatatta
     6661 ttgcttgcat tttctttcat tcctaaaagc acccgccatt aatcccaggc gcattat
//
LOCUS       DAEEUE010000155         6615 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.155, whole genome shotgun sequence.
ACCESSION   DAEEUE010000155 DAEEUE010000000
VERSION     DAEEUE010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6615
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.155"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(97..2100)
                     /locus_tag="KLK84_003779"
     CDS             complement(97..2100)
                     /locus_tag="KLK84_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010927.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLSSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(2097..3233)
                     /locus_tag="KLK84_003780"
     CDS             complement(2097..3233)
                     /locus_tag="KLK84_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBE2010928.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALINVGAQIAAMTPGELASWLAENLQS"
     gene            complement(3226..5505)
                     /locus_tag="KLK84_003781"
     CDS             complement(3226..5505)
                     /locus_tag="KLK84_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010929.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(5516..6604)
                     /locus_tag="KLK84_003782"
     CDS             complement(5516..6604)
                     /locus_tag="KLK84_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBE2010930.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYLGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         1512 a         1823 c         1777 g         1503 t
ORIGIN      
        1 actggtgact ctcaccaggg gctatatagg atatgcctaa taccgtggcg tgagcagtcc
       61 ggaactggag tagaactctt agtaaaaagc actatttcat ccttgttgct gaagcatggg
      121 gaataattgt tcgcaaagca aaacaccgtt attcattgct tctacccgtg cctcgctttc
      181 tgtattacga aattgtgcca acacatgtgc cagccgataa aaacccaccg cggtgaggtc
      241 attcgccagc aactctgcct gattaatagc gctctgttcc tgatagcgcc agccgttatg
      301 gagcagttga ataagtaacg cctggcagcg catcagcaac tgatgagcag tagacggcac
      361 aggcagaacg ctggcagaag atagtgatac caccacaggc gcagtttctg cgtccagcgc
      421 ccaggcacga gtctttgtca tcattacctg tggttccagt gtcaattgcc catcaacaaa
      481 actgacaaag ccagaaacca gacacacggg gtcgtctgtt tgttgcaaaa gcgccgccat
      541 gcgttcaatg gcataaggcg cgctggctga ggctggcagt gataacgtca gcagattatc
      601 ttcaccttcg ccgctgatta cctgcgcatc cagcgtctgg cggctgctat cccaaccgag
      661 cgaaatacac tcagcgaccg gcagaataaa taagttatcg acctgattaa gaggccgtat
      721 gcaggcgggg ggacgctggc gtaaatattc ccgcaaagcc acaatgcccg gctggcgtaa
      781 cggcgcgctc aacatttgcc aggcatcagg cgacagcggt acaacgctgc ttaagcggtt
      841 gcgggtagct aacagcagct cgccatcggc actgcgtttt gctgcttgtg aaacaatttg
      901 cccacccgcc agtgcgccag cctgaaaact aaacagccga cgcgtagctg ccggtgagtc
      961 ttcctgttca cttcgcggcc aactgcgcga aaggtgcaaa atactgccgg tgtcgggatc
     1021 ggtaaaccag atgcgtaaac cataatgctc aatatcctgc cagcaacgca tacctaaaga
     1081 caccagccgc agatgatcaa gctttgcttc tccggcaatg ccagagccaa cgaccgtgcg
     1141 ccacggcaca ggaggaactt caccaacact gtcgctccgg gccatctctt gtgcgcaatt
     1201 taatcgactg tttaatgccg caagctgacg taagcattct ccggcatgat agtggctggc
     1261 gcgggcgtgg aaggcatcaa cgctggcgcg cagttgccgt agcgattcac tcacccatcg
     1321 ccagctgcag cgttccgctg cttgctgcgc gcggctgaaa gcggcctcgt agtgaataag
     1381 cggctggctg atgccgctca gccataatgc ctggcttagt tgttgaacat attgacgaca
     1441 cgttttgcct tcttcgctgg caaacggatc gtcagatgat gtgacgtgtt cgctgcgcat
     1501 ctgccagatt aaatgagtca attccgcttg ctgcgctttt gcctggacaa aggcctgtac
     1561 tgccagtacg acatgttcgc aaagtgtgcc ttcaatacaa tcacaacggg cgaaacgaat
     1621 actgctgcgg gaataaaaac gcacatcgct catcggtaag cgggcagagg gaatttcacc
     1681 cggcgcacag aacaactcaa tggtgatgct tttagcgacc agcgcctgtg cgcgtttgcg
     1741 ggtggcatcg ggaagggtag ccagttcttc cagccagatt gccggatccc attcctcttc
     1801 ttttcccgta ggctgggcgg tagcacaaag tcgttgataa cttaacacca gcatcacgcg
     1861 atgacggcac atgccgctgg ccccataggt gcactgagcc tctttcagtg cctggccgtt
     1921 cgccagctgg gtacggacac cgtcactgaa ggtggcgatt aaagcgctgt tctcatggct
     1981 gatttccggg acgttgccat tttccagttc cttaaggctg cgcttaacaa aaccggcatt
     2041 gcttaacgcc gtcagtgcct gcggtgtcag ttctaataat tccggacgta gtgaattcat
     2101 gactgaagat tctccgcaag ccatgatgcc agctcgcccg gtgtcatggc ggctatttgt
     2161 gcgccgacat taatcagcgc ctgggccgta tcgcggtcat agcaaggtgt tgctgtgcta
     2221 tcgagcgctg ccagtcccag cactttgatg ccgctctgga cacacttttt cacttggtgc
     2281 gtcagcaatg atgatgaacc cccttcataa aaatcgctca cgaggataat gacgcttttt
     2341 gcaggttgtt caataagttg ccgaccatac tccatggcac tggcgatatt ggttccgccg
     2401 cctaattgca ctttcattaa taactctacc ggatcggcaa cgtctgccgt gagatcaacg
     2461 acgcttgtgt caaacgccac cagatgggta cgaatgccgg gtaactgcca taaacaggcc
     2521 gccatcaccg cagagtggat caccgaatcg accatcgatc cgctttgatc aaccagtaag
     2581 accagttgcc attgctcgct ttggcgttta atgcggctgt taaagcgggg gaattcgata
     2641 tacaacttgc cgtgttgcgg gtgccagtgt tgcaggttgg cgcgcagagt acttttgaaa
     2701 tcaaagtttc gcgccagtgg aataaatgag cggcgacgac gatcgcggac accagaaaaa
     2761 gcctgacgaa cttcctttgc cagtcgcgcc ataatttctt ccacaacctg gcgcacaatc
     2821 ctgcgggcgg cagccagtac ttcggggttc atcagatgtt tggtgtgcaa aacggcgcgt
     2881 agcaggcttt cggaaggctg catacgttcc agcacgtcga gatttgtcac cacatcttca
     2941 atgccgtagc gcagtacggc atcgctttcc agccgctcaa tcacctgttg cggaaacagc
     3001 gtgtgaatac tgttgatcca ctcaggagtg gtgagatttg agccacctaa tccactagag
     3061 cgttcaccac gctggagccg ttcaggatcg cgcccataca gccactccag cgcgtgatct
     3121 atctgccggg cgttgtcatc cagcccacaa agcgtcgttt ctgccgcttc gccaagaatt
     3181 aatcgccagc gttgtagctc acgggtggtc agaagatcgt tcagttcaga catggaaaac
     3241 tccccagtgt tgcagcgatg ccagtgcctg ctgttcgagt tgttgatgat gtgcaatggc
     3301 ttgcggtgga caatgcaacg gcatttgcag tgccgaaacg ggaagttgcg ccagttgata
     3361 atgctcaagc acctgatgcg ccagcgtccc gcgttctcgt ggcggtagcc aggccattgc
     3421 cgcgcgtaaa tcgggcaggg cattggtaaa atcggcatca cttagttgat ttaaatgact
     3481 gctgaaaccg gcgataaatg ccggctgaca ggccagttga tgacgggcta atgccagcaa
     3541 accgtgcagc gcctcgccgg attgtgccgg ggataactgc gccagcatcg tcagcgccgc
     3601 ttcggcacta gcgttgggat gttccagacg catcagcgca ccaagagtcg cgccgcgatc
     3661 cagtgccagg acatgaatcg cctgactgtg tcgctccagc aaggctaccg ccgcagaaag
     3721 cgaaacgccg gggagttgca caccgctatg tagatcgcgc aggatatgac acaacgcttg
     3781 ccagctatgc aagtgagcgt gaaactcctt ttcatccggt ctgccgttgg attcacacag
     3841 ccacagcgtg cgatcgatag ccgcgcataa cgtcgtttgt aatatctgcg cgccttgcat
     3901 accgctaatt tcatccagcc gccataaggc atatagcact tccagcgcct ggcccatttc
     3961 ggcaaattga ttttcctggg cgattaataa tgtgagttgc tccagtaatt gttgactgaa
     4021 ggacgccaga cccgctgacg ccgcttggct aagacatgtg gtaatgctgc cgattccacc
     4081 cgcgtccagc atatcggctt ctaatttatg gcgtgcggct tcctggagtg tggcaccaaa
     4141 gcaggcggcc tcaatcaatg ccgcatgctg gctaagcggg cgggttaatt tccagcgttc
     4201 ttcaccgttg cctgcaagtg tcagtgtgct tccctgctgg cgtacaatcc ctgggatctc
     4261 cagtattgcc agccgatgta acacctgact ttgcgctagt ccattggggt taaagcgatt
     4321 taaggtaagc tccgccggaa gagagattgc ggtacgttcc agttcgcaag tgacatcttt
     4381 tggcagaggc ggttgtggcg tagaaggggc aagtttaccg aatccgtcac ccgctaatgt
     4441 gtctatcaac gttagcagaa tcggatcggt atcgggatga ataacgccgc gatagctcca
     4501 cggcaacggc gcattcaggg cttctttaat cagcgatcct gctatggcat ccagccagtc
     4561 agtgcgtaac ggtagtgtat gaccgcgcaa ctgtgccagt gccatcgcat gcagatgagc
     4621 ggcagccata tccgcggtcg aagcaggcaa cttgtgctgg cgcaaacggg tgagaaccgt
     4681 ttttagcagt tgttctcccg cctgctgtaa gccccactgc cagcaccagt tttgccagac
     4741 cggggcaggc attcctgaaa ggtatcctgc cagcacatca aggcgctttt cactgtaggg
     4801 ggtgagataa caacctgtaa cggcatctgc cagcgacggc aattctggcg tgttaattac
     4861 ctgcggacat tcgcgccaca tttttgccag tgccggagcg tgccagccac cgcagacaac
     4921 taacacgtcg ccattatttt gctgcatcgc ccatccaatc cagcgggcca taaaggcttc
     4981 gcgctgacga tttagagcat cgccggggga gtcgccccgc agttgggcaa aatagtgcgc
     5041 cagtgcagag ggtaatgcag tttgctggct ttcatcttcg aacaagtgat cccacagggt
     5101 gtcgctgtta tccatgcggg tggcacgcag cagcaaggtc tggctttcct cttgcgtatc
     5161 aggcgaatcg tcctcttctt cactctgcgc ccagcaaggc aaatcgatga agtaagtttg
     5221 tgcctgaata cgacgatcgg cttgtagcgc ctgccactcc ggcgaaaatt cagcaaatgg
     5281 cgtccaggca ccacgcccgg gggctgcacc gtcctgatac tggcaataac tgtaaatagc
     5341 taccggaagc tggtgggcta aaaacagttc gtctacccgg tcattaaaat cagcagggcc
     5401 ttcaatcaac acgtatcgtg gccgctggct ttcgattaac gatttcacca gccgggcgca
     5461 ggccggacta tgatgccgga tgccgacaat taacggctcg ctcatgcatc tttcctcacg
     5521 gcaggcggtg gcgggcttga taataagcct gccagtgagc ttctttatgt gtggcgacac
     5581 gctgttcgaa gtaacggcgc agacgagcgc gatcttcttc gttatctttg acgatggttc
     5641 cggcaatgca gtccaccagg tcggctggct cacctgcgcg attcgctaaa aaccaggcgc
     5701 ggacgcctac ggcgtgggcc acgttcacgg cttcagcggt ggacataatc gctgttagtg
     5761 tatccattga cgttttcttt tcgccgttgg cacgcagatc gcggaaggtg cggaccagta
     5821 actccagtac cgcgtcaggc actttatgcg gaataccgct atgggccagc aaacgcgccg
     5881 aagcactggc gacaagctcc agctcctggg cgaaatccat aatcggaaag acagtttcaa
     5941 aatcgaagcg acgctttagc gcagcactca tttcattgac gccgcgatcg cgggtattgg
     6001 cagtggcaat aatattaaag ccttctcgcg catagagctg actggcttca ccagtgagtt
     6061 ccggcaccgt catcaccctg tcagaaagca tacctaacag acagtcttgc acttccagcg
     6121 gtgtacgcgt tatctcttca aaacggacga ttttgccgtc gcgcattccc aggtaaagcg
     6181 gggcagggac cagcgcttcc gttgatggac catgattgat aagcagggcg tagttccagc
     6241 cgtacttgat ttgatcttcg gtagtagatg cgccgccctg gatggttaac ccggcatcgc
     6301 cgctaataga ggttgccagt aattcagaca ggagagattt tgcggtgcca ggttccccca
     6361 ccaacatcaa accgcgtccg gtggcaagag tgactaacat acgttcgaca gcggagggac
     6421 taatcaccac tttacggcta atattttgcg cgctatcgcc aaggataaaa gcacgggccg
     6481 caggcaaact taactgccaa ccgggcgggc aaggtgcgtt atcattttgt tttaattttg
     6541 ccagttcatc ggcgtataac acagcagcag gcggacgctg cagatgatta ttctgcggtg
     6601 acatatattc atccc
//
LOCUS       DAEEUE010000156         6403 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.156, whole genome shotgun sequence.
ACCESSION   DAEEUE010000156 DAEEUE010000000
VERSION     DAEEUE010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6403
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.156"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="KLK84_003783"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="KLK84_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HBE2010931.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="KLK84_003784"
     CDS             complement(1433..1759)
                     /locus_tag="KLK84_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HBE2010932.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="KLK84_003785"
     CDS             1894..2235
                     /locus_tag="KLK84_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HBE2010933.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="KLK84_003786"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="KLK84_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="KLK84_003787"
     CDS             complement(2833..3543)
                     /locus_tag="KLK84_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HBE2010934.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="KLK84_003788"
     CDS             3651..3956
                     /locus_tag="KLK84_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HBE2010935.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..4478
                     /locus_tag="KLK84_003789"
                     /pseudo
     CDS             4155..4478
                     /locus_tag="KLK84_003789"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5034..5612
                     /locus_tag="KLK84_003790"
                     /pseudo
     CDS             5034..5612
                     /locus_tag="KLK84_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
BASE COUNT         1706 a         1628 c         1501 g         1568 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagcgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actgggttgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatatataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgataggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag gtacgcgcct
     4441 gtttgcgcgg tcgctccatc cgccggcgta ttaatcatcc cgatcgcttg aatgacccaa
     4501 tgaaacgcgt gggtaaacgc ggtgaaggca aattcgaacg gattagctgg gatgaagccc
     4561 tggatactat cgccagtagc ctgaaaaaaa ccgtcgaaca atatggcaat gaagctgtat
     4621 atattcagta ctcttcgggg attgttggcg gcaatatgac ccgctcttcg ccatcagcct
     4681 cggcggtcaa acgcctgatg aactgctacg gcggttcact caatcagtat ggctcctaca
     4741 gcaccgcgca aatttcctgt gccatgccct acacctacgg cagtaatgat ggcaacagca
     4801 ccacggatat tgaaaacagc aagctggtcg tgatgtttgg taacaacccg gcagaaaccc
     4861 gcatgagcgg tggtggcatc acttatcttc ttgaaaaagc gcgcgagaaa tcgaacgcca
     4921 aaatgattgt tatcgatccg cgatataccg atacggctgc cggtcgtgaa gaggaatggc
     4981 tccctattcg cccgggcacc gatgccgcgc tggttgcggg tattgcctgg gtattgatta
     5041 acgaaaatct cgttgatcaa ccttttctcg ataaatactg cgtcggctat gacgaaaaaa
     5101 ccttacccgc agatgcaccc aaaaatggtc actataaagc ctatattctt ggtgaaggtg
     5161 acgataaaac agcaaaaacg ccgcagtggg cttcgcaaat taccggtatc ccggtagacc
     5221 gtatcatcaa actggcgcgt gaaattggca caacaaaacc cgcttatatc tgctagggct
     5281 gggggccgca acgccaggca aacggcgagc tgactgcacg cgctattgct atgttaccta
     5341 ttttgacggg caatgtcggc atcagcggcg gaaatagtgg cgcgcgtgaa tcgacctata
     5401 ccattacgat agaacgccta ccggtgctgg ataatccggt caaaacgtca atctcctgct
     5461 tcagctggac ggatgctatc gatcatggcc cgcaaatgac ggcactccgc gacggtgtcc
     5521 ggggcaaaga taaactggat gtgcccatta agttcatctg gaacttcaag gacttgaagc
     5581 ggacccttag taggcctgag gtcctgcatt gagtcaagac cttgtgtcat tctttgtgcc
     5641 agacttgtgt cattagacgg gatgtctaga cggctggtca gcccccccgg tggcctcggt
     5701 ctggacttga ggtgggccta tggggggaag tctgctggct taatgtatta gatggtcatt
     5761 cacatttttc tgtcagaaac catcggtccc actgtgagtc caaacctaag gacctagaag
     5821 tccacccaca gccttagaca gagtgtcgaa cacatccaca ccgagtcgac cagagaggac
     5881 catgtagaga cccacagaga ggaacacgag tttatagacc agagtcagga gggacttcag
     5941 gtctgcggaa gtcatagaca taaagcccct atctgttatg ttatattatt atattaacaa
     6001 caggtccaga ccattggtcc agaccatagt tagttaatca cagtgatagc ccaccacctg
     6061 aagtcctgct gatattcagt ccagacttac ctctgctcat ggtcttacct ccctcggtct
     6121 cctgaccttg ggtatcgact acagcttgct gagcgtcatc tggaggagct acaggaggcg
     6181 ggaccgaagc cccaccttga gtcccaccac cacctgtcag gcccttggcg atggacctga
     6241 agcctttact aatgctctta cccattggtc ttacgcttcg gtcttggtga acttcaggcg
     6301 tttgactaca ggcaggacgg cagtagctga gccagtgccc atcttgacgg tcgtagcagc
     6361 ttcagcgaca acgaacactt taccgaggat ttccaagtcg tgg
//
LOCUS       DAEEUE010000157         6355 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.157, whole genome shotgun sequence.
ACCESSION   DAEEUE010000157 DAEEUE010000000
VERSION     DAEEUE010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6355
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.157"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..370
                     /locus_tag="KLK84_003791"
     CDS             <1..370
                     /locus_tag="KLK84_003791"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010936.1"
                     /translation="TTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPS
                     GSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNA
                     LINLLAQQSRFADLKLSNYK"
     gene            complement(458..1102)
                     /gene="cheZ"
                     /locus_tag="KLK84_003792"
     CDS             complement(458..1102)
                     /gene="cheZ"
                     /locus_tag="KLK84_003792"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HBE2010937.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1113..1502)
                     /gene="cheY"
                     /locus_tag="KLK84_003793"
     CDS             complement(1113..1502)
                     /gene="cheY"
                     /locus_tag="KLK84_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HBE2010938.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1517..2566)
                     /gene="cheB"
                     /locus_tag="KLK84_003794"
     CDS             complement(1517..2566)
                     /gene="cheB"
                     /locus_tag="KLK84_003794"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HBE2010939.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2569..3429)
                     /gene="cheR"
                     /locus_tag="KLK84_003795"
     CDS             complement(2569..3429)
                     /gene="cheR"
                     /locus_tag="KLK84_003795"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBE2010940.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3448..5049)
                     /gene="tap"
                     /locus_tag="KLK84_003796"
     CDS             complement(3448..5049)
                     /gene="tap"
                     /locus_tag="KLK84_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HBE2010941.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5095..>6355)
                     /locus_tag="KLK84_003797"
     CDS             complement(5095..>6355)
                     /locus_tag="KLK84_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HBE2010942.1"
                     /translation="TELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRD
                     IVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHICEIAGGNLA
                     NTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTE
                     QQASALEETAASMEQLTATVKQNADNARQASQLAQSASDTAQHGGKVVDGVVKTMHEI
                     ADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRNLASRSAQ
                     AAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRVTDIMGEIASASDEQSRGI
                     DQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQAVSAFRLAASPLTNKPQT
                     PSRPASEQPPAQPRLRITEQDPNWETF"
BASE COUNT         1439 a         1837 c         1525 g         1554 t
ORIGIN      
        1 cactacgcta atgagttggg atgccgtgct ctatgatcta cgtagtgaaa aaattatcag
       61 taaagataat tacttgaccg acttaaaccg cagacatctt gaacttgttc ttcatactaa
      121 ccccaatcca agcggttcgg taacgaagat tttacgcact atttttacta agcaggtaaa
      181 gagtttaggg ccggaactct gtcatttctt gcaccatgaa ctgacgaaaa aacgttatga
      241 agtatggcat cactacgatg tactgcacta ccagtcatcg tcattttctc gttcacaatt
      301 taatgcattg ataaatttac tggcgcaaca atcacgcttc gctgatctaa aattatcaaa
      361 ctacaaataa ccatcctcca cgtactccct gaacgatacc cgccaccctg ctatcggctt
      421 ggcatcatcg cgcaggccga agttcaggca atacaaatca aaatccaaga ctatccaaca
      481 aatcgtccac ctgatcctga ctggctacca cgccggcttt gctggtatcg acctgaggtc
      541 cattaagcaa actctggttt tcacgttttg gacgcgactc ctgttccggg atgttttcca
      601 acagcaccat cagcaactgg cgttcgatct cctgaatgac atccatcatc cgcttaataa
      661 cctgcccggt gagatcctga aaatcctgcg ccatcatgat ttccagcagt tgcgcgttag
      721 taaagctggt atgcgcgggt acatctgcca gaaattgtcg tgtatctgtt accagttcac
      781 gggcgtcggc aaggtcaatc ggatcggcaa accagtcatc ccaacgttgg gttaacgctt
      841 ttgctgattt ctccatttga tcctgatgcg gttgtgacgc ctcaacactg ttcagcgccc
      901 gctccgcagc ctgggcggtc atctgcacaa catagtacaa acgatcgcgc gcatcgggga
      961 tggcttccgc cgcttcggca atggcctgat ccagccccag ttcccgcaaa ctgtcgcgca
     1021 gcatatgcgt caggctgccg atgcgcgcaa tgatatcgcc agctgaatgc tcgtcagcag
     1081 gtttgattga tggttgcatc atagtcgcat cctcacatgc ccagtttctc aaagattttg
     1141 ttgagttttt cctccagcgt cgcggcggta aatggcttca ccacatagcc actggccccc
     1201 gcttgcgccg cagcaatgat gttctctttc ttcgcttctg cagtcaccat taacactggc
     1261 aatgccgaca tcgcgccatc cgcacgaatt gttttcagca attccaggcc atccatattg
     1321 ggcatgttcc agtcggagat aacaaatcca taaccgcctg cctgcaactt attgagagcg
     1381 tcgacaccat cttccgcttc ctcaacatta ttgaatccca gctctttcag caggttacgc
     1441 actatgcgtc gcatggtgga aaagtcatcc acaaccaaaa atttaagttc tttatccgcc
     1501 atttcacact cctgatttaa atacgtatcg cctgtccggc actaattgtt gccaacattt
     1561 gctggcttac ctggctaaga tcgaccactt cgcagacacc acccatattg atggcctcgc
     1621 gcggcatgcc gaacaccacg caacttgctt cgttttgcgc aagggtccat gcccccgcct
     1681 gacgcatcgc caacattccc gccgcgccgt cgttgcccat accggtcagg atcaccccaa
     1741 ccgcattacg ccccgcctgt ttggcgacag aatggaacaa cacatctacc gaaggccgat
     1801 gacggttaac cgccgggccg tcgtgaattt tgatttgata atttgcgcca ctacgcgcca
     1861 gttccatatg ccgatcgccc ggcgcaatat aggcatgccc cggcaggaca cgttctccgt
     1921 cttcggcttc tttaaccccg atctggcaaa gcttattaag tctgtcggca aaagagcggg
     1981 tgaaaccggg cggcatatgt tgggtaatta acaatgccgg gctggaaagc ggcaacggtt
     2041 gcagtacgtg acgaattgcc tcagttccac ccgttgaagc accaatcgca atcagttttt
     2101 cagaactcaa caacggccct gccttcagcg ttgtcggtgc tgacaatggc ttatgtgctg
     2161 caaggctcgc ctttgctgcc gtacgcacct tttcagcaat catttcgcta tacgccagca
     2221 ttccttcgcg aatacccagt tgcggtttgg tgacaaaatc tattgccccc agctccagcg
     2281 cgcgcagcgt gacttctgat cctttgccgg tcaaggaaga aaccatcaca acgggcattg
     2341 gacgcaaacg cattaatttt tcgaggaaat ccagtccgtc catccgcggc atttcaacat
     2401 ccagcgtcag cacatcggga ttgaatttct taatcaagtc acgcgcaacc agcggatcag
     2461 gcgcggtcgc caccatttcc atgtcgctat ggctgttgat gatttctgtc atgatctggc
     2521 gcatcagtgc cgaatcatcg acagataaca ccctgatttt gctcatcgtt aatccttact
     2581 tagcgcatac accgtctgac cacgcagcgt gaagcggcgc tcgaggtggc taaagttttc
     2641 agagtgaccc gcaaacagca atccgtcggg tttaaggagc ggaacaaagc ggcgcaaaat
     2701 ctcctgctgg gtagtttgat cgaagtagat catgacgtta cgacagaaga tcgcatcaaa
     2761 cggccccggc acggtgtact gtttcgccag taaattcagc ggggcaaaat caacatagtt
     2821 cgccagctcc tgacgcacgc gtaccagccc ttcatgcggt cccgtccctc gcatgaaata
     2881 ccgttgcagt tgctgcggcg tcaggttttt caactcttca tggcgataga taccgcttct
     2941 ggctttttcc agcacttcgg tgtcgatatc actggcaaac actttccagc gtccgggcac
     3001 ggtgcccaat gtgtcagcca gagtcatcgc aatgctgtac ggctcttcgc cggtcgaagc
     3061 cgccgcgctc catacgcgat cctcgccaga acggcgacgt gcgtgatccg cgagcagagg
     3121 gaaatgatga gcctcacgga aaaatgccgt cagattcgtg gtcagcgaat tgataaacgc
     3181 ctgccactca ccgctgtgct gattagattc cagcaagttc agataatgac cgaaatccgt
     3241 cagtcccagc gaacgcaaac gacgaaccag tcggttgtaa accatgtcgc gtttatggtc
     3301 agccagaacg atcccggctc gttgatagat caattgactt atccgccgaa aatgcgcgtc
     3361 ggaaagcgcc aggcgctcgg tcatctgtaa caataaagac gtttgcccac agggcagaga
     3421 tgaagtcata gcgccttctc aatcacttca ggataccact ggcgcaattt gtaactgcgc
     3481 cgactcatgt cgtgctactt catgttcttc aagggtaaat accgccacac gcgacgaaag
     3541 atggtcggcc tgattcgcca gttgttccgt tgccaccgcc gcttcttcta ccagcgagga
     3601 gttctgctga gtcacctgat ccatctggct gacagcctgt gcaacctgct caatcccccg
     3661 ccgttgttct tccgacgccg aagcaatttc tcccataatg tcgttcacgc gggtcaccga
     3721 actgacaata tcggtcatgg tcgcggcggc gttattcacc agtttcgaac cctgctggac
     3781 acgattgact gactcttcga tcagcccttt gatctctttt gctgcctgcg cgctacggct
     3841 ggcaagattg cgcacttcac ctgccactac cgcaaaacca cgcccctgct ctccggcgcg
     3901 agccgcttcc actgccgcat tcagggccag aatattggtc tggaaagcaa ttccgtcgat
     3961 aacgctgata atgtcgccaa ttttttgcga gctggtggcg atctcctgca tggtgtgagt
     4021 catggtactg acctgaacac cgcccgcctg cgccgttgtc gcggcatttt ttgccagttc
     4081 cgacgcctgt cgtgcgttat cggcgttttg ccctaccgtg gcggttaatt gctccatact
     4141 ggcggccgtt tgtgccagcg atgccgcctg ctgttcggta cgacttgaga gatcgttatt
     4201 gcctgcgaca atctccgcga tcccaatgtg catctcatgg cttcccttac gcacatcact
     4261 caccgtccca cgcaaagcct gctgcatggt cttcaggctg gcaaaaatgg cggtgatctc
     4321 attacgacca tataccgcaa tcggacgcgc cagattaccc gcagcaatgc tgtcaaaatg
     4381 gctaccgata atggccagtg gttgaacaat catcttgcgc gtccaccaca gcgcactgct
     4441 gatgtagatc gccgcaacaa taatcatgct gataaacacc agcgccgaaa tctgatagtt
     4501 acgctggctt tgcgcactgg cggcttccag cacatggttg atctccagct gccaggcctc
     4561 aaagttaacg tcaaacgcat tctgtgatcc ctgcaccggc gcagtgagga aatccgaaag
     4621 ttgattactt tcaagccagg tcgcctgatg ttcgagatcg ttgtgccagc gggcaaaact
     4681 tttctccgtc tctttttgca atgccctgac gtgctcgttg cccgcagtca tcgccataaa
     4741 acttttaaac agcgtggtgg attgcgtcag actggcgcgc gccgtcgtca tcaacgtttt
     4801 aatgtcatcc gccggatagc taagcgctgt cagagtgcct gctttgttca gcgcggtact
     4861 ggcctgtaac attaccgccc gagtttgcgc taatgccgca cgttgctgat tgctctgctc
     4921 gccgtgattc agtcgttgca aatcgtcgcg aaatgcccaa aaagacatgc cgttactgcc
     4981 aatctgcaag atcccgcaga gaatcaaaat taaaaacagc gtggtcgaaa ttcgaatacg
     5041 attaaacatc aacgctcccc atcaggcggc aatgaccgcg ttagtaaaaa ctcgtcaaaa
     5101 tgtttcccag tttggatctt gttcagtaat tcgcagtcgt ggctgtgccg gtggttgctc
     5161 actggcagga cgggatggtg tttgcggttt attggtgagt gggctggctg ccagacggaa
     5221 cgcggaaacc gcttgcgtta aacgactcgc ctgttcttcc agcgcagctg cggcggcagc
     5281 tgattcctgc accagcgatg cgttctgttg cgtgacgcga tccatttccg aaaccgccaa
     5341 tgcgacttga tcgatgccac ggctctgttc atccgatgcc gatgcaatct cgcccataat
     5401 gtcagtcacg cgagtgacag cattgacgat attgttcatt gtttccccgg cgctttcgac
     5461 cagcaccgaa ccggtatcaa tgcgcgagac ggagtcttca atgagggctt tgatctcttt
     5521 tgccgcctgg gcgctgcgac tggcaagatt acgcacttca cccgccacca cggcaaaacc
     5581 acggccctgt tcacccgcac gcgcggcttc aaccgcggca ttcagcgcga ggatattagt
     5641 ctggaaggca ataccgtcga taacgctgat aatgtcggca attttcttcg aactatcggc
     5701 gatctcatgc atcgttttca ctacgccatc caccactttg ccgccgtgct gggcggtgtc
     5761 ggaggcactt tgcgccagtt gcgaggcctg gcgggcgtta tcggcgtttt gcttcactgt
     5821 cgcggtgagc tgctccatgc tggcggcagt ttcttccagc gcggatgcct gctgttcagt
     5881 acgggaggaa agatcggtgt tgcccgccgc aatttcacgg gtaccggcat agatggcatc
     5941 tgaaccttcg cggacatgag tgacggtgtc agtcaaagag cgttgcatat gtgaaacgct
     6001 ctgcgccagg tcacccattt cactgcgccc gtcaatggtc agggtattcg ccaggttacc
     6061 accggcgatt tcgcaaatgt gagcaataat ttttgccagc ggagtaagca acatacggcg
     6121 aatgccgtac cacgccacca gcagaatcaa taccaccacc agcgcgataa ccgccagttg
     6181 ccactgggca aatcggtaat catctgcgtt gtcagtgacg atatcgcgat acagtttttc
     6241 actgctgagg gcgtactgag caaacgcttc gcccattgca ttttgcattc cctgggttgg
     6301 ctgagcgaaa taagctccag tattgccata atcaagataa tcaatcagtt cagtt
//
LOCUS       DAEEUE010000158         6336 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.158, whole genome shotgun sequence.
ACCESSION   DAEEUE010000158 DAEEUE010000000
VERSION     DAEEUE010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6336
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.158"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            254..1642
                     /gene="narU"
                     /locus_tag="KLK84_003798"
     CDS             254..1642
                     /gene="narU"
                     /locus_tag="KLK84_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HBE2010943.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVTVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1724..5464
                     /gene="narZ"
                     /locus_tag="KLK84_003799"
                     /pseudo
     CDS             1724..5464
                     /gene="narZ"
                     /locus_tag="KLK84_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            5461..>6336
                     /gene="narH"
                     /locus_tag="KLK84_003800"
     CDS             5461..>6336
                     /gene="narH"
                     /locus_tag="KLK84_003800"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HBE2010944.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDL"
BASE COUNT         1493 a         1685 c         1663 g         1495 t
ORIGIN      
        1 attcacgatc tgaagggttg aaacatgaca tacgttcaaa gattaacccc cgtttgaagg
       61 ttagcttgtt agttacattt agtaacacat atctggctac ttaatcccca ttctgccccc
      121 gcggttacgc tttcttatct attctttcaa tcagcacttc acccaattca tccttaattc
      181 taataactgg ctattgcgcc ttacctcctg ttttgtcttt tttcattacc aatgtgtgca
      241 tatgaggaac aatatggcac tgcaaaatga gaaaaatagt cgttatcttt tacgcgactg
      301 gaaaccagaa aatccggcct tctgggaaaa taaaggaaaa catattgctc gaagaaacct
      361 ctggatatca gtcagttgtc tacttcttgc cttctgtgtc tggatgctat ttagcgcagt
      421 taccgttaat ctcaataaaa tcggttttaa ttttactacc gatcaactct ttttattaac
      481 cgcattaccc tccgtttctg gcgcattatt gcgtgttccc tactccttta tggtgcctat
      541 attcggtgga cgccgatggc cggtttttag tactgcaatc ctgattattc cttgcgtctg
      601 gctcggaatt gccgtgcaaa atccgaatac tccttttggg atatttatcg ttatcgcttt
      661 gctatgcggt tttgcaggtg caaactttgc ttcgagcatg ggcaatatca gtttcttctt
      721 tccaaaagcc aaacaaggga gcgctcttgg gattaatggc ggattaggaa acttaggtgt
      781 aagtgtaatg cagctggttg caccgctggt catttttgta actgtatttg cctttctcgg
      841 cgtcaatggc gtaccgcagg ccgacggttc ggtgatgtcg ctggcgaatg ccgcatggat
      901 ttgggtaccg ctactggcga ttgccacgat cgccgcctgg tcagggatga atgatatcgc
      961 cagttcacgc gcctcaattg ccgaccagct ccctgtctta caacgcctgc atctctggct
     1021 gctgagcctg ctttaccttg ccaccttcgg ttcgtttatc ggtttttctg cgggttttgc
     1081 catgctggca aaaacccagt tcccggatgt gaatattctg cgcctggcgt tctttggccc
     1141 atttattggt gccatcgcgc ggtcggttgg tggtgctatt tccgataaat tcggcggcgt
     1201 gcgggtgacg ttgatcaact ttatttttat ggcgattttc agtgccctgc tgttccttac
     1261 cttaccgggc acaggctccg gtaatttcat cgccttttac gccgtattta tggggctgtt
     1321 tctgaccgcg ggtctgggaa gtggttctac tttccagatg atcgccgtca tctttcgcca
     1381 gataaccatt tatcgggtga agatgaaagg cggtagtgat gagcaagctc agagagaagc
     1441 cgtcaccgaa acggcggcaa cgctgggctt tatctcagcc attggcgcag tgggcggctt
     1501 ttttattccg caggcatttg gcatgtcgct caatatgacc ggctctccgg tcggcgcgat
     1561 gaaagtgttt ttaatcttct acatcgtttg tgtgctgctg acctggctgg tttatggtcg
     1621 gcggaagttc agccaaaaat aagagatcga tgcctgatgt acaaccatgt cgcatccgac
     1681 aaggcatcag atcccaattc attcctggag caggagtcat gtcatgagta aacttttgga
     1741 tcgctttcgc tacttcaaac aaaagggcga aacctttgcc gatggtcacg gacaggtgat
     1801 gcatagcaac cgcgactggg aggacagcta tcgccagcgt tggcagttcg acaaaatcgt
     1861 gcgttccacc cacggcgtta actgtacagg ctcctgtagc tggaaaatct acgttaaaaa
     1921 tggtctggtg acctgggaaa tccaacagac cgactacccg cgcactcgcc ctgacctgcc
     1981 caatcatgaa cctcgcggct gcccgcgtgg cgcaagttac tcctggtatc tttacagcgc
     2041 taaccgcctg aaatacccgc tcattcgtaa acgactgatt gaactgtggc gcgaagccct
     2101 caaacaacac agcgatccgg tactggcgtg ggcatcgatt atgaacgatc cgcaaaagtg
     2161 cctgagctac aaacaagtgc gtgggcgcgg cgggtttatc cgctccaact ggcaggaact
     2221 aaaccagctg attgccgccg ctaacgtctg gaccatcaaa acctacggcc cggatcgcgt
     2281 tgccggtttc tcgccgatcc cggcgatgtc gatggtttct tacgccgccg gaacgcgtta
     2341 tctgtcgctg cttggcggca cctgttaaag tttctacgac tggtattgcg acctgccgcc
     2401 cgcctcgccg atgacctggg gcgagcaaac cgacgtaccg gaatctgccg actggtataa
     2461 ctccagctac atcatcgctt gggggtctaa cgtaccgcag acacgtacgc cggacgccca
     2521 cttctttacc gaagtacgct acaaaggcac taaaaccatc gccattaccc ctgactactc
     2581 tgaagtggcc aaattgtgcg accagtggct ggcaccgaaa caaggcactg atagcgccct
     2641 ggcgatggca atggggcatg tgattttaaa agagtttcat ctcgataatc ccagcgacta
     2701 ctttatcaac tactgccgcc gctacagcga catgccgatg ctggtaatgc tggagcctcg
     2761 cgacgatggt agctacgttc ccgggcgcat gatccgcgca tctgacctgg tggatggact
     2821 gggcgaaagc aacaatccgc agtggaaaac cgtagcagtt aataccgcag gtgaattggt
     2881 agtgccgaac ggttcgattg gtttccgctg gggagaaaaa ggcaaatgga atctggaatc
     2941 cattgccgcc ggtacggaaa ccgaattgtc gttaaccctg ctcggtcaac atgacgctgt
     3001 tgcaggcgtg gccttcccct actttggcgg cattgaaaat ccgcattttc gcagcgtaaa
     3061 acacaatccg gtgctggtgc gccaattgcc cgttaaaaac ctgacgttag tcgatggcaa
     3121 cacctgtccg gtggtcagcg tttatgattt ggtactggcg aattacggcc tcgatcgcgg
     3181 gctggaagat gaaaacagcg cgaaagatta cgctgaaatc aaaccgtaca ccccagcctg
     3241 gggtgagcaa attaccggcg taccgcgcca gtatattgaa accatcgccc gcgaatttgc
     3301 cgataccgcc cataaaacgc atgggcgctc aatgattatc ctcggcgcag gtgttaacca
     3361 ctggtatcac atggacatga actaccgcgg gatgatcaat atgctgatct tctgcggttg
     3421 tgtcgggcaa agcggtggcg gctgggcaca ctatgtcggc caggaaaaac tgcgcccaca
     3481 aaccggctgg ttgccactgg cctttgcgct cgactggaac cgaccaccgc gccagatgaa
     3541 cagcacctcg tttttctaca atcattccag ccaatggcgc tatgaaaaag tctctgctca
     3601 ggagttactt tcaccgctcg ccgatgccag taagtacagc ggtcatctga ttgatttcaa
     3661 cgttcgcgcc gaacgtatgg gctggctacc ttctgcgccg cagctggggc gtaacccgct
     3721 cgggattaaa gctgaagccg acaaggccgg attatccccc acagaattta ccgcccaggc
     3781 gctgaaatcg ggcgatttac gtatggcctg cgaacaacca gatagcggca gcaatcatcc
     3841 gcgtaatttg tttgtctggc gctctaacct gcttggctcc tccggcaaag gccacgagta
     3901 tatgcagaag tatctgctgg ggaccgaaag cgggattcag ggcgaggaac tcggtgccag
     3961 cgacgggatc aaaccggaag aagtcgagtg gcaaactgcg gcgattgaag gcaagctcga
     4021 cctgctggtg acgctcgact tccgcatgtc cagtacctgc ctgttctccg atatcgttct
     4081 gcccaccgcc acctggtatg aaaaagacga tatgaacacc tcggatatgc atccatttat
     4141 tcatccgctt tctgcggcgg tcgatccggc ctgggaatca cgcagcgact gggaaatcta
     4201 caaaggtatt gccaaagcat tttcgcaagt gtgcgtgggc catcttggca aagaaaccga
     4261 cgtggtatta caaccactgc tgcatgactc tccggcagag ctctcacagc cgtgtgaagt
     4321 gctcgactgg cgcaaaggcg aatgcgatct gatcccgggt aaaaccgcgc cgaatattgt
     4381 ggcggtggag cgcgactacc ctgctacgta tgaacgcttt acctcgctcg ggccattgat
     4441 ggacaaactt ggcaatggcg gtaaagggat ttcgtggaat acccaggatg aaatcgattt
     4501 cctcggcaaa ctcaattaca ccaagcgtga tggcccggca caggggcgtc cgctgattga
     4561 caccgccatt gacgcttcag aagtgattct ggcactggca ccggaaacca acggtcatgt
     4621 tgcagtcaaa gcgtggcagg cgctgggcga gatcaccggg cgcgaacata cccatctggc
     4681 gctgcacaaa gaggacgaga agattcgctt tcgcgatatt caggcgcagc cgcgtaaaat
     4741 tatctccagc cccacatggt ccggtctgga aagcgatcac gtatcctata acgcgggata
     4801 caccaacgtt catgagttaa ttccgtggcg cacgctgtcg ggacgccagc agctctatca
     4861 ggatcatccg tggatgcgtg cttttggtga aagcctggtg gcttatcgcc cgcctatcga
     4921 cacccgtagc gtcagtgaga tgcgccagat accgccaaac ggcttccagg aaaaagcact
     4981 taacttcctg acgccgcacc agaaatgggg cattcactca acctacagtg aaaacctgct
     5041 aatgctgacg ctctctcgcg gtggaccgat tgtctggatc agcgaaacag atgcccgaga
     5101 actgaccatt gtcgataacg actgggtgga agtatttaac gccaacggcg cgctgactgc
     5161 ccgcgcggtg gtcagccaac gtgtaccgcc gggcatgacc atgatgtatc acgcccagga
     5221 acgcattatg aatattcctg gttcggaagt aactggcatg cgcggcggca ttcataactc
     5281 ggtcacccgc gtttgcccga aaccgacgca tatgattggc ggttacgcgc agctggcctg
     5341 gggctttaac tactacggca ccgtcggctc gaaccgcgac gaattcatca tgatccgcaa
     5401 gatgaagaac gttaactggc tggatgatga aggtcgcgat caggtacagg aggcgaaaaa
     5461 atgaaaatac gttcgcaagt cggcatggtg cttaacctcg ataaatgtat cggctgccat
     5521 acctgttcgg tgacctgtaa caacgtctgg accggacgcg aaggcatgga gtacgcatgg
     5581 tttaacaacg tcgaaaccaa accgggcatt ggttatccga aaaactggga agatcaggaa
     5641 gagtggcaag gcggctgggt ccgcgatgtg aatggcaaga tccgtccgcg tctgggcagc
     5701 aagatgggcg taataaccaa aatcttcgcc aacccggtgg tgccgcagca gattgatgat
     5761 tactacgaac ctttcacctt cgactacgaa catttgcata gcacaccgga aggcaaacat
     5821 atccctactg ctcgcccgcg ttcactgatt gacggcaaac ggatggacaa agtgatctgg
     5881 ggcccaaact gggaagaact gctgggcggc gagtttgaaa aacgtgcccg cgaccgcaac
     5941 ttcgaggcca tgcaaaagga gatgtacggg cagtttgaaa acaccttcat gatgtacctg
     6001 ccgcgcctgt gcgaacactg cctcaatccc agctgcgtgg cgacctgccc aagcggcgct
     6061 atctacaaac gcgaagaaga cggcattgtg ctgattgatc aggataaatg ccgtggctgg
     6121 cgtttgtgca ttagcggttg tccgtacaaa aaaatctact tcaactggaa aagcggcaag
     6181 tcagaaaaat gtatcttctg ttatccgcga attgagtcag gccaacccac cgtgtgctca
     6241 gaaacctgcg tgggtcgcat ccggtatctg ggcgtgctgc tttacgacgc cgaccgcatc
     6301 gaggaagcgt cgagcaccga gcgcgaagtt gacctc
//
LOCUS       DAEEUE010000159         6332 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.159, whole genome shotgun sequence.
ACCESSION   DAEEUE010000159 DAEEUE010000000
VERSION     DAEEUE010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6332
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.159"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            5..436
                     /locus_tag="KLK84_003801"
     CDS             5..436
                     /locus_tag="KLK84_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HBE2010945.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="KLK84_003802"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="KLK84_003802"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HBE2010946.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="KLK84_003803"
     CDS             complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="KLK84_003803"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HBE2010947.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="KLK84_003804"
     CDS             complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="KLK84_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBE2010948.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4438..5145)
                     /locus_tag="KLK84_003805"
     CDS             complement(4438..5145)
                     /locus_tag="KLK84_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HBE2010949.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="KLK84_003806"
     CDS             complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="KLK84_003806"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBE2010950.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6003..6206)
                     /locus_tag="KLK84_003807"
     CDS             complement(6003..6206)
                     /locus_tag="KLK84_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBE2010951.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
BASE COUNT         1406 a         1810 c         1648 g         1468 t
ORIGIN      
        1 gaaggtggcg ttaaatctcg cttcaggtga agggaatttc tttatccgtc ctggcggcgt
       61 gttttatgtc gcgggagata aagtcggcat cgttcgtctg gatgctttta aagccagtaa
      121 agatattcag tttgcggtgc agtcaggacc aatgttgatg gaaaacggtg taattaatcc
      181 gcgtattcat cccaacgtcg cctcacgtaa aattcgtaac ggtgttggga ttaataaaca
      241 tgggaatgcc gtgtttttgt tgagccagca ggcgacaaat ttttatgatt ttgcctgtta
      301 tgccaaagcg aaactgaacg ttgagcagct gctttatctt ggtggcacga tttcacatat
      361 gtatatgaaa ggcggggcaa tcccgtggca acgctatcct tttgtcacca tgatttccgt
      421 cgagcggaaa ggttagaatg atagctttgt gtcagatact aaatatgctg ctgatgccgg
      481 atgcggcgta aacgccttat ccggcctaca tgacgttgca atttattgaa tctgaatgat
      541 tttttaggcc aaataaggtg cgcagcaccg catccggcaa cgttacaccg actccagccg
      601 ggcgtaagct gccaccagcc atttgattcc ctggccctgg aatgccacct gcaaacggct
      661 atgttcaccg ctgccttcca tattgacgat ggtgccttca ccaaacttag cgtggcgtac
      721 acgctggccg agcttgtagc cgctgtcgtt ctcgaccatc ggcgtaccca tccgctgatg
      781 gctgaccggg cggcttaccg tggcgcgcag gcgcacctct tccacacact cttccggcaa
      841 ctcgccaata aagcgcgacg ggcgatggta aacctctttg ccatacagac ggcgagtttc
      901 cgcgtaggtc agcgtcagtt tctgcatcgc gcgggttacg ccaacgtagg ccagacggcg
      961 ttcttcttcc agacgcccgc cttcatccag cgacatctgg cttgggaaca tgccctcttc
     1021 cataccgacg ataaacacct gcgggaactc cagacctttc gccgagtgca tggtcatcag
     1081 ttgtaccgca tcctgccagg tatccgcctg cccttcgcct gcttccagcg ccgcatggga
     1141 taggaacgcc tgcagcggca ttaaatcttc gtcttcttcg ttgtagctga actggcgcgt
     1201 tgccgtcacc agttcctcta agttttcgat acgcgtctga cctttttcgc ccttctcctg
     1261 ctcgtacatg gtacgcaggc cggagtcttt aattacccgg tcagtctgta catgcagcgg
     1321 catatcggca gtttcctgcg ctaaggcgtc gatcagttcc ataaaccgct gtaaggcgct
     1381 ggcagcacgt ccggcgaggg ctttttcctg caacagttca cgacatgcct gccagagtgt
     1441 taactggcga tcgcgcgatg tctgacgtac cacgtccagc gtccggtcac caataccccg
     1501 cgttggcgta ttcaccacac gctcaaaggc cgcgtcgtcg ttacggttgg caatcaggcg
     1561 cagatacgag agcgcatctt tgatttcctg gcgttcgaag aagcgcatcc caccgtaaat
     1621 acggtacggc atactcgcct gcaacagtgc ttcttccagc acacgcgact gggcgttact
     1681 gcggtagaga atggcgcact cggcaagcgc cccgccgttg tcctgccagg ttttgatgcg
     1741 gttaaccaca aaacgcgctt catcgagttc gttaaaagcg caatagaggg aaataggctc
     1801 accgtccgcg ccatcggtcc acagtttttt acccagacgc ccgttattgt tttcaatcag
     1861 ggcgttagcg gcgctcagaa tattgctggt agagcggtag ttttgctcca gacgaataat
     1921 ttcggcaccg gggaaatcat taaggaaacg ctgaatattc tccacctgcg ccccgcgcca
     1981 gccgtagatt gactggtcgt catcaccgac gatcatcact ttgccggtgt cgcccgccag
     2041 caggcggatc cacgcgtact gaatgttgtt ggtatcctgg aattcgtcca ccaggatatt
     2101 ggtaaaacgt tcgcggtagt gttgtaggat atgcggcttg ttaagccaca gttcgtgagc
     2161 gcgcagcagc agctcggcga agtccaccag gcccgcgcga tcgcacgctt cctgatacgc
     2221 ctgatacacc ttctgccagg tctgctccac cggattaccg tagctttgaa tatgatgcgg
     2281 acgcaggcct tcatctttct ggctgttgat gtaccacatt gcctggcgcg gcggccactg
     2341 cttctcgtcg aggttcatgg ctttgatcag acgcttaagc aggcgtagct ggtcttcgct
     2401 atcgaggatc tggaaatcct gcggcagatt ggcgtccata tggtgcgcac gcagcaggcg
     2461 gtgcgccagc ccgtggaagg tgcctaccca catgccgccc tggctggtgc ccatcagttg
     2521 tccgatacga tgacgcatct ccgccgccgc tttgttggta aacgtcacag ccataatcga
     2581 gtatggcgag cagttttcca cgctcatcag ccaggcgata cgatgcacca gtacgcgcgt
     2641 cttaccactg cccgcgcccg ccagcaccag aaggttgctg cgtggcgcgg ccaccgcttc
     2701 gcgttgtttg tcattaaggc tgtcgagcag gtaagaaacg tccattggca ccgccgcgta
     2761 aaaataggtt gggcggagaa gcgcaacgcc ctccgccaaa aagctgggta tatatacaga
     2821 tttgctgatt atatcagcga ggtcagagat gccaaccggg aaatttccag atgtggcagt
     2881 aaacggctgt cccaggtttg catcagatcg ccattttccg gtctgatcca acaagcctgc
     2941 attccgctgc gaattgcccc acccacgtca gtggtgagat cgtccccaac atgtaagatc
     3001 tcgccgatcg gcacgttgag tttttccgca gccaaaaagt acatatcgct gaacggtttt
     3061 gagcgcccgt gcgggccagc gcgcagcaca aactcaaaat aatcccccaa accaaacagt
     3121 tccggctggg cgttaccgtt ggtgatcgcc accagcggcc atttcttcgc cagctgtttt
     3181 aaggtgtcgt gagtttgctg cgggacgtcg attcggctgc gccatttggc aaagttaatc
     3241 attgctgcgt gtgcgcctgc actggcttct tcggcactca gcccggcgtc gagcatcgct
     3301 tgttcaatcg aacgaaaacg ccaacgtgtc acgtcgtgat aaatctcggg ttccgcttcc
     3361 cgtaccgcct ggcgcaggcg ttgcagatct tcattctgga agctgcgcag cgccggatga
     3421 taattttgca caaaggtaag cgcctctcgc tcggtgcgca aaatcaccgg acggttatcg
     3481 taaagggtat catccaggtc aaaggtgagc gccgagatgc gccccaaagg ccggtaaaaa
     3541 cgcattattt cccccgtttg gcgcgtggat gcgccgcatc gtacaccgag gcaaggtgtt
     3601 gaaaatcaag atgagtatag atttgcgtgg tggagaggtt ggcatgaccc agtagctcct
     3661 gcacaccacg aagatcgccg ctcgactcca gcatatgcgt agcgaacgag tgacgtaatt
     3721 tatgtgggtg aacgtgatta ttcagccctt gttttatgcc ccattcggca aagcgtttct
     3781 gcacattacg cgcggagata cgcttgccca gtttcgacag aaaaagcgcg tcgtcttcgc
     3841 taccaaacag gtcgcgcaaa tcaagccagt gctcaatcca cgccacagcg ttgcgaccaa
     3901 tcggcaggcg gcgctctttg ctgcctttcc ccatcaccca cacttcgccg gactccaggt
     3961 cgaggtgttt gatatccagc cccaccagct cggaaagacg cagacccgcg ccgtacatca
     4021 cttccagcat tgcacggtcg cgtacagcga ggggatcgtt gatatcaata tccagcagcc
     4081 gattcatatc gtcgacgtcg atatttttcg gcagatgacg cggcgctttc ggtgccgaaa
     4141 ctcctttcgc cgggttagct ttgagttcgt tctggctgac cagccagtca aaaaagctac
     4201 gtagcgcaga aagccgtaac gccagacttg ctgctcccag ccctttacgg cgactgcgta
     4261 cagcaaaatt gcgtaccatc gctgcatcgc attgttgcca gctttgcagg ccgttttcgc
     4321 tggcaaaatt gatgatcgcc tcaagctgac gctggtagtt aagtagggtt atcgggctaa
     4381 gctgacgctc cacgctcaga taacgtaggt agcgttctac atcggtgtgt aaatcggtca
     4441 tacgcgttca atccaacgct ccagaagctc cggcaacatc agcgcaattt catgaagtaa
     4501 ctgcgttccc tgcccttgtt gatagtgact ggcatcgcga ctggtaaaca gcacaacacc
     4561 caaatcagca tcgcttccca gcatcgacat cgccaccgat cccaccgctt tcgcttccgg
     4621 tagcaccacc agcagctccg gtccgttaag cggcccaaga tagtgctgtt cctgccccaa
     4681 acgctgaata cgcagcggtt cgaaagactg acggcttaat gccagatgag tgtggttcga
     4741 cggcgcacct aagcgccagc gatctggaaa caggcgcaga ctcgcaccag ctaggccgag
     4801 atcgcgcgcc cagcggtgaa agcgcatcag catatcgtcg agactgctgg cggcggtgag
     4861 actgcgctgc aggtagagta ggcgataaaa cagcccttcg ttggcgatag cctgctccat
     4921 caacaacgcc atgttctctt ccagaacatg aatgtgatta cgtgcgcggg ccatgtgcca
     4981 ctcgaccaac gaaacggtgc cgcgtaccgg atgcggcaca cgtatcgctt ctactgcgcg
     5041 cgcattgcgg ataaaaaact caggattttt aatcagataa tcgacaaccg cccggtcatc
     5101 aagctccgtg agtgtttcct gcagttcttc ccctggttgc ttcatagatg aataaatcca
     5161 tcgtagacat gtaccgccgg gccagtcata tataacgggt gacccggacc tttccaggcg
     5221 atatcaagac gaccgccggg gagttccacg cgtacttctt cgtccagcaa accttgctga
     5281 atccctaccg ccaccgccgc acacgcgccg ctgccgcagg cctgggtttc tcctgcccca
     5341 cgctcataaa cgcgtaaacg aatatgctcg cgcttaacca cttgcataaa accgatattg
     5401 gcgcgctccg gaaaacgctc gtgactttcc agaacaggac caagcgtttc taccgccgcg
     5461 gtatcgacat catcgacctg aatcacgcaa tgcggatttc ccatcgacac cacgccgcat
     5521 aagattgtct gctcggcggc gcgcataata taggtctttt ccgctttgtt agcgcgaaac
     5581 ggcacggcgg aaggttcgaa gttgggttcg cccatattta cgcggaccag atcatcatcg
     5641 gtgacggtca gaaccatccg cccgttggcg gtgctgacgc ggatatcacg cttattggtc
     5701 agtcctttca gacgcacaaa acgggcaaag cagcgcgcac cgttaccgca ctgcgccact
     5761 tcactgccat cagcattgaa aatgcgatag tgaaaatcca gttcaggatc atacggcggc
     5821 tcaaccacca gcagctggtc aaaccctacc cccaggtgcc gatccgccag gcgacgaatc
     5881 agctccggtg aaaaaaagac attctgcgtt accgcgtcga cgaccataaa atcgttgcca
     5941 aggccatgca ttttcgagaa ctgcatcatt tactccaatc acgcgggtac agaaactgac
     6001 ttttagtaat tcacctggga tggaccatcg ccagtggcgc gatcgttttt atccggcacc
     6061 gtggattgcg tttgcgtctc taccggtttg gtcggcggcg gtgcgttttt atctgcaggc
     6121 gggaaataga gcggaccttt cagaccgcag cccgtcaggc tgaagagagt aagtaataca
     6181 gtgagtgcct taaacacgtt tttcattatg gattgcctgt aaagttcatc gctttctgct
     6241 ttctatcatc gcaggagaag gcgtaaaagc aagcgtttag cgactcttta tgaagtctga
     6301 aaagtgagga aatgctgcga gtataacgct ta
//
LOCUS       DAEEUE010000160         6268 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.160, whole genome shotgun sequence.
ACCESSION   DAEEUE010000160 DAEEUE010000000
VERSION     DAEEUE010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.160"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..666)
                     /locus_tag="KLK84_003808"
     CDS             complement(<1..666)
                     /locus_tag="KLK84_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphorylceramide synthase"
                     /protein_id="HBE2010952.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIK
                     IALP"
     gene            complement(656..2413)
                     /locus_tag="KLK84_003809"
     CDS             complement(656..2413)
                     /locus_tag="KLK84_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HBE2010953.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAVSDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRNAGFDKCT
                     QRIDVWGIFTVSMAVRRDN"
     gene            complement(2429..3313)
                     /locus_tag="KLK84_003810"
     CDS             complement(2429..3313)
                     /locus_tag="KLK84_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBE2010954.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(3324..3929)
                     /locus_tag="KLK84_003811"
     CDS             complement(3324..3929)
                     /locus_tag="KLK84_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBE2010955.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILFICTAINRCRSVLLMSAER"
     gene            complement(4100..>6268)
                     /locus_tag="KLK84_003812"
     CDS             complement(4100..>6268)
                     /locus_tag="KLK84_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HBE2010956.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1835 a         1568 c         1316 g         1549 t
ORIGIN      
        1 tggcagtgct attttgattc gacgttgatc aagtttctga taattccaac gacggtcgac
       61 gggcaccatc cagtcaatca acatacctac cgccagccct gtgatgacat caataaaatg
      121 atgctgccat gtcgtcagcg tcgaaatggc gatgagtaaa aaccatccgc cacagacttt
      181 acgccacctc acagccagat gctgacgaaa gtgacgccaa agtagccagc agagaataat
      241 atgcagcgaa ggagactggt tataaggcag atcaaacagt tcaagttgcg aaaatagcca
      301 tcctgtcacc ccactcactt caggacggat aaaactaaac ttcagcgggt agagaaaaaa
      361 accgcagcag gccattaccg ttgccagaat aagccggtgg acaagtcggc gttgttcgaa
      421 tgtcgagcta caaacgaaca gcgaaaatcc atataaaaga tccagactcc agtaaggaac
      481 aatagtccag ggaagaaaag ggatcgccgt ttcccaaccg aatacctgac tggggatatc
      541 atggctgtta aggtcctgaa ccgcggtgaa ctgattaaga gatccatagg tgaagaagaa
      601 aaacggggcc agcaacaata accagccagc gccttgtagc aatacgttcc gacgttcagt
      661 tatcacgacg caccgccatc gaaaccgtaa aaatgcccca cacatcaatc cgttgagtgc
      721 atttatcaaa tccggcatta cgcacgagcg aatccatctc cccttgcgaa cgcacgcgca
      781 ttacccacgg tttaccatct ttatgactgg ttaacacccc ggcaatcagc tccagttgtg
      841 ggtgccacgg ctgcccggtg tagatgagaa tgccacccgg ttcgatggca ttggctagac
      901 ctgcgagtga gttttttacc tgctcatttt cgggaaaaag ctcatatagg ccagagacaa
      961 tcgccagcgt aggccgcgga gttaacgcgc tgagttcctc cgggttaaac gcatcgccct
     1021 gttcaaaacg cacccgccca gacattcccc gttgggcaat catctcttgc ccctgtgcaa
     1081 catttaactc actgtaatca cgtaacaaaa tatcgcttac ggcaggctcg tttgccagcg
     1141 catccagtac atagcgtcca tgccctgcgg caatgtcaac cacgcggacg gctaaacctt
     1201 tggcgtggag atcggcaacg gcttgtttaa tcaatatttg caaatgggtt ttgcgctggc
     1261 gaataccgcg ccatccaaca ctgttcaggt agattttgtc gattaaacgc ccgaatgcgt
     1321 tactgccttg cggttgattt tgatagacat aatccaggga actgcctgaa tcaaagccgg
     1381 tactcattcc gagatgcagg cccgcagagt gcgcaccgaa caatttcatc gcctttcgca
     1441 taaagcgata cgctaaatca accggcgata atggcacggg tccaccagaa aggagccgcc
     1501 atcgatccgc tgatggtcct gtgcggtctt catgctgata atcaaatttt tgcgatttgt
     1561 tagcatataa gcggcagata aagctttgca ttttttcaaa tgcctgcgcc ctgttctctt
     1621 cacccaatgt gtcgtgataa aagcctggca gcagatgcag ctctttcaga gggctacgta
     1681 atctctgata aaaatcaatc tgcggttggc gatgcaccac atagtcatca cctgatatca
     1741 gaagttgcgt ggggagcgta atcgccgccg catcgctaac aatacgttca gacgttttat
     1801 agagatcgag caagatattg acggcaatcg cccgtgtgat cagcggatca ttattgaaac
     1861 tcgccacccg ttgccgatcg tgggtcaaat agcgcccttt cacataggaa ttaataaaaa
     1921 atagaccacg cagacgatgc cataacgcca gcgcaggacg tgccagcggc acatacaatt
     1981 taaccttaaa ggccggagaa gccagcacca gcccacgaat tgcaggtgca taatcatgaa
     2041 cccatgtggc aacaagcact gcgccgacgc tttgcgcgat cactaccacc tcttccagtc
     2101 cgacctggct gtcgctgaca gcaaaacgga caaattcatc gacatcctgc actgagcgca
     2161 taagagacgg gctataacca cgcggccccg aattttttcc atgcccccgg gcatcccagg
     2221 cataaaaagc agtatcaggc atcgccagtt catcaacgag atgttgtaga cgaccagaat
     2281 gttcatgccc gcgatgaaat aagacgatga cctttttcgc ccctggctgt aaagtgggcc
     2341 agtgccgata atacaacgct gtattatcac tggtggtaaa aaaatgttcc ccagggatgc
     2401 gtgaattttc cattttctct attttccttc agtaacagca gtagcgaata aaataaaaaa
     2461 ataccggagc ggtgaaaatt aatgagtcaa tcctgtcaag gagtccgcca tgtcctagta
     2521 atagttttcc actatcttta acaccaatat ctcgtttgat ggctgacatc actacatcgc
     2581 cgcaaaaacc actaatacca attaacaaac ccgctaataa tgcctgtaat gtatttagcg
     2641 gtgtcagcaa cggtccaata atcagtgacg caatcatggt ggtgattacg ccccccagca
     2701 gaccttccag cgtttttccc gggctgactt tggggaccac ttttcttctg ccgcaggatt
     2761 tcccccataa atactgtgca atatcgtttg actcggttaa cgccaaaaga aataacacca
     2821 gtaacgcccc gccctggata tttatggttg gcaacattaa tagccaggcg gcatgactca
     2881 aggcaaaaac ggtcattatc catccccaaa aaatggcgct cacggtatgc tgaaaaccgg
     2941 aggggtcacc cacaaaaact cgccaggtgg ctaatatcag aaacccagtc aaaggtataa
     3001 ataacagaaa cagctcaaag caattaaaac cgataagcaa atagttaaga ggaataaccc
     3061 aatataacca acgcgaaaaa tgtacggata taagcgtaca atattctttc aatgccagaa
     3121 aacttatcag cgtaaaaaac gtcaacgtca tccagcgtgg gcctgaaatc accagtgaaa
     3181 aacaaatgac gatgccccac caggtacgaa tacgtaatgt aacttcatcc ccttttctct
     3241 caggtaaccg ccatagcaga aaacggttta tcagcttgct aaaattaaca acgcgaaaag
     3301 tgttgccaga gatttttcca gcattatctt tcagcgctca taagaagcac actccgacaa
     3361 cgattaatcg cagtacagat aaacagaatt gaagcaatgc tccataaaag attattccat
     3421 tgcatccact gtggataaat ggcaacggcc agaccccaca gaccaaatat taacgcgcga
     3481 tcacttttgc caaatggacc ggcataactg cgaacaccgt taatcgtctg cgcgagtaaa
     3541 ccgcaaaact cggtcaatat ggtgcaaaat aacatgagta tcacgagtga tgcgttactt
     3601 tccggtaaaa ataaaaacgg taaatagagc gcaatatcgg aaataacatc gccagtctca
     3661 ttcaaaatcg cccccagacg tgtttgctga ttgcactcac gcgccaacat gccatccagc
     3721 gcattgagcg ccatacggat aaaaagcacg atgggcaata gcaaaaagag gatgggttgt
     3781 gccaccaaca ccaacagcaa tccggtaaaa agagaaagcg ccagtgccgt aagagtgatg
     3841 tgattcgctg taacgtggtg cttataaagc caaaacatcg tcggccttaa cagcgactga
     3901 aagagcggtt ttatctggta gagcgtcatt atcaatcctt gataattcgg tttgcgctat
     3961 ggtgcatcaa ttcccctgac ggagatatcg caacacgccg cttcattaaa aattcagaat
     4021 atatcaaggt attgggggct cactgtcagg aatgattatc cccaataaat caagtagtta
     4081 aaaattatta cgctgttatt caatacaact catcaagttt cctcatcgct tgttcccgta
     4141 ttgcgcgcgt cttaccgtta agcgccagct ggcggactaa atccagttca gggttaacaa
     4201 gctctaacgc cagcaaagga tcacgtttca gcctcgcctt caatacatca actggaaagc
     4261 ggggattatt catcgccaca atccaccact cgggatcaat ttctgccgcc agtgccgcag
     4321 agggggtgtg gcggttgcta agtgcgccga acttgaaaaa tgtgagattg cttttcgcca
     4381 gtgtactcca ccatatacga tccggttgtt ttgccagtac atccagttgt gctaacgcat
     4441 cggggtcgag tgcataataa acacgtaatg cttcatcaac ctgcgcacgg gagctatttt
     4501 cctgcctcag aaaaaacttc gcccaaatat cattgcgtgg cgtgttttgc tgtaattcct
     4561 tgagaacaac agcgggtaaa ttatcccaac taactaatgc actctctaaa aagtcccagt
     4621 cttccaaacg tatcagggcg gcggcattta atagcatctg gtcatcaact tcgccgtggc
     4681 taatcagctc agacaaaatc atatcagcgc tggtttcata tgcatctgaa tctgctttcg
     4741 gattattttt ccgatgcgat aaagcacgtt gccaaatttt ttgtttcatc gacgtgctca
     4801 gatactgatt tttcgccaga ttactccacc cctcaggatc gttgaaacgt gtcaataacc
     4861 gttgtaaagc agcgtcagag tgaattacct gcatatcccg acagcaaaag taggcagggg
     4921 attttttctc catcctttta acaaaattaa actgtaagtt atctggcaat gctaaaaata
     4981 acacacgtat tagatcatcg tcattctcga atttttggta taaataggct gcaactttgt
     5041 cactctcttg tggcgtcatc cgcaataatt gaccatggtg tttcatctct gccagcttga
     5101 acgctaagct tgtgacaaca ttgtttgatg aatcgttcgc caactgcatt gcctgattgt
     5161 aagtgagttg tcctccagca ccgacctgac gaatagagga atcatcactg cggatatagc
     5221 catcaataat ggattcgggc aataaccacg catctttata aagctcctga atgatcatat
     5281 cccaatctct tttcgccaga gtcatgacga tatccagagg gagattttca cgtagcgcaa
     5341 gattcaacgg accgacacca tcaggtagac gactcattcg cgtcattatt tcgacagaga
     5401 gcggttcacg gtcgagcaac cccagaacaa caggctggta acccaattgc tgatcggtca
     5461 actccagaat ctcattgtca ggcatatatc tcgccatact ttcaatcgtt tgttcatcat
     5521 tgctattaat aagttgtttg cgtaggttag ctgaaattcc tttaagcctt gggagagttc
     5581 gcattatggc aagatcaaat gtcgcaatat ctcgtccttc aaatgcagca acgttgcctt
     5641 catccagcat ttgcaattcc tgcactaact caggagaagg cttagggcga aacagcgctt
     5701 cttttttcat ctgattacag acattccctg ccatgcgaat attgcttcgc ttaaaaattt
     5761 ctttcagagc atcttcatca tttattagac cccaataata tgcatcagga tttattatca
     5821 tcagcgcttt tttgacattt tcagtgtcat ttttgtcact cgcacggacc atgaattcac
     5881 tatttgtcga agacgcaaca acaggtgcaa ttatgtcatt gataaatacc ccacgcgcat
     5941 taatactccc cttaatcgta gacggattat cgtaatatga gtcgataaat accagtccac
     6001 ttgcatttat cgaaccggta attaacagtg gtgaatccgt aactatattc gttgccgtca
     6061 cgttaccgag aataacccat ggattagaaa gagagaaagt ggaaatatta cctttgactg
     6121 tcaaattgcc aagaacaatc aatttttgat tactctctat attaccttct accactaaat
     6181 cgccacgatg cactttccag tccgatgcag ataaaccgaa ggggacaaga ttcacgctac
     6241 tgttgatagc ttgttcaact tgttgttt
//
LOCUS       DAEEUE010000161         6252 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.161, whole genome shotgun sequence.
ACCESSION   DAEEUE010000161 DAEEUE010000000
VERSION     DAEEUE010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.161"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="KLK84_003813"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="KLK84_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HBE2010957.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="KLK84_003814"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="KLK84_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HBE2010958.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="KLK84_003815"
                     /pseudo
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="KLK84_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5797..6054)
                     /locus_tag="KLK84_003816"
                     /pseudo
     CDS             complement(5797..6054)
                     /locus_tag="KLK84_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1656 a         1456 c         1476 g         1664 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attatcttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt ttaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgccta ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgtggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagataaat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atataaagcg
     6061 cgcaaaatta ttcaagatat ttttgaaaat cattcaagat aggctatcgc gtgtgatagc
     6121 aaaaacattc aagataaaaa tgcttgatta ttaagcaatc aggaatggga gtattaagtt
     6181 tcgtgtttcc agtaaaacca gagagtattg aaatagctat cacctaactt tatcaggcga
     6241 tagctggtag at
//
LOCUS       DAEEUE010000162         6252 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.162, whole genome shotgun sequence.
ACCESSION   DAEEUE010000162 DAEEUE010000000
VERSION     DAEEUE010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.162"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(53..532)
                     /gene="xdhC"
                     /locus_tag="KLK84_003817"
     CDS             complement(53..532)
                     /gene="xdhC"
                     /locus_tag="KLK84_003817"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HBE2010959.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(529..1407)
                     /gene="xdhB"
                     /locus_tag="KLK84_003818"
     CDS             complement(529..1407)
                     /gene="xdhB"
                     /locus_tag="KLK84_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HBE2010960.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1418..3715)
                     /gene="xdhA"
                     /locus_tag="KLK84_003819"
     CDS             complement(1418..3715)
                     /gene="xdhA"
                     /locus_tag="KLK84_003819"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HBE2010961.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIDWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            4130..4885
                     /locus_tag="KLK84_003820"
     CDS             4130..4885
                     /locus_tag="KLK84_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBE2010962.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4964..5037
                     /locus_tag="KLK84_003821"
     tRNA            4964..5037
                     /locus_tag="KLK84_003821"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4996..4998,aa:Gly,seq:ccc)
     gene            5152..>6252
                     /locus_tag="KLK84_003822"
     CDS             5152..>6252
                     /locus_tag="KLK84_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBE2010963.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSVSLRT
                     KDRRTAMAHSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDR
                     FNDIDRQDDQADSVSLSVSAPEVKPEVTPSITIASLFEQYEAENSQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLKVRDVLQQLPKNRKQRFKDAPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLN"
BASE COUNT         1554 a         1569 c         1619 g         1510 t
ORIGIN      
        1 gttaaacaca taacctcagt gaatccggcc tgaattcagg ccggatatcc ttttacttcg
       61 ttttctcgca atccagaact gtatttacaa tcatctgata ccccgtgcag cgacaaagat
      121 ttcccgccag tccgcgacga atttccgtaa tggttaatgg cttctcgcgt ggtttcgcca
      181 gcattgccgt ggtagccata atcaggccag gcgtacaaaa cccgcactgc actgtgccgg
      241 atttcgcata agcctgctga acatgagaaa gttttccgcc tttcgcttca ccttccagcg
      301 tgcggatctc ttttccttca gcccaggcgg caaggtataa gcaactgtct attgctgtgc
      361 cgtcgaccaa caccgtacag gcaccacatt cacccacgca gcacccttgt ttgacactta
      421 gcagtccttg ttcgcggagt aattccgaga gcggcgtgcc tggtgcggcg tgaagctgaa
      481 aaggcatccc gttaatggtg cattcggtgg taattgtttc gctgtgattc attgcaattt
      541 tccccccgcc gcggcgacgg cttcgctaat cacttttttg gtcatcgtct ggatgagatg
      601 cagacgaaac tctttactgg cccgccatga agaacgcggg gcgacatctt gcaggacaga
      661 ttcgctgata gcttccagcg tttgcaggtt taatggcgca ttttgtgcag tctgttcggc
      721 atgttggcag cgaatcggcg ttggcgcggc aacaccaaat gccaggcgta attcgctgaa
      781 attgccgtta tccagtcggc aatgtgcggc gcagccaatc gttgaaatat ccattgcgtc
      841 gcgcatggca tatttaaaat gcgcgctgcc cgcgtgttct ttcggctgtg gcggaaaatg
      901 aaaggcgacg aggatttcgt catgctcaag agacactttg cccggcccgg tgtgaaagcc
      961 attaatcggg acgaaacgaa caccgcgcgg ggagtggatc tccagtttcg cgtcataaat
     1021 tagcgttggc gtggcagaat ctgcgctggt ggcaccgttg caaatatttc caccgtaggt
     1081 agcgacgtta cggatctgcg gtccagcaat gaacgtggcc gcagcacata acgccgggag
     1141 atgacgttga gttataggat cttctattag ctgggtaaat gtcgttgcag agccgatacg
     1201 tagcgagcca tcttccgcca gcgtaattcc ccgcagctcc gccagattat gaatatcaac
     1261 aatatggcga taacggtcat tgtgatggtg gagctgaatc agtacgtcag tgccaccggc
     1321 gagcagtttg gcctgcgggt tgtcagccag caggttgatg gcatcggcaa gggttgctgc
     1381 gcgatggtaa gaagcaaaat caaacatgat gttatcctca aatcaatcct gccagatgga
     1441 actcttcata taaccgtttt ggcgtcagcg gcagtgtatt gattgcaaca ccggtagcca
     1501 tcttcaccgc gttacgaata gcagcggcaa caggaattat gggtggctca cccagtgact
     1561 tatgtccgta tgcggattgc ggctcattga tttcgacgaa cgcgctttcc agttgtggca
     1621 gatccggcat ggtcggcatt ttgtaatcca gcagattggg gttacggacc acgccgcttt
     1681 tagcatcgat gatcatctct tcaaatagcg cccagtcaat gcccattccc attccgccgt
     1741 gtacctgacc ttctgccagc agtggattaa gaatatgccc tgaatcatga acgttgagga
     1801 tgcggttgat ggtgactttg cacagcgcaa tatcgaccgt cagatcaaca aaggtacagc
     1861 caaacgccgg tgggttagtg gtggttttga tggagctttc agcagagagc tgcccgccgc
     1921 gttcagggtg gtagaaagcg tccatcgcca aatcttttaa cgacattaac ggctcttccg
     1981 gtcgttcaac cagcacgata tggcctttta tcagggtcaa attcatcgct gactgatgta
     2041 gcatgactgc ggcgtgagcg atgattttct cttttaataa tagtgccgca ctgcgcagcg
     2101 caggcgcggc aacatagctc tggcgtgagg caaatgcgcc gggatcgaac ggcgtaacgt
     2161 cggtatcttg tgttgaaata acgcgaacgt cgctgaccgg aacccccacg gtttctgcca
     2221 ccatttgcga gaagacggtg tcggcaccct gaccgatttc cgtcgcgccg ctttgcacgt
     2281 tgatggttcc atcctgattc atcagaaggc gcgcgcctgc tatttctacg ccgacaggcc
     2341 aggtgttaga ggtgtagcta aaacaggcga cgccaacgcc gcggcgcaaa ttgccttgct
     2401 ggttctggca ttctgcacgg cgtttttccc attcaaagat tttccggcct ttttcaagac
     2461 actccggcaa ccctgcgctg caaatacgtt tgcccgtgag cggattagca tctccttcgc
     2521 gggcggcgtt gcgtaaacga atttcaacag gatcaatacc taacgctgtc gcggcgtcat
     2581 caagcataga ctcaacggca aatacgactt gtggcgcgcc ataaccacgc atcgcaccag
     2641 ccgaggggag gttggtatag caggtctttg aactgtaagc gtaggcacaa cgaggataaa
     2701 ggtaagcgac tttattcccc cccgcagaag cgatggagtg cccgtgagat gcataagcgc
     2761 cggtgttaga cagaacatcc agactataac ctttcaaggt tccgtcgcgg ttcacgccca
     2821 tttgcccgtc aatagtaaaa gcgtggcggg tacgggttgc gaggaaacac tcttcacggc
     2881 taagggaaac tttcaccgga atgccgccaa gcttgctggt caggaatgcc gccattggct
     2941 cttccagtac atcctgttta ttaccaaaac cgccaccgac aaacggtttg atgactcgta
     3001 cgcatgacca gggaatatcc agcgcctgac caaccacgcg gcgaacaatg tgcgggatct
     3061 gggtgctgga aacgatggta attcgcgagt catcctccat ccacgccagc gatgttacgc
     3121 tttccatgtg acaatgttga ataacgggag tctgatagtg cccctgtacc tggtagtcgg
     3181 cggcatcgat tgtttgttgg acattgcccg tcgacatcgt gctttgtttc agtaaattgc
     3241 cgccgttatg gattggtgca gcgtcttctg tcagcgccgc ttctggcgtg gtgataacgg
     3301 gtaattcttg ccactcaatg ctgaccaatt gcgccgcttt ttctgccgtg agttcatcgc
     3361 gggccacgac gatggcaacg gcgtcaccat gatgacgaac atggcgagtt agcagtgcgc
     3421 gatcggcggt atcgcgcttg ttttcgtcaa gtgtccaggc atgcccagct gtagcgaatg
     3481 gaatatcagg cacatcctcc caagtaaaaa tcgccagtac gcccggtaaa cttctggctt
     3541 gttcatcatt aatacttacg gcataaccat gtgcgatagg gctacgtaca tatttcgcgt
     3601 aacacatgcc cgccataaca taatcgtcag tatatcgtgc ccgcccggtg accttagcaa
     3661 tggcatcgac gcgcatgcat gattcacccg tagcggttgc ttcccgcgct tccatagaaa
     3721 tcccctcgat ttgaacattt ggttcttatg gcaaagattt agtctaagca ggcaagattt
     3781 acgccagaaa cgacgataag cgttagggga ggaaggaagt gtgatgacgt taaaaaaaca
     3841 cagatatgat acgaaacagg actctgaata gggacagtga tacgtatcaa tatgagtggc
     3901 gataaaatca atgtgataaa tcaggattat tacgcatcag gatgggaaat agggtaaaaa
     3961 gtcaggaact tcgatacctc tacagcgaat attttgctga tgatacttcc ctttgccaaa
     4021 tgaaattaac ctctttctct attaccacgt ttttccagaa gcaagagatt gggtatcaag
     4081 aggctggctg ctatgatatg gcgtcttgtt ttttaagcga ggaaagattt tgagtgcggg
     4141 acgcctgaat aaaaaatctc tgggtatcgt gatgttttta tcggttggac tgctattggc
     4201 gggctgttcg ggtagcaaat catccgatac aggaacgtat tccggctccg tttacaccgt
     4261 gaaacggggg gatacgctat atcgtatttc gcgcaccacg ggaaccagcg taaaagagct
     4321 ggcgcgactg aacggcattt ccccccctta caccattgaa gttggtcaga aactaaaact
     4381 gggtggggcg aaaagtagca gtagtacacg taaatcaacc gccaaatcaa cgactaaaac
     4441 cgcatcggtt acaccgtcat cagcggtacc gaaatcttcc tggccgccag tagggcaacg
     4501 ttgttggtta tggccaacga cagggaaagt tatcatgccg tattcgacag cagatggcgg
     4561 caataaaggg attgatatct cagctccacg gggtacacct atttacgccg cgggtgcagg
     4621 aaaggtggtg tatgtgggca accagctgcg tggctacggt aatctcatca tgattaaaca
     4681 cagtgaagat tacattacgg cttacgccca taatgacacg atgctggtaa ataatgggca
     4741 aagcgtgaag gctgggcaaa aaatcgccac tatggggagc acggatgcgg catctgttcg
     4801 cctgcatttc cagattcgtt accgtgcaac ggcaattgat ccgctacgtt acttgccgcc
     4861 gcagggcagc aagccaaaat gctgatggcg aattaatcag cagtcagcag cgcggcactt
     4921 gctaaggaga gcgtaaggtt tataatgcct tacgcatctc gaagcgggcg tagttcaatg
     4981 gtagaacgag agcttcccaa gctctatacg agggttcgat tcccttcgtc cgctccatga
     5041 cctttcttaa agtactccaa cctactgaat cctaagggat atggctttaa gcctccttgc
     5101 agagttactt actgtaaccc actgtgtaac cctctcaggg catacagaca tatgattcag
     5161 ctatccagta gtcacaaact tccagcagtc tactatctct accagcgtaa cggcgtctac
     5221 tacttccgtc ttcgggtcag acaatcaaac aatgatagaa tgacctcagt atcattacgg
     5281 accaaggata gacgcaccgc tatggctcac tcaaggcaca tcaaggcagc actcaaggca
     5341 atacacgcag acagacccaa cgctacctat gaggagatga gggagcacct taaggacatc
     5401 gctgagtgtg aactgtctat gggccgttca gatctctttg agcctgacat gagggatatc
     5461 taccgggacc aatacggaga acttggtgag agccttaccg atgctctggc tagtgagccg
     5521 ctaagtattg accagcatcg ttacatcaat gaggccctga aggtgctaaa agcctgtatg
     5581 aggcgtatcg aagcgggaga tagtcagcct ctgatagatt acgtcgatcg ttttaatgat
     5641 attgatcggc aagatgacca agcagactct gtgtccctat ctgttagtgc tccagaagta
     5701 aaaccagaag ttacgcctag cattaccata gcgagcctct ttgagcaata cgaagcggag
     5761 aactctcaga actggaagcc tgcaacctta agggaaaaca aagcgtcaca cgctgcgtta
     5821 atcgaaatat tcgaccatct gggtctcaat gcagacgcta acagggctga catgcttaag
     5881 gtccgtgatg tgctccagca gttacctaag aaccgtaagc agcgctttaa ggatgcccca
     5941 cttgctgacc tgttgagccg tgaggataag acagattgtc tggatgtcgt taccatcaac
     6001 aacaagtatc ttattaagat ggctgctgtc tttaggtggg ctgtgcgcaa tgacttgatt
     6061 aaaaagaaca tgactgaggg tctggagctt aaagtccctc agcgtaaagc ctcaggggcc
     6121 cgtaacgcct tctctacgga gcaggtgggg caactactgg tcgcagctaa ggcatactct
     6181 cagaagacct ctggtaagcc ctaccactat tacgttacgg ctttggctgc tatcactggg
     6241 gcgagactca at
//
LOCUS       DAEEUE010000163         6250 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.163, whole genome shotgun sequence.
ACCESSION   DAEEUE010000163 DAEEUE010000000
VERSION     DAEEUE010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6250
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.163"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            63..581
                     /gene="mddA"
                     /locus_tag="KLK84_003823"
     CDS             63..581
                     /gene="mddA"
                     /locus_tag="KLK84_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HBE2010964.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
     gene            578..1027
                     /locus_tag="KLK84_003824"
     CDS             578..1027
                     /locus_tag="KLK84_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBE2010965.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1028..1261)
                     /locus_tag="KLK84_003825"
     CDS             complement(1028..1261)
                     /locus_tag="KLK84_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HBE2010966.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1347..1520)
                     /gene="ortT"
                     /locus_tag="KLK84_003826"
     CDS             complement(1347..1520)
                     /gene="ortT"
                     /locus_tag="KLK84_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HBE2010967.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1715..1810
                     /gene="yncL"
                     /locus_tag="KLK84_003827"
     CDS             1715..1810
                     /gene="yncL"
                     /locus_tag="KLK84_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HBE2010968.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2212..3021)
                     /locus_tag="KLK84_003828"
     CDS             complement(2212..3021)
                     /locus_tag="KLK84_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBE2010969.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3231..4655)
                     /gene="patD"
                     /locus_tag="KLK84_003829"
     CDS             complement(3231..4655)
                     /gene="patD"
                     /locus_tag="KLK84_003829"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HBE2010970.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(4677..5471)
                     /locus_tag="KLK84_003830"
     CDS             complement(4677..5471)
                     /locus_tag="KLK84_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE2010971.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5461..5808)
                     /locus_tag="KLK84_003831"
                     /pseudo
     CDS             complement(5461..5808)
                     /locus_tag="KLK84_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            5836..6081
                     /locus_tag="KLK84_003832"
                     /pseudo
     CDS             5836..6081
                     /locus_tag="KLK84_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558175.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1637 a         1573 c         1419 g         1621 t
ORIGIN      
        1 tgcttcgggc tgttttcgat agtattgata acatcatcat cattatttta ttgaggccag
       61 acatgtccat ccgttttgcc cgcaaagccg actgtgctgc cattgcggaa atttataacc
      121 acgccgtgtt atatacggcg gctatctgga atgaccaaac ggtggatgca gataaccgca
      181 ttgcctggtt tgaagcgcgg accatagcag gttatccggt gctggtaagt gaagaagatg
      241 gcgtagttac gggatatgcc tcgtttggcg actggcgtag tttcgatggt tttcgccata
      301 ccgtggaaca ttcggtttat gtccatcccg atcatcaggg caaaggtctg gggcgtaaat
      361 tgttaagtcg attgattgat gaagcgcggg attgcgggaa gcatgtcatg gtcgccggga
      421 tcgaatcgca aaatcaggct tcgctgcatc tgcaccagtc gctgggattt gtcgtcaccg
      481 cacaaatgcc gcaggtaggc actaaatttg gtcattggct ggatctgaca tttatgcagt
      541 tgcaactcga cgagcgcact gaaccggacg cgattggatg aatcagtcgc tcacccttgc
      601 ttttttgatt gccgccggaa ttggtctggt agtccagaac acgctgatgg cgcgcatcac
      661 ccagacctcc tctaccattc tcatcgccat gttgctgaac tcactggtgg ggattgtgct
      721 ctttgtttct attttatggt ttaaacaagg catggcgggg tttggcgaac tggtgtccag
      781 cgtgcgctgg tggacactta tacctggctt gctgggttcg ttttttgtct tcgccagtat
      841 cagcggatat cagaatgtgg gagcggcaac aactattgcg gtactggtcg ccagccagct
      901 gattggcggt ctggtgctgg atatcttccg cagtcatggc gtaccactac gggcgttgtt
      961 cggcccaata tgtggcgcaa ttttacttgt ggttggcgcc tggttggtgg ccagacgctc
     1021 attttaatca gaggatggtg ccgcctttgg ttaactgttc gtggcgcgct tccatatctt
     1081 ctttatgctt gcgaccgtga tgagcaatag cggtgcgcaa acgctgctgc tgggtgtagc
     1141 gatcttcccg acttaactcc gcgtcatcgc tcagggcgat taataattcg ttcatatggg
     1201 caatgacgct ctcttcaatc gcggcatcca cgcgcgcgat gacttcatcc agatgcgaca
     1261 tactttctcc ttagccactt gtgaaattta tcgattaatc caaatgatat aagtctgcgt
     1321 ggtgccgtca agccagtgag taagatttaa aacagtgaaa acaacagcgc caccgggaaa
     1381 ctcatcggcc atgtggcccc caccagaaag gcgcttaaga aacggatgcg tttcttatcg
     1441 tgagaaagaa accaggtgat aagaaatgcg attgctgcca taaccgcata aaaaacaagc
     1501 atatgttgat agagagacat gtacgggcgt ccagccatat gaataaaacg cgcgcaaata
     1561 tgcgccattt tttgacgtgc atcaagtttt ttcaccagtt tgagaaaaaa tcatcaaaca
     1621 gcaacttgcc cttatccatc ttcagaatga gtattattgc ggattttctt aacccgtact
     1681 atacacatag gggcaatgat aaaaggtggc aaaaatgaat gtttccagta gaactgtagt
     1741 actgataaat gtctttgctg ctgttggttt gtttactctt atctctatga gatttggctg
     1801 gtttatttga tgtagtgatt ttgcacgcag cacgtactgt cagagaaatc aactctctga
     1861 cagtacgcaa gatcttctca tactctccaa gtttgcaaca ttatccttaa tatttacttc
     1921 tttttgctgg catctacatt gaaatgagta gaaaacatcc ccctactgtc gtctatacaa
     1981 acagcttact gattcaaacg gaatcttgtt ttcacatttt tctatcacga atactttata
     2041 cgcgcgggag tctataaatg ctttatactt tcaaggaata acgtaaatat aaaaagatgg
     2101 cgtaaagtat atgcatggca ggatgtttgc aacctccttg atgacgctgc cccattgaaa
     2161 ttgcaaaccc cgccctggtt atcttgccca tttatcatgc aaaacagata attataggcc
     2221 tctgaatacc tcagaaagat tgaatttttt atagtccctc tcttgaatat ttccatttga
     2281 tatcaatcga ttcatctctt caacaaaata atttatatca tcacacttat tattatccgg
     2341 aagatctgag tagttataat tccaccattt tattctcaac agttcatcaa taacatttga
     2401 atcaaaccta aatctgatta tttttgcagg aactcctgca actatggcat atgggggcac
     2461 atctttggta acaacggaat tggcagcaat aacagcacca tcacctattg cgatgccacc
     2521 tttcaataca acatcatttc ctatccagac atcatttcct ataatcggat tttcaggttt
     2581 ggtaatgaat ggtttcaatg tccagctacg ttcaaattca ctcatagcaa acttatcaaa
     2641 ttcaccgtta taagtcatca tgtgagtagt aaaacgattc aaaggatgat ttccacccat
     2701 tatttttaca tttgatgcaa tactacaaaa tcgacctata cgtatattcc ctgaaaaatg
     2761 gcaggtcgga aatgaaaaag cgccgacact ggagaaattt ttcacaggca ttgttgaata
     2821 ttcttcagca ataacattat cttcaattgt tatcttgtca ccttttgaga aaagaaataa
     2881 attcctttct ttatacgact tcttcccctt atgatttaaa aagattttat tatccataca
     2941 aaactcaaag tgttttttcg tccatctgaa ggtaagggtt ttatttttat cttccgtgaa
     3001 tttgtacaat tgaactttca ttgttaatac ttctacttat aaagtattag tttaataacg
     3061 atactctaaa gccttatgct aatcaactgt gacaggatga aaaatcgcca tcaaacattt
     3121 agcctaattt ttacgccagt tattaatgta atcattctaa tacttaaccg ccattaaact
     3181 cagaacctaa aacgatagtg gccgtcctat aacagccact aacatctaac ttaatgttta
     3241 accatgacgt ggcggacgac ggtgtaatcc tccagcccat aaagtgacat atccttgccg
     3301 taaccagaaa gtttctgccc accgtgcggc atttcactta ccagcatgaa atgggtattg
     3361 acccaggtac aaccatattg cagccgtgcg ctgacgcgat gcgccctgcc cacatctttc
     3421 gtccataccg aagatgcaag tccgtactgg ctgtcattcg cccagttcac cacctgttct
     3481 tcgttgtcga agggcgtaac actcactact ggaccaaata cctctttttg cacgatggca
     3541 tcgtcctgta atgcgccagc cagcagcgtc ggcgcatagt aatagccatt acccttgcgc
     3601 ttttcaccgc cagtgatcac tttgatgtgc cctgtcgctt tcgcctcttc tactgccttg
     3661 ctgacgcgtt cgagatgcgc cagcgagctt aaaggtccaa gctccgtaga ctcgtcatcc
     3721 ggcgcaccag attttaacgt tgccaccgca gcacccagtt tttccaccag cgtatcgtaa
     3781 atgccttttt gcgcgtagat ccgacaagcc gcagtacaat cctgtccagc attgtaatag
     3841 ccaaatgtac gtacaccttc gaccactgct tcaatatccg catcatcaaa aacaatcact
     3901 ggcgctttgc caccaagttc catatgagta cgcttaatgg acgacgcggt atggctgatg
     3961 atgtgctcgc cggtggcgat agagcccgtc agcgacacca tccgcacttt gggatgaccg
     4021 gtcagcggat cacccaccgt tttgcctctg ccaaacagta tgttaatcac gcctgccggg
     4081 aagatatctt tcgccagctc tgccaacttc aacgcggtca gcggggtaat ttctgatggt
     4141 ttaagcacta cgcagttccc tgccgccagc gccggagcaa gtttccacgc ggccatcatc
     4201 agcggataat tccacggtgc gatagaagcc acgaccccca acggatcgcg acggatcatc
     4261 gaagtatgac cttcaagata ttcacctgcc gccagaccat tcagacagcg cgccgcaccc
     4321 gcgaaaaagc gaaaaacatc gacaatcgcc gggatttcat cattgaacgc actatgcagc
     4381 ggtttgccac aattacggga ttccagttcg gcaaaaacct gaccattttc ttcgataaca
     4441 tccgccagtt tcagcaggca ttccgcacgc gctttcggtg tggtttgccc ccattcagca
     4501 aatgctgcat ctgccgcacg cacagcagca tcgacctgct ctgcggatac ctcggcaatc
     4561 tccagtaaaa cgtcccccgt tgccggatta tagacaggct gtttttcccc ttcgccgcta
     4621 acccgttctc cgttaatcag taacttatgt tgcatagctt attcctgtat cagtggttat
     4681 tgaccattgt cgccttcgcg ggttagccac caggccccca ggatcggcaa ggttgttacc
     4741 aacataacca gcagtgccac cacgttggtt accggtacat cacgcggtcg cccaagctga
     4801 ttgagcaacc acaacggtaa cgttcgttca tgacctgccg taaaggtcgt aacgatgatt
     4861 tcatcgaacg acaaggcaaa cgccagcatt cctcctgcca gtaacgccga actgagattc
     4921 ggcaacacta cgtagcggaa ggtttgccag ccattggccc caagatccat tgacgcctca
     4981 accagactcc acgaggtgcg gcgaaaacgg gcgatgacat tgttaaacac caccactacg
     5041 caaaaagtcg catgaccgac cacgatggtg aaaaatcccg gctccagatt gatggtttta
     5101 aaggcggtta atagcgccag accagtgaca atgcccggca gcgcaatggg cagcagcaat
     5161 aacagcgaaa tggcgttttt gccgaaaaag tctcgtctcc acagcgcggc agctgccagc
     5221 gtccctaaca ccagcgcaat taatgtcgcc agcgccgcca ctttaagtga cagcgtcacg
     5281 gcatcaagaa tatcactacg ctgtgctgcc acgctaaacc agcgcagcgt caggccctgc
     5341 ggtggaaaac taaacgccgc atcttcagtg ttaaacgcat aggcggcgat tatcaggatg
     5401 ggaaaatgta ggaaaacaac gccgccccag gccgccagtt tgaggaaaaa cggtgcgcgt
     5461 tcagagtgca tcgaacgctc ccagacgttt cacgaacgct aggtacagtg cgatgagaat
     5521 aatcggcacc agggtgaatg ccgccgccat cggcatattg ccaatcgccc cctgctggga
     5581 ataaaccata ttgccgataa aatatcctgg aggaccaacc agctgcggga cgataaaatc
     5641 gcccagtgtg agtgagaagg taaagataga gccagcggca atacccggga ttgccagcgg
     5701 cagcaccaca tagcgaaagg tttgtcgtgg acgtgcgccg agatcagccg acgcctgcaa
     5761 caatgacggc ggcaaacgct caagcgccgc ctgaacgggc aggatcatcg agtaggcagc
     5821 ctgacggctg cggcttgtca tggtctgcaa ttaccgttat aaaaacaggc aatgtcattg
     5881 tctttcaggt ggttatatgg cccgttcagc taaaccccgt aaacgtaaac ccgcctctca
     5941 aagttcctga gtcgtgaccc gttcgaatgc gtgctgtgtg gcggccggat ggtataccgc
     6001 cgggccattg cggggctgaa tgtggagggg ctgaagaaaa acgcgcggga tatcagtctg
     6061 ctgaggtata tgccggcctg acacaggcta agtgtgcccg ggaatcggtg caagtataaa
     6121 aaacagcctg ttgcggtata agaatcgacc atgcagagag agaaaacacc ggagtggagg
     6181 gaaaagcaga aaagctcacg tggcataagg cgtggacagc ggtatcgtct ggtttttcaa
     6241 tttcctatac
//
LOCUS       DAEEUE010000164         6239 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.164, whole genome shotgun sequence.
ACCESSION   DAEEUE010000164 DAEEUE010000000
VERSION     DAEEUE010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6239
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.164"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            393..2051
                     /locus_tag="KLK84_003833"
     CDS             393..2051
                     /locus_tag="KLK84_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HBE2010972.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2048..3004)
                     /locus_tag="KLK84_003834"
     CDS             complement(2048..3004)
                     /locus_tag="KLK84_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HBE2010973.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3155..4270
                     /gene="macA"
                     /locus_tag="KLK84_003835"
     CDS             3155..4270
                     /gene="macA"
                     /locus_tag="KLK84_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HBE2010974.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4267..6213
                     /gene="macB"
                     /locus_tag="KLK84_003836"
     CDS             4267..6213
                     /gene="macB"
                     /locus_tag="KLK84_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HBE2010975.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
BASE COUNT         1469 a         1481 c         1736 g         1553 t
ORIGIN      
        1 aatatggtcg ttttataggg tcgtcgtaaa aaatgacgac ggaaataatg cgcggctatt
       61 ttaaaaacga aggcgagtca ttcaccagat aaataaatcc agtaaatttg atttagggca
      121 acagcgggtt gccccatata gtcatttgtc tgattgacag tgtagtgcaa gcaaaagatt
      181 taatccttta ggcgtaataa aaaataattt atcatgctaa ttatttgatt ttgttgtttt
      241 tgcgggctta tcagcaagag ggagtataac gcgattattc gctcattttt cagacaattg
      301 ccatgcttaa atgtgatgtc atcacgtatt agcaaggcct ttcccgttat actgcgagcg
      361 taaaggataa gtcacatatt tctggagggg atatgattct tgagcgcgtt gaaattgtgg
      421 gttttcgcgg tatcaaccgt ttgtcgttga tgctcgaaca aaacaacgtc ctgattgggg
      481 agaacgcgtg gggtaaatcc agcttgctgg acgccttaac cctgctgcta tcgccagaat
      541 cagatctcta ccattttgag cgcgacgatt tctggttccc gccgggagat atcaacgggc
      601 gagaacatca tctgcatatt attttgacct tccgcgaatc gctgccaggc cgacatcggg
      661 ttcgccgtta tcggccgctg gaagcgtgct ggacgccatg caccgatggc tatcaccgta
      721 ttttttatcg tctggaaggg gagagtgcgg aagacggcag cgtgatgaca ctgcgcagtt
      781 ttctcgataa agacggacat ccgattgatg ttgaggatat taacgatcag gcacgccatc
      841 tggtgcgttt aatgccggtg ctgcgcttgc gtgatgcccg ttttatgcgc cgtattcgta
      901 acggcacggt gccaaatgtc cccaatgtgg aagtcaccgc gcgccagctc gatttcctcg
      961 cccgtgagtt gtcctcacat ccgcaaaatc tctctgatgg acagattcgt cagggacttt
     1021 ccgcaatggt gcagttgctt gagcattatt tctctgagca gggggctgga caggcgcgat
     1081 atcgtttaat gcggcggcgg gccagcaatg agcaacgaag ctggcgctat ctggacatca
     1141 tcaaccggat gattgaccga cctggagggc gctcgtatcg ggttattttg ctcggcctgt
     1201 ttgctacttt attgcaggca aaaggcacat tgcgactgga taaagacgcg cgtccgttat
     1261 tgctaattga agatccagaa acccgcctgc atccgattat gctttccgtt gcctggcatc
     1321 tgttgaatct tttgccttta cagcgtattg ctaccaccaa ctctggcgag ctgctttcgt
     1381 taactccagt ggaacatgtt tgccgcctgg tgcgtgaatc ttctcgtgtt gccgcctggc
     1441 gtctggggcc aagtggcttg agcaccgaag acagccgccg catctctttt cacattcgtt
     1501 ttaatcgtcc gtcatcgctg tttgctcgct gctggttgct ggtggaaggg gaaacggaaa
     1561 cctgggttat caatgaactg gcgcgtcagt gcggacatca ttttgatgcc gaagggatca
     1621 aggtcattga gtttgcccag tccgggctaa agccactggt taaatttgcc cgccgaatgg
     1681 ggattgaatg gcatgtactg gtcgatggcg atgaagcagg gaagaaatat gccgctacgg
     1741 tacgcagcct gttgaataac gatcgggaag ccgaacgaga acatttaacg gcgttaccgg
     1801 cgctggatat ggaacatttt atgtatcgcc agggattttc cgatgtgttc caccgcgtgg
     1861 cgcaaatccc ggaaaatgta ccgatgaatc tacgcaaaat tatctcgaaa gcgatccatc
     1921 gctcttccaa acccgatctt gccattgaag tggcaatgga ggcaggacgt cgtggtgtgg
     1981 actccgtacc aacgctgctg aaaaaaatgt tctcacgcgt gctgtggctg gcgcgcggtc
     2041 gcgcggatta accgcgaaac atcgtggcca tttgtggctg aatagcgtcg agcatctcat
     2101 agcgccgacg gtattcagcc cgttttttac tggcgatttc ggcaatctct tttcgtgcta
     2161 tctgtgctgg aaggcggtaa tggcgttcag catcacatac gccgccaacc gactcccaga
     2221 acgcgttgta atcagcatgg atcttgcctt ctttatcgcg ataacgcagg ctgcggtaaa
     2281 tatgcgtttc attgctgacg gcaataatct gctctacctg caaacgttgg gcaaacagac
     2341 aggccgcttc catcacgagg cgtttgggaa atagcccgtg gcaggctttc gtcgcattct
     2401 ggatttcctg atgtggaatt tcccattttg cgccttgcag tccgccaata aacatcgttc
     2461 ttttcccctg atattcacac agggtaaacg tgatctctgc cagaggaata ccttcgctgt
     2521 tgcggaacag gattgtgctg tcaccttctt tatccattga gatcatcatg gtcagctcaa
     2581 gcgtgaactg ctcaccgttt ttgccttcca gtttagccag ttgcagcccg ggggtattca
     2641 aatataagct gaattcttcc gccgacatac acccgcggag taacgcataa tggtaacgta
     2701 acgcctccag caattgctta cggctaagat tcgcggcaag gtaagggcga tgcagacgca
     2761 caggcagtcg cggctggcgc gttaacaaca cattgagatt aggccagtgg gaaagttcgt
     2821 tcatccactc aacgcttaaa cgcggcataa tcaacgagcg cagcaaaaat ttctggcgaa
     2881 aactacagcg atgccagaat ttacccggcc gacactgtcc acgtgccaga ctaagaaaaa
     2941 gtgacaggct gctgagagat tcagatggcg taaaggtccg ttcagttagc tgcgacatat
     3001 tcatgaaatc aatggttata catgacgtcg atttcaccat tgcgtatctt aaccaaacat
     3061 caatagtgtg attactaacg taaattttag ggttttgttg atatttcgtt gaagttaatg
     3121 acccggattg gcatatggag tattcagaat atttatgaaa aagcggaaaa ccgtgaagaa
     3181 gcgttacgtt attgcgctgg tgatagtcat cgccggactg attacgttat ggagaattct
     3241 taacgcaccc gtgccgactt atcagacact gattgtgcgc cccggtgatt tacagcaaag
     3301 cgtgctggcg accggaaagc tggacgcgct gcgtaaggtt gacgtgggcg cgcaggtcag
     3361 cggtcagttg aaaacgctgt cggtggcgat tggcgataaa gtaaaaaaag accagctttt
     3421 aggggtcatt gatcctgaac aggctgaaaa ccagatcaag gaggtcgaag caacgctgat
     3481 ggagctacgc gcgcagcggc agcaggcgga agcggagctg aaactggcgc gggtgacgta
     3541 ttcccgtcag caacgtctgg cacaaacgca agcggtttca ctgcaggatc tcgataccgc
     3601 cgcgacggag atggctgtga aacaggcgca aattggcacc attgacgcgc aaatcaagcg
     3661 caatcaggcc tctctcgata cggctaaaac caatctcgat tacacccgta tcgttgcccc
     3721 gatggctggg gaagtgacgc aaatcaccac tctgcaaggc cagacggtga ttgccgcaca
     3781 gcaagcaccg aacattctga cgctggcaga tatgagcact atgctggtaa aagcgcaggt
     3841 ttctgaagcg gatgtaatcc acctgaagcc ggggcaaaaa gcctggttta cggtgcttgg
     3901 cgatccactg acgcgctacg aggggcaaat caaggatgtg ctaccgacgc cggaaaaggt
     3961 aaacgacgct attttctatt acgcccgttt tgaagtaccc aaccccaatg gtttgctgcg
     4021 gctggatatg accgcgcaag tgcatattca gctcaccgat gtgaaaaatg tgttgacgat
     4081 ccctctgtcg gcgttaggcg atccggttgg cgataatcgt tataaagtca aattgttgcg
     4141 taatggtgaa acacgcgagc gtgaagtgac gattggcgca cgtaacgata ccgatgttga
     4201 gattgtcaaa gggctggaag cgggcgatga agtggtgatt ggtgaggcca aaccaggagc
     4261 tgcacaatga cgcctttgct cgaattaaag gatattcgtc gcagctatcc tgccggtgat
     4321 gagcaggttg aggtgctgaa gggcatcagc ctcaatattt atgcgggtga gatggtcgcg
     4381 attgttggcg cttcgggttc cggtaaatcg accctgatga atattctcgg ctgcctggat
     4441 aaggccacca gcggcaccta tcgcgtagcc ggtcaggata ttgccacgct ggacgccgat
     4501 gcgctggcgc aactgcgccg cgagcatttc ggctttattt tccagcgtta ccatttgctt
     4561 ccgcatttaa ccgccgagca gaacgttgaa gtacccgccg tctatgccgg tcttgagcgg
     4621 aaacagcgac tgcttcgtgc ccaggagttg ctgcaacggc tggggctgga agaccgtaca
     4681 gagtattatc cggcacagct ttcgggtggt cagcaacagc gcgtcagcat cgcgcgggca
     4741 ttgatgaacg gcggtcaggt aattcttgcc gatgaaccga ccggcgcact ggacagccat
     4801 tctggcgaag aggtgatggc gatcctgcat cagctgcgcg atcgcgggca tacggtgatt
     4861 atcgtcaccc acgatccgca ggtcgctgct caggccgagc gggtgatcga aattcgcgac
     4921 ggcgaaattg tgcgcaatcc tcccgccatt gaaaaagtga atgttgctgg cgggacggag
     4981 cctgttgtca atacagtgtc tggctggcgg cagtttgtca gcggttttaa cgaggcgctg
     5041 acgatggcat ggcgggcgct ggcagcgaat aaaatgcgta ctttactgac catgctgggg
     5101 attattatcg gtattgcgtc ggtggtttcc attgtcgtgg tgggtgacgc cgccaaacaa
     5161 atggtgctgg cggatattcg ttctattggt acgaatacta ttgatgtcta tcccgggaaa
     5221 gattttggcg atgacgatcc gcaatatcag caggcgctga agtacgacga cttaatcgcc
     5281 atccaaaaac aaccgtgggt cgcctcagcc acacctgccg tctcgcaaaa cctgcgcctg
     5341 cgttataaca atgttgatgt tgctgccagt gccaatggcg tgagcggcga ttattttaat
     5401 gtctatggca tgaccttcag tgaaggaaac acctttaatc aggagcagct gaacggtcgt
     5461 gcgcaggtcg tggttctcga cagtaatact cgccgccagc ttttccccca taaagcagat
     5521 gtggttggcg aggtgattct ggtcggcaat atgcccgcca gagtcattgg tgtggcggaa
     5581 gaaaaacagt cgatgtttgg tagcagtaaa gtgctgcgtg tctggctacc ttacagcacg
     5641 atgtccgggc gagttatggg ccagtcgtgg cttaactcca ttactgtcag ggtgaaagaa
     5701 ggatttgaca gcgccgaggc ggaacagcaa ctcacgcgtt tactttcact gcgccacggt
     5761 aagaaggatt tctttacctg gaacatggac ggcgtcttga aaactgttga aaagaccaca
     5821 cgtactttac aactgtttct gacgctggtg gcggtgattt cgctggtggt gggcggtatt
     5881 ggtgtaatga atattatgct ggtgtcagtg accgagcgga cgcgggaaat tggcattcgc
     5941 atggctgtag gtgcgcgagc aagcgatgtt ttgcaacagt tcctgatcga agccgtactg
     6001 gtttgcctgg tcggtggcgc gttgggaata acactgtcac tgttaattgc tttcaccttg
     6061 cagcttttct tacccggctg ggagattggt ttttcaccgt tggcgctgct gctggcgttt
     6121 ctctgctcga cggccaccgg gattttattt ggctggttac ccgcacgaaa tgcggcacga
     6181 ctggatccag tagatgctct ggcacgggag taatttttga gataaaaatg ccagccgat
//
LOCUS       DAEEUE010000165         6212 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.165, whole genome shotgun sequence.
ACCESSION   DAEEUE010000165 DAEEUE010000000
VERSION     DAEEUE010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6212
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.165"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            73..378
                     /locus_tag="KLK84_003837"
     CDS             73..378
                     /locus_tag="KLK84_003837"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE2010976.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /locus_tag="KLK84_003838"
     CDS             complement(454..918)
                     /locus_tag="KLK84_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBE2010977.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /gene="nanK"
                     /locus_tag="KLK84_003839"
     CDS             complement(915..1790)
                     /gene="nanK"
                     /locus_tag="KLK84_003839"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HBE2010978.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1787..2476)
                     /locus_tag="KLK84_003840"
     CDS             complement(1787..2476)
                     /locus_tag="KLK84_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBE2010979.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="KLK84_003841"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="KLK84_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HBE2010980.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="KLK84_003842"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="KLK84_003842"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBE2010981.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="KLK84_003843"
     CDS             complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="KLK84_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HBE2010982.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1575 a         1688 c         1547 g         1402 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcaggatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 attgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgcgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccgtt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatagtgtc cgcagaaggt
     5641 cgagagacac gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
     5701 gccagcgctt cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
     5761 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
     5821 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
     5881 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
     5941 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc
     6001 aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa
     6061 aaattaatgt aacgcagata aacacatccc gattgaatta ttcataagac tattcacaag
     6121 cattatgaat attatgaatg tgttcttaca aaataatcat aatcgcatat tttttaatga
     6181 aaaatcacct cacctacaat taaaaacacg ac
//
LOCUS       DAEEUE010000166         6210 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.166, whole genome shotgun sequence.
ACCESSION   DAEEUE010000166 DAEEUE010000000
VERSION     DAEEUE010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6210
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.166"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..95)
                     /locus_tag="KLK84_003844"
     CDS             complement(<1..95)
                     /locus_tag="KLK84_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE2010983.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYL"
     gene            586..1494
                     /locus_tag="KLK84_003845"
     CDS             586..1494
                     /locus_tag="KLK84_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HBE2010984.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKVEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1540..3531)
                     /gene="tkt"
                     /locus_tag="KLK84_003846"
     CDS             complement(1540..3531)
                     /gene="tkt"
                     /locus_tag="KLK84_003846"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBE2010985.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3809..4567
                     /gene="loiP"
                     /locus_tag="KLK84_003847"
                     /gene_synonym="yggG"
     CDS             3809..4567
                     /gene="loiP"
                     /locus_tag="KLK84_003847"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HBE2010986.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4773..5693)
                     /gene="speB"
                     /locus_tag="KLK84_003848"
     CDS             complement(4773..5693)
                     /gene="speB"
                     /locus_tag="KLK84_003848"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBE2010987.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5829..>6210)
                     /locus_tag="KLK84_003849"
     CDS             complement(5829..>6210)
                     /locus_tag="KLK84_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2010988.1"
                     /translation="VGMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIV
                     NAGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQN
                     ALPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1535 a         1616 c         1599 g         1460 t
ORIGIN      
        1 cagagatagc gctgatgtcc ggcagtgctt ttgccgttac gcaccacgcc ttcagtagcg
       61 gagcaggaag gacatctgat ggaaatggaa gccacgcaag caccttaaaa tcaccatcat
      121 acactaaatc agtaagttgg cagcattacc cttttgctga atgtataact gagattgatg
      181 attctggaaa ataatcacta agccgcataa attatcctta ttttattttt agggtaccgt
      241 ccggcggtaa aaataaaata ctcgtgcggt ttaatgaaag caattgggta cgcgagaaga
      301 aaaaccccac aaaccaaaat aataaaacct taaataacaa caatatgaaa ttgatatttt
      361 taaagtcaac tttgtatttg ttatgttcat cacaaaatca acattttgtt tgttattgta
      421 tttgtgatgc atatctctat ttatttattg aatgttcgaa aacatacttt tctaatgcaa
      481 tgttttttcg ctatacccct ttatagattc tgaattctca cctgtcggct gttttctcct
      541 catctcatgt gacatattcc catactttac tcaaggaaga ttgtcatgaa agcacctgtt
      601 tcattaacct catttcgccc acaaaaatct cttgctatcg ccattggcgt gcttgccgtg
      661 gttgtgctgc catttctctc gtattacacc gttaatgaag gtgaacgcgg cattttgctg
      721 cgctacggaa aaatcgtaaa agttgccgaa ccggggctgg gttttaagat cccttttatg
      781 gaaagcgtgg agaaaatttc cacgcgtaat caggctgtgg tctatcaggg attacaggcc
      841 tacagccgcg accagcaacc ggcacaaatg acagtatcag tcagcttcca cattaaacct
      901 tcagaagcag gcgcggttta taccacctac aacactattg aagcattaaa agatcgtcta
      961 attgtccgtc agttgccaac tcagctggag aacattttcg gccaatatac cgccatcagc
     1021 gccgtacagg atcgtacaaa attggttcag gatctgcaaa atgccatgcg caaagccgtt
     1081 gttgggccag tggtaattga cggggtccag attgaaaata ttgatttttc tgacgcttat
     1141 gaaaagtcga ttgaaaaccg aatgaaagca gaagtcgcca ttgccacgcg taagcaaaat
     1201 ctggaaacag agaaaatcca ggcgcaaata gcggtaactc aggcccaggc agaagcagac
     1261 agtaaactgg cggcagccaa agtagaagcg gaaactattc gcgtaagagg cgcggcagag
     1321 gctgaaacta ttcgtttaaa aagtgcggct gaagcagaag caatccgcct gcgcggtgaa
     1381 gctctgcggg ataatcccgg acttgtagcc ctcaccactg ccgaacgttg ggacggtaaa
     1441 ctgcccgata ccatgatccc cggcagcaca gttccgttta tctctactaa gtaaaaaaag
     1501 ggccgctttc gcggtccttc tcacaaaacg tcttactgat tacagcagcg cttttgcttt
     1561 cgcaacaacg ttatcaacag tgaagccgaa ctcttcaaac agcagctctg ccggagcaga
     1621 ttcaccgaag gtggtcatgc cgacgatagc gccgttcagg ccaacatact tgtaccagta
     1681 gtcagcaata cccgcttcta ccgcaacgcg tgcagtaacc gctttcggca gtacggattc
     1741 acggtaagcc gcatcctgct tgtcaaatgc gtcggtagac ggcatggaca ccacgcgcgc
     1801 tttcacgcct tcggcagtca gtttttcgta ggcagcaacc gccagttcaa cttcagaacc
     1861 ggtggcgatg aagatcagtt ccggctgacc agcacagtct ttcagcacat aaccaccgcg
     1921 cgcgatgttt gccagctgct cttcagtacg ttcctgctgc gccaggttct gacgggagag
     1981 gataagcgca gtcgggccgt cctgacgctc aacgccgtat ttccacgcga ccgcggattc
     2041 aacctggtca cacggacgcc atgtagacat gttcggggtc acgcgcagag aagcgacctg
     2101 ctctaccggc tggtgagtcg ggccatcttc gcccagaccg atggagtcgt gggtgtaaac
     2161 catcacctgg cgctgtttca tcagcgcagc catacgtacg gcgttacgtg cgtattccac
     2221 gaacatcagg aaggtggagg tgtacggcag gaagccaccg tgcagggaga taccgttagc
     2281 aatcgcggtc ataccgaact cgcgaacacc gtagtggatg tagttacctg cagcatcttc
     2341 gttgattgct ttagaaccag accacagggt caggttagac ggtgccaggt cagcagagcc
     2401 gccgaggaat tcaggcaaca gcgggccgaa cgcttcgata gcattctgcg acgctttacg
     2461 gctggcgatt ttcgccggat tagcctgcag tttagcgata aactctttcg ctttggcgtc
     2521 gaagtcagac ggcatttcgc ctttcatacg gcgggtaaat tcagccgctt cctgcggata
     2581 agctttcgcg taagccgcaa acttctcatt ccatgcagat tctttcgcct ggcctgcttc
     2641 tttcgcatcc cactgagcat agatttcaga cgggatttcg aacggtgcat atttccagcc
     2701 cagctgttcg cgggtcaggg caatttcagc gtcgcccagc ggcgcaccgt gggagtcgtg
     2761 agtaccggct ttgttcgggg aaccgaaacc gatgatggtt ttgcacatca gcagggacgg
     2821 tttgtcagtc actgcgcgcg cttcttcaac tgcgcgtttg atggatgccg cgtcatgacc
     2881 gtcgatgtcg cgaataacgt gccagccgta agcttcgaaa cgcattgctg tgtcgtcggt
     2941 gaaccagcct tcaacgtgac catcgataga aataccgttg tcatcgtaga acgcaatcag
     3001 tttacccagc ttcagcgtac ccgccagaga gcaaacttcg tgggagatgc cttccatcat
     3061 gcagccgtcg cccatgaagg cgtaggtgta gtggtcgaca atgtcgtggc ccggacggtt
     3121 aaactgcgcc gccagcgttt tttctgcaat cgccataccg actgcgttgg caataccctg
     3181 acccagcgga ccggtggtgg tttccacacc agcggtataa cctacttccg ggtggcccgg
     3241 agttttagag tgcagctgac ggaagttttt cagttcttcc atcggcagat cgtaaccggt
     3301 gaggtgcagc aggctgtaga tcagcatgga gccgtggccg ttggacagca cgaagcggtc
     3361 acggtcagcc caggacggat tctgcgggtt gtgtttcagg aaatcacgcc acaggacttc
     3421 ggcaatgtca gccataccca taggggcacc cgggtgaccg gatttggctt tctgtactgc
     3481 gtccatgctc agcgcacgaa tagcattggc aagctcttta cgtgaggaca ttttgactcc
     3541 agatcggatg atgaagggca cgcccttaac gacttgacga cagcgcgttt tgggctacgc
     3601 cggaaaattt gccaacaatt taccgcaagc cgcgcgtcat gtacatggaa catccttttg
     3661 ccgcttcaga aatctctgga tcatgctcgc atgttgcgca atctgctcgc ccgtccgctg
     3721 cgcttttcct tatactgaga ctgagcgtcg attcacctgc aaacggcgca tttttagaat
     3781 aatcctgacc ttgtgcggaa gagaaaacat gaaaattcgc gccttattgg tagcaatgag
     3841 cgtggcaacg gtactgaccg gttgccagaa tatggactcc aacggactgc tctcatcagg
     3901 agcggaagct tttcaggctt acagtttgag tgatgcgcag gtgaaaaccc tgagcgatca
     3961 ggcatgtcag gagatggaca gcaaggcgac gattgcgcca gccaatagcg aatacgctaa
     4021 acgtctgaca actattgcca atgcgctagg caacaatatc aacggtcagc cggtgaatta
     4081 caaagtgtat atggcgaagg atgtgaacgc ctttgcaatg gctaacggct gtatccgcgt
     4141 ctatagcggg ctgatggata tgatgacaga taacgaagtc gaagcggtga tcggtcacga
     4201 gatggggcac gtggcgttag gccatgtgaa gaaagggatg caggtggcgc ttggtacaaa
     4261 tgccgtgcga gtagctgcgg cctctgcggg cgggattgtc ggcagtttat ctcaatcaca
     4321 acttggtgat ctgggcgaga aattagtcaa ttcgcaattc tcccagcgtc aggaagcaga
     4381 agccgatgat tattcttacg atcttctgca ccaacgcggc atcagcccgg caggtcttgc
     4441 caccagcttt gaaaaactgg cgaagctgga agaaggccgt caaagctcaa tgtttgacga
     4501 ccatcctgca tccgccgaac gtgcccagca tattcgcgat cgcatcagcg cggatgggat
     4561 taagtaaagc ctagtggtgt cgaacgtagg tcagataagg cgttcacgcc gcatctgaca
     4621 ttaatggcac atttgttacc ttgtgcgcat tgccggatgc gatgctggcg catcttatcc
     4681 ggcctacggg tgccgaacgt aggtcggata aggcgttcac gccgcatccg acattaatgg
     4741 cacgttttac ccgtgcgcat cgcatctggt gcttactcgc cctttttcgc cgcctgaata
     4801 tacagcatct ccagcgccag cgtcgccgca gccagagcgg tgatttccga ctgatcgtat
     4861 gccggagcca cttccactac gtccatccca acgatgttga gatctttcag gccgcgtacc
     4921 agtttaatgg cgcgatcgga ggtcaggccg ccaatcactg gcgtaccggt tcccggcgcg
     4981 aaagcaggat ccaggcagtc gatatcaaaa gtcaggtaaa ccggcatatc acccacaatc
     5041 tgtttcacct gggcgataac gtcatccacg ctgcgatcgt tcacctggca ggcgtccagc
     5101 acggtaaagc cgttgtcttt atcaaactcg gtacgaatac caatctgcac ggaatgattc
     5161 gggtcgatca gaccttcttt cggcgcggta tagaacatag tgccgtggtc aaattcacaa
     5221 ccgttcgcat aggtatcggt gtgggcgtca aagtgtacca gcgccatttt gccgaaatgc
     5281 ttcgcatgag cacgcagcag cggcagcgta acaaagtggt caccaccgaa ggagagcata
     5341 cgcttaccgg cagccagcag cttctcggcg tgcgcctgca gcttttcgct catctcacgg
     5401 gcatcgccaa aggcatatac cagatcgccg cagtccacga cgttcagacg ctcacgcatg
     5461 tcgaaattcc acgggaagcg gttgtgttcc caggccagat tcgtcgaaac ctgacggatc
     5521 gctgccggac cgtggcgacc acccgcacga ccagaagtgg ccatatcgaa cggcacgcca
     5581 gtaatcaccc agtctgcatc gctgtcatac ggctggaagt tcatcggcag gcgtaaaaaa
     5641 ccaaaggcat tggaaaccag tgagttatcg tattgatgac ctaaggtgct catggatgga
     5701 ctcctattac aaagtcgata taaaaaaacc cttccgcgtc gttaggcccg acgaggaagg
     5761 gttggatttg cgaaacacaa gtttgtgcta attatcgcct taaatgggcg aggcatcaag
     5821 cgaggttgct atttccgaca cggtggcggc gcatagccat tgttatctgc cggaaaggct
     5881 tgtccagagc acggcggcag cgcgttttgc gccgggcctt gtaattcaaa ggcggcaaag
     5941 ccgattaccc cggtattacg cacatcgcct tgcgccgaat tggctgcgta ggaatcagca
     6001 acgttggcga actgaaaagc cgcttcagta tgtttatctt tgcggaatcc tttaatcact
     6061 aacgaatctc cggcattcac aatatagccg ttgttattca gcgaaccttg cttgccgttc
     6121 agcacatcca ggccatcagc cgtagcaacg atctcgtagt tggtgttacg actgtaatta
     6181 cggacatata attgataatt cattccgact
//
LOCUS       DAEEUE010000167         6059 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.167, whole genome shotgun sequence.
ACCESSION   DAEEUE010000167 DAEEUE010000000
VERSION     DAEEUE010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6059
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.167"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            382..1410
                     /gene="torT"
                     /locus_tag="KLK84_003850"
     CDS             382..1410
                     /gene="torT"
                     /locus_tag="KLK84_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HBE2010989.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(1383..2075)
                     /gene="torR"
                     /locus_tag="KLK84_003851"
     CDS             complement(1383..2075)
                     /gene="torR"
                     /locus_tag="KLK84_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HBE2010990.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            2162..3334
                     /gene="torC"
                     /locus_tag="KLK84_003852"
     CDS             2162..3334
                     /gene="torC"
                     /locus_tag="KLK84_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HBE2010991.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            3334..5874
                     /gene="torA"
                     /locus_tag="KLK84_003853"
     CDS             3334..5874
                     /gene="torA"
                     /locus_tag="KLK84_003853"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HBE2010992.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRAMVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEVEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIPIA
                     RFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVIAI
                     DNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDIFR
                     ELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFDHP
                     QMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGGPG
                     SQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDVVR
                     VFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIGTS
                     QLAQAASAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            5871..>6059
                     /gene="torD"
                     /locus_tag="KLK84_003854"
     CDS             5871..>6059
                     /gene="torD"
                     /locus_tag="KLK84_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HBE2010993.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         1471 a         1516 c         1680 g         1392 t
ORIGIN      
        1 tccatttcgg tcccagttag gacgctttct catcaagcca gtccgcccac cactgcatca
       61 tctccctacg cttatcgaga tactgagcat ggttgtaaat tcaattaacc cagtgcgttg
      121 acaaaaaatt agcgcaagaa gacaaaaatc accttgcgct aatgctctgt tacaggtcac
      181 taataccatc taagtggttg attcatagtg actggatatg ttgtgttttg tcgcgttttt
      241 cagtctatta tttaatcatt aagtaatcta atgctatgat ttttaaatta tttatgtgat
      301 tttgtcatcc tttaggtgaa aaaggttgag tcgcaaagtg gaatgcatct agcataaagc
      361 cttattattg atgaggctat catgcgcgta ctgctatttt tacttctttc ccttttcatg
      421 ttgtcggcat tttcggctga taacctgttg cgctggcatg atgcgcagca tttcacggtg
      481 caagcctcta tgccgcttaa agccaaacgc gtatggaaac tgtgcgcgct ttatcccagc
      541 ctgaaagatt catactggtt atcgttgaac tatggtatgc aggaggctgc tcgccgctac
      601 ggtgtggatt taaaagtgct ggaggcaggc ggctacagcc agttggctac ccagcaagca
      661 caaatcgacc agtgtaaaca gtggggcgca gaggccattt tgctcggtag tagcacgacg
      721 tcatttcccg acctgcaaaa gcaggtagca aatctgccgg tgatcgaact ggtaaatgct
      781 cttgatgatc cccaggtgaa aagccgcgtt ggtgtaccct ggtttcagat gggctatcaa
      841 cctgggcgat atttggtgca atgggcgcac ggtaaaccac tgaacgtgct gctgatgccc
      901 ggacccgata acgccggggg cagtaaggag atggtcgagg gttttcgcgc agccattgcc
      961 ggaagcccgg tgcgtattgt tgatattgcg cttggtgata acgatattga aatccagcgc
     1021 aacctgttgc aggagatgct ggaacgccac ccagaaatcg acgtcgttgc cggaacggcc
     1081 attgcggcag aggcggcaat gggtgaaggg cgtaacctga aaacgccgct taccgtggtg
     1141 tcgttttatc tttcacatca ggtgtatcgc gggctgaagc ggggaagagt gattatggct
     1201 gccagcgatc aaatggtctg gcagggggaa ctggcagttg agcaggccat caggcaatta
     1261 caggggcaat cggtgtctga taatgtcagc ccaccgattt tagttctgac gccgaaaaat
     1321 gccgaccgag aacatatccg ccgctcgctg tcaccggggg gatttcgtcc ggtctatttt
     1381 tatcagcaca catcagcggc taagaaataa ccttcaccat gttgcgtcac cagtaaatcc
     1441 gcgctgagtt tatgacgtaa acgacgaatt aacacatcga cggtgcgcag gtcagggttt
     1501 tccacccgac gcgcagaaag catacgcagc agacgctcac ggctgagaat ttcgcccgga
     1561 ttagtcacaa atgccaccaa catttcatac tctgcgcggg tcagtttaat cggctcgcca
     1621 tcccgctcca gcgtatggcg cgacacattc aggcaataac ccgcaaagcg atagcagtag
     1681 tcctgagtgt gcggttgagc ttgtcgcgcg aggtcgattc gccagagcag atttttcacc
     1741 cgtactacca gttcgcgcag ttccagcggt ttggtgacgt aatcgtctgc gcccatttcc
     1801 agcccaacaa tacggtcaat ccgatcgctg cgtccggtaa ccagaataat ccccaccgtt
     1861 gagcgttctc gcagggcgcg ggttaacatc aggccatttt catcgggtaa gttgatatcc
     1921 agcagaatta aatctaccgg ctgattctgc ataatttccc gtagcccggc accgctcgcc
     1981 gtaacggaaa cggtataccc ctcctgagtg aagtaggatt gtaatcgcgc ctgggtaacc
     2041 ggcttatctt caacaataac aatgtgatgt ggcatcagag ggttttactc attctgttca
     2101 tatctgttca tatttgctca ttaagatcgc ttcactaaac cataattcta caggggttat
     2161 tatgcggaaa ctctggaacg cgctacgccg acccagtgct cgttggtcgg tactggcgct
     2221 ggtcgcaatt gggattgtga ttggcattgc gctgattgta ttgccacacg ttgggatcaa
     2281 agtcaccagc acaaccgaat tttgtgtcag ttgccacagt atgcaaccgg tgtatgaaga
     2341 atataaacag tcggtgcatt tccagaacgc ctccggcgtg cgagctgaat gccatgactg
     2401 tcatatcccg ccggatattc caggcatggt gaagcgcaaa ctggaagcga gcaacgacat
     2461 ttaccagacc tttattgccc actccattga tacacctgaa aaattcgaag ccaaacgcgc
     2521 ggaacttgcc gagcgtgaat gggcgcgaat gaaagaaaac aactcggcaa cctgtcgctc
     2581 ctgccataac tacgatgcga tggatcatgc caggcagcat cctgaagcgg cacgtcagat
     2641 gaaggtggca gcgaaagata atcaatcctg catcgactgt cataaaggta ttgcccacca
     2701 gttaccggat atgagtagcg gcttccgtaa gcagttcgat gagctgcgcg ccagtgctaa
     2761 tgacagtggt gacacgctgt actctattga tattaagcca atttatgcgg cgaaaggcga
     2821 taaagaagcc tctggttctc tgctgcctgc ttcggaagtg aaagtcctta aacgtgacgg
     2881 cgactggctg caaattgaaa ttaccggctg gacggaaagc gccggacgtc agcgtgtact
     2941 cacccaattc ccaggtaaac gcatctttgt tgcctcgatt cgtggtgatg tgcagcagca
     3001 ggtgaaaacg ctggagaaaa ccaccgttgc cgacaccaat accgagtgga gcaagttgca
     3061 ggccaccgcg tggatgaaga aaggcgacat ggtgaacgat atcaaaccga tctgggccta
     3121 tgcggattcg ttgtacaacg gcacctgtaa ccagtgccac ggcgcaccgg aaatcgccca
     3181 ctttgacgct aacggttgga tcggcacgct caacggcatg attggcttta ccagtctcga
     3241 taaacgtgaa gaacgcactt tgttgaaata tctgcaaatg aatgcgtctg acaccgcagg
     3301 taaggctcac ggcgataaga aggaagaaaa ataatgaaca ataacgatct ctttcaggca
     3361 tcacgtcggc gttttctggc acaacttggc ggcttaaccg tcgccgggat gctggggccg
     3421 tcattgttaa cgccgcgccg tgcaactgcg gcgcaagcgg cgactgaggc tgtcatctcg
     3481 aaagagggca ttcttaccgg gtcgcactgg ggggctatcc gcgcgatggt gaaggatggt
     3541 cgctttgtgg cggcaaaacc gttcgaactg gataaatatc cgtcgaaaat gatcgccgga
     3601 ttgccggatc acgtacacaa cgcggcacgt attcgttatc cgatggtacg cgtggactgg
     3661 ctgcgtaagc gccatctgag cgacacctcc cagcgcggtg ataaccgttt tgtgcgcgtg
     3721 agctgggatg aagccctcga catgttctat gaagaactgg aacgcgtaca gaaaactcac
     3781 gggccgagtg ccttgctgac cgccagtggt tggcaatcga cggggatgtt ccataacgct
     3841 tcggggatgc tggcgaaagc tattgccttg catggtaata gcgttggtac gggcggagat
     3901 tactctaccg gtgctgcgca ggtgatcctg ccgcgcgtag tcggttcaat ggaagtgtat
     3961 gaacagcaaa cctcctggcc gctggtattg cagaacagca aaaccattgt gctgtggggc
     4021 tccgatttgc tgaaaaacca gcaagcgaac tggtggtgcc cggatcacga tgtttatgaa
     4081 tattacgagc agctaaaagc gaaagtcgcc gccggtgaag ttgaggtcat cagcatcgat
     4141 ccggttgtca catccaccca tgagtatctg gggcgcgagc atgtgaagca cattgcggtt
     4201 aacccgcaaa ctgacgtgcc gctgcaactg gcgctggcac atacgctgta cagtgaaaac
     4261 ctgtacgaca aaaacttcct cgctaactac tgtgtgggtt ttgagcagtt cctgccgtat
     4321 ctgctgggtg agaaagacgg tcagccgaaa gatgccgcat gggctgaaaa actgaccggc
     4381 attgatgccg aaaccattcg tgggctggcg cggcagatgg cggcgaacag aacgcaaatt
     4441 attgctggct ggtgcgtgca gcgtatgcag cacggtgaac agtgggcgtg gatgattgtg
     4501 gttctggcgg cgatgctggg gcaaattggc ctgccaggtg gtggttttgg ctggcactac
     4561 aacggcgcag gcacgccggg gcgtaaaggc gttattctga gtggtttctc cggctctacg
     4621 tcgattccgc ctgttcacga caacagtgac tacaaaggct acagcagcac tattccgatt
     4681 gcccgtttta tcgatgcgat cctcgaaccg gggaaagtga tcaactggaa cggtaaatcg
     4741 gtaaaactgc cgccgctgaa aatgtgtatt tttgccggaa ctaacccatt ccatcgccat
     4801 cagcagatca accgcattat tgaaggctgg cgcaagctgg aaacggttat cgccatagat
     4861 aaccagtgga cctcaacctg ccgctttgcc gatatcgtgc tgcctgcgac cacgcagttt
     4921 gagcgtaacg atctcgacca gtacggcaac cactccaacc gtggcattat cgccatgaaa
     4981 caggtggtgc cgccgcagtt cgaggcgcgc aacgactttg atattttccg cgagctgtgc
     5041 cgtcgcttta atcgtgaaga agcctttacc gaagggctgg acgaaatggg ctggctgaaa
     5101 cgcatctggc aggaaggtgt acaacaaggc aaaggacgcg gcgttcatct gccagcgttt
     5161 gatgacttct ggaataacaa agagtacgtc gagtttgacc atccgcagat gtttgttcgc
     5221 caccaggcat tccgcgaaga tccggatctc gaaccgctgg gcacgccgag tggcctgatt
     5281 gagatctact cgaaaaccat cgccgatatg aactacgacg attgtcaggg gcatccgatg
     5341 tggtttgaga aaatcgaacg ctcccacggt gggcccggct cgcagaagta tccgttgcat
     5401 ctgcaatctg tgcatccgga tttccgactt cactcgcagc tatgtgagtc ggaaacgctg
     5461 cgtcagcaat atacggtagc gggtaaagag ccagtgttca ttaacccgca ggatgccagc
     5521 gcgcgcggta ttcgtaacgg tgatgtggta cgcgtcttta acgctcgcgg tcaggtgttg
     5581 gcaggagcag tagtttctga ccgctatgca cccggcgtgg cgcgaattca cgaaggggca
     5641 tggtacgatc cagataaagg cggcgagcct ggtgcgctgt gcaaatacgg taaccccaac
     5701 gtgctgacca tcgacatcgg gacttcgcag ctggcgcagg cggccagtgc gcacactacg
     5761 ctggtggaaa ttgagaagta caatggaaca gtggagcagg tgacggcgtt taacggcccc
     5821 gtggagatgg tggcgcagtg cgaatatgtt cccgcgtcgc aggtgaaatc atgaccacgc
     5881 tgacagcaca acagattgcc tgtgtttacg cctggctggc gcagttgttc tcccgtgaac
     5941 tggacgatga acaactgacg caaatcgcca gtgcgcagat ggctgaatgg ttttcgttgc
     6001 tgaaaagcga accgccgctc actgcggcgg tgaatgatct ggaaaacagt attgccacg
//
LOCUS       DAEEUE010000168         6034 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.168, whole genome shotgun sequence.
ACCESSION   DAEEUE010000168 DAEEUE010000000
VERSION     DAEEUE010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6034
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.168"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            92..868
                     /gene="exuR"
                     /locus_tag="KLK84_003855"
     CDS             92..868
                     /gene="exuR"
                     /locus_tag="KLK84_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HBE2010994.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1213..1875
                     /locus_tag="KLK84_003856"
     CDS             1213..1875
                     /locus_tag="KLK84_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBE2010995.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1879..2262
                     /gene="mzrA"
                     /locus_tag="KLK84_003857"
     CDS             1879..2262
                     /gene="mzrA"
                     /locus_tag="KLK84_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HBE2010996.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2409..2763
                     /locus_tag="KLK84_003858"
                     /pseudo
     CDS             2409..2763
                     /locus_tag="KLK84_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2801..3106
                     /locus_tag="KLK84_003859"
     CDS             2801..3106
                     /locus_tag="KLK84_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HBE2010997.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3109..3513
                     /locus_tag="KLK84_003860"
     CDS             3109..3513
                     /locus_tag="KLK84_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBE2010998.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3503..3802
                     /locus_tag="KLK84_003861"
     CDS             3503..3802
                     /locus_tag="KLK84_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HBE2010999.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3988..4284
                     /locus_tag="KLK84_003862"
     CDS             3988..4284
                     /locus_tag="KLK84_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBE2011000.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4354..5340
                     /gene="yqjG"
                     /locus_tag="KLK84_003863"
     CDS             4354..5340
                     /gene="yqjG"
                     /locus_tag="KLK84_003863"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HBE2011001.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5633..6001
                     /locus_tag="KLK84_003864"
     CDS             5633..6001
                     /locus_tag="KLK84_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBE2011002.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
BASE COUNT         1527 a         1471 c         1519 g         1517 t
ORIGIN      
        1 attcaatcat caaatttata tatgaccaat ggcataccca tgaataaagt ggtatgataa
       61 cttcgtccgc attccactcc gggagacgca tatggaaatc actgaaccac gccgtttgta
      121 tcaacaactt gctgctgacc tgaaagagcg cattgaacag ggcgtctatc tggtgggtga
      181 taaactgcct gccgaacgct ttattgccga tgaaaagaac gtcagccgaa cggtagttcg
      241 tgaagccatc atcatgctgg aagttgaagg ctatgtggag gtgcgtaaag gttcaggtat
      301 tcatgtggtt tccaaccagc cgcgtcatca gcaggcggct gacaataata tggaattcgc
      361 caattacggt ccgtttgagt tgcttcaggc tcgccagctc atcgaaagta atattgccga
      421 gttcgcggca actcaggtaa cgaaacaaga catcatgaaa ctgatggcca ttcaggaaca
      481 ggcgcgcggc gaacaatgct tccgtgattc cgagtgggat ttacagttcc acattcaggt
      541 agccctggcg acgcagaact ccgccctggc ggccatcgtt gaaaaaatgt ggacccagcg
      601 tagccataac ccgtactgga aaaaactaca cgaacacatt gattcccgta ccgttgataa
      661 ctggtgtgat gaccacgatc aaattctcaa ggcgttaatt cgcaaagatc ctcatgctgc
      721 taagctggca atgtggcagc atctggaaaa caccaaaatc atgttattta acgaaaccag
      781 cgacgatttc gagttcaatg ccgaccgcta tctgttcgcc gaaaacccgg tagtgcatct
      841 cgatacggcc accagcggca gcaaatgaaa ttcctgtccg acaggcgctg caatgcgcct
      901 gtttgtctcg tcggtaagcg aaaagtataa agtgtcagcc tgtgtaaatc ctctcgccac
      961 cctcccctgc aatcagcaaa atcagactcc acggacatgt aattttgata acgaacaacg
     1021 ttgacctttg ttacaattag attcaatttg aatttatgct tttgaatgct ttcttatctc
     1081 acgttttaac agggaatagt tcaggctgtg ttgatgtatc aaacccgcag aacataccaa
     1141 aacagcaata acattgcggt agtgcatctt ttaaaaccag cgtggcgtta accgattcac
     1201 caggaataat gaatggaact tttgacccaa ttgctgcaag ccctgtgggc gcaggatttt
     1261 gaaaccctgg ccaatccatc gatgattggc atgttgtatt ttgtcttgtt tgtaattttg
     1321 ttccttgaaa atggcttgct tccggcggcc tttttaccgg gcgacagttt actggtattg
     1381 gtcggcgtgt tgattgcgaa aggcgcgatg ggttacccgc aaacgattct gctgctgacc
     1441 gttgccgcca gcctcggttg ctgggtcagc tatattcagg ggcgatggct gggcaatacc
     1501 cgcaccgtac aaaactggct atctcattta cccgcgcatt atcatcaacg cgcacaccat
     1561 ctttttcata aacacggttt atcggcgctg ttaattggtc gctttattgc atttgtcaga
     1621 acactgctgc cgacgattgc cgggttatca gggctgaata acgcgcgctt tcagttcttc
     1681 aactggatga gcggtctgct gtgggtattg atcctgacaa ctctgggtta catgctcggc
     1741 aaaacgccgg tatttttaaa gtacgaggac cagctgatgt catgcctgat gctgctcccg
     1801 gtggtgctgc tggtgtttgg cctggcaggt tctctggtcg tgttatggaa aaagaaatat
     1861 ggaaatcggg ggtaagggat gcaaatacct cgcatgtcgc ttcgccagct agcctggtcc
     1921 ggcactgttt tacttctggt cggcacgctg ttgctggcct ggtccgcggt tcgccagcaa
     1981 gagtctacgc tggcgattcg tgccgttcat caaggcacaa cgatgccaga cggtttttcg
     2041 atctggcatc accttgacgc tcatggcatt cctttcaaaa gtatcacccc caaaaacgac
     2101 actctgttaa ttacatttga ctccagcgac cagagcgccg ccgcaaaagc ggtcctcgac
     2161 agaacattgc cccatggcta catcattgcg cagcaggaca ataacagtca ggctatgcag
     2221 tggctgaccc ggttacggga taactctcat cgcttcggat aacttcctgt tatccgaaac
     2281 atatcactca ctttggtgat ttcaccgtaa ctgtctatga ttaatgaggc ggtggttaac
     2341 actaccgctt cgttgtctgg acaatcgttc ctttgtaata ggtccgaatc acaatggaag
     2401 gttcaagaat gaaataccgc atcgctttag ctgtttctct ctttgctctt agtgccggta
     2461 gttatgccac taccctgtgt caggaaaagg agcaaaatat ccttaaggag atcagctatg
     2521 ccgaaaaaca ccaaaaccag aatcgtattg acggtctgaa taaagccctg agtgaagtcc
     2581 gggccaactg ttcagatagc cagctgcgtg ccgatcatca caagaaaatc gcaaagcaga
     2641 aagatgaggt ggcggaacgc cagcaagatt tagccgaggc gaagcaaaaa ggcgatgccg
     2701 atagaacgga aactggcaga agcgcaggaa gagctgaaaa agctggaagc gcgcgactac
     2761 taactcacaa tagtcactac ttactcacct ggagaaaact atgtcgaaag aacacactac
     2821 ggaacatctg cgtgctgagt tgaaatccct ttccgatacg ctggaagagg tgcttagctc
     2881 atctggcgag aagtcgaaag aagagttgag taagattcgt agcaaagcgg agcaggcact
     2941 gaaacagagc cgttatcgcc tgggtgaaac cggtgatgcc atcgccaaac aaacccgtgt
     3001 cgcggcggcg cgtgctgatg agtatgtgcg tgaaaatccg tggaccggcg tgggaattgg
     3061 cgctgcaatc ggtgtagtgc tcggcgttct gctgtcgcgt cgttaattat ggcggacact
     3121 catcacgcgc aagggcccgg taaaagcgtt ctgggcatcg ggcagcgaat tgtttccatc
     3181 atggttgaaa tggtggaaac acgtctgcgg ctggcggtgg tggagctgga agaggaaaaa
     3241 gctaatctct ttcaactttt actgatgctg ggcctgacga tgcttttcgc tgcatttggt
     3301 cttatgagcc tgatggtgct aattatttgg gcggttgatc cgcaatatcg cctgaatgcg
     3361 atgattgcca ccaccgtggt gttgctgcta ctggcactga ttggcggtat ctggacgtta
     3421 cgtaaatcgc gtaaatccac cttgctgcgc catacacgcc atgagctggc aaacgatcgg
     3481 caactgctcg aggaggagtc ccgtgagcag taaagtcgaa cgtgaacgac gtaaggcgca
     3541 actgcttagc cagatccagc aacaacggct ggatctttcc gccagtcgtc gtgaatggct
     3601 ggaggcaaca ggcgcttacg atcgtcgctg gaatatgctg ctaagtctgc gctcctgggc
     3661 gctggttggc agtagcgtga tggcgatctg gacgattcgc catcctaata tgctggtccg
     3721 ctgggccaga cgcggttttg gcgtatggag cgcctggcgt ctggttaaaa cgaccctcaa
     3781 gcagcaacag cttcgcggtt aacttccctc tggccggagc aattccggcc ttatccctca
     3841 aattttttga atatttttga cagttttcct tgctaacaat catcattcac cacgtttatg
     3901 attctctcca tcgacagcaa cgacgctaat agcgcgccct tgcacaaaaa aacaatcagc
     3961 agcctgagtg gcgcagtgga gagtatgatg aaaaaattag aagatgttgg tgtactggta
     4021 gcgcacattt taatgccgat tctgtttatt accgcaggct ggggaaaaat caccgcttac
     4081 tctggtaccc aacaatatat ggaagcaatg ggtgtcccgg gttttatgct gctgacggca
     4141 tttttatttc acagcaactt tgctgaaggc gtcaactcgc tgatgttcat gaaaaacctg
     4201 acaatttctg gcggattcct gctgctggca attaccggtc cgggcgcgta tagcatcgac
     4261 cgcctgctga ataaaaagtg gtaagcacgc gctatactta acgataaaaa acgaggagga
     4321 agctcctcgt ttttgctatt ggaggagaga aaaatgggtc aactgattga cggcgtctgg
     4381 catgacacct ggtacgatac caaatctacc ggcggtaaat ttcaacgttc agcttccgca
     4441 tttcgtaact ggctcactgc cgatggcgca cctggcccca ctggcacagg cggttttatc
     4501 gcagagaaag atcgttatca tctctatgtt tcactcgcct gcccgtgggc gcaccgcacg
     4561 ctgatcatgc gcaaactcaa aggactggaa ccgtttattt ccgtttccgt agtgaacccg
     4621 ctgatgctgg aaaacggctg gacctttgat gacagttttc cgggagcaac cggagatacg
     4681 ctctatcaac atgaatttct gtatcagctt tatctccacg ccgatccaca ctacagcgga
     4741 cgagttactg ttcccgtgct gtgggacaaa aagaaccaca ccatcgtcag caacgaatca
     4801 gcagaaatca tccgcatgtt caataccgcg tttgatgcgc tgggcgcaaa agcgggtgat
     4861 tactacccac cagccctgca aacgaaaatt gacgaactta acggctggat ttatgacacc
     4921 gttaacaacg gcgtgtataa agctggtttt gccaccagcc agcaagccta cgacgaggcg
     4981 gtggcgaaag tgtttgaatc gctggcgcga ctggagcaga ttttaggcca gcatcgttac
     5041 ctgaccggca accagctaac cgaagccgat attcgcctgt ggactacgct ggtgcgtttt
     5101 gatccagtgt atgtgaccca cttcaagtgt gataagcacc gcatcagcga ttacctgaat
     5161 ctgtatggct tcctgcgcga tatctaccag atgccgggga tcgccgaaac tgtcaatttc
     5221 gatcacatcc gtaatcatta cttccgcagc cataagacca tcaaccctac ggggattatt
     5281 tcaattggtc cgtggcagga tctcgatgaa ccgcatggac gagatgttcg cttcggttaa
     5341 aaaaaggcac cttacgaggt gcctctgata attagctaag tgcatgttgt tcatgtcgga
     5401 tgcggcgtga acgccttatc aaacctacaa agcgtttata ttcaatatgt tacaatgacc
     5461 gcgtaggcca gataagcgta gcgcatctgg cgattttgcg cttgccatca gtcttagcgc
     5521 gccttcacgg cactttatta attccttgaa cgaatattta ctgccatttc attcacgtct
     5581 attcttaatt tgctgcttta aaaacaagtg attgagcaaa attgaggcaa aaatggactg
     5641 gtatctgaaa gtactaaaaa attatgtcgg tttccggggt cgtgcgcggc gcaaagagta
     5701 ctggatgttt attctggtca acatcatctt tacgttcgtg ctggggctgc tggataaaat
     5761 gttaggctgg caacgcgctg gtggcgaagg catcctgacg ataatctacg gtattctggt
     5821 gtttttaccg tggtgggcgg ttcagttccg ccgcctgcac gacaccgacc gctcggcgtg
     5881 gtgggcactg ctattcttaa tcccgtttat cggctggctt attatcatcg tctttaactg
     5941 ccaggcgggt acgccaggcg aaaaccgctt tggaccagat ccaaaactgg aacaagaata
     6001 atgattaagc accttgtaaa aggtgcttca aatt
//
LOCUS       DAEEUE010000169         5981 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.169, whole genome shotgun sequence.
ACCESSION   DAEEUE010000169 DAEEUE010000000
VERSION     DAEEUE010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.169"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..262)
                     /locus_tag="KLK84_003865"
     CDS             complement(<1..262)
                     /locus_tag="KLK84_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBE2011003.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFV"
     gene            483..911
                     /gene="uspG"
                     /locus_tag="KLK84_003866"
     CDS             483..911
                     /gene="uspG"
                     /locus_tag="KLK84_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HBE2011004.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(1032..2597)
                     /gene="ahpF"
                     /locus_tag="KLK84_003867"
     CDS             complement(1032..2597)
                     /gene="ahpF"
                     /locus_tag="KLK84_003867"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBE2011005.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(2842..3405)
                     /gene="ahpC"
                     /locus_tag="KLK84_003868"
     CDS             complement(2842..3405)
                     /gene="ahpC"
                     /locus_tag="KLK84_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HBE2011006.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            3777..4523
                     /gene="dsbG"
                     /locus_tag="KLK84_003869"
     CDS             3777..4523
                     /gene="dsbG"
                     /locus_tag="KLK84_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HBE2011007.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            4733..5635
                     /locus_tag="KLK84_003870"
     CDS             4733..5635
                     /locus_tag="KLK84_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE2011008.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            5782..5981
                     /locus_tag="KLK84_003871"
                     /pseudo
     CDS             5782..5981
                     /locus_tag="KLK84_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         1605 a         1440 c         1347 g         1589 t
ORIGIN      
        1 tgacgaacgg aattaccacc cggtcgcctt tttgcaaatt ttttacgtcc ttcccggttt
       61 caacgacttc ccccataaat tcatgaccaa aaatatcgcc atgtttaacc tggggtattt
      121 tgcctcgata aagatggagg tcagagccac agatcgccgt taccgtaata cgcagaataa
      181 tatcatctgc ctgttcaatg cccggatcgg gaacattttc tacctgaacg tgatgtgggc
      241 cgtgataagt caatgctttc atgcaacctc cagagacagt tcaaaaaaga gtcagtcttt
      301 aagggtagct atcacagcgg gaaaacgggc ggtaccgcag gttttgggag aattcctagt
      361 gttcagtgag aatatattaa tcacagaaac cgttcgtaag gaaaaagaac cagacaaata
      421 tcagggataa atattataat catggagaat gatttgttca tgattaacag ggagaaaggg
      481 ttatgtataa ggcaatcatt atgccagttg atgtatttga aatggaattg agcgacaaag
      541 ctgttcgcca cgctgaattc ctcgcccagg atgacggagt tattcatcta cttcacgtac
      601 tacccgggtc agccagcctg agcctgcacc gttttgccgc tgatgtgcgt cgttttgaag
      661 agcatctgca acatgaagca gaagaacgtc tgcaaacgat ggtcagccac ttcaccatcg
      721 atccttcccg cattaaacaa catgtccgtt ttggtagcgt gcgggatgaa gtcaatgagt
      781 tggcagaaga actgggggct gatgttgtag ttatcggttc tcgcaaccca tcgatttcga
      841 cccatctgtt aggttctaac gcctcgagcg tgatccgcca cgccaatctg cctgtactgg
      901 tcgttcgcta acagataaaa gtatttatgc cgccctgcct ggaacatccc ggcagggctt
      961 tttaatgcca taaaaaagcc gccaggtttg accccggcgg ctaagcaatt gcaggtgaat
     1021 cttacttctt cttatgcagt tttggtgcga atcaggtagt caaaagcact cagagaggct
     1081 ttggcacctt cgccagtggc gatgatgatc tgcttgtacg gaaccgtcgt acagtcaccc
     1141 gctgcgaaca cgcctttgac gttggtttca catttcgcat caatgataat ctcgcccatg
     1201 cggttacgtt cgactgcgcc ttccagccag ttggtgttcg gcagcagacc aatctggaca
     1261 aaaataccgg ccagttcgat gttgtgaata ttgccgctga cacgatctcg atattccaga
     1321 ccaacgactt tgctgccgtc gcctttcact tccgtggttt gcgcattcag aataatgtcg
     1381 acgtttttca ggctgcgcag tttgtcctgc agaacctggt cggctttcat ttctggcgca
     1441 aattccagca gcgttacgtg ctcaacgata cccgccaggt cgattgccgc ttccacgccg
     1501 gagttaccgc cgccgataac cgctacgcgt ttgcctttaa acagcggtcc gtcgcagtgc
     1561 gggcagtagg tcacgccttt agtgcgatac tgatcttcgc ccggaacgtt catgttgcgc
     1621 cattttgcac ccgtcgccac gataatgctg cgtgctttca gtaccgcgcc agaagctgtt
     1681 tcaatctggt gtagaccacc ttcaaccgct gccgggatca gtttgctggc gctctggctg
     1741 tcgatcacat caacgtcgta ttcatcaacg tgaactttca gtgcgcctgc caacttctgc
     1801 ccttcagtct tcggtacaga aatgtagttt tcgatatcaa cggtatcgag gatctgacca
     1861 ccaaaacgtt cgcccatcag accggtacgg atgcctttac gtgcggagta aattgctgcc
     1921 gctgcacccg ccgggccgga accaacgatt aatacgtcat aagcatcacg cttgttcagc
     1981 tcttctgccg cacgtttttc cgcgccagta tcaattttgg caacgatttc agtcaacgtc
     2041 atgcggccct gaccaaactc tttcccgttt acgaacactg ccggaacgcc catcacgttg
     2101 cgatcggtga tttcgttctg gaaggtgccg ccgtcaattg cagtgtgctt gatgcgcggg
     2161 ttcaatacgc tcatcaggtt cagcgcctgc accacgtccg ggcagttatg gcaagagagc
     2221 gagtaatagg tttcgaattc aaaatcaccg tcaatatggc gaatctgctc cagcagagac
     2281 tgcgcttctt tcgacggatg accaccggtc cacagcaacg ccagtaccag cgaggtgaac
     2341 tcgtggccca gcggggagcc tgcaaaacgt ggtccctggt tggaacctgg gttggtgatc
     2401 aggaaagacg gcttacgcac tggcaagctg ttatcttctt taaaggtgac tttgtctgac
     2461 agttctgcga tttcagccaa cagttccttg atttctgccg atttagcgct gtcatccagc
     2521 gtggcaatta actcaacagg cttggtcaat ttctcaaggt aagccttgag ttgagttttc
     2581 atatttgtgt cgagcatgtt tatctcctgg gctttaaaca tcatcatgca agcggcctct
     2641 gcggctacct ggatgcagct tgcatcatgg tgcggggaag aaaatcgggt gccgcagcac
     2701 ccgaagaatt aggtgaattt ccgaagtatt tcgcgtcaga gcaaggcgac tagcctgtga
     2761 atccccggga gcttacacaa gtaagtgacc ggggtgagcg ggcgacgcca acgccgctat
     2821 ggcgtgaaag acgaaggaaa tttagatttt accaaccagg tccagagacg gagccagagt
     2881 tgcttcacct tctttccatt tagccgggca aacttcacct gggtgagaag ctacgtactg
     2941 agctgctttg attttacgca gcagatcaga cgcgtcacgg ccaatgcctt cagcggtaac
     3001 ttcgattgcc tggatgatac cctgcgggtc aacaacgaag gtcgcacggt cagccagacc
     3061 ttcatcttca cgcatgttgt cgaagttacg ggtcagggcg ccagtcgggt cgccgatcat
     3121 cgcatatttg attttagcga tggtttcaga gctgctgtgc catgctttgt gggtgaagtg
     3181 agtatcggta gatactgcgt atacgtctac gcccagtttc tgcagttctt cgtagtggtc
     3241 agcaacgtca cccagttcgg tcgggcatac gaaagtaaag tcagccgggt agaagaagaa
     3301 gacgctccag cggccttcgg tatctttttc ggtgatttcg atgaattcgc cgtttttgaa
     3361 tgcctggttt ttaaaaggtt taattttggt gttaatcaag gacatctata cttcctccgt
     3421 gttttcgatg agatgtaagg taaccaattt ttgccgattc ggctaatgcg tttccattat
     3481 caaatcgata agttttacct tacaaccttc gtaagacaac tttgtgaact ttgcaccgcc
     3541 agcggcgata aaaagtaaaa tggccccctt tccaggtgac cgtgtaatct gaactaccct
     3601 ttggtatctt cagcgcctgc ctgcaggctg aggtgttgcc cttacaaatg caacaacgac
     3661 atggattaca acaccctcat aaacaaaggg caatcacctg atctaagctc ttacctatga
     3721 cagtgatagg ttatgccttt tactcgactt ttgcactgac tgaaaagaac aaattaatgt
     3781 taaaaaagat acttttactg gctctgcttc ctgcaatcgc cttcgcagag gaacttcctt
     3841 ccccagtaaa agcgattgaa aaacagggca ttacaatcat caaaacgttc gatgcccccg
     3901 gaggaatgaa aggttatctc ggaaagtatc aggatatggg cgtcaccatc tacctgactc
     3961 ccgatggtaa gcacgctatc tctggttaca tgtacaacga gaaaggtgaa aacctgagta
     4021 acacacttat cgaaaaagaa atttacgcac cagccggacg cgaaatgtgg caacggatgg
     4081 aacaatccca ctggctcctc gacggtaaaa aagatgcgcc ggtcattgtc tacgtcttct
     4141 ccgatccgtt ctgcccatat tgtaaacagt tctggcagca ggcgcgcccg tgggtagatt
     4201 ctggcaaagt gcaattaaga acattgttgg ttggggttat caagccagaa agcccggcga
     4261 cagcagcggc aattcttgcc tccaaagatc ccgcaaaaac ctggcaacaa tatgaagcct
     4321 ctggttgcaa gcttaagcta aacgtgcctg caaacgtaag tacagagcaa atgaaagtgt
     4381 taagtgacaa tgagaaactg atggacgatc tgggggcaaa tgtcacgccg gctatctatt
     4441 acatgagtaa ggaaaatacg ctgcaacagg ccgtggggtt gcccgatcag aaaacgctta
     4501 atatcattat gggaaataaa taaaaggtaa ttcatggatg gttgaactat atccctgctt
     4561 ttcaaccatc ctaaaaaagc atgaatttct cgctctaccc taagtggctg tgctatttta
     4621 gaaaaactta atcctgagtt aagcaaaaaa acataatcaa taaaaataca tacgcaaaaa
     4681 acattgacta agtgaatata ccatggaaga aaaatataaa ccggagtagt gtatggccaa
     4741 tctctacgac ttgaaaaagt tcgacttaaa tcttcttgtc atatttgagt gtatttacca
     4801 gcatttaagc attagcaaag ctgctgaatc actatatatc accccatcag ccgtgagcca
     4861 gtcgttacaa cgcttacgtg cgcaatttaa tgacccttta ttcattcgct caggaaaagg
     4921 tatcgcgccg acgacaacag ggttgaatct gcatcaccat cttgagaaaa acctcaaagg
     4981 ccttgaacaa acaattaata tagtcaataa gtccgaacta aagaaaaact ttattatcta
     5041 cggccctcag ctcatttctt gctctaataa tagtatgctg atccgctgcc tacggcaaga
     5101 tgcctctgtc gagattgagt gccatgatat cctcatgtca gcagagaatg ctgaagaact
     5161 gttggttcac cgcaaagccg atctggtgat cacccaaatg ccagtgatca gccgttccgt
     5221 catttgcatg ccattgcata caattcgcaa tactctaatt tgtagtaata agcatccgcg
     5281 aattactgac aatagcactt atgaacaaat tatggcagaa gagttcaccc aattaatagc
     5341 caaatcagca ggagttgatg atattcaaat ggaaatagac gagaagttta tgaatcgcaa
     5401 aatttcattt cgtggttcct ctttgctgac aataattaat agcattgcag ttacagattt
     5461 actgggaata gtcccctatg aactttataa ctaccaccgt gactttctta atttaaaaga
     5521 gataaaacta gaacatccgt taccttcaat aaagctttac atttcctata ataaatcttc
     5581 tttaaataat ttggtgttct cccgatttat tgaccgtcta aatgagagtt tttgatatat
     5641 aactatagac agctactata actttcatct atttattcac agcgcgaatg aaaaataaaa
     5701 cactctgctt tacagtgttt actttataat taacagtctt atctcactgt tcaccctgta
     5761 acgttcagag agcgtcttca gatgtctatt tataaaattc ctcttccgct caatattctg
     5821 gaagctgcac gagagcgtat tacctagaca ctcaacaccc taccccgcgt ttgtgtctca
     5881 ttttctggcg gcaaagactc tggtttaatg ctgcatctga cagccgaact tgcccgacaa
     5941 atgggcaaaa aaatctgcgt tttgtttatc aactgggagg c
//
LOCUS       DAEEUE010000170         5868 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.170, whole genome shotgun sequence.
ACCESSION   DAEEUE010000170 DAEEUE010000000
VERSION     DAEEUE010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5868
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.170"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            11..670
                     /locus_tag="KLK84_003872"
     CDS             11..670
                     /locus_tag="KLK84_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HBE2011009.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(871..1248)
                     /locus_tag="KLK84_003873"
     CDS             complement(871..1248)
                     /locus_tag="KLK84_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE2011010.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1315..4281)
                     /locus_tag="KLK84_003874"
     CDS             complement(1315..4281)
                     /locus_tag="KLK84_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HBE2011011.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(4284..4844)
                     /locus_tag="KLK84_003875"
     CDS             complement(4284..4844)
                     /locus_tag="KLK84_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBE2011012.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(4970..5320)
                     /locus_tag="KLK84_003876"
     CDS             complement(4970..5320)
                     /locus_tag="KLK84_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HBE2011013.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(5523..>5868)
                     /gene="intI1"
                     /locus_tag="KLK84_003877"
     CDS             complement(5523..>5868)
                     /gene="intI1"
                     /locus_tag="KLK84_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HBE2011014.1"
                     /translation="GHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQ
                     AGITKPATPHTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRS
                     PLDALPPLTSER"
BASE COUNT         1162 a         1638 c         1783 g         1285 t
ORIGIN      
        1 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
       61 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
      121 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
      181 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat
      241 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
      301 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
      361 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
      421 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
      481 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
      541 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
      601 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
      661 cggggcgtaa tttttttaag gcagttattg gtgcccttaa acgcctggtt gctacgcctg
      721 aataagtgat aataagcgga tgaatggcag aaattcgaaa gcaaattcga cccggtcgtc
      781 ggttcagggc agggtcgtta aatagccgct tatgtctatt gctggtttac cggtttattg
      841 actaccggaa gcagtgtgac cgtgtgcttc tcaaatgcct gaggccagtt tgctcaggct
      901 ctccccgtgg aggtaataat tgacgatatg atcatttatt ctgcctccca gagcctgata
      961 aaaacggtta gcgccggggt tggatttttc agcgttccag ctaaggctaa ggcattcctg
     1021 ttcaagcgca agccgggcta taaagcgcat tatcgcttta cccgtgccct tatttcgatc
     1081 gcactgagaa acatacagct ctttaatatg cagctgaccg ctgtatcggg gcgagggata
     1141 aagaatattg caacatgcca ggccagtaat gttattgccg cagcgcgctc tgatcaccag
     1201 ggtaccggaa agccggttga ataacttttt acagagataa tccttcatca acgcttcctg
     1261 aatgatacct tcgccataat aggggtcgtc tcagaaaacg gaaaataaag cacgctaagc
     1321 cggttgcaga ggccgtagcg gcctgaactt ccccgcgccg atcttggcgc tgctgcgcca
     1381 taggtaatca ccggtcaggt tgatgtgctc ccagccgagt ggcgacaggt actgcaatag
     1441 cgagtcatcg acggcatgac cattgccgcg caacgcatgc gccgcacgct ccaggtagac
     1501 cgtgttccac agcacgatgg ccgccgtcac caggttgagg ccgctggccc ggtagcgctg
     1561 ctgctcgaaa ctgcggtcac ggatttcacc aaggcggttg aagaacacgg cacgggccag
     1621 cgcattgcgc gcctcgccct tgttcagccc ggcatgcacg cggcggcgta gctcgacgct
     1681 ttgcagccag tcgaggatga acagcgtgcg ctcgatgcgg cccaactcgc gcagcgcgac
     1741 ggccaagccg ttctggcgcg ggtagctgcc gagtttcctg agcatcagcg aggccgtcac
     1801 cgtgccctgc ttgatcgagg tggccagccg caggatttcg tcccaatggg cgcggacgtg
     1861 cttgatgttg agcgtgccgc cgatcatcgg cttgagcgcg tcataggcgg catcgccctt
     1921 cgggatgtag agcttggtgt cgcccaggtc gcggatgcgc ggcgcgaagc ggaagcccaa
     1981 gaggtgcatc agggcgaaga cgtgatcggt gaagcccgcc gtgtcggtgt agtgctcctc
     2041 gatccgcagg tcggattcgt ggtacagcag gccgtcgagc acgtaggttg agtcgcgcag
     2101 gccgacattg accaccttgg tgtggaatgg cgcgtattgg tcggagatgt gggtgtagaa
     2161 agtccgtcct gggctgctgc catattttgg gttgatgtgc cccgtgctct ttgccttgct
     2221 agcggttcgg aaattctgtc cgtccgatga tgatgtggtg ccatcgcccc agtgcccggc
     2281 aaagggatgc cgaaactgag cgttgaccag ttcagccaac gctgtcgagt acgtttcgtc
     2341 gcgggtatgc caggcttgca gccaagcgag cttcgcgtag gtcgtgccgg ggcaggactc
     2401 ggccatcttg gtcaggccca ggttgatcgc gtcggccagg atcgtggtca acaacaggtt
     2461 cttgtccttg gccagatcgc ccgatttcaa gtgcgtgaag tgccgggtga agcccgtcca
     2521 ctcatcgact tcgagcagca gttcggtgat cttgacgtgc ggcaggacca tggctgtctg
     2581 gtctatcagc gcctgcgcgg tgtcgggcac cgccgcatcc agcggcgtga tcttcaagcc
     2641 cgactcggtg atgatggcat ccggcaggtc gttggctgcc gccatgcggt tgacggtggc
     2701 aagttgtgct tccagcagcg tcagccgctc atgcagatat tgttcgcagt cggtggccac
     2761 ggccagcggc aattcgctgg actgcttgag gctggtgaac ttctcgggcg gtaccaggta
     2821 gtcctcgaag tccttgaact ggcgtgaacc ctgcacccag atgtcgcccg agcgcaggga
     2881 gttcttcaac tcggacagcg cgcacagttc gtagtagcgc cggtcgatgc cggcgtcggt
     2941 catcaccagt ttctgccagc gcggcttgat gaagccggtc ggtgcatcgg ctggcagctt
     3001 gcgggcgttg tcggtgttca tgccgcgcag cacctcaatg gcatcaagca cgtttttggc
     3061 ggcgggcgcg gcccgcagct tgagcacggc aaggaattcc ggtgcatagc ggcgcagggt
     3121 ggcgtagctc tcgccgatgc gatgcaggaa atcgaagtca tcgggttgcg cgagcttctg
     3181 cgcctcggtg acgctctcgg caaaggaatc ccaggacatg acggcctcga tggcggcaaa
     3241 cgcatcgcgg cctgattgct tggcgtcgat cagcgcctga ccgatgcgcc cgtacagacg
     3301 taccttggcg ttgatggcct tgcctgacgc ctggaactgc tgctgatgct tattcttggc
     3361 agcgttaaac agcttaccca ggatgcggtc gtgcaggtcg atgatttcgt cggtgacggt
     3421 ggccatgccc tcggtggcca gcgccacgag agtggcgtag cgccgttgcg gctcgaattt
     3481 ggccaggtcg gcgggtgtca tctggccgcc ctcgcgggca atcttgagca ggcggttctg
     3541 gtgaaccagc cgctcgatgc cggtaggcag atcgagtgcc tgccatgcct tgaggcgttc
     3601 gatgtgttcc agcatatgcc gcgaatttgg cttggccgga gactggcgca accaagccaa
     3661 ccaggtcgtc ttgccgttgt cccggcgctt gagcagatcg tcgaggcggc ggcgatgcgc
     3721 gtccgccagt ggttcggcca aggcgtcgta gatgcgccgg ttagcacggg tgatcgcctc
     3781 ggcactcgcc cgctcgacgg cgttgagggc gggcagaatg accgactgcc gccgcaggtg
     3841 cccgatcaag gcgctggcca gcacgatgcc tttgtcggtt tgcatcgcca gctcggtcag
     3901 catctggacg gcctgccggt aatggctcat ggtgaagggc cggaaaccga acacggtttg
     3961 cagctcgctc aggtgctcgc gccgggtctg ctcccgctgg ccgtactcgt tccagctttc
     4021 gacgccgacc ttgagctggt cggcgaccag cttcaacaag ggcgggaacg gtagttcatc
     4081 gacgcccagg atgacgccgg gaaagcgcag gtaacagagc tgcaccgcga agcccagccg
     4141 attggctggc ccgcgccgct gtcggatgat cgagaggtcg gtatcgttga atgtgtaatg
     4201 tcggatcagg tcgtccttgg agtccggcaa cgccagcagg ctttcccgct cggcggcgga
     4261 caggatggaa cgacgtggca tatttactga tccgttctca agtattgata cagggtttcg
     4321 cgactgattc cgaattcacg agcaagcttg gtcttttgct cgccagcctc gacacgttgg
     4381 cgcagttcgg caatacgctc agacgacagg gatttcttcc tgccacggta agccccgcgt
     4441 tgcttggcga gcgcaatacc ctcgcgctga cgctcgcgga tcagggcgcg ctcgaactcg
     4501 gcgaacgcgc ccatcaccga gagcatcagg ttcgccatcg gagagtcttc gccagtaaaa
     4561 ctgaggtgtt ccttgacgaa ttcgatatgc acgccgcgtt gtgtcagcgt ttgcacgatc
     4621 cggcgcaaat catcgagatt gcgcgccagg cgatccatgc tatgcaccac cacggtgtcg
     4681 ccggtgcggg cgaagcttat cagcgcttcc agttgcggac gcttgacatc cttgccggat
     4741 gccttgtcgc taaaagcgcg atcaaccttg acgccttcca gttgccgttc cgggttctgg
     4801 tcgaaggtgc tgaccctgat atacccaatg cgctgtccag tcatggaatt ccctgcaaaa
     4861 tgtcagggaa gactctatga ccttcaacga gatatgtcaa taaattcaaa attcaatcct
     4921 atcctgacgc aatttacaca tggcatctga catcaggtta gggtatgcct caacctgacg
     4981 gcggcgaatc acaagcgtcc ggtttgacgc tggtttcggt cgcagtgctg gcccgcgcct
     5041 ggaagcgctg atagcactcc agcccgcaga aatgctccac gtactcggcc ccttccggcg
     5101 tgaaggcggc atcgagcggg atttccttgc agcacacgca gcaactggtg ctcggttcat
     5161 tggcgttcat ggtggtgttc ctccattggt tgacgaagcc gacgaaggcc gccgccggca
     5221 tcggcctggc gaacaggaaa ccctgcaccg tgtcgcaacc cgcctgccgc aaccacgcaa
     5281 ggcagtcggg tgtttccaca ccctcggcta ccacctccat tgtcgttttc agaagacggc
     5341 tgcactgaac gtcagaagcc gactgcacta tagcagcgga ggggttggat ccatcaggca
     5401 acgacgggct gctgccggcc atcagcggac gcagggagga ctttccgcaa ccggccgttc
     5461 gatgcggcac cgatggcctt cgcgcagggg tagtgaatcc gccaggattg acttgcgctg
     5521 ccctacctct cactagtgag gggcggcagc gcatcaagcg gtgagcgcac tccggcaccg
     5581 ccaactttca gcacatgcgt gtaaatcatc gtcgtagaga cgtcggaatg gccgagcaga
     5641 tcctgcacgg ttcgaatgtc gtaaccgctg cggagcaagg ccgtcgcgaa cgagtggcgg
     5701 agggtgtgcg gtgtggcggg cttcgtgatg cctgcttgtt ctacggcacg tttgaaggcg
     5761 cgctgaaagg tctggtcata catgtgatgg cgacgcacga caccgctccg tggatcggtc
     5821 gaatgcgtgt gctgcgcaaa aacccagaac cacggccagg aatgcccg
//
LOCUS       DAEEUE010000171         5784 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.171, whole genome shotgun sequence.
ACCESSION   DAEEUE010000171 DAEEUE010000000
VERSION     DAEEUE010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.171"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..941)
                     /gene="hprS"
                     /locus_tag="KLK84_003878"
     CDS             complement(<1..941)
                     /gene="hprS"
                     /locus_tag="KLK84_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HBE2011015.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLN"
     gene            complement(941..1612)
                     /gene="hprR"
                     /locus_tag="KLK84_003879"
     CDS             complement(941..1612)
                     /gene="hprR"
                     /locus_tag="KLK84_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HBE2011016.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            1745..2158
                     /gene="hiuH"
                     /locus_tag="KLK84_003880"
     CDS             1745..2158
                     /gene="hiuH"
                     /locus_tag="KLK84_003880"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HBE2011017.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2267..3271
                     /gene="msrP"
                     /locus_tag="KLK84_003881"
                     /pseudo
     CDS             2267..3271
                     /gene="msrP"
                     /locus_tag="KLK84_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            3272..3907
                     /gene="msrQ"
                     /locus_tag="KLK84_003882"
     CDS             3272..3907
                     /gene="msrQ"
                     /locus_tag="KLK84_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HBE2011018.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            4165..4815
                     /gene="zinT"
                     /locus_tag="KLK84_003883"
     CDS             4165..4815
                     /gene="zinT"
                     /locus_tag="KLK84_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HBE2011019.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         1643 a         1305 c         1196 g         1640 t
ORIGIN      
        1 ttattgagag aaagcgaatc cagtttcacc aaaacattgt ttttgtctgc cctggcaaga
       61 aacagtatat tctctgttaa ccgcgaaata ttttccagct cttcaatgtt tccggcaatt
      121 gttttttgat attcagcgat acttctgatt tgactgagcg taacctgatt ctgacccagt
      181 aatgcattaa ttggcgttct aagttcatga gcgagatcgt cagcaaattg actaaggcgc
      241 tcaaaatctt tgactaaagc atgatgcatt ttattcaacg cctgcccaag aggttttagt
      301 tctctcggta atgcgttaac ctcaaccggc actcggctat cgttataatt cagcgcttcc
      361 gttacgccac tcaacttttt gatctctcgc aaccccgtcc tgattaacaa cggacttaat
      421 actgagcaca gtataatggc gacaatgcaa attataatgc tattaatttt atactgttca
      481 agcatattat gtctggctga agccaattta gccacagtaa ccgttaatga tggcgaaact
      541 tcatcaatgt taattcgtaa agcatctatc tctgtatcat taataatgct tctgtaaatg
      601 cctgctgcgc tgattgtctc actagcaggt atgttattta acatgtcatc actgacattt
      661 gtccggttaa caattttatt gatgccatca ctatgaatga tcaaaatatc ctgactaaca
      721 tccatcatcc ggttaaagta cacaggtaac gtatctggat ttaccccatc aattaacaac
      781 tgcttgatct gcgctgtccg gttaatgagt gttgtatcat cgcgccattt caactcactt
      841 gccaggccat tatagagagt ccatacaatt ccagcgccag caacagactg cagaaatata
      901 aaaagcaagg ttaaacggac ggttatagat aatcttttca ttattttttt accgctacga
      961 atgaatagcc catcccccgg attgtggcga ttagcttttc aggaaaagga tcatcaactt
     1021 ttgcgcggag cctgcgaatg gcgacatcca ccgtattggt atcactatca aagttgattc
     1081 cccaaatttc actcgcaata accgttctgg gtataatctc gccagctctg gaggccagta
     1141 gccaaagtaa ctgaaactcc ttgcgcgtca gtgtaatact gatattgtcc ctgctgacac
     1201 tttgactgac agagtccatt tttaagccac tgatttctaa tgttgaattc agagcgtgat
     1261 gttgccttaa ttgtgcccga acccttgcta gcaactcaga aaatgaaaaa ggttttacca
     1321 gataatcatt tgccccactg tccaaccctc tgactctgtc atcgacagaa tcccttgcag
     1381 taaggcaaat aacaggggtt tgctttgccg ttcttaacgt ttgtaagatc tgccagccat
     1441 ccatacccgg aagcataata tccagaatga tcaatgcata atcatccttc agcgcaagat
     1501 aaagcccatc tctgccatca gaaacggcat caatgacata acccgcttcg gaaagcccct
     1561 gcgttaccca ttcctgggtc ctttgattat cttcaataag tagaatcttc atgaaaataa
     1621 tatgccataa atcatatgtt atagaaacaa cctggttcat tacaaaattg taatgctgct
     1681 gtaaggttac cctggacgtt ttttcgctat cctcaaaact cattcacatg acaaggatat
     1741 aaacatgtta aagcgttatt tagtactctc ggtagcaacg gcagcatttt cattaccttc
     1801 tttggtttat gccgcacaac aaaacattct tagcgtgcac attttgaacc agcaaacagg
     1861 aaaacccgct gccgacgtga cagtcactct tgaaaagaaa gcggataacg gctggttaca
     1921 acttaatacc gccaaaacag ataaggatgg acgaattaag gcactgtggc ccgagcaaac
     1981 tgcgactact ggcgattacc gtgtcgtatt taaaaccggg gactatttca agaaacaaaa
     2041 tcttgaaagt ttcttccctg agattcccgt tgaatttcat attaataaag tgaacgagca
     2101 ttatcatgtg cctttgttac tcagccaata tgggtattca acctatcgtg gcagttaatg
     2161 cgtaaatatg taaaagctga tctctgctac tgttagggag gtgactccct gacagattaa
     2221 cagtaaacag ctcttgttgg ttaacgacaa aaaagtgtga tggcttatga aaaagaagca
     2281 atttttaaaa gaaccagatg ttacggccga gtcggtattc tttatgaagc gtcggcaggt
     2341 gttaaaagca ctgggcatca gcgcagctgc attttctttg cctcacgctg cgcatgccga
     2401 tctgcttagc tggtttaaag ggaacgatcg tccgcccgcc cccgccggaa aaccgctgga
     2461 gttcagcaag cctgccgcct agcaaaataa cctgccactg acgccagcag ataaagtctc
     2521 cggttataac aacttctatg aattcgggct ggataaagac gatcccgcgg ctaatgctgg
     2581 tagcctgaaa accgatccat ggacactgaa aatcagcggc gaagtggcaa aaccattgac
     2641 cctcgatcat gatgatttaa cccgtcgctt cccgctggaa gagcgtattt atcgtatgcg
     2701 ctgcgtggaa gcgtggtcga tggtggtgcc gtggattggt tttccgctac acaaattact
     2761 ggcgcttgcc gaacccacca gcaatgcgaa gtatgtcgct ttcgaaacga tttatgcgcc
     2821 ggaacagatg cctggccagc aggaccgctt tatcggcggc gggctgaaat atccttatgt
     2881 cgaaggattg cgtctcgacg aagcaatgca tccgctcaca ctgatgaccg taggtgttta
     2941 tggcaaggcg ttaccaccgc aaaatggcgc gccggtgcga ctgattgtgc cgtggaaata
     3001 tggctttaaa gggattaaat cgatcgtcag tattaagctg acccgcgagc gtccgccaac
     3061 cacctggaat ctggcagcgc ctgacgaata cggtttttac gccaacgtta atccgcatgt
     3121 tgatcacccg cgctggtcac aggctaccga acgatttatt ggttcaggcg gcattctcga
     3181 tgttcagcgc cagccaacac tactgtttaa tggttacgcc gaccaggtgg catcgctgta
     3241 tcgtggcctg gatttgcggg agaatttcta aatgcgtctg acagcaaaac aagtgatatg
     3301 gctgaaagtt tgcctgcatc ttgccggatt gttgccgttt ctctggctgg tctgggcgat
     3361 caatcacggt ggactgggtg ccgatccggt gaaagatatt cagcatttta ccggtcgcac
     3421 tgcactgaaa tttttgcttg cggccttgtt aatcacacct ctggcacgct acgcaaaaca
     3481 gccgttattg atacgcactc gccgcctgtt aggcttatgg tgctttgcct gggcgacact
     3541 gcatttaacc agttacgcat tgctggagtt gggcgtgaat aatctggcgt tattaggtaa
     3601 ggagttaatt acccggcctt atttaacgtt aggaattatc agctgggtta ttttgcttgc
     3661 tttagcgttc acttcaaccc agtcgatgca gcgaaaactc ggcaaacact ggcaacagtt
     3721 gcataacttc gtctatcttg tcgcgatcct ggccccaata cattatctgt ggtcagtgaa
     3781 gattatttca ccgcagccac tcatctacgc tgggctggct gtactgcttt tagccttacg
     3841 gtataagaag ttgctttccc ttttcaaccg gttacgcaag caggcccaca acaaattatc
     3901 tctataactt catattgccg acaaacttcg cgttatcccg gccggatgcg gcgcgagcgc
     3961 cctatccggc ccacacagat cgcatcaagt tatgtttcgt ttgtcatcaa cctttcatca
     4021 ccagaataat ttattttatt tcaataagat agttcaatac acctgagaat accatgctct
     4081 catttcctaa tgagttgttg cattttttca tatgttacaa tataacatta cacataatgt
     4141 acattaactc tggaggaaat tgttttggcg attcgtcttc ataaactggc tgttgcttta
     4201 ggtgtcttta ttgttagcgc tcctgccttt tcgcatggtc atcattctca tggcaaaccc
     4261 ttaacagagg tcgaacaaaa agctgctaat ggtgtttttg atgataccaa tgtacaaaac
     4321 cgaacgctca gtgactggga tggagtctgg caatccgttt accctttact gcaaagtggc
     4381 aaacttgatc ccgtctttca gaagaaagcg gatgcagata aaaccaaaac atttgctgaa
     4441 attaaagatt attatcgcaa aggttatccg acagatatcg aaatgattgg cattgaggac
     4501 ggcattgtcg aattccatag aaataatgaa acaacatcct gtaaatatga ttacgatgga
     4561 tacaaaatac tcacctataa atcaggcaag aaaggcgttc gttacttatt cgaatgtaaa
     4621 gatcctgaaa gcaaagcccc taaatatata caatttagcg atcatataat tgcaccacga
     4681 aaatcatctc attttcacat ttttatgggt aatgattccc agcaatcttt attgaatgaa
     4741 atggaaaact ggccaacgta ttatccctat cagttgagta gcgaagaagt ggtcgaggag
     4801 atgatgtctc attgattttt ttgcgctgac ttcagttatt taacagaaca gtgcctggta
     4861 gtaacaacaa taatttacca agacaaataa gtaagacaaa ctcagacacg ctcatacttg
     4921 agtcgtgtct tttttattat gtctgtagaa aaaaactgag aaaaaggcta acaataaata
     4981 cacaactcag ccatacagac tggcagcgca aatgtttcca ggagcaagtc atctgtcatg
     5041 gtaaagctaa ccagaaatat aggttaattt tggtgtttaa acctttttta cttgttagta
     5101 tgatacaaag gctttcaaaa aagctgggaa attcaacaac tcttggaaat tatggacttt
     5161 gtcccgcggc tttaatctga cggccgcgtt ccttttttgg ctcaattatt gaccccttct
     5221 ctacgcaact tcagttcccg ccagcaactt tgcggcagtt tcataggatc aatgtctaaa
     5281 agaataatgg tgaccgataa gaaaacgact gaataactgt agattttcgc tcgaaacctt
     5341 cctgtcagct ccatagcgaa tcaagcgctg aatgtcacag tatcgaacag aaaacagtga
     5401 cgatctaacc cttcaagaat attctacgat tgttctgttt aggaaaagca aggcgggaag
     5461 tcgggagata agtcattgat aaaatggcgg agagaggggg atttgaaccc ccggtagagt
     5521 tgcccctact ccggttttcg agaccgatga tacagagtcg aaaaatcaaa gagttaaaaa
     5581 tttttcttgg aaaaaatgtc ttaaaatcgt acacgaaaaa tcaatgaatt acataacatt
     5641 aaagaagaat attccaagcc ttcttgagtc caccacctgt tttcaggtgt cattcagacg
     5701 tgatttgccc cagattggga aagcacgctt ttttctcagc ttattccgtt ttgcctgtgt
     5761 gttgaataat tccactgcag ttat
//
LOCUS       DAEEUE010000172         5758 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.172, whole genome shotgun sequence.
ACCESSION   DAEEUE010000172 DAEEUE010000000
VERSION     DAEEUE010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.172"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..402)
                     /locus_tag="KLK84_003884"
     CDS             complement(<1..402)
                     /locus_tag="KLK84_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
                     /protein_id="HBE2011020.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTAL"
     gene            complement(547..1050)
                     /gene="cheW"
                     /locus_tag="KLK84_003885"
     CDS             complement(547..1050)
                     /gene="cheW"
                     /locus_tag="KLK84_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBE2011021.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(1071..3035)
                     /gene="cheA"
                     /locus_tag="KLK84_003886"
     CDS             complement(1071..3035)
                     /gene="cheA"
                     /locus_tag="KLK84_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBE2011022.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(3040..3966)
                     /gene="motB"
                     /locus_tag="KLK84_003887"
     CDS             complement(3040..3966)
                     /gene="motB"
                     /locus_tag="KLK84_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HBE2011023.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(3963..4850)
                     /gene="motA"
                     /locus_tag="KLK84_003888"
     CDS             complement(3963..4850)
                     /gene="motA"
                     /locus_tag="KLK84_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HBE2011024.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGVLIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(4977..5555)
                     /gene="flhC"
                     /locus_tag="KLK84_003889"
     CDS             complement(4977..5555)
                     /gene="flhC"
                     /locus_tag="KLK84_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HBE2011025.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRARTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(5558..>5758)
                     /locus_tag="KLK84_003890"
     CDS             complement(5558..>5758)
                     /locus_tag="KLK84_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HBE2011026.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         1341 a         1553 c         1407 g         1457 t
ORIGIN      
        1 taacgctgtg taatagtttt tatatttttc atcaaaatta cggctggtag cgaccatttc
       61 aggtaacggt gccatgcttt tgaatttttt ataatgcgtc gcggcctggg ccaatgtttt
      121 cctagcgcta tcgagcaatt caactttggc gttactttgt tgattggagg aatccatcat
      181 catccgcacc gctgaacgac tcaggttaat gcgcgtttgc agcattaaat cccaggttga
      241 cgtcagctcg ccctgctgtt cccgtaattg attggaaacc acaaagctct tctggctatg
      301 gtgaagggaa gaaaaaaaca aactgccgga aataagctgt aacagtgcga atacccccag
      361 caccattacc aacagcgtga ctacgcggat acggttaatc ataaggcacc ttcctgaaaa
      421 caagttgatc tcgttatcgg caagaagggg ggaaacttta ttgctgatgc cacccgccgc
      481 gaaattgaaa taaaaaaccc gatgcgcaga tcatcgggtt catttcaatt gaggaaatcg
      541 ggagaattac gccacttctg acgccgcgct atctaacagc gccatctctt cgctgttcag
      601 cagtttttcg atgttcacca gaatcaacat acggtcgccc agtgcgccca gtccagtgag
      661 atattctgtt gaaagcgtca cggcaaactc tggtgccgga cgaatttgct ccgccgtcaa
      721 tgaaagcacg tctgaaacgc cgtcaaccac gatgccgacc acccgctgtc cgagattcag
      781 gacgataact accgtgttgt cgttatagtc cacatccacc tggctgaact taattcgtaa
      841 gtcaacaatc ggcacaataa cgccgcgcag attcgtgacg cctttgataa acgctggcgt
      901 gttcgcaatc cgtgttacct gatcgtagcc acggatctcc tgcactttca ggatatcaat
      961 accgtactct tcatcaccaa gggtaaatac cagaaattcc tggcctgacg gctcgctggc
     1021 cagctttgtt acattcgtca taccggtcat attgttacct ttttactcat tcaggcggcg
     1081 gtgttcgcca tacgttgttc gcggtttatc gcctgcaagg cggagacatc aacaatcagt
     1141 gccacgctgc cgtcgccaag aatggtcgca gcagaaatgc cggggacttt gcgatagtta
     1201 ctttcaaggt ttttaaccac aacctggtgt tgaccaatta attgatccac cagcaaggcg
     1261 tagcggcgac cgccactttg taagatcacc acaattccct gggtggcttc ggttttcgcg
     1321 cccgcgacgt tgaacacttt ccacagttcg acgatgggca gatattcacc ccgcacttcc
     1381 agcacccgct cgccgccggc cagtggatgg agatcggctt cacggggttg cagtgattcc
     1441 ataacagcat tcagcggcag aatgaaaact tcatccgcaa cgcgtacgga catgccgtcg
     1501 aggatggcca gcgtcagcgg cagtaaaatg cggatcgtag tgccagtacc ctgcttcgac
     1561 tggatttcga catgaccgcc catctcctgg atattacgtt taacgacgtc catgccgacg
     1621 ccgcgcccgg agacgtcggt gacctgctct gccgtggaga agcctggtgc aaatatcagc
     1681 attgcaactt cgtcgtcgct catgttttcg ctgacagtca aaccttgcga ggccgctttt
     1741 gccagaattc gctcacggtt tagccccgcc ccatcgtcgg tcacttcaat gcaaatgttg
     1801 ccgccctgat gttcggcaga cagaattaaa tttccgacgc tgtttttacc tgcggcgagc
     1861 cgtttttctg gcagttcaat accgtgatcg aggctattgc gtaccaggtg ggtcagcggg
     1921 tcgataatgc gttctatcag gctcttgtcg agctcggtgg aactgcccac cagcgtcagt
     1981 tctacctgct tgccgagttt tcccgccaga tcacgcacca gccggggata gcgactaaag
     2041 acatattcca tcggcatcat gcgaatcgac atcactgatt cctgcaaatc acgggcgtta
     2101 cgttgtaact gccccatgct ggtaatcaaa tcaccatgat taaccgggtc cagttcgctg
     2161 gaacgctggg caagcatgga ctgggtgata accagctcgc cgacgaggtt aattaattga
     2221 tcaacctttt ctaccgctac acggatgctg gtggattcac tgctgcgcgt cgttttttcc
     2281 cgctccacgc ggccggttgg cgcttgttcg gctgccagtt taagcacagg tggggtagct
     2341 atttttggcg aaacttctac tgtttcaaag gtaatctgat cggcttcaat cacaaaacag
     2401 agtaccgctg tgatgtcatc ttcggcgata tcgcccggta atattgccga gagagaatcc
     2461 gcccctttca ccacgtcatt taacgttgtc agatgcccca gctcttcttc cagcaggtcg
     2521 acttcgccgg ccttcaggcg cgaaaggata attcgtcgcg gcaactgact gcgactttgc
     2581 tcatcttgcg gttcactttt ggcaaccaca cttaatcggg tcactgcgga tggcgtttcg
     2641 cctttcgctt ctaatgccag ttgacgcaag gcctggcaga tataatcgta gctggcggca
     2701 tccggctctt gcgactgttt ataagcgtcg agctgttctt gcatgatgtc cttcgtttcc
     2761 aaaaacagat tgataatgtc ggtgttgagt tgcatctcac ctcgtctggc ttcatcgagc
     2821 aggttttcca tcagatgcgt ggtttcctgc aaaacgctga agccaaaagt tcctgcccct
     2881 cctttgatcg agtgggcagc ccgaaagatg gcattcaatt gttcggcatc tggcgcttcc
     2941 ggctgcaaaa ccagcagatg ctgctccata tcagccaaca gttcgtccgc ttcatcaaaa
     3001 aatgtctgat aaaaatcgct tatatcgatg ctcacgctgt cacctcggtt cggctgatgg
     3061 cattgtggga acactgacct gtggtgcaac ctcaggtttt tctagggcgc ttactggctc
     3121 attctggctt tcggcgtttt catgcaaaat ggcctgttcg gcttgtttgt tcagtaccaa
     3181 caggctgatg cgacggttga cggcatcatc aggtccgcga tcgcttaagc gcatcgttgc
     3241 cgccatgccg acgacacgta acactttgcc gctatccaac cctccgacca tcagttcgcg
     3301 gcgggatgca ttggcccgat cggcagaaag ctcccagttg ctgtaacctt tctcaccgct
     3361 ggcgtaggga aaatcatcgg tatgacctga aaggctaata cggttgggaa taccgttcag
     3421 cacaggcgcg atggcgcgca gaatgtcgcg catatagggt tcgacatcgg cactgccggt
     3481 tctaaacatc gggcgattct ggctatcgat gatctgaata cgtagacctt cctggaccag
     3541 atcgattttg agatggagac gtaacgcccg cagtttcgga tcggactcta tcaactggtc
     3601 gagatcaccc cgcaatttcc gcaatcgact ttgctccatg cgttttttca gctcttcgat
     3661 gttcggctgc ttattcactt ccccctggct ctgggtgtaa tcatcaccac cgccgggaat
     3721 tgggctttca ctattggaaa tgcgatcgcc gcccgtaacc gctgtcgcca gtggagtccg
     3781 gaagtactcc gcaatctgaa tcaactcttt tgggctggag atggagatca gccacatcac
     3841 caaaaaaaag gccatcatcg cagtcataaa gtcggcataa gcaatcttcc acgatccatg
     3901 tgctgccccg tggcttttgg ctttgcgtcg tttgacgaca ataatcggat gcgcttgatt
     3961 cttcatgctt cctcggttgt cgtctgttgt tgcggatttt tcaccgcacg cacatgctct
     4021 tccagttcaa taaacgacgg acgttcgctg gaatagagcg ttttgcgacc aaactcaacg
     4081 gcgataggcg gtgcgtaacc gttcagatta gaaagcagag tgactttgac gcactgcatc
     4141 attttgctgg tttcggcgct tttctgacgt aaaacactcg ctagtgggga aataaatccg
     4201 taagccaata aaataccgag gaaagtcccc accatcgcat gtgcgataag cacccccagc
     4261 tcggcggcag gacgatcggc tgaacctaac gcgtgaacgc cccccattac agccgcgaca
     4321 ataccaaacg ccggaagtga gtccccgacc agcgccagac tgttcgccgg gacttctgcc
     4381 tcgctttcgt gcgtctcaat ctcttcatcc atcagggctt cgatttcgaa ggtgttcatg
     4441 tgaccgctga taatcaggcg cagataatcg acgataaaat caagcatgac gctatccgcg
     4501 aggatgcgtg ggtagctggc gaagatctcg ctctcacggg gattttcaat atcacgttcc
     4561 agcgaaaaca tccccatctg ccgcgatttc gccatcaacc gataaagcag agccagcaga
     4621 tccatataca ttgctttggt gtatttggag cgacgaaaca gcaacggcag cgctttcagc
     4681 gtgcctttaa tcgctttgcc attattgccg acgataaacg acccaatccc tgcaccggca
     4741 ataatcacca gttcagcggg ttgatagagt gctccaaggc ttccaccggt catcaaataa
     4801 ccgccgaaaa ctgtaccgag aacaaccagg taacctaata agataagcac gacatcatcc
     4861 ttccactgtt gaccatgaca ggatgttcag tcgtcaggcg ttaacgcgcg attggggaaa
     4921 aaaaaagcag cggtacgtcg ttaccgctgc tggaatgttg cgccacaccg tatcagttaa
     4981 acagcctgta ctctctgttc atccagcagt tgtgggataa tatcggcagg attctgggaa
     5041 agtttacgtc tttttactgc ccgggatggc ggttgacata agctgcaggc aaagctgcca
     5101 acaggctggt gagcgtgggt aataaaattg ccgccgcagc agttgcagct ggaaagttgc
     5161 agtaatccac tttcaacaaa ccgcaccaat gtcctggcac gggttaatgc cagcagtggt
     5221 ccttcttctg cttgtgggca ctgttcaagg tataaacggt aggctttgat caccgcatcg
     5281 acgccattac acaaaccggt tttcagtaaa aactgccatg cattacagaa catcgaagca
     5341 tgaacgtttt gttcccaggt cataaaccag tcggttgaga atggcagcat gcctttcggc
     5401 ggtgggcttc cgcgcagttc tttataaagt tttatcaggc gtccgcgact taactgtgtt
     5461 tcgctttcca gcatctgcaa acgagcgccc agggtgatca attccattgc cagctgaata
     5521 tcccgcgctt cctgaacaat gcttttttca ctcatgatca ggcccttttc ttgcgcagcg
     5581 cttcttcagg ctgattaaca tcattcagca agcgtgttga gagcatgatg ccggtatgaa
     5641 tttgctggag atcgtcaacg cgggaatctt gcgtcaactg agtaatcgtc tggtggctgt
     5701 caaaacggaa gtgacaaacc agctgattgg tttctgccag cttaaccatt tgcggaag
//
LOCUS       DAEEUE010000173         5737 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.173, whole genome shotgun sequence.
ACCESSION   DAEEUE010000173 DAEEUE010000000
VERSION     DAEEUE010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5737
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.173"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..241
                     /locus_tag="KLK84_003891"
     CDS             <1..241
                     /locus_tag="KLK84_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011027.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(238..1179)
                     /locus_tag="KLK84_003892"
     CDS             complement(238..1179)
                     /locus_tag="KLK84_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HBE2011028.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRMVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1191..1680)
                     /locus_tag="KLK84_003893"
                     /pseudo
     CDS             complement(1191..1680)
                     /locus_tag="KLK84_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1684..2904)
                     /locus_tag="KLK84_003894"
     CDS             complement(1684..2904)
                     /locus_tag="KLK84_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HBE2011029.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPCKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2908..3651)
                     /locus_tag="KLK84_003895"
     CDS             complement(2908..3651)
                     /locus_tag="KLK84_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBE2011030.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3542..5008)
                     /locus_tag="KLK84_003896"
     CDS             complement(3542..5008)
                     /locus_tag="KLK84_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HBE2011031.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(5008..5241)
                     /locus_tag="KLK84_003897"
     CDS             complement(5008..5241)
                     /locus_tag="KLK84_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBE2011032.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
     gene            complement(5229..5708)
                     /locus_tag="KLK84_003898"
     CDS             complement(5229..5708)
                     /locus_tag="KLK84_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011033.1"
                     /translation="MDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLTVSEIRLTPLTTENSILWMS"
BASE COUNT         1227 a         1618 c         1435 g         1457 t
ORIGIN      
        1 ctttggcatt ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat acttccgcgt
       61 gcgcacgagt gaaccagtgc gtggcaacgt cttcctccac aacatgacgg cctttaacaa
      121 actcgcgccg agaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg tgtattactg
      181 catcattttt tgccatcggg tcatccttaa tggccccgcc agggggccat gtggctgtta
      241 aatgccatca acgtacgaaa tggtttcttt gtacactggc tcgactgcac ccagcttgcc
      301 gtagtaagtg acgatctgat acagaccgcg atactgcacc ggcacgctct gaagcggaac
      361 cagcgggtag cggacgtatt ttttatcgtt ggtgtacgca accatgcgat ccttattccc
      421 cacaccacgg cctttcagcc atttaaccgc gcggatattc agcggaacac cgttctggtg
      481 atagctgatg gtgttggtct gaaggttcgt caacagggac tggttacctg cagatgaaac
      541 gatgatgctg gacaacagag caaactgctc aggcgggatc agcaaatcac gcggaaccac
      601 agagtaacca gaagcggccc acgcatcaga cagcacctgg ttaatgcttg cgcggatttc
      661 gtccggtgtt gaggttgccc acgttttggc agcgttgttg acaggcacgc cgtccagggt
      721 aacaaggcct ttcaggttta atgcggaatc gccaacatat acctgttcat cgttatccat
      781 ctgccatttc agttgcatac cgtcatactt ctgcgtatca atcgggcggc cgacctgctg
      841 agcagcctgc aattctatga ccgtccagcc aagttccatc ccccacaggt tcagcgggtt
      901 accggatttg ccgatatcca cgttcacgcc agcaatagcg gttgagtctt tgcctaccca
      961 gttttttcca ttcggatttg cgccagtacc cgcagcggcg aagctggtat tcgtccagct
     1021 ggaaatgtca tctgcgatgg aggcgtcttc acgcagttga atatcgcggc tccaggtgta
     1081 ccccaccagt ggcaggttca gcgtctggtc gagtctctcc agctccccga tgagaaaggc
     1141 accagagctg tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc
     1201 caggaaattt ctgcattgcc gtcagcatca ccggcacctg tgaattcagc gttggtcagc
     1261 accacgtttt tgccactgac tgacgtggac atgaatccac ccagaggcac tttgatggat
     1321 tcatcagtgg agatcgcctt ttttgatagt gctggcatca aaatcagaac cgagattaac
     1381 ggtcacgtag ccacgcttca tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg
     1441 aaccatgtcc ggctgcgacg tggtcggata agggcgcacg tagatcccct tcaccttgtc
     1501 tgcggtatca ccatctgcca gcggcacgaa aaaaccgtca tcatcgtatt taccagccag
     1561 gccataggca gcgaaggcgt tatcggattt aaggaccacc ggttcgacgg ttaagtcctg
     1621 cgggcgagag acagccccgg caatgccaac aggcatccgg tacagaaata cattattcat
     1681 tttttaccct ttacggtttg cccagaattc agcgttttgt ttgttcaggg aagcgatact
     1741 ggtcatgccc atgtttaggc gctgtgcatc gccggtggtg gcgcgggtgt ttcgcccttt
     1801 ggcaacctca gacacggcat taaacgccat gtcgaccgat tgtttcggta atttgcggat
     1861 atccgcatca ccgactatct ggcgaaccag cgttttgtca gcagaagcca gaacctcgcg
     1921 tttgaacgcg gtcggtttca tcttacggct cagatcgata cccggaacga taacttcggc
     1981 acgccaggct gagtcaccag taatcgtggt ttcctcttca tcgtcctcgc cgtcaccggt
     2041 cggattatcg tcaggcttat tatcgttatc gcccgtggca tttctttcca gcttagccag
     2101 cagggctttc agtaatgttt tgaggtcatc atcactgtcg ccggttggat ctccgcccat
     2161 ctctggtgct ttgtccggta gtggttgctg cggggacagg ttgatgttga gattaacgcc
     2221 ctgcggcaaa tccccctcat ctcctgtaac cgatgcggga gccgactcca ccagttcgtt
     2281 catggtgtcg gcatctcctg tcttgatggc cgcacgcatg cggttccacc agtttttctt
     2341 ttgatttgcc attgtgtctc tgtctccaat tgcacaacga tttccggctc tgcctttagg
     2401 gacaagagcc acatggtttc cggtaatatc gacctgctcg gctttacatg gctcggtctg
     2461 ctcgtactcc gcgtcatagc cgcacgacac ttcgcgcagg ccatcttcga taagctgaat
     2521 ggcgctttcg tctttgacga taaggtcagc cagcatcaaa tcagactgct cacccgtccc
     2581 gcgccggaca ttctggaggt gcccgacagc aagcactttc cagttctcgg gatttaccag
     2641 ccgcacattc ccgttttcat cttcaggatg caggatcgtg atgctcatcc cttcgaatga
     2701 ggcaagcgtg gccggatgga atacctgctc aggagaacgc gtgacgacta tttcaacgaa
     2761 cttatcgggt ttcagttttg gcaggtcatc agcaccatag agctgcttac ctgttcgtcc
     2821 tatcggcacg tctttgcaca gcaacgagcc gtcagccagg cggtatcggg tttccccgag
     2881 cctggtctca aaaaagtatt tcatgggtta tgctgccctt ttcattgcga atggaacgcg
     2941 aacaaacacg atctctttat agcagcggca gtttggcagc tcgcccgcgt ggccggtcat
     3001 gccgtccaaa gttggaggtg aatcccagcg gacgaattta cctttcatgt gatcgtggga
     3061 atctcggaca tcgccatcat cggctgtacg ccaaatataa ccatccgagc caatagctat
     3121 cgctcgggcc atatccagcg cgcccgttgc tcttcccagt tccgttcggg caatcagatc
     3181 ggccctagac ttagcaacat cgccggtaga tgcaatctct ttagcaaact gctccgcccg
     3241 accaccggtg acaacagcct ctatcgcttt gttctggatg tcgtacaccc tgtctgcggc
     3301 ctcgagaggc agagatttga tgtacttgac ctgttcggcg atgatggatt gcatcacctg
     3361 gcccacagga gcgctttcca caagattgcg gagctcgcga ctgatgttct tgctgtgttg
     3421 ccgccaaact ttctcgttct gccgggttag gtccgcagta aagttttccg cgacctttgt
     3481 cgcccagggg gtgatgattt cactgtaacg ctccagcgcc gcaataattt ccgtgatact
     3541 gtcatttgaa ccatcgtagc gaccatttac gatgtctccg accgccctcg ctatcctgcg
     3601 taggctggtt cgataccgga tttccgcctg acggctcctg cggttcgtca tcaggttcgc
     3661 cgatgccggg cggcgcttca tcttcggcat tctcgatgtc ctcgttggta atggatgccc
     3721 ctatgccggt tacgtcagaa ttttctcgca aatcagtcat agcggctttc agtgtcatca
     3781 gaccatcacc cagcgccgta ctgattgcgt tggtggtgtt taacgccacc gttgagcgat
     3841 cgacatcaga catttgccag agcgggttaa actcaaacgt gaaatcgtcc ggcagcggct
     3901 tgccaagttc cgaacgatgc atgatgtcca gtatccgccg caccggaaga cgtaaacgcc
     3961 tctcctgcaa cgaactgatg cgatcgtaat agttggcaag gtctgcatca ccggtagaaa
     4021 atcctttcgg ggactgtccg aacaaccgca ccagtgggat accaacagcg ccactaatct
     4081 gttctgcaaa ctgcgaaagg atgtcatcca gaccactgaa gctgtactga tgggtttcaa
     4141 acttatcccg cgagtccatg agcgtcatgc cttcattact ctggaactgt cgaatcaggt
     4201 cgatattctt cagcaacgct tcatacgcag gaccaccaag tgcgataagc tcgcgtagct
     4261 tctccacgct gtaggtacgc aaatgcgcct tgtagaccag ctgcgccgcg ccgacagtag
     4321 cgctgtcgaa cgcggtaaga cgatcccaga tacgctctac aaccgacatt ccccattcgt
     4381 tctcggtcat cttctgctga aatggcaacg tgacgccatc aaagcgaatc aggcgactgt
     4441 gatgaatgcg ccaggcagga attcccgttg ctgtggtcac cacatcgtaa aactcaggtt
     4501 tacccaggtc cggccccata tctttaatgc ggcgggtcag tgccgggtcg accatccagc
     4561 ggtcgagcgg gagaatcccc ttaaacttgc ccttaccgat ggtttcgggt cgcagcgggg
     4621 tcattggtgc ctgcccctca atcatgatga aacccaccgc gccgccgtag aggcgcgacc
     4681 atttcagcac gtcattcagc gcatcccaga tttgcaactc atccagttgt gattcgagaa
     4741 tgccacgatc ttttgcatca atttccgaag tgatgcgaat gcctttgcgg gtcatatcat
     4801 ccgggatagc atcgactgct tcgccgatga tccaggatga acgataggac cattccacca
     4861 gcatgcggtt acgactggtg aaattagccc ggtaggtgga tgctgagtgc tggttaggtg
     4921 tctgcatccc tactcgggca ataaaattct cataaccatc agctgtggcc tgcacagttc
     4981 gccgcagggc ttgtttgttt cgtgccatca ggcctgtctc cccagctgtt cccagatatc
     5041 cagcgatgta tcgattggcg cgaaggccat aatgaatgca tcagcaacgt ttggtgacgg
     5101 tatctcgcgt tttgcgaggt cttttttact ttcgaccatc acacgtccgt tacggtcgaa
     5161 atcacgatga ggtgttgtca gttccagttt cagcttttca agcaacggac aacgagaatc
     5221 tatgctgatc agctcatcca caggatactg ttctccgttg ttaatggcgt taaacgtatt
     5281 tctgaaacgg tcagccacca tgcctgagcc ttaagatttg cgaaaaagtc tttgttgggg
     5341 atgccgttgt actcgtcatc tggctcatgc acaccagcac cggcgttaaa cctctggtaa
     5401 ttcacacgtc gcgcgtatgc gttctcgctc ttccggtcag cgttaatttc agagaattta
     5461 gcaccggcag acgcaccaac accgatagag tcgtaaacaa tatctgcttc acgctccagc
     5521 gccgcctgat aagtacgctg gcagctcttc agtaattcat cttctttggc cttccattcg
     5581 tcggcccaga aaacaacgga tccgtgacgg taaacgttag cgcacttatc tgtaccactg
     5641 tcagccacgt caaagccaat acgctttctt ccactgggtt cgaaatttaa cgttttgtgc
     5701 gcatccactg cggcttctat ccaagacagt ttgatga
//
LOCUS       DAEEUE010000174         5702 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.174, whole genome shotgun sequence.
ACCESSION   DAEEUE010000174 DAEEUE010000000
VERSION     DAEEUE010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.174"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..219)
                     /locus_tag="KLK84_003899"
     CDS             complement(<1..219)
                     /locus_tag="KLK84_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HBE2011034.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
     gene            complement(216..1124)
                     /locus_tag="KLK84_003900"
     CDS             complement(216..1124)
                     /locus_tag="KLK84_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBE2011035.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAVFSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            1320..2087
                     /locus_tag="KLK84_003901"
                     /pseudo
     CDS             1320..2087
                     /locus_tag="KLK84_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
     gene            complement(2138..2790)
                     /gene="pphB"
                     /locus_tag="KLK84_003902"
                     /pseudo
     CDS             complement(2138..2790)
                     /gene="pphB"
                     /locus_tag="KLK84_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(2896..5457)
                     /gene="mutS"
                     /locus_tag="KLK84_003903"
     CDS             complement(2896..5457)
                     /gene="mutS"
                     /locus_tag="KLK84_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBE2011036.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            5533..>5702
                     /locus_tag="KLK84_003904"
     CDS             5533..>5702
                     /locus_tag="KLK84_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011037.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
BASE COUNT         1339 a         1480 c         1470 g         1413 t
ORIGIN      
        1 ctgttgcaac cagctcagag ccgccagcga ttgctgtgtg gcttcaacca ccggacagga
       61 gcgcgttttc aggctgatca ccagtgcgtc gattgcttcc ggcattttac ctgttggaac
      121 accgttaatt tgtaccgttg gtagaccgtt ttccaccaga aaactggcga tatccgtcgc
      181 gccggtaaaa tcatcggcga taacgccaat cttgatcatg atttcgctcc cggtagagtg
      241 atgccagaga aaatcttgat aactgcgcta tcgtcttctt tcccgtaacc cgcgttactg
      301 gcgctggtga acatattcaa tgctgttgag gccaatggca gcgggaagtg cagggctttg
      361 gctgtatcgg caaccagacc aagatcctta acaaaaatat cgacggctga atgcggggtg
      421 taatcgccat ccaccacatg acgcatccgg ttttcgaaca tccaggaatt tccggcggca
      481 ttggtcacga cgtcatacat cacatccagc gggatccccg cacgggctgc aagtgccatc
      541 gcttcggctc cggcagcaat atgtacgccc gctaacaact ggtgaataat ttttacggtc
      601 gaacctagtc ccggttctgc acctatgcga taaacttttc cggcaacggc ttccagcacg
      661 ggtgccagtc gttcaaaggc aatatcgcta ccggaggcca tgacagtcat ttcaccgtta
      721 gcggctttta ctgcaccacc agaaactggc gcatccagca tttccagatc gaatccagcc
      781 agagcggtag caatttcttg cgcatcagca ctagcgatag tggaagaaac cattactgcc
      841 gtaccgggtt tcagatgttg tgcaacgcct gtttcaccaa acagcacctg tttaacctgg
      901 gccgcattga ccaccagcac cagcagagcg tccagttttt cggcaaacgt cgcggcgtta
      961 tcagaaaccc cgcaagcacc tgcctctttc aacgtagcgc aggcattgct gttcaggtct
     1021 gcgccccagg tagaaaaaac tgcgcggaca catgacagtg ctgctcccat tcccattgac
     1081 cctaagccaa cgataccgac atgaaactca gatcccgttt tcatctgctc tccttgttaa
     1141 tttaagtgat attttgtttg atattgtgaa cataagcgct gggagataac gatatggtga
     1201 gatgattcac ataaattaac attttgtgtt attttatgtg aactaagcgt tagttgcgcc
     1261 gcgcacgttt cgcaggcaaa tagcgtagaa tgtcagcagg acaaaggaag gagcaaaagt
     1321 tgatacccgt agagcgtcgc caaatcatcc ttgagatggt agctgaaaaa ggcattgtca
     1381 gtattgctga actaacggac agaattaatg tgtcacatat gaccattcgt cgggatttac
     1441 aaaaactgga gcagcaggga gccgttgtgc tggtgtccgg aggcgtctag tctccgggac
     1501 gcgtggcgca tgaaccttct catcaggtaa aaactgcgct ggcaatgacg caaaaagcgg
     1561 ctattggcaa gctggcggca agtcttgttc tgccgggaag ttgtatctat ctggatgcgg
     1621 gaacgaccac gttagcgata gcacagcatc tgattcacat ggagtcactg actgtggtca
     1681 caaacgattt cgttattgcg aactacttgc tcgacaacag taattgcaca attattcaca
     1741 ctggcggtgc agtgtgtcgg gaaaaccgtt cctgtgtcgg ggaagccgct gcgaccatgc
     1801 tgtgcagcct gatgattgat caggctttta tttctgcatc gtcatggagt gtgcggggga
     1861 tttctacgcc agcggaagat aaagtcacgg tgaaacgggc gattgccagt gccagccgcc
     1921 agcgagtttt ggtctgtgat gcgacgaaat atggtcaggt ggcgacatgg ctggcgttac
     1981 cattaagcga gtttgatcag attattacag acgacggttt gccggagagt gccagtcgcg
     2041 cgctggcgaa gcaggatctc tctttgctgg tagcgaaaaa tgaataatgg cctgcaataa
     2101 catctggtta ctcatgcttc acagaagaag catgagacta ccttatttta taaaatgaca
     2161 gccgcccact tttcggcgat ccggtatcaa tataaatctg gttagcgaac gtctgaatgt
     2221 tatcaaacat catatgtcca aatataaaat aatcagcgcc gtttatttgt tgtaactcgc
     2281 cattaagcga tttctgcaca cgataaacag gccagagtaa ttcgctctcc gctatttctt
     2341 tgccaaagag atattcatca cccggataat ctgcatgtgc gatgacatat tttattatgt
     2401 cgttagtgat ttcaataata tgtggaaggt gatggaattt cagcaacaga tctattgcct
     2461 cttgttgcaa tcatttaaat cgaaaaacca gtcaccaccg ctggcaagcc acatattgcc
     2521 atcgccggtc gcgaacgcat ccagcgccat tgcttcgtgg ttgcctttaa cggagataaa
     2581 ccagggctgg tttagcaggc gcaggacgtc aagactctcc ggcccacgat caatattatc
     2641 gccgacagaa ataagtaagt cggtttcggg gaaaaaagag agttgatgta agcgggattg
     2701 taataactga tattcaccat gaatatcacc aacgacccat atatggcgat agtgatgggc
     2761 attgattttt tggtagcgtg tagatggcat ggttttaccc tgtaaaataa gctttcctat
     2821 tatacagggt atttttattt gattcgtcag ttgtcgttaa tattcccgat agcaaaagac
     2881 tatcgggaat tgttattaca ccagactctt caagcgataa atccactcca gcgcctgacg
     2941 cggagtcagt gaatccgggt cgagattttc cagtgcttcg accgcaggcg aagtttcttc
     3001 tggcactgac agcaaagaca tctgcgtacc atccacttgc gtcgcggcgg cgttcggcga
     3061 aatgctttcc agctcacgca gtttttgccg tgcgcgctta ataacctctt tcggtacgcc
     3121 cgccagagca gcaaccgcca gaccgtagct tttgctcgcc gcgccgtcct gcacgctgtg
     3181 cataaaggcg atagtgtcgc cgtgctccag cgcatcgaga tgtacgttgg cgacgccttc
     3241 cattttctcc ggtagctggg tcagctcgaa atagtgggtg gcaaacagcg tcaatgcctt
     3301 aatcttattc gccagatttt ctgcgcacgc ccacgccagc gacagaccat cgtaggtgga
     3361 cgttccacgc ccgatctcat ccattaacac cagactgtat tcggtggcgt tatgtaaaat
     3421 attggcggtt tcagtcatct ccaccataaa ggttgagcgc ccggaagcca ggtcatctgc
     3481 cgcgcctacg cgggtaaaga tgcgatcgat aggtccaatc tcgacttttt gtgccggtac
     3541 atagctgccg atgtaggcca tcagcgcaat cagtgcggtc tggcgcatat aggtactttt
     3601 accgcccatg ttcgggccgg taataatcaa catacggcgc tgcggcgaca gattcagcgg
     3661 gttagcgata aatggctcat tcagtacttg ttcaactacc ggatggcgac cttcggtaat
     3721 gcgaatgccc ggtttatcaa tgaaggtcgg gcaggtgtag ttcagggtat aggcccgttc
     3781 cgccaggtta accagcacgt cgagttccgc cagcgcgctc gcgctctgtt gcaacgcttc
     3841 cagatgcggc aacagcaggt cgaacagctc ttcataaagc tgtttttcca gtgccagtgc
     3901 tttgcctttt gaggtgagaa ctttatcttc gtactctttt agctctggaa tgatgtagcg
     3961 ctcggcgttt ttcagcgtct ggcgacgcat gtagttgatg ggtgccagat ggctttgccc
     4021 acggctgatt tgaatgtagt agccgtgcac cgcattaaag ccaactttta gcgtgtccag
     4081 gccggtacgt tcacgctcgc ggacttccag acgctccaga taatcggtcg cgccgtcagc
     4141 cagcgcgcgc cactcatcca gctcttcgtt atagcccgat gcgataacac caccgtcgcg
     4201 taccagcacc ggcggtgtgt cgatgattgc tcgctccagc agatcgcgca gctcggcaaa
     4261 ctcgcccatc ttctcacgta gcgcctgtac cggtgcacta tcgacagttt ctaactgcgc
     4321 acgcagctcc ggcagttgct ggaaagcgtg gcgcatacgg gccagatcgc gtgggcgagc
     4381 agttcgtaaa gccagacgtg ccagaatacg ttccaggtcg ccgacctgac gcagtaccgg
     4441 ctgcaactcg gcggtgaaat cctgcaatgc gccaatagtt tgctggcgct caagcaacac
     4501 gcgggtatcg cgcactggca tatgcagcca gcgtttcagc atacgactac ccatcggcgt
     4561 gacggtgcag tcgagcacag aagccagcgt attttccgca ccgcccgcca ggttctgagt
     4621 aatttccagg ttacgacgtg tcgcggcatc cataatgatg ctgtcctgct cacgttccat
     4681 ggttatagaa cgaatatgcg gcagggtcgt gcgttgggta tctttcgcat actgcaacag
     4741 acaaccggca gcacaaagtc cgcgcggcgc gttctcgacg ccaaaaccga ccagatcgcg
     4801 ggtgccaaat tgcagattca actgctggcg cgcggtgtcg atttcaaact cccacagcgg
     4861 gcgacggcgc aggccgcgac ggccttcaat cagcgacatc tcggcgaaat cttctgcata
     4921 cagcagttcc gccggattag agcgttgcag ctctgccgcc atcgtttcgc ggtcggccgg
     4981 ttcgctcagg cgaaaacgcc cggagctgat atccagcgtc gcgtagccga aacctttgct
     5041 gtcctgccag atagccgcca gcaggttgtc ctgacgctcc tgtaacaggg cttcatcgct
     5101 gatggtgcct ggcgtaacga tacgcacaac tttgcgctca accggacctt tgctggtcgc
     5161 cggatcgcca atttgttcgc agatggcaac ggactctccc tgattcacca gtttggcgag
     5221 atagttttcc accgcatggt agggaatccc cgccatcggg atcggctctc ccgccgaagc
     5281 accgcgtttg gtcagtgaaa tatccagcag ttgcgacgcg cgttttgcgt cgtcataaaa
     5341 cagttcataa aaatcaccca tccggtaaaa cagcaggatc tcgggatgct gggctttcag
     5401 cttgagatac tgctgcatca tgggcgtatg ggcgtcgaaa ttttctattg cactcatggg
     5461 gttatgtccg gttccctgat attaaatggg gtgtgatggt ttttattttc gttgtaatca
     5521 cataaggcgc acatgatacc ggagttaatc atttcagggg aagaagcgaa cggcgagttc
     5581 ttgatgctgg ttccgggaaa gatgctgttt tgcagctttt gcgtgatgct tatgaagcaa
     5641 gtacgcaaaa ttgtatcgat tggcgaaccc ttcttcttta gagtcatagt gccctttaag
     5701 at
//
LOCUS       DAEEUE010000175         5690 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.175, whole genome shotgun sequence.
ACCESSION   DAEEUE010000175 DAEEUE010000000
VERSION     DAEEUE010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5690
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.175"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(23..589)
                     /gene="satP"
                     /locus_tag="KLK84_003905"
     CDS             complement(23..589)
                     /gene="satP"
                     /locus_tag="KLK84_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HBE2011038.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(738..1451)
                     /gene="msyB"
                     /locus_tag="KLK84_003906"
     CDS             complement(738..1451)
                     /gene="msyB"
                     /locus_tag="KLK84_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HBE2011039.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(1477..1881)
                     /locus_tag="KLK84_003907"
     CDS             complement(1477..1881)
                     /locus_tag="KLK84_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HBE2011040.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            2258..4174
                     /gene="dnaK"
                     /locus_tag="KLK84_003908"
     CDS             2258..4174
                     /gene="dnaK"
                     /locus_tag="KLK84_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBE2011041.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4263..5393
                     /gene="dnaJ"
                     /locus_tag="KLK84_003909"
     CDS             4263..5393
                     /gene="dnaJ"
                     /locus_tag="KLK84_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBE2011042.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(5497..5649)
                     /gene="mokC"
                     /locus_tag="KLK84_003910"
     CDS             complement(5497..5649)
                     /gene="mokC"
                     /locus_tag="KLK84_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HBE2011043.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         1496 a         1410 c         1495 g         1289 t
ORIGIN      
        1 gagcgggggg atctcaagac aattagtggg attcaccaat cggcagaacg gtgcgaccaa
       61 actgctcgtt cagcacttca cccatcgcca gatagattgc gctggcaccg cagatcagac
      121 caatccagcc ggcaaagtgg atgatcgcgg cattaccggc aatgttaccg atcgccagca
      181 gggcaaacag cacggtcagg ctaaagaaaa cgaattgcag gacgcgcgcg cctttcagag
      241 tgccgaagaa cataaacagc gtaaatacgc cccacagacc caggtagaca ccaaggaact
      301 gtgcatttgg cgcatcggtc agacccagtt tcggcatcag cagaatcgca accagcgtca
      361 gccagaaaga accgtaagag gtgaatgcgg ttaaaccgaa agtgttgcct tttttgtact
      421 ccagcagacc agcaaaaatt tgcgcgatgc cgccgtagaa aatgcccatg gcaagaataa
      481 taccgtccag agcgaaataa cccacgttgt gcaggttaag cagaatggtg gtcatgccga
      541 agcccatcag gcccagcggt gccggattag ccaacttagt gttgcccata attcctcaaa
      601 aatcatcatc gaatgaatgg tgaaataatt tccctgaata actgtagtgt tttcagggcg
      661 cggcataata atcagccagt ggggcagtgt ctacgatctt ttgaggggaa aatgaaaatt
      721 ttccccggtt tccggtatca gacctgagtg gcgctaacca tccggcgcag gcaggcgatt
      781 tgcagtacgg ctggaatcgt cacgcgatag gtgctgccgc tgaccgcttt aaccccattt
      841 aatgctgcgc ccacagggcc tcccagcccc gcgccgcgca gcaaaccatg cccaagtacg
      901 ctcattgctg cgtgggtgcg taaaatgcgg gtcagttggc tggaaagcaa atgcgacaca
      961 ccttttgcca ataatttgtc tttcatcagc agcggcagca gctcttccag ctcattcacc
     1021 ctggcatcga ccgcgtgcag aaactcctgc ttatgttcct cgtccatttt cttccaggta
     1081 ttacgcagaa attgttccag taactgttgc tcaatttcaa acgtagacat ctctttgtcg
     1141 gctttcagct tcaatcgctt tgaaacatcg agcaaaatgg cccgatacaa tttaccgtgt
     1201 ccgcgcagtt tgttggcgat actatcgcca ccaaaatgct gtaattctcc ggcaatcagc
     1261 tgccagttgc ggcgatgttg ctcgggatgc ccttccatcg atttaaacag ttcgttgcgc
     1321 atcagtatgc tggagaggcg agttttgcct ttttcattat gggtgagcaa tcgggcgaaa
     1381 tttgccaact gttcctcact acaatgctga agaaaatcca gatctgaatc attcaggtaa
     1441 ttaacattca ttttttgtgg cttctatatt ctggcgttag tcgtcgccga taattttcag
     1501 cgtggccata tccgatgagt tcaccgtatg accggaaaag gtgatttttg agacgcagcg
     1561 tttattgtcg ttatcgctgt taatgttgat ccagtcagtg gtttgccctt cttttatttc
     1621 tgaaggaata ttcaggctct gactggcgct acgggcggct ttgaaataaa ccgatgcacc
     1681 acttaactgt aaatcgccat ggtcggcaga gagttgtatg cgtttcacaa tgcgacaaac
     1741 aggaagtttc agcgccagat cgttggtttc gttacgcggc attgcaatga cgccgaggag
     1801 tttatggtcg tttgcctgcg ccgtgcagca cagcatcagg ctaatcgcca ggctggcgga
     1861 aatcgtaaaa acggatttca taaggattct cttagtggga agaggtaggg ggatgaatac
     1921 ccactagttt actgctgata aagagaagat tcaggcacgt aatcttttct ttttattaca
     1981 attttttgat gaacgccttg gctgcgattc attctttata tgaataaaat tgctgtcaat
     2041 tttacgtctt gtcctgccat atcgcgaaat ttctgcgcaa aagcacaaaa aatttttgca
     2101 tctccccctt gatgacgtgg gttacgaccc catttagtag tcaaccgcag tgagtgagtc
     2161 tgcaaaaaaa tgaaattggg cagttgaaac cagacgtttc gcccctatta cagactcaca
     2221 accacatgat gaccgaatat atagtggaga cgtttagatg ggtaaaataa ttggtatcga
     2281 cctgggtact accaactctt gtgtagcgat tatggatggc accattcctc gcgtgctgga
     2341 gaacgccgaa ggcgatcgca ccacgccttc tatcattgcc tatacccagg atggtgaaac
     2401 tctggttggt cagccggcta aacgtcaggc agtgacgaac ccgcaaaaca ccctgtttgc
     2461 gattaaacgc ctgattggcc gccgcttcca ggacgaagaa gtacagcgtg atgtttccat
     2521 catgccgttc aaaattattg ctgctgataa cggcgacgca tgggtcgaag ttaaaggcca
     2581 gaaaatggca ccgccgcaga tttctgctga agtgctgaaa aaaatgaaga aaaccgctga
     2641 agattacctg ggtgaaccgg taactgaagc tgttatcacc gtaccggcat actttaacga
     2701 tgctcagcgt caggcaacca aagacgcagg ccgtatcgct ggtctggaag taaaacgtat
     2761 catcaacgaa ccgaccgcag ctgcgttggc ttacggtctg gacaaaggca ctggcaaccg
     2821 tactatcgcg gtttatgacc tggggggtgg tactttcgat atttctatta tcgaaatcga
     2881 cgaagttgac ggcgaaaaaa ccttcgaagt tctggcaacc aacggtgata cccacctggg
     2941 tggtgaagac ttcgacagcc gtctgatcaa ctatctggtt gaagaattca agaaagatca
     3001 gggcattgac ctgcgcaacg atccgctggc aatgcagcgc ctgaaagaag cggcagaaaa
     3061 agcgaaaatc gaactgtctt ccgctcagca gaccgacgtt aacctgccat acatcactgc
     3121 agacgcgacc ggtccgaaac acatgaacat caaagtgact cgtgcgaaac tggaaagcct
     3181 ggttgaagat ctggtaaacc gttccattga gccgctgaaa gttgcactgc aggatgctgg
     3241 cctgtccgta tctgatatcg acgacgttat cctcgttggt ggtcagactc gtatgccaat
     3301 ggttcagaag aaagttgctg agttctttgg taaagagccg cgtaaagacg ttaacccgga
     3361 cgaagctgta gcaatcggtg ctgctgttca gggtggtgtt ctgactggcg acgtaaaaga
     3421 cgtactgctg ctggacgtta ccccgctgtc tctgggtatc gaaaccatgg gcggtgtgat
     3481 gacgacgctg atcgcgaaaa acaccactat cccgaccaag cacagccagg tgttctctac
     3541 cgctgaagac aaccagtctg cggtaaccat ccatgtgctg cagggtgaac gtaaacgtgc
     3601 ggctgataac aaatctctgg gtcagttcaa cctagatggt atcaacccgg caccgcgcgg
     3661 catgccgcag atcgaagtta ccttcgatat cgatgctgac ggtatcctgc acgtttccgc
     3721 gaaagataaa aacagcggta aagagcagaa gatcaccatc aaggcttctt ctggtctgaa
     3781 cgaagatgaa atccagaaaa tggtacgcga cgcagaagct aacgccgaag ctgaccgtaa
     3841 gtttgaagag ctggtacaga ctcgcaacca gggcgaccat ctgctgcaca gcacccgtaa
     3901 gcaggttgaa gaagcaggcg acaaactgcc ggctgacgac aaaactgcta tcgagtctgc
     3961 gctgactgca ctggaaactg ctctgaaagg tgaagacaaa gccgctatcg aagcgaaaat
     4021 gcaggaactg gcacaggttt cccagaaact gatggaaatc gcccagcagc aacatgccca
     4081 gcagcagact gccggtgctg atgcttctgc aaacaacgcg aaagatgacg atgttgtcga
     4141 cgctgaattt gaagaagtca aagacaaaaa ataatcgccc tataaacggg taattatact
     4201 gacacgggcg aaggggaatt tcctctccgc ccgtgcattc atctaggggc aatttaaaaa
     4261 agatggctaa gcaagattat tacgagattt taggcgtttc caaaacagcg gaagagcgtg
     4321 aaatcagaaa ggcctacaaa cgcctggcca tgaaatacca cccggaccgt aaccagggtg
     4381 acaaagaggc cgaggcgaaa tttaaagaga tcaaggaagc ttatgaagtt ctgaccgact
     4441 cgcaaaaacg tgcggcatac gatcagtatg gtcatgctgc gtttgagcaa ggaggcatgg
     4501 gcggcggtgg ttttggcggc ggcgcagact tcagcgatat ttttggtgac gttttcggcg
     4561 atatttttgg cggcggacgt ggtcgtcaac gtgcggcgcg cggtgctgat ttacgctata
     4621 acatggagct caccctcgaa gaagctgtac gtggcgtgac caaagagatc cgcattccga
     4681 ctctggaaga gtgtgacgtt tgccacggta gcggtgcaaa accaggtaca cagccgcaga
     4741 cctgtccgac ctgtcatggt tctggtcagg tgcagatgcg ccagggattc ttcgctgtac
     4801 agcagacctg tccacactgt cagggccgcg gtacgctgat caaagatccg tgcaacaaat
     4861 gtcatggtca tggtcgtgtt gagcgcagca aaacgctgtc cgttaaaatc ccggcagggg
     4921 tggacactgg agaccgcatc cgtcttgcgg gcgaaggtga agcgggcgag catggcgcac
     4981 cggcaggcga tctgtacgtt caggttcagg ttaaacagca cccgattttc gagcgtgaag
     5041 gcaacaacct gtattgcgaa gtcccgatca acttcgctat ggcggcgctg ggtggcgaaa
     5101 tcgaagtacc gacccttgat ggtcgcgtca aactgaaagt gcctggcgaa acccagaccg
     5161 gtaagctatt ccgtatgcgc ggtaaaggcg tcaagtctgt ccgcggtggc gcacagggtg
     5221 atttgctgtg ccgcgttgtc gtcgaaacac cggtaggcct gaacgaaaag cagaaacagc
     5281 tgctgcaaga gctgcaagaa agcttcggtg gcccaaccgg cgagcacaac agcccgcgct
     5341 caaagagctt ctttgatggt gtgaagaagt tttttgacga cctgacccgc taacctcccc
     5401 aaaagcctgc ccgtgggcag gcctgggtaa aaatagggtg cgttgaagat atgcgagcac
     5461 ctgtaaagtg gcggggatca ctccccgccg ttgctcttac tcggattcgt aagccgtgaa
     5521 aacagcaacc tccgtctggc cagttcggat gtgaacctca cagaggtctt ttctcgttac
     5581 cagcgccgcc actacggcgg tgatacagat gacgatcagg gcgacaatca tcgccttatg
     5641 ctgcttcatt gctctcttct ccttgacctt acggtcagta agaggcactc
//
LOCUS       DAEEUE010000176         5682 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.176, whole genome shotgun sequence.
ACCESSION   DAEEUE010000176 DAEEUE010000000
VERSION     DAEEUE010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5682
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.176"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            872..3157
                     /gene="nrdA"
                     /locus_tag="KLK84_003911"
     CDS             872..3157
                     /gene="nrdA"
                     /locus_tag="KLK84_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HBE2011044.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3391..4521
                     /gene="nrdB"
                     /locus_tag="KLK84_003912"
     CDS             3391..4521
                     /gene="nrdB"
                     /locus_tag="KLK84_003912"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HBE2011045.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4521..4775
                     /gene="yfaE"
                     /locus_tag="KLK84_003913"
     CDS             4521..4775
                     /gene="yfaE"
                     /locus_tag="KLK84_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HBE2011046.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4829..5479)
                     /gene="inaA"
                     /locus_tag="KLK84_003914"
     CDS             complement(4829..5479)
                     /gene="inaA"
                     /locus_tag="KLK84_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HBE2011047.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
BASE COUNT         1456 a         1487 c         1349 g         1390 t
ORIGIN      
        1 atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca actcgctttg
       61 atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga gaaaagagat
      121 gcaatcattg acgggagtag agataaatac tccttgcata gaaagataat ccgcatatta
      181 atctgaccat cattttatat aagacggcat atttaaacgc attatacaat atgggttatc
      241 tttcttttgt aaattgtcca acccgatgca atggctattt tgttattatt tgatgaataa
      301 tatcagtgcg tcataattca agttaataac cttaagggat accagttata tttaaactaa
      361 attaaagtaa tgaataattt tcttataata taaggtaaat taacaaaatg gcttcgcatt
      421 taacaataac cgaatagaaa acaaccattt cgacatcaac aatcgcttac attcgcttat
      481 atattgacca caactgatac atcagattat gtgatgactc gtgcttagat caatttttgc
      541 aatcattagc aaaaagatta ataaaccatc tatatcaatt tatccaacct attctgccgt
      601 tcaagaaatc gccgaacagt tatttttaac aaatttttct cttcccattg actttcccgg
      661 acaccttgtc tgacctaagg tgcgcgaaag ccactttttc cttcctgagt tatccacaaa
      721 gttatgcact tgcaagaggg tcattttcac actatcttgc agtgaatccc aaacataccc
      781 cctatatata gtgttctaag cagcttccct tactacaggt agtctgcatg aaactattgc
      841 ggaaagaatt ccaaaaacag gtacgacata catgaatcag aatctgctgg tgacaaagcg
      901 cgacggtagc acagagcgca tcaatctcga caaaatccat cgcgttctgg attgggcggc
      961 agaaggactg cataacgttt cgatttccca ggtcgagctg cgctcccaca ttcagtttta
     1021 tgacggtatc aagacctctg acatccacga aactattatc aaggctgccg cagacctgat
     1081 ctcccgtgat gcgccggatt atcagtatct cgccgcgcgc ctggcgatct tccacctgcg
     1141 taaaaaagcc tacggccagt ttgagccgcc tgcgctgtac gaccacgtgg tgaaaatggt
     1201 cgagatgggc aaatacgata atcatctgct ggaagactac acggaagaag agttcaagca
     1261 gatggacacg tttatcgatc acgaccgtga tatgaccttc tcttatgctg ccgttaagca
     1321 gctggaaggc aaatatctag tacagaaccg cgtgaccggc gaaatctacg aaagcgccca
     1381 gttcctttat attcttgttg ccgcgtgcct gttctcgaac tacccgcgtg aaacgcgcct
     1441 gcaatacgtg aagcgttttt atgacgcggt ttccacattt aaaatttcgc tgccgacgcc
     1501 aatcatgtcc ggcgtgcgta ccccgactcg tcagttcagc tcctgcgtac tgatcgagtg
     1561 cggtgacagc ctggattcca tcaacgccac ctccagcgcg attgtgaaat atgtttccca
     1621 gcgtgccggg atcggcatca acgccggacg tattcgtgcg ctgggcagcc cgattcgcgg
     1681 tggcgaagcg ttccataccg gctgtatccc gttctacaaa catttccaga cagcggtgaa
     1741 atcctgctct cagggcggtg tgcgcggcgg tgcggcaacg ctgttctacc cgatgtggca
     1801 tctggaagtg gaaagcctgc tggtgttgaa aaacaaccgt ggggttgaag gcaaccgcgt
     1861 gcgtcatatg gactacgggg tacaaatcaa caaactgatg tatacccgcc tgctgaaagg
     1921 tgaagatatc accctgttca gcccgtccga cgtaccgggg ctgtacgacg cgttcttcgc
     1981 cgatcaggaa gagtttgaac gcctgtatac caaatatgag aaagacgaca gcatccgcaa
     2041 gcagcgtgtg aaagccgttg agctgttctc gctgatgatg caggaacgtg cgtctaccgg
     2101 tcgtatctat attcagaacg ttgaccactg taatacccat agtccgtttg atccgaccat
     2161 cgcgccagtt cgccagtcta acctgtgcct ggaaattgcc ctgccgacca aaccgctgaa
     2221 cgacgtcaac gacgagaacg gtgaaatcgc gctgtgtacg ctgtctgctt tcaacctggg
     2281 cgcaattaat agcctggatg aactggaaga gctggcgatt ctggcggttc gtgcacttga
     2341 cgcgctactg gattatcagg attacccgat cccggccgcc aaacgtggag cgatggggcg
     2401 tcgtacgctg ggtattggtg tgattaactt cgcttactat ctggcgaagc acggtaaacg
     2461 ctactccgac ggcagcgcca acaacctgac gcataaaacc ttcgaagcga ttcagtatta
     2521 cctgctgaaa gcgtctaatg agctggctaa agagcaaggc gcgtgcccgt ggtttaacga
     2581 aaccacttac gcgaaaggga tcctgccgat cgatacctat aagaaagatc tggatgccat
     2641 cgctaatgag ccgctgcatt acgactggga agcattgcgt gaatcaatca aaacgcacgg
     2701 cctgcgtaac tccacgcttt ctgccctgat gccgtccgag acctcttcgc agatctctaa
     2761 cgccactaac ggtattgaac cgccgcgcgg ttacgtcagc atcaaagcgt cgaaagacgg
     2821 tattttgcgc caggtggtgc cggactacga gcacctgcac gacgcctatg agctgctgtg
     2881 ggaaatgccg ggtaacgatg gctacctgca actggtaggt atcatgcaga aatttatcga
     2941 tcagtcgatc tctgctaaca ccaactacga tccgtcacgt ttcccgtcag gaaaagtgcc
     3001 gatgcagcag ttgctgaaag acctgctcac cgcctacaaa ttcggggtca aaacgctgta
     3061 ttatcagaac acccgtgacg gcgctgaaga cgcgcaggac gatctggtac cgtcaatcca
     3121 ggacgatggc tgcgaaagcg gcgcatgtaa gatctgatat tgagatgccg gatgcggcgt
     3181 aaacgcctta tccggcctac ggctcggttt gtaggcctga taagacgcgc cagcgtcgca
     3241 tcaggctccg ggtgccggat gcagcgtgaa cgccttatcc ggcctacggc tcggatttgt
     3301 aggcctgata agacgcgcca gcgtcgcatc aggcacagga tgcggcgtaa aatgccttat
     3361 ccggcattaa actcccaaca ggacacactc atggcatata ccaccttttc acagacgaaa
     3421 aatgatcagc tcaaagaacc gatgttcttt ggtcagccgg tcaacgtggc tcgctacgat
     3481 cagcaaaaat atgacatctt cgaaaagctg atcgaaaagc agctctcttt cttctggcgt
     3541 ccggaagaag ttgacgtctc ccgcgaccgt atagattacc aggcgctgcc ggagcacgaa
     3601 aaacacatct ttatcagcaa cctgaaatat cagacgctgc tggattccat tcagggtcgt
     3661 agcccgaacg tggcgctatt gccgcttatt tctattccgg aactggaaac ctgggtcgaa
     3721 acctgggcgt tctcagaaac gattcattcc cgttcctata ctcatatcat tcgtaatatc
     3781 gttaacgatc cgtctgttgt gtttgacgat atcgtcacca acgagcagat ccagaaacgt
     3841 gcggaaggga tctccagcta ttacgatgaa ctgatcgaaa tgaccagcta ctggcacctt
     3901 ctgggcgaag gcacccacac cgttaacggt aaaaccgtaa ccgttagcct gcgcgagctg
     3961 aagaaaaaac tgtatctctg cctgatgagc gttaacgcgc tggaagcgat ccgcttctac
     4021 gtcagctttg cttgttcctt tgcctttgca gaacgcgaac tgatggaagg taacgccaag
     4081 attattcgcc tgattgcccg cgatgaagcc ctgcacctga ccggtactca gcatatgctg
     4141 aatctgctgc gcagcggcgc ggatgatccg gagatggcgg aaattgccga agaatgtaag
     4201 caggagtgct atgacctgtt tgtccaggcg gcacagcaag agaaagactg ggcggattat
     4261 ctgttccgtg acggttcgat gattggtctg aataaagata ttctctgcca gtacgttgaa
     4321 tacatcacca atatccgcat gcaggcagtc ggtttggatc tgccgttcca gacgcgctcc
     4381 aacccgatcc cgtggatcaa cacctggctg gtgtctgata acgtgcaggt tgctccgcag
     4441 gaagtggaag tcagttctta tctggtcggg cagattgacg cagaagtgga caccgacgat
     4501 ttgagtaact tccagctctg atggcccgcg ttaccctgcg catcactggc acacaactgc
     4561 tgtgccagga tgaacaccct tcccttctgg cggcgctgga atcccacaat gtggcggttg
     4621 agtaccagtg ccgcgaaggt tactgcggct cctgtcgcac ccgactggtc gcaggtcagg
     4681 tggactggat tgccgaaccg ttagccttta ttcagccggg ggaaattttg ccctgttgtt
     4741 gccgggcaaa aggcgatatt gaaatcgagt tgtgatcggg tactagcgcc agatacgacg
     4801 cttacgcgta ttatctggcc tacaacgatt acatggcgta gtaatacgct ttcacctgct
     4861 cccagtccgc tttagggatc ggctccagat atttttccag ctggcggaag tcatgattaa
     4921 tcgctttatc gcgacgcaag cggcgacgac ttttctccag atcaagaaaa ccagcttctg
     4981 ccttaccttc tgttttcaca tagatatggc gaacatagca acagccatgc tgacgattaa
     5041 cgctatgcat tttcttaaac gccagtgcca ctgctttcaa catggcttgc cgtacttcgt
     5101 cagaataagg cgatactgca tgccgggcat accagtcagc aatgctgatg aaccccgcca
     5161 tatcttcagt caccagcaac gcgcgccatt caccctcaat tttcaccgct tcaccaaaaa
     5221 cgatcttcgg cacgatgaca ccggcccgtt ccagttcttt aattaccgca acctcacgga
     5281 caatcgttgg tcggccgaac ggataacgta ctgaatgaaa cagatgatgc gtcatgcgct
     5341 ttacatacag ctttttgccg ttgcgctcga cgcattgcac cccgctcata ccattacggc
     5401 gatagttagg ctcttcaacc cagtcgccct ctgttgccca ccagtgatta aattcgtcgt
     5461 actttgctga aactgccata ccctatcgcc tgtcattttt attaacgaca atgactatag
     5521 gtggttacct gaggaaaatc ttaatgaaac gtgtcgtatt aatgaatttc ttttgccgct
     5581 acaggtgtga caccgctttt gcccttcatt tgcagcgcat agattccacc agccactacc
     5641 actaatagtg cgcccagcag ataaaatgtg aatttaatca gt
//
LOCUS       DAEEUE010000177         5642 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.177, whole genome shotgun sequence.
ACCESSION   DAEEUE010000177 DAEEUE010000000
VERSION     DAEEUE010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5642
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.177"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            263..1189
                     /gene="tauA"
                     /locus_tag="KLK84_003915"
     CDS             263..1189
                     /gene="tauA"
                     /locus_tag="KLK84_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBE2011048.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            1202..1969
                     /gene="tauB"
                     /locus_tag="KLK84_003916"
     CDS             1202..1969
                     /gene="tauB"
                     /locus_tag="KLK84_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HBE2011049.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1966..2793
                     /gene="tauC"
                     /locus_tag="KLK84_003917"
     CDS             1966..2793
                     /gene="tauC"
                     /locus_tag="KLK84_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HBE2011050.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2790..3641
                     /gene="tauD"
                     /locus_tag="KLK84_003918"
                     /pseudo
     CDS             2790..3641
                     /gene="tauD"
                     /locus_tag="KLK84_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
     gene            complement(3747..4721)
                     /gene="hemB"
                     /locus_tag="KLK84_003919"
     CDS             complement(3747..4721)
                     /gene="hemB"
                     /locus_tag="KLK84_003919"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBE2011051.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            5245..5613
                     /locus_tag="KLK84_003920"
                     /pseudo
     CDS             5245..5613
                     /locus_tag="KLK84_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1325 a         1409 c         1529 g         1379 t
ORIGIN      
        1 attgacctgc ctttagggtg cctttaaaat tcgtttttaa aggctacgat gacatttttg
       61 aagagtctta aaagaattaa aatacacggc gcgcctatct tgaatgtttt atcgatatcg
      121 aagcattgag catttttaac tcgttttatc ttgaatgcaa tcggcgcata acttattgaa
      181 tcccatcaaa tcccgtcttt ttttacccat tcccggttat cttgaataaa ttacctagtc
      241 atataacaca cttcttgccg cactggcatt catcgctttt caggcacagg cggtgaacgt
      301 caccgtggcg tatcaaacct cagccgaacc ggcgaaagtg gctcaggccg acaacacctt
      361 tgctaaagag agcggggcga gcgtggactg gcgtaagttt gacagcggag ccagcatcgt
      421 gcgggcgctg gcttctggcg acgtgcaaat cggcaacctc ggttccagcc cattagcggt
      481 tgcagccagc caacaggtgc cgattgaagt cttcttgctg gcgtcaaaac tgggtaactc
      541 cgaagcgctg gtggtaaaga aaactatcag caaacccgaa gatttgattg gtaaacgcat
      601 tgccgtaccg tttatctcca ccacccacta cagcctgctg gcggcgctga aacactgggg
      661 gattaaacca gggctagtgg agattgtgaa cctgcagccg cccgcgatta tcgccgcatg
      721 gcagcggggt gatattgatg gtgcttatgt ctgggcaccg gcggttaacg ccctggaaaa
      781 agacggcaag gtgctgaccg attctgaaca ggtcgggcag tggggtgcgc caacgctgga
      841 cgtctgggtg gtgcgcaaag attttgccga gaaacatcct gaggtcgtga aagcgttcgc
      901 caaaagcgcc atcgatgcac agcaaccgta cattgctaac cctgacgcgt ggctgaaaca
      961 gccggaaaac atcagcaaac tggcgcgttt aagcggcgtg cctgaaggtg acgttccggg
     1021 gctggtgaag ggaaatacct atctgacgcc gcagcaacaa acggcagaac tgaccggacc
     1081 ggtgaacaaa gcaatcatcg acaccgcgca gtttttgaaa gagcagggca aggtcccggc
     1141 tgtagcgaat gattacagcc agtacgttac ctcgcgcttc gtgcaataaa aggaggcgca
     1201 gatgctgcaa atctctcatc tctatgccga ttatggcggc aaaccggcac tggaagatat
     1261 caacctgacg ctggaaagcg gcgagctact ggtggtgctg gggccgtccg gctgcggtaa
     1321 aaccaccctg ctgaatctga ttgccggttt tgtgccttat cagcatggca gcattcaact
     1381 ggcgggtaag cgtattgagg gaccgggagc agagcgtggc gtagtttttc agaatgaagg
     1441 gctgctaccg tggcgcaatg tacaggacaa cgtggcgttc ggcctgcaat tggcaggtat
     1501 agagaaaatg cagcgactgg aaatcgcgca ccagatgctg aaaaaagtgg ggctggaagg
     1561 cgcagaaaaa cgctatatct ggcagctttc cgatggtcaa cgtcagcggg tggggattgc
     1621 ccgcgcgctg gcggcgaatc cccagctgtt attactcgac gaaccgtttg gtgcgctgga
     1681 cgccttcacc cgcgaccaga tgcaaactct gctgctgaaa ctctggcagg agacgggcaa
     1741 gcaggtgctg ttgattaccc acgatataga agaagcggtg tttatggcga ctgaactggt
     1801 tctgctttca tccggccctg gccgtgtgct ggagcggctg cggctcaact ttgctcgccg
     1861 ctttgttgcg ggagagtcga gccgcagcat caagtccgat ccacaattca tcgccatgcg
     1921 cgaatatgtt ttaagccgcg tatttgagca acgggaggcg ttctcatgag tgtgctcatt
     1981 aatgaaaaac tgcattcgcg gcggctgaaa tggcgctggc cgctctcgcg tcaggtgacc
     2041 ttaagcattg gcacgttagc ggttttactc actgtatggt ggacggtggc ggcgctgcaa
     2101 ctgattagcc cgctgttttt gccgccgccg caacaagtac tggcaaaact gctcaccatt
     2161 gccggaccgc agggatttat ggacgccacg ctgtggcagc atctggcagc cagcctgacg
     2221 cgcattgtgc tggcgctact cgcggcggtg gtgatcggta ttccggtcgg gatcgccatg
     2281 gggctaagcc cgacggtgcg cggcattctg gatccggtaa tcgaacttta tcgtccggtg
     2341 ccaccgctgg cttatttgcc gctgatggtg atctggtttg gtattggtga aacctcgaag
     2401 atcttactga tctatttagc gatttttgcg ccggtggcga tgtcggcgct ggcaggggtg
     2461 aaaagcgcgc agcaggttcg cattcgtgcc gcccagtcgc tgggtgccag ccgtgcgcag
     2521 gtgctgtggt ttgtcatttt gcccggcgcg ctgccggaaa tcctcaccgg attacgcatt
     2581 ggtctgggtg tgggctggtc tacgctggta gcggcggagc tgattgccgc gacacgcggt
     2641 ttaggattta tggttcagtc agcgggtgaa tttctcgcga ctgacgtggt actggcgggg
     2701 atcgcggtga ttgcgattat cgcctttctt ttagaactgg gtctgcgcgc gttacagcgc
     2761 cgtctgacgc cctggcatgg agaagtacaa tgagtgaacg tctgagcatt accccgctgg
     2821 ggccgtatat cggcgcacaa atttcgggtg ccgacctgac gcgcccgtta agcgataatc
     2881 agtttgaaca gctttaccat gcggtgctgc gccatcaggt ggtatttctg cgcgatcaag
     2941 ctattacgcc gcagcagcaa cgcgcgctgg cccagcgttt tggcgaactg catattcacc
     3001 ctgtttaccc tcatgccgaa ggggttgacg agatcatcgt gctggatacc cataacgata
     3061 atccgccgga taacgacaac tggcataccg atgtgacatt tattgaaacg ccacccgcag
     3121 gggcgattct ggcagctaaa gagttacctt cgaccggcgg tgatacgctc tggaccagcg
     3181 gtattgcggc ctatgaggcg ctctctgttc ccttccgcca gctgctgagc tggctgcgtg
     3241 cggagcatga tttccgtaaa tcattcccgg aatacaaata ccgcaaaacc gaggaggaac
     3301 atcaacgctg gcgcgaggcg gtcgcgaaaa atcctccgtt gctacatccg gtggtgcgaa
     3361 cgcatccggt gagcggtaaa caggcgctgt ttgtgaatga aggctttact acgtgaattg
     3421 tcgatgtcag cgagaaagag agcgaagcct tgttaggctt tttattcgcc catatcacta
     3481 aaccggagtt tcaggtgcgt tggcgctggc aaccgaatga tattgcgatt tgggataacc
     3541 gcgtgactca gcactatgcc aatgccgatt acctgccaca gcggcggatt atgcatcggg
     3601 cgacgatcct tggggataaa ccgttttatc gggcggggtg atacgagtgc ggacggttcc
     3661 ctcgccccct tggggagagg gttagggtga gggggcgttc actgtacttt caacaggtta
     3721 actccccctt tctgagagga aaaaaattaa cgcagaatct tcttctcagc caaatccagc
     3781 gcaaagtagc tgaaaatcag atccgcaccc gcacgcttaa ttgaacctaa gctttcgagc
     3841 acgactttct cttcatctat agcacccgcc agcgcggcga acttaatcat cgcgtactca
     3901 ccgctcacct gatacgcgcc aatcggcaat tcagtacgtt cacgcagctc acgcacgatg
     3961 tcgaggtacg ctccagcagg tttaaccatc aggcagtctg cgccctgggc ttcatccagc
     4021 agtgactcac gaatcgcctc gcgacggttc attgggttca tctgatagct tttgcggtcg
     4081 ccttttaatg cgcttcctgc agcttcacgg aacgggccat aaaaggaaga ggcgaacttg
     4141 gtcgaatacg acataatcgc cgtatcttta aagcctgcag cgtccagcgc ctgacgaatc
     4201 gcctgtacct ggccgtccat cgcggcggaa ggggcgatga agtctgcgcc tgcagcagct
     4261 gcaaccacgg cttgcttgcc taaattttcc agagtcgcgt cgttgtcgac gccatgctcg
     4321 cacagcacac cgcagtgacc gtgagaagtg tattcacaga aacaagtgtc tgacatgacg
     4381 atcatttctg gcacagtctg cttgcagatg cgcgacatac gcgccaccag tccatcttcc
     4441 cgccaggcgt cgctgccggt ttcatcggta tggtgagaga tgccaaaagt cattacggaa
     4501 cggataccgg cgttggcgat gcgttcaatt tcgcgtgcca gatgtttctc tggaatgcgc
     4561 atcacgcctg gcatggcttc aacggctttg tagtcgtcaa tttcttcttc aacaaagatc
     4621 ggcaacacca ggtcgttaag gctaagtgtt gtctcttcaa acatagcgcg cagcgcagga
     4681 gatttgcgca ggcgacgagg gcgttggatt aagtctgtca tggtctgcct gatgtttgtg
     4741 gaattaaggt gcacagtata cctgaagcgg ggcaggagtg ttttacgaaa gttgtcgcga
     4801 tgttgacaag aagagaatga aagagagacc aggacatctt tgtaatagaa atagcaataa
     4861 tttcatattc actgaaaata tttttaatct ttatttatag ctcttggtta ttattttttg
     4921 gcgtttggtt gtgctgctat aaattgaata attcaaactt tgttgcatat tgataagata
     4981 tttatctggt ttgtgttttt gatatttata acgcgtacgc atcaaaaata acattatctg
     5041 caacatactg aaatatcgtc cttttatcaa tttgatgcgt gtttttctcc ataaattata
     5101 tatgtccaca ttcggactta ggggaaagaa taattgaacc attcgtctgt aacgcagcat
     5161 aatcgttagc gcgaaacata atatgttttc tatgcattga taattgatgg atcaacttat
     5221 tacgtccctg aggagggatg acaaatgcac tcctggaaaa agaaacttgt agtatcacaa
     5281 ttagcattgg cttgcactct ggctatcacc tctcaggcta atgcagcgac caacgatatt
     5341 tctggtcaaa cttacaatac tttccatcac tacaacgacg ccacctatgc tgatgacgtt
     5401 tactatgatg gttatgtagg ctggaacaac tatgccgctg atagctatta caacggcgat
     5461 atctacccgg tcattaataa cgctaccgtt aacggcgtga tttctaccta ctatctggac
     5521 gacggtattt ctaccaatac caacgccaat agtctgacaa tcaaaaacag cactattcac
     5581 ggtatgattt attccgagtg catgacaact gattagactg gccccctgaa tctccagaca
     5641 ac
//
LOCUS       DAEEUE010000178         5580 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.178, whole genome shotgun sequence.
ACCESSION   DAEEUE010000178 DAEEUE010000000
VERSION     DAEEUE010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5580
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.178"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(1..279)
                     /locus_tag="KLK84_003921"
     CDS             complement(1..279)
                     /locus_tag="KLK84_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011052.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            220..405
                     /locus_tag="KLK84_003922"
     CDS             220..405
                     /locus_tag="KLK84_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011053.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(447..602)
                     /gene="hokD"
                     /locus_tag="KLK84_003923"
     CDS             complement(447..602)
                     /gene="hokD"
                     /locus_tag="KLK84_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBE2011054.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1254..1670)
                     /locus_tag="KLK84_003924"
     CDS             complement(1254..1670)
                     /locus_tag="KLK84_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011055.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1697..1837
                     /locus_tag="KLK84_003925"
                     /pseudo
     CDS             1697..1837
                     /locus_tag="KLK84_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1837..2877
                     /locus_tag="KLK84_003926"
     CDS             1837..2877
                     /locus_tag="KLK84_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE2011056.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSKCDFCH"
     gene            complement(2905..2994)
                     /locus_tag="KLK84_003927"
     tRNA            complement(2905..2994)
                     /locus_tag="KLK84_003927"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2958..2960),aa:Ser,seq:cga)
     gene            3088..3885
                     /gene="mtfA"
                     /locus_tag="KLK84_003928"
     CDS             3088..3885
                     /gene="mtfA"
                     /locus_tag="KLK84_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HBE2011057.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYVASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3986..4061
                     /locus_tag="KLK84_003929"
     tRNA            3986..4061
                     /locus_tag="KLK84_003929"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4019..4021,aa:Asn,seq:gtt)
     gene            4223..5485
                     /locus_tag="KLK84_003930"
                     /pseudo
     CDS             4223..5485
                     /locus_tag="KLK84_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1504 a         1322 c         1292 g         1462 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt ttgctgcttc
      601 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      661 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt
      721 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgcagtcat
      781 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      841 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      901 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
      961 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1021 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttaaacac
     1081 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1141 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1201 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1261 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1321 tcgcgtttta attgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1381 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1441 tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc cataacacct
     1501 tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt
     1561 ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct
     1621 atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat tttcattacc
     1681 gccctggtaa tgctgccacc agcgaaaatg gttaatggaa aacgggcttc cgttctatac
     1741 caatcgtagt ggcaaaccga ttgtcagccg ggatctattt acctgcaata aaactttacc
     1801 accacgcgag gtagagccga attttggtgc gatctgatgg gaagacgaag gaaaaatcct
     1861 gaacacgaaa aattacctcc aaatgtatac ccaaataaat atagttatgt atggaaacca
     1921 acatccagag aatctgtaac actaaccgcc atcaaggatg gtttagctgc tttatggaaa
     1981 aagtatgagg aaactgtaaa taatcgcgat cgtgcaatga cattcggtcg cttgtgggaa
     2041 aaattcctcg ccagcgccta ttacagtgac ctcagtccaa gaacacaaaa agattatctg
     2101 caacatcaaa aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca
     2161 gaacacatcc gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat
     2221 gaaaaaagca gtatgtcccg cgtttacagt tgggggtatg agcgagggta cgtgaaggct
     2281 aacccatgtg caggtgtaag taaattcaag gccaaaaacc gcgaacgata tgtaaccgac
     2341 aaagaatacc aggcagtatt aagcgttgca cctcttcctg tttttatcgc aatggaaatt
     2401 gcctatctgt gtgcagcgag ggtttccgat gtgttatcgc tgaaatggga gcagattgga
     2461 aacgacggga tctttatcca gcaagggaaa acaggaaaaa aacagataaa agcatggagt
     2521 ccacgattac aggcggcgat cgaaaaagca aaacagttac caacatccgc ctatgtaatc
     2581 agcaatcaat acggcaaccg atatatgtac aaaggcttta acgaaatgtg ggtagaagca
     2641 agaaatcgcg caggcaaaat ttcaggtatt ttaaccgact tcacctttca tgatctgaag
     2701 gcgaaaggaa tttcagacta tgaaggaagc agtcgggata agcaactttt ctctggtcac
     2761 aaaaccgagg ggcaagtgct aatctatgac aggaaggtta aagtttcacc gacacttgat
     2821 gtcccgttac ctgaaaatat tccaagaaaa tattccaagt gtgatttttg tcactgactt
     2881 aatgatgtat aagtgattga attttggcgg agagaggggg atttgaaccc ccggtggagt
     2941 tgcccccact ccggttttcg agaccggtcc gttcagccgc tccggcatct ctccgttcag
     3001 atggttgtca tgatgccagg aaatttggca ttttaacagt ccctgtccgt gcaattttgt
     3061 tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt acaagaatca
     3121 gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt aacgtgtctg
     3181 acagaacagg aacaaagcaa attggtcgct cttgccgaac gttttttaca gcaaaaacgg
     3241 cttgttcctt tacagggctt tgagctgaat tcattaagaa gctgccggat agcacttcta
     3301 ttttgcctgc ccgttctgga gttaggactg gaatggctgg atggttttca tgaagtctta
     3361 atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg tctggtgcat
     3421 aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt tttgaactgg
     3481 ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca tgaagtcgct
     3541 cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat ttcgttgcgt
     3601 gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca ggaagaaatc
     3661 gaattagttg gtgagaatgc ggcgagcatt gatgcttatg ttgccagtga tcctgctgaa
     3721 tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc tcctcgtttc
     3781 ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag atcctttgca gagactgcat
     3841 cacgctaatg atacagactc gttttcagcg acgaatgttc attaattaac aactttgaag
     3901 attaattaac caattgaaat ggcttatgaa atttagtgtt gacagacaag gtaccgctaa
     3961 gtaatatgcg ccccgttctc acgattcctc tgtagttcag tcggtagaac ggcggactgt
     4021 taatccgtat gtcactggtt cgagtccagt cagaggagcc aattttctgt tttcatgcat
     4081 ccttgcgaat ctttatctga tgttgattca acaggttagt gtgaaaactc ttctcggtgc
     4141 attttgattt taccctacgc atcgggaaaa attggtggtc aaatctggga tcaggttagt
     4201 tcgataatgg agtgaccccc atatatccct taccgacgca aaaatccgca ccctcaagcc
     4261 ttctgataaa ccctttaaag tctccgattc tcacggtctg tatctgctgg tcaagccggg
     4321 tggctcccgc cactggtatc tcaaataccg tattagcggt aaagaatccc gcattgcgct
     4381 gggtgcctat ccagccatct ccctgtctga tgcgcgacag caacgtgaag gtatccgtaa
     4441 aatgctggcg ctgaatatca acccggtaca gcagcgggct gctgaacgtg gctcacgaac
     4501 accggagaaa gtttttaaaa acatggcgct ggcgtgacat aaaagtaaca ggaaatggtc
     4561 gcagaacacc gccgaccgtc tgcttgccag cctgaacaat cacatctttc cggtcatcgg
     4621 gaacctacct gtatcagaac ttaaaccccg tcatttcatt gacctgctga aagggatcga
     4681 ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag cacctgagta acataatgcg
     4741 ccatgcggtc catcaggagt taatcgatac gaaccctgca gcaaaccttg gcggcgtgac
     4801 cacacctcct gtcagacggc actatcctgc cctgccgctg gagcggctgc ctgaactgct
     4861 tgaacgtatt ggggcatatc atcagggccg tgaactgacc cggcatgccg ttctgctgat
     4921 gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc cgctggtcag agattgattt
     4981 cacaaaccga gtctggacga tacccgcgac gcgagaaccc attattggcg tgcgttattc
     5041 cggccgcggg gcaaaaatgc gaatgccgca tatcgtcccc ctctcagaac agtccatcgc
     5101 cattctgaaa cagattaagg atatcaccgg taataatgaa ctgatcttcc ccggcgacca
     5161 taacccgtat aagccaatgt gtgaaaacac ggtcaataag gcactgcggg tgatgggtta
     5221 cgacacgaaa aaggatatct gcggtcacgg cttccgggca atggcatgca gtgcgctgat
     5281 ggaatcgggt ttatgggcaa aggacgcagt agaacgccag atgagtcatc aggagcgcaa
     5341 taccgtgcgc atggcttata ttcataaggc agagcacctg gaagcccgca aagcgatgat
     5401 gcagtggtgg tcggattatc tggaagcatg ccgagaatct tatgcaccgc cttatacaat
     5461 tggtaaaaat aagtttatcc catagtatac taacgaatcc cacactctgg caaattacca
     5521 ccacctctac aggaggtggt tctatataga cttttatcaa cttcatgacg aaaaacacct
//
LOCUS       DAEEUE010000179         5559 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.179, whole genome shotgun sequence.
ACCESSION   DAEEUE010000179 DAEEUE010000000
VERSION     DAEEUE010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.179"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..641
                     /locus_tag="KLK84_003931"
     CDS             <1..641
                     /locus_tag="KLK84_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HBE2011058.1"
                     /translation="VGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAITT
                     GKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNA
                     VLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCAL
                     GLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(695..1300)
                     /gene="azoR"
                     /locus_tag="KLK84_003932"
     CDS             complement(695..1300)
                     /gene="azoR"
                     /locus_tag="KLK84_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBE2011059.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1501..5403
                     /gene="hrpA"
                     /locus_tag="KLK84_003933"
     CDS             1501..5403
                     /gene="hrpA"
                     /locus_tag="KLK84_003933"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HBE2011060.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1379 a         1359 c         1539 g         1282 t
ORIGIN      
        1 tcgtaggcgc gtgcattgtg ttgatggagc taatgatgat ggttcagtta tggtggtcag
       61 tctggttatg ttggccagta ttatcgctac tcattattgg ccgtgggtgc ggtgggcttg
      121 gcgcgataac aacagggaaa gatagtcagg ggaaactccc gcccgccgtt tactggctga
      181 cattgccctg gcgcatcggg atgtggctat ctatgcgttg gttttgtcgt cgcctggagc
      241 cggtgagcaa aatgactgct ggtgtttatt taagggcgtt tccacgacat attccggcac
      301 agaatgcggt tctggacgtc acctttgaat tccctcgtgg acgagcgaca aaagatcgac
      361 tctatttctg tgtaccgatg ttggatctgg tggttccgga agagggggag ctccgacagg
      421 ccgtggcgat gctggaaaca ttacgcgaag agcaaggcag cgttctggtc cattgcgcat
      481 tgggattatc gcgcagtgcg ctggtagtgg cggcatggct gttatgttac ggacactgta
      541 aaacagttga tgaagcgatt atctttattc gagccagacg ctcgcatatt gtgcttaagg
      601 aagagcacaa agcgatgttg aaattatggg aaaacaggta agtggattga gatgtggact
      661 gaatatctac agtccacatc aagaccgtgt ccggttatgc agcaacaatg ctgtcgatgg
      721 ctgcttttgc gtcagactgt gctttcgctg ccatttccgg accgtatgcg atcccttcgg
      781 cgaagacaaa tttcacatcg gtaatgccga taaagccgag gaacgtggac agatacggcg
      841 tcaccaggtc cgttggtcca tctttgtgga tcccaccgcg gctggtaata acgatggctt
      901 ttttacccgt taccagacct tccggaccgt tctcggtata gcggaaagta acgcctgcgc
      961 gtgcaaccag gtcaaaatag tttttcaact gagttgagat gttgaagtta tacatcggtg
     1021 ccgcaataac gataacgtcg tgggctttca gctcggcaat cagctcatcg gaaagcgcca
     1081 gagcttcctg ctgacgcgga gtcagcggcg catcgctcgg acgcagagcg ccaaccagtt
     1141 cgccatccag taccggaatc ggatttgcag ctaggtcgcg aacggtgatt tcatcagcgg
     1201 agtgcttttc gctccattgt tcaacaaaat aatcggacaa ctgattagac tgagagtacc
     1261 ctgccaggat gctggattta agaactaata ccttgctcat ggtgtttcct tatagatgtt
     1321 tgaatggacg atgccccgtt gcttgctgac actttattca caatcctgcc acagagatag
     1381 cgcaataaat cgaagtctat gttcgaattt attgaacaac gcatagaaag ccgcgatgtg
     1441 gtactctata tctatcattt aaaagaaaat taatcaggca gactactgcc cactaacgtt
     1501 atgacagaac aacaaaaatt gacctttact gccttgcaac agcggctgga ttcgctgatg
     1561 ctgcgcgaca gactgcgttt ttctcgccgt ctgcattgtg ttaagaaggt taaaaatcct
     1621 gatgcacaac aggccatttt ccaggagatg gcgaaagaga ttgaccaggc ggcagggaaa
     1681 gtcctgctgc gtgaagcggc acgaccggaa atcacttatc ctgacaattt accggttagt
     1741 cagaaaaaac aggacatact cgaagcgatt cgtgatcacc aggtggtgat cgtcgccggg
     1801 gaaaccggct ccggtaaaac gactcagcta ccgaaaatct gtatggagct ggggcgcggt
     1861 attaaagggc tgatcggtca tacccagccg cgtcgcctgg cggcacgaac ggtggccaac
     1921 cgtattgcgg aagagctgaa aacggagccg ggcggttgca tcggttacaa agtgcgtttc
     1981 agcgatcacg taagtgataa cacgatggtc aagctgatga ccgacgggat cctgctggcg
     2041 gagatccagc aagaccgcct gctgatgcag tacgacacca tcattattga cgaagcgcac
     2101 gaacgcagcc tgaatatcga ttttctgctc ggctatttga aagagttgct gccgcggcgt
     2161 cctgacctaa aaatcattat cacttccgcg actatcgacc cggaacgctt ttcgcgccac
     2221 tttaataatg cgccgattat tgaagtctcc ggtcggacct atccggtgga agtgcgctat
     2281 cgcccgattg ttgaagaagc cgatgacacc gagcgcgatc agttgcaggc gatttttgac
     2341 gccgtagacg aactaagcca ggaaagcccc ggcgacattc tgatctttat gagcggcgag
     2401 cgggaaattc gcgataccgc cgatgcgctg aacaagctga acttacgcca taccgaaatc
     2461 ttgccgcttt atgcgcggct ttcgaacagc gaacagaacc gcgtgttcca gtcgcacagc
     2521 ggacggcgca ttgtgctggc gaccaacgtc gcggaaacct cgctgaccgt accggggatt
     2581 aaatacgtca tcgaccccgg tacagcgcgt atcagccgct acagctatcg caccaaagtg
     2641 cagcgtttgc cgattgaacc catttcgcag gcgtctgcca atcagcgtaa aggccgctgt
     2701 ggtcgtgtgt ccgaagggat ctgtattcgt ctctattccg aagacgattt cctctcgcgc
     2761 ccggagttta ccgatccgga gattctgcgt accaacctgg cctcggttat tttgcagatg
     2821 accgcgctgg ggctgggcga tatcgctgcg ttcccgtttg tcgaagcacc ggataaacgc
     2881 aatatccagg atggcgtgcg tctgctcgaa gaactgggcg cgatcaccac ggatgaacag
     2941 gccagcgcct ataaactgac gccgctcggt cgccagctct cgcagttgcc tgtcgaccca
     3001 cgtctggcgc gtatggtgct ggaagcgcaa aaacatggct gcgtgcgtga ggcgatgatt
     3061 atcacgtccg cgctctccat ccaggatccg cgcgaacgtc cgatggacaa acagcaggca
     3121 tcggacgaaa aacatcgtcg cttccacgac aaagaatccg acttcctcgc gtttgtaaat
     3181 ctgtggaatt atcttggcga gcagcaaaag gcgctttctt ccaacgcctt ccgtcgcctg
     3241 tgtcgtaccg attacctcaa ctatctgcgc gtgcgcgaat ggcaggatat ctacacccag
     3301 ttgcgccagg tggtgaaaga acttggcatt ccggtcaaca gcgaaccggc ggagtatcgt
     3361 gaaattcaca tcgccttact gaccggtttg ctttcccata tcggcatgaa agatgccgat
     3421 aaacaggaat ataccggtgc acgtaacgcg cgtttctcca tcttccccgg ttctggttta
     3481 ttcaaaaagc cgccgaaatg ggtaatggtg gcggaactgg tagaaaccag ccgcctgtgg
     3541 gggcgcattg ctgcgcgtat cgacccggaa tgggtagaac ccgttgctca gcatttgatt
     3601 aaacgcacct acagcgaacc gcactgggaa cgggcgcagg gcgcggtgat ggcaacggaa
     3661 aaagtcactg tttatggttt gccgattgtt gctgcgcgca aggtcaacta cagccagatc
     3721 gattcggcgt tatgtcgtga actctttatt cgccacgctc tggtggaagg tgactggcag
     3781 acgcgtcacg cattcttccg tgaaaacctg aaactgcggg ccgaagtgga agagctggaa
     3841 cacaaatcac gtcgccgcga tattctggtt gatgacgaaa cgctgtttga gttctacgac
     3901 cagcgcatca gccacgatgt aatctccgct cgtcacttcg atagctggtg gaaaaaagtc
     3961 agccgcgaaa cgcctgattt gctcaacttt gaaaagagca tgttgatcaa agaaggggcg
     4021 gaaaaaatca gcaagctgga ttacccgaac ttctggcatc agggcaatct caagctgcgt
     4081 ttgagctatc agtttgagcc aggcgcggac gcggacggtg tgaccgtaca tattccgctg
     4141 ccgttactta accaggttga ggaaagcggg tttgaatggc agatccccgg tctgcgccgc
     4201 gaattggtga ttgctctgat taaatcgttg ccgaaaccgg tacgccgtaa ttttgtgcct
     4261 gcaccaaact acgccgaagc gtttttaggc cgcgtcacac cgctggagtt accgttgctc
     4321 gacagccttg agcgcgagtt acggcggatg accggcgtta ccgttgaccg cgaagactgg
     4381 cactgggatc aggtgcccga tcacctgaaa atcaccttcc gcgtggtgga tgacaaaaac
     4441 aagaagctaa aagaagggcg ctcgctacaa gatctgaaag atgcgctgaa aggcaaagtg
     4501 caggaaacgc tgtccgcggt ggcggatgac ggtatcgagc agagcggctt acatatctgg
     4561 agttttggtc agctgccgga aagttacgaa cagaagcgtg gcaactataa agtgaaggcg
     4621 tggccggcgc tggtggatga acgcgacagc gtgacgatca aactgtttga taacccgctg
     4681 gagcaaaaac aggcaatgtg gaatggtctt cgccgtctac tgctgctaaa tatttcctcg
     4741 ccgatcaagt atttacatga gaagttacca aacaaagcca agctggggct gtactttaac
     4801 ccgtatggca aagtgctgga gctgatcgac gactgtatct cctgcggtgt ggatcaactg
     4861 atcgacgcca atggtggccc ggtctggacg gaagaaggct ttgctgcgct gcatgaaaaa
     4921 gtgcgcgccg aactgaatga cacggtggtg gatattgcga agcaggtcga gcaaatcctc
     4981 actgcggtgt tcaatatcaa caaacgtctg aaagggcggg tggatatgac catggcactg
     5041 gggctttctg atattaaagc gcagatgggc gggttggtat atcgcggttt tgtcactggt
     5101 aacggcttca aacggctggg cgacacgctg cgatatttgc aggcgattga aaaacgactg
     5161 gaaaaactgg cggttgatcc acatcgcgat cgtgcacaga tgctgaaagt cgaaaacgtc
     5221 cagcaggcgt ggcagcaatg gatcaacaaa ctgccgcctg cacgtcgtga ggatgaagac
     5281 gtgaaagaga tccgttggat gatagaagag ctgcgcgtta gttacttcgc tcaacaactt
     5341 ggtacgcctt atccgatttc agataagcgt attttgcagg cgatggagca gattagcggt
     5401 taaccctgct atttgcctaa taaatctaaa acccggtaag catttagcgc cgggtttttt
     5461 attaattcta aaacggtaag gacaaaaatt caggaattca gaaaaataca attctcagct
     5521 gcaagatgaa taatgtttat ctacagcatt tccttaaaa
//
LOCUS       DAEEUE010000180         5494 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.180, whole genome shotgun sequence.
ACCESSION   DAEEUE010000180 DAEEUE010000000
VERSION     DAEEUE010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5494
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.180"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            331..537
                     /locus_tag="KLK84_003934"
     CDS             331..537
                     /locus_tag="KLK84_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBE2011061.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            632..1234
                     /locus_tag="KLK84_003935"
     CDS             632..1234
                     /locus_tag="KLK84_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011062.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWGSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            2434..3351
                     /locus_tag="KLK84_003936"
     CDS             2434..3351
                     /locus_tag="KLK84_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011063.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYRHSR"
     gene            3436..4308
                     /locus_tag="KLK84_003937"
     CDS             3436..4308
                     /locus_tag="KLK84_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HBE2011064.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDGLRTESVRGQAREQFTGAVDRIFDTAESVCVASVARKVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            4526..>5494
                     /locus_tag="KLK84_003938"
     CDS             4526..>5494
                     /locus_tag="KLK84_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HBE2011065.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLSTLQQKLDTPAGQQVAQTLQQTGDTCELL
                     DILANDGWLKNEYHGEDEIFTGLASLNDLVRLAAAMGSEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNS"
BASE COUNT         1411 a         1390 c         1332 g         1361 t
ORIGIN      
        1 cggtgatatc agtctcctcc tcgccaggaa cgggctgact cactgtaact acctccgaaa
       61 acaactctgc aattatcaga acgcctgctt ctctccctgt cctcacgaaa actatcccct
      121 ctttatcgcg cgtgcgtgcg gaagcatctt ttcgcaacaa ccacccggta taccggtacg
      181 gctcagccac cgcaacctcc ccgtttatct cctggcggtc acattcccga ttatttttta
      241 cgtttctccc cggttgttat gccggtgaag gtggcgcgct gttttcatga ccacaccggc
      301 tggtttaaca acatccggag gaacattctc atggctaccc cagtttcact gatggatgac
      361 cagatggtcg acatggcatt tatcactcaa ctgaccggcc tgacagataa gtggttttac
      421 aggctcatca gggatggggc ctttccggct cccatcaagc tgggccgcag ctcccgctgg
      481 ctgaaaagtg aagtggaagc ctggctacag gcgcgtattg cacagccccg cccgtaattt
      541 ctgaccgtta tccgttcacc cgcagcagac gtaatccccg gcctgccggc ggcattctgc
      601 tgcctgttat atccccgtga ggaatatgaa aatgaaacaa cagtatcaga cccgctacga
      661 atggctccac gaaagctacc agaaatggct gaccggcttc acccggcacg ccgtatcctg
      721 gggcgtgtgt catccgaata tctactactt ccataatctg acgcccggtt gggggtcatt
      781 caatggtgaa aaaccggaga ttgccatcgt accccagagc ctgcaccggc tgatttatgg
      841 ccctgacaaa cgggccacgc cgcccctgga tgatgatctg atagtgaatt tatgcaccag
      901 tgaacatctg ctggttcatc atccgatgct ggaaggcatt ctcctgtctg aatgtgaacg
      961 tctgaggcag cgttctctgg caaataaact gatcagcctc ttccgtcagt ttggtggcac
     1021 ggagttgcgc ctcaaactgg tctggctttg ctggcttgat ttaatgaccg gaaacagcct
     1081 tgaagactgg aaagagaacc tgaaacggaa atcagaaaaa gagctggagg aatggatcat
     1141 caaccgtcag aggcagagcg cagcactgac ggatctgatg gatcagtacg tgctcctggc
     1201 ataccgcaca acggttgacg atagccgcaa ctgatgccgg ttgcttccgg aaggtgtgac
     1261 aaaatacgga aatgatgatg ctcccggccc ccatgctcac ctgcagagcg tgttcgtcgt
     1321 catcatactg agccatgacg ggcgtcttaa gggcatcctg tcattgctga gcaggcaaca
     1381 ccaccagagg agccacggac agggatgttc tttacagccc gatacacgcc aggttctcag
     1441 cagactgaat gtgcaggtgc ggttattcac cagaaatggc atgctgcggt acatacccat
     1501 cctggtgaca atatgtacgt ggcgacagcg atcccggcac tgtggaagag actcactcag
     1561 cctgcaggat attctttccg gcatccggga atgtccgcct gaatgcaaac aggatgatat
     1621 ttgaatccgt aatactgttc tttcatcata aataatttat gcagatacag ttaaccgtta
     1681 cagtagccgg tttgttttac cgggaaataa tatccgtcag actgataccg gaacatatct
     1741 gaaacacgtt atgatgtttt gttatcagaa atgttgtata ttcttatccg tggcgacagt
     1801 ttaatgccct ctggtatcac ctgataatgt atatcctgcg atgattcgtt atatttacgg
     1861 gacacgcctc ttttcattgt gctccttttc agacttcatt ttccgtaagt gttccccggc
     1921 ataaaggagg tacctttctg gcgtttataa ctctctttat cggtgacgcc ggtaatacag
     1981 ggaataccac accgacaaac gaggtattaa cagacgatat ttcagtgtcc ataatttttc
     2041 tgtccggtta aataacgtca gtctgctgcc ctctgactgt aatcatcaaa gccggaatgc
     2101 ataccgggtt acaacgtaaa aaaagtgatg ctggcccccg gtgcagctgg cgggcgaaac
     2161 atgtttacct ttcaataccc cctgtctgca gtctccactc ttccatcctc ttaagctacg
     2221 tcggccacat gagtgcactt ccatgataac aggtattcct gtcagcataa cgaagccggt
     2281 taccttccct tcctcctgca tttcctgtca gaatgactat acagcgcgca gattgccttt
     2341 ttattgacca tcttaatata ccgttataaa cttcttttta tcatattatt atcaccttat
     2401 tatatttaat ttttaagcag tcggagaaag aggatggttc agaaaatttt gtcggataag
     2461 gttatgaatg aaagaacaaa tgcgtattac tcttattatc ttggagagcg taatatctcc
     2521 gtactgcccc ttaatgttta tgatcccccg gaacgtttta tcgcttacat aaaaaaaaac
     2581 agagaaaatc tgaacatcac actttcagat tttgaacttg aacaaataat ttctggtatg
     2641 cgtctgaagg ccctggcctt cctggtcccc ctggaaaaaa tatcctggat cgcgggcagt
     2701 gaacgcgcat gcttattttc ctggtatctg ttgatgcagt ttatccagaa taacagggca
     2761 aaaataagcg cagacttgct tcaaaagaat aaactgtatc ttaaagaaga gtatctggaa
     2821 gggaatgcct tcccgtcgga ctcatcgaca cagttcagac aaatactcag agttctcgat
     2881 attctttctg ataagaattt gcgggatgag tggataatcc agaccaaaga cagatggatg
     2941 cgggcattta aatcaaagag tcctttcagt tacctcctcc cggagaacga acatgaatgc
     3001 atatggacat ggaattattt gaagggaaaa aatattgcac tggaaaaact ggccagtttt
     3061 cctggttctg ctgatattta ccacgcaatt cacctttcat ttgacatatg ggttacctgt
     3121 ccactcacct cacctgacga tataaaaaat ttcagaaaca gtttcaataa ggccaaggcg
     3181 cagcgcaagt acaaaaaaat gcaggaagat aaagtcaatg tacagttttt tcttgacgca
     3241 gaaaccaggg cgcagctcaa agaattatcg agggtcagaa gactcagtac cggcgaaatg
     3301 ctgcatgacc tgattgttga agagtataaa aggtaccgac attccagata atttcagaca
     3361 tatattatct ccgtgagtcc cccgccacct ttccggtgtg cgggggtttt gtcttttttc
     3421 accgggaata catgtatgaa tccgtctgat gccattgagg caattgaaaa accgctctcc
     3481 tccctgcctt actcactttc ccgtcacatc ctggaacatc tgcgcaaact caccagtcac
     3541 gaacccgtga ttggcattat gggtaaaagc ggggccggta aatcctcact ctgtaatgca
     3601 ctgtttcagg gggaggtcac cccggtcagt gatgttcacg ccggcacccg ggaagtgcag
     3661 cgtttccgtc tgagtggcca tggtcacagc atggttatca ctgacctgcc cggggtgggc
     3721 gagagccggg acagagatgc agagtatgaa gccctgtacc gtgacattct gcctgaactt
     3781 gacctggtac tgtggctgat taaagccgat gaccgtgccc tgtctgtgga tgagtatttc
     3841 tggcgacaca tcctgcaccg gggacatcag caggtgctgt ttgtggtgac gcaggccgac
     3901 aaaacggagc cctgccatga atgggatatg gccggtattc agccttctcc cgcacaggca
     3961 cagaatattc gcgaaaaaac ggaggcggta ttccgtctgt tccggcccgt acatccggtt
     4021 gttgccgtat cggcccgcac cggctgggaa ctggatacgc tggtcagtgc gctaatgaca
     4081 gcacttcccg accatgccgc cagtcccctg atgacccgcc tgcaggacgg gctgcgcacg
     4141 gaatctgtcc ggggtcaggc ccgtgaacag tttaccggtg cggtggaccg gatatttgac
     4201 acggcggaga gcgtctgtgt tgcctctgtt gcccgtaagg ttctgcgcgc tgttcgtgac
     4261 accgtggtct ctgttgcccg cgcggtatgg aactggattt tcttctgaac ccggtggtga
     4321 tgcaccagac tcttgttaca gatttttaac cccatgaaaa cacaatgcaa acacagccat
     4381 acccgggttg tccccttgcg ttgtgtttct caagttaata gaatgcatcg atcgttacta
     4441 tcatatcgat cgctataaac gatcgtttgt ggtaaatgac ctcctccgtg gttgtcatga
     4501 aaatgcgtct caggaaggtt aaataatgca cgaaaagaac atcgccctgc tttgtgatga
     4561 agccgaccga cttttgcaac tgaacattaa tctgctccgg caaatggttg atgaaccgga
     4621 tgtgttatct gacagtaaga acgaaaacag actgcttttt gataaacaga aagcactgaa
     4681 aagaattgag gagctggagg gcgaacaaat caaaaccgcc cgcagggaga tggtgctggc
     4741 tgttgtcggc acgatgaaag caggtaaatc aactaccatc aacgccattg tggggcagga
     4801 aattctgcct aaccgtaacc gccccatgac ctctgtaccg acgctcatcc gccacgttcc
     4861 cggaaaaact gagccggtcc tccatctgga acatattcag cccgtccgca atttattaag
     4921 cacactgcaa caaaaactcg acaccccggc aggacagcaa gtcgcacaga ccctgcagca
     4981 aaccggggat acctgcgaac tgctggatat tctggcaaat gatggctggc tcaaaaatga
     5041 ataccacggg gaagacgaaa tctttaccgg actggcatcg ttaaacgatc tggttcgcct
     5101 tgctgcggca atggggagtg agtttccttt tgatgaatac gcagaagtgc agaaactgcc
     5161 ggtgatcgac gtggaattca gccatctggt ggggatggat gcatgccagg gaacactcac
     5221 actgctagat acccccggcc ctaatgaggc cggacaaccg cagatggaag tgatgatgcg
     5281 ggatcaactg cagaaagcct ctgcggtgct ggcggtgatg gattacaccc agatgaactc
     5341 aaaagcggat gaagacgtcc gtaaagagct taatgccatt gctgacgtat cagccggccg
     5401 cctgtttgtg ctggtcaata aatttgatga gaaagaccgc aatggcgatg gggcagatgc
     5461 cgtacgccag aaagttccgg caatgctgaa cagc
//
LOCUS       DAEEUE010000181         5403 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.181, whole genome shotgun sequence.
ACCESSION   DAEEUE010000181 DAEEUE010000000
VERSION     DAEEUE010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.181"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..497
                     /locus_tag="KLK84_003939"
     CDS             <1..497
                     /locus_tag="KLK84_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HBE2011066.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
     gene            519..2510
                     /gene="cyoB"
                     /locus_tag="KLK84_003940"
                     /pseudo
     CDS             519..2510
                     /gene="cyoB"
                     /locus_tag="KLK84_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2500..3114
                     /locus_tag="KLK84_003941"
     CDS             2500..3114
                     /locus_tag="KLK84_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HBE2011067.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3114..3443
                     /locus_tag="KLK84_003942"
     CDS             3114..3443
                     /locus_tag="KLK84_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HBE2011068.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3455..4345
                     /gene="cyoE"
                     /locus_tag="KLK84_003943"
     CDS             3455..4345
                     /gene="cyoE"
                     /locus_tag="KLK84_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HBE2011069.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVTSGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4530..4841
                     /locus_tag="KLK84_003944"
     CDS             4530..4841
                     /locus_tag="KLK84_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBE2011070.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4825..4983
                     /locus_tag="KLK84_003945"
     CDS             4825..4983
                     /locus_tag="KLK84_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HBE2011071.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAT
                     RIAKEKCA"
     gene            4976..5374
                     /locus_tag="KLK84_003946"
     CDS             4976..5374
                     /locus_tag="KLK84_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE2011072.1"
                     /translation="MRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQP
                     SEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFY
                     ARLGFVQLSATPNAFGAVPMFLAIQHILAA"
BASE COUNT         1181 a         1342 c         1425 g         1455 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaatac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa actatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
      661 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
      721 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
      781 cgggtgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
      841 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
      901 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
      961 ttactgttgt tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
     1021 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg
     1081 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
     1141 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat
     1201 ttacctaggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
     1261 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
     1321 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
     1381 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta
     1441 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt
     1501 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
     1561 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
     1621 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
     1681 tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact
     1741 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
     1801 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac
     1861 tgaacgaaac ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct
     1921 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
     1981 acccgcagtt ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg
     2041 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg
     2101 acctgactgg cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc
     2161 cgttctataa ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga
     2221 aagagaaagg cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa
     2281 acagcggtgc aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct
     2341 ggcatatctg gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga
     2401 aaagcttcga cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa
     2461 accagcattt cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg
     2521 cacgcgactg cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc
     2581 ggattttgga tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat
     2641 gccgttctgg tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg
     2701 ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct
     2761 atcgccatgt acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg
     2821 tttggtgccg gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc
     2881 atggggccgg atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt
     2941 ctgcacgtca cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc
     3001 ggcctgacca gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg
     3061 gatgtggttt ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc
     3121 attctaccga tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta
     3181 tcctgtcgat cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc
     3241 cggccgtaat tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg
     3301 tgtgcttcct gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct
     3361 tcaccgtgct aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca
     3421 actacaacat gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt
     3481 aacgaaacca ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc
     3541 ctcaaagggc agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt
     3601 tgtgacgtcg ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga
     3661 aaggacgaag aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta
     3721 cgccaccttg ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc
     3781 ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa
     3841 acgccactca gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat
     3901 tggctactgt gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt
     3961 cagcctgtgg cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca
     4021 ggcggcaaac attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat
     4081 cacgctgtat atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc
     4141 tgggtataaa tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct
     4201 gcgcggttat aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat
     4261 catcgccatc actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca
     4321 tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct
     4381 tttttattgt ctgagatcag agatacggct aataaccgta tctcttgaca gagtgagcag
     4441 atgcacataa attaatcaaa cggttgatag ccgtattatg tatgagatcg actccctacg
     4501 atttcattca gttaatcaaa gaagtcataa tgcctggaaa aacagccgct ctatacgatg
     4561 tcgataaaac gctgaaaaat gcgcgggtcg aactcaaaac tagtcccgac gccaaaaaca
     4621 aacttcgtga agctgcgcaa gccgttgggg tagatttaag cgcttttatt ttgagcgctg
     4681 ctatggaacg cgccgaaagt gtgcttgata accaacgtcg ccgtgagctt tcgaatcaaa
     4741 gctgggaact gatgaaccaa ctcatcgctg aacctgctca accgacgctc gccctcaagg
     4801 cgttaatgaa aaggaaaaac agcgatggtc gacaagcatg aagagattac tctgcccata
     4861 gtcctctcct gtaattatca gtctgatatt acttatcctg ggcaaaaaca gtttgattgc
     4921 ggtaaccctg ttatcgataa atttgtacgc gctacgcgca tcgctaaaga aaagtgtgcg
     4981 taatagcgac tgtgcggcta aagcacttat tgacagacaa agtggtgaac tgattggcat
     5041 ctgtactttt acggcatatt cgctggaaag acaacgcgtt tctggcgtcc ttcagggttc
     5101 acaaccttca gaaattggtg ttgtcagatt agtcatgttg gggggagcgc ggaagtatca
     5161 aaagcggggc tttggtcagg acctactatg tgattttttt gaacatgtaa aaaaaattca
     5221 cccggcatta ccaattaaag gggtttatct tgatgctgac cctgccgcca ttaactttta
     5281 tgctcgtctc ggctttgttc agctttcagc gacaccaaat gcttttggtg ctgtacctat
     5341 gtttttggcg attcagcata ttctcgcggc ttagattatt atcgccaccc gccagggcaa
     5401 gtt
//
LOCUS       DAEEUE010000182         5360 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.182, whole genome shotgun sequence.
ACCESSION   DAEEUE010000182 DAEEUE010000000
VERSION     DAEEUE010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.182"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            12..398
                     /locus_tag="KLK84_003947"
     CDS             12..398
                     /locus_tag="KLK84_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HBE2011073.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            406..1092
                     /locus_tag="KLK84_003948"
     CDS             406..1092
                     /locus_tag="KLK84_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBE2011074.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1070..1693)
                     /gene="tetR(B)"
                     /locus_tag="KLK84_003949"
     CDS             complement(1070..1693)
                     /gene="tetR(B)"
                     /locus_tag="KLK84_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HBE2011075.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1775..2980
                     /gene="tet(B)"
                     /locus_tag="KLK84_003950"
     CDS             1775..2980
                     /gene="tet(B)"
                     /locus_tag="KLK84_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HBE2011076.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3093..3686)
                     /gene="tetC"
                     /locus_tag="KLK84_003951"
     CDS             complement(3093..3686)
                     /gene="tetC"
                     /locus_tag="KLK84_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HBE2011077.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            3819..4190
                     /locus_tag="KLK84_003952"
     CDS             3819..4190
                     /locus_tag="KLK84_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE2011078.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(4200..>5360)
                     /locus_tag="KLK84_003953"
     CDS             complement(4200..>5360)
                     /locus_tag="KLK84_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HBE2011079.1"
                     /translation="ELHLKRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKR
                     IDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKRLMVLRASVALHG
                     RSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLG
                     WYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKS
                     RSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIE
                     ETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQA
                     NTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT         1464 a          997 c         1174 g         1725 t
ORIGIN      
        1 ggacaaggat aatgtttgat gttcacgttg ttttagataa tcaaatagga caattagcat
       61 tactaggaaa aacattaggt aataaaggta ttggattgga agggggaggg atatttacgg
      121 ttggtgatga atgccatgct cattttcttg ttgaacaagg aaaggaagct aaaatagcgc
      181 tagagcaagc tggactgtta gtacttgcga tccggacacc attaattcgt aagttaaaac
      241 aggaaaaacc gggggaactt ggcgaaatag cacgagtatt ggcggagaat aacattaata
      301 ttttagtgca atacagtgac catgctaacc aactgatatt aataacggac aatgatagta
      361 tggctgcatc tgttacgctc ccttgggcaa taaagtgaac ttgcgatggc taatttaata
      421 cgaaaagagg ttacctttga gtcctcaata gccgcgatag gggcggctat gtctgacatt
      481 tcacgagtta aaatactcag tgctttgatg gatgggcgag cttggacggc cactgagcta
      541 agttctgtgg cgaatatatc agcttcaacg gcgagcagtc atttatctaa attattagat
      601 tgccagctaa tcacagtagt agctcaaggc aagcatcgtt attttcggct agcaggaaaa
      661 gatattgctg aattgatgga aagtatgatg gggatctcct taaaccatgg cgtacatgcc
      721 aaagtttcca cgccagtgca tttacgaaaa gcacgtactt gctatgatca tttagctggc
      781 gaagttgccg ttaagatcta tgattccctt tgtcaacagc aatggatcac tgaaaatggt
      841 tcaatgatca cattaagtgg tattcaatat tttcatgaaa tgggaattga cgttccttcc
      901 aaacattcac gtaaaatctg ttgtgcgtgt ttagattgga gtgaacgccg tttccattta
      961 ggtgggtacg ttggagccgc attattttcg ctttatgaat ctaaagggtg gttaactcga
     1021 catcttggtt accgtgaagt taccatcacg gaaaaaggtt atgctgcttt taagacccac
     1081 tttcacattt aagttgtttt tctaatccgc atatgatcaa ttcaaggccg aataagaagg
     1141 ctggctctgc accttggtga tcaaataatt cgatagcttg tcgtaataat ggcggcatac
     1201 tatcagtagt aggtgtttcc ctttcttctt tagcgacttg atgctcttga tcttccaata
     1261 cgcaacctaa agtaaaatgc cccacagcgc tgagtgcata taatgcattc tctagtgaaa
     1321 aaccttgttg gcataaaaag gctaattgat tttcgagagt ttcatactgt ttttctgtag
     1381 gccgtgtacc taaatgtact tttgctccat cgcgatgact tagtaaagca catctaaaac
     1441 ttttagcgtt attacgtaaa aaatcttgcc agctttcccc ttctaaaggg caaaagtgag
     1501 tatggtgcct atctaacatc tcaatggcta aggcgtcgag caaagcccgc ttatttttta
     1561 catgccaata caatgtaggc tgctctacac ctagcttctg ggcgagttta cgggttgtta
     1621 aaccttcgat tccgacctca ttaagcagct ctaatgcgct gttaatcact ttacttttat
     1681 ctaatctaga catcattaat tcctaatttt tgttgacact ctatcattga tagagttatt
     1741 ttaccactcc ctatcagtga tagagaaaag tgaaatgaat agttcgacaa agatcgcatt
     1801 ggtaattacg ttactcgatg ccatggggat tggccttatc atgccagtct tgccaacgtt
     1861 attacgtgaa tttattgctt cggaagatat cgctaaccac tttggcgtat tgcttgcact
     1921 ttatgcgtta atgcaggtta tctttgctcc ttggcttgga aaaatgtctg accgatttgg
     1981 tcggcgccca gtgctgttgt tgtcattaat aggcgcatcg ctggattact tattgctggc
     2041 tttttcaagt gcgctttgga tgctgtattt aggccgtttg ctttcaggga tcacaggagc
     2101 tactggggct gtcgcggcat cggtcattgc cgataccacc tcagcttctc aacgcgtgaa
     2161 gtggttcggt tggttagggg caagttttgg gcttggttta atagcggggc ctattattgg
     2221 tggttttgca ggagagattt caccgcatag tccctttttt atcgctgcgt tgctaaatat
     2281 tgtcgctttc cttgtggtta tgttttggtt ccgtgaaacc aaaaatacac gtgataatac
     2341 agataccgaa gtaggggttg agacgcaatc gaattcggta tacatcactt tatttaaaac
     2401 gatgcccatt ttgttgatta tttatttttc agcgcaattg ataggccaaa ttcccgcaac
     2461 ggtgtgggtg ctatttaccg aaaatcgttt tggatggaat agcatgatgg ttggcttttc
     2521 attagcgggt cttggtcttt tacactcagt attccaagcc tttgtggcag gaagaatagc
     2581 cactaaatgg ggcgaaaaaa cggcagtact gctcggattt attgcagata gtagtgcatt
     2641 tgccttttta gcgtttatat ctgaaggttg gttagttttc cctgttttaa ttttattggc
     2701 tggtggtggg atcgctttac ctgcattaca gggagtgatg tctatccaaa caaagagtca
     2761 tcagcaaggt gctttacagg gattattggt gagccttacc aatgcaaccg gtgttattgg
     2821 cccattactg tttgctgtta tttataatca ttcactacca atttgggatg gctggatttg
     2881 gattattggt ttagcgtttt actgtattat tatcctgcta tcgatgacct tcatgttaac
     2941 ccctcaagct caggggagta aacaggagac aagtgcttag ttatttcgtc accaaatgat
     3001 gttattccgc gaaatataat gaccctcttg ataacccaag agggcatttt ttacgataaa
     3061 gaagatttag cttcaaataa aacctatcta ttttatttat ctttcaagct caataaaaag
     3121 ccgcggtaaa tagcaataaa ttggcctttt ttatcggcaa gctcttttag gtttttcgca
     3181 tgtattgcga tatgcataaa ccagccattg agtaagtttt taagcacatc atcatcataa
     3241 gctttaagtt ggttctcttg gatcaatttg ctgacaatgg cgtttacctt accagtaatg
     3301 tattcaaggc taattttttc aagttcattc caaccaatga taggcatcac ttcttggata
     3361 gggataaggt ttttattatt atcaataata taatcaagat aatgttcaaa tatactttct
     3421 aaggcagacc aaccatttgt taaatcagtt tttgttgtga tgtaggcatc aatcataatt
     3481 aattgctgct tataacaggc actgagtaat tgttttttat ttttaaagtg atgataaaag
     3541 gcacctttgg tcaccaacgc ttttcccgag atctcatcta ttgaaacagc ttgatagcct
     3601 ttttcaacaa acaatattcg tgctgagtta accagtgatt gataggtact cttaaaattt
     3661 tcttgttgat gatttttatt ttccatgata gatttaaaat aacataccgt cagtatgttt
     3721 atggtatcat gatgatgtgg tcgtgacaat cttaagaaca tttaggttat tttatgtata
     3781 ttgaacagca ttctcgctat caaaataaag ctaataacat ccaattaaga tatgatgata
     3841 agcagtttca tacaacggtt atcaaagatg ttctattatg gattgaacat aatttagatc
     3901 agtctttact gcttgatgat gtggcgaata aagcgggtta taccaagtgg tattttcagc
     3961 ggctgttcaa aaaagtaaca ggggtcacac tggctagcta tattcgtgct cgtcgtttga
     4021 cgaaagcggc tgttgagttg aggttgacga aaaaaactat ccttgagatc gcattaaaat
     4081 atcaatttga ttcccaacaa tcttttacac gtcgatttaa gtacattttt aaggttacac
     4141 caagttatta tcggcgtaat aaattatggg aattggaggc aatgcactga gagatcccct
     4201 cataatttcc ccaaagcgta accatgtgtg aataaatttt gagctagtag ggttgcagcc
     4261 acgagtaagt cttcccttgt tattgtgtag ccagaatgcc gcaaaacttc catgcctaag
     4321 cgaactgttg agagtacgtt tcgatttctg actgtgttag cctggaagtg cttgtcccaa
     4381 ccttgtttct gagcatgaac gcccgcaagc caacatgtta gttgaagcat cagggcgatt
     4441 agcagcatga tatcaaaacg ctctgagctg ctcgttcggc tatggcgtag gcctagtccg
     4501 taggcaggac ttttcaagtc tcggaaggtt tcttcaatct gcattcgctt cgaatagata
     4561 ttaacaagtt gtttgggtgt tcgaatttca acaggtaagt tagttgctag aacccatggc
     4621 tcctttgccg acgctgagta gattttaggt gacgggtggt gacaatgagt ccgtgtcgag
     4681 cgctgatttt ttcggccttt agagcgagat ttatacaata gaatttggca tgagattgga
     4741 ttgcttttag tcagcctctt atagcctaaa gtctttgagt gactagatga catatcatgt
     4801 aagttgctga taggtttcca gttttccgct cctaggtctg catattgtac ttttcctctt
     4861 actcgactta accagtacca acccagcttc tcaacggatt tataccatgg cactttaaag
     4921 ccagcatcac tgacaatgag cggtgtggtg ttactcggta gaatgctcgc aaggtcggct
     4981 agaaattggt catgagcttt ctttgaacat tgctctgaaa gcgggaacgc tttctcataa
     5041 agagtaacag aacgaccgtg tagtgcgact gaagctcgca ataccataag tcgtttttgc
     5101 tcacgaatat cagaccagtc aacaagtaca atgggcatcg tattgcccga acagataaag
     5161 ctagcatgcc aacggtatac agcgagtcgc tctttgtgga ggtgacgatt acctaacaat
     5221 cggtcgattc gtttgatgtt atgttttgtt ctcgctttgg ttggcaggtt acggccaagt
     5281 tcggtaagag tgagagtttt acagtcaagt aatgcgtggc aagccaacgt taagctgttg
     5341 agtcgtttta agtgtaattc
//
LOCUS       DAEEUE010000183         5337 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.183, whole genome shotgun sequence.
ACCESSION   DAEEUE010000183 DAEEUE010000000
VERSION     DAEEUE010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.183"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="KLK84_003954"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="KLK84_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HBE2011080.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="KLK84_003955"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="KLK84_003955"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HBE2011081.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRSGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="KLK84_003956"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="KLK84_003956"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HBE2011082.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="KLK84_003957"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="KLK84_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBE2011083.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1310 a         1289 c         1456 g         1282 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agcggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctatg
      181 cggttaacga cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc acccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggtaaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc cctctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc
     1201 gcgcgaccta gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattac tatccgccca ttgccgatgt accacgttcg ggcatcgtcc atcgtctgga
     1321 taaagacacc actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc gctgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcaacag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc agcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga tgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgaa tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaatattc tgttgcaggt actggatgat gggcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tctgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggttagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggg
//
LOCUS       DAEEUE010000184         5164 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.184, whole genome shotgun sequence.
ACCESSION   DAEEUE010000184 DAEEUE010000000
VERSION     DAEEUE010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.184"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..221
                     /locus_tag="KLK84_003958"
     CDS             <1..221
                     /locus_tag="KLK84_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE2011084.1"
                     /translation="LYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHW
                     GFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(393..722)
                     /locus_tag="KLK84_003959"
     CDS             complement(393..722)
                     /locus_tag="KLK84_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HBE2011085.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(727..912)
                     /locus_tag="KLK84_003960"
     CDS             complement(727..912)
                     /locus_tag="KLK84_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HBE2011086.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(909..3548)
                     /locus_tag="KLK84_003961"
     CDS             complement(909..3548)
                     /locus_tag="KLK84_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HBE2011087.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3756..4745
                     /gene="ldhA"
                     /locus_tag="KLK84_003962"
     CDS             3756..4745
                     /gene="ldhA"
                     /locus_tag="KLK84_003962"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBE2011088.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4856..>5164
                     /gene="hslJ"
                     /locus_tag="KLK84_003963"
     CDS             4856..>5164
                     /gene="hslJ"
                     /locus_tag="KLK84_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HBE2011089.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     N"
BASE COUNT         1305 a         1323 c         1205 g         1331 t
ORIGIN      
        1 gtttgtaccg aatgtttgcc gataaaggtt tggtagtcgc gcaatatatt cgtaaccgtc
       61 gtctcgattt ttgtgcagat gcgattcgcc atgccgcaga tgatgaaaaa ctggcaggca
      121 tcggctttca ttggggattt tctgaccaga gtcatttttc aacggtattt aagcaacgct
      181 ttgggatgac gccaggcgag tatcgacgta aattccgcta actaattggc gttgcatgta
      241 ctgcaacgcc aattagttaa aacagatatt tgactatgca tttttcaggg cgaaaggcaa
      301 tttgacagga gtttttgaat attcaccttg tttcaggaaa atagatcaat cgctttctcc
      361 tgtagcgtta tcccgatatt gcggttttgc tattaaaact ttcgcaccca tttaccgttt
      421 atcccttgta catattcccc cggtttggcc cgagctacga gcttttgccc ggcgagtttc
      481 gcgatatcat ctacagacac attattttgc tttgcccgtt gttggtaact cgctttacgt
      541 tcggcattaa tttcggttac taatttctct gtttcagcat ccgttttcag cgcaaccaga
      601 taaccataaa atgtttcacc tacccgcccc tgagttctgg cttcatccag agtcaatgcc
      661 attacattgc tgcttaccag cccaacaaga aacgcacaaa gaagtaatgt tttcttcatc
      721 atcgcctcag aaaagatcgc tacgagtttc aagcagctct tcgacatctt tgtctgcctt
      781 gatgatgatc tcatgctcaa ttttaacgtt catattgata gtgatcggtt ccttaggtgc
      841 agcgacttca atgcgaggcg tacagccaac cagcataaaa gatgacgtca acgcagccag
      901 taaaattttc attgtttttc ctcacactct tttccttgcg gacagtcatt tcccggcaga
      961 cgtgcgttct gctccaacca tgcctggaga ttatcgccaa agcgtaaact gcgccacagc
     1021 gtaaaaatat tctcttcatg atggtaatta aggtttaccg taccgctttt accatcaacg
     1081 cgactggtac ctgtaatgtt ggcctgcatg gttaataaac cgagattatc taagttaatt
     1141 tttgtcgatg accggctaat ttccatatag cgcaaccagt taattgctga acccgcagtc
     1201 atattgtctt tcaccaccgc atctgcggta tctttatcca gccgcaaggt cattggcccg
     1261 ctatttgcca gccaaccatc tttcactatc catttttcgt tgtttagcca taacggcaat
     1321 gcaccactaa atgccccgga catggcgaat tgtttcggat tgacggcgct aaccagttcg
     1381 ctggatgaga gattattcag ccgcaacagc gccgggtcat gttgcggcat acgtaattgt
     1441 ttcatcagca cgttaccgcc taacacatcg acgctaacat cagtcaacag caagggttct
     1501 tcttcagtcc acggataacg cccttgcaaa tcagccgtaa tatttttcgc tgtcaccaga
     1561 ttaatcactt cggcaattcg caacgtaacg gggccgcgag tccccagatg ccaggctcca
     1621 tcggcaaaac ggaaaggcag gacaaaatcg acaccgttaa cctgattatc tggcatccag
     1681 gcactaccgc ctttcaacac gccgtgccct cccgcgcgga atccttgttc aggcgcagca
     1741 gaaaatgcaa cctgagcata cagttcacca tcgcgtaagt tcatcttcca gtcgggtggc
     1801 accagcggct ggaatacagt cagtgattgt tttggccacc aggcgttacc gcgcagacga
     1861 ataccgtccc agcgaccatt tacccgaacc gggccaatct caccagcatg taaatcgcct
     1921 ttaaagagga aataggtcgg attgcgccca tcgacgctaa attttaaggt tgatggcggt
     1981 aacacactgc cgccagtgaa cagcgtttgc ccggcgtcca gtgacagcgc gccgctgaag
     2041 gagggatgct gtgcgtcacg tacccagacg acgggcttgt cgagaattaa tcgcggcttt
     2101 tctaccgtca tcgtaccgta ttgtaactgg tcgaaaccgg tggagagatc ggtcagcgta
     2161 atcgtgctgt catgccactc accttttcct gcgacatccc aggtggcatt catcggtgta
     2221 aaactccctt ttccccagta gcgccactgc cagcggccag cgtcagggag aaaatcattc
     2281 gccagcccat ccatatgcag cacgaaatcg cccagttcat tttcatgcgc ctgcaaaatg
     2341 gcctgcaaac gtccgtcaac accacgttgg gtgactttta cacccgctaa aggccagcgg
     2401 atttcatcga tgtccagcga atcgatgact cttccctttg aacgaagtaa cgcgcctggc
     2461 tcaaaggtca gcgttgggtc agacagactt ccacttagct gcgcaggtaa acgggcatat
     2521 aaaatgaggt ccgcctgttt cgcttcaccg gtcagctgca gaggcagttg actgttatcc
     2581 atgcttaatt ttcctgggcc aaaattaagc accgcgttgc ccttacccgc ttgcccctgg
     2641 gtcagcacat tcagtcgtcc gctgaccaga gcgttctcaa gccctgcctg ccagttgtcg
     2701 actttgacac ccagtcggcc actcaaagga aaacctgcat acagccagct ccagcgacca
     2761 tcgcttacgg tcaattgttg acgagtaatt tgccacggca aatcgagcaa cggatcgccg
     2821 ttatcccgtg ccagcacgat caattgcccg ctattttcct gccagtccag ctcggcatcc
     2881 accagtgacg gttcctgcgg caagtttaac gtcgcagtag catgcccact tacaggaagt
     2941 ccatccggca cgagcggcat agtaaattcc cccaccagtt taaccggcgg ctgattttca
     3001 aacgcgacga catccagttc gctgactgta agttgttgcc ctttcagctg gccttgaaat
     3061 ttaacttttt cgccctgata acgcagttgc tggatatcag aggttaatgc gagagagagt
     3121 tttccctgcc attcctgcca gggagaaaaa atcagtttat cgatattgat ccaggtgtta
     3181 ggcagcatgg actgccactg cgcgagggtt tttggagcag ctggcgattg ctccgtctgc
     3241 ggcaatttcg ccagacaagc agaatcaagt tctaccgtgc cgacgttcaa taaccagcgg
     3301 ctgggatgtg aaaggctggc gttggtgata tgcgcaagct gacaatctcc caccagataa
     3361 cggagatcgg gaatgattaa acctttacgc gtaatgcgtg ggctttcatc taatgcaata
     3421 cgtgtcccga gcggtagcca gatgtccgcc agcgtgggaa cccacagccc gagcgtcatc
     3481 agcagcgtca acggcacaag aataatcagt aataacagcg cgagaacggc tttatattta
     3541 cccagcatgg gtagttaata tcctgattta gcgaaaaatt aagcattcaa tacgggtatt
     3601 gtggcatgtt taaccgttca gttgaaggtt gcgcctacac taagcatagt tgttgatgaa
     3661 tttttcaata tcgccatggc tttcaattat atttgaaatt ttgtaaaata tttttagtag
     3721 cttaaatgtg attcaacatc actggagaaa gtcttatgaa actcgccgtt tatagcacaa
     3781 aacagtacga caagaagtac ctgcaacagg tgaacgagtc ctttggcttt gagctggaat
     3841 tttttgactt tctgctgacg gaaaaaaccg ctaaaactgc caatggctgc gaagcggtat
     3901 gtattttcgt aaacgatgac ggcagccacc cggtgctgga agagctgaaa aagcacggcg
     3961 ttaaatatat cgccctgcgc tgtgccggtt tcaataacgt cgaccttgat gcggcaaaag
     4021 aactggggct gaaagtagtc cgtgttccag cctatgatcc agaggccgtt gctgaacacg
     4081 ccatcggtat gatgatgacg ctgaaccgcc gtattcaccg cgcgtatcag cgtacccgtg
     4141 acgctaactt ctctctggaa ggtctgaccg gctttactat gtatggcaaa acggcaggcg
     4201 ttatcggtac cggtaaaatc ggtgtggcga tgctgcgcat tctgaaaggt tttggtatgc
     4261 gtctgctggc gttcgatccg tatccaagtg cagcggcgct ggaactcggt gtggagtatg
     4321 tcgatctgcc aaccctgttc tctgaatcag acgttatctc tctgcactgc ccgctgacac
     4381 cggaaaacta ccatctgttg aacgaagccg ccttcgatca gatgaaaaat ggcgtgatga
     4441 tcgtcaatac cagtcgcggt gcattgattg attctcaggc ggcaattgaa gcgctgaaaa
     4501 atcagaaaat tggttcgttg ggtatggacg tctatgagaa cgaacgcgat ctgttctttg
     4561 aagataaatc caacgacgtg atccaggatg acgtattccg tcgcctgtct gcctgccaca
     4621 acgtgctgtt taccgggcac caggcattcc tgacagcaga agctctgacc agtatttctc
     4681 agactacgct gcaaaactta agcaatctgg aaaaaggtga aacctgcccg aacgaactgg
     4741 tttaatcttg cagctcccct gcaatccagg ggagctgatt cagataatcc ccaatgacct
     4801 ttcattctct attcttaaaa tagtcctgag tcagaaactg taattgagaa ccacaatgaa
     4861 gaaagtagcc gcgtttgttg cgctaagcct gctgatggcg ggatgtgtaa gtaatgacaa
     4921 aattgctgtt acgccagaac agctacagca tcatcgcttt gtgctggaaa gcgtaaacgg
     4981 taagcccgtg accagcgata aaaatccgcc agaaatcagc tttggtgaaa aaatgatgat
     5041 ttccggcagc atgtgtaacc gctttagcgg tgaaggcaaa ctgtctaatg gtgaactgac
     5101 agccaaaggg ctggcaatga cccgtatgat gtgcgctaac ccgcagctta atgaactcga
     5161 taac
//
LOCUS       DAEEUE010000185         5154 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.185, whole genome shotgun sequence.
ACCESSION   DAEEUE010000185 DAEEUE010000000
VERSION     DAEEUE010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5154
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.185"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..407
                     /locus_tag="KLK84_003964"
     CDS             <1..407
                     /locus_tag="KLK84_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HBE2011090.1"
                     /translation="KWGEIAPFTVTPEMLEAAREARRQEIEAWRAEQEAKLFTFEWNG
                     RIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWSDADNQQVKLSMPELEELAAAMVQAQV
                     DRNDEIYRRQRELKEELNSLKDLNSVRNFIVE"
     gene            complement(608..1465)
                     /gene="sitD"
                     /locus_tag="KLK84_003965"
     CDS             complement(608..1465)
                     /gene="sitD"
                     /locus_tag="KLK84_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HBE2011091.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1462..2319)
                     /gene="sitC"
                     /locus_tag="KLK84_003966"
     CDS             complement(1462..2319)
                     /gene="sitC"
                     /locus_tag="KLK84_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HBE2011092.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2316..3143)
                     /gene="sitB"
                     /locus_tag="KLK84_003967"
     CDS             complement(2316..3143)
                     /gene="sitB"
                     /locus_tag="KLK84_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HBE2011093.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(3143..4057)
                     /gene="sitA"
                     /locus_tag="KLK84_003968"
     CDS             complement(3143..4057)
                     /gene="sitA"
                     /locus_tag="KLK84_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HBE2011094.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4519..>5154)
                     /locus_tag="KLK84_003969"
     CDS             complement(4519..>5154)
                     /locus_tag="KLK84_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBE2011095.1"
                     /translation="PLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVD
                     FEKGIVNVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHHRKSEITFY
                     HREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHT
                     FACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1371 a         1256 c         1247 g         1280 t
ORIGIN      
        1 gcaaatgggg tgagatagcc ccattcactg tgacaccaga aatgctggaa gcggccagag
       61 aggccagacg tcaggaaatt gaagcatggc gcgcagaaca ggaggcgaag ctgttcacgt
      121 ttgaatggaa cggtcgtatc tggaatgctg gtcccgactc actgggccgc ctgtccccgg
      181 tagtcatgct ggcaaaatct gtcacagcac aaacacatat ggcgtggagc gatgccgata
      241 atcagcaggt gaaactgtcg atgccggaac tggaagaact ggcggcagca atggtgcagg
      301 cgcaggtcga tcgcaacgat gagatttatc gccgtcagcg ggagctgaag gaagagctga
      361 atagtctgaa ggatttgaat tcggttagga attttatcgt ggaataacag aagctgcggc
      421 acgtcgtatg caggaacgtg ccgcggttgg ctggtaaact ttcgatagtg cgagtattga
      481 atgatttcta ggcgttatcg attttacgta ttttttgcat gatagaattc gtacctcctc
      541 ccaacgacct tccatggctt tccggacttc tgtaggctgg cggacgatgc tgtcctcgga
      601 tgttgtgcta attgggtgat aacagatcct gtgtatgggc gtttcccttt gtgcgtgcgt
      661 tgatgctggt aacggtaaac gtcatgatga acacgaccgc gaataagacg acaatcgtcg
      721 gagctggtgc gctgtcgata aaaaaggaca gccagacacc agtcattgac actattaccg
      781 atatgccagt cgccagtagg agcgcaatat gaaagcgttg tgtcaggagt actgcaatgg
      841 cgccaggcgc aatgagcaga gaaatagaga gaatgatacc tactgctttc agcgtcgcca
      901 caatggtcag ggaaaccatg cacagcagcc cataatgcag ccatctcgta tgcaaaccgc
      961 ttacctgcgc ctgttgataa tcaaagctga acagcaaaaa atcccgccat tttacgctaa
     1021 taacaagtgt aaccagcccg gcaataatca ccgtctggat tatatcgccg atggttatcc
     1081 cgagcatgtc gccgaaaaga atatggtcaa gatgtacgtc tggtttaatt gcaatataca
     1141 agatcagacc tgcggcaaac atgccagaaa aaacaatccc cataactgta tcttgcttga
     1201 ttcggctgtt gtctttcagg tatcccgtcg ccaccgcgca gaatactccg gcgacgaatg
     1261 cccctgtagc cagaggtaac cccagaatcc aggtaagcac gatgccagga aaaaccgcat
     1321 gactcatcgc gtcacccatc agtgcccaac ctttcaggac cagaaagacc gacagcagcg
     1381 cacagggtat ggaaactacc agtgaaatca gcagtgcgtg actcataaag gtgaactgca
     1441 gtggctccag taataatgcc atcatgagcg atcctccagc gctttatgcg cacgccgacg
     1501 atttgccaaa agtccatggg tgggcgcaaa tacaaatgct aaaagaaaca gcagtgtctg
     1561 cgccaccacg atgattccac ctgtcgcgcc gtccagaaag taactgaccc acgcgccgag
     1621 gaaactggtt acactgccaa tagttacagc aattatgagc aggcggggaa aacgatcggt
     1681 taacaaccat gctgttgcac cgggagtcac caccagacag atcaccagaa acgcaccaac
     1741 cgtttgcagc gccgcaacgg tcgaaaccga cagcagggtg aagaagagga tttttaatct
     1801 gcccggatgt aatccaatag cacgggcgtg attctcgtcg aaaaaggtca ccataagatc
     1861 tttccatttg aaaaaaagca cgatgatcga gagaatgcca atgatagtga gttgcaggat
     1921 atccgccgga tcgattgcca gaatattgcc caggacgatg gtctgaatgt tcacggatgt
     1981 cgggttcaat gacaccataa acagccccag gccaaaaaag gacgagaaga tcaggccgat
     2041 gatggcatct tcttttaagc gggtgcgttg gttaagaaac agcatgctgc ccgctgccag
     2101 tccgccggaa aagaaggctc ccagagaaaa cggaagcccc agcatatatg cccccgcaac
     2161 acccgggaca atagagtgcg acagcgcatc gccaatcagc gaccagcctt ttagcatcag
     2221 atagcatgac aggaaagcgc agagaccgcc gaccattgcc gagacccaca tcgcattgag
     2281 catatattca tagctgaagg gttccagtag cacgttcatc ttgcttccct ctgcaccgct
     2341 gacggtcgat gtgccacaaa agggcgttca tcgtcagtaa taatgctttc ttccgagccg
     2401 ttaagtgtga catggcgcag tacgccgcta aaagccagct ccaggttctc ggctgtaaaa
     2461 gtggtgtctg tcggcccgct tgccaggacg gtgcctttga ccatgaccgt ataatcgcaa
     2521 aatgtcgtga ctgagccgag attgtgcgtt gagacaagca tcgttttgcc ttccgcgcgt
     2581 aactccctca acaggctgat gattttcgct tcggttttca catcaacgcc ggtaaaaggt
     2641 tcatccagca ggatcacatc accctgctgt gcaattgctc tcgccagaaa gacgcgtttc
     2701 ttttgtccac cggaaagctc gccgatttga cgatggcgaa aatccaccat atcgacacgt
     2761 tccagcgcat cggtgacaat ctgacgatcc ctctttttgg ctatacgcaa catacccata
     2821 tgcccatagc ggcccatcat caccacatct tccaccagta caggaaatga ccagtcaacc
     2881 tcttctgact gagggacgta ggcaaccagg tttttctgta gcgcctgtcg cgtgggaata
     2941 cccagaacag atatctttcc gctggtcaga cgcacaaatc ccataatcgc tttaaacaac
     3001 gtcgattttc cggaaccgtt taccccaacc agagcggcaa tagatccact tggtactgtg
     3061 aaggatgcat cgcgcagtgc tgtgtgcccg ttacgccagg tgacagtaac atcattaacg
     3121 acaatgcctg cagattgcat cattatttct cccttttccc ggctttaatt ccctgtacca
     3181 gcgtactggt agtaacctta agaaggtcga tatacgtagg taccgggccg ttttctgtgc
     3241 tcagggaatc gacatagagc acaccgccgt agtgtgcgcc ggtttcacgc gcaacctgac
     3301 gcgctggttt atcggaaatc gtactctcgc taaagactgc cgggatatga tttttcttaa
     3361 ctatatcaac aaccttacgt acctgctgcg gtgttccttg ttgatcggca ttaatcggcc
     3421 acagataaag ctcttttagc cccaaatcgc gtgcgagata agaaaaagcc ccttcactgg
     3481 tgaccatcca tcgctgattc tcagggagtt ccgtaatctg cttacgcagg ggggcaaggg
     3541 tttgggtaat cttggcttta taagtatcgg cattacgttg gtaggtttgt gcatttgccg
     3601 gatcgtattt tatcaacgca tcacgaatat tatcgacgta aatcagagca ttatctggcg
     3661 acatccatgc atggggatta ggtttgccct catagggtcc ttcggtgatc cctactggcg
     3721 tcacacccga agagacaatt acttctggaa ccccattgag atgctggtaa aagcgttgga
     3781 accacaattc cagattcata ccattggcga gaatcagttg tgccccctgc gcacgtttaa
     3841 tatcgccagg ggtaggctga tactcatgaa tttctgcacc aggcttggtt atggatgaga
     3901 cttctgcagc atctccagcc acgtttttgg ccatatccgc gatgatggta aatgttgtaa
     3961 tgaccttgaa tttttcagtt gcacttgcct gaaatgcgat cgagcaggtg agggcgaggc
     4021 accccaagag catggttact ttttttattg agtgcataat ttagtaccta tagtgagcaa
     4081 aattagaaca aacatagcaa cggctatgtt ttatagcata acctatacaa aatgaaaata
     4141 attatcatta acagctttga ggcctaaaac agggattagg ttgtttcctg ctggcgacat
     4201 gatattgagg aaaaaagtag agcagctatt aggctttgaa atgactttat gaggaaaatt
     4261 gttgatggca aaaatggggc aaaacgctgt ataaggggca aaaaaggggc aaaaaagagt
     4321 gggttatcgt agcttattgt tgtcgctgat aatgtttaac atattgaaaa ataaataaaa
     4381 tacttatgag tcagatagtt gtgatttttg cccttacttg ttcaggtcgt attgttcttt
     4441 ctttgtaatt tgttgatttt cttgcattat ttcagttctc tggtactaaa tggggcaaat
     4501 tgggggcaaa ctttgcaact acgataaccg cgcattcaac atggctatct gttcgtcgtt
     4561 catgtcatca atccacatac cgtaaatttc atacaccatt tgcgcagttt catgccccat
     4621 ctggctggct ataaatgccg ggttcgctcc tgccgtcaac agccagcagg caaaagtatg
     4681 tcgcgtgtgg tacggattac ggcggcgaat accagcacgt tttactgctg cattccatct
     4741 tgtacccaaa ctgcttaccg agtaataagg tttctgtttt tcgttacaca ccctgggcat
     4801 gaaaacaaaa tgcagttttt gtttttcagt tctgccgtac tcccgatgat aaaaagtgat
     4861 ttcgcttttg cgatgatgcc cggtcagttt gtattgctcc ttcagtgctt caagagccgg
     4921 ctgcagtaat gttaccgtcc ggatcccggc atttgttttt gggggaccga acatatcaag
     4981 tatcgtcagg tttcttctga cattcacaat tcccttctcg aaatccacat cctcccacgc
     5041 cagagctgcc agttccccgt gacgaagccc ggagtaaacg gcaaatttcc acaagttttg
     5101 gctctgtcct ttttcacttt ccattaatgc attgaattct gttttagata acgg
//
LOCUS       DAEEUE010000186         5152 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.186, whole genome shotgun sequence.
ACCESSION   DAEEUE010000186 DAEEUE010000000
VERSION     DAEEUE010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.186"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            188..445
                     /locus_tag="KLK84_003970"
                     /pseudo
     CDS             188..445
                     /locus_tag="KLK84_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(495..1445)
                     /gene="uspE"
                     /locus_tag="KLK84_003971"
     CDS             complement(495..1445)
                     /gene="uspE"
                     /locus_tag="KLK84_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HBE2011096.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTTMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1597..2349)
                     /gene="fnr"
                     /locus_tag="KLK84_003972"
     CDS             complement(1597..2349)
                     /gene="fnr"
                     /locus_tag="KLK84_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HBE2011097.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2544..3059)
                     /gene="ogt"
                     /locus_tag="KLK84_003973"
     CDS             complement(2544..3059)
                     /gene="ogt"
                     /locus_tag="KLK84_003973"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBE2011098.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3070..3213)
                     /locus_tag="KLK84_003974"
                     /pseudo
     CDS             complement(3070..3213)
                     /locus_tag="KLK84_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3603..4418)
                     /locus_tag="KLK84_003975"
     CDS             complement(3603..4418)
                     /locus_tag="KLK84_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE2011099.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4515..>5152
                     /locus_tag="KLK84_003976"
     CDS             4515..>5152
                     /locus_tag="KLK84_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBE2011100.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLV"
BASE COUNT         1375 a         1173 c         1226 g         1378 t
ORIGIN      
        1 tattttaccg cgacaattca ttcagaacat caatagtcag ggaaggaagt agcaacatta
       61 gctaactaat caatgcgttg attgtaaatc cagctaagag gtgaggtttt cagagcagac
      121 aacggtgaaa cgtcatggta ttgttacgtt taggtaacaa gaaatttgtc tgcacaagga
      181 ttacatcatg attatggcga aactgaagtc agcgaaaggg aagaaatttc tctttggttt
      241 gttggcggtt ttcattattg cggcgtcggt tgtgactcgc gcgaccatcg gcggcgttat
      301 agaacagtac aatattccgc tgtctgagtg gacgacatca atgtatgtga tttagtcatc
      361 gatgattttt gtttatagcc tggtctttac tgtgttgctg gcaatcccgt tgggaattta
      421 tttccttggc ggcgaagagc agtaagtaaa aaataggccc gataactcgg gccttgtcag
      481 ttattgaaga gtcgttaatc gtcttcttcg tcatccagtt caacgggtgt ctgatactgg
      541 tcaggtttaa tgaccagcag gtcgcagcga agatgatcaa tcacctgttc cgccgtgttg
      601 ccgaggaatg ctgctgaaat accggtgcgt ccgaccgtgc ccagaaccac aattcccgcc
      661 tgtaaatgct ccgccaaatc aggaatcacc tcttcgggca gacctttttc tacgtgcgtc
      721 atgttttcat taatgccgaa tttctgccgc agggctttca ttgccagcaa atgttgccca
      781 cgaatggcat cgttataaac gctcgggtca aattccggca gttcaatcgc gatattaatt
      841 ggcgttaccg gataagcgcc aaccagatga acttcggtat ggttgacttg ttctgccagt
      901 tcgatcgtct ctttgaccag tttttcattg agcgcattat gatacggctc ttcactggcg
      961 agattcaccg ccaccagcgc cttgcctcct tccggccacg gctggtcttt caccatccac
     1021 accgggcttg ggcatttgcg taacagatgc cagtccgttg gcgtaaaaat caccgcttcc
     1081 agacggtcat gttggtgcgc catttttagc accaaatcgt gtccgccgct gatcacttcc
     1141 tgaatgatgg cttcgaaagg acggttatgc cagaccactt taatttcaat gggaacgcca
     1201 gcattgagat aatattttgc ctgctcgtgg atccaggctg tacgctggct gatgacgccc
     1261 tgacgcatag tggtacgttc gtccggggag agcagggtgg tcatttcgta tgagaagtca
     1321 tagatcggca aaaaggcttt aattttgcca ccaatccgtt gatgtaaata aacagctcgc
     1381 cgcaatgctg gttggtcgtc ctggttagga tcgataacaa cgagcatgtt ctgatacata
     1441 gccatacagg gtctccttac aacaactgtc aacgcagttt gtaattaaaa gattaaccca
     1501 tatctggtga atgaaacagt gatgaacctt ctgccagatc aataaatcag aaaaatttaa
     1561 tgatatgaca gaaggatagt gagttatgcg gaaaaatcag gcaacgttac gcgtatgacc
     1621 agcaagctgg gccagcgcat cgttattttc gatggtgatg tatttacctt tgactgccag
     1681 cataccgctt ttctggaagc gacccagcag acggctgatg gtttctaccg tcaggcccag
     1741 atagttacca atatcgccac gagtcatcgt caggcggaat tcacgagggg agaagccgcg
     1801 ttgggcaaaa cgacgggaca ggttgtagat gaatgcagcc agacgttcct cggcattttt
     1861 cttcgacaac agcaagatca tgtcctgatc gcctttgatt tcaccgctca tcagacgcat
     1921 catctgctga cgcagattcg gcattttacc ggacaaatcg tccagcgttt cgaacggaat
     1981 ttcacatacc atcgaggttt ccagcgcctg agcgaagctc gggtgatggc cgctgccgat
     2041 ggcgtcaaat cccactaggt cgcctgctaa atggaaacca gtgatttgct cgtcgccttg
     2101 ctcagtgatt gtataacttt taatcgtacc ggagcggatg gcataaagcg atttaagttc
     2161 atcaccagcc ttaaacagcg tctggccttt ctgaataggc ttcttccgct caatgatatt
     2221 atcaagctga tcaagctcat gttcgttgag tgtgaacggg atgcaaagct ggctgatgct
     2281 gcaatcctgg caatggatag cacaaccgcc agactgaatg cgccgtataa ttcgcttttc
     2341 cgggatcata ggtctgctca agccgtaatt gatatttgtc aattttaaca tctttttagg
     2401 gagcaagtaa gtctaagcaa accttaacag cagagaattc cgatattaga tgtaaatata
     2461 tgtctatcta tttgaaactc cttaagttgt caagggtaac tttacataaa agtgtgaaca
     2521 agctggcaca aattgtttaa tgtttacagc aaaagataac cttcgtggcg caataaccac
     2581 tcttttcgct gaactccgcc tgcatatccg gtcatggtgc cgtttcggcc aataacccga
     2641 tggcaaggta cgacgatgct gatgggattc gatccgtttg ccgcaccaac ggcacgcgcc
     2701 gcgccagggc ggcccaattg ttcagccagt tggccgtaat gcattacctg cccgcagggg
     2761 atagtgcgta gtgttttcca gacttcgcgc tgaaatggcg tcccccccgt ggcagtggga
     2821 agcgtatcaa taatgctaag attaccggca aaatattcac gaagcttgtc gcttaaacca
     2881 cctgggttgg tggcagaaat gcgctcatag ccttctttgc gataatggat gtccagcagc
     2941 tgcaccatgc gttcgctgta ctcttcccat tcaaccgccc gcaggcgaaa ttgctcatcg
     3001 caaatcaccc acagtggacc cagtggcgtg gcaatttttt cttcaagtaa tctcagcatc
     3061 cgttctctct taagacaaac gtgggtaaat acccggacct atcggcagac cgacaagata
     3121 ccacgccagc aacatcagca gccataccac caaaaagata agtggatagg gcaagactaa
     3181 cgaatagtaa gtacccagtt tcgcgtctgg tttgtcgcag tctgatagca taaagcgatt
     3241 ttttaatttt tctgcctgaa gaaatgaggg caagccatca catggatcga gggtgggata
     3301 attccagtca gtgaaatttt accgtaggct atctgttatt tgattctgtg gcgtgatagc
     3361 cgccagacac cactgaactc gtcactcatt ccccaatccc ggcgagaatg tggttattta
     3421 cagagctaaa aaatgaataa aaaaatagag cctgtcgaca tccgccagac tctacagtac
     3481 acacagcagt gcatcctcgt cttaaatccg gcctggggtc ctggtgaccg gtgacagtgt
     3541 tataacggtt ttacccatcc gttgccaatt ttcttcagtg acagatttca cgaaaatata
     3601 agttaaatca tttgtcttgt gattgcataa ttatgcatcg ttaatcgacg agtaaattta
     3661 ccgggcgagt agcccgttcg acgcttaaac atattgccga acgattcagc tgaggcatag
     3721 cccagacgat gggcaatttt ggtcaacgaa tatccatcat caagaagcgt cactgcagaa
     3781 ataacgtgca tttgctgaac ccagtggcga aatgttaatc ccgtctcttt aacaaataag
     3841 cgcatgagtg tacggctact gacgttaatt ttatccgcta gattatccag tgttggcaga
     3901 tacccttggt tgtttagcaa attctcacaa agctgctgta gccgctcatc atttggccaa
     3961 gggagttcaa gtgacatttt cacccccatt tttatttcct gaagtatcag tgtctcgagg
     4021 gctgaaaaac gtaactgttg tttatctgtg gtactttgcg acggtggaag ttggttaatt
     4081 gcaattagta attcccttaa caggggtgaa atacccgtca tgaagcaaac tgttcccagc
     4141 gtttcacttt ccgcttctga aaccagtacg gtttttaaaa gtacgttgcc tgtcgcaaca
     4201 aacttatgcg gcatttgtgg gggcagccaa agcgcgctgc tggcatggac tagccagacg
     4261 ctttctccag cccatacacg aacgactcct tcagccgggt aaagcaactg ggcatagaga
     4321 tgtttatgcc agggaactgt acctccattc agatgttccg aatccagtcc gataatgtga
     4381 tgccctggtg atgctttggg ttgcagtgta attgtcattt acagtaggta ttttgtcaga
     4441 aatcatcaaa taagccatgt taaattttaa atatgacgta tgcaagtttc cggatatata
     4501 cggtgtaaag agtgatgata atttctaaaa aacagcttat tggggtggta gcgatcggta
     4561 ttcttttgac tggcgttgtc ttttttatct ggagggtaag caaaggaaga tttatacaga
     4621 cgacggatga tgcttacatt ggtggtaata tcaccacggt ggcaagtaaa gtttctggtt
     4681 atatttctgc tatagaggtg cgcgataatc agagcgtaaa aaaaggcgat attatcctgc
     4741 gtctcgatga tcgggattat cgtgcaaatg tcgccaggct tgaggcaaaa ataaaaagta
     4801 gcaaagccaa tctcgaaggc attcaagcca ctattacgat gcagcagtcg ataattcaaa
     4861 gtgccagtga aacatggcag gcagtaaaac atgaggagca aaagcgcctg cgagatactg
     4921 aacgttatga aaaactcgca caatcagcgg caatatcgca acaaattatt gataacgcca
     4981 ggtttgacta ccagcaggtc gcagcaaagg agcgcaaagc cgcgaacgat ttcctggtgg
     5041 agaagcaacg gctggcagtt ttatctgctc aggaagaaaa cgtacgtgcg agtatcgaag
     5101 aagttcaggc cgctttgact caggcactac tggatctgga atataccctt gt
//
LOCUS       DAEEUE010000187         5023 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.187, whole genome shotgun sequence.
ACCESSION   DAEEUE010000187 DAEEUE010000000
VERSION     DAEEUE010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.187"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            47..121
                     /gene="tnaC"
                     /locus_tag="KLK84_003977"
     CDS             47..121
                     /gene="tnaC"
                     /locus_tag="KLK84_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HBE2011101.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            342..1757
                     /gene="tnaA"
                     /locus_tag="KLK84_003978"
     CDS             342..1757
                     /gene="tnaA"
                     /locus_tag="KLK84_003978"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HBE2011102.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYRKEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1848..3095
                     /gene="tnaB"
                     /locus_tag="KLK84_003979"
     CDS             1848..3095
                     /gene="tnaB"
                     /locus_tag="KLK84_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HBE2011103.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3227..4402
                     /gene="mdtL"
                     /locus_tag="KLK84_003980"
     CDS             3227..4402
                     /gene="mdtL"
                     /locus_tag="KLK84_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HBE2011104.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGIMLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4377..>5023
                     /gene="yidZ"
                     /locus_tag="KLK84_003981"
     CDS             4377..>5023
                     /gene="yidZ"
                     /locus_tag="KLK84_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HBE2011105.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
BASE COUNT         1145 a         1208 c         1255 g         1415 t
ORIGIN      
        1 ctgagtgtaa taatgtagcg gcgtgtcttt cgaggataag tgcattatga atatcttaca
       61 tgtatgtgtg acctcaaaat ggttcaatat cgacaataag attgtcgatc accgccctta
      121 atttaccctt ctgtagccat caccagagcc aaaccgatta gattcaatgt gatcgattag
      181 tttgctatat cttaattttg ccttttgcaa aggccatctc tcgtttattt acttatttta
      241 gtaaatgatg gtgcttgtat atatacatgg tgaattaatg gatattgcag atgtaatatt
      301 cacagggatc actgtaatta aaataaatga aggattatgt aatggaaaac tttaaacatc
      361 tccctgaacc gttccgcatt cgtgttattg agccagtaaa acgtactact cgcgcttatc
      421 gtaaagaagc aattattaaa tccggtatga acccgttcct gctggatagc gaagatgtgt
      481 ttatcgattt actgaccgac agcggcaccg gggcggtaac ccaaagtatg caggcagcga
      541 tgatgcgcgg cgacgaagcc tacagcggca gccgcagcta ctatgcgtta gccgagtcag
      601 tgaaaaatat ctttggttat caatatacta ttccgactca ccagggccgt ggcgcagagc
      661 aaatctatat tccggtactg attaaaaaac gcgagcagga aaaaggcctg gatcgcagca
      721 aaatggtggc attctctaac tatttctttg ataccacgca gggccatagc cagattaacg
      781 gctgtaccgt gcgtaacgtc tatatcaaag aagccttcga tacgggcgtg cgttacgact
      841 ttaaaggcaa ctttgacctc gaaggattag aacgcggtat tgaagaagtt ggcccgaata
      901 acgtgccgta tatcgttgca accatcacca gtaactctgc aggtggtcag ccggtttcac
      961 tggcaaactt aaaagcgatg tacagcatcg cgaagaaata cgatattccg gtggtaatgg
     1021 actccgcacg ctttgctgaa aacgcctatt tcatcaagca gcgtgaagca gaatacaaag
     1081 actggaccat cgagcagatc acccgcgaaa cctacaaata tgccgatatg ctggcgatgt
     1141 ccgccaagaa agatgcgatg gtgccgatgg gcggcttgct gtgcatgaaa gacgacagct
     1201 tctttgatgt gtacaccgag tgcagaaccc tttgcgtggt gcaggaaggc ttcccgacat
     1261 atggcggcct ggaaggcggc gcgatggagc gtctggcggt aggtctgtat gacggcatga
     1321 atctcgactg gctggcttat cgtatcgcgc aggtacagta tctggtcgat ggtctggaag
     1381 agattggcgt tgtctgccag caggcgggcg gtcacgcggc attcgttgat gccggtaaac
     1441 tgctgccgca tatcccggca gaccagttcc cggcacaggc gctggcgtgc gagctgtata
     1501 aagtcgccgg tatccgtgcg gtagaaattg gctctttcct gttaggccgc gatccgaaaa
     1561 ccggtaaaca actgccatgc ccggctgaac tgctgcgttt aaccattccg cgcgcaacat
     1621 atactcaaac acatatggac ttcattattg aagcctttaa acatgtaaaa gagaacgcgg
     1681 cgaatattaa aggattaacc tttacctacg aaccaaaagt attgcgtcac ttcaccgcaa
     1741 aactgaaaga agtttaatta atactacaga gtggctataa ggatgttagc cactctctta
     1801 ccctacatcc tcaataacaa aaatagcctt cctctaaagg tggcatcatg actgatcaag
     1861 ctgaaaaaaa gcactctgca ttttggggtg ttatggttat agcaggtaca gtaattggtg
     1921 gaggtatgtt tgctttacct gttgatcttg ccggtgcctg gtttttctgg ggtgccttta
     1981 tccttatcat tgcctggttt tcaatgcttc attccgggtt attgttatta gaagcaaatt
     2041 taaattatcc tgtcggctcc agttttaaca ccatcaccaa agatttaata ggtaacacct
     2101 ggaacattat cagcggtatt accgttgcct tcgttctcta tatcctcact tatgcctata
     2161 tctctgctaa tggtgcgatc attagtgaaa cgatatcaat gaatttgggc tatcacgcta
     2221 atccacgtat tgtcgggatc tgcacagcca ttttcgttgc cagcgtattg tggataagtt
     2281 cgttagccgc cagtcgcatt acctcattgt tcctcgggct gaagattatc tcctttgtga
     2341 tcgtgtttgg ttctttcttc ttccaggtcg attactccat cctgcgcgat gccaccagca
     2401 ccactgcggg aacgtcttac ttcccgtata tctttatggc tttgccggtg tgtctggcgt
     2461 catttggttt ccacggcaat attcccagcc tgattatttg ctatggcaaa cgcaaagata
     2521 agttaatcaa aagcgtggta tttggttcgc tgctggcgct ggtgatttat ctcttctggc
     2581 tctattgcac gatggggaat attccgcgcg aaagctttaa ggcgataatc tcctcaggcg
     2641 gcaacattga ttcgctggtg aaatcgttcc tcggcaccaa acagcacggc attatcgagt
     2701 tttgcctgct ggtgttctct aacttagctg ttgccagttc gttctttggt gtcacgctgg
     2761 ggttgttcga ttatctggcg gacctgttta agattgataa ctcccacggc gggcgtttca
     2821 aaaccgtgct gttaaccttc ctgccacctg cgttgttgta tctgatcttc ccgaacggct
     2881 ttatttacgg gatcggcggt gccgggctgt gcgccaccat ctgggcggtc attattcccg
     2941 cagtgcttgc aatcaaagct cgcaagaagt ttcccaatca gatgttcacg gtctggggcg
     3001 gcaatcttat tccggcgatt gtcattctct ttggtataac cgtgattttg tgctggttcg
     3061 gcaacgtctt taacgtgtta cctaaatttg gctaaatcct tcaagaagcc agccattcgc
     3121 tggcttcttg cctctcagga aatcacttat gtccaaatgg caactcgcct tatcctcctt
     3181 caccacgtat gctttgcgtc accttactat caggacgctt tagcccatgt cccgcttttt
     3241 gatttgtagt tttgccctgg ttttacttta tcccgccggg attgatatgt acctcgttgg
     3301 tttaccgcgc atcgccgccg atctcaatgc cagcgaagcg cagttgcata ttgcgttctc
     3361 cgtatatctg gcggggatgg cagctgcgat gctatttgcc ggtaaagtgg ccgatcgttc
     3421 agggagaaag ccggtcgcca tacccggcgc ggcgctattt attattgcct cggtgttctg
     3481 ttcactggct gaaaccagca cgttatttct tgcaggccga tttctacagg ggttgggcgc
     3541 aggctgttgt tacgtggtgg cgttcgccat tttgcgcgac acgctggatg atcgacgtcg
     3601 ggctaaagtg ctgtcattac tcaacggtat tacctgcatc attccggtgt tagcgccagt
     3661 gctcggacat ctgattatgc ttaaattccc gtggcagagt ctgttctggg cgatggcaat
     3721 gatgggcatc gcggtactga tgttgtcttt gtttatttta aaagaaacgc gcccagcgtc
     3781 ccccgctgct tcggacaaac cacgagaaaa tagcgagtcg ctgcttaatc gttttttcct
     3841 cagccgtgtt gttatcacca ccctcagcgt ttcggtgatc ctcactttcg tcaatacatc
     3901 gccggtattg ctgatggaaa tcatggggtt tgagcgcgga gaatacgcca ccattatggc
     3961 gctgaccgct ggcgtcagca tgaccttttc attctcgacg ccatttgcgc tgggaatttt
     4021 taagccacgt acgttgatga tcacctcgca ggtgttattc cttgcggcgg ggatcaccct
     4081 tgccgtttca ccttcccatg cggtttctct gtttggtatc atgctgattt gcgccggttt
     4141 ctcggtaggt tttggcgtag cgatgagtca ggcgttagga ccattttcat tacgcgcggg
     4201 cgtagccagc tcgaccttag gtattgcgca ggtttgcggt tcgtcactgt ggatttggct
     4261 ggccgcggtg gttggtatcg gcgcatggaa tatgctgatc gggattctga ttgcctgtag
     4321 catagtgagc ctgttgctga ttatgttcgt cgcgcctgga cgccccgttg ccgctcatga
     4381 agaaatccat caccacgctt gatctcaatc tgctgctctg tctgcaactg ctgatgcagg
     4441 agcgcagcgt gaccaaagcg gcgaagcgga tgaacgtgac cccttcagcg gtgagtaagt
     4501 cgctggcaaa gttaagagcg tggtttgacg acccgctctt tgtgaactca ccgctggggc
     4561 tgtcgcccac acagctaatg gtcagcatgg agcaaaatct ggcggaatgg atgcaaatga
     4621 gcaatctgct gctggataaa ccgcaccacc agacaccgcg cggcctgaag tttgagttgg
     4681 cggcggaatc gccgctgatg atgatcatgc ttaatgcgct gtcgaaacgg atctaccaac
     4741 gttacccgca ggcgaccatc aaactacgta actgggatta cgattcctta gatgccatta
     4801 ctcgtggtga agtggatatt ggtttttccg gtcgcgaaag ccatcctcgc tcgcgggagc
     4861 tgttaagctc gctaccgtta gccattgatt atgaagtgct gtttagtgat gtaccctgcg
     4921 tctggttacg caaagatcat ccggcactgc atgaaacgtg gaatctggac acctttttac
     4981 gttatccgca tatcagcatt tgctgggaac agagcgacac ctg
//
LOCUS       DAEEUE010000188         4973 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.188, whole genome shotgun sequence.
ACCESSION   DAEEUE010000188 DAEEUE010000000
VERSION     DAEEUE010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.188"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(186..770)
                     /gene="kptA"
                     /locus_tag="KLK84_003982"
     CDS             complement(186..770)
                     /gene="kptA"
                     /locus_tag="KLK84_003982"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HBE2011106.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="KLK84_003983"
     CDS             1020..1703
                     /locus_tag="KLK84_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HBE2011107.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="KLK84_003984"
     CDS             1700..2161
                     /locus_tag="KLK84_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBE2011108.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLSGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="KLK84_003985"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="KLK84_003985"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBE2011109.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="KLK84_003986"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="KLK84_003986"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HBE2011110.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGGDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="KLK84_003987"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="KLK84_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HBE2011111.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="KLK84_003988"
     CDS             complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="KLK84_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HBE2011112.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         1175 a         1303 c         1240 g         1255 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagtc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag
      961 ttccaggaat tttcctggct caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct tttttcccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg tggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt atccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcggcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggt gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtgag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaaa cgctagtata ttctgaatat tccgaatagt tac
//
LOCUS       DAEEUE010000189         4878 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.189, whole genome shotgun sequence.
ACCESSION   DAEEUE010000189 DAEEUE010000000
VERSION     DAEEUE010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.189"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..128
                     /locus_tag="KLK84_003989"
     CDS             <1..128
                     /locus_tag="KLK84_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBE2011113.1"
                     /translation="TAYATIKGIEVMRALRKGQASAFYYGDPLGEMRLVSRVFEM"
     gene            255..1115
                     /locus_tag="KLK84_003990"
     CDS             255..1115
                     /locus_tag="KLK84_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HBE2011114.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            1325..1729
                     /locus_tag="KLK84_003991"
                     /pseudo
     CDS             1325..1729
                     /locus_tag="KLK84_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(1836..2672)
                     /locus_tag="KLK84_003992"
     CDS             complement(1836..2672)
                     /locus_tag="KLK84_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HBE2011115.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(2672..3475)
                     /locus_tag="KLK84_003993"
                     /note="aph(3'')-Ib"
     CDS             complement(2672..3475)
                     /locus_tag="KLK84_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HBE2011116.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(3536..4351)
                     /gene="sul2"
                     /locus_tag="KLK84_003994"
     CDS             complement(3536..4351)
                     /gene="sul2"
                     /locus_tag="KLK84_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HBE2011117.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            4681..4857
                     /locus_tag="KLK84_003995"
     CDS             4681..4857
                     /locus_tag="KLK84_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HBE2011118.1"
                     /translation="MLTDTKLRNLKPRDKLYKVNDREGLYVGVASENGIYGHSRFCNT
                     DFDDKLACLNLSGV"
BASE COUNT         1201 a         1276 c         1315 g         1086 t
ORIGIN      
        1 agacggctta cgccaccatc aaaggtattg aggtgatgcg tgcactacgc aaaggccagg
       61 cctcagcatt ttattatggt gatcccctgg gcgaaatgcg cctggtaagc agagtttttg
      121 aaatgtaagg cctttgaata agacaaaagg ctgcctcatc gctaactttg caacagtgcc
      181 tacattcaaa tatgtatccg ctcatgagac aataaccctg gtaaatgctt caataatatt
      241 gaaaaaggaa gagtatgagt attcaacatt ttcgtgtcgc ccttattccc ttttttgcgg
      301 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
      361 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
      421 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
      481 gtgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
      541 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
      601 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgct gccaacttac
      661 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
      721 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
      781 gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac
      841 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
      901 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
      961 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     1021 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     1081 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     1141 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     1201 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     1261 cctaaaagaa ctttccgcta agcgatagac tgtatgtaaa cacagtattg caaggacgcg
     1321 gaacatgcct catgtggcgg ccaggacggc cagccgggat cgggatactg gtcgttacca
     1381 gagccaccga cccgagcaaa cccttctcta tcagatcgtt gacgagtatt acccggcatt
     1441 cgctgcgctt atggcagagc agggaaagga attgccgggc tatgtgcaac gggaatttga
     1501 agaatttctc caatgcgggc ggctggagca tggctttcta cgggttcgct gcgagtcttg
     1561 ccacgccgag cacctggtcg ctttcagctg taagcgtcgc ggtttctgcc cgagctgtgg
     1621 ggcgcggcgg atggccgaaa gtgccgcctt gctggttgat gaagtactgc ctgaacaacc
     1681 catgcgtcag tgggtgttga gcttcccgtt tcagctgcgt ttcctgtttg gggtcgtttg
     1741 cgggaagggg cggaatccta cgctaaggct ttggccagcg atattctccg gtgagattga
     1801 tgtgttccca ggggatagga gaagtcgctt gatatctagt atgacgtctg tcgcacctgc
     1861 ttgatcgcgg ccgcgatagc tagatcgcgt tgctcctctt ctccatccgc gttccaagct
     1921 gcggaaaggc acccataagc gtacgcctgg tcgagcaggc gacgcggatc gacgtccagc
     1981 gcacgagaga atgcgtccgc catctgtgca atgcgtctag gatcgagaca aaggtcgtct
     2041 ctgtcagccg gatcgtagaa catattggcg gcgccaaagc ccacttcacc gaccagaccg
     2101 acgggatcta tcaccagcca gccgcgactg gagaacatga tgttttcatg atgcagatcg
     2161 ccatgtagcc cacgcagttc cgaggcattg ctcatcattt gatcggctat aatcgccgcg
     2221 tggacgtagt cagtttgaca acctgcgttt tgatcatcgc gcgcccgctg aaacaaagct
     2281 gcaaagcgat cccggatcgg gagaagggca gaaggcaggg gttcctcaga tgcggcatac
     2341 agcttcgcca ttagttccgc tgcaatttcg gtcgcctggt agtcgccgtg ctcggcaacg
     2401 atgtgagaga gcattcgctc cccggcatat tcgagcaaca tcagattgtt ctcacgaccg
     2461 agcaaccgga ctgctcccct cccattgcgc cataccagat agtcggcccc gcgcagttca
     2521 tcagcaatgt cttctatagg tttcaatccc ttgacgattg caggagtccc gtctggcaat
     2581 gaaactttcc aaacgaggct ggaaaaggtg tccgcaatga gaacaggttg cgaaacgtgc
     2641 caatgagcag gaaaaacagg cggcatgaac atcaacccca agtcagaggg tccaatcgca
     2701 gatagaaggc aaggcgttcg cggtcggggg cttcgatccc caatacattg aataggacag
     2761 cgaaggcgcg ctctgcttca tctggcgctg cccagttctc ttcggcgtta gcaatcatga
     2821 gtgccaaatc ggcatagcga tctgctgttc cgagccgccc aaggtcgatc agacccgtgc
     2881 attgaagagt tttagggtcc accatgaagt tcggcatgca gggatcacca tggcaaacaa
     2941 ccatatcggt gcgctcttgg tcgagccgca ccggtagctc tcgttcgaca cgagccaaaa
     3001 gatcgagctg cggcgtactc ttgtcctcgt ccggtaagaa gtcgggattg acggcattgc
     3061 gggacaccac atcaacggcg cgtccgaaca ttcgcgacag cctgcgctca aacggacatt
     3121 gatcaaccga taggctgtga acagcgccaa gttgctgccc cattgacggc cacgctttga
     3181 gcaaatccgc tccagacaga tcagccgccg gtactcccgg aattgccgtt atcaccaagc
     3241 atgcaccctc ctgttcctcc tgccagttga tgacctcggg gcaagccaca cctcgacctt
     3301 tgagccaaat gaggcggtca cgctctccag cgagctcacc gcggcgggaa gcaggtgcga
     3361 ttttcgcgaa ggcatgcccg tcaccacgtc gaaaaacaaa atcaccagat tctccgcctc
     3421 tgacaggcaa ccagtcagaa tgcgattcac caaaaaaaat attagttcga ttcaatggag
     3481 gttccttcag ttttctgatg aagcgcgaat atagagaaat atcccgaatg tgcagttaac
     3541 gaattcttgc ggtttctttc agcgccgcca ataccgccag cccgtcgcgc aaggggcgcg
     3601 gctcgtgtgt gcggatgaag tcagctccac ctgcggcggc ggcaagctct gcagcgagtg
     3661 tcgcggcccc gacatccccc ggaccacggc ctgtgagcgc gcgcagaaag gatttgcgcg
     3721 aaacagacag aagcaccggc aaatcgaagc gcagccgcaa ttcatcgaac cgcgccagca
     3781 ccgagagcga ggtttcggga gcagccccca gaaaaaaccc catgccggga tcaaggacaa
     3841 ggcggttgcg tttgataccg gcacccgtca gcgccgcgat gcgcgcgtca aagaacgccg
     3901 caatgtgatc catgatgtcg ccagcgggtg cctcgcgccg atctgcctgc ccgtcttgca
     3961 ccgaatgcat aacgacgagt ttggcagatg atttcgccaa ttgcggatag aacgcagcgt
     4021 ctggaaaacc gcgaatatca ttgagatagg ccacaccacg cgacaaggca taggcttgcg
     4081 tcgcgggttg ataactgtcg agcgagacgg gaatgccatc tgccttgagc gcgtccagca
     4141 ccggcgcgat acgcgcgatt tctgtgtcgg acgaaacagg cgcggcgtcg ggattgctgg
     4201 atgccggacc gaggtcgatc acatctgccc cctcggccat cagcttacgc gcctgcgcaa
     4261 tggctgcgtc tggcgccaga taccggcctc catcggagaa actgtccgag gttatgttga
     4321 cgatgccgaa aatgatgagc gatttattca tgggggcttc tataataata ataatcgagc
     4381 atgagtctca tacggatgct cgggtcgaaa gggaatcccc aggcgagtaa cctgtttgcg
     4441 gtgctccatt agctgcagga gcagaatagc atacatctgg aagcaaagcc aggaaagcgg
     4501 cctatggagc tgtgcggcag cgctcagtag gcaatttttc aaaatattgt taagcctttt
     4561 ctgagcatgg tatttttcat ggtattacca attagcagga aaataagcca ttgaatataa
     4621 aagataaaaa tgtcttgttt acaatagagt gggtatcgga gacgaagaag gacaaggtac
     4681 atgctgaccg ataccaagct gcgcaatctc aagcccaggg acaaactcta caaagtgaat
     4741 gaccgggaag gtctctatgt gggggtcgcc tcagaaaacg gaatctatgg tcactcccgt
     4801 ttttgcaaca ccgattttga cgacaagttg gcttgcttga atctatccgg cgtctgaatg
     4861 ggattttatt cccgcgcc
//
LOCUS       DAEEUE010000190         4873 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.190, whole genome shotgun sequence.
ACCESSION   DAEEUE010000190 DAEEUE010000000
VERSION     DAEEUE010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.190"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..529
                     /locus_tag="KLK84_003996"
     CDS             <1..529
                     /locus_tag="KLK84_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBE2011119.1"
                     /translation="RTTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSIT
                     LLNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSV
                     IFINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLK
                     HDAVLIEAMESRSLR"
     gene            726..2165
                     /gene="aldA"
                     /locus_tag="KLK84_003997"
     CDS             726..2165
                     /gene="aldA"
                     /locus_tag="KLK84_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBE2011120.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2207..3208)
                     /gene="gap"
                     /locus_tag="KLK84_003998"
     CDS             complement(2207..3208)
                     /gene="gap"
                     /locus_tag="KLK84_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBE2011121.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     ADKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            3397..3927
                     /gene="cybB"
                     /locus_tag="KLK84_003999"
     CDS             3397..3927
                     /gene="cybB"
                     /locus_tag="KLK84_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HBE2011122.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4172..4345
                     /locus_tag="KLK84_004000"
     CDS             4172..4345
                     /locus_tag="KLK84_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011123.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(4417..4566)
                     /gene="hokB"
                     /locus_tag="KLK84_004001"
     CDS             complement(4417..4566)
                     /gene="hokB"
                     /locus_tag="KLK84_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HBE2011124.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(4667..4807)
                     /locus_tag="KLK84_004002"
                     /pseudo
     CDS             complement(4667..4807)
                     /locus_tag="KLK84_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1247 a         1122 c         1208 g         1296 t
ORIGIN      
        1 ccgcaccact ggcagagcag aagcgaccat cctggtggat atcgctcatc agttctggca
       61 tattccgcat gaaaaaatct ggattgaaga ccagtcaaca aactgcggtg aaaacgcacg
      121 ctttagcatc acgctattga atcaggccgt agaacgagtt catacggcta tcgttgtaca
      181 ggaccccacc atgcagcggc gcacgatggc aacgttccgc cgtatgaccg gggacaatcc
      241 cgatgcacca cgctggttaa gttatcccgg attcgttcct cagttaggaa ataacgcaga
      301 cagtgtaatc tttattaatc agttacaagg attatggccg gttgagcgtt atctctcact
      361 actcactggc gagctgccgc gtttacacga tgatagcgat ggctacggtc cccgcgggcg
      421 agattttatc gttcacgttg attttccggc agaagtcatc catgcatggc aaacgctgaa
      481 acatgatgcg gtgctcatcg aggcgatgga aagtcgctcg ttacgttaaa aattgcccgt
      541 ttgtgaacca cttgtttgca aacgggcatg actcctgact tttatttctg ccttttattc
      601 cttttacact tgtttttatg aagccctcca cagacttgtc ctttcccgat gccgtctctc
      661 tgatgattga tgttaattaa caatatattc accgaaaaca aacatataaa tcacaagagt
      721 cgcccatgtc agtacccgtt caacatccta tgtatatcga tggacagttt gttacctggc
      781 gtggagacgc atggattgat gtggtaaacc ctgctacaga ggctgtcatt tcccgcatac
      841 ccgatggtca ggccgaggat gcccgtaagg caatcgatgc agcagaacgt gcacaaccag
      901 aatgggaagc gttgcctgct attgaacgcg ccagttggtt gtgcaaaatc tccgccggga
      961 tccgcgaacg cgccagtgaa atcagtgcgc tgattgttga agaagggggc aagatccagc
     1021 agctagctga agtcgaagtg gcttttactg ccgactatat cgattacatg gcggagtggg
     1081 cacggcgtta cgagggcgag attattcaaa gcgatcgtcc gggagaaaat attcttctgt
     1141 ttaaacgtgc gcttggtgtg actaccggca ttctgccgtg gaactttccg ttcttcctca
     1201 ttgcccgcaa aatggctccc gctcttttga ccggtaatac catcgtcatt aaacccagtg
     1261 aatttacgcc aaacaatgcg attgcattcg ccaaaatcgt cgatgaaata ggccttccgc
     1321 gcggcgtgtt taaccttgta ctggggcgtg gtgaaaccgt tgggcaagaa ctggcgggta
     1381 acccaaaggt cgcaatggtc agtatgacag gcagcgtctc tgcaggtgag aagatcatgg
     1441 cgactgcggc gaaaaacatc accaaagtgt gtctggaatt gggtggtaaa gcaccggcta
     1501 tcgtaatgga cgatgccgat cttgaactgg cagtcaaagc catcgttgat tcacgcgtca
     1561 ttaatagtgg gcaagtgtgt aactgtgcag aacgtgttta tgtacagaaa ggcatttatg
     1621 atcagttcgt caatctgctg ggtgaagcgt tgcaggcggt tcaatttggt aaccccgctg
     1681 aacgcaacga cattgcgatg gggccgttga ttaacgccgc ggcgctggaa agggttgagc
     1741 aaaaagtggc gcgcgcagta gaagaagggg cgagagtggc gttgggtggc aaagcggtag
     1801 aggggaaagg atattattat ccgccgacat tgctgctgga tgttcgccag gaaatgtcga
     1861 ttatgcatga ggaaaccttt ggcccggtgc tgccagttgt cgcatttgac acgctggaag
     1921 atgctatctc aatggctaat gacagtgatt acggcctgac ctcatcaatc tatacccaaa
     1981 atctgaacgt cgcgatgaaa gccattaaag ggctgaagtt tggtgaaact tacatcaacc
     2041 gtgaaaactt cgaagctatg caaggtttcc acgccggatg gcgtaaatcc ggtattggcg
     2101 gcgcagatgg taaacatggc ctgcatgaat atctgcagac ccaggtggtt tatttacagt
     2161 cttaatgagt gaaagaggcg gaggtttttt cctccgcctg tgcgcgtcag agtttagcga
     2221 atttttcgag ggtgcgaata agctgcgtga cgaagccata ttcgttatcg taccaggcga
     2281 ccgttttcac cagttgtaaa tcgcccacgg cggtaatttc cgtttgcgtg gcatcaaaca
     2341 ctgaaccgaa atggctgcca atgatatcgg aagagactat ttcttcatcg gtataaccaa
     2401 atgactcgtt attggtggtt gcttgtttaa gtgcgttatt cacctcttcg gcagtcactt
     2461 tttttccgag aatggacacc agctcagtga ccgaacctgt tttcaccggc acgcgttgcg
     2521 catgaccttt cagtttgccg ctcagttccg ggatcaccag accaatggct tttgccgccc
     2581 ccgtagtgtg gggaatgata ttttctgccg ctgcgcgtga agcacgtaaa tctttaccac
     2641 gcgggccatc caccagtgac tgggtgccag tataggcatg aatggtcgtc atcgtgccga
     2701 cttctatccc gaaactgtca tgcaaggctt tggccatcgg cgcaaggcag ttagtggtgc
     2761 atgacgccac ggaaacaatg gtgtcgttgc catccagagt gtcgtcatta acgttataaa
     2821 cgatggtttt catttcaccg gcaggagcgg aaatcaacac cttcttcgca ccagcatcaa
     2881 gatgcgcctg cgatttatcg gcggaggtat aaaagccagt acattcgaca atgatttctg
     2941 cccctttcgc tttccacgga atatttttag cctctttttc ggcgtaaacc gcgatacttt
     3001 tcccatcaac gataagtgaa tcttccgtaa aatcaacgct ccaggggaac ggaccatagt
     3061 ttgaatcatg tttcagcaga taggcgagaa tttttgggga agtaagatca ttaatagcga
     3121 caacgtctat gttgcttttg acttcaagta aacgacgcaa caccagtcga ccgatacgac
     3181 caaaaccgtt aataccaact ttactcatgg ttttctcctg tcaggaacgt tcggatgaaa
     3241 attgatcctt tccaggctta gaccaggatt gtgggatggg caatctccat tcttacagtg
     3301 aaacgtaacg tactgaaaac gggtgaacaa tatttaatga aattttgaga aaagcccgtt
     3361 atgttaacgg aaaattatgt taaagcagga aatgttatgg aaaataaata ctcaaggtta
     3421 caaatcagca ttcactggct ggtctttttg ctggttatcg cagcgtattg cgcaatggag
     3481 tttcgtggtt tcttcccacg tagcgatcgg ccgctcatca acatgattca tgtttcctgt
     3541 ggcatctcaa ttctcgtgct gatggtggtt cgtcttttgt taagactgaa atacccaacc
     3601 ccgccgatta tacctaagcc gaaaccgatg atgacgggac tggcgcattt gggacatttg
     3661 gtgatttatc tcctgtttat tgcgctgcca gtgattggtt tggtgatgat gtataaccgg
     3721 ggcaacccgt ggtttgcgtt tggtttgacg atgccttacg cttcagaggc caatttcgaa
     3781 cgggttgata gcttaaagtc gtggcatgag acgctggcga atctgggata ttttgtcatc
     3841 gggttgcacg ctgcggctgc actggcacac cactatttct ggaaggacaa cacacttcta
     3901 cgcatgatgc cgcgtaaacg ttcctgaagg atatttaaag aaaacgcctg tactaaaacc
     3961 gacccgtggt acaggcgaag aatacgggtc tacatcggaa gcgcctatta tatttatttg
     4021 tatgacaaat aaaaccataa tccttgccca tacgtccatc tggcttattt ttaatcaatt
     4081 cacccgatct ttgatctcat caacggtatc aaaataaacc agcgtataac gttcaaatcg
     4141 cacataagat tcgataaacc atggaggtta tatgaaaaaa ctggcactta ttttgtttat
     4201 gggaacgctt gtttcctttt atgccgatgc cgggcgcaaa ccctgttctg gttcgaaagg
     4261 ggggatctca cactgtacgg caggcggcaa atttgtctgt aatgatggtt ctattagtgc
     4321 atcgaaaaaa acatgcacta actgaagtgt aaaaggggtg ccatgagaaa atgaatctgc
     4381 tagtcagatg cgcggggaaa atccccgcgc ttgcccttac ctggacgtgc aggccatgag
     4441 cgcagcaacc tccttatcac cgtcccggaa ccgcagttcg tagagcgttt gtcgggtcag
     4501 gagtgtgaat gccagaatcg taatgcagat aatgagcaga cacaccacca gagggttgcg
     4561 cttcatagcc tttctccttg ccggatggcg ggtaagaggc taacaatctg aattgcttgg
     4621 ttcattcgtt ggcctcggtt tgtactgacc ccaaaaagtt ggacagttaa acacgaggca
     4681 tataggtctg attccgatat tcatttggag tcagaccttt taatttcagg ctaattcttc
     4741 tgctgttgta gtattcaata tattccgtaa cagcatcctt cagttcgctt atattactga
     4801 actcattaga ctggccccct gaatctccag acaaccagta tcacttattt aagtgatagt
     4861 cttaatacta gtt
//
LOCUS       DAEEUE010000191         4867 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.191, whole genome shotgun sequence.
ACCESSION   DAEEUE010000191 DAEEUE010000000
VERSION     DAEEUE010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.191"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..970
                     /locus_tag="KLK84_004003"
     CDS             <1..970
                     /locus_tag="KLK84_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBE2011125.1"
                     /translation="AEMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGR
                     GYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTF
                     GWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIK
                     VSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFI
                     YGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMAL
                     IIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            1551..2774
                     /locus_tag="KLK84_004004"
     CDS             1551..2774
                     /locus_tag="KLK84_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011126.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIAFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            3021..3587
                     /gene="slp"
                     /locus_tag="KLK84_004005"
     CDS             3021..3587
                     /gene="slp"
                     /locus_tag="KLK84_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HBE2011127.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            3743..4273
                     /locus_tag="KLK84_004006"
     CDS             3743..4273
                     /locus_tag="KLK84_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE2011128.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(4315..4867)
                     /locus_tag="KLK84_004007"
                     /pseudo
     CDS             complement(4315..4867)
                     /locus_tag="KLK84_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="MgtC/SapB family protein"
BASE COUNT         1556 a          999 c          905 g         1407 t
ORIGIN      
        1 cgcagagatg tttgtgtttc tcgccgtcga gctttcgttg ctgtttatag tgattagtgc
       61 cggtgtcagc ctgataagac aaaaggtgcc agaccataaa atccagcaga tgatgggggc
      121 cagaaaaggg agaggctatc tcctggctgc tctgttggga gccgttaccc cgttctgtag
      181 ttgctcgaca atacccatgt tacgtggatt gttgtcagcg aaagccgggt ttggtccgac
      241 tctcactttt ttatttgttt caccattact taatcccatt atcgtcggac taatgtggat
      301 gacctttggt tggaaagtca ccttgttgta cgcgattatc gcagccggcg tctccgttct
      361 tgccagtatt atcctggatt ctctggggtt tgaacgtcat atcattgcca gtaaaagctc
      421 atcagcaaat tgttgtgctc cagcaaaaac ttcgccggga acgacatata cgcccataaa
      481 agtgagttgt tgtagtccag cggctaaagc cattgagaac cccgtagtta cctgttgcag
      541 taccaaagct gtagtaagta ttaatcccat aaaactagcc accaaagatg cgttgcaaca
      601 gtttaaagat gtactgccat atcttttgtt aggagtatta ataggatctt ttatttatgg
      661 ctttattccc tcagcgtgga ttgccgctca tgcaggagca gataatccct tcgccatccc
      721 attaagcgcc gttgtcggta ttccgctgta tatccgggct gaggcggtta tccctctggc
      781 atctgttctg atgacaaaag gaatgggtct gggagcatta atggcattaa tcatcggcag
      841 tgccggcgca agcctgacgg aagtgatatt acttaaatca atgttcagaa tgccgatgat
      901 agctgcattc ctgacggtta tattaggtat ggctatcttg atgggctatt tgactcaatt
      961 gctattttaa atagatgcca gcagtaaact ataagatgca tagttagtgc catatccttc
     1021 taagtagaaa atcatagaca ggataatcct taaaaatcac aaatgattta actaatgcat
     1081 tgagaatgat gacggctcgg tcaacaagtc atcaacagac aataaccacg taatcatgct
     1141 tctctgacat tttatcggag aagcaatgac tctttgtctc agtaccctcc tcctttttaa
     1201 aaggcagatt tcagtcgcta atacaacaca tttttaaata cactttaacc tggttcatta
     1261 ttacacatct aaaacatcac aaaaatatta cttcacacac tatcggagaa cataataact
     1321 ttttaaaatg agcgtttttt gatagttgtt aacgttttcc tttacgatac tatcactgcc
     1381 ctttttttat acacaaatat taccgggtgg aaatgaaagg gaatttaaag gtaattatat
     1441 caggaagata taacgcatta tatttattgt gtgtagaaga aaatccattc ctgttctaat
     1501 tatccaattt aaaaacatta gcataaaaca gatgatgaat aaggaatgtt atgtcaattg
     1561 actttacccc aggtataata aatacatatc acggcgatat ttataactgc acaacaaata
     1621 ccgataatgt taaaacgcca gacacaccga agtggccttg tgataattgg gaagaacaac
     1681 aacccattaa ttccacattc tctggagaag gatacaaccc tgaacaattt gatttagcgc
     1741 aacaacaact ccaacaaatt aatgcatgcc acacgaacac cacatataca aatgcagact
     1801 actcaaaagt tgtggttcaa cttgtaagtc ttattaataa cattgaaacg ataagttcga
     1861 caccacttac gcaacagaca caatcaatac ttaatcagat tcataacatt agatacgaaa
     1921 aggataagga ttctgtatgt caaatcattg ttgtcgttaa tccagaaccc aacaatccaa
     1981 tcataactaa aattctcgta gaagaaggaa tacccgaaag attcagtgtc caaaccgttt
     2041 cttccgataa taaaaatttt gctgggcaac gagctgactt accaactgat ataagagaca
     2101 ttcaaagctt gtacttaaaa atggcgaagc tgtatattga gcacagtaaa aatgaaaata
     2161 ggatggaagc ccttgctgga tgcgacttta ttgatttcaa catgacaggg caagatatgt
     2221 ccaagctagt cctaacctta tcaaaatttt attttgaaga tctacttaat ataaatttca
     2281 ctgacgcaaa tttatttaac actatttttt cacacaagga aaatcccatc cccaaactac
     2341 ataaatatga acaacatctt gacaaacaaa tcaatggttt gttttctaca ttgttaacta
     2401 ttaatgacaa ctctttacgc gcgaaagcgg aaattgcttc gagaataatt gatttcttag
     2461 aggcaaaggt agtgaatctt tcatttgatg acatattaaa atataagcaa gagttcaaaa
     2521 aaatatgtta taaacaactt caagagttta caacaccctc tctatacaat aaaattcaaa
     2581 aatgggcaac aatgtctaaa aatgaattca tagcatttca ttacgagaca cttcaacccg
     2641 aaaagatttc ataccccttt tatttaaaga gagatctgcc taatgaaaaa gatataaatt
     2701 atggggtgga aatagaaata ccatctggca aaagaattcg cttatcaaat cattatcaga
     2761 atataatacc ataatattga ttattagcac tttataatca ttgaataaaa atagatttta
     2821 tgtaatttta aaacaaaggg ttatattatt gggcgatgga tactcatacc acgccccaaa
     2881 ataacggaat tgaactaaac agaagggata taaacatcag acaggtttac gttactatca
     2941 ggcatatcac ctcagaatca gatgaaaact ataaagaaat atctattatg gttttaatat
     3001 ttgttgataa ggatagtaac atgaacatga caaaaggtgc actcatcctc agcctttcat
     3061 ttttgcttgc cgcatgtagt tcaattccgc aaaatatcaa aggcaataac caacctgata
     3121 ttcaaaaaag ttttgttgct gttcataacc agccggggtt atatgttggt caacaagcgc
     3181 gctttggtgg gaaggttatc aacgttatca atggcaaaac ggatacgttg ttagaaatcg
     3241 ctgtattacc gttggatagc tatgcgaagc ctgatattga agccaactat cagggccgac
     3301 tgctcgccag acaaagcggc ttccttgatc cagtgaacta tcgtaatcac tttgttacca
     3361 tcctcggtac cattcagggt gaacaaccag gctttatcaa taaagtcccg tataacttcc
     3421 tggaagtgaa tatgcagggc atccaggtgt ggcatttgag agaagtggtt aataccacct
     3481 ataacctgtg ggattacggc tatggtgcat tctggccgga accgggctgg ggtgcgcctt
     3541 actacaccaa tgcggtgagt caggtaacac ctgagctggt caaataacac caccgaaaga
     3601 tgcagatgct ttctcagcat ctgcatcatg tattacataa aattaatata cagtaagcta
     3661 actattatta ttataagccc tgtcctgtta attacctttg gcaaactgat tataaagtta
     3721 atgtccgcac caggagtcgg ttatgtttct tataattacc agggatacga tgttcttcac
     3781 cgcgatgaaa aacattctga gtaaaggtaa tgtcgttcat atacagaacg aagaagagat
     3841 cgacgtaatg ttgcatcaga atgccttcgt cattattgat acattaatga ataatgtatt
     3901 tcattctaat tttctcactc aaattgaacg attaaaacct gtccatgtca ttgttttctc
     3961 cccctttaat attaaacgct gtctggggaa agtaccggtg acctttgttc cgcggactat
     4021 cactatcatt gattttgtcg cactcatcaa tggcagttac tgctctgtgc ctgaagcgaa
     4081 tgtgtcactt tcgcgcaagc aacatcaggt tctgagctgc attgcgaatc aaatgacaac
     4141 tgaagatatt ctggagaaac tgaaaatatc gctaaaaacg ttctactgcc ataaacacaa
     4201 tatcatgatg atcctcaatc ttaagcggat caatgagctg gtacgccatc agcatattga
     4261 ttatctggtg tgaatttcag gcttacggtg agtctggcta cgctgccaca cagattagct
     4321 aattgaaacg cctttcaccc ctgccatacc ttttaataat cgcaacaggt cttctatcaa
     4381 cgttgtggca tgaagctgga tatcaatggc gaccacttca tgatcttcat tttcctgcaa
     4441 cgaaactaaa tcagttttta ttttttgctg cttaaaccag tcgagcattg ataccgtatt
     4501 accgttcact aatgtcagct gtaaatggta atttttgttc atcaggcgga aggttaattg
     4561 atggaagact tccagcacca acaaggtcat cactgaacca taaataccca gttcgtacat
     4621 accgctgcca ataaccatac ctatgcggcg gtcacccaga tatccgctgc cgtcgtcaga
     4681 cctacaatat tttggttacg aacgagaatg ttacctgcac cgataaaccc gacgcccgtc
     4741 accacctgag cagcaatacg gctggggtcg agtccgacgt gatctaaaga cagcacgtca
     4801 gcaaaaccat atttcgaaac aatcataaac agggcgcttc ccatgccaat taatacatgt
     4861 gtgcgta
//
LOCUS       DAEEUE010000192         4863 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.192, whole genome shotgun sequence.
ACCESSION   DAEEUE010000192 DAEEUE010000000
VERSION     DAEEUE010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.192"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..425
                     /locus_tag="KLK84_004008"
     CDS             <1..425
                     /locus_tag="KLK84_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE2011129.1"
                     /translation="PRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLG
                     VYCSSEQKLSFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLG
                     TVNKSKVPLGLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            759..3038
                     /gene="ydeP"
                     /locus_tag="KLK84_004009"
     CDS             759..3038
                     /gene="ydeP"
                     /locus_tag="KLK84_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HBE2011130.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3286..3489
                     /gene="safA"
                     /locus_tag="KLK84_004010"
     CDS             3286..3489
                     /gene="safA"
                     /locus_tag="KLK84_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HBE2011131.1"
                     /translation="MHATTVKNKITQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3557..4318
                     /gene="ydeO"
                     /locus_tag="KLK84_004011"
     CDS             3557..4318
                     /gene="ydeO"
                     /locus_tag="KLK84_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HBE2011132.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            4720..>4863
                     /locus_tag="KLK84_004012"
     CDS             4720..>4863
                     /locus_tag="KLK84_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HBE2011133.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFT"
BASE COUNT         1390 a         1038 c         1079 g         1356 t
ORIGIN      
        1 atccgcgtaa ttttacgtgg aatattattt caaataacag tgttgttatg ccaacaggcg
       61 gctgcaccgt tgatagtcgt aatgtcaccg tggatctccc ggactttcct ggaagcgcag
      121 aaatacccct tggcgtatat tgctcaagcg aacaaaagct ctcattttat ctttcgggtg
      181 ccaccactga cagtagccga caagtgtttg ctaacacagc acctgatgca acaaaagcat
      241 cgggagtggg tgtcactcta atgcgtaatg gaaaaatact ggctacggga gaaaatgtct
      301 ccctggggac agtaaataaa agcaaagtac ctctgggttt aagcgcaacc tatggacaaa
      361 caggaaacaa ggtttcagcc ggaaccgtcc agtcagtcat tggtgtcact tttatctatg
      421 aataattgtg attttgacct tagtctatgc gctccattca cgaaaattgc gcggataatt
      481 attttgtgaa ggctattatc ctacacctgt aagaaaatcc gcgcacaatt gaagccagag
      541 tcttattttt tatattgaaa aatatgttaa tttaaacact atgagagatg atgcttaact
      601 tatcgtattt aatctattgt ttaacgataa tagattaaaa atagaaagag agtagtccct
      661 tactcttata tatccatgtt ggcgataatc cctttgtatg tactgtgcat catcgctatt
      721 acaaatccta ataattcatt tccacacagg ataagtagat gaagaaaaaa attgaatcct
      781 accagggtgc tgcaggtggt tggggtgctg ttaaatccgt agcgaatgca gtacgtaagc
      841 agatggatat acgccatgat gttattgcca tgtttgacat gaataagcca gagggctttg
      901 actgtccggg ttgtgcatgg ccagatccta agcacagtgc gtcattcgac atttgtgaaa
      961 acggcgcaaa agcaatcgcc tgggaagtca cggataagca ggtaaatgcc tctttctttg
     1021 ctgagaatac ggttcaatca ttacttacct ggggagatca cgagcttgag gctgcggggc
     1081 gtctcactca gcctttgaaa tatgatgccg tcagcgactg ttacaagcca ttaagctggc
     1141 aacaagcttt cgacgaaatt ggcgcacgcc ttcaaagcta tagtgatccc aatcaggttg
     1201 aattctatac ttcgggccgc acttccaatg aagctgcctt tctttatcag ctttttgccc
     1261 gtgaatacgg gagcaataac tttcccgact gctccaacat gtgccatgaa ccgacaagcg
     1321 tgggtttggc agcgagtatc ggtgtaggta aagggaccgt gttgctggaa gactttgaga
     1381 agtgcgattt agtcatttgc attgggcata accctggtac aaaccaccct cgcatgctga
     1441 cttcgttgcg cgctttagtg aaacggggag cgaaaatgat cgccatcaat cctctacagg
     1501 aacgtggcct ggagcgattt accgcaccgc aaaacccgtt tgaaatgctg acgaactctg
     1561 agactcagtt ggccagtgcc tactacaacg tgcgcattgg tggcgatatg gcgttgctca
     1621 aggggatgat gcgcctgtta attgagcgcg atgatgctgc aagtgccgca ggtcggccct
     1681 cattgcttga tgacgaattt attcaaacgc ataccgtcgg ctttgacgag ctacgccgtg
     1741 acgttctcaa ttccgagtgg aaagatatcg aacgtatttc tggactaagt cagacacaaa
     1801 tcgccgaact ggctgacgca tatgccgctg ccgaacgcac cattatctgt tacggaatgg
     1861 ggatcactca gcacgaacat ggtacccaga acgtgcagca actggtcaat ttgctgttga
     1921 tgaaaggtaa cattggcaag cctggtgcgg gtatctgccc actacgtggt cactctaatg
     1981 tacagggcga ccgaaccgtc ggtatcaccg agaaaccgtc tgcagagttt ctggctcgcc
     2041 tgggtgagcg ctatggcttc cccccacctc atgcacctgg acatgctgca attgccagca
     2101 tgcaagcaat atgtacgggg caggctcgag cattgatctg catggggggc aactttgcac
     2161 tggcaatgcc agatcgggaa gcgagcgctg taccgttaac gcaattagat ttggcggtac
     2221 acgtagccac taagcttaac cgctctcatc tgctgaccgc acggcatagc tatattctgc
     2281 cggttctggg gcgtagcgag attgatatgc aaaaaagcgg tgcgcaggcg gtaaccgttg
     2341 aggattcaat gtcgatgatt catgcctcgc gtggcgtgtt aaaacccgcc ggtgtaatgc
     2401 tgaaatcaga gtgtgcagtg gtcgcgggaa tcgcgcaggc agcactaccc cagagcgtgg
     2461 tagcctggga gtatctggtg gaagattatg atcgcattcg caatgacatt gaagctgtgc
     2521 tgccagagtt cgccgactat aaccagcgca tccgtcatcc cggtggtttt cacctgataa
     2581 atgcagctgc tgaaaggcgc tggatgacgc cgtcaggtaa ggctaatttc attaccagca
     2641 aagggctgtt agaagatccc tcttcagcgt ttaacagtaa gctggtcatg gcgacagtac
     2701 gcagccacga tcagtacaac acgacgattt atggtatgga tgatcgctat cgaggggtat
     2761 tcggtcaacg agatgtggtc tttatgagtg ctaaacaagc taaaatttgc cgtgtaaaaa
     2821 acggcgaaag agttaatctt attgcgctta cgccagacgg taagcgcagc tcacgccgca
     2881 tggatagatt aaaagtggtc atttacccta tggctgaccg ctcactggtg acctattttc
     2941 cagaatcgaa tcacatgcta acacttgata accacgatcc attaagtggc attcctggct
     3001 ataaaagtat tccggttgaa ttagaaccat caaattaatg cctcttctca tttcttctgc
     3061 tgtcatccgc acagcagaag aattcctcat tgactattat tgcgcaattt gctcacatgg
     3121 attaaattaa actacatact ataagatata aacttctgcc tacagctgta agaaactccg
     3181 ctcagtactg aagcaccagt cctatttctt cttttctcca gcctgttata ttaagcatac
     3241 tgattaacga cttttaacgt tatccgctaa ataaacatat ttgaaatgca tgcgaccaca
     3301 gtgaaaaaca aaatcacgca aagagataac tataaagaaa tcatgtctgc aattgtggtt
     3361 gtcttattac tgacacttac gttgatagcc attttttcgg caattgatca gctgagtatt
     3421 tcagaaatgg gtcgcattgc aagagatctt acacatttca ttatcaatag tttgcaagac
     3481 tggaaataaa cagcaaatta taaatgtgaa atgtttaaaa ggtatcgata aaactttatt
     3541 gttttaagga gataaaatgt cgctcgtttg ttctgttata tttattcatc atgctttcaa
     3601 cgctaacatt ttagataaag attacgcctt ctccgacggc gagatcctga tggtagataa
     3661 cgctgttcgt acacattttg aaccttatga gcggcatttt aaagaaatcg gatttaatga
     3721 aaataccatt aaaaaatatc tacaatgcac taacatccag acagtgacgg tgcctgttcc
     3781 tgcgaagttt ttacgtgctt caaatgtacc gactggattg cttaatgaaa tgattgctta
     3841 tctcaactcg gaagaacgca atcatcataa tttttcagaa cttttgcttt tttcttgcct
     3901 gtctattttt gccgcatgca aaggtttcat tacactatta actaacggtg tgctatccgt
     3961 ttccgggaaa gtgagaaata ttgtcaacat gaagctggcg cacccatgga agctgaaaga
     4021 tatttgtgac tgcctgtaca tcagtgaaag cctgttgaag aaaaaactaa agcaagagca
     4081 aacgacattc tcacagattc tattagatgc cagaatgcag cacgcaaaaa atttgatacg
     4141 cgtagaaggt tcagtcaata aaattgccga acaatgtggt tatgccagta catcttattt
     4201 tatttatgcg ttccgcaaac atttcggcaa cagtccgaag agagtttcta aggagtaccg
     4261 ttgtcaaagg cacacgggta tgaatacggg caacacgatg agtgctttag ctatttgatt
     4321 atttgctaac gagtagtcaa ccacgcacgc tacgtaagaa ttatatgggg cagccattcc
     4381 ctgccccgcg ttgtttttag gcgatatatt tattgaaata aataagtgac atccatcaca
     4441 tatttatgca cttacataac ctgttgcatg attatttatg atctcaattc ggcattttgt
     4501 cagtaaaatg caataattta ttaaatatca ataaattagt tgtttatcgg cgagaaatta
     4561 cttaatagaa cagaaagtaa tgtcaacgct ttatggactg ttatttccct ttttttagct
     4621 aaatctgcta tctctttatg tgactaactt cacttacatc cacttatttc tcttcgtaaa
     4681 attacttcgg aattaagtac aataagaaga ggaacattta tgaagtctgc attaaagaaa
     4741 agtgtcgtaa gtacctcgat atctttgata ctggcatctg gtatggctgc atttgctgct
     4801 catgcggcag atgatgtaaa gctgaaagca accaaaacaa acgttgcttt ctcagacttt
     4861 acg
//
LOCUS       DAEEUE010000193         4662 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.193, whole genome shotgun sequence.
ACCESSION   DAEEUE010000193 DAEEUE010000000
VERSION     DAEEUE010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.193"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            73..441
                     /locus_tag="KLK84_004013"
                     /pseudo
     CDS             73..441
                     /locus_tag="KLK84_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            703..1065
                     /locus_tag="KLK84_004014"
     CDS             703..1065
                     /locus_tag="KLK84_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBE2011134.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYTAHTSQALANFTGFVV
                     AVSFSFFANARFTFKASTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            1062..1982
                     /locus_tag="KLK84_004015"
     CDS             1062..1982
                     /locus_tag="KLK84_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBE2011135.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGAEMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPKYILKKGRK"
     gene            1985..2936
                     /locus_tag="KLK84_004016"
                     /pseudo
     CDS             1985..2936
                     /locus_tag="KLK84_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060427.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gtrI"
     gene            2963..3203
                     /locus_tag="KLK84_004017"
                     /pseudo
     CDS             2963..3203
                     /locus_tag="KLK84_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060423.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gtrI domain protein"
     gene            3200..3505
                     /locus_tag="KLK84_004018"
     CDS             3200..3505
                     /locus_tag="KLK84_004018"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011136.1"
                     /translation="MTVNWLSGGDIQITPVFFNADSGKINFTGVRQQTLASWHSKEAF
                     NSAPERQFIAVSIANEPERCKEMTSCLAGIQEQLGKPDEVLNFEGRVILVFNKKLNL"
     gene            complement(3739..4179)
                     /locus_tag="KLK84_004019"
     CDS             complement(3739..4179)
                     /locus_tag="KLK84_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HBE2011137.1"
                     /translation="MRITLQWDINEMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINSQSVTKMITCGENGHPVIVDRPSPTPEQLALINNEKKSALIAEATNVIAPLQ
                     DAVDLDMATDDETKLLLAWKKYRALLMRVDIKNTEWPKKPESNL"
     gene            complement(4151..>4662)
                     /locus_tag="KLK84_004020"
     CDS             complement(4151..>4662)
                     /locus_tag="KLK84_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011138.1"
                     /translation="CSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVK
                     TALENLGLGEAAKRNVGTGANQIPDMSLFASSNTATAAAQKFPSGLILQCGQLNGSPN
                     VSSTYGMRFPMTFSRVLAVTVTLNVTGASGQPTVSATNVQNTGFDITVSPGSGYGSSA
                     DAYYIAMGY"
BASE COUNT         1338 a          955 c          920 g         1449 t
ORIGIN      
        1 gcagccttca gggaaaaata tcatcagatg gctgcttaaa aaaagaacaa atggtagtgt
       61 ccgctattgc cagtacacct cagggtgttt gtccacacca aacctgccta ccgaagcgac
      121 ggaataaaaa cagcagcggt aaggaagatg agaaccgaca gcaacaaggc atggaaggga
      181 gcgttaaagc gggcaggcat tagcaacttc cgcttccatg acctgaggca tacctgggca
      241 agctggctgg ttcagtccgg tgtctctctt cttgcgctta aagagatggg aggatgggaa
      301 actctcgaaa tggttcaaag atacgcccac ctttcagccg ggcatctcac cgagcacgcg
      361 agcaaaatcg atgcgattat aagtcgcaat ggcacaaata cggcacaaga ggagaacgta
      421 gtttacttaa atgcgaggta acttattgat ttaaatggtg ccgataatag gagtcgaacc
      481 tacgaccttc gcattacgaa ttataagaat ccgcttgtaa ttcaaaggat tatcccatca
      541 acactgcgct cacacgtccc accacatcaa aacatgtaaa gccttgcaag ccattgtgag
      601 gccttatgtg tctcagtttt gtctcatcag acatagcaag tatcgatcaa ttgagacttg
      661 gatgatagac ttcatgcctt tcagagctca ttgattaaat aaatgttaaa gttatttgta
      721 aagtacacat ctataggtgt gctgaacaca cttatacact gggtggtttt tggtgtatgt
      781 atctataccg cacataccag tcaggctctg gcaaacttca caggtttcgt agtggctgtg
      841 agctttagct tcttcgcgaa tgcaagattc acattcaaag catcgactac agcgatgcgc
      901 tacatgttat acgtgggatt tatgggaata ttgagtgtga ttgttggttg ggcagctgat
      961 aaatgctcac ttcctccaat agtcactctt atcaccttct ccgccatcag tctggtgtgc
     1021 ggtttcgtct attcaaagtt cattgtcttt agggatgcga aatgaaaata tctcttgtcg
     1081 ttcctgtctt caatgaagaa gaagcgatac ctgttttcta taaaacggta cgtgaattcc
     1141 aagagttgaa gccatatgaa gtagaaattg tattcataaa tgacggaagt aaagatgcca
     1201 cagagtcaat tattaacgcg ctggctgttt cagacccgct agttgttccg ctgtcattta
     1261 cacgcaactt tggtaaagaa ccagccttat ttgcagggtt agaccatgca agcggcgatg
     1321 ctgtaattcc tattgatgtc gacctgcaag acccaattga ggttatccct catcttattg
     1381 aaaagtggca ggcaggtgct gagatggtgc ttgctaaacg ttcagaccgc tcaactgatg
     1441 gacgactgaa acgtaagaca gctgagtggt tctataaatt acacaacaaa ataagcaccc
     1501 caaagatcga ggaaaatgtc ggagattttc gactcatgtc tcgtgaggtt gtggagaaca
     1561 ttaaactgtt gcctgagcgc aatcttttca tgaaaggcat actgagctgg gtgggtggtc
     1621 agacggatgt cgttgaatat gtacgcgcag agcgtgttgc tggcatctca aaatttaatg
     1681 gctggaaatt atggaatctg gcactggaag gtatcacaag tttttcaacc tttcctcttc
     1741 gcgtatggac ttatataggc ttgtttgttg caagcatttc atttttatat ggtgcatgga
     1801 tgattataga cacccttgtc tttggtaacc cagtacgcgg gtatccctcc ctgcttgtat
     1861 caatactttt cttgggtgga gtgcaactga tcgggattgg tgttctcggt gaatatatag
     1921 gtagaattta cttagaaaca aaatcaagac ctaagtatat tttgaagaaa ggtagaaaat
     1981 aaccatgagc atatgtatta aacaatcagc attaaaaata ttattggctt tatcagcact
     2041 cctaataact tggttgacta ccagatactt tccagttgaa ccggatgttg ctaacagccc
     2101 aattgtatgg aggcatattt tagagaatgg tatatcatca atccacgact ggaaaccaac
     2161 cgtggataat tggtatttca ccgtttaccc aattaacttt ttattttata tgctgttagg
     2221 tgatgatggc ttagtagcat taagattatc aactgcagtt ttctctattg ctatagtaat
     2281 agcagcgatg ttaacgctac gtaaggcatt cgggtttacc cctgctttat tttctattat
     2341 acttctatct ctaataccat atttttcata cacttatggt tttgtatcac atcctttttc
     2401 tcataattca acaaacgctt tcggcttttt atgcttgctt atatctgttt tcaacataca
     2461 gtataaaaat atatttatca ctctcctctt aagtttgaca gcattatttt ccagcgtatc
     2521 cgatccgtgg tttacagctg cattttttat tccactatta atatcttact ttttattctc
     2581 tgtatgggat aaaaaattat ttaagcatac tgcattaata ctatttgcat gtttaatctc
     2641 tctttccaat gtattgcaaa atctacttaa catacctccc caccaatttg aaattgtatc
     2701 actcaatgat atgattttaa atgcaaaatg gtgcattctg ttaataggaa aaagtcttaa
     2761 tttattagtt gtcgacaata acgcaacatc ttacgcctca tttgtcattt ggttcattgc
     2821 cattattaca tctgcgtggt ttgttttatc ggacaataaa aaaaacacat accgtattta
     2881 tattgtgtta ttttcattat tatctatcgc aggtattgtc tcatcattca tactgagcta
     2941 taaatcccct gattatatta gcatgcgttt ctttatgaat gttacctgtt tcgcactgat
     3001 attgtgctgt atcggtacat caacaaaagc aaagatatta ttttatctaa tcgcattttt
     3061 attttcgatc agttcaatta aatcttacac aaataatgct tcaccattac acgatcagga
     3121 gaagattgtt aaatcataca ttgatttttt aaaaaaaaat aatctccatt atgggtacgg
     3181 ctctttttgg gatttgtcaa tgactgtaaa ttggttatct ggtggggaca tacagataac
     3241 tcctgttttc tttaatgctg attcggggaa aattaacttt actggcgtga gacaacagac
     3301 actggcttct tggcatagca aggaggcgtt caattctgct cctgagcgac agtttatagc
     3361 agtttccata gctaacgaac ctgaacgatg taaagaaatg acatcctgcc ttgcaggtat
     3421 tcaagaacaa ttaggtaagc ctgacgaagt tttgaacttt gaaggaagag taatattagt
     3481 atttaacaaa aaacttaacc tatagtaaat tgccccctac catataaacc acactaacga
     3541 atgaataacc cgtctttttc gacgggttat atccctatca ggaataaaaa tggagtggtt
     3601 gccgtataca acaaaccctc atttagagct atattctaga gtatatgaga tactataaag
     3661 ttgaaggagc tatcattaga aaaaccatat gctgttataa ttagatgtct cgcttttcat
     3721 ccaataacga attttctctt atagattgct ctctggtttt ttcggccact ctgtattttt
     3781 tatatcaaca cgcatcaata gtgcgcgata ttttttccat gccagtaaca gtttcgtttc
     3841 atcatctgtt gccatatcta aatcaaccgc atcctgaagc ggcgctatta cattcgttgc
     3901 ctctgctatc agtgcagatt tcttttcatt atttattaag gctaattgtt ctggtgttgg
     3961 agaaggacgg tcaacaatga cagggtgacc attctcacca caggtaatca tttttgttac
     4021 actctgcgaa ttaatcagtg ttttccactc tgattccgag atttccactg catcatcagg
     4081 aatattggtg ccgtgaaatt cagtcgaata aaatccgttt gtcgatgcag aataaaaata
     4141 tgacatttcg ttaatatccc attgcaatgt aatacgcatc agcagatgaa ccatatcctg
     4201 aaccgggcga cactgtaata tcaaatccag tgttctggac atttgtcgcc gatacagtcg
     4261 gctgccctga cgcgccagta acgttcaatg taactgtgac agcaaggact cttgagaatg
     4321 tcatcgggaa cctcatcccg tatgttgaag atacattagg ggagccattc aactgaccgc
     4381 actgtaaaat taatccagac ggaaattttt gcgcagcagc cgttgcggta ttacttgacg
     4441 cgaacaggct catatcaggt atctgattcg cccctgtacc tacattcctt ttagctgctt
     4501 ctcccaaacc aaggttttcg agagccgttt tcaccgtgcc atccgatttg atatcgccaa
     4561 acggattctt gcggcttaac agcagcgcgc gaagtgcggt aagcaactga tcgtgccgcc
     4621 ccttctccag gctggcaccg gatgcctcca ccacgctgca aa
//
LOCUS       DAEEUE010000194         4636 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.194, whole genome shotgun sequence.
ACCESSION   DAEEUE010000194 DAEEUE010000000
VERSION     DAEEUE010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.194"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..311
                     /locus_tag="KLK84_004021"
     CDS             <1..311
                     /locus_tag="KLK84_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBE2011139.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA
                     "
     gene            468..1217
                     /locus_tag="KLK84_004022"
     CDS             468..1217
                     /locus_tag="KLK84_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HBE2011140.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1239..1805
                     /gene="yieF"
                     /locus_tag="KLK84_004023"
                     /gene_synonym="chrR"
     CDS             1239..1805
                     /gene="yieF"
                     /locus_tag="KLK84_004023"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HBE2011141.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="KLK84_004024"
     CDS             complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="KLK84_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HBE2011142.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLMSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3361..4017
                     /gene="yieH"
                     /locus_tag="KLK84_004025"
     CDS             3361..4017
                     /gene="yieH"
                     /locus_tag="KLK84_004025"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HBE2011143.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSAIEGAGALLSAITAPMC
                     VVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCI
                     LVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWDITA
                     "
     gene            4084..4557
                     /locus_tag="KLK84_004026"
     CDS             4084..4557
                     /locus_tag="KLK84_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HBE2011144.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1238 a         1162 c         1138 g         1098 t
ORIGIN      
        1 cgctggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactgcc gttggttgct cttcctctcc
      181 cgtttgacga aagccagcaa aaaaagctgg aagttccttt taccctgctg tggcataaac
      241 ggaacagcca taatccgaag atcgtctggt tacgggaaac cattaaaaac ctttacgcgt
      301 cgatggcata accgaatcgt atgaaacgcg acccaatttc acaataaaat gtaaaaaagt
      361 tgtaataagc ttgtctgaat cgaactttta gccgctttag tctgtccatc attccagtaa
      421 atgattactc ttgtattcat aatggaccat taagcatgga gcgaaaaatg gcgactcact
      481 ttgcccgagg gattttaacg gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc
      541 atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg tccagaggtt
      601 tactcgcaga actgatgttc atgctgtatg gcattggcga attgccggaa atcgtcaccc
      661 tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc ttcgttttct atttcctatg
      721 ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga atgtggcctc gatatggaac
      781 tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc cgataaccac accttttcca
      841 gcaatgaatc gctatggatc agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca
      901 ttacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg
      961 atctgcagct gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag
     1021 cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc ggtcacgccc gcgattgaaa
     1081 agctcagtgt ctgggagtta gatggcaaac acagctggaa aagcctgccg gatattcaca
     1141 gccgcgccaa caatcctacc agccggatga tgcgttttgc ccagctctct accgtgaagt
     1201 ctttttcgcc aaattgatag acaaccacag gagtcattat gtctgaaaaa ttgcaggtgg
     1261 ttacgttact ggggagcctg cgcaaaggct catttaatgg catggttgca cgtaccctgc
     1321 cgaaaattgc tccggcgagc atggaagtca atgcgttacc ttccattgcc gacattcccc
     1381 tgtatgacgc tgacgtacag caggaagaag gttttccagc aacggttgaa gctctggcgg
     1441 aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc ggaatataac tactcggtac
     1501 cgggtgggct gaaaaatgcc atcgactggc tttcccgcct gccggatcaa ccgctggccg
     1561 gtaaaccggt gttgattcag accagctcaa tgggcgtgat tggcggcgcg cgctgtcagt
     1621 atcacctgcg ccagattctg gttttcctcg atgcaatggt gatgaacaag ccggaattta
     1681 tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg agaagtgatt gatcagggta
     1741 cgctggacca cctgaccggg caattgaccg catttggtga gtttattcag cgagttaaga
     1801 tctaaataaa aaacccgcca gtaatcatgc atggcgggtt tttaatgcgc tatcgatttt
     1861 aatgagcgtc gataaataca atcttcagga taaacagcag cgcaacgatg attacgcacg
     1921 ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac gcagtaggag ataaagccca
     1981 gcgcaatacc ttcggtaatc gaaaagctga acggcatcat cacggcggta ataaacgccg
     2041 gaacagattc agtaagatcc tgccagttca cgcgagcaag acttgaggtc atcaacacgc
     2101 caacgtagat caacgcgcca gctgcagcgt agcctggcac cattcccgcc agcggcgaca
     2161 gaaagataac cagcaggaac agcagaccaa caaccactgc cgtcagaccg gtacgaccgc
     2221 caaccgatac gccggaagag gactcaatat aagccgtaac ggaagaagta ccgataaacg
     2281 aaccggtcac ggaagagata ctgtcgacat acagcgcctg cttcatgcgc gggaatttcc
     2341 ccttctcatc cgccagacct gctttatcgg tcacgccaat cagcgtaccg gaggagtcaa
     2401 acaagttgac caacatgaaa gagaaaatca cccctgccag cccgaggtta aacgacccgg
     2461 ctaaatctac atgacccaca actgtcatta cgctcggcgg cgcagaaacg atgccattgt
     2521 agtgcacatc acccagcatc cagcccagca gcgtcgtcac cacgatagaa accagcaccg
     2581 ctgcgtgaat gttgcgcgag gccagaatag caatgatgaa aaagccgagg atacccagaa
     2641 gtacgctgtg agaagtcaga ttaccgatgc tcatcagcgt ttccgagtta gcgacaatca
     2701 cacctgcgtt tttcagcccc atcatgccga taaacaggcc gataccgctg gtaataccca
     2761 cacgcagact taccggaatg ttagcaatca tccagtaacg aacgcggaaa atggtcaaaa
     2821 gaagcagtcc gacagcgccc cagaagattg cgcccatacc gatctgccac ggcagcccca
     2881 ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc catcgcagga gccagcgcca
     2941 ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa agcagcaatc agacaggttg
     3001 ttacgaagac ggcactggtg tccatgccag caacgcccag aatttgtggg ttaacaaata
     3061 cgatgtagac catcgtcagg aaggtggtga acccggcaat cacttcagtt cgtgccgtag
     3121 taccatgttc gcgcagttta aaaacacgtt ccagcatcct ctgaccagaa gtctgggtgg
     3181 tatgttgatg actcattatc tatttccgaa caaggaggga aaatccgtcg ctatcgtata
     3241 ccaaaatgcg acaataggcg cgtttgtgag agactttttt attggattta cttatacggc
     3301 aacgattgcg ttgcgcaaat cggctctacg aaaacgttaa actgattaaa aaggaaaggc
     3361 atgtcccgga tagaagcggt atttttcgac tgcgacggta cgctggtcga cagtgaagtc
     3421 atttgctccc gcgcatatgt aacgatgttt caggaatttg gtattacgct cgatcctgaa
     3481 gaggtattca aacgtttcaa aggtgtaaaa ctgtacgaaa ttatcgatat tgtttccctt
     3541 gaacatggtg ttacgttagc gaaaacagaa gctgaacacg tttaccgtgc agaagtcgct
     3601 cggctgttcg attcagccat cgaaggggct ggagcgctcc tgtcagcgat cactgcgcca
     3661 atgtgtgtgg tatctaacgg cccaaataac aaaatgcagc attctatggg caagctgaat
     3721 atgttgcact acttcccgga taaactgttc agcggctacg atattcagcg ctggaagcca
     3781 gacccggcgt taatgttcca tgcggcaaaa gcgatgaatg taaatgtaga aaactgcatt
     3841 ctggttgatg actcagttgc cggtgcgcaa tctggtatcg acgcaggtat ggaagtgttc
     3901 tacttctgcg ccgacccgca caataagccg atcgttcacc cgaaagtcac cacctttacc
     3961 catctttcgc agttaccgga actgtggaaa gcgcgtggtt gggatattac ggcatagttc
     4021 ttcacactcc cttcacttac cccgcttaaa ttggcgctca aaggtaagta aagggagttt
     4081 gatatgtctg tttcacgtcg ggtaatacat cacggacttt attttgcggt tttaggaccg
     4141 ttaattggtg ttctgtttct tgtcctctac atattcttcg caaaagaacc gctggttctt
     4201 ttggtaataa tacaggtact tccattgttt ctcttattgt cgattactac gggagctatt
     4261 cctgcattgt taaccggcgt gatggtcgcc tgtctgccgg aaaagatcgg gtcacagaaa
     4321 aattatcgtt gtctggctgg tggcataggt ggcgtcgtta tcaccgagat ctattgtgca
     4381 gttattgtac atattaaggg catggcttcc tcggagttgt ttgaaaacat tctttctggt
     4441 gacagtctcg ttgtccgcat cattcctgca ttgctggcag gtgtggtgat gagcagaatc
     4501 attacccgtc tgcccggatt ggatatttca tgtcctgaaa cagactcttt aagttaagcg
     4561 ggatacttta tctttgggct actcaaaagc agacaggatg ttactatgac tcaaaatatc
     4621 aggccgttac cccaat
//
LOCUS       DAEEUE010000195         4614 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.195, whole genome shotgun sequence.
ACCESSION   DAEEUE010000195 DAEEUE010000000
VERSION     DAEEUE010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.195"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            225..2648
                     /gene="pgaA"
                     /locus_tag="KLK84_004027"
                     /pseudo
     CDS             225..2648
                     /gene="pgaA"
                     /locus_tag="KLK84_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
     gene            2657..4150
                     /gene="pgaB"
                     /locus_tag="KLK84_004028"
     CDS             2657..4150
                     /gene="pgaB"
                     /locus_tag="KLK84_004028"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HBE2011145.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPVDEQVKFGDEMVD
                     REYFATWQQVREVARSRLVEVASHTWNSHYGIQANSTGSLMPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQKIITVQEKSPQRIMHIDLDYVYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNHLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIR"
     gene            4154..4614
                     /gene="pgaB"
                     /locus_tag="KLK84_004029"
                     /pseudo
     CDS             4154..4614
                     /gene="pgaB"
                     /locus_tag="KLK84_004029"
                     /inference="COORDINATES: protein motif:HMM:NF026233.1"
                     /note="in Yersinia this protein is involved in biofilm
                     formation and hemin adsorption; related protein PgaB in
                     Escherichia coli is an outer membrane N-deacetylase
                     involved in poly-beta-1,6-N-acetyl-D-glucosamine (PGA)
                     export; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase"
BASE COUNT         1319 a         1035 c         1061 g         1199 t
ORIGIN      
        1 atttatgccg tattcctgaa gatcctcatc attggaatgg attttcgggc gagaaaagga
       61 ttttatatgg acactctgct catcatttct tcttctcatc atcaacaatt cacgtctctc
      121 ttccgcgttt aataacggat tatgaggtgc aaaaatatct ttcttttcag ttacctgtaa
      181 ttagatacag agagagattt tggcaataca tggagtaata caggatgtat tcaagtagca
      241 gaaaaaggtg cccgaaaacc aaatgggctt tgaaacttct tactgccgca tttttagcag
      301 cgagtcccgc ggcgaagagt gctgttaata acgcctatga tgcattgatt attgaagctc
      361 gcaagggtaa tactcagcca gctttgtcat ggtttgcact aaaatcagca ctcagcaata
      421 accaaattgc tgactggtta cagattgcct tatgggccgg gcaagataaa caggttatta
      481 ccgtttacaa ccgctaccgt catcagcaat taccagcgcg tggttatgca gctgtcgccg
      541 tcgcttatcg taacctgcaa caatggcaaa actcgcttac actgtggcaa aaggcgctct
      601 ctctggagcc gcaaaataag gattatcaac ggggacaaat tttaaccctg gcagatgcgg
      661 gtcactatga tactgcgctg gttaaactta agcagcttaa ctctggagca ccggacaaag
      721 ccaatttact cacagaagcc tatatctata aactggcggg gcgtcatcag gatgaattac
      781 gggcgatgac agagtcatta cctgaaaatg catctacgca acaatatccc acagaatacg
      841 tgcaggcatt acgtaataat caacttgctg ccgcgattga cgatgccaat ttaacgccag
      901 atattcgcgc tgatattcat gccgaactgg tcagactgtc gtttatgcct acgcgcagtg
      961 aaagtgaacg ttatgccatt gccaatcgcg ccctcgccca atacgctgca ttagaaattc
     1021 tgtggcacga taacccagac cgcactgccc agtaccagcg tattcaggtt gatcatcttg
     1081 gcgcgttatt aactcgcgat cgttataaag acgtcatttc tcactatcag cgattaaaaa
     1141 agacggggca aattattccg ccctaggggc aatattgggt tgcatcggct tatctcaaag
     1201 atcatcagcc gaaaaaagca cagtcaataa tgaccgagct cttttatcac aaggagacca
     1261 ttgccccgga tttatccgat gaagaacttg cggatctctt ttacagccac ctagagagtg
     1321 aaaattatcc gggcgcgcta actgtcaccc aacataccat taatacttcg ccgcctttcc
     1381 ttcggttaat gggcacgcct acgagcatcc cgaatgatac ctagttacag gggcattcgt
     1441 ttctctcaac cgtagcaaaa tatagtaatg atcttcctca ggctgaaatg acagccagag
     1501 agcttgctta taacgcacca ggaaatcagg gactgcgcat tgattacgcg agtgtgttac
     1561 aagcccgcgg ttggcctcgt gcagcagaaa atgaattaaa aaaagcagaa gtgatcgagc
     1621 cacgtaatat taatctggag gttgaacaag cctagacagc attaacgtta caagaatggc
     1681 agcaggcagc tgtcttaacg cacgatgttg tcgaacgtga accgcaagat cccggcgttg
     1741 tacgtttaaa acgtgcggtt gatgtacata atcttgcaga gcttcgtata gctggttcaa
     1801 caggaattga tgccgaaggc ccggatagtg gtaaacatga tgtcgactta accaccatcg
     1861 tttattcacc actgctgaag gataactggc gcggttttgc tggattcggt tatgccgatg
     1921 gacaatttag cgaaggaaaa gggattgttc gcgactggct ggcgggtgtt gagtggcggt
     1981 cacgtaatat ctggctcgag gcggagtacg ctgaacactt tttcaatcat gagcataaac
     2041 ccggcgcgcg cctgtctggc tggtatgatt ttaatgataa ctggcgtatt ggttcgcaac
     2101 tggaacgcct ctctcaccgc gttccattac gggcaatgaa aaatggtgtt acaggcaaca
     2161 gtgctcaggc ttatgttcgc tggtatcaaa atgagcggcg taagtacggt gtctcctggg
     2221 ctttcactga tttttccgac agtaaccagc gtcatgaagt ctcaattgag ggtcaggaac
     2281 gcatctggtc ttcaccatat ttgattgtcg atttcctacc cagtctgtat tacgaacaaa
     2341 atacagaaca cgatacccca tactacaacc ctataaaaac gttcgatatt gttccggcat
     2401 ttgaggcaag tcatttgtta tggcgaagct atgaaaatag ctgggagcaa atcttcagcg
     2461 caggtgttgg tgcctcctgg caaaaacatt atggcacgga tgtcgtcacc caactcggct
     2521 acgggcaacg cattagctgg aatgacgtga ttgatgctgg cgcaacgcta cgctgggaaa
     2581 aacgacctta tgacggtgac agagaacaca acttatacgt tgaattcgat atgacattca
     2641 gattttaagg ataaatatgt tacgtaatgg aaataaatat ctcctgatgc tggtgagtat
     2701 aattatgctc accgcgtgca tcagccagtc aagaacatca tttataccgc cacaggatcg
     2761 cgaatcttta ctcgccgagc aaccgtggcc gcataatggt tttgtggcga tttcatggca
     2821 taacgttgaa gacgaagctg ccgaccagcg ttttatgtca gtgcggacat cagcactgcg
     2881 tgaacaattt gcctggctgc gcgagaacgg ttatcaaccg gtcagtattg ctcaaattcg
     2941 tgaagcacat cgaggaggaa aaccgctacc ggaaaaagca gtagtgctga cttttgatga
     3001 cggctaccag agtttttata cccgcgtctt cccaattctt caggccttcc agtggcctgc
     3061 tgtatgggcc cccgtcggca gttgggtcga tacgccagtg gatgaacaag taaaatttgg
     3121 cgatgagatg gtcgatcgag aatattttgc cacgtggcaa caagtgcgag aagttgcgcg
     3181 ttcccggctc gttgaggtcg cttctcatac atggaattct cactacggta ttcaggctaa
     3241 ttccaccggc agcttaatgc ctgtatatgt aaatcgtgca tattttactg accacgcacg
     3301 gtatgaaacc gcagcagaat accgggaaag aattcgtctg gatgctgtaa aaatgacgga
     3361 atacctgcgt acaaaggctg aggtaaatcc acacgttttt gtttggcctt atggcgaagc
     3421 gaatggcata gcgatagagg aattaaaaaa actcggttat gacatgtttt tcacccttga
     3481 atcaggtttg gcaaatgcgt cacaattgga ttccattccg cgagtattaa tcgccaataa
     3541 tccctcatta aaagagtttg cccagaaaat tattaccgta caggaaaaat caccacaacg
     3601 gataatgcat atcgatcttg attacgttta tgacgaaaac cgccagcaaa tggatcgcaa
     3661 tattgatgtg ctaattcagc gggtgaaaga tatgcaaata tcaaccgtgt atttgcaggc
     3721 atttgctgat cccgatggtg atgggctggt caaagaggtc tggtttccaa atcatttgct
     3781 accaatgaaa gcagatattt ttagtcgggt tgcctggcaa ttacgtaccc gctcaggtgt
     3841 aaacatctat gcgtggatgc cggtattaag ctgggattta gatcccacat taacgcgagt
     3901 aaaatactta ccaacagggg agaaaaaagc acaaattcat cctgaacaat atcaccgtct
     3961 ctctcctttc gatgacagag tcagagcaca agttggcatg ttatatgaag atcttgccgg
     4021 acatgctgct tttgatggca tattgttcca cgatgatgct ttgctttcag attatgaaga
     4081 tgccagtgca ccggctatca cggcttatca gcaagcaggc tttagcggga gtctgagcga
     4141 aattcgataa aacccggagc aatttaaaca gtgggcccgc tttaaaagtc gtgcgttaac
     4201 tgacttcact ttagaactta gtgcgcgcgt aaaagccatt cgcggtccac atattaaaac
     4261 tgcacgaaat atttttgcac ttccggtaat acaacctgaa agtgaagcct ggtttgcaca
     4321 gaattatgct gatttcctaa aaagctatga ctggaccgct attatggcta tgccttatat
     4381 ggaaggtgtc gcagaaaaat cggctgacca atggttaata caattgacca atcaaattaa
     4441 aaacatccct caggctaaag acaaatctat tttagaatta caggcacaaa actggcagaa
     4501 aaatggtcag catcaggcta ttccttcgca acaactcgct cactggatga gcctattaca
     4561 actgaatgga gtgaaaaact atggttatta tcccgacaat tttctgcata acca
//
LOCUS       DAEEUE010000196         4601 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.196, whole genome shotgun sequence.
ACCESSION   DAEEUE010000196 DAEEUE010000000
VERSION     DAEEUE010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.196"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..395
                     /locus_tag="KLK84_004030"
     CDS             <1..395
                     /locus_tag="KLK84_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011146.1"
                     /translation="LEYATTRKKSEVVYSGVSVTIPTAPTNLVSLLKTLTPSSGSLAP
                     FFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQLTFSGSVPDTLVSSRNAATT
                     TDNILLATFFSVDKDGFLATTIKIIAEQ"
     gene            395..850
                     /locus_tag="KLK84_004031"
     CDS             395..850
                     /locus_tag="KLK84_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HBE2011147.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            853..1461
                     /locus_tag="KLK84_004032"
     CDS             853..1461
                     /locus_tag="KLK84_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HBE2011148.1"
                     /translation="MLYAFKLGRKLCGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALASLLGTSTPWGAGIGAGIGLLGSLF"
     gene            1471..2802
                     /locus_tag="KLK84_004033"
     CDS             1471..2802
                     /locus_tag="KLK84_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HBE2011149.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            2803..4533
                     /locus_tag="KLK84_004034"
     CDS             2803..4533
                     /locus_tag="KLK84_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBE2011150.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDALQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNL
                     DPIEKRVEVA"
BASE COUNT         1261 a         1135 c         1204 g         1001 t
ORIGIN      
        1 ctctggagta tgcaaccaca cgcaagaagt cagaggttgt ttactctggt gtatctgtaa
       61 ccatcccgac agcgccgacc aaccttgtta gcctgctgaa aacgctcaca ccgtcatccg
      121 gctcgttggc accattcttc gacaccgtta acaacaagat ggttgtgttc aacgagaaca
      181 aaaccctgct cttcaagttg tcgattgtcg gcacgtggcc cagcggaacc gccaacaggt
      241 caatgcagct aaccttttcc ggctctgttc ctgacacact ggtcagcagt cgtaatgcgg
      301 cgacaacaac cgacaacatc ctgttagcta cgttcttcag cgtggataaa gacggctttc
      361 ttgccacaac catcaagata atcgcggagc agtgatgatt acattcaaac caacgcgaaa
      421 catcgacctg atagaagcag tcggaaatca ccctgacatt atcgccggga gcaacaacgg
      481 tgatggatac gactacaaac ctgaatgccg ttactttgag gtgaacgtgc acggtcagtt
      541 tggcggcatt gtttactatc aggagattca gccgctgaca ttagattgcc acgccatgta
      601 cctgccagag attcgcggct tcagcaagga aattgggctg gcgttctggc gatacattct
      661 gactaacacc accgttcagt gcgtaacatc gttcgctgca cgcaaattcc gacacggtca
      721 gatgtactgc gcaatgattg gccttaagcg tgtaggaacc atcaagaaat acttcaaagg
      781 cgtggatgac gtgacttttt acagcgccac acgcgaagaa ctaatcgact tcctgaatca
      841 cgggagatag ccatgttata tgcatttaag ctgggcagaa aactgtgcgg cgaggaacct
      901 tattgccctg aaaaaggcgg gaaaggtggc agttctgata aaagcgcaaa gtatgcagca
      961 gaagcccaga agtatgctgc agacctgcaa aatcagcagt ggcagacgat catgaaaaac
     1021 cttgctccgt tcacgcctct tgcggagcag tatgttaacc agcttcagaa cctttccagt
     1081 ttagaaggtc aggggcaggc acttaatcag tattacaact ctaagcagta taaagacctt
     1141 gcagggcagg cgcgttacca gagtcttgct gctgcggagg cgacgggtgg acttggttcg
     1201 acagccacaa gcaatcaact ggctacgatc gcgccgacac tcggtcagtc ttggttatca
     1261 aatcagatga gcaattacaa caatctggca aacgttgggc ttggtgcgct gcaaggtcag
     1321 gcaaacgccg ggcagacgta cgccaacaac atgagcagca ttgcacagca aagcgcagct
     1381 cttgccagcc ttttgggaac atcaacgcct tggggcgctg gtattggtgc tggtatcgga
     1441 ttgcttggct cgttgtttta aggggtaatc atggctactt ggcaaggaac aaacggcgga
     1501 ttgttggctg gtatcggcgg cgtcaactca aacgctccga gcgtaaatga catcggcaat
     1561 acgcttcagc ttatcaggca gaacaatgat attgagcgtt caggcgctaa caatgttggg
     1621 ctgactgctt tgcaaggtct ttcaggtatt gcgggtgttt ttcagcagga aaagcaggct
     1681 cagcggcaga aagaatttca gcaggcgtac gctaatgctt atgcgtctgg tgatcgcggt
     1741 gctttgcgtc agttggctac tcaatatcca gaccagattg aatccgttcg taaaggcatg
     1801 ggattcattg atgaagagca gcgtaattct atcggcacct tagcggctgg cgcacgcctt
     1861 gcgtcatcgt ctccagaagc aatgcaatca tggctgcaaa acaacgccgg tgagttagct
     1921 cgtgttggcg ttaatcctca tgacgtcgct cagatgtacc aacagaaccc gcggcagttc
     1981 ggcgaatttg tcgatcacct ggggatgaac agtctcgggc ccgaaaaata ctttgaccta
     2041 caggataaaa tgcagggtcg ccaggttacc atgcgcggtc aggatctgga ttcgcaaacc
     2101 gccgctcgga atcaggcaat cacaatgcgc ggacaagata tccaggcgaa tttaggtcag
     2161 cagcgcatta atctggacgc agaaacaaac cgcattaaca acgaaaataa gcgccttgac
     2221 cggatgctat cagcagaaac taacgacctg aagcgccagg aaatacagag ccgcatagca
     2281 gccaacaacc agcagttgca gcagaagcag caagcgctaa atgatggcta caaagacggc
     2341 atcaacaccc tcacaaccag catgttcact ctgaacgata tcgttagttc tccttcactt
     2401 aagagcatta caggcttacg tggagtaatc cccaacgttc caggctcaca ggctgcagac
     2461 actcaggcac gacttgatac ctttaaatca caagcatacc tgacagcggt tcaggccatg
     2521 cgaggcatgg gcgcactttc tgatgccgag ggcaaaaagc tcgaccaggc tgttggttcg
     2581 ctgcagaact cgcagagcga ggagtccttt cgtcgcaacg ctggcgtcat cctgaacacg
     2641 ctcaaccaga agcgtaatga ggcggttggt aagtacgttc agcaaaacgg tatcaagcga
     2701 gtggaagcgc ctcaggcttc tatagattac ctgaagcagc accccgagct gtcaatcgac
     2761 ttcattaatc gctacggata tcttccatct ttggggcagt aaatggctaa ttaccgtgat
     2821 ttgttagagc aggctggcgc acgttacggt gtgccagaag ggttgatgac tgcactgggt
     2881 gccaaggagt cttcttacaa ccctgccgca gtaagctccg ccggggctgt aggattgact
     2941 caggtcatgc ctgggacatg gcgtgatatg ggttataccg atgagcaaat gcaaaacccc
     3001 gaatatcagg ctgacgctgg cgcgcgctat ctggcaaaga tgtaccagca gtttggtaac
     3061 tggcgtgacg ctcttcaggc ttatcacgac ggtcccggca acgttatgaa ggcaaagcgt
     3121 ggtgaatata cgccaggacc tgaaggccgc ggttacgttg atgatcgctt tgctcaatgg
     3181 gctggtgacc cggtgacaga ctcaacagtc gaacagcgcg ccacctctgc aaaggtacat
     3241 cctcagcaag accctaacaa cccgtttgca caactggaag tacagtcatc cgaacaagta
     3301 tcggcatcag gtgtgcagtc agacccaaat aatccatttg ctcagattga gcagcaggca
     3361 gccagtcagc agccacctca acccgtaagt tctgtcgcac cgaaacctgt tcagcaacaa
     3421 acagttaatc aggccaataa tgcaccaaca cgggaagaac catcattgat gcaacaagct
     3481 ggcgattggc tcactggtgg tcaaagtgca gggcaaattg cagaacaggc tggtcgtggt
     3541 ctggtaaaca taccatttga cgtattgcag ggtggcgcaa gtctgattaa tgcaatcagt
     3601 caggggcttg gggggccaaa agtattggat gatgtttatc gtccagtaga cagaccgaca
     3661 gacccctacg cgcaagctgg agagtcaata ggcggttatc ttgttcctgg agcaggtgta
     3721 gctggaaaca tggtcattgg ttctctcgct gacgcggcga atcaacgggg tgattttgcc
     3781 gaaaatgccg ctattaatgc cggacttagc attgctacgc atggcctgat aaatggcgtt
     3841 acccgtggtg ttcgtggtgc atcaaatata attagtggca ataaaacatc tgcacagaga
     3901 gcgaccactg cgccaacaga aacatcacca ttctccggtg atgccgctgc agcaacaaat
     3961 cctgcggttc atgccgcaga ggcaagagta gcacaaggtg taccaatgac gcctgcgacg
     4021 aagaacccag aggaagtcgt tcgcacagta gcagcacaaa aaaggccaaa tctcgcttca
     4081 tcgcttgatg aactagatat caatcctcag gctgaagttc tggagtctgc tgaaaggctt
     4141 aatgttgatt cattactccc ttcacacttt tccgggaacg agcaatacaa ggcagttgag
     4201 caagcaatca agtcccgtgc gggttctgct ctacaggtgc aggaaaatga agcaatcagg
     4261 cagctagcac agggcgcggg tgagataatt gatcgcgttt ccggtgcaaa agatgctctt
     4321 ggtatgagcg acaagtttat tgatacggtc aatggaagaa tgtctgcgct gatgaaacga
     4381 agcgaccagc tttatcgcaa tgttgaaaag gcgatgcctg caggtgcaaa aattgatgcg
     4441 ccatcaacaa ggtcaatgct caaacaggtg gcagaagatc ttggcgggat gaaaaacctt
     4501 gaccctattg aaaagagagt tgaggtagcc tgagtttaac ggacactcct tcctgaaata
     4561 gaatggcatc agaaggagct aataatgagc agaaaaaccc a
//
LOCUS       DAEEUE010000197         4557 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.197, whole genome shotgun sequence.
ACCESSION   DAEEUE010000197 DAEEUE010000000
VERSION     DAEEUE010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.197"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            291..851
                     /locus_tag="KLK84_004035"
     CDS             291..851
                     /locus_tag="KLK84_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
                     /protein_id="HBE2011151.1"
                     /translation="MTTVQFLYLLIIERDLQPTDWCSFMEKDYLRISSTVLVSLLFGL
                     ALVLVNSWFNQPGVEEVVPRSTYLMVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILS
                     LAFLSCLIYFVKTVIIIQQIIEGRLTSSVVQNDIAIYYLFRQMSLCILIFLALMNKVS
                     ENTKQRNLFSKKMTLCISLFLFLGGR"
     gene            794..1183
                     /locus_tag="KLK84_004036"
                     /pseudo
     CDS             794..1183
                     /locus_tag="KLK84_004036"
                     /inference="COORDINATES: protein motif:HMM:NF028467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
     gene            1163..1720
                     /locus_tag="KLK84_004037"
                     /pseudo
     CDS             1163..1720
                     /locus_tag="KLK84_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420326.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            1818..3427
                     /locus_tag="KLK84_004038"
                     /pseudo
     CDS             1818..3427
                     /locus_tag="KLK84_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(3528..4019)
                     /locus_tag="KLK84_004039"
     CDS             complement(3528..4019)
                     /locus_tag="KLK84_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FidL"
                     /protein_id="HBE2011152.1"
                     /translation="MTQRYFLFSGIILCAFIAAILSHIAFHHSNEPAEQNISCNAHVI
                     NFTDHTKMDQYFSLNMISENNTGNMYLTGAYTVDGKKMGFIRRYVSFTYKKFRDTVYL
                     TTVKIQKIQKDDNISDEILENITSDFFIKADTSINYYVTRQNDYNYIFSTGRTPRFIC
                     ITH"
     gene            complement(4024..4200)
                     /locus_tag="KLK84_004040"
     CDS             complement(4024..4200)
                     /locus_tag="KLK84_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001411776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBE2011153.1"
                     /translation="MLSPIIEKLQKMEVNCKIHPYIYIATNDSMSGFYAFTCSQMLNS
                     ESPNNCNSLYYKAE"
BASE COUNT         1399 a          728 c          901 g         1529 t
ORIGIN      
        1 cccaatgcct ttttcaaaat aaattaggat taaaataatt aaatcagtaa attccgatgc
       61 atgatttcgg atttttcgaa agtcctgatg aaaggctgcg ctacggcact cacggattta
      121 attgttattc ctaatgtatc tatgaattaa tgttttataa ggatttttta taaccattta
      181 taagtatttt caagcctggc ttgttgcaaa caatgtataa agcacttagg caataataat
      241 tacattcagc aactatcatc atcggtattg tttgtgggcg gaatttcata ttgacaacag
      301 tacagttctt atatctatta ataatagaaa gagatctaca acctacagat tggtgtagct
      361 ttatggaaaa agactatttg agaattagta gtactgtatt agtgagctta ttgtttgggc
      421 ttgctttggt tttggtgaat agttggttta atcagccagg cgttgaggaa gttgttccac
      481 gatcaaccta tctgatggta atgattgctt tattttttat cgatactgtt gcatttattt
      541 ttatgcagtt gtatttcatt tatgaccgta ggcaattttc aaactgtata cttagtttgg
      601 cttttctgag ttgtttgatt tactttgtta aaaccgtcat tatcattcag caaattattg
      661 aggggcgttt gacaagcagt gttgtccaaa atgacattgc aatctattat ttgtttcgtc
      721 agatgagttt gtgcatatta atatttctgg cattgatgaa taaagttagt gaaaacacaa
      781 aacagcgcaa tttattttcc aaaaaaatga ctttgtgcat aagtttgttt ttgtttttgg
      841 ggggccgata gttgctcaca tactttctag tcattatgag agctacaatt tacacattgc
      901 cgagttaacc aatgaaaatg gtcaggtggt ttggaaagcc tcatatgtta ccataatgat
      961 tttcatgtgg ttaaccttgc tttcggttaa tctatacttt aatggtttac gctatgatat
     1021 ctggaatgga gtgacggtga ttgctttttg tgccgtactg tacaatatct ctctgttatt
     1081 tatgagcagg tatagcgtgt ccacttggta tattagtcgc actattgaag tagtcagtaa
     1141 gttaaccgtt atggtaatat ttatgtgcca tatattcagt gcgctacgag taacaaagaa
     1201 cattgcacat cgcgatccct taaccaatat atttaacaga aattattttt ttaatgaact
     1261 gacagttcaa tcagcatcag ccaaaaaaac gccttattgt gtcatgatta tggatatcga
     1321 ccacttcaaa gaagtcaacg acacctgagg gcatccggtt ggcgatcagg tgataaaaac
     1381 agtggtgaat atcattggga aaagcatacg accagatgac cttttagcgc gcgtcggcgg
     1441 cgaagagttt ggcgtcttgc tgacggacat cgatactgaa cgtgcgaaag ctttagcgga
     1501 aaggattcgg gaaaatgttg agcgtttaac tggcgataat cctgaatacg ctataccaca
     1561 aaaagtgacg attagtattg gcgctgttgt gactcaggaa aatgcgttaa acccgaatga
     1621 gatctatcga ctggctgata acgcactata cgaggcgaaa gagaccgggc gtaataaggt
     1681 ggttgtgagg gatgtggtga gtttttgtga gtcaccataa agcggcattt tagcgggtga
     1741 ttttggtaag cgctaaatag ctattcgcag acttgcgtaa caattttcag aaatatcata
     1801 gtactatatg atcttaaatg cttatcattg atcaatctgc tatcacccgc ttttactggc
     1861 aattgtacta taatttctta ggttgcatca ccctgatgcg tggggaaaca tgtatggtat
     1921 tcaacaaaaa aatgtttgta ctcattataa taccaggcat tctgggggtg ttactatcgt
     1981 ttgctatgtc aatatttcaa atgaatagag atacgacaat taccgccggt attctgttaa
     2041 aacagcttga taatgttacg caaattgtcc agcatacgac aaagcttacc tctattctgg
     2101 tgatgaagcc ctgcaaagac attcttgaac agttgattgc taacggagca ttgactccct
     2161 atgttcgaac aacgggtctt atagaaaata atttccaaat atgttcttca gttagcggtt
     2221 tcaaaaaaat gaatgttaac gatgtttacg gtacgagctt tcacaataaa aataaagaat
     2281 cacgtatagt atcaattagc ggtacaagtt ttgtgccagg taaaacagca atagtgttcc
     2341 ttatgccaat agggaatgat atgacagctt ttagtattgt agagtcacga tatatctatg
     2401 atctaatgga tgttcttgat gatgagaatg atgattcatt ttctcttcgt tttactgaag
     2461 ggcctgcaat tattagcggt gtaaataata atgacaggtt atatatgtta aaaagagatt
     2521 ttaattctgc gatcagtcag gctcgtctta cagttactac gccgatgata tcgttatatc
     2581 cctatatcat tagcaatgtg ctttatattt tgccgttatc tattattctc tcttttattc
     2641 tctatttctt atggcagcac tggatgtcac ggaaaatgtc gctggcagaa gaaattaaaa
     2701 aaggaatgtc atccggagaa ttttctgtgc attatcaacc agtgtgcgac acgacaacca
     2761 aagcgtgtct gggggttgaa gcgttaatgc gctggcaacg cgaagatggt aaaaatatat
     2821 ctccggtagt ttttatccgc gctgcagaag aggaaaattt aataataccg ttaactaaac
     2881 atctctttga actgattatt caggatgtac aaagctggaa agtgaaaaag ccgtttcacc
     2941 ttgggataaa tattgccgcg gaacatcttg cacatcctga ctttgttggt gatgttttac
     3001 atattaaaaa tgcaatatct gataaattta atattgtcct tgagattacc gaacgcaatc
     3061 tggttgaaga cactgatctt gcattacaga aaataaatga attaaggatc catggctgtg
     3121 aatttgcagt cgatgatttt ggcacgggat attgttccct tggccttctg caaaagttgt
     3181 ctgttgatta tcttaaaata gataaaagct ttatagacac acttactaca gcggaagacg
     3241 aaacgccagt tttagatatt attattaagc tgagtaatcg gctcaatttg ataacgattg
     3301 cagaaggtgt cagtactcct caccagcaga atatcttatt cataataacg tgacattagt
     3361 tcagggatac ttatatgaaa aaccaatgaa agcgacagag ttttatcagt ggtatgtaaa
     3421 cagataaata attaaaaata ttggaatggt tttgtattgt cttaaaatat aaacaaagca
     3481 atcagaatgt tattaactga tgacaaacgc caagatcaac aatgctgtta gtgagtaata
     3541 catatgaatc gtggtgtcct gcctgtagag aatatgtagt tataatcatt ttgacgcgta
     3601 acataataat taattgaagt gtctgctttt atgaagaaat cagaagtgat attttcaagg
     3661 atttcatcgc tgatgttatc atctttttgt attttttgga ttttaactgt ggtgagataa
     3721 acggtatcac ggaatttctt gtatgtaaaa ctcacatatc ggcggatgaa gcccattttt
     3781 tttccatcta ccgtgtatgc gcccgtgaga tacatgtttc ctgtgttatt ttcagaaatc
     3841 atatttaacg aaaaatattg atccattttt gtatgatcgg tgaaattaat aacatgggca
     3901 ttacatgata tgttttgctc tgctggttca ttggaatgat gaaacgcaat atggctcaga
     3961 attgctgcaa taaaagcaca taaaattatt cccgaaaaca gaaaatatct ttgagtcata
     4021 tgattactcc gctttataat agagagagtt gcaattattt ggtgattcag agtttaacat
     4081 ttgactacag gtgaaagcat aaaacccgct catgctatca tttgttgcaa tgtaaatgta
     4141 aggatgtatt ttgcagttga cttccatttt ctgcaatttt tcaataattg gtgatagcat
     4201 gtctacggaa ttaaagtaat tattatcata gaaataactg catccttcct gagtagggtg
     4261 ttgagcataa tttgagaaag catctatttt ttttatagtg tgagatttgc tttgaaaagc
     4321 catatcgaaa aaataagaag acatataaac tatcccccat gccgcaaaaa aaaactataa
     4381 gtaatgtatt ctttgtggta aatatacctt ttgcaacgta atttttattt atattttcag
     4441 tgtgggtgcc attctcttca ctctcaacac tttcattctc attgtgatta ttgtcgagaa
     4501 cagttatgtt gatttcttca ttgattttga agcctttccg tggcaccgtt ctgataa
//
LOCUS       DAEEUE010000198         4551 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.198, whole genome shotgun sequence.
ACCESSION   DAEEUE010000198 DAEEUE010000000
VERSION     DAEEUE010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.198"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..1225
                     /locus_tag="KLK84_004041"
     CDS             <1..1225
                     /locus_tag="KLK84_004041"
                     /inference="COORDINATES: protein motif:HMM:NF027197.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBE2011154.1"
                     /translation="FPYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLY
                     PLSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGIS
                     NYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGV
                     TNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEK
                     WFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILP
                     VIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHL
                     LIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRA
                     ARVFVNDERFTRGGN"
     gene            1225..1899
                     /locus_tag="KLK84_004042"
     CDS             1225..1899
                     /locus_tag="KLK84_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE2011155.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPA"
     gene            2427..3188
                     /locus_tag="KLK84_004043"
     CDS             2427..3188
                     /locus_tag="KLK84_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBE2011156.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3232..4311
                     /locus_tag="KLK84_004044"
     CDS             3232..4311
                     /locus_tag="KLK84_004044"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBE2011157.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            4308..>4551
                     /locus_tag="KLK84_004045"
     CDS             4308..>4551
                     /locus_tag="KLK84_004045"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBE2011158.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQNATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSISDFGTQ"
BASE COUNT         1249 a          865 c         1119 g         1318 t
ORIGIN      
        1 ctttccgtat ggcattttcc agcagcgaaa tgttcttgcg agctggctgg caacaggttg
       61 cggcgtagcg ttgtatctgg ctttaacggc gcgtactcgt tctctgacgt tgatatgttt
      121 gttgtcgtta taccctctgt ctacagcttt agtccttaca caaagccgtg tcggccttct
      181 tggtttattt tgcgtcatcg tgctggccgc tctcgcggac agaccccgct ggcgcggacg
      241 tccccttagc ggtctgttgc gcttgatctt cgtcgcctcc ttgctgtgct ggtgtgtcgg
      301 tatctcaaac tatgccatgc caggggagac aacggctgac tttacgcatt catcatcgaa
      361 tgaccagcgt atacgtattc tgaaaggtgc cataagtctt attcaacagc gccccttaac
      421 cggttggggg ttaggcagct ttgaagcgac cttcccgcaa gccttagagg atgtgggcgt
      481 gacaaacata gagagtgata cggctacgca tccccataac gaactgctgt tcgtaacggc
      541 tgaaggtggc atcgtcgcct tgtttggttt tttgctgcta tcaggcgttt ggctgtggcc
      601 gtgtatatgc cgcctgaaaa tgacaaacaa taacgcgcac catcaatgtg aaaagtggtt
      661 tattgcctta atggggttgc ctttagtcat tcatatgatg accgaatatc ctctctatac
      721 gtctgttccc catttgatgc tgttgctgct ccttttccgc atcggaatgc cggaaaacgt
      781 gatgaatcag gtacgtatac ctaaaacagt ccggttgggt attctgcctg tcattgtgct
      841 cattatgacc gccgcgctgg tcatcctggt agcgggattt gatgcacagc gggaactgac
      901 cagagctgaa gctgatatga acgaaggatt tatccctcgc cttccggaac atagctggaa
      961 atacctgacg caggcagaac gtcttgagcg cgatcagcat ctgcttattg cgaataaacc
     1021 ggggttcgtt cgcgatacgg aaagcatgtc tcttttcact gtgtggggaa ataaatggtt
     1081 ggcaatccac aatgatgcag acgtaagcgc tgcaatgata ttcattgcgc acaggcgcgg
     1141 tgattatctg acagctgaaa aaataagaaa acgcgcagct cgggtatttg taaatgacga
     1201 acgttttact cgcgggggaa actaatggtt aacgatctga cgtataagaa attagaaagc
     1261 acaaacatca actgtaatat tctctcgcca gaaaatacca catggacaaa tgtcacggtg
     1321 attggcctgg atctttttgc catttgcggc gtgtgcagtt tgctcaagcg tgttggggga
     1381 gtgaaacttc aggttttcac ttccttaccc actgatatga atgttctgac tgacagtagc
     1441 atcgtgatat gggtaaaaat gcgtcacgat ggtttgcctg aattagccgg acatattgtg
     1501 aaggtttgtc gtcgataccg tctgatgcgg caactcgtta ttagcgatgc tattcctgaa
     1561 ggaatgacga atgatatcgg gcctctttcc cgagtatggc ttactcatgg gagtgcaaaa
     1621 gtggatattt tattatcttc attacggaag gtgttaaatg ctcattgttt gcctggtcct
     1681 ttgtttataa aacgcctggg aagagtgcaa tggagggttc ttttactccg cgcgaagggg
     1741 attagcacac gtaaaattgc agaaatctgt ggtattggcc tcaagaccgt tagcgcccat
     1801 gagtccgcaa ttcgagaacg attaggcacc aggaataaaa gtgagtacgt ctggttagta
     1861 cgtaatgctc aacttataca acaggcgata cccgcgtagg gaagagatgc gtcaaggtat
     1921 gaaagatgta acaatatgcg aacaaagcaa aaagtataat tgatgcgtca tagataactc
     1981 tctttttcga tgacgaaatc gctgagcatg cgtggtgagg ttagtaatag atgggtgcta
     2041 tgttttgcca cgggtttgta gctgtgaaac ctggaaaaaa ataagaatta aaaatattta
     2101 aagagagtta ataaggttct ggttgtaatt gtcttggtca aaaaaaagat attgtcaatg
     2161 ttgtttttta aattttcagg ttttggttaa atatttgctt acaaatatag gtgatttttt
     2221 tattgtaggc ttttgaaggc tattataact gtgcgctaaa ttaatgcgtt gaataaaaga
     2281 aataacaaaa ttaagaaaag ccatatttac gatatgggca acataaatat aatctaaaaa
     2341 agagtaaggc gttgtaggtt ggttccgcgt tcattacctt gttagtgaac gcggatttat
     2401 cagttgtcga aatggttttt ataataatga aaaattttaa tgtactaacc aaagtcgtat
     2461 tttgcttgtc gtttttatca agcgcatttc ccacctatgc tgcggatgat gtcaaagtga
     2521 ctcaaaacac ttcagttttt aaactgaagc ttagtgcaac gcgattaatc tatgaacaag
     2581 ggtcggatgg cgcagcgata actgtgacaa atccgcagga ttggccgatg cttgtgcaga
     2641 gtaaagtctt tgaagaagat aaagtaactg cagcgccctt tattgtcact ccgccactgt
     2701 tcaggcttaa tccaggccag caaaataaaa tgtgggttgt gcagactgcg ccagctttac
     2761 aagacaggga aaaattacac tggctatgcg ccaccggcat tacgcctaaa aagagtgatg
     2821 tttgggccga gaaagataaa gaaaaaattg ctgaaaatgt gaacctgaac atccaggtta
     2881 acgtacatac ctgcataaaa cttattacca ggccatctgg tattgaaggc aagatggtta
     2941 atgcgagtga gaagttatcg tggaaaaagc agggtgataa aattgtcgcg gtcaatcagt
     3001 cgccgttcta catcaacctt tcttctttac aggttggcgg tagcaagatt gagaaactag
     3061 attatattgc gccgttttcg caacatgagt ttgttatgcc aaaaactagc ggccaggata
     3121 ttaaatggtc tgtgattact gatgaaggtg gtgagagtcg tttgtttata tctcatttgc
     3181 aatcataatg tattacattt ttattgaata tttttatgcc atgcatattc catgttttat
     3241 cgactgtcat taattttgat aatggcctta cttgcgggtc agttaagtgc ccaggagtgg
     3301 tcatttgatt cttcacagtt ggaaggaaac gttagtgcgg acactgtggc aatgtttaat
     3361 cagggagagc aactccccgg aaattatcgt gtggaaattt atcttaacgg tgaaaaagtt
     3421 gatgttgggg agtttccctt tcatcgtccg gaatcccctg aagaaaaaga gcttgttcca
     3481 tgtttaaccg ttgatgacct tatccactat ggcattaaaa ttgataaaag cagcagtgat
     3541 actgacaata aaaagaatca gtgttttaaa tggaactcta ttgaaggttt gaaagtaaat
     3601 tatgattttg atagccagcg cgtacagata accgttccac aactgtatct gcaagataaa
     3661 aaaagtagtt tagcaccagt gtcgttatgg aatgagggtg tagctgcatt ccgcatggtg
     3721 tatcagacaa atattgatat ttccaaacaa aatgataatc aatctaccac gcgtaattct
     3781 cgttatggca ggttcactcc agggtttaat cttggtgcct ggaggtttcg tagttcagtg
     3841 acatggagca aggaacttgg ccagtcagaa agatggcaaa ggggttacat gtggtttgag
     3901 cgaggcataa atgcgattaa aagccgatta acgcttggag aatcttatac gtcgtctgag
     3961 gtgtttgaca gcataccttt tcgcggcggg atgttagcga ccgacgatgc gatgactccg
     4021 ccggaggaca gctactacac acctgtggtg catggaatag cacaaagtga ggcgcaggtt
     4081 attattaaac aaaatgggca aattattttt acccgttctg tgccgccagg gccttttgcg
     4141 ctggataatt taccaacgct ggccgttggc ggggagcttg atgttacagt gagagagagt
     4201 aatggcgagg agcagtattt ttccgtgcca tttcaaacgc cggccatcgc tctgcatgaa
     4261 ggatatttca aatatagtgt gatggggggg aatataaaga agaaggtata aaaagctccc
     4321 ctgtctccgt gggcgaaatc acggcgatgt acgggcttcc ttatggcgtc acggtgtatg
     4381 gagggattca aaacgccact cattttaatg caatatctac gggtattggc atttctctgg
     4441 ggctgttagg gagtttatcg acagatatta cgcgttcgat tgcgaattta tattatggga
     4501 ataaatatcg tattcgttat agtaaatcca ttagtgattt tggtactcaa t
//
LOCUS       DAEEUE010000199         4535 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.199, whole genome shotgun sequence.
ACCESSION   DAEEUE010000199 DAEEUE010000000
VERSION     DAEEUE010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.199"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..299)
                     /locus_tag="KLK84_004046"
     CDS             complement(<1..299)
                     /locus_tag="KLK84_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBE2011159.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQSEYDSQWATICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTTAERQRLKELERENRELRRSNDILRQASAYFAKA"
     gene            353..794
                     /gene="tnpA"
                     /locus_tag="KLK84_004047"
                     /pseudo
     CDS             353..794
                     /gene="tnpA"
                     /locus_tag="KLK84_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317271.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(912..1856)
                     /gene="hypT"
                     /locus_tag="KLK84_004048"
                     /gene_synonym="qseD"
     CDS             complement(912..1856)
                     /gene="hypT"
                     /locus_tag="KLK84_004048"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HBE2011160.1"
                     /translation="MVMKNIETKWLYDFLTLEACRHFSQAAKERNLSQPAFSRRIKAL
                     EAAIGVVLFDRTTTPLQLTEEGKLFHSQTRSLLQQLECNLGELNGQSLLGVPNIKIAA
                     AHSLSLSVLPKLVHSLTAYGGEFVYHVEAIDVVQAVNTLREGKSDFIISFRDEDLMQS
                     PFCCLKLFESELYPVCAADAQGHPVFDITQPQVPLLNYTATSYMGRLVNRHLAEVGGI
                     TGRTIFISSMSELLKNMALNGYGIAWLPIWSIVDELQTKRLICLDAAKLTVPIQAYIY
                     RMNTRLNRTAENLWRILQEHMPDDLIQQISMEEPARRN"
     gene            2201..2920
                     /locus_tag="KLK84_004049"
     CDS             2201..2920
                     /locus_tag="KLK84_004049"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBE2011161.1"
                     /translation="MKGLIGVLGGMGPAATVDLFNKFVTFTAAQCDQEHIPLIISSIP
                     DIPDRTDALMHHGRSPLPVMRDYMHKLEDAGAECIVIPCNTAHYWFKELKDACHIDIL
                     SIVETTINEVRACGKTRIGLLATNATLYMGLYQKGIESLGFTCVSPDADGQKKVMESI
                     YSLKAGNIAHAQKLMNEQAEILFSRGAEILVLGCTEIPVILAQAVKEQPSRYIDSTAS
                     LVRAGIKWYENRVGKHYLLTQ"
     gene            2986..4284
                     /locus_tag="KLK84_004050"
     CDS             2986..4284
                     /locus_tag="KLK84_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HBE2011162.1"
                     /translation="MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMG
                     SPPVDVILIIMTVVVAASALQAAGGMDYLVRVAGNFMRRNPKYINIIAPIITWSMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
                     NGVSFIQVMSVCLPTSFAAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNSSI
                     KPGATLSVGLFLLATVAIVILAAFPQLRPGFDIGKPMETRDIIIICMLSAACLMVVLC
                     KTATDAIILTSTFRAGMSSLAVILGIVTLGTTFIDAHLTEIKDIAGDILQAYPMLLAL
                     VLFGTCALLYSQGATTPLIIPLAVALDVPTWAILASYVAVTGVFVLPTYPTSLAAMEF
                     DTTGTTRVGKYVLNHPFMLPGLGGIIAGVALGFVIAPMII"
BASE COUNT         1131 a         1007 c         1118 g         1279 t
ORIGIN      
        1 gccttcgcaa aataagcgga agcctggcga aggatatcgt tactgcggcg cagttcacga
       61 ttttcacgtt ccagctcttt cagacgctga cgttcagcgg tggtgagccc tccatcaccg
      121 cccccggtat cccgctcatg ctggcgaacc cagacacgca gagtctccgg cgtacagcca
      181 atctttggag caatggaaca aattgtcgcc cattgtgagt catattcgct ctgactttcc
      241 agaaccatac ggactgcccg ttgacggact tcgggggaaa aacgagtatt tttagtcatc
      301 ctgtttacct ctttctcagg aagtttagtc tccaggattc ccggggcggt tcatagcgca
      361 cacccgatgg aactgtaaat atcacatagt tttttgcgcc aaaataccga agacaggtgt
      421 tctacagaga gaagcgtaga gcaacaggca gtattttgag aaagctgtgt gagtggaaaa
      481 gcgtacggat tctggaagct gaatgctgtg cagatcatat ccatatgctt gtggagatcc
      541 cgcccaaaat gagcgtatcc ggctttatgg gatatctgaa agggaaaagc agtctgatgc
      601 tttacgagca gtttggtgat ttgaaattca aatacaggaa cagggagttc tggtgcaggg
      661 gggactacgt cgatacagtg ggtaagaaca cggcgaagat acaggattac ataaagcacc
      721 agcttgaaga ggataaaatg ggagagcagt tatcgattcc ctgtccgggc agcccgttta
      781 cggaccgtaa gtaacgaagt tggatgcaaa tgtcagatcg tgtgcgcctg ttagggcgcg
      841 gctggtaaga gagccttata ggcgcatttg aaaaacctcc ggctatgccg gaggatattt
      901 attgcacgtc gtcaattgcg gcgtgctggc tcttccattg atatctgctg gattaaatcg
      961 tctggcatat gttcctgtaa aatacgccag aggttctcgg cggttctatt cagtcgggtg
     1021 ttcattcggt aaatataagc ctgaatgggg actgtcaatt tagcagcatc aaggcatatc
     1081 agacgtttgg tctgtagttc atcgacgatc gaccagatcg gtaaccaggc aatgccgtat
     1141 ccgttgagcg ccatattctt taataactcg ctcatcgaag agataaatat cgtgcgcccg
     1201 gtaatacctc caacctctgc cagatgacga ttgaccaacc tgcccatata agatgtggcg
     1261 gtatagttga gcaaaggaac ctggggctga gtgatatcga atacagggtg cccctgtgcg
     1321 tcggcggcac ataccgggta caactcagat tcaaacagtt tcaggcagca aaaaggcgat
     1381 tgcataagat cttcatcgcg aaacgaaata ataaagtcac tcttgccttc gcgtaacgta
     1441 tttacggcct ggacaacatc aatggcttca acatgataga caaattctcc gccataggcg
     1501 gtaagtgaat gcaccagttt gggtaatacg ctcagggata gtgagtgcgc ggctgcaatt
     1561 ttaatgttgg gaacgccgag taaactctgg ccgtttaact cccccaaatt gcattcaagc
     1621 tgctgtaata agctgcgagt ctgggaatga aagagcttgc cctcttcggt taattgtaaa
     1681 ggggtggtcg tgcgatcgaa taaaacaacg ccgatcgctg cctccagcgc cttaattcgt
     1741 cggctgaatg cgggttgcga aagattacgc tcttttgccg cctgagaaaa atggcgacag
     1801 gcttccagtg tcaggaaatc gtataaccat tttgtctcga tattcttcat taccatggca
     1861 cctgtatttt caatccaaag acataataaa acatcccggc aataaaaata atgttctcaa
     1921 ttagcacaac ctggccttta tcattcgttt tgtgcataac cccttcattt ccggtattga
     1981 cgttgatatc acatttcgcc aaaaaaacga aatttttccg tcagataacc agaatacatc
     2041 atcactatgg tgaataagcg gaatgttcca tagctgcatc atgcgtgtct tgcatcgcct
     2101 tgtacaacct tgcatttctc atcgcccatt tcgactgtta aaaaagagag cagcagttat
     2161 ttaccgcgta ttgtcattaa cgttttgaga ggcaggtatt gtgaaaggtt taattggtgt
     2221 actcggtgga atggggccgg cagctaccgt cgacctgttt aataaatttg taacttttac
     2281 cgctgcacaa tgtgatcagg aacatattcc tttaattatt tcttcgatcc cggatattcc
     2341 tgaccgtacc gatgcattaa tgcatcatgg gcgttcacca ttacccgtta tgcgtgatta
     2401 tatgcataag ctggaagatg ccggggcaga atgtattgtt attccctgta ataccgcgca
     2461 ttactggttc aaagaattaa aagatgcttg tcatattgat atattaagta tcgtagaaac
     2521 tacgatcaat gaagttcgcg cctgtggtaa aacccgaatt ggcttgttag ccaccaatgc
     2581 cacgctatat atgggtttat accaaaaagg aattgagtcg ctgggattta cctgtgtcag
     2641 ccccgatgcc gacggtcaga aaaaagtgat ggaaagtatt tattccttga aagctggcaa
     2701 tatagcgcac gcacaaaagt tgatgaatga gcaggctgaa atattatttt cccgcggtgc
     2761 agaaatcctt gttcttggtt gtacagaaat tccggttatt ctggcgcaag ccgttaaaga
     2821 gcagccttcc cgctatattg actcaacggc gtcactcgtt cgtgccggaa taaaatggta
     2881 cgaaaatcgt gtcggtaaac attatctttt aacccaataa tcatttaaat cgcagccaga
     2941 aagttattcg cttttaactg aattatattt ataacggaga acattatggt ttggctggaa
     3001 attatcgtag tacttggtgc aatatttttt ggtattcgcc aggggggaat cggtattggt
     3061 ttatgtggcg ggcttgggct tgccattctg actctgggat ttggtctgcc tatggggtcg
     3121 ccgccagtcg acgtgatcct gattattatg accgttgttg tggcggcctc agccttacag
     3181 gccgcaggcg gaatggatta tctggtacgc gtcgctggta attttatgcg gcgtaacccg
     3241 aaatatatca acataatcgc gccgattatc acctggtcta tgaccattat ggccggtact
     3301 gggtttattg ttttttccac gctgccggtg attgctgaag tggcgaaaga gtccggtatc
     3361 cgtccttccc gcacgcttgc cggttcggtt gtcgcttcgc aggttgcgat ttccggttcg
     3421 ccgatcagcg ccgcaatggc ggctatgctc actattatgg aggtaaacgg tgtcagtttt
     3481 attcaagtga tgtcggtatg tcttcccacc tcttttgctg ccgcgatggt cgccgctttt
     3541 attgcttccc gacagggatg tgagttgcag gatgacgagg tctaccttga gcgcctgcaa
     3601 aaaggactgg tgcaaaaata cgagaataat agcagcatca agccaggtgc gacgctctcc
     3661 gtcggattgt tcctgctggc gacggtcgct attgttattc ttgctgcctt cccccaactg
     3721 cgtccgggtt ttgatatcgg taaaccgatg gaaacacgcg atatcattat tatctgtatg
     3781 ctctctgccg cctgcctgat ggtggtgctg tgtaagacgg cgaccgatgc cattattctc
     3841 acctccacat tccgtgcggg gatgagttcg ctggcggtaa ttctcggcat tgtaacctta
     3901 ggcaccactt ttattgatgc gcatttaact gagattaaag atattgctgg tgatattttg
     3961 caggcgtatc cgatgttgtt ggcgctggtt ctttttggca cttgcgcgct gctctactcg
     4021 caaggtgcga ccacgccgtt aattatcccg cttgccgtcg cgcttgacgt gcccacctgg
     4081 gcaattctgg cgtcctatgt ggccgtcacc ggagtatttg tactgccaac gtatccaaca
     4141 tcactggctg ccatggaatt tgatactacc ggcacaactc gtgtaggcaa atatgtgctg
     4201 aatcatccat ttatgctgcc tgggttgggg ggaattattg ctggagtggc attaggcttt
     4261 gtgattgcgc caatgattat ttagcactat caaacatcag tatcgatggt ttatacacaa
     4321 taaagacaga tatggcggaa tatagtctgt tgtaagcgcc aacaaagaat aggggaattc
     4381 cgccatgtct gaacatcttc acaatatggt attaagtgtt gtcgtttaat gatttatttc
     4441 ttggttatgt tgtgtattag ctgcatctat cgctttttga tacctgaggt gtactggcaa
     4501 tagcggacac taccatttgt tcttttttta agcag
//
LOCUS       DAEEUE010000200         4514 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.200, whole genome shotgun sequence.
ACCESSION   DAEEUE010000200 DAEEUE010000000
VERSION     DAEEUE010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.200"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..151)
                     /locus_tag="KLK84_004051"
     CDS             complement(<1..151)
                     /locus_tag="KLK84_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBE2011163.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWATICSIAPKIGCTP
                     ETLRVW"
     gene            complement(203..541)
                     /locus_tag="KLK84_004052"
     CDS             complement(203..541)
                     /locus_tag="KLK84_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HBE2011164.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGG"
     gene            complement(541..2618)
                     /gene="flhA"
                     /locus_tag="KLK84_004053"
                     /pseudo
     CDS             complement(541..2618)
                     /gene="flhA"
                     /locus_tag="KLK84_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2611..3758)
                     /gene="flhB"
                     /locus_tag="KLK84_004054"
                     /pseudo
     CDS             complement(2611..3758)
                     /gene="flhB"
                     /locus_tag="KLK84_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
     gene            3994..4377
                     /locus_tag="KLK84_004055"
                     /pseudo
     CDS             3994..4377
                     /locus_tag="KLK84_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311981.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
     gene            complement(4387..>4514)
                     /locus_tag="KLK84_004056"
     CDS             complement(4387..>4514)
                     /locus_tag="KLK84_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBE2011165.1"
                     /translation="ITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNSVASFC"
BASE COUNT         1118 a         1294 c         1104 g          998 t
ORIGIN      
        1 cccagacacg cagagtctcc ggcgtacagc caatctttgg agcaatggaa caaattgtcg
       61 cccattgtga gtcatattcg ccctgacttt ccagaaccat acggactgcc cgttgacgga
      121 cttcggggga aaaacgagta tttttagtca tcctgtttac ctctttctca ggaagtttag
      181 tctccaggat tcccggggcg gttcacccac cgcctggcac ttcccagata aatcgcaacg
      241 gttctgctgc cgctataccg gaaaaggcca cggtggtacc gctctgcccc tctaattcga
      301 cacaacgaga ttgcgaacac aagcgaaccc gcagtcctgc aggtgtcggg ccgataagct
      361 gataacgcca cgccaccagc gtcatcaatc ctgaagcagg ttgtcgcgca gaaagaggtg
      421 cagacgacat cgactcaccg cgatgattta gcgtaattcc gacactactt gcctgccaca
      481 tcccctcccc ggcggcttgc accagcagcg gaaacaataa tatcgctaat aaggttctca
      541 tcatttaccg ccaattgtcg ccgtcatgcg gatatgtcgg ttatcagaca gttccagatt
      601 cgacaggacc actaactgcg gcaagctgcg gcgcaggaag cgagacaata atggtcgcag
      661 cgcgtggttc accaacaata ctggcggcgc acccagcatc tcctgacggg atagagcttc
      721 ctgagtttgc gccagtaaac gatccgccag ccctggctcc agtcctcccc cgccctgcag
      781 cgcctgtagt aacaaacgtt ccagcggtgt atcgaggcca ataacatgga cttcatcttt
      841 gccaggaaac cactgctggg taatcgcccg tcccaacgcc acgcgcacga cggcggttaa
      901 ttcatgtgga tcgctttgga tgggcgcatg ttccgccagc gtttcgagaa tggtgcgcat
      961 atcgcgaatc ggtacttttt catcgaggag attttgcagc actttatgca gtgtggtgag
     1021 cgtgacgacg ccaggaacga gatcttccgt cagctttggc atctcctggg cgacgcgatc
     1081 caacagctgt tgcgcctcct gacgaccaaa cagctctgcg gcatgctggc taatgaggtg
     1141 gttaagatgc gttgctacca ccgtgctggc ctcaaccact gtgtacccct gaatctgcgc
     1201 ctgttctttt agcgcacttt caatccagat agcattcagg ccaaatgccg gatcgacggt
     1261 cgcctcacca ggtaacgtcc cggcagcggt tccagggtta atcgccagcc agcgccccgg
     1321 ataagcatca ccactgccaa tctccacgcc tttcatcaaa atgcgatagc gggcaggttg
     1381 cagatccata ttgtcgcgaa tgtgcaccac tggcggcaga aatcccatct cctgggcaaa
     1441 tttcttgcgg atactgcgta tacggcccaa caactcacca tcctgctgga aatcgaccat
     1501 cgggatcagt cgataaccca cttccattcc cagagaatct tccagttgta catcgttcca
     1561 cgtcgcttcg acaacggtat tattctctgc catttttacc ggtttgggtt cggcaggcgc
     1621 tttttgttcg cgtccgcgta tccaccaggc cagccccagc aatccggcag tgaacagcaa
     1681 aaataccagg ttcggcattc caggcaccag gccgagtaaa ccgagcacgg cggcgcttaa
     1741 caacataacg cttgggttac tgaaaagctg attcaccatc tgctcgccaa catcctgatc
     1801 ggtgctgaca cgcgtaacga tgccccccgc ggcggtagaa atcaccagcg ccggaatttg
     1861 tgccaccaga ccgtcgccaa tggtcaatag cgtataactt tccgccgcgt gtcccatgct
     1921 catgccagtt gcagcacgcc gaccagcaac ccgccgacaa cgttaatgac catgatgagg
     1981 atcccggcaa tggcatcgcc gcgaacaaac ttacttgccc cgtccattga gccgtaaaaa
     2041 tcggcttcct gagtcacttc ggagcggcgt tttttcgcct catcttcacc aatcaatcca
     2101 gcgttaaggt cggcgtcaat cgccatctgc ttacccggca taccatcgag aacaaagcgc
     2161 gcacccactt ctgctatacg cccggcacct ttggtaatga ccataaagtt gatgatcacg
     2221 agaatgacaa acaccacgat accgatagcg aaattgccac caacgaggaa gtgaccgaac
     2281 gcttcgacca ccttcccagc cgccgccgcg ccggtatgcc cttccatcaa aatgatacgg
     2341 gttgaagcca cgttaagtgc cagacgcaac aacgtagtaa acaacagaat ggtcggaaac
     2401 gcagcaaact caagcgtgcg ctgggtaaac atcgccacca gcaacaccat gatcgacaag
     2461 gcaatattga aggtaaacaa caggtcgagt atgaatgcgg gcagtggcag caccatcatc
     2521 gacaagatca acaggatcaa aatcggtccg gcaaggatct gccattatgt cgatttcagg
     2581 tttgcgggca ggcgcagcat cgcggccaga ttactcatgg gtcggtttct cgttaataaa
     2641 atccagggct gccggcaccg gaagatgagt aggtgtacag ggcgctgtcc accagccaga
     2701 cgccagcgtt tcagttgcca gacccaggcc agcacttccg ccaccgcggc gtacagttga
     2761 cccgggattt gttgaccaat ctccgcatgt cgatacagcg ctcgcgccag cggcggcgct
     2821 tcaagcgtcg ggacgttatt ttcagcgcca atttcacgaa tgcgcagcgc gaccagccct
     2881 gcacctttag cgaccacttt cggtgcgctc attttgtttt cgtcatactg caacgctacc
     2941 gaatagtggg tcgggttatt gacaatgaca tccgctttcg gcacatcggc catcatccga
     3001 cgccgtgcgg cagctcgctg catctgacag atccgtcctt taacatgggg gtcgccttcg
     3061 ccttgtttga actcatcacg aatatcctgc cgcgacattc gcagcttttt caggtggctg
     3121 aagatttgga aaaagacgtc aaatcccacc attggaatga caccaagcac caccagcagt
     3181 gcgcatagcc ctaccaaatc catcgcatta cccatggcgg taatcggtga ctcggccatt
     3241 aagcgcatca tctgcggcca gtgatgccag agaaaaaacc ccgtcacgct gccaaccagg
     3301 atggttttca aaattgcctt aagcaactcc gcgctagtct gtgccgagaa tatccgttta
     3361 atgcccggta gcgggttgag tttggaaaac ttcggctgca aggatttgcc gctaaatacc
     3421 agccccccca gcataaccgg agaaatgagc gccaccagca ccacgccgct aatcagcggt
     3481 agcagcgcca gcatggcttc tctgatcagc agaataattt gcccgaggat cagattcggg
     3541 tctttgataa tactgtgatc aaaatgcagc ccagcggaga gcatgcccga caatcgacgg
     3601 gccagcgaca caccgccaaa ccagataaca ctaacgccca ccagcaaaat cagcagtgag
     3661 gtcagttcac gggaacgcgg gatttgcccc tcttcccgcg ctttttctag tcggtgaggt
     3721 gtgggggctt ctgttttgtc gtcgctctcg tcagacacgt cgccaatcct ggaaagagta
     3781 ttaagcgtaa atgatgccag agcgcaaagc gttcaatggt ttaagtaagg ggcaaaacag
     3841 gcgggattta gggcgtttat gcaaaaatag gttcagttgc agaacgtttt gcgaaccatt
     3901 ttccagttct gatatttagc catttagcct atagaatcgc actccgcctc tctgttagtt
     3961 tgctaataat taaattatta caaaggtgat caaatggaat ggcaacaatt gcttaactgc
     4021 aaccgtcgga aagataatct aaaaaaaacc aacatagcat ttttacatcc ccttgctagc
     4081 ggaagacaag agatcgagcg ggattacgac cgattacttt ttgctgcacc aactcgtcgg
     4141 cttgcggata aaacacaagt tttcccgtta gatcaaaatg acagcatacg gacgaggctg
     4201 actcattctc acgaagtagc aaactttgct cgcgggatag gaatgaggct cgctttcgag
     4261 atgaaggagc acatctttgg cgaaataccc acggggattg tcgttgaacg cgacgttcct
     4321 gctctgttag cagcgatcgg actggcacac gatctcggta atccaccatt tgggcatctg
     4381 aagtggtcaa caaaaactgg ccaccgagtt agagtttttc cagtatcgat tttccgattc
     4441 gtttgggggt aacccaccgt tatattcgtg cggtcttagt gcactgtaat atccaacgat
     4501 atagtccgtt atgg
//
LOCUS       DAEEUE010000201         4464 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.201, whole genome shotgun sequence.
ACCESSION   DAEEUE010000201 DAEEUE010000000
VERSION     DAEEUE010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.201"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(139..1041)
                     /locus_tag="KLK84_004057"
                     /pseudo
     CDS             complement(139..1041)
                     /locus_tag="KLK84_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            1101..1379
                     /locus_tag="KLK84_004058"
     CDS             1101..1379
                     /locus_tag="KLK84_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011166.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1380..1907)
                     /gene="gadE"
                     /locus_tag="KLK84_004059"
     CDS             complement(1380..1907)
                     /gene="gadE"
                     /locus_tag="KLK84_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HBE2011167.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2706..3278)
                     /gene="hdeD"
                     /locus_tag="KLK84_004060"
     CDS             complement(2706..3278)
                     /gene="hdeD"
                     /locus_tag="KLK84_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HBE2011168.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3533..3865
                     /gene="hdeA"
                     /locus_tag="KLK84_004061"
     CDS             3533..3865
                     /gene="hdeA"
                     /locus_tag="KLK84_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HBE2011169.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3981..4307
                     /gene="hdeB"
                     /locus_tag="KLK84_004062"
     CDS             3981..4307
                     /gene="hdeB"
                     /locus_tag="KLK84_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HBE2011170.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4371..>4464
                     /locus_tag="KLK84_004063"
     CDS             4371..>4464
                     /locus_tag="KLK84_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="inner membrane protein involved in cell
                     density-dependent acid resistance; part of the acid
                     fitness island (AFI) of E. coli; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBE2011171.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAG"
BASE COUNT         1312 a          954 c          943 g         1255 t
ORIGIN      
        1 tttatcgata tcgaagcatt gagcattttt aactcgtttt atcttgaatg caatcggcgc
       61 ataacttatt gaatcccatc aaatcccgtc tttttttacc cattcccggt tatcttgaat
      121 aaattaccta gtcataaata cattctggcg gctaccttca tccactaatg ccgtgacata
      181 catgccaggc agcaagtcac catttgggtt ggggaaaatc gcccgtaacg tcacggagcc
      241 cgtggtttca tcaaccgtcg ggtcggagaa tttcagcgtg ccggtctggc tgtaacgttt
      301 accattttcc agattgagct gtactggcgt actgccctga acctgtttga tttgcccact
      361 ggcgacctct tctttcatgc gtaagaaatc ttgcaccgac tgcgtgagat cgacataaat
      421 cgggtccaga cgttgtacgg taaccagcga atctgcctga ttagcggtaa cgagtgcgcc
      481 gacggtcacc gacgatttcc cgctgacgcc cgtaatcggc gaggtgacat tcgcgtattg
      541 cagattgatc gtcgcctgtt caacagccgc tttggcgacg gtgacattgg cttctgcttc
      601 attcagctgg gtgcgcgcgg tgtcgtaatc ctgacggcta acgtagttgg tcttcagcaa
      661 cgatgcctgg cggttaaagg tgatgcgggc attgctggcg gtagagagcg ctttcgccag
      721 cgagcctttg gcggagttta gctcggcctg taaaggtgca ggatcaatct gatacagcga
      781 atcgccctgg ttcactttat cgccttcgat aaagttgcgt ttaatgataa taccgcccac
      841 ctggggacgt atctcggcaa cttcataagg aacggttcta ccgggcaatt cgctcaacac
      901 attgaccgaa ccaggggaga gtgtgacgac accgacctca ggcgtcatgg cggcggcgtt
      961 ttccgccgat ttgtcatcgc aggcggtgag catcgcgccg cagaataaca acggtattaa
     1021 cagctttctt cttctgttca ttttagtccc tgaaaattct tgagaaataa cggttcttta
     1081 aattgcgcgc gaggagagat atgagggatt tacagaccag cggcatagtg gggttgtcag
     1141 ccagtaaggt gggatacagg cacagtgatc gacatggtga ggtcaacgac aaaaaaaatg
     1201 ttgccgttct gccaacagtt cctgccagca ttcgggcaac gaagggttct actggtggat
     1261 acacatacca ggggaatcaa gtcgttttag cctctccttt agtaatcacc gggggtaatg
     1321 aaatcagcat ctgcatccct cgtcatgcca gccatcaatt tcagttgctt atgtcctgac
     1381 taaaaataag atgtgatacc cagggtgacg atgtcgctca tacgtcttag tttcagcttg
     1441 tacataatgt tgaatttctg aatctttaat gtttgttgcg aaatattgag cgtcgacgtg
     1501 atattgcttt catttttatt tttcagaatc agttgcataa ttttgaactg attatgtgaa
     1561 aaacataatt gtgaattctt atggggcaag tgtttaccat aagtaatctc aagaagattt
     1621 ttgatacttt ctttgcggct aacaaaataa ataggcgctt tagcttttag tttactgatg
     1681 tgataaggtg ccaaaacaat aattctctcg gcatctaatt tctccagaaa tttaatcgct
     1741 tcttcatcaa ggatatgatt gtgataggta tcaatgataa ccttgaaggg tttattgcct
     1801 acttttttga tctctgacag ggaattgatt tttatcattt cgtgattatc tttcaactgc
     1861 caaaagccct gtaaaagaaa agaatctttc gtcatgagaa aaatcataac ttgctcctta
     1921 gccgttatcg tttcgaacat gtcatccttt tcattattta catccttgtc cgaatcgttg
     1981 ttcaatatag taaacgccta taagaacttt gcccttcgtg aactaatacg tctattaaca
     2041 ctaatcaata cttcagattt atccatcacg cactccatat attgatccta agtaagattc
     2101 ctggttgtta tcagcttgta aatagtaaac aaaaagttaa cgtttgcgga ttactgccca
     2161 agaataagtt atcaataacc agtttgtgat ctctgaagaa tattactaaa gttaaaatgt
     2221 aatccgattt aaatatcgag tctccttgtt tcgacttaag ctggcaattg gattgccagc
     2281 tttcttttaa cgcattgaaa ttacctgagt aaataacgca ttcatgtctt caggttttta
     2341 tatagcgtct ggagacacgg atatttatgc aatgtaatca acgccagttg caaaaaaatg
     2401 aacccggatt acttatttaa ccgcccatag catcaacgct tacggaactc tttgttcact
     2461 ttcccacagc acttttaaaa cattaaacct gactacggaa aatatcagcc atgataatgt
     2521 ttgaaatggc taatttgcca tggagtgaaa aaaattagat gaaattcagt aggttgaaat
     2581 aatcactagc aggtaattat ttcaatgata gtgcgcaatt gatctacaac actgcgtagc
     2641 ggagagagta ttaatcggat catagtcaca tcaagtgact atgatccggg tgacaaccgg
     2701 ggtaattatt gctgcttaac gaacaaactg gcgaagctga acaggctggc ggcgctaaat
     2761 atcagttcaa ttcccaccag tgtggaaacc agcgttacag acaccatcgg cgttgcacca
     2821 aggaatatcc aggcaatgac gatatccagc acaccaataa cgagctgtag ccagctgcct
     2881 ttcattgaac gctgacgata ccaactcatc aggcgaataa cccctgcaac acagaacaaa
     2941 ccggcaataa atgccgcaat ggcaaaaatg cccagctccg gtgcacggat gaagaaatag
     3001 ccgatcaata aataggcgac tgcgacgagg aaaccggata ataccggcca gaaattatga
     3061 ctgcggttgc tgaataaccc gacaataagc gcaatacccg agcagattaa taatgcaccc
     3121 actactgtgc ttaaaatatc gccagagacg aacgggaaac tgatacacag caacccgacg
     3181 ataaacagca gcacggcaat aaactggatt gctctgcgat gctttttcag catctccaga
     3241 tcaaacttca aaattgttgc cttatctata tataacatag aaccacccta taaaattaag
     3301 aagaaaatcc cctgctatca atctatgcca aaaacgcgtc taagaatgca gtcgatttaa
     3361 taaaaatttc ctaattgcag tatctgatgc atctgtaact cattgtattg aaataaaaat
     3421 atctgatttt gatattttcc atcaacatga catatacaga aaaccaggtt ataacctcag
     3481 tgtcgaaatt gattcgtgac ggctctttca ctttatagtt gaggatatta cgatgaaaaa
     3541 agtattaggc gttattcttg gtggtctgct tcttctgcca gttgtgagca atgcagcgga
     3601 tgcgcaaaaa gcagctgata acaaaaaacc ggtcaactca tggacctgtg aagatttcct
     3661 ggctgtggac gaatccttcc agccaactgc agttggtttt gctgaagcgc tgaacaacaa
     3721 agataaacca gaagatgcgg ttttagatgt tcagggtatt gcaaccgtaa ccccagctat
     3781 cgttcaggct tgtactcagg ataaacaagc caactttaaa gataaagtta aaggcgaatg
     3841 ggacaaaatt aagaaagata tgtaattccg ggaatgcgtt acatcgtact tccttgcata
     3901 ttgaacaggc cggaatatct tctttaaaag cagctattcc tcctgttcat atataatctc
     3961 tatattgaat gggttacaaa atgaatattt catctctccg taaagcgttt atttttatgg
     4021 gcgctgtagc ggctttgtca ctggtgaacg cacaatctgc gctggcagcc aatgaatctg
     4081 ctaaagatat gacctgccag gaatttattg atctgaatcc aaaagcaatg accccggttg
     4141 catggtggat gctgcatgaa gaaacagtat ataaaggtgg cgataccgtt actttaaatg
     4201 aaaccgatct cactcaaatt cctaaagtga tcgaatactg taagaaaaac ccgcagaaaa
     4261 atttgtatac cttcaaaaat caagcatcta atgacttgcc gaattaatga ggtacaagta
     4321 aaaaggagta gcaagttgag ccatcttgct gctccttttt gcatttttat atgacagcag
     4381 aatttattat acgtcttata ctcgcggcaa ttgcctgtgg cgctattggc atggaaaggc
     4441 aaatgcgcgg caaaggagca gggt
//
LOCUS       DAEEUE010000202         4323 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.202, whole genome shotgun sequence.
ACCESSION   DAEEUE010000202 DAEEUE010000000
VERSION     DAEEUE010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.202"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..157)
                     /locus_tag="KLK84_004064"
     CDS             complement(<1..157)
                     /locus_tag="KLK84_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBE2011172.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTY"
     gene            complement(307..468)
                     /locus_tag="KLK84_004065"
     CDS             complement(307..468)
                     /locus_tag="KLK84_004065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011173.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(465..653)
                     /locus_tag="KLK84_004066"
     CDS             complement(465..653)
                     /locus_tag="KLK84_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HBE2011174.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            913..1248
                     /gene="iraM"
                     /locus_tag="KLK84_004067"
     CDS             913..1248
                     /gene="iraM"
                     /locus_tag="KLK84_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HBE2011175.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1529..1637)
                     /locus_tag="KLK84_004068"
                     /pseudo
     CDS             complement(1529..1637)
                     /locus_tag="KLK84_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(2160..2236)
                     /locus_tag="KLK84_004069"
     tRNA            complement(2160..2236)
                     /locus_tag="KLK84_004069"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2200..2202),aa:Arg,seq:tct)
     gene            complement(2338..2413)
                     /locus_tag="KLK84_004070"
     tRNA            complement(2338..2413)
                     /locus_tag="KLK84_004070"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2378..2380),aa:Ile,seq:cat)
     gene            complement(2558..3379)
                     /locus_tag="KLK84_004071"
     CDS             complement(2558..3379)
                     /locus_tag="KLK84_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBE2011176.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3394..3750)
                     /locus_tag="KLK84_004072"
     CDS             complement(3394..3750)
                     /locus_tag="KLK84_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBE2011177.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3763..>4323)
                     /locus_tag="KLK84_004073"
     CDS             complement(3763..>4323)
                     /locus_tag="KLK84_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBE2011178.1"
                     /translation="AELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPG
                     EQTATSILKQRTKNLAPLPHAHQQQNPQQEKTVVSIAVDPESPESFMKRPKRRRWVNE
                     KYKRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLA
                     FEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT         1187 a         1178 c          856 g         1102 t
ORIGIN      
        1 gatatgtcag cagcccaaac agcagactcc ccagcacgcc tattgccgcc cactgagacg
       61 gggaaaccct gtccagcaac tgcaggaacc agtagcccgt tcccaccgct gacgtggtgt
      121 atgacacacc tgttgtgatt ttttccatct ggtccatacc ccgtctcccg ttatccggaa
      181 gctgacaaca ataaaaaagc caccagttaa ctactgatgg ctctgataac tcatgcaagc
      241 gtctcagacg acccactgac actaccggtg agtttaacga taccttccat ttgactggct
      301 cactttttat gatgatgccg gtgcatttat ctccagcacc agactttcta tctcaacgcc
      361 atacgttgca tttttggtaa tatccgtcag cgtcagtgca tttagtccca ctgccagact
      421 gtcttttatg gcctggaatg ccgggccagt acgatgacgt agtatcactc cggctcagtt
      481 gcaccactga ccaccacatc accttctgct gcaatcgcct gcatcagggt ataaggggtt
      541 atggccaccg gactaccaaa cggctgccag ccctctttca gtttatgtgt cagcttttcc
      601 gcaagatctg acggcggcgc cgccctgaca acatcatagt gtttaaatgc catggttctt
      661 tccaacatct gaaaaataat tctttaaaat acctgacatg taatacagaa aaaacacaaa
      721 accatacctt aaataaaaac ctgattatca agcagatatg catggataaa ctacaagacg
      781 agatataaac caccctgcat ttaaataaac aataaacaac atcagaaaaa taattctgct
      841 ctatggttta attcaaaaat atcatttata cttttcagaa catcaccagc aaggcataaa
      901 caaggaaact aaatgaagtg gattgtgatt gatacagtta tccagccatc atgcggaata
      961 tctttttcag tcatatggag taaaataaaa ttaataatct ggtatcaatc ggatgctttc
     1021 ttacctcctg aaagtatatt tacactgact cacacaggca tcatgctcaa taacaaagtg
     1081 ctacctgtaa ccatttacaa cgtagtacca ttcaataaaa cattctggaa tttaatcaaa
     1141 aacagccagg aatgccctac aaatacagat aacgtattga atgaatgctt taataaccgt
     1201 tgcactctgc aaatatgtcc ttatggacta aaacaacaaa gtccataagg agtttactca
     1261 catctgacaa aatcaatata aacagcccct ccggagaggg gctggagagt ggcgctatgt
     1321 gccattccat ggtgccgggt gcctcccggt gaattcagta ccagcacctg aatccgcgat
     1381 tatcccatat acctactcgc tgattgcccc tccgcacagg gggattcacc atgccagttt
     1441 cttttaacaa actccccgca aaccagacaa cagtcaaccg cctgaattgt gaagtattta
     1501 aaaatttctc ccgctaactg atacccggct aacagtctgg cgttttcttt ttcagcaacg
     1561 ggaaagcaac aaccaccaca cccgccacca gcacaccgtc agccggcact gacattatcc
     1621 ggctgctgca atgccattaa caaaaacagt aagcaatcac tttttaccgt aacaggtgat
     1681 aatccagata tgtatctacc ccagatgagt aatccgaagt tcatccatac cacaggtcct
     1741 ggctattctg ttgtactcct gaacaagagc aaataattct gaattagcaa ccatgaactc
     1801 atcgcaaacc ctctgtatag catcactatt cagaataata acgtctcttc ccgaaagacg
     1861 atcaggagta cagaacaaaa ctgtcaaacg gctgaaggcc tttgctcgtg ctgcattgac
     1921 tatatcaata cgctgcctaa ggatgaaaca ccccgacgcc tcatcaatat tcactctacc
     1981 cacaccatat gaatgataaa tacttaatgc tgaaaaaacc attagaccgt ataacaaata
     2041 ctcaatcaat acttaacaga acttttattt ttgacaaata tataatattt tcaacaatat
     2101 cctgagccag gtatatttca gtataaggct ctgccggaag gaatctggaa gaatgaatat
     2161 ggcgcgctgt actggattcg aaccagtgac cgattgctta gaaggcaatt gctctgtccg
     2221 gctgagctaa caacgctgaa taccgataat ggaccgccat cggggacccg ccccccgcac
     2281 caacaaccct gttatcgtgt cgtctgctct tcctgataag ctaatggcgg tttgtgatgg
     2341 tggcccttgc tggatttgaa ccagcgacct ggcgattatg agtcgctcgc tctcaccact
     2401 gagctaaagg gccgggagca gaataataac ggtccgtaat taatttcgca ataaaaaaac
     2461 ccgctcagcg gcgggttgta gaaactcttc taacgccagg cataaaacgc ccatcattat
     2521 gacgaattta ccacagattc cggaaaaatc aaccttgtta cctagttacc ttttttaact
     2581 gccgctcagc ccatgcttct tcaatatcaa accgggtcac cagcgcatca taaaatttct
     2641 taactgtttt ttcccatgac gcgcgtgtta tctggtttgt cacctcgcat atagcattaa
     2701 atgcctccgt tgatggtagt ctttcatagc cacgaccacc acaacgctgg cagtctctga
     2761 taacaggcat accacgtttt accgactctt cacgatgaat ggcgacacca cgcccacggc
     2821 aatccttaca ggcggtggaa acctcaccct ttccgccaca ctccggacag gcaactttta
     2881 ccacctccct gacttttttc cattcttccc agtaagacgg atacacacct ttcgtacact
     2941 ttgcccatac cggcggctta ccatccggat actggatctt gtttgtaaaa actatgcttt
     3001 caataaattt ttccccatag caacaagggc actgcttttt actcgctgcg ctgcgggcat
     3061 aatcctcaaa agcgtacgaa gccataatgc gcatcactac cggttttatt tctgccggaa
     3121 gttttctcaa cgccgccaca cgatcgcacc gactgagtgc ataatctgcc agtaattctg
     3181 ttgcccgcgc cctgtcattc atactgatgc ccattttccc caggaacgca gaaaaaccca
     3241 tctcagcccg attctgtgtc atgccctgcg cggccatcac atcagtgata ctcagcgcat
     3301 cttttgacgt tgaggccgat gcatcggtca ggccagggga ttttggggag tagtatttcg
     3361 gtaaatcttc cagtttcatt ttttgacctg ctcttaatgc attatggggt aaatcttcac
     3421 ccccagacgt ccaccagata ctggctgacc acgaacgata ttgatttcat caaactgctc
     3481 atcgtccatt aacactcccg catgcgtcag cgcatccagc ggtgctttca ggatattgtc
     3541 caggtcgcga cgacgcttat ccggtggctc tgcaatcacc tttatcgcca gccttccgga
     3601 caggcttaat ttcagccgct gctggcgaac aataagcgcc acagcccggc gataacgctt
     3661 tccctcctcc gagataaaat atgtgctgcc acggcgtcgc cagtaagtgt tcaccgtcgg
     3721 cgggtaaggt aaaaccaaat ctatgagcat cagtcacctc ttttacccaa gcacgccagt
     3781 tgcaaaggcg tgatcaagaa aacgaaaaat taaatcaacc tgagaaccat gcttttcttc
     3841 gaacgccaga ggatccgcat gaagctcgtt gtgatgctcc cgacacagcg gtagcgtgaa
     3901 aatatcgtga gatttggtcc ccattccgcc ctgaccatga ccaatcaggt gatggggatc
     3961 gtcggctggc ttaccacaac acgcacacgg ctgtgtcttc acccagcgtt tgtatttctc
     4021 gttaacccag cggcgacgtt taggtcgttt catgaaagat tccggagact caggatcaac
     4081 ggcaatgctg accaccgtct tttcctgttg tgggttctgt tgctggtggg cgtgaggcag
     4141 cggcgcaaga ttttttgtgc gctgtttcag tatgctggtg gcggtctgct ctcccggtac
     4201 gatgtcgctt tcacggtaca ttgagcggat tttttccgca cgcaacccca gcgaacgacg
     4261 taataccgct tccggtagcg cgtccgccac ctgattgcgg accgcccacc aggataattc
     4321 agc
//
LOCUS       DAEEUE010000203         4248 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.203, whole genome shotgun sequence.
ACCESSION   DAEEUE010000203 DAEEUE010000000
VERSION     DAEEUE010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.203"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            35..916
                     /gene="yddG"
                     /locus_tag="KLK84_004074"
     CDS             35..916
                     /gene="yddG"
                     /locus_tag="KLK84_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HBE2011179.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            1171..2271
                     /gene="ompC"
                     /locus_tag="KLK84_004075"
     CDS             1171..2271
                     /gene="ompC"
                     /locus_tag="KLK84_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HBE2011180.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            2435..2920
                     /locus_tag="KLK84_004076"
     CDS             2435..2920
                     /locus_tag="KLK84_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HBE2011181.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYIDHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            3030..4019
                     /locus_tag="KLK84_004077"
     CDS             3030..4019
                     /locus_tag="KLK84_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HBE2011182.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNN"
BASE COUNT         1319 a          875 c          804 g         1250 t
ORIGIN      
        1 aatttttaac ggcaagagag acaaaacagc gagcatgaca cgacaaaaag caacgctcat
       61 agggctaata gcgatcgtcc tgtggagcac gatggtagga ttgattcgcg gtgtcagcga
      121 ggggctcggc ccggtcggcg gcgcagctgc tatctattca ttaagcgggc tgctgttaat
      181 cttcacggtt ggatttccgc gtattcggca gattccgaaa ggctatttac tcgccgggag
      241 tctgttattc gtcagctatg aaatctgtct ggcgctttcc ttagggtatg cggcgacccg
      301 tcatcaggcg attgaagtgg gtatggtgaa ctatctgtgg cccagcctga caattctctt
      361 tgccattctg tttaatggtc agaaaaccaa ctggctgatt gtaccaggat tattattagc
      421 cctggtcggc gtctgctggg tgttaggcgg tgagaatggg ttacattatg atgaaatcat
      481 caataatatc accaccagcc cattgagtta tttcctggcg tttattggtg cgtttatctg
      541 ggcagcctat tgcacagtaa cgaataaata cgcacgcgga tttaacggaa ttaccgtttt
      601 tgtcctgcta acgggagcat gtctgtggat ttactatttt ctcacgccac aaccagaaat
      661 ggtatttagc acgcccgtca tgattaaact catctctgcg gcatttacct taggatttgc
      721 ttatactgca tggaatgtcg gtatattgca tggcaatgtc accataatgg cggtaggctc
      781 gtattttacg cctgtacttt cctcagcgct tgcagccgtg ctgctcagcg ccccgctgtc
      841 gttctcgttc tggcaaggcg cactaatggt ctgcggcggt tccctgctct gctggctggc
      901 gacacgtcgt ggttaaaata attgccgggt ctacggcccg gcagttaatc agataaattc
      961 cgacaatatt aaaaaataaa tttcaaatac cagtaattca ccttatatct catttcgact
     1021 tcgatcacat atcgataaat aatattattc tatttttctc attgttttta atcaccattc
     1081 tattttaaaa acagtatgtc atttataaat gacgccgttt aaaattcgtt tagaaaatac
     1141 gtttaacgat aattaaaagg attctgttaa atgaaattaa aaatagttgc ggtggttgta
     1201 actggtttgt tagctgcgaa cgtagcacac gctgccgaag tctataacaa ggatggtaat
     1261 aaactcgacc tttatggcaa ggttaccgct ctacgttatt ttactgatga taagcgtgac
     1321 gatggtgata aaacttatgc ccgtctcggc tttaaaggag aaacgcaaat caatgatcaa
     1381 atgattggtt ttggtcactg ggaatatgat tttaaaggct ataacgatga agccaacggc
     1441 tcgcgcggca acaagacccg tctggcctat gctggtttaa aaattagtga atttggctct
     1501 ctggactatg gtcgtaacta cggtgtcggc tacgacattg gttcatggac cgatatgttg
     1561 ccagaatttg gtggcgatac ctggagtcag aaagatgtct tcatgacata ccgtaccacc
     1621 ggtctagcaa cctatcgcaa ctacgatttc tttggcttaa ttgaagggct gaactttgcc
     1681 gcgcaatatc aaggcaaaaa tgaacgtact gacaacagtc atctttatgg tgctgactac
     1741 acgcgtgcca acggtgacgg tttcggtatc tcctcaactt atgtttatga tggctttggt
     1801 atcggtgcgg tatataccaa atccgatcgg acaaatgcgc aggaaagagc cgcagctaat
     1861 cctctcaatg cctccggtaa gaatgcagaa ctatgggcca caggtataaa gtatgatgcc
     1921 aacaacatct actttgcagc taattacgat gaaacattaa acatgaccac ctatggggat
     1981 ggttatatct ctaacaaagc acaaagtttt gaagtggtgg cgcaatatca attcgacttc
     2041 ggcttgcgcc catcactcgc ttacctgaaa tcgaaaggca gagatctggg ccgctacgcc
     2101 gaccaggaca tgattgagta tatcgacgtt ggtgcgacgt atttcttcaa caaaaatatg
     2161 tcgacctatg ttgattataa aatcaacctg attgatgaaa gcgactttac ccgtgccgta
     2221 gatattcgca ccgataacat cgtcgctacg ggcattacct atcagttcta acgtcctctc
     2281 ctgaccagcc cttctctggg ctggtttttg atttaagtgc tgctgtatga ctccttaagt
     2341 aaaaatgagc aattcttgat atttctggtc attcacctca attaatatta ttccatatcc
     2401 tccggggaca tcttactata atatttattg atgagtgatt atgaccaata ttaatacagc
     2461 ttgtgtaaaa aataatgcca gttatcaact taataacgca ttacctaaca aagaaaccat
     2521 ctccagcaat ttttgtgaac gactagaaca atggggtaat aaatcggtta ataatggtga
     2581 agaaagagca attgccgtgg agcgaattaa agaagcttac aattcgaaca tggcatctct
     2641 tgatttgtca tatcttgatt tgagtgaatt accgcctatc ccctcgacag ttaatacgtt
     2701 aaatctggaa aacaattgtc taacttgtct tgacttagct gacaatgcca gtctcgttaa
     2761 tatcaacctc agcgttaata aaattaacac gataaccttt ccaaaagaac caaaactgga
     2821 aaacatttat attgatcata acaatctgga aagtttagat ttaaaaaatc agcattcatt
     2881 ggttaatctg gaagcgcaaa ataatagcct aaaaaaatga tatttctgat agttataaac
     2941 tgaaatttct taatcttgat tataacaagc tagcatcgct ggatgtctcc cggcaagaat
     3001 ctctgattga gttaagtgcc catcacaata tgatcactga ccttatatta cacaatcatc
     3061 ccaggatgaa aacaatcact ttaaacgaca accatattgc acatttaaac gccaaaaaca
     3121 ctacaaaact ggaatattta aacttaagca ataacaattt actgccaacc aatgacattg
     3181 atcaactaat atcatcaaaa catctttggc atgtattagt taacggcatc aacaatgatc
     3241 cacttgcaca aatgcagtac tggactgcag taagaaatat aattgatgac actaatgaag
     3301 tgaccattga tttatcagga cttaatttaa ccactcaacc accagggctg caaaacttca
     3361 cctctatcaa tcttgataat aaccaactca cacattttga tgcaaccaac tacgatagac
     3421 tcgtaaagct aagtctgaat agtaatgctc ttgagtcaat aaattttcct caaggcagaa
     3481 atgtaagtat tacacatata tctatgaata ataatgctct cagaaatatt gatatagata
     3541 ggctttcatc agttacttat tttagtgcgg cacataatca actagagttt gtgcaattag
     3601 aatcttgcga atggctgcaa tacctgaatc tcagccataa tcaattaact gatattgttg
     3661 caggtaataa aaatgaactc ttactgctag atctatccca taataaacta acaagtttac
     3721 acaatgtctt atttcccaac ttgaatacgt tacttattaa caacaatttg ctttctgaaa
     3781 ttaaaatatt ctatagcaac ttccgcaatg ttcagacatt aaacgctgct aacaaccagt
     3841 tgaaatatat aaatcttgat ttcctgactt atcttccatc tatcaaaagt ttaagactgg
     3901 acaataataa aataacccac attgatacta ataatacatc cgatattgga actttattcc
     3961 ccataataaa acagagcaaa aacttaaatt ttttaaatgt ttctgggaag aacaattgac
     4021 ctactatgca gctcatgtta tttaatttgt tttccccagc acttaagctt aatactggcc
     4081 tggcaattct ttcgcctggt gcatttgaag ttcactctga cggaatagat gcggataacg
     4141 aattgtttca ctatactatt aaaaaagcat ataccccata taatatacac acttacaaga
     4201 cagaggaagt tgtaaaccag aggaatataa aagttaaaaa catgacct
//
LOCUS       DAEEUE010000204         4099 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.204, whole genome shotgun sequence.
ACCESSION   DAEEUE010000204 DAEEUE010000000
VERSION     DAEEUE010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.204"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(954..1145)
                     /locus_tag="KLK84_004078"
     CDS             complement(954..1145)
                     /locus_tag="KLK84_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HBE2011183.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESFHDH
                     ADELYRSCLARFGDDGENL"
     gene            1209..1532
                     /locus_tag="KLK84_004079"
     CDS             1209..1532
                     /locus_tag="KLK84_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBE2011184.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLCQLAAIGNNLNQTARKVNSGQWSSGNRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1522..1833
                     /locus_tag="KLK84_004080"
     CDS             1522..1833
                     /locus_tag="KLK84_004080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011185.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELI
                     DATPFAKKYTSGVLSFAEKELPPGGREKVMASFERVLMPGLEKNQYSILWVDAPGQGT
                     A"
     gene            1808..2119
                     /locus_tag="KLK84_004081"
     CDS             1808..2119
                     /locus_tag="KLK84_004081"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBE2011186.1"
                     /translation="MHQDKGRLELNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLV
                     NHYYGLHDPNAPENRRTLTLPDNLPETKQAIAEGVTRGLPCRRDKRPSGCDSGSHRGR
                     D"
     gene            2200..2730
                     /locus_tag="KLK84_004082"
                     /pseudo
     CDS             2200..2730
                     /locus_tag="KLK84_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
     gene            2737..2970
                     /locus_tag="KLK84_004083"
     CDS             2737..2970
                     /locus_tag="KLK84_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HBE2011187.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(3516..3983)
                     /locus_tag="KLK84_004084"
     CDS             complement(3516..3983)
                     /locus_tag="KLK84_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011188.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRVLQKLQIQEVCNNTLWETAKQQLLQKSDGRYFAINSKHL
                     SFPGPGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          995 a          935 c         1190 g          979 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgtccctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggtatt tcatacccgg tatggcgcac ttttcataca
      781 atctgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaaaac ctgctgatgc gccctgacgg gcttgtctgc
      901 tcccggcatc cgctcacagc ttgtctgtga gcggatgccg ggagcagcat atgtcagagg
      961 ttttcaccgt catccccgaa acgtgcgagg cagctgcggt aaagctcatc ggcgtggtcg
     1021 tgaaacgatt cacagatatc agcctgttca tccgcgtcca gttcgttgag cttctccagc
     1081 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgttta
     1141 gtcactgatg cctccgtgta agggggattt ctgttcagga ggtaatgata ccgatgaaac
     1201 gcgagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgct
     1261 gtgagggtaa acagctggcg gtatggatgc ggcgggtctg cctgggggag ccggttgccc
     1321 gttccgggaa actgccgaca ctggcaccgc cgttactgtg tcagctggct gccatcggga
     1381 ataacctgaa tcagacagcg aggaaggtga acagcgggca gtggtcttcc ggtaaccggg
     1441 ttcaggtggt ggccgcactg atggccatcg gggacgagct gcgccggctg cgtctggccg
     1501 tcagggaaca ggggacgcgg gatgatagtt aagtttcatg cccggggaaa aggtggtggc
     1561 agtggtccgg ttgattacct gctgggaagg gagcgtaacc gggaaggggc aacggtgctt
     1621 cagggtaatc cggaagaagt ccgggaactc atcgatgcca cgccatttgc gaagaaatac
     1681 acgtccggtg ttctgtcgtt cgcggagaag gagctgccgc cgggaggacg tgaaaaagtg
     1741 atggcgagct ttgagcgtgt actgatgccc ggtctcgaaa aaaatcagta cagcatcctg
     1801 tgggtggatg caccaggaca agggacggct tgagctgaat tttgtcattc cgaacatgga
     1861 gctacagacc ggaaaacgcc tccagccgta ctacgaccgc gcagacaggc ctagaattga
     1921 tgcctggcag acgctggtta atcactatta cgggctgcat gacccgaacg ccccggagaa
     1981 ccgcaggacg ctgacactcc ctgataatct gccggaaacg aaacaggcga ttgctgaggg
     2041 cgttacacgc ggtttaccat gccggagaga taaaaggccg tcaggatgtg attcaggctc
     2101 tcacagaggc agggattgac gtggttaggg tgacgcgaag cagtatcagc attgcagacc
     2161 cgaacggcgg gaagaatatc agactgaaag gagcgtttta tgagcaatct tttacagacg
     2221 ggtgcggagt tcgagaaaaa gctgaaagag agagccgaat ctacagagaa aatgctgaac
     2281 gacgagttca ggaagctcga agaatctgta aacagggaac tgacctcaaa cgaatcttta
     2341 atcaggaacg ctatcaacga ccatacatca gccctgaaag aacttctgga gaggtatcag
     2401 aaaacgaccg tcgatacgat ggacgcacac tggaaaaccg tactgaaaat gagcgtgaaa
     2461 cgctggctgt ggctgattat cgtctcagta ctgatgtttg ccacgaccgg gagcctgctg
     2521 tggtaccagg gcatgaagat aaacgcgaac atgaatattc tccgcgagta gaaagagagt
     2581 ctggagaagc tgaacgcgaa gacatggggc gtgcgttatc acgaggacag caacggacgt
     2641 tttctggtgt tgcccgaggg gatgaaagcc gaaacgaact ggacgatgga aaacgggaaa
     2701 cagaatgccg taaggctggt acaggagtaa cagagcatga cggagctgga aagacatttg
     2761 ctgaacgcat tagaacaact gcaacaggac tatatgcagc ggctgaacga atgggagagc
     2821 gccttcgtgg aattgcagaa aatgttttcg cttacgcaac gggacaacgc gatgctgaac
     2881 gagcgggtca tgcagttgag tcagcaggtg cagcacttga gcgagcagac agaacgcttg
     2941 agccagttat acagcgagaa caggagataa gggacgaacg gctgatacag gaatgcgaac
     3001 atgcgttatc cctggagcgt gagcaccagc tggaaataca ggaacggaca ctggatggcc
     3061 cttcgctggg atggtaatca gagacaggct aaaatgttgt cagcgcgttt ccggtgcatt
     3121 ttttgggata atggttggtt cgggggcacc gggaacgcgc ttcagtcaga ccttgctgaa
     3181 aacataggaa cgcgttaaaa cgcatacggg cgcgattctg gtgtgtcttt tgttgctgtg
     3241 ttttgcctct attgccagat cgcgctcatt cccctgaact tcttttttcc cgctcaggtc
     3301 cccgtcttct tcgcttccga aagcatgaaa aaaatccgtt tcagctgtct ctggctggca
     3361 attcctgcgt gattcacatg gctgcatagc tatgcatgtg gttaaaattt accgggtgcg
     3421 atcgcggcag tttttcgggt ggtttgttgc catttttacc tgtctgctgc cgtgatcgcg
     3481 ccgaacgcgt ttcagcggtg cgtacaatta agatattacg ttaaattgtg agggaatggt
     3541 ccccatatac tgactttatc atagggatag ggttggtagt gggtcgattg ggtttcggca
     3601 ttgtttccgt taatgccaat tgcatttttg tatttgatgc aacagaaggc gtgagattct
     3661 cctgggcctg gaaaggataa gtgtttagaa ttgatggcaa aatatcttcc atccgatttt
     3721 tgaagtagtt gttgtttggc tgtctcccaa agggtgttgt tacaaacttc ttgaatttgt
     3781 agtttttgca ggactcttat tactgcgtta tcatgttcca attctttagg ggagtgtatt
     3841 attctctctt ttaataagct gtttaccaca gggtttagtt gttgtctaaa gcacgcagat
     3901 aatgccattg ctacacaatt ttgttcccga ggaagcatgt ttggcggggt atgccgtgtt
     3961 tgatgtgata tgtctattct catagaaggt ctctggctta tagcatcaac aaatacggct
     4021 ataaaacaaa attttcatag agtgattcaa gatcttcttg agatcctttt ttgcggcgcg
     4081 taatctgctg cctgcaaac
//
LOCUS       DAEEUE010000205         3777 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.205, whole genome shotgun sequence.
ACCESSION   DAEEUE010000205 DAEEUE010000000
VERSION     DAEEUE010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.205"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            32..493
                     /locus_tag="KLK84_004085"
     CDS             32..493
                     /locus_tag="KLK84_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HBE2011189.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(534..1004)
                     /locus_tag="KLK84_004086"
     CDS             complement(534..1004)
                     /locus_tag="KLK84_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HBE2011190.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(1051..1770)
                     /gene="yehT"
                     /locus_tag="KLK84_004087"
     CDS             complement(1051..1770)
                     /gene="yehT"
                     /locus_tag="KLK84_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HBE2011191.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1767..3452)
                     /gene="btsS"
                     /locus_tag="KLK84_004088"
     CDS             complement(1767..3452)
                     /gene="btsS"
                     /locus_tag="KLK84_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HBE2011192.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          993 a          975 c          858 g          951 t
ORIGIN      
        1 agtttcttga ttaaataaaa tggagtttta catgaaggct ttcaataagc tgttttccct
       61 cgttgttgca tctgttctgg ttttctctct tgctggctgc ggtgacaaag aagaatcgaa
      121 gaaattcagc gccaatctga acggcactga aattgccatt acctatgtct acaaaggtga
      181 caaggtgctt aagcaatctt ctgaaaccaa aattcaattt gcctccattg gtgcaaccac
      241 caaagaagat gctgccaaga cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg
      301 tgttgaagaa aaatcaacct ataccgatac ctacgcgcag gaaaacgtga ctatcgatat
      361 ggaaaaagtg gattttaaag ccctgcaggg tatttcagga atcaacgttt ctgctgaaga
      421 tgccaaaaaa ggtatcacta tggcgcaaat ggaactggtg atgaaagccg ctggttttaa
      481 agaagtgaaa taatctgtcg gcggccatgt ttcgcatggc cgccataccc gtcttagctt
      541 ttcttcacat gctgacgcgc tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg
      601 cattcgcgga agtttttatg atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa
      661 acgcggaact gctgttcggt gaggatagcc agaatgtcat cggttttcag cgaaaacgcg
      721 atgcgtaatt ttttcagcac gatgttgtta ttaatgcgac gttccggctc cagtgccgga
      781 gcagactcat ccttgccgcg tttttcataa atcaggccat tgaggaatga cgacaaaaca
      841 atgtccggac aacgctgaaa accctcttcg tcttctttac gtagccagac ggcgatctgt
      901 tccgcggtgg cttcgacatt atccagcgcc agaatacgca ccaggtcatt attattggct
      961 ttcaaaatgt agcgcacgct gcgcagaata tcgttactta tcatgaggcc ttcaggtgtt
     1021 gatgaggcaa aaagccattt tagcagtctt ttacaggcca atcgcctctt ttaagctttt
     1081 cagataacgg cggctgaccg gcacggttaa gccattacgc aaaatcaact cggcctggcc
     1141 gttatcttcc agacgaatct cctgtaaatg cgcgaggtta accagatact gacgatggca
     1201 gcgcagtagt ggtgtacgac tttccaaggt acgtaatgtc aattcggtaa agccctcttt
     1261 cccttcgtgg ctggtaacgt agacaccgct catccgactg ctgacaaatg ccacatcttt
     1321 catttgcagc aaataaatcc gactatgccc cgtacaaggg ataaatttca gcgcctgttg
     1381 attttccggt aacagcgaaa catcctgctt gctgcgctcc tgacgcaatc gcgccagcgt
     1441 tttctccagt cgcgcttcat caattggctt cagcagataa tcaaaggcat gttcttcaaa
     1501 ggctttaata gcgtattcgt caaacgcagt gagaaaaaca atatacgggc gatgttccgg
     1561 gtcaagcatc cccaccattt ccagaccact gatgcgcggc atctggatat cgagaaacag
     1621 cacatccggg cgcagtttat gcaccgcgcc gatcccttcc acggcgtttg aacactctcc
     1681 aacgatttca atatcgctct gctcctgcaa aaatatacgc aggttctccc gtgctaacgg
     1741 ttcatcatcg acaattaaga ctttaatcat gcctcgtccc tccatggtag tcgtaacgtt
     1801 attcgggtgt aactatcagg ctcacaggcg acgcttattc catagtcatc gccaaaccgt
     1861 tcacgtaaac gcttatccac cagattcatc cccagcccac tggcattggt taccggttga
     1921 tacaaaccgg cattgtcttc gatctccagc atcaaatgtt gcccctcacg tcgggcgctg
     1981 attgccactc gccctgtatc cagcagttgt gatgtcccat gtttaatggc gttttccact
     2041 atcggttgca gggtaaacgc gggcaattgc tgctgggata attcttgcgg aatagcaatg
     2101 ttgacctgca accgcgactg gaagcgcgcc ttttcaattt gcagataagc gttcacatgt
     2161 tcaatttcgt cggcgagagt aacaaactcc gaaggccgct ttaagttttt gcggaaaaaa
     2221 gtggaaagat actgcaccag ctggctggcc tgttcgctgt cgcggcggat caccgcttta
     2281 atggtgttaa gcgcattaaa caaaaaatga ggattcacct gggcgtgaag cagtttgatt
     2341 tctgactggg tgagcatcgc tttttgccgc tcatattgcc cggcaagaat ctgcgccgaa
     2401 agcagttgcg caatcccctc gcccagcgtg cggtttattg aactgaataa ccggtttttg
     2461 gcttcataca atttgatggt gcccatcacg cgctgatttt cgccacgcaa cggaattacc
     2521 agcgtcgacc ccagtttgca ttgcggatgc aaagagcaac gataaggtac ttcgttgcca
     2581 tcagcgtaga ccacttcacc ggtttcaatc gcttttaagg tgtaagtcga agaaatcggt
     2641 ttgccgggta aatggtggtc gtcaccaatt ccggtaaagg ccagcaattt ctctcgatcg
     2701 gtaatcgcga ctgcaccaat atccagctcc tgatacagta cctgagccac tttcatgctg
     2761 ttcacttcgt taaacccctg tcgcaaaatg ccttccgtcg aggctgccac tttcagcgca
     2821 gtggcagaaa aagccgaagt gtatttttca aacatcgcgc gtttatcgag caatatacgc
     2881 ataaacagcg ccgcgccgac ggtattggtg accatcattg gcgcagcaat attactcacc
     2941 agacgcaccg catcttcata aggtcgggcg atcgcaagga tgatcagcat ttgcaccatt
     3001 tcagcgacga acgtgacggc accggcggta atggggttaa agactttatc agtgcgcccg
     3061 cgacggatca ggatgctgtg taccaggccg ccgagcaatc cttcaacgat ggttgagatc
     3121 atgcaactta gcgcggtcat gccccccatc gaatatcgat gtaagccgcc ggtcagacca
     3181 accagcccac cgacgaccgg accgccgagt aagccgccca ttaccgcgcc tatcgcacgg
     3241 gtattggcaa tagaatcgtc aatgtgcaac ccaaaccagg tgcccatgat gcagaagatg
     3301 gaaaagacga tgtagcagag aaatttatgc ggcagacgaa ccgtgacctg cattaacggt
     3361 atgaataatg gcgttttact cattaaccac gcaatgacta aaaaaacgca catctgctga
     3421 agcagcagca acaccagatt aaaatcgtac atacccgcaa accacacttc cctttaaaac
     3481 gcgtaacata cattgcctgc gtttaacttt ctttgaactc ttgcagaaaa atgagaattc
     3541 gtgagtacga tcactcaaaa tcgcctggca aaaataaaat caccctatag atgtaatgcc
     3601 gatcagttaa ggatcagttg accgatccag tggctgtgta agaatccgga aacgctcact
     3661 tgtttccgga tttttttatg cacattggac aggctcttga tctggtatcc cgttacgatt
     3721 ctctgcgtaa cccactgact tctctggggg attacctcga ccccgaactc atctctc
//
LOCUS       DAEEUE010000206         3663 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.206, whole genome shotgun sequence.
ACCESSION   DAEEUE010000206 DAEEUE010000000
VERSION     DAEEUE010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.206"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            225..1453
                     /locus_tag="KLK84_004089"
                     /pseudo
     CDS             225..1453
                     /locus_tag="KLK84_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            1828..2016
                     /locus_tag="KLK84_004090"
     CDS             1828..2016
                     /locus_tag="KLK84_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBE2011193.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(2066..2395)
                     /locus_tag="KLK84_004091"
     CDS             complement(2066..2395)
                     /locus_tag="KLK84_004091"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011194.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(2520..2699)
                     /locus_tag="KLK84_004092"
     CDS             complement(2520..2699)
                     /locus_tag="KLK84_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011195.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            2829..3029
                     /locus_tag="KLK84_004093"
     CDS             2829..3029
                     /locus_tag="KLK84_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HBE2011196.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            3019..3240
                     /locus_tag="KLK84_004094"
     CDS             3019..3240
                     /locus_tag="KLK84_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011197.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            3233..3453
                     /locus_tag="KLK84_004095"
                     /pseudo
     CDS             3233..3453
                     /locus_tag="KLK84_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3455..>3663
                     /locus_tag="KLK84_004096"
     CDS             3455..>3663
                     /locus_tag="KLK84_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011198.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDR"
BASE COUNT         1022 a          829 c          890 g          922 t
ORIGIN      
        1 tgcaaccgat tataacggat gcttaacgta atgcgtgaag tatggacata ttttatcaat
       61 tagtgttttt tgccgtttca taatgtttca gattgttcat tatgtttttg attataaaca
      121 gatttgtgat tttccttgtt ccagcttgtt tcacgttgta gccctttgtg caacgatcgg
      181 tgtagttaat ggtgtagtta attgtgatga ccggaatcaa aattatgagc agagcactta
      241 acaaactgag cgatacacag ctgaggaaaa tcaacggcac acccgcccaa aaaacagcct
      301 ttcttaatga cggtggaaac ctgagcgtca ggcattcaac cagtggcctt ttaacctggt
      361 atttcactta cagggccgga acgggaaggg gggcaccacc ggaacgcatt aagctgggaa
      421 attatcctga tctgaacctg aaatcagcca gggaaaaagc cgcccagtgt cgcgcatggc
      481 tggcagaggg gaaaatccac gtcatgagct taattacacc gtacaggaag cgttaaagcc
      541 ggtaacggtt ggcgatgcgc tcacctactg gcttgagtcg tacgcaaagg aaaaccgcgt
      601 ggattatgcc gccctgaaaa agcgccttaa taatcacgta atacagcaca ttggtgctat
      661 gccgctggat aaatgcgagc tacggcactg gctggcctgt tttgaccagg tggcaaagcg
      721 aacgcctgtt actgccggat tcttgctaca gacgtgcaaa caggcgctta agttctgccg
      781 gaggcggcgc tatgcaatca gcaacgttct tgatgatatg agtgtggcgg acgttgggaa
      841 aaaaccggat ataagcgagc gtgtcttaag caccaaagaa ctgggcgaat tattgcaggc
      901 actggacaaa aaaatattct ccccctacta catcgcgtta atccgcctcc tgattgtgtt
      961 cggatgccgg acggtagaac tgaggttatc ggagatcagc gagtgggatt ttaccgaaat
     1021 gctctggacc gtgccgaaag aacacagcaa aacgaaggtg gcaatattcc ggcccatacc
     1081 ggaagcaata ctgccgttcg tcacgcagct ggtggagcag aacaggcaca cgggcttatt
     1141 gctgggggaa gtgaaacagg aaacaagcgt gtcgcagtac ggcagattag cgcacaggag
     1201 gctaaatcac cctcactggt cactacatga catccggcgc acctttacaa cgatgctgaa
     1261 cgatttaggc gtggatcctc acgtcgtgga gcagcttaca ggccaccaga tgccaggaat
     1321 gcagcgagtt tataatcatt cccgttatct ggatgctaaa cgcaatgcgc tggatatgtg
     1381 gacggagcgg ttagggatac tggcgggaac acatgaaaac gtaaccacgc taccagtagc
     1441 cagaagaaaa taattttttt cgtgtttttt cagtatgcgc atactggata tgcgaacaga
     1501 tacaacgtgc aacaatgagc aacaaagcgg aacaactacg aacaagaggt gaaaaagtgt
     1561 acagatttat aaatagctga tttttaatgt gttgctgatt ttttatacac tagcgaatca
     1621 ctctttaaat agcgagaaaa aaggcatgaa acaatatttt atgcctttgt attaacatga
     1681 atttaaagga ttttatcctg attattcctc tttctgtaaa ccatgcggcc tcaacatttg
     1741 ccaactctga atcatcctga cacaatcaga cacatcccaa cgcaatagaa cacgaaataa
     1801 ctctttaaga aacgaaaggg ggcaatagtg ttaagcactg atcggtttat acgtgaaaaa
     1861 gaatgcgaaa agctaacagg ccttagccgc tcatgccgct accgcctgga aaaggccgga
     1921 caattcccat cacgtcgtaa acttggcggt cgttccgttg gctggtcttt atccgaggtt
     1981 ctggcctgga aggatagctg caaggcagtt cattaatcac gctggcggca cgcagccgcc
     2041 acacatcaat catctgaaca cagagctata accatgaaga ttgaatatac gccagaacgt
     2101 gggcggggat tcgttcgccc tggtgaaact ggaaaaccac aaaattgggg tttttcaggc
     2161 ataaaaaaag cggccccgaa atggagccgc ctttctgaac agataacccg ctgcgccgtt
     2221 tgtgtgtgtg atcccaaaca taagcacggg gatgatagcc gctatcaggc tggtgggcaa
     2281 tgcaatcagt ctggttcagt gcgttgccat aactgcgaag ttctctcctt tgatgagtat
     2341 tctttaaggt acttctcaag ggcaaaagaa cacggcgcga atcgttctga ttcatgctct
     2401 ttctttctgc gtcgtctttt tcgtgcaggt gatgtttttt cggttgattc ttcgttggtc
     2461 attgtgtgcg cctgtaaagc aatgcgccgt agtacctcac accacggcgc tgatagtttt
     2521 tatcctttgg gttctatgcc gcgttgctgt aattctttgc ggattattcg ctttatccag
     2581 gcagcaagag aatcatcgcc gtcctgttgt tgtgcctttt ccatcttttc ccgtaagtca
     2641 ggatctaaac ggaactggaa cggaggatta ccacgccttt cgtttttgtg tgttgacacg
     2701 ttaattacac ctcaggtaat gtatttatgt gtagtgacac attacacaca atcaacacaa
     2761 aaagcaaagc cccgcactgt aggagcagta acggggcttc taaccaacaa cgtaaactag
     2821 gagccgttat ggttgccgta aatcatatac cacaccttgt acacacacaa acggcctttg
     2881 tgtggcgttt tctggcactg agtgccggag aatcccaaat catccacgta accgcttgga
     2941 cggaacgcga agcgcgtagc cgttgcccgt ccggttgtgt tgctgtattc gccgcccgta
     3001 ttcgtcaggg ggaaacctat gcacaataaa accacaccga acgcagcagc cgccgcactc
     3061 actacgctga tgcacgcgct tattgatatt gaatgcacgg cagagcttgc gcagggagaa
     3121 gaacagaaag accggacaca gttcgccctg gaatgtatcc gatacatcgc aacgcggtca
     3181 ctgaatgacg ctaaaaatat tcttgttgcc gattgtgaaa atgggggggg ttatgcgtga
     3241 tgatcgtttt aattccctga aacaggaatt ttccggcgtt cctgatgatg cggctgatgc
     3301 gctttcgtca atgccagaac ttattagagc ggcttttttc ttactttcca cgagagaata
     3361 taaatcaacg ggcttgatgt actgaatatc gccgccgatt atgctgattt cgtgacagag
     3421 gttattttaa gaaaaacgac ggacggggat taatatgcgt gatatttacc acgaaacaat
     3481 agaccgcgca tttcttgcac tttctcacag tgaaaacatg ctggaaatat tgcgcatatg
     3541 gcttgaaaca cttggcgaca atgaacgcga caaacaaaaa tcaagaattg ccacggcatt
     3601 aataacgctt cttgagcctg taataatgga actgcaagaa atagatctat tgcacgacag
     3661 ata
//
LOCUS       DAEEUE010000207         3586 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.207, whole genome shotgun sequence.
ACCESSION   DAEEUE010000207 DAEEUE010000000
VERSION     DAEEUE010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.207"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..380
                     /locus_tag="KLK84_004097"
     CDS             <1..380
                     /locus_tag="KLK84_004097"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HBE2011199.1"
                     /translation="LPASRVYPGSSRQAYLANRALHELRKNRTLPVDEAWVDDFVREA
                     FGRMKKEYVCKDSEMATEGATDLWEGSLIDQLITEVIQSSHSRAAALAVDSAAAKLMQ
                     NAENVSEYLSLRHQGLQQSIQSL"
     gene            438..1412
                     /locus_tag="KLK84_004098"
     CDS             438..1412
                     /locus_tag="KLK84_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011200.1"
                     /translation="MTADVRMAMEEIDARTRELLTGVCTSLEEELNDYFRSGKRKEQQ
                     MLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRREALELMTQIE
                     STVTSLHREAEAQFRPELEKIVRGIETGFRGTALYATENIAGRINARLEDEGFTVKIS
                     FPAVSQLQTRLAVKTNLSALMEERTETVTRRCRQDGVWGTLCRWANTSDWGWKEYSVD
                     VSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCTFREKVEQLRN
                     TLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML"
     gene            1409..2314
                     /locus_tag="KLK84_004099"
     CDS             1409..2314
                     /locus_tag="KLK84_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HBE2011201.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLSGEGTARQNAINASLAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHQKLNHLEEKLHRVDLVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            2311..3381
                     /locus_tag="KLK84_004100"
     CDS             2311..3381
                     /locus_tag="KLK84_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBE2011202.1"
                     /translation="MSIEIKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRSVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISIINGFDNPMTQPLLSDGPLTALMDHYLDTDALAGGLPLYVSLYP
                     TEGGMQDIIDCIRAELGSGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVSHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGGNSGGLLSFTSAHTDAWRQQGYEDTMLTMEHIRKPLAARQALSRSETVL
                     QKSLEITEEADLALRSAMARIK"
BASE COUNT          904 a          842 c         1036 g          804 t
ORIGIN      
        1 tgctgcccgc ctcccgcgtt tatccaggct catcacgtca ggcgtacctg gctaaccgtg
       61 cgcttcatga gttacggaaa aacagaaccc ttcctgttga tgaagcctgg gtcgatgatt
      121 ttgtcaggga agccttcggt cgcatgaaaa aagaatacgt ctgtaaagac agtgaaatgg
      181 caaccgaagg ggcgacagac ctgtgggaag gctcacttat cgatcaactg ataacggaag
      241 tcatacagag ctcccattcc agagcggcgg cactggcggt tgattctgcc gccgcaaaac
      301 tgatgcagaa tgcggaaaat gtcagcgaat atctgtcatt acgccatcag gggctacagc
      361 aaagcattca gtcactgtag tcgcatatca ccagcctgct tgcggatatc cgggaaatcg
      421 cggactgtca ggagcaggtg accgctgatg tcagaatggc catggaggag atcgatgcca
      481 gaacccggga attactgacg ggggtctgca cctcactgga agaggagctg aatgactatt
      541 tcagaagcgg taaacgcaaa gaacagcaaa tgctggagga agaaaattca gcgcagcccc
      601 gggagagaaa cgcattcgct ttctttcacg atattttcgg aaccggaaat cagcatgaca
      661 ggatgcggga ctttgatccg gacagccccg aaataaaatt cagtgatcgc agggaagccc
      721 ttgaactgat gacgcaaatc gaatcgaccg tgaccagcct gcaccgtgag gctgaagcac
      781 agttccgtcc tgaactggaa aaaatcgtca ggggtattga aacaggtttt cgtggtacgg
      841 ccctgtacgc cacagaaaat attgccggac gcatcaatgc ccgtctggag gatgaaggtt
      901 tcactgtaaa aatcagtttt ccggcagtca gccagttaca gacccggctc gcagtaaaaa
      961 caaatctgag cgcgcttatg gaggaaagaa cggagacagt aacccgtcgc tgtcgacagg
     1021 atggtgtctg gggtacgctt tgtcgatggg caaacaccag tgactggggc tggaaagagt
     1081 acagtgtgga tgtgagccgc agcgtgatca atatcaacaa ggtcagagag gaagtgatgt
     1141 cactgacccg ggcatatttc ggggagctgc aggcatccat tgagcaggat attaaccagc
     1201 ccgtccgcca ggaaatcgat gcctttttct gtacattcag ggagaaagtt gaacagctgc
     1261 gtaacacgct gattcagagc tctgaagatc ataaacgcga tcagcaggcg caggaacggc
     1321 tgaccgggcg tcttcaggcg ttaaatgaaa gggttcctga gctcattact gacagtaagg
     1381 cgttaaggga agaactggag acaatgctgt gacttcacca tttattcaac aaattgctga
     1441 taaccgggta tgtcaggtac tcacctgtct tcctgaaaaa tttgtggttg atttcgcaaa
     1501 cggtattgat gttgcacagg agcatatccg tacggccgga gagcgcacgt tcttccggcg
     1561 gttaaaagag gggctgagcg gcgaaggtac tgcacggcag aatgccatca atgcttcgct
     1621 tgcacagggc gttgaggcgt ccctgcgctg gctgacggag ctgacaacgt cacttgccac
     1681 cacaaactac gcgattaccc gggtaaacga cagggtcagt tcactggtca gtgatactgc
     1741 caggctggcg cattattccg cagatacgcg ggagcagtta ctcaccctgg ccgatcaggt
     1801 tcaccagaaa ctgaatcatc ttgaagaaaa actccaccgt gttgacctgg ttcagcgggc
     1861 gcagttacat cttgagcaga tattctcctg gtggagcgcc gggcgatatg cgtcattttc
     1921 ccctgccgga cgttgttatg tggcacttga agagcttcgc tggggcgcgt ttggtgatgt
     1981 gatacgtcag ggcgaaacag gccaggttaa ccagctactg gatattctca gacataaagc
     2041 attaacgcag atggcacagg agagtggcgg tagtgcaacc gtgcgtctga acactctgga
     2101 ctggcttggt ggtcagggcc gggagcaggc agataacgag tggcatgacg cgattaactg
     2161 gctgggggac tggtgcagtg aagagcagca tccggtgatc tggtcaacca cgcaggctgc
     2221 agaacactta ccggttcgca tgccccgtct ctgctctgca gaacgcctct ctgaaagtat
     2281 ggtcgatgaa atatttcaga agggggctgc atgagtatag aaataaaaac agggctggtg
     2341 ctgtccggcg gtggggcggt gggcgcttat caggcgggag tggttaaggc actggcagag
     2401 tgtggtacac agatcagcat ggtttcaggg gccagcattg gcgcattcaa tggtgccatt
     2461 atcgcggcct ctcccgatct gtcagaagct gccgtacgcc tggaggcgct ctgggatcat
     2521 cttgggaata atcaggtgct gtcggtaaac cgatcggttt acttttcatt gctgaaaaaa
     2581 ttgttccagg caatgaacct ctgccagatc cccggacgtg caggagcact gcttacgaca
     2641 cttcttcgcc atatatcgat aatcaacggc ttcgacaatc cgatgactca gccgttgttg
     2701 tcagatgggc ccctgacagc gttgatggat cattatcttg atacagatgc tctggcaggc
     2761 gggctaccgc tgtatgtgtc gctgtacccc acagaagggg gcatgcagga tattattgac
     2821 tgcattcgtg ctgaactggg ttccggaacc acgaaaaacg ccgtttttca gcatatccag
     2881 agtctgcccc gcggacagca gaaagaggct ctgcttgcgt cagccgcact gcccctgttg
     2941 ttccgtcccc gtgaggttca ggggacaatg ttcggtgatg gtggtatggg aggatggcga
     3001 aatatgcagg gaaatacccc tgtaacgcct ctggtcgatg ccggatgcaa tatggtgatt
     3061 gtgtcgcatc tgagtgacgg ttctttatgg gatcgccagg cttttccgga caccacaatc
     3121 cttgagatcc gtccccggaa aaggctgaaa tatgcaggtg atggtggcaa cagtggcggt
     3181 ctgctcagtt tcacgtcggc acataccgac gcctggcgtc agcagggcta cgaggacacg
     3241 atgctgacga tggagcatat ccggaaaccg ctggcagcac gtcaggcact gtcccggtca
     3301 gaaacggtgt tgcagaaaag tctggagata acggaagagg cagatttggc actgagaagc
     3361 gcgatggccc ggattaaata aagacgcccc aaagaaacgc caccgcctgt ggtggtgttt
     3421 ttcctgtctg gtgttgttga aaatattttc agatatatat tacctgggtg agatttaaga
     3481 ttaacgcatt tattaatgtc ataaggagag ttatcatgaa attcatcgta aaagcactta
     3541 ggtgatgctg ccaacttact gatttagtgt atgatggtgt ttttga
//
LOCUS       DAEEUE010000208         3558 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.208, whole genome shotgun sequence.
ACCESSION   DAEEUE010000208 DAEEUE010000000
VERSION     DAEEUE010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.208"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            84..290
                     /locus_tag="KLK84_004101"
     CDS             84..290
                     /locus_tag="KLK84_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011203.1"
                     /translation="MKSVTIEAKTFAEMLGITEGELIFAIKKTGTFKNKTIPQPHEPH
                     KSNNRFLYSDVMRFIESLKDKENR"
     gene            complement(301..573)
                     /locus_tag="KLK84_004102"
                     /pseudo
     CDS             complement(301..573)
                     /locus_tag="KLK84_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            702..2105
                     /locus_tag="KLK84_004103"
     CDS             702..2105
                     /locus_tag="KLK84_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001157239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBE2011204.1"
                     /translation="MLEKAEAEEQLRQENDKKLLGQVLEIYDQKYVAELLRKVGKNEW
                     SRETLNRWINGKCSPKTLTLAEEELLRKMLPEAPAHHPDYAFRFIDLFAGIGGIRKGF
                     ETIGGQCVFTSEWNKEAVRTYKANWFNDAQEHTFNLDIREVTLSDKPEVPENDAYAYI
                     NEHVPDHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFECEAQGTLFFDVARIIRAKKPA
                     IFVLENVKNLKSHDKGKTFKVIMDTLDELGYEVADAAEMGKNDPKVIDGKHFLPQHRE
                     RIVLVGFRRDLNIHQGFTLRDISRFYPEQRPSFGELLEPVVDSKYILTPKLWEYLYNY
                     AKKHAAKGNGFGFGLVNPENKESIARTLSARYHKDGSEILIDRGWDMATGETDFANEE
                     NQAHRPRRLTPRECARLMGFEKVDGRPFRIPVSDTQSYRQFGNSVVVPVFEAVAKLLE
                     PYILKAVNADSCKVERI"
     gene            complement(2139..3353)
                     /locus_tag="KLK84_004104"
     CDS             complement(2139..3353)
                     /locus_tag="KLK84_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_143212653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II site-specific deoxyribonuclease"
                     /protein_id="HBE2011205.1"
                     /translation="MLMSVFHNWLLEIACENYFVYIKRLSANDTGATGGHQVGLYIPS
                     GIVEKLFPSINHTRELNPSVFLTAHVSSHDCPDSEARAIYYNSRHFGKTRNEKRITRW
                     GRGSPLQDPENTGALTLLAFKLDEQGGDCKEVNIWVCASTDEEDVIETAIGEVIPGAL
                     ISGPAGQILGGLSLQQAPVNHKYILPEDWHLRFPSGSEIIQYAASHYVKNSLDPDEQL
                     LDRRRVEYDIFLLVEELHVLDIIRKGFGSVDEFIALANSVSNRRKSRAGKSLELHLEH
                     LFIEHGLRHFATQAITEGNKKPDFLFPSAGAYHDTEFPVENLRMLAVKTTCKDRWRQI
                     LNEADKIHQVHLFTLQEGVSLAQYREMRESGVRLVVPSSLHKKYPEAVRAELMTLGAF
                     IAELTGLYADIP"
     gene            3409..3474
                     /locus_tag="KLK84_004105"
                     /pseudo
     CDS             3409..3474
                     /locus_tag="KLK84_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
BASE COUNT          984 a          844 c          846 g          884 t
ORIGIN      
        1 gaagcgtacc gcagcaacac tagaaacgag caccattgtt aaagatgccg gtaaagaaaa
       61 tcgataccat gagagttaat taaatgaagt cagtaactat agaagcaaaa acatttgctg
      121 aaatgttagg aataacagaa ggtgagttaa tctttgccat taagaaaact ggcacattca
      181 aaaacaagac catcccacaa cctcatgagc cacataaatc aaataataga tttttatatt
      241 cagacgtaat gaggtttata gaatcactaa aagacaaaga gaaccggtaa tgactccaac
      301 ttactgatag tgttttatgt tcagataatg cccgatgacc ttgtcatgca gctccaccga
      361 ttttgagaac gacagtgact tccgtcccag ccttgccaga tgttgtctca gattcaggtt
      421 atgtcgctca atgcgctgag tgtaacgctt gctgataacg tgcagctttc ccttcaggcg
      481 gggttcatac agcggccagc catccgtcat ccataccacg acctcaaagg ccgacagcag
      541 gcccagaaga cgctccagcg tggccaacgt gcgttaccta acaataaacc tgtttaaata
      601 tccagataaa aacattcaat ctgggtcaaa tgagtgatac agtttcaccc ataagaccca
      661 atggaggcaa tatgtctgaa tttgaattac tggcgcagga tctgcttgag aaagcagaag
      721 cggaagaaca actgcgacag gaaaatgata aaaagctgct cgggcaggtg ctggaaatct
      781 atgaccagaa gtacgtggct gaactgctta gaaaagttgg taaaaatgag tggagtcgcg
      841 agactcttaa tcgctggatt aatggtaagt gctcacctaa gacgctgacg ttagccgaag
      901 aggaacttct acgaaaaatg cttccggaag cgcctgcaca tcaccctgac tatgccttcc
      961 ggtttattga cctgtttgct gggattggag gtatacggaa gggcttcgaa accatcggtg
     1021 gccagtgcgt ttttaccagt gaatggaata aagaggctgt gcgcacatat aaagctaact
     1081 ggtttaacga tgctcaggaa cacactttca atctcgatat tcgtgaagtc acgctcagtg
     1141 ataaacctga agtacctgaa aacgatgcct atgcttacat taatgagcat gtgccggatc
     1201 atgatgtact tctagcaggt ttcccctgtc aaccgtttag ccttgcgggc gtaagcaaga
     1261 aaaactcgct cgggcgcgcg catggtttcg aatgtgaggc tcagggaacg cttttcttcg
     1321 atgtggcgcg tattatccgc gcaaaaaaac ctgccatctt tgttcttgaa aacgttaaaa
     1381 acctgaagag ccatgacaag ggtaaaacct ttaaagtcat catggatacc ctcgacgaac
     1441 tgggctatga agttgcggat gcagctgaga tgggcaaaaa cgatcctaaa gttatcgacg
     1501 gaaagcactt tttacctcag caccgagaac gtatcgtttt ggtcggtttc cgccgtgatc
     1561 tgaacattca ccagggcttt accctgcgcg atattagtcg tttttatccg gaacagcgtc
     1621 cgtcatttgg cgaactgctg gaacccgtgg ttgacagcaa atatatactg acgccgaaac
     1681 tctgggagta tctctataac tacgccaaaa agcacgcagc taagggtaac ggattcggtt
     1741 ttggcctcgt taatcctgaa aataaagaaa gcattgcccg tacgctttct gctcgctatc
     1801 acaaagacgg gtctgaaatt ctgatagacc gtggctggga tatggccaca ggtgaaacag
     1861 acttcgcgaa cgaagaaaat caggcgcatc ggccccgcag gctgactccg cgagagtgcg
     1921 cgcgccttat gggttttgaa aaagtagatg gcaggccttt tcgcattcct gtgtcagaca
     1981 ctcagtcgta caggcagttc ggtaactccg tagtggtgcc cgtgtttgaa gccgtagcca
     2041 aactgcttga accttatatc ctgaaagcgg ttaatgccga ttcgtgcaag gttgaacgaa
     2101 tctgatcgct cctcccggta tttatgccgg gagataatct atggaatatc tgcgtaaagc
     2161 cctgtcagct cagcaataaa cgcacctagc gtcattagct cagctctcac cgcctccggg
     2221 tattttttgt gcagagatga tggcacgacc aatctgacac ccgactcccg catctcccga
     2281 tattgagcca gagaaactcc ctcctggagt gtaaacagat gcacctgatg aattttatcg
     2341 gcctcattca gtatctgacg ccagcgatcc ttacaggtag tcttgactgc cagcatgcgc
     2401 agattttcta cgggaaactc agtatcgtgg taagcccctg cggaagggaa aaggaaatcg
     2461 ggttttttat taccttctgt gatggcctgc gtcgcaaagt gtcgcaggcc gtgctcaatg
     2521 aatagatgct ccaggtgcag ttccagcgac ttcccggctc tggatttacg gcgattgctg
     2581 acagaattgg ccagcgcaat aaattcatcc acagagccaa atcctttccg gatgatatcc
     2641 agaacatgca gttcctcaac caatagaaat atgtcgtact ccacgcgccg gcggtcaaga
     2701 agttgctcat ccggatcaag ggaatttttc acataatggc tggctgcata ctgaataatt
     2761 tcacttcccg acggaaagcg caggtgccag tcttcaggta gaatatattt atgatttact
     2821 ggcgcttgct gtagagatag tccgcctaga atctgtcctg cggggccgga tataagcgct
     2881 ccgggtataa cttcaccaat agcggtctca atgacgtcct cttcatcagt gctggcgcat
     2941 acccaaatat ttacttcctt acagtccccc ccttgctcat caagcttgaa agccaggagc
     3001 gtcagagccc ctgtattttc aggatcctga agtgggctgc ctctacccca gcgggtaatc
     3061 cttttttcat tccgggtttt accaaaatga cggctgttat aataaattgc cctggcttcg
     3121 ctgtcagggc aatcatgcga tgacacatgt gcggtgagaa aaaccgaagg gttcagttca
     3181 cgggtatggt tgatagacgg aaagagtttt tcaacgatac ctgaagggat ataaagccct
     3241 acctggtgac cacctgttgc gccggtatcg ttggcggaaa ggcgtttgat gtagacgaag
     3301 taattctcac atgcgatctc aagtagccag ttgtggaaaa ccgacataag catcccctgt
     3361 taccctgaaa ctctactcac cattttttca tgattatata caaacagtgt cattttcaga
     3421 agacgactgc accagttgat tgggcgtaat ggctgttgtg cagccagctc ctgacagttc
     3481 aatatcagaa gtgatctgca ccaatctcga ctatgctcaa tactcgtgtg caccaaagcg
     3541 aggtgagcat ggcgacgg
//
LOCUS       DAEEUE010000209         3548 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.209, whole genome shotgun sequence.
ACCESSION   DAEEUE010000209 DAEEUE010000000
VERSION     DAEEUE010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.209"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..465
                     /gene="pgrR"
                     /locus_tag="KLK84_004106"
     CDS             <1..465
                     /gene="pgrR"
                     /locus_tag="KLK84_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HBE2011206.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            802..2415
                     /gene="mppA"
                     /locus_tag="KLK84_004107"
     CDS             802..2415
                     /gene="mppA"
                     /locus_tag="KLK84_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HBE2011207.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="KLK84_004108"
     CDS             complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="KLK84_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HBE2011208.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1006 a          773 c          819 g          950 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc
      361 actccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca
      421 ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata
      481 cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt
      541 tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc
      601 gaagcccgaa cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt
      661 tacactaaat ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt
      721 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta
      781 atgtcggtta gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg
      841 gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag
      901 aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc
      961 gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac
     1021 gaaaaagggg agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc
     1081 tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa
     1141 gattttgtct acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg
     1201 tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct
     1261 gatcagcttg gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg
     1321 ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac
     1381 gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt
     1441 cttaaagagc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat
     1501 aacgccaaaa cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc
     1561 actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat
     1621 cagaagctgt tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat
     1681 tattatgcct ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta
     1741 agtatgacga tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca
     1801 gcgtggcatt ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa
     1861 caaatgagtc aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat
     1921 ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa
     1981 attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa
     2041 aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc
     2101 gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg
     2161 cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca
     2221 tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc
     2281 atggagcaag caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg
     2341 tggctgaaag gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat
     2401 attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt
     2461 ctgatttagc gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg
     2521 aaggcaaagt ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc
     2581 tgtgcagcaa gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc
     2641 aatgacgagt cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc
     2701 gggtgatttt tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc
     2761 tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct
     2821 acgctgatcg acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta
     2881 attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa
     2941 cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa
     3001 ttactcagaa tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc
     3061 agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata
     3121 atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta
     3181 atctgctgtt tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca
     3241 atcagggacg tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt
     3301 tctataacat aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat
     3361 tgcaccgcat ggaataaaaa tccttttctt ttacgatttc cacggcgaaa ccaaaagctc
     3421 atcagaatca atgctgcgca gctaccgaaa ataatgacca gattaagcgc attatttgta
     3481 aacagttcag cgatcattgt tttatcaggc tcctccagat aattgtcgtc atgccggaaa
     3541 cccctggc
//
LOCUS       DAEEUE010000210         3477 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.210, whole genome shotgun sequence.
ACCESSION   DAEEUE010000210 DAEEUE010000000
VERSION     DAEEUE010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.210"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            116..1153
                     /gene="curA"
                     /locus_tag="KLK84_004109"
     CDS             116..1153
                     /gene="curA"
                     /locus_tag="KLK84_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HBE2011209.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1351..2016
                     /gene="mcbR"
                     /locus_tag="KLK84_004110"
     CDS             1351..2016
                     /gene="mcbR"
                     /locus_tag="KLK84_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HBE2011210.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMLILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(2287..3474)
                     /locus_tag="KLK84_004111"
     CDS             complement(2287..3474)
                     /locus_tag="KLK84_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HBE2011211.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPYCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
BASE COUNT          899 a          865 c          875 g          838 t
ORIGIN      
        1 gattcctcga tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca
       61 atcccgtgag cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg
      121 gcaacaaaag cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt
      181 tccggagaat ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag gacaggtgtt
      241 actgcgcaca gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc
      301 atcttattca ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt
      361 cgtggagtcg aatcatcctg attatcagcc tggcgactgg gtgctgggct acagtggatg
      421 gcaggactat gacatatcca gtggtgatga cctggtgaaa cttggcgatc atccgcaaaa
      481 tccatcgtgg tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct
      541 ggatatcggt cagcctaaag agggcgaaac gttggtggta gctgcggcga cagaaccagt
      601 gggggcgacg gtggggcaaa tcggcaaact taaaggttgc agagtggtgg gggtagccgg
      661 tggcgcggaa aaatgccgcc atgctaccga ggtgttaggc ttcgatgttt gccttgatca
      721 ccacgcggac gattttgccg aacaactggc gaaagcgtgc ccaaaaggta ttgatatcta
      781 ttatgaaaac gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc
      841 gcgcattccc gtttgtggct tagtgagcag ctataacgct acagagctac cacccggtcc
      901 ggatcgttta cccctgttga tggcaacggt gctgaaaaaa cgtattcgct tgcaagggtt
      961 tattatcgct caggattatg gtcaccgcat ccatgagttt cagaaggaga tggggcaatg
     1021 ggtgaaagag gataaaatcc actaccgcga agatattact gatggtttag agaatgcgcc
     1081 acagacgttt atcggcctgc tgaagggtaa aaacttcggc aaagtggtga tccgcgtcgc
     1141 gggtgatgat taaagtttca acggcggcgt aagccgccat aaaaatccag gcaaaaagct
     1201 tattttcagc tttaattaac taaccacgtc attgttctgc tgctaataac aacaaaggtg
     1261 ggtggtttat gattattcac tttaatacac taggtgaact ccttctgcca tgcaggcagg
     1321 gttggacaga aaacacgcga aggaattaaa atgcctggaa cgggaaaaat gaaacatgtc
     1381 agtttgactc tgcaggttga gaacgacctg aaacatcagc ttagtattgg cgcattaaaa
     1441 cctggcgcac gcctgattac taaaaatctg gcggagcaat taggtatgag tattacacct
     1501 gtgcgtgaag cattattacg tctggtttcg gtgaatgcgc tttctgtcgc acctgcacaa
     1561 gcatttacag taccggaagt gaggcaaagt cagttagacg aaatcaatcg gatccgctac
     1621 gaactggaat taatggcagt tgccctggct gttgaaaacc tctcctcgca agacatagca
     1681 gaacttcagg aattgctcgg gaaattacag caggcgcaag aaaagggcga catggaacaa
     1741 atcattaatg tgaacaggct atttcgctta gcgatttatc atcgctcaaa tatgctcatc
     1801 ctgtgtgaga tgattgagca actgtggatc aggatgggac ctgctttaca ttatctttat
     1861 gaagcgatta acccagcgga attacgggag cgcatagaaa actatcatct attactcgct
     1921 gcgttaaaag caaaagacaa agagggatgc aaacattgtc ttgctgaaat tatgcaacaa
     1981 aatattgcta ttttatacca gcaatacaat cgttaaatta aatgcccggc aaataccggg
     2041 catcaagtaa ttactcaaat cgccacgcaa tattcatgcc gacaccataa tttcgtccat
     2101 cgataggaat taaaacccca aaaagattaa aaaaacacca caaaacggat gtttcttcaa
     2161 caccactttt gctccatatg aacggaaccg acgattaaac tggatggctc tgattgattc
     2221 agggtatgaa tggcggtttt ttgctccgtt tccctcaaaa tggacgcaac ttcccctctg
     2281 cggctctcag ccgcaccacc gcatccgggc cagcggctca tgcatcagga cctgctctgc
     2341 cagacggtag ccccgcttca gccccgtaaa acgcatctga ctcccgcaca gcacgcactt
     2401 cagcgggtca accttcagta acctctgata catccctctc caggtgattt gcatcgccgt
     2461 ttttctcact gtctccgtta tgatgtacac cacttcttcc agtaaccgcc gtttcgccgg
     2521 actcaaaaaa ccgtagtacc tcaccatacg gaacccctta tccgccacat gccaggagaa
     2581 cctttccatg aactcatctc cactcatcaa caggtattct tcccgttttg ttcggtgact
     2641 gttgtaacgc agaccgattt catcctgacc ggcataatgc tccagacgac tcatcggcac
     2701 cggtggcttt ttcaggtaag agccaaagta caccgccaca tgggtggcat tatccattac
     2761 ccgggatacg ttgacattcc agccacggcg gtaatgcgtg tccaggaagc gattccattc
     2821 ccgtttactg cttccttctg ctgccagcgc atccggcatc accaggtcag ggtatttccg
     2881 tgacagcaac cgtgttatcc ggtagcgcca catgctcatc accttacggg cgtaaaaatg
     2941 aagatttttc caggtgtggc ccgacgtcac accaccggca gttgtcgata aatggatatg
     3001 cggatgccac tgctggtcac gcccccatgt gtggatcacc gtgaatatcc ccggctccac
     3061 atctgcctga tggcagattt ccagtatcac atccgctgca atgcggctca tctctgccag
     3121 taaccaccgg ttgtggaaca ccagggacca gtactggcag ggaagtgtga acacaatatg
     3181 ctgccacggg cagtagggga ccaggctcag cagatactgt atccactgtg cgccagcctt
     3241 caccccgcag tgcgggcagg agcggctttt acaccggaag cagacctttt ttgtatggca
     3301 acagtccggt gatgaacaac accactgcgt gtagcccatc agtgtggtcc cgcaggccat
     3361 gatttttgtc actgattcaa ttaccaccgg acggactgcc ccttccggtt gtttctccag
     3421 ccagttaagc cagcggtttc cctgctgaaa aatatcggca aaacggggga gcatcag
//
LOCUS       DAEEUE010000211         3285 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.211, whole genome shotgun sequence.
ACCESSION   DAEEUE010000211 DAEEUE010000000
VERSION     DAEEUE010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.211"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..317
                     /locus_tag="KLK84_004112"
     CDS             <1..317
                     /locus_tag="KLK84_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HBE2011212.1"
                     /translation="VEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVM
                     RMLMTLQGASPGRIPELMSDLASMGQLVKLPTRRGRAFPRVVKERPWKYPTAPKKSQS
                     VA"
     gene            475..2343
                     /gene="kup"
                     /locus_tag="KLK84_004113"
     CDS             475..2343
                     /gene="kup"
                     /locus_tag="KLK84_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HBE2011213.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2510..2929
                     /gene="rbsD"
                     /locus_tag="KLK84_004114"
     CDS             2510..2929
                     /gene="rbsD"
                     /locus_tag="KLK84_004114"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBE2011214.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2937..>3285
                     /locus_tag="KLK84_004115"
     CDS             2937..>3285
                     /locus_tag="KLK84_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBE2011215.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          753 a          767 c          878 g          887 t
ORIGIN      
        1 ttgtggagca agagctgtgg ggtgtcttac tggcttataa tctggtgaga tatcagatga
       61 ttaaaatggc ggaacatctg aaaggttact ggccgaatca actgagtttc tcagaatcat
      121 gcggaatggt gatgagaatg ctgatgacat tgcagggcgc ttcaccggga cgtataccgg
      181 agctgatgag cgatcttgca agtatgggac aacttgtgaa attaccgacg agaaggggaa
      241 gggccttccc gagagtggta aaggagaggc cctggaaata ccccacagcc ccgaaaaaga
      301 gccagtcagt tgcttaactg actggcatta ctcaaagggg gggctttttc tttgagccag
      361 gttaatgtgg ccgcatttag gagtacgatt ttgccgttaa tcgtgcatac tgtgcgcttt
      421 tttgtgggcc aagggactaa gcacacattt catatttcaa cgaaagacta gtctatgagc
      481 actgataata agcaatcatt gcccgcgatt accctcgcgg cgattggagt tgtctacggc
      541 gatattggta ccagcccgtt atatacactt cgtgaatgtt tgtccggcca gtttggtttt
      601 ggcgttgaac gcgatgccgt gtttggcttt ttatcgctga tcttctggct gctaatcttt
      661 gtggtttcca ttaaatatct caccttcgtg atgcgggcag ataacgccgg tgaggggggg
      721 atcctgacgt tgatgtcgct tgccgggcgt aatacgtcgg cgcgaaccac atcaatgctg
      781 gtgattatgg ggctaatcgg cggcagcttt ttctatggtg aagtcgtcat aacacccgct
      841 atttcggtga tgtcagccat tgaaggtctg gaaatcgtcg ccccgcagct ggatacctgg
      901 atagttcccc tctcaattat cgttctcact ttattattta tgattcaaaa gcatggcacg
      961 gctatggtcg gtaagctgtt tgcgccgatc atgctgacct ggtttttgat tctggcagga
     1021 ctggggttac gtagcattat tgctaacccg gaagtgctgc atgcgctgaa tccgatgtgg
     1081 gcggtgcatt tcttccttga atacaaaacg gtttctttta ttgcattagg ggcagtggtg
     1141 ctgtcgatta cggggggcga ggtgctgtat gctgatatgg ggcactttgg taagttccct
     1201 attcgcctgg cgtggttcac cgtcgtatta ccttccttga cccttaatta cttcggtcag
     1261 ggagcgttgt tgttaaagaa cccggaagcg attaagaacc cgttcttcct gttggcaccg
     1321 gactgggcgc tgatcccact gctgatcatc gccgcactgg cgacggtaat tgcctcgcag
     1381 gcggttatct ctggcgtctt ctcattgacg cgtcaggcgg tacgtctggg atatttgtcg
     1441 ccgatgcgca ttattcacac ctccgaaatg gagtcagggc aaatctatat tccgtttgtg
     1501 aactggatgc tctatgtcgc ggtcgtgatt gtgattgtca gctttgagca ctccagcaac
     1561 ctggcggcgg cgtacgggat tgcggtgacc ggaaccatgg tactgacgtc tattctctcg
     1621 actaccgtgg cacgtcagaa ctggcactgg aataagtatt ttgttgcgct gatcctgatt
     1681 gctttccttt gtgtcgatat tccattgttc accgctaacc tcgataaact gctctccggc
     1741 ggctggttgc cattgagcct cggtactgtg atgtttatcg tgatgaccac ctggaagagc
     1801 gagcgtttcc gcttgctgcg gcggatgcat gaacatggta actctctgga agcgatgatt
     1861 gcttcgctgg agaaatcacc gcctgttcgc gtgcccggga ccgcggtgta tatgtcgcgt
     1921 gcaatcaacg tcattccctt tgcgctgatg cataacctta aacataacaa ggtattgcat
     1981 gagcgggtga ttctgttaac tctgcgcacc gaagacgcgc catatgtcca taacgtccgt
     2041 cgggtacaga ttgaacaact gtcgcccact ttctggcgcg tggtggcaag ttatggttgg
     2101 cgagaaacgc caaacgtgga agaagttttc caccgctgcg gtctggaagg attaagttgc
     2161 cggatgatgg aaacctcctt ctttatgtcg catgagtcgt tgatcctcgg caaacgcccg
     2221 tggtatttgc gtctgcgcgg caagctgtac ttgctgctgc aacgtaatgc gctgcgtgca
     2281 ccagatcaat ttgaaatccc gccaaacagg gttatcgaac tgggtactca ggtcgaaatc
     2341 taacgccaga cgcctccttt cttcataagg gggcgttttt gttttcatgg ttaatcacca
     2401 tgtaaaacgt ttcgaggttg atcacatttc cgtaacgtca cgatggtttt cccaactcag
     2461 tcaggattaa gctgcggatc agcgaaacgt ttcgctgatg gagaaaaaaa tgaaaaaagg
     2521 caccgttctt aattctgata tttcatcggt gatctcccgt ctgggacata ccgatacgct
     2581 ggtggtgtgt gatgctggtt tacccatccc caaaagtaca acgcgtatcg atatggcatt
     2641 aacccaaggt gtaccttctt ttatgcaggt gctgggcgtc gtcacaaatg aaatgcaggt
     2701 cgaggctgcc attatcgcgg aagagatcaa acaacataat ccgcaactcc acgaaacgtt
     2761 gctcactcac cttgagcagc tgcaaaaaca ccagggaaat accattgaaa ttcgttacac
     2821 cacgcatgaa caattcaaac aacaaaccgc agaaagtcag gcggtaattc gcagcggaga
     2881 atgttctccg tatgcgaata tcattctctg tgctggcgtg acgttctgag gccgtcatgg
     2941 aagcattact tcagcttaaa ggcatcgata aagccttccc gggcgtaaaa gccctctcgg
     3001 gcgcagcgtt aaatgtctat ccgggccgtg tgatggcgct ggtgggcgaa aacggcgcgg
     3061 gtaaatccac catgatgaaa gtgcttactg gtatctatac tcgcgatgcc ggcacgcttt
     3121 tatggctggg gaaagaaacg acatttaccg ggccgaaatc ttcccaggaa gccgggattg
     3181 ggattatcca tcaggaactg aacctgatcc cgcagttgac cattgccgaa aacattttcc
     3241 tcggtcgtga gtttgttaat cgctttggca aaattgactg gaaaa
//
LOCUS       DAEEUE010000212         3230 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.212, whole genome shotgun sequence.
ACCESSION   DAEEUE010000212 DAEEUE010000000
VERSION     DAEEUE010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.212"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(438..1022)
                     /locus_tag="KLK84_004116"
     CDS             complement(438..1022)
                     /locus_tag="KLK84_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBE2011216.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(1022..2260)
                     /locus_tag="KLK84_004117"
     CDS             complement(1022..2260)
                     /locus_tag="KLK84_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE2011217.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            complement(2257..3162)
                     /locus_tag="KLK84_004118"
     CDS             complement(2257..3162)
                     /locus_tag="KLK84_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HBE2011218.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
BASE COUNT          661 a         1037 c         1001 g          531 t
ORIGIN      
        1 gccgtgctag tctgctcggt gatggtggag tgaagccaac ccgcaatcgg gttatgaatc
       61 tgcatcgcga ttcgcaatca gctgtctctt gagcatgtcg aactcctgcg atgttatctc
      121 gccgtgctcg tgctgctaca ccaacttgtc caactcatcc gccacaccgc tccccaccac
      181 ctgcgatggc caggttgttt gctgggcgca ttggcggagt atcgtctcga cccgggacac
      241 ccacaccggc acaaccttac gggagtgatt cactgtcaaa gaatcggccc ggtgctctga
      301 cgcaagtatc gggatggtca ccatttgtaa gccgtagacc tgagtggtga tcaagacttc
      361 gataccaccg accgtaccgg tactaatcga cgacggtcgt gttcgtcgcc tgccgcaggg
      421 actctgcaca cctccgttta cgcatgtgcc tggaggagtt ggaaatcgtc gtgttcggga
      481 aacattaaac acaggatggc agcgatctga gccagcacat gatcagctag ctcaccatcc
      541 ggatcgacgg cccactgcat cgtcgcgcca gcgatgaccg agtgcaggag caactcagct
      601 gccgcaggag cacctggggg cagtcgcttg cggatcccct ccaccaccgc gcggttccgc
      661 tggatcgcaa gcgtgcgtag ctccggcacc tggagctcgt accaggagat gagatagttc
      721 accgagaagt cgttgcgagt gttcatgctc cgaacgagca cctgcaaaaa ttcccagagc
      781 ccttgcggcc ctgcgcctat cggtatcgca ttcaggtaat gccgcacctg ctcgacgccg
      841 cgctccatca tcctcaccag cagcgtatcg cggttggtga agcgctggat taacgctgcg
      901 cgggagagcc ccacctcctt tgctactccg ctgagcgtga actctatggg accgcaacgc
      961 ttcagcacta cggtggcggc ctcgagtacc tcgtcatcgg acttgagctt ggggcggggc
     1021 atcagtgttc accttctgta tgggttgggg cggaggctgt ggctgccgcc gccattgtag
     1081 caaattgaag acggagcgag agtagagcca cgagccccgc aaacacggcc gatacaacga
     1141 ggccagggag cggaccagca aggtcgacaa acgcgccggc cgcaagcata accatgggcg
     1201 aggctgacag catcaccgcc gagaccgtgc cgagtacccg gccgagaagt tctggcggcg
     1261 tgcggttgta gatggcagcg ttgagaatgg gagagactga gccggtcagc agtcccacga
     1321 gcgcgcccaa caacatcagc accggcacgc ctggcaactg tgaaagcaga agcgagccca
     1381 ccgcagagcc acaaaatgcc accgccagcc agttctgcgc tgatatccgg gcgccgaccg
     1441 acgcatgaat ggcaatgcca aggagaccac cagcccccat cattgaggag aacagcccga
     1501 gctctgctac ttggcgtcct gcatctacaa acagcgcagg catgatgacg ctgccgttgg
     1561 cgccaacgat gcccacgaag atcatcacta taccaaagag agggcgcagc aggggttcgc
     1621 tccagagaaa agcgacgccg gcgcgcatgg agagagtcgc cgtcgtggtc atcgtccgag
     1681 cggcacgcgc gggaagcacc cacgcgccga gcagacctgc aaggacggag cagaacgccg
     1741 tcagcccgag cgttggcgca gcgccaagca ggccgattgc ggccccccca agggccgggc
     1801 cacctagaat cgcgacgttc ccgatcaccg ctttcagtga cgagacgcgc tcaacggaga
     1861 gcccggcgac gtggccgagt ttgggcagct cactgtcctg cgcggccata ccgggtgcgt
     1921 cgaacgcggc accgagcacc acgcaagcga tcagcccagt gttcgagagg gcgccaacgg
     1981 catcgagcag tgggatgctc gccatggcca cgccgcccac cacacccgag atcaatgcga
     2041 cgggcgcgcg cccgaaccga tcgacgaggc caccaccaac ccacgcgccg atgatggtcg
     2101 cgatgacgct gctagcggcc gtggcgcccg cccaggccgc gctctttgta tgagacagga
     2161 cgaaccatgg aagcgcgagg gccgccaccg cgttgccgat ccggaagaga aaggtcgccg
     2221 cgaacagcgt cgcgagcggg ctatatcgac gttcgctcat tccgctgcgg cgagctgcgc
     2281 cttcgccgca gcgaggtact cttcgttacc cgagtcgagg gcgaagagtg cgtaggtgac
     2341 cgccccgaac gcaaggcgct ccgcgatgtg gtgggcgagc cgcggccaca cccggccacc
     2401 ggccgcttca tacgtgagga ggagcttcgc gagcccctct tcaccaaaga ccataaggtg
     2461 cgcggccatg tcgatggcag ggtcatcaac gcgggcctcg ctccagtcga tcatcccgct
     2521 gacgcgctcc gtgttgtcga tgagcacatg gcccacgtag agatcgccat gcaccaccac
     2581 ggagaaatct ggccacgacg aatcgtcgtc gagccagcgc tgccaccggt ggaggcgctt
     2641 gtcgttcacc acgaactcgc gtcggacgcg gtcaacgtcg tcggccacct tctgacgggc
     2701 ctgcgtcggt gtacggatga gcatccccgc atccacggcg gcggaaatgg ggacggcatg
     2761 cagggcggcg agcgcggtcg cgaagctctc cgcgaagacc tccgagtcct gcggcacgac
     2821 ccagtcgggc gtggacgaac caggctggat gaccatcgca gtcgagtctt cgagcatggg
     2881 ataggcaacg agctcggcgt tggccacgcg ccagtccggc accgcgaacg gcaggcgatt
     2941 cttgagcatt gccagcaccc gcgcctctgg ttcgaccttc gcgcttacct cggctcggcg
     3001 cgggatgcgc agcacccacc gacgtccatc gtcgacggtg gcgatcacga tcctatagtc
     3061 gagcccaagc tcattgacag tcagcgggcc atggagcttg agcccatgtc gggctgcaag
     3121 tgcgtacagt tgggaggtat cggcggtcgt gactacggtc atgattcact cctgagggct
     3181 tgacgggttt agccacctaa atgtaacagt cacgtcggtt atattcaatc
//
LOCUS       DAEEUE010000213         3191 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.213, whole genome shotgun sequence.
ACCESSION   DAEEUE010000213 DAEEUE010000000
VERSION     DAEEUE010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.213"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            174..1277
                     /gene="proB"
                     /locus_tag="KLK84_004119"
     CDS             174..1277
                     /gene="proB"
                     /locus_tag="KLK84_004119"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBE2011219.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1289..2542
                     /gene="proA"
                     /locus_tag="KLK84_004120"
     CDS             1289..2542
                     /gene="proA"
                     /locus_tag="KLK84_004120"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBE2011220.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2657..2732
                     /locus_tag="KLK84_004121"
     tRNA            2657..2732
                     /locus_tag="KLK84_004121"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2690..2692,aa:Thr,seq:cgt)
     gene            complement(2747..>3191)
                     /locus_tag="KLK84_004122"
     CDS             complement(2747..>3191)
                     /locus_tag="KLK84_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE2011221.1"
                     /translation="SKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTK
                     APTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWES
                     IEMVRRYAHLAPNHLTEHARQIDSILNPSVPNLSQSKNKEGTNDV"
BASE COUNT          756 a          807 c          879 g          749 t
ORIGIN      
        1 aaaatgtggt aatttattaa atctgtaata aaagcgtaaa caactgccgc tacgcttgct
       61 gatcccgcgc aacaaaacgc catgctttgc tcgcagatgg ttggcaaccg acgacagtcc
      121 tgctaaaacg ttcgtttgat atcatttttc ctaaaattga atggcagaga atcatgagtg
      181 acagccagac gctggtggta aaactcggca ccagtgtgct aacaggcgga tcgcgccgtc
      241 tgaaccgtgc ccatatcgtt gaacttgttc gccagtgcgc gcagttacat gccgccgggc
      301 atcggattgt tattgtgacg tcgggcgcga tcgccgccgg acgtgagcac ctgggttacc
      361 cggaactgcc agcgactatc gcctcgaaac aactgctggc ggcggtaggg cagagtcgac
      421 tgattcaact gtgggaacag ctgttttcga tttatggcat tcacgtcggg caaatgctgc
      481 tgacccgtgc tgatatggaa gaccgtgaac gcttcctgaa cgcccgcgac accctgcgtg
      541 cgttgctcga taacaatatc gttccggtaa tcaatgagaa cgatgctgtc gctacggcag
      601 agattaaggt cggcgataac gataaccttt ctgcgctggc ggcgattctg gcgggtgccg
      661 ataaactgtt gctgctgacc gatcaaaaag gtttgtatac cgctgatccg cgcagaaatc
      721 cgcaggcaga actgattaaa gatgtttacg gcattgatga cgcactgcgc gcgattgccg
      781 gtgacagcgt ttcaggcctc ggaactggcg gcatgagtac taaattgcag gccgctgacg
      841 tggcttgccg tgcgggtatc gacaccatta ttgccgcggg cagcaagccg ggcgttattg
      901 gtgatgtgat ggaaggcatt tccgtcggta cgctgttcca tgcccaggcg actccgcttg
      961 aaaaccgtaa acgctggatt ttcggtgcgc cgccggcggg tgaaatcacg gtagatgaag
     1021 gggcaactgc cgccattctg gaacgcggca gctccctgtt gccgaaaggc attaaaagcg
     1081 tgactggcaa tttctcgcgt ggtgaagtca tccgcatttg caacctcgaa ggtcgcgata
     1141 tcgcccacgg tgtcagtcgt tacaacagcg atgcattacg ccgtattgcc ggacaccact
     1201 cgcaagaaat tgatgcaata ctgggatatg aatacggccc ggttgccgtt caccgtgatg
     1261 acatgattac ccgttaagga gctggccgat gctggaacaa atgggcattg ccgcgaagca
     1321 agcctcgtat aaattagcgc aactctccag ccgcgaaaaa aatcgcgtgc tggaaaaaat
     1381 cgccgatgaa ctggaagcac aaagcgaaat catcctcaac gctaacgccc aggatgttgc
     1441 tgacgcgcga gccaatggcc ttagcgaagc gatgcttgac cgtctggcac tgacgcccgc
     1501 acggctgaaa ggcattgccg acgatgtacg tcaggtgtgc aacctcgccg atccggtggg
     1561 gcaggtaatc gatggcggcg tactggacag cggcctgcgt cttgagcgtc gtcgcgtacc
     1621 gctgggggtt attggcgtga tttatgaagc gcgcccgaac gtgacggttg atgtcgcttc
     1681 gctgtgcctg aaaaccggta atgcggtgat cctgcgtggt ggtaaagaaa cctgtcgcac
     1741 taacgcggca acggtggcgg tgattcagga cgccctgaaa tcctgtggct taccggcggg
     1801 tgccgtgcag gcgattgata atcctgaccg tgcgctggtc agtgaaatgc tgcgtatgga
     1861 taaatacatc gacatgctga tcccgcgtgg tggcgctggt ttgcataaac tgtgccgtga
     1921 acagtcgaca atcccggtga tcacaggtgg tataggcgta tgccatattt acgttgatga
     1981 aagtgcagag atcgctgaag cattaaaagt gatcgtcaac gcgaaaactc agcgtccgag
     2041 cacatgtaat acggttgaaa cgttgctggt gaataaaaac atcgccgata gcttcctgcc
     2101 cgcattaagc aaacaaatgg cggaaagcgg cgtgacatta cacgcagatg cagctgcgct
     2161 gacacagttg caggcaggcc ctgcgaaggt ggtggcggtt aaagccgaag agtatgacga
     2221 tgagtttctg tcattagatt tgaacgtcaa aatcgtcagc gatcttgacg atgccatcgc
     2281 ccatattcgt gaacacggca cacaacactc cgatgcgatc ctgacccgcg atatgcgcaa
     2341 cgcccagcgt tttgttaacg aagtggattc gtccgctgtt tacgttaacg cctctacgcg
     2401 ttttaccgac ggcggccagt ttggtctggg tgcggaagtg gcggtaagca cacaaaaact
     2461 ccacgcgcgt ggcccaatgg ggctggaagc actgaccact tacaagtgga tcggcattgg
     2521 tgattacacc attcgtgcgt aaataaaacc gggtgatgca aaagtagcca tttgattcac
     2581 aaggccattg acgcatcgcc cggttagttt taaccttgtc caccgtgatt cacgttcgtg
     2641 aacatgtcct ttcagggccg atatagctca gttggtagag cagcgcattc gtaatgcgaa
     2701 ggtcgtaggt tcgactccta ttatcggcac cattaaaatc aataagttac acatcattag
     2761 taccttcctt attttttgac tgggacaaat ttgggaccga tgggttcagg atcgagtcta
     2821 tttgccgtgc gtgttcggta aggtgattag gtgcaaggtg agcatatcga cgaaccattt
     2881 cgatagactc ccagcctccc atttcctgta acactgacaa cgggactccg gcttgaacca
     2941 gccaacttgc ccaggtgtgt ctcaagtcgt gaaatctgaa atcatcaata ccagcccgtc
     3001 tcagcgccgc tttccaggct gtgtttgcgt cataccgcat cttccttact gttggcgctt
     3061 tcgttccgtc tggtttggta cagctttcct tgtacacaaa tacccaacgg tgatgattcc
     3121 cgatttgttt tttcaatacg cgacatgcag tatcattcag cgcaacgcca attgcgcggt
     3181 ttgatttact c
//
LOCUS       DAEEUE010000214         3179 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.214, whole genome shotgun sequence.
ACCESSION   DAEEUE010000214 DAEEUE010000000
VERSION     DAEEUE010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.214"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            417..1526
                     /gene="fepE"
                     /locus_tag="KLK84_004123"
     CDS             417..1526
                     /gene="fepE"
                     /locus_tag="KLK84_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HBE2011222.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVGFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2551..2745)
                     /locus_tag="KLK84_004124"
                     /pseudo
     CDS             complement(2551..2745)
                     /locus_tag="KLK84_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          800 a          658 c          776 g          945 t
ORIGIN      
        1 aaaaaaaaca tcatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgccctggg ggcgctgcta ggggtgtctg ttcagatatg tagcaatgag tcagggattc
      121 acgaactgaa aatgtccgaa aatgtgtctc atgggcactg tatgcgccca aaccgatcgt
      181 cattcacttt catgcatagc tatgcagtga gctgaaagcg atcctgacgc atttttccgg
      241 tttaccccgg ggaaaacatc tctttttgcg gtgtttgcgg cagaatcgcg ttcagcgcgt
      301 tttagcggtg cgcgcaataa aatgttatgt taaatgtctg ctgaatggtt tttaggtttt
      361 ttcctaacat atgatagaat aaaaaaacca tatgttaata tattgtgttt ttttcaatga
      421 aaatctcaaa ggtctctgac gaacattttt ctaattacaa catgttgccg aatggtaatg
      481 aagaaattga cctttttggc ttgattagcg ttttatgggc agcaaaaata aaaattgttg
      541 gggtgattct attttttgct tgtattggct ttcttgtttc ttttgttttg ccacaaaaat
      601 ggactagttc tgctgtggtt actcctgctg agtctgttca atggagtgaa ttaaataagg
      661 agctttccaa gcttcatgtt ctgggagtgg gttttgatat caatcgtgat agcgcatttg
      721 ctctgtttat taagaaattt cagtccgtta attcgttgaa tgagtatatg agatcatctc
      781 cttatgttat ggagattatt aaaaagaata acattagcgc tctcgaactg catcgtgcaa
      841 ttgttgagtt gagtaaaaat atgaagtcga ttgatgataa tgccagtaag aaaaacgaca
      901 agtcatcatt gtatgtatca tggactctga gttttactgc tccaacaagt aaagaagctc
      961 acgatgtgtt gtctgggtat attaattatg tttcttccct tgttgtaagg gatttgatgg
     1021 aagatataag aaataaacta gaagttaaaa ctaatgttga aaaagaaatt cttgcactgg
     1081 atgagataaa aattagaaac cagctgaatg cagatattcg acgcctcaat tattcactgg
     1141 aggttgctaa tgcggctgga ataaaaaaac ctgtatacag caatggtcag attatgaagg
     1201 atgacccaga ttttcctgtg gctctcggtt ctgatggtat agcaactaaa ttgaacatca
     1261 aaaaatcaat caaggatgtt tcggaattga gtggggagtt gcgaaatcgt caatatgttg
     1321 tgaatcaatt ggttgtggcg aaagtggggg atgttgattt tatgccattt cagtatcaat
     1381 tgagcccaac gttaccggtc agaaaggatg ggccaagtaa tgccataatt gtaattcttt
     1441 cttctgtcat tggagggatg cttgcatgtg gctatatact tttacgtcat gccatggagg
     1501 ccagaaagat gagtgtgatt gagtaaaagt tgtgctgatg catcatttta taagcaactt
     1561 ttataaaaag ttcaaaggat cttcttgaga tcattccctt acagccgtag tcttctactc
     1621 tagaaaacga aaaaaccacc tggggaggtg gtttttcgaa ggttcagtaa gttggggaac
     1681 ttctgaaccg tggtaacagt cttgtgcgag acatgtcacc aaatctgtcc ttctagtgta
     1741 gcctcagtaa ggctgccact tcaagaactc tcaatacatc tctcgcacat cctgtttacc
     1801 agtggccgct gccaatggcg ttaagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1861 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1921 gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agacgaacgc ggccataaca
     1981 gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccaccag
     2041 ggggaaacgc ctggtatctt taagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     2101 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccaccg gcgcggcctt
     2161 ttttcggggc ctctcttttg ctcacatgtt ttttctggtg ttatcccctg attctgtgga
     2221 taaccgtata tccgtctttg cgtgagcaga caccgctcgc cgcagccgaa cgaccgagcg
     2281 cagcgagtca gtgagcgagg aagcggaaga gcgccggaag ctgcattttc tccttacgta
     2341 tctgtgcagc atttcacacc tcatatgtcg ctgttctctc accatctgct ctgccgcctc
     2401 ataattaagc cagtatacac tccgttatcg ctacgtgact gggtcatggc tgcgccccga
     2461 cacccgctaa aaccccctga cgcgccctga cgggcttgtc tgctgacgaa aacgcccacg
     2521 ggaaagccgt gaccgcctcc gtcagcttcg ttatgttcct gtttcacttt cggcctcaaa
     2581 gcgaatctgg atgctgttct ggagttcttc tgcgagttcg tgcagtcgtt tgcacatggc
     2641 cgcctgttcg tcggcatcca gagcatccag tttttcaagc agtgtcaggc tctggctttt
     2701 gatacatcgc gccatattca gcgcagtttg ttgttatttg ttcaccttcc gttgttctcc
     2761 gtcagttccg ggtgtcgggg ggggggaccc tgagcaggtg gtaattgtaa gacgttgcgg
     2821 gcgatggtat tacaattgca catcctgtcc tgtttcttta ggacgtatca tggcacagca
     2881 tatcaactga taaataattc ctgtgcagtg cagccgctgt tgcataaaat ttcgctctgc
     2941 gtcgagtgtt tcttaatcaa taattccgct gatactccgg aaaaacgcga gtctgttctc
     3001 atgcctgaaa tgcccacacc ccacgcaaaa acaagttttt gctgattttt ctttataaat
     3061 agagagttat gacaaattag ttcttcttgc tctctttgtg atatttaaaa aagcggtgtc
     3121 ggcgcggtgt tgtagctgcg ccaacaccgc tttttagggg tggtactgac tattttcat
//
LOCUS       DAEEUE010000215         3179 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.215, whole genome shotgun sequence.
ACCESSION   DAEEUE010000215 DAEEUE010000000
VERSION     DAEEUE010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.215"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(17..643)
                     /gene="dsbA"
                     /locus_tag="KLK84_004125"
     CDS             complement(17..643)
                     /gene="dsbA"
                     /locus_tag="KLK84_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HBE2011223.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(660..1646)
                     /gene="srkA"
                     /locus_tag="KLK84_004126"
     CDS             complement(660..1646)
                     /gene="srkA"
                     /locus_tag="KLK84_004126"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HBE2011224.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1723..1992)
                     /locus_tag="KLK84_004127"
     CDS             complement(1723..1992)
                     /locus_tag="KLK84_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HBE2011225.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2062..2646
                     /gene="mobA"
                     /locus_tag="KLK84_004128"
     CDS             2062..2646
                     /gene="mobA"
                     /locus_tag="KLK84_004128"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HBE2011226.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2628..3155
                     /gene="mobB"
                     /locus_tag="KLK84_004129"
     CDS             2628..3155
                     /gene="mobB"
                     /locus_tag="KLK84_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBE2011227.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT          834 a          773 c          764 g          808 t
ORIGIN      
        1 tgtaatttac attgaattat ttttcctcgg acagatattt caccgtatca gcatactgct
       61 gaacaaaaac atccatattg ctggtatcca taccctgcgg attcagctga tatttaccgt
      121 taacaaacat cgccggaaca ccacgcaatt gcacatcagc tgcggctttt tcctgctgag
      181 cgaccagaga tttcaccacg aagctgttcc acgccgcgtc gtactcttca cctttaatac
      241 ctgcattgat aaatacatcg cggatatcag atgctgaacg aatggtctgg gttttctgta
      301 cgccttcaaa cagcggaaca gtcactttgt cttccacgcc cagcgccatc gccacagccc
      361 atgcctgagt cagatctttg cccaggtcac cacccatgaa gttgacgtgg tatttagtca
      421 tcttcacgcc ttccggcagt tttttcttca cattatcaga aatatgcaga acttcttcaa
      481 actgatagca gtgcgggcag aagaaagaga aaaactccag cacttgcggc gcgccagcta
      541 ccggtttttc cagggtagtg tactgtttac catcttcata ctgcgccgcc gatgcgctaa
      601 acgctaaaac taaaccagcc agcgccagcc aaatcttttt catgatctac tctctccgat
      661 taatacatag gtgttaattg caaagggggt tcttgtagaa cttttgcctg ttctataaaa
      721 gtcgccgtct gtcgtagcca gtaatcttcc ccggttaacc acgggaaatt tttcgggaac
      781 gcgggatcag cccaatgccg cattagccag gcaagataat aaaccaaacg catggcgcgt
      841 aaaggttcaa tcagtccgat ttcagcggtg tcgaactcgc taaattcttc ataagcttca
      901 ataatagttt ccagttgcat ccgctgctgg gctttatcgc cattgagcaa catccacaaa
      961 tcctgaatag ctggaccatt acgtgcatca tccagatcaa caaacattgg accatcgcgc
     1021 cagagaatat tcccggcgtg gcagtctcca tgtagccgca gaacggtgaa atcttcccgc
     1081 cagtgtgctg taacggcggc gatcagctca tctgtcgctt tcagaaatgc cgctttcaac
     1141 ccggaaggta tcagtgtagc gtcctcaaac agcttgcgag gctcaatgag atattcattc
     1201 agaccgatgg tcgggcgatg aataaaaagc tgtttgcgcc ccgtctggtg catacgccct
     1261 aaataacgcc caaccgcttc catctgatcg atattatcag cttcgaactg gcgaccaccg
     1321 acgcttggaa aaacagcgaa ataaaatccc tgatgattca ataaagtctg accgttaaag
     1381 gccacaggtg ctgcgaccgg aacttcatca tttaccagct gcaacgcaaa ttgatgttct
     1441 tcgaggattt gatcggctgt ccaacgttca gggcgataaa atttgacgac aaaacgtcga
     1501 cgctcttcgt cctgaaattg atagacacgg ttttcatagc tgttaagcgg ggtaagaccg
     1561 gaatccaccc ggatcccttg ctcaaacaga gcgtccatga tggtatccgg gtgtagtgtc
     1621 tggaaagtaa aagcgctgtt gttcatccca tcatccggaa aacattacga atgatgaagg
     1681 atatcatttc acgatgattt tggtggcgcc gcttacaagc ttttactctt taattacgcc
     1741 gcgagcgcgt aacagcgccg ttttgaagtc ttcttcataa tctttctgca aacccgggat
     1801 caccgcatct ttcgcggaat cacgcatttt caggtgatag atcagaatgt catccgtcaa
     1861 atccgccagt tcgccgtcaa aacctgactc tttcgccagt ttctgcaaaa attgcaacaa
     1921 gttaaagtct ggctcttttt gccaggctgg ctggaggagt tcaataactt cattcagacg
     1981 tttacatttc atagtaatgc tccttactct tgagacagac acgttagcag ggtcaatccc
     2041 acaataaaag aggcgatatc ggtgaatctg atgacgacga taacaggcgt tgtgctggca
     2101 ggcggtaaag ccagacgaat gggcggcgta gataaaggat tgctggactt aaatggcaaa
     2161 ccgctgtggc aacatgtcgc tgacgcgctt aagacgcagc tctctcacgt cgtggttaat
     2221 gctaatcgtc atcaggaaat ctatcaggca ggcggtctga aagtgattga agattcactg
     2281 gcggattacc caggccctct ggcaggaatg ctttcagtaa tgcagcagga agctggtgag
     2341 tggtttttgt tttgtccgtg cgatacgcct tacattcccc atgatttagc cgcccggctt
     2401 aatcatcagc gcaaagatgc gcctgtcgtg tgggtccatg acggtgaacg cgatcacccg
     2461 actattgctc tggtaaaccg cgctattgag cctttattac tggaatatct gcaagcagga
     2521 gaacgccggg taatggcatt tatgcgtctg gctggcggtc atgcggttga tttcagcgat
     2581 cataaagatg catttgttaa cgtgaatacg ccagaggagc ttgcccgatg gcaggaaaaa
     2641 cgatgatacc gttactcgcc tttgccgcgt ggagtggtac cggaaaaact acgcttttga
     2701 aaaaactgat cccggcatta tgcgccagag ggatccgtcc ggggctgatt aagcatacgc
     2761 accatgatat ggatgttgat aagccaggca aagatagcta tgagctgcgc aaggctggcg
     2821 cggcacaaac catcgttgcc agccagcaac gatgggcctt gatgacggaa acaccagacg
     2881 aagaagagct ggatctacat tttctcgcaa gtcgaatgga tacctcaaag ctggatttga
     2941 ttctggtcga agggtttaag catgaagaga tcgcaaagat tgtgctgttt cgcgatggag
     3001 ccggacatcg acctgaagaa ttagtgatag acaggcatgt tattgctgta gccagtgatg
     3061 tgccgcttaa tcttgatgtc gcattactgg atattaatga tgttgagggg ctggctgatt
     3121 ttgtggtgga gtggatgcaa aagcagaacg gataagcaag agtaggctgg atcagacgc
//
LOCUS       DAEEUE010000216         3023 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.216, whole genome shotgun sequence.
ACCESSION   DAEEUE010000216 DAEEUE010000000
VERSION     DAEEUE010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.216"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            8..2911
                     /locus_tag="KLK84_004130"
     rRNA            8..2911
                     /locus_tag="KLK84_004130"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          800 a          658 c          946 g          619 t
ORIGIN      
        1 gttgtgaggt taagcgacta agcgtacacg gtggatgccc tggcagtcag aggcgatgaa
       61 ggacgtgcta atctgcgata agcgtcggta aggtgatatg aaccgttata accggcgatt
      121 tccgaatggg gaaacccagt gtgattcgtc acactatcat taactgaatc cataggttaa
      181 tgaggcgaac cgggggaact gaaacatcta agtaccccga ggaaaagaaa tcaaccgaga
      241 ttcccccagt agcggcgagc gaacggggag cagcccagag cctgaatcag tgtgtgtgtt
      301 agtggaagcg tctggaaagg cgtgcgatac agggtgacag ccccgtacac aaaaatgcac
      361 atactgtgag ctcgatgagt agggcgggac acgtggtatc ctgtctgaat atggggggac
      421 catcctccaa ggctaaatac tcctgactga ccgatagtga accagtaccg tgagggaaag
      481 gcgaaaagaa ccccggcgag gggagtgaaa aagaacctga aaccgtgtac gtacaagcag
      541 tgggagcacg cttaggcgtg tgactgcgta ccttttgtat aatgggtcag cgacttatat
      601 tctgtagcaa ggttaaccga ataggggagc cgaagggaaa ccgagtctta actgggcgtt
      661 aagttgcagg gtatagaccc gaaacccggt gatctagcca tgggcaggtt gaaggttggg
      721 taacactaac tggaggaccg aaccgactaa tgttgaaaaa ttagcggatg acttgtggct
      781 gggggtgaaa ggccaatcaa accgggagat agctggttct ccccgaaagc tatttaggta
      841 gcgcctcgtg aattcatctc cgggggtaga gcactgtttc ggcaaggggg tcatcccgac
      901 ttaccaaccc gatgcaaact gcgaataccg gagaatgtta ttacgggaga cacacggcgg
      961 gtgctaacgt ccgtcgtgaa gagggaaaca acccagaccg ccagctaagg tcccaaagtc
     1021 atggttaagt gggaaacgat gtgggaaggc ccagacagcc aggatgttgg cttagaagca
     1081 gccatcattt aaagaaagcg taatagctca ctggtcgagt cggcctgcgc ggaagatgta
     1141 acggggctaa accatgcacc gaagctgcgg cagcgacgct tatgcgttgt tgggtagggg
     1201 agcgttctgt aagcctgtga aggtgtgctg tgaggtatgc tggaggtatc agaagtgcga
     1261 atgctgacat aagtaacgat aaagcgggtg aaaagcccgc tcgccggaag accaagggtt
     1321 cctgtccaac gttaatcggg gcagggtgag tcgaccccta aggcgaggcc gaaaggcgta
     1381 gtcgatggga aacaggttaa tattcctgta cttggtgtta ctgcgaaggg gggacggaga
     1441 aggctatgtt ggccgggcga cggttgtccc ggtttaagcg tgtaggctgg ttttccaggc
     1501 aaatccggaa aatcaaggcc gaggcgtgat gacgaggcac tacggtgctg aagcaacaaa
     1561 tgccctgctt ccaggaaaag cctctaagca tcaggtaaca tcaaatcgta ccccaaaccg
     1621 acacaggtgg tcaggtagag aataccaagg cgcttgagag aactcgggtg aaggaactag
     1681 gcaaaatggt gccgtaactt cgggagaagg cacgctgata cgtaggtgaa gcgacttgct
     1741 cgtggagctg aaatcagtcg aagataccag ctggctgcaa ctgtttatta aaaacacagc
     1801 actgtgcaaa cacgaaagtg gacgtatacg gtgtgacgcc tgcccggtgc cggaaggtta
     1861 attgatgggg ttagcgcaag cgaagctctt gatcgaagcc ccggtaaacg gcggccgtaa
     1921 ctataacggt cctaaggtag cgaaattcct tgtcgggtaa gttccgacct gcacgaatgg
     1981 cgtaatgatg gccaggctgt ctccacccga gactcagtga aattgaactc gctgtgaaga
     2041 tgcagtgtac ccgcggcaag acggaaagac cccgtgaacc tttactatag cttgacactg
     2101 aacattgagc cttgatgtgt aggataggtg ggaggctttg aagtgtggac gccagtctgc
     2161 atggagccga ccttgaaata ccacccttta atgtttgatg ttctaacgtg gacccgtgat
     2221 ccgggttgcg gacagtgtct ggtgggtagt ttgactgggg cggtctcctc ctaaagagta
     2281 acggaggagc acgaaggttg gctaatcctg gtcggacatc aggaggttag tgcaatggca
     2341 taagccagct tgactgcgag cgtgacggcg cgagcaggtg cgaaagcagg tcatagtgat
     2401 ccggtggttc tgaatggaag ggccatcgct caacggataa aaggtactcc ggggataaca
     2461 ggctgatacc gcccaagagt tcatatcgac ggcggtgttt ggcacctcga tgtcggctca
     2521 tcacatcctg gggctgaagt aggtcccaag ggtatggctg ttcgccattt aaagtggtac
     2581 gcgagctggg tttagaacgt cgtgagacag ttcggtccct atctgccgtg ggcgctggag
     2641 aactgagggg ggctgctcct agtacgagag gaccggagtg gacgcatcac tggtgttcgg
     2701 gttgtcatgc caatggcact gcccggtagc taaatgcgga agagataagt gctgaaagca
     2761 tctaagcacg aaacttgccc cgagatgagt tctccctgac cctttaaggg tcctgaagga
     2821 acgttgaaga cgacgacgtt gataggccgg gtgtgtaagc gcagcgatgc gttgagctaa
     2881 ccggtactaa tgaaccgtga ggcttaacct tacaacgccg aagatgtttt ggcggattga
     2941 gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag
     3001 aattagcctg gcggccgtag cgc
//
LOCUS       DAEEUE010000217         2988 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.217, whole genome shotgun sequence.
ACCESSION   DAEEUE010000217 DAEEUE010000000
VERSION     DAEEUE010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.217"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            39..1436
                     /gene="ccp"
                     /locus_tag="KLK84_004131"
     CDS             39..1436
                     /gene="ccp"
                     /locus_tag="KLK84_004131"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HBE2011228.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1647..>2988
                     /locus_tag="KLK84_004132"
     CDS             1647..>2988
                     /locus_tag="KLK84_004132"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBE2011229.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKQGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKY"
BASE COUNT          756 a          689 c          792 g          751 t
ORIGIN      
        1 tctgggtcac aataactcac caccgtgagg gaagaataat gaaaatggtc tcacgtatta
       61 ccgcgatcgg cctggctggc gtcgcgattt gctatttagg gttatctggt tatgtgtggt
      121 accacgataa taaacgcagt aaacaggccg atgttcaggc atctgctgtc agtgaaaata
      181 ataaggtttt aggctttctc cgcgaaaaag gatgcgacta ttgccacacg ccttcggcag
      241 aattacccgc ctattattat attcctggcg cgaaacagtt gatggattac gacattaagc
      301 ttggatataa atcttttaac ctcgaggccg tgcgtgcggc actgctggct gataaacccg
      361 tttcgcaaag cgatctgaat aagattgaat gggtgatgca gtatgaaact atgccaccaa
      421 cgcgttatac cgcgctacac tgggcgggta aggtgagtga tgaagagcgg gcggaaatac
      481 tggcctggat tgcaaaacag cgcgcggaat attacgccag caatgatact gctccggagc
      541 atcgcaatga accggtgcag cccatcccgc aaaaactgcc taccgatgcg caaaaagtgg
      601 cgttgggctt tgcgctgtat cacgatcccc gtttatcggc tgatagcacc atttcatgcg
      661 ctcattgcca tacgttgaat gcggggggcg tcgatggcag aaaaacatcg attggtgttg
      721 gtggcgcagt tgggccgatt aatgcgccga cggtatttaa ctcagtattt aacgttgagc
      781 agttctggga tggtcgtgcg gcaacattgc aggatcaggc aggtggaccg ccgttgaacc
      841 cgattgaaat ggcgtcgaaa tcctgggacg aaattattgc taagctggaa aaagatccgc
      901 agcttaaagc gcagttcctc gaagtttatc cgcaaggttt cagtggcgaa aatattactg
      961 atgccattgc tgaatttgag aaaacattaa ttacgccgga ttccccattt gataaatggt
     1021 tgcgtggaga tgaaaatgcg ctgacggcgc aacagaaaaa aggctatcaa ttatttaaag
     1081 ataataaatg tgcaacttgt catggtggta ttattctcgg cggacgttcc tttgaaccgt
     1141 tggggctgaa aaaagacttt aactttgggg aaattacggc ggcggatatt ggtcgtatga
     1201 atgtgactaa agaagagcgt gataaattgc gtcagaaagt acccggttta cgtaacgttg
     1261 ctttaacggc accgtacttc catcgcggtg acgtgccgac gctggacggg gcggtgaaac
     1321 tgatgctgcg ctatcaggta ggcaaagagc tgccgcagga ggatgtggat gatatcgtag
     1381 ctttcctgca cagtctgaac ggggtgtaca cgccgtatat gcaggataaa caataattaa
     1441 tttgatcgcc cgaacaggaa tgtttgggcg atttttatta cgataataaa gtctgttttt
     1501 aatattatca tgttaaatgt ttatattata aaaagtcgtt tttctgctta ggattttgtt
     1561 atttaaatta agcctgtaat gccttgcttc cattgcggat aaatcctact tttttattgc
     1621 cttcaaataa atttaaggag ttcgaaatgg accagaagct gttaacggat ttccgctcag
     1681 aactactcga ttcacgtttt ggcgcaaagg ccatttctac tatcgcggag tcaaaacgat
     1741 ttccgctgca cgaaatgcgc gatgatgtcg cattccagat tatcaatgat gaattatatc
     1801 ttgatggcaa cgctcgtcag aacctagcca ctttctgcca gacctgggac gacgaaaacg
     1861 tccataaatt gatggatttg tcgatcaata aaaactggat cgacaaagaa gaatatccgc
     1921 aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg catgcgcctg
     1981 cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag gcctgtatgc
     2041 tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca ggcaaaccaa
     2101 cggataaacc aaacctagtg tgcggtccgg tacaaatctg ctggcataaa ttcgcccgct
     2161 actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt atggacccga
     2221 aacgcatgat tgaagcctgt gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga
     2281 cctacaccgg taactatgag ttcccacaac cgctgcacga tgcgctggat aaattccagg
     2341 ccgacaccgg tatcgacatc gacatgcaca tcgacgctgc cagcggtggc ttcctggcac
     2401 cgttcgtcgc cccggatatc gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg
     2461 cttcaggcca taaattcggt ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg
     2521 aagaagcgct gccgcaggaa ctggtgttca acgttgacta cctgggtggt caaattggta
     2581 cttttgccat caacttctcc cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc
     2641 tgcgcctcgg tcgtgaaggc tataccaaag tacagaacgc ctcttaccag gttgccgctt
     2701 atctggcgga tgaaatcgcc aaacaggggc cgtatgagtt catctgtacg ggtcgcccgg
     2761 acgaaggcat cccggcggtt tgcttcaaac tgaaagatgg tgaagatccg ggatacaccc
     2821 tgtacgacct ctctgaacgt ctgcgtctgc gcggctggca ggttccggcc ttcactctcg
     2881 gcggtgaagc caccgacatc gtggtgatgc gcattatgtg tcgtcgcggc ttcgaaatgg
     2941 actttgctga actgttgctg gaagactaca aagcctccct gaaatatc
//
LOCUS       DAEEUE010000218         2965 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.218, whole genome shotgun sequence.
ACCESSION   DAEEUE010000218 DAEEUE010000000
VERSION     DAEEUE010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.218"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..206
                     /locus_tag="KLK84_004133"
     CDS             <1..206
                     /locus_tag="KLK84_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011230.1"
                     /translation="FGASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIA
                     IYLLAGCLISAMTALLMKDNQRS"
     gene            308..1762
                     /gene="amn"
                     /locus_tag="KLK84_004134"
     CDS             308..1762
                     /gene="amn"
                     /locus_tag="KLK84_004134"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HBE2011231.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2105..2821
                     /locus_tag="KLK84_004135"
     CDS             2105..2821
                     /locus_tag="KLK84_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBE2011232.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          833 a          645 c          644 g          843 t
ORIGIN      
        1 tgtttggtgc cagttatcgc tacagtggtg ctggagtcgg ttatcaggtt gccagtgtgg
       61 ttggcggtgg atttacacct tttattgccg ctgcactcat cacttacttt gccgggaact
      121 ggcatagcat cgccatttat ttgctggctg gatgcctgat ttctgcaatg accgctttat
      181 tgatgaaaga caatcaacgc tcgtgatagc ctggcgaaga tcatccgatc ttcgccttat
      241 acttttgttt cacattcctg tgacatacta tcggatgtgc ggtaattgta tggaacagga
      301 gacacacatg aataataagg gctccggtct gaccccagct caggcactgg ataaactcga
      361 cgcgctgtat gagcaatctg tagttgcttt acgcaacgcc attggcaaat atattacaat
      421 tggcgaatta cctgatgaaa acgcccgtaa acaaggtctt tttgtctatc catcactgac
      481 cgtaacctgg gacggtagca caacaaatcc ccccaaaacg cgcgcatttg gtcgttttac
      541 tcacgcaggc agctacacca ccacgattac tcgccctact ctctttcgtt cgtatctgaa
      601 tgagcaacta acgttgcttt atcaggatta tggtgcgcat atctctgtgc aaccctcgca
      661 acatgaaatc ccttatcctt atgtcatcga tggctctgaa ttgacacttg atcgctcaat
      721 gagcgctgat ttaactcgct acttcccgac aacagaactg gcgcaaatcg gcgatgaaac
      781 tgcagacggc atttatcatc caacggaatt ctccccgcta tcgcattttg atgcgcgccg
      841 cgtcgatttt tccctcgcac ggttgcgcca ttataccggt acgccagtag aacattttca
      901 accgttcgtc ttgtttacca actacacacg ttatgtggat gaattcgttc gttggggatg
      961 cagccagatc ctcgatcctg atagtcccta cattgccctt tcttgtgctg gcgggaactg
     1021 gatcactgcc gaaaccgaag cgccagaaga agccatttcc gaccttgcat ggaaaaaaca
     1081 ccagatgcca gcatggcatt taattaccgc tgatggtcag ggtattactc tggtgaatat
     1141 tggcgtggga ccgtccaatg ctaaaaccat ctgcgatcat ctggcagtgc tacgcccgga
     1201 tatctggttg atgattggtc actgtggcgg attacgtgaa agccaggcca ttggagatta
     1261 tgtccttgca cacgcttatt tacgcgatga ccacgttctt gatgcggttc tgccgcccga
     1321 tatacctatt ccgagcattg ctgaagtgca acgtgcgctt tatgacgcta ccaagttagt
     1381 cagcggcagg cctggtgagg aagtcaaaca gcggctgcgt acaggtactg tggtaaccac
     1441 agatgacagg aactgggagt tacgttactc agcttctgca cttcgtttta acttaagccg
     1501 ggccgtagca attgatatgg aaagtgcaac cattgccgcg caaggatatc gtttccgcgt
     1561 gccatacggg acactactgt gtgtttcaga taaaccgtta catggtgaga ttaagcttcc
     1621 cggccaggct aaccgttttt atgaaggcgc tatttccgaa cacctgcaaa ttggcattcg
     1681 ggcgatcgat ttgctgcgcg cagaaggcga ccgactgcat tcacgtaaat tacgaacctt
     1741 taatgagccg ccgttccgat aataagttat gaaccttctt atcagaaata gatgtgaagg
     1801 attaagtaag acatctggca aatagcctgc aataaacgcg ggctgcttgc ttccttgggc
     1861 agatgcgagt tttattatcg tcttaatgat ttccacatat taaaagcaag tatgctttca
     1921 aaacacaatt ataaaaaatc ccgccaacaa cattagtttt tataaaatta aatataagat
     1981 tatggcttta gaatattttt atttctaata gacgagattt ttcctgttat gatataatat
     2041 gccgaattaa cacatgttaa cgatttacca gtaatgtaaa taaattttcg aggagatcat
     2101 tccagtggga cgtaaatggg ccaatattgt tgctaaaaaa acggctaaag acggtgcaac
     2161 gtctaaaatt tatgcaaaat tcggtgtaga aatctatgct gctgccaaac aaggtgaacc
     2221 cgatccagaa ttaaacacat ctttaaaatt cgttattgaa cgtgcaaagc aggcacaagt
     2281 tccaaagcac gttattgata aagcaattga taaagccaaa ggcggcggag atgaaacgtt
     2341 cgtacaggga cgttatgaag gctttggtcc taatggctca atgattattg ctgagacatt
     2401 gacttcaaat gttaaccgta cgattgctaa cgttcgcaca attttcaata aaaaaggcgg
     2461 caatatcgga gcggcaggtt ctgtcagcta tatgtttgac aatacgggtg tgattgtatt
     2521 taaaggaaca gaccctgacc atatttttga aattttgctt gaagctgaag ttgatgttcg
     2581 tgatgtaact gaagaagaag gtaatattgt tatttatact gaacctgctg accttcataa
     2641 aggaatcgcg gcgctaaaag cagctggaat cactgagttc tcaacaacag aattagaaat
     2701 gattgctcaa tctgaagttg agctttcccc agaagattta gaaatctttg aagggcttgt
     2761 tgatgccctt gaagatgacg acgatgtaca aaaagtttat cataacgtcg caaatctcta
     2821 attatatttt aaagaaatct gtctttatgg cagatttctt taatctcata taattcttat
     2881 aaaaaatata atatttaact cgtcatattg cttataaaaa atccccgtcc ccagagaaat
     2941 aatatttatt aaaatggcag gtctt
//
LOCUS       DAEEUE010000219         2926 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.219, whole genome shotgun sequence.
ACCESSION   DAEEUE010000219 DAEEUE010000000
VERSION     DAEEUE010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.219"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..781
                     /gene="lsrF"
                     /locus_tag="KLK84_004136"
     CDS             <1..781
                     /gene="lsrF"
                     /locus_tag="KLK84_004136"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HBE2011233.1"
                     /translation="DWGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTTGLERIDINIA
                     PLFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLN
                     SCAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATR
                     IAAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGV
                     DMGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            805..1095
                     /gene="lsrG"
                     /locus_tag="KLK84_004137"
     CDS             805..1095
                     /gene="lsrG"
                     /locus_tag="KLK84_004137"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HBE2011234.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1134..1892
                     /gene="tam"
                     /locus_tag="KLK84_004138"
     CDS             1134..1892
                     /gene="tam"
                     /locus_tag="KLK84_004138"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HBE2011235.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1896..2810)
                     /locus_tag="KLK84_004139"
     CDS             complement(1896..2810)
                     /locus_tag="KLK84_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HBE2011236.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEEPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          771 a          678 c          781 g          696 t
ORIGIN      
        1 ggattgggga atgcagtcac gcttatcgtg gatatttaat ccgaaaacgg gtaaaactgt
       61 gatgctggcc tttgaccatg gttattttca ggggccgact accggacttg aacgcattga
      121 tataaatatc gccccgctgt ttgaacatgc cgatgtatta atgtgtacgc gcggcatttt
      181 gcgcagcgta gttcccccgg cgaccaataa gccggtggta ctgcgggcgt caggtgcgaa
      241 ctctattctg gcggaattaa gtaatgaagc cgtggcgtta tcgatggatg atgccgtgcg
      301 cctgaacagt tgcgcggtgg cggcgcaggt ttatatcggc agcgaatatg aacatcagtc
      361 gatcaaaaat attattcagc tggtcgatgc cggaatgaaa gtgggaatgc cgaccatggc
      421 cgtgactggc gtgggcaaag atatggtgcg cgatcagcgt tatttctcgc tcgcgactcg
      481 aatcgccgct gaaatggggg cgcaaattat caaaacctat tatgtcgaaa aaggttttga
      541 acggattgtt gccggatgtc cggtacccat tgttattgct ggcggtaaaa aattaccgga
      601 gcgcgagacg ctggaaatgt gctggcaggc tatcgatcag ggcgcttctg gtgtggatat
      661 ggggcgtaat attttccagt ctgaccatcc ggtggcgatg atgaaagccg tacaggcggt
      721 ggttcaccat aacgaaacgg ctgatcgggc atatgaactc tatctgagtg aaaaacagta
      781 actgcggatt taaggagaag aattatgcac gtcacactgg ttgaaattaa cgttcatgaa
      841 gacaaggttg acgagtttat cgaagttttt cgccagaacc acctgggctc tgtacaggaa
      901 gaaggtaatt tgcgcttcga tgtcttacag gacccggaag tgaattcgcg cttttatatt
      961 tacgaagcct ataaagatga agacacagtg gcattccata aaaccacgcc tcactacaaa
     1021 acctgtgtcg cgaaactgga atctttaatg accgggccgc gtaaaaaacg tctgttcaat
     1081 gggttgatgc cgtgaggcta caattggggt aacgcgctga cgggagtaaa aaaatgtctg
     1141 actggaaccc ctctttatat ctgcactttt ccgctgaacg atcgcgtccg gcggtggagc
     1201 tgcttgccag agtgccgctg gagaatgtcg aatatgttgc cgatcttggc tgtggcccag
     1261 gtaacagcac cgccctgctg catcaacgtt ggcctgcggc caggataaca ggcatcgact
     1321 cgtctccggc gatgattgct gaagctcgca gtgctttgcc agactgccag ttcgtggaag
     1381 cagacatccg caactggcaa ccggaacagg cactcgatct gatttttgct aatgcctcac
     1441 tgcaatggct gcccgaccac tacgaattgt ttcctcactt ggtttcttta cttaatccgc
     1501 agggcgtgct ggcagtacag atgccagata actggctgga gccgacccat gtgctcatgc
     1561 gcgaagttgc ttgggaacaa aactacccgg atcgcgggcg tgagccgttg gctggcgttc
     1621 atgcttacta cgatattttg agcgaagccg gatgtgaggt cgatatctgg cgaaccactt
     1681 actatcacca gatgccgtcg caccaggcga ttatcgattg ggtgaccgcc actggattac
     1741 gtccgtggtt acaggatctg accgagagcg aacagcagct ttttcttacg cgctaccatc
     1801 agatgctgga agagcagtat ccactgcaag agaacggaca gatactgctg gcatttccgc
     1861 gtctgtttat tgttgcccgc cgtacggagt aattatcacg tcagctggta atgacgatcg
     1921 ggaagaattt ttgctggaat ttctgcttca tcgttcatct gtaacaggtc aatttcaata
     1981 gcgttgcaga tggcatccag cggtaaatca ttgttttcag taccgaacgg ttcttccagt
     2041 tcttccgcca gacaatccag cgaaataaaa gtgtaggaaa tcagcacaga gataaaaggc
     2101 gtcatgtaat gcaggtccac gaccagcgcg aacggcagca tgatacaaaa cagataaacg
     2161 gtacgatgca aaatcagggt gtaggcaaag ggaattggcg tgtaggcaat gcgctcacag
     2221 cctgccagaa ccgctgaaat atcattaagc cgatcgttga ggctaataaa caggatatct
     2281 gaaagctgtc cattgcggcg ctgaaccgcc aaccattctc ccattattaa caagatacgg
     2341 ttagctggag agttcgaagc cagtacacgc tgaagatctc cagtcttgag ataatgagcc
     2401 agcacttccg cctgtggctg tttgcgtaat gtcatgcgta aacagtgggc gaaggcgatt
     2461 tgcagcctgg caaactccct tacacttgcc gaatccggca atgtcgtttt tacctctcgc
     2521 agtaacgacc gtgaggcaat catcaactgc ccccaaagtt ttcgcgcttc aacgtaacgg
     2581 gcgtacccgg cattattacg aaaaccaaga aaaatggcga tggcgacacc gagaatgctg
     2641 aacggtgcga gggtgaattt gatgcccaga tgcgtgtacc agggcagcat gaatataaca
     2701 gcaatagaaa agagaaaatt gagtagtaag cgcgaggata tcttggataa tactgagccg
     2761 tgccagacaa aaatacggcg cagccagtgt tgttgtggac gaacaatcat ggttatcttc
     2821 aggcgtggaa aagtcgccct attaaacgtg attacgatca cattctcaag acgctactta
     2881 caaattacct agaaccacgg cagcctgacg gctgcaaatt gaaaac
//
LOCUS       DAEEUE010000220         2830 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.221, whole genome shotgun sequence.
ACCESSION   DAEEUE010000220 DAEEUE010000000
VERSION     DAEEUE010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.221"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            306..1472
                     /gene="nhaA"
                     /locus_tag="KLK84_004140"
     CDS             306..1472
                     /gene="nhaA"
                     /locus_tag="KLK84_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HBE2011237.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1538..2437
                     /gene="nhaR"
                     /locus_tag="KLK84_004141"
     CDS             1538..2437
                     /gene="nhaR"
                     /locus_tag="KLK84_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HBE2011238.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(2475..>2830)
                     /locus_tag="KLK84_004142"
     CDS             complement(2475..>2830)
                     /locus_tag="KLK84_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HBE2011239.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
BASE COUNT          666 a          625 c          712 g          827 t
ORIGIN      
        1 tattgacgac aagctggatt atttttgaaa tattggccta acaagcatcg ccgactgaca
       61 acaaattaat tattactttt cctaattaat ccctcaggaa tcctcacctt aagctatgat
      121 tatctgggct tagggtcact cgtgagcgct tacagccgtc aaaaacgcat ctcaccgctg
      181 atggcgcaaa ttcttcaatg gctcgtaaaa aacgaattat tcctacacta taatctgatt
      241 ttaacgatga ttcgtgcggg gtaaaatagt aaaaacgatc tattcacctg aaagagaaat
      301 aaaaagtgaa acatctgcat cgattcttta gcagtgatgc ctcgggaggc attattctta
      361 tcattgccgc tatcctggcg atgattatgg ccaacagcgg cgcaaccagt ggatggtatc
      421 acgactttct ggagacgccg gttcagctcc gggttggttc actcgaaatc aacaaaaaca
      481 tgctgttatg gataaatgac gcgctgatgg cggtattttt cctgttagtc ggtctggaag
      541 ttaaacgtga actgatgcaa ggatcgctag ccagcttacg ccaggccgca tttccagtta
      601 tcgccgctat tggtgggatg attgtgccgg cattactcta tctggctttt aactatgccg
      661 atccgattac ccgcgaaggg tgggcgatcc cggcggctac tgacattgct tttgcacttg
      721 gtgtactggc gctgttggga agtcgtgttc cgttagtgct gaagatcttt ttgatggctc
      781 tggctattat cgacgatctt ggggccatca ttatcatcgc attgttctac actaatgact
      841 tatcgatggc ctctcttggc gtcgcggctg tagcaattgc ggtactcgcg gtattgaatc
      901 tgtgtggtgt acgccgcacg ggcgtctata ttcttgttgg cgtggtgttg tggactgcgg
      961 tgttgaaatc gggggttcac gcaactctgg cgggggtaat tgtcggcttc tttattcctt
     1021 tgaaagagaa gcatgggcgt tctccagcga agcgactgga gcatgtgttg cacccgtggg
     1081 tggcgtatct gattttgccg ctgtttgcat ttgctaatgc tggcgtttca ctgcaaggcg
     1141 tcacgctgga tggcttgacc tccattctgc cattggggat catcgctggc ttgctgattg
     1201 gcaaaccgct ggggattagt ctgttctgct ggttggcgct gcgtttgaaa ctggcgcatc
     1261 tgcctgaggg aacgacttat cagcaaatta tggtggtggg gatcctgtgc ggtatcggtt
     1321 ttactatgtc tatctttatt gccagcctgg cctttggtag cgtagatcca gaactgatta
     1381 actgggcgaa actcggtatc ctggtcggtt ctatctcttc ggcggtaatt ggatacagct
     1441 ggttacgcgt tcgtttgcgt ccatcagttt gacaggacgg tttaccgggg agccataaac
     1501 ggctcccttt tcattgttat cagggagaga aatgagcatg tctcatatca attacaacca
     1561 cttgtattac ttctggcatg tctataaaga aggttccgtg gttggcgcag cggaggcgct
     1621 ttatttaact ccacaaacca ttaccggaca gattcgagcg ctggaagagc gcctgcaagg
     1681 caaattattt aaacgcaagg gacgtggtct cgaacccagc gagctgggag aactggtcta
     1741 tcgctatgcc gataaaatgt tcaccttaag ccaggaaatg ctggatattg tgaactatcg
     1801 caaagaatcc aatttattgt ttgacgttgg cgtggctgat gcactttcca aacgcctggt
     1861 cagtagcgta cttaacgccg cagtggtaga aggcgagccc attcatcttc gctgcttcga
     1921 atccacccac gaaatgctgc tggagcaatt aagtcagcat aaactggata tgatcatttc
     1981 tgactgtccg atagactcta cgcagcagga aggcctgttc tccgtgagaa ttggcgaatg
     2041 tggcgtgagt ttctggtgta caaatccacc accagaaaaa ccgttcccgg cttgtctgga
     2101 agaacggcga cttttgattc ctgggcgacg ttcaatgtta gggcgcaaat tgcttaactg
     2161 gtttaactcc cagggattaa acgtagaaat cctcggcgag tttgatgatg ccgctttgat
     2221 gaaagctttt ggtgcgatgc acaatgcaat cttcgttgcc ccaacgcttt atgcatatga
     2281 cttttatgcc gataaaactg tcgtagaaat tggtcgcgtc gagaatgtga tggaagagta
     2341 ccatgctatt tttgctgagc ggatgattca gcacccggcg gtacagcgaa tctgcaatac
     2401 ggattattct gcgcttttta gtccagcggt gcgttaatcg gcagctcccc caaagttaag
     2461 gtgggggaga tagattagtt gtacattacc acgattttga ctcggctcat tatttgcccg
     2521 cttgagacat tgtttccata tgtacgcggg cgaataaata gaggaatctg attacttcct
     2581 tcatggggat gctgaaaaga gtagtaattg cttttaccat actggatttc attcgtacca
     2641 tcattggaga gttgaaagcc tatttgaccc gcaccgtcct cactgttttc taactcattt
     2701 tttaatatgc tggttcctga ctcctgtatc gtcactggtt caaagtaata ttcaacgtca
     2761 ctgggttcaa aaactaattc accttgctcg ccctggatgc agtcaaatct tatatgatga
     2821 aactgataat
//
LOCUS       DAEEUE010000221         2828 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.222, whole genome shotgun sequence.
ACCESSION   DAEEUE010000221 DAEEUE010000000
VERSION     DAEEUE010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.222"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            105..170
                     /locus_tag="KLK84_004143"
                     /pseudo
     CDS             105..170
                     /locus_tag="KLK84_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            398..646
                     /locus_tag="KLK84_004144"
     CDS             398..646
                     /locus_tag="KLK84_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HBE2011240.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(762..1031)
                     /locus_tag="KLK84_004145"
     CDS             complement(762..1031)
                     /locus_tag="KLK84_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBE2011241.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            1088..1756
                     /locus_tag="KLK84_004146"
     CDS             1088..1756
                     /locus_tag="KLK84_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBE2011242.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            2072..>2828
                     /locus_tag="KLK84_004147"
     CDS             2072..>2828
                     /locus_tag="KLK84_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBE2011243.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTAQPDYHGPRIYFSMSDGQQNTL"
BASE COUNT          863 a          623 c          554 g          788 t
ORIGIN      
        1 ctgactggca ttaccctata gatgcacaaa aaacgggcaa aactacctgg ttcgcaaaac
       61 tgcgtctaaa gttaaactgg gacctcgcga gcaagggtga gacgatggcg ctttacacaa
      121 ttggtgaagt ggcgttgctt tgtgatatta atcctgtcac gttacgcgcg tgacgcaaca
      181 tttttaaaaa tcgacgaaga tcgtccagga gcgccgtttt tcaagggttg gatagatgat
      241 tcgtttacca tgtcgacgaa cttcgacgaa tttcgccgat aaaaagtgtc ccatacatgc
      301 cccattgatg ccccataaac aatatcagga attgacactg gttatccata cagataaaaa
      361 taacacctgt acataactac agttacagga gggtgttatg cgtgttgaga tctgtatcgc
      421 taaagaaaaa atcactaaaa tgccaaacgg tgctgtggat gcgttaaagg aagaattaac
      481 ccgacgcatc agtaaacgtt atgacgatgt agaggtgatc gtaaaagcca ccagcaacga
      541 tggcctttct gttacacgca ccgcagataa ggattctgca aaaacatttg ttcaggagac
      601 tctgaaagat acctgggagt ctgctgacga gtggtttgtt cactaataaa cacgtaaaat
      661 cggtaacggc tggaaatcat tcaatactcg cactatcgaa agtttaccag ccaaccgcag
      721 cacgtcctgc atacgtcgtg tctgcggttt ttctttttcg cttacattgt gtctggttct
      781 tccggccact caatatcagg tgcagttgat gtatcaacac ggttcagcaa cacccgatac
      841 ttcttccagg cttccagcaa cgagttttct tcctccgttg cgatctccag atctacagca
      901 tcctgaagtg gcgcaatatg ctcactgaat ccctggatgt agaactgtgt ggtgacggtc
      961 ttccagccat tcggctcctg ctgtatcgaa gcataccagg ctatttcaat atcgctatgc
     1021 tgcggcagca tttaacccct tgtaattcat cgccataatt gatttaattc acaaataaaa
     1081 ctataatatg gtgaaattaa tgaaaaaaaa cacagatgat ggggctaaaa tttacacacc
     1141 acttacccta aagctttatg actggtgggt tttgggagta tcaaatcggc ttgcatgggg
     1201 atgtcctaca aaggaacacc ttcttccaca ctttctggaa catttaggta acaaccatct
     1261 ggatattggt gttggaactg ggttttacct tactcacgta cctgagagta gtctgatatc
     1321 tttaatggat ttgaacgaag ctagcctgaa cgcggcatct acaagggctg gggaatcaaa
     1381 aattaaacat aaaattagcc atgatgtttt tgaaccttat cctgcggcgt tacatggtca
     1441 atttgattcc atttccatgt tttaccttct tcactgcctg cctggaaata tatctacaaa
     1501 aagctgtgta atacgcaatg cggcgcaggc cttaactgat gatggaactc tatacggagc
     1561 cacaattctt ggcgatggag ttgtgcacaa tagcttcggt caaaaactga tgcgcattta
     1621 caatcagaaa ggcatctttt caaacacaaa agattccgaa gaaggcttaa cacatatact
     1681 ctcagagcat ttcgagaatg ttaaaaccaa ggttcaaggt actgtagtaa tgttttccgc
     1741 ttcagggaaa aaatagcatc caaccgcagc acgttcttgc ttaagacgtg ctacggtttc
     1801 ctttacgatt tggggttgcg actttacctc tattgataat gcattccggc agaacgttca
     1861 aatatgaacg tacgatattt aactaaccga aaaacaaaat atagcacagg cgagacattt
     1921 accatctaag aaaatagtat cgttttttta cagttaaatc aacatcactt ccttaaaatg
     1981 aaaacaataa atttcaatcc tgaaacattt tatttcagct tattattccc cagtatcaca
     2041 ttttatcagg atatccttct gcgaggttat tatgcttcct gtaaataatc cccccctatc
     2101 cactggaaac gtctcttttt acagaactac atcaatcgac aatgttcaca ataattatct
     2161 ctccgaatgg gttgaatgga ctaaaaacag catttccgga gaaaacaggg aaactgcttt
     2221 tacccggctc caattatgtc tggagaacag tgaaacatcg ttggacttat cttgtttagg
     2281 tctcagatct ctaccacgat tgcctgacaa tcttgatgaa attaatgtaa gcaataacca
     2341 actatcaatg ctccccgagc taccaagggc attgaaagag ctgaatgcaa gcagtaatca
     2401 attatctgca cttcctgaat taccagtgtc gctggaatat ataaatgtga gtgataacca
     2461 tttgttcgca cttcctgaat tacctgcgtc actagaatat attaatgtaa gtgacaatca
     2521 cctgtctgta cttccgaggt taccaatgtc attggaatta cttgatgcag ccagaaatgc
     2581 tttggaagta ataccagatt ttccagaaag agatgatcat attataagaa tattctggct
     2641 taatcagaac cggatcacgg caattccgga aagcatactt ggcctcagtt ctgatagcgt
     2701 tgtcaatctt agagaaaatc aactatctcc cagaataatg caaactttgt tacaacaaac
     2761 cgcccaaccg gactaccacg gcccacggat ttacttctcc atgagtgacg gacaacagaa
     2821 tacactcc
//
LOCUS       DAEEUE010000222         2807 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.223, whole genome shotgun sequence.
ACCESSION   DAEEUE010000222 DAEEUE010000000
VERSION     DAEEUE010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.223"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            6..81
                     /locus_tag="KLK84_004148"
     tRNA            6..81
                     /locus_tag="KLK84_004148"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="KLK84_004149"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="KLK84_004149"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="KLK84_004150"
     CDS             complement(299..1057)
                     /locus_tag="KLK84_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBE2011244.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="KLK84_004151"
     CDS             complement(1065..2168)
                     /locus_tag="KLK84_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBE2011245.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..>2807)
                     /locus_tag="KLK84_004152"
     CDS             complement(2178..>2807)
                     /locus_tag="KLK84_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBE2011246.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLATAIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          762 a          738 c          676 g          631 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gttgcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgt agcttgttcc actatctccc cacgatccat aaaaattacc cggtcagcga
      421 cggtacgtgc aaaccccatc tcatgtgtta cacacaacat tgtcataccc gactgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggtt
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc tgaaatctgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttcttagg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt aaaacggtca gatgagggaa gagattgaaa tgctgaaata
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctacgac aatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccggt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt atggacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgttgg ctataacgtg acatgctgaa acaaaagatc
     1141 cagtagatca gcgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaagc cgatgatgat cactaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca acgccagcga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccacctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatact ggtgccttct
     1501 gccataaaca acggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaatgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctcaagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccaggccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgatcg atacaagccc aatcagtaat
     1861 gcgaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgaa tgaagaccca acaggcaccg gcttttgtgc agtcagcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gccgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg tggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatta ctcgcagtgc ctggggaata atgacctggc gtagcgtaac cgggttgggt
     2461 aaccccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agaggtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccattcc gccgcggaag ttaaagcctt gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggagcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaa
//
LOCUS       DAEEUE010000223         2717 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.224, whole genome shotgun sequence.
ACCESSION   DAEEUE010000223 DAEEUE010000000
VERSION     DAEEUE010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.224"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..438
                     /locus_tag="KLK84_004153"
     CDS             <1..438
                     /locus_tag="KLK84_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE2011247.1"
                     /translation="SNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTKAP
                     TVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWESIE
                     MVRRYAHLAPNHLTEHARQIDLILNPSVPNLSQSRNKEGTNDV"
     gene            787..1959
                     /locus_tag="KLK84_004154"
     CDS             787..1959
                     /locus_tag="KLK84_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBE2011248.1"
                     /translation="MHMIEINSLLLITSVILMSLLAVGLFDKISPINLVEHGRNNQID
                     GMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKI
                     ISGDQDWTRLYVSRLLRLTPMFIVSLCLIFIIVGFKSGWRMQVSTEELFVSIMKWLPF
                     TALGMPNINDVKDSFTINAAVTWTLVYEWFFYFSLPVISALIKRKVSIYMVMISAISL
                     FVFILFFSKIHIASFLFGLLAFLLNKSKIVNGIAKAKVTPIIITAIMVFEMTYFKTTY
                     APLPLILCGITFIIIASGCDLYGILRLNITRKLGETTYSVYLLHGIFLYCLMTWIIPN
                     NYTENTFIILVSTTAFLITFTSCLTFKLIETPFIKLTKQTTTLVKELIPTLTNNNQ"
     gene            complement(2013..2636)
                     /locus_tag="KLK84_004155"
     CDS             complement(2013..2636)
                     /locus_tag="KLK84_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE2011249.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSARY
                     THTNIQVTSSGGGKVTVILNGTTFQSAGDYQPSAERPYWITGANCEVVDIGCVFTETT
                     SKVTSASALSVTRSGKINANGSIDVATGVSSVNVVSTGVYDVSFSHPLAAVASGYIVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLI"
BASE COUNT          836 a          564 c          520 g          797 t
ORIGIN      
        1 tcaaaccgcg caattggcgt tgcgctgaat gatactgcat gtcgcgtatt gaaaaaacaa
       61 atcggaaatc atcaccgttg ggtatttgtg tacaaggaaa gctgtaccaa accagacgga
      121 acgaaagcgc caacagtaag gaagatgcgg tatgacgcaa acacagcctg gaaagcggcg
      181 ctgagacggg ctggtattga tgatttcaga tttcacgact tgagacacac ctgggcaagt
      241 tggctggttc aagccggagt cccgttatca gtgttacagg aaatgggagg ctgggagtct
      301 atcgaaatgg ttcgtcgata tgctcacctc gcacctaatc accttaccga acacgcacgg
      361 caaatagact tgattctgaa cccatcggtc ccaaatttgt cccagtcaag aaataaggaa
      421 ggtactaatg atgtgtaact tattgattta aatggtgccg ataatagcag tcgaacctac
      481 gaccttcgca ttacgaatct gtagcaccaa tcataactat ctgttttagc aagcattaac
      541 cgcattcact aagctatagt tgatgacaca aacagaaagt tgatgcatga tgttgccata
      601 tatatgtcac aaatacggca caacgatctt caaacatata gccacacatt cacagaagag
      661 cacaaagcct tgcaatccag tgcaaagctt ggtgtgtctc cgttttgttt catcagttac
      721 acataactat atgatcatta actaaaagtc agtatacttg gagaaatttt aattgcgtaa
      781 tagagcatgc atatgattga aattaacagt ttattattaa taacatccgt gatattgatg
      841 tcgttattag ctgtggggtt atttgataaa atttccccaa taaatcttgt tgagcacggc
      901 aggaataatc aaatagatgg aatgcggggg ttcttagcaa ttttcgtgct tattcatcac
      961 gcagcaattt ggaatggata cttgtcatct ggagtatggg aagcaccttc atcaaatctg
     1021 ttagcaaatt taggccaagt tggtgtgtca ttctttttta tgattactgg ttatctgttc
     1081 ttttcaaaga ttatctctgg agatcaggac tggacaaggc tttatgtatc aagattacta
     1141 cgattaaccc caatgttcat agttagccta tgtctcattt ttatcattgt aggttttaag
     1201 tctggatgga gaatgcaggt atccacagaa gagctttttg tgtcaataat gaagtggttg
     1261 ccattcactg cgctaggtat gccgaacatt aatgacgtaa aagattcatt tactatcaat
     1321 gccgctgtaa catggacact tgtatatgaa tggttctttt atttttctct tccggtaatt
     1381 tccgcgctca taaaaagaaa agtcagtatt tacatggtta tgattagcgc aatatcgcta
     1441 tttgttttca ttttattttt cagtaaaata cacatagcat catttctttt tgggctcctg
     1501 gcatttttgc taaataaatc aaagatagtc aatggaattg ccaaagcaaa agtaacacca
     1561 attataataa ctgcgataat ggtttttgaa atgacgtatt tcaaaacaac ttacgcaccg
     1621 ctaccactga ttctttgtgg aataacattt atcattatcg catcaggttg tgatttgtat
     1681 ggaatattaa gattaaatat aaccagaaag ctaggagaaa ctacctacag cgtttacctt
     1741 ctgcatggga tattccttta ttgcctaatg acgtggatta ttcctaataa ttacactgaa
     1801 aatacattta tcatactggt aagcacaact gcatttctta ttacgttcac atcatgccta
     1861 acttttaaat taatagagac gccattcatc aaattaacaa aacaaaccac aactttagta
     1921 aaagaattaa tacccacatt aacaaacaac aatcaataaa caataaacat aagccgccct
     1981 acatatatgt ccaaatgggg cggcgaatgt atttatatta gtctggtaat gctaaatgca
     2041 aacgaactgg agattcctgc cccgctaagc gtattgcgca gggtcaccct aaatgtatct
     2101 acgccaatat aagtaacatc acaactaact gaatcaggcg cagatatagg tgttatctgc
     2161 acaatataac cgctagcaac tgctgcgagc ggatgagaaa agctaacatc atacacacct
     2221 gtagaaacca cgttaaccga tgaaacacca gtagcaacgt cgatactacc attggcgtta
     2281 atttttcccg agcgtgtgac tgacaatgca gaagccgatg ttaccttact ggttgtttct
     2341 gtaaaaacac acccaatgtc aactacctcg caattagctc ctgttatcca gtatggtcgt
     2401 tctgcacttg gctgataatc ccctgcgctc tggaacgttg tgccgttgag tatgactgta
     2461 acttttcccc cacctgagct tgttacttga atattcgtgt gcgtgtatct tgcactgctc
     2521 acgcgatgaa aatttccacc gtttataacc acagttacag ggcgcgtccc agtattatct
     2581 atagcaaggt ctgccccccc ggcattagcc tcaaagtatg ggttgtttaa taccaaagcg
     2641 cacgaaccgt taaggccatt tatagctaat tgcgcaccac ctgtactgga atccccctga
     2701 gtaccatttc cttcaca
//
LOCUS       DAEEUE010000224         2714 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.225, whole genome shotgun sequence.
ACCESSION   DAEEUE010000224 DAEEUE010000000
VERSION     DAEEUE010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.225"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            6..224
                     /locus_tag="KLK84_004156"
     CDS             6..224
                     /locus_tag="KLK84_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HBE2011250.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            226..591
                     /locus_tag="KLK84_004157"
     CDS             226..591
                     /locus_tag="KLK84_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBE2011251.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            588..1253
                     /locus_tag="KLK84_004158"
     CDS             588..1253
                     /locus_tag="KLK84_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011252.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1253..1618
                     /locus_tag="KLK84_004159"
     CDS             1253..1618
                     /locus_tag="KLK84_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HBE2011253.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2073..2228
                     /gene="hokD"
                     /locus_tag="KLK84_004160"
     CDS             2073..2228
                     /gene="hokD"
                     /locus_tag="KLK84_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBE2011254.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
BASE COUNT          772 a          558 c          696 g          688 t
ORIGIN      
        1 aatgaatggt tacattattt agaaaaaaat atccgcgaaa gagtagaaca acagaattcc
       61 tgtttctcat tctgtttatc gtgttgatga caccgatatc cccgcaaatt tttgtctgga
      121 taatcggaaa aataattgag ccagttattg aattgtataa cgacgtggta tgggcgtcat
      181 tcaacacact gcacaataaa attaatccgt ataaggaaag ctgatatggc actgacgaaa
      241 aaacaacgtg cagaactgcg catgaagttc ggtggtcgct gcgcttattg cggctgcgaa
      301 cttggcgaaa agtggcatgc agaccatgta aaaccggtca ttcgttttga tggaaatatg
      361 cttcaccagg aacgtgacga tatatccaac atggtaccag aatgccaccc atgcaatctg
      421 cacaagcatt gcagtagcct ggaagattat cgacgaatta tcagtgatgg tcgtcgtgaa
      481 ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg gattggttga aatgaaatct
      541 gacccggttg tgttctggtt tgaaaaatat caagaagggg ctacggcatg actactatta
      601 ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgatatt gacgatatag
      661 acggcggtga aattcgtgaa ctggcgctta tcgctctggc atcactggaa gcagagcctg
      721 tagcggaatg cattgttgaa gatgggggca tgtgtattga cgggttcggt gagtatgtgg
      781 gtcactcgct gcctgatgga atgcatgagc tttatgctgc cccgcgaatg cgacaacctg
      841 atggatggaa agccgtagct gtagcgtgga aggtgacgtt tactcaagtt gaccaggaat
      901 ctaatacgtt cactgctata tatttttaca aagcggaagt tgagcgttgg gtacgactgc
      961 ataaagcatg tgattttcgg gcagatataa caccgcttta cgcagcgccg gcagtgccgg
     1021 ttgcagtaaa cgacgacatg gcttacgcat tccatcacgc actgtcagat tcatcgcttg
     1081 gctctgatga aatcgaagaa attaaaaccg gtttgcgtgc tgcctttgcc aacgtcacta
     1141 tccaaccaga gccgatagtg ccggatgaaa tcgggccaaa cgatagtaat acgtttgatt
     1201 atgttgatgg ctggaacgcc tgccgcgctg ccatgcttaa gggagataaa taatgattaa
     1261 tcgaaccaaa ctggagcaca ttctcgaata tgccaggcag cagaaatgta ttgggcaact
     1321 ttgtaaaatt ccaccaggag atatggttga aatcgtggaa atggccatgc gtaaggctgg
     1381 caactctccg gtaactctgg ctggctggat aagctgtagt gatgcagttc ctgctgaata
     1441 ttgcgatgtg attcttctcg atgatctcgg gaatgtattc cccggttcct gggataaggt
     1501 tttttgcccc actcgtggcg ggaataagat ggcttttgtg gacaaagacg gcgttgaagt
     1561 agagagctca actcactgga tgccgctacc ggaaccaccg caggaggtga atcaatgacc
     1621 tggcctgagg cattcaccac ggtaggaatt gcgatggcgg tggcgctggt ggtgtattcg
     1681 atttgccgtt ggggttaaca aacaaaaacc ccggattgat ggtccggggt ttttgaagga
     1741 aacaaaacag aaacagcaat tgccgttacc tgttgttacc atggcaagta aacgtatctc
     1801 aggcgagcgc attgcgccgt tctgacgcag ataaattagc ctggatagaa ggtgctggca
     1861 ataaaaaata gcgttttctt atcggtatcg gtaagattgc tgcgggtgct tgaggctgtc
     1921 tgcctcgggc atgccactgt aaggcagaca gagaaaagcc ccagttaaca ttacgcgtcc
     1981 tgcaagacgc ttaacattaa tctgaggccc aatctatgtc tcacaaatgt aggttagcct
     2041 cttacgtgcc gaaaggcaag gagaagcagg ctatgaagca gcaaaaggcg atgttaatcg
     2101 ccctgatcgt catctgttta accgtcatag tgacggcact ggtaacgagg aaagacctct
     2161 gcgaggtacg aatccgaacc ggccagacgg aggtcgctgt cttcacagct tacgaacctg
     2221 aggagtaaga gaccaggcgg gggagaaatc cctcgccacc tctcatgtgt caggcatcct
     2281 caacgcaccc gcactaaacc cgcttcggcg ggttttttgt tgcgtgctga atacgcaggg
     2341 tgaaaaataa ccatatattt gattatatac acaacaaaaa ataaaagtca ttgtacttgc
     2401 acattaaaca atcaaatata cggcgtgaaa taaatattta tcagattaat atttttgtct
     2461 ctatgtggat ataaccgttt gtacttataa acctgggggc atcgtggaaa aaataaagaa
     2521 actatttagt agcaaatacg cagtcatacg tcgtgatgac ctgtcagtta tagtcgaaat
     2581 ggattacttc cctgaaaccc caaaatcaat aatgtatcgt aatggtcgaa aggcaatttt
     2641 tttaccgatg agggtaagtg acattatggg aaatgataaa ctgctggatg aattgcgagt
     2701 cagagcatcc tgtt
//
LOCUS       DAEEUE010000225         2690 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.226, whole genome shotgun sequence.
ACCESSION   DAEEUE010000225 DAEEUE010000000
VERSION     DAEEUE010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.226"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            230..769
                     /locus_tag="KLK84_004161"
     CDS             230..769
                     /locus_tag="KLK84_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HBE2011255.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(995..1318)
                     /locus_tag="KLK84_004162"
     CDS             complement(995..1318)
                     /locus_tag="KLK84_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HBE2011256.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1604..1798
                     /locus_tag="KLK84_004163"
     CDS             1604..1798
                     /locus_tag="KLK84_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HBE2011257.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEI
                     AEELQNSIQTRFEAESGTGV"
BASE COUNT          857 a          665 c          579 g          589 t
ORIGIN      
        1 aaaaaaaaca ataaaaaaca ccgcaaaaat ggattgttaa caaatgaaaa tactcaattc
       61 gaatctcata acaaacatat catcaacaat aagaaatcac cttctaacat catccaaaaa
      121 tataaaaaca cacccatcac caaacatgga cgtaacaagt catcgtcgtt tattccagaa
      181 agacggaata ggattgagcc aaacaggaaa agcaacacaa cttgtcatta tgtcgcatgg
      241 tggatggaaa gagatctccc atccacaaac actattcacg cgtcaaaaag gcgatggatg
      301 gactgtagtc cccaaaaacc taagaattga tttttataca aaagacaacg acttcacaaa
      361 gggactaagc gttctttcag aggtaaacaa acgccatact gaagctcaaa aagggttaac
      421 accatcacta aacataagca aagatgacct aaaagttcta gcgaacgcgc gcaatatacc
      481 tcaatcaaaa ttagaagaag aaatgatgtc acaagccata tatagaaaag aatacgcaac
      541 aagtaatgag aagataaaaa actatgctct atattaccat gaacaagccc aaaatattat
      601 acaaagacat caagatggct taggaaacaa aaatattgat atagcattta taactgataa
      661 aaatcataag aagcaccttt ctgacatatt taaagtaata aatgaatcag gagtaaaata
      721 tgatgtaatt cactttggcg cttgccgagt aaatagagag gagaaataat catcatttac
      781 cataatccct taattgtacg caccactgaa acgcgctgaa cgcgattctg acgcagacac
      841 cacaaaaata gatgttttac cccggaaaaa caggaaggat gcgtcaggat cgttttcagc
      901 tcgctgcata gctatgcatg aaagcgaatg gagatcactt tgggagctta ccgtgttcat
      961 accgtctgtt ttcaacagtt ttctccccgg gaagctaatc tgccataagc ctggataaca
     1021 gggcacggtg atactccgta atagcgatca gcacctccgc atactccctg tccccgatac
     1081 gcttgccgtc gataaagagt tttttctgta acgcccccag tctcataact tctttcagca
     1141 cccgatcatc agtcagtgag ttaacgttct taccgagagc tgccgcccgt aaaaaaccag
     1201 aaacggtctt gccgcattca gcagcccttt ttctgatttc ctggtcttct tctgccgtca
     1261 gacgaacagg gcgactgata gcccgcctgc gcgtattact tccacttcgt tttgtcataa
     1321 aacatggctc cgtatctgac acggggtgtc ggggcgaagc cctgaccagg gtaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt acacatcctg tcctgtcagc aagttcgaat
     1441 taccgacaaa ggcacggcag tttttttggc agtgacagta ttgtcaggca gaaatacccg
     1501 tcaatgcgtc agacggcaga ataccggcgg taaaacagtc gaaaagtggt acgcgatata
     1561 atcacacaac gcagatgaca gacagaacgg agaaaacaga aagatgaaca agcagcagca
     1621 aaccgtactc aacatggcgg gattcataaa aagccagagc ctgacgctgc tcgaaaagct
     1681 ggacgcactc gatgccgacg aacaggccgc catgtgtgag aagctgcacg aaatcgcgga
     1741 agaactccag aacagcatac agacacgctt tgaagcggaa agtggcaccg gggtataaca
     1801 gtgtatcctg ctccagacag gatgcagaac ggtataccgg tgcgtaagga gaaaatgtca
     1861 cataaggcgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     1921 gcgagcggtg tcagctcact caaaggcggt aatgcggtta tccacagaat caggggataa
     1981 cgccggaaag aacatgtgag caaaaagccg aaggcagggg caaaggccgc gcaggtggcg
     2041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2161 cgctctcctg ttccgaccct gccgtttacc ggatacctgt ccgcctttct cccttcggga
     2221 agcgtggcgc tttctcatag ctcacgctgt tggtatctcg gcccggtgta ggtcgttcgc
     2281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2341 aactatcgtc ttgagtccaa cccggtaaga cacgaattat cgccactggc agcagccact
     2401 gataaccgaa ttagaggagt aagacttgaa gtcatgcgcc tgttaaggct aaactgaaag
     2461 aacaagtttt ggtgactgcg ctcctccaag ccagttacct cggctcaaag agttgatagc
     2521 tctgagaacc ttcgaaaaac cgccttgcag ggcggttttt tcgtttacag gcagcagatt
     2581 acgcgcagaa aaaaaggatc tcaagaagaa cctttaattt tttttcacac ttggtatcct
     2641 actttaaccc ataaaataaa cacttacacc gacacaccct taaacaaaag
//
LOCUS       DAEEUE010000226         2644 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.228, whole genome shotgun sequence.
ACCESSION   DAEEUE010000226 DAEEUE010000000
VERSION     DAEEUE010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.228"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..570
                     /locus_tag="KLK84_004164"
     CDS             <1..570
                     /locus_tag="KLK84_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBE2011258.1"
                     /translation="AELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPG
                     EQTATSILKQRTKNLAPLPHAHQQQNPPQEETVVSIAVDPESPAQYLQRQKPQREEMP
                     VYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKD
                     FEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            571..936
                     /locus_tag="KLK84_004165"
     CDS             571..936
                     /locus_tag="KLK84_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBE2011259.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVC
                     GQLVPGGRLGIKITELGCA"
     gene            933..1616
                     /locus_tag="KLK84_004166"
     CDS             933..1616
                     /locus_tag="KLK84_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HBE2011260.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
     gene            1815..1890
                     /locus_tag="KLK84_004167"
     tRNA            1815..1890
                     /locus_tag="KLK84_004167"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1848..1850,aa:Ile,seq:cat)
     gene            1892..1968
                     /locus_tag="KLK84_004168"
     tRNA            1892..1968
                     /locus_tag="KLK84_004168"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1926..1928,aa:Lys,seq:ttt)
     gene            1971..2046
                     /locus_tag="KLK84_004169"
     tRNA            1971..2046
                     /locus_tag="KLK84_004169"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2004..2006,aa:Thr,seq:tgt)
     gene            2050..2124
                     /locus_tag="KLK84_004170"
     tRNA            2050..2124
                     /locus_tag="KLK84_004170"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2083..2085,aa:Gly,seq:tcc)
     gene            2417..2632
                     /locus_tag="KLK84_004171"
     CDS             2417..2632
                     /locus_tag="KLK84_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBE2011261.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          691 a          581 c          715 g          657 t
ORIGIN      
        1 gctgaattat cctggtgggc ggtccgcaat caggtggcgg acgcgctacc ggaagcggta
       61 ttacgtcgtt cgctggggtt gcgtgcggaa aaaatccgct caatgtaccg tgaaagcgac
      121 atcgtgccgg gagagcagac cgccaccagc atactgaagc agcgcacaaa aaatcttgcg
      181 ccgctgcctc acgcccacca gcaacagaat ccaccacagg aagagacggt ggtcagcatt
      241 gccgttgatc cggaatctcc ggctcagtat ctccagcgcc agaaaccaca acgggaagag
      301 atgcctgtat acacgcgctg ggtaaaaacg cagaaatgca tgacgtgtgg caatcaggca
      361 gatgatccgc accacatcat tggtcatgga ctgggaggga tgggaacaaa ggctgacgat
      421 ttgtttgtta ttccgctgtg tcgtaaatgc cataacgaac tacacgccgg ggtaaaagat
      481 tttgaagaaa aacacggtag tcagttgttg ttgctgattc gttttttaat gcacgcgaga
      541 aattcgggtg ttctgaagtg gaaagcataa atgaccgaac gcatagaatt tgttttgcct
      601 tacccgccaa cggtgaacac ctactggcga cgtcgtggca gcacatattt tgtatcaaaa
      661 gccggtgagc gttatcgccg tgatgtggcg ctaattgttc gccagcagcg actgaaatta
      721 aacctgtccg gaaggctggc gataaagatt attgcagagc caccggataa gcgccgtcgt
      781 gacctggaca atatcctgaa ggcaccgctg gatgcgctga cgcatgctgg actacttata
      841 gacgacgagc agtttgatga aatcaatatt gtgtgcggtc agctcgttcc tggtgggcgg
      901 ctggggataa aaatcacaga actggggtgc gcatgaataa ccagtattta cagtttgtgc
      961 gtgagcagct cattatcgcc accgctgatt tgagtggggc aacaaaaggt cagcttgaag
     1021 cctggcaaga gaatgccatg ttcgatacag ggcgttacag gcgtaaaaaa atccggtacc
     1081 gcgatgaagt gactggaaaa atgataacgc gggataatcc accaatcccg ggaaagcaat
     1141 cgctggcgaa ggggacgtca attcctctgg tcagtccggt tgagttttcg acatcatcgt
     1201 ggcggcgggc tgttctgtct cttgaagagc actataaagc ctggttgttg tggtgttaca
     1261 gcgggagtat ttgttgggaa tatcagatcg cgataacaca gtgggcgtgg aatgaattta
     1321 atgctcaatc cggtaccaga aaaattgcag ggaaaacgca ggaacgcctg aaaaaattaa
     1381 tctggctggc ggcgcaggca gtaaaagcag aactttttgg tggggaaggt tatgaatacc
     1441 aggagctggc attactggcg ggagtgacaa ctaaaaactg gtccaaaaca tttactcgtc
     1501 actgggttgc aatgaaacac atttttcacc gactggatag tgaggcttta ttgtttgtaa
     1561 tgagaacacg ttcaaaaaaa agacggcatt ttcaaagcaa agtgttgcaa aagtagattg
     1621 aaaggcatat attcatgcaa atctgatact ttgccgattt tgtacgtgat ggcaaaagca
     1681 aacaaaaccc gcccacaagc gggttttttg tgccacttat ctcggataga catggtgaat
     1741 gcgctggtgg aggagataag ggtgattttt gaatgcttgc aacattgatt tcgtaacgtt
     1801 attatcctgc gcccggccct ttagctcagt ggtgagagcg agcgactcat aatcgccagg
     1861 tcgctggttc aaatccagca agggccacca accgccacta gctcatcagg aaagagcgtc
     1921 aaccctttaa gttgagtgtg cgaggttcga gtccccggtg gcggtccagt gccgacttgg
     1981 ctcagtaggt agagcaactg acttgtaatc agtaggtcac cagttcgatt ccggtagtcg
     2041 gcaccatatg cgggcatcgt ataatggcta ttacctcagc cttccaagct gatgatgcgg
     2101 gttcgattcc cgctgcccgc tccagtcaga gtctttcagt ctgcgatgat gggaaatccc
     2161 ggagtgactg aaagacgttt aagttatgaa tgatcgcttt tttttgcaaa attgctgtgc
     2221 agaaatacta accttcgggc aggcgatcat tcataagcac tctgctttta ttccgattaa
     2281 ctgtgagtgg tttgttggat agagtgcttt ccttactgta tatatcgttt cgcccgcttt
     2341 tgcggttttt tcttttcaaa tccctttcat ttctcagtgt aaaactacgc catccgttat
     2401 ttgcggaggt gaggctatga aatccatgga caaaatttca acgggcattg cctacggcac
     2461 ctccgcaggc agtgctggct actggttttt acagtggctt gatcaggtca gtccgtcaca
     2521 gtgggctgcg attggtgtac tggggagtct ggttctgggc ttcctgactt atctgacaaa
     2581 tctgtacttc aaaatcagag aagacaagcg taaggctgcg agaggtgaat aatgtcgcca
     2641 tcat
//
LOCUS       DAEEUE010000227         2568 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.231, whole genome shotgun sequence.
ACCESSION   DAEEUE010000227 DAEEUE010000000
VERSION     DAEEUE010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.231"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..489
                     /gene="capU"
                     /locus_tag="KLK84_004172"
     CDS             <1..489
                     /gene="capU"
                     /locus_tag="KLK84_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HBE2011262.1"
                     /translation="EFMLNLLFHLKMNGRQFCWLIVGSGSPELREHLQYQIDSMGMHD
                     DVFIADNVFPAAPVYRVASLVVLPSENESFGMVLAEASAFSVPVVATQIGGIPEVIQN
                     NQTGTLLPASNKHAWMCALNDFFNDPGRFYQMARLAKQDIEERFDINKTALKILTLAK
                     QK"
     gene            494..1444
                     /gene="virK"
                     /locus_tag="KLK84_004173"
     CDS             494..1444
                     /gene="virK"
                     /locus_tag="KLK84_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HBE2011263.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSEYRKRYALLDYIHETTIRSLDAYPVHSEHQNLN"
     gene            1509..2453
                     /gene="lpxM"
                     /locus_tag="KLK84_004174"
                     /gene_synonym="msbB"
     CDS             1509..2453
                     /gene="lpxM"
                     /locus_tag="KLK84_004174"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HBE2011264.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFVTALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKTI
                     ARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP"
BASE COUNT          742 a          489 c          582 g          755 t
ORIGIN      
        1 gaatttatgt tgaatttact gttccattta aaaatgaatg gacgacagtt ctgttggttg
       61 attgtggggt ctggttcacc tgaactgcgg gaacatttac agtatcagat tgacagtatg
      121 ggcatgcatg atgatgtttt tattgctgac aatgtttttc ctgccgcccc cgtatatcgg
      181 gttgccagtc tggtggttct gccttcagaa aacgaatctt ttggtatggt gctggcagaa
      241 gcatcggcat tttctgtgcc tgtagtggcc actcagattg gtggaatccc tgaggttatt
      301 cagaacaacc agaccgggac attgttacca gcaagtaata agcacgcatg gatgtgcgcc
      361 ctgaatgatt tttttaatga ccctgggcgt ttttatcaga tggctcgcct ggcaaaacag
      421 gatatagaag agcggtttga tattaataaa actgcgttaa aaatactcac attagcgaag
      481 caaaagtaac aatatgtttt ctataagtaa cttatcattt atcggtttcc ttaaaaggat
      541 tattttttcc tcagattcac taccggggaa gtgggaacac agaaaatttc ggttcatgta
      601 cattttgcga tgttctataa atccggttgt cagtattcga tattattatg aactgcgttc
      661 cttgccgtgc attgaggata ttctggcaat acaccccacg ttgccagcaa gaattcatcg
      721 cccttatttg cataaagggg ggcgcgcctg gacccgtgga caatatattc ttgagcatta
      781 tcgctttgtg caaaatttgc cagaaaaata ttctaaattt ttgtttccac aaaaatcggt
      841 atcattagtc caattcatcg ggaaagatgg agagaatttt gatatccagt gttctcccag
      901 tggttttgac cgggaaggag agttgatgtt gtcattgttt tacaataaaa cagttattgc
      961 ccggttaaca ttttcagtca ttctgactca aaatggacac attgctttta ttggcggatt
     1021 acagggagcc cctaaaaata ccggacctga tgttattcgg tgcgcaaccc gggcctgtta
     1081 tgggattttc ccaaaacgga ttatttttga agcattttgt gcgttgatga aagcttgcaa
     1141 tatctctgag tgtctggcag tcagtgagca cagtcatgtt ttccggcaat tgagatactg
     1201 gtatcagaaa cgcaaaacct ttgttgcggt ctatagtgat ttctgggagt ctgtagcagg
     1261 aaaaacctgt ggtgactggt acagattacc aacccaggtg attcgaaagc cgctaagcga
     1321 tatagccagt aaaaaacgct ctgagtaccg aaaacgatac gctttgctgg attatatcca
     1381 tgaaactact atccgctctt tggatgcata tcctgtacac tcagaacatc agaatttaaa
     1441 ttaaggtttg atgtattctc tgaataaaac agtaatgata attttggtag aggcattcgc
     1501 actaaataat gaaaaaatat aaatctgagt ttattcctga atttaagaaa aattaccttt
     1561 cccctgttta ctggtctaca tggttccttt tgggaatgat tgcaggtatt tcaatgtttc
     1621 ccccttcatt cagagatcct gtcttggcaa aaatagggcg ttgggcgggc agattgagca
     1681 aaaaagctcg tcgcagggcg acgattaatt tatcgctttg tttcccagaa aagagtgata
     1741 cagaacggga aataattgtc gacaaaatgt ttgtcacagc attgcaatcc atagtgatga
     1801 tggcagaact ggcaattcgt ggtccggaaa agttccagaa acgagtgttc tggaaaggcc
     1861 tcgaaattct tgaggaaatt cgacacaata atagaaatgt gatttttctg gttccccatg
     1921 gctggagcgt ggatattcct gcaatgttgc tggcagctca gggaaaaaaa atggccgcca
     1981 tgtttcatca gcaacgaaat ccagtgattg attatgtctg gaattcagta cggcgtaaat
     2041 tcggggggcg cttacatgcc cgggaggatg ggataaaacc atttattcag tcagtacgcc
     2101 agggatactg gggatattac ctcccagatc aggatcatgg tcctgaatac agtgaatttg
     2161 ctgatttttt tgcgacctat aaagcgacat taccaatcat tggacgtctg atgaacatca
     2221 gtcaggctat gattatacca cttttcccgg tttatgatga aaaaaaacat ctactgacta
     2281 ttgaaattcg gccaccaatg gatgcttgca ttgccagcgc ggacaataaa acgattgccc
     2341 gacaaatgaa caaaacagtg gaaattttgg tggggccaca tccggaacag tatgtctggg
     2401 ttttaaaatt gttaaaaaca cgcaaatcaa acgaagcgga cccgtaccct tgaaattata
     2461 aagtacaagt atttccacta gttgtttgta agcgtacagc ctgaaccgtc tggtcagaat
     2521 ctgacgaatt agacaaagtg gtgtccacca aataagtagt gggaacca
//
LOCUS       DAEEUE010000228         2556 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.232, whole genome shotgun sequence.
ACCESSION   DAEEUE010000228 DAEEUE010000000
VERSION     DAEEUE010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.232"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..314)
                     /locus_tag="KLK84_004175"
     CDS             complement(<1..314)
                     /locus_tag="KLK84_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HBE2011265.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLA"
     gene            complement(330..824)
                     /gene="tssE"
                     /locus_tag="KLK84_004176"
     CDS             complement(330..824)
                     /gene="tssE"
                     /locus_tag="KLK84_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HBE2011266.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(821..1651)
                     /locus_tag="KLK84_004177"
                     /pseudo
     CDS             complement(821..1651)
                     /locus_tag="KLK84_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(1638..2552)
                     /gene="tagK"
                     /locus_tag="KLK84_004178"
                     /pseudo
     CDS             complement(1638..2552)
                     /gene="tagK"
                     /locus_tag="KLK84_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT          545 a          667 c          729 g          615 t
ORIGIN      
        1 gccagatcgc cttcttccgt atcgggatgc aactgcgcca cgctcattga gggcgtgggt
       61 gtgacgtaat tggggctgac cacttccagc aggcgctggg tgaaacgggg gaattcagcg
      121 tcaattttta gctgcgtgcg ggcggtcagg aagctgaagg cctcgaccat tcgctctaca
      181 tacggatcgg caatatccgt tccctgcatg cccagccggg cggcgacttt gggatgacgg
      241 gcggcaaatt cggctcccgt ttcccgcaga tagctcagtt cgcggttgta gtattccagt
      301 agccgtggat ccatcggtct ctctctgaat taaaaaaacg taatgcgact ctgctccata
      361 tccagcgcgc tacgtacccg gaatgcagtc ggatacggct gcgtgaggat ctcgccgcga
      421 atttcaaact gtaatgtgtt atggccactc tggcggcgct catcctgaag tgggatgatt
      481 tgcagagtgg acgctttcag ccggggttca aaccgggtga tggttcgcaa aatggccctg
      541 cggatatcgt cccacttgtg ctcatacaga aagctgcccg ccagtggcgg caggccgtag
      601 ttaagtacgg acgctgccgc gtacggataa cgtgcagcat caatatcatc ttcatggctg
      661 acggtgttga gcagaaatga caaatcccgg cggatgatct ccttaatctg aaccggactg
      721 acgctgatac cgtggtcgcg tttctggttt ggggcgtcgt cacacaggcg gtcaaacagc
      781 gtgggcagca ggtgattgcc tggacgctgg cgggaagcgc tcacgcgtcg cttccttcag
      841 cggtgttaaa ggtacaggtg gacaaatcca gcaggctgat atcgccgtga ccggtgagcc
      901 acaccctctg acccagcgcc tgtaccagtg tctctcccgg accgtcctgc cagaccgttt
      961 cccggcacag acgcagggag tcggcagcct tttcagagcc gctgtagcgg ggaaacagct
     1021 aggcactgtg tgtatcgcca ttgttcaggg tgacgttgac gggtttccag atcaaatcga
     1081 tcagatgcgc aggccgcggc gatttcagcg aacagatttg tgaaaacggc agccagatat
     1141 atgctccgcc cgcgatcagt tcgagtaccg gaccggtacg ggagtcgctg tcgctaatcc
     1201 agtcaaatgc gccgccattc cactgcccgg aagtgtctgt tatggcctca agcgcgcggc
     1261 tgcggtgagc atcagcttcc tgcgcttcac cacgggcgtt acaggcgagg gcggtcagca
     1321 gatcctccat ccaggcgggc ggcgggagaa taacaccggg ccgctgttcg ccctgaaagc
     1381 aggcgtggcg gtatatctca cagcggatca gctcgccaaa tacctgcgct tcgcgagtat
     1441 aatttgcgtc catccgggcg catagctgga tctgttgcag tgcccgcgac cagtcgcctg
     1501 ccacgcacaa taactgaaac aggctgtggc ggcgggaggc ctgcgctggg tgcatcctga
     1561 cctgttgctc tgccatctga atgccctcca ccacggaata atcccgcagc agcgccgaca
     1621 aggtaatcgg tagctcgtta ttgtttgtca tgatatatct ctccatgttg tgccgggttt
     1681 atccggtaat ggctgtcaat gccaatgatg cgatgctctt tccgtgtcag atctggcagt
     1741 acggtgttat gcgctgtttc cggggcgtat tccggggaaa gtaaatgcag gatatcaggg
     1801 ggagtctttt cgacaggcca gatagtttca tcttcgccag tggcatccag tacattgaga
     1861 atagcgtcaa tgcttaaccc accagtgacc atatcctgca atgtctcggc ttcggtgacc
     1921 aggtcacgca atgaatccag atcctgcgag gagggctctt cgttaaaagg aaaaggatca
     1981 gcatggcgat taagatcttg ttcgggagat aacaacgcct cgcggtattc ctgataaagt
     2041 cgggtcaggg catttccttt ttccgcacct tctccggtgg aggaggctcc atgaacagga
     2101 atgatatcga atggattttg tgatgtcaga tgctgctgcc tgaaccaatc cagatccaga
     2161 tattcggtta ttgcttccga cgtgatggca gtctgcgata tcgtttttac tgtgctcagg
     2221 ttcggagatg atggattgcc atgtacaagg cactatgaag ataaccccca ttctatcagg
     2281 tcgccttcat tcagacgcat acggtgatga ggttccacaa tctggtcgtt taccgtacag
     2341 caatattcgg tagactgatt gataatccac catgcttctt cgtggcgggt cagagtgagg
     2401 gcaatcttta catcatcatt caccttaaaa acaggaatcc agggagaggt aacggtgaag
     2461 ataatttgtg ttccttcagt cgcgtaaatt gtgttatcgg cgatcccgtg cgcctgtatt
     2521 ttttggagtc cccacgtaat atttggtttc attgtg
//
LOCUS       DAEEUE010000229         2474 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.233, whole genome shotgun sequence.
ACCESSION   DAEEUE010000229 DAEEUE010000000
VERSION     DAEEUE010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.233"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..392
                     /locus_tag="KLK84_004179"
     CDS             <1..392
                     /locus_tag="KLK84_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBE2011267.1"
                     /translation="IGWSMSPRMTAQLPCDALQMALWRRKRPRNVIVHTDRGGQYCSA
                     DYQAQLKRHNLRGSMSAKGCCYDNACVESFFHSLKVECIHGEHFISREIMRATVFNYI
                     ECDYNRWRRHSWCGGLSPEQFENQNLA"
     gene            complement(496..963)
                     /locus_tag="KLK84_004180"
     CDS             complement(496..963)
                     /locus_tag="KLK84_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBE2011268.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKTNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(1029..1268)
                     /locus_tag="KLK84_004181"
     CDS             complement(1029..1268)
                     /locus_tag="KLK84_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HBE2011269.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1403..2008)
                     /locus_tag="KLK84_004182"
     CDS             complement(1403..2008)
                     /locus_tag="KLK84_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBE2011270.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
     gene            complement(2123..>2474)
                     /locus_tag="KLK84_004183"
     CDS             complement(2123..>2474)
                     /locus_tag="KLK84_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE2011271.1"
                     /translation="AKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEV
                     VVWMTDGCPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKLVELH
                     DKVIGHYLNIKHYQ"
BASE COUNT          736 a          571 c          500 g          667 t
ORIGIN      
        1 ttattggctg gtcaatgtcg ccacgcatga cggcgcaact gccctgcgat gccctgcaga
       61 tggcgctgtg gcggcgtaag aggccccgga acgttatcgt tcacacggac cgtggaggcc
      121 agtactgttc agcagattat caggcgcaac tgaaacggca taatctgcgt ggaagtatga
      181 gcgcaaaagg ttgctgctac gataatgcct gcgtggaaag cttctttcat tcgctgaaag
      241 tggaatgtat ccatggagaa cactttatca gccgggaaat aatgcgggca acggtgttta
      301 attatatcga atgtgattac aatcggtggc ggcggcacag ttggtgtggc ggcctcagtc
      361 cggaacaatt tgaaaaccag aaccttgctt aggcctgtgt ccatattacg tgggtaggat
      421 cacaagacct actgctatca caattactga gagaaaaaaa agcatccgat gattcattta
      481 tgctgtccta aaaaattaac aaaacaatgg gaaaacagga tatgtaagca gaaaagccaa
      541 tgaaattggt acagcataag gcacaccgtt ttcacgaatt gattttcgaa aaaaaagaag
      601 acctgccaga acaaccagag cgccgcaaaa ggtcattgcg acgagaaaat tagccagttc
      661 atgttcatca atagctaacg aaagaacagt taacagcttg acatcaccgc cgccaattat
      721 ccctgcggaa aaaagaaaga agcccacgag caaaacgctc ccggctgcaa ccagattggt
      781 tttttgatgc tgtaaaatga gtgccaaaaa aataagcacg gttaacacca gtaaataacg
      841 atggctaata attctgagta atatatcccc ccatgaaata aaaataagaa agagcgcaac
      901 aaataaaaga tcgactctta gtaaccatat tgctgccatt gaaagactcc atgtaccata
      961 caaggttgct gccagacata ttcctttacg tctgacagcc tctagagagt ttttttattt
     1021 tttataagtt acggattact cgttccggaa atcgttacct gttgaattgc tgcagcgata
     1081 gcgtcgaatg catctttaat tgcgcccagg aagcctgagt tctgatcgcc aaagatgaaa
     1141 gcaagcagag ttgccatagc aacaccaatc aatgcatatt cgattgcagt aacaccacgt
     1201 tcatccttcg cgaaacgcgc aaagcactgt ttagttttac catagtactt agtaataaaa
     1261 gtattcatgt tttcctcgta tattattatt taaattcaaa ccaccagctt catctgcgtg
     1321 tcaccggtat cccatatttt ttggatgtgg ctataattat ccattcccgc tatttagtct
     1381 tcaaaaaaaa ttacatttaa tatcatttat cttgattaag aaatagtaag actaaaaaaa
     1441 caagcgtcgt cagccagcac actgctaaca cggtaagaac gctaataaaa acactccact
     1501 gcttcgttct ataccattca atatttttgt cagtatgttt tattgggagc agctcttcag
     1561 tagagaattc ctggctgtta tcattctcag gaatgtttgc atcagtgttt acttccctgg
     1621 cctcgctatc gttggtatag ctactactgt cttcatcact gtcacgctca ttctcacaga
     1681 catgtttctc gacctttccc agatatctga cgccttttcg cggaatggtt ttaattagag
     1741 aactcgacaa acctgcgacg tctaatgcct ttctaagcat gtaaatctgc ccataataac
     1801 tgctgtcgct taccgaacct ttctgctccc gccaaacctc attgataata tgttgcttat
     1861 cggattctcc ctgtagtaaa aggagtaaaa atcgatagcc gttttcagac agattgatta
     1921 cattgcctgt actggtggtg atacgtcgag acgctgggta aaacttaact ccctgcccta
     1981 aagaaaaata aaaacggtca tcgctcataa gtaccgaccc ctgttttctt cttcacatga
     2041 ctactcatta caactttgta tgaagtaaga tgcaaaatca tattgtttac attaagttaa
     2101 gcaattaggt aatgactcca acttactgat agtgttttat gttcagataa tgcccgatga
     2161 ccttgtcatg cagctccacc aattttgaga acgacagtga cttccgtccc agccttgcca
     2221 gatgttgtct cagattcaga ttatgtcgct caatgcgctg agtgtaacgc ttgctgataa
     2281 cgtgcagctt tcccttcagg cgtgattcat acagcggaca gccatctgtc atccatacca
     2341 cgacctcaaa ggccgacagc aggctcagaa gacgctccag tgtggccaga gtgcgttcac
     2401 cgaagacgtg cgccacaacc gtcctccgta tcctgtcata cgcgtaaaac agccagcgct
     2461 gacgtgattt agca
//
LOCUS       DAEEUE010000230         2433 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.234, whole genome shotgun sequence.
ACCESSION   DAEEUE010000230 DAEEUE010000000
VERSION     DAEEUE010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.234"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            65..1543
                     /locus_tag="KLK84_004184"
                     /pseudo
     CDS             65..1543
                     /locus_tag="KLK84_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            1540..>2433
                     /locus_tag="KLK84_004185"
     CDS             1540..>2433
                     /locus_tag="KLK84_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HBE2011272.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPEQ"
BASE COUNT          630 a          578 c          603 g          622 t
ORIGIN      
        1 tactcgtgag gatttatgtt ctgctggcag tttcgtgatc aacacctcgc gccaggatgg
       61 ctttatgcac tatcgcaatg tggtttgttt gttgtcttgt tcattgtttt tatcgtcggc
      121 atggggatgt cggctttccg cacctgaaca taatatttac cagaaacagg gaaaaggcgt
      181 ggtgtacctg cgcccttata aagaaacaaa tctttctcta cctgacgtga attacaagag
      241 tttgcgtcgg ttacccaatt tattaataga cccgacaaca ctagacgaat gggataaaga
      301 accgcctctg actgatttga caaccgacta cctctatgag ggcgcacagg cctggtatcc
      361 tcattattca tggcatagcg atggtcgcaa tatcctctac gcgggtgaag ttgtgcaaaa
      421 ccctcccggc acacctcccg tcgatgtcgc ctcgttcaag gcatggggtg attttgccgc
      481 agataaacac agtctctatt ttgaaggcaa gcgcaccgat gacaatggtg gtggaaatag
      541 cttggatatc aaaacgctgc atcaggtgga attccggcct ccctgggatc ctgacttact
      601 ggggctgata ttacgtgacg ctaatttctt gtatatcaat gggcatcgcc tcgctgaccc
      661 cgagagtttc cgtgtgctgg cgcagaagtc ctgggatcag cgaggaaagt tttccaccac
      721 gtttaacccc tgcattgccg taccatttgg cccctgggat accctggctc gtacacggac
      781 caaaatcctg ctcaacggcg aacagcttga tgccgacccg gacacctttt ccgtcgtgcg
      841 ctggatgccc ggctcactct tgacctggcg tgataaaaac gggctgcagc gtaaagtcct
      901 cgacaaggaa aatctggcgt gggatgaaga tttaacaaag cactgtctgg atttttctct
      961 gctggaaaag aaagtgttct ggcgtaaagg acctgcttgt aaacaggaag aattacccgg
     1021 actcgatccg gagcagtttc aacccatcag tgatgctgtc gcccagcatc aggactcgct
     1081 ttataccatc atcgaaacag agtctggtga ccgtaagctg gagatcgtga aacttgatga
     1141 tcccaatctt attatcaaca aacgtttcaa cgccgggaaa cgccacggct atttacttac
     1201 gcgtgccgaa gggtagccat accattccgg tttacacgtg tttgaatctg acggaccgct
     1261 gatcttactg gataaccgtt ctccggatga acgcgaagcc catcttaatg accatccctt
     1321 tttgcgcaga tggtatgccc gcgataaccg ctacatttac agctttgatg gcgcgcagct
     1381 ctggcgatac cgcaccgctg atccgaaaca agttcgctta atctggaagg aacaacattc
     1441 gggatatgtc tatggcgtaa attacaaaac gggatatctg gacggaaaaa ttactgatga
     1501 cggcgaattt attcctgccc cgcgcaatga ggcgacaaaa tgaagggtca aataatcaca
     1561 gttgcactca tcctgttggg acacacattt ctgacgcctg ctgtacaggc catagggttc
     1621 gactattaca atgatcacgg tgttatgtct tacggtaaag gttattgggg ggataagaaa
     1681 atcattgccc agtttccgga gatgagcagg gccggtttgc gccttgtgaa aaatatctct
     1741 gggaaaacgg gttatatacc ttccgatgaa ataagcatag aaaatgagga ataccactgg
     1801 gtgacagacg gccgcgttat tctctggcgt ggcaaaatcg ttagcaatcc accgggaaca
     1861 ctcactgtcg atattgccag ctttcaggct atgggccgct tcgcggtcga taaatatagc
     1921 ctctattttg acggacagcg caccgaaagt aatagcggcg cgtcacgtgt tgagctggcg
     1981 acactaaaag ctatcgaagg taactctacc acgctggtgg atagtaaaaa tctctacttg
     2041 tccggtcgac gtcaggggag cagcagtgat gttactgtat tagaaaaaag atggtggggt
     2101 attaatccac gtcttatgag tgtaaacaga aattcatatt ccaatgatct gcttatccgc
     2161 agtgggcaga atatttattt aaatggcgtt caccttacgg cgaatgcaga ctcatttgag
     2221 ataattcgct ggatacctca ttcactgctg gtttttcgcg acaataaggg tctgcatcgt
     2281 tatccctttg gtcaattatc aggcaaagcg atacccgtag atgatgacgt ctcttttgaa
     2341 gtaggggaaa gtcgcgttcg ctggcgtaaa cagctcacgc ccgaccgtca gtggagcaag
     2401 tggatagacc taccaggtat tgaacctgaa caa
//
LOCUS       DAEEUE010000231         2390 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.235, whole genome shotgun sequence.
ACCESSION   DAEEUE010000231 DAEEUE010000000
VERSION     DAEEUE010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.235"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..107
                     /locus_tag="KLK84_004186"
     CDS             <1..107
                     /locus_tag="KLK84_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HBE2011273.1"
                     /translation="QTHLDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            100..1059
                     /gene="ssuA"
                     /locus_tag="KLK84_004187"
     CDS             100..1059
                     /gene="ssuA"
                     /locus_tag="KLK84_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HBE2011274.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1056..2201
                     /gene="ssuD"
                     /locus_tag="KLK84_004188"
     CDS             1056..2201
                     /gene="ssuD"
                     /locus_tag="KLK84_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HBE2011275.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2210..>2390
                     /locus_tag="KLK84_004189"
     CDS             2210..>2390
                     /locus_tag="KLK84_004189"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter"
                     /protein_id="HBE2011276.1"
                     /translation="MMATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPE
                     GVVTAFWTLSASGELW"
BASE COUNT          527 a          653 c          673 g          537 t
ORIGIN      
        1 tgcaaaccca tcttgatacc gcgctagaaa ctttctggca ggcattgcac cgccgcgatg
       61 ttcaggttcc tgaccttctg tctctgcgag gtaatgccca tgcgtaacat cattaaactg
      121 gcgctggcgg gattgcttag cgtctctact tttgcggttg ctgcagaatc ctcgcctgaa
      181 gcgttacgta taggctatca gaaaggcagt attggtatgg tgctggcaaa aagccaccag
      241 ttactggaaa aacgctatcc gcaatcaaaa atctcctggg tggagttccc cgcgggtccg
      301 caaatgttgg aagcgttaaa cgttggcagt attgatctcg gcagtaccgg ggatattccg
      361 ccaatctttg cccaggctgc cggggctgat ttggtatatg tgggcgtcga gccaccgaag
      421 cccaaagccg aagtgattct ggtggcagaa aacagcccga tcaaaaccgt agccgatctt
      481 aaaggtcaca aagttgcctt tcagaaaggt tccagttcac acaacctttt actgcgtgca
      541 ctgcgtcagg ccggacttaa attcaccgat atccagccca cttacctgac gcctgccgat
      601 gcccgcgccg cgttccagca aggtaacgtt gacgcctggg ctatctggga tccctactac
      661 tccgctgcgt tattacaggg cggcgtgcgg gtgctgaaag acggcaccga tctcaatcaa
      721 actggatcgt tttatctggc agctcgtccc tatgcagaaa aaaacggcgc ttttattcag
      781 ggcgtactgg caacctttag tgaggccgat gcgttaaccc gcagccagcg cgaacaaagc
      841 atcgctttac tggcaaaaac gatgggctta ccggcaccgg tgattgcctc ttacttagat
      901 catcgccctc ctaccaccat caaaccggtt aacgccgagg ttgccgcctt acagcagcaa
      961 acggcagatc tgttttatga aaaccgtctg gtgccgaaaa aagtcgatat tcgccagcgc
     1021 atctggcagc ccactcaact ggaaggaaaa caattatgag tctgaatatg ttctggtttt
     1081 taccgaccca cggtgacggg cactatctgg gaacggaaga aggttcacgc ccggttgatc
     1141 acggttatct gcaacaaatt gcgcaagcgg cggatcgtct gggctatacc ggtgtgctga
     1201 tcccaacggg gcgatcgtgt gaagatgcgt ggctggttgc agcgtcgatg atcccggtga
     1261 cgcagcggct gaagtttctt gtcgccctgc gtcccagcgt aacctcacct actgttgccg
     1321 cccgccaggc cgccacgctt gaccgtctct caaatggtcg tgcgttgttt aatctggtca
     1381 caggcagcga tccacaagag ctggcaggcg acggcgtgtt ccttgatcat agcgagcgct
     1441 acgaagcctc ggcggaattt acccaggtct ggcggcgctt attgctgggg gaaaccgtca
     1501 atttcaacgg taaacatatt catgtgcgcg gagcaaaact gctcttcccg ccgattcaac
     1561 agccttatcc tccgctttac tttggcggat cgtcagatgt cgcccaggag ctggcggcag
     1621 aacaagttga tctctacctc acctggggcg aaccgccgga acttgttaaa gagaaaatcg
     1681 aacaagtgcg ggcgaaagct gccgcgtatg ggcgcaaaat tcgtttcggt attcgtctgc
     1741 atgtgattgt tcgtgaaact aacgacgaag cgtggcaggc cgccgagcgg ttaatctcgc
     1801 atcttgatga tgagactatc gccaaagcac aagccgcatt cgcccggacg gattccgtag
     1861 gacaacagcg aatggcggcg ctacataacg gcaagcgcga caatctggag atcagcccca
     1921 atttatgggc gggcgttggc ttagtgcgcg gcggtgccgg aacggcgctg gtgggcgatg
     1981 gtcctacggt cgccgcgcga atcaacgaat acgccgcgct tggcatcgac agctttgtgc
     2041 tttcgggcta cccgcatctg gaagaagcgt atcgggttgg cgagttgctg ttcccacatc
     2101 tggatgtcgc cattccggaa attccccagc cgcagccgct gaatccgcaa ggcgaagcgg
     2161 tggcaaatga ttttatcccc cgtaaagtcg cgcaaagcta aggaacacga tgatggcaac
     2221 gccagtgaag aagtggttat tgcgcgttgc cccctggttt ttaccggtgg gcatcgtggc
     2281 ggtgtggcaa ctggcctcct cggttggctg gctgtcgacg cgtattttgc cttcaccgga
     2341 aggagtggtg acggcgttct ggacgctctc cgccagcggc gaactgtggc
//
LOCUS       DAEEUE010000232         2372 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.236, whole genome shotgun sequence.
ACCESSION   DAEEUE010000232 DAEEUE010000000
VERSION     DAEEUE010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.236"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..823)
                     /locus_tag="KLK84_004190"
     CDS             complement(<1..823)
                     /locus_tag="KLK84_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBE2011277.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
     gene            complement(1423..1671)
                     /locus_tag="KLK84_004191"
     CDS             complement(1423..1671)
                     /locus_tag="KLK84_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HBE2011278.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2026..>2372)
                     /locus_tag="KLK84_004192"
     CDS             complement(2026..>2372)
                     /locus_tag="KLK84_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBE2011279.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          610 a          437 c          576 g          749 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagatgtta atctttgcag ggattgcaga gcatgggatg
       61 acaatgggtt atcactaata tctattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt atgtcaaaat aaccgttcct taaattttcc ggcaggtatg
      181 gaatatcggt taacatattg tcgattgccc atatggagtg aatgttgtga ggtagagggg
      241 ggagttcact aagaagattc cctattgcgc taatctcttg taaagaaaat ggtaaaggtg
      301 gtaaaactgc cagcccatta catgatacat ctaatgtttc cagtagttct ggtaaaacag
      361 gaagagaaca taattgatta tttgagacat gaagctcctt caaagatatg gggagtgtgg
      421 gtagtgtgat tagttgattg tgggacgcat tcaataattt aagtccttga ggcaaagcag
      481 gcagttcaat aagtctgtta tagctgacat taagttgtgt aagggacgca ggtaatgggg
      541 agattaagct taaattattt ttacttatat taattgattt aattcccggg gggatttcag
      601 gtaatgttgt caggcctaat tcagacaagt ccaggttcgt ctcttggttt tgtagacatg
      661 atactagtcg ctgaaaagcg atgtctcgtt gttcttcttg tatgcggtta tttttccatt
      721 cagtccaatg ggttaggtaa ttttcatatg cgcggctagt gtcgattgaa taagtggaga
      781 acgaatttga aattaatctg tgattgttat ttgtcgggag cataaatatc agggcgtatt
      841 ctttatgttg agaggtgttg gattcttttt tatctgcttt gcaatgttat tggttccctt
      901 cagcagtaga caggaacttc tggaggaggc ggatatatag tttgtgtaag catattaact
      961 ccatatgtta tatattgaag aacttctgct ttacactatc ctaaactgga tcggtttaat
     1021 gtaaaaaaac gatacttttt gggggggggg caatttttcc ttaaggtatg aaaaaaggag
     1081 ctgaagctat ataatagcta ttaatgccac tggtatcagt gaatcatgta tataaaaaac
     1141 tgtgttaagg aggctgtcca ttagcggttt tttattcttc tggaaccgcg gcatctttgc
     1201 gtatcttttt gtcttctcac cgtctggtcg gtgttttgct gagactgcta atttcctgtc
     1261 tttgctagtg ctgtactggt actgcccaat catgattggc gggggcgggc ggagttgaga
     1321 ccgcagacac gtcgtatgca agaacgtgct gcggctggct ggtgaacttc cgatagtgcg
     1381 agtattgaat gatttccagc cgttactgat tttacgtgct aattagtgaa caaaccactc
     1441 gtcagcagac tcccaggtat ctttcagagt ttcctgaaca aaagttttag ctgaatcttt
     1501 atcggcggtg cgcgtaacag aaaggccatc gttgctggtg gcttttacga tcacctctac
     1561 atcgtcataa cgtttactga tgcgtcgggt taattcttcc tttaacgcat ccacagcacc
     1621 ggttggcatt ttagtcattt tctctttggc tatgcagatt tcaaaacgca taaaaatccc
     1681 tctgcactgt gtttttatac agtattattt ttaactgtat ggataaacag tgtcaagagt
     1741 gagggaaagt gattttttgg ggcatgcatg gggcaaaaat atttgttgtg gggcattttg
     1801 gggcatgatt gggacacttt gttacatatg aactttgccg attttcatat gacatggcaa
     1861 tccaacgctt tgattattct aaagaaaaca catgctcttg ggcgttcttt agtgattttt
     1921 aaaaatcccg tatcacgcag ttaaagtggc gggcatactc ttcaaggctg gtgatcccca
     1981 accgcaccca tttaggatgt gaccattgcg gtagaccgat gtaaatcata acgcgtgaag
     2041 gatctcttcc acgctacgca cacgggcgat gcgcgggtag atatgattaa tgctgttatt
     2101 gtgttgctcg gcgctggcgg cactacaggc gtcttcggca atcaccagat taaagccaag
     2161 ttcccaggca ttgcgggcgg tggattcaac accgatattg gtcgagatcc cacataacac
     2221 tattgtatcg atacctcggc ggcgcagttg caactccaga tccgtaccgt aaaacgcacc
     2281 ccactgacgt ttgatgattt cgatatcact gtcagttgca cctaatgcag ctggatgttg
     2341 ccaccagttt tcgggcaaca ctttagcggg gg
//
LOCUS       DAEEUE010000233         2190 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.237, whole genome shotgun sequence.
ACCESSION   DAEEUE010000233 DAEEUE010000000
VERSION     DAEEUE010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.237"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..701
                     /locus_tag="KLK84_004193"
     CDS             <1..701
                     /locus_tag="KLK84_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011280.1"
                     /translation="ARAGVPGSDEETRADFENRRRNSVARNARNILEAIRGEILSTVE
                     NVVDVYVTHNPKKTEQKAGVSQYPLTPGSLYVGVYGGSPADIAAAIWRKAPPGIDMNG
                     NTTFTVADKEYDPPYPEYVISWQTLKPVSLHVSVTLKKSDYLPSDITRQVQQSVLDAF
                     NGTDGGLRARVASVVSAGRYYAGIYKTDPEHIDILGLTVSRDGSSWTTAVTFGIDEIP
                     VLDVSDISVKLQEA"
     gene            703..1308
                     /locus_tag="KLK84_004194"
     CDS             703..1308
                     /locus_tag="KLK84_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HBE2011281.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSCWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1295..>2190
                     /locus_tag="KLK84_004195"
     CDS             1295..>2190
                     /locus_tag="KLK84_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE2011282.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRV"
BASE COUNT          533 a          508 c          611 g          538 t
ORIGIN      
        1 acgcccgtgc cggtgtgccg ggcagcgatg aggaaacccg tgcggacttt gaaaaccgcc
       61 gacgtaattc agttgcccgt aatgcccgga atattctgga agccatccgg ggtgaaatac
      121 tctccacggt agaaaacgtg gtggatgttt acgtcaccca taatccgaaa aaaacagaac
      181 aaaaagccgg agtcagtcag tatccattaa cacccggttc gctttatgtt ggcgtgtacg
      241 gcggcagccc ggcagatatc gcggcggcca tctggcgtaa ggctccgccg ggtattgata
      301 tgaatggcaa cacaacgttc actgttgcag ataaggagta cgatccgccg tatcctgaat
      361 acgtgatcag ctggcagaca ctcaaacccg tcagtctgca tgtcagtgtg acgctgaaaa
      421 aaagtgacta cctgccttca gatattaccc gacaggtaca gcaatccgtg ctggacgcat
      481 ttaatggtac agatggtggt ctgcgggcaa gagttgcctc tgttgtctct gccgggcgct
      541 actatgccgg catttacaaa accgatccgg aacatattga tattctgggc cttactgtga
      601 gccgtgatgg ttcgtcatgg acaacggctg tcactttcgg gatagatgag attccggttc
      661 tggatgtgtc ggatatcagt gtgaaactcc aggaggcata acgtgcagaa tgtggctgcc
      721 actgtgcttg cgcagtatgc tgccagcccc cgactcaatg ccctcattaa cagctttaac
      781 gcagcgcttt cccccgacag ttttatcagt gatttttatg gtcttatctg gaacatcgat
      841 accgcagaaa agtatggtct tgatgtctgg ggaaagattg tgggtgtcag ttgctggctg
      901 acggtaaagg acgattttaa ttacctgggc ttcagcgagt ccaggatgga caccccggta
      961 atggatgatc cctgtccgtt taatcaggca ccgttttaca acggaaaatc ggatacccgg
     1021 actgttgacc tgtctgatgc tgtataccgg cggctgatac tgatgaaagc catgtcgaac
     1081 attactgact gttccgttcc ggatattaac ctgatgctga gatttatgtt cggaaaaaaa
     1141 cgccgggctt atgttctgaa taatggcggg ctgaggatga gttacgtctt tgagtccgcg
     1201 ctctcgttgg cagaactggc gattatccag tcgtcgggtg cactgccatc cccgccgggt
     1261 gtttatgttt cagtagtttt aaaggagtcc cgtaatgaag gccagtgata aaccccgcca
     1321 gctggcggtc ccctttgcga gtaccggaga taaaaaccgt atcccggaca aggcgacaca
     1381 gcagaccaga gagagcggta atgctgcgta tgattcaggt tttcctccgg tgaccatgac
     1441 agcggtctca gcgggaggta taccgccaca cggcaaggat tttaacggtc tgatgtacga
     1501 tattaccgca gcaatacggt tcgcccaggc tggcggtttg tacacgtata atgccggttt
     1561 tgcgggggcc attggtggat atgcaaaagg agccattctc gccggagtcg caacaacagc
     1621 ggtctggctg aataccacgg acgataacct gaccgatcct gaaggctccg acagtgcggg
     1681 ctgggtaaat cttcttgagg atccgaaaag gatattcctg cggcagaaga acaatctgtc
     1741 agaccttcag aataaaggga cggcacggga taatcttcag gtttacagta aagagcagtc
     1801 agatcaacgc tatgttcatc gggaaggcga taaaataacc ggagagctga aaatccgtgg
     1861 tgttaatgcg ctgaggattt tcaacgaagc ttttggcctg atttttcgtc gttcggaaga
     1921 gtgcctgcac cttattccca ccagtgaagg tcagggggaa aatggcgata ttggtcccct
     1981 gcgcccgttc accattaatt tgcggacggg tgaaatatcc atgtcgcata aagtgtctgt
     2041 tggcggtggt tcgcaggtca atggtgcgct gggtatcggc gttcagaacg ccctgggggg
     2101 gaattcaatt gttcttggtg ataatgacac cggatttaaa cagaatggag acggtattct
     2161 ggatgtttat gctaatagtc agcgtgtatt
//
LOCUS       DAEEUE010000234         2125 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.239, whole genome shotgun sequence.
ACCESSION   DAEEUE010000234 DAEEUE010000000
VERSION     DAEEUE010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.239"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            42..221
                     /locus_tag="KLK84_004196"
     CDS             42..221
                     /locus_tag="KLK84_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011283.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            273..467
                     /locus_tag="KLK84_004197"
     CDS             273..467
                     /locus_tag="KLK84_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011284.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(1266..1602)
                     /locus_tag="KLK84_004198"
                     /pseudo
     CDS             complement(1266..1602)
                     /locus_tag="KLK84_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          682 a          437 c          436 g          570 t
ORIGIN      
        1 agcgtaaggc actacagcgc attgctttac gagtaataac aatggattta tcaccagaag
       61 acgcaaagtt tttacgagaa ctgcaacacc gtatgctcag agaagccatt atcgaacagc
      121 aaacgggcat gagcgccata gacccattgc gatacaaacg ggcctcatct cctttagttg
      181 acgacaattc caatgcggat aaaacacgtc caccacactg attacatttt tcattagcac
      241 agtggtgtga tgcagcatac gaggaaaacc ggatgaatga gaacaaaata aaaaggctgg
      301 agcaactgtt acaggcacgc caacgggagt ttgccactaa atggggacgt gccgcgctgc
      361 cacatgaacg cctaacctat tttccacttg aagacctcat taaaaataat ggcaaaaaaa
      421 tcaggtctga taagaaacac ttcttagaat gcaatgaaga caaataacaa caatctgcat
      481 attgccacca accacagatg tggcatcgtt atcagcgcac ttgattttct ttatattcac
      541 tgacgagctt gccggggcac ctttgtttgc cggtcattct ggtatcatgc agatatttac
      601 ggcgtacaag cccactagca tgacattgta ataaggcctg agcctaacgc tttaatgtgt
      661 gtcatacact cagtggcgta atcgttgaca atatggtgca tgtaatcgta agcgtccagc
      721 gaaatccatc tggtcataat ctgaagcatc cgacaaagtg gtgtccacca aataagtgat
      781 ctgcgcccaa tatcctgagc cggaactttg acaaaggcca acgaaaaatg ggagcggatg
      841 ttacagagtt ctcggtacag gggaaaagtg gtacctgtcg ccagttctcc gatctgttta
      901 accgggaaat tatctcctac agcttgtcag aaaggtcggt gatggagcat cgttaatacg
      961 atgctatacg atgcattcac agtgcccggc cagaggatgc cccgtcgctg catatggatc
     1021 agggttggca atatcgaatc gcaggctata ggcaaagtta aagccccatg gagtagcaca
     1081 aaatatgccg cacaaaggaa acggtctgaa taacgcagtg atgaagaact tcttcagcac
     1141 actgctaaaa cgcatagtga tcgagcgctg attctggcga acaactgaac taatacatca
     1201 gaatatgcat tatgttaaat aaatatgaaa agatggttta aataccccgt tacttttgac
     1261 ttacactata cggtatcgca tcgtttaata ttcgcaccgg ccagattttt atttctatta
     1321 gttgtcacaa tactgaatgc gtacgaccac agtattctgg ctcctgtgtg gttatgcttt
     1381 aattctgcgt tccgggcaga taagcagttg cttgcaggaa tccttcttgt gttaatgtca
     1441 gttccccttt ttaccagtgc tgatttccac attccgtcca acagagctta tagcctttcc
     1501 ctggattata gcattgtccg gctgaagttc tttttgaata ataatagaag cactgctggc
     1561 agatccagtc cgtttttcat aacccactgt actgataacc ataatctaat cagtagaaat
     1621 tgagtcgaaa ataagcacta ctccatacag gataattaga ggtcagtttg attattcaca
     1681 attcatcatc agcattttct atttctgacg aaatcaacat gaaaataacc atatatgata
     1741 attattataa taacggcttt aattggaata catatattac aacgtattat atataattgg
     1801 tattctggga actatattct caaaatatag tagaaacgag gtatgtttct ggtggaaagg
     1861 acagtgggat taaaaagtaa gaattgataa aaaaaacgcc agcaacacat agtgccggcg
     1921 gagggaatac cccatggaga aaatgtgatg cctagagcat catgaatata taattaaaaa
     1981 tagttagcgt tgtcaatact ttcacaaaaa taattttcgt agtatagaaa gatattttta
     2041 tgcatgacct acctgaattt gctccgggta gaggttataa ataaaaattg aatcacgaca
     2101 aacacaatat tcatagtatg gcgat
//
LOCUS       DAEEUE010000235         2111 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.240, whole genome shotgun sequence.
ACCESSION   DAEEUE010000235 DAEEUE010000000
VERSION     DAEEUE010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.240"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(79..870)
                     /gene="aadA1"
                     /locus_tag="KLK84_004199"
     CDS             complement(79..870)
                     /gene="aadA1"
                     /locus_tag="KLK84_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HBE2011285.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(983..1813)
                     /locus_tag="KLK84_004200"
     CDS             complement(983..1813)
                     /locus_tag="KLK84_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HBE2011286.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
BASE COUNT          516 a          481 c          470 g          644 t
ORIGIN      
        1 aattatgtgc ttagtgcatc taacgcttga gttaagccgc gccgcgaagc ggcgtcggct
       61 tgaacgaatt gttagacatt atttgccgac taccttggtg atctcgcctt tcacgtagtg
      121 aacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata
      181 agcctgtcta gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca
      241 gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga
      301 caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt
      361 taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc
      421 cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag
      481 atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc
      541 tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac
      601 aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa
      661 aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag
      721 caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac
      781 ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt
      841 cgatacttcg gcgatcacca cttccctcat gatgtttaac gtttgaaata aggggcgccg
      901 agcgccagcg aggggagcca aaagcttgct tttggccgtc ccgacttgat tgaagggttg
      961 ggcgattttg ccattagatt ttttataaat ttagtgtgtt tagaatggtg atcgcatttt
     1021 tcttggcttt tatgcttgat gttaaattcg accccaagtt tcctgtaagt gcggacacaa
     1081 aaacatattt atgtcctgat ttgcttataa taaacccttc aaaccatccg ttttgtaagg
     1141 ttctatttgc tgtgaatcct gcaccagttt tcccatacag ttttgtacta ttatccagat
     1201 cttgtagata catgttctct atggtgtttt ctatggctga gtttttaact gggagattgt
     1261 gattaataat tttacgcagg aattgaattt gttcttctgg tgaaattttt aagctacttt
     1321 cgagccatgc ttctgttaat ccgttgtttc tttctttatc tccagagaag tcttgatttc
     1381 cataatcaaa atctttgaga taattcttga ttttatttaa tccaattttt tgggttattt
     1441 cttgcgaaac ccaaacaaca gaaaattgca tccacgtctt tggtgtatga ttgctgttcc
     1501 agatctccat tcctttgggg gttttatccc atttgaatat ggttttctga tctattattt
     1561 ccgcatcaaa tgccataagt gataatgcga tcttgaaagt tgaatctggt gccatttgcg
     1621 ttgcacactt tgctttattg aattgagcaa tttcagcgtt tgtggatgca tcgtaaagta
     1681 aaaaacaacc ttcagttcct tcaaataatg gagatgcaac agtagagata tctgttgatg
     1741 cactggcgct gctgtagata atatttgcaa ttattaaaaa aatagcgaag ttgatatgta
     1801 ttgtgttttt cataataagt attggtttgg taaagggctt aattttaacg gctaacaatt
     1861 aatgaggctc cgggttcgcc caactttgtt ttagggcgac tgccctgctg cgtaacatcg
     1921 ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc
     1981 cgaggcatag actgtacccc aaaaaaacag tcataacaag ccatgaaaac cgccactgcg
     2041 ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac
     2101 ttgcattaca g
//
LOCUS       DAEEUE010000236         2072 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.241, whole genome shotgun sequence.
ACCESSION   DAEEUE010000236 DAEEUE010000000
VERSION     DAEEUE010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.241"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..367)
                     /locus_tag="KLK84_004201"
     CDS             complement(<1..367)
                     /locus_tag="KLK84_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HBE2011287.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFVYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFE"
     gene            complement(406..693)
                     /locus_tag="KLK84_004202"
     CDS             complement(406..693)
                     /locus_tag="KLK84_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011288.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKKIGFKAGFLLLLKNKLIYNLEQD"
     gene            complement(943..1098)
                     /gene="hokD"
                     /locus_tag="KLK84_004203"
     CDS             complement(943..1098)
                     /gene="hokD"
                     /locus_tag="KLK84_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBE2011289.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(1326..1991)
                     /locus_tag="KLK84_004204"
     CDS             complement(1326..1991)
                     /locus_tag="KLK84_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBE2011290.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          533 a          533 c          466 g          540 t
ORIGIN      
        1 cctcaaacga ctttagcaat gccgtatcac gggttctgcg tgtcgcaact gtattcagat
       61 attgctctgc cgcttcgctc agagctggcg tatgttcccg gcctactgat tcacacaggt
      121 aatcaacgaa accggacacc agttttcgtt cgcgaggcgt gatcgcccca ccgaccggag
      181 tccagtaaac gaaacccagt tgcaggagtt tgaaaaaacg cttgtggaat gcgtagttac
      241 gcacacgctt aaagtctgcg tgtatccact cacctatttt gatttgatgc agaaaatcgc
      301 aactctccgg cgtcgccggg agaagtaatc cggaagaggt ttgtttgacc agttgtatat
      361 gtgccatcgg ctttctccgg tggcacggtg ttacacagca ggagttcaat cctgctcaag
      421 attgtagatg agtttattct tcagcaaaag cagaaaacca gccttaaaac caatcttttt
      481 caaaacccgt aatgatgtga caaattcgtc ctcacgcaaa ataaaaccgt ccgtcagaag
      541 tccattacaa aaataaaata acacagcacc gctcttcctt tgttgagatt gcaaacatct
      601 aatgcggcaa tggctgacaa tcgctccatt ctcaacgcgc acagcataga ggccattttc
      661 actaaaaatt tcacgcaatt cttcgatttt catcttcaga atccttccag ataaatagct
      721 ctcccctgtt cggggtccat ccctcttctc cctgcgcgct acttaagtga gtcgattcta
      781 tctgcgaagg tgcgcgaatc aaattcaccg gaaataaaca acaaaaaacc cgccgaaacg
      841 ctgagagatc acagcgatgg gcgggttaag tgcgggtgcg ttgaggatgc ctgacacatc
      901 agaggtgggc ggggatggga tcagctcccc acccggtcac tcttacttcc tggattcgta
      961 gtctacgaag acagcgacct ccgtctggcc ggttcggatt cgtacctcac agaggtcttt
     1021 cctcgttacc agtgccgtca ctatgacggt taaacagatg acgatcaggg cgattaacat
     1081 cgccttttgc tgcttcatag cctgcttctc cttgcctttc ggcatgtaag aggctaacct
     1141 acatgtgcaa agcatgaaat tggcctcaga ttaatgttaa gcgtcttgcc ggacgcgtaa
     1201 tgttaactgg ggcttttctc tatctgcctt ttggtgttca tgcctgaagc agatagcctc
     1261 aagcacccgc agtcattcta cttacctcgc caatatgaaa tcaatcagaa aggtgcccca
     1321 taaaatcact ccttctcttc tttaccgtag tggagttgac caattttgat aagagggcgt
     1381 ccctgagatt tgcggtgtag attggtatcg cgcagagaat acacacagcc acaatattcc
     1441 tgctgataga atttttcgcg cttgctgatt tcaatcatac gggacgagcc gccctgcttg
     1501 cgccagttat aatcccagta caccatgccc ggataatgcg cgacggctcg ccgcccacag
     1561 tcgttaacct gctgcatatt tttccagcgt gaaatgccca gtgaactgct gatcacactg
     1621 aaaccatttt cagcggcgta cagcgctgtc cgctcaaaac gcatgtcaaa acacatggtg
     1681 caacggatcc ctcgttcggg ctcccattcc attcctttgg ctcgttcaaa ccagttgtct
     1741 gtgtcgtaat cagcatcaat aaacggcacg ccgtgttgtt cagcaaagcg aatattctca
     1801 tccttacgaa ttaaatactc tttctgagga tgaatgttcg ggttgtagaa aaagatggtg
     1861 tagtcgattc ccgaggcctg aagcgcctcc atcacctcac cggaacatgg agcacagcaa
     1921 gagtgcagta gtagtttgtt tgccccgttt gggagctcca atttgggccg tttgaaatca
     1981 gcaactgtca taaatatgtt tattgaggtc ataaaaatat caaagagtgt agcattagaa
     2041 cggggctatc ggaaacagat gtgtgactgc tc
//
LOCUS       DAEEUE010000237         2033 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.242, whole genome shotgun sequence.
ACCESSION   DAEEUE010000237 DAEEUE010000000
VERSION     DAEEUE010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.242"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..363)
                     /locus_tag="KLK84_004205"
     CDS             complement(<1..363)
                     /locus_tag="KLK84_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE2011291.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(430..1644)
                     /locus_tag="KLK84_004206"
     CDS             complement(430..1644)
                     /locus_tag="KLK84_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HBE2011292.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          634 a          493 c          412 g          494 t
ORIGIN      
        1 cagaccgata cgcattaaaa taaagtggct aaaagaatta accattcctg ttaatgcctg
       61 cattaatgac cagaatatta agcctgcgcc taaaactatt cgtgggccaa agcggtctag
      121 caaaataccg gaaggtagtt gtgaaaaacc ataagacaga gaaaatgcag aaagcaaaac
      181 accaaactct gtcgccgata atcctaactc gccacgaatc gcttcgcctg ccacgctcaa
      241 tgacgaacgg tcgagaaaat taacgatccc cgccataaat aataaaacca gggtcactgt
      301 ctgaatacga cgaatccgtt gcggtttatt acccgcaaca ctggttgaca catcaacgtc
      361 catttttagc tccttgagcg gaattaatcg atttgatgac tattcagaaa ataattaagc
      421 gaaccgttat taccagttca acaatgtgcc atcttccagt cgtgcgacgg ggagatactc
      481 cgggtcatac ggatatttcg ccgccagttt ctcatcgaaa gaaataccca gccccggttc
      541 atcgcctggg tgcatataac cttcctcgaa gcgccagctg tacgggaaaa cttcaagcat
      601 ttgttcggaa tagcccatgt actcctgaac gccaaaattt ggtacccata ggtcaaaatg
      661 cagcgccgcc gcatgacaaa caggcgataa atctgaaggg ccatgcgaac cggtacgtac
      721 ctgataaagc gatgcaaaat cggcaatccg ccgcatcccg gtaatgccgc ctgcatgggt
      781 gatcgtcgtg cgaatatagc caatcagctg ctcttcgatt aactgcttgc agtcccagat
      841 actgttaaac acttcaccga ccgcaatcgg cgtcacggta tgctggcgaa tcagacgaaa
      901 acattcctgg ttctcggcag gcgtcggatc ttccatccag aacaggcggt aatcctcaat
      961 gctcttccca aagcgcgccg cttcgatggg ggtcaggcga tggtgcatgt catgcaacag
     1021 atgttcgtta aaaccgaact tattacgcac cgcttcgaat aacttcggcg taaaatcgag
     1081 atacttttct gaagaccaca actgctcttc tggccacagg cctttggttg ccggttcata
     1141 cgccagtcct ttgcccttcg ccaggccata agtggtttgc atacccggta caccgcactg
     1201 cacacgaatc gccttaaagc ccatctgctg gtgctttgca tagtcttcaa ggacttcatc
     1261 aatcgtacgc ccggtggtgt ggcaataaac catcacgcct tcacgagaag caccaccaag
     1321 cagttgatac aatggcatat ttgccgcttt agctttgata tcccacaacg ccatatcaat
     1381 ggcagaaatg gctgacatcg tgacggggcc acgtcgccag taagcacctt tataaaagaa
     1441 ttgccagata tcttcgatac ggctggcatc gcggccaatt agttgtggac aaagatgatc
     1501 tttcagataa gaagcgaccg acaattcgcg accatttaac gtggcatctc ctaatccaca
     1561 aagtccgctt tcagtggtta ttttaagcgt aacgaaatta cgccctgggc aagtaacaaa
     1621 aacatctgca gcaataattt tcattattgc aatctcctgt gttgtaaata gacgtttcaa
     1681 acctaatttt acgcctacta ccatacaaga gagaaaaagc gattattcaa ccgtgatcac
     1741 attaaaaaca aaaatttcca ccgaggagaa aaaataccct caaaaaccac ctgaataatt
     1801 aaccattatt gttaaaacaa cacgttaaat aatcacataa caaactccat taatcattga
     1861 aaatgagcac agaaatagaa attaattata ttcaattaat taaaatgtgt attgcttgat
     1921 gtatttaaaa atattatttt catggtgaga gtggaggtaa agaagacaca ataataaaaa
     1981 ggaatgtgac aaataatggt tctggttggg cgttaaattt aaggttacca gaa
//
LOCUS       DAEEUE010000238         2032 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.243, whole genome shotgun sequence.
ACCESSION   DAEEUE010000238 DAEEUE010000000
VERSION     DAEEUE010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.243"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..131
                     /locus_tag="KLK84_004207"
     CDS             <1..131
                     /locus_tag="KLK84_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBE2011293.1"
                     /translation="YYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGTFYTPQGS"
     gene            complement(831..905)
                     /locus_tag="KLK84_004208"
     tRNA            complement(831..905)
                     /locus_tag="KLK84_004208"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(870..872),aa:Gly,seq:tcc)
     gene            complement(909..984)
                     /locus_tag="KLK84_004209"
     tRNA            complement(909..984)
                     /locus_tag="KLK84_004209"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(949..951),aa:Thr,seq:tgt)
     gene            complement(987..1063)
                     /locus_tag="KLK84_004210"
     tRNA            complement(987..1063)
                     /locus_tag="KLK84_004210"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1027..1029),aa:Arg,seq:tct)
     gene            complement(1065..1140)
                     /locus_tag="KLK84_004211"
     tRNA            complement(1065..1140)
                     /locus_tag="KLK84_004211"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1105..1107),aa:Ile,seq:cat)
     gene            complement(1317..2006)
                     /locus_tag="KLK84_004212"
     CDS             complement(1317..2006)
                     /locus_tag="KLK84_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HBE2011294.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQKA
                     AFSKQSIAKVD"
BASE COUNT          542 a          533 c          432 g          525 t
ORIGIN      
        1 cttactacac acgcaaaatg agtcagggaa aacgacacaa tcaggcgctt atcgccctgg
       61 cgagacgacg ctgcgacgtt ctgttcgcca tgatgcgcga cgggactttt tataccccgc
      121 aggggtcata acatgcttga caacttaata ggggcccccc ccgacagacc tgtgctatcg
      181 gaaagaaaaa aggccatcag caaaactctg atgacctgaa tcacctttac caatattgta
      241 tgaaaaaaca cgcacgacta attgacaata attttcattt ccattaaaaa agatcgcgta
      301 gcattcttaa ttcatgagga actttacccg ccagcaatct gagtagcgaa agctgttcat
      361 ccccaatgtt tttgctggcg ggtccttttt tctacggtcc tcctccccgg aggggggcat
      421 ttcattattt ttctaatcgt tactggtgag aaggcggaag aaaaagccag ttcttcgccc
      481 ctaaataaca gcatgattcc agtccatgca aaatctgatt tccagctcac acataccgag
      541 cgctgtacaa aaaaccgcca ggcaaggcag ttaagagtgt gtccccgggg tttgcttaga
      601 atattttgta agttgtccga agagatattt acaacaccag aatgatgaat catcagtccc
      661 ctgccagagg ttcactacgt aacctgaccg acaatgcacc attctggtgc tgaaaaaaga
      721 gcactggaac tgcaacaaaa caacatcaca gaacaggaga tggtaaggag tcagacattc
      781 ccacacaata ttgtgtcagt gcaaataaca accttcgtct caaatctcgc tggagcgggc
      841 agcgggaatc gaacccgcat catcagcttg gaaggctgag gtaatagcca ttatacgatg
      901 cccgcatatg gtgccgacta ccggaatcga actggtgacc tactgattac aagtcagttg
      961 ctctgcctgc tgagctaagt cggcgctggc ccaccaccga ggactcgaac ctcggaccgt
     1021 caacttagaa ggttgatgct ctatccggat gagctagtgg tggttggtgg cccttgctgg
     1081 acttgaacca gcgacctggc gattatgagt cgctcgctct caccactgag ctaaagggcc
     1141 ggaggcagaa taataaccat atgtcatcac atctgcaaac tcatctgacc accagcacgt
     1201 ttaacgtcct gtgccatttt tcaggcataa aaaaaccgct cggcggcggg ttttgcttac
     1261 tttaccatcg cgtacaaaat cggcaaagta tcagatttgc atgagatata tgccttttaa
     1321 tctacttttg caatactttg ctttgaaaat gccgcttttt gttctgaacg tgttctcatc
     1381 acaaacaata aagcctcact atccagtcgg tgaaaaatgt gtttcattac aacccagtga
     1441 ccagtaaatg ttttggacca gtttttggtt gttactcccg ccagtaatgc cagctcctga
     1501 tattcataac cttccccacc aaaaagttct gcttttaccg cctgcgccgc cagccagatt
     1561 aattttttca ggcgttcctg tgttttctct gcaattttcc tagtgccaga atgagcctta
     1621 aactcttccc acgcccagtg ggttatggtg atctgatact cccagcaaat acttccgctg
     1681 taacaccaca acaaccaggc tttatagtgc tcttcaagag acagaacagc ccgccgccac
     1741 gatgatgtcg aaaacgcaac ctgactgacc agaggaattg acgccccctt cgccagtgat
     1801 tgttttcccg ggattggtgg attatcccgc gttatcattt ttccggtcac ctcatcgcgg
     1861 taccggattt ttttacgcct gtaacgccct gtatcgaaca tggcattctc ctgccaggct
     1921 tcaagctgac cttttgtcgc cccactcaaa tcagcggtgg cgataatgag ctgctcacga
     1981 acaaactgta aatactggtt attcatgcac actccagctc tgtgattttt at
//
LOCUS       DAEEUE010000239         2031 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.244, whole genome shotgun sequence.
ACCESSION   DAEEUE010000239 DAEEUE010000000
VERSION     DAEEUE010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.244"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..>2031
                     /gene="sat"
                     /locus_tag="KLK84_004213"
     CDS             <1..>2031
                     /gene="sat"
                     /locus_tag="KLK84_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HBE2011295.1"
                     /translation="GMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTTHGIA
                     SVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTGTQEK
                     ITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGKESIV
                     NWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGNGKVI
                     LNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEAVLAI
                     NNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTMQGHA
                     TEHAIFRSTASHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQPDWKT
                     GVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNNGFDF
                     KQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAKVIAQ
                     NSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMASVTG
                     NIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSMNNAI
                     WHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQA"
BASE COUNT          745 a          358 c          401 g          527 t
ORIGIN      
        1 acggcatgat gatttataaa aatgataaaa catttcgtaa tcttgaaata ttcggagaca
       61 gcggctctgg agcatactta tatgataaca aactagaaaa atgggtatta gtcggaacaa
      121 cccatggtat tgccagcgtt aatggtgacc aactgacatg gataacaaaa tacaatgata
      181 aactggttag tgagttaaaa gatacctata gtcataaaat aaatctgaat ggcaataatg
      241 taaccattaa aaacacagat ataacattac accaaaacaa tgcagatacc actggtactc
      301 aagaaaaaat aactaaagac aaagatattg tgttcacaaa tgggggaaat gttctgttta
      361 aggataattt ggattttggt agcggtggta ttatctttga cgaaggccat gaatataaca
      421 taaacggtca gggatttaca tttaaaggag caggaattga tatcggaaaa gaaagcattg
      481 taaactggaa tgcattgtat tccagtgatg atgttttaca caaaataggc cccggtactc
      541 tgaatgttca aaaaaaacag ggggcaaata taaagatagg tgaaggaaat gttattctta
      601 atgaagaagg aacatttaac aatatatacc ttgcaagcgg aaatggtaag gtaatactaa
      661 ataaagataa ttcccttggc aatgatcaat atgcggggat attttttact aaacgtggtg
      721 gcacgctaga tttaaatgga cacaatcaga cttttactag aattgccgcc actgacgatg
      781 gaacaacaat aactaactca gatacaacga aagaagccgt tctggcaatc aataacgaag
      841 actcctacat atatcatggg aacataaatg gcaatataaa actaacgcac aatattaatt
      901 ctcaggataa gaaaactaat gcaaaattaa ttctggatgg tagtgtcaac acaaaaaatg
      961 atgttgaagt cagtaatgcc agtcttacca tgcaaggcca tgcaacagag catgcaatat
     1021 tcagaagcac agcgagtcat tgctccctgg tatttctttg tggaacggac tgggtcaccg
     1081 ttttgaaaga aacagagagt tcatataata aaaagttcaa ttctgattac aaaagtaata
     1141 atcagcagac ctcatttgat cagcctgact ggaaaaccgg ggtgtttaaa tttgatacat
     1201 tacacctgaa caatgctgac ttttcaatat cacgcaatgc caatgttgaa ggaaatatat
     1261 cagcaaataa atcagctatc acaatcggcg ataaaaatgc ttacattgat aatcttgcag
     1321 ggaaaaatat tactaataat ggttttgact tcaaacaaac tatcagtact aatctatcca
     1381 taggagaaac taaatttaca ggtggcatca ctgcacataa cagccaaata gccataggtg
     1441 atcaagctgt agttacactt aatggtgcaa cctttctgaa taatactcct ataagtatag
     1501 ataaaggagc aaaagttata gcacaaaatt ccatgttcac aacaaaaggt attgatatct
     1561 ccggtgaact gactatgatg ggaatccctg aacagaatag taaaactgta acgccgggtc
     1621 tccactacgc tgctgatgga ttcaggctga gtggtggaaa tgcaaatttc attgccagaa
     1681 atatggcatc tgtcaccgga aatatttatg ctgatgatgc agcaaccatt actctgggac
     1741 agcctgaaac tgaaacaccg actatatcgt ctgcttatca ggcatgggca gagactcttt
     1801 tgtatggctt tgataccgcc tatcgaggcg caataacagc ccccaaagct acagttagca
     1861 tgaataatgc gatctggcat ctaaatagcc agtcatcaat taatcgtcta gaaacaaaag
     1921 acagtatggt gcgttttact ggtgacaatg ggaagtttac aacccttaca gtggacaacc
     1981 ttactataga tgacagtgca tttgtgctgc gtgcaaatct ggcccaagca g
//
LOCUS       DAEEUE010000240         1969 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.245, whole genome shotgun sequence.
ACCESSION   DAEEUE010000240 DAEEUE010000000
VERSION     DAEEUE010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.245"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..1037)
                     /gene="ipaH3"
                     /locus_tag="KLK84_004214"
     CDS             complement(<1..1037)
                     /gene="ipaH3"
                     /locus_tag="KLK84_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HBE2011296.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLSQQTAQPDYHGPRIYFSMSDGQQNTL
                     HRPLADAVTAWFPENKQSDVSQIWHAFEHEEHANTFSAFLDRLSDTVSARNTSGFREQ
                     VAAWLEKLSTSA"
     gene            1779..>1969
                     /locus_tag="KLK84_004215"
     CDS             1779..>1969
                     /locus_tag="KLK84_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBE2011297.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLR"
BASE COUNT          544 a          345 c          489 g          591 t
ORIGIN      
        1 gcagaggtac tgagtttttc cagccatgca gcgacctgtt cacggaatcc ggaggtattg
       61 cgtgcagaga cggtatcgga aaggcggtca aggaacgcgg aaaaggtgtt ggcgtgctct
      121 tcatgttcaa aagcatgcca tatctgtgat acatcagatt gtttgttttc cgggaaccat
      181 gctgtcacgg catcagccag ggggcgatgg agtgtattct gttgtccgtc actcatggag
      241 aagtaaatcc gtgggccgtg gtagtccggt tgggcggttt gttgcgacag agactccctg
      301 atccgtgagg acagaggatt gtcttcgagg atgatagtgc aggtcggatc aaggctaagt
      361 atattttccg gaatgtgtgt gatgcgattc tcgcggcacc ggaaaaatat ctcggtttcc
      421 tctgagtgat gatttcttac aggtacggct ggtaggcttt ccagaagatt agtacttaca
      481 tcgagcgctt ccagtgattc aggtaactca gggagaaatg tcagctggtt atttcttact
      541 gagagcactt ccagcgatgt aggtaattca ggaagcatgg ttagctgatt gttatctgca
      601 ttaatatatt ccagcaatgc aggcaattca ggaagcatgg ttagttggtt gttatctaca
      661 tcaagatgtt tcagagatgc gggtaattca ggaagtgttg acaggcgatt gtcacaggcg
      721 tcaaggtatt ccagcgatgc tggcaattct ggtaatgata ttagggcatt ctgagtaatt
      781 tccagaacag tgatttgagg tggtaagttg tcaggtagcg aggacagatt taaacgattc
      841 agttgaagct cactgaactg attgatgaga cattctttaa gtagggagac cgcttcattc
      901 cgattttcac cggggagcgc ttgtttttcc catttatccc atgctgaaaa gtaatcagca
      961 tatgtaccgg aaatagtgtt ataaaaagaa ttttgggaca atgaaaagtt attatttatc
     1021 ggtaacatga tggacattca gttaaagccg atggacaaat ggctttgtta aaagaaataa
     1081 gtaggagttt ttatgaagtt cattccatga aatgaacttc agcacttttt tattataaaa
     1141 ggacaaacaa aattggctgt aaaaaaacga tactgtttca gttaactgtg aatagcttgc
     1201 ttgcaccgta ttgttcttgt ttttcggtta gttaaacatc gtacttcata tttgaacgtt
     1261 ctgccggaat gcattatcaa tagaggtaaa gtcgcaaccc caaatcgtaa aggaaaccgt
     1321 agcacgtcgt atgcaagaac gtgccacggc tggctgatgg atgttcgata gcgcgagttt
     1381 gaatgaaaat cagccggaga tgattttaca taattgctac ggaattattc aatacaggaa
     1441 ttgcttgcgt ctgcatggat tgacctgaaa tattcccgaa aatttctcta aaaaactcga
     1501 aaaaaatggt aactggttga atgtattaat atgcaatggt acgtgtcagg gattaaaaga
     1561 tgaacgtaaa tttattcaac gcattaattt taaagggttt tattgtttgt tgacgaaaac
     1621 aggaatcatg ttcggtctct ttttatctgt taaaagccag aagcatttcc ttcgctgact
     1681 ttatagtcaa ccataacaca cactctactg tctgagtcca gcgtttttta acattcttgt
     1741 taagattatg tgatctttag cgcgggagga aaatattgat gaaacagcct gcgcccgttt
     1801 atcagagaat tgcgggtcat caatggcgac atatctggct ttctggcgat atacacggtt
     1861 gtcttgagca gttgcgccgc aaattatggc attgtcgttt tgatccgtgg cgagatttac
     1921 ttatctcagt gggagacgtt atcgatcgtg ggccgcaaag tttacgttg
//
LOCUS       DAEEUE010000241         1919 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.246, whole genome shotgun sequence.
ACCESSION   DAEEUE010000241 DAEEUE010000000
VERSION     DAEEUE010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.246"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(226..429)
                     /locus_tag="KLK84_004216"
     CDS             complement(226..429)
                     /locus_tag="KLK84_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011298.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(431..586)
                     /locus_tag="KLK84_004217"
     CDS             complement(431..586)
                     /locus_tag="KLK84_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HBE2011299.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(789..1196)
                     /locus_tag="KLK84_004218"
     CDS             complement(789..1196)
                     /locus_tag="KLK84_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE2011300.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1273..1500
                     /locus_tag="KLK84_004219"
     CDS             1273..1500
                     /locus_tag="KLK84_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBE2011301.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1484..1906
                     /locus_tag="KLK84_004220"
     CDS             1484..1906
                     /locus_tag="KLK84_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011302.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
BASE COUNT          468 a          414 c          492 g          545 t
ORIGIN      
        1 ccagaggatt acgaagggct tcataaatat atgctctcac taggattcag gagaacgatc
       61 ccccatggag atggttcgta taatcaactc ccggacggaa cttatgtttc cgaaaagggc
      121 ggtgatattt atcaaattcg cagccagata tctgactatg cagaccgact atccgggtat
      181 cgcgcgtctg tttttgtttg cgaattcagt caatgcgcat ggtatttata ccccgccaag
      241 acccggtgaa tatcctgcac ggccttctgc tttgtagaag gcttcttcgc tatcataatc
      301 gccagattcc agcgcttctt tagccagcca gatgcgtgcc ccagtgcctg catttggctc
      361 cagttgctgg aggcgttttg catcttctag gagtagagcg ataacgtgtt ttaattctgt
      421 ctcgttcatt ttactcacct gaatgtcttc ccaaccaacg acgtgcgcca gcttcggttt
      481 taaacgtttt gcttttggta tacgttatgg cggtgaatgt gccgtcctgg ttggggaaca
      541 cgccgtacac cggagattcg ttgttgccaa gatcgatagt attcatgttg accccatttc
      601 cccttaacgc cggggtagcg gaactgtttg ctgagaacac cgtgcggtgt cttgatgaat
      661 aaaatttaga ataacctaag gagggtgatc aagattttta tgtagaaaaa cctaagtttt
      721 ttgatgtaaa aaacacaagt gtttgaaagt ttgtgctttt tattacaggg tgtggagaaa
      781 aaaggggatt atttgtttgc gcttcttttg cgagctttga gtagttcttc aaaaagtttg
      841 ttgaaatttt caactcgagc acgcatctct gacaacaggg ccttttgctc tgactcaggc
      901 agtgcgtcga acagttgaag taactctttt tgatcttctg tcagaatggc tggctgatta
      961 tccgggatcg gttcgcctgg ttgtttatct tcatccccaa aaagaagcca agtcggtgag
     1021 cactgaagcg cttggctcag tgcgaataat cttttccccg ccggctgtgt ttcatctctt
     1081 tcccattgag aaattgttac gtgagccact ttgaccagct tacctaatgc ggcctgagac
     1141 agttttaatt ttttacgcct atgtaagagg cgagcaccga aggtttcgtt tttcatatta
     1201 gggaattcta atttttcttg acttaggttt ctctacggcc tggtttcctt aggaaaatct
     1261 aaggagctcg atatgttgaa aattgatgct atagcgtttt ttggcagcaa aacaaagctt
     1321 gccaatgccg caggagttag gttggcaagc attgctgcat ggggggaact ggttcctgaa
     1381 ggtcgcgcga tgcgcctgca agaggcatct ggcggggaac ttcagtacga ccccaaagtt
     1441 tatgacgaat atcgtaaggc aaaacgacct gggaaggtga ttcatgaaaa tcaggcatga
     1501 gcacatcgaa tcagtgctgt tagccttggc agccgaaaaa gggcaggcgt gggtcgctaa
     1561 cgcaattact gaagaatatc tgcggcaggg gggcggcgaa ttgtctctgg taccaggcaa
     1621 ggactggaac aatcagcaga atatctatca ccgttggttg aaaggtgaaa cgaaagcgca
     1681 aagggaaaaa attcagaaac tgatccctgc ggttctggca attcttccgc gtgagctgcg
     1741 tcaccgactc tgtatcttcg ataccctgga acgccgtgca ttactggcgg cgcaggaagc
     1801 gttgagtacg gcaattgatg cgcatgatga tgctgtccag gccgtttacc ggaaagcaca
     1861 tttcagcggt ggtgggtcgc ccggcgattc tgtcgtagtg cattgattga aattaatcg
//
LOCUS       DAEEUE010000242         1913 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.247, whole genome shotgun sequence.
ACCESSION   DAEEUE010000242 DAEEUE010000000
VERSION     DAEEUE010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.247"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(53..1702)
                     /locus_tag="KLK84_004221"
     CDS             complement(53..1702)
                     /locus_tag="KLK84_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HBE2011303.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALTVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          451 a          506 c          492 g          464 t
ORIGIN      
        1 gaagaggata attttacagc cagcaggaag ttagcggctg gctgtaaaaa tattatggtt
       61 cgccgtacaa accaattaaa cggcgtacca caccgttagt ccagccaaac ccatcctgca
      121 acggatactc gccaccgccg ccttcgcggg gaacaccatc ggcaatatgg tatttctcga
      181 tcattttgtg ctgttccaga tagaactgat tcaccgtttt cagccagctg cgcgcgattt
      241 catcacccag aaggtcatcg ccatacattt taaatccctg aattgccatc cattgtaacg
      301 gtgcccagcc gttgggttta tcccactgtt caccggtttc gtactcgctt gccaaaatcc
      361 cgccaggtgt cagtaaccgg ctgcgtacgg cgtttgccag acgatcggcc tgttcatggt
      421 tcgccatgcc gacatagagc ggcacaatgg cggcagcgga aaacagcgcc agttgttcgc
      481 gtcgccagtc gtaatcgcgg tagatgccgt tttcatcatc ccagaggtaa cggtttaccg
      541 catcgcgacg ggcactggcc ttctggcgga acagcgcttc tgtctctttc tcgcctttca
      601 gcgccgagat gttggcgatg gcgctctcca gtttaaacag gaaggcattc agatcgatgg
      661 ggatgaactg ggtggtacga atgctcgcca gacgaccagt atcacgcagc caacgggaag
      721 agtaatccca accggaggcc gctcccgcgc gtaaatcgcg gtacacctcg ttgggcgggc
      781 gaccagaatg tttcgcggtt tcaacgtcct caagccagga ttcgtcacgc ggcgtgtcgc
      841 gatcgtccca gtaacggttg agcagcgatc cgtccggcat ccgcacaaca tggcgatagg
      901 cctgattagg gatcaacgat tctgcaccgt ccatccagaa ggcatattcc attttaaggt
      961 ggtcgagata gcggcgcgca ccgcgtacac catcttcttc aaacaactcc accgtcagcg
     1021 caaaaaccgg tggttgcgag cggctcaaat aataggtgcg gttgccgttg gggatgtgac
     1081 cgtagttttc gatcatccag gcgaagttat cggccatgca tttcagcaaa tcttcccgac
     1141 cactttccgc cagtcccagc atggtgaaat aggaatccca atagtaggtt tcgctaaaac
     1201 ggccgcccgg gacaatatat gactgcggta gcgccagcag agaagaccac ggaatgtgat
     1261 cctgtggttc gcgggttagc accggccaca gctggtcgat atgctctttc agggaatttt
     1321 gcgggtccga tacatactcg ctggagtaga cctccggcag ccagaagtga ttttcaacaa
     1381 acttgcgcaa gtcaaaatca cgatgacggc gcactttacg gtagcggatt aaaatatcca
     1441 gcgggtccat tttgggtgcg cagtcgggaa aggttttact gtcggggaaa attttcgccg
     1501 actgaacatg ctcgaacagt tcgagatagc gatcggcagg cgtcagcgca tcagaggcgg
     1561 gcagcccttc aatcatctcg ggttccggtt ctgcctcgat catctcatcc agttttaatt
     1621 catacgggtc cagttcatag cagagatcga cctcgatcat cagttcgtct ggattagggt
     1681 tttgaatttt ctgattgagc ataaggatcc aacctccgaa attcgtcgct aattatcggg
     1741 cttttcttta agggtagtca ttcgctccgc ctggtgggcg acaaagcgtg cggtggatca
     1801 cgtttattaa cttgtcaaaa aatataacgc actaatatca atggaattta taaaaatacg
     1861 gatttggtgt tgtttttcat tttcagaaca ttcaatcctg ctctcgccgc aat
//
LOCUS       DAEEUE010000243         1850 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.248, whole genome shotgun sequence.
ACCESSION   DAEEUE010000243 DAEEUE010000000
VERSION     DAEEUE010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.248"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..258)
                     /locus_tag="KLK84_004222"
     CDS             complement(<1..258)
                     /locus_tag="KLK84_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011304.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMS"
     gene            283..522
                     /gene="relB"
                     /locus_tag="KLK84_004223"
     CDS             283..522
                     /gene="relB"
                     /locus_tag="KLK84_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HBE2011305.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            522..809
                     /gene="relE"
                     /locus_tag="KLK84_004224"
     CDS             522..809
                     /gene="relE"
                     /locus_tag="KLK84_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HBE2011306.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            881..1036
                     /gene="hokD"
                     /locus_tag="KLK84_004225"
     CDS             881..1036
                     /gene="hokD"
                     /locus_tag="KLK84_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBE2011307.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1254..1469
                     /locus_tag="KLK84_004226"
     CDS             1254..1469
                     /locus_tag="KLK84_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011308.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEI"
     gene            1572..1850
                     /locus_tag="KLK84_004227"
                     /pseudo
     CDS             1572..1850
                     /locus_tag="KLK84_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          507 a          409 c          467 g          467 t
ORIGIN      
        1 tgacatgcta aagcaaaaaa tagatgaata agttgagttg tgcatatgta gcctgaccgt
       61 cacaaagtat atggtgtctg taccagtaag atgatggccg gactctttaa aaaagagctg
      121 acctgcacaa tacaggatgg acttagcaat ggctgctcct ggcacaaagc ggacagtgat
      181 caccgttctt acgactactt tctgacttcc ttcgtgactt gccctaagca tgttgtagtg
      241 cgatacttgt aatgacattt gtaattacaa gaggtgtaag acatgggtag cattaacctg
      301 cgtattgacg atgaacttaa agcgcgttct tacgccgcgc ttgaaaaaat gggtgtaact
      361 ccttctgaag cgcttcgtct catgctcgag tatatcgctg acaatgaacg cttgccgttc
      421 aaacagacac tcctgagtga tgaagatgct gaacttgtgg agatagtgaa agaacggctt
      481 cgtaatccta agccagtacg tgtgacgctg gatgaactct gatggcgtat tttctggatt
      541 ttgacgagcg ggcactaaag gaatggcgaa agctgggctc gacggtacgt gaacagttga
      601 aaaagaagct ggttgaagta cttgagtcac cccggattga agcaaacaag ctccgtggta
      661 tgcctgattg ttacaagatt aagctccggt cttcaggcta tcgccttgta taccaggtta
      721 tagacgagaa agttgtcgtt ttcgtgattt ctgttgggaa aagagaacgc tcggaagtat
      781 atagcgaggc ggtcaaacgc attctctgaa ccaaagcatg acatctctgt ttcgcaccga
      841 aggtgacact tctgctttgc gttgacagga gaagcaggct atgaagcagc aaaaggcgat
      901 gttaatcgcc ctgatcgtca tctgtttaac cgtcatagtg acggcactgg taacgaggaa
      961 agacctctgc gaggtacgaa tccgaaccgg ccagacggag gtcgctgtct tcacagctta
     1021 cgaacctgag gagtaagaga cccggcgagt gagaaatccc tcgccacctc tgatgtggca
     1081 ggcatcctca acgcacccgc acttaacccg cttcggcggg tttttgtttt tattttcaac
     1141 gcatttgaag ttctggatgg tgccggaata gaatcaaaaa tacttaagta gcgcgcaggg
     1201 ataaaaggga tggaccccga acaggggagt gctacttatc tggaaggatt ctgatgatga
     1261 acatcgaaga actgcgtaaa attttttgtg aagatggcct ttatgctgtg tgcgttgaaa
     1321 atggaaatat tgttagtcat taccgcattg tgtgtttgca aaaaaatggg gctgcgttaa
     1381 ttaattttgt ggatgcccga gtgacggacg gatttatctt gcgcgacggt gagtttgtca
     1441 cttcattaca ggcattgaaa gagatctgaa taaaagctgg cttttctgct ttttcagaag
     1501 aataaactca tctacaatct tgcgcggggc tgaactcccg ctgagtaaca ccgtgccacc
     1561 ggagaaaacc gatggcacgc aacgcaaaat attacaatca tgataattcg accgttcttg
     1621 cccacacgca cgagcggtat tctcacgcat ttaagtcaga ctggtaccag catccctcat
     1681 gcactgaaga acaggccgaa tggcttattc agtgttaccg caggcgcgga tgcgaggtta
     1741 aaaaagccct tagcctcgac taccgtcact agataatctc cgtcaggctc ccttactccg
     1801 aacagccacc gcgtccgtcc cgcacattcc agcaacggat ctggaggtaa
//
LOCUS       DAEEUE010000244         1833 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.249, whole genome shotgun sequence.
ACCESSION   DAEEUE010000244 DAEEUE010000000
VERSION     DAEEUE010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.249"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..180)
                     /locus_tag="KLK84_004228"
     CDS             complement(<1..180)
                     /locus_tag="KLK84_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HBE2011309.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQMYGVIV
                     MTTQISVETLSPITHN"
     gene            complement(321..875)
                     /locus_tag="KLK84_004229"
     CDS             complement(321..875)
                     /locus_tag="KLK84_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HBE2011310.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(872..1162)
                     /locus_tag="KLK84_004230"
     CDS             complement(872..1162)
                     /locus_tag="KLK84_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HBE2011311.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1162..1761)
                     /locus_tag="KLK84_004231"
     CDS             complement(1162..1761)
                     /locus_tag="KLK84_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HBE2011312.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          445 a          497 c          421 g          470 t
ORIGIN      
        1 gttatgggtg atcggggaga gagtttcaac agagatttga gtggtcataa cgataactcc
       61 gtacatttgg aaattatcgc caccgacgac gccaatcgaa ctggtggcga actgtgcagg
      121 gttggcgtaa ccgggtacgg aaaccggcga gcctttcggc tcccccacac agcccgccat
      181 aaatcgcgaa tgtgactgtg caaacgatat gaaaaaagac gcgggcgcgt ctcatatcgc
      241 tccgtaaaca tccgggacgc caatcccgac gccagatttt gctggcgcgt gaggaatata
      301 gccccggata acagattgag tcaacaaacg gtttttagat ccccggaaga gaatgcatca
      361 cgcatcggta gatagagcat atattcagca agatttagcc atacatctac ccgactgcag
      421 cacgtccgga aacaccactc agggtgtgcg tcattcagca attcagccat tttacgctta
      481 gtcatccccc gcccttcata ccgttgccgg aggacactaa tcaatccagg atgctctgcc
      541 agcacctcac ttatgactct atcaatacat aacgcctctg catcagtaca atgcgccagc
      601 caggtctttt gcttgccatt gatcatctct cgcaaaaacg cttccagctc aggtttctct
      661 attcccgctt ttttcatcct gcgcagggct tcattgacgg ctgttttcgt caattttttg
      721 gatgccagca actggttaaa catatttccc gtcttaccgc cgccaatata cgaccagcgc
      781 ccccacatgc gcagttttcc ctgaatccag acactttcca gcgtggtgag acgaaggtgt
      841 tccccgcttt tgcctgtatt tgttgggtaa atcataaata accttccttt ctccagattt
      901 cttgtgtgcg aaaaacacct tctgcatgca tcaggcgtaa ttcttctttg gtgtaatcgc
      961 tggtttttac ccgcccgtcg attaaatcgt ggcatgagct acaggcaatc gctgcctgca
     1021 tatcgtgtgg ttttgtcgct gttccgcacg tccccgccag cctgtaatgc gccagcacag
     1081 aagtttcggg attgtgattg cagtagccag gaattctgac ggtgcacatc tgcccccgcg
     1141 ccgctttacg taaatccacc attacgcaaa ctccagtaac tgcgcggcca cattttcgac
     1201 ttcctccgga gaggaaaatt tacggaacag gatccagttc cacagcacat tcagtacaga
     1261 tttataaacc tgctgaaact cggtttcgtc catgttcgca aatgcgatag attttgccct
     1321 gcgcccacga ctaccatcag gataaatatg ctcggtgtaa aatccggcct gaatggttac
     1381 ccactcgcgg aaagcgtcaa acgactttag caatgccgta tcccgggttc tgcatgtcgc
     1441 aactgtatta aggtattgct ctgcggcatc actcagggct ggagtgtgtt cccgacctac
     1501 tgattcgcac aggtaatcaa cgaagcctga taccagtttt cgttcgcgag gcgtgatcgc
     1561 cccaccgacc ggagtccagt aatcaaatcc cagttgcagg agtttgaaaa aacgcttgtg
     1621 gaatgcgtag ttacgcacac gcttaaagtc tgcgtgtatc cactcaccta ttttgatttg
     1681 atgcaaaaaa tcgcaactct ccggcgtcgc cgggagaagt aatccggaag aggtttgttt
     1741 gaccagttgt atatgcgcca tcgtagttct ccgctggcgc agtagaatgg gtgttcagcc
     1801 cgttatgtag tataccagaa ttaatgccaa tac
//
LOCUS       DAEEUE010000245         1809 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.250, whole genome shotgun sequence.
ACCESSION   DAEEUE010000245 DAEEUE010000000
VERSION     DAEEUE010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.250"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            173..1198
                     /locus_tag="KLK84_004232"
     CDS             173..1198
                     /locus_tag="KLK84_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBE2011313.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1809
                     /locus_tag="KLK84_004233"
                     /pseudo
     CDS             1266..1809
                     /locus_tag="KLK84_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          421 a          420 c          462 g          506 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctagct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtggg
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgtc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cgcagctggg agacggattt
     1801 attgcgttt
//
LOCUS       DAEEUE010000246         1784 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.251, whole genome shotgun sequence.
ACCESSION   DAEEUE010000246 DAEEUE010000000
VERSION     DAEEUE010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.251"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            54..1784
                     /locus_tag="KLK84_004234"
                     /pseudo
     CDS             54..1784
                     /locus_tag="KLK84_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
BASE COUNT          468 a          402 c          445 g          469 t
ORIGIN      
        1 tcagtgaccc gataaaaatg acgctgtaag caagggataa ggataggcgt attatgatga
       61 cgaccagaat agtggttggc ctcacggcag ggacgtgtct gattttctcg caaaacctga
      121 tggccgaggt cagtgtattc aatccggcgc ttctggaaat caaccatcaa tccggagtcg
      181 atattcgcca gtttaatcgg gcaaacctga tgcccccagg tgtttatagc gttgatattt
      241 ttatcaacgg taaaatgttt gaacgtcagg atgtgacatt tgttcaggat aatccagatg
      301 ctgatctgca cgcttgcttt attgccatta aaaaaacact gtcctccttt ggcataaaag
      361 ttgatgcgct caaatcgttc aatgatgtgg atgagacggt ttgcctcgat cctgctccac
      421 gtattgaagg ctcatcctgg cagtttgaca gtgataaatt gcagctgaat atatccattc
      481 accaaatcta catggacgcg atggcttatg attacatcag ccccacgcgt tgggatgagg
      541 ggattaatgc gctcaccatc aactacgatt tttctggttc acatacacta cgttcagatt
      601 atggttcaca agagacagat accagttatc tcaatctgcg caatggactg aatattggac
      661 cgtggcggct acgtaattac agtactttaa acaccagcga tggccgtgcg gaatacaact
      721 ccattagtac ctggatacag cgcgatattg ccgcgttaag aagccagatt atgattggtg
      781 atacgtggac ggcgagcgat attttcgaca gtacgcaaat tcgcggcgcg cgtttgtata
      841 ctgataacga tatgctaccc gccagccaga atggctttgc tcctgtggtt cgtgggattg
      901 caaagtccaa cgccaccgtc atcattcggc agaatggcta cgtgatttat cagtcagccg
      961 ttccacaagg tgcttttgag atcaccgatc tcaacaccgc aagtacaggt ggcgatttgg
     1021 acgtaaccat caaagaagaa gacggtagcg aacaacgatt cacccaacct tatgcttcat
     1081 tggcgattct taaacgtgaa ggtctgacag atgttgatgt cagcgtgggt gaattgcgcg
     1141 atgaagacgg atttacaccg gacgtccttc aggcgcaaat acttcatggt ttttcccacg
     1201 ggatcacttt atatggaggt atgcaggctg ctgaaaatta tggttctgca gctctgggtg
     1261 tcggtaaaga tcttggcgct ttgggcgcaa tttctttcga tgtgacacat gctcgtgcga
     1321 attttagcca tgatgataca gaaacgggtc agtcatatcg ctttctctat tcaaaactat
     1381 ttgacgacac agacactagc ttgcgcctgg ttggctatcg ttactccacc gagggctact
     1441 ataccactca atgagtgggc atcgcggcgc aacagccctg aagacttttg ggaaacaggt
     1501 aaccgacgta gtcgcgtgga gggaacgcta acgcagtcgt tggggagaga ttatggcaat
     1561 ttatacctga cattaagccg gcaacaatac tggcataccg atgatgtcga acgattaatg
     1621 caatttggct acagcagtag ctggaagcgt ctctcgtgga acgtctcctg gagttattcc
     1681 aatactgcca gacaggggac ggggaacaac catgccagtg ataacaccag tgagcagatc
     1741 tacatgctct ctttatctgt tcctttatcg ggctggtggg gtaa
//
LOCUS       DAEEUE010000247         1753 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.252, whole genome shotgun sequence.
ACCESSION   DAEEUE010000247 DAEEUE010000000
VERSION     DAEEUE010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.252"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(485..631)
                     /locus_tag="KLK84_004235"
                     /pseudo
     CDS             complement(485..631)
                     /locus_tag="KLK84_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            685..867
                     /locus_tag="KLK84_004236"
     CDS             685..867
                     /locus_tag="KLK84_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HBE2011314.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(968..1297)
                     /locus_tag="KLK84_004237"
     CDS             complement(968..1297)
                     /locus_tag="KLK84_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HBE2011315.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1469..>1753)
                     /locus_tag="KLK84_004238"
     CDS             complement(1469..>1753)
                     /locus_tag="KLK84_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011316.1"
                     /translation="HFVLLNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLN
                     DAVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          483 a          406 c          413 g          451 t
ORIGIN      
        1 actggtataa caatcgacga ttgctggaaa ggctgggcca catccctccg gcagaagcag
       61 aaaaagctta ttatgcttcc atcggaaacg atgatctggc agcctgagtt cacagataaa
      121 acactctcca ggaaacccgg ggcggttcaa atagcaatgt ctgtcgcaca acaaccacaa
      181 tgtcacaaat aaaaaatgag aataaccagt tccctgaagg cgacgtagcc cggtacaaga
      241 gtaacccacg ttagcgcttg gcgacggcgg aacaaagctg attcgcgacg taagaaagtg
      301 gcaggctcat atgacggacc tctgtaatgc ggtatccaac ccgcgaatat cagcatgatt
      361 caccgtcgga tttactgcta cgtcgccctc agggaattaa acctaaaaag aaaatcgtca
      421 aaattagaga ttccggtttg acacggaaag tcatatcagc gtcaacggag caccgtattg
      481 acgcttattt attggtgaga actacgttcc atggcaggag ttcgtcaaca cggttggagg
      541 gccattcagg cagtatgctc agaatatggc gcagatacgc ttccggatcg ataccgttca
      601 gacggcaggt gcctgctttt ccggcaacaa gcaggaatct cagcgctgaa gcaggaatta
      661 gaggtaaaaa cgccctaccg ggccatgaat cacccggtca ttggagtagt aaccaaagca
      721 gatttagcca gcatggaaca aatatctttg gtgaaaagtt ggttacggga agctggagcg
      781 cacaacgtgt tagtaaccag tgcagttaat aacaatggag ttacagaact ttttgccttg
      841 ctgcatacag aagaaggctg ttgttaattt cgattggttc tcagatgaga atgatgacgg
      901 tgatacacag ataagaatca atgagcaatg agggttgccg ggcaaccctc attgaataaa
      961 acgggaatta cttcgcttcg ccgtttttca tttcgcccat agctttcagc tttttggaga
     1021 tatcgcggcg ttctttggag agctcggcat ttttgatgat gtaatcgtca acgcgatctt
     1081 catagtcacc tttcatgctg gcgatgatgc cctggattgc ttcaacgctc atccctggct
     1141 tgatgtaatc gctcaggttg tccagcaaca ggacgcgctt ctggttgtca cggatctttt
     1201 tctcaacgtc ctgaatttca cgttgcagtt tgttcttacg acggaacaga cgaacaaatt
     1261 ccagtacgtc ctggaatgaa ggcttggtag tttccatttt tatacccctg ataatgtgag
     1321 agtcggattc gtttaagcaa ccgctattcg ttagggccaa cattactcat agctgccgca
     1381 gatgacaatg cttttatccc ttttcactat catacccttt atccttgctg aatcgaagca
     1441 gcagcaagat gattctgaag ttcagaaatt atttgcgcaa ggcccggcaa atcagattcg
     1501 atagcaactc aagctgatgt gccgtttcca tgttcagcca cacataacca tagattggtg
     1561 tttccacgac tttcaggtca tggtggttat cgagcaactg acgcagctct tccacagcat
     1621 cgttcaattt ttccgtattg gcaaaaaccg gctgcggatt accttcgtac agcgcatgaa
     1681 caaggctcag cagtatttgc tgcatcatgt gctgggtatc acaaagtttt tgcgcgttca
     1741 ataacacgaa atg
//
LOCUS       DAEEUE010000248         1725 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.253, whole genome shotgun sequence.
ACCESSION   DAEEUE010000248 DAEEUE010000000
VERSION     DAEEUE010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.253"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..243
                     /locus_tag="KLK84_004239"
     CDS             <1..243
                     /locus_tag="KLK84_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HBE2011317.1"
                     /translation="AENDALRRKLDNGGRVLVKGKCPVPSSAETSSASGMGNDATVEL
                     SPVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(332..844)
                     /locus_tag="KLK84_004240"
     CDS             complement(332..844)
                     /locus_tag="KLK84_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HBE2011318.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1039..>1725
                     /locus_tag="KLK84_004241"
     CDS             1039..>1725
                     /locus_tag="KLK84_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011319.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGI"
BASE COUNT          506 a          386 c          437 g          396 t
ORIGIN      
        1 gctgaaaatg atgctcttcg gcgcaagctt gataatggtg gtcgggtgct cgtcaaagga
       61 aaatgccctg tgccatcctc agccgaaacc tccagcgcct ccggcatggg caatgatgcc
      121 accgtcgaac tctctccagt tgctggacga aacgttctcg gtatccggga cggaatcatc
      181 agcgaccaga cagcattgag aatgcttcag gagtacatca ggatccaatg ccttgggggg
      241 tagcggtaat tttactcatt atccttcaaa tcagattctg ttgtcagagg aatgggggaa
      301 gctggattcg caagagcttt ttatgggcag tttagctaaa aaaaggcaac acgaagctaa
      361 aactaccaac ataaagtgct gaccagctag cagctgaaat cagactcact gtatacactt
      421 tcactggatg aagttgcaac attcctgcca ccaaaggagc aatgtaacgc agcacggcga
      481 taaagcgcga agtgaaaagt attgacacag agttattttg caactgaagg cgaacccgtt
      541 ctattgtatt ggaacggctc gatattatac atgcaagttg agggatatgc ccaattatta
      601 taccaagatg gtaattaacg atagtaccgc accatgcgcc actcatgacc gtgattccag
      661 cttcccatgg tgaaagagtc gtcttgctga ctgtaattac agtggccatc ataattgatg
      721 ctggtggcaa tatcgccgaa atgagtaaag tggattttgt gaacgctatt ataaaaagaa
      781 gcccccatag gcgatatgga tgaagtacaa aatagttcat caaagcattt atccactcca
      841 tcaatgtctt tccttctgtg aatttttctt tgctatttaa ccgtgatacg acttaattta
      901 ggcttaaaca ccttacattt acaaggtact cctgtggggg gccctgccac ggagcgtcgc
      961 aggagtgagt gatgaggaag aattaaagca acctattgtg acaggcagcg ataacagtaa
     1021 atcacaggaa gcataatcat ggcaaaaccg gactgggagg ccatcgaatc ggcataccgg
     1081 gccggagtcc ttagcctccg tgatataggc gagaaatacg gcgttacaga aggggctatc
     1141 aggaagaggg ccaaaaagct tgattgggca cgcagtggcg gtacgcaggt ttgcaaaaat
     1201 ggtacgcaaa aaaggaaagt gcgtaccagc agaaagcctg ccattactgg ccttacacaa
     1261 aaaagtacgc aactaaaaac agaatctaca ccggatacga aaccgatacg cggaatgcgt
     1321 accgatcccc cgactaaccc attccaaccc ggtaaccagc aggcattaaa acacggtggt
     1381 tatgcccgtc gccttctgct caaagatgag gtgatagagg acgctaaagc gttgacgctc
     1441 gaggacgaat tatttcgcct tcgtgctaac aaccttgtcg ctgcagagaa tattggtcgg
     1501 tggttggtgt cgctggaaga tgctaatggg gaccaggaaa ggaagatgct gattgaaaat
     1561 atcagcgccg ccgagaaagc aatgatgcgc aatacagttc gtattgagtc catcgttggc
     1621 acgcttgcga cggtaggaaa aatatttgct gatacagcct accgcaaggc cgccactgat
     1681 aaggtgtctc tggaggctga tcgtcttcgc cgtgatgcag gtatt
//
LOCUS       DAEEUE010000249         1706 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.254, whole genome shotgun sequence.
ACCESSION   DAEEUE010000249 DAEEUE010000000
VERSION     DAEEUE010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.254"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..87)
                     /locus_tag="KLK84_004242"
     CDS             complement(<1..87)
                     /locus_tag="KLK84_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HBE2011320.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQWLD"
     gene            complement(768..842)
                     /locus_tag="KLK84_004243"
     tRNA            complement(768..842)
                     /locus_tag="KLK84_004243"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(807..809),aa:Gly,seq:tcc)
     gene            complement(846..920)
                     /locus_tag="KLK84_004244"
     tRNA            complement(846..920)
                     /locus_tag="KLK84_004244"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(885..887),aa:Thr,seq:tgt)
     gene            complement(923..999)
                     /locus_tag="KLK84_004245"
     tRNA            complement(923..999)
                     /locus_tag="KLK84_004245"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(963..965),aa:Arg,seq:tct)
     gene            complement(1001..1077)
                     /locus_tag="KLK84_004246"
     tRNA            complement(1001..1077)
                     /locus_tag="KLK84_004246"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1041..1043),aa:Ile,seq:cat)
     gene            complement(1267..>1706)
                     /locus_tag="KLK84_004247"
     CDS             complement(1267..>1706)
                     /locus_tag="KLK84_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HBE2011321.1"
                     /translation="GKTGNMFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRD
                     MINGKQKSWLAHCTDAEALCIDRVISEVLAEHPGLISVLRQRYEGRGMTKCKMAELLN
                     DAHPKWSLRTCERRIEHWLKVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          468 a          451 c          348 g          439 t
ORIGIN      
        1 atccaaccac tgcaaaaacc agtagccggc actgccggcg gaggtgccgt aggcaatgcc
       61 tgttgatatt ttttccatct gatacatatc ccgccccccc gacagacctg tgctatcgga
      121 aagaaaaaag gccatcagca aaactctgat ggcctgaatc acctttacca atattgtatg
      181 aaaaaacacg cacgactaat tgacaataat tttcatttcc attaaaaaag atcgcgtagc
      241 attcttaatt catgaggaac tttacccgcc agcaatctga gtagcgaaag ctgttcatcc
      301 ccaatgtttt tgctggcggg tccttttttc tacggtcctc ctccccggag ggggcatttc
      361 attatttttc taatcgttac tggtgagaag gcggaagaaa aagccagttc ttcgccccta
      421 aataacagca tgattccagt ccatgcaaaa tctgatttcc agctcacaca taccgagcac
      481 tgtacaaaaa accgccaacc aaggcagtta agagtgtgtt cccggggttt gcttagaata
      541 ttttgtaagt tgtccgaaga gatatttaca acaccagaat gatgcattat cagtcccctg
      601 ccagaggttc actacgtaac ctgaccgaca atgcaccatt ctggtgctga aaaaagagca
      661 ctggaactgc aacaaaacaa catcacagaa caggagatgg taaggagtca gacattccca
      721 cacaatattg tgtcagtgca aataacaacc ttcgtctcaa atctcgctgg agcgggcagc
      781 gggaatcgaa cccgcatcat cagcttggaa ggctgaggta atagccatta tacgatgccc
      841 gcatatggtg cgactaccgg aatcgaactg gtgacctact gattacaagt cagttgccct
      901 gcctgctgag ctaagtcggc gctggcccac caccgaggac tcgaacctcg caccgtcaac
      961 ttagaaggtt gatgctctat ccggatgagc tagtggtggt tggtggccct tgctggactt
     1021 gaaccagcga cctggcgatt atgagtcgct cgctctgacc aactgagcta aagggccgga
     1081 ggcagaataa taaccatatg tcatcacatc tgcaaactca tctgatcacc agcacgttta
     1141 acgtcctgtg ccgtttttca ggtataaaaa aaaccgcata aagcgggctc tttcaaatgt
     1201 ccatgtctgc tattcgcctc gcggtacagc tttgcgaagc gtacaagaat tgaagcagtt
     1261 tttacgtcaa aaagcaataa cttttttctc tataccaaaa gccataacca ttggtttgta
     1321 caaaataaat tctgccacct ttagccaatg ctcaatgcgt ctttcacagg ttcttaaact
     1381 ccatttcggg tgtgcatcat tcagcagttc agccattttg catttggtca tcccccgccc
     1441 ctcataccgt tgccgaagga cgctaatcaa tcctggatgc tctgccagca cctcacttat
     1501 gacccgatca atacataacg cctctgcatc agtacaatgc gccagccagc tcttttgctt
     1561 accgttaatc atatcccgca aaaaagcctc aagttcagac ttgttcagac ctgctttttt
     1621 catcctccgg agcgcctcgt taattgccgt ttttgtcagc tttttagagg tcaacatcag
     1681 gttaaacata ttccccgtct taccgc
//
LOCUS       DAEEUE010000250         1600 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.255, whole genome shotgun sequence.
ACCESSION   DAEEUE010000250 DAEEUE010000000
VERSION     DAEEUE010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.255"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            154..477
                     /locus_tag="KLK84_004248"
     CDS             154..477
                     /locus_tag="KLK84_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBE2011322.1"
                     /translation="MLLKEEEIKSHLRLDDGLYSDGDFLKLLAQAVQKKTETYLNRKL
                     YAPEETIPEDDPDGMHLTDDVRLAMLMLVSHFYENRSTITDVEKLETPMSFRWLAGPY
                     RIVPL"
     gene            474..890
                     /locus_tag="KLK84_004249"
     CDS             474..890
                     /locus_tag="KLK84_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBE2011323.1"
                     /translation="MKIRQSQTSATYLLPDPGELNRRIKIRLRVDEPTADFGTEPTYP
                     ESFDVWAKVAQPGAAAYQGSVQTENIVTHYFTIRFRHDITADHEVVYYGQEYRIRRIR
                     DLNGQRRFLLLECEELRTARRRGECHESDSIFTRRL"
     gene            856..1380
                     /locus_tag="KLK84_004250"
     CDS             856..1380
                     /locus_tag="KLK84_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011324.1"
                     /translation="MNQTAFLHVDFKQPEELEFNRARLRRAFVQIGRVYMRDARRLVI
                     KRGRSGPGENPGYQTGRLARSIGYYVPKKTTRRPGLMVKISPDQKNGQGNRRFPEGAP
                     YYPAFLYYGVRHSAYGMDKKDKRQKKHHSSTFRLAPRNNFMADVIERRRHWTQELLSR
                     ELQRSLRPVKRKHK"
     gene            1377..>1600
                     /locus_tag="KLK84_004251"
     CDS             1377..>1600
                     /locus_tag="KLK84_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011325.1"
                     /translation="MKLTTIIAALRERCPRFEERVGGAAQFKAIPDAGKLRLPAAYVV
                     PSDDAPGEQKSQTDYWQDLTEGFSVIVVLS"
BASE COUNT          391 a          379 c          456 g          374 t
ORIGIN      
        1 gttcgtgggt ttttatacca ccaagcgaac cggcggtatg ctggtggatt ctcaggcgat
       61 taagctgatg aaaattgggg ctgcaacccg ccagaaagcc gctgcgtaat gcggtttttt
      121 gtgcccgcgt aatcgcgggc acaggaggaa aatatgctcc tgaaagaaga ggagattaaa
      181 tctcatctcc ggctcgatga tggtttgtac agtgacggcg attttctgaa actgctggca
      241 caggcggtac agaaaaaaac agagacatac ctgaacagga agttgtatgc accggaagag
      301 acgattccgg aagatgatcc tgacgggatg catctgactg atgacgttcg tctggcaatg
      361 ctgatgctgg tcagtcattt ttatgaaaat cgctcaacga ttaccgatgt ggagaaactg
      421 gaaacgccaa tgagtttcag atggcttgct ggcccttaca ggattgtccc gctatgaaaa
      481 tcaggcaaag tcagaccagt gccacttacc ttttaccgga tcccggagag ctgaatcgcc
      541 ggataaagat ccgccttcgt gtggatgaac ccaccgctga ttttggtacg gagccaacgt
      601 atccggagtc gtttgatgtc tgggcaaagg tggctcagcc aggcgctgcc gcttatcagg
      661 gctcagtgca gacagaaaat atcgttacgc attattttac gatccgtttc cggcacgaca
      721 tcacggcaga tcacgaagtg gtttattacg gccaagagta ccggatccgg cgaatacgtg
      781 acctgaacgg tcagcggcgt tttttattac tggaatgtga agaactgcgt acagcgcgac
      841 gacggggtga atgccatgaa tcagacagca tttttacacg tcgactttaa acaaccagag
      901 gagctggagt ttaatcgcgc ccgtctgcgt cgggcgtttg tgcaaatcgg gcgcgtttac
      961 atgcgtgatg ccagacgtct ggtgattaaa cgcggacgct ccggtccggg agagaacccg
     1021 ggatatcaga ccgggcgtct ggcccgctcc attggttatt acgttcccaa aaaaacgacg
     1081 cgtcgccccg gactgatggt gaaaatttcc cctgaccaga aaaacgggca ggggaatcgc
     1141 cgttttcctg aaggtgctcc ttattatccg gcgtttctgt attacggcgt tcgccattca
     1201 gcgtatggaa tggataaaaa ggataagcgg cagaagaagc accattcttc gactttccgg
     1261 ctggccccgc gtaataactt tatggctgac gttattgagc gtcggcgtca ctggacgcag
     1321 gaactgttgt cccgtgagct gcagcgttcg ttacgtccgg taaaaaggaa gcataaatga
     1381 aactgacaac gataattgcg gcactgcgtg agcgatgccc gcgttttgag gagcgtgttg
     1441 gtggcgcggc gcagtttaag gcgatccctg atgccggaaa acttcgcctg cctgctgcct
     1501 atgtggttcc ctctgacgat gcgccggggg aacaaaaatc acagaccgat tactggcagg
     1561 atttgactga aggcttctcc gtgattgtgg tgctcagcaa
//
LOCUS       DAEEUE010000251         1538 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.257, whole genome shotgun sequence.
ACCESSION   DAEEUE010000251 DAEEUE010000000
VERSION     DAEEUE010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.257"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(910..1386)
                     /locus_tag="KLK84_004252"
     CDS             complement(910..1386)
                     /locus_tag="KLK84_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE2011326.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          346 a          410 c          386 g          396 t
ORIGIN      
        1 aaaaaaacaa cagcttacaa atcggcgtaa aagatctatt ctaggcaagg gcgttgcgcg
       61 caacgcaacg cccttttggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctctgcaag cctcgtcagc cgtgattttg cccgatttcg
      181 cgctctccac gctcgatcat gtttttgccg tccgttcacc gttaaaacgc ctcagaaatc
      241 gggacaggat gtgggaatgt cggaccgcgc acgcgcactc agacattccc cacttggtca
      301 gggcttcacc ccgacacccc gcatccgtgc gtaagtgctc aatgtaactt ccggttctct
      361 tccgcttcct cgtcgattcg ctcgctacgc tcggtcatcg acttgcggcg agcgggatca
      421 cctctcgcat gttgtcggtg atcccgttgt tgttctttcg gggatgagtc gtcagatagc
      481 ccttcaaaca cgctcagaag ctctcagacg cacgaaacgg gtttaaatgc ccgtcagggc
      541 atggaaggcg cgaaaaacgc ttacagaggc tctcaggagg tcaggcgcgc cgatacccct
      601 aaccaggggc tttcaggtcg ccaggaaccg cgtcagctcc ctgcaaagcc tatttgcacg
      661 gcacaggcag ctattcggct ggttggtggt cgtagaccgt cggtgttaca gcgatggggg
      721 tctggggttc tccccagacg gtttccggtt gcgctcaagg cgcagccgga accgtggcgg
      781 cgcggtcgtt ctggggatcg gggagcaaaa acacgcaagg gattttgagg cggcaaaaga
      841 gcaatgcagg atagacaatc atggtcaaaa cgttaacacg tagcccctga ttgtccgcct
      901 gccgctggct caatttgaaa acatggccat gattcggcgc tgaatatcac cgtctttgtt
      961 gaccagtgtt ttagtgacaa acgctgcatg gtttttcgta gtgttgtatc ccttccagac
     1021 ttcatgtgga tcgagggcat acgcacaaac cttaccagca acggggattc taactaatgc
     1081 attttttgat tctaagtaat tgagccaccg aataattgtg cttctgctaa ctccaagttt
     1141 ttcagctagg aattgctgat ccgcaacgac agcgccacac gtaggatcga tatgctctgc
     1201 taaaaatgag tacagcttca gtgctgatat gccttggcta tccttcagca gctctcgaac
     1261 gcgttcccaa ccttttgggg atacctgtgt aaaccttgaa ttcttctttg cttcttcgag
     1321 atcttcggtg tttttctttg cctcatctga aagttgtttc agtcgttttt ctgaatcaat
     1381 aaacatgatt ttttccctct gaaaatccca tgcactaatg ttggttcaaa tctaagccat
     1441 catgacacat ctgtgcaaca ccttgttgcg tgttgtcgca tcctggatgc gacaacgttg
     1501 tgtcaccctg gatgcgacag gtaaacaaaa aacacact
//
LOCUS       DAEEUE010000252         1496 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.258, whole genome shotgun sequence.
ACCESSION   DAEEUE010000252 DAEEUE010000000
VERSION     DAEEUE010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.258"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(56..601)
                     /locus_tag="KLK84_004253"
                     /pseudo
     CDS             complement(56..601)
                     /locus_tag="KLK84_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1256..>1496)
                     /locus_tag="KLK84_004254"
     CDS             complement(1256..>1496)
                     /locus_tag="KLK84_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011327.1"
                     /translation="GVYLLPKRFASISFRSTKDAKTVDVKVTRNSNTAGYDLELISVV
                     DVLATGSVKVKAGEFDVEEDASFPLVHVIRFTAAE"
BASE COUNT          436 a          318 c          314 g          428 t
ORIGIN      
        1 tttgaaaacc agaacctcgc ttaggcctgt gtccatatta cgtgggtagg atcagaattc
       61 catccagtcg ttttgggttg ctaatgatgt cctgcatccg cttaatctgg tcgggcgtag
      121 acatataacc ccacagggtg agcagaacct tttttgcggc tacactattt ccttggtctg
      181 atactaatgc catatagccc cataaactat ccctcagcca gacagcatca tagtgggttt
      241 ctgccgctgt tttgtcatct tcgaaagtag aagcaagaat aaacgttccc cagggtttct
      301 ccgtcaacgt taactttcgt gtgttttctg ttaatgttag caatccttga gtttcgtctt
      361 tggtatagtg actctttatt tcatttttta atttggctga gtgaatcaga agacgataat
      421 taggatactt atctggattt aacgataaat aacttttatc tgttgctgcc cccctggaat
      481 taagaatgtt aaatacagat tcgcttgtca cttctgcctc tgtcgcctgg catgtgccgc
      541 acgaaaatat accagcgagc agaagagtta atgcagattt ccctttcata aaataatcca
      601 tcaatcgttt gttaaaagcg tagataacca cttattcaac aacttgaaaa taaggtattt
      661 tacctgggtt gttacaaaag gattgcattg cgtaaacgct ttttatttac aaaaaaatgg
      721 ggaagtgtta cagcgattgc gaaaagcctg aagtgattga attaagataa tatagaaaat
      781 cagatacagc aaaaatggca gattgatcag gtgaagataa attggagcgg gcgaagggaa
      841 cctaggtatc gagggtatcg aagataatca gccgctaacc atttgtttcc ttaaaggatg
      901 atgaatcctt gtggttacag ttatggttac acattaattt atctctggtc aagtcccgaa
      961 gtcctaactt taagacagtg gccccaatcg tccagacttg aaaggggcct atgggggaag
     1021 tcgctgacct ttatctatta aatacctaca gagatttttg tgagattttt taatccaagg
     1081 tcataaccta ctgcccccac aggccgcaaa gtgaagacct gtaagcacca gagcaatagc
     1141 gagtatctat cactccctgt agagtatctg gtagaccaag aggacacaga ggacgaccat
     1201 atacaactcc tgtaagctca tggatgtact ccttatgttc actcagggtt tatagttact
     1261 ctgcggcagt aaaacgaata acatggacca acgggaaaga ggcgtcttcc tcaacgtcaa
     1321 actcacctgc tttcacctta acggaaccag tagccaacac atcgaccaca ctgattaact
     1381 caaggtcata cccagcagtg ttgctatttc gggtcacttt gacatctacc gtcttagcgt
     1441 ctttagttga cctgaaagag atagaagcga accgcttagg caggagatag acacct
//
LOCUS       DAEEUE010000253         1494 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.259, whole genome shotgun sequence.
ACCESSION   DAEEUE010000253 DAEEUE010000000
VERSION     DAEEUE010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.259"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            67..850
                     /locus_tag="KLK84_004255"
                     /pseudo
     CDS             67..850
                     /locus_tag="KLK84_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            857..1494
                     /locus_tag="KLK84_004256"
                     /pseudo
     CDS             857..1494
                     /locus_tag="KLK84_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          414 a          337 c          427 g          316 t
ORIGIN      
        1 cgtgccggat tgttttgttc ggtatcagtt aaatgtaacg ctgcgagcgt tacaaggtga
       61 aaacaaatgg cttcaaactg gataaagctc gaggttatta cgccggataa gccggaaata
      121 ttcaggcttg ctgagattct gaatattgat ccagatgccg cattagggaa ggttattcgc
      181 ttctgggcat gggcggatca acaaatgata gacggtaatg cagattgtaa cgctcgcggc
      241 gttacaaaaa gtgcaataga tcgcatcact tttatgtctg gttttgctga tgcgttaatt
      301 caggttggat ggctggtcga aaatgacggt gggctttctc tacctaactt tgaacgtcat
      361 aacggaaaaa gctctaaaaa acgggcggtt acaaacgagc gagtaacaaa aatacgcgaa
      421 ctgaaacgaa aaggtaacgc tgccagcgtt acacaaacgg atcaaaaagc gttaccagtg
      481 gaagaggaag aggaagagga agatctaaat actgatctcc ccctaaatcc ccctcgccaa
      541 aaacgagcgt ctaaaaaatt cgagccggag gctattgagc tgcccgattg gttgccggaa
      601 acactctggc atgagtgggt ccggttcaga caggcattgc gaaaaccgat tcgaacggag
      661 cagggcgata cgggaactgg aaaaattccg tcagcagggt tttacacctg agcaggtgat
      721 tcgacacagc atcgccaatg aataccaggg cctgttcgcg ccgaaaggtg ttcggcctga
      781 gacgttgctc cgacaggtta acaccgtctc gttgccggac agtgcgatcc cgccaggctt
      841 cagggggtaa cggaccatga aaaatattgc gacaggcggc gttctggaac gcatccgccg
      901 actgaccccg caacatgtaa ccgccccatt cagaacggta gcggagtggc gcgagtggca
      961 acttgctgaa ggccagaaac gtagcgagga gatcaaccgc ctgaatcgcc agttgcgggt
     1021 ggaaaaaatt ctgaatcgct caggcatcca gccgttgcac cgtaaatgct cgtttgcgaa
     1081 ttaccaggtg cagaacgacg gccagcgata cgcgttaagc caggcgaaat ccatcgccga
     1141 tgaactgatg accgggtgta caaattttgc gttcagcgga aaacctgata ccgggtagaa
     1201 tcacttagcg gcagctatcg ggaatcgcct gctgaaagac ggtcagacag tgattgtggt
     1261 taccgtggct gatgttatga gcgccctgca cgccagctat gacgatgggc agtcaggcga
     1321 aaaatttttg cgggagctgt gcgaagtgga tctgctggtt cttgatgaaa ttggcattca
     1381 gcgcgagacg aaaaacgagc aggtggtgct gcaccagatt gttgatcgcc ggacagcgtc
     1441 gatgcgcagc gtggggatgc tgacaaacct gaactatgag gtgatgaaaa catt
//
LOCUS       DAEEUE010000254         1450 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.260, whole genome shotgun sequence.
ACCESSION   DAEEUE010000254 DAEEUE010000000
VERSION     DAEEUE010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.260"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..656
                     /gene="ipaH9.8"
                     /locus_tag="KLK84_004257"
     CDS             <1..656
                     /gene="ipaH9.8"
                     /locus_tag="KLK84_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HBE2011328.1"
                     /translation="VAADATESCEDRVALTWNNLRKTLLVHQASEGLFDNDTGALLSL
                     GREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTMLAEKLQLSTAVKEMRFYGVSGV
                     TANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRTEADRWAQAEEQKYEMLENEYP
                     QRVADRLKASGLSDDADAEREAGAQVMRETEQQIYRQLTDEVLALRLSENGSQLHHS"
     gene            complement(657..836)
                     /locus_tag="KLK84_004258"
     CDS             complement(657..836)
                     /locus_tag="KLK84_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011329.1"
                     /translation="MNKPQLAESQKTALLTEAESVIRPPGRAVRLNRETDESGEARGR
                     ASVFPESVRSVVYAM"
     gene            complement(836..>1450)
                     /locus_tag="KLK84_004259"
     CDS             complement(836..>1450)
                     /locus_tag="KLK84_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HBE2011330.1"
                     /translation="SLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFDDRD
                     QAVPYTATADDVAPTGQRIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAWRTE
                     QEAKPFTFEWNGRIWNAGPNSLGRLYPVVMAAKSDIVRDVMTWGDADNQQVKLSMPEL
                     EELAAAMVQAQVDRNDEIYRRQREMKEELSGLDDLASIRAFDVE"
BASE COUNT          328 a          416 c          376 g          330 t
ORIGIN      
        1 ctgttgctgc tgatgccact gagagctgtg aggaccgtgt cgcgctcaca tggaacaatc
       61 tccggaaaac cctcctggtc catcaggcat ctgaaggcct tttcgataat gataccggcg
      121 ctctgctctc cctgggcagg gaaatgttcc gcctcgaaat tctggaggac attgcccggg
      181 ataaagtcag aactctccat tttgtggatg agatagaagt ctacctggcc ttccagacca
      241 tgctcgcaga gaaacttcag ctctccactg ccgtgaagga aatgcgtttc tatggcgtgt
      301 cgggagtgac agcaaatgac ctccgcactg ccgaagccat ggtcagaagc cgtgaagaga
      361 atgaatttac ggactggttc tccctctggg gaccatggca tgctgtactg aagcgtacgg
      421 aagctgaccg ctgggcgcag gcagaagagc agaagtatga gatgctggag aatgagtacc
      481 ctcagagggt ggctgaccgg ctgaaagcat ccggtctgag cgatgatgcg gatgcggaga
      541 gggaagcagg agcacaggtg atgcgtgaga ctgaacagca gatttaccgt cagctgactg
      601 acgaggtact ggccctgcga ttgtctgaaa acggctcaca actgcaccat tcataatcac
      661 atcgcataaa ccacagaccg gactgactcc ggaaaaacag aggcccgccc ccgggcctcc
      721 ccggattcat ccgtttccct gttcagcctg acagcacgcc ccggcggccg gatgacagac
      781 tccgcttcgg taagcaaagc ggtcttctgt gattccgcca gttgcggctt attcattact
      841 caacgtcaaa cgcccgaatt gaagccaaat catccagacc gctcagctcc tctttcattt
      901 cacgctgacg gcgataaatc tcatcgttgc gatcgacctg cgcctgcacc attgctgccg
      961 ccagttcttc cagttccggc atcgacagtt tcacctgctg attatcggca tcaccccacg
     1021 tcatcacgtc ccgtacaatg tcggatttcg cagccattac caccggataa aggcggccca
     1081 gtgagttggg gccagcattc cagatacgac cgttccattc aaacgtgaac ggcttcgcct
     1141 cctgttctgt gcgccatgct tcaatttcct gacgtctggc ctctctggcc gcttccagca
     1201 tttctggtgt cacagtgaat ggggctatct caccccattt gccactttgc agttcctgcc
     1261 agattcgctg acccgtcggt gcgacatcat cagcggtggc tgtgtaggga actgcctggt
     1321 ccctgtcgtc aaaaaaaacg tcacagtcta ctgcgccact ttcggtataa cggggattaa
     1381 tgattttttt aatttccacg gtgcattcct cacgatgtgc gaataaaaag cccgggcatt
     1441 gcgccagaga
//
LOCUS       DAEEUE010000255         1392 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.261, whole genome shotgun sequence.
ACCESSION   DAEEUE010000255 DAEEUE010000000
VERSION     DAEEUE010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.261"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            63..251
                     /locus_tag="KLK84_004260"
     CDS             63..251
                     /locus_tag="KLK84_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011331.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQDTGG"
     gene            261..>1392
                     /locus_tag="KLK84_004261"
     CDS             join(261..1077,1077..>1392)
                     /locus_tag="KLK84_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HBE2011332.1"
                     /translation="MLSAFTPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLACG
                     TRILGVKEYICDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHL
                     VFTLPDTQWPVFESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHP
                     HVHVSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQRLLKAWSEGLAMPESLSHITT
                     ESQRRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGGSLS
                     FRYLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRA
                     LGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAG"
BASE COUNT          310 a          368 c          430 g          284 t
ORIGIN      
        1 gccgcacgct ccgcagaata tgtatgctct gaggacctgt tttattaagg actcatcctc
       61 ttatgttgac ccgcaaaagt atagataccg ttctgctctc tgttggtgct gagaagctct
      121 cacaacgtga atgggactgg atgaagatgc tcaaacccat ggacccacca cctgcgatgg
      181 tcgcggcctc catcctggag cgccgtggcg acacagcagc ccttacccgg ctacaggata
      241 ccgggggtta atccgtcctc atgttgtccg cttttactcc ccgcccgctg aaacgtctgt
      301 tcacggccaa ccagtgctgg acatccttcc tggatgcggg cggtctgcgc gatatcgagg
      361 ttgaggctgt cacaaaaatg ctggcctgcg gcacgcggat actgggcgtg aaggaataca
      421 tctgcgataa acctgagtgc ccccacgtca gatacgtcac taactcatgc ggcagccgtg
      481 cctgcccgtc ctgcggaaaa aaggccacag acctgtggat agcgacacag ctgaatcgtc
      541 ttcctgactg cgactgggta cacctggtct tcaccctgcc ggacacgcag tggccggtgt
      601 tcgaaagcaa ccggtggctg ctgaatgacg tgtgccgtct ggcggtggag aatctgctgt
      661 atgccgcccg gaaacggggg caggaacccg gtatcttctg cgccatccac acgtatggcc
      721 gtcgtctcaa ctggcatccg catgtacatg tgtctgtaac ctgtggaggt ctgaataagc
      781 atggtcagtg gaaaaagctg agcttcctga aagacgcgat gcgttcgcgg tggatgtgga
      841 atatgcggca acggcttctg aaagcgtggt cagaggggct ggcgatgccg gagtcgttgt
      901 cacatatcac gacggaatca cagaggagaa gcctggtgct gaaagccgga ggaaaatact
      961 ggcatgtgta catgtcgaaa aaaacggccg gcgggaggaa tacggcgcgc tacctgggtc
     1021 gttatctgaa gaagccgccg atagcggcct cccggctggc acattacaac ggagggggag
     1081 cctgagcttc cgttacctgg accacaaaac gggagaaacg gcgacggaaa cgctgacaca
     1141 gcgtgagctg gtcgcgaggc tgaaacagca catcccggag aagtttttta agatggtgag
     1201 gtacttcggg ttccttgcca accgggtgtg tggagagaag ctgccgcagg tgtaccgtgc
     1261 tctggggatg gataaaccgg aaccagtggc gaaagtgtgc tatgcacaaa tggtgaaaca
     1321 gttcctgagt cgtgacccgt tcgaatgcgt gctgtgtggc ggccggatgg tataccgccg
     1381 ggccatcgcg gg
//
LOCUS       DAEEUE010000256         1377 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.262, whole genome shotgun sequence.
ACCESSION   DAEEUE010000256 DAEEUE010000000
VERSION     DAEEUE010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.262"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            1..250
                     /locus_tag="KLK84_004262"
                     /pseudo
     CDS             1..250
                     /locus_tag="KLK84_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            743..>1377
                     /locus_tag="KLK84_004263"
     CDS             743..>1377
                     /locus_tag="KLK84_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBE2011333.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCTN
                     FVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQEL
                     CGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTL"
BASE COUNT          338 a          319 c          420 g          300 t
ORIGIN      
        1 caacacaaag gcaactaata aagatacaga taaagatcta aaaacagatc taacccatcc
       61 caaacccttc ccttccggaa gggagtttcg ggattttgtg gctggagtgc ttgaggggag
      121 attatctggc ggtactgcag cggaattttg taattctgcg gtggttgcgt tgcaggctgc
      181 tggcctggat gtctgtcgtg agtatccggt gccagagcgt ggtgacggtt gcggagggcg
      241 gattgatatc gtcgtgactg acaggaacgg tgtccggtgt gggatcgagc ttgaccgaaa
      301 ttctccgcga cagaaatcac tgctcaaaat cggtgctgtt gaaaccggga tatgtgtctt
      361 gcggcgcagt gatatcgcaa ggcacaccga gcagggaatt ctggttatcg gtggggctgt
      421 tcgccagaaa aaatttgacc cgttgtcagt tgatctgccc gactggttgc cagaaacact
      481 ctggcatgag tgggtccagt tcaggcaggc attgcgaaaa ccgattcgaa cggagctggg
      541 cgctaacggg gcgatacggg aactggaaaa attccgtcag cagggtttta cacctgagca
      601 ggtgattcga cacagcatcg ccaatgaata ccagggtctg ttcgcgccga aaggtgttcg
      661 gcctgagacg ttgctccgac aggttaacac cgtctcgttg ccggacagtg cgatcccgct
      721 aggcttcagg gggtaacaga ccatgaaaaa tattgcgaca ggaggcgttc tggagcgtat
      781 ccgcagactg accccaccac atgtaaccgc cccattcaga acggttgcgg agtggcgcga
      841 gtggcaactt gctgaaggcc agaaacgttg cgaggagatc aaccgcctga atcgtcagtt
      901 gcgggtggaa aaaatcctcc gctccggcat ccagccgttg caccggaagt gttcgtttgc
      961 gaattaccgg gcgcagaacg acggtcagcg acatgcactg agtcaggcga aatccatagc
     1021 tgacgaactg atgacaggct gtacgaattt tgtgttcagc ggtaagcccg gcaccggaaa
     1081 aaatcatctt gcagccgcga ttggcaaccg gctgatggcg aaggggcgta gcgtgattat
     1141 cgtcaccgtg tccgatgtca tgagcgtgtt gcatgagagc tacgacaacg gcaaatccgg
     1201 tgaaaaattt ttacaggagc tttgtggtgt tgacctgctg gtcctggatg aaattggcat
     1261 gcagcgggat acgaaaaacg agcaggtggt actgaaccag attgttgatc gccggacggc
     1321 atcgttacgc ggtgtgggga tgctgacaaa tattaaccat gcagcgatga atacact
//
LOCUS       DAEEUE010000257         1356 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.263, whole genome shotgun sequence.
ACCESSION   DAEEUE010000257 DAEEUE010000000
VERSION     DAEEUE010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.263"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(41..100)
                     /locus_tag="KLK84_004264"
     CDS             complement(41..100)
                     /locus_tag="KLK84_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBE2011334.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(339..1247)
                     /locus_tag="KLK84_004265"
     CDS             complement(339..1247)
                     /locus_tag="KLK84_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011335.1"
                     /translation="MTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAERQIQE
                     TEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEARANL
                     ALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQA
                     AAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEP
                     MKSIDGIKIIQVDGLNSGSAAGDANTGNVEGGNLAEQALSAALSYRTQAPLIDSLLNE
                     IGVSGGSLAALTSPLISTTPVAENVK"
     gene            complement(1281..>1356)
                     /locus_tag="KLK84_004266"
     CDS             complement(1281..>1356)
                     /locus_tag="KLK84_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE2011336.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT          274 a          361 c          326 g          395 t
ORIGIN      
        1 acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta
       61 cgagtaacaa cacaatcagt atgatgacga gcttcatcat aaccctttcc ttctgtaagg
      121 ccccttcctc gggaggggct ttcccgtttc agccctttgc tgactccccg attgttgatg
      181 agcgccggac ctcgcgcaac gctttcccag aggcagtttt gccttccccg agcggtcacg
      241 caaaacgttg caacgacctg atcatttgca gtttttagcg gaacgcagat ataaaaaatc
      301 cccggctttt tgtgccaggg acgttcatag ctgaaaattt attttacgtt ttcggcgacg
      361 ggagttgttg agattaaggg tgaagtcaat gccgccagtg aaccgcctga aacgccaatt
      421 tcattgagca aggagtcaat cagcggagcc tgtgtgcggt aagagagagc ggctgataat
      481 gcttgttccg ctaggtttcc gccctccaca ttacccgtgt ttgcatcacc cgcagcgctg
      541 ccactattca gaccatcgac ctgaataatc ttgataccgt caatagattt catcggctca
      601 acggattttt ctattaccgc aggtagcgcc tgcaacaagg ccagtttgaa tttaaggctg
      661 gtttgttcat cagaaagtac gttgatagca tcgttcagcg cacgttgtgc ttctgcttcc
      721 gccagaccct ttttacgtgt agcttcggct aactcaacga tagccgccgc ctgcatctct
      781 gcggcttctt tttctgcttt cgcccgcacg gtcagttcaa ccgctttggt ttctgcatcc
      841 tgcgcggcag cgattagggc aacttgttta gcacgatcgg cttctgcggt ctggcgagtg
      901 gtttctacgt tttgttgcgc acttactgct tctgcgagtg caaggttagc acgcgcttct
      961 gcctgggact gttgttccga tttggcggca atagcgatcg atttcgtctg gttggcgatt
     1021 tcggtgacct gctgctgttc gatctcttta atacgaactt cacgttcagc ctcaaccttt
     1081 cttgagcgga cggcctgctc gcgctcgatc tccgtttcct gaatttgtcg ttcagccaga
     1141 attcgcgtct gctctgcttc acgacgacgt tcagcttcaa aagccgcaat acgtgcattc
     1201 tgttcggcag tacgggtttt aacctgctgc tcctgctcaa gcgtcataaa cgcttcttgt
     1261 tgttcggtaa tgactccaac ttactgatag tgttttatgt tcagataatg cccgatgacc
     1321 ttgtcatgca actccaccga ttttgagaac gacagt
//
LOCUS       DAEEUE010000258         1354 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.264, whole genome shotgun sequence.
ACCESSION   DAEEUE010000258 DAEEUE010000000
VERSION     DAEEUE010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.264"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..226)
                     /locus_tag="KLK84_004267"
     CDS             complement(<1..226)
                     /locus_tag="KLK84_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE2011337.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWATICSIAPKIGCTP
                     ETLRVWVRQHERDTEGGDGGLTTAERQRLKE"
     gene            303..845
                     /locus_tag="KLK84_004268"
     CDS             303..845
                     /locus_tag="KLK84_004268"
                     /inference="COORDINATES: protein motif:HMM:NF024120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011338.1"
                     /translation="MIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDNYQAIQ
                     NAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAKSGLTGS
                     VLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVMVNEVET
                     ALFNARALALKIYSMSGWNL"
     gene            1072..>1354
                     /locus_tag="KLK84_004269"
     CDS             1072..>1354
                     /locus_tag="KLK84_004269"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HBE2011339.1"
                     /translation="MEQLDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIY
                     FRTPASTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTG"
BASE COUNT          373 a          284 c          330 g          367 t
ORIGIN      
        1 gctctttcag acgctgacgt tcagcggtgg tgagccctcc atcaccgccc tcggtatccc
       61 gctcatgctg gcgaacccag acacgcagag tctccggcgt acagccaatc tttggggcaa
      121 tggaacaaat tgtcgcccat tgtgagtcat attcgccctg actttccaga accatacgga
      181 ctgcccgttg acggacttcg ggggaaaaac gagtattttt agtcatcctg tttacctctt
      241 tctcaggaag tttagtctcc aggattcccg gggcggttca ggataacacc aaaggcgatg
      301 ccatgattgg cgtcaagcag cctttaccaa aagcagtttt aagaactcag catgacaaaa
      361 ataaagaagc aataagtatc ctggattttg gtgttattga tgatggtgtg acagataatt
      421 accaggcaat acaaaatgct atagatgccg ttgcttcact accctccggc ggggagctgt
      481 ttatccctgc gagcaaccaa gcggtggggt atattgttgg atccactttg cttattcctg
      541 gcggtgttaa catcagagga gttggtaagg catcgcaact ccgagcaaaa agcggactta
      601 caggatctgt gttaaggctg tcttatgatt cagacactat cggccgttat ctgagaaata
      661 tacgagtaac tggtaataac acctgcaatg gtattgacac aaacattaca gcagaagact
      721 ctgtcatcag acaggtttat ggctgggtat ttgataatgt aatggtgaat gaagttgaaa
      781 ccgcattatt taatgcaagg gctctggcac tcaaaattta tagcatgtca ggctggaacc
      841 tgtagagtcg gtcttcactt tttaggccag tgcgtaagtg ttagtgtcag ctcctgccat
      901 ttcagcagag gaaattattc tgctgatgaa agctttggca tcaggattca gcctcaaacg
      961 tatgcgtggt cgtcagaggc agttaggtca gaagcaataa ttttagacag tgagaccatg
     1021 tgcattggtt ttaaaaatgc cgtctatgtt catgattgcc ttgatttgca tatggaacaa
     1081 ctggatttag attattgcgg ctcaacaggc gtggttatag agaatgtaaa cggaggattt
     1141 tctttctcaa actcatggat agcagcagat gccgatggca ctgaacaatt tacggggata
     1201 tattttagaa cgccggcctc aacgcagtca cataaaattg tcagtggtgt tcatatcaac
     1261 actgcaaaca aaaacacggc tgcaaacaat caaagtatag cgatagaaca gtcggcgatc
     1321 ttcgtctttg taagtggttg tacgttaact ggtg
//
LOCUS       DAEEUE010000259         1347 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.265, whole genome shotgun sequence.
ACCESSION   DAEEUE010000259 DAEEUE010000000
VERSION     DAEEUE010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.265"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..636)
                     /locus_tag="KLK84_004270"
     CDS             complement(<1..636)
                     /locus_tag="KLK84_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HBE2011340.1"
                     /translation="MILSVFLGQPALTYAGVVIGSTRVVYLSNNADKSISVFSKEEKI
                     PYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEKILRIIHTKGVSLPDDRESVFWL
                     NIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAEN
                     PNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPANSAGGQMTWRFINDYGA"
     gene            complement(750..1322)
                     /locus_tag="KLK84_004271"
     CDS             complement(750..1322)
                     /locus_tag="KLK84_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE2011341.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGKIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          370 a          310 c          284 g          383 t
ORIGIN      
        1 tgcgccgtaa tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa
       61 aggtaacgtg agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac
      121 gatgacatcc atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc
      181 ttcccggcgc catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg
      241 tcgccagaac agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt
      301 gctcgctgat ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg
      361 caatgacaca cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg
      421 tgatactggc ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc
      481 tacccaagcc tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt
      541 atcagcatta ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt
      601 taatgctggt tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac
      661 tgttgtttta aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa
      721 gccaactgaa taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa
      781 tcagcgttcg ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac
      841 ttcatttcag ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta
      901 acctgcgaat tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct
      961 tgtcctgtac cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg
     1021 tcgccgtcgc cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt
     1081 gccgggcaat tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct
     1141 gaaaatgcat ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta
     1201 acttcacaag tagtgtcgac aattttaccg acgaagttaa caacaccatc ttctgccagt
     1261 gcatgcccgg ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc
     1321 ataaaaactc cattgatata atatatc
//
LOCUS       DAEEUE010000260         1284 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.269, whole genome shotgun sequence.
ACCESSION   DAEEUE010000260 DAEEUE010000000
VERSION     DAEEUE010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.269"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(80..346)
                     /gene="mobC"
                     /locus_tag="KLK84_004272"
     CDS             complement(80..346)
                     /gene="mobC"
                     /locus_tag="KLK84_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBE2011342.1"
                     /translation="MPVLTDEEVNTVRESCRQLGAIGRNLNQVARALNIEFRESDKLK
                     QEAIEKLAERIDQHLDHVSELFDKTWSRWHDCHFQKTTAPVDWA"
     gene            complement(567..1040)
                     /locus_tag="KLK84_004273"
     CDS             complement(567..1040)
                     /locus_tag="KLK84_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HBE2011343.1"
                     /translation="MKVSLMAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
BASE COUNT          313 a          337 c          288 g          346 t
ORIGIN      
        1 cacgagtatt gagcatagtc gagattggtg cagatcactt ctgatattga actgtcagga
       61 gctggctgca caacagccat tacgcccaat caactggtgc agtcgtcttc tgaaaatgac
      121 aatcgtgcca ccggctccag gtcttatcga acagctcaga cacatggtcc aaatgctggt
      181 cgatccgttc ggccagtttt tcgatggcct cttgcttgag cttgtcactt tccctgaact
      241 cgatgttcaa ggccctggcc acctggttga ggttccggcc gatagctcca agctgccggc
      301 acgattcccg tacggtgttc acttcttcat cagtgaggac cggcatccgg ttcaacgacc
      361 gcagtcattt ctgtacctaa acagtcctgc gaatatgtga gttttgacct aatgagccac
      421 aaaaactcat tttcgcccac gataattttt ctacttcctt tctttggaat gctcatttca
      481 atattcacat aacgtcgcta tcaatcgcgg aaaaggcgta gtgcgcgaag cgaacgaagc
      541 tttttgccgt cgattgcata gctttgttaa ccctttttcc aaatttgata gcaatagtta
      601 atgtttgaag taaagtgttg ctcaaaaaca acttcgaagg tatttggaat actcgggaag
      661 aaaacatccc cctctggctc gatgtcgatc gtcgataagt ggagcgtaga ggccatgggt
      721 aatgtttctc ggtaaatttc tccgccacca gacactataa cgtgaccggt gaattcagct
      781 agcctgtcca tggcctcttc gattgactga aatacaacta cattgtcatc atttgatgtc
      841 caacctgagc gggtaacgac cgcgtatttc ctattgggga gtgcgcccat agattcaaac
      901 gtcttgcgac ccaccagaag ccactgattg taggtcaatg ctttaaaaag tagctgctcc
      961 cctttcgcgg accagggtat gtctggaccg caaccaatca cgccgttttt cgctttcgca
     1021 gccatcaatg atactttcaa ggttctcatc ctgggttaac tttgttttag ggcgactgcc
     1081 ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc
     1141 ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa cagtcataac aagccatgaa
     1201 aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga
     1261 gcgcatacgc tacttgcatt acag
//
LOCUS       DAEEUE010000261         1186 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.270, whole genome shotgun sequence.
ACCESSION   DAEEUE010000261 DAEEUE010000000
VERSION     DAEEUE010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.270"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            1..520
                     /locus_tag="KLK84_004274"
                     /pseudo
     CDS             1..520
                     /locus_tag="KLK84_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            544..1186
                     /locus_tag="KLK84_004275"
                     /pseudo
     CDS             544..1186
                     /locus_tag="KLK84_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
BASE COUNT          328 a          265 c          324 g          269 t
ORIGIN      
        1 cgggtaatcc gaaaacagca gaacaatatc agatgacaaa acgatattcg gtcacctggc
       61 ttttttcaga agacggaaaa aactggtatg aagagctgaa gaatttcgcc aggacacaat
      121 taaaataact tacaccggag acggtcgcgt ggtgtgggtc ggtaaggatg tgacaggcat
      181 tgaaccccgt aacgccagtg ttattgaagt tcctgatatt accgccaacc gccgcattac
      241 cgcgccgggt tactggtttt accgcaatga tgaatttgtc tttgactaca gactcaaagc
      301 ggaagatgag cgtgatgccc ttctggctca ggtcagtgcc cggacagggg aatgggaaga
      361 agacctgttg ctggggctaa tcagcgacga agaccgggaa aaactgaaag cgtaccgcat
      421 ttacgcgaaa tcgctgcagg cgatggattt cagtgtcatc actgataaaa cctcatacaa
      481 cgccattgaa tggcccgctt ctccggaaat ttcttcctga tttaatttat cgcgagaaaa
      541 actatgtctg tagtgatatc aggtgcgctg actgatggcg caggtatctc catgtccgga
      601 taccatatta ttctgaaatc ccgggtaaac accccggaag tggtgatgca cactgttgct
      661 gatgtgatga caggaaacga tggtgaatac tgtttccatg cgcggactgg aaaatatggt
      721 gtgtatctga aacaggactg gcacaacgag tacaacgttg gcgacattgc tgtatatgag
      781 gactcaaagc ccggtacgct gaatgacttt ctgattgctc ctgatgaggg cgacctgaaa
      841 ccagatgtcg tcaaacgctt tgaggaaatg gtggcgcagg cgcagcagag caggccaaaa
      901 atgacattga tgctgcgctg accggcactc tgaaaacggc taaccatctg tctgaaatcg
      961 cagcagcagg cgaaaaggca caacagaagt cccgggataa tctggggctg aaaagtgcgg
     1021 ccacgatgga agcacagagc gacatttacg accggacaaa aggccgtctg gcgatacccg
     1081 gcgcattcgg ctttgggtgt gcttttctgc ctgaagatgt tatccgtttt gacactaaga
     1141 gtgatttcct ggcctgggta aggaatgcgc tgccaggtga atattc
//
LOCUS       DAEEUE010000262         1162 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.271, whole genome shotgun sequence.
ACCESSION   DAEEUE010000262 DAEEUE010000000
VERSION     DAEEUE010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.271"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            117..1162
                     /gene="cadB"
                     /locus_tag="KLK84_004276"
                     /pseudo
     CDS             117..1162
                     /gene="cadB"
                     /locus_tag="KLK84_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
BASE COUNT          247 a          256 c          298 g          361 t
ORIGIN      
        1 tctttaggat taatcctttt ttcgtgagta atcttatcgc cagtttggtc tggtcaggaa
       61 atagttatac atcatgaccc ggactccaaa ttcaaaaatg aaattaggag aagagcatga
      121 gttctgccaa gaagatcggg ctatttgcct gtaccggtgt tgttgccggt aatatgatgg
      181 ggagcggtat tgcattatta cctgcgaacc tagcaagtat cggtggtatt gctatctggg
      241 gttggattat ctctattatt ggtgcaatgt cgctggcgta tgtatatgcc cgactggcaa
      301 caaaaaaccc gcaacaaggt ggcccaattg cttatgccgg agaaatttcc cctgcatttg
      361 gttttcagac aggtgttctt tattaccatg ctaactggat tggtaacctg gcgattggta
      421 ttaccgctgt atcttatctt tccaccttct tcccagtatt aaatgatcct gttccggcgg
      481 gtatcgcctg tattgctatc gtctgggtat ttacctttgt aaatatgctc ggcggtacct
      541 gggtaagccg tttaaccact attggtctgg tgctggttct tattcctgtg gtgatgactg
      601 ctattgttgg ctggcattgg tttgatgcgg caacttatgc agctaactgg aatactgcgg
      661 ataccactga tggtcatgcg atcattaaaa gtattctgct ctgcctgtgg gccttcgtgg
      721 gtgttgaatc cgcagcagta agtactggta tggttaaaaa cccgaaacgt accgttccgc
      781 tggcaaccat gctgggtact ggtttagcag gtattgttta tatcgctgcg actcaggtgc
      841 tttccggtat gtatccgtct tctgtaatgg cggcttccgg tgctccgttt gcaatcagtg
      901 cttcagctat cctcggtaac tgggctgcgc cgctggtttc tgcattcacc gcctttgcgt
      961 gcctgacttc tctgggctcc tagatgatgt tggtaggcca ggcaggtgta cgtgccgcta
     1021 acgacggtaa cttcccgaaa gtttatggtg aagtcgacag caacggtatt ccgaaaaaag
     1081 gtctgctgct ggctgcagtg aaaatgactg ccctgatgat cctcatcact ctgatgaact
     1141 ctgccggtgg taaagcatct ga
//
LOCUS       DAEEUE010000263         1118 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.272, whole genome shotgun sequence.
ACCESSION   DAEEUE010000263 DAEEUE010000000
VERSION     DAEEUE010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.272"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            87..>1118
                     /locus_tag="KLK84_004277"
     CDS             87..>1118
                     /locus_tag="KLK84_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBE2011344.1"
                     /translation="MSITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQP
                     LGNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDR
                     KLSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAH
                     QSQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDG
                     TLALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTS
                     QLVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPVPFGAVISYSKDTENMAGIVGEDG
                     IAYVSGLSAE"
BASE COUNT          347 a          208 c          287 g          276 t
ORIGIN      
        1 ctccaatgac tagtctaaaa actagtatta agactatcac ttaaataagt gatactggtt
       61 gtctggagat tcagggggcc agtctattgt ctataaccac gaaccgttat gcatcaggtt
      121 atgccacact gactgaagca gtaagtgcac aggatgaacg gaacagaaaa agagataaga
      181 atagtcacga tggctctacg atatccttat cccagcccct tggtaatatc gggaatttga
      241 acttcaatac cacgaggtat aattcttccc ggggcacagg aaacacgcgg tctaccagcc
      301 tgagttatag tacggtatgg agaggcataa cattctccat taactgggca aaaaacgact
      361 tgctgacatc gcataaatgg aaagtagatc ggaaattaag cgtgggtatt tcagtgccat
      421 tatcgctggg tgacgaaaat caaatttatg cgtcttcaca gatgagtcgt tcgggagagc
      481 agggtaataa ctatcaggtt tcattatcag gtcagaatag tggtggtgta tggtgggatg
      541 tagcgacaaa tataactaac gctcatcagt cacaaccgaa gagcacgatg aacattgtgc
      601 aagttggcaa aaatggatca tatggtcagt ttagtagcca ttacagctcc agcgaaaaca
      661 tgaaacagct aggtgcaaat ttaagcggtg ggatacttat tacaagggat ggacttacat
      721 ttggtcaaaa tgtggatggg acattagccc ttatagaggc acccggagca acgggggtta
      781 acgttaatgg ctggccggga ctgagtactg attttcgtgg gtatgcaata ctgccagtgc
      841 agccataccg aagagacgat gttattctgg atgaaaaaac gataggtaag aattacgact
      901 taccacaaac tagtcaattg gttgtaccga cagctggagc cgttgtgcct gcgacattgg
      961 cagtgaagag cggggataaa ggattagtaa ccttaaaaca gaaagagggt aaacccgtcc
     1021 ccttcggcgc tgtgattagt tatagcaaag acactgaaaa tatggccggc attgtaggtg
     1081 aagatggtat tgcttatgtt tctggcttga gtgcggaa
//
LOCUS       DAEEUE010000264         1110 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.273, whole genome shotgun sequence.
ACCESSION   DAEEUE010000264 DAEEUE010000000
VERSION     DAEEUE010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.273"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            1..1019
                     /locus_tag="KLK84_004278"
                     /pseudo
     CDS             1..1019
                     /locus_tag="KLK84_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          312 a          271 c          259 g          268 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggttag
//
LOCUS       DAEEUE010000265         1102 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.274, whole genome shotgun sequence.
ACCESSION   DAEEUE010000265 DAEEUE010000000
VERSION     DAEEUE010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.274"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            52..>1102
                     /locus_tag="KLK84_004279"
     CDS             join(52..301,301..>1102)
                     /locus_tag="KLK84_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
                     /protein_id="HBE2011345.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRELRKKLQRIEMENEIFKKGYRALDVRLPEQFSII
                     GKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGAR
                     SIATMATRRGYQMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNYLERQFAVT
                     EPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETR
                     GKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWM
                     PVVGYVSFSEAAHAIT"
BASE COUNT          303 a          273 c          310 g          216 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctcccccat taccccggaa
      241 caaattgaaa tccgtgagct caggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttgaaaggc agttcgccgt gaccgagcca aatcaggtgt
      721 ggtgcggtga tgtgacctat atctggacgg gtaagcgctg ggcgtacctc gccgttgttc
      781 tcgacctgtt cgcaagaaaa ccagtgggct gggccatgtc gttctcgccg gacagcaggc
      841 tcaccatgaa agcgctggaa atggcatggg aaacccgtgg taagcccggc ggggtgatgt
      901 tccacagcga tcagggcagt cattatacga gcaggcagtt ccggcagtta ttgtggcgat
      961 accagatcag acagagtatg agccggcgcg gaaactgctg ggataacagc ccaatggaac
     1021 gcttcttcag gagtctgaag aacgaatgga tgccggtggt gggttacgta agcttcagcg
     1081 aggcagctca cgccataacg ga
//
LOCUS       DAEEUE010000266         1095 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.275, whole genome shotgun sequence.
ACCESSION   DAEEUE010000266 DAEEUE010000000
VERSION     DAEEUE010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.275"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            482..>1095
                     /gene="uacT"
                     /locus_tag="KLK84_004280"
     CDS             482..>1095
                     /gene="uacT"
                     /locus_tag="KLK84_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HBE2011346.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKG"
BASE COUNT          263 a          253 c          236 g          343 t
ORIGIN      
        1 gacagcctat acattccgca gcttcagcag cgataaactt attcatttgc atccctttca
       61 tttaatgagt tatgtcttct caaatttcgc gatgcaataa acgggcatat attaggtagc
      121 atgaccgttt taattataat tttgcgactg agttcaaatt attgcccctc aatctatgcc
      181 gctttcatta tttcaacatt gccgatcaaa agagcgctat ccagataaaa acgattaaat
      241 gcgagagtgc gacatgccag aatgattaat gaaatatcaa tatgagaaaa ttacagataa
      301 ttccattgtc aggttctctt gtcattttcg tggtttaagc acgattatgt gattgcctcc
      361 gcaattcctg tctctaactc cccttcctcg caaaaactgg cactccacga gcatgtgttt
      421 agacagtttc attaacgtaa acggttgctt tttattctgg cgggcgaaag gagaaacact
      481 gatgagcgcc atagattccc aacttccctc atcttctggg caagaccgcc caactgatga
      541 ggttgaccgc atattatcac caggaaagct gatcatactc ggtctgcaac acgtccttgt
      601 catgtacgca ggtgcagtcg ctgttcctct tatgattggt gaccgactgg gcctctcaaa
      661 agatgctatt gcgatgctca ttagctcgga tctcttttgc tgcgggatcg tcacattatt
      721 gcaatgtatc ggtatcggcc gctttatggg gatccgcctg ccggtgatta tgtcggtgac
      781 ctttgctgct gtaacaccaa tgatagccat tgggatgaac ccggatatcg gcctgctggg
      841 gatatttggt gccactatcg ccgcgggttt tatcaccaca ttattagcgc cacttattgg
      901 tcgcttgatg cctttattcc cgccactggt taccggtgtg gttattactt ctattgggct
      961 tagcatcatt caggtgggta ttgactgggc cgctggaggt aaagggaatc cgcaatatgg
     1021 taatcccgtt tatttaggta tctcctttgc cgtcttaatt tttatcttgc tcattactcg
     1081 ctatgcgaaa ggatt
//
LOCUS       DAEEUE010000267         1080 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.276, whole genome shotgun sequence.
ACCESSION   DAEEUE010000267 DAEEUE010000000
VERSION     DAEEUE010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.276"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..307
                     /locus_tag="KLK84_004281"
     CDS             <1..307
                     /locus_tag="KLK84_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011347.1"
                     /translation="LNSVVMPLRYLWVFVAFIAVVRLAQKYKPEYVFIRNKPLAMTVG
                     IWCFAFTACACLTGIFPKMEAFTAEWTFQLALNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(308..952)
                     /locus_tag="KLK84_004282"
     CDS             complement(308..952)
                     /locus_tag="KLK84_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE2011348.1"
                     /translation="MTPTGTLKINAARVAARIFLMPLLVGFTREYPDIKVELTTDDSL
                     IDIVQQGFDAGVRLSGIVEKDMISVAIGPPVKLCVAAMPEYFARYGKPRHPHDLLNHQ
                     CVVFRYPSGKPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGIGYLLYEQIKEYL
                     DTGRLECVLEDWITERPGFQVYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT          278 a          247 c          256 g          299 t
ORIGIN      
        1 tcttaactcg gtagtgatgc cgctgcgtta tttatgggta tttgttgcat ttattgcagt
       61 cgttcgcctg gcgcagaaat ataaaccaga atatgtcttt attcgtaata agcctctggc
      121 aatgaccgtc gggatttggt gttttgcctt taccgcctgt gcctgtttga cggggatctt
      181 cccgaaaatg gaagccttca ctgcagagtg gaccttccag ttggcgctga atgttgcaac
      241 gccgtttgtg ctggtaggat taggactgat attcccgctg ctggcgcgta aagcgaatag
      301 caaataatta ttgtggtcgg tgacgctgac tctggcagat ctgacccgtt tttacataat
      361 cgagaaatgc gcgcaagccg caggacatat actggcggtt tgggtaatag acttggaagc
      421 ctggacgctc tgtgatccag tcttccagta cacattctaa acgaccagta tcaaggtact
      481 ccttgatctg ttcatagagc agatacccga taccagcacc catcaaaacg gcctccagtt
      541 cagcatcaac atcatccaga atgatatttc ctgcgacagc aatttccagc tcctttgcaa
      601 attgccagtg gaaaggtttg ccgctgggat aacgaaaaac aacacattga tgattaagca
      661 gatcatgagg atggcggggt ttgccatatc ttgcaaagta ctctggcatt gctgctacac
      721 ataatttcac tggtgggccg atcgccacgc tgatcatatc tttttcgacg atgccactaa
      781 gtcgaacgcc agcgtcaaag ccctgttgga cgatatcgat aagagaatcg tcagttgtga
      841 gctctacttt aatatcagga tattcacgtg tgaaaccaac cagtaaaggc attaaaaata
      901 tacgtgcagc tacccgggcg gcattgattt tgagcgttcc tgtcggtgtc agacgaaaat
      961 cattcatctc atctaacatg atctgaattt cgtcgaaagc tgggcgcaat cgttcataga
     1021 gattagaacc cgcttccgtt aacgatacgc ttcgggttgt ccgattgaat aagcgaattt
//
LOCUS       DAEEUE010000268         1066 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.277, whole genome shotgun sequence.
ACCESSION   DAEEUE010000268 DAEEUE010000000
VERSION     DAEEUE010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.277"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(570..749)
                     /locus_tag="KLK84_004283"
                     /pseudo
     CDS             complement(570..749)
                     /locus_tag="KLK84_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            843..>1066
                     /locus_tag="KLK84_004284"
     CDS             843..>1066
                     /locus_tag="KLK84_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE2011349.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAA"
BASE COUNT          285 a          193 c          277 g          311 t
ORIGIN      
        1 ttagggtagt cactatattt gcgactgagc tggtcgctat ttttaaccag gctgaaaaat
       61 tgagtcaata tatcgaaagc ggtagttggc gggcataagt atgtaatgat gatgctgcac
      121 tggtgttgaa tattattcac gccaaacttt cctcattgag caatgtcgtc gcaagtggcc
      181 tcaatgattt tagaccatta tcagaggtcc ggagcggcgg gaagagattt attgctgtgg
      241 aataattgtg gtactgatga ttatttatat cccggattgg tagggaaaag cttttttcat
      301 gcactatttt tgcaaatatt tctatatggg aagtggtttt gttacagatt aaatttataa
      361 agaaatgcat ttggggtgtt gggttgaaga gtagctggta gatgttattg tgccagcagg
      421 ggcaggggaa tgtgccgtat cgatatgctt tggcaattgc gtgtgccgca gatggagatg
      481 aaatgccaat aaaaaagcct gagttcgatg agggacgaac tcaggctggt gttatgtttt
      541 acagtaacac gcctcggcgc gttggctgat tagaactggt agacgatacc aactgccagt
      601 tggtcgtcgg tgttcagtcc atgagcgaga acataatcac tcttatcaag caggttgatc
      661 tgataagcgg tatatatgtt catattctta ttgaagtaat accaggtacc gatttcaata
      721 tagttcagac gatcagaatc tttgtaacct gtagactggc cccctgaatc tccagacaac
      781 caatatcact tatttaagtg atagtcttaa tactagtttt tagactagtc attggagagc
      841 agatgattga tgtcttagga ccggagaaac gcagacggcg tactacacag gaaaagatcg
      901 ctattgttca gcagagtttt gaaccgggaa tgacggtctc ccttgttgcc cggcaacacg
      961 gtgtggcagc cagccagtta tttctctggc gcaagcaata ccaggaggga agtcttactg
     1021 ctgtggccgc aggagaacag gtcgttcctg cctctgaact tgctgc
//
LOCUS       DAEEUE010000269         1041 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.278, whole genome shotgun sequence.
ACCESSION   DAEEUE010000269 DAEEUE010000000
VERSION     DAEEUE010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.278"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..769
                     /locus_tag="KLK84_004285"
     CDS             <1..769
                     /locus_tag="KLK84_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBE2011350.1"
                     /translation="QFATDNGYTSGNPFNGLTPLKKSKIEPDPLTRDEFIRFIEACRH
                     QQTKNLWIIAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGR
                     TIHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSG
                     PHYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQM
                     VFNVYGAWMKDNNHEQIELLNRRLSESVPCMPHKKPG"
     gene            790..>1041
                     /locus_tag="KLK84_004286"
     CDS             790..>1041
                     /locus_tag="KLK84_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBE2011351.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLTYLQS"
BASE COUNT          337 a          232 c          234 g          238 t
ORIGIN      
        1 tcaatttgca acagataatg gttatacatc aggaaatcca tttaacggtc tgacaccatt
       61 aaaaaagtcc aagatagaac cagatcccct cacccgtgac gaatttattc gttttattga
      121 ggcttgccgt catcaacaaa caaaaaacct gtggattatc gctgtataca cgggtattcg
      181 tcacggggag ctggtatcac tggcctggga agatatagac cttaaagcga ggactataac
      241 catccgccgg aattatacaa aactaggcga attcactcca ccaaaaactg atgctggtac
      301 cggaagaacg attcatctag ttcaaccagc tattgatgct cttaaaagcc aagcggaaat
      361 gaccatgctt ggaaaacagc attctgtaga ggtaaagcag agggaatatg ggagaagtac
      421 tgtgcataaa tgcacttttg tttttagtcc tcaggtaata aaacagcggc agttttccgg
      481 accgcactat aaggttgact ccatcaggga gtcatggaca agtatcttaa aacgcgcagg
      541 tctgagacac agaaaatcgt atcaatccag gcatacctat gcatgctggt cacttgccgc
      601 aggagctaat cctagtttta tcgcaagcca gatgggccac acaaacgcac aaatggtatt
      661 caatgtttac ggagcatgga tgaaagacaa caatcacgaa cagatagaac ttcttaacag
      721 aagactatct gaaagtgtcc catgcatgcc ccataagaaa ccggggtaaa ataaaaactt
      781 gcaaaatcaa ttggtttacc tttaatccct gtcacgttac gcgcgtggca gaggcgttac
      841 ggattgctga aaccgcaacg gacagacggc ggtcatcggc tgttcaacga tgccgatatt
      901 gaccggatcc gcgagatcaa acgctggatc gacaacggcg tgcaggtcag caaagttaaa
      961 atactgctca gtaatgaaaa tgttgatgtg cagaacgtct ggcgcgatca gcaagaaaca
     1021 ttactgactt acctgcaaag c
//
LOCUS       DAEEUE010000270         1021 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.279, whole genome shotgun sequence.
ACCESSION   DAEEUE010000270 DAEEUE010000000
VERSION     DAEEUE010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.279"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(50..190)
                     /locus_tag="KLK84_004287"
     CDS             complement(50..190)
                     /locus_tag="KLK84_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011352.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            329..1000
                     /gene="queE"
                     /locus_tag="KLK84_004288"
     CDS             329..1000
                     /gene="queE"
                     /locus_tag="KLK84_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HBE2011353.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          296 a          206 c          237 g          282 t
ORIGIN      
        1 acatacgaaa gtgaacatta gtatgaaata gcgcattata aatttctcat taaatctgga
       61 taaataaatt gacggcggca tcggtaattt tagattcgtt atcattaaaa actaatacag
      121 aaaaaataaa tataattttg gtgaatgttt tgacatgatt aaatccattt tctttatttt
      181 cttctgacat aatacgttac tccctttaat tattaacgat atgtttcgta tttttcttta
      241 aatctgcatg taacagaatg ttaataagac atgattgttg cggcaggatc tgcaataatt
      301 aacaattatc cacttcacag agaatgctat gcagtacccg attaacgaga tgttccagac
      361 cctgcaaggt gagggttact ttaccggcgt ccccgccatt tttattcgtt tacagggatg
      421 cccggttggc tgtgcctggt gcgacaccaa acacacctgg gaaaagcttg aggatcggga
      481 agtctccctt ttcagcattc tggcgaagac caaagagagt gataagtggg gggcggcgag
      541 cagtgaagac ttgctggctg tcattggtcg tcagggatat accgcgcggc atgtggtgat
      601 tacgggtggt gagccttgca ttcatgattt gctgccactg actgacctgc tcgaaaagaa
      661 cggttttagc tgccagatcg aaaccagtgg aactcatgaa gtacgctgca caccgaatac
      721 ctgggttacc gtatcgccaa agctgaacat gcgcggcggc tatgaagtgt tgtcacaggc
      781 actggagcga gccaacgaaa tcaagcatcc agtggggcgc gtacgcgata ttgaagcact
      841 ggatgaacta ctggcaacgc tgaccgatga taaaccgcga gtcattgcac tgcagccgat
      901 tagccaaaag gatgatgcca cacgattgtg cattgaaacc tgtattgcgc gtaattggcg
      961 tttgtcgatg caaacacata aatatttaaa tattgcctga ttacacattt ataagcgtta
     1021 t
//
LOCUS       DAEEUE010000271         1003 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.281, whole genome shotgun sequence.
ACCESSION   DAEEUE010000271 DAEEUE010000000
VERSION     DAEEUE010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.281"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..87
                     /locus_tag="KLK84_004289"
     CDS             <1..87
                     /locus_tag="KLK84_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HBE2011354.1"
                     /translation="RMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            102..524
                     /locus_tag="KLK84_004290"
     CDS             102..524
                     /locus_tag="KLK84_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HBE2011355.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            582..938
                     /locus_tag="KLK84_004291"
     CDS             582..938
                     /locus_tag="KLK84_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011356.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
BASE COUNT          288 a          201 c          276 g          238 t
ORIGIN      
        1 cgcatggtca tgaacggcgg gcgctgggtt aattttaact gggagagctg gcgtccgaat
       61 gttagccatt cgagggttgt taagtagttt caggaggatt tatggcgaaa ccttttactc
      121 ccgaacagcg ggaagaactg aagacgcgaa ttgtggaact cgtgcatcag gacggtcggg
      181 tcacgattcg gcagttgtcc gatgaaacag gtatcagtcg tgcgtctgtc ggtcgcttat
      241 gcatagaact ggtcgcaagt ggtgatgtat ataattctgg ctacggctta ttcccgtctg
      301 aacaggctcg caaggactgg caaagcgccc gcaaaaaact ctcgagagta aaggtgagga
      361 aaccggttgt tgttgatccg gaccttatct ggtcattacc tgacggagaa atacgccgct
      421 acgacaggcg cctgaatata atctgtcgcg agtgccggaa gagcgaagct atgcagcgtg
      481 tactggcttt ctatcagggt aattttcagg aggcgatact gtgaatgaaa ttagctatca
      541 ggcttcaatt accgctggca ttcgcatcaa aggagaggag catggaaata aaaccagaag
      601 atgagttaag caatatcgtt ttatttccga taaaagagga tgaccctcgt aatcaggtta
      661 attttcttta taagccatcg gaaagaccat attgccatca cgcctctgta cgggttgacg
      721 aaaaagagcg tcaggtccgc tgtaaaatct gcggtgcggt tgtggagccg tttgactgga
      781 tgctctctgt ggcaaaaaga gaaaccagac tggcagatga tgtaaggcta ttgcgccagg
      841 aggaacagga aaggcggaga aatatagaaa agctgataca gattgagcgt aacgcgaaag
      901 cgcggatacg cagggcgaca aaatccagaa ctgaataatt aaatttagca ctgttaaaaa
      961 tttaatcctt aaccggaggg atttctgcac cctcaaatca tca
//
LOCUS       DAEEUE010000272          983 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.284, whole genome shotgun sequence.
ACCESSION   DAEEUE010000272 DAEEUE010000000
VERSION     DAEEUE010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.284"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="KLK84_004292"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="KLK84_004292"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HBE2011357.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          259 a          220 c          222 g          282 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcgct ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ata
//
LOCUS       DAEEUE010000273          933 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.285, whole genome shotgun sequence.
ACCESSION   DAEEUE010000273 DAEEUE010000000
VERSION     DAEEUE010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.285"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(55..243)
                     /locus_tag="KLK84_004293"
     CDS             complement(55..243)
                     /locus_tag="KLK84_004293"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011358.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
BASE COUNT          245 a          181 c          217 g          290 t
ORIGIN      
        1 ccgcgctgat ggttcttatc cgttttattg aatcggagaa caaactgtga gctatcagcc
       61 tggtggtctg gttttgcctg atttaagccg ccgtgggtat ttgtccaaga atgcttcaag
      121 ctccaatcgt tttggcctgg tgaatggaga tgggttaagc aaagactctc gttgctggtg
      181 tactttttcg cgctggcgct caagcatgga taatgcgatt gcctgtagtt cttcgttagt
      241 cattgtctgc acctggaaag caatttaccg gagtacctca cactacggca aaaatgtttt
      301 attgttgcaa cttgcgtaat tattcaggtc agtttggaac gcactgtata aacataaata
      361 acctgatact gttgtgtgtt ttcaaagaaa acacaggtat ttttcacata aaccttcccc
      421 ttttcatatt cgaccactta atcaaaaaac ggttttatac ttgttgcccc cactggggtt
      481 aaatgtgaga catgtcgcac tttgtgccat gtgtcctcgc tgattttagg cgcggttcat
      541 tcattctttc tgttggcata aagtatggta tcggttgggc gaatgaaagg gactcgggtc
      601 atggtcagac aatacctgag gcgttaattc gttgaaagag attgtgaatg ggggaataac
      661 tgagtcttaa ctgctgtgcg atagcccgga cagatagttg ctcgtcgaat ctgagacgta
      721 atgattctgg caaataagtg cgaaagccgg tgatatctag tgttgtttga gagttcatcg
      781 ttcgcgtcca ttaatacaga tggacgcgat tgttgccaga caggataatt ttcacaagac
      841 atcgcagata gagtacttac gataattttt tattttatac aacagatgcc cacgaaaaga
      901 attattttca atagacacct gcattacaca aag
//
LOCUS       DAEEUE010000274          928 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.286, whole genome shotgun sequence.
ACCESSION   DAEEUE010000274 DAEEUE010000000
VERSION     DAEEUE010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.286"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            768..>928
                     /locus_tag="KLK84_004294"
     CDS             768..>928
                     /locus_tag="KLK84_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBE2011359.1"
                     /translation="MSQQPQQPQQPQQEISIINLDQLVSMTSVEIAELTGKEHRNVLR
                     DIRKMLEEL"
BASE COUNT          288 a          206 c          177 g          257 t
ORIGIN      
        1 gttttctcta cttatcaagg gataattaat acgactcact atagggagaa gggacacaaa
       61 aagttttctc ttctaggaca actaaccgta acttatcgca tcctttcagg ggcgggatga
      121 ccaattacag acaccaagcg ttctacttaa agtgaatgct tcctgtgtgc tctgggtcag
      181 tcgataagtg aatagcaata agacacaatc atcttaatta tggtctctag tccctacagg
      241 ggcttaactt aaagttaact taaagacaca attaaagatt aacttatagt attacttaaa
      301 gttaacttaa agacacaatt agagattaac tttaagtatt aaataaaaga tttaattaaa
      361 gaccaattaa agattaactc taagggaata ccttaagggg tgatgatatt tataataatc
      421 aatcaccaaa cctataggtc ttctctaggg tctctctata ggtctctcta gggtctcctc
      481 taggcattac ccccagaagc taactctatg gccctctatg gccctgtacg tcattcttta
      541 ggatgccctg tagtttcgta tagcctgatg cctagagaga ccctagagtg gctcctagag
      601 agaccctccc agtagctccg cacagctaca tcgtcatcat catagtattc aatctcaaag
      661 tcattctgtt tgcatcctgt tgtcctgctg gtctcccttc gcttggggac tttgagtagg
      721 tcagtggggg tgcgacttca gggtgcaaac aaaaaggaaa tcaagcgatg tctcaacaac
      781 cacaacaacc acaacaacca caacaagaaa tcagcatcat taacctcgac cagcttgtgt
      841 ccatgacctc agttgaaatt gctgagctga caggcaagga gcaccgaaat gtccttcgtg
      901 atattcgtaa gatgctcgaa gaacttaa
//
LOCUS       DAEEUE010000275          839 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.288, whole genome shotgun sequence.
ACCESSION   DAEEUE010000275 DAEEUE010000000
VERSION     DAEEUE010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.288"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            42..806
                     /locus_tag="KLK84_004295"
     CDS             42..806
                     /locus_tag="KLK84_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HBE2011360.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
BASE COUNT          178 a          237 c          276 g          148 t
ORIGIN      
        1 gaagcttgag catgcttggc ggagattgga cggacggaac gatgacggat ttcaagtggc
       61 gccatttcca gggtgatgtg atcctgtggg cggtgcgctg gtattgtcgc tatccgatca
      121 gctatcgcga ccttgaggaa atgctggcgg aacgcggcat ttcggtcgac catacgacga
      181 tctatcgctg ggtccagtgc tacgccccgg agatggagaa gcggctgcgc tggttctggc
      241 ggcgtggctt tgatccgagc tggcgcctgg atgaaaccta cgtcaaggtg cggggcaagt
      301 ggacctacct gtaccgggca gtcgacaagc ggggcgacac gatcgatttc tacctgtcgc
      361 cgacccgcag cgccaaggca gcgaagcggt tcctgggcaa ggccctgcga ggcctgaagc
      421 actgggaaaa gcctgccacg ctcaataccg acaaagcgcc gagctatggt gcagcgatca
      481 ccgaattgaa gcgcgaagga aagctggacc gggagacggc ccaccggcag gtgaagtatc
      541 tcaataacgt gatcgaggcc gatcacggaa agctcaagat actgatcaag ccggtgcgcg
      601 gtttcaaatc gatccccacg gcctatgcca cgatcaaggg attcgaagtc atgcgagccc
      661 tgcgcaaagg acaggctcgc ccctggtgcc tgcagcccgg catcaggggc gaggtgcgcc
      721 ttgtggagag agcttttggc attgggccct cggcgctgac ggaggccatg ggcatgctca
      781 accaccattt cgcagcagcc gcctgatcgg cgcagagcga cagcctacct ctgactgcc
//
LOCUS       DAEEUE010000276          833 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.289, whole genome shotgun sequence.
ACCESSION   DAEEUE010000276 DAEEUE010000000
VERSION     DAEEUE010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.289"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..326
                     /locus_tag="KLK84_004296"
     CDS             <1..326
                     /locus_tag="KLK84_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HBE2011361.1"
                     /translation="SETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGL
                     LLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVIL
                     KITYN"
     gene            complement(369..569)
                     /gene="glgS"
                     /locus_tag="KLK84_004297"
     CDS             complement(369..569)
                     /gene="glgS"
                     /locus_tag="KLK84_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HBE2011362.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          253 a          183 c          155 g          242 t
ORIGIN      
        1 ttagtgaaac gtttagtgta gtcatgacaa aagatcctgg tgcggcctgt actgagcaat
       61 tcaatatttt agggagcttt ttcactacgg atattttgag tgattatagc catctggata
      121 tgggtaatgg actgctattg aagatatttc ataaagatgg aacagcaacg gaatttaacc
      181 gcttctcaca atttgcttct ttttcatcgt ctagtgcacc ttcggtcacc gcaccattca
      241 gggcagaact tagtgcgaac ccggcagaga ctgttgttga gggaccgttt agtaaagacg
      301 taatcctcaa aatcacctat aactagcatc taatacaaac actaaaacgg gccatcaggc
      361 ccgttatatc agtgctctaa ctccagctct cttgcctgca tcatctgttg gcaataccag
      421 gcataacgtg cgcaaaacca ggttctatgt tcttcatcca tgttaccagt aacagccaga
      481 atatcgaccg ggcgaccttc tgcgtgcatt ctggcaaaac tacgcgccag gaaatcgaaa
      541 ttattcaaag aattaagact atgatccata agcacatcct ccggttatcg catacctttt
      601 aaggccatat gctttttctg atttcagtat tccccaatca gtgtagtgcc tttaaacata
      661 acgtgcgtaa atatattgtc cccgatcaca ctttttagtg aaaaataaaa aaccatatta
      721 aaatactttc tcttccagaa cttctctaac atgtgttatt attaacaaag acaaatgtaa
      781 gcagggaaac tattccaagg taaatattgc gtcactaaat ggacattgga gtg
//
LOCUS       DAEEUE010000277          812 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.290, whole genome shotgun sequence.
ACCESSION   DAEEUE010000277 DAEEUE010000000
VERSION     DAEEUE010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.290"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..366)
                     /locus_tag="KLK84_004298"
     CDS             complement(<1..366)
                     /locus_tag="KLK84_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE2011363.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGIFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEG"
     gene            complement(378..779)
                     /locus_tag="KLK84_004299"
     CDS             complement(378..779)
                     /locus_tag="KLK84_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HBE2011364.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
BASE COUNT          200 a          252 c          168 g          192 t
ORIGIN      
        1 cccttcataa tatcgtcctg cccttacgtt tcccggcagc ccctcacgcc gcaaccggat
       61 ataactacat ccgccatcat caggggtgat acgatccaca cagaaagact cacgaccaat
      121 catcagcgaa tcattgcgac acagccgtaa aatatctgct gttttcacaa acagggatgg
      181 taaagagtct tcaatacgca caccgccggc caccagagaa atattttcag gatcatcaaa
      241 aatcccggaa atcatgatcc cttttagccg gcctgacgta atgaccgctc taatccccat
      301 attgcgaata atggccttgt ctgcaagatc aagtgcaaca tcaaacagat tatcgaaatc
      361 agccacgctg cctcccgtta ttgcattctg gccaggccac gttctgtcat tccggctgcc
      421 acaccggcaa agacacggaa cgccattccc ggcggcacaa atgccagagg ttcatcccgc
      481 gtggcatgaa gtgcatcggt atgcagcgtc accagtgcca cgactgtaac cagatcatcc
      541 atattctgaa tcacggtctc cggctgcgct gatatcacct cattttcatg tccggtcagc
      601 gcattttccg ggtgggcaga cttatcccgg atgtcagtgt catccatatt ggcaagttcc
      661 tcctccagct cagccacacg ctgcgcaata tctgctgcgc ttcccgagat atccggctca
      721 cgtcccagcg atgctgccag ttctctcaga cgtttcaggt tatcgtcttt ctttgccata
      781 tctgttcctg tccgaataaa aaacgggagc cc
//
LOCUS       DAEEUE010000278          811 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.291, whole genome shotgun sequence.
ACCESSION   DAEEUE010000278 DAEEUE010000000
VERSION     DAEEUE010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.291"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..>811
                     /locus_tag="KLK84_004300"
     CDS             <1..>811
                     /locus_tag="KLK84_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HBE2011365.1"
                     /translation="KKLDVSCNKLTILTDLPENIKELIARDNFLTHISALPHYLITLD
                     VSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLKKLEVRNNELQTLPSLPSNLKI
                     LKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQENIIMRRFFYFENNQITTIPTNL
                     FRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQFRISLSDQNRLFLRQMLPQNL
                     HSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVSTQWTSFSTEENSRA"
BASE COUNT          259 a          187 c          122 g          243 t
ORIGIN      
        1 ttaaaaagct tgatgtgagc tgtaataaat taaccatcct tactgattta cctgaaaata
       61 ttaaagaact tattgcaaga gataatttct taacacatat atctgcatta ccacattatc
      121 taataacttt ggatgtgtcc gaaaatcaat tagagaatct gccgttatta ccagacacca
      181 tcaaatcact aagcgcagag tataataggt tatccacact gccttcatta cccttgaatt
      241 taaaaaaact tgaggttagg aacaacgaac tgcaaactct tccatctctg ccttctaatc
      301 ttaagatact taaggttgcg cacaaccatc ttactgaact gcccccttta cctaggagac
      361 tgcaacttct ttttgcatat agcaatagat taagcaactt accaaacatc caagaaaata
      421 ttatcatgag aagatttttt tattttgaaa acaaccaaat aactacaatc ccgacaaatc
      481 tttttcgttt agatcctcat ataactattg agattgcaaa taacccctta tcagatcaaa
      541 ctctgctatt cttaatacag caaacttcgg ttccaaattt taacgggcct cagtttcgta
      601 tttccctgtc agaccaaaac agactgtttt tacgccagat gttgccgcaa aatttacatt
      661 cgcgccatat cagagtcatc actgaagggg ggcagaactt tcagatcccc cctcttcccg
      721 aaactgtggc agcctggttt cctgaagcag atcgtcggga ggtttctaca caatggactt
      781 ctttttccac cgaggagaat tcccgggcat t
//
LOCUS       DAEEUE010000279          803 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.292, whole genome shotgun sequence.
ACCESSION   DAEEUE010000279 DAEEUE010000000
VERSION     DAEEUE010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.292"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..>803
                     /locus_tag="KLK84_004301"
     CDS             <1..>803
                     /locus_tag="KLK84_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBE2011366.1"
                     /translation="GVLVGYLPGLAMRRIADLHAGEAKTDARDAAIIAEAARTLPHAL
                     RTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRGLLTQIHPAPERVLGPRLEHPAV
                     LDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRLAADIAQALAEQTVVVPGTNAAA
                     VVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPVLTSMPGVGVRTAARLLTEVACR
                     AFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNKALKRALFLSAFAA"
BASE COUNT          157 a          273 c          215 g          158 t
ORIGIN      
        1 aaggagtcct tgtcggatac ctccctggac tggccatgcg ccgcatagcc gacttacacg
       61 ccggtgaagc taaaactgat gctcgtgacg ctgccatcat tgccgaagct gcccgtaccc
      121 tgcctcacgc gctacgcacg ctgaaactgg ctgacgagca aatcgccgaa ctctccatgc
      181 tctgcggctt cgatgatgat cttgccgcac agacaacgca ggccagcaac cgtatccgcg
      241 gccttctgac ccagatacat ccggcaccgg agcgcgttct cggtccgaga cttgagcacc
      301 cggcggtact cgatcttctc cagcgatatc cctcaccaga aaaactcgct tcgctgggtg
      361 agaagaagct ggcagcccag ctctgcaaac ttgcgcctcg tctgggtaaa cgccttgcag
      421 cagacatagc tcaggcactg gccgaacaaa ccgtcgtcgt tcccggcacg aatgccgctg
      481 ccgtagtact gccacgtctg gcactccagc tcatcacgct gcgtaagcaa agagacgagg
      541 tggcgcttga ggtagaacag cgagttcttg ctcaccctct ttacccggtc ctgaccagta
      601 tgcccggagt cggtgtcagg accgcagcca gactcctcac cgaggtcgcc tgccgcgcct
      661 tcgcctctgc cgcacatctc gctgcttatg ctggccttgc gccggtaact cggcgatccg
      721 gctcgtcaat acgcggtgag catccctcgc gacggggtaa taaagctctc aaacgggcgt
      781 tgttcctgtc ggccttcgcc gcg
//
LOCUS       DAEEUE010000280          789 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.293, whole genome shotgun sequence.
ACCESSION   DAEEUE010000280 DAEEUE010000000
VERSION     DAEEUE010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.293"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            1..789
                     /locus_tag="KLK84_004302"
                     /pseudo
     CDS             1..789
                     /locus_tag="KLK84_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
BASE COUNT          241 a          145 c          174 g          229 t
ORIGIN      
        1 aataaacaaa tcaccgctaa aggtggctgg aaagctattt cagaaagtac acaggcacaa
       61 ctagggaact tgaaaaactc ttggaatacc acgcttgata gtatcttcag aggttctgaa
      121 aatggtctgc aagattttac aagaagctta accaatcttt taaacgctct tggtggaagt
      181 ggtaagagtc ttggtgaatc tcttggtagt ctcatgactt ctatgtcaca tggtgtcgat
      241 tcattgaccg atattagtta cagggtaagg gcgttctttg atgaggtgac attagcttac
      301 cgtccactaa atgacactca gaggaaaatt gttgatggtt tatctggtgg gttaattgat
      361 ggtctgaaga tgcttgctgt ggctgttact gctcaaaagg ctgcaaatgt agcaggaggc
      421 ttgttaaacc ttgcaaacgc tgttacttca ttcggtgacg ctgttaataa agctaaagct
      481 ggcaaggctg gttcttcagg tggtgcgcta aataaagtcg gtagagttgt cctttatgct
      541 gagttggtaa attttgctct tgataattta aaccctgaag cagctaagaa tccgaactta
      601 catcaatcag gtattggcac agaaacaaac ttcaaccctg aatacaaagg tgattcatct
      661 aagccaatag ttctatcgct gactggttta atggtgcaac taataaaatt gctaacttcc
      721 aagaaccgat gaataacttc gtaaataaca cacaatcaac tccatcagct atcacaggtg
      781 acatggtta
//
LOCUS       DAEEUE010000281          785 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.294, whole genome shotgun sequence.
ACCESSION   DAEEUE010000281 DAEEUE010000000
VERSION     DAEEUE010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.294"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..297
                     /locus_tag="KLK84_004303"
     CDS             <1..297
                     /locus_tag="KLK84_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HBE2011367.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..569)
                     /locus_tag="KLK84_004304"
                     /pseudo
     CDS             complement(405..569)
                     /locus_tag="KLK84_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
     gene            616..>785
                     /locus_tag="KLK84_004305"
     CDS             616..>785
                     /locus_tag="KLK84_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE2011368.1"
                     /translation="MIVLILVFRLVIGEQMIDVLGPEKRRRRTTQEKIAIVQQSFEPG
                     MTVSLVARQHGVA"
BASE COUNT          246 a          182 c          159 g          198 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgtagcc ttgtaatgat agactggccc cctgaatctc cagacaacca
      601 gtatcactta tttaagtgat agtcttaata ctagttttta gactagtcat tggagaacag
      661 atgattgatg tcttagggcc ggagaaacgc agacggcgta ccacacagga aaagatcgca
      721 attgttcagc agagctttga accggggatg acggtctccc tcgttgcccg gcaacatggt
      781 gtagc
//
LOCUS       DAEEUE010000282          780 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.295, whole genome shotgun sequence.
ACCESSION   DAEEUE010000282 DAEEUE010000000
VERSION     DAEEUE010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.295"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(275..772)
                     /locus_tag="KLK84_004306"
     CDS             complement(275..772)
                     /locus_tag="KLK84_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HBE2011369.1"
                     /translation="MTTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGNDLKE
                     LRVALNYSQNPVSPKARSLILWTERGAARHGKMLETDQAWDVFEKLEDCYFSQRDPST
                     PVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDKLVE
                     RLMQL"
BASE COUNT          208 a          196 c          174 g          202 t
ORIGIN      
        1 gctggacttg aaccagcgac ctggcgatta tgagtcgctc gctctgacca actgagctaa
       61 agggccggag gcagaataat aaccatatgt catcacatct gcaaactcat ctgaccacca
      121 gcgcgtttaa cgccctgtgc catttttcag gcacaaaaaa acccgcataa agcgggttct
      181 ttcaagtgtc catgtctgct attcgcctcg cgatacagct ttgcgaagct taccggaatt
      241 gaagcagttt atgtgcaaaa atgcaagaac ttttttaaag ctgcatcaac ctttccacca
      301 gtttatctct gcgaacaaca aaccaaccat tggctctcgc cagttccagc catgactcaa
      361 gggaaataac aatatcatca tcccgtaact gaattgtgga aacagtgaca ccgcctctct
      421 gataacagag aactcgcgtg tcgtaacttt tctggcatga aactggcgtt gacggatccc
      481 tttgactgaa atagcagtct tccagttttt cgaacacatc ccacgcctga tcggtttcga
      541 gcattttgcc gtggcgtgct gcgcctcgtt ctgtccagag gatgagggag cgggctttgg
      601 gtgaaactgg attttgtgag tagtttaaag cgacccgcaa ttctttaagg tcattaccaa
      661 caactttgaa aaagtgtttc ccttcaacga agcgtacttt gttctcatga tgattctggc
      721 gaatacgcac cggctcagtg ccgtaaagct gcgccaaaag ttcggtggta ataacaggaa
//
LOCUS       DAEEUE010000283          755 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.296, whole genome shotgun sequence.
ACCESSION   DAEEUE010000283 DAEEUE010000000
VERSION     DAEEUE010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.296"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(137..754)
                     /locus_tag="KLK84_004307"
     CDS             complement(137..754)
                     /locus_tag="KLK84_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HBE2011370.1"
                     /translation="MSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFDDR
                     DQAVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAWRT
                     EQEAKPFTFEWNGRTWNADASSVARLSPVVMLAKSVAAQTHMVWSDADNQQVKLSMPE
                     LEELAAAMVQAQVDRNDEIYRRQREMKEELSSLDDLASIRAFDVK"
BASE COUNT          146 a          239 c          179 g          191 t
ORIGIN      
        1 gaggcccgcc cccgggcctc cccggattca tccgtttctc tgttcagcct gaccgcacac
       61 cccggcggcc ggatgacaga ctccgcctcg gtaagcaaag cggtcttctg tgattccgcc
      121 agttgcggct tattcattac ttaacgtcaa acgcccgaat tgaagccaaa tcatccagac
      181 tgctcagctc ctctttcatc tcccgctgac ggcgataaat ctcatcgttg cgatcgacct
      241 gcgcctgcac cattgctgcc gccagttctt ccagttccgg catcgacagt ttcacctgct
      301 gattatccgc atcactccac accatatgtg tttgtgccgc gacagatttt gccagcatga
      361 ccaccgggga caggcgggca actgacgagg cgtcagcgtt ccacgtgcgg ccgttccatt
      421 caaacgtgaa cggcttcgcc tcctgttctg tgcgccatgc ttcaatttcc tgacgtctgg
      481 cctctctggc cgcttccagc atttctggtg tcacagtgaa tggggctatc tcaccccatt
      541 tgccactttg cagttcctgc cagatttgct gacccgtcgg tgcaacatca tcagcggtgg
      601 ctgtgtaggg aactgcctgg tccctgtcgt caaaaaaaac gtcacagtct actgcgccac
      661 tttcggtata acggggatta atgatttttt taatttccac ggtgcattcc tcacgatgtg
      721 cgaataaaaa gcccgggcat tgcgccagag acatg
//
LOCUS       DAEEUE010000284          747 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.297, whole genome shotgun sequence.
ACCESSION   DAEEUE010000284 DAEEUE010000000
VERSION     DAEEUE010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.297"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..>747
                     /locus_tag="KLK84_004308"
     CDS             <1..>747
                     /locus_tag="KLK84_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HBE2011371.1"
                     /translation="DVLLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYR
                     LMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDC
                     CDREALHWAVTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQ
                     FARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYN
                     EWHPHSALGYRSPREYLRQRACNGLSDNRCL"
BASE COUNT          180 a          177 c          232 g          158 t
ORIGIN      
        1 ggatgtgctt ctccgtatac accatgttat cggagagctg cccacgtatg gttatcgtcg
       61 ggtatgggcg ctgcttcgca gacaggcaga acttgatggt atgcctgcga tcaatgccaa
      121 acgtgtttac cggctcatgc gccagaatgc gctgttgctt gagcgaaaac ctgctgtacc
      181 gccatcgaaa cgggcacata caggcagagt ggccgtgaaa gaaagcaatc agcgatggtg
      241 ctctgacggg ttcgagttct gctgtgataa cggagagaga ctgcgtgtca cgttcgcgct
      301 ggactgctgt gatcgtgagg cactgcactg ggcggtgact accggcggct tcaacagtga
      361 aacagtacag gacgtcatgc tgggagcggt ggaacgccgc ttcggcaacg atcttccgtc
      421 gtctccagtg gagtggctga cggataatgg ttcatgctac cgggctaatg aaacacgcca
      481 gttcgcccgg atgttgggac ttgaaccgaa gaacacggcg gtgcggagtc cggagagtaa
      541 cggaatagca gagagcttcg tgaaaacgat aaagcgtgac tacatcagta tcatgcccaa
      601 accagacggg ttaacggcag caaagaacct tgcagaggcg ttcgagcatt ataacgaatg
      661 gcatccgcat agtgcgctgg gttatcgctc gccacgggaa tatctgcggc agcgggcttg
      721 taatgggtta agtgataaca gatgtct
//
LOCUS       DAEEUE010000285          714 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.298, whole genome shotgun sequence.
ACCESSION   DAEEUE010000285 DAEEUE010000000
VERSION     DAEEUE010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.298"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            60..400
                     /locus_tag="KLK84_004309"
                     /pseudo
     CDS             60..400
                     /locus_tag="KLK84_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            475..>714
                     /locus_tag="KLK84_004310"
     CDS             475..>714
                     /locus_tag="KLK84_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE2011372.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWATICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTTAERQRLKELEREN"
BASE COUNT          219 a          161 c          185 g          149 t
ORIGIN      
        1 ttgttctgag gtgagcatat cacctctgtt caggtggcca aattcagtgt gccacttcaa
       61 acagtgaatt atttgccaac gacggtacac cgacatggct aaaagaaaag cgtgcaaaag
      121 aaatcgcact tgatgaaatt aacttgatga aattatcgag ttgctcaaca agcaccatgg
      181 gggcaccagt aacgacgcca aacgcgcaaa agctgatttg ctggaaaaaa cattcacctc
      241 ccgctcatgg gaaagaatca aaggtatgga ctggccgaca atcaaagctg gtcgtaatgc
      301 actatggatt gaactggaag gcgttgaata tgcattcccg gatccacaaa cacaaaacga
      361 aacgcaacaa gcaggatacg atgagaatat tccggtgtaa aaccaagtgc attttgggaa
      421 tgaaccgccc cgggaatcct ggagactaaa cttcctgaga aagaggtaaa caggatgact
      481 aaaaatactc gtttttcccc cgaagtccgt caacgggcag tccgtatggt tctggaaagt
      541 cagggcgaat atgactcaca atgggcgaca atttgttcca ttgccccaaa gattggctgt
      601 acgccggaga ctctgcgtgt ctgggttcgc cagcatgagc gggataccgg gggcggtgat
      661 ggagggctca ccaccgctga acgtcagcgt ctgaaagagc tggagcgtga aaat
//
LOCUS       DAEEUE010000286          701 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.299, whole genome shotgun sequence.
ACCESSION   DAEEUE010000286 DAEEUE010000000
VERSION     DAEEUE010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.299"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            62..607
                     /locus_tag="KLK84_004311"
                     /pseudo
     CDS             62..607
                     /locus_tag="KLK84_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          175 a          176 c          179 g          171 t
ORIGIN      
        1 acgaatcgga aaatcgatac tggaaaaact ctaactcggt ggccagtttt tgttgaccac
       61 ttcaggtcag gagattgtcg tcgaaccgat tgaacaggct attctcatct ctgagcagac
      121 taagctgtgg gacgccaagt catccaccgg attcactcag ggcacacttc agcaggacgc
      181 tatgaacatc tgtgagaatg tggtccgtac catcaacgct cgcatggtcg acgttctgga
      241 atcctctaag ctgctgaaga ccgtagagct tcctgtcctg actggtagcc tgaccgctaa
      301 ggcagacgct atcatggacg ctctgtatga gaacaccgag ccttctttcg gttctgaggt
      361 gtctgactac ggtattatcg cccatgagag ccaactgaaa gcactgtccc gtctggctgc
      421 taaacagggc ttctctggtg aagacgctat cgtggatatg ctgggtaccg acatcgcgta
      481 ttacaacggt gaggacaaag gtgtgttcat gttggctaaa cgcttcacgg ccctgagctt
      541 cggctgcttc cgtcacgacg gtgagaatat tacggtcgtc ctttcacgtg atggcgactc
      601 tcagtcctga ggtagcctga gtttaacgga cactccttcc tgaaatagaa tggcatcaga
      661 aggagctaat aatgagcaga aaaacccaac gttactctaa a
//
LOCUS       DAEEUE010000287          695 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.300, whole genome shotgun sequence.
ACCESSION   DAEEUE010000287 DAEEUE010000000
VERSION     DAEEUE010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.300"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            1..525
                     /locus_tag="KLK84_004312"
                     /pseudo
     CDS             1..525
                     /locus_tag="KLK84_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            589..>695
                     /locus_tag="KLK84_004313"
     CDS             589..>695
                     /locus_tag="KLK84_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBE2011373.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDITT"
BASE COUNT          194 a          144 c          166 g          191 t
ORIGIN      
        1 tgtgctgatc gtgcggatga ttactaccat gaccgtctgg cgctgactgt tgataattca
       61 acgatcgacg acaactacga acactacaca tataacggca cttacaataa tgccgctgat
      121 actcatgttg tagacgttta caacattggt acggctatta ctctggatca ggaagttgat
      181 ctgtccatca ctaataactc tcacgttgcg ggtattacgt tgactcaggg ttatgagtgg
      241 gaagatattg acgacaacac tgtcagcact ggcgtaaaca gcagcgaagt gtttaataac
      301 accatcactg ttaaagattc taccgtaacc tctggttcat ggactgatga aggtactact
      361 ggttggttcg gtaatacagg taatgccagc gattatagtg gtaaatctaa ctttgtcacc
      421 gtagatactg acggagatgg tgttgctgat agcactattg caagctggga tgatgttgca
      481 ttagctgtag ttgcacatcc gaatgttgat aatgctatgc agacctgaat cgccacgggt
      541 ttaacagaca cctcagagtc atttaagatg gcttaaagag aggtgcccat gagcggtaag
      601 cgttatcccg aagagtttaa aactgaagca gtcaaacagg ttgttgatcg cggttattct
      661 gttgccagcg ttgcaacacg tctcgatatc accac
//
LOCUS       DAEEUE010000288          687 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.301, whole genome shotgun sequence.
ACCESSION   DAEEUE010000288 DAEEUE010000000
VERSION     DAEEUE010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.301"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..667)
                     /locus_tag="KLK84_004314"
     CDS             complement(<1..667)
                     /locus_tag="KLK84_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBE2011374.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          185 a          173 c          154 g          175 t
ORIGIN      
        1 ttgccgcatc atcatcagac tccggaacgc cctcatacac atgtttaaaa ccatccggat
       61 cacggcgtcg cgccgcgtcg ataaccttaa gcatggtgtc agacagaaac ggattttcgt
      121 catagttaat tttgcggatg agagtgcctt cgggcggatc aacaacgaag ttgcgccaga
      181 cgaaatcagt aacaagtccg gggttgaata tgaaccagca ttccgaaccc tctttacgga
      241 tcgttggctc cagaattttc cactggtatt ccgtcagtgc gtgggcttct tccagccaca
      301 acatatcgat cccctccagt gacttaattt cttcgatgtt gcgccataat ccataaaaaa
      361 caaattccga gccagtaacc cggttaatga ttttgttgtt cagaatacgg aaacggtgcc
      421 gcagaccaaa cctgtctatc tgaattttga gcagggtata caccgactct tcgattttat
      481 tctggatctg acgggcacaa caaaaacgca gggtgtattt attcgacaga aatatggcaa
      541 tgccagcggc atcccatgat tttgacgatg accgaccacc ataaagcact ttgttacgtg
      601 cccgcatagt ccagaaacta cgtaaagccg gattcagcgt cggtttggat gtcagagtag
      661 aagtcattga ggtcacgctc tccattg
//
LOCUS       DAEEUE010000289          685 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.302, whole genome shotgun sequence.
ACCESSION   DAEEUE010000289 DAEEUE010000000
VERSION     DAEEUE010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.302"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..>685
                     /locus_tag="KLK84_004315"
     CDS             <1..>685
                     /locus_tag="KLK84_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBE2011375.1"
                     /translation="ITLADNFKQKNEALDTWYVGMNDFARIAGGQNSRSNILSPRAFL
                     EFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKFYMNNPEGERADL
                     SKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDDSSLHLITNKVLA
                     CYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNNSQVSCKGSVDST
                     ITDLLEKALN"
BASE COUNT          239 a          100 c          147 g          199 t
ORIGIN      
        1 cattacatta gccgacaatt ttaaacaaaa aaatgaagcc ttagatacct ggtatgtggg
       61 aatgaatgat tttgcccgaa ttgccggggg gcagaatagc agaagcaata ttctttctcc
      121 tagagcattt ttggagtttt tggcaaagat atttaccctg ggttatgtgg attttagcaa
      181 acgctccaac gaagcgggta gaaatatgat ggctcatatt gagttctcat cttattctaa
      241 agatactgat ggcaatgaaa aaatgaagtt ttacatgaat aatcctgaag gggagcgagc
      301 ggatttatct aaggtgaaga tagaaattac acttgcctca gcttctacta aagggattcg
      361 tgaaggacat acagtcatta tatttaaaca atctgatggt tcgactaatc gttatgaagg
      421 gaagtctttt gaaagaaaag atgatagttc attacacctg attactaaca aggttctggc
      481 gtgttaccaa cgcgaagcta acaagaaaat agcgcgtcta ttaaataatc atcagaagtt
      541 aaataatata caggagttaa atgattcgca ggagttaaat aattcacaga agttaaataa
      601 ttcgcaggag ttaaataact cgcaggtgag ttgtaaaggt tcagttgatt ctacgattac
      661 ggatttatta gaaaaagcat tgaat
//
LOCUS       DAEEUE010000290          665 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.303, whole genome shotgun sequence.
ACCESSION   DAEEUE010000290 DAEEUE010000000
VERSION     DAEEUE010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.303"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            1..665
                     /locus_tag="KLK84_004316"
                     /pseudo
     CDS             1..665
                     /locus_tag="KLK84_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          170 a          170 c          180 g          145 t
ORIGIN      
        1 ataaagaagt acggtccgga ttcttccact aataaagaac agtcagatgc tcaggccgag
       61 atccgccgtc tccagaaaga gctgaaacgg gttaccgacg aacgggacat attaaaaaaa
      121 gccgcggcgt acttcgcaaa gctgtccgac tgaggtacgc ctttatccgt gacaactcct
      181 gttgctggcc tgttcgcctg ctctgtcggg tgctggatgt tcatcccagt ggtttttacg
      241 cctggcttca gcagccgcat tcacaacgcc atcaggcaga cctgagactg acaggacaga
      301 ttaaacagtt ctggctggaa tcgggatgcg tctatggtta tcgcaaaatc catctggatc
      361 tgcgtgacag cgggcaacag tgcggagtaa acagagtctg gagactgatg aaacgtgtcg
      421 gaataaaggc tcaggtcgga taccgaagcc cgcgggcacg taaaggcgag gccagtatcg
      481 tgtcacccaa caggctccag cgacagttca atccggatgc tcctgatgag cgttgggtaa
      541 cggacataac ctacatcagg acccacgaag gctggctgta tcttgccgtt gttgttgatc
      601 tgttctcacg caaaattatc ggctggtcca tgcaatcccg gatgacaaag gacattgtcc
      661 tgaac
//
LOCUS       DAEEUE010000291          656 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.304, whole genome shotgun sequence.
ACCESSION   DAEEUE010000291 DAEEUE010000000
VERSION     DAEEUE010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.304"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..491
                     /locus_tag="KLK84_004317"
     CDS             <1..491
                     /locus_tag="KLK84_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE2011376.1"
                     /translation="LGALWVRNGMEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFL
                     KIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLAD
                     RFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFT
                     EM"
     gene            553..>656
                     /locus_tag="KLK84_004318"
     CDS             553..>656
                     /locus_tag="KLK84_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE2011377.1"
                     /translation="MSRKTRRYSKEFKAEAVRTVLENQLSISEDASRL"
BASE COUNT          166 a          142 c          161 g          187 t
ORIGIN      
        1 tactgggagc attgtgggtg cgcaatggca tggaggagtc cgcggaattt gaacaacagc
       61 aacataatca agcggccgcg aaaaaacgca tcccggttat cgaagctctg ttacgacatc
      121 ccggtgcttt cctgaagatt attgcactac gactgtgcga gttgctgacg atgtacattg
      181 ttactgcctt tgcacttaat tattcaaccc agaatatggg gttaccgcgc gaacttttcc
      241 ttaatattgg tttgctggta ggtggattaa gctgcctgac aattccctgt tttgcctggc
      301 ttgccgatcg ttttggtcgg cgcagggttt atatcacagg cgcgttgatc ggaacgttga
      361 gcgcatttcc tttctttatg gcgcttgaag cacaatctat tttctggata gttttcttct
      421 ccataatgct ggcaaacatt gcgcatgaca tggtggtgtg tgtgcaacaa ccgatgttta
      481 ccgaaatgtg aggtagcctg agtttaacgg acactccttc ctgaaataga atggcatcag
      541 aaggagctaa taatgagcag aaaaacccga cgttactcta aagagttcaa agccgaagct
      601 gtcagaacgg ttcttgaaaa tcaactttcg atcagtgaag acgcttcccg attatt
//
LOCUS       DAEEUE010000292          630 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.305, whole genome shotgun sequence.
ACCESSION   DAEEUE010000292 DAEEUE010000000
VERSION     DAEEUE010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.305"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..112)
                     /locus_tag="KLK84_004319"
     CDS             complement(<1..112)
                     /locus_tag="KLK84_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HBE2011378.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYI"
     gene            complement(109..399)
                     /locus_tag="KLK84_004320"
     CDS             complement(109..399)
                     /locus_tag="KLK84_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HBE2011379.1"
                     /translation="MVDLLKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(399..>630)
                     /locus_tag="KLK84_004321"
     CDS             complement(399..>630)
                     /locus_tag="KLK84_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HBE2011380.1"
                     /translation="FREWVAIQAGFYTEHFYPDGSRGCRAKSIAFANMDETEFQQVYK
                     SVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
BASE COUNT          149 a          181 c          136 g          164 t
ORIGIN      
        1 caatatacga ccagcgcccc cacatacgta gttttccctg aatccagaca ctttccagcg
       61 tggtgagacg aaggtgttct ccgctttttc ctgtattcgt tgggtaaatc ataaatatcc
      121 ctcctttctc cagatttctt gtgtgcgaaa aacaccttct gcatgcatca ggcgtaattc
      181 ttctttggtg taatcgctgg tttttacccg cccgtcgatt aaatcgtggc acgagctaca
      241 ggcaatcgct gcctgcatat cgtgtggctt tatcgctgtt ccgcacgttc ccgccagtcg
      301 gtaatgcgcc agcacagacg tttccggatc gtgattgcag tagccaggaa ttctgacggt
      361 gcacatctgc ccccgcgccg ctttaagtaa atccaccatt acgcaaactc cagcagctgc
      421 gcggccacat tttcgacttg ttccggagag gaaaatttac ggaacagaat ccagttccac
      481 agcacattca gtacagattt ataaacctgc tgaaactcgg tttcgtccat attcgcaaac
      541 gcgatagatt tcgcccgaca cccacggcta ccgtccggat aaaaatgctc ggtgtaaaat
      601 ccggcctgaa tggctaccca ctcgcggaaa
//
LOCUS       DAEEUE010000293          628 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.306, whole genome shotgun sequence.
ACCESSION   DAEEUE010000293 DAEEUE010000000
VERSION     DAEEUE010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.306"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..131)
                     /gene="csgC"
                     /locus_tag="KLK84_004322"
     CDS             complement(<1..131)
                     /gene="csgC"
                     /locus_tag="KLK84_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HBE2011381.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQI"
     gene            complement(190..555)
                     /gene="csgA"
                     /locus_tag="KLK84_004323"
     CDS             complement(190..555)
                     /gene="csgA"
                     /locus_tag="KLK84_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HBE2011382.1"
                     /translation="MPVHLSNNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHG
                     GGNGADVGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASN
                     SSVNVTQVGFGNNATAHQY"
BASE COUNT          164 a          142 c          150 g          172 t
ORIGIN      
        1 aatatttgta ctctgcacag acaagattga gtaagagtaa cttcaggaat aatggtgtac
       61 atatcccctt gctgggtcgt attaaaggtt atctggctgg aaagtgccgc aaggagtaat
      121 aacgcattca tattcttctc ccgaaaaaaa acagggcttg cgccctgttt ctgtaataca
      181 aatgatgtat taatactgat gagcggtcgc gttgttacca aagccaacct gagtcacgtt
      241 gacggaggag ttcgatgcag tctggtcaac tgcagcaccg ttgccaccac cgaactgttt
      301 aacagtcatt tcagaatttt tgccgttcca ctgatcaaga gtggcgctgt taccgaagcc
      361 acgttgggtc agatcgattg agctgtcatc tgagccctga ccaacatctg caccattacc
      421 accgccatgc tgggtaatag tcaagtcaga gttacgggca tcagtttgca gagcaagtgc
      481 agagttaccg ccaccgtact ggtaaatgtt cagctctgaa tttgggccgc tattattact
      541 gaggtgtact ggcaatagcg gacactacca tttgttcttt tttaagcagc catctgatga
      601 tatttttccc tgaaggctgc cggggaga
//
LOCUS       DAEEUE010000294          624 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.307, whole genome shotgun sequence.
ACCESSION   DAEEUE010000294 DAEEUE010000000
VERSION     DAEEUE010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.307"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(1..>624)
                     /locus_tag="KLK84_004324"
     rRNA            complement(1..>624)
                     /locus_tag="KLK84_004324"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          133 a          188 c          149 g          154 t
ORIGIN      
        1 aggaggtgat ccaaccgcag gttcccctac ggttaccttg ttacgacttc accccagtca
       61 tgaatcacaa agtggtaagc gccctcccga aggttaagct acctacttct tttgcaaccc
      121 actcccatgg tgtgacgggc ggtgtgtaca aggcccggga acgtattcac cgtggcattc
      181 tgatccacga ttactagcga ttccgacttc atggagtcga gttgcagact ccaatccgga
      241 ctacgacgca ctttatgagg tccgcttgct ctcgcgaggt cgcttctctt tgtatgcgcc
      301 attgtagcac gtgtgtagcc ctggtcgtaa gggccatgat gacttgacgt catccccacc
      361 ttcctccagt ttatcactgg cagtctcctt tgagttcccg gccggaccgc tggcaacaaa
      421 ggataagggt tgcgctcgtt gcgggactta acccaacatt tcacaacacg agctgacgac
      481 agccatgcag cacctgtctc acggttcccg aaggcacatt ctcatctctg aaaacttccg
      541 tggatgtcaa gaccaggtaa ggttcttcgc gttgcatcga attaaaccac atgctccacc
      601 gcttgtgcgg gcccccgtca attc
//
LOCUS       DAEEUE010000295          601 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.308, whole genome shotgun sequence.
ACCESSION   DAEEUE010000295 DAEEUE010000000
VERSION     DAEEUE010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.308"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..127
                     /locus_tag="KLK84_004325"
     CDS             <1..127
                     /locus_tag="KLK84_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HBE2011383.1"
                     /translation="VTCKQNEVEPEDWLREVIEKLNDWPSNQVHELLPWNFSSVK"
     gene            complement(161..364)
                     /locus_tag="KLK84_004326"
                     /pseudo
     CDS             complement(161..364)
                     /locus_tag="KLK84_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
     gene            464..>601
                     /locus_tag="KLK84_004327"
     CDS             464..>601
                     /locus_tag="KLK84_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE2011384.1"
                     /translation="MRMNMSDFATSFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LK"
BASE COUNT          156 a          146 c          137 g          162 t
ORIGIN      
        1 ggtcacctgc aaacagaacg aagtggagcc ggaggactgg ttgcgcgaag tgatcgagaa
       61 gctcaatgac tggccgtcga accaagtgca tgaactgctg ccctggaact tctcgtctgt
      121 aaaataatcc ttacgctacg tacttctcgg ggcgcttacc tggagaataa cgtttatcca
      181 tattcttcca gtctggttca tcttccgacg tcatcaccgg gttgaaatta cgccgatata
      241 aatcgagttc cgtcacctgt attttccgct ccattgcccg ctgatggatc gcatcagcaa
      301 tatttgcggt caatgaatca tgacgaggat gggcccatac caaataaata cgttcagact
      361 gcataatttt acctccacgt taagttctgg agcgcacttt acgcaggcgg cataatcctg
      421 taaacgtgat gattcaggat gagttaatga aggattttca ctaatgcgga tgaatatgtc
      481 tgactttgcc acttcctttg ccgtggcccg taatcaaagc tttcgtgcag caggcgatga
      541 gttaggctta tcctcgtccg ccattagcca tagtattaaa acactggaac aacgtcttaa
      601 a
//
LOCUS       DAEEUE010000296          587 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.309, whole genome shotgun sequence.
ACCESSION   DAEEUE010000296 DAEEUE010000000
VERSION     DAEEUE010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.309"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..290)
                     /locus_tag="KLK84_004328"
     CDS             complement(<1..290)
                     /locus_tag="KLK84_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HBE2011385.1"
                     /translation="MSPSLRKAVAVAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLVTVARQINPYIKVDIPET"
     gene            complement(290..418)
                     /locus_tag="KLK84_004329"
                     /pseudo
     CDS             complement(290..418)
                     /locus_tag="KLK84_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
     gene            483..>587
                     /locus_tag="KLK84_004330"
     CDS             483..>587
                     /locus_tag="KLK84_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE2011386.1"
                     /translation="MSRKTQRYSKEFKAEAVRTVLENQLSISEGASRLS"
BASE COUNT          160 a          145 c          124 g          158 t
ORIGIN      
        1 gtttccggta tatcgacttt gatgtacggg ttaatttgtc tggcgacagt gacaaggtct
       61 ttattcagga gagctttgca ttctgcttcg gtatacgttt taccaggcat gatgtctttt
      121 ccggtgtgtc cgtgacatac agtccataca ccaatgatat ctttgtatgg tatgtagctg
      181 acaccttcca gaccatcgtt accacctggg ccagtgatta acacagatgc tatagcaaca
      241 gccccgccac caatagcaac tgcaacagcc ttgcgtaatg atggcgacat tattcacctc
      301 tcgcagcctt acgcttatct tctcttattt tgaaatacag attcgtcaga taagtcagaa
      361 gccccagaag cagacttccc agcacaccaa tcgcagccca ctgtgatgga ctgacctgtg
      421 aggtagcctg agtttaacgg acactccttc ctgaaataga atggcatcag aaggagctaa
      481 taatgagcag aaaaacccaa cgttactcta aagagttcaa agccgaagct gtcagaacgg
      541 ttcttgaaaa tcaactttcg atcagtgaag gcgcttcccg attatct
//
LOCUS       DAEEUE010000297          586 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.310, whole genome shotgun sequence.
ACCESSION   DAEEUE010000297 DAEEUE010000000
VERSION     DAEEUE010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.310"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..>586
                     /locus_tag="KLK84_004331"
     CDS             <1..>586
                     /locus_tag="KLK84_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HBE2011387.1"
                     /translation="LAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAGT
                     DLFMTNRTNGVATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTID
                     GFSFAGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGD
                     VVNKTQNFEAVLQYQFDFGLRPSIGYSYSKALDVA"
BASE COUNT          153 a          114 c          145 g          174 t
ORIGIN      
        1 gtttggcata tgctggtttg agttacaaag attttggttc attcgactac agtcgtaatg
       61 tcggtgtcgc gtatgatgca gaagcattta ctgatatgtt tgttgagtgg ggcggtgact
      121 catgggctgg tactgacctg ttcatgacca accgtaccaa cggtgttgca acctatcgta
      181 acacagattt ctttggcatg gttgaaggtc taaattttgc tctgcaatat cagggtaaaa
      241 atgaaggtac aggcaactac aaagcaaatg gcgatggcca tggtttatct gcaacttaca
      301 ctattgacgg tttctcattt gctggtgctt atgcaaactc tgaccgtact gattggcagt
      361 ctggtgatgg taaaggcgaa agagccgaag tttgggcgtt gtcaacaaaa tatgacgcta
      421 acaatgttta tgctgctgtg atgtacggtg aatctcacaa catgaattcc gacgacggtg
      481 atgttgtgaa caaaacacaa aacttcgaag ctgttctgca atatcagttc gactttggtc
      541 tgcgtccatc catcggttac tcctattcta aagcactgga cgtagc
//
LOCUS       DAEEUE010000298          573 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.311, whole genome shotgun sequence.
ACCESSION   DAEEUE010000298 DAEEUE010000000
VERSION     DAEEUE010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.311"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..>573
                     /locus_tag="KLK84_004332"
     CDS             <1..>573
                     /locus_tag="KLK84_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HBE2011388.1"
                     /translation="NPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVD
                     FYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVE
                     HRQIKYRNNVIECDHGKLKRIIGATLGFKS"
BASE COUNT          137 a          155 c          156 g          125 t
ORIGIN      
        1 tgaacccatt caaaggccgg cattttcagc gtgacatcat tctgtgggcc gtacgctggt
       61 actgcaaata cggcatcagt taccgtgagc tgcaggagat gctggctgaa cgcggagtga
      121 atgtcgatca ctccacgatt taccgctggg ttcagcgtta tgcgcctgaa atggaaaaac
      181 ggctgcgctg gtactggcgt aacccttccg atctttgccc gtggcacatg gatgaaacct
      241 acgtgaaggt caatggccgc tgggcgtatc tgtaccgggc cgtcgacagc cggggccgca
      301 ctgtcgattt ttatctctcc tcccgtcgta acagcaaagc tgcataccgg tttctgggta
      361 aaatcctcaa caacgtgaag aagtggcaga tcccgcgatt catcaacacg gataaagcgc
      421 ccgcctatgg tcgcgcgctt gctctgctca aacgcgaagg ccggtgcccg tctgacgttg
      481 aacaccgaca gattaagtac cggaacaacg tgattgaatg cgatcatggc aaactgaaac
      541 ggataatcgg cgccacgctg ggatttaaat cca
//
LOCUS       DAEEUE010000299          535 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.312, whole genome shotgun sequence.
ACCESSION   DAEEUE010000299 DAEEUE010000000
VERSION     DAEEUE010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.312"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..>535)
                     /gene="intI1"
                     /locus_tag="KLK84_004333"
     CDS             complement(<1..>535)
                     /gene="intI1"
                     /locus_tag="KLK84_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HBE2011389.1"
                     /translation="VHWVRAFIRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQ
                     ALAALLFFYGKVLCTDLPWLQEIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQL
                     LYGTGMRISEGLQLRVKDLDFDHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARA
                     WWLKDQAEGRSGVALPDA"
BASE COUNT          116 a          183 c          153 g           83 t
ORIGIN      
        1 gggcgtcggg aagcgcaacg ccgctgcggc cctcggcctg gtccttcagc caccatgccc
       61 gtgcacgcga cagctgctcg cgcaggctgg gtgccaagct ctcgggtaac atcaaggccc
      121 gatccttgga gcccttgccc tcccgcacga tgatcgtgcc gtgatcgaaa tccagatcct
      181 tgacccgcag ttgcaaaccc tcactgatcc gcatgcccgt tccatacaga agctgggcga
      241 acaaacgatg ctcgccttcc agaaaaccga ggatgcgaac cacttcatcc ggggtcagca
      301 ccaccggcaa gcgccgcgac ggccgaggtc ttccgatctc ctgaagccag ggcagatccg
      361 tgcacagcac cttgccgtag aagaacagca aggccgccaa tgcctgacga tgcgtggaga
      421 ccgaaacctt gcgctcgttc gccagccagg acagaaatgc ctcgacttcg ctgctgccca
      481 aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggac
//
LOCUS       DAEEUE010000300          534 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.313, whole genome shotgun sequence.
ACCESSION   DAEEUE010000300 DAEEUE010000000
VERSION     DAEEUE010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.313"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..>534)
                     /locus_tag="KLK84_004334"
     CDS             complement(<1..>534)
                     /locus_tag="KLK84_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBE2011390.1"
                     /translation="NLSSAAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVF
                     SKDANQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRL
                     MGRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKTEQELFDA
                     MKKGALKSADTLQKVTQ"
BASE COUNT          149 a          132 c           93 g          160 t
ORIGIN      
        1 tcctgagtga ccttctgaag agtatcggca gattttaaag cccccttctt catagcatca
       61 aataattcct gttcagtctt tccggttgct ttagcgaata cctgaaccat accaggaaca
      121 gcttcagcta gttgcccctt aagctcttca gccataatct gacccttacc agccatctgt
      181 gataatgctg tcagtgctcg cttaatctct tcttcacttc gccccatcag acgagaatag
      241 ccaatcatat ttctaaaaag ttcttcagaa ccttgaatgc ctagagactc tctagctgaa
      301 gcaccgaaac cagcaaactg tgattgcagt tcagcgatat ctaaacccgt tgtttgagct
      361 aatgactttg tgaaatcttt tacttgattt gcatctttag aaaatacgaa atcaattgaa
      421 cgttcagcac ttgcacgttg aaggccaact tccattactc gctgataagc ggatagtaca
      481 gcctgaaagc tgataagtac acctgctgtt ttaactgctg ctgaacttaa atta
//
LOCUS       DAEEUE010000301          529 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.314, whole genome shotgun sequence.
ACCESSION   DAEEUE010000301 DAEEUE010000000
VERSION     DAEEUE010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.314"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            1..238
                     /locus_tag="KLK84_004335"
                     /pseudo
     CDS             1..238
                     /locus_tag="KLK84_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            338..>529
                     /locus_tag="KLK84_004336"
     CDS             338..>529
                     /locus_tag="KLK84_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011391.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTSTTLDYLLVKSG
                     KKLSNYFKAVATNFPVVNDL"
BASE COUNT          181 a          109 c          104 g          135 t
ORIGIN      
        1 aacaaatggt agtgtccgct attgccagta cacctcatat tggctacgta attaaaacag
       61 aaaataaaga aaccccattc actgtagttg atatcgacgg tctatcagga aactttaaaa
      121 ctcttgttga aggtgccaca aaaatgtgcc tagtttacat cggaaataat ctgcccgcag
      181 aaaaaaagcc gaatttctgg caaatctgat tgcaatgaaa ttaaaaggtg aaatctgaaa
      241 aaaagaaagc ctgcacagtg tgcaggcctg agtgaagaac ctgggacatt tattcatcac
      301 tcgcagtaat tttaatctga gttgaggtta aaaaacaatg agcacaaaac cactcttcct
      361 gttacggaaa gcgaaaaaat catccggtga acctgacgtc gtcctgtggg caagcgacga
      421 ttttgaatcg accagcacca ctctggacta tctgctcgtt aagtcaggta aaaaactgag
      481 caactatttt aaagctgttg ccacaaattt tcctgtcgtt aatgacctt
//
LOCUS       DAEEUE010000302          516 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.315, whole genome shotgun sequence.
ACCESSION   DAEEUE010000302 DAEEUE010000000
VERSION     DAEEUE010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.315"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            3..170
                     /gene="xisR"
                     /locus_tag="KLK84_004337"
     CDS             3..170
                     /gene="xisR"
                     /locus_tag="KLK84_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014884030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HBE2011392.1"
                     /translation="MLRTGLGARQIEAYRQNCWVEGFHFKRVSPLGKPDSKRGIIWYN
                     YPKINQFIKDS"
     gene            172..342
                     /locus_tag="KLK84_004338"
                     /pseudo
     CDS             172..342
                     /locus_tag="KLK84_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
     gene            complement(359..>516)
                     /locus_tag="KLK84_004339"
     CDS             complement(359..>516)
                     /locus_tag="KLK84_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE2011393.1"
                     /translation="ISKRYTQRIERHNLNLRQHLARLVRKSLSFSKSVELHDKVIGHY
                     LNIKHYQ"
BASE COUNT          150 a          103 c          123 g          140 t
ORIGIN      
        1 tgatgcttcg cactggtctg ggtgccagac aaattgaagc ataccgccag aactgttggg
       61 tggaaggctt ccacttcaaa cgagtatctc ctttagggaa gccagacagt aagcgaggga
      121 ttatctggta caactatccg aagataaatc agtttatcaa agactcatga tatgtctaaa
      181 ttaccaacag gtgtcgagat tcgaggtaaa tacattcgca tctggttcat gtttcgagga
      241 aaacgatgtc gggaaacatt gaaaggctgg gagattacta acagtaacat taaaaaggcc
      301 gggaatttaa gagcgttgat agttcatcaa atcagttccg gtgggtaatg actccaactt
      361 actgatagtg ttttatgttc agataatgcc cgatgacctt gtcatgcagc tccaccgatt
      421 ttgagaacga cagtgacttc cgtaccagcc ttgccagatg ttgtctcaga ttcagattat
      481 gtcgctcaat gcgctgagtg taacgcttgc tgataa
//
LOCUS       DAEEUE010000303          500 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.316, whole genome shotgun sequence.
ACCESSION   DAEEUE010000303 DAEEUE010000000
VERSION     DAEEUE010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.316"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(102..>500)
                     /locus_tag="KLK84_004340"
     CDS             complement(102..>500)
                     /locus_tag="KLK84_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE2011394.1"
                     /translation="NAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLR
                     LMKNGYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGH
                     SSGSITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          144 a          145 c           95 g          116 t
ORIGIN      
        1 gtgagtcgta ttaattatcc cttgacaagt agagaaaacc atgagtcaaa aacatgacca
       61 cacaggtctg tagtaagtgt catcctagac tatggtctcc tctaggtctc cttataaaca
      121 tcagccatca cattgaccag cctatcaacc tcgacctctt tagcataacg gtcgtacgta
      181 atagaccctg aactatgccc catgatcgcc tgtgcatacg ccaactcgac cccatgttgt
      241 tttaactggg tcgcaactgt atgtctaaac gagtgaaacg ctaagccgct acggtctacg
      301 aggactttag gcaacaacgt ccgattaaac catttactca cctgctcacc gtaaccgttc
      361 ttcatgagac gcagaccatc gaacaccata gcgtcatcac cgtcagcgcc tctacgggtc
      421 tctacaagcg ccataaagtc agccaatata aaaccatagg ccccatcgac cagaggcaca
      481 cagcggtcac tatgagcgtt
//
LOCUS       DAEEUE010000304          491 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.317, whole genome shotgun sequence.
ACCESSION   DAEEUE010000304 DAEEUE010000000
VERSION     DAEEUE010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.317"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            1..491
                     /locus_tag="KLK84_004341"
                     /pseudo
     CDS             1..491
                     /locus_tag="KLK84_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          124 a          139 c          136 g           92 t
ORIGIN      
        1 cagcccgtga actcagcctg tatgaatcac agctctacaa ctggcgcagt aaacagcaaa
       61 atcagcagac gtcttctgaa cgtgaactgg agatgtctac cgagattgca cgtctcaaac
      121 gccagctggc agaacgggat gaagagctgg ctatcctcca aaaggccgcg acatacttcg
      181 cgaagcgcct gaaatgaagt atgtctttat tgaaaaacat caggctgagt tcagcatcaa
      241 agcaatgtgc cgcgtgctcc gggtggcccg cagcggctgg tatacgtggt gtcagcggcg
      301 gacaaggata agcacgcgtc agcagttccg ccaacactgc gacagcgttg tcctcgcggc
      361 ttttacccgg tcaaaacagc gttacggtgc cccacgcctg acggatgaac tgcgtgctca
      421 gggttacccc tttaacgtaa aaaccgtggc ggcaagcctg cgccgtcagg gactgagggc
      481 aaaggcctcc c
//
LOCUS       DAEEUE010000305          447 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.318, whole genome shotgun sequence.
ACCESSION   DAEEUE010000305 DAEEUE010000000
VERSION     DAEEUE010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.318"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..>447
                     /locus_tag="KLK84_004342"
     CDS             <1..>447
                     /locus_tag="KLK84_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBE2011395.1"
                     /translation="ATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEV
                     GGNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKA
                     PGAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSN"
BASE COUNT          130 a           95 c          116 g          106 t
ORIGIN      
        1 acgccaccta ttctgtttcg caaaacgata attccggtag ctcacatcaa ctcggactca
       61 gcggtacggc gctggaaaga aataaccttt catggaattt aatgcagtcc tataacagtc
      121 atgatgatga ggttggcggt aatatgtccc tgacctatga tggctcttat ggcacggtga
      181 acggcagcta taactacagc caaaattccc agaggctgaa ttatggtatc agagggggaa
      241 ttctggcaca cagcgaaggg gtaacgttaa gtcaggagtt aggtgaaact attgctcttg
      301 ttaaagcacc tggggccgcc gggttagaaa tagataatat gcgcggtgct gcgacggact
      361 ggcgcggcta tacggtcaag acacagctaa acccttatga tgaaaatcgg gtagcaatca
      421 gcgataacta tttctcgaag tcgaata
//
LOCUS       DAEEUE010000306          446 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.319, whole genome shotgun sequence.
ACCESSION   DAEEUE010000306 DAEEUE010000000
VERSION     DAEEUE010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.319"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..>446)
                     /locus_tag="KLK84_004343"
     CDS             complement(<1..>446)
                     /locus_tag="KLK84_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HBE2011396.1"
                     /translation="ILSNRVASYGAIMAALPYMQLYIADYLADTMHLSAEEHGAYLLL
                     MFNYWQTGKPIPKNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVR
                     EKLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVGVVFEH"
BASE COUNT          102 a          113 c           86 g          145 t
ORIGIN      
        1 catgttcgaa caccactcca acatctgttt gaacacctgt taaatttctc tcttgttttg
       61 tttgaacatc tgcttccttt ctgcttcttc tggcctgaac agatgctttt cctgcggctg
      121 attttttggt taatttttct ctgacagatg ccagatcttc ctcaatccga agatgcatcc
      181 attcgtcgcc gttatcacaa aaaaactccc gcaaggatgg ttcaacatca acccatcgct
      241 cgttagtcag acgggcaatt tttgccaacc tgtttttggg tattggcttt cctgtttgcc
      301 agtaattgaa catcagcaac aaatacgcgc cgtgctcctc tgctgacaaa tgcatggtgt
      361 cagccaggta atcagctatg tacagttgca tgtatggtaa tgcggccata attgccccgt
      421 atgatgctac ccggttgctt agaata
//
LOCUS       DAEEUE010000307          446 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.320, whole genome shotgun sequence.
ACCESSION   DAEEUE010000307 DAEEUE010000000
VERSION     DAEEUE010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.320"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..395)
                     /locus_tag="KLK84_004344"
     CDS             complement(<1..395)
                     /locus_tag="KLK84_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011397.1"
                     /translation="MPVFHNTRVLVEPEPKSMRNLPSGVVPAVRQPLVEDKTLLPFFS
                     NARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAHQNLSAWMIDVIGHAISGT"
BASE COUNT          105 a          123 c          123 g           95 t
ORIGIN      
        1 gtaccgctta ttgcgtgacc gatgacgtca atcatccatg ctgacaggtt ttgatgagca
       61 agttgctcga gtgattcgga tgtctggtca cgcagctggt tgtcgcagtg ccagcacaat
      121 accattgcgc cggtaccata acggtgaatg acggtttcgc tgtgatgata atcgccgtgt
      181 ggccactggc aggatttaat atggcgcaac agccagtcag acaatgcgcc agcaccacca
      241 gcagcacgaa tcacccgtgc gttactgaaa aacggcagca atgttttgtc ttccactagc
      301 ggctggcgaa cggcaggaac gaccccggac ggcagattac gcatgctttt cggttccggc
      361 tccaccagta cccgggtatt gtggaatacc ggcatggatt cacgaccggg cttaaggacc
      421 accagcccga gttccggaac cagaac
//
LOCUS       DAEEUE010000308          445 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.321, whole genome shotgun sequence.
ACCESSION   DAEEUE010000308 DAEEUE010000000
VERSION     DAEEUE010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.321"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..374)
                     /locus_tag="KLK84_004345"
     CDS             complement(<1..374)
                     /locus_tag="KLK84_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE2011398.1"
                     /translation="MVSNTQLLEQRIADFFTLSDEHKKARVLLDTLACSCPARIFGGM
                     VRDLGLYGVDGFSSDLDIVIGRSREELFQTLAELPVKQLRFNKFGGIRFRYHDFEFDI
                     WNLNETWAFQEKLIFCEDESSL"
BASE COUNT          135 a          106 c           95 g          109 t
ORIGIN      
        1 aacagacttg attcatcctc acaaaatata agtttttcct ggaatgccca tgtctcattg
       61 aggttccaga tatcaaattc gaagtcgtga taacgaaaac ggatcccacc aaacttatta
      121 aaacgcagtt gtttgacggg cagttcagcc aatgtctgga agagttcctc acgggagcga
      181 ccgatgacga tatcaaggtc tgaagagaat ccatcgacgc catacaagcc taagtcacgc
      241 accatcccgc caaaaatcct ggccggacag gaacaggcca gagtatcaag taatactctg
      301 gctttcttat gctcatcact aagcgtaaaa aaatcggcga tacgctgttc aagcagttgg
      361 gtgtttgaga ccacattcat acagtgattt tagctcatcg tgaaggcgca ttaaataact
      421 atccgtcatg cctgagattg cgtcg
//
LOCUS       DAEEUE010000309          440 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.denovo.322, whole genome shotgun sequence.
ACCESSION   DAEEUE010000309 DAEEUE010000000
VERSION     DAEEUE010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.denovo.322"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            69..>440
                     /locus_tag="KLK84_004346"
     CDS             69..>440
                     /locus_tag="KLK84_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBE2011399.1"
                     /translation="MSLFRRGEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGIQLRDITESK
                     IYSAMQKMTNRRHEENWKLRAE"
BASE COUNT          152 a           88 c          114 g           86 t
ORIGIN      
        1 gcggctcttc agtctccgct gcatactgtc caggtgagcg cgggtgatgg cataacagag
       61 gaaagaaaat gtcactcttc cgcagaggtg aaatatggta cgccagtttc acattgccga
      121 acggtaaaag atttaaacag tctcttggaa caaaggacaa aaggcaggcg acagaactcc
      181 atgacaagct aaaggctgaa gcatggcggg tcagcaaact tggtgaaata cctgatataa
      241 cgttcgagga agcgtgtgtc aggtggcttg aagagaaagc acataaaaaa tcactggacg
      301 atgacaaaag ccggatcgga ttctggcttc aacatttcgc aggaatacaa ctaagagaca
      361 ttactgaatc aaaaatttat tcagcaatgc agaaaatgac gaaccggcgt catgaggaaa
      421 actggaaact cagggcagaa
//
LOCUS       DAEEUE010000310         2901 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.guided.220, whole genome shotgun sequence.
ACCESSION   DAEEUE010000310 DAEEUE010000000
VERSION     DAEEUE010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.guided.220"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            1..349
                     /locus_tag="KLK84_004347"
                     /pseudo
     CDS             1..349
                     /locus_tag="KLK84_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(406..1863)
                     /gene="pepD"
                     /locus_tag="KLK84_004348"
     CDS             complement(406..1863)
                     /gene="pepD"
                     /locus_tag="KLK84_004348"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HBE2011400.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2125..2583
                     /gene="gpt"
                     /locus_tag="KLK84_004349"
     CDS             2125..2583
                     /gene="gpt"
                     /locus_tag="KLK84_004349"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBE2011401.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2675..>2901
                     /locus_tag="KLK84_004350"
     CDS             2675..>2901
                     /locus_tag="KLK84_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBE2011402.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVM"
BASE COUNT          698 a          743 c          744 g          716 t
ORIGIN      
        1 aaactggttt ctgggcattg ggcgttttac cgctgatgag caggagcaat ccgatgcgat
       61 ccggtatgag acgctgcgtt cgtcggggcc gggcggtcaa catgtcaata aaaccgactc
      121 ggcggtacgc gccacgcatt tggcatccgg tattagcgtg aaggttcagt cagagcgcag
      181 ccagcatgct aacaaacggc tggcgcgatt gctgattgcc tggaagctgg atcagcagca
      241 ataggaaaat agcgcggtgc tgaaatcaca gcggcgaatg ttccatcacc agattgaacg
      301 tggcaacccg cgacgaacgt ttacagggat ggcttttatc gaaggataat gaaggatgaa
      361 actgccggaa ggcgattaaa cgccatccgg cagcaaatca aataattact tcgccggaat
      421 ttctttcagc agttcagtca gcagtgtcca gtaatgacct acgctttcga tgtgaacttg
      481 ctcatccgga gagtgtggac cggtgatagt tggcccgata gaaaccatgt ccatttccgg
      541 atacggtttt ttgaacagac cacattccag gcccgcgtgg ataatctgga tgttcggcgt
      601 cttgttgaac aggcgctgat aggtttcacg taccagatgc atcaccggag aattagcgtc
      661 cggctgccag ccaggatatg cgcctttcgc ttcggttttc gcgccagcca gtttacccag
      721 cgaatccagc atgctcacca cgtagtcttt accgctgtcg atcagtgaac ggatcaggca
      781 gtgaatttct acgttattgt cagtcatggt caccacaccg acgttcaggg aggtttcgac
      841 cacacctttt gccacgtcgg aattgcggat cacaccgttc ggggtggcgt tcagcagacg
      901 aataaaggta tcgcgagatt tcgcaatcag ggcagcttta tcgttcgcta cagagtccag
      961 caacaaggcc agatttttct ctttctctgc cagctcgttt ttcaggatct cctgataggt
     1021 attcaccaga gatttcagtg cgtcgacttt atcagctgcg acagcaatgg tcgcaaaggc
     1081 ttcacgcggg atggcgttac gcagtgtgcc gccgttgaaa tcgataaggc gcaggtccag
     1141 ttcttctgca tgacccgcca ggaagcgcac cagcagtttg ttggcattac ccaggccaac
     1201 gtggatttca ccgccggagt gaccgccttt cagacctttt aaggttaact tgaaggtttc
     1261 aaaaccagct ggaaccgctt cacgatctaa atgcaggttg gaggtgaagt cgataccccc
     1321 cgcacaaccc atgtagattt caccttcttc ttcggagtcg gtgttaatca gaatatcagc
     1381 ctgcaaccag ttgctctgta agccgaacgc accgtccata ccggcttctt cggtcatggt
     1441 cagcagcact tccagcgggc cgtgaaccac gttttcgtca gccagaaccg ccagtgcaga
     1501 ggccatacca atgccgttat ccgcacccag cgtggtgccg cgcgctttaa cccattcgcc
     1561 atcaatataa ggctggatag gatctttcgt gaagtcatgc acggtgtcgt tatttttctg
     1621 cggcaccata tcgaggtggg cctgtaagac gaccggttta cgattttcca tacctgcggt
     1681 agcaggttta cgaatcagga tattacctac ctgatcgcgt tcgacatgga aacctttctc
     1741 ttttgcccaa ccaacaatgt attcagcgag ttgctcttca tgataggacg ggtgaggaat
     1801 agaacagatt ttggcaaaaa tatcccacag cggctgtgga gataattgag acagttcaga
     1861 cacgttaagt ctccttgtcg atcacccgca aaacagtatt gcaggtcaca gggttagcag
     1921 aaaatgttgt caacacaaga caggcttgcg agatatgtat tgagaatacc actttatccc
     1981 gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt
     2041 gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta
     2101 gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg
     2161 cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt
     2221 attgccgtaa gccgtggcgg tctggtaccg ggggcgttac tggcgcgtga actgggtatt
     2281 cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa
     2341 gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat
     2401 accggtggta ctgctgttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc
     2461 ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat
     2521 acctggatcg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc
     2581 taatcttttc aacgcctggc attgccgggc gttgttcttt ttaacttcag gcaggttaca
     2641 atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac
     2701 cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca
     2761 cggcgcacaa ccgcctgtgc agtcggctct tgatggtaaa accatccctc actggtatcg
     2821 catgattaac cgtctgatgt ggatctggcg cggtattgac ccacgtgaaa tcctcgacgt
     2881 ccaggcacgt attgtgatga g
//
LOCUS       DAEEUE010000311         2655 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.guided.227, whole genome shotgun sequence.
ACCESSION   DAEEUE010000311 DAEEUE010000000
VERSION     DAEEUE010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.guided.227"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..375
                     /gene="frdC"
                     /locus_tag="KLK84_004351"
     CDS             <1..375
                     /gene="frdC"
                     /locus_tag="KLK84_004351"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBE2011403.1"
                     /translation="YVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELIFGLFALK
                     NGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDEKMGPEPI
                     IKSLWAVTVVATIVILFVALYW"
     gene            386..745
                     /gene="frdD"
                     /locus_tag="KLK84_004352"
     CDS             386..745
                     /gene="frdD"
                     /locus_tag="KLK84_004352"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBE2011404.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            808..1941
                     /locus_tag="KLK84_004353"
     CDS             808..1941
                     /locus_tag="KLK84_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBE2011405.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            2030..2563
                     /gene="blc"
                     /locus_tag="KLK84_004354"
     CDS             2030..2563
                     /gene="blc"
                     /locus_tag="KLK84_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HBE2011406.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
BASE COUNT          637 a          676 c          688 g          654 t
ORIGIN      
        1 tatgtacggc caatgacgtc cacctggtgg aaaaaattgc cgttttatcg cttttacatg
       61 ctgcgcgaag gcacggcggt tccggctgtg tggttcagca ttgaactgat tttcgggctg
      121 tttgccctga aaaatggccc ggaagcctgg gcgggattcg tcgacttttt acaaaacccg
      181 gttatcgtga tcattaacct gatcactctg acggcagctc tgctgcacac caaaacctgg
      241 tttgaactgg caccgaaagc ggccaatatc attgtaaaag acgaaaaaat gggaccagag
      301 ccaattatca aaagtctctg ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt
      361 gccctgtact ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc
      421 ggtattctgg ggcctcttcg gggccggtgg tatgtggagc gcaattattg cgccggtgat
      481 gatcctgctg gtgggtattc tgctgccact ggggctgttt ccgggtgatg cgctgagcta
      541 cgagcgcgtt ctggcgttcg cgcagagctt cattggtcgc gtattcctgt tcctgatgat
      601 cgttctgccg ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg atctgaaaat
      661 ccacgtgcct gcgggcaaat gggttttcta cggtctggct gctatcctga cagttgtcac
      721 gctgattggt gtcgttacaa tctaacgcat cgccaatgta aatccggccc gcctatggcg
      781 ggccgttttg tatggaaacc agaccttatg ttcaaaacga cgctctgcgc cttattaatt
      841 accgcctctt actccacatt tgctgcccct caacaaatca acgatattgt gcatcgcaca
      901 attaccccgc ttatagagca acaaaagatc cccggtatgg cggtggcggt aatttatcag
      961 ggtaaacctt attactttac ctggggctat gcggacatcg ccaaaaagca gcccgtcaca
     1021 cagcaaacgt tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt gcttggtggc
     1081 gacgctattg ctcgagggga aatcaagtta agcgatcccg caacaaaata ctggcctgaa
     1141 cttaccgcta aacagtggaa tgggatcaca ctattacatc ttgcgaccta caccgctggc
     1201 ggcctgccat tgcaggtgcc ggatgaagtg aaatcctcaa gcgacttgct gcgcttctat
     1261 caaaactggc agcctgcatg ggctccagga acacaacgtc tgtatgccaa ctccagtatc
     1321 ggtttgttcg gcgcactggc tgtgaagccg tctggtttga gttttgagca ggcgatgcaa
     1381 actcgtgtct tccagccact caaactcaac catacgtgga ttaatgtacc tcccccagaa
     1441 gaaaagaatt acgcctgggg atatcgcgaa ggtaaggcag tgcatgtttc gccaggggcg
     1501 ttagatgctg aagcttatgg tgtgaagtcg accattgaag atatggcccg ctgggtgcaa
     1561 agcaatttac ccccccttga tatcaatgag aaaacgcttc aacaagggat acaactggca
     1621 caatctcgct actggcaaac cggcgatatg tatcagggcc tgggctggga aatgctggac
     1681 tggccggtca atcctgacag catcattaac ggcagtgaca ataaaattgc actggcagca
     1741 cgcccggtaa aagcgattaa gcccccaact cctgcagtac gcgcatcatg ggtacataaa
     1801 acgggggcga ccggcggatt tggtagctat gtcgcgttta ttccagaaaa agagctgggt
     1861 atcgtgatgc tggctaacaa aaactatccc aatccagcga gagtcgccgc cgcctgtcag
     1921 attctcaacg ctctacagta aaatttcatc gggtccgaat tttcggacct tttctgcgct
     1981 tttccttgct gtcatctaca cttagaaaaa aaccagtaag gaaacattta tgcgcctgct
     2041 ccctctcgtt gccgcagcga cagctgcatt tctggtcgtt gcctgtagtt ttcctacgcc
     2101 gccgcgtggc gtgaccgtag tgaataattt cgacgccaaa cgttatctcg gtacctggta
     2161 tgagattgcc cgttttgatc accgttttga acgtggactg gaaaaagtca ccgcaacata
     2221 cagcctgcgt gatgacggcg gcctgaatgt cattaataaa ggctataacc ctgacagagg
     2281 aatgtggcag cagagtgaag ggaaagcgta ctttaccggc gcaccaactc gcgctgcgct
     2341 gaaagtgtca ttctttggtc ctttctatgg cggttataac gttattgcac tcgatcggga
     2401 ataccgccat gcgctggttt gcggcccgga ccgcgactac ctgtggatac tctcccgcac
     2461 gccaaccatt tctgacgaag tgaaacagga gatgctggca gtcgcgaccc gggaagggtt
     2521 tgatgtcagt aagtttattt gggtacagca gcccggtagt tagtgagtgc tgagtttcag
     2581 accaataatc cccaatacga ttaacgccag actcgccaga cgcatcgggt tagcggactc
     2641 accgagcagc acaat
//
LOCUS       DAEEUE010000312         2626 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.guided.229, whole genome shotgun sequence.
ACCESSION   DAEEUE010000312 DAEEUE010000000
VERSION     DAEEUE010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.guided.229"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            109..1014
                     /locus_tag="KLK84_004355"
     CDS             109..1014
                     /locus_tag="KLK84_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HBE2011407.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            1011..2249
                     /locus_tag="KLK84_004356"
     CDS             1011..2249
                     /locus_tag="KLK84_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE2011408.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            2249..>2626
                     /locus_tag="KLK84_004357"
     CDS             2249..>2626
                     /locus_tag="KLK84_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBE2011409.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAV"
BASE COUNT          407 a          835 c          848 g          536 t
ORIGIN      
        1 gacaaaaggc tgcctcatcg ctaactttgc aacagtgccg gattgaatat aaccgacgtg
       61 actgttacat ttaggtggct aaacccgtca agccctcagg agtgaatcat gaccgtagtc
      121 acgaccgccg atacctccca actgtacgca cttgcagccc gacatgggct caagctccat
      181 ggcccgctga ctgtcaatga gcttgggctc gactatagga tcgtgatcgc caccgtcgac
      241 gatggacgtc ggtgggtgct gcgcatcccg cgccgagccg aggtaagcgc gaaggtcgaa
      301 ccagaggcgc gggtgctggc aatgctcaag aatcgcctgc cgttcgcggt gccggactgg
      361 cgcgtggcca acgccgagct cgttgcctat cccatgctcg aagactcgac tgcgatggtc
      421 atccagcctg gttcgtccac gcccgactgg gtcgtgccgc aggactcgga ggtcttcgcg
      481 gagagcttcg cgaccgcgct cgccgccctg catgccgtcc ccatttccgc cgccgtggat
      541 gcggggatgc tcatccgtac accgacgcag gcccgtcaga aggtggccga cgacgttgac
      601 cgcgtccgac gcgagttcgt ggtgaacgac aagcgcctcc accggtggca gcgctggctc
      661 gacgacgatt cgtcgtggcc agatttctcc gtggtggtgc atggcgatct ctacgtgggc
      721 catgtgctca tcgacaacac ggagcgcgtc agcgggatga tcgactggag cgaggcccgc
      781 gttgatgacc ctgccatcga catggccgcg caccttatgg tctttggtga agaggggctc
      841 gcgaagctcc tcctcacgta tgaagcggcc ggtggccggg tgtggccgcg gctcgcccac
      901 cacatcgcgg agcgccttgc gttcggggcg gtcacctacg cactcttcgc cctcgactcg
      961 ggtaacgaag agtacctcgc tgcggcgaag gcgcagctcg ccgcagcgga atgagcgaac
     1021 gtcgatatag cccgctcgcg acgctgttcg cggcgacctt tctcttccgg atcggcaacg
     1081 cggtggcggc cctcgcgctt ccatggttcg tcctgtctca tacaaagagc gcggcctggg
     1141 cgggcgccac ggccgctagc agcgtcatcg cgaccatcat cggcgcgtgg gttggtggtg
     1201 gcctcgtcga tcggttcggg cgcgcgcccg tcgcattgat ctcgggtgtg gtgggcggcg
     1261 tggccatggc gagcatccca ctgctcgatg ccgttggcgc cctctcgaac actgggctga
     1321 tcgcttgcgt ggtgctcggt gccgcgttcg acgcacccgg tatggccgcg caggacagtg
     1381 agctgcccaa actcggccac gtcgccgggc tctccgttga gcgcgtctcg tcactgaaag
     1441 cggtgatcgg gaacgtcgcg attctaggtg gcccggccct tgggggggcc gcaatcggcc
     1501 tgcttggcgc tgcgccaacg ctcgggctga cggcgttctg ctccgtcctt gcaggtctgc
     1561 tcggcgcgtg ggtgcttccc gcgcgtgccg ctcggacgat gaccacgacg gcgactctct
     1621 ccatgcgcgc cggcgtcgct tttctctgga gcgaacccct gctgcgccct ctctttggta
     1681 tagtgatgat cttcgtgggc atcgttggcg ccaacggcag cgtcatcatg cctgcgctgt
     1741 ttgtagatgc aggacgccaa gtagcagagc tcgggctgtt ctcctcaatg atgggggctg
     1801 gtggtctcct tggcattgcc attcatgcgt cggtcggcgc ccggatatca gcgcagaact
     1861 ggctggcggt ggcattttgt ggctctgcgg tgggctcgct tctgctttca cagttgccag
     1921 gcgtgccggt gctgatgttg ttgggcgcgc tcgtgggact gctgaccggc tcagtctctc
     1981 ccattctcaa cgctgccatc tacaaccgca cgccgccaga acttctcggc cgggtactcg
     2041 gcacggtctc ggcggtgatg ctgtcagcct cgcccatggt tatgcttgcg gccggcgcgt
     2101 ttgtcgacct tgctggtccg ctccctggcc tcgttgtatc ggccgtgttt gcggggctcg
     2161 tggctctact ctcgctccgt cttcaatttg ctacaatggc ggcggcagcc acagcctccg
     2221 ccccaaccca tacagaaggt gaacactgat gccccgcccc aagctcaagt ccgatgacga
     2281 ggtactcgag gccgccaccg tagtgctgaa gcgttgcggt cccatagagt tcacgctcag
     2341 cggagtagca aaggaggtgg ggctctcccg cgcagcgtta atccagcgct tcaccaaccg
     2401 cgatacgctg ctggtgagga tgatggagcg cggcgtcgag caggtgcggc attacctgaa
     2461 tgcgataccg ataggcgcag ggccgcaagg gctctgggaa tttttgcagg tgctcgttcg
     2521 gagcatgaac actcgcaacg acttctcggt gaactatctc atctcctggt acgagctcca
     2581 ggtgccggag ctacgcacgc ttgcgatcca gcggaaccgc gcggtg
//
LOCUS       DAEEUE010000313         2626 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.guided.230, whole genome shotgun sequence.
ACCESSION   DAEEUE010000313 DAEEUE010000000
VERSION     DAEEUE010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.guided.230"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            109..1014
                     /locus_tag="KLK84_004358"
     CDS             109..1014
                     /locus_tag="KLK84_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HBE2011410.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVMATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            1011..2249
                     /locus_tag="KLK84_004359"
     CDS             1011..2249
                     /locus_tag="KLK84_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE2011411.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            2249..>2626
                     /locus_tag="KLK84_004360"
     CDS             2249..>2626
                     /locus_tag="KLK84_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBE2011412.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAV"
BASE COUNT          407 a          834 c          849 g          536 t
ORIGIN      
        1 gacaaaaggc tgcctcatcg ctaactttgc aacagtgccg gattgaatat aaccgacgtg
       61 actgttacat ttaggtggct aaacccgtca agccctcagg agtgaatcat gaccgtagtc
      121 acgaccgccg atacctccca actgtacgca cttgcagccc gacatgggct caagctccat
      181 ggcccgctga ctgtcaatga gcttgggctc gactatagga tcgtgatggc caccgtcgac
      241 gatggacgtc ggtgggtgct gcgcatcccg cgccgagccg aggtaagcgc gaaggtcgaa
      301 ccagaggcgc gggtgctggc aatgctcaag aatcgcctgc cgttcgcggt gccggactgg
      361 cgcgtggcca acgccgagct cgttgcctat cccatgctcg aagactcgac tgcgatggtc
      421 atccagcctg gttcgtccac gcccgactgg gtcgtgccgc aggactcgga ggtcttcgcg
      481 gagagcttcg cgaccgcgct cgccgccctg catgccgtcc ccatttccgc cgccgtggat
      541 gcggggatgc tcatccgtac accgacgcag gcccgtcaga aggtggccga cgacgttgac
      601 cgcgtccgac gcgagttcgt ggtgaacgac aagcgcctcc accggtggca gcgctggctc
      661 gacgacgatt cgtcgtggcc agatttctcc gtggtggtgc atggcgatct ctacgtgggc
      721 catgtgctca tcgacaacac ggagcgcgtc agcgggatga tcgactggag cgaggcccgc
      781 gttgatgacc ctgccatcga catggccgcg caccttatgg tctttggtga agaggggctc
      841 gcgaagctcc tcctcacgta tgaagcggcc ggtggccggg tgtggccgcg gctcgcccac
      901 cacatcgcgg agcgccttgc gttcggggcg gtcacctacg cactcttcgc cctcgactcg
      961 ggtaacgaag agtacctcgc tgcggcgaag gcgcagctcg ccgcagcgga atgagcgaac
     1021 gtcgatatag cccgctcgcg acgctgttcg cggcgacctt tctcttccgg atcggcaacg
     1081 cggtggcggc cctcgcgctt ccatggttcg tcctgtctca tacaaagagc gcggcctggg
     1141 cgggcgccac ggccgctagc agcgtcatcg cgaccatcat cggcgcgtgg gttggtggtg
     1201 gcctcgtcga tcggttcggg cgcgcgcccg tcgcattgat ctcgggtgtg gtgggcggcg
     1261 tggccatggc gagcatccca ctgctcgatg ccgttggcgc cctctcgaac actgggctga
     1321 tcgcttgcgt ggtgctcggt gccgcgttcg acgcacccgg tatggccgcg caggacagtg
     1381 agctgcccaa actcggccac gtcgccgggc tctccgttga gcgcgtctcg tcactgaaag
     1441 cggtgatcgg gaacgtcgcg attctaggtg gcccggccct tgggggggcc gcaatcggcc
     1501 tgcttggcgc tgcgccaacg ctcgggctga cggcgttctg ctccgtcctt gcaggtctgc
     1561 tcggcgcgtg ggtgcttccc gcgcgtgccg ctcggacgat gaccacgacg gcgactctct
     1621 ccatgcgcgc cggcgtcgct tttctctgga gcgaacccct gctgcgccct ctctttggta
     1681 tagtgatgat cttcgtgggc atcgttggcg ccaacggcag cgtcatcatg cctgcgctgt
     1741 ttgtagatgc aggacgccaa gtagcagagc tcgggctgtt ctcctcaatg atgggggctg
     1801 gtggtctcct tggcattgcc attcatgcgt cggtcggcgc ccggatatca gcgcagaact
     1861 ggctggcggt ggcattttgt ggctctgcgg tgggctcgct tctgctttca cagttgccag
     1921 gcgtgccggt gctgatgttg ttgggcgcgc tcgtgggact gctgaccggc tcagtctctc
     1981 ccattctcaa cgctgccatc tacaaccgca cgccgccaga acttctcggc cgggtactcg
     2041 gcacggtctc ggcggtgatg ctgtcagcct cgcccatggt tatgcttgcg gccggcgcgt
     2101 ttgtcgacct tgctggtccg ctccctggcc tcgttgtatc ggccgtgttt gcggggctcg
     2161 tggctctact ctcgctccgt cttcaatttg ctacaatggc ggcggcagcc acagcctccg
     2221 ccccaaccca tacagaaggt gaacactgat gccccgcccc aagctcaagt ccgatgacga
     2281 ggtactcgag gccgccaccg tagtgctgaa gcgttgcggt cccatagagt tcacgctcag
     2341 cggagtagca aaggaggtgg ggctctcccg cgcagcgtta atccagcgct tcaccaaccg
     2401 cgatacgctg ctggtgagga tgatggagcg cggcgtcgag caggtgcggc attacctgaa
     2461 tgcgataccg ataggcgcag ggccgcaagg gctctgggaa tttttgcagg tgctcgttcg
     2521 gagcatgaac actcgcaacg acttctcggt gaactatctc atctcctggt acgagctcca
     2581 ggtgccggag ctacgcacgc ttgcgatcca gcggaaccgc gcggtg
//
LOCUS       DAEEUE010000314         2166 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.guided.238, whole genome shotgun sequence.
ACCESSION   DAEEUE010000314 DAEEUE010000000
VERSION     DAEEUE010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.guided.238"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            complement(<1..111)
                     /locus_tag="KLK84_004361"
     CDS             complement(<1..111)
                     /locus_tag="KLK84_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBE2011413.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVE"
     gene            193..1398
                     /gene="tet(B)"
                     /locus_tag="KLK84_004362"
     CDS             193..1398
                     /gene="tet(B)"
                     /locus_tag="KLK84_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HBE2011414.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1511..2104)
                     /gene="tetC"
                     /locus_tag="KLK84_004363"
     CDS             complement(1511..2104)
                     /gene="tetC"
                     /locus_tag="KLK84_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HBE2011415.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
BASE COUNT          560 a          394 c          451 g          761 t
ORIGIN      
        1 ctctacacct agcttctggg cgagtttacg ggttgttaaa ccttcgattc cgacctcatt
       61 aagcagctct aatgcgctgt taatcacttt acttttatct aatctagaca tcattaattc
      121 ctaatttttg ttgacactct atcattgata gagttatttt accactccct atcagtgata
      181 gagaaaagtg aaatgaatag ttcgacaaag atcgcattgg taattacgtt actcgatgcc
      241 atggggattg gccttatcat gccagtcttg ccaacgttat tacgtgaatt tattgcttcg
      301 gaagatatcg ctaaccactt tggcgtattg cttgcacttt atgcgttaat gcaggttatc
      361 tttgctcctt ggcttggaaa aatgtctgac cgatttggtc ggcgcccagt gctgttgttg
      421 tcattaatag gcgcatcgct ggattactta ttgctggctt tttcaagtgc gctttggatg
      481 ctgtatttag gccgtttgct ttcagggatc acaggagcta ctggggctgt cgcggcatcg
      541 gtcattgccg ataccacctc agcttctcaa cgcgtgaagt ggttcggttg gttaggggca
      601 agttttgggc ttggtttaat agcggggcct attattggtg gttttgcagg agagatttca
      661 ccgcatagtc ccttttttat cgctgcgttg ctaaatattg tcgctttcct tgtggttatg
      721 ttttggttcc gtgaaaccaa aaatacacgt gataatacag ataccgaagt aggggttgag
      781 acgcaatcga attcggtata catcacttta tttaaaacga tgcccatttt gttgattatt
      841 tatttttcag cgcaattgat aggccaaatt cccgcaacgg tgtgggtgct atttaccgaa
      901 aatcgttttg gatggaatag catgatggtt ggcttttcat tagcgggtct tggtctttta
      961 cactcagtat tccaagcctt tgtggcagga agaatagcca ctaaatgggg cgaaaaaacg
     1021 gcagtactgc tcggatttat tgcagatagt agtgcatttg cctttttagc gtttatatct
     1081 gaaggttggt tagttttccc tgttttaatt ttattggctg gtggtgggat cgctttacct
     1141 gcattacagg gagtgatgtc tatccaaaca aagagtcatc agcaaggtgc tttacaggga
     1201 ttattggtga gccttaccaa tgcaaccggt gttattggcc cattactgtt tgctgttatt
     1261 tataatcatt cactaccaat ttgggatggc tggatttgga ttattggttt agcgttttac
     1321 tgtattatta tcctgctatc gatgaccttc atgttaaccc ctcaagctca ggggagtaaa
     1381 caggagacaa gtgcttagtt atttcgtcac caaatgatgt tattccgcga aatataatga
     1441 ccctcttgat aacccaagag ggcatttttt acgataaaga agatttagct tcaaataaaa
     1501 cctatctatt ttatttatct ttcaagctca ataaaaagcc gcggtaaata gcaataaatt
     1561 ggcctttttt atcggcaagc tcttttaggt ttttcgcatg tattgcgata tgcataaacc
     1621 agccattgag taagttttta agcacatcat catcataagc tttaagttgg ttctcttgga
     1681 tcaatttgct gacaatggcg tttaccttac cagtaatgta ttcaaggcta attttttcaa
     1741 gttcattcca accaatgata ggcatcactt cttggatagg gataaggttt ttattattat
     1801 caataatata atcaagataa tgttcaaata tactttctaa ggcagaccaa ccatttgtta
     1861 aatcagtttt tgttgtgatg taggcatcaa tcataattaa ttgctgctta taacaggcac
     1921 tgagtaattg ttttttattt ttaaagtgat gataaaaggc acctttggtc accaacgctt
     1981 ttcccgagat ctcatctatt gaaacagctt gatagccttt ttcaacaaac aatattcgtg
     2041 ctgagttaac cagtgattga taggtactct taaaattttc ttgttgatga tttttatttt
     2101 ccatgataga tttaaaataa cataccgtca gtatgtttat ggtatcatga tgatgtggtc
     2161 gtgaca
//
LOCUS       DAEEUE010000315         1566 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.guided.256, whole genome shotgun sequence.
ACCESSION   DAEEUE010000315 DAEEUE010000000
VERSION     DAEEUE010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.guided.256"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..375
                     /locus_tag="KLK84_004364"
     CDS             <1..375
                     /locus_tag="KLK84_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HBE2011416.1"
                     /translation="PDFLPDEDKSTPQLDLLARVERELPVRLDQERTDMVVCHGDPCM
                     PNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEENWAAPDEAERAFAVLFNVL
                     GIEAPDRERLAFYLRLDPLTWG"
     gene            375..1211
                     /locus_tag="KLK84_004365"
     CDS             375..1211
                     /locus_tag="KLK84_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HBE2011417.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(1318..1566)
                     /locus_tag="KLK84_004366"
                     /pseudo
     CDS             complement(1318..1566)
                     /locus_tag="KLK84_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
BASE COUNT          344 a          452 c          431 g          339 t
ORIGIN      
        1 cccgacttct taccggacga ggacaagagt acgccgcagc tcgatctttt ggctcgtgtc
       61 gaacgagagc taccggtgcg gctcgaccaa gagcgcaccg atatggttgt ttgccatggt
      121 gatccctgca tgccgaactt catggtggac cctaaaactc ttcaatgcac gggtctgatc
      181 gaccttgggc ggctcggaac agcagatcgc tatgccgatt tggcactcat gattgctaac
      241 gccgaagaga actgggcagc gccagatgaa gcagagcgcg ccttcgctgt cctattcaat
      301 gtattgggga tcgaagcccc cgaccgcgaa cgccttgcct tctatctgcg attggaccct
      361 ctgacttggg gttgatgttc atgccgcctg tttttcctgc tcattggcac gtttcgcaac
      421 ctgttctcat tgcggacacc ttttccagcc tcgtttggaa agtttcattg ccagacggga
      481 ctcctgcaat cgtcaaggga ttgaaaccta tagaagacat tgctgatgaa ctgcgcgggg
      541 ccgactatct ggtatggcgc aatgggaggg gagcagtccg gttgctcggt cgtgagaaca
      601 atctgatgtt gctcgaatat gccggggagc gaatgctctc tcacatcgtt gccgagcacg
      661 gcgactacca ggcgaccgaa attgcagcgg aactaatggc gaagctgtat gccgcatctg
      721 aggaacccct gccttctgcc cttctcccga tccgggatcg ctttgcagct ttgtttcagc
      781 gggcgcgcga tgatcaaaac gcaggttgtc aaactgacta cgtccacgcg gcgattatag
      841 ccgatcaaat gatgagcaat gcctcggaac tgcgtgggct acatggcgat ctgcatcatg
      901 aaaacatcat gttctccagt cgcggctggc tggtgataga tcccgtcggt ctggtcggtg
      961 aagtgggctt tggcgccgcc aatatgttct acgatccggc tgacagagac gacctttgtc
     1021 tcgatcctag acgcattgca cagatggcgg acgcattctc tcgtgcgctg gacgtcgatc
     1081 cgcgtcgcct gctcgaccag gcgtacgctt atgggtgcct ttccgcagct tggaacgcgg
     1141 atggagaaga ggagcaacgc gatctagcta tcgcggccgc gatcaagcag gtgcgacaga
     1201 cgtcatacta gatatcaagc gacttctcct atcccctggg aacacatcaa tctcaccgga
     1261 gaatatcgct ggccaaagcc ttagcgtagg attccgcccc ttcccgcaaa cgaccccaaa
     1321 caggaaacgc agctgaaacg ggaagctcaa cacccactga cgcatgggtt gttcaggcag
     1381 tacttcatca accagcaagg cggcactttc ggccatccgc cgcgccccac agctcgggca
     1441 gaaaccgcga cgcttacagc tgaaagcgac caggtgctcg gcgtggcaag actcgcagcg
     1501 aacccgtaga aagccatgct ccagccgccc gcattggaga aattcttcaa attcccgttg
     1561 cacata
//
LOCUS       DAEEUE010000316         1345 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.guided.266, whole genome shotgun sequence.
ACCESSION   DAEEUE010000316 DAEEUE010000000
VERSION     DAEEUE010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.guided.266"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            <1..436
                     /locus_tag="KLK84_004367"
     CDS             <1..436
                     /locus_tag="KLK84_004367"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBE2011418.1"
                     /translation="MHSVQDGQADRREAPAGDIMDHIAAFFDARIAALTGAGIKRNRL
                     VLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRALTGRGPGDVGA
                     ATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            497..1300
                     /locus_tag="KLK84_004368"
                     /note="aph(3'')-Ib"
     CDS             497..1300
                     /locus_tag="KLK84_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HBE2011419.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
BASE COUNT          245 a          371 c          407 g          322 t
ORIGIN      
        1 tatgcattcg gtgcaagacg ggcaggcaga tcggcgcgag gcacccgctg gcgacatcat
       61 ggatcacatt gcggcgttct ttgacgcgcg catcgcggcg ctgacgggtg ccggtatcaa
      121 acgcaaccgc cttgtccttg atcccggcat ggggtttttt ctgggggctg ctcccgaaac
      181 ctcgctctcg gtgctggcgc ggttcgatga attgcggctg cgcttcgatt tgccggtgct
      241 tctgtctgtt tcgcgcaaat cctttctgcg cgcgctcaca ggccgtggtc cgggggatgt
      301 cggggccgcg acactcgctg cagagcttgc cgccgccgca ggtggagctg acttcatccg
      361 cacacacgag ccgcgcccct tgcgcgacgg gctggcggta ttggcggcgc tgaaagaaac
      421 cgcaagaatt cgttaactgc acattcggga tatttctcta tattcgcgct tcatcagaaa
      481 actgaaggaa cctccattga atcgaactaa tatttttttt ggtgaatcgc attctgactg
      541 gttgcctgtc agaggcggag aatctggtga ttttgttttt cgacgtggtg acgggcatgc
      601 cttcgcgaaa atcgcacctg cttcccgccg cggtgagctc gctggagagc gtgaccgcct
      661 catttggctc aaaggtcgag gtgtggcttg ccccgaggtc atcaactggc aggaggaaca
      721 ggagggtgca tgcttggtga taacggcaat tccgggagta ccggcggctg atctgtctgg
      781 agcggatttg ctcaaagcgt ggccgtcaat ggggcagcaa cttggcgctg ttcacagcct
      841 atcggttgat caatgtccgt ttgagcgcag gctgtcgcga atgttcggac gcgccgttga
      901 tgtggtgtcc cgcaatgccg tcaatcccga cttcttaccg gacgaggaca agagtacgcc
      961 gcagctcgat cttttggctc gtgtcgaacg agagctaccg gtgcggctcg accaagagcg
     1021 caccgatatg gttgtttgcc atggtgatcc ctgcatgccg aacttcatgg tggaccctaa
     1081 aactcttcaa tgcacgggtc tgatcgacct tgggcggctc ggaacagcag atcgctatgc
     1141 cgatttggca ctcatgattg ctaacgccga agagaactgg gcagcgccag atgaagcaga
     1201 gcgcgccttc gctgtcctat tcaatgtatt ggggatcgaa gcccccgacc gcgaacgcct
     1261 tgccttctat ctgcgattgg accctctgac ttggggttga tgttcatgcc gcctgttttt
     1321 cctgctcatt ggcacgtttc gcaac
//
LOCUS       DAEEUE010000317         1339 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.guided.267, whole genome shotgun sequence.
ACCESSION   DAEEUE010000317 DAEEUE010000000
VERSION     DAEEUE010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.guided.267"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            36..851
                     /gene="sul2"
                     /locus_tag="KLK84_004369"
     CDS             36..851
                     /gene="sul2"
                     /locus_tag="KLK84_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HBE2011420.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            912..>1339
                     /locus_tag="KLK84_004370"
     CDS             912..>1339
                     /locus_tag="KLK84_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HBE2011421.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAV"
BASE COUNT          248 a          375 c          399 g          317 t
ORIGIN      
        1 actcatgctc gattattatt attatagaag cccccatgaa taaatcgctc atcattttcg
       61 gcatcgtcaa cataacctcg gacagtttct ccgatggagg ccggtatctg gcgccagacg
      121 cagccattgc gcaggcgcgt aagctgatgg ccgagggggc agatgtgatc gacctcggtc
      181 cggcatccag caatcccgac gccgcgcctg tttcgtccga cacagaaatc gcgcgtatcg
      241 cgccggtgct ggacgcgctc aaggcagatg gcattcccgt ctcgctcgac agttatcaac
      301 ccgcgacgca agcctatgcc ttgtcgcgtg gtgtggccta tctcaatgat attcgcggtt
      361 ttccagacgc tgcgttctat ccgcaattgg cgaaatcatc tgccaaactc gtcgttatgc
      421 attcggtgca agacgggcag gcagatcggc gcgaggcacc cgctggcgac atcatggatc
      481 acattgcggc gttctttgac gcgcgcatcg cggcgctgac gggtgccggt atcaaacgca
      541 accgccttgt ccttgatccc ggcatggggt tttttctggg ggctgctccc gaaacctcgc
      601 tctcggtgct ggcgcggttc gatgaattgc ggctgcgctt cgatttgccg gtgcttctgt
      661 ctgtttcgcg caaatccttt ctgcgcgcgc tcacaggccg tggtccgggg gatgtcgggg
      721 ccgcgacact cgctgcagag cttgccgccg ccgcaggtgg agctgacttc atccgcacac
      781 acgagccgcg ccccttgcgc gacgggctgg cggtattggc ggcgctgaaa gaaaccgcaa
      841 gaattcgtta actgcacatt cgggatattt ctctatattc gcgcttcatc agaaaactga
      901 aggaacctcc attgaatcga actaatattt tttttggtga atcgcattct gactggttgc
      961 ctgtcagagg cggagaatct ggtgattttg tttttcgacg tggtgacggg catgccttcg
     1021 cgaaaatcgc acctgcttcc cgccgcggtg agctcgctgg agagcgtgac cgcctcattt
     1081 ggctcaaagg tcgaggtgtg gcttgccccg aggtcatcaa ctggcaggag gaacaggagg
     1141 gtgcatgctt ggtgataacg gcaattccgg gagtaccggc ggctgatctg tctggagcgg
     1201 atttgctcaa agcgtggccg tcaatggggc agcaacttgg cgctgttcac agcctatcgg
     1261 ttgatcaatg tccgtttgag cgcaggctgt cgcgaatgtt cggacgcgcc gttgatgtgg
     1321 tgtcccgcaa tgccgtcaa
//
LOCUS       DAEEUE010000318         1338 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.guided.268, whole genome shotgun sequence.
ACCESSION   DAEEUE010000318 DAEEUE010000000
VERSION     DAEEUE010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.guided.268"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            114..974
                     /locus_tag="KLK84_004371"
     CDS             114..974
                     /locus_tag="KLK84_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HBE2011422.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            1184..>1338
                     /locus_tag="KLK84_004372"
     CDS             1184..>1338
                     /locus_tag="KLK84_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HBE2011423.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPG"
BASE COUNT          365 a          311 c          324 g          338 t
ORIGIN      
        1 gacaaaaggc tgcctcatcg ctaactttgc aacagtgcct acattcaaat atgtatccgc
       61 tcatgagaca ataaccctgg taaatgcttc aataatattg aaaaaggaag agtatgagta
      121 ttcaacattt tcgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
      181 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
      241 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
      301 gttttccaat gatgagcact tttaaagttc tgctatgtgg tgcggtatta tcccgtgttg
      361 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
      421 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
      481 ctgccataac catgagtgat aacactgctg ccaacttact tctgacaacg atcggaggac
      541 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
      601 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag
      661 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
      721 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
      781 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
      841 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
      901 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
      961 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     1021 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     1081 tcccttaacg tgagttttcg ttccactgag cgtcagaccc ctaaaagaac tttccgctaa
     1141 gcgatagact gtatgtaaac acagtattgc aaggacgcgg aacatgcctc atgtggcggc
     1201 caggacggcc agccgggatc gggatactgg tcgttaccag agccaccgac ccgagcaaac
     1261 ccttctctat cagatcgttg acgagtatta cccggcattc gctgcgctta tggcagagca
     1321 gggaaaggaa ttgccggg
//
LOCUS       DAEEUE010000319         1016 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.guided.280, whole genome shotgun sequence.
ACCESSION   DAEEUE010000319 DAEEUE010000000
VERSION     DAEEUE010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.guided.280"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            58..531
                     /locus_tag="KLK84_004373"
     CDS             58..531
                     /locus_tag="KLK84_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HBE2011424.1"
                     /translation="MKVSLMAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            752..>1016
                     /gene="mobC"
                     /locus_tag="KLK84_004374"
     CDS             752..>1016
                     /gene="mobC"
                     /locus_tag="KLK84_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBE2011425.1"
                     /translation="MPVLTDEEVNTVRESCRQLGAIGRNLNQVARALNIEFRESDKLK
                     QEAIEKLAERIDQHLDHVSELFDKTWSRWHDCHFQKTTAPVDWA"
BASE COUNT          287 a          224 c          264 g          241 t
ORIGIN      
        1 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aacccaggat gagaaccttg
       61 aaagtatcat tgatggctgc gaaagcgaaa aacggcgtga ttggttgcgg tccagacata
      121 ccctggtccg cgaaagggga gcagctactt tttaaagcat tgacctacaa tcagtggctt
      181 ctggtgggtc gcaagacgtt tgaatctatg ggcgcactcc ccaataggaa atacgcggtc
      241 gttacccgct caggttggac atcaaatgat gacaatgtag ttgtatttca gtcaatcgaa
      301 gaggccatgg acaggctagc tgaattcacc ggtcacgtta tagtgtctgg tggcggagaa
      361 atttaccgag aaacattacc catggcctct acgctccact tatcgacgat cgacatcgag
      421 ccagaggggg atgttttctt cccgagtatt ccaaatacct tcgaagttgt ttttgagcaa
      481 cactttactt caaacattaa ctattgctat caaatttgga aaaagggtta acaaagctat
      541 gcaatcgacg gcaaaaagct tcgttcgctt cgcgcactac gccttttccg cgattgatag
      601 cgacgttatg tgaatattga aatgagcatt ccaaagaaag gaagtagaaa aattatcgtg
      661 ggcgaaaatg agtttttgtg gctcattagg tcaaaactca catattcgca ggactgttta
      721 ggtacagaaa tgactgcggt cgttgaaccg gatgccggtc ctcactgatg aagaagtgaa
      781 caccgtacgg gaatcgtgcc ggcagcttgg agctatcggc cggaacctca accaggtggc
      841 cagggccttg aacatcgagt tcagggaaag tgacaagctc aagcaagagg ccatcgaaaa
      901 actggccgaa cggatcgacc agcatttgga ccatgtgtct gagctgttcg ataagacctg
      961 gagccggtgg cacgattgtc attttcagaa gacgactgca ccagttgatt gggcgt
//
LOCUS       DAEEUE010000320         1001 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.guided.282, whole genome shotgun sequence.
ACCESSION   DAEEUE010000320 DAEEUE010000000
VERSION     DAEEUE010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.guided.282"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            132..923
                     /gene="aadA1"
                     /locus_tag="KLK84_004375"
     CDS             132..923
                     /gene="aadA1"
                     /locus_tag="KLK84_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HBE2011426.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          257 a          254 c          263 g          227 t
ORIGIN      
        1 aaacacacta aatttataaa aaatctaatg gcaaaatcgc ccaacccttc aatcaagtcg
       61 ggacggccaa aagcaagctt ttggctcccc tcgctggcgc tcggcgcccc ttatttcaaa
      121 cgttaaacat catgagggaa gtggtgatcg ccgaagtatc gactcaacta tcagaggtag
      181 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
      241 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa
      301 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
      361 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
      421 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
      481 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
      541 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
      601 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
      661 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
      721 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
      781 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
      841 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt tcactacgtg aaaggcgaga
      901 tcaccaaggt agtcggcaaa taatgtctaa caattcgttc aagccgacgc cgcttcgcgg
      961 cgcggcttaa ctcaagcgtt agatgcacta agcacataat t
//
LOCUS       DAEEUE010000321          991 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.guided.283, whole genome shotgun sequence.
ACCESSION   DAEEUE010000321 DAEEUE010000000
VERSION     DAEEUE010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.guided.283"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            52..711
                     /locus_tag="KLK84_004376"
     CDS             52..711
                     /locus_tag="KLK84_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HBE2011427.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT          262 a          217 c          230 g          282 t
ORIGIN      
        1 acaccatcat acactaaatc agtaagttgg cagcatcacc aggaagctaa aatggagaaa
       61 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
      121 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc
      181 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
      241 gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg
      301 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca
      361 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat
      421 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt
      481 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg
      541 gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg
      601 ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga
      661 atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta atttttttaa
      721 ggcagttatt ggtgccctta aacgcctggt tgctacgcct gaataagtga taataagcgg
      781 atgaatggca gaaattcgaa agcaaattcg acccggtcgt cggttcaggg cagggtcgtt
      841 aaatagccgc ttatgtctat tgctggttta ccggtttatt gactaccgga agcagtgtga
      901 ccgtgtgctt ctcaaatgcc tgaggccagt ttgctcaggc tctccccgtg gaggtaataa
      961 ttgacgatat gatcatttat tctgcctccc a
//
LOCUS       DAEEUE010000322          867 bp    DNA     linear   BCT 27-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF184470
            SAMN17129052-rid12249843.guided.287, whole genome shotgun sequence.
ACCESSION   DAEEUE010000322 DAEEUE010000000
VERSION     DAEEUE010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129052
            Sequence Read Archive: SRR13348970
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042413.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042413.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:41:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,377
            CDSs (total)                      :: 4,282
            Genes (coding)                    :: 3,925
            CDSs (with protein)               :: 3,925
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 159 of 357
            Pseudo Genes (incomplete)         :: 87 of 357
            Pseudo Genes (internal stop)      :: 143 of 357
            Pseudo Genes (multiple problems)  :: 31 of 357
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129052-rid12249843.guided.287"
                     /strain="SF184470"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2019-10"
                     /collected_by="LSPQ"
     gene            16..846
                     /locus_tag="KLK84_004377"
     CDS             16..846
                     /locus_tag="KLK84_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HBE2011428.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
BASE COUNT          343 a          156 c          141 g          227 t
ORIGIN      
        1 aaaccaatac ttattatgaa aaacacaata catatcaact tcgctatttt tttaataatt
       61 gcaaatatta tctacagcag cgccagtgca tcaacagata tctctactgt tgcatctcca
      121 ttatttgaag gaactgaagg ttgtttttta ctttacgatg catccacaaa cgctgaaatt
      181 gctcaattca ataaagcaaa gtgtgcaacg caaatggcac cagattcaac tttcaagatc
      241 gcattatcac ttatggcatt tgatgcggaa ataatagatc agaaaaccat attcaaatgg
      301 gataaaaccc ccaaaggaat ggagatctgg aacagcaatc atacaccaaa gacgtggatg
      361 caattttctg ttgtttgggt ttcgcaagaa ataacccaaa aaattggatt aaataaaatc
      421 aagaattatc tcaaagattt tgattatgga aatcaagact tctctggaga taaagaaaga
      481 aacaacggat taacagaagc atggctcgaa agtagcttaa aaatttcacc agaagaacaa
      541 attcaattcc tgcgtaaaat tattaatcac aatctcccag ttaaaaactc agccatagaa
      601 aacaccatag agaacatgta tctacaagat ctggataata gtacaaaact gtatgggaaa
      661 actggtgcag gattcacagc aaatagaacc ttacaaaacg gatggtttga agggtttatt
      721 ataagcaaat caggacataa atatgttttt gtgtccgcac ttacaggaaa cttggggtcg
      781 aatttaacat caagcataaa agccaagaaa aatgcgatca ccattctaaa cacactaaat
      841 ttataaaaaa tctaatggca aaatcgc
//
